LOCUS       DACDKM010000001        94264 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20834_2016
            SAMN12640370-rid9891663.denovo.001, whole genome shotgun sequence.
ACCESSION   DACDKM010000001 DACDKM010000000
VERSION     DACDKM010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640370
            Sequence Read Archive: SRR10040891
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 94264)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 94264)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742835.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742835.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:04:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,012
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 29 of 75
            Pseudo Genes (incomplete)         :: 33 of 75
            Pseudo Genes (internal stop)      :: 19 of 75
            Pseudo Genes (multiple problems)  :: 5 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..94264
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640370-rid9891663.denovo.001"
                     /isolate="E20834_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(25..1521)
                     /gene="lysS"
                     /locus_tag="IV334_000001"
     CDS             complement(25..1521)
                     /gene="lysS"
                     /locus_tag="IV334_000001"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007208013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HAP8942107.1"
                     /translation="MTQEQQHQVEDLNDQMLVRRQKMEQLREDGIDPFGKRFERTHNS
                     EELHELFDPRTKEELAEMGLTASVAGRMMTRRGKGKAGFAHLQDREGQIQIYVRKDQV
                     GDEAYEVFKHADLGDFFGVTGQIMKTDTGEVSIKASEITILSKALRPLPDKYHGLTNI
                     EQRYRQRYLDLISNRESFDRFMKRSQIISEIRRYLDGNGYIEVETPVLHNEAGGAAAR
                     PFITHHNALDIDLYLRIALELHLKRLIVGGMEKVYEIGRVFRNEGIDTTHNPEFTMLE
                     AYTAYTDFNDVMNLTEGIIRNAAEKVLGTAKITYDGQAVDLESDFKRIHMVDAIKEQT
                     GVDFWQEMTLEEALALAEKHNVEVTEAMGVGHVINEFFETFVEETLTQPTFVYGHPVE
                     VSPLAKKNPEDPRFTDRFELFIVGREFANAFTELNDPIDQRERFEAQEKERELGNDEA
                     HGVDEDFLEALEYGMPPTGGLGIGIDRLVMLLTDAQSIRDVLLFPTMR"
     gene            complement(1632..2636)
                     /gene="dusB"
                     /locus_tag="IV334_000002"
     CDS             complement(1632..2636)
                     /gene="dusB"
                     /locus_tag="IV334_000002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HAP8942108.1"
                     /translation="MEHTWKIGNVEIPNRVVVAPMAGITNSAFRVTVKEFGAGLVVCE
                     MISDQGIHFRNKKTLEMLHIEETEHPLSLQIFGGNKDSLVEAAQFVEENTAADIIDIN
                     MGCPVNKVIKAEAGAKWLLDPEKVYEMVQAVSSAVNIPVTIKMRIGWDEEHVFAVENA
                     LAAQSAGASAIAMHGRTRVQMYEGKADWEVLKEVKKHLTIPFMGNGDVKTPEDAKWML
                     DYVGADGVMIGRAALGNPWMIHRTQHYLETGELIPEPTPAEKIATAKLHLQRLADLKG
                     EKVACREFRTHAAYYLKGIPRAAKVKVAINQTEKQQEINDLLDQFVEKAEKVLLKQTL
                     "
     gene            complement(2637..3524)
                     /gene="hslO"
                     /locus_tag="IV334_000003"
     CDS             complement(2637..3524)
                     /gene="hslO"
                     /locus_tag="IV334_000003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HAP8942109.1"
                     /translation="MKDYLVKALAHDGFVRAYAVQATNTVAEAQRRHDTWHTSSAALG
                     RTLVGSLLLGATLKGEDKLTVRIQGDGPATGIVVDANGKGDVKGYIKNPHLSLPLNSE
                     GKIDVRGAVGTQGMFTVTKDLGLKEPFSGQTPIVSGEIAEDFTYYLAISEQIPSAVGL
                     SVLVNTDDSIKTAGGFMIQIMPGADEETISKIEEQLKTTPRISTLLDEGQTPEEILKG
                     LLGTEELNILEKMPVQFKCDCSKEKFAEAIIALGAEEIQAMIDEDHGAEAVCSFCNNK
                     YKYSEEELDGLKKEALGGI"
     gene            complement(3741..5852)
                     /gene="hflB"
                     /locus_tag="IV334_000004"
     CDS             complement(3741..5852)
                     /gene="hflB"
                     /locus_tag="IV334_000004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HAP8942110.1"
                     /translation="MNKKNNGMMKNALYYVLVILAMVMVVYFIFGNNGQRSSDIEYSK
                     FTEQLQAGKIKEFEVQPANGVYKITGEYKEQQTVANSGGLAILGSTDTTTTHFTTIIL
                     PNDTTLSQVTELAKENKVATTVKEESSSGIWVSLLLSFLPIVIIIFFFYMMMGQQGGG
                     GGGGGRVMNFGKSKAKEADKKANRVRFSDVAGAEEEKQELVEVVEFLKDPRRFVELGA
                     RIPAGVLLEGPPGTGKTLLAKAVAGEAGVPFYSISGSDFVEMFVGVGASRVRDLFETA
                     KKNAPAIIFIDEIDAVGRQRGAGMGGGHDEREQTLNQLLVEMDGFDGNEGVIVIAATN
                     RSDVLDPALLRPGRFDRQILVGRPDVKGREAILRVHARNKPMADDVDLKVVAQQTPGF
                     AGADLENVLNEAALVAARRNKKKIDASDIDEAEDRVIAGPAKKDRVISKKEREMVAYH
                     EAGHTIVGLVLSRARVVHKVTIIPRGRAGGYMIALPKEDQFLMTKEDMFEQIVGLLGG
                     RTAEEIIFNVQSTGASNDFEQATALARSMVTEYGMSDRLGPVQYEGNHQVFVGRDYGQ
                     TKAYSEQVAFEIDQEVRKILMEAHDKAREIIEAHRAQHKLIAEKLLEFETLDAKAIKS
                     LFEHGVMPEGTESNDYPSEKEAKTFEEAKRALEEKDAEKQAEEKRDFEEAKHELNEEN
                     KAEHDSDSQQSEDNHSDDQYK"
     gene            complement(5942..6487)
                     /gene="hpt"
                     /locus_tag="IV334_000005"
     CDS             complement(5942..6487)
                     /gene="hpt"
                     /locus_tag="IV334_000005"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HAP8942111.1"
                     /translation="MLEKDIERILISQEEIQVRCKELGKELTEIYQNTNPLVVGVLKG
                     AVPFMADIVRSIDTYLELDFMDVSSYGNATVSSGEVKIVKDLDTNVEGRDLLIVEDII
                     DSGRTLAYLVDLFKYRKAKSVKIVTLLDKPEGRVVNIEADYVGFNVPNEFVVGYGLDY
                     AEAYRNLPYIGVLKPSVYQSN"
     gene            complement(6487..7932)
                     /gene="tilS"
                     /locus_tag="IV334_000006"
     CDS             complement(6487..7932)
                     /gene="tilS"
                     /locus_tag="IV334_000006"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HAP8942112.1"
                     /translation="MNVEKGGKKAPLLQQSFIRHGNEQGFWQQGSRILAAVSGGVDSM
                     VLLHLLLQVQRQIGFSLGIVHINHQLREESAEEAAYLRSYCEEKNLPLYVSIWEDPAK
                     SAIETEARNFRYQEFTRVMEEGQYDTLMTAHHGDDQIETVLMKIVRGGQLNTFSGIKE
                     VQPFATGRLIRPLLSFSKEDLYTYAAESQLVYFEDQTNQLLDIQRNRLRHLVVPQLKQ
                     ENTQALRHFQQFSQQIQWADQVIQKYMGQLIEKEVEQWEEQFRFSAEMIEEMTDAERY
                     YFFYSFFDKAFSKTGIILKEQQMESILDQWQQKKSQWQLSIGNNWILKREYHFVVLSK
                     KEKQATSQTEDQRLLLIPEQGIYLNETEWIGLVKPENAGDIKATDSWSLFSHDIWIPA
                     DVQLYVEKRKAGDRIKLTENLTKKVSRYFIDQKVPNSQRQHAWIITDAKRNIYSLIPF
                     AFSYLSIREETDKIHYILLYKYQKEAIGRRT"
     gene            complement(8052..8522)
                     /locus_tag="IV334_000007"
     CDS             complement(8052..8522)
                     /locus_tag="IV334_000007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein S1"
                     /protein_id="HAP8942113.1"
                     /translation="MSIEVGAKLQGKVSGITNFGAFIDLGAGKTGLVHISEVSNGYVK
                     DIHDVLSVGDEVTVKVISVNDDGKIGLSIRKAQEQPAESGEESRPKKYQQKDPRDNRN
                     RPAQKKSFSRSQPSAQKEDFDSLMSSFLKDSDDRLSSLKRNTEGKRGGRGGRRS"
     gene            complement(8568..8993)
                     /locus_tag="IV334_000008"
     CDS             complement(8568..8993)
                     /locus_tag="IV334_000008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HAP8942114.1"
                     /translation="MTKKSNKIAALDTEYAKKKYAEFQQQQRQLIFRRRRLAAIFVGA
                     LVIFAIIGVQIFHDMQRMHQLNELRVEANTEMKEVNADVDQLHQDVSLLKDDEYVAKL
                     ARSKFFYSKDGEKVYPILESNTDSSTNETDQSTSSTTAK"
     gene            complement(9060..9329)
                     /locus_tag="IV334_000009"
     CDS             complement(9060..9329)
                     /locus_tag="IV334_000009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HAP8942115.1"
                     /translation="MRLDKFLKISRIIKRRSVAKEVADKGRIQINGKLAKSSSDVKVG
                     DILKVQFGNKTLEVKITELHESTKKEDAQKMYEIISENRTETLEK"
     gene            complement(9340..10935)
                     /locus_tag="IV334_000010"
     CDS             complement(9340..10935)
                     /locus_tag="IV334_000010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HAP8942116.1"
                     /translation="MDHKEMRRVMQGAFVLTIASFIAKVLSALYRIPLQNLVGDEGFY
                     VYQQVYPIYGIAMTLALSGLPQFISKYVAERKNPFERKQALQNLYPLVFWTGVFLWLF
                     VFAFSSWIAWMMGDIQLKPLIQVVSFTFLLMPGLSFYRGNFQGRFLMEPTAVSQVVEQ
                     FVRVFVIVCSAFAFRKLGWTVYQTGTAAMSGAVFGGLCAYGILHYYEQKIHGGALSFR
                     RFPLFKQPSKPLIRRFLVEGGLVSIYSGLLIFFQLIDSFFVKNALEVYGLSEYGAKIA
                     KGVYDRGQPLVQLGLVIATALSATFLPALTRHLTNRIYRQFLQTAKIYLRLTTALALA
                     ASLGLALLLPYINYALFKDYAGNAALVLFVFSIAFTAVIQAYQSIAQSKNSFRPSLKG
                     AGWGLLVKGLTTSFLTGLLGTAGASLSTLLGLGVTLWYFVRIETKELNAFWKERHFGR
                     KLMFSLLCMTVVLLLYYGVLNIVFGPVTHRSTAFFVSVIGVGIGASVFIYAVIRLRLF
                     TVREWLMLPFGKKILRLNKKIKK"
     gene            complement(10950..14471)
                     /gene="mfd"
                     /locus_tag="IV334_000011"
     CDS             complement(10950..14471)
                     /gene="mfd"
                     /locus_tag="IV334_000011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HAP8942117.1"
                     /translation="MDIIQLVGKDPQVKEWVSNLEEKMPRQLITGLAGSAKTLLFARA
                     FKKTNKNMIILMPNLYYANQLAEDLQHVIPQDQIHLFPVDEVLSAEMAFSSPEARGER
                     VATLNALQEKKAGIYLLPVASLHKRLPNKKTWLQAQLNWHIGDEIDVENLPRQLTLMG
                     YVREEMVAKPGEFSIRGSIIDIYPLTTTYPVRVELFDVEIDSMRYFEAETQRSIEKTE
                     EIWVSPTSEQVYGPEDLEHGIEQMQKMLEKRLAATTEEADREFLQDYFGQLTSSWQAG
                     VPTEEAKFYSDLLYQESVTILDYFPSNSLLVVDDYQRIMETNREIEREAAEWQTQKIS
                     ELRVFSEQTFSADIQKIVQKEKFTTTFFSLFQKGMGNLRFQAIHNFQYRTMQQFFGQM
                     PLLKTEMDRWRKQKQTVVIFIPTKDRIRKAEQMLRDEDILVVETEKNALIRGQVQLVE
                     GALQAGFELPQEKIVAITEKEIFQKTTKKQTRRQTVTNAERLKSYNELKAGDYVVHAN
                     HGIGKYIGMETLEVDGVHQDYMTILYQNDDKLFIPVTQLNLIQKYVASEAKAPRINKL
                     GGSEWTKTKRKVSSKIEDIADDLIKLYAARESEKGYAFGPDDAYQKEFENAFPYSETD
                     DQLRSAAEIKRDMEKEKPMDRLLVGDVGYGKTEVALRAAFKAIKESKQVAFLVPTTIL
                     AQQHYETMLERFEGFPVNIGLLSRFRTRKQQKETIEQLRTGQVDIVVGTHRILSKDIE
                     FSDLGLLIIDEEQRFGVKHKERLKQLRAQVDVLTLTATPIPRTLHMSMLGVRDLSVIE
                     TPPANRYPIQTYVMEKNPGAIREAIHREMGRGGQVFYLYNRVETIEQKVEEIQELVPE
                     ARIGYAHGQMTEAQLENTLFEFIEGQYDVLVTTTIIETGVDIPNANTLFVENADYMGL
                     STLYQLRGRVGRSSRVAYAYFMYEQQKILNEVSEKRLQAIKDFTELGSGFKIAMRDLS
                     IRGAGNLLGAQQHGFIDAVGFDMYSQMLSEAVSRKQGKNSQVEKTTVEIDLGVDAYLP
                     ETYVADQRQKIEIYKRIRELDSQEMLDELEDDLLDRFGEYPEEVAHLLTIGQIKMDGD
                     RALLETIRKQEAHIVFTLSKVGTKRYSVEQLFEALTATKLKASLGVEKEQMVIRLTIP
                     NQMEEAVWLQEIQKFVKALREQKYLSA"
     gene            complement(14488..15048)
                     /locus_tag="IV334_000012"
     CDS             complement(14488..15048)
                     /locus_tag="IV334_000012"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HAP8942118.1"
                     /translation="MKMIVGLGNPGTKYQYTKHNIGFMVVDKIAREHQATFKKNPFEA
                     EVAEFFHNGEKILLVKPQTFMNESGRAVGPLMTYFGIYPEELVVIYDDLDLAVGKIRL
                     RQKGSAGGHNGIKSIISHLNTNVFDRIKVGIGRPEGKKTVVQHVLSPFSKENQPLIEE
                     SMCQSVKAVEYLIEGHSFVDAMNRFN"
     gene            15401..16375
                     /locus_tag="IV334_000013"
     CDS             15401..16375
                     /locus_tag="IV334_000013"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HAP8942119.1"
                     /translation="MTAAAEKKDHQKVILVGDGAVGSSYAFALVTQNIAQEVGIVDIN
                     TAKTEGDAIDLSHALAFTSPKKIYSATYADAHDADLVVITAGAPQKPGETRLDLVNKN
                     LKINRDVVTQIVESGFNGIFLVAANPVDILTYSTWKFSGFPKERVIGSGTSLDSARFR
                     QALAELVDVDARNVHAYILGEHGDSEFPVWSHANVAGLQIYEWVKNNPEIDEEAMVNL
                     FFGVRDAAYTIIEKKGATFYGIAVALARITRAILDDENAVLPLSVFMNGEYGLNDIYI
                     GAPAVINRQGIQKVIEIPLSDSEQDRMAASAKQLKEILDEAFAKLDAE"
     gene            16545..17945
                     /locus_tag="IV334_000014"
     CDS             16545..17945
                     /locus_tag="IV334_000014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HAP8942120.1"
                     /translation="MTRSSHRKKSKHVALLSVLGVLLVLISFYTYRSTYYTKHFLPNT
                     EINGINVSNLTVEKANEKLKEAYSDKKLAIKEDGKVWQEVPKSELGYKDDFTSELSPI
                     LNEQNGWTWGMTYVSAAEKQEIDPVSQNHQKLDTTVQTLTTKLTDLNKDRTPTTDATI
                     EKAGDSFVIKPEVNGNTIDVDAAVKQLKSAVNSGKDTIELTEFKEKPKVTSEDSSLKE
                     QLASMNAIANVKATYSINGETFQIPSSDIMSWLTYNDGKVDLDTEQVRQYVTDLGTKY
                     NTSTNDTKFKSTKRGEVTVPVGTYSWTIQTDSETEALKKAILAGQDFTRSPIVQGGTT
                     ADHPLIEDTYIEVDLENQHMWYYKDGKVALETDIVSGKPTTPTPAGVFYVWNKEEDAT
                     LKGTNDDGTPYESPVNYWMPIDWTGVGIHDSDWQPEYGGDLWKTRGSHGCINTPPSVM
                     KELFGMVEKGTPVLVF"
     gene            complement(18023..18745)
                     /locus_tag="IV334_000015"
     CDS             complement(18023..18745)
                     /locus_tag="IV334_000015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HAP8942121.1"
                     /translation="MSGHSKWHNIQGRKNAQDAKRGKVFQKLSREIYMAAKAGGPDPS
                     MNPALRLVVDKAKAANMPNDNVERAIKKATSAVDETNYDEITYEGYGPGGVGILVHAL
                     TDNRNRTATNVRVAFTRNGGSLGETGSVSYLFDRKGYIAIERDGLTVDEETMFEDVLE
                     AGAEDLQTSPEVFEIYTAPEDFAAVRDELEKEFTLAQAELTMIPQTTIDLNEEQKEQL
                     ERLVDKLEDDDDVSEVFTAAEM"
     gene            complement(18824..19330)
                     /locus_tag="IV334_000016"
     CDS             complement(18824..19330)
                     /locus_tag="IV334_000016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HAP8942122.1"
                     /translation="MNSSKKQMKNGNIYLVIALIVVIILFYSSSQTYGQQSQVSRLES
                     WLPNQPFKDTLAGIRFNYGGSMVSINHLGYAKFLEFFLRKGAHFGTYFILGGSLFLGV
                     FPKLKIWWLTAILAWLSATGYAGLDEFHQMMTGGRTPMFQDVMLDSIGALTAVILCVL
                     FAAFKKRL"
     gene            19453..21252
                     /locus_tag="IV334_000017"
     CDS             19453..21252
                     /locus_tag="IV334_000017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family oligoendopeptidase"
                     /protein_id="HAP8942123.1"
                     /translation="MSYSLNWNLDSIFSGGSHSDALNQRMKQLEDQMNEYYHRVTKWS
                     PSSDNAEQLNAILQLQETITNGFTQCSSYITALLSANVNDSDAKVLSGKLYAMLPRLQ
                     SAETVLSKKFAEISDNDWNQMLSQSSFETIAFRLNEIRRDGSQLLSEAEENIINTLSL
                     DGLNAWSSHYDTIVASISIPFEQDGQVVELSAGQAFNKMMGDPDPKVRETLFAAWEEA
                     WKEKAPLFTDTLNHLDGFRLSDYELHGVTDFLQKPLEYNRLKKETLSVMWDTIQKNKQ
                     PLVNYLTRKANLFGKEKMEWQDQDAPIILGDLKEKTFSFDEAAAFIIENFNKFSPKMA
                     KFAQSAFEKSWIEAEDRPGKRPGGYCTELPETKESRIFMTYSNSVNEVATLAHELGHA
                     FHSSTMWDLPSLNREYAMNVAETASTFAELIVADATLKVAKTKEEKINLLDTKLQNAL
                     AMFMNIHSRFIFENRFYEARQEGLVSEDKITEMMVEAQKEGYQGALATYHPYFWAAKL
                     HFFIDDVPFYNFPYTFGYLFSLGIYAYANQKGANFEEEYIHLLRDTASMTTEDLAKKH
                     LNVDLTQPDFWQAGIDMVTEDIQTFMELTEEFI"
     gene            complement(21320..22894)
                     /locus_tag="IV334_000018"
     CDS             complement(21320..22894)
                     /locus_tag="IV334_000018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8942124.1"
                     /translation="MKKSLISAVMVCSMTLTAVASPIAAAADDFDSQIQQQDQKIADL
                     KNQQADAQSQIDALESQVSEINTQAQDLLAKQDTLRQESAQLVKDIADLQERIEKRED
                     TIQKQAREAQVSNTSSNYIDAVLNADSLADAIGRVQAMTTMVKANNDLMEQQKQDKKA
                     VEDKKAENDAKLKELAENQAALESQKGDLLSKQADLNVLKTSLAAEQATAEDKKADLN
                     RQKAEAEAEQARIREQQRLAEQARQQAAQEKAEKEAREQAEAEAQATQASSTAQSSAT
                     EESSATQSSMTEESSSATQSSATEESTTPESSTEESTAPESSATEESTTAPESSATEE
                     STTVPESSATEESTTVPESSTTEESTTPAPTTPSTDQSVDTGNGTGSSTPAPTPTPTP
                     EQPKPVTPAPAPSGSVNGAAIVAEAYKYIGTPYVWGGKDPSGFDCSGFTRYVYMQVTG
                     RDIGGWTVPQESAGTKISVSQAKAGDLLFWGSQGGTYHVAIALGGGQYIHAPQPGESV
                     KVGSVQWFAPDFAVSM"
     gene            complement(23149..23658)
                     /gene="mreD"
                     /locus_tag="IV334_000019"
     CDS             complement(23149..23658)
                     /gene="mreD"
                     /locus_tag="IV334_000019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HAP8942125.1"
                     /translation="MLKKETMKYYLPIVLFFLMLIDGHLTRMLGEWSKGTYMSNAHFL
                     ILALLCCSMAFEKRYLLITTIVLGAIYDAYYIGVIGIYAVALPLIVWLMYVMKDVIHV
                     NIFTEFFSMIIFVTGYELFTMVVQLIFKLAVVNNTYFITRFLGPTLLLNMIIFVLFIF
                     PFKKLFSDE"
     gene            complement(23662..24516)
                     /gene="mreC"
                     /locus_tag="IV334_000020"
     CDS             complement(23662..24516)
                     /gene="mreC"
                     /locus_tag="IV334_000020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HAP8942126.1"
                     /translation="MKKFNPNKNIIITLVIVIIVVTILSVTAAQRANEGKPNFFQSIV
                     NDSVAMVDKTISAPVRWVRNGVDSVHNLFSTYAENERLKEKIDSYDDVSQQNKNYERE
                     IEALKKELELNQTLTSYEKVTANVITRSPDSWQDMLIVDKGSKDGIEANMAVMSQKGL
                     IGRVIEVNTASSKIELLSSSNESSNHFPVRVSSANGEAFGLLKNYDEKLHALVVTQLT
                     GDTDIKEGDVVQTSGLGGNSPANLPIGTVIKTKPDSYGLDREVYVKPYAEMYDVSVVT
                     IVQRLAED"
     gene            complement(24671..25309)
                     /locus_tag="IV334_000021"
     CDS             complement(24671..25309)
                     /locus_tag="IV334_000021"
                     /EC_number="2.7.6.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine diphosphokinase"
                     /protein_id="HAP8942127.1"
                     /translation="MKILLAAGGNPQNWPQSLLSKYDAYVGIDRGALYLLQAGYPVDL
                     AVGDFDSLSNEEQKEVFAKAKEYTRSPAEKDDTDTQLGILKTFERYPQAEVTLIGATG
                     GRLDHLLANLWLGMEPRFKPFAHQLIIRDCQNYLSYYLPGEHIVRQIPDMKYLAYCCL
                     TPVSNLTLKGSKYVLENKEVEYPTSYASNEFLSDTAEFSFSDGMIAVIQSKD"
     gene            complement(25310..25960)
                     /locus_tag="IV334_000022"
     CDS             complement(25310..25960)
                     /locus_tag="IV334_000022"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HAP8942128.1"
                     /translation="MKIAPSILSADFANLQRDIELVEKGGADYIHVDVMDGRFVPNIT
                     LGPNIVQAIRPVTKLPLDVHLMIVDPENYIPAFAKAGADIITVHVEATPHIHRALQMT
                     KSLGVKAGVVINPGTPVSMIKHVLPLADQVLVMTVNPGFGGQSFIEETVEKITELADL
                     RRENNWTYAIEVDGGIVPETAKICKDAGADVFVAGSYIYGAENPVGQIERLKEALD"
     gene            complement(25973..26872)
                     /gene="rsgA"
                     /locus_tag="IV334_000023"
     CDS             complement(25973..26872)
                     /gene="rsgA"
                     /locus_tag="IV334_000023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HAP8942129.1"
                     /translation="MALIKGQIRKAISGFYYVYANGETYQTRGRGNFRNRKITPLVGD
                     QVIFESENQTDGYLLEILPRKNQLVRPPVTNVDLGIIVTSIVEPAFSYNLLDRFLVTL
                     EYESIEPIIFLTKTDLSDDPEKIEEIKQTYTKIGYPVIVPNHTGDTTELIRYFPERLS
                     VFMGQSGAGKSTLLNQISPELNLATGEISDALGRGRHTTRHVELIPIYGGLVADTPGF
                     SSIDFLTIEAGELPRQFPEFVEASVHCKFRECMHAKEPGCEVKHRVETGGIAQTRYDN
                     YLQFLQEIEKRKPMYAKKEGKRK"
     gene            complement(27037..29106)
                     /gene="pknB"
                     /locus_tag="IV334_000024"
     CDS             complement(27037..29106)
                     /gene="pknB"
                     /locus_tag="IV334_000024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Stk1 family PASTA domain-containing Ser/Thr
                     kinase"
                     /protein_id="HAP8942130.1"
                     /translation="MIELGKKLNGRYQITGNIGSGGMANVFLAHDLILDRDVAVKVLR
                     FDFQNDQTAIRRFQREALAATELVHPNIVSVYDVGEEDNMQYLVMEYVKGMDLKRYIQ
                     THYPVPYETAVNIMQQILSAISLAHNHQIIHRDLKPQNVLIDNEGVVKITDFGIAIAL
                     SETSITQTNTMLGSVHYLSPEQARGSMATKQSDIYALGIILYEMLTGSVPFDGESAVT
                     IALKHFQDDLPSIKALDPNVPQALENVILRATAKEPADRYKSAEEMSDDLSTVLSPAR
                     ANEEKWQPHVMDNETKVITPLTEDAPMPDSFKSMPLPKDKPENETLPEEEEVLPDPKK
                     KGQKKWWLILLAILAVLGIGTAVFFASGGRGEVVVPDVSDLSEASARETLAKAKLEVA
                     TKTEEIADDKIEEGNVVKTDPAAGTTVKQKREVTLYISSGTKKIKLDDYSGKSYEDAE
                     ASLKELGFSADLIQKEEEFSSEVESGMIISQSPSEDTEVDPKTDTITFTVSKGPETVT
                     LSSHVGENYETVRNWLVTQGFNINNISATYDYSDTVPSGSIISQDPASGEVVAEETYI
                     NFVVSQGTEPKLNDISGYTKSEAQSYLASVGAEYIGHETSEYSNTVDKDKVIRTVPGA
                     GTTITKGMVVNVIYSKGPDPASSSSEKDSSDSSSSSSSSSSTSNSSKEESSSSTSSSA
                     STSESSK"
     gene            complement(29103..29843)
                     /locus_tag="IV334_000025"
     CDS             complement(29103..29843)
                     /locus_tag="IV334_000025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Stp1/IreP family PP2C-type Ser/Thr phosphatase"
                     /protein_id="HAP8942131.1"
                     /translation="MQIEYQSDVGRRRNTNQDYASVFTSRSGLKLAVLADGMGGHRAG
                     DIASQMAVTNLGEAWEKQEAVDDEKIAQWFIQSIQEENMLIYQRGQEQPEYNGMGTTI
                     VAAALSDDWFTIANVGDSRAYLIREDKIMQLTEDHSLVNELVKSGEISEEMAVNHPRK
                     NILTRSVGMPGTVEVDVSTHLWQLKDRLLLCSDGLTNMLSEEEIGTIINQEGPLSHKV
                     TELIDRANEAGGADNITVLLIEYKEDSV"
     gene            complement(29856..31214)
                     /gene="rsmB"
                     /locus_tag="IV334_000026"
     CDS             complement(29856..31214)
                     /gene="rsmB"
                     /locus_tag="IV334_000026"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HAP8942132.1"
                     /translation="MKKIPKKMKKSVRYMALLTLERVNKGGAYSNLLLNEMIKQSELN
                     QKDVGLFTELIYGTISHQRLLEFYVAPLIAKAKKVDDWVKMLLYLSVYQLEFLDKVPA
                     HAILNEAVEIAKVKGNPGTGKFVNGVLRNYQRQGAPDLQGIADPIERLSVKISLPLWM
                     TKRLVDQIGYEETEKLGLSLYQPSHASARVDTRRLSRDQAITVLQEEEITAEKSQVSP
                     YGIVAKKGHLASSSLFHDGVMTIQDESSMLVAPSMQIEPEHHVLDACAAPGGKTTHIA
                     SFLDPSCGGEVVALDVHEHKVKLIEDNAARLGVSSAVYAQKMDAREVSKQFEAEQFDR
                     ILVDAPCSGLGLLRRKPDIRYKKQPDELANLPKIQLEILESCAPTLKSSGILTYSTCT
                     ILKEENQDVVSAFLERHPDFERIDVLTNPMLQSSIEEKMLTLYPHQFYTDGFFICCLR
                     KK"
     gene            complement(31214..32152)
                     /locus_tag="IV334_000027"
     CDS             complement(31214..32152)
                     /locus_tag="IV334_000027"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HAP8942133.1"
                     /translation="MTKLVFMGTPAFSVPILEGLIESGYEIQAVVTQPDRPVGRKRVI
                     TPTPVKKAALKHGIRVLQPEKISGSPEMEEIIELVPDVIVTAAFGQFLPEKLLQVPKL
                     GAINVHASLLPKYRGGAPVHYAIMNGEKETGVTIMEMIKKMDAGGIYAQESIPITKQD
                     DVGTMFEKLSLLGRKLLLETLPNILDGQKPVPQDESEATFSPNITREQEAIDWHKTAE
                     EIDNQVRGMRPWPIAFTTYEGIRWKLLSVEVLAETTDKAPGTIIRKDKKNLWIACGKQ
                     TVLAINELQPAGKGKQAVHDFLNGSGQQVQIGQQVK"
     gene            complement(32145..32636)
                     /gene="def"
                     /locus_tag="IV334_000028"
     CDS             complement(32145..32636)
                     /gene="def"
                     /locus_tag="IV334_000028"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HAP8942134.1"
                     /translation="MRYPILIHPNDKLKRTAQPIDVITDETIALLDNLYETMIANDGI
                     GIAAPQVGQNKRIAVIEVDEGEKFELINPEIIEAKGESLDVEGCLSIPHVYGTVKRAD
                     EVTVRYYDRDGEEIEVTAFGYLARAFQHEIDHLDGILFIEKMIQQIPEEELEEYMEEH
                     LDD"
     gene            complement(32651..35059)
                     /gene="priA"
                     /locus_tag="IV334_000029"
     CDS             complement(32651..35059)
                     /gene="priA"
                     /locus_tag="IV334_000029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HAP8942135.1"
                     /translation="MTLTAEVIVDVPTMQTDQPFTYLVPSEMETALQVGMRVEVPFGN
                     GNRHVQGFVMAIQKSEESANPSLKAVIRLLDLAPVVNEELLSLADYMKKITYAFKITC
                     LQTMLPSVMKAEYDKLIYPLADTPEVQVLFDQREVISWKEAEEEGSLSQLIRWRQEQL
                     VDIKYEVHTRNKVKTIRLVRSLLTEKQIEEEWAKLRQNAKKQKELLLCLSELSQEEPI
                     AYFKNKGISTAVLNQGKEKGWLEFIESERYRDPYKDRVFDQTTALELNAEQKNAVEQI
                     ITAGQQQKDQVFLLEGITGSGKTEVYLQAIADVLSENKTAIMLVPEIALTPQMVERFK
                     GRFGESVAVLHSGLSQGEKYDEWRKIEREEAQVVVGARSAIFAPLKNIGLIIIDEEHE
                     SSYKQDETPRYHARDLAIWRSKYHHCPIVLGSATPSLESRARAQKGVYQLLQLNHRAK
                     AAAQLPAIEIVDMREEFQNHRTSTFSANLQEKIQNRLDKKEQTVLLLNRRGYSSFVMC
                     RDCGFVLPCPNCDISLTLHMDTRSMRCHYCGHEEPIPNRCPNCGGNKIRYYGTGTQKV
                     EEELRELYPQARILRMDVDTTRRKGAHEQILQKFGAKEADILLGTQMIAKGLDFPEVT
                     LVGVLNADTSLNLPDFRSSEHTFQLLTQVSGRAGRAEKAGEVVIQTFNPQHYAIELAK
                     KQNYEQFYQQEMHVRHRGGYPPYYFTVKITASHPEEQVAAKKIFQIANQLKEVLSPQS
                     LLLGPTPSMILRVKNRYYYQLIIKYKHEPNLPHVLDEILNGSQKEQRQGLFVAIDNEP
                     LNFI"
     gene            complement(35312..35617)
                     /locus_tag="IV334_000030"
     CDS             complement(35312..35617)
                     /locus_tag="IV334_000030"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HAP8942136.1"
                     /translation="MMLKPSIDSLLDRVNSKYSLVILASKRAHELDASAQPTLDSFDS
                     VKSVGQALEEIDAGNVVNDPHPELKRERLKMEEEERQAQKEREQHELESRIREEQKM"
     gene            complement(35622..36236)
                     /gene="gmk"
                     /locus_tag="IV334_000031"
     CDS             complement(35622..36236)
                     /gene="gmk"
                     /locus_tag="IV334_000031"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010766465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HAP8942137.1"
                     /translation="MSERGLLIVLSGPSGVGKGTVRKAIFESEDNDFQYSISMTTRKM
                     REGEVEGVDYFFRSKEEFESMIEAGEMLEYAEYVGNYYGTPLSYVNKTLDEGKDVFLE
                     IEVQGAKQVKEKVPDGVFIFLTPPDLAELKSRIVGRGTDADEVIEKRMKVAKEEIEMM
                     ALYDYAVVNDEVPLAVERIKNIIASEHFRVDRVIGKYIKMLKEM"
     gene            complement(36723..37007)
                     /locus_tag="IV334_000032"
     CDS             complement(36723..37007)
                     /locus_tag="IV334_000032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8942138.1"
                     /translation="MKYYDVTFHELSGKTVVKRDIPSEKNGFDVWKDACADYNENELF
                     ILINDGAYVTMNRKFIVRIDTEEVEDPTEKARSRKDEIMGVVNTLSNMGF"
     gene            complement(37025..37915)
                     /locus_tag="IV334_000033"
     CDS             complement(37025..37915)
                     /locus_tag="IV334_000033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YicC family protein"
                     /protein_id="HAP8942139.1"
                     /translation="MKSMTGFGKAIRETKAYQLDIEIKSVNQRFLDVQIRSPKILNFV
                     ENDFRQLIKQQLSRGRIEVFVNLAYIGKNEKQIVINWELIDSLITQLTDGIQQRYGSQ
                     NQVSISRMAERFVLDENFVTVEEKPEESMEELAQLALDTLKSALDSIEASREKEGQAL
                     ALVLKKNTEEFKEVLKKLDSFVEIYEKEFQIRYQKKLEEFLGAKVDEQRLLTELAILL
                     ERGDIHEELDRLAIHVQKLDELIEAQCPVGRELDFLIQEMNREINTIGSKSNAIEIKN
                     QVVQSKTILEKIREQVQNIE"
     gene            complement(37935..38153)
                     /locus_tag="IV334_000034"
     CDS             complement(37935..38153)
                     /locus_tag="IV334_000034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8942140.1"
                     /translation="MAQEFSYEIVEEIAILSENNKGWRKELNLVSWNGRPPKFDLRDW
                     APDHEKMGKGLTLTNEEFEQLQKAIENM"
     gene            complement(38123..39823)
                     /locus_tag="IV334_000035"
     CDS             complement(38123..39823)
                     /locus_tag="IV334_000035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HAP8942141.1"
                     /translation="MEIKKVYFSQKMSLYSPIQQAKQPLPFFQSHQESRDAFWRSAKK
                     QCAFKAVSKKYTVYFTIAKSRGDVIFNNLLIEGETFEWKKFFRYMEACARFFIKENCC
                     FLFQSPLSEEWLRIFHKNGYQGTTQLNKKLVYHTALVLGGGGAHGAYQIGVWQALKEH
                     DINFEIITGTSVGALNGALILQGDMKKAVNLWKKLSTRQVLALPEMAAVEDLRERFYR
                     ERRQMTKTALIEGGVSSAPLEKMVKDNLDLSLLKHNPKIRLYTVSTKLPELAEVVTDI
                     QQTKTEEIPDWILASASFYPAMAYRKIGKNKYADGGYRNKIPIDIAINKGATEAFVVD
                     VQGPGPAKKIRMPDIFIHWKCQTLWTLGSFLLFDSQRNQLNLQLGYLEMKKRLGYYYG
                     NWYTFHSVKPARTYWRGFLSYLTKEIQLELSFFKSIKFWQKLRNLYKNRVVPETCGLA
                     MLELLAKKHFLLPNEIYQVDMMIQMICQQDIKSMPDDLLAQIGQLSTEEWRRYRKYQQ
                     KIQNERTKTVYFDYLLQGKRKDRLQHELLKQPVDTLLILYLYYLKEEQPWHKNFHMKS
                     "
     gene            40090..40863
                     /locus_tag="IV334_000036"
     CDS             40090..40863
                     /locus_tag="IV334_000036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8942142.1"
                     /translation="MTESVYVHIDTTSNAVLTKGLTATDFANSIVHFPQNLLLLDPSA
                     SAGEYESHTGLKVIRGTENIQRFFSSSRNRRGFDELKWIDFTDLTMLKELTPLEISEL
                     LYFGHMKTHLHSPFFYKLQNNFVYFDLNDQLNRIYYRYLDEFYRIFASKLSSILSDKI
                     NVKKSFFKKETPVEKISVELLKEMRNLMQEGIIFSFDQVGLIDGKYRIPIYVIEDYIW
                     KLKGTRYREEEIIATLIYSVTDRHWQLVEDADLSYLPLK"
     gene            complement(40944..41513)
                     /gene="thiT"
                     /locus_tag="IV334_000037"
     CDS             complement(40944..41513)
                     /gene="thiT"
                     /locus_tag="IV334_000037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupled thiamine transporter ThiT"
                     /protein_id="HAP8942143.1"
                     /translation="MGKNRVWVEGTIVAALAMVLSLIPIQIGSSFSISLGQIPLTLFA
                     LRRGTKAGMLAGFIWGILHFPLGQVYYLSVVQVLIEYPIAYTFAGAAGLMASNVQKGY
                     VDDNTKQIRKSIVFGALIGALSRYFWHFIAGVVFWGAYALWGMNPWLFSFVMNGASGL
                     STAIVTIIVLLGIQQTTPSIFVPDKRQEL"
     regulatory      complement(41579..41681)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(41687..42658)
                     /locus_tag="IV334_000038"
     CDS             complement(41687..42658)
                     /locus_tag="IV334_000038"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016618611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HAP8942144.1"
                     /translation="MSKHYFDPRLKIFALNSNRPLAEKIAAAVGVELGKSSVTQFSDG
                     EIQVNIEESIRGSHVYVIQSTSSPVNDNLMELLIMIDALKRASAKTINVVMPYYGYAR
                     QDRKARAREPITAKLVANMIQKAGATRMLTLDLHAVQIQGFFDIPVDHLMGAPLIANY
                     FLEKGIKGDDVVVVSPDHGGVTRARKLAEFLKSPIAIIDKRRPKANVAEVMNIIGQVK
                     GKTCVLIDDMIDTAGTITLAANALKEAGATSVYASCTHPVLSGPALQRIEDSAIERLV
                     VTDSIYLPEDRKIDKIDEVSVGGLMGDAIKRIHENKPVSPLFETKNK"
     gene            complement(42752..44125)
                     /gene="glmU"
                     /locus_tag="IV334_000039"
     CDS             complement(42752..44125)
                     /gene="glmU"
                     /locus_tag="IV334_000039"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HAP8942145.1"
                     /translation="MDARYAIILAAGKGTRMKSKLYKVLHPVSGKPMVEHIINRVSET
                     KPDEVITIVGHGAEQVKAQLGERSKYALQAEQLGTGHAVLQAASFLEGKKGTTLVISG
                     DTPLLTTETLNNLFEYHQGKNASATILTAQAENPTGYGRIIRDHIGIVEKIVEQKDAT
                     PEEALVQEINTGTYCFDNEALFDALSKVGTNNAQGEYYLTDIIEILKEEGHTVAAYQT
                     DDFEESMGVNDRIALAKANEIMRKRINQMHMVNGVSFVDSATTYIDAGVEIGPDTLIE
                     AGVQIQGNTVIGSDCVIGSHSKIVDSRIEDHVVIENSVIESSHVKKHADVGPYAHLRP
                     KAEIGENVHIGNFVEVKNAQIGKGTKVGHLTYVGDATLGEEINVGCGVVFVNYDGKNK
                     HHTTVGDHSFIGSSTNIIGPVEVAKNSSIAAGSTITDNIPEYALAIARARQVNKEGYA
                     KKLPYLN"
     gene            complement(44628..45446)
                     /gene="purR"
                     /locus_tag="IV334_000040"
     CDS             complement(44628..45446)
                     /gene="purR"
                     /locus_tag="IV334_000040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pur operon repressor"
                     /protein_id="HAP8942146.1"
                     /translation="MKVRRSERLVDMTQYLLNHPHELISLTSFAERYESAKSSISEDL
                     GIIKKTFKERGYGTLETIPGAAGGVLFIPEISYEEAKKYIESLAERLSEQDRLLPGGY
                     VYLSDLLGEPELLRQVGRIIASKYLGEKIDAVMTVATKGVPIAQAVSYYLNAPFVIVR
                     RDSKITEGSTVSVNYVSGSSERIEKMELSKRSLKRGSRVLVVDDFMKGGGTVNGMKSL
                     IEEFESELVGITVFAESKFNGHRAIDDYTSLLYVKDVDTQTKNITVVPGNYFSE"
     gene            complement(45613..46437)
                     /locus_tag="IV334_000041"
     CDS             complement(45613..46437)
                     /locus_tag="IV334_000041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HAP8942147.1"
                     /translation="MALLSYEFMRRAFLAAIFIAGIAPMLGVFLVIRRQSLMADTLSH
                     VSLAGVALGFFLNLNPTLTTMLVVVLAAVILEYLRTIYRTYSEISIAILMAGGLALAL
                     VLMNLSGGNSATSIQSYLFGSIVTITWEQVIFLGILFVAIGILFFLFKRPMYVLTFDE
                     DTAHVDGLPVHLMSMMFNVITGVAIAVMIPIAGALLISAIMVLPAAIGMRLGKSFNVV
                     IAISVCVGFLGMLSGLTSSFYLDTPPGATITLVFIALFLIVNIIKRILVAFRRKNN"
     gene            complement(46410..47090)
                     /locus_tag="IV334_000042"
     CDS             complement(46410..47090)
                     /locus_tag="IV334_000042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HAP8942148.1"
                     /translation="MRYIEISDLTFYYDDEPVLEDVSYHVDAGEFVILTGENGAAKST
                     LVKASLGLLKPSKGMIKIAEKNQEGKKLSIGYIPQQVASFNAGFPSTVIELVRSGRYP
                     RGRWFKRLSEKDHQHVQRALEAVGMWDMRNRRIGELSGGQKQRISLARIFATDPDLFV
                     LDEPTTGMDEESRNEFYRLLRHSAHVHGKSVLMITHDHEDIKQYADRQIRLVRKEDSQ
                     WRCFHMNS"
     gene            complement(47113..48051)
                     /locus_tag="IV334_000043"
     CDS             complement(47113..48051)
                     /locus_tag="IV334_000043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein"
                     /protein_id="HAP8942149.1"
                     /translation="MKKYLLTATVMIGALLFAACGNTNKEADKKEDLTIVTTFYPMYD
                     FTKEIVGDEGNVKLLIPAGTEPHDFEPSAKERAEISDADVFVYNSSDMEFFVDSLKDS
                     VDSKQTLMIEAAKGIDRLESQEADEHEESEEGHGHSHEYDPHVWLDPVLAIKEVRTIA
                     EELGEKYPDKKETFTKNADAYIKKLEALDQKYSDELKDATNRTFVTQHAAFAYLANQY
                     DLKQVAISGVSPDQEPTPSRLAELKEFVKKNNIKVIYFEENASSKVAETLSNETGVKL
                     EVLNPLESLTNEQIKAGENYISVMEKNLKALKESIN"
     gene            complement(48296..49147)
                     /locus_tag="IV334_000044"
     CDS             complement(48296..49147)
                     /locus_tag="IV334_000044"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HAP8942150.1"
                     /translation="MELIEKAPAKINLGLDVLYRRQDGYHELEMVMSSVDLADHLFFE
                     EITEDKIIVETNKAFLPVDERNNVYQAALLLKNKYNIKTGVKIFVKKNIPVAAGLGGG
                     STDCAAALRGINQLWNLGCSLEELAEIGLEVGTDVPYCVYGSTAFVGGKGEEIQVLPS
                     MPQCWVVLVKPRMSVSTRTVFREVDTQAIQHQDIHGLAEAIREQDYQQMIKKMGNSLE
                     DITGKRHPVIHQIKERMIKYGADAALMSGSGPTVFALCQHYSRAQRVYNGLKGFCEEV
                     YLVRTLQ"
     gene            complement(49301..51874)
                     /gene="mprF"
                     /locus_tag="IV334_000045"
     CDS             complement(49301..51874)
                     /gene="mprF"
                     /locus_tag="IV334_000045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysylphosphatidylglycerol
                     flippase/synthetase MprF"
                     /protein_id="HAP8942151.1"
                     /translation="MKNKRYLMLQWIKEHSLILKLIFFGSVLVFVANQVANIANGMSW
                     QDIWQRMGQQSRMTILLMILAGLLGVTPMLLYDWVTIRVLEKQGKPKMERKEFLLAAW
                     VTNTINNLAGFGGVVGASLRASFYGKQTNRKMVLATVSKVALFMLTGLSVWSFLTFID
                     VFFIQSESIFRSYWIWLFGGSFLAPVIFFFAYLKRKTLFFEFYPKGLLGLLLASFGQW
                     TGALAVFLIIGKLMRIDISMLSIYPMFMIATLIGMLTMVPGGMGTFDVLMILGMSQLS
                     VRQDVAVVWLLYYRLFYYVLPFMTGLLLFITHTGVKINRFLDNIPRLFFQKVAHIVLV
                     SAVYFAGIMMVLLSTITNLSAISRLFQVLLPFSFNFLDQTVNLLVGFLLLGLARALSV
                     KVKKAFLPTIALLSFGILNTITRTLSWQLILVYVLILLAVWLSRKEFYRTKFVYSWGA
                     IIFDASLFGFLFIVYAIAGYHSGRFLVGRITGNHFILFPSDDVWFSGLIGLGISLLAL
                     VALYQYLAEDTTKLGSEYEKDRFDRLLETYGGTATSHQLRLSGYSYFYYQEDDQDQVV
                     FGYQIKGNKCFVLGDPVGNPKMMRAATNQFMKQADCLGYQLAFYKISESYVVMLHELG
                     FHFTKVGEAGIVDLATEDLSRLSQQLEYQRLMKEGYHFTWYDQLPEELHQAVNEVSQK
                     WLQGAREKYFSVGRFDKSYVLSSGVGIVRKDQKVVGFITAQPMTKKQAGYDLLRVLPK
                     ESEELADYLLANLFIVYEKMGYSEANLGLAPLANVGETDFSFFQEKVMHIVYNYGNFF
                     YSFQSAYEKKLKYVDHWEGRYFAYRKGSNFGFATMQLLSLIGKEKGNVPSLAEEVLTE
                     L"
     gene            52015..52821
                     /gene="proC"
                     /locus_tag="IV334_000046"
     CDS             52015..52821
                     /gene="proC"
                     /locus_tag="IV334_000046"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HAP8942152.1"
                     /translation="MKVGFIGTGNMAQAIIDGLLKKGTVSKEEVFVSSGHFENAQKFA
                     AETGVHACKSNQEVASQSDIVLLAVKPKIIEKVLEELREYSQKVLFISIASGKSLIDL
                     ETALEDPQASIVRVMPNVNVSVGAGVSAICKNEAATEEQLQQATMIFEAIGSVYPLAE
                     SDFSTFIGLAGSSPAFVYMLIDAMGRTGVLNGLSKQTATEIAAKAIMGSCQKFLSSDK
                     NPWELVDQVSSPGGTTVAGVVALEEAGFIPAIIKGINTTIEKDLEMLNKK"
     gene            complement(52900..54456)
                     /gene="rny"
                     /locus_tag="IV334_000047"
     CDS             complement(52900..54456)
                     /gene="rny"
                     /locus_tag="IV334_000047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HAP8942153.1"
                     /translation="MVFNILLAIIGLIVGLGLGFMVAKSRHEKEIAGAQSSAAGIIDS
                     AKKEAETLKKEALLEAKEENQKYRSEVESELRESKLELKSQENRLIQREQTLNRKDDS
                     LEKRENSLESKEEALSSKQQLIEEREKDVEKMIEDQQKELEKIAGLSKEDARDVIMKS
                     TEEELNHELTLMVKESEQRAKEEADRKAKNLLSLAIQRCAADQVSETTVSVVTLPNDE
                     MKGRIIGREGRNIRTLETLTGIDLIIDDTPEAVVLSGFDPIRREIARMTLEKLIQDGR
                     IHPARIEEMVEKSRKEMDERIREYGEEAAFEVGAHTLHPDLIKIIGRLRFRTSYGQNV
                     LKHSVEVAKLAGILAAELGEDIQLAKRAGLLHDIGKALDHEIEGSHVEIGAELAAKYK
                     ENPVVINAIASHHGDVEATSVISVLVAAADALSAARPGARSESLENYIRRLRSLENIS
                     NSFAGVESSYAVQAGREVRVMVKPEEISDLDAVRLVRDIRKRIEEELDYPGHIKVIVI
                     RETRATDYAK"
     gene            complement(54867..55916)
                     /gene="recA"
                     /locus_tag="IV334_000048"
     CDS             complement(54867..55916)
                     /gene="recA"
                     /locus_tag="IV334_000048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HAP8942154.1"
                     /translation="MADDRKAALDAALKKIEKSYGKGSIMKLGEKIDQQISTIPSGSL
                     ALDVALGVGGYPRGRIIEVYGPESSGKTTVALHAIAEVQKNGGTAAFIDAEHALDPQY
                     AQKLGVNIDELLLSQPDTGEQGLEIADALVSSGAVDIVVVDSVAALVPRAEIDGEMGD
                     SHVGLQARLMSQALRKLSGSINKTKTIAIFINQIREKVGVMFGNPEITPGGRALKFYA
                     TIRLEVRRAEQLKQGTDIVGNRTKIKVVKNKVAPPFKIAEVDVMYGLGISQEGELLDM
                     AVEKDIVDKSGAWYSYKEDRIGQGRENAKIYMANHPEMMAEVSALVRAAYGIGEEVAV
                     PEDEKGQEELPLVEE"
     gene            complement(56025..57266)
                     /locus_tag="IV334_000049"
     CDS             complement(56025..57266)
                     /locus_tag="IV334_000049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HAP8942155.1"
                     /translation="MKAEIIAVGTELLLGQVVNTNATFLSEQLADLGIEVYYQTVVGD
                     NQQRLEELIALAETRSELILLCGGLGPTEDDLTKEATAAHLGKSLIQNTEGYKKLLAY
                     FETTHRKMTKNNLQQSQIIEGGVPLPNRTGLALGTFYQTDTHAYILLPGPPNELKPMF
                     VEQVRPLLEERFPSEEKLISKVLRFYGIGESRLVTELKDLIETQINPTIAPYAKPNEV
                     TLRLTVKTNDVQAGNQALLALEEKIQERVGEYFYGYGDDNSLAKVVVELLKENKQTVT
                     AAESLTAGAFQAALGDIAGVSEVFPGGFVTYSLQTKAGFLEIDPELLAEYGTVSKECV
                     EQMAIQAREIARTDYSVAFTGVAGPDTLEGQSAGTVWIAISSKDGVVSEKYHFARDRA
                     AIRYNAVMQGFDLLRKQLLRK"
     gene            complement(57506..58084)
                     /gene="pgsA"
                     /locus_tag="IV334_000050"
     CDS             complement(57506..58084)
                     /gene="pgsA"
                     /locus_tag="IV334_000050"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010754872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HAP8942156.1"
                     /translation="MNLPNKLTVLRICMIPIFILVVGLPLDWGQFNVGDTTLAVTQLV
                     GAVIFALASITDWLDGKIARARGLVTNFGKFADPLADKMLVMTAFIMLVDQGKAPGWV
                     VAIIVCRELAVTGLRLLLVEHGEVMAAAWPGKVKTATQMVAIILLFINNIPFSLIGIP
                     VDQIMLYACLIFTIYSGVDYFIKNSGVFKGSM"
     gene            complement(58293..59177)
                     /locus_tag="IV334_000051"
     CDS             complement(58293..59177)
                     /locus_tag="IV334_000051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP8942157.1"
                     /translation="MAFETIGEKLRQARLNKKISLDELQQITKIQKRYLEAIDNDDFD
                     QLPGKFYVRAFIRQYAEAVGEDGDHLVDVFDGKSKLTGSVPERPQPETVSTSRKAMHQ
                     EESHPSRFWTSLPVILLGLVALAIVVVVGYMTWQDRQADPIIGDTASSLKVEGSVEES
                     SSSEQSTQTKESSTVESTTKSSEEKKMAIATGEDSGSAIAVNITDATKPVTLEFTAVN
                     RVWLGVLVDNAYVYQGTLAANETQSTVLPETATNATITIGAASNATIKANGESVPVNP
                     GENNQSPKNVNLTLQYAE"
     gene            complement(59955..61241)
                     /locus_tag="IV334_000052"
     CDS             complement(59955..61241)
                     /locus_tag="IV334_000052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HAP8942158.1"
                     /translation="MNKTEYEQINETLYHEVLPNGLTVYLLPKNDYHKTYGLFSTNYG
                     SIDNEFIPYGEKEKVKVPDGIAHFLEHKLFEKEDGDVFQLFGKQGASANAFTSFTKTS
                     YLFSTTDQVEKNLTTLIDFVQAPYFTEETVNKEKGIIGQEIQMYEDDPNWRMFFGILN
                     NLYPTHPLHIDIAGTVESIDKITAQDLYTCYRTFYQPSNMVLFVVGKMEPEKLMKLIR
                     ENQEAKNFPPKQEIVRYFPENTKEIIKQSALEAAITRDKFVLGIKGLDTLPQEGTELL
                     RYKTAINLLFQMILGNTSRNYLAMYNQGIIDDSFGFEFSLDREFHFADFSGDTDEPEK
                     AAEKVKEIILGFADDPEVSETNLDLLKKKMLGQYFQSLNSIEYIANQFTQSLFGDRTL
                     FDLPEIIDSIQMKDVLAAGEAFISEEAFSEFYMRPK"
     gene            complement(61234..62502)
                     /locus_tag="IV334_000053"
     CDS             complement(61234..62502)
                     /locus_tag="IV334_000053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HAP8942159.1"
                     /translation="MVYQLAEGVNLHVLPTKQYKTIRIFIRFTARLQQEVITKRSLLS
                     SMLETNSLNYSDQTKLSAQLAELYGASFGLSVRKKGNLHWLNAGISFVNGEYVNDPNL
                     FSQAVDFLKEVLFYPNIKNHQFDQLTFDLEKNNLRLYLESLKEDKQTFASYALQELYF
                     ENSPEQKIPGLGVVEDLDKITASSLASYYQEMMANDQIDIFVVGDVDPDKAAEAVGQL
                     PFESRETVYPDLFYTQPQVNIVKERQVREPIVQAKLNLAYQTNVYYDEPERFALMVFN
                     GLFGGFPHSKLFMNVREKESLAYYASSSVDTFRGFMSVQTGIDEKNRNQVLHLIHEQL
                     ESLRNGEITELELSQTKAMLRNQYLLSLDSPQAAIEASFLDSWLPETKLSDEEWLKRM
                     ESVTIKEIQQVAEQIELQAIFFLAGGNANE"
     gene            62754..63215
                     /gene="mscL"
                     /locus_tag="IV334_000054"
     CDS             62754..63215
                     /gene="mscL"
                     /locus_tag="IV334_000054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="large conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HAP8942160.1"
                     /translation="MIKEFKEFIMRGDVLDLAVGVVIGSAFTSIVNQVVQGLITPLIG
                     WFVAMITGTSDLEGALSILDWSPTKGVTFAFGNVISAIITFLITGFVLFLVVKAANRA
                     KNLRSKEEQVEVEETPTAEDYLSDIRDLLAKQVAEDNTHPVEQTNLSDTSK"
     gene            complement(63275..63721)
                     /locus_tag="IV334_000055"
     CDS             complement(63275..63721)
                     /locus_tag="IV334_000055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP8942161.1"
                     /translation="MEYQVKKFEELSTAEFYAIVKERIAVFVVEQQCPYQEVDDIDGE
                     AWHTFFQDTDGKILAYTRVYEEAESIHFGRVLVHQDNRKDGLGRKIVQETINMIQKNF
                     PEKPIVIGAQEYLQAFYESFGFKAISDVYLEDDIPHIDMKKEQVAS"
     gene            complement(63814..64059)
                     /locus_tag="IV334_000056"
     CDS             complement(63814..64059)
                     /locus_tag="IV334_000056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8942162.1"
                     /translation="MPTTLASIKKDLEGRIGSPIMLVAQTGRKRQTERRGVLTETYPS
                     VFVVDLDPEENSFERVSYSYSDVLTRTVEIEFLTEAV"
     gene            complement(64174..64752)
                     /locus_tag="IV334_000057"
     CDS             complement(64174..64752)
                     /locus_tag="IV334_000057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HAP8942163.1"
                     /translation="MSEELLRAARNGDEEAFAALYQKYFPLLLRLKNTYYLRNYVSED
                     WEQEARIALYQALRTYEKSKGVSFGSYYKSVISNQIYSLLRKQNALKRRDQKDEVSID
                     QKIETEGPDFLAEITLQEPLAIQHLLLKEAIENCEIQFSKIEAKIFHYYMQGVDFVDM
                     AECLAMDHKQVMSAYGRVKLKIKKHLKFYNEE"
     gene            complement(64829..65359)
                     /locus_tag="IV334_000058"
     CDS             complement(64829..65359)
                     /locus_tag="IV334_000058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HAP8942164.1"
                     /translation="MKKQLLIVDGYNMIGSWPELVQLKKQDKMADAREALLHRLSNYA
                     KYEGMEVIVVFDAQLVPGIQQNYKKYQLDVVFTKEDETADSYIERIAGQLNDRLTQVT
                     VATSDLAEQWMIFSQGALRTSAYELYKTIKKTEKTIAQENKDMHFIDFRRNSPWNEEQ
                     LKKLSQKLDDLMRKNK"
     gene            complement(65363..66223)
                     /gene="rlmB"
                     /locus_tag="IV334_000059"
     CDS             complement(65363..66223)
                     /gene="rlmB"
                     /locus_tag="IV334_000059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HAP8942165.1"
                     /translation="MGKERTNKHQSKRGFEDKKQNRYSNKKQTGRNQSVSDQNEEALE
                     DNFVFGHHAAVEALQQGRGNKLFLQEDSRGEKVEELKEIAREQAVPVKWVPKQKLETL
                     SDHGVHQGVVLAITPYEYLSLDQLIERTKEKKEDPFFLILDSIEDPHNFGSILRTADA
                     SGVDGVIIPKHRAVGITPIVTKTSTGAVEHVPVARVTNLVQAVQQLKETGFWVFGTDM
                     TGTNFQKWNAKGSIALVIGNEGKGMSPGLKKEMDELLTIPMTGHVQSLNASVAAGLLM
                     YQAFSQRQGE"
     gene            complement(66216..66611)
                     /locus_tag="IV334_000060"
     CDS             complement(66216..66611)
                     /locus_tag="IV334_000060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HAP8942166.1"
                     /translation="MRDYTQLNGLALAYVGDAIYEIYVRDHLIDQGETKPNKLHRAAT
                     NYVSANAQAFLIQQMLEEHLLTEEEELYYRRGRNAKSHTSAKNADVTTYRVATGFEAL
                     MGYLHLTKQTERMEELIRWCIQKVGEKNG"
     gene            complement(66608..68020)
                     /locus_tag="IV334_000061"
     CDS             complement(66608..68020)
                     /locus_tag="IV334_000061"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HAP8942167.1"
                     /translation="MIKIYNTLTRKKEEFIPIQKGHVGMYVCGPTVYNYIHIGNARST
                     IAFDTIRRYFEYRGYKVDFVSNFTDVDDKIIRTANELGITAPEVADRFIRAFEEDTKA
                     LNVEPATLHPRVMDHMPDILAFIQVLVEKGYAYESSGDVYYRTRKFENYGKLSDQSID
                     ELEVGASQRTGEEQQQKEDPLDFALWKQAKPGEISWDSPWGKGRPGWHIECSVMATKH
                     LGETIDIHGGGQDLEFPHHENEIAQSEAKTGKKFANYWMHNGYVTIGEDDEKMSKSLG
                     NFITVHEMIKNVDPQVLRFFMATTQYRRPIRYSETTMKEAATNLQRLKNTFDNLKFRQ
                     ETASENLADDADKIALLENLEQRFIEEMDDDFNAANGITVVYELAKWLNTYLDQPKVS
                     AAVLAQGEALFSQWLSIFGILFTAEELLDEEIEQLIEERNQARSNRDFVRSDEIRDLL
                     KEKGILLEDTPQGTRWRREA"
     gene            complement(68017..68553)
                     /gene="cysE"
                     /locus_tag="IV334_000062"
     CDS             complement(68017..68553)
                     /gene="cysE"
                     /locus_tag="IV334_000062"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HAP8942168.1"
                     /translation="MSWFTRAIDAAKRNDPAARSTAEIVLTYPGFHALFWHRFSHFLY
                     QHRLFLLAKINAQFWRFLTGVEIHPGATIGTGVFIDHGMGIVIGETAEIEDDVILFHG
                     VTLGGTGKETGKRHPTVKQGAMLSANAQILGPVTIGKNAKIGAGAVVLKDVPDDATAV
                     GVPAKVVRIKGEKVRRSE"
     gene            complement(68875..70335)
                     /locus_tag="IV334_000063"
     CDS             complement(68875..70335)
                     /locus_tag="IV334_000063"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HAP8942169.1"
                     /translation="MTKVRVRYAPSPTGHLHIGNARTALFNYLFARHNDGDFIIRIED
                     TDQKRNIEDGEKSQLENLAWLGMNWDESPENPGEYGPYRQSERKEIYQPLIDQLLASN
                     RAYKCYCTEEELEAEREAQRARGEMPHYAGTCANLTPEEQAEKEAQGLESVVRFRVPR
                     NTEYAFTDMVKGAISFESDNIGGDFVIQKRDGMPTYNFAVAVDDHLMKITHVLRGDDH
                     IANTPKQLMIYEAFGWTPPTFGHMTLIINSETGKKLSKRDESILQFIEQYRELGYLPD
                     AMFNFIALLGWSPVGEDEIFEHQELIKMFDPKRLSKSPAAFDAKKLQWVNNHYIKAMD
                     LDAFTDMCLPYLIADGRVAENPDEKTIEWVKKIVSLYQPQMSYAAEIVDLSTLFFNEH
                     PVLNEAAKEVLAGETVPTVLHAFKAQLEQMEVVDAPTVKAAIKAVQKETGVKGKNLFM
                     PIRVAVSGQMHGPELPETIELLGREKSLAHLNQVLN"
     gene            complement(70505..71662)
                     /locus_tag="IV334_000064"
     CDS             complement(70505..71662)
                     /locus_tag="IV334_000064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN/TRAM domain-containing protein"
                     /protein_id="HAP8942170.1"
                     /translation="MQKRVITLLMIIVGASLGISFLPSAWQAIGQQSNGWLNNTFTNS
                     LLGALIFFLLSLLLAKHISAIIKQVEQKLSEVSLTYLLFGAIGAIIGLTLGVVISTPL
                     YNMEIPFVNSVLPILLMIILGYLGLRMGTTRIEEWKKVFGSRSKKIEQETASQVESQL
                     LERKSGENFHKYKILDTSVIIDGRIYDITKTGFLEGTLMIPNFVLYELQYIADSGDSL
                     KRVRGRRGLDILNALQKEEGISVEMYDGDFEDISEVDSKLIKLAKLLDGVIVTNDYNL
                     NKVSEFQNVPVLNINALANAVKPVVIPGETMNVLVVKAGTERQQGVAYLDDGTMVVVE
                     DGQHYMNERIEVVVTSALQTAAGRMIFAKPAHAGRGIDNKKETEKKTKQSK"
     gene            complement(71696..73066)
                     /gene="radA"
                     /locus_tag="IV334_000065"
     CDS             complement(71696..73066)
                     /gene="radA"
                     /locus_tag="IV334_000065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HAP8942171.1"
                     /translation="MAKKSKVQFVCQNCGYVSPKFLGRCPNCGKWNTMVEEIEQDTTD
                     RRTRTSLTGEKAKPTKIADVVPKKEPRIKTKLEELNRVLGGGVVPGSMVLIGGDPGIG
                     KSTLLLQVSQQLAAIGGKVLYVSGEESAEQIKLRAERLGSINTEFYLYAETDMNEISR
                     AIEHISPDYVIIDSIQTMTQPDITSVAGSVSQVRETTAELLKTAKTNGIAIFIVGHVT
                     KEGSIAGPRMLEHMVDTVLYFEGEQHHSFRILRAVKNRFGSTNEIGIFEMHEHGLEEV
                     ANPSQIFLEERLDGATGSAIVVAMEGTRPILVEIQALVTPTMFGNAKRTTTGLDFNRV
                     SLIMAVLEKRAGLLLQNQDAYLKAAGGVKLNEPAIDLAIAVSIASSYKEKGTQPTECF
                     IGEIGLTGEIRRVSHIEQRVKEVQKLGFTKVYLPKNNLGNWEAPKGIEIVGVATLAET
                     LKRVFR"
     gene            complement(73129..73656)
                     /locus_tag="IV334_000066"
     CDS             complement(73129..73656)
                     /locus_tag="IV334_000066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HAP8942172.1"
                     /translation="MRKRGFEVISKYKDQNINLPQRATHHAAGYDFEAAEEVVIPSIW
                     KAGWKKGLETVADGLKKDVSENEKQLKPTLVPTGIKAYMGEDEFLQLANRSSNPLKRF
                     LVLTNGVGVIDSDYYNNEANEGHIMFQFTNFGFKDVVIKKGERIGQGIFLPFLKADQD
                     QTTAQRQGGFGSSSK"
     gene            73886..74692
                     /gene="proB"
                     /locus_tag="IV334_000067"
     CDS             73886..74692
                     /gene="proB"
                     /locus_tag="IV334_000067"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HAP8942173.1"
                     /translation="MRKEIKNAKRIIVKVGTSTLIYPNGNINLAAIDQLAFVLSDLRN
                     RGKEVILVSSGAVGVGMHKLHLSERPTLIPEQQAAAAVGQAELMNIYNQRFSVYNQQT
                     AQILVTRDVVDYPESRKNVRNTLEQLLIMDTIPIVNENDTVAVDELDHLTKFGDNDQL
                     SAIMAKLVAADLLIVLSDIDGFYSDNPTTNPEAQMYRYIDKIDEKILAQAGGAGSTFG
                     TGGMQSKLKAASRIFEANSAMILANGKNPAIIFDILAGKEVGTLFKEDLK"
     gene            74689..75936
                     /locus_tag="IV334_000068"
     CDS             74689..75936
                     /locus_tag="IV334_000068"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HAP8942174.1"
                     /translation="MTDLMQLGQQAKDSAQQLALMPTNRKNELLVQMAQTIKKNQQAI
                     IEANHKDLEKAVENNISETMQDRLQLTPERINAMATEIEKIASLDDPIGKVDEMWTNT
                     DGLRIGKKRVPLGVIGIIYESRPNVTTDAASLAFKSGNAVILRGGKEAFFSNQLLVQL
                     LQQVLVSAGESPYAIQFVDDTSHETAGQLMRLTEYLDVLIPRGGAKLIQRVKEQATVP
                     IIETGTGNNHVYIDKEAQLSMAVNIIVNAKASRPSVCNAAETLLIHSEIAPFFLPAIE
                     KELVEHGVSLRADARALEYLETAALAEDTDWDTEYLDYILAVKVVDSLDEAIAHINRH
                     NTKHSETIVTDSYAASQRFLNEVDAAAVYVNASTRFTDGSVFGFGAEIGISTQKLHAR
                     GPMGLNELTSTKYIIYGDGQIRE"
     gene            76012..76356
                     /locus_tag="IV334_000069"
     CDS             76012..76356
                     /locus_tag="IV334_000069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8942175.1"
                     /translation="MFEILMIFFIYLISLNIAAFLGVGILSLFFQFKKRSIGSQREKW
                     SQYFDKIGQKGLVTRLHISYMVALCLLATINYYSFFDHSIAYTITLLIAGIFHLSYKY
                     QLNKNHLNRTFR"
     gene            complement(76473..76799)
                     /locus_tag="IV334_000070"
     CDS             complement(76473..76799)
                     /locus_tag="IV334_000070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8942176.1"
                     /translation="MLEEYFQEVGLTLVELPIEFSDDRLIYRGYIKELPIINAEGETR
                     KAMQQKIAGMYQVYREQLLAESAEEEKEKTMNLSMDELLRYYDGETFDGFSLLNEEET
                     DLPGSW"
     gene            complement(77414..78616)
                     /locus_tag="IV334_000071"
     CDS             complement(77414..78616)
                     /locus_tag="IV334_000071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP8942177.1"
                     /translation="MEILENQIDIQEQTNEETKGKSSALVVAATCMIAFMGVGLVDPI
                     LKTIAAQLNATPAETTLLFTSYMLVTGVVMLFTGFLSSRIGLKKTLMTGLFIIVVFAG
                     LGGLSGNIGMLIGLRAGWGVGNALFVSTALATIVSILVGNTEKAIMMYEAALGCGMAV
                     GPLVGGVLGSGSWRYPFFGVAFLMFCGFIALAVLLPETEKPKRKVGLWEGVKALSNHK
                     LRSVGLIALLYNFGFFTLLAYAPFLLIGYSELEVGFVFFGWGVLLAIASIFVAPALER
                     KFTTYRTILAAFVLFIICLLLLGAGTSMPVLVPISIVLAGFFQGIINTLLTTIAMEIP
                     GLQRNVASSSYSFVRFFGGALAPFIAGVIGEHIGESYAFYFAGAIVLISVCCIIYHRN
                     YFATNEEE"
     gene            78733..79248
                     /locus_tag="IV334_000072"
     CDS             78733..79248
                     /locus_tag="IV334_000072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HAP8942178.1"
                     /translation="MNTLAYILLCVLLSKPRSGYELKQLITIFWEAHHSQIYTTLAKL
                     DQQGYVTVLDKDEHSQKKIYHLTDEGRALVEEWIKEETPAPTQKDEFLAKIYAFSTLD
                     KTTATGLLFTREQQLNKIMQKNQKKLNDLSLDKKQEFGRYVVIQRRIMLCQQELQWCQ
                     QVREQMQQFFE"
     gene            complement(79447..79893)
                     /locus_tag="IV334_000073"
     CDS             complement(79447..79893)
                     /locus_tag="IV334_000073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2798 domain-containing protein"
                     /protein_id="HAP8942179.1"
                     /translation="MPTNKKEGIIFTTMMCFLMVLGMSAYNLWLHQDLHLSSLLIGLV
                     PGFVVAFILDVFIIGVLAKKIAFKLPVNPESKVQMILTISCLMVLGMVTCMSLFGVIM
                     EQGIPDQLFSAYMNAWKMNVIAALPLQLIIVGPLSRSVLKAIQSNE"
     gene            complement(79940..81418)
                     /gene="msr"
                     /locus_tag="IV334_000074"
     CDS             complement(79940..81418)
                     /gene="msr"
                     /locus_tag="IV334_000074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Msr family ABC-F type ribosomal protection
                     protein"
                     /protein_id="HAP8942180.1"
                     /translation="MENLAVNITNLQVSFGNQLELSIDSLRVYQQDRIGIIGENGVGK
                     STLLKLIAGELFPDHGKIQTEITFNYLPQLTYLAEAKDLNLELASHFQLRLEETSERK
                     WSGGEERKIELIRLLSSYEQGMLLDEPTTHLDRKSIDRLIEELRYYYGTLVFVSHDRY
                     FLDELASKIWEVKDGEIREFSGNYSAYLTQKELEKKTQLREAESIMKEKKRLEKSIQE
                     KKKQAEKLEKVSSKKKKQQIRLDRLSSSKQKDSVQKAIQKNAKTLERRLQKIGETTKP
                     QQMKQIRFPVPKSLELHSRYPIMGQNVQLERSGRTLLVNGDFQFSLGKKIAIVGENGS
                     GKTTLLEHIRKQGEGILLSPKVSFQVYQQKGYQMTSEESIIRFVMRQTEFSESLVRSL
                     LNHLGFAQETLTKPLCTLSGGEATRLTIALLFTKPSNVLLLDEPTNFIDMATIEALEK
                     LMQIYPGTILFTSHDSYFVERTADEVYEIKGQKIKKVLTRNF"
     gene            complement(81772..82401)
                     /locus_tag="IV334_000075"
     CDS             complement(81772..82401)
                     /locus_tag="IV334_000075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HAP8942181.1"
                     /translation="MEQKNDFSTIMTGRRSVRVYDETYKIPHEEMLEMIKEATTAPSS
                     VNMKPWRFVVAESEEAKEILRPLIRFNTRQNDTSSAMVMIFGDMQCYEYGEEIYNQAY
                     ESGKMPKEVRDQQLAAIIPYYKSFSREEMNDVVKVDSSLAAMQFMLVARDHGYETNPI
                     GGFEADQLAEAFGLDKDRYVPVIILSIGKAVEEGYESVRLAPEKITMFE"
     gene            complement(82385..82849)
                     /locus_tag="IV334_000076"
     CDS             complement(82385..82849)
                     /locus_tag="IV334_000076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAP8942182.1"
                     /translation="MSLEEISHLLYQIKLADQKIVQIFEENIGISLTRYEIMMRLKEE
                     NPLLQSSLQEKLQIDQGAVTRHLKVLEANGYVSRQRNPQNNREVYVALTEKAQKKIKH
                     CEMDHKDTGSVLSPAFSDVEMKQFLTLLDKLNTQLCNITTETKEVNNNGTKK"
     gene            complement(83336..84604)
                     /locus_tag="IV334_000077"
     CDS             complement(83336..84604)
                     /locus_tag="IV334_000077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-glycoprotein
                     endo-beta-N-acetylglucosaminidase"
                     /protein_id="HAP8942183.1"
                     /translation="MKAKNLLFSVILLGNLAVANTTVLAEESTETVATQTSSSSETGI
                     ENDTESTLQSATVSENTISTEEPTTETTEQTQTEDSTSAEAQNNPENAEEITNQLTDG
                     QTLTHTHQADLSEFENYTGYAFYARSSANSQQAFIDSIASTAQSLASANDLYASVMIA
                     QAIVESGWGNSTLASAPNYNLFGIKGSYNGQSVTMPTSEYVNGQWITVNAAFRKYPSY
                     KESLQDNVTVLKTTSFQPGVYYYSGAWKSNTNSYKDATAWLTGRYATAPNYGSTLNNV
                     IETYNLTQYDTAPTSTSPMYRLYNRHTGEHLYTLNAGEKNYLPTVGWEYEGIAWQAPN
                     SGQPVYRLYNPYSGDHHYTMAQSEINSLTKIGWRYEGLSFYSGGSKPIYRLFNPNEKT
                     GTHHYTLSAKERDWLTPMGWRYEGIGFYGY"
     gene            complement(84835..85572)
                     /locus_tag="IV334_000078"
     CDS             complement(84835..85572)
                     /locus_tag="IV334_000078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAP8942184.1"
                     /translation="MNDLSEKVIVIMGASSGIGEATTKLLAEKGAKLVIAARREDRLK
                     AIKESLPEAELYIQTADVRDFAQVQAVIDLAMEKFGRIDVLYNNAGIMPTAPLVEGHR
                     DEWQNMLDINIMGVLNGISAVLPIMEKQKSGHIISTDSVAGHVVYPDSAVYCGTKFAV
                     RAIMEGLRQEQRQNNIKSTIISPGAVQTELYQTISDKKAALELHEAQKEWGLTSEDIA
                     SAVAFAIETPDRMSVSNMIIRPTTQEV"
     gene            complement(85639..86208)
                     /locus_tag="IV334_000079"
     CDS             complement(85639..86208)
                     /locus_tag="IV334_000079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar O-acetyltransferase"
                     /protein_id="HAP8942185.1"
                     /translation="MTSLIEKQIVNKEIRVTDPLFEVIHQIQAENEEPLAALNTGYHE
                     PADIRKRLENIISDKVDDTVTVLLPFYTDFGKHISIGKNVFINRQVMFVDLGGICLED
                     SVLIGPRVNLITVNHLTDPKERRGLSVKPIHIKKNAWIGAGATILPGVTIGENAIVAA
                     NATVTKDVPDNTIVAGIPAKIVKPVERII"
     gene            86336..87208
                     /locus_tag="IV334_000080"
     CDS             86336..87208
                     /locus_tag="IV334_000080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAP8942186.1"
                     /translation="MDIRILTYFLAVAREKNISRAAKSLHITQPTLSKQLKDLEEELG
                     VTLFTRGSREIQLTDSGRYLYSKGKEILSLVEKTANNLTNDAIISGEIYIGGGETKAI
                     SFISDSLHSLIKAHPDIQFHLYSGNADDIIDKLDSGLLDFGLVIEPTNKQKYQSFRLP
                     AQDRWGLLIHRNHPLAKQTVIHPQELKTVPLIISRQTSVDSQLAEWLGFSIDQLSIVG
                     TYNLLYNASLMVDNGTFGALALDGIINTYGTEKVFIPLDPPLQASLNLIWKKDQPLSP
                     AARKFLDVLTEKYS"
     gene            87550..88422
                     /locus_tag="IV334_000081"
     CDS             87550..88422
                     /locus_tag="IV334_000081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAP8942187.1"
                     /translation="MKIKKIFNQNAVLVDDGGQEKVAIGKGIGFDRKRNDLIFDRDVE
                     RIFVMEPEGQIKLQNLLNQIDERFLFAAETIIDHAETVLMEKLNEHVLIALTDHIAFS
                     AENINNGIVIRNKLLREIEVLYSDEFSIAQWAVEYLTKELGIPYTYDEAGYIAIHIHS
                     GRSGRNNNHRSIREVTIVSDIIHLVESELETDIHSEPFSLNYSRLVNHLRLLLQRTHA
                     QQYAVLDTEIVEMVKRKYPESYKISKKIRVLLTKEYQLAITKEELGYLAIHIERLRSA
                     IVQTNVNNHEEEKN"
     gene            88419..90377
                     /locus_tag="IV334_000082"
     CDS             88419..90377
                     /locus_tag="IV334_000082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP8942188.1"
                     /translation="MKAYLQRMGRSLQLPVAVLPAAALLVGIGNWWASYSNDIVAHFL
                     QAGGNAVLGQLPLLFAVGLALGMSVDKSGAAALAGLVAFEVPVNVLKTDSVATLLNIK
                     PESVDPSFAQISNVFIGIIAGLIAAALYNRFHEAKLPMALSFFSGKRLVPILAAFVML
                     LISAVLLLVWPPVYSALVSFAKFILSLGWGGAGLYGFFNRLLIPTGLHQAMNSVFWFD
                     VAGINDIGKFLASEGTKGVTGMYQAGFFPIMMFGLPAGAYAIYRNARPENKAKTASLM
                     LAAAFASFFTGVTEPLEFSFMFVAWPLYVLHAIFTGISLAVSAFFHWTAGFAFSAGFV
                     DYFLSLKNPIANQPLMLIVQGLVTAVIYYFGFNFAIKKFHLMTPGREEADLSDDDTAT
                     TNSDNKYAAQASKIYAALGGADNVTSIDNCTTRLRLQVKDTGLIDQNKIKATGVPGMK
                     IIDGKNAQVIVGTEVQFVADEMAKLHGGAATRPAQTNTVVKTETFAGGTIEQDIYAIA
                     NGKLIPITEVSDDVFAEKMMGDGYAVLPENGEIFSPIAGTITNIFPTKHAVGIQTDAG
                     IEVLLHMGINTVDLKGEPFTLYVKEGQKVARGQLIALVDLAAIQSAGKNTDMVVVFTN
                     GDKVQSLEIEPARDVKANDKIGSVSNKA"
     gene            complement(90709..91560)
                     /locus_tag="IV334_000083"
     CDS             complement(90709..91560)
                     /locus_tag="IV334_000083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAP8942189.1"
                     /translation="MHYLVTGATGGFGGYALEFLKKMVPQNEIYVLVRSEEKGEALKE
                     AGFNIRIGDYDDLDSMKEALKGIDRLLFVSGVPGNRQAEHKNVVKAAQAAGVSYIAYT
                     SFADADHSISVLAPDHQFTEKMIKDSGISHTFLRNNWYVENELPIIGQALKTGQFVYA
                     AGNGKTGWALKREYAEAAAKVLVESDSPEILELSGNLTDYEELAKALERATGKKLEII
                     EASDAAFVENLKEAGFPQEAADMFLAIQHDIKKDQLAISSDDLEKVLGKPLTSLEAAL
                     KEILDQE"
     gene            91683..92150
                     /locus_tag="IV334_000084"
     CDS             91683..92150
                     /locus_tag="IV334_000084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HAP8942190.1"
                     /translation="MKYSLQFSDAIHVLAYIEMFQDTRSLSSEMIASSIETNAANVRK
                     IMSQLKKSGLIFTKTGKPQPSLAKSPDQISLLDVYRSIEGNTQLIQVDPKTNPNCVVG
                     ANIQEALEESYARLQKKAEEEMAKITLDSLIHKIAVLEKEKRPENTGLVERFL"
     gene            complement(92413..92895)
                     /locus_tag="IV334_000085"
     CDS             complement(92413..92895)
                     /locus_tag="IV334_000085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HAP8942191.1"
                     /translation="MAKYRIEEKPDFTVTGIGVELKSSNTDFVGLAAEKQAFEQKVIG
                     NGTIDALKQAAENHYFFTVNEPYNGKMMYYVGVQTSQDVPNTTRIIPFPKGTYLVVIA
                     LAESPEELRNDLTNTAFGHVLREVEDYAYVGGPNAVVEMGVTNGKYMGEIWIPVVNKP
                     "
     gene            complement(92995..93858)
                     /locus_tag="IV334_000086"
     CDS             complement(92995..93858)
                     /locus_tag="IV334_000086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HAP8942192.1"
                     /translation="MLKDLNQLIDYIEEHLTEGLSYKIIAENTGISEYQLKRIFTFIA
                     GMSLMDYIKNRRLALANRDLLDGSRVTETAFRYGYETVEGFSRAFRDWSGYLPSEISR
                     YGIQKSFPRLTFFIDVRGGKSMEFRIEKKEQFYVAGVAKKVPVKFEGVNQEIQKLAQT
                     ITETQREEMHKLGDLYPNQVINVSYGFDGARLEEKGSLMHMIGFATTKENPYADLEML
                     AIEEHTWAIFPNKGPFPQTLQDTWGKIYAEWLPSSDYELVEAPEISFTKWGEDFSNVY
                     SEIWIAVKAKK"
BASE COUNT        26337 a        20305 c        16934 g        30688 t
ORIGIN      
        1 attccttaga cagcttttat tcgattatct cattgttggg aatagcaaaa catcccgaat
       61 tgattgtgca tcagttagca acattactaa tctgtcgatc ccgatgccta gaccaccagt
      121 tggcggcata ccgtattcta aggcttccaa gaagtcttcg tctacaccgt gagcttcgtc
      181 atttcctagt tcacgttctt tttcttgcgc ttcgaatctt tctctttgat caattggatc
      241 gttcagttct gtaaaggcat tagcaaattc gcgccccact atgaatagtt cgaatcgatc
      301 tgtgaatcga ggatcttctg gatttttctt cgctaaaggc gaaacttcga ctggatgtcc
      361 gtatacaaac gtcggttgtg tcaatgtttc ttcaacaaat gtttcaaaga attcattgat
      421 aacatgtccg acacccatag cttcggttac ttctacattg tgtttttcgg ctaaagctaa
      481 tgcttcttcc aatgtcattt cttgccagaa gtctacacca gtttgttctt tgattgcatc
      541 taccatatgg atgcgtttaa agtcactttc taagtcgaca gcttgtccat catatgtgat
      601 ttttgctgtt cctagtactt tttcagctgc gttacggata ataccttctg tcagattcat
      661 tacatcattg aaatcagtat atgctgtgta tgcttctaac atcgtaaatt ctggattatg
      721 tgtagtatca attccttcat tacggaaaac gcggccgatt tcataaactt tttccattcc
      781 gccaacaatt agtcgtttta aatgaagttc tagcgcaata cgcaaatata aatcaatgtc
      841 cagtgcatta tgatgcgtga taaatgggcg tgcggcagct ccacctgcct cattgtgcaa
      901 tactggtgtt tctacttcaa tatagccatt gccatctaaa tagcgacgga tttcgctgat
      961 aatttggctt cgtttcataa atcggtcaaa gctttcgcga ttactgatta aatccaagta
     1021 gcgttgtctg tagcgttgtt caatattcgt taagccatgg tatttatctg gcagtggacg
     1081 aagtgctttt gacaaaatcg tgatttctga agctttgata gaaacttcgc ctgtgtctgt
     1141 tttcatgatt tgtccagtta caccaaagaa atctccaagg tctgcatgtt tgaatacttc
     1201 ataggcctca tcacctactt ggtcttttct tacataaatt tgaatttggc cttcacgatc
     1261 ttgtagatgc gcaaatccag ctttcccttt tcctcggcga gtcatcatac gaccagctac
     1321 actagcggtc aatcccattt ctgctagttc ttcttttgtt cttggatcaa atagttcatg
     1381 caattcttct gagttgtgcg tgcgttcaaa tcgttttcca aatggatcga ttccatcttc
     1441 acgcaattgt tccatttttt gacgacgtac aagcatttga tcatttaaat cttctacttg
     1501 atgttgttgt tcctgtgtca caaaagttcc tccattctca tgttaatctc tttcctataa
     1561 tgccaatgaa acacaaaaaa agcaagccga acttggaatt tcggcttgaa aattcacgaa
     1621 aaacttagaa atcacaacgt ttgtttcaat agtacttttt ccgctttttc tacaaattga
     1681 tccagcaggt cattgatttc ttgctgtttt tctgtctgat tgattgctac tttcactttc
     1741 gctgctcgag gaatcccttt taaataataa gccgcatgtg ttctgaattc tctacaagct
     1801 actttttctc ctttgagatc tgctaagcgt tgtaaatgaa gtttagctgt agcaattttt
     1861 tcggctggtg ttggttcagg aatcagttcc cctgtttcga gataatgctg tgtgcggtga
     1921 atcatccatg gattgcccag cgctgcacga ccaatcatca caccgtctgc accgacatag
     1981 tcaagcatcc attttgcatc ttctggcgtt ttgacatccc cgttccccat aaatggaatc
     2041 gtcaaatgtt ttttcacttc tttcaatact tcccagtccg cttttccttc atacatctga
     2101 acccgtgtac gtccatgcat agcgatggca cttgctccag ctgattgtgc tgctaatgca
     2161 ttttctacag caaagacgtg ctcttcatcc caaccaatac gcatcttgat tgttactggg
     2221 atatttactg ctgaagaaac agcttggacc atttcgtata ctttctcagg atctaaaagc
     2281 cattttgctc ctgcttctgc tttgattact ttatttactg gacagcccat attgatatct
     2341 attatatcgg cagctgtgtt ttcttcgaca aattgagcag cttcaactag actatcttta
     2401 tttccgccaa atatctgtaa acttaatgga tgttctgttt cttcaatgtg gagcatctct
     2461 aaagtttttt tgtttctgaa atgaatacct tggtcactga tcatttcaca tacgactaag
     2521 cccgcaccaa attctttgac agtgactcga aatgctgaat ttgttattcc agccatcggt
     2581 gcaacaacga cacgattagg gatttccaca ttgccaattt tccatgtgtg ttccacttaa
     2641 atccctccta aggcttcttt cttcaatcca tctaattctt cttcgctata cttgtatttg
     2701 ttattacaga aagaacaaac cgcttctgca ccgtgatctt cgtctatcat tgcttggatt
     2761 tcttctgcac ctaacgcgat aattgcttct gcaaattttt cttttgaaca gtcacatttg
     2821 aattgaacag gcattttttc caaaatgttc aattcttctg taccaagcaa tcctttcaat
     2881 atttcttctg gtgtctgtcc ttcatctaag agggtagaga ttcttggtgt cgtttttaat
     2941 tgttcttcga tcttgctgat tgtttcttca tcggcacctg gcatgatttg gatcatgaat
     3001 cctcccgccg tcttgatgct gtcgtcagta ttgaccaata cactcagtcc aacagctgaa
     3061 gggatttgtt ctgagatagc aagatagtaa gtaaaatctt cagcgatttc tccagaaaca
     3121 atcggcgttt gcccagaaaa aggttctttt aatcctaagt cttttgtgac agtaaacatt
     3181 ccttgtgttc caactgctcc ccgcacatcg attttacctt cactatttag aggaagactg
     3241 agatgcggat ttttgatgta acctttcaca tctccctttc catttgcatc tactacaatt
     3301 cctgttgctg gaccatcgcc ttggattcga acagttagtt tgtcttctcc tttgagcgtc
     3361 gcaccaagta gcagactacc gaccaatgtc cgtcccaaag cagctgagga ggtgtgccaa
     3421 gtatcatgac gtctttgtgc ctccgctacg gtatttgttg cttgaacggc gtatgctcgg
     3481 acgaatccgt cgtgagcaag tgccttaacc aaataatctt tcatttgtta tgtactcctt
     3541 tttccgtttt taaactcata gtgaatcata ctagcaacta ctgctttttt caactttgga
     3601 atgcttcttt taaaaataat tcagaatcat ctagaaaaca ggacatcacc ctatttacaa
     3661 cttaaataaa gaaatctccc gaaaaccggt ttttgttccg gttttcggga gtcatatcag
     3721 atcaattgac ctatattttt ttatttgtat tgatcgtctg aatgattatc ttctgattgt
     3781 tgagaatctg aatcatgttc tgctttattt tcctcattca gttcatgttt tgcttcttca
     3841 aaatcacgtt tttcttccgc ttgtttttca gcgtcttttt cttccaaagc acgttttgct
     3901 tcttcaaaag ttttcgcttc tttttcgctt ggataatcat ttgactcagt accttcaggc
     3961 atcacaccgt gttcaaacaa tgacttgatt gctttggcat ccaatgtttc gaattccaaa
     4021 agtttttcag caatcagttt atgctgtgca cgatgagctt cgatgatttc tcgagcttta
     4081 tcatgagctt ccatcaagat ttttcgtact tcttgatcaa tctcgaaagc gacttgttcc
     4141 gagtaagctt ttgtttgacc atagtcgcgg ccaacaaaca cttgatggtt tccttcatat
     4201 tgaacaggac ctaaacgatc actcattcca tattctgtta ccatgcttcg tgctagagct
     4261 gtcgcttgct caaagtcatt ggaagcacca gttgattgga cattgaagat gatttcttca
     4321 gctgtacgtc caccaagtaa gcctacgatt tgttcaaaca tatcttcttt cgtcatcaag
     4381 aactgatctt cttttggtaa agcgatcata tatccgccgg cgcgtccgcg agggatgatc
     4441 gttactttgt ggacgacccg agcgcgactc aatactaatc cgacaatcgt atgaccagct
     4501 tcgtgataag caaccatttc gcgttctttt ttgctgatta cgcgatcttt tttagctgga
     4561 cctgcaatca cgcgatcttc cgcttcatcg atatcagaag catcgatttt ctttttattt
     4621 cttctagcag ctactaacgc tgcttcattc aagacatttt ctaaatctgc tccggcaaaa
     4681 cctggtgtct gttgagccac aactttcaag tcaacgtcat ctgccatcgg tttattccgt
     4741 gcatggactc gcaagattgc ttcacgacct tttacatctg gacgtccaac taaaatctgg
     4801 cggtcaaaac ggcctggacg tagtaacgct gggtctagta catcagaacg gttggttgca
     4861 gcaatgacga ttactccttc atttccgtcg aagccatcca tttctacaag caattggttc
     4921 aatgtttgtt cgcgttcatc atgtccgccg cccataccgg caccacgttg acgacctacc
     4981 gcatcaattt catcaatgaa aatgatagct ggtgcgtttt tcttcgctgt ttcaaacaaa
     5041 tcacgaacac gacttgctcc aacaccgaca aacatttcta cgaaatcaga acccgagatc
     5101 gagtaaaacg gtactccagc ttctccggca acagcttttg ctaataaggt tttacctgtt
     5161 cctggaggtc cttctaaaag tacacctgcc gggatacggg cacctaattc aacaaatcgg
     5221 cgaggatctt tcagaaattc aacaacttca actagttctt gtttttcttc ttccgcaccc
     5281 gctacatcag aaaaacgtac gcgattagct tttttgtctg cttctttggc ttttgattta
     5341 ccaaagttca tgacacggcc gccaccgcca ccgccgccgc cttgttgtcc catcatcatg
     5401 taaaagaaaa agatgatgat tacgattggc aagaagctaa gcaataggga aacccatata
     5461 ccgctgcttg attcttcttt tactgttgta gcaactttgt tttcttttgc cagttctgtt
     5521 acttggctca atgttgtatc atttggcaaa atgattgttg tgaaatgggt agtagtcgta
     5581 tctgttgaac ctaagatagc taacccaccg ctgttagcga cggtctgctg ttctttgtat
     5641 tcaccagtga tcttatatac accatttgca ggctgcactt caaattcttt gatcttgcct
     5701 gcttgtagtt gttctgtgaa ttttgagtac tcgatatctg acgatcgctg gccgttatta
     5761 ccaaaaatga aataaacgac cattaccatc gctaagatta cgaggacata atacaaggca
     5821 tttttcatca tgccgttgtt ctttttgttc atagttgccc tccctaagcg tcaatttaat
     5881 ttaacagttt tttatcaatc ctgaccattc tattgtacca taatttaatt gagatacagc
     5941 attaatttga ttgataaaca ctaggtttca atacaccgat gtacggaagg ttacgatatg
     6001 cttctgcata atccaagcca taaccgacca caaattcatt tggtacgttg aagccaacat
     6061 aatctgcttc gatatttact actcttcctt caggtttgtc aagtagtgta acaattttga
     6121 ctgattttgc tttgcggtat ttgaaaagat ccactaaata agcaagtgtt cttccgctgt
     6181 caatgatatc ttcaacgatc aacagatcgc gaccttctac attcgtatct aagtctttga
     6241 cgatttttac ttcccctgac gagactgttg cattcccgta gcttgaaaca tccatgaaat
     6301 ccaattccaa atacgtatcg atagaacgaa cgatatctgc catgaatggt accgctcctt
     6361 ttaagacacc tactactagg ggattggtgt tttggtaaat ttctgttaac tctttgccta
     6421 gttctttgca acgaacttgt atctcttctt gtgagatcaa aattctctca atatcctttt
     6481 ccaacatcag gttctccttc caattgcttc tttttgatat ttgtaaagaa gtatgtagtg
     6541 tattttatca gtttcttcac gaatactcaa atatgaaaag gcaaaaggta tcaagctata
     6601 tatatttctt ttggcatctg taatgatcca agcatgctgc ctttgtgaat taggtacttt
     6661 ctgatcgata aaatatctag atactttttt tgtgagattc tctgttaatt taatgcggtc
     6721 tcctgctttt cttttttcga catacagttg cacatcagca ggaatccaaa tgtcatgaga
     6781 aaatagagac catgaatctg ttgctttgat gtcccctgca ttttcaggtt tgaccagacc
     6841 aatccattct gtttcattca aatatattcc ttgttctgga atcagcaaca agcgctggtc
     6901 ttctgtctgg ctagttgctt gtttttcttt tttagagagt acaacaaaat gatactctct
     6961 tttcagtatc caattattgc caatactgag ttgccattgg ctttttttct gttgccactg
     7021 gtctaaaatc gactccattt gttgttcttt caaaataatt cctgtttttg agaatgcttt
     7081 gtcgaaaaag gagtaaaaaa aatagtatcg ttcagcatct gtcatttctt caatcatttc
     7141 tgcagaaaat cgaaattgtt cctcccactg ttcgacttcc ttttcgatca actgtcccat
     7201 atatttttga atgacttggt ctgcccattg gatctgttgc gaaaattgct ggaaatgtct
     7261 caacgcctgt gtattttctt gtttcaattg aggaactacc aaatgacgaa gccgatttct
     7321 ctggatatct aacaattggt ttgtctgatc ctcaaagtaa acaagctgcg attccgctgc
     7381 ataagtatat aggtcttctt tgctaaaact cagcaacgga cgaatcagtc tgcccgtagc
     7441 aaatggttgt acttctttga ttccagaaaa agtatttaac tgtcccccac gaacgatttt
     7501 cattaacacc gtctcgattt gatcatctcc gtgatgagca gtcatcaacg tgtcatattg
     7561 tccttcttcc atcacgcgag tgaattcttg ataacgaaag ttacgagctt ctgtttctat
     7621 ggcggatttg gctggatctt cccaaatact tacatataaa gggagattct tctcttcaca
     7681 atatgagcga agataggcag cttcttctgc tgattcttct cttaattggt gattgatgtg
     7741 gacgatacct aaagaaaaac cgatttggcg ttgcacttgt aaaagtaaat ggagcaaaac
     7801 catcgaatca acaccacctg ataccgctgc taaaattctc gatccttgtt gccagaatcc
     7861 ttgttcattc ccgtgccgaa taaatgactg ctgtaatagc ggtgcttttt tcccgccttt
     7921 ttcaacgttc atcctttcca caccctttct tcctaaaaaa aagagagcgg gaaaacgtct
     7981 aaaacgacaa ttaacctgcc ctctgttgat actatgaccc aaatagtcgc actatcatta
     8041 tttgcggctt tttagctgcg acgtccgcca cggccaccgc gctttccttc tgtgtttcgc
     8101 ttcaatgaag ataagcggtc atcgctatct ttcaaaaatg aactcatcaa tgaatcgaag
     8161 tcttcttttt gagctgaagg ttgactgcga gaaaaagatt ttttctgtgc tggacgattt
     8221 cggttgtcac ggggatcttt ttgttgatat tttttaggac ggctttcttc tccagattca
     8281 gcaggttgtt cctgcgcttt ccggatagat agtccgattt ttccatcatc atttactgaa
     8341 ataactttaa ctgttacttc atctcctaca cttaaaacat catggatgtc ttttacatag
     8401 ccatttgaca cttcgctaat atgaactaga cccgtttttc cagcacctag atcaataaac
     8461 gcaccaaaat ttgtgattcc tgatactttt ccttgcagtt ttgctcctac ctcgattgac
     8521 ataaaaaaaa tgttcctcct aattatttcc cttttttata ttcctgctta ttttgcagtt
     8581 gtagaactag ttgattgatc tgtttcattt gtcgagctgt ctgtatttga ttctaatata
     8641 ggataaactt tttcaccgtc ttttgagtaa aagaatttgc ttctggcaag tttagctaca
     8701 tactcatcat ctttcagcaa tgaaacatct tggtgcagct gatcaacgtc tgcgttgact
     8761 tctttcattt cagtatttgc ttcgactctc aattcgttca attgatgcat cctttgcatg
     8821 tcatggaaaa tctgcacacc tattatcgcg aaaatgacca aagcaccaac aaaaattgca
     8881 gccagtctgc gtcttcgaaa aatcagttga cgttgttgtt gctgaaattc agcatacttt
     8941 ttctttgcat attccgtatc tagcgcagcg attttattgc tttttttcgt cacttggatt
     9001 cgctccaatc tttttgattg tctttttcat tataccaata tcgtgaaagc ttgtctaatc
     9061 tatttctcta gtgtttctgt gcgattttca gaaataattt cgtacatttt ttgtgcatct
     9121 tcttttttgg ttgattcatg taattccgta attttcactt ccaacgtttt gttgccaaac
     9181 tggactttca aaatatcgcc tactttgaca tcactggaag atttcgccaa tttaccgttg
     9241 atttgaattc ttcctttatc agctacttct tttgctactg atcgtctttt gatgattcgg
     9301 gaaattttta aaaatttatc taatcgcata tcttattcct cactttttaa tttttttatt
     9361 taatcgcagg attttcttac caaaaggcaa cattagccat tctcgtactg taaacagacg
     9421 aagacgaatc actgcataaa tgaacacaga agccccgatc ccaactccta tcacgctgac
     9481 aaaaaaagca gtggaacgat gagttacagg accaaatact atattcaata caccataata
     9541 cagcagcagt actactgtca tacaaagaag gctgaacatc aacttccgtc caaaatgccg
     9601 ttctttccaa aaagcattta gctcttttgt ttcaattcga acaaaatacc aaagcgtcac
     9661 acctaatcct aaaagggtag acaggcttgc acctgctgtt cctaataaac cggtcagaaa
     9721 agaagtagtc aatcctttta ccaatagtcc ccatcctgcg ccttttaaag aagggcgaaa
     9781 cgagttcttg ctttgagcaa tactttgata agcttggatg actgcagtga aagcaatgga
     9841 aaaaacaaat aatacaagcg ctgcatttcc cgcataatct ttaaacaacg cataattgat
     9901 ataagggagc agcaaggcta atcccaaaga cgcagctaat gctaaagcgg tcgtcaatct
     9961 aagatagatt ttggctgtct gtaaaaactg acgatagatc cggttggtca aatgacgagt
    10021 caacgcaggc aggaatgtcg cacttaatgc agtggcaatc actaaaccta attgtactaa
    10081 aggctgacct cgatcataga ccccttttgc tatcttcgca ccgtattctg acagtccgta
    10141 aacttccaaa gcatttttta caaagaaaga gtcgatcagc tggaaaaaga taagcaatcc
    10201 actataaatc gaaaccagtc cgccttctac cagaaaacgc cggatcaagg gctttgacgg
    10261 ctgtttgaac aatggaaacc gtcggaagct taacgctcct ccatgaatct tttgttcata
    10321 gtagtgaagg atcccatatg cgcataaacc gccaaaaaca gctccactca ttgctgccgt
    10381 ccctgtttgg tacaccgtcc atccaagctt tctaaaagca aacgcactac agacaatcac
    10441 aaaaacacga acgaactgct caactacttg agaaacagca gttggttcca tcaagaatct
    10501 tccttggaaa ttccctcgat aaaaagatag accaggcatc agcaaaaagg taaaggaaac
    10561 tacttggatc agcggtttca attgtatgtc tcccatcatc caagcaatcc agctgctaaa
    10621 tgcaaagaca aacaaccaca aaaagacacc tgtccaaaat actaagggat aaagattttg
    10681 caaagcttgc tttcgctcga aaggattctt tctttctgca acgtactttg atatgaactg
    10741 cggaagaccg gataaagcta aagtcattgc tatcccataa attggataaa cttgctgata
    10801 aacataaaaa ccttcgtcgc cgactaagtt ttggagggga attcggtaaa gggcacttaa
    10861 tacttttgct atgaaagaag caatcgtcaa tacaaatgct ccttgcatga cgcgccgcat
    10921 ttctttatga tccaaatcat tgctccttcc tacgctgaca gatatttttg ctcacgcaac
    10981 gctttgacaa acttttggat ttcctgaagc catactgctt cttccatttg gttggggatc
    11041 gtcaagcgta tcaccatctg ttctttttca acgcctagac tggcttttag ttttgtcgct
    11101 gtcagtgctt cgaataactg ttccacacta tagcgttttg ttccaacttt acttagagta
    11161 aagactatat gcgcctcctg tttccttatc gtttctagga gcgctcgatc gccatccatt
    11221 ttgatctgcc caattgtcaa gagatgtgct acttcttctg gatattctcc aaaacggtcg
    11281 agcagatcat cttctagttc atctagcatt tcttgcgaat caagttcacg aattcgttta
    11341 tagatctcga ttttctgccg ttgatctgcc acgtatgttt caggtagata cgcatcgaca
    11401 ccgaggtcga tctcaacagt ggtcttctcc acttgactat tctttccttg tttacgagaa
    11461 acagcttcgc ttagcatttg agaatacata tcgaaaccga ctgcatcaat aaatccatgc
    11521 tgctgagcac ctaagagatt acctgcaccg cgaatggaaa gatcccgcat cgcaatcttg
    11581 aatccagatc ccagttctgt aaaatctttg attgcctgaa gacgtttttc actgacttcg
    11641 ttaaggatct tctgctgctc atacataaag taagcatatg cgacacggct gcttcttcct
    11701 acccgccctc gcaattggta taacgtggat aatcccatat aatcagcgtt ctcaacgaac
    11761 aacgtatttg cgttcgggat atctactcct gtttcaatga tggttgtagt gacaagaaca
    11821 tcatattgac cttcaataaa ttcaaaaagc gtattttcta attgcgcctc tgtcatttgc
    11881 ccatgagcat aaccgatccg tgcttccgga acgagttctt ggatttcttc tactttttgt
    11941 tcgatcgttt ctacccgatt atataaatag aatacttgtc cacctcgtcc catttctcga
    12001 tggattgctt cgcgaatagc cccaggattt ttttccataa catacgtttg gatcggataa
    12061 cgattcgcag gcggtgtttc gattactgaa agatcccgca cgcctaacat cgacatatgc
    12121 aaagttctcg gaatcggtgt tgcagtcaga gtcaaaacat ccacttgtgc acgtaactgt
    12181 ttcaatcgtt ccttatgttt tacaccaaaa cgttgttctt cgtcaatgat cagtagaccc
    12241 aaatcgctaa attcaatatc ttttgacaaa attcggtggg ttcctacaac aatatcaact
    12301 tgacctgtac gcagttgttc gatggtttct ttttgttgct ttcttgttct gaaacgactt
    12361 aatagcccga tatttacggg gaaaccttca aatctttcca gcatcgtttc atagtgctgc
    12421 tgtgccaaga tcgttgttgg aactaaaaag gctacttgtt tgctttcctt tatcgcttta
    12481 aaggccgctc gaagcgctac ttctgttttc ccataaccaa catcacctac gagtagtcga
    12541 tccattggtt tttctttttc catatcccgt ttgatctccg ctgcacttct caactggtca
    12601 tcagtttcag agtaggggaa ggcattttcg aattcttttt ggtaggcgtc atcaggtcca
    12661 aaagcatatc ctttttctga ctctcttgcg gcatataatt tgattagatc gtcagcgata
    12721 tcctcgattt ttgaagagac ttttcgcttg gttttcgtcc attcacttcc gcctagctta
    12781 ttgatgcgag gcgctttggc ttcagaggcg acatattttt ggatcagatt cagttgagta
    12841 acaggaataa acagtttgtc gtcgttttga tacaaaatag tcatatagtc ttggtgaacg
    12901 ccatctactt ctagcgtttc catcccaatg tatttcccga taccatgatt ggcatggaca
    12961 acgtaatctc ctgcttttaa ctcattatag cttttcaatc gctctgcgtt tgttactgtt
    13021 tgacgtctcg tttgtttttt cgtcgttttt tggaaaattt ctttttctgt gatagctact
    13081 attttttctt gaggaagttc aaaaccagcc tgcaaagctc cttcaactaa ttgtacctgt
    13141 cctctgatca atgcattttt ttctgtttct actactaata tgtcttcatc tcgaagcatt
    13201 tgttctgctt tgcgtattct gtcctttgtc ggaataaaga tcacaacagt ctgtttttgc
    13261 ttgcgccaac ggtccatttc tgttttcagc aatggcattt gtccgaagaa ctgttgcatc
    13321 gtccgatatt gaaaattatg gattgcttgg aaacgaagat ttcccatccc tttttggaag
    13381 agtgagaaaa aggttgtggt gaacttttcc ttctgcacga ttttttgtat atctgctgaa
    13441 aatgtctgct cagaaaacac ccgcaattcg ctgatttttt gagtctgcca ttctgccgct
    13501 tccctttcga tttcccgatt tgtttccatg attcgctgat aatcatcaac gactagtaaa
    13561 ctatttgaag ggaaataatc caagatcgtc acagattctt ggtaaagaag atctgagtag
    13621 aattttgctt cttctgtcgg gacacctgct tgccagcttg aagtcagctg accgaagtaa
    13681 tcttgcaaaa actcgcggtc tgcttcttct gtggtcgcag caagccgttt ttctagcatt
    13741 ttttgcattt gctctattcc atgttctaga tcctctggtc cgtagacttg ttcactcgtt
    13801 ggggaaaccc agatttcttc cgttttttcg attgagcgtt gtgtttctgc ttcaaaatac
    13861 cgcatagaat ctatctctac atcaaatagt tctacacgaa caggataggt tgtcgttaac
    13921 gggtaaatat cgataatgct tcctcgaata ctgaattctc ccggttttgc gaccatctct
    13981 tctcgtacat agcccatcaa agtcaattgg cgaggaagat tttccacgtc tatctcatca
    14041 ccaatatgcc aattcaattg cgcctgtagc caagttttct tattcggcag tcgtttgtgc
    14101 aaagatgcga ctggcaacaa atagatacct gctttttttt cttgaagtgc gttaagcgtt
    14161 gcaactctct cgcctcgcgc ttctggagaa gaaaaggcca tttcagcaga aagcacttca
    14221 tccactggaa ataaatggat ctgatcttgc ggaatcacat gttgcagatc ttctgctaac
    14281 tgattcgcat aatacaaatt tggcatcaga ataatcatgt ttttatttgt tttcttaaac
    14341 gctcgagcaa atagaagtgt tttcgctgag cccgctaatc ctgtgattag ttgtctaggc
    14401 attttttctt ctaaattgct gacccattct tttacttgcg gatcttttcc gactaattga
    14461 ataatatcca ttccttttcc tccttcctca attgaatcga ttcatcgcat ccacgaacga
    14521 atgcccttct atgagatatt cgactgcttt aacagattga cacatgcttt cttctatcaa
    14581 aggctgattt tctttgctga aaggactaag cacatgctgg actactgttt ttttcccttc
    14641 tggcctgccg attcccacct tgattcgatc aaagacattc gtattcaaat gggaaataat
    14701 acttttgata ccgttatgcc cgccggcact tcccttttga cggagccgga tttttcccac
    14761 tgccagatcc aaatcatcgt agattacgac taattcctct ggataaatcc caaaataagt
    14821 cattagtggg ccgactgcac gaccagattc attcatgaat gtttgtggtt tgactaaaag
    14881 gatcttttct ccattatgaa aaaattcggc aacttctgct tcgaacggat tttttttgaa
    14941 agtagcttga tgctcgcgtg caattttatc cacgaccata aagccgatat tgtgttttgt
    15001 atattggtat ttggtacctg ggtttcctaa cccgacgatc attttcattt ttgttttcca
    15061 cctaattcta aaaagcagca caaaaggctg gactgctaat tagcggtcca gtccatcgag
    15121 cttttttctt ttttacactt gtcaatgcat agtataacac aaaacaaggc gtttcggcta
    15181 aaagagaatc gttaataaat gataaagaaa ttaaaaattt cttcaaagtt taatgaaata
    15241 attgatatat aaaaatagtg aaataacgca catgtaagct tttacatata atacaacctt
    15301 gttatctgtt atattttata gccaaatagc gtgacatggg gcttgagaaa tgttagcata
    15361 agcatgggaa aaaacaaatc aatggaagga ttgatactaa atgactgcag ctgcagaaaa
    15421 aaaagatcat caaaaagtta ttcttgttgg agacggcgcc gtaggttcta gctatgcctt
    15481 tgctctagtt actcaaaata tcgctcaaga agtaggtatt gtggatatca atactgctaa
    15541 aactgaagga gacgcaattg acttgtctca cgctttggca tttacttcac ctaaaaaaat
    15601 ctattctgct acttacgcag acgcacatga tgctgactta gttgttatta cagctggcgc
    15661 accacaaaaa ccaggtgaaa ctcgtttaga cttagtaaac aaaaacctaa aaatcaaccg
    15721 cgatgttgtc acacaaatcg ttgaatcagg atttaacggt atctttttag ttgccgctaa
    15781 cccagtagat attttgactt actcaacttg gaaattctca ggtttcccta aagaacgcgt
    15841 aatcggttca ggtacctctc ttgactcagc tcgtttccgt caagcacttg cggaattagt
    15901 tgacgttgat gcacgtaacg tccatgctta catcttaggt gaacacggag attcagaatt
    15961 cccagtttgg tcacatgcaa atgttgccgg cttgcaaatc tatgaatggg taaaaaacaa
    16021 cccagaaatc gatgaagaag ctatggttaa cctattcttt ggcgtacgtg acgctgctta
    16081 cacaattatc gagaaaaaag gcgctacttt ctatggtatc gcagtagcat tagcacgtat
    16141 cactcgtgcc atcttagatg atgaaaatgc tgttcttcct ttatcagtat tcatgaatgg
    16201 cgaatatgga ttgaacgaca tctacattgg tgcacctgct gttatcaacc gtcaaggtat
    16261 ccaaaaagta atcgaaattc ctttaagtga cagcgagcaa gaccgtatgg ctgcttctgc
    16321 taagcaatta aaagaaattc tagatgaagc ttttgctaaa ctagacgctg aataatcttt
    16381 gaacatgacg gaaaacatct attcggtgtt ttccgttttt ttattttatc acccttatgg
    16441 tttctaaatt ttttcataaa gtagagataa ccttcgtctt ttcctctaaa ttatgctatc
    16501 atgagaccgt tccaccctaa acttttactt aaggagttgt cgatatgaca agatcttcgc
    16561 atcgaaaaaa atcgaaacat gttgctttgc tttcagttct tggtgttctc ttagtgctga
    16621 tcagctttta tacctatcgc agtacatact acacaaagca ttttttgccg aatacagaaa
    16681 taaatggaat aaatgtcagc aatctaacag tcgaaaaagc aaatgaaaaa ttaaaagaag
    16741 cttattctga taaaaaactg gctattaaag aagatggaaa agtctggcag gaagtaccaa
    16801 aatccgagct cggctacaaa gatgatttca cttcggagct ttctccgatc ctaaatgaac
    16861 aaaacggatg gacttgggga atgacttatg tctcagcggc agaaaaacaa gaaatagatc
    16921 ctgtcagtca aaaccaccaa aaactagaca ctaccgttca gacattgaca actaagctga
    16981 ctgatttgaa caaagaccgg acacctacta ctgatgcaac gatcgaaaaa gcaggtgact
    17041 ctttcgttat caagcctgaa gtcaatggaa atacgatcga cgtcgatgct gcggtcaaac
    17101 aattaaaaag tgcggtaaac tctggtaaag atacaattga attgactgaa ttcaaagaaa
    17161 aaccaaaagt aacatctgaa gacagctcac tgaaagaaca actcgcatcc atgaatgcta
    17221 tcgcaaatgt aaaagctact tacagtataa acggtgagac tttccagatc ccatcaagtg
    17281 atattatgag ttggctgacc tacaatgatg gaaaagtcga tttagataca gaacaagtac
    17341 gtcaatatgt aacagattta ggcacaaagt ataacacaag tacgaatgac acaaaattta
    17401 aaagtacaaa aagaggagaa gttacggtcc cagttggcac ttacagttgg acgatccaaa
    17461 cagacagtga gacagaagca ttgaaaaaag cgattctggc aggccaagat tttactcgtt
    17521 cacccattgt gcaaggtggc acaaccgctg atcatccatt gatcgaagat acgtatatcg
    17581 aagttgatct ggagaaccag catatgtggt attacaaaga cggaaaagtg gcccttgaaa
    17641 cagatatcgt ttccggaaag ccaactacac ctacacctgc tggagtcttc tatgtttgga
    17701 ataaagaaga agatgcaact ttaaaaggaa caaatgacga tggaacacct tacgagagtc
    17761 cggtaaacta ctggatgccg attgactgga caggtgttgg tatccacgat tcagactggc
    17821 aacctgaata cggtggagac ttatggaaaa cccgcggttc acacggctgc atcaacaccc
    17881 caccaagtgt aatgaaggaa ttattcggga tggtcgaaaa aggaacgcct gtattggtct
    17941 tctaacaaaa ataatatata aaaagaaagc acccgtacga ttggtacagg tgctttcttt
    18001 ttattatcta acaatgtgct acttacattt cagcagcagt aaagacttct gaaacatcat
    18061 catcgtcttc tagcttatct actagacgtt ccaattgctc tttttgttcc tcatttaaat
    18121 caatcgtcgt ttgtggaatc atcgtcaatt ctgcttgcgc caaagtgaac tctttttcca
    18181 actcatctcg tacagcagca aaatcttctg gtgcagtata aatctcgaat acttcaggag
    18241 aagtttgcag atcttctgcc cccgcctcta atacatcctc aaacatcgtc tcttcatcaa
    18301 cagtcaaccc atcgcgttca atagcgatat atcctttgcg gtcaaacaag taactgactg
    18361 aacccgtttc tcccagtgat ccaccgtttc gagtaaaagc tactcggaca tttgtcgctg
    18421 tccgattacg gttatctgtc aatgcatgga ctaagattcc tactccaccc ggaccatatc
    18481 cttcatacgt gatttcatcg taattcgttt catctacggc actcgtagcc tttttaatcg
    18541 cccgttccac attgtcgtta ggcatatttg cagcttttgc tttgtccaca acaagccgca
    18601 aagctggatt catagatgga tcaggaccgc ctgctttagc tgccatataa atttcacgag
    18661 aaagtttctg aaagactttc cctcgttttg catcttgggc attttttctt ccttggatat
    18721 tatgccattt actatgtcct gacatgattt ttcctccctt gttcttttga gaatacactt
    18781 ctattctagt actatttcac aaaaaaacaa agccacagcc aatctataaa cgcttcttga
    18841 atgccgcaaa aagcacacat aatatcacag cagtcagagc accgatcgaa tccaacatga
    18901 catcttggaa cataggtgtt cgtccaccgg tcatcatctg atggaattcg tccaaacctg
    18961 cgtaccctgt tgctgaaagc caagcaagga tcgctgtcag ccaccaaatt tttagcttgg
    19021 ggaaaacacc taaaaacaag ctgcctccca aaataaaata ggtaccgaaa tgtgcgccct
    19081 tccttaaaaa gaactctaaa aatttggcgt agcccagatg gttgatactc accatacttc
    19141 caccataatt aaaacgtata cccgctaatg tgtctttaaa tggctgatta ggcagccagc
    19201 tttctaatcg cgaaacttgt gattgctgtc cgtaagtttg agaagaactg taaaataaga
    19261 ttataacgac tatcagtgcg attactaaat aaatatttcc gtttttcatt tgtttttttg
    19321 aagaattcat aagtcacctc tgtattttat cttagacttt ttttgaagaa acaacaatca
    19381 ttttcataga gacttcttcc ataaaaaagt gctatactta attttgtatt acaatatgaa
    19441 gggagttttt tcatgagtta ttcattaaat tggaatctag attctatttt ttccggaggc
    19501 agtcattctg atgctttgaa ccaacggatg aagcaattgg aagaccaaat gaacgagtat
    19561 tatcaccgcg taacaaaatg gtcacctagt tctgacaatg cagagcagtt aaacgctatt
    19621 cttcaattgc aagaaacgat tacgaatggt tttacccaat gcagcagcta tattactgca
    19681 ttgctttctg ccaatgtcaa tgattcagat gcaaaggtct tatcaggcaa gctttacgca
    19741 atgctgcctc gtctgcaatc agccgaaact gtattgtcaa agaaatttgc agaaatatct
    19801 gacaatgatt ggaatcagat gctttctcaa tcttcgtttg aaacaattgc ttttcgttta
    19861 aacgagattc gtcgtgatgg ctcacaatta ttatcagaag ctgaagaaaa cattataaat
    19921 acgctttctt tagatggtct aaatgcttgg agcagtcatt atgatacgat tgtagccagc
    19981 atttcgattc ccttcgaaca agatggacaa gtagtcgaat tatctgctgg acaagccttc
    20041 aataagatga tgggcgatcc agatccaaaa gtcagagaaa cacttttcgc agcatgggaa
    20101 gaagcctgga aagaaaaggc acccctcttc acggatactt tgaatcattt agatggtttt
    20161 agactttctg actatgaact tcatggtgtc acagattttc tacaaaaacc attagagtac
    20221 aatcggttga agaaagaaac attgtctgtc atgtgggata caatccagaa aaacaaacaa
    20281 ccccttgtta attacttgac tagaaaagct aatcttttcg gtaaagaaaa aatggaatgg
    20341 caagatcaag atgcacctat tattttaggc gatttgaaag aaaaaacctt tagttttgac
    20401 gaagcagcag catttattat cgaaaacttt aacaaattca gtccaaaaat ggctaaattt
    20461 gcccaatctg cttttgaaaa gagctggatc gaagcagaag atcgtcctgg gaaacgtcct
    20521 ggtggttatt gtactgagtt accagaaaca aaagaatcaa ggatctttat gacctacagt
    20581 aattctgtta atgaggttgc gacacttgct catgaactag gacatgcttt ccatagcagc
    20641 acgatgtggg atttaccttc tttaaatcgc gaatatgcca tgaatgtcgc agaaacagca
    20701 agtacattcg cagaactgat cgtagccgat gctacattaa aagtcgcaaa aacaaaagaa
    20761 gaaaaaatca atttgctaga tacaaaactg caaaatgctt tagctatgtt catgaatatc
    20821 cattcccgat tcatttttga aaatcgattc tatgaagcac ggcaagaagg attagtctca
    20881 gaagataaga tcacagagat gatggttgag gcgcaaaaag aaggctatca aggagcttta
    20941 gctacttacc atccatattt ctgggctgca aaattgcatt tctttattga tgacgtaccg
    21001 ttttataact tcccatacac atttggctac ctctttagtt tgggcatcta tgcttacgcc
    21061 aaccaaaagg gagcaaattt tgaagaagaa tatatccatt tactacgcga tactgcctca
    21121 atgacgacgg aagatcttgc gaagaaacat ttgaatgtcg atcttaccca accagatttt
    21181 tggcaagcag gtatcgacat ggtgactgaa gatatccaaa cgttcatgga attgacagaa
    21241 gaatttatct gatttctatt ttatcacaat aaaaaaagag ttagtccaga atgggctaac
    21301 tctttttgat taatcattct tacatgctga cagcaaagtc aggggcaaac cattgaactg
    21361 aaccaacttt tacgctttcg cctggttgag gagcatggat atattgtcct ccgcctaaag
    21421 caatcgctac gtggtaagtt ccgccttgtg acccccagaa taacaagtct ccagcttttg
    21481 cttgagaaac tgaaattttt gttccagctg attcttgagg aactgtccaa ccaccgatgt
    21541 cacgaccagt tacttgcatg taaacatagc gtgtgaaccc tgagcagtca aagccacttg
    21601 gatctttacc gccccaaaca tatggagtac caatatattt gtaagcttct gctacgattg
    21661 ctgcaccatt cacacttcca gaaggagctg gagcaggtgt tacaggtttt ggttgttcag
    21721 gtgttggtgt tggcgttgga gccggagtac tacttcctgt gccgtttcct gtatcaacac
    21781 tttgatctgt tgatggtgtt gtaggcgcag gtgttgttga ttcttctgtt gttgaagatt
    21841 caggcactgt tgttgattct tctgttgctg aagattcagg cactgttgtt gattcttctg
    21901 ttgctgaaga ttcaggcgct gttgttgatt cttctgttgc tgaagattca ggcgctgttg
    21961 attcttctgt tgaagattca ggtgttgttg attcttctgt tgctgaagat tgtgtagctg
    22021 atgatgattc ttcagtcatt gaagattgtg tagctgatga ttcttctgtt gctgagcttt
    22081 gtgcagtaga tgaagcttga gtagcttgtg cttcagcctc tgcttgttcg cgtgcttctt
    22141 tttcagcttt ttcttgtgca gcttgttgac gagcttgttc tgctagacgt tgttgttcac
    22201 gaatacgagc ttgctctgct tcagcttctg ctttttgacg gttcaagtca gctttcttgt
    22261 cttcagcagt tgcttgttca gcagctaatg aagttttcag tacgttcaaa tcagcttgtt
    22321 tagataaaag atcgcctttt tgagattcca aagcagcttg gttttcagct aattctttta
    22381 gtttagcatc gttttcagct tttttgtctt ctacagcttt tttatcttgt ttttgttgtt
    22441 ccatcaagtc gttgtttgct tttaccatcg ttgtcattgc ttgaacgcgt ccaatagcat
    22501 ctgctaatga atcagcattc aatacagcat cgatgtagtt tgagcttgtg ttgcttactt
    22561 gtgcttcgcg tgcttgtttt tggattgtat cttcacgttt ttcaatacgt tcttgtaaat
    22621 ctgcaatgtc ttttactaat tgtgcagatt cttgacgcaa tgtatcttgt tttgctaata
    22681 ggtcttgtgc ttgtgtgttg atttcagaaa cttggctttc taaggcatcg atttgtgatt
    22741 gtgcatctgc ttgttgattt ttcaagtctg caatcttttg atcttgctgt tgtatctgag
    22801 aatcaaaatc gtccgccgcg gcagcgatcg gtgatgctac ggcagtcaag gtcattgaac
    22861 ataccattac tgctgatatt aaactctttt tcactcttca ttcctccgac tggcttaatt
    22921 taataaatat ctaaatatat ttaagtcttt cttaacgata taaaaattgt accatagcag
    22981 tgtgtcaacg atataatagt tatgttacaa aaatgtttca aaacaacttt taaaaatgac
    23041 aaaactgttc gtttcagaaa atgatcatga aaaagccgcg ttaagtctag catttcttaa
    23101 gataacaacg attctttttc gctttcgacc taaaaataaa aaaagaaatt attcatcact
    23161 gaataatttc ttaaagggaa aaatgaataa tacaaatata atcatgttca acagcagtgt
    23221 aggtcctaaa aaccttgtaa taaaatacgt gttatttact actgctaatt taaaaatcaa
    23281 ctggaccacc atcgtaaaca attcataacc cgtgacaaag atgatcatac tgaaaaattc
    23341 agtaaagatg ttgacatgga taacgtcttt cattacatac atcaaccata caattaaagg
    23401 gagagctact gcatagatac cgataacgcc aatatagtaa gcgtcataga tagccccgag
    23461 aacaatcgtg gtaatcagta aataacgttt ttcaaacgcc atactgcaac ataataatgc
    23521 caatatcaga aagtgggcat ttgacatata ggtgcctttc gaccactccc ctagcattct
    23581 tgttaaatga ccatctatca acatcaaaaa gaacaaaacg attggcagat agtacttcat
    23641 tgtttctttc ttcaacatgg actaatcctc cgctagccgt tggacaatcg tcacaactga
    23701 cacgtcatac atttctgcat aaggtttcac ataaacttcc cgatccagcc cataactatc
    23761 tggtttcgtt ttaataaccg taccgatcgg caagttagct ggagaattcc ctccaagacc
    23821 ggatgtctgg acaacatccc cttctttgat atccgtatca ccagttaatt gggtcaccac
    23881 taaggcatgg agcttttcat catagttttt aagcaaacca aacgcttcgc cattagccga
    23941 agatacccgt actggaaaat gattggagct ttcattagag gatgacagta attcgatttt
    24001 agacgaagcc gtattgacct cgatcactcg gccaatcaat cctttttgcg acatgactgc
    24061 catatttgct tcgataccgt ctttagaacc cttgtctacg atcagcatgt cctgccaact
    24121 gtcaggcgaa cgagtaatga catttgccgt tactttttcg taactagtca gcgtctgatt
    24181 caactcaagt tcttttttca acgcttcgat ttctctctcg taattcttgt tttgctgtga
    24241 aacgtcatca tagctatcga ttttttcttt tagccgctca ttttcggcat aagttgaaaa
    24301 aagattatgg acagaatcta ctccatttcg tacccaccgt actggggcag agattgtttt
    24361 atcaaccatt gcaacactgt cattaacgat ggattggaag aaattgggct ttccttcatt
    24421 cgctcgctgc gcagcggtca cacttagtat cgtcaccacg atgataacaa taaccaaggt
    24481 aataataata tttttattag gattgaactt cttcacaaag acacctcgaa ttaattagac
    24541 tcttgtgttc aattttatca ctaaatgccg tgaaacaaaa ggtaagttgc tcctcttaag
    24601 ctttttttta gcaaataata gattctgtca tttttagcct gaaagacaaa tttttttatt
    24661 tgtttatgga tcaatctttg ctttgaataa ctgcaatcat accatctgaa aaagaaaatt
    24721 ctgcggtatc tgaaagaaat tcgttacttg cataagatgt cggatattct acttctttat
    24781 tttccaatac atacttgctg ccttttaatg taagattcga gacgggagtc aagcagcaat
    24841 aagctaaata cttcatatct ggtatctgtc ttactatatg ctctccaggc aaataataag
    24901 acaaataatt ttgacaatct ctgataataa gctgatgggc aaagggtttg aagcgaggtt
    24961 ccatacctaa ccagagattg gctaaaagat gatccaatct tccgcccgta gctccaatca
    25021 aagtgacttc cgcttgcggg taacgttcaa atgtttttaa gatacctaac tgtgtatctg
    25081 tatcgtcttt ttctgcaggg gaacgggtat actctttggc ttttgcaaat acttccttct
    25141 gctcttcgtt cgacaatgaa tcaaaatcac ccactgccag atctactggg tagcctgctt
    25201 gaagcaggta cagcgcacca cggtctatcc cgacataggc atcgtatttg gacagcagag
    25261 actgcggcca gttttgcgga ttacctccag cagctaataa aattttcatt tagtctaatg
    25321 cctcttttaa tcgttcgatt tgtccgactg gattttcggc tccatagata tatgatcctg
    25381 caacgaagac atccgcccct gcatctttac agatttttgc tgtttctgga acgattccgc
    25441 catctacttc gattgcataa gtccaattgt tctctctacg tagatcagcg agttccgtaa
    25501 ttttttctac tgtttcttca ataaagcttt gtccgccgaa cccaggattg acagtcatga
    25561 ctagtacttg atcagccaaa ggcaaaacat gtttgatcat ggagactggt gttcctggat
    25621 tgataactac tccagcttta actcctaagc tttttgtcat ttgaagtgcc cgatgaatat
    25681 gaggagttgc ttcgacgtgg acagtaatga tatctgctcc agcttttgca aatgctggaa
    25741 tgtagttttc tggatcaacg atcatcagat ggacgtctaa cggtaattta gtaacaggac
    25801 ggattgcctg aacaatattt ggccctaatg tgatatttgg gacaaatcgg ccatccatta
    25861 catctacgtg gatgtaatct gcgcctcctt tttccactaa ctcaatgtct ctttgtaaat
    25921 tggcaaaatc tgcactcaaa atggatggtg ctatcttcat tcttgtttcc tcctattttc
    25981 gtttaccttc tttttttgca tacattggtt ttcttttttc gatttcttgt aagaattgca
    26041 aataattgtc gtaacgtgtc tgtgcgatac cacctgtttc tactctatgt tttacttcgc
    26101 agccaggttc tttagcatgc atgcactctc taaatttgca atggacagaa gcttctacaa
    26161 attcaggaaa ttgtctcggc agttctcctg cttcaattgt caaaaagtca atcgagctaa
    26221 atcctggtgt atctgctact aacccgccat agataggaat cagttccaca tgacgtgttg
    26281 tatgccgtcc tcttccaagt gcatcagaaa tctctcctgt tgctaaattc agttccggtg
    26341 atatttgatt cagtagcgtc gattttcctg ctccagactg acccataaag accgatagcc
    26401 gttctgggaa gtaacggatc agttctgtcg tatcacccgt atgattgggt acaatcaccg
    26461 gatagcctat tttagtatag gtttgcttga tctcttcgat tttttctggg tcgtctgaca
    26521 agtcggtctt tgtcaaaaaa ataatcggtt cgatcgactc gtattccaag gtaactagaa
    26581 agcggtcaag tagattataa gaaaatgcag gctctacgat acttgtgaca atgattccaa
    26641 gatcaacatt tgtgactggt gggcgaacta attgattttt tctcggcaag atttccagaa
    26701 gatagccgtc tgtttgattt tcactctcaa agatcacctg atctccgaca agcggcgtga
    26761 ttttccgatt ccggaaattt cctcttcctc ttgtttgata ggtctctccg tttgcgtata
    26821 catagtaaaa accgctgatt gcttttctga tttgtccttt gataagtgcc acctccgttt
    26881 ttgtttattt taccatacga agaggaaagt aggtgttatt ccatgcaaac ttctcaaagt
    26941 atatggcttg aaaattgatc aaaagagata gaaacacaaa aaagagaatg caacaaatca
    27001 atgtcgcatt cccttctgat atattttatt cagcacttat tttgaacttt ctgaggtgct
    27061 agctgatgaa cttgtggagc tggaagattc ttcttttgaa gagttgcttg tgctactgga
    27121 gctacttgag ctgctagaac tgtctgaaga gtctttttca gaactagaag acgctggatc
    27181 tggtccttta gagtaaatta cgttcacaac cattcctttg gtaattgtcg ttcctgcccc
    27241 tggtactgta cgaatcactt tatctttatc caccgtgttt gaatattcgg aagtctcatg
    27301 cccgatatat tctgcaccga cactagctaa ataactttgt gcttcgcttt ttgtatagcc
    27361 ggagatatca ttcaatttag gctctgttcc ttggcttacg acaaaattga tgtaagtttc
    27421 ttctgctaca acttcacccg aggctggatc ctgactgatg atcgaacctg atggtacagt
    27481 gtcgctatag tcataagtag cactaatatt attgatattg aatccttgtg taacaagcca
    27541 attacgaaca gtttcgtagt tttctcctac atgactactc aaagtcactg tttctggtcc
    27601 tttgctgact gtgaaagtaa tcgtgtctgt tttaggatcg acttctgtat cttctgatgg
    27661 agattgcgaa atgatcattc cgctttctac ctctgaactg aattcctctt ctttttgaat
    27721 caagtcagca gaaaacccta attctttcaa acttgcttcg gcatcttcat aacttttacc
    27781 actgtaatca tctaatttga tttttttcgt gccgctacta atatacagag tgacttcgcg
    27841 tttttgcttg actgtcgttc ccgctgccgg gtctgtcttc actacattac cttcttcgat
    27901 tttgtcatct gcaatttctt ctgtttttgt tgctacttct aatttcgctt ttgcgagtgt
    27961 ttctctagca gaagcttcag aaagatcaga aacatcagga acaacaactt ctcctcgccc
    28021 gccagaagca aagaatacag ctgttccgat tcctaaaaca gcaagaatag ctaataggat
    28081 cagccaccat tttttctgtc ctttcttttt aggatcaggc agaacttctt cctcctctgg
    28141 taaggtttcg ttttctggct tgtcttttgg cagaggcata gatttgaatg aatcaggcat
    28201 tggtgcatct tccgtaaggg gtgtgataac ttttgtttca ttatccatca catgaggctg
    28261 ccatttttct tcgtttgcac gagctggtga taatactgta gaaagatcat cagacatttc
    28321 ctcggctgac ttataacggt ctgcaggttc tttagccgtt gcccgtaaaa tcacgttttc
    28381 cagtgcttgt ggcacatttg gatcaagtgc ttttatagat ggaagatcat cttgaaaatg
    28441 ttttaaagca attgttacag cagattcacc atcaaacggt acgctacccg tcaacatctc
    28501 ataaaggatg atccctaaag cataaatatc tgactgcttc gttgccatac ttcctctggc
    28561 ctgctcaggg gacagataat gaacagatcc tagcatcgta ttcgtctgcg tgatcgatgt
    28621 ttcagataaa gcgatagcaa tcccgaaatc cgtgattttt acaacacctt cattatcgat
    28681 cagtacgttt tggggtttca aatctcggtg gatgatttga tggttatgag ctaaagaaat
    28741 agcagaaagg atctgctgca taatatttac tgctgtttca taaggaacag gataatgtgt
    28801 ttggatatat cgtttcaagt ccattccttt gacgtattcc atcaccagat actgcatgtt
    28861 gtcttcttca ccaacatcgt aaacactaac aatgtttgga tgaacgagtt ctgttgctgc
    28921 taaagcttct ctctgaaatc gtcgaatcgc agtctggtca ttttgaaagt caaatcgtaa
    28981 gactttaact gctacatcac gatccaagat taaatcgtgc gccaaaaata catttgccat
    29041 accaccgcta ccgatatttc cagtgatttg ataacggcca tttaattttt tgcccaactc
    29101 gatcatacac tgtcctcctt atattcgata agaagaacgg tgatattatc cgctccgcct
    29161 gcttcattgg cacgatcaat cagctcagtt actttatgag acaatgggcc ttcttgattg
    29221 atgatcgttc ctatttcttc ttcagacagc atgttcgtca agccatctga acataacagc
    29281 agtcgatctt ttagttgcca aagatgagta gaaacatcta cttcgactgt tccaggcatg
    29341 ccgacagaac gtgttaaaat atttttcctt ggatggttca ctgccatttc ttcgctgatt
    29401 tctcctgatt tgactagttc attgactaaa gaatgatcct ccgtcaattg catgatttta
    29461 tcttcacgaa tcaaataagc tctgctatcc ccaacattgg caatcgtgaa ccagtcatct
    29521 gacagagcgg ccgctacgat tgttgttccc ataccgttat attccggctg ttcttgtcca
    29581 cgctgataaa tcagcatatt ttcttcttga atcgattgaa tgaaccattg ggcgattttt
    29641 tcatcgtcaa cagcttcttg tttttcccaa gcttcgccta gattcgttac cgccatctga
    29701 ctggcaatgt ctccggcacg atgaccaccc atcccgtctg ctaatacagc aagcttgaga
    29761 cctgaacggc tggtaaatac actggcatag tcttgatttg tgttacgtcg tcttcctaca
    29821 tctgattgat attcaatctg cattttttca cctcattact ttttgcgcaa acaacaaatg
    29881 aaaaatccat ctgtgtaaaa ctgatgcgga tacaaagtca acattttctc ttcgattgat
    29941 gattgtagca taggattggt caaaacatca atcctctcaa aatcagggtg tctttccaag
    30001 aaagcagaaa caacgtcttg gttttcttct tttaagatcg tacaagtgct ataagtcaga
    30061 ataccggaag actttaaagt tggtgcgcag ctttctaaaa tttccaattg gatttttggt
    30121 aaattcgcta attcatccgg ctgtttttta taacgaatat ctggttttcg ccgaagcagc
    30181 ccaagacctg aacaaggcgc gtctactaaa atccgatcaa actgctctgc ttcgaactgt
    30241 ttactgactt ctctagcatc catcttttga gcgtagacag cagaagagac acctagacgt
    30301 gctgcattat cttcaatgag tttgactttg tgttcgtgta catctaaagc gaccacttct
    30361 ccaccgcatg acggatctaa aaaagaagca atatgcgtcg tcttacctcc gggtgctgca
    30421 caggcatcta agacatgatg ttcaggctcg atttgcatag acggcgctac aagcatcgaa
    30481 ctttcatctt ggatcgtcat gacgccatcg tggaacaatg agctagaagc taaatgccct
    30541 tttttcgcta ctataccgta tggactgact tggctttttt cagctgtgat ctcttcttct
    30601 tgcaaaacag tgatggcctg atctctggaa agtcggcgtg tatctactcg ggcactagca
    30661 tggctaggct gatatagcga taaaccgagc ttttcagttt cttcgtatcc gatttgatcc
    30721 accagtcgtt tcgtcatcca tagaggcagg ctgattttga cagaaagtct ttcgatagga
    30781 tcagcaatgc cttgcagatc tggtgctcct tgacgctgat aattcctcaa gacaccattt
    30841 acaaatttcc ctgtcccagg atttcctttt acttttgcga tttcgaccgc ctcgtttaaa
    30901 atagcatgtg cagggacttt atctaaaaat tctaattggt atacagaaag ataaagaagc
    30961 atcttgaccc agtcatccac tttttttgct ttagcgatca atggtgcaac gtaaaattcc
    31021 aatagtcgct gatggctaat cgtaccatag atcaattctg taaataaacc cacatctttt
    31081 tgatttagct cgctttgttt gatcatctca ttcaacaaaa gattcgagta ggcgccaccc
    31141 ttgttcactc gctctaatgt cagtaatgcc atatatcgta ctgatttttt cattttttta
    31201 gggatttttt tcattatttc acctgctgac caatctgaac ttgctgaccg cttccgttaa
    31261 ggaaatcatg tacggcctgt tttcctttac cagctggctg taattcattg atggctaaga
    31321 cggtctgttt accgcaagca atccatagat tttttttgtc tttccggatg atcgtaccag
    31381 gtgctttgtc tgtagtttct gccaatactt caaccgataa taatttccag cgtatgccct
    31441 cataggtcgt gaatgcaatt ggccaaggac gcattccgcg tacttggttg tcaatttctt
    31501 ctgcagtttt atgccaatcg attgcttctt gttcccgtgt gatgtttggc gaaaaagttg
    31561 cttctgactc gtcttgaggt actggttttt gtccatccaa aatattgggc aatgtttcta
    31621 gcaatagctt tcttcctaaa aggctaagct tttcaaacat cgtccctaca tcatcttgtt
    31681 tagtgattgg tatgctttct tgcgcataga ttccgcctgc atccattttt ttgatcattt
    31741 ccatgatcgt tacaccagtt tctttctccc cattcatgat ggcataatga acaggcgcac
    31801 ctccccgata tttaggaagt aaagaagcat ggacattgat tgcccctaat tttggtactt
    31861 gcaataactt ttcaggcaag aattggccaa atgccgcagt gacgatcaca tcaggcacca
    31921 attcaatgat ttcttccatt tctggcgaac cggagatttt ctctggctgc agcacacgga
    31981 taccgtgctt cagagccgct ttttttaccg gtgtcggcgt aattactcgt ttccgcccaa
    32041 ctggacggtc gggctgtgtt accactgcct ggatctcgta acctgattct atcagccctt
    32101 caagaatcgg cacagaaaaa gcaggtgtgc ccataaatac tagcttagtc atcaagatgt
    32161 tcctccatat attcttcaag ttcttcttcc gggatctgct gtatcatttt ctcaatgaac
    32221 aagataccgt ctaagtgatc gatttcatgc tggaaggcac gggctaaata gccaaaagcc
    32281 gtcacttcta tctcttctcc gtctcgatca taataacgca cggtcacttc atctgctcgc
    32341 ttgaccgttc catagacatg cggaatactg agacaacctt ctacgtcaag gctttcacct
    32401 tttgcttcga tgatttctgg gttaatcagt tcaaatttct cgccttcatc tacttcgatc
    32461 acagcaattc tcttgttttg tcctacttga ggagcagcga tccctattcc gtcattcgca
    32521 atcattgtct catataaatt gtctaataaa gcgattgtct cgtccgtgat tacatcgatt
    32581 ggctgagcag ttctttttaa tttatcatta gggtggatca atataggata gcgcataatt
    32641 tggccctttc ttaaataaag tttaatggtt cgttgtcaat tgccacaaaa agaccttgtc
    32701 gctgttcttt ctggcttcca ttcaaaattt catccaaaac atgaggaaga ttcggttcat
    32761 gtttatattt gatgattagt tgataataat atcggttttt cactctcaaa atcatgctgg
    32821 gcgttggccc taatagcaag ctttgagggc ttaacacttc ttttagctga ttggcaatct
    32881 gaaagatttt ctttgccgct acttgttctt caggatggct ggcggttatt ttcacagtaa
    32941 agtaataagg aggatagcct ccacgatgcc gtacatgcat ttcctgctga taaaactgct
    33001 cataattttg ttttttagcc aattctatgg cgtaatgttg aggattgaat gtttggatca
    33061 cgacttcgcc tgctttttcc gctcgccctg ctcgtcctga aacttgggta agcaattgga
    33121 atgtatgctc actagaacga aaatccggta aattcaggga ggtatcagca ttcaacaccc
    33181 caactaatgt cacttctgga aagtccaaac ctttagcaat catttgtgtc cctaataata
    33241 tatctgcttc ttttgcaccg aatttttgta aaatctgttc gtgtgcccct tttcttcgag
    33301 tggtatctac atccatacgc aaaatcctag cttgcggata aagttctcgg agttcctctt
    33361 ctactttttg agtacctgtt ccatagtagc ggattttatt gccgccacaa ttaggacagc
    33421 gattgggaat aggttcttca tgtccgcaat aatggcagcg cattgaacgt gtatccatat
    33481 gcagtgtaag agagatatcg caattaggac atggaagaac aaatccacag tccctacaca
    33541 tcacaaaaga cgaatatcct cgacgattga gcagtaggac agtttgttct tttttgtcta
    33601 atcgattttg tattttttcc tgcaaattag cagaaaaagt agaggtccga tgattctgaa
    33661 actcttcacg catatctacg atctcaatag ctggaagctg tgcagctgct ttggctcggt
    33721 ggtttaactg caataactga taaacgcctt tttgagctcg tgctcttgat tctaaagagg
    33781 gcgtcgcgct tcctagaact attgggcaat gatgatattt actgcgccaa attgctagat
    33841 ctctcgcatg ataacgcggt gtctcatctt gtttgtaact tgattcatgt tcttcatcga
    33901 taatgatcag cccgatattt ttcagcgggg caaaaatagc agatcgtgcg cctactacta
    33961 cttgcgcttc ttcccgctca atcttgcgcc attcatcata tttttcacct tgggaaagtc
    34021 cgctatgtaa gaccgcaact gattctccga agcgtccttt gaatcgctcg accatttgcg
    34081 gagtcaatgc gatttcaggc actaacataa tagccgtttt attttctgac aaaacatcag
    34141 cgattgcctg aagatatact tctgttttcc cgctgccagt gattccctcc agcaaaaaga
    34201 cctgatcttt ttgctgctga cctgctgtga tgatttgttc gacagcattt ttttgttctg
    34261 cattcagttc caatgccgtc gtttggtcaa atactcggtc tttataaggg tcgcgataac
    34321 gttctgattc tataaactcc agccagccct tttcttttcc ttgattcaag acagcagtac
    34381 tgattccttt atttttgaaa taagcaattg gttcttcttg gctcagttca gataaacaca
    34441 aaagaagttc tttttgtttc ttggcatttt gccgtaattt tgcccattct tcttcgattt
    34501 gcttttcagt taacaaagaa cggaccaaac gaatcgtttt tactttattt cgtgtatgga
    34561 cttcatattt gatatctact aattgctctt ggcgccagcg aatcaattga gaaaggctgc
    34621 cttcttcttc tgcttccttc caggaaatca cttctcgctg atcaaacaat acctgaactt
    34681 ctggtgtgtc tgccaaagga tagatcaatt tatcatattc tgctttcatc acactcggaa
    34741 gcatcgtttg tagacaagtg attttgaatg cgtaagtaat ttttttcata taatcagcta
    34801 atgaaagtaa ttcttcattg accaccggag caaggtctag caaacgaata actgccttca
    34861 aagatggatt agctgattct tctgattttt ggatagccat aacaaatcct tggacatgac
    34921 gattgccatt accaaaaggg acttctactc gcatcccgac ttgcaaagct gtttccattt
    34981 cagagggaac caaataagtg aatggctgat cggtctgcat agtaggtacg tccacaatga
    35041 cttcagctgt taatgtcatg gttcgtttcc tccttttttt gtcttttctt atcaagcgac
    35101 aaaaagttcc ttgcctattt tactagagat cgatggattt gtcttatctt ctgccaaatc
    35161 ctaacaaaaa attaacagca gataagaaaa aaaagaactg tgtcaaaaag ttcagacaca
    35221 gccttttcta ccagacaata agtggcgaat gtttgaaaag gaaacagctg ggtttgcttt
    35281 tctttttaga tgttcgctct atttttcttt attacatttt ttgctcttca cggatacgtg
    35341 attccaattc gtgttgttca cgctcttttt gtgcttgacg ttcctcttct tccatcttca
    35401 aacgttcacg ttttaattca ggatgtggat cattgactac atttcctgca tcgatttctt
    35461 ctaaagcttg accgacgctt tttactgaat caaaagaatc tagagtaggc tgtgcacttg
    35521 catccaattc atgcgcgcgt ttacttgcta aaatcactag agaatatttt gaatttacac
    35581 gatctaatag tgagtcaatt gaaggtttta acatcataag actacatctc ctttaacatt
    35641 ttaatatatt taccgattac gcgatctact cgaaaatgtt cactggcaat aatgtttttg
    35701 attcgttcaa ctgctagcgg cacttcatca tttacaacag catagtcgta aagagccatc
    35761 atttcgattt cttcttttgc aactttcatt cgtttttcga ttacttcatc tgcatctgtt
    35821 cctcggccta cgatccgtga ttttaattcc gctaaatctg gcggagtcaa aaagatgaat
    35881 acaccatcgg gcactttttc tttgacttgt tttgctcctt ggacttcaat ctccaagaaa
    35941 acatcttttc cttcatccaa tgtcttgttg acatatgaca aaggagttcc ataataattc
    36001 ccgacatatt ctgcgtattc cagcatctct ccagcttcga tcattgattc aaactcttct
    36061 ttgcttcgga agaaataatc aacgccttcg acctcccctt cacgcatttt acgggtagtc
    36121 attgagatcg aatactgaaa atcattatcc tcactctcaa aaattgcttt tcgtactgtg
    36181 ccttttccta caccggatgg accagacaac acgattaata atccacgctc tgacatgacg
    36241 acgtcctttc aactagtctt tttgtactta tcgtttattt tgcacttttt ttctgcaact
    36301 ttcaaggtta tctttaatta tagctagaaa ttggtaaaaa agctaccctg taccaagagg
    36361 tcgtaaaaaa gctgtcctgc atcaagtaat aagaacagcc aaacataaac agcttctcat
    36421 gtttttcgtt tggttggggt tattatcgca gatgcaagct tttgaacgcc gtgtataaga
    36481 ggtcgtgaag aagccgatta gtttcgagta tgaagaaaaa ttttgtgaaa atagtttctc
    36541 acatttttgc ataaatttgg cttaataccg agaaaatggt ttttgaacat cacttattca
    36601 aaataaaaaa agattgtgac acgaattttg tcacaacctt taaatgtatt cctagagcaa
    36661 agaagctaag cgaagaaatc caattctgtt tatttattgt atttctttgt tcagcatcct
    36721 tgctaaaagc ccatattgga caaagtattc actacaccca tgatctcatc ttttcgagaa
    36781 cgtgcttttt ctgtcggatc ttctacctct tctgtgtcaa ttcgaacaat aaatttccga
    36841 ttcatcgtca cgtaagcacc gtcgttgatc aaaataaaca gttcgttctc attatagtct
    36901 gcacaggcat ctttccatac gtcaaagccg tttttttcag aagggatatc ccgtttaacc
    36961 acagtttttc cacttaattc gtggaacgtt acgtcataat atttcattat ctctacctct
    37021 ttcgtcattc aatattttgt acttgttctc tgattttttc taagatcgtc ttagactgga
    37081 cgacttgatt tttaatttcg attgcatttg atttggaacc gatcgtgttg atttcccgat
    37141 tcatttcttg gatcaggaaa tctaattccc gacctacagg acattgtgct tcgataagtt
    37201 catctagttt ttgtacatgg atcgccagac gatccagttc ctcgtgaata tctccgcgtt
    37261 ccaacaagat ggctagctcc gtcaacaacc gttgctcatc gactttcgct ccaagaaatt
    37321 cctccagctt tttttgatac cttatttgaa attccttttc atagatttct acaaaactat
    37381 cgagtttttt tagtacttct ttgaattctt cagtattctt tttcaacact aaagctaaag
    37441 cttgaccttc tttttctcga ctcgcctcaa tagaatcaag tgcgcttttc aatgtatcta
    37501 gagctaactg tgccaattct tccatgctct cttccggttt ttcttctacc gttacgaagt
    37561 tttcgtccag cacgaagcgt tccgccatcc gactaattga cacttgattt tgactaccgt
    37621 atcgttgttg aattccgtct gtcaattgag tgataagtga atcaatcaat tcccagttga
    37681 tgacgatctg tttttcgttt ttgccaatat aagctagatt cacaaatact tcgattcgac
    37741 cgcggcttaa ctgctgttta ataagctgac gaaaatcgtt ttcaacaaag ttcaggattt
    37801 taggacttcg tatttgaaca tctagaaaac gctggttgac actttttatt tcaatatcta
    37861 actgatacgc ttttgtttcg cggatggctt tgccaaaacc agtcatactt ttcattcgac
    37921 agggtccttc ctcattacat attttctatt gctttttgca gttgttcaaa ctcttcgttc
    37981 gtcaatgtca ggcctttccc cattttttca tggtctggcg cccaatctcg aagatcgaat
    38041 tttggcgggc gaccattcca gctgactagg ttcagttctt tacgccaccc cttgttgttt
    38101 tctgacaata tagcgatttc ttctacgatt tcatatgaaa attcttgtgc catggttgtt
    38161 cctccttgag ataatataaa tataggatca gtaatgtgtc aactggttgc ttaagcaatt
    38221 catgctgcaa acgatccttg cgttttcctt gtagcagata atcaaaataa acagttttcg
    38281 tgcgctcatt ttgtattttt tgctggtatt ttcgatacct tcgccattct tcagtactta
    38341 attgaccaat ttgtgccaat aaatcatccg gcatactttt tatatcttgt tggcagatca
    38401 tttgaatcat catgtctact tgatagattt cattgggcag taaaaaatgt ttttttgcta
    38461 aaagttctag catcgccaat ccgcatgtct ctggtacgac cctgttctta tataaattac
    38521 gcagtttttg ccaaaatttt atgcttttga aaaaagataa ttctaactga atttcttttg
    38581 ttaagtaaga caaaaatcct cgccagtatg ttcttgcagg tttaacagaa tgaaatgtat
    38641 accagttccc atagtaatag cctaatcgtt ttttcatttc cagatagcct aattgtaaat
    38701 ttaattggtt cctttggcta tcaaaaagca gaaagctgcc tagtgtccac aatgtttgac
    38761 atttccaatg gataaaaatg tctggcatac gaattttttt cgctgggcct ggcccttgca
    38821 catctacgac aaatgcctct gttgctcctt tgttaatcgc aatatcaata ggtattttgt
    38881 tgcgataccc accgtctgca tatttatttt ttccgatctt tcgataagcc atagcgggat
    38941 aaaatgaagc agaggcgagt atccaatctg gtatttcttc tgtttttgtc tgttggatat
    39001 cagtcactac ttccgctagt tctggcagtt tagtagacac cgtatataaa cgtattttgg
    39061 gattatgttt gagtaaggac aaatccaaat tgtctttgac catcttttcc aatggagcag
    39121 aagaaacccc gccttcaatt agtgctgttt tcgtcatttg gcgtctttct cgatagaagc
    39181 gttcacgaag gtcttcaact gcagccattt ctggtaacgc aagcacttgt cttgtcgaca
    39241 gctttttcca taaattcaca gcttttttca tatctccttg taaaatcaat gcgccgttca
    39301 acgcacctac cgaggtacct gtgataatct caaagttgat atcatgttcc ttcagcgcct
    39361 gccacacacc aatctgataa gcaccgtgcg ctcctcctcc acctaaaaca agtgctgtat
    39421 gatagaccag ctttttattt aattgtgtgg ttccttgata gccgttttta tgaaaaatac
    39481 gcaaccattc ttctgataga ggagattgaa ataaaaaaca acagttttct ttaataaaaa
    39541 aacgagcgca agcttccata taccgaaaaa attttttcca ttcaaaagtt tcaccttcaa
    39601 tcaacagatt attaaaaata acatctcctc tacttttagc aatagtaaaa tagacggtat
    39661 attttttcga tactgcttta aaagcacatt gcttttttgc agaacgccaa aaagcgtctc
    39721 tactttcttg atgactttga aaaaatggaa ggggttgttt tgcttgctgg ataggcgaat
    39781 acagcgacat tttctgtgaa aagtaaactt tttttatctc catcgcgctc tcctttttct
    39841 tcaagtatac cagtacgtca taaaaaggtc atgagtctcg tattcgactt tgtagattcc
    39901 ataagaaaac aattcataaa acctccttta aataaaaaaa tacaaaaatc acaaaaatta
    39961 attgaaaatg agagtgtttt caatgaaaac gtgattttgt aatggaattt taaaataaaa
    40021 gaaacaagct tttttaatgc aaatgttgta aaataaaaga gattaagagg aaataagagg
    40081 aggaacatga tgactgagtc agtttatgta catattgata ccacaagtaa tgctgtttta
    40141 acaaaagggc tgactgctac tgactttgca aacagtatcg tgcattttcc tcaaaacttg
    40201 ctcttgcttg atccatctgc ttctgctgga gaatatgaat cccatacagg gctgaaagta
    40261 atccgtggta cagaaaatat tcagcgtttc ttttcctctt ctcgtaacag gcgtggattc
    40321 gatgaattga agtggatcga ctttactgat ttaacgatgc tgaaagaatt gacaccttta
    40381 gaaatttcag aattattata ttttggtcat atgaaaacac atcttcattc gccgtttttt
    40441 tataaactac agaataattt tgtatacttc gatcttaatg atcagctgaa tcggatttat
    40501 tatcgctatt tagatgaatt ttataggatt tttgctagta aattaagttc gatcttgtca
    40561 gataagatca atgtaaaaaa atcttttttc aaaaaagaaa cacctgttga aaaaatcagt
    40621 gtggaattat tgaaagaaat gcgtaatttg atgcaggaag ggatcatttt ttcttttgat
    40681 caagttggtt tgatcgatgg taaatatcgt atcccaatct atgtgattga agattatatt
    40741 tggaaattaa aaggcacccg gtatcgagaa gaggaaatca tcgctacttt aatctactct
    40801 gtcaccgatc gacactggca attggtagaa gatgccgatc tttcttatct gccattgaag
    40861 tagatctatt tcttatcaaa aaataaaaag gaaacaagaa actaggacat gattctgtct
    40921 caagtttctt gcttcttttt tatttacagc tcctgtcgtt tatctggcac aaaaatcgaa
    40981 ggagtcgttt gttggatccc taataaaacg atgatcgtta caatagctgt actcaacccg
    41041 ctggcaccat tcattacgaa agaaaagagc caaggattca tcccccatag tgcatacgct
    41101 ccccagaaaa ccactcctgc aataaaatgc caaaaatatc tggaaagtgc gccaatcaat
    41161 gcaccaaaca cgatgctttt tctaatttgt ttcgtattat cgtctacata gcctttttgg
    41221 acatttgaag ccatgagccc tgctgcgcct gcaaaagtat aggcaatcgg gtactcaatc
    41281 aacacttgaa cgacacttaa ataataaact tgtcccaacg gaaaatgtag gatgccccaa
    41341 atgaaacctg ccagcatccc tgcctttgtc cctcttctta acgcaaaaag agtcaatgga
    41401 atctgcccta atgaaataga aaaactgctg ccgatctgga tcggaatcaa cgacaacacc
    41461 atcgctaacg ctgcaacaat cgttccttct acccaaactc tattttttcc cataaaaaaa
    41521 cttcctcctt tgaataaaaa agtcacaaag gaggaagcag ctttcgaaaa tagtcaaaca
    41581 gctgtttatt cacaattcct acgctcgcat aaccgataca ggtacgaagg gtttagaaat
    41641 agattcaatc tcagcccaat tggactcccc tttgtgagtt tatttattat ttattttttg
    41701 tttcaaacaa tggactaact ggtttgtttt catggatacg tttgattgca tctcccatta
    41761 gtccgccaac acttacttca tcgattttgt cgattttacg atcttctgga agatagatcg
    41821 agtctgtaac aactagacgc tcgatagctg aatcttctat tctttgtaaa gcagggccgg
    41881 ataatactgg atgtgtacat gaagcataca cactagttgc accagcttct ttcaaagcat
    41941 tggcagccaa tgtgatcgta cctgctgtat cgatcatatc atcgatcagt acacaagttt
    42001 tgcctttgac ttgtccaatg atattcatta cttctgcaac atttgctttt ggacggcgtt
    42061 tgtctatgat cgcaatcgga gatttcaaaa attccgctaa tttacgtgcc cgtgtgacac
    42121 caccatggtc tggtgaaaca acgacaacat catcgccttt gattcctttt tctaagaaat
    42181 aattggcaat aagtggcgca cccatcaaat gatcgactgg aatatcaaag aaaccttgga
    42241 tttggactgc atgaaggtcc aaagtcagca ttcttgttgc accagctttt tgaatcatgt
    42301 ttgctactaa tttcgctgtg attggttccc gtgcacgtgc tttacggtct tgacgcgcat
    42361 agccataata aggcatgact acgttgattg tttttgcact tgctcgtttc agtgcgtcaa
    42421 tcatgatcag caactccatc aagttatcat tgactgggct gctcgttgat tgaataacat
    42481 agacatgcga tccacgaata ctttcttcaa tgtttacttg aatttctcca tcactaaatt
    42541 gagtaacaga tgattttcct aactcgacgc cgactgctgc tgcaattttc tctgctaatg
    42601 ggcgattgga atttaacgca aaaattttta atcttgggtc aaagtaatgt tttgacatgg
    42661 ggacctccac tttctttttc acaactctag aattctacct aataaatagt agtcatattt
    42721 tttaaataaa tcaagtgatt ttgcaaaagt cttagtttag ataagggagt ttttttgcat
    42781 atccttcttt attgacttgt cttgctctcg caattgccag cgcatattct ggaatattgt
    42841 ctgtaattgt tgacccggca gcaatcgaac tgtttttagc tacctcgaca ggtccgatga
    42901 tattcgtgct agaaccaata aagctgtggt cgccgacagt cgtatgatgt ttgttctttc
    42961 catcatagtt gacgaagaca acaccacatc cgacattgat ttcttctccc agcgtagcat
    43021 caccaacata agtcaagtga ccgactttag ttcctttgcc gatttgagca tttttcactt
    43081 ccacaaagtt tccgatatgg acgttttcgc caatttcagc ttttggtcgt aaatgtgcat
    43141 aaggtcccac atctgcatgt ttcttgacat ggctgctttc aatgactgag ttttcaatga
    43201 cgacgtgatc ctcaatccta ctgtctacga tttttgaatg tgacccgatg acacaatcag
    43261 aaccaatgac tgtatttcct tggatttgta cacctgcttc gatcaatgtg tctggaccga
    43321 tttctacgcc tgcatcgatg tatgtcgttg ctgaatcgac aaagcttacg ccattgacca
    43381 tatgcatctg gttaattcgc tttctcatga tctcatttgc tttagctaac gcgatccgat
    43441 cattgactcc cattgattct tcaaaatcat ccgtttgata agcagcaact gtatggcctt
    43501 cttcttttaa gatttcgatg atatctgtta gatagtattc accttgagca ttgtttgttc
    43561 caactttgct taacgcatcg aacaaagctt cgttgtcaaa acagtaagtt cctgtattga
    43621 tttcttgtac aagagcttct tctggtgttg catctttttg ttccacgatt ttttctacga
    43681 tcccaatatg gtcacggatg attcgtccat atcctgttgg gttttctgct tgtgccgtca
    43741 agatcgttgc acttgcattt tttccttggt gatattcaaa aagattgttc agcgtttcag
    43801 tagtcaataa tggtgtatcg ccactgataa ctaacgttgt tccttttttt ccttccaaga
    43861 aagaagcagc ttgcaaaaca gcatgtcctg ttcccaattg ttctgcttgt aacgcatatt
    43921 tactgcgttc tcctagttgt gctttaactt gttctgctcc gtgtccaacg atcgtaatca
    43981 cttcatctgg tttcgtttca ctgacccgat taatgatatg ttctaccata ggtttaccag
    44041 aaacgggatg taaaacttta tataattttg acttcatgcg ggtacctttc cctgccgcca
    44101 aaataattgc ataacgtgcg tccaactgcg tcactcctaa atatagtttg ctgatttcag
    44161 tttatcccag tatacccatt ttttcaacaa aacaagtgat tttcttttat cccagcgttt
    44221 tctggataaa aaaatagata gaccataacg ggcctatcta tttggtaacc ggtgctctcc
    44281 tgccaactcc tcggtcacaa gtcacactac tcataaaaca tgaaaagctg ttttttgaaa
    44341 tcgtttcttg tgacttagag ttaaacggta ggcttacaac ctcttcatta aacgtttttc
    44401 aatcagctga ccttcggtat tagctcaata aacggcaaaa tatgaggagc aatttttccg
    44461 tttattgagc taatactcag gacaggacgc tgatttcaca acctctagat aaacggtgtt
    44521 caaaagccag tttctcggta ttaagccaaa tttatgaaaa aatgtggaaa gccattttca
    44581 caaaattttt cttactactc gaaactaagc ggctttttca caacctctta ttctgaaaaa
    44641 taatttcctg gcacaaccgt aatatttttc gtttgagtat ctacatcttt gacataaagc
    44701 agtgacgtat aatcatcgat ggcgcgatga ccgttgaatt ttgattcggc aaatacagtg
    44761 atgccaacaa gttctgattc gaattcttca atcaagctct tcattccgtt gactgttccg
    44821 ccgcctttca tgaaatcatc cactacaagg acacgtgaac cacgtttcaa acttctctta
    44881 gacagttcca tcttttcaat tctttcagaa gatccagaca catagttgac acttactgtt
    44941 gatccttctg ttatttttga gtcccggcgt acaataacga atggcgcatt cagataatag
    45001 gacacagctt gggcaatagg tacgcctttt gtggctacag tcattacagc atcgattttt
    45061 tcaccaaggt atttagaagc aatgattctt ccaacttgac gcagcagttc aggttctcct
    45121 aacaaatcag agagataaac atatcctcct ggaagcagac ggtcttgttc tgatagacgt
    45181 tctgctagag attcaatata ttttttcgcc tcttcatatg aaatttcagg gatgaacaat
    45241 acaccacctg cagcacccgg gattgtttcc aaggttccat accctcgttc tttaaatgtt
    45301 ttcttgatga ttcccaaatc ttcactgata gaagatttag cagattcata acgttcggca
    45361 aagcttgtta aggaaatcaa ttcatgcggg tgattcaata aatactgtgt catgtcaact
    45421 agacgttcac tgcgacgtac tttcacctat tccaactcct tttaagaaat tttatcgttt
    45481 tacattatat aagtttttct tcaaaaattc gagcatttta tgaaaaaata agaaaatagt
    45541 tcggtattta ttaaaataaa aagaagccta taaaaaattt ttcgttttta taaggcttct
    45601 tttctttata atttagttat tttttcttct gaaggcaacc aatattcgtt tgatgatatt
    45661 tacaatcagg aacaaagcaa taaagactaa tgtgattgtt gctcctggag gtgtatctag
    45721 ataaaacgag ctagttagtc ctgacagcat ccctagaaat cctacacaaa cactgatagc
    45781 aatcacgaca ttgaagcttt tgcctaatcg cataccgatt gctgcgggaa gaaccatgat
    45841 tgcggatatc aaaagtgcac cggcaatcgg gatcatcaca gcaatagcca caccagtaat
    45901 cacattgaac atcatcgaca tcaaatgaac gggtagccca tccacgtggg cggtatcttc
    45961 atcaaaagtc agaacataca tcggccgttt gaataaaaag aacaatatac cgatggcaac
    46021 gaataagatt cctaaaaaga tgacttgctc ccatgtaatg gtcacgatcg aaccaaacag
    46081 ataggattgg atacttgttg ctgaattccc tccgctcaaa ttcatcaata ctaacgctag
    46141 cgccaaacca ccagccatta aaatggcaat ggagatttct gaataggttc ggtagatcgt
    46201 tcgcagatac tccaatatca cagctgctaa tacaacgact agcatggtcg taagtgtagg
    46261 attaaggttc aaaaagaatc ctaacgctac ccctgctaaa gagacatgag aaagcgtatc
    46321 agccatcagc gattgtcttc tgatcactaa aaacacacct agcatcgggg cgattccggc
    46381 aatgaagata gcggcaagaa acgctcttct catgaattca tatgaaagca acgccattga
    46441 gaatcctcct ttctcaccag tcggatctgt ctgtccgcat actgtttgat atcttcatga
    46501 tcatgggtga tcattagaac tgatttccca tggacatgcg cactatgtcg gagaagacga
    46561 taaaattcat ttctggattc ttcatccata cctgttgttg gttcatcaag aacaaataag
    46621 tcagggtcag tagcaaaaat acgcgctaaa ctgattcgct gcttttgtcc gccagacaat
    46681 tcacctattc gccgatttcg catatcccac atccctacag cttctaaagc acgctgtaca
    46741 tgttgatgat ctttttcaga gagacgcttg aaccagcggc ctcttggata tcttcctgaa
    46801 cggactaatt cgatcaccgt actaggaaaa cctgcattga aagatgcaac ttgttgagga
    46861 atataaccga tacttaattt ttttccttct tgatttttct cagcaatctt tatcattcct
    46921 tttgaaggtt tcagtaatcc taacgatgct ttcaccaagg ttgacttggc tgctccattt
    46981 tctccagtta aaatgacaaa ttcacccgca tctacatgat aagagacatc ttctagtaca
    47041 ggttcatcat catagtaaaa ggtcaaatca gatatttcga tataacgcac cttacaacct
    47101 ccattctaaa acttaattga tactttcttt taaagctttc agattttttt ccatgacaga
    47161 aatataattt tcgcctgctt tgatttgttc attagtcaag ctttctaaag gattcaacac
    47221 ttctagcttc actcctgttt cattcgacaa tgtctcagct accttcgaag aggcattttc
    47281 ttcaaaataa atcactttga tgttgttttt cttcacaaat tcttttagct cagctaagcg
    47341 acttggtgtg ggttcctgat ctggtgaaac gcccgaaatc gctacttgtt ttaagtcgta
    47401 ttgattcgct aaataagcaa aagcagcatg ctgagtgaca aatgtgcggt ttgttgcatc
    47461 ttttagctca tcggagtatt tttgatccag tgcttcaagc tttttgatat aagcatcggc
    47521 attcttcgta aaagtttcct ttttatctgg atatttttcg cctaattctt cagcaatcgt
    47581 tctgacttct tttatagcta ataccggatc gagccaaaca tgtggatcgt attcatgtga
    47641 atgaccatgc ccttcttctg attcttcatg ttcgtctgct tcttgcgatt ctaaacggtc
    47701 gattccttta gctgcttcga tcatcaaggt ctgtttgcta tccactgaat cctttaacga
    47761 atccacaaaa aattccatgt ctgaactatt atacacgaaa acatctgcat cggaaatttc
    47821 tgctctttct tttgcacttg gttcgaaatc atgcggctcc gttccagcag ggatcaaaag
    47881 tttcacgttt ccttcatccc ctacgatctc ttttgtaaaa tcatacatcg gataaaaagt
    47941 cgtaacgatc gtcaaatcct cttttttgtc tgcttccttg ttcgtatttc cacatgctgc
    48001 aaacagtaaa gcacctatca ttaccgtagc tgtcaaaaga tattttttca tttatatgct
    48061 cctttcaatg aacaaatcgg aattttacga tttattaacg tgaactaatg tatcaaaata
    48121 aaaaaacaca gtcaatagaa atccatcaat ttctgattta agcatgtata ttcttgatta
    48181 aatcgtaaaa tggatgagtt gctgctcata tctagccatt tcactacaat aaaaaactga
    48241 agaataccat tttaggattc ctcagttttt catcacctat tttacttctt atattttatt
    48301 gcagggttcg tactaaataa acttcctcac aaaaaccttt taacccatta tatactctct
    48361 gcgctcttga ataatgttga cacaaggcaa aaacggttgg tccgcttcca ctcatcaatg
    48421 cagcatctgc tccgtacttg atcatacgct ctttgatttg atggatgact ggatgccgct
    48481 ttcccgttat atcttctaaa ctgtttccca tttttttgat catctgctga taatcttgct
    48541 ctcggatggc ttcagcaagt ccgtggatgt cttgatgttg gatggcttgc gtatctactt
    48601 ctctaaacac tgtacgtgta gagacactca ttcttggttt gaccaacaca acccagcact
    48661 gtggcataga aggtaatact tggatttctt cccctttgcc accgacaaat gccgtacttc
    48721 cgtagacaca gtaaggaaca tctgtaccga cttccaaacc gatttctgcc agttcttcta
    48781 acgaacagcc cagattccat aattggttga tccctcttag agcagctgca cagtctgtac
    48841 ttcctccgcc taatccagct gctactggaa tattcttctt aacaaagatc ttgacaccgg
    48901 ttttgatatt gtatttattt tttagcaata aagcagcttg ataaacattg ttccgttcat
    48961 ctaccggtaa aaatgcttta tttgtttcta caatgatttt gtcttctgtt atttcttcaa
    49021 aaaatagatg atctgccaag tctacgctcg acatcaccat ttccaactca tgatacccat
    49081 cttgccgtct atataataca tccagtccta aatttatctt ggctggcgct ttttcaatca
    49141 attccatcct ctcacccatt cgaaaagtct aagtcgcaag tattctatca tagggaaaaa
    49201 taaaagtata gcttatgtct gcctagtggt tgattttgtc atcgtttcaa cagctttcac
    49261 agaattttct cttcataatc caaacaagtt ctagtgattc ttataattct gtgagcactt
    49321 cttctgccaa actcggaaca tttccttttt cttttcctat cagactgagt agctgcatgg
    49381 tagcaaatcc gaaattactt cctttcctat aagcaaaata acgtccttcc caatgatcga
    49441 catattttaa tttcttttca taagcagact ggaaggaata gaaaaaatta ccataattat
    49501 atacgatatg catgactttt tcttgaaaga aagaaaaatc tgtttctcca acattagcta
    49561 atggagctaa gcctagattg gcttccgagt agcccatttt ctcgtataca ataaacagat
    49621 tggctaacag atagtcagct aattcctcag attctttagg taagacccgc aacaaatcat
    49681 atcccgcctg cttcttcgtc atcggctgag cggtgatgaa gccaacgact ttttgatctt
    49741 tacggacaat accgacaccg ctagataata catatgattt atcaaatcgt cccacagaga
    49801 agtatttttc tcgtgcacct tgaagccatt tttgtgaaac ttcatttaca gcttgatgta
    49861 attcttccgg cagttgatcg taccaagtaa aatggtatcc ttctttcatc aaacgttgat
    49921 attcaagttg ctgactcagc cgagacaagt cttctgttgc tagatcaaca atccctgctt
    49981 cacctacttt cgtgaagtga aaacccaatt catgaagcat cactacgtag ctttctgata
    50041 tcttataaaa cgcaagctga tagcctaaac aatctgcttg tttcataaac tgattcgttg
    50101 ctgcccgcat cattttagga ttgccaactg gatctcctaa aacaaagcat ttgtttcctt
    50161 tgatctgata accaaaaacg acttgatctt ggtcgtcttc ttggtaataa aaataactgt
    50221 aacctgacaa gcgaagctga tggcttgtag ctgtgcctcc gtatgtttcc aataaacggt
    50281 caaaacggtc tttttcgtac tcactgccaa gcttcgtagt atcttcagct aaatattgat
    50341 aaagcgccac taaagctagc aatgagatgc ccaaaccaat aagccctgag aaccacacat
    50401 catctgaagg aaacaaaata aaatgatttc ctgttattcg accgactaaa aagcgtccgc
    50461 tgtgatagcc tgcaattgcg taaacgataa acaaaaagcc gaacaagcta gcatcaaaga
    50521 ttatagctcc ccaagaataa acgaattttg ttcgataaaa ttcttttctg gataaccaca
    50581 cagctaacag aatcaagaca tataccaata tcaactgcca agataacgta cgtgtgattg
    50641 tatttaaaat accaaaagat agtaaagcaa tcgtaggcag aaatgccttt ttgactttta
    50701 ctgaaagtgc tctagcaagg ccgagtaata aaaagccaac caatagattc actgtctgat
    50761 ctaaaaaatt gaatgagaac ggcaataata cttgaaatag ccgactgatt gcagacagat
    50821 tagtgattgt tgatagaagt accatcatga tccccgcaaa atagacagca cttacgagca
    50881 cgatatgcgc aaccttttgg aaaaacagtc gcggaatgtt atctaaaaat cgattgattt
    50941 tgactcctgt atgtgtgatg aacaatagaa gtcccgtcat aaaaggtaga acataataaa
    51001 acaaccgata atataatagc caaactactg ctacatcttg ccgaacagac aattgactca
    51061 ttcctaagat catcaaaacg tcaaaagtcc ccattccacc tgggaccatc gtcagcattc
    51121 ctatcaatgt agcaatcatg aacataggat agatcgaaag catcgaaata tctattctca
    51181 tcaacttgcc gatgatcaaa aataccgcaa gggctcccgt ccattgtcca aaggatgcca
    51241 gtagcagacc aagcaagcct tttggataaa actcgaagaa caatgtcttt ctctttagat
    51301 aagcaaaaaa gaaaatgact ggtgctaaaa aactaccgcc gaaaagccag atccaatagc
    51361 ttcgaaaaat actttctgac tgaataaaaa agacatcaat aaatgtaaga aacgaccata
    51421 cagacaaacc agtcagcata aatagtgcga cttttgatac agtggctaaa accatttttc
    51481 tatttgtctg ctttccataa aaactagccc gcagacttgc tcctaccaca ccaccaaaac
    51541 cggcgagatt gttgatcgtg tttgttaccc acgcagcaag aagaaactct tttctttcca
    51601 tttttggttt tccttgtttt tctaagactc tgatcgtcac ccaatcatac aacaacatcg
    51661 gagtgacacc cagcagtcca gccagaatca tgagcaggat cgtcatgcgg ctttgttgtc
    51721 ccatcctttg ccaaatatct tgccaactca tgccattagc aatatttgcg acttgatttg
    51781 ccacaaagac taacacggag ccgaaaaaaa tcagttttaa aatcagacta tgttccttta
    51841 tccattgcag catcaagtat cgtttatttt tcacattttc ccattcctaa cttgtatact
    51901 tttgtgtcat accccaatgt tatcttattt attttaattt ttccacttta gacctgttct
    51961 ttgcttgcga tgatagaaaa agaaagatat cctaatgcta aaggagagga taacatgaaa
    52021 gtaggattta tcggcacagg gaatatggca caagctatta ttgatggttt attaaaaaaa
    52081 ggaacagtct cgaaagaaga ggtgttcgtt tccagtggac attttgaaaa tgcacaaaaa
    52141 tttgctgctg aaactggcgt ccatgcatgc aaaagtaatc aggaagttgc atcacaatca
    52201 gatatcgttc ttttagccgt gaaaccaaaa atcattgaaa aagtattgga agaactaaga
    52261 gaatatagcc agaaagtctt attcatttct attgcttcag gaaaatcatt aatagattta
    52321 gaaacagcgc ttgaagatcc tcaggcctcc atcgtacgtg tgatgccgaa tgtcaatgtc
    52381 agcgtaggag caggcgtttc tgcgatttgc aaaaatgaag ctgccacgga ggaacagctt
    52441 cagcaagcta cgatgatttt tgaagcaatc ggcagcgtct atcctttagc agaatcagat
    52501 ttcagtacat tcattggcct tgctggcagc tcacccgcat tcgtttacat gctgatcgat
    52561 gcgatgggac ggactggtgt actgaatggt ttgtccaaac agacagccac tgagattgct
    52621 gcaaaagcaa ttatgggttc ttgtcaaaaa ttcctttcaa gtgataaaaa tccatgggaa
    52681 cttgtagacc aagtttcttc tcctggcggt acaactgttg caggtgtagt tgccttagaa
    52741 gaagcaggat tcatcccagc aatcatcaaa ggaatcaata cgacgatcga gaaagatctt
    52801 gagatgttga ataaaaaata atgatagcaa tgttttttcg tgccttacaa aaaaagaaga
    52861 gctgtggctt ttgccgcggc tcttcttttg tattttttat tatttagcat aatcagttgc
    52921 acgagtttcc cgaataacga ttactttgat gtgtccagga taatccagtt cttcttcgat
    52981 tcgtttacga atatcacgta ctaaacgaac agcgtctaaa tccgagattt cttctggctt
    53041 gaccattacc cgaacttcgc gtccagcttg aactgcgtaa cttgattcta caccggcgaa
    53101 gctgttcgaa atattttcca gacttcttaa acgacggatg tagttttcta atgattcact
    53161 tcgcgcacca ggtcttgctg ctgagagcgc atctgctgca gctacaagga cagagataac
    53221 agaagtagct tccacatctc catgatgaga agcgatcgcg ttgattacta ctgggttttc
    53281 tttgtacttg gcagctaatt ccgcaccgat ctcaacgtga gatccttcaa tctcatggtc
    53341 aagtgctttc ccaatatcat ggagcaaccc ggcacgtttt gctaattgga tatcttcgcc
    53401 aagctctgct gctaagatac cagctaattt tgcaacctcg accgaatgct tcaagacgtt
    53461 ttgtccataa ctagttctga atcgcagacg tccgatgatc ttgattagat ctggatgaag
    53521 tgtatgagca ccgacttcaa acgctgcctc ttccccgtat tcgcgaatac gttcatccat
    53581 ttctttacga gatttctcga ccatttcttc gatacgcgct ggatggattc gtccatcttg
    53641 tatcagtttt tccaaggtca tccgtgcgat ttctcttcgg attggatcga atccggaaag
    53701 aacaactgct tccggtgtat catcgatgat taaatcgata ccagtcagcg tctcaagcgt
    53761 tcgaatatta cgaccttctc gtccaatgat acgacctttc atttcatcat tcggaagggt
    53821 aacgactgaa acagtcgttt ctgaaacttg atctgctgcg cagcgttgaa tcgctaatga
    53881 taataagttt ttcgctttgc gatctgcttc ttctttggca cgctgttcag attcttttac
    53941 cattagagtt aactcatgat tcagctcttc ttcagtcgat ttcatgatga catctcgtgc
    54001 gtcctctttt gataaaccag caattttttc tagttctttt tgttgatctt caatcatttt
    54061 ttcaacgtct ttttctcgct cttcaattaa ttgctgtttt gaactaagtg cttcttcttt
    54121 actttcaagt gagttctcgc gtttttctaa cgagtcatct ttacgattca gcgtttgttc
    54181 gcgttgaatc aaacgattct cttgagattt taattctaac ttactttctc tcagctcact
    54241 ttcaacttca gaacggtatt tctgattttc ttccttagct tctaacaatg cttctttttt
    54301 tagagtttct gcttcttttt ttgcactatc gatgattcca gcagctgatg attgtgcacc
    54361 agctatttct ttctcgtgac gtgactttgc gaccataaac cctaaaccaa gaccgacaat
    54421 taaaccgatg atagcgagga gaatgttgaa taccataaaa tccacctcca tactatcttt
    54481 ttcattttta tcatctatac agcttctatg ataaattatt ctaaactcgt caatatgcat
    54541 cttcgcatat atgtgtacaa aaaacatgca caactttatt ctaatgtttg taccccgtag
    54601 tgtcaacttt aaataaacaa agaaacggct ttctacttat tcattaaacg atggttttta
    54661 aaataaatga cactagtttc atataggtaa acctttaaat tgagaaactt ttctattaaa
    54721 ttattctttg tagatgaaca gatattttta ttttatgtac ccactcgtat aattaaataa
    54781 ctcacgattt ctttattcaa taaaaaaacg gcaggtcaag gagagtacct actcttcaga
    54841 ccagccgttt ttgtttatca taatttttat tcttctacta aaggcaattc ttcttgtcct
    54901 ttttcatctt ctgggacagc gacttcttca ccaataccat aagctgctct aactagagca
    54961 gaaacttctg ccatcatttc aggatgatta gccatataga ttttagcgtt ttcccgtcct
    55021 tgaccaatcc gatcttcttt ataagaatac caagcaccgc ttttatcaac aatgtctttt
    55081 tctactgcca tgtctagaag ttctccttct tgtgagatcc ctaaaccata catgacatct
    55141 acttcagcga ttttgaaagg aggtgctact ttgtttttta cgactttgat ctttgtacgg
    55201 ttaccaacaa tatctgtacc ttgtttcaat tgttctgcac gacgtacttc caaacggatc
    55261 gttgcgtaga atttcaatgc gcgtcctcca ggagtgattt ctggattccc aaacatcacg
    55321 cctacttttt cacggatttg gttgatgaaa atagcgattg tttttgtttt gttgatcgaa
    55381 ccagagagtt tacgcaatgc ttgagacatc aaacgtgctt gtaacccgac atgtgagtca
    55441 cccatttcgc cgtcgatttc tgctcgtgga actaaagcag caactgaatc gactactacg
    55501 atatctacag ccccacttga tactaaagca tcagcgatct ctagaccttg ttctcctgtg
    55561 tcaggctgtg aaagaagtag ttcatcgata ttcacaccta atttttgtgc atattgcgga
    55621 tctaacgcat gctcagcatc aatgaaagcg gccgttccgc catttttttg tacttctgca
    55681 atagcgtgta gtgcaactgt tgttttacct gaactttcag gtccatatac ttcgatgata
    55741 cgtccacggg ggtaaccgcc gacacctaaa gcgacatcta acgcaagaga gccacttgga
    55801 attgtagaga tttgttggtc gattttttcg cccaatttca taatagagcc tttaccatag
    55861 ctcttttcaa tttttttcag tgcagcatct aaggctgctt tacgatcatc tgccaatacg
    55921 ataatcctcc ttatatttac aaatcagagc aacatttcat tatccttatc atatcttttt
    55981 tagtgaaaaa aagcaagaaa aaaccgaaca aatattcgca tttatcattt tcttaaaagc
    56041 tgttttctca aaagatcaaa tccttgcata accgcattat atctgatagc agcacgatct
    56101 ctggcaaagt gatatttttc cgaaaccacg ccgtctttag atgaaatagc gatccatact
    56161 gttcctgcag attgaccttc taacgtgtcc ggtccagcca ctccggtgaa agctacactg
    56221 taatctgttc ttgctatctc acgtgcttga atggccatct gctccacaca ttctttgctg
    56281 actgttccat attctgctaa tagctccgga tcaatctcca gaaaaccagc ttttgtttgg
    56341 agcgagtagg taacgaatcc gcctggaaat acttcagata caccagcaat gtcgcctaat
    56401 gcggcttgga aggcgccggc tgtcaaactt tccgctgctg ttacagtctg cttgttttct
    56461 ttcagtagtt ccactacgac ttttgctagc gaattatcat cgccgtatcc ataaaaatat
    56521 tcccctactc gttcttggat tttttcttct aatgctaaaa gagcttgatt ccctgcctga
    56581 acatcatttg ttttcactgt cagtcgtaat gtcacttcat ttggtttggc atatggtgcg
    56641 atcgttggat tgatctgtgt ctcaatcaga tcttttagct cagtgaccag tcgcgattca
    56701 ccaatcccat aaaagcgcaa aactttagaa attagttttt cttcagaagg aaaacgttcc
    56761 tctagtaaag gacgtacttg ttccacaaac attggtttca actcattagg cggaccggga
    56821 agaaggatat acgcatgtgt atctgtttga taaaaagtcc ctaaagcaag ccctgtacga
    56881 tttggtagag gaaccccgcc ttcgataatc tgggactgtt gcagattgtt tttggtcatt
    56941 tttcgatggg ttgtttcaaa atatgccaat aattttttgt atccttccgt attttgtatg
    57001 agagacttac cgaggtgagc cgcagttgct tcttttgtca ggtcatcttc tgtcggtcct
    57061 aatccgccac ataagagtat gagttcactt cttgtttcag ctagagcaat caactcttct
    57121 aaacgctgct gattatctcc tacaactgtt tgataataaa cttcgatacc tagatcagct
    57181 agctgttctg acaagaaagt ggcattcgta ttcacgactt gtcctagtaa aagttctgtt
    57241 ccgacagcta ttatttctgc tttcattact tcccgctccc ttatatagta tacgctgtat
    57301 ttttatattt ttttatttaa aaagagggca aggagctgtg acaaaaatct tgtcacagct
    57361 ccttgttcca aataaacagt gttctcctgc caaaacctcg gtcataagtc acaatataca
    57421 taaaacatga aaagctgttt tttgaaatta tcccttgtga ctcagagtta gacggcaggc
    57481 tcacaacctc tccactaaac gatacttaca ttgatccttt gaacacaccg ctgtttttga
    57541 tgaagtaatc cacacctgaa tagatagtaa agatcagaca agcataaagc atgatttgat
    57601 ctactggaat accgatcagg ctgaatggga tattattgat aaacagcaag ataatagcaa
    57661 ccatttgtgt agccgtcttg acttttcccg gccaagctgc agccatgact tctccatgtt
    57721 cgacaagtag taaacgcaag cctgtgacgg ctaattcacg acagacaatg atcgctacta
    57781 cccaaccagg ggcctttcct tggtcaacca acatgataaa agcagtcata actagcattt
    57841 tatctgccaa cgggtccgca aacttgccaa aattagtgac aaggcctcgc gcacgggcaa
    57901 tttttccatc taaccagtct gtgatactag ctaaagcaaa aataactgca cctactagct
    57961 gtgtgacagc cagtgtggtg tctcctacgt taaattgtcc ccaatctaaa ggtaaaccta
    58021 caaccaaaat gaaaatcggg atcatacaaa ttctaagtac agttaattta tttggtaaat
    58081 tcacactatc gactcctttc ttccctattc tacgtgacta tcagcgaagt ttccaccgat
    58141 attatttact tttccaaatg aatttattca aaggagtatt tttattgaaa ttgatctttt
    58201 tgaattaacg aagaaggacc gcgacacaga tttgtcacga tccttctttt ttatacctac
    58261 aagtaaacag atgcgtaaac taatttatac gatcattctg cgtattgaag agtaaggttc
    58321 acattttttg gactctgatt gttttcccct ggattaactg gtacggattc tccattcgct
    58381 ttgatcgttg cattgcttgc tgcaccaatc gtgatggtcg catttgttgc tgtttccggt
    58441 aacacggtac tctgtgtttc atttgctgcc agtgtccctt gataaacata ggcattgtct
    58501 actagcacac ctaaccatac acgattgaca gcagtaaatt caagggttac aggcttggta
    58561 gcgtctgtga tatttactgc gatggcactg ccagaatctt ctcctgtagc tattgccatt
    58621 ttcttttctt cacttgattt tgttgttgat tctactgtag aagactcttt ggtctgcgtg
    58681 ctttgctctg aggaagagga ctcctcgact gatccttcta ctttaagcga agaagctgtg
    58741 tctccgatta ttggatcagc ttgacgatct tgccaagtca tatagcccac gacaacgaca
    58801 atcgccaaag caaccaatcc taataaaatg acaggaagac ttgtccaaaa gcgacttgga
    58861 tgcgattctt cctgatgcat tgcttttcta gaagtactta ctgtttcagg ctgcggacgt
    58921 tcagggaccg atcctgtcaa tttgcttttc ccatcaaaaa catcgactaa atggtctcca
    58981 tcttctccta cagcttcagc gtattgccga ataaatgccc gcacgtaaaa tttaccaggc
    59041 aattggtcaa aatcatcatt gtcgattgct tccaaataac gtttttgaat ttttgtaatc
    59101 tgctgtaatt catctaaaga tattttttta ttcaggcgtg cttgccgtaa tttttctccg
    59161 atggtttcaa aagccactcg ttcatcttct ccagtctgtt tgattctccg aagcatctgc
    59221 tgttgcaacg aaagacgtta tgtttgggtg ctgtgacaaa agtcatgtca cagctccttt
    59281 tttccgaata aacattgttc tcctgccaac tcctcggtca caagtcacaa tatccaataa
    59341 atataagaag ctgttttttg aacttgttcc ttgtgactcg gagttaaacg gcaggctcac
    59401 gacctctttc ttaaacggtg ttcaatcagc tgttcctcgg tattagctca aaaaccggca
    59461 aaatataagg agatattttt ccgtatgttt ctctaatact caggtcaaaa cgctgatttc
    59521 acaacctctt ccttaaacgg tgttcaaaag cttgcatctg cggtaataag tccaaccaag
    59581 ttaaaaacat taagaagctg tttttacttg gctgttctta ttacttgatg cagggcagct
    59641 ttttcacaac ctcttggtac acggtgttcc agaagtagct tcttcggcaa taagccgaaa
    59701 tatcacaaaa atttgagaag caattttcgt aaattccttc ttatttcttg aagctgacca
    59761 cttctgtcac aacctctttg attacttcgt ttgattttat tttaatataa gttgattaaa
    59821 agagagcatg atttgtttta ttttctcact aatttttcca acacaactaa gaataccata
    59881 aaagctgatt ttggaggagg ttcctactac aagtctaaaa cgaaatttaa gacttttcct
    59941 cataaaatac tggtttattt tggtctcatg taaaactcgc tgaaggcctc ttcacttata
    60001 aatgcttccc cagctgccag tacgtctttc atttggatcg aatcgatgat ttcaggaaga
    60061 tcaaataaag ttcgatcgcc aaataaactt tgcgtaaatt gattggcaat atattcgatc
    60121 gaatttaatg attggaaata ttgtccaagc attttcttct tcagcaaatc gagatttgtt
    60181 tctgacactt ccggatcatc agcgaatcca agaatgattt ctttgacttt ttctgctgct
    60241 ttctctggtt cgtccgtatc tccgctgaag tccgcaaagt gaaattcacg atccaagctg
    60301 aattcaaaac caaagctgtc atcgataatt ccttgattgt acatcgctag atagtttcga
    60361 gaagtattgc ctaaaatcat ttggaacaac agattgattg ctgttttata gcgtaacagt
    60421 tctgttcctt cttgcggcaa tgtgtctaat cctttaatac caagtacaaa tttatctcgt
    60481 gtaatggctg cttccaatgc actttgtttg atgatttctt ttgtgttttc agggaaataa
    60541 cgtacaattt cttgctttgg agggaaattt tttgcttctt ggttttcccg aataagtttc
    60601 atcaatttct caggctccat cttaccaact acaaataaaa ccatattgct tggctgataa
    60661 aacgtccgat agcacgtata caagtcttga gctgtgattt tgtcgatact ttcaaccgtg
    60721 cccgcaatat cgatatgcaa tgggtgtgta ggatacaagt tatttagtat gccaaaaaac
    60781 atccgccagt tagggtcgtc ttcatacatt tggatttcct gaccgatgat tcctttttcc
    60841 ttgtttacag tttcttctgt gaagtaaggt gcttgtacaa aatcgatcag agtcgtcaga
    60901 ttcttttcta cctgatccgt cgtagaaaac agatagctgg ttttcgtaaa acttgtgaaa
    60961 gcattggcgg aagctccttg tttcccaaat aattgaaaga catcaccatc ttctttttca
    61021 aacaacttat gttctaaaaa atgggcaatt ccatctggaa ctttcacctt ttctttttca
    61081 ccatagggaa taaattcgtt atcgatcgaa ccataattcg tactgaacaa accatatgtc
    61141 ttgtgatagt catttttagg caacaaatac acggtcaaac cgtttggcag tacttcatga
    61201 taaagtgttt cattgatttg ctcatattct gttttattca tttgcatttc ctcctgctaa
    61261 aaagaagatt gcctgcaatt cgatttgctc agctacttgt tgtatctctt tgatcgttac
    61321 gctctccata cgtttcaacc attcttcatc agataatttt gtttccggca gccagctgtc
    61381 caaaaaagaa gcttcgattg ccgcttgcgg agagtctaga gaaagcagat attggtttct
    61441 cagcattgct tttgtctgag acagttccaa ctctgttatt tcaccatttc gcaaactttc
    61501 taattgctcg tgaatcagat gaagtacttg atttcggttc ttttcatcga taccggtttg
    61561 gacactcatg aatcctcgga acgtatccac gctactagag gcataataag ctagactttc
    61621 tttctctctg acattcatga aaagtttaga gtgaggaaaa ccgccaaata aaccgttaaa
    61681 gaccattagc gcaaaacgtt ctggttcatc gtaatacaca tttgtctgat aagctaggtt
    61741 cagctttgct tgaacgattg gctcacgtac ttgtcgttcc ttcacgatat tcacttgagg
    61801 ttgggtataa aacagatctg gataaactgt ttcccgcgac tcaaatggta attgcccaac
    61861 tgcttcagct gctttgtccg gatcaacatc gccgacaaca aaaatgtcga tttgatcatt
    61921 tgccatcatt tcttgatagt aggacgctag cgaactagca gtgatcttgt ccaaatcttc
    61981 gactacgccc aaccctggga tcttttgttc cgggctgttt tcaaaataaa gttcttgcaa
    62041 ggcataggaa gcaaaagttt gtttatcttc cttcaaactt tccaaatata gacgcagatt
    62101 gttcttttcc aagtcaaacg tcaactgatc aaactgatga tttttaatat ttggataaaa
    62161 taaaacctct tttaaaaaat ctaccgcttg agaaaaaagg tttggatcat tgacatattc
    62221 accattcaca aaactgatcc ctgcgtttag ccaatgtaaa tttccttttt ttctaactga
    62281 taatccaaag cttgcgccat aaagctctgc aagttgcgca cttaactttg tctgatccga
    62341 ataattcaaa ctatttgttt ccaacatgct gctgagaagt gaacgtttcg tgatgacttc
    62401 ttgttgcaga cgcgcagtaa aacggataaa aatccggatc gttttgtatt gttttgttgg
    62461 taagacatga agattcacgc cttctgccaa ttgatacacc atatttatat gctccttttc
    62521 acagttgatt gcccgcttac tcttttaatg ggtcaacctg ccttttttac ttattcctct
    62581 gcttgaaatt tataagttca ggcaattcgc cgttttttct aaatgatgtt acactttata
    62641 gtatagcata gatgtttttt atcaaaagaa acagacggct aaaagcgtat attgtttttt
    62701 tattttttat ttttcgatat actaaagaca aataaaagaa gggagggtca ttcatgatca
    62761 aagaattcaa agagttcatc atgcgtggag atgtcttgga tcttgctgtc ggcgttgtta
    62821 ttggtagtgc gtttacaagc atcgtaaacc aagtggtgca aggcttgatc acaccactaa
    62881 taggttggtt tgtcgcaatg atcacaggta cttctgattt agaaggagca ctttcaattc
    62941 ttgactggtc gccgacaaaa ggcgtcacat ttgcgtttgg taacgttatt agtgctatca
    63001 ttacatttct gatcactggt tttgtcttat tcttagttgt caaagcggca aaccgtgcga
    63061 agaatttacg ctccaaagaa gagcaagtgg aagtggaaga aactcctact gcagaagatt
    63121 atcttagtga catacgcgat cttcttgcca aacaagtggc agaagacaat acacatccag
    63181 tagagcaaac aaatctttcc gatacttcta aataataaag aatctaaaaa aaaagaaagc
    63241 cgattttcag tgtcggcttt ctttttttag attcttaact tgctacttgt tcctttttca
    63301 tgtcgatatg tggaatatca tcttccaaat acacatctga gattgccttg aatccaaacg
    63361 actcataaaa agcttgtagg tattcttgtg cgccgatcac gattggtttt tctggaaaat
    63421 ttttctggat catgttaatc gtttcttgaa caatctttct tcctaaacca tctttacggt
    63481 tgtcctgatg aacaaggaca cgtccaaaat ggatactctc cgcttcttca tatactcgcg
    63541 tgtaggcaag gatcttccca tcagtatctt gaaaaaacgt atgccaagct tctccatcaa
    63601 tgtcatcaac ttcttgataa ggacactgtt gttccacaac aaaaactgcg attcgttctt
    63661 tgacgatcgc gtaaaactct gctgttgaca attcttcaaa tttctttacc tgatattcca
    63721 ctaagcaaac gccccctgct tttttattct tagcataccg attcgcacac aaaaaagcaa
    63781 acgaacctta cgaatctcag gctcgtttgc tttttaaact gcttcagtta aaaattcgat
    63841 ctcaactgtc cgagtaagta catctgaata gctgtaagat actcgttcaa aagaattttc
    63901 ttcaggatcc aaatcaacaa cgaatactga aggataggtt tctgttaaaa cgccgcggcg
    63961 ttctgtttga cgctttcttc ctgtctgagc aaccaacatg attgggctgc cgatacgacc
    64021 ttctaaatct ttcttgattg aagctaaagt tgttggcata cagttcacct ctactatcaa
    64081 ctataccaca tatttttaaa attttcaagt ttaccataaa aaaaaaagaa atgcaataaa
    64141 aataaataag aagctcatta agatttttct aaattattcc tcattataaa atttcaagtg
    64201 tttttttatt ttgagtttta ctctcccgta agcgctcatt acttgcttgt gatccattgc
    64261 taaacattca gccatgtcta caaaatcaac gccttgcata taataatgaa aaattttagc
    64321 ttctattttt gaaaactgga tttcgcaatt ttcgattgct tcttttaata aaagatgctg
    64381 aattgctaaa ggttcttgca atgtgatctc tgctaaaaag tctggtcctt ctgtctctat
    64441 tttttggtcg attgagactt catctttttg atcacgacgc ttcagcgcgt tctgcttcct
    64501 caacagagaa tagatttgat tgcttataac gcttttataa taacttccaa agctaacacc
    64561 tttgcttttt tcgtaagttc tcaatgcctg atataaagcg atccttgctt cttgttccca
    64621 atcctcagaa acataatttc ttaaataata ggtgtttttc aaacgtagta aaagtggaaa
    64681 atatttttga tacagggcag caaatgcttc ttcgtcacca ttacgtgcag cgcgcaataa
    64741 ctcctcagac ataaaataat tcctccataa attcgtttat ggaggaatct tatcatcaat
    64801 gattcatcga actgattaga acgatatgtt atttattttt tctcattaaa tcgtccaatt
    64861 tttgggacaa tttttttaac tgctcttcat tccatggaga attacgtcgg aaatcaataa
    64921 aatgcatatc tttattttct tgggctattg ttttttccgt tttttttatc gtcttatata
    64981 attcataagc agaagttcgt aatgctcctt gggaaaagat catccactgt tctgctaaat
    65041 cgcttgtcgc cactgtcact tgtgtcaaac ggtcattcag ctgaccagcg attctctcga
    65101 tataactgtc agccgtttcg tcttctttcg taaaaacaac atccaactgg tatttcttat
    65161 aattttgttg gatgcccgga actagttggg catcgaagac aacgatgact tccattcctt
    65221 catatttcgc ataatttgat aaacgatgta aaagagcttc tcttgcatcc gccattttgt
    65281 cctgcttttt aagctggaca agttccggcc acgaaccaat catgttgtag ccgtctacaa
    65341 tcaacagctg ttttttcatg gtctactccc cctgacgttg ggaaaaagcc tgatacataa
    65401 gcaaaccagc agcaacactg gcatttaagc tctggacgtg cccggtcatc gggatcgtca
    65461 gcaattcatc catttctttt ttcaatcctg gactcattcc tttgccttca ttaccaatga
    65521 ctaaagcaat tgatcctttg gcattccatt tttggaagtt tgtgccggtc atatctgtgc
    65581 caaataccca aaatcctgtt tctttgagct gttgcacagc ttgtaccaaa ttcgtcacgc
    65641 gtgcaacagg gacatgttcg actgccccag tggaagtctt tgttacgata ggtgtgattc
    65701 caactgctcg gtgttttggt atgatcacac catcgacgcc acttgcgtct gctgtccgta
    65761 aaatcgaccc aaaattgtgt ggatcttcga tgctatccaa aatcaaaaag aaggggtctt
    65821 cttttttctc ttttgttcgt tcaatcagtt ggtcaaggga taaatattca taaggtgtaa
    65881 tcgcaagaac gactccttga tgcacgccgt gatcgctcaa agtttctaat ttttgtttag
    65941 gaacccattt gactggcaca gcttgttcac gtgcgatttc tttcagttct tctacttttt
    66001 ctcctcgtga atcttcttga agaaagagtt tattccctcg cccttgttgt agcgcttcta
    66061 ctgcggcatg gtgcccaaag acgaaattat cttctaatgc ttcttcattt tgatcagaaa
    66121 ctgactgatt tcgtccagtt tgctttttat ttgaatatct attttgtttt ttgtcttcaa
    66181 atccacgttt cgattgatgt ttgtttgttc tttctttacc catttttttc accaactttc
    66241 tgaatacacc agcggatcaa ttcttccatc cgctcggttt gttttgtcaa atgcagatag
    66301 cccatgagcg cttcgaaccc tgttgcgacc cgatacgtcg taacatcagc attttttgcg
    66361 ctcgtatggc ttttagcatt tcttcctctg cgataataaa gttcttcttc ttctgtcaaa
    66421 agatgttctt ccagcatttg ctgaatcaaa aaggcttgtg cattcgcaga aacataattc
    66481 gttgccgcac gatgcagctt attgggtttt gtttctcctt gatcaatcag gtggtcacga
    66541 acatagattt cgtaaatcgc atctccaaca taagccaagg ctaaaccgtt caattgtgtg
    66601 taatctctca tgcttctctt ctccatcggg ttccttgcgg tgtatcttct aataagatcc
    66661 ctttttcttt caacaaatca cgaatttcgt cgcttcggac aaaatcacga ttgcttcttg
    66721 cttgatttcg ttcttcgatc aattgttcaa tctcttcatc taataactct tctgctgtaa
    66781 ataaaatacc gaagatcgaa agccattgcg agaataaagc ttctccttgt gccaagacag
    66841 cggcagaaac tttcggctgg tctagataag tgttcagcca cttagcaagc tcatagacca
    66901 ctgtgatccc atttgctgca ttaaaatcat cgtccatttc ttcgatgaat cgctgttcta
    66961 aattttctaa caaagcgatt ttatccgcat catctgccaa attctctgag gcagtttctt
    67021 ggcggaattt caaattgtca aacgtattct tcagtcgttg taaattggtg gctgcttctt
    67081 tcatcgtcgt ttcactgtag cggatcggac gacggtattg tgtggtcgcc atgaagaaac
    67141 gcaagacttg tggatcaacg tttttgatca tttcatgtac agtgatgaaa ttccctagag
    67201 atttactcat tttttcatcg tcttcgccaa tcgttacata gccattgtgc atccagtaat
    67261 tagcgaattt tttccctgtt ttcgcttcac tttgtgcgat ttcattttca tgatgaggaa
    67321 attctaaatc ttgcccgcca ccatggatat cgatcgtttc tcctaaatgt tttgttgcca
    67381 taacggaaca ttcaatatgc caaccgggac gtcctttgcc ccatggtgaa tcccaagaaa
    67441 tctcgccagg ttttgcttgt ttccataaag cgaaatctaa tgggtcttct ttttgctgct
    67501 gttcttcccc tgtacgctga cttgctccga cttccagttc atcgatggat tggtcactta
    67561 atttcccata attttcaaat ttacgggtac ggtaatacac atcacctgaa gattcataag
    67621 catagccttt ttcaaccaac acttgaataa atgctaaaat atctggcata tgatccatga
    67681 ctcttggatg cagtgtagca ggttcgacat tcaacgcttt cgtatcttct tcaaatgctc
    67741 ggatgaaacg atcagcaact tctggtgctg taatacctaa ttcgtttgct gtacggatga
    67801 ttttatcgtc tacatccgtg aaattcgata cgaaatcgac tttatagcct cgatattcaa
    67861 aatagcgtcg aattgtatcg aaagcaatgg tgctacgagc atttccgata tggatataat
    67921 tatacacagt cggtccgcag acatacatac cgacatgtcc cttttgtata gggatgaatt
    67981 cttctttttt tctggtcaat gtattgtaga tttttatcat tctgatctcc tcactttttc
    68041 tcctttgatt cgaacgactt ttgctggtac tcctaccgct gtagcgtcgt ctggaacatc
    68101 tttcaacaca acggcacctg caccgatttt ggcgtttttt ccgatcgtta ccggtcctaa
    68161 tatctgcgca ttcgcagaga gcatggctcc ttgtttaaca gttgggtgtc ttttacccgt
    68221 ttcttttcct gtgccgccta atgttacgcc atggaataaa atcacatcgt cctcgatttc
    68281 tgcggtttcg ccaatgacga ttcccattcc atggtcgatg aacacgccag ttccaatcgt
    68341 tgctcctgga tggatctcaa caccagttag gaaacgccag aattgagcat tgatcttcgc
    68401 caataaaaag agccggtgct gatataaaaa atgagagaaa cgatgccaaa acaaggcatg
    68461 aaaaccaggg tatgtcaaaa cgatctcagc tgtcgaacgt gcagccggat cgtttctctt
    68521 tgctgcatca atcgctcggg taaaccaact catgactttc cactcctttc gtaagaattt
    68581 tatgtaggaa atcatcttct tataaagaaa agaagcgcct tttaaataag tttccttatc
    68641 aaaagacgct ttccgcgtgg ttccactttt cttcataatc aatgattatc tctgacagct
    68701 aacgcctgtt atacgttctt ggctactttg tttcctataa aacagttttt cctgttttct
    68761 gtgtttcgcc aagaccactc ccagaggcat ttcggcaacc attcattgat accttgcacc
    68821 acccggtatc tctcttgtaa tgatcagctg cttacttttt tctgttcatc gtgtttagtt
    68881 cagtacttga tttaaatgtg ctaatgattt ttcacgacca agcaattcga tcgtttcagg
    68941 caattctgga ccatgcattt gtcctgatac agctacacgg attggcatga aaagattttt
    69001 tccttttacg ccagtttctt tttgaacagc tttgatggca gctttgacag ttggagcatc
    69061 tactacttcc atttgttcta gctgtgcttt gaatgcgtgg agaacagtag gaactgtttc
    69121 accagcaagt acttcttttg ctgcttcatt caatacagga tgttcattga aaaatagcgt
    69181 agacagatca acgatttctg cagcatagct catttgaggt tgatacaggc taacgatctt
    69241 cttcacccat tcgattgtct tttcatctgg gttttctgct acacgtccat cagcgattag
    69301 ataaggcaga cacatgtcag taaatgcatc tagatccatt gctttgatgt aatggttgtt
    69361 tacccattgc agttttttcg catcgaaagc tgcgggtgat ttacttaggc gttttggatc
    69421 gaacattttg atcaattctt ggtgttcgaa gatttcgtct tcgcccacag gtgaccagcc
    69481 caataatgca ataaagttga acattgcatc tggtagatag cctaattcgc gatattgttc
    69541 gataaattgc aagattgatt cgtcacgttt gcttagtttt ttacctgttt cactgttgat
    69601 gatcaatgtc atatgtccga aggttggtgg tgtccagcca aatgcttcat agatcatcag
    69661 ttgttttggt gtattggcaa tatggtcatc tccacgtaaa acgtgagtga ttttcatcaa
    69721 atgatcatcg accgcaacag caaaattata agttggcata ccatcgcgtt tttggatgac
    69781 aaagtcgcca ccgatattat cggattcgaa cgaaatagcg cctttcacca tatctgtaaa
    69841 tgcgtattct gtattgcgag gcacacggaa acgtacaaca gattctagac cttgtgcttc
    69901 tttttctgct tgttcttcag gtgtcaagtt cgcacatgtt cctgcgtaat gaggcatttc
    69961 tcctcttgcg cgttgtgctt cccgttctgc ttctaattct tcttctgtgc aatagcattt
    70021 gtatgctcga ttgctagcaa gcagctgatc aatcagcggt tgatagattt ctttacgttc
    70081 agattgacgg taagggccgt attctccagg attttcagga gattcatccc aattcattcc
    70141 caaccaagca aggttttcca actggctttt ttcgccatct tcgatattac gtttttggtc
    70201 agtatcctca atacgaataa taaagtctcc atcattgtgg cgggcaaaca aataattgaa
    70261 taatgcagtt cttgcatttc caatatgcag gtgcccagtt ggacttggtg catagcgtac
    70321 gcgtactttc gtcattttct tcctcttctt tcaaactaga ctttgactaa cagcaaaaat
    70381 tccgtcagtt acggcttctc aaatatcatt tatttaaagc cactcagcac aaattttacc
    70441 ttttctaagg taaatagtaa agccaactgg ttgaatttcc ttaccagttg gctgaatttc
    70501 ttttttattt tgattgttta gtctttttct ctgtctcttt tttattgtcg attcctcgtc
    70561 ctgcatgtgc aggtttagca aagatcatgc gtccagcagc tgtttgaagg gcacttgtta
    70621 caacgacttc gatacgttca ttcatgtaat gctgtccatc ttctacgaca accatcgtgc
    70681 cgtcatcaag gtaggctact ccttgttggc gttctgtccc ggcttttact actagaacat
    70741 tcatcgtttc accagggatg acgactggtt ttacggcatt tgccaacgca ttgatattaa
    70801 gaacaggtac attttggaat tcagagactt tattcaagtt atagtcattg gttacaatga
    70861 caccatctaa aagcttagcc aatttgatca atttgctgtc tacttcactg atatcttcaa
    70921 agtctccatc gtacatttcg acagatatac cctcttcttt ttgcaaggca tttaggatat
    70981 ctaatccgcg acgaccacgc acacgtttca aactatcccc tgagtccgcg atgtattgta
    71041 gttcgtaaag aacaaaatta gggatcatca aggttccttc taagaatcca gtctttgtaa
    71101 tatcataaat ccgaccgtca atgatcacgc ttgtatccaa gattttatat ttatggaaat
    71161 tttctcctga ttttcgttct aacagttgac tctcaacttg actagctgtt tcttgctcga
    71221 tttttttgct tcgtgaacca aaaactttct tccattcttc gattcgtgtc gttcccattc
    71281 tcaatcccag atagcctaag atgatcatca gtaaaatcgg caatacacta ttgacgaatg
    71341 gaatttccat attgtaaaga ggggtcgaga tcacaacacc taaagtcaat ccgatgatcg
    71401 caccgatcgc accgaagaga agataagtca agctgacctc acttagtttt tgttctactt
    71461 gtttgataat agctgagata tgtttggcca acagcaaaga taataaaaag aaaataagtg
    71521 cgcctaacag gctgttggta aatgtattat tcagccatcc gtttgactgt tgaccgattg
    71581 cctgccaagc tgagggtaaa aatgaaatac ccaagcttgc gccgacaatg atcatcagca
    71641 aagtaatgac acgtttttgc atcgtttttc ctccgtttcg acaatcattt attagttagc
    71701 ggaacacacg cttcaacgtt tcagccaaag ttgctactcc aactatctcg attccttttg
    71761 gcgcttccca gttacctaag ttgtttttag gtaggtatac ttttgtgaag ccgagttttt
    71821 ggacttcttt gactcgttgt tcaatatgac tgacacgtct gatttctcca gtcagtccaa
    71881 tttcaccgat gaaacattcc gttggctgtg ttcctttttc tttatagctt gaagcaatac
    71941 tgacagcgat agctaaatca atcgctggtt catttaattt taccccacct gcagctttta
    72001 agtaagcatc ttgattctga agaagcaacc cagctctttt ttccagcact gccatgatca
    72061 gagatacccg attgaaatca agacctgttg ttgttcgttt ggcatttccg aacatagtag
    72121 gtgtgactaa tgcttggatc tctaccaaaa ttggacgtgt cccttccatt gccacaacga
    72181 tagcagagcc agttgcaccg tctaggcgtt cttctaagaa aatctgtgaa ggattagcga
    72241 cttcttccaa tccatgctca tgcatttcaa aaatcccaat ctcattcgtt gagccaaatc
    72301 ggttttttac tgctcgcaaa atccgaaaac tatgatgttg ttcaccttca aaatataaca
    72361 ccgtatccac catatgttct aacattcttg ggccggcgat cgagccttct ttggtaacat
    72421 gaccgacgat aaaaatggct atcccatttg ttttagcggt tttgagtagt tctgcagtgg
    72481 tttctcttac ttggctgaca cttcctgcca cacttgtgat gtctggctgc gtcatcgttt
    72541 ggatggaatc aatgataaca taatcagggg agatatgttc gatggcacga ctgatttcgt
    72601 tcatatctgt ttcagcgtat agataaaatt ctgtgttgat acttccgagg cgttccgcgc
    72661 gtaattttat ctgctctgcg ctttcttcgc cggatacata taagactttt ccgcctatgg
    72721 ctgctaattg ctgagaaact tgtaacagca aagtggattt cccgatacct ggatcgccac
    72781 caatcaagac catagaccct ggcaccacac ctccgcctaa cacacggttc agttcttcta
    72841 attttgtttt gatccgaggt tcttttttag ggacgacgtc tgcgattttt gttggttttg
    72901 ctttttctcc tgtcaaactt gtccgagttc tgcggtccgt tgtatcttgt tcgatttctt
    72961 cgaccatcgt attccatttt ccgcaatttg ggcagcgtcc caagaactta ggcgacacat
    73021 acccgcaatt ttggcaaaca aattgtactt ttgatttttt tgccattgta ccgcctcctt
    73081 ttatcctaat catgagacat taaatcaagc ttaagtatct ttaacgtttt atttactgga
    73141 agaaccaaat ccgccttgac gttgagcagt cgtttgatct tgatctgctt ttaaaaacgg
    73201 caaaaagatc ccttggccga tacgctcgcc ttttttgatc actacatcct tgaaaccaaa
    73261 gttggtgaat tggaacatga tatggccttc atttgcctca ttattgtaat aatcactgtc
    73321 aatcacacct actccgtttg tcaacactaa aaaccgcttc aacggattgc ttgaacggtt
    73381 agcaagctgc aaaaattcat cttctcccat gtaagctttg attccagtag gcacgagggt
    73441 tggtttcaat tgtttttcat tttctgatac atcttttttc aatccatctg cgactgtttc
    73501 caaccccttt ttccagcctg ctttccagat acttggaatc acgacctctt ctgctgcttc
    73561 aaaatcatag ccagcagcat gatgtgtagc gcgctgcggt agattgatat tttgatcttt
    73621 atatttagaa ataacttcaa atcctctttt tctcaccggt tttcctccct aaataaacta
    73681 ctcctattct aacatatccg aggctgtaaa agactttctt tacaaattct tcaagaaatt
    73741 ctgctatctt aaagtaaacc attaattcgt tctttttcca aacgactgca agttgtacaa
    73801 aaaaactatt ttttacatga ctttactcgt caaaacaagt agaatagaac gaaattaatg
    73861 agtggatatt tgattgaggt gtcacatgcg taaagaaatt aaaaatgcaa aacgaattat
    73921 tgtcaaagta ggaacaagta cattgattta tcccaatgga aatatcaatc ttgcggcaat
    73981 cgatcagtta gcttttgtcc tttctgatct tagaaatcgg ggaaaggaag tgatcttagt
    74041 ctcatcagga gctgtaggcg ttggtatgca taaattacat ttgtcagaac gtccgacttt
    74101 gattcctgaa cagcaagctg cggcggctgt tggtcaagcc gaactgatga atatctataa
    74161 tcaaagattc agtgtatata atcaacagac ggcacagatc ctcgtgacaa gagacgtggt
    74221 agattatcct gaaagtcgta aaaatgtgag gaatacattg gaacagcttt tgattatgga
    74281 cacgatccct atcgtgaacg aaaacgatac tgtagcagtg gatgaattag atcatctgac
    74341 aaaatttggc gataatgatc agctgtcagc catcatggca aaacttgtcg cggcagacct
    74401 tttgatcgtc ctttctgata tcgacgggtt ttattcagat aatccgacta caaatcccga
    74461 ggctcagatg tatcgctata tcgataaaat cgatgaaaag attctggcac aagcaggtgg
    74521 tgctggtagc acatttggaa caggcggtat gcagagtaaa ttaaaagccg cttctaggat
    74581 atttgaagca aacagcgcaa tgattttagc aaatggaaaa aatccggcaa ttatttttga
    74641 tattcttgca ggaaaagaag taggtacttt atttaaggag gacttgaaat gactgacttg
    74701 atgcaattag gacaacaggc aaaagatagc gcgcaacaac tagcacttat gcctacaaac
    74761 cgcaaaaatg agctacttgt tcaaatggca caaaccatca aaaagaatca acaagcgatc
    74821 attgaagcaa atcataaaga cttagaaaaa gcagtcgaaa acaatatcag tgaaacgatg
    74881 caagaccgct tgcagctgac acctgagcga atcaatgcca tggctacaga gatcgaaaaa
    74941 attgcttcgt tagacgatcc tattggaaaa gtagatgaga tgtggacgaa caccgacgga
    75001 ttgagaatcg gcaaaaagcg cgtgccacta ggcgtgatcg gcatcattta cgaatctcgt
    75061 ccaaatgtca cgacagatgc agctagtcta gcattcaaat cagggaatgc cgttatcctt
    75121 cgcggtggca aagaagcatt cttctcgaat caattattag tccaattatt gcagcaagta
    75181 ctggtcagtg ctggcgaatc tccttatgcc atccagtttg tcgatgatac ttctcatgaa
    75241 actgcaggac agttgatgcg attgacagaa tacttagatg tgctgattcc tcgaggcgga
    75301 gcaaaattaa tccaacgagt caaagaacag gcaacggtac ctatcattga aacaggtaca
    75361 ggaaataatc acgtatatat cgataaagaa gcacagttat ctatggcagt gaacatcatc
    75421 gtcaatgcaa aagcttcccg tccatctgtc tgtaacgcgg cagaaacatt gctgatccat
    75481 tctgagatcg cacctttttt cttgccagct attgaaaaag aactggtaga acacggggtc
    75541 tctttacgtg cggatgctcg tgcgttagaa tatctagaga cggctgcttt agcagaagac
    75601 acagattggg atactgaata tctagattat atccttgctg tcaaagtggt cgattctttg
    75661 gatgaagcaa ttgcacatat caatcggcat aatactaaac attcagaaac gatcgtgaca
    75721 gatagttatg cggctagtca acgtttctta aatgaagtgg acgcggcggc tgtctatgtc
    75781 aatgcctcca ctcgttttac agacggatcg gtatttggtt ttggtgcaga gattggtatc
    75841 tctactcaaa agttgcatgc ccgtggtcca atggggttaa atgaattaac ttccacaaaa
    75901 tatatcattt atggcgatgg acaaatccgc gaataatgtt tcgtgtgaaa cattattccc
    75961 gccttttgcc ttttttctgt aaaggttcta tccaactaga aaggtgtcaa tatgtttgaa
    76021 attttaatga ttttttttat ttaccttatt tctctgaata tcgctgcttt tctgggagtc
    76081 ggtattttat cccttttctt tcaattcaaa aaacgaagca tagggagtca acgggaaaaa
    76141 tggtcgcagt attttgacaa aatcgggcaa aaagggctgg ttacacgctt gcacattagt
    76201 tacatggttg cattatgctt attagcgaca atcaattact attctttttt cgatcattca
    76261 atcgcttata cgattacgct actgattgct gggatttttc acttatctta taaatatcag
    76321 ctgaataaaa accacttgaa tcggactttc cgctaaaaaa tatccgtacg ataagtagag
    76381 tgccttgaag atcggcaaaa aactgccgga ctttttagag gctgtgacaa agattttgtc
    76441 acagcctctt tttccatata aacggcgttc gcctaccagc tgcctggtaa atctgtttcc
    76501 tcttcgttaa gaagcgaaaa tccgtcaaat gtttcgccat cataataacg aagcagctca
    76561 tccatggaca agttcatcgt tttttctttt tcttcttccg ctgattcagc cagtagctgt
    76621 tcccgataca cctgatacat accagctatt ttttgctgca tcgcttttct agtttcacct
    76681 tccgcattga ttattggcaa ttctttgata tagccacgat aaatcaaccg atcatctgaa
    76741 aattcaatgg gtaattcaac gagtgtcaac ccgacttcct gaaaatattc ttccagcatc
    76801 tattatcccc ccattctttt tcttaaatga attttagctt acctactgaa aattttccag
    76861 tttcattatt ttgagaaaaa aagggaatgt gacaaaaatc gtgtcacatc ccctttttcc
    76921 gaataaatgg tgttcttctg ccaactcctc aatcataagt cacaatattc aacaaacatg
    76981 agaaactgtt ttttgaaatt gttccttgtg attcagagtt aaacgacagt ctcacaacct
    77041 cttggtaaac ggcgttcaat cagctgaccc tcggtattag ctcaaaaaac ggtaaaatat
    77101 gagaagctat ttttccgttt tgtttctcta atacttagga taaaacgctg attttacaac
    77161 ctctgttaaa cggtgttcaa tcagctgtcc ttcggtatta gctcaaaaaa cggcaaaata
    77221 tgaggaacta tttttccgtt tgtttctcta atactcagga caaaacgctg attttacaac
    77281 ctcctgataa acggtgttca aaagcttgca tctgcggtaa taagcccaac caaacgaaaa
    77341 atatgagaag ctatttttgt ttggctgttc ttatgacttg atgcaggaca gctttttcac
    77401 aacctctgat ttcttattct tcttcatttg tagcaaaata attacggtga taaatgatac
    77461 aacatacact gataagaaca atggcacctg caaaataaaa cgcatagctt tcaccaatat
    77521 gttcaccaat cacacctgca ataaatggtg ctaaagctcc accgaaaaag cggacaaaac
    77581 tatagctgga cgaagcaacg tttcgttgca gacctggtat ttccatagca atcgtcgtca
    77641 acagtgtatt gataattcct tggaaaaatc ctgctaacac aatgctgatc ggtacaagga
    77701 ctggcataga agttccagct cctaataaca gcaaacaaat aatgaataag acaaatgctg
    77761 ctaaaattgt ccgataagtc gtaaacttcc gctcaagagc cggtgcaacg aaaatcgaag
    77821 caatagccag aagcacaccc cagccaaaaa atacaaaacc gacttctaat tcagaatagc
    77881 caatcaataa aaatggcgca tacgcaagta aagtaaagaa tccgaaatta tataacaatg
    77941 cgattagtcc aactgaacga agtttatgat tgcttaacgc tttgacacct tcccaaaggc
    78001 ctacttttct ctttggtttc tcagtttcag gtaaaagtac cgctagggct ataaaaccgc
    78061 aaaacatcaa aaatgctacc ccaaaaaatg gataacgcca tgaaccactg cccaagaccc
    78121 caccgactag agggccaaca gccattccgc agccaagtgc tgcttcatac atcatgattg
    78181 ctttttctgt gttaccaact aaaatactga cgatcgttgc taaagcagta gagacaaata
    78241 aagcattccc gacaccccag ccggcacgta acccaatcag cattccgata ttcccagata
    78301 atccgccaag accagcgaat acaacgatga taaacaatcc tgtcatcaat gtttttttca
    78361 aaccgatccg actagataag aaacctgtaa atagcatcac cacaccagtg actaacatgt
    78421 aactagtaaa taacagggtc gtttcagctg gcgtagcgtt taactgtgca gcaatcgttt
    78481 ttaagattgg gtctactaga cccactccca taaaagcgat catacaggta gctgctacaa
    78541 ctagcgctga acttttcccc tttgtttctt catttgtttg ttcttgtata tctatttgat
    78601 tctccaatat ttccatccct ttctctctat tagcatcata catgatttat tttacatgtc
    78661 aatatgcaca tgtaaaacgt ggaaacttta ctgtgatata ataaagaaaa taagacgaga
    78721 ggtaggattt ttatgaatac tttagcctat atattattat gcgtgctttt atccaaacca
    78781 cgttctgggt atgaattgaa gcagctgatc acaatttttt gggaagccca tcacagtcag
    78841 atctatacaa cgcttgccaa gttagaccag cagggttatg tgaccgtttt ggacaaagac
    78901 gagcactctc aaaaaaagat ctaccacttg actgatgaag gacgtgctct tgtagaggaa
    78961 tggatcaaag aggaaactcc cgctcctacg caaaaagatg aatttctagc aaaaatctat
    79021 gctttttcga cgctcgataa aacaacagct actggactgc tattcactag agaacaacag
    79081 ctgaacaaaa tcatgcaaaa aaatcaaaaa aaattgaatg acctctcgct agacaagaaa
    79141 caagaatttg gacgttatgt tgtgatccag cgcagaatta tgctttgtca gcaggaactc
    79201 caatggtgtc agcaagtaag agaacaaatg cagcagtttt ttgagtaagg gataaacaga
    79261 acctttctag aaatatctat tgaagagcaa attatcttca ataaaagagc aatacagttg
    79321 gaaaagaaaa agcgatccga acgacaggcc aaaacctatc gttcggatca ctatttttct
    79381 agaactgcat tcaagagact atgacaaaaa gcatgccaag gccttttttc aaataacagt
    79441 gttccatcat tcattacttt gaattgcttt tagcacactt cttgaaagcg ggccaacgat
    79501 gatcaactgc aacggtaaag cggcaatcac attcattttc caagcattca tataagcaga
    79561 gaataattga tcaggtattc cttgttccat gatcacaccg aataaagaca tacatgtcac
    79621 cattcctaaa accatcaagc atgaaatagt taagatcatt tggaccttgc tttctggatt
    79681 tactggtagt ttaaaggcaa tttttttcgc cagcacaccg atgatgaaga catccaaaat
    79741 gaaagccacc acgaatccag gcactagacc aatcaacaaa cttgataaat gaagatcttg
    79801 atgcagccat aaattatacg cgctcatccc caagaccatc aaaaaacaca tcattgttgt
    79861 aaaaattatt ccttcttttt tatttgtagg caatctgtac cactccttta aatttgtcac
    79921 gaaaatcatc gtagcacatt taaaaatttc tcgtaagtac tttttttatt ttctgccctt
    79981 ttatttcata aacttcatca gccgtacgct cgacaaagta agaatcatga gaagtaaaca
    80041 aaatcgttcc cggatatatt tgcattagct tctctaaagc ttcgatcgtt gccatatcaa
    80101 taaaattagt cggttcatct aacagcaaca cattacttgg cttagtaaaa agcaaagcaa
    80161 tcgtcagacg ggtcgcttct cccccactta atgtacataa cggtttcgtc aaagtttcct
    80221 gagcaaaccc taagtgattc agcaaactac ggacaagcga ttctgaaaac tctgtttgtc
    80281 tcatgacaaa acgaatgatg gattcttcag atgtcatttg ataacccttt tgctgatata
    80341 cttgaaagct tactttcgga gagagaagga ttccttctcc ttgtttgcgg atatgttcta
    80401 ataaggttgt cttacctgaa ccattttcac cgacaatcgc gattttttta cctaaagaaa
    80461 actgaaaatc accatttacc agtaatgttc tcccacttct ttccaattgg acattttgtc
    80521 ccatgatggg atagcggctg tggagttcaa gagattttgg tactggaaaa cggatttgtt
    80581 tcatctgctg cggcttggtt gtttctccta ttttttggag tcttctctct aatgttttcg
    80641 cattcttttg gatggctttt tgtacactgt ctttttgttt agaggaagac aaccgatcca
    80701 gtctgatttg ttgctttttc tttttactgg acactttttc caacttttcc gcttgttttt
    80761 tcttttcttg gatcgatttt tccaatcgtt ttttctcttt catgatcgac tctgcttctc
    80821 gtagctgagt ctttttctcc aattcctttt gagtgagata ggcactataa ttccccgaaa
    80881 actctcggat ttctccgtct tttacttccc agattttcga tgctagctca tctagaaaat
    80941 agcgatcatg actaacaaaa accagcgtac cataataata acgaagctct tcaatcagtc
    81001 gatcaatact ttttctatct agatgggttg ttggctcgtc tagaagcatc ccttgttcat
    81061 aagaagaaag aagacgtatc aactcgatct ttcgttcttc ccctccgctc catttccgct
    81121 ctgaagtttc ttccagtctt aactggaaat gactggctaa ttccaaattt aggtcctttg
    81181 cttcagcgag ataggttaat tgaggcaggt agttgaaggt gatctctgtt tggattttcc
    81241 catgatcggg aaaaagttca ccggctatta gtttgagcaa agtcgattta ccgacaccat
    81301 tttctcctat gatccctata cgatcttgct gatagacacg aagagaatca atagatagtt
    81361 ctagctggtt gccaaagctg acttgtagat ttgttatatt tactgctaaa ttttccatat
    81421 gttttacact cctatttcat gtaaaacaac aattatcgat actaaaaaag agcagaaaaa
    81481 tctgtttgca aaaaaactgc atcacatctt ttcctactct gaacgaaata actatgacta
    81541 aatcaccaaa tctgtcatag ctttcaccta ttctactgtc gttcgaaaaa atggacatac
    81601 tccatccatg aaattgttgt ttatacatgt cttttgaatt tatttagttg ttattcaaaa
    81661 gttcgaaacg taatttcatc gatgcagtca tttttccgac ctcctttttt attaggttac
    81721 cacacacgat tcaaaaaaag caatagctac tgaatagtaa ctgaaaacct tttattcgaa
    81781 catagtgatc ttttctggtg ctaaacgaac agactcgtaa ccttcttcta ccgcttttcc
    81841 tattgataaa ataataactg gaacataacg gtctttatcc aaaccaaatg cctctgccaa
    81901 ctgatctgct tcgaatccac caattggatt tgtttcatag ccatgatcac gcgcaactaa
    81961 catgaattgc atagccgcta agctagaatc gactttgact acatcgttca tttcttctct
    82021 tgaaaaactc ttgtaataag gaatgatcgc agctaattgt tgatctctga cttcttttgg
    82081 catcttccct gattcgtaag cttggttgta gatttcttcg ccatattcgt aacattgcat
    82141 atcgccaaaa atcatgacca ttgcagaaga agtatcattt tgtctcgtat tgaaacggat
    82201 caaaggacgc aagatttctt ttgcttcctc gctttcagcg accacaaagc gccatggttt
    82261 catattgaca gaagaaggtg cagtcgtcgc ttctttgatc atttccagca tttcttcgtg
    82321 tgggattttg tatgtttcat catatacgcg aacggatctt ctaccagtca taattgttga
    82381 aaaatcattt ttttgttcca ttattattaa cctctttcgt ttctgttgtt atattgcaaa
    82441 gttgggtatt caatttatct aaaagtgtta gaaattgttt catttctaca tcgctgaaag
    82501 ccggggatag cacggaacct gtatctttat ggtccatttc acaatgcttg attttcttct
    82561 gggctttttc tgttagcgct acatacactt cccggttgtt ctgaggattt ctctgacgcg
    82621 aaacatagcc atttgcttcc aataccttca gatgacgagt aactgctcct tgatcaatct
    82681 gcaacttttc ttgcaaactg ctttgtagca atggattttc ttctttcagt ctcatcatga
    82741 tctcgtatct agtaagactt ataccgatat tttcttcaaa gatctggacg attttttgat
    82801 cggctaattt gatttgatat aaaaggtgac taatctcttc taaagacata atttcctcct
    82861 ttctaataat tgactcgtca acgattgact tgtcaataat agaataaaaa aagaaaaaag
    82921 tcaaagaatc agctgtgtaa taaaaaaaac gctctcaata gaaaagtata gagaaaagaa
    82981 attttttgta gaatgaacgg aaaaaatgat aaagaaagca caacgctaaa accaagatta
    83041 ttgattctta tcaaactata gaagatagct cttctgcagt agataatttt acaccgccaa
    83101 aaatttccga aatcgccttt tgcaagaatc ccgtcgaata ttagaaattg gatcagtatg
    83161 cttaaacagt aataaaaaag ttatgacaag atgttgtcac aacctttttt gagtaaacgg
    83221 tgttcaaaaa cttgcatctg cggtaataag ttcaaccaaa caaaaaacat gaaaatctgt
    83281 ttttgtttgg ctgttcttac tacttgatgc agggcagctt tttcactacc tctttttaat
    83341 aaccataaaa tcctattcct tcatatctcc agcccatcgg tgttagccaa tctctttcct
    83401 tagcactcaa ggtatagtga tgggtaccag ttttttcatt tggattaaat agtcgataaa
    83461 ttggcttgga accaccagaa taaaaactta gaccttcata acgccaaccg atttttgtta
    83521 aagagttgat ctcagattgt gccattgtgt aatgatggtc tccactgtaa ggattgtata
    83581 aacggtagac aggttgtccg ctgtttggtg cttgccaagc aatcccttca tattcccagc
    83641 ctacagttgg cagataattt ttttctccgg cattcaatgt atataaatgt tctcctgtat
    83701 gacggttata cagacgatac atcggagaag ttgaagttgg tgctgtatca tactgtgtca
    83761 gattataggt ttcgattaca ttgttcaatg tagaaccata attaggcgca gtggcataac
    83821 gacctgttag ccaagccgta gcatctttgt aagagttcgt attgcttttc catgcaccgg
    83881 aatagtagta aacgcctggc tgaaaagaag tcgtcttcaa taccgttacg ttatcttgca
    83941 atgattcttt gtacgaagga tattttctaa aagcggcatt caccgtaatc cattgcccat
    84001 tcacatattc cgaagtaggc atcgtaacag attggccatt atagcttcct ttaatcccaa
    84061 atagattgta attaggggca gaagccaaag tactgttccc ccagccactt tcaacgatgg
    84121 cttgagcaat catgactgat gcatataagt cattggcact tgccaaactt tgtgcagttg
    84181 aggcaatact atcaatgaac gcttgttgag aattagcaga tgagcgagca taaaaagcat
    84241 agccagtata gttttcgaat tcagacaaat ctgcttgatg ggtatgtgtc aatgtttgcc
    84301 catctgtcaa ttggttcgtg atttcttctg cattctctgg attgttctga gcttctgctg
    84361 atgtactgtc ttcggtttgt gtttgttctg ttgtttcagt tgttggttct tcggttgaaa
    84421 ttgtattttc tgagacagta gctgactgca aagtactttc tgtatcgttt tcaattcctg
    84481 tttctgaaga actcgatgtc tgagttgcga cagtttctgt cgattcttca gctagcactg
    84541 ttgtgttggc aactgctaga tttcctaaaa gaataacgct aaataataaa ttctttgctt
    84601 tcatcttttt tctcctttga attatgaaac ttttcctaaa taacagtgta actaaaaaag
    84661 taaaagcaag tcaatgtcca tatttaaata acatttgttt ctttttaata aatttgcttt
    84721 ttctgcataa aaagacgtaa atgaagaata ttcttctcca ttcacgcctt aaattgatgg
    84781 tcgtttgaat aactatttaa caagtaattc tctctggata ttatccgtta tggattatac
    84841 ttcctgtgtc gtcggacgga taatcatgtt gctgacagac atccggtctg gcgtttctat
    84901 cgcaaatgct acagcagaag ctatatcttc agatgttaat ccccattctt tttgcgcttc
    84961 atgaagttca agagcagcct ttttgtcgct gatggtttga tataattcag tttgaacagc
    85021 tcctggagag atgatcgtag atttgatgtt attttgccgt tgctcttgac gtaatccttc
    85081 catgattgca cggacagcaa attttgttcc gcaataaacg gcggaatcag gataaacaac
    85141 atgtcccgcc acagaatcag ttgaaatgat atgcccagat ttttgttttt ccatgattgg
    85201 aagtacggcg gaaattccat ttaacacacc catgatgttg atatcaagca tattttgcca
    85261 ttcatcacgg tgaccttcaa ctaatggagc ggttggcatg attcctgcat tattgtacaa
    85321 tacatcgatt cgaccgaact tttccatcgc taaatcgata actgcttgga cttgtgcaaa
    85381 atctcttaca tctgctgttt gaatatatag ctcagcttct ggtaaagatt cttttattgc
    85441 ttttaggcga tcttctcggc gtgcagctat aactagttta gccccttttt ctgctaatag
    85501 cttcgttgtt gcttcaccga ttccgcttga agcgcccatg atgacaatca ctttttctga
    85561 taaatcattc attctattct cctcctgttt tgaaaaatct cttgcttata ctcactatct
    85621 cttatttcat cttactgttc aaataatccg ttcgacaggt ttgacgattt tggcagggat
    85681 tcctgcaacg atggtattgt ctgggacatc tttcgtaaca gttgcattcg ctgcaacaat
    85741 ggcattctcg ccaatcgtta cgcctggcag aatagttgct cctgctccga tccaagcatt
    85801 tttcttgatg tggattggct ttactgacaa gcctcttcgt tcttttggat cggttagatg
    85861 gttcaccgtg atgaggttga cacgaggacc aatcaatact gaatcttcta aacagatgcc
    85921 acctaagtca acgaacatca cttgacgatt gataaagaca tttttcccta tactaatatg
    85981 tttgccgaaa tctgtataaa aaggcagcag aacagtcacc gtatcatcta ctttgtcact
    86041 aatgatattt tctagccgtt tgcggatatc tgctggttca tggtacccag tattcagcgc
    86101 tgctaaaggc tcttcgtttt ccgcttggat ctgatggatc acttcaaaga gcggatccgt
    86161 tactcgaatc tctttattga cgatttgttt ttcgatgagg cttgtcattt tgctccctcc
    86221 ttttatcctt actttataac ggacggctta tgatgtctaa tacttataaa acatacttaa
    86281 ctattccttt taactatact tagtggtatg cttttttaaa aacaggaggg aaaaaatgga
    86341 tattcgtata ctgacgtatt ttttagcagt agctagagaa aaaaatatca gccgagcagc
    86401 aaaatctctg catatcacgc aacctactct ctcaaaacaa ctgaaagatt tagaagaaga
    86461 acttggtgtg acacttttca ctcgaggctc ccgagagata cagttgacgg attctggtcg
    86521 atatctttat tcaaaaggaa aagaaatctt gtcgttggta gaaaaaacgg cgaacaattt
    86581 aacaaatgac gcaatcatct ctggagaaat ctacattggc ggaggcgaaa ctaaagcaat
    86641 ctctttcatt tctgatagcc ttcattcgtt gatcaaggcg catcctgata tccagtttca
    86701 tttatatagc gggaatgcag atgatatcat agacaaactg gatagcggat tacttgattt
    86761 tggactcgta atcgaaccga caaacaaaca aaaataccag tcatttcgac tgccagccca
    86821 agatcgttgg ggacttttga tacatcggaa tcatccttta gcaaagcaaa cagtcatcca
    86881 tccgcaggaa ctgaaaactg ttcctttgat catttcccgg caaacgtcag tggacagtca
    86941 actagctgaa tggctaggtt tcagcataga tcagttatcg atcgtcggaa catataactt
    87001 attgtacaat gcttctttga tggtcgataa cgggactttt ggtgcattag ctctagatgg
    87061 gatcatcaat acttatggga cggaaaaagt attcatccca ctcgatccgc cattacaagc
    87121 ttcactgaat ctcatttgga aaaaagacca gcctctttct cctgctgctc gtaaattttt
    87181 agatgtccta acggagaaat acagttaagt agagtttttt tccttcgctt tcagcttttt
    87241 aacaacgaat aagttaagca aaagatccaa tgattaaata acataagttt cttaatttgt
    87301 tccttccttt tatttctgat aattccagta aattactata attataaaaa aagaattgac
    87361 aaaacataac tgtctatata aaattgatta cgttaacaat aattcgtttc atgtgactag
    87421 atcatactag gcatgaaaaa tctctgagga acatggtttc cctaggaggt ttttcgtgtc
    87481 tatttttgtt tggccgcaat caactggtct agcaaacgtg aaaaccaaat cttcaaaaag
    87541 ggtgcgcgca tgaaaatcaa aaagatcttt aatcaaaatg cagtattggt agatgacggt
    87601 ggacaggaaa aagtagctat tggaaaaggg attggatttg acagaaaaag aaatgatttg
    87661 atttttgatc gtgatgtcga acggatattt gtcatggaac cagaaggtca aatcaagttg
    87721 caaaatttac ttaatcagat tgatgaacgc tttttatttg cagcagaaac gatcattgat
    87781 catgcggaaa ctgttttgat ggaaaagttg aatgaacatg tattgatcgc tttgaccgac
    87841 cacattgctt tttctgcgga gaacattaat aacggtatcg tcatccgcaa caaactttta
    87901 agagaaatcg aagtgcttta cagtgatgaa ttcagtattg ctcaatgggc agtagagtat
    87961 ctgacaaaag agcttgggat tccatatact tatgacgaag caggatatat cgcaatccat
    88021 attcacagcg gacgaagcgg tcggaacaat aaccatcgca gtatccgtga agtgacgatt
    88081 gtatctgaca tcatccattt agtggaatca gaattggaaa cagatatcca ttcagaacca
    88141 ttttcactga attattcaag actggtgaat catttacgac tgcttctaca acgaacccat
    88201 gcgcaacaat atgctgtttt agacacggaa atcgtagaaa tggtcaaacg aaaataccca
    88261 gaaagctata agatttctaa aaaaatccgt gtgttgctga ccaaagaata ccagctcgcg
    88321 atcacgaaag aagaactggg ttacttggcc atccatatcg agcgtttaag aagcgcaatc
    88381 gtacaaacaa atgttaacaa tcatgaggag gaaaaaaatt gaaagcatat ctacaacgaa
    88441 tgggacgttc ccttcaactt ccagttgcgg tcttacctgc tgctgcttta ctagtaggga
    88501 tcggaaactg gtgggcatct tatagtaacg acattgtcgc tcatttttta caagctggcg
    88561 gtaacgcagt tttaggtcaa ttacctttgc tgtttgccgt tgggttagca ctagggatgt
    88621 cagtggacaa atcaggtgct gctgccttgg caggtttggt cgcatttgaa gttccagtaa
    88681 atgttttaaa aactgattca gtagctactt tattgaatat caaaccagaa tcagttgatc
    88741 catcttttgc tcagatcagt aacgtattta ttggtattat tgccggattg attgcggcag
    88801 ctctttataa tcgtttccac gaagcaaaat taccgatggc tttatctttc tttagcggca
    88861 agcgtctagt gccaatcttg gctgcctttg tcatgttgct gatcagtgca gtgctactgc
    88921 tcgtatggcc tccggtttac tctgctttag tttcttttgc taaattcatt ttgagtttag
    88981 gctggggtgg tgcaggtctt tatggcttct tcaaccgttt actgatccct acaggattgc
    89041 atcaagcaat gaactccgtg ttctggtttg acgtagcagg aatcaatgat attggtaaat
    89101 ttttggcttc tgaaggaaca aaaggcgtaa caggaatgta tcaagctgga tttttcccaa
    89161 tcatgatgtt cgggcttcca gcaggagcat atgctattta cagaaatgca cgtccagaaa
    89221 acaaagcaaa aacagcttcc ttgatgttag cagcagcttt tgcttcattc tttactggtg
    89281 taacagaacc acttgaattc tcatttatgt tcgttgcttg gccattatat gtcttgcatg
    89341 caattttcac tgggatttct ttagcagtga gtgcattctt ccattggaca gcaggatttg
    89401 cctttagtgc cggatttgtc gattacttcc tatctttgaa aaatccgatt gcgaatcagc
    89461 cattgatgtt gatcgtccaa ggattagtaa cagcagtgat ttactacttt ggctttaact
    89521 ttgctatcaa aaaattccat ctgatgactc ctggccgcga ggaagcagat ctttcagatg
    89581 acgatactgc aacgactaat tcagacaata aatatgcggc acaagcaagc aaaatctacg
    89641 ctgcattagg cggagccgac aatgtaactt cgatcgataa ctgtactaca cgccttcgtt
    89701 tgcaagtcaa agatacaggc ctgatcgatc aaaataaaat caaagccact ggcgtacctg
    89761 gtatgaagat catcgacgga aaaaatgcgc aagtcatcgt aggtacagaa gtacaattcg
    89821 ttgcagatga gatggcaaaa ctacatggtg gtgctgcaac acgacctgca caaacaaaca
    89881 ccgtggttaa aacagaaaca tttgccggtg gaacgatcga acaagatatt tacgctatcg
    89941 caaatgggaa actgattcca atcactgaag tttcagatga tgtattcgcg gaaaaaatga
    90001 tgggtgacgg ctatgctgta ttaccagaaa atggagaaat cttctctcca atcgctggta
    90061 cgatcacaaa cattttccca acgaaacatg ctgtcggaat ccaaacagat gctggtatcg
    90121 aagtcttgct tcatatgggc atcaacactg ttgacttaaa aggcgaacca ttcactttat
    90181 atgtaaaaga aggtcaaaaa gtcgcaagag gtcaattgat tgctttagtt gatcttgccg
    90241 ctattcaatc tgcaggcaaa aacacggata tggtcgttgt attcacaaat ggcgacaaag
    90301 tccaaagctt agaaatcgaa cccgcacgtg atgtaaaagc aaatgataaa atcggctctg
    90361 tttccaacaa agcctgagcc atcgtcttcc ccagactttt ttatcgataa acaaacagga
    90421 gctgagacaa aaatcttgtc tcagctcctt tttccgaata aaaacggcgt tctcctgcca
    90481 actccgcggt aataagtcca gccaaacgta acatatgaag agctattttt tatttgactc
    90541 ttcttattac ttgatgcaga acagcttttt tacaacctct gataaacggt gttcaaaaac
    90601 cagtttatcg gtatgaagtc aaatttatgc aaaaatgtgg aaagccattt tcacaaaatt
    90661 tttcttcata ctcaaaacta atcggctttt tcacaacctc tttatacatt attcctgatc
    90721 caatatttct tttaatgctg cttcaagaga agttaatggt tttcccaata ctttttctaa
    90781 atcatcagag gaaatagcta actgatcctt tttaatatca tgttggatcg ctaaaaacat
    90841 gtcagctgct tcttgaggga atccggcttc ttttagattt tcgacaaacg ctgcatcact
    90901 tgcttcaata atttctaact ttttacctgt cgcacgctct aatgccttgg ctaattcttc
    90961 atagtcagtt agattgccgg ataattctaa gatttctgga gagtcacttt ctactagtac
    91021 ttttgctgca gcttctgcat attcacgttt caatgcccaa cctgtttttc catttcctgc
    91081 agcataaacg aattgaccag tttttaaagc ctgcccaatg ataggtaatt cattctctac
    91141 ataccaatta ttccgtaaaa atgtatggga aatacctgaa tccttaatca ttttttcagt
    91201 gaattggtga tctggcgcta atacactgat cgagtgatcg gcatctgcaa aacttgtata
    91261 agcgatataa gataccccag ctgcttgtgc tgccttcact acattcttat gttcggcttg
    91321 acgatttcct ggaacgccag agacaaaaag caatcgatct attcctttca acgcttcttt
    91381 cattgagtct aaatcatcat aatcaccaat acgtatatta aatccagctt ctttcagtgc
    91441 ttctcctttt tcttcactac gtactagtac atagatttca ttttgtggaa ccatcttttt
    91501 taagaattcc agcgcataac caccaaaacc tccagttgct cctgttacta aataatgcat
    91561 atttgccact ccctattctt tttatttgtt aagatttaca gctgtacatt tataaattac
    91621 acctttatca taaagagtgt ggtaagataa gtcaagcaaa aaaaatgaaa ggatgacaac
    91681 tgatgaagta ttctctgcaa tttagtgatg caatccatgt acttgcctat attgaaatgt
    91741 ttcaagatac ccgatcactt tctagtgaga tgattgccag tagtatcgaa acaaatgcag
    91801 caaatgtacg taaaatcatg agtcagctca aaaaatccgg tttgatcttt acaaaaaccg
    91861 gaaaaccgca gccttccctt gccaaaagtc cagatcagat ttctttacta gatgtctatc
    91921 gcagcatcga aggtaataca cagcttatcc aagtcgatcc aaaaacaaat ccaaattgcg
    91981 tcgttggtgc gaatatccaa gaagctttag aagaaagcta cgctcggctg cagaaaaagg
    92041 cagaagaaga aatggctaag atcacacttg atagtctgat acataaaata gccgttttgg
    92101 aaaaagaaaa aagaccagaa aacacaggcc tcgtagagag atttttataa ttctctctac
    92161 tgtgcctgca tttttctgat cggaatcgtc tacataaatt cttttattct ccgggggaca
    92221 aaagccttgt catgcccctt tttcctctct aatgctcagg tcaaagcgct gaagtcatga
    92281 cgtcttgata aacggtgttc aagtagctga ccctcggcat tagttctgaa aaatgcaaaa
    92341 tatggaaagc tatttcccat ttttcctctc taatgctcag gtcaaagcgc tactttcacg
    92401 acctctatat tatcatggtt tattgactac cggtatccag atttctccca tatatttccc
    92461 gttggtaact cccatttcga ctacagcatt tggaccccca acataggcgt aatcttctac
    92521 ttctcttaaa acatgcccaa aggccgtatt cgtcaaatca tttcttaatt cttcaggcga
    92581 ttctgctaaa gcgattacaa caagatacgt tccttttggg aatgggatga ttcgggtagt
    92641 gtttggaaca tcctgactcg tttgaacacc gacatagtac atcattttcc cgttataggg
    92701 ttcgttgact gtaaagaaat aatgattttc agcagcttgt ttcaatgcat ctattgttcc
    92761 attaccgata actttttgct caaatgcttg tttctctgca gccaaaccga caaaatccgt
    92821 attgctactc ttcaattcca caccaatacc cgtcaccgta aaatctggtt tttcttcgat
    92881 cctatacttt gccatttctc ccaccctttc tattatatta agttcatctt acataaaatt
    92941 aaagggaaat gacacgatta cgctcttttt tctagggata tttttatttt ttaactattt
    93001 ttttgctttc actgcaatcc aaatctcact gtaaacatta ctgaaatcct ctccccattt
    93061 tgtaaaagag atctcgggag cttcgactaa ctcataatct gaagaaggaa gccattctgc
    93121 atagattttc ccccaagtat cttgtaaagt ttggggaaat ggccctttat taggaaaaat
    93181 ggcccaagtg tgttcttcta tagcaagcat ttctaaatca gcgtacggat tttcttttgt
    93241 cgtagcaaag ccaatcatgt gcatcaaact gcccttttct tccaagcggg ctccatcaaa
    93301 tccataagaa acgttgatta cttgatttgg atataaatct cctaacttat gcatctcttc
    93361 cctttgagtc tccgtgatcg tctgcgctaa cttttgtatt tcttgattga ctccttcaaa
    93421 tttgacagga acttttttcg ccacacctgc cacataaaac tgttctttct tttcgatacg
    93481 aaattccatc gattttcctc ctctgacatc aataaaaaat gtcaagcgtg gaaaagattt
    93541 ttgtatgccg tatctgctga tttctgaagg cagatatcct gaccaatcac gaaaagctct
    93601 tgaaaaacct tcgactgttt catatccata tctaaacgcc gtttctgtca ctcttgatcc
    93661 atctaggaga tcgcgattag ccaaagccaa tctacggttt ttgatatagt ccattagcga
    93721 catccctgca ataaacgtaa agatgcgttt taattgatat tcagagattc ctgtattttc
    93781 ggctattatt ttataggaaa gtccttctgt caaatgttct tcgatgtaat cgatcaattg
    93841 gttcagatct ttaagcatat ttatcttcct ccttgcttgc ctttttatca tattatcaaa
    93901 gaaagtcaat tacttgatga atcaggaacc aatttagtca gatgtagaaa atgttgtatg
    93961 gatctaaaga aaattctcaa gcagcgtttc gtaacggata attggtatta tctgcctatc
    94021 tatgttttac ctttagaaaa ataaattttg gggaagacac attctaagta aacaaagcgg
    94081 taaaaatatc ttttattccc ttagttaatc cgcttactac ctactgtaca aagcaaattg
    94141 ttgttcaaac tgctgatcag aacgctgctt ggatttttct ttgcgataca tcatacctct
    94201 tttatcaatt ggaacaatta tttatttcta acaaaatcac ttcatttttg tcagataaat
    94261 aaaa
//
LOCUS       DACDKM010000002        82558 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20834_2016
            SAMN12640370-rid9891663.denovo.002, whole genome shotgun sequence.
ACCESSION   DACDKM010000002 DACDKM010000000
VERSION     DACDKM010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640370
            Sequence Read Archive: SRR10040891
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 82558)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 82558)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742835.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742835.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:04:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,012
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 29 of 75
            Pseudo Genes (incomplete)         :: 33 of 75
            Pseudo Genes (internal stop)      :: 19 of 75
            Pseudo Genes (multiple problems)  :: 5 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..82558
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640370-rid9891663.denovo.002"
                     /isolate="E20834_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            48..437
                     /locus_tag="IV334_000087"
     CDS             48..437
                     /locus_tag="IV334_000087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase"
                     /protein_id="HAP8942193.1"
                     /translation="MSPYYAQGNLIYVRPKNTNEILVGDIITYYENSGKKISTRRVIA
                     VENNHEDFYTKADTKTYIEPGVVKKRNIIGSPIFQIPYIGLVLSKTAFAWIQGLFFTI
                     AFCLTGITAWNTFVELKKKRRRTQKFL"
     gene            565..1248
                     /locus_tag="IV334_000088"
     CDS             565..1248
                     /locus_tag="IV334_000088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-methyltransferase"
                     /protein_id="HAP8942194.1"
                     /translation="MRNEMMHRPVVKEELLDFMRTQQKKLPGELGKLEEEAHVAEVPV
                     IPHETVVFLKFLLGQLQPERILEIGAAIGFSSSVMATTIGENAHVTTIDRFDVMIEKA
                     KKNYERLGLTDKVTLLEGQAADILPELSGPYDFIFMDSAKSKYIEFLPECLRLLRKGG
                     VLMVDDIFQGGTILLPDEEIPRGKRAIHRKLNEFLRVVMDHPDLTSTILPLGDGVILM
                     TKESETIEL"
     gene            1557..1630
                     /locus_tag="IV334_000089"
     tRNA            1557..1630
                     /locus_tag="IV334_000089"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1591..1593,aa:Arg,seq:tct)
     gene            1789..2148
                     /locus_tag="IV334_000090"
     CDS             1789..2148
                     /locus_tag="IV334_000090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1304 family protein"
                     /protein_id="HAP8942195.1"
                     /translation="MTILSRILVTLVAIEFFYIMYIETVRTDSDTTSRVFKMSKEELS
                     RKSVQTLFKNQGVYNGLLGVGLLYGAYLSSASKEITSMLLISIFFVALYGSLTSDRWI
                     LLKQGGLALLALLSIFL"
     gene            2271..3902
                     /locus_tag="IV334_000091"
     CDS             2271..3902
                     /locus_tag="IV334_000091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HAP8942196.1"
                     /translation="MFKNYVSSLKKEFSGYDLSRFQKDLLAGITVAAVALPLALAFGV
                     SSGADAAAGLITAIIAGIVIGGLSGGFYQISGPTGAMAAILMSIAATKGMQGVLLATF
                     LAGLFLLAAGLFRLGTLTSFIPAPVITGFTSGIAIIIALGQIDNLFGVHSEGTNVIEK
                     LSSYQTIGFPINTASLIMGGLVILGMFIYPKKWAKRMPSSLLAIILATAVMLLFHLPV
                     ATVGKIPQTLISSNRLNMGDFSFSALQQVAVPAISIALLGMIESLLCGASAGRMANKP
                     LDSNQELMAQGIGNLLLPFFGGIPATAAIARTSVAIKSGAQTRLAGMIHAVVLLLSML
                     VFAPVMSQIPMPALAGVLIVTAWRMNEWETIKELFTNKYWSAVLLFILTMICTVIFDL
                     SIAIVIGVIGGCVFFVVKSAAITISLETIDWQRMGLPETEKLNNWTVVYISGPLFFMS
                     SEKLKNKLEELNHHDGVIFSMRGVPSIDLTARAVFDEFQEKAEQRGQTIIYTSLQPEV
                     EKQLSHLWEHTFTHQHQTVAHALTALHAKYTAISH"
     gene            complement(3964..4605)
                     /locus_tag="IV334_000092"
     CDS             complement(3964..4605)
                     /locus_tag="IV334_000092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HAP8942197.1"
                     /translation="MEYPKELIQEASLRMNGRPVEGFIAGQGPLHPKLMLVGEAPGKT
                     EIDTHVPFSGQAGKELMQALSSTGLTREEVYITSAVRSRPYRVTHRINKRTQQSETVY
                     PNRTPTRSEVFAHAPILDYELMKVQPKLIATLGNIGLQRLLGKEYTVTKNHGQLYTGP
                     VLQLTEQKDAYEWSEKNYRILPLFHPAAIFYNRSLVPVIHQDWQVLASLLQQY"
     gene            4887..5195
                     /gene="rplU"
                     /locus_tag="IV334_000093"
     CDS             4887..5195
                     /gene="rplU"
                     /locus_tag="IV334_000093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002355866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HAP8942198.1"
                     /translation="MYAIIKTGGKQVKVEVGQAIYVEKLDVEAGEKVVFDEVILVGGE
                     STKVGAPTVAGATVEGTVEKHGKQKKVVTFKYKPKKHSHRKQGHRQPYTKVVIDAINA
                     "
     gene            5222..5566
                     /locus_tag="IV334_000094"
     CDS             5222..5566
                     /locus_tag="IV334_000094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal-processing cysteine protease Prp"
                     /protein_id="HAP8942199.1"
                     /translation="MIKGTFKRNDAGQIVSFTLTGHADAGPYGSDIVCAGVSALAIST
                     VNGIASLAGFEPIVEMNEEEGGYLYTEVTSGMTQEQNNIAEILLENLLLGLQSIEAEN
                     SEYIQIKTINDK"
     gene            5579..5872
                     /gene="rpmA"
                     /locus_tag="IV334_000095"
     CDS             5579..5872
                     /gene="rpmA"
                     /locus_tag="IV334_000095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HAP8942200.1"
                     /translation="MLLSMNLQLFAHKKGGGSTSNGRDSESKRLGAKRADGQTVTGGS
                     ILYRQRGTKIYPGVNVGIGGDDTLFAKVDGVVRFERKGRNKKQVSVYPVAQEA"
     gene            6279..9113
                     /locus_tag="IV334_000096"
     CDS             6279..9113
                     /locus_tag="IV334_000096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAP8942201.1"
                     /translation="MAKRIDRIYMYVKEKTAHLTKAEYDQGVTTQEIAEVLGIQRTNS
                     SKDLNQLVREGKLIKTDGRPVRYIYQTLVTHVKPVTKHVVSYKETPVSEEKNTLQIDT
                     KDIFAKIIGANGSMKNSVEQAKAAILYPPRGLNCLITGPTGSGKTYFAHAMYHFAKAH
                     HVIDEETELIVFNCADYANNPELLMSHLFGYAKGAFTGADEEKMGIIDQADGGMLFLD
                     EIHRLPPEGQEMIFYFMDHGTYSRLGETTKSHEANVRIVGATTEDPGSSLLETFVRRI
                     PINIKLPAFEKRPANEKVDLVKIMIAHEANRTQRKISLTEDVAKALIGSVTYGNIGQL
                     KSNIQLVCARGFLNHMQSPEISITIDDLTEGIRSGLIQLASNRSAMAELSKILEPKIT
                     VAPNDTIMKIQSDSYELPYNLYDIIGDKAALLKSDGLDQEAINHFISTDINIHLKSFY
                     KDHGFSFNADSKLAEFVDPKIIEVTNQIYVMVKRSLPYEFQQNFIYAMSLHISSFLKK
                     INLGEERHTNDNIREMAFDFPDEYAVAKEVRSYIETYFQVEIPESEDYYLTVLLVSLR
                     ANQASGRIGVVVAAHGNSTASSMVQVVQQLLDAENVRAVDMPLDMDPKTALDRIERNV
                     QEVDEGSGVILLVDMGSLASFNSQIQRDTGIPVRTVDMVTTSLVLETVRKVSVLGTDL
                     DMLHESLKNFRGYAEISSVESVQEAKPIHKKKAVLAICASGEGTAQRIKELIERAVNK
                     RQETELEVLALSIVEVKEELPVIQKEYQIIATTGITDLNIQAPFIPLERFIDQNIEVI
                     LDQLLMESELEETEFISLDEESAKNTCVEFISDNFIFINGSKLIDPMWQFSTQISQTT
                     GIGDEEYGFKINLVMHTAGMIERIIRNEPLTVEENELTNTTNDPLYSQLAASVVLLED
                     QIKVKVPIEEMYYLLRLVHNQLDKKEYTVP"
     gene            9304..9795
                     /locus_tag="IV334_000097"
     CDS             9304..9795
                     /locus_tag="IV334_000097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit IIB"
                     /protein_id="HAP8942202.1"
                     /translation="MDIRLARIDDRLIHGQVATVWSKQTGVERIIVISDVVAQDKLRK
                     FLLKEAAPPGITANVITVQKMIQIYHSGLLSRAKVMLLFTNPQDVEAVVRCGVQLKSL
                     NIGGMSFTEGKSMITNFVSVNQQDIDSFRYLNSQEVELEIRKVPADRKILLMELLAKI
                     KKV"
     gene            9898..10887
                     /gene="manX"
                     /locus_tag="IV334_000098"
     CDS             9898..10887
                     /gene="manX"
                     /locus_tag="IV334_000098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIAB"
                     /protein_id="HAP8942203.1"
                     /translation="MVGIILASHGQFAEGILQSGSMIFGEQENVKAVILKPSEGPDDL
                     RAKLEEAVASFDNQDEVLFLVDLWGGTPFNQSNTLFEEHKDKWAIVSGLNLPMLIEAY
                     ASRFSMESAHEIAAHIIETAKDGVKVKPEELEPAEAPKAAVEDAQPKGALPEGTVVGD
                     GKIKYVLARVDSRLLHGQVATAWTKAVQPNRIIVVSDAVSKDDLRKRLIEQAAPPGVK
                     ANVIPISKMIEVAKDPRFGNTKALLLFENPEDVLTAVEGGVDIKELNVGSMAHSVGKV
                     VVSKVLSMGQEDVEAFEKLEQKGVKFDVRKVPNDSRDNMDDILKKAKAELAKA"
     gene            10920..11723
                     /locus_tag="IV334_000099"
     CDS             10920..11723
                     /locus_tag="IV334_000099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_463630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter subunit
                     IIC"
                     /protein_id="HAP8942204.1"
                     /translation="MSIISIILVVLVAFLAGMEGILDEFQFHQPLVACTLIGLVTGNL
                     EAGIVLGGTLQMIALGWANIGAAVAPDAALASVASAIILVLGGQGVKGVPSAIAIAVP
                     LAVAGLFLTMIVRTIAVPIVHLMDAAAEEGNIRKVEMWHIIAVCLQGVRIAVPAAALL
                     FIPAQTVQSFLESMPAWLTDGMAIGGGMVVAVGYALVINMMATKEVWPFFVIGFVVAA
                     ISQLTLIALGALGVALALIYLNLSKMGGSSNNGGGSNSGDPLGDILNDY"
     gene            11744..12658
                     /locus_tag="IV334_000100"
     CDS             11744..12658
                     /locus_tag="IV334_000100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter family
                     subunit IID"
                     /protein_id="HAP8942205.1"
                     /translation="MAEEKIKLSKKDRLAVAWRSTFIQGSWNYERMQNGGWAFSMIPA
                     IKKLYKTKEERSAALKRHLEFFNTHPYIASPILGVTLALEEERANGAPVDDVAIQGVK
                     VGMMGPLAGVGDPVFWFTVRPMLGALGASLAMGGNILGPIIFFLGWNIIRWAFMWYTQ
                     EFGYKAGSKITDDLSGGLLQDVTKGASILGMFVLAALVQRWVSIKFLPVVSTVKLDKG
                     AYIEWDKLPSGGEGIKSAFEQVNNGLALSPEKVTTLQDNLDQLIPGLAALGLTLLCMW
                     LLKKKVSPIVIILGLFVVGVVGHVIGLL"
     gene            12741..13115
                     /locus_tag="IV334_000101"
     CDS             12741..13115
                     /locus_tag="IV334_000101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF956 family protein"
                     /protein_id="HAP8942206.1"
                     /translation="MVQSINTKVDLVIDATSFTGLTDYGQIMIGDNGFEFYHARDRRK
                     YIQIPWEEVDYVLASVMLKGKWIPRYAIQTKKNGTYTFSSKEPKKVLRAIREYVDEDR
                     MVQSLSFFDVVKRGIRSMFKRK"
     gene            13254..13499
                     /locus_tag="IV334_000102"
     CDS             13254..13499
                     /locus_tag="IV334_000102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HAP8942207.1"
                     /translation="MSWLWTLIVGAVIGAIAGAITSKGNSMGCVTNIIAGLVGSSIGQ
                     AIFGEWGPKAAGMALIPSILGAVILVAVVSFFFGKRS"
     gene            13671..14735
                     /locus_tag="IV334_000103"
     CDS             13671..14735
                     /locus_tag="IV334_000103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HAP8942208.1"
                     /translation="MMLRVEKLRKKMQEENLDSFLVTSPYNLRYLTNFTGTTGLAVIT
                     LEKAFFITDFRYTEQAAAQAQGFEIIKNVGPIFDEVANLVRKEELHSLAFEETTVSFL
                     EYSVLEEIIDAELVPVSQMIEELREVKDEEEIAIIEKACHIADMAYDHILKMIQPGMT
                     EIEVANQLDFYMRSLGASGVSFDTIVASGLRSAMPHGVASKKIIEQGDLITLDFGCYY
                     EGYVSDMTRTFAIGDPGEKLKEIYQIVLDAQLAVIDAAKPGMTGVQLDAVARDYISKH
                     GYGEAFGHSTGHGIGLEIHEGPNVSSRAEKQFVPGNVITDEPGIYLPGIGGVRIEDDL
                     LITEDGNRVLTRSPKELIIL"
     gene            14981..15406
                     /locus_tag="IV334_000104"
     CDS             14981..15406
                     /locus_tag="IV334_000104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HAP8942209.1"
                     /translation="MADEKNLILNANQDLGEIVIAPEVIEVIIGIAASKVEGVYGMRG
                     TFASNVTELLGRAAHGKGVYLANEEDGLKVDLYCYLEYGVSVPKVAMEMQERVSQQVL
                     FMTDIDLSEVNIHVVAVVPEKLEQPTIEELFEDEEEENE"
     gene            15399..15848
                     /gene="nusB"
                     /locus_tag="IV334_000105"
     CDS             15399..15848
                     /gene="nusB"
                     /locus_tag="IV334_000105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HAP8942210.1"
                     /translation="MNKELSRHEIREMALQALFPLDFNADLTKEDAIFNAIELDHRDM
                     INEDESEFVPVYLDTLVGGVCAKKDELDKVIEKHLKNNWHINRISKMDLVILRIAIFE
                     MTYVTDVPAAVALNEAIELAKTFSDDRSRKFVNGVLSNVLKELEAGA"
     gene            15945..16799
                     /locus_tag="IV334_000106"
     CDS             15945..16799
                     /locus_tag="IV334_000106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase"
                     /protein_id="HAP8942211.1"
                     /translation="MAELINGKELAEKMQAEIAEKIQKLKDNGIHPGLVVLLVGENPA
                     SQIYVRNKERVAKEIGIHSLVERYPETISEDELLAEIEKYNQDPSFHGILVQLPLPKH
                     IDEEKILLAIRPEKDVDGFHPMNLGRLLAGKPDKIPCTPYGIMKMFEAYSIDPAGKRA
                     VVIGRSNIVGKPMAQLLLMADATVTVAHSKTENLAELAQEADILVVAIGRGHFVTKEF
                     VKPGAVVIDVGMNRDANGKLIGDVKFDEVEPIAGWITPVPKGVGPMTITMLMYQTVKS
                     AEAFGAGE"
     gene            16800..18137
                     /locus_tag="IV334_000107"
     CDS             16800..18137
                     /locus_tag="IV334_000107"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HAP8942212.1"
                     /translation="MAQEYLTVTTLTKYLKRKFDADPYLERVYLTGEISNFRLRANAH
                     QYFSLKDDHAKISAIMFKGAFQKLRFQPKEGMKVLVVGRISLYEAGGSYQIYVEHMEP
                     DGIGALYQELEERKEKLMKEGLFQGPKKMLPRFPKRIAVLTSPSGAVIRDIITTVKRR
                     YPIVQLVLFPTVVQGNQAADNVVRNIQRVEEMGNFDTMIIGRGGGSIEDLWPFNEERV
                     ARAIYQANTPVISSVGHETDTTIADLVADVRAATPTAAAELAVPVLSEELMRIQERQA
                     RLQQAFVRQIQRKQERFERARRSYVFRQPERLYEGQTIKLDQLKQRLYQATQQIYHAK
                     EKQTIALSHQLEQVAPLYRVKAARQETAYLEKRLEEKIQQYMKQQQQRFAQAVQSLDL
                     LSPLKIMGRGYSYTTLNDQVVKSVTEIHQGDLLTIHYQDGIVKGQVQEIDKGE"
     gene            18142..18366
                     /locus_tag="IV334_000108"
     CDS             18142..18366
                     /locus_tag="IV334_000108"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HAP8942213.1"
                     /translation="MENPTFEESLQELEKIVMQLEQGDVPLESALDSFKRGMELSKHC
                     QDTLAKAEETLTKMMTESNEEVDFDGNEES"
     gene            18368..19258
                     /locus_tag="IV334_000109"
     CDS             18368..19258
                     /locus_tag="IV334_000109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HAP8942214.1"
                     /translation="MRLTDFSAIHLPAVEKEILSFLDEHTTDRGLYDAMSYSVKAGGK
                     RIRPLLLLTAVASFDEPIDVPVYQVAAALEMVHTYSLIHDDLPAMDNDDLRRGKPTNH
                     KVFGEALAILAGDGLLTGAFQLISMAHLGNSPKLLLLQQLAVCAGSQGMVAGQAADIE
                     GESKKLSLEELAFIHERKTGHLIRYALLAGGILAKQPEEILLLLQRLAEHLGLAFQIR
                     DDLLDVIGTTKTLGKTAGKDERMEKNTYPRLLGLEKTREALEIELLSANKIIDKLEED
                     VFSFDGGLMRQMIKQFDVEK"
     gene            19267..20085
                     /locus_tag="IV334_000110"
     CDS             19267..20085
                     /locus_tag="IV334_000110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TlyA family RNA methyltransferase"
                     /protein_id="HAP8942215.1"
                     /translation="MEKERVDVLAVKQGLFETREQAKRSVMAGLIYNEKNERFDKPGE
                     KIPVSSELRVKGKKLPYVSRGGLKLEKALKAFDLSVEGKTLLDIGASTGGFTDAALQN
                     GAKMSYALDVGYNQLAWKIRQDPRVVVMERVNFRYAKPEDFTLGIPEVAVIDVSFISL
                     KLMLPPLHAILKEEGEVIALIKPQFEAGREAVGKNGIVRDPQTHQKVLEDILSFAAQG
                     GYDVLELSYSPITGGEGNIEFLAHLRKVPESGTINSAINMAEVVSNAHEQFDHK"
     gene            20192..20641
                     /locus_tag="IV334_000111"
     CDS             20192..20641
                     /locus_tag="IV334_000111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HAP8942216.1"
                     /translation="MRKKDRHRLITRLLSEQDIRKQEEFVEILKSKGISVTQATISRD
                     IKELKLIKVPASDGGYRYSIPAETNEDVNTKLEKLLKDAFVAVDQMEKFVILKTLPGN
                     ASAAANLIDKRYKKELFSIINDDDNVLMITRTEDQAVLLKKDFLHYL"
     gene            20659..22341
                     /gene="recN"
                     /locus_tag="IV334_000112"
     CDS             20659..22341
                     /gene="recN"
                     /locus_tag="IV334_000112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HAP8942217.1"
                     /translation="MLQEISITNFAIIPELRLSFHEGMTALTGETGAGKSIIIDALGL
                     LAGGRGSSDYIRQGAEKCVLEGLFELPKQEGFSELMVELGIETDEDNLIVRRDMSLTG
                     KNVCRVNGHIITLANLRRIGSYLVDIQGQNEHQELLQPESHLALLDRFGDVAFQQKKK
                     SYQQEYISYRELEKRVRKVQQNEKSYVQRIDMLHFQQEEIAAADLQVGEEEKLKEERE
                     KLSNYQKIADGLAAGYGALTNNEQNSVDGVGLAVSELQGIAHLDVEYEAIYENIQSAY
                     YLLQDAIGDMSRQIDLLELDESRLEEVTQRLELIRQLKRKYGESIESILAYYDEITEE
                     LASSDFSEGQLDKMKEELEQKELLLQQQAADLHEARKKIAKELEKSILHELKSLYMEN
                     TEFEVRFLKEENRQLNHDGFDQIEFYITTNPGEPLKPLVKVASGGELSRMLLALKTIF
                     SSEQGVTSIIFDEVDTGVSGRVAQAIADKISQISKYSQVLCITHLPQVAAVADYQYYI
                     VKAVIDGRTQTSVSELKTKEREEEIARMLAGSEITKLTLEHAKELLKLAKRT"
     gene            complement(22396..22512)
                     /locus_tag="IV334_000113"
     CDS             complement(22396..22512)
                     /locus_tag="IV334_000113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4044 domain-containing protein"
                     /protein_id="HAP8942218.1"
                     /translation="MREKKQTSTFAKVTKFVIWTMLILTIGSVVLTAVMSVM"
     gene            complement(22585..23541)
                     /locus_tag="IV334_000114"
     CDS             complement(22585..23541)
                     /locus_tag="IV334_000114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HAP8942219.1"
                     /translation="MIKYFTLDNEKLRNTGKETDQTIWYCVERPNQEEIHQLTHQFEI
                     PSDYITSILDDAENSRTEEFNQSKLTKPALLLLQYPFAKTSPSGYFQVDTYPLSIILT
                     PKKKLITITNHEPLFLKKVFSQTFPKSDISPNLNIFFQFIWQVTLSYNSYLATLQSQC
                     DTLEAKLQVSTENKQLYQIMDIQKSLVYFREATSANLDALKELAQNKLFQENHALKNH
                     LRDVLVETEQAMTTSRIQLKLVEQMNQTFSAIVSNNLNNIMKILTSLTIILTIPTIVG
                     SIYGMNIKLPIAERDDAFIWLVLLTLFICIVTTYYLKKGKFL"
     gene            23712..24080
                     /locus_tag="IV334_000115"
     CDS             23712..24080
                     /locus_tag="IV334_000115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3397 domain-containing protein"
                     /protein_id="HAP8942220.1"
                     /translation="MGTFTPLILFWYIYPVIVLFGCQFLVKLFSLNRRFKIKAPDLAI
                     PFLWGGMHALSRNTGTISILPFFFISVLLMGICIAVFQAYYYEEIIYPRYFKMTWRLV
                     FLLTIVLYAVLIIVNILSYL"
     gene            24250..24681
                     /gene="mraZ"
                     /locus_tag="IV334_000116"
     CDS             24250..24681
                     /gene="mraZ"
                     /locus_tag="IV334_000116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010754126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HAP8942221.1"
                     /translation="MLMGEFQHNIDAKGRLIVPSKLREELGEKFVLTRGLDGCLFGYP
                     MSEWENLEAKLNEMPLAKKDARTFVRFFYSAATECELDKQGRINIPSTLRNYAALTKG
                     CVVIGVSNRIEIWDETRWQDFSAAAEENFDEIAESMIDFGL"
     gene            24702..25661
                     /gene="rsmH"
                     /locus_tag="IV334_000117"
     CDS             24702..25661
                     /gene="rsmH"
                     /locus_tag="IV334_000117"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HAP8942222.1"
                     /translation="MTETFQHYTVMLKETVDGLNIKPDGVYVDCTLGGAGHSEYLLSQ
                     LSSKGHLYAFDQDQKAIDHAKLRLASYVEQGMVTFIKANFRELEERLSEHVDKVDGIL
                     YDLGVSSPQLDEAERGFSYHQDAPLDMRMDQSAPLSAYHVINEYSYHELVKIFFRYGE
                     EKFSKQIAREIERVRKTKPIQTTGELVEIIKQVIPAPARRKGGHPAKRIFQAVRIAVN
                     DELGAEEASLEQAIRLLKVNGRISVITFHSLEDRIVKSMFKEYSTVQDLPPGLPVIPD
                     EFQPELKVITRKPILPSEEELTENNRSRSAKLRIAEKIRMKEE"
     gene            25666..26067
                     /gene="ftsL"
                     /locus_tag="IV334_000118"
     CDS             25666..26067
                     /gene="ftsL"
                     /locus_tag="IV334_000118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HAP8942223.1"
                     /translation="MAELKKMQDYEYDLPELVDQPEQQMPDSSSNQEIILPQSPAKRL
                     KQISALEKIAVASVAIAIVALCVLTVMLRTNISGAEKEITSIQTEINDKKQEKTSLEQ
                     EKNELSRTERIKQIAEEKGLSINDDNLRKVK"
     gene            26068..28260
                     /locus_tag="IV334_000119"
     CDS             26068..28260
                     /locus_tag="IV334_000119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HAP8942224.1"
                     /translation="MSLKNKIRRFMEKKNLNPMNNRKKVGIILFATSIGLFFLFAVRF
                     SYIVIGGHVAGTSLAEKTKQLYQGSEVVKAKRGTIYDRNGVALAEDASSYSIKAILSK
                     TYTSGDKKLYVEEKNFDKIAEILHKNLSIDKKDALNILEDGAKKELYQVEFGSYGKNI
                     SQETKQNIEADMKKEGVAGLYFVDHQARMYPNGVFSSHFIGYAVPDKDENGLVGKLGL
                     ESAYNDILSGKDGKIIYQKDNFQNPLPGTVAEEEKAVDGQDIYTTLDSRLQSYLETLM
                     DQVNEEYQPEELTAVLMKAKTGEILAMGQRPTFNPETMEGLTGKDAIWRNFLVQDSYE
                     PGSTMKVFTTAAAIEEGEFNENETFQSGKIQVEDATINDHDFGEKGVLTMRQALSWSS
                     NVGMVILEQRLGGRWYNYLQKLGFGQSTHSGLDDEVNGALPTLNIVDRAMSAYGQAVG
                     VTNFQMMKAFTSIANNGTMIQPRYISKVVDPQTGEERTTQTEVLGQPFSKETTEKVRE
                     YMRDVVESENYGSAYGVYSVPGYNVSAKNGTAQIASDTGGYQTGDTAYLYSIVEMVPS
                     EDPDYVLYLTMKHPKTYDRMALAKIANPLMKRAMDFKETEEDSDTETKTEKVSVADYR
                     NLEADVAAADAQKSGLQPVVIGNGKKVQKQSTANGDQLISGEKLILYTGGDKLMPDVT
                     GWSKADIMKLGKILGIEVSFDGDGYCVKQELAPYEKITEDKLSFTLEE"
     gene            28286..29248
                     /locus_tag="IV334_000120"
     CDS             28286..29248
                     /locus_tag="IV334_000120"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HAP8942225.1"
                     /translation="MEWTQALIPIVSSCAMTIAAMPLFIGYFQMKKQGQAIREEGPKW
                     HNSKAGTPTMGGLVFLIGSILTGIWVGAWQKQLTPTLFILLFVLALYGVIGFLDDFIK
                     IFKKRNMGLNSKQKLIGQILGGIIFYLVYRSEGYPGVLNFFGLDLPLGIIYGIFALFW
                     LVGFSNAVNLTDGIDGLVAGLGSISFAAYGLIAWHQHQYDVLIICLSVLGGLLGFFVY
                     NRKPAKIFMGDVGSLALGGLLAAISIMLNQEWTLLLIGLIYVMETASVMLQVTSFKLT
                     GKRIFKMSPIHHHFEMCGWSEWKIDTVFWLISIVTSLITLWIVW"
     gene            29262..30623
                     /locus_tag="IV334_000121"
     CDS             29262..30623
                     /locus_tag="IV334_000121"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HAP8942226.1"
                     /translation="MKKISTYENKKVLVLGLAKSGVSAAKLLHELGALVTVNDGKPFD
                     ENPEAQELLSLGIKVITGSHPIELLDEEFSLMVKNPGIPYSHPLVAKAQEMGIPVITE
                     VELAYEVAECPIIGITGTNGKTTTTTMTGLLLNAGADQGIARLAGNIGYPASGVAQEA
                     KSEDKIVMELSSFQLMGITDFRPHIAVITNIYEAHIDYHGTRKEYVKAKWNLQKNMTE
                     KDYLILNWNQSELQELAQRTKARVLPFSTKEVLEDGVYADDYSIYYKKEKIMEISELG
                     VPGKHNVENALAAISVAKLYGISNEAIRETLHFFHGVPHRTQYVGEIQGRKFYNDSKA
                     TNILATKMALSGFETSKVVLLAGGLDRGNTFDELIPSLKGIKAMVVFGQTKEKLMDAG
                     KKAGIETIVTADSVEQAVPLALENSTDGDVVLLSPANASWDQYPNFETRGNRFMEAVN
                     RLK"
     gene            30649..31737
                     /gene="murG"
                     /locus_tag="IV334_000122"
     CDS             30649..31737
                     /gene="murG"
                     /locus_tag="IV334_000122"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HAP8942227.1"
                     /translation="MKILVTGGGTGGHIYPALAFVNYVKTQEPNAEFMYVGAKRGLEN
                     KIVPDTGMPFHTLEIQGFKRKISMHNIKTVRLFLKSIRQAKKILSDFQPDIVIGTGGY
                     VSGAVVYAAAKMGIPTVIHEQNSVPGVTNKFLSRYVDRIALSFKDAASFFPENKAVLV
                     GNPRAQEVADTKKSDILKTFDLDPEKKTVLIFGGSQGALKINQAVSVFLSEYIPSSYQ
                     VLYASGDRYYEEILNKINPEIQNISVQPYIKNMAEVMASSDLLVGRAGATSIAEFTAL
                     GLPAVLVPSPYVTNDHQTKNAMSLVHAGAAKMIADNELTGESLSQTVNEIMGDEELQK
                     QMCRASKEQGIPDASKRLYDLVKQIIKK"
     gene            31752..32972
                     /locus_tag="IV334_000123"
     CDS             31752..32972
                     /locus_tag="IV334_000123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsQ-type POTRA domain-containing protein"
                     /protein_id="HAP8942228.1"
                     /translation="MSKKKEPKKLSHPSSDENNLTPWQKANRKYLAEQEMKNQEPKKE
                     DITLDTPLSDSEEEKTVQEEQQEESQSEKKIEFFEEIKQKKNGGPYNGSFLNRLPNLK
                     SQRHKVLFRRLALIITVLGIPLIFLIYYVSPYSKLQAVAVSGNKTVNSQEIISDTKLS
                     LGENVWGQYFHRSTYIDRLKKAQPRIETANIHFKGMNEFDLDVTEYKEIALIAKNNQY
                     YPVIENGTVLDEKVANPTKNLPILEEFKDSAKIKELTKQYNQLSSELQKAISEIKYTP
                     RASNKNLIQLNMNDGNQVIVNINNLANQMKYYSQVAKDMDEKGVIDMEVGIFSHPYGS
                     TQETEESGQSEETTESEENADQTNESSEEAETQKAQTPGSSSTNGSSDSNDSSETSQI
                     EDNPSTTPSSLNEN"
     gene            33128..34453
                     /gene="ftsA"
                     /locus_tag="IV334_000124"
     CDS             33128..34453
                     /gene="ftsA"
                     /locus_tag="IV334_000124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HAP8942229.1"
                     /translation="MAKTGMYVGLDIGTTSVKVVVAEYIDSQMNIIGVGNAKSEGINR
                     GIIVDIDKTVQAIQRAVRQAEEKAGIQIKGVSVGLPANMLEVENCQGMIAVNGDSKEI
                     TDEDVRNVASAALVRSIPPERQIVSILPQDFTVDGFEGIKDPRGMIGVRLEMYGLLFT
                     GPKTIIHNIRKCVENAGLIVNEMVITPLALTESILSDGEKDFGTIVIDMGGGQTTTAV
                     MHDKQLKFTNLDQEGGEFVTKDISIVLNTSFNNAEALKINYGDAYPERTSPDEEFPVD
                     VIGQSEPVKVDERYLSEIISARMEQIFNKAKDALDQIEALELPGGVVLTGGAASLPGV
                     VDLAQEIFGVNVKLYVPNHMGLRNPVFTNVISIVDYSANLSEVYQLAKIAVTGETSAA
                     RQMVVEQETTNTYESYEAPEETIYDEPEPKESGENVKNKIKGFFTNIFD"
     gene            34476..35717
                     /gene="ftsZ"
                     /locus_tag="IV334_000125"
     CDS             34476..35717
                     /gene="ftsZ"
                     /locus_tag="IV334_000125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HAP8942230.1"
                     /translation="MEFSIDNNINDGAVIKVIGVGGGGGNAVNRMIEENVKGVEFITA
                     NTDVQALKNSKAETVIQLGPKYTRGLGAGSQPEVGQKAAEESEQSLREALDGADMIFI
                     TAGMGGGTGTGAAPIVAGIAKELGALTVGVVTRPFTFEGPKRGRFAAEGIARLKENVD
                     TLLIISNNRLLEVVDKKTPMLEAFREADNVLRQGVQGISDLITAPGYVNLDFADVKTV
                     MENQGTALMGIGVASGEDRVVEATKKAISSPLLETSIDGAEQVLLNITGGLDMTLFEA
                     QDASDIVANAATGDVNIILGTSINEEMGDEIRVTVIATGIDESKKERKSSRPARQAQM
                     QSPAQKTVLDMDQAKPTSSEEENSFGDWDIRREQNVRPRVDDSNFDNIEKKEFDTFNR
                     EETKSHGDDELNTPPFFRRKK"
     gene            35734..36411
                     /locus_tag="IV334_000126"
     CDS             35734..36411
                     /locus_tag="IV334_000126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HAP8942231.1"
                     /translation="MIADNLQKIKQEIQDSCALVHRDPKEVTLVAVTKSVESAQAEEL
                     VRAGVMNCAENRVDKLLEKKQDLFDFPEIKWHLIGNLQRRKVKLIVNEIDYFHALDSL
                     RLAEEIQKRLEKELSCFIEVNVSGEESKHGISPDELLPFVESLAACTKIKVVGLMTMA
                     PKEASDEQIHKVFGTLKELRDLVQAKGYAHAPCSELSMGMSQDFQIAIEEGATFIRVG
                     TALFKDE"
     gene            36425..37024
                     /locus_tag="IV334_000127"
     CDS             36425..37024
                     /locus_tag="IV334_000127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein SepF"
                     /protein_id="HAP8942232.1"
                     /translation="MPLLNRGAIANFFGLADEEEYEYQDYPETTKKAVQPTKKNTTQN
                     QSTHSVSAANTHEKQKTATSRPVTHESIQPAEKKKVIELHQSPTKTVAKERTASRTST
                     QPKANLAGKITVMEPRAYSEAMAIAKRIIAGEATLVNFHLVEEKQARRIVDFLTGTVY
                     ALDGDIKRVGDEIFLCTPTGMEIDSSTAQSFADANLFDL"
     gene            37039..37326
                     /locus_tag="IV334_000128"
     CDS             37039..37326
                     /locus_tag="IV334_000128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HAP8942233.1"
                     /translation="MYTIALLLHLLQRAAYFYSIILMVYALLSWFPGGYQSGLGRLLA
                     KICEPYISLFDRLPLRIGPLDFTILGAILILNLAVQGLAQIVIWITVHLVY"
     gene            37375..38157
                     /locus_tag="IV334_000129"
     CDS             37375..38157
                     /locus_tag="IV334_000129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HAP8942234.1"
                     /translation="MDANVYQHFRADERTFIDSVGDWIEQVQGQYAPYLTEFLDPRQS
                     YILETLIRQSSDLRFMFYGGYEQAERKRCLIFPDYYEPVEEDFETALYEIHYPSKFAS
                     LSHGKILGTLVGTGIKRSYFGDIISDGDRWQVFIANEVDHFVAAQVTKIGKVAVRLEP
                     IKYTEMIMPKDSWTQERGTVSSMRLDSVISEIYNISRQRSKQLIEAGKVKVNWTENTR
                     PDFVLELLDIISVRGYGRVQIQELEGKTKKEKYRVLFGVLRK"
     gene            38178..38885
                     /locus_tag="IV334_000130"
     CDS             38178..38885
                     /locus_tag="IV334_000130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DivIVA domain-containing protein"
                     /protein_id="HAP8942235.1"
                     /translation="MALTPLDIQNKTFPTKMRGYNQDEVDDFLDLVVRDYEELTQRNR
                     ELEKAVKHSEEKLEYFNELKDALNQSIIVAQDTADKVKTSASKESEVIVTSAQNKADE
                     LVANAEKRAHQLTTDAEEKARKILTDATEKARQLATETEDLKKKTRVFHQRISLMLES
                     QLEQVKSPEWDEILQPFSSYVTDSHEVIKEVLAKQLDNENDTDVNSDTGVIEAPMTAF
                     DTQAIDISIVPGENENE"
     gene            39147..41945
                     /gene="ileS"
                     /locus_tag="IV334_000131"
     CDS             39147..41945
                     /gene="ileS"
                     /locus_tag="IV334_000131"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HAP8942236.1"
                     /translation="MKMKETLQLGKTKFPMRGNLPNREGEWQKEWEENRIYQKRQELN
                     EGKPSFVLHDGPPYANGNIHLGHSLNKISKDIIIRSKSMSGYRAPYVPGWDTHGLPIE
                     QVLANKGVKRKEMSMAEYLEKCREYALSQVDKQRADFKRLGVAGDWEHPYVTLDPSYE
                     AAQVRVFGKMAEKGYIYKGLKPIYWSPSSESSLAEAEIEYKDVKSPSIFVAFNVKDGK
                     DILDEDTAFVIWTTTPWTLPANQGIAVNPKFTYVLVEADGRKFVVAKDLLETVQSEIG
                     WENVTVLKEFAGQEMEYMTASHPFYERESLVILGDHVTLDAGTGLVHTAPGHGEDDYI
                     AGSKYKLSVVSPVDSKGLFTEEAPGFEGIFYDKANPMITDLLKEKGALLKLDFFTHSY
                     PHDWRTKKPVIYRATPQWFASIDKFRQNILDEVEKVEWVIPWGKTRLYNMIRDRGDWV
                     ISRQRAWGVPLPIFYAENGEAIITPETIEHVAKLFAEHGSIIWFEKEAKELLPEGFTH
                     PGSPNGEFTKEKDIMDVWFDSGSSHEAVLRQRPELSFPADMYLEGSDQYRGWFNSSIT
                     TSVAINGVAPYKSVISQGFALDGEGRKMSKSLGNIIAPEKVIKQFGADILRLWVSSVD
                     YEADVRVSMDILSQVAEVYRKIRNTMRFLLANTEDFDPKKDTVSYNDLRSVDKYMTVR
                     LNQVIKEIREEGYDKYNFMHIYRTVMNFLTVDLSSFYLDFAKDVVYIEAEDDYQRRCM
                     QTVFYQTAVALTKLLTPIIPHTAEEIWSFLKEEEEYVQLSEFPGYEEFANQEELMDTW
                     TAFMDFRDNVLKALEEARNSKLIGKSLEAKLTVYPKQQVREMLKALDADIAQLLIVSP
                     DYFEVMAADEQVPDNAMVFDDVAITVEKADGETCDRCRQVRKDVGVDEKLPHLCGRCA
                     KIVEDFYPEAVAEGFEEK"
     gene            complement(41994..43520)
                     /locus_tag="IV334_000132"
     CDS             complement(41994..43520)
                     /locus_tag="IV334_000132"
                     /EC_number="1.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HAP8942237.1"
                     /translation="MSNEKNVLFTIFGGTGDLAQRKLYPSLFRLYRKGNLGEHFAVIG
                     TARRPWSDEHYREVVKETIRALNPSDEEATTFASHFYYQSHDVNDSTHYQTLKELSDR
                     LDGHYQLNGNRVYYLAMAPQFFGTIVSHLKSQNIMTKNGFNRLIIEKPFGSDYQSAFE
                     LNQQIREVFPEQDIFRIDHYLGKEMIQNISAIRFANNIFEAQWNNRYIDNIQITFGEN
                     LGVEDRGGYYDHSGALKDMVQNHILQVVALLAMEPPVAFSEKEIRTEKIKALKAIRIY
                     NEEEVLENFVRGQYAQGELDGVQFKGYREEDKVDAQSETETFVAGKFTIDNFRWSGVP
                     FYVRTGKRLTEKGTRINIVFKQVPVNVFKTSVDEPCDDTTLPPNVLTIYIQPTEGFSL
                     SLNGKEVGQGFETEPIKLEFRNSAEMVENSPEAYEKLLLDALNGDGTNFSHWEEVAQS
                     WHIVDVIRKAWDQTQPDFPNYKAGSMGPKAAFELLEKDGFEWIWQPDNWYRERGKLND
                     "
     gene            complement(43535..44182)
                     /locus_tag="IV334_000133"
     CDS             complement(43535..44182)
                     /locus_tag="IV334_000133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005881023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HAP8942238.1"
                     /translation="MTPNREDYLKIILELGGDTTKVNNKQIVSSLDVSAASVSEMISK
                     LVKEELVEHSPYQGVQLTASGLQKASSLIRKHRLWEVFLVEHLDYSWNEVHDDAEVLE
                     HVTSEHLADHLENYLNHPEHCPHGGSIPKKEEVVHEERRQTLESYPVGTKVRIARVLD
                     EKELLDYLVTIDLNINEEYEIVDIAAYEGPITIQNDRKKIPVSFKAASTIFVDKI"
     gene            complement(44366..44695)
                     /locus_tag="IV334_000134"
     CDS             complement(44366..44695)
                     /locus_tag="IV334_000134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4828 domain-containing protein"
                     /protein_id="HAP8942239.1"
                     /translation="MIYFLGSLITVLSSFIYLKKKKQAEKSEGLSKYQGTWFFSDSQQ
                     KQQLQITSDYTFLINGQSLGTSVVEIDEYRLVVRDQYGFYLIFSYPEQTLYDESADTS
                     YKLLSEK"
     gene            44872..45600
                     /gene="sfsA"
                     /locus_tag="IV334_000135"
     CDS             44872..45600
                     /gene="sfsA"
                     /locus_tag="IV334_000135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA nuclease SfsA"
                     /protein_id="HAP8942240.1"
                     /translation="MKYQQVEIAFFIDRPNRFIAHCMKKDGEIIIAHVKNTGRGKEVL
                     IPGAEVAIVFAPGPKRKTAYDLIAVKKKGDWFNIDSQLPNRLAIDGILDGTILLPGLT
                     GPFTVYKREVTYLHSKFDIYLETVVGQKAFVEVKGMTLENKAIGAFPDAPTLRGLKHI
                     GELTYAAQDGYAAYVLFIAQFEHLHLATIHEEMQPALADMVRHAQQSGVQILAYNCQV
                     SADEVTVKQAIPFDVDIPFEDPIQ"
     gene            45616..46629
                     /locus_tag="IV334_000136"
     CDS             45616..46629
                     /locus_tag="IV334_000136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HAP8942241.1"
                     /translation="MINLGIIGTNWITHQFVQAALSTKKYELTAVYSRHLEKAQEFGS
                     HYEGDIEFATDLKTFFNITHMDTIYIASPNSLHFSQAKQAILAGKNVIVEKPAFSTPK
                     EMDEIIHLANEHNVYFFEAARNIHESGFKKIADYLPLKDEIIGANFSYMKYSSRYDQV
                     LDGEEPNIFSTHFSGGALMDLGVYLVYAAVAWFGMPKEVHYFPRKISTGVDGLGWGIL
                     RYENFDVSIQPGKIGDSYLPSEIYFDSGTLVMNGVNAIEQAQFHDRAHKEIIDLAVTA
                     AENPMADEAEDFANVMAHPKDPAWGLLYEEWIELARNVNQVIYDLRKNGGIKFDADNE
                     EDF"
     gene            46629..47906
                     /locus_tag="IV334_000137"
     CDS             46629..47906
                     /locus_tag="IV334_000137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HAP8942242.1"
                     /translation="MNEFVQVFPENWQKKYEDKGFISPSLIQRAVFQPLSNKQSIVAV
                     SPTGSGKTLAYLWPLLLNVEPGEASALVIFASSQELAIQVADVAREWGKDKELKVQSL
                     VGGANVKRQIEGLKEKPEILIGTPGRILELMKAKKIKAHQVKTMVFDEADQLFDAGNS
                     QIIDQILHQAPTEYQLAFFSATADRSLESIEKITGKTFETIDVTAEDDSRKGLRHYFL
                     RVPIRKKDDYLRRLTHIEDFHGLIFFNQLTELGVMEEKLLYRGVPVGSLASDQNKLLR
                     KAALEQFKKKMVRALLTTDVAARGLDITGLPYVVNAEVPLSEEAYLHRSGRTGRMGNE
                     GIVITLVQDNNLRDLKRLLRPSNITLEEIFLYGGKLQTEQPTKEDAAPAVNKKYAKTF
                     SGKEQNPEYNKEKIRKKVKNKSKKERNKGARRK"
     gene            47969..50671
                     /locus_tag="IV334_000138"
     CDS             47969..50671
                     /locus_tag="IV334_000138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAP8942243.1"
                     /translation="MKERQEEILQLLTENQQGLTASDVAERLTIDRSNASRYLSELYK
                     AHHIVKTAGRPVVYSLPTEKSKSDEVHVDSSTQVTFETLVGENDSLKVSIQQAKAAIL
                     YPPRGLHTIIFGETGTGKSMFAECMYHFAIDSEMLSADAPFVSFNCADYAQNPQLLFG
                     HIFGIKKGAYTGAAQDSPGLIAKADGGILFLDEIHRLPPEGQEMLFTFIDKGIYRPLG
                     ESAQVHEASVQIIGATTESSESFLSTFNRRIPMAITLPNLASRSLDERYEIISLFIKQ
                     EANRLNQRIDIEKEAILAFMLYDAEGNIGQVKRDLKLVCAKSFLHYRTHHEKKLIIRK
                     EELSLQVQKGLLKIKEIPERLDRFMDSKSQYLTFEPGFADVVWSQDPERNMQVYNDIE
                     EKVTSLSETGVENIDLETLISKDVDAYFQTYVKELTKSSIPKDLLPDEIWRLTNQLYD
                     VAEKRLDRIYNEKARFAFALHLQSTLDRVREGHMIVHPDLNNVRKNFKREFQVAIDLS
                     TIIEEEEQVEIPFDEIGFISMFLSINLGESEPVKENKVDVLILMHGRQTASSMLEMAQ
                     ELLGTKVGKAFNMPLVMEVQTMYEEVLQYVKNYQEHLTNGLLLLTDMGSLNTFSDLIY
                     EETGIRTKAITMTSTMIVIEAIRMASVGRSLEDIYQNIQLSFESIVRDQFKAEKSETL
                     QRKKAVIVTCFTGEGVAAKLYQRIMPVINQNKVELIQMQFLERESFKKHIDALLEEYE
                     IKAIAGTVDVDYQNIPFFSAYDVFDDEKLNILKRIADDEIPTAKIVDSLAGSLSDVFS
                     IDELIRSLQKTVQQIQTDLSIIVEPGVDTGIVIHLAFMLDGLIKQESHRQFEKLSIFS
                     KIYRLEMDIVRANLMALERKYQVKIPEDDIAFLTQMFLENKIKK"
     gene            50823..51140
                     /locus_tag="IV334_000139"
     CDS             50823..51140
                     /locus_tag="IV334_000139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAP8942244.1"
                     /translation="MAKKTIMLVCSAGMSTSLLVTKMQKAAEEKGMEADIFAVSASDA
                     DNNLESKNVDVLLLGPQVRFMKAQFEQKLAPKGIPLDVINMQDYGMMNGEKVLAQAEN
                     LMK"
     gene            51170..51490
                     /locus_tag="IV334_000140"
     CDS             51170..51490
                     /locus_tag="IV334_000140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HAP8942245.1"
                     /translation="MEDQQNLEAIMGLIMFGGNAKSDAMEAIAAAKIGDFELADRKIA
                     DAEESLIQAHHAQTDMLTQEAQGNHMQVTLLTVHSQDHLMTSIAFTDLAKEIIELYRR
                     VYSE"
     gene            51598..53058
                     /locus_tag="IV334_000141"
     CDS             51598..53058
                     /locus_tag="IV334_000141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAP8942246.1"
                     /translation="MNGLSSWMEKYILPVAAKIGAQKHLVALRDAFIGTLPATMAGSV
                     AVMINAIIRDLPQQFNSTYADSLAADKGIFAVLNVIIGINGFVWNGTLAIAGLIFAFS
                     WGYNLARAYGVNDLAGGIVGLATLIQGISFGPALSAALDVNVPSNVADAINSSEIGAT
                     IADGTLSVGLWGWLKLDHLNGNAYFTVMIMGAISVIIFCKLMLANITIKMPDSVPPAV
                     SKAFAAILPATIALYVIAIINYIVGRVADGQLIIDLVQKYIAEPFLGLSQGLGAVLIV
                     TVFVQVFWFFGIHGPNVLAPVLEGIWGQAQLINIDIFQKGYNGKTGTPAVLDAIEDGK
                     AYMWVRGSFDAFAWFGGSGGTIVLLIAILIFSKRADYLTVAKLSIGPGIFNINEPVMF
                     GLPIVLNAIMFIPFLIAPVVATAIGWTATYLGLVAPVSQQVTWVVPPFLLSFLATGAD
                     WRAPIVTLVCMVVTFLIWAPFVISANKMDPAAGEQQ"
     gene            53126..53347
                     /locus_tag="IV334_000142"
     CDS             53126..53347
                     /locus_tag="IV334_000142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8942247.1"
                     /translation="MITIASAMQQDEIKELLEGYNEEGMTFTFKEKQGIKLFFETSAE
                     DPEVAAKKAKELIKAQPWGTVLYFQSTAV"
     gene            53378..53560
                     /locus_tag="IV334_000143"
     CDS             53378..53560
                     /locus_tag="IV334_000143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3188 domain-containing protein"
                     /protein_id="HAP8942248.1"
                     /translation="MIKNGLFLCSIGSLIVLYSLNPHSQRYDLYSMVTGLFLTGLGIY
                     FFMKGKKREEAKEKNN"
     gene            53560..53973
                     /locus_tag="IV334_000144"
     CDS             53560..53973
                     /locus_tag="IV334_000144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3284 domain-containing protein"
                     /protein_id="HAP8942249.1"
                     /translation="MEITKKLNIPASFFYDKVMDSVLFDVRKATGKSVTRKQLKNLEY
                     VKQFSQNNRARIKIEEVIENQSYKFRTSTTKNEFVVHYQIKPLDDSTCEVHYTEKMES
                     YGMLQKLNDMLLGTILMYFKRRRFKKMLDMIEATY"
     gene            54096..55277
                     /locus_tag="IV334_000145"
     CDS             54096..55277
                     /locus_tag="IV334_000145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP8942250.1"
                     /translation="MTNKVRELGVSSIEEMFDLATYAFNGADTPERRERFRTLAENSW
                     NYGFFDEEDRLTSQVMATPFPVNFYGQEYLMAGIGYVASYPEARGQGGINQIMEKILE
                     DCRDRKVSLSYLAPFSYPFYRRYGYEQSFDKISYQLNSRDVPFIKKKSGKIKRMDFED
                     AKAAIRRIYEQMPENQRAGLKREDWWYTYKFKQKGNYQFALHFDDNEEATGYIVYQLA
                     TSSFIIAEWGFLDHDAFCSLVRFVSSHNGAFETFEYSCGYGGNDQNYLLENPFASVMI
                     TPYMMARIVDISLFLENYPFKKRQAAFALQIEEDHYAKWNEGIVEVTIENGKNHLSKV
                     EQTKLPIVRGSIQHITQLLLGYRKVEELVFQERIQVTKEYEETLSSILPQQQPILNDY
                     F"
     gene            55666..55739
                     /locus_tag="IV334_000146"
     tRNA            55666..55739
                     /locus_tag="IV334_000146"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:55700..55702,aa:Arg,seq:cct)
     gene            complement(55776..55976)
                     /locus_tag="IV334_000147"
     CDS             complement(55776..55976)
                     /locus_tag="IV334_000147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HAP8942251.1"
                     /translation="MKINYTVDLVSFSLVINFSLFSLRLFIDGLRHTHASILLYQGVN
                     ILSVSKRLGHSSLETTMSTYTK"
     gene            complement(56281..57513)
                     /locus_tag="IV334_000148"
     CDS             complement(56281..57513)
                     /locus_tag="IV334_000148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HAP8942252.1"
                     /translation="MLSDFNELLKKYARLIAETGVATEKGHTVVLQISVEQAPLARLI
                     TQEAYKLGAAEVIVQWTDDQIQREFLLHAATDRIENVPQSKIDQADEWLEKGASRISV
                     VSADPDAFAGVDSHRVASYQSANGKALMNLRKATQANKVSWTVVAAAGKQWAAKVFPE
                     LPEEKQVEALWDQIFKTTRVYEEDPVLAWKKHDEKLAKKAEELNREQFSALHYTAPGT
                     DIIIGLPKNHLWEGAGSYNARGEKFMANMPTEEVFTAPDSHRVDGYISSTKPLSYAGT
                     IISGMKFTFKDGKVVDFSAEQGEDVLAKLLDTDEGARRLGEVALVPDPSPISQSGIIF
                     FNTLFDENASNHLALGSAYAFSVKGGTEMSDEELAEAGLNRSQTHVDFMVGSDKMDID
                     GIREDGSTVPIFRNGDWA"
     gene            57767..58336
                     /locus_tag="IV334_000149"
     CDS             57767..58336
                     /locus_tag="IV334_000149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAP8942253.1"
                     /translation="MARKKTITREQILAAAYEVAATDGFTRFTARNIANKMNCSTQPI
                     YLEFKNMDDLRQALFEQIYDHLQYEVFTVEHTGNTIIDLAFNYIHFARREKKLYRSLY
                     LEEYGGGKEMQNFSYTYFVNTVKRDPAYVNLSEEQIESLYNGTWIIATGIAALMSSSI
                     IHPTDEQIEKMIQDSIDAILKFKEPIEID"
     gene            complement(58514..58954)
                     /locus_tag="IV334_000150"
     CDS             complement(58514..58954)
                     /locus_tag="IV334_000150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HAP8942254.1"
                     /translation="MALAKIVYASMTGNTEEIADIVAEAFENLDIEVEINECTQVDAD
                     EFEEADICVVATYTYDDGDLPDEIVDFYEDLQELDLLGKIYGVCGSGDTFYDEFCKSV
                     DDFDAAFAKTGASKGAENVKVDLNAEEEDIENLEAFAKELVAKI"
     gene            59112..59876
                     /gene="map"
                     /locus_tag="IV334_000151"
     CDS             59112..59876
                     /gene="map"
                     /locus_tag="IV334_000151"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008377375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HAP8942255.1"
                     /translation="MITLKSAREIEMMAESGALLADVHKNLRDFIKPGITSWDIEVFV
                     RNYIESHGGIAAQIGFEGYEYATCISINDEICHGFPRKKPLKNGDLVKVDMCIDLKGG
                     ISDSCWAYVVGESTPEIDRLMEVTKKALYIGIEQAQVGNRIGDIGHAIQTYVEGENLA
                     IVRDFIGHGVGPTIHEEPAVPHYGEAGKGLRLKEGMVITIEPMVNTGTWKMKMDPNGW
                     TAYTRDGGLSCQYEHTLAITKDGPRILTSQGEEGTY"
     gene            59908..60831
                     /locus_tag="IV334_000152"
     CDS             59908..60831
                     /locus_tag="IV334_000152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HAP8942256.1"
                     /translation="MKVWNKIKQNENLMRFIETTQKRITDSEMSTTSVVVAYYLLLSL
                     FPLIIAFGNILPFLHIDQETVLTYLREVIPETVYQFIGPAIKDLLTQSSGGLLSVSAL
                     AALWSASQSINALQIAMNKAYGVENRKNFLIVRFFSLIVIVLFMIAISGVTIVLGLGQ
                     LILDAIQPIVQIPVNFIDQFQTLKWPITLVALFVIMFLIYLIVPNAQLKLKTIIPGTI
                     FATVGWMLLSQVFSIYARYFATRISGYQIIGSFIVLMLWLNFAATIIILGGIINAVVQ
                     EYVTGHQIKKRRDPSRKWFNKLKTKFSHKKK"
     gene            60907..62046
                     /locus_tag="IV334_000153"
     CDS             60907..62046
                     /locus_tag="IV334_000153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl/decaprenyl-phosphate
                     alpha-N-acetylglucosaminyl 1-phosphate transferase"
                     /protein_id="HAP8942257.1"
                     /translation="MLVSFSMVIRFFLTILLSLILTPVFKIISVQTGMVDKPNERRIN
                     KVPMPSAGGLPIFISFVISTLFLFRKIIPQSYIVPILIASLVIIVTGVLDDKYELTPK
                     QKSIGILIASLIIYFVADIRIDSFTLPFIGYIQLGWLSFPVTIFWIFGITNAVNLIDG
                     LDGLAAGISLIGLITIGIIGYFFLHASTVYIPVVIFCLVASIIGFFPYNFYPAKIYLG
                     DTGALFLGFMMAVLSLQGLKNVTFVSSISLLIVMGVPVTDTFFAIIRRKANRVSFSTA
                     DKKHLHHRLISLGFTHKGAVLTIYAMALMFSFTAMVMNYTGRIGTIVLIIAMLFAAIL
                     LFELIGLINEKHQFILKTLRFLGNKEYRTQVKQKYLKKWNRHIDE"
     gene            62039..62839
                     /locus_tag="IV334_000154"
     CDS             62039..62839
                     /locus_tag="IV334_000154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HAP8942258.1"
                     /translation="MNNKLNQPILISIVTANSDKIFRTLDNFISTVKDETAVKIRIFD
                     NNSLPEYKKRLTEYRKYPYIDIYFHDENSGFGYGHNHNLIASDSRYGIICNPDILVDE
                     ETVTALVLLLKEHPECAMLAPKILNEDGTTQHLIREQLAVFDYVLRFVPFKWVKKKFD
                     KRLAVFECRGLPTDANSYVRMISGSFMVADIDKFKEIKGFDERFFMYFEDNDLCLSFE
                     KAGYKLLYTPFYAVIHFYGKGAHRNAKLFRVFLTSMMKFFNKWGWRFF"
     gene            62839..63666
                     /locus_tag="IV334_000155"
     CDS             62839..63666
                     /locus_tag="IV334_000155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HAP8942259.1"
                     /translation="MKPEILVVVVLYQQLFSQSPAYGPLSKALSEKQIQLIIYDNSPQ
                     RQQNELFEKENIVYHHDPQNPGLAVAYNFALSQADEGTRCLVTLDQDTRLVDDYFELL
                     RKVTFTDECVAAVPMIFSGSRQVSPLYSDRYINRTAQAVEVEGPTAERIMAINSGVAW
                     SVKFLKKIGGFNPAFSLDFLDHWLFWKVDQLEKTVEILPVRMEHDLSVLDYEKVSSNR
                     YRSILQAENRFYHEYDQRHLSSHRRQLLLRTAKQFLTVKNRHIWRMTLRSYVKNWKV"
     gene            63644..64378
                     /locus_tag="IV334_000156"
     CDS             63644..64378
                     /locus_tag="IV334_000156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HAP8942260.1"
                     /translation="MLRIGRCEFVRSVCIATFNGGRYLREQLDSILSQLSAEDEVVIS
                     DDGSVDDTWDILTEYAEKDKRIQLFSGPRQGLIANFGYAIQQTKGDLIFLADQDDVWL
                     ETKVKKITDYFEQNPDQLVVVSDLIIVDSELQEIHDSYFSFRNSKEGWLNNVIRSHYI
                     GAGMAFRSSLKPDILPIPSEVPMHDMWIGLLGGKKTGFLREPLTLYRRHDLNASEIET
                     HSKLRQKISWRVHLITALIKRKWFKR"
     gene            64478..65344
                     /gene="rfbA"
                     /locus_tag="IV334_000157"
     CDS             64478..65344
                     /gene="rfbA"
                     /locus_tag="IV334_000157"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HAP8942261.1"
                     /translation="MKGIILAGGSGTRLYPLTKATSKQLMPIYDKPMIYYPMSTLMLA
                     GIKEILIISTPQDTPRFKELFGNGNDLGLQIEYAVQESPDGLAQAFIIGEEFIGDDSV
                     CLVLGDNIYYGGGLSKMLQRAASKDTGATVFGYHVNDPERFGVVEFDENMQALSIEEK
                     PAQPKSNYAVTGLYFYDNEVISIAKGIKPSERGELEITDVNKAYLDKGKLSVELMGRG
                     FAWLDTGTHESLLEASTFIETIEKRQNLKVACLEEIAYRMGYIDKEQLLALAQPLKKN
                     QYGQYLLNLAAE"
     gene            65358..65930
                     /gene="rfbC"
                     /locus_tag="IV334_000158"
     CDS             65358..65930
                     /gene="rfbC"
                     /locus_tag="IV334_000158"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010771301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HAP8942262.1"
                     /translation="MKVTDTKLTDVKIVEMDVFGDHRGFFTESYSKAKFAEHGLKYDF
                     VQDNHSLSAEAGVLRGLHFQKGEAAQTKLIRVVTGAVLDVVVDIRKGSPTYGQWDGFI
                     LSEHNHRQLLVPKGYAHGFVTLTPNVNFLYKCDNYYNAEADGGIAFDDPDLAIDWPID
                     PSRAIMSEKDHRHPTLKEFEAENPFVYGEI"
     gene            65952..66980
                     /gene="rfbB"
                     /locus_tag="IV334_000159"
     CDS             65952..66980
                     /gene="rfbB"
                     /locus_tag="IV334_000159"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HAP8942263.1"
                     /translation="MKNIIVTGGAGFIGSNFVHYVVNNHPEVHVTVLDKLTYAGNKEN
                     LAGLPSDRVELVVGDVADAELVDRLVKEADAVVHYAAESHNDNSLKDPFPFVQTNIIG
                     TYTLLEACRKYDVRYHHVSTDEVYGDLPLREDLPGHGEGEGEKFTAETPYNPSSPYSS
                     TKAGSDLLVKAWVRSFGLRATISNCSNNYGPYQHIEKFIPRQVTSILSGIRPKLYGEG
                     KNVRDWIHTNDHSSAVWLILTKGRIGETYLIGADGEEDNKTVMELILEMMGQPKDAYD
                     HVNDRAGHDLRYAIDSTKLREELGWQPEFTNFRDGLADTIKWYEEHEDWWKKEKEAVE
                     AAYAKNGQ"
     gene            67078..67929
                     /gene="rfbD"
                     /locus_tag="IV334_000160"
     CDS             67078..67929
                     /gene="rfbD"
                     /locus_tag="IV334_000160"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HAP8942264.1"
                     /translation="MFLLTGGNGQLGTELRHLLDEKNLPYVSTDAKDLDITDAEKTMA
                     YITELKPDVIFHCAAYTAVDKAEEEAKELDEKINVDGTRNVALAAKAVNAQFVYISTD
                     YVFDGKNKNVEYEVDDQTNPLNEYGRTKLLGEKAVEEILEDYYIIRTSWVFGIYGHNF
                     VFTMQRLAETRDKLTVVNDQFGRPTWTRTLAEFMVYVIEEKAPFGIYHLSNENSCSWY
                     EFAKEILKDTDVEVAPVTSEEFPQKATRPQYSVMSLKKTEALGFVIPTWQEALAQMLE
                     AAKKMNK"
     gene            67964..69997
                     /locus_tag="IV334_000161"
     CDS             67964..69997
                     /locus_tag="IV334_000161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8942265.1"
                     /translation="MEKQTRSARRKEKPKANQADSNLVLDLFLKLRYLIGVLVIVVIV
                     TFNLNGSSLGVWDKYVSQRDDGKKTDVIFGQNREVRSDEWLVQTPFYLSQAEEDFSLV
                     NEDYSLNGQNMIIAYNSPVKDITVIGKPFNWGFFFLGRDRGLSFYWAMKLVVMVLLGF
                     EVLMILTKRKKALSLIGAFALTFSPAVQWWFMQHVGDLIFFTLGLMIAFYHYFYNHDK
                     KWLRTLMMLLVVIFGIGFILVIYPAHQVMLAYLLIFYFVGLLIYYSKKVSWDLLDVVL
                     IGGAVLFIGGVMVHFWLTSKDALLASLNTLYPGKRVSTGGKWTIGEFFSFLTNWKIPF
                     EDINYSNNSEVALFYHFFPTVFLASPFVLFGKKDSEQKLLGRILMIFCLFSIFWITVG
                     LPKWLAEITLLSYVPPLRAILTFSFAACLLSIWFIAYIWREDVIPIWYKFILLAVNAW
                     SYFYAITHSKMDNYFSDFETIAVAVLGTVILAFALFGWRRLFYLAFVALVIVSGFFVN
                     PVVEGTGAIFEKTLAKEIQKIDQQDPGKVWLSEDELYNFTPTLGVKSFNTVRFYPDIA
                     AWKKIDPKEEYEKYYNRYAHTRAFIAKEDTKFILDRPDMFSVTLNFEDAEKLGIDYVI
                     SKRPLTDYNQLYEAQFTQLYGPDKDGYMIFKVEYPDYPDVPQTQTQENQLVVQ"
     gene            70041..71321
                     /locus_tag="IV334_000162"
     CDS             70041..71321
                     /locus_tag="IV334_000162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EpaQ family protein"
                     /protein_id="HAP8942266.1"
                     /translation="MKIIQAIVQKFVNKMSVGFLLLLLTLVFFVWTAGVGFPATLFIY
                     NNSSLLIAFSVAILLILNVYKAKGVDFLYLGVALALFIFFSYFRELRNESLFGDLLFP
                     LVILFCLVIKWIEWTKWDRGIYLIVFTVFLGITLYRVFTEIQVPEGQSIWAPGNSLSD
                     IWINTNTIGSSLMTLALLISGFASSFERWYVRIIGGIAVVAAFLGIWVCQSRGALVAL
                     IVFAILDLLPKSLMRVIRAPFIAYTVTILLALPISYLAAVSEKVNLFTDREDIWHKFY
                     QTLGEKSEQVLLGMKTFIFQRGNQFLGNHNSYNSILNIYGLIGFGIAALLLILFIGRL
                     TLKADLSNGQMTFIWAFFAVMMQSFMEDTLTSYPWVPIIYILLAMAVHRYDEPEVAEE
                     FYVMEEAYDDMTYDDTSYEEQPDSAHSSRIQRYH"
     gene            71531..72337
                     /locus_tag="IV334_000163"
     CDS             71531..72337
                     /locus_tag="IV334_000163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP8942267.1"
                     /translation="MFKDLYLFMKDIYQNRKLLLQFSINDFKSRYAGSFLGILWAFIN
                     PLFTVLIYWLVFGVGLKAQMIDAKYPFIVYLVTGMIPWFFFSDAFASTTVVFREYTYL
                     VKKVVFNIRILPTTKILSNLYTHLFFILIGIAVALINGIYPSAMSLQLIYYLFCMCAF
                     LTGLTWLTASIQPFLPDIMQLITIVLQLIMWTLPILWSPNQFDSGLVSILKLNPLYYV
                     VQGYRESFLSEAWFWEHGTYTLYFWVFTLVMFVIGSAVFRRLKPHFSDVL"
     gene            72349..73569
                     /locus_tag="IV334_000164"
     CDS             72349..73569
                     /locus_tag="IV334_000164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP8942268.1"
                     /translation="MTEYAIRLKDITKSYNMYAKPSDRFREALNPFKKSYHDVFYALK
                     NVNVEIKKGEMIGFVGENGSGKSTMLKIITGVLTPTSGTMEIEGKISALLELGSGFNP
                     EYSGYENIYLNGMVLGFSREEVDEMVDDIIEFADIGDHIYQPVKTYSSGMFVRLAFAV
                     AINVNPDILIVDEALAVGDLEFQLKCMEKFTEIKNSGKTILFVSHDISSIRRFCDRSY
                     WLKNGEVVEYGDTMEVTTNYENFLKKKSVKTVDRNKNQVERFAAPDIVDVTKAELLDK
                     DKNPVDMLEQGEKLYVKVTYEVKDDTIKNPVLGIALRTVDNFYVCGLNTLLDGIKIPW
                     KKGENIFYLEYEKLGLLAGEYYFDVAVFEENATVPLVYKTKYMNLFVSGSYIGEGIVV
                     LDHKWEEGTHSNEI"
     gene            73559..75145
                     /locus_tag="IV334_000165"
     CDS             73559..75145
                     /locus_tag="IV334_000165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAP8942269.1"
                     /translation="MKYDFEMDLDEQSSVGKIAAQIKPGSKVLEFGPGNGRLTKHLIG
                     AKQCEVSIVELDKELFDFVSEFAQDGFYGDIESFEWANYYAGQTFDYVLFADVLEHLV
                     DPGKTLKKVREFLNEEGEILITFPNLAHNSVMIDLFNNQLPWASYGLLDETHNSFYTH
                     DGFQKVFEKAGLFINIEDYLYLAVGDTELKSTYEELPEAVRYDFKMRPFGEVYQYFFS
                     LMKHPVAQSSIAEPQNSNYVKVLEVTQQTKQDETIQQIPFNNFTGENETLSFPVSETT
                     ERMVFRFAQQPSFIEFSAEAAGEKLTFIDSNAVVKTVNDCYLFDGKELPEFVLTDISG
                     KEVTIHCHYRFIGELTPTMKELLETIRPMAEVTKQLSEKNDELERENHHLLEENTRLD
                     HTLKTTTNRYCTLLESDEWTIKHRLGRRKKETSKKIQEKELSICIDEKIWDAETKILK
                     IIGWGIANSDRQPLSYKLSADQSPFFEAIPVSREEVNQAEQLAKGTEAGFELRILCEQ
                     ERSFLVEAVAQNGQSWIIEM"
     gene            75184..77322
                     /locus_tag="IV334_000166"
     CDS             75184..77322
                     /locus_tag="IV334_000166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HAP8942270.1"
                     /translation="MQKKEIAVFIDQITRERATGDLLIVGWAIDEVTKEIPTIKVEKE
                     NVIAEATHVVRLDINHLYNLDVKTQSGFKVRLSGKMRGKAILDFQTARHQNGIAVKLN
                     GKYPYDDGIESSWERKKRLLKKGINYARTHGVKKAIRRVKLELKPGSIDYAEWISRHE
                     KPDLKEQRKEAQGFAYRPLISILMPVYNVEIKWLEKCIDSVLDQTYDHWELCISDDAS
                     TDPAIRKCLESYQAKDDRIKVVFRKENGHISLATNSALEIAEGEFIALLDNDDELPPF
                     ALYEVAKVLNVHPELDVIYSDEDKIDADGNRFDPHFKADWSPDTLMGNNYISHLGVYR
                     TSIVKELGGFRKGYEGSQDYDLVLRVTEQIPADHIYHIDRVLYHWRTIPGSTASNGEA
                     KSYIYDSGVKALTDALSRRNIKGSVRPGRISGFYEIAYDVLQEDLVSVIIPTKNGYED
                     LKTCVDSIIEKTSYSNYEIIIADNGSTDPKMQELFAEYKHQLKDRFIVELIDIPFNYS
                     RINNLAAEKANGKYFLFLNNDTEVIEPDWMTVMVSYAQFDRIGCVGAKLFYPDDTTQH
                     AGVLLGIGGVAGHALNNYDRTHCGYFGRLVIDVNYLAVTAACMMVKAADFNAVNGFDE
                     TLEVAFNDVDLCLKVYELGRYNVYAHQAELYHFESKSRGYEDTPEKQRRFAGEIKKMQ
                     DKWPAYIAHDPFYNDNLTKDGIGDFSLRPD"
     gene            complement(77690..78673)
                     /locus_tag="IV334_000167"
     CDS             complement(77690..78673)
                     /locus_tag="IV334_000167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HAP8942271.1"
                     /translation="MNKQELHDFIGFRQPNICQIVAYKDGVEVYTDEWNGYKKDDTCH
                     IMSATKSIVSLLIGIALEQGLIKSIDDKVLDYFPEYKVKRGEKTIYAITIKHLLTMKA
                     PYKCKGDPWTKVCSSEDWTKTSLDLLGGRKGITGEFNYQTVCLHILTGLLYQTSKLTT
                     VDFANKYLFDPIGIPNHVNYYAETAEEHKQFTMDKSPKTNVWFCDPLNIGTAGYGLCL
                     SASDLAKIGVLCLNKGMYKGKQIVPPMWINEMFKPRTSALEDLQNMRYGYLWWVLDSE
                     NEIYSAIGNSGNVLYIDAKNNLVISITSYFKPMIIDRVAFVEEYLKPFILK"
     gene            79051..80442
                     /locus_tag="IV334_000168"
     CDS             79051..80442
                     /locus_tag="IV334_000168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HAP8942272.1"
                     /translation="MNKNGEWSAARRIFIILMDVIVYNAAIYSSFLLKFRGEIPLRNL
                     ETFQHSAIIISVIFIALNILLGAYVFYNRMISDIVFVTVIGQVLMTLGIMVVTFAGRW
                     FAFPRLVILLSFVLGTLFLSIYRILVYKLYLRVSRDKKVVIIGLEKDVAPAIQNFGSK
                     KNNKHKVEAVILDNYYANIKNMIKDIDIVYLASHIEETEKIKIYQLVTKNEKKLFLNT
                     TFENLTLINPNIMNFEDESIIEASGFRIQPEYELFKRLFDIIVSLVLLVIASPFMLFT
                     AILVKVTSPGPIIYKQVRITKNQKEFSIYKFRSMTATAEAKSGPVLAKSNDARVTPVG
                     KFIRAVRFDELPQIFNVLKGDMSIVGPRPERSFFVDQFNAENPYYYLRHNVRAGITGY
                     AQVYGKYVSDYNSKLRFDLLYIKKYSLMLDLKILLQTIKILFDKMSSQGLEEDENVIS
                     LAEIAFPNEKIFR"
     gene            80458..81498
                     /locus_tag="IV334_000169"
     CDS             80458..81498
                     /locus_tag="IV334_000169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HAP8942273.1"
                     /translation="MNNPKFSIIVPVYNAEATIGRTIDILKELDYEEYEVVLVNDGST
                     DQTQEIIQELIENDERFELITTTNQGPGLARNEGIRQSKGEYLLFFDADDTPVKTILS
                     DYDHLLEKSPKADLVVSSFVFRTMDEGRIVSEKENLVTECHYLTHEEFIKDMYQLMNE
                     QLMYVVWNKCYRSDILKNNQILFKTYNSCEDRIFNLHYYEYCHQVIMNPKVEYIYEFE
                     GGKGITNQYRPNKFETFKEFYSLANTVTDDINKDGMAALLLKGTTSVIFSIYSTSART
                     SKEKKDEAKQILSDPVIREAKQIAKTDSSAKKITKLLYNMPAPLFLSAVKVGSFVEVK
                     MPGLMAALKRVY"
     gene            81517..>82558
                     /locus_tag="IV334_000170"
     CDS             81517..>82558
                     /locus_tag="IV334_000170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HAP8942274.1"
                     /translation="MKRILHFQGRMGLGGAESFMMNLYRKIDRTNYQFDFLIYEDYAD
                     VQDYHSEIERLGGRIFVVPNPKKNIFKYLIEVNKLLKKESFSIVHNQVYFGGGINLWL
                     AKKNGIRQRIAHSHATEDGKSQNIVMNVLRKFLTKLLLQNATDYLAVSQEAGESLFQN
                     HPFEIVHNGIDLELYSKNSEAKVNKRKELDISMSTFVVGNIGRLEKQKNQSYLLEIFL
                     EILKMNSDSYLILVGEGSLRNKLKAKAVELDIEKRVKFLGERNDIPELLSVMDVFVMP
                     SLYEGLPMVGLEAQAAKLKLVLADTISKDTKLTNNVDFVSLDETPQEWAKKVIKEPYA
                     NKITQELKSYDVS"
BASE COUNT        26985 a        13915 c        17491 g        24167 t
ORIGIN      
        1 ttccccgaat ttttggtttg actccttata ttattgagga caaagaaatg agtccatatt
       61 atgcacaagg aaatctgata tatgtacgac cgaaaaatac aaatgagatt ttagtcggag
      121 atattattac atattatgaa aattcaggaa aaaaaatctc gacgcgtcgc gtaatagccg
      181 ttgaaaacaa ccacgaagat ttttatacaa aagctgatac aaagacttat attgagcctg
      241 gagtagtaaa aaagagaaat attatcggta gtcccatctt tcagattcca tatattggac
      301 tagtcctttc aaaaacggca tttgcatgga ttcaggggtt attctttacg attgcttttt
      361 gcctaacagg aatcacagca tggaatacct ttgtagagtt gaaaaagaag cggagacgaa
      421 cacagaaatt tttgtaagaa tgttaaaaac ataaatgtat tccagagatc caacgactct
      481 tctgaacatg aaacacgaga aaacactcat gcagcttgta gatttagtca gttgttcgta
      541 taatgtgtga agaggaggag tagtatgcgc aatgaaatga tgcatcgtcc tgtagtcaaa
      601 gaagaactat tggattttat gcgtacccag caaaaaaaat taccgggtga gctaggcaaa
      661 ctggaagaag aagctcatgt agcagaagta ccagttattc cccatgaaac agttgtattt
      721 ttgaaatttt tacttggaca gcttcagcct gaacgaatct tggagatcgg agcagctatc
      781 ggtttttcat caagtgtcat ggccacgaca atcggagaaa acgctcatgt tacgacaatt
      841 gatcgtttcg atgtcatgat cgagaaagct aaaaaaaact atgaacgtct aggactgaca
      901 gataaagtga ctttgcttga aggtcaagca gcagatatct tgccggagtt gtctggtccg
      961 tatgacttta tttttatgga tagtgcaaaa tcaaaatata ttgaattttt acctgaatgc
     1021 ttacgtttgt taagaaaagg tggcgttttg atggtagatg acatcttcca aggaggaacg
     1081 atcttgctcc cagatgaaga gattccccgt ggcaaacgag ctatccatcg taaattaaat
     1141 gaatttcttc gtgttgtgat ggatcatccc gatttgacat cgacaatctt gccattaggt
     1201 gatggtgtga tcttaatgac aaaggaatca gaaacaatcg agttgtgata catgattatg
     1261 gcagcctgta gataatcgat tgaatcgagg gctcaattcg aacttgatgt aaaagtttct
     1321 atatttgtaa gtcaattaaa ggatacagga aatctaaata aacaggagta gcatgaagtc
     1381 aatgtagctc ctgttttcaa ttgataacct tagaacagct gaaggagaat gtctcgtttt
     1441 aaaagattga tttagtagtt gggccaaaat aattcattat ttgctaatta aataactatt
     1501 tcgtattgac agaagcaatt taattatcta taattattga tgttccgctg attcttgtca
     1561 tagtagctca gctggataga gcatccgcct tctaagcgga cggtcggggg ttcgaatccc
     1621 tcctgtgacg ctaaaaaacc gttagaaact gaggtttcta acggtttttc tttgtttttt
     1681 gtgccaatat tgtttgtttt caaaggcaaa taaaataaaa aaacatcctt atcgagtaca
     1741 attataagga gcaaaaaaat aaaactcatc agaaaagagg aagaagatat gacgatacta
     1801 tctagaattt tagtgactct agttgcaatt gaattttttt atatcatgta tatcgaaacg
     1861 gtgagaactg attctgacac gacaagtagg gtttttaaaa tgagtaaaga agagttaagc
     1921 agaaaatctg ttcaaacatt gtttaagaac caaggagtgt acaatggact attaggagtg
     1981 ggactacttt atggagctta tctttcttcc gcatctaaag aaatcacaag tatgctatta
     2041 atatctattt tttttgttgc attatatggc agtttgacta gcgatcgttg gattttactc
     2101 aaacaaggtg ggctagctct gttggcattg ctttctattt tcttataaat atagaaaaaa
     2161 taagaattta tctaacagga aactttgtga aaaatattac tttatctcat gaaatatgat
     2221 attctaatga atggtatttt agaaaatttc atgaaaaagg agttagatag gtgttcaaaa
     2281 attatgtttc ctcactaaaa aaagaatttt caggttatga tctttcaaga tttcagaaag
     2341 atcttttggc tgggatcaca gtagctgctg tagcattgcc tttagccttg gcttttgggg
     2401 tatcaagcgg agctgatgcg gccgcaggtt tgattacggc tatcattgcc ggtatcgtta
     2461 ttggcggttt atcaggcggt ttttatcaaa tatcaggtcc gacaggtgcg atggcagcta
     2521 tcttgatgtc tattgcggca acaaagggaa tgcaaggtgt attgcttgct acttttttag
     2581 cgggtctatt tttacttgca gcaggacttt tccgattagg aacactgact tcttttatcc
     2641 cagcacccgt aattacgggg tttacttcag gtattgctat cattattgca ttaggtcaga
     2701 tcgataattt atttggtgtt cactcggaag gtacaaacgt tattgaaaaa ctaagtagtt
     2761 atcaaacgat aggatttccg ataaacacag cctctttgat catgggaggg ctagtcattt
     2821 tagggatgtt tatctatcca aaaaaatggg cgaaacgaat gcctagttcg ctgctggcta
     2881 tcattttggc aacagcagtt atgctattgt tccatttacc agtagcaact gtgggtaaga
     2941 ttccacaaac attgatcagt agtaatcgtt tgaatatggg agattttagc ttttctgcct
     3001 tgcaacaagt ggcagttcca gctattagca ttgctttatt aggaatgatt gaaagcttgc
     3061 tatgtggtgc ttcggctggc cgaatggcaa ataagccctt agatagcaat caagaattaa
     3121 tggcgcaagg aattggaaat ctgttgcttc ctttctttgg cgggattcct gctactgcgg
     3181 ccatcgcccg tacaagcgtg gccatcaaat ctggtgcaca aactagatta gctggtatga
     3241 tacatgcagt tgtcttactg ctctccatgc ttgtttttgc gccagtgatg tcacagattc
     3301 caatgccagc acttgcaggt gttttgattg tgacggcttg gcgtatgaat gaatgggaaa
     3361 cgatcaagga gctatttact aataaatatt ggtcagcagt tctattgttt atccttacga
     3421 tgatttgtac agttatcttt gatttaagta ttgctatcgt gatcggggtg atcggcggct
     3481 gtgtattttt tgttgtcaaa agcgctgcta tcacgatttc attagaaacg atcgattggc
     3541 aaagaatggg gctgccagaa acggaaaagc tgaataactg gacagtagtg tatattagtg
     3601 gtccactgtt ttttatgtct tcagaaaaac tcaaaaataa attggaagaa ctaaatcatc
     3661 acgatggtgt cattttctcc atgcgaggcg taccaagtat tgatttgacg gcacgtgcag
     3721 tttttgatga atttcaagaa aaagcagagc aaagaggaca aacgattatc tatacttcct
     3781 tgcagccaga agtagaaaaa caactttctc acttgtggga acatacattc acgcatcaac
     3841 atcaaacagt tgctcatgcg ttgacagctt tacatgcgaa atatactgca attagccatt
     3901 aaaacaatga aaaagaacag acaagactgt aaactgaaga ttttatggtc ttgtctgttc
     3961 tttttaatat tgctgtaaaa gtgaagctag tacttgccag tcttgatgga taacaggtac
     4021 aagggagcga ttgtagaaaa tagctgctgg atgaaaaagt ggaagaattc gatagttttt
     4081 ttctgaccat tcgtaggcat ccttttgttc tgttaattgt aagacaggcc ctgtatagag
     4141 ttgaccatga tttttggtaa ccgtatattc tttgccaagt aaacgttgaa gaccaatgtt
     4201 tccaagtgtc gcaattaatt ttggctggac cttcatcagt tcgtaatcca atattggtgc
     4261 atgcgcaaat acttcggaac gagtaggtgt ccgatttgga taaacagttt ctgactgttg
     4321 tgtccgttta ttgatccgat gagtcacgcg atacgggcgg ctccttacag cactggtgat
     4381 atacacctct tctcgtgtta agccagttga agaaagtgcc tgcatcaatt cttttccagc
     4441 ttgaccgcta aaaggaacat gcgtatcaat ctctgttttc cctggggctt ctcccacgag
     4501 catcagttta ggatgaagag gtccttgccc agcgataaat ccttctacag gacggccgtt
     4561 cattcttaaa gaagcttctt gtatcagttc ttttgggtat tccatttatg ctcctccttt
     4621 ttcttttatt ataagaataa aagaaacaca atgcgactat tttatttggt agaactgtga
     4681 ggatagaaaa gcagaagtat tgcttgaatt tttgttttac tttttgattt taaatttagt
     4741 tgactaacgg ttgctgttct ggtaagctag tactgttgta attgtggact ccacagctac
     4801 aaccgctcag aacaagtaat aagcacagca tatgctgtca cttcggatgg cgagtctaag
     4861 tatatcagaa ggaggtgctt tatagcatgt acgcaattat taaaactggt ggtaaacaag
     4921 taaaagttga agtgggacaa gcaatctatg ttgaaaaact tgacgttgaa gcaggagaaa
     4981 aagttgtttt tgacgaagtt atcttagtag gcggagaatc tacaaaagta ggcgctccaa
     5041 ctgttgcagg tgcaactgtt gaaggtactg ttgaaaaaca cggcaaacaa aagaaagtcg
     5101 ttactttcaa atacaaacct aaaaaacatt cacaccgtaa acaaggtcat cgtcaaccat
     5161 atacaaaagt ggttatcgac gcgatcaacg cttaatagcg tttgagaaag aaggcctata
     5221 tatgatcaaa ggaacattta agcgaaatga cgcaggtcag atcgtttcat tcactttgac
     5281 cgggcatgct gatgctggtc cttatggaag tgatatcgtt tgtgcgggtg tttctgcgtt
     5341 agcgatcagc actgtcaacg ggattgcctc attagccggt tttgagccga ttgttgaaat
     5401 gaatgaagaa gaaggcggtt atctttatac cgaagtcact tcaggaatga cacaagaaca
     5461 aaacaatatt gctgagattc tattagaaaa tcttttatta ggattacaat cgatagaagc
     5521 tgaaaactca gaatatatcc aaattaaaac aattaatgat aaataggagg tgcagaccat
     5581 gttgttatct atgaatctac aattatttgc ccacaaaaaa ggtggcggtt ctacatctaa
     5641 cggccgtgac tcagaatcaa aacgtctagg tgctaaacgt gctgatggac aaacagttac
     5701 aggtggatca attctttacc gtcaacgcgg tacaaaaatc tatccaggtg taaacgtggg
     5761 tatcggtgga gacgacactt tatttgcaaa agttgatggc gtagtacgtt tcgaacgtaa
     5821 aggccgtaac aaaaaacaag tgtctgttta tccagtagct caagaagctt aattgaatct
     5881 tatgattaaa gaggttgtga caagactttt gtcacagcct tttttgttta taaaaataaa
     5941 agttgtagtg gaggctacga agtttacttt gtctttttac acttaatatt tttttgccaa
     6001 tttggaatca ggatgttttg ataactatcg ttttcaattg atggaagcta tattgtttgc
     6061 tatcagtgaa ataattatat ggtaaacagt gttctttttc ttgtttccat tataaaaatc
     6121 gtatcgggat ttttcgttct tttaaattga acatttctat ctatccgcgt gttatcaaca
     6181 aaacaaaatc aggtaaatag ttgacgaaac agaaataaat acatagtatt atgttttgaa
     6241 gcctaagtac aatagtctat agaaaaggag cgataccatt ggcaaaacgc attgaccgaa
     6301 tttatatgta tgtcaaagaa aaaaccgctc atttaacaaa ggcagaatat gatcaaggag
     6361 taacaacaca ggaaattgct gaagtgttag ggattcagcg aacaaatagc agtaaagatc
     6421 tgaatcagct agtgcgcgaa ggaaaactga taaagacaga cggacgacca gtacgttaca
     6481 tatatcaaac actcgttacg cacgtaaagc cagttacaaa acatgtagtc agttacaaag
     6541 aaacgccggt ctctgaagaa aaaaatacac tccaaataga tactaaggat atctttgcaa
     6601 aaatcattgg agctaacggc agcatgaaaa attcagtgga acaggcaaaa gctgcgattt
     6661 tgtatccgcc aagaggatta aattgtttga ttacaggacc taccggatca ggaaagactt
     6721 attttgcaca cgcgatgtat cattttgcaa aagcgcatca tgtaatagat gaggaaacgg
     6781 aactgattgt tttcaattgt gctgattatg ccaataaccc tgagcttttg atgagtcatt
     6841 tatttgggta cgcaaaaggt gcttttacag gcgcagatga agaaaaaatg gggattatcg
     6901 atcaagcgga tggcggtatg ctgtttttgg atgagattca ccggttgccg cctgaaggtc
     6961 aagaaatgat cttttacttt atggatcatg gaacttacag tcgtcttggg gaaacaacga
     7021 aatcccatga agcaaatgta cggatcgtag gtgcgaccac agaagaccca ggttcttctc
     7081 tattagaaac ttttgttcgg cgtatcccaa tcaatatcaa acttccggct tttgaaaaac
     7141 gtcctgcaaa tgagaaagtc gatttagtaa aaatcatgat tgcccacgaa gcaaaccgca
     7201 cgcagcgaaa gatttctttg acagaagatg tagcaaaagc attgatcggc agtgtgacct
     7261 atggaaatat aggacaattg aagtcaaata tccagttagt atgtgcacga ggatttctaa
     7321 atcatatgca atcacccgaa atctcgatca caatcgatga tttgacagaa gggattcgta
     7381 gcggattgat tcaattagca agcaaccgca gtgcaatggc agagctttct aaaatattgg
     7441 agccaaagat aactgtagca ccaaatgata cgatcatgaa aatccaatcg gactcctatg
     7501 agttacctta taatctatat gatatcatcg gagataaagc agctttactg aagtcggatg
     7561 gtttggatca agaagcaatc aatcatttca tttctacaga tatcaacatc catctgaagt
     7621 ctttctataa agaccatggt ttttctttta atgcagacag caaattagca gaatttgtag
     7681 atccgaaaat catagaagtg accaatcaga tttatgtaat ggtcaaaaga tctttaccat
     7741 atgagtttca gcaaaacttt atttatgcta tgagcttgca tatcagttct tttttgaaaa
     7801 aaatcaactt aggagaagaa cggcatacga atgataacat ccgtgagatg gcttttgatt
     7861 ttccagatga atatgctgtt gcgaaagaag tgcgtagtta tatcgaaacg tattttcaag
     7921 tggagattcc agaaagtgaa gactattact tgaccgttct actcgtctcg ttacgggcaa
     7981 atcaagcaag cggcagaatc ggtgtagtcg ttgcagctca cggaaacagc acagcaagca
     8041 gtatggtcca agttgtccaa cagttgttag atgcagagaa cgtacgagca gtagacatgc
     8101 ctttggatat ggatccgaaa acagcattgg accgaattga acgcaatgta caagaagtag
     8161 atgaaggaag cggggtcata ctcttagtag atatggggtc ccttgcgtcg tttaattcgc
     8221 aaattcaaag agacactgga attccagtac gtactgttga tatggtaaca acttctctag
     8281 ttttggaaac tgtacgaaag gtctcagtat tagggacaga tctagatatg ttgcatgaat
     8341 cattgaaaaa ttttcgaggt tacgcagaaa tatcttctgt agaatctgtt caggaagcaa
     8401 aacctattca taagaaaaaa gctgttttgg ctatctgcgc atctggcgaa ggtacagcac
     8461 aaagaatcaa agaattgatc gaacgagcgg taaacaagcg tcaagaaaca gaattagaag
     8521 ttctagcttt gtcgattgtg gaagtgaaag aagaactgcc agttatccaa aaagaatatc
     8581 agatcatagc tactactggg attactgatt tgaatatcca agccccattt attcctttag
     8641 aacgttttat tgatcaaaat atcgaagtca ttttggatca gttgctgatg gaatcagaat
     8701 tggaggaaac tgaattcatc tccctagatg aagagtcagc taaaaatacg tgtgttgagt
     8761 ttatctctga taacttcatc tttattaatg gtagtaagtt aatcgatcca atgtggcaat
     8821 tttctacaca aatctctcag acgacgggaa tcggcgatga agaatatgga ttcaaaatca
     8881 acctagtgat gcacacagca ggaatgatcg aacgtattat tcggaatgaa ccattgactg
     8941 tggaagaaaa cgaattgaca aatacaacga atgatcctct ttatagccaa ttggcagcct
     9001 cagttgttct gttggaagat cagataaaag taaaagtgcc gatagaagaa atgtattatc
     9061 tgctacgtct agtccataat cagttagata aaaaagaata cactgtgcca tgaatacaca
     9121 aaaacgatac acttttatag tgtatcgttt ttgtgtattg gttaagaatc ctttaaattc
     9181 aattagtgta tcgtaaaaaa gttggcacgc tttttgctta tagtagtgtg tgctaggagg
     9241 tggttgttca cacagaaatg ataggggttt ctgtgaagta gtgaggaaga aggagttttg
     9301 cagatggata ttcgattggc acgtatagat gatcgtttaa tccacgggca agtcgcaaca
     9361 gtttggtcga agcaaacagg tgttgaacgt atcattgtga tcagcgatgt agtcgctcaa
     9421 gataaattga gaaagtttct actaaaagaa gctgcaccgc cggggatcac agcaaatgta
     9481 ataaccgtgc agaaaatgat tcaaatctat catagtggat tattatcacg agcaaaagtg
     9541 atgcttttat tcaccaatcc ccaagatgtt gaagcggttg tccgctgcgg tgtacagttg
     9601 aagtcgttga atatcggcgg tatgagtttt actgaaggca aatcaatgat taccaatttt
     9661 gtttcagtaa atcaacaaga tattgattcc tttcgttact tgaacagtca agaagtggag
     9721 ttagagattc gaaaagtacc tgctgacagg aaaatccttt tgatggaact actagcgaaa
     9781 atcaaaaagg tgtagcaaga atggttgttg gaaaaggtca aataattctg ttgtaatggt
     9841 tagcaattat taatattttt gaatttgtta caacaaaagt aaataggagg tataaatatg
     9901 gtagggatta tcctagcaag tcatggacaa tttgctgaag gaatcttaca atccggttca
     9961 atgatctttg gcgaacaaga aaacgtcaaa gccgtgattt tgaaaccaag tgaaggtcca
    10021 gatgacttga gagccaaatt ggaagaagcg gttgcttctt ttgataatca agacgaagtg
    10081 ttattcttag ttgatttatg gggcggaaca ccgttcaatc aatcaaacac attattcgaa
    10141 gagcacaaag ataaatgggc aatcgtcagc ggtttgaatt taccaatgtt gattgaagcc
    10201 tacgcatcac gcttttcaat ggaatctgct catgaaattg cagcacacat cattgaaaca
    10261 gcaaaagacg gtgtaaaagt aaaaccggaa gaattagaac cggcagaagc accgaaagca
    10321 gcagttgaag acgcacaacc taaaggagca cttccagaag gaactgtggt cggagacgga
    10381 aaaatcaaat atgtattagc acgtgttgac tcacgtctgt tacatggaca agttgcgaca
    10441 gcttggacaa aagcagtgca accaaaccgt attatcgttg tttcagatgc agtatcaaag
    10501 gatgatctgc gtaagagact aatcgaacaa gctgctcctc caggtgtaaa agcaaatgtt
    10561 attcctatta gcaaaatgat cgaagtagca aaagaccctc gttttggaaa taccaaagca
    10621 ttgcttttat ttgaaaatcc tgaagacgta ttgacagctg ttgaaggtgg cgtagacatc
    10681 aaagaattaa atgttgggtc aatggcgcat tcggtcggta aagtcgttgt aagtaaagta
    10741 ttgtctatgg gacaagaaga cgttgaagcg tttgagaagc ttgaacaaaa aggtgtgaaa
    10801 ttcgatgtac gtaaagtacc taatgattca cgcgataaca tggatgatat tttgaaaaaa
    10861 gcaaaagctg aattagcaaa agcgtaataa ataatattaa aatttatagg aggcttatca
    10921 tgtctattat ttcaataatt ttagtcgtat tagttgcctt tctagctggt atggaaggta
    10981 ttcttgatga atttcaattt catcaaccac tagtggcatg tacattgatc ggtttagtta
    11041 ccggtaacct cgaagctggt atcgtacttg gcggtacgct tcagatgatc gctttaggtt
    11101 gggcaaatat cggtgccgct gtagcaccag atgctgcatt agcatcagta gcatcagcaa
    11161 ttattttagt tttaggtgga caaggtgtta aaggtgttcc ttctgcgatc gcgattgccg
    11221 ttcctttggc tgttgcaggg ttgttcttaa caatgatcgt acgtacaatc gctgttccaa
    11281 tcgttcattt gatggatgcg gctgctgaag aaggaaacat ccgtaaggtt gaaatgtggc
    11341 acatcattgc tgtatgttta caaggggttc gtatcgcggt cccagcagca gcgttgctat
    11401 ttatcccagc acaaactgtg caatcattcc ttgaatcaat gccagcatgg ttaacagatg
    11461 gtatggctat cggtggggga atggtcgtag ctgtaggtta cgctttggta atcaacatga
    11521 tggctacaaa agaagtatgg ccattcttcg ttattggttt cgttgtagct gcaatttctc
    11581 aattaacatt gattgctcta ggtgctttag gtgttgcatt agctcttatt tacttgaacc
    11641 tttctaaaat gggtggttca tcaaataatg gaggcggaag caactctggt gaccctttag
    11701 gcgacatttt gaatgactat taatcttgaa ggaggaaaat aaaatggcag aagaaaaaat
    11761 caaattatca aaaaaagatc gtttagctgt tgcgtggcgc tctaccttca ttcaaggttc
    11821 atggaactac gaacgtatgc aaaacggtgg ctgggcattt tctatgattc cagcaatcaa
    11881 aaaattatac aaaacaaaag aagaacgttc tgcagcatta aaacgtcact tggaattctt
    11941 taatacacac ccatacattg cttctccaat tttaggggtt acattggctc tggaagaaga
    12001 acgtgcgaat ggcgcgcctg ttgatgatgt agcgattcaa ggggttaaag taggtatgat
    12061 gggtccttta gctggtgttg gtgacccagt gttctggttt acagtacgtc caatgctagg
    12121 tgcactaggt gcttcattag ctatgggagg aaatatcctt ggaccaatca tcttcttctt
    12181 aggttggaac attattcgtt gggcattcat gtggtataca caagaatttg gctacaaagc
    12241 tggttctaaa attactgatg acctttctgg cggattgtta caagacgtaa caaaaggtgc
    12301 ttcaatttta gggatgttcg tattagctgc ccttgtacaa agatgggtat caatcaaatt
    12361 cttaccagtt gtatcaactg taaaattaga taaaggtgct tacatcgaat gggataaatt
    12421 gccttcaggt ggtgaaggaa tcaagagtgc atttgaacaa gttaataatg gcttagccct
    12481 ttctcctgaa aaggtaacaa cattacaaga caacttagac caattgattc ctggtttagc
    12541 tgcattagga ttaacattgt tatgtatgtg gttgcttaag aaaaaggttt ctccaatcgt
    12601 tatcatcctt ggtttattcg tagtcggtgt tgttggacac gtcatcggac ttttataatc
    12661 cataaaaata tagctcgggc gttgtcccga gcttttttta tatagtatac tgttcataaa
    12721 caaatatctg gagggagaaa atggttcaat caatcaatac aaaagtagac ttggtaattg
    12781 atgctacttc gtttacgggt ctgacagatt acgggcaaat catgataggt gataatggtt
    12841 ttgagtttta ccatgcgcga gatagacgca aatatatcca aattccatgg gaagaagtgg
    12901 attatgtact agcatcagtc atgctaaagg gaaaatggat tcctcgatat gccattcaaa
    12961 cgaaaaaaaa tggtacatac acattttctt ccaaagaacc taaaaaagtg cttcgtgcga
    13021 tcagagaata tgtagatgaa gatagaatgg tacaatcatt gagtttcttt gatgtagtga
    13081 aacgtggtat tcgtagtatg tttaaacgca agtaaaaaat aaagaaaact tctaagagga
    13141 ggttttcttt atttttttac tttgcagaag aaaatatgaa atagcaaaac caattatttg
    13201 tcccctttaa atcccatatg ctaaaattga ttagcaaaga aggaggttat attatgagtt
    13261 ggttatggac attgattgtt ggagccgtga tcggagcaat tgcaggtgcg atcaccagta
    13321 aaggaaattc gatgggctgt gtaacgaata tcattgctgg actagtcggt tcaagtattg
    13381 gacaagcgat tttcggcgaa tggggaccaa aagctgcagg tatggcactt attccatcta
    13441 ttttaggcgc agtgattttg gtagcagttg tctcattttt ctttggaaaa cgatcataag
    13501 tttttacaaa atagtaaact aaaaggagtt gcagcgagac tgtgactcct ttttggcttt
    13561 atttatttaa aataaaaaga agttttccgg aaatttttat ttaactgtcg tgtacgaaga
    13621 aaagtttgct ataatggaaa aaagcgtgca aagaaagagg gagtttcatt atgatgctac
    13681 gtgtagaaaa attgcgcaaa aaaatgcaag aagaaaattt ggattcattt ttggttacca
    13741 gtccgtacaa tcttcgttac ttaacgaact ttacaggtac gacaggatta gctgttatta
    13801 ctttggaaaa agcatttttt attactgatt ttcgttatac cgagcaggcg gcagcgcagg
    13861 ctcaagggtt tgaaattatt aaaaatgtcg gacctatttt tgatgaggta gcaaacttag
    13921 tacgcaaaga agaactgcac tcattagctt ttgaggaaac aacagtctca tttttagaat
    13981 attcggtttt agaagaaatc atcgatgcgg aattagtacc tgtttcacag atgatcgaag
    14041 aactcagaga agtgaaagat gaagaagaaa tcgctattat cgaaaaagca tgtcatattg
    14101 cggatatggc atatgatcac atcctgaaaa tgatccaacc gggcatgact gagattgaag
    14161 tcgcaaatca actagacttt tatatgcgtt ctcttggcgc ttcaggtgtt tcttttgata
    14221 cgatcgttgc tagcggattg cgttcggcta tgccgcatgg tgtagcaagc aaaaaaatca
    14281 tcgaacaagg tgacttgatc acattggact ttggttgcta ttacgaaggg tacgtttccg
    14341 atatgacacg tacattcgct attggtgatc ctggggaaaa attaaaagaa atttatcaaa
    14401 tagtcttaga tgctcaatta gcagttattg atgctgcaaa accaggaatg actggtgtcc
    14461 aattagatgc agttgctaga gattatattt caaaacatgg ttacggggaa gcatttggac
    14521 attctacagg acatgggatc ggtttagaga tccacgaagg accaaacgtt tcttctcgtg
    14581 cagaaaagca atttgtacca ggaaatgtga tcactgatga accagggatc tacttaccag
    14641 gaatcggtgg agtacgtatt gaggatgact tgttgatcac agaagatgga aatcgtgtgt
    14701 tgactcgttc accaaaagaa ttgatcatct tatagtttca tcaatagaag aagtaagcgt
    14761 aaaaatcagt gttcacagac tttgttatag aaaatcactc gatttaatga tacactatag
    14821 aagaactttt aagctgtaag agataataaa gttgggtgtt ttctccaatt catagcagtt
    14881 tgcagctgat cggcggcagt tttcttacca cgtgatcact atgccgcatc atagagaatt
    14941 cggatgtatc tcgtgcttag gcaaagggag gaataaataa atggcagatg aaaaaaattt
    15001 gattttaaac gcaaaccaag atttaggaga gatcgtgatc gctcctgaag tcatcgaagt
    15061 aattatcggg attgcagctt ctaaagtaga aggcgtatac ggaatgcgcg gaacttttgc
    15121 cagcaatgtc actgaattat taggacgtgc agctcacgga aaaggtgttt acttagcaaa
    15181 cgaggaagat ggcttgaaag tagatcttta ctgttatcta gaatatggtg tttcagttcc
    15241 taaagtagca atggaaatgc aggaacgggt cagccagcaa gtccttttca tgacggatat
    15301 cgatttatct gaagtcaata tccatgtagt agctgttgtt cctgaaaaat tagaacaacc
    15361 aacaatcgaa gaactttttg aagacgaaga ggaagagaat gaataaagaa ttatcaagac
    15421 acgagattcg ggaaatggca ttgcaggctt tatttccatt agacttcaat gcagacctaa
    15481 caaaagaaga tgcgattttc aacgcaatcg aattagacca ccgcgacatg atcaacgaag
    15541 atgaatcaga gtttgttcct gtttatcttg atacgctagt tggcggtgtc tgtgcaaaaa
    15601 aagatgaatt ggataaagtg attgaaaaac atttaaaaaa caattggcac atcaatcgga
    15661 tctcaaaaat ggatttggtg atcttgcgta tcgcgatttt tgaaatgact tatgtgacag
    15721 atgtaccagc ggctgttgct ttaaatgaag caatcgagtt ggcaaaaaca tttagtgatg
    15781 accgttcacg taaattcgtc aacggtgttt tatccaatgt attgaaagaa ttagaagctg
    15841 gggcgtaagt cctagctttt ttctatgaat catcataaac ctgttataaa tcgggaaaat
    15901 tctatggtag aatgaaaaca caaaaaatat ctgggagtgg ttagatggca gaattgatta
    15961 acggaaaaga attagctgaa aaaatgcagg cagagattgc tgaaaaaatc caaaaattga
    16021 aagataatgg tatccatccc ggattagtcg ttttattggt aggggaaaat ccagccagcc
    16081 aaatctatgt aagaaataaa gaacgtgtag caaaagaaat cggaatccat tcattggtag
    16141 agcgttatcc tgagacgatt tctgaagacg aactacttgc agaaattgaa aaatacaatc
    16201 aagatcctag ttttcatggt atattggtcc aattaccatt gccaaagcat atagatgaag
    16261 aaaagatcct gttagctatc cgccctgaaa aagacgtaga cggttttcat ccgatgaatt
    16321 taggcagact ccttgcgggt aaaccggaca agattccttg tactccatac ggcatcatga
    16381 aaatgtttga agcttattct atcgatccgg cgggtaaacg agcagtagtt atcggacgaa
    16441 gcaatatcgt cggtaaaccg atggctcagc tactcttgat ggcagatgca actgttactg
    16501 ttgctcattc taaaaccgaa aatctggctg aactggcaca agaagcagat attttagtcg
    16561 tagcaatagg acgtggacat tttgtcacaa aagaattcgt aaaaccaggt gctgtagtga
    16621 tagatgtagg gatgaatcgt gatgctaacg gtaagctgat cggtgatgtg aaatttgatg
    16681 aagtggagcc tattgcaggc tggatcactc ctgtgccaaa gggtgtaggg ccaatgacta
    16741 tcacgatgct gatgtatcaa accgtaaaat ctgccgaagc ttttggagca ggtgaataga
    16801 tggcacaaga atatttaact gtcactacat taacgaagta tttgaaaaga aaatttgatg
    16861 ccgatcctta tttagaacgc gtatatttaa caggcgaaat atcgaatttc cgtttacgtg
    16921 ccaatgccca tcaatatttc agcttaaaag atgaccatgc aaaaatatct gctattatgt
    16981 tcaaaggagc attccaaaaa ttacgattcc agccaaaaga aggaatgaaa gtccttgtcg
    17041 ttgggcgtat ttcattatat gaagcgggag gttcttatca aatctatgta gagcacatgg
    17101 agcctgatgg aataggtgct ttatatcaag aattggaaga acgaaaagaa aagctgatga
    17161 aggaaggcct ttttcaaggg cctaaaaaaa tgctgccacg tttcccaaag cgaatcgcgg
    17221 tccttaccag tccaagcgga gcggttatcc gcgatatcat cacgacggtg aaaagaagat
    17281 atccaattgt ccaattggtt ttgttcccaa cagttgtaca aggaaaccaa gcagcggaca
    17341 atgttgtcag gaatatccag agagtagaag agatgggcaa tttcgataca atgatcatcg
    17401 gtcgtggggg tggttcgatt gaggatttat ggccgtttaa tgaagagcgg gttgctcggg
    17461 cgatttatca agctaatacg ccggtgattt catctgtagg acatgaaaca gataccacga
    17521 tcgctgattt agtagcagat gttcgggctg ccactccaac agcagctgca gaattagctg
    17581 ttcctgtttt gtcagaagag ttgatgcgca ttcaagaacg gcaagctaga ttgcagcagg
    17641 cgtttgtacg ccagatccag cgcaaacaag aacggttcga gcgtgctcga cgttcctatg
    17701 tatttcggca gcctgaaaga ttgtatgaag gtcagaccat caaattagac cagttgaaac
    17761 agcgattata tcaagctaca cagcaaattt atcatgcgaa agaaaaacaa acgatcgcat
    17821 taagccacca attggaacaa gtggctccgc tttatcgagt taaagcagct cgccaagaaa
    17881 ctgcttatct agaaaaacgc ttagaagaga aaatccaaca atatatgaaa caacagcagc
    17941 agcgttttgc tcaagctgtt caatctcttg atttgttgag tccattgaaa atcatgggaa
    18001 gaggctacag ctatacgacg ttaaatgatc aagtagtaaa aagtgtcacc gagattcatc
    18061 aaggggattt gctgacgatc cattatcaag atggaatcgt taaaggacaa gtacaagaga
    18121 tagacaaagg agaataaaaa tatggaaaat ccaacttttg aagagtcact gcaagaatta
    18181 gaaaaaattg tcatgcagtt agaacaagga gacgttccat tagaatcagc tctagattcg
    18241 tttaaacgag gaatggaatt aagcaaacat tgccaagata cattagctaa agcagaagag
    18301 accttgacga aaatgatgac agaatcaaat gaagaagttg attttgacgg aaacgaggaa
    18361 agctgagatg agattaactg acttttcagc tattcatcta cctgctgtag aaaaagaaat
    18421 cttgtctttt ttagatgagc atacaacgga tagaggactc tatgatgcga tgagttattc
    18481 agtgaaagca ggcgggaaaa gaatacgtcc cttattactt ttaacggccg ttgcttcctt
    18541 tgatgagcct attgatgttc ccgtctatca agtagcagct gctttagaaa tggtgcatac
    18601 gtattccttg atccacgatg atttgcctgc gatggacaat gatgatcttc gtagaggaaa
    18661 acctaccaat cataaagtat tcggcgaagc attagctata ttggccggag atggtttgct
    18721 tacaggagca ttccagctaa tcagtatggc acacttaggt aattctccta agcttctgct
    18781 gcttcagcaa ttagctgttt gtgcgggatc acaaggtatg gttgctgggc aagctgcaga
    18841 tatcgagggc gaatcgaaaa agctttcttt agaagaatta gcatttattc atgaaagaaa
    18901 gacagggcat ttgattcgtt atgccttgct ggctggtggc atattagcga agcaacctga
    18961 agaaatctta ttgctacttc aacgattagc cgaacattta ggtttagctt ttcagataag
    19021 agatgatcta ctcgatgtga ttggtacaac gaaaacatta gggaaaacag ctggtaaaga
    19081 tgaacgtatg gaaaaaaata cttacccgcg tttattaggc ctagaaaaga cacgtgaggc
    19141 gttggagatt gagctgttat ccgccaacaa gatcatagac aaattagaag aggacgtttt
    19201 ttcttttgat ggcgggctga tgcgccagat gatcaagcaa tttgatgtgg agaaatgagg
    19261 gaaagaatgg aaaaagaacg cgtagatgta ttagcggtca aacaaggatt atttgaaaca
    19321 agagaacaag ccaaacgttc agtgatggct ggtttgattt acaacgaaaa aaacgagcgg
    19381 tttgataaac cgggagaaaa aattccagtg agtagtgaat taagagtcaa aggtaaaaaa
    19441 cttccttatg tttctcgagg gggattaaaa ttagaaaaag cactcaaagc atttgatcta
    19501 tctgttgaag gaaaaacatt gttagatatt ggtgcatcca ctggcggatt tactgatgca
    19561 gctttgcaaa atggcgcaaa aatgagttat gctttagacg ttggctacaa tcagctagct
    19621 tggaaaataa gacaagatcc tagagtcgtc gtaatggaaa gagtgaattt tcgttatgca
    19681 aaacccgaag actttacctt aggaattcca gaagttgctg ttatcgatgt ttcttttatt
    19741 tcactaaaac tgatgctgcc tccattacat gctattttaa aagaagaggg agaagtgatt
    19801 gctttaatca aaccccaatt cgaagcaggc agagaagctg tagggaaaaa cggaattgtt
    19861 cgtgaccctc agacccatca aaaagtttta gaagacattt tgtcatttgc ggcacaaggt
    19921 ggatatgatg tgctggaatt aagttattct ccgatcacgg ggggagaagg aaatatcgag
    19981 ttcttggctc acttgaggaa agtacccgaa agcggaacaa tcaattcagc tattaatatg
    20041 gcagaagtgg tttcaaatgc acacgaacag tttgaccata aataaattgt aactgtgaaa
    20101 atcaagtgag aagtatgcgg attcttcttt attataccaa ggaaataagc tatcattgat
    20161 taagaagaat atgctaagga gcgaagacgt aatgagaaaa aaagatcggc atcgtttgat
    20221 tactcgattg ttgagtgagc aagatatccg aaagcaggaa gaatttgttg aaatcctgaa
    20281 aagcaaagga atatctgtta ctcaagctac gatttccaga gatataaagg aactgaaact
    20341 gatcaaggta ccggcttcag atggcggcta ccgttatagc ataccagctg aaacaaacga
    20401 agatgtaaat actaaattgg aaaaactatt aaaagacgct ttcgtggcag ttgaccaaat
    20461 ggaaaaattt gttattttga aaacattgcc gggaaatgct tcggcagcag caaatttgat
    20521 tgataaacgt tacaagaaag aattattttc gattatcaat gatgacgata atgttttgat
    20581 gattacacga actgaagacc aggcagtttt attgaagaaa gacttcctgc attaccttta
    20641 agttgaggag cgaatgtgat gctacaagaa atcagtatta ctaactttgc gattattccg
    20701 gaattgcggc tttcttttca cgaaggaatg accgcgctga ccggagagac tggtgcgggt
    20761 aaatccatta ttatcgatgc tttaggtctg ttagcaggag gaagaggttc aagcgattat
    20821 atacgtcaag gagcagaaaa atgtgtgctt gaagggttgt ttgaactacc aaaacaagaa
    20881 ggattctctg aactgatggt tgaattaggg atcgaaacag atgaggacaa tctgattgtt
    20941 cgacgggata tgtccttaac tggaaaaaat gtttgtcgag tgaatggtca tattatcaca
    21001 ttagctaatc tgagaagaat cggcagttat ttagtagata tccaaggaca aaacgaacat
    21061 caggaactgc tccagcctga atcacatctt gctttattgg atcgttttgg agatgtagct
    21121 ttccaacaga agaaaaagag ttatcagcaa gaatatatct cttatcgtga gctggaaaaa
    21181 agggtacgaa aagtgcagca aaatgaaaaa tcctatgttc agagaataga tatgctccat
    21241 ttccagcaag aagaaatagc tgctgctgat ttgcaagtcg gagaagaaga gaaattaaaa
    21301 gaagaaagag aaaaacttag caattatcaa aaaattgctg atggattagc tgcaggttac
    21361 ggagcactga cgaataatga acaaaacagt gtagatggtg ttggcttggc cgtttcagaa
    21421 ctgcaaggga tcgcccatct agatgtggag tatgaagcaa tttatgaaaa tatccaaagt
    21481 gcttactatt tgctgcaaga tgcaatcggc gatatgagtc gtcagattga tttattggag
    21541 ttagatgaga gtcgtttaga agaagttacg caacgtttag aattaatccg acagttgaaa
    21601 cgtaaatacg gcgaatccat tgagtcaata ttggcttact atgacgaaat caccgaagag
    21661 ctagcttctt ctgatttttc agaaggtcaa ttggacaaaa tgaaagagga actagagcaa
    21721 aaagagcttc tacttcagca acaagccgca gatcttcatg aagcgcgtaa aaaaatcgca
    21781 aaagaattgg aaaaatccat tctgcatgaa ttgaaaagtc tgtatatgga aaacactgag
    21841 tttgaggtac gtttcttaaa agaagaaaat agacaattga atcatgatgg atttgaccaa
    21901 atcgaatttt acattacgac caatccagga gagccactga agcctttggt gaaagtcgct
    21961 tctggtggcg aactgtctcg gatgttgctt gctttgaaaa ctattttttc ttctgagcaa
    22021 ggtgtgacaa gtattatttt tgatgaagtg gatacaggtg tgagcggaag agttgcacag
    22081 gccatcgcag ataaaatttc tcaaatatcg aaatactctc aagttttgtg catcactcat
    22141 ctgcctcaag ttgctgcagt cgccgactat caatattata ttgtgaaagc agtgattgat
    22201 ggacgtacgc aaacttctgt ttcagagttg aaaacaaaag aaagagaaga agaaattgca
    22261 cgtatgctgg ctggaagtga gattacaaaa ctcacactcg aacatgcaaa agaactattg
    22321 aaactcgcaa aaaggacctg agccacctcg cagtggttca ggtccttttc agcagctgat
    22381 agctatattt tatttttaca tgacactcat tacagcagtc aatacaacag atccaatcgt
    22441 taaaatcagc attgtccaaa tgacaaattt tgttacttta gcaaaagtac ttgtttgttt
    22501 tttttcacgc attgttttca acctcgtcat taattagttg attcaagtgt acaatctttt
    22561 ttccatttcc acaagagggc ttttttataa aaacttccct ttttttaaat aataggtagt
    22621 tacgatacaa ataaataatg ttaatagaac tagccatata aaggcatcat ccctttcagc
    22681 aataggtaac ttaatgttca ttccatagat actgccgacg attgtcggta tagttaaaat
    22741 aatcgttaaa gaagttaaaa ttttcataat attattcaga ttattcgata cgattgctga
    22801 aaaagtctga ttcatttgtt ctactagttt taattgaatg cgagaagtag tcattgcctg
    22861 ctctgtttcc accaatacat cacgtaaatg atttttcaaa gcatgatttt cttggaaaag
    22921 tttattttgc gctagttctt tcaatgcatc taaattagca gaagtcgctt ctctgaaata
    22981 aacgaggctt ttttggatat ccatgatttg ataaagttgt ttgttttcag tggatacttg
    23041 aagtttggct tccaaagtat cgcattgaga ttgaagcgta gctaaataac tgttgtaaga
    23101 gagtgtgacc tgccaaatga attgaaagaa gatattgaga ttaggcgaaa tatcactttt
    23161 tggaaaggtc tggctaaaga ctttctttaa aaacagtggt tcatgattcg taattgtgat
    23221 aagcttcttt ttgggcgtca aaataataga gagcgggtat gtatccactt gaaaataacc
    23281 gctaggactt gttttagcaa aaggatattg taacaataat aacgctggct ttgtcaactt
    23341 tgattgattg aattcttctg tacgggaatt ttctgcatcg tccaatatgg aagtgatgta
    23401 atctgacgga atttcaaatt gatgtgtcag ctgatgaatc tcttcttgat taggacgttc
    23461 tacacaatac cagatcgttt ggtcagtttc ctttccggta ttacgcagtt tctcattatc
    23521 taatgtaaaa tatttgatca tgattttcct cctgttgcct gtttctttta ttataacgga
    23581 tagggaattg gaatacatgg aatgtgtttg taagatatag aatataactg cataaattta
    23641 aataaaatga aacagttgtg taaagtatat acagtatact tgaagcaacg ataaaaaagg
    23701 agtttacgtt catgggcaca ttcacaccac ttatcttgtt ttggtatatt tacccggtaa
    23761 tcgttttgtt tggttgccaa tttcttgtaa aattattttc gttaaatcgt cgttttaaaa
    23821 taaaagcccc tgatttggcg attccttttt tgtggggcgg tatgcatgca ttgtctagaa
    23881 atacagggac aatttctatc ttaccgtttt tctttatttc tgttttgcta atgggaattt
    23941 gtattgctgt tttccaagca tattattatg aagaaattat ttatccgcgt tatttcaaga
    24001 tgacttggcg tttggttttt ctgttgacaa tcgtcttgta cgctgtcttg atcattgtga
    24061 acatactttc ttacctatga aaaaaataga aaagctgtaa aaagtctttt aaaaggcttt
    24121 ttgcagcttg atttttaaaa aaatgtttat gaataaaaaa agttgttccc ggatttccca
    24181 gaaaaggtgg tagaaagtgg gggattgtgg tagactaatt ttagttagtg gaggaatggg
    24241 gggctttcaa tgttgatggg tgaattccaa cataatattg atgcaaaagg acgtttaatc
    24301 gtgccctcaa aacttcgtga agaacttggc gaaaagtttg ttctgacacg cggattggat
    24361 ggctgtcttt tcggctatcc gatgagcgaa tgggaaaatc tggaagctaa actgaacgaa
    24421 atgccgttgg ctaaaaaaga cgcacgtact tttgtgcgat tcttctattc ggctgccact
    24481 gaatgcgaac ttgataaaca aggacgaatc aatataccca gcacgttaag aaattatgca
    24541 gctttgacaa aaggttgcgt agtcatcggt gtatctaacc gaattgaaat ttgggatgag
    24601 acacgatggc aagatttttc tgcagcagca gaagagaact ttgacgaaat tgctgaaagt
    24661 atgattgatt tcggactata agaataggaa gaggctgact aatgactgaa acatttcaac
    24721 actatacagt catgttgaaa gaaacggtag atggattgaa catcaaaccg gatggcgttt
    24781 atgtagactg tacactcggc ggtgccggac atagtgaata tttattaagc caactaagtt
    24841 ctaaagggca tctgtatgcg tttgaccaag atcaaaaagc aatcgatcac gccaagctgc
    24901 gtttagcttc ttatgtcgaa caaggtatgg tcacatttat caaagcgaac ttcagagaat
    24961 tagaagaacg cttgagtgag catgtagata aggtagacgg cattttgtat gatctagggg
    25021 tttcctctcc tcagctagat gaagcagaac gtggatttag ttatcatcaa gatgcaccgt
    25081 tggatatgcg catggatcag tcagctcctt tatccgctta tcacgtaatt aatgaatatt
    25141 cctaccatga attagtaaag attttcttcc gctatggcga ggagaaattt tccaaacaaa
    25201 ttgctcgtga aatcgaacga gtacgaaaaa caaaaccaat ccaaacaact ggtgagcttg
    25261 tcgaaatcat taaacaagtc atcccagctc ccgcaagaag aaaaggcggg catcctgcaa
    25321 aaagaatttt tcaagctgtg cggattgcag taaatgacga actcggtgcc gaagaggcct
    25381 ctttagaaca ggcaatccgg ttattgaaag tcaatggaag aatcagcgta atcacattcc
    25441 attcattgga agacagaatt gtcaaaagta tgtttaaaga atatagtacg gtacaagatc
    25501 taccaccggg acttcctgtc atacctgacg aattccaacc agaactgaaa gtcattactc
    25561 gtaaaccaat ccttccttca gaggaagagc taacagaaaa caatcgatca cgcagtgcaa
    25621 agttacgtat tgcagaaaaa ataagaatga aagaggagta gaatgatggc agaattaaag
    25681 aaaatgcaag attacgaata tgatttacct gaattggtgg atcagccgga acaacaaatg
    25741 cctgattctt cttcgaatca agaaatcatt cttccacaat caccggcaaa acgattaaag
    25801 cagatttctg ctttggaaaa aattgctgtg gcaagcgtgg cgattgctat tgttgcttta
    25861 tgtgttttaa cagttatgct gcgcacaaat atcagcggag ctgaaaaaga aatcacgtca
    25921 atacaaacag aaatcaatga taaaaagcaa gaaaaaacga gtttagaaca agaaaaaaac
    25981 gaactttctc gtactgaacg gattaaacaa atcgcagaag aaaaaggatt gtctattaat
    26041 gacgacaatt taaggaaagt gaagtaaatg agtcttaaaa ataaaattcg gcggtttatg
    26101 gaaaagaaaa atctaaatcc gatgaacaat cgcaaaaaag taggtatcat tttatttgct
    26161 acgagtattg gattgttctt tttatttgcc gtccgtttct cgtatatcgt tattggaggt
    26221 catgttgctg gtacctcatt agctgaaaaa acaaaacagt tgtaccaagg aagtgaagta
    26281 gtaaaagcaa aaagaggaac gatttatgat cgtaacggtg tggcgctcgc agaagatgcc
    26341 tcctcttatt caattaaagc gatactctca aaaacatata cctctgggga taaaaaactg
    26401 tatgttgaag aaaagaattt tgataaaata gcagaaatcc tgcataagaa tctttctata
    26461 gacaaaaaag atgcactgaa tattttagag gacggcgcaa aaaaagagct gtatcaagtt
    26521 gaatttggta gctatgggaa aaatatctcc caagaaacca agcaaaacat cgaagcggac
    26581 atgaaaaaag aaggtgtcgc tggtctttat tttgtcgacc atcaggctcg gatgtatcca
    26641 aatggcgtct tttcttctca ttttattgga tacgcagttc cagacaaaga tgagaatggc
    26701 ttagttggta aactaggttt ggagtctgct tacaatgata tcttgagcgg taaagatggt
    26761 aagattattt atcaaaaaga taattttcaa aatcctctgc caggtacagt ggcagaagaa
    26821 gaaaaagctg tcgacggaca agatatctac acgactttag acagcagact acaaagttat
    26881 ttagaaacac tgatggatca agtaaatgaa gaatatcagc cagaagaatt gacagcagtc
    26941 ctgatgaaag caaaaaccgg agaaatcttg gcaatgggac aacgacctac tttcaatcct
    27001 gaaacaatgg aaggattgac tggaaaagat gctatctggc gtaatttctt ggtacaagat
    27061 agttatgaac caggatcaac catgaaagtt ttcactactg cagctgctat cgaagaagga
    27121 gaattcaacg agaatgaaac cttccaatct ggaaaaatcc aagtagaaga tgctacgatc
    27181 aatgaccatg actttggtga aaaaggtgtg ctgaccatgc gtcaagctct ttcatggtca
    27241 agtaacgtcg ggatggtcat actggaacag cgattaggcg gacgatggta taactattta
    27301 caaaaactgg gatttggaca aagtactcat tctggattag atgatgaagt aaacggtgct
    27361 ttacctacat taaatattgt cgaccgagca atgagtgctt atggacaagc ggtaggtgtg
    27421 acgaatttcc aaatgatgaa agctttcact tctattgcga ataacggtac aatgattcaa
    27481 cctcgatata tcagcaaagt ggtggatccc caaactggag aagagcgaac aactcaaaca
    27541 gaagttctag ggcaaccttt ttcaaaagaa acaacagaaa aagtccgtga atatatgaga
    27601 gatgttgtcg aaagcgaaaa ctacggtagt gcttatggtg tatatagtgt acctggatac
    27661 aatgtatcgg caaaaaacgg aacagcccaa atcgcttctg atacaggggg atatcaaaca
    27721 ggagatacag cctacctgta ttcgattgtg gagatggttc cttctgaaga tccagactat
    27781 gtcttgtatc taacaatgaa acaccctaag acatatgatc gaatggcatt ggctaaaatc
    27841 gcaaatccat tgatgaaacg ggcaatggat tttaaagaaa ctgaagaaga ttcagataca
    27901 gaaacaaaaa cagaaaaagt atccgtagcc gattatcgta atcttgaagc ggacgttgca
    27961 gcagctgatg ctcaaaaaag tggactgcaa cctgtagtga tcggtaatgg taaaaaagta
    28021 caaaaacaat ctactgccaa cggtgatcag ttgatctctg gagaaaaact gatcctatat
    28081 actggtggtg ataagctgat gcctgacgta acaggatggt caaaagcaga tatcatgaag
    28141 ctggggaaaa tcctcggtat tgaagttagt tttgatggag atggttattg tgtcaaacaa
    28201 gaacttgctc catatgaaaa aatcactgaa gacaaactta gttttacttt agaagaataa
    28261 gaagagaaat aggagagaaa agagcatgga atggacacaa gcattgattc ccattgtcag
    28321 cagttgtgca atgacaattg ccgcaatgcc gctatttatt ggttattttc aaatgaaaaa
    28381 gcaaggtcag gcgattcgtg aggagggacc aaagtggcac aatagcaaag ccggaactcc
    28441 tacaatgggc ggcttagtgt ttctgatcgg ctctattttg acaggaattt gggttggagc
    28501 ttggcaaaaa cagctaactc ctactctttt catcttgctt tttgtattag cgttatacgg
    28561 agtaatcggc tttcttgatg attttataaa aatattcaag aaaagaaaca tgggactgaa
    28621 ctcgaagcag aaattgattg gacagattct agggggaatc atcttttatc ttgtttaccg
    28681 ttctgaaggt tatccaggtg tattgaattt cttcggacta gatctacctt taggaatcat
    28741 ttatggtatt ttcgctctct tctggcttgt tggattttca aatgcagtaa atctgacaga
    28801 tggcatcgat ggattagttg ctggcttagg aagtatctct tttgcagctt acggtctaat
    28861 tgcttggcat cagcatcagt atgatgtatt aatcatttgc ttaagtgttt taggaggttt
    28921 actgggtttc ttcgtctaca acagaaaacc tgccaagatt tttatgggag atgtcggttc
    28981 attagcgttg ggcggacttt tagcggcaat ttctataatg ctaaaccaag aatggacatt
    29041 gcttttgatt ggtttgatct atgttatgga aacagcaagt gtgatgcttc aagttacttc
    29101 ctttaagctt acaggaaaac gaatttttaa aatgtctcca attcatcatc actttgaaat
    29161 gtgcggatgg tcggagtgga agattgatac agttttttgg ttgatcagca ttgtgacttc
    29221 gttgatcaca ttatggatcg tttggtagga gatgacggga aatgaaaaaa atatcaacat
    29281 atgaaaataa gaaagtgctt gtcttaggac tcgcaaaaag cggagtaagt gcggccaaat
    29341 tattacatga attgggcgca cttgtcaccg ttaatgatgg aaaacctttt gacgaaaatc
    29401 cggaagcaca agagctgctt tctttaggga taaaagtaat aacaggtagt catccaatcg
    29461 aattgttgga tgaagaattt tcattgatgg tcaaaaatcc agggattccc tacagtcatc
    29521 ctttagtagc aaaagcccaa gaaatgggta ttccggtaat tacggaagtt gagctagctt
    29581 acgaagtagc ggaatgccca attattggga ttacaggaac gaacggaaaa acaacgacaa
    29641 caacgatgac aggactacta ctgaatgcag gagcagatca aggaattgca cgtttagcgg
    29701 gtaatattgg gtatccagca agtggtgtag cgcaagaagc aaaatcagaa gataaaattg
    29761 tgatggaatt gtctagcttc caactcatgg gaatcacaga tttccgccct catatagcgg
    29821 tgattaccaa tatctatgaa gcacatattg attatcatgg tacaagaaaa gaatatgtca
    29881 aagctaaatg gaaccttcaa aaaaacatga cagaaaaaga ttatctgatc cttaactgga
    29941 atcagtcaga actacaagaa ttagcgcaaa gaacaaaagc acgcgtactg cctttctcga
    30001 caaaagaagt gttggaagac ggtgtgtatg ctgacgatta ctcaatatac tataaaaaag
    30061 aaaaaataat ggaaatatct gaacttggcg taccaggtaa gcataatgta gaaaatgcac
    30121 tagctgctat ttctgtagca aaattgtatg gtatatcaaa tgaagctata cgagaaacgt
    30181 tgcatttttt ccatggcgta ccacatcgta cgcaatatgt aggagaaatc caaggcagaa
    30241 aattttataa tgattcaaaa gcaaccaata ttttagcgac taaaatggct ctaagtggat
    30301 ttgaaacgtc taaggtcgtg ttattggctg gcggattaga cagaggaaat acatttgatg
    30361 aattaattcc ttcattaaaa ggaatcaaag caatggtcgt atttggccaa acaaaagaaa
    30421 agttgatgga tgctggaaaa aaagcaggca tcgagacaat agtaacagcc gattctgtag
    30481 aacaagcagt tcctttagca ttagaaaaca gtacagatgg tgatgtagtt ttattatcac
    30541 cagcgaatgc cagctgggat caatacccaa attttgaaac tagaggaaac agattcatgg
    30601 aagctgtgaa ccgattaaaa tagaaaaagt agaagcgagc gataaaaaat gaaaattttg
    30661 gttacaggcg gaggaacagg gggacatatt tatccagctc ttgcctttgt caattatgta
    30721 aagactcaag aaccgaatgc tgaattcatg tatgtgggag caaaacgagg tctagaaaac
    30781 aaaatcgtcc ctgatacagg aatgcctttc cataccttgg agattcaagg atttaaacga
    30841 aaaatttcca tgcataacat caagacggtc cgattgtttc ttaagagtat ccgtcaagca
    30901 aaaaaaatcc tcagtgattt tcaaccagat atcgttatcg gtactggagg atatgtttcc
    30961 ggagcggtag tgtacgctgc agccaaaatg ggtattccaa cagttatcca tgaacaaaac
    31021 agtgtaccag gtgttaccaa taaattttta agccgatatg tggatcggat cgctctttca
    31081 tttaaagatg cggcttcctt ttttcctgaa aacaaagcag ttcttgtagg taatcctcga
    31141 gcacaagaag tagcagatac taaaaaatca gatattttga aaacatttga tttggatcca
    31201 gaaaagaaaa cagtcttgat ttttggcggt agtcaaggag cacttaaaat caatcaagca
    31261 gtatcagttt ttttaagtga atatattcct tcttcttacc aagttttgta tgcttcagga
    31321 gatagatatt atgaagagat cctaaataaa ataaatcctg aaatacaaaa tattagtgtc
    31381 cagccttata tcaaaaatat ggcagaggtc atggcttcca gtgatctgct ggtggggcgt
    31441 gctggtgcaa cttcgatcgc agaattcaca gctctggggt taccggcagt gttggttccc
    31501 agcccatatg ttacgaatga tcatcagact aaaaatgcaa tgagtcttgt tcacgcagga
    31561 gcagcaaaaa tgattgcaga taatgaacta acaggtgaat cactaagtca gacagtcaac
    31621 gaaatcatgg gtgatgaaga attgcaaaag caaatgtgcc gagcttctaa agaacaagga
    31681 atccctgatg cttcaaaacg tctttatgac ttagtaaaac aaataatcaa aaaataaagg
    31741 cggtggtctt agtgagtaag aaaaaagaac caaaaaaatt gtctcatccg tcttctgatg
    31801 aaaacaattt gactccttgg caaaaggcta accgaaaata tctggcagaa caagagatga
    31861 aaaaccaaga gccaaaaaaa gaagacataa cattagatac acccctttca gattctgaag
    31921 aagaaaaaac ggttcaagag gaacagcaag aagaaagcca gtccgagaaa aaaatcgaat
    31981 tttttgaaga gatcaagcaa aagaaaaacg gtggaccata taacggttct tttttgaacc
    32041 ggctgcccaa tttaaaaagt caacggcaca aagtactatt tcgtcggtta gctttgatca
    32101 tcactgtatt gggtataccg ctgatttttt tgatctatta tgtttctcct tatagcaaat
    32161 tacaagcagt tgcagtatca ggcaataaga cagtcaacag ccaagaaatc atctctgata
    32221 caaaattgtc attaggagaa aatgtttggg gacagtattt tcatcgatct acttatatcg
    32281 atcgccttaa aaaagcacag ccaagaatcg aaacagcaaa tatacatttc aaagggatga
    32341 atgaattcga tttggatgta acagaatata aagagattgc tttgatcgca aaaaacaatc
    32401 aatactaccc agtgattgaa aatggaacag tattagatga aaaagtagca aatcctacaa
    32461 aaaatctccc tatcttggaa gagttcaaag actctgcaaa aatcaaagaa ttaacaaagc
    32521 aatacaatca gttatcatca gaattacaaa aagcaatttc tgaaatcaag tacacgccaa
    32581 gagcaagcaa taaaaatctg atccaattga atatgaatga cggcaatcaa gttattgtaa
    32641 atatcaataa tcttgcgaac caaatgaaat attattcgca agtagccaaa gatatggatg
    32701 aaaaaggtgt gatcgacatg gaagttggta tcttctcgca tccttacggt agcactcaag
    32761 aaacggaaga atcgggccaa tcggaagaaa caactgaaag tgaagaaaat gcggatcaaa
    32821 caaacgaatc aagtgaagag gcagaaacgc aaaaagcaca aacacctgga tcgtctagca
    32881 caaacggatc atctgattca aatgattcaa gtgaaacaag tcaaatagaa gataatccaa
    32941 gtacaactcc gtcaagttta aatgaaaatt gataaaacaa ctttaaaaag taagaatgta
    33001 ctttctaaaa accagtaatt tatggtagaa ttggaagcag tgaatatatc tattaataag
    33061 tatattttaa aacggacgct agttgttcgc ttgtatatgg aatagtaagt aggagggaat
    33121 cccattcatg gcaaaaacag gaatgtatgt aggccttgat attggaacga catctgttaa
    33181 agttgtcgtg gctgaatata tcgacagcca aatgaatatt attggagtag gaaacgcaaa
    33241 atcagaaggg attaaccgag gcattatcgt tgatatcgat aaaacggtcc aagcgataca
    33301 acgagcagta cgacaagcag aagaaaaagc aggtatccag atcaaaggcg taagtgtcgg
    33361 tttgccagca aacatgcttg aagtagaaaa ttgccaggga atgattgcag ttaacggaga
    33421 ttcaaaggaa atcaccgatg aagatgtcag aaatgtggct tcagctgccc ttgttcgttc
    33481 aatacctcct gaacgtcaaa tcgtgtcaat attgccacaa gattttactg tcgatgggtt
    33541 cgagggaatc aaagatcctc gtggaatgat cggcgtaaga ttagaaatgt acggtctgtt
    33601 gtttacagga cctaaaacga tcatccataa catacgtaaa tgcgtggaaa atgccggatt
    33661 aatcgtcaac gaaatggtga ttactccttt agcgctgact gaatcaattc tttctgacgg
    33721 agaaaaagat tttggtacga tcgtcattga tatgggtggc ggccaaacaa caacggctgt
    33781 tatgcatgat aagcaattga agtttaccaa tcttgaccaa gaaggtggcg aatttgtcac
    33841 taaagatatc tctattgtat taaatacgtc atttaacaat gcagaagcgt tgaaaataaa
    33901 ctacggggat gcatacccag aacggacatc accagatgaa gaatttccag tagatgtgat
    33961 tggtcagtca gaaccagtga aagtcgatga acgctacttg tcagaaatta tttcagctcg
    34021 aatggaacag attttcaaca aagcaaaaga tgcattggac caaatcgaag cattagagtt
    34081 gccaggtggc gtcgtattga caggcggagc tgctagtctt cctggtgttg tggacttggc
    34141 acaagaaatc ttcggcgtaa atgtaaaatt gtatgttcca aatcacatgg ggctgagaaa
    34201 cccggtattt acaaatgtaa taagtatcgt ggattattca gcaaacttga gtgaagtgta
    34261 tcaattggca aaaattgctg ttacgggtga aacctcagca gctcgtcaaa tggtagttga
    34321 acaagaaaca acaaacacat atgagagcta tgaagcacct gaagaaacaa tctacgatga
    34381 gccggaacca aaagagtccg gagaaaatgt aaaaaataaa atcaagggtt tctttacaaa
    34441 tatttttgat taattcatcc tgggaggaat aagatatgga attctcaatt gataataata
    34501 tcaatgacgg cgcagtaatc aaagtcatcg gagtcggtgg tggcggtgga aatgctgtaa
    34561 accgaatgat tgaagaaaac gttaaaggtg ttgaattcat tacagccaac acagacgtgc
    34621 aagcattaaa aaattcaaaa gctgaaacag ttatccaatt aggccccaaa tatactcgtg
    34681 gtttaggtgc tggttctcag ccagaagttg gacaaaaagc ggcagaagaa agtgaacaat
    34741 ccttacgcga agctttagac ggtgcagata tgattttcat cactgcagga atgggtggag
    34801 gaaccggtac aggtgctgca ccaatcgttg caggtatcgc gaaagaattg ggcgcattga
    34861 ctgttggtgt cgtgacacgt ccattcactt ttgaaggacc gaaacgtggc cgttttgctg
    34921 cagaaggtat cgctcgtttg aaagaaaatg tggatacatt attaattatc tcaaataacc
    34981 gtttattaga agttgtagat aaaaagacac cgatgcttga agctttccgt gaagcagata
    35041 atgttttacg acaaggtgta caaggtatct ctgatttgat cacagcgcca ggttacgtta
    35101 accttgactt tgctgacgtg aaaacagtga tggaaaacca aggtacagca ttgatgggga
    35161 tcggtgtggc tagtggagaa gatcgcgtag tagaagcaac gaaaaaagca atctcctctc
    35221 cattgcttga aacatctatt gatggagctg aacaagtact tcttaacatc acaggcggtt
    35281 tggatatgac attgttcgaa gcacaagatg cttcagatat cgtagcaaat gcagcaactg
    35341 gcgatgtaaa tatcattcta ggtacatcta tcaatgaaga aatgggcgat gaaatccgtg
    35401 tgacggtcat cgcaacaggt atcgatgaat caaaaaagga acgcaagtct agccgaccag
    35461 ctagacaagc acaaatgcaa tcacctgctc aaaagactgt attagatatg gatcaagcta
    35521 aaccaacttc ttcagaagaa gaaaacagtt ttggcgattg ggatatcaga cgtgaacaaa
    35581 atgttcgtcc tagagttgat gattccaatt ttgacaatat cgaaaaaaaa gaatttgata
    35641 cattcaaccg cgaagaaaca aaatctcatg gcgatgatga attaaatacg ccaccgtttt
    35701 tccgtcgtaa aaaataaagg agaggaagag cacatgattg ctgacaactt gcagaaaatc
    35761 aagcaagaaa ttcaggattc gtgtgctctt gttcatcgtg atccgaaaga agttacatta
    35821 gtagctgtaa caaaatctgt tgaatccgct caagctgaag aattggttag agctggcgtt
    35881 atgaattgtg cagaaaaccg agtagataaa ttattagaaa agaaacaaga tttatttgat
    35941 tttcctgaaa taaagtggca tttaattggt aatttacagc gcagaaaggt aaaattgatt
    36001 gtaaatgaaa ttgattactt tcatgcgttg gacagtttaa gattagcaga agaaatccag
    36061 aagcggttag agaaagaatt atcctgtttt attgaagtaa atgtttctgg tgaggaatca
    36121 aaacatggaa tttcaccaga tgagctttta ccgtttgtgg aatctttagc tgcctgcaca
    36181 aaaatcaagg ttgttgggtt gatgactatg gcgcctaaag aagcttctga tgagcaaatc
    36241 cataaagtgt ttggtacgct gaaagaattg cgtgatcttg ttcaagcaaa aggttatgcc
    36301 catgcaccat gtagtgaatt aagtatgggg atgagtcaag actttcaaat tgcgattgaa
    36361 gaaggtgcaa catttatccg tgtaggaaca gcgctattca aagacgaata aaggaggaac
    36421 aaaaatgccg cttttaaaca gaggagcaat tgctaatttt ttcggattag ctgatgagga
    36481 agagtacgaa taccaagatt atcctgaaac aacaaaaaag gctgtacagc caactaaaaa
    36541 aaatacaaca cagaatcaat caactcattc tgtatctgct gcgaatactc atgaaaaaca
    36601 aaaaacggcg acttcacgtc ctgtaacgca tgaaagtatt cagccggctg aaaagaaaaa
    36661 agtaattgaa ctacatcaat cacctacaaa aactgtagcg aaagaaagaa cagcttcaag
    36721 aacctctaca caacctaaag caaatcttgc tggaaaaatc actgtaatgg aaccaagagc
    36781 ttattctgaa gctatggcga ttgctaaacg aatcattgct ggagaagcaa ccttagtgaa
    36841 ttttcattta gtagaagaaa aacaagcgag aagaatcgtt gattttctaa caggaacagt
    36901 ttatgcacta gatggagata tcaaacgtgt tggagatgag atttttcttt gtacaccgac
    36961 tgggatggag attgacagtt caaccgctca atcttttgca gatgcgaatt tgtttgatct
    37021 gtaagaggag gaactacgat ttatactatt gcactattac ttcatttact tcaacgggca
    37081 gcctatttct atagcattat cttgatggta tatgcattgc tctcttggtt tccaggcggg
    37141 tatcaatcag ggctaggacg attattagct aagatctgcg aaccgtatat cagtttgttc
    37201 gatcgtctgc ctttgaggat tggacccttg gatttcacaa ttttaggggc aatcctgatc
    37261 ttgaacttag ccgtacaagg tttagcacag atcgttatat ggataactgt ccatttagtt
    37321 tattgattga agcaacaaaa tctttattct cagaatagat aggaggggaa aattttggat
    37381 gcaaatgtat accagcactt tcgggcagat gaacgtacat ttatcgattc ggtaggcgat
    37441 tggattgaac aggttcaagg acaatatgca ccctatctga ccgaatttct tgacccaaga
    37501 caatcctata ttttagaaac actgattcga caaagcagtg atttgcgttt catgttttat
    37561 ggcggatatg aacaggcaga aagaaaaaga tgtttgattt tccccgatta ttacgaacct
    37621 gtagaggaag attttgaaac agcattgtat gaaatccatt atcctagcaa atttgccagc
    37681 ctaagtcatg ggaaaatcct tggaacttta gtgggaacgg ggatcaaacg ctcttacttt
    37741 ggcgatatca tctcggacgg tgatcgctgg caagtattta ttgcaaatga ggtggatcat
    37801 tttgttgctg cacaagtaac aaagattgga aaggttgctg tacgcttgga accaatcaaa
    37861 tacacagaaa tgattatgcc gaaagatagc tggacacaag aaagaggaac agttagttcc
    37921 atgcgactag acagtgtgat ctctgagatt tataatattt ccagacagcg gtccaagcag
    37981 ctgatcgaag ctggtaaagt aaaagtgaac tggacagaaa atacaagacc tgattttgta
    38041 cttgaactat tagatattat ttcagtcaga ggttatggca gagtccagat ccaagaactt
    38101 gaggggaaga cgaaaaaaga aaagtacagg gtactgttcg gtgtattacg taaataaaat
    38161 aaaaaggtgg tttactcatg gctttaactc cattagatat ccaaaataag acattcccga
    38221 caaaaatgcg gggatataat caagacgaag tagatgattt cttagattta gttgttcgtg
    38281 actatgagga attgactcaa agaaatcgag aattagaaaa agcagtcaaa cattctgaag
    38341 aaaaacttga gtacttcaat gaattaaaag atgctttgaa ccaatcaatc atcgtagctc
    38401 aagatacggc tgataaagta aaaacaagtg caagcaagga atccgaagtg atcgttactt
    38461 ctgcacaaaa caaagcagat gagttagttg cgaacgctga aaaacgtgca catcaattaa
    38521 caactgatgc agaagaaaaa gcaagaaaaa tcttgacaga tgcaacagaa aaagcaagac
    38581 agcttgcgac agaaactgaa gatcttaaga aaaaaacacg agtattccat caacgtatca
    38641 gcttgatgct tgagtctcaa ttagaacaag tgaaaagtcc ggaatgggat gaaattttac
    38701 aaccattctc cagctacgtg actgacagcc atgaagtgat caaagaagtt ttagcaaaac
    38761 agcttgacaa tgaaaatgat actgatgtaa actcagacac aggagtcata gaagcaccta
    38821 tgacggcatt tgatacacag gctattgata tctcgatcgt gcctggtgaa aatgaaaacg
    38881 agtagaacag aaaagcactt tgttattctt aaagcgagcc gatgagagtg gaagttcggt
    38941 aagacactgc atggtgaaag atcctctact tgagacctgc ttttccgaaa ttagagtaag
    39001 aagcagcggc tgacctcgtt attggtcaca gagagtttgc actagataga tgcaactaaa
    39061 tttgggtggt accacggcat gttcgtccct tgcggctgaa catgcttttt tgtgtccgga
    39121 aaacaaataa aggagtgtat ggttgaatga aaatgaaaga aaccttgcaa ttaggaaaaa
    39181 caaaattccc tatgcgtggt aatttgccaa accgagaagg cgagtggcaa aaagaatggg
    39241 aagaaaatcg aatctaccaa aaaagacaag agttgaacga aggaaaacct agctttgttc
    39301 ttcatgatgg acctccatat gccaatggta atatccattt aggacattca ttgaataaaa
    39361 tcagtaaaga cattatcatt cgttcaaaat caatgtctgg ctatcgtgca ccatatgttc
    39421 caggatggga tacacatgga ttgccgattg aacaagtatt ggctaataaa ggcgtgaaac
    39481 gtaaagaaat gtccatggca gaatacttgg aaaaatgtcg tgaatatgca ctttcccaag
    39541 tagataagca acgagcagat ttcaaacgcc taggagttgc aggagattgg gaacatcctt
    39601 atgttacatt agatccttct tatgaagctg cacaagtacg cgtatttggt aaaatggctg
    39661 aaaaaggcta tatttacaaa ggattgaaac caatttactg gtctccgtca agtgagtctt
    39721 ctttagcgga agctgaaatt gaatataaag atgtaaaatc tccatctatt tttgtagctt
    39781 tcaatgtcaa agacggcaaa gatattcttg atgaagatac agcttttgtc atttggacga
    39841 caacaccatg gacattgcca gcaaaccaag gtattgctgt aaatccaaaa tttacgtatg
    39901 tattggtgga agccgatggc cgtaagtttg tcgtagcgaa agatttgcta gaaacagtcc
    39961 aatcagaaat cggttgggaa aatgtaacag ttctaaaaga atttgctgga caagaaatgg
    40021 aatatatgac tgcaagtcat ccattctatg aacgtgaatc acttgtgatt ttaggcgatc
    40081 atgttacttt agacgctggt actggattag tccatactgc tccaggacat ggggaagatg
    40141 actatatcgc tggtagcaaa tataaactat ctgtagtgtc acctgtagac tcaaaaggat
    40201 tgtttacaga agaagcacct ggatttgaag gtatctttta tgacaaagca aatcctatga
    40261 tcactgatct attaaaagaa aaaggagctt tgctgaagct agatttcttt actcatagtt
    40321 atcctcacga ttggcggacg aaaaagcctg tgatctatcg ggcaactcca caatggtttg
    40381 catctatcga taaattccgt cagaatattt tggatgaagt agaaaaagta gagtgggtta
    40441 tcccatgggg taaaacacgt ttgtataata tgatccgtga ccgcggcgat tgggtaattt
    40501 cccgtcagcg tgcatgggga gtaccacttc cgattttcta tgctgaaaat ggagaagcaa
    40561 tcatcacacc agaaacaatc gaacatgtgg caaaattatt tgctgaacat ggatcaatta
    40621 tctggtttga aaaagaagcg aaagaacttc ttcctgaagg atttacccac ccaggttctc
    40681 ctaatggcga atttacaaaa gaaaaagata tcatggatgt ctggtttgat tctggttctt
    40741 ctcatgaagc tgttttacgt cagcgaccag aattatcatt cccagcagat atgtatttag
    40801 aaggatcaga ccaatatcgt ggctggttca actcaagtat cacgactagt gtggctatca
    40861 atggtgtagc accatacaaa tcagttattt ctcaaggatt tgctttagat ggtgaaggcc
    40921 gtaagatgag taaatcttta ggaaacatca ttgcaccaga aaaagtgatc aaacaatttg
    40981 gtgcagatat ccttcgctta tgggtatcta gtgtagatta tgaagcagat gttcgtgttt
    41041 ctatggatat cttaagtcaa gtggcagaag tatatcgtaa aatcagaaat acgatgcgtt
    41101 tcttattagc gaatacagaa gatttcgatc ctaaaaaaga tactgtttcg tacaatgatt
    41161 tacgttctgt agacaaatat atgaccgttc gattgaacca agtaatcaaa gaaatccgag
    41221 aagaagggta cgacaaatat aacttcatgc atatttaccg tacagttatg aacttcttga
    41281 cggttgatct atcgtcattc tacctggatt ttgctaaaga tgtagtttat attgaagcag
    41341 aagatgatta tcaacgtcgt tgcatgcaaa ctgttttcta tcaaacagct gtagctttga
    41401 caaaattatt gactcctatc attccgcata cagcagaaga aatctggtca ttcctgaaag
    41461 aagaagagga gtacgttcag ttaagcgagt ttcctggtta tgaagagttt gcaaaccaag
    41521 aagaattgat ggatacctgg actgctttca tggacttccg tgataatgtc ttaaaagcat
    41581 tggaagaagc aagaaacagc aaattgattg gtaaatcttt ggaagcaaaa ctaactgttt
    41641 atccaaaaca acaagttcgt gaaatgttaa aagctttgga tgcagatatt gcacaattat
    41701 tgatcgtttc accagactac tttgaagtta tggcagcaga tgaacaagtg ccagataacg
    41761 ctatggtatt tgatgatgtt gctattacag tggaaaaagc agacggggaa acttgcgatc
    41821 gctgtcgcca agttcgtaaa gatgtaggag tagatgaaaa acttcctcat ctatgcggtc
    41881 gttgtgcaaa aatcgttgaa gacttctatc ctgaagctgt agctgaagga ttcgaagaaa
    41941 agtaaaaggg aaaagggggt gtgacatgtt ctttgtcaca cccccttttg ttatcaatcg
    42001 tttaattttc cacgttcacg ataccagtta tctggttgcc agatccattc gaatccgtct
    42061 ttttcaagta gttcgaaagc tgctttaggt cccattgatc cagctttgta gttgggaaaa
    42121 tccggttgtg tttggtccca agcttttcga atgacatcca cgatatgcca agattgagcg
    42181 acttcttccc aatgagagaa gttcgttcca tcaccattca acgcatctaa tagtaatttt
    42241 tcgtaagctt ctgggctatt ttcaaccatt tctgcactat ttctaaattc caatttgatt
    42301 ggttccgttt caaatccttg ccctacttct ttcccattca aggataaaga aaatccttca
    42361 gttggctgaa tatagatcgt tagaacatta ggtggaagtg tcgtatcgtc acatggttca
    42421 tcgacagatg ttttgaatac attaacagga acttgtttga atacgatgtt gatccgagtc
    42481 cctttttcag tcaaacgttt gcctgtacgt acatagaaag gaacacctga ccaacggaag
    42541 ttatcgatag tgaatttgcc tgcgacgaat gtttcagtct ctgattgagc atccactttg
    42601 tcttcttcgc gatagccctt gaattgtaca ccatccaatt caccttgagc atattgcccg
    42661 cgaacgaagt tttctaacac ttcttcttcg ttataaatgc ggatagcttt caatgctttg
    42721 atcttttctg tacggatttc tttttcggaa aatgctactg gcggttccat tgcaagtaat
    42781 gctacaacct gtaaaatatg attttgcacc atgtctttaa gtgcgccact atgatcgtag
    42841 tagcccccgc gatcttctac accgaggttt tcgccaaaag taatttggat attgtcgata
    42901 tagcgattgt tccattgagc ctcaaaaata ttattggcaa aacggatagc tgagatgttt
    42961 tggatcattt cttttcctaa ataatgatca atcctgaaaa tatcttgttc tgggaatact
    43021 tctctgattt gctggttcaa ttcaaaagca gactggtaat cagaaccgaa cggtttttct
    43081 atgatcaaac gattgaagcc attttttgtc ataatatttt gtgatttcaa atgagaaacg
    43141 atcgttccaa aaaattgagg tgccatagcc aaataataga cacggttacc gtttaattga
    43201 tagtgtccat ctaatcggtc agacaattct tttaatgtct gataatgcgt tgaatcattt
    43261 acatcatggg attgatagta aaaatggctt gcaaaggtag ttgcttcttc atctgaagga
    43321 ttcaatgcac ggattgtttc tttgacaact tcacggtagt gctcatcgct ccagggacgg
    43381 cgagcggttc cgattacagc aaaatgctcg cctaaatttc cttttctgta aaggcggaac
    43441 aaagacgggt acaatttacg ctgggctagg tcacctgtac cgccgaatat cgtaaacaat
    43501 acattttttt cattagacat gtggatacac tcctttatat tttatcgaca aaaatcgtgc
    43561 tggccgcctt aaagctgacg gggattttct ttctatcatt ttggatagtg atcggtcctt
    43621 cataagctgc gatgtcaacg atctcatatt cttcgttgat attcaggtca atagtgacta
    43681 agtaatccaa caattctttt tcatcaagca ctcgtgcgat acgcactttt gtaccgacag
    43741 gataggactc caatgtttgt cggcgttctt catggacaac ttcctctttt ttaggaatcg
    43801 atcctccatg aggacaatgt tcaggatgat tcaaatagtt ttctaaatga tctgcaagat
    43861 gttccgaagt cacatgttcc aatacttcag catcatcatg gacttcattc cagctatagt
    43921 ctaaatgttc aactaaaaaa acttcccata agcgatgttt gcgaatcaga ctgctagctt
    43981 tttgcagtcc gcttgcagtc aattgtacac cttgataagg ggaatgttct acaagctctt
    44041 ctttcacaag tttagaaatc atttcactga cagatgcagc tgagacatct agactagata
    44101 cgatttgttt attgttgact ttcgttgtat ctccacctag ttctaaaatg attttcaaat
    44161 aatcttctcg atttggtgtc atattcgatc atcctttcat ggtaaaaaac catcattagt
    44221 aagtgtaaca aaaaaaacgt taattgacta tttttctaaa taaaaaacaa aggattttta
    44281 gacgttttta caacaaaaaa cgaaagatga tagagaagac tcattctttc gtttataaag
    44341 gatatatata tacattggga gggatctatt tttcagataa caacttatat gaagtgtctg
    44401 cagattcgtc ataaagagtt tgttcgggat aagaaaagat aagataaaaa ccatactgat
    44461 cacgtacgac taaacgatat tcatctattt ctaccacaga tgtgccaaga gactgaccgt
    44521 tgatcaaaaa cgtgtaatca gatgtaatct gtaattgttg tttttgctgg ctatcactaa
    44581 agaaccatgt accttgatac tttgagagtc cttctgactt ttccgcttgt tttttctttt
    44641 tcaagtaaat aaaacttgag agaacggtaa tgagtgaacc aagaaaatag atcatttttt
    44701 ttatcaccaa atcactcctt tatgagaaaa taatcgcata gacatattct ttcttcttta
    44761 tttcattgta ccgaataaaa gctaaaattt ctctaagact ttcgactggt ttttttactg
    44821 tttttctaca aattatgata ggatacatag taggaagaaa ggtgacataa tttgaagtat
    44881 cagcaagtgg aaatagcttt ttttattgac cgcccgaatc ggtttatagc acattgtatg
    44941 aaaaaagacg gagaaataat tattgcgcat gtcaaaaata caggtagagg aaaagaggtc
    45001 ctgataccag gcgctgaagt ggctattgta tttgctcctg gacctaaacg gaaaaccgcc
    45061 tatgatctga ttgctgtaaa aaagaagggt gactggttta atatcgatag tcaattgcct
    45121 aatcggctgg caattgatgg tattttagac ggcacgatcc ttttaccagg attaactggt
    45181 ccatttacgg tatacaagcg tgaagtaact tatcttcact ccaaatttga tatttattta
    45241 gagacggtgg tcggacaaaa agcatttgtt gaagtcaaag gtatgacact tgaaaataaa
    45301 gcgattggtg cttttcctga tgcgcccaca ctcagaggat tgaaacatat aggagaatta
    45361 acgtatgccg cacaagatgg ttatgccgct tatgtcttgt ttattgctca gttcgaacat
    45421 cttcatctgg caacgatcca tgaagaaatg caaccagcac tagctgatat ggttcgacat
    45481 gcgcagcaaa gcggagttca aatcctcgcc tataattgtc aagtatcggc agatgaggta
    45541 actgtcaagc aggccattcc atttgatgta gatatccctt ttgaagatcc tatacaatag
    45601 aaagaaggta aaataatgat caacttaggt attatcggaa caaattggat tactcatcaa
    45661 tttgtccaag cagctttaag cacaaaaaaa tatgaattga cagcggtata ttctagacat
    45721 ttggaaaaag cacaggaatt tggttctcat tatgaagggg atatcgaatt cgcaacagat
    45781 ttgaagactt ttttcaatat tactcatatg gacaccattt atattgcttc tccgaatagt
    45841 cttcacttta gtcaagctaa gcaagcaatt ttggcaggaa aaaatgtgat agttgaaaag
    45901 ccagcttttt cgacgccaaa agaaatggat gaaatcatcc atttggcaaa tgaacacaat
    45961 gtgtattttt ttgaagcagc aagaaatatc catgaatcag gttttaaaaa gattgccgat
    46021 tacttaccat taaaagacga gataatcggt gcaaatttca gctatatgaa atattcttcg
    46081 cgctatgacc aagtattaga tggagaagaa ccgaatatct tctcgactca tttctcaggt
    46141 ggtgcattga tggatttagg tgtttacctt gtatatgcag ctgttgcttg gtttggaatg
    46201 ccaaaagaag tccattattt cccaagaaag atttctactg gtgtagatgg tcttggatgg
    46261 gggatcttac gttatgagaa ttttgatgta tcgatccagc caggtaaaat cggtgattct
    46321 tatttaccgt cagagattta ttttgactca ggaacgctag tgatgaatgg agtcaatgca
    46381 attgaacaag cacaatttca tgatcgcgcg cataaagaaa tcattgattt ggcggtaaca
    46441 gctgcagaaa atccaatggc agatgaagca gaggattttg ccaatgtcat ggctcatccg
    46501 aaagatccag cttggggatt gttgtatgaa gagtggatcg agctagctag aaatgtgaac
    46561 caagtgattt atgatttaag aaaaaatggc ggcatcaaat tcgatgcaga taatgaggaa
    46621 gatttttaat gaacgaattt gtacaagtat ttcccgaaaa ttggcagaaa aaatatgagg
    46681 ataaagggtt tatctcacct tctcttattc aacgagcagt ttttcagcca ctaagtaata
    46741 aacaaagtat agtagcggtt tctcctacag gttcaggtaa aacactggct tatctttggc
    46801 ctctactact aaacgtggaa ccaggtgaag caagtgcgtt ggtcattttt gcttcttctc
    46861 aagaactagc tattcaagta gcagatgttg cacgtgaatg gggaaaagac aaagagttga
    46921 aagtccaatc tttggtgggc ggagccaacg tcaaacgtca gattgagggt ttgaaggaaa
    46981 aaccagaaat cttgattggt acaccaggaa gaatattgga attgatgaaa gcaaaaaaaa
    47041 taaaagcaca tcaagtgaaa acaatggtat ttgatgaagc agaccagcta tttgacgctg
    47101 ggaacagcca aatcattgat caaattcttc atcaagctcc aacagagtat cagctagctt
    47161 ttttttcagc gacagcagat cgttcccttg aaagcattga aaaaatcact gggaaaacgt
    47221 ttgagacaat tgacgtgaca gcagaggacg attctcgaaa aggactgaga cattactttt
    47281 tacgtgtacc gatacgaaag aaagatgact acttgagacg tctcacgcat atagaagatt
    47341 ttcatggtct gattttcttt aatcaactga ctgaattagg cgtaatggaa gaaaaattgc
    47401 tgtatagagg tgtaccggta ggaagtctgg cctctgatca gaacaagcta ctccgaaaag
    47461 cagcgctaga acaattcaaa aagaaaatgg tcagagcact tttgaccaca gatgtggcgg
    47521 ctagaggttt agatattaca ggtcttcctt atgtagtgaa tgcagaagtt ccgttaagtg
    47581 aagaagctta tttgcatcgt tcgggaagaa ctggtcgaat gggtaatgaa ggaatagtaa
    47641 ttacactcgt acaagataat aatctaagag atttaaaacg gcttttacgt ccatcaaata
    47701 taacacttga agagattttt ttatacggag gtaagctgca aactgagcag ccaaccaaag
    47761 aagatgcagc tcctgcagta aataaaaagt atgcgaaaac tttttctgga aaggagcaga
    47821 acccagaata taacaaagaa aaaataagaa aaaaagttaa aaataaatca aaaaaagaga
    47881 gaaataaagg agcaaggcgc aaataagcgc ctttttatgc tatgattatg aaaacgggaa
    47941 caaaattggt tgccagagga ggggttatgt gaaagaacgt caagaagaga tcttgcagct
    48001 attgacagaa aaccaacaag gattgacagc aagtgatgta gctgagcgct taactatcga
    48061 tcgaagcaat gcaagccgat atttaagtga actatacaag gcccatcata ttgtaaaaac
    48121 cgctggccga ccagtcgttt actctttgcc aacggaaaag tcaaaatctg atgaagtgca
    48181 tgtcgactct tctacgcaag tgacttttga aacactcgtt ggagaaaatg attctttaaa
    48241 agtcagcata cagcaggcaa aagctgcgat tctttatcca ccgcgggggt tgcatacgat
    48301 tatttttgga gaaactggta cagggaagtc gatgtttgct gaatgtatgt atcattttgc
    48361 aatcgattca gagatgcttt ctgcagatgc gccatttgtt tctttcaatt gtgcggatta
    48421 tgctcaaaat ccgcaactgc tctttggaca catctttggg atcaaaaaag gggcttacac
    48481 aggagcagct caagatagtc ccggtctaat agccaaagca gacggcggta tattgttttt
    48541 agatgagatc caccgtttac cgccggaagg gcaggaaatg ctgtttactt tcattgataa
    48601 aggtatttac cgtccgcttg gagaaagtgc acaagtacat gaagcatctg ttcagatcat
    48661 tggggcgacg actgaatctt cagaaagttt cttatctacg ttcaatcgac ggattcctat
    48721 ggcaatcacc ttgccgaatc ttgcttcacg ttcactggat gagcgttatg aaatcatctc
    48781 tctttttatc aaacaagaag cgaatcgttt gaatcaacgt atcgatatcg aaaaagaagc
    48841 gattcttgcc ttcatgcttt atgatgcgga aggaaatatc ggtcaagtga aacgagactt
    48901 gaaactagtc tgtgcaaaat cttttttaca ttaccgtact catcatgaga aaaagctaat
    48961 cattcgaaaa gaagaacttt cccttcaagt acaaaaagga ttactgaaaa tcaaggaaat
    49021 accggaacgt ctcgaccgat tcatggacag caaaagtcag tatttgacat ttgaaccagg
    49081 atttgctgat gttgtctggt cacaagatcc agaacgaaac atgcaggtct ataacgatat
    49141 tgaggaaaaa gtcacttctc tttccgaaac cggtgtggag aacattgatc tagagaccct
    49201 catttcaaaa gatgtggatg cttatttcca gacatatgtc aaagaattga ctaaaagttc
    49261 gattcccaaa gatcttttgc cagatgaaat ctggcggtta accaatcaat tgtatgatgt
    49321 ggctgaaaaa cggttagacc gtatttataa tgaaaaagct cgttttgctt ttgctttgca
    49381 tctgcaaagt acattggatc gtgtgagaga aggtcatatg atcgtccacc cagatttgaa
    49441 taacgtacgg aaaaatttta agagggaatt ccaggtagca atcgatcttt caacgatcat
    49501 tgaagaagaa gaacaagttg agattccttt tgatgaaatc ggctttatta gtatgtttct
    49561 ttctatcaat ctaggagaaa gtgagcctgt taaagaaaac aaagtagatg ttctcatatt
    49621 gatgcacggc agacagactg cttcaagcat gttggagatg gcacaagaac tattaggaac
    49681 aaaagtaggg aaagctttta atatgccact ggttatggaa gttcaaacaa tgtatgaaga
    49741 agtgttacaa tatgtcaaaa actatcaaga gcatttgacg aatggcctac tcttgttaac
    49801 agatatggga tcattaaaca ccttcagtga tttgatttat gaagagactg gcattcggac
    49861 gaaagctatc acgatgacaa gtaccatgat tgtgatagaa gcgatacgga tggcaagtgt
    49921 tggaagaagc ttagaagata tttatcaaaa tatccagctt tcctttgaat cgatcgtacg
    49981 tgatcagttc aaagcggaaa agtctgaaac tttacagcgt aaaaaagccg taatcgtgac
    50041 atgctttact ggtgaaggag tagctgccaa attatatcag cgtattatgc cagtgattaa
    50101 tcaaaataag gtagaactga tccagatgca gtttttagaa cgtgaatcat tcaaaaaaca
    50161 catcgatgcc ttattggaag aatatgagat caaagcaatt gcgggaacag ttgatgttga
    50221 ttatcaaaat atcccatttt tttcagccta tgacgttttt gatgatgaaa aactaaatat
    50281 tttaaagcgt atagctgatg atgagatccc gacagctaaa attgtcgatt cacttgctgg
    50341 atcattaagc gatgtctttt ctattgatga actgatccgt tcattgcaaa agacagttca
    50401 gcagatccag accgatttgt cgattatcgt ggaacctgga gtagatactg gaatcgttat
    50461 ccatttagcc ttcatgttag acggattgat caaacaagaa agccatcggc aatttgagaa
    50521 actgtctatt ttctcaaaaa tttatcgttt ggaaatggat atcgttcggg caaacttgat
    50581 ggcactagaa cgaaaatatc aggtaaaaat tccagaagat gatatcgcat ttcttacaca
    50641 aatgtttctt gaaaataaaa taaaaaaatg aacacactaa tcatactgtt tacacactaa
    50701 acatctgtgt gcgcactata aaaaagagct aaaaagcatg aaaacgctat ttcagctctt
    50761 ttttttattg gcatgcttct tgcgttatat aaaagtgtaa aaaaataaaa ggagtgttaa
    50821 aaatggcaaa aaaaacaatc atgttagttt gttcagcagg gatgagtacg agtttattgg
    50881 tcacaaaaat gcaaaaagca gcagaagaaa aagggatgga agccgatatt tttgcggtct
    50941 ctgcctcaga tgcagataat aatttagaat ctaagaatgt cgatgtatta ttattaggtc
    51001 ctcaagtacg tttcatgaaa gctcaatttg aacaaaaact tgcgcctaaa ggtattccat
    51061 tagatgtcat caatatgcaa gactatggca tgatgaacgg agaaaaagtt cttgcacaag
    51121 cagaaaatct aatgaaataa atcaagtatg cagttggggg tataacaacg tggaagatca
    51181 acaaaatcta gaagccatca tggggttaat catgtttggc ggtaatgcaa agagcgatgc
    51241 aatggaagcg attgcggctg ctaaaatcgg tgattttgag ttagcggata ggaaaatcgc
    51301 tgatgcagaa gaatcactta tacaagcgca tcatgcacaa acagacatgt tgacacaaga
    51361 agcacaagga aatcatatgc aagtgacatt attgactgtt cacagtcaag atcacttaat
    51421 gacatctatt gcgtttactg atttagcaaa agaaatcatc gaactttatc gtcgcgtata
    51481 cagcgaataa atacaacttc ttttttgaac ggaagaagaa aaatttttga acggattttt
    51541 tattttgtta taaggagaac tctctttata taaaaaactt ataggaggaa gtaaaaaatg
    51601 aacggactat caagttggat ggagaaatac atcctgccgg ttgccgcaaa aatcggagcg
    51661 caaaaacatt tagttgcttt gcgtgatgct tttatcggca cactgcccgc aacaatggcg
    51721 ggatcagtcg ctgtaatgat caatgcaatt attcgtgatt taccgcaaca gtttaacagt
    51781 acctatgctg acagtttagc agcagacaaa ggaatctttg ccgtattgaa tgtcatcatc
    51841 ggaatcaatg gttttgtctg gaacggtaca ctagcaatcg ccggattgat ctttgccttt
    51901 tcttgggggt ataatcttgc ccgagcttat ggagtaaatg atctagctgg ggggatcgtt
    51961 ggtttagcta ccttgattca aggtatttcc tttggtccag cattatcagc agcattagat
    52021 gtgaatgttc cttcaaatgt cgcagatgca atcaatagtt ctgagattgg tgcaacgatt
    52081 gcagatggca cactgtcagt tggattatgg ggttggctaa aactagatca tttgaatgga
    52141 aacgcttatt ttacagttat gatcatggga gctatttcag tcattatttt ttgtaaattg
    52201 atgcttgcca acattacgat caaaatgcct gattcagtac caccagcagt atctaaagca
    52261 tttgcagcca ttttaccagc gacgatcgct ttatacgtca ttgctattat caattatatc
    52321 gtaggtcgtg tagctgatgg gcaactgatc attgatttgg tgcaaaaata tattgcagag
    52381 ccattcctag gattatctca agggttggga gcagtattga tcgtcacagt atttgttcaa
    52441 gtattctggt tctttggtat tcatggacca aatgtattgg caccagtgct agagggaatc
    52501 tggggacaag cacaactaat caatattgat attttccaaa aaggttataa cggaaaaact
    52561 ggtacaccag ccgtgttaga tgcaatcgaa gatggaaaag cttatatgtg ggtccgcgga
    52621 tcgttcgatg cttttgcatg gtttggcgga tcaggcggaa cgatcgtctt actgatagct
    52681 atcttgattt tctctaaacg tgcagattat ctaacagtag ctaaattatc cattgggcct
    52741 ggtatcttca atatcaacga accagtgatg tttggtctgc caatcgtatt gaatgcaatc
    52801 atgtttatcc cattcttgat tgctccagta gtagcaacag caattggatg gacagctact
    52861 tatcttggcc tagtcgctcc ggtatcgcag caagtcacat gggtagtacc gccgttctta
    52921 ttgtcattct tagcaacagg agcggattgg cgggcaccga tcgtaacatt agtatgtatg
    52981 gtcgtgactt tcttgatttg ggcaccattt gtcatctctg caaacaaaat ggaccctgcc
    53041 gcaggcgaac aacaataaga tgaacgtgag gagcgggacg ctttaagttt cgttcctcat
    53101 aatgaatttt cgaggaggaa ctgaaatgat tacaattgcc agtgcaatgc aacaagatga
    53161 aatcaaagaa ttgttagaag gatacaatga agaaggaatg acttttacat ttaaagaaaa
    53221 acaaggaatc aagctgttct ttgaaacaag tgcagaagat ccggaagttg cggctaaaaa
    53281 agcaaaggaa ctaatcaaag cacagccttg gggaacagtc ctttattttc aatcaacagc
    53341 tgtttagaaa atggtttaaa aagataggat ggttgagatg ataaaaaacg gtttattttt
    53401 atgtagtatt ggttctttga tcgtgttgta tagtttgaat ccacattcgc aaaggtatga
    53461 tttgtatagt atggtcacag gtctttttct aacaggatta ggaatctatt tcttcatgaa
    53521 ggggaaaaaa agagaagaag caaaggagaa aaataactaa tggaaatcac gaaaaaatta
    53581 aatattccag catcgttttt ttacgataaa gttatggact ctgtcctttt cgatgtacgt
    53641 aaagcgaccg gcaaatcagt aactagaaaa cagctgaaaa atttggagta cgtcaaacaa
    53701 ttctcgcaaa ataaccgggc tcgcataaaa atcgaagaag tcattgaaaa ccagtcttat
    53761 aagtttcgta catcgacaac gaagaatgaa ttcgtcgttc attatcaaat caaaccattg
    53821 gacgattcga cctgtgaggt tcattacaca gaaaaaatgg agtcttatgg catgcttcaa
    53881 aagttgaatg atatgctttt aggaacaatc ttgatgtatt ttaaacggag acgtttcaaa
    53941 aaaatgctag atatgatcga agcaacgtat taaatcaatg aaacatggta ctatttagtt
    54001 attgaaaaat gaagtaagct agaaggaatt tttcatagga agatagatct ttcctgcgaa
    54061 tcctcactag cttttttgta ggaaggggca gcaaaatgac aaacaaagta agagagctag
    54121 gtgtatcttc gattgaggaa atgtttgatt tagccactta tgcatttaat ggggcagata
    54181 cacctgaaag gcgagaacgt tttcgtacac ttgcggagaa ttcttggaat tacggatttt
    54241 ttgatgagga ggatcggcta acaagtcaag tcatggcgac cccttttcca gtgaattttt
    54301 acggacaaga gtacctaatg gccggcattg gctatgttgc ttcttatcca gaagcaaggg
    54361 gacaaggcgg gatcaatcag atcatggaaa agatccttga agattgccgt gatcgtaaag
    54421 taagcttatc ttatctggcg ccattctcct atcccttcta tcggcgctat ggctatgaac
    54481 aatcatttga taaaatcagt tatcaactaa acagtcgtga tgtgccgttt atcaaaaaaa
    54541 aatctggaaa aataaagcgt atggattttg aagatgcaaa agctgcaatc agaagaattt
    54601 atgaacagat gccggaaaac caacgagctg gtctgaagcg cgaagactgg tggtatacgt
    54661 ataaattcaa gcaaaaaggc aattaccagt ttgctttgca ttttgatgat aatgaggaag
    54721 caacgggata tatcgtttat cagcttgcaa cgtcttcctt tattattgcg gagtggggat
    54781 ttttggatca tgatgctttt tgcagccttg ttcgcttcgt aagttctcac aatggtgctt
    54841 ttgaaacatt tgaatattca tgcggctacg gcggtaatga tcaaaattat ctattagaaa
    54901 atccatttgc ttctgtaatg atcacacctt atatgatggc gaggatcgta gatatttctt
    54961 tgtttttgga aaattatcca tttaaaaaga gacaagctgc atttgcccta caaatagaag
    55021 aagatcatta tgcgaaatgg aatgaaggaa tcgtggaagt aacaatcgaa aatggaaaaa
    55081 atcatctctc aaaagtagaa caaacaaaac tcccaatcgt tcgaggaagt atccaacata
    55141 tcacgcagct attattaggg tatcgtaaag tggaagagtt ggtattccaa gaaagaatac
    55201 aggtgacaaa agaatatgaa gaaacgttaa gctcaatctt gccacagcaa cagccaattc
    55261 ttaatgacta tttttgataa aaaacgggtg atgacaatat ttttgtcacc acccttttgt
    55321 ttttcatttg gcatttgaag gaaaatgtta aagtaaatcc tcttttttcg taaatttgaa
    55381 ttttttgttt tcttttgttt tagggttttc ttttttgtgg tcttggattg cttctttcgt
    55441 aatcttttcc cgattttttt tagatgaatc taagcgttct gcgtatactt tttcttttgg
    55501 cttatcattc attggaaagg acctccctac attatttcat tctacctcta gtttatcatc
    55561 tgttaaggac tagtggaaac gttttgccta acttataagc tgattttttt agcagataag
    55621 cttgcaagta aaaaacaaac aagctacaat tgatttattg aaattgttcc tgtagctcag
    55681 taggatagag caatcgcctc ctaagcgata ggtcgctggt tcaattccag tcaggaacgt
    55741 acaaataacc cctgtatcct ttgcggtaca gggggttatt ttgtataagt agacattgta
    55801 gtttctaaac tgctatgtcc taaacgtttc gatacgctaa gtatatttac tccttgataa
    55861 agtaaaatag atgcatgcgt atgtcttagt ccatcaataa acagtcgaag agaaaatagg
    55921 gaaaaattga ttactaaaga aaaacttact aggtcgacgg tgtagttgat tttcatactt
    55981 caatagattc gaattaaacg atttcgattg gcttatacca gtcctatcca acgaaagata
    56041 tatcgttctt aactctagcg gtataacttg aagctttacg taaagccaat gggaatgaac
    56101 tggagatgac tttttattat ttttagtaat agctgatggg ttaatactta caactttcct
    56161 tttcaaagta ccatataaaa aaacggtggg gttgtttagc gaaataaagg atctaagcca
    56221 atagcttaga tcctttattc tatgctttat attatttttt gcatgtgtgc gaaagtatac
    56281 tcaagcccaa tcgccatttc ggaaaatcgg tactgtagag ccatcttcac ggattccgtc
    56341 gatatccatc ttgtcagatc ctaccatgaa atctacgtgg gtttgacttc tattcaatcc
    56401 agcttctgct aattcttcat cggacatttc tgtaccgcct ttcacactga aggcataagc
    56461 agaacctagt gctaagtgat tcgatgcgtt ttcatcaaat aatgtattga aaaagatgat
    56521 gccagattga gaaataggtg aagggtcagg tacaagtgcg acttctccta aacgacgcgc
    56581 accttcatct gtatcaagca gttttgctaa gacatcttct ccttgttcgg cagaaaaatc
    56641 aacaactttt ccatctttaa aagtaaattt catacccgag atgatggtgc cagcataact
    56701 cagtggcttt gtacttgaaa tgtaaccatc tactcgatga ctgtcaggtg ctgtgaacac
    56761 ttcttccgtc ggcatatttg ccatgaattt ttctccacga gcattatagc ttccagcgcc
    56821 ttcccaaaga tggtttttag gcaatccgat aatgatatct gtaccgggag cagtatagtg
    56881 aagggcagaa aattgttctc gattcaattc ttctgctttt tttgctaatt tttcatcatg
    56941 cttcttccat gctaagacag ggtcttcttc atagacacga gtcgttttga aaatctgatc
    57001 ccataatgcc tcaacttgtt tttcttctgg cagttctggg aagacttttg ctgcccattg
    57061 tttaccagcc gcagccacga cagtccaact gactttattt gcttgagttg ccttgcgtaa
    57121 attcattagt gctttgccat tcgctgattg ataagacgct actcgatggc tatctacacc
    57181 agcaaatgca tcgggatcgg cagaaacaac gctgatcctg cttgctcctt tttcaagcca
    57241 ttcatccgct tgatcgattt tagattgtgg tacattctca atacgatccg ttgcagcatg
    57301 taaaagaaat tctctttgga tctggtcgtc agtccattga acgataacct cagcggctcc
    57361 tagtttataa gcttcttgtg tgatcaatcg agcaaggggt gcctgttcca cgctgatctg
    57421 taaaacaact gtgtgtcctt tttctgtggc tacgccagtt tctgcaatta agcgtgcata
    57481 tttttttaga agttcattaa aatcagataa cataataaaa attcctcctc accatttttt
    57541 ctggtaacta ttagcatgat accatttaat tatgattcaa acaatgaggc tttaaataaa
    57601 aaaaggaggt tattttttta aggttaagtt tcatgatata tgttactact tgctatttca
    57661 gcttgtgaag aaaatcaaaa actaattttg catatcaaaa aaacatgtta caatgaaatt
    57721 aatcaatggt ggaagcgtta taaaaaaatt aaggagatta aaaaatatgg caagaaaaaa
    57781 gacgattact agagaacaga ttttggcagc tgcttatgaa gtagctgcca ccgatggatt
    57841 tacacgattt actgcgagaa acatcgcgaa caaaatgaat tgttccacgc aaccgattta
    57901 tcttgagttt aaaaatatgg atgacttgag acaagctctt tttgagcaga tctatgatca
    57961 tttacaatat gaagtgttca ctgttgagca tacagggaat acgattatcg atcttgcttt
    58021 taattatatc cattttgcaa gaagagagaa gaagttgtat cgttcactgt acttagaaga
    58081 atatggtgga ggaaaagaga tgcagaattt ctcttatact tactttgtga atacagtgaa
    58141 acgcgatcca gcttatgtga atctttcaga agagcagatc gaatctttgt ataatgggac
    58201 atggatcata gctactggga tcgcagcttt gatgtcttca agcatcatcc atccaactga
    58261 tgaacagatt gaaaagatga tccaagacag tatagatgct atcttgaaat tcaaagaacc
    58321 aatcgaaatc gattgaatat actcaaaagt tccacaaatc agtaggtaac aagagatgtg
    58381 tcaaaataag gaaaagttcg tttttcttac agcgtatgca tttttaactc ccgaagaact
    58441 cttccatgaa tgcaatgaat aaaagagcca tacagattga ataaatctgg atggctcttt
    58501 tattgcaagt atattaaatt tttgcgacta gttctttagc gaaagcttct aaattttcga
    58561 tatcttcttc ttcagcattc agatccactt taacattttc ggctcctttt gatgcgcctg
    58621 tttttgcaaa agcagcatcg aaatcatcca ccgatttgca gaattcatcg taaaaagtat
    58681 caccagatcc gcagacaccg taaatttttc ccaatagatc caattcttgc aagtcttcat
    58741 aaaagtctac gatctcatca ggcagatcgc catcatcgta tgtataagta gctactacgc
    58801 aaatatctgc ctcctcgaat tcatctgcat ctacttgggt acattcgttg atttcgactt
    58861 cgatatccag attttcaaat gcttctgcaa caatatcggc gatttcttca gtgtttcctg
    58921 tcatgctagc atatacgatt tttgctaaag ccatgatgtg cctcctcgtt tataattatt
    58981 taacaattat gaattatacc aaaacatcat tgaaaagaaa ataaaaccta aagacacctg
    59041 gggaatgtca tttggctaaa tgtatgccgc tatgttaaaa tgagagggat ttgtaaaaag
    59101 gagacgaaaa tatgattact ttaaaatcag cacgtgaaat tgaaatgatg gcagaatctg
    59161 gagctttatt agcagatgta cataaaaatc tgcgtgattt tataaaacca ggaatcacaa
    59221 gttgggatat tgaggtattt gtcagaaact atatcgagag tcatggcgga attgctgcgc
    59281 aaattggctt tgaagggtat gaatatgcga cttgtatcag tataaatgat gaaatttgtc
    59341 atggattccc tcgtaaaaag cctttgaaaa acggtgattt ggtaaaagtc gatatgtgta
    59401 tcgatttgaa aggcggcatt tctgattctt gttgggccta tgtagttgga gaatctacac
    59461 ctgagatcga ccgattgatg gaagtaacga agaaagcttt gtacatcggt attgagcaag
    59521 cacaagtagg gaatcggatt ggtgacatcg gtcatgctat ccaaacttat gtagaaggcg
    59581 aaaatctagc gatcgttcgt gattttattg gtcatggcgt tggtcctacg attcatgaag
    59641 aaccagcagt tcctcattac ggagaagctg gaaaagggtt gcggttgaaa gaaggcatgg
    59701 ttatcacgat cgaacctatg gttaataccg gaacttggaa aatgaaaatg gatcctaacg
    59761 gctggactgc gtatacaaga gacggcggac tttcttgcca atatgaacat acattggcta
    59821 ttactaaaga tggtccaaga attttgacat cacaaggaga agaaggcact tattgatcta
    59881 tccagtcaat aggtacggga gaggtacatg aaagtttgga acaaaataaa acaaaatgaa
    59941 aatctgatgc gctttattga gacgactcaa aagcgcatca ctgattcaga aatgagtacg
    60001 acctctgtcg tcgttgctta ttatttactc ttgtctttat ttccattgat tattgctttt
    60061 ggtaatattt taccgtttct ccacatcgat caagagactg tactgaccta tttacgagaa
    60121 gtgattcctg agaccgttta ccagtttatc ggtcctgcaa tcaaagacct tttgacacaa
    60181 agttcaggag gcttgttatc cgtttctgca ttagcggctt tatggtctgc aagtcaaagt
    60241 atcaatgctt tgcaaattgc gatgaataaa gcgtatggcg tagaaaatag aaaaaatttt
    60301 ttgatcgtcc gtttcttctc actaattgtc attgtcttgt tcatgattgc tattagtggg
    60361 gtaactatcg ttcttgggtt gggacagctg attttggatg ctattcagcc aatcgtacaa
    60421 atacccgtga actttatcga tcagttccaa actttgaagt ggccaatcac attggtagct
    60481 ttatttgtca tcatgttctt gatttattta attgtcccta atgctcagtt gaagttgaag
    60541 acgatcattc cgggcacgat cttcgctact gtcggctgga tgctgttatc acaagtgttt
    60601 agtatctatg ctaggtattt tgcaacgagg atcagcggtt atcagatcat aggaagtttt
    60661 attgtactca tgctttggct taattttgct gctacgatca tcattttggg cggaatcatc
    60721 aatgcagttg ttcaagaata cgtgacgggt catcaaatca aaaaacgtcg agatccaagc
    60781 cgaaaatggt tcaataaatt gaaaactaaa ttttctcata aaaaaaagta gaaacaagag
    60841 ttcttattgt aaatttatat cagtaattta taataattat gactcttaat taaagtaggt
    60901 gcccatatgc ttgtttcttt tagtatggtt ataagatttt ttctgacaat tttattatca
    60961 ctgatattaa cacctgtatt caaaatcatt tcagtacaaa caggtatggt cgacaaacca
    61021 aatgaaagaa gaataaataa agttccaatg ccttcagcgg gcggactgcc tatctttatt
    61081 tcatttgtta tatcgacttt attcttattc cgtaaaatta tccctcaatc gtatattgta
    61141 ccgatcttga ttgcttcttt agtaatcata gtcacaggag tgctcgatga taaatatgag
    61201 ctgacaccta aacaaaaaag tatagggatc ttgattgctt ctcttattat ttattttgta
    61261 gcagatatac gtattgattc cttcacgctt ccttttattg gatatatcca actaggctgg
    61321 cttagttttc ccgtaacgat tttttggatc ttcggtatca cgaatgcggt caatctgatt
    61381 gacggtttgg atgggctggc ggcagggatt tcattgatcg gtttaattac gataggaatc
    61441 attggatatt ttttcctcca tgcttctaca gtgtatattc cagttgttat tttctgtctt
    61501 gtagcaagta ttatcggttt ttttccttac aatttttatc cggcaaagat ttatttaggc
    61561 gatacaggag ctctattcct agggtttatg atggccgtcc tttcactaca agggctgaag
    61621 aacgtgactt ttgtttcatc gatttctttg ctgatcgtca tgggagtgcc ggtaacagac
    61681 acgttttttg ctatcattag gagaaaagca aaccgtgttt ctttttctac agcagataaa
    61741 aaacatcttc atcaccgact aatcagttta ggctttactc ataaaggtgc tgtcctgacg
    61801 atttatgcga tggctttgat gttttctttc acagctatgg tcatgaatta tactggaaga
    61861 atcggaacga tcgtattgat tattgccatg ttgtttgctg caattttatt atttgaattg
    61921 attggtctga tcaatgagaa acatcagttt attttaaaaa cattgcgatt tttagggaat
    61981 aaagagtacc gtacgcaagt aaaacagaaa tatttgaaga aatggaaccg tcatatagat
    62041 gaataacaaa ttaaaccagc cgatccttat tagtattgtg acggcaaaca gtgataaaat
    62101 ttttcggaca ctagataatt ttatttcaac tgtaaaagat gaaacagcag taaaaatccg
    62161 aatttttgac aacaattcac ttcccgagta caaaaaaaga ctaacagagt ataggaaata
    62221 tccctacatc gatatctatt ttcatgatga aaattccgga tttggttatg gacataatca
    62281 caatctgata gctagcgatt ctcgctatgg cattatttgt aacccagata ttttagtgga
    62341 tgaagagaca gttacagcat tggtcttgtt attgaaagaa catccggaat gtgcgatgtt
    62401 agcacctaag attcttaatg aagatggaac aactcagcac ctcatccgtg agcagctggc
    62461 tgtttttgat tacgttttgc gctttgtgcc tttcaaatgg gtcaaaaaaa agtttgacaa
    62521 acgactggct gtgtttgaat gccgaggatt gcctactgat gctaatagtt atgtacggat
    62581 gatctcaggt agttttatgg tagctgatat tgataaattt aaagagataa aggggtttga
    62641 tgagcgtttc ttcatgtact ttgaagacaa cgacttgtgt ttgagttttg aaaaagcagg
    62701 ttacaagtta ttgtacacac ccttttatgc agttatccat ttttacggga agggtgcgca
    62761 tcgaaatgca aaactgtttc gcgtgttttt gacttctatg atgaaatttt tcaataagtg
    62821 ggggtggcgt tttttctgat gaaaccagaa atattagtag tcgttgtgct ttatcagcaa
    62881 ctattcagtc aatctccagc ttatggacca ttaagcaaag cacttagcga aaagcaaatt
    62941 cagcttatta tttacgataa tagtcctcaa aggcagcaaa atgagctgtt tgaaaaagaa
    63001 aatatcgttt atcatcatga tccgcaaaat cctggtttag cggtggctta taattttgcg
    63061 ttgtctcaag cagatgaagg aacacgttgt ctcgtcactc tggatcagga tacgaggcta
    63121 gtcgacgatt attttgaact attgcgtaaa gtaaccttta cagatgaatg tgttgctgca
    63181 gttccgatga ttttttctgg gagccgacaa gtttctcccc tatattcaga tcgttatatc
    63241 aatcgtacag ctcaagcagt agaagttgaa ggcccaacag ctgaacgaat catggcaatc
    63301 aattcaggtg ttgcctggtc tgtcaaattt ctaaaaaaga ttggcgggtt taatcctgct
    63361 ttttctttag attttttgga tcattggctg ttttggaaag tggatcagtt ggaaaaaact
    63421 gtggagatcc ttcctgtacg aatggaacat gatctttcgg ttttagacta tgaaaaagtt
    63481 tcaagtaatc gataccgttc tattttacaa gcagaaaatc gattttatca tgaatatgat
    63541 caaaggcatt tatccagtca ccgacgacaa ttattgttaa gaacagctaa acagttcttg
    63601 accgtcaaaa accggcatat ctggcggatg actttgcgct catatgttaa gaattggaag
    63661 gtgtgaattc gtgcgttcgg tttgtatagc tacctttaat ggaggcagat atttaagaga
    63721 gcagttagat agtattttat ctcagctttc agcagaagat gaagtagtca tttctgatga
    63781 tgggtcagta gatgatacat gggatatatt aactgaatac gcagaaaaag ataagcgaat
    63841 ccagcttttt tcggggcctc gtcaaggatt gatcgctaat tttggttatg cgatccagca
    63901 gacgaaagga gatctaatct ttttggcaga ccaagatgat gtttggttgg agacaaaagt
    63961 gaaaaagatc actgactatt ttgagcagaa tccggatcag ctagtagtag tttctgattt
    64021 aatcattgtg gacagcgagc tgcaagagat ccatgattcg tatttttcgt ttcgaaattc
    64081 caaagaagga tggcttaata atgtgataag aagtcactat attggtgctg gtatggcttt
    64141 ccggtcttct ttaaaacccg atattttgcc gatcccgtca gaagtgccga tgcatgatat
    64201 gtggattggt ttgctgggag gaaagaagac tgggttccta agagaaccgt taactctgta
    64261 ccgaagacat gatttaaacg cgagtgagat tgaaacacat tctaaattac ggcaaaaaat
    64321 ttcttggcgt gttcatctga ttacggcgtt gatcaaacga aaatggttta aacgttgata
    64381 aaagatgttt aaatccttaa tatttccatg acaaatataa caattgatga gataataaca
    64441 aagtgaaatt tgaaatcaaa aaggagagca gatatccatg aagggaatca ttcttgctgg
    64501 gggcagcgga acacgtctgt accctttaac aaaggcaaca tcgaaacaat tgatgccgat
    64561 ttatgacaaa ccaatgattt attatccaat gtctactctg atgttggcgg gaatcaaaga
    64621 aattctgatt atttcaacgc cacaagatac accacgtttc aaggaattat ttggaaacgg
    64681 aaatgattta ggactgcaga tcgaatatgc agtacaagaa agtccagatg gattggcaca
    64741 agcgtttatc attggagaag aatttattgg agatgatagt gtctgcttgg tattagggga
    64801 caatatttac tatggtggcg gtctgtctaa aatgttgcaa cgagcagctt caaaagatac
    64861 aggcgcgaca gtatttggct atcatgtcaa tgatccagaa cgctttggtg tagtagaatt
    64921 tgatgagaat atgcaagctt tgtcgattga agaaaagcct gctcagccaa aatcaaatta
    64981 cgctgtaact ggcctttatt tttatgacaa tgaggtaatt tctatcgcta aaggtatcaa
    65041 accttcagaa cgtggagaac tagagattac ggatgtcaat aaagcatatt tggataaagg
    65101 aaaactatcc gtagaactta tgggacgcgg atttgcttgg cttgatacag gaacacatga
    65161 atcattgctt gaagcatcta cctttatcga aacaattgaa aaacgtcaga acttaaaagt
    65221 ggcttgtttg gaagaaattg cttaccgtat gggctacatc gataaggaac aattgctggc
    65281 ccttgcacag ccattgaaaa aaaatcaata cggtcaatat ttattgaatt tggccgctga
    65341 ataaggagtc cttacagatg aaagtaacag atacaaaatt aacagatgtc aaaatcgtag
    65401 aaatggacgt ctttggcgat catcgcggtt tcttcacaga aagttattca aaagcaaaat
    65461 ttgcagaaca tggattgaaa tatgactttg ttcaagacaa ccattcacta tcagctgaag
    65521 ctggtgtcct tcgggggctt catttccaaa aaggagaagc tgcacagaca aaattgattc
    65581 gggtagtcac tggagcagtt ttagatgtag tagtggatat ccgcaaaggt agcccgactt
    65641 atggtcaatg ggacggattt atcttatctg aacataatca tcgacagttg cttgtaccta
    65701 aaggatatgc ccatggattc gttactttga caccaaatgt gaacttcctt tataagtgtg
    65761 ataattacta taatgcagaa gcagatgggg gcatcgcatt tgatgatcca gatttagcga
    65821 ttgattggcc aattgatcca tcaagagcga ttatgtcgga aaaagatcac cgtcatccga
    65881 cattaaaaga atttgaagca gaaaatccat ttgtttacgg agaaatctaa gaattaagga
    65941 gaaattttct tatgaaaaac atcattgtta ccggaggtgc cggattcatc ggctctaatt
    66001 tcgttcatta cgtggtaaac aatcatccag aagtgcatgt cactgttttg gataaactga
    66061 cttatgcagg aaacaaagag aacttggccg gacttcctag cgatcgcgta gaattggtcg
    66121 taggagatgt tgcagatgca gaattagtgg accgtctagt aaaagaagcg gacgcagttg
    66181 ttcattacgc ggcagaatcc cataacgata attcattaaa agatcctttc ccatttgtac
    66241 aaacgaatat cattggtacg tatacacttt tagaagcttg ccgcaagtat gatgtacgct
    66301 atcatcacgt atctactgac gaggtttacg gcgatcttcc attacgtgaa gacctgccag
    66361 gacatgggga aggcgaagga gaaaaattca ctgcggaaac accatataat ccttcaagtc
    66421 catattcttc tacaaaagct ggttcagatc tgttagtaaa agcatgggtg cgttcttttg
    66481 gcttacgtgc aaccatttca aactgttcaa acaactatgg tccttatcag catattgaaa
    66541 aatttattcc tcgccaagta acgagtatct taagcggtat tcgtccaaaa ttatatggcg
    66601 aaggaaagaa tgttcgtgac tggatccata cgaatgatca ttcttcagca gtgtggctga
    66661 ttttgacgaa aggcagaatt ggcgaaacct atctgatcgg tgcagacggt gaagaagata
    66721 ataagaccgt gatggaattg atcttagaaa tgatgggcca accaaaagac gcatacgacc
    66781 atgtaaacga ccgcgcaggg cacgatttgc gttatgcaat cgattcgaca aaattgcgag
    66841 aagagctagg ttggcagcct gagtttacga acttccgtga tgggttggct gacacgatca
    66901 aatggtacga agaacatgaa gactggtgga aaaaagaaaa agaggccgtt gaagcagcat
    66961 atgccaaaaa tggacaataa tacagaggaa atttcactgt attaatggaa aaacacatgt
    67021 gaggcaggcg ctcgcctgtc tcttttttaa aataaaaatc aaggagcgga taaccatatg
    67081 tttttattaa ctggaggaaa tggacaatta ggtacagaat tgcgccattt attagatgaa
    67141 aaaaatctgc catatgtttc aacagatgca aaagacttgg atatcacaga tgctgagaaa
    67201 acaatggctt atattacaga attaaaaccc gatgttattt tccattgcgc agcttacaca
    67261 gcagtagata aagcagaaga agaagcaaaa gaattagatg agaaaatcaa tgtcgatggc
    67321 acaagaaatg tagcacttgc agcaaaagct gtgaatgcac aattcgtcta tatcagtaca
    67381 gactatgttt tcgatggaaa aaacaaaaat gtagaatacg aagtagacga ccaaacgaat
    67441 ccactaaatg aatatggtcg tacgaaatta ttaggcgaaa aagcagtaga agagatttta
    67501 gaagactatt atatcatacg aacttcttgg gttttcggga tctatggaca caatttcgtc
    67561 ttcacgatgc aacgcctagc ggaaacccgc gacaaactga ctgtagtaaa tgaccagttt
    67621 gggcgaccaa cttggacacg tactttggca gaatttatgg tatatgtcat cgaagaaaaa
    67681 gcaccatttg gtatttatca tttgtcgaac gaaaatagct gttcttggta tgagttcgca
    67741 aaagaaatat tgaaagatac agatgtagaa gttgcacctg ttacgtcaga agaatttccg
    67801 caaaaagcaa cacgtccgca gtattcagtc atgagcctga aaaagacaga agcgttaggt
    67861 tttgtcatac caacttggca agaagcactt gcgcaaatgc tggaagctgc caaaaaaatg
    67921 aataagtaat ttccttttta tcaaatagaa tttggagcat gatatggaaa aacaaacaag
    67981 aagtgcacga cgaaaagaaa agccaaaagc gaatcaggca gattcgaatt tggtgcttga
    68041 cctgtttcta aaactccgct atcttattgg cgttttagtc atcgtggtca tcgttacttt
    68101 taatttaaac ggaagttcac ttggtgtttg ggataaatat gtgtcgcaaa gagacgatgg
    68161 gaaaaaaact gatgtgattt ttggacagaa tcgggaagtg cggtcagacg aatggctggt
    68221 gcagacacct ttttacctct cacaagctga agaggacttt tcgttggtga acgaggatta
    68281 ttcattaaac ggacaaaaca tgatcattgc gtataattct cctgtaaagg atattacggt
    68341 tatcggaaaa ccattcaact ggggattctt tttcctcgga agagatcgag gactttcctt
    68401 ttattgggcg atgaaattag tcgtcatggt cttgcttgga ttcgaagtac tgatgatatt
    68461 aacgaagagg aaaaaagctc tatcgctaat cggtgcattt gcgctgacat tttctccagc
    68521 agtccagtgg tggttcatgc aacatgtggg cgatttgatt ttctttactt tgggactgat
    68581 gattgctttt tatcattatt tctacaatca tgataaaaaa tggctacgaa cgttgatgat
    68641 gcttttggtg gttattttcg gtattgggtt tattttagtg atttacccag cccatcaagt
    68701 gatgctggcg tacttgctga ttttttattt tgttggcctg cttatttact atagcaagaa
    68761 agtttcatgg gatctgttgg atgtagtgtt gattggaggg gcagttttat ttattggcgg
    68821 agtcatggtt catttctggc taacgtcaaa agacgcattg ttagcttctt tgaatacctt
    68881 atatccagga aaacgggtaa gtactggagg aaagtggacg attggcgaat ttttttcttt
    68941 ccttacaaat tggaaaattc cttttgaaga tatcaactat tcgaataact ctgaagtggc
    69001 cctgttctat cacttttttc caactgtatt cctagcaagt ccatttgtgt tatttggcaa
    69061 aaaagatagc gaacaaaagt tactgggacg tattttgatg attttttgtc tgttttctat
    69121 cttttggatt accgttgggt tgccaaaatg gctagcagaa atcactctgc tgtcttatgt
    69181 tcctccatta agagccatcc ttacttttag ttttgcggct tgtttattat cgatctggtt
    69241 tatcgcttat atttggagag aagatgttat tcctatttgg tataaattca ttttgttggc
    69301 agtaaatgca tggtcttatt tctatgcgat tacgcattcg aaaatggata attatttttc
    69361 ggattttgaa accatcgcag ttgctgtgtt gggaacagtg attttagctt ttgcattatt
    69421 tggctggaga cgcttgtttt acctcgcttt tgtcgcctta gttatcgtat ctggcttttt
    69481 cgtcaatcca gtcgtagaag gtacaggagc gatttttgaa aaaacactgg caaaagaaat
    69541 ccaaaaaatc gatcaacaag atcctggtaa agtttggttg tctgaagatg aattatataa
    69601 cttcacccca acattgggag tgaaatcctt caatacagta cgcttttatc cagatatagc
    69661 tgcatggaag aaaatcgatc cgaaagaaga atacgagaag tattataatc ggtatgctca
    69721 tacaagagct tttatcgcta aggaagatac aaagttcatt ttagatcgtc cagatatgtt
    69781 ttcggtcacg ttgaattttg aagacgcaga aaaattaggg atcgactatg tgatcagtaa
    69841 acgaccatta actgattaca accaattata tgaagcgcag ttcacgcagc tatatggacc
    69901 ggataaagac ggttatatga tttttaaagt agaatatccg gattatccag atgtaccaca
    69961 aacacaaact caggaaaatc agctagtcgt acagtaatag gctagctgag aataatcctg
    70021 aaatattagt agaaaggaga gtgaagataa ttcaagcaat cgtacaaaaa tttgtcaaca
    70081 aaatgagtgt cggtttcttg cttttgttgc tgacgctagt cttttttgtt tggacagcag
    70141 gggtaggttt tccagcaact ttgtttatat ataacaatag ttcattactt atcgctttta
    70201 gtgtggctat tttgctcatt ttaaatgttt ataaagcaaa aggcgtcgat tttctttatc
    70261 taggtgttgc gcttgccctt ttcattttct tttcttactt tcgggaatta agaaatgagt
    70321 ctttatttgg agacttactg tttcctttgg ttattttatt ttgtttagta attaaatgga
    70381 tcgagtggac aaaatgggat cgcggtattt atctgattgt ctttacggtc ttcctcggaa
    70441 tcacgttata tcgtgtattt acagagatcc aagtaccaga aggacaaagc atctgggctc
    70501 caggcaattc gctttctgat atttggatca atacgaatac aatcggaagt tctttgatga
    70561 ctcttgcttt attgatcagt ggatttgcca gttcttttga acgatggtat gttcgaatca
    70621 tcggtgggat agcagtcgtt gcagcctttt taggtatttg ggtctgccaa tctagaggtg
    70681 cattagtcgc gttgattgtt tttgcgatat tggatctttt acctaaatcg ttgatgcgtg
    70741 tcattcgtgc gccatttatt gcctacacgg ttacgattct tttagctttg ccgatttctt
    70801 atctggcggc tgtctctgaa aaagtcaatt tgtttaccga tcgagaagat atttggcata
    70861 aattctatca gacgctagga gaaaaatctg aacaagtttt actaggaatg aaaacattta
    70921 tcttccagcg aggaaaccaa tttttaggaa accacaatag ttataattct attttgaata
    70981 tctatggatt gataggattt gggattgctg ctttgctgct tatccttttt attggtcgct
    71041 taacgttgaa agcagattta tccaacgggc agatgacttt tatctgggcc ttttttgcgg
    71101 tgatgatgca gtcatttatg gaggatacat tgacttcata cccatgggtt ccgattattt
    71161 atatcctgtt ggctatggcc gttcatcgat atgatgaacc agaagttgcg gaggaattct
    71221 atgttatgga agaggcatat gacgatatga cttatgatga tacatcttat gaggaacaac
    71281 cagatagcgc tcattcttcc aggatccaac gctatcattg ataggcacaa aatacgagag
    71341 aggctgtgac aaccgtcgca gccttttttg gtttattgca attgtctagc atcttctgta
    71401 aaatagaaac gttagagaaa atcttgtatc atagaaaatt aagaaagaaa taagaacgca
    71461 gctcggtacc aaaaatatgg taaaatggca cttgactgta attcactgaa aatcgcttgg
    71521 aggaacacaa atgtttaaag atttgtattt atttatgaag gacatttatc agaacagaaa
    71581 attattgcta cagttttcga tcaatgactt caaatcaaga tatgcaggat cgtttttagg
    71641 aatcttatgg gcgtttatca atccgctatt tactgtattg atttattggc tggtatttgg
    71701 tgttggactg aaagcacaaa tgatcgatgc aaaataccca tttatcgtat atttagttac
    71761 cgggatgatt ccctggttct tcttctcaga tgcatttgca tcaactacgg ttgtttttcg
    71821 ggaatatact tatttagtaa agaaagtcgt attcaatatt cgtatcttgc ccacgacgaa
    71881 aatcctatcc aatctgtaca cccatttatt cttcattctg attggcatag ctgtcgcgct
    71941 tataaatggt atatatcctt cagcaatgtc tttacagctg atttattatt tattttgcat
    72001 gtgtgctttc ttgacaggat tgacttggct gacggcatcg atccaaccat ttttacctga
    72061 tatcatgcaa ttaattacga tcgtgctgca gttgatcatg tggacattac cgatattatg
    72121 gagccctaat cagtttgatt caggtcttgt aagcatattg aaattaaatc cgctgtacta
    72181 tgttgttcaa ggttatcgtg agtcattttt aagtgaagct tggttctggg agcatggaac
    72241 atatactttg tatttctggg ttttcacgtt agtaatgttc gtaatcggct ctgcagtgtt
    72301 ccgtagattg aaaccacatt tttcagatgt gctgtaagga gaatagctat gacagaatat
    72361 gcaattagac taaaagatat cacaaaatct tataatatgt acgcaaaacc ttcggatcga
    72421 tttcgtgaag ccctcaatcc ttttaagaaa tcctatcatg atgtatttta tgcgttgaaa
    72481 aacgtgaatg tagaaataaa aaaaggtgag atgatcggtt tcgtgggaga aaacggatcg
    72541 ggaaaatcta caatgttgaa aatcattacg ggagtactga caccaacatc cggcacgatg
    72601 gaaatcgaag gaaaaatctc agctttgcta gaactaggat caggattcaa tcctgagtat
    72661 tctggctatg aaaatatcta tttaaatgga atggttctag gattttcaag agaagaagtc
    72721 gatgaaatgg tcgatgatat cattgaattt gctgacatcg gagaccatat ttatcaacct
    72781 gtaaaaactt attcaagcgg gatgttcgtt cgtctagctt ttgctgtagc aatcaatgtg
    72841 aatcccgata ttttgattgt ggatgaagca ttggcagtag gcgatcttga atttcaacta
    72901 aaatgtatgg aaaaatttac agaaatcaag aactcgggaa agacgatttt attcgtttct
    72961 catgatatca gttcgattcg tcgtttttgt gatcgttcct attggttgaa aaacggagaa
    73021 gtcgtcgaat atggcgatac gatggaagta acgacgaatt atgaaaactt tttaaagaaa
    73081 aaatcagtta aaacagtgga tcggaataaa aaccaagtag aacggttcgc cgctccagat
    73141 atcgtcgacg tgacgaaagc ggaattgttg gataaagaca aaaatccggt ggatatgctg
    73201 gaacaaggag aaaaactata cgttaaagta acttatgaag taaaagatga tacaatcaaa
    73261 aatccggttt taggaattgc gctacgaaca gtcgacaatt tttatgtatg cggacttaat
    73321 actctactag atggtatcaa gattccttgg aaaaaaggag agaatatctt ttatctggag
    73381 tatgaaaagt taggtttgtt agctggtgaa tattattttg acgttgcggt ctttgaagaa
    73441 aatgcaacgg ttcctttggt atataagaca aagtacatga acttgtttgt cagcggttct
    73501 tatatcggag aaggcatcgt ggtcttagac cataaatggg aagaagggac tcatagcaat
    73561 gaaatatgat tttgaaatgg acctagatga acaaagcagt gtgggaaaaa ttgccgcaca
    73621 gatcaaacca ggaagtaaag ttttagagtt tggtcccgga aacggccgat taacgaaaca
    73681 tttaatcgga gcaaaacaat gtgaagtgag cattgttgag ttagataagg aattgttcga
    73741 ttttgtcagc gaatttgctc aagatggatt ttatggtgat atcgaaagtt ttgagtgggc
    73801 aaactattat gctggacaga catttgatta cgtcttgttt gcggatgttt tagaacattt
    73861 ggtagatcct ggaaaaacat tgaagaaagt cagagaattt ttgaatgaag aaggagagat
    73921 cctgattaca ttccctaact tggcgcataa ttctgtcatg atcgatttgt tcaataacca
    73981 attgccgtgg gcttcttatg gtctcctaga tgaaacccat aattcttttt atactcatga
    74041 tggcttccaa aaagtttttg aaaaagcggg attattcatt aatattgaag attatttgta
    74101 cttagctgta ggtgatacag aattaaaatc aacttatgaa gaattgccgg aagctgtacg
    74161 ctatgatttc aaaatgcgac catttggtga agtctaccaa tactttttct cattgatgaa
    74221 acatccagta gcacaaagtt ccattgcaga gccacaaaat tctaattatg taaaagtgtt
    74281 agaagtaacc cagcaaacga aacaagatga aacaatccaa caaataccat tcaataattt
    74341 cacaggggaa aatgagacac taagtttccc cgtatcagaa acaacagaaa gaatggtgtt
    74401 tcgcttcgcg caacaaccta gctttatcga attcagcgca gaagctgccg gagaaaaact
    74461 aacatttatc gattcgaatg ctgtagtcaa aacagtaaat gattgttatt tgtttgatgg
    74521 gaaagaatta ccagagttcg ttctaacgga tatttctggg aaagaagtga ctatccattg
    74581 tcactatcgc tttatcgggg aattgacacc aacaatgaaa gaattgttgg aaacgattcg
    74641 tcccatggct gaagtcacaa agcagctttc tgagaaaaat gacgaattag aaagagaaaa
    74701 tcatcatctt cttgaggaaa atacaagact agaccatacg ttaaaaacaa ccacaaatcg
    74761 ctattgcaca ttgttggaaa gtgatgaatg gacaataaaa catagactag gacgacgtaa
    74821 gaaagaaact tcgaaaaaaa tccaagaaaa agaactttct atatgtattg acgagaaaat
    74881 ctgggatgca gaaacaaaaa tattaaaaat catcggatgg ggaatcgcta attcggatcg
    74941 ccagcccttg tcctataagt tatcagctga ccaatcgcca tttttcgaag caattcctgt
    75001 ttcacgagaa gaagtcaatc aagcagaaca gctcgcaaaa ggaacagaag cgggatttga
    75061 gttacgaatc ctttgcgagc aagaacgttc attcttagtg gaagcagtag ctcaaaatgg
    75121 ccaatcatgg atcattgaaa tgtaaataat gattaactta taaagataag gagtaaacga
    75181 atcgtgcaaa aaaaagaaat tgccgtattt atcgatcaaa tcactcgtga acgggccact
    75241 ggcgatttgc ttattgtggg ctgggcaatt gatgaagtga ccaaagaaat ccctacgata
    75301 aaggtagaaa aagaaaatgt gattgctgaa gcaacacatg tcgtaagatt ggatatcaat
    75361 catttatata atcttgatgt taagacccaa agcggtttca aggtacgttt atcagggaaa
    75421 atgagaggaa aagccatctt agacttccaa acagcgagac atcaaaatgg catagctgtg
    75481 aaattaaacg gtaaataccc ttatgatgac ggcattgaat cttcatggga aagaaaaaaa
    75541 agattactga aaaaagggat aaattatgca aggactcatg gcgtaaaaaa agcgatccgt
    75601 cgggtcaaat tggaattgaa gccaggatcg atcgactatg cagagtggat cagtcgtcat
    75661 gagaaaccag atttgaaaga gcaaaggaaa gaagcacaag gatttgcgta ccgtccattg
    75721 atttccatac ttatgcctgt ctataacgtt gaaatcaaat ggctcgaaaa atgcattgat
    75781 tcggtactgg atcaaacgta tgatcattgg gaactatgta tcagtgatga tgcttctact
    75841 gatccagcta tccgaaagtg tctggaatcc tatcaagcaa aagatgatag aatcaaagtc
    75901 gttttccgaa aagaaaatgg acacatcagt ctggcaacta attctgcatt ggaaatagca
    75961 gaaggagaat ttatagcact cttagataat gatgatgaat taccgccatt tgcattatat
    76021 gaagtggcaa aagtgctaaa cgtacatcca gaactggatg tgatctacag tgacgaagat
    76081 aaaatcgatg cagatggcaa tcgctttgat ccgcatttca aagcagactg gtcgcctgat
    76141 acattgatgg gaaataatta tatctctcac ttaggcgtat atcgaacaag cattgttaaa
    76201 gaacttggcg gtttccgtaa aggctatgaa ggttctcaag attatgattt ggtccttcgt
    76261 gtgacagaac aaatccctgc agatcatatt taccacattg accgagtctt gtatcattgg
    76321 cggacgatcc caggatcaac tgcaagtaat ggcgaagcca aaagttatat ctatgattcc
    76381 ggagtgaaag cattgacaga tgccctgtct agaagaaaca tcaaaggaag cgtacgtcct
    76441 ggcagaattt ctggatttta tgagattgct tatgacgtct tgcaagagga tttggtcagt
    76501 gtgatcattc caacgaagaa tggctatgaa gatctaaaaa cttgtgtgga ttcgatcatc
    76561 gaaaaaacgt cttattcaaa ttatgaaatc attattgcgg ataacggaag tacagatcca
    76621 aaaatgcaag aattatttgc agaatacaag catcagctga aagaccgctt tatcgtagaa
    76681 ttgattgaca ttccattcaa ttattctcga attaacaatt tagcagcaga aaaagcaaac
    76741 ggcaaatact ttttattctt gaataacgat acagaagtga ttgagccaga ttggatgacg
    76801 gtgatggttt cttatgcaca gtttgatcga atcggctgtg taggagcaaa actgttttat
    76861 ccagatgata caacgcagca tgcaggagta ttattaggaa tcggtggtgt tgcagggcat
    76921 gcattgaata actatgatcg aacacattgt ggttatttcg gccgattggt cattgacgtc
    76981 aattacttag cagtcactgc agcttgtatg atggtcaagg cagctgactt caatgctgtt
    77041 aacgggtttg atgaaacatt agaagtagca ttcaacgatg ttgacttgtg cttgaaagtt
    77101 tatgaactgg gtcgttacaa tgtctatgca catcaagcag aattgtacca tttcgaatct
    77161 aaatcaagag gttatgaaga tactccagaa aaacaaagac gttttgctgg tgaaatcaaa
    77221 aaaatgcaag ataaatggcc agcatatatt gcgcatgatc cattttacaa tgataattta
    77281 acaaaagacg gaatcggcga cttttcgttg agaccagatt aaactataat aaaaagctgt
    77341 tatgttgtga ttcgctcgca cataacggct ttttatagtt ttttgtcaaa aaaaggcaga
    77401 aggtgccgac tcaaaaaaat aagtcgaggt tgggacagaa gtgtttaact ccgagaaata
    77461 agaagaaatt cacgaaaatt gcttttcaaa tttttgtgaa tttcggctta tttaagaaga
    77521 agttgcttct gctcccaccg tttattcgtt tttaaagaat gagacaaaag tgtttcttac
    77581 ttttgtccca cgctcttatt catgctcaga gcgccaatag atacaaaatc atagccaaag
    77641 tttcacgaac cctagctgtc aaagaatcga tattttattt taatataggc tattttaaaa
    77701 taaatggttt aaggtattcc tctacaaaag ccactctatc aattatcatg ggtttaaaat
    77761 aagaagtgat ggagataacc agattgttct tagcgtcaat ataaagaaca tttccactat
    77821 taccaattgc tgaatatatt tcgttctcag aatctaatac ccaccataaa tagccatatc
    77881 tcatattttg taagtcttct aaagcactgg ttctaggttt gaacatctcg tttatccaca
    77941 tgggtggtac gatttgtttt cctttataca tccctttatt taggcataat acaccaattt
    78001 ttgccagatc acttgccgat aaacacaaac catatcctgc cgtcccgata tttagtggat
    78061 cacaaaacca gacattggtt ttaggagact tatccattgt aaactgctta tgttcttcag
    78121 ctgtttcagc ataataattg acatggtttg gtatccctat aggatcaaat agatacttat
    78181 tggcaaaatc aacagttgtt agcttacttg tctgatacaa caagccagtt aatatgtgta
    78241 aacagacagt ttgatagttg aattcgccgg ttataccttt tcttcctccc aataaatcta
    78301 gtgatgtttt tgtccaatct tcacttgaac agacttttgt ccatggatcg cctttacatt
    78361 tgtagggtgc tttcattgtt agcaagtgct tgattgtaat agcgtaaata gttttctccc
    78421 cgcgtttcac tttatactct ggaaaataat ctaatacttt atcatcgata cttttgatta
    78481 atccttgctc taaagcaatg ccaatcagca aagatacaat acttttcgtt gctgacatga
    78541 tatgacaagt atcgtctttt ttataaccat tccattcatc tgtataaact tctaccccat
    78601 ccttatatgc gacaatttga catatatttg gttgtcgaaa accaataaaa tcatgcaact
    78661 cttgtttatt cataagatat tacctcactt tcttctataa gcataatgtc attaaaaatc
    78721 gatttcaatt ttttcgattc ttcacgtaat acaggtattc ttatggacta gacagcgctt
    78781 tgggaagatg gattctctga aaaatataaa ggttcacttt atgagtggat aaaaaaagca
    78841 gcaaatactt cgacttcttt aatttaccaa cattagtgaa aatcgattca tctggagatt
    78901 tgtaatttag agtccttgtc ttgattagag gtgtataaag ctttttatta gacattttta
    78961 aattccatta caatttgctt ataaactagg tttttaaagg tgactatggt aagataatag
    79021 agatattatt ttgtgattag gagtgtatat atgaataaaa atggagaatg gagtgctgca
    79081 cgacggatat tcattatatt aatggatgtt attgtataca atgcagcaat atatagtagt
    79141 ttcttactga agtttcgtgg agagatccca ttacgaaatt tagaaacttt ccaacactca
    79201 gcaattatta tttcagtgat ttttattgcg ttgaacattt tgttgggtgc atatgttttt
    79261 tataatcgaa tgatcagtga catcgtgttt gtcacagtca tcggtcaggt attgatgaca
    79321 ctagggatca tggtagtgac ttttgcgggc cgctggtttg cttttccacg tcttgttatc
    79381 ttactttcat ttgttctcgg tacgcttttc ttaagtatct atcgtattct cgtatataaa
    79441 ctttatttac gcgtaagtcg agataaaaag gtcgtcatta ttgggttaga aaaggatgtc
    79501 gcaccagcaa tccaaaattt tggttcaaag aaaaacaaca aacataaagt cgaagcagtc
    79561 attcttgata actattatgc aaacatcaaa aatatgatta aagacatcga tattgtctat
    79621 cttgcttctc atatcgaaga aactgaaaaa atcaaaatct atcaattagt tactaaaaat
    79681 gaaaaaaaat tgtttttgaa tacaacattt gaaaacttga cattgatcaa tccaaatatt
    79741 atgaatttcg aagatgaaag tattattgaa gcatctggat ttagaatcca acctgaatat
    79801 gaactattca aacgtttgtt tgatatcatc gtttcacttg ttttattagt tattgcttca
    79861 ccatttatgt tattcacagc aattttagtc aaagtgacat caccgggacc aatcatctat
    79921 aaacaagtgc ggatcacaaa aaatcaaaag gaattttcaa tctataaatt ccgctcgatg
    79981 accgccacag ccgaagcaaa atctggacca gttttagcta agagcaatga tgcacgagta
    80041 acgcctgtag gtaaatttat tcgagctgtc cgctttgatg aattaccaca aatcttcaat
    80101 gtgctgaaag gcgatatgtc gatcgtagga ccacgtcctg aacgttcttt cttcgtcgat
    80161 cagttcaatg cagaaaaccc ttattactac ttacggcata atgttcgtgc tggtatcact
    80221 gggtatgcac aagtctatgg aaaatatgtc tctgattaca acagtaaatt acgttttgat
    80281 ttactttata tcaaaaaata ttcattgatg cttgatttaa agatcttgct gcaaaccatc
    80341 aagatcctat ttgataaaat gtcctctcaa ggacttgaag aagacgaaaa tgtgatctca
    80401 ttggcagaaa ttgcttttcc gaatgaaaaa atctttcgct agttgggagg cgtaaaaatg
    80461 aacaatccga aattcagcat aatcgtacca gtatacaatg cagaagcaac tattggtcga
    80521 acgattgata ttctaaaaga attggattat gaagagtacg aagtcgtttt agtaaatgat
    80581 ggatcaacag atcaaacaca ggaaatcatt caagaactga ttgaaaatga tgaacgtttt
    80641 gaattgatta ctacgaccaa tcaagggccc ggattagcaa gaaacgaagg aataagacag
    80701 tcaaaaggag aatatctctt attcttcgat gcagatgaca cgccagtaaa aacgatcttg
    80761 tcagattatg atcatttgct agaaaagagt ccaaaagcgg atcttgttgt tagttcgttt
    80821 gtttttcgta caatggacga gggaagaatc gtttccgaaa aagaaaactt agttacagaa
    80881 tgtcactatc taactcacga agagtttata aaagacatgt atcagttaat gaatgaacag
    80941 ttgatgtatg ttgtatggaa caaatgctat cgtagcgata ttctgaaaaa taaccaaatt
    81001 ctatttaaaa cttataatag ttgtgaggat cgaatattta acttacatta ttatgaatac
    81061 tgtcatcaag tgatcatgaa cccaaaagta gaatatattt atgaatttga aggtgggaag
    81121 gggatcacta atcaataccg acctaacaag ttcgaaacat ttaaagaatt ctattcatta
    81181 gcaaatactg taacagacga tattaataaa gacggtatgg cggcgcttct tttaaaaggg
    81241 acaacttcag ttattttttc tatttatagt acctctgcaa gaacttcaaa agagaagaaa
    81301 gacgaagcaa agcagatatt gagtgatcca gtgatccgag aagccaagca aattgctaag
    81361 acggattctt cagcaaaaaa aataacaaag ttgctttaca atatgccagc tccactattt
    81421 ttatctgctg taaaagttgg aagcttcgtt gaagtgaaaa tgccagggtt aatggctgct
    81481 ttgaagcgag tatattagtt aatggaggaa aatataatga aaagaatttt gcattttcag
    81541 ggacgaatgg gtttaggtgg agcagagtct ttcatgatga atttatatcg taaaattgat
    81601 cgtacaaact atcaatttga ttttttgatt tatgaagatt atgcagatgt tcaagactat
    81661 cattcagaga ttgaaagatt aggaggccgt atttttgttg tacctaatcc taagaaaaat
    81721 atttttaaat atttaataga agtgaataag ttactaaaaa aagaatcttt ttctatagtt
    81781 cacaatcagg tttattttgg cgggggaatt aatttatggt tagccaaaaa gaatggaatc
    81841 agacagagaa ttgctcatag ccatgcaact gaagatggaa aatctcaaaa tattgtaatg
    81901 aatgtattgc gtaaattttt aaccaaattg ttgttacaaa atgcaacaga ctaccttgcc
    81961 gttagtcaag aagctggtga aagtctgttt caaaaccatc cctttgagat agttcataat
    82021 ggtattgatt tagagcttta tagtaaaaat tcagaagcta aggttaataa gagaaaagaa
    82081 ttggatatct caatgagtac ttttgttgtg ggaaacattg gacgattaga gaaacaaaaa
    82141 aatcaatctt atttgttaga gattttttta gaaatactaa aaatgaattc tgattcttac
    82201 ttgattttag taggtgaagg gtcacttaga aataaactga aagccaaagc agtagaattg
    82261 gatatagaga aaagagtaaa atttttagga gaaagaaacg atattcccga gttattatct
    82321 gtgatggatg tttttgtaat gccttctctt tatgaaggtc tgccaatggt tggattagaa
    82381 gcacaagcag caaaattaaa attagtctta gcagatacta tttccaaaga tacaaagtta
    82441 acaaataatg tggattttgt atcattagat gaaacaccac aagaatgggc aaaaaaagta
    82501 ataaaagagc cgtatgctaa taaaataaca caagaactaa agagttatga tgtttcgt
//
LOCUS       DACDKM010000003        71070 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20834_2016
            SAMN12640370-rid9891663.denovo.003, whole genome shotgun sequence.
ACCESSION   DACDKM010000003 DACDKM010000000
VERSION     DACDKM010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640370
            Sequence Read Archive: SRR10040891
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 71070)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 71070)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742835.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742835.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:04:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,012
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 29 of 75
            Pseudo Genes (incomplete)         :: 33 of 75
            Pseudo Genes (internal stop)      :: 19 of 75
            Pseudo Genes (multiple problems)  :: 5 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..71070
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640370-rid9891663.denovo.003"
                     /isolate="E20834_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            113..856
                     /locus_tag="IV334_000171"
     CDS             113..856
                     /locus_tag="IV334_000171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP8942275.1"
                     /translation="MIEFINVSKSYGKKQALKDLSLSIRQGEIFGFLGHNGAGKSTTI
                     KSLVSIIEPSSGTILVDGMKLTENRLSIKQKIGYVPDSPDIFLQLTAGEYWDLISAAY
                     ELDTQKKEKRLAELTALFDMYSHQNETIASFSHGMRQKTILIGTLLPDPDIWVLDEPL
                     QGLDPQAAFDLKEMMKAHAAKGKTVIFSTHVLDTAQQLCDKLAILKKGELIYQGAVSE
                     LLNGSPDETLEKIYLKMAGRQATGDGPYV"
     gene            849..2429
                     /locus_tag="IV334_000172"
     CDS             849..2429
                     /locus_tag="IV334_000172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter"
                     /protein_id="HAP8942276.1"
                     /translation="MYKRQLLELIRVNLLYVNPQLTNKAREKGKKGKRLTIYLLSQNL
                     FSGLIFLLIYGFAMFAMNFVKLPGFFTYYVALFGMLGLTQSISVIYNIFFEGNDLQVF
                     LPLPFGQEQVFSAKILVVALTIIPFVFPLLVMFILTGWRADLGILLTIIISILLFSLF
                     LIILFCLASLIVFGLARTAVFKKHRKTVSSILLGSSMLIAFAGIMWMNHQTGTIENEL
                     TDRHPISILLPFYDAASQPLSQQGLLGWGILAITALGFFLAVKQLIVPKLTEQLTDSN
                     SEFKIKRTHKKNQNVHQLLFSYNAQLVKEPNLIMQVLSSSLLTPIIFILAFALGGEIK
                     LTDLDNKLIGVVFLVGIALAFLTVNQTSFISNLISLDQENFLFIRSLPISMNQYLKEK
                     FRFGWILQSFLTGGIALLSGLSFQLPLFFIPSLLFGSLFGCYLLSLRYFARDYRLLLL
                     NWTNINQLFNRGSGSLGLVATMMGSLIISIILIVLYGMLALFYPFWLVNIPVIGLVLI
                     LSALWIVYYQRTFWKKFE"
     gene            complement(2680..3159)
                     /locus_tag="IV334_000173"
     CDS             complement(2680..3159)
                     /locus_tag="IV334_000173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter"
                     /protein_id="HAP8942277.1"
                     /translation="MGTIFIQFSFSFLATAAFAVITNVPRRSLICCGLSGAFGWMIYW
                     ICVQIGGSAAFGSLLGSLGVAFISDLFSKRLKMPVTIFNIPGMVPLVPGGLAYQAVRN
                     LVTESYREAAHYAVQAIMIAGAIALGLVLSEVFNHNIRNFKVKRDAIMMKRKKRLFK"
     gene            complement(3175..3927)
                     /locus_tag="IV334_000174"
     CDS             complement(3175..3927)
                     /locus_tag="IV334_000174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HAP8942278.1"
                     /translation="MTGKELDLLLDTCLLAGKIMMESNAEMYRVEDTMSRIALASGNF
                     RLVSYVTQTGLFIGLDRTSTIRMEQITNRSINLEKVVKVNDLSRKYVASELTLEELYC
                     ELQAIDRDRSFFPVWLQIVSAAVISGTIMVLFGGKMTDLPVTLVIGGLGYAAYLYSLK
                     FFRIKFLSEFLSSLLIGIAAIYSVRFGLGSNQDLIIIGCVMPLVPGVQITNAIRDLLA
                     GHYVSGVSRGTEAMMTATMIGFAIAFIFQLFY"
     gene            4044..4114
                     /locus_tag="IV334_000175"
     tRNA            4044..4114
                     /locus_tag="IV334_000175"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4076..4078,aa:Gly,seq:ccc)
     gene            4475..5200
                     /locus_tag="IV334_000176"
     CDS             4475..5200
                     /locus_tag="IV334_000176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA non-specific endonuclease"
                     /protein_id="HAP8942279.1"
                     /translation="MSKKTTKAIVGIVAAVIAGAFGVSTTQKDSDIFQQITTIFDSTP
                     QASQVADVPEYDGKHQEIEINGNQPEFTKEDLSLSKGTWESYSNIDKLNRVGQANAML
                     GKDLFPTEKREPLYIDPTGWKQKKLSDGQWLYNRSHLIGFQLTGQNNNIKNLMTGTRS
                     LNAPYMLAHENDIAAYIKETNHHVRYRVTPHFEGNELVARGVQLEAESIEDKKIEFNV
                     FIYNVQDGYTINYETGQATKTKN"
     gene            5455..5721
                     /locus_tag="IV334_000177"
     CDS             5455..5721
                     /locus_tag="IV334_000177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IreB family regulatory phosphoprotein"
                     /protein_id="HAP8942280.1"
                     /translation="MGFTDETVRFGFDDSRKKEVSETLAIVYRALEEKGYNPINQIVG
                     YLLSGDPAYIPRYRDARNLIRRHERDEIMEVIVKDYLSNHGVNL"
     gene            5718..6149
                     /gene="ruvX"
                     /locus_tag="IV334_000178"
     CDS             5718..6149
                     /gene="ruvX"
                     /locus_tag="IV334_000178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HAP8942281.1"
                     /translation="MRVMGLDVGSKTVGVAISDPFGWTAQGVEIIRINEAEEEFGFDR
                     LKELVAQYEVDRFVVGLPKNMNNSIGPRAEASMSYGEKIKELFSLPVDYQDERLTTVQ
                     AERMLVEQANTSRAKRKKVIDKLAAVMILQNYLDGSGKQLF"
     gene            6174..6479
                     /locus_tag="IV334_000179"
     CDS             6174..6479
                     /locus_tag="IV334_000179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1292 domain-containing protein"
                     /protein_id="HAP8942282.1"
                     /translation="MTEHNHSHDHEGHEHITLVDDQGNETLYEILLTVDGQEEFGRNY
                     VLLYPAGVSEDEDVELQAYAYIENEDGTEGELEQIETDAEWDMIEEVFNTFMAEEEE"
     gene            complement(6560..7705)
                     /locus_tag="IV334_000180"
     CDS             complement(6560..7705)
                     /locus_tag="IV334_000180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAP8942283.1"
                     /translation="MATFKQYETKKGKFWLYEAYLGMNKMTGKPDKVRRRGYKTKKEA
                     QLALSRLQVDYDKNGIKKANNETFKEVYDLWLETYKTTVREATFMKTEIKFRKWILPK
                     YGNLRVNEVTVKKAQQIVNSWAETTDQYKVLHSTSKRIFSYAINLGIIETNPLDNVLM
                     PKRTTTKHKEKVKVYSKEQLITLFDYLNSKPETYRNDFDKTLLRFLFYSGVRISEALA
                     LNWSDIDFQENTVTINKTLSQSKNGYKISDPKTDCSAGVLPLDERTISILKKWQINQR
                     KYMLTLGVTDPTMIFCGIYKELITHHAIYARLITICEKAMVPFLGVHVARHTHASMLL
                     DAGATMVEVQTRLRHTKISQTIDCYGHLAKETKEKTVEKLVQHLNID"
     gene            complement(7766..8413)
                     /locus_tag="IV334_000181"
     CDS             complement(7766..8413)
                     /locus_tag="IV334_000181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP8942284.1"
                     /translation="MNGGENVARKKYPEAVETGQRIRLARIEKGLSMEQLGGKLSPPA
                     SKGAVSNWENGYNLPNNTRLKQLSKVLDVSTTYLLNGSYTLRDMHMMPEIKRNVLEKQ
                     LKKTIEKNNSIIDSNLSNAINNIEEDNLTVSQKIYLTNAIKYLKTGSRIEFPDGSTPI
                     AYLAALLNTLLRYDSLINDSEKNTDEKKEYQEFYENDLLKEFTALVTELNKHYSK"
     gene            8607..8900
                     /locus_tag="IV334_000182"
     CDS             8607..8900
                     /locus_tag="IV334_000182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8942285.1"
                     /translation="MELKIDKLTLDDPAFKIIDDRVVQQVEQTQGTLLSLLEKKYQFP
                     KYMNKAQAAKYMNVSYNTMMQKYVPNGLKLIIVDGVVRISQDECDRFMEEYQK"
     gene            8944..9255
                     /locus_tag="IV334_000183"
     CDS             8944..9255
                     /locus_tag="IV334_000183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8942286.1"
                     /translation="MESTQNKAIEKVLSTILTEDTAAELANLEGKALEETFECLYEQM
                     DYQKLLPQEPTVSGVLRGLNDLVQAEFKERLSIEEYQEILYQQVELLASLLGLELEDV
                     E"
     gene            9252..9876
                     /locus_tag="IV334_000184"
                     /pseudo
     CDS             9252..9876
                     /locus_tag="IV334_000184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350005.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage repressor protein"
     gene            10176..10538
                     /locus_tag="IV334_000185"
     CDS             10176..10538
                     /locus_tag="IV334_000185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8942287.1"
                     /translation="MYKTKLLNQLDSLELEEINQGIAELENNIGKTYFGNSFNEKLTV
                     LYVLKKHAEHKIICREINELKNQILTAWLNITDMQEARVKTFNTWVKYQNQLKGAEFV
                     RDGLKYELEQLKLMEVSE"
     gene            10575..11423
                     /locus_tag="IV334_000186"
     CDS             10575..11423
                     /locus_tag="IV334_000186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication protein"
                     /protein_id="HAP8942288.1"
                     /translation="MANNYQAATELQKSGWSIYPLSPGTKIPIKGSSGWNDATNDSNQ
                     INEWWTEDATRNIGLMLEDHQMVVVDLDQHSETRNGVENYKKLASMYDPFPSTYTEIT
                     PGKGIHFFFKIPDGVIIQQETGAFADIFGRDDKGKSLSGIDIITKGVPIAPTMIISRS
                     VGRAYEPIENVSLNNIAPAPDWLVNLLLKKPQEIKTQYRPVAKTGTAKKLDMIVQGAG
                     EGSRNDHLTKLCGWLLWHGVDNQTLAELIYTANAYNEPPLKDKEVNQIIRSMIKKDLR
                     GKAHGS"
     gene            11413..12888
                     /locus_tag="IV334_000187"
     CDS             11413..12888
                     /locus_tag="IV334_000187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase"
                     /protein_id="HAP8942289.1"
                     /translation="MAHKHKLDSILDFPEASEREDNIIELKTWMSRLRCNKDDQIKSN
                     SVVNAELILTNDSNLAGTIAYNEFSGYIHLLKDSPWINRTAGEWEDSFEDALTAYIEE
                     NYNVVFDDNKIHKAVVNVARKNVFNPVKERIEKVKWDQQPRLETLFIDLLGVDDNLYT
                     REVTKRWIVGSVARIYKPGIKFEIVPVLDGPQGIGKSTVPALLYTDDFFTDSLDSLGE
                     KKDDYMQLQGNVIIELGELSSMNKTKIEQIKNFISAKIDKIRPPYGRNVIAWARQCVF
                     IGTSNDGQYLKDDTGNRRFYPLPCKNKPKMNPFKTKDEYFLQVLAEAKVLFDKGQRIY
                     FSPDEDKEVLEIAKDYQEDAKMENPIKEAITKYLEMEIPYKWEEAPAWARRSYYQNYP
                     DSKCNEKNLQHFYNGATMQQEFYLVDSVLTADILEAVFDKQAKDLLNGRSDAETKKIA
                     LIISSIPGWERKQLPKRNKRRGFYNKSNAYENKNRAGKYKK"
     gene            13154..13561
                     /locus_tag="IV334_000188"
     CDS             13154..13561
                     /locus_tag="IV334_000188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3206 domain-containing protein"
                     /protein_id="HAP8942290.1"
                     /translation="MLNIISTNKAPNFQYTDEMDRFLMNTLAFSVGLVTEDYSTFDPE
                     VLKIMEEEPDWLQESVAWCQSLVVGSLVDSGNYDDTGELMDEFNCLLNLYDRARQREL
                     TSNEDNLFLNIHDKFLALLLTDDELITNLLEVE"
     gene            13564..13779
                     /locus_tag="IV334_000189"
     CDS             13564..13779
                     /locus_tag="IV334_000189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8942291.1"
                     /translation="MHMESIRYFDGEKHIEYSNTQEDVDFISFEEDKITKVNFKDGTC
                     LKIVSPYIEYKSKWEQDDSDERDRFGW"
     gene            13783..14163
                     /locus_tag="IV334_000190"
     CDS             13783..14163
                     /locus_tag="IV334_000190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HAP8942292.1"
                     /translation="MAVKKQCNHAGCKTLIDYRQKYCGKHKAKQTAIKREERKQSEGK
                     YFQFYQSRTWRKASYLYRLNHPVCEDCLEEGLIRKADVVDHKIELKDDWNRRLDETNF
                     RSLCHAHHNSKTANERQRREKSTL"
     gene            14297..14452
                     /locus_tag="IV334_000191"
     CDS             14297..14452
                     /locus_tag="IV334_000191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010780558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2292 domain-containing protein"
                     /protein_id="HAP8942293.1"
                     /translation="MKVGDLLKNTIVITDKTGKQRTIELPHFGQVTIQMQNGKIIYID
                     KLDKEKF"
     gene            14521..14994
                     /locus_tag="IV334_000192"
     CDS             14521..14994
                     /locus_tag="IV334_000192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase small subunit P27 family"
                     /protein_id="HAP8942294.1"
                     /translation="MGHPKILHDTKGNISSEEKAIRVDAREELFKQQPLINITPPDWM
                     AASARSEWNRIVPTLKKDYPLSEADYGSLVAYCLAFARMKTAEAEIRKSGTFITCENG
                     VKKANPAVRVQSQAMSDLKKQATSLGMTLESRSKLALNKVKNDEPEDPFKELMNS"
     gene            14991..16685
                     /locus_tag="IV334_000193"
     CDS             14991..16685
                     /locus_tag="IV334_000193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HAP8942295.1"
                     /translation="MNDYIEKVLSGKLIAPKKIIQACERHISDLERSKSDSFPYVFDE
                     EQATKAIKFIELLPSTDGKAIKMLGFQKFILGSLYGWRTKEGNYRRFNRAFTSMSRKN
                     GKTYIASGMAANALIMEQEPAEARQVLFVSNALKQAKLGYDMLSNSLRNVVKSSKFLR
                     PQLKIMNSKIQHLPSNSFAMALASETSTLDGFAPTTAILDEWHEAKTRKTYNVIKSGM
                     TQQKNGLLCVISTAGLDLNVPMYEEYLLLDRVLKGEEQADRYFIAIWELDDPEEIHDQ
                     EKWIKANPIFESEEIKKVMIPTIQDDVNLALKQNNLNSVLVKNFNLWRQASEDSYMIA
                     EDWQATEVEPQDITGKPVYIGVDLSKTDDLTSVSWIVPLDNGELYCDSHSFVATKYGL
                     QDKEKRDGLPYRELEKAGECSITQLESGIVDYDQVFQFIQDLIQENDLECMGICYDPY
                     NANSLISKAEKANYPMLEVRQGTITLNVPTRTFREQVYEGNVIHNKNTILTHAVNNAI
                     LKTDNNGIQINKSKNSNKIDPIAALINAYVFAMDYFTTTEGAKADNEFYTSEEFSF"
     gene            16651..16836
                     /locus_tag="IV334_000194"
     CDS             16651..16836
                     /locus_tag="IV334_000194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8942296.1"
                     /translation="MNFIQVKNFLSNYIHTVLLLLGLVCVLVAITLLTNVYYGLLALG
                     IVLIGVAVMLNTEQKGG"
     gene            16840..18015
                     /locus_tag="IV334_000195"
     CDS             16840..18015
                     /locus_tag="IV334_000195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HAP8942297.1"
                     /translation="MAFFKARQNTTGDPFLQHVVSIQSDDYTTSFTSVRALRNSDVFA
                     AVRIIASDIASSPIQLVKNNMPQADNELVKLLNEKPNSEMDGWHLKFALAVNMLLNGN
                     SFAEIKRNGEKVEEIHLLPNSSVTVTQLDNGTLSYQIGDKKRRVKSSDILHFKYFTQD
                     GLTGLPPLYALRDELKIQQAGNRTLHNFFTRGVSGSGILKVHKSDLDGSAKNAIREKF
                     EEANGSSSGDNALRTIILDETMDYKTLEINTDVLKLINSNDWNTKQIAKAFGVPIERL
                     GVENEHSSTVQSNLQYIQSTLIHYFNVFVSELDTKLETNIRFNSDQLLETDPETKVKN
                     VLDQVKGSLLTINEGRSKMGLPPMDGGDRLLASLNFTYLDTLEKYQLKEQEGVTPVE"
     gene            18008..19531
                     /locus_tag="IV334_000196"
     CDS             18008..19531
                     /locus_tag="IV334_000196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HAP8942298.1"
                     /translation="MNNEEEKEKRLTEEAELTADSPKVEKENEKQPTDGKTISGYALK
                     FGQPSKDLGGFVEVITPEALKEVDLSNVFLLYGHDYSKPLASVKAGTLKLNVDDTGLH
                     FEAELTDTTYSNDVYENISKGVVDSMSFGFVLGLDSFDKKEDGTIERSIDKIKALNEI
                     SVVTVPAYDSSNVQVNKRSYESFMSNNQAKQTNNSLESTSKAQKESNNMEKTLIDNEK
                     TELRGYEEYIRSQGEVRDGVTTVNAAAVVPEEVIGEVFDLKRSNYNLAQYATVKTVSN
                     GQGKYPVATNQQAVLATKAELAEIGDIDAEMFTSVDYKVETRAGKIALSNEVVEDSAV
                     NIVQEVKDQLAKLVENTDNKHIMDLLKTFTKKTAATLDDLKQLYNVALDPALNKMVIL
                     NQSGYYHLDTLKDSDGRYILQPDVTAPSGKSLFGMPVVLIADTLFANPKAGTFPMIMG
                     DIAQSIFVARRNQVTTQWEKFDYYSQGLAVIVRNDYKKIDQNASVYIEFTPVVTPKV"
     gene            19587..19871
                     /locus_tag="IV334_000197"
     CDS             19587..19871
                     /locus_tag="IV334_000197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage gp6-like head-tail connector protein"
                     /protein_id="HAP8942299.1"
                     /translation="MVILDSIKKSMRIDHTIDDDFIQQLIDTAGEYIKSAIDSSATDK
                     DMDNYQQFDLAVSLLTQHWYLNRQEASSERIPVTVQALVQQMRGAYYANH"
     gene            19858..20193
                     /locus_tag="IV334_000198"
     CDS             19858..20193
                     /locus_tag="IV334_000198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HAP8942300.1"
                     /translation="MPIIKNVNELTERVKFKKTKRVKDEDGQMVDSEETVFECWANVR
                     SQMLKDVLASVGTILEGTLTFIIRYDQDYELTNDMKVAWKNKSYKIISINEGTAFKDY
                     TTIIAKQIS"
     gene            20488..21114
                     /locus_tag="IV334_000199"
     CDS             20488..21114
                     /locus_tag="IV334_000199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8942301.1"
                     /translation="MDKAEFTNFYWKYYLHLENEFINTTDFVMLDENNFKTFSIEYQK
                     LLLAIGSECEIIFKELCGFDSSSNKSITDFKAVIKSSELMCLDNGVRVMNSLTLTSLK
                     PFGDGWPEDTPDWWKIYNRVKHGRSSNYKEANLENVLYALASLYLLEEYLHKKVILEG
                     EVDFISPESNLFTLSWERKHSSMQKVTFEKVDSNYVAPLIDFKEQEEI"
     gene            21856..22693
                     /locus_tag="IV334_000200"
                     /pseudo
     CDS             21856..22693
                     /locus_tag="IV334_000200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325086.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
     gene            22758..23192
                     /locus_tag="IV334_000201"
     CDS             22758..23192
                     /locus_tag="IV334_000201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP8942302.1"
                     /translation="MLREMRLEDAEALREINADQLGYDIPLTVTVRQMKKLLKEPEKN
                     FFLVYEDPTSKKAAGYVQAESYESIFSDPMFNIMALAVSKYAEKRGIGKALMTGVEEE
                     AKKRGITTIRLNSAEYRVEAHQFYEHIGYHSDKMQKRFLKIL"
     gene            complement(23260..24582)
                     /locus_tag="IV334_000202"
     CDS             complement(23260..24582)
                     /locus_tag="IV334_000202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxycarboxylate transporter family protein"
                     /protein_id="HAP8942303.1"
                     /translation="MAQKTTTATAVEEKKKEGFWSTKITGVSLPLYLIMVLVLIIAMA
                     LGKLPTNMIGPIAVLVIFGNLFHFIGTKIPIVKSYLGGGSVFAIFASAAIATFGILPE
                     YVVKSTENFVSNMGFWDFYIAALITGSILGMNRNLLMKASVRFIPVALISMVVSFFAV
                     GLVGMLIGNGFANSVLYISLPAMAGGVGAGAVPLSTIYANVLGTDASSIISRLIPATA
                     MTNVLAIIGAALVARAGQSMPKFNGNGKLMEKGDLGDGKPRTVKPSIPQLGVGLMVSL
                     TFFILGQIGNYFVPKVHAYAFMIIIVVICKIANLLPEYYEDAAIMFNNLIVKNLTAAV
                     LAGIGIALLNLNVLASALTWQFVVLCLTSVIAISVVSGFVGRLFGLYPIESTITAGLC
                     NNSMGGTGNVAVLSAANRMELIAFAQMGNRLGGAIVLIISGFLMQLLS"
     gene            complement(24736..26376)
                     /locus_tag="IV334_000203"
     CDS             complement(24736..26376)
                     /locus_tag="IV334_000203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent malic enzyme"
                     /protein_id="HAP8942304.1"
                     /translation="MLTGLNLLNNPFLNKGTAFTKEERAKYGITGMLPSTVQTLEQQS
                     VQAYGQYLSKQTDLEKRIFLMNLFNTNRTLFYKLMGEHLVEFMPVVYDPVVADSIEQY
                     NEIFLKPQDAAFLSIDEPENIKASLKNAADGRDIRLIVVTDAEGILGMGDWGVNGVDI
                     AIGKLMVYTAAAGINPAQVLPVSIDAGTNNEELLNNPLYLGNRHARVEGETYYEFIDQ
                     FVQSATELFPELLLHWEDFGRGNAAAILEKYEDQITTFNDDIQGTGIVVLAGVLGGLN
                     ISGEQLKDQIILTFGAGTAGVGIANILLDEMIRQGVPEEEARQHFYQVDKQGLLFEDT
                     EGLTPGQIPFARKRSDFTNADELTNLEAVVKAIHPTIMIGTSTQPGAFTEEIVKEMAA
                     HTPRPIIMPLSNPTKLAEAKAKDLIEWTDGKALVGTGIPAADVEYNGVTYQIGQANNA
                     LMYPGLGLGLIASTATRVNAEIISQASRALGGIVDVTKPGAAILPPVAKITEFSQTIA
                     ETVAKSVVAQNLNREEITDIKEAVESAKWVPEYKSLED"
     gene            26706..27176
                     /locus_tag="IV334_000204"
     CDS             26706..27176
                     /locus_tag="IV334_000204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4809 family protein"
                     /protein_id="HAP8942305.1"
                     /translation="MKEVQLIRKSELSEGGCNACGVVEATSYTLKLDSNQAIISELTV
                     GGLVDSLALAEGFTGEDIYEMFSEVRQLKKGEKCIEVHHESPNVRFKRGDNEMIFKNH
                     VSDHTELYEIVNQILSGIFELGPYEFKEENGNPKLNEEWQETIETQRNNPHLFQ"
     gene            27805..28908
                     /locus_tag="IV334_000205"
     CDS             27805..28908
                     /locus_tag="IV334_000205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate oxidase"
                     /protein_id="HAP8942306.1"
                     /translation="MEKTYQAGTNEGIVDFINMEDLEIAASQVIPAGGYGYISSGAGD
                     LFTYQENERAFNHRLIIPHVLRDVELPDTTTHFDEEMLTAPIIMAPVAAHGLAHVKAE
                     KASAKGVADFGTIYTASSYASCTLEEIREAGGEKAPQWFQFYMSKDNGINLDILEVAK
                     RNGAKAIVLTADATVGGNRETDRRNGFTFPLPMPIVQAYQSGVGQTMDAVYKSSKQKL
                     SPKDVEFIAAHSDLPVYVKGVQSEEDVYRSLESGAGGIWVSNHGGRQLDGGPAAFDSL
                     QYVAEAVDKRVPIVFDSGVRRGQHVFKAIASGADLVAIGRPVIYGLSLGGSTGVHQVF
                     DFFKTELEMVMQLAGTQTVEDIKKIKLRENRFI"
     gene            29192..29872
                     /locus_tag="IV334_000206"
     CDS             29192..29872
                     /locus_tag="IV334_000206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HAP8942307.1"
                     /translation="MKKNMIGTLLLSTLLLGGLATPAFAEGQATSKGDITFTEPTNTV
                     EPLNPTDPSKPVEPADPENPATGQTGSLTLDVVPELPFGTHEIESGTKTYQVDASKND
                     TPYLQVSDRRGVGADGQAQGWNVTVSVSDFVNGSQVLQGAELDFGTSTVKSTSDNEST
                     GPTSQTVTGLSKASAATPIFTAAKDQGLGTWLSVYDPANITLKVPKAAAGTFTADLTW
                     NLVAGPVA"
     gene            29962..30996
                     /locus_tag="IV334_000207"
     CDS             29962..30996
                     /locus_tag="IV334_000207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 and DUF3324 domain-containing protein"
                     /protein_id="HAP8942308.1"
                     /translation="MNQSKRLFVSFFSILSLFFIFPSISQAKDSAGDFGIKPVFPENQ
                     IDKAIGYFDLLVAPEQNQTLEVIISNSSDEERTFEVSVNPAVTSDGGTIDYSQKNPTL
                     DETLPFDVRDVLLIAKKEINVSAHAETTIPIEVKIPAKSFKGRVLAGIHVSPKEEAET
                     ENATEGAQIKNRIAYNLAVVLQESQETIEPDLKLLSGDLDEVNAKPTVQLRFQNPQPR
                     IISNLIFTSKIFYENQLYIENTSNEFLVAPNSNFHLNLDLAGDKAKAGDYRAEIIAKS
                     GDSNEWRFTQNFTIKKEKAQKVNENSVFAVQEQSFPWLYAGLVAAVLVLWLILLFLWV
                     HKKKKKGETT"
     gene            31010..31345
                     /locus_tag="IV334_000208"
     CDS             31010..31345
                     /locus_tag="IV334_000208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8942309.1"
                     /translation="MKTSILLGGIIASLLFFSWQEPVQADTKSSGTITFYDLSPVPHP
                     SGSVNGASKQQTQLPIYKESNQHGVFPETNEEKIRFLYLIIGIELWLLAWLLLWRRKE
                     KEDEHVATK"
     gene            31329..33809
                     /locus_tag="IV334_000209"
     CDS             31329..33809
                     /locus_tag="IV334_000209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8942310.1"
                     /translation="MLLQNKFSFFRSFFFTSLFVLSIFIFSPSAEAVGATPPAISYDP
                     YTYENIVPPNGGRSAVNGGKLVQLTGANQLPKLTATYLNDGSNDPWKGGTVRFHWESH
                     VKLTNVLDLVNITAQGFAFVAIINLPEHVSSSDVLKAIDWNNAYLYIAGAPIKVTSGS
                     LLSAGSHTLRFGMGSWDTNTLLADIIAIITRGEFNVDDIPLQFDVTANVAKMTENGDK
                     YDTSPNKILTKGKLQPSMSKKAVFSVDFYDSNNIIQDRMFNSILAPAAGRFYPKLTGG
                     NLNPGAYAFSASAEINTWNSYISTSDSTGKYNTFADTTEYVDGNNQNLPGSTNSRTLT
                     MGLTEGRFDQLPFSRFDRVVNYFTGKNVTQGAILTHTPASTSGLNQTANIVYSGKDAQ
                     GGLLSPVALKVLEQYKLDGTVLPTNLTADSQWGKLLSSASYQVTANWSATELKKGSIH
                     YRLYSKGTQKLATGWTDQLFQTIQSNNGGVNTATSTLPALASGQYYFDYRLVDDVLTN
                     MYPSFAYKWQSEQAGITTLPQITVANFPSVSADSQLKNLSRQPETGDPLSALSGDTIQ
                     ENVTFTLTKSGDAITDKKITISLPENTTYINGSLKLNGTTIADTGIQQGVSVPADLLS
                     KIGDTIHLTYNYQLNTVDTSVQSVSILTKAAVLSSNITLADGAKLPNPVVQTSAKTIL
                     VPKQELTLVNVPDDFTFGNDLPKPLKTSYYEAKGDFSFDVRDTRLPSTSPWQLTGTLT
                     SLFKNDQGQELSGTKLYFNHSGSKQLIQQGQNTLIYESDGTAKGEVLVDFPDTDGLLL
                     EVNSSTNAQPGATYQGMVTWELTAGPTS"
     gene            33865..35424
                     /locus_tag="IV334_000210"
     CDS             33865..35424
                     /locus_tag="IV334_000210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP8942311.1"
                     /translation="MRRKLLLLKSEMLGGVDLELFSLLEKNEQRQIKLFQLLLEEKGE
                     ISVNNVLTRLSVDRATLKEDLFYLQANLENIKQLEIVIQEGNILLIRHGNIAAVDVYY
                     HCYLRYSTKYQILLYLLENGSFDRQKLVMELNISSATLSRRVKELNDSLNEFHLKIKS
                     GRLTGPESQICYFYFQLFWFGRPYLENKKEYSDLSGNTFLDLLQKEFSFTITEEGRLK
                     FEIWLSIMKKRMRCTKKSKPDYSNLPVGLFQTTQFLLDLHTFLTRYFFQYAFPWNQNE
                     TLVFYLFMISNFTLDTHNSHVQEFLISEKYKDDVVAQLNRTFKQSIEEYFSFYVFPEE
                     ISQKIALTVMQNHAHFAYFRGWMNIFGQEGLKQRLTEVEDKQFLVLCERLVRQTFAIV
                     DFTEVDEENARFELFARYASILRTLIKQLELRLQIAYDLPYERLMADIVKEIIQEQLL
                     NTIKYDLVEYEKEKQYDIILTSQLKEYPSQKSAKVFVFTSNEYNYDFPYLNQFLKECY
                     LEKLNLRMNKT"
     gene            35421..36785
                     /locus_tag="IV334_000211"
     CDS             35421..36785
                     /locus_tag="IV334_000211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HAP8942312.1"
                     /translation="MKIVIIGASHAGITAALNLRKLQPKAEVLLIDEDHKDGLGYVSN
                     GINLYLKGKIRSLAEVAHNMRTLQESGAKLITEWRVTELDPDKHQLILASKEGKNETI
                     TYDKLIVATGSSPVTLYKQIEAENVYTYKNLVQSKQVLAALKEAKEVVIFGAGYIGLE
                     LADALRNKGYIIHLVDYMPNVLSRYFDKDMINSFQNQLQTKQINFYPNEFLIDWKKSE
                     EKVVSVQLLSQAIKADMVIFSAQTRPNTTLLKEKVALYEDETVIVNEYLQTSDPDIYA
                     IGDIVPVSFDKNKRHLFLPLVTRAVHMARAVALTLSGQPTAYDLRQKITAAVIIDYFL
                     GTVGLTEDEAPFLEQNTGSCSGEFDLFPQYDEENKTVNAKLIYHPDTLEIIGGQLISQ
                     EFLLSDLNLLADIVKHKTTIPQLAVEDFGFLSEHTSRFHYLNELAFKVLAKRANSNRV
                     DKRL"
     gene            36956..38026
                     /locus_tag="IV334_000212"
     CDS             36956..38026
                     /locus_tag="IV334_000212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP8942313.1"
                     /translation="MFLALNEIMHSKLRYALVAGVMFLIAYLVFFLTGLAYGLAQDNR
                     TAVDKWEADSIVLSKDANSNLGMSMITKKIAEEVEGGKVAYLAQTPGVVTSKDSTEEG
                     KINVSFFGIDKNQFIMPNLVEGKAFDNDDEAVGDISLKEEYGLAVGDTVKLSGSDKTF
                     KLTGFTDHAKFNVSPVLYTTINAYQEIRFEKEDTSENARINAIVVRGKISNLPEDLEQ
                     IKISKFINELPGYNAQVLTFGFMIGFLIVIAAIVIGIFIYVLTMQKINIFGVMKAQGI
                     TGGFIARSVVAQTFILSFVGILLGLLGTVGTSLVLPDAVPFQSNWLFFGVISLLMLVV
                     AVLGALFSVRTIVKIDPLKAIG"
     gene            38041..38709
                     /locus_tag="IV334_000213"
     CDS             38041..38709
                     /locus_tag="IV334_000213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP8942314.1"
                     /translation="MKAIEFKEVEKSFQDGDQVVKALKETTMSIDKGEFVAIIGPSGS
                     GKSTFLTLAGGLQTPSSGSVFINDQEFSNQKESERVKLRFKEIGFILQASNLVPFLTV
                     KKQLLLVDKIKHEHRQKEAKELFEQLGVDKLINKYPEELSGGERQRVAIARALYNDPS
                     IILADEPTASLDSEKAREVVDILAKESKDKNKATIMVTHDTRLIDKCDRIFMMEDGVL
                     KEKE"
     gene            38729..39307
                     /locus_tag="IV334_000214"
     CDS             38729..39307
                     /locus_tag="IV334_000214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAP8942315.1"
                     /translation="MVHRYNKDTQQKILKEATDLFMSKGYLGTSTREIAQKVGITQPN
                     LYHYFGDKEKLYTAVLENHLKDVGKALREIVQTSETGFQVTLTKMAQFLIETHLVDLF
                     LMLHDLESNLSKETRDHLFFLWKKNYREPFEEIFSANQSVLRNGISKEIAARHFFLVL
                     APYITRSKRPVEEPLNVAQLIDLYLNGVLGEA"
     gene            39710..40621
                     /locus_tag="IV334_000215"
     CDS             39710..40621
                     /locus_tag="IV334_000215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HAP8942316.1"
                     /translation="MKKIIGAFVFAIIIIIICFQIDVFGQKKIVGQTVSSTNSTVTKT
                     KESFDDTEQETLIKIGKNDRTLYTDDSLTTPLATINGGELAEFLSETKNAYQIKTNDG
                     YTGYLALEDGTKVTKNIQTKPEELSDAVIVLDPGHGGNDTGALSNDEQTEEKDITLST
                     AIKVKKALEDAGATVYLTHTTDELVQLGDICDYSEEKKADVFISLHADSTEYANEATG
                     ITTYYYYGQEETLAQTIADSFTDLPLDSRGISTGNYQVLRENLQPSILIEMGYMNNDS
                     DLKELVTDSYQQQIAASLTQALTTYFQ"
     gene            complement(40877..41794)
                     /locus_tag="IV334_000216"
     CDS             complement(40877..41794)
                     /locus_tag="IV334_000216"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HIII"
                     /protein_id="HAP8942317.1"
                     /translation="MSNVIIKATNDQLSQMKAYYRSYLLNKQIPYTSFAAKKDGTTIT
                     AYTSGKVMFQGTNAAIEADKWGEAQDKQAKPAKTASSLPKNISQLSVLGSDEVGNGSY
                     FGPLTVCAAYVKRDQLAELRKLGVRDSKELKDSQIIQLADILKKTIPYKLLVLSPEKY
                     NEIQPKYNAVRMKVALHNQAIHLLLKKIEPEKPEAILIDQFTPEANFKKYARLEKNQL
                     QEKLYFVTKGEQYHLAVAAASIISRASFLEELHKASAEAGITLPSGAGTKSDQVAAKL
                     LEKGGMPMLEKYAKLHFANTEKALKLLKK"
     gene            41996..42433
                     /gene="zapA"
                     /locus_tag="IV334_000217"
     CDS             41996..42433
                     /gene="zapA"
                     /locus_tag="IV334_000217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HAP8942318.1"
                     /translation="MAHEKTRYKAVIANQTYTIIGRETKHHMDIVTKLINEQLAELKQ
                     LSPQMDNEQAAILMAVNALSDQLKKQERILELEEETAELKKKMIKFTELENRVKRIEA
                     IENEAREVLKENGQADHTIKNHVEAQQILNEKRKGQIKQKSSN"
     gene            42491..43038
                     /locus_tag="IV334_000218"
                     /pseudo
     CDS             42491..43038
                     /locus_tag="IV334_000218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629130.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
     gene            43153..45513
                     /locus_tag="IV334_000219"
     CDS             43153..45513
                     /locus_tag="IV334_000219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HAP8942319.1"
                     /translation="MNNRILETLEFEKVKQMVRQFVVTAQGKEELAELVPVSEKQTIT
                     NWLQETEDGLKVQRLRGGIPIPKLENIRPHMKRIEIGADLNGVELAQVSRVLSTTSEL
                     NRFIDDLSDSEIEFARLYMWADQLVTIPVLSRRLKEAIDEDGRVTDDASPELKSIRQN
                     IRRSEQAVREQLDGIVRGKNAKYLSDAIITMRNDRYVIPVKQEYRGVFGGVVHDQSAS
                     GQTLFIEPKQVVDLNNRLRQYQIAERNEIQRILSELSAELVPHRQEIIHNAYVIGKMD
                     LMNAKARFGKEVKAIVPGISEDNHVVLKQARHPLIDQEKVVSNDITIGKDYQAIVITG
                     PNTGGKTITLKTLGLLQLMGQAGLPIPAGEESQIGIFEEVFADIGDEQSIEQSLSTFS
                     SHMTNTVDILSKVNEKSLVLFDELGAGTDPQEGAALAIAILDDLGKKSAYVMATTHYP
                     ELKVYGYNRANTINASMEFDVDTLSPTYRLLIGVPGRSNAFEISSRLGLDTEVIDEAK
                     QLMNDESQDLNEMITDLENRRKMAETEYLEMRHFVSEAQELHDDLKEAYSYFFEEREK
                     EMEKAKKKANEVVSEAEEKAEKIIADIRKMQQQIGQGNVKEHQLIDAKTQLANLHQEE
                     TLKKNKVLKKAKEQKTLKPGDEVLVTTYGQRGTLLRKNGNQWQVEIGILKMNVSEDEL
                     TPVAPQKEPTQRVIHAVRSESSSHVPNQLDLRGKRYEEALSEVDQYLDSAILAGYPQV
                     TIVHGKGTGALRKGITDYLKNHRSVKSFEFAPANQGGNGATIVKFK"
     gene            45600..45914
                     /gene="trxA"
                     /locus_tag="IV334_000220"
     CDS             45600..45914
                     /gene="trxA"
                     /locus_tag="IV334_000220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HAP8942320.1"
                     /translation="MSQVITDKTFNEETDKGLVLIDFWATWCGPCRMQAPILDQLEQE
                     YDEEEFRIAKMDVDENPETPQQFGIMSIPTLMLKKDGQVVEKAVGVHSKEQLRQMIDQ
                     YL"
     gene            46096..47874
                     /gene="uvrC"
                     /locus_tag="IV334_000221"
     CDS             46096..47874
                     /gene="uvrC"
                     /locus_tag="IV334_000221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HAP8942321.1"
                     /translation="MNERIKNKLALLPDQPGCYLMKDKNGTIIYVGKAKILKNRVRSY
                     FTGSHNTKTERLVSEIEDFEYIVTESNIEALLLEINLIKKNDPKYNIMLKDDKTYPFL
                     KITNEKYPRLVITRKVLKDKALYFGPYPDVGAANETKKILDRLFPLRKCKPSQTKEPC
                     LYYHLGQCLCPYYFDVDPKVYTKIVDEVKKFLNGGHEKIESEIHQKMEKAAENMEFEK
                     AAEYRDQIRAIETIMTRQKMTNTDLLDRDVFGYAIDKGWMCVQVFFVRQGKLIERDVS
                     MFPFYNEAEEDFLTYLGQFYQENEHFIPKEVLIPDTIDKPSVEALLATKVLQPKRGEK
                     KKLVELANKNASVALNERFDLIARKQERTIGAVERLGNAMNIPTPIRIESFDNSNIMG
                     TNPVAAMVVFIDGKPARKEYRKYKIKTVNGPDDYASMREVIYRRYSRVIREGLPLPDL
                     ILIDGGKGQVDVAKDVLENQLGIDIPIAGMAKNDKHKTNELLFGIDLAVVPLERNSPE
                     FFLLQRIQDEVHRFAITFHRQLRSKNSFASRLDEIEGLGPKRKKMLLKEFKSLKNITA
                     ATTEELQEIGLPKNVAQNVFDKLHQE"
     gene            48019..48918
                     /locus_tag="IV334_000222"
     CDS             48019..48918
                     /locus_tag="IV334_000222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP8942322.1"
                     /translation="MLEVKDLVKTFGNYTAVDHVSFQIPDGKIMGLIGQNGAGKTTTF
                     RLILDFLTQDQGEVLWNGHHLGEKDYDVIGYLPEERGLYPKVTIQDQLLYFAELRGKS
                     RKEIEPKIDFWMEKFQVKGKKTDKVKSLSKGNQQKVQLIATLIHEPKLIILDEPFSGL
                     DPVNAELLKDGILELKKHGSCVIFSSHNMDNVEKICDHLIMLRNGKMVLNGKVHEIRE
                     SFGRTKLFLESGLSQQEVEEIEGVKKVILHEDNTLEITLEDPEAGKEIFTRATQFGYI
                     PMFNQQPPTLEEIFKLKAGEIDA"
     gene            48911..50173
                     /locus_tag="IV334_000223"
     CDS             48911..50173
                     /locus_tag="IV334_000223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP8942323.1"
                     /translation="MRKFWIITKDVYLKNVKSISFLIMILVPFIMLGIIYVAGNFAQQ
                     NSDVNKIGVLADNEQISQQMSQVKTDDFEFKAVDSEEKAQKELSEEKIDAYLVVSTEN
                     DKINGMLYSENSLGQSTQLLIQQQLTGLQSMLRASQLGISPEEVASLSQQASLTRQKV
                     NFDSKGKMTVGEDNSTVQYVVSYVATIVLFIIILTYAQIIAQEIASEKGTRIMEVILS
                     STTAQKHFYGKLTGVLLVALTQMALYGIIFGIGYSQFKDMEMVKNILDGISLDNLIGP
                     FLWYSLLFMIFGILIFSVLAALCGSLVNKAEDTAKAILPVTYLSLGGYMLGLILGASD
                     PNNIVIRITSYIPFLSSYIMPVRLANETVGMGGALISLLVLVAVTFLLMILSANMYKS
                     NVLVYSEGGLWSSLKQSISIMRNERKKA"
     gene            50336..52234
                     /locus_tag="IV334_000224"
     CDS             50336..52234
                     /locus_tag="IV334_000224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphatase class III"
                     /protein_id="HAP8942324.1"
                     /translation="MDRYLDLLSEKFPTTEDVITEIINLEAILQLPKGTELFLSDIHG
                     EFPAFDHILRIGSGNLKEKIKDLFSEQMTETEISQFTIFTAYPEYVLTTDWYKKQDKS
                     QLIHRLIDLLRFTTVKYTRSKVRKGLPKEYSYIIEELLYIDDRINGKKDYVKKIIEQL
                     VLMKEEEKFLTKLAQTIQKSVIDHLHIVGDIFDRGTQAAKVMDRIMDFSSVDIQWGNH
                     DILWIGAYCGSEACLLTLLRIAARYNYLFELEKEYGLNLRPLFSFAHQTYQKNPVFTP
                     KNRKDLSEREQEELEKIHQALSILQFKSEHQLLNRRPEFKMDDRKLLEKINYEESTIV
                     LEGQLYELKGTCFQTIQPDDPKKFLEEEQKVIDSLMYSFQHSLRMQKHMTFLIEKGGM
                     YLTNNQHVLFHGCIPVDEKGQLLAFELDQSYRGKELLGFFEKQITESAKDLTANEDLA
                     TDLIWYAWSGPFSPLFGKSKMATFERYFLTEPHTHVEKSNPYYHLRDEEWFSEKILAE
                     FDAKEEGSAIINGHTPVKVKKGESPIKANGKAFVIDGGLSKAYQKTTGIAGYSLLCNS
                     YGFQIVTHYPFLPIEQQFAKKSDQTNVKKVIEQQLPRKLNRNTTKGMELQQQITQLQR
                     LLDYRKDD"
     gene            complement(52533..53252)
                     /locus_tag="IV334_000225"
     CDS             complement(52533..53252)
                     /locus_tag="IV334_000225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HAP8942325.1"
                     /translation="MKPIIGIAGNQLIRATDTFQGNQVSYTPQGFVDAVLDAQGLPLI
                     LPVMSPDSAPQLIEKIDKLILAGGQDVSPQLYMEDPHPKLTETNIQRDQFEQALILEA
                     LKQRKPIFAVCRGLQLLNVVLEGTLYQDLSLYPKWSVKHEQQPTAPQFATHEVKIVSD
                     SLLSDLLPDSYFVNSYHHQAVKDLSPLLKAIAFSNDGLIEAVQSKDNMHKILGVQWHP
                     ELSHRVDSSEQRLFDFFVNEF"
     gene            53589..53921
                     /locus_tag="IV334_000226"
     CDS             53589..53921
                     /locus_tag="IV334_000226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8942326.1"
                     /translation="MKQVLEALEQIRKAEEENEKRARTLRQQLKTYAEEKEQELEIIQ
                     TNSRQQLKQLVEEKEQFEKQELEKEKMKLKAEADKVQATLKDQYQKHQSQAIGAIIER
                     VKETYGRH"
     gene            53908..55911
                     /locus_tag="IV334_000227"
     CDS             53908..55911
                     /locus_tag="IV334_000227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit I"
                     /protein_id="HAP8942327.1"
                     /translation="MAVTKMEKVTLISDKRNQERILQAVQGIQNVEIRDLYQNRTNNQ
                     WVEKYFPDTTIIDKEAKLSALGNRLKDIQEAIQFIQHHGDSRQKKLHLKRQELSLQDL
                     EANYSEENFMNKLEEVLDLKEKWEQLTEKRDRLEEEEEWLLHWQQLDIAPSMYASKTT
                     IFEMGTVSAANFESFKEELSKLDDVYMEEIDYTPKQVYLSYVALKKSVAAIEEIAGKY
                     GFNKETYPYEQNPKEKLQETKQQLSIVHEQQKKITTALGQCSGYIQEFEWVEEVTLAV
                     AEREKVKERFIHATYLIVLQGWVDAEEKQNLMHVLNETVSEEDIYVSFETPNSEEIQQ
                     EVPTKLKNHPLIEPFELLTEMYSLPKYEEVDPTPWMTPFYLVFFGMMVADVGYGLLML
                     IGTILAQKLFVLPRGMKRFVKFFEILSVPSIIWGLIYSSFFGQALPKELFGIRLPFPI
                     LSTTDDVNTILILSVIFGLIQILVGLFVAAKENIRRKDYLSAVSDGFAWQGILIGIVI
                     ALIGVMLIKNTAFVYLGAGLSIIAALCILIVPIIQSPSKVKGAAKGAYNLYGLTGYIG
                     DLVSYTRLMALGISGGSIGAAFNMLVAFMPPAARFSVGILLIIALHALNMFLTLLSAY
                     VHGARLQYVEFFGKFYTGGGRAFDPLKTAEKYVNINHKKKNNK"
     gene            55930..56400
                     /locus_tag="IV334_000228"
     CDS             55930..56400
                     /locus_tag="IV334_000228"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit K"
                     /protein_id="HAP8942328.1"
                     /translation="MMDYLISQNGGMVFAVLAMATATIFSGIGSAKGVGMTGEAAAAL
                     TTSQPEKFGQALILQLLPGTQGLYGFVIAFLIFINLGNDMSVVQGLNFLGASLPIAFT
                     GLFSGIAQGKVAAAGIQILAKKPEHATKGIIFAAMVETYAILGFVISFLLVLNA"
     gene            56421..57005
                     /locus_tag="IV334_000229"
     CDS             56421..57005
                     /locus_tag="IV334_000229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase V"
                     /protein_id="HAP8942329.1"
                     /translation="MNAIEKIISQMNEAAEQERAALEQEERMKIDQNFEQKRTQIETE
                     HQKQKEKQIELLEKKYRQLRNRQQVEVRQENLNAKQEFLRRLFADAIAEMENWDESEQ
                     IQFIKNSLYSLPLTGKVTFIAGEKSAAYLSQTLLDEWNNELPFMMVLSDETVADQAGF
                     LINDQGVQYNFLFSSLVQDIQGTMSFEIANQLFE"
     gene            57020..58006
                     /locus_tag="IV334_000230"
     CDS             57020..58006
                     /locus_tag="IV334_000230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATPase subunit"
                     /protein_id="HAP8942330.1"
                     /translation="MEYHELNPLIRGRELELLSNETFERMIQTNSVEALGEILKSTIY
                     SPYIYDGYEYDFEQNLLKEQSRLFRWLKESAPEPEIVWIYTMRYTFHNLKVLTKAEMT
                     NQNLDHLYIDDGFYSLETLKDAIHTQVSNELPESIMDSIREVRDYFEESTILQGIDVI
                     YDRQFLTEQRRLADKLGYQELLTEVIAFIDLTNITTMARGILQKRTYGFMTTVLSSSG
                     SIEKEVFLNFVDKDMEDYTNFLLETDYADFLRPVIREGNLDLVKLEQLKDDYLSSLYQ
                     EAQTQAFGPLPLLAFLNAKEVESKNLRLLVIGKRSHFEPETIRKRVRQVYDA"
     gene            57996..58307
                     /locus_tag="IV334_000231"
     CDS             57996..58307
                     /locus_tag="IV334_000231"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007209277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit F"
                     /protein_id="HAP8942331.1"
                     /translation="MTHKIGVVGDKDSVLPFKLFGFDVRYGTTKQTVRQAIEEMAKNE
                     YGVIYVTEQCAALSPETIERYKEQMIPAIVLIPNHQGSLGIGLEEIQKNVEKAVGQNI
                     L"
     gene            58329..60110
                     /locus_tag="IV334_000232"
     CDS             58329..60110
                     /locus_tag="IV334_000232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit A"
                     /protein_id="HAP8942332.1"
                     /translation="MQIGKIIKVSGPLVMAENMSDASIQDMCLVGDLGVIGEIIEMRG
                     DVASIQVYEETSGIGPGEPVRSTGEALSVELGPGIISQMFDGIQRPLDTFMEITQSNF
                     LGRGVQLPALDHEKKWWFEPTVEAGETVSAGDVIGIVEETKVIKHKIMVPNGIKGTIK
                     TIEKGSFTIDETVCVIETENGDKNLTMLQKWPVRRSRPIKEKLNPDAPMITGQRVIDT
                     FFPVTKGGAAAVPGPFGAGKTVVQHQIAKWADVDMVVYVGCGERGNEMTDVLNEFPEL
                     IDPNTGESLMERTVLIANTSNMPVAAREASIYTGITIAEYFRDMGYDVAIMADSTSRW
                     AEALREMSGRLEEMPGDEGYPAYLGSRLAEYYERSGRVIALGSEGREGSITAISAVSP
                     SGGDISEPVTQNTLRVVKVFWGLDSSLAQKRHFPSIDWIQSYSLYSTEVGKYMDHILQ
                     ADWASMVTEGMRILQEEQQLNEIVRLVGIDSLSDNDRLTLEVAKSIREDYLQQNAFDD
                     VDTFTSREKQFKMLKMILTFGEEGRRALSLGAYLSEIMDGTVSIRERIGRSKYIPETE
                     LEKLDQIIGEIKQTMQTIVSEGGMSND"
     gene            60103..61479
                     /locus_tag="IV334_000233"
     CDS             60103..61479
                     /locus_tag="IV334_000233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit B"
                     /protein_id="HAP8942333.1"
                     /translation="MIKEYRTIGEVVGPLMAVEKVAGVKYEELIEVRMQNGEIRRGQV
                     LEVQEDKAMVQIFEGTSGINLRDSSVRFLGHPLELGVSEDMIGRVFDGLGRPKDNGPE
                     ILPEKHLDINGEVINPVARDYPDEFIQTGISAIDHLNTLVRGQKLPVFSGSGLPHKEL
                     AAQIARQATVLDSSDDFAVVFAAIGITFEEAEFFMEDFRQTGAIDRSVMFMNLANDPA
                     IERIATPRMALTAAEYLAYEKGMHVLVIMTDMTNYAEALREISAARREVPGRRGYPGY
                     LYTNLATLFERAGRIRGLKGSVTQIPILTMPEDDKTHPIPDLTGYITEGQIILTRELY
                     KSGIQPPIDVLPSLSRLKDKGTGPGKTREDHAATMNQLFAAYAQGKQAKELAVVLGES
                     ALSDVDKIYAKFAERFEKEYVNQGFYTNRSITETLDLGWELLSMLPRTELKRIKDDML
                     DKYLPEGK"
     gene            61486..62121
                     /locus_tag="IV334_000234"
     CDS             61486..62121
                     /locus_tag="IV334_000234"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit D"
                     /protein_id="HAP8942334.1"
                     /translation="MARLNVNPTRMELTRLKKQLTTATRGHKLLKDKQDELMRQFILL
                     IRKNNQLRQEMEESMTEAMSDFVLANASITEPFIEELFVLPAENVDLSIVDKNIMSVK
                     VPVMNFEYDEQLTDTPLEYGYLNSSAELDRSIDRFTTLLPKLLELTEIEKTCQLMASE
                     IEKTRRRVNALEYMTIPQLEETIYYIRMKLEENERAEVTRLIKVKNMGTTD"
     gene            62151..63506
                     /locus_tag="IV334_000235"
     CDS             62151..63506
                     /locus_tag="IV334_000235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk family potassium uptake protein"
                     /protein_id="HAP8942335.1"
                     /translation="MIKQSKQIKKRLPAVQLLALGFFLLILVGGGLLTLPFFSNSGHG
                     TNFIDALFTATSAVCVTGLTTLNTAEHWNSAGQFLIMLLIEIGGLGFMMVPILCFALI
                     KKKVSLSTRIILKEALNLEEVSGVMKLMLYILKLAFVIQLIGTVALSFVFVPEFGWLK
                     GIWYSLFHAISSFCNAGFDLLGDSLANHQQNVYLIMVVSALIISGGLGFIVWRDLLEY
                     HQKKKMTIHSKIALTVTGTLLLGGFVVFFFTERNGATLVEGNMFQRLANTFFMSVTPR
                     TAGYYSVDYLHMSHAGLILTMFLMYIGGTSGSTAGGLKTTTLGVLLIQMRSMLKGRTR
                     AEAFGRTIRQGAVLRALTLFFVTLTLCVVAIMILSVTETIPKTSGIEYIAFEVFSAFG
                     TVGLTMGLTPDLTAIGKLIIIALMYIGRVGVLTVVFSLMTKAQRTQSKYKYPEESVMI
                     G"
     gene            complement(63698..65092)
                     /locus_tag="IV334_000236"
     CDS             complement(63698..65092)
                     /locus_tag="IV334_000236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HAP8942336.1"
                     /translation="MPINSYDNYPLTWRPDKQKLKRPYYRSLIQQLEQDILKNKLQKN
                     TQLPSQRELADYLDLNFTTVGKAYKYGIEKGLLYTNIGSGTFISPNVFESITISTNEV
                     TEHIIDFGLVSSFEECNKFVVPFIQSTSNNPNLVGLLNYQDPKGTNFQLTTAANWLKT
                     QGVIPSLESLSIVSGVQNGLAITLSALFSPGDRIATDRYTYANFIELAQLYHLEIVPI
                     DFDEQGMRPDILIQECRKKKIHGVFLMPSCNNPIGFQISHKRREQLAEVIEKEGLWVI
                     EDDIHSFLTTYYQHTIIPPFQNLLPNQTIYLAGMTKFICSGLRVAYLVVPVHVQSKIE
                     QAIFNINVKTSGLDVEVITQILHSNEANRILETKLSLTKRANQLFDSLFKVEKPTNRV
                     PYYRILPVDPEIDPKIIENDFLANGIRLYHSRRFTTRAQPDAFVRISLSSNNLEILAS
                     GLSIVKEKIKKYEL"
     gene            65184..66155
                     /locus_tag="IV334_000237"
     CDS             65184..66155
                     /locus_tag="IV334_000237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine cyclodeaminase family protein"
                     /protein_id="HAP8942337.1"
                     /translation="MKIINFEEVVASFSFEEAIQVMKKCFFDYESGKISQNPRLVEIL
                     PDGKGKNMFAMMPAYLGENRYFGTKVITAFPDNHQYHLPSHLGEIFLFDAQNGLPKAL
                     INANAVTWIRTAAVSALATDYLANPHAETLALIGAGQQALSHLTAISTIRPITTVFVY
                     DILEERRNTFIAKAKKQYPKITFINAISVEDAVRPAEIICTLTSSKEAFLQKEWVRED
                     AHINAVGTFTPDTREISTSLMETSQIYVDDYSAALTESGDLLIAMAEGDLEESCIVGT
                     LGELVSEKKTFYKGESRPTIFDAVGLAIEDLCCAEYIYNKLEKGALK"
     gene            66152..66934
                     /locus_tag="IV334_000238"
     CDS             66152..66934
                     /locus_tag="IV334_000238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis protein"
                     /protein_id="HAP8942338.1"
                     /translation="MNLDYYVVDAFADEVFKGNPAAVYVLEEWLPEGTMQKIAIENNL
                     SETAFTVKKNQEFELRWFTPDREIDLCGHATLATAFVLFNYYKIPDETIKFSTQSGNL
                     FVTRIKDYYYMDFPSIMPKKVPILSEYEEAIGAKIKEAYLARDLFFVLEDEKTVAKLQ
                     PDFTAIKNFELGIGVIVTAESVQKDFVSRTFFPKLTINEDPVCGSAHSNLIPYWAERM
                     NKTKLSAYQMSSRGGDLECELKEDRVIIGGKAVLFSKGTSFI"
     gene            complement(67103..67474)
                     /locus_tag="IV334_000239"
     CDS             complement(67103..67474)
                     /locus_tag="IV334_000239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HAP8942339.1"
                     /translation="MKMAHTCVRVKDLEASIEFYQKAFGFEESRRRDFPENKFTLVYL
                     TLPGDDYELELTYNYDHGAYDLGDGYGHIAIAADDLEKLHEQHKAAGLEITDLKGLPG
                     TAPSYYFVIDPDGYKIEVIRG"
     gene            67608..68051
                     /locus_tag="IV334_000240"
     CDS             67608..68051
                     /locus_tag="IV334_000240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HAP8942340.1"
                     /translation="MDKPIFGEKKQFADYQTRYAAYIVIERDQQIAVIEAPNGAFFLP
                     GGEIEGEETKEEAIVRELIEEMGIVAEISSYLGEADEYFYSNYRATYYYNPGYFFVAD
                     SWKRIGEPTEKTNKIWWVSPQEALVKLKRGSHRWAVERWIEKNKN"
     gene            complement(68094..68903)
                     /gene="yidA"
                     /locus_tag="IV334_000241"
     CDS             complement(68094..68903)
                     /gene="yidA"
                     /locus_tag="IV334_000241"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase"
                     /protein_id="HAP8942341.1"
                     /translation="MSIKLVAIDIDGTLLNEKREVTKEVKEAIAAAVAKGVSIVLCTG
                     RPLPGVQEQLNELNLFQDNDYVITYNGALVQQTKSGKIIARHGLTHEDFLEIEVMARR
                     VGSHLHSIDDKAIYTPNRDISAYTIHEASLVKMPLKYRTPEEMTPDMNIVKMMMIDEP
                     EILDAAIARLPQTFRDKYTTVKSAPYYFEVLNKEASKGAAVANLAQHLGIDQDEIMAI
                     GDNENDLSMIEYAGLGVAMGNAVSLVKEAANVITSSNDEHGVAEAIKKYVL"
     gene            complement(68922..70289)
                     /locus_tag="IV334_000242"
     CDS             complement(68922..70289)
                     /locus_tag="IV334_000242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HAP8942342.1"
                     /translation="MTTPYKYQMLPMEKVFRDPVHNYIHVQHQVILDLINSKEVQRLR
                     RIKQLGTSSFTFHGAEHSRFTHSLGVYEISRRICDIFSRNFSKEKIGNGGWDDQERLV
                     TLCAALLHDVGHGPYSHTFEHIFHTNHEAITVEIITSPETEVYQILNRVEEGFPEKVA
                     SVIQKTYPNPQVVQMISSQIDADRMDYLLRDAYFTGTEYGTFDLTRILRVIRPYEGGI
                     AFSMSGMHAVEDYIVSRYQMYVQVYFHPVSRGMEVILEHLLHRAHELYQEDASSFELH
                     SQLLLPFLDGSFTLEEYLKLDDGVLGTYFTQWSESADPILNDLAKRFLNRKPLKSATF
                     SGNRDSKLVQELTLLVEKVGYNPVYYTAVNSSYDLPYDFYRPEQGRHRTQIEILRNDG
                     TLIELSQVSQLVAALAGQEQGDERFFFPKEMVDPSLRDHYDLFDETYQEFASHIRNGA
                     LIEIN"
     gene            complement(70273..70953)
                     /locus_tag="IV334_000243"
     CDS             complement(70273..70953)
                     /locus_tag="IV334_000243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014834505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase family protein"
                     /protein_id="HAP8942343.1"
                     /translation="MKDVRTPFLSDGIQTLKDAGYAPVIRNSGGLGIVSDEGILNISL
                     IFPQSDTKKITIDDAYEQMLLLTKYAFPKAPIQAFEITHSYCPGTYDLSIHGKKFAGI
                     AQRRIKNGISVMMYLSVNGNQEKRGQVMKRFYQSALKEDFGTNGYPAVDPATMATLEE
                     LLDQPLFVKEVKEAFAKAFEKLYSASDLQDSTDWLKKEIQLEQWNNQIQRMEQRNNIK
                     ELHYDNPL"
BASE COUNT        23460 a        12130 c        14803 g        20677 t
ORIGIN      
        1 agtagcagcc tagtcgtttc tatccttttt tgttatgcac tctttttgac atttactcca
       61 ccaataacga agggtacaat aaaaagtaat aacaaacaaa aggagtacag aaatgattga
      121 attcatcaat gtaagtaaaa gttacgggaa aaaacaagca ttaaaagact tgtctctctc
      181 gataagacaa ggggaaattt ttggattttt aggacataat ggtgccggaa aatcaactac
      241 gattaaaagt ttagttagta ttattgaacc atcatcggga acgattttag tagatggaat
      301 gaaattgacg gaaaatcgat tgagtatcaa acaaaaaatc ggttatgtgc ctgattcccc
      361 agatattttt ttacaattga cggctggaga atattgggac ttgatttcag ctgcctatga
      421 attagacaca caaaaaaaag aaaagcgttt agcagaattg acagcgcttt ttgatatgta
      481 tagtcatcaa aatgaaacaa tcgcaagttt ttcacatgga atgcgtcaaa aaacgatttt
      541 gatcggcacg ttgctgccag atccagatat ttgggtattg gatgaacctt tgcaaggact
      601 tgatcctcaa gcagcatttg atttgaaaga aatgatgaag gctcatgccg caaaaggaaa
      661 aacagtcatt ttttctacac atgtattgga taccgcacaa cagttatgcg ataaactggc
      721 tattttgaag aaaggtgaat tgatttatca gggggcagtt agtgaattat tgaatggctc
      781 acctgatgaa acgctagaaa agatttattt gaaaatggct ggaaggcaag cgacaggaga
      841 tggaccgtat gtataagcga caattattag aattaattcg agttaatctt ttatatgtta
      901 atccacagct cacgaataaa gcaagagaga aaggaaaaaa gggaaagcga ttaactatct
      961 atcttttgtc tcaaaatctt ttttcagggc tgattttttt actgatttac ggatttgcta
     1021 tgtttgctat gaactttgtt aaattgccag gtttctttac ttattacgtg gcactttttg
     1081 gtatgttagg tttaactcaa agtatatcag tcatttataa tatctttttt gaaggaaatg
     1141 acctgcaagt atttttgcct ctgccttttg gacaagaaca ggttttttca gcaaagattt
     1201 tggtagttgc tttgaccatc attccttttg tttttccgct tctagtcatg tttattttaa
     1261 ccggatggcg agctgatcta gggattttgc ttactatcat tatctcaatc cttttgtttt
     1321 ctctattttt gattattctt ttttgtctgg ctagtttgat tgtttttggt ttggctcgaa
     1381 cagcagtgtt taaaaaacat agaaaaacag tttctagtat cttgcttggc agctcgatgc
     1441 tgatcgcatt tgctggaatc atgtggatga atcatcaaac ggggactatt gaaaatgagt
     1501 taacagatcg gcatccgatt tcgatattat tgccattcta tgatgctgcc agtcagccac
     1561 tttctcaaca aggtttattg ggttggggaa tactagctat cacggctctg ggatttttcc
     1621 ttgctgtaaa acagctgata gtaccaaagc ttacagagca actgactgat tcaaacagtg
     1681 agttcaaaat aaaaagaacc cataaaaaga accaaaatgt gcatcagctt ttatttagtt
     1741 acaatgctca attagtaaaa gaacctaatt tgattatgca agttctttct tcttccttgt
     1801 taactccaat catttttatt cttgcttttg ccctaggagg cgaaataaaa ttaactgatt
     1861 tagataataa gttaatcggt gttgttttcc tggtagggat cgcattggct ttcttaacag
     1921 ttaatcagac ttcttttatc agcaatctta tttctttgga tcaagaaaat tttctgttta
     1981 tccgttcttt gccaatctct atgaaccaat atttaaaaga aaagttcaga ttcggatgga
     2041 tattgcagag ttttttaact ggtgggatcg cattattaag cggactgtct tttcagctgc
     2101 ctttgttttt tatccctagc ttgctatttg gttctttatt cggctgctac ttattgtcac
     2161 tacgttattt cgctagagat tatcggttgc tgctgctgaa ctggaccaat attaaccaat
     2221 tatttaacag aggttcagga agtttgggtt tagtcgcaac tatgatgggt tcattgatca
     2281 tcagtattat tcttatcgtc ttatacggaa tgttggcttt gttttatcca ttttggttag
     2341 tgaatattcc tgtgatcggt ttagttctaa tactttccgc tctatggatc gtttattatc
     2401 aacgaacgtt ttggaaaaaa tttgaataaa gaagaggggc cgtgacaaaa atcatgtcgc
     2461 agcccctttt ttacagatag agggtgttca aaagtcagtt tctcagtatt aagtcaattt
     2521 tatgtaaaaa tgtgagaagc tatttttaca aaactttttt taatactcat aattacttct
     2581 tattgctcct gcggttactt gtcgcaatgt ttgtgagata ttttgtcatc gctcaaggct
     2641 gaacgtctct ttcacaacct tttctttgtt acacttcgtt tatttaaaaa gccgtttttt
     2701 tcgtttcatc atgattgcgt cacgctttac tttgaaatta cggatgttat ggttaaagac
     2761 ttctgataat acaagaccta aagcgattgc tccagcaatc ataattgctt ggaccgcata
     2821 atgagccgct tctcgatagg attcagtaac gagatttcga accgcttgat aagctaaacc
     2881 accagggact agaggaacca tcccgggaat attgaaaatc gttacgggca ttttcaaacg
     2941 tttagagaaa agatcgctga tgaatgctac tcctaaagaa ccaagcaatg aaccaaaagc
     3001 agcagatccg cctatttgaa cacagatcca gtagatcatc cagccaaaag caccagaaag
     3061 accacaacaa atcaatgaac gcctaggtac atttgtaata acggcaaatg ccgcagtcgc
     3121 taaaaaacta aaagaaaact gaataaatat cgtccccata cttgtgttcc tttcctaata
     3181 aaaaagctgg aaaataaaag caatcgcaaa accgatcatc gtagcagtca tcatcgcttc
     3241 tgttccgcga gaaactcctg agacataatg tccagctagc aaatcacgga tagcgttcgt
     3301 aatttgaact ccaggaacta aaggcatcac acatccgata atgattagat cctgattgct
     3361 tcctaagcca aaacgtacgc tgtagatagc ggctatgcca atcaataaag aagataaaaa
     3421 ttccgataaa aatttgattc taaaaaattt caaactatat agataagctg cgtatcccaa
     3481 tcctccaatg actaaagtca caggtaaatc ggtcatcttt ccgccaaata acaccataat
     3541 tgttccactg atcactgccg cgctaacgat ttgcaaccag acaggaaaga aagaacggtc
     3601 tcggtcgatt gcttgcagtt cgcagtaaag ttcttctaat gttagctctg aagcaacata
     3661 tttacgtgaa agatcattta cttttactac tttttctaaa ttaatcgaac ggttggtaat
     3721 ttgttccatt cggatggtcg aggtacgatc aagaccaata aagagaccgg tttgagtgac
     3781 atagctgacc aaacggaaat ttccagaagc taaagcaatt cgactcatcg tatcttctac
     3841 ccgatacatc tccgcattgc tttccatcat gattttccca gcgagtaaac aagtgtccag
     3901 caacagatct aattcttttc ccgtcatagc atcttctcca ataattgatt gattagtttt
     3961 attttactac aaaacatacc agaagcgtaa agaataaaaa aacttgagga aggaataaaa
     4021 gaggcgtata ctgtttttgt attgcgggtg tagtttagtg gtaaaatccc agcttcccaa
     4081 gctgatgtcg cgggttcgat tcccgtcacc cgcttaaaat aggttgtgaa aaagctgctc
     4141 tgcaacaagt aataagaaca gccaagcaaa aacaacttct tatgttttta acttggatgg
     4201 acttattacc gcagatgcaa gcttttgcgc accgtttacc aagcggttgt gaaatcagtg
     4261 tattgtcttg agtatgaaga aaatttttga ataataataa ataccttaca gatagagtgg
     4321 tgactgatta gagacgagga atccgtctct tttttgtatt tttaagccat cttaaaacag
     4381 gttttatttt ttgctgtttc tatttctttc tagtatttat ttatagggga acatatgtta
     4441 agatttaggg agctgttagt aaaggagtaa agaaatgagc aaaaaaacaa ccaaggcgat
     4501 cgtggggatc gtcgctgctg tgattgcagg ggcattcggt gtttccacca cacaaaaaga
     4561 ttcagatatc tttcagcaga ttacaactat ttttgattcc acaccacaag cttctcaagt
     4621 agcggatgtg ccagaatatg atggaaaaca tcaagaaata gaaatcaatg ggaatcagcc
     4681 ggaatttaca aaagaggatc ttagcctttc caaaggaaca tgggaaagct attcaaatat
     4741 tgataaattg aacagagtag gacaagccaa tgctatgctt ggaaaagatt tgttccctac
     4801 tgaaaagaga gagcctttgt atattgaccc gacaggttgg aaacaaaaga aattaagtga
     4861 cgggcaatgg ttatataatc gcagccattt aattggtttc cagctgactg gtcaaaataa
     4921 caacattaaa aacttgatga cagggacacg ttcgttgaat gctccataca tgcttgctca
     4981 cgaaaatgat attgcggcat atatcaaaga aacaaatcat catgtacgct accgtgtcac
     5041 tcctcatttt gaggggaatg aactggtagc caggggagtg caattggaag cagaaagcat
     5101 tgaagacaaa aaaattgaat tcaatgtctt tatctacaat gttcaagatg gctacacgat
     5161 caattatgag acaggacaag caacaaaaac aaaaaattag tagcagctat tcgaattttt
     5221 tatgaatatt atgaaaaaat tgtgtaaaaa catacattca aagaaatata gcgtatactt
     5281 tatgaaaaga gtacttttga taaagctaca gaaaaaagga aaaaaacata caaatagtgt
     5341 ttcttaattt attcggaagt ttggaatatt tgtttgtagt tttccctttc ttcgagtaaa
     5401 ataaggttgt agaaggtaag ctggaattac cttgcgggaa agaggtgtag aatcatgggt
     5461 tttacagatg aaaccgtacg ttttggtttt gacgatagtc gcaaaaaaga agtgagcgaa
     5521 acattagcga ttgtttatcg ggctttggaa gaaaaaggct acaatccaat caatcagatt
     5581 gtcggctact tgctttctgg agacccggcc tacattcctc gttatcgtga tgctcgaaat
     5641 ttgattcgcc gtcacgaacg cgatgagatc atggaagtaa ttgtcaagga ctacctctca
     5701 aatcatggag tgaatttatg agagtaatgg gcttggatgt cggttctaag acagtaggtg
     5761 ttgctatcag tgaccccttt ggctggacag ctcaaggagt agagatcatt cggatcaatg
     5821 aagcagaaga agagtttggc tttgatcggt tgaaggaatt agtagcacaa tatgaagtag
     5881 accggtttgt agttggatta cctaaaaata tgaacaattc catcggacca agggcagaag
     5941 cttccatgtc atacggagaa aagatcaaag aactattctc cttgccagtt gattatcaag
     6001 atgaacgatt aaccactgtt caggcagaac gtatgctagt agagcaggca aatacttcaa
     6061 gagccaaacg aaaaaaagtc atcgataaat tagcagctgt gatgatctta caaaattatt
     6121 tagacggctc aggtaagcaa ttattttaat aataaaagaa aagaggtaat cttatgacag
     6181 aacataacca ttcccacgac catgaaggac acgaacacat cacattggtg gatgaccaag
     6241 gaaatgaaac attatatgaa atcctactaa cagttgacgg acaagaagaa tttggacgta
     6301 actatgttct tttatatcct gctggtgttt cagaagatga agatgtagaa ttacaagctt
     6361 atgcatacat cgaaaacgaa gatggaacag aaggcgagtt agaacaaatc gaaacagacg
     6421 cagaatggga catgattgaa gaagtattca atacattcat ggcggaagaa gaagaataga
     6481 ctataaagct aggagaaacc ttgatatgat agggtttctc ctttttttat tttttactgt
     6541 atgccatatt gtatgccatc tagtcaatat ttaagtgttg tactaatttt tcaacagtct
     6601 tttctttcgt ttcttttgcc aagtgaccgt aacaatcgat cgtctgacta atttttgtat
     6661 gccgtaatct agtttgaact tctaccatag tagctccagc gtccaagagc attgaagcat
     6721 gagtgtgtct tgctacatgc acaccgagaa atggaaccat agctttttca caaatagtaa
     6781 tcaatcttgc ataaatagcg tggtgtgtga ttagttcttt atagatacca caaaaaatca
     6841 tagtcggatc agttacacca agtgtcagca tatatttccg ctgattaatc tgccattttt
     6901 ttaatataga aattgttctt tcatctagtg gaagaacccc agcactacaa tcagttttag
     6961 gatcactgat tttatagcca tttttggatt ggcttagtgt tttattgatg gtgaccgtat
     7021 tttcttgaaa atcaatgtct gaccaattca atgcaagggc ttcactgatc ctaaccccgc
     7081 tataaaagag aaagcgtagt aacgttttat caaagtcgtt tcgataagtt tcaggtttgc
     7141 tgtttaggta atcaaacaag gtgatcagtt gttctttact gtaaactttg accttttctt
     7201 tatgcttagt ggtggttctt ttaggcatta atacgttgtc tagtggatta gtctcaataa
     7261 tcccaagatt gatagcgtag ctaaaaatac gcttagaagt tgaatgaagt accttgtatt
     7321 ggtcagtagt ttcagcccag ctatttacaa tttgttgggc tttttttacc gtgacttcat
     7381 tcacacgaag atttccatat tttggaagaa tccactttct aaacttgatt tcagttttca
     7441 tgaaggtggc ttctcggaca gtggttttat atgtttctag ccacaagtca taaacttctt
     7501 taaacgtttc gttgttcgct tttttaatac cgtttttatc gtagtccact tgcaagcgtg
     7561 acaaggcaag ctgggcttct ttttttgttt tgtaacctcg tctacgaacc ttatcaggtt
     7621 tgccagtcat tttgttcatt ccaagatagg cttcatagag ccaaaattta ccttttttag
     7681 tttcatattg cttgaatgtt gccataatag ttttccctcc atagtgtagc aagccgtggg
     7741 ggcggttgga ggaggcagtt tattattatt ttgaatagtg tttatttagc tcagtaacaa
     7801 gagctgtgaa ctcttttaga agatcatttt cataaaattc ttgatactct tttttctcat
     7861 ctgtattttt ttctgaatca ttaattaatg agtcatatcg taaaagcgta tttagaagag
     7921 ctgcgagata agcaattggg gtactcccgt caggaaattc aattcgagat cctgttttaa
     7981 gatattttat tgcgtttgtt aaataaatct tctgtgagac agtcaggtta tcttcttcga
     8041 tgttattgat agcattagaa agattagaat caattataga gttatttttt tcaatagttt
     8101 ttttcaattg cttttcaagc acatttcgct taatttcggg catcatatgc atatctctta
     8161 atgtataaga tccgtttaat agataggtag tggatacatc taataccttt gaaagttgtt
     8221 taagcctggt gttattggga agattatagc cattttccca attactaacg gcacctttac
     8281 tggccggtgg ggataattta cctccaagtt gttccattga gagtcccttt tcaattctag
     8341 ccagtctaat tctttgtcct gtctccactg cttcggggta tttttttcta gccacatttt
     8401 caccaccatt catatatttt ctctactata tcacgattta aatatgtgtg aaacataaaa
     8461 gttcaaaaaa gtatgttaaa attcttgcaa atgaaaaggt atgatggtat attgagttta
     8521 taagaggtat agaaatgtat tcaagagcct cttgcatttt tttaaaatct ttgtatagaa
     8581 atgtatgcta acgagggggg ataaatatgg aattaaaaat tgacaaactc acgcttgatg
     8641 atccagcttt taaaattatt gacgatcgag ttgttcaaca agtagagcaa actcaaggaa
     8701 cgttactttc attattagaa aaaaagtacc agtttccaaa atatatgaac aaggcacaag
     8761 ctgctaagta tatgaacgtt tcatacaata ctatgatgca gaaatatgtt cctaacggtt
     8821 taaagttgat tatagttgat ggagtcgtaa gaatttcaca agatgaatgt gatcgcttta
     8881 tggaagaata ccaaaaataa acgcaagccg tgggggcaaa gggattaata ggaggaaata
     8941 ataatggaat cgacacaaaa taaagcaatc gaaaaagtat tatcaacaat tcttacagaa
     9001 gacacagcgg cagaattagc aaacttggaa ggcaaagcgc tagaagaaac gtttgaatgt
     9061 ttgtacgaac aaatggacta tcaaaaattg ctaccacaag aaccaacggt aagcggagta
     9121 ttgcgtggct tgaacgattt agtacaagca gagtttaaag aacgactttc tattgaagag
     9181 tatcaagaaa ttttgtatca gcaagttgag ttgttagcga gcttgctagg gcttgaactg
     9241 gaggacgttg aatgaaaact gaaatttgga acggacacat tataagattt gtcgatatta
     9301 atgatgaatg gtgggcagtg gcgaaagatg ttgcggaagc gttaggtctt aaacaggtta
     9361 ctagagccat tcactctttg cctaaagatg gggttactac tagtaaggtc atcgattcat
     9421 tgggcagaac acaagatgta aacatcatca acgaaaaaaa atatttaccg catggcattc
     9481 aaaagccgta aaaaagaagc cgaggcattt caagactgga ttttcgacat catcaaagaa
     9541 ctacgccagt ccacaggact tgaaggcttt caagtattcc gcatgctaga caaagaacac
     9601 caaaaagaag caatgactaa attaagccac gctattactg aaccaaaacc agtagactac
     9661 attaaagcga atgtgatagc caataaagct gtatcaacta tttatggtca ttccaaaatg
     9721 gtaaaaaaga aagatatgac tcctgaaatg ttggttgatc gtgaacctat tttagatgag
     9781 acagtagaat taatgactgt taaagaaaaa tatgggttgc agttttcagt gagtgagaaa
     9841 atttataatc gttctgctga attgcagaca acttaggagc attaacatga tcgagaacaa
     9901 agaaaaagcc ttatccataa cagtcttggc ggacaggata aaggcaactt aattgggata
     9961 aaacccgata ttctttgttc cgattatacc atattaaaaa atcggaggga accattatga
    10021 aacgaaactt aattatagga agcattttaa tcggcggaat tttcggctta ttaccattaa
    10081 ttgcaaaaat agctatcttt gcaggattag cgttatatct cttcactgct tatgacgagt
    10141 acgactatca attaagaact ggagggcata agtagatgta caaaacaaaa ctattaaacc
    10201 aactcgatag ccttgaatta gaggaaatta atcaagggat cgctgaacta gaaaataata
    10261 tcgggaaaac ctactttgga aattcattca atgaaaaact aacagttttg tatgtactca
    10321 aaaaacatgc agaacacaaa ataatttgtc gagaaatcaa cgagctaaaa aatcagattt
    10381 taaccgcatg gttaaacatt actgatatgc aagaagcaag ggtgaaaaca tttaatacat
    10441 gggtaaagta tcagaatcaa ttaaaaggtg ctgaatttgt tcgggatggg ttgaaatacg
    10501 aacttgagca gttgaagctc atggaggtgt ctgaatgatt tataccagta ttttgaaaaa
    10561 ggaggagagg tttattggca aataattatc aagcagcaac ggaattacaa aagtcgggat
    10621 ggtcaattta tcccttatct cctggaacta aaattccaat aaaaggctct agtggttgga
    10681 atgatgcaac taacgattct aatcaaatta atgaatggtg gacggaagat gcaactagaa
    10741 atattgggtt aatgcttgaa gatcatcaaa tggtggtagt tgacctagat caacattctg
    10801 aaacacgaaa cggcgttgag aattataaaa aacttgcttc aatgtatgat ccatttcctt
    10861 ctacttatac ggaaatcaca ccaggtaaag gaatccattt cttctttaaa attcctgatg
    10921 gtgtaattat ccaacaagaa acaggtgcat ttgctgatat cttcgggcgt gatgataaag
    10981 gtaaaagttt aagcgggatt gatattatca ctaaaggagt tccaatagcg ccaactatga
    11041 ttatttcaag aagtgttggg agagcatatg aacctattga aaacgtatct ctaaataata
    11101 ttgcaccagc tcccgattgg ctagtgaacc tactgctaaa aaaaccgcaa gaaataaaga
    11161 ctcaataccg acccgtagca aaaacaggga cagcaaaaaa attagatatg attgttcaag
    11221 gcgctggcga aggtagtaga aacgatcatt taactaagtt atgcggttgg ttactatggc
    11281 acggtgtaga taatcaaact ttagcagaat tgatttatac agcaaatgcg tacaatgaac
    11341 caccgctaaa agataaagaa gtgaatcaaa ttattcgatc gatgattaaa aaagatttga
    11401 gggggaaagc tcatggctca taagcataaa ttagatagta ttttagactt tcccgaagcc
    11461 agcgagcgtg aagacaatat cattgaattg aaaacctgga tgtcacgatt aagatgcaat
    11521 aaagacgatc agattaaaag taacagtgtt gtgaatgcag aattgatttt aaccaatgat
    11581 agcaatttag ctggaacaat tgcttataac gaatttagcg gatacattca cctattaaaa
    11641 gattctcctt ggatcaatcg taccgctgga gaatgggaag acagttttga ggatgcctta
    11701 acggcatata ttgaagaaaa ctacaatgta gtttttgatg acaacaaaat acacaaagca
    11761 gtagtaaatg tggctagaaa gaatgttttc aacccagtaa aagaacgtat cgaaaaagtt
    11821 aaatgggatc aacagcctag actggaaaca ctgtttattg accttttagg ggtagacgac
    11881 aacctttaca cacgagaagt aacaaaacgt tggatagtcg gatcagtcgc acgtatctat
    11941 aaaccaggaa taaagtttga gatcgttcca gttttggatg gaccgcaagg gattggtaag
    12001 agtaccgttc cagcattact ttacacggat gattttttca ccgattcatt agattcgcta
    12061 ggtgaaaaga aagatgatta tatgcagctt caaggcaatg taattattga acttggagaa
    12121 ttatcttcaa tgaacaaaac taaaattgaa cagataaaaa acttcatcag tgccaagatc
    12181 gataaaatta gacctcctta tggtcggaat gtcatagctt gggcaaggca atgtgtattt
    12241 attggaacgt cgaatgatgg gcagtatttg aaagatgata caggaaaccg ccgtttttat
    12301 cctttgcctt gtaagaataa gccaaagatg aatccgttta aaacaaagga tgaatatttt
    12361 cttcaagtgt tagctgaagc aaaggtgctt tttgataaag gacaacgaat ctattttagt
    12421 cccgatgaag ataaagaagt cttagaaatt gcaaaagact atcaagagga tgccaagatg
    12481 gagaatccaa ttaaagaagc aatcactaaa tatttggaaa tggaaatccc gtataaatgg
    12541 gaagaagctc cagcgtgggc gagacgttct tattatcaga actatcctga cagtaaatgt
    12601 aatgagaaaa atttacagca tttctataat ggagctacta tgcaacaaga gttttattta
    12661 gttgatagcg tactcaccgc tgatattttg gaagctgttt ttgataaaca agcaaaggat
    12721 ttattgaatg gtcgatcaga tgcagaaact aaaaagattg ccttgattat tagcagtatt
    12781 cctggttggg aacggaagca actgccaaaa agaaataaac gacgaggatt ttacaataag
    12841 tcaaatgcct atgaaaataa aaatcgagct ggaaagtaca aaaagtaacg ggtagtaacg
    12901 ggtcagtaac acctagcccg ttactagttt acccttactg ccccaaggga tacagcaatt
    12961 tggtaacggg gtaacacctt atttccttaa aacttttgaa atagttagct aatatgctgg
    13021 ggaataatgt tactggattt tacccgttac ctgttaccaa aacgctagaa cccttgtccc
    13081 cgttgggatt gagtggtaac gggtacctgt tacgtaggtg ttaccacgtg ttacgaatca
    13141 aggaggaaag taaatgctca atattatttc aacaaacaaa gcaccgaact ttcaatatac
    13201 agatgaaatg gatcggtttc taatgaacac gctggcattt agtgtgggat tggtaacaga
    13261 agattattcc acatttgatc cagaagtatt aaagataatg gaagaagaac ctgattggct
    13321 gcaggagtca gtagcgtggt gtcaatcgct agtagtggga tcattggtag atagtggcaa
    13381 ctatgatgat acaggtgagc taatggatga atttaattgt ctgcttaacc tatatgatcg
    13441 agcgagacaa cgagagctta catcgaatga ggataatttg tttttaaaca tccatgataa
    13501 gttcttggct ttgctactaa cagatgatga attgataact aatttattgg aggtagaata
    13561 attatgcata tggaaagtat tagatacttc gatggtgaga aacacattga gtattcaaac
    13621 actcaagaag atgtagactt tattagtttt gaggaagata aaatcactaa agttaatttt
    13681 aaagacggaa cgtgcttgaa gatagtttcg ccttatattg aatacaaatc aaaatgggaa
    13741 caagatgatt ctgacgaacg agatcgcttt ggttggtaat ctatggcagt taaaaaacaa
    13801 tgtaatcatg ctggatgtaa aacattgatt gattataggc agaagtattg tgggaagcat
    13861 aaagcaaagc aaacggcaat taagcgtgaa gaaagaaagc aaagcgaagg taaatatttt
    13921 caattctatc aaagtagaac atggcgaaag gcttcatact tgtatcggtt aaatcatcca
    13981 gtatgtgagg attgcctaga agaaggcttg ataaggaaag ctgatgttgt agatcacaaa
    14041 attgaactaa aagatgattg gaatagacgg ttagatgaaa ctaattttcg ttctttatgc
    14101 catgcacacc ataattccaa gacagccaat gaaagacaaa gacgagaaaa gagtaccctt
    14161 tgagttaagg gggcatagtg tgaaacttac tgacaatcga tgcctactca ttttggtaca
    14221 aataaccgtt gtgaaaaggc ataagggtaa ttacaaataa cgattacact tgtaatcaca
    14281 agtgaaaaag tatataatga aagtaggaga tttattgaaa aatacaattg ttattactga
    14341 taaaacaggg aaacagcgca ccattgagtt acctcatttt ggtcaagtga ccattcaaat
    14401 gcagaacgga aaaatcattt atatagataa attagataaa gaaaaattct gatcgaaaaa
    14461 cgaaggaatg tgagcttaat tgcttacgtt cctttttctt ttgtctgaaa ggaggacaac
    14521 atgggacacc ctaaaatatt acacgataca aaaggcaata tatcaagtga agaaaaagca
    14581 atccgtgtgg acgctagaga agaattgttc aagcagcaac cattaataaa tatcacgccc
    14641 cctgactgga tggcagcgag cgctagaagt gaatggaatc gtatcgtacc aacattaaaa
    14701 aaagattatc cattgagtga agcagattat ggttcattgg tagcctattg tttagccttt
    14761 gctcgaatga aaacagccga agccgagata agaaaatcag gtacgtttat cacatgcgaa
    14821 aacggagtaa agaaagccaa tccagcggtt cgggttcaat cacaagctat gagtgatttg
    14881 aaaaaacaag ccacctcact aggtatgacc ttagaatcac gatcaaaact agctttgaac
    14941 aaggttaaaa atgatgaacc cgaagacccg ttcaaagagt tgatgaattc atgaatgatt
    15001 acattgagaa agtcctatca ggtaagctga ttgcacccaa gaaaattatc caagcgtgtg
    15061 agcgtcatat aagcgatttg gagcgttcta agtcagatag cttcccttat gtgttcgatg
    15121 aagaacaagc caccaaagcg attaaattca ttgagttgct accatctacg gacggtaaag
    15181 caatcaagat gttaggattt caaaaattca ttctaggcag tctttatggc tggcgtacta
    15241 aagaagggaa ttacaggcga ttcaatcgag cgtttaccag tatgagccgt aagaatggga
    15301 aaacgtatat cgcaagtggc atggctgcca atgcgttgat tatggaacaa gaaccagcag
    15361 aagcaaggca agtattgttt gtaagtaacg ctttgaaaca agctaaattg ggctatgata
    15421 tgctgtctaa ttcacttaga aacgtggtca agtctagtaa gtttttaaga ccacaactga
    15481 aaattatgaa ctctaagatt cagcacttgc cctctaattc gttcgctatg gcactggcta
    15541 gtgaaaccag cacgctagat gggtttgcac caacaaccgc aattcttgac gagtggcacg
    15601 aagcaaaaac tcgtaaaacg tacaacgtca ttaagtcagg aatgacccaa caaaagaacg
    15661 gcttattgtg tgttattagt accgctgggc ttgatttaaa cgttcctatg tacgaggaat
    15721 acttattgtt agatcgtgtg ctaaaaggcg aagaacaagc cgacaggtac tttatagcga
    15781 tatgggaatt ggacgatccc gaagaaattc acgatcaaga gaaatggatc aaagccaatc
    15841 cgatttttga aagtgaagaa atcaaaaaag taatgattcc aaccattcaa gatgatgtga
    15901 accttgcttt gaaacaaaat aaccttaatt ctgtattggt gaaaaacttc aatttatgga
    15961 gacaagcgag tgaggacagc tatatgattg ctgaagactg gcaggcaacc gaagtagaac
    16021 cacaagatat tacaggcaag cccgtttata tcggagtgga tttatctaaa acagatgatt
    16081 taactagcgt ttcatggatc gtaccgctag ataacggcga actttattgt gattcacata
    16141 gctttgtagc caccaaatat gggcttcaag acaaagaaaa gcgcgacggt ttgccttatc
    16201 gagaactaga aaaagctggt gagtgttcca ttactcaatt agaaagtgga atcgtggact
    16261 atgaccaagt atttcagttt attcaagatt tgattcaaga aaatgatttg gaatgtatgg
    16321 ggatttgtta tgacccgtat aacgctaatt cgcttatcag taaagctgaa aaagccaact
    16381 acccaatgtt agaagtaaga caaggaacga ttactctaaa cgttccgacc cgaactttta
    16441 gagaacaagt ttatgaaggc aacgttattc acaataaaaa tacgattctc acccatgcag
    16501 tgaacaacgc tattttaaaa acggataaca acggcattca gattaataaa tcaaagaaca
    16561 gtaacaaaat tgatccaata gctgcattaa tcaatgccta tgtgtttgca atggattact
    16621 tcaccacaac ggaaggagcg aaagcagaca atgaatttta tacaagtgaa gaattttctt
    16681 tctaattaca ttcataccgt tcttttactt cttggattgg tgtgtgtgtt ggttgctatc
    16741 accttactaa caaatgtcta ctatggcttg ttagcgctgg gcatagtgct tattggggta
    16801 gcggtcatgc taaatacaga acagaaggga ggttaaaaga tggcattttt taaagcgaga
    16861 caaaatacaa cgggagatcc tttcctacaa catgtggtat caatccaatc ggatgattac
    16921 accaccagct ttacaagcgt tcgtgcatta agaaatagtg atgtgtttgc agccgttcga
    16981 attattgcta gtgatattgc ttcaagtccg attcaattgg tcaaaaacaa tatgccacaa
    17041 gctgataatg aactggtgaa gttactaaac gagaaaccta attcagaaat ggatggttgg
    17101 catttaaagt ttgctttggc ggtcaatatg ttgctaaacg gtaatagctt tgcagagatt
    17161 aaacgcaacg gtgaaaaggt agaagaaatt cacttattac ctaactcaag tgtaacggtt
    17221 actcaattag ataatggtac gttgtcttat cagattggtg ataaaaaaag acgtgtgaag
    17281 tctagcgata ttttgcactt caaatatttc acccaagatg gtttaacagg attaccacca
    17341 ctttatgcgt tacgtgatga actgaaaatt caacaagctg gcaaccgcac attgcacaat
    17401 ttctttactc gtggtgtcag tgggtcaggt attttgaagg ttcataagtc agatttagac
    17461 ggatcagcaa agaacgcaat tcgtgaaaag tttgaagaag ccaacggttc aagtagtgga
    17521 gataatgctc ttagaacgat cattcttgat gaaacaatgg actacaaaac attagaaata
    17581 aacaccgatg ttttgaagct tatcaattcc aatgattgga acacaaaaca aattgccaag
    17641 gcgtttggtg taccgattga gcgtttaggc gttgaaaatg aacattctag caccgttcaa
    17701 agtaatctcc agtatatcca aagtacgctg attcattact ttaatgtgtt tgtgagtgaa
    17761 ttagatacga aacttgaaac caatatccgc tttaattccg atcagttact agagactgat
    17821 cctgaaacaa aagtaaaaaa tgtattggat caggtcaaag ggtcacttct cacgattaat
    17881 gaggggcggt cgaaaatggg gctacccccc atggatggtg gcgatcgttt actagcaagt
    17941 ttgaacttta cgtatttaga tacgttggag aaatatcaat taaaagaaca ggaaggagtt
    18001 acaccagttg aataatgaag aagaaaagga aaaacggctg acagaagaag ctgagctaac
    18061 agccgattct cccaaagtag agaaagaaaa tgaaaaacaa ccaacagacg gcaaaactat
    18121 ttcaggctat gcgttgaaat tcgggcaacc gtcaaaagat ttaggcggct ttgtagaagt
    18181 gattacaccc gaagcattaa aagaggtgga tttatcaaat gtgttcttat tatatggaca
    18241 cgattattca aagccactag ctagtgtcaa agcaggtacg ttgaaattga atgtcgatga
    18301 tacaggatta cattttgaag cagagctaac cgacacgacc tattcaaatg atgtgtatga
    18361 gaatatctca aaaggggttg ttgattctat gtcctttggg ttcgtgcttg gattagattc
    18421 attcgacaaa aaagaagatg gcacaattga acgatcaata gataaaatca aagcacttaa
    18481 tgaaattagt gtcgtgaccg ttcccgctta tgattcatca aatgtccaag taaataagcg
    18541 ttcttacgaa tcgtttatga gtaacaacca agcaaaacaa acaaacaata gcttagaatc
    18601 cacttctaaa gcacaaaagg agagtaataa catggaaaaa actttaatcg ataacgaaaa
    18661 aacagaattg cgtgggtatg aagaatatat ccgttcacaa ggcgaagtgc gtgatggagt
    18721 cactactgta aatgcagcgg cagttgttcc cgaagaagta atcggtgaag tctttgattt
    18781 gaaacgttca aattataact tagctcaata tgcaacagta aaaacagtat caaatggaca
    18841 aggtaaatat ccagtagcaa ctaaccaaca agcagtgtta gcaacaaaag ctgaacttgc
    18901 tgaaattggt gatattgacg ctgaaatgtt tacttcagtt gattataaag tagaaactcg
    18961 tgctggtaag attgccttat caaatgaggt tgtggaagat tcagcagtga atattgtaca
    19021 agaggtcaaa gatcagttag cgaaattggt agaaaacact gacaataagc atatcatgga
    19081 tttattaaaa acattcacta agaaaacggc tgctacgttg gacgatttga aacaactata
    19141 caatgtggca ttagacccag cattaaataa aatggtaatt ctaaaccaaa gcggatatta
    19201 ccacctagat acattgaaag attcagatgg acgttacatt ttacaacccg atgtgacagc
    19261 acctagtggt aaatcattat tcggtatgcc agtagtattg attgcagata cattgtttgc
    19321 caatcctaaa gctggaacat tccctatgat tatgggggat attgcacaat ctatctttgt
    19381 tgctcgtaga aatcaagtaa cgactcaatg ggaaaaattc gattactact cacaaggact
    19441 tgcagtgatc gttcgcaacg attataagaa aattgatcaa aatgcttcag tgtatattga
    19501 gtttacgcca gttgtaacac caaaagtata gaaaaatatt gggcggcggt ttatcccact
    19561 gccttttttt cattaggaga gataaaatgg taattttgga tagcataaaa aaaagtatgc
    19621 ggatcgatca cactattgat gatgacttta ttcaacaatt gattgataca gcaggcgaat
    19681 atataaaaag tgctattgat agtagcgcaa cggataaaga tatggataat tatcagcaat
    19741 ttgatttggc ggtgtcatta cttactcaac attggtattt gaaccgtcaa gaagccagca
    19801 gcgaacggat accagtaacg gtacaagcat tagtacaaca aatgagaggt gcttattatg
    19861 ccaatcatta agaatgtaaa tgaattgact gagagagtca agtttaaaaa gacaaaacgg
    19921 gtaaaagatg aagatgggca gatggtggat agtgaagaaa ctgtatttga atgttgggct
    19981 aatgtgcgtt cacaaatgct aaaagacgtg cttgctagtg taggtaccat tcttgaagga
    20041 acgttgacgt ttattattcg atacgatcaa gattatgaac taacaaacga tatgaaagta
    20101 gcttggaaaa acaagagtta taaaattatt tcaatcaatg aaggaacggc gtttaaagat
    20161 tatacaacga tcatagctaa acagatttct tagaatgatt acacttgtaa acgttgtggg
    20221 tatttagtat aattaaggta gtaaatgaag ggttagctgc ctgaattttg caagacttag
    20281 ctagtcgaaa tctattgttg gactgaaaat tgtaggtgtg gttgcaaaca tgctggacta
    20341 agaaagatga tagatgatga acgttcaatt ttaatacagg catgctagga ctgattggat
    20401 ggttttggca agctgattgt gtagaaatca aagaacacgt ctgttgtgga cgtgtttttt
    20461 ttgttataat tcaaaagagg tgatttaatg gacaaagcag agtttacaaa tttttattgg
    20521 aagtattatc tacatttaga aaatgagttt attaatacca cagattttgt gatgctcgat
    20581 gagaataatt tcaaaacatt ctctattgaa tatcaaaaac tactactagc gattggttcg
    20641 gagtgtgaga ttattttcaa agaactttgc ggatttgata gcagtagcaa taaaagcata
    20701 actgatttta aagctgttat taaatctagc gaattaatgt gtttggataa tggtgttagg
    20761 gttatgaatt ctttaacatt gacttcacta aaaccttttg gagatgggtg gcctgaagat
    20821 acaccagatt ggtggaaaat atataacaga gtaaaacatg gtcgttcttc aaattataag
    20881 gaagcaaatt tggaaaatgt gttgtatgct ttagcaagtc tatatttatt agaggaatat
    20941 ttgcataaga aagtaatact agaaggtgaa gtagacttta taagtccaga gtcaaatcta
    21001 tttacgttat cttgggaaag aaaacattcg tcgatgcaaa aagtgacgtt tgaaaaagtg
    21061 gattcaaatt atgtagctcc actcattgat tttaaagaac aagaagaaat ttaaaaaccc
    21121 ccacggctta ttgaagtttt tgtatgccat attgtatgcc atttggttta aatcgcttga
    21181 aaaatagtga tagataactc taattcattt agtcataaac gctgttttga taacctttat
    21241 agttctttga tatgggtttc tcattcatgg cggaagaaga agaatagaac aaaagaatga
    21301 attaataaga tataaaaaaa gggattgtga caaacgtctt gtcacaatcc ctttttccaa
    21361 ataaacgatg ttcaatcagc tgcccctcgg cattcgctcg aaaaacggcg aaatatgaag
    21421 aactattttg ccatttgttt ctctaataat caggtcaaaa cgttgacttc acagtctctt
    21481 ccttaaacgg tgttctcctg ccaactcctc ggtcacaagt cacaatattc aacaaacatg
    21541 agaagctgtt ttttgaaatt gttccttgtg actcggagct gaacggcagg ctcacgacct
    21601 ctttcttaaa cggtgttcaa aagcttgcat ctgcggtaat aagttcaacc aaacgaaaat
    21661 tatgaggagc tatttttgtt tggctgttct tattacttga tgcaggacag ctttttcaca
    21721 acctcttttc gtttagcact aaatttcctt aaaaagtaga aaagctttcg aagcctcgtt
    21781 tgtttctttt actgtataat aaaagaaaag agtaacaatc gctaaaagat ataaacaaga
    21841 gaaaggaaat acaagatgat tggtctgatt tattttgtgg tgatcgttct ggcgaacaca
    21901 gttggcgcta tttccgggat gggtggaggc gtgttgatca aaccgattct tgatcttatc
    21961 ggcgcccatt ctgttgcagg gatttctttt tactcaacag ttgcggtatt cacgatgtcc
    22021 attgtttcga ctgtgcgaca agtatcaagc gggaaatcac tgaattggca gattgtcggt
    22081 tgggtatcta gtggagctgt tgttggagga gtagtcggca acattgtatt cgaagttttt
    22141 ttacagttat ttgaaaacga gaagcatgtg cagctgatcc aaattttttt gacggtattg
    22201 accttggttt ttgcgttctt ctacaccaag catcatcagc caaaatttca tttgacgtca
    22261 tggacttggt accttatatg tggggggggt tctgggcttt ttagctagtt ttttaggtat
    22321 tggtggtgga ccagtcaatg tttctttgtt gatgctgatg tttgcattgc caataaaaga
    22381 agcaacacta tattctctat ctacgatttt cttttctcaa ctggcaaaat tggtaacgat
    22441 tgctttaaca agtagcttta tgcgctttga tttatctatg ttgttttatg ttatccccgc
    22501 tgctgttatt ggaggtttgt ggggagcaag atttagcagg gttttgtctc caaaaaaagt
    22561 gacctttatt tttcaagtaa ttgtcctcgt ggttcttttg gtcaattgtt acaatgcctt
    22621 tgtcatctta caaacattag gagaatctag actaacgcaa gtcaaaatca ctgaaacata
    22681 ttgttctaaa tgaaaaaaag agtatactat gataaaaaaa ttcattttaa aaaatcagaa
    22741 attggctggt gatttggatg ttacgggaaa tgcgtttaga agatgcagaa gcactacgtg
    22801 aaatcaatgc ggatcaatta gggtatgata taccattaac cgttacggtg agacaaatga
    22861 agaaactgct gaaggagcca gagaaaaatt ttttcttggt ttatgaagat cctacttcta
    22921 aaaaagcagc tggctatgta caagcggagt cctatgaatc gatttttagc gatccaatgt
    22981 tcaatattat ggcacttgct gtgtctaaat atgctgaaaa aagaggcatc ggaaaggctt
    23041 tgatgactgg cgtagaagaa gaagcgaaaa aacgaggaat aacgacaata aggcttaatt
    23101 ctgcagaata cagagtagaa gcacaccaat tttatgaaca catcggttat cattccgata
    23161 aaatgcaaaa aagatttttg aaaatcctgt aaatctttat caaaaaaata cccgatcacc
    23221 aagccgatac ttgatgatca ggtatttttg tttttttgac taagaaagta attgcatcaa
    23281 gaaaccagag atgatcaaaa cgatggcacc accaagtcga ttgcccattt gagcaaaagc
    23341 aataagttcc atccgattag ctgcagataa gacggctaca tttcctgttc cgcccatcga
    23401 gttgttgcat aagccagcag taatagttga ttcaattgga tataaaccaa acaaacgacc
    23461 gacaaatcct gaaacaactg aaatcgcgat aacacttgtc agacataaga caacaaattg
    23521 ccatgtcaaa gcagaagcta atacattcag attcagcaac gcaataccga taccggcaag
    23581 aacagctgct gtcaagtttt tgacaatcaa gttattgaac atgatagctg catcttcata
    23641 atactctggt aataggttgg cgattttaca gatgacaacg ataataatca taaatgcgta
    23701 agcatggact ttaggaacaa aatagttacc aatttgtcct aagatgaaga aagtcaatga
    23761 taccatcaaa ccaacaccta actgtggaat ggaaggtttc acagttcgag gttttccatc
    23821 gccaagatcg cctttttcca ttaatttacc attgccgttg aattttggca ttgattgtcc
    23881 agcacgagca actaatgctg caccgatgat agctaagaca tttgtcattg ctgtcgcagg
    23941 aatcaggcgg gaaatgatag aagaagcatc cgtacctaaa acatttgcat aaatcgttga
    24001 taaaggaacg gctccggctc caacaccacc agccatcgca ggtaatgaga tgtataaaac
    24061 ggaatttgca aaaccatttc cgataagcat gcccacgagt cctacggcaa aaaaggagac
    24121 gaccattgaa ataagtgcaa cgggaataaa acgaacagaa gctttcatca atagattacg
    24181 gttcatacca aggatgctac cagtaatcaa ggcagcaata taaaagtccc aaaagcccat
    24241 attgctgaca aagttttctg tggatttcac tacatattca ggtaagatcc caaatgtagc
    24301 aatcgcagca gaagcgaaga tcgcaaaaac ggaaccacca cccaaataac ttttgacgat
    24361 agggattttt gtcccgataa aatgaaataa attaccgaaa ataaccaaaa ctgcgattgg
    24421 tccaatcata ttcgtcggta atttccctaa agccattgca atgatcagga caaggaccat
    24481 gatcaaataa agtggcaaac ttacgccggt gatttttgtg gaccaaaaac cttctttttt
    24541 cttttcctca actgcagttg cagttgttgt tttttgtgcc atttttacag atcctttctt
    24601 attctgtatc atatgaaaaa agccaaagaa aagcgacaaa agagcaggag ttggctggcc
    24661 gctgttttgc cgcagtcgct tttttctttt tagacgtcat acaaagtttc tgtatgttct
    24721 tttactataa cgatcttaat cttctaaaga tttatattca ggaacccatt tagcagattc
    24781 aactgcttct ttgatatccg tgatttcttc acgattcaag ttttgtgcga caactgattt
    24841 tgcgacagtt tcagcaatcg tttgagaaaa ttcagtgatt tttgctactg gaggcaagat
    24901 agctgctcct ggttttgtta catcaacgat tccgcctaat gctcgactag cttgagaaat
    24961 gatttcagca tttactcgtg tagctgttga agcaatcaaa ccaagtccta aacctgggta
    25021 catcaaagca ttatttgctt gaccgatttg gtaagttaca ccattatatt caacatcagc
    25081 agcagggata cctgttccaa ctaatgcttt tccgtccgtc cattctatta aatcttttgc
    25141 ttttgcttct gccaattttg ttggattaga caaaggcatg atgatcggac gtggtgtatg
    25201 agcagccatt tctttcacga tttcttcagt gaatgctcca ggttgggtag aagtaccaat
    25261 catgattgtc ggatggatcg cttttacaac tgcttctaag tttgtcaatt catcagcatt
    25321 agtgaaatct gagcgtttgc gagcgaatgg gatttgacca ggagtcaatc cttctgtatc
    25381 ttcaaacaat aaaccttgtt tatctacttg atagaaatgt tggcgtgctt cttcttcagg
    25441 tacaccttga cggatcatct catctaaaag gatgtttgcg atacccacac cagcagtacc
    25501 agcaccaaat gtcaaaatta tttgatcttt caattgttca ccggatatat tcaatccgcc
    25561 taatacacct gctaggacaa caattcctgt accttggata tcgtcgttga aagtagtgat
    25621 ttgatcttcg tatttttcta agatcgctgc agcatttcct cgtccaaaat cttcccaatg
    25681 caataacaat tctgggaata attcggtagc tgattgaacg aattgatcga tgaattcata
    25741 gtaagtttcg ccttctacac gagcatgacg attgcctaag tataagggat tattcaataa
    25801 ttcttcatta ttagtaccag cgtcgatcga tacaggcaaa acttgtgctg gattaatacc
    25861 agcagcagca gtatagacca tcaatttgcc gatagcgatg tcaacaccgt tcacacccca
    25921 gtcgcccata cctaagattc cttctgcatc agtcacaaca atcaaacgaa tgtctcgtcc
    25981 atctgccgcg ttcttcaaac ttgctttgat gttttctggt tcgtcgattg aaagaaatgc
    26041 agcatcttgc ggtttcaaga aaatttcgtt gtattgttcg attgaatccg caacgacagg
    26101 atcataaacg acaggcatga attcaactaa atgttcgccc atcaatttat aaaacaatgt
    26161 ccgattcgta ttgaatagat tcattaagaa gatacgtttt tccaaatctg tttgtttgct
    26221 taagtattga ccataagctt gaacagattg ttgttccagt gtttggacag tacttggaag
    26281 cataccagtg attccatatt ttgcccgttc ttcttttgta aaagctgttc ctttatttag
    26341 aaaaggatta tttaataagt ttaaccctgt aagcatgaat gattcctcct aagttttaat
    26401 acaaacgaag tatagaagtc agatgatgat atagtcaaaa agagcaggta tgataaattt
    26461 tatttatatg taatatagcc aaaagaaatc aaaatagcag tatttgttct ttttatatcg
    26521 acaatccttg cttgaatact atgcgtatct cttatatgct agagaaagaa aagtagaaga
    26581 ataagggata gatggaatgg ataaatagga tttctgagga aaaaagcaca tgatagcgtt
    26641 gataggagtc acctatgtca ggtgataaaa atacataagt agaaaattaa gaggaggaag
    26701 agaaaatgaa agaagttcaa ttgattagaa aaagcgaact atcagaaggc ggttgcaatg
    26761 catgtggcgt agtagaagcg acaagctata cattaaaatt agattccaat caagcaataa
    26821 tctctgaact gacagtaggc ggtcttgtgg acagtctggc gcttgctgaa ggttttactg
    26881 gagaagatat ttatgagatg ttttcagaag tccgtcagtt aaaaaaagga gaaaaatgca
    26941 tagaggttca tcatgagagc ccgaatgtga ggttcaaacg tggtgataat gagatgatct
    27001 tcaagaatca tgtgtctgat cacactgaac tatatgaaat cgtgaatcaa atactgtctg
    27061 ggatttttga attaggacct tatgagttca aggaagaaaa cggtaatcca aaattaaatg
    27121 aagaatggca agaaacgatt gaaacacaaa gaaacaatcc acacttattt caatagtctt
    27181 ccacagtatt tcgtaaaaat gttgatgaaa aagaaacacc gtgaatcaga ggtcgtgaaa
    27241 aacctgccct gcatcaagta ataagaacag ccaaacaaaa atagctcctc ctatttttcg
    27301 tttggttggg cttattaccg cagatgcaag tttttgaaca ccgtttaagg aagagactgt
    27361 gaagtcaacg ttctgacctg agtattagag agacaaatgg caaaatagtt cctcatattt
    27421 tgccattttt tgagctaata ccgagggtca gctgattgaa caccgtttta tcgagaggtt
    27481 gtgaaaaagt cgcttagttt cgagtagtaa gaaaaatttt gtgaaaatgg ctttccacat
    27541 ttttgcataa atttgactta ataccgagaa aatggctttt gaacaccgtt tattcaaaaa
    27601 aagggacttt gacactactt ttgtcaaagt cccatcggtt ttttatctat ttttattaca
    27661 atcatttctt ccagagtagt gacatctgga atggctgaaa tgtaaacaga ttatttgcct
    27721 gtatcaatgt cgaagagtaa tataaaaagt gtaaacgcta tcatattgtt catcattcaa
    27781 attttataga gagaaggcat tgagatggaa aaaacatatc aagcaggtac aaatgaagga
    27841 atagtagatt ttatcaatat ggaagattta gaaatcgcgg cttcacaagt gattccagct
    27901 ggtggttatg gttacatcag cagtggtgct ggcgacttat ttacttatca agaaaatgaa
    27961 cgggcattca atcatcgttt gattattcct catgtattaa gagatgtaga attacccgat
    28021 acaactacac attttgacga agaaatgctg actgcaccaa tcatcatggc accagttgct
    28081 gctcatggat tagctcatgt gaaggcggag aaagcttctg caaaaggtgt agcagatttt
    28141 gggacgatct atacagcaag ttcttacgct tcttgtactt tagaagagat aagagaagca
    28201 ggcggggaga aagcaccgca atggtttcaa ttttatatga gcaaagataa tggaatcaat
    28261 ttggatattc ttgaagtggc caagcgtaat ggagcaaaag caatcgtatt gacagcggat
    28321 gcaacagttg gcggaaaccg tgaaacagac cgcagaaacg ggtttacctt cccgctgcca
    28381 atgccgatcg ttcaggctta tcaatcagga gtaggccaaa cgatggatgc tgtatataaa
    28441 tcttccaaac aaaaacttag tccaaaagat gtagaattta tcgctgctca ttctgatttg
    28501 ccagtctacg tgaaaggtgt tcaatcagaa gaagatgtct atcgatcatt agaatctggt
    28561 gctggtggaa tttgggtatc taaccatggt ggccgtcaat tagatggggg gcctgctgcg
    28621 tttgattcgt tgcaatatgt ggcagaagca gtagataaac gtgtaccaat cgtgtttgac
    28681 agcggtgtac gacgtggaca acatgttttc aaagcaatcg catcaggtgc cgatcttgtt
    28741 gctattggca gacccgttat ttatggattg tctttaggag gaagtacagg agtccatcaa
    28801 gtgtttgatt tctttaaaac agaattggaa atggtcatgc aattagctgg aacacaaaca
    28861 gtagaagata tcaaaaaaat aaaattgcgt gagaatcgtt ttatttaaga aataaatgac
    28921 gaaaaaaata ggagtgcggc agatgatgcc gtactcctat ttttatagtt caaggatgtc
    28981 actaatagaa taatttgaaa cgaaaaaata aaattgaaat attttttcat acttaataaa
    29041 aaaggaagaa tattcatgta aacgatttta ttgttttttt ctcttttata caaaaagggt
    29101 cataatttca agtaagcgga tgaattcatg aggatttttt gcatactgtc tctactatac
    29161 ttaggtcact aagaaaaagg agtgatttat tatgaaaaag aacatgattg ggacattatt
    29221 actttcaaca cttcttctag gcggcttggc aacaccagct tttgctgagg gtcaagcgac
    29281 ttcaaaagga gatattacct ttactgaacc aacaaatacc gttgagcctc ttaaccctac
    29341 tgatccttca aaaccagtag agccagcaga tccggaaaat ccagcaactg gccagacagg
    29401 ttctttaaca ttggatgtag ttcctgaatt gccatttggt acacacgaga ttgaaagcgg
    29461 aacaaaaacg tatcaagtag acgcttcaaa aaacgataca ccttatctcc aagtatcaga
    29521 tcgtcgaggt gtaggtgcag atggccaagc acaagggtgg aacgtgacag tttcggtttc
    29581 agattttgta aacggttcac aagttttgca aggcgctgaa ttagattttg gaacatccac
    29641 tgttaaaagt acgtcagata acgaatccac tggaccaact agtcaaactg taactgggtt
    29701 gagtaaagca agtgcagcga caccaatatt tacagcagca aaagatcaag gtctgggaac
    29761 atggctttct gtttatgatc cagcaaatat cacactgaaa gtgccgaaag cagcagctgg
    29821 aacattcacg gctgatttga cttggaacct cgtggctggc ccagtagcat agagtatgaa
    29881 ataaattcaa caaactgcgg tgaaaatact ataggtgaag gattttttac cgcagttgtt
    29941 gttgaaagga tgtggtggac aatgaatcaa agtaaacgac tgtttgtttc gtttttttct
    30001 attttaagtc tatttttcat ttttccgtca atcagccaag caaaggattc agcaggagac
    30061 tttggaatca aaccggtatt tccagagaac caaatagata aagcaatcgg ttattttgat
    30121 ttattagtgg caccggaaca aaatcagaca ctagaagtaa ttatcagcaa ttcttcagat
    30181 gaagaacgaa catttgaggt ctctgtcaat ccggcggtaa cgagtgatgg tggaacgatc
    30241 gattatagtc aaaaaaaccc aacgttagat gagacattgc cgtttgatgt ccgtgacgtt
    30301 ttgctcattg cgaaaaagga aatcaatgta tcagcacatg cagaaacaac tatacctata
    30361 gaagtgaaaa tcccagcaaa aagctttaag ggacgtgtat tagcgggtat tcatgtttca
    30421 cctaaagaag aagctgaaac agaaaatgct acagaaggag cacaaatcaa aaatcgtatt
    30481 gcttataatc tcgcagtcgt tttacaagaa agtcaagaga cgatagaacc ggatctgaaa
    30541 cttttatcag gagatttgga cgaggtcaac gcaaagccaa ctgttcagct tcgtttccaa
    30601 aatcctcagc ctaggattat cagcaatttg atatttacga gcaagatctt ttatgaaaat
    30661 cagctttata ttgaaaatac gtctaatgaa tttttagttg caccaaattc gaattttcac
    30721 ttgaatcttg atttagctgg tgataaagca aaagccggcg attaccgcgc agaaatcata
    30781 gcaaaatcag gcgacagtaa tgaatggcga tttacacaaa actttacgat caaaaaagaa
    30841 aaagcacaaa aagtaaatga aaattccgtt tttgcagtac aagaacaatc tttcccatgg
    30901 ctttatgcag gattggttgc tgcagttcta gtgttatggt tgattttgct gtttttgtgg
    30961 gtccacaaga aaaagaaaaa aggagaaaca acatgatgag gtgatttcgt tgaaaacatc
    31021 gatcttattg ggagggatta ttgcaagttt gctgttcttc agctggcaag agccagtcca
    31081 agcagatacc aaaagttcag gtacgattac attttatgat ctaagtccag ttccgcatcc
    31141 aagtggttca gtcaatggcg catcaaaaca gcagacacag ctaccgattt ataaagaatc
    31201 taatcaacat ggcgtattcc cggagacgaa tgaagaaaag atccgttttc tttatttgat
    31261 cattggtata gaattatggc ttcttgcgtg gttgttgtta tggagaagaa aggaaaagga
    31321 ggatgaacat gttgctacaa aataaatttt cattttttcg ctccttcttt tttacttcgc
    31381 tttttgtttt aagtatattt atcttttcac cttcggcaga agctgttgga gcaacgcctc
    31441 ctgccatttc ttatgatcca tacacgtatg aaaatatcgt acctccaaat ggtggacgta
    31501 gtgcagtcaa tggcgggaag cttgtccaac taactggggc taaccaattg cctaaattga
    31561 ctgctactta tttgaacgat ggttcaaatg atccttggaa aggtggcacg gtccgttttc
    31621 attgggaaag ccatgtgaaa ctaacaaatg tacttgattt ggtgaatatc actgcacaag
    31681 gatttgcatt tgtggcgatc atcaatttgc cggaacatgt atcaagcagt gatgtattaa
    31741 aagcaattga ctggaacaac gcctacttgt atattgccgg tgcccccatc aaggttacta
    31801 gcggtagttt attatcagca ggaagccata ccttgcgttt tgggatgggg tcgtgggata
    31861 caaatactct attggcagat attattgcta ttattacgcg aggcgaattc aacgtggacg
    31921 atatcccgct tcaatttgat gttacagcta atgtcgcgaa gatgacagaa aacggagata
    31981 aatatgatac gagtccaaac aaaattttga ccaagggtaa attacaacct tctatgtcta
    32041 aaaaggccgt tttttctgtt gatttttatg attcgaataa tattatccaa gatcgtatgt
    32101 tcaattcgat tttagctcca gctgcaggta gattttatcc taaattaact ggcggaaact
    32161 tgaatccagg agcatatgca ttttctgcaa gtgcagagat aaatacgtgg aatagttaca
    32221 tctcaacaag tgattcaaca ggaaaatata atacatttgc tgataccaca gaatacgtgg
    32281 atggaaataa ccagaacctt ccaggctcta ccaatagtcg gacattgacg atggggctga
    32341 ctgaaggacg ttttgatcag ttgccattta gtcgctttga tcgcgtagtc aactatttta
    32401 caggaaaaaa cgttacacaa ggagcaatac tgactcatac tccagcaagc acaagtggat
    32461 tgaatcagac agccaatatc gtgtacagtg ggaaagatgc gcaaggcgga ttgctttctc
    32521 ctgttgcttt aaaggtattg gagcagtaca agctagacgg aacggtttta ccaacaaact
    32581 taacagccga tagtcagtgg ggaaagctcc tttcttctgc atcgtaccaa gtcacggcca
    32641 attggtcagc aactgaacta aaaaagggtt cgatccatta caggctttat tcgaaaggaa
    32701 cccaaaaatt agcgacagga tggacagatc aacttttcca aacgatccaa tcaaataacg
    32761 ggggtgtaaa tacagctact tctacactac ctgcattggc ttcagggcaa tattattttg
    32821 attatcgctt ggtggacgat gtacttacta acatgtatcc gtcttttgcg tacaaatggc
    32881 agtctgaaca agctggaatc accactcttc ctcaaataac agtggctaat tttccatctg
    32941 tgagtgcaga tagtcagcta aaaaacctta gcagacaacc tgagacagga gatcccctgt
    33001 ctgctctatc aggagatacg attcaagaaa atgtgacatt cacgttaaca aaatctgggg
    33061 atgcgataac cgacaaaaaa atcacgatct cacttcctga aaatactact tatatcaatg
    33121 gttccttaaa attaaatggt acaactatcg cagataccgg cattcaacaa ggagtttctg
    33181 tcccagcaga tctattgagc aaaataggag ataccattca tcttacttac aactatcaat
    33241 taaatactgt agatacttct gtacaatccg tcagcatact gacaaaggca gctgtacttt
    33301 ccagcaatat tacattagca gatggtgcta agcttcctaa cccagttgtc caaacatctg
    33361 ctaaaacaat tcttgtcccc aaacaagaat taacattagt taatgtacca gatgatttta
    33421 catttggtaa cgatctcccc aaaccgttga agacaagtta ttatgaagca aaaggcgatt
    33481 tttcttttga tgtgcgggat actcggcttc caagcacgtc tccttggcaa ctgacaggga
    33541 cattgacttc tttatttaag aatgatcaag gtcaagaatt aagcggaaca aaattatact
    33601 tcaatcattc tggatcaaaa caattgattc agcaaggtca aaacacgttg atttatgaga
    33661 gtgatggaac agctaaagga gaggttctcg ttgactttcc cgataccgat ggtttactct
    33721 tagaggtaaa tagcagtacg aatgctcaac ctggcgcaac gtatcaaggc atggtcacat
    33781 gggaactgac agctgggcca accagctaag tgattagaat ttgtatggat ctgtgataac
    33841 tgtcacagat cctctttttc aaggatgaga aggaaacttc tgctgttaaa atcagaaatg
    33901 cttggaggag tggacttgga acttttcagt ttgcttgaaa aaaatgaaca acggcaaatc
    33961 aaacttttcc aacttttatt agaggaaaaa ggagagattt ccgttaataa cgtacttacc
    34021 cgcttatcag ttgatcgggc taccttgaaa gaagatctgt tttatttaca agctaacttg
    34081 gaaaatatca aacaattgga aattgttatc caagaaggga atattttatt aatcagacat
    34141 ggaaatatag cagcagtcga tgtttattat cattgctact tgcgctattc tacaaaatat
    34201 cagatacttc tttatctgtt ggaaaatggc tcatttgatc ggcagaaact tgtcatggaa
    34261 cttaatatca gttcggctac tttgtctcgc agagtgaagg aattaaatga ttctttaaat
    34321 gagtttcatc tgaaaataaa aagcgggcgg ctgaccggtc cagaaagtca aatttgttat
    34381 ttttatttcc aattattttg gtttggacgt ccttatcttg aaaacaaaaa agaatacagt
    34441 gatttatcag gaaacacttt tttagatctg ctgcaaaaag agtttagttt tactattaca
    34501 gaagaaggac gattgaaatt tgaaatatgg ctttccatta tgaagaagcg aatgcgctgt
    34561 acgaaaaaat ccaaaccaga ttatagcaat cttcctgttg gattgtttca aacgacacaa
    34621 tttttattgg acctccatac atttttgacc cgttacttct ttcaatatgc ctttccttgg
    34681 aatcaaaatg aaacgttggt tttttatctt ttcatgatca gtaactttac attagataca
    34741 cataactcgc atgtccagga gtttttgata agcgaaaaat acaaagacga cgtagtagct
    34801 caattgaatc gaacgtttaa acaatcgata gaagaatatt tctcttttta tgtttttcca
    34861 gaagagattt cgcaaaagat tgcgttgaca gtgatgcaaa atcacgctca ttttgcctat
    34921 tttagaggat ggatgaatat ttttggccaa gaaggattaa aacagcggtt aactgaagta
    34981 gaagataaac aattcctggt tttatgtgaa agattggtca gacaaacatt tgctattgtt
    35041 gattttactg aagtagacga ggaaaatgcc cgctttgaat tatttgctcg ctatgcatct
    35101 attttgcgga cgctgatcaa gcagttagaa ttacgtctgc aaattgctta tgatcttcct
    35161 tatgaaaggc taatggcgga tatcgtgaaa gaaatcatcc aggaacaatt gctgaatacg
    35221 atcaaatatg atttagtcga atacgagaaa gaaaagcagt atgatatcat tttgactagc
    35281 caattgaaag agtacccgtc acagaagtcc gctaaagttt ttgtattcac tagcaacgaa
    35341 tataattatg atttccccta tttgaaccaa tttttaaaag aatgttattt agaaaaattg
    35401 aatttaagga tgaataaaac atgaaaatcg tgatcattgg tgcttcccat gcaggcatca
    35461 cagctgccct aaatctaaga aaacttcaac cgaaagcaga agttttactg atcgatgaag
    35521 atcataaaga tggactaggc tatgtttcaa atggaatcaa cctttattta aaaggaaaga
    35581 tacgtagttt agctgaagtt gcccataaca tgcggactct tcaagaaagt ggggcaaaac
    35641 tgatcaccga atggagagta actgaacttg atccagacaa acatcagctc atcctcgcat
    35701 ccaaagaagg aaaaaacgag accataacct atgataaatt aatcgtagca acaggttcct
    35761 ctcctgtcac cttgtataaa caaattgaag ctgaaaatgt ctacacctat aaaaatctag
    35821 tgcaatctaa gcaagtactt gccgctttaa aagaagcaaa agaagtagtg atcttcggtg
    35881 ctggttatat tggattggaa ttagcagatg ctttgagaaa taaaggatac attattcatc
    35941 tagttgatta tatgccgaat gttttatcaa gatatttcga taaggatatg atcaacagtt
    36001 ttcaaaatca gctgcaaact aagcaaatca atttttatcc aaatgaattc ttgatcgatt
    36061 ggaaaaaatc agaagagaaa gtagtatccg tacaacttct atctcaagca ataaaggcag
    36121 atatggtcat tttttctgcc caaactcgtc ctaatacgac attgttaaaa gaaaaagtgg
    36181 ctttgtatga ggatgagact gtgatagtca atgaatatct gcagacaagt gatccggaca
    36241 tctatgcgat tggagatatc gtcccagtca gttttgacaa aaataaacgg catctctttt
    36301 tacctttagt tactcgagct gtacatatgg caagagcagt agcgctaact ttgagtggtc
    36361 agccaaccgc ttacgacctc agacaaaaga taacagcagc agtcattata gactactttt
    36421 taggaactgt agggctgaca gaagatgaag cgccattctt ggaacaaaat accggttcgt
    36481 gttcaggaga atttgatcta tttccacaat acgatgaaga aaacaaaaca gtaaatgcaa
    36541 agctgatcta ccatccggat acattagaaa taattggcgg acagctgata agccaagaat
    36601 tcttattatc agatttgaac ctgcttgcgg atatcgtgaa acacaaaact acgattccac
    36661 agctagcagt tgaggatttt ggatttctgt ctgaacacac ctctcgtttt cactacctca
    36721 atgagttagc ttttaaagta ctagctaaga gagcaaatag caatagagta gataaaagac
    36781 tgtgagaaat atcacggtct tttattgtgt ggggatataa taaatattaa tcaatcgaga
    36841 ataaaaaatt catataatga aatagcggaa gaattttttg agcataatat tcgccaaaaa
    36901 tttatcaagt gataaaattg ttgtataaaa tgattatttc aggaggagga gttaaatgtt
    36961 tttagcatta aatgaaatta tgcattcaaa gttacgttat gcgttagttg caggggtgat
    37021 gtttttgatc gcctacctgg tatttttttt gactgggtta gcttatggct tagctcaaga
    37081 taatcgaaca gcagtcgaca agtgggaggc agattcgatt gtgctttcga aagatgcaaa
    37141 ttcaaatcta gggatgtcca tgattactaa gaagatagca gaagaagtcg aaggtgggaa
    37201 agtggcgtat cttgcccaga cacctggagt cgtgactagt aaagacagta cagaagaagg
    37261 aaaaatcaat gttagcttct tcggtatcga taaaaatcaa ttcatcatgc ccaatcttgt
    37321 tgaaggaaaa gcttttgata acgatgatga ggcagtaggc gatattagtt tgaaagaaga
    37381 atatggtcta gccgtaggag atacagttaa gttatctggt agtgacaaga cgtttaaact
    37441 aactggcttt acggatcatg ccaaattcaa tgtctcacca gttctctata caaccatcaa
    37501 tgcttaccaa gagatccgct tcgaaaaaga agatacaagt gaaaatgcac ggattaacgc
    37561 aatagttgtc agaggtaaaa tcagtaattt acctgaagat ttagaacaga tcaaaatttc
    37621 taaattcatc aatgaattac ctggatataa tgcgcaagta ttgacttttg gatttatgat
    37681 cggatttttg atcgttattg cggctattgt gatcgggatt ttcatttatg tactgacgat
    37741 gcaaaagatt aatatctttg gtgtaatgaa agcacaaggg atcacaggag gatttattgc
    37801 gcgttctgta gtggcacaaa cattcatttt atcatttgtt ggtattcttc ttggtctatt
    37861 aggaacagtg ggaacatccc ttgttctacc tgatgcggtt ccattccaaa gcaattggct
    37921 gttttttggc gtcattagct tgttgatgct ggtagtagct gtgttaggcg cactattttc
    37981 tgttcggaca atcgtaaaaa tcgatccgtt gaaggcaatt ggataagaag gaggaagagc
    38041 atgaaagcaa tagaatttaa agaagtagaa aaaagtttcc aagatggcga ccaagtagtt
    38101 aaagcattga aagaaacaac catgagtatc gataaagggg aatttgttgc aatcatcggt
    38161 ccttctggtt caggtaaaag tacttttttg actttggcag gcggattgca gacgcctagc
    38221 agtggctctg tatttatcaa tgatcaagaa ttcagtaatc aaaaagaaag tgaacgcgtt
    38281 aaactacgtt ttaaagaaat tggctttatt ttgcaagctt ccaatctggt tccttttctg
    38341 acagtaaaaa agcagttatt attagtagat aagatcaaac atgaacatcg tcaaaaagaa
    38401 gcaaaagaat tatttgagca gcttggcgtg gataagttga tcaacaagta tccagaagaa
    38461 ctatctggtg gagaacggca aagggtcgca atcgctcgtg cattgtacaa tgatccgtcc
    38521 attattctag cagatgaacc aactgctagt ttggattcag aaaaggcacg agaagtagtc
    38581 gatatcttgg caaaagaatc aaaagacaaa aataaagcaa cgatcatggt tactcacgat
    38641 acacggctga ttgacaaatg cgatagaata tttatgatgg aagatggtgt attgaaagaa
    38701 aaagaataac caaaaagcag gtggaaaaat ggtccatcga tacaacaaag atacacagca
    38761 aaaaattttg aaagaagcga ctgacttgtt tatgtccaag ggatatctcg gcacttcaac
    38821 aagagagatc gcacaaaaag ttgggatcac ccaacccaat ctctatcatt atttcggtga
    38881 taaagaaaaa ctatatacgg cagttttaga aaaccactta aaagatgttg gaaaagcttt
    38941 gagagaaatc gttcaaacaa gcgaaacggg atttcaagta accttgacaa aaatggcgca
    39001 gtttttgatt gagacacatc tggtcgattt gtttctgatg cttcacgatc ttgaaagcaa
    39061 tctttcaaag gaaacaagag atcatctttt ctttctatgg aagaaaaatt atcgtgaacc
    39121 ttttgaagaa atcttttcag caaaccaatc tgtgctaaga aatgggattt caaaagaaat
    39181 cgcagcaagg cattttttct tagtgttagc accttacatc actcggtcga aaagacctgt
    39241 ggaagaaccg ctaaatgtgg cgcaattgat cgatttatat ttgaatggtg tattaggaga
    39301 agcgtaaagc tgttatatag ggagaaaaat agttaggtga agaagcaaga tatgacgaag
    39361 atcgttatat cttgtttttt ttatataggt agtttttcat aaatacaaat ttgttattct
    39421 ctcatgatta tcagggtata tttttattta ttatgtcggt gttccaggaa gtgttttaat
    39481 ttaataacga agaaattaat tgtttgtaga atgaaggaaa ctatttattg ggtagatgaa
    39541 tcaccacgaa tagtcaaaag gagatttcag gatatgccaa aaatgaaaaa aatcatttta
    39601 gcagtaatgt tattttctac ggttatatct tttacagccc cttttttgcg tacttaagct
    39661 gattttaagt tttgaccctt agtctggatc tacgaggtga gagtcaagaa tgaaaaaaat
    39721 aattggagct tttgtatttg caatcattat cattattatc tgttttcaaa tagatgtttt
    39781 tggtcaaaaa aagatagttg gacaaacggt cagcagtacg aacagtacgg ttacaaagac
    39841 aaaggaatca tttgatgata cagaacaaga gacattaatc aaaatcggaa aaaatgaccg
    39901 tacactatat acagatgact cgctaactac cccacttgca actataaacg gcggagagct
    39961 ggcggagttt ctttctgaaa caaaaaatgc ttatcagatc aagacaaatg atggctatac
    40021 tggatattta gccctcgaag atggaaccaa agtcacaaag aatatacaaa caaaaccgga
    40081 agagctatct gatgcagtga ttgtattaga tcctgggcat ggcggaaatg atactggggc
    40141 attaagcaat gatgaacaaa cagaagaaaa agatataaca ttaagtacag ccataaaagt
    40201 aaaaaaggcg ctagaggatg ctggagcaac ggtttatttg acccatacta cagatgaatt
    40261 agtacagctt ggagatatat gcgactacag tgaagaaaaa aaggcagatg tctttatcag
    40321 tttacacgca gactctacgg aatatgcgaa tgaagcaact ggaatcacga catattatta
    40381 ctatggacaa gaggaaacat tagcacagac aattgctgac tccttcactg atctgcctct
    40441 ggattcaaga ggaatctcca ctgggaacta tcaagttctt cgagaaaatc ttcaaccttc
    40501 tattttaatc gagatgggtt atatgaataa tgatagtgat ttaaaggaat tagtaacaga
    40561 cagttatcag cagcagattg ctgcaagttt gacacaagca ttgaccactt attttcagta
    40621 ataaataagc ccagccaaac aaaaatagct cctcatattt ttcgtttggc cgggcttatt
    40681 atcgcagatg caagcttttg aacaccgttt aaggaagagg ttgtgagcct gccgtttaac
    40741 tccgcgtcac aaggagcaat ttcaaaaaac agcttctcat gtttgatgac tattgtgact
    40801 tgtgaccgag gagttggcat gagaacaccg tttattcgga acaagggact gtgacatgac
    40861 ttttgtcaca gttccattat ttttttagca gcttcaaagc tttttctgta ttggcaaaat
    40921 gaagtttggc atatttttca agcataggca tgccgccttt ctccaatagt tttgccgcta
    40981 cttggtcaga tttcgttcca gctcctgatg gaagggtgat tccagcttca gctgatgctt
    41041 tgtgtaactc ttccaaaaag ctggcacgac tgatgataga agcagcagca acagctaaat
    41101 gatattgttc tcctttagta acgaagtaca gtttttcttg taattgattt ttttctaaac
    41161 gcgcatattt tttaaaattt gcttccggcg taaactgatc gatcaaaatc gcttctggtt
    41221 tttctggctc gatttttttt agcagcaaat ggatcgcttg attgtgcaaa gcgactttca
    41281 tccgaacggc attgtatttt ggttggatct cattgtattt ttctggagat aagacaagca
    41341 gtttataggg gatggttttt tttaagatgt cagcaagctg gatgatttgg ctatctttta
    41401 actcttttga atctctaaca ccaagtttac gtaattctgc aagctgatca cgtttcacat
    41461 aagcagcaca tactgttagc ggaccaaaat agctgccatt tccgacctca tcactaccta
    41521 aaacggataa ttgactgata ttttttggca aagaagaggc tgtttttgct ggcttggctt
    41581 gtttatcctg tgcctccccc catttatcag cttctattgc tgcattcgta ccttgaaaca
    41641 tcacttttcc ggatgtataa gcggtaatgg tcgtcccgtc tttttttgca gcaaagctag
    41701 tataaggaat ctgtttattt aacaaataag agcgatagta tgctttcatt tgactaagtt
    41761 ggtcgtttgt tgctttgata atgacgttgc tcataaaatt ctcctcgttt ttatacataa
    41821 aactataccg caactcacga cttttcgttt tctaagagat tgcgagacat agcatttttt
    41881 taaagtttat catataatag ttaagtatgt gaaaaatatt tgtggagaac ggtgaaagaa
    41941 ccaacttcat ggtacactaa ataaaacttt catcaaatag gaggaaatga ttcgtatggc
    42001 gcacgaaaaa acacgctaca aagcagtgat tgccaaccaa acatatacta ttatcggacg
    42061 ggaaaccaag catcatatgg atatcgtaac gaagctgatc aatgaacagt tagcagaatt
    42121 gaaacaatta tcaccacaaa tggataatga acaagcagca atcttgatgg ctgtcaatgc
    42181 gctttcggat caattgaaga aacaagaaag aatcttggag ctggaagaag aaacagcaga
    42241 attaaagaaa aaaatgatca aatttacaga gcttgaaaat cgtgtgaaac gcattgaagc
    42301 aatcgagaac gaagctcggg aagtattgaa agaaaatggt caagcagatc ataccattaa
    42361 aaatcatgtg gaagcacaac agattttaaa cgagaaacga aaaggacaaa ttaaacaaaa
    42421 atcttctaat taagatcctt ttatcaaaat aagaaaaatt taaaaaagtt agaagagaaa
    42481 ggaacgtagc atgctgagtt tgctgatttt atttttattg ctaattgcgt ttttttctgg
    42541 agcacgtcgc gggtttgctt tgcaaatcat attcgcgatt ggatatgtat tgtcatttat
    42601 tgctgcccag catttttaca aaccattagc aagtcatttg gaattataca ttccataccc
    42661 agctgtcacc ccaacatcaa agttagcatt ttttgatcag gtatttgctt ttcacctaga
    42721 tgaagctttt tatgcaggaa ctgcttttct gattattttg ctaattggct ggctgcttac
    42781 ccgatttgtg ggggtttttg tccatggatt gacgtatgtg ccgatcttga gacaggtaga
    42841 ctggattgca ggaggcatcc tgtcactaat catggcttac gttactatat ttttgatttt
    42901 gcagttactt gcctttgttc cattagtgtg gtccaaaatc aattcggcga gcatagtttc
    42961 gctcgtttta tcgttgagag aacgccattt cttaccaaca agatgtatga gctttgggta
    43021 acgaatattt tgaattaagg aaaatagtgc tggaaaatat ttcgtttttt gtacagagta
    43081 gtttatttaa gaaggctgcg tagcgtatgt ccggcacagc tcttttttca tagtgaaaga
    43141 taaggtgata aaatgaataa ccgaatcctt gaaacattgg aatttgaaaa agtcaaacag
    43201 atggtcagac aatttgtagt gactgcacaa ggaaaagaag aactggcaga attggtacca
    43261 gtgagtgaga aacaaacgat cacaaactgg cttcaagaaa ctgaggatgg attgaaagtg
    43321 caacgtctgc gaggcgggat ccctatacct aaacttgaaa atatccgtcc ccatatgaaa
    43381 cggatcgaga tcggagcaga cttgaatgga gtggagcttg cacaagtttc cagagtattg
    43441 tctacgacaa gcgaattgaa tcgtttcatt gatgatcttt ctgacagtga aatcgaattt
    43501 gcacgtttgt atatgtgggc agatcagtta gtcacgatcc ctgttttgag ccgccgttta
    43561 aaggaagcaa tcgatgaaga cggacgcgtg actgatgacg cttctcctga gttaaagagc
    43621 ataagacaga acattcggcg aagtgaacaa gctgttcgtg aacagttaga tgggatcgtc
    43681 cgtgggaaaa atgcaaagta tttaagtgat gcgatcatca ccatgcgaaa tgaccgctac
    43741 gttatcccag tcaaacaaga ataccgcggt gtatttggcg gtgtagtcca cgatcaaagt
    43801 gcttcaggac agactttatt tattgaacca aaacaagtgg tggatttgaa taatcgtttg
    43861 cgtcaatacc aaatcgctga acgaaatgag attcaacgta ttttaagtga actttctgct
    43921 gaacttgttc cccatcgtca agaaatcatc cacaatgctt atgtgattgg taaaatggat
    43981 ctgatgaatg caaaagcacg atttggtaaa gaagtaaaag ccatcgtgcc gggaatcagt
    44041 gaagacaatc atgtcgtctt gaaacaagca agacatccac tgatcgatca ggaaaaagta
    44101 gtttctaatg acatcacaat cggaaaagac tatcaagcca tcgtcattac aggacctaac
    44161 acaggtggga aaacgatcac gttgaagact ttaggtctgt tacaactgat ggggcaagca
    44221 ggattaccga ttcctgcagg tgaagaaagt caaatcggta tttttgagga agtatttgcg
    44281 gatataggag acgaacagtc gattgagcaa agtctatcga ccttttcttc tcatatgacg
    44341 aacactgtag atattttatc aaaagtcaat gaaaaaagtc tggtattatt tgacgagctc
    44401 ggtgcaggaa cagatccgca agaaggtgct gctctagcaa tcgcaatctt agatgatctt
    44461 ggtaaaaagt ctgcttatgt gatggcaaca acgcattatc ctgaattgaa agtttatgga
    44521 tacaatcggg caaatacgat taatgccagc atggagttcg atgtggatac actaagtcca
    44581 acgtatcgtt tgctgattgg cgttcctgga cgaagcaatg cgtttgaaat ctctagccga
    44641 ttaggtcttg atacagaagt tattgatgaa gcgaagcagt tgatgaatga tgagagccaa
    44701 gatttaaacg aaatgatcac agatttagag aatcgccgga aaatggcaga gaccgaatat
    44761 ctggaaatgc gtcattttgt ttctgaggca caagaacttc acgatgattt gaaagaagct
    44821 tacagctatt tctttgaaga acgtgaaaaa gaaatggaaa aagcaaagaa aaaagcaaac
    44881 gaagtcgtgt cagaggcgga agaaaaagca gaaaaaatca ttgctgatat tcgtaaaatg
    44941 caacagcaga ttgggcaagg aaatgtgaaa gagcatcagt tgatcgatgc aaagacccaa
    45001 ttagcaaatc tacatcaaga agaaactttg aagaaaaata aagtattgaa gaaggcgaaa
    45061 gaacaaaaaa cgttgaaacc gggagacgaa gtgttggtta cgacttacgg ccaaagaggg
    45121 acgcttttac gtaaaaacgg gaatcagtgg caagtagaaa tcggtatatt aaaaatgaat
    45181 gtgtccgaag acgagctgac gcctgttgct cctcaaaaag agccgacaca acgagttatc
    45241 catgctgtac gatctgaatc tagtagtcac gtgccgaatc aattggacct tcgaggaaaa
    45301 cgatacgaag aagcgctaag tgaggtagat caatatttgg attctgccat tctagctggt
    45361 tatcctcaag tgactattgt ccacggaaaa ggaacaggcg cacttcgcaa gggaattact
    45421 gattatctta aaaaccatcg gagcgtcaaa agctttgagt ttgcaccggc aaaccaaggc
    45481 ggaaatggtg cgacaattgt taaatttaag taaagcttac taaaggtaag cagatggttc
    45541 gctatccaag tatagaaatg ctatactaat catagatttt aaaaatggag gtctttgata
    45601 tgtcacaagt aattacagat aagacattta atgaagaaac agataaagga cttgtattga
    45661 ttgatttttg ggcaacttgg tgtggtccat gtcgtatgca agcaccgatt ttggatcagt
    45721 tggaacaaga atatgatgaa gaagaattca gaattgctaa aatggatgtg gatgaaaatc
    45781 cagaaactcc tcaacaattc ggtatcatga gcattccaac attaatgttg aaaaaagatg
    45841 gtcaagtagt agaaaaagct gttggtgttc attcaaaaga acaactacgt caaatgatcg
    45901 atcaatattt ataaaaactt acagcaaaaa taagctgagg gtgggactga atccagttcc
    45961 gtcctcggtt tttttgcggt tgagttaagg ttattcaaat taatattttt tgagtataat
    46021 tacgttgtca ggctggattg tttttttgac agacatgcta taataacttt aattgaatat
    46081 gaaggggagt accagatgaa cgaacgaatc aaaaacaaat tagctttact tcctgatcag
    46141 cccggctgtt acttgatgaa agataaaaat ggaacgatca tttatgtcgg caaagccaaa
    46201 atattaaaaa atcgtgtccg ctcatatttt actggcagcc ataatacgaa aacggaacgg
    46261 cttgttagtg aaatcgaaga ctttgaatac atcgttacag aatcgaatat cgaagcattg
    46321 ctcttagaga tcaatctgat aaagaaaaat gatcctaaat acaacattat gttaaaggat
    46381 gataaaacct atcctttcct taagataacg aatgaaaaat atccccgttt ggtgattact
    46441 cgtaaagtac taaaagacaa agcactgtat tttggaccat atcccgatgt cggtgcggcg
    46501 aatgagacaa agaagatcct cgatcgatta tttcctctga gaaaatgcaa acctagtcag
    46561 acgaaggagc cttgtcttta ctatcatttg ggacaatgtc tgtgtcctta ttatttcgac
    46621 gtagatccga aagtctatac gaaaatcgtt gatgaagtga aaaagttttt aaacggagga
    46681 cacgaaaaaa tcgagtcaga gatccatcag aaaatggaaa aagctgctga aaacatggag
    46741 tttgaaaaag ctgcagaata ccgagaccag atccgtgcca ttgagacaat catgactcgc
    46801 caaaaaatga caaatactga tttactggat cgagatgtat ttggttatgc gattgacaaa
    46861 ggatggatgt gtgtccaagt gtttttcgta agacaaggaa agctaattga acgagatgtt
    46921 tcaatgtttc ctttctataa cgaagcagaa gaagatttct tgacttacct cggtcagttt
    46981 tatcaggaaa atgagcattt cattcctaaa gaagtcttga ttcctgatac gatcgacaag
    47041 cccagtgtag aagctttgtt agctacgaaa gtgctgcaac caaaacgagg agaaaagaaa
    47101 aaattagttg aattggcgaa taagaatgcc tcagttgctt tgaatgaacg gtttgatctg
    47161 attgccagaa aacaggaacg gacgatcgga gcagttgaac gtttaggaaa cgcgatgaat
    47221 atcccgaccc caatacgtat cgagtctttt gataactcta atatcatggg gacgaatcct
    47281 gtggctgcga tggtcgtatt cattgatgga aaaccggcaa gaaaagaata tcggaaatat
    47341 aaaataaaaa cagtcaacgg accggatgat tatgcatcaa tgagagaagt catctatcgc
    47401 agatattcac gagtgattcg cgaagggttg cctttacctg atttgatctt gatcgatgga
    47461 ggaaaaggac aagtagatgt ggcaaaagat gtcttagaaa atcaattagg aatcgatatt
    47521 cctattgctg gcatggcaaa aaacgataaa cataaaacaa atgagttgct attcggtata
    47581 gatttggctg tagtaccgct tgagcgaaat tcaccagagt ttttcttact ccagcgaatc
    47641 caagatgaag tccatcgatt cgcaatcact ttccatcggc agctacgcag taaaaacagt
    47701 tttgcttccc gattagatga aattgaaggg ttaggaccaa aacgaaaaaa aatgctgctt
    47761 aaagagttca aatcgttgaa aaatatcaca gcagccacga cagaagaatt acaagaaatc
    47821 ggactgccga aaaatgtcgc acaaaacgta tttgataaat tacatcagga atgaggaaag
    47881 ggcactttac aaagaaagta aagtgtccta tcattttatg tacaacaaaa agagcgttta
    47941 cgttttgttg gtaaataata aatcgcttac gtctggtgtc taaggatata tgtgatttct
    48001 tgaagaagga gtgaagaaat gttagaagtc aaggatcttg ttaaaacttt tgggaactac
    48061 acagctgtcg atcatgtctc tttccaaatc cccgatggaa aaatcatggg gctaattggc
    48121 caaaacgggg ctgggaagac aacgactttt cgtttgatct tggatttttt gacacaagac
    48181 caaggagaag tgttatggaa tggccatcat cttggggaga aagattatga tgtcatcggt
    48241 tatcttccgg aggaacgggg actttatccg aaagtaacga ttcaagacca actgctttat
    48301 tttgcagaac ttagagggaa atcaagaaaa gagattgaac caaaaatcga tttttggatg
    48361 gaaaaatttc aggtaaaagg gaaaaagacg gataaagtaa aatctttgtc taaaggaaat
    48421 cagcaaaaag tccagctgat cgccacgtta atccatgaac ctaaattgat cattttggat
    48481 gagccattca gcgggttaga tccggtaaat gctgaattgc taaaagatgg aatattggaa
    48541 ttgaaaaaac atggttcatg tgtcattttt tctagccaca atatggataa tgtagaaaaa
    48601 atctgcgatc atttgatcat gttgcgaaac ggaaaaatgg ttttgaatgg aaaagtacat
    48661 gagattcgtg aatcatttgg ccgtacgaag ttattcttgg aatctggttt aagccaacag
    48721 gaagtagaag aaatcgaagg cgtgaaaaaa gtgatccttc atgaagacaa tacgttagag
    48781 attaccttag aagatcctga agctgggaaa gaaatcttta ctcgtgcgac tcagttcggc
    48841 tacattccta tgtttaacca gcaaccgcca actttggaag agattttcaa attgaaagca
    48901 ggtgaaatcg atgcgtaagt tttggattat taccaaagat gtctatttaa aaaatgttaa
    48961 gtcgatttct ttcttgatca tgatattagt cccttttatc atgctgggta tcatttatgt
    49021 agcaggaaac tttgctcaac aaaatagtga tgtcaataaa atcggcgtat tggctgataa
    49081 tgagcaaatc agtcagcaaa tgagccaagt caaaacagat gattttgagt tcaaagcagt
    49141 tgattctgaa gaaaaagcac aaaaagagct ttcggaagaa aaaatcgatg cgtatttagt
    49201 agtctcaact gagaatgata agataaatgg gatgctttat agcgaaaatt cattaggaca
    49261 aagtacacaa ctactgatac aacaacaact gactggcttg caatcgatgc ttcgagcaag
    49321 tcaattaggc atttcaccag aagaagtagc tagcttgagc caacaagcaa gtttgactcg
    49381 acaaaaagtc aattttgatt caaaaggaaa aatgacagtt ggagaagata actcgacagt
    49441 ccaatatgtc gtaagttatg tagcaacgat tgtcttgttc attattattt tgacgtatgc
    49501 gcagatcatt gcacaagaga tcgcttctga aaaaggcacg cggatcatgg aagtgatcct
    49561 gtccagtacg actgcacaaa agcattttta cggaaaacta acaggtgttt tacttgttgc
    49621 attaacgcaa atggcattat acggtattat ttttggtata ggatacagtc aattcaaaga
    49681 tatggaaatg gtcaaaaata tattagacgg tatctcattg gataacttga ttggaccatt
    49741 cttgtggtat tctcttctat tcatgatctt tggcattttg attttttctg ttttagcagc
    49801 tttatgtggc tctttggtca acaaagcaga agatacagca aaagcaattt tgccagtgac
    49861 ttatctatct ctaggcggat atatgcttgg attgatacta ggagcttcag atccaaacaa
    49921 tatcgttatt cggatcactt cgtatattcc attcttatct tcatatatta tgccggttcg
    49981 cttagcaaat gagacagtcg gtatgggtgg agcattgatt tccttgttgg tacttgtagc
    50041 cgtaacattc ttattgatga tcttgtctgc aaacatgtac aaatcgaatg tactcgtgta
    50101 tagcgaaggc ggattatgga gttcattgaa acaatcgatt tcaattatga gaaatgaacg
    50161 aaaaaaagca taaaatatag aaaaatatag aaggaagctg gacatttatt gttcccagct
    50221 tcttttttgt atagacctat ttctattttt ttattatatt ggatatatat attcattgaa
    50281 aatagagcat aacaacttta taatgttgag tataccaatt tttggaggcg ttttaatgga
    50341 ccgctattta gatttacttt cagagaagtt ccctacaacg gaagatgtca tcacggaaat
    50401 catcaatctc gaagcaattt tgcagcttcc aaaaggaaca gagttatttt tgagcgatat
    50461 acatggcgaa tttcctgctt ttgatcatat tctgcgtatc ggttcaggaa atcttaaaga
    50521 aaagatcaaa gaccttttct ctgaacaaat gacggaaacg gagatcagcc aattcactat
    50581 ttttacggct tatccagaat atgtgttgac gactgattgg tacaaaaaac aagataaatc
    50641 ccagctgatc catcgtttga tcgatctttt acggtttacg acagtcaaat acactcgatc
    50701 aaaagttcga aaaggattgc cgaaagaata tagctatatc attgaggaac tcttgtatat
    50761 cgatgatcgt atcaacggga aaaaagacta tgtaaaaaaa atcattgaac aacttgtctt
    50821 gatgaaggaa gaagaaaaat ttttaacaaa acttgcgcag acgatccaaa aatctgtcat
    50881 cgatcatttg catatcgtgg gtgatatttt tgatcgagga actcaagcag ccaaagtaat
    50941 ggatcgaatc atggattttt cctctgtaga tatccagtgg gggaatcatg atattttgtg
    51001 gattggtgcg tattgcggct ctgaggcatg tttattgacg cttttacgga ttgccgcacg
    51061 ctacaactat ttatttgaat tagagaaaga atatggatta aatttacgtc cattattttc
    51121 ttttgcccat caaacctatc agaagaatcc agtatttacc cctaaaaaca ggaaagatct
    51181 atctgaaaga gagcaagaag aattagaaaa gatccatcaa gcactttcta tcctccaatt
    51241 caaatcagaa catcagttgt tgaatcgacg acctgaattt aaaatggacg atcgtaaatt
    51301 gttggaaaaa atcaactatg aggaatcaac gatcgtttta gaaggtcagc tctatgagtt
    51361 gaaagggact tgttttcaaa cgatccagcc agatgatcct aaaaaattct tggaagagga
    51421 acaaaaagtg atcgatagtt tgatgtattc attccagcac tcgttaagaa tgcaaaaaca
    51481 catgacgttt ttgatcgaaa aagggggaat gtatctcacg aacaatcagc atgtactgtt
    51541 ccatggctgt atacctgtag atgaaaaggg gcagctctta gcatttgaat tagaccaatc
    51601 ttatcgcgga aaagaactgc tgggattttt tgaaaaacag ataactgaaa gcgcaaaaga
    51661 tttgactgca aatgaagatt tggcgaccga tttgatttgg tatgcgtgga gcgggccatt
    51721 ttcgccttta ttcggcaaga gtaaaatggc aacttttgag cgctattttc tcacagagcc
    51781 tcatacgcac gtcgaaaaaa gcaatcccta ttatcacttg cgagatgaag agtggttcag
    51841 tgaaaaaatt ttggcagaat tcgatgcaaa agaagaaggt tcagcaatca tcaatggaca
    51901 cacgccagta aaagtaaaaa aaggagagtc accgatcaaa gcaaacggaa aagcatttgt
    51961 tattgacgga ggtctatcca aagcttatca aaaaacaacc gggattgctg gctactcatt
    52021 actatgcaat tcttacggtt tccaaatcgt gacacattat ccgttcttgc ctattgaaca
    52081 acaatttgca aaaaaatctg atcaaacgaa tgtaaaaaaa gtgatcgagc agcagctccc
    52141 acgcaaactt aatcggaata cgacaaaagg aatggaatta cagcaacaga ttactcaact
    52201 ccaaaggctg ttggattatc gaaaggacga ttgagaaaaa tgctagccaa cattgctcaa
    52261 tagactaaaa agcgactgca acaaaaggcg tgtcgcagtc tccttcggaa ttagctcaaa
    52321 aaacggtaaa atattagaag ctatttttct gtttatttct ctaatactca ggtcagggcg
    52381 ctgatttcac gacctcttcc ttaaacggtg ttctcctgcc aactcctcgg tcacaagtca
    52441 caatatccaa caaacatgag aagctgtttt ttgaaattgc tccttgtgac tcagagttga
    52501 acggcaggct cacgacctaa tcagaaaaac gattaaaatt cattcacaaa gaaatcgaat
    52561 aaacgctgtt cagatgaatc tacacgatga gaaagttccg gatgccattg gacaccaagg
    52621 attttgtgca tattatcttt actttggact gcttcaatga gtccatcgtt ggaaaaggcg
    52681 atggctttta acaaaggaga aagatctttg actgcctgat gatgatagga attcacaaag
    52741 tatgaatcag ggagaaggtc acttaataaa ctgtccgaaa cgattttgac ttcgtgagtt
    52801 gcaaactgcg gggcagtggg ctgttgttcg tgttttacgg accattttgg atataatgaa
    52861 agatcttgat aaagtgttcc ctccaacacg acattcaata gttgaagtcc tcggcagact
    52921 gcaaaaatcg gttttctttg tttcaatgct tccaagatca atgcctgttc gaattggtct
    52981 cgttgaatat ttgtttccgt cagttttgga tgagggtctt ccatgtaaag ttgcggacta
    53041 acatcctgac cgcctgcaag aatgagtttg tcgattttct caattagttg aggcgctgaa
    53101 tctggagaca tgactggcag aataagaggt aatccttgag catctaaaac tgcatccaca
    53161 aatccctgcg gtgtatagct tacttgattt ccttggaaag tatctgtcgc tcgaatcagt
    53221 tgattcccgg caatgccgat gattggtttc atttttattc cccctaaagt agactaattg
    53281 taacaaggat ttaaaaaaat ttaaactatg taagacttgc ccctatactt atctgttctt
    53341 gctgtgttac tatctgtaaa gagaattttc gtgtgaaatt catgacgata gttgaaaata
    53401 ttgcttaatt tgttataata aaaagtaagc agaaataaaa aggttgggcg taagcttcaa
    53461 ggatctagtt cttcaagggg tgagaaggac attttattga aggggcctgc ctatactaag
    53521 ttatctttag aggtacatac tttcaatgaa aaaagaaagc atgtacctct tttttaaatc
    53581 gaaaggaagt gaagcaagtg ttagaagctt tggaacaaat ccgaaaagca gaagaagaaa
    53641 atgagaagcg tgctcgaacc ctacggcagc agttgaagac ctatgcagaa gaaaaagagc
    53701 aagaattgga gataatccaa acgaacagtc ggcaacagct gaaacagttg gtagaagaaa
    53761 aagaacaatt cgaaaagcaa gaattagaaa aagaaaaaat gaaattgaaa gcagaagcag
    53821 acaaggtgca agcaactttg aaagaccaat atcaaaagca tcagagtcaa gcaatcggtg
    53881 caatcattga aagggtgaaa gaaacttatg gccgtcacta agatggaaaa agtgaccctc
    53941 atttcagata aaagaaatca ggagaggatt ttacaagcag tacaaggaat ccaaaatgtt
    54001 gaaatccggg atctgtatca aaatagaaca aacaaccagt gggtagaaaa atattttcca
    54061 gataccacga ttattgataa agaagcaaaa ttaagcgctt taggcaatcg gctgaaagac
    54121 atccaagaag ctatccaatt catacaacac cacggcgata gccgtcaaaa gaaactccat
    54181 ttgaaacgtc aagaactttc cttacaagac ttggaagcaa attattccga agaaaatttc
    54241 atgaataaat tagaagaagt ccttgattta aaagaaaagt gggaacagct aactgaaaaa
    54301 cgtgatcgtt tagaagaaga ggaagaatgg cttcttcatt ggcaacagct ggatattgct
    54361 ccaagtatgt atgcaagtaa gacgaccatt tttgagatgg ggactgtgag tgcagcaaat
    54421 tttgaaagtt tcaaggaaga actaagtaag cttgatgacg tttatatgga agaaatagat
    54481 tatacgccaa aacaagttta tctttcgtat gttgctttaa aaaaatcagt tgctgctata
    54541 gaagaaattg ctgggaaata cgggttcaat aaagaaacgt atccttatga acaaaatcct
    54601 aaagaaaaat tgcaagaaac caaacagcaa ttatccattg ttcacgagca gcaaaaaaag
    54661 ataacgacag cgttaggtca gtgcagcggc tatatccaag aatttgaatg ggtagaagaa
    54721 gtcacacttg ccgttgcaga acgggaaaaa gtaaaagaac ggtttatcca tgccacttat
    54781 ctgatcgttt tgcaaggttg ggtagatgca gaagaaaaac agaatttgat gcatgtgtta
    54841 aacgaaactg tatcagaaga agatatatac gttagtttcg aaacaccaaa ctcagaagag
    54901 attcagcagg aagtccctac taaattgaaa aaccatccgc ttatcgaacc ttttgaattg
    54961 ctgactgaaa tgtacagtct gccaaaatat gaagaagtag atccaacgcc ttggatgaca
    55021 ccattttatc ttgtcttctt cgggatgatg gtcgcagatg ttggttacgg attgctgatg
    55081 ctcattggga caatactagc tcaaaaacta tttgttttac cccgcgggat gaaacgtttc
    55141 gtaaaattct ttgagattct ctcggtacca tcgatcatct ggggattgat ctacagttca
    55201 tttttcggac aggcattacc aaaagaacta tttggtatcc gcttgccttt cccaatccta
    55261 tctacgacgg atgacgtcaa tacgattttg attttatccg tgattttcgg tctgatacag
    55321 atcttggttg gattattcgt ggcagcaaag gaaaatatca ggaggaaaga ttacttgagt
    55381 gctgtcagtg atggttttgc ctggcaaggg atcctgattg ggatcgttat tgcattgatt
    55441 ggtgtcatgt tgataaaaaa tacagcattt gtttatcttg gcgcggggct atcgatcatt
    55501 gctgctttat gtatcttaat cgtgccgatt atccaatcgc cgtctaaagt caagggtgct
    55561 gccaaaggag cgtataatct ttacggattg actggttata tcggtgattt agtcagttat
    55621 acacgtttga tggcactagg tatttcaggc ggaagtattg gcgcggcatt caatatgcta
    55681 gttgctttca tgccgccagc cgctcgattc agtgtaggga tccttttgat catcgcttta
    55741 cacgcactga atatgttttt gacactgtta agtgcctatg tccatggtgc acgattgcag
    55801 tatgtcgaat tttttggaaa attttataca ggaggcggac gtgcctttga cccgctgaaa
    55861 acggcggaaa aatacgtcaa catcaatcat aagaaaaaaa acaataaata attggaggat
    55921 ttttatacaa tgatggatta cttaattagt caaaatggtg gaatggtatt tgcagtatta
    55981 gcgatggcaa cagcgacgat tttttcaggg attggttcag ctaaaggtgt tggaatgaca
    56041 ggtgaggcag cagcagcgtt aacaacaagt caacccgaaa aattcggaca ggctttgatt
    56101 ctgcaattgc ttccaggtac gcaagggttg tatggattcg ttatcgcctt tttgattttt
    56161 atcaacttag gaaatgatat gtcagttgta caaggattga actttctagg cgcttctctg
    56221 ccgattgctt ttacaggttt gttttcaggt atcgcccaag ggaaagttgc agcagcaggt
    56281 atccaaattt tagcgaaaaa acctgaacat gctacaaaag gaatcatttt tgctgcgatg
    56341 gttgaaacat atgcgatttt aggtttcgtt atttccttcc tattagtatt gaatgcctaa
    56401 gcataaagga ggaaaagcag atgaacgcca ttgaaaaaat catctctcag atgaatgaag
    56461 cagcagagca agaaagagcg gcgttagagc aagaagagcg aatgaagatc gatcaaaact
    56521 ttgaacaaaa acgtacgcaa atcgaaacag aacatcaaaa acaaaaagaa aaacaaatcg
    56581 aattacttga aaaaaaatac cgtcagctta ggaatcgcca acaagtagaa gtacgacaag
    56641 aaaatttgaa tgcgaagcag gaatttttac gacgattgtt tgcagatgca atagctgaaa
    56701 tggagaattg ggatgaatct gaacaaatcc agtttatcaa aaattcattg tattcattac
    56761 ctttgacagg caaagtaaca ttcattgctg gagaaaaatc agcagcttat ctttctcaga
    56821 cattgctgga tgaatggaac aatgaacttc cctttatgat ggttttaagt gatgaaactg
    56881 ttgctgatca agcgggtttt ctgataaacg atcaaggagt tcagtataat tttcttttca
    56941 gcagccttgt acaagatatc caaggaacga tgagttttga gattgccaat caattattcg
    57001 aataaggagg gatcagtgta tggaatacca cgaactaaat cccttgattc gcggaagaga
    57061 gcttgaactt ttgtccaatg agacatttga acggatgatt caaacaaatt ctgtcgaagc
    57121 acttggtgaa atattgaaat ccacgattta cagtccatat atttatgacg gatatgaata
    57181 tgacttcgaa cagaatcttt taaaggagca gagccgtcta tttcgctggt taaaggaatc
    57241 agcaccggag cctgaaatcg tttggatcta tacgatgcgc tatactttcc ataatctgaa
    57301 ggttttgaca aaagccgaga tgacaaatca aaatctggac catttgtata tcgacgatgg
    57361 attttattca ttagaaacct tgaaggatgc cattcatacg caagtttcaa atgaactccc
    57421 tgaaagcatt atggacagta tacgagaagt acgtgattat tttgaagagt ctaccattct
    57481 tcagggaatt gacgtgattt atgatcgaca gtttttgaca gagcagcgac ggcttgccga
    57541 taagctagga tatcaagaat tgctgacaga agtcattgct tttattgact tgaccaatat
    57601 cacaacgatg gctcgaggaa tattgcagaa aagaacatat gggttcatga cgacggtact
    57661 ttccagctcg ggaagtatcg aaaaagaagt atttttgaac tttgtagata aagatatgga
    57721 agattacacg aattttctat tagagacaga ttatgctgat tttctgcgcc ctgtcatccg
    57781 tgagggaaat cttgatctcg taaagcttga acagttgaaa gatgattatc taagttcatt
    57841 gtatcaagaa gcacaaaccc aggctttcgg accactgcca ctactagcct ttttgaacgc
    57901 aaaagaggta gaaagcaaaa accttcgttt actggttatc ggcaaaagaa gccatttcga
    57961 accagaaaca atcagaaagc gggtgagaca agtctatgac gcataagatc ggtgtagttg
    58021 gcgacaaaga ttctgttttg ccattcaaat tgtttggctt tgatgttcgt tatggtacaa
    58081 ccaagcaaac cgttcgtcag gcaattgaag aaatggcaaa gaatgagtat ggcgtgattt
    58141 atgtcacaga gcaatgtgca gcattgagcc cagaaacgat cgaacgttac aaggaacaaa
    58201 tgatacctgc tatcgtattg attcccaatc atcaaggatc attagggata gggctggaag
    58261 aaattcaaaa aaacgtagaa aaagcagtag gtcaaaatat cttatagtat gaaaaggaga
    58321 tagaaatttt gcaaattgga aaaatcataa aagtctccgg acctcttgta atggctgaga
    58381 acatgtctga tgccagtatc caagacatgt gtctagtggg agatctagga gttatcggag
    58441 aaattatcga gatgcgtggc gatgtggcgt ctattcaagt atatgaagag acttctggaa
    58501 ttggtccagg cgaaccggtt cgctctactg gtgaagcttt atctgttgaa ttaggaccag
    58561 ggatcatttc acaaatgttt gacggaatcc aacggccatt agataccttt atggaaatca
    58621 cacaaagtaa tttcttaggg cgcggagttc aattgcccgc attggaccat gagaaaaaat
    58681 ggtggttcga accaacagta gaagccggag aaacggtgag tgctggagat gttatcggga
    58741 tcgtagaaga aacaaaagtt ataaaacata aaatcatggt gccaaatggg ataaaaggaa
    58801 cgatcaaaac aatcgaaaaa ggttcattca ctattgatga aacagtatgt gtcattgaaa
    58861 ccgaaaatgg tgacaaaaat ttgacgatgc ttcaaaaatg gccagtaaga cgtagtcggc
    58921 caatcaaaga aaaattaaac ccggatgcac ccatgatcac tggacagcga gtgattgata
    58981 cattcttccc agtgacaaaa ggaggagcag cagctgtacc tggtcctttt ggtgcaggga
    59041 aaacagtcgt tcagcatcaa attgcaaaat gggccgatgt ggacatggtt gtctatgtgg
    59101 gatgtggcga gcgtggaaat gagatgacgg atgttttaaa tgaattccct gaattgattg
    59161 accctaatac aggagaatcg ttgatggaac gtacggtatt gattgctaat acatcaaaca
    59221 tgccagtagc agctcgagaa gcttctatct atacgggaat cacaattgcg gaatatttcc
    59281 gtgacatggg ttatgacgtt gctatcatgg cagactccac ttcacgatgg gcagaagcac
    59341 ttcgtgagat gagcggacgt ttggaagaaa tgcctggtga tgaaggttat ccagcttatc
    59401 ttggttcacg gcttgcggaa tactatgaac gatctggaag agtcattgca ttaggatcag
    59461 aaggtcgcga aggaagtata accgcaatca gtgctgtatc cccgtctgga ggggatattt
    59521 cggaaccagt cacacaaaat acattgcggg tcgtaaaagt attctggggt cttgattcca
    59581 gtttagccca aaaaagacat tttccttcga tcgattggat acaaagctat tctctttatt
    59641 caacagaagt agggaagtat atggatcata tccttcaagc tgactgggca tccatggtca
    59701 ccgagggcat gcgtattttg caggaagaac agcagctgaa tgaaatcgtg cgtttggtag
    59761 ggattgattc cctttcagat aatgaccgtc tgacattaga agtggcaaaa tccattcgtg
    59821 aagattattt gcagcaaaat gcttttgatg atgtcgatac attcacttca agagagaaac
    59881 aattcaaaat gttgaagatg atcttgacct ttggtgaaga aggacgccgt gcgttgtcgt
    59941 taggtgctta tttaagtgag atcatggacg gtaccgtatc gattcgtgaa aggatcggac
    60001 gaagcaaata tatcccggaa acagaattag aaaaactaga tcaaattatt ggtgaaatca
    60061 agcaaacgat gcaaacgatc gtttcagaag gaggcatgag caatgattaa agaatatcgt
    60121 acgatcggtg aagtcgtcgg tccattgatg gcggttgaaa aagttgctgg tgtcaaatat
    60181 gaagaattaa tcgaagtccg tatgcaaaat ggcgagattc gtcgtggaca agtattggaa
    60241 gtccaagaag ataaagcaat ggtccaaata ttcgaaggaa caagtggtat caatttacgt
    60301 gactcttctg tacgattttt aggccatcct ttagaactag gtgtatcgga agacatgatc
    60361 ggtcgagtat tcgatggttt gggacgaccg aaagataacg gaccagagat cctgcctgaa
    60421 aagcatttgg atattaacgg agaagtaatc aatccagtag ctcgggatta tccagatgag
    60481 tttatccaaa caggtatctc tgcaatcgat catttgaata cattagttcg tgggcaaaaa
    60541 ttaccagtat tttctggctc tggtcttcct cataaagaat tagctgctca gatcgcgcga
    60601 caagcaacgg tactcgattc atcagatgat ttcgccgtag tctttgctgc tattggaatc
    60661 acttttgaag aagctgaatt tttcatggaa gattttcgtc aaacgggcgc aatcgaccgt
    60721 tctgtcatgt ttatgaattt agcaaacgac cctgcaatcg aacgtatcgc tactcctcgt
    60781 atggctttga ctgctgcaga atatcttgct tatgaaaagg gaatgcatgt tttggttatc
    60841 atgacagaca tgaccaacta tgcagaggca ttgcgagaga tttctgcagc acggcgagaa
    60901 gttccaggaa gaagaggata tcctgggtat ctttacacga acttagctac tttattcgaa
    60961 cgagcaggaa gaatccgcgg actaaaagga tcggttactc aaattccgat tctaacaatg
    61021 ccagaagacg ataaaactca tccaatccct gacttgactg gttatattac agaaggacag
    61081 atcatcttga ctagagagct ttacaagagt gggattcagc caccaattga tgtactcccg
    61141 tctctttcgc ggttaaaaga taagggaact ggacccggaa agacaagaga agaccatgca
    61201 gctacgatga atcagctttt tgcagcctat gcacaaggaa aacaggcaaa agaattagcc
    61261 gtagttttag gtgaatctgc tttatctgat gtagacaaga tctatgcgaa gtttgctgag
    61321 cggtttgaaa aagaatatgt caatcaagga ttctacacga accgttcgat tactgaaaca
    61381 cttgatctag ggtgggaact gctatcgatg ttgccaagaa ccgaattaaa acgaatcaaa
    61441 gacgacatgc tggacaaata tctgccagaa gggaagtgag ttctgatggc acgattgaat
    61501 gtgaatccga cccgcatgga attgactcga ttgaaaaaac agttgacaac tgccacaaga
    61561 gggcataaat tattaaaaga taagcaagat gaactgatgc gtcaattcat cctgttgatt
    61621 cgaaagaaca accagctgcg tcaagaaatg gaagagtcta tgacagaagc aatgtctgat
    61681 tttgtattgg caaatgcttc gatcacggaa cctttcatcg aagaattatt tgttttgccg
    61741 gcggaaaacg ttgatttgtc tatcgtagac aaaaatatca tgagtgtaaa agtacctgtg
    61801 atgaattttg aatatgatga acagctgaca gacacacctt tggaatacgg gtatttgaat
    61861 tccagtgccg aactggatcg gtcaatcgat cggtttacta ccctgcttcc aaaacttttg
    61921 gaactgacag aaatcgaaaa aacttgtcaa ctgatggcga gtgaaatcga aaagacacgt
    61981 cggcgagtca atgcattaga atatatgacg atccctcaat tagaagaaac gatctattat
    62041 attcgtatga agctggaaga aaacgagcgc gcagaagtaa caaggttgat caaagtaaaa
    62101 aatatgggta ccactgacta agtggtatct ttttttaaaa gaggaaaacg atgattaaac
    62161 aaagcaaaca aataaagaaa cgtttgccgg ctgttcagct tctcgcatta ggattttttc
    62221 tcctgatttt agttggcggc ggcctactta ccctaccatt ttttagtaat agtgggcatg
    62281 ggacaaattt tatcgatgcg cttttcactg caacttctgc tgtctgtgta acgggattga
    62341 caacattgaa tacagcagag cattggaaca gtgctggtca atttttgatc atgcttttga
    62401 ttgagatcgg cggtcttggt tttatgatgg ttccgattct atgctttgca ttgataaaga
    62461 agaaagtcag tctcagcaca cgtattatat taaaagaagc gctgaatcta gaagaagtat
    62521 caggtgtgat gaagctcatg ctttacatcc taaaattggc ctttgttatc caattgattg
    62581 gtacggttgc tttatctttt gtatttgtgc ctgaatttgg ctggttgaaa ggtatctggt
    62641 acagtttgtt ccatgcaatc tctagttttt gtaatgcagg gtttgatttg ttaggagaca
    62701 gtttagccaa tcaccagcaa aatgtttatt tgatcatggt ggtatctgct ttgatcattt
    62761 ctggtgggtt aggcttcatc gtatggcgtg atttactgga atatcatcag aaaaagaaga
    62821 tgacgattca ttcaaaaatc gcattaaccg ttacaggtac ccttttatta ggcggattcg
    62881 ttgtgttctt tttcacagag cgcaatgggg caacgctagt ggaagggaat atgtttcaac
    62941 gattggcaaa cacctttttt atgagtgtga cacctagaac ggctggatat tattcagttg
    63001 attacctcca tatgagccat gcaggtctga ttttaacgat gtttttgatg tacatcggcg
    63061 gaacatctgg gtcgacggcc ggcggattga aaacaactac gttaggtgtg ttgctgatcc
    63121 agatgcgttc gatgttgaaa ggacgtacga gagcggaagc atttggccgt accatccgac
    63181 aaggcgcagt gttgcgggca cttactttat tttttgttac gttgacgttg tgtgtagtcg
    63241 ccatcatgat tttatcggtg actgaaacga tacctaaaac ttctggcatc gaatatattg
    63301 cttttgaggt cttctctgcg tttggtacag taggtcttac gatgggattg acgccagact
    63361 tgactgcaat agggaaattg atcattatag cgcttatgta cattggacga gttggtgtat
    63421 tgactgtggt cttctctctg atgacaaaag cacaaagaac acaatcaaaa tacaaatatc
    63481 cagaagaaag tgtcatgatt ggataatttc atcttctgac agaaacctag tgatttagta
    63541 aaataagaaa ataaaataat ttttcttgct tcttggatac tttaagtgca aaatgttcgt
    63601 ttattttttg aggcactacc cgttgttgaa cctcgggtag tgcctctttt ttattgaaag
    63661 gaatcaataa gatttgattc ctttatttat caataggtta taattcatat ttttttattt
    63721 tttcttttac gatacttagt ccagaagcta aaatttcaag gttattagag gatagggaaa
    63781 ttcgaacgaa tgcatctggt tgcgcacgtg tagtaaagcg tctggaatga taaagtcgga
    63841 taccgttagc aagaaagtca ttctcaatta tcttagggtc aatctcagga tcaactggaa
    63901 gaattcgata gtaagggaca cgattcgttg gtttttctac tttgaaaaga gaatcaaaaa
    63961 gctgattggc tctttttgta agacttaatt tagtttctaa gatcctattt gcttcatttg
    64021 aatggaggat ttgagtgatg acttccacat ctaatccaga agttttcaca tttatattaa
    64081 aaattgcttg ttctatcttg ctttggacat gtactggaac tacaagataa gcaactcgca
    64141 atccagaaca aataaatttt gtcattccag ctaagtagat tgtttggtta ggtaataaat
    64201 tttggaaagg aggaatgatc gtatgttgat agtaagttgt taaaaatgaa tggatatcat
    64261 cttcaataac ccatagacct tctttttcaa tcacttcggc taattgctct cgtcttttgt
    64321 gagagatttg aaaccctata ggattattac aagaaggcat aagaaagaca ccatgaatct
    64381 tcttttttct acattcctga atcaatatat caggtcgcat tccttgctcg tcaaaatcaa
    64441 ttggaacgat ctctaaatga tataactgag ccaattcaat gaaattagcg tatgtatatc
    64501 ggtctgttgc tattctatca cctggagaga aaagtgcaga aagtgtgata gccaatccat
    64561 tttgaacacc ggaaacaata gatagagatt ctaaggacgg aatgactcct tgagttttta
    64621 accaatttgc tgcagtagtc aattgaaaat ttgtgccttt gggatcttgg taattaagca
    64681 aaccaacaag atttgggtta ttactagttg attgaataaa cgggacaacg aatttattac
    64741 attcttcaaa agaactaacc agaccaaaat caatgatatg ttcggttact tcattggtag
    64801 agatggtgat ggactcaaag acattgggag aaataaatgt gccacttcca atattcgtat
    64861 acagcaaacc tttctcaatt ccatatttat aagccttgcc aacggtagta aaatttaaat
    64921 ctaaataatc agctagctct ctttgggagg gtaactgagt gtttttctgt aatttattct
    64981 ttaaaatatc ttgttctaat tgttgaatca acgatcgata ataaggacgc ttcaattttt
    65041 gtttgtctgg tcgccaagtt aacgggtaat tgtcataaga attaataggc attttctcca
    65101 ctccttaaaa ttgtatacca tacaatttta ccattgattg tattgatttt agaagttaat
    65161 cttttgtgag gagggaattg tctatgaaaa ttattaattt tgaggaagta gtagcttctt
    65221 ttagttttga agaagcgatt caagtaatga aaaaatgttt ttttgattat gaaagtggta
    65281 aaatcagcca aaatcctcga ttggtggaaa ttttacctga cggaaaaggc aaaaatatgt
    65341 ttgcgatgat gcctgcttat ttgggggaga acagatattt tggcacaaaa gtcattaccg
    65401 cttttccgga taatcatcag tatcatttac cttctcattt aggagagatt ttcttgtttg
    65461 atgctcaaaa cggactaccc aaagcattga taaacgcaaa tgcagtcacc tggattcgaa
    65521 cagctgctgt atctgcgctg gcaacagatt acttagcaaa tccgcatgca gaaacacttg
    65581 ctttaattgg agctggtcaa caagcattgt cgcatttgac tgcgatttct accattcgtc
    65641 ccatcactac agtgtttgtt tacgatattt tagaagaaag acgaaatact tttatcgcta
    65701 aagctaaaaa acagtatcct aaaataacgt ttataaatgc aatatcagta gaagatgcag
    65761 tacgtccagc ggaaatcatt tgtacgttga cctctagtaa agaggcattt cttcaaaaag
    65821 agtgggtaag ggaagacgca catatcaatg ctgtgggcac atttactcct gatacaagag
    65881 agataagtac atcgttgatg gaaacttctc aaatctatgt agatgattac tcagctgcct
    65941 taacagaaag cggggacctg cttatagcca tggcagaagg agatttagag gaatcttgta
    66001 ttgttggtac attaggcgaa ttagtttctg agaaaaaaac attttataaa ggagaatcga
    66061 ggccgacgat tttcgacgca gttggtctag ctattgaaga tctttgttgt gcagaatata
    66121 tttataacaa attagagaaa ggggctttaa aatgaactta gattattacg tagttgatgc
    66181 atttgcagat gaggtgttca aaggaaatcc agcagcggtt tatgtgctag aagaatggct
    66241 gccagaaggt acgatgcaaa aaattgccat tgaaaacaat ttatctgaaa cagcatttac
    66301 agtgaagaaa aaccaagaat ttgagcttag atggtttacg ccagatcgag aaatcgatct
    66361 ttgtggtcac gcgacattag caacagcatt tgttttattt aactattaca aaatacctga
    66421 tgaaacgatt aaattttcta ctcaaagcgg taatctattt gtgacaagaa ttaaagatta
    66481 ttattacatg gattttccta gtattatgcc gaaaaaagtg ccgatattgt ctgaatatga
    66541 agaagcgatt ggggctaaga ttaaagaagc ctacttagca cgtgatttat ttttcgtatt
    66601 agaagatgaa aaaacggtgg ctaaattgca gccagatttt acagctatca aaaattttga
    66661 attaggaata ggagtcatcg tgacagcaga aagtgtgcaa aaagactttg tttctagaac
    66721 cttctttcca aaattaacaa tcaatgaaga tccagtatgt ggatcagcac attctaatct
    66781 tattccttat tgggcagaaa gaatgaataa aacaaaattg tcagcttatc aaatgtcatc
    66841 aaggggcggg gatctagaat gtgaattaaa agaagatcgt gtgataatag gaggaaaagc
    66901 agttttgttt tctaaaggta cctcgtttat ttgatttctt tcaaatgaat taaaaataaa
    66961 ttggtggaga gtggcaacct tatagctaca atggttgtta aaaaaagacg caaacttttt
    67021 caatttaatg aaaagtttgc gtctcatttt aattattggt gtttgttttt tattcaatca
    67081 agagtaaaat gccccatact aactatcctc ggatgacttc gattttataa ccatcagggt
    67141 caatgacaaa gtagtaagaa ggcgcagtgc caggtaatcc ttttaagtca gtaatctcaa
    67201 gaccggcagc tttatgttgc tcgtgtaatt tttctagatc atcagctgct atagcgatat
    67261 gaccatagcc gtctcctaga tcatacgcac catgatcata gttgtaagtc aactctaatt
    67321 catagtcatc cccaggaagt gtcaaataaa caagtgtaaa tttgttttct ggaaaatcac
    67381 ggcggcggct ttcttcaaaa ccaaatgcct tttgataaaa ttcgattgat gcttcaagat
    67441 ctttcactcg aacacatgtg tgagccattt tcatagattc atctctcctt ttccttttac
    67501 tcaatcataa caaaaagaag taaggaaata gttgatttgt gcttgttcat gagaaatttc
    67561 tcatgtaaaa tggatagaaa ataaaactgt taaggtggaa actattcatg gataaaccaa
    67621 tttttggtga aaaaaagcaa tttgctgatt atcaaacccg ttatgcagca tatattgtca
    67681 tagaaagaga tcagcagatt gcagtaatcg aggctccaaa tggtgctttt tttcttcctg
    67741 gcggggaaat tgaaggagaa gagacgaaag aagaagcgat tgtccgtgag ctgatagaag
    67801 aaatgggaat tgtggcagaa atttcttctt atctgggaga agctgatgag tatttttact
    67861 cgaattatcg tgcaacttat tattataatc caggatattt ctttgttgct gattcttgga
    67921 agcgtatagg agaaccaact gaaaagacca ataaaatttg gtgggtgagc cctcaagaag
    67981 cgcttgtgaa attgaaacgt ggtagccacc gatgggcagt cgagagatgg atcgaaaaaa
    68041 ataaaaactg aaagaaagcg agtagatcag ctttctctca gtttttttat ttattacagt
    68101 acatattttt tgattgcttc agctacaccg tgctcgtcgt ttgatgaagt gatgacattt
    68161 gctgcttctt tgacaagaga aacggcattt cccattgcta ctcctagacc agcatattca
    68221 atcatggata agtcgttctc gttatctccg atagccatga tttcatcttg atcaatacca
    68281 aggtgctgtg cgagattcgc aactgccgca cctttacttg cttctttatt tagtacttcg
    68341 aagtaatagg gcgcactttt gaccgttgtg tatttatcac ggaaagtttg cggtaaacgc
    68401 gcaattgctg catctaaaat ttctggttcg tcgatcatca tcattttgac gatgttcatg
    68461 tctggtgtca tttcttcagg tgttcgatat tttagcggca tcttgactaa acttgcttcg
    68521 tggatcgtat aagcgctgat atctcgatta ggcgtgtaga tcgctttgtc atcgatcgaa
    68581 tggagatgac tgccgacacg gcgtgccatc acttcgatct ctaggaaatc ttcatgtgtc
    68641 agtccgtgac gggcgatgat tttacctgat ttcgtttgtt gtacgagtgc cccgttataa
    68701 gtaataacgt aatcattatc ttgaaaaagg ttcaactcgt ttaactgttc ctggacacct
    68761 ggtaagggac ggccagtgca taaaacgatc gaaacacctt ttgcaacagc agcggcaatc
    68821 gcttctttta cttcttttgt tacttctctt ttctcattta ataaagtacc atcgatgtcg
    68881 atagcgacta gtttgataga cataatttcc tccaatatat tttaatttat ttcaatcaat
    68941 gcgccattac ggatatggct ggcaaattct tgataagttt cgtcaaataa atcataatga
    69001 tcacgcaagg acggatcaac catttctttt ggaaagaaaa atcgctcatc tccttgttct
    69061 tgtcccgcta atgcagcaac aagctgacta acctgtgata attctatcaa agttccgtca
    69121 tttcgcaaaa tctcgatttg tgtgcgatgt ctgccttgtt ctgggcgata aaaatcataa
    69181 ggtagatcgt agctagaatt cacagcagtg taataaacag gattatatcc gaccttttct
    69241 actaacaaag tgagttcttg gactaatttt gaatcccggt tgccagaaaa agtagcagat
    69301 ttcagtggtt tacgattcaa aaaacgttta gctaaatcat ttaaaatagg atcagcagat
    69361 tcagaccatt gagtaaagta agtgcccaag accccatcat caagtttgag gtattcttct
    69421 aaagtaaagc ttccgtcaag aaatggcaga agtaattgag aatggagttc aaaactgctg
    69481 gcatcttctt gatacaactc gtgtgcgcga tgaagcagat gctccaaaat tacttccata
    69541 ccacgggaaa caggatggaa gtacacttgc acatacattt gatatcggct aacgatataa
    69601 tcttccactg catgcatgcc actcatggaa aaagcgatcc cgccttcata aggacgaatc
    69661 acacgtaata ttcgggtaag atcaaatgtt ccatattctg ttccagtgaa ataagcatcc
    69721 cgaagcaaat agtccattcg atctgcatcg atctgactag aaatcatttg gacaacttgt
    69781 gggttggggt aagttttttg gatcacacta gctacctttt caggaaaacc ttcttctact
    69841 cggttaagaa tctgatacac ttctgtttct ggagaagtga tgatctctac agtgatggct
    69901 tcatgattcg tatggaagat atgttcaaat gtatgagagt aaggaccatg accgacatca
    69961 tgcaacagtg ccgcacataa agtcaccagc cgttcctgat catcccagcc tccgttgcct
    70021 attttttctt ttgaaaaatt tcgagaaaag atatcgcaga ttcgtcttga aatctcataa
    70081 acgcctaatg aatgagtaaa acggctatgt tctgctccgt gaaaagtgaa agaagaagtg
    70141 cctaattgtt taatgcgtcg tagacgttgg acttctttcg aattgatcaa atctaagatt
    70201 acttgatgct ggacatggat atagttatgg actggatcac gaaaaacttt ttccataggc
    70261 agcatttgat atttataagg ggttgtcata atgtagctcc tttatgttat tacgttgttc
    70321 cattctctgg atttggttgt tccattgctc cagctggatt tcttttttta gccagtcggt
    70381 agaatcctgt aaatcacttg cagaatacag cttttcaaaa gcttttgcaa atgcttcttt
    70441 gacttctttt acaaaaaggg gttggtcaag cagttcttca agtgttgcca ttgtagcagg
    70501 atccactgca ggatagccat ttgtaccaaa gtcttctttt aaagcagatt gataaaatcg
    70561 cttcatgact tgcccgcgtt tttcctgatt accgttgaca cttaagtaca tcataacaga
    70621 aatcccattt ttgatccgac gctgagcgat accggcaaat tttttgccat ggatgcttaa
    70681 atcataagtt ccaggacaat aagaatgggt gatttcgaat gcttgtatcg gtgcctttgg
    70741 aaaagcgtat ttagtcaata ggagcatttg ctcgtaagcg tcatctatgg ttattttttt
    70801 tgtgtcactt tgggggaaaa tcaaagaaat attcaaaatt ccttcatctg atacgatccc
    70861 taatccacct gaatttctga tcactggagc gtaacctgca tctttcagtg tttggatccc
    70921 gtcacttaga aaaggagttc gaacatcctt catacctaag atgactgtgc agtcatattg
    70981 ccagaaatgt aaaaagattt gctggtgttc tttagaatat tccgttaata tatctgttaa
    71041 tgcaaaaggg gaaaagtcgt ttttttgata
//
LOCUS       DACDKM010000004        70100 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20834_2016
            SAMN12640370-rid9891663.denovo.004, whole genome shotgun sequence.
ACCESSION   DACDKM010000004 DACDKM010000000
VERSION     DACDKM010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640370
            Sequence Read Archive: SRR10040891
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 70100)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 70100)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742835.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742835.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:04:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,012
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 29 of 75
            Pseudo Genes (incomplete)         :: 33 of 75
            Pseudo Genes (internal stop)      :: 19 of 75
            Pseudo Genes (multiple problems)  :: 5 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..70100
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640370-rid9891663.denovo.004"
                     /isolate="E20834_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(28..1593)
                     /gene="guaA"
                     /locus_tag="IV334_000244"
     CDS             complement(28..1593)
                     /gene="guaA"
                     /locus_tag="IV334_000244"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HAP8942344.1"
                     /translation="MTNVADMTNVEKIIVLDYGSQYNQLITRRIRDLGVFSELLSHRI
                     TAEEVKAMNPKGIILSGGPNSVYDENAFGIDPEIFNLGIPVLGICYGMQLITYKLGGS
                     VEPAKNREYGKALLEVTSDQAVLFKETPKQQTVWMSHGDLVTKVPEGFETVGTSKDCP
                     IASIQDTTRNFYGIQFHAEVRHSEYGIELLRHFAFDVCHCEGNWTMDNFIDMQVEKIR
                     EQVGDKKVLLGLSGGVDSSVVGVLLQKAIGDQLTSIFVDHGLLRKGEAEQVMESLGGK
                     FGLNIIKVDARDRFLDKLKGVSDPEQKRKIIGNEFVYVFDDEATKLAGEEGVSFLAQG
                     TLYTDVIESGTETAQTIKSHHNVGGLPEDMQFELIEPLNTLFKDEVRELGTQLGMPDS
                     IVWRQPFPGPGLGIRVLGEITEDKLEIVRESDAILREEIAAAGLDRDIWQYFTVLPGI
                     RSVGVMGDGRTYDYTVGIRAVTSIDGMTADFARIPWEVLQKISVRIVNEVAHVNRIVY
                     DITSKPPATVEWE"
     gene            complement(1652..2575)
                     /locus_tag="IV334_000245"
     CDS             complement(1652..2575)
                     /locus_tag="IV334_000245"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I pantothenate kinase"
                     /protein_id="HAP8942345.1"
                     /translation="MNDPMNYYRVAREEWREFYRNGQAPLTQDELDNIKSLNDRISMQ
                     DVRDIYVPLAHLIYLYMKEFESLFLSKGLFLHKYVEVPPFIIGIAGSVAVGKSTTARL
                     LQTILSRTFKRRNVQLITTDGFLYPNRILKERGIMDRKGFPESYDMEMLIDFLNRVKS
                     GQEEIKVPVYSHDVYDIIPDKYELIQQPDILIVEGINTLQLPANQQIYVSDFFDFSVY
                     VDADPVLIEKWYLDRFGALLDTAFQNPENYYYRFAIGDREEAFKMAKDVWKNVNLKNL
                     QEYILPTRGRADIILHKTENHIIDSIFMRKY"
     gene            2771..3376
                     /locus_tag="IV334_000246"
     CDS             2771..3376
                     /locus_tag="IV334_000246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAP8942346.1"
                     /translation="MTNHYYSERPDTAHDFEQWAFELKGRTFQFVTDSGVFSRETVDY
                     GSRVLIDAFDWEELPDEGKILDVGCGYGPIGLALAFATQRFVEMVDINERAVSLAQGN
                     AKRNQIEHVDIHQSNIYEAVHEETYAAIVSNPPIRAGKKVVHEILTGAYPRLKKGGTL
                     TIVIQKKQGAPSAQKKMEETFGNAEIVTKDKGYYIIKSVKE"
     gene            3684..4700
                     /locus_tag="IV334_000247"
     CDS             3684..4700
                     /locus_tag="IV334_000247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAP8942347.1"
                     /translation="MKLTIRDIAEMAGVSVTTVSQILNNKGSRFSDKTRKKVLSIVNE
                     HHYKPDYFASNVINRHSKTIGMIVPDVTDFFFSKLIEGVESYLNPLGYVIMLCNSRHS
                     QENEIKYLQELSHRSVDGILLATPNILPEKYALDSGFYQKMPIILIDRGLNRRDNGRL
                     IVKEYEGAYQAVSLFIENGHTKIGMLKETTGYYQLEERFNGYRHALKDHGLPFNGKFV
                     EHGELTVQGGYTASKKLLKHKDITAIFCGNDAMAIGCYQAIDELGKKIPEDISVIGFD
                     GLKLSEYMIPQLTTVQQPSFDIGFYAARFLIDTIEFPQRKVPNKVFETKLIIRESVKV
                     LTKG"
     gene            4841..6142
                     /locus_tag="IV334_000248"
     CDS             4841..6142
                     /locus_tag="IV334_000248"
                     /EC_number="2.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine-nucleoside phosphorylase"
                     /protein_id="HAP8942348.1"
                     /translation="MRMVDLIEKKRDGNELSKEEIEYIVTNYTNGKIPDYQVSALLMA
                     IFYQDMTNEEITNLTLAIANSGDVIDLSSLEGIKVDKHSTGGVGDTTTLILAPLVASV
                     GVTVAKMSGRGLGYTGGTLDKLEAIPGFQIELSDEEFVRIVNESKVAVIGQSGNLAPA
                     DKKLYALRDVTATVDSLPLIASSIMSKKIAAGADAIVLDVTTGDGAFMKNIEDARRLA
                     KTMTSIGKLANRETVAVISDMSEPLGEAIGNSLEVVEAIETLQGNGPEDLVEMCYALG
                     SQMVVLAGKAKTTDEARTLLQEALESGKALAKFKEMIQNQGGDPAIVEHPERILTARY
                     TMELPAKQSGVVSKIVANELGIAAMMLGAGRKTKEEDIDHAVGLKLHKKIGDTVTKGE
                     SLLTIYSNDEEITSVIELLYKNIEIGESAMKPTLIHDIITE"
     gene            6188..6850
                     /gene="deoC"
                     /locus_tag="IV334_000249"
     CDS             6188..6850
                     /gene="deoC"
                     /locus_tag="IV334_000249"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HAP8942349.1"
                     /translation="MELNRMIDHTILKADASKEAVMQIIEEAKKYHFYSVCINPAWVA
                     LAKEQLQGEPVAVCTVIGFPLGANTPETKAFETTDAINNGADEVDMVINIGALKSKDD
                     QQVQKDIEAVVEAAKDRALVKVIIETSLLDREEIIRACEIAKAAGADFVKTSTGFSTG
                     GAKVEDVRLMRETVGPEMGVKASGGIHNEEEAMAMIEAGATRIGTSAGVAIVSGSTGE
                     GY"
     gene            6871..7269
                     /locus_tag="IV334_000250"
     CDS             6871..7269
                     /locus_tag="IV334_000250"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HAP8942350.1"
                     /translation="MAELKQEWINTAIEALDKAYVPYSHFPVGACLVTESGKIYQGIN
                     IENASFGLTNCAERTAFFKAVSEGEKTFTHLVVAGHTPDPISPCGACRQVMAEFCSPD
                     MPVTLVGDNGVTKATTVRALLPYAFTEKDL"
     gene            7335..8417
                     /locus_tag="IV334_000251"
     CDS             7335..8417
                     /locus_tag="IV334_000251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HAP8942351.1"
                     /translation="MKKAKLFGLGAVALAAGLFLGACGNNGSTDSSGGKSSSDTTTAA
                     LITDTGGVDDRSFNQSAWEGLEKWGKDQGLSRGNDGFQYFQSSNESDYIPNIDQALNA
                     GFKTIFGIGYKLKPAIQEQATNNTGTNFVIIDDVIDGLDNVVSATFKDNEASYLAGVA
                     AAYTTETNVVGFIGGVKGEVIDRFDAGFKAGVDAGAKELGKEIKVLNQYAGDFSAPDK
                     GRSIAQGMYAQNADIIFHASGGTGNGVFQEAKSLNESGDKKVWVIGVDRDQSDEGEYT
                     LNGEKKNFTLTSTLKAVGTVVEDLAQKSADGKFPGGEHTVYGLKEDGVGLTEGQLSDE
                     AKKAVDEAKEKIISGDVKVPETPEEN"
     gene            8629..10191
                     /locus_tag="IV334_000252"
     CDS             8629..10191
                     /locus_tag="IV334_000252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP8942352.1"
                     /translation="MVQENIVIEMQNITKEFGTFKANDNINLQVKAGEIHALLGENGA
                     GKSTLMNVLSGLLEPTSGKILMRGKEVKITSPTKANQLGIGMVHQHFMLVDAFTVTEN
                     IVLGSEPSRAGMLDHKKARKEIQKVSEQYGLSVNPDAYVRDISVGMEQRVEILKTLYR
                     GADVLIFDEPTAVLTPQEIDELIVIMKELVKEGKSIILITHKLDEIKAVADRCTVIRR
                     GKGIGTVNVKDVTSQQLADMMVGRTVSFKTMKKEAKPQEVVLSIENLVVKENRGLEAV
                     KNLNLEVRAGEVLGIAGIDGNGQSELIQALTGLRKAESGHIKLKGEDITNKKPRKITE
                     HGVGHVPEDRHKYGLVLDMTLSENIALQTYHQKPYSKNGMLNYSVMNEHARELIEEYD
                     VRTTNELVPAKALSGGNQQKAIIAREVDRDPDLLIVANPTRGLDVGAIEFIHKRLIEQ
                     RDKYKAVLLISFELEEILNVSDRIAVIHEGEIVGIVDPKETSENELGLLMAGYTLEEA
                     RKELEKGAGAHE"
     gene            10184..11317
                     /locus_tag="IV334_000253"
     CDS             10184..11317
                     /locus_tag="IV334_000253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP8942353.1"
                     /translation="MNDRSEKFRNLLVPVLSVVLGLILGAILMAAFGFQPIQGYSSML
                     DAALGSQRGIGETLRQATPLIFTALGFSVANSAGFFNIGLSGQALCGWIVSVWVALAF
                     PDLPKVVLLPLCVILGALAGAMAAAVPGLLRAFFGTSEVIVTIMMNYILLYVSTFALH
                     EVMPESYRSSLDSSNMISQNASLRLPWLTQAFGGSRVNSGLFLAIIALIFVWLLMKKT
                     TLGFEIRSVGLNPFASEYAGMSSKRTIVLSMVISGALAGLGGVVEGLGTYQNFFVQTT
                     SLSIGFDGMAVSLLGSGSAIGILLSAILFSILKIGGLGMQTGAGVPFEIVNVSIALII
                     FFVGINFLIRFVMAKVFQGKKQEEIVATIESKPTNQNHEGGEL"
     gene            11317..12273
                     /locus_tag="IV334_000254"
     CDS             11317..12273
                     /locus_tag="IV334_000254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP8942354.1"
                     /translation="MSTIELIALILTSTLVYSTPLILTSLGGTFSERSGIVNVGLEGI
                     MVMGAFSSIVFNLTMSPSLGSMTPWIAILVGGVVGILFSLIHAVATVSLRADHIISGT
                     VINLMAPALGVFLIKAWYGKGQTDNITQNLGYFSFPGLADIPVIGPIFFKNTFLPAYC
                     AILIAVLAWVIIFKTKFGLRLRSVGENPQAADTLGINVYAMRYSGVLISGLLGGMGGA
                     VFAQSISGNFSAGTIVGQGFISMAAMIFGKWNPLGAMGASLFFGFAQSLSIIGAQLPV
                     ISSIPPVLLQIAPYLLTIIVLVVFLGKASGPKANGKNYIKSK"
     gene            12372..13538
                     /gene="deoB"
                     /locus_tag="IV334_000255"
     CDS             12372..13538
                     /gene="deoB"
                     /locus_tag="IV334_000255"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HAP8942355.1"
                     /translation="MFKRVHLIVMDSVGIGEAPDADKFGDVGSDTLGHIAKEAGLNVP
                     HLEQLGLGTIRPLEGVESVKDHQGYATKLEEVSVGKDTMTGHWEIMGLNIKTPFRVFP
                     NGFPDDLIKQIEEFSGRKVVCNKPYSGTAVIDDYGEHQMKTGDLIVYTSADPVLQIAA
                     HEEIIPLEELYRICQFVRDITKDDPYMIGRIIARPYVGEPGDFTRTSNRHDYALNPFG
                     HTVLDSLKEAGKDVIAVGKINDIFNGQGITESVRTKSNMDGVDQLLKVMKNDFSGISF
                     TNLVDFDALYGHRRDVVGYAHAIEEFDLRIPELLEAMEEDDLLMITADHGNDPTFTGT
                     DHTREYVPLLVYSKKMKEQGALPQGYYSDISATIAENFSVSSTENGESFLKLLK"
     gene            13562..14380
                     /locus_tag="IV334_000256"
     CDS             13562..14380
                     /locus_tag="IV334_000256"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HAP8942356.1"
                     /translation="MTKLSEMLQETTQFILDKGVKEIDFGLILGSGLGELADEIEDAV
                     MISYDEIPFFPVSTVVGHAGKLVYGSLAGKKVLAMQGRFHYYEGHSMQTVTYPVRVMA
                     GLGAHSVIVTNACGGVNESFVPGDLMLITDHINFTGENPLIGPNEDAMGPRFPDMSQA
                     YTPDYQATAKAAAEKLGLDLKEGVYMGYSGPTYETPAEIRMSRTIGADAVGMSTVPEV
                     IVAAHSGLKVLGISCITNLAAGMQANLSHEEVVETTQRVKQSFKALVKETLTLL"
     gene            14397..15104
                     /gene="deoD"
                     /locus_tag="IV334_000257"
     CDS             14397..15104
                     /gene="deoD"
                     /locus_tag="IV334_000257"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HAP8942357.1"
                     /translation="MSVHIEAKEGEIADKILLPGDPLRAKYIAETFLEDPVCYNQVRG
                     MLGFTGKYKGERISVQGTGMGMPSATIYAHELIQSYGVKKLIRVGTCGALSKDVHVRD
                     LVLAQGAATSSSMIEKNFQAFHFPPISDFNLLLKAYEIAKEKGYTVHVGNVLSEDSFY
                     KDDLTETFQLAELGVLGVEMEAAALYYLGAKYHVQTLSLMTVSDHLITGEETTAAERQ
                     STFNEMIEVGLETAIAE"
     gene            complement(15402..16088)
                     /gene="gpmA"
                     /locus_tag="IV334_000258"
     CDS             complement(15402..16088)
                     /gene="gpmA"
                     /locus_tag="IV334_000258"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HAP8942358.1"
                     /translation="MPKLVFSRHGLSEWNALNQFTGWADVNLAPEGIEEAKEGGRKIK
                     EAGIEFDVAYTSVLTRAIKTCNLILEYSDQLWVPQIKSWRLNERHYGKLQGLNKKETA
                     EKYGDEQVHIWRRSYDTLPPLMEETDEGSAANDRRYAMLDKRDIPGGENLKVTLERAL
                     PFWQDEIAPALLDNKTVLVAAHGNSLRALAKHIEGISDEDIMDLEIPTGQPLVYELND
                     DLTVAKKYYL"
     gene            complement(16260..16841)
                     /locus_tag="IV334_000259"
     CDS             complement(16260..16841)
                     /locus_tag="IV334_000259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAP8942359.1"
                     /translation="MKIIGYARTTINDVDLTTQVDQLSDFGCQEIFHETYEVEQNDST
                     AVHLESVIADMQQGDALVICQLHRLGKSTRQLTELTQLFKDRGLHLISLDEGIDTREM
                     GKIYFQLMENLANMECALIKERTLVGLDEARKKGKIGGRPKIDAKTIKKIRRLYYEKK
                     ETIQFISSKCGVSVGTCYKYINLPEKEIEQLCI"
     gene            complement(16974..17657)
                     /gene="rpiA"
                     /locus_tag="IV334_000260"
     CDS             complement(16974..17657)
                     /gene="rpiA"
                     /locus_tag="IV334_000260"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HAP8942360.1"
                     /translation="MNLKQLAGIEAAKFVKDGMIVGLGTGSTAYYMVEEIGRRMREEG
                     LRITGVTTSNATKEQAEKLGIPLKSIDEVPVVDLTIDGADEISADFQGIKGGGAALLF
                     EKIVATYSKETIWIVDSSKLVDKLGKFPLPVEVIPYGSQQLLHIFEEKRFQPVLRTDE
                     NGEVLTTDGGHYIIDLHLEVIEQPESLATYLDELVGVVEHGLFLNMVSKVVVGSEKGV
                     EILDAIRRK"
     gene            17976..18389
                     /gene="rpsL"
                     /locus_tag="IV334_000261"
     CDS             17976..18389
                     /gene="rpsL"
                     /locus_tag="IV334_000261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007085295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HAP8942361.1"
                     /translation="MPTINQLVRKPRKSKVEKSNSPALNKGYNSFKKTQTNVNSPQKR
                     GVATRVGTMTPKKPNSALRKYARVRLSNLIEVTAYIPGIGHNLQEHSVVLLRGGRVKD
                     LPGVRYHIVRGALDTAGVNDRKQSRSKYGTKKPKA"
     gene            18473..18943
                     /gene="rpsG"
                     /locus_tag="IV334_000262"
     CDS             18473..18943
                     /gene="rpsG"
                     /locus_tag="IV334_000262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HAP8942362.1"
                     /translation="MPRKGPVAKRDVLPDPIYNSKLVTRLINRVMVDGKRGIAANIIY
                     NAFDLIKESTGNDPLEVFEQAMKNVQPVLEVKARRVGGSNYQVPVEVRPERRVTLGLR
                     WVVNYARLRGEHTMEERLAKEIMDAANNTGASVKKREDTHKMADANRAFAHYRW"
     gene            19037..21124
                     /gene="fusA"
                     /locus_tag="IV334_000263"
     CDS             19037..21124
                     /gene="fusA"
                     /locus_tag="IV334_000263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HAP8942363.1"
                     /translation="MTREFSLQNTRNIGIMAHIDAGKTTATERILYYTGKIHKIGETH
                     EGASQMDWMEQEQERGITITSAATTAQWKGHRINIIDTPGHVDFTVEVERSLRVLDGA
                     VTVLDAQSGVEPQTETVWRQATTYGVPRIVFANKMDKIGADFLYSVSTLHDRLQANAH
                     PIQLPIGAEDDFTGIIDLVTMKAEMYTNDLGTEIEETEIPEEYRELAEEWREKLVEAV
                     AETDEELTLKYLEGEEITEAELKEGIRRATVNVEFYPVLCGSAFKNKGVQLLLDAVLD
                     YLPSPLDIPAIKGIDPKTDEEVERPADDSAPFSALAFKVMTDPFVGRLTFFRVYSGVL
                     QSGSYVQNATKGKRERVGRILQMHANSRSEISEVYAGDIAAAVGLKDTTTGDTLCDEK
                     SLVILESMEFPEPVIQVAIEPKSKADQDKMGVALQKLSEEDPTFRAETNVETGETIIA
                     GMGELHLDIIVDRMRREFKVEANVGAPQVSYRETFRAGTQAEGKFVRQSGGKGQYGHV
                     WIEFTPNEEGAGFEFENAIVGGVVPREYIPAVETGLKDAMENGVLAGYPLVDIKAKLY
                     DGSYHDVDSNETAFRVAASMALRAAAKKANPVILEPIMAVEVVIPEDYLGDVMGHVTA
                     RRGRVEGMEARAGGQQVVRAMVPLAEMFGYATTLRSATQGRGTFTMTFDHYEDVPKSV
                     QEEIIKKNGGKAE"
     gene            21296..22483
                     /gene="tuf"
                     /locus_tag="IV334_000264"
     CDS             21296..22483
                     /gene="tuf"
                     /locus_tag="IV334_000264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HAP8942364.1"
                     /translation="MAKEKFDRSKPHVNIGTIGHVDHGKTTLTAAITTVLSKKNGGQA
                     MAYDQIDGAPEERERGITISTAHVEYETDTRHYAHVDCPGHADYVKNMITGAAQMDGA
                     ILVVSAADGPMPQTREHILLSRQVGVPYIVVFLNKVDMVDDEELLELVEMEVRDLLTE
                     YEFPGDDVPVVAGSALKALEGDASYEEKILELMAAVDEYIPTPERDNDKPFMMPVEDV
                     FSITGRGTVATGRVERGQVRVGDEVEVVGIAEETSKTTVTGVEMFRKLLDYAEAGDNI
                     GALLRGVAREDIQRGQVLAKPGTITPHTKFSAEVYVLTKEEGGRHTPFFTNYRPQFYF
                     RTTDVTGVVELPEGTEMVMPGDNVTMEVELIHPIAIENGTKFSIREGGRTVGAGVVTE
                     IKA"
     gene            22890..23198
                     /gene="rpsJ"
                     /locus_tag="IV334_000265"
     CDS             22890..23198
                     /gene="rpsJ"
                     /locus_tag="IV334_000265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003046044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HAP8942365.1"
                     /translation="MAKQKIRIRLKAYEHRILDQSADKIVETAKRTGASVSGPIPLPT
                     ERSLYTIIRATHKYKDSREQFEMRTHKRLIDIVNPTPKTVDALMKLDLPSGVNIEIKL
                     "
     gene            23229..23858
                     /gene="rplC"
                     /locus_tag="IV334_000266"
     CDS             23229..23858
                     /gene="rplC"
                     /locus_tag="IV334_000266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HAP8942366.1"
                     /translation="MTKGILGKKVGMTQIFTESGELIPVTVVEATPNVVLQVKTMETD
                     GYEAIQVGYQDMREVLSNKPAKGHVAKANTAPKRFIREFKNVELGEYEVGKEITVDVF
                     QAGDIIDVTGTTKGKGFQGVIKRHGQSRGPMAHGSRYHRRPGSMGPVAPNRVFKNKRL
                     AGRMGGNRVTIQNLEVVRVDAERNVILIKGNVPGAKKSLITIKSAVKAK"
     gene            23886..24509
                     /gene="rplD"
                     /locus_tag="IV334_000267"
     CDS             23886..24509
                     /gene="rplD"
                     /locus_tag="IV334_000267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HAP8942367.1"
                     /translation="MPNVALFKQDGSQNGEITLNEEIFGIEPNESVVYDAIVMQRASL
                     RQGTHAVKNRSAVRGGGRKPWRQKGTGRARQGSIRSPQWRGGGVVFGPTPRSYSYKLP
                     KKVRRLAMKSVLSEKVAENNLVAIEGLNFDAPKTKEFKQVLANLSIDSKVLVVLEKGN
                     DFAALSARNLPNVSVVTSDNVSVLDVVSNTKVLATQTALTQIEEVLA"
     gene            24509..24799
                     /gene="rplW"
                     /locus_tag="IV334_000268"
     CDS             24509..24799
                     /gene="rplW"
                     /locus_tag="IV334_000268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HAP8942368.1"
                     /translation="MNLLDVIKRPVITEKSMLAMDDKKYTFEVDTRANKTLVKQAVEA
                     AFDVKVKNVNIVNVRAKFKRMGKYAGYTKKRRKAIVTLTEDSKEIQLFEAAE"
     gene            24838..25671
                     /gene="rplB"
                     /locus_tag="IV334_000269"
     CDS             24838..25671
                     /gene="rplB"
                     /locus_tag="IV334_000269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010747214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HAP8942369.1"
                     /translation="MAIKKYKPTSNGRRNMTGSDFAEITTSTPEKSLLQPLKNHAGRN
                     NNGRITVRHQGGGHKRQYRVIDFKRNKDNVVATVQTIEYDPNRSANIALVHYEDGVKA
                     YILAPKGLEVGMKLVSGPEADIKVGNALPLENIPVGTVIHNIEMKPGKGGQLIRSAGT
                     SAQVLGKEGKYVLIRLNSGEVRMILAACRATIGSVGNEQHELINIGKAGRSRWMRKRP
                     TVRGSVMNPNDHPHGGGEGKAPIGRKAPVSPWGQPALGYKTRNKKAKSDKLIVRRRKN
                     K"
     gene            25714..25992
                     /gene="rpsS"
                     /locus_tag="IV334_000270"
     CDS             25714..25992
                     /gene="rpsS"
                     /locus_tag="IV334_000270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000533765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HAP8942370.1"
                     /translation="MGRSLKKGPFVDEHLMKKVEAQAGVEKKKVIKTWSRRSTVFPSF
                     VGYTIAVYDGRKHVPVYIQEDMVGHKLGEFAPTRTYRGHAADDKKTKR"
     gene            26015..26362
                     /gene="rplV"
                     /locus_tag="IV334_000271"
     CDS             26015..26362
                     /gene="rplV"
                     /locus_tag="IV334_000271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HAP8942371.1"
                     /translation="MAEQITSAKATAKTVRTSPRKARLVIDLIRGKSVADAISILKFT
                     PNKSAGIIEKVLMSAVANAENNFDLDVENLVVSEAFVNEGPTMKRFRPRAKGSASPIN
                     KRTSHITVVVSEK"
     gene            26376..27032
                     /gene="rpsC"
                     /locus_tag="IV334_000272"
     CDS             26376..27032
                     /gene="rpsC"
                     /locus_tag="IV334_000272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000529931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HAP8942372.1"
                     /translation="MGQKVHPIGMRVGIIRDWDAKWYAEKEYAEFLHEDLRIRKFIST
                     KLADAAVSTVEIERATGRVNISIHTAKPGMVIGKGGSEVESLRKELNKLTGKRIHINI
                     VEIKKPDLDAKLVGEGIARQLENRVAFRRAQKQAIQRSMRAGAQGIKTQVSGRLNGAD
                     IARSEGYSEGTVPLHTLRADIDYAWEEADTTYGKLGVKVWIYRGEILPTKKNTEKGGK
                     "
     gene            27035..27469
                     /gene="rplP"
                     /locus_tag="IV334_000273"
     CDS             27035..27469
                     /gene="rplP"
                     /locus_tag="IV334_000273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HAP8942373.1"
                     /translation="MLVPKRVKHRREFRGKMRGEAKGGKEVAFGEYGLQAVDSHWITN
                     RQIEAARIAMTRYMKRGGKVWIKIFPHKSYTAKAIGVRMGSGKGAPEGWVAPVKRGKI
                     MFEIAGVPEEVAREALRLASHKLPMKTKIVKREEMGGESNEG"
     gene            27459..27647
                     /gene="rpmC"
                     /locus_tag="IV334_000274"
     CDS             27459..27647
                     /gene="rpmC"
                     /locus_tag="IV334_000274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HAP8942374.1"
                     /translation="MKVKEIRELTTAEMLDQEKQLKEELFNLRFQLATGQLENTARIK
                     EVRKSIARIKTVLREQAK"
     gene            27672..27938
                     /gene="rpsQ"
                     /locus_tag="IV334_000275"
     CDS             27672..27938
                     /gene="rpsQ"
                     /locus_tag="IV334_000275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HAP8942375.1"
                     /translation="MTEGRNQRKVYQGRVVSDKMDKTITVAVETKKNHPIYGKRMNYS
                     KKYKVHDENNTAKVGDIVRIMETRPLSATKRFRLLEVVEEAVII"
     gene            27996..28364
                     /gene="rplN"
                     /locus_tag="IV334_000276"
     CDS             27996..28364
                     /gene="rplN"
                     /locus_tag="IV334_000276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002460061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HAP8942376.1"
                     /translation="MIQAESRLKVADNSGAREILTIKVLGGSGRKTANIGDVIVATVK
                     QATPGGVVKKGDVVKAVIVRTKSGARRTDGSYIKFDENAAVIIRDDKSPRGTRIFGPV
                     ARELRENNFMKIVSLAPEVL"
     gene            28401..28709
                     /gene="rplX"
                     /locus_tag="IV334_000277"
     CDS             28401..28709
                     /gene="rplX"
                     /locus_tag="IV334_000277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HAP8942377.1"
                     /translation="MFVKKGDKVKVITGKDKNKEGVVLAAFPKKDKVIVEGVNVVKKH
                     QKPSQAAPQGGIVEMEAPIHVSNVMVVDSTGVAGRVGYKEVDGKKVRVSKKTGEVLDK
                     "
     gene            28737..29276
                     /gene="rplE"
                     /locus_tag="IV334_000278"
     CDS             28737..29276
                     /gene="rplE"
                     /locus_tag="IV334_000278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016185989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HAP8942378.1"
                     /translation="MNRLKEKYLKEVTPSLMEKFNYSSVMQTPKVEKIVINMGVGDAV
                     SNAKNLDKAVEELTLITGQKPMITKAKKSIAGFRLREGMPIGAKVTLRGERMYEFLDK
                     LVSVSLPRVRDFHGVSKKAFDGRGNYTLGIKEQLIFPEVDYDLVDKVRGMDIVIVTTA
                     NSDEESRELLTQLGMPFQK"
     gene            29295..29480
                     /locus_tag="IV334_000279"
     CDS             29295..29480
                     /locus_tag="IV334_000279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011245027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type Z 30S ribosomal protein S14"
                     /protein_id="HAP8942379.1"
                     /translation="MAKKSMIAKNKRPAKFSTQAYTRCERCGRPHSVYRKFHLCRICF
                     RELAYKGQIPGVKKASW"
     gene            29516..29914
                     /gene="rpsH"
                     /locus_tag="IV334_000280"
     CDS             29516..29914
                     /gene="rpsH"
                     /locus_tag="IV334_000280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010742763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HAP8942380.1"
                     /translation="MVMTDPIADFLTRIRNANMVKHESLEVPASKIKRDIAEILKREG
                     FVRDVEYIEDDKQGVIRVFLKFGKNGERVITNLKRISKPGLRAYVKADEVPKVLNGLG
                     IAIISTSEGVVTDKEAREKNIGGEVIAYVW"
     gene            29946..30482
                     /gene="rplF"
                     /locus_tag="IV334_000281"
     CDS             29946..30482
                     /gene="rplF"
                     /locus_tag="IV334_000281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HAP8942381.1"
                     /translation="MSRIGNKVVVIPEGVTVTQDGNNVTVKGPKGELTRTFSADIKMN
                     IEGNEVTFTRPNDSKEMKTIHGTTRANFNNMVVGVSEGFQKALELIGVGYRAQLQGSK
                     LVLNVGYSHPVEITPPAGVTVEVPANTQVIVKGANKEEVGELAANIRGTRPPEPYKGK
                     GIRYVGEYVRRKEGKTGK"
     gene            30643..30999
                     /gene="rplR"
                     /locus_tag="IV334_000282"
     CDS             30643..30999
                     /gene="rplR"
                     /locus_tag="IV334_000282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014125626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HAP8942382.1"
                     /translation="MITKPDKNKTRQKRHRRVRNNISGTAERPRLNVFRSNKNIYAQI
                     IDDVAGVTLASASTLDKEISGGTKTEAAQAVGKLVAERAAEKGIKVVVFDRGGYLYHG
                     RVQALAEAARENGLEF"
     gene            31020..31520
                     /gene="rpsE"
                     /locus_tag="IV334_000283"
     CDS             31020..31520
                     /gene="rpsE"
                     /locus_tag="IV334_000283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HAP8942383.1"
                     /translation="MVYIDPKHLELEDRVVAINRVTKVVKGGRRLRFAALVVVGDKNG
                     HVGFGTGKAQEVPEAIRKAIEDAKKNLVEVPMVGSTIPHEVIGAFSGGRILMKPAVEG
                     SGVAAGGPVRAVLELAGVADITSKSLGSNTPINVVRATVEGLKQLKRAEEVAELRGKS
                     VEELIG"
     gene            31535..31714
                     /gene="rpmD"
                     /locus_tag="IV334_000284"
     CDS             31535..31714
                     /gene="rpmD"
                     /locus_tag="IV334_000284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HAP8942384.1"
                     /translation="MAELKVTLKRSIIGRPQNQRDTVKALGLTKINSSVVKPSNEAIK
                     GMINTVSHLVDVEEI"
     gene            31758..32198
                     /gene="rplO"
                     /locus_tag="IV334_000285"
     CDS             31758..32198
                     /gene="rplO"
                     /locus_tag="IV334_000285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HAP8942385.1"
                     /translation="MKLHELKPAEGSRQVRNRVGRGTSSGNGKTAGRGQKGQKARSGG
                     GVRLGFEGGQTPLFRRLPKRGFTNVNRKDYAVINLDVLNRFEDGAEVTPVALVEAGIV
                     KNEKAGIKVLANGELNKKLTVKAAKFSKAAQEAIEAAGGSIEVI"
     gene            32198..33493
                     /gene="secY"
                     /locus_tag="IV334_000286"
     CDS             32198..33493
                     /gene="secY"
                     /locus_tag="IV334_000286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HAP8942386.1"
                     /translation="MFRLLKDAFKVKDIRSKILFTVFVLFVFRLGAHITVPGVNAASL
                     QNLSSLPFFNMLNLVSGSAMQNFSIFSMGVSPYITASIVIQLLQMDIVPRFVEWSKQG
                     EVGRKKLNQATRYLTLVLAFIQSVGITAGFNQYAQLGFVNNPNVATYVTIGLILTAGT
                     MFVTWLGEQITEKGIGNGVSMIIFAGIISRLPESIREIYEDYFINIDPSDIWKSAIFV
                     AILLVAVLLIVTFVTFFQQAERKIPIQYTKRVAGAPTSSYLPLKVNAAGVIPVIFASS
                     FIATPNAVLQALSGRFGSENWYKVMMEIFNYNTAPGATIYTILIVAFTFFYAFVQVNP
                     EKLAENLQKQGSYIPSVRPGKGTEEYVSRLLMRLSTVGAVFLGLVALLPIIAQMVWDL
                     PQSIGLGGTSLLIVIGVALETAKQLEGLMLKRKYVGFIE"
     gene            33555..34202
                     /locus_tag="IV334_000287"
     CDS             33555..34202
                     /locus_tag="IV334_000287"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HAP8942387.1"
                     /translation="MNLILMGLPGAGKGTQAEKIIDAYGIPHISTGDMFRAAMQNETA
                     LGLEAKSYMDKGALVPDEVTNGIVKERLAEPDTEKGFLLDGFPRTLDQAKALDAMLKD
                     LNKKIDAVIDIHVGEEILVERLAGRFICSNCGATYHKIFNPTKVEDTCDRCGGHEFYQ
                     REDDKPETVKNRLAINIKNSEPILAYYKEQGLLNTIDGAREIDAVFADVQKIIEK"
     gene            34393..34611
                     /gene="infA"
                     /locus_tag="IV334_000288"
     CDS             34393..34611
                     /gene="infA"
                     /locus_tag="IV334_000288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003720929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HAP8942388.1"
                     /translation="MAKEDMIEVEGTVVETLPNAMFKVELENGHQVLATVSGKIRMHY
                     IRILPGDKVTVELSPYDLNRGRITYRFK"
     gene            34644..34760
                     /gene="rpmJ"
                     /locus_tag="IV334_000289"
     CDS             34644..34760
                     /gene="rpmJ"
                     /locus_tag="IV334_000289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HAP8942389.1"
                     /translation="MKVRPSVKPMCEHCKVIRRKGRVMVICPANPKHKQRQG"
     gene            34778..35143
                     /gene="rpsM"
                     /locus_tag="IV334_000290"
     CDS             34778..35143
                     /gene="rpsM"
                     /locus_tag="IV334_000290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008809894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HAP8942390.1"
                     /translation="MARIAGVDIPRDKRVVVSLTYIYGIGNTTAKKILADAGVSEDVR
                     VRELTNEQTDAIRAEVDKLKVEGDLRREVNLNIKRLMEIGSYRGIRHRRGLPVRGQNT
                     KNNARTRKGPARAIAGKKK"
     gene            35171..35560
                     /gene="rpsK"
                     /locus_tag="IV334_000291"
     CDS             35171..35560
                     /gene="rpsK"
                     /locus_tag="IV334_000291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016615824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HAP8942391.1"
                     /translation="MAAKKVNRKRRVKKNIEAGIAHIHSTFNNTIIMITDTHGNALAW
                     SSAGSLGFKGSKKSTPFAAQMAAETATKAAMEHGLKTVEVTVKGPGSGREAAIRSLQA
                     TGLEVTAIRDVTPVPHNGARPPKRRRV"
     gene            35640..36578
                     /locus_tag="IV334_000292"
     CDS             35640..36578
                     /locus_tag="IV334_000292"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010774253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HAP8942392.1"
                     /translation="MIEFEKPRIAKIDEEKDYGKFIVEPLERGYGTTLGNSLRRILLS
                     SLPGAAITNIQIDGVLHEFSTIKGVREDVTQIILNIKGLALKMYGQEEKTLEIDITGP
                     ATVTAGDIIVDSDVEILNKDMYICSVSEGATFHARLTVKPGRGYVQADENKKEDMPIG
                     VLPVDSIYTPVRRVNYQVENTRVGHREDFDKLTMEIWTDGSIEPLEAMSLSAKIMTEH
                     LDIFVNLTDEAKNAEIMVEKEETQKEKMLEMTIEELDLSVRSYNCLKRAGINTVQELT
                     NKSEPEMIKVRNLGRKSLEEVKAKLHDLGLGLRKED"
     gene            36606..36986
                     /gene="rplQ"
                     /locus_tag="IV334_000293"
     CDS             36606..36986
                     /gene="rplQ"
                     /locus_tag="IV334_000293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HAP8942393.1"
                     /translation="MSYRKLGRTSSQRKAMLRDLTTDLLINERIVTTEARAKEVRSTA
                     EKMITLGKRGDLHARRQAAAFVRNEVASVREENEDIVIESALQKLFNDIAPRYAERQG
                     GYTRILKTEPRRGDGAPMVVLELV"
     gene            37258..38097
                     /locus_tag="IV334_000294"
     CDS             37258..38097
                     /locus_tag="IV334_000294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HAP8942394.1"
                     /translation="MEPIIELEKINYKYQPDDLRPALKDVSFTIDKGEWIAIIGHNGS
                     GKSTLAKTINGLLLPESGIVKVGNQILDEENIWTIRQMVGMIFQNPDNQFVGSTVEDD
                     VAFGLENQGIPREEMLVRVKDALEKVRMAEFASREPARLSGGQKQRVAIAGVVALRPD
                     IIILDEATSMLDPEGREEVISTIKKIKEESQLTVISITHDIDEAANANRILVMRQGEL
                     VREGTPKEIFSAGPELIDLGLDLPFPEKLKSALKERGVDVPSEYMTEERMVDWLWTSV
                     LNK"
     gene            38073..38942
                     /locus_tag="IV334_000295"
     CDS             38073..38942
                     /locus_tag="IV334_000295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HAP8942395.1"
                     /translation="MDIRFEQVDFTYQPNTPFEQRALFDINMTIKENSYTALVGHTGS
                     GKSTLLQHLNALVKPTSGTVHIGERDIQPDTDNKNLKPIRKKVGIVFQFPEAQLFEET
                     VAKDIAFGPKNFGVSEEEALVLAKETLEQVGLDESYLERSPFELSGGQMRRVAIAGVL
                     AMRPEVLVLDEPTAGLDPQGRKEMMEMFWRLHKEQQITIVLVTHLMDDVANFADYVYV
                     LEKGRIVNSGEPQEVFQNIEWLKEKQLGVPTATEFAEELMAKGMNFATLPLTAEELAD
                     AIVHYAGGNAHDE"
     gene            38932..39729
                     /locus_tag="IV334_000296"
     CDS             38932..39729
                     /locus_tag="IV334_000296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HAP8942396.1"
                     /translation="MMNKLIFGRYMPGDSLIHRLDPRAKLLASFYFIGIIFLANNWET
                     YLLLAFFTLLSVFLSKVDLKFFIRGIRPLIWLILFTVALQVLFTSGGETYWSWGVFHI
                     TEFGVMNGLFIFCRFVLIIFMSTLLTLTTPPLELSDAIEYLLRPLKAVRFPVHEISLM
                     LSIALRFVPTLMDETEKIMNAQRARGVDFGEGNLVQKMKAVIPLLIPLFVSSFNRAED
                     LATAMEARGYKGGEGRTKYRVLHWHNRDTMVIIAFVLLTVLLIFLRG"
     gene            39932..40678
                     /gene="truA"
                     /locus_tag="IV334_000297"
     CDS             39932..40678
                     /gene="truA"
                     /locus_tag="IV334_000297"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HAP8942397.1"
                     /translation="MVRYKAIIAYDGTNFNGFQKQPNGRTVQEEVEKTLQKMANGKEI
                     TVFGSGRTDAGVHAIGQVIHFDYPEERPLERMRFALDTQSPEDIAVRQVEIVSEDFHA
                     RYLVKEKTYQFRVDIGKPRSPFRRHYASYFPYPLDLEKIQRALPDLLGTHDFTSFCAS
                     GSSIEDKIRTIYEAKMEVNETKDELLFTFRGNGFLYKMIRILVGTLLKIGNGRLPEDS
                     IPDIIAKKDRNAAGPTAHPEGLYLYEVKYE"
     gene            complement(41011..43680)
                     /locus_tag="IV334_000298"
     CDS             complement(41011..43680)
                     /locus_tag="IV334_000298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhO family protein"
                     /protein_id="HAP8942398.1"
                     /translation="MKKNLKSFFRENGRYAAASFLIPFLILALVYLSIGIYPGSSRSI
                     LASDAFSQFSNFHASFRNVLLGKQSIFYTWNASLGLNYLSLISYYLGGIFTPLVFFFP
                     NQMMPDALYVLTLLKVGCAGLAFWFLARTFKIPRWGHVALSVSYALISFITAHSEIIM
                     WLDAFVYLPLVILGIHRVMDLKRPKLLFISYLLLFLSSFYMGFMIGVFSFLYFIARLL
                     TNWSEYKKTIVPYGITSLLAGGASMIIVLPAVLDLRSNGEALSQITTLKTEATSYLDL
                     IMKNMIGVYDTTKYGSIPFVYAGLLPLVLCLFYFASRKVPLKNKFLYGSLFAILIASF
                     YLVPLNLFWHGMHAPNMFLFRYAYLFSFLVLLLAGKGWEKIEQDDGGLLLGIILVVAA
                     LFAVAWGIKPEGSYTYVTMTSFVLTVVFLGIYAFAVGFYQHKKLSFNHLSILLLLIMS
                     AEALVNTNSMIHGILDDWNYASRSLYTDPYPDLKALVNETKKDSDSFYRLENLDPVSA
                     NDSINYGYSGISLFSSIRNRNSSSYLDKLGFRSRGTNLNIRYNNNTLLMDGFTGIKYN
                     ISKNESTFSKYGFRQAGQSGDYTLYQNVNALPLAFTAPLSVNDVEQPVTDNLTSQTNL
                     INTLSGLNEQYFTFYTPTLKNQLNTTITKTTTGVTYSETADNQPKELTWEVHVPANTQ
                     AYLSLFPTNFAQLESSSATITVNGTSRKTQINISGQYYDLGYYPQETTVTFTASFYGT
                     KEISFMEPKVVGLDVVAYQAAMNQIKEKGVEMEATGRKASGTVNTAEDSMLVTTIPYD
                     KGWKAKIDGKPVKVSNFKDAFLMIKVPAGKHTVTFSYLPEGFTIGAILFVLCIALFIL
                     NVWYLDRNRPFYGTIDPAIEEQPRNTHRRRRRK"
     gene            complement(43918..44106)
                     /locus_tag="IV334_000299"
     CDS             complement(43918..44106)
                     /locus_tag="IV334_000299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HAP8942399.1"
                     /translation="MAKVCYFTGRKTKSGNNRSHAMNSTKRTVKPNLQKVRVMVDGKP
                     KKVWVSTRALKSGKVERV"
     gene            44376..44738
                     /locus_tag="IV334_000300"
     CDS             44376..44738
                     /locus_tag="IV334_000300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HAP8942400.1"
                     /translation="MAVKIQTSAGTIEITNDVIATVVGGAATDVFGVVGMASKNQIKD
                     NITEILRKENYSRGVVVRQEDNGIAVDVYTIISYGTKISEVSRNVQEKVKYNLEALLG
                     VTANSVNVFVQGVRVLPD"
     gene            44790..46472
                     /locus_tag="IV334_000301"
     CDS             44790..46472
                     /locus_tag="IV334_000301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DAK2 domain-containing protein"
                     /protein_id="HAP8942401.1"
                     /translation="MSVTEISASQFQEMVQAGANRLQVNAEYVNSLNVFPVPDGDTGT
                     NMNLSMTSGAKAVVDSTSEKVGELAAMLSKGLLMGARGNSGVILSQLFRGFSKQIPDV
                     VSLNASDLAAAFTHGVETAYKAVMKPVEGTILTVAREAAKAGEKKAKSTDDVVEVMTA
                     VVKGAKRALAKTPDLLPVLKEVGVVDSGGQGLLFVYEGFLSALNGEFQADETYEPSPA
                     EMDDMVNAEHHRSVQGQMATEDIHFGYCTEIMVKIGEGPTVDSTFDYEEFRNYLDGIG
                     DSLLVVNDDEIIKVHVHTENPGEVLNYGQKFGSLVKIKVDNMRLQHETILEHDEEVNE
                     FAKKEIPRIPYGIVAIAAGKGVQELFESLGANYVISGGQTMNPSTEDIVKAIQEVNAD
                     KVIVLPNNKNIFMAADQAAEVADIPVAVVPSRTISQGLTAMLSFNDQASLEENKETMT
                     EMLSSVVSGQVTHAIRDTSIDGVEIKEGDFLGMIDGKILISNPDITATALSTLEQMIN
                     EDTEIVTILAGDEGTVEQANEFAEKLTEKHPDLEIEIHQGDQPVYPYLFSAE"
     gene            46589..48625
                     /gene="recG"
                     /locus_tag="IV334_000302"
     CDS             46589..48625
                     /gene="recG"
                     /locus_tag="IV334_000302"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HAP8942402.1"
                     /translation="MRSLDDPITMLAGVGEKRAASLASLGIQTIEDLLTYYPFRYEDI
                     QERNLNEIQDQEKVTLKGIVVSPPVMNRFGYKKSRLQFRMMQEHDVFNVSFFNQPYLK
                     EKVILSEEIAVYGKWDAKRKALNGMKILGSQTMDDFSPIYHVNKSIRQTTLVDLIKKG
                     FQEYGTLIEENLPNNLVEKYRLLDRASAVRSMHFPKDHEENHQAKRRVVFEEFFLFQM
                     RLQGLKKAEKAETNGLEILYDVQKLKQFTQKLPFELTNAQKRVTNEICRDLRSPQHMQ
                     RLLQGDVGSGKTVVAAIALYATVTAGFQGALMVPTEILAQQHMESLQQLFDPNEVTIA
                     LITGSTKAKERRELLEKLEQGEINVVIGTHALIQDGVEFQNLGLVITDEQHRFGVNQR
                     KVLREKGWRPDVLFMTATPIPRTLAITAYGEMDVSIIDELPAGRIPIETRWVRTPQLD
                     SVLDWTKKELARGHQMYVICPLIEESEALDVKNAVEIYEKLRELFAPKYEVGLLHGKM
                     KNQEKEVIMEAFKDNQMQILVSTTVIEVGVNVPNATVMLIMDADRFGLAQLHQLRGRV
                     GRGSDASYCILVANPKNELGVERMKIMTETTNGFVLSEKDLELRGPGEVFGFRQSGLP
                     QFAIADLVTDGNVLEVAREEAAALWQIKDWQMLPEYQPLAQSLQIENQDNRYFD"
     gene            48674..49675
                     /gene="plsX"
                     /locus_tag="IV334_000303"
     CDS             48674..49675
                     /gene="plsX"
                     /locus_tag="IV334_000303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HAP8942403.1"
                     /translation="MKIAVDAMGGDHAPQAIVEGVALAQQDFPEVEFLLYGKEAEIKK
                     YLTNEKNITIIHTDEKINSDDEPVKAIRRKKTASMVLAAQAVKNGEADAVFSAGNTGA
                     LLAAGLFIVGRIKNIERPGLMSTLPVVGKEGAGFDMLDLGANAENKPEHLLQYGILGS
                     FYARKVRGIGRPRVALLNNGTEATKGSEVTKKAYELLQNETSLNFIGNVEARELLNGV
                     ADVVVTDGFTGNAVLKSIEGTAMNMMNLLKSAILNEGIKGKMGAMLLKDGLRSLKAEM
                     DYSKHGGAVLFGLKAPVIKTHGATGPDAVRYTIRQIHTMLETDVVGQLVEQFEKSEE"
     gene            49797..50039
                     /gene="acpP"
                     /locus_tag="IV334_000304"
     CDS             49797..50039
                     /gene="acpP"
                     /locus_tag="IV334_000304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HAP8942404.1"
                     /translation="MTREEVFNKVAKIISNHFEIDTDKVTDELNIKDDLKADSISIME
                     FVLELEDEFGTEISDEDAEQIETVGGAVDYISSHLK"
     gene            50307..51311
                     /locus_tag="IV334_000305"
     CDS             50307..51311
                     /locus_tag="IV334_000305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP8942405.1"
                     /translation="MSDNQLLEVQNLHTGFRLKDDYYDAVDDVSFTLDKNEILAIVGE
                     SGCGKSTLATTIMGLLDPNNTKITGEILYNDLNLIDLNETLYNKIRGNDIGMIFQDPL
                     SALNPLMRIEDQIKEGLSYHTKMNAEQRQARALELLEQVGIPNPARVGRQYPHELSGG
                     MRQRVIIAIAIACKPPIVIADEPTTALDVTIQAQILDLLKDLQEETKTGIILITHDLG
                     VVAEMADRVAVMYGGQFVEVAGVKELFDHPKHPYTRSLLNSIPQEGNHDAELHVIEGV
                     VPSLKNMPRKGCRFAPRIPWIPASDHEEEPILHEVAPNHLVRCSCYKHFHFRDEKGEV
                     "
     gene            51312..52256
                     /locus_tag="IV334_000306"
     CDS             51312..52256
                     /locus_tag="IV334_000306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP8942406.1"
                     /translation="MTELITIKDLKVHYPIRSGFFNRVTDHVYAVDGVDFIIEKGKTY
                     GLVGESGSGKSTTGKAVVGLEKVTAGQIMYEGKDVTKRSNRKKMGYNKDVQMIFQDSM
                     SSLNPKKRVLDIIAEPIRNFERLSDQEEKKKVKGLLDIVGMPEDALYKYPHEFSGGQR
                     QRLGVARAVATNPKLIVADEPVSALDLSVQAQVLNFMKRIQQEYGLSYLFISHDLGVV
                     KHMCDNIAIMYKGRFVEIGTREDIYNDPRHIYTKRLLSAIPQIDVENRESHKENRRRV
                     EQEYIQNQKEYYDATGRVYDLRTITTTHKVALKDGGAS"
     gene            52256..53218
                     /locus_tag="IV334_000307"
     CDS             52256..53218
                     /locus_tag="IV334_000307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP8942407.1"
                     /translation="MWKTILRRVLLMIPQVIILSVLIFLLAQAMPGDPFTGLINPNQD
                     PAVIEQMRQAAGLNDPWYVQYFRWIGNALQGDFGQSFLYKRSVADLIGERIINTLYLS
                     ILTVVLTYLIAVPLGMLAGRYQNSILDKIVVIYNFFSFAVPLFIFGLIMLFVFGYRLG
                     WFPTSGSQTLGLQGGFITQWIDRLKYILLPSITQAFLATAVTIQYLRSEVIDSKSLDF
                     VRTARSKGVPTNKVFSRHIFRNAALPMASQMGYEITSLIAGSVVIEKIFGYPGVGKLF
                     IDSIGQRDYTVITSLVLILGIATLVGTLLSDIIMSIVDPRIRIQ"
     gene            53245..54159
                     /locus_tag="IV334_000308"
     CDS             53245..54159
                     /locus_tag="IV334_000308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP8942408.1"
                     /translation="MSEKNNTAVQESIPPMGIRMIAREFKKDKIAIFSLGLLVVLLLV
                     IFIGAMLTDQDKVMYVSIFDKYAAPGTVSTQGQKFILGADEGGRDVLGQLIIGARNSV
                     TIGFAITILTSIIGVGLGIISGYYGGIIDNILMRIVDFIMILPIMLIIIVFVSIISRY
                     NVWSFIFIMSAFYWVAKARLFRSKTLSEARRDYVSASKTMGTNDFKIMFREIMPNLSS
                     LIITNLTMNFAANIGIETTLTFLGFGLPANVPSLGTLIGYASNGEVLVNRQWIWLPAS
                     ILILVLMLSINYVGQAFKRSADARQRLG"
     gene            54185..55966
                     /locus_tag="IV334_000309"
     CDS             54185..55966
                     /locus_tag="IV334_000309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HAP8942409.1"
                     /translation="MKKTIFGFAALMSAVVLAACGGGGTDTSGNNSGGNGNNKEELTF
                     PIATKNNDDAIEDGELEAAVATDSQFKGLFQWEFYQDSYDAAFMAPSHEPLFANDDDF
                     RITDDGAATLDLDEDTKKATIKVKDNVKWSDGEDVTADDVIFPYEIIGNKDYTGIRYD
                     DTFRNIVGMEEYHDGSADTISGIKKIDDKTVEIEYKEVNPSMLQSGGGIWSYAAPKHT
                     LEGVAIKDMESSDQVRKNPVTFGPYYMSNIVAGESVEYLPNEHYWNGTPKLKKITMKS
                     VPMASATEALNSKLYDMIFQMPTDTYDTYKEIAGYTNLGREQTSYTYLGFKLGKWNAD
                     TKSVEYNPDSKMADKSLRQAMGYALDNNAVGERFYAGLRSNATSLIPPVFQSYHDDEM
                     KGFTQDLDKAKKLLDDAGYKDVDDDGLREDKDGNKLTINFASMSGGETAQPLADYYIQ
                     QWKEIGLDVQYTTGRLIEFNSFYDKLENDDPEIDVYQAAWNTGTDPNPSGLWGPNAAF
                     NYTRYESEENTKLINDISSSKAFDEEYQKEAYQKWQQYAFEEAFAIPTLFRNEVLPVN
                     DRVKDWDWSYDVPNPWANVSVTADSRS"
     gene            56314..57000
                     /locus_tag="IV334_000310"
     CDS             56314..57000
                     /locus_tag="IV334_000310"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HAP8942410.1"
                     /translation="MDNQLTKELKENYNIVFHDLNLLEQAFTHSSYVNEHRNLQLSDN
                     ERLEFLGDAVLELMVSQYLFRLYPHLPEGKLTKTRAAIVREDSLSKFAKECHFDQYVL
                     LGKGEENSGGRTRPALLCDLFEAFLGALYLDQGFDTTHAFIEKVIFPKVKAGAFSHEM
                     DHKTKLQEVLQKSGDVSIEYRLINEEGPAHERVFWIEVYVDDQLIGTGQGKSKKLAEQ
                     AAAENALAAL"
     gene            57020..60601
                     /gene="smc"
                     /locus_tag="IV334_000311"
     CDS             57020..60601
                     /gene="smc"
                     /locus_tag="IV334_000311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HAP8942411.1"
                     /translation="MYLKRIEIAGFKSFADKTVIDFENSVTAVVGPNGSGKSNITEAI
                     RWVLGEQSAKSLRGGKMPDIIFAGSDTRKQLNIAEVTVILDNSDHYLPLEYNEISVTR
                     RYRRTGESEFFINKQSCRLKDIQELFLDSGLGKESFSIISQGKVEAIFSSKPEDRRGI
                     FEEAAGVLKYKQRKKKAEQKLFETEDNLSRVQDIIHELEEQLTPLAAQSEAAKEFLRL
                     KETLTQTDVSLMVAEIKTAKKDWDNKQAQLAKFNLELGKLSESIQEQESILAKQRKEN
                     AQADRLIEKNQQVLLDLSEKLKQTEGQKDVLQERTKHTQKSSQEYQTSLAEAQKKVKH
                     FEKLQESLMKAAAEKETEIQKAEANLIKTQQELEKYQKSTKELLAELRDQYVDLMQEQ
                     AAVGNELKYLERQYIQETAKSKQTLAKQSEVEASVDRLILQKEELTQKQAQLKSSLTE
                     TKEKLEMIQQNGKKFQEALAKEQPKMYQLMNQVQQLRARKKSLQEIQENYFGFYQGVR
                     LVLQHKQQLSGIVGAVAELIDVPADFTLAIETALGGAAQHVIVENEKDARQAITYLKQ
                     QRGGRATFLPLTTIKPRQLPAHILTQAAAVEGFIGIASEQVSYPDQIQTVVQNLLGTI
                     LLAKDLTSANAIAQTIRYQYRVVSLEGDVMNAGGSMTGGANKRGNQGSLFVQNQELKQ
                     LTSEFEEADKQLQAQEKKVQELQQETARLAEEQEVLRTRGEQLRFEEQEATNQLQNII
                     NELERFEKEKQISTFETRELQQFIEDYEKQTNELKDKQTDLESQRQKIDEEIKSLSQE
                     SDQMEARRAQVQSQKAQEQADLAVLKEQFNHLQIQLRGARVQKAEATERQEAIEKQLA
                     TLTADFSDHEVTEESLEKQINELSAQRETLKAELAKAKEQRDRRQKEIDKLEAVLAER
                     NREQKARLSEQSKLEVQKDRAEMILDNHLTYLQSEYQLTFEKASQDYQETTDIEDSRT
                     KVSSLKEQIEKLGPVNLNSIEQYEQVSERHTFLATQRDDLLAAKNQLFETMDEMDDEV
                     RTRFKEVFEAIRQEFKIVFPNMFGGGKAELVLTEPTDLLKTGIEIEVQPPGKKLQSLS
                     LLSGGERALTAIALLFSIIRVCPVPFCILDEVEAALDEANVKRFGRYLSDFQDDTQFI
                     VVTHRKGTMVAADVLYGVTMQESGVSKIVSVRMEDINEEAKINETKV"
     gene            60610..61419
                     /locus_tag="IV334_000312"
     CDS             60610..61419
                     /locus_tag="IV334_000312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAP8942412.1"
                     /translation="MIKLVAIDLDGTLLNEQKLISDENKQALAQAKQQGVKIVLCTGR
                     PLAAMAHYLQELGLVDEGDYSITFNGGLVQKNDTGEIMEKKVMEVSDIQRLYKLAQQL
                     DLPLDVLSDNVVLQLPSAPRKQSLYNVLNKLLQFQPADISDITDEFVLNKAVIAYDQE
                     ELDPKIKEIPAEFHEMYEIIKTRSMLLEFMPKGVTKAYGISLLAKDLGLKQEEIMAIG
                     DEENDLPMIQYAGLGVAMENAVPFVKEAADFVTASNEENGVAKVIQKFVLE"
     gene            61430..62428
                     /gene="ftsY"
                     /locus_tag="IV334_000313"
     CDS             61430..62428
                     /gene="ftsY"
                     /locus_tag="IV334_000313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HAP8942413.1"
                     /translation="MGFFDKIKRAFTGEEKTEEVEVQEKYDKGLEKTRKTFGERMNEL
                     FANFRTVDEDFFEELEETLIGADVGFETAIRITEALRQEVKLRNAKKSAEVQNAIIEK
                     LVDLYEAEGINEVNELNIQEDGLTVILFVGVNGVGKTTSIGKLAHQFKNEGKKVLMAA
                     ADTFRAGAIDQLVVWGERAGVEVVRGNAGGDPAAVVFDALERAKTEQADVLLVDTAGR
                     LQNKVNLMNELEKIKRVIQRELPEAPQETLLVLDATTGQNAMVQAKQFKETTDVTGLV
                     LTKLDGTAKGGIVLAIRNELHLPVKLVGLGEGIDDLEPFDPNDFVVGLFKGLLKEE"
     gene            62615..64882
                     /gene="gshAB"
                     /locus_tag="IV334_000314"
     CDS             62615..64882
                     /gene="gshAB"
                     /locus_tag="IV334_000314"
                     /EC_number="6.3.2.2"
                     /EC_number="6.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutamate--cysteine ligase
                     GshA/glutathione synthetase GshB"
                     /protein_id="HAP8942414.1"
                     /translation="MMNFKQLLLHVNARPFIDQARFGIEREGQRVDLAGNLAKTDHPA
                     IFGDRSYHPYIQTDFSETQTEMITPVTDSIPELFQYLAAVYDVTARSIPKEEMIWPLS
                     MPPALPEKDEEIIIAKLKNFEDVLYRRYLAKEYGKRKQMVSGIHFNFEFGDELLRTLF
                     SHQEEFQDFSEFKTELYLKTARNFMRYRWMITYLFGASPMSEKNYFLDESHPQEPVRS
                     IRNSALGYTNHPNVKVSYASMKQYLADIERMIEEGKLSEEKEFYTPLRFRGGKKVADL
                     ATTGVRYIELRNIDLNPYARLGINPEQVRFLQLFLMYMLWTEEKEDCDQWVAEGTTRN
                     NKVALEQPSDQTEFHQEGREILEGMKQMLVELDWLDSLYLVEEALTQMDHPEQTLAAK
                     LYQEAQLSSQQEVAVALGHQYYKESHERPYQLAGFREMELSTQIFMFDAIQKGVQVKV
                     LDESDQFLRLQFQDHVEYVKNANMTSKDSYIVPLIMENKTVTKKVLKEAGFRVPGGAE
                     FSSMEEAVKAYPRFAEQAFVIKPKSTNYGLGITIFKEGASLEDYQAGLAIAFREDSSV
                     LVEEFMPGTEYRFFVIDGEVQAIMLRVPANVIGDSIRTVKELVEEKNSDPLRGTNHRA
                     PLELIQLGELEQLMLKEQGLTIESVPQANQIVYLRENSNISTGGDSIDMTDEFSEAYK
                     KIAVSAVEALGAKISGIDLIIPDKEIDPTTDKKAYGIIEANFNPAMHMHVYPFAGKGR
                     RLTMNVLKLLYPEVF"
     gene            complement(64971..65423)
                     /locus_tag="IV334_000315"
     CDS             complement(64971..65423)
                     /locus_tag="IV334_000315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YueI family protein"
                     /protein_id="HAP8942415.1"
                     /translation="MAQDDVKSHLDKGLYGTPLVNPEEQHKYLGTFRERCYLSMTVAE
                     MIQPKNKENFLKELKKHPDASILLNGALPLSVQNQYIQLATKNNSRFTVVNDYVTANP
                     EEFGLLLTAKEAVNEPVIDIEKKYPEENSSPAEKEKPKKKKSFWDRLF"
     gene            65573..66523
                     /locus_tag="IV334_000316"
     CDS             65573..66523
                     /locus_tag="IV334_000316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HAP8942416.1"
                     /translation="MRYGRLVALLVILSSISLFIGVSNVQLSELLQLDGQSLQILLVT
                     RLPRTICLILVGATSSICGLIMQHLTQNKFVSPTTAGTMDSARLGILVAMIFLPGASL
                     IVRSLTAFCFAFAGTLLFLSLTRLFLQKNQLMLPLIGVMLGNIIGSVATFFAYQFQLV
                     QNMTSWLQGNFATVIRGNYELLYAVVPLLLLVWLFAYRFTIIGLGESFAVNLGIDYQK
                     MQFLGIGLVSLASAVMLIMVGSIPFIGVIIPNLVSRIYGDQVHKTIGVTALAGSLFLV
                     FCDLVARVLIFPYEIPVSVIVGIIGGAIFLYLLIRGRRYA"
     gene            66516..67475
                     /locus_tag="IV334_000317"
     CDS             66516..67475
                     /locus_tag="IV334_000317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HAP8942417.1"
                     /translation="MLKKKPSIFIGMLVLVLFVTGMYLTIHTYGNWHFACALRSKKIF
                     AFILVSLAVSSATISFQTITHNQFLTPSVLGLESLYVLVQTVLFFFIGGVTMLQQTGT
                     VFFLLNVGLMVGLSLLLFFFLLKKMRANLFLLLMSGMILGTLFGSVSTFLQVIMDPNE
                     YDLLQGRLFASFGNIQSQYLLPASLIIIGCWYLLFRYRHVLDVFHLGNEQAVNLGIDT
                     DRFEFLILCCVSLLTGTSTALVGPVTFLGFIVANISYRVFSTYRHGQLFIGSFLVAML
                     FLTGGQLAVEQIFGWNTTVSVVVEFVGGIYFVTKLIRERKSIL"
     gene            67472..68227
                     /locus_tag="IV334_000318"
     CDS             67472..68227
                     /locus_tag="IV334_000318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAP8942418.1"
                     /translation="MIDLQKVNKLYHGKSVVKDVQLTIPEKKLTAFIGPNGAGKSTVL
                     SMISRLIPKDTGEIYLDHNEVKAWKSNELAKKLSILRQSNEINVQITVKELVEFGRFP
                     YTKGKLTTKDEEIVQESLEHLGLEEIADQSIDTLSGGQLQRAYIAMVLAQDTDYILLD
                     EPLNNLDMNFAVQIMQILKNLVQEIGKTIVIVLHDINFAASYADEIVAMKDGQLFTQG
                     TTQEMIKKDVLDELYEMDIRICEIEGRRFCLYF"
     gene            68250..69203
                     /locus_tag="IV334_000319"
     CDS             68250..69203
                     /locus_tag="IV334_000319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore ABC transporter substrate-binding
                     protein"
                     /protein_id="HAP8942419.1"
                     /translation="MKKLVVGILILASFGLAACGNSNNTSQADTKKSSTQTETTLTIT
                     DSNGDQIEVPNNPEKVVVFDNGSLDTMDALGVGDKVVGAATSSLPEYLSSYKKVESAG
                     GIKEPDLEKINQLQPDLIIISGRQRDFQKDLSQIAPTIFLSLDAKNPWESFQQNVTAL
                     GEIFGKQEEAKTQLEELSSAIDQTKKKAEATDKKALVTLVNEGQLSAYGSGSRFGFIH
                     DLFGFEQADDQIEASTHGQSVSYEYVLEKNPDILFVVDRTKAIGGDDSKDDISANELV
                     AQTNAGKNQQIISLEPDVWYLSGGGLESMKLMIEDVNQAFK"
     gene            complement(69847..70035)
                     /locus_tag="IV334_000320"
     CDS             complement(69847..70035)
                     /locus_tag="IV334_000320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8942420.1"
                     /translation="MDIYKKLASKKTNSFGLILSEYKIKAEESYFYQLLKRWSKLPAD
                     FYRKMQIINYQPLAETHA"
BASE COUNT        22740 a        12775 c        15205 g        19380 t
ORIGIN      
        1 ataagaggat ttctagctct cctacaacta ttcccactcc actgttgctg gtggtttaga
       61 agtgatgtca tacacgatac ggttgacgtg ggctacttcg tttacgatac gtacagagat
      121 tttttgcaag acttcccatg ggatacgggc aaagtcagcg gtcattccgt cgattgaagt
      181 tacagcacgg ataccgactg tgtagtcgta agtacgtccg tcacccataa caccgacaga
      241 gcggatccct ggaagaacag tgaaatattg ccagatatca cggtcaagtc cagcagctgc
      301 gatttcttca cgcaagatag catcagattc gcgtacgatt tctagcttgt cttcagtgat
      361 ttcgccaaga acacgaatac caagaccagg accagggaat ggctgacgcc aaacgattga
      421 atcaggcatg cctaactgag tccctaattc acggacttcg tctttgaata atgtgtttaa
      481 tggttcgatc aattcaaatt gcatatcttc tggcagtcca cctacattat ggtgagattt
      541 gatcgtctgt gctgtttctg taccagattc gataacgtca gtatacaacg ttccttgtgc
      601 taggaaagag acgccttctt caccagcaag ttttgttgct tcgtcatcaa aaacatatac
      661 gaattcattc ccaatgattt tacgtttttg ttctgggtca gatacacctt tcaacttgtc
      721 taagaagcga tctcttgcat cgactttgat gatgttcaag ccgaattttc cgcctagact
      781 ttccattact tgttccgctt cgcctttgcg tagcagaccg tggtcaacaa agatagatgt
      841 caattggtca ccgatcgctt tttgtaaaag aaccccaacg acactagaat caacgccacc
      901 tgaaagacca agcaagactt ttttgtcacc aacttgttca cggatttttt ctacttgcat
      961 atcgataaag ttatccattg tccagttacc ttcacagtga caaacatcaa aggcaaagtg
     1021 gcgcagcaat tcgattccgt attcggaatg acgtacttct gcatggaatt ggataccata
     1081 aaaattacgt gttgtgtctt ggatagaagc aatcggacaa tctttgcttg ttccgactgt
     1141 ttcaaagcct tcaggaactt tagttaccaa gtcaccatgg ctcatccata ctgtttgttg
     1201 ttttggtgtt tctttaaaca agacagcttg atcgcttgtt acttctaaca aggctttccc
     1261 atattcgcgg tttttagcag gctctacgga accgccaagt ttgtacgtga tcaattgcat
     1321 accatagcaa atccctaata caggaatacc taaattgaag atctcaggat cgatcccaaa
     1381 tgcattctca tcatatacac tgtttggtcc gccagaaaga atgattccct ttggattcat
     1441 cgcttttact tcttctgcag taatgcggtg gctcaataat tcggaaaaga ctccaagatc
     1501 acgaatacgt cttgtgatca attggttata ctgactaccg tagtctaata cgataatctt
     1561 ttcaacgttc gtcatatcgg caacgtttgt caaatttatt acccctatcc ctttaaattt
     1621 tatctcaaaa tagtcaaact attttaaaat gctaatattt tcgcatgaaa atcgaatcga
     1681 tgatatgatt ttcagtttta tggagaatta tatcggctct tcctcgtgta ggcaagatat
     1741 attcctgtaa atttttcaaa ttcacatttt tccatacatc ttttgccatt ttaaaggctt
     1801 cttcacgatc tccgatagca aaacgataat aataattctc tggattttgg aaagccgtat
     1861 ccagtaacgc accaaaacga tccaaatacc atttttcgat cagcacggga tcggcatcga
     1921 catagacaga aaaatcaaaa aagtcgctga cgtagatttg ctgattcgca ggtaattgaa
     1981 gcgtattgat cccctcaacg atcaagatat caggctgctg gatcaattcg tacttgtctg
     2041 gaatgatatc atacacatca tgtgagtaaa caggtacttt tatctcttcc tgaccagatt
     2101 ttacacggtt caagaaatcg atcagcatct ccatatcata actttcagga aatcctttac
     2161 ggtccatgat tccacgctct tttaatatac gatttggata aagaaatccg tcagtcgtga
     2221 tcagctgaac gtttcttcgt ttgaaggtac gagacaaaat cgtctgcagc agtctggctg
     2281 ttgtactttt tccaacggct acacttccgg cgatcccgat aataaaaggc ggtacttcta
     2341 cgtatttatg aagaaataaa cctttactca agaacaaaga ttcaaattct ttcatataaa
     2401 gataaatcag atgagcaaga ggtacataga tatctcgaac atcctgcatg gaaatgcggt
     2461 cattcaaact tttgatatta tctagttcat cttgtgtcaa aggtgcttgc ccattacgat
     2521 aaaattctcg ccattcttct cttgccacac ggtagtagtt cattggatca ttcatattag
     2581 cctcctgcgt tctcccatca atggaactat tttaacacaa aataaacgca aaccccacca
     2641 cttaagagag attgccgatc tttttgtata atctttataa tagaaagaaa cggagtctta
     2701 ttcttgactt gcgcacggat acataaaagg atagactgtt gtcatgaaag aaatgagaag
     2761 gatggaagac atgacaaatc attactattc tgaacgccca gatacggcgc acgattttga
     2821 acaatgggct tttgagttaa aaggcagaac ctttcaattt gtaacagatt ctggtgtatt
     2881 ttcacgtgaa acagtggatt atggttcacg tgtcctgatt gatgctttcg attgggaaga
     2941 gttacctgat gaaggaaaga tcttagatgt gggatgcggg tatggaccga ttgggttagc
     3001 tttggcattt gcgacacagc ggtttgttga aatggtcgac attaatgaac gagcggtaag
     3061 tctagcgcaa ggaaacgcaa agcgtaacca aatcgaacat gttgatatcc atcaatcgaa
     3121 tatctatgaa gcagttcatg aagaaaccta tgcagctatc gtaagcaatc cgccgatccg
     3181 tgctggaaaa aaagttgtac atgaaatttt gacaggcgca tatcctcggt tgaagaaggg
     3241 cggtacactg acaattgtga tccagaagaa acaaggagcg ccgagtgcgc aaaagaaaat
     3301 ggaagaaaca tttggtaatg cagaaatcgt cacaaaagat aaaggatatt atatcatcaa
     3361 aagtgtgaaa gaataactag ctgaaaaata gtggagcgac gaaagagtgg aactgggcgt
     3421 gcatgtacgg gtacgtatca gttctttttt tgttgagaag tttttaaaaa caaagaacta
     3481 ttttttcttc aaaaagaaat agtaggaaca gagtcgttgt tttttacgat aaaataaata
     3541 agatacagaa cttgtataga aggctgccaa gtcggtagat aaaggaggtt tttatcaaaa
     3601 aacatacttt ccttttttct ctattctttt cttgggggtt ttggtacact gatagggaat
     3661 ttagatagaa gggagccaat aggatgaagc tgaccatcag agatatcgca gaaatggcag
     3721 gcgtatcagt aacaactgtc tcacagattt tgaataacaa aggaagcaga tttagtgata
     3781 agacaagaaa aaaagtgttg tcgatcgtga atgaacatca ttataagccc gattatttcg
     3841 cttcaaatgt gatcaatcga cattcaaaga ccatcggaat gatcgtccct gatgtcactg
     3901 atttcttctt ctccaaactg atcgaaggtg tagagagcta tttgaatcct ttgggctatg
     3961 tgatcatgtt atgcaattct cgtcatagcc aagaaaatga gatcaagtat ttacaagagc
     4021 tttcccaccg ctcagtggac ggaatcttgc tggccacacc aaatatttta cctgagaaat
     4081 atgcattaga cagcggtttt tatcaaaaaa tgccaatcat tttaatcgat cgcggattga
     4141 atcgtcggga taacggacgg cttatcgtaa aggaatatga gggcgcctat caagctgttt
     4201 cgctgtttat tgaaaatggc catacgaaga tcggcatgct gaaggaaacc actggatact
     4261 atcaattaga agaacgtttc aacggatatc gtcatgcgct gaaagatcat ggcctgcctt
     4321 ttaatgggaa atttgttgaa catggagagt tgactgtcca aggtggatat actgcatcaa
     4381 aaaaattact caaacataaa gatatcacgg ctattttttg cgggaatgat gccatggcga
     4441 tcggatgcta tcaggcgatc gatgaattgg gcaaaaaaat cccagaagat atctcagtca
     4501 taggattcga tggattaaag ctgtcagaat acatgattcc gcaattgacg acagtgcagc
     4561 agccaagttt tgatattgga ttttatgctg cacgtttcct gatcgatacg atcgaatttc
     4621 ctcaaagaaa agttccgaac aaagtgttcg aaacaaaatt aattattcgt gagagcgtaa
     4681 aagtattgac aaagggctaa ctcggcggta tattgcttat gtaagcattt acaaaggcta
     4741 gttagtgctt tttttatttt tagtaaacca gtttactaaa gtggtttacc tttcctaaaa
     4801 ataatgtata ataccgaatg aagacaagga ggatcaggca atgcgaatgg tggatctgat
     4861 tgaaaaaaag agggatggaa acgagctgtc gaaagaggaa atcgagtaca tcgtcacgaa
     4921 ctatacgaat ggaaagattc cagattatca agtcagtgca ttattgatgg caatttttta
     4981 tcaagacatg acaaatgaag aaatcacgaa cttaacattg gcgattgcta attctggtga
     5041 tgtcatcgat ttgagttctc ttgaaggaat aaaggtagat aagcattcga caggtggcgt
     5101 tggtgacacg accacattga ttttagcccc cctagtagct agtgtcggag tgacagtggc
     5161 taaaatgtct ggacgcggtc taggttacac aggtggtaca ctagacaagc tagaagcaat
     5221 tcccggattt cagatcgagc tttcggatga agaatttgta cgcattgtta atgaaagtaa
     5281 agtagcggta attggtcaat caggaaactt ggcaccggcg gacaaaaaat tgtatgcctt
     5341 gcgtgacgtc acagcgaccg ttgattctct tccgctgatt gccagttcga ttatgagtaa
     5401 aaaaatcgcg gcaggagcag atgcaatcgt tttggatgtg accactggag acggtgcttt
     5461 catgaagaac atcgaagatg caagacgtct ggcaaagacc atgacgagca ttgggaaact
     5521 ggctaaccga gaaacagtgg cggttatctc agatatgtca gaacctcttg gcgaagctat
     5581 cggaaatagt ctagaagttg tcgaggcaat cgaaacatta caaggaaatg gtccagaaga
     5641 tctagtagaa atgtgctacg ccttaggaag ccaaatggtt gttcttgctg gcaaagctaa
     5701 gacaaccgat gaagcaagaa cgttgcttca agaagctctg gaatcaggta aagcgttagc
     5761 aaaattcaaa gaaatgatcc aaaatcaagg gggagaccca gcgatcgtgg aacatccaga
     5821 acgaattttg actgcacgct acacaatgga gcttcctgct aaacaaagcg gagttgtgtc
     5881 aaaaatcgtg gcaaatgaat taggaattgc tgcaatgatg ctaggtgctg ggcgtaaaac
     5941 caaagaagaa gatattgatc acgctgttgg attgaaactg cataaaaaaa ttggtgacac
     6001 tgtgacaaaa ggtgagtcac tattgacgat ctatagtaac gacgaagaaa tcacttctgt
     6061 cattgaatta ttatataaaa atattgaaat tggtgaatcg gcaatgaagc caacgttgat
     6121 tcatgacatc atcacagaat aaaggaaaaa agcggacttt accgttttaa agaaaagggg
     6181 aaaagaaatg gaactaaacc gaatgatcga ccatactatt ttgaaagcag acgcatctaa
     6241 agaagctgtg atgcaaatta tcgaagaagc aaaaaaatac catttttatt ctgtctgcat
     6301 caatccagca tgggttgcgc tagcgaaaga acaattacaa ggtgagccag tagccgtatg
     6361 tacagtaatc gggtttcctc taggagcaaa cacacccgag acaaaagcat ttgaaacaac
     6421 agatgccatc aataacggtg cagatgaagt cgatatggtc atcaatatcg gtgctttgaa
     6481 atcaaaggat gatcaacaag tccaaaaaga tatcgaagca gttgttgaag cagcaaaaga
     6541 tcgtgcgctt gtcaaagtta ttattgaaac atcgttatta gatagagaag aaatcatccg
     6601 agcttgcgaa atagcaaaag ctgcaggcgc tgattttgtc aaaacatcaa ctggtttctc
     6661 aacaggcgga gcaaaggtag aggatgtccg tttgatgcgt gaaactgtag gaccagaaat
     6721 gggtgtcaaa gcttccggcg gtatccataa cgaagaagaa gcaatggcaa tgatcgaagc
     6781 tggtgctaca agaatcggaa caagcgcagg agtagcaatc gtatctggtt caactggtga
     6841 aggctattaa ttttttcaaa ggagaagctc atggcagaac tcaaacaaga atggatcaac
     6901 acagctattg aagcgttaga taaagcctat gttccttatt cccattttcc ggtaggcgct
     6961 tgtctcgtaa cggaatcagg caagatttat caaggcatca acattgagaa tgcatcattt
     7021 ggattaacga attgtgccga gcgcacagct tttttcaaag cagtatctga aggagaaaaa
     7081 acatttacgc atctagtcgt agcggggcac acccccgatc ctatctctcc ttgtggtgca
     7141 tgtcgacaag taatggcaga attctgttcc cctgatatgc cggttacgct agtaggagac
     7201 aatggtgtga cgaaggcaac gaccgttcgt gcactgctgc cttatgcatt tactgaaaaa
     7261 gatctataac tcatgttcat tcagtcagct gactgttgaa aaataaaaca attattttag
     7321 gaggctttac agaaatgaaa aaagcaaaat tatttggttt aggtgctgtc gcactagcag
     7381 cagggttatt tttaggtgca tgcggcaaca acggctctac tgattcatcc ggtggcaaaa
     7441 gctcatcaga cacaacaact gcagcattga tcacagacac aggtggtgta gacgaccgtt
     7501 cattcaacca gtcagcttgg gaaggactag aaaaatgggg gaaagatcaa ggactttcac
     7561 gaggaaatga tggcttccaa tatttccaat cttcaaatga atcagattac atcccaaata
     7621 ttgaccaagc attgaatgct ggattcaaaa cgatttttgg tattggttat aaactaaaac
     7681 cagcgatcca agaacaagca acaaacaata caggtacgaa ctttgttatt atcgatgatg
     7741 tcatcgatgg attagataac gttgtatctg caactttcaa agacaacgaa gcttcttatc
     7801 ttgcaggtgt tgcagcagct tatacgactg aaacaaacgt agtcggcttt atcggcggtg
     7861 taaaaggtga agtaatcgat cgttttgatg ctggattcaa agccggtgtt gatgcaggtg
     7921 cgaaagaatt aggaaaagaa atcaaagtat tgaatcaata tgctggtgac ttctctgctc
     7981 cagataaagg acgttcaatt gctcaaggta tgtatgcaca aaacgcagat atcatcttcc
     8041 atgcttcagg cggtacaggt aacggtgtat tccaagaagc aaaatcattg aacgaatcag
     8101 gcgacaaaaa agtttgggtt atcggtgttg accgcgacca atcagacgaa ggcgaataca
     8161 cattaaacgg cgaaaagaaa aacttcacat tgacatctac actgaaagca gttggaacag
     8221 tagtagaaga tttggcacaa aaatcagcgg acggtaaatt ccctggcggc gaacacactg
     8281 tttatggatt gaaagaagac ggtgttggtc taacagaagg acaactttct gacgaagcga
     8341 aaaaagcagt tgatgaagca aaagaaaaaa tcatttcagg tgacgtaaaa gttccagaaa
     8401 ctcctgaaga aaactaaaac catttctata gccttgttag acacatgttt gacaaggcta
     8461 tggtttgatt agaacaagaa ttgttcattt atccaagctg ccttgtgaca gtttggataa
     8521 gggaacaact ccctgaagta atgtttgttt caaaagaaga acgctcacta agcgtgaatc
     8581 ttagtcaatc gttgccctag atagaaatcg ttaggatgtg aaaaaaaggt ggttcaagaa
     8641 aatattgtca tagaaatgca gaatattacc aaagaatttg gaacattcaa ggcaaatgac
     8701 aacatcaatt tacaagtaaa ggcaggagaa attcatgcgt tgcttggaga aaacggtgct
     8761 ggcaaatcta cattgatgaa cgtgctttcc ggattattag agccgacatc agggaaaatt
     8821 ttgatgcgtg ggaaagaagt aaagatcaca agtccgacca aagccaacca attagggatt
     8881 gggatggtcc atcagcactt tatgcttgtt gatgccttta ctgtaacaga aaacatcgtg
     8941 ttgggaagcg agcctagtcg cgcagggatg cttgaccata aaaaagcacg aaaagagatc
     9001 caaaaagttt ctgaacagta tggattgtca gtcaacccag atgcttacgt tcgtgatatt
     9061 tcagttggaa tggaacaacg ggtagaaatt ttaaaaacac tttaccgagg agcagatgta
     9121 ctgatttttg atgagccgac agctgtattg acccctcaag aaattgatga attaatcgtg
     9181 atcatgaagg aattagtcaa agaaggcaag tcgatcattt tgattacgca taagttagat
     9241 gaaatcaaag cagtagctga ccgctgtaca gttatccgcc gtggaaaagg aatcggtaca
     9301 gtcaacgtta aagacgtaac ctcacaacaa ttagccgata tgatggttgg aagaacggtt
     9361 tcattcaaaa cgatgaaaaa agaagcgaag cctcaagaag tcgttttgtc tattgaaaat
     9421 ctagtggtga aagaaaatcg tggattagaa gccgtgaaaa acttgaactt agaggttcgt
     9481 gctggcgaag tacttggtat cgctggaatc gatggaaacg ggcagtcgga gttgatccaa
     9541 gctttgactg gtttgcgaaa ggcagaaagc ggacatatca agctaaaagg ggaagacatc
     9601 actaataaaa aaccccgaaa aatcactgaa catggtgtag gacacgtgcc agaagaccgt
     9661 cataaatacg ggttagtcct agatatgaca ttatctgaaa atatcgcact acaaacctat
     9721 catcaaaaac cttacagtaa aaacggcatg ctgaattatt cagtaatgaa tgaacatgcc
     9781 agagaattga tcgaagaata tgatgttcga acaacgaatg aacttgttcc cgcaaaagct
     9841 ttatcaggcg gaaatcagca aaaagcaatt atcgctcgtg aagtcgaccg agatcctgat
     9901 ctgttgattg ttgcaaatcc aactcgtgga ctggatgtag gtgcgatcga atttattcat
     9961 aaacgtctga tcgaacaaag ggacaaatac aaagcagtgt tactgatcag tttcgaatta
    10021 gaagaaattt taaatgtttc ggatcgtatt gctgttatcc atgaaggaga aattgtcggt
    10081 atcgttgatc cgaaagaaac atctgaaaat gaattaggtc tattgatggc gggctatacc
    10141 ttagaagaag cacgaaaaga gttagaaaag ggggctggcg cacatgaatg atcgcagcga
    10201 aaaattccgt aatttattag tgcctgtact ttcagttgtt ttaggattga tcttaggcgc
    10261 gatattgatg gctgcttttg gttttcagcc catccaaggt tattcttcta tgctagatgc
    10321 agcccttggt tcgcaaagag ggataggtga aacgttaagg caagcaacgc cgttgatttt
    10381 tacagcatta ggtttttctg tagctaactc agctgggttt ttcaatattg ggctttctgg
    10441 acaagcactt tgcgggtgga tcgtcagtgt ctgggtggca cttgcgtttc cagatctgcc
    10501 aaaagttgtt ttgcttccgt tatgcgtgat cttaggtgcc ttagctggtg cgatggcagc
    10561 ggctgttcca ggtttacttc gagcattttt tggaacaagt gaagtgatcg taacaatcat
    10621 gatgaactat atcttattat acgtaagcac atttgcttta catgaagtga tgcctgaaag
    10681 ctatcgctca agtttggact cttccaatat gatcagtcaa aatgcctcac tgcggttacc
    10741 atggttaaca caagcttttg gcggctctcg cgtaaactca ggattatttt tagcaatcat
    10801 cgcgcttatt ttcgtttggc tgctgatgaa gaaaacgact ttaggatttg aaatccgttc
    10861 tgttggattg aatccttttg cttctgagta tgcaggaatg agtagtaaac gtacgatcgt
    10921 tttgtcgatg gtcatttctg gcgctttggc agggcttggc ggtgtggttg aaggattagg
    10981 aacgtatcaa aacttctttg ttcaaacgac ttcgctatct attggatttg acggaatggc
    11041 tgtttccttg cttggttctg gatcagcaat cggtattctt ttatcagcga tcttgttcag
    11101 tattttgaag atcggtggac ttggcatgca gacaggagca ggtgttccgt ttgagatcgt
    11161 caatgtatcg attgctttga ttatcttctt tgtcgggatc aacttcctga ttcgtttcgt
    11221 catggctaaa gtcttccaag ggaaaaaaca agaagagatc gttgcgacaa tagaatcgaa
    11281 accaacgaat caaaaccatg aagggggcga actataatgt cgactatcga attaattgcc
    11341 ttgatcctta cttctacact ggtttattca acaccgctaa ttctaacgtc actaggcgga
    11401 actttttctg aacgaagcgg gatcgtaaac gtagggctag agggaatcat ggtcatgggt
    11461 gcttttagtt ccatcgtttt caacttgacg atgagtccat cacttggttc tatgacacca
    11521 tggatcgcta ttttagtggg tggcgtcgta ggtatcctat tttctctgat tcacgcagta
    11581 gcaacagtca gtttgcgtgc tgaccatatt atcagcggga cagtaattaa tctgatggca
    11641 cctgctttag gtgttttctt gatcaaagca tggtatggta aaggtcaaac tgataacatc
    11701 acgcagaatc tggggtattt ttctttccca ggattagcag atattccggt cattggacca
    11761 attttcttta aaaatacatt cttacctgct tattgtgcta ttttgattgc tgtcctagct
    11821 tgggtgatca tcttcaagac gaaattcggc ttgcgcctcc gttcagttgg tgaaaatcct
    11881 caagctgccg acacattagg tatcaatgta tatgctatgc gttattcagg cgtactgatt
    11941 tctggcttgt taggcggtat gggcggtgct gtttttgctc agtcgatttc cggtaacttt
    12001 tcagctggta cgatcgttgg acaagggttc atctccatgg ctgctatgat ctttggtaag
    12061 tggaatcctt taggggcaat gggtgcctca ttgttctttg gttttgctca aagcttgagt
    12121 atcattggcg cacaattacc agttatttct tcgattccgc cagtattgct gcaaattgct
    12181 ccatatttgc tgacaatcat tgtgctggtt gttttcttag ggaaagcttc tggtccaaaa
    12241 gcaaacggga agaactatat caaatcaaaa taatctttta gcgaataaaa agggtgggtt
    12301 acgggaagtg tttttcgtct cgcccttttt gttttaaagg caataaaaat aaaaagaaag
    12361 aaggaataat catgtttaaa cgcgtacatc tgatcgtcat ggattcagta ggaattggag
    12421 aagcaccaga tgccgataag ttcggtgatg ttggttctga tacattagga cacattgcga
    12481 aagaagcagg attgaacgtt ccacatttag agcagcttgg tttaggaacg atccgtcctc
    12541 ttgaaggtgt ggaatcagtg aaagatcatc aaggctatgc gacgaaatta gaagaagttt
    12601 ctgtagggaa agatacgatg acaggccact gggaaatcat gggattgaat atcaaaactc
    12661 catttcgtgt gtttcctaat ggattccctg atgatctgat caaacaaatc gaagaatttt
    12721 ctggccgtaa agtcgtatgt aacaaacctt atagcggaac agctgttatt gacgattacg
    12781 gtgaacacca aatgaaaaca ggcgatttaa ttgtatatac ttctgccgat cctgtccttc
    12841 agatcgcggc acatgaagaa atcattcctt tagaagaatt atatcggatt tgccaattcg
    12901 ttcgtgatat aacaaaagat gatccgtata tgattggccg aatcattgca cgtccgtacg
    12961 tgggtgaacc aggagacttc acacgaacaa gcaatcgcca tgattatgcg ttgaatccat
    13021 tcggacacac agtccttgat tctttgaaag aagcaggaaa ggacgtgatt gctgttggaa
    13081 agatcaacga tattttcaac ggacaaggaa tcactgaatc tgtccgcacg aaaagcaata
    13141 tggatggcgt tgaccagctg ctgaaagtta tgaaaaacga tttttcagga atcagcttta
    13201 ccaatttagt agattttgat gcattatacg gtcatcgtcg ggatgtagtc ggctatgcgc
    13261 atgcgattga agaatttgac ttacgaattc cagaattgct tgaagcgatg gaagaagacg
    13321 acttgctaat gattacagcc gatcacggaa acgatccgac gtttactggt accgatcata
    13381 cgcgagaata tgtaccgtta ttggtttaca gtaaaaaaat gaaagaacaa ggggcattgc
    13441 ctcaaggata ttattcagat atttcagcaa cgattgctga gaactttagt gtttcttcga
    13501 ctgaaaatgg cgaaagcttc ttgaaattat taaaataatc tttttaaata ggagaaagca
    13561 aatgaccaag ttaagcgaga tgttacaaga aacgacccag tttatccttg ataaaggagt
    13621 aaaagaaatt gattttggtt tgattctagg ttcaggatta ggtgaattag cagatgaaat
    13681 cgaagatgct gtaatgattt cttatgatga gatcccgttc tttcctgttt cgacagtagt
    13741 tgggcatgca ggaaagctag tttatgggtc tctggcaggc aagaaagtgt tagccatgca
    13801 agggagattc cactactatg aaggtcattc catgcaaaca gtgacctatc cagtacgtgt
    13861 gatggccgga ctaggggccc attcagtcat tgtgactaat gcatgtggtg gtgtcaacga
    13921 atcatttgta ccaggagatt tgatgctgat taccgatcat atcaatttta ctggtgaaaa
    13981 tcctttgatc ggtccgaatg aagatgcaat gggccctaga tttccagata tgagtcaagc
    14041 ttatacgcct gactatcaag caacagcaaa agcagcagca gaaaaactag gtttggatct
    14101 taaagaaggc gtatatatgg gctattctgg accaacttat gaaacgcctg cggagatccg
    14161 aatgtctcgg acaataggag ccgatgcagt agggatgtcg actgttccag aagtcatcgt
    14221 agcagctcac agcggattaa aagtgttagg aatctcttgt attaccaact tagcagcagg
    14281 tatgcaagcg aatctgagcc atgaagaagt tgtagaaaca actcagagag tcaaacaatc
    14341 ttttaaagca ttggttaaag aaacattgac attattgtaa taggaggaac aataaaatga
    14401 gtgtgcatat tgaagcgaaa gaaggcgaga tcgccgataa gattttactt ccgggagatc
    14461 ctttgcgagc taaatatatt gcggagactt ttttggaaga tccagtttgt tacaatcagg
    14521 tacgcggtat gttaggcttt accggcaaat ataaaggaga acgtatttct gttcaaggaa
    14581 caggaatggg aatgccatca gccaccatct atgcccatga attgatccag tcgtatggtg
    14641 taaagaaatt gattcgagta ggtacatgcg gtgcattgtc aaaagacgtc catgttcgtg
    14701 atttagtact agcacaaggt gcagcgacca gttcttcgat gatcgaaaaa aatttccaag
    14761 cctttcattt tcctccaatc agtgatttca atctattact gaaagcatat gagatagcta
    14821 aagaaaaggg ctatacggtt catgtaggaa atgttctttc ggaagattct ttctacaaag
    14881 atgatttgac agagacgttc caattagctg aacttggcgt tttaggagtg gaaatggaag
    14941 cagcggctct ttattactta ggcgcgaaat accatgtgca gacattaagt ttgatgaccg
    15001 ttagcgacca tttgatcacc ggtgaagaaa caacagctgc agaacgtcaa tctacgttca
    15061 atgaaatgat cgaagtagga ttggaaacag cgatagcaga ataagaagga aatgaaaaag
    15121 ctgccctgca ttaagtaata agaacatcca agcaaaaata gctcctcatg tttttgactt
    15181 ggctggactt attattgcaa atgtaagctt ttgaacaccg tttaccaaga ggttgtgaat
    15241 ctgccgttta actctgagtc acaaggaaca atttcaaaaa acagcttttc atgtttgatg
    15301 aatattgtga cttgtgaccg agaagttggc aggagaaaac cgtttatacg aaaaaaggga
    15361 ctgtgacaaa tcgtgtcaca gtcccttttt tatagtgttt tttataggta gtatttttta
    15421 gcaactgtta agtcatcatt taattcataa acaagtggtt gacctgttgg aatttcaaga
    15481 tccatgatgt cttcgtcaga gataccttcg atatgtttag ctagggcgcg taatgagtta
    15541 ccatgtgcag ctactaaaac agttttgttg tctagtaatg ctggagcaat ttcatcttgc
    15601 cagaatggca aagcgcgttc taatgtcact ttcaagtttt ctccaccagg aatatcgcgt
    15661 ttgtctaaca ttgcgtaacg acggtcattt gctgcagaac cttcgtcagt ttcttccatt
    15721 aatggaggaa gagtatcgta agaacgacgc cagatgtgca cttgttcgtc gccgtatttt
    15781 tcagctgttt cttttttgtt caaaccttgt aatttaccat agtgacgttc gtttaagcgc
    15841 catgatttga tttgtggtac ccaaagttga tctgaatatt ctaagatcaa gttacatgtt
    15901 ttgattgcac gagttaaaac agaagtgtaa gcaacatcaa attcgattcc agcttctttg
    15961 attttgcgtc cgccttcttt tgcttcttcg attccttctg gtgctagatt tacatcagcc
    16021 caaccagtaa attggttcaa tgcattccat tcactaagac cgtgacgaga aaaaactaat
    16081 tttggcatgt ttagctctcc ttttaagata ggtttatatt tctgaaattt tcagttctgt
    16141 tttattgtac aattttttgg aagagatttc catcgaaaaa ttagataaga ttctatgaga
    16201 aaacaagtag agaatataaa aaaacgaaca gtgcccacgc aactgttcgt ttttaggtat
    16261 tagatgcata attgctcgat ttctttttca ggtaaattga tatatttata acatgttcca
    16321 actgaaacac cgcattttga agaaataaat tggatcgttt cttttttttc ataatagaga
    16381 cgtcggattt ttttgatcgt ttttgcatcg atttttggtc ttccgccgat ttttcctttt
    16441 ttacgagctt catctaatcc aactaatgta cgttctttga tcaatgcgca ctccatgttt
    16501 gctaggtttt ccattaattg gaaatagatt tttcccattt cgcgcgtatc gattccttca
    16561 tcaagactaa tcaagtgcag cccgcgatct ttgaataatt gcgtgagttc tgttaattgc
    16621 cgcgtagatt tgcctaatcg atgaagttgg caaatgacta gtgcatcgcc ttgttgcatg
    16681 tcagcgatca ctgactccaa atgaacagca gtagaatcat tttgttccac ttcatatgtc
    16741 tcatggaaaa tttcttgaca accaaagtca gataattgat caacctgagt tgttaaatca
    16801 acgtcattta tggttgtgcg tgcataacca ataattttca tggtgattcc tttctagcag
    16861 tgattttcta actggatata acagtaaaaa tgtagcataa agtacgtcaa aaagatagtt
    16921 ttttcgcaaa ttttccgctg tcttttttta ttggatgatc aagcagtttt tctttattta
    16981 cgacggattg cgtcaagaat ctcaacgcct ttttctgagc cgacaactac tttagaaacc
    17041 atgtttagga aaagcccatg ttctactacc ccaacaagtt catctaaata ggtagctaag
    17101 ctttctggct gttcgatgac ttctagatgc agatcaatga tgtaatgacc accatctgtg
    17161 gttaagactt ctccgttttc gtctgtacgc aagacgggtt ggaatctttt ttcttcaaaa
    17221 atatgaagta gctgttggga gccataaggg atcacttcaa ctgggagtgg gaacttccca
    17281 agtttatcga ctaatttaga agaatcaacg atccaaatcg tttcttttga gtacgtagcg
    17341 actattttct cgaaaagcaa agcagcgccg ccgcctttga tcccttgaaa atcagcactg
    17401 atttcatcag caccgtctat tgtcaaatcg actacgggga cttcatcaat actctttaac
    17461 ggtattccta atttttccgc ttgttctttt gtcgcatttg aagtagtaac tcctgtgata
    17521 cgtaaacctt cttcacgcat acggcgacca atctcttcta ccatataata ggcggttgaa
    17581 ccggtcccga gacctacgat catcccgtcc tttacgaatt tcgcggcttc aatgccagct
    17641 aattgtttta aattcatctt tgcgtccacc tttttttagt ttgagcatcg tgctttcctc
    17701 actattttag cgtgatttac ggtaaaaaga taccattctt ttttattcca ataaagcttt
    17761 ttcaaaaaaa attgcatctc ttgttgacat gttttttcat tcgtgatatt ctaacaaagg
    17821 tgctttatct acaggtttct gtgaagaaac gtgctgactg tttatcaaag catatttgat
    17881 aaggatgcag acattgcatt tattttttat cacaaaaaaa gaaccacctg gatgtgtgga
    17941 actagacgca aatcgtggga aggaggaaac aaggaatgcc tacaattaac caattagtac
    18001 gtaaacctcg taaatcaaag gtggaaaaat ctaattcacc tgctttgaac aaaggatata
    18061 acagctttaa gaaaactcaa actaacgtaa actcacctca aaaacgtggg gtagcaactc
    18121 gtgtgggtac aatgacacct aaaaaaccga actcagcttt acgtaaatat gcacgtgttc
    18181 gtttgtcaaa cttaattgaa gttactgctt atatcccagg gattggtcat aacttacaag
    18241 aacacagtgt tgtattatta cgcggtggtc gtgtaaaaga cttaccaggg gtacgttacc
    18301 atatcgttcg cggtgcttta gatactgctg gtgttaacga ccgtaaacaa agccgttcta
    18361 aatacggaac taaaaaacct aaagcttaat tttaattatt aaacaaatga aacttgcttc
    18421 tactatatag aagtaattac tatgaacaat atttcggaag gaggagttac ggatgccacg
    18481 taaaggtcct gttgcaaaac gtgatgtttt accagatcct atttataact caaaattagt
    18541 aactcgcttg atcaaccgtg taatggttga cggaaaacgc gggattgcag ctaacattat
    18601 ctataatgcc tttgacttga tcaaagaatc tacaggtaac gatccattag aagtgtttga
    18661 acaagcaatg aaaaacgttc aacctgttct tgaagttaaa gcacgtcgtg ttgggggttc
    18721 aaactaccaa gtaccagttg aagttcgccc tgaacgccgc gttactctag gtcttcgttg
    18781 ggtagtaaac tacgctcgct tgcgcggtga acacacaatg gaagaacgct tagcaaaaga
    18841 aatcatggat gctgcaaaca acacaggtgc ttctgttaaa aaacgtgaag acactcataa
    18901 aatggctgat gccaaccgtg cgtttgctca ctatcgctgg taagattctc tctgtttggt
    18961 cggcttttat aagtcgacct cagatagtgt ccttattttt agcaactaga tagtaaagag
    19021 aggagaaaca aggagaatga ccagagaatt ttctttacaa aacactcgta atatcggtat
    19081 catggcccac atcgatgctg gtaaaacgac tgctactgaa cgtatcttgt actatactgg
    19141 taaaatccat aaaattggtg aaactcatga aggagcttca caaatggact ggatggaaca
    19201 agaacaagaa cgtggtatta cgatcacttc tgctgctact acagcacaat ggaagggtca
    19261 ccgaatcaac atcatcgaca caccaggaca cgtagacttc acagttgaag ttgaacgttc
    19321 attgcgtgta cttgatggtg ctgtaactgt tcttgatgca caatcaggtg ttgaacctca
    19381 aactgaaaca gtttggcgtc aagcaacaac ttatggtgta cctcgtatcg tatttgcaaa
    19441 caaaatggac aagatcggtg ctgacttctt atattcagta tcaactttac atgaccgttt
    19501 gcaagcaaac gctcacccaa tccaattacc aattggtgcg gaagacgact tcacaggaat
    19561 tatcgacctt gtaacaatga aggctgaaat gtatacaaac gatcttggaa cagagatcga
    19621 agaaacagaa attcctgaag aataccgcga attagctgaa gaatggcgcg aaaaattggt
    19681 tgaagctgta gctgaaactg atgaagaact tactttgaaa tacttagaag gtgaagaaat
    19741 cactgaagct gagttaaaag aaggtatccg ccgcgcaaca gttaacgtag aattctaccc
    19801 agtactatgt ggttcagcat ttaagaacaa aggggttcaa ttactattgg acgctgttct
    19861 tgattacttg ccatcaccgt tagatatccc agctatcaaa gggattgacc ctaaaactga
    19921 tgaagaagta gaacgtcctg cagacgacag cgctcctttc tcagctttag catttaaagt
    19981 tatgactgac ccattcgtag gtcgtttaac tttcttccgt gtgtactctg gtgttttaca
    20041 atcaggttca tacgtacaaa acgcaacaaa aggcaaacgt gaacgtgttg gtcgtatcct
    20101 acagatgcat gccaactctc gttcagaaat ctctgaagtt tacgctggag atatcgctgc
    20161 tgctgttggt ttgaaagaca caactactgg ggacacacta tgtgatgaaa agagtctagt
    20221 tattcttgaa tccatggaat tcccagaacc agttatccaa gtcgctattg aacctaaatc
    20281 aaaagctgac caagataaaa tgggtgtggc tttacaaaaa ctatctgaag aagatcctac
    20341 tttccgtgct gaaacaaacg tggaaacagg cgaaacaatc atcgctggta tgggtgaact
    20401 tcacttagat atcatcgttg accgtatgcg tcgtgaattc aaagttgaag ctaacgtagg
    20461 tgcgcctcaa gtatcttacc gtgaaacttt ccgtgctggc actcaagccg aaggtaagtt
    20521 tgtacgtcag tctggtggta aaggtcaata cggtcacgta tggattgaat ttacaccaaa
    20581 tgaagaaggt gctggtttcg aatttgaaaa tgcgatcgtc ggtggggttg ttcctcgtga
    20641 atacattcca gctgttgaaa ctggtttgaa agatgctatg gaaaatggtg tcttagctgg
    20701 ctatccatta gtagatatca aagctaaact ttacgatggt tcataccatg atgtcgattc
    20761 aaatgaaact gccttccgtg ttgctgcttc tatggcatta cgtgcagcag ctaagaaagc
    20821 taatccagta atccttgaac caatcatggc tgtagaagtt gttatccctg aagattattt
    20881 aggtgatgtt atgggtcacg taactgctcg tcgtggacgt gttgaaggta tggaagctcg
    20941 cgctggcggc caacaagttg ttcgtgcgat ggttccactt gctgaaatgt ttggttatgc
    21001 tacaactctt cgttctgcta ctcaaggtcg tggtacattt acaatgacat ttgaccacta
    21061 cgaagatgta ccaaaatcag tacaagaaga aatcattaag aaaaacggcg gaaaagctga
    21121 ataatttctt aaaaccggat agtcctgcaa agagattatc caaagagact atatttttaa
    21181 tatagtttcg tgtaaaataa ttatcgatga tatgggcatg tggtgtacct tgtgtacatc
    21241 atatacctat cagacaaata aaaaatatat tgtattttta ggaggacatt ttaaaatggc
    21301 aaaagaaaaa tttgaccgtt ctaaaccaca cgttaacatt ggtactatcg gacacgttga
    21361 ccatggtaaa actacattaa cagctgcaat cacaactgta ctatctaaga aaaacggcgg
    21421 ccaagctatg gcttacgatc aaatcgatgg tgctccagaa gaacgcgaac gcggaatcac
    21481 aatctcaaca gctcacgttg agtatgaaac agatacacgc cactacgctc acgttgactg
    21541 cccaggacac gcggattacg ttaaaaacat gatcactggt gctgctcaaa tggacggagc
    21601 tatcttggta gtttctgctg ctgacggccc aatgcctcaa actcgtgaac acatcctatt
    21661 gtctcgtcaa gttggtgttc cttacatcgt tgtattcttg aacaaagtag acatggttga
    21721 tgacgaagaa ttactagaat tagttgaaat ggaagttcgt gacctattaa cagaatacga
    21781 attccctggt gacgatgttc ctgtagttgc tggatcagct ttgaaagctc tagaaggcga
    21841 cgcttcatac gaagaaaaaa ttcttgaatt aatggctgca gttgacgaat acatcccaac
    21901 tccagaacgt gacaacgaca aaccattcat gatgccagtt gaagacgtgt tctcaattac
    21961 tggacgtggt actgttgcta caggtcgtgt tgaacgtgga caagttcgcg ttggtgacga
    22021 agttgaagtt gttggtattg ctgaagaaac ttcaaaaaca acagttactg gtgttgaaat
    22081 gttccgtaaa ttgttagact acgctgaagc tggagacaac attggtgctt tactacgtgg
    22141 tgttgcacgt gaagacatcc aacgtggaca agttttagct aaaccaggta caatcacacc
    22201 tcatacaaaa ttctctgcag aagtatacgt gttgacaaaa gaagaaggtg gacgtcatac
    22261 tccattcttc actaactacc gtccacaatt ctacttccgt acaactgacg taacaggtgt
    22321 tgttgaatta ccagaaggaa ctgaaatggt tatgcctggc gacaacgtaa caatggaagt
    22381 tgaattaatc cacccaatcg ctatcgaaaa cggtactaaa ttctcaatcc gtgaaggcgg
    22441 acgtacagtt ggtgccggtg tcgttacaga aatcaaagct taattcactt aagttttaaa
    22501 gtaaagcaac atttattcgg acgtaatcgc tcggaacgaa ggtcttagga tctttgttcc
    22561 gagctttttt atttattgca ggatcaggaa aaaagacagg agtattataa atagaagaaa
    22621 cacgaaataa aagagtcagt attttctaaa tgaaacttta tgaaaacaaa atgtttttta
    22681 caagaaaaag ccttgctttg tgagtttata tatattataa tgacaaaggt gcgtttactt
    22741 tagtgaattg cacagacgta atcagaatga tttcgtcggc taagaaacga gaggttgcga
    22801 cacgcccgga cgctttgcca cgaacgagcg tgacggaaat tttcgtggag ctatgtctac
    22861 ttttcaaaat aggcgaagga gggaacaaga tggcaaaaca aaaaattcgt atccgtttaa
    22921 aagcgtatga acaccgtatt ttagatcaat cagcggataa aatcgtggaa actgcaaaaa
    22981 gaactggagc tagcgtgtct ggtccaattc cattaccaac tgaacgcagt ctttacacaa
    23041 tcatccgtgc gactcataaa tacaaagatt cacgcgaaca attcgaaatg cgtacacaca
    23101 aacgtcttat cgacattgtg aacccaacac caaaaactgt tgatgctttg atgaagctag
    23161 acttaccatc tggtgtaaac atcgaaatca aattataaaa ctaaaacaaa aataatggag
    23221 gtgtactcat gaccaaagga atcttaggga aaaaagtggg aatgacacaa atcttcactg
    23281 aatctggtga attaattcca gtaactgttg tagaagctac tccaaacgta gtactacaag
    23341 tgaaaacaat ggaaacggat ggctacgaag ctattcaagt tggttaccaa gatatgcgtg
    23401 aagttttatc aaacaaacct gcgaaaggtc atgttgcaaa agcaaacacg gctcctaagc
    23461 gcttcattcg tgaattcaaa aatgttgagc taggagaata tgaagtagga aaagaaatta
    23521 cagtagatgt tttccaagca ggagacatta ttgatgtaac aggtactacg aaaggtaaag
    23581 gattccaagg ggttatcaaa cgccacggac aaagccgcgg accaatggct cacggttctc
    23641 gttaccatcg tcgtcctggg tcaatgggtc cagttgcacc taaccgtgta tttaaaaaca
    23701 aacgtcttgc tggccgtatg ggtggcaacc gcgtaactat ccaaaacttg gaagttgtac
    23761 gtgtcgatgc agaaagaaat gttatcttga tcaaaggtaa tgttcctgga gctaaaaaat
    23821 ctttaattac tatcaaatca gctgtgaaag ctaaataatt gtaggaagaa aggaggaact
    23881 aaggaatgcc gaatgtagca ttattcaaac aagatggaag ccaaaacggt gaaatcacac
    23941 ttaacgaaga aatcttcgga attgaaccta atgaaagtgt tgtttacgat gcaatcgtaa
    24001 tgcaacgtgc gtcattaaga caaggaacac acgcggttaa aaaccgtagc gctgttcgcg
    24061 gcggcggacg taaaccatgg cgtcaaaaag gaacaggtcg tgcacgtcaa ggatctatcc
    24121 gctcaccaca atggcgtgga ggtggcgtag tctttggacc aacaccacgt tcttacagct
    24181 acaaacttcc taaaaaagtt cgtcgtttag caatgaaatc tgtattgtca gaaaaagttg
    24241 ctgaaaacaa cttggtagca atcgaaggct tgaacttcga cgcaccaaaa acaaaagaat
    24301 tcaaacaagt tcttgctaac ttatctatcg actctaaagt attggttgtt ctagaaaaag
    24361 gtaacgactt tgctgcttta tcagcacgta acttaccaaa cgtttctgta gtaacttctg
    24421 ataatgtgag tgtgctagac gttgtatcaa atacaaaagt gttagcaaca caaactgctc
    24481 ttactcaaat tgaggaggtg cttgcataat gaacttacta gacgtaatca aacgcccagt
    24541 gatcactgaa aaatcaatgc ttgccatgga tgacaagaaa tacacttttg aagtggacac
    24601 tcgcgcaaac aaaactttag taaaacaagc ggtagaagct gcttttgacg ttaaagtcaa
    24661 aaacgtgaac atcgtgaacg tacgtgcgaa attcaaacgt atgggcaaat atgcaggata
    24721 tacaaaaaaa cgtcgcaaag cgatcgttac attgactgaa gattcaaaag aaatccaatt
    24781 attcgaagct gctgaataag cactaataga taactcatta aattaggagg gaaaaacgtg
    24841 gcgattaaaa agtacaaacc tacctcaaat ggtcgtcgta acatgacagg ttctgatttt
    24901 gctgaaatca caacatcaac acctgaaaaa tcattgttac aaccattgaa aaaccatgct
    24961 ggacgtaaca acaacggccg catcactgta cgtcatcaag gtggcggaca caaacgccaa
    25021 tatcgcgtga tcgacttcaa acgtaacaaa gataacgttg ttgcaactgt tcaaacgatc
    25081 gaatatgatc caaaccgttc agctaacatc gcgttagtac actatgaaga tggagtgaaa
    25141 gcttacatct tagcacctaa aggactagaa gtgggtatga aacttgtttc aggtccagaa
    25201 gcagatatca aagtcggaaa tgctcttcca ttagaaaaca ttccagttgg tactgttatc
    25261 cacaacattg aaatgaaacc tggtaaaggt ggacaattga tccgttctgc tggtacaagt
    25321 gctcaagtac ttggtaaaga aggcaaatac gtattgatcc gcttgaactc tggtgaagtt
    25381 cgtatgatct tagctgcatg ccgtgctaca atcggttctg taggtaacga acaacacgaa
    25441 ttaatcaaca tcggtaaagc tggacgctct cgttggatgc gtaaacgccc aactgtacgt
    25501 ggtagcgtaa tgaaccctaa tgatcaccca cacggtggtg gtgaaggtaa agctcctatc
    25561 ggacgtaaag caccagtttc tccttggggt caaccagctc ttggttacaa aactcgtaac
    25621 aaaaaagcta aatcagacaa acttattgtt cgtcgtcgca aaaataaata attactttga
    25681 ccatgtgtcg cacattttga gaggaggttc accatgggtc gtagtttaaa gaaaggacct
    25741 tttgtcgatg agcatttaat gaaaaaagtt gaagctcaag caggggttga aaagaaaaaa
    25801 gttatcaaaa cttggtcacg ccgttcaaca gttttcccaa gttttgttgg atataccatc
    25861 gcagtttatg atggacgtaa acatgtacca gtttacatcc aagaagacat ggtaggacac
    25921 aaattaggtg aatttgcacc tacaagaact tatcgtggac atgctgctga cgataagaaa
    25981 acgaaacgct aatttgaggg gaggataaat ccaaatggca gaacaaatca catcagctaa
    26041 agcaactgca aaaacagttc gcacttcacc tcgaaaagcc cgtttagtaa ttgaccttat
    26101 cagaggtaaa agtgtcgcag atgcaatttc aatcttgaaa ttcacaccaa acaaatctgc
    26161 tggaatcatt gaaaaagtat tgatgtcagc agttgcgaat gcagaaaata actttgactt
    26221 agatgttgaa aacttggtag taagcgaagc ttttgttaac gaaggaccta caatgaaacg
    26281 tttccgtcct cgtgcaaaag gatcagcttc tccaatcaac aaacgtacaa gtcatatcac
    26341 ggtagtcgta tcagaaaaat aaggagggat aatcagtggg tcaaaaagta catccaattg
    26401 gaatgcgtgt aggcatcatc cgcgactggg atgctaaatg gtatgctgaa aaagagtatg
    26461 ctgagttctt acacgaagat ttaagaatcc gtaaatttat ttcaactaaa cttgctgatg
    26521 ctgctgtatc aacagttgaa atcgaacgcg caacaggtcg cgtaaacatt tcaattcaca
    26581 cagctaaacc aggtatggtt attggtaaag gcggttctga agtcgaaagc ctaagaaaag
    26641 aattaaacaa attaactggt aaaagaatcc atatcaatat cgtggaaatc aaaaaaccag
    26701 atctagatgc aaaattagta ggtgaaggaa tcgcacgtca attagaaaac cgtgttgctt
    26761 tccgtcgcgc tcaaaaacaa gccatccaac gctcaatgcg tgctggcgct caagggatca
    26821 aaactcaagt atctggtcgt ctaaacggag cagatatcgc tcgttcagaa ggatactcag
    26881 aaggaactgt tcctcttcat actttacgtg cggacattga ttacgcatgg gaagaagcag
    26941 atacaacata cggaaaacta ggagttaaag tgtggatcta tcgtggagaa attcttccaa
    27001 ctaaaaaaaa cactgagaaa ggagggaaat aatcatgtta gtacctaaac gtgtaaaaca
    27061 ccgtcgtgaa ttccgcggaa aaatgcgcgg ggaagctaaa ggcggaaaag aagtagcatt
    27121 cggtgaatac ggtttgcaag ctgttgattc acattggatc acaaaccgcc aaatcgaagc
    27181 tgctcgtatc gcaatgactc gttacatgaa acgtggtggg aaagtatgga ttaaaatttt
    27241 ccctcacaaa tcttatactg ccaaagcaat tggggtacgt atgggttctg gtaaaggggc
    27301 acctgaagga tgggttgcac cagtaaaacg tggtaaaatc atgtttgaaa tcgcaggcgt
    27361 tcctgaagaa gtagctcgtg aagcgttacg tctagcttct cacaaattac caatgaaaac
    27421 taagatcgta aaacgtgagg aaatgggtgg tgaatcgaat gaaggttaaa gaaatcagag
    27481 aattaaccac tgccgaaatg cttgatcaag aaaaacaatt aaaagaagaa ttgtttaatc
    27541 ttagattcca attagcaaca ggtcaattag aaaacactgc acgtattaaa gaagtacgta
    27601 aatcgattgc acgcatcaaa actgtgttgc gtgaacaagc taagtaatta tggaaggagg
    27661 ccattaagcg tatgactgaa ggaagaaatc aacgcaaagt ttatcaaggt cgcgtggttt
    27721 cagacaaaat ggataaaaca attaccgttg ctgtcgaaac aaagaaaaac caccctatct
    27781 atggcaaacg catgaactat tctaagaaat acaaagtgca tgatgaaaac aacacagcaa
    27841 aagttggcga catcgtgaga attatggaaa ctcgtccatt atcagctaca aaacgtttcc
    27901 gtttgttgga agtagtcgaa gaagcagtta ttatctaata acacgagatt ttcctatata
    27961 gattgaatgc aaaatctgaa aggaggatac acaacgtgat ccaagcagaa agtcgtttaa
    28021 aagtcgctga taactcaggc gcacgtgaaa ttttgacgat caaagtcctt ggtggatctg
    28081 gtcgtaaaac tgctaacatc ggtgatgtga tcgttgctac ggttaaacaa gcaacgccag
    28141 gtggggttgt caaaaaaggt gacgtcgtta aagccgttat cgttcgaaca aaatcaggag
    28201 ctcgtcgtac agacggttca tatattaaat ttgatgaaaa tgctgcagtg attattcgtg
    28261 atgataaaag ccctcgtgga acacgtatct ttgggccagt tgcacgtgaa ctacgtgaaa
    28321 acaacttcat gaagatcgtt tcactagcac cagaagtttt ataatcttga gacacttatc
    28381 aaaggaggtg cgaaacagtt atgtttgtta aaaaaggcga taaagttaaa gttatcactg
    28441 gaaaagacaa aaacaaagaa ggcgttgtac tagcagcgtt cccgaaaaaa gataaagtta
    28501 ttgtcgaagg tgtaaatgtc gttaaaaaac accaaaaacc ttctcaagct gctcctcaag
    28561 gtggaattgt tgagatggaa gcgccaattc atgtttctaa cgtgatggtg gttgactcta
    28621 ctggtgtagc tggccgagtt ggctacaaag aagtagacgg caaaaaagtc cgtgtttcta
    28681 aaaaaaccgg tgaagtttta gataaataaa agtttaagga aggagggtct tactgaatga
    28741 accgcctaaa agaaaaatat cttaaagaag taactccatc attgatggaa aaatttaact
    28801 atagctctgt tatgcaaaca cctaaagttg aaaaaatcgt catcaacatg ggtgttggtg
    28861 atgcggtatc aaacgctaaa aacttagata aagcagttga agaattaact ttgatcactg
    28921 gtcaaaaacc aatgattaca aaagctaaaa aatcaatcgc tggattccgt ttacgtgaag
    28981 gaatgccaat tggtgcaaaa gttacattgc gcggagaaag aatgtacgaa ttcttagaca
    29041 aattggtttc tgtttcacta ccacgtgtac gtgacttcca tggtgtaagt aaaaaagcct
    29101 ttgacggtcg tggaaactat actttaggta ttaaagaaca attgatcttc ccagaagtag
    29161 attatgattt agtagataaa gtacgtggta tggacatcgt tattgtaaca acagcaaact
    29221 ctgatgaaga atctcgtgag ttgttgacac aattaggcat gccattccaa aaataattaa
    29281 aggaggcgaa ctacgtggct aaaaaatcaa tgattgctaa aaacaaacgt cctgcaaaat
    29341 tttcaacaca agcttatact cgttgcgaac gttgcggacg tccacattca gtttatcgta
    29401 agttccatct ttgccgtatt tgcttccgcg aacttgccta taaaggtcaa attcccggcg
    29461 tgaagaaagc tagctggtaa aaagtaaatt cgcataagaa ggaggtaaaa gatcaatggt
    29521 catgacagat ccaattgcag attttctaac tcgtatccgt aacgcaaaca tggttaaaca
    29581 tgagtcttta gaagtacctg catcaaaaat taaacgtgac atcgctgaaa tcttgaaacg
    29641 tgaaggtttc gttcgtgatg ttgaatacat cgaagatgac aaacaaggcg tgatccgtgt
    29701 tttcctaaaa tttggtaaaa acggcgaacg tgttatcaca aacttaaaac gtatctctaa
    29761 accaggtttg cgtgcttatg tcaaagctga cgaagtacca aaagtattga acggtttagg
    29821 tatcgcaatc atttcgactt ctgaaggtgt tgtcactgat aaagaagctc gtgaaaaaaa
    29881 tatcggcggc gaagttatcg cttacgtatg gtaatcaagt agaatctaca aggaggtgtc
    29941 tttaagtgag ccgtatcgga aataaagtcg ttgttatccc tgaaggagta acagttactc
    30001 aagacggaaa caacgttaca gttaaaggac caaaaggtga attgactcgt actttttcag
    30061 cagatatcaa aatgaatatc gaaggaaacg aagtaacttt cactcgtcca aacgacagta
    30121 aagaaatgaa aacaattcat ggaacaactc gtgcgaactt caacaacatg gttgtaggtg
    30181 tcagcgaagg tttccaaaaa gcgttagaac taatcggggt tgggtaccgt gcacaattac
    30241 aaggatcaaa acttgtattg aacgttggtt actcacatcc agtagaaatc acaccaccag
    30301 ctggagtaac tgttgaagta ccagctaaca cacaagtaat tgttaaaggt gcgaacaaag
    30361 aagaagttgg cgaattagct gcaaacattc gtggaactcg tcctccagaa ccttataaag
    30421 gtaaaggtat tcgttatgtt ggcgaatatg tacgccgtaa agaaggtaaa actggtaaat
    30481 aatcgtttag aaaaattgca tagaagccag cagcaggaaa aaggcatttg gctgaagttt
    30541 accttcaaaa aaatacattt ttacttgtgc cgcagcttct atttttcgaa acgtatttac
    30601 tgcttgaaat agtagtacaa taaaaataaa gaggtgacaa ttgtgattac aaaaccagat
    30661 aagaacaaaa cacgtcaaaa gagacatcgt cgtgtacgta acaatatctc tggtactgct
    30721 gagcgcccac gcttgaacgt ttttcgttct aacaaaaaca tctacgctca aattattgat
    30781 gacgtagcgg gtgtaacgct agcaagtgcc tctaccttgg ataaagaaat ttcaggtgga
    30841 acaaaaacag aagcagcaca agctgtcggt aaactagttg ctgaacgtgc agctgaaaaa
    30901 ggcattaaag tagtagtctt tgaccgtggt ggataccttt accatggccg tgtgcaagct
    30961 ttagctgaag ctgctcgcga aaatggacta gaattttaga agaaggagga ataccattca
    31021 tggtttatat cgatccaaaa catttggaat tagaagaccg cgttgttgca attaaccgtg
    31081 taacaaaagt tgttaaaggt ggacgtcgtc tacgttttgc tgctttagtt gttgtcggtg
    31141 acaaaaacgg acacgtaggg tttggtacag gtaaagctca agaagttcct gaagcaatcc
    31201 gtaaagcaat tgaagatgcg aagaaaaact tagttgaagt acctatggtt ggttcaacaa
    31261 tcccacacga agtaatcggt gcttttagtg gtggtagaat cctaatgaaa cctgctgtag
    31321 aaggttctgg ggttgctgct ggtggaccag ttcgtgccgt attagaatta gctggggtag
    31381 cagatatcac ttctaaatct ttaggctcaa atacaccaat caacgttgtt cgcgcaacag
    31441 ttgaaggttt gaaacaattg aaacgtgcag aagaagttgc agaacttcgc ggcaaatcag
    31501 ttgaagaatt aatcggataa ggaggacaac aataatggct gaattaaaag ttactttaaa
    31561 acgcagtatt atcggacgtc ctcaaaacca acgcgataca gttaaagcgt taggtcttac
    31621 aaagattaac agttcagtgg ttaagccttc taatgaagca attaaaggca tgatcaatac
    31681 tgtttctcac ctagtggacg ttgaagaaat ttaatttaga aaaatcataa ctagaacatt
    31741 aaggaggtgc caaacctatg aaacttcatg aattaaaacc tgctgaaggt tcacgccaag
    31801 tacgtaaccg tgttggtcgt ggtacttcat ctggtaacgg aaaaactgcc ggacgcggac
    31861 aaaaaggaca aaaagctcgt tcaggcggcg gtgtgcgttt aggatttgaa gggggacaaa
    31921 caccattgtt ccgtcgttta ccaaaacgag gattcacaaa cgtgaaccgt aaagactatg
    31981 cagtcattaa tcttgatgta ttgaaccgct ttgaagacgg agctgaagta actcctgttg
    32041 ctttagttga agctggaatc gtgaaaaacg aaaaagctgg aatcaaagta ttagctaatg
    32101 gggaactaaa caagaaatta actgtgaaag cagcaaaatt ctctaaagca gctcaagaag
    32161 caattgaagc ggctggtgga tcaatcgagg tgatctaatg tttagactat taaaagatgc
    32221 ttttaaagta aaagacatta gatcgaaaat cctatttact gtatttgtgt tgtttgtatt
    32281 tcgcctaggt gcacatatta ctgtaccagg cgtaaatgct gcttcattgc aaaacctttc
    32341 ttctttgcca tttttcaaca tgttgaactt ggtaagtggt agtgcgatgc aaaacttttc
    32401 cattttctca atgggggttt caccatacat tactgcttct atcgtcattc agcttcttca
    32461 aatggacatc gtgcctcgat ttgttgaatg gtcgaaacaa ggggaagttg gtcgtaaaaa
    32521 actgaatcaa gcgactcgat atctgacatt agtattggca tttatccaat ctgtcggtat
    32581 cactgctgga ttcaatcaat acgctcaatt aggtttcgtg aataatccta acgtagcaac
    32641 ttacgtaacg atcggtttga ttctaacagc cggtacgatg tttgttactt ggctaggaga
    32701 acagatcaca gaaaaaggta tcggaaatgg cgtatcaatg atcatttttg caggtatcat
    32761 ttctagattg cctgaaagta tcagagaaat ttatgaagat tactttataa atattgatcc
    32821 ttcagacata tggaaatctg ctatttttgt ggccatttta ctagtagcgg tcttgttgat
    32881 cgtgacattc gtaacatttt tccaacaagc tgaacgcaag atcccgatcc aatatacgaa
    32941 acgcgtagct ggcgcgccta caagcagcta tctgccgttg aaagtaaatg cagctggagt
    33001 tattccagtt atctttgcta gttcgtttat tgctacgcca aatgctgttt tacaagcatt
    33061 gagcggtaga ttcggttctg aaaactggta caaagtgatg atggagatct ttaactataa
    33121 tacagcacct ggtgctacga tttatacgat tctgatcgtt gccttcactt tcttctacgc
    33181 ctttgttcaa gtaaaccctg agaaattagc ggaaaacttg caaaagcaag gaagttatat
    33241 accaagcgta cgccctggta aggggactga agagtatgtc tcaagattac tgatgcgttt
    33301 aagtacagtt ggtgcagtct tccttggttt agtggcattg cttccaatca ttgctcaaat
    33361 ggtttgggat cttccacaat caatcggatt aggcggtaca agcttactga tcgttatcgg
    33421 tgtagcttta gaaactgcga aacagttaga aggcttaatg ctgaaacgta aatatgttgg
    33481 attcattgag taagaaagcg tgttgaggtt tcccctcaac acacgttctc acacatttat
    33541 taggagggaa aacaatgaac ctcattttaa tggggctgcc aggcgcaggt aaaggaacac
    33601 aagctgaaaa aatcattgat gcttatggta ttccgcacat ctcaacagga gatatgttcc
    33661 gcgcagcgat gcaaaacgaa acagctcttg gcctggaagc aaagtcttat atggataaag
    33721 gtgcattagt tcctgatgaa gtaacaaatg gaatcgtgaa agagcgacta gctgaaccag
    33781 atacagagaa aggcttctta ttagatggct ttccacgtac gctagatcaa gcaaaagctc
    33841 tggacgcaat gctaaaagat ttgaataaaa aaattgatgc tgtcatcgat atccatgttg
    33901 gtgaagaaat cttggtcgaa cgtctagccg gccgatttat ctgcagcaat tgtggagcaa
    33961 cttaccataa aattttcaac cctacaaaag ttgaagacac atgtgatcgt tgcggtgggc
    34021 atgaattcta tcaaagagaa gatgataaac ctgagacggt taaaaatcgt ctggctatca
    34081 acatcaaaaa tagtgaacca atcttggctt attataaaga acaaggtttg ctaaacacta
    34141 ttgatggtgc tcgtgaaatc gatgctgtat ttgcagatgt acaaaaaatc attgaaaaat
    34201 agcacgattg cccgtagccg acttgcatta tgcagttact cttgtcaagt tcaataaatt
    34261 atgatagaat atgtcagtcc gacttctaga gaatcctcta gatgatccat tttgagaatt
    34321 ttgaggtggg aactgcagtt tccgccattt tatcgtgtga aaatgcgaaa caagtacaag
    34381 gaggtactgt gcgtggcaaa agaagatatg attgaagtcg aaggtacagt cgtcgaaact
    34441 ttgccgaatg caatgtttaa agttgaacta gaaaacggac accaagttct tgctactgtt
    34501 tcaggtaaaa ttcgtatgca ttacatccgt atcttacctg gtgacaaagt aacggttgaa
    34561 ttgtctccat acgatcttaa ccgtggccgt attacttatc gctttaaata attgtactcc
    34621 gtaaaactac aggaggtatt atcatgaaag taagaccatc agtaaaacca atgtgcgaac
    34681 attgtaaagt aattcgtcgt aaaggacgcg ttatggtgat ttgcccagca aatccaaaac
    34741 ataaacaacg tcaaggataa ttggaggtgc tataaatatg gctcgtattg caggagtaga
    34801 tattcctcgt gataaacgtg tagtagtttc acttacttat atctatggta ttggtaacac
    34861 tactgctaaa aagatcttag ctgatgctgg tgtatctgaa gatgttcgcg ttcgtgaatt
    34921 aacgaatgaa caaacagatg ctatccgtgc agaagtagat aaattaaaag ttgaagggga
    34981 tcttcgtcgt gaagtgaact tgaacatcaa acgtttgatg gaaatcggtt cttaccgtgg
    35041 aatccgtcat cgccgtggat tgcctgttcg tggacaaaac acgaaaaaca atgcacgtac
    35101 tcgtaaaggc ccagctcgcg ctatcgctgg caagaaaaaa taattcttaa gtgaaggagg
    35161 ttaaaacttc atggcagcaa aaaaagtgaa tcgtaaacgc cgtgtgaaga agaatataga
    35221 agcaggtatt gcacatatcc attctacatt caataacact atcatcatga tcactgatac
    35281 tcacggaaat gcattagcgt ggtcatcagc tggttcatta ggtttcaaag gaagcaaaaa
    35341 atcaactcct tttgccgctc aaatggcagc agaaactgct acaaaagcag caatggaaca
    35401 cggattaaag actgttgaag taacagttaa aggacctggt tctggacgtg aagcagcaat
    35461 tcgttcatta caagctacag gtttagaagt gactgcaatt cgtgacgtga ctccagttcc
    35521 tcataatggt gcccgccctc caaaacgccg tcgtgtttaa tgagtgccgc tcattttgag
    35581 ttttatcagt aacgtcattg aacaagacac ttttcgtttt gaaaggggta aagataagaa
    35641 tgattgagtt cgaaaaacca agaatcgcaa aaattgatga agaaaaagat tatggcaagt
    35701 tcatcgttga acctcttgaa agagggtatg ggactacttt agggaattcc ctacgtcgta
    35761 ttttattatc ttctttgcca ggtgctgcca tcactaatat tcaaatcgat ggtgtactgc
    35821 atgaattctc taccatcaaa ggtgttagag aagacgtcac tcaaatcatc ttgaacatta
    35881 aaggcttagc gctgaaaatg tatggccaag aagaaaaaac ccttgaaatc gatattaccg
    35941 gacctgctac agtaactgct ggcgatatta tcgttgatag tgatgtagaa attcttaaca
    36001 aagatatgta catctgtagt gtctctgaag gagctacgtt ccatgctcgc ttaacagtga
    36061 aacctggacg tggttatgtt caagcagatg aaaataaaaa agaagatatg ccaattggcg
    36121 ttcttccagt cgattcaatc tacacacctg ttcgtcgtgt gaactatcaa gttgagaaca
    36181 cacgtgttgg acaccgtgaa gattttgata aattgacgat ggagatttgg acagatggtt
    36241 caattgaacc gttggaagca atgagtttgt ctgcgaaaat catgactgaa catttggata
    36301 tttttgtgaa cttaactgat gaagcaaaaa atgctgaaat catggtcgaa aaagaagaaa
    36361 cacaaaaaga aaaaatgttg gaaatgacga tcgaagagtt agacttgtct gttcgctctt
    36421 acaattgttt gaaacgtgca ggaatcaata ctgtacaaga acttacaaat aaatctgagc
    36481 ctgaaatgat caaagtccgt aacttaggac gtaaatcatt agaagaagtt aaagcaaaat
    36541 tgcacgactt aggtttaggt ttacgtaaag aagattaaaa ccttttacga aggagggaaa
    36601 ccaacgtgag ttatcgtaaa ctaggacgca catctagcca acgtaaagcg atgttgcgtg
    36661 atttaacaac tgatttatta atcaacgaac gtattgtgac tactgaagct cgtgcgaaag
    36721 aagttcgctc aactgcagaa aaaatgatca ctttaggtaa acgcggagat ttgcatgcac
    36781 gtcgtcaagc tgcggctttc gtacgtaatg aagttgccag cgtgcgtgaa gaaaatgagg
    36841 acatcgtcat tgaatcagct ttacaaaaac tgttcaacga tattgcacct cgttacgcag
    36901 aacgtcaagg cggatacaca cgcattttga agacagaacc aagacgcggt gatggtgcac
    36961 caatggttgt cttagaactt gtctaaaatc acactaataa catcacttta tagatgattc
    37021 ttttagagtg ttatgatgtt ggaagatatt tttccgagtc tagctcagcg ctgctcctca
    37081 agcgaatatc gcttggggag taagcactca tcataaagtg tttttttata ccttcaatta
    37141 cctgtcatga cagatatgtt tgaaggcata aaaaaacatt ttaagcgaga aataattgaa
    37201 aattttgtct ttttttgata gtgttagtag gaactttagg atgaagaagg agtaatcatg
    37261 gagccaatta ttgaattaga aaagataaat tataaatacc agccagatga tcttcgcccc
    37321 gcattgaaag atgtctcctt tacaattgat aaaggtgagt ggatcgctat tattggacac
    37381 aatggttcag gaaaatcgac acttgccaaa acgatcaatg gattgcttct gccagaatca
    37441 gggatcgtga aagtgggaaa tcaaatattg gatgaagaaa atatttggac gatccgacag
    37501 atggttggaa tgatttttca aaatccagac aatcagtttg ttggttcgac agtagaagat
    37561 gacgttgctt ttggtcttga aaatcaaggg attcccagag aagaaatgct agtgcgtgtg
    37621 aaagatgcac ttgagaaagt tagaatggct gagtttgctt cacgcgagcc tgctcgttta
    37681 tcaggagggc aaaaacaacg tgttgccatt gccggcgtgg ttgccttacg acctgatatc
    37741 attattttag atgaagcaac cagtatgcta gatccagaag gtcgtgaaga agtgatctct
    37801 acgatcaaga agatcaaaga agaaagtcaa ttgaccgtta tatctatcac tcatgatatt
    37861 gatgaagcag ctaatgccaa tcggatacta gtgatgagac aaggcgaact tgtccgtgaa
    37921 ggtacaccaa aagaaatttt ttctgctggt cctgaactga tcgacttagg tctagatcta
    37981 ccatttcctg aaaaactgaa gagcgcactg aaagaacgtg gggttgatgt gcccagtgaa
    38041 tatatgacag aagaaaggat ggtggattgg ttatggacat ccgttttgaa caagtagact
    38101 tcacttatca gccgaataca ccatttgaac aaagagcctt atttgacatc aatatgacga
    38161 tcaaagaaaa cagttatact gcattagtag ggcataccgg aagcgggaaa tcaaccttac
    38221 ttcagcattt gaatgcgctt gttaagccaa caagcggtac agtccatatt ggagaacgag
    38281 atattcagcc ggatacggat aataagaatt taaagcccat ccgaaaaaaa gttggcatcg
    38341 tctttcagtt tccagaagca cagctattcg aagaaacggt agcaaaagac attgctttcg
    38401 gtcctaaaaa ctttggtgtc agcgaagaag aagcactagt cctagcaaaa gaaacattag
    38461 aacaagttgg gctggatgaa agctatttgg aacgttcgcc atttgaactt tcaggagggc
    38521 aaatgcgtag agtagcaatc gctggtgtgc ttgccatgag gccggaagta cttgtattgg
    38581 atgaaccaac ggcaggactt gatccccagg gcagaaaaga aatgatggag atgttttggc
    38641 gtcttcataa ggaacagcag atcactattg tattagttac gcatttaatg gatgatgtag
    38701 ccaattttgc agattatgtc tatgtgttgg aaaagggacg aatcgttaat agcggggagc
    38761 cgcaagaggt ctttcaaaac attgaatggc taaaagaaaa acaactaggc gtacctactg
    38821 ccaccgagtt tgcagaagaa ttaatggcta aagggatgaa ctttgctacc ttgcctttga
    38881 cagctgaaga attagcagac gctatcgtcc attatgctgg aggaaatgcc catgatgaat
    38941 aaattgatat ttggtcgtta tatgcctgga gattccttga tccatcgatt ggatccccga
    39001 gctaaattat tagccagttt ttattttatc ggaatcattt ttttagcaaa taattgggaa
    39061 acgtaccttt tgttggcatt tttcacatta ctttctgtct tcttgtcaaa agtagatttg
    39121 aaatttttta tccgaggcat tcgcccattg atctggctaa tcctcttcac tgtagctttg
    39181 caggtcttgt ttacaagtgg cggagaaact tattggagtt ggggtgtttt ccatatcact
    39241 gaatttggtg tgatgaacgg cctctttatt ttctgtcgat tcgtgttgat catttttatg
    39301 tcgactctac tcacgcttac tacaccgcct ttggaattat cggatgcgat tgagtacttg
    39361 cttcgtccat tgaaagccgt acggttccca gtccacgaaa tttcattgat gctgtcgatt
    39421 gctcttcgtt ttgtgcctac attgatggac gaaacagaaa agatcatgaa cgctcagcgc
    39481 gctcgcggtg ttgattttgg tgaaggaaac ctagttcaga aaatgaaagc agtcattcct
    39541 ttgctgattc ctttatttgt cagcagcttc aaccgtgcgg aagatcttgc gactgctatg
    39601 gaagcaagag gctacaaagg tggggaagga agaacgaaat atcgtgtatt gcattggcat
    39661 aatagagaca cgatggttat cattgctttt gtattattaa ctgtgctact gatcttttta
    39721 agaggataaa cgttcagtgg gccgctatct attcttttct tgctgatctg tttgcaagaa
    39781 aggatgcaag cagtgtatga caagaaatat tagggtctgt ggcagactaa ggtcacaggc
    39841 ctctttcttt tatctgaaaa gaaagaacga aaaaatttta tagaaaaatc attattccag
    39901 ataacctttt aggaacaaag gagagaaaaa catggtacgt tacaaagcaa tcattgcgta
    39961 cgacggaaca aattttaatg gattccaaaa acagccaaat gggcgtactg ttcaagaaga
    40021 agtagaaaaa acattacaaa aaatggcaaa tggaaaagaa atcacggtct ttggttcggg
    40081 aaggacagat gccggagtac atgcgatcgg gcaagtaatc cattttgatt atccagagga
    40141 gcgtccgtta gaacgaatgc gtttcgcgct agatacccag tcaccagagg atattgctgt
    40201 aagacaagta gaaattgttt cagaagactt ccacgcaaga tatttggtca aagaaaagac
    40261 gtatcaattt cgagtggata tagggaaacc acgcagtcct tttcgacgcc attatgcaag
    40321 ttattttcct tacccacttg atttggaaaa aattcagcgg gcgcttcctg atttattagg
    40381 gactcacgat ttcacctctt tttgtgcatc aggaagctcg atagaggata aaatacgtac
    40441 gatctacgag gctaagatgg aggtaaatga aacaaaagac gaattgttgt ttacatttag
    40501 gggaaatggc ttcttgtata agatgatacg gatcttagta ggaacgcttt tgaaaattgg
    40561 caatggccga ttgcctgaag atagtatccc agatattatt gcgaaaaaag atcgtaatgc
    40621 agcaggacct acagcacacc ctgaaggact ttatttgtat gaagtgaaat atgaataaga
    40681 ggttatgata gaagtggtca gcttcaagaa ataagaaggt atttacgaaa attacttctc
    40741 aaaattttgc aaaatttcgg cttattttcg aagaagctac ttctggaaca ccgtttaaga
    40801 aagaggtcgt gagcctgtca tttaactctg agtcacaaag agcaatttca aaaaacagct
    40861 tctcatcttt gatgaatatc gtgacttgtg gacgaggcgt tgataagtga atcatctttt
    40921 tgttacctat tttttctgta accaaaaaac gatggaacgt tgttttgatt gcccttggtg
    40981 cggacagtct gaacaggttc catcgtttct ttattttcta cgtcttcttc tgtgtgtgtt
    41041 tcgtggttgt tcttctattg ctggatcaat cgtgccataa aaggggcgat tgcggtcaag
    41101 gtaccagaca ttaagaataa acaatgcaat acataggaca aaaaggattg cacctatcgt
    41161 aaatccttca ggcaagtaac tgaaagtgac agtatgttta cctgctggaa cttttatcat
    41221 caagaatgcg tctttgaaat tgctgacctt gactggtttc ccatcaattt tagctttcca
    41281 tcctttgtca taaggaatag ttgtcaccaa catggaatct tcagcggtat ttacagtccc
    41341 agaagctttt ctgccagtgg cttccatttc gacacctttt tctttgatct gattcattgc
    41401 ggcttgatag gctacaacat ctagaccaac gactttaggc tccatgaaac ttatttcttt
    41461 cgtcccataa aaacttgcag taaatgtcac tgttgtctct tgaggataat agccaagatc
    41521 atagtattga ccagaaatgt tgatttgcgt ttttcgcgat gtaccattga cagtgatcgt
    41581 cgcactagaa ctttctaatt gcgcaaaatt ggttgggaac aaacttaagt aagcttgtgt
    41641 atttgcaggc acatggactt cccaagtgag ctcttttggc tggttatctg ctgtttctga
    41701 gtaagtgacg ccagtcgttg tctttgtgat cgtcgtattc aactgattct tcaaagtagg
    41761 tgtatagaat gtaaaatact gttcatttag accagacaat gtattgatca agtttgtctg
    41821 actggtcaaa ttatctgtca ctggctgctc aacatcatta acggacaacg gtgcagtaaa
    41881 tgctagtggc aatgcattga cattctgata cagtgtatag tcgccgcttt gaccagcttg
    41941 gcggaagcca tatttactga acgtgctttc gtttttagaa atgttatatt tgattcctgt
    42001 aaacccatcc atcagcaaag tattattgtt ataacggata ttcaaatttg tccctcttga
    42061 acggaaacct aatttatcca ggtaagagga agaattacga tttcttatag aagaaaacaa
    42121 actgatacca ctgtaaccat agttgatcga atcgtttgca gatactggat ccagattttc
    42181 caagcggtaa aacgagtcgg aatctttttt tgtttcattt acaagagctt tcaagtcagg
    42241 ataaggatca gtatataaac tgcgggaagc ataattccaa tcatcaagaa tgccatggat
    42301 catactgttc gtattgacca gtgcttcagc agacatgatc aataataaca atatactcag
    42361 atgattgaat gaaagttttt tatgctgata aaatcccaca gcaaaagcat aaatccctaa
    42421 gaaaaccact gtcaatacaa aggaagtcat cgtcacatag gtgtagcttc cttcaggttt
    42481 gatgccccat gccaccgcaa agagagcagc tacgaccaaa atgattccta acaacagccc
    42541 accatcgtct tgttctatct tttcccatcc ttttcctgca agcaggagca ctaagaaaga
    42601 gaacagatag gcataacgga ataaaaacat attcggtgca tgcataccat gccagaacaa
    42661 attcaatgga acaagataaa aacttgcgat aagtatggcg aaaagactgc cgtacaaaaa
    42721 tttatttttc aacggtactt tccgactggc aaaataaaac agacagagga ctaatggtag
    42781 cagacctgcg taaacgaacg gaatcgatcc gtattttgtc gtgtcataaa caccaatcat
    42841 atttttcatg attagatcca gataagaagt cgcttctgtt ttcaacgtag tgatctgact
    42901 cagtgcctcg ccatttgacc gaagatccag cactgctggc aatacaatga tcattgaagc
    42961 gccgcctgcc agtaaagaag tgataccata aggaacaatc gtttttttat attctgacca
    43021 gtttgtcaat agacgggcga taaaatataa gaaagaaaat acgccaatca taaaacccat
    43081 ataaaaactg gataaaaaca agagtaaata actgataaat aaaagtttcg gccgtttaag
    43141 atccataaca cgatgaatcc ccaaaatcac taatggaaga taaacaaatg catccagcca
    43201 catgatgatc tccgaatgtg cagtgataaa tgagatcaat gcgtaactga cgctaagtgc
    43261 aacatgcccc cagcgaggaa tcttgaacgt acgtgcaaga aaccaaaaag ccaatccggc
    43321 acagccgacc ttaagcaaag tcagaacata cagtgcatct ggcatcatct ggtttggaaa
    43381 aaagaacacc agtggcgtga agatcccgcc tagatagtaa gaaattaaag ataaataatt
    43441 caatcctaga gaagcattcc atgtataaaa aatactttgc tttccaagaa gaacatttcg
    43501 gaagctggca tgaaaattag aaaactgtga aaaggcatca cttgccaaaa tacttctgct
    43561 gcttccagga tagattccga tacttagata aacaagtgct aaaatcaaaa acggaatcag
    43621 gaaactagct gctgcgtagc gtccgttttc tctaaagaat gactttaaat tttttttcat
    43681 atttattttc caccaaattt ctatgaagtt gtattcagtt tatcatatcc ttgtttttct
    43741 ctgcttaaaa agagtctagt tttcacaaaa aaataaaatt tagaataaat aacttcataa
    43801 tttgttcgta taacaaaaat aaacgcaaaa aaagggtagg aatcaaaagt cccaccctta
    43861 aaaattttta ttaaataaaa aaccgttcac tatctgaacg gtccaagtat cattcaatta
    43921 aacgcgttca acttttcctg atttcaaagc acgagttgac acccaaactt ttttaggttt
    43981 tccgtcgacc ataacgcgaa ctttttgtaa gttaggttta acagtacgtt ttgttgagtt
    44041 catagcgtgt gaacggttgt ttccactttt tgtcttgcga cctgtaaagt aacaaacttt
    44101 tgccatggta atttcctcct ttgccttgat ttggagcaat agaataattc agctcatacc
    44161 agactaacat atcatataga atgggaattt gcaagtacat tttcatagat acaattgaca
    44221 attgagaaga ataattgtaa attaaacaag gttatacctg aaattttgca aggtttttga
    44281 gtactttggg taactgcctt ttatttcaag ttgcgctatg ataaaatatg atagaagaaa
    44341 aatgggagtt ttttacccgt taaggaggct cattcatggc tgtaaaaatt caaacgtcag
    44401 ctggaaccat tgaaattacc aatgatgtaa ttgcaacagt tgttggtggc gccgcgacag
    44461 atgtgttcgg tgttgtagga atggccagca aaaatcaaat taaagataat attactgaga
    44521 ttttgcgtaa agaaaattac tcaagaggag tagttgtccg ccaagaagat aatggtattg
    44581 ctgtagatgt ttatacgatt atcagctatg gtacgaaaat ctctgaagtt tcccgcaacg
    44641 tccaagaaaa agtgaaatac aatctagagg cattattagg cgtaactgcc aattcagtta
    44701 atgtctttgt acaaggtgtt cgtgtactgc cagactaggc aggagactgg ctggtaactc
    44761 agcagtcgat ctgaaggagg attatctagg tgagtgtaac agaaataagt gcaagccagt
    44821 tccaggaaat ggtccaggca ggtgcgaatc gtctgcaagt aaatgcagaa tacgtcaatt
    44881 cattaaatgt attccctgtg ccagatggcg atacgggaac aaatatgaat ctatctatga
    44941 ctagtggagc taaggcagta gtagattcaa cctcagaaaa agtaggagaa ttagcagcaa
    45001 tgctttcaaa aggattgctg atgggtgcgc gcggaaattc cggcgttatt ttgtctcaat
    45061 tgtttcgtgg attttctaag caaattccag atgttgtctc attaaatgct tctgatcttg
    45121 ctgcagcctt cactcatgga gttgaaactg cgtataaagc agtaatgaaa ccagtagaag
    45181 gaacgatttt gacagtagct agagaagcag cgaaagctgg agaaaagaaa gcaaaaagta
    45241 ctgatgatgt cgttgaagta atgacagctg ttgtaaaagg agcaaagcgt gctttagcta
    45301 aaactccaga cttgcttcca gtcctaaaag aagtcggtgt cgtagacagt ggagggcaag
    45361 gactgctgtt tgtttatgag ggctttttaa gtgcattgaa tggagaattc caagcggatg
    45421 aaacatatga accttctcca gcagaaatgg atgatatggt caatgcagaa catcatcgca
    45481 gtgtccaagg tcaaatggcg acagaagata tccattttgg ttactgtaca gaaatcatgg
    45541 taaaaatcgg agaaggacct acagtcgata gtacattcga ttacgaagaa ttccgtaact
    45601 atttagatgg tataggagat tctttacttg tggtcaatga tgacgagatc atcaaagtcc
    45661 acgtgcatac agaaaaccca ggagaagtct tgaattatgg acaaaaattc ggttcattag
    45721 tcaaaatcaa agtcgacaac atgcgcttgc agcatgagac gattttggaa cacgatgaag
    45781 aagtgaacga gtttgcgaaa aaagaaatac cacgtatccc gtatggtatc gtagcgatcg
    45841 ctgctggtaa aggcgtccaa gagctgtttg aaagtttagg cgctaattat gttatcagtg
    45901 gtggacaaac aatgaatcca agtacggaag acattgttaa agcgattcaa gaagtaaacg
    45961 cagataaagt gattgtcttg ccaaacaata agaacatctt catggcagct gatcaagcag
    46021 cagaagtagc agatatccca gtagcagttg ttccttcaag aacgatttcc caaggattga
    46081 cagctatgct ttcattcaat gatcaagctt ctttagaaga aaacaaagaa acgatgacag
    46141 aaatgttgtc tagcgttgtg agcggtcaag tcactcacgc gatcagagat acctcaattg
    46201 acggtgtaga gatcaaagaa ggagactttt tgggcatgat cgatggaaaa atcctgatct
    46261 ctaacccaga tatcacagca acagcgttaa gtacactaga acaaatgatc aatgaggaca
    46321 cagagatcgt aacgatcttg gcaggtgatg aaggaacagt tgaacaagca aatgaatttg
    46381 ctgagaaact aacagaaaaa catcctgatt tggaaatcga gatccaccaa ggagatcagc
    46441 ctgtctatcc ttatctattt tctgctgaat aatttctgtg tcagaatatc tttctaacac
    46501 agtattcatt cataaaaggc cttcggttag tgatcaggta tgatcgctga ccaagtcctt
    46561 tttttgttaa aggagggagg aaaaatttat gcgttcttta gatgatccaa tcactatgct
    46621 ggcaggagtc ggagaaaaaa gagcagctag tcttgccagc ttgggcatcc aaacgattga
    46681 agacttactg acttattatc cttttcgcta cgaagatatt caggaaagaa atctaaacga
    46741 gatccaggat caagaaaagg ttactttgaa aggaatcgta gtctcgcctc ctgtgatgaa
    46801 tcgatttggt tataaaaaaa gccgattgca atttcgtatg atgcaagaac atgatgtgtt
    46861 caacgtttct ttttttaatc aaccttatct aaaagaaaaa gtcattttat cggaggaaat
    46921 cgccgtatac ggtaaatggg atgcaaagcg taaagccttg aacgggatga agattttagg
    46981 ttctcaaaca atggatgact tttcgccgat atatcacgtc aacaaatcaa ttcgtcaaac
    47041 gacattagtt gatctaatca aaaaagggtt tcaagaatac ggcacattga ttgaagaaaa
    47101 tctgccaaac aaccttgtgg agaaatatcg actgcttgac cgagcttctg ctgtaagaag
    47161 tatgcatttt ccaaaagacc acgaagaaaa ccatcaagcc aaacgacgtg tcgtttttga
    47221 agagttcttt ttatttcaaa tgcgtcttca aggattgaaa aaagcagaaa aagcagaaac
    47281 gaatggtttg gagatccttt atgatgtcca aaaattgaaa caatttactc aaaagctacc
    47341 ttttgaactg acaaatgcac aaaaacgtgt aacgaatgaa atctgtcggg atcttcgcag
    47401 tccgcagcat atgcagcgac ttttgcaggg agatgtaggt agcggaaaaa cagtggttgc
    47461 cgcgattgcg ttatacgcta ccgttacagc aggtttccaa ggtgcgttga tggtgcctac
    47521 agaaatcttg gcacaacaac atatggaaag cttgcaacag ctgtttgatc ccaatgaagt
    47581 aaccatcgca ttgattacgg ggtcaacgaa agcaaaagaa cgacgtgagc tccttgaaaa
    47641 actagagcaa ggggaaatta acgtcgtgat cggcacacat gcattgatcc aagatggagt
    47701 cgaatttcag aatttaggtc tagttattac ggatgagcag caccgttttg gcgtgaacca
    47761 gcggaaagta cttcgtgaaa aaggctggcg accagatgtc ttatttatga cagcgactcc
    47821 gattcctaga acattagcta ttacagcata tggtgagatg gatgtatcta tcattgatga
    47881 acttccagct ggacgtatcc cgatcgaaac acgctgggta agaacacctc aattggacag
    47941 tgtactggat tggacgaaaa aagaactggc aagaggacat caaatgtatg tcatctgtcc
    48001 gctgattgaa gaatcagaag cattagatgt aaaaaatgca gtggaaatct atgaaaaact
    48061 tcgtgaacta tttgcaccaa aatatgaagt tggcttgtta catggtaaaa tgaagaatca
    48121 ggaaaaagaa gtaatcatgg aagccttcaa agacaaccag atgcagatat tagtttcgac
    48181 tactgtaatt gaagtagggg taaacgtgcc gaacgcgacc gtcatgctga tcatggatgc
    48241 ggatcgattt ggattggcac agctgcacca acttcgtgga cgtgttggac gcggaagtga
    48301 tgcttcctac tgtatcttag ttgctaatcc taaaaatgaa ctaggtgtgg aacggatgaa
    48361 aatcatgaca gagaccacca atggttttgt actaagcgaa aaagatctgg aattacgtgg
    48421 tcctggtgaa gtatttggct tccgtcaatc tggtctgcct caatttgcga ttgctgactt
    48481 ggtgacggat ggaaatgttt tggaggtagc cagagaagag gctgcagccc tttggcaaat
    48541 aaaggattgg cagatgcttc ctgagtatca gcctttagcg caaagcctcc aaatagaaaa
    48601 tcaggataat cgttatttcg attagcagac tgtgctatac tttatactga tgacggaaaa
    48661 aggagtggaa acaatgaaaa ttgccgtaga tgcaatgggc ggagatcacg cacctcaagc
    48721 aattgtagaa ggcgtggctt tagcgcagca agattttcca gaagtagaat tcttacttta
    48781 cggaaaagaa gcagaaatca agaaatactt aacaaatgaa aaaaacatca cgattatcca
    48841 tacagacgaa aaaatcaata gtgatgatga gccagtaaaa gcaattcgac gaaaaaagac
    48901 tgcatcaatg gtccttgctg cacaagcagt gaaaaacggt gaagcagatg ctgtattttc
    48961 agcagggaat acaggcgcgc tactagcagc agggttgttc atcgtaggac gtatcaaaaa
    49021 tatcgaacgc cctggcttga tgtctacctt gccagtagta gggaaagaag gtgccggttt
    49081 cgatatgctg gatcttggcg ctaacgcaga aaacaaacca gaacatttgt tgcaatacgg
    49141 tattttaggt tctttttatg cacgtaaagt ccgaggtatc ggacgtcctc gtgtcgcact
    49201 cttaaacaac ggaacagaag caacaaaagg tagtgaagtg acgaaaaaag cctatgaact
    49261 ccttcagaat gaaacaagct tgaattttat tggaaacgta gaagctcgtg agctactcaa
    49321 tggtgtggct gatgtagtgg tcacagatgg atttacaggc aacgctgttt tgaaatctat
    49381 tgaaggaaca gcaatgaata tgatgaatct attaaaatct gctattctaa atgaagggat
    49441 caaaggaaaa atgggcgcta tgcttttaaa agacggctta cgttctttga aagcagaaat
    49501 ggattattcc aaacatggcg gtgccgtttt gtttggcttg aaagcaccag tcattaaaac
    49561 tcatggagca acaggccccg atgctgttcg ctatacgatc cgccaaatcc acacaatgct
    49621 tgagacagat gtagttggac aattagtcga acaatttgaa aaaagcgaag aatagtattt
    49681 ggatatctga agcgatccgt tccattgaaa aaaaggtgga cattcctgaa aaatgccagt
    49741 aaaattagcc atggtgtagg taattgacaa gtgtatgatt cttggaggtg aaaaagttga
    49801 ctcgcgaaga agtatttaac aaagtagcga aaattatttc aaatcatttt gagatagata
    49861 ccgataaagt gacggatgaa ttgaacatca aagatgattt aaaagctgat tcaattagta
    49921 ttatggaatt cgttttagag cttgaagatg aattcggtac ggaaatttca gatgaagacg
    49981 ctgaacaaat tgaaacagtc ggtggcgcag tagattatat cagcagccat cttaaataat
    50041 ttcaaataaa agcaaagaag gataaaaagc tgcatgcagc tttttatcct tcttttttta
    50101 atctttttga cacgtaaaag tcatttttct gataattctg gcgataaaca gaaattatgt
    50161 tgcaaagtaa agcggttttc tatataatta ttaataattc attacacaaa gataagaata
    50221 acctttgtta taaaacgatt tattctgtgt aatgtccttt tttattaaga aaaacttcca
    50281 tttaggaagc agagaggggt attcgattgt ccgataacca attattagaa gttcagaacc
    50341 tacatacagg attccgactc aaagatgact attacgatgc agtagatgac gtttcattta
    50401 ctttagacaa aaacgagatt ttagcaatcg ttggggaatc tgggtgcggg aaaagtacct
    50461 tggctactac gattatggga ttacttgatc ctaataacac aaaaattaca ggtgagattt
    50521 tatataacga tctgaattta atcgacctga acgaaacgct ctataataaa atccgcggaa
    50581 atgatatcgg aatgattttc caagatccgt tgtctgcatt aaatcctctc atgagaatcg
    50641 aagaccagat caaagaagga ctttcctatc acacaaagat gaatgcagaa cagcgtcaag
    50701 ctcgtgcact agaattgcta gaacaagtag ggattcctaa tcctgcgaga gttggacgcc
    50761 aatatcccca tgaactatca ggcggtatga gacaacgtgt catcatcgca atcgcaatcg
    50821 cctgcaaacc tccaatcgtc attgcagatg aaccgacgac agccctagat gtaaccatcc
    50881 aagcacagat tcttgattta ctgaaagacc ttcaggaaga aacaaaaaca gggatcatct
    50941 tgatcactca tgacttagga gtcgtagcag aaatggctga tcgagtggca gtgatgtatg
    51001 gcggacagtt cgttgaagta gcaggtgtaa aagaactgtt tgatcatcct aagcatcctt
    51061 atacacgctc tttactgaac tctataccac aagaaggaaa tcatgatgca gaactccatg
    51121 tgatcgaagg tgtcgttcct tctctgaaaa acatgcctcg taaaggttgt cggtttgcac
    51181 cacgtattcc atggatacca gcaagcgatc atgaagaaga gcctatcctt catgaagtcg
    51241 caccgaacca cttggtacgt tgctcctgct ataagcattt ccactttaga gacgaaaaag
    51301 gagaggtgta gatgacagaa ttaatcacaa tcaaagacct gaaagttcat taccctattc
    51361 gcagtggatt cttcaatcga gtaacggatc atgtatatgc tgtagatggt gtggatttta
    51421 ttatcgaaaa aggaaagaca tatggtttag tgggagagtc tggctctgga aaatctacta
    51481 cgggaaaagc cgttgtggga ttagaaaagg taacagccgg acaaatcatg tatgaaggaa
    51541 aagatgtaac gaagcgaagc aaccgcaaaa aaatgggtta taacaaggat gtgcagatga
    51601 tcttccaaga ttcaatgtcc agtttgaatc ctaaaaagcg agtgttggat atcattgctg
    51661 aacccatccg aaattttgaa cggttaagcg atcaagaaga aaagaaaaag gtaaaaggac
    51721 tgctagatat cgttgggatg ccggaagatg cgctatataa atatcctcat gaattttcag
    51781 gcggacaacg tcaacgtcta ggagtagccc gtgctgttgc taccaatcca aaactgatcg
    51841 tagccgatga acctgtatct gctctggatc tatctgttca agcgcaagta ttgaatttca
    51901 tgaaacgaat ccagcaagaa tacggcctca gctatttatt catttcacat gatttaggtg
    51961 tagtaaaaca catgtgcgac aatatcgcaa ttatgtacaa aggtcgattt gtagaaatcg
    52021 gtacaagaga agacatttat aatgacccgc gacatatcta tacgaaacgt ctgctttcag
    52081 ctattccgca aatcgatgta gaaaataggg aaagccacaa agaaaatcga cgaagagtag
    52141 aacaagagta tatccaaaac caaaaagaat actatgatgc gactggtcga gtctacgatt
    52201 tgcgtacgat cacaactaca cacaaagtgg cgttgaaaga tggaggtgca agctaatgtg
    52261 gaaaacgatt ctgcgacgtg ttttattgat gatcccgcaa gttattattt taagtgttct
    52321 gatcttctta ttagcccaag cgatgccggg ggatccattt accggtttga tcaatccaaa
    52381 tcaggatccg gcagtgatcg aacagatgcg acaagcagct ggcttgaatg acccatggta
    52441 tgtccagtac ttccgttgga tcggaaatgc tttgcaaggc gattttggtc aaagtttctt
    52501 atacaaacgc tctgttgctg acttgatcgg cgaacggatc atcaacactt tatatctatc
    52561 gatcttgaca gttgtcctta cttatttgat tgctgtgcct ctggggatgc ttgctggccg
    52621 gtatcaaaat tcaatcttgg ataagattgt cgtaatctac aacttcttta gttttgctgt
    52681 tcctttattt atcttcggct tgatcatgct atttgttttt ggttatcgtt taggctggtt
    52741 cccaacaagt ggatcacaaa cactaggttt gcaaggtgga tttatcactc aatggataga
    52801 ccgtttgaaa tatattctgt taccatcaat tacccaagca tttttggcga ctgccgtcac
    52861 aatccaatat ctgcgaagtg aagtaatcga ttcgaaatcg ctcgattttg tcagaacagc
    52921 ccgttccaaa ggggtaccga cgaataaagt attcagtcga catattttcc gaaatgcagc
    52981 gttgccgatg gcttctcaaa tggggtatga aatcacttcg ttgattgctg gttctgtagt
    53041 tatcgaaaag atttttggtt atccgggagt cggaaaacta tttattgatt ccatcggcca
    53101 acgggactat accgtgatca catccttagt attgatttta ggaatcgcga cgctagtggg
    53161 gacattactt tcagatatca tcatgagtat cgttgacccg cgtattcgga ttcaataaaa
    53221 ccaatcagaa aggagaatgg atcgatgagt gaaaaaaaca atacagcagt tcaagaaagt
    53281 attcctccta tggggatacg aatgattgct cgagaattta aaaaagataa aattgctatt
    53341 ttttcattag gtttgctagt ggttttatta ttagtgatct ttatcggtgc gatgctgacc
    53401 gatcaagaca aagtgatgta tgttagtatc ttcgacaaat acgctgcacc aggaacagtt
    53461 tcgacacaag gacaaaagtt cattctggga gcagatgaag gcggacgtga tgtattagga
    53521 caattgatca taggtgctag aaactctgtt acgatcggat ttgcgatcac gatccttact
    53581 tcgatcatcg gggttggatt agggatcatc tctggctact acggagggat cattgacaac
    53641 atcttgatgc ggatcgttga ttttatcatg attctaccga tcatgctgat cattatcgtc
    53701 ttcgtatcga ttatttccag atacaatgtc tggtccttta tattcatcat gagtgcattt
    53761 tattgggtag ctaaagcccg attattccga agcaagacat tatctgaagc aagaagagat
    53821 tatgtcagtg cctcaaaaac aatgggaacc aatgatttta aaatcatgtt ccgcgaaatc
    53881 atgccgaatc taagttcatt aatcattact aatttgacga tgaactttgc ggcaaacatc
    53941 ggtatcgaga caactttgac tttcttaggt ttcggcttgc cggcaaatgt acctagttta
    54001 ggtacattga tcggttatgc aagtaacgga gaagtcttag tgaatcggca atggatctgg
    54061 ttacccgcat caatccttat tttagttttg atgttgagta tcaattatgt tggacaagca
    54121 ttcaagcgtt ctgcagatgc gcgccaacgg ttaggctagt atagaagcga agggggaaat
    54181 aggaatgaag aaaacgattt ttggatttgc agcgctgatg tctgctgtcg ttcttgcagc
    54241 atgcgggggc ggaggtacag atacctctgg aaacaactct ggaggcaacg gcaataacaa
    54301 agaggaatta acttttccaa ttgcaacaaa aaataatgat gatgcaatcg aagacggcga
    54361 attagaagct gcggtcgcaa cggacagtca gtttaaagga ttgttccagt gggagtttta
    54421 ccaagattca tatgatgcag cttttatggc tccttctcat gaaccgctat ttgcaaacga
    54481 tgacgatttc agaatcacag acgacggagc tgcaacgctt gatctagatg aagatacgaa
    54541 aaaagcgaca atcaaagtaa aagacaacgt caaatggtca gatggtgaag atgtaacggc
    54601 ggacgatgtg attttccctt acgaaatcat tgggaataaa gattacactg gtatccgtta
    54661 tgacgacact ttccgtaaca tcgtaggtat ggaagaatat catgatggaa gtgccgacac
    54721 aatctccgga atcaaaaaaa tagacgacaa aacagttgaa atcgaatata aagaagtaaa
    54781 cccaagcatg cttcaatcgg gtggtggtat ctggtcatat gcagcaccaa aacatacgtt
    54841 agaaggtgta gcaatcaaag atatggaatc aagcgatcaa gtacgtaaaa atccagtgac
    54901 ttttggccct tattacatga gtaatatcgt tgcaggagaa tcagtagaat acttgccaaa
    54961 cgagcactac tggaatggta cgcctaaatt gaaaaagatc acgatgaaat ctgtaccgat
    55021 ggcaagtgca actgaagcat tgaactctaa attatatgat atgattttcc aaatgccaac
    55081 agatacttac gatacctata aagaaattgc tggctataca aacttaggcc gtgaacaaac
    55141 ttcttatact tatctaggat tcaagttagg taaatggaat gcagatacaa agagtgtgga
    55201 atacaatcca gattcaaaaa tggctgataa atcattacgt caagcaatgg gctatgcatt
    55261 agataataat gcagtaggtg aacgtttcta tgcaggtttg agaagcaatg cgacttcatt
    55321 gattcctcca gtattccagt cataccatga tgatgagatg aaaggattca cacaagatct
    55381 agacaaagcg aaaaaactat tagatgatgc tggctacaaa gatgtggatg acgatggttt
    55441 aagagaagac aaagatggaa ataaactaac aatcaacttt gcttcgatgt ccggtgggga
    55501 aacagcgcaa ccattggcag attactatat ccaacaatgg aaagaaattg gtttggatgt
    55561 ccaatacaca acaggtcgtc tgatcgaatt caattctttc tatgataaat tagaaaacga
    55621 tgatcctgaa attgatgttt accaagctgc ttggaataca ggaactgatc caaatccaag
    55681 tggactatgg ggaccaaatg ctgcgttcaa ctacactcgt tatgaatcag aagaaaatac
    55741 aaaattgatc aacgatatca gttcaagcaa agcatttgat gaagagtacc aaaaagaagc
    55801 atatcaaaaa tggcaacaat atgcatttga agaagcattt gcgattccaa cgttgttccg
    55861 taatgaagta ttgccagtca acgaccgtgt caaagactgg gattggtctt atgatgtacc
    55921 aaacccatgg gcaaatgttt ctgttacagc agattcacgt tcataatcac taaaaatgac
    55981 cgaaaaaggg cgatgtcctt tttcggtttt ttgatatttt tctgtttatt tctctaatat
    56041 tcaggtcaag gcgctgattt catgacttct ccaataaacg gtgttcaaag tccagtttct
    56101 cggtattaag tcaaatttat gcaaaaatat ggaaagccat tttcacaaaa ttttttcttc
    56161 atactcgaaa ctaaacgaat ttttcacaac ttctcgtcaa tcactaggat tattaaagct
    56221 ttacttacct tttgaccgaa ccatttcaaa tttgacaaaa aaccagtata ataaggaagt
    56281 atcgaaaaaa tatccataaa aggaatgtgg aaaatggaca accagctgac aaaagaacta
    56341 aaagaaaact ataacatcgt ttttcacgat ctaaatttgc ttgaacaagc ttttacccat
    56401 tcatcctatg tgaatgagca tcgcaatctg caattatcgg ataatgagcg acttgaattt
    56461 ttaggagatg ccgttttaga actgatggtt tctcagtatt tattccgttt atatcctcat
    56521 ttgccagaag gaaaattgac aaaaacacga gctgcgatcg ttcgcgaaga cagcttgtcg
    56581 aaatttgcca aagaatgtca ctttgatcaa tatgttttgt tgggcaaggg agaagagaat
    56641 tcaggaggac gtacgcgacc agcattgctt tgcgacctat tcgaagcttt tttaggcgcg
    56701 ttatatcttg accagggatt cgacacgacc catgcattca tcgaaaaagt gatttttcct
    56761 aaagtcaaag caggtgcttt ttcacatgag atggatcata aaacaaaact acaagaagtc
    56821 ttgcaaaaat caggtgatgt atcaatcgaa taccgattga tcaatgaaga aggtccagct
    56881 catgaacgag tattttggat cgaagtttat gtagatgatc aattgattgg cactggacaa
    56941 gggaaatcga aaaaattagc agaacaagct gcggcggaaa atgcattggc agcactgtaa
    57001 aagtaagtga gggaaagctt tgtatttgaa acgaattgaa atagccggtt ttaaatcatt
    57061 tgcagacaag acagtcattg attttgaaaa tagcgtcaca gcagtggttg gaccaaatgg
    57121 cagcggaaaa agcaatatta cagaggctat ccgctgggta ttaggagaac aatctgctaa
    57181 aagtctgcga ggcggaaaaa tgccggatat catctttgcc ggttccgata cccgtaagca
    57241 gctgaatatt gcagaagtca cagtgatttt agacaatagt gatcattatc tgccattgga
    57301 gtacaatgag atcagtgtaa cccgccgtta tcgacggact ggggaaagcg agtttttcat
    57361 caataaacaa agctgcagat taaaagatat ccaagaattg ttcttagatt ctggattagg
    57421 aaaagaatcg ttctcaatca tttctcaagg aaaagtagaa gcgattttca gtagtaaacc
    57481 ggaagaccgt cgtgggatct ttgaagaagc agctggtgtc ttgaaataca agcagcggaa
    57541 aaagaaagca gaacagaagc tttttgaaac agaagacaac ctcagccgcg tccaagacat
    57601 catccacgaa ttggaagaac agttaactcc tttggccgcc cagagtgagg cagcaaagga
    57661 gtttttacgg ctaaaagaaa cactgactca aaccgatgtc tctttgatgg ttgctgaaat
    57721 caagacagca aaaaaagatt gggacaacaa acaagcacag cttgcgaaat tcaatctaga
    57781 attaggcaaa ctatctgagt cgatccaaga acaagaatcc attttggcga agcagcgtaa
    57841 agaaaatgcg caagccgatc gtctgatcga aaaaaatcag caagtattat tagatctttc
    57901 ggaaaaactg aagcagacag aaggacaaaa agatgttttg caggagcgga caaagcatac
    57961 acaaaaaagc tcgcaagaat atcaaacttc attagcagaa gcacaaaaaa aggttaaaca
    58021 ttttgaaaag cttcaagaaa gcttgatgaa agcagcagca gaaaaagaaa cagagatcca
    58081 aaaagctgaa gcaaatctca taaaaacaca gcaagaatta gaaaaatacc aaaaatcaac
    58141 gaaagaatta ttggcagaat taagagatca atatgttgat ttgatgcaag aacaagcagc
    58201 tgtaggaaac gaactgaagt atctagaacg tcaatatata caagaaacag caaaaagcaa
    58261 gcagacgtta gccaagcaat cggaagtaga agcatcagta gaccgattga tcttacaaaa
    58321 agaagaattg acacaaaagc aggcgcagct aaaatcatct ttaactgaaa caaaagagaa
    58381 gttagaaatg atccaacaaa acgggaaaaa gttccaagaa gcattagcaa aagaacagcc
    58441 taaaatgtat caattaatga atcaagtcca gcagctacgt gctcgtaaga aaagccttca
    58501 agaaatccaa gaaaactatt ttggttttta ccaaggggtt cgattggtct tgcagcataa
    58561 acagcagctt tcgggtattg tcggagcagt ggctgaactg atcgatgttc ctgcagattt
    58621 cacgttagct atcgaaacag ctttaggtgg ggcagcccag catgtcatcg ttgaaaatga
    58681 aaaagatgct cggcaagcca tcacgtattt gaaacagcaa agaggaggaa gagcaacatt
    58741 ccttccactg acgacaatca aaccaagaca attaccggct catatattga cacaagcagc
    58801 agctgtagaa gggtttatag ggattgccag tgaacaagtc agttatcccg atcaaatcca
    58861 aactgtggtt caaaacctgc taggaacgat ccttttagcc aaagacttaa ctagtgccaa
    58921 tgcaatcgcg caaacgatcc gctatcaata tcgagtggtt tccttagaag gtgatgtgat
    58981 gaatgccgga ggatccatga caggtggagc gaataaacga ggaaaccaag gcagtttgtt
    59041 tgtacaaaat caagaattga aacagttgac tagtgaattt gaagaagcag ataaacaact
    59101 ccaagcacaa gaaaaaaaag ttcaggagct gcaacaagaa acagctcgtt tagccgaaga
    59161 acaagaagtt ttgcgtacac gaggcgaaca attacgtttt gaagaacaag aagcgacaaa
    59221 tcagctacaa aacatcataa atgaacttga acgttttgaa aaagaaaagc aaatctcaac
    59281 ttttgagaca cgtgaactgc agcagtttat cgaagattac gaaaagcaaa cgaacgaatt
    59341 gaaagacaaa caaacagatc ttgaatcgca acgacaaaaa atcgacgaag aaatcaaatc
    59401 gttaagtcaa gaaagcgacc agatggaagc cagaagagca caagtacaaa gtcaaaaagc
    59461 acaagaacaa gcagatcttg ctgtcttgaa agagcagttt aatcatctgc agatccagct
    59521 ccgcggtgcc cgagtccaaa aagcagaggc aaccgaacgt caagaagcga tagaaaagca
    59581 gttggcgaca ttgacagcag acttttctga tcatgaagtc acagaagaaa gtttggaaaa
    59641 acaaattaac gaattatctg ctcagcgaga aacattaaaa gccgagttgg caaaagccaa
    59701 agagcagcga gatagaaggc aaaaggagat cgataagctt gaagcagtct tggcagagcg
    59761 taatcgtgag caaaaagcac gactgtcgga acaatccaaa ttagaagtcc aaaaagaccg
    59821 agctgaaatg attttagata atcatttgac ctatctgcag tcagaatatc agctgacatt
    59881 tgagaaagct tcccaagact atcaagaaac aaccgatatc gaagacagta gaacaaaggt
    59941 ttcatcctta aaagaacaga ttgaaaaact gggaccagta aacttaaatt caatcgaaca
    60001 atacgaacaa gtcagtgaaa gacatacatt cttagcaaca cagcgagatg atctgttagc
    60061 agcgaaaaac caattgtttg aaacgatgga tgagatggat gatgaagtgc ggacaagatt
    60121 caaagaagta tttgaagcta tccgtcaaga attcaaaatt gtctttccaa atatgtttgg
    60181 cggcggtaaa gctgaattag tactgacgga accaactgat ttgctgaaaa caggtattga
    60241 gattgaagta caacctcctg gcaaaaaact gcaaagtcta agtttgcttt ctggaggaga
    60301 acgagcactt acagccatcg cgttgctctt ttccattatc cgtgtctgtc ctgttccgtt
    60361 ttgtatctta gatgaggtag aagcagcgtt agatgaagcc aacgtgaaac gttttggtcg
    60421 ttacttgagc gatttccaag atgacacaca atttatcgta gtcacacacc gcaaaggaac
    60481 gatggtcgca gcagatgtac tgtacggagt gacgatgcag gaatccggtg tatcaaaaat
    60541 cgtttctgta cggatggaag atatcaatga agaggcgaaa atcaacgaaa cgaaagtgta
    60601 ggaacgaaaa tgatcaaatt agtagcaatc gatttggacg gtacattatt aaatgaacaa
    60661 aagctgattt cagatgagaa taaacaagca ttagcacaag caaaacagca aggggtcaag
    60721 atcgttctat gtacaggacg ccccttagct gcgatggcgc attatctgca agaattagga
    60781 ctggtagatg aaggtgacta cagcatcact ttcaacggcg gtttggtaca aaaaaatgat
    60841 actggtgaaa tcatggaaaa gaaagtgatg gaagtatcag atatccaacg cttatacaaa
    60901 ctggctcagc aattggatct gccactagat gttttgtctg acaatgtggt tttacagctg
    60961 cctagtgccc caaggaagca atccttgtac aatgtgttga ataagctgct gcaattccaa
    61021 ccagcggata taagcgatat tacagatgaa tttgtgctta ataaggcagt aatcgcttat
    61081 gaccaagaag aattagatcc taaaatcaaa gaaataccag cagaattcca tgaaatgtat
    61141 gagatcatca aaacaagaag tatgcttttg gaatttatgc caaaaggtgt tacaaaagct
    61201 tatggtattt ctttattggc aaaagatctt ggtttgaaac aagaagaaat catggcgatc
    61261 ggtgatgagg aaaatgattt accgatgatc caatatgctg gactaggcgt agcgatggag
    61321 aatgctgtgc ctttcgtcaa agaagcagca gatttcgtga cagccagcaa tgaagaaaat
    61381 ggcgtagcca aagtgatcca aaaatttgta ttagagtagg gggagtcaaa tgggattttt
    61441 tgataaaatc aagcgtgctt ttactggcga agagaaaaca gaagaagttg aagtacagga
    61501 gaagtacgac aagggtttag aaaagacgcg taaaacattt ggtgaacgga tgaatgagtt
    61561 gtttgccaat tttcgaacag tcgatgaaga cttttttgaa gaattagaag aaacacttat
    61621 cggtgcagat gtaggttttg aaacagcgat acgaattacc gaagcattac gtcaagaagt
    61681 gaaactcaga aatgcgaaaa aatcggcaga agtccaaaat gcgatcatcg agaaacttgt
    61741 ggatctctat gaagcggaag gaatcaatga agtaaatgaa ctaaatatcc aagaagacgg
    61801 actaacagtt attttgtttg tcggtgttaa tggtgtaggg aaaacaacaa gtattggtaa
    61861 acttgcccat cagttcaaaa acgaaggtaa aaaagtattg atggcagcag cagatacctt
    61921 ccgcgctgga gcaatcgacc agttagttgt ctggggcgaa cgcgctggtg ttgaagttgt
    61981 tcgtggcaat gcaggaggcg atccagcagc tgtcgtattc gatgctttgg aaagagcaaa
    62041 gacagagcag gcagatgttt tactagttga tactgcaggc cgactgcaaa ataaagtgaa
    62101 cttgatgaac gaattagaaa aaataaaacg agtaatccag cgggaattac cagaagcacc
    62161 ccaagaaaca cttcttgtat tagatgcaac cactggtcaa aacgcgatgg ttcaagcaaa
    62221 acaattcaaa gaaacaacag atgtgaccgg tcttgttttg acaaaattag atggtacagc
    62281 aaagggcggt atcgtacttg ctatccgtaa tgaattgcat ttaccagtga aattggtcgg
    62341 tcttggtgaa ggaatcgatg acttggagcc gtttgatcca aatgattttg tcgttggatt
    62401 gttcaaagga ttactaaaag aagaataacc catgtctaaa aagcaggagc tgtgacaaaa
    62461 gttgcagctc cttgtttgaa tgattgtttt taacatagct gtgtttttga tgccttcatt
    62521 tttatactta tttgtaagcg gttctcaaac aatttcgttc tttttcttag actttttgat
    62581 tataattaga ttcatatgaa ttggaggtca cttaatgatg aattttaagc aattattatt
    62641 gcatgtcaat gcgcgccctt ttatcgacca agctcgattt ggtatagaac gggaaggaca
    62701 gcgggttgat cttgcaggaa atctagcaaa aaccgatcat ccagcaatct ttggcgatcg
    62761 atcctatcac ccctatatcc aaacagattt tagcgaaaca caaacagaga tgatcacccc
    62821 tgttaccgat tctattcccg aattatttca gtatctcgct gctgtttatg atgtgactgc
    62881 tcgttctata ccgaaagaag agatgatctg gccattaagt atgccacctg ccttaccgga
    62941 aaaagacgaa gagatcatta ttgcaaaatt aaaaaatttc gaagatgtct tgtatcgacg
    63001 ttatttagca aaagaatacg ggaagcgaaa acagatggta agcggtattc atttcaattt
    63061 tgaatttggc gatgagttgc taagaacatt gttcagccat caggaagaat ttcaagattt
    63121 ttccgaattc aaaacagaac tttatttgaa aacagcgaga aactttatgc gctatcgctg
    63181 gatgatcact tatttattcg gtgcttcacc aatgagtgag aagaactact ttttagatga
    63241 atcacatccg caagaacctg ttcgcagtat tcgcaacagt gcgctgggct atacgaatca
    63301 tccaaatgtg aaagtttcct atgcttctat gaaacagtat ttagcagata tcgagcgaat
    63361 gatcgaagaa gggaaacttt cggaggagaa agaattttat acaccgcttc gtttccgagg
    63421 cggaaagaaa gtcgcagatt tagcaacaac aggtgttcgt tatattgaat tgcggaatat
    63481 cgatttgaat ccttatgcaa gattgggtat caatccagag caagttcggt tcttacaatt
    63541 attcctgatg tacatgttat ggacagaaga aaaagaagac tgtgaccaat gggtagcaga
    63601 aggtacgact cgaaataaca aagtagcact agaacaacca tcagatcaaa cagaatttca
    63661 tcaagaaggt agagaaattc ttgaaggaat gaagcaaatg ctggttgaat tggattggtt
    63721 ggattctctt tatttagtag aggaagcatt gactcaaatg gatcatcctg aacaaacgtt
    63781 agcagcgaaa ctctatcaag aagcacagct atcgagtcag caagaagttg ctgtggcatt
    63841 gggacatcaa tattataaag aaagccatga acgtccctat cagttggccg gttttcgcga
    63901 aatggaactt tctactcaga tatttatgtt cgatgccatc caaaaaggtg tccaagtcaa
    63961 agtattagat gagtcggatc aatttttgcg tctgcaattc caagaccatg tagaatatgt
    64021 gaaaaatgca aacatgacaa gtaaggacag ctatatcgtc ccacttatca tggaaaacaa
    64081 aacagtaacg aaaaaagtct taaaagaagc tgggttccgg gtaccaggag gcgcagaatt
    64141 ttcatccatg gaagaagcgg taaaagctta tccgagattt gccgagcagg catttgtgat
    64201 caaacctaaa tcaaccaatt acgggttagg tatcacgatt ttcaaggaag gagctagtct
    64261 ggaagattat caagcaggtt tagccattgc ttttcgtgaa gacagttcgg ttttagtaga
    64321 agaatttatg ccgggaacgg aatatcgttt ctttgtgatc gacggagaag tccaagccat
    64381 catgttgcga gtccctgcta atgttattgg tgacagtatc cgaacagtga aagaacttgt
    64441 tgaagaaaaa aatagtgacc ctttgcgtgg aacgaatcat cgtgcacctt tagaattgat
    64501 ccaattaggt gaattagaac aattgatgtt gaaagaacaa gggttgacga tcgaatcagt
    64561 tccccaagcc aatcaaattg tttatctaag ggagaactca aatatcagta caggtggtga
    64621 ttcgatcgat atgactgatg aattttctga agcttacaaa aaaatcgccg tttctgcggt
    64681 agaagcacta ggagctaaga ttagtggtat cgatttgatt ataccagata aagaaatcga
    64741 tcctacaact gataaaaaag catatgggat catcgaagca aatttcaatc cagccatgca
    64801 tatgcatgtt tatccttttg caggaaaagg cagaagattg acgatgaatg tattgaaatt
    64861 gttatatcct gaagtatttt aaaagaaaaa gcttttgaca gattgtccta tttgatgtca
    64921 atctgatcaa aagctttttt tgcatgtaaa aatttatgtg ttttttcgtt ttaaaataaa
    64981 cgatcccaga agcttttttt cttttttggt ttttcttttt cagctggcga agagttttct
    65041 tctgggtatt ttttttcgat atcaatcaca ggttcattta ctgcttcttt tgctgttaag
    65101 agcaatccga attcctcagg atttgcagtg acgtaatcgt tgactacagt gaaacggcta
    65161 ttgtttttcg ttgctagctg gatatattgg ttttggacag aaaggggtag agccccattc
    65221 aagagtatgg aagcatctgg atgttttttg agttctttta aaaagttttc tttgtttttt
    65281 ggttggatca tttcagcaac agtcatacta agatagcagc gttccctgaa tgtccctaaa
    65341 tatttatgct gttcttcagg gttcactaaa ggtgtaccat aaagtccctt atccaaatgg
    65401 ctttttacat catcttgtgc cataaaaaat accctccttc ttctctttgt ctttcttacg
    65461 attatagcat attcccaata atacctaaaa catatgaaaa agcggttgct tttgttcaac
    65521 gattcactta aaataagaat gaaaatcatt ctcaattaga aaggtggatt ggatgcgcta
    65581 cggacgacta gtagcattat tagttatttt aagtagtatt tcattgttca tcggagtatc
    65641 gaatgtacaa ttgtcagaac tgctccagtt agatgggcaa agcttacaaa tcttgctagt
    65701 gactcgtttg ccacgaacga tttgtttgat ccttgtggga gcgaccagta gtatttgtgg
    65761 gttgatcatg caacatttga cccaaaataa atttgtttca ccaacgactg cgggcacgat
    65821 ggatagtgcg cgtttaggta ttttagtggc tatgattttc ctacctggtg catccttgat
    65881 tgtccgatcc ttgacagcat tttgctttgc ttttgcagga acattgcttt ttttatccct
    65941 tacgcgattg ttcctccaga aaaatcaatt gatgcttccg ttgattggag tcatgttagg
    66001 gaatatcatt ggttcagttg ctactttttt tgcttatcaa ttccagttgg tacaaaatat
    66061 gacttcatgg cttcaaggga attttgcgac tgtgatacga ggaaattatg agttattgta
    66121 tgctgttgtt ccattgttgc tcttggtttg gctatttgct taccgattta cgatcattgg
    66181 tctgggcgaa tcgtttgcag taaatctggg aatagattac cagaagatgc agtttttagg
    66241 cattggctta gtatcattgg ctagtgcagt catgttgatc atggtaggaa gcattccttt
    66301 tatcggagtg atcattccga atcttgtctc tagaatatat ggagatcaag tgcacaaaac
    66361 aattggcgta actgctttag caggtagttt gttcttagta ttttgcgatc ttgtcgctcg
    66421 cgtgttgatt tttccttatg aaataccagt cagtgtgatc gttggaatca tcggtggggc
    66481 tatcttcctt tatcttctga taaggggacg acgatatgct taaaaaaaag ccatctattt
    66541 ttattggtat gcttgttctt gttttatttg ttacaggaat gtacttgacg atccatacat
    66601 atggaaactg gcattttgca tgtgcattga ggagtaagaa aatttttgca tttatccttg
    66661 tctcgctagc tgtcagcagt gccacaatca gctttcagac aattacgcat aaccagtttt
    66721 tgactccttc agtattggga cttgagtcgt tatatgtcct tgtccaaacg gtccttttct
    66781 tctttatcgg aggagtgacg atgcttcagc agacaggtac agtttttttc ttattgaacg
    66841 ttgggctgat ggtcgggctg agtctattgt tatttttctt tcttttgaaa aaaatgagag
    66901 cgaatctttt tttattactg atgtcaggga tgattttagg tacgcttttt ggtagtgtca
    66961 gtaccttttt acaagtcatc atggatccga atgaatatga tttgcttcaa ggcagattat
    67021 ttgcaagctt tggcaatatc cagagtcagt atcttttgcc tgcttctttg atcattatcg
    67081 gctgctggta cctgttattt cgatatcgac atgtgctaga cgttttccat ctcggaaatg
    67141 aacaagcagt aaacctcggg atagacacag atcgttttga atttttgatt ttatgttgtg
    67201 tcagtctttt gacagggaca tcaacggctc ttgtgggtcc agttactttt ttagggttta
    67261 ttgtcgcaaa tatcagttat cgtgtctttt ctacttatcg tcatggtcag ctatttatcg
    67321 gaagctttct ggtagctatg ctgtttctga caggaggaca gctcgcagta gagcagattt
    67381 tcggctggaa tacaacagtg agtgtcgtcg ttgaatttgt cggaggaatt tattttgtca
    67441 caaaattgat tcgcgaaagg aagagtatcc tatgattgat ttacaaaagg tcaataaact
    67501 gtaccatgga aaatcagtgg ttaaagatgt acagttaacg attcctgaaa agaaattgac
    67561 tgcttttatc ggtccaaatg gtgctggaaa gtcaacggtg ttgtcgatga tcagtcgttt
    67621 gataccaaaa gatacaggtg aaatctattt agaccataac gaagtaaagg cttggaaatc
    67681 caatgaatta gccaaaaaat tatcgatcct acgacaatcg aatgaaataa atgtccaaat
    67741 caccgtgaaa gaattagtag aatttggacg atttccttat acgaaaggaa aattgacgac
    67801 aaaagacgag gaaatcgttc aggaatcact ggaacacctt gggttagaag aaatagcgga
    67861 tcaatcgatt gacactcttt caggcggtca actccaacgt gcgtatattg caatggtgct
    67921 agcgcaagac acggattata ttttactgga tgaacctttg aacaatctag atatgaattt
    67981 tgcggttcaa atcatgcaga ttttaaaaaa tttagtacaa gaaatcggga aaacaattgt
    68041 tattgttctt catgatatca actttgctgc aagttatgca gacgaaattg ttgcaatgaa
    68101 agatggacag ttatttacgc aaggaacgac ccaagaaatg ataaaaaaag atgtattgga
    68161 tgaattatac gaaatggaca tacggatttg tgagattgaa ggccgcagat tctgtcttta
    68221 tttctaaaac taacattagg agagaagaga tgaaaaaatt agtagtaggt atcttgattc
    68281 tagcatcatt tggtttagca gcatgtggaa atagcaacaa caccagtcag gcagacacaa
    68341 aaaaaagcag cacacaaact gaaactacat tgacgataac agatagtaat ggagaccaaa
    68401 tcgaggtgcc aaataatcca gaaaaagtcg tcgtattcga caatggttct ttagatacga
    68461 tggatgctct tggtgttgga gataaggtag ttggagcagc tacaagcagc cttcctgagt
    68521 atctttcatc ctataaaaaa gtagaatcag caggtggaat aaaagaacca gacttagaaa
    68581 aaattaatca gcttcagcca gatctaatca tcatttctgg ccgacaaaga gattttcaaa
    68641 aagatctaag tcaaattgct ccgaccattt tcctttcttt agatgcgaaa aatccatggg
    68701 aatctttcca gcaaaatgtc actgctttag gggagatctt tggtaagcaa gaagaagcta
    68761 aaactcaatt agaggaatta tctagtgcaa tcgatcagac gaaaaagaaa gcagaagcta
    68821 ctgacaaaaa agcactagtc acacttgtga atgaagggca attatcagcc tacggatctg
    68881 gttctagatt cggtttcatc catgatttat ttggctttga acaagcagat gatcaaatcg
    68941 aagcatccac tcacggtcaa agtgtttctt atgaatatgt gctggaaaaa aatccggata
    69001 tcttatttgt agtagacaga acaaaagcta ttggtggaga cgacagcaaa gatgatattt
    69061 ctgccaatga actggtagca caaacgaatg ctggtaaaaa tcagcagatc atctcccttg
    69121 aaccagatgt ctggtattta agcgggggcg gtttagaatc aatgaaactg atgatcgaag
    69181 atgtgaatca agcatttaaa tagcataaat aacagaggat taaactctca tactatgtaa
    69241 caataatgtt tcttacattc ttaaatagca agagcctatc ctcaaagaag ttatcaaaaa
    69301 ccatccgtaa aatcgtattg atgattttac ggatggtttt tatttatttg agaaatcttt
    69361 tttcaacgtg aattcattat ctacatcata cgggttgatt ctagaatggg gagtggcaaa
    69421 aaatagtgtg atccattttt tcagcatcaa gatcgcctcc tttgatttga atcaaaggta
    69481 caaagcgaaa cttagataaa acttaaattt agatattttt attttgtacc gatctcttct
    69541 ttaaacgatg ttccatctgc tgcttctcgg caataagtcc actagactag aaaatatgag
    69601 aagctatttt ctgtccagcg ttcttattac tcaaagcaaa tcgcagttgt cacaatctct
    69661 tctaaacgat gttccatctg ctgcttctcg gcaataagcc cactagacta gaaaatatga
    69721 gaagctattt tctgtccagc gttcttatta ctcaaagcaa atcgcagttg tcacaatctc
    69781 ttctaaacaa tgttcaatca gctgaccttc ggtattagct caaagcactg atttcacaac
    69841 ctctatttag gcatgtgtct cagcaagcgg ttgatagttg atgatctgca tttttcgata
    69901 aaaatcggcg ggaagcttac tccaccgttt taataattga tagaaataac tctcttctgc
    69961 tttgatttta tactcgctta atatcaatcc aaaactattc gttttttttg aagctaattt
    70021 tttataaata tccaatgctt tggttttatc ttcattgaca aagagtgttt ttacttcaat
    70081 ataagaaccc tcgatatttt
//
LOCUS       DACDKM010000005        67822 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20834_2016
            SAMN12640370-rid9891663.denovo.005, whole genome shotgun sequence.
ACCESSION   DACDKM010000005 DACDKM010000000
VERSION     DACDKM010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640370
            Sequence Read Archive: SRR10040891
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 67822)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 67822)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742835.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742835.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:04:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,012
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 29 of 75
            Pseudo Genes (incomplete)         :: 33 of 75
            Pseudo Genes (internal stop)      :: 19 of 75
            Pseudo Genes (multiple problems)  :: 5 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..67822
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640370-rid9891663.denovo.005"
                     /isolate="E20834_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            162..392
                     /locus_tag="IV334_000321"
     CDS             162..392
                     /locus_tag="IV334_000321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1146 domain-containing protein"
                     /protein_id="HAP8942421.1"
                     /translation="MQVYGIDAVLRIVSHLAFIYLAFWSLRSLRIETFFRPMHNTQIR
                     MAIVLFSIFLGYTASNFFLEVIALCRNLFVSL"
     gene            489..1790
                     /gene="murA"
                     /locus_tag="IV334_000322"
     CDS             489..1790
                     /gene="murA"
                     /locus_tag="IV334_000322"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HAP8942422.1"
                     /translation="MEEIIVRGGNQLNGTVRIEGAKNAVLPILAASLLAEEGITTLDN
                     VPILSDVFTMNQVIRHLNVDVDFDEQKNQVTIDASRQLEIEAPYEYVSQMRASIVVMG
                     PLLARNGHAKVAMPGGCAIGKRPIDLHLKGFQALGAKIIQKNGYIEAIADELIGNTIY
                     LDFPSVGATQNIMMAAVKAKGTTIIENVAREPEIVDLANILNKMGAQVYGAGTETMRI
                     EGVDHLHAVNHSIVQDRIEAGTFMVAAAMTQGNVLIADAISEHNRPLISKLIEMGAEI
                     IEEEGGVRVIGPKHILPTDVKTMPHPGFPTDMQAQMTAIQLVAEGTSVVTETVFENRF
                     QHLEEMRRMNAHVKIDGNVAIMDGNHELQGAEVYATDLRAAAALVLAGLKANGITRVR
                     NLNYLDRGYYNFHIKLQQLGADVERVDMDQTSAEKTAQTIA"
     gene            1803..1979
                     /locus_tag="IV334_000323"
     CDS             1803..1979
                     /locus_tag="IV334_000323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HAP8942423.1"
                     /translation="MSNQRYILLTLIKILVVILLLILLFVAGTMIGYGVIGGGNPFKV
                     FQPSLWIHIRDFFH"
     gene            2114..2410
                     /gene="yidD"
                     /locus_tag="IV334_000324"
     CDS             2114..2410
                     /gene="yidD"
                     /locus_tag="IV334_000324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HAP8942424.1"
                     /translation="MKKILIYLVRGYQRFISPAFPPSCRYYPTCSNYMIKAIQTHGAL
                     KGTVMGTARILRCHPFVKGGIDYVPLTFQLTRNKKDKKEPDYKKVVKNTSSTER"
     gene            2474..2941
                     /locus_tag="IV334_000325"
     CDS             2474..2941
                     /locus_tag="IV334_000325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP8942425.1"
                     /translation="MIEELNKKDLPWSLLLDADPDKEKVQVYVSRGSGWIWKEKEKTI
                     GVLIYVAREKEYEIVNVAVAPTYQGKGIGGKLLETAFQKLSQLASSQTQIIIRTGSTS
                     SAALHLYQKMGYVEIGRVKDYFIHHYSEPIFENGDQLRDQVILAIHLPKNSFN"
     gene            3026..3910
                     /locus_tag="IV334_000326"
     CDS             3026..3910
                     /locus_tag="IV334_000326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HAP8942426.1"
                     /translation="MGILVFDFGGSAVKYGCWDGKEIKGKGQFATPESWEEMKAQLFQ
                     VYKKILVSFEVEGIGISSPGVVNNEKQVIEGISAIPYIHGFNIFRDLETLFQLPVTIE
                     NDANCAGMAEFYEGAARDYQDVAFVVAGTGIGGALFTKGQINKGAHLYGGEFGLMFLN
                     GDKTFSKLGTAVQMAWRYCERKGLDKNSYTGKDVFELAETGDAIAKEEVESFYTYLTK
                     GLFSIQFSFDPEVIVLGGGVSAKEGLIDEIKSRMKKLTEQFDLHDFEPQILLCEYRND
                     ANLVGAAANYLAVRQGEN"
     gene            4027..4260
                     /locus_tag="IV334_000327"
     CDS             4027..4260
                     /locus_tag="IV334_000327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2969 domain-containing protein"
                     /protein_id="HAP8942427.1"
                     /translation="MSKKNKDVEVRIEEVKKDIKGKNYEVTQLFIGKKMIGEILAYGP
                     KEYEIFFGEEDFGKEKSLENAIETVIRHWNLHE"
     gene            4348..4428
                     /locus_tag="IV334_000328"
     tRNA            4348..4428
                     /locus_tag="IV334_000328"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4382..4384,aa:Tyr,seq:gta)
     gene            4435..4506
                     /locus_tag="IV334_000329"
     tRNA            4435..4506
                     /locus_tag="IV334_000329"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4467..4469,aa:Gln,seq:ttg)
     gene            5021..5728
                     /locus_tag="IV334_000330"
     CDS             5021..5728
                     /locus_tag="IV334_000330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAP8942428.1"
                     /translation="MSNQLPVYIQIHDQLKNEIEQGIWKVGDRLPSERELAVKFNVSR
                     MTLRQAIQNLADEGILERKIGSGTYVAREKVQEKMSGATSFTEIMESQNRVPSSRTIS
                     YFLTSPSSSEMEKLNLSKDDTILRMERIRFADDVPICFEVASVPQKLIDGYSKAEITE
                     SLYRTLEEKGGKKIGPANQTITAVLASEKIADYLEVKRGDAVLRLRQISYFEDGTPFE
                     YVRTQYVGNRFEFYLEK"
     gene            complement(5788..6432)
                     /locus_tag="IV334_000331"
     CDS             complement(5788..6432)
                     /locus_tag="IV334_000331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YigZ family protein"
                     /protein_id="HAP8942429.1"
                     /translation="MIERYRTIKENGQSEIEIKKSRFICSLRRIESEDQAKSFIQEIK
                     KEHWKANHNCSAFIIGEKNEIQRSSDDGEPSGTAGVPMLEVLKKNQLINVAAVVTRYF
                     GGTKLGTGGLIRAYTHAVSQALSDIGIVEGTLQQEISVMITYPQLGKLQNYLELQHFF
                     IKEIQYTDQVSVICAVDEKSADHFKESIIELLNGQVQFEEGPVSYHEQLVTDHI"
     gene            6559..6846
                     /locus_tag="IV334_000332"
     CDS             6559..6846
                     /locus_tag="IV334_000332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8942430.1"
                     /translation="MQERMKKYDAITHYLKNNGGSQVTLTFTQFDELLFPSNGLPKTA
                     RESTDWWANDYKHPEKGAYGWINAGYEVVVINLDKEYVVFNKLVKSSWLFD"
     gene            6954..7274
                     /locus_tag="IV334_000333"
     CDS             6954..7274
                     /locus_tag="IV334_000333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAP8942431.1"
                     /translation="MAKKTIMLVCSAGMSTSLLVTKMQKAAEAKGIDSDIFAVSASDA
                     DNNLANKDVDVLLLGPQVRFMKADFEKRLEPKGIPLDVINMADYGMMNGEKVLDQAIT
                     LIGE"
     gene            7355..8668
                     /locus_tag="IV334_000334"
     CDS             7355..8668
                     /locus_tag="IV334_000334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HAP8942432.1"
                     /translation="MNHFWGRRLLTRDIKAIDCEEVNLPNYEKIAAVERLGNWILCHR
                     CGMRTSVIDGQLADYGYFCTHCLYLGRCDTQQDLYLFDQPEAKPREVVFFWTGQLTEK
                     QTEIAKRILCHSEKRHHLIWAVTGAGKTEMLYPILVETLKAGGRVAICTPRVDVCNEL
                     FLRYRYVFPEEKITLLHGNTATAYTFTSFVICTIHQLYRFYRSFDLIVIDEIDAFPYN
                     GDAGLAHAVQTAIKPDGRFIYLSATPDKKLLKEIKQTFDIHKLALRFHRRLLPEPVLV
                     FMNNWRQKCKNKKKIKSLVRIIHQLIKENHILLFCPSITMMHRLKATLQEALPRYEIT
                     DVSSRDNQRAEKVQKMREAKYDILLTTMILERGVTFERISVIVLGADHPVFTKSSLVQ
                     IAGRADRKGPYTNSQVYFFYEEKTAAIRKACQEIKEMNEEGKKML"
     gene            8665..9384
                     /locus_tag="IV334_000335"
     CDS             8665..9384
                     /locus_tag="IV334_000335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HAP8942433.1"
                     /translation="MRCSCCSKDIVQNLTFSEIIGVSKYRLPQTCSVCRAQFHLLETR
                     GCSGCQKPIHFNLESTKKKVKSIPVYCSDCQEWKKKYPTYDFQHRAFFVYDENMKAWL
                     NQYKFMGDIRLAGTFAYKWTQIKKEYPTYIYCPIPLSEKRRTERGFNQVSEMLKAARI
                     PFQMLLKREKHLLAQAKNTRKERLEMPQPFELAVTFSKIQNKKILIIDDVYTTGQTLF
                     HAADCLQVAQPKIIRTISLAR"
     gene            9512..10063
                     /gene="raiA"
                     /locus_tag="IV334_000336"
     CDS             9512..10063
                     /gene="raiA"
                     /locus_tag="IV334_000336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HAP8942434.1"
                     /translation="MFRYNVRGENIEVTEAIRDYVEKKVGKLERYFSDAPDATAHVNL
                     KVYTEKTAKVEVTIPLPYLVLRAEETSPDLYASIDLVVDKLERQIRKFKTKINRKSRE
                     TGMNTADATALFNEEDENENDSELDIVRTKRLSLKPMDSEEAVLQMNMLGHNFFIFED
                     AETNGTSIVYRRKDGKYGLIETD"
     gene            10285..12819
                     /gene="secA"
                     /locus_tag="IV334_000337"
     CDS             10285..12819
                     /gene="secA"
                     /locus_tag="IV334_000337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HAP8942435.1"
                     /translation="MANLIRSLIENDKKELKRLDSIAKKVEAYADQMAALTDEQLQAK
                     TAEFKGRYQKGETLDQLLPEAFAVVREAAKRVLGLYPHHVQLMGGIVLHDGNIPEMRT
                     GEGKTLTATMPVYLNALTGEGVHVVTVNEYLATRDSTEMGELYNFLGLTVGLNINSKS
                     SDEKREAYNCDITYSTNNELGFDYLRDNMVVYRHQMVQRPLNYAIVDEVDSILIDEAR
                     TPLIISGQAEKSTALYTRADNFVKRLKEEEDYKIDVQSKTISLTEAGIEKAEETFGLD
                     NLYDIENTALTHHLDQALRANYIMLLDIDYVVQDGKVLIVDQFTGRIMDGRRYSDGLH
                     QAIEAKEGVEIEDETKTMATITFQNYFRMYKKLAGMTGTAKTEEEEFREIYNIQVYQI
                     PTNRPVIRDDRPDLLYPTLQSKFHAVVQDIKDRYRKGQPVLVGTVAVETSELLSDMLN
                     QERIPHEVLNAKNHFKEAEIIMNAGQKGAVTIATNMAGRGTDIKLGLGVRELGGLAVI
                     GTERHESRRIDNQLRGRAGRQGDPGMSQFYLSLEDDLMKRFGSERIKSFLDRMKIGEE
                     DAVIQSKMLTKQVESAQKRVEGNNYDTRKNVLQYDDVMREQREVIYKQRQEVIMEEKS
                     LSKPLMNMVKRTISRVVDAHTQLEKENWNLESIIDFAGSTLVHEDSITLADIEGKTAQ
                     EIKEYLNERAQEVFNTKAAQLNGPEQLLEFEKVVILRVVDSKWTDHIDAMDQLRQSIG
                     LRAYGQNNPLVEYQTEGYKMFEDMVGAIEYEVTRIFMKAEIRQNVQREQVSQGTASHS
                     EDGDAKENSNTSAKKQPIHVNKVGRNDPCPCGSGKKYKNCHGKNAN"
     gene            12945..14046
                     /gene="prfB"
                     /locus_tag="IV334_000338"
     CDS             join(12945..13007,13009..14046)
                     /gene="prfB"
                     /locus_tag="IV334_000338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_078989099.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HAP8942436.1"
                     /translation="MEISEIRNLLTEMQEKVTSFRGLFDLDQLEEDIAEAEAKMAEPG
                     FWDDSEAAQKVISENNANKETYDQFHSLAEELEEIELMVEMLQEEPDAAMQEEAETRI
                     NALDEKMKTYELSMLLDGPYDRNNAIVELHPGAGGTESQDWGSMLLRMYTRWAEQHGY
                     QVETLDYQAGDEAGIKSVTLLIKGHNAFGYLKSEKGVHRLVRISPFDSAKRRHTSFCS
                     VDVMPELDETIDIEINPDDLKIDTYRASGAGGQHINKTESAVRITHLPTGTVVASQAQ
                     RSQLKNREQAMGMLKAKLYQLEVEKKEQEAAALRGEQKEIGWGSQIRSYVFHPYSMVK
                     DHRTNVETGNVQAVMDGDLDLFIDGYLKMHLQ"
     gene            14151..14837
                     /gene="ftsE"
                     /locus_tag="IV334_000339"
     CDS             14151..14837
                     /gene="ftsE"
                     /locus_tag="IV334_000339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005237293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HAP8942437.1"
                     /translation="MIEMKDVMKKYSNGTTAIRNLSIVIEQGEFVYVVGPSGAGKSTF
                     IKLMYREEKASKGTLNVAGHDLIAIKNREVPYLRREIGVVFQDYKLLPRKTVYENVAY
                     AMQVIGRKPRDIKKRVMEVLDLVGLKHKVRVFPSELSGGEQQRVSIARAIVNTPKVLI
                     ADEPTGNLDPENSWEIMKLLDRINAQGTTVVMATHNSTIVNTIRHRVIAIENGRIIRD
                     QAEGEYGYDD"
     gene            14821..15714
                     /locus_tag="IV334_000340"
     CDS             14821..15714
                     /locus_tag="IV334_000340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP8942438.1"
                     /translation="MDTMIRTFFRHLLESIKSLKRNGWMTVASVSAVTITLTLVGVFM
                     AVIMNATKLAQDIEGNVDVSVFVDIGTKDEEMKKLEKELNDLDHVKKVEFSSKEQELK
                     KIQNEMGDSWKLFEGDNNPLYDVYVVSATDPSYTKQVAEEAADLKNVSRSDYGGASSD
                     RIFQIAGAVRTWGLVAAALLLFVAMFLISNTIRITILSRQREIQIMRLVGAKNGYIRW
                     PFFLEGGWIGLIGAILPILIITFGYAWFFQLINPSLLRSHYSLIHPQNIVWKINLLMV
                     GIGVIIGSLGSIISMRRFLKF"
     gene            15866..17314
                     /gene="cls"
                     /locus_tag="IV334_000341"
     CDS             15866..17314
                     /gene="cls"
                     /locus_tag="IV334_000341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HAP8942439.1"
                     /translation="MKIIVDNFFTILLIMNILLSFIIVFRERKETAQTWAWVLVLMFI
                     PVVGFILYIFLGRGISKEKIFDLKIQGKIGKNLEIEEDKQALMRGLYPHPPTGQVDVK
                     QLIYMLTVFESTLYTTKNEITLFTDGREKFDALIEDIKQAEDHIHFQYYIYRSDALGE
                     EVRDALTEAARRGVKVRVLLDAWGSTQVSSSFFQNLKKAGGEVAFFFPLFVPYINPRI
                     NYRNHRKIVVIDGKIGYTGGFNVGNEYLGLVKKFGYWRDNHLRIYGEAVYTLQNRFLM
                     DWNSQHTKEVGYSPKFFPSIHSTGDIAVQIVTSGPDTEHEQIKMTYLKMISLAKREIL
                     IQTPYYIPDGSIHEALKLALLSGVKVHIQIPNKPDHLLVYWATYSFAAELIEYGARIE
                     TYENGFIHAKTMIIDGGIVSVGSANIDVRSFRLDFEVNTLVYDERMASRVRKAFFEDS
                     KISTHLTKEMYENRGIIIKMKEGLARLISPLL"
     gene            17651..18508
                     /locus_tag="IV334_000342"
     CDS             17651..18508
                     /locus_tag="IV334_000342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HAP8942440.1"
                     /translation="MKKLKLFLPIIGLAILLSGCSKWIDRGESITAVGSSALQPLVET
                     VAETYQTENPGKFINVQGGGSGTGLSQVQSGAVDIGNSDLFAEEKSGIDASALVDHRV
                     AVVGITPIVNKDIGVKDISMENLKKLFTGEIKNWSEVGGKDQPVVILNRASGSGTRST
                     FEKWVLDGETAVQAQEQDSSGMVRQIVADTPGAISYVAFSYVSDDVDTLNVNGVEPTD
                     ENVTTNKWTIWAYEHMYTRDHPKKLTKEFLDYILSDEVQDNIVGELGYIPVSHMKVER
                     DWQGNIVKE"
     gene            18623..19543
                     /gene="pstC"
                     /locus_tag="IV334_000343"
     CDS             18623..19543
                     /gene="pstC"
                     /locus_tag="IV334_000343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HAP8942441.1"
                     /translation="MEDVKKVLLTKSKRSKMEQRGKFISFLCIALIVLVVLSIFYFVA
                     SKGLATFFTDKINVFDFLFGTDWNPSQIGANGKPMVGALPMITGSFIVTFLSAIVATP
                     FAIGAAVFMTEISPKNGTKILQPVIELLVGIPSVVYGFIGLSVIVPFVRSIFGGTGFG
                     ILAGTFVLFVMILPTVTTMTVDALKAVPRHYREASLALGATRWQTIYKVVLRAAVPGI
                     LTAVVFGMARAFGEALAIQMVIGNAALMPTGLTTPASTLTSILTMGIGNTIMGTVENN
                     VLWSLALILLLMSLFFNIVIRMIGKKGELK"
     gene            19543..20427
                     /gene="pstA"
                     /locus_tag="IV334_000344"
     CDS             19543..20427
                     /gene="pstA"
                     /locus_tag="IV334_000344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010764435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HAP8942442.1"
                     /translation="MNAKRSDKIATGVLYIVSGIIVLILAALLLYILVRGIPHISWSF
                     LTEPSKAYQEGGGIGIQLFNSIYLLLITMIISFPISLGAGIYLSEYAGKNWLTDVIRT
                     SIEILSSLPSVVVGLFGFLVFVIQFQYGFSIISGALALTVFNLPLLTRTVEESLQAVH
                     YTQREAGLALGLSRWETVIKVVVPEATPGILTGVILSSGRIFGEAAALIYTAGQSAPA
                     LDFTNWNPLSVSSPISIFRQAETLAVHIWKINTEGTMPDGVSVSAGASAVLIIAVLLF
                     NFGARAIGKRLYRKMTSA"
     gene            20442..21248
                     /gene="pstB"
                     /locus_tag="IV334_000345"
     CDS             20442..21248
                     /gene="pstB"
                     /locus_tag="IV334_000345"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HAP8942443.1"
                     /translation="MKKYNWNETNVITMPKEKEVALYTDDLHVWYGDNEAIKGVDLEF
                     EKNKITALIGPSGCGKSTYLRSLNRMNDGIANTKITGKIMYQGVDINAPQVDVYEMRK
                     RIGMVFQRPNPFSKSIYDNITFALKQHGEKNKKKLDEIVETSLKQAALWDQVKDSLDK
                     SALALSGGQQQRLCIARAIAMKPDILLLDEPASALDPISTGTVEETLVNLKEDYTIII
                     VTHNMQQAARISDYTAFFYLGKVMEYDKTNKIFTRPKIQATEDYVSGHFG"
     gene            21264..22022
                     /locus_tag="IV334_000346"
     CDS             21264..22022
                     /locus_tag="IV334_000346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HAP8942444.1"
                     /translation="MTKEIISSKDLHLYYGKKEALKGIDLSFNQGEITAMIGPSGCGK
                     STYLRSLNRMNDLIPNVTITGSVLYKDKDIYGPKTDVVELRKEIGMVFQQPNPFPFSI
                     YENVIYGLKLKGEKDKKVLDQVVEESLKAASVWDDVKDKLHKSALSLSGGQQQRVCIA
                     RVLAVNPEIILLDEPTSALDPVSTGKIESMLLELRERYTMIIVTHNMSQASRISDKTA
                     FFLDGHLIEYNDTKKIFMNPEKQETEDYISGRFG"
     gene            22035..22712
                     /gene="phoU"
                     /locus_tag="IV334_000347"
     CDS             22035..22712
                     /gene="phoU"
                     /locus_tag="IV334_000347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010723432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HAP8942445.1"
                     /translation="MLRTQFEEELLNLHNQFYEMGMMVSSAVHKSVRAYIKHDKILAQ
                     EVIENDININNMETRLEKKSFEMIALQQPVTTDLRMIITVMKASSDLERMADHAVSVA
                     KSTIRVKGQTRIPEIEKEISDMSDYVKKMVDNVLVAYVKTDEKDARTIANMDQRVNDY
                     FNRIYNATIKNMQENPETVISGTDYLNVASYLERIGDYVTNICEWIVYLATGKITELN
                     TNHNEIF"
     gene            22950..23981
                     /locus_tag="IV334_000348"
     CDS             22950..23981
                     /locus_tag="IV334_000348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibE/F family protein"
                     /protein_id="HAP8942446.1"
                     /translation="MQKKISIITIFFICVLTSWVIQHQAALYEVPVIEIEKVRIKEDH
                     QLITGKLMNTDQKGESIYLSVPYQKNQIETANIKARQMYLVQMHGDEWQLVAKKNDGW
                     LFFFTSIFIGAMLLIGGKQGLFALGSLIINIVLLLLLLLIFTKTEGISLLNISILFVV
                     IGMIISILALDGWNRSSIIKISAAVTSVFLAFFICLLTMNHWKDQGLRYEELNYLTRP
                     YRSVFLSSVLIGTAGGTLDTVITVIATLEELTKRQPEITAKQLWLSGRQVGRDVIGSM
                     TNILLFVYISGSVPSLLLYLGNQWSFKETIEQHLSLEFLRVIAGSLGIVLSIPISVLF
                     FLTVRRLKR"
     gene            23978..24745
                     /locus_tag="IV334_000349"
     CDS             23978..24745
                     /locus_tag="IV334_000349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibE/F family protein"
                     /protein_id="HAP8942447.1"
                     /translation="MNVLIILVGILAISVLLIGGTQGMYILLGLFINLGIFFLLLFGY
                     HQKWPILALSVIGFLLIAVVILFFINGYNLKMRAAFVSILIFLFCFLLLLPITDFLAI
                     QGFTSVELEELSGLDKTLAIDFRLLTRSLLLISLSGAVLDASVAISSGTFEVYQANPQ
                     LSFYQLRHSSFAIAKKILASTVMTLLFAFMGSSLALILWFIDLDIPFQQVINEKSFVL
                     EYTMAILTTLSALLVLPLTCVTTAYLFTKKKEQLKME"
     gene            24845..26413
                     /gene="liaX"
                     /locus_tag="IV334_000350"
     CDS             24845..26413
                     /gene="liaX"
                     /locus_tag="IV334_000350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="daptomycin-sensing surface protein LiaX"
                     /protein_id="HAP8942448.1"
                     /translation="MKERERILDLVKKGILSTEEGLDLLESMATEKDEKQIKKEADKV
                     TASHKEKDQASQLIDKLENGEEEISEPVDPKERERQDQENLEKILDELATEANKTSAR
                     LDEVNAQFADNKAARNEKQEALMQLNTKEELGELTEEELAQRQTLEAEIKELEASGDT
                     LLEEQLKLEAELKDIRKNQWSEKKETFTDKFELPDDWKDQATDTLNQVGEKMSEAGSQ
                     LGKFLKKTFQTVSETVNDNMEWKDVSLRVPGIATTKFEHEFYYEAPAASILDIKAANG
                     NVTLKTWDSNDVKVEAKIKLYGKMGAEPFEAFSERSQIEVNEDHISFQIPNKRVRADL
                     VFYLPKRVYDHAAIKLLNGNIMIETLEAKDIYTKSTNGNIIVDQLTATMLEVEGVNGN
                     IDIRNGNILDSIIETVNGTVTFGATAENLSVSLVNGDVRLTIKEDNLKKVEASSVNGN
                     VKVALPDGIGLEGHAKTSLGSINSRLSNYEVVREKKERTNQMLQFRRVSDGEIAQVQL
                     STTTGSIYLKDTDK"
     gene            26713..27030
                     /locus_tag="IV334_000351"
     CDS             26713..27030
                     /locus_tag="IV334_000351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HAP8942449.1"
                     /translation="MKKKLTKSNKNVVLTGTLAGIGEYIGIDPTVIRVLYVFLSLVAF
                     GSPIILYILLALIIPSARSARTNYGHNNAYYEKNNYREQTREQTKKRKEAEKIDDDEW
                     SDF"
     gene            27030..27380
                     /locus_tag="IV334_000352"
     CDS             27030..27380
                     /locus_tag="IV334_000352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HAP8942450.1"
                     /translation="MSYFQRILVNTLTFISLAVLFPAMVHVSSIWTAVLASFVLSVLN
                     MFVKPILTILSLPFTLVTLGLFTFVINAAILKMTSFFVGAEHFSFSSFGAALLVAILM
                     SLVNVIVSEHNSRK"
     gene            27547..28485
                     /locus_tag="IV334_000353"
     CDS             27547..28485
                     /locus_tag="IV334_000353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr kinase/phosphorylase"
                     /protein_id="HAP8942451.1"
                     /translation="MSEVVKVSQLVKKLSLEIVTGDEKSLDRVIVTGDISRPGLELTG
                     YFNYYSHNRLQLFGSKEISFAERMIPEERLIIMRRLCSEDMPAFIISRGLPAPEEMIQ
                     AANENGIAVLRSPISTSRLLGELSSYLDGRLAPRTSVHGVLVDVYGLGVLIQGDSGIG
                     KSETALELIKRGHRLIADDRVDVYQQDELTVIGEPPKILEHLIEIRGIGIIDVMNLFG
                     ASAVRGSMQVQLAVYLEAWAKDKKYDRLGSEDTTVEIAEVGIPQIKIPVKTGRNVAII
                     IEVAAMNFRARTMGFDATKTFEERLSRLIEENSGND"
     gene            28500..29330
                     /locus_tag="IV334_000354"
     CDS             28500..29330
                     /locus_tag="IV334_000354"
                     /EC_number="2.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HAP8942452.1"
                     /translation="MLAQINRVAFELVGIPIYWYALIIVSGIIIAMWLSSREAVRVGL
                     KADDVTDFMLWGLPISIIGARLYYVLFDLPQYIADPIQIFNIRSGGLAIYGGLIAGAI
                     TLYFFTKYHFISLWTFLDIAAPSVLLAQAIGRWGNFMNHEAYGPDTTRSFLESLHLPQ
                     FIIDNMYIEGIYRQPTFLYESLWSLIGFILLLFLRKKPHLLKKGEVLLCYLMWYSFGR
                     FFIEGLRTDSLYFLGVIRISQLLSAVLFVGTLILFIWRRKKGNLPDYDRSLGKNQQII
                     "
     gene            29374..30399
                     /locus_tag="IV334_000355"
     CDS             29374..30399
                     /locus_tag="IV334_000355"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HAP8942453.1"
                     /translation="MNYRVAVLGPGSWGTALAQVLAENGHDVRIWGHRASQVEEINTQ
                     HTNQRYLPDHYLPDSIQAFTNMEDAVKDADAVLFVIPTKAIRSVAKELVPKLNTKPVI
                     IHASKGLEQGTHKRISDILLEEIPAEKRKAVVVLSGPSHAEEVAKHDITTITAASTDA
                     KAAKYVQELFMNNYFRIYTNPDVIGVEMGAALKNIIAIGAGAIHGLGFGDNAKAAIMT
                     RGLAEISRLGVAMGANPLTFIGLSGIGDLVVTCTSVHSRNWRAGNLLGQGQPLEEVLA
                     NMGMVVEGVNTTQAAMELAQMMEIEMPITQTIYEVLYEQKEIKTAAKEIMLRDGKKEN
                     EFSLTIF"
     gene            30414..31352
                     /gene="galU"
                     /locus_tag="IV334_000356"
     CDS             30414..31352
                     /gene="galU"
                     /locus_tag="IV334_000356"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HAP8942454.1"
                     /translation="MKVKKAVIPAAGLGTRFLPATKAMAKEMLPIVDKPTIQFIVEEA
                     LKSGIEDILIVTGKAKRPIEDHFDSNIELETNLSEKGKTELLKLVEETTDVNLHFIRQ
                     SHPKGLGHAVLQARAFIGNEPFVVMLGDDIMEDEVPLTQQLIDDYERTHASTIAVMQV
                     PHDETSKYGIINPGEVLEDGLYNVKNFVEKPDPSKAPSDLAIIGRYLLTPEIFDVLSE
                     QQPGAGNEIQLTDAIDTLNKTQRVFARKFTGKRYDVGDKFGFMKTSIEYGLTHPEVGA
                     PLREYILSLGKTLEAEGSKIDHNPKGITEIKEEKKK"
     gene            31477..31917
                     /locus_tag="IV334_000357"
     CDS             31477..31917
                     /locus_tag="IV334_000357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF948 domain-containing protein"
                     /protein_id="HAP8942455.1"
                     /translation="MSGGEIAGLIAAIAFAILVVFIVRVLLQVSKTLEKVDQTVAEAN
                     TTIEIVTKDVDLLSRQVEGLLVKSNELLLDINGKVATIDPLFTAVADLSESVSELNHS
                     SKNIATKVGGIGKTTAKATVAGKVGGTAMKFFKNKKHTETTGGK"
     gene            31920..32522
                     /locus_tag="IV334_000358"
     CDS             31920..32522
                     /locus_tag="IV334_000358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HAP8942456.1"
                     /translation="MAKKGGFLLGAVVGGTAAAIAALLLAPKSGKELRDELAAQADDL
                     MDMASDYTDMAKEKSTELTGIAKEKAQDLSQQAGDLAGSVKQKVSESADEAGSESDDI
                     LLNLKEQSADLSNRFKKTAEDMKKPADELGSIAKDTSEDIMIDVKDAAGKAGDTVNEG
                     MSEAKPVADDSKEMMKMAKEDAKEAKKEIKDTMTDSSTNH"
     gene            complement(32581..33687)
                     /locus_tag="IV334_000359"
     CDS             complement(32581..33687)
                     /locus_tag="IV334_000359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HAP8942457.1"
                     /translation="MNNVKINELRQWMAQHQVELAYISDPGHIAYFSGYESDPHERVL
                     ALFIPLEQDPFLFTPALEVEDAQNSSWAYDVRGYLDSENPWEIIGKELRARYGQPGNV
                     GIEKNALTVERFDAISDILTGTDSYTDLTSIIQRLQLIKTLEEKDKLIEAGKWADVAF
                     EIGFKAVKEGVTEQAIIAEIEYQLKKQGVSQMSFDTLVLAGANAASPHGTPGNTKISP
                     NELVLFDLGVVWNGYCSDATRTIAYQKPTDFQEKIYNITLEAQLAAQEAVRPGVTAGE
                     LDQIARNVINSYGYGEYFNHRLGHGIGTTVHEFPSLVEGNDLVIEEGMCFSLEPGIYI
                     PEKVGVRIEDCVYVTSDGCVPFTTTPKELLVLEK"
     gene            33897..34898
                     /gene="ccpA"
                     /locus_tag="IV334_000360"
     CDS             33897..34898
                     /gene="ccpA"
                     /locus_tag="IV334_000360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catabolite control protein A"
                     /protein_id="HAP8942458.1"
                     /translation="MEKQTITIYDVAREANVSMATVSRVVNGNPNVKPATRKKVLEVI
                     DRLDYRPNAVARGLASKKTTTVGVIIPDVSNMFFASLARGIDDVATMYKYNIILANSD
                     GNDQKEVNVLNNLLAKQVDGVIFMGHHITDEIRGEFSRSKTPVVLAGSIDPDEQVGSV
                     NIDYVSATKDAVNKLAKSGNKKIAFVSGALIDPINGQNRLKGYKEALSENNLPYSEGL
                     IFEAQYNFKDGLSLVDRIHNSGATAVYVSDDELAIGILDGLLDRGVKVPEEFEIITSN
                     NSLLTEVARPRLTSITQPLYDLGAVSMRLLTKMMNKEEVEEKTIILPYGIEEKGSTK"
     gene            complement(35235..37580)
                     /locus_tag="IV334_000361"
     CDS             complement(35235..37580)
                     /locus_tag="IV334_000361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HAP8942459.1"
                     /translation="MPRKNTRKKQNRRKKEKWFVPKIIFRVFQSLTVFITVLLLMFAA
                     LGIGIGAGYFAYLVEDTQLPTKKALQTELGNITETSKIVYADNTEISKIQTDLMRTTI
                     SSDKISPLLKTAIISTEDEYFDKHQGYVPKAVLRALVSEATGIGSSGGSTLTQQLVKQ
                     QILTDETTFKRKANEIILSAQVEKYFSKDEIIATYLNVSPFGRNNKGQNIAGVQEAAK
                     GIFGVDAKDVTLPQAAYIAGLPQSPITYSPYTNTGALKEDLSAGLARKDFVLFSMYRE
                     GQITKEQYEEAKAYDLTKDFLPQQIAEQTDREFLYYTVMNEATRIIAQQLAEKDNADM
                     SDSSVSDAYYQKAQQTIQNKGYTIHSTIDKDVYAALQNGVENYGYMLDDGAGSQVETG
                     NVLMDNRTGRIYGFVGGRNYSQNQNNHAFDTERQAGSSIKPVLVYGPAIDMGLIGSES
                     RVSDYATTWQEGENAGEKIVNATNEGSNTFQTVRESLEWSNNIPAYHLYQDVLNSGGS
                     KQYAYEHYLAKMNYPANDNWGVESAPLGTVDVTTLQQTNGFQALANGGVYEEGYIIDS
                     ITDNAGNVIYKHESNSVRIYSEATASIMNDMMRSVINAKITTPFKDAISSLNGNLGKA
                     DWVGKTGSTNEYRDSWLIVSTPSITISSWAGHDDNTGMDSKARIRSANYLANLINQAY
                     QAKPDIFGTDEKFELSSDVMKKKVSGFTGQTPGKVTVNKKSIQTPGKTVESLWAKNGP
                     KKSAFKFGVGGTDENYTDYWNTASAYARANQATKESDEKDD"
     gene            37859..38761
                     /locus_tag="IV334_000362"
     CDS             37859..38761
                     /locus_tag="IV334_000362"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HAP8942460.1"
                     /translation="MLLGSHVSMSGKKMLLGAAEEAASYGSSTFMIYTGAPQNTRRKA
                     IEEMNIDAGKAYMAEKGLTNIVVHAPYIINLGNTIKPEMFPFAVQFLREEIVRAEALG
                     ATQITLHPGAHVGAGTEAGIARIIEGLNEVLTKEQTAQIALETMAGKGTEIGRTFEEL
                     AQIIDGVKLNEKLSVTFDTCHTNDAGYNIKEDFSGVMEEFDKIIGLDRLKVLHINDSK
                     NPQGSHKDRHANIGFGTIGFDTLNKIVHDPMFDNISKILETPYVGKDKKKAYAPYGKE
                     IAMFKAQTFDPEVFPELLAEEVIL"
     gene            38974..40749
                     /locus_tag="IV334_000363"
     CDS             38974..40749
                     /locus_tag="IV334_000363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosyltransferase"
                     /protein_id="HAP8942461.1"
                     /translation="MLNTLKPLINQLKKKHTYFILGIALLFTIIRLYLYSKAIYGTDM
                     SATYDDQLLIRYAQNILDGKWLGEYTTTTLSKGISYSLFLVLAHKLHLSYAVLFGMFS
                     IFASILVALAFKPIVKNKYLLLSIYFYFLFSPISFTHEYSTRTYRNALVIPAVEIIIA
                     CLLAIYYRKYGSSRKLLPWFLGLSVAFPFFWFIREDSIWLLPFTVVALGISFVQILLG
                     QDFSFKEVKKLKKRLFLLSKREWLKLSLFLLPIVGFSIVYQWIEYKNETTYGIATIND
                     RSSGEFGRLMKQLIRIDDETNLNKENSEIWVSHAALNKALAASPTFHTVSTRIDELYT
                     THAWTQGGKVKELPGDIIFWALRDVVSESGYYQNNAKETNDFWKKVNQELEEAYHKGT
                     IKKKKEFYLMGSGDGKTMSDLPIVFDFLQSGYAYAWRYKDFHQGGNYSFGTQEQVKQA
                     QELLNLPLMENWIDSNEPRTPNFQLTKSAKVANVIIKIYQVLAIPVLIASIAGILLMI
                     LGTVFAKADRAYYSSSLIVVTGLILTQLVFLFGVSWFCSYAPEKKDYFLSVYTGAGVP
                     LTQFIVVTSMLVFSKLNLKKALSSK"
     gene            complement(40815..42401)
                     /locus_tag="IV334_000364"
     CDS             complement(40815..42401)
                     /locus_tag="IV334_000364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divalent metal cation transporter"
                     /protein_id="HAP8942462.1"
                     /translation="MNNQSNKQFHKHRLIEHTNGLSLAEVNGTVDVPKGKSFFKTLLA
                     YSGPGALVAVGYMDPGNWSTSITGGQNFQYLLMSVILMSSLIAMLLQYMAAKLGIVSQ
                     MDLAQAIRARTSKTLGVILWILTELAIMATDIAEVIGAAIALYLLFDIPLVIAVFITV
                     LDVFVLLLLTKVGFRKIEALVVCLIFVILFVFVYQVGLSNPDWHSLFAGFIPNSHTFS
                     DSPVIGGETPLTGALGIIGATVMPHNLYLHSAVSQTRKIDHQNEEDVAQAVRFSTWDS
                     NIQLSMAFLVNALLLIMGVAVFKTGSVQDPSFFGLYEALSDPSAMSNGILIAVAKSGI
                     LSTLFAVALLASGQNSTITGTLTGQVIMEGFIHMRIPIWLRRLVTRLLSVIPVLICVL
                     FTSTKGTIEEHAALNNLMNESQVFLAFALPFSMIPLLMMTNSQAEMGSRFKNSFIVKL
                     LGWFSVISLTYLNLRGLPGQIESFFGEQATSAQLALADHIAYVIIVLVLLLLAWTIGD
                     LYKGNKKYEQLLQETVEEIE"
     gene            42652..42876
                     /locus_tag="IV334_000365"
     CDS             42652..42876
                     /locus_tag="IV334_000365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YneF family protein"
                     /protein_id="HAP8942463.1"
                     /translation="MLSTGIVVLIAIIALLIGAVGGFFLARKYMKDYLEKNPPVNEDM
                     LRSMMMSMGQKPSEKKIRQMMQQMKNQGKK"
     gene            43018..44769
                     /locus_tag="IV334_000366"
     CDS             43018..44769
                     /locus_tag="IV334_000366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAP8942464.1"
                     /translation="MSIFRKLGWFFRQEKKHYLIGVFSLFAVALVQLVPPKVIGIIID
                     EIADKNLSIKIIFGWIFLLIIAAFLQYLFRYIWRTNIWGSAARLEKELRQQLFKHFTE
                     MDNLFYQKYRTGDLMAHATNDLNAIQNVAGAGILTFADSVITGGTTIIAMILFIDWRL
                     TLIALIPLPLLAVTSRVLGSKLHDAFRDSQEAFSSINDKTQESITGIKVIKTFGQEKE
                     DLADFEEKIEDAIIKNKRVNFLDSLFDPFITLIIGLSYVATIILGGKYVLEGTISIGQ
                     MVSFISYIGMLVWPMFAIGRLFNVLERGNASYDRVNELLHEKTHIVEQLGAVQTPAKG
                     ELNVAISSFNYPKEESPALEQIKFAIQEGETLGIVGKTGAGKTTIIKLLMREYDDYQG
                     KIQFGGRSIKEYSLNALLGSIGYVPQDHFLFSMTIRDNIRFSNPKLSEEEVEHAASLA
                     FINNEIKELPKGYDTLVGERGVSLSGGQKQRISIARALIKNPELLILDDALSAVDAKT
                     EEAILHNLKEIRKEKTTIIAAHRLSSVMHAKEIIVVDQGKIIERGTHHELLALGGWYE
                     QMWEKQQLEAKIEGGEA"
     gene            44769..46553
                     /locus_tag="IV334_000367"
     CDS             44769..46553
                     /locus_tag="IV334_000367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP8942465.1"
                     /translation="MDENYQSEWTKSIPLKEQKKIIKRLMKFAKPFRRTFIVAILFAF
                     ALSVINVLLPRIIQTFMDDHLAKQSATTQVILFFAGMYLFGVIVKSIIWFFQWYLYSM
                     ASLKTYQYIRVKLFEKLHTLGMRYFDQTPAGSTVSRVTNDTETLFEFWYVFLMVLTGI
                     FAVVSSFLAMFQINGEIALWTLVFLPILGIVIWYYQKFSSRIYRNMREKLSQLNTKLN
                     ESISGMRIIQQFRQEARLAKEFEQVNNEYLDTRYAMIKTNSILLSPIINFLYALAIAL
                     VLTLFGIDALHSPVEVGLIYAFTTYVQGFFNPMSQMMDFLSIFTDGMVAGSRILKIMD
                     TEEYAPQQNPGADQMITSGKIEFRNVSFSYDGEHNVLNNISFVANPGETVALVGHTGS
                     GKSSIINVLMRFYEFYEGQILIDDHDIREYPMEELRKKMGLVLQDAFLFYGDIAGNIR
                     MMNPAITDEQIRHAAEFVQANKFIDELPKGYHSKVIEHGASYSSGQRQLITFARTIVT
                     DPKILVLDEATANIDTETEGLIQEGLAKMRQGRTTIAIAHRLSTIRDAELILVLDKGH
                     IVERGNHETLLAQNGLYADMYKLQSSGI"
     gene            46879..47730
                     /locus_tag="IV334_000368"
     CDS             46879..47730
                     /locus_tag="IV334_000368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAP8942466.1"
                     /translation="MLIEKVLNNNVVITRDERNQEMIVMGKGLAFKKKVGDPIFDSCI
                     DKKFQLTNDGLSQQFQELAQAIPLEYLEISCEIIEYTKEALQVQLNDSVYIALTDHLY
                     TAIERHKMGIFVPNVLLYDIKQLFPKEYAIGKKTVKKISDKYHIKLSEDEAGFIALHL
                     VNAQGIQSQQDNTYLFTITIQDILNIVRYYYKITFDEDSVHFFRFVTHLRFFLTRVLD
                     GEKSEVGEVSEELFQVIQKKYKDTFGCVEKIADFLAETFNYQMSTDEKMYLTIHTARL
                     VHENRCK"
     gene            47784..49535
                     /locus_tag="IV334_000369"
     CDS             47784..49535
                     /locus_tag="IV334_000369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP8942467.1"
                     /translation="MAKPSTNKLEGIEMGKYQELARKIVENVGGKENINGLTHCITRL
                     RFKLKNEEKANDEILKNMDGIVTVMRAGGQYQVVIGNHVPVVFEEVIKAGNLTFDEAV
                     STEKMRPFDMLIDGISGCFQPFLAILAAGGMIRGLTAFLVFLGAFDRGSGTFVMFDNI
                     GDSVFQFMPVIIGLTAARKFKVNEFVGMLLGAALMNPSLSLEALSGAAEAPLTTIFSG
                     TIFEAPIYQTVFGIPWIARNYASSVIPIIFIVLLASQIQKPIKKLVPEMIANFFVPFF
                     TVLITMPLGFLLVGPVFTFATDILMAGFETLLALSPVIYGAIVGFFWQILVMFGLHWA
                     IVPMGLMQFSVNGWQNIMTPVAVVSFGQTAALTALYFKLRNPKDKAIAIPAIVSGIVG
                     ITEPAIYGFTLPRKKIFIFTCVGGALGGAYSGLMNLTSWNQGGLGIFTIPNYIRPDGD
                     LTDVINVLIGIAIAMVVSFTLTFFFWKDEAGETDDIQKKSGKEIVKTPIQGQIAPLNA
                     AKDAAFAQGTLGRGILIYPEKGEVRAPFDGTVMTLFPTKQAIGMVSETGLELLIHVGL
                     DTVQLEGKYFESLVQQG"
     gene            49539..49709
                     /locus_tag="IV334_000370"
     CDS             49539..49709
                     /locus_tag="IV334_000370"
                     /inference="COORDINATES: protein motif:HMM:NF012578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8942468.1"
                     /translation="MKQGDLLLRFDKKKIEKEGYSLETPVIVTNYTDYLDILENQGET
                     VGPDDALITALT"
     gene            complement(49749..50651)
                     /locus_tag="IV334_000371"
     CDS             complement(49749..50651)
                     /locus_tag="IV334_000371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HAP8942469.1"
                     /translation="MLLREKIKEISFSPSENEVIQFLLSDSPALGEMTIQAIAERCYV
                     HPSTLIRVAKKMGFDGWVSFRNAFLAETAYLEGNFQDVDANLPFSTTDGIMTIANKMA
                     SLEQMTIHDTLSLIHHDQLQKAKQLMLQAKKIVIFASNSNTLISQDFMLKMKRIKMDV
                     TIVTTFGESFYEAYNCTSDMCGILISYTGENNVILRTAEILKQNHVPILALTSIGDST
                     LSAMSTAALRLTTRERLYSKIGNFTINTSICYLLDVLYSCVFAENYQQNLVHLISVGQ
                     KFDKRPISSAIMKEPFNFENKEVE"
     gene            50882..52285
                     /locus_tag="IV334_000372"
     CDS             50882..52285
                     /locus_tag="IV334_000372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HAP8942470.1"
                     /translation="MAKGIKIATIGGGSSYTPELMEGFIKRYEELPIREIWLVDVEEG
                     KEKLEIVSQMSQRMWDASPYEVKIYATLDREEALKDADFVTTQFRVGQLDARIKDERI
                     PSYYGMIGQETNGAGGIFKAFRTVPVVLAIVEDMKRLCPNAWLINFANPSGMVTEAVI
                     RYGKWDKVIGLCNVPVMAMMTEPAMLGKKKEELIYKFAGLNHFHWHKVADNKGNDRTA
                     ELIDRLYDEENGLPKNIFDIPFFKEQLKQMNMIPCGYHRYYYREEEMLEHALEEYQTI
                     GTRAQQVKETEAELFELYKDPELDHKPEQLQKRGGAYYSDAACETIASIYGNKETQIV
                     VSTKNDGAVPDLPADCVVEVTAYVGAQGARNVAFGSLPAAERGWLQVMKNMELLTIEA
                     AVTGDYGTALQAFTINPLIRSGKTAKRIMDELFIAHQQYLPQFKETISRLKEEGIEVQ
                     DELARELSGNTHIMEEV"
     gene            52285..53718
                     /gene="ascB"
                     /locus_tag="IV334_000373"
     CDS             52285..53718
                     /gene="ascB"
                     /locus_tag="IV334_000373"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HAP8942471.1"
                     /translation="MGFRKDFLWGGATAANQCEGGYDEGGRGLANVDVVPIGKDRFPV
                     ITGKKKMFEFDEDHFYPAKIAIDMYHRYKEDIALFAEMGFKTYRMSIAWSRIFPMGDE
                     KEPNEAGLKFYEDAFKECQKHGIEPLVTITHFDCPMHLIKEYGAWRSRELVGFYENLC
                     RVIFNRYKGLVKYWLTFNEINMILHAPFMGAGLYFEKGENEEQIKYQAAHHELVASAI
                     ATKIAHEVDPENKVGCMLAAGAYYPYTCNPEDVWASRKSDRENYFFIDVQSRGEYPAY
                     ALKEMERNGIQIEMEPGDAALLKEHTVDFISFSYYASRVSTTDNELLEQTAGNIFASV
                     KNPYLQASEWGWQIDPLGLRITMNDIYDRYQKPLFIVENGLGAVDTPDENGYVEDDYR
                     IDYLAAHIQAMKDAVELDGVDLLGYTTWGCIDLVSAGTGEMKKRYGFIYVDRDNQGNG
                     TLARSKKKSFNWYKKVIDSNGENLSND"
     gene            53863..54789
                     /gene="trxB"
                     /locus_tag="IV334_000374"
     CDS             53863..54789
                     /gene="trxB"
                     /locus_tag="IV334_000374"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HAP8942472.1"
                     /translation="MYDVIVIGAGPAGMTAALYASRSNLSVLMIEQGAPGGQMNNTAE
                     IENYPGFDSIMGPDLAYKMYESVSRFGTENAYGIVQDIKDHGSHKEVICEDKSYEGKT
                     VIIATGCIHRKLGVPGEEEFAGRGVSYCAVCDGAFFRNKRLVVIGGGDSAVEEAIYLT
                     QFASEVVIVHRRDELRAQKIIQDRAFANEKISFMWDTVVDEIVGNEMVVTGVKARNVK
                     TGNEFDLAADGVFIYVGLDPLTAPFREAGLTNEAGWIPTDNEMKTNMPGVFAIGDVRE
                     KDLRQITTAVGDGGIAGQQVYKYLESLEETTA"
     gene            55003..56730
                     /locus_tag="IV334_000375"
     CDS             55003..56730
                     /locus_tag="IV334_000375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HAP8942473.1"
                     /translation="MSWEQVYEQWVHEENLEENLKKQLNDLGEDPEKLEDAFYAPLEF
                     GTAGMRGILGPGINRMNIYTVRQATEGLARFMDTQGEETKRRGVAIAYDSRHQSPEFA
                     MEAAKTLAHHNIPSYVFESLRPTPELSFAVRHLKTFAGIMVTASHNPAAYNGYKVYGE
                     DGGQMPPADADALTKYVREVSNPLKVEVLSDEEAKHSGLITIIGEEVDAAYLEEIKAV
                     TIDRELVETMGKDLKLVYTPLHGTGKMLGERALKQAGFEQFVLVPEQAVADPDFTTVK
                     SPNPEEHSAFEYAIRLGEKEGADLLIATDPDADRLGAAVRLPDGSYEVLTGNQIGALF
                     AQYILEAHQSRGTLPENAAVLKSIVSSELPTAIAEHYGATMFNVLTGFKFIAEKIQQF
                     EEDHSHTFMFGFEESYGYLVKPFVRDKDAIQALLLLAEVAAYYKKQGKTLYDGLQTIF
                     ETYGYFAEKTISVTMSGQEGSAKISALMEKFRKEAPTEFAGAKVIQTEDFKELTRTDR
                     EGNVEKLTTPPSDVLKYTLEENGWIAIRPSGTEPKIKFYIGVKGSSTEDAAEKVASYE
                     AIIKKMTEE"
     gene            56802..57095
                     /locus_tag="IV334_000376"
     CDS             56802..57095
                     /locus_tag="IV334_000376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HAP8942474.1"
                     /translation="MPDTQKSKVEKVSQLFKMLSDPTRLKILLYLKDGEQNVTAITQA
                     VEMEQSAVSHQLRLLRENHVVKSHREGKAILYSLDDSHVLDILNQTLKHVEHQ"
     gene            complement(57187..58047)
                     /locus_tag="IV334_000377"
     CDS             complement(57187..58047)
                     /locus_tag="IV334_000377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase/pyrophosphorylase"
                     /protein_id="HAP8942475.1"
                     /translation="MSSDSTEIVTFFVISDSAGETATKLAQATMAQYPTVEFNLFRRT
                     FVTDEKTLRQALQDALEEKALVLHTLINQELIEITRSFCEEHQLFSFDVMTPPISEIE
                     RLTGIKPIRQPGALHLLNENYFKRIKAMEFAVKYDDGKDPRGFLEADVVLLGVSRTSK
                     TPLSLFLANKNLKVANLPLIPQAHIPKQLWEIDPKKIVGLTNNPDILNNIRKERMRSY
                     GLNPDTAYSDIEKIRAELDFANDLYEKLGCVVINVASLSIEETASLILNALDLEDHSY
                     YGTETTEKSD"
     gene            58139..58567
                     /locus_tag="IV334_000378"
     CDS             58139..58567
                     /locus_tag="IV334_000378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HAP8942476.1"
                     /translation="MKKQTLIEQSLETLKNNGFKYTKKREALLTYLVKRNRYVSAKEV
                     FDYMNQEFKGVSYDTIYRNLHDFAEIDLLEETDLNGEMKFRFHCCQGEIGQHHHFICT
                     VCGTTKEIHMCPMNYFEEQLGGCTIEGHRFEIFGRCENCQ"
     gene            58729..58905
                     /locus_tag="IV334_000379"
     CDS             58729..58905
                     /locus_tag="IV334_000379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010754017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HAP8942477.1"
                     /translation="MSKTVVRKNESLDDALRRFKRSVSKAGTLQESRKREFYEKPSVR
                     RKKKSEAARKRKKF"
     gene            58932..59378
                     /locus_tag="IV334_000380"
     CDS             58932..59378
                     /locus_tag="IV334_000380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HAP8942478.1"
                     /translation="MSLLSRLNDDMKAAMKAKDKESLQVIRMIKSSIQNEQIKEGHDL
                     TEEEELTVLSREMKQRRDSLHEFEEAGRDDLAEKVKSEIVIVEKYMPEQLSDEEIRQL
                     VQEAITQTGASSMKEFGKVMGAIMPKVKGKADGNQVNAIVKELLQS"
     gene            complement(59532..60911)
                     /gene="brnQ"
                     /locus_tag="IV334_000381"
     CDS             complement(59532..60911)
                     /gene="brnQ"
                     /locus_tag="IV334_000381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HAP8942479.1"
                     /translation="MEETKTYSWKQLLMVSSLIFGMFFGSGNLIFPVHLGQMAGSNWI
                     SAGVGFAISGALFPLLAILAVVVTKSDGLYDLAKPVGKWYAALFLILVHLTIGPFFGT
                     PRTAATAFEMAAKPFLPEKYDSLGMFLFSALFFLLAYFLTVHPTRLVKYVGKYLNSLF
                     LALLAIIFVVSLFQPMGNLNQSAADSYQTNALTNGVLEGYNTVDAVALLALSVTFVHA
                     VRDLGFKDKKVTELTAKAGSLSVALEVMIYFGLVLLGAMSLNKMPLSENGGTALSQIT
                     NFYLGNFGASFLGVMVTLGVFTTAMGLVVSFAQDFHKLFPKVSYMTWLRLTTFVSFVV
                     ANAGLDNIIQWSLPVLMLLYPLSLALILLSLTAKFFQKSPFVYQVTMFFSAVPAVLDM
                     LANSPALVSQQTVVASLLGFYHHHVPFAALGLGWMVPTLLGYAGSLLFYYAYRLSGYK
                     QEANELPEE"
     gene            61617..62588
                     /locus_tag="IV334_000382"
     CDS             61617..62588
                     /locus_tag="IV334_000382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HAP8942480.1"
                     /translation="MTEESSSLEIRLTAADDVSMLLGAHDKHIKLIEETTETTIHTRG
                     EIIQIIGEQSQISLAASVIRTLQELIKRGIHVSTPDVVTALKMGQKGTLDYFVEMYEE
                     EIVKDRNGKPIRVKNSGQKKYVESVAKHDIVFGVGPAGTGKTFLAVVLAIAALKKGEV
                     QKIILTRPAVEAGENLGFLPGDLKEKVDPYLRPVYDALYQIFGLDHTNRLMERGVIEI
                     APLAYMRGRTLDDAFVILDEAQNTTVAQMKMFLTRLGYQSKMIVNGDTSQIDLPRGTT
                     SGLVHAERTLKQIKKIDFVNFEASDVVRHPVVAEIIKAYEKADLHQE"
     gene            62612..64804
                     /locus_tag="IV334_000383"
     CDS             62612..64804
                     /locus_tag="IV334_000383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDIG domain-containing protein"
                     /protein_id="HAP8942481.1"
                     /translation="MLNKTLQKLQEKLGKAALPFLLFLFFIIICGVTFSSVKQKANDF
                     REGQVAEESIRANKTIENTEETEQKRKLAAEAVTPEYTYQKDLADDQNNRIKQLFELI
                     DKTNDAINKSYDEKIDKAKEDENIPKPTTEERIAALKKNFENVNAENVAFYQKLPNNF
                     YSTIFEMTETEIHTVRDESLKLIDEQMSKQVRESELEAFKQEAEEQIQYLNVTPAQQQ
                     MIRYLVDQGIVVNDVLNEKKTEELKQSAREAVQPVMIFQGEIIVREGNQIDASAMKKL
                     ELLGLTNQTTSIFPLIAMVLAVLLQIGVLIYNSLQFQNAGERTKYVLFYVTAMSVSIV
                     LMKFFQLFQTEQATYIPLFYPAAFAPLVLSFFLNRRAGIMAGIFQAVSALFIFYDSIG
                     TNFLTIILMSYVFSGLMATIVRRKRISEQGLSAAMWVIIFPVMMDVILIVYQGMSFGD
                     ATTWLMLVCGFTGALFSFLLTIGLHPYIELMVNDDSVIVLNELSNPNHPLLKQLLEEA
                     PGTYHHSMMVANLSANAVAEIGGRSLLTRVACYYHDIGKIKHASFFVENLPSGAENPH
                     NFLLPEDSKHIIFGHVTDGAKILEDYNMPQMVIDICKQHHGTTLMRYFYIKAKERNPE
                     VTEEQFRYPGPKPQSREAGIVNIADSCEAAVRAMDHPTSEKIESFVHNLIEERISDGQ
                     LDDSGLTLKEIRKVEKSLISGLSSTFHSRIKYPKMKTEVEKIKEEQEE"
     gene            64821..65297
                     /gene="ybeY"
                     /locus_tag="IV334_000384"
     CDS             64821..65297
                     /gene="ybeY"
                     /locus_tag="IV334_000384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HAP8942482.1"
                     /translation="MDITFMDETNQVSEEKMKEIDDLLQFAASYLKLPEETEMSVTFM
                     DNAAIQVINRDYRGKDAPTDVISFALEEEGEDEIPVIFEDGENPLPRVLGDIMISIER
                     AAEQAEAYGHSYDRELGFLAVHGFLHINGYDHMTPEDEKEMFGLQKEILDAYGLKR"
     gene            65275..65676
                     /locus_tag="IV334_000385"
     CDS             65275..65676
                     /locus_tag="IV334_000385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HAP8942483.1"
                     /translation="MPMDSKDKNVEKNKHFLMSVEFAVQGVKTVFEEERNMRKHVAFG
                     ILALFMGVIFQLDRIEWLWLLLAVFLVWIVEIINTVFENVVDMFTDFHFHPIGKKIKD
                     MAAGAVLVTACFAVIIGLILFVPKIYHLLFN"
     gene            65691..66590
                     /locus_tag="IV334_000386"
     CDS             65691..66590
                     /locus_tag="IV334_000386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HAP8942484.1"
                     /translation="MPEHKSGFVAIVGRPNVGKSTLLNRIVGQKIAIMSDKAQTTRNK
                     IQGVYTTPEAQLIFIDTPGIHKPKHRLGDFMVEAAYSALKEVDAVLFMISADQKRGRG
                     DDFIIERLKNVHSPVYLIINKIDKIHPDELLGIIEDYSTQMPFAQVIPISATEGNNVE
                     RLMDVLVSEMPEGPQYFPDDQVTDHPEYFIVSELIREKVLFLTRDEVPHSVAVVVDSM
                     KRNENDKIHIQATIIVERDSQKGIIIGKGGKMLKQVGTKARKDIENLLGDKVFLELWV
                     KVQKDWRDKKVYLQDFGYRKDEY"
     gene            66733..67545
                     /gene="recO"
                     /locus_tag="IV334_000387"
     CDS             66733..67545
                     /gene="recO"
                     /locus_tag="IV334_000387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HAP8942485.1"
                     /translation="MSQLAESKGLILSSRNYKEKDKLVKIFTESSGKMMFYIKGIHRQ
                     NQPLAPALLPFTEAVYIGNFRSEGLSFLSSVKDVHAFRNIQEDIFLSGYGTYLLNLVD
                     AAIEDHQYDPHLFQFTQQALQRMDQGDDAEIITNIFEVQILQRFGIAPIWTHCVVCGE
                     TKGKFDYSSKSGGVICEKHWPMDEHRYHADPRAVYFVRMFSAISYDKISGIHLKEETK
                     QAIRDLIDQLYDEYVGIHLKSKKFIDQMKSWAHVLKTPEKNMPEKEIPEKEE"
BASE COUNT        22164 a        11846 c        14499 g        19313 t
ORIGIN      
        1 ttttgtctca gtcccttttt ttgcatattt ttagcattta tttcctaaaa aatgtaaggg
       61 gattcctata tttttaagta tacttgtttt tccttttata cattttatgg tattatcatt
      121 ttattagtta ttataaaaca atcgttagaa aggacgtcat tatgcaagta tacggaattg
      181 atgcggtgct tcgaatcgtg agtcatttgg cgtttatcta cttagccttc tggtctttac
      241 gttcgttacg cattgaaact tttttcagac caatgcataa tacgcaaatt agaatggcaa
      301 tcgtactttt ttcaattttt ttagggtaca ctgcaagtaa ttttttctta gaagttatcg
      361 ctttatgccg aaatcttttc gttagcctgt aaattttaaa aaaaggtact attttaaaag
      421 caaggatgtt ataatagaaa agattttgag taagtgtatg ctcattggaa ttttttggag
      481 ggaacaacat ggaagagatc atcgtaagag gtggcaatca attaaatgga acagtaagaa
      541 ttgaaggagc taaaaatgct gttttgccta ttttagctgc tagtttgtta gcagaagaag
      601 gtattacgac attagacaac gttccgattc tttctgatgt cttcactatg aatcaagtga
      661 ttcgtcactt aaacgtagat gtagattttg atgaacaaaa gaatcaagta acaatcgatg
      721 cttcaagaca attagaaatc gaagcgcctt atgagtatgt tagtcaaatg cgtgcttcaa
      781 tcgttgtgat gggtccgcta cttgccagaa atggacatgc aaaagtagct atgcctggtg
      841 gatgtgcgat tggtaaacga ccaatcgatt tgcatttaaa aggatttcaa gctttaggag
      901 caaaaattat tcaaaaaaac ggctatatcg aagcaattgc ggatgaattg atcggtaaca
      961 cgatttattt agatttccct agtgtaggtg ctactcaaaa tatcatgatg gctgccgtaa
     1021 aagcaaaagg aacaaccatc attgaaaacg tagctcgcga accagaaatc gttgatttag
     1081 caaacatttt gaataaaatg ggcgctcaag tctatggagc tggtactgaa acaatgcgta
     1141 ttgaaggcgt ggatcattta catgcagtca accactcaat cgtacaagat cgtatcgaag
     1201 caggtacctt tatggttgct gctgcaatga cacaaggcaa tgtgctgatt gcagatgcta
     1261 tctctgaaca taaccgccca ttgatttcta aattgatcga aatgggtgca gaaatcattg
     1321 aagaagaagg cggagtccgt gtcatcggac caaaacatat cttacctaca gatgtaaaaa
     1381 cgatgcctca tcctggtttc cctacagata tgcaagctca aatgacagct atccaattag
     1441 ttgcagaagg tacaagtgta gtgactgaaa cagtgtttga aaatcgtttc caacatttag
     1501 aagagatgcg ccgtatgaac gctcatgtca aaatcgacgg aaatgtagca atcatggatg
     1561 gaaaccatga attgcaaggt gcagaagtat atgcaacaga cttgcgggca gcagcagcat
     1621 tagttttagc tggcttgaaa gcaaatggaa tcacacgtgt gcgtaacttg aattatctag
     1681 accgtggata ttataatttc catatcaaac tacagcaatt aggtgcagac gttgaacgtg
     1741 tagacatgga tcaaacttct gctgaaaaaa cagcacaaac gattgcttaa ggaagtggct
     1801 gaatgagtaa ccaacgatat atcttattaa ctttgataaa gattttagtg gtgatcttat
     1861 tgcttatcct tctttttgtg gccggaacga tgatcggata tggtgtcatc ggtggcggca
     1921 atccatttaa agtattccag ccaagtttgt ggattcatat tcgagacttt tttcattgat
     1981 aaataagaat cacagtggtt ctagtcgata tgaagtgagc catgaagcag aacatgacaa
     2041 aagaaaacag aagggaacgt tgacgttagt cgcgatccct ttttttagta aaagaaaaga
     2101 aggaatctaa ttgatgaaga agatattgat ttatttagtt agaggatatc agcgctttat
     2161 ttcgcctgca tttccaccta gctgccgtta ttacccaacc tgttccaatt atatgataaa
     2221 agcaattcaa acccatggtg cactaaaagg gacggtaatg gggactgcac ggatcttacg
     2281 ctgtcatcct tttgtcaaag gcggtatcga ttatgtacca ttgacattcc aattaacgcg
     2341 aaataagaaa gataaaaaag aaccagatta taaaaaggtt gtgaaaaata cgagtagcac
     2401 tgagcgataa agaaaaagag gaaaattatt gtcaaagtaa caactccgca actacttata
     2461 tgggggacaa aaaatgatcg aagaactaaa caagaaagat ttaccatggt ccttgttatt
     2521 agatgcagat cctgataaag aaaaagttca agtatatgtt tccagaggga gtggctggat
     2581 atggaaagaa aaagaaaaaa caataggtgt actgatctat gtggcaaggg aaaaagaata
     2641 cgaaatcgtc aatgtagcag tagccccaac gtatcaagga aaggggattg gcggaaaatt
     2701 acttgagaca gcttttcaga agctttcaca actcgcttcc agtcaaactc agatcatcat
     2761 acgaaccggc agtactagca gtgcggcgtt acatctctat caaaaaatgg gctatgtgga
     2821 gattggtcga gtcaaagact attttatcca tcattactct gagcccattt ttgaaaatgg
     2881 agatcaatta agggatcaag taattttagc aattcactta cctaaaaata gctttaacta
     2941 ataaactttc aattcgagaa aatgctttca aatgctaaat agatacatta caatagcttt
     3001 agcaaacaaa aaggagaata tttttatggg aatattagtt tttgattttg gtggatcggc
     3061 tgtcaaatat ggttgttggg atggcaaaga gattaaaggt aagggacaat ttgcaacgcc
     3121 tgaatcttgg gaagaaatga aagcgcaatt atttcaagtt tataaaaaaa tactcgtttc
     3181 ttttgaagtc gaaggaattg gaatcagctc tcctggagta gtgaataatg agaaacaggt
     3241 gattgaggga atcagtgcga ttccttatat tcatggattt aatattttcc gagatttgga
     3301 aactctgttt caactcccag taacgattga aaatgacgcc aactgtgccg gaatggctga
     3361 attttatgaa ggtgctgccc gtgattatca agatgttgct tttgttgtgg caggaactgg
     3421 gattggcggg gcgttgttca cgaaaggaca aatcaataaa ggggcacatc tttatggtgg
     3481 agaatttggg ttgatgttct taaatggcga taagacattc agtaaacttg gtacagcggt
     3541 acagatggct tggcgttatt gtgaacgcaa aggtctcgat aaaaattctt ataccggaaa
     3601 agatgtcttt gaattagcag aaactggaga tgcaatagca aaagaagaag ttgagagttt
     3661 ttatacgtat cttacaaagg gattattcag tattcaattt tcgtttgatc cagaagtgat
     3721 cgtattaggc ggcggtgttt ctgcaaaaga agggctcatt gatgagatca aaagccgaat
     3781 gaagaagctg acagaacaat tcgatctgca tgatttcgaa ccgcagattt tattatgtga
     3841 atacagaaac gatgctaatc ttgtaggtgc agcagcaaac tatctcgctg tacgtcaagg
     3901 ggagaattaa gagctttaga gaaggtgcag cttgcataaa agaagtgaat gaaatgaata
     3961 ggagattttt acctaaggaa tgttttattt gctgcagtat gataaaatat ctgagaggtg
     4021 aaaatgatgt caaaaaaaaa taaagatgtt gaagtgcgta tagaagaagt gaaaaaagat
     4081 atcaaaggta agaattatga agtcactcag ttatttatag ggaaaaaaat gattggtgag
     4141 atcttagcct atgggccaaa agaatatgaa attttctttg gtgaagaaga ttttggaaaa
     4201 gaaaaaagtc ttgaaaatgc gatagaaaca gtgattcgac actggaatct gcacgaataa
     4261 aaaaatatca aaaaaacttt caataaaggg ttgcaaaaat ctggaaaatt aggtattata
     4321 actaagtcag gttgatacaa acctttcgga ggagtagcga agtggctaaa cgcgacggac
     4381 tgtaaatccg ttccttaggg ttcagtggtt cgaatccact ctcctccatt tcattggggt
     4441 atagccaagc ggtaaggcaa gggactttga ctccctcatg cgttggttcg aatccagcta
     4501 ccccagtata ccacaatcgt aagctctcgt tagttcgaga gctctttttt ttgctttgct
     4561 accttgttcc ttgaagattc tgctgattta agatagagtt aagttaacta caagaattgg
     4621 agggatccac atgaaatgga aagaaaaagt agacgaagca gctgaaaagt tatatgattt
     4681 ggtcaaatca gaaaaataca atattgaagt gaatattccg aaaaaaggcg gaaaagcggt
     4741 ccaggtaaaa tcaaaacgcc caacgaatca tacaaaaaaa tggatggcta aaaaccgcta
     4801 ataataacaa aagatccata agtcggcatt ttcatcgatt ttagggtctt ttgttatttt
     4861 ctaggttgaa catatctaaa aaattgtttc tgacaatagg agtaggaaga acgatgtgtg
     4921 tctaaatcct ctgttgatgg atagaaacct ttatcttttt cagttgtgaa tcaggtatat
     4981 accagttata atagaagaaa cgaaaggacg ggtgtacgtt atgtcgaatc aattacctgt
     5041 atatatacag attcatgacc aattaaaaaa tgaaatcgaa caaggtatat ggaaagtcgg
     5101 agaccgtctt ccttcagaaa gagaattagc agtgaaattc aatgtcagcc gtatgacgtt
     5161 gagacaagca attcaaaact tggcagatga aggaatctta gaaagaaaga tcggttcggg
     5221 tacatatgtt gcgcgagaaa aagtacaaga aaaaatgtct ggtgcaacaa gttttacaga
     5281 aattatggaa tcgcaaaatc gagtaccgtc tagtcggaca atctcttatt tcttgacttc
     5341 gccaagctca agtgaaatgg aaaaactaaa cttgagtaaa gatgacacga tcttgcgaat
     5401 ggaacgtatt cgtttcgcag atgatgtccc tatttgtttt gaagttgcca gtgtgccaca
     5461 aaagctgatc gatggctaca gcaaagcaga gattactgaa tcgttgtatc gcacgctaga
     5521 agaaaaaggc gggaaaaaga tcggtccagc gaatcaaacg attactgctg tgttggcttc
     5581 tgagaaaatt gctgactatt tagaggtaaa acgaggagat gcagttcttc gtttgcgcca
     5641 gatttcttat tttgaagacg gtacgccgtt tgagtatgtg cgtacgcaat acgtaggaaa
     5701 tcgttttgag ttttatttgg aaaaataaaa aacaggtgat ccctgattcc ttacttagaa
     5761 ataaaagaga atcagaatca cctgttttta tatatgatca gttaccagtt gctcgtgata
     5821 agaaacagga ccctcttcaa attggacttg tccgttcagc agctcaatga tggattcttt
     5881 aaagtggtca gcactttttt catctacagc acagatgacg ctaacttgat cagtatattg
     5941 gatttctttt ataaaaaaat gttggagttc aagataattc tgtaattttc ctagttgcgg
     6001 atacgtgatc atcacagaga tctcctgttg caaggtacct tctacgattc caatgtcaga
     6061 caatgcttga gagacagcat gagtgtatgc tcgtattaat ccgcctgtgc cgagtttcgt
     6121 tcctccaaaa tatcgagtga ctactgcagc tacattgatc agttgatttt ttttgaggac
     6181 ttccaacatc ggtacgccag ccgttccact tggttctcca tcatcgctgc ttcgctggat
     6241 ttcatttttt tctccaatga tgaaggcgct gcagttatgg ttcgctttcc aatgttcttt
     6301 ttttatttct tgaatgaaac tttttgcttg gtcctctgat tctattcgtc taagagaaca
     6361 gataaagcgt gattttttga tctcaatctc actttgtcca ttttctttta ttgtacgata
     6421 gcgctcgatc atcttgtcac ctcttttttt cattttcttt attcagtata aataaagttc
     6481 taggttgttt caacactgta acggttgcgc aatcgctgta gtatgcgata ataaagacaa
     6541 aggtaggtgg atctggggat gcaggaacga atgaaaaagt acgatgcaat tacgcattat
     6601 ttgaagaaca atggcggctc tcaagtaacg ttgaccttta cccaatttga cgaactgctg
     6661 tttcccagta acggacttcc caaaacagca agggaaagta cagattggtg ggcaaatgat
     6721 tataaacacc ctgaaaaagg tgcctatggc tggataaatg caggctatga agtagttgtc
     6781 atcaatttgg acaaagaata tgtcgtgttc aataaattag tgaaatctag ttggctattt
     6841 gattaaataa accgcgttta gaaaacgttg acaaatttct ttctttttat ataatagagt
     6901 aacagacgcg aagaaaacgt ttgcgtagtt tgttttaaaa aggagagaaa agtatggcta
     6961 aaaaaacaat catgcttgta tgttcggctg gtatgagcac aagtttacta gtaacaaaaa
     7021 tgcaaaaagc tgctgaagca aaaggaatcg actcggatat tttcgctgtg tccgcttctg
     7081 acgcagataa caatcttgca aataaagatg tagatgtact gttattagga cctcaagttc
     7141 gctttatgaa agctgatttt gaaaaacgtc tggaaccaaa aggcattcct ttagacgtca
     7201 tcaatatggc tgattatggt atgatgaatg gcgaaaaagt gttagatcaa gcaatcacgc
     7261 tcataggtga ataaatagaa cagtataact atcgaacaag cgatccaaaa aatagatcgc
     7321 ttgttttttc gtatattttt ttagggcgaa taatatgaat catttttggg gtagaaggct
     7381 tctgacgaga gatattaaag caatagactg tgaggaggta aatttaccaa attatgagaa
     7441 gatcgcagca gtggaacgtt tgggcaactg gattctttgt catcgctgtg gaatgaggac
     7501 gtccgtcatt gatggacagc ttgcagatta cggttatttt tgtacacatt gtctatattt
     7561 agggcgatgc gacactcaac aagatttata tttgtttgat cagcctgaag caaaacctcg
     7621 agaagtcgtc tttttttgga caggacagct tactgaaaaa caaacggaaa tcgcaaagag
     7681 aatactttgc cattcagaaa aaagacatca tcttatttgg gctgtaacag gtgcagggaa
     7741 aacagagatg ctttatccta ttttagtgga aactttaaaa gctggaggaa gagtagcgat
     7801 ttgtacaccg cgagtcgatg tttgtaatga attgttttta cgctatcgtt atgtttttcc
     7861 agaagaaaaa atcacgttgc tccatgggaa tacagcaacg gcttatacct ttacttcttt
     7921 tgttatctgt acgattcatc agctataccg tttttatcgg tcctttgatt taatagtgat
     7981 cgatgagatc gatgcgtttc cttataacgg ggatgcagga ttagctcatg ctgtgcaaac
     8041 agcgattaaa ccagacggac gattcatcta cttaagtgcc acacctgata aaaagttact
     8101 aaaggaaatc aagcagacgt ttgacatcca caaacttgct ttgcgctttc atcggcgttt
     8161 acttcctgaa cccgtcttgg ttttcatgaa taattggcga caaaaatgca agaacaagaa
     8221 gaagataaaa tctttggtgc ggatcattca tcaattaatt aaagaaaacc atatcctttt
     8281 attttgtcca agtatcacga tgatgcatcg tttgaaagcc acacttcaag aggctcttcc
     8341 gagatatgag atcacagatg tttcttcaag agacaatcag cgggcggaaa aagtccagaa
     8401 aatgcgagaa gcaaaatatg acatactgtt aaccacgatg atccttgaac gtggagtcac
     8461 atttgaaaga atatccgtta tcgttttagg agcggatcat ccggttttta ctaaatcttc
     8521 tttggtacaa atcgcaggaa gagccgatcg taaagggcct tatacgaaca gtcaagtata
     8581 ctttttctat gaagaaaaaa ctgcagctat tcgaaaagct tgtcaggaaa tcaaagaaat
     8641 gaatgaagaa gggaagaaga tgctgtgagg tgcagttgct gcagtaaaga tatcgtccaa
     8701 aatttaacat tcagcgagat tattggtgtt tcgaaataca gattacctca gacttgttca
     8761 gtttgtcgcg cacaatttca tttattggaa acaagaggct gttctggctg tcaaaaacca
     8821 atccatttca atctagagag tacgaaaaag aaagtaaagt ctattcccgt ttactgcagt
     8881 gactgtcaag aatggaagaa gaaatatccg acctatgatt ttcagcaccg tgcctttttt
     8941 gtgtacgatg aaaatatgaa ggcctggctc aatcaataca agtttatggg agatatccga
     9001 ctagcaggca cctttgcata taaatggacg cagataaaaa aagaataccc gacatacatc
     9061 tattgtccaa tacctttatc tgaaaaaagg cgaacagaaa gaggattcaa tcaagtaagc
     9121 gagatgttga aagcagcaag aattcctttt cagatgctac tcaaaagaga gaagcatctg
     9181 ctggctcaag caaaaaacac gcgaaaggaa agattggaga tgcctcaacc ttttgaactt
     9241 gcagtcacct tttctaagat acaaaataaa aaaatattga ttattgatga tgtctataca
     9301 acaggacaga ctcttttcca cgcagcagat tgtttgcagg tcgcacagcc gaaaatcatc
     9361 cgaaccattt ctttagcaag atgaattaga tgtggaaatt tgatagcgat ttaatttgca
     9421 aataacctag agttcgatat aatagaatta agaagaggga aagagtggtc cttctccttc
     9481 ttgattgtgg tagacgaaag gggtaatttt tatgtttaga tataatgtac gcggcgaaaa
     9541 tatcgaagta actgaagcga ttcgcgacta cgttgagaaa aaagtaggca aactagaacg
     9601 ctatttcagt gacgcacccg atgcaacggc gcatgtgaac ttaaaagttt atacagaaaa
     9661 aacagcaaaa gtggaagtaa cgatcccatt accttatctt gtattacgtg ccgaagaaac
     9721 ttctccagat ttatacgcaa gtatcgattt ggttgttgac aaattggaac gccaaatccg
     9781 caaattcaaa acaaaaatta atcgtaaatc ccgtgaaacg ggcatgaata ctgcagatgc
     9841 tactgcttta ttcaatgaag aggacgaaaa cgaaaatgat tctgaattag atatcgtacg
     9901 tacgaaacgc ctttcattga aaccaatgga tagtgaagaa gcagtcttac aaatgaacat
     9961 gctgggacat aacttcttta tttttgaaga tgcagaaaca aatggtacaa gtatcgttta
    10021 ccgccgtaaa gatggaaaat acggactgat tgaaacagac tgatcagcct tacttaaata
    10081 acaacgaaaa cttgcagcag ctactgacag ctgccgcaag tttttttgag tttcctcctc
    10141 ttttagatga aatatgaata aattatgaag ttcttacttt aatacgctta agatatttca
    10201 attatctata actaatgata aaatgaaaag taaagtctac atagtctgga taatacacag
    10261 aaggaaaagg agattacaca acgcatggcc aatttgattc gttcacttat cgaaaacgat
    10321 aagaaagaac taaaacgttt agatagcatt gcaaaaaaag ttgaagcata cgctgaccaa
    10381 atggcagcat taactgatga acaattacag gcaaaaactg ctgaattcaa gggcagatac
    10441 caaaaaggag aaacattaga tcaattactt ccggaagctt ttgcagtcgt ccgagaagca
    10501 gcaaaacgtg tattggggtt atatccacac cacgtacaat tgatgggtgg aatcgtgctt
    10561 cacgatggaa atatccctga aatgcgtact ggtgaaggta aaaccttgac cgcaacgatg
    10621 ccagtatatc taaatgctct gactggcgaa ggggtacacg tagttaccgt caatgaatat
    10681 ttagcaaccc gtgactcgac ggaaatgggc gaactttata atttcttagg attaacagtg
    10741 gggttgaata tcaactcaaa atcttcggat gaaaaacgcg aggcttataa ttgtgatatc
    10801 acttatagta cgaataatga attaggattt gactacttga gagacaatat ggtcgtttac
    10861 cgccatcaaa tggtacaacg tccgctgaat tatgccattg tcgatgaagt tgactcaatc
    10921 ttgattgatg aagcgagaac gccgctgatc atttcagggc aagcagaaaa atccacagct
    10981 ttatatacaa gagcagacaa cttcgtgaaa cgtttgaaag aagaagaaga ttataaaatc
    11041 gatgtacaat caaaaacaat cagtttaacc gaagcaggga tcgaaaaagc agaagaaaca
    11101 ttcggtttgg ataacttata cgacattgaa aatactgcat tgacccatca cttggatcaa
    11161 gcattgagag ccaactatat catgctttta gatatcgatt atgttgtcca agacggaaaa
    11221 gtactgattg ttgaccagtt cactggtcgg atcatggatg gacgccgtta ttcagatgga
    11281 cttcaccaag caatcgaagc taaagaaggc gtagagatcg aagacgaaac gaaaaccatg
    11341 gcgacgatca ctttccagaa ctatttccgt atgtacaaga aacttgcggg aatgactggt
    11401 acagctaaaa cagaagaaga agaattcaga gaaatttata acatccaagt ataccagatt
    11461 ccgacaaatc gtccggttat ccgtgatgat cgtccagatt tgctttaccc aacattacaa
    11521 agcaagttcc acgctgtcgt gcaagatatc aaagatcgct accgcaaagg tcaacctgtg
    11581 ttagtcggaa ctgttgcggt tgaaacatct gagttattat cagatatgct gaatcaagaa
    11641 cgtattcctc atgaagtgct gaatgcgaaa aatcacttta aagaagcaga gatcatcatg
    11701 aatgctggtc aaaaaggtgc tgtgacgatt gctaccaaca tggctggtcg gggaacagat
    11761 atcaagttag gacttggggt aagagaactt ggtggtctag cagttatcgg gacagaacga
    11821 catgaatctc gtcgtatcga taaccagctt cgaggacgtg caggacgtca aggagaccct
    11881 ggtatgtcac agttctactt gtcattggaa gatgatttga tgaaacgatt cggttcagaa
    11941 cgaatcaaat ctttcttaga tcggatgaag atcggtgagg aagatgctgt tatccaaagc
    12001 aagatgctga cgaaacaagt agaatctgca caaaaacgtg tagaaggaaa taactatgac
    12061 acacgtaaaa acgtcttaca atacgatgat gtgatgcgtg aacaacgtga agtaatctat
    12121 aaacagcgac aagaagtaat catggaagaa aaaagcttat ccaagccact aatgaacatg
    12181 gtcaaacgga caatcagtcg tgtagtagat gcgcatacac aattagagaa agaaaattgg
    12241 aatttggaaa gtatcatcga ttttgccggc agtactcttg tccatgaaga tagtattact
    12301 cttgcagata tcgaaggaaa aacagctcag gagatcaaag agtatctaaa cgaacgagca
    12361 caagaagtat ttaataccaa agcggctcaa ctaaatggtc cagagcaact gttggaattt
    12421 gaaaaagtcg tgatcttgcg tgttgtggac tctaaatgga cagaccatat tgatgcaatg
    12481 gatcaattac gtcagtcaat cggcttacgt gcttatggac agaacaatcc attagtcgaa
    12541 tatcaaacag aaggctataa gatgtttgaa gatatggtag gcgccatcga gtatgaagtc
    12601 actcgaatct ttatgaaagc tgagatccgt caaaacgtac agcgtgaaca ggtttctcaa
    12661 ggaacagctt ctcactcaga agacggagat gccaaagaaa atagtaatac tagcgcgaaa
    12721 aaacagccaa tccatgtgaa caaggtcgga agaaatgatc cttgtccttg tggaagcggg
    12781 aaaaaatata agaactgtca tggaaaaaat gcaaactaat ttctgacagc aggattggtg
    12841 agacaaattc tttttttaag aattcgtctc accttctttc tgttatttgc tgattctgtt
    12901 agactagata taatgaaaaa agtatagtga aagaaggaaa aaacatggaa atatccgaaa
    12961 ttcgaaatct cctgacagaa atgcaggaaa aggtcacaag cttcaggagg tctctttgac
    13021 ttagaccagc tggaagaaga cattgcggaa gcagaagcaa aaatggctga acctggtttt
    13081 tgggatgata gcgaagctgc tcaaaaagtg atcagtgaaa acaatgcaaa taaagaaact
    13141 tatgatcaat tccattctct tgctgaagaa ctggaagaaa tcgaattgat ggtggagatg
    13201 ttgcaggaag aacctgatgc agccatgcaa gaagaagcag aaacacgtat caatgcgcta
    13261 gatgaaaaaa tgaagacata tgaattatct atgttgttag atggtcctta tgatcgaaac
    13321 aacgccatcg ttgaattgca tccaggggct ggcggaacgg agtcccaaga ttggggcagt
    13381 atgcttttac gtatgtatac acgctgggca gagcagcatg ggtatcaagt agagacacta
    13441 gattatcaag caggtgatga agcaggaatc aagagtgtga ccctactgat caaaggacac
    13501 aacgcatttg gttatctgaa atcagaaaaa ggtgttcatc gccttgtacg tatctcgcct
    13561 tttgactcag cgaaacgacg tcatacttct ttttgttcgg tagatgtgat gccagagctt
    13621 gatgaaacga tcgatataga aatcaatcca gatgatttga aaatcgatac gtaccgagca
    13681 agtggtgcag gtggtcagca catcaataaa acagaatctg ctgtacggat cactcacttg
    13741 cctacaggaa cagtagtagc tagtcaggca caacgttcac aattgaaaaa ccgtgagcaa
    13801 gcgatgggga tgttaaaagc taagctttat caattagaag tggaaaagaa agaacaagaa
    13861 gcagctgcac ttcgaggaga acaaaaagaa atcggctggg gttcacagat ccgttcttat
    13921 gttttccatc catattctat ggtaaaagac catcggacaa acgtagagac aggaaatgtc
    13981 caagcagtta tggacggtga ccttgattta tttattgacg gctatttaaa aatgcatcta
    14041 caataagaac ccattgttaa attgaaataa aattgtaaga ttttgcaagc atgttgaaac
    14101 attgccatgc tataatagct aaagactgaa aaagatatgg agagaacgcc atgattgaaa
    14161 tgaaggatgt aatgaagaag tactcaaatg gtacgaccgc tatccgcaat ctctcaattg
    14221 tcatcgaaca aggagagttt gtctatgtgg ttggtccgtc tggagccggt aaatctacat
    14281 tcatcaaatt gatgtatcga gaagaaaaag cttcaaaagg aactttgaac gtcgcaggtc
    14341 atgatctaat agcaatcaaa aaccgcgaag ttccttatct acgcagagaa atcggcgtgg
    14401 tgttccaaga ctataaatta ttaccaagaa aaacagtata tgaaaatgtt gcgtacgcta
    14461 tgcaagttat cggcagaaaa cctcgggata tcaaaaaacg agtaatggaa gtgctggacc
    14521 ttgttggttt gaaacacaaa gttcgagtct ttcctagcga attatccggt ggggaacagc
    14581 aacgtgtctc tattgctcgg gcaatagtga atacgcctaa agtacttatc gcggatgaac
    14641 cgacaggaaa tctggatcca gaaaactcat gggaaatcat gaaattatta gatcggatca
    14701 atgcccaagg aactactgtc gtgatggcta cacataacag tacgatcgta aatacgattc
    14761 ggcaccgagt aattgctatc gaaaacggcc gcattattcg agatcaagcg gaaggggaat
    14821 atggatacga tgattagaac ctttttccgc catttattag aaagtatcaa gagtttgaaa
    14881 cgtaacggct ggatgacagt tgcttcagtc agtgcggtaa cgatcacatt gactttagtc
    14941 ggtgtattca tggcagttat catgaacgca actaaattag ctcaagatat agaaggaaac
    15001 gttgatgtct ctgtattcgt agatatcggt acaaaagacg aagagatgaa aaagctagaa
    15061 aaagaattga atgacttaga tcatgttaaa aaagttgaat tttcaagcaa agaacaggaa
    15121 ttgaaaaaaa tccaaaacga aatgggtgat tcctggaaat tgtttgaagg tgacaataac
    15181 ccgttatatg atgtgtatgt agtaagtgct actgatcctt cttatacaaa acaagtagca
    15241 gaagaagcag cagatttgaa gaatgtttct cgttctgatt acggtggagc ttcctcagat
    15301 cggattttcc aaatcgctgg tgcagttcgg acatggggat tagttgcagc ggcattgctg
    15361 ctgttcgtag cgatgtttct catttcgaac acgattcgga tcacgatttt gtctagacag
    15421 cgtgaaattc aaatcatgcg tttagtcggt gcaaaaaatg gatatattcg ctggccgttt
    15481 ttccttgaag gtggctggat cggtctgatc ggtgcgattc ttccgatttt gatcatcaca
    15541 tttggttatg catggttttt ccaattgatc aatccatcat tattacgttc ccattattca
    15601 ttgattcatc cacaaaatat cgtttggaaa atcaatcttt taatggtcgg cattggtgtg
    15661 atcattggtt cacttggctc gatcatttct atgagacgtt tcttgaaatt ctaataccac
    15721 aatataaata aaatgagaaa gttggacagt ttcgttccag ctttcttttt ttattggcta
    15781 ggagaataga aggaagcggc ttttttccct ttactttaaa aaacaacttg ggtattctta
    15841 agttggtaga agaagaggtg aagccgtgaa aattatcgtt gacaattttt ttacgatcct
    15901 tttgattatg aatattttgc tttcctttat catcgttttt cgagaaagaa aggaaactgc
    15961 tcaaacatgg gcatgggtgc tcgttttgat gttcattcca gttgtaggat ttattttata
    16021 tattttttta ggaagaggga tttccaaaga gaaaatcttt gatttgaaga tccaagggaa
    16081 aatcggtaag aacctggaaa ttgaagaaga caaacaggca ttgatgcgag ggctttatcc
    16141 tcatccgcct actggacaag tagatgtgaa acaattgatc tatatgctca cggtatttga
    16201 aagtacgctc tatacgacta aaaatgaaat cactttattt acagatggtc gtgaaaaatt
    16261 cgatgcgctg attgaagata tcaagcaggc agaagaccat atccacttcc agtattatat
    16321 ttatcgcagt gatgcattag gtgaagaagt tcgagatgca ttgacagagg cggcaagacg
    16381 aggggtaaaa gtcagagtcc ttcttgatgc atggggttcc acacaagttt cttccagctt
    16441 ttttcagaat ttgaaaaaag caggcggaga agtcgcattt ttctttcctc tatttgttcc
    16501 gtatattaat cctcggatca attatcgcaa tcatcgtaaa atcgtagtta ttgatgggaa
    16561 aatcggatat acaggtgggt tcaatgttgg aaatgaatat ctaggattgg taaagaaatt
    16621 tggttattgg cgagacaatc atttgcgtat ttacggagaa gcggtgtata ccttgcaaaa
    16681 ccgttttttg atggactgga attctcagca tacaaaagaa gtaggctatt ctcccaaatt
    16741 ttttccgtcc attcactcta ctggtgatat agctgttcaa atcgtgacaa gcggaccaga
    16801 taccgaacat gaacaaatca aaatgactta tttgaaaatg atttcgttag ccaaacggga
    16861 gatattgatc caaacgcctt attacatacc ggatggatcg attcatgaag cactgaagtt
    16921 ggctcttctt tcaggcgtaa aagttcatat ccaaatacct aacaaaccag atcatcttct
    16981 cgtttactgg gcaacgtatt ctttcgctgc agagctaatc gagtatggag caaggatcga
    17041 aacatatgag aatggattta ttcatgcaaa gacgatgatt atcgatggtg gcattgtttc
    17101 agttggctca gcaaatatcg atgttcgttc atttcgcttg gactttgagg tcaatacact
    17161 ggtttatgat gaacgaatgg cttcacgtgt aagaaaagct ttttttgaag actcgaaaat
    17221 ttctacgcat ctcacaaaag aaatgtatga aaaccgagga ataatcatca agatgaaaga
    17281 aggattagca agattgattt cgccgctcct ttaaaacata aaaaggacat gagaaccatc
    17341 aacagatgga gatctcgtgt ccttttctaa cggggccatt tctgagagta cgtcagaaat
    17401 ggagaaagcg tctcgcatga gcgaaattaa ctttggggta tctcttgttt tttggggaaa
    17461 gcaagaaata agctaatata tgctattaaa agagacacat ttcaaagcca gtatagcata
    17521 gaaactttta atgagatagt tttttttaca attatgagaa tttattttgt tatttaatga
    17581 ttttgattcg ttttcatcaa gtaatgaaaa tgataaaata aacatacttt aggattaaag
    17641 gagatttcta atgaaaaaac ttaaattatt cttaccaata ataggattag ctattctttt
    17701 atcaggctgt tcaaagtgga ttgatcgagg agaatccatt acggctgttg gctcttctgc
    17761 tttacagcct ttagtagaaa cagttgctga aacttaccag acagaaaatc ctggaaaatt
    17821 catcaatgta caagggggcg gaagcggaac tggtttgtct caagtacagt caggagcagt
    17881 cgacatcgga aactctgatt tgtttgcgga agaaaaatcg gggatcgatg cctcagcatt
    17941 agtcgatcat cgagtagcag ttgttgggat cactcctatt gtgaacaagg atattggcgt
    18001 aaaagacatc agtatggaaa acctgaaaaa actatttaca ggcgagatca aaaactggag
    18061 tgaagtcggt ggaaaagatc agccagtagt tatattgaac cgcgcttcag gaagcggaac
    18121 ccgtagcacg tttgaaaaat gggtattgga cggagaaaca gctgttcagg cacaagaaca
    18181 agattcaagc ggaatggtgc gccagatcgt tgccgacaca cccggtgcaa tcagctatgt
    18241 tgctttttct tatgtttccg atgatgtaga tacgttgaat gttaacggtg tagaaccgac
    18301 agatgaaaat gtgacaacga acaaatggac gatttgggca tatgagcata tgtacacaag
    18361 agatcatcca aaaaaactga caaaagaatt tttagactat atcttatctg atgaagtaca
    18421 agataacatc gttggagaac taggttatat ccctgtttct catatgaaag tcgaacgtga
    18481 ttggcaagga aatatcgtta aagagtaatc tttacacaaa atttacaaac ttagctaaac
    18541 ttttacatgt tattaaaacc aaattgatgt tcaatttgta cactgagtga gtatgttaaa
    18601 cctgattaag ggggagtcat ctgtggaaga tgtgaaaaaa gtattattaa caaaatcaaa
    18661 acgttcaaaa atggaacagc gtggaaaatt catcagtttt ttatgtatcg cgctgatcgt
    18721 gttggttgta ttatctattt tttattttgt agctagtaaa ggattggcca ccttttttac
    18781 tgataagatc aatgtatttg attttctgtt tggtacagat tggaatccaa gccaaatcgg
    18841 agcaaatggg aaaccaatgg taggggcatt gcctatgatc acaggttcgt ttattgtgac
    18901 ctttttatct gctatcgttg ccacgccatt tgccattggt gcagcagtat tcatgacgga
    18961 gatctctcct aaaaacggaa cgaagatctt acagccggtc atcgagctgc ttgtaggtat
    19021 tccttctgtc gtttatggtt ttatcggttt atcagttatc gtgccttttg tcagaagtat
    19081 ttttggcggg actgggtttg gtatcttagc tggtacattt gtgttgttcg ttatgatctt
    19141 gccgactgtc acaacgatga ctgttgatgc attaaaggca gtgccacggc actatcggga
    19201 agcttcatta gcgcttggtg ccacaagatg gcaaacaatc tacaaagtcg tgctacgagc
    19261 agccgtgccc gggattttaa cagcagtcgt ttttggtatg gctcgtgcgt ttggtgaagc
    19321 cttagctatc caaatggtca tcggtaatgc agcattgatg ccaacaggtc ttactactcc
    19381 tgcttctaca ttgacgtcca tcttaacaat gggaatcgga aatacaatca tgggaacagt
    19441 tgaaaataat gtgttatggt cattagcgct gatcttgcta ttgatgtcat tattctttaa
    19501 tatcgtgatc cgtatgattg ggaagaaagg agagctgaaa taatgaacgc aaaacgctca
    19561 gataaaattg ctacaggtgt tttatatata gtttcgggga tcatcgtatt gattttagca
    19621 gctttactac tttatatctt agttcgggga ataccgcaca tttcatggtc cttcttgacc
    19681 gaaccttcca aagcctacca agaaggcggt ggaatcggga tccagctatt taactcgatt
    19741 tatttgcttt tgatcacgat gatcatcagt tttcctattt ctttaggtgc gggtatctat
    19801 ctttcagaat atgctggaaa aaactggcta acagatgtga ttcgcacgtc gattgaaatc
    19861 ttgagctcgt tgccttctgt cgttgtcggt ttattcggtt tcttggtttt cgttattcaa
    19921 ttccagtatg gtttctcgat tatttctggt gcattggctt tgactgtatt caacttgcct
    19981 cttttgacta gaacagttga ggagtcttta caagcggtcc attacacaca acgagaagca
    20041 ggattggcct taggtttgtc acgctgggaa acagtgataa aagtggtggt tccagaagcg
    20101 acaccaggga ttttgactgg tgtgatccta agttctggaa gaatttttgg tgaagcagct
    20161 gcattgatct atacggctgg acaaagtgca cctgcgttag attttacgaa ctggaatcca
    20221 ttaagtgttt caagcccgat cagcattttc cgtcaagctg aaacattagc tgtccatatt
    20281 tggaagatca atacagaagg cacgatgcct gatggtgttt ctgtttcggc tggcgcttct
    20341 gccgtgttga ttattgctgt tttactgttc aacttcggcg ctcgtgcgat tggcaaacga
    20401 ttataccgaa aaatgacttc agcctaagga gaagaagcaa aatgaaaaaa tataactgga
    20461 atgaaacaaa tgtcatcacc atgccaaaag agaaagaagt agcactatat acggatgatc
    20521 tacatgtctg gtatggcgac aatgaagcaa tcaaaggagt ggatctggaa tttgaaaaaa
    20581 ataagatcac tgcactgatt ggtccttccg gctgcggaaa atctacgtat cttcgttcgt
    20641 tgaatcgaat gaatgatggg atcgctaata cgaaaatcac tgggaaaatc atgtatcaag
    20701 gcgtagatat taatgcgcct caagtggatg tttatgagat gcgcaaacgg attggcatgg
    20761 tttttcaacg tcctaatccg ttcagcaagt cgatttatga caatattaca tttgctttga
    20821 aacagcacgg cgaaaaaaac aaaaagaaat tagatgaaat cgttgaaaca agcttgaaac
    20881 aagctgcctt gtgggaccaa gtaaaagaca gcttggacaa aagtgccttg gctttatctg
    20941 gcgggcaaca gcaacgttta tgtattgctc gtgcgatcgc gatgaaacct gatatcttgc
    21001 tgctagacga gccggcaagt gctttggacc ctatttcgac aggaacggtt gaagaaacac
    21061 ttgtaaactt gaaagaagac tatacgatca tcatcgtgac gcataacatg caacaagcag
    21121 ctcgtatcag cgactatact gcctttttct atttaggaaa agtaatggaa tatgacaaaa
    21181 ctaacaagat atttaccaga ccaaaaattc aggcaaccga agattatgtg tccggtcatt
    21241 ttggttaaga cggaggcctc aacatgacaa aagagattat ttcatctaaa gatcttcatc
    21301 tgtattatgg aaagaaagaa gcactaaaag ggatcgatct aagcttcaat caaggggaaa
    21361 tcactgcgat gatcggtcct tctggttgcg gaaaatccac ttatctgcgt tctttgaacc
    21421 ggatgaatga cctgattccg aatgtaacga tcaccggaag tgttttatat aaagacaaag
    21481 acatctatgg tccaaaaaca gatgttgttg aactaagaaa agaaatcggc atggtattcc
    21541 agcagccaaa cccatttcct ttttctatct atgaaaatgt catctatgga ttgaaattaa
    21601 aaggggaaaa agataaaaaa gtccttgatc aagtagttga agaaagcctg aaggcagcgt
    21661 ctgtttggga tgatgtgaaa gacaaattgc ataaaagtgc tttatcttta tccggtgggc
    21721 agcagcagcg tgtatgtatc gcacgtgttt tggcagttaa tccggaaatc atcttattgg
    21781 atgaaccaac aagtgcgctt gatcctgttt ctactgggaa aatcgaaagt atgctgttag
    21841 aattaagaga gcggtatacg atgatcatcg tgacccataa tatgtctcaa gcttcacgta
    21901 tttcagataa gacggcattc tttttagatg gtcacttgat cgaatacaat gatacgaaga
    21961 agatattcat gaatccagaa aaacaagaga cagaagatta tatttcaggt cgatttggat
    22021 aaggaggaaa cggaatgcta cgtacacaat ttgaagaaga attgctgaat ctccataatc
    22081 agttctacga aatggggatg atggtgagca gcgccgtgca caagtcagtt cgtgcataca
    22141 tcaaacacga taaaatctta gcccaagaag ttatcgaaaa cgatataaat atcaataaca
    22201 tggaaacacg cttggagaag aaaagttttg aaatgatcgc attgcaacag ccagttacaa
    22261 ctgatttgcg tatgatcatt acggtcatga aagctagctc ggatttagaa cgtatggccg
    22321 atcatgcggt gtctgttgct aaatccacga ttcgcgtaaa agggcagacg agaataccgg
    22381 aaatcgaaaa agagatctcc gatatgtccg attatgtgaa aaaaatggtg gacaacgtcc
    22441 tagttgctta tgtcaaaaca gacgaaaaag acgcacgtac gatcgccaat atggatcaaa
    22501 gagtcaatga ctatttcaat cgaatctata atgcaacaat caaaaatatg caggaaaatc
    22561 ctgagacagt catcagtgga acagactact tgaatgttgc cagttatcta gaacggatcg
    22621 gagactatgt gacaaacatc tgtgaatgga tcgtctactt ggctacaggg aaaatcactg
    22681 agttaaatac gaaccacaat gagattttct aaactgaagc tgtgattatg ccgtttgttt
    22741 aggaaaagag gttgtgaaat cagcggttgt gaaaaagctg ttctgcatca agtaataaga
    22801 acagtcaaac aaaaaatagc tcctcatatt tttcgtttgg ctggacttat taccgcagat
    22861 gcaagctttt gaacaccgtt tattcgggaa agggtttgtg acaaaacatg tcacaaaccc
    22921 ttttcatcat ttcatagaaa ggataaagaa tgcaaaaaaa aattagcatc atcactatat
    22981 tttttatttg tgtgcttact tcatgggtta ttcagcatca agccgcttta tatgaagtac
    23041 ctgtcatcga gatagaaaaa gtacgaataa aagaggatca tcaattgatt acaggaaaac
    23101 tgatgaacac ggatcaaaaa ggagaatcga tttatttatc ggttccttat caaaaaaatc
    23161 aaatagaaac agcaaatata aaagcgagac aaatgtatct agtccaaatg catggagatg
    23221 aatggcagct agtagcaaaa aaaaatgatg gctggctttt cttcttcaca agtatcttca
    23281 ttggcgccat gttgcttatt ggtggaaaac agggtctatt tgctttagga agtctcatca
    23341 tcaatatcgt tttactttta cttttgttgc tcatctttac taaaacagaa gggatttctc
    23401 tattaaatat atctatcctg ttcgtcgtta tagggatgat tattagcata ttggctttag
    23461 atggatggaa tagaagcagt ataatcaaaa tttcagctgc agttacgagt gtttttttag
    23521 cattttttat ttgtcttttg acaatgaatc attggaaaga ccaaggactg cgctatgagg
    23581 aactgaatta tctgacacga ccgtatcgtt ctgtttttct ttctagcgta ctgattggaa
    23641 cagctggagg aacactcgat accgttatca cagtcattgc gacattagaa gaattgacta
    23701 agcgacaacc agaaataaca gcgaaacagt tatggctttc aggaaggcaa gtgggtcgag
    23761 atgtgattgg ctcgatgacc aatatccttt tgttcgtcta tatcagtggg tcagttccat
    23821 cgcttctttt atatttagga aatcaatggt cgttcaaaga aacgatagaa cagcatttat
    23881 cattggaatt tttacgcgtc attgctggca gcttaggaat agtattatcg atccctatct
    23941 cggtcctctt ttttctgact gtaaggaggc tgaaacgatg aatgtactta ttatacttgt
    24001 tgggatttta gcaatcagcg tgctacttat tggcggtacg caaggtatgt atattctttt
    24061 aggcttattc atcaatttag ggatcttttt cctgttgtta ttcggttatc atcagaagtg
    24121 gccgatactg gcattgtcgg tcataggatt tttattgatt gctgtggtta tcttattttt
    24181 tatcaacggt tataatctaa aaatgcgtgc agcttttgtg agtatactga tctttttatt
    24241 ttgtttccta ctattgcttc ctatcactga ttttttagca atccaaggtt ttacatcggt
    24301 agaactagaa gaattatctg gcttagataa gactttagcg attgatttta gattacttac
    24361 gagatcgttg cttctgatta gtcttagtgg tgctgtttta gatgcgagtg tagcaatttc
    24421 tagtgggaca ttcgaagtat atcaggcaaa tccgcaatta tctttttacc aattgcgcca
    24481 ttctagtttc gctattgcta aaaaaatatt ggcgtcaaca gtaatgacac ttctttttgc
    24541 attcatggga agttctttag cgttgatcct ctggtttatt gatttagaca ttccttttca
    24601 gcaagtgatc aatgaaaaat catttgttct agaatatacg atggctattc ttaccacatt
    24661 gtccgcacta cttgttcttc ctttaacctg cgtgactaca gcatatctct ttacaaaaaa
    24721 gaaagaacaa ctcaagatgg agtaaaagtt acaacttaag tcctatgaca tttttactga
    24781 atcagtgcat aatatggatg taaggaaaag cttgcaaaca attttgctta aggaggcaat
    24841 ttccatgaaa gaaagagaac gtattttaga tttagtgaaa aaaggaatcc tttcgactga
    24901 agaaggctta gatttattag aaagcatggc aactgaaaaa gacgaaaagc aaatcaaaaa
    24961 agaagcagac aaggtaactg ctagtcataa agaaaaagat caggcaagtc aattgatcga
    25021 taaacttgaa aacggcgaag aagaaatttc tgagccagtt gatccaaagg aaagagaaag
    25081 acaagatcaa gaaaacttag aaaagatctt agatgaacta gcaactgaag cgaataaaac
    25141 ttcggcgcgt ctagatgaag taaatgctca attcgctgac aataaggcag cacgcaatga
    25201 gaaacaggaa gcattgatgc agctgaatac aaaagaagaa ttaggcgagt tgacagaaga
    25261 agaattagca cagcgtcaga cgttagaagc agaaatcaaa gaattagaag ctagtggcga
    25321 tacactgtta gaagaacaat tgaaattaga agcagaatta aaagatatcc gtaagaatca
    25381 atggtctgag aaaaaagaaa cgtttactga taagtttgaa ttgccagatg attggaaaga
    25441 tcaagcaaca gatacactga atcaagtagg cgaaaaaatg tcagaagctg gatcacagtt
    25501 aggcaaattc ttgaagaaaa cattccaaac tgtttcagaa acagtgaatg acaacatgga
    25561 atggaaagat gtcagcttac gtgtccctgg gattgcgaca acgaaatttg agcatgaatt
    25621 ttactatgaa gctcctgcag caagtatcct tgatatcaag gcagcaaacg gaaacgtgac
    25681 gttaaaaacg tgggacagca acgatgtgaa agttgaagct aagatcaaac tttacggaaa
    25741 aatgggtgct gaaccatttg aagcattctc tgaacggagc cagattgagg ttaatgaaga
    25801 tcatatctcc ttccaaattc caaataaacg tgtgcgtgca gaccttgttt tctacttacc
    25861 taaacgagta tacgaccacg cagctattaa acttctaaat ggcaacatca tgattgaaac
    25921 attggaagct aaagatattt atacaaaatc tacgaatggt aacatcatcg tggaccaatt
    25981 aactgcaacg atgctggaag tagaaggtgt aaacggcaat atcgatattc gaaacgggaa
    26041 catcttagat tctattatcg aaacagtaaa tggtacagtg acatttggtg cgacagcaga
    26101 aaacctttct gtgtctttgg taaatggtga tgtacgtctt acgattaaag aagataattt
    26161 gaaaaaagta gaagcaagtt cagtgaacgg aaatgtcaaa gtcgctttac cagatggcat
    26221 cggtttggaa ggacatgcta aaacaagtct tggaagtatc aacagccgtc tgtccaatta
    26281 cgaagtagtt cgtgagaaaa aagaacggac aaaccaaatg ttgcaattcc gtcgtgtttc
    26341 agatggtgaa atcgcgcaag tgcaactttc aacgacaaca ggaagtatct atttaaaaga
    26401 tacagataaa taaagaggtt gtgaaagaga tgttcagcct tgagcaataa gaagcgattt
    26461 tgtaaaatag cttcccctat tttacaaaat tgtgccttat tgccgatagt ctatctcttg
    26521 aacaccgttt accaagagat tatgaacctg ccaattatcg ccgagtcaca aggaataatt
    26581 tcagaaaaag ctcgtcacgt tttatgaata tggtaatttg tgaccgagaa ttttgcagaa
    26641 agacaccgat taagaagaag gttgtcacaa cgattttctt catttaaaat agtagaacag
    26701 aggtgagaca tgatgaaaaa aaagttgaca aaatctaata aaaatgttgt tttgacaggg
    26761 actttagcag gaataggaga atacatcggg attgatccga ctgttatccg tgtgctttat
    26821 gtattcctga gcttagttgc ttttggaagt ccaattattt tatacatttt attagctttg
    26881 attatcccat ctgcgcgttc tgcacgaaca aactatggac ataacaatgc atattatgaa
    26941 aaaaacaatt atcgtgaaca aactcgtgaa caaacaaaaa aacgcaaaga agcggaaaaa
    27001 atcgatgatg acgaatggag tgacttttaa tgtcgtactt tcaacgtatt ttagttaata
    27061 cgttaacatt catatcattg gctgttcttt ttcctgcgat ggtccatgtg agcagtattt
    27121 ggacggccgt tcttgccagc ttcgtattgt ctgtgttgaa tatgtttgtc aaaccgattt
    27181 tgacgatact gtccctgccc tttacattag tcacattggg attgtttacg tttgttatca
    27241 atgcagcgat tcttaaaatg acctcctttt ttgtgggagc agaacatttt agtttttctt
    27301 cattcggtgc agcgttgtta gtagctatct tgatgtcgct tgtcaatgta attgtcagcg
    27361 aacataacag tagaaaataa aagaaagctg tgccgaacta tcggcgcagt ttttttattt
    27421 agaatcagga atgcgttttc tattacattg gtaattaaat aaaaacgttt tttttaattg
    27481 gtagtacttt tggctagaca atgataaaat gatagagaac actctaatag aaatgaggta
    27541 gcatgaatgt cagaagttgt aaaagttagc caattagtga aaaaattatc tttggaaatc
    27601 gtgacaggcg acgagaaaag cttagaccgt gtcatcgtaa ctggggacat ctcacgccca
    27661 ggactagagt tgactggtta ttttaattat tattcccata atcgtttaca gctatttggc
    27721 agtaaagaaa tcagttttgc ggaacggatg atcccagaag agcgtttgat catcatgaga
    27781 agattatgta gtgaagatat gcccgcattt attatttcaa gaggattacc agctccagaa
    27841 gaaatgatcc aggcagcaaa tgaaaatggg atagctgtac tgcgttctcc catctcaact
    27901 tctcgtttat taggagaatt atccagttat ttagatggtc gccttgcccc tcgaacaagt
    27961 gtccacggtg ttttagtgga tgtttacgga ttaggtgtac tgattcaagg tgacagtggg
    28021 ataggaaaaa gtgaaacagc gttggagttg atcaaacgcg gccatcgatt gatcgcagac
    28081 gatcgagtag atgtttatca gcaagatgaa ttaacggtga tcggagaacc gcctaaaatc
    28141 ctggaacatc tgattgaaat ccgaggaatc gggatcattg atgtaatgaa tctttttggt
    28201 gcgagtgcgg tacgtggctc tatgcaagtc cagttggctg tttatctgga agcatgggca
    28261 aaagataaga aatacgaccg tctgggctct gaagacacga ccgttgaaat cgcggaagtg
    28321 ggcattccac aaataaaaat ccctgtcaaa acaggacgaa acgttgcaat catcattgaa
    28381 gtagccgcga tgaatttccg cgcacgtaca atgggctttg acgcaacgaa aacatttgaa
    28441 gaaagattaa gtcgcttgat tgaagaaaac tcaggcaacg actagcaagg agtgacaata
    28501 tgttagcaca aatcaatcga gttgcttttg agcttgtagg gattcctatc tactggtatg
    28561 cattgatcat cgtatcaggt atcattatcg ctatgtggct tagcagcaga gaggctgttc
    28621 gggtaggatt aaaagcagat gatgtgacag attttatgtt gtggggattg cctatctcca
    28681 ttattggcgc tcgcttatat tatgttttat ttgatcttcc ccagtatata gctgatccaa
    28741 tccaaatatt caatatccgt tcaggaggct tagctattta tggcggactg attgcaggcg
    28801 cgatcacgct gtattttttt actaaatatc attttatttc tttgtggact tttttagata
    28861 tcgctgcacc aagtgtatta ttagcacagg caatcggtcg ttggggaaat ttcatgaacc
    28921 acgaagcata tggaccagac acgacgcgaa gcttcttaga aagtctgcat ttaccgcaat
    28981 tcatcattga taatatgtat atcgaaggga tctatcgtca accaacattt ctttatgaat
    29041 cactttggag tttgattgga tttattttgc ttttgttttt acgaaagaaa ccacaccttt
    29101 tgaaaaaagg cgaagtcctt ttatgttatc taatgtggta tagtttcggg cgtttcttta
    29161 ttgaaggatt acgtacagac agtctttact ttttaggtgt gatccgtatt tctcaattat
    29221 tatctgctgt gttatttgtc ggtacattga ttctatttat ttggcgcaga aaaaaaggaa
    29281 atttaccgga ttatgaccgc tcattaggaa aaaatcaaca aattatctga tttttagagc
    29341 gttaaaaatg aaaaaaattt caggagtggt aaaatgaatt atagagtagc ggttttagga
    29401 ccaggctctt ggggtactgc tttggcacaa gtcctcgccg aaaacggaca tgacgttcgg
    29461 atttggggac atcgtgcttc acaagtagaa gaaatcaaca cccaacacac gaatcaacgt
    29521 tatttaccag atcattatct acccgattcc atccaagcat tcaccaatat ggaagatgca
    29581 gtaaaagacg cagatgcggt gctattcgtc attccaacga aagcaatacg ttctgttgca
    29641 aaagaactag tgcctaaatt gaatacaaag ccagtgatca tccatgcaag taaaggatta
    29701 gaacagggaa cacataaaag gatctctgac atcttgttag aggaaatccc agcagaaaaa
    29761 agaaaagctg ttgttgttct ttctggacca agccatgcag aagaagtagc aaaacatgac
    29821 atcactacga tcacagctgc aagtacagat gcaaaagcag caaaatatgt acaagagctg
    29881 ttcatgaata attattttcg gatctatacc aatcctgatg tgatcggtgt agagatgggg
    29941 gcagctttga aaaatattat tgctattggt gctggcgcta ttcacggctt agggtttggc
    30001 gataatgcaa aggcagcaat catgacgcgc ggattagctg agatcagccg tttaggtgta
    30061 gcaatgggtg ccaacccgct gacctttatc ggattaagcg gaataggcga tctagtcgtt
    30121 acttgcacaa gtgttcattc taggaactgg cgcgcaggca acttgttagg acaagggcag
    30181 cccctagaag aggtgctagc caatatggga atggtcgttg aaggtgtgaa tactacccaa
    30241 gcagcgatgg aactagccca aatgatggaa atcgaaatgc ccatcactca aacaatctat
    30301 gaagtgctgt atgaacaaaa agaaatcaaa acagccgcaa aagaaatcat gttacgtgat
    30361 ggaaaaaaag aaaatgaatt ctcattaaca attttttgaa ggagcgaatg tatatgaaag
    30421 ttaaaaaagc agttattcca gcagcaggat taggaacaag atttttacca gcaacaaaag
    30481 ctatggcaaa ggaaatgttg ccaattgttg ataaaccaac aatccagttc atcgttgaag
    30541 aagcgttgaa atcaggtatc gaagacatct tgatcgttac aggaaaagct aaacgaccaa
    30601 tcgaagatca ctttgattca aatattgaat tagaaacaaa cctaagtgaa aaaggaaaga
    30661 ccgaattatt gaaattagta gaagaaacga cagatgtcaa cttgcatttc atccgtcaat
    30721 ctcatccaaa gggtttagga catgccgttc ttcaagcaag agcctttatt ggaaatgaac
    30781 catttgtcgt tatgctaggc gatgatataa tggaagacga agtaccattg acacagcaat
    30841 taatcgatga ttacgaacgt actcatgctt ctacgatcgc cgttatgcaa gttcctcatg
    30901 atgaaacttc aaaatacgga attatcaatc caggagaagt attggaagat gggttataca
    30961 atgtcaaaaa ctttgttgaa aaaccagatc caagcaaagc accaagtgat ttagctatta
    31021 ttggacgtta cttattgact ccagaaattt ttgatgtatt gtccgagcaa caaccaggag
    31081 ccggaaatga aatccaacta actgacgcaa tcgatacatt gaataaaaca caacgcgtat
    31141 ttgcccgtaa attcacaggt aaacgttacg atgtcggtga caaatttggt tttatgaaaa
    31201 caagtattga atatggtcta acacatccag aagtaggtgc acctttacgt gagtacatcc
    31261 tgtctttagg taaaacatta gaagcagaag gaagcaagat cgatcataat ccaaaaggaa
    31321 taacagaaat caaagaagaa aagaaaaaat aacccttttt tctaacgagc ttcagctgct
    31381 aattgaataa aaagtagttg aagctcgttt ttatttgttt aaaaaacacg aaaatgctat
    31441 ccttatagaa aagagagaca agaagggagt ttgatcatgt caggaggaga aattgcagga
    31501 ttgatcgcag caatcgcctt tgcgatactt gtcgttttta tcgttcgtgt gttgttgcaa
    31561 gtttctaaaa ctttagaaaa agtagatcag actgtagcag aagcaaatac gacgatcgaa
    31621 atcgtcacaa aagacgtaga tcttttgtct agacaagtag aaggcttact ggtcaaaagt
    31681 aatgaactgc tcttggatat caatggaaaa gtcgcaacga tcgatccatt gttcacagca
    31741 gttgctgatt taagtgaaag tgtatcagaa cttaatcatt ctagtaaaaa catcgcgacg
    31801 aaagtcggcg gaatcggtaa aacgacagct aaagcgacgg ttgccggaaa agtcggtggg
    31861 acagcgatga aattttttaa aaacaaaaaa catactgaaa cgacaggagg aaaataaaaa
    31921 tggctaaaaa aggtggattt ttattaggtg cagtagtcgg aggcacagcc gcagcaatcg
    31981 ccgcgttatt gttagcacca aaatctggta aagaacttcg ggatgaatta gctgcccaag
    32041 cggatgattt gatggatatg gcttcagatt acacagatat ggcgaaagaa aaatcgactg
    32101 agttaacagg tattgcaaaa gaaaaagcgc aagatctttc tcaacaagca ggtgatctag
    32161 caggtagtgt caaacaaaaa gtaagtgaat cagcagatga agcaggaagt gaatctgacg
    32221 atatcttgtt aaatcttaaa gaacaatcag ctgacttatc taatcgtttc aaaaaaacgg
    32281 cagaagatat gaaaaaacct gcagatgaat taggaagtat tgctaaagat acttcagaag
    32341 atatcatgat tgatgtaaaa gacgctgctg gtaaagcagg agatactgtc aatgaaggaa
    32401 tgtcagaagc aaaaccagtt gcagatgact caaaagaaat gatgaaaatg gcaaaagaag
    32461 atgcgaaaga agctaaaaaa gaaataaaag atacaatgac agattcatct acaaatcact
    32521 aaaatcacaa atttatagaa taaaaaggtg ggagcggact agctctcacc ttttttactc
    32581 ttatttttct aaaacaagta attcttttgg tgtagtcgtg aatggcacac agccatcgct
    32641 tgtcacatat acacagtctt cgatacgtac ccctactttt tcaggaatat agattcctgg
    32701 ttcaagagag aagcacatcc cttcttcgat tactaagtca tttccttcta caagggaagg
    32761 gaattcgtgg acggttgttc cgatcccgtg acctaaacgg tgattgaaat attcgccgta
    32821 gccatagctg ttgatcacgt ttcgtgcgat ttgatcaagt tcacctgctg tcacacctgg
    32881 acggacggct tcttgtgcgg caagctgtgc ttcaagagtg atgttgtaga ttttttcttg
    32941 gaagtcggtt ggtttttgat aagcaattgt acgagtagca tcactgcaat agccattcca
    33001 aacaacgcca agatcgaata acacaagttc attcggtgaa atcttcgtat ttcctggtgt
    33061 accatgaggg cttgcagcat ttgctcctgc aaggactaat gtgtcgaaag acatttgtga
    33121 aacaccttgt tttttcaatt ggtattcaat ttctgcaatg atcgcttgtt cagtcacgcc
    33181 ttctttcact gctttaaagc caatctcaaa tgcaacatcg gcccatttac cagcttcgat
    33241 cagtttgtct ttttcctcta gagttttgat cagttgtaga cgttggatga tggaagttag
    33301 atcagtgtaa gaatccgttc ccgtcaagat atcagagata gcatcgaagc gctcgacagt
    33361 caaggcgttc ttttcaatcc ctacattgcc aggctgacca tagcgagcac gaagttcttt
    33421 tccgatgatc tcccaagggt tttcgctgtc taaatagcca cggacatcat acgcccaact
    33481 actgttttga gcatcttcaa cttccaaagc aggagtgaaa aggaaaggat cttgttcaag
    33541 agggataaaa agagctagta cacgctcatg aggatcactt tcgtatccgg aaaagtaagc
    33601 gatgtggcca gggtcgctga tataagccaa ttcgacttgg tgttgtgcca tccattgacg
    33661 tagttcattg atttttacgt tattcatttg gcaacctact ttctaaatta attttgtatt
    33721 caataattgt accactgatt tattaaaaga ccaacgttct aaaaactgaa aaaagtaaaa
    33781 attttcatga aaacagagat aaaacggttg caattcttga gattatttga tattctattt
    33841 tagaaatgta agacgtattt tcagaaaaca tataaaatat gaataggaga acacaaatgg
    33901 aaaagcaaac aatcactatc tatgatgtag cgagagaggc aaatgtttcg atggctactg
    33961 tttcacgtgt cgtaaatggc aatccaaacg ttaaaccagc aacaagaaaa aaagtcttgg
    34021 aagttatcga tcgcttggat tatcgtccaa acgcagttgc gcgtggcttg gcaagtaaga
    34081 aaacaacaac agttggtgta atcatccctg atgtcagcaa tatgttcttt gcttctttag
    34141 cacgtggtat cgatgatgtg gcaacaatgt acaaatacaa tattatctta gcaaattcag
    34201 atggcaacga ccaaaaagaa gtcaatgtat tgaataactt actggctaag caagtggacg
    34261 gcgtgatctt catgggtcac catatcacag acgaaatccg tggagaattt tcacgttcaa
    34321 aaactcctgt tgtattagct ggctctattg atccagatga acaagtaggt agcgtaaaca
    34381 ttgattatgt atccgcaaca aaagatgcag taaacaaatt ggcaaaaagc ggcaataaaa
    34441 aaatcgcttt tgtcagtggt gcgttgatcg atcccatcaa tggacaaaat cgtctgaaag
    34501 gctataaaga agcattaagc gaaaataacc taccatatag cgaaggattg atctttgaag
    34561 cacaatacaa cttcaaagat ggtctatctc tagtcgatcg gatccataat agcggtgcaa
    34621 cagctgtcta tgtttcagat gatgaattag ccatcggtat tttggatggt ttattagacc
    34681 gtggcgtgaa agtaccggaa gaattcgaaa tcatcacaag taacaattca ctactgacag
    34741 aagtagctcg tcctcgttta acaagtatca cacaaccatt atacgattta ggggcggtat
    34801 ctatgcgcct attgacgaaa atgatgaata aagaagaagt cgaagaaaaa acgattatct
    34861 tgccttatgg aatcgaagaa aaaggatcta caaaataata aaaggacaac cattcagagg
    34921 ttgtgtgcct gccgtttaac tctgcgttac aaggagcaat ttcaaaaaac agcatctcat
    34981 gtttgatgaa tattgtgact cgtgattcgg agttggcagg agaacaccgt ttatttggga
    35041 aaaggggtta tgacaaaatt ttgttataat cctttttttg tgtcttaaaa aagagagaaa
    35101 gtggttgaaa ttacatttat cgtatataaa acaatccgtg tatagatgct gcattttgtt
    35161 caccaccatc aaaaaaataa gggactgtga caatactatt gtcacaatcc cttattactg
    35221 tgtattctat taaatcaatc atctttctca tctgattctt tagttgcttg attcgctctt
    35281 gcataagctg aagcggtgtt ccagtaatct gtatagtttt catcggtacc gccaacgcca
    35341 aatttaaagg cgcttttctt cggtccattt ttcgcccata gcgattcaac tgtttttcct
    35401 ggtgtttgga tggatttttt attgacagta acttttccag gtgtttgacc agtaaatcca
    35461 gatacttttt tcttcatcac atcagatgat aattcgaatt tctcatcagt gccaaaaatg
    35521 tcaggttttg cttggtaagc ttgattgatc agattggcaa gataattagc cgaacgtatc
    35581 cttgctttgc tgtccattcc agtattgtca tcgtgccctg cccagctact gatggtaata
    35641 gacggagtag agacaattag ccatgaatct cggtattcat tggtagaacc agtttttccg
    35701 acccaatctg cttttcctag atttccgttt aagcttgaga tggcatcttt gaatggtgta
    35761 gtgatttttg cattgatcac actacgcatc atgtcattca tgatcgatgc cgttgcttcc
    35821 gaatagatcc ggacagagtt gctttcgtgt ttataaatta catttcctgc gttatctgtg
    35881 atggaatcga tgatatagcc ttcttcatag acgccgccgt ttgctaaagc ctgaaatccg
    35941 ttggtctgtt gaagcgtcgt gacatctact gtacctaaag gagcagattc aacgccccaa
    36001 ttgtcatttg ccgggtagtt catctttgcc aagtagtgct cgtaagcata ttgtttcgat
    36061 ccaccggaat tcaacacatc ttgatataag tgataagccg ggatattgtt ggaccattct
    36121 agtgactcac ggacggtttg gaaggtgttc gatccttcgt tagtagcatt gacgattttc
    36181 tcgccagcat tttctccttc ttgccaagtt gttgcataat ctgatacacg ggattctgat
    36241 ccgataagcc ccatgtcgat tgcaggtcca taaacaagaa ctggtttgat tgatgagcca
    36301 gcttgacgtt ctgtatcgaa ggcatggtta ttttgattct gactgtaatt ccgtccgcca
    36361 acgaatccgt agatgcggcc agttctgtta tccatcaata catttccggt ttctacttga
    36421 ctgccagcac catcatctaa catgtatcca taattttcaa cgccattttg caatgcagca
    36481 tagacatctt tatcgattgt cgaatggatt gtatatcctt tattttggat cgtttgctgt
    36541 gccttttgat aataagcgtc actgaccgat gaatcagaca tatcagcgtt gtctttttca
    36601 gccaattgtt gtgcaatgat tcttgttgct tcattcatca cagtataata caagaattct
    36661 ctgtcagtct gttctgcgat ttgttgtggt agaaagtctt ttgtaaggtc ataggctttt
    36721 gcttcttcgt attgttcttt cgtaatttgg ccttcacgat acatactgaa taaaacaaaa
    36781 tcttttctgg caaggccggc agaaagatct tcttttagcg cgcctgtgtt cgtgtatgga
    36841 ctatacgtga tcgggctttg gggtaatcca gcgatataag cagcttgcgg cagcgtcaca
    36901 tcttttgcat ctacaccaaa gattcctttt gcagcttcct ggactccagc aatgttttgt
    36961 cccttattat tacgaccaaa tggtgagaca ttcaaatacg tagcaatgat ctcatctttt
    37021 gaaaagtatt tttctacttg tgcagaaaga atgatctcgt ttgctttacg tttgaatgtt
    37081 gtttcatctg taaggatctg ctgtttgact aattgctgtg tcaaagttga tccaccagaa
    37141 gagccaatac cagtagcttc agatactaat gctcgtaaaa cagctttagg aacgtaacct
    37201 tggtgtttat cgaagtattc atcctctgtg ctgataattg cagttttcaa taaaggtgaa
    37261 atcttatcag atgaaatagt cgtgcgcatc agatctgtct ggatttttga tatctctgta
    37321 ttatctgcat aaacaatctt tgacgtttct gtaatgtttc ctagttctgt ctgcagtgct
    37381 tttttagtcg gcaattgtgt gtcttcgacg agataggcaa aatagccagc gcctattcca
    37441 atacctagag ctgcgaacat caaaagaaga acagtaataa aaacagtcaa tgattgaaaa
    37501 actctaaaaa taatcttagg aacaaaccat ttttcttttt ttcttctatt ctgttttttt
    37561 cgagtatttt tgcgtggcaa aagggtcacc tcaactaatg gttttatttc gctaattata
    37621 gcaaataata gaacaaaaaa acagacaagg ctgtttttgg caggaatttc ttttttaaaa
    37681 gagttaaaac gtgagaaaat gatctttatt ataaaagagt tcttaagctt ttctttagag
    37741 aaaatttcag ttactatgga gaacagggca aagaaaaatc aatcattttc tttgtatatc
    37801 atcttaccaa tatataggaa ttgttagtaa caattgcctt tcaaggagga atccatccat
    37861 gttattaggc tcacacgtca gcatgagcgg aaagaaaatg ctgttaggag cagcagaaga
    37921 agcagccagt tatggatctt cgacttttat gatctataca ggcgcccctc aaaacactag
    37981 aagaaaagcc atcgaagaga tgaatattga tgccggaaaa gcgtacatgg cagaaaaagg
    38041 tctaacaaat atcgtcgttc atgcccctta tatcattaat ttggggaata cgatcaaacc
    38101 agaaatgttt ccttttgccg tccaatttct gagagaagag attgttcgtg cggaagcttt
    38161 aggcgctact caaattacac ttcacccagg tgctcatgta ggggcaggaa cagaagccgg
    38221 aattgctaga attattgaag gcttgaatga agttttgaca aaagaacaga cggcacaaat
    38281 cgctttggag acaatggcag gtaaaggtac ggaaatcggt cgaacatttg aggaattagc
    38341 gcaaatcatc gatggagtaa aattgaatga aaaattatcc gttacttttg acacctgtca
    38401 tacgaatgat gccggttata atattaaaga agacttttca ggtgtaatgg aagagtttga
    38461 taagatcatc gggttagatc ggcttaaagt actccatatc aatgattcta aaaatccgca
    38521 aggttcgcat aaagaccggc acgccaatat tggttttgga acaatcggat tcgacacgct
    38581 gaataagatc gtccatgatc caatgtttga caatatttcg aaaatcttgg aaactcccta
    38641 tgtaggcaaa gataaaaaga aagcttacgc accatatgga aaagaaatcg ccatgttcaa
    38701 agcgcaaaca ttcgatccag aagtgtttcc tgagttatta gcagaagaag tgatactcta
    38761 agattttcta tctgacagca gcaaaaaagg accaggcaca ttgccttgtc cttttttgtt
    38821 taagaaacaa aggagtactt tttgcagtct ttttcatcat gcatcttctc ccccctcttt
    38881 ttaaatttag acaaacaagg tatactggta gaaaagtaaa aaaacgaaaa gtttcacaag
    38941 caagtggcaa aacgtgattg gaaggacgaa aagatgctga acactttaaa acccttgatc
    39001 aaccaactaa aaaagaaaca tacatacttt attcttggca tagccctatt atttacaatt
    39061 attcgtttgt atttatatag taaagcaatc tatggaacag acatgtcggc aacctatgat
    39121 gaccagctgc tgattcgcta tgctcaaaat attttggacg gtaaatggct aggggagtat
    39181 acgactacaa cactttccaa aggaatctcc tacagcttat ttttagtact tgcacataaa
    39241 ctgcatcttt cttatgcagt tttgttcggc atgttcagta tttttgccag tatattggtt
    39301 gcactcgcgt ttaaaccgat tgtgaagaac aagtatcttc ttttaagtat ctatttttat
    39361 tttttgtttt ctccgattag ttttacacat gaatattcca ctcgaacata tcgtaatgcc
    39421 ctcgtgattc cggctgttga gatcattatc gcttgtttat tagctattta ttaccgcaaa
    39481 tacggttcta gcagaaaatt gctgccatgg tttttagggc tttctgtcgc ttttcctttc
    39541 ttttggttca ttcgcgaaga ttctatctgg cttcttcctt ttactgtagt agctttgggt
    39601 atcagtttcg tccagatctt gctagggcaa gacttttctt tcaaagaagt aaagaagtta
    39661 aaaaaacgac tctttcttct ctcgaaacgg gaatggctga aactttcatt gtttttactt
    39721 cctatagttg gcttttccat cgtctatcaa tggatcgaat ataaaaatga gacaacatac
    39781 ggtattgcaa ccatcaatga ccgttccagt ggagaatttg gacgtttgat gaaacaactg
    39841 atccgaatag atgatgaaac gaacttgaat aaagaaaaca gtgagatatg ggtctcacat
    39901 gctgcactaa ataaagcatt agccgcatct cctacattcc atactgtttc tacccgtatt
    39961 gacgaattat atacgactca cgcgtggact caaggtggaa aagtaaaaga attacccgga
    40021 gatatcatct tttgggcatt gcgagatgtg gtaagcgaat ctggttatta ccaaaacaat
    40081 gcaaaagaaa ccaatgattt ttggaaaaaa gtcaaccaag aattagaaga agcctatcac
    40141 aagggaacaa tcaaaaagaa aaaagagttc tatctgatgg gatcaggaga tggaaagacg
    40201 atgagtgatc tgccaatcgt attcgatttc cttcaatcag gatacgctta tgcatggcga
    40261 tataaagatt tccatcaagg aggaaattat agtttcggaa cgcaagaaca agtcaaacag
    40321 gctcaagaat tattaaacct tccgctaatg gagaattgga tcgatagtaa tgaaccgaga
    40381 acacctaatt tccaattaac aaaatcagca aaagtcgcga atgtcatcat aaaaatttac
    40441 caagttctgg ctattcccgt gctgattgct tcaatagcag ggatattact tatgatacta
    40501 ggaaccgtat ttgcaaaagc tgatcgtgcc tattatagtt cctctttgat tgtggtcacc
    40561 ggattaatac tgacacaatt ggtttttctt ttcggtgtct catggttttg ttcttatgcg
    40621 ccagaaaaaa aggactattt tttaagtgtc tacactggtg caggcgtgcc attaacgcaa
    40681 ttcatcgtag taacaagcat gctagttttc agtaaattaa atctgaaaaa agctttatct
    40741 tctaaataaa aagacccttg gtagctagat ttttaaccta actatcaagg gtctttgtgt
    40801 ggaaaaatca gtttttattc gatttcttct accgtttcct gcaaaagctg ttcatatttt
    40861 ttgtttcctt tatataaatc accaatcgtc catgccaaca ggagaaggac aagaacaatg
    40921 attacataag cgatgtgatc agccaatgct agttgagcgg aagtggcttg ctcgccgaag
    40981 aaactttcga tctgtcctgg taatcctcgt aagttcaagt aagttagact aatcacagag
    41041 aaccagccaa gaagttttac gatgaatgaa tttttaaatc ggctacccat ctccgcttgg
    41101 ctgttggtca tcatcagtaa aggaatcatc gaaaacggca acgcaaaagc caagaacacc
    41161 tgggattcat tcattaagtt attcaaagcg gcatgttctt cgatcgttcc ttttgtactt
    41221 gtaaacaaaa cacaaatcaa tacaggaatc acagaaagca atcgtgtgac cagtcttctt
    41281 agccaaatcg ggatacgcat atggatgaat ccttccatga tgacttgacc ggtcaacgta
    41341 ccagtaatag tagagttctg gccagaagca agaagtgcta cagcaaataa agtggacaag
    41401 ataccagatt ttgctacagc aatcaaaata ccattgctca tagctgatgg atcagataaa
    41461 gcttcataca gaccaaaaaa ggaaggatct tggacagaac cggttttgaa gacagcgaca
    41521 cccatgatca atagcagtgc attgactaga aaagccattg acaactggat attagaatcc
    41581 caagtagaaa aacgtacagc ttgtgctaca tcttcttcgt tttggtgatc gatcttccga
    41641 gtttgtgaaa ctgctgaatg aagatataag ttatgcggca tgactgttgc accaataatc
    41701 cctaaagctc cagtcagcgg agtttcgccg ccgatgaccg gtgagtctga gaatgtatga
    41761 ctgttaggga taaagccagc aaataaactg tgccaatctg ggttagacaa gccaacttga
    41821 tagacaaaaa cgaataaaat gacgaaaatt aagcagacaa ccagtgcttc gatcttacga
    41881 aacccgactt ttgtcagtag taagagaacg aaaacatcta atacagtaat aaagacggca
    41941 atcaccaatg gaatatcaaa aagtaagtag agggcgattg ctgcaccaat gacttctgca
    42001 atatctgttg ccataatggc taattcagtc aagatccata aaatgacacc taatgtctta
    42061 cttgtgcgtg ctcggattgc ctgtgccaaa tccatttggc tcacgattcc taattttgct
    42121 gccatatact gtaaaagcat agcaatcaga ctagacatca aaataactga catcaataaa
    42181 tattggaaat tttgtcctcc ggtgatcgaa gtagaccagt tacccgggtc catataccca
    42241 actgctacta acgcaccagg tccagagtaa gcaagaagtg ttttaaaaaa acttttcccc
    42301 tttggtacat caactgttcc atttacttca gctaaagata aaccatttgt atgttcaata
    42361 agtcgatgct tatgaaattg tttattggat tgattattca agaaaaaacc acctttccta
    42421 tttatctacg attacattct acacctcaaa aagaaaaaat taaagaaaaa gattcggtac
    42481 gcctaatttt ttttcatggc gcgtcttgct agatgacgaa agaaattttg ttctcctcta
    42541 aaaaaaccac tattttttgc atgaaactaa agaatacgcc tttctttttt caataaactt
    42601 tagtaaaata agtcattgag agcaattcgg aaatagaaag gagagattta tatgttatca
    42661 acaggtattg tcgtacttat tgctattatt gctttgctaa tcggcgcagt aggtggcttt
    42721 ttcctagcgc gtaaatatat gaaagactat ttagaaaaaa atcctcccgt taatgaggac
    42781 atgttgcgat caatgatgat gtcaatgggg caaaaaccat ctgagaagaa aattcgccag
    42841 atgatgcagc aaatgaagaa ccaagggaaa aaataggttc tgacccaacg actttttacg
    42901 ggctgatttt ttcagtccgt tttttatttc gtaatttgtt agactattca gattattcag
    42961 caaaaaagac aagcacagac aatcctaggc tcttgataaa gacaaaggag gagtaaaatg
    43021 tcgattttta gaaaattagg ctggtttttt cgtcaggaaa agaaacatta tttaattggg
    43081 gttttctcgc tattcgcagt ggccttggta cagttagttc ctccaaaagt gattgggatc
    43141 atcattgatg agatcgccga taaaaattta tcaataaaga tcattttcgg gtggatcttc
    43201 ctattgatta tcgctgcttt tttacaatat ctttttcgtt atatctggcg gacgaatatt
    43261 tggggaagtg cagctcgatt ggagaaagaa cttcggcagc agttattcaa acattttaca
    43321 gaaatggaca atctctttta ccaaaaatat cggactgggg atttgatggc tcacgcaacg
    43381 aatgatttga atgcgatcca gaatgtagca ggagctggta tcttgacctt tgcagattca
    43441 gtgattacgg gcggaacaac gatcattgcg atgattttgt ttattgattg gcgcttaaca
    43501 ctgattgcgc tgattcctct gcctttgctg gccgtcactt cacgagtgct ggggtcaaaa
    43561 ctccatgatg cttttcgtga ttcgcaagaa gctttttcta gtatcaatga taagacacaa
    43621 gaaagtatca ctgggatcaa agtcattaaa acatttggac aagaaaaaga agatctagcg
    43681 gattttgaag aaaagattga agatgcaatt attaaaaaca aacgagtcaa ttttttagac
    43741 tccttatttg atccattcat taccttgatt atcggtctgt cttatgttgc aacaattatt
    43801 ttaggtggaa agtatgtctt ggaaggcacg atctcgatcg ggcagatggt ctcatttatt
    43861 agttatatcg gtatgcttgt ttggccgatg tttgcgattg gtcgattgtt caacgtattg
    43921 gaaagaggaa atgcttctta tgatcgcgtc aatgaacttt tgcatgaaaa gacgcatatc
    43981 gtggaacaac tgggtgcagt gcagaccccg gcaaaaggcg agctgaatgt agctatttcc
    44041 tcttttaatt atccgaaaga agaatcacca gcattagaac aaatcaagtt tgcgatccaa
    44101 gaaggagaaa cattaggtat tgttgggaaa actggtgcgg gaaaaacgac gatcatcaaa
    44161 ttactgatgc gtgaatacga tgattatcaa gggaaaatcc aatttggcgg acgttcgatc
    44221 aaagaatatt cattgaatgc tttattagga tcaatcggct atgtgcctca ggatcacttc
    44281 ttgttttcga tgaccattcg agataatatc cgtttttcta atccaaaact ttcagaagaa
    44341 gaagtagagc atgcggcttc tttagcattt atcaacaatg aaatcaagga attaccaaaa
    44401 ggttatgata ccttagttgg agaacgtgga gtctctttat ctggcggaca gaaacaaagg
    44461 atttcgattg ctcgagcatt gatcaaaaat cctgaattat taatattaga tgatgcactc
    44521 tctgcagtag atgccaaaac agaagaagct atcttgcata atttaaaaga aataagaaaa
    44581 gagaaaacaa ctatcattgc agctcaccgt ttaagtagcg tgatgcatgc aaaagaaatc
    44641 attgttgtcg atcaagggaa aatcattgaa cgaggaaccc atcatgagct gcttgcacta
    44701 ggcggctggt atgaacaaat gtgggagaaa cagcaattag aagcgaagat agaaggaggc
    44761 gaggcctgat ggatgaaaat tatcaatcgg aatggacaaa atcgattcca cttaaagaac
    44821 agaaaaagat catcaaacga ctgatgaagt tcgccaaacc ttttagaaga acgttcatcg
    44881 ttgctatctt atttgcgttt gctttgtctg tgatcaatgt cctattgcca aggatcatcc
    44941 aaacttttat ggatgatcat ctggctaaac agtcggctac gacacaagtt attttgtttt
    45001 ttgctggtat gtacttattt ggagtcatag taaaaagtat catttggttt ttccaatggt
    45061 atttgtattc catggcctca ttgaagacct atcagtatat tagggtaaaa ttatttgaaa
    45121 agctgcatac actaggtatg cgttattttg atcagacacc ggcaggatca accgtttctc
    45181 gagtgacgaa tgacacagaa acgctatttg aattttggta cgtctttttg atggtattga
    45241 ctgggatctt tgcagtcgtt tcttcattcc ttgccatgtt tcaaatcaat ggagaaattg
    45301 ccttgtggac tttggtgttt ttgccgattt taggaatcgt catctggtat taccagaaat
    45361 tcagttctcg tatttaccga aatatgcggg aaaaattaag tcagctgaac acgaaattga
    45421 atgaatcgat ttccggcatg cgtattatcc agcagtttcg ccaagaagct cgtttagcta
    45481 aagaatttga acaagtgaac aatgagtatt tagacacgcg ttatgcgatg atcaaaacga
    45541 attcgattct gttaagtccg atcatcaatt ttctatatgc attggcgatt gcgttggtgt
    45601 tgactttgtt tgggatcgat gcgttgcatt ctcccgtaga agttggactg atctatgcat
    45661 ttacgaccta tgtccaggga ttttttaatc caatgtctca aatgatggac tttttgagca
    45721 tcttcacaga tgggatggtt gctggtagtc gtatcttgaa gattatggat acggaagaat
    45781 atgcaccaca acaaaaccct ggagcagatc aaatgattac ttcaggtaag attgagtttc
    45841 gcaatgtaag cttttcttat gatggagagc acaatgtatt aaacaatatc tcttttgttg
    45901 ctaatccggg agagaccgtt gcattagtgg gacacaccgg aagcggaaaa agctcgatca
    45961 tcaacgtatt gatgcggttt tacgaatttt atgaaggtca gatattgatt gatgatcatg
    46021 acattcgaga atatccaatg gaagagttac gtaagaaaat ggggctggtc ttgcaagatg
    46081 ctttcttgtt ttatggggat atcgcaggaa atattcggat gatgaatcca gctatcacag
    46141 acgaacagat acgccatgcg gctgagtttg ttcaagcaaa caaattcatc gatgaattac
    46201 caaaaggata tcactcaaaa gtgatcgaac atggtgccag ctattctagt ggacaacgac
    46261 agctgatcac ttttgcgcga acaatcgtga ctgatcctaa aattctagta ttggatgaag
    46321 caacagcaaa tatcgatact gaaacagaag gattgatcca agaaggattg gcaaaaatgc
    46381 gtcaaggacg gacaacgatt gcgattgccc atcggctgtc aacgattcga gatgcagaat
    46441 tgattttagt tttagacaaa ggtcatatag tagaacgcgg gaatcatgaa acacttctag
    46501 cgcaaaatgg tttgtatgca gatatgtaca aactgcaaag cagtggtata taaaagatgt
    46561 tgtgacagta gaattttgac ctaagcacta gagtgaaaat aaaacatagc ttctcatatt
    46621 tttcatctgt gagagttagt gtcgtagtca ccagatttaa aaaaagattc gacagctagt
    46681 cagaagtgcc taatagccac taagaaagcg ttgacaaaag catttgcttt cgataatata
    46741 aagaaaacga tacaacttgg atagtgattg cctgatgcaa gctaaacctg atgagtctcc
    46801 catggtttca tggacggctt tttgggtttt tctttttgtt ttggatacag ccctatacat
    46861 catggaaagg agcaaaacat gctgattgag aaagtactga ataacaacgt cgtaataacc
    46921 agggatgaaa gaaatcagga aatgatcgtg atgggaaaag gtttggcatt caaaaagaaa
    46981 gtgggtgacc cgatttttga ctcatgcatc gacaaaaaat ttcagttgac aaatgacggg
    47041 ctttcacaac agttccaaga attggcacaa gcgattcctc ttgagtactt agaaatttct
    47101 tgtgaaatca ttgaatatac aaaagaggct ctacaagtcc agttgaatga ttctgtgtat
    47161 attgcattga cggatcattt gtatacggct atcgaacgtc acaaaatggg aatatttgta
    47221 ccaaatgtat tgctgtatga tatcaaacaa ctatttccta aagagtatgc catcgggaaa
    47281 aaaacagtga agaaaatctc ggataaatac cacatcaagt tatcagaaga tgaagccgga
    47341 tttatcgcat tgcatttagt caatgcacaa ggcattcaaa gccagcaaga caatacctac
    47401 ctgtttacta taacaataca ggatattcta aatatcgtcc gctattacta taagattaca
    47461 tttgatgaag attcagtcca tttttttcga ttcgtcacac acttacgttt ttttctgaca
    47521 cgcgtcctag atggagaaaa atcagaagtg ggagaagttt ctgaggaatt gtttcaggtt
    47581 attcaaaaaa aatataagga tacgtttggt tgcgttgaga aaatagcaga ctttttagcc
    47641 gagacgttca actaccagat gtccacggac gaaaaaatgt atttaacgat ccatactgcg
    47701 cgtttagtgc atgagaatcg ttgtaaataa aaaaaggagg ataaattaac aagaacgtat
    47761 gaaataaggg tagtgacatt cagttggcta aaccttccac taataaattg gagggaatag
    47821 aaatgggaaa ataccaagaa ttagcgagaa aaatcgtaga aaatgtcggc gggaaggaaa
    47881 atatcaatgg tttgacacat tgtatcacaa ggcttcgttt caaattaaaa aacgaagaaa
    47941 aagcaaatga tgagatcttg aaaaatatgg acggtattgt aacggtaatg cgagcgggag
    48001 ggcagtatca agttgtcatt gggaatcatg tgccagtcgt gtttgaagag gttataaaag
    48061 caggaaacct tacttttgat gaggctgttt caacagaaaa aatgagaccg tttgatatgc
    48121 ttattgatgg aatatcgggc tgtttccagc cattccttgc tatcctagct gctggcggga
    48181 tgattcgtgg actgacagca ttccttgtct tcttaggcgc gtttgaccgc ggttctggca
    48241 catttgttat gtttgataat atcggtgatt ctgtattcca atttatgcct gttattatcg
    48301 gtttaacagc tgcccgtaaa tttaaagtaa atgaattcgt agggatgttg cttggcgcag
    48361 cattaatgaa tcctagcttg tcgctggaag ctttgagtgg tgcagcagaa gcaccactaa
    48421 cgaccatatt ttctggcact atttttgaag cacctattta tcaaacagta ttcggcattc
    48481 cctggattgc gcgtaactac gcttcttctg tcattcccat tatctttatt gtgttgttag
    48541 ctagccaaat tcagaaacca attaaaaagt tagtacctga gatgatcgcg aatttcttcg
    48601 ttccattctt caccgttctt attacaatgc ctttaggttt tttactcgtt ggaccagtat
    48661 tcacattcgc aactgatatt ttaatggctg gatttgaaac acttttagca ctttcgccag
    48721 ttatttatgg agcaatcgta ggcttcttct ggcaaattct ggtgatgttt ggtctccatt
    48781 gggcaatcgt accaatggga ttgatgcaat tttctgtgaa tggttggcaa aatattatga
    48841 cacctgttgc agtagtgtct tttggacaaa ctgcagcact taccgcactc tactttaagc
    48901 ttagaaatcc aaaagacaaa gcaattgcaa taccagccat tgtttctggt attgtgggga
    48961 ttacagagcc agctatttat gggtttacat taccacgtaa aaaaatattt atttttacct
    49021 gtgtcggtgg tgcgttaggg ggcgcgtata gtgggttaat gaatttaact tcttggaatc
    49081 aaggtggact aggcatcttt acaattccca actatattag accagatggt gatttaacag
    49141 atgtgattaa tgtattgatt gggatcgcca ttgcgatggt ggtatccttt acgctgactt
    49201 ttttcttttg gaaggatgaa gcaggtgaaa cagatgatat acagaaaaaa tcaggtaaag
    49261 aaattgtaaa aacaccgata caaggacaaa tcgctccact aaatgcagca aaggatgcag
    49321 cttttgcaca aggaacacta ggtcgaggaa tcttgatcta ccctgaaaaa ggagaagtac
    49381 gtgccccttt tgacggaaca gtcatgaccc tgttcccgac gaaacaggca attggaatgg
    49441 tttctgaaac agggctggaa ttgctgattc atgtcggatt ggatactgtg cagttggaag
    49501 ggaaatattt tgaaagctta gtgcagcaag gataaagagt gaaacaaggc gatctgttgc
    49561 tacgcttcga caaaaagaaa atcgaaaagg aaggatacag tttagaaaca cctgtgatcg
    49621 tcaccaatta cacggattat ttggatattt tggaaaacca aggggagacg gtcgggccag
    49681 acgatgcact tatcaccgca ctgacctgaa cggttaggtg aagaatggac taggcttaac
    49741 tattcttttt attccacttc tttattttca aaattgaaag gctctttcat gatggctgag
    49801 ctgatagggc gtttatcgaa tttttgaccg acagaaatta gatggactaa attctgttga
    49861 taattttctg caaaaacaca actatataac acgtctagaa gataacaaat tgatgtattg
    49921 atcgtaaaat taccaatttt tgaatacagt cgctcgcgtg tcgtaagtcg caaagcggct
    49981 gtactcattg cagatagcgt tgaatcaccg atactggtca aagctaaaat tggaacatga
    50041 ttttgtttca atatttctgc ggtacgaagg atcacattgt tttcacccgt ataagaaatc
    50101 agaatcccgc acatatcact cgtgcaattg tacgcttcat agaatgattc accaaaagtg
    50161 gtgacaatcg tgacatccat tttgatgcgc ttcattttca gcatgaaatc ttgagaaatc
    50221 aaagtattcg agttactggc gaaaatcacg attttttttg cttgaagcat tagttgtttg
    50281 gctttttgca gttggtcatg atgaatgaga gaaagtgtat catgaatcgt catctgttct
    50341 aaagaagcca ttttatttgc gatcgtcata ataccgtctg tggtggaaaa aggaagattc
    50401 gcatctacgt cttgaaaatt cccttcaaga taggctgttt cagctaaaaa agcattacga
    50461 aaactgaccc agccatcaaa acccattttt tttgctacac ggatgagtgt agaggggtgg
    50521 acataacatc gctcagcaat cgcttggatg gtcatttcgc caagtgctgg agaatcgctt
    50581 aagagaaact ggatcacttc attttctgaa ggagaaaagg agatttcttt gattttttca
    50641 cgtaataaca tgtagatacc ttcttcttat ttttttacgt aaattataac ttttttgaaa
    50701 atgatttgta aacggattta tactatataa cttaaaatag acattttttg acaagatatt
    50761 tacaaaaatc cactagaaaa cagtaaattt tgtaaatgtt actggaaaga aacagatttc
    50821 tattgtttgt tttgtttaca aaaaagataa tgaaagtgta aaaaagtaaa ggagtgagaa
    50881 catggcaaaa ggaatcaaaa tcgctacgat tggtggaggc agcagttaca cccccgagtt
    50941 gatggaagga tttatcaaaa gatatgagga attaccgata cgagaaatct ggctcgtaga
    51001 tgtagaagaa ggaaaagaaa agcttgagat cgttagtcag atgtctcagc gtatgtggga
    51061 tgcttctccg tatgaagtca agatctatgc aactctggat cgggaagaag cattgaaaga
    51121 tgcggatttt gttacgactc agtttcgtgt cggtcaatta gatgcacgaa tcaaagacga
    51181 gcggatccct agttattatg ggatgattgg gcaagaaaca aatggtgctg gcgggatatt
    51241 caaagctttt cgtaccgttc cagtcgtttt ggcaatcgtt gaggacatga agcgtttatg
    51301 tccaaatgct tggctgatca atttcgcgaa cccaagtggg atggtgacag aagcagttat
    51361 cagatatggc aaatgggata aagtgattgg attatgcaat gtgccagtga tggcgatgat
    51421 gacagaacca gcaatgcttg gcaagaaaaa agaagagctg atctacaagt ttgccggtct
    51481 aaatcatttc cattggcaca aagtagcaga taataaagga aatgaccgca cggcagaatt
    51541 gattgatcgt ttatacgatg aagagaatgg attaccgaaa aatatctttg atatcccgtt
    51601 tttcaaagaa caattaaaac aaatgaacat gatcccttgt ggctaccacc gctattacta
    51661 tcgtgaagaa gaaatgctgg aacatgcttt agaagaatat caaacgattg gtacaagagc
    51721 gcagcaagtg aaagaaacag aagcagagct atttgaatta tacaaagatc cagaattaga
    51781 ccataaacct gaacaattgc aaaaacgagg tggcgcttat tattcagacg ctgcctgtga
    51841 aacgattgct tcaatctatg gaaataaaga aacacagatc gtggtatcta caaagaatga
    51901 tggagctgtt cctgacctgc cggcagattg tgtagtagaa gtcactgcct atgttggcgc
    51961 acaaggagcg agaaatgtag cgtttggttc tcttcctgct gctgaaagag gctggttgca
    52021 agtaatgaag aatatggaat tgctgacgat cgaagcagct gtgactggtg attatgggac
    52081 agctttacag gcgtttacga tcaatccact tatacgatca ggaaagacag caaaacggat
    52141 catggatgag ttgtttattg cacaccagca gtatttaccg caattcaagg agacaatcag
    52201 ccgattaaaa gaagaaggaa tcgaagttca agatgaactg gcaagagaat tatcaggtaa
    52261 tacacatata atggaggaag tataatggga ttcagaaaag attttttatg gggcggtgct
    52321 acagcagcca atcaatgtga gggcggttat gatgaaggtg gacgcggact agcaaacgta
    52381 gatgttgtgc caatcggaaa agaccgtttt cctgttatca ccggaaagaa aaaaatgttt
    52441 gaatttgatg aggatcattt ttatccagca aaaattgcaa tcgatatgta tcatcgttat
    52501 aaagaagata ttgctttgtt tgcagaaatg gggttcaaga cttatcgaat gtccatcgcc
    52561 tggagtcgta tttttccaat gggggatgag aaagagccga atgaagctgg cttgaaattt
    52621 tatgaggatg cctttaaaga atgccaaaag cacggaattg agccacttgt gacgattacg
    52681 cattttgatt gtccaatgca tttgatcaaa gaatatggtg cgtggcgttc cagagaactc
    52741 gttggttttt atgaaaatct gtgtcgagtc atcttcaatc gctataaagg acttgtcaaa
    52801 tattggctga cattcaatga gatcaatatg atccttcatg caccattcat gggcgcaggg
    52861 ctgtattttg aaaaaggaga aaatgaagaa caaatcaaat atcaagcggc acatcatgag
    52921 ttggttgcaa gtgccatcgc aacgaagatt gctcatgaag tagatccgga gaacaaagta
    52981 ggctgtatgc tggcagcagg agcatattat ccatacactt gcaatcctga agatgtttgg
    53041 gcttctagaa aatcagatcg agaaaattat ttcttcattg atgtccagtc acgaggagaa
    53101 tatcccgctt atgcattgaa agaaatggag cgaaatggca ttcagatcga aatggaacca
    53161 ggggatgccg cattgctgaa agaacatact gttgatttta tctctttttc ttattatgct
    53221 tcccgagttt cgactaccga caacgaactc ttggaacaga ctgcaggaaa tatctttgct
    53281 tctgtcaaaa atccatatct gcaagcaagt gaatgggggt ggcaaattga tccattaggc
    53341 ttgagaataa cgatgaatga tatttatgat cgttatcaaa aaccattgtt tattgtagaa
    53401 aacggcttag gtgcagtcga tacgccagat gaaaatggtt atgtggaaga tgattaccga
    53461 atcgattacc tggcagcgca tatccaggcg atgaaggatg cagtagaact agacggagtc
    53521 gatctattag gctatacgac ttggggatgt attgatttag tatcagctgg taccggtgag
    53581 atgaagaaaa ggtacggatt catttatgtc gatcgtgata accaaggaaa tggcacatta
    53641 gcccgttcaa agaaaaaatc attcaactgg tataaaaaag taattgattc aaatggagaa
    53701 aatctttcga acgattgatc aatgatttat aaaagtctat tgtttttcag cggatttttc
    53761 gagtataata aagctattcg agaaacgcaa gcagaacaat agactttttt tcgcattggc
    53821 tgtaaagtat gcttacttga ttaagtaaag gagaatctac atatgtatga tgtaatcgtt
    53881 attggcgcag gtcctgctgg gatgactgct gctttatatg cttcacgttc aaacttatcc
    53941 gtattgatga tcgaacaagg tgcccctggc ggccaaatga acaatacggc agaaatcgaa
    54001 aattatcctg gattcgattc gatcatgggc cccgatctag cttataagat gtatgaaagt
    54061 gtctcaagat ttgggacaga aaatgcttat ggaatcgttc aagatatcaa ggatcacggc
    54121 agccataaag aagtcatctg tgaagataaa agttacgaag gaaaaacagt cattatcgca
    54181 acaggatgta tccaccgtaa attaggcgta cctggcgagg aagaatttgc tggacgaggc
    54241 gtttcttact gtgcagtctg tgatggtgcg tttttccgta acaaacgtct agtagtcatc
    54301 gggggtgggg actcagcagt agaagaagcc atctatttga cacaatttgc ttctgaagta
    54361 gtgatcgtcc accgtagaga tgaattacgt gcacaaaaga tcatccaaga ccgtgcattt
    54421 gcaaatgaaa aaatttcctt tatgtgggat acagtcgttg acgaaatcgt tggaaacgaa
    54481 atggtagtga caggtgtgaa agcacgtaat gtcaaaaccg gcaatgaatt tgatttagca
    54541 gcagacggag tattcatcta tgttggactg gatccattaa ctgcaccttt tagagaagca
    54601 ggtttgacga acgaagctgg ctggattcca acagacaacg aaatgaaaac gaatatgcca
    54661 ggcgtttttg ccatcggaga tgttcgtgaa aaagatttgc gacaaatcac tacagctgtt
    54721 ggtgacggcg gtattgctgg acaacaagtg tataaatact tggagtcttt agaagaaaca
    54781 acagcataag tttaaaaaga gtagaagaac gggttcaatt gttcttttac tctttttttt
    54841 cgcgtgaaat ttactgaatc tgatttatgc gaacacgtcc aacacctttt gataaaagaa
    54901 tcctttcttg atttaagcgt tttttggtcc tcatactttc atatctaata agtgaatgct
    54961 atacttatta agaaccatat ttcagaaaga ggtgttattt tcatgtcttg ggaacaagtg
    55021 tatgaacaat gggtacatga agaaaattta gaagaaaact taaaaaaaca attaaatgat
    55081 ctgggtgaag atcctgaaaa acttgaagat gctttttatg caccgttgga attcggtact
    55141 gcaggtatgc ggggaatctt aggacctggg atcaatcgaa tgaacatcta tacggtaaga
    55201 caagcaacag aaggactagc acgtttcatg gatacacaag gtgaagaaac aaaacgccga
    55261 ggtgtcgcta tcgcttatga ctcacgccat cagtcaccag aatttgcaat ggaagcagcc
    55321 aaaacactag cacatcacaa tatcccgtct tatgtattcg aaagtctgcg tccaacacca
    55381 gagctttctt ttgcagttcg tcatttgaag acgtttgcgg gaatcatggt aaccgcatct
    55441 cacaacccag cggcttacaa tggatataaa gtctacggag aagatggcgg acaaatgcct
    55501 cctgcagacg cagatgcatt gacgaaatat gtccgtgaag tcagcaatcc gctaaaagtc
    55561 gaagtattat ctgacgaaga agctaaacat agcgggttga tcacgatcat cggagaagaa
    55621 gtggacgctg cttatctaga agaaatcaaa gcagtgacga ttgatcgtga attggttgaa
    55681 acaatgggga aagatttgaa acttgtgtac acgccacttc atggaacagg gaaaatgttg
    55741 ggcgaacgtg cgttgaaaca agctggattc gaacaattcg tactagttcc cgaacaagct
    55801 gtggcagatc cagatttcac tacggtcaaa tcgccaaatc cagaagaaca ttcagctttt
    55861 gaatatgcta tacgacttgg agaaaaagaa ggagctgact tgctgattgc aacagatcct
    55921 gatgcagacc gtttaggcgc agcggttcgt ttgccagatg gttcttatga agtattgact
    55981 gggaaccaaa tcggagcatt gtttgctcaa tatattttgg aagcacatca atcaagaggc
    56041 actttaccag aaaacgcagc ggtattgaaa tcaatcgttt ccagcgaatt gcctacagca
    56101 attgcggaac actacggtgc taccatgttc aatgtgttga cagggtttaa attcattgct
    56161 gaaaaaatcc aacaatttga agaagaccat tcacatactt ttatgtttgg attcgaagaa
    56221 agttatggtt atttagttaa accttttgtt cgcgataaag acgcgatcca agcattgctt
    56281 ttattagcag aagtagcagc ttactataaa aaacaaggca aaacgttgta tgacggcttg
    56341 caaacgattt ttgaaaccta tggctatttc gcagaaaaaa cgatttctgt gacaatgagc
    56401 ggacaagaag gttctgcgaa aatctctgct ttgatggaaa aattccgtaa agaagcaccg
    56461 acagaatttg ccggagcaaa agttattcaa acagaagact ttaaagaatt gacgagaaca
    56521 gaccgtgaag gaaacgtaga aaaactaaca acaccgccat ccgacgtatt gaagtataca
    56581 ttggaagaaa atggctggat cgctatccgc ccttcaggaa ctgagccgaa aatcaagttt
    56641 tatattggtg taaaaggaag ctctacagaa gacgcagccg aaaaagtagc ctcttatgaa
    56701 gcgattatca aaaagatgac agaagaataa aaaacttgag actgatggaa aaagtggtaa
    56761 actttagaag tcagtctttt ttcaagttgg aggtgaatgg attgccagac acgcaaaaat
    56821 caaaagtaga gaaagtcagt caattattca aaatgttgag tgatcccact cgtcttaaaa
    56881 ttctgctata tctgaaagac ggggaacaaa atgtgacagc aatcacccaa gcagtggaaa
    56941 tggagcagtc tgctgtatcg catcaactac gtttattaag agaaaatcat gtcgtcaagt
    57001 ctcatcgcga gggaaaagcg attttgtata gtcttgatga ttctcatgta ctggatattt
    57061 tgaaccagac actcaaacat gtggaacatc aataacgata ggtgaaaata gataaacact
    57121 ggagtgtgac aacgaagaaa tttcgttgtc acactccagt gttttttaag aaaaagacaa
    57181 aaatgattaa tcgctttttt ctgttgtttc agttccataa taactatgat cttctaggtc
    57241 taaagcattc aaaataagcg aagccgtttc ttcaatagat aaagatgcca cattgatcac
    57301 tacacatcct agtttttcat aaagatcatt cgcaaaatcg agttcagctc taattttttc
    57361 gatatcggaa taagcagtat cagggtttaa tccgtaagag cgcatccgtt ctttacggat
    57421 attatttaaa atatctgggt tattcgttag tcccacgatt tttttaggat cgatttccca
    57481 cagctgtttg ggaatatgtg cttggggaat caaaggtaaa ttagcaactt ttaaattttt
    57541 atttgctaaa aacaagctca aaggcgtttt ggaagttcgg gatacgccta gcaaaacgac
    57601 atctgcttct aaaaaaccac gaggatcttt cccatcatcg tattttacag caaattccat
    57661 tgctttgatc cgtttgaaat agttctcatt caataaatgg agggcaccag gttgccgaat
    57721 tggcttgata ccagttaaac gttcaatttc tgatatcggt ggcgtcatca catcgaaact
    57781 aaataattga tgttcttcac aaaaagaacg tgttatctcg atcagttctt gattgattaa
    57841 tgtatgcagg acaagtgctt tttcttctaa tgcatcttgc aatgcctgcc ttaatgtttt
    57901 ttcatctgtc acgaacgttc tgcggaatag gttgaactca acggtaggat actgagccat
    57961 agtagcttgc gctagttttg tcgcagtttc tccagcagag tcggagatga caaaaaaagt
    58021 aacgatttct gtagaatcgg aagacatgga taaaacctca cttgcatttt ttttatgttt
    58081 attataccat actctagaat gcgtacttcg taatcatttt gatttggagg aataagtcat
    58141 gaaaaaacag acattgatcg aacaatcatt agaaacattg aaaaataacg gttttaaata
    58201 tactaaaaaa agagaagcat tgttaactta tttagtgaag cgtaaccgct acgtatctgc
    58261 taaagaagtt tttgactata tgaatcagga attcaaaggg gtaagttatg atacgattta
    58321 ccggaatctc catgattttg cagagatcga tttactggaa gaaaccgatt taaatggtga
    58381 aatgaagttt cgttttcatt gctgtcaggg ggaaatcggc caacaccatc attttatctg
    58441 tacagtgtgc ggaacaacga aagaaatcca tatgtgtcca atgaattatt tcgaagaaca
    58501 gctaggtgga tgcacgatcg aagggcatag attcgagatt ttcggaagat gtgaaaattg
    58561 tcaataagga tttaaataaa ataacatttg ataatttaaa aaaaaagcaa gatttccttg
    58621 acggtcaaaa cagcattaga tataatattt acaggacagt cttgtttttt gagatatatt
    58681 tcaataacaa ttacggttac tttttaagct cggagggagg gaattaacat gtcaaaaaca
    58741 gtcgttcgca aaaacgaatc acttgacgat gctcttcgtc gcttcaaacg ttccgtgtca
    58801 aaagcgggta ctttacaaga atctcgtaaa cgtgaattct acgaaaaacc aagtgtaaga
    58861 cgcaagaaaa aatctgaagc agctagaaaa cgcaaaaaat tctagttctg tgatgaattg
    58921 gagttggtag tgtgtcacta ctaagtagat taaacgatga catgaaagca gcgatgaaag
    58981 cgaaagataa ggaatcttta caagtcatcc gaatgatcaa gtcatctata caaaacgagc
    59041 agattaaaga aggtcacgac ttgacagaag aagaagaact aacggtgttg tctcgcgaaa
    59101 tgaaacagcg acgtgattct ttgcatgagt ttgaagaagc aggacgtgac gatttagctg
    59161 aaaaagtaaa aagtgaaatt gtaatagtcg aaaaatatat gccggaacaa ctttccgacg
    59221 aagaaattcg acaacttgtt caagaagcaa tcactcaaac tggtgcttca tcaatgaaag
    59281 aatttggcaa agttatgggt gcaattatgc ctaaagtcaa aggaaaagca gatggaaacc
    59341 aagtgaatgc gattgtgaaa gaactattac aatcttaatt ttatcattta aactgcggac
    59401 agtgattgtc cgcagttttt tgtgttcgta aaaaaagaac taaaaaacag ctagaagaaa
    59461 gaataagatt ctttcttcta gctgtttttt tcctgtttaa tatctcttat gttaaattgt
    59521 gtttgatttg gttattcttc tggtagttca tttgcttctt gtttataccc tgataggcga
    59581 taggcgtaat aaaagagtaa gctgccagcg taaccaagta acgttgggac catccagcca
    59641 agtcctaatg cagcaaaagg aacgtgatga tgataaaatc ctagtaagct tgctacgaca
    59701 gtttgctgac tcactaatgc aggcgaattt gccaacatat ccaatacggc tggaacggca
    59761 gaaaagaaca tcgttacttg atagacaaat ggactctttt ggaagaattt tgctgttaag
    59821 gaaagcaaga ttaacgcaag tgacaatgga tacaaaagca tcaatactgg taaagaccat
    59881 tggatgatat tatccaatcc ggcattcgca acgacaaaag agacaaaagt cgttaaccgc
    59941 agccatgtca tataactgac tttagggaac agtttatgga aatcttgtgc aaaagaaaca
    60001 acgagtccca tagcggtagt gaagacacct aatgtgacca ttacccctaa gaagctagca
    60061 ccgaagttac ctaaataaaa attagtgatc tgagaaagtg cagttccgcc attttctgat
    60121 aaaggcatct tattcaaact catcgcaccg agaaggacca gaccaaaata aatcatgact
    60181 tctaacgcaa cgctcaggct accagctttt gctgttaatt ctgtgacttt tttatcttta
    60241 aaaccaagat cacgaactgc atggacaaaa gtgacactta aagcaagtaa agctactgca
    60301 tcaaccgtgt tgtatccttc taaaacgcca tttgtcaaag cgttcgtctg ataagaatct
    60361 gcagcagatt ggtttagatt ccccatgggt tggaataaag aaacgacaaa aatgatagct
    60421 aataatgcaa gaaacaagga gtttaaatac tttccgacat atttgaccaa acgtgttgga
    60481 tgtacagtaa ggaaataagc aagtaaaaag aaaagagcag agaataaaaa catacctaat
    60541 gaatcatatt tttcaggcaa aaagggttta gcagccattt caaaagcagt tgctgctgtc
    60601 cgcggtgtac caaagaatgg tccgatcgtt aaatgaacaa ggatcaagaa caaggcagca
    60661 taccattttc cgacaggttt agctaagtca tacagaccat cactttttgt cactactaca
    60721 gctaagatag ctaataatgg gaaaagagcg cctgagatgg caaatcctac accagcgctg
    60781 atccagtttg atccagccat ttgacctaag tggacaggaa agatcagatt acctgacccg
    60841 aagaacatcc cgaagataag tgaactgacc atcaataatt gcttccatga ataagttttt
    60901 gtttcttcca taaatttttc ctccaataat tatcattttg agtaacaaaa taaacgttgc
    60961 agaaactaga aatgccacta atatcagatt ccatgaaaac caccacctta aaatataaaa
    61021 aaaagccctt ttccacttgg gaaaagggct ctagattcta gaacggtacc accttttttc
    61081 gcttaaaaaa gcttcttcat tcacggacgc ttttcgataa agttatccgc tagcctgata
    61141 atggaggcag ccacaatgat ctacgttttc gacattgact cggaaatgat gttcgtctat
    61201 tcgatttatt gtcatttctc acttacatga ctcactggga ataaatcgct gaatagccta
    61261 ctgttttcct cgacgctttg ctgtatattt atttgttgac gttataatac acctgttttt
    61321 tttgaaatgc aagaatattt caaatatttt gttttctttt tttgaaacaa gaaaaatacc
    61381 cagagaatac ttgctaagtt agacggaata cttgtcctct atcttatgca tagcttacat
    61441 gatcagcttt ttttattcaa acgagacctt tagagaatgg aagcagctat tttttaacgt
    61501 ttcttggtac ttatggttaa gcaactcttt tagaaccttt tcttatttgc tatatagttt
    61561 cttctgagta tggtaaaata ggaaagaatg aatcgaaaaa aaggggatgt ctatttttga
    61621 cggaagagtc tagttcttta gagattagat tgacggctgc tgatgatgtc agcatgcttt
    61681 taggtgcaca cgacaagcat ataaaactga ttgaagaaac aacagaaaca accatccata
    61741 cacgtggaga aatcattcag atcattggcg aacaatccca gatttccttg gctgcctcag
    61801 tcattcggac attgcaggaa ttgatcaaac gaggaatcca tgtaagtaca ccagatgtcg
    61861 tgacggcact gaaaatgggg caaaaaggca cattagatta ttttgttgaa atgtatgagg
    61921 aagagatcgt caaagaccgt aatggaaaac caatacgggt aaaaaacagc ggccaaaaga
    61981 aatatgtcga atcggtagcc aaacatgata tcgtatttgg cgtaggacca gccgggacag
    62041 gaaaaacttt tcttgcagta gtattagcaa tcgctgcctt gaaaaaaggt gaagtgcaaa
    62101 aaatcatttt gacgcggcct gctgtggaag caggggaaaa tctaggcttt cttcccgggg
    62161 atctaaaaga aaaagtagat ccttatctgc gtcctgttta tgatgcttta taccaaattt
    62221 ttggattaga tcatacgaac cgattgatgg aacgaggcgt gatcgaaatt gctcctttag
    62281 cttacatgcg ggggcggaca ttggatgatg cattcgttat tttggatgaa gcacagaaca
    62341 cgacagttgc tcagatgaaa atgttcctga cacgtcttgg ttatcaatca aaaatgattg
    62401 tcaatggaga tacgagtcag attgatctgc ctcgagggac aacaagtggt cttgttcatg
    62461 cagaacgaac attaaagcaa atcaagaaaa tcgattttgt taattttgaa gcaagtgacg
    62521 ttgttcggca ccctgtagtt gccgaaatca tcaaagctta tgaaaaggca gatctccatc
    62581 aagaataagc tttgcttagg aggataagca catgttgaat aaaacactgc agaaactaca
    62641 agaaaaactt ggaaaggcag cattgccttt cctgctgttc ttgtttttta tcattatctg
    62701 cggcgttaca tttagtagtg tcaaacaaaa ggccaatgat ttcagagaag gccaagtagc
    62761 agaagaaagt atccgtgcga acaaaacgat cgagaacacg gaagaaaccg aacaaaaaag
    62821 aaaactggca gcagaagcag tcactccaga atacacttac caaaaagatc ttgcagatga
    62881 ccagaacaat cggatcaagc aactatttga attgatcgat aaaacgaatg atgccatcaa
    62941 caaatcttat gacgaaaaga ttgataaggc aaaggaagat gaaaatatcc caaagcctac
    63001 tacagaagag cggatcgccg cgttgaagaa aaattttgaa aatgtgaatg cagaaaatgt
    63061 tgcgttttat caaaaactac cgaacaattt ttattcgacg atatttgaaa tgactgaaac
    63121 tgagatccat actgtgaggg atgagagtct gaagctcatt gacgaacaga tgagcaaaca
    63181 agttcgtgaa agtgaattgg aagcttttaa acaagaagca gaagagcaaa tccaatattt
    63241 gaatgtcaca ccagcacaac aacaaatgat tcgctactta gtggatcaag gtatcgtagt
    63301 gaatgacgta ttgaatgaga aaaagacaga agaattgaaa caatccgcac gagaagctgt
    63361 acaaccagtg atgatcttcc aaggagaaat cattgttcgt gaaggtaatc aaatcgatgc
    63421 ttcagctatg aaaaaattgg aattactagg actgactaat caaacaactt cgatttttcc
    63481 gctaattgcc atggttttag ctgttttgct tcaaatcggc gtactgattt ataattcact
    63541 tcaattccaa aatgcaggag aaagaacaaa atacgtgctt ttctatgtga cagctatgtc
    63601 agtcagcata gtgctgatga aatttttcca attattccaa acggaacaag ctacttatat
    63661 cccgctgttt tatcccgctg cttttgcacc acttgtttta agctttttct tgaaccgaag
    63721 agcagggatc atggctggta ttttccaagc agtatctgca cttttcattt tttatgattc
    63781 gattggcacg aactttctaa cgattatctt gatgtcttat gttttctctg ggctcatggc
    63841 aacgattgtc agacgcaaac ggatctcaga acaaggatta tctgctgcca tgtgggtcat
    63901 tatttttcct gtgatgatgg atgtcatttt gatcgtctat caaggaatga gctttggtga
    63961 tgcaacgact tggttaatgc ttgtctgcgg gttcactggt gcactcttct cattcttgct
    64021 gacgatcggt ctacacccat atattgaatt gatggtcaat gatgacagtg taattgtatt
    64081 aaatgaactc agcaatccaa atcatccttt attgaaacag ttgctggaag aagcgccagg
    64141 tacttatcat catagtatga tggtagctaa tctaagtgct aacgctgtag cagaaatcgg
    64201 cggacgttct ttgctaactc gagtagcttg ctattatcat gatatcggca agatcaaaca
    64261 tgctagcttt ttcgttgaga acttgccttc aggagcggaa aacccgcaca atttcttatt
    64321 gccagaagac agtaaacata ttatctttgg acatgtgaca gatggagcca aaatattgga
    64381 agattataat atgccgcaaa tggtgatcga tatttgcaaa cagcatcatg gaaccacgct
    64441 aatgcgttat ttctacatca aagcgaaaga acgcaatccg gaagtaacag aagagcaatt
    64501 ccgttatcct ggaccaaaac cgcaaagtcg tgaagcaggg atcgtcaata ttgctgacag
    64561 ttgtgaggct gcagtacggg caatggatca tccgacgagc gaaaaaatcg aaagttttgt
    64621 ccataatctt atcgaagagc gtatttccga tggtcaatta gacgatagtg gactgacctt
    64681 gaaagaaatc cgaaaagtgg aaaaatcatt gatcagcggt ttaagtagca ccttccattc
    64741 tcggatcaaa tatcctaaaa tgaaaacaga agttgagaag ataaaggaag aacaagaaga
    64801 atagaaagaa gcgatgtata atggatatta cctttatgga tgaaacaaac caagtctcag
    64861 aagagaaaat gaaagaaatc gatgacttgc tgcaatttgc agcatcttat ttaaagctgc
    64921 cagaggaaac agaaatgtct gttactttca tggacaatgc ggcaatccaa gtaatcaatc
    64981 gtgattaccg tggaaaagat gcaccgacag acgtcatcag ttttgctctg gaagaagaag
    65041 gcgaagacga aatcccagtg atttttgaag atggagagaa tccattgcct cgtgtactag
    65101 gagacatcat gatttctatt gaacgtgctg ctgaacaagc agaagcttac ggccattcat
    65161 atgatcggga acttggcttt ttagcggtac atggattctt gcatatcaac gggtatgacc
    65221 atatgacacc agaagatgag aaagaaatgt ttggtttaca gaaagagatc ttggatgcct
    65281 atggactcaa aagataaaaa cgtagagaaa aacaaacatt tcctgatgtc tgtggaattt
    65341 gctgtccaag gagtcaaaac cgtttttgag gaagaacgca atatgaggaa acatgttgcg
    65401 ttcggtatcc ttgctttatt tatgggcgtg attttccagc ttgatcggat agagtggtta
    65461 tggctgcttt tagctgtttt tctcgtttgg atagtagaga tcatcaatac tgtttttgaa
    65521 aatgtagtgg acatgtttac ggattttcat tttcatccga ttgggaaaaa aatcaaagat
    65581 atggcagctg gagcagtttt agtgaccgct tgttttgctg tcatcattgg gttgatttta
    65641 tttgtcccta aaatatatca tttgttgttt aattaaaaag gagaacgtat atgccagaac
    65701 ataaatcagg gtttgtcgca atcgtcggcc gcccgaatgt aggaaaatcc acattattga
    65761 accgaatcgt gggtcaaaaa attgccatta tgagtgacaa ggcacagaca actagaaata
    65821 aaatccaagg agtatatact acaccagaag ctcagctgat ttttatcgat acaccaggaa
    65881 tccataaacc aaagcatcgt ctaggagatt ttatggtaga agcagcttac agtgcgttga
    65941 aagaagtgga tgcagtctta tttatgatca gtgctgatca aaaacgcggc cggggagacg
    66001 attttattat tgaacgtcta aaaaacgtgc attctccagt ttatctgatt atcaataaaa
    66061 tcgataagat ccatccagac gagttgctgg gaatcatcga agattattcg acgcagatgc
    66121 cttttgcaca agtgattccg atctctgcta cagaaggaaa caatgtcgaa cgcttaatgg
    66181 atgtattagt cagtgaaatg ccagagggcc cacaatattt tccagatgat caagtgactg
    66241 atcatcctga atactttatc gtttctgaat tgatccgtga gaaagtcttg tttttaacac
    66301 gagacgaagt gcctcattct gtcgcagtcg ttgttgattc gatgaagcga aacgagaatg
    66361 ataaaatcca tatccaggcc acgatcatcg tcgaaagaga tagccaaaaa ggaatcatta
    66421 tcggcaaagg cggaaaaatg ctgaagcaag taggaacgaa agcaagaaag gatatcgaaa
    66481 atcttttagg agacaaagta tttctagaac tttgggtcaa agtccaaaaa gactggcgag
    66541 acaaaaaagt ctatctgcaa gactttggct accgaaaaga cgaatactga tattctgaac
    66601 ttcttatagg ttaagtatag aaataaactg gataaaaatc cgcacgttga gaaaaacggc
    66661 ggaagatcga aacataaata atctggatcg gcagaatctg cttttccaga tttatttttt
    66721 aggagggaga atatgagcca gcttgcagaa tcgaaaggct tgattttatc tagccgtaat
    66781 tacaaagaaa aagataaatt agtcaaaatc tttacggaat cttctggcaa aatgatgttt
    66841 tatatcaaag gaatccatcg gcaaaatcag ccattagctc cggctttgct cccctttacc
    66901 gaagcagtct atatcggtaa ttttcgaagt gaaggtttgt cgtttttaag cagtgtcaaa
    66961 gacgtccatg cgtttcggaa catccaagaa gatatctttt tgagcggtta tggtacctat
    67021 ttgctaaatc tagtagatgc agcaatcgaa gatcatcaat atgacccgca tttattccaa
    67081 tttacgcaac aagctctgca gcggatggat caaggagatg atgcagaaat catcacgaat
    67141 atctttgaag ttcaaatact gcaacgcttc ggaatcgcgc caatttggac gcactgtgtc
    67201 gtatgcgggg aaacaaaagg aaaatttgac tattcttcca aatctggcgg tgtgatatgc
    67261 gagaaacact ggccaatgga cgaacaccgt tatcatgcag atcctcgcgc tgtttatttc
    67321 gttcgaatgt tttcggctat ttcttatgat aagatcagtg gaatccactt gaaagaagaa
    67381 acgaagcagg caatccgtga cctcattgac cagttgtatg atgaatatgt cggtattcat
    67441 ctaaaaagca aaaagttcat tgatcaaatg aagtcttggg cacacgtatt aaagacgcca
    67501 gagaaaaata tgcctgaaaa agagatacct gaaaaagaag aataaatgaa agatacaaca
    67561 aaaaaatcaa aactatttga caaatacagg gacttccgtc tattattaac cgtagattaa
    67621 atagatcgtt cactgagagg atctattata taaacaagaa aactgcaaaa tgaatacgta
    67681 cattgaccaa gatcagtaaa aaacacgtgg tttagcgagt tcgggagagt ggaagccgga
    67741 cccaaacgat ttttgaagag gcacttgcga gtacgataaa tgaagctgcc gaatctttat
    67801 tcggaagtag ggtggaaccg cg
//
LOCUS       DACDKM010000006        60192 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20834_2016
            SAMN12640370-rid9891663.denovo.006, whole genome shotgun sequence.
ACCESSION   DACDKM010000006 DACDKM010000000
VERSION     DACDKM010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640370
            Sequence Read Archive: SRR10040891
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 60192)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 60192)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742835.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742835.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:04:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,012
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 29 of 75
            Pseudo Genes (incomplete)         :: 33 of 75
            Pseudo Genes (internal stop)      :: 19 of 75
            Pseudo Genes (multiple problems)  :: 5 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..60192
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640370-rid9891663.denovo.006"
                     /isolate="E20834_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(114..473)
                     /gene="rplT"
                     /locus_tag="IV334_000388"
     CDS             complement(114..473)
                     /gene="rplT"
                     /locus_tag="IV334_000388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HAP8942486.1"
                     /translation="MARVKGGTVTRKRRKRVLKLAKGYYGSKHTLFKTAKEQVMNSYN
                     YAYRDRRQKKRDFRKLWIARINAAARMNGLSYSKLMHGLKVAEIDINRKMLADLAVND
                     AAAFTALADQAKEALAK"
     gene            complement(526..726)
                     /gene="rpmI"
                     /locus_tag="IV334_000389"
     CDS             complement(526..726)
                     /gene="rpmI"
                     /locus_tag="IV334_000389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001125942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HAP8942487.1"
                     /translation="MPKQKTHRGSAKRFKRTGKGGLKRFRAFTSHRFHGKTKKQRRQL
                     RKASMVSSGDFKRIRQQLAKMK"
     gene            complement(798..1322)
                     /gene="infC"
                     /locus_tag="IV334_000390"
     CDS             complement(798..1322)
                     /gene="infC"
                     /locus_tag="IV334_000390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HAP8942488.1"
                     /translation="MTIAKDMMVNDGIRARELRLIGSDGEQLGVKTKAEALNIAEQAN
                     LDLVLVAPGAKPPVARIMDYGKYRFEQQKKDREARKKQKVINVKEVRLSPTIDLNDFN
                     TKLRNARKFLEKGDKVKASIRFKGRAITHKEIGQKVLNRLAEETADIATVEQKAKMDG
                     RSMFLTLAPKNDSK"
     gene            1642..2274
                     /locus_tag="IV334_000391"
     CDS             1642..2274
                     /locus_tag="IV334_000391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HAP8942489.1"
                     /translation="MKKIEPIPPSLPLLTKGEFYLEDEEIFSGLLINGGDQSYLDAKN
                     MVLRESHIQKLTMQKTVLRHFECSNVIFEKCDFSNLEWIGGSFHQVVFHQCKLTGTNF
                     AESYLRDCTFTDCIASMASFSSTNLKAVSFDHCQLEDSEFYEVTWKNLFLSDNQLTGS
                     NWFRTSLKGLDFTTNFFTAIALSQDSLAGLIVNQEQALVIAQALGLQIKD"
     gene            complement(2276..3181)
                     /locus_tag="IV334_000392"
     CDS             complement(2276..3181)
                     /locus_tag="IV334_000392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HAP8942490.1"
                     /translation="MREKHYFNQDRNFWINVQQNDVLAVKNLKEEYGISDEMLTYSLD
                     KNERARVEYDSFDEALLLVSNVPHQQKVDNHYETSPIAFILKEDGLFTFTTPNTEYVI
                     RLIRSLLERMPDMSVYSLLFRTLFLISDSFFPLIEEVNSERQRLNLKLREKTTNKNLL
                     QLSDLEIGLVYLVTGTKQNVVLLEQIKALAIYRKLSEKEKEELDDALIEARQAVEMTN
                     LASQILDQLSGTYNNLLNNNLNDTMKFLTVWSLILTVPTIVTGFFGMNLQLPFTHSVF
                     GWGIALIISLVLSIWMLIALWRRIR"
     gene            complement(3266..3919)
                     /locus_tag="IV334_000393"
     CDS             complement(3266..3919)
                     /locus_tag="IV334_000393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HAP8942491.1"
                     /translation="MIVLAGTIGAGKSSLTEMMAEHFDSQAFYESIDDNEVLPLFYAN
                     PEQYAFLLQIYFLNKRFASIKQAMKDDNNVLDRSIYEDSLLFHLNADLGRATETEVRV
                     YDELLENMMEELPYAAHKKHPDLLVHIRVSFDTMLERIEKRGRSYEQLSFDPSLYDYY
                     KELNRRYDQWYEEYKESPKIQIDGDRYNFVEDPQAKEEVLKMIEEKLAEIRQTKVAI"
     gene            4241..5185
                     /gene="mvk"
                     /locus_tag="IV334_000394"
     CDS             4241..5185
                     /gene="mvk"
                     /locus_tag="IV334_000394"
                     /EC_number="2.7.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mevalonate kinase"
                     /protein_id="HAP8942492.1"
                     /translation="MANYGQGESSGKIILMGEHAVVYGEPAIAFPFYATKVTAFLEEL
                     DAMDDQLVSSYYSGNLAEAPHALKNIKKLFIHLKKQHDIQKNLQLTIESTIPAERGMG
                     SSAAVATAVTRAFYDYLAFPLSREILLENVQLSEKIAHGNPSGIDAAATSSLQPIYFT
                     KGHPFDYFSLNIDAFLIVADTGIKGQTREAVKDVAHLFETQPHETGQMIQKLGYLTKQ
                     AKQAIIENSPETLAQTMDESQSLLEKLTISNDFLNLLIQTAKDTGALGAKLTGGGRGG
                     CMIALAQTKTKAQEISQALEDAGAAETWIQGLGVHTYV"
     gene            5178..6155
                     /gene="mvaD"
                     /locus_tag="IV334_000395"
     CDS             5178..6155
                     /gene="mvaD"
                     /locus_tag="IV334_000395"
                     /EC_number="4.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diphosphomevalonate decarboxylase"
                     /protein_id="HAP8942493.1"
                     /translation="MFKGKARAYTNIALIKYWGKKNEELILPMNNSLSLTLDAFYTET
                     EVIFSDSYMVDEFYLDGTLQDEKATKKVSQFLDLFRKEAGLSLKASVISQNFVPTAAG
                     LASSASGLAALAGACNTALKLGLDDLSLSRFARRGSGSACRSIFGGFVEWEKGHDDLS
                     SYAKPVPSDSFEDDLAMVFVLINDQKKEVSSRNGMRRTVETSSFYQGWLDSVEGDLYQ
                     LKQAIKTKDFQLLGETMERNGLKMHGTTLAAQPPFTYWSPDSLKAMDAVRQLRKQGIP
                     CYFTMDAGPNVKVLVENSHLSEVQETFTKLFSKEQVITAHAGPGIAIIE"
     gene            6168..7253
                     /locus_tag="IV334_000396"
     CDS             6168..7253
                     /locus_tag="IV334_000396"
                     /EC_number="2.7.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomevalonate kinase"
                     /protein_id="HAP8942494.1"
                     /translation="MIEVSAPGKLYIAGEYAVVETGHPAVIAAVDQFVTVTVESARKV
                     GSIQSAQYSGMPVRWTRRNGELVLDIRENPFHYILAAIRLTEKYAQEKNILLSFYDLK
                     VTSELDSSNGRKYGLGSSGAVTVATVKALNVFYALNLSQLEIFKIAALANLAVQDNGS
                     CGDIAASCYGGWIAFSTFDHPWLQEQETQHSISELLALDWPGLSIEPLIAPEDLRLLI
                     GWTGSPASTSDLVDQVHRSREDKMVAYQLFLKNSTECVNEMIKGFKENNVTLIQQMIR
                     KNRQLLHDLSAITGVVIETPALNKLCNLAEQYEGAAKSSGAGGGDCGIVIVDQKSGIL
                     PLMSAWEKAEITPLPLHVYSDQRKENR"
     gene            7250..8314
                     /locus_tag="IV334_000397"
     CDS             7250..8314
                     /locus_tag="IV334_000397"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 2 isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HAP8942495.1"
                     /translation="MNRKDEHVSLAKAFHDKQKNEFDFVRIIHNPLPQIAVSDVDLST
                     QAVGFTLSSPFYINAMTGGSEKTKKINQDLAIVAREADLMIATGSVSAALKVPSLADT
                     YTIMRQEYPHGKIIANIGAGTSVERAKEAIRLFHADALQIHLNAPQELVMPEGDRDFT
                     NWKVLIQETQTAIDVPLIVKEVGFGMTRETLNDLAALGVHTVDISGRSGTSFTQIENA
                     RRSKRELSYLADWGQSTVSSLLEANEADTSMEILASGGIRNAYDIFKALCLGANAVGT
                     SGTVLTHLMNHGVEETIILMKQWQEELRLLYTMVGATNTAALHQQSLIFSGPVKDWCE
                     ARGIDLVKYGRRTEKSVGQK"
     gene            complement(8563..9366)
                     /locus_tag="IV334_000398"
     CDS             complement(8563..9366)
                     /locus_tag="IV334_000398"
                     /EC_number="1.1.1.69"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate 5-dehydrogenase"
                     /protein_id="HAP8942496.1"
                     /translation="MEFSMDMFRLDGKVALVTGAVYGIGFEIARSLATAGAKIVFNNL
                     NQESVDEGIAHYKEAGIDAKGYVCDVTDEEAVQAMVQQIKEDVGSVDILVNNAGIIKR
                     TPMIEMDAADFRQVIDVDLNAPFIVSKAVIPDMIEKGGGKIINICSMMSELGRETVSA
                     YAAAKGGLKMLTKNIASEYGQYNIQCNGIGPGYIATPQTAPLRETQENGERHPFDQFI
                     VGRTPAARWGEPIDLAGPSVFLASSASDFVNGHVLYVDGGILAYIGKQP"
     gene            complement(9386..10228)
                     /gene="kduI"
                     /locus_tag="IV334_000399"
     CDS             complement(9386..10228)
                     /gene="kduI"
                     /locus_tag="IV334_000399"
                     /EC_number="5.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-dehydro-4-deoxy-D-glucuronate isomerase"
                     /protein_id="HAP8942497.1"
                     /translation="MQEMETRYTHSPEDIRHYSTEQLRKEFLVEKVFEPGKISLTYTH
                     NDRMIFGGVTPTEKELEITLDKELGVDYFLERRELGVINIGGPGFIEIDGQKEEMKKQ
                     DGYYIGKETRHVVFSSQDAENPAKFYISSVPAHHKYPNVKISIDQVKPMETGEGLTLN
                     ERKIYQYIHPNVCESCQLQMGYTILEPGSAWNTMPCHTHERRMEAYVYFDYANEDTRV
                     FHMMGKPDETKHLVVDNEQAIISPSWSIHSGVGTSNYSFIWAMCGENITYTDMDMVQM
                     DQLK"
     gene            complement(10244..11251)
                     /locus_tag="IV334_000400"
     CDS             complement(10244..11251)
                     /locus_tag="IV334_000400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HAP8942498.1"
                     /translation="MGKVVTLGEIMLRLSTEQGTRIDSSERFAAHYGGGEANVAISLA
                     NYGHEAIFATKVPDNALGKAVKKHLQCYGVDTKQVLSGGSRLGTYYMETGSGERAASV
                     IYDRAGSSFAEMDHLEWSLEELFQGIDIFHVSGITPALSKKWQKMTKQVIEAAKKAGC
                     LISFDINYRGKLWTQQEASEVIHQLLPLVDICSAGKMDALYLLGIEKAPEAEEQPLIY
                     YYQKMQEKFPNIQVFYSTKRTVHSASENELKGTLWMNHEYVESQVHRITPIVDRVGGG
                     DAFAGGVLHGILSNMSPQEIIDFATAASALKHTIHGDCNQFSQEEVAQFLACGSGKII
                     R"
     gene            complement(11258..11902)
                     /locus_tag="IV334_000401"
     CDS             complement(11258..11902)
                     /locus_tag="IV334_000401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HAP8942499.1"
                     /translation="MKKIDILSRLKNAGVIAVVRGKSKEEALNACHAIIKGGLTGIEL
                     TFTVPQADQVIKELLSFYKDQPEIVIGAGTVLDAVTARLAILAGAEYIVSPSFDQETA
                     EMCNLYQIPYLPGCMTITEIKTALKSGVDIVKLFPGSAYGPSIISAFKAPMPQVNIMP
                     TGGVSLDNMKEWFDAGVVTVGVGGNLLAPAATGDFDKVTEVAQQYAAKMKEIKR"
     gene            complement(12002..12760)
                     /locus_tag="IV334_000402"
     CDS             complement(12002..12760)
                     /locus_tag="IV334_000402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HAP8942500.1"
                     /translation="MENKKPYGTVLIKASKILDYLAENQDVSLQTIAKGVGMTSSTVL
                     KILDTLLMIGYVNKNSEKNYRLGAKLIRYANKNIEQIDLVELTLPFLEQLQQKIDETI
                     HLGVLDNNEILYVNKLDPKHQTIRMSSKIGITRPLYNSAMGKAVLAEFSEEQYTDYLE
                     KHPLIPYTEYTITNALRLKKEIKTVKETHIAFDDEEIERDIFCIGASIVKNEQIIGAF
                     SVSMPKYRLTDETKEQIISALKQTKSEIEQRIQK"
     gene            complement(12887..14038)
                     /gene="fucO"
                     /locus_tag="IV334_000403"
     CDS             complement(12887..14038)
                     /gene="fucO"
                     /locus_tag="IV334_000403"
                     /EC_number="1.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactaldehyde reductase"
                     /protein_id="HAP8942501.1"
                     /translation="MSNRMILNETSYFGKGAIESIVPEFQQRGFGKAAVITDKGLIEH
                     GIATKVTELLDKAAIAYALYDGIVPNPTIQNVKDGVAFVKEAEADCLIAIGGGSPIDT
                     AKAIGIILTNPEFSDVISLEGVADTKNPCLPILAIPTTSGTAAEVTINYVITDEVNHR
                     KFVCVDPHDIPIVAFIDSDMMMGMPKKLAASTGMDAMTHAIEGFITKGAWEMTDMLHL
                     KAIEIIGHSLEASVDGDQNGREKMALGQYIAGMGFSNVGLGLVHGMAHPLSAWYNIPH
                     GVACAALLPTVMKYNKEYTGEKYREIALVLGIKGAAEMSLEDVREAACGEIDRLSKAV
                     GIPETISELGVKEADIPAIAEDALRDVCTPGNPRETTVEEIIALYQSLM"
     gene            complement(14120..14431)
                     /gene="rhaM"
                     /locus_tag="IV334_000404"
     CDS             complement(14120..14431)
                     /gene="rhaM"
                     /locus_tag="IV334_000404"
                     /EC_number="5.1.3.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose mutarotase"
                     /protein_id="HAP8942502.1"
                     /translation="MVKKAFCMKVYPEKQEEYKKRHDELWPEMRTALREHGVIDYSIY
                     LDPSTSLLFGYLVIEDEKLWEKMANTSINQKWWKYMEDIMETNPDASPVSWELMPVFE
                     L"
     gene            complement(14433..15278)
                     /gene="rhaD"
                     /locus_tag="IV334_000405"
     CDS             complement(14433..15278)
                     /gene="rhaD"
                     /locus_tag="IV334_000405"
                     /EC_number="4.1.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulose-1-phosphate aldolase"
                     /protein_id="HAP8942503.1"
                     /translation="MKKIDILQAPYVKEMIETTSNLYRLGWDERNGGNISYLLKEEEI
                     TPFLDPEKVIRRISMIFDASRLAGCYFIVTGSGRYFKNVASEPAENLGIVRVAEDGKT
                     LELLWGLENECLPTSELPSHFMSHIARLEVDPDNRIVMHCHASHLLAMSFTHELDERS
                     FSRTLWQMCTECLVVFPEGISIIPWMVPGTNEIGEATAEKMKETRLVLWPQHGIYGSG
                     KDMDEVFGLIETAEKAAEVYTYVKAQGPILQTITDENLWHLADAFGVTPKAGYLEEVH
                     TKAGV"
     gene            complement(15292..16548)
                     /gene="rhaA"
                     /locus_tag="IV334_000406"
     CDS             complement(15292..16548)
                     /gene="rhaA"
                     /locus_tag="IV334_000406"
                     /EC_number="5.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose isomerase"
                     /protein_id="HAP8942504.1"
                     /translation="MNTIERNYEQAKEKYATIGVDTDAVLEKIQDIKISMHCWQGDDV
                     KGFLTPDGELTGGIMATGNFPGAARTPEELRQDLEKAYSLIPGKHKLNLHAIYLDTEE
                     SVDLNEIEPKHFEKWVEWAKKEEIGLDFNPTFFSHPMMKDGFTLAHPDKEVREYWIEH
                     GKRSRKIAEYFGKETGQTSINNFWVPDGMKDNPIDRFTPRERLMESLDEIFTEELDER
                     YVQDAVESKLFGLGAEAYTVGSHEFYMGYGLTRDKLVCLDAGHFHPTEVISNKLSSLA
                     LFSKGIMLHVSRPVRWDSDHVVLMDDELQEIAKELVRNELLEKTNIGLDFFDATINRI
                     AAWVIGTRNTQKALLKAMLEPVERLKEMELAFDFTSRMAYTEELKDFPYADVWNYFCY
                     KNQVPVGLDWLEEVQEYQKDVLELRK"
     gene            complement(16545..18005)
                     /gene="rhaB"
                     /locus_tag="IV334_000407"
     CDS             complement(16545..18005)
                     /gene="rhaB"
                     /locus_tag="IV334_000407"
                     /EC_number="2.7.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulokinase"
                     /protein_id="HAP8942505.1"
                     /translation="MNPTFVAIDIGASSGRLMKSTRDAKGSLILEEVHRFKNGFRNKD
                     GHDRWKIDYLIKEILIGFEKLKQTGIDRLFAGIDTWAVDYCLIDEQGDRINDPIAYRD
                     KRTNGAVEQFSEIYSLDKLYEQTGIQIQPFNTLFQLFAEEKENLAKAHKLLLIPDYLG
                     YVFTGNMVTEKTNASTMQLLNAGTKEWEIELLEKIGVSKELFPPLTEPGTILGDLQKE
                     QFSKYDLPDVTFITVASHDTASAVLGTPGQGERWGYISSGTWSLLGIETKIANVSRNA
                     FSENYTNEWGAHNTVRFLKNIMGMWLIQEVARMQEYEYSYAELAELAAKEKPLQQVME
                     INDPRFLNPPNMIEEIQQYCFETEQPVPVSSGAIARCVYDNLALCYAQELIKVEHLTG
                     TKLEKLHIVGGGSNNRLLNQLTADYAGIIVEAGPSEATAIGNVAMQMLSTGQFSNIEQ
                     ARNVIKQSFPCETFYPDENKQNTMEWYVNKIKGVIV"
     gene            18158..19027
                     /locus_tag="IV334_000408"
     CDS             18158..19027
                     /locus_tag="IV334_000408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HAP8942506.1"
                     /translation="MDYWEKANHSWSEDSKRVILTPTQRSRNLFFYIQEIGYFKAPIP
                     YYTERAHLPSYLIKFTLSGKGSLTYNDNQQQLKAGDLFFIDCQEYQRYQTISEEPWEM
                     DWIHLNGQNVQSFYQEFTKDGHNTFHSTGDPSENKIHHLITELIHMQQGTNARTDFQS
                     SVAIHELLNELILQKYQLDFEEEDIPAYVYKLKQYLDEHFKETITLDQLEQQFLLNKY
                     QLSKDFSKFIGSPPIDYLISKKLSYAKDLLRYTEFTVRMISEEIGIENFAYFSRLFKA
                     KTGLSPRDFRKFS"
     gene            complement(19153..20052)
                     /locus_tag="IV334_000409"
     CDS             complement(19153..20052)
                     /locus_tag="IV334_000409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HAP8942507.1"
                     /translation="MLQKKISGEQVNTMVSDQYEIYHVKDVVSQNKTIYHHHDFYEIH
                     ATLKGEATFYLNGRQFTIKAGSVLLIHSNDLHRIVRQSSDVFERVYIFVTPSFLESRS
                     TPWSNLSACFQPMGEKRSRVLQIEQEVLKQKLSFIDHPPDKKIYGEDIKYEQALVEYL
                     IFLNQVVQKEESAQEQNAVVPNERVEQMLQYISKNLSHPLSLEEMEKQFFVSKYHITR
                     EFKKYTGFTFHQYVMKKKLIYAKHLLREYRSSSAVYSKSGFSSYPHFLKAFKREFGIT
                     PKEFLKRDTQKQLVHYEHFEDEQ"
     gene            complement(20217..21041)
                     /locus_tag="IV334_000410"
     CDS             complement(20217..21041)
                     /locus_tag="IV334_000410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HAP8942508.1"
                     /translation="MLNITIRGHDLSEVQTIEDLAEKTKEQGIHTLQLALGLSFPEIP
                     SGANEINSGMGNYVKRVLEKQEVTVGILSCYINMIHPDLTIRQELLTKFEQYLRYASS
                     FGASMVASETGCVLPEIQYTEENFTDEAFAEAVSVIRRLVKAGEKYQMMVGIEPGLNH
                     PVYSLARVEQLIQAVDSDYLGIILDPTNLITSSDYQEQVQLVEEAFERFGEKICAVHL
                     KDFRVEQEKIVPVNLGDGVIEYTKIKEIIMKNRPYLYVVLEETKDDGIRYGRSLLE"
     gene            complement(21052..22347)
                     /locus_tag="IV334_000411"
     CDS             complement(21052..22347)
                     /locus_tag="IV334_000411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HAP8942509.1"
                     /translation="MAIEAGLVLFFVFVVLLVLGMPIAISIAFSSMMTLLLVIPFDVS
                     AFSSAQKMVGSINSFSLIAIPFFVLSGIIMNNGGIAKKLVDFAMLFVGRVPGALAHTN
                     VLGNALFGSISSSAIAASTAIGGVLIPQQVEEGYDRKFATAVNIASAPTGMVIPPSTA
                     FIMYSLVAGGASISALFMGGYLVGALWALGIMLVAFIVAKKNKYPVVEKGQMAHAGKI
                     IKEAIPSLLLIVIIIGGILTGIFTAVEASAIAVIYSLLIAMFYYKTVKLKDIPNMLFQ
                     SVAMSGTIMFLLATSSMMSFAMAFTGIPQAISSLIMGVTDNKFIILLLVNLVLLLVGM
                     FMDVAPAILIFTPIFLPIVTSVGVDPVHYGLFSIMNLCVGSITPPVGTGLYVGASVGG
                     VKAEAMLKPLLPFYGVILVVLFLITYFPQIVMWLPNMLS"
     gene            complement(22363..22842)
                     /locus_tag="IV334_000412"
     CDS             complement(22363..22842)
                     /locus_tag="IV334_000412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HAP8942510.1"
                     /translation="MKMILNKVLEILGAVLLVGMVAVVLWQVFSRTVLGNPNTVTEEL
                     VRFGLVWFAMLSSAYVVGQKAHLAVTVLSEKLTGKKSKILEVVVQSLFLVFAAMIMVY
                     GGWNAVTLTMGQISPSLSIPMGYVYLSVPVSGVLIIIYSLINLIDTLSNKPAIQEKI"
     gene            complement(22848..23876)
                     /locus_tag="IV334_000413"
     CDS             complement(22848..23876)
                     /locus_tag="IV334_000413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HAP8942511.1"
                     /translation="MKKTSLLFAASSLSLGLLGLSGCGNNSSVATVADADVNEIVTLR
                     FAYASNSQPVIDAMNEFGRLVKEKTDGSVQIQYFPDGQLGGERELIELTQSGAVDFTK
                     VSASALESFSKDYSIFSVPYLFNDEEHFFKVMDNEEIMEPIYQSTKNLGFTGLTYYDS
                     GQRSFYMVDGPIHTPEDLKGKKIRVMQSETAIKMVELLGGSPVPMGSDEVYTSLQSNL
                     INGSENNEFVLYTAGHGGVAKYYSYDEHTRVPDIIIMNDAIKEQLTDEQEKAIEEAAK
                     ESTVFEIEAFAKAIEEEKALAQEEHGVQFNDVDNTPFREAVAPLHDIFKNDPTYKDLY
                     QKIQEQGA"
     gene            complement(23893..24999)
                     /locus_tag="IV334_000414"
     CDS             complement(23893..24999)
                     /locus_tag="IV334_000414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HAP8942512.1"
                     /translation="MIIKEEIFVPKELVKEKIDLLVENLTTIKDDNGEFLLDFDGLKV
                     DDKSWTVWNWPQGVGLYGIYKNYQMTKNPRAYQVVNEWFEDRMEEGAPPKNVNTMAPL
                     LTMAYLYEDTKDSRYLPYLEQWAEWVMNDMPRTNEDGLQHATYGPENKNQLWDDTLMM
                     TVLPLAKIGKLLNRPEYLEEARYQFMIHIKYLMDKRTGLWYHGWTFEGNHNYAEAFWA
                     RGNCWLTIAIPEIIEILELSKEDALRKLLIETLEAQVRALKTYQSESGLWHTLLDDPS
                     SYVESSATAGFAYGILKAVHKRYLPQEYKEVAYRAIQGLLEQIDEKGEVQNVSIGTGM
                     GDSLDFYRNIGITAMPYGQSLTVLCLTELLVSYC"
     gene            complement(25228..27825)
                     /gene="adhE"
                     /locus_tag="IV334_000415"
                     /gene_synonym="adhC"
     CDS             complement(25228..27825)
                     /gene="adhE"
                     /locus_tag="IV334_000415"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HAP8942513.1"
                     /translation="MAKVIEKEKTKTIDVQAMIDDLAEKANAALKEMESFDQEKVDHI
                     VHEMAMAALDQHMPLAKMAVEETGRGVYEDKAIKNMYASEYIWHNIKHDKTVGVINED
                     VQKGLIEIAEPVGVVCGVTPTTNPTSTTIFKSMIALKTRNPIVFAFHPSAQKSSAEAA
                     RVVRDAAIAAGAPENCIQWIEHPSIEATSMLMNHPGIAIVLATGGAGMVKSAYSTGKP
                     ALGVGPGNVPAYIEKTAKIKRAVNDLIVSKTFDNGMICASEQAVIVDKEIYAAVKAEF
                     QAHQVYIVKPDELQKLEDAVMNEGKYAVNPSIVGHSAMEIAKLAGISVPKGTKMLIAE
                     LEGVGPDYPLSREKLSPVLAMIKANNTDHAFDLCEGMLNLGGLGHTAVIHSENEELHV
                     KFGLRMKACRILVNTPSAEGGIGDIYNEMIPSLTLGCGSYGKNSVSRNVSAVNLINVK
                     TVAKRRNNMQWFKLPPKIFFEKNSLLYLEKMENVERVMIVCDPGMVQFGYCDTVREVL
                     ARRKNDVKIEVFSDVEPNPSTNTVYAGTKLMADFKPDTVIALGGGSAMDAAKGMWMFY
                     EHPDTSFFGAKQKFLDIRKRTYKIDKPEKTQFVCIPTTSGTGSEVTPFAVITDSETHV
                     KYPLADYALTPDVAIVDPQFVMSVPASVTADTGMDVLTHAIESYVSVMASDYTRGLSL
                     QAIKLVFDYLEKSVKTPDMESREKMHNASTMAGMAFANAFLGICHSVAHKIGGEYGIP
                     HGRTNAILLPHIIRYNAKDPQKHAMFPKYDYFRADTDYADIAKFLGLKGETTEELVEA
                     LATAVYELGKSVGINMSLKAQGVTQETLDTTVDRMAELAYEDQCTTANPKEPLISELK
                     QIIIDAYNG"
     gene            complement(28130..28510)
                     /gene="yajC"
                     /locus_tag="IV334_000416"
     CDS             complement(28130..28510)
                     /gene="yajC"
                     /locus_tag="IV334_000416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HAP8942514.1"
                     /translation="MGTLPMLVMFIALLAMWFFMSRSQKKQQQERQNLLDSMKPGDSV
                     VTIGGLHGVVSEIDNDKRTVVLDCEGIFLEYDRAAIKTVKPGTVVTNDATTTVETTEE
                     PAETVEVKTETTETLEETEEKEEK"
     gene            complement(28607..29752)
                     /gene="tgt"
                     /locus_tag="IV334_000417"
     CDS             complement(28607..29752)
                     /gene="tgt"
                     /locus_tag="IV334_000417"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010769586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HAP8942515.1"
                     /translation="MTQPAIRYRLIKKEKHTGARLGELITPHGTFPTPMFMPVGTLAT
                     VKTMSPEELKEMGAGVILSNTYHLWLRPGEDLVEEAGGLHKFMNWDQPILTDSGGFQV
                     FSLSDMRNIEEEGVHFKNHLNGSRMFLSPEKAINIQNKLGSDIMMSFDECPPFDESYD
                     YVKKSVERTSRWAERGLKAHANPDRQGLFGIIQGAGFEDLRRQSAKDLVGMDFPGYSI
                     GGLSVGEPKSEMNRVLEFTTPLIPENKPRYLMGVGAADSLIDGVIRGVDMFDCVLPTR
                     IARNGTCMTSQGRLVVKNAKYAHDFRPIDEKCDCYTCKNYTRAYIRHLIKCDETFGIR
                     LTSYHNLYFLLNLMKQVRQAIMDDNLLEFREAFFEEYGFNKENAKSF"
     gene            29970..30668
                     /locus_tag="IV334_000418"
     CDS             29970..30668
                     /locus_tag="IV334_000418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HAP8942516.1"
                     /translation="MQSSAELKRQAKESLKGRWGQAVLLNLIPTLIGIATILLIMIPI
                     ILFATTIYDGSASVSEATTSGGGSSGGGGGIISSIISALFMSGISWTYLDILRGQKDQ
                     IEPFKDAFRGFKGLFIGGVISLAVLISIFTTLWGLLLVIPGIIKSYSYSQSYFIYYDI
                     VQETGQTPKVLDTITASRKLMDGYKGQLFWLDLSFIGWHILALATAGIGYLWLSPYIS
                     ATKAAFYEQLPKDI"
     gene            complement(30836..33475)
                     /locus_tag="IV334_000419"
     CDS             complement(30836..33475)
                     /locus_tag="IV334_000419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HAP8942517.1"
                     /translation="MEEYKQTIEEVKKVTEADPDTGLSSQEVDERRKKQGLNKFDEAP
                     KESMIKKFFRSLSDFTTIILLVAAVISFYTAFATEHGDLFEGLLIIAIVVINSVLAIV
                     QEGNAEKALESLQDMNKQTATVIRDGKVTTVESEQLVVGDILVLESGDAISADARLIE
                     ASQLRVEESALTGESEAVEKDAAFIAEEDESLGDQLNMVFKGCTVAAGRGKAIVTAIG
                     MATEMGKIADLLNENTMQKTPLQVRLNQLGKRISMIALAAAALVFVIGELQGEPLLEM
                     FMTSISLAVAAVPETLTVIVTLTLAYGVQKMARKHAIIRQLPAVETLGTANVICSDKT
                     GTLTQNEMRVRRVWSKEDEVTNIEDSMTNSAMEILKMAALCTDVTVEQEDDDLVIKGN
                     PTEVAIVRAVEENYHTKAELEEKYPRVNELPFDSERKMMTTVHQMGEKYISITKGAFD
                     VLAPRFSSGDVEQAAIVNDRFGKRALRVIAVGYATYDEEPQDISSEALEKDLRLIGLI
                     GMIDPPRPESKGAIKRAKKAGIKTVMITGDHVVTASAIAKELGILNDPSEALSGSELH
                     QLSDEELDARVKALSVYARVTPEDKIRIVKSWQRTGAVVAMTGDGVNDAPALKASNVG
                     CAMGITGTDVAQSAADMILTDDNFATIVDAVSQGRSVYQNIRKAINFLLSCNISEIFI
                     VLIAMLLGWGAPFTAVQLLFVNVVADGLPGFALGREPAEHGIMDQPPIPKNEGIFARG
                     LLQKIAINAGVFTIVTLFGYYLGSYVDTISPWVDASQHVGQTVAFLILAYSSILHVFN
                     VRSSQSIFKVNLATNKALVEMALLALAITTAVALLPFTQELFGLVHISLNHWFLVGIL
                     SIVPIAVNELIKFHRLPEAEEEE"
     gene            complement(34094..35125)
                     /gene="queA"
                     /locus_tag="IV334_000420"
     CDS             complement(34094..35125)
                     /gene="queA"
                     /locus_tag="IV334_000420"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HAP8942518.1"
                     /translation="MLTTEDFDFDLPEELIAQTPLKDRDHSRLLVLDRKTGEMEDKHF
                     HEIIEELQPGDALVMNNTRVLPARLYGEKPDTGAHLEVLLLTNTQGDTWETLIKPAKR
                     AKVGTRISFGDGRLQAVVKEELEHGGRIIDFEYEGIFLEILESLGEMPLPPYIKERLE
                     DPDRYQTVYAKENGSAAAPTAGLHFTKELLDEIEAKGVELVYLTLHVGLGTFRPVSVD
                     KIEEHHMHSEFYRLTEEAAERLNQVRKNGGKIVAVGTTSIRTLETIGTKFNGEIKADS
                     GWTDIFITPGYEFKVVEAFSTNFHLPKSTLVMLVSAFAGRELTLSAYQHAIEERYRFF
                     SFGDAMFVK"
     gene            35318..36493
                     /locus_tag="IV334_000421"
     CDS             35318..36493
                     /locus_tag="IV334_000421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628506.1"
                     /note="Members of the family are the ATP-binding subunit
                     of ABC transporters for substrates such as betaine,
                     L-proline or other amino acids, choline, carnitine, etc.
                     The substrate specificity is best determined from the
                     substrate-binding subunit, rather than this subunit, as it
                     interacts with the permease subunit and not with substrate
                     directly; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="betaine/proline/choline family ABC transporter
                     ATP-binding protein"
                     /protein_id="HAP8942519.1"
                     /translation="MIEFQNVSKIYKGGKVAVEDVNLSFEKGEFICFIGTSGSGKTTT
                     MRMINRMTDPTQGKILIDGKSIQEIDPVELRRQIGYVIQSIGLMPHMTIRENITLVQK
                     LLKVSQEERNKTAEKMIDLVELPREMLDRYPHELSGGQQQRIGVVRALAANQDIILMD
                     EPFGALDPITRDSLQDLVKDLQERLGKTIVFVTHDMDEAIKLASRIAIMSEGRVIQFD
                     TPENILRHPANDFVEELIGEDRLLQAKPNATRVGEVMLNTAITITPEKSLQEAIKLMR
                     EKRVDTLLVTDNSNVLKGFIDVETLNKKRGKVSSVGDILNKHVFYVKESAYLRDTLQR
                     ILKRGLKYVPVVDEQNKVVGILTRASLVDIVYDVIWGEDPATTDVAESATPEESKEV"
     gene            36497..37138
                     /locus_tag="IV334_000422"
     CDS             36497..37138
                     /locus_tag="IV334_000422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP8942520.1"
                     /translation="MQQFFHQHGSEILSKGFEHLYISGLALIIGIIIAVPLGVLLTRF
                     PKAATIVIGLTSALQTIPSLALLALMIPLFGVGKLPAIIALFIYSLLPILRNTYIGMK
                     NVDWNYRDVAKGMGMTEFQSIFSVELPIAMPTIMAGIRLAAVYVIAWATLASYIGAGG
                     LGDLIFSGLNNYQPDLILAGTIPVTILALLADWLLGLLEHRLTPIALREENEE"
     gene            37135..38052
                     /locus_tag="IV334_000423"
     CDS             37135..38052
                     /locus_tag="IV334_000423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmoprotectant ABC transporter substrate-binding
                     protein"
                     /protein_id="HAP8942521.1"
                     /translation="MKRILKMTMVLFSLLILASCSFPGLASNTDEDTISITGGITSEA
                     QILASLVAGMVEHYTDKNTAIINNLATTTINHQAMMNGDASISAARYTGTDLTTTLNL
                     PPEKDPKKAFATVKDEFEKRYGQTWFPSYSFENTYVFLVRKDTAQKYHLSKVSDLKNV
                     ADELVAGVDTSWINRKGDGYDGFQETYGFSFNSILPMQIGLVYDAVEAGKMDIVLGYS
                     TDGRIASYDLVMLEDDRQFFPPYDAAPVVDNKILADTPHLKEALNKLGHTISTEKMQE
                     LNYEADNNLVEPSVVAQRFLQENHYFEGK"
     gene            38049..38720
                     /locus_tag="IV334_000424"
     CDS             38049..38720
                     /locus_tag="IV334_000424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP8942522.1"
                     /translation="MKQLDKMNVFQQFFYYFQENGSYIFAQFIRHFLISIYGVLFAAV
                     VGIPVGIMISRRRKLANWVIRLANIIQTIPSLAMISILIIGLGLGVNVVIVTVFLYSL
                     LPIIKNTYTGMIQVDKNILDVGKGMGMTARQRLFMVELPLSVSVIMAGIRNALVVAIG
                     ITAIGAFVGAGGLGDIIIRGTNATEGTSIILAGALPTALMAIITDWLLGILERRLDPA
                     SRTSR"
     gene            complement(39261..39749)
                     /locus_tag="IV334_000425"
     CDS             complement(39261..39749)
                     /locus_tag="IV334_000425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-glycoprotein
                     endo-beta-N-acetylglucosamidase"
                     /protein_id="HAP8942523.1"
                     /translation="MTRKISKIGASLLVLAVATTIDYRQSEAKTIGNVVYRETAKSML
                     KKAMDNQPESSYWFPEDSLAWSFGKDTDTKYNTSVVPLAERVSKATLPNMNVTQTEEV
                     KVVALSAMNSSINGNASRGIDIFYANVFSYWQYIDQLVYGGALLVKDHRIAKSSCDGC
                     SP"
     gene            complement(39950..40216)
                     /locus_tag="IV334_000426"
     CDS             complement(39950..40216)
                     /locus_tag="IV334_000426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HAP8942524.1"
                     /translation="MSNYTVAIKNSAKVDLRKIKQTNLKKQFEEVIQTLKEDPYMPTQ
                     SFEKLRPTHEGRYSRRLNRQHRVVYKVDEENKVVEIYSAWTHYE"
     gene            complement(40216..40479)
                     /locus_tag="IV334_000427"
     CDS             complement(40216..40479)
                     /locus_tag="IV334_000427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HAP8942525.1"
                     /translation="MANTTMNPSTARKNFYQLLKEVNENHTEIEIISDRSGNNAVLIG
                     LEDWRAIQETLFLEQTGTLDKVRDREKDDSSFTNIDDIDWEAL"
     gene            complement(40472..40744)
                     /locus_tag="IV334_000428"
     CDS             complement(40472..40744)
                     /locus_tag="IV334_000428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8942526.1"
                     /translation="MYWCCCIDSQALDSTFAKDDTLLPITLRLATASRKGVRSERGQR
                     SSLKRGLFLYVKKRKSLLNLSALLYFFDVLNQVRYNYIRKRRRDNG"
     gene            40955..41140
                     /locus_tag="IV334_000429"
     CDS             40955..41140
                     /locus_tag="IV334_000429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAP8942527.1"
                     /translation="MALSYGNKKWSLDEAEIPTYEDTWHLRVEVKEEAENIGGCTLFG
                     KGFGNYDQDIKIKMFYS"
     gene            complement(41282..43105)
                     /locus_tag="IV334_000430"
     CDS             complement(41282..43105)
                     /locus_tag="IV334_000430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HAP8942528.1"
                     /translation="MDYLKRLLVGKPLKSAENDEHKLTRFAALALLSSDALSSIAYGT
                     EQIVVVLVALSAAAIWYSLPIAAFVIILLISLTLSYRQIIHAYPHGGGAYVVSSENLG
                     KNAGLISGGSLLIDYMLTVAVSVSAGAEAITSAVPALYGHQVAISVTIVLLLMMLNLR
                     GLRESASFLLFPVYTFILVISLLIVVGLYNIVTGAVPLQATALPGAAVPGVSIALILR
                     AFSSGSSSLTGVEAISNAVPFFKKPRAKNAAATLTMMALILGFFFVGITFINYWYGIV
                     PEKEVTVLSQIGKAVFGHGILYYVLQFATALILAVAANTGFSAFPVLAYNLAKDKFMP
                     HMYQDRGDRLGYSNGIITLALGSIVLLFIFHGSTERLIPLYSIGVFIPFALSQTGMVV
                     KWKKEGKRWLSKSIANITGAFISYAIIAILFVYRLGDIWPFFIIMPIVMFIFYKIHDH
                     YKKVAEQLRLENDAKLHDYDGNTVLVLVGNVTRVNIGALNYARSIGDYVVAMHVSLDE
                     DVEKEKEIEAEFKKHFPDVRFSIVHSSYRSIENPIIRYVDIVSKNAAKQNYTTTVLIP
                     QFVPNRRWQNILHNQTSLRLRLRLSWRENIVVSTYSYHLKK"
     gene            complement(43703..46408)
                     /locus_tag="IV334_000431"
     CDS             complement(43703..46408)
                     /locus_tag="IV334_000431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhgE/Pip domain-containing protein"
                     /protein_id="HAP8942529.1"
                     /translation="MQSIKNTLRLYLLDWKRIFKNPIATLLIIAIMIIPSLYAWFNIK
                     ALWDPYGNTGELPIAVYSADKPAEFQGKEVAIGEQVIESLHKNKQLGWQFVDSKEQLE
                     DGVRSGKYYAGIYLPKDFSEDLLSFTSGDIKKPKIEYTVNEKINAIAPKITDKGASSI
                     QSQITNEFIKTASSTLLKVFNEIGYDIDTNLVSINKVKDMILSTDENLDTIDGYTKQV
                     LELQSQLPEIKEKLNKANEFVDYIPKVDEMGEKVVALNDKMPELKDQAKIILDLQEKI
                     PEIQNAGKQLAEIDGDFASIQETMNQGIDEAKQGLTIIQQVQDILPDVKNLENQASDL
                     ANQTKSGASQLKDAIPSITNSVDVTLKSIGQVATTTGSLIDTVRTAIEDGKLTDDEKK
                     NISNVLNGFIDGISQQQNAIDKLVEFLTKLQTSAGNTKLQPIIDQLNQLKPLLSNLQN
                     RLSALNTAVQNEDTKAIDELLDQIDAVVNNINGIIAGINTGQISSTVTSLLDQVIDTI
                     GTAQGALSKADQIDFDSLLSSTKATVTNAVAILEKYQKEMPAIGQEIHDANTMLNGNM
                     DTIVNGINEGADLYNNELPIIQQKLGLAADFIQNDWPALKNEITGTLTTVNEKMPEVE
                     TAVNAAADLINNDWPNIKAGIQKAADAIRKGEQEVDLGEVIKLLKLDATKESDFFTQP
                     VELQTNALYPIANNGSASTPFYTALCLWVGAVLLSSVATTEYYLGKKDRNNFTKREQF
                     VARMLTFLTMAIAQSLIVTLGNIFLLGVDVQNPVYSVSFAVLIALAFMMIVYVLAALF
                     DNVGKGIAIIILVLSISGGGGNYPIQVSGKFFQMINPFLPFTHAVNLLRESAGGIYWP
                     NATKAIWIMIGLFVVFGIVGTAIYPFIEEKMTKFKEAAHESHIFH"
     gene            complement(46714..47661)
                     /locus_tag="IV334_000432"
     CDS             complement(46714..47661)
                     /locus_tag="IV334_000432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxidoreductase"
                     /protein_id="HAP8942530.1"
                     /translation="MRRFAINGYGEAQDVFEEIDAHPREVGSGHVRVEIKAFSVNPYD
                     VALRLGEMKEIRTLKFPYVPGNDGAGIVTEIGSDVTTVHVGDRVAVHAVGGTYGEEVV
                     LPSAKVAKIPDKMSWEEAAGMVTPGITAYNLINHLTEIQPTDTVMILGASGAVGSSLI
                     QLLHEKGIRILTSASSKNEEKVKKLGASAFAAYDKTNPGLQFADQADLVIDATKGSIK
                     GETGIQIMKPGGRYVALNDLPDLDLRQKKEGFYESFVPRKEYLDAEAFAGIIKAYQKG
                     AFHVFISMNLSASLKHVIQAHQLVEGHPPAGKIILSFEK"
     gene            47851..48120
                     /gene="rpsN"
                     /locus_tag="IV334_000433"
     CDS             47851..48120
                     /gene="rpsN"
                     /locus_tag="IV334_000433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HAP8942531.1"
                     /translation="MAKKSKIAKAKKQQETIERYAALRQELKAQKDYEGLRKLPLDAR
                     PERLKNRDLIDGRPRGYMRKFGMSRIKFRELAHKGLIPGVKKASW"
     gene            complement(48296..48658)
                     /locus_tag="IV334_000434"
     CDS             complement(48296..48658)
                     /locus_tag="IV334_000434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HAP8942532.1"
                     /translation="MVKRGDIYFADLSPVVGSEQGGTRPVLVIQNNLGNHFSPTIIVA
                     AITAKMAKPKLPTHIGIKATGTGIERDSVILLEQIRTIDKSRLKEKVCHLDRPIMAEV
                     DRALKISVGIEALTPVTK"
     gene            complement(48669..49790)
                     /locus_tag="IV334_000435"
     CDS             complement(48669..49790)
                     /locus_tag="IV334_000435"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HAP8942533.1"
                     /translation="MVVAWHRPTKAVIHKKAITENVANEVARLPQGKELFAVVKANGY
                     GHGAIETAEAAVAGGASGFCVSNLDEGVELREAGFTQPILILNMVPYDALTVAVAHDL
                     SVTAGTREWLQAAAAVLEKSKLETPLSIHLKADTGMGRIGFCTPEEVKEAAAFIKESR
                     VLEWEGLFTHFSTADQADDTYWNLQKERFIEVLKKLPELPRYVHVSNSATALWHDETI
                     GNMIRYGVAMYGLNPSGHALPEVYPLQPALELVSELIQVKKLPAGEGIGYGETYITPE
                     AEWIGTIPIGYADGWPRKMQGFSLLVEGNYCETIGRVCMDQLMIRLPQEFPVGTKVTL
                     IGKNADKEITMQDIADQLGTIHYEVACGLGQRIPREYQE"
     gene            complement(49806..50156)
                     /locus_tag="IV334_000436"
     CDS             complement(49806..50156)
                     /locus_tag="IV334_000436"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HAP8942534.1"
                     /translation="MIKGIGIDAVELPRITRLIEEKPKFIARILTSDEMKLFQSLPFH
                     RQVEFLGGRYACKEAFSKAWGTGIGKVTFQDVEILKNENGQPVVTRSPHEGNVWVSIT
                     HTNETAFAQIILED"
     gene            complement(50286..51797)
                     /locus_tag="IV334_000437"
     CDS             complement(50286..51797)
                     /locus_tag="IV334_000437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HAP8942535.1"
                     /translation="MKFKELELSPELLKSVERAGFEEATPIQAETIPLALAGKDVIGQ
                     AQTGTGKTAAFGLPMLEKIDPDRHELQGLVIAPTRELAIQTQEELYRLGRDKKVRVQA
                     VYGGADIGRQIRGLKDRPHIVVGTPGRMLDHINRHTLKLGTVQTLVLDEADEMLNMGF
                     LEDIEKIISQVPDQRQTLLFSATMPPAIKNIGVKFMKNPHHVKIKAKEMTADLIDQYY
                     VRAKEYEKFDIMTRLFDVQTPELTIVFGRTKRRVDELARGLEARGYRAEGIHGDLSQQ
                     KRMSVLRSFKSGHLDILVATDVAARGLDISGVTHVYNYDIPQDPESYVHRIGRTGRAG
                     KGGMSVTFVTPNEMDYLHVIENLTKKRMTTLRPPTEKEAFKGQLGAAVEQIETKLAEN
                     GLDKYLQTADKLLEEYSAQDLVALLLKTTAKDPADAVPVKITPERPLPMQKKGYNKNG
                     KRGGGNNRNRRDGGNYRGNKSKGGYSKNNNSQKDGGKRHNDKKRGFVIRSNND"
     gene            complement(52119..53471)
                     /locus_tag="IV334_000438"
     CDS             complement(52119..53471)
                     /locus_tag="IV334_000438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HAP8942536.1"
                     /translation="MNLTIQEIAQAVGSECSALTKITNVEFDSRKITEGSLFVPLAGT
                     RDGHEFIQQAITNGASASFWSLPVEQAPKNFPVIPVADPLKAMQQLATYYLEKVGAEV
                     VAITGSNGKTTTKDLTASVLSEKYKTYKTQGNYNNNIGLPYTILHMPQDTEKIVLEMG
                     MDHANEITELSLMAQPKAAAITMIGEAHIENLGSREGIAKAKMEITDGLVSDGLLVVP
                     ADEPLLTPLTATLVQTVTTFGIGNGDVHAEVTAKGKAQTDFVVEGETFTIPLPGSYNV
                     TNALIAYTIGRFFGLSIPEIRQGLAHVSITQNRTEWLTAGNGAAILSDVYNANPTAMG
                     LVLDTFKNLPTKGRRIAVLADMLELGKESLNMHAQMSEHIDPEKFAMIFLYGEQMHAL
                     KEELNQKYPQLPVFYFEKEKNKLIDAIKQNLEATDSIMLKGSNSMGLFEVVEQLQQMK
                     "
     gene            complement(53624..54700)
                     /locus_tag="IV334_000439"
     CDS             complement(53624..54700)
                     /locus_tag="IV334_000439"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HAP8942537.1"
                     /translation="MKITLLYGGRSAEHEVSILSAFSVLNAIYYNYYQVQLVFITKEG
                     QWVKGPLLTEKPASKDVLHLSWDPSGQTEEGFTGKVINPGEIKEEGAIVFPVLHGPNG
                     EDGTIQGFLETLNMPYVGAGVLTSACAMDKIMTKYILQAAGVPQVPYVPVLKNQWKEN
                     PKKVFDQCEGSLLYPMFVKPANMGSSVGITKAENREELQNALATAYQYDSRAIVEQGI
                     EAREIEVAVLGNEDVRTTLPGEVVKDVAFYDYEAKYINNKIEMQIPAEVPEEVYQKAQ
                     EYAKLAYTMLGGSGLSRCDFFLTNKNELFLNELNSMPGFTEFSMYPLLWENMGLKYGD
                     LIEELIQLGMNRYHQRQSFFEKNE"
     gene            54896..56641
                     /locus_tag="IV334_000440"
     CDS             54896..56641
                     /locus_tag="IV334_000440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AarF/ABC1/UbiB kinase family protein"
                     /protein_id="HAP8942538.1"
                     /translation="MAQTNPRSRLAEIIRVFINYNVVPNFVQQKNPEQVKKAFEELGP
                     TFIKIGQMLSVREDLLSSAFTQTFKTLQDSVPSDTFSTVKKTIETELSLSLSDIFDDF
                     SKSPFASASMGQAHRAKLKNGDAVVVKIQHPNIAEEIRLDLQLFERAIPLIKYIPETS
                     VVDLKGVLQEVKRSLINEMDFLKESQNGEQFYQKNNGWKEIRSPKIYDAFCSKKVIVM
                     EEMSGKNLNHLMNAENKTETFITGIQNKQLKQEVAKLLVENFMKQVFDDGFFHADPHP
                     GNLLFHVLTKEEQTQASRKTETVHEKEFGSFAFRASTSAEDPVAPYTINYIDFGMMGH
                     LSAGLRQKLTQAVLALYTKDAYRIEKAVLRLCQQEGSFDESRFHQELTSFLEQYYDSP
                     IDEINLQEVFTHVVTICHQNNLQFDRDITLLLKAFGTLEGVIRVLDPEVSLMEVASPF
                     AQHYFLTHLDVEDTLKQSGLDLLEGMKAAPKIPQQLHHLLEMWTSGQGKVNLELKKQD
                     KLLSRIESMINRLVFGMILAALIVGSSLLVQAAPVENAEVVSLLGIFTYAIAAFVIIF
                     LAIDALIQMYKKRKK"
     gene            complement(56921..57358)
                     /locus_tag="IV334_000441"
     CDS             complement(56921..57358)
                     /locus_tag="IV334_000441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiI/YqxD family protein"
                     /protein_id="HAP8942539.1"
                     /translation="MRLFIDGDGSPVKEDVIKLGESYGLDVVIVTSVDHYTTKEYANH
                     VQIVYVDRGADSADYKIVGMVKKNDFLITQDYGLASLVLPKGVRVFHHSGKEFDEANI
                     GTLLEQRYHSGKLRKAGIRTKGPKPFTEKDHMSFRQALKKAID"
     gene            complement(57342..58715)
                     /locus_tag="IV334_000442"
     CDS             complement(57342..58715)
                     /locus_tag="IV334_000442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8942540.1"
                     /translation="MFEKLKRFGRNFTQTTPFILSNTVIFIPYLLFLGLTDSHNWLTI
                     LPFTLFYAFRMTGLFLVNSLRFGLDSYTLLMIALLIGGAGSLLGILGVFYFPLFYFSS
                     VLLGLSAAWLVPANTTVNFHEKTQGFINMDGKKYLFAAIWLFLLYQAIELPAPTQIAA
                     SFTIYTLFYVMAYHTVSHYPRYELDFKDIKRNLVATRELVLFLIFFGLLFLLRNARLL
                     FDERLFDLAIYGFLLLFILFVFVLGHQKKEWKLPSWLNLFTFLNGMLGNFLFLFSSFY
                     VGILYGLEKLSIFMYLPYAAGLIAAKIVGPWILKRLTWSPLAMQLLGLSSSLLVLLIP
                     NGFAIGVFLLSFWHVVTGSWLNKEYYQTDTAIPMDRRIVVKYTTQNKGSVIHQFLLMT
                     LLFVLAKEMGEPIHLLLITLNHESVPIESVQLLILAKWINVGLLLSGLVTVYHLWKKA
                     KKNEAIH"
     gene            complement(59037..59525)
                     /locus_tag="IV334_000443"
     CDS             complement(59037..59525)
                     /locus_tag="IV334_000443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP8942541.1"
                     /translation="MEKIKVTENELDELMAVIQEVWPEVFVPIIGQKQVDYMLKTYQS
                     KKQIQKELTEGVSYFLLKSEEKTVGYTAYEEINGKIYLSKLYLLNKVRGQGLTSSVFR
                     WYEELAAQTKEREIFLRVNQGNHQAIEVYKHEGFEITEELISDIGQGFQMVDYKMKKA
                     LA"
     gene            complement(59547..60149)
                     /locus_tag="IV334_000444"
     CDS             complement(59547..60149)
                     /locus_tag="IV334_000444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HAP8942542.1"
                     /translation="MSKKGSVLQKCLQIMPIIGLFFFFVLILYAYHHGIFRSSTSLQA
                     FIQQFGKYAVMIFVLLQVIQVIIPILPGGISSVAGMLMFGNGFGLLYSCIGLVIGEAI
                     GFLLVRYYGVAFVQLILSPKKYQKFDELLTRKTKDIKKVLVVTLLLPFAPDDLICLVA
                     GLTKLSFREFMQIVIFLKPWSVGVYSMIMLFLFHQAQGHL"
BASE COUNT        17104 a        13016 c        10989 g        19083 t
ORIGIN      
        1 tattttagac ttatacgtta ttgtcctgtt tcatttgatg gaggattgta aataaaaaaa
       61 gaggaaagaa cataactttt cgttatcttc tttcctcttt ataattgttt agcttattta
      121 gccaaagctt cttttgcttg gtcagccaat gcagtaaatg ctgctgcatc gttaacagcc
      181 aagtcagcta acattttacg gttgatgtcg atttcagcaa ctttcaaacc gtgcattaat
      241 tttgagtagc tcaagccatt catacgagct gctgcgttga tacgagcaat ccacaattta
      301 cggaagtcgc gtttcttttg acgacgatcg cgatatgcgt agttgtatga attcattact
      361 tgttcttttg ctgttttaaa taaagtgtgt tttgaaccgt agtaaccttt tgctaatttt
      421 agcactcttt tacgacgttt acgagttact gttccacctt taacacgtgc catgtataat
      481 tcctcctaat aaaaataaat cttcagattc tgttatttgt acttcttatt tcatttttgc
      541 tagttgttgg cgaatacgtt tgaagtcgcc actagaaacc atgcttgctt tacgcaattg
      601 acggcgttgt ttttttgttt tgccgtggaa acggtgactt gtaaacgcgc ggaagcgttt
      661 caatccgcct ttaccagtac gtttgaaacg ttttgctgat ccgcggtgtg ttttttgttt
      721 tggcatgact atatttcctc ctcaaaatct ttcgttatcc gactatcggc tgtccaacag
      781 ccagttagtc attcaaatta cttactgtcg tttttcggtg ccagcgtcaa gaacatgctg
      841 cgtccgtcca ttttcgcttt ttgttccact gttgcaatat ccgcagtttc ttcagctaag
      901 cgattcaaga ctttttgacc aatttcttta tgggtaatgg cacggccttt gaaacggata
      961 gaagctttca ctttatcgcc cttttccaag aacttacgtg cattacgaag ttttgtattg
     1021 aagtcattca agtcgatcgt aggacttaag cgaacttctt ttacattgat cactttttgt
     1081 tttttacgcg cttcgcggtc tttcttttgt tgttcgaaac ggtatttacc gtagtccatg
     1141 attcgcgcaa ctggtggttt cgcaccaggg gcaactaaca caagatccaa gtttgcttgt
     1201 tctgcaatat tcaatgcttc tgcttttgtc ttaactccta attgctcacc atctgaaccg
     1261 atcaatcgta actctcgtgc acgaatgccg tcgttaacca tcatatcctt tgctatggtc
     1321 attcacctcc aaatattatg agagaaagaa aagcttaccg aataaacggc aataaaaaaa
     1381 cgagttctct tacagaaccc gcgcgtactt cacctaatag aaccagtgaa aactatctag
     1441 cccagtgaca ttatcaacta aggcgagaag cgggtgcttc tgcttttatt tctcaaccat
     1501 catagtatag caaaaactaa tctttttgtc aatgtttttc atacagtttg tcttcctttt
     1561 ctgaaaatga ttagattagc agactttcta gcagtgattc ccgcttcttt gctatactaa
     1621 agaaaaacga ggtgaaccca catgaaaaaa atcgaaccta ttccaccttc tcttcctctt
     1681 cttacaaagg gagagtttta tttagaagat gaggagatct tctctggtct tctgatcaac
     1741 gggggtgacc aaagctatct cgatgccaaa aatatggtat taagagagag tcatatccaa
     1801 aaacttacga tgcaaaaaac agttttacgt cattttgaat gcagtaatgt catttttgaa
     1861 aaatgtgatt tttccaatct ggagtggatt ggtggcagtt tccaccaagt cgtgtttcat
     1921 caatgtaaat taacaggtac gaattttgcc gaaagttatc ttcgagactg tactttcact
     1981 gattgtattg cttcgatggc ttcctttagt agtacaaact tgaaagctgt ctccttcgat
     2041 cattgtcaat tagaagacag cgaattttat gaggtcactt ggaaaaacct ttttttgtct
     2101 gacaatcagt tgaccggttc gaactggttt cgaacttctc tgaagggatt agactttacg
     2161 actaatttct tcacagcaat cgctttatct caagattctt tggcgggact aatcgtcaac
     2221 caagagcaag cacttgtgat cgcccaagcc cttggtttgc agatcaaaga ctagatcaac
     2281 gaatccgccg ccacagtgca ataagcatcc aaatagataa gaccaaactg atgatcaagg
     2341 cgattcccca tccgaaaacg gaatgggtaa agggtaactg gagattcatt ccgaagaatc
     2401 ctgtcacaat agttggaacg gtcaagatca gcgaccagac cgtcaaaaat ttcatcgtgt
     2461 cattcaagtt gttattcagc aaattattat aagtgccaga gagctggtct aaaatctgcg
     2521 aagctaagtt cgtcatttca actgcttgtc gtgcttcgat cagcgcatcg tccaactctt
     2581 ctttttcctt ttctgacaac ttgcgataaa tagccaatgc cttgatttgt tctaaaagta
     2641 ctacattttg ttttgttcct gtaactaagt aaaccaaacc gatctctaaa tctgaaagtt
     2701 gcagcagatt cttgttcgtt gttttttctc ttaatttcag attaagccgc tgacgttcac
     2761 tattcacttc ctcgattaaa ggaaaaaagg aatcagaaat caaaaacaat gtacgaaaca
     2821 ataaactgta tacactcatg tctggcattc gttccaaaag cgatcgaatt agtcgaatca
     2881 cgtactcggt attcggcgtg gtaaatgtaa acaaaccgtc ttcttttaaa ataaaagcga
     2941 taggactagt ttcataatga ttatcgactt tttgctggtg cggaacattt gatacaagca
     3001 gtaacgcttc atcaaaagaa tcgtactcta cgcgcgcccg ttcattttta tctagagaat
     3061 aagtcagcat ctcgtcacta atgccatatt cttcttttaa gtttttaaca gctagcacat
     3121 cgttttgctg tacattgatc caaaaattac ggtcttgatt aaaatagtgt ttttcccgca
     3181 tactctatcc tcctgacatt tgtttccagt ataaagaaaa aagtcgctca aataaaggtt
     3241 taccctttat ttgagcaact gtaaatcaaa tggctacttt ggtttggcga atctctgcta
     3301 atttttcttc gatcattttc aagacttctt cttttgcttg aggatcttcc acaaaattat
     3361 agcggtctcc atcgatttgg atttttggac tttctttgta ttcttcatac cattgatcgt
     3421 aacgtctgtt caattctttg taataatcgt atagacttgg atcaaaggac aattgttcat
     3481 acgaacgacc acgtttttcg atacgctcca acatcgtatc aaaagataca cgaatatgaa
     3541 ctaataaatc cggatgtttt ttatgggcag catagggaag ttcttccatc atgttttcca
     3601 gcagttcatc atacacacga acttctgttt ctgttgctct tcctagatct gcatttagat
     3661 ggaaaagcag tgaatcttca taaatcgaac ggtccaatac attattgtca tctttcattg
     3721 cttgtttgat gcttgcaaaa cgtttattca aaaaataaat ttgcagcaaa aaagcatact
     3781 gttcagggtt tgcataaaat aagggcaaga cttcattatc atcaatcgat tcataaaatg
     3841 cctgactatc aaagtgttca gccatcattt ctgttaaact tgattttccg gcaccaattg
     3901 tacctgctaa aacgatcacg ctcatttacc tccagctcac ttcatcacta aatattgtgt
     3961 ttaactccaa cctttttaaa tataccccca atatctagta gtgtcaattg tatttttttc
     4021 tagtcaaaga agaaaaaaag tcaaaaaatt cttaagaaaa aagtgttttt tctttagaaa
     4081 acgtattctt agttttactt ctgagatggt tttagtaaaa taaaggagga atgttttttg
     4141 agacccttag tggacaaaaa aattcgtgaa gcagcgcaac tcgttttggg cgctttattt
     4201 tttgcttaaa aatcactttt acgtgagaag gagttagatt atggcaaact atggccaagg
     4261 agaatcaagc ggaaagatca tattgatggg cgagcacgcg gttgtttatg gagaaccagc
     4321 gattgccttt cctttctatg caacaaaagt caccgcattc cttgaagagc tggatgcaat
     4381 ggacgatcaa ctggtttctt cctactattc aggaaattta gccgaagctc ctcatgcatt
     4441 aaaaaatatc aaaaaattat tcattcactt aaaaaaacag catgacatcc aaaaaaactt
     4501 gcaactgacc attgaaagca cgattcctgc tgaacgtgga atgggatcaa gcgctgcagt
     4561 cgccacagca gtcactcgtg ctttttatga ttacttagca tttcctttgt ctcgtgaaat
     4621 actattagaa aatgtccagc tttcggaaaa aatcgcccac ggtaatccta gtggaatcga
     4681 tgcagccgct actagcagct tgcagccgat ttattttaca aaagggcatc ctttcgacta
     4741 cttttctttg aacatcgatg cttttttgat tgtcgctgat acaggaatca aaggacaaac
     4801 aagagaagcc gtcaaagatg tcgctcacct ctttgaaacc cagcctcatg aaactggaca
     4861 aatgatccaa aaattaggat acttgacgaa gcaagcaaaa caagcaatca ttgaaaattc
     4921 accagaaacg ttggcacaga caatggatga atcacaatca cttctggaaa agctgacaat
     4981 cagcaatgat tttcttaatc tattgatcca aacagcaaaa gataccgggg cattgggtgc
     5041 caaattaact ggcggtggac gcggtggctg tatgattgca ttagcacaaa caaaaacaaa
     5101 agcccaagaa atcagtcaag cacttgaaga tgcgggtgct gctgaaactt ggatacaagg
     5161 attaggagta catacctatg tttaaaggca aagcacgcgc atataccaac attgctctaa
     5221 tcaaatattg gggtaagaaa aatgaagaac tcatccttcc aatgaacaac agcctttcgt
     5281 taacattgga tgcattttat acagagacag aagtcatctt ttctgatagc tatatggtag
     5341 atgaatttta tctagatggc accttgcaag acgaaaaagc aacaaaaaaa gtcagtcagt
     5401 ttcttgacct ttttcgtaaa gaagccggcc tctcactaaa agcttcagta atcagtcaga
     5461 atttcgttcc taccgcagct ggattagcct cctctgccag cgggctagct gctttagcag
     5521 gcgcttgcaa tactgctctt aagcttggat tagatgatct ctctctttca agatttgctc
     5581 gacgcgggtc tggttcagct tgccgaagta ttttcggtgg tttcgtcgaa tgggaaaaag
     5641 gccatgacga cttaagttct tacgctaagc cagtcccttc cgattctttc gaagacgatt
     5701 tagcaatggt tttcgttttg atcaacgacc agaaaaaaga agtgtccagc agaaatggga
     5761 tgcgtcggac agtcgaaaca tccagttttt atcaaggctg gttagattcc gttgaagggg
     5821 atctatatca attgaaacaa gcaatcaaaa caaaagattt ccaacttctc ggagaaacga
     5881 tggaaagaaa cggactaaaa atgcatggaa cgactttagc tgcccagcct ccattcactt
     5941 actggtctcc tgacagctta aaagcgatgg atgcggtgcg acaattgaga aagcaaggca
     6001 ttccatgcta ctttacgatg gatgctggcc ctaatgtcaa agttttagtg gaaaacagcc
     6061 atttatctga ggtacaagaa acatttacca aattatttag caaagaacaa gtaattactg
     6121 cacatgctgg tccaggaatt gcgattattg aataaaggga gatttttatg attgaagtat
     6181 ctgcaccagg caaactctat attgccggag aatatgcagt tgttgaaaca ggccatccag
     6241 cagttatcgc tgcagtcgat caattcgtga cagtcactgt agaatctgca cgaaaagtcg
     6301 gaagtatcca atctgcacaa tatagtggga tgcctgtacg ttggacaaga cgcaacggag
     6361 aattagtttt agatattcgg gaaaatcctt ttcattatat ccttgctgct attcgcttga
     6421 ctgaaaagta tgcacaagaa aaaaacattc ttttatcctt ttatgatctg aaagtaacaa
     6481 gtgagttaga cagttcaaat ggccggaaat atggattggg ttctagcggt gccgtgaccg
     6541 ttgcaacggt caaagccttg aatgtttttt acgcgttgaa tttatctcag ttggagattt
     6601 tcaagattgc agcactagcc aatttagcag ttcaagataa tggttcctgc ggcgacatcg
     6661 ctgctagctg ttatggtggc tggatcgctt tctcaacctt cgaccatcct tggctccaag
     6721 aacaagaaac tcagcattct atcagtgagt tacttgccct ggattggcca ggtctatcca
     6781 ttgagccatt gattgctcct gaagatttac gtttattgat tggttggacg ggtagccctg
     6841 cctctacttc tgatttggtc gatcaagttc accgttcgag agaagataaa atggtggctt
     6901 atcagctttt cttaaaaaat agtacagaat gtgtcaacga aatgatcaaa gggtttaaag
     6961 aaaataatgt aacgttgatc caacagatga ttcgaaaaaa ccgacaatta ctgcatgatt
     7021 tatctgcaat cactggggtc gtcatcgaaa cgcctgcttt gaacaaattg tgtaatttag
     7081 ctgaacagta tgaaggagcc gcaaaatctt ctggtgcagg tgggggcgat tgcggaatcg
     7141 taattgttga ccagaaatct ggcattcttc ctttaatgag tgcatgggaa aaagcagaaa
     7201 tcactccact gccgttacat gtctatagcg atcaaagaaa ggaaaaccga tgaatcgaaa
     7261 agacgaacat gtttcactag ctaaagcctt tcatgataaa caaaaaaacg aatttgactt
     7321 cgtacggatc atccataacc ctttgccgca aatagcagtt tctgatgtcg atctaagtac
     7381 acaggcagtt gggtttacac taagcagccc attttatatc aatgcgatga ctggcggaag
     7441 cgaaaaaaca aaaaaaatca accaagatct tgcaatcgtc gcaagagaag cagatttgat
     7501 gattgccact ggttcagtca gcgctgcctt gaaagtccct tctttagccg atacttatac
     7561 gattatgcga caagaatatc ctcacgggaa aatcattgcc aacatcggtg cagggacttc
     7621 tgtcgaaaga gccaaagaag ctatccgatt gtttcacgca gatgccttac agattcattt
     7681 gaatgcacct caagagttgg ttatgcctga gggagaccgt gattttacca attggaaagt
     7741 acttattcaa gaaactcaaa cagccattga cgtccccctc atcgtaaaag aagtcggttt
     7801 tggcatgaca agagaaacgc tcaatgatct agctgcttta ggagttcata cagttgatat
     7861 cagcggtcga agcggaacaa gcttcaccca gatcgaaaac gcccgtcgct ccaagcggga
     7921 actgagttat ttagctgact gggggcaatc gacagtctct tcattgcttg aagcaaatga
     7981 agcagacact tctatggaaa tcctagcctc cggaggtatc cgcaatgcct atgacatatt
     8041 taaagccctt tgtctcggcg caaatgccgt gggtacttca ggaaccgtac tgacgcatct
     8101 gatgaatcac ggcgtagaag aaactattat actgatgaag caatggcaag aagagcttcg
     8161 tctgctttat actatggtcg gcgcaacgaa taccgcggcc ctacaccaac aatctctgat
     8221 cttttccgga ccagtgaagg attggtgcga agcaagaggg atcgacttag tgaagtatgg
     8281 aagacgcaca gaaaagagcg tgggacaaaa gtaaagaaca cttttgtctc acgctctaaa
     8341 cacgtacaaa cggcggaagt aaaagcaact ccttcggaaa taagctgaaa ttcacaaaaa
     8401 tttgaaaagc aattttcgtg aattccttct tatttcttgg agttaaatgc ttctgtccca
     8461 gcctcttttt tcgaataaat gatgtttaat cagctgtcct tcggtattcg ttcaatccgc
     8521 tgatttcaca acctctccac taaacagtgt actaaagctc ttttaaggtt gttttccgat
     8581 ataggcaagt atgcctccgt ccacatacaa gacatggccg ttaacgaaat cagatgcgct
     8641 agaagccaag aagacagatg gtccagccaa atcgattggc tcgccccagc gtgctgctgg
     8701 cgtacgtcca acgataaatt gatcgaatgg atgacgttca ccgttttctt gtgtttcgcg
     8761 taatggagca gtttgcggtg tagcaatata acctggtcca attccattac attggatatt
     8821 gtattgaccg tattcagaag cgatgttttt cgtcagcatt tttaatccgc cttttgccgc
     8881 agcataagca ctgaccgttt cgcgtcctaa ttcactcatc atggagcaga tattgatgat
     8941 tttaccgccg cctttttcga tcatatccgg aataactgct tttgaaacga tgaatggggc
     9001 atttaaatcc acgtcgatta cttggcggaa gtctgccgca tccatttcga tcatcggtgt
     9061 ccgtttgatg atccctgcat tgttgactag aatatcaact gaacctacat cttctttgat
     9121 ctgttgaacc atcgcttgaa ccgcttcttc atcggtcaca tcgcagacat aaccttttgc
     9181 atcgatgcct gcttctttat aatgcgctat accttcatca actgattctt gattcaagtt
     9241 attaaatacg atttttgctc ctgctgtagc taaagagcgt gcaatctcaa atccaatacc
     9301 atagacagca ccggtgacta atgctacttt tccatctaaa cgaaacatat ccatactaaa
     9361 ttccatcatt ttctctccct tatgcttatt ttaattgatc catttgcacc atatccatgt
     9421 ctgtataggt gatattttct ccgcacattg cccagatgaa tgagtaattg cttgtaccta
     9481 cacctgaatg gatcgaccag cttggcgaaa taatggcttg ttcattgtct acaactaaat
     9541 gttttgtttc atctggtttg cccatcatgt ggaacacacg tgtatcttca ttggcataat
     9601 caaagtagac ataagcttcc atacgacgtt catgtgtatg acaaggcatc gtattccaag
     9661 cgcttcctgg ttctaaaatc gtataaccca tttgtaattg acagctttcg catacatttg
     9721 gatggatgta ttggtagatt ttacgttcat tcagtgtcag gccttcacca gtctccattg
     9781 gttttacttg gtcgatactg atttttacat ttggatattt atgatgagct ggaacagaac
     9841 taatatagaa tttagctggg ttttcagcat cttgagaaga aaatacaaca tgtcgtgttt
     9901 cttttccgat atagtatccg tcttgttttt tcatttcttc tttttgccca tcgatctcga
     9961 tgaatcctgg tccaccgata ttgatcacac ctagttcacg acgctctaag aagtaatcaa
    10021 cacctagttc tttgtctaat gtgatttcta attctttttc agtaggtgtc actccaccaa
    10081 agatcattcg atcgttgtgc gtataagtca aactgatttt ccctggttca aatacctttt
    10141 ctactaaaaa ttcttttctc aattgttccg ttgaataatg acggatatct tctggactgt
    10201 gtgtataacg tgtttccatt tcttgcattt tcttctactc cttctatcga atgatttttc
    10261 cagagccgca tgctaaaaat tgtgcgactt cttcttgact aaattggttg cagtccccat
    10321 ggatcgtatg cttcaaggca gaagctgccg tagcaaaatc aatgatttct tgcggagaca
    10381 tattggataa aatcccatgg agcacgccac ctgcaaaagc atctccgcca cctactcgat
    10441 ccacgattgg ggtgattcta tggacttggg attccacata ttcatggttc atccacaatg
    10501 tccccttcaa ttcattttca cttgctgaat gaacggtacg cttggtcgaa taaaacacct
    10561 gaatattcgg gaacttttcc tgcatttttt gataataata aattagaggt tgttcttctg
    10621 cttctggcgc tttctcgata cctaacaaat aaagagcatc catttttcca gctgagcaga
    10681 tatctactaa aggtaaaagc tgatggatta cttcgctggc ttcttgctgt gtccataatt
    10741 ttccacgata gttgatatcg aagctgatca ggcagccagc tttttttgca gcttctatca
    10801 cctgtttcgt cattttttgc cattttttag acaaagctgg ggtgattcct gaaacatgaa
    10861 atatatctat tccttggaat aactcctcta gcgaccattc caaatgatcc atttcagcaa
    10921 aacttgatcc ggcacgatca tagatcactg aggcggcgcg ttcgccacta ccagtttcca
    10981 tataataagt acctagtctt gatccaccag ataatacctg tttcgtatct actccataac
    11041 attgcaagtg ctttttgact gcttttccca atgcgttatc aggtactttt gtagcaaaaa
    11101 tcgcttcatg tccgtaatta gctaaagaaa ttgctacgtt tgcttcccct ccgccataat
    11161 gggcagcgaa tcgttcactt gaatcgatac gggttccctg ctctgtggac aatcgcaaca
    11221 tgatttctcc gagtgtcact acttttccca taagcagcta cctcttgatt tctttcattt
    11281 ttgctgcata ctgttgcgcg acttccgtta ctttgtcaaa atctcctgta gctgcgggtg
    11341 ctaaaaggtt cccacctact ccgactgtta cgacaccagc atcgaaccac tctttcatat
    11401 tgtccagact cacaccgcca gttggcatga tattgacttg aggcatcggc gccttgaagg
    11461 cagaaatgat acttggtcca taagcgcttc caggaaatag tttgacgata tctaccccgc
    11521 ttttcagcgc tgttttgatc tcggtaatag tcatacatcc tggtaagtaa ggaatttgat
    11581 acagattgca catctctgcc gtttcttgat caaaggaagg gctaacaata tactctgcgc
    11641 cagctaagat agccaagcga gctgtgacag cgtctaatac tgtgcctgcg ccaatcacta
    11701 tttctggttg gtctttgtag aatgacaaaa gttctttgat tacttgatct gcttgaggga
    11761 ctgtaaaagt caactcaata ccagttagac cacctttgat gattgcgtgg caagcattca
    11821 aagcttcttc tttgcttttt ccgcgtacca ccgcaatcac tccggcgttt ttcaatcgag
    11881 ataaaatatc aatttttttc atcttctcca cctatttcct atttagaaat tttattttat
    11941 aacttggaaa tctataccca tattatacgg cagaaaagaa aaattgcaaa cgcttttttc
    12001 attatttttg aattctttgc tcgatttcgc tttttgtttg cttgagggca ctgattattt
    12061 gttcttttgt ctcatctgtt aaacgatatt tcggcatact tacactaaat gcaccaatga
    12121 tttgttcgtt tttcacgatt gaggctccta tacaaaaaat atcacgttcg atttcttcat
    12181 cgtcaaatgc gatgtgggtt tcttttaccg ttttgatttc ctttttcaac cgtaatgcat
    12241 tggtgatcgt atattctgtg taagggatca acggatgctt ttcaagataa tcagtgtact
    12301 gctcttcact gaattctgct aatacggctt tgcccatcgc agaattgtat aatggtcggg
    12361 tgattccgat ttttgaagac atgcggatcg tttgatgttt tggatctagt ttgttcacgt
    12421 acaagatctc attgttgtcc aataccccta agtggatagt ctcatcgatc ttttgctgca
    12481 gctgttctaa aaacggtaaa gtcaactcta ccaaatcgat ttgttcaatg tttttattcg
    12541 cgtagcgaat aagctttgca cctaaacgat agtttttctc tgaattttta tttacgtagc
    12601 cgatcatcaa caaagtatct agtattttca agactgtaga agaggtcatt cccacaccct
    12661 ttgcgatcgt ctgcaaagag acatcttggt tttccgctaa atagtctagt attttagagg
    12721 ctttgatcaa tacagttcca taaggctttt tattttccat ataatattct cctatttctt
    12781 tataagaaaa attatagcgg aaataccatc aatttaaaag agatatccag atataaacta
    12841 ccatttctct ttacaggcaa tttaatgttg aagatccgct tcgtttttac atcaaacttt
    12901 gatataaagc aatgatttct tctactgtcg tttctcttgg atttcctggt gtgcatacat
    12961 ctcgcaaagc atcttctgca attgctggaa tatctgcttc ttttacacct aattcagaaa
    13021 tggtttctgg gattccaacc gctttgctca accgatcgat ctcaccacaa gccgcttctc
    13081 ttacatcttc taaagacatt tcagctgctc ctttaatccc tagaacaaga gcgatctcac
    13141 ggtacttttc tccagtatat tctttgttat atttcatgac agttggcaaa agtgctgcac
    13201 acgctacgcc atgaggaatg ttgtaccatg cacttaacgg gtgagccatc ccgtgtacca
    13261 gtcctaaacc gacatttgag aagcccattc ccgcaatata ctgtcctaat gccatttttt
    13321 ctcgcccatt ttgatcgcca tctacagaag cttccaaaga atggccgatg atttcgattg
    13381 cttttaaatg cagcatatca gtcatttccc aagcgccttt tgtgataaac ccttcaatag
    13441 catgcgtcat agcatccata cctgttgatg cagccaattt tttcggcatt cccatcatca
    13501 tatcgctgtc gatgaacgcg acgattggga tatcatgagg atcaacacaa acaaattttc
    13561 ggtgatttac ttcatctgtg atgacgtagt tgatcgttac ttctgctgct gttccagaag
    13621 tcgttggtat agctaatata ggtaaacatg gatttttcgt atcagcaaca ccttctaaac
    13681 taatgacgtc tgaaaactct ggattcgtca aaataatccc aatggctttt gccgtatcga
    13741 ttggcgaacc tccaccgatt gcgatcaaac agtctgcctc tgcctcttta acaaaagcca
    13801 ctccatcttt tacattttgg atcgttggat taggtacgat cccgtcgtat aaagcataag
    13861 cgattgctgc tttatctaga agttctgtta cttttgttgc tataccgtgc tcgatcaatc
    13921 ctttgtcagt aatgacagct gctttgccga atcctcgctg ctgaaattca gggacgatcg
    13981 actcaatcgc tccttttcca aaatacgatg tttcatttaa aatcattcga ttactcattt
    14041 tctattcctc ctaatttgta ttatttttca caataaaaac gaaagaaccc caagcaaccg
    14101 gttgcccagt tgcttgttgc tacagttcaa agactggcat caattcccaa ctgactggag
    14161 aggcatctgg atttgtctcc atgatatctt ccatgtactt ccaccatttt tgattgatag
    14221 atgtatttgc cattttttcc cataactttt catcttcgat cactaagtaa ccaaataaca
    14281 aacttgtact cggatcaaga tagatcgaat agtcgatgac cccgtgttca cgaagtgctg
    14341 tacgcatttc tggccaaagt tcatcatgtc tttttttgta ttcttcttgt ttttctgggt
    14401 aaactttcat gcaaaaagct tttttcacca tgtcagacac ctgctttcgt atgaacttcc
    14461 tccaaataac ctgcttttgg agtgacacca aacgcatctg ctaaatgcca caggttttcg
    14521 tccgtgattg tttgcaagat tggtccttgc gctttgacgt acgtatagac ttctgctgcc
    14581 ttttctgctg tctcgatcaa accaaagact tcatccatgt cttttcctga cccgtagatt
    14641 ccatgttgtg gccataaaac caatcgagtt tctttcattt tttcagctgt tgcttcccca
    14701 atctcatttg ttcccggaac catccacgga atgatggaaa ttccttctgg aaaaacaact
    14761 aagcattccg tacacatttg ccatagcgta cgactaaagg aacgttcgtc taattcatgc
    14821 gtaaaactca tcgctaatag atgactagca tggcaatgca taacgatccg attatctggg
    14881 tctacttcta aacgggcgat atgactcatg aaatgacttg gcaactcgct tgtaggaagg
    14941 cattcgtttt ccagccccca caaaagttcc agtgttttgc catcttcagc aactcgaacg
    15001 attccaagat tctcggcagg ttcgctcgct acatttttaa aatatcttcc tgaaccagtt
    15061 acaataaaat agcatcctgc taaacggctg gcatcaaaaa tcatcgagat gcggcggatc
    15121 actttttcag gatccaagaa cggtgtgatc tcttcttctt ttagtaaata agaaatattc
    15181 ccgccatttc tttcatccca tcccaagcga taaagattcg acgtcgtttc gatcatttct
    15241 ttcacataag gcgcttgtaa aatatctatt tttttcaata tagccacact cctattttct
    15301 taattccaac acgtcttttt gatattcctg gacttcttcc agccaatcca aaccaactgg
    15361 aacttgattt ttgtaacaga aataattcca tacatcggca tacgggaaat ctttcaattc
    15421 ttccgtataa gccattcttg aagtaaagtc aaaagccagc tccatttctt tcaatcgctc
    15481 aactggttcc agcatagctt tcagcagtgc tttttgtgta tttctcgtac cgatgaccca
    15541 agcagcgata cggttgatcg ttgcatcaaa gaaatccaga ccgatatttg ttttttccaa
    15601 caattcgtta cggactaatt ctttagcgat ttcctgcaat tcatcatcca tcaaaaccac
    15661 atggtctgaa tcccaacgca ctggacggga aacatgcaac atgattcctt tgctgaataa
    15721 agctaatgaa gataatttat tggaaatcac ttctgttgga tgaaaatgtc cagcatctag
    15781 acaaacaagt ttgtcacgag tcaacccgta acccatataa aattcatggg aaccgactgt
    15841 ataagcttca gcgcctaaac caaatagttt gctttctact gcatcttgta cataccgttc
    15901 atctaactct tctgtaaaaa tttcatcgag tgattccatt aaacgttccc gtggggtaaa
    15961 acgatcaatc ggattatctt tcatcccatc aggtacccaa aagttattga tacttgtttg
    16021 cccagtttct ttcccaaagt actccgcgat tttgcggctt cttttcccgt gttcgatcca
    16081 gtattcacga acttctttat ctggatgagc caatgtaaag ccgtctttca tcattggatg
    16141 cgaaaagaaa gtcggattga agtcaagacc gatttcttct ttttttgccc actctaccca
    16201 tttttcaaaa tgtttcggct caatttcatt tagatcgaca gattcttctg tatccaaata
    16261 gatagcatga aggttcaatt tgtgtttgcc cggtatcaac gaataagcct tctccagatc
    16321 ttgtctaagc tcttcaggtg ttcgtgcggc tcctggaaaa ttccctgtcg ccatgattcc
    16381 accggtcaat tcgccatccg gtgtcaagaa acctttgaca tcatctcctt gccagcaatg
    16441 catagaaatt ttgatgtcct gtatcttttc tagtacagca tctgtatcta caccgatagt
    16501 tgcatatttt tcttttgctt gttcgtaatt tctttcaatc gtgttcatac gattactcct
    16561 ttgattttat tcacgtacca ttccatcgtg ttttgcttat tttcatctgg gtaaaaagtt
    16621 tcacatggaa acgactgttt gattacattt cttgcttgtt ctatattcga aaactgacct
    16681 gtactgagca tttgcatggc aacattcccg attgctgtgg cttcactagg tcctgcttct
    16741 acaatgattc cggcataatc tgctgtcaat tgattcagta gacgattatt cgatccgccc
    16801 cctacgatgt gcagcttttc caatttcgtt ccagttaagt gttctacttt gatcagctct
    16861 tgtgcgtaac aaagagcaag attatcatac acacaacgag cgatagctcc cgatgagaca
    16921 gggactggct gctctgtttc aaaacagtat tgctgaatct cttctatcat atttggggga
    16981 ttcaaaaatc gagggtcatt gatttccatc acttgctgca gcggtttttc ttttgcagca
    17041 agctctgcca attccgcata actgtactcg tactcttgca ttcgggcaac ttcctggatc
    17101 aaccacatcc ccatgatgtt tttcaaaaaa cgcaccgtgt tatgggcacc ccattcattc
    17161 gtataatttt cagaaaaagc attgcgagaa acattggcga tctttgtttc aatccctaaa
    17221 agcgaccatg tcccactgct gatataaccc caacgttctc cttgacctgg cgttcctaat
    17281 acagctgacg ccgtatcatg acttgctact gtgataaagg tcacatctgg caaatcatat
    17341 tttgaaaatt gttctttttg cagatctcct agtatcgttc ccggctctgt caatggtgga
    17401 aataattcct tcgagacacc gattttttca agaagttcta tttcccattc ttttgtccct
    17461 gcattcagca gctgcatagt agatgcatta gttttctccg tcaccatgtt accagtaaag
    17521 acatacccta aataatcagg tatcagtaac aatttatgtg cttttgccaa attttctttt
    17581 tcttcagcaa acaattggaa caaggtattg aaaggctgga tctgaatccc tgtctgctca
    17641 tacaacttat ctagagaata gatttcagaa aactgttcta ctgctccatt ggtacgcttg
    17701 tcccgataag caatcggatc atttatccga tccccttgtt catcaatcag acagtaatcg
    17761 actgcccatg tatcgatccc cgcaaaaagg cgatcaatcc ctgtctgctt taatttttca
    17821 aatccaatca aaatctcttt gataagataa tcgatcttcc agcgatcgtg accgtctttg
    17881 ttccgaaagc cgtttttgaa ccggtgtact tcttccaaga tcaaagaacc ttttgcatcc
    17941 ctcgtacttt tcattagtcg tccgcttgat gcaccgatat cgatggcaac aaatgttgga
    18001 ttcatttctc cacctctttt actttattgt tgattagcac tttattaata aagcgttatc
    18061 atttattgac aatgctatca taatacagaa tcttatattt tgcttcacac tattttggta
    18121 tataattgca ttatcttgac aagttgggag tgtttacatg gattactggg aaaaagccaa
    18181 ccactcatgg tcggaagact caaaaagagt gatcttgacg cctacacaaa gaagcaggaa
    18241 tttatttttt tatatccaag aaatcggtta cttcaaagca ccaatccctt actatactga
    18301 acgagcacat ttaccttctt acttgataaa gtttactttg tctggcaaag gttcgcttac
    18361 atataatgat aatcagcaac agctaaaagc tggagactta ttttttattg actgccaaga
    18421 atatcagcgt tatcaaacga tcagcgaaga accatgggaa atggattgga tccatttgaa
    18481 tggtcagaac gtccaatcat tttatcagga gttcacaaaa gacggccata acaccttcca
    18541 ttcaacagga gatccatcag aaaacaagat ccaccatttg atcacagaat tgatccacat
    18601 gcaacaaggc acgaatgccc gtaccgattt ccagtcctct gtagctatcc acgagttgct
    18661 gaatgaactg attttgcaaa agtatcagct agatttcgaa gaagaggata ttcccgccta
    18721 cgtttacaaa ctgaagcaat acttagacga acatttcaaa gaaacgatta cacttgatca
    18781 attagaacaa caattcttat tgaataaata tcagctgagc aaagattttt ccaaatttat
    18841 cggttctcca ccaatcgatt atttgatcag caaaaaactt tcttacgcta aagaccttct
    18901 tcggtataca gaatttacgg ttcgaatgat ttccgaagaa atagggattg agaattttgc
    18961 ttactttagc cgcctgttca aagcaaaaac aggactatcg ccgagagact ttcgtaaatt
    19021 cagctgattc atataatatg tacgtcattt tttcaaaata ttttgaaata tatagataaa
    19081 aataaaataa ccgcagtttc tgcttctatg taaacaacag cagctactgc ggttattttt
    19141 tagtgatcat tctcattgtt catcttcaaa atgttcataa tgaaccaact gtttctgtgt
    19201 atcacgtttc aaaaattctt taggcgttat gccgaactct ctcttgaatg ctttcagaaa
    19261 atgcggataa gaagaaaaac cacttttact atatacagca ctagagctgc ggtattctcg
    19321 caacaagtgc ttggcataga tcagcttttt cttcattaca tactgatgaa acgtaaatcc
    19381 agtgtacttt ttaaattctc gtgtgatatg gtatttgctg acaaaaaact gcttttccat
    19441 ttcctctaaa gaaagcggat gagataaatt ttttgagata tattgtaaca tttgttccac
    19501 acgctcatta ggaactacag cattctgttc ttgcgcactc tcttcttttt gtacaacttg
    19561 attgagaaaa atcaagtatt ctaccagagc ttgttcatat tttatgtctt caccgtaaat
    19621 ttttttatca ggaggatggt cgataaatga taatttttgc ttcaatactt cctgttctat
    19681 ttgcaataca cgactcctct tctctcccat tggttgaaaa caagccgata gattagacca
    19741 tggcgtggaa cgactttcta gaaaagaagg ggtgacaaag atatagacac gctcgaatac
    19801 atctgaagat tggcggacga tacgatgcaa atcattggaa tggatgagca gcacacttcc
    19861 tgctttgatc gtaaattgtc gtccgttcaa ataaaaagtc gcttctccct ttaatgtagc
    19921 atggatctca tagaaatcat gatgatgata gatcgtttta ttttggctga cgacatcttt
    19981 gacatgatag atttcatact gatcagaaac catcgtattg acttgttcac cgctgatttt
    20041 cttttggagc atgtgccgca cctccttttg ttcacttatg cacaatttta ttataccacg
    20101 ttttttgtaa tcaaaaagag ctgcttttat atactcttat aaaaaagaag agacgtggca
    20161 tcttctagcc ccgccccctc cattctaaat attagttaac caacgtcttc tttacattat
    20221 tcaagaagac ttcttccata acgaatccca tcatcttttg tttcttcaag aaccacatac
    20281 aggtacggac gattcttcat gatgatttct ttgatttttg tgtactcgat cacaccgtct
    20341 cccaaattta ccggcacgat tttttcctgt tcgacacgaa aatctttcaa atgaaccgca
    20401 catatctttt caccaaaacg ttcaaaagct tcttctacca attggacttg ttcttgataa
    20461 tcactacttg tgatcaaatt ggtcggatct aaaatgattc ctagatagtc cgaatctact
    20521 gcttggatca gctgctctac ccttgctaaa gaatacaccg gatgattcaa acctggttct
    20581 atccccacca tcatttggta tttttctcca gcttttacca agcgacggat aacggacaca
    20641 gcttcagcaa atgcttcatc tgtgaaattt tcttccgtat attggatttc cggcaaaaca
    20701 catccagttt ccgaagcaac catcgacgca ccaaaggaag aagcatagcg aagatactgc
    20761 tcgaactttg tcagcaactc ttgtcgtatc gttaaatctg gatgaatcat attgatatag
    20821 caacttaaaa ttcctacggt cacttcttgc ttttctaaaa ctcgtttgac ataatttccc
    20881 atcccagaat tgatctcatt tgcgccagaa ggtatttcag gaaacgaaag acctaaggcg
    20941 agctgcagtg tatgaatccc ttgttctttt gttttttctg caagatcttc tatcgtttgg
    21001 acttcggaaa gatcatgtcc tctaattgta atgttcaaca taactattct cctaactcag
    21061 catattgggt agccacatga cgatttgcgg gaagtaagta atcaaaaaca agacgactaa
    21121 gataacccca taaaaaggaa gaagcggttt cagcattgcc tctgctttta cgcctcctac
    21181 acttgcaccg acatatagtc ctgtacctac tggcggcgtg atggagccta cacacagatt
    21241 catgattgaa aacaacccgt aatgtacagg atcaacccct acacttgtga cgattggtaa
    21301 aaatattggt gtaaaaatca agatagcagg cgccacatcc ataaacatac ccaccagtaa
    21361 taacacaaga ttgactaata acagaataat aaatttatta tccgttacgc ccataatcaa
    21421 cgaactaatt gcctgaggaa taccggtaaa cgccatggcg aaagacatca tggaagaagt
    21481 agccaataag aacatgatcg tccctgacat tgctactgat tgaaacaaca tattaggtat
    21541 atcttttagt tttaccgttt tatagtaaaa catagcaatc aacagggagt aaatgacagc
    21601 tatcgctgaa gcttctactg ctgtaaatat ccccgtcaag ataccaccga taatgatgac
    21661 aatcaataga aggctaggaa tcgcctcttt tattattttt ccagcatgag ccatctgtcc
    21721 tttttcgaca acagggtatt tattcttttt agcaacgatg aatgctacta gcatgattcc
    21781 tagcgcccac aaagcgccta cgagataacc tcccataaac aaagcagaga tcgaagcacc
    21841 accagcaaca agtgaataca tgataaaagc agtacttgga ggaatgacca tacctgtcgg
    21901 agctgacgcg atattgaccg ctgtcgcaaa cttccggtca tatccttctt ccacttgctg
    21961 agggatcaac actccgccaa tcgcggtaga agcggcaatg gccgagctag agatggaacc
    22021 aaataaagca ttccctaaca cattcgtatg agccaaagcc cccggcacgc gcccgacgaa
    22081 caacatcgca aaatccacta gtttcttagc tatccctcca ttattcatga taatgcccga
    22141 gagaacgaaa aacggaatag cgatcagcga aaaactattg atacttccca ccattttttg
    22201 agctgaacta aaagcagaaa catcaaacgg tataactaac aataaagtca tcatcgatga
    22261 gaatgcaata cttatagcaa tcggcatccc taatacaagc aacacaacaa atacaaaaaa
    22321 taacactaat ccggcttcta ttgccatttt attcctccat ttttatattt tttcttgaat
    22381 tgctggcttg ttgcttaatg tatcgattag attgatcagg ctatagataa tgatgagtac
    22441 ccccgataca ggtactgaca gataaacata tcccatagga atacttaaag aaggcgagat
    22501 ctgccccatt gtcaatgtga cagcattcca tccgccatac accatgatca tcgcagcaaa
    22561 taccagaaat aaactttgaa caacaacttc taaaatttta ctttttttgc cagttaattt
    22621 ttcacttaaa accgttacgg ctaaatgtgc tttttgtccc acaacatacg cggaagacaa
    22681 catggcaaac cagacaagac cgaatcgaac aagctcttcc gtgaccgtat ttggatttcc
    22741 taaaaccgtt ctggaaaata cttgccataa aacaacagcg accatcccaa caagcaaaac
    22801 agctcccagg atctctaata ccttgtttaa aatcattttc atcacagcta cgctccttgc
    22861 tcttggatct tttgataaag gtctttgtag gtaggatcgt ttttgaatat atcatgaagt
    22921 ggggcaactg cctctcgaaa cggtgtatta tctacatcat tgaattggac tccatgttct
    22981 tcttgtgcta aagctttttc ttcttcaatt gcttttgcaa aagcttcaat ttcaaaaacc
    23041 gttgattctt tcgctgcttc ttcaatggct ttttcctgct catcagttag ctgttctttg
    23101 atggcatcat tcatgatgat gatatctggc acacgtgtat gctcatcata ggaatagtat
    23161 ttagccacac caccatgtcc agctgtatac aagacaaatt cattgttttc tgatccgttg
    23221 atcaaattgg attgaagaga cgtgtacact tcatcacttc ccataggtac gggcgaaccg
    23281 cctaaaagct ctaccatttt gatagcggtt tcgctttgca tcacacggat cttctttccc
    23341 ttcaaatctt ccggcgtatg gatcggaccg tctaccatat aaaagctgcg ctgaccagaa
    23401 tcataatagg tcaatccggt aaatcctaaa tttttggtcg attggtagat tggctccatt
    23461 atttcttcat tatccatcac cttaaagaaa tgttcttcat cattgaatag ataaggaact
    23521 gaaaaaatcg aataatcttt agagaaactt tccaacgctg aggcacttac ttttgtgaaa
    23581 tctactgctc ctgactgtgt caattcgatc agttcacgtt ctccacccag ttgtccatct
    23641 gggaaatact ggatctgtac ggaaccatct gttttttctt ttactaaacg cccaaattca
    23701 ttcattgcat caattactgg ctggctgttg cttgcataag caaagcgcaa ggtaactatc
    23761 tcatttacat ctgcatctgc gacagttgcc acactagaat tatttccgca gccgcttaga
    23821 cctagcagac ctaggctaag cgaacttgct gcaaacagca gtgatgtttt tttcatatgg
    23881 gtttcctttc ttttaacaat aagagacgag caattctgtc aaacaaagca ccgttaacga
    23941 ctgtccataa ggcattgctg tgattccgat attccgataa aaatctaaac tgtcccccat
    24001 tcctgtcccg attgaaacgt tttgaacttc tcctttttca tcgatttgtt caagcaatcc
    24061 ttggatagca cgataagcga cttctttata ttcttgtggt aaataacgtt tatggaccgc
    24121 tttcaaaata ccgtaagcaa atcctgcagt tgcagaagat tctacgtaag aagaaggatc
    24181 atccaacaac gtatgccata aaccgctttc agactgataa gtttttaatg cacgcacttg
    24241 tgcttcaagt gtttcgatca ataatttgcg caatgcatct tctttcgata attctaagat
    24301 ttcaatgatc tctggaatag caatcgtcaa ccagcagttt ccacgagccc aaaatgcttc
    24361 ggcatagtta tgatttcctt caaacgtcca gccgtgatac cacaatcctg ttcgtttatc
    24421 catcaagtat ttgatatgga tcataaactg gtaacgagct tcttctaaat attctgggcg
    24481 attcaacagt ttcccgattt ttgccaaagg aagcactgtc atcatcaatg tatcgtccca
    24541 caactgattc ttattttccg gtccatatgt ggcatgctgt aacccgtctt catttgtccg
    24601 aggcatgtca ttcatgaccc attctgccca ttgttctaaa taaggcaaat agcgtgaatc
    24661 tttcgtgtct tcatacaagt aagccatagt caatagcggt gccattgtat tgacgttttt
    24721 gggcggagcg ccttcttcca tacgatcctc aaaccattca ttgaccactt gataagcgcg
    24781 aggatttttt gtcatctggt aatttttata aataccatat agaccgacac cttgtggcca
    24841 attccacact gtccagcttt tgtcatctac tttcagtcca tcaaagtcca acaaaaattc
    24901 accattatcg tctttgatcg ttgtcaggtt ttcaacgagc aggtcaattt tttctttcac
    24961 taattcttta ggtacaaaaa tctcttcttt aataatcatc tttacgccac ctttttaaac
    25021 gctttcattt tatatcctta atatagcagt aataccgtcc actataaata tcgctttttg
    25081 aacaaaaact gtctaaaatt gacctaaatg ttaaataaca aaagtaactt tcagttactt
    25141 ggaaattaaa aaaagaactt ggagaaacaa cgttttccaa gttctttttc atctattttc
    25201 ttatcttttt tctacgcttt cttttgctta tccgttgtaa gcatcaatga tgatctgttt
    25261 caattcagag atcaatggtt ctttcggatt agctgtcgta cattggtctt cgtaagctaa
    25321 ttcagccata cggtctacgg ttgtatctag tgtttcttgt gtgactcctt gagcttttag
    25381 actcatgttg atgccgacag attttcctaa ttcataaaca gccgttgcta aagcttcgac
    25441 taattcctct gttgtttcgc ctttcagacc taagaatttt gcgatatctg cgtaatctgt
    25501 atctgcacgg aaatagtcat atttagggaa catcgcatgt ttttgtggat cttttgcatt
    25561 atagcggata atatgcggta aaagaatcgc atttgtccgt ccatgaggga tcccgtattc
    25621 cccgccgatt ttatgtgcga ctgagtgaca aatacctagg aatgcgttag caaacgccat
    25681 accagccatt gtagaagcgt tgtgcatttt ttcacgtgat tccatatcag gtgtttttac
    25741 tgatttttct aaatagtcaa agactaactt gattgcttgt aggctcaatc cacgtgtata
    25801 atctgaagcc atgactgaaa cataggattc gattgcatgt gttaacacat ccatcccggt
    25861 atctgctgtg acagatgctg gaactgacat gacgaattga ggatcgacga ttgcgacatc
    25921 tggcgtcaat gcataatctg ctaacggata tttcacatgt gtttcactgt cagtgatgac
    25981 cgcaaatggc gttacttcag aaccagtccc tgatgttgtc gggatacata cgaattgtgt
    26041 tttttctggt ttgtcgatct tatatgtccg tttacggata tccaagaatt tttgttttgc
    26101 accaaagaaa gatgtgtctg ggtgttcata gaacatccac attcccttag ctgcgtccat
    26161 tgcagaaccg cctccaagag cgattactgt atctggcttg aagtctgcca tcagtttcgt
    26221 tcctgcgtat acggtatttg tcgatgggtt cggttcaaca tctgagaaga cttcgatctt
    26281 cacatcattt ttacgtcgtg ccaatacttc acgaactgta tcgcagtaac cgaattggac
    26341 cataccaggg tcacaaacga tcatgacacg ttcaacgttt tccattttct ctaagtacaa
    26401 tagagagttc ttttcaaaga agatttttgg cggtaatttg aaccattgca tgttatttct
    26461 ccgtttcgct acagttttga cattgattaa gtttacagca gagacgttac gagaaacaga
    26521 gttcttacca taagaaccgc atcccaaagt caatgacgga atcatttcgt tatagatatc
    26581 accgatgccg ccttctgcag atggtgtatt cactaaaata cgacaagctt tcattcgaag
    26641 accgaatttg acatgcaact cttcgttttc tgaatggata actgctgtat gtcctaatcc
    26701 tccaagattc aacatacctt cacacaaatc aaacgcatgg tccgtgttgt tagctttgat
    26761 catggcaaga actggagaaa gtttttctct tgatagcgga taatctgggc ctacgccttc
    26821 caattcagca atcagcattt tcgttccttt tggtacactg attcctgcca attttgcgat
    26881 ttccatcgca gaatgtccaa cgattgatgg attgaccgca tattttcctt cgttcatcac
    26941 tgcatcttct aatttttgca attcatctgg ttttacgata taaacttggt gtgcttggaa
    27001 ttctgctttt actgctgcat agatttcttt atccacgatc acagcttgtt cagaagcaca
    27061 aatcattcca ttatcaaatg ttttcgaaac gatcaaatcg ttcactgcac gtttaatttt
    27121 tgctgttttt tcaatataag ctggtacgtt acctggacct acacctaaag ctggttttcc
    27181 tgtagagtaa gcagacttga ccattccagc tccacctgtc gctaagacga tcgcgatacc
    27241 tggatggttc attagcatag aagtcgcttc gatcgaagga tgttcgatcc attggataca
    27301 attttcagga gctccggcag cgatagcagc atcacgtacc actcgtgctg cttctgcaga
    27361 agatttttgg gcacttggat ggaaagcaaa aacgattgga tttcttgttt tcaacgcgat
    27421 cattgatttg aaaatcgttg tggaagtcgg attcgttgtt ggagttactc cgcaaaccac
    27481 accgactggt tcagcaattt caatcaaccc tttttggaca tcttcattga tcacaccaac
    27541 tgttttatca tgtttgatat tatgccaaat atattcggac gcatacatat ttttgattgc
    27601 tttatcttcg taaacaccgc ggcctgtttc ctctacagcc atcttcgcta atggcatatg
    27661 ttgatccaaa gctgccattg ccatttcatg cacgatatgg tctacttttt cttgatcaaa
    27721 gctttccatt tctttcaatg ctgcatttgc tttttctgcc aaatcatcaa tcattgcttg
    27781 tacgtcaatt gtcttcgttt tttctttttc tattacttta gccatcatta tcctcctaat
    27841 gggtcatggt tatttgttaa ataattcaca tgtatatcat acaatattta tttttctatg
    27901 taaacacttt tttgtgattt ttttcacagt tatttttaaa tataaaattg ctttatttac
    27961 aggtgtttgt atagaaaaat atataaagtt atttattatt atttatatga cacacccgat
    28021 tattcaatat tgagcaatat atcccatgct cttatctctt tcttttttga ataaaaaatg
    28081 aggtaggaca tctttctgcc ctacctcgtc tttaaataag ctttgatact tatttttctt
    28141 ccttctcttc tgtctcttcc agcgtttctg ttgtttcagt ttttacttca actgtctcag
    28201 ctggttcttc tgttgtttca acagtagtcg ttgcatcatt tgttacgaca gtccctggtt
    28261 tgaccgtttt gattgctgca cgatcgtact caaggaagat cccttcgcag tctaaaacaa
    28321 ctgtccgttt gtcattgtcg atttctgaaa caactccatg taatccgccg atcgttacga
    28381 ctgaatcacc tggtttcatg ctatctaaca agttttgacg ttcttgctgt tgtttctttt
    28441 gcgaacgtga cataaaaaac cacattgcta atagcgcgat aaacatcaca agcattggta
    28501 atgttcccat ttttttcacc tcactaattc tgtatctaca aaaaacactt tagcagaaaa
    28561 atgctgataa cactagtttt ttacttaatt tttaaaagat tttcctttag aagctttttg
    28621 cgttttcttt attgaacccg tattcttcaa aaaaggcttc tctaaattca agcaaattat
    28681 catccatgat tgcttgacgt acttgtttca ttagattcaa caagaagtat aaattgtggt
    28741 aagaagtcaa acgaatccca aatgtttcat cacatttgat caaatggcgg atataggcac
    28801 gtgtatagtt tttacatgta taacaatcac atttctcatc aattggacgg aaatcatggg
    28861 catatttcgc atttttcact actagacggc cttgagaagt catacatgtg ccatttcgag
    28921 cgattcgagt aggcaagaca cagtcaaaca tgtctactcc acggataaca ccatcgatca
    28981 atgaatcagc agcaccgaca cccatcaagt aacgtggttt gttttcgggg atcaacggtg
    29041 tagtgaattc taatacacga ttcatttctg atttaggttc cccgacagat aacccgccga
    29101 ttgagtaacc agggaaatcc atcccgacta gatctttcgc gctttgacga cgaagatctt
    29161 caaaccctgc tccttggatg attccaaata atccttgacg gtctggatta gcatgagctt
    29221 tcaatcctcg ttctgcccat cgagacgtac gttctactga ttttttcacg tagtcgtaac
    29281 tttcatcgaa tggtggacat tcgtcaaaac tcatcatgat atcagagccc aatttatttt
    29341 ggatgttgat tgctttttct ggtgataaaa acatacgcga accgttcaag tgatttttga
    29401 aatgcacgcc ttcttcttcg atattccgca tatcacttaa tgaaaaaact tgaaagccgc
    29461 ctgaatctgt taaaatcggt tgatcccagt tcataaattt atgcagtccg ccagcttctt
    29521 ctactaagtc ttcaccaggg cgtagccata aatgatatgt attacttaaa ataacgccag
    29581 cacccatttc tttcaactct tctggggaca ttgtttttac tgtagctaat gttcctactg
    29641 gcatgaacat tggtgtgggg aaagttccgt gtggcgtgat caattcccct aaacgcgcgc
    29701 cagtatgttt ttctttcttg attaaacgat agcgaatggc aggttgcgtc attcatttcc
    29761 ctactttcta tataaaatat cttgacaact cccaccatca taatcgtttc aagtgctttt
    29821 tgcaagttcc ctcggcttcc ttctacttta tttaatattc ttttttttat ccttttttta
    29881 tgaataacaa aaaaaagaaa aacattatga cagaaatatt ttttgataca tggtatattg
    29941 gtgttattaa agaaaaggag ggaaaaatca tgcaatcttc tgcagaatta aaacgccaag
    30001 caaaagagag tctaaaagga cgttggggac aagctgtttt actaaatcta atccctacat
    30061 tgattggtat tgctactatt cttcttatta tgattcctat tatcttgttt gccaccacca
    30121 tatatgatgg atcagcgagc gtctcagaag cgacaacttc aggaggcggt agttcaggag
    30181 gcggtggcgg aattatttcc agcatcatta gcgctttgtt catgagcggt atttcttgga
    30241 cttatcttga tattttacgt ggacaaaaag atcagatcga accattcaaa gatgcatttc
    30301 gtggattcaa aggtttgttt atcggcggag tgatcagctt agctgtgctg atctcgatct
    30361 ttacaacatt atggggtttg ctattagtta ttcctggaat cattaaaagc tattcttatt
    30421 ctcaaagcta ttttatttac tacgatatcg tccaagaaac aggacaaaca cctaaagtac
    30481 tggatacgat cactgcaagt cgaaaattga tggatggtta taaaggacaa cttttctggt
    30541 tagatctttc atttatcggc tggcatatct tagcattagc aactgctggt attggttatc
    30601 tatggctcag cccatacatt tctgcaacaa aagcagcatt ttatgaacaa ttgccaaaag
    30661 atatttaaaa gagagtgtga cttgtgaccg agggggtggc aggagaacac cgtttatcga
    30721 gaggcagtaa aagaaacatt cagcccatta gcaaaaagga ggtgtgacaa cattcatgtc
    30781 acacctcctt tttgtatagt tccagtaaaa agaaaatcaa cttagaaaca gttaatcatt
    30841 cctcttcttc tgcttccggt aatcgatgga acttgatcag ttcattcacc gcgattggta
    30901 cgatagataa aatccctacc aagaaccagt gattcaaact gatgtgtact aggccgaata
    30961 gttcttgcgt aaatggcagc aaagcaacag ccgttgtaat agctaatgcc agtaacgcca
    31021 tttctaccaa agctttattg gtcgctaaat tgactttgaa gatcgattgg ctgctgcgta
    31081 cattgaacac atgcagaatc gatgaataag ctaagatcaa gaatgcaaca gtttgaccga
    31141 catgctgact tgcgtctacc catgggctga tagtatcgac ataactacct aaataataac
    31201 caaacaaggt tacgatggtg aagacccctg catttatggc aattttttgc aataaacctc
    31261 gagcaaagat cccttcattt ttcggaatgg gtggctgatc catgatgccg tgttccgctg
    31321 gctctcttcc taaagcaaat cctggaagtc catcagcaac tacattgaca aacagtagtt
    31381 gcacagctgt aaatggtgct ccccatccta acagcatcgc aatcaaaaca ataaagatct
    31441 ccgaaatatt acagcttagc aaaaagttga tagctttacg aatattctga taaaccgaac
    31501 gtccttgact gacagcgtct acgatcgtcg caaagttgtc atccgtcaaa atcatatctg
    31561 cggcactttg cgccacatct gttcctgtga tccccatggc acacccaacg ttacttgctt
    31621 tcaacgctgg tgcatcattc acaccatccc cagtcatcgc tacgactgca cccgtacgtt
    31681 gccaagattt gacgatccgg atcttgtctt ctggcgttac ccgagcatag acagagagag
    31741 ctttgacacg cgcatctaat tcttcgtctg ataattgatg cagttctgat cctgaaagtg
    31801 cttctgacgg atcattcaag atacctaatt ctttcgctat ggcactggca gtcaccacat
    31861 gatctccagt gatcatgact gttttgatgc cagccttttt cgctcgcttg attgctcctt
    31921 tactttctgg acgtggtgga tcgatcattc cgatcagacc aatcaaacgt aagtcttttt
    31981 ccagtgcttc agaagaaata tcttgcggtt cttcgtcata agtagcataa ccaacagcaa
    32041 tcactctcag tgcccgttta ccaaagcgat cattaacgat cgccgcttgt tctacatcgc
    32101 ctgaactaaa gcgcggagca agaacatcga atgctccttt ggtgattgag atgtattttt
    32161 cgcccatttg gtgaactgtt gtcatcattt ttcgttcaga atcaaaagga agctcgttta
    32221 ctcgtggata tttttcttct aattctgcct ttgtatgata attctcttca acagccctta
    32281 cgatggctac ttctgttgga ttacctttga taacaaggtc gtcatcttct tgctcgaccg
    32341 tcacatctgt acatagtgca gccattttca atatttccat cgcgctgttc gtcattgaat
    32401 cttcgatatt cgttacttca tcttctttgc tccatacccg gcggacgcgc atttcatttt
    32461 gtgtcagcgt tcctgttttg tctgagcaga taacattagc ggttcctagc gtttcaactg
    32521 ctggcaactg tcggatgatc gcatgtttcc tcgccatctt ctgcacaccg tatgcaagtg
    32581 tcaacgtaac aataacagtc aaggtttccg gtacagctgc aacagccaag gaaatcgaag
    32641 tcatgaacat ctcaaggagt ggttctcctt gcagttcacc aatcacaaat actaaagccg
    32701 cagccgctaa agcgatcata ctgattcgtt tgcctagctg attcaatcgg acctgtaaag
    32761 gtgttttttg catagtattt tcgttcaaaa gatccgcgat cttacccatt tctgtagcca
    32821 tgccaatggc agtgacgatc gctttcccac gacctgcagc aaccgtacat cctttaaata
    32881 ccatattaag ttggtcacct aatgactcat cttcttcagc aataaatgct gcatcttttt
    32941 caactgcttc actttctcca gtcaacgccg attcttctac acgtaattgt gatgcttcga
    33001 tcaaacgggc atcggcagaa atagcatcac cggactctaa aacaagaata tcacccacta
    33061 ccagttgctc tgactcaaca gtggtcactt tgccgtctcg tatgacagtt gccgtttgtt
    33121 tattcatatc ttgcaaggat tccagtgctt tttctgcgtt tccttcttgg acaatcgcca
    33181 ataccgagtt gatcacaacg atagcgatga tcagcagtcc ttcaaataaa tccccatgtt
    33241 ctgtagcaaa tgcggtataa aaggaaatca ccgctgctac aagaaggatg atcgttgtaa
    33301 aatcggacaa gcttcgaaag aattttttga tcatcgattc tttcggcgcc tcatcgaatt
    33361 tattcaatcc ctgctttttt cgccgttcat ccacttcttg tgaagacaac ccagtatctg
    33421 gatctgcttc tgttaccttc tttacttctt ctattgtctg cttgtattct tccatcctat
    33481 tcctccttta aaatcttcgt acaaaaaaag gtacttattt tactataaca tccttttttt
    33541 atttacttca aataaagggc ttatcgatta tttgttaaga aagcgtaaaa tttaaagtgc
    33601 ttatatttgc aaacgctacc aaaggatgct attatataga tgtaaggaag gaggatacca
    33661 tcaaatagac agaagaattt ctcgatagaa cagtgtaagc aaacaaagtt gttagaagta
    33721 caggtttcac ccagacatgc gtgtagagta caaacctata aatagtgaat gcgtatggat
    33781 gtttggatca cgaagtatcc gaaacaagaa gtaatgcgat acgtaacaat tgaataacgt
    33841 aaacgtctga aaattgaata ggaacaagag atgtttcaaa tgttgaagat gtaacgaaaa
    33901 caaaattttt gtttaacatg aagaaattca acaccgtgct ttgcatgtat gatggaaagt
    33961 tacaatcatt tctgtttatt tgaatccttc cgaggtacaa gaaagccgag cttatcgaga
    34021 gctcggcttt ttctttgtca taaaatcttt gtaaagcttt tttgttattc aaaactagcg
    34081 gcttttcata accttatttc acgaacatcg catctccaaa actaaagaaa cgatagcgct
    34141 cttcaatcgc atgctggtaa gcagataaag tcaattctct tcctgcaaag gcactaacta
    34201 acatgactaa agtcgatttt ggcaaatgga aattagttga gaaagcttct accactttga
    34261 attcatagcc aggcgtaatg aaaatatcag tccatccgct gtctgctttg atttctccat
    34321 taaatttcgt tccaatcgtt tctaatgttc gaattgaagt tgttccgaca gccacgattt
    34381 tcccgccatt tttacgtact tggttcaatc gctcagccgc ttcttccgtc aaacggtaga
    34441 attcactatg catatggtgt tcttcaattt tatctacgct gacaggtcgg aatgtaccta
    34501 accctacatg tagtgtcaga tagactaatt caacaccctt tgcttcgatc tcatcaagta
    34561 attctttcgt aaaatgcaat ccagctgttg gcgctgcagc agaaccattt tctttcgcat
    34621 agacagtttg atagcgatct gggtcttcca gacgttcttt gatatatgga ggcaatggca
    34681 tttctcccaa tgattcaaga atctctagaa aaattccttc atactcaaag tcaatgatcc
    34741 gtccgccgtg ttccaattct tctttcacta ctgcttgcaa tctgccatca ccaaaagaga
    34801 ttcttgtccc aactttggca cgtttcgcag gtttgatcaa tgtctcccac gtatctcctt
    34861 gtgtattcgt tagaagaagg acttctaaat gcgcaccagt atctggtttt tctccatata
    34921 aacgagcagg cagaactcgt gtattattca taactagcgc atcaccaggc tgtaactctt
    34981 caatgatttc atggaaatgt ttatcttcca tctctccagt tttccgatcc aaaacaagta
    35041 aacgagaatg atcgcggtct tttaatggtg tttgtgcgat taattcttcc ggcaagtcaa
    35101 aatcaaaatc ttctgttgtt aacatatcgt cactcacttt ctatcatgtg cttcatcatt
    35161 ctatcatgtt tttcgttctg ttggcaaaat cagcactttt tcacttctat tttttgtttt
    35221 cctaaataaa aaaactaccc tatttgtatt ttttcattgt tttaggcata ctagtaatag
    35281 atagttcttc attgaaaata gaaaaagagg tggcaccatg attgaatttc aaaatgtgtc
    35341 aaaaatttat aaaggtggaa aagtcgctgt agaggacgtc aatctttcat ttgaaaaggg
    35401 tgagttcatt tgttttatcg gtacgagcgg aagcggaaaa acgacgacga tgcggatgat
    35461 caaccgaatg actgatccaa cacaagggaa aattttgatt gatggaaaat ccatccaaga
    35521 aattgatcca gtggaattgc gacgtcagat cggctatgtt atccaaagta ttggattgat
    35581 gcctcacatg acgatccgtg aaaatatcac tcttgtccaa aaattgttga aagtcagtca
    35641 agaagaacgg aataaaacag ctgaaaaaat gattgatctt gtggaattgc cccgtgagat
    35701 gttagatcgc tacccacatg aattatctgg tggtcagcag cagcgtattg gagtcgttcg
    35761 tgcccttgca gcgaatcaag atatcatctt gatggacgag ccattcggtg cgcttgatcc
    35821 aatcactcgt gattccttgc aagaccttgt caaagattta caagagcgtt tgggaaaaac
    35881 gatcgtattt gtaacccatg acatggatga agcgattaaa ctagcaagcc gtatcgccat
    35941 catgagtgaa ggccgtgtga tccaatttga tacaccagaa aatattttac gtcatcctgc
    36001 aaacgatttc gtagaagagt tgattggaga agatcgccta ctacaagcca agccaaatgc
    36061 gacaagagtc ggcgaagtta tgctaaatac agcaatcacg attacaccgg aaaaatcatt
    36121 acaagaagca atcaaactga tgagggaaaa acgtgtagat acgttgttag ttaccgataa
    36181 ttcgaatgta ttaaaaggat ttatcgatgt agaaacgttg aataaaaaac gaggaaaagt
    36241 atccagtgtc ggcgacattt tgaataaaca tgttttttac gtaaaagaat ccgcctatct
    36301 tcgtgatacc ttgcaacgca ttttaaaacg cgggctgaaa tacgtgccgg tcgttgatga
    36361 acagaacaaa gtcgtaggta tcctgactcg tgcatcatta gtggatatcg tctacgatgt
    36421 gatttgggga gaagatcccg caactactga tgttgcagag tcagctactc ctgaagaatc
    36481 taaggaggtc tgacctatgc agcagttttt tcatcagcac ggttcagaaa ttctctcaaa
    36541 aggttttgaa catctttata tttcaggact tgctttgatc attggtatca tcattgctgt
    36601 accgcttggt gtcttactga cacgttttcc aaaagctgcc acgattgtga ttggcttaac
    36661 cagtgccttg cagacaatcc cttctcttgc tttattggca ttgatgatcc cgctatttgg
    36721 cgtaggtaaa ttacctgcga ttatcgcact ctttatttat tcattattgc cgattttacg
    36781 taatacgtac attgggatga aaaatgtcga ttggaattat cgggatgtgg caaaaggcat
    36841 ggggatgact gagttccaat ccattttttc cgttgaattg cccattgcta tgccaacgat
    36901 catggctggt attcgtttag ctgccgttta cgtgattgca tgggctacac ttgcctctta
    36961 tatcggcgct ggtggtttag gcgatttgat ttttagtgga ctgaataatt accaaccaga
    37021 tctcattttg gcaggtacga tccctgtaac aattttagca ctattggctg attggcttct
    37081 aggtctcttg gaacaccggc tgacaccgat cgcacttaga gaggagaatg aagaatgaaa
    37141 cgtatactaa aaatgaccat ggttcttttc tctcttctta tacttgcgag ttgttccttc
    37201 cctggtcttg ccagcaatac agatgaagac accatttcga tcacaggtgg aatcacttca
    37261 gaagcacaga tcttagcaag tctagttgcc ggaatggttg aacattatac agataaaaat
    37321 accgcaatca tcaacaatct ggctactaca actatcaacc atcaagcgat gatgaacgga
    37381 gatgcttcaa tttcagcggc tcgctacaca ggaacagatt taacgactac attgaacctt
    37441 cctcctgaaa aagatccgaa aaaagcgttt gctaccgtga aagatgaatt tgagaaaaga
    37501 tacggtcaaa cgtggttccc gtcctatagt tttgaaaata cgtatgtctt tcttgttcga
    37561 aaagacaccg cacaaaaata ccatctatct aaagtcagcg atctgaaaaa tgtagcggat
    37621 gaattagtag ctggtgtaga tacttcttgg atcaatcgta aaggagacgg ttatgacggc
    37681 ttccaagaaa cttatggctt ctccttcaat tcgatattac ctatgcagat tggacttgtt
    37741 tatgacgcag tcgaagcagg aaaaatggat attgttcttg gctactctac ggatggacgg
    37801 atcgccagtt atgatctagt catgttggaa gatgatcgtc aattcttccc accatatgat
    37861 gctgctccag ttgtagacaa caagatatta gctgatacgc ctcatttaaa agaagcctta
    37921 aataagttag gtcataccat cagtacggaa aaaatgcagg aattgaacta cgaagcagat
    37981 aataatctag tggaaccatc agtagtagca caacgtttct tacaagaaaa ccactatttt
    38041 gaagggaagt gaagcaattg gataaaatga atgtctttca acaatttttc tattatttcc
    38101 aggaaaatgg ctcctatatc tttgcacaat ttatccgtca cttcctgatt tctatctatg
    38161 gtgtcctctt tgcagcagtt gtcgggattc cggttgggat catgatttct aggagacgta
    38221 aattagcaaa ctgggtgatt cgtttggcga atatcatcca gacgatccct tctctagcca
    38281 tgatctccat tttaatcatt ggattaggcc tgggagtcaa tgtcgtgatt gttactgtat
    38341 ttttgtattc cctcttaccg atcatcaaga atacgtatac cgggatgatc caagtagaca
    38401 aaaacatcct tgatgtcggc aaaggcatgg ggatgactgc tagacaacga ctattcatgg
    38461 tagagttacc actctctgtt tctgttatca tggcaggcat aagaaacgcg ttagttgttg
    38521 ccatcgggat aaccgcgatc ggtgcattcg tcggtgctgg tggtcttgga gatatcatta
    38581 tccgaggaac caatgcaaca gagggcacat cgattatttt agcaggtgca ttgccaactg
    38641 cattaatggc gatcatcacg gactggcttt taggtatttt ggaaagacgg ctagatccag
    38701 ccagccgtac atcacgctga tgttattgta aaaaacaaaa gcttctgcga ccatttgttg
    38761 gtcgcaaagg cttttttagt tctgtcattt ttaaggcaat aatccactct gattcttccc
    38821 tccttatggg taaagagttg cttttcttag ctatttatcc attccgtata ctccaacttc
    38881 atactgccaa cgggttcata tctgcatcca ccgactaagt ttttttctta tccgctaagg
    38941 cattttgcca atccattttc ccatcactcg tcatggaatt cataccacat gatctctaaa
    39001 tactcgtctg cttgttcttt gaattgggca tcaattctta catcctttgg cgtggctttc
    39061 atttaagcct tttccatcag tcctcgattg ttcgatgcct tcagctgcat catcaaaact
    39121 ggtgactgcc gtatcggtaa cttcgatcat cttttataca atcgggaact tgccatcctc
    39181 atccttggct aaaaacatat ctagccattc cagcttctcg ccatgggagg tttgcggttc
    39241 ctaaaaccgg tacacgattc ttatggactg catccgtcgc aactagactt ggcgatacga
    39301 tgatccttca ccagaagagc acccccgtaa accaattgat cgatatattg ccaataagaa
    39361 aagacattgg cgtaaaagat atcgatccct cgtgatgcat tgccgttgat actgctattc
    39421 atggccgaaa gcgccacgac tttaacttct tccgtttgag tgacattcat gttcggcaat
    39481 gtcgctttgg atacccgctc tgctaaagga acgacactgg tattgtattt cgtatctgta
    39541 tcttttccaa aagaccatgc caacgaatcc tctgggaacc aataagacga ctccggttga
    39601 ttgtccattg cttttttcaa catacttttg gctgtttctc gataaacgac atttccaatt
    39661 gtcttcgcct cactttggcg ataatcgatg gtcgttgcca ctgccaacac taacaacgat
    39721 gctccaattt tactgatttt ccttgtcaca cttcttcctc cttgttttac ataaacccgt
    39781 caacattcca ttcctaaact caaacaatga cccttcaaag gtggagatat aggaacaaat
    39841 taaagaaatc atgcaataaa tccaagaagg ttggttttag aacaaaaaaa gagtctcctt
    39901 tttggggaga ctctttgtta actatctaaa aaggttggct cttctcactt cactcataat
    39961 gtgtccaggc agaataaatt tctacaactt tattttcctc atcaactttg taaaccactc
    40021 gatgctgtct attcagcctg cgtgagtatc tgccttcatg tgtaggtctc agcttttcaa
    40081 aagactgcgt gggcatatat ggatcttcct tcaatgtctg aatcacttct tcaaattgtt
    40141 tctttaaatt cgtttgcttg atttttctta aatccacttt cgcagagttt ttgattgcga
    40201 ctgtatagtt actcatcaaa gagcttccca atcaatatca tcgatattcg taaagctact
    40261 gtcgtctttt tcgcggtctc gtactttatc taaagtacct gtttgctcaa gaaaaagggt
    40321 ttcttgaatc gctctccagt cttccagccc aatcaataca gcattgtttc cactacgatc
    40381 actaataatc tcgatttcag tatgattttc atttacctct ttcaataact gataaaaatt
    40441 tttacgagca gtgctaggat tcatcgttgt attagccatt gtctctcctc ctttttctga
    40501 tataattata gcgtacttga ttaagtacgt caaaaaaata caataaagca cttaaattca
    40561 gcaaactttt cctctttttt acatagagaa aaagacccct ttttagggag gatctttgtc
    40621 cacgctcact tcttactcct ttacgactag cggtagccaa acgtaaagta attggtaaca
    40681 acgtatcatc tttagcaaag gtcgaatcca aagcctgtga gtctatgcaa caacaccaat
    40741 acacgaaggt tcctcgttgc agtgtgactt gattgatatc ttgaataacc tgaggatacg
    40801 cacgaaagat ttgtaacggt tattcaaaac tgattttggc cgtcaagtct tctactgcct
    40861 caaaaatgat tgcctctttc gtctcctgtc attcatgttc actatcgatt tgatacttgc
    40921 ttagaccgtt gggtttgtga atcaccaatt aaaaatggca ctgagttatg gtaacaaaaa
    40981 atggtcactt gacgaggctg agattcctac ttatgaagac acatggcact taagagtcga
    41041 ggtaaaggaa gaagctgaaa atataggagg ctgtacatta tttggcaaag gattcggaaa
    41101 ttatgatcaa gacatcaaaa tcaaaatgtt ttacagctaa ataggaagaa taataacatt
    41161 tcatacgctg ttcccatctt taatggctta tagagggcac ctttagctga gaataagaca
    41221 aaacagaccc tctagcttca attaagagct agagggtctg tttttgattt taaaacttgg
    41281 tttatttctt caaatggtag ctataggtgc tgacaacgat attttcacgc caagacaatc
    41341 gcaatcttaa ccgcaaactt gtttggttat ggagaatatt ctgccagcgt ctgtttggta
    41401 caaattgagg aatcaacaca gtagttgtat agttttgttt cgctgcattt ttacttacga
    41461 tatctacata acggataatt ggattttcaa tcgagcgata agaagagtgg acgattgaaa
    41521 aacggacatc tgggaaatgc tttttaaatt ctgcttcgat ttctttttct ttttcgacat
    41581 cttcgtctag cgaaacatgc atcgcaacga cataatcacc gatcgaacgt gcgtaattca
    41641 acgcaccgat atttacccgt gtcacattcc cgactaatac aagaacggta ttcccgtcat
    41701 aatcatgtag ttttgcatca ttttctaaac gaagctgttc tgctactttt ttataatgat
    41761 catggatctt ataaaaaatg aacataacga ttggcataat gatgaagaaa ggccaaatgt
    41821 cccctaaccg atagacaaac aaaatcgcaa tgattgcata tgagatgaac gcacccgtaa
    41881 tattggcaat cgatttgctt aaccatcttt ttccttcttt tttccatttg acgaccatcc
    41941 ctgtttgaga taaagcaaat ggaatgaaca cgccgataga gtataacgga atcaagcgtt
    42001 ctgttgagcc atggaaaata aacagcaata cgatcgaacc tagcgctagt gtgataatcc
    42061 cattggaata gcctagtcga tccccacgat cttgatacat gtgaggcatg aatttatctt
    42121 ttgccagatt gtatgcaaga actggaaaag cagaaaaacc ggtgttagca gcaactgcta
    42181 aaatcagcgc tgtagcaaat tgaaggacgt aatacaaaat accatgtcca aagacagctt
    42241 taccaatttg agacaaaaca gtgacttctt tttcaggaac gatcccatac caatagttga
    42301 taaatgtgat ccccacgaag aagaaaccta agatcaatgc catcatcgtc aatgtagcag
    42361 cggcattttt tgctcttggc ttcttaaaga atggtacagc attactaatc gcctccaccc
    42421 cagttaaaga ggaagaacca gaagaaaatg ctcgtaaaat caaagcgata gaaacacccg
    42481 gcacagccgc tcctggaagt gcagtagctt gcaatggcac agcacctgtc acgatattat
    42541 ataaaccaac aacgatcagt aacgagatca caaggataaa tgtgtacacc ggaaataata
    42601 ggaacgatgc agattccctc agcccacgaa gattcagcat catcagtagc aaaacgattg
    42661 tcacagaaat agcgacttga tgaccgtaga gtgctggcac cgctgatgtg atcgcttcag
    42721 cacctgctga gacagatact gctactgtca acatgtagtc gatcaataaa gagccaccgg
    42781 aaatcaagcc agcattcttt ccaagattct cactgctgac gacatatgct cccccgccat
    42841 gaggataagc atggatgatt tgacgatatg atagcgttaa tgaaatcaat aaaataataa
    42901 caaaagcggc aatgggtagc gaataccaga tggctgcagc agacaaagcg actaaaacga
    42961 caacgatttg ttccgtacca tatgcaatag atgataacgc atcagacgac aataatgcca
    43021 aagcagcaaa tcgggttaat ttgtgctcat cattttcagc agattttaaa ggcttaccta
    43081 ctaacaacct ttttaagtaa tccaatttct aaactccttt acacacaatt ttacttcaag
    43141 ttttatagtc gaaaatttat agtaaacaag atgttatagt acgctcactg accttaaaag
    43201 tcaataaact ttaagaaaat aattggaaaa aaacagaaaa atatcaagcg ctttttcaaa
    43261 cgctttaata cagcaaaaat ctaacatttt tttatgggct ttttctttga attctataat
    43321 aaaaggatag aacttgactt agaaaatcaa gttctatcct ttttgatatc ttaacttttg
    43381 gaagagagat gtaaggctgc tttctaccca tcattcttac tactcgaagc atagcgcagt
    43441 tgtcacaacc tcttgataaa cggtgtttaa aagcttgcat ctgcggtaat aagcccaacc
    43501 aaattaaaaa cataagaagc tgtttttatt tggtcgttct tattacttga tgcagagcag
    43561 cttttttaca acctcttggt acacggtgtt caatcagctg accctcggta ttagctcaaa
    43621 aaaacggcaa aatatgagga actatttttc catttttttc tctaatactc aggtcaaaac
    43681 actgatttca caacctcttt ttttaatgaa aaatatggct ctcatgcgcc gcttctttga
    43741 atttcgtcat tttttcttcg atgaacggat agattgccgt tcctacgatt ccaaagacaa
    43801 caaacaaacc gatcatgatc cagattgctt tcgtagcatt tggccaataa attcctcctg
    43861 cagattcccg taacaagttt accgcatgag taaatggcaa gaacggattg atcatttgga
    43921 agaatttccc tgagacttgg atcggatagt tcccgcctcc gccagagatc gacagtacta
    43981 agatgataat agcgatccct ttaccgacat tatcgaataa tgcagccaag acatagacga
    44041 tcatcatgaa cgctaaggcg atcaagacag caaatgacac gctgtagacc ggattttgga
    44101 catctactcc tagtaagaaa atattcccta atgtaacgat caagctttgc gcaatcgcca
    44161 ttgtcaaaaa tgtcagcata cgagcaacga attgttctcg tttcgtaaaa ttgtttcggt
    44221 ctttctttcc taaatagtat tctgtcgtag caacacttga aagaagaaca gcgccgaccc
    44281 acagacatag tgcagtataa aacggggtgc tagctgaacc gttatttgcg attggataaa
    44341 gcgcattggt ttgtagctca acaggttgtg taaagaagtc actttctttt gttgcgtcta
    44401 gtttcaataa tttgatgact tcgcctagat cgacttcttg ttcgcctttt cggatcgcgt
    44461 ctgctgcttt ttggatacca gctttgatat ttggccagtc attgttgatc aagtccgcag
    44521 cagcgttcac agctgtttcg acttctggca ttttttcatt tactgttgtc aatgtaccgg
    44581 tgatttcatt ttttagtgct ggccaatcat tttggataaa atcagcggcc aatcctaatt
    44641 tttgctgaat gatcggtaat tcattgttgt ataggtctgc cccttcattg atgccattga
    44701 cgatggtatc catatttcca ttcagcattg tattggcatc atggatttct tgtccaattg
    44761 caggcatttc tttttgatat ttttctaaaa ttgccactgc attcgttaca gttgctttcg
    44821 ttgagctgag aagcgaatca aagtcgatct ggtccgcttt gctcagtgcg ccttgtgcag
    44881 tcccaatcgt atcgatgact tggtctagta gagaagtgac agtggaactg atttgacctg
    44941 tattgatacc agcaatgatg ccattgatat tattaacaac ggcatcaatt tgatctagca
    45001 attcatcaat agccttcgtg tcttcatttt gaacagctgt gttcaaagca gataggcggt
    45061 tttgcagatt gcttagcaag ggttttaatt gatttagctg atcaataatt ggctgtaatt
    45121 tcgtatttcc agcacttgtt tgcaatttag tcaggaactc gaccagttta tcgattgcat
    45181 tttgttgttg agatattccg tcaataaagc cgttcaatac attgctgatg tttttctttt
    45241 catcatcagt cagcttacca tcttcaatag ctgttcttac agtatctatt aacgaacctg
    45301 tcgttgttgc cacttgtcca attgacttca atgtaacatc tacactgttt gtgatgcttg
    45361 gtatcgcatc tttcagctga gaggcacctg attttgtttg gtttgctaga tcacttgcct
    45421 gattttctaa atttttcaca tctggcaaga tatcttgtac ttgttggatg atcgtcaagc
    45481 cttgttttgc ttcatcgatt ccttggttca ttgtctcttg gatcgaagca aaatcgccgt
    45541 cgatctctgc tagttgctta ccggcatttt ggatctctgg gattttttct tgcaaatcaa
    45601 ggatgatttt tgcttgatct ttcaactcag gcattttgtc attcaatgca acgacttttt
    45661 cacccatctc atctacttta gggatgtaat ccacgaattc atttgcttta ttcagttttt
    45721 ccttgatttc aggtagttga ctttgtaatt cgaggacttg ttttgtgtag ccgtcgatcg
    45781 tgtccaaatt ttcatctgtt gataaaatca tatctttaac tttattgata cttactaggt
    45841 tcgtatcgat gtcgtaacct atctcattga atactttcaa taaagtcgaa ctggctgttt
    45901 tgataaattc atttgtgatt tgcgattgga tcgaacttgc tcctttatcg gtgattttcg
    45961 gtgcaatcgc attgattttt tcatttaccg tatattcgat ttttggtttc ttgatatcac
    46021 cactagtaaa actcagcaga tcttctgaga aatcctttgg caagtaaata cctgcgtaat
    46081 actttccgga acggactccg tcttctaatt gttcttttga atcaacgaat tgccatccta
    46141 attgtttatt tttatgaagc gattcgatga cttgctcccc aatggccact tctttacctt
    46201 gaaactctgc tggcttgtct gcactgtaaa cagcaattgg cagctctcca gtatttccat
    46261 atggatccca taatgctttg atgttaaacc acgcatatag cgaagggatg atcatgatcg
    46321 cgatgatcag aagtgttgca atcgggtttt tgaaaatccg tttccagtca agcaaatata
    46381 atcgaagcgt atttttgatt gactgcatag ttctccccct tcttcttttt tcagctgaat
    46441 tactttacca tgtatttcat acttttttca atcggctttt ttcaaaaaag tcgaattttt
    46501 ggacatatca cggcaattgt ctcattgtaa tctcttacat attgagaaat aattaaggat
    46561 tacagccttt actttatcat gaaataaaag ggaaagataa ttttatgcca aagtttttat
    46621 ctattatacg tatacgcaaa aaagctaaaa cattttttgt ctttgcccct acaaaaatgt
    46681 ttcagctttt ttcgtttttt attcctgtat ttcttatttt tcgaatgaca ggatgatttt
    46741 cccagcagga ggatgtcctt caactagctg atgtgcttgg atgacatgct taagtgaagc
    46801 agacagattc attgaaataa aaacatgaaa ggctcctttt tggtaggctt tgattatgcc
    46861 agcaaaggct tctgcatcta gatattcctt tcttggaaca aacgattcat aaaagccttc
    46921 ctttttttgc cgcaaatcta gatctgggag gtcatttaat gcaacatacc tgccgcctgg
    46981 tttcatgatc tggatgccgg tttctccttt gatacttcct ttcgtcgcat cgattacaag
    47041 gtctgcctga tcagcaaatt gcagacctgg atttgtttta tcataagcag caaatgctga
    47101 agctcctaat ttttttacct tttcctcatt tttactggat gcactggtta agattcggat
    47161 tcccttttca tggagaagct ggatcagact tgacccgacc gcacctgatg ctcctagtat
    47221 cattacagta tctgtcggct ggatttccgt caaatggttg attagattat aagccgtaat
    47281 tcctggcgtg accattccgg ctgcttcttc ccaactcatt ttatctggaa tttttgctac
    47341 ttttgctgat ggcaacacga cttcttctcc gtaagtcccg ccgactgcat ggacagctac
    47401 tcgatcacct acatgaacag ttgtgacatc tgaaccaatc tctgtcacaa taccagcgcc
    47461 atcattacct ggtacataag ggaatttcaa tgttcgtatc tctttcattt ctcctaaacg
    47521 caaggcaaca tcataaggat tcacactgaa tgcttttatt tctacacgaa catgtccaga
    47581 accgacttct ctgggatgag catcgatttc ttcaaaaaca tcttgtgctt ctccgtaacc
    47641 gttaattgca aatcttcgca tggtcaccat ctcccttttt ctttaagttt acgtttatgc
    47701 ctttattttg acaaaaaaaa tgtcttttcg ttttggcgca tgattcaaaa caaaatagtt
    47761 gtcttttttc gcgtaacgtg ttaaattatt tatctaaaag cgtaaagatt acgatttacg
    47821 ctaataaaaa ataattggag gaatttgttg atggctaaaa aatcaaagat tgccaaagca
    47881 aaaaaacagc aagaaacgat tgaacgctat gctgctttac gccaggaatt aaaagcgcaa
    47941 aaagattacg aaggcttgcg taaacttcct ttagatgctc gtccagaacg attgaaaaat
    48001 cgtgatttaa tcgatggacg ccctcgcgga tacatgcgta aattcgggat gtcccgtata
    48061 aaattccgtg aattagcaca taaaggtttg attccaggag taaaaaaagc cagctggtaa
    48121 tccaagtctg gctgtaatgt aaaggaggag ggcgcatgga ccactccttg gatcatcaaa
    48181 aaagatacgt tcgcttcttc tagcagaaat gctggtacta gaagcagacg tatctttttt
    48241 tacgcacagc tggtcctttt ttatatgtaa aggctgcatg gttacttcgc tatatttatt
    48301 tagtaacagg agtcagtgct tcgataccca cactaatttt caatgctcgg tcaacttctg
    48361 ccataatggg tcgatccaaa tggcagacct tttctttcaa tcttgattta tcgatcgttc
    48421 tgatctgctc caacaggata acggagtctc tttctattcc tgttccagta gctttgatcc
    48481 caatgtgagt cggaagctta ggtttagcca tttttgccgt tatggccgca acaatgatcg
    48541 tcgggctaaa atggttacct agattatttt gaataaccaa aactgggcgt gttccgcctt
    48601 gctccgatcc cacgactgga gaaagatcag caaaataaat atctcctctt tttaccatag
    48661 gatcctcctt attcttgata ttctcttggt atccgctgtc ctaatccgca agccacttca
    48721 tagtggatcg tacctaattg atctgctata tcctgcatag tgatttcttt gtctgcattt
    48781 ttgccgataa gcgtcacttt tgttccgaca ggaaattctt gaggcaatcg gatcatcagt
    48841 tgatccatac atacacgccc aatcgtttca cagtagttcc cttctaccaa tagagaaaaa
    48901 ccctgcatct ttcgtggcca gccatctgca taaccgatcg gtatcgttcc gatccactct
    48961 gcttcaggag tgatatatgt ttctccatag ccgattcctt cacctgctgg gagttttttg
    49021 acttgaatca attcagaaac cagctcaagt gctggctgca atggataaac ttccggtagt
    49081 gcgtgcccag agggattaag tccgtacatt gctactccat aacgaatcat attgccgatt
    49141 gtttcatcat gccataaagc cgttgcacta ttgctgacat gtacatatcg aggaagttcg
    49201 ggaagttttt tcagcacttc tataaagcgt tctttttgca gattccaata tgtatcatct
    49261 gcctgatctg ctgtagaaaa atgggtaaat aaaccttccc attctaatac acgactttct
    49321 ttgatgaatg cagctgcctc ttttacttct tcaggtgtac aaaatccgat acgacccatc
    49381 ccagtgtctg cttttagatg aatcgaaaga ggcgtctcta gcttactttt ctctaaaaca
    49441 gcagctgctg cttgcaacca ttcccgtgta cctgcagtta ctgataaatc atgagcaaca
    49501 gctacagtca gtgcgtcgta aggaaccata ttcaagataa ggatgggctg agtaaatcca
    49561 gcttctctca actcgactcc ttcatctaaa tttgaaacac aaaagcccga agctccacct
    49621 gcaactgctg cctcagctgt ttcaatagct ccatgaccgt atccgttcgc ttttactact
    49681 gcaaacaatt cttttccttg cggaagtctt gcaacttcat ttgctacatt ttcagttata
    49741 gcttttttat gtatcactgc tttcgttggg cggtgccaag cgacaaccat cgtttttcct
    49801 tctttctagt cttccaaaat aatttgtgca aaagctgttt catttgtatg agtaatactg
    49861 acccatacat tgccttcatg aggtgaacgc gtcacaacag gctgaccatt ttcatttttt
    49921 aaaatctcga catcttgaaa ggtaactttt ccgatcccag ttccccaagc tttagagaaa
    49981 gcctctttgc aagcgtaacg cccgcctaaa aattctactt ggcgatgaaa aggcaaggac
    50041 tggaatagtt tcatttcatc agaggtcaag attcgtgcaa tgaattttgg tttttcttcg
    50101 atcagccgag tgatcctagg aagttcgact gcatcgatcc caattccttt tatcatatgc
    50161 ttttctcttt tcaataaatt tagaacactc tcattctacc aaattataac cattttgcca
    50221 caaataagtc cacaaataac aaaaatgaaa gtttctctta aaaaagagaa actttcattt
    50281 tatgtttaat cgttatttga acggattaca aatccgcgtt ttttatcatt gtgacgttta
    50341 ccgccatctt tttggctgtt attatttttg ctataaccgc cttttgattt gtttccacga
    50401 taattaccgc cgtcacgacg attacggttg tttccgccac cacgtttgcc atttttattg
    50461 tatccttttt tctgcattgg caatgggcgt tctggtgtga tcttcactgg cactgcatca
    50521 gctggatctt ttgctgtcgt ttttagcaaa agtgcaacta aatcttgtgc agaatattct
    50581 tctaataatt tatctgctgt ttgcaggtat ttatctagac cattttctgc aagttttgtt
    50641 tcgatttgtt caactgcagc acctaattga cctttgaatg cttctttttc ggttggtgga
    50701 cgtaaagtcg tcatccgttt tttcgtcaag ttttcaatca catgcaagta gtccatttca
    50761 tttggtgtaa cgaaagtcac agacataccg ccttttccgg cacggcctgt acgtccgata
    50821 cggtgaacgt agctttctgg atcttgaggg atatcatagt tgtacacatg agtgacacct
    50881 gaaatatcta atccacgtgc agcaacgtct gtcgctacaa ggatatctaa atgaccgctc
    50941 ttaaatgaac gcaatacgct cattcgtttt tgttgagaca aatcgccatg gattccttct
    51001 gcacggtaac cgcgtgcttc taatccgcgt gccaattcgt ctacacggcg ttttgtacgt
    51061 ccgaagacga ttgttaattc aggtgtttga acatcaaaca agcgagtcat gatatcgaat
    51121 ttttcgtatt cttttgcgcg gacatagtat tgatcgatca agtcagctgt catttctttc
    51181 gctttgatct tcacatgatg tggatttttc atgaatttaa cgccaatatt cttgatagct
    51241 ggcggcatcg ttgctgagaa aagtaatgtt tgtctttgat caggaacttg tgaaatgatt
    51301 ttttcgatat cttccaagaa gcccatattc aacatttcat ctgcttcatc caaaactaat
    51361 gtttgaacgg ttccaagttt caatgtgtgg cggttaatgt ggtcaagcat acgtcctggt
    51421 gtacccacaa cgatatgtgg acgatctttc aagccacgga tctgacggcc aatatctgct
    51481 ccgccgtaaa cggcttgtac acgtactttt ttgtcgcgcc ctaaacgata taattcttct
    51541 tgtgtttgga tagctaattc acgtgttggt gcgataacta aaccttgtaa ttcgtgacga
    51601 tctggatcga ttttttctaa cattggtaat ccaaatgcag ctgttttccc tgtacctgtc
    51661 tgtgcttgtc caataacgtc ttttccagct aatgctagtg gaatcgtttc tgcttggata
    51721 ggcgttgctt cttcaaaacc agcgcgttcg actgatttca acaattctgg tgataattct
    51781 agttctttaa atttcaaatg aatcctccta tatggttgtt aactgaggat gtagcttttt
    51841 tgctaccccg tcgatttgtt cggtcaaagt gtattgtttc tttttgacag tggccacgca
    51901 cgtttttatc cattcttagc ccctgtggca ccaccgaaaa atcactttgc gaagtttagg
    51961 cattagaaaa gccaaacggg tcgcgttcag ctttatcatt attatgacat ctggcttcaa
    52021 caagttgttt ctatggcaaa accagtccaa tttctcttca tctttcggca ataggttatt
    52081 ctaacatagt ttttcgtttt ctgcaagttt acagattttt atttcatttg ctgaagttgt
    52141 tctaccactt caaataagcc catgctgttg ctgcctttta gcataatact atctgttgct
    52201 tcaagatttt gtttgatagc atcaatcaat ttattctttt ctttttcaaa atagaacaca
    52261 ggtagttggg ggtatttttg gttcaattct tcttttagtg catgcatttg ttccccataa
    52321 aggaaaatca tggcgaattt ctcaggatcg atatgttcac tcatttgcgc atgcatattt
    52381 aaagactctt tccctaattc cagcatatct gctaacacag cgattctgcg tccttttgtt
    52441 ggcagatttt taaaagtatc taataccagt cccatggctg ttggatttgc attgtagaca
    52501 tcgctcaaaa tagctgcacc atttcctgcc gttagccatt cggttctgtt ttgagtgata
    52561 gaaacatggg cgagcccttg gcgtatctca ggtatggaca gtccaaagaa gcgcccgatc
    52621 gtatacgcaa tcaaagcatt cgttacatta taacttccag gcagcgggat cgtaaatgtt
    52681 tctccttcta ctacaaaatc ggtttgtgct tttccttttg ctgtcacttc cgcatgaaca
    52741 tcgccatttc caataccgaa tgttgtgact gtttgtacta atgttgccgt taaaggtgtc
    52801 agcaacggtt catctgcggg cacaacgagt aatccgtctg atacaagtcc gtcagtgatt
    52861 tccattttgg ctttagcgat tccttctcgc gacccaagat tttctatgtg tgcttcaccg
    52921 atcattgtga ttgctgcagc ttttggttga gccataagtg aaagttccgt gatttcattg
    52981 gcgtgatcca tgcccatttc caaaacgatc ttttctgtat cttggggcat gtgcaaaatc
    53041 gtgtaaggaa gaccaatatt attgttgtaa tttccttgcg tcttatatgt tttgtatttt
    53101 tcagacaaga cagacgcggt tagatctttc gtggtggttt taccattgct tcctgtaatt
    53161 gcaacgactt ctgcgcctac tttttccaag taataggttg ccaattgctg catggctttc
    53221 aaaggatctg ctacaggaat aactggaaaa ttttttggag cttgttcaac aggcaagctc
    53281 caaaatgaag cagatgctcc gtttgtgatc gcttgctgga taaattcatg tccatctcgc
    53341 gtaccagcaa gaggaacgaa taaacttcct tcggttatct tccggctgtc aaattcgaca
    53401 ttggtaatct ttgtcagtgc agagcattcg ctgcccacgg cttgtgcaat ttcttgtatt
    53461 gttagattca ttgagatctc tcctaaaaaa ggttgtgaaa aaagcgtttg gcctaaacgg
    53521 cgggttcgca tgactctcac ttccaagtaa atttctctat tcgtatttag aagctagaat
    53581 gaacgcggtt acgcaatcac tccagcctct tctttatttc tctttattca tttttttcaa
    53641 aaaaagattg acgctgatgg tatcgattca ttcctaactg gatcagttct tcaatcaaat
    53701 caccgtattt caagcccata ttttcccata agagtgggta catactgaac tccgtaaatc
    53761 ctggcataga gtttaattca ttcaggaata attcattttt atttgtcaaa aagaaatcgc
    53821 accggctcaa tccgcttcca cctaacatcg tgtaagctaa cttcgcgtac tcttgcgctt
    53881 tttgataaac ttcttctggc acttcggctg gaatctgcat ttcgatttta ttattgatat
    53941 attttgcttc ataatcatag aatgctacgt cttttacgac ttcaccaggc aaagtcgtcc
    54001 gaacgtcttc atttcctaat acagcaactt cgatttcgcg cgcttcaatt ccttgttcaa
    54061 cgattgctcg agaatcatac tgataggctg ttgctaaagc attttgcagc tcttctcggt
    54121 tttctgcttt tgtaatgccg acactagaac ccatattcgc cggtttgaca aacatcggat
    54181 aaagcaaaga accttcacat tgatcaaata cttttttagg attttctttc cattgattct
    54241 taagtactgg tacataagga acttgcggca caccagcagc ttgtaaaata tacttggtca
    54301 tgattttatc catggcacat gcactggtca atacgcctgc gccgacataa ggcatattca
    54361 atgtctctaa gaagccttgg atcgttccat cttccccgtt tggcccatgt aaaactggaa
    54421 aaacgatggc tccttcttct ttgatttcgc ccggattgat cacttttcct gtaaagcctt
    54481 cctctgtctg tccacttggg tcccatgaaa gatgcaagac atctttgctg gcaggttttt
    54541 ctgttagtaa tggacctttg acccattgtc cttcttttgt aataaatacg agttgaactt
    54601 ggtaataatt ataataaatg gcatttaaaa ctgaaaatgc ggaaagaatg gacacttcat
    54661 gctctgcgct gcgtccgcca tatagtaaag taatcttcaa aagattgtcc tcctgtattc
    54721 taaatcgttc agtgatttac tctcatattt tagcacaaaa caaaaataaa cgaataactt
    54781 atttataaaa aaacgagcca gcttctaaaa gaacgagcgt catttatttt ttgtcagcta
    54841 gattatttgt cccgtttttt ataaaaagtt atgataaaga aaaggaggga tgatgatggc
    54901 gcaaacaaat ccacgcagcc gcttggcaga aatcattcga gtgttcatca attacaatgt
    54961 agtgccaaac tttgtacagc aaaaaaatcc cgaacaggta aaaaaagcct ttgaagaact
    55021 aggccccacc ttcatcaaga taggacagat gctttctgtt cgagaagatt tgctttcttc
    55081 tgccttcact caaacattca aaacattaca agatagtgta cctagcgaca cattttcaac
    55141 ggtcaaaaag acgatcgaaa cagaactttc tctttcatta tcagatatat tcgatgattt
    55201 ttcaaaatcg ccttttgcct ctgcttctat ggggcaggct catcgtgcca agctgaagaa
    55261 cggtgacgcg gtagtcgtta agatccaaca ccctaacatc gccgaagaga tccgacttga
    55321 tcttcaacta tttgaacgag cgatcccttt gatcaaatac attcctgaaa ctagcgtagt
    55381 tgatctaaaa ggggtactcc aagaagtaaa acgttccttg atcaatgaaa tggacttctt
    55441 aaaagaaagt caaaatggcg aacaattcta tcaaaagaac aatggctgga aagagatccg
    55501 cagccccaag atttatgacg ctttttgttc gaaaaaagtg atcgtgatgg aagaaatgtc
    55561 tggtaaaaac ttgaatcatt taatgaacgc cgaaaacaaa actgaaactt ttatcactgg
    55621 aattcaaaac aagcagctga aacaagaagt tgcaaagctt ttagttgaga actttatgaa
    55681 acaggtgttt gatgatggtt ttttccatgc tgatcctcat ccagggaatc tgctattcca
    55741 tgtactaaca aaagaagaac agacacaagc tagccgaaaa actgaaacag tccatgaaaa
    55801 agagttcggt tcctttgctt ttcgagcctc cacttctgca gaagaccctg ttgcgccata
    55861 taccatcaac tacatcgatt ttggtatgat ggggcatctt tctgcaggac ttaggcaaaa
    55921 actcacacaa gccgttttag cgctgtatac gaaagatgct tatcgaatcg aaaaagcggt
    55981 ccttcgactt tgtcagcaag aaggctcgtt tgatgaaagt cggttccacc aagaactgac
    56041 aagttttctt gaacagtatt atgattctcc tattgatgaa atcaacttgc aagaagtatt
    56101 cacacatgtc gtgacgattt gtcatcaaaa taatctgcag ttcgatcgtg atatcacgct
    56161 tttgctgaaa gcatttggga cattagaagg ggtgatccgc gtcctcgatc ctgaagtttc
    56221 cctcatggaa gtcgcttcgc cttttgctca acattatttt ctcactcatc tggatgtaga
    56281 agatacattg aaacaaagcg gattagactt attggaaggg atgaaggctg cgcctaagat
    56341 ccctcaacag ctccaccatc tattagaaat gtggacttcg ggacaaggaa aagtcaattt
    56401 ggaattgaaa aagcaagaca aattattgag caggatcgaa agtatgatca atcggcttgt
    56461 tttcggtatg attttagctg cattgatcgt agggtcctcc ctgcttgtcc aagcagcacc
    56521 tgtagaaaac gcagaggtcg tttccttatt agggatattt acgtatgcta ttgccgcatt
    56581 cgtcattatt tttttggcaa tcgatgcatt gatccaaatg tataagaaga ggaaaaagta
    56641 gaaaaaggtt gtgacagaaa gaatcagctt caagaaataa gaaggggttt tacataaatt
    56701 gtttctcaaa tttttgtgaa aattcggctt atttttcgaa gaggctactt ctgaaacacc
    56761 gagaataaaa aatgtgaaaa agctgttctg cacccagtaa taagaacaat caaaaaggga
    56821 acatggaaca catttatcct gtttccctgt tccctttttg atgtattcga gcataaataa
    56881 aataccttcg ttacctacta tcttctgtac ttggtaaacc ttagtcaatt gcctttttta
    56941 acgcttgacg aaaagacata tgatctttct ccgtaaaggg ttttggaccc tttgtcctta
    57001 tgccagcttt tcttaacttt ccactatgat aacgttgttc tagaagcgtg ccgatattcg
    57061 cttcgtcaaa ttcttttcca gagtgatgga acactcgcac acccttcggc aatacaagtg
    57121 aagctaaacc ataatcttgc gtgatcaaaa agtcattctt cttaaccata ccgacgattt
    57181 tataatccgc actatcagct ccgcggtcta cgtagacgat ttggacatga ttagcgtatt
    57241 ctttggtcgt ataatggtcc acactggtca cgataaccac gtcaagacca taggattctc
    57301 ctaattttat gacatcttct tttactggag aaccatctcc atcaatgaat agcctcattc
    57361 tttttcgcct tcttccataa atgataaaca gttactaatc cgctcagtaa aagaccaaca
    57421 ttgatccatt ttgccaagat aagcagttgt acactttcta tcggcactga ttcatggttc
    57481 agcgtgatca ataatagatg gattggctct cccatttctt ttgctaaaac aaacaaaagc
    57541 gtcatcaata aaaactggtg gatcacactc cctttatttt gagtagtata cttgacaacg
    57601 atccggcgat ccattggaat agctgtatca gtttgataat attccttatt tagccagctg
    57661 cctgttacta cgtgccagaa actaagcaga aagacaccta tcgcaaaacc atttggaatc
    57721 aagaggacca ataaagaaga actcagtcca agcaattgca tagccaatgg agaccatgtt
    57781 aaacgtttca aaatccatgg tccgactatt tttgctgcaa tcaagcctgc tgcataaggc
    57841 aaatacataa aaatcgaaag tttttctaat ccgtacagta tgccaacata aaaactgcta
    57901 aataaaaaaa ggaaattccc tagcattcca tttagaaaag taaacagatt tagccagctt
    57961 ggcaacttcc attccttctt ctggtgtcct aaaacaaaca cgaacaagat gaacagcagc
    58021 aagaaaccat aaatagcaag atcaaaaagc ctttcatcga ataacaatcg ggcatttctc
    58081 aaaagaaaca ataagccaaa gaatatcaaa aataaaacaa gctctcgcgt agctaccaga
    58141 tttcttttga tgtctttgaa atccagttcg taacgcggat aatgactaac cgtatggtag
    58201 gccatgacgt aaaaaagtgt ataaatagta aagcttgctg caatctgagt cggtgcaggc
    58261 aactcaatcg cttgatacag gagaaatagc cagatagctg caaataagta ctttttccca
    58321 tccatattga taaaaccttg cgtcttttcg tgaaaattga ccgtggtatt agcaggtaca
    58381 agccatgctg cactcagccc tagcaaaaca cttgaaaaat aaaataaagg aaaataaaat
    58441 acgcctaata ttccaagcaa tgaaccagca ccaccaatca gcagagcgat catcagcaac
    58501 gtgtaactat ctagaccaaa tcgtagactg ttgactaaaa atagtccagt catccgaaag
    58561 gcataaaaca gcgtaaatgg caaaatcgtc agccaattat gcgagtctgt tagtcccaaa
    58621 aataaaagat aaggaataaa aataaccgtg ttacttaaga taaagggcgt tgtttgtgta
    58681 aaattacgcc caaatcgttt gagtttttcg aacatgacaa tggctcctta aacagtctta
    58741 taactaatct gctcttcgat tttagcatta aaggggaaat aaaccaattt tacagaatat
    58801 attcttgtaa aaaaaaatac agaaagaagc ataagcttct ttctgcattt ttacggtact
    58861 tgattactca agcagacaag cccttctcca caaaagtctt gcccgacacc cctgcatccg
    58921 gcaggtgata aagatagaaa tcaattatcc tgtgcctcca aaatcaatta ggagctctgt
    58981 aaaccagagt ccttctcgac ccgtcgcata aatttacttt aacacagttc ctatgtttat
    59041 gcgagagctt ttttcatttt ataatcgacc atttgaaatc cttgaccgat atctgaaata
    59101 agttcttctg taatctcaaa tccttcatgc ttataaacct cgattgcttg atgattcccc
    59161 tgattcactc gcaaaaagat ctctctttcc tttgtttgcg cagcaagttc ttcataccag
    59221 cgaaaaactg aagaagtcaa tccttgacca cgtactttat ttagcaaata taatttactt
    59281 aaataaattt ttccatttat ctcttcataa gcagtgtaac cgacagtctt ttcttcgctt
    59341 tttaataaaa agtaggacac accttctgtc agttcttttt gaatctgttt ttttgactga
    59401 taagttttca gcatgtaatc cacttgtttt tgaccaatga tcggcacaaa aacttccggc
    59461 catacttctt gtatgactgc catcagctca tccagttcat tttctgttac ttttattttt
    59521 tccatacacc cacgtccttt tttacattat aaatgacctt gcgcttgatg gaataaaaag
    59581 agcatgatca tgctatacac acccaccgac cacggtttca agaaaatcac aatctgcata
    59641 aattccctaa aagataattt cgtcaaacca gctactagac agatcaagtc gtctggcgca
    59701 aagggaagca gcaaggtaac aactagtact tttttaatat ctttcgtttt tcttgtcagc
    59761 aattcatcaa atttctgata cttttttggc gataaaataa gctggacaaa ggctacgcca
    59821 tagtaacgga caagcaaaaa tccgattgct tccccaatca ctaggccaat acaactatat
    59881 aaaagcccaa atccattgcc aaacatcagc atgcccgcta ctgaggagat ccctcccggc
    59941 aaaataggaa tgatcacttg gatcacttgc aacagaacaa atatcatcac tgcatacttg
    60001 ccaaattgct ggataaacgc ttgcaaagac gtgctcgatc gaaaaatccc atgatgataa
    60061 gcataaagaa tcaacacaaa gaaaaaaaac agaccaataa tgggcatgat ctgaagacat
    60121 ttttgtaaaa ctgatccttt tttagacatc tatattcccc acatcttttt tcatttatta
    60181 aaactgcctc ct
//
LOCUS       DACDKM010000007        50570 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20834_2016
            SAMN12640370-rid9891663.denovo.007, whole genome shotgun sequence.
ACCESSION   DACDKM010000007 DACDKM010000000
VERSION     DACDKM010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640370
            Sequence Read Archive: SRR10040891
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 50570)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 50570)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742835.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742835.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:04:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,012
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 29 of 75
            Pseudo Genes (incomplete)         :: 33 of 75
            Pseudo Genes (internal stop)      :: 19 of 75
            Pseudo Genes (multiple problems)  :: 5 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..50570
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640370-rid9891663.denovo.007"
                     /isolate="E20834_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            277..1602
                     /locus_tag="IV334_000445"
     CDS             277..1602
                     /locus_tag="IV334_000445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HAP8942543.1"
                     /translation="MMDYSKEPVNDYFLIDMKSFYASVECIERNLDPLTTELVVMSRS
                     DNTGSGLILASSPEAKKRYGITNVSRPRDLPQPFPKTLHVVPPRMNLYIKRNMQVNNI
                     FRRYVADEDLLIYSIDESILKVTKSLNLFTTEETRSQRRKKLAQMIQERIKEELGLIA
                     TVGVGDNPLLAKLALDNEAKHNEGFIAEWTYENVPEKVWNIPEMTDFWGIGSRMKKRL
                     NQMGILSIRDLANWNPYTIKNRLGVIGLQLYFHANGIDRTDIAIPPEPTKEKSYGNSQ
                     VLPRDYTRRNEIELVVKEMAEQVAIRIRQHNCKTGCVHLNIGTSILETRPGFSHQMKI
                     PITDNTKELQNYCLFLFDKYYEGQEVRHVGITYSKLVYTDSLQLDLFSDPQKQINEEN
                     LDKIIDKIRQKYGFTSIVHASSMLESARSITRSTLVGGHAGGNGGIKND"
     gene            1595..1945
                     /locus_tag="IV334_000446"
     CDS             1595..1945
                     /locus_tag="IV334_000446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8942544.1"
                     /translation="MIDYNDRGRLKWAGFYLSDHTEEIDADNEQRTSQNLAKEQMTTE
                     EIAEVLNAAVIKNRSVSIQKEERNAEGYYPEDIVGKILGSDELGIYVGKDKIHYDEIR
                     HIEFYQDNKWSRLE"
     gene            1942..2121
                     /locus_tag="IV334_000447"
     CDS             1942..2121
                     /locus_tag="IV334_000447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001021554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8942545.1"
                     /translation="MNIIQQYELKYITFDQLSEKIWGYGQRLINEVGVERFSFYVEAA
                     AGYHNFRFYIFPLYI"
     gene            2257..2547
                     /locus_tag="IV334_000448"
     CDS             2257..2547
                     /locus_tag="IV334_000448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000026576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein PrgN"
                     /protein_id="HAP8942546.1"
                     /translation="MSINTFVYSHPINVYIIKNLGITVEQFCELYAYPQGTVASWITR
                     QRRIKSLPASFVYDLSLASSLNMSDVYEKLLSLEKEYDSFKIKHDKKIKKHI"
     gene            2915..3703
                     /locus_tag="IV334_000449"
     CDS             2915..3703
                     /locus_tag="IV334_000449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HAP8942547.1"
                     /translation="MARILSVANQKGGVSKTTTSVNLAACLKFVYGKNVLLVDIDPQG
                     NATDNVGFDIDGTNQPTIYEVLKGEADITDAILDYKGIDVLPADIALSSAEREFTQVG
                     SEHRLKRVLQPIKDNYDYIIIDCPPSLGMLTVNAFTVSDEIIIPVEAAYFSLKGLIKL
                     SETIETVKEYTNDKLEVRGVLFTKYNKRYNISKEMTTAATEISRVIGAKIFKTYIRRT
                     ITVDEAQAAGSDLINFNKPSTAEDDYKAFTEEYLKEVGDFDGRR"
     gene            3690..4016
                     /locus_tag="IV334_000450"
     CDS             3690..4016
                     /locus_tag="IV334_000450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8942548.1"
                     /translation="MAEDNRSQAAVSRLLGNKNKKNYKPKEVEEIELTKKNISKVIKD
                     QGSSVRTADSIRINPILMSALKYWTTIAESDKSKPDVIEDALLKSIPEEYLIEGYKLA
                     KKQNKI"
     gene            4365..5405
                     /locus_tag="IV334_000451"
     CDS             4365..5405
                     /locus_tag="IV334_000451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="HAP8942549.1"
                     /translation="MNDFNYYKSKEIYREKYYQMPKVFFTNEKYMDLSNDAKIAYMLL
                     KDRFDYSVKNNWVDSDDNIFFIFTVEELMKLLQCREGKVSKIKKELEKAGLLKQKRGR
                     VNKRDGKIESMPNLLYLGKPEVTNQDVFKIMEEEDNTDSTVIAKIANTAKPRKINDST
                     VIAKIANTAKSSHIKDSTVIAKIADNLFYSNSIDTNRHIIDTETDQLQNQVLLDNFVD
                     IMQEDSINTFVPENVLNLIKTFSSSYSEAQQTVQTIHNAKKKAEELEQTIVVYEELES
                     YGIDADKGLYMTLLKAYQKQKTEKVNNLQNLIFVYVKNWFVEKAIAAKLANEQRETFN
                     ELPTVSTENWLE"
     gene            6241..6573
                     /locus_tag="IV334_000452"
     CDS             6241..6573
                     /locus_tag="IV334_000452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraE"
                     /protein_id="HAP8942550.1"
                     /translation="MSYTGILSLEDICHYGKRCTATEKITKKLSTGQSKTVVQCKKYI
                     IQKDKVSEEMIYYIGKQKQIILKDPIPLKELYPTIKHVYDQNGVLIGRRKNGVLRCTA
                     KGMGRLIS"
     gene            7164..7493
                     /locus_tag="IV334_000453"
     CDS             7164..7493
                     /locus_tag="IV334_000453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8942551.1"
                     /translation="MIQKFAKMSKTAWIEKLENDLSVMRLAPKKFKREIAEKEAILSV
                     LNAVDDKCPVKISFSDIATIIEGIYYGEVDEEISSTKDLLDFLDKNKDTFIVLTDYEE
                     VTTNEKL"
     gene            7480..7821
                     /locus_tag="IV334_000454"
     CDS             7480..7821
                     /locus_tag="IV334_000454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5406 domain-containing protein"
                     /protein_id="HAP8942552.1"
                     /translation="MKNYDPNSRWGTHTIKVSFQRWDYKGFVTFRRGGNCKGLDVLAL
                     DEDDLYDQKLTDNPIGFGLLPEDDQGNEWFKMTLMNDNGDELSVEDTWSYLSDYIVSV
                     EIIEFVADKEE"
     gene            7935..8174
                     /locus_tag="IV334_000455"
     CDS             7935..8174
                     /locus_tag="IV334_000455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8942553.1"
                     /translation="MARPVLEAMDMDTLTKGRYVQLLARGYSPKEVFKSLSKGTDMEK
                     ERLRKEFDYWRTHNGIKDLKPARPVIKRKKKITQD"
     gene            8241..8480
                     /locus_tag="IV334_000456"
     CDS             8241..8480
                     /locus_tag="IV334_000456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8942554.1"
                     /translation="MNYEEIENRKKVSKEMEEKLLKTMKQKHLKRLSVMQYINDMQIT
                     GKEKACLLGSMKNFEQLRRTYVKKSSNCQLLLEVS"
     gene            8566..9237
                     /locus_tag="IV334_000457"
     CDS             8566..9237
                     /locus_tag="IV334_000457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A sortase"
                     /protein_id="HAP8942555.1"
                     /translation="MRKWLFGFLGVALIVFCSVFGYVSYQKHEGKVFKQNIEKKMPVD
                     QINEHAKSYKEDATNVNNDMSLGQMLSIQKEAIEMGVNKQAFAQIQIPALGLALPIFK
                     GANQYTLSLGAATYFYEDAEMGKGNYVLAGHNMEMPGVLFSDIQKLSLGEVMDLVSND
                     GVYRYKVTRKFIVPEYFKLIDGVPEENSFLSLPKKGEKPLLTLFTCVYTSQGKERYVV
                     QGELQ"
     gene            9290..11266
                     /locus_tag="IV334_000458"
     CDS             9290..11266
                     /locus_tag="IV334_000458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopeptide-forming domain-containing fimbrial
                     protein"
                     /protein_id="HAP8942556.1"
                     /translation="MAINRYVKKAGMLVGMLGIIASCFQGYQVHAEDVTQKTPPEKVN
                     ITVHKLMYDQGTQLNVDIDGIKNDGYTHDQYPTGVTKYNKADYGDVEFTLGNITDQVL
                     PREDSDLTNAKVDEIVQDVEDKGSNSEYVKNATNKITSAVDENGEITFTDQPAYVNSK
                     GNVYVVYESKSAAGLVTQKAKPMVVIAPMTDKTGSGFLKNIHLYPKNIVSKLSFELTK
                     FGDDGAAQSKQTLLKGAKFELYKGEPGKGTKLGDLVSDDQGKLTATDLTLGKYYFVEV
                     PSEVVVGSDQEPTADQYLLGADARNDAHNKLTFEITNDGVTSDLKASYVNYKAPVIDK
                     TVTNGTGQEHSFQIGDAVNYQGTIHIPTDIAGGTDGITVNGVKSETSPYSVFKWGDTA
                     GQGLSYVAAKANIKVTNKDGSVVLKENTDYKIQNSENGFVIDFIVNNGQVSDTVASLH
                     GQDLQMTYNMYVNDSAAVANPLTNSVDFVYNNNPFNQEEHHEKTKADVVTYGAKFLKV
                     DSGLFGTGIKATPLEGAEFAAKNAEGKYYGGLVDTDKDGVKEAVWVDDVANAAILKSD
                     KEGHFEITGLTEGEYSLEETKAPTGYQKLTEDIAFKVNKNSFKEENRITVKNNQKAAI
                     PLTGSNGFQTYVLISCLLLGAGALSAVVYFKKKA"
     gene            11279..11935
                     /locus_tag="IV334_000459"
     CDS             11279..11935
                     /locus_tag="IV334_000459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HAP8942557.1"
                     /translation="MIIRKPPKRRVMHKLMIFFWLLSAVAVYLIPAVETNVAKFQQQQ
                     RILAIENLEKTDSSLDVAKIELGDPVGVLTIPSIPLKLPIYDGTSDKILENGVGITEG
                     TGDITGGNGKNPLIAGHSGLYKDNLFDDLPSVKKGQKFFIKVNEEQHAYQIYRIEEVK
                     AEELQKNYLDYLMPEKDQDRITLMTCTPKGVNSHRFLVYGERVSFSKEDLKLQKKAKK
                     "
     gene            12031..12858
                     /locus_tag="IV334_000460"
     CDS             12031..12858
                     /locus_tag="IV334_000460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8942558.1"
                     /translation="MNKRKWLFVCVAALFATAAYSQISFADSSTTPTTSQTTDSSTTA
                     TSDTDSSKNTAEETADSSTSSTESAESTSESSSETSEKLQEVEDTQVIKQNIHIQKII
                     SSNRINNQGRQVTQQEGVNGAKFIVYDITNILEEMTQDDNEKNQSVEIIESKLKDRAK
                     KLSYDQLKKVTEGETQTIDDQEGVIDFSIEVPANKKQAYYIVNESSPENISNSEDIVL
                     LTPVSDEKGEFLTDVWLYPKSKKSEPKEEIKKVVSTGVKKNFFDNCWDFFTHLFFND"
     gene            12868..13116
                     /locus_tag="IV334_000461"
     CDS             12868..13116
                     /locus_tag="IV334_000461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8942559.1"
                     /translation="MKIIKMLVFNLSFLAATILCCGLVFSQLSIYPRYQRLFVHFLNI
                     QHASMPLEVINKLLINPIFMASLFLLAGLVTWVLGKKE"
     gene            13147..15258
                     /locus_tag="IV334_000462"
     CDS             13147..15258
                     /locus_tag="IV334_000462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopeptide-forming domain-containing fimbrial
                     protein"
                     /protein_id="HAP8942560.1"
                     /translation="MEGIMENKIHCTTGRVFTLVLFLFLLLFARGTAVQALEKVDPSQ
                     IKENNNLVLYHDSDSLRNIEVTGINGFKILDEYSTKKQKVLAWPKVKDLKSTSKIVAH
                     YQKVGTYLGKEIDCDVTFSNFVAHAGVNQDGLAKTINGKKYYNYLLFDKNIYRGFFMQ
                     SINVMNVKLDFKYAGSKTKVALSEPQPEINSKMRSMRAAGIELNDTFLSIYSLNGQYQ
                     QRVSWYNDNNESVGERNIDMREFVGYEKMDRLPYFVTTDTACAEYKAPALSTNNLTSK
                     VVGGRAEKAYSSNDHFTDVVGADTYHRSGVTYTLQGASPTFMIGANTNSMMFSFDTAL
                     LWTPQPSKPTKEVFNKANTEEAAHNIDKKVIPQGSDVYYHIHQKFDALTVNTMNKYKS
                     FKITDTFDSKNFDMVSDGKNYDGALWYVEANGTWKKADTGGKLTVSGNTVTYTASDSF
                     LANAANYGRDYILQLHMRAKNDAKGIAPNVATVTFNNDPNKGTQKTNKVENYFSLKPN
                     KKVEQDGKDVDGRNDGKKGTPTAPLNAGSEVQYLVTQKWHTKGVDIALDHYKQFSIQD
                     PVEARLTYKEGSARVIDMSTGKDITSEGILTYDSHSRTLKWEASADFLNNNLLDGREI
                     QLIFTAKTPLNEELDIDNQAKVSVESIEKATNVVTIGVKPNLPQVIVPKTGSNHLVLT
                     VIGSILFLTLASLGYVVLKLK"
     gene            complement(15296..17353)
                     /locus_tag="IV334_000463"
     CDS             complement(15296..17353)
                     /locus_tag="IV334_000463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HAP8942561.1"
                     /translation="MKLFNPKTVLAIGTFAASLSLAGSVDAASTNVMYRLYNPNNNEH
                     FYTSSKKERDHLVKIKWGNYEGPAWEAPTSGGHLVYRLYNKGLRDHHYTASWDEVKWL
                     TKNYGWTYEGPAWRSAEKNNKPIYRLFLPGVKSGSHHYTASWKEVQWLTKDYGWKYEG
                     IGWYGADTSKPKVNKSALEALYTSVKGTKKGDYTDNTWNAFQTALNNAKKVLNDSKAT
                     QKQVDSAKNKLDSAYKGLKKKPVPTVNKAELQALYNRVKSTAKGDFTEESWNNFQTAL
                     SNAKKVLDDSKVSQTQVNNAKNSLDAAFKGLQHKPETPKEYMVTINHIDNQTGQVIAT
                     DQAKVTSGSTYTAKAKAFKYSEGAKDNFSYKVIGNDTQSKTITGNTTITFNYNQVHQI
                     NLLCNNNYKNEGRVDILKEQIINVTHGESVTLTAPSIQGYVLDDRVEPTNSVTLNNVT
                     ESQNYIFNYTRKFNVTVNHVNADTNAVLSSESKTVYEGDSFSTPWKNMTDQNYFLCRN
                     DDPTVTVDKNGQRSINNVDQNKTITFKYKKISLDELRNFMRQKELAWLNDYRQQNGVA
                     PMQFNDIVQQAADIRAKELQVSFSHYRPGGGTFQDLLESLGCYGAKGENISQTGLYID
                     DLLSDDGAIDAMVGWKNSPAHNANLLYNNQSILGLGHNFVVDSAGRIGVDNVFISANP
                     IFK"
     gene            complement(17467..17679)
                     /locus_tag="IV334_000464"
     CDS             complement(17467..17679)
                     /locus_tag="IV334_000464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8942562.1"
                     /translation="MYADKKKIEWLLFDSGKSVRSISIESGVPLTTVSDLANKRSSID
                     NMRFINAMKLNKLAEEIVDINTSTNK"
     gene            17866..18333
                     /locus_tag="IV334_000465"
     CDS             17866..18333
                     /locus_tag="IV334_000465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8942563.1"
                     /translation="MKKKQLWFVSMFAIFFIILSGCSSNKKSEAKEDNSLLQPGTVWK
                     EEVGGLIHNLKIIDDTTWEYSESVWHPDPVQITVKRQKDYEGLERYKIVDSAGIREFI
                     NKPDSLFIVVPYEKDGVKKIIFLGSSKDEKRTKEKLIHDGSQSNKYKLQKTSE"
     gene            18330..18572
                     /locus_tag="IV334_000466"
     CDS             18330..18572
                     /locus_tag="IV334_000466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8942564.1"
                     /translation="MIASLFICPEGGITVAKAKTPFEKAASKILERRGVSETQWKQEV
                     LEKAQFDLLRGKDKDLEGYIYQREREKLVFDEIIKH"
     gene            18590..18892
                     /locus_tag="IV334_000467"
     CDS             18590..18892
                     /locus_tag="IV334_000467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8942565.1"
                     /translation="MFESITMNFLTQPKVVAAVDIFRALQTAATSLKNNLMIFAAVAA
                     VASIALGGIFFFFGRRGAENGKTHVGYVLLGVGVILAAASIVVSMFSMFGQTPQGL"
     gene            18963..21017
                     /locus_tag="IV334_000468"
     CDS             18963..21017
                     /locus_tag="IV334_000468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8942566.1"
                     /translation="MFFTISGNAKLDKELIRKATKKVGKRTSFLPKTIKYSVIETEEQ
                     LHVLGQVLEQENQSSVKLTLTLHDKEGKIEKAGTEYVFGSLDYQISEGFYPTLIDLIR
                     LDIFKDTDETMSVEESYRNEKILQVLEEELESDQRKVNQVSEVEANEEVPWERPIFPK
                     DESKEKEKGIIDEQADILTHKSIFEEENEVMGQLEVDPFEETMEDEETAEVEVDPVEA
                     NEDPTWTEGDSQEYIEPLISEEVEKNETEIQKATVFDYILDQYPPEHEWMKEKNKLFL
                     NELYSKYQLPQTYQAFLESKEELISQGKGTLLEQLESVQSTNWHELAQEDLLATFEER
                     QQETDQEINEYATEQSSNWEKAKEELNKEEQRVIEEEVQKIKERYEKKRDNAYSLCVE
                     KINNFTQTNQEALSKEKENLLKERIKERKEEFYRGLRTDKLRISKELNEQLNKLYQTT
                     TEVLMEKHEQIQQELQAQIPKWKKEYEIHQQELKEQEEKQRKKEKEREQLELKKREQD
                     LAEQKMAIEKQRLEQEKEKEAAARKERQEQMDTLRQAQLNYLTHPQPVAAVQTPVNNQ
                     ANLTPSPQRSFNGWMVGCIASLSLLLGGGTVFAFNHINQSSQADSVATTASIKEEAQE
                     QARKEYEQQLEELLNRTNQSTTESSMENSKESDSSKAKEGSSDSTVQSNTSESSESNE
                     VK"
     gene            21017..22849
                     /locus_tag="IV334_000469"
     CDS             21017..22849
                     /locus_tag="IV334_000469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretory system conjugative DNA
                     transfer family protein"
                     /protein_id="HAP8942567.1"
                     /translation="MRVRETTKRKDKRFNLEKNRYLSHFYRYTPSGEELPEKHDKRIS
                     KKIFYSGLVYLPLEYLVTRVPEVFNRLEIVKGSLQGINLFQLQEMPQVFHFDSAGFWN
                     PFTPTTSLGTRLAISTVCVVPAVLMGLKMDMWFRPLADGQKGDNRLMTLKEIQQIYPQ
                     IPETKTTFPGYGGIPITHYKKYWYIQTDTVNTCIVGTSRSGKGQIEVLATIDNLSRAE
                     KQSSMVVNDPKTELYVGSKDELEARGYDVYAFNITDPLQSMSDNPLALIVKYWKRGDI
                     DTATQLTNTFTNTIYYDANASDNKYFNENAQKAVNALIFALLEQADQTGDYSKLTPNN
                     LVELLSEIGGFNYYPDPDNEFKQLNALDEFMNQLPPGNIAKKQYGATKIGSDKAKGNI
                     LSTAITGLNPFTLTKIAKMTSQSTIDYKSVGFPKYLDLKLHESLLNQRVVVEFYHKGK
                     KFHEERVKVGYKGFSEINFDCHLVAGDQVCLKYDHQGKEYVAWYEFSQRVLKDEEGNI
                     VYRKKRGETHLPELEKEAVLTLDEEKSNLLVESIRMHYTDKPMAIFMLTPDYDPSNHI
                     IVSIFLSQLYKELSTQCVKTKGDKCHRRIHFILDEFGNSVTCLA"
     gene            23544..25460
                     /gene="ltrA"
                     /locus_tag="IV334_000470"
     CDS             23544..25460
                     /gene="ltrA"
                     /locus_tag="IV334_000470"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HAP8942568.1"
                     /translation="MERDKTMTNTKGISLKNKQLRHAEYYDMTETFDELHAMSVAKSN
                     FKNLMKFIIDDNNIFLAYRNIKKNKGSATAGYDGVTIKDIEKLACGDFLTIIKRRFKD
                     YRPRKVKRVEIPKPNGKLRPLGIPSIWDRIVQQCILQILEPICEAKFNKHSYGFRPNC
                     STENAIADCLARINQSKMNYVVDIDIKGFFDEVNHTKLMRQIWTMGIRDKQLLVIIRK
                     MLKAPIVMRNGNVVFPTKGTPQGGILSPLLANICLNEFDWWIANQWEERTVKELKHHY
                     RKNGAWDKGNIYSKLRSHTSLKEMYLVRYADDFKIFCRTGEEAQRAFYATQKWLRERL
                     KLPISMEKSKVTNLNKAESEFLGFSIRLHKKGKKKVAFTYVCRKAEENIKNKLKQQIK
                     KIQYAGAKKNRHRAIGDYNMMVIGIHNYYRIASHCSVNFTEIYRSVRTVIEARLKARG
                     MTKNGIYGQDHEGYRPYLKSKQMRYIEGFPILPIGYIKKRTPLNKRRNLNKYTKEGRK
                     LIHQEQQAIESWKIKYLVKGFSNNKKYTVEFSDNKISKFISQKGKCAITGNELLMEEI
                     ECHHINPYHLSKDDSYKNLVIISREIHRLIHATSEETILKLLRLLKLNHYQIRKLNTY
                     RKKAANFPISIKMN"
     gene            25577..26377
                     /locus_tag="IV334_000471"
                     /pseudo
     CDS             25577..26377
                     /locus_tag="IV334_000471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628759.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TraM recognition domain-containing protein"
     gene            26374..28905
                     /locus_tag="IV334_000472"
     CDS             26374..28905
                     /locus_tag="IV334_000472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8942569.1"
                     /translation="MIIGGIFSGIADWWSGLFGEGDANPKTVTKVLQDYGNYLQYQDV
                     FSWICNTFLWGLIKGLYNLNTLLEKTIYQSFDLKDLLNAAGVNELFQSLISKVLALFC
                     VVTLIYLGLKFFMSKHPPKVKNILVNLFMAMILIFGGSSLIDEGLNISKSFYGDVTAA
                     NKADKTGSPAFQIIQNNVIDVSTLLATDPNKVESLPTDKRNALTAKNFKTANINGIIT
                     PKQAKEMADKDKSGLSDLQKDRLRALQYRLELDDNGKEIPVQISDEGLAKYVYTSGYR
                     RYISSPGIILAGESSLAVAYLFILFTIVTCIIELVFKKFYLVIAASTDFETGQRMKSA
                     IEDISQSFLLLAFTILELRIYTLMLSGIGDLHAAGKINGFLYVVALIVLTIALFKGSQ
                     SVTKIFGVDTSLKNGSNSLLSMFALGNIAKNVGSGAKNLASAGKSGLSHLNDIRKNGF
                     GHRKDVTPDNGEEQSDDKATTVPNSKKTVSEMFGKAKNGVSKAAEGLGYVNERGLKGT
                     AEDLTEKAKDTLEDKLDGTRAKELAEAVKNPSEALEKQKEKASEVKEGLKDAYGEGQV
                     NAAVKANTTTEKENQEGIVIPSVKDHQEALHSNENSIEEKNSIEERNSEEGKNGQLPK
                     TDPEQYRYKPNSDLENEKRISDLSKVNQEDKAENEGQLPKTDPEQYRYRSVNSENEKR
                     ISDLPKRNQEVQTDEDSIVLPNDNGIEVGTEKSTKIGLPKAQNLPMKDSRLNQFKDND
                     QSFVHQSPAGTVQMKPSRLPENGNLTTVSYNLSESGETGNASVQNVVTREAAPKPIQN
                     VHTGYKPANVAFTAPTKINVTRETKNRNNALKEQLLRDIKQDIKP"
     gene            28955..29287
                     /locus_tag="IV334_000473"
     CDS             28955..29287
                     /locus_tag="IV334_000473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8942570.1"
                     /translation="MDKYINMKNPPRFSGEWKFFAFFFLDLLTLGGCFFWAKMVNNLV
                     HLPLFFALLNYGLWIAIALVIVWRPYNNPGQRQIFAIIESIFFADKNHYHALDPNRNY
                     HGTYKEDE"
     gene            29288..29881
                     /locus_tag="IV334_000474"
     CDS             29288..29881
                     /locus_tag="IV334_000474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8942571.1"
                     /translation="MRVKGQNSVERKSILQFGKPEERSILDLFPYKSCTEEGVLITRE
                     DHFQRYYRVVSTDVEGLNEQEKLERMNQLTTVMRTYVPTIKLVSLTTETDLTEQIVQK
                     RQLLDKNRMEQSLGRNLQRLRKYEKKLVEEIQELKRAEQERPDLSFFFVIEGKTIKDL
                     QTRNRQLMRSSGILGLKPLYKKELIKILYRMNNMNDE"
     gene            30044..31738
                     /locus_tag="IV334_000475"
     CDS             30044..31738
                     /locus_tag="IV334_000475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HAP8942572.1"
                     /translation="MLEAIKTAFSPQNDQKVLDPLKQLGNREIEILKSKGYDLDFLKQ
                     VMPQGGLNFDEEYVTYGNGYSRCVTVYHYTKNPTLFWLANLMNNPHTVATLDVSTDEK
                     QKVIDQINRALDELNDRSDNERKATDRNKSAEEYLELAEYARKLTSNGEISKQVKVRV
                     HVYGNTIQEVDERAQELIENLKGMDYRAVTYLHKTKRDWQSMFTMFDEQAEWFGNIKG
                     QSLPSLNIGGGFPFHHQTLKDPGGTQYGETMTGGPFVWNATYKNSVRLSTNTLLLGMM
                     GAGKSTVLKMIAEAHLAAGDFIWGFEKGKDFIPFLKEYNGIMVRLDGSDGMINPLEIF
                     ATRTYDEASSLYDDGSVKDLKINEAASYQTHLDKVVYQVQLVSPQLKGTMKAEFKTYL
                     NRFYEEYGTVPRGFTSSNSRSNTDTQVTGKDPEAYPTFKEFLDFLGQLELPGASQEKK
                     NRKEEMESIVESLCETYGMIFDGHSTIRHLDQQQLVFFDIDSITGLDRGVQQCQMYMA
                     MTLIWNQALIQGRRQRRLIKEKQMEEQDFKCFLAMIDECHNGATCSAVKSCGLQVV"
     gene            32426..34303
                     /gene="ltrA"
                     /locus_tag="IV334_000476"
     CDS             32426..34303
                     /gene="ltrA"
                     /locus_tag="IV334_000476"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HAP8942573.1"
                     /translation="MTKETSPKDRKLRHAEYYGMTEIFDKIYSKSLKGDNFKNLMQII
                     ISDNNIVLAYRNIKRNHGSVTRGEDGVTIKDIENLNRNEFVEIVRKRFAHYNPRKVRR
                     VEIPKYNGKLRPLGIPSIWDRIAQQCILQVLEPICEAKFNNHSYGFRPNRSAEHAIAD
                     CMARMNQSHMEYVVDVDIKGFFDEVNHTKLMRQIWSMGIRDKQLLLIIRKMLRAPVVL
                     PNGKLIYPTKGTPQGGILSPLLANINLNEFDWWIANQWEEKDAKELKHHFRKNGAWDK
                     GNIYHKLRKSTTLKEIYLVRYADDFKIFCRNYNEAKKVFYATKMWLQERLKLPISEEK
                     SKITNLSKEESEFLGFTMKLVTKGNKKVVNTHIGTKAQSMIKVKLKKQIKRIQKSPNS
                     KDTFIYIGVYNSMVIGIHNYFKIATHCSVAMRKIARELRPSFEIRLREQGISKEGQYG
                     KYEGYKPYLRSGQIRYIRGFPILPVGYIQHRHPMCKKRNINKYTEEGREAIHKKQQTV
                     EEWKISWLCSNPVLSKRATIEFADNRISKFIAQKGRCAVTGEELILSEMHCHHIIPYH
                     ESKSDSYENLVIVTEEVHRVIHATQSETIEELLKYLKLNPKQKEKLNELRLKVGNEEI
                     S"
     gene            34434..34811
                     /locus_tag="IV334_000477"
                     /pseudo
     CDS             34434..34811
                     /locus_tag="IV334_000477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307536.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
     gene            34811..36016
                     /locus_tag="IV334_000478"
     CDS             34811..36016
                     /locus_tag="IV334_000478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HAP8942574.1"
                     /translation="MENQNESLIKQYVKRRAKRRLFLWLFGTSAGLITILITVFVTLF
                     LILAAGSIDNSDSDSSSGGEAFTGEYSEGLPIYKEIKGRGPFSDEIAQYAVGAAVKYK
                     LLPSVILSQYGYESAFGTSASARNDLNYFGITWFDGCLFPKGTARGIGGIEGGWYMKF
                     PNSKAAFSYYGFMVATQSNFNACVGNKSPGASLLILGRGGYAAAGITEDSPYYTGCMS
                     IITSNKLTEYDEFAIKHWGEGGNNNGTITGEWTNPFPGSSLDKSSFSGGQLFGTNPGG
                     EFRPNGFHDGLDFGSVDHPGSEIHAVHGGKVVYVGNPGISGLGACVIVINYDGLNMVY
                     QEFANSTGNSRVKVGDQVKVGQVIGIRDTAHLHLGFTRMDWRQAQGHAFTDDGTWIDP
                     LPSLNSSKK"
     gene            36032..36676
                     /locus_tag="IV334_000479"
     CDS             36032..36676
                     /locus_tag="IV334_000479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8942575.1"
                     /translation="MENKTLKIVLTAAISVSLIGNIALFWQLKEEQETNTFKIEEVAK
                     NASEKLKKNSSEAQAKIDKLNQTINEKNQEIDQLKQQDGTKAKEEVTEAQRKTAEEFG
                     QLAIDKSLSRNELTEKLKDVATQEVIDKLSPADDDHQHDDYGSYSFTLGDVQTYAQTS
                     SVKEEQNFVVFVDYTIGNPQFKDVKPQKIKGGLTVTEKQVDGQWKVIDFSYFTR"
     gene            36687..37493
                     /locus_tag="IV334_000480"
     CDS             36687..37493
                     /locus_tag="IV334_000480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8942576.1"
                     /translation="MSKKKGERQRFKKRKSYLQWLGIFMILGFLILASPIYTNQKVDI
                     PDEVSTNAFLNTKELTMVSKEKGANQQLVFEFAIDDEDQSLLKELANLNYRVEVKTAK
                     GDYQAIRAKVIKVSDDYFAVKLTHVPEEYIAMRLTIIPEKIDPKVDMQEPQDLIYYVH
                     EDKVKDQVKDEDYEQHAINYKVKGYKKEQKAAQKQMESFQATIDLNEELVKKLKDQLP
                     YQVADDQENTENKINSYQQEIETSKTQLKDQEEIIQKLQEKIDRMTKEKI"
     gene            37515..37745
                     /locus_tag="IV334_000481"
     CDS             37515..37745
                     /locus_tag="IV334_000481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010731508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8942577.1"
                     /translation="MKRTNLADKDTFAEQKVLIKAYLKVVKAKEICREVNLSNTTISE
                     LKNGRKNLDTLKFRSFEALYVAAKKYFTADNK"
     gene            38291..38566
                     /locus_tag="IV334_000482"
     CDS             38291..38566
                     /locus_tag="IV334_000482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8942578.1"
                     /translation="MDLLIRNIEEKYINKIDKRCEELTVKTGKKWSRNQYLKVLIEND
                     FDHALLNYKKDQFDRLLEKFVDIQTYNTKTLEEYIATNNQLIGLLIE"
     gene            38608..39030
                     /locus_tag="IV334_000483"
     CDS             38608..39030
                     /locus_tag="IV334_000483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8942579.1"
                     /translation="MAKATRKHVYLYDMHIEVLDEIISTHEDISNYSEAVRHLCMMYD
                     PKIEEKINPIQIKLNAMGKEISILTEMVSEFADLHLKDTGILIGTNSTVYQEAEERVE
                     GKIKSNQTKKYSSKLKKPVNESVHTRVDPFDPTKILKR"
     gene            39049..40641
                     /locus_tag="IV334_000484"
     CDS             39049..40641
                     /locus_tag="IV334_000484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8942580.1"
                     /translation="MNKSPAVILTCQFTLPNKKSFSTYIDYMTREKALEEMERRTPEE
                     EQELQRIKNALEDFYIPEGETFSESKTDKEFSETSNEAQSLMEHGINFDKLEEQDFTK
                     YLSYMTRHYALEQKKELTQREQKEYQRLNQAMKKYQTEPHKKASGNLPGVFSMASDEV
                     KGSDLKEIKQIFQRGQQKGSIIYQDVISHDNSYLEKMGLYDPKTDTLDEEGLKAAGRK
                     MMETLFEKEQLNETGYWMATIHRNTKHIHIHFAVVEKENTRKPYTEIENGVSYIVPKG
                     KRRQATLDAMKTNYVHELERFSYEKDRMIGMEKRNLLTRKSDLRNTLTKVVKEPARYD
                     RHAMNLLNEVYKTLPEKRSEWNYGDENRTKLSPFTRGKLDELTKYVLTKEPGYQEYIQ
                     ISKALKEEAKSLYGESKRETKNAEKNALFDLKKRTGNAILTELKRQDSQIKPLIESVN
                     QMQSIYNERSFRTLKQLPTNEYKYSYQEFVKRRKQFQQRVVTQRSLRQLNRTINAHEE
                     KYHASKAYEEQQRRIQMEQERQ"
     gene            40653..40988
                     /locus_tag="IV334_000485"
     CDS             40653..40988
                     /locus_tag="IV334_000485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8942581.1"
                     /translation="MKQYLDQWKVIEGSLKEERIEQLPDCLEKEHLFQISEMLRNEQF
                     DPNQFLVVEYPATGVYCCNHVKGEKYFIIQEYEGKLAPYYTTWEINEEGINNFPCESI
                     EESISLTEC"
     gene            41135..41329
                     /locus_tag="IV334_000486"
     CDS             41135..41329
                     /locus_tag="IV334_000486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8942582.1"
                     /translation="MTQTTIGVVVEKSRDNLVFVTEIQTGRAFVVTDKAAKAYQNGNI
                     LTLNLTTKMFVDAVENYPFV"
     gene            41361..43733
                     /locus_tag="IV334_000487"
     CDS             41361..43733
                     /locus_tag="IV334_000487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8942583.1"
                     /translation="MAEEKEPLDNSRLGKSKRKLVRLQNELNNQFQKLFDHQKLTNGQ
                     PMNDKRNGRSWFNQQEKIENKIQSLREEIKQQEKQVEKLERQEELKEMGYNKYGGLDM
                     TIENIPRIKEEIERFEKGESTFSAATIRKYQRKLETLEQLKEKSEKGKENLLPEVQAI
                     IDSGRVTQWKKNPTIYFLKGYRKVALELNENGGFEESKKYQATSEEEKAAVRELLEEA
                     TPTDSVKEKETDHFYRVEFNENDPAKGLKSYGGEIVTLELIDQLKTLDNQYAAEAGYY
                     KFYFEEVKNGEVVDQIRMDLGDGVNSNHPIYLELARLCEQSPEKEQEQTVKNEPISKV
                     QNEIKETKTKQKGYQKRTAEEIKQEVKSLSQQALDAVKKHTHSPEDVKELLNFMSRFP
                     ERSFRNQLLIEEQFPGATACLGRAALKKEGIYIKKGEQGNKIFVRKTVKGFYEKGRGF
                     VRETEATPEDKKRIESGQIEVIKKPYYTIEKVFDITQTQLKPEDYPKIFPNRVFDFQL
                     DQTGQTELQVGIQAVAKNIGIQIRDMTESEVYQRELGQARGAYVRNEFTGEEEIVMNT
                     RNTPTQHLATSIHELAHARMHKFSELDTATKELQAEMTSYIVCKHFGMDTSEKAIPYI
                     ATWTKNSQTLDDKEAAERGKIMNDVSRVANEFIQTISNEINQYREREPEIQPAEVKQE
                     HKNTVETAEQKNIVPVGSLWIDKKDGKVLEEDTGRTLHLKDAAFIEDPQHNQVILKGK
                     SYGKEIQTALPSEEFALGNPFRFSLETVSEVAKRKNEIYYEQQLSQKERV"
     gene            43794..45254
                     /locus_tag="IV334_000488"
     CDS             43794..45254
                     /locus_tag="IV334_000488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HAP8942584.1"
                     /translation="MTEKKMSLIDRCKQMDIVDFARNNGLAVANKGRDYRLEDHDSFV
                     FDRRKQRFYWNSQNISGDIIELAKLFFIDKEIQDPKQQFKAATDFILKNEDKTERVEN
                     LHFETEKYKDHPVDYQPLTEKGRNYLKEERKLPDWLIDYAEKEGLIAELKPKHERQNF
                     LVRDDRLDHAVAFLWKDPQTKETVGASYQGTFIDYERFGERGTYKHIDKNSTANHGFN
                     LKIGDPKQLKFFESSIDLLSYAALNRDQLNDTWLVSMEGLKHHVISHYFGEAVSELRK
                     KQAFPQSIEICVDNDRAGHIFYEKEQLMGAVDPFTNQKVRCERGIANDWQVPREYKVI
                     YEEVAKEEKVTPEAIMAIHKTENNLQLTNQLVSAHKVNASFGQQLSVNDSIEVISLKD
                     ICREVAKELKDCERSNGTYDFDRFYQGKGDINSQILFSYKAEQYYKGYKNHEHEFVPE
                     VKKDWNDQLKHEIHQQEIRKQKRAMLFQQGRQQERE"
     gene            45265..47469
                     /gene="topB"
                     /locus_tag="IV334_000489"
     CDS             45265..47469
                     /gene="topB"
                     /locus_tag="IV334_000489"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HAP8942585.1"
                     /translation="MKTVILAEKPSQAKAYADSFSKATRKDGYFEIQDRLFSGETVIT
                     YGFGHLVELDSPDMYDENWKQWSLEHLPIFPTHYHYHVPKDKKKQFNVVKQQLQSADT
                     IIIATDSDREGELIAWTIIQQAGADQGKTFKRLWINSLEKEAIYQGFQQLRDAEETYP
                     KFEEAQARQIADWLIGMNGSPLYSLLLQQKGIPGSFSLGRVQTPTLYMIYQLQEKIRN
                     FQKEPYFEGKAQVTAQNGIFDAKLDPNETQATQEAFEAYLKEKGVQVGKQPGTILEVE
                     TEKKSAASPRLFSLSSLQSKMNQLMKASAKDTLEAMQGLYEGKYLSYPRTDTPYITEG
                     EHAYLLDHLDEYKHFLKAESFPTPIHTPNSRYVNNKKVQEHYAIIPTKTVMTEATYEK
                     LTPLQQAIYDQVLKTTVAMFAEKYTYEETTILTQVQQLQLKAVGKVPVDLGWKKLFGK
                     ESDRKEKEEEPLLPKVTKGETVTVDLQIFEKETKPPQPYTEGTLITAMKTAGKTVDSE
                     EAQSILKEVEGIGTEATRANIIETLKQKEYIKVEKNKLVVTNKGILLCQAVEKEPLLT
                     SAEMTAKWESYLTKIGEQKGTQATFLANIQKFVSHLLEVVPGQIQATDFGATLQEVKV
                     ASEKQEAARHLGSCPKCQEKEVILYQKVATCTSEACDFKLWVTIAKKKLTATQLKEII
                     QNGRTSQPVRGLKGQKGSFEATIVLKEDFTTGFEFSEKKKTTYKRKVRRKTS"
     gene            47715..48308
                     /locus_tag="IV334_000490"
     CDS             47715..48308
                     /locus_tag="IV334_000490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HAP8942586.1"
                     /translation="MKDKVKCIFEKYNGNLSLKDARNEGLSPMTLDRLAEKDKIEKIA
                     SGFYTLKDSFVDELYLAQSIYTRGIFSHETALDLFQVGTYIPKKINMMFPKGYNVSKE
                     NLDQYAIQPHYTNKENFELGITEAESFYGNKIFVYDLERTLCDMWNPRYKASVEVKQE
                     ALKEYMTSTSRNTNKLRRYMNILKSPKEMTLYMLPLY"
     gene            48324..49223
                     /locus_tag="IV334_000491"
     CDS             48324..49223
                     /locus_tag="IV334_000491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HAP8942587.1"
                     /translation="MTPTQFKAKTRNVAAEKHLNPQIVQRYYMMEKLLECIVESPYHD
                     DFVLKGGFLIGSKYGLENRSTVDIDTTYRNSKLTKEKLETVFSELTAAPTKDGIRFEL
                     KGLTETREADYYPGFQAKFIAFLENARVPFKLDITSGDSIIPAALIYEHKLMFEDKTI
                     HIPAYPTEQILAEKLHATFSFGIDNSRMKDFYDLYMIPKLERIDEINLYKSVQGTFKE
                     RNNQSVFKNLYNQEMPQLMKNEGLREKWKRYQQENYFAKDISFESTLTSVDSLMQSIV
                     EQEEVERKQNFKRSQQMNQEMER"
     gene            49226..49711
                     /gene="ssb"
                     /locus_tag="IV334_000492"
     CDS             49226..49711
                     /gene="ssb"
                     /locus_tag="IV334_000492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016186236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HAP8942588.1"
                     /translation="MINNVTLVGRLTKDPDLRYTASGTAVTTFTLAVNRNFTNQSGNR
                     EADFINCVIWRKPAETIATLAKKGSLIGVVGRIQTRTYDNQQGQRVYVTEVVADNFQL
                     LESKAATESRMHADQSSSSPSPTTFEQRDTATPNNNGLNASQNPFGGQSIDISDDDLP
                     F"
     gene            50077..50337
                     /locus_tag="IV334_000493"
     CDS             50077..50337
                     /locus_tag="IV334_000493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8942589.1"
                     /translation="MNKYVGLLDKIRVIKTQPLLVRFTLQTIHESINCVVADIEIIDK
                     LLIMDDGKYNIAVTGHFNKRNQLVIASMYVRNPDHFTRSMGI"
BASE COUNT        18679 a         7921 c        10206 g        13764 t
ORIGIN      
        1 tcaaatgttg taaggatttt attcatttgt taatattaaa gaacggaact ccaaaaaagt
       61 ccgtactcta atttgcagtc tctcactctt ttagagtgag agtttttatt agattttcaa
      121 attaattagt aaagtgatag aataaattga tcatacaaat cataagaaga agcagggtga
      181 tagcatacct atgcttcttt ttttatgtat ttagctattt cttctttaca acatcgaaca
      241 tatgtttgta taatgtggaa gaatggagtg ttatttatga tggattattc aaaagagcca
      301 gtaaatgatt actttttaat cgatatgaaa agcttttacg caagtgtcga atgtatagaa
      361 agaaatttag atccattaac aacagaactt gttgttatga gtcgatctga taatactggt
      421 tcaggattga tattagcttc ttctcctgaa gcaaaaaagc ggtatggtat tacaaatgtg
      481 agtagaccac gtgacttacc acaaccattt cctaaaacac tacacgttgt tccaccacgt
      541 atgaacctat atatcaagcg aaatatgcag gtaaataata ttttcagaag atatgtggct
      601 gatgaagatc tactgattta ctcgatcgat gaatcgattt taaaagtgac aaaatcactg
      661 aatcttttta cgactgaaga aacacgaagt cagcgaagga agaagctcgc tcaaatgatc
      721 caagaacgta ttaaagaaga gttaggtttg attgctacgg taggcgtcgg cgataatccc
      781 cttttggcta aattagcctt agacaatgaa gcaaagcata atgaaggatt tattgcggaa
      841 tggacgtacg aaaacgttcc tgagaaagta tggaatattc ccgaaatgac ggacttttgg
      901 ggaattggtt ctcgaatgaa aaaaaggctc aaccaaatgg gaattttgag cattagagac
      961 cttgctaact ggaatcctta tacgattaag aatagattag gtgtgattgg attacagctg
     1021 tacttccatg ctaacggaat cgatcgaaca gatatagcaa tcccgccaga accgacaaag
     1081 gaaaaatctt atggtaattc acaagtgtta ccacgagatt atactagaag aaatgaaatc
     1141 gagctggtgg taaaagaaat ggcggaacaa gtagccattc gtatcaggca gcataattgt
     1201 aaaacaggat gtgttcattt aaacattggt acgtcgatct tagaaacgag accaggattt
     1261 tcacaccaga tgaaaatacc tatcacagat aatacaaagg aattgcagaa ttattgtctt
     1321 ttcttgtttg ataagtatta tgaaggacaa gaagtaaggc acgttggaat aacctatagc
     1381 aagcttgtgt acaccgattc gcttcagctg gatttattta gtgatccaca aaagcaaatt
     1441 aacgaagaaa atttagataa aattattgac aagattcggc aaaaatatgg ttttacgtcg
     1501 attgtacatg cttcttcaat gttagaaagc gctagaagca ttactagaag cacacttgtt
     1561 ggtggtcatg cgggcggaaa tgggggaatt aaaaatgatt gattacaatg atcgtggacg
     1621 tttaaagtgg gcagggtttt atttatctga tcatactgaa gaaattgatg ccgacaatga
     1681 acaacgaacc agtcaaaatt tagcaaaaga acaaatgacg actgaagaga ttgcggaagt
     1741 attgaacgct gcagtcataa aaaatagatc agtatctatt caaaaagaag aacgaaacgc
     1801 tgaaggatac tatccagaag atattgttgg caagatctta ggtagtgatg agctggggat
     1861 ctacgttgga aaagacaaaa ttcactacga cgaaatacgt catatcgaat tttaccagga
     1921 taataaatgg agtcgattag aatgaacatt atccagcaat atgagctaaa gtatataacc
     1981 tttgatcagc tatcagaaaa gatttggggt tatggccaac gtttgataaa tgaggttggt
     2041 gttgagcgct tctctttcta tgtagaagct gcagcaggtt atcataattt taggttttat
     2101 attttcccgt tatatatata aggtatttta cggattctat agagttatct ctatgaatcc
     2161 ttttttgttt ttacaaatag gagtagatac tagaataatg cgtgtgttaa actattattg
     2221 caaacgcaat aataaaagaa agtaggctta cgcaccatgt ctatcaatac atttgtttac
     2281 tcgcacccga tcaatgttta cattatcaag aatttaggaa tcactgtaga acaattttgt
     2341 gagttatatg cctacccaca aggaacggtt gcaagctgga taacccgaca acgtagaata
     2401 aaatcattac ctgcttcatt cgtttatgat ttaagcctgg cctctagttt aaacatgtct
     2461 gatgtttatg aaaagctcct gtctttagaa aaagaatatg attccttcaa gattaaacat
     2521 gataaaaaaa taaaaaaaca tatataatat caatcacaat tgtataatat tacgatatct
     2581 taatatcatg atatcgtaat attgtttttt tctaagaaaa tgcactataa gaagggaatt
     2641 attagtaaca atatagacag tttaataatg tttaaattat tctagttgct gctcgcaaaa
     2701 catatttctt atgattaata aaaaatttta aagtaataaa aagtgattat tttactttcg
     2761 aataacatat gaataatctt tatatccgaa ccgatatata tctttaaaac aaaataatca
     2821 cacaatatta caatataatg catatacgat attgcaatat cacaatatta tgatataatt
     2881 atattataat ataagatgaa tgggggatat tttaatggca agaatattgt cagtagccaa
     2941 tcaaaaaggt ggagtttcta aaactacgac tagcgttaat ttagcagcat gtttgaaatt
     3001 tgtttacggt aaaaatgtac ttttagtgga cattgatcct cagggaaatg ctacagataa
     3061 tgtaggtttc gatatagatg gaacaaacca acctacaatt tatgaagtat taaaagggga
     3121 ggctgacatt acagatgcta ttctggacta taaaggaatt gatgttttgc cagccgacat
     3181 agccctttct tctgcagaga gggaatttac tcaagtagga agtgaacaca ggcttaaaag
     3241 agtacttcaa ccaatcaagg acaactatga ttatattata attgattgtc caccaagttt
     3301 aggaatgctt acagtcaacg catttactgt ttctgatgaa attatcattc cagttgaagc
     3361 tgcgtatttc tcattaaaag gactaataaa gttaagtgaa actattgaaa cggtaaaaga
     3421 atatacgaac gacaagcttg aagtacgagg agtcctattt actaagtata ataagcgtta
     3481 taacatttct aaagaaatga cgactgcagc aaccgaaatt tctagagtca ttggtgcaaa
     3541 aattttcaaa acttatattc gtagaacaat aaccgttgac gaagcgcaag ctgctggatc
     3601 agatctaatc aattttaata aaccctcaac agctgaagat gactataaag catttacaga
     3661 agaatattta aaagaagttg gtgattttga tggcagaaga taatcgcagt caagctgcag
     3721 taagtagact actaggaaat aaaaataaaa agaactacaa accaaaagaa gtagaagaaa
     3781 tcgaattaac caagaaaaat ataagtaaag tcataaaaga tcaaggaagt agtgtaagaa
     3841 cagcagatag cattcgtata aatccaattc taatgtcagc actcaaatat tggacgacta
     3901 ttgctgaatc agacaaaagt aaaccagacg ttatagaaga tgcgctatta aaatccatac
     3961 ctgaagaata cttgattgaa ggatataaac tagcaaagaa acaaaataaa atttaattta
     4021 cattcaatat cacgatatca taatatcgtg atattttttt tataaaaatc agtagtatca
     4081 aagctgataa gtcgcttatc ttcatattgt aatattacga tatcgtgata taatgttatt
     4141 gtaatatcgt gatatagtta ccatgagctt aaaccctgaa atagagcggt ttgctttaat
     4201 ttatttttta aggtaaacta aaaaaagata aaaaaagact actgctttaa caaagtttgg
     4261 cgacttatca gcagtagaaa aatatatagt ggatagatat atttgtttaa ttcattgtaa
     4321 catagttttg aacaaaacgc tatcctacag aaagaggatt ggctatgaat gattttaatt
     4381 attacaaatc aaaagaaatt tatcgtgaaa aatattatca aatgcctaag gtatttttta
     4441 ctaacgaaaa atacatggat ctttcaaatg atgcaaaaat tgcttatatg ctacttaaag
     4501 atcgatttga ttattcagtc aaaaataact gggtagattc tgatgataat atattcttca
     4561 tattcacagt tgaagaatta atgaaattgt tacaatgccg cgaaggtaaa gtatctaaga
     4621 tcaaaaaaga attagaaaaa gcaggtcttc tgaaacagaa gagaggaaga gtaaataaac
     4681 gtgatggtaa gattgaatct atgcctaatt tattgtactt aggcaagcca gaagttacga
     4741 atcaagacgt ctttaaaatc atggaagagg aagacaatac tgattccacc gtaattgcga
     4801 aaatcgcaaa tacggcaaaa ccgcgaaaaa tcaatgattc taccgtaatt gcgaaaatcg
     4861 caaatacggc aaaatccagt catatcaagg actcaaccgt aattgcgaaa atcgcagaca
     4921 atctattcta ttctaattct atagacacta atagacacat aatagacact gaaactgacc
     4981 agttacaaaa tcaagttttg ctagataact tcgtcgacat catgcaagag gacagtataa
     5041 atacatttgt accagaaaat gtattaaact tgataaaaac attttcttca agttacagtg
     5101 aagctcaaca aaccgtccaa actattcata atgcaaagaa aaaagctgaa gaattagagc
     5161 aaactattgt cgtttatgaa gaactggaat cttatggaat tgatgctgac aaaggattat
     5221 atatgacttt actgaaagct tatcaaaaac aaaaaactga aaaagtaaac aatttgcaga
     5281 atctaatttt cgtttatgta aaaaattggt ttgtcgaaaa agctattgcg gcaaagcttg
     5341 caaacgaaca aagagaaaca tttaacgaat taccaacagt tagtacggaa aattggttag
     5401 agtagttgat aaaaagcaat tactataaaa atgatcaatt ttaaattaga acttttttat
     5461 gattttaatt ttattacaaa tattacaaat aaatagttag tattccggag tttttagatt
     5521 aaaagtttat gaaaacaatc acaatcgtag gttttaaatt cataatcgtt agatatttta
     5581 gttatttttc actcaaaaaa atagggaact agtgtttgca tatgtgcttt tttgagttat
     5641 aataattcat gaagagaggt aattcactta tttccccaag ctgaaaagcc tgatattact
     5701 tcttttcagg cagtgctgct aatattcaat tacacttttt tgtatatttt tagttgtttt
     5761 aaatttaatt gtctgctatg cttgtgatga attgattttc gttctgcact tttggacgat
     5821 aaatttggac aattgaaaat acaaaaaaaa gaaaggccct gtgagactcg gaaggctggt
     5881 aaccttcgtc gacgtccaca ggtggcatta agatcactcc cttaatgcgt ttacctttca
     5941 gtgttacggt attacccgta acggatttag tataacagat attcaaaagt gataccactt
     6001 tttttggctg tgttaaggtt aaaaatacgt gatattacga tgaatctatg cctaagcata
     6061 atgttataaa aatgtccgtg tggctctttg ccgtatgacg aacgctgaaa ttagagggaa
     6121 cgcatgtttg gatgcgttcc ctcttttttg tttgtcatga acgaaaatca attctctatt
     6181 tttgaaaaat aacaagaaaa acttgttaaa aatatttaaa gtaagtgggt ggattatttt
     6241 atgtcatata caggaatttt gagtctcgaa gatatttgtc actacggtaa acggtgtacc
     6301 gctacagaaa agatcacgaa gaaactatca actggtcaaa gtaaaaccgt tgtgcagtgt
     6361 aagaaatata taatccagaa agacaaagtc tcagaagaaa tgatttatta cattgggaaa
     6421 caaaaacaaa taattttaaa agatcctatc cctttaaaag agctatatcc aacaatcaaa
     6481 catgtttatg accaaaacgg tgtactgatt ggtcgaagaa aaaatggagt cttgcgttgt
     6541 accgcaaaag ggatggggcg tttaatcagc tgaattgatc attctcataa cttgtatttt
     6601 atcgtttttc gcttgcttga aaatattgtc gagacttcta aaggaactta gaatcactag
     6661 aaaaataagg agtagacttg atctatcaac aattcctttc ggggggtgac ggggtaggtt
     6721 tttacgattg gtaacgtaaa aaattggtca atcgagtaaa tgcccttgtt tggatagtgg
     6781 tgaaatgaat ttgtgtaaat aatatgcaca gagtaaacaa gagggaattt agaagttaga
     6841 caagttagaa tagcgcctag attaatcatg agcagctact aagggatcta ggctttgacg
     6901 caaactcatg gaataaggag taggtcttct cgtaatgggg aggacgcaca agaaattgtg
     6961 tcgtgtgctg cagtacatgt agatacttgc gaagacgttg atctttggat caatcgagca
     7021 tttaccgttt tctactaggg tttctcgttt cggacgccat aaacgagtcg atttatttta
     7081 tatatttttg agtgaacgaa aaacgataaa aatattcgtt atgaagtaaa actaatttag
     7141 tagatagcaa aggtggtaga ggaatgattc aaaaatttgc gaaaatgagt aaaactgcat
     7201 ggattgaaaa gcttgaaaat gacttgagtg tcatgcgatt agctccaaag aaattcaaac
     7261 gagagattgc agaaaaagaa gcaattttgt cggtattgaa tgcggtagac gataaatgtc
     7321 cagtaaaaat tagtttttca gatatagcaa caattatcga aggtatttat tatggtgaag
     7381 ttgatgaaga gatatcttct acaaaagatt tattagattt tttggataaa aacaaagata
     7441 cctttatagt actaactgat tatgaggagg ttacaacaaa tgaaaaatta tgatccgaat
     7501 agccgatggg gaacacatac gattaaggtt tctttccaac gttgggatta caaaggattt
     7561 gtgacgttta gaagaggcgg aaattgtaaa ggattagatg ttttagcatt agacgaggat
     7621 gatttatacg atcaaaaatt gactgataat ccaattggat tcggcttgct acctgaagat
     7681 gatcaaggta atgaatggtt taaaatgacg cttatgaatg ataatggaga cgaattatct
     7741 gttgaagata cttggtcata tttgagtgat tatattgtca gcgttgaaat cattgaattt
     7801 gtagcggata aagaagaata aaaactggag gaataaattt tacagataaa ggcttagaag
     7861 gagtcacgac gattaataaa aaggtagtat taccttaggc attgagtaag gaatttattt
     7921 aggaggaaaa ttagatggcg cgaccagttt tagaggcgat ggatatggat actttgacaa
     7981 aaggaaggta tgtacagctg ttagctcgtg gttattcacc taaagaagta ttcaaatccc
     8041 tgtctaaagg aacagatatg gaaaaagaaa gattacgaaa agaattcgat tattggagaa
     8101 cccataatgg aatcaaggat ttgaaaccag cacggccagt aattaaacga aagaaaaaga
     8161 taacccaaga ctgaagtgta gagcgtatgt gatcataggc ttttttattt ttgcaaaaaa
     8221 caaggaaggt aggaaaactc atgaattatg aagaaatcga aaacagaaaa aaagtgtcga
     8281 aagaaatgga agaaaagttg ctaaaaacga tgaaacaaaa acaccttaaa agattatctg
     8341 tcatgcaata tatcaatgat atgcagataa caggaaaaga gaaagcttgt ttgttaggtt
     8401 caatgaaaaa ctttgaacag ttgagacgaa cttatgtgaa aaaaagttct aattgtcaac
     8461 tattgttaga agtctcatga cattcattaa tagaattcat ttttaaaact ttttctatgg
     8521 aaatgagaaa aagatttttt gttttgtctg aaagggggta gctggatgag aaaatggtta
     8581 tttggttttt taggagtcgc tttaatcgtc ttttgcagtg tgtttggcta tgttagctac
     8641 caaaaacatg aaggaaaggt gtttaagcaa aatatagaaa aaaagatgcc cgtagatcaa
     8701 atcaatgaac atgcaaagag ttataaggag gatgcaacga acgtcaataa tgatatgtct
     8761 ttggggcaaa tgttgtccat acaaaaagaa gcaatcgaaa tgggtgtcaa taaacaagcc
     8821 tttgcacaaa ttcagatacc tgcacttggt ttagcattgc caatttttaa aggggcaaat
     8881 caatacactt tgagtcttgg agcggctact tatttttatg aagatgccga aatgggcaaa
     8941 ggaaactatg tcctagcagg tcataacatg gaaatgccag gtgttttatt ttctgatatt
     9001 caaaagttgt cattagggga ggtgatggac ttagttagta acgatggcgt ctatcgttac
     9061 aaggtaacgc gtaaatttat cgtacctgag tactttaaat taatcgatgg cgttcctgaa
     9121 gagaactctt tcttgtcttt accaaagaaa ggagagaaac ccttattaac gttgtttact
     9181 tgtgtttata cgtcacaagg aaaagaaaga tacgtggtac agggagagct acaataacaa
     9241 ggttttaatc aacgcgagtc gttgaaatat gaaatgagga gagtaaaaaa tggcaataaa
     9301 tcggtatgta aaaaaagctg gaatgcttgt tggaatgtta ggaatcattg caagctgctt
     9361 tcaggggtat caagtacacg cagaagatgt cacgcaaaaa acaccaccag agaaggtcaa
     9421 tatcacggtt cataaattaa tgtacgatca aggaacgcaa ttgaatgttg atattgacgg
     9481 gatcaaaaac gatggttata cgcacgatca atatcctaca ggtgtaacga aatacaacaa
     9541 ggcagattat ggtgatgttg agtttacgtt aggaaatatt acggatcaag tattaccgag
     9601 agaagattct gatctaacca acgcgaaagt ggacgagatt gtacaagatg tagaagacaa
     9661 gggatctaac agtgagtacg tcaagaatgc aaccaacaaa attacttcag ctgtagatga
     9721 aaatggtgag atcactttta ctgatcaacc agcctatgtc aattccaaag ggaatgtata
     9781 tgtggtttat gaaagtaaat ccgctgctgg attagtcaca caaaaagcaa aaccaatggt
     9841 tgtgattgcg ccaatgacag ataaaacagg atcaggattt ttaaaaaata ttcatttgta
     9901 tcctaagaac attgtttcta agttgagttt tgaattaacg aaatttggtg acgatggcgc
     9961 agcccaaagc aaacaaaccc ttttaaaagg ggcaaaattt gagttataca agggcgaacc
    10021 tggaaaagga acaaaattgg gtgatcttgt atcggatgat caaggaaagc tgactgcgac
    10081 tgatttgact ttaggaaaat actattttgt ggaagttcct tcggaagttg tagtgggatc
    10141 agatcaagaa ccaacagcag atcaatattt attaggcgca gacgctcgca acgatgcgca
    10201 taataaatta acctttgaaa ttaccaatga cggcgtgaca agtgacttaa aagcttccta
    10261 cgtgaactat aaagcaccag tgatcgataa aacagtgaca aatggaacag gacaagaaca
    10321 ttctttccaa attggtgacg cagtgaatta tcaaggaacg attcatatcc ctacagatat
    10381 tgcaggtgga acagatggga ttacagtaaa tggtgtgaag agtgaaactt ctccttacag
    10441 cgtatttaag tggggagata cagcaggaca aggactttct tatgtcgctg ctaaagcaaa
    10501 tatcaaagta acaaataaag atggatctgt ggtattaaaa gaaaatacag attataaaat
    10561 ccaaaattca gaaaatggct ttgtgatcga ttttatcgtg aataatggcc aagtaagtga
    10621 tactgtagca agtttacacg gacaagatct tcaaatgact tataacatgt atgtaaatga
    10681 cagcgcagct gttgccaatc cattaacaaa tagcgtggat ttcgtttata acaacaatcc
    10741 atttaatcag gaagaacacc acgaaaaaac gaaagctgac gttgtgactt atggtgcaaa
    10801 attcttaaaa gtcgatagcg gactatttgg aacaggaatc aaagcaacac cgcttgaagg
    10861 cgcagaattt gctgctaaaa acgcagaagg aaaatattat ggtggattag ttgatacgga
    10921 taaagacggt gtcaaagaag cagtatgggt agacgatgta gcaaatgcag cgatcctaaa
    10981 atcagataaa gaaggtcact ttgaaatcac tggtttaact gaaggtgaat attcactgga
    11041 agaaacaaaa gcaccaactg gttaccaaaa attaacagaa gatattgcat ttaaagtaaa
    11101 caaaaactca tttaaagagg aaaatcgaat cactgttaaa aataatcaaa aagcagctat
    11161 tccattaaca ggtagtaatg gtttccaaac gtatgtattg atctcttgct tgttgctagg
    11221 agcaggggca ctttctgcag ttgtttactt taagaagaaa gcctagaagg atcagctaat
    11281 gattattcgt aagcccccaa aaagaagagt catgcacaaa ctgatgatat ttttttggtt
    11341 gctgtcggca gtagcagtct atttaattcc agctgtggaa acaaatgtag cgaaatttca
    11401 acaacagcaa agaattttag ccattgaaaa tcttgaaaaa acagattctt ctttggatgt
    11461 tgcgaaaata gagttaggcg atcctgttgg tgttcttaca attccaagta ttcctttaaa
    11521 actaccaatt tatgacggaa cttccgacaa aattttagag aatggtgtag ggattacgga
    11581 aggaactggc gatattacag gaggaaacgg taaaaatcct ttaatcgcgg ggcacagtgg
    11641 actttataaa gataatcttt tcgatgattt gccttcagta aaaaaagggc aaaaattctt
    11701 tattaaggta aatgaagaac aacatgccta tcaaatttat cgaattgaag aagttaaagc
    11761 agaagaatta caaaaaaatt atttagatta tctcatgcct gaaaaagacc aagataggat
    11821 cacgttaatg acatgcacgc caaaaggtgt taactcacac cgctttttag tgtatggtga
    11881 acgagtgtca ttcagtaaag aagatttaaa actacagaaa aaagcaaaaa aataggaaat
    11941 caatttcatt ggattggcga ttagcactgc tattcgcttt ttttgcgttc ctattatttt
    12001 tatttataaa aaaataagga ggagatcgga atgaataaga gaaaatggct gtttgtttgt
    12061 gtagcagctc tctttgcaac tgcagcttac tcacagatat cttttgctga tagcagtact
    12121 actccaacta ccagtcagac aacagatagt tcaacaactg caacaagtga cacggacagt
    12181 tcgaagaaca ctgcagaaga aacggcagat tcaagtacga gttctactga atcagcggaa
    12241 tcaacgtcag aaagttcttc agagacttca gaaaaactgc aagaagtaga agatacgcaa
    12301 gtaatcaagc aaaacattca tattcaaaaa attatttctt cgaatcgaat caacaatcaa
    12361 ggaaggcaag tgactcagca agaaggtgtc aatggtgcaa agtttattgt ctacgatatc
    12421 acgaatatct tagaagaaat gacacaagat gataatgaga aaaatcaatc ggtagaaata
    12481 attgagtcaa aattgaaaga tcgagcaaaa aaactttcat acgatcagtt gaaaaaagtg
    12541 actgaaggag aaacccaaac aattgatgat caggaaggcg tgattgattt ttcaattgaa
    12601 gtacctgcaa acaaaaaaca agcatactat atcgtcaatg aatcaagccc tgaaaacatt
    12661 tcaaatagcg aagatattgt tttattgaca cctgtttcag atgaaaaggg tgaatttttg
    12721 acggatgttt ggttatatcc taaatcaaag aaatcagagc cgaaagaaga aattaaaaaa
    12781 gttgtatcaa ctggtgtaaa gaaaaacttt tttgataatt gttgggattt tttcactcat
    12841 ttatttttta acgattagga tgaaatgatg aaaataataa aaatgttagt ttttaatcta
    12901 tcttttttag ccgcaacaat attgtgttgc ggtttagttt tttctcaatt atcaatttac
    12961 ccaagatatc aacgattgtt cgttcatttt ctaaatatcc agcatgcttc catgccacta
    13021 gaagtgatta acaagctctt aatcaatcca atttttatgg ctagtttgtt tcttcttgca
    13081 ggattggtaa cgtgggtatt aggaaaaaaa gaatgagaag cttacaatcg tttaacaaaa
    13141 taatagatgg agggcattat ggaaaataaa attcattgca ctacgggaag agtatttact
    13201 ttagtgttgt ttttgttcct tcttcttttt gcaaggggaa ctgcagtcca ggccttagaa
    13261 aaagttgatc cgagtcaaat caaagaaaat aataatctgg tactttatca tgatagtgat
    13321 tcattgagaa atatcgaagt aactgggatc aatggattta agatactaga tgagtattca
    13381 actaaaaaac aaaaagtact tgcttggcca aaagtcaaag atttgaaaag cacgtcaaaa
    13441 attgttgccc attatcaaaa ggtgggcact tatctaggaa aagaaattga ttgcgacgta
    13501 actttttcta attttgtagc gcacgctggg gttaaccaag acggattagc taaaacaatt
    13561 aatgggaaaa agtattataa ttatttgctc tttgataaaa acatttatcg aggctttttc
    13621 atgcagtcaa tcaacgttat gaatgttaaa ctagatttta agtatgcagg atcaaaaaca
    13681 aaagtagctt tgagtgaacc acagccagaa attaattcaa aaatgagatc gatgagagca
    13741 gctggtattg agttgaatga tacatttcta tctatttaca gtttaaatgg acagtatcag
    13801 caacgtgtgt cttggtataa tgacaataat gaatctgtcg gtgaacgtaa tattgatatg
    13861 agagaatttg ttgggtacga aaaaatggat cgcttacctt attttgtcac aacagataca
    13921 gcatgtgcag aatacaaagc tcctgcgtta tcaacaaaca atttaacttc aaaagtagtg
    13981 ggaggacgtg cagaaaaggc ttatagctcg aatgatcatt tcaccgatgt tgtaggagct
    14041 gatacttatc acagaagtgg tgtaacgtat acgcttcaag gcgcttcccc aacattcatg
    14101 attggcgcaa atacgaatag tatgatgttt agctttgata ctgcattgct ttggacacca
    14161 caaccatcga agcctacaaa agaagtgttt aacaaagcta atactgaaga ggcagcacac
    14221 aatattgaca aaaaagtgat tccacaagga tcagatgttt actatcatat tcaccaaaag
    14281 tttgatgcat taacagtcaa cacaatgaac aaatacaaat catttaaaat cactgatacc
    14341 tttgacagca aaaattttga tatggtatcg gatgggaaaa actatgatgg cgcattgtgg
    14401 tatgttgaag cgaatgggac ttggaaaaaa gctgatacag gaggaaaatt aactgtatca
    14461 ggtaatacag tcacttatac tgcttctgac agctttttag ctaatgctgc aaattacggt
    14521 agggactaca ttttgcagct ccatatgaga gcaaaaaatg atgcaaaagg aattgcacca
    14581 aatgtggcta ctgtaacgtt taacaatgat ccgaacaaag gaacacaaaa aacaaataaa
    14641 gtagaaaact atttctctct taaaccaaat aaaaaagtgg aacaagatgg gaaagatgtt
    14701 gatggtcgaa atgatggtaa aaaaggaacg cctacagctc ctttaaatgc aggatctgaa
    14761 gtacaatatc tagtcacaca aaaatggcat acaaaaggtg tagacattgc gctcgatcat
    14821 tacaagcaat ttagtattca agatccagtg gaagctcgtt tgacgtataa agaaggttct
    14881 gcgcgagtga tagatatgag tacaggaaaa gatattactt cagaaggtat actaacctat
    14941 gatagccatt caagaacgct gaaatgggaa gcttccgctg atttcttaaa taacaatctt
    15001 ttagatggac gagaaattca actgatattt acagctaaaa ctcctttgaa tgaagaattg
    15061 gatatcgaca accaagcaaa agtttcagta gaaagtattg agaaagcaac taatgtagta
    15121 acgattgggg taaaacctaa cttacctcag gtcattgtgc caaaaactgg atcgaatcat
    15181 ttagtgctaa ctgttattgg aagtatatta tttcttactt tagcatcatt gggctatgta
    15241 gttttaaaac tcaaataaat ataaaaacaa ggctgatttt cagccttgtt ttcttttatt
    15301 tgaagattgg attcgcacta ataaatacat tatctacgcc aattctacca gcagagtcaa
    15361 ctacaaaatt atgacctaat ccgagaatac tttggttatt ataaagtaaa tttgcattat
    15421 gggcagggct atttttccat cctaccatgg catcgatagc accatcgtct gataaaaggt
    15481 catcaatata cagcccagtt tgacttatgt tttctccctt agcaccatag cagcctaagc
    15541 tttccaataa atcttggaaa gtaccaccac cagggcgata gtgactgaag ctgacttgta
    15601 attctttagc tcgaatatct gcagcctgtt gaacaatatc attgaattgc attggggcaa
    15661 caccattttg ttggcgataa tcatttaacc aagcaagttc tttttgacgc ataaagttac
    15721 gtaattcgtc taatgaaatc tttttatatt tgaatgtgat tgttttattt tgatcgacgt
    15781 tattaatgga tcgttggccg tttttatcca cggtaacggt aggatcgtca tttctgcaca
    15841 agaaataatt ttgatcagtc atatttttcc aaggagtaga gaagctatcc ccttcgtata
    15901 ctgtttttga ttcactagag agaacagcgt tggtatcagc atttacatga ttaacagtca
    15961 cattaaattt acgagtgtag ttgaaaatat aattttgact ttcagtcaca ttgtttaagg
    16021 ttactgaatt tgttggttct acccgatcgt ctaaaacata tccttgaata gaaggagcag
    16081 ttaaagtgac actttcaccg tgagtgacgt tgattatctg ttctttcaaa atatcaactc
    16141 gcccctcatt tttgtagttg ttattgcaca acaaattgat ttgatgcact tgattataat
    16201 tgaatgtgat tgttgtatta cctgtgattg ttttgctttg agtatcatta ccaatcactt
    16261 tgtagctaaa attatcttta gcaccttcgc tatatttaaa tgcttttgcc ttagcagtgt
    16321 aagtagaacc agaagtcact tttgcttgat ctgtagcaat cacttgacct gtttgattgt
    16381 caatatggtt aatggtaacc atatattctt ttggcgtttc aggtttgtgt tgcaaccctt
    16441 taaatgcagc atcaagacta tttttagcat tgtttacttg tgtttgagaa accttagaat
    16501 cgtctagtac ttttttagca ttggataaag cagtctgaaa attattccaa gattcttctg
    16561 taaaatctcc tttagcagtt gattttactc ggttgtataa agcttgtaat tcagctttat
    16621 tcacagtagg aactggtttt ttctttaatc ctttgtaagc agaatctaat ttatttttgg
    16681 cactatccac ttgcttttga gtcgcttttg agtcatttaa cactttctta gcattgttta
    16741 aagcggtttg gaatgcgttc caggtattgt cggtatagtc accttttttc gttcccttaa
    16801 cgctcgtata aagagcttct agagccgatt tattcacttt gggtttagag gtgtcagcgc
    16861 cataccaacc gataccttcg tatttccagc catagtcttt ggttaaccat tgtacttctt
    16921 tccaacttgc agtgtagtga tgtgagccag attttacacc aggaaggaaa aggcggtaga
    16981 tcggtttatt atttttttcg gcagaacgcc aggcaggccc ttcataagtc cagccgtaat
    17041 ttttagtcaa ccatttcact tcatcccaac tagctgtgta atggtgatca cgaagacctt
    17101 tattgtacaa acgatagaca agatggccac cgctagtcgg tgcttcccag gcggggcctt
    17161 cataatttcc ccattttatc tttactagat ggtcacgttc ttttttgcta gaggtgtaaa
    17221 aatgttcatt attatttgga ttgtaaaggc gatacatgac gttagttgaa gcagcgtcaa
    17281 cagatccagc aagactaagt gaggcagcaa atgttccaat agctaaaaca gtttttggat
    17341 taaataattt cattttttca actcctaaat ttaattaaaa atgtttacat gattattata
    17401 tatcggttaa ccgatatagt caataaaaaa taagaaaagc cttatataat tttggctttt
    17461 cttattttat ttgttagttg aagtattaat atcaacgatt tcttcagcca atttatttaa
    17521 tttcatagca tttatgaatc tcatgttgtc tatactagag cgtttgttgg caaggtcact
    17581 tactgtagtt aagggtacgc cagattctat ggatatagaa cgaactgatt taccactatc
    17641 aaacagcaac cattcgattt tctttttatc agcgtacatt tcaatgctcc ttcttgtttt
    17701 ttctttatta tagctctttt ttgtaatgaa tcacaatatc aagagaaaaa ttttctatag
    17761 aaaggtggat ttttatgagt attcgttctc cgtgtatcca aatgaggttt gttgctactt
    17821 tcgttatttg atatactaaa aataaacaac gaaagaagga ataaaatgaa gaaaaagcaa
    17881 ctatggtttg tctctatgtt tgcaatattt tttattattt taagtggatg ttcaagtaat
    17941 aagaaatcgg aagctaaaga agataatagt ttattgcaac caggaactgt ttggaaagaa
    18001 gaagtaggtg gtcttattca taatttaaaa attatagatg atactacctg ggaatattct
    18061 gaaagtgtat ggcatccaga tcctgtacaa ataacagtta agaggcaaaa agactatgaa
    18121 ggtttagaaa gatacaaaat agtagattct gcaggcatta gggaatttat aaacaaacct
    18181 gattcattat ttattgttgt tccctatgag aaggatggcg taaaaaaaat aatttttctt
    18241 ggatctagca aggatgagaa gcgaacgaaa gagaaattaa ttcatgatgg atcacaatca
    18301 aataaataca aattacaaaa gactagcgag tgattgctag tctttttatt tgtcctgaag
    18361 gaggaatcac agtggcaaaa gctaaaacgc catttgaaaa agccgcgtct aaaatcttag
    18421 aacgccgcgg tgtatctgaa acacagtgga aacaagaagt ccttgaaaaa gcacaatttg
    18481 acctattacg gggaaaagac aaggacttag aaggatatat ctatcaacgt gaacgtgaaa
    18541 agcttgtttt cgatgaaatt attaaacact aaaaggagcg aacaatttta tgtttgaatc
    18601 aattacaatg aattttttaa cacaaccgaa agtagtagct gcggttgata tttttagagc
    18661 attacaaact gccgcaacta gtttgaaaaa taatctgatg atttttgctg ctgttgcggc
    18721 agtggcttcc attgctcttg gaggtatttt cttcttcttt gggcgaagag gagctgaaaa
    18781 cgggaagaca cacgtaggtt atgtattgtt gggggtagga gttattttag ctgccgcttc
    18841 tattgtagtt tcaatgttct caatgtttgg acaaacccca caaggactat aaaaaaacaa
    18901 gtagaaggag taaaaagtac gtggctactt tttactcctt tttttatgga aggagaatat
    18961 tcatgttttt tacgatttca ggcaatgcaa aattggataa agagttaata agaaaagcaa
    19021 cgaagaaagt gggaaagcgt acttcctttt taccgaaaac aattaaatat tctgtcattg
    19081 aaacggaaga acagctacat gtgttaggac aagtgttaga acaagagaat caatcttctg
    19141 ttaaattgac tctcacgtta catgataaag aaggaaaaat agaaaaggct ggcacagaat
    19201 acgtttttgg ctcactggat tatcaaattt cagaaggttt ttatcctaca ttgattgatt
    19261 taatccgcct agatatattt aaagatacgg atgaaaccat gagtgtggaa gagtcttatc
    19321 gaaacgaaaa aattttacaa gttttagaag aagagttaga aagcgaccaa agaaaagtga
    19381 atcaagtgag tgaagtcgaa gcaaacgaag aagtaccatg ggaacgccca atctttccaa
    19441 aggatgaatc aaaagaaaaa gagaaaggaa ttattgacga acaagctgac attttgactc
    19501 ataaatcaat atttgaggaa gagaacgagg taatgggtca attggaggta gatccctttg
    19561 aagaaacaat ggaggatgaa gagacggccg aagtagaagt tgatccagtt gaagctaatg
    19621 aagacccaac atggactgaa ggggattctc aggaatatat tgaaccactt atcagtgaag
    19681 aagtagagaa aaatgaaaca gagatacaaa aggcaacggt atttgattat attttggatc
    19741 aatatccgcc agagcatgaa tggatgaaag aaaaaaataa attattttta aatgaacttt
    19801 attcgaaata tcaattacct caaacctatc aagcattttt agagagtaaa gaagaattaa
    19861 tctcacaggg taaaggcaca cttttagaac agttagagtc tgtccaatca acaaattggc
    19921 atgagcttgc acaagaagat ttattagcta cttttgaaga acgtcaacaa gaaacagatc
    19981 aagaaattaa tgagtatgcg acggaacagt cgtcgaattg ggagaaagca aaagaggaac
    20041 taaataaaga agaacagcga gtgattgaag aagaagtaca aaaaattaaa gagcggtacg
    20101 aaaagaaacg tgacaatgcg tattccttgt gtgtagaaaa gatcaacaat tttactcaga
    20161 cgaatcaaga agcattatcg aaagaaaaag aaaacttatt aaaagaacgt ataaaagaac
    20221 gaaaagaaga attttaccga gggttgcgta ctgataagct aaggattagc aaagaattaa
    20281 atgaacaact gaataaactc tatcaaacta cgactgaagt tctgatggaa aaacacgagc
    20341 agatccaaca agaattacaa gcacaaattc caaaatggaa aaaagagtat gaaattcacc
    20401 aacaagaatt gaaagaacaa gaagaaaaac aacggaaaaa agaaaaagag cgcgaacaat
    20461 tagaactaaa aaaacgtgaa caggatcttg cggaacaaaa aatggcgata gaaaaacaac
    20521 ggctagaaca agaaaaggaa aaagaagcag ctgctcgtaa ggaacgtcaa gaacaaatgg
    20581 atacattgcg acaagcacag ttaaattatc tgacacatcc tcaaccagta gctgctgttc
    20641 aaacgccagt gaacaaccaa gcaaatttaa ctccttcacc ccagcgttcc tttaacggat
    20701 ggatggtggg atgtattgca agtttaagct tgcttctagg tggaggaaca gtatttgctt
    20761 ttaatcatat caatcaatct tcacaggctg attctgtagc cacaacggcg tcaatcaagg
    20821 aagaagccca ggaacaagca agaaaagagt atgaacaaca attagaagaa ttattgaatc
    20881 gtaccaatca gtcaacaaca gagtcaagca tggaaaatag caaagagagt gattctagta
    20941 aggctaaaga aggatcaagc gattcaactg tacaatcaaa cacttccgaa agttcagaat
    21001 caaatgaggt gaaataatgc gtgttagaga aacaacgaag cgaaaagaca aacgattcaa
    21061 tttagaaaag aataggtatc tttctcattt ctatcgctat actccctcag gagaagagct
    21121 accagaaaaa catgacaaac gcatttcgaa aaagattttt tatagtggac ttgtatacct
    21181 ccctttagag tatttagtaa caagagtacc agaagttttc aaccgattgg aaattgttaa
    21241 gggaagctta caaggaataa atctgtttca attacaggaa atgccacagg tctttcattt
    21301 tgattcagct ggtttttgga atccatttac tcctacaact agtttgggta cacgtttagc
    21361 aatttcgaca gtttgtgtgg ttccagctgt acttatggga ttgaaaatgg acatgtggtt
    21421 tcgtcctcta gcagatggac aaaaagggga taatcgatta atgaccttaa aagaaatcca
    21481 acagatttat ccgcaaatac ctgaaacaaa aactactttt ccaggttatg gaggtattcc
    21541 aatcacacat tacaaaaaat attggtacat tcaaacagat actgttaata cgtgtattgt
    21601 tggtacctct cgatcaggga aaggtcagat tgaagtgtta gccaccattg acaatttatc
    21661 tcgtgcagaa aaacaaagtt caatggtcgt taatgatcca aaaacagaat tatatgttgg
    21721 tagtaaagat gaactagaag ctagagggta tgatgtatat gcctttaata tcaccgatcc
    21781 actgcaatca atgagtgata atccattggc actcatcgtg aaatactgga aacgaggaga
    21841 catagatact gcaacacagt taaccaatac tttcacaaat acgatttatt atgatgccaa
    21901 cgcttcagat aacaaatact ttaacgaaaa cgcgcagaaa gctgtcaatg cgcttatttt
    21961 tgcgttgtta gaacaagctg atcaaacagg agattactcc aaattgacac cgaataatct
    22021 cgttgagctt ctttctgaga ttggcggttt taattattat ccagatccag acaatgaatt
    22081 caaacagttg aatgccttag atgaatttat gaatcagttg ccacctggaa acattgcaaa
    22141 aaaacaatat ggggcaacga aaattggatc tgataaagcg aagggaaata ttttatcaac
    22201 ggcaatcacg ggattgaatc catttactct aacgaaaatt gcaaagatga cttctcaatc
    22261 aacgattgat tataaatcgg ttgggtttcc aaaatactta gatttgaaat tacatgaaag
    22321 tcttttaaat caacgagtcg tagtagaatt ttatcataag ggaaagaaat ttcatgaaga
    22381 acgtgtcaaa gtaggctata aaggatttag tgaaatcaat tttgattgtc atttagttgc
    22441 aggtgatcaa gtttgtttaa aatacgatca tcaaggaaaa gaatatgtgg cttggtatga
    22501 attttctcaa agagtattga aagacgaaga gggtaatatc gtttatcgga aaaaacgagg
    22561 agaaacccat ttgccagaat tagaaaaaga agccgttcta acattagatg aggaaaaaag
    22621 taacttatta gtagagtcta ttcggatgca ctataccgat aaaccaatgg ctatttttat
    22681 gcttacacct gattatgatc catctaacca tatcattgtt agtatttttc ttagtcagtt
    22741 atataaagaa ttatctacgc aatgtgttaa gactaaagga gataaatgtc accgcaggat
    22801 tcattttatt ttagatgaat ttgggaatag tgtgacctgt ttagcctaag gataaaagag
    22861 gctagttcca acgaacattg gaataacttt actatcgaga aatcaaatga cggggtaacg
    22921 ccctgaaggg tttccactga tactgcgaca tgcgttagta ggatgtgcat ccaaacgcgt
    22981 atgaagctcg ctgaagtcgg cagaagggta ccgtaaggaa agacacgata cgtatctacc
    23041 acgcaagtgc tctagaggta gggtatgtac ctgatatgtc gggtgtccta aaaagttcaa
    23101 taaggtgaga atgatttgat tatctgacaa acttgcgaat ggaatgtctg aaacgaataa
    23161 ctcatagaaa tgtgaggggg aagggggata cgtcctcttt ccattgactc gaggttgagt
    23221 aaaaatgtgt ttctatgaaa aaccttgcat ggttaaaggc tatggagtag tcaacaggca
    23281 aaagcaagca cctaagttga aatctatgga tagatagtaa ggaacagggg aagcatctag
    23341 cacggaggga gcataccgaa gatgtgtttc taaggaaaaa ccgaaatcgg ttataactta
    23401 ggtgaataga tgtgaaagta gagccacgac cagtgaaaat ttcgtaatga aattggagga
    23461 acaggctcaa gtcgttattt ataatataag aaacctattt aaaatcatgg attctcgtaa
    23521 gactaaaaag gtcacgaacc agaatggagc gtgataaaac catgactaac acaaaaggaa
    23581 ttagcttaaa gaacaaacaa ctcagacacg ccgaatacta cgacatgact gagactttcg
    23641 atgaattgca cgcaatgagt gttgcaaaat caaattttaa aaatctcatg aaattcatta
    23701 ttgatgacaa taatatcttt ctagcgtata gaaatatcaa aaagaataaa ggaagtgcaa
    23761 cggccggata cgatggtgtc actataaaag acattgaaaa attggcatgt ggtgactttt
    23821 taactattat taaaaggcga tttaaggatt acagaccaag aaaagttaaa agagtagaga
    23881 tcccgaaacc aaacggaaaa cttcgacctt taggaattcc ttcaatatgg gacaggatag
    23941 tccagcaatg tattctacaa attttagaac caatatgcga agcaaaattt aataagcata
    24001 gctatggatt cagaccaaat tgttctacag aaaacgccat agcagattgt cttgctagaa
    24061 tcaatcaaag taagatgaat tatgttgtag atattgatat aaaaggattc tttgacgaag
    24121 taaaccatac caaacttatg cgtcaaatat ggacaatggg gattcgagat aaacaactcc
    24181 ttgtaattat tcgtaagatg ttaaaggctc cgattgttat gcgaaatggc aatgttgttt
    24241 ttccaaccaa gggtactcca caaggaggga ttttaagtcc actgcttgcg aatatatgcc
    24301 tgaatgaatt tgattggtgg atagctaatc aatgggagga acgaactgtc aaagagctga
    24361 aacaccacta tcgaaaaaac ggagcatggg ataaaggaaa tatctattcg aaattaagaa
    24421 gccacacaag tttaaaagag atgtatctgg tacgctatgc agatgatttt aaaattttct
    24481 gccgaacagg agaagaagcc caaagggctt tttatgccac acaaaaatgg ctcagagaaa
    24541 gattaaagct cccaatttca atggagaaat ctaaagttac gaacctaaat aaagctgaaa
    24601 gtgaattctt gggtttttca ataagactcc acaaaaaggg aaaaaagaaa gtggctttca
    24661 cgtacgtttg tcgcaaagca gaagaaaaca ttaaaaataa actaaaacaa cagattaaaa
    24721 aaattcaata cgctggagca aaaaaaaata ggcaccgagc aataggtgac tacaacatga
    24781 tggtcatcgg tatccacaat tactatcgga tagcttcaca ttgtagtgtc aatttcactg
    24841 agatttatag aagtgttagg acagtaatag aagcaagact gaaagctaga ggaatgacga
    24901 aaaatggaat ctatggacaa gatcatgaag gataccgtcc gtatctaaag tctaagcaaa
    24961 tgagatatat tgaaggtttt ccgatacttc caattggcta tattaaaaaa agaactccac
    25021 tcaacaagcg gagaaatcta aataaatata ccaaggaagg gagaaagctc attcatcaag
    25081 agcaacaagc tatagaaagc tggaaaatca agtatcttgt aaaaggtttt agtaacaata
    25141 agaaatacac ggtggaattt tctgataaca aaatatctaa gtttatttcc caaaaaggga
    25201 aatgcgctat cacaggaaac gaacttttga tggaagaaat tgaatgccat cacattaacc
    25261 catatcattt gtccaaagac gatagttaca agaaccttgt cattatctct agagaaatcc
    25321 atagattaat ccatgcaact agtgaagaaa ctattctgaa actgcttcgg ttactgaaac
    25381 taaatcacta tcaaattaga aaactaaaca cgtatcgaaa aaaagcagct aattttccaa
    25441 tatcaatcaa aatgaattag ataggaaaca aattatattg taattaacga tggaacgccg
    25501 tgtgcggtga aagtcgcacg cacggtgtgg accgggggaa aagacggaga taatatcaaa
    25561 ctcttaccta tcggaattgc caccaatgga taatatggaa ggaatcatga cggtcacagc
    25621 tggccgaaac atgttgtggg atctgtttat tcaatcttat cagcaacttt atgcgaagta
    25681 tggagaacaa aacgcagcga ttatcaaaga aaattgtcag acacatattt atattatgtc
    25741 gacagatgat aataccatcg aagaattttc taaaaaagtg gggaataaga cggttgaaca
    25801 agagaacagt accatcaatt cattaggtat gaatactcat atcaatcgca atgttgattc
    25861 agatcgtatc ttaactcctg aaagaatttc tactttttta gaaggtgaaa cgattgtact
    25921 cgctccttta aaacgacaag atttgaaagg tcataaaata cggccgtatc ctatttttaa
    25981 tcatggggaa acgaatcttc cttatgctta tcaatatatt gaagatttca atccatcgtg
    26041 ggatatcaat gatatagaca tacattgtac acatgcggac ttggatctta aggctttaca
    26101 aattgattat gattatttcc taccagatga atatgctaga aatctctatc atggactgca
    26161 tgatcaatta gaagaaaaaa acgaagaaaa tatgttttat aaaatcgttc aatctcttga
    26221 gaatgagacg gctaaaaaat tgttaattca attttacgaa gaacaagcag cagatcgact
    26281 tcaagaatat gttgaaaagt tgatcaaaaa tgaagaaatt tctgaaatga ttgggaaacg
    26341 gttgttattg gaatctgaaa aggtggtgaa agaatgatca ttggaggaat tttttctggc
    26401 atagccgact ggtggtcagg cttattcgga gaaggggatg caaaccccaa aaccgtaacg
    26461 aaagtcttac aagattatgg taattatctg cagtatcaag atgtgttcag ttggatttgt
    26521 aatacttttt tatgggggtt gatcaaaggg ttatataatc tcaatacttt actagaaaaa
    26581 accatttatc aaagctttga tttaaaagac ttattgaacg ccgctggggt aaatgaacta
    26641 tttcaaagtt tgattagtaa ggtattggct cttttttgtg tcgtcacatt aatttattta
    26701 ggcttgaaat tttttatgag taaacaccca ccaaaagtca aaaatatctt ggttaattta
    26761 tttatggcca tgatcttgat ttttggtggt agttctttaa tcgatgaagg gttaaatatc
    26821 agtaaaagct tttacggaga cgtcactgca gcaaataaag cagataaaac gggttcacct
    26881 gcttttcaaa ttattcaaaa taatgtgatc gatgtcagta cattactagc aactgacccg
    26941 aataaagtag aatccctgcc aacagacaaa agaaatgctt taacagcgaa aaattttaaa
    27001 acagcgaata tcaatgggat tattactcct aaacaagcta aagaaatggc ggataaagat
    27061 aagagtggtt tatcagattt acaaaaggat cggttaagag cgttacaata ccgactggaa
    27121 ctggatgata atggaaaaga aattcctgtg caaatcagtg atgaaggctt agccaaatat
    27181 gtttatactt cagggtatcg tcgatacatt agtagtccag ggattatttt agctggtgaa
    27241 tcttctttgg cggtggccta tttatttatc ctatttacga tcgttacttg cattattgag
    27301 ctggtattta aaaaattcta tttagtgatt gccgcgtcaa cggactttga gacaggacaa
    27361 cggatgaaaa gtgctatcga agatatttct caatccttct tgttattggc atttacaatt
    27421 ttagaattac gaatctatac cttaatgctt tcaggtattg gtgatttgca tgcggcagga
    27481 aagatcaatg gattcttgta tgttgtcgct ttgattgtac taaccattgc gctattcaaa
    27541 ggctctcaat cagttacgaa aattttcggc gttgatacga gtctgaaaaa cggcagtaat
    27601 tctcttctta gcatgtttgc cttaggaaat attgcgaaaa atgtgggtag cggagctaaa
    27661 aaccttgcaa gtgctggtaa gagtggttta agccatttaa atgatattcg taaaaatgga
    27721 tttggccatc gaaaagatgt tacccctgat aacggggaag aacaatcaga cgataaagct
    27781 acaactgtac ctaatagtaa gaaaacagtt tccgaaatgt tcgggaaagc gaaaaatggt
    27841 gtttctaaag ccgcagaagg tcttggttac gtcaatgaac gtgggttaaa aggaacagct
    27901 gaagatctta cagaaaaagc caaagacacc ttagaagata aattagacgg cacgagagct
    27961 aaagaattgg ctgaagctgt gaagaatcca tccgaagcac tagaaaaaca aaaagaaaaa
    28021 gcttcagaag tcaaagaagg actaaaagat gcgtatggtg aaggacaagt caatgcagca
    28081 gtgaaagcta atacaacaac cgaaaaagaa aatcaagaag gtatagtgat tccttcagtc
    28141 aaagatcacc aagaagcgtt gcactctaat gaaaattcga ttgaagagaa aaattccata
    28201 gaagaaagaa attcagaaga aggaaaaaat ggacagttac caaaaactga tccagaacaa
    28261 tatcgctata aacctaacag tgaccttgaa aatgagaagc gaatttcaga tttatcaaaa
    28321 gtaaatcaag aagacaaagc agaaaatgag ggacagttac caaaaactga tccagaacaa
    28381 tatcggtata gatctgttaa ctccgaaaat gaaaaacgca tttcagattt accaaaacga
    28441 aatcaagaag tacaaacgga tgaggattcc atcgttctgc caaatgataa tggaatcgaa
    28501 gttggaactg aaaaatcaac gaaaattggt ttacctaagg cacaaaattt accaatgaaa
    28561 gatagtcgtt tgaatcaatt caaggataat gatcaaagtt ttgttcatca atcacctgca
    28621 ggtactgttc aaatgaaacc aagtcgtttg cctgaaaatg gaaatctgac aactgtttct
    28681 tacaatttat ctgaaagtgg tgagacaggg aatgcgtccg ttcaaaatgt tgtgacaaga
    28741 gaagctgcac caaaacctat tcaaaacgtt catacaggtt acaagccagc aaatgtggca
    28801 tttactgcac caacaaaaat aaatgtgaca agagaaacta aaaacaggaa caatgcattg
    28861 aaagaacaac tattgaggga tatcaaacag gatatcaaac cataaaaggc ctacaagtta
    28921 ggccttttct ttatatgaaa aggagaaatc aattatggat aaatatataa acatgaaaaa
    28981 ccctccaaga tttagtggag aatggaagtt tttcgctttt ttctttttag atctattaac
    29041 gcttggtggc tgcttttttt gggcgaaaat ggtcaataat cttgtccact taccactctt
    29101 ttttgcactc ttgaattatg gcttatggat tgcgatagcg ttagtgattg tttggcgacc
    29161 ctataataac ccaggtcaac gacaaatttt tgcgattatc gagtctattt tttttgcgga
    29221 caagaaccat tatcatgcgt tagatcctaa ccgaaattat catggtacgt ataaggagga
    29281 tgaataaatg cgagtcaaag gacaaaattc agttgaaagg aaatccattt tgcagtttgg
    29341 aaaaccagaa gagcgctcta tcttggactt atttccgtat aaatcctgta cagaagaggg
    29401 agtcttaatt actagagaag atcattttca acgttattat cgtgtagttt ctacagacgt
    29461 ggaaggatta aatgaacaag agaaactaga acgaatgaat cagttgacca ccgtcatgcg
    29521 tacgtatgtg ccaacaatta agttagttag tttgaccaca gagacggatc taacggaaca
    29581 aatcgtgcaa aagcggcagt tattagataa aaatcgtatg gaacagtcct taggaagaaa
    29641 tctacagcga ttaagaaaat atgaaaagaa attagtagag gaaatccagg aattgaaacg
    29701 agcagaacag gaacgccctg atttaagctt ctttttcgtc attgaaggaa agacaatcaa
    29761 ggatttgcaa accagaaacc gtcagttaat gcgatctagt ggcattttag ggttaaaacc
    29821 actctacaaa aaagaattga tcaagattct ttatcgaatg aataatatga atgatgagta
    29881 ggaatgggga agagagaatg aaatatccgt tgatatggtt agaaaaagat gttccgaaag
    29941 aagagaccac tagaaaattt cctcacactt taagcctggg taagttggga aaaggaaaat
    30001 cttttaacca agtaaagaat caaacaagaa gaggtgactg tacatgttag aagcaattaa
    30061 aacagctttt tcaccacaaa atgatcaaaa ggtacttgat ccattaaagc aactaggaaa
    30121 tcgggaaata gaaattttaa aatccaaagg gtatgattta gactttttaa aacaagtgat
    30181 gccacaaggt ggattaaatt tcgatgaaga gtatgtgacc tatggaaatg gttattcacg
    30241 atgtgtgaca gtttatcact acacaaaaaa tcctacgctt ttttggttgg ccaatctcat
    30301 gaacaatcct catacagtgg caacactaga tgttagcaca gatgaaaagc aaaaagtgat
    30361 tgatcaaatc aatcgtgcct tagatgaatt aaatgatcgg tcagataatg aacgaaaagc
    30421 gactgaccga aataaaagcg cggaagaata cctagaacta gcggaatatg cccgaaaatt
    30481 gacttctaat ggggaaattt ctaaacaagt caaagtgaga gtacatgttt acggaaacac
    30541 gattcaagaa gtagatgaac gagcacagga actgatcgaa aatctcaaag ggatggatta
    30601 tcgtgcagtg acctatctgc acaaaacgaa aagagactgg caatcgatgt tcaccatgtt
    30661 cgatgaacaa gctgaatggt ttggaaatat caaagggcaa tcactaccat cgctaaacat
    30721 tggcggaggt ttcccgttcc accaccaaac gctaaaagat ccaggtggaa cacaatatgg
    30781 ggaaaccatg acaggcggcc cgtttgtttg gaatgccacg tataaaaata gtgtgcggtt
    30841 gtctaccaat accttgcttc taggaatgat gggagctggt aaatcgaccg ttcttaaaat
    30901 gatcgcggaa gcccatttag ctgcaggaga ctttatttgg ggctttgaaa agggaaaaga
    30961 ctttattccc tttttaaagg aatataacgg cattatggtg cgtttagacg gatctgacgg
    31021 gatgatcaat cctttagaaa tatttgccac acgtacgtat gatgaagctt ccagtttata
    31081 tgacgatgga tcagtcaaag atttgaagat caatgaagca gcgtcctatc aaacgcactt
    31141 agacaaagtg gtctatcagg tacagttagt gagtcctcag ctaaaaggaa cgatgaaagc
    31201 agaatttaag acctatttaa atcgttttta tgaagagtat ggaacagtac cacgcggctt
    31261 tacttcctca aattcgcgtt caaatactga tacgcaagtc acaggtaaag atccagaagc
    31321 ttatccgacg ttcaaagaat ttttagactt tttaggacaa ctggaattac caggtgcttc
    31381 ccaagagaaa aagaatcgaa aagaagaaat ggaatccatc gtagaatcct tatgtgagac
    31441 gtatggcatg atttttgatg gtcatagtac gattcgacac cttgaccaac aacagttggt
    31501 cttttttgac attgactcta ttacaggctt agatcgaggg gtacaacaat gtcaaatgta
    31561 catggctatg acgttgattt ggaatcaggc attgattcaa ggaagaagac aacgacgttt
    31621 aattaaagaa aaacaaatgg aagaacaaga cttcaaatgc ttcttggcga tgattgatga
    31681 gtgccataat ggtgcgacct gttctgcagt gaaaagctgt gggttgcaag tagtctaaaa
    31741 actattgtaa taactcttat aacgagaagt caaatgagtg ggtaacgcca caaagggctt
    31801 ccactgatac tccgacatgc aacaagtagt ttatgcagac caacgttgtg tgaagctcgg
    31861 tgaagtcggc tgaaggatac cgtaaggtaa aagaaatagt gaataccacg aaagttctct
    31921 agaggtaaag ggtgtatccg ataagtcggg tgtccaaaaa aacttaatat ggtgagtatg
    31981 tacaaacgta ctgacgaact agcgaatgta ctgtccgaaa ggcatagctc ctagaaatgg
    32041 gagagacagt gagcaaaaaa tcactcattg tgctaacttg tggttgagta aaagtcgttg
    32101 ttatgaaaga ccatgctgcg ttacaggcgt aggagaagtt agggggcaaa agctagcatc
    32161 taagttaagg catgtggata gatataaggg aacaggggaa gccatcaaca tggagagtgc
    32221 atactcgatg aagtggtagt aagaaatatg tgaatgacaa ataacttact ttagtgatgg
    32281 tgaaagtaga gtcacgactg atgaaggttc tgtaatggaa ctggaggaac aggctcgatt
    32341 cgtctgtaaa agtcatgaaa cccgttcaac catgggattc tcgtatgact aaaaaggtca
    32401 cgagccaatt ggagcgtgat aaaccatgac aaaagaaaca agtccaaaag ataggaagtt
    32461 gcggcatgca gaatattatg gcatgacgga aatcttcgat aagatatact ccaaaagttt
    32521 gaagggagat aatttcaaga atctaatgca gattatcatt tctgataaca acattgtcct
    32581 agcatatcgt aatatcaaga gaaatcacgg tagtgtgaca agaggagaag atggagtaac
    32641 gattaaagat attgaaaatc tcaaccgtaa tgaattcgta gaaattgtta ggaagcgatt
    32701 cgcacattat aaccctcgga aagtaaggcg tgtagaaatt cctaaatata atggtaaatt
    32761 aagaccgttg ggtattccat caatatggga tagaatcgcg cagcaatgca tcttacaagt
    32821 attagaacca atatgtgaag caaaattcaa taatcacagc tatggtttcc gcccaaatag
    32881 atcagcagaa cacgcaatag cagattgtat ggcccgaatg aatcaaagtc acatggaata
    32941 tgtggtagat gtagatatca aaggtttctt cgacgaagtc aatcatacta aactcatgag
    33001 acaaatctgg tctatgggta tacgagacaa acagcttctc ttaatcatta ggaaaatgtt
    33061 aagagcaccc gtagtacttc caaatgggaa gttgatatac ccaacaaaag gaacaccaca
    33121 aggcggaata cttagcccat tacttgcgaa catcaatctc aacgaatttg actggtggat
    33181 agcaaatcag tgggaggaaa aagatgccaa agagctaaaa catcatttta ggaaaaatgg
    33241 agcttgggat aaaggtaata tctatcacaa gctcagaaag agtacaactc taaaagaaat
    33301 atacctagtg agatacgcag atgattttaa aattttctgt agaaattata atgaagcgaa
    33361 aaaagttttc tatgcaacta aaatgtggtt acaggagcgt ctaaagttac caatatctga
    33421 agaaaagtca aaaatcacaa atctttcaaa agaagaaagt gaatttttag gctttacgat
    33481 gaaattagtg accaaaggta acaaaaaggt tgttaatacg catataggta caaaagcaca
    33541 aagcatgatt aaagtgaaac taaaaaaaca aatcaaacga attcaaaaaa gtcctaatag
    33601 caaagatacc tttatctata ttggagtcta taacagtatg gttattggga ttcataatta
    33661 cttcaaaatt gcgactcatt gtagtgtagc aatgcgaaaa attgcaagag aactcagacc
    33721 aagttttgaa atacgactac gagaacaagg aatctcaaaa gaaggacaat acggaaagta
    33781 cgaaggatac aagccttacc tacggtcagg acaaatacga tatataagag ggtttccaat
    33841 attaccagta gggtatattc aacacagaca tccgatgtgt aaaaaaagaa atattaataa
    33901 atacaccgaa gaaggtaggg aagccattca taagaagcaa caaaccgtag aagaatggaa
    33961 gatatcgtgg ttgtgtagca atccagtgct tagtaagaga gcaacgattg aatttgcgga
    34021 caatcgaatt tcaaaattca tcgctcaaaa gggaagatgt gcagtaacag gtgaggagct
    34081 tatcttatct gaaatgcatt gtcatcatat tatcccttat catgaaagca aaagtgatag
    34141 ctatgaaaac ctagtgattg ttactgagga ggttcatcgg gtaatacacg ctacacaaag
    34201 tgaaacgatt gaggaacttc taaaatatct aaaattaaat ccaaaacaaa aagaaaaact
    34261 caatgagttg agattaaaag ttggtaacga agaaatatct taataaatat ttgtaggacg
    34321 ggaaaacaga atgtctttta tagacgaggg aacgccgtat gcggtgaaag tcgcacgtac
    34381 ggtgtggact gggggaaaag acggagataa tatcaaactc ttacctatca gtatcgatta
    34441 atccagattt tattgaaacg gtaaattatg tcacaaactt tcaacgagaa atgcgtaaag
    34501 tagaagcaat gactgtttta gctacacaat ccccacaaga aatggtgcca gaaaatatgt
    34561 cttctgataa cttctcgaaa ctgaagaaag tctttgaatt ttcttctacg aagatcttta
    34621 tgcgtatgga tgaatctatt ttacgtcata tagaaggatt agttggtaaa tcaatgacaa
    34681 actcagaatt agaatccatt cctcaacaaa atcaaggaga cgctgtgatc aattttggct
    34741 caaaagaaac cattcgtgtt cattttagtc cgaacaaacg tcaattaaaa ttgtttgatg
    34801 gaggaaaata atggaaaacc aaaatgaaag tttaatcaag cagtatgtta agcgtcgtgc
    34861 aaagcgacgc ttatttttgt ggttgtttgg tacttctgca ggtttgatta ctattttgat
    34921 tactgtgttc gtgacccttt ttctaatact tgcggcgggt tcgattgata attctgattc
    34981 agattcaagt agtggtggcg aagcttttac aggagaatat tcggaaggct taccgattta
    35041 taaagaaatc aaaggaagag gccctttttc tgatgagatt gcgcaatatg ctgtaggtgc
    35101 agcagtgaaa tataaattat taccaagtgt gatcctttcg caatacggtt atgaatcagc
    35161 gtttggtacg tcggcgtccg caagaaatga tctcaactat tttgggatta cttggtttga
    35221 tggttgtctg tttcctaaag gaactgcgcg aggaatcggt ggtattgaag gtggctggta
    35281 catgaagttt cccaactcaa aagcagcctt ctcctattac ggctttatgg tagccacaca
    35341 atccaatttt aatgcatgtg taggaaataa aagccctggc gcaagcttat tgatcttagg
    35401 acgaggtggt tatgccgctg caggaattac tgaagatagt ccgtattata caggttgtat
    35461 gtccattatc acttctaata aattaaccga atatgatgaa ttcgccatca aacattgggg
    35521 tgaaggtggc aataacaacg gcacgatcac tggtgaatgg acgaatccat tccctggcag
    35581 cagtttagat aaaagttcct tcagtggtgg tcaattattt ggaacaaatc caggcggtga
    35641 attccgtccg aatggcttcc atgatggatt agactttggt tcagtcgatc atccaggaag
    35701 tgaaattcac gcggtgcatg gaggaaaagt cgtgtacgtt ggcaatcctg gtatttcagg
    35761 tttaggtgca tgtgtgatcg tgattaatta cgacggtttg aatatggtat atcaagaatt
    35821 tgcgaatagt acaggaaatt ctcgtgtgaa ggttggcgat caagtgaaag ttggtcaagt
    35881 aatcgggata cgagatacag cacacttgca tttaggtttc actcgaatgg attggcgtca
    35941 agcacaagga cacgcattca cagacgatgg tacatggatt gatccattac catctttgaa
    36001 tagcagcaaa aaatagatca ggagaaaaaa tatggaaaat aaaactttaa aaatcgtctt
    36061 aacagctgca attagcgtta gtttaatcgg caatatcgcg ttgttttggc agctgaaaga
    36121 agaacaagag acaaatacct ttaaaattga agaagtggct aaaaacgcgt ctgagaagct
    36181 caaaaaaaat tcctctgagg cacaggcgaa gatcgacaag ttaaatcaaa ccatcaatga
    36241 gaaaaaccaa gaaatcgatc agctgaagca acaagatggc acaaaagcaa aagaggaagt
    36301 aactgaagca caaagaaaga cggcagaaga gttcggtcaa ttggccattg ataaatcatt
    36361 aagtagaaat gaattaacgg aaaaactaaa agatgtcgca acccaagaag tgatcgacaa
    36421 attaagtcct gcagacgatg atcaccaaca tgatgattac ggaagttact cctttacatt
    36481 aggtgacgtg caaacttatg ctcaaacaag cagtgtaaaa gaggaacaaa attttgtcgt
    36541 atttgttgat tatacaatcg gcaatccgca attcaaagat gtcaaaccac aaaaaataaa
    36601 aggtggatta actgtcactg aaaaacaagt agatggtcaa tggaaagtca tagactttag
    36661 ttatttcact cgttaggagg aagaaaatga gtaagaaaaa aggagaaaga caacgtttca
    36721 aaaaacgaaa aagttatctt caatggctgg gaatctttat gattctcggc tttttgattc
    36781 ttgctagtcc gatttatacg aatcagaagg tagatatacc agatgaggta agcacgaacg
    36841 cctttttaaa cacgaaagag ctgacaatgg tttcaaaaga aaaaggagcg aaccagcaat
    36901 tagtttttga atttgcgatc gatgatgaag atcaaagctt gttaaaagaa ctggctaatc
    36961 ttaattatcg cgtagaagta aagacagcta aaggggatta tcaagcaata cgagccaaag
    37021 tgatcaaggt gtctgatgat tattttgcag tgaagctaac gcatgtacca gaagaatata
    37081 tcgccatgcg tttaaccatt attcctgaaa aaattgatcc aaaagtggat atgcaagaac
    37141 cacaagatct gatttattac gtgcacgaag ataaggttaa agatcaagtg aaagatgaag
    37201 attatgaaca acacgcgatc aattacaaag tgaaaggata caagaaagaa caaaaagcag
    37261 ctcaaaaaca aatggaatct ttccaagcaa ccattgattt gaatgaagaa ttagtgaaga
    37321 agctaaaaga tcaattgcct tatcaagtcg cagatgatca ggaaaatact gaaaacaaga
    37381 ttaatagcta ccagcaagaa attgaaacat cgaaaacgca actgaaagat caagaagaga
    37441 tcattcaaaa acttcaagaa aaaattgatc gtatgacaaa agaaaagatt tagaaggaca
    37501 ggtggagtga aaaaatgaag cgaacaaact tggctgacaa agatactttc gctgagcaga
    37561 aagtattgat taaagcctat ctcaaagttg taaaagctaa agaaatatgt cgagaagtga
    37621 atttatctaa cacaacaata tctgagttga agaatggtcg aaagaattta gatactttaa
    37681 aattcagatc atttgaagct ctatatgtag ctgctaaaaa atattttaca gctgataaca
    37741 aataaaaaac aggagtattt tatgtgaaaa aagtatataa aaaatgctta tgaatcagct
    37801 atatcacctt aaccatttgt taatgagatg ggagaaaaca ttaaccacga ttctaacgca
    37861 aacatcaagc acataaaaca tttaacactc ctcctttctg gctgaagatt tcaaccaaac
    37921 aggaagacag cgtatcaatt atagcataca ctttttctta aattcatttt tcatatgaca
    37981 aaatatttcc ttctaatcga caaagttaga aggatttttt attgtctaaa tacacaaata
    38041 accttaattt ttaaagagca taaggaaacg tgatatcact ttctcacgaa aggtgttagg
    38101 taggctgtgc ctaccacgtt tctaagaaag ataaatgata tcaaaatttt ggatttgtgt
    38161 gtagatttgt gggtaggatg aaagcgaatg tgggcagtaa aataagcgtt tgtgtgtagc
    38221 aaaatcaaaa aatgtgtagg gatatgtgta gctacacata tccctacaca taataaattt
    38281 ggaggaaaat atggacttat taattagaaa tattgaagaa aagtatatta ataaaattga
    38341 caaacgttgt gaagaactta cggtcaagac tggcaaaaaa tggagtcgaa atcaatacct
    38401 aaaagttttg atcgaaaatg attttgatca cgctcttctg aattataaaa aagaccaatt
    38461 tgatcggttg ttagaaaaat ttgtggatat tcaaacttat aatacgaaaa ctttagaaga
    38521 atatattgca acaaataatc aattaattgg tttgttaatt gaataataaa aatagtattg
    38581 tgaatttata aaaggaggaa atcaattttg gcaaaagcaa caagaaaaca tgtatatctt
    38641 tacgatatgc atatcgaagt acttgatgaa ataatttcta ctcatgaaga tatatctaat
    38701 tatagtgaag cagtcagaca tttgtgtatg atgtatgatc caaaaatcga agaaaaaata
    38761 aatccaattc aaattaaatt gaatgcaatg ggaaaagaaa tttctatttt aaccgaaatg
    38821 gtttcagagt ttgcggatct tcatttgaaa gatacaggaa tactaattgg tacaaattca
    38881 actgtttatc aagaagcaga agaacgtgtg gaaggaaaaa ttaaatccaa tcaaaccaaa
    38941 aaatattctt ctaagttaaa gaaaccagtg aatgaatctg ttcataccag agtagatcca
    39001 tttgatccaa cgaagattct aaaaagataa aagtagaagg agaacgtgat gaataaatcg
    39061 cctgcagtta ttcttacttg tcaatttacc ttgcccaata aaaaatcttt ttcaacatac
    39121 attgattata tgacaagaga aaaagcactg gaagaaatgg aacgacgtac gcccgaagaa
    39181 gaacaggaac tgcaacgaat caagaatgct ttagaggatt tttatattcc tgaaggagaa
    39241 actttttcgg aaagtaagac ggataaagaa ttcagtgaaa cgtcgaatga agctcaatcg
    39301 ttgatggaac atggaatcaa ttttgacaag ttagaggaac aagattttac caaatatctt
    39361 tcttatatga cccgtcacta tgcgttagaa caaaagaaag aactgacgca aagggaacaa
    39421 aaagaatacc aacggttgaa ccaagcaatg aaaaaatatc aaacagagcc acataaaaaa
    39481 gcttcaggaa atcttcctgg agttttttct atggcttcag acgaagtgaa aggaagtgat
    39541 ctgaaggaga tcaaacaaat cttccaaaga ggacaacaaa aaggctcaat catttatcaa
    39601 gatgttatct ctcacgacaa ttcgtatctt gaaaaaatgg gcttgtacga tcccaaaaca
    39661 gataccttag atgaagaagg tttgaaagca gctggaagaa aaatgatgga gacattattc
    39721 gagaaagaac aattgaatga aacaggttac tggatggcaa cgattcaccg taacactaag
    39781 catatccata ttcattttgc agttgttgaa aaagaaaata cgcgtaaacc gtatactgag
    39841 attgaaaatg gtgtgagtta tatcgtaccg aaggggaaga gaagacaagc aacattagac
    39901 gcaatgaaaa ccaattatgt acatgagtta gaacgtttct cttatgaaaa ggatcgaatg
    39961 atcggtatgg agaaacgaaa tttattgaca cgaaaaagtg atttgcgtaa caccttaaca
    40021 aaagtagtaa aagaaccagc acgttacgat cgacacgcca tgaacttatt aaatgaagta
    40081 tataaaaccc tacctgaaaa acgttcggaa tggaattatg gagatgaaaa tcgaaccaag
    40141 ctttcaccat ttacaagggg aaaacttgat gagttaacca aatatgtttt aacaaaagaa
    40201 cctggatatc aagaatatat acagatttca aaagcattga aagaagaagc caaaagcctt
    40261 tatggggaaa gcaaaagaga aacaaagaat gcagaaaaaa acgccctctt tgatttaaaa
    40321 aagcgcacag gaaatgcgat tttaacagaa ctgaagagac aggatagtca aataaaacca
    40381 ttaatagaaa gtgtcaatca gatgcaatct atttacaatg aacgatcctt tcgaacactc
    40441 aaacagttgc caacaaatga atacaagtat agttatcaag aattcgtaaa gcgtaggaaa
    40501 caattccaac aaagagttgt cacgcagcga agcttgcgtc aattaaatcg aacaattaat
    40561 gcacatgaag aaaaatacca cgcaagtaaa gcatatgaag agcaacagcg aagaatccaa
    40621 atggaacaag aaagacaata ggaggaagag aaatgaaaca atatttagat caatggaaag
    40681 tcatagaagg atcactaaaa gaagaacgaa tcgaacaact tcctgattgt ttagaaaaag
    40741 agcatttgtt tcagataagc gaaatgttga gaaacgaaca gtttgatcca aatcaatttt
    40801 tagttgttga gtacccagct actggtgtgt attgttgtaa tcatgtgaag ggtgaaaaat
    40861 attttattat tcaagaatac gaaggtaagt tagcgcctta ttatactact tgggaaataa
    40921 atgaagaggg aatcaataat ttcccatgtg aaagcattga agagtctatt tctctcacgg
    40981 aatgttaaat aaatagagac gtaaaagagt cctgaacgtg attgtccagg actctttttc
    41041 aatgaattgt tttccgccaa ttcactgagt ggtgcgattc aattttatta tatcaaaaga
    41101 aagtgagatt ccaaagtgaa gggaggaaga agaaatgact cagacaacga ttggtgttgt
    41161 ggttgaaaaa agcagggata atctcgtttt tgtgacagaa attcaaacag gtagagcttt
    41221 tgtagtcaca gacaaagctg caaaagcgta tcaaaatggc aatattttaa cgttaaatct
    41281 gactacgaag atgttcgttg atgcagtaga aaattatcca tttgtttaga caaacgatat
    41341 tttacaagga ggaaacggac atggcagaag aaaaagaacc actagataat agtcgtttgg
    41401 gaaagtccaa aagaaaatta gtacggttgc aaaacgaatt aaataatcaa tttcaaaagc
    41461 tatttgatca ccaaaaattg acaaatgggc aaccaatgaa tgacaaacga aatggtcgtt
    41521 catggtttaa ccagcaagaa aaaatagaaa ataaaataca aagtttgaga gaagagatca
    41581 agcaacaaga aaaacaggta gaaaaacttg aaagacaaga agagctaaag gaaatgggtt
    41641 ataacaagta cggtggcctg gacatgacga tcgaaaatat tccaagaatc aaagaagaaa
    41701 tcgaacgctt tgaaaaaggt gaatcgactt tttcagctgc cacgatccga aaataccaac
    41761 gaaaattgga aacattagaa cagctgaaag aaaaatcaga gaaaggaaaa gaaaatcttc
    41821 ttcctgaagt acaagcgatc attgacagcg gacgagtgac gcaatggaaa aagaatccaa
    41881 ctatctattt tctgaaaggc tatcgaaaag tcgctttaga actcaatgaa aacgggggat
    41941 ttgaggaatc taaaaaatac caagccacgt cggaagaaga aaaggcagct gttcgtgaat
    42001 tacttgaaga agctacacct actgattctg tgaaggagaa agaaaccgat catttctatc
    42061 gagtagaatt caacgaaaat gatccagcaa aagggttgaa aagttatggt ggggagatcg
    42121 taacactaga attgatcgat caactcaaaa cactggataa ccaatacgcc gcagaagcag
    42181 gctattataa attctatttt gaagaagtga agaatggaga agtagtcgat cagatacgaa
    42241 tggatctagg ggatggcgtc aatagtaatc accccatcta tttagagtta gctagattgt
    42301 gcgaacaatc acctgaaaag gaacaagaac aaacagtcaa aaatgaacct atttcaaaag
    42361 tacaaaacga gattaaagag acaaaaacaa aacaaaaggg ttatcaaaaa cgaacggcag
    42421 aagagattaa gcaagaagtc aaatcacttt cccaacaggc gcttgatgcg gtgaaaaagc
    42481 atactcattc acctgaagac gtcaaagaac ttttgaattt tatgtctcgt tttccagaac
    42541 gatcctttag aaatcaatta ttgatcgagg aacaatttcc aggcgcaacg gcttgtttag
    42601 gtcgtgccgc gttgaaaaaa gaaggaatct atatcaaaaa aggagaacaa gggaacaaga
    42661 tctttgtcag aaaaacggtc aaaggatttt atgagaaagg acgtggcttt gttcgagaaa
    42721 cagaggctac accagaagat aaaaaacgaa tcgaaagtgg tcaaatcgaa gtgatcaaaa
    42781 aaccttacta cacgattgaa aaagtgtttg atatcacaca aacacaattg aaacctgaag
    42841 actatccgaa aatcttcccg aaccgcgtct ttgattttca attggatcaa acagggcaga
    42901 cagaacttca agttgggatt caagcggtag ccaaaaatat tggaattcaa attcgagata
    42961 tgacagaaag tgaagtctat caaagagaat taggacaagc acgtggggcg tacgttcgta
    43021 acgaatttac tggcgaagaa gaaatcgtca tgaatactcg aaatactcct acgcagcatt
    43081 tagccacaag tattcacgaa ttagcccatg ctagaatgca caaattttca gaattagata
    43141 ctgcaacaaa agaactacag gcagaaatga cctcctatat tgtgtgcaag cattttggga
    43201 tggacacctc ggagaaagct attccttata ttgcgacatg gacgaagaat agtcagacgt
    43261 tggatgataa ggaagctgcg gaacgtggga aaatcatgaa tgatgtcagt cgtgtcgcaa
    43321 atgaattcat tcaaaccatt tctaatgaaa tcaatcaata cagagaaaga gaaccagaga
    43381 tccagccagc agaagtgaag caggagcata aaaatactgt agaaacggca gaacaaaaga
    43441 atattgtgcc cgttggctca ctttggatcg acaagaaaga cgggaaagtc ctggaagaag
    43501 acacgggccg aacgttacat ttgaaggatg cggcgtttat tgaagatccg cagcataatc
    43561 aagtgatttt aaaaggaaaa agttatggaa aagagatcca aacggcgtta ccttcagaag
    43621 aatttgcgct aggaaatcca ttccgatttt cacttgagac agtgtcagaa gtggcaaaac
    43681 gaaaaaatga aatctattat gaacaacaat tgtcacaaaa agaacgcgta taaaacaagc
    43741 aagaaagact atctgaaaaa tggatagtct tttttctttt gggaggtttg aaaatgacag
    43801 agaaaaaaat gagtctcatt gatcgatgca agcaaatgga tattgtggat tttgcacgaa
    43861 acaatggttt agcggtggcc aataaaggac gagactatcg cttagaagac catgattcat
    43921 ttgtttttga tcgaagaaaa caacgttttt attggaacag tcaaaatatt agtggcgaca
    43981 ttattgaatt agctaaactt tttttcatag acaaagagat ccaagaccct aagcaacaat
    44041 ttaaagcagc cactgatttt attctaaaga atgaagacaa aacagaacga gtggaaaatc
    44101 ttcattttga gacagaaaag tacaaagatc accccgttga ttatcaacca ctcacagaaa
    44161 aaggaaggaa ttatctgaag gaagaacgaa aactgcccga ttggttgatt gattatgcag
    44221 aaaaagaagg attgattgcc gaactaaagc caaaacatga acggcaaaat ttcttagttc
    44281 gagacgatcg tttggatcat gcggtcgcct ttttatggaa agatccacag acaaaagaga
    44341 cagtaggggc gagttatcaa ggaacgttca ttgattatga acgttttggt gagagaggca
    44401 catataagca tatagataaa aattcgacag cgaatcatgg ctttaaccta aagattggtg
    44461 atcctaaaca gttaaaattt tttgagagta gtattgattt acttagctac gctgcactga
    44521 atcgtgatca gttaaacgat acgtggttag tctctatgga agggttaaaa caccatgtga
    44581 tcagtcatta ttttggtgag gcagtatctg aattgagaaa gaaacaagcc tttcctcaat
    44641 ccatcgagat ttgtgtggat aatgaccgtg caggacatat tttctacgaa aaagaacaat
    44701 taatgggcgc agttgatcca tttacgaatc agaaagtacg ttgtgaacga ggaatcgcta
    44761 atgattggca agtgccaaga gaatacaaag tcatttatga agaagtcgca aaagaagaaa
    44821 aggtcacacc agaagctatt atggccatac acaagacaga aaacaatctg caattaacga
    44881 accaactggt ttccgctcac aaagtcaatg cgtcttttgg tcagcagctt tctgttaacg
    44941 attcaataga agtcattagt ttgaaagata tttgtagaga agtcgcaaaa gaattaaaag
    45001 attgtgaacg ctcgaatggt acgtatgatt ttgatcgttt ttatcaagga aaaggggata
    45061 ttaattctca gattcttttc tcttataaag cagaacaata ctacaaaggc tataaaaatc
    45121 atgaacatga atttgtacct gaagtgaaaa aggattggaa cgatcaattg aagcatgaga
    45181 tccatcaaca ggaaattaga aagcaaaaac gtgcgatgtt gtttcaacaa ggcagacaac
    45241 aagaaaggga gtgagatcgt cacgatgaaa actgtcattt tagcagaaaa accgtcacaa
    45301 gcgaaggcct atgccgatag cttttctaaa gccacccgaa aagacgggta ttttgaaatc
    45361 caagatcgat tgttttcggg tgaaactgtg atcacgtatg gttttggtca tttagtcgag
    45421 ttagattcgc ccgatatgta cgatgaaaat tggaaacaat ggtcattaga gcatttgcct
    45481 attttcccta cacattatca ctatcatgtt cctaaagata agaaaaagca attcaacgtg
    45541 gtgaaacagc aacttcaatc ggccgacacc attattattg cgacagatag cgatcgtgaa
    45601 ggggaactga tcgcttggac gatcattcaa caagcaggcg ctgatcaagg aaaaaccttt
    45661 aaaagacttt ggatcaactc cttagaaaaa gaagccattt atcagggatt ccagcaatta
    45721 cgagacgcag aagagaccta tcccaaattt gaagaagcac aagcacgcca gatcgctgat
    45781 tggttgatcg gcatgaacgg cagcccactg tatagtttac tcttgcagca aaagggcata
    45841 ccaggaagtt tttccttagg cagagtccaa accccaacgc tttacatgat ttatcagctg
    45901 caggaaaaga ttagaaactt ccaaaaagag ccttattttg aagggaaagc ccaagtaacc
    45961 gctcaaaatg gcatatttga tgcgaagctt gatcccaatg aaacccaagc gacgcaagaa
    46021 gcatttgaag cctatttaaa agaaaaaggt gttcaagtag gaaagcagcc aggcacgatt
    46081 cttgaagtcg aaaccgagaa aaaaagcgcg gcaagtccac gtcttttttc tctatccagc
    46141 cttcagtcta aaatgaatca gctcatgaaa gctagtgcca aagatacttt ggaagccatg
    46201 caagggttgt atgaagggaa atatctaagc tatccacgaa cagacacccc ttatattaca
    46261 gaaggagagc atgcgtatct gttggatcat ttagatgaat acaagcattt tttaaaggct
    46321 gaatcattcc ctactcctat tcatacaccg aatagtcggt atgtgaataa taagaaagtc
    46381 caggaacatt acgcgatcat tcctacaaaa acagttatga cagaagcaac gtatgaaaag
    46441 ctgacacctt tacaacaagc gatttatgat caagttttaa agacgacggt ggccatgttt
    46501 gcggaaaaat atacctatga agaaactacg attttgacac aagtccagca gcttcagcta
    46561 aaggcagtcg gaaaagtacc agtagatcta ggttggaaaa agctatttgg caaagagagc
    46621 gacagaaaag agaaggaaga agaaccgcta cttcctaaag taacaaaagg agaaacggtg
    46681 acggtagatc tacaaatctt tgaaaaagaa accaagccac cacaacctta cacggaaggt
    46741 acactaatca ccgccatgaa gacagctggt aaaaccgtgg atagtgaaga agcacaatcc
    46801 atattgaaag aagtggaggg aattggtaca gaagcgacac gagcaaatat tattgaaacc
    46861 ttgaagcaaa aggaatatat caaggtagag aaaaataaac ttgtggtgac gaataaagga
    46921 attttgcttt gtcaggctgt tgaaaaagaa ccgctgttaa caagtgccga aatgaccgcc
    46981 aaatgggaaa gctatcttac aaagattggt gagcaaaaag ggacacaagc tacttttcta
    47041 gcaaatatcc aaaaatttgt ttcccacttg ttggaagtcg tcccaggaca aatccaagcc
    47101 actgattttg gagcaacgtt acaagaggtg aaagtagcca gtgaaaagca agaagcggcg
    47161 cgtcatttag gatcttgtcc aaagtgccag gaaaaagaag tcatacttta tcagaaggtg
    47221 gcgacttgca cgtctgaagc ctgtgatttt aaattgtggg taacgattgc caaaaagaaa
    47281 ctcacagcga cacaactgaa agagatcatt cagaacggcc gaacaagtca accagtcaga
    47341 ggactaaaag gtcagaaagg ttcgtttgaa gcaacgatcg ttttaaaaga agactttaca
    47401 acaggatttg agttttctga aaagaagaag accacctaca aaagaaaagt gagacgtaaa
    47461 acgagttaga attgacggag ggaaaaacga tgagaaatac aacgcataaa gaacaagaaa
    47521 tcgaaaatca aaaagccaaa atacgttcag agcaggagaa aatgcgtgcc agcaaacgaa
    47581 gactacagca attgaagaat caacaacgga ttcaaaaaga acttgaatta gatcgataaa
    47641 tcctcctgtt gttaatatta aactaacatg ttatagttat gttagtttaa tattaacatt
    47701 cgagagggaa agcgatgaaa gataaagtca agtgcatttt tgagaaatac aatggtaatc
    47761 tatccttgaa ggatgctaga aatgaagggt tatcacctat gacgttagat cgtttagccg
    47821 aaaaggacaa aatagaaaaa atagcatcag gtttttatac cttaaaagat agttttgtgg
    47881 atgaattata tctcgctcaa agtatctata ctagaggaat cttctctcac gagacagcgc
    47941 ttgatctttt tcaagtagga acatatatcc ctaaaaaaat taatatgatg tttcctaaag
    48001 gatacaatgt ttcgaaagag aatttagatc agtatgccat tcaaccgcat tacacgaata
    48061 aagaaaactt tgaactggga atcacagagg cagaaagctt ttacggcaat aaaattttcg
    48121 tctatgattt agagcgaacg ttatgtgata tgtggaatcc cagatataaa gcaagcgtag
    48181 aagtaaaaca agaagcatta aaagaatata tgacaagtac cagcagaaat acaaataagc
    48241 taagaagata catgaatatt ttaaaatcac caaaagaaat gactttgtat atgttgccac
    48301 tttattgaga ggagtaaagg tgaatgacac cgacacaatt taaagcgaaa acaagaaatg
    48361 ttgcagctga gaaacattta aacccacaaa ttgttcaaag atattatatg atggaaaaat
    48421 tattagaatg tattgttgaa tcgccttatc atgatgattt tgtattaaaa gggggatttc
    48481 tgatcggatc aaaatacggg ttagaaaacc gttcaaccgt tgatattgat acgacttatc
    48541 gaaattcgaa actaacaaaa gaaaaattag aaactgtttt ttctgaatta acagctgctc
    48601 ctacaaaaga tggcattcgg tttgaattaa agggcttaac agagacgagg gaagccgatt
    48661 attacccagg ttttcaagca aagtttattg cttttcttga aaatgcacgt gtacctttta
    48721 aattagatat tacttcaggt gattctatta ttccagcagc attgatatat gaacataaat
    48781 tgatgtttga agataaaaca attcatattc cagcttatcc tacggaacaa attttagctg
    48841 aaaaattaca tgccacgttt agtttcggaa tagataattc acgtatgaaa gatttctatg
    48901 atctgtatat gatccctaaa ctagaacgta tcgatgagat aaatttgtat aagagtgttc
    48961 aaggaacatt taaggaaaga aataatcaat cagtgtttaa gaatttgtat aatcaggaaa
    49021 tgcctcaatt gatgaagaat gagggattga gggagaaatg gaaaagatat cagcaagaaa
    49081 actattttgc caaagatatt tctttcgaat ctaccttaac ttctgttgat tcgctgatgc
    49141 aatcaattgt cgagcaagag gaagtagaac gaaaacaaaa ttttaagaga tcacaacaaa
    49201 tgaatcaaga aatggagaga taaagatgat caataatgta actttagtag gtcgattaac
    49261 caaagatccc gatttacgtt acaccgcaag tggcactgca gtgaccactt ttaccttagc
    49321 cgttaaccgt aattttacta atcaaagtgg caatcgagaa gctgacttta tcaattgtgt
    49381 gatttggcgc aaaccagctg aaacgatagc gacattagct aaaaaaggca gcttgatcgg
    49441 cgtcgtcgga cggattcaaa cgcggactta tgacaaccag caaggacagc gtgtctatgt
    49501 gactgaagtt gtggccgata atttccaact attagaaagt aaagcagcga cagaaagccg
    49561 tatgcacgct gatcaatcgt ccagcagtcc aagccctacc acctttgaac aacgggatac
    49621 agcgacgcct aacaacaatg gattgaacgc ttcacagaat ccatttggtg gacaatcaat
    49681 cgatattagc gatgatgatc taccgttcta agtagcattt tctattggtt gcatttgcta
    49741 cggtttatgt tataatttag acaacaaaaa gaattttcaa tctataacca aaaaccacta
    49801 gctggcgggc tagtggcttt cttttacgtt tctattataa gaattaacgg gtaggcttac
    49861 tgtctttctt ggcaaattta caatcaagcc atttgcagat atagtgcgcg gctacttctg
    49921 ccatgattgc aaaaacaaaa tcaaaaagaa ttttcaaacc tataacctcc tccccttgcc
    49981 agattaggcc ggggtagcag acagcaagac tattatagca taattgaaat atccaaatga
    50041 ttggattttt gctataattt tagcgaggtg attgtgatga acaaatatgt tggtttgctt
    50101 gataaaatcc gagtaataaa aacgcaaccg ttattggttc gctttacttt acaaacgatt
    50161 catgaatcga ttaattgtgt agttgcagat attgaaatta tcgataaatt gctaattatg
    50221 gatgatggaa aatacaatat tgccgtcaca ggtcacttca ataaacgaaa tcagctagtt
    50281 attgctagca tgtatgttag aaatcccgat cattttacgc gatcgatggg catataaata
    50341 aaagaagagt tgtagtccta aatgaggatt gcagctcttt ttgttttaca tagaaacagg
    50401 aaaagacctt tttaggaaga cacaagtgcc ttctcaattg aaaaaccttt ctctcactcc
    50461 aaccgccacc gccgttcgag aagaaaggaa gtgaacgctg cacgttcact aagatatggt
    50521 gtcttgcaaa gcaagacaaa atagacaaaa cttcctctca ccaaaaggtt
//
LOCUS       DACDKM010000008        49683 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20834_2016
            SAMN12640370-rid9891663.denovo.008, whole genome shotgun sequence.
ACCESSION   DACDKM010000008 DACDKM010000000
VERSION     DACDKM010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640370
            Sequence Read Archive: SRR10040891
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 49683)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 49683)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742835.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742835.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:04:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,012
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 29 of 75
            Pseudo Genes (incomplete)         :: 33 of 75
            Pseudo Genes (internal stop)      :: 19 of 75
            Pseudo Genes (multiple problems)  :: 5 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..49683
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640370-rid9891663.denovo.008"
                     /isolate="E20834_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            139..1359
                     /locus_tag="IV334_000494"
     CDS             139..1359
                     /locus_tag="IV334_000494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair exonuclease"
                     /protein_id="HAP8942590.1"
                     /translation="MVKFIHAADLHMDRSFEGLTTLDKTVQEKLLKTNLTVLSNIIEQ
                     AIINNVDFVLLAGDNFHQNRPSLKIQKHFSEQMKRLEKNHIPVFIIFGNHDFYQKERY
                     WFSFPKNVHLFTSETVETKKITIKSGETVSLSGFSYRQPWIQKDKVMEFPSRELTDYH
                     IGLYHGEPGVSQKGNYAPFQPSKMQEKGYDYWALGHIHVPTVLNEKQTIVYPGAPQGH
                     TKKEQASTSILLVELSKGSCQIHPIKVAEVYWKTKEISLRKARTTKEVIVHIRQQLSK
                     VEDGFSLIEIKLKDYDHLNTDVLERIQSGELLDYLLEEFSDRINDFFIWRISLIENTF
                     YTKTPLAASAKLMEQLFQYYQTPQDFQQILNEVYSHQEAARILSELPEYQEETLEKAK
                     QLLNQDFLFEEDQE"
     gene            1356..4076
                     /locus_tag="IV334_000495"
     CDS             1356..4076
                     /locus_tag="IV334_000495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAP8942591.1"
                     /translation="MKILRIEIAAFGKWRQKSFDFYSGNQLIYGGNEAGKSTIYQFIQ
                     AILFGFPSKGRKKKDYTPKDGSAYGGKIWLKHPVYGEFAVERYKQQNRGKSKVWLGDQ
                     VGSDELLEKLIAPLTKELFQQVFTFQQEQLMELDHLREKELHDALISLGITGSSQVFQ
                     KRQEYYQKAQQLFKKKGQKLLINQKLNEWKELQEKIHQKQEQEAYFGKLIQQEQEYES
                     QQQQLLAQLKEANKQFSLARQQELNFSLYEEWQSLKKNSTTGLPDEQLLLDLESFSKR
                     YQQTNERLEELEATLEKHSGMDQQSARYYFYLEQEQELKDLQQLQFTAEQLNEEWQRL
                     QIETRETNSELKILEQRWHWQKNPLPEEFLYEEEWKHLLAEERRIADLESQISVRLQI
                     AKEQQEMIEAEVNDLEETYPALLQNQSEIAPVSSSKPYMLMAGGAIIIAGIFVPMPLK
                     VILLLIGAGMIGYFFYKRQPKIKTKNDFSEIKGMWQEKLGQLDLALAQLAEAEEKQKR
                     TGEEKQILQQEVYRFADHRHLGKMDTLKLMKEYGQEVIDYQKLYGVFERKHKREQEIQ
                     EKLRQIDQKFEFVKEWLPIQDKTISEKMVLLSRFIQEMEQMKFARSYQQNTLLKQEIN
                     QLKKKQKVWIQEYSEQLTQAGLSYPSEVPVFLQKAKQAEQQMKRYNELSEMLTPLFPE
                     ELTKEKLSVRLRQLKEKQSQLQQEEKEASENRQRLQLQVEELQRDGTLDELYQQASRQ
                     KAKLQELLEEWGALEIAGTFLGDLSTEMSERQLPQLLRAAGDYLGILTKGNYQKILLK
                     NDTLTLTDGYTEFPIYELSTGTKDQLIMAMRFAYLSLEANRSICPIIIDDGWLHYDHQ
                     RKYQLAELLAKFGEKYQIICFSSDKEMVSYYHEMNQIVKQLEGANNEKNS"
     gene            4060..5004
                     /locus_tag="IV334_000496"
     CDS             4060..5004
                     /locus_tag="IV334_000496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HAP8942592.1"
                     /translation="MKKIRDLVIDEQFEQFVLIKSADVRIAKNGKKFIAFTFQDTSGT
                     IDGKYWDASEEEINKFTAGTVVFLNGKREVYQGNPQVKILHMRLTRVDEPGEPSLYME
                     RAPLKKEEMEEEINQLLFEITNAHWNRIVRYLLNKYQKQFFEFPAAKRNHHAFASGLA
                     YHTLTMLHLGKSIADEYPELNRALLYAGIILHDLGKVIELSGPMSTEYTLAGNLIGHL
                     VLIDEEITKACLALKISEADEDVLVLRHMVLAHHGLLEYGSPVRPRIMEAEILHQIDN
                     IDASMQMMLTSIRQTEPGKYTDRIFGMDNRSFYVPKDL"
     gene            complement(5073..6083)
                     /locus_tag="IV334_000497"
     CDS             complement(5073..6083)
                     /locus_tag="IV334_000497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HAP8942593.1"
                     /translation="MKKKSIILAATSALAVLTLAACSGDTNKDIATMKGGTITVSDFY
                     DEAKLESSNQSLVQRMIIYKVFNNKYGDKVTDKQVDAEYDKQAKSLGDTFESQLEAAG
                     YTKDTYKEYIRNNLAFEAGLKAHVDITDDDLKTAWKSFHPEVEAQIIKLSSEDEAKDV
                     KKSADDGDDFSKLAKDKSTDTETKEDGGKVKFDSTTTTIPAEVKEAAFKLKDGEISDV
                     ITTTNPTSYATEYYVVKMVKNQNKGNDMDKYKDQLKDIATETKLSDNAFTTKVIGEEL
                     KDANVKIKDDAFENVLSAFTTTSSSTKDSSETTASTKSSDTKSTDSTKESSTEETTDS
                     SK"
     gene            complement(6263..6607)
                     /locus_tag="IV334_000498"
     CDS             complement(6263..6607)
                     /locus_tag="IV334_000498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HAP8942594.1"
                     /translation="MIKNFFKGLLFGAAAGTVGGLLLAPRSGNETRKKLIEELEEATT
                     LTNDLNNSLNHFKSALIETKETAESVIPAFQEAIKKDIEDFKFQAEPRITQINEQVEK
                     LKADLPEYPEQI"
     gene            complement(6600..7034)
                     /locus_tag="IV334_000499"
     CDS             complement(6600..7034)
                     /locus_tag="IV334_000499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HAP8942595.1"
                     /translation="MENCIFCKIINQEIPSYKIYEDDKVYAFLDISQATKGHTLVVPK
                     RHVADIFEYDPELAGEVFSRVPKIAQALEKAFPEMKGLNILNNNRELAYQSVFHSHIH
                     LVPRYSKEDDFSIHFVNHQDSYGSEELKAIQETIVKQVSCDD"
     gene            7172..7909
                     /locus_tag="IV334_000500"
     CDS             7172..7909
                     /locus_tag="IV334_000500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP8942596.1"
                     /translation="MSLIIDHVTGGYGHLPVLKDISFDVQPGEIVGLIGLNGAGKSTT
                     IKHIIGLLEASKGTIEINHLSLKKNTEEYRKQIGYIPETPSLYEELTLREHIEVTAMA
                     YDIPMDIAMKRAEKLLETFRLSNRLDWFPANFSKGMKQKVMILCAFLIEPSLYIIDEP
                     FLGLDPLAINALLELMVEMKKEGAGILMSTHILATAEKYCDRFVVLHNGEIRADGSLE
                     MLRKEFQLPDSSLDEIYIALTKEQAHE"
     gene            7902..9113
                     /locus_tag="IV334_000501"
     CDS             7902..9113
                     /locus_tag="IV334_000501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP8942597.1"
                     /translation="MSSFFQKRLARHQKKMMKYMRYVLNDHFVLVCLFLIGGVGLYYS
                     NWLKTLQPPFAIGGLIISVFWMLCLFVGKMATFAEAADIVFLLPKEKEMQTYLERAFR
                     YSCIFPLIFLILACGLAMPLVVVSTEQSFSMFFVYLIILWSLKFSHLQIKRAELFRLD
                     QQVIRYWKLGWFFSSLIFLFLSFYWLIWAGIAGALVQASLYYWFLWKQQEKGLDWEKM
                     VHVEEHRLHQIYQFINLFTDVPEITAKIKRRRYLDPILTHIKRESKNTYLYLFARRLA
                     RGSDFSGLYFRLVIIGGLLIAGVVDFYFSLIIGFLFLYLIGFQLLPLANQFRYMVLVQ
                     LYPIHKNQKKQAIQYLLTWVLIIASVIFGLIAAVVLDGQQRWVPLAAYLIVTMLFVKL
                     YVPTRLKKMSD"
     gene            9320..10099
                     /locus_tag="IV334_000502"
     CDS             9320..10099
                     /locus_tag="IV334_000502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase family protein"
                     /protein_id="HAP8942598.1"
                     /translation="MEFQLDQEWRLRPIKGDTGKTYIGMRDEDKVFIKRNTTPMLAAL
                     SREGITPKLVWTKRTGNGDTLTAQEWLEGRLLTPNEIGQRNDVIDVLYHLHHSNSLKT
                     MLTRIGGRVMTPEKMLQKYEKELPEELQKNQFLQIVHRFLREQIPSIDKENLSVVHGD
                     VNHRNWLVCKNYLYLVDWDSIMFADPAVDIATILGNYVPLSSWNQWLISYGIRPTDEM
                     LEKFHWYAAMNLLQEITRYCKREDHRRMNEEILQLKRIFSG"
     gene            10169..10831
                     /gene="trmB"
                     /locus_tag="IV334_000503"
     CDS             10169..10831
                     /gene="trmB"
                     /locus_tag="IV334_000503"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HAP8942599.1"
                     /translation="MRVRNRPGAAEMLAAHPNFVISDPTLWKGKWNELFENDHPIHIE
                     IGMGKGQFITGMAKAHPEINYIGVEMQVSVVSIALDKLIEQPLPNLKLLHVDGSALTE
                     YFADSEVDQIYLNFSDPWPKKRHEKRRLTYKTFLAVDEQILRPNGEIHFKTDNQGLFE
                     YSLASFSQYGMILKQVWLDLHQSQFEGNIMTEYEEKFSSKGQRIYRVEAQFQDKTKKK
                     SL"
     gene            complement(10929..12683)
                     /gene="ade"
                     /locus_tag="IV334_000504"
     CDS             complement(10929..12683)
                     /gene="ade"
                     /locus_tag="IV334_000504"
                     /EC_number="3.5.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine deaminase"
                     /protein_id="HAP8942600.1"
                     /translation="METTLRKYIEVASKKKPADLVIKHAKIVNVFTKEIMEKDVAICE
                     GMIVGIGEYEGKSVHNANGQYLVPGFIDGHVHIESSLLSPSEFSKVSLLHGVTTVVTD
                     PHEIGNIAGSTGLEFMIEDARQTLMNIFFMLPSCVPVTPFETNGANLNAKKLAPFLQK
                     PEVLGLAEVMNYQAVANNETDILEKIQLMHQHKKKIDGHAAGIGMEELNVYPAAGIRT
                     DHEATTAKEAKERLDLGMYLMVREGTVAKDLASLLPAITPENSRRCFFVTDDKLINDL
                     VKEGSIDHIVRQAIALGIDPLQAIQMATLNAAECFDLKTLGAIAPGYQADFFLTDDLT
                     KLPIHEVFYKGTLVVQSGQLQDHLFADHSNSYTCCLPKLNAQPLNPHSLELLLSSSQA
                     HVIEIIPNSLVTKDLVEKVDRQGERFIPSIEKDQLKIAVIERHHQTGNIGLGIVKGFQ
                     LKQGAIATTIAHDSHNLVVVGTNDEDMLYAANLLIQKGGGMTVVNKQQEIACVSLPIG
                     GIMTQEPFSIVYEQLNELITKAHQLGAAKTFDPFLTLSFLTLSVIPELKITDKGLFSF
                     SRFQLIPPCGKTDEINTF"
     gene            12815..13744
                     /gene="cpaF"
                     /locus_tag="IV334_000505"
     CDS             12815..13744
                     /gene="cpaF"
                     /locus_tag="IV334_000505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Flp pilus assembly complex ATPase component"
                     /protein_id="HAP8942601.1"
                     /translation="MEIKRLSDQMISESFAKHAQDVYILPASEVFYIYFRYGKKRTFW
                     KKVSSADGQQLISRFKYLGQMDIGEKRKAQLGAFTYSLEKQEVRLRLSSVGDYLQRES
                     LVIRLLYGTFETPHRCFSSRDFATILQKTVRRGLYLFSGTVGSGKTSLMYRLALEEKL
                     QVITIEDPVEIEEPFFLQLQINPKIAQTYDTLLKLALRHRPDLLIIGEIRDESTAHAA
                     VRAALTGHRVFATVHARNLEGTLVRIHELTKKKEELSECLAGVIYQELLEDANHHPSV
                     LWSYQFMEEESVKKSWEESYEEAQENIWQSELF"
     gene            13701..14750
                     /locus_tag="IV334_000506"
     CDS             13701..14750
                     /locus_tag="IV334_000506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein ComGB"
                     /protein_id="HAP8942602.1"
                     /translation="MKKLRKIFGSQNYSKGLTRSQQNQFAQTMGELLLSGFSLQESLY
                     LLLTIRSFPPVILANIQHQLKEGQAFSEVLSQIGYGAEQLVQIELAERHGNLPTTLLE
                     IAKQFRLVEGFRNDLKKLLAYPMLLLTFLLGILFSMRLFVLPQLIGSGMVMQEHWGIQ
                     LIQVFHWYVLLFILAVTLLALLVRYRLMKLSPVDQSEWISRQLLIGPIYSLYQSAYLS
                     LEIGKLFHEGFELRQIIVCLKETKQESLIQHLASKLIQGLEAGMSLAEQFQNYRFLSE
                     EFSKIVLQGEAKGSLGKELIFYSEWTRKQLFKRLHHWMHFIQPIIFLGVAALILLVYA
                     AVLLPVYGSIEEVLP"
     gene            14747..15025
                     /locus_tag="IV334_000507"
     CDS             14747..15025
                     /locus_tag="IV334_000507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HAP8942603.1"
                     /translation="MKKRKISYYSGFTLIEMLIVLLIISVLVLLFVPNLSRYRNHVDQ
                     ESREAIIQLVDTQKELYALQNNGRVPTVEELLNEGYIKREHAEIYQRP"
     gene            15000..15470
                     /locus_tag="IV334_000508"
     CDS             15000..15470
                     /locus_tag="IV334_000508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HAP8942604.1"
                     /translation="MQKSISGLEGKVLASGYTLFESLLLILLLTTILSFPVLAFSAWK
                     NELAVYQFFGQFEKRIYSTQKIAIVNQIQTGFYWNSEENQIIFHVPNPDKINWEVLDI
                     PDEITLKRHASITFAARTGNESSLKAYQFYWEEKERTITYQFQIGSGRYTKRIE"
     gene            15487..15789
                     /locus_tag="IV334_000509"
     CDS             15487..15789
                     /locus_tag="IV334_000509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8942605.1"
                     /translation="MESLTALMIASVCVYLVSFGQLFLLQAEKKSGEELLFIRMIYED
                     IKTQRMYHKRPERREEMIPYDIQIVDNNGVIEKVFVNMEKEAISIEKTSNSSIYAD"
     gene            15806..16177
                     /locus_tag="IV334_000510"
     CDS             15806..16177
                     /locus_tag="IV334_000510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComGF family competence protein"
                     /protein_id="HAP8942606.1"
                     /translation="MILSSLLLTFNLLIQQTIQVSEFLQRKDQKEWLIFLAQLEEELK
                     SSHNVSVKNQQLHYTIEDKQYIIERYQAMIRKRRTSGGHQPMLTSITELQLLEKEHTI
                     SFYVHFENGEDGYAIWTKNDQ"
     gene            16149..16508
                     /locus_tag="IV334_000511"
     CDS             16149..16508
                     /locus_tag="IV334_000511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8942607.1"
                     /translation="MPYGQKTISRKCYKGSILFSVLLLFLLCTFLLMAALESYRLSMD
                     LNIRIKNYYIAKIMKQMALSEQSTLAESSEGVFYYTTGSVTYQWVQQSLFLEVEVSPF
                     IFRFTEEVKNDTDTSTE"
     gene            16680..17687
                     /locus_tag="IV334_000512"
     CDS             16680..17687
                     /locus_tag="IV334_000512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAP8942608.1"
                     /translation="MLPEKMEQGFNQNLEAIQLLQNALGTSFLEAYVENAENLIDDYQ
                     VRVVDGVPTKETTQRITALYEELKKLSFEPEEWRRLSQLLLLKGSQTEHLQANHQLTP
                     DSIGFLFVFLIEQLYTNKKAPVKILDIAAGMGNLLLTVLLNLSNAGYQTEGIGVDIDD
                     TLLAVAASTSDLTQANVQYFHQDGLQELLIDPVDFAISDLPIGYYPNDEKAKEFLTST
                     EEGHSYAHHLLLEQSMKYVKPDGFGLFLMPSGFLETDQSEEIKKWFKEEGYLQGMIQL
                     PDELFRNKQSQKSILILQKKGPQAQQVKEVLLVKLASLKEPEKVTEFFNEFKNWKSSA
                     L"
     gene            17712..18896
                     /locus_tag="IV334_000513"
     CDS             17712..18896
                     /locus_tag="IV334_000513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HAP8942609.1"
                     /translation="MSKTIAINAGSSSLKWQLYQMPEETVVAKGIVERIGLQDSIFTI
                     KYGDNQKFEQIMDIENHEVAVKMLLDQLIELQILSSYDEITGVGHRVVHGGETYGDSV
                     VIDEKVMDRIAELAEFAPLHNPANLMGIKAFKKILPDILSVAVFDTSFHSTMPKQNYL
                     YSLPVSYYEDFKVRKYGFHGTSHRYVSERAAKLLNKPIEELKIITCHLGNGVSITAVD
                     GGKSMDTSMGFTPLAGVTMGTRSGDIDPAVLPYLMEKLEIDIDEMINVLNKKSGLLGL
                     TGLSSDMRDLEKNYEKEHIRLAYDIFVDRIRKYIGGYVTTMNGVDAIVFTAGIGENDG
                     HVRSEIIKGMSWFGCEIDPALNELRGEEVDISTKESKVRVLVIPTDEELMIARDVERL
                     RG"
     gene            complement(19333..20916)
                     /locus_tag="IV334_000514"
     CDS             complement(19333..20916)
                     /locus_tag="IV334_000514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HAP8942610.1"
                     /translation="MIIQAISIKKTIGGNPLFEDLSCQIEAGEKIALVDINGTGKSTF
                     LQILTGREGVDSGVISRKKGLKIGTVEQELTVSEATVNHYLLHSAAEIQDLKQQMSRY
                     EALMTEPDTNLEKCLARYGELQHRFEELGGYVLEDRIAAILQGLGIPHCQEARLTELS
                     GGERVKVALAKILAADADLLLLDEPTNHLDLNSIRWLENYLQNTKKSVLVVSHDRQFL
                     DQVTTKTWELEEGALIEYPGNYSRFRVLKEARLAELTKNYELQQKEVQRLKVMIRRFR
                     QWAHEGDNESFFKKAKELERRLAKLTLVKPPPPPKNRLQSLSNGGKSGKEVFIIQNLH
                     QQYADQVLFKDSSFAVYRGDHLAIIGDNGAGKSTLLKLLLGEEKPYGGTIKLGSSLQI
                     GYLPQQLQFADPDSRLLAYAITLTGNEEVARRQLAKSGFYQSDVGKRIKDLSGGEKIR
                     LALLKLFLEKINVLILDEPTNHLDSYAREEIEEMLQDYQGTLLAVSHDRYFLQQHFQE
                     ALVIDQEQISRQPLGQLID"
     gene            complement(21494..21637)
                     /locus_tag="IV334_000515"
     CDS             complement(21494..21637)
                     /locus_tag="IV334_000515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="portal protein"
                     /protein_id="HAP8942611.1"
                     /translation="MEVSDESDTKKYEVSMADTDFIAKLKFYFGFGYREKASATFGKS
                     FKN"
     gene            complement(21859..22338)
                     /locus_tag="IV334_000516"
     CDS             complement(21859..22338)
                     /locus_tag="IV334_000516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HAP8942612.1"
                     /translation="MLQQYKKIMVAVDGSDEAELAFKKAVNVAIRNNGELLLAHVIDT
                     RSFQTVSSFDGMLAEQATEMAKQTLADYESNAKKAGLNNVTSVVEYGSPKQIIAREIP
                     EDNQVDLIMLGATGLNAVERLFIGSVSEYVIRNAACDVLVVRTDLENQLPAKDHTKA"
     gene            complement(22411..22695)
                     /locus_tag="IV334_000517"
     CDS             complement(22411..22695)
                     /locus_tag="IV334_000517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8942613.1"
                     /translation="MIRILMIIATLVLLFVSYYLFNKQDIFFVLINKNDKNQGFLQFY
                     GAAYAVLGVMGILTAFFNQRFIALVFLLIVIIVSATFSIRFAKKIAEPKQ"
     gene            complement(22773..22967)
                     /gene="ssrS"
                     /locus_tag="IV334_000518"
     ncRNA           complement(22773..22967)
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="IV334_000518"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            complement(23039..24325)
                     /locus_tag="IV334_000519"
     CDS             complement(23039..24325)
                     /locus_tag="IV334_000519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HAP8942614.1"
                     /translation="MQQPLAYRMRPRTIDEVVGQQHLVGEGKIIRRMVDARMLSSMIL
                     YGPPGTGKTSIASAIAGSTNYAFRMLNAATDSKKDLQVVAEEAKMSGTVILLLDEVHR
                     LDKTKQDFLLPHLESGRIILIGATTENPYITINPAIRSRTQIFEVKPLTEQDIQLAVE
                     HALRDKERGLGQQAIQLDEDALLHLSRATNGDLRSALNGLELATLSTPSDKEGRIHLT
                     LSIIEECVQRKALTHDKNGDAHYDVISAFQKSIRGSDVDAALHYLARLVEAGDLASIC
                     RRLMVIGYEDIGLGNPAAAARTVNAVLAAEKLGLPEARIPLADVVVDLCLSPKSNSAY
                     MALDAALADIREGKAGDVPDHLRDSHYKGAKSLNRGVGYQYPHHFDQAWVNQQYLPDK
                     LKNAQYYQPKDTGKYEQALGQQYYRIKEWKKHPPKN"
     gene            24463..25134
                     /locus_tag="IV334_000520"
     CDS             24463..25134
                     /locus_tag="IV334_000520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase"
                     /protein_id="HAP8942615.1"
                     /translation="MTDKQKIDEFFWNKTTPEVARDLLGMYLEHDTPEGRLAGYIVDA
                     EAYLGPEDEAAHSYGLRRTPRVRAMYEKPGTIYLYTMHTHRILNIITQPEGIPQGVMI
                     RAIEPAAMIDQMSKNRGGKTGPDISNGPGKLVEALAIPQELYGQSIADSSLRLVFEKK
                     KTPKKINALPRIGIPNKGVWTEKPLRFVVSGNPYISLQRKNQIEKDWGWRKENEKEGS
                     INIFR"
     gene            25100..25588
                     /locus_tag="IV334_000521"
     CDS             25100..25588
                     /locus_tag="IV334_000521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3013 family protein"
                     /protein_id="HAP8942616.1"
                     /translation="MKKKDLLTYLDEIIEKQVTDYDVALDWDTKNHTIEIVVRLFAEN
                     KAQFEIDDAEGIVSQEPIIEFEDGVLLFNPQKSVFDERNYLAVIPYEGKKGLAKSVAD
                     SLVEYLNEVLAQGQSDLLDFLDEDDDEAVFELHWDNQQLAELVEVKQQNEADTYLPYP
                     SY"
     gene            25601..26548
                     /gene="prmA"
                     /locus_tag="IV334_000522"
     CDS             25601..26548
                     /gene="prmA"
                     /locus_tag="IV334_000522"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HAP8942617.1"
                     /translation="MKWNEVKVETESEAVEAVANILMEAGASGVAIEDSLDVEHFQSD
                     QYGEILDKNDFTSLKEGALVMAYFPETTFLPEILPFIKDRITQLPEFGLAIGKNKVTV
                     SEVEESNWATAWKKYYHPVRITRMLTVAPSWENYQTEDPLEKIITLDPGMAFGTGTHP
                     TTSLTLTALETVLRGGETLLDVGTGSGVLSIAAKYLGAKDVYAFDLDEVAVRSAKENM
                     DMNEIAKDVHVSANDLLTGIEIESDVIVANILADIILLMIPDAWRLLKQTGTLIVSGI
                     IEEKKQMVLDAMLEQGFIVDQIFQQKDWYAIILKKPEVE"
     gene            26549..27313
                     /locus_tag="IV334_000523"
     CDS             26549..27313
                     /locus_tag="IV334_000523"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HAP8942618.1"
                     /translation="MQRYFLKESYESKDYFVLEGENYHHIVRVMRMEPEDEVFLAFND
                     QIAIVAEITEITEEAVYLKEIKKEENQKELPSSITIASGYPKGEKLDWIVQKSTELGA
                     QAFLGFPASASVVKWDAKKRMKRQQRLEKIAQEAAEQSHRQIVPSVELLETKQDLFER
                     LDDYDVVLVAYEESAKQGEKSNAAKILSETKPDSRILAVFGPEGGLSRDEVDYFVEHK
                     GILCGLGPRILRTETAPLYLLSAASYQWELMNPSFS"
     gene            27546..29759
                     /locus_tag="IV334_000524"
     CDS             27546..29759
                     /locus_tag="IV334_000524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010749038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HAP8942619.1"
                     /translation="MTKEEILTGPGVIKLVSQYMGPEHVAFVEKACEYATAAHDGQFR
                     KSGEPYIIHPIQVAGILADLKMDPHTVATGFLHDVVEDTEITLEDLREEFGDDVAMLV
                     DGVTKLGKIKYKSHEEQLAENHRKMLLAMAQDLRVIMVKLADRLHNMRTLKHLREDKQ
                     RRIAQETLEIYAPLAHRLGISRIKWELEDTALRYLNPKQYYRIVHLMQTKREEREKYV
                     SGTVEDIRIATEELGIFAEIYGRPKHIYSIYRKMKDQKKQFNEIYDLLAIRVIVDSIK
                     DCYAVLGAIHTKWKPMPGRFKDYIAMPKANMYQSLHTTVIGPAGNPVEIQIRTQEMHE
                     IAEFGVAAHWAYKEGKNEKVEPDGMTKQLSWFHEILELQDESYDASEFMEGVKGDIFS
                     DKVYVFTPKGDVTELPKGSGPLDFAYSIHTDIGNKTTGAKVNGKMVQLDYKLKNGDII
                     EIMTSPNSFGPSRDWLKLVATSKARNKIKRFFKAQDREENVIKGHESVVKCITDLGFT
                     PKDILTKNKLQEALDRFNYQTEDDLYAAVGYGEVSPLTMANRLTEKERKEQKIEQQKQ
                     EAEEIMNQPKKEPDKMKVRHEGGVVIQGVENLLIRISRCCNPIPGDDIVGYITKGRGI
                     SIHRRDCPNVQPDKPNVAERLIEVEWEDTSNTRKEYDADLEIYGYNRSGLLNDVLQTV
                     NALTKNLNSVEARTNKDKMATIHLTVGIQNLSHLKSIVDKIKAVPDVYSVRRTNG"
     gene            29770..30216
                     /locus_tag="IV334_000525"
     CDS             29770..30216
                     /locus_tag="IV334_000525"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-tyrosyl-tRNA(Tyr) deacylase"
                     /protein_id="HAP8942620.1"
                     /translation="MRAVVQRVSKAAVSVDQQEIGKIGYGLLVLLGIHETDTQKDVDY
                     LVKKIAQMRIFEDEQGKMNISVEDVGGQILSISQFTLFADTKKGNRPSFVEAARPETA
                     IPLYESFNEGIRSRGITVATGQFGADMSVSLVNDGPVTIIIDSKNK"
     gene            30387..31646
                     /locus_tag="IV334_000526"
     CDS             30387..31646
                     /locus_tag="IV334_000526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HAP8942621.1"
                     /translation="MKEVPVVDIKITDLKKLYQKGYNMLEELRHEADAPVVKAKIFNK
                     EAITIYGSSAAKVFYDPRNFKRKGAMPKLVLKTLFGQGGVQTLDGAAHHHRKNIFMDL
                     MTPERMEDYHRILDKNLTQALEAQHGQFELFDLSKMVFFTSICEWAGINLSAISKDEV
                     EKLAEYQISMISGTFTSPIDHIKGVENRKKSEKWAQGLIEEARQNPVAGKENVALYAF
                     ANATDLDGQLLPLEVAAVELLNIIRPTVALTVWAALMGHALFSRPDLYQQLKNDFSTL
                     QDPFIQEMRRYYPFFPMLPAISLKEVEVDGYRIPEGSWVILDLYGTDHDERTVEAPDS
                     FMIKRYVGKAKDISYKEEYEMIAQGGGNFRQMHRCAGEWITLHSLRVFSDQLVNKFEF
                     SVPEQDWTIPFNQFPTYPNSRALLYKN"
     gene            complement(31735..32682)
                     /locus_tag="IV334_000527"
     CDS             complement(31735..32682)
                     /locus_tag="IV334_000527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HAP8942622.1"
                     /translation="MAEIRIGHSQVYAEQLGLGANAVGGHNLFDGLEDETGKQVVRTA
                     LNSGINLIDTAYAYGNGRSEELIGEVLKEKEYDRSRVVIATKAAHVPNKGRTFDNSPE
                     FLKQSVEDALKRLQTDYIDIFYIHFPDESTPKNESVATLHELKEAGKIRAVGVSNFTL
                     EQLKEANADGYVDVVEDKYSLIHRQAEKELFPYLEKNKISFVPYFPLASGLLTGKYEL
                     GEEKQFGEGDPRKRNPDFQGERFREILTAVDVLRPIAKRYQATPAQLVLAWYMKNPRV
                     SVVIPGAKRPEQVSDNVQALDLHLSNEDYQTIDEAFRGF"
     gene            33076..34368
                     /locus_tag="IV334_000528"
     CDS             33076..34368
                     /locus_tag="IV334_000528"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HAP8942623.1"
                     /translation="MSFQRPKGTNDILPGESEKWQFVEETARLLFRDYQYNEMRTPIF
                     EHYEVISRSVGDTTDIVSKEMYDFYDKGERHVTLRPEGTAPIVRSFVEHKLFGPEYAK
                     PFKTFYMGPMFRYERPQKGRLRQFHQIGVEAFGSENPATDVESMVMALDFFKQLGITQ
                     IRLVINSLGDKETRQAYRQALIDYLLPFESELSEDSKRRLHENPLRVLDSKDKRDQKF
                     VAEAPSILDYLSEPAQKHFDTVVSMLDALAVPYEIDSNMVRGLDYYTHTIFEIMSDAP
                     KMGAQATICAGGRYDNLVEELGGPSTPGFGFAMGIERLLLTMEAEEVVIPAFNELDAY
                     VVALGDETNIEALKVVQAIRNFGFSADRDFMNRKAKAQFKTADKANAKLVLVIGEDEL
                     ASQTINVKAMASRKEKSFPLEEIYEHFDEVYDEMTLFE"
     gene            34383..36152
                     /gene="aspS"
                     /locus_tag="IV334_000529"
     CDS             34383..36152
                     /gene="aspS"
                     /locus_tag="IV334_000529"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016251448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HAP8942624.1"
                     /translation="MAQRTIYCGLVSEQQLEQTVTLKGWVQKRRDLGGVIFIDLRDRE
                     GIVQIVFNPEINKEAWEIADKCRSEYVIEVTGEVRRRGENAVNPKMATGEFEVIASEI
                     VILNQAKTPPFQIEDDQAISDEIRLKYRYLDLRRPKMTKNIKLRNATTKAIRHYLDDN
                     DFLDIETPYLGKSTPEGARDYLVPSRVHAGHFYALPQSPQLFKQLLMNAGFDRYYQIV
                     RCFRDEDLRGDRQPEFTQVDIETTFLTAEEIQTYTEGLIAKVMKEVRGIEVTLPFPRM
                     TYDEAMARYGSDKPDTRFDMELIDLSDTVKEVEFKVFQMALENGGVVKALNAKGAADR
                     YSRKDMDQLGQYVGQFGAKGLAWLKVEEDGLKGPIAKFMGEATEAIIKATDAKPGDLL
                     MFGADKSEIVAAALGAIRTRLGKELGLIDESKFNFLWVTDWPQFEFSEEEGRYVSAHH
                     PFTMPKEEDIPLLAEDPAKVYAEAYDIVLNGYELGGGSIRIHTRELQEKVLETLGFTK
                     EQMEEQFGFLLTALDYGFPPHGGIALGLDRFAMLLAGEDNIREVIAFPKNGKAADVMS
                     DAPSMVSPLQLFELNIDVTNIEE"
     gene            36261..37136
                     /locus_tag="IV334_000530"
     CDS             36261..37136
                     /locus_tag="IV334_000530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HAP8942625.1"
                     /translation="MKKTFENWAYHDYTTKLSVSIVYALIASVALNFFYQPGHIYSSG
                     ITGLAQILTTLSKSLIGFEVPVSTTLYLLNIPLFVLAWLKIGKKFTIFTFITVTLTSL
                     CIHILPETVLSTDPIICALFGGAVMGLGIGLALRNGLSSGGLDIVSITIRKKTGKSIG
                     SISIYFNALIVFVAGILFGWQYMFYSTLSIFVSGKVTDVVYTKQKKMQVMIVTKHPEK
                     VVNEIQNCMRRGITIINEAEGAYKHDKQTVLMTIVTRYELPLLREAMKASDPQAFVSI
                     AENVQILGRFYEDGI"
     gene            37402..39285
                     /locus_tag="IV334_000531"
     CDS             37402..39285
                     /locus_tag="IV334_000531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HAP8942626.1"
                     /translation="MEKQKRLKNSRYITGFDGLRSLAVVGVIFYHLLPTSMKGGYLGV
                     PIFFVVSGYLITDLLRQEWEQNGKINIWQFYVRRMKRLYPALVFMLVTASAYITLFQR
                     GLLNNLRGVVVSSLLYVNNWWQINNGLSYFDRFANESPFTHIWSLAVEGQNYLVWPIL
                     FVLLMVFVRKRKWIFYTVLGTSFLSAILMMILYTPGGDPTRVYYGTDTRLFSIWLGSA
                     LAFVWPSTRLKKNIPKQAKNILNIAGGISLLALILCFFFLDDHYNFIYYGGMLLVSLL
                     CVVLVAVTAHPGASLNRWLTNPVFTWIGKRSYGIYLYQFPVMIFYEAKVTDIADHVLL
                     HTLVEITLILIISELSYRYVERPLARFDYGRTFQVMKGWFSKPIISKSKPWVVPGTLL
                     VAIALVGFVTAPKNAVTADQKQLQAKIAESKKLAEETRNNSENTTSSVDQAILDKYEL
                     SKAQGNKAQKLELTAFGDSVMLDAATDLQELYPKVVVDGDVGRQLYASVPYIEKLKEQ
                     DLLKDTVLIGLGTNGSFSEAQFDSVMDAIGDRKVYWVNVRVPTKRWQNDVNAMLEQMT
                     KKYDNMTLIDWYSYSNDHSDWFYDDQVHPNPEGMTHYIHLVSEALLGGSSSTNSSETN
                     VTE"
     gene            complement(39360..39644)
                     /locus_tag="IV334_000532"
     CDS             complement(39360..39644)
                     /locus_tag="IV334_000532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HAP8942627.1"
                     /translation="MANEHYFYVLFCKDQTFYGGYTTDLSRRLEEHNNGTGAKYTRLQ
                     KRRPVKMIHAEVFSSRSEATKAEAAFKKLTRKQKERYLQTHSTVLLPESI"
     gene            complement(39634..40368)
                     /locus_tag="IV334_000533"
     CDS             complement(39634..40368)
                     /locus_tag="IV334_000533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA1(Val) (adenine(37)-N6)-methyltransferase"
                     /protein_id="HAP8942628.1"
                     /translation="MLHEDERIDQLYAEDIKIIQSSKVFSFSLDAVLLAHFSRVPKKG
                     KIVDLCAGNGAVGLFLSKRTQAAIDAIELQPRLADMARRSIALNHLNEQMTVHTIDLK
                     DSLSVVRHNSCDLVVCNPPYFKGLPTNKTNPNKHLAIARHEIHTTLEEVIDISSKLLK
                     TNGRFTMVHRPDRFLEIIQVMEDYRIAPKRIQFVYPKPGKEANILLVEGIKEGKLDGF
                     KVAPPLITYNMDGEYTAEIREMLYGK"
     gene            40497..41120
                     /locus_tag="IV334_000534"
     CDS             40497..41120
                     /locus_tag="IV334_000534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HAP8942629.1"
                     /translation="MFYRFMRGVVRVLLALVNGNARYENKEVLPQDENYILVAPHRTW
                     WDPLYLAVAAAPKEFSFMAKEELFKNPVLRFILTRSNAFPVKRENPGPSVIKTPVKIL
                     KSTNLSLIMFPSGTRHATELKGGMALISRMSKAKIVPAVYQGPLTLGDLFKRQRVTVR
                     FGEPIDLSDIKKTDKEGMAEIERRTQAAFDKLDKEVNPDFKYEVKKK"
     gene            41312..42434
                     /locus_tag="IV334_000535"
                     /pseudo
     CDS             41312..42434
                     /locus_tag="IV334_000535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628777.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease SbcCD subunit D"
     gene            42441..45569
                     /locus_tag="IV334_000536"
     CDS             42441..45569
                     /locus_tag="IV334_000536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMC family ATPase"
                     /protein_id="HAP8942630.1"
                     /translation="MKPKKLQLKNFGPFINETVDFSRLTEAPLFLISGKTGAGKTTIF
                     DGITFALFGETSGRLRSGKEMRSLFATPDEETSVTFSFEHQQMSYEIERKPEQVLAKR
                     KGNGTKKQAAKVTLTIFDGKGKEIKQITKRTEADQLIRELMNLDAKQFSQIVLLPQGE
                     FRNFLVSSSSDKETVLRHLFGTQFFQQFNERLKEKAKQQQQKLDHLEQELHLLQKRFV
                     LVKEEEPAAGFEAVLAQWTNHQAILEKQITEEKEALTELQEKQKQLENSYYRFESLKK
                     SYEEKTILINKQKELAAQKDEIEEKKRWIGYYEFTQRLVEPIQRLQEIKVEKIELSQK
                     SSEQTKKLAMTTQNYEEWQKNEANRVRRQTKIKEAAQKLQNKQMMVPIVESLRSKKQD
                     AEQLTISLDKEELNAEKLYQNLAIYQERTRILQENLLDQEKLQEDRLAFGQLKYLDEK
                     QQEIEKQKAKDTGSLAQIREELKKATEEITKNSQLLKEQTEQAEQLKSQWAKQQIARL
                     QLFLRPGEPCLVCGSTEHPIQQLKHEQPNKEDILKVEQQLAEAESAVERTQQIIAKSE
                     AHENNLQQQLSDLEQKIMATNIQLAEQEEHLQQALKKAFPEITEQKTKTTLVVIEQSL
                     DRRKSKMANERIELEEIRQQAEKLEQQLAVYKQQQTEKNNQLQQLQGEIKSLEQQLQD
                     TDSNCLEEEIRRLEKQLEEDSNQLAVEQKAGVQLLQDLHTLRTQLDLQHEQIEKIDEK
                     IEKEQERIAKKLESQSYFSTIEEICAFAQQQAQYKENQRTVTSYHEEQLVTADRLRQL
                     ENIQLTETFPDTEEISQQLEEIRRMIEIKQEKIYGLQEQKISNQKIYDECLSIYHTSQ
                     TKLDELTQLQQLSQTFNGENPKKTSLERYVLQVYLQEVLQVANDHLQRLTKNRYQFEL
                     ADTIGSYRGKTGLEINIYDDQAGMSRSAHTLSGGESFIAALSLALALADVIQTQAGGV
                     AIDALFIDEGFGSLDEEALEMAMEALETIESEGRMIGIISHVRELKERVLQQIRIDTE
                     GSGQSKIRYQLG"
     gene            45588..48782
                     /locus_tag="IV334_000537"
     CDS             45588..48782
                     /locus_tag="IV334_000537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HAP8942631.1"
                     /translation="MKWSIPEKVIARGRQYLQEGRVLSVVPDQEENVWHAEVLGSELY
                     MVDLDGTAKEIDYCQCPYWEEHKYCKHTVAVELYLRSKNLSRIMKKDQPMLEPVKATS
                     EGEIIAKGFSRLKTQNNPQAVQPLIIEYQVETIETNQYHPELSILALYLRIGSLASPK
                     KTYIVKNIYDFLQAYTEKETYTVNKQYQFRLDKRAFQKEDQKILTHLAGSAQTHQLLG
                     TNGLQVKGKLDKRYLLLPVEQARFLLEQMNQTTRLHLQINEQNDQGIQFSDQNKPLSF
                     KVEKFGEDYLLQALNDFDFFFMHYQWGVIQGTFYSLTKYQQTVYQTWKQLIRRLEKPE
                     VVFKKKELPSLFKEIIPLLSEIGDVAIAPAVEEEVADIPVEFIFFFRKAKGKIQARVD
                     FVYGEVIYSTDPKHEVSTEQAFQVLRDTVEEKRVLDLFRMYRYQKNETGYERPLPSGE
                     ELYAFFRTELSVFRQFGEVRLGRKLRELYLDAHKHQPKIEVTETGSWLDIRFDVTGIE
                     EQEIDHVLQSLLRNDAFYTLENGQVLSFDSEEFQQTSQVLQQLRESIRTEEGTIHVPK
                     NQGLIIQNQLEKSNATFSESFQTMVQDLIHPERYQAQLPKGLNATMRDYQKQGFRWLK
                     MLGHYQFGGILADEMGLGKTLQTIAFLLSEKEERKSFSALIVAPASLIYNWQAEVRKF
                     APSLSIQVINGNKKEREELLAKDTDIRVTSYASLRQDLANYQSQKIDYLILDEAQMVK
                     NSSTKTAQALRELAVPQRFALSGTPIENNLEELWSLFATIMPGFFPTKTKFRELAAEE
                     IAQMIRPFILRRDKQTVLKDLPEKTEMNLYSALTEEQKTVYLAYLRQMREEITSMDSE
                     AFKKNRIGILAGLTRLRQICCDPRLFIEDYQGGSGKLEQVKDLLVAAKENKRRVLLFS
                     QFTGMLTILQEELAELGISTFYLRGSTKPQDRLSMVDAFNAGEKDVFLISLKAGGTGL
                     NLTGADTVILYDLWWNPAIEEQAAGRAHRIGQKNVVEVWRMISEGTIEERMDSLQQEK
                     RELFQKVIQGNEEQLTKLTEEDIRMILSVGEIEE"
     gene            complement(48928..49635)
                     /locus_tag="IV334_000538"
     CDS             complement(48928..49635)
                     /locus_tag="IV334_000538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HAP8942632.1"
                     /translation="MIKSVVFDVDDTMYDQQQPFRNAVKRVVPLVSDADMHPLYIRFR
                     HHSDENFPKVMAGDWTLEYMRAHRISQSLKDLDYPHITEEDGLLFQKIYEEELDNICL
                     HEEVKKTLDFLKEKNVPLGIITNGPTDHQTKKLKQLQLNNWIPSRNMLISQATGFQKP
                     EKEIFQLAEKEFHMLPEETLYVGDNYDNDVLGAKSANWQALWFNHRERKIEQTPICDI
                     EISSFDQLLETMKTIFV"
BASE COUNT        16186 a         8761 c        10389 g        14347 t
ORIGIN      
        1 ctttttttcg aataaactta ttttttgaag tcggccattt atcgtaattt ctttgcataa
       61 tacgaacaaa ttttcggtta ttgatgaaaa aaaacattga aaatggtatg ataaaagaaa
      121 aaaaggagga aatttttaat ggtgaaattt atccatgcgg cagatttaca tatggatcgg
      181 tcatttgaag gactgacaac tcttgataaa actgtacagg aaaaactatt gaagacgaat
      241 ctgaccgttt tatcaaatat cattgaacag gcaataataa ataacgttga ttttgtttta
      301 cttgcaggag ataattttca tcaaaatcgt ccttcattaa aaatacagaa acatttttca
      361 gaacagatga aacggttaga aaagaaccat atacctgtat ttataatatt cggaaatcat
      421 gatttctatc aaaaagagcg gtattggttc tcctttccta aaaatgtcca tttatttaca
      481 tccgaaacgg tagagacaaa aaaaataacg ataaaaagtg gagaaactgt ctctctcagt
      541 ggatttagtt atcgtcaacc gtggattcaa aaagacaaag tgatggaatt tccttctaga
      601 gaactcacag attaccatat tgggttgtac cacggggaac ctggcgtctc tcaaaaagga
      661 aactatgctc ctttccaacc ctcaaagatg caagaaaagg gatacgatta ttgggcttta
      721 gggcatatcc atgtgccaac ggtcttaaat gaaaagcaaa caattgttta tccaggtgcg
      781 cctcaaggtc atactaaaaa ggaacaagct tctacatcta ttctactagt tgaattaagt
      841 aaaggaagtt gccaaataca tccgattaaa gtagcagagg tctattggaa gacaaaggaa
      901 atatcattga ggaaagcaag aacgacaaaa gaggtcattg ttcacattcg tcagcagctt
      961 tcaaaagtag aggatggatt tagtttaatt gagataaaat taaaagatta tgaccatctc
     1021 aatacagatg ttttggaacg gatacaatca ggagagctat tagactattt gttagaggaa
     1081 ttctcagata ggataaatga tttctttatt tggcgtattt ctctcataga gaatactttt
     1141 tatacgaaga ccccacttgc tgcttctgct aaattgatgg aacagttatt tcaatactat
     1201 cagacacctc aagatttcca acaaatttta aatgaagtat atagtcatca agaagcggct
     1261 agaatattga gcgaactgcc ggaatatcaa gaagaaacac ttgaaaaagc caagcagctg
     1321 cttaatcaag attttttatt tgaggaggat caggaatgaa gattttaagg atcgagattg
     1381 ctgcttttgg aaaatggcgt caaaaaagtt ttgactttta ttcaggcaat cagctgatct
     1441 atgggggaaa cgaagcagga aaatcaacga tttatcaatt tattcaagca atattatttg
     1501 gtttcccttc aaaaggccgt aaaaaaaagg attatacacc aaaagacggc tcagcctatg
     1561 gcggcaaaat atggcttaaa catcctgtat atggtgaatt tgctgtcgag cgctacaagc
     1621 agcaaaatcg cggaaaatcg aaggtgtggc tgggggatca agtgggaagt gatgagttac
     1681 tagaaaaact gatcgcacct ttgacgaaag agttattcca gcaggtattt actttccagc
     1741 aagagcagtt gatggaacta gatcatttga gagaaaaaga gctacatgat gcgctgattt
     1801 ctttaggcat cacgggcagt tctcaagtat tccaaaagcg gcaagaatat tatcagaaag
     1861 cacaacaatt attcaagaaa aaaggccaaa aattactaat caatcaaaaa ttaaatgaat
     1921 ggaaagagct gcaagaaaaa attcatcaaa aacaagaaca ggaagcttat tttggcaagt
     1981 tgatccaaca agaacaggaa tacgagtcgc aacagcagca gttattggca caattaaaag
     2041 aggcgaacaa acaattttca ttagccagac agcaagaact taatttttct ttatatgaag
     2101 aatggcaatc tctcaaaaaa aacagcacta caggtcttcc tgatgagcaa cttcttttag
     2161 atctggagtc tttctccaaa cggtatcagc aaacaaatga gcgtttggaa gaattagaag
     2221 caacactgga aaagcatagc gggatggatc agcagtcagc cagatattat ttttatttag
     2281 agcaggagca ggaactaaaa gatctccagc aacttcaatt tacagcggaa caattaaacg
     2341 aagaatggca aagattacaa attgagacta gagaaacaaa ttcagaattg aagattttgg
     2401 aacaaaggtg gcattggcag aaaaatccgt taccggaaga atttttatat gaagaagaat
     2461 ggaagcatct tctggctgaa gaaagaagga ttgctgatct agaaagccag atttctgtgc
     2521 gtctccaaat agctaaagaa cagcaagaga tgattgaagc agaagtgaat gacttggaag
     2581 agacctatcc agcattatta caaaatcagt cagagatagc accagtctct tcatctaaac
     2641 cgtatatgct gatggctggc ggagcaataa tcattgcagg tatatttgta ccaatgccct
     2701 tgaaagtgat ccttttacta attggtgcag gcatgattgg ctatttcttc tacaaaaggc
     2761 aaccaaaaat taaaactaaa aatgatttct ctgagataaa aggaatgtgg caagaaaaac
     2821 taggacaact tgatctagcc ttagctcaat tagctgaagc agaagaaaaa caaaagagaa
     2881 ctggagaaga aaaacagatt cttcagcaag aagtatatcg ttttgcggat catcggcact
     2941 taggaaagat ggatacgctc aaactcatga aagaatatgg acaggaagtc atagattatc
     3001 agaaattata tggcgtattt gagcggaaac ataaaagaga acaggaaata caagaaaaac
     3061 tacgacaaat cgaccaaaaa tttgagtttg ttaaagaatg gttaccgata caagataaga
     3121 caatttcaga aaaaatggta ttgctttccc gttttataca agagatggaa cagatgaaat
     3181 ttgccagaag ctatcagcaa aatactttat tgaagcaaga aatcaatcaa ttgaaaaaga
     3241 aacaaaaagt atggatacaa gaatacagcg aacaattgac tcaagcaggc ttatcttatc
     3301 cttcggaagt acctgtcttt ttacaaaaag ccaaacaagc cgaacagcag atgaaaagat
     3361 acaatgaatt atcggaaatg cttactccac tatttcctga agagctgaca aaagagaaat
     3421 tatctgtaag actgcgccaa ctgaaagaaa agcaatccca actccagcaa gaagaaaaag
     3481 aagcaagtga aaatcggcaa cgactccaac ttcaagtaga agagttgcaa agagacggca
     3541 cattggacga actttatcaa caagctagcc ggcaaaaagc aaaattacag gaattactgg
     3601 aggaatgggg agcacttgaa atcgccggga cattcttagg tgatctttca acagagatga
     3661 gtgaaagaca attaccccag ttgttaagag cagcaggtga ttatttaggt attttaacaa
     3721 aaggaaatta tcagaagata ttactgaaaa atgatacact gactttaaca gacggatata
     3781 cagaatttcc catctatgag ttatctacag gtacgaaaga tcagctgatt atggcgatga
     3841 gatttgctta cctctcatta gaagctaacc gttcgatatg cccgatcatc attgatgatg
     3901 gctggcttca ttatgatcat cagcgaaagt atcaattagc tgaactttta gctaaatttg
     3961 gagaaaagta tcaaataatt tgtttttctt ctgataaaga aatggtaagc tattaccatg
     4021 agatgaacca aatcgtaaaa caattagaag gagcaaataa tgaaaaaaat tcgtgattta
     4081 gtaatcgatg agcagtttga acaatttgtc ctgatcaaaa gtgcagatgt aagaatcgct
     4141 aaaaacggaa aaaaatttat tgcttttact ttccaagata cttcaggtac tatcgatggt
     4201 aaatactggg atgcttcgga ggaggaaatc aataaattta cagccggcac agtcgttttt
     4261 ctaaacggta aaagagaggt ttatcaagga aaccctcaag taaagattct tcatatgcgc
     4321 ttaacaaggg tagatgagcc aggagagcct agtttataca tggaaagagc ccctttgaag
     4381 aaagaagaaa tggaagaaga aatcaatcaa ttgttgtttg agatcaccaa tgcgcattgg
     4441 aatcgtattg ttcgctatct acttaataaa tatcaaaaac aattttttga gtttccggca
     4501 gcaaaaagaa accatcatgc atttgccagt ggattagctt atcatacttt gacaatgctt
     4561 cacttaggga aatcaatcgc cgatgaatat cctgagctga atcgagcatt gttgtatgct
     4621 ggtatcattt tgcatgattt aggaaaggtg attgaacttt ccggtcctat gtcgacagaa
     4681 tatacgcttg caggaaattt gatcggtcat ctggtattga ttgacgaaga aataactaaa
     4741 gcctgtcttg cgttgaaaat cagcgaagct gacgaagatg ttcttgtatt acgtcatatg
     4801 gttttagcac accatggctt attagaatat ggctcacctg tacgtccaag aataatggaa
     4861 gcagaaatat tacatcaaat cgacaatatc gatgcttcga tgcagatgat gttgacttcg
     4921 attcgtcaaa cagaaccagg aaaatacacc gatcggattt tcggtatgga caaccgaagt
     4981 ttctatgtac caaaagattt atagcaaaaa tttatttttt tcattcaaaa caaaaaaagc
     5041 gagacataag tcttcgcttt ttttgttttc ttttattttg atgaatcagt tgtttcttca
     5101 gttgaagatt cttttgttga atcagttgat tttgtgtcag aactctttgt tgaagctgta
     5161 gtttcacttg agtctttcgt acttgatgaa gttgttgtga atgcactcaa cacattttca
     5221 aaggcatcat ctttgatttt tacatttgca tcttttaatt cttcaccgat tactttagtt
     5281 gtgaatgcat tgtcacttag tttagtttca gtagcgatat cttttagttg atctttgtat
     5341 ttatccatgt cattgccttt attttggttt ttcaccattt tgacaacgta gtattctgtc
     5401 gcataagaag taggatttgt tgtagtaata acatcggaga tctcaccgtc tttcaatttg
     5461 aaagctgctt cttttacttc tgcaggaata gttgttgtcg ttgagtcgaa tttaacttta
     5521 ccgccatctt cttttgtctc tgtatcagtt gatttgtctt tggctaattt agagaaatca
     5581 tctccatcat ccgctgattt tttcacatct tttgcttcat cttcacttga taatttgatg
     5641 atttgtgctt ctacttctgg atggaaagat ttccaagcag ttttcaaatc gtcatctgta
     5701 atatctacgt gagctttcaa gccagcttca aaagctaaat tgttacgaat atattcttta
     5761 taagtatctt tcgtatatcc agcagcttct aattgtgatt caaaagtatc acctaatgat
     5821 ttcgcttgtt tgtcgtattc tgcatccact tgcttatcgg ttactttatc gccatatttg
     5881 ttattgaata ctttataaat gatcatccgt tgtactaagg attggttcga tgattcaagt
     5941 tttgcttcat catagaaatc agatacagtg atcgttccgc ctttcatcgt tgcgatatct
     6001 ttattagtat cgcctgaaca agctgctaga gttaaaacag ccaacgcact agtagctgcc
     6061 aaaataattg attttttttt cattaaaatt tgcactcctg taattagtta ttatttttat
     6121 atattaacta taccatatcc catttccttt aaaaacgttg tcgtggtagt ttcaaacaaa
     6181 attcaaaatt tcatcacatt cctaggaaat acgcaaactt ttctcactta tatagttaaa
     6241 tctgttgaga aaataatcat tattatattt gttcaggata ctctggcaga tcagctttta
     6301 gtttttctac ttgttcattg atttgtgtga tacgtggttc ggcttggaat ttaaaatctt
     6361 cgatatcttt tttgattgct tcttggaaag caggaatcac agattcagct gtttcttttg
     6421 tttcgatcaa tgcgctttta aaatgattca ggctgttatt taaatcattt gtcagagttg
     6481 ttgcttcttc caattcttcg atcagtttct ttcttgtttc attcccgctc ctgggcgcca
     6541 ataacagacc tccgacagtg ccagcagcag ctccaaataa cagtcctttg aaaaagtttt
     6601 taatcatcgc aggacacctg ctttacgatt gtttcttgaa tagcttttag ttcttccgat
     6661 ccataactat cttgatgatt gacaaagtga atagaaaaat catcctcttt gctgtagcga
     6721 gggactaaat ggatatgtga atgaaaaaca gattgatagg ctagttcacg gttgttattc
     6781 aagatattaa gacctttcat ttctggaaaa gctttttcca atgcttgagc aatcttaggc
     6841 acacgcgaaa aaacctctcc tgcaagttcg ggatcatatt caaaaatatc tgctacatga
     6901 cgtttaggca caactaatgt gtgtcctttc gttgcttgag agatgtctaa aaaagcatag
     6961 accttgtcgt cttcatatat tttatagctt gggatttctt gattgatgat tttgcaaaaa
     7021 atacagtttt ccatataaaa catcctttct ataaagaata aatacttcag cttaacttta
     7081 ccataagtta aagctattgt aaggaaaaaa acaaaatacc gtcattttct tcgtggtata
     7141 ataaataaaa ataaatggcg gaggaaaaag aatgagttta attatcgatc atgtcacagg
     7201 ggggtacgga catctccctg tgttaaaaga tatcagtttc gatgtacagc ctggagagat
     7261 agtagggttg attggactga acggagcagg gaaaagtacg accatcaagc acattatcgg
     7321 tctgctggaa gcttcaaaag gaacgataga aatcaatcat ctatccttaa agaaaaatac
     7381 ggaagaatat cgcaaacaaa ttgggtatat tccagagact ccttctttat atgaagaatt
     7441 gacattgaga gaacatatcg aagtcactgc gatggcctac gatataccta tggatatagc
     7501 aatgaaaaga gcagaaaagt tgttggagac ttttcggtta tctaatcgtc ttgactggtt
     7561 cccagctaat ttttcaaagg gaatgaaaca gaaagtgatg atcttatgcg cctttttgat
     7621 cgagccttct ttatatatca tcgacgaacc atttttaggc ttggatccac tagctatcaa
     7681 tgcattgctt gaattgatgg tagaaatgaa aaaagaagga gccggtattt tgatgtctac
     7741 gcatatttta gctactgcag aaaaatattg tgatcgtttt gtggtcttac ataacggaga
     7801 gatacgagca gacggttctc ttgagatgtt gagaaaagaa tttcagctgc cggattcttc
     7861 tttggacgaa atctatattg ctttaacaaa ggagcaggca catgagtagt ttttttcaaa
     7921 aacgtttagc aagacatcaa aagaaaatga tgaaatatat gcgttatgta ttgaacgatc
     7981 attttgtttt agtttgtctt ttcttgattg gcggtgtggg attatattat tctaattggt
     8041 tgaaaaccct gcagccaccg tttgcaattg gagggctcat aatctctgta ttttggatgc
     8101 tctgtttatt cgtgggtaag atggctactt ttgcagaggc agcagatatt gtttttttac
     8161 ttcctaaaga aaaagaaatg caaacctatt tggaacgagc ttttcgctat tcttgcattt
     8221 ttccgttgat ctttttgata ttggcatgcg gattggccat gccattagtg gtggttagta
     8281 cggagcaatc cttttcgatg tttttcgtct atctgatcat attatggagt ttgaaattca
     8341 gtcatctgca aatcaagaga gcagaattgt ttcgactaga tcaacaagtt attcgttatt
     8401 ggaaattagg ctggtttttt tcttctctga tctttttgtt tctatcattt tattggctaa
     8461 tatgggcagg aatagcaggt gcattagtac aggcaagtct atattactgg tttttgtgga
     8521 agcaacaaga aaaaggattg gattgggaga aaatggtgca tgtagaagaa cataggttgc
     8581 atcagatcta tcaatttatc aatttattca cagatgttcc tgaaattacg gcaaagatca
     8641 aaagaagacg ctatttggat cctatattga ctcatattaa aagagaatca aaaaacactt
     8701 atctttactt gtttgcaaga agattggcaa gaggttctga ttttagtggt ctttatttcc
     8761 gattagtaat tataggtggg ttattgattg ccggagtagt cgacttttat tttagtctga
     8821 ttatcggttt cttattttta tatttgatcg gtttccaact gttaccttta gcgaaccagt
     8881 tccgctatat ggttctcgtt cagctttatc caatccataa gaatcagaag aagcaggcga
     8941 ttcagtatct actcacatgg gtattaatta tcgcgtcggt tatttttggt ttgattgctg
     9001 cagtcgtatt agatgggcag cagcgatggg ttccgctagc ggcttatttg attgttacta
     9061 tgctctttgt taaactatat gtaccaactc gtttgaagaa gatgtcagac tgaaaacaaa
     9121 gctgtgtagg gtaaaaatga gaatgaaaaa tagagcgttg acagtaagaa tgctgttcgt
     9181 tctatttttt ttgagaaaag ctggtttttt gaatggcaat taaatgaaaa agaacgtata
     9241 tgtgcttgac gtgtagtgtt caggcgacta gaataataag tactgcaaaa caaagagtaa
     9301 gaggacggga gaatgcatga tggagtttca gttagaccaa gagtggcggt tacgtcccat
     9361 caaaggcgac acaggaaaaa catacatagg catgcgtgat gaggacaaag tctttattaa
     9421 acgaaacacg actccgatgt tggctgctct ttcaagggaa ggaattacac caaaacttgt
     9481 ttggacaaaa cgaacgggga atggcgatac tttgacagca caagagtggc tggaaggtcg
     9541 cttgctgact cctaatgaga ttggtcaaag aaacgacgtg attgatgttt tgtaccattt
     9601 acatcattca aattctctta aaaccatgct gacccgtatc ggtggtcgtg tcatgacgcc
     9661 ggagaagatg ctgcaaaaat atgaaaaaga attaccggaa gaactccaga aaaatcaatt
     9721 tctgcaaatt gtccatcgtt ttcttcgtga gcagatcccg tcaatagata aggaaaactt
     9781 atcagtagta cacggagatg taaaccatcg taattggtta gtatgtaaaa attatttata
     9841 tctagtcgat tgggattcaa tcatgtttgc agaccctgcg gtggatattg caacgatttt
     9901 aggaaattat gtgccactgt ctagctggaa tcagtggctg atcagttatg gcatacgccc
     9961 gactgacgaa atgcttgaaa aatttcattg gtatgcggcc atgaatttgc tccaagaaat
    10021 caccagatat tgcaaacgtg aagatcatcg acggatgaac gaagaaattt tacaattaaa
    10081 acgaattttc agtggctgag gtttggccca aactctgcgg agatttggcc agaccttttt
    10141 aattagagga aaggaatgga gataaataat gcgtgtccga aatcgtccgg gagcagcgga
    10201 aatgttagcg gctcacccga attttgtaat tagtgatcca acattgtgga agggaaaatg
    10261 gaatgaattg tttgaaaacg atcatccaat ccacattgag attggtatgg ggaaaggcca
    10321 gttcatcact ggaatggcaa aagcccatcc tgaaatcaat tatattggag tagaaatgca
    10381 ggtcagtgtt gtttcgattg cactagacaa gttgatcgaa caaccgcttc ctaacctgaa
    10441 acttctacat gtggatggat cagcgttgac agagtatttc gctgacagcg aagtagatca
    10501 aatctatctg aatttttccg atccatggcc aaaaaaacgt catgaaaaac ggcgattgac
    10561 atataagact tttcttgctg tagatgaaca gattcttcgt ccaaatggag aaatccattt
    10621 caaaacagat aatcaaggat tgtttgagta ttctctggca agtttttctc aatatggcat
    10681 gatcctcaaa caggtatggt tagatttaca tcaaagtcaa tttgaaggca atattatgac
    10741 ggagtatgaa gaaaaatttt cttcaaaagg tcaacgtatt tatcgagtag aagctcaatt
    10801 tcaagataag accaaaaaga aaagtctgtg aaactaactt tctgctttca taaaaacatt
    10861 aatatccata aataaatctc agaaaatgaa cattacagat gtaatgtatt ttctgggatt
    10921 tatttgattt aaaatgtatt tatttcatcg gtttttccac aaggtggaat caattggaag
    10981 cgagaaaatg aaaataatcc tttgtcggtg atcttcaatt cgggtatgac agataatgtt
    11041 aagaaagaaa gtgtcaaaaa aggatcaaag gttttagctg ctcctagttg atgtgcttta
    11101 gttattaatt cattcaactg ttcatagacg atcgaaaaag gttcctgtgt catgattccg
    11161 cctataggaa gagaaacaca agctatttcc tgctgtttgt tgacaacggt catcccgcca
    11221 cctttttgaa tcaaaagatt agcagcatac agcatatcct cgtcgtttgt cccaacgaca
    11281 actagattat gcgagtcatg ggcaatcgtt gtcgctatcg ctccttgttt cagctgaaat
    11341 cctttcacta tgcctaatcc aatgtttccc gtttgatggt gacgctcgat caccgcaatc
    11401 tttaattggt ctttttcgat tgaaggaatg aatcgttcgc cttgtcggtc cactttttct
    11461 accaaatctt tagtcactag actattcggg ataatttcga tcacatgggc ttgtgaagaa
    11521 gaaagaagca gctctaacga atgtggattt aatggttgag cgtttagttt tggcagacaa
    11581 cacgtatatg aatttgaatg atcagcaaat aaatggtctt ggagttgccc agattgaaca
    11641 actaatgttc ccttatagaa aacttcatga attggcagct tggtaagatc gtctgttaag
    11701 aagaaatcag cttgataccc aggggcaatc gcacctagtg ttttcaaatc aaaacattct
    11761 gctgcattca atgttgccat ctgaatggct tgtaatggat cgattccaag tgcaatagct
    11821 tgacggacga tatgatcgat tgatccttct tttactaaat cattgatcag tttatcatcg
    11881 gtgacaaaga agcatcttct tgaattttca ggtgtgatcg ccggtagtag cgatgccaga
    11941 tcttttgcaa cggtcccttc tcgaaccatt aaatacatac caagatccaa acgttcttta
    12001 gcttcttttg cagttgtagc ttcatgatcc gtccgaatac ctgctgcagg ataaacattt
    12061 aactcttcca tgccgatacc agctgcatgc ccgtcaattt ttttcttatg ctggtgcatc
    12121 aattggatct tttctaggat gtctgtttca ttatttgcta ctgcttgata gttcatgact
    12181 tcagctaagc ctaaaacttc gggtttttgt aaaaaagggg ctaatttttt tgcatttaaa
    12241 tttgcaccat tggtctcaaa aggggttaca ggtacacaag aaggtagcat aaaaaagata
    12301 ttcatgagtg tttgtcttgc atcttctatc ataaactcaa gacctgtact cccagcaatg
    12361 tttcctattt catggggatc tgtcactaca gtagtaactc catgaagcaa ggatactttt
    12421 gaaaattcgc ttggtgataa aagtgagctt tcgatatgca catggccatc gatgaatcct
    12481 ggcactagat attgcccatt agcattatgt acgctttttc cttcgtattc accgattcct
    12541 acgatcattc cttcgcagat tgctacatct ttttccatga tttctttcgt aaagacgttg
    12601 acgatcttag catgcttgat cactaggtca gcaggctttt ttttacttgc tacttcgata
    12661 tatttcctta atgttgtttc caatggagtg cctcccaaga taaattttta taagtataac
    12721 ataaattcgg cttttgccaa tatttttttg taaattcatt ttatttgaaa caaaaagatc
    12781 aaaaagacgt atgttttttg tggaggggag aataatggaa ataaaacgat tatcggatca
    12841 aatgataagt gaaagttttg ccaaacatgc ccaagacgta tatatattgc cagcttcaga
    12901 agtattttac atctattttc gctatggaaa aaaacgaaca ttctggaaga aagtctcttc
    12961 ggcagatgga cagcaactga tctcaagatt taagtatttg ggacagatgg atatcggaga
    13021 aaaaagaaaa gctcagttag gtgcatttac ttattcttta gaaaaacagg aagttcgtct
    13081 gcggttgtct tctgtaggag attaccttca acgagagagt ttagtgattc ggctattata
    13141 cggtactttt gaaacacccc atcgttgctt tagttctcga gattttgcaa ctattttaca
    13201 gaaaaccgtc agacgtggat tatacctttt cagcgggaca gtcggatcag gcaagacttc
    13261 tctaatgtat cggttggctt tggaagaaaa attgcaagtg attactatcg aagatcctgt
    13321 agagatagaa gaaccgtttt ttctgcagct acagatcaat ccgaaaatag cacagactta
    13381 tgatacactt ttgaaactcg cattacggca tcgtccagac ttgctaatca ttggcgagat
    13441 aagagatgaa tcaactgcac atgctgcggt aagagcagca ctaacagggc accgggtttt
    13501 tgcaaccgta catgcacgca atcttgaagg tacattggtc agaatccatg aactgacaaa
    13561 gaaaaaagaa gagctatctg aatgtttagc tggtgtcatt tatcaagaac ttttggaaga
    13621 tgccaatcac catccttctg ttttatggag ttatcaattt atggaggaag aatcagtaaa
    13681 aaaatcatgg gaggaaagtt atgaagaagc tcaggaaaat atttggcagt cagaactatt
    13741 ctaaaggatt aacaagaagc cagcaaaacc agtttgcaca aaccatggga gaactgcttt
    13801 tatctggttt tagtctgcaa gaatcccttt atttattact cacgatccgt tcgtttccac
    13861 ctgttatttt ggcaaatatc caacatcagc taaaggaagg gcaagcgttt agtgaggtac
    13921 tttcgcaaat aggatatgga gcagagcaac tggtacagat cgagttagct gaacgtcatg
    13981 ggaatctgcc gacaactttg ttggaaattg ccaaacagtt tcggcttgtt gaaggatttc
    14041 gcaatgattt gaaaaagctg cttgcatatc cgatgctatt gctcaccttt ttgctaggta
    14101 tcttgtttag tatgcggttg ttcgttttgc ctcagcttat tggttcagga atggtcatgc
    14161 aagagcattg gggtattcag ctgatacaag tctttcattg gtatgttcta ctattcatcc
    14221 tagcggttac tctattggct ttactagtaa ggtatcgtct gatgaagttg tctcctgttg
    14281 atcagagtga atggataagc agacaattat tgattggacc gatatattcc ttgtatcaat
    14341 cggcttattt gtcactagag attggaaagc tttttcatga aggtttcgaa ttaaggcaaa
    14401 tcatagtctg cttaaaggaa acgaaacaag aaagtctgat tcagcatctt gcctctaagt
    14461 tgattcaagg attggaggca ggtatgtcat tggctgagca gttccagaat tatcgttttc
    14521 tatcagaaga attcagtaaa attgttttac agggagaggc aaagggaagt ttaggtaaag
    14581 aactgatttt ttacagcgaa tggacaagaa aacagctttt caaacgtctt catcactgga
    14641 tgcattttat tcagccaatc atttttttgg gcgtagcggc tttgatttta ttagtttatg
    14701 ccgctgtttt attacctgtt tacggaagca tagaggaggt tttgccatga aaaagagaaa
    14761 aatatcttat tatagcggtt ttacattgat cgaaatgcta atcgtcttac tgatcatcag
    14821 tgtattagtg ttgttgttcg tccctaattt gtcgcgttat aggaatcatg tagaccaaga
    14881 aagtcgtgaa gcgatcatcc agttggtaga cacacaaaaa gagctctatg cgcttcaaaa
    14941 taatggaaga gttcctactg tggaggaatt gttgaatgaa ggctatatca aacgagaaca
    15001 tgcagaaatc tatcagcggc cttgaaggta aggttttagc atcaggatat acgttgtttg
    15061 aatcactctt actgatttta ttacttacaa cgatcctttc ttttccagtt ctcgcatttt
    15121 ctgcatggaa gaatgaattg gcagtctatc aatttttcgg ccaatttgaa aaacgaatct
    15181 attctacgca aaaaatcgct attgtgaatc agatccaaac aggcttttat tggaatagtg
    15241 aagagaatca aatcattttt catgttccga atccagataa aataaattgg gaagtattag
    15301 atattcctga tgagattacc ttgaaacgcc atgcttctat tacttttgct gctagaaccg
    15361 ggaatgaaag ttcactaaaa gcttatcaat tttattggga agaaaaagag cggacgatta
    15421 cgtatcaatt ccagataggg agtggtcgct atacaaaaag aattgaataa aacaggattt
    15481 gttttgttgg agtcccttac agcgcttatg atcgcttctg tctgtgtcta cttagtgagc
    15541 tttggccagc tgtttttgct tcaggcagaa aagaaaagtg gggaggaact gctatttatt
    15601 cgaatgattt atgaagatat caagacgcag cggatgtatc acaaaagacc agagcgaagg
    15661 gaagaaatga tcccatacga tatccaaatt gtcgataata atggagtaat cgaaaaagtg
    15721 tttgtcaata tggagaaaga agcgatcagt attgaaaaaa caagtaattc cagcatttac
    15781 gctgattgaa tgtctaattg gactgatgat attgagcagt ctattgctca cattcaatct
    15841 tttgattcag caaaccatcc aagtaagtga atttttgcag cgtaaagatc aaaaagagtg
    15901 gctaatattt ttagcacaat tagaagaaga actcaagagc agtcacaatg tctctgtgaa
    15961 aaatcagcaa ctgcactaca cgatcgagga taaacagtat atcatcgaaa gatatcaggc
    16021 aatgatacgt aaaagaagaa cttccggcgg acatcaacct atgttaacaa gtatcactga
    16081 gcttcaatta ttggaaaaag aacacacaat ttctttttat gtacactttg aaaatggaga
    16141 agatggatat gccatatgga caaaaaacga tcagtagaaa atgttataag ggctctattt
    16201 tattttctgt cttgctttta tttcttttat gtacgttttt attgatggca gcattagaaa
    16261 gctaccgttt gtcaatggat cttaatatac gaatcaaaaa ttattatata gcaaaaataa
    16321 tgaagcagat ggctttatcg gaacagtcta cgcttgccga aagttcggaa ggagtttttt
    16381 actacacaac tggatctgta acatatcagt gggtacaaca gtccctattc ttagaggtgg
    16441 aagtctcacc gtttatcttt cggttcacag aagaagtaaa aaatgataca gatactagta
    16501 cagaatgatg gaatgttcgt gaaaacgccc tcttttctgt tataacactg tttttaataa
    16561 aaaaatgttt gccgtttcac aaataaaaaa cagtgaaaac gattgaaatt ttgtcaattg
    16621 ttcgatagaa ttaaacaggt gaaaaaattg ttataatata tctagaaatg aggtgcacca
    16681 tgttaccaga aaaaatggaa caagggttta atcaaaacct ggaagctatt cagcttttac
    16741 agaatgcatt gggaacttcc tttttagaag cctatgtcga aaatgcagaa aatttaatag
    16801 atgattatca agtacgtgtg gtggatggag tgccgacaaa agagacaacg caacggatca
    16861 cagctcttta tgaagagttg aagaaacttt ctttcgagcc agaagaatgg cgcagactgt
    16921 ctcaattact tttattgaag ggcagccaga cagaacattt gcaggcgaac catcaactca
    16981 ctcctgatag tatcggtttt ttatttgttt ttcttattga acaactatac acaaataaaa
    17041 aagcaccggt taagattctg gatattgcgg caggaatggg caacttgtta ttaacagttc
    17101 ttttaaacct atcgaatgcg gggtaccaaa cagaaggaat tggtgtggat attgatgata
    17161 cattgttggc tgtcgcagct tctacaagtg atttgacaca agcaaatgtt caatactttc
    17221 atcaagacgg gttgcaggaa ttattaatag atcctgtcga ttttgcaatc agcgatttgc
    17281 caatcggtta ttatccgaat gacgaaaaag caaaagagtt tttaacaagt accgaagaag
    17341 gccatagtta tgcacatcat ttattgctag agcaatcaat gaaatatgtg aagccggatg
    17401 gttttggttt gtttttgatg ccgagtggat ttttagaaac ggatcaaagt gaagaaatca
    17461 aaaaatggtt taaagaagaa ggttatcttc aaggaatgat tcaattgcca gatgaattgt
    17521 tccgtaataa acaatcgcaa aaaagtattt tgatcttaca gaaaaaagga ccacaagcac
    17581 aacaagtaaa agaagtcttg cttgtgaaac ttgcttcatt aaaagaacca gagaaggtga
    17641 cggaattttt caacgagttt aaaaactgga aatcatctgc tctgtaatca ttcaacgaga
    17701 aggagagtca tatgtctaaa acaattgcaa tcaatgctgg gagttcaagt ttgaaatggc
    17761 aattatatca aatgccagaa gaaaccgtag ttgcgaaagg gatcgtcgaa cgtatcggtt
    17821 tacaagattc tatttttacc atcaagtacg gcgataatca aaaattcgaa caaatcatgg
    17881 atatcgaaaa ccacgaagta gcagtaaaaa tgctgttaga tcagttgatt gaactgcaaa
    17941 ttttaagctc ttatgatgaa atcaccggag tagggcaccg tgttgttcac ggaggagaaa
    18001 cttatgggga ttcagtagtg atcgatgaga aagtaatgga ccgcattgct gaattagcag
    18061 aatttgctcc tttgcataat cctgcaaact taatggggat caaagcgttt aaaaagattt
    18121 tacctgacat tttgagtgtg gctgtattcg atacatcttt ccactcaaca atgccaaagc
    18181 aaaattattt atatagctta ccggtgtctt attatgaaga tttcaaagta agaaaatatg
    18241 gcttccatgg cacaagtcat cgttatgtgt cggaacgtgc ggcaaaatta ttgaacaaac
    18301 cgatcgaaga actaaaaatc atcacttgcc acttaggaaa tggtgtatcg atcacagcag
    18361 tcgatggagg taaatccatg gatacttcta tgggattcac accattagct ggggtgacaa
    18421 tgggtacacg ttcaggtgat atcgatccag ctgttctccc atatttaatg gaaaaattag
    18481 agattgatat tgatgaaatg atcaatgttt tgaacaagaa atctggtctg ctaggcctaa
    18541 caggattgtc tagtgatatg cgtgatcttg aaaagaacta tgaaaaagaa catattcgtt
    18601 tagcttatga cattttcgtg gaccgcatcc gaaaatacat cggcggttat gtgacaacga
    18661 tgaatggtgt tgatgcaatt gtctttactg ctggtatcgg cgagaatgac ggacatgtac
    18721 gtagtgaaat catcaaaggt atgtcttggt ttggttgtga aatcgatcca gcgttgaatg
    18781 aattgcgtgg tgaagaagta gatatctcta caaaagaatc aaaagtaaga gtattagtga
    18841 tcccaacaga tgaggaacta atgattgccc gtgatgtgga gcgtctgcgc ggctaagcca
    18901 aagcattcgt taggataaaa gatgaaacgc atgcaagagg gaattcttgc atgtgttttt
    18961 tttgcggaga agaccgcaaa atggtaattt aacttcaatt tcccttcttt tttacaggat
    19021 cttcttaggg aattttgctt ggaaaagtgg tcaaaaggga aaagactggc attttattta
    19081 ttgattttca ggaaaatccc aatattttat tttcattcat ctttataagg gacattacat
    19141 atgtaaaaag aaaaaacatt tttagatcag cctcgtgggc tgtcgacttt attcttcgct
    19201 ttatgtttag ctagaaaacc gatccgaaac attcattgat cgaataataa aaacttgatt
    19261 ttccacctgt actttgcctg aatcgtgaaa gcacaggtat gaaaatcaag ttttttgttg
    19321 cgaaaaaacg tcctagtcaa tcagttggcc gagcggctgg cggctgatct gctcctgatc
    19381 aataacgagt gcttcttgga agtgttgctg caaaaagtag cggtcatggc tgactgctag
    19441 gagcgttcct tgatagtctt gcagcatttc ttcaatttct tctcgagcat agctgtcgag
    19501 gtgattggtt ggttcatcca aaatcaggac gttgattttt tctaaaaaga gtttcagcaa
    19561 cgccaaacgg attttttcac caccggataa atctttgatc cgtttaccca catcgctttg
    19621 ataaaagcca cttttggcta attgccgtcg agcgacttct tcgtttccag tcaatgtgat
    19681 ggcgtaagcc agcaaacggc tatcgggatc ggcaaactgc agctgctgcg gcaaataacc
    19741 gatttgcaag ctactaccta atttgatggt gccaccgtaa ggcttttcct caccgagtaa
    19801 taatttgagt aaggttgatt ttcctgcacc attgtcgccg atgatggcta aatgatctcc
    19861 acgatagact gcaaaactgc tgtctttgaa tagtacttga tcagcgtact gctggtgcaa
    19921 gttttgaatg atgaaaactt ctttgccaga ttttccacca ttagacaagc tttgcagccg
    19981 atttttcggg ggaggtggcg gtttgacgag tgtcagtttc gccaggcggc gttccagttc
    20041 tttggctttt ttgaagaagg actcattgtc tccttcatgt gcccactgac gaaaacgacg
    20101 aatcatgact ttcaaccgct ggacttcttt ttgctgcagt tcgtaatttt ttgtcagctc
    20161 agccaagcga gcttctttca agactcgaaa acggctgtag tttccgggat actcaatcaa
    20221 tgcaccttct tccagttccc atgtttttgt ggtgacttga tcgagaaatt gccgatcatg
    20281 agaaacgacc agaaccgatt tttttgtatt ctgtaaatag ttttccaacc agcgaatact
    20341 gttaagatct aaatgattgg tcggttcatc gagtaaaagt aaatcggcat cggctgctaa
    20401 aattttggct aaagcgactt taactcgttc gcctccgcta agttctgtta accgtgcttc
    20461 ctgacagtgt ggaatcccta agccttgcaa gatcgctgca atccggtctt ctaatacgta
    20521 accgcctagt tcttcaaagc gatgctgcag ctcaccgtaa cgggccagac acttctctaa
    20581 gtttgtatct ggttctgtca tcaatgcttc gtagcgactc atttgctgtt tgaggtcctg
    20641 tatctcagcg gcagaatgga gcaagtagtg gttgacggtc gcttcggaaa ctgtaagttc
    20701 ttgttcgacc gtcccgatct ttaagccttt tttgcgactg ataacaccgc tgtcgacacc
    20761 ttctctgccg gtcaagattt gtaaaaaggt tgatttgcca gttccgttga tatcgactaa
    20821 ggcaattttt tcaccagctt caatttgaca ggataaatcc tcaaataatg gattcccgcc
    20881 gattgttttt ttaatagaaa ttgcttgtat gatcatgatc tctctccttg agaccactca
    20941 aaaaggccat ggttatccct ggcctgtgtt atttaaactc agattatcat gagaaaaata
    21001 ccttgagatg gccattgatt ttacgtgaat tcaactgttt tggacaaact tgtcccgtca
    21061 acagcaactg taaaaccaat tgcatggcta aatgcgatat ggtatcgttg gtaattttca
    21121 ttacttgtta ttttagtcat gcgcatcata gtattttccc tccttcgtgt agttgtcttt
    21181 tccagtgtac ctgaaagtgt cagaaagttc aactagcgct gacatttact taatatttat
    21241 cgatgtgtaa ttctgtcaga gagctttttt tatctgactg agggtaatgt ctcgtggacc
    21301 acatcattgt attctgagct aaaccagcgt tcaggcgaaa tcatatggtg tttgataaaa
    21361 aactttgtct acatgacttc cttttgccat ttgcttaata ccgttgcatt aaacagctct
    21421 tctctttgga aatctcacca acatcgcgtt caggagtatc gttgtggttg caatggcaag
    21481 cggtagcctt tcatcaattc ttgaatgatt taccgaatgt agcacttgcc ttctcgcggt
    21541 acccgaaccc gaaataaaat tttagtttgg cgataaaatc ggtatccgcc atcgatactt
    21601 catatttttt cgtatcagat tcatcggaaa cttccacatt gacgaaccaa taagctttgg
    21661 catgcttagg tcgttttaaa ccacaccagg gcgactaatc gccctggtgt tcactttttt
    21721 tattttactt gaactcattg actgttttcc ctttatttta aacattataa agaccgatga
    21781 gtgcttgccg cgtcttttgt ttttatcacg gttttttgtg caaaaaaacc tgccgatagt
    21841 tggcaggtcg tctacgtgtt aagctttggt gtggtcttta gctggcaatt ggttttctaa
    21901 atcagttcgg acaacaagca catcgcaagc tgcgtttcta ataacatatt cagaaacaga
    21961 accaataaat aaacgttcta cagcattcaa tcctgtggct cctaacataa tcaaatctac
    22021 ttgattatct tctgggattt ctctggcaat aatttgtttt ggtgagccat attctacgac
    22081 agaggttaca ttattcaagc cagctttctt cgcattgctt tcataatctg ccaatgtttg
    22141 ctttgccatt tcagtagctt gttctgcaag cattccatca aaggatgaaa cggtttggaa
    22201 agaacgtgtg tcaatgacat gtgctaatag aagttctccg ttatttcgga tagctacatt
    22261 gaccgccttt ttaaaagcta attcagcttc atcagaacca tctacggcaa ccatgatttt
    22321 tttgtattgt tgtaacataa gcattcactc ctcaagtcaa tcgtattgta ctcttttatt
    22381 gtagaacact ttagggaaaa agagaattat ttactgcttt ggttcagcaa ttttttttgc
    22441 aaatcggata ctgaaggtgg cagaaacaat aattacgatc agtaaaaaga ccaaggcaat
    22501 aaagcgttga ttgaaaaaag ctgttaaaat acccattacg cctaaaacag catatgcagc
    22561 accgtaaaat tgtaggaatc cttgattttt atcatttttg ttgatcaata caaaaaagat
    22621 atcctgttta ttaaataaat aatagctaac gaataataat actaaagtgg cgataatcat
    22681 taaaatccta atcatgaaca aatcctccaa aataagcaac aaatagaaaa agcaatttgc
    22741 caagagcaaa ttgctttatt ctttaaaaga tggtaatctc cgttaatgcc gttgtttgtc
    22801 ctgtagtatt gatcccgcga attcaagcag gtgggtccca tggtcgtagc ttcgaactac
    22861 caaatgataa ggcatgttac cagcagtgac acaagacggg tcccaagata tcaataaaaa
    22921 tgttcggtca acacacaaag aggacaattc gaacatcaca gatttcccac ctctatagta
    22981 gcatatgaat ttaaaaagag caatgagaga gctcgcagaa acagaaccgt tttcttgtct
    23041 agttttttgg aggatgcttt ttccactctt ttatacggta atactgttga cccagagcct
    23101 gttcatattt ccctgtatct tttggctgat aatactgagc attcttcagt ttatctggta
    23161 aatattgctg attgacccaa gcctgatcaa aatgatgagg atactgataa cccacgccac
    23221 ggttgagtga tttcgcacct ttgtaatggc tatctctcaa atgatcagga acatctccag
    23281 ctttgccctc tcgaatatca gcaagtgctg catctaatgc catgtaggca gaattggatt
    23341 ttggcgacaa gcaaagatcg acgacgacgt ctgctagtgg gatacgtgcc tcaggcaatc
    23401 ccaatttttc agcagctaat acagcgttca ctgtacgagc cgcagcagca ggattaccta
    23461 aacctatatc ttcataaccg ataaccatca gacgccggca aatacttgcc agatcacctg
    23521 cctcaactaa acgggcgaga taatgaagtg cagcatccac atcacttcca cgaatcgatt
    23581 tttgaaaagc cgaaataacg tcataatgag catctccatt tttgtcatgg gtaagagctt
    23641 ttctttggac acattcttca atgatcgaca gtgtaagatg aatccttcct tctttatctg
    23701 aaggagtcga tagtgtagcc agttccagcc cattcaatgc actccgcaaa tcgccgttcg
    23761 ttgcacgcga aaggtgaaga agagcatctt cgtctaactg gattgcttgt tgtccaaggc
    23821 cgcgttcttt atcccttaaa gcatgttcga ctgccaactg gatgtcttgt tctgttaatg
    23881 gtttgacttc aaagatctgt gtacggctgc ggatcgctgg attgatggta atataaggat
    23941 tctcagtagt cgctccgatc aagatgattc ggccactttc taaatgagga agcagaaaat
    24001 cctgttttgt tttatctaat cggtgtactt catccaataa gaggatcact gtaccactca
    24061 tttttgcttc ttccgctaca acttgtagat ccttttttga atctgtagcg gcgtttagca
    24121 tgcgaaatgc ataattagta gagccggcga tcgcacttgc gatactcgtt tttccagtac
    24181 caggaggtcc atatagaatc atggaagaga gcatgcgagc atcgaccatg cggcgtatga
    24241 tttttccttc cccgactaga tgctgctgac caacgacttc atcgatcgtt cgaggtctca
    24301 tgcgataagc caatggctgt tgcataggat tttcctcctt cttgttttta cgtcccttaa
    24361 ttatatcata tttagcaaga tgcctagaaa gaacagcctt ctgttgtttt gttttcatag
    24421 cttatccaac taatattgtg tatgataaag taggtgagaa aaatgacgga taaacaaaag
    24481 atagatgaat ttttttggaa taaaacgacg ccagaagtgg cacgggattt attggggatg
    24541 tatttggagc atgacacacc agaaggaaga ttagctggat atattgtaga cgcggaagca
    24601 tacctaggtc cagaagacga ggccgcccat agctatggtt taagaagaac accgcgtgtg
    24661 cgggcaatgt atgaaaaacc aggtacgatc tacctttaca cgatgcatac tcatcggatt
    24721 ttgaacatca ttactcagcc agaagggatt cctcaaggag taatgatacg tgcaatcgaa
    24781 cccgccgcaa tgattgatca aatgagtaaa aatcgtggcg gaaaaactgg tccagatatc
    24841 agcaacggtc cgggaaaatt agtggaagca ttagcaattc ctcaagaact ttatggtcag
    24901 tctatagcag atagttcgtt acgcttagtt tttgagaaaa aaaagacacc aaaaaagatc
    24961 aatgctttgc ctagaatcgg tatcccaaac aaaggtgtat ggacagaaaa gccgttgcgt
    25021 tttgttgtgt cgggaaatcc ttatatctct ttacaaagaa aaaaccaaat cgagaaagat
    25081 tggggttgga ggaaagaaaa tgaaaaagaa ggatctatta acatatttag atgaaataat
    25141 tgaaaaacaa gtgacagact atgatgtagc tttagattgg gatacaaaaa atcatacgat
    25201 cgagatcgtt gttcgcttgt ttgcagaaaa taaagcacaa tttgagattg atgatgcaga
    25261 agggatcgtt tcgcaagaac caattatcga atttgaagac ggcgtacttc tttttaatcc
    25321 tcagaaatct gtttttgatg aacgcaatta tctagcagta atcccttatg aaggaaaaaa
    25381 agggttggca aaatcagtag ctgattcttt agtggaatat ctaaatgaag tattagctca
    25441 agggcaaagc gatttgcttg attttttgga tgaagacgat gacgaagctg tattcgagct
    25501 tcactgggat aatcaacaac tagcagaatt agtagaagta aaacagcaaa atgaagcaga
    25561 tacttattta ccgtacccaa gttattaagg agtgacagat atgaaatgga atgaagtaaa
    25621 agtagaaact gaaagtgaag cagtcgaagc agttgctaat attttgatgg aggcaggggc
    25681 gagtggtgtc gcgattgaag attctttaga tgttgaacat tttcagtcgg atcaatatgg
    25741 tgagatttta gataaaaatg attttacatc gctgaaagaa ggcgcactag tcatggctta
    25801 ttttccggaa acgactttct taccggaaat cttgcctttt atcaaagatc gtatcacaca
    25861 attacctgag tttggtctag ctattggaaa aaataaagta acagttagtg aagtagagga
    25921 aagcaactgg gcaacagcct ggaagaaata ctatcatccg gtgcgtatta cgcgaatgct
    25981 gacggttgcg ccaagctggg aaaactacca gacagaagat cctctagaaa aaattatcac
    26041 actagatcct ggtatggcat ttggcacagg cactcatccc acgacttcct tgacattgac
    26101 agcactagaa acagtcttgc gtggtggtga aacattgcta gatgtgggaa ctgggtcggg
    26161 cgtgctaagt attgcagcaa aatatcttgg tgcaaaagat gtctatgcct ttgatttaga
    26221 cgaagtagcc gttcgttcgg cgaaagaaaa tatggatatg aatgaaatag ccaaagatgt
    26281 ccatgtatca gctaacgatc ttttgacagg aatcgagatt gaaagtgatg taatcgttgc
    26341 gaacatttta gcagatatca tcttattgat gatcccagat gcttggcgtt tactgaagca
    26401 aacaggcacg ctgatcgttt caggaatcat tgaagagaag aagcaaatgg ttctggatgc
    26461 catgttagaa caagggttta tcgtggatca aattttccag caaaaagact ggtatgcaat
    26521 tattttgaaa aaaccagagg tggaatagat gcagcggtat tttttaaaag aatcatatga
    26581 atcaaaagac tattttgttt tagaagggga aaactatcac cacattgtac gggttatgag
    26641 gatggaaccc gaggatgaag tttttttggc attcaatgat caaatagcca ttgttgcaga
    26701 aatcacggag attactgaag aggctgttta tctgaaagaa attaaaaaag aagaaaatca
    26761 aaaggaactg ccaagttcga ttaccatagc gagtggctat cctaaagggg aaaagctgga
    26821 ttggatcgtt cagaaatcca ctgaattagg agcgcaagct tttcttggtt tcccggcatc
    26881 agcctctgtc gttaaatggg atgcaaaaaa acgtatgaaa cgacaacaac ggcttgaaaa
    26941 gattgctcaa gaggcagcgg aacaatccca tcggcagatc gtgccatcag tagagttatt
    27001 ggaaacgaaa caagatttat ttgagcggct agatgattat gacgtggtct tggttgctta
    27061 tgaggaatca gcaaagcaag gcgaaaaatc aaatgcagcc aaaattctta gtgaaacaaa
    27121 accagatagt cgaatcttag ctgtctttgg accagaaggt ggattatccc gcgatgaagt
    27181 cgattatttt gtagaacaca aagggatact ttgtggatta ggaccaagaa tccttcgtac
    27241 agaaacagca cctctttatt tattaagtgc tgctagctat caatgggaat taatgaatcc
    27301 ttctttcagc taacgttctt cattaataaa agagtatcga cagaaagaat tgaaaaaata
    27361 gtcaacaatt tgtataatag aaacaattat ttatacaatg gatacgaaaa atcgtttgaa
    27421 aaagctttta aaaagcgaac atttccgtaa aaaaacgtat aactgcacgg attttgggac
    27481 aattatcaaa cagatatttg tcccgattcc cgttttaccg aaaaaagaaa gggggatcga
    27541 ttcctgtgac aaaagaagaa attttaacgg gacccggtgt cataaagctc gttagtcaat
    27601 acatgggtcc cgaacatgta gcatttgttg agaaagcatg cgaatacgcg actgctgcac
    27661 atgacggaca gtttagaaaa tcaggcgaac cctacattat tcatcctatc caagtcgcag
    27721 gtatattagc agatttaaaa atggatcccc atacagtggc tacaggcttc ttacatgatg
    27781 ttgttgaaga tacagaaatc actttagaag atctgagaga agaatttggc gatgacgttg
    27841 ctatgttagt ggacggcgta accaaattag ggaaaataaa atataaatcc cacgaagagc
    27901 agctagcaga aaaccaccga aagatgctgc tagcaatggc tcaagattta cgagttatca
    27961 tggtcaaatt agctgataga ctacataaca tgcgtacgtt gaagcacctg cgagaagata
    28021 agcaaagaag gatcgctcag gaaactttag aaatctatgc gcctcttgca catcgtctag
    28081 ggatcagccg gatcaaatgg gaattagaag atacagcact tcgttatcta aatccaaaac
    28141 agtattaccg tatcgtccac ttgatgcaga cgaagagaga agaacgtgaa aaatacgtaa
    28201 gtggtactgt tgaagatatt cgaatagcga cagaagagct ggggattttt gcagaaatct
    28261 atggacggcc aaaacacatt tattcaatct atcgtaaaat gaaagatcag aaaaagcagt
    28321 tcaacgaaat ttatgacttg ctagctattc gagtgatcgt agattcaatc aaggattgtt
    28381 atgctgtatt aggagcaatc cacacaaaat ggaaaccaat gcccggcaga tttaaggatt
    28441 atattgccat gccaaaagct aatatgtacc aatctttgca tactaccgtg atcggtccgg
    28501 caggaaatcc ggtagaaatt caaatccgaa cacaggaaat gcatgaaatc gctgaattcg
    28561 gggttgctgc acactgggcc tataaagaag gaaaaaatga aaaagtagaa ccagatggta
    28621 tgacgaaaca attaagctgg ttccatgaga tactcgaact tcaagacgaa agctatgatg
    28681 cttctgaatt tatggaaggc gtaaaaggag atatctttag tgataaagtc tacgtcttca
    28741 caccaaaagg agacgttact gagttaccaa aaggatccgg accattggac tttgcataca
    28801 gtatccatac agatatcggt aacaaaacca ctggtgcaaa agtaaatggc aaaatggtgc
    28861 agcttgatta caaattgaaa aacggagata tcattgagat catgacttct ccaaattcat
    28921 ttggcccaag tcgcgactgg ttgaaattag ttgctactag caaagcaaga aataagatca
    28981 aacgtttctt caaagcccaa gatcgagaag aaaatgtgat caaaggccac gaatccgtgg
    29041 tcaaatgtat tacagatcta ggatttacgc ctaaagatat tttgacgaag aacaaactgc
    29101 aagaagcact cgatcgtttt aattatcaaa cagaagatga tctctatgca gctgtagggt
    29161 atggagaagt tagccccttg acgatggcca atcgtctgac tgaaaaagaa cgtaaagaac
    29221 aaaaaatcga gcagcaaaag caagaagcag aagaaatcat gaatcagccg aaaaaagaac
    29281 ctgacaaaat gaaagtacgt catgaaggtg gcgttgtcat tcaaggggta gaaaacttac
    29341 taattcgtat cagtcgctgc tgtaatccga ttcctggtga tgatatcgtt ggttatatca
    29401 ctaaaggcag agggatatcc attcatcgtc gagattgtcc gaatgttcag cctgacaaac
    29461 caaatgtagc agaacgtttg attgaagtcg aatgggaaga tacatcgaat acacgaaaag
    29521 agtatgatgc agatttggaa atttacggct ataatcgttc aggcttattg aatgatgtac
    29581 ttcaaacagt caatgcgcta acgaaaaatc tcaacagcgt tgaagcacgg acgaataaag
    29641 ataaaatggc gacgatccat ttgacggttg gtatccagaa tttatcccac ctaaagagta
    29701 tcgtggataa aatcaaagca gtacctgatg tctacagtgt acgccggacg aatggatagg
    29761 aggacagata tgagagcagt tgttcaacgg gtaagtaaag ctgctgtgtc agtcgatcag
    29821 caagaaattg gaaaaattgg ctacggccta cttgttttat taggcattca tgaaaccgat
    29881 acgcaaaaag atgtggacta tctggtaaaa aaaattgcac aaatgcgtat ttttgaagat
    29941 gaacaaggaa aaatgaatat tagtgtagaa gatgtgggag gacaaatttt aagtatctct
    30001 caattcacac tgtttgcgga tacgaaaaaa ggaaatcgac caagctttgt ggaagccgca
    30061 cgaccagaaa cagctattcc tttatacgaa tcattcaatg aaggaatcag aagtcgcggc
    30121 atcacagtcg ctaccggaca attcggagca gatatgtcgg tttctctcgt caatgatgga
    30181 cctgtaacga tcattattga ttctaaaaac aaataaagta atctggtttt cactagtaaa
    30241 aactatgaaa cacgtcgctt aagttagact gataaactct aacttaagtg gcttttttgt
    30301 ctgcaaaaag cgcttctttt gtcagcagtc ggaaaaaaag ttaagcttaa attatacaag
    30361 ttatttttta taggaggttg ctgaagatga aagaagtacc agtagtagat atcaaaatca
    30421 cagatttaaa aaaattgtat caaaaaggat acaacatgtt ggaggagtta cgccatgaag
    30481 cggacgctcc cgtcgtcaaa gcaaagattt tcaataaaga agctatcacg atctacggat
    30541 catcagcagc aaaagtattt tatgatccaa ggaatttcaa acgaaaaggt gctatgccaa
    30601 aattagtatt gaagacactt tttggtcaag gtggagtaca gactttggat ggagctgctc
    30661 atcaccatcg gaaaaatatt tttatggatc taatgacacc ggaaagaatg gaagattatc
    30721 atcgtatctt agataagaat ctgacacaag cacttgaagc acagcatggt caatttgaac
    30781 tatttgattt aagtaagatg gttttcttta catctatttg cgagtgggca ggtatcaatt
    30841 tatcagcgat ttctaaagat gaggtcgaaa aattagcaga atatcaaatc tctatgatca
    30901 gcggaacgtt tacttctccg attgatcaca taaaaggtgt cgagaatcgc aaaaaatcag
    30961 aaaaatgggc gcaaggcttg attgaagaag ctcgacaaaa tcctgtggct ggaaaagaaa
    31021 atgtggcatt atatgctttt gccaatgcca cagatttaga tggtcagctt ttacctctgg
    31081 aagtggcagc tgttgaatta ttgaacatca tccgtccaac ggttgctcta actgtgtggg
    31141 cggcattgat gggacatgca cttttcagta gaccagattt gtatcagcaa ttaaaaaatg
    31201 acttttcgac gttacaagat ccgtttattc aagaaatgag acgctattat cctttcttcc
    31261 caatgctacc tgcgatttcg ctgaaagaag tagaagtaga tggttatcgt ataccagaag
    31321 gcagttgggt tattttagat ttatatggaa cagaccatga tgaacgaaca gttgaagcgc
    31381 cagattcctt tatgatcaaa cggtatgtag ggaaagcaaa agatatttcc tataaagaag
    31441 aatatgagat gattgcacaa ggcgggggaa attttcgaca aatgcatcgc tgtgcaggag
    31501 aatggatcac tcttcatagt ctacgtgtgt tttccgatca gctagtaaat aagtttgagt
    31561 tctctgtacc tgaacaagat tggacgattc cattcaatca gtttccgact tatccgaata
    31621 gccgagcgct actttacaag aattagatta gcataaactt atcgaaaaaa aggaagatca
    31681 aacagtgctg atcttccttt tttcatatta ctataaattg ataaggtcaa ttgatcaaaa
    31741 tccacgaaat gcttcatcaa tggtttgata atcttcattg gataaatgca aatctaaagc
    31801 ttggacattg tcagatactt gctctggacg cttagctcca ggtataacta cagacactcg
    31861 tggattcttc atgtaccaag ctaaaacaag ttgcgcagga gtggcttggt atcttttagc
    31921 aatggggcga agtacatcga cagcggttaa aatttctctg aaacgttctc cttggaaatc
    31981 tggatttctt tttcgaggat ctccttcacc aaattgtttt tcctcaccaa gttcatattt
    32041 tcctgtcagc aaaccggaag caagaggaaa atagggaaca aaactgattt tgtttttttc
    32101 aagataaggg aataattctt tttcagcctg ccgatggatc aaactgtatt tatcttctac
    32161 tacatcgaca tatccatctg cattcgcttc tttcaattgt tctaacgtga agtttgacac
    32221 accgactgct cggatttttc ctgcttcttt taattcgtgc aatgtagcaa ctgattcatt
    32281 ttttggtgtg ctttcatccg gaaagtggat ataaaaaatg tcgatgtaat cagtttgcag
    32341 acgttttagt gcatcttcca ccgattgttt gagaaactca ggagaattat caaatgttcg
    32401 ccctttattt ggtacatgag cagcttttgt agcgatgacg acacgtgagc gatcatattc
    32461 tttttccttc agtacctcgc caatcagttc ttcggaacgg ccgtttccgt aagcataagc
    32521 ggtatcgatc aaattgatcc cgctattcaa agctgtacga actacttgtt ttcctgtctc
    32581 atcttccaaa ccgtcaaaaa gattatgtcc tccaacggca tttgctccta atccaagctg
    32641 ttctgcgtag acttgtgaat gtccgattct tatttctgcc atagctgatt cctccttttt
    32701 gtctagtcta aaacaaaata ccaaaaagct gaactaaaac gtttttgtga ggggaaatag
    32761 tcagatatct cttgactttg agactggttt ttagtaggtt ataagtaata tcgaatgtca
    32821 atgaaagagc gagtaagttt ttgaaacttt acaagagagg atcatcgagg ctgaaaatga
    32881 tctaaagttt cattagccta aagacactct ggagatggac gagtgaaaag ccgtcccgcg
    32941 tacgagcgtt attcgtaaga ggaaagagaa atctttcaaa attaggtggt accgtgtatg
    33001 aatttcattc acccttgttc atgatgaaca agggtgattt ttttatttac atagctaaga
    33061 aagggagaga aaactatgag tttccaaaga ccaaaaggaa caaatgatat tttgccaggc
    33121 gaatcagaaa aatggcagtt tgtcgaagaa acagcacgtt tattattcag agattatcag
    33181 tataatgaaa tgcgtacacc gatttttgaa cactatgaag tgatatcccg aagtgtaggg
    33241 gatacaacgg atatcgtttc aaaagagatg tatgattttt atgacaaagg ggaacgccat
    33301 gtgacgcttc gaccagaagg tacagcacca atcgttcgtt cttttgttga gcataaattg
    33361 ttcggcccag agtatgcgaa accgttcaaa actttttata tgggaccaat gtttcgctac
    33421 gaacgccctc aaaaaggacg gttacgtcaa ttccatcaaa tcggagtaga agcatttgga
    33481 tcagaaaacc ctgcaactga tgtggagagc atggtgatgg cacttgattt cttcaaacag
    33541 ttggggatca cgcaaatccg gctagtcatc aattcattag gtgataaaga aacgcgtcaa
    33601 gcctatcgtc aagcattgat cgactatctt ttaccttttg aatcagagct gagtgaagac
    33661 tctaaacgcc gccttcatga aaatccatta cgcgtattgg acagtaaaga caaacgagat
    33721 caaaaatttg tggcagaagc tccatctatc ttagattatc taagcgagcc tgctcaaaaa
    33781 cattttgata ccgttgtttc catgctggat gcattggctg ttccttatga aatcgacagt
    33841 aatatggttc gcggactaga ttattacaca catacgattt ttgaaatcat gagtgacgca
    33901 ccaaaaatgg gggcacaagc aacgatttgc gcaggcggac gttatgacaa tctagtggaa
    33961 gaactaggtg gtccttcaac acctggcttc ggatttgcaa tgggtatcga gcgattactc
    34021 ttgactatgg aggcagagga agtagttatc cctgcattta atgagttgga tgcttacgtg
    34081 gtcgcactcg gagacgaaac aaatatcgaa gcattgaaag tggtccaagc gattcgaaac
    34141 tttggttttt cagctgaccg cgacttcatg aaccgtaaag caaaagcgca atttaagaca
    34201 gcagataaag cgaatgccaa attagtattg gtgatcggtg aagatgagct tgctagtcaa
    34261 acgattaatg tcaaagcaat ggcaagccga aaagaaaaaa gttttccatt agaagagata
    34321 tatgaacatt ttgatgaagt ctatgatgaa atgactttat tcgagtagga ggaaaaaaat
    34381 aaatggcaca aagaacgatc tattgcggat tagtttccga acaacaatta gaacaaacag
    34441 ttacattaaa aggatgggta caaaaaagac gagaccttgg gggagtaatc tttatcgacc
    34501 tacgtgatcg tgaaggaatc gttcaaatcg tctttaatcc tgaaatcaac aaagaagcat
    34561 gggaaatcgc ggataaatgt cgcagtgaat acgtgatcga agtgactggg gaagttagaa
    34621 gacgcggaga aaatgcagta aatcctaaaa tggccactgg tgaattcgaa gtgattgcaa
    34681 gtgaaatcgt gattttgaat caagcaaaaa ccccgccatt tcaaattgaa gatgatcaag
    34741 caatcagtga cgaaatacgt ttgaaatatc gttatttgga tctgcgtcgt ccaaaaatga
    34801 ctaaaaacat caaattaaga aatgcgacaa caaaagcgat tcgtcattat ttagatgata
    34861 atgactttct cgatatcgaa acaccttacc tagggaaatc tacaccagaa ggcgcaagag
    34921 actacctagt tccttcacgt gtccatgctg gacatttcta tgcgttgccg caatctcctc
    34981 aattgtttaa acaattgctg atgaatgctg gattcgaccg ttattatcaa atcgttcgtt
    35041 gtttccgcga tgaagattta cgtggagacc gtcagcctga atttacgcaa gtagatattg
    35101 aaacaacgtt cttaacagca gaagaaatcc aaacttacac agagggattg attgcgaagg
    35161 taatgaaaga agttcgcggt atcgaagtca cattgccatt tcctcgtatg acctatgatg
    35221 aagcaatggc tcgctacggc agcgacaaac cggatacacg ttttgatatg gaattgatcg
    35281 atctaagcga tacagtcaaa gaagtagaat tcaaagtctt ccaaatggct cttgaaaacg
    35341 gcggtgtcgt gaaagcattg aatgctaaag gcgctgcaga tcgctactca cgaaaagaca
    35401 tggatcagtt aggtcaatat gtaggacaat tcggagctaa ggggcttgca tggctgaaag
    35461 tagaagaaga cggattgaaa ggtccaattg ctaaattcat gggagaagca acagaagcaa
    35521 tcattaaagc aacagatgca aaaccaggtg acttgctgat gtttggcgca gataaaagtg
    35581 aaatcgtcgc tgctgcatta ggtgctatcc ggacacgttt aggaaaagaa ttaggactaa
    35641 ttgatgaatc gaaattcaat ttcttatggg taactgattg gccacaattt gaattttcag
    35701 aagaagaagg tcgatatgtc tctgctcacc atccattcac gatgccaaaa gaagaagata
    35761 tcccgttatt ggctgaagac cctgcaaaag tctatgcaga agcttatgat atcgtcttga
    35821 acggttatga attaggcgga ggttctatcc gtattcatac tcgtgagctt caagaaaaag
    35881 tactagaaac tctaggcttt acaaaagaac aaatggaaga acaatttggt ttcttgttaa
    35941 cagctttaga ttacggattc cctccacatg gcggtattgc attaggattg gaccgttttg
    36001 caatgttatt agccggagaa gataatattc gtgaagtcat tgcctttcct aaaaacggaa
    36061 aagcagcaga tgtgatgagt gatgcaccaa gtatggtttc gcctttacaa ttgttcgaat
    36121 tgaatattga tgtaacgaat atagaagaat agccgataaa aaggtaaaaa ggatttgtcg
    36181 aatacgcttc ttttgacaac cttgatttaa ttgaaaaaca ctttataatg aagccataac
    36241 atataaagcg gggcttttcc atgaaaaaaa cattcgaaaa ttgggcttat catgattaca
    36301 cgaccaagct ttcggtatct atcgtttatg cgttgatcgc atcagtcgca ttgaatttct
    36361 tttatcagcc aggacatatt tattcaagtg gtattactgg tctagctcag attttgacga
    36421 ctttgtcaaa atcactgata ggattcgaag taccggtatc aacaactttg tatttactga
    36481 atataccttt gtttgtattg gcgtggttaa aaatcgggaa gaagtttaca attttcactt
    36541 ttatcacagt cactttgacc tccctttgta tccatatttt accggaaaca gtcctatcta
    36601 ccgatccaat catctgtgcg ttgtttgggg gggctgtgat gggactaggt attgggctag
    36661 cgttgagaaa cggcctatca tcgggcggtc tagatatcgt gagtatcacg attcgtaaaa
    36721 aaacaggaaa gtcgattggt tctatttcga tctacttcaa tgcattgatc gtttttgttg
    36781 ccggaattct atttggctgg caatatatgt tttacagtac gttgtctatt ttcgtcagtg
    36841 gaaaagtaac tgatgtcgtt tatactaaac aaaagaagat gcaggtaatg atcgtaacga
    36901 aacatccaga aaaagtagta aatgaaatcc aaaactgtat gcgtagaggg atcacgatca
    36961 tcaatgaagc agaaggtgct tataagcatg ataagcagac agtattgatg acgatcgtta
    37021 cacgatacga actgcctcta cttagagaag caatgaaagc ttctgatcca caagcttttg
    37081 taagtattgc agaaaacgtc cagatacttg gtcgtttcta tgaggatggc atttagatcg
    37141 agtttgacaa aaattcatga aatactgctg tagacagacg ttcgtttgtc tgcagttttt
    37201 ttctaccata gtagaatcag caaagaaaaa ctgctttttt atttgaaatt ttcttaaagt
    37261 tgaaaaaaag catgtttatt tttttaaatt tatgggatga tagaaaaaaa tagtagagaa
    37321 aggacacgat tctacttgct attttcactt aatacccgta tattacgaat gaaaaaagtt
    37381 tatgaaaagt tggaatattg aatggaaaaa caaaaaaggt taaaaaacag ccgctacatc
    37441 actgggtttg atgggctccg ttcattagct gtggtgggtg ttatcttcta tcaccttctg
    37501 ccaactagta tgaaaggcgg ctatttgggc gttccgattt ttttcgtcgt atctggttat
    37561 ttgatcacgg atctgctgcg gcaggaatgg gaacaaaatg gaaaaatcaa tatttggcaa
    37621 ttttatgtca gaagaatgaa gagactctat ccggcacttg tttttatgct tgtgacagct
    37681 tcggcttata tcactttgtt tcagcgaggg ctgttaaaca atcttcgagg agttgtggtc
    37741 agcagcttac tgtatgtcaa caactggtgg caaatcaata acggtttatc ttatttcgat
    37801 cgatttgcta atgagtcacc gtttacgcat atctggtctt tagcagtaga aggtcaaaac
    37861 tatttggttt ggccgatctt gtttgtattg ttgatggtat ttgttcgtaa aagaaaatgg
    37921 atcttttata ctgtgttagg tacatccttc ctctccgcca tcttgatgat gatcttgtat
    37981 acaccaggag gcgatccaac ccgtgtttat tacggtaccg acactcgtct gttttctata
    38041 tggttgggaa gtgcgcttgc gtttgtttgg ccaagtacgc gtttaaagaa aaacatcccg
    38101 aaacaagcga aaaatattct gaatatcgct ggaggaatct cgcttttggc actgattctt
    38161 tgcttcttct ttttagatga tcattacaat ttcatctatt acggaggaat gctcctagtg
    38221 agtctgttat gcgtagtgct agttgcagtg acagcacacc ccggagcaag tttgaatcga
    38281 tggctgacaa atccagtatt tacttggatt ggcaaacgaa gctatggcat ttatctgtat
    38341 cagtttccag ttatgatttt ttatgaagca aaggtgactg atattgccga tcatgttttg
    38401 ttgcatactt tagtggaaat cacattgatt ctaatcatca gtgaactttc ttatcgatat
    38461 gtggagcgtc cgctagcacg ttttgattat ggtcgtacat tccaagtgat gaaaggatgg
    38521 ttcagcaaac cgatcatcag taagagcaaa ccatgggttg ttccaggcac attacttgta
    38581 gcaattgcac tcgttggttt cgttacggca ccgaaaaacg ctgtaacagc cgatcaaaaa
    38641 caactgcagg caaaaatcgc tgaaagtaaa aaattagcag aagaaaccag aaacaattca
    38701 gaaaacacca ctagttcggt tgaccaagcg atccttgata agtacgagtt gtccaaggca
    38761 caagggaata aggcacaaaa attagaacta acggcatttg gtgattcagt tatgttggat
    38821 gctgcaacgg atctacaaga actttatcca aaagtagtgg tagatggaga tgtcggacga
    38881 caattatatg ccagtgtacc gtacattgaa aaattaaagg aacaagattt gttgaaagac
    38941 actgttctga ttggactagg aacgaatggc tcattctcgg aagctcagtt tgatagtgtg
    39001 atggatgcaa tcggtgatcg aaaagtatac tgggtcaatg tccgagtacc gacgaagcgc
    39061 tggcaaaatg acgtgaatgc gatgcttgaa cagatgacga aaaaatacga caatatgacg
    39121 ctgattgatt ggtacagcta cagtaatgac catagtgact ggttttatga tgatcaagtc
    39181 catccaaatc ctgaagggat gacacattac attcacttag tcagcgaagc cttgctagga
    39241 ggaagttcaa gtacgaacag cagtgagaca aacgtgacag aatgatagct acaaaaaact
    39301 tcaggcaaaa acatttgttt ttgcctgaag tttttttata tttaccgtgt ttttaacatt
    39361 catatagatt caggaagcaa aacggttgag tgtgtctgta aataacgttc tttttgtttt
    39421 cgggtgagtt ttttgaatgc agcttctgct tttgttgctt cgcttcttga agaaaatact
    39481 tctgcatgga tcatcttaac aggtcttctt ttttgaagac gagtatattt agcaccagta
    39541 ccattgttat gttcctctaa tcgtctagaa agatcggttg tatatccacc ataaaatgtc
    39601 tgatctttac agaataatac gtaaaaataa tgttcatttg ccatagagca tctcacgaat
    39661 ctctgcagtg tattctccat ccatattgta agtgatcaat ggtggagcga ctttgaatcc
    39721 gtctaatttg ccttctttga ttccttcaac taacaaaata tttgcctctt ttccaggttt
    39781 tgggtaaaca aattggatcc ttttaggtgc aattcgatag tcttccatga cctgaatgat
    39841 ttctaagaac cgatcaggac gatggaccat agtaaagcgt ccatttgttt ttagtagctt
    39901 acttgaaata tcgatcactt cttctaacgt cgtatgaatc tcatgacggg cgattgccag
    39961 gtgtttatta gggttcgttt tatttgttgg cagccctttg aaataaggtg gattacagac
    40021 gactaagtca caagaattat gtcgaacgac agataagctg tctttcagat cgatggtgtg
    40081 gacagtcatt tgttcattta aatggttaag cgcgatacta cgtcttgcca tgtctgctag
    40141 acgaggttga agttcgatag cgtcgatagc cgcctgggtt cgtttactta aaaaaagtcc
    40201 aactgcgcca tttcctgcac aaaggtcaac aatcttccct tttttaggta cacgggaaaa
    40261 gtgagcaagc agcacagcat ctaaagaaaa agaaaacact ttggaacttt ggataatctt
    40321 gatatcttct gcgtataatt ggtcgattcg ttcgtcttcg tgaagcatgg attccctcca
    40381 aaatgaaaaa aatagtcgtc tgtattatac tatttttatt aaaaagaacc aaggtggctt
    40441 gtcaaacctc tattttttat gcatactatg accataagaa gggaaaggaa gagactatgt
    40501 tttatcgatt tatgcgcggg gtagttcgag tactattagc cttagttaat ggaaatgccc
    40561 gctatgagaa taaagaagtt ttaccgcaag atgagaatta catactagtt gctccccatc
    40621 gtacttggtg ggacccatta tatctggcgg tagctgcagc acccaaggaa ttcagcttta
    40681 tggcaaaaga agaactgttc aaaaatccag ttctgcgatt tatattaact cgatccaatg
    40741 cttttccagt taaaagagaa aatccaggac caagcgtcat taaaacacct gtaaagattt
    40801 tgaaatcgac aaatttgagt ttgatcatgt ttcccagtgg gacaagacat gctacagaac
    40861 ttaaaggcgg aatggcattg atttccagaa tgtctaaagc gaaaatcgtg ccagctgtat
    40921 atcaaggtcc gttgacactt ggggatctgt tcaagcgtca gagagtgaca gtgcgtttcg
    40981 gcgaaccaat cgacttatca gatatcaaga aaacggataa agaaggtatg gcggagatcg
    41041 aacgacgaac acaagcagct tttgacaaat tggacaaaga agtgaaccct gatttcaaat
    41101 atgaagtcaa aaaaaaataa aatgataagt ttatgcgtgg gcggtctttt gaatctactg
    41161 tcctgttcag ttcagacttt ttcaatgaaa acgagggaga gacgtcaagt ctgccctctt
    41221 tttgcgttcc aatatgtaaa atagaagaca ggtgtcatgt aatggatcaa agtgttataa
    41281 taattagaag gacatttttt gaggtgattg aatgcgtttt ttacatacag ccgattggca
    41341 tatcggaaaa aaactacaag gttatgactt gattgaagac cagcgtcatg tactatcttc
    41401 tatactagat atcgcgaaag aagaaaaagt agaggcaatc gtgattgccg gagacttata
    41461 tgataggagt gtcccagctg ttgatgccgt agaattatac aatgaattga tggtcgattg
    41521 gaatttaaag gaaaagatcc cgatttttgc tatatcagga aatcacgata gcagtacgag
    41581 actttcagcg ggtacgccat ggttcagtta ttcgaactat tttttgcgaa cacagctagc
    41641 agaagcattt gaaccagtcg agtttaaaaa cgtacaattt ttctttcttc cttactttga
    41701 accaattgca gcgagattat attttgaaga tgacaatatc cgaacgatca agcaagcggt
    41761 agccaaggtg atagataaaa tgcaggagct atttgattca gaaaaaaaaa caagtattga
    41821 ttagccattt ttttgtaatg ggaagtgtaa aatctgattc ggaaacgaaa atcgaagtag
    41881 gcgggttgga ttcgatccct ggtgaactac ttacaccgtt tgattatgta gcactaggac
    41941 atttgcataa tcaagctgct ttgagacagg ataatgctcg ctatagcggt tccccattga
    42001 aattctcttt atctgagatc aaccaaaaaa aaggcgtatg gatcgttgac ctgacaacag
    42061 gcctggagtt gaattttagg gagatcgaac ccctgcatga tattgaagaa ataacagcga
    42121 gtttcaaaga attgctggat cctgcctttt atcagtccgt caatcgggag aattacatct
    42181 atatctatct cactgataga acgatcatcc cgaatatgat gaaccagctt cggcaaatat
    42241 atcctcgtat tttggggatt gaacgggcaa acggtcgagc agaacaaaca aagcgcaaac
    42301 gaaaagtact agaaatgaaa gatcctcaaa aattagcaga agcatttttt agtgaaatga
    42361 cggaagagca gctaactgca aaccaagaaa aatggctcgg agaaacgttg caagaaatca
    42421 tggaaatgga atgatcaaga atgaaaccga aaaaattaca acttaaaaat tttggtcctt
    42481 ttatcaatga gaccgtagat ttttcacgtt tgacagaagc accgcttttt ttgatcagcg
    42541 ggaagacagg agctggcaaa acgacgattt ttgatgggat caccttcgca ctttttggtg
    42601 aaacttccgg aagacttcgc tctggaaaag agatgcgttc tttgtttgct acaccagacg
    42661 aagaaacaag cgtgactttt tcttttgaac atcagcagat gagctatgaa atcgaacgaa
    42721 aaccagaaca agttttagca aaacgaaaag gaaacggaac aaaaaaacag gcagccaaag
    42781 taacgttgac catttttgat gggaaaggaa aagaaataaa acaaatcaca aaacgtacag
    42841 aagcagatca gttgatcaga gaactgatga atctcgatgc gaaacagttt tctcaaatcg
    42901 ttttactacc ccaaggagaa ttccgtaatt ttttggtttc atcaagcagt gataaagaga
    42961 ctgttttacg tcatttattc ggtactcaat ttttccaaca gttcaatgag cgattaaaag
    43021 aaaaagcaaa acaacagcaa caaaaattgg atcatttaga gcaagagctg catcttctgc
    43081 aaaaaaggtt tgttttagtc aaagaagagg aaccagcagc agggtttgaa gccgttttag
    43141 cccaatggac aaaccatcag gcaatactcg aaaaacaaat cacggaagaa aaagaagctc
    43201 taacggaact tcaggaaaaa caaaagcaac tagaaaattc ctactatcgt tttgaaagct
    43261 tgaaaaaaag ttatgaagaa aaaacgatat taataaacaa gcaaaaggaa ttagctgctc
    43321 aaaaagacga aatagaggaa aagaaacgat ggattgggta ttatgaattt acgcaacgat
    43381 tagtcgaacc aatccagcgc ctacaagaaa taaaagttga aaaaatagaa ctgtcacaaa
    43441 aaagtagtga gcaaacaaag aaactagcga tgactacgca gaattatgaa gagtggcaaa
    43501 aaaatgaagc aaaccgcgta cgccgacaaa caaaaatcaa agaagctgct caaaaactgc
    43561 aaaacaaaca gatgatggtg ccaattgtcg aatccttacg tagtaaaaaa caagacgcag
    43621 aacaattaac aatttcattg gataaagaag aattgaatgc cgagaagctg tatcaaaatc
    43681 tcgctatcta tcaggaaaga actcgaattc ttcaagaaaa tttactggat caggaaaaac
    43741 tgcaagaaga tcgtcttgct tttggacagt taaaatatct agatgaaaag cagcaagaaa
    43801 tagaaaaaca aaaagcaaaa gacactgggt cgctcgcaca gataagagaa gaactcaaaa
    43861 aagcaactga agaaataaca aaaaatagtc agctattgaa agaacagaca gaacaagcag
    43921 aacagctgaa aagccaatgg gcaaagcagc aaattgctcg tcttcagctt tttttgcgtc
    43981 caggggagcc atgtcttgtt tgtggatcaa cggaacaccc catacaacaa ttaaaacacg
    44041 aacagccaaa taaggaagat atcttaaaag tagaacaaca actggcagaa gctgagtctg
    44101 ctgtagaaag aactcagcaa atcattgcca aaagtgaagc acatgaaaat aatcttcaac
    44161 aacaattatc agatttagaa caaaaaatca tggcaacgaa tatacaattg gctgaacaag
    44221 aagaacactt gcaacaggct ttgaaaaagg catttcctga gataacagaa caaaaaacaa
    44281 aaacaactct tgtagtaatc gaacaatctt tagatagacg aaaaagcaag atggccaatg
    44341 aaagaataga gctggaagaa ataagacaac aagcagaaaa gctcgaacaa caattagctg
    44401 tctacaaaca gcagcagaca gaaaaaaata atcagctcca gcaactgcaa ggagaaatca
    44461 aaagtttaga acaacaattg caagatacgg atagtaactg tttagaagag gagatacgac
    44521 gacttgaaaa acagttagaa gaagactcga atcaactagc tgttgagcag aaagcaggag
    44581 tgcaattgtt gcaagatctt catacgcttc ggacacagct tgatcttcaa catgaacaaa
    44641 tagaaaaaat agatgaaaaa atagaaaagg aacaagaaag aatagctaaa aagttagaaa
    44701 gccagtcgta tttttcaaca attgaagaaa tctgtgcatt tgcacaacag caagcacaat
    44761 ataaagaaaa ccaacgtaca gttacttctt atcatgaaga gcaattagtg acagctgacc
    44821 ggctgcgtca attagaaaat atccagctaa ctgagacatt cccagataca gaagagatca
    44881 gccaacagtt agaagaaata agacgtatga tcgaaataaa gcaagaaaag atatacggtt
    44941 tacaagaaca aaaaattagt aatcaaaaaa tctacgatga atgtctttcg atttatcaca
    45001 caagccagac gaaattagat gagcttacgc aattacagca gctttcgcaa acattcaacg
    45061 gagaaaatcc taagaagact agtttggaac ggtatgtgct tcaggtttat cttcaagaag
    45121 tgttacaagt agcaaatgat catctccaac gtttaacgaa aaatcgttat caatttgagc
    45181 tagcggatac cattggcagt tatcgaggga aaaccggatt agaaatcaat atctatgatg
    45241 atcaggcagg tatgtctcga agtgcgcata ccctgtctgg cggtgagagt tttattgcag
    45301 cactttcttt agcattagct cttgccgatg tgatccagac acaagctggt ggtgtagcaa
    45361 tcgatgcatt gtttattgat gaaggatttg ggtcattaga tgaagaagct ttggaaatgg
    45421 caatggaagc tttagaaacg atcgaaagtg aaggaagaat gatcggcatc attagtcatg
    45481 taagagaatt gaaagaacga gtattacaac aaataagaat tgatacagaa ggtagcggac
    45541 aaagcaaaat acgctatcaa cttggataaa aagggagatt ggtaactatg aaatggagta
    45601 ttccagaaaa agtcattgca cgtggacgtc aatacttgca agaaggccga gtgttatcgg
    45661 ttgtccctga ccaagaagaa aacgtgtggc atgcagaagt cttgggcagt gaattgtata
    45721 tggttgattt ggatggtaca gcaaaagaga tagattactg ccaatgtccc tattgggagg
    45781 aacataaata ttgtaagcat acagttgctg tggaacttta tttacggtcg aaaaatctat
    45841 ctcgtatcat gaaaaaagac cagccaatgc tagaaccggt aaaagcaaca agtgagggag
    45901 aaatcattgc aaaaggtttt tctcgtctaa aaacacaaaa caatcctcaa gcggtgcaac
    45961 ctttgattat tgaataccaa gtagaaacga ttgagactaa tcagtatcac ccagagcttt
    46021 ctattttggc actttacttg cgtatcggtt cattggcatc accgaaaaaa acatatatcg
    46081 taaaaaacat ctatgatttt ttacaagctt acacagaaaa agaaacttac actgttaata
    46141 aacagtacca gtttcgtcta gataaacggg cgtttcaaaa agaagatcaa aaaatactca
    46201 cgcatcttgc aggaagtgct cagacgcatc aattattagg tacgaacggc ttacaagtca
    46261 aaggaaaact agataaaaga tatctgcttc tcccagtaga acaagcccgc ttcctgttag
    46321 agcagatgaa tcagactaca cgtttgcatc tgcagataaa tgaacaaaac gatcaaggaa
    46381 tacaattttc tgatcaaaac aaacctttat cattcaaagt ggaaaaattt ggagaagatt
    46441 atttgctgca agcattgaat gattttgatt ttttctttat gcattatcag tggggagtta
    46501 tccaagggac attttattct ctgacgaaat atcaacaaac ggtttatcaa acttggaaac
    46561 aattgattcg ccgattggag aaaccagaag tagtgttcaa gaaaaaagaa ttaccgtctt
    46621 tgtttaaaga gattatccct ctgctgtcag agatcggcga cgtagctatt gcccctgcag
    46681 tagaagagga agtagcagat atccccgtcg aatttatttt ctttttccga aaagcaaaag
    46741 gaaaaatcca agctcgagtc gatttcgtct atggtgaagt catctattcg actgacccga
    46801 agcatgaggt ctcgacagaa caggcttttc aggtgttacg tgacacagta gaagagaaac
    46861 gagtacttga tctattcaga atgtatcgct accagaaaaa tgaaactggt tatgaacgtc
    46921 cattgccaag cggggaagaa ctatatgcct ttttccgcac agaactatct gtcttccgtc
    46981 agtttggtga agtcagactt ggaagaaaat tgcgagagtt gtatttagat gcgcataaac
    47041 atcaaccgaa aatcgaagta accgagaccg gttcatggct cgatattcga ttcgatgtga
    47101 cagggataga ggagcaagaa atcgatcatg tcctgcaaag cttgctgaga aatgatgctt
    47161 tttatacatt agaaaatggt caggttcttt cttttgattc agaagaattt caacaaacaa
    47221 gccaagtgtt gcaacaatta cgggaatcga tacgtacaga agaaggaacg atccatgtac
    47281 ctaaaaatca aggattgatc attcaaaatc aattggaaaa aagcaatgct acttttagtg
    47341 aatcgttcca aacgatggta caagatctca ttcatccaga aagataccag gcacagcttc
    47401 ccaaaggatt gaatgcaacg atgagagatt atcaaaagca aggttttcga tggttgaaga
    47461 tgttaggtca ttatcagttt ggcggtattt tagccgatga aatgggactt gggaaaacac
    47521 tacagacaat tgccttttta ctttcggaaa aagaagaacg gaaaagcttc tctgctttga
    47581 tcgtagcgcc tgcgagctta atttacaact ggcaggcaga ggtgagaaaa ttcgcccctt
    47641 ctttaagtat ccaagtaatc aatgggaata aaaaagaacg tgaagaatta ctggctaaag
    47701 acacagatat ccgagtgact tcttatgcca gcttaagaca ggatctagct aattatcagt
    47761 cgcaaaaaat tgattatttg attcttgatg aggcacaaat ggtcaaaaat agcagtacga
    47821 aaactgcaca agcattgaga gaattagctg tacctcagcg gtttgcactg agtgggacac
    47881 caatcgagaa taatttagaa gaattgtggt cattgtttgc aacgattatg ccaggatttt
    47941 tcccgactaa aacaaaattc agggaacttg ccgcagaaga aattgcgcaa atgattcgac
    48001 cgtttatttt acggcgagat aaacaaactg tgttgaaaga cttgccggaa aaaacagaga
    48061 tgaatctata ttctgcactt acagaagaac aaaaaactgt ttatttagct tacttgcgtc
    48121 aaatgcgtga agaaatcaca tcaatggatt ctgaagcatt caagaaaaac cggatcggga
    48181 tacttgctgg tctgacaaga ctgcgacaaa tttgctgcga tcctcgtctg tttattgaag
    48241 attaccaagg cggttccggc aagctcgaac aagtaaaaga tctactggta gctgccaaag
    48301 agaacaaacg aagagttttg ctgttttctc aatttacagg gatgctgact atcttgcaag
    48361 aagagttagc cgagctaggg atctcgacat tttatttaag aggcagtacg aaaccacaag
    48421 atcggttaag tatggtagac gcatttaatg ctggcgaaaa agatgttttt ctgatttcct
    48481 taaaggctgg gggaactgga ttgaatctca cgggagcaga tacggtcatc ttgtatgacc
    48541 tttggtggaa tcctgccatt gaagaacaag ctgctggacg tgcacatcgg atcggacaaa
    48601 aaaatgtggt agaagtgtgg cgtatgatct ctgaaggaac gattgaggaa cgcatggatt
    48661 cactccagca agaaaaacgc gaactgttcc aaaaagtgat ccaaggtaat gaagaacagt
    48721 tgacgaaact gactgaagaa gacatccgca tgattttaag tgtaggagaa atcgaggagt
    48781 aagagactcg aaaaagcgtt cagtaatggg taagaaagcc tttctaaaga agcgattgtt
    48841 tatttgattt aaaaaatagc aaggaggctg ggattccatt ttatcccagc ttccttgcta
    48901 tcattttttt atttctattt ttaaaggtta tacaaagatt gtcttcattg tctctaacag
    48961 ctgatcaaaa gaagaaatct cgatatcgca aattggagtc tgttcaattt tgcgttctcg
    49021 atggttgaac caaagcgctt gccagttagc actttttgca cctagaacat cattgtcgta
    49081 attatcgcca acgtataagg tctcttctgg aagcatatga aattctttct cagccagctg
    49141 aaaaatctct ttttctggtt tttggaaacc tgttgcttga gaaatgagca tattgcggga
    49201 aggaatccaa ttgttcaatt gaagttgctt cagttttttt gtctgatggt cagtcggccc
    49261 atttgtgata attcctaatg ggacattctt ttccttcaag aaatcgaggg tttttttgac
    49321 ctcctcatgc aagcagatat tatcaagttc ttcctcgtaa attttttgaa ataagaggcc
    49381 atcttcttcc gtaatatgag ggtagtctaa atcttttagt gattggctga tccgatgtgc
    49441 tcgcatatat tctagtgtcc aatctcctgc cattactttt gggaagtttt catcactatg
    49501 atggcggaac cggatatata gtggatgcat atctgcatct gaaacaagcg gaacgactct
    49561 tttgaccgcg tttcgaaatg gctgctgctg atcatacatc gtatcatcaa catcaaacac
    49621 aactgattta atcaaaattt tacacgcctt tcggttttca ttgatttttt cacaaatcct
    49681 tga
//
LOCUS       DACDKM010000009        48959 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20834_2016
            SAMN12640370-rid9891663.denovo.009, whole genome shotgun sequence.
ACCESSION   DACDKM010000009 DACDKM010000000
VERSION     DACDKM010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640370
            Sequence Read Archive: SRR10040891
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 48959)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 48959)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742835.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742835.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:04:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,012
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 29 of 75
            Pseudo Genes (incomplete)         :: 33 of 75
            Pseudo Genes (internal stop)      :: 19 of 75
            Pseudo Genes (multiple problems)  :: 5 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..48959
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640370-rid9891663.denovo.009"
                     /isolate="E20834_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(59..1039)
                     /gene="pta"
                     /locus_tag="IV334_000539"
     CDS             complement(59..1039)
                     /gene="pta"
                     /locus_tag="IV334_000539"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HAP8942633.1"
                     /translation="MELFDSLRFKIVRRGIKIVFPEATDARILGAAARLKAEELVEPI
                     LVGNEEEIRNAAHARGIKTSSFTIISPDNYDKWDEMVDTFVERRNGKASKEQAEKILK
                     DVNYFGTMLTYMGIADGMVSGAIHSTGDTVRPALQIIKTKPGISRTSGAMIMVRGRDQ
                     EKYVFSDCAINVNPTAQELAEIAVDSAKTAELFDIEPKVAMMSFSTKGSAKSPEVDKV
                     VEATKIAKKLAPQYMIDGELQFDASYVPSVAQLKAPDSEVAGQATVFVFPELQSGNIG
                     YKIAQRFGNFEAIGPILQGLNKPVSDLSRGANEEDVYKLSIITAAQTLID"
     gene            complement(1192..1872)
                     /locus_tag="IV334_000540"
     CDS             complement(1192..1872)
                     /locus_tag="IV334_000540"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HAP8942634.1"
                     /translation="MKEIIHNSWQEVLSSEFSKDYYLHLREFLKKEYASQKIHPDMYH
                     IYEALELTPYEEVKVVILGQDPYHGENQAHGLSFSVQPGVKIPPSLRNIYKELYDDLG
                     IAPVQHGNLVSWAKQGVLLLNTVLTVREGQAYSHRGKGWERLTDTIIEKLNEREKPIV
                     FILWGKPAQEKIKMIDKSRHIIITSPHPSPLSASRGFFGSKPFSKTNDALLALGEEPI
                     DWQLPETV"
     gene            1998..2867
                     /locus_tag="IV334_000541"
     CDS             1998..2867
                     /locus_tag="IV334_000541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAP8942635.1"
                     /translation="MIKLIASDMDGTLLDAHMSVSPENAEAIRFANDAGVEFMVATGR
                     NYQEARAALDEVGIDCAMITLNGAQVFDKEGNSLFTVSIPNNQAISVLDILDANGIYY
                     EVATNDGLYSENQAKRIESFASMVATHLPHLTYKMAIAMASAKLELLHITYVDSIRAI
                     LEEKNLEVLKIICFHTEGPAVLGPVGKQISLLGDLAVTSSGQNNIEVNHKNAQKGIAV
                     AHVAHERGISLEEVMTIGDNFNDVSMLQVAGVSFAMGNAEIEVKDYAKYTTDTNLESG
                     VGKAILRAIAEDL"
     gene            2893..3408
                     /locus_tag="IV334_000542"
     CDS             2893..3408
                     /locus_tag="IV334_000542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8942636.1"
                     /translation="MSEFFIQEKQLGRATRTIVKNEKGHSLFLMVGRWGTKGDALSLY
                     AMNGDLVAHIKQISLTFGTRFELYKEFEKVGTMQKIFNWPGDFYYIRRLHWTVQGDIY
                     NHQYQIHHFNQQIMKMDKATLFSGDYYVLDVSNDEDAPVCICIAAVLDYWLYRKDKSR
                     DKPVSYRLNLS"
     gene            complement(3455..4018)
                     /locus_tag="IV334_000543"
     CDS             complement(3455..4018)
                     /locus_tag="IV334_000543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP8942637.1"
                     /translation="MIRFARKEDAPEIAPLILVILKDMELPFLKQTGEQKTLEILEEA
                     ITDPNYRYSYSRGIVEEIDGKVAGVAYGYTDEEESIIDQPLVPILEKYQLDASTRLFI
                     DKETFPNEWYLDSISVSEDFRGQGIGSRLLEALPKLAKKANRSVIGLSVDEKNPKAKK
                     LYERHDFKVVGQRMISGHLYDHMQKNI"
     gene            complement(4073..4252)
                     /locus_tag="IV334_000544"
     CDS             complement(4073..4252)
                     /locus_tag="IV334_000544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8942638.1"
                     /translation="MEKMKCPNCGKKFAYEEVNNVVEHNDKEMPVVCPYCRTEAARIV
                     THGYFITEKIEDFLK"
     gene            complement(4329..4838)
                     /locus_tag="IV334_000545"
     CDS             complement(4329..4838)
                     /locus_tag="IV334_000545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8942639.1"
                     /translation="MKKMMFGVAALIVIGITGMTGLLNAAFHMDDISDQEYRISLKKA
                     AAIVKNETKEHNISAIHFAVEKDLENPTNATYEYQFFCEGQVIAVNPSTGKTTVRNDT
                     EKAAEHLFDIEKTEEFKQPQAAMKEAVSYYGKKAKVKEWTLLVKDERPYYKVRLIDGE
                     QDRQVWISA"
     gene            complement(4934..5314)
                     /locus_tag="IV334_000546"
     CDS             complement(4934..5314)
                     /locus_tag="IV334_000546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HAP8942640.1"
                     /translation="MNLTTIHHIAIIVSDYQKSRDFYVNKLGFEVIRENYRKERDDYK
                     LDLKLGSAELEIFGIANAPQRPNYPEACGLRHLAFKVDDIEETIAELQSLGIESEPIR
                     EDTFTGKKMTFFFDPDGLPLELHE"
     gene            5644..6393
                     /locus_tag="IV334_000547"
     CDS             5644..6393
                     /locus_tag="IV334_000547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide mononucleotide transporter"
                     /protein_id="HAP8942641.1"
                     /translation="MKSYFQFLAHQLKGWPQQNYYLFFFSLGCQVMTLVNSPITWLSV
                     ITFIGTTLGVLCILSINAAKSVNGILGILSAICFIIVGFSAKNYLSIGEQIAYMITLD
                     IPVLLSKEWNHNMASKIRKFTGKSWFIAIISTFLVYLVSGFLIGRYTDDPRPWVDAIA
                     FSISLTAGIISFLRYNNQYYWWLASGLAQMVLWYISFTHGSASLAMFVNSSVYLLNDV
                     LAFTVSPWYNQKERERMQKMEEEYYNAQQHA"
     gene            complement(6577..7854)
                     /locus_tag="IV334_000548"
     CDS             complement(6577..7854)
                     /locus_tag="IV334_000548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8942642.1"
                     /translation="MEEWQSVFEEWFPKEISKSYPIKISKQYTSSQRWEIYAKLTKKQ
                     RELVDKHRRYLISSRFMEEHYLAATDWVFSDFKINPFFRTKRSQQKLYCECGRELKVQ
                     YIVKSPKTGKILKLGINHFADHLHVSPTVAASIHQGMTKVDLALDELLWLKQKNIDFP
                     EGLWQKYCFVLYQNRRMKQPYLPDIKLAQRLAEFRQVEMPIYIADYQALENEIKKISE
                     HINGQSKKRQIKKELFDDFAEELVKDVEEFLINYRAFLRKDWQSIVYEEVPVHPNAYF
                     ETFISVLRKTKRQRTPEVTAQMEYFAKNQRFIQPKIYLFIWKQYCRYGFTEGFFDSIP
                     RIVRNGFLKVLRKEREAIQSADKKDRTVSKEKWQLVVKDIQSGNVQETIDKWKGKHYR
                     FTEAQKQALEYYQKLEESLRFNDEARKYLKELL"
     gene            complement(7933..8577)
                     /gene="pcp"
                     /locus_tag="IV334_000549"
     CDS             complement(7933..8577)
                     /gene="pcp"
                     /locus_tag="IV334_000549"
                     /EC_number="3.4.19.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyroglutamyl-peptidase I"
                     /protein_id="HAP8942643.1"
                     /translation="MKVLVTGFDPFGGDKVNPAYEAVKKMPDEISGAEIIKVEVPTVF
                     EKSSQVVKEAIQQHQPDVVICVGQAGGRSAVSFERVAINLAEARIPDNEGNQPFDTAL
                     EENGPAAYFTSLPIKAMTKNVQDHGLPAYISYTAGTFVCNDIMYRLLHLIETQFPAIR
                     GGFIHVPFSPDQVIDRPVGTPSMSLEDIASSLTYAVEAAVENKEDISGNAGTTH"
     gene            complement(8776..9720)
                     /locus_tag="IV334_000550"
     CDS             complement(8776..9720)
                     /locus_tag="IV334_000550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF979 domain-containing protein"
                     /protein_id="HAP8942644.1"
                     /translation="MDFFTNPDILLGDKLLEILYIVMGLVLIYTGVRNLTDKTNPHRY
                     GSAFFWTVLGVVIAGGRWLPAIVNGGLIFAMTIPAIAKKVSKGESRLPSKEYMEKMSD
                     KLGMKIFIPALSIGVFAILFALFTNLGALVGVGVGVFAAMLILMFFSRDNRPSTFLDD
                     AADMLGTVGPLSMLPMLLASLGAVFTSAGVGTVISNAVGTIVPEGNVNIGIIIYALGM
                     VIFTVIMGNAFAAITVMTVGIGAPFVFSHGANPALIGMVALTCGFCGTLLTPMAANFN
                     IVPVAMLEMKDKYGVIKNQLFIALFMLVFQIAYMILFK"
     gene            complement(9722..10408)
                     /locus_tag="IV334_000551"
     CDS             complement(9722..10408)
                     /locus_tag="IV334_000551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF969 domain-containing protein"
                     /protein_id="HAP8942645.1"
                     /translation="MENYLSLLGVLIVILGFAFKLDSILIVMVALVVTALTGGLGIQG
                     MLELLGTSFVNNRGMAIFIIIMLATGTLERNGLKESAASLIKRFKKVSAGMIVDIYGI
                     FRMIFAAFNVSFGGVAGFVRPIILPMALGTIESKGLEVHPKHEEELKGMASAMENVCW
                     FFGQVLFIGGAGALLVQSTLKDLGYDVTLGQLALVEVPVAITALVVASVYFYLKEKKL
                     AKKYYPGKGQ"
     gene            10882..11202
                     /locus_tag="IV334_000552"
     CDS             10882..11202
                     /locus_tag="IV334_000552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux SMR transporter"
                     /protein_id="HAP8942646.1"
                     /translation="MAWMELIIAGILEVFWSTMMKWSDGFSKINYSFYTVIGMIASFY
                     FLSKAIKSLPMSLSYPIWTGIGAVGSVIIGVVLFHDKLNLSTWFFVGMLLISIIGIKI
                     TSGH"
     gene            11356..12261
                     /locus_tag="IV334_000553"
     CDS             11356..12261
                     /locus_tag="IV334_000553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAP8942647.1"
                     /translation="MKSLSVQELSKNFGHKQALSDVSFSLSPGKIVGLVGPNGAGKTT
                     IMKAILGLIHYSDGTICIGENEISPSSHKGLEQVGALIEYPGIYPFLTGYDHLKLFSE
                     TNDVSAIDTIVNQLKMEKYIHKKAKSYSLGMKQKLGIALALLNHPSIVILDEPMNGLD
                     PQATRDVRKIITDLATQGTSFLISSHLLSELEKMVDELILINQGKIIKQCTMAELNQT
                     AQDFIVISTTNDLAARTILTEAGYTLEDPDKIKLLKENEELLGTVIQLLSAQNIHVTD
                     VQHLTNDLESSLLEILDSVKGEEEK"
     gene            12258..13031
                     /locus_tag="IV334_000554"
     CDS             12258..13031
                     /locus_tag="IV334_000554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HAP8942648.1"
                     /translation="MTLLVKQELFKLIKKKSTAVLSVLLVVLLIGTALLAKKYTTIID
                     PVEMTAQLFSATSWIVFIMIAAASTIISMEAQYGTLKNLLYRKYSRGEILVSKWITLV
                     IYSVYLYLLAIIVTVLMKLILFPSISFTEKVSTGQTLIQSLFTYTLGSYIGLWLILSL
                     VLMIACFINSSGASISAGIVFYFASSIISGILIALIQKWEWIKWNPISMLNLQNQIGN
                     EEIMKQLTHLSTNQMLFGNLAYIVLFLALGYLVFKRKNV"
     gene            complement(13212..14636)
                     /locus_tag="IV334_000555"
     CDS             complement(13212..14636)
                     /locus_tag="IV334_000555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAP8942649.1"
                     /translation="MDTEQQKKGIGLIPLAALVIGSAIGGGVFGIMTDISGSAGGPAI
                     FSWVLVGTAMLMLSLSINNINKKRPDLKGGIFSYAEEGFGRFAGFISGWGYWLTCWLG
                     NVAFATLLMSALGYFFPVFEGGQNLASIVMSTVFLWGYAWLVNRGVENASFVNAIVTF
                     CKLVPLFLFIVVAISTFSIQQFIDNFSSDIILNANGQPIPFSSQVLSCIMVMLWVFVG
                     IEGASVVGSRAKKQSDVGKATILGIFGLIIIYVLVSMLPYGTMTMNQLQTIKAQPAMG
                     YVFEMMVGKWGAVVINGGLIISLVGVWLSWTILPIETMGNMAEDGLLPKAWGKLNKNG
                     APTYAIVLTTLCTNVFLLSLLFTDSAYNFAYTLGTAAIFFTWLFLGLYQMKLSYQRKE
                     WVQFLVGLLASAFQVWAMLVVAFNEVMLVLVLFLPGIYFYLKARKEQNEPVQHIHLDK
                     ILLIGVTMVGVIAMVLFATGMLHV"
     gene            14847..15293
                     /gene="nrdI"
                     /locus_tag="IV334_000556"
     CDS             14847..15293
                     /gene="nrdI"
                     /locus_tag="IV334_000556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HAP8942650.1"
                     /translation="MNILYISISGNTRAFAKHLAEYAEKMHAEDPVNPEVTLKEIHEN
                     SDFTKETEPFFTFVPTYLDGGNGLDNGDTEILTETMREYLEHEDNHKLCLGVVGSGNK
                     NFNNQYCLTAKQYAQTFGFPFLADYELRGTPSDVTRIYQILAENNK"
     gene            complement(15358..17340)
                     /locus_tag="IV334_000557"
     CDS             complement(15358..17340)
                     /locus_tag="IV334_000557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP8942651.1"
                     /translation="MAKNELTLEELAKQIYKGAGGMGNVESIVHCMTRVRMKIINDDL
                     VNVSELKAIPGVLGVVEDEQLQVIIGPGKVNKVAQQMVDLAGVKLGEKLSSVSTATAN
                     SSASGKDRVNERAQEMKAAQKSKQKPSKIKSVLKDISNIFVPMIPAFVGTGIVAGIAA
                     VLANLVTAGTLDAATWQQYIDVMNILKNGMFSYLVIYTGINAAQVFGATPTLGGVIGA
                     VVMLTGMNPEAPLKNLFTGEALAAGQGGILGVIFAVWLLSIVEKKLHKVVPDAVDIIV
                     TPTLSLIAIGLIEIFFIMPLAGFISDGMVGGINWVLGVGGAFAGFVLGTLFLPMVMFG
                     LHQILTPIHVQMIDETGRTLLLPILAMAGAGQVGAALALWIRCKKDKELTEMIKGALP
                     VGILGIGEPLIYGVTLPLGRPFITACIGGGIGGAVIGMIGNVGAIAIGPSGAALIPLI
                     SDGKWLGYILGLLAAYAGGFVATFFFGIPKDRLEKEELATETATVNEVPEKVVPQTSA
                     ESVTILSSVADGTVVSLDKASDPVFAQKMMGEGYFVEPENGHIYSPVTGVVSSVFPTK
                     HAIGITTPNGLEILLHMGINTVDLGGTPFDVKVTEGQQVTPDTLVAEADLEAIKAAGK
                     ETSMIVLVTNMDRVKNFVLEKTGKAKAKAPVMDVKA"
     gene            complement(17354..18250)
                     /gene="murQ"
                     /locus_tag="IV334_000558"
     CDS             complement(17354..18250)
                     /gene="murQ"
                     /locus_tag="IV334_000558"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HAP8942652.1"
                     /translation="MSKINLENLTTERRNQETFGLDEMSISQAVQLMNKEDQKVAAAV
                     EKELTSIEPVIANTIESFKKGGRLIYMGAGTSGRLGVLDAAECVPTFGVEPEMVIGLI
                     AGGEEAMTVAVEGAEDSYELGQEDLIKLNLTKNDIVIGIAASGRTPYVIGGLDYAKSI
                     GAVTGTISCNKQAEISKHADFPIEVDAGPEFLTGSTRLKSGTAQKLILNMISTISMIG
                     IGKVFNNLMVDVKPTNEKLIERSKRIIMQATEADYETAEKYFEEADQNVKLAIVMILT
                     NSDKEEAQAKLAEANGFVKGTL"
     gene            complement(18253..19314)
                     /locus_tag="IV334_000559"
     CDS             complement(18253..19314)
                     /locus_tag="IV334_000559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF871 domain-containing protein"
                     /protein_id="HAP8942653.1"
                     /translation="MFGFSIFMNDSLSEEKKQYIRQMASNGFVGIFTSMHIPEDDVSA
                     YQKRLIELGSEAKKNKLELMVDISGEALERAGFSLEDLTPLKAIGVTGLRMDYHISNQ
                     QIADWSHQLKISLNASTITPKDIDELKEAEADFSQIEAWHNYYPRPETGLDKEWYQKK
                     NQWLKIQGLLVQGFVPGDTELRGPLYQGLPTLEEHRGIHPLAGALDLLASNTDIVYIG
                     DAGLSENVQEQFASFQKEQTVLLHTEPVDEEFYEYILGKHTNRQDDARDVIRSVDARF
                     REIPPIPARNTATRMKGSITLDNEKYLRYMGEIQLTKYDLPADEKVNVVAKVIKEELP
                     LINQIKAGMNYQFIRKEGR"
     gene            complement(19525..20052)
                     /locus_tag="IV334_000560"
     CDS             complement(19525..20052)
                     /locus_tag="IV334_000560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HAP8942654.1"
                     /translation="MLYVTRHGETTWNAQGLVCGRADVPLTEKGQMQAQKLAEKVVDL
                     PVPITKIIHSPLQRARDTAQAVADRLSLPLTVDERLVEMDFGDYDGLPSKDENFQKAR
                     LAFAVRFPNGESVLDVYARIVPLLKECIEDEENVYLLVCHNALIRVINAYFHPMPNEG
                     FFTFMVDNTELVSYE"
     gene            complement(20119..21621)
                     /locus_tag="IV334_000561"
     CDS             complement(20119..21621)
                     /locus_tag="IV334_000561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Eat(A)"
                     /protein_id="HAP8942655.1"
                     /translation="MSKIEIKNLTFGYDSQGTLLFEQANLNFDTQWKLGLIGRNGRGK
                     TTLLNILQNKLPYQGQVIHQQEFAYFPQQTKDKERLTYYVLNDITDFEIWEIERELQL
                     MQTDPEILWREFSTLSGGEKTKVLLALLFVDDTHFPLIDEPTNHLDISGRKQVAAYLK
                     KKKQGFIVVSHDRGFIDEVVDHVLAIEKSQLELYQGNFSIYEEQKKLRDEFEMAQNEK
                     LKKEVSRLKKTAAEKAEWSRSREGDKTKKQVGFIDTESRRVNKGAVGADAARTMKRSK
                     AIVNRMETQISEKEKLLKDIEYIDSLTMNSQASHHKRLLSVEDLQLGYENLLFEPIHF
                     TIEPHQRVAISGPNGAGKSSIIHYLLGAFNGKVIGEKYQPKHLSISYASQNYEDNRGT
                     LAEFAEKNQVDYQAFLNNLRKLGMERDVFHNKIEQMSMGQRKKVELAKSLSQPAELYT
                     WDEPLNYLDVFNQEQLEQLILNVKPAMLLVEHDQTFLDKVSTEIISLERI"
     gene            complement(21879..23000)
                     /locus_tag="IV334_000562"
     CDS             complement(21879..23000)
                     /locus_tag="IV334_000562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HAP8942656.1"
                     /translation="MDILAAIDSFKGSASSFELNQAVLNGWEDRKGRKINVPIADGGE
                     NTAEAIYYALRGTWRTIDGMDLLFRQRKISYLLTTWQGKNTAVIESAQALGLDLLDKP
                     TDQTIRLASSYALGELIKDALLQEVEQIIVTLGGSGCSDGGLGLLQSLGASLEGYTEG
                     NPLLSTKKLHFDRRNVLVSKVKLIIAADVTNPYTGQQGAAWQFGRQKGGIDTTLAFLD
                     KQAGKIAQQIKDFWEVDLDDIPGSGAAGGIGGALFLLGAKMVSGFDLVSQITGLEKKI
                     EQADVIITGEGRIDQQTIHGKVPYGVAVLAKKYRKPIIAICGSRDQELEGLSDFLPIV
                     LSIHLGPIGLEEAMEHRQTLEKTKILGQTLSRFFTILPK"
     gene            23126..24067
                     /locus_tag="IV334_000563"
     CDS             23126..24067
                     /locus_tag="IV334_000563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HAP8942657.1"
                     /translation="MKIAIAGAGAMGSRIGLMLHQSGNEVLLIDRWPAHIEAIRTNGL
                     IADFNGKEVVAKLPIYSPEEIIESNEHVDLIVALTKANQLDDMFCSIQSIITDNTYVL
                     CLLNGLGHEDVLEKYVPKKNILFGITMWTAGLAGPGKVTLLGDGEIELENLEPEGEAF
                     TKKVVEVFQEANLNPIYSHNVRYSIWRKACVNGTLNGLCTILDCNIAELGAQKAAESM
                     VRTIVSEFASIAAKEGIILDQEEVYQHIASTYDPDNIGLHYPSMYQDLIKNHRLTEID
                     YINGAIWRKGQKYDIATPYCAFLTQLVHAKEGILGAE"
     gene            complement(24217..25113)
                     /locus_tag="IV334_000564"
     CDS             complement(24217..25113)
                     /locus_tag="IV334_000564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide biosynthesis protein Alg14"
                     /protein_id="HAP8942658.1"
                     /translation="MNKNDVLLVAKNKSLYFLPSIRSLDVWSGKDLLQSEFIKSARQQ
                     LANYQLIVFLDYGFDMEMARRVRPFTNAKIVLFFWNHFKAEHEAMLKQAQDEPAIDEI
                     YHFDFLEAKELGLKHNSSFYSKHMNFSSSEPKTDLFFGATNNGRKERAESYKQEFIKR
                     DVTTNYFILPHRGNEQPGYLTYEEYLKKTGESRGILELLREGQQGVTLRTFESMYFQK
                     KLVTDNQAIAHYHFYRPENIFLLRERDLDELPEFLHTPFKPILSELLEFYDAENWAKR
                     FITNDSTIYEQYEYDLSSVENK"
     gene            25382..26722
                     /locus_tag="IV334_000565"
     CDS             25382..26722
                     /locus_tag="IV334_000565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAP8942659.1"
                     /translation="MEDKKLKKQLSSRHITMLALGGAIGAGLFKGSGEAIGIAGPSVL
                     IAFLIGGLILYIVMKGLGKIVLSGGDTHHGLSGLIRPYLGAHSADFTDWVYWSMWIIN
                     IIAEAVAAASFLQLWFPHIPAWVFVFMLAVLTTIINLYSVRLFAETEYWLAFAKISVI
                     ILLIIFATYLVGQQMLGTGVFPTLQQLTDHGGFTPHGMKGIISSLLVVIYSYGGSELI
                     AITVSEADDPKRAIPKAIKGVMGRIISFYIIPLFLLLILFPWNTLAGTNVSPFVMVFE
                     KMNIPFAADIVNFVIVLALFSSINSGVYASSRILFFRLKDRKGSSRKLAVLNKHQVPQ
                     RAVLFCTSVLYLGVALSYFVGDKLFGYLAGSLSYTVLLIWIIISAASFVLALRKGTLW
                     DKGISFFALAVLGLIFLGILFTNSIGVTVLTGLLYLFIYFSYQKKNDAFVLTNE"
     gene            26940..27383
                     /locus_tag="IV334_000566"
     CDS             26940..27383
                     /locus_tag="IV334_000566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF305 domain-containing protein"
                     /protein_id="HAP8942660.1"
                     /translation="MKKYWKFLLMIGVSTVVMFGMMYLNVYALDHIFFSRTRVFMALM
                     MGAVMAVIMLLFMWKMYENKKINVMILAASLLVFAGSLFMVRSQTTVNDTAWMKAMIP
                     HHSIAILASKRADLKDPEVKKLAEEIIQAQEEEIDKMKKMIKEME"
     gene            complement(27887..29305)
                     /gene="nhaC"
                     /locus_tag="IV334_000567"
     CDS             complement(27887..29305)
                     /gene="nhaC"
                     /locus_tag="IV334_000567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HAP8942661.1"
                     /translation="MGKKGTPSFLEATLVLMVIVLGIAIGVVGLKVSPNITILAAIGM
                     VMLYAVIKRYPTDWLHEGIINGIKPGIIPIFIFILVGSLIAVWIQAGIIPTLMVIGFK
                     LISVQWFVPSVFVVCAIVGSAVGSAFTVMSTIGIAFFGIGTTLGLNPALVVGAIVSGA
                     VFGDKMSPLSESTNLAAAIVDADLFKHIKHMMWSTVPAFVVSFFLFMILGHTSKAASL
                     SSIREVTDVLEANFTISIWSLIPLFLMLLCAWKKVPAILTILLNIAVAVGMIFIQNPQ
                     TSLTKLAGVIESGFVSNTGNQQIDSLLSRGGIESMMPTVSLIILTLSLGGLLIEFGLI
                     STVMERVSRKMTNTPNLIFTTLMTSIGVNIFIGEQFLSVILPGNAFKETYEKAGFDPT
                     VLGRTLEDGGTVINYLVPWGIAGSFVASTFGIPTLTYLPFVFFSLLSPVFSMVSAFTG
                     WGISKKFVKPQEAVSKTAQLEQ"
     gene            complement(29708..30931)
                     /locus_tag="IV334_000568"
     CDS             complement(29708..30931)
                     /locus_tag="IV334_000568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HAP8942662.1"
                     /translation="MTTTISTNQLKDLIQYRLPEYQALALDIHEHPEVSNYEFYSSAA
                     LIKQLEKERFWVEKEVAGHRTAFDARYISNKPGPTIAFLAEFDALPGIGHACGHNLFG
                     TYSVLAASIVKQFIDETGGEIRVYGTPGEEGGENGSAKGSFVREGFFEDVDVALCVHP
                     AFRYGKTTESLANDPVDIKFYGVASHAAAAPEKGVNALEALIQVFNGINALRLQLPKD
                     VNIHGIITDGGVAANVIPDYAAGRFYLRAGNRQTLDKVYEKVENIVRGAAISTGTTFE
                     FGLFQNAVDDVIVTPSFDDLFFQHAKEAGVPDEEIETEQRTSLGSSDVGNVSQVIPTI
                     QPTVAISETYIAGHTEEFKAAAKSDKGLASIAIAAELLANTALDLLKEPKLLERIKQE
                     HQEIVARKKQEGATF"
     gene            complement(30962..31801)
                     /locus_tag="IV334_000569"
     CDS             complement(30962..31801)
                     /locus_tag="IV334_000569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HAP8942663.1"
                     /translation="MKKIIRFVVITFVAIIWLAGCSQGKADTTDKKETIVKLGIIGED
                     TDVWDNVKDRLKKENINLEYVKFTDYNQPNVALADGSIDLNSFQHQFFLDNYNKEHGT
                     DLVSIGNTVNAPLGIYSNTLKDVADIKDGAKVAIPNDVTNGGRALLLLQTAGLIKVDK
                     AKGQAPTVSDITENRKNLDISELDASQTARALSDVDISVINSGVAVDAGFNPASDAIF
                     LEPVDDHARPYVNIIVARKEDENNKTYQKIVDAYQTEDTVKVIEKTSKGSSVPAWETF
                     GRK"
     gene            complement(31818..32498)
                     /locus_tag="IV334_000570"
     CDS             complement(31818..32498)
                     /locus_tag="IV334_000570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP8942664.1"
                     /translation="MTDFFKTYLPNVYLIPDEFVEATKQTIYMSFWTAFIGGMIGIIL
                     GVTLVVTRPNGLLANRLLFEILDKLINIIRSIPFIILLSLLALTTRFLVGTTIGAKAA
                     LVPLIFGVVPFFARQIENALLEVDPGVIEAAEAMGTSPIGIILRVYLLEGLPGIIRVS
                     ALTIINVIGLTTMAGAVGAGGLGNLAISRGYNRFQTDVTIVATLLILILVFLSQFISN
                     QLIKKITH"
     gene            complement(32500..33573)
                     /locus_tag="IV334_000571"
     CDS             complement(32500..33573)
                     /locus_tag="IV334_000571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAP8942665.1"
                     /translation="MIELRDVSVCFKQKEKEIQAVDSVDLTIEKGDVYGIVGYSGAGK
                     STLVRVMNLLQKPTAGEVIVNQTNLSQLSPKELRKERKAIGMIFQHFNLMESRTIFDN
                     VDFSLKYSGKSKQERRQKVNELLELVGLEEKASAYPKQLSGGQKQRVAIARALANEPK
                     VLLCDEATSALDPKTTLQILALLKKLNRQLGLTIVLITHEMQVVKEICNKVAVMEDGR
                     IVEKGSSIQIFSNPEEELTKDFIRTATHLDQALETIIAHSAFADQIANKWLVELSYIG
                     NQTNEPLIAHLYSKYQVTANILYGNVELLQETPLGSLIVTLAGETKQRKKALDYLIKS
                     GVKINILQKNDLDSRIKVIEGGR"
     gene            complement(34220..34891)
                     /locus_tag="IV334_000572"
     CDS             complement(34220..34891)
                     /locus_tag="IV334_000572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HAP8942666.1"
                     /translation="MKGAIFDLDGLLVDTEKTYRDGWLWGFQQFDIHIPKEIVDAWGG
                     KNWKQSFDILVKAAGSPEKVQEVRAKREEYFYQQLTSGAIHLKPYAKEILADLKERHM
                     KLGIATTTVTKRATDILDHFKLADYLDTFTFGDEVSENKPSPIPYLTALERTGLEASS
                     AFAVEDSLVGATAASRAGLGVVLIPDSSFVRNYTVEEKEKLKLLAEGKDLRTVIEMLN
                     KKTEK"
     gene            complement(34978..37656)
                     /gene="mgtA"
                     /locus_tag="IV334_000573"
     CDS             complement(34978..37656)
                     /gene="mgtA"
                     /locus_tag="IV334_000573"
                     /EC_number="7.2.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium-translocating P-type ATPase"
                     /protein_id="HAP8942667.1"
                     /translation="MSFGHKGEIEMEKKKDTQKIYAAFAGKTKQELFELFHASANGLT
                     EEQIERSRENYGENAISYGKKTPFIVEILKAYITPFTLVLIALGIISFITEYVLAAPG
                     DKDLFGVAIIFTMVIVSGTMTLIQSVRSNQAAEKLKSLVKVTAAVKRNGKYTEIPMEE
                     IVCGDLVRLSAGDMIPADMRLLSAKDLFISQAALTGESYPVEKHAKVCEDDQQSETSY
                     ENLVFMGSNVISGSAEGIIVSVGNETLFGHVAETLSEKPIQSSFELGIHKTSMLLIKF
                     MSLMAPAVIVINGLTKGDWLEAFLFGLSVAVGLTPEMLPMIVTTNLVKGASVMAKKGT
                     VIKNLNAIQNFGAIDVLCTDKTGTLTQDKIILEYHMDCEGNEDDRVLRHAYLNSYYQT
                     GLKNLLDVAIIDEATETLDTDKINYQKVDEIPFDFERRRMSVVVADPAGKTQMITKGA
                     IEEMLNISKFIDLGGTVTPLTKERKEAVLAKVQSLNEDGLRVIGVAQKTNPSVVGEFS
                     VKDESDMVLIGYLAFLDPPKETTKQALKALKEHGVAVKVLTGDNELVTRSVCRQVGLE
                     INELVTGEKISEMSDHELAQVAEDHEVFVKLNPQQKARLTTALRKNGHTVGFLGDGIN
                     DAPAMKVADIGISVDTAVDIAKESADVILLEKDLTILERGLLSGRETFGNIMKYIKAT
                     ASSNFGNMFSVLIASTFLPFLPMLPLQLLFLNLIYDVSCISLPWDRMDKEYLDEPKKW
                     NASSIGEFMVYFGPTSSIFDITTYLLMYFVICPAVVGGSFHTLDSSQRVIFIALFHAG
                     WFVESLWTQTLVLHALRTPKVPFIQSNATFAMFVITTLGIIVGSVLPFTSFGAELGLM
                     PLPGNYWLWLFATVIAYLALVTAVKKIYIRRFQELL"
     gene            38315..38752
                     /gene="ndk"
                     /locus_tag="IV334_000574"
     CDS             38315..38752
                     /gene="ndk"
                     /locus_tag="IV334_000574"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HAP8942668.1"
                     /translation="MERTLVIIKPDGVRRHLVGSIIQRFEAKGLAIAEMKFETMTPEL
                     AKEHYQHLTERSFFDELIDYMTSGPVVYLVLVGEEVIDIVRKMVGATKAADAVPGTIR
                     GDYALPGTENIIHASDSRDAAVKEIARFFPPFDINKPSEKAAK"
     gene            complement(38774..39640)
                     /locus_tag="IV334_000575"
     CDS             complement(38774..39640)
                     /locus_tag="IV334_000575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAP8942669.1"
                     /translation="MYDGELIKKLRVNKKLTQSQLAEGICFKTSLVGIETNSVKKMSF
                     VTLRSFLERMNMTLAEYEWMRNQLDEPIKLKKKRRMLDRVQEDSFDPYKEISNNRRHF
                     KKTEDLYYLVLNLHMVEKTEVSETEFEFLRYECRIIEEYLMQIPDYGQFELSILADFP
                     WIFSDRFIENNYLKIKKRMRRLAQLNNHEQYLFTFLMNLTSFYIENGRLKKARSVNED
                     MKRSLAHKERSTVIYETLMADYYQRLISAALGEEKTKDYQEFFTVLEYMLGKNQRRSL
                     EKKLLAIAEPSN"
     gene            complement(39800..41893)
                     /locus_tag="IV334_000576"
     CDS             complement(39800..41893)
                     /locus_tag="IV334_000576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HAP8942670.1"
                     /translation="MTQLKKLCQNRLAIVLTAVFFFWLKTIIAYYADFSLGVEGTIQY
                     FILWINPIATTLLFFGLSLYIKKPKPALAILLIIDILNTLLLYLNIIFYREFTDFITV
                     KSVLGFSKVSQGLSGSSFSLMKPHDVIYWLDIAVFIGLLVWLKVKKIPIKSNPVGKPM
                     AIAVTCLSGLIFSGNLALSEANRPQLLQRTFDRSYIVKYLGIDAYTIYDGIKTGMTSS
                     VRAHASSNGIDEVLDYTKKHYAEPNPETFGIAKGKNVIVLHLESFQQFLINMKVDGQE
                     VTPFLNSIFQNQATISFDNFFHEVGQGKTSDAENMLETGTFGLPQGSLFTELGSDNVF
                     QAAPAILGQKQGYTSAVFHGNVASFWNRDHVYKNLGYDNFFDRSYFDESDETLGYGIL
                     DKDLFRESAQYLEHLQQPFYTKFLSVTNHTPYYTDDKNFDFPSLNTGNSTVDNYVRTA
                     HYLDQSLEQFFTYLKKSGIYQNSIFVIYGDHFGISNTDNKDLASALGKDPDTWDEFDN
                     AQMQRVPLMIHMPGYTKGTVNHEYGGEIDVLPTLLHLLGIDDKDYLHFGTDLLSSQHD
                     QVVAFRNGNFVTPKYTVLGGKAYNNQTGEIIDTKAAGIDEEISKDQEKVKTALSLSDK
                     LNQENLLRFYVPNGFETIDPKKYDYKNEAERNEAIEKQKGEHSTSVFSKNGNKTTTNL
                     YPVTRTDVEQQKNNQ"
     gene            complement(42642..43265)
                     /locus_tag="IV334_000577"
     CDS             complement(42642..43265)
                     /locus_tag="IV334_000577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8942671.1"
                     /translation="MRKKRPFLLFWLFGFFLLLILSAAFSGKMTADLAQKETEKIVAS
                     KESVDNFKKEFSTYWWAEYKITANYEKGELDIYLPKGKEENQFINIDDVLSAISLRQY
                     QFKDKHLTVLLLTHDGDKVARLKNGKVITAYSEKVDKKVVADRLAEWKKTNEQVKKTT
                     VSSQKNESTGTSPSDSGSGQTITGSEENQASIITNESSQGQYQSYGQ"
     gene            complement(43424..44821)
                     /locus_tag="IV334_000578"
     CDS             complement(43424..44821)
                     /locus_tag="IV334_000578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HAP8942672.1"
                     /translation="MKKYQFPANFWWGSAASGPQTEGRISGDGKGENIFDYWYKKEPQ
                     KFFDQVGPEKTSQVYTKYQEDVQLMKQTGHNSFRTSIQWSRLIPDESGTINEEAVRFY
                     HRYFDELIENGIEPFVNLYHFDMPMYLQEKGGWLNRETVEAYEKYAKSCFELFGSKVK
                     KWFTHNEPIVPVEAGYLYRWHYPEESDMKKAIQVGYHEALASALAIKSYHEMEQGGVI
                     GIILNLTPSYPRDESNPEDVKAAQIADAFFNRSFLDPAVKGEFPSELIEIVKELDLMP
                     VIHAEDLTVIKENTVDLLGINYYQPRRVKAKETPIDTKNGPMPEDYFDNYEMPGRKMN
                     PYRGWEIYEQGIYDILINVRDHYGNIDCFISENGMGVENEARFIKEDGMIHDDYRIEF
                     VQSHLKYVHQAIQEGANCLGYHMWTCMDNWSWTNAYKNRYGFISVDLAQDGKRTIKKS
                     GHWFKDVSDKNGFEA"
     gene            complement(44853..45824)
                     /locus_tag="IV334_000579"
     CDS             complement(44853..45824)
                     /locus_tag="IV334_000579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAP8942673.1"
                     /translation="MASIRDVAKMAGVSVGTVSRYLNGQQLKEKNMKKIAEAISALDY
                     KENIIAKGLKNNRSFSIGLLINGISSRFGSEVVSGIERVAEENGYSLLLSGFADQPEK
                     IEQKIEYLMKHVIDGLIVFLSEEEWSGFEMLTKMSIPVIALNCPEGPAGIDTILVNDR
                     ESVAKVIAHHGEQGHKKIGFIAATQTDYVARERLAGAKEAVQNDPSIQLEVFTGDYSR
                     KSGYYGAKALLEKGATAIFVSNYNMSIGAIECFNQEGVRIGKDIAFSHYDYLDKNDLS
                     ILPKITITPPTDKIGERAAEQLLKRIKKEEEENEKVIVMENIIHGIN"
     gene            46198..46623
                     /locus_tag="IV334_000580"
     CDS             46198..46623
                     /locus_tag="IV334_000580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8942674.1"
                     /translation="MKKLIVLFILSAGLLTLTGCDLFGGSDQSSDETTDSSVLKAEKA
                     DTTNSVYTLKVRKDMDLSAGQLTFTDKKMEWTRTYQTQGTDSSESSEEKTVLTDIKIT
                     TKDDEYIISGKENKKETTVIFTKIGNYRLKDEDGNIYSL"
     gene            complement(47111..47812)
                     /locus_tag="IV334_000581"
     CDS             complement(47111..47812)
                     /locus_tag="IV334_000581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HAP8942675.1"
                     /translation="MSVILISGTIGAGKSSLTDMLAKEIDSKPFYENVEDNEVLPLFY
                     SNPEQYAFLLQIFFLNKRFLAMKNALVNDDNVLDRSIYEDSLLFHLNADLGRVTDIEV
                     QQYDSLLDTMLNELDDVAPKKRPDLMVHIKVSLDTMLERIKKRGRDYEQLESDETLYT
                     YYETLNTRYNQWYEDFDVCPKIQVDGDKFDFVENPEDAKKVIRQIQEKLAELEGKPAG
                     KYFTDNQHAKGQELG"
     gene            complement(47828..48268)
                     /locus_tag="IV334_000582"
     CDS             complement(47828..48268)
                     /locus_tag="IV334_000582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deoxyribosyltransferase"
                     /protein_id="HAP8942676.1"
                     /translation="MSQIYLAGPFFSEEQIDRVSRIEKALEENKTVTSFYSPRHHQES
                     NYELFSAGWAQEVYEKDMEELTNAEFVVAILDFEHQTIDPGTAYELGVATMLKKPMII
                     VQEETVPTNLMITQSLHTYLKSDQAVREYDFETLPVETYVGEYL"
     gene            complement(48268..48843)
                     /locus_tag="IV334_000583"
     CDS             complement(48268..48843)
                     /locus_tag="IV334_000583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HAP8942677.1"
                     /translation="MKLTVFCGSRFGNKESYKFIAQTLGKYMAQEEIELVYGGSYSGI
                     MGVISKTVLENNGKVTGIYPNGLFETELPGKDVTTFIPTETIDERKVLLFEKGDAVLV
                     FPGGLGTLEEFSQLLSWIAIGLTPDKPIGILNIGGYYSGLQQLLETFAKEDFMDKKWL
                     DQVIFSNNPLELVNELRAVSIKNQLLLKEAK"
BASE COUNT        14146 a        10191 c         8578 g        16044 t
ORIGIN      
        1 aaaaaatgaa ctagtcaaga acagcagctg ctgtttttgg ctagttcgcg tgtatttgtt
       61 aatcaatcaa tgtttgtgct gctgtgataa tcgataattt atatacatct tcttcatttg
      121 ctccacgaga caaatcagaa accggtttgt tcaagccttg tagaattggt ccgattgctt
      181 caaagttacc gaaacgctga gcaattttat agccgatatt tcctgactgc aattctggaa
      241 atacaaagac tgttgcttga ccagctactt ctgaatctgg cgctttcagt tgtgcaactg
      301 aaggaacata agaagcatca aattgcaatt cgccgtcgat catgtattgt ggcgctagtt
      361 ttttagcgat tttagttgct tcaactactt tgtctacttc tggcgatttc gctgagcctt
      421 ttgtcgagaa gctcatcatt gctacttttg gttcgatatc gaacaattct gctgtttttg
      481 cagaatctac ggcgatttca gccaattctt gcgcagttgg attcacgttg atcgcacaat
      541 ccgaaaagac atatttttct tgatctcttc cacgtaccat gatcatcgct ccgcttgtcc
      601 gactgatgcc aggctttgtt ttgatgatct gaagtgctgg gcgaacagtg tcgccagtcg
      661 aatggatcgc tccagagacc attccgtctg caatccccat atacgtaagc atcgtaccaa
      721 agtaattcac atcttttaaa atcttttctg cttgttcttt ggatgccttt ccatttctac
      781 gttcaacaaa agtatccacc atttcatccc acttatcgta attatcaggg ctgatgatcg
      841 taaagctaga tgttttgatt cctctggcat gcgctgcatt tcttatttct tcttcgttcc
      901 ctactaaaat gggttccaca agttcttctg ctttcaaccg ggcagctgcg cctaaaatac
      961 gagcatcagt agcttccggg aaaacgattt tgatcccacg gcgaacaatt ttaaatctta
     1021 aactatcaaa caattccaca agatgacctc cagacgtttt tatatgtatt atcatacact
     1081 attccagaaa aaaagaacag tttattttat ctgtatcagt caaaaatgct ggaaaaatta
     1141 agtttttcac atacaaagcg aactgcacta taacaggaaa gcgctgtttt attacactgt
     1201 ttcaggcagt tgccaatcaa tcggttcttc tcctaacgca aggagcgcat cattcgtttt
     1261 cgagaagggt ttggaaccaa aaaagccacg acttgcagat aacggactgg gatgtggaga
     1321 tgttatgatg atatggcgac tcttatcgat cattttgatt ttttcttgtg ccggttttcc
     1381 ccacaagata aacacgattg gtttttcccg ttcatttaat ttttcaatga tcgtgtctgt
     1441 taatctttcc caacctttcc cgcgatgaga atacgcttgc ccttcccgaa cggtaagaac
     1501 ggtgttgagt aaaaggactc cctgtttagc ccatgacact agatttccat gttgaacagg
     1561 tgcgattcca agatcatcat aaagctcttt atagatattt cttaacgatg gcgggatctt
     1621 tacgccaggt tgcacagaaa aagataagcc atgagcttga ttttctccat gataaggatc
     1681 ttgtcctaaa atcaccactt taacttcttc ataaggagtc aattccaatg cttcatagat
     1741 atgatacata tctggatgga tcttttggga cgcatattct ttttttaaaa attctcttag
     1801 atgcagataa taatcttttg aaaattctga ggacaaaact tcttgccaac tgttatgaat
     1861 gatttctttc aaaaactcca cctcctaata aaaagaataa ctcacatttt cttcaatcac
     1921 aagggacaat cttcaccctc agcacttgat taatatggta aactaggaac aagaagcaga
     1981 aaggaacgat aatcattatg atcaaattaa ttgcatcaga tatggacggc acattattag
     2041 atgcccatat gagtgtttct cctgaaaatg ccgaggcgat acgttttgca aatgatgccg
     2101 gtgttgagtt catggtcgct actggtcgaa actatcaaga agctcgcgca gctttagatg
     2161 aggtaggaat cgattgtgcg atgatcactt tgaacggggc acaggtattt gataaagaag
     2221 gaaattcatt gtttaccgtg tcgattccaa ataatcaagc aatctctgtt ttagatattc
     2281 tagatgctaa cggtatttac tatgaagtag caaccaacga tggtctttat tctgaaaatc
     2341 aagcgaaacg aattgaaagt tttgcttcca tggtggctac ccatttacct caccttacgt
     2401 ataaaatggc gattgccatg gcctcagcta agctggagtt attgcacatc acatacgtgg
     2461 acagtattcg tgctattttg gaagaaaaga atctggaagt tttaaaaatt atttgttttc
     2521 atacagaagg gccagctgta ttaggtccag ttggaaagca gatttctctt ttaggcgatc
     2581 tagccgttac ttcctctggt caaaacaata tcgaagtgaa ccataaaaac gctcaaaaag
     2641 gaattgctgt tgcccatgtc gctcacgagc gtgggatttc tttagaagaa gtcatgacga
     2701 ttggcgataa cttcaatgat gtcagcatgc tgcaagtagc aggagtcagt ttcgcaatgg
     2761 gaaatgcaga aatcgaagta aaagattatg ccaaatatac aacggacaca aaccttgaat
     2821 ctggcgtagg aaaagccata ttgcgcgcga ttgcagaaga cttgtagttt tatctagaaa
     2881 agagggatat agatgtcaga atttttcatt caggaaaagc agcttggacg tgcaactcgg
     2941 acaattgtca aaaacgaaaa aggacattcc ctgtttttga tggttggtcg ctggggaaca
     3001 aagggcgatg cattgtctct ttatgccatg aatggcgatc tcgttgctca catcaagcag
     3061 ataagcctga cattcggtac gcgttttgag ctgtataaag agttcgaaaa ggtgggaacc
     3121 atgcagaaaa tattcaattg gcccggtgac ttttattata ttcgtagact tcactggaca
     3181 gtccaaggtg atatctataa tcatcaatat caaatccatc atttcaatca gcaaatcatg
     3241 aaaatggata aagcaacact tttttcagga gattactatg tattggatgt ctcaaatgat
     3301 gaagatgctc cagtttgtat ttgtattgct gcggttttag attactggct ctaccgtaaa
     3361 gacaaaagtc gcgataagcc tgtttcttac cgacttaact taagttaaaa agatgtacct
     3421 gaaatgcgga aatacgcatt tcaggtacat ctttttagat attcttttgc atatgatcat
     3481 aaagatgacc actgatcatc cgctgaccga caactttgaa gtcatggcgt tcgtataatt
     3541 ttttagcctt tggatttttt tcatccacac ttagaccgat gacggagcga ttagctttct
     3601 tcgccaattt aggtaatgct tctaataatc gcgaaccgat gccttgtcct ctaaaatctt
     3661 ctgatacact gatagagtcc aaataccatt cgttgggaaa cgtttcttta tcaatgaaca
     3721 aacgtgtaga cgcatctagc tgatatttct ctaagatagg taccagtggc tgatcgatga
     3781 ttgattcttc ttcatctgta taaccgtaag caactcctgc aactttaccg tctatttctt
     3841 ctacgatacc tcgtgaataa ctgtaacgat aatttggatc agtgattgct tcttctaaaa
     3901 tctctaaagt cttttgttcc cctgtctgct tcaaaaatgg cagttccata tcttttagaa
     3961 tcactaagat caatggggcg atttctggag catcttcttt tctcgcaaaa cgaatcatag
     4021 tttcctctcc ttttctccat tctaacatgc agaaaaagaa atcgcaaaat cgttatttta
     4081 aaaaatcttc aatcttttca gtaatgaaat aaccatgagt aacgattctt gccgcttcag
     4141 ttcggcaata agggcagacg actggcattt ctttatcatt atgttcgact acattattga
     4201 cttcttcgta tgcaaatttt tttccgcaat tagggcattt cattttttcc atgttatctc
     4261 aacttccttt catctttact ctcttagctt actcttatat tgtaaaaaag agaagcaata
     4321 tccttcttct atgcagatat ccatacctga cggtcctgtt ctccatctat taatcggact
     4381 ttataataag gacgttcatc tttgactaga agtgtccact ctttcacttt tgcttttttc
     4441 ccgtagtagc tgactgcttc cttcatcgct gcttgcggtt gtttgaactc ttccgttttt
     4501 tctatatcaa aaagatgttc tgcggccttt tccgtgtcat ttcgtacggt tgttttccca
     4561 gtggatggat tgactgctat gacttgtcct tcacaaaaaa actggtattc atatgttgca
     4621 ttcgtaggat tctccaaatc tttttcaact gcaaaatgaa tagcagatat gttatgttct
     4681 tttgtttcgt tttttacgat tgctgctgct tttttcaatg agatccgata ttcctggtca
     4741 ctaatatcat ccatgtgaaa agcggcattg agcagtcccg tcataccggt aatccctatt
     4801 acgatgaggg cagcaacacc aaacatcatt tttttcattc ttcattctcc tgtttctttt
     4861 ttttaaagaa tatcaagaaa tgaacgaatg aaaaatcgaa tgaaacgaaa aaaaagtgac
     4921 aattttgtta tctttattcg tgcagttcta atggcaatcc gtctggatca aagaaaaacg
     4981 tcattttttt tcctgtaaag gtatcttctc taatcggttc tgattcgatc cctaaggact
     5041 gcaattctgc aattgtttct tcaatatcgt ctactttgaa agcaagatga cgcaagccac
     5101 aagcttctgg atagtttggt cgttgtggcg cattagcaat tccaaaaatc tccaattcag
     5161 cacttcctag tttcagatct aatttatagt catctcgttc tttgcggtaa ttttctctga
     5221 ttacttcaaa tcctaattta ttaacataaa aatcacgtga tttctgataa tctgaaacaa
     5281 tgatggctat atgatggatc gttgtaaggt tcatatgagc actcctattc ttcgtttaag
     5341 ctcggatcta tattaatctc cagttccatt gtaaatgtgt cagattgaaa aaaatagctg
     5401 tttttccttt ttataagaaa aaaggcaaaa aaaacagaaa gtaggtcctc caagacactc
     5461 ttcctgttcc caatataata aatgaaagta agaaattata ttttcaagaa aacaaacaga
     5521 aaattatcat aacacaaatt atttataatt gaaagactag ttttttttca agaaaagcat
     5581 acaatagatt gtgttagtaa atacagaaca cactacattt agtgtcttaa ggagtcctca
     5641 tacatgaagt catattttca gtttttagct catcagctaa aaggctggcc gcaacaaaat
     5701 tattatcttt tctttttcag tcttggctgc caggtcatga ctcttgtcaa tagtcccatc
     5761 acttggctgt ccgttattac atttatcggt accacgcttg gtgtattgtg tattttatcc
     5821 atcaatgcgg caaaatcagt caacggtatt ttaggtattt tgtctgcgat ttgttttatt
     5881 atcgttgggt tttcagcaaa gaactattta agtatcggtg aacagattgc ttatatgatc
     5941 acattggata tccccgtatt gttaagtaaa gaatggaatc ataatatggc ttcaaaaatc
     6001 cgaaaattca ctggaaaaag ctggtttatc gcaatcatca gtactttcct tgtctatctt
     6061 gtttcaggat tcctgattgg tcgttataca gatgatcctc ggccgtgggt agatgccatt
     6121 gctttttcta ttagcttgac tgctggtatc atcagtttct tgcgttacaa caaccaatat
     6181 tactggtggt tagcttcagg gcttgctcaa atggtccttt ggtatatttc atttacgcat
     6241 ggatctgcaa gtttggcgat gttcgtgaac agctctgttt acttgctgaa tgatgtcctt
     6301 gcatttaccg tttcaccttg gtataaccaa aaagaacgtg aacgtatgca aaaaatggaa
     6361 gaagaatact acaacgcaca acaacacgca taaaaaaact gagcgtggga caaaagtaaa
     6421 gaacactttt gtctcacgct ctaaacacgt ataaacggcg gaatcagaag caactccttc
     6481 ggaaataagc tgaaattcac aaaaatttga aaagcaattt tcgtgaattt cttcttattt
     6541 cttggagtta aacgcttctg tcccagcctc tattttttat agtaattcct tcagatactt
     6601 tcttgcctca tcattgaaac gcagtgattc ttctaacttc tgataatatt ccaacgcttg
     6661 tttctgagct tcagtaaaac gataatgttt tcctttccat ttgtcgattg tctcttgcac
     6721 atttccagat tggatatctt tcactactag ttgccatttt tcttttgaga ctgtacggtc
     6781 tttcttatca gcagattgta tagcttctcg ttctttacgc aatactttta aaaaaccatt
     6841 tctgactatc cgaggaatgc tgtcgaagaa cccttctgta aaaccatagc gacaatattg
     6901 cttccaaata aatagataga tttttggttg gatgaatcgt tggtttttcg caaaatattc
     6961 catctgagca gtgacttctg gtgttcgttg acgttttgtc tttctcaaca cggaaataaa
     7021 tgtttcaaaa taagcattag gatgtactgg cacttcttca tatacgatgg attgccaatc
     7081 tttacgtaaa aatgcgcgat agttgatcaa gaattcttct acatccttta ctaattcttc
     7141 tgcaaagtcg tcaaatagtt ctttttttat ctgtcgcttt tttgactgcc cattgatgtg
     7201 ttcacttatt ttcttgattt cattttccaa tgcttgataa tccgctatgt aaatcggcat
     7261 ttctacttgg cgaaattctg cgagccgctg agctaatttt atatccggta aatatggctg
     7321 tttcatccga cgattttggt acaagacaaa acagtatttt tgccatagtc cttcaggaaa
     7381 atcgatattc ttctgcttaa gccataataa ttcgtctaag gccagatcaa cttttgtcat
     7441 tccctgatgg atcgaagctg ccactgtcgg agagacatgc aggtgatcgg caaagtggtt
     7501 gatgcctagt tttaggatct ttccggtttt tggcgattta acgatgtatt gcacttttaa
     7561 ttctctacca cactcacaat acaatttttg ctggcttcgt tttgttcgaa aaaacggatt
     7621 gatcttgaag tcagaaaaca cccaatctgt tgcagctaaa taatgttctt ccataaaacg
     7681 agagctgatt agatatcggc ggtgcttatc gactagttcc cgctgttttt tcgttaattt
     7741 tgcatagatc tcccaacgtt gactgcttgt gtattgttta gaaattttga tgggatagct
     7801 tttgctgatt tcttttggaa accattcttc aaagacggat tgccattctt ccacggctgt
     7861 ttctttcctt tctaattaca aaaagctggc tactaacata acgttagcaa ccagccagtg
     7921 tttcttgcta aattagtgag ttgttccggc atttccggaa atatcttctt tattttctac
     7981 tgctgcttca actgcataag tcaaagaaga agcaatgtct tctaaagaca tagaaggcgt
     8041 acccactgga cgatcgatca cttgatcggg tgagaatggc acgtggatga acccacctcg
     8101 aattgccggg aattgtgttt cgatcaaatg aagcaaacga tacatgatat cattgcagac
     8161 aaatgttcct gctgtataag agatgtacgc tggtagacca tgatcttgta cattttttgt
     8221 cattgctttg attggcaatg aggtaaagta agcggctgga ccgttttctt ctaaagccgt
     8281 atcgaatggc tgatttcctt cattatcagg gattcttgct tctgctagat tgattgcgac
     8341 acgttcgaac gagactgctg accgcccacc tgcttgtccg acacagatca cgacatctgg
     8401 ttggtgctgt tggatggctt ctttgactac ttgactgctt ttttcgaata cggtcggtac
     8461 ttccactttg atgatttcag caccgctgat ttcatctggc atttttttta ctgcttcata
     8521 ggctggattc actttgtccc caccaaatgg atcaaaacct gttactagta ctttcatctg
     8581 ttgttcctcc gtccgattga tttaacatat ggttatcttt tatcatacca ttttccttga
     8641 acagcgtaaa taaaggaaac aatagtttaa ataacataac agatcaaact taatatattc
     8701 ttataaaaaa atagtgttcc actacaatgt tagaagaggt tgcgacaatt attctgtcac
     8761 aacccctctc ttctattatt taaatagaat catataagca atttggaaaa ctagcataaa
     8821 taacgcaata aacaactggt ttttgatcac tccgtatttg tctttcatct ccagcatcgc
     8881 tacaggcacg atattgaagt tagcagccat cggtgtcagc agggtcccac aaaaaccgca
     8941 agtcaatgca accattccta ttaacgctgg attcgctcca tgactaaata cgaatggtgc
     9001 accgatccca accgtcatca cggtgatggc cgcaaatgca ttccccatga ttaccgtaaa
     9061 tatcaccatg ccaagagcgt aaatgatgat gccgatgttc acatttcctt ctggaacgat
     9121 cgtacctact gcattggaaa tcactgtccc aactccagcg cttgtaaata ccgcacctag
     9181 agaagctaaa agcattggca acatgcttaa cggccctact gtgcccagca tatctgctgc
     9241 atcatctaaa aatgtacttg gtcgattatc tcgagaaaag aacatcaaga ttagcatggc
     9301 tgcaaaaaca ccgacgccca cgccgactaa agcacctaaa ttggtaaata gagcaaacaa
     9361 gatcgcaaag acaccgatac tcaaagctgg aataaaaatt ttcattccta gtttgtctga
     9421 cattttttcc atatattctt ttgatggaag ccggctttct cctttgctga ctttcttagc
     9481 aattgctggt atggtcatcg caaagatcaa gccaccgttc acgattgccg gaagccatct
     9541 tcctcctgca ataactaccc ctaagaccgt ccagaaaaag gctgacccat aacgatgagg
     9601 attggtttta tctgttaaat ttcttactcc agtgtaaatc aatacgagtc ccatcacgat
     9661 ataaaggatc tccagtaatt tatcgcctaa taaaatatca ggatttgtaa aaaagtccat
     9721 cttattgccc ctttcctgga taatatttct tcgctaattt cttttcttta agatagaagt
     9781 agacacttgc cacaactaaa gccgtgatag ccactggaac ttccactaaa gcaagctgtc
     9841 ccaatgttac gtcatatcct agatctttca gtgttgattg gactagcaac gcccctgctc
     9901 ctccgatgaa tagtacttgg ccaaagaacc agcagacatt ttccatagct gaagccatac
     9961 cttttagttc ttcttcatgc ttaggatgga cttctaaccc ttttgattcg atggtcccca
    10021 atgccatcgg taaaatgatt ggacgaacaa atccagcaac tccaccaaaa ctcacgttaa
    10081 aagccgcaaa gatcattcga aagatcccat aaatatctac aatcatacca gcagatactt
    10141 tcttgaatcg cttgatcaag gaagccgctg attctttcag accatttctt tcaagcgttc
    10201 ctgttgccaa cataatgatg ataaaaatcg ccatcccgcg attgtttaca aaacttgtac
    10261 ctaaaagttc taacattcct tgaatgccaa gaccgccagt caaagctgta acaactaaag
    10321 caaccattac gatcaatatc gagtctaatt taaatgcaaa cccgagaata acaatcaaga
    10381 cgcctaaaag gctaagataa ttttccatat attttcctcc ataaaatgac aatatttttt
    10441 taattataaa gcaaaacaca taaaaaatat agttgttttc tcacgaaata gacactattt
    10501 ttaaatattt ttcgttattt ttttatcaca aaaattatct tacttattgt aaggtaaagc
    10561 tttttttaat ttattgcccg ataatatata gaaataaggt ctagttactg ttttttttaa
    10621 atgattaata aataacaact tctttctatg aaattgcaaa gcattcatct tcttttccgc
    10681 taacgatata tccgtcagtg cttggtacac tttactaaaa agtatgagat tgccgatatc
    10741 tggataggtg tgctatacta cagaaaaaga tatacaatct gccaccgggt aggataaaga
    10801 ggatgatcat tccattaggt ctttgaaggg atgattttcc tctttttgtg tattttttta
    10861 tagttaagga ggaatgtaag tatggcatgg atggaattaa tcatagcagg aattttggaa
    10921 gtattctggt caacgatgat gaaatggagt gatggttttt ctaaaatcaa ttattctttt
    10981 tacacagtga ttggcatgat tgctagtttt tactttttgt caaaggcaat caaaagtttg
    11041 ccgatgagtc tctcttatcc tatctggaca gggattggcg cagttggttc ggtcataatt
    11101 ggtgtggtct tgtttcatga taagctgaat ctttccactt ggttctttgt ggggatgcta
    11161 ctcatcagta tcattggcat caagattact agcggccact agacacttgg aataagtact
    11221 gatcagaaaa aataagcctt tttatttaat attcaatttt tttatgaaaa aataagaaat
    11281 aagtccaatg acctagcgac ttctccactc atcaggtgta tgcttgtact atctatagaa
    11341 aaggagtgaa tatttttgaa aagtctttcg gtccaagaat tatccaagaa ctttggtcac
    11401 aaacaagccc tcagcgatgt cagttttagc ttatcccctg gcaaaatcgt tggattagta
    11461 ggtcctaatg gcgctggaaa aacaacgatc atgaaagcta ttttaggact gatccattac
    11521 tcagatggaa caatctgtat tggtgaaaat gagatttctc cctctagtca taaagggttg
    11581 gaacaagtag gagcattgat cgaatacccg ggtatctatc cattcttaac aggctatgat
    11641 catctgaagt tattctctga aaccaacgat gtatctgcta tcgacactat cgtgaatcag
    11701 ctcaagatgg aaaagtacat tcacaaaaaa gcgaaatcct actcgttggg aatgaaacaa
    11761 aaattgggaa ttgctcttgc cttactgaac catccgtcta tcgttatttt agatgagccg
    11821 atgaacggtt tggatccaca agcaacaaga gatgtgcgaa aaatcattac cgacttggct
    11881 actcagggca cctctttctt gatctccagt cacttactca gcgagcttga aaaaatggtc
    11941 gatgagctga tcctgatcaa tcaaggaaag atcatcaaac aatgtacaat ggctgaattg
    12001 aaccaaacag cgcaagattt catcgttatc tctacaacaa atgatctagc tgctcgaacg
    12061 atcttgacag aagctggata tactttggaa gatcctgata aaatcaagct tttaaaagaa
    12121 aacgaagaat tattaggaac cgtgatccag ctattgtctg cacaaaatat ccatgtgaca
    12181 gacgtccagc atctgacgaa tgatttggaa tcttctctat tggaaattct ggattcagta
    12241 aagggggaag aagaaaaatg acattgcttg taaaacaaga attatttaaa ttgatcaaga
    12301 aaaagtcaac tgctgtttta tctgttctat tagttgtttt gctcatagga acagcacttc
    12361 tagccaaaaa atacacgacg atcattgatc cagttgaaat gactgctcag ctttttagtg
    12421 ccacatcttg gattgtcttt atcatgattg cagctgcaag cacgatcatt tcgatggaag
    12481 cacaatatgg tacattgaaa aatctccttt atcgaaagta ttctcgagga gaaatattag
    12541 taagcaaatg gatcacatta gttatttatt ctgtttattt atacctttta gctattattg
    12601 tcactgtgtt gatgaaactg attcttttcc cttcgatttc cttcacggag aaggtttcta
    12661 ctggtcaaac actgatccaa agcttgttca cttatacttt gggtagttat atcggtttat
    12721 ggctgatttt aagtttagtg ttgatgattg cttgctttat caatagttcc ggcgcatcca
    12781 tctcagcagg aatcgtcttt tattttgctt cgtcaatcat atctggtatc cttattgcac
    12841 tcatccaaaa atgggagtgg atcaaatgga atccgatcag tatgttgaat ctgcaaaacc
    12901 aaattggaaa cgaagagatc atgaagcaat tgactcatct ttctactaat caaatgctat
    12961 tcggtaattt agcttacatc gttttgttct tagcattagg atatctcgta tttaagcgaa
    13021 aaaatgtatg atgactcatc aaaaaagagg ctgtgaaaga gacgttcagc cttattgccg
    13081 attggctgtc tcttaaacac cgtttattct gaaaaaggag ctgtgacaac atttgtgtca
    13141 cagctccttt cctagtatgt ttttgaataa acgacaggct cacaacctct ctttatactt
    13201 cttttatact tctatacgtg aagcatccca gtggcaaata aaaccattgc aatcactccg
    13261 accatcgtca cacctataag taaaatctta tctaagtgga tatgttgtac aggctcgttt
    13321 tgctccttac gtgctttgag gtaaaaataa atgcctggca agaaaagaac aagaacaagc
    13381 attacttcat taaaggctac gactagcata gcccatactt gaaaggcact tgctaaaagc
    13441 cctactaaaa attggaccca ttctttccgc tgataagaaa gtttcatttg gtacaatcct
    13501 aagaaaagcc acgtgaagaa aatagctgct gtaccaagtg tgtaagcaaa gttataagca
    13561 ctgtcagtga ataataatga aagtaaaaag acattcgtac ataacgtcgt caatacgata
    13621 gcataagttg gtgccccatt tttgttgagt tttccccatg ctttaggcaa aagtccgtct
    13681 tcagccatgt ttcccattgt ttcaatcggt aagatcgtcc atgagagcca aacaccaact
    13741 aaagaaatga tcaaaccgcc gttgatcacg actgctcccc atttaccaac catcatttca
    13801 aacacatagc ccatagcagg ctgcgccttg atcgtttgca gctgattcat cgtcattgtc
    13861 ccatatggaa gcatggaaac caagacgtaa ataataatca agccgaatat ccctagaatc
    13921 gttgctttcc caacatctga ttgttttttt gctcgtgaac ctacgacaga ggctccttca
    13981 ataccaacaa atacccaaag catcaccatg atgcaactca atacttgact ggaaaacggg
    14041 atcggctgtc cattcgcatt caatataatg tcgctagaaa agttatcaat gaattgttgg
    14101 atcgaaaacg tgctgatagc tactacaata aataaaaata atggcactaa tttacagaat
    14161 gtgacgatag cattgacgaa actagcattt tcgactcccc gattcactag ccatgcatat
    14221 ccccataaaa agactgtact catcacgatc gatgccagat tttgcccacc ttcaaatact
    14281 gggaagaaat atcctaatgc gctcatcaac aatgttgcaa aagctacgtt gcctaaccag
    14341 catgtgagcc agtaacccca accgctgata aaaccagcga aacggccaaa accttcttct
    14401 gcgtaactaa agatcccgcc ttttaaatct ggacgttttt tattgatatt attgatcgac
    14461 aaggagagca tcaacattgc tgttcctacg agtacccaac tgaaaatagc tgggccgcca
    14521 gccgatccgg agatgtccgt cataatacca aatacgccac ctccaatcgc tgaaccaatt
    14581 actaaggcag caagtggaat taaaccaatt ccttttttct gttgctctgt atccatgtaa
    14641 gcatcctcca aaataaaacc gcttttatca aaaagtataa caaacctttt ttatttttac
    14701 ttaattaata aaaactttaa tagaaatata attctaccgg cttttttctc attatacaag
    14761 caaagcgcct agtccctttc caaattacac caccaaaaaa cttaaaaaag atgctatact
    14821 aaaaggaaag aattggagga tgatccatga acattttata tatatcgatt tcaggaaata
    14881 cgcgtgcctt tgccaaacat ctagcagaat atgctgaaaa aatgcatgca gaagatcctg
    14941 tcaatcctga agttacttta aaagaaatcc atgaaaatag tgattttacg aaagaaacag
    15001 aaccattttt tactttcgtt cctacttatt tagacggcgg aaatggttta gataacggtg
    15061 atacagaaat cttgaccgaa acgatgcgtg aatatctgga acatgaagac aatcataaac
    15121 tttgtcttgg tgtagtcggt agcggaaata aaaacttcaa taatcagtat tgtctaacag
    15181 ccaaacaata tgcacaaaca tttggctttc ctttcttggc agattatgaa ttacggggaa
    15241 caccatctga tgtgacacga atttatcaga ttttagcaga aaacaataaa taataaaaaa
    15301 gaagaagctg aaaaatagat ttcagtttct tctttcgttt ctattgtttc tatcatatta
    15361 agctttgaca tccatcacag gtgcttttgc ttttgcttta cctgtttttt ccaagacaaa
    15421 gttttttaca cgatccatat tggttaccaa gacgatcatg gatgtttctt tgcctgctgc
    15481 tttgatcgct tctaagtcag cttctgctac aagcgtatca ggtgtcactt gctgcccttc
    15541 tgttactttc acatcaaatg gcgtaccgcc taagtcaact gtgttgattc ccatatgaag
    15601 caagatttct aaaccatttg gcgttgtgat cccaatagcg tgtttcgttg ggaagacaga
    15661 gctgactacc ccagtaactg gagaatagat atgtccgttt tctggttcta caaagtagcc
    15721 ttcacccatc atcttttgtg caaaaactgg atcacttgct ttatctaaag aaacaactgt
    15781 tccgtcagca actgatgaga ggatagtcac actttcagct gaagtttgag gtactacttt
    15841 ttcaggcact tcatttactg tggctgtttc agtcgctaat tcttcttttt ctaaacggtc
    15901 tttaggaata ccgaagaaga atgtagcaac gaagccacca gcataagctg ccaataatcc
    15961 aaggatgtag cctaaccatt taccgtcaga gatcaatggg atcaaagcgg ctcctgaagg
    16021 tccaatagcg atagccccga catttccgat cattccgatc actgccccgc cgatacctcc
    16081 accgatacaa gcagtaataa atgggcgtcc tagaggtaat gttactccgt aaatcaatgg
    16141 ttcgccgatt cctaggatcc cgactggtaa agctcctttg atcatttctg ttaattcttt
    16201 atcttttttg cagcgaatcc ataatgccaa tgctgcaccc acttgtcctg caccagccat
    16261 cgcaagaatt ggaagcaata gcgtacgacc agtttcatca atcatttgca catggattgg
    16321 agtcaaaatt tgatgcagtc cgaacataac catcggcaag aataatgtgc ctagaacgaa
    16381 gccggcaaat gctccaccaa cgccaagtac ccagttgatc ccaccgacca tgccatcaga
    16441 aatgaaacct gctaaaggca tgatgaagaa aatttcaatc agaccgatgg caatcaatga
    16501 aagagttggt gtaacgatga tatctaccgc atcaggtact actttatgca gttttttctc
    16561 aacgattgac aatagccaaa cagcgaagat gacacctagg atcccacctt gtccagctgc
    16621 aagagcttca ccagtaaata agtttttcag tggtgcttcc ggattcatcc ctgtcagcat
    16681 cacaacggcc ccgataactc caccaagagt tggtgttgca ccaaaaactt gtgcagcatt
    16741 gatacctgta tagataacta ggtagctaaa catcccgttt ttcaagatgt tcatcacatc
    16801 gatatattgt tgccatgttg ctgcatcaag tgttccggct gtgactaagt ttgctaaaac
    16861 tgcggcaatc cctgccacga tcccagtacc gacaaatgca ggaatcattg gcacaaagat
    16921 attagaaatg tcttttaata ccgatttgat tttagatggt ttttgtttcg atttttgtgc
    16981 cgctttcatt tcttgtgccc gttcgtttac acggtctttt ccagatgctg atgaatttgc
    17041 tgtagctgta cttacagaag aaagtttttc tcctaatttc acgcctgcca aatcaaccat
    17101 ttgctgtgct actttgttca ctttcccagg tccgatgatg acttgaagct gttcatcttc
    17161 tactacacca agcactcctg gaatcgcttt taattcagag acatttacaa gatcatcatt
    17221 gatgatcttc atacgcacac gagtcatgca gtggacaatg ctttctacat tgcccatgcc
    17281 gcctgcccct ttataaattt gttttgccag ttcttccaaa gtcagttcgt tttttgccat
    17341 ggtcttttcc ccctcataag gtccctttta caaatccgtt tgcttcagct aattttgctt
    17401 gagcttcctc tttatcacta tttgtcaaaa tcatgacgat agccagctta acattttgat
    17461 ctgcttcttc aaagtacttt tcagctgttt cgtaatcagc ttctgtcgct tgcatgatga
    17521 ttcgttttga gcgttcgatt agtttttcat ttgttggttt gacatcgacc atcaagttat
    17581 tgaatacttt acctatacca atcatactga tagtagaaat catattcaag ataagttttt
    17641 gtgctgttcc tgacttcaat cgagtagagc ctgtcaaaaa ttctggacct gcatcgactt
    17701 cgattgggaa atccgcgtgt ttactgattt cggcttgttt attgcaagaa atcgttcctg
    17761 tgacagcacc gatcgatttt gcgtaatcta atccaccgat cacatatggt gtacgaccgc
    17821 tagctgcgat accaataact atatcatttt ttgtcaaatt cagcttgatt agatcttctt
    17881 gccctagctc ataagaatct tcggcccctt cgacagctac agtcattgct tcttctccac
    17941 cagcaattag acctatgacc atttccggtt ctacaccgaa tgtcggtacg cattcagcag
    18001 catctaacac acctagtctt ccacttgttc cggctcccat ataaatcaga cgtccacctt
    18061 ttttaaagga ctcaatcgtg ttagcaatca ctggttcgat acttgtcagt tctttttcaa
    18121 ccgcagcggc cactttctga tcttctttgt tcattagttg aacggcttga gaaatactca
    18181 tctcatctaa tccaaaagtt tcttggtttc tgcgttccgt tgttaggttc tctaaattga
    18241 ttttgctcat ttttatcttc cttcttttct tataaattga tagttcatac cagctttgat
    18301 ttggttgatc aatggtagct cttctttgat aactttagct actacattca ctttttcatc
    18361 cgctggcaaa tcatatttgg ttaattggat ctcacccata tagcgcaaat acttttcgtt
    18421 atctaaagta atagaccctt tcatacgcgt agctgtattt cttgctggga taggcggaat
    18481 ctctcgaaaa cgtgcatcta cgcttcgtat gacatctctt gcatcgtctt gccgattcgt
    18541 atgcttccct aatatatatt cgtaaaattc ttcatctaca ggttccgtat ggagcaagac
    18601 tgtctgttct ttttggaaag aagcaaattg ctcctgaacg ttttctgaca gacctgcatc
    18661 tccgatgtaa acaatatccg tgttacttgc taatagatct agagcgcctg caagaggatg
    18721 gatccctcga tgttcttcca aagttggcaa tccttgatat agtgggcctc tcaactctgt
    18781 atctcctggt acaaaacctt ggacaaggag accttgaatt ttgagccatt gatttttttt
    18841 ctgataccat tctttgtcca gccctgtttc gggacgtgga taataattat gccaagcttc
    18901 aatctgtgag aaatcagctt ctgcttcttt caattcatcg atatctttag gcgtgatcgt
    18961 gctagcattc aaactgattt tcaattgatg tgaccagtcg gcaatctgct gattggaaat
    19021 atgataatcc attcttaaac ctgttacacc aattgctttc aaaggcgtta gatcttctaa
    19081 tgaaaaaccc gctcgttcca atgcctctcc tgaaatatcg accatcaatt ctagtttatt
    19141 ctttttcgct tcgcttccta gttcgatcaa tcgcttttga tatgccgaaa catcatcttc
    19201 tggtatatgc atggaagtga aaataccgac aaagccattg gatgccattt ggcggatata
    19261 ctgtttcttc tcttctgaca aagaatcgtt cataaatatt gaaaagccaa acatattatc
    19321 cctctttttc tgtatccgtc ttcatttcta tgactagagt atatatctaa agaaacaaaa
    19381 tttcaatact tttattaaaa taattaaatt tcatttctaa aggttgagtt tatattatca
    19441 aaagaattgt cataacctaa aaaaggcact gctaagcaaa agcagtgcct tgaaatattt
    19501 tatttaaata acacaaaaac ataattactc ataagaaacc aattctgtat tatctaccat
    19561 gaatgtaaag aacccctcat taggcatcgg atgaaaataa gcattgatca ctcttatcaa
    19621 ggcattatgg caaactaaca gatagacatt ttcttcgtct tcgatgcatt ctttcaataa
    19681 aggaacgatc cgtgcataca catctaagac tgactcccca tttgggaatc gtacagcgaa
    19741 cgctaaacgt gctttttgaa agttctcatc tttgcttggc agtccatcat aatccccaaa
    19801 atccatttct accaatcgtt catcaacggt cagcggcaag ctgagacgat cagcaactgc
    19861 ttgtgctgta tctcttgctc gttgaagagg ggaatggatg attttcgtga ttggaacagg
    19921 caaatctact accttttctg ctagtttctg tgcctgcatc tgtccttttt cagtcagcgg
    19981 cacatctgcc cgtccgcaaa ctaatccttg tgcgttccaa gttgtttctc cgtgtctcgt
    20041 tacataaagc atgtgattct ctccttcgtt ggcaaagttc cccttaacca taccttgttg
    20101 ttcttgagat tacaagggtt agattctctc aagagaaata atctcagtag atactttatc
    20161 caggaaggtt tgatcatgtt ccactagtaa catggcaggt ttcacgttca agatcagttg
    20221 ttctaattgt tcttgattga agacatccaa ataattcaat ggttcatccc atgtatatag
    20281 ttcagctggc tgtgataaag atttagccaa ttccactttt ttccgttggc ccatactcat
    20341 ctgctcgatc ttgttatgaa aaacatctct ttccatccca agctttcgga ggttgttcaa
    20401 aaatgcttgg tagtctactt ggtttttctc tgcaaattcc gccaacgttc ctcgattgtc
    20461 ttcataattt tggcttgcat aactaatgct cagatgtttt ggctggtatt tttctcctat
    20521 aaccttgccg ttgaatgccc ccagaagata atggataatg gatgactttc ctgcaccgtt
    20581 aggacctgaa atcgccaccc gctgatgagg ctcgattgta aaatgaattg gctcgaataa
    20641 cagattttca taccctaatt gaagatcttc tacgcttaaa agtcgcttat ggtgagacgc
    20701 ttggctattc atcgtcaaag aatcgatata ttcgatatct tttaatagtt tttctttctc
    20761 gctgatctgg gtctccatcc gattcacgat tgctttggat cgtttcatcg tccgtgcagc
    20821 atcagcaccc actgctcctt tattcactcg tctagattca gtatcgatga atccgacttg
    20881 tttctttgtt ttatctcctt ctcgggaacg agaccattcg gctttttcag ctgctgtttt
    20941 ctttagccta ctgacttctt ttttcaattt ttcattttga gccatttcaa attcatcacg
    21001 aagttttttc tgttcttcat agatagagaa attcccttga taaagttcca gttgactttt
    21061 ttcgattgct aaaacatggt ccactacttc atcgataaat ccccggtcat ggctgaccac
    21121 gatgaagcct tgttttttct ttttcaaata agccgctact tgttttctac cagagatatc
    21181 caaatgattc gttggttcat cgattaacgg gaaatgagtg tcatccacaa ataaaagtgc
    21241 cagtaggact tttgtcttct ctccccccga tagtgtgctg aattctctcc ataagatttc
    21301 aggatctgtt tgcatcaatt ggagctctct ttcgatttcc catatctcaa aatccgtaat
    21361 atcatttaac acgtaatagg ttaaacgttc tttatctttt gtctgttgcg ggaaataggc
    21421 aaattcttgc tgatggatta cttgcccttg ataaggtagt ttgttttgta gaatattcag
    21481 taaagttgtc tttcctcgac cgtttcgtcc gataagtcct agtttccatt gtgtgtcaaa
    21541 atttagattt gcttgttcaa ataataatgt gccttggctg tcgtagccga atgtcagatt
    21601 ttttatttcg attttagaca ttttccatca cctcgtaaaa aaagaccagt cctattttca
    21661 gaactggcta aagaggatgc aagggttgga tacacacaaa aagacccata catcgaaaaa
    21721 accaagccaa tcccgaaaat ctgcacactg cttccttgtc tccacaaggt acagtctgaa
    21781 tgcagctttc atcaatcgac ttaatttttc aatgcacgga tccctcactt tgtcctatat
    21841 ttgaaattac gatagcataa agaaaatgat tttgtcaact acttcgggag gatggtgaaa
    21901 aatctgctaa gtgtctgtcc taagattttt gttttttcta atgtttgcct gtgttccatt
    21961 gcttcttcta aacctattgg acccaaatga atacttaaga caattgggag aaaatcagaa
    22021 agtccttcta gctcttggtc tctacttccg caaatagcga ttattggttt tcgatatttt
    22081 tttgcaagca ctgctacacc gtatggaact tttccgtgga tcgtttgctg atcgatccgc
    22141 ccttctcctg taatgatgac atctgcttgt tcgatctttt tttccaatcc agtgatttgg
    22201 ctgactaaat cgaaaccaga aaccatcttc gctcccaaca aaaaaagggc tccaccaata
    22261 ccgcctgctg ctccacttcc tggaatatca tctaaatcca cctcccagaa atcttttatt
    22321 tgctgagcaa tctttcctgc ttgtttatct aagaaagcta gcgtcgtatc tattcctcct
    22381 ttttgtcttc caaattgcca agcagctcct tgttgacctg tatatggatt ggtaacatct
    22441 gcagcgataa tcaattttac tttagaaaca agaacgtttc ttcgatcaaa atgaagtttt
    22501 ttagtagata ataaaggatt tccttctgtg tacccttcta aagaagcacc caaggattgg
    22561 agtaatccta gaccaccatc gctacacccg cttcctccta aagtgacaat aatttgttct
    22621 acttcttgaa gcagtgcatc tttaataagt tctcctaagg cataactgga agcaagtcgg
    22681 atagtttgat ccgttggttt atcaagtaga tctaacccca atgcttgtgc agattcgatg
    22741 actgcggtgt tttttccttg ccaagttgtc aataaatagc tgatttttcg ctgacggaat
    22801 aagagatcca tcccatcaat ggtacgccat gttcccctga gcgcgtaata gattgcttct
    22861 gcagtatttt ctccgccatc tgcaatcggc acattgattt ttcttccttt acgatcttcc
    22921 cagccattta gaactgcctg atttagttca aatgaagaag cacttccttt aaatgaatca
    22981 attgccgcca aaatatccat ctgtatccct cactttcttc ttaagtaaga cacttttttt
    23041 catttttttc aaacaaaaca atgctatttt cttccatttt tctatatact aaaagggaac
    23101 tcattgaata aaggagagaa aaaacatgaa aatcgcaata gctggggccg gagcaatggg
    23161 cagccgaatc ggattgatgc tacatcaaag cggaaatgag gtcctcttga tcgatcgttg
    23221 gcctgctcac attgaagcca ttcgtacaaa tgggttgatt gctgacttta acggtaaaga
    23281 agtggtcgca aaactgccaa tctattcacc agaagaaatc atcgaaagca acgaacacgt
    23341 ggacttgatc gtagcattaa caaaagccaa tcaattagat gatatgtttt gttcgatcca
    23401 atccattatt actgacaata cttatgtttt gtgtttacta aatggattag gtcatgaaga
    23461 tgtactggaa aaatatgtgc caaagaaaaa tatcttattt gggatcacga tgtggacagc
    23521 tggtctagct ggtcccggaa aagtcacatt gttaggtgat ggagaaatcg aactagagaa
    23581 cttggaacca gaaggcgaag catttacaaa aaaagttgta gaagtctttc aagaagcaaa
    23641 tttaaatccg atctatagcc ataacgttcg ttattccatt tggcgaaaag cttgtgtaaa
    23701 tggaacattg aatggtttat gcacgatttt ggattgtaat attgctgaac taggcgcgca
    23761 aaaagcggca gagagcatgg ttcgaacaat cgtatctgaa tttgcttcta tcgcagcaaa
    23821 agaaggaatc atactagatc aagaagaagt gtaccaacat attgcttcta cttatgatcc
    23881 tgacaacatc gggttgcatt acccatctat gtaccaagac ctgatcaaaa accatcgttt
    23941 gactgaaatc gactacatca atggtgccat ttggcgaaaa ggacaaaaat atgatattgc
    24001 tacaccatat tgtgcctttt tgacccaatt agtccatgca aaagaaggaa tccttggcgc
    24061 agaataaaag gctctctgat gagaatcagc tgaatcagca gctggttctc tttttttaag
    24121 cccaataaaa gatcaaaaag aaaacacaac tctcatgatg ccataagaat tgtgcttgtt
    24181 tgtttcagta cataatctct tcaaaatgta ggttttttat ttgttttcaa cagaggagag
    24241 gtcatattcg tattgctcat aaatagtgga atcgttagtg ataaatcgtt ttgcccaatt
    24301 ttctgcatca taaaattcca atagctctga taggattggc ttaaatggtg tatgcagaaa
    24361 ttccggtaat tcatctaaat cacgttctcg aagcaaaaaa atattttcag gacgataaaa
    24421 atgataatga gcaatcgctt gattatccgt cactagtttc ttttgaaaat acatggattc
    24481 aaatgtccgc aaagtcactc cttgctgacc ctcccgcaac agttccagaa tcccgcgcga
    24541 ttcccctgtt tttttcaggt actcttcata agtaagatat ccaggttgtt cattccctcg
    24601 atgtggaaga atgaaatagt tggtcgtaac atctcttttt ataaattcct gcttatagct
    24661 ttctgctcgt tcttttctcc cattattcgt tgcaccaaaa aatagatccg tctttggttc
    24721 gctagaagaa aaattcatat gtttcgaata aaatgaacta ttatgtttca gcccaagttc
    24781 ttttgcttcc aaaaaatcaa aatgataaat ctcatcgatt gccggttcat cttgtgcctg
    24841 cttcagcatc gcctcgtgtt ctgctttgaa gtgattccaa aaaaataaca caatttttgc
    24901 attggtaaat gggcgcacgc gtcgagccat ctccatatca aatccataat ctaaaaaaac
    24961 gatcagctga taatttgcca actgttgtct agctgattta atgaattccg actgtagtaa
    25021 atcttttccg gaccaaacat ctaaagagcg gatggaaggc aaaaaataaa gtgatttatt
    25081 ttttgcaact agcagtacgt catttttgtt catcttttcc gcctcacttt ctctgtcact
    25141 ttttcctatg atagcctgtt taaatagtaa aaagccagta aaacgtttcc tatatttgta
    25201 tgaaaattta acatttgtat gaaaatatga tgaaataaat cattcttcta ttaaaattcg
    25261 cctaaaagtt ttctgaatag ttgtaattgt ctgaaaattt gttatcatta gtcattgtta
    25321 aatattgcta tttattctta atataaattt tgattgtgaa tttaaaagaa agaggtcatt
    25381 tatggaagat aaaaaattga aaaaacaact tagctccaga catatcacca tgttagcttt
    25441 aggtggagca atcggtgcag gtctatttaa aggaagtggc gaagcgattg ggatcgccgg
    25501 cccttctgta ttgattgctt ttttgattgg tgggctgatt ttatatatcg tgatgaaagg
    25561 tctgggaaaa atcgttttaa gcggtggcga cacgcatcac ggattatctg gtttgatacg
    25621 cccttatcta ggtgctcatt ctgctgattt tactgattgg gtctactggt ctatgtggat
    25681 tatcaatatc atcgctgaag ctgttgcagc tgcttccttt ttgcagttgt ggttcccgca
    25741 cattccagcc tgggtatttg tttttatgtt ggctgtttta actacgatca tcaatcttta
    25801 ttcggtacgg ttattcgcag aaacagaata ttggctggct tttgctaaaa tcagtgtcat
    25861 tatcttactg atcatttttg ctacttatct tgtcggtcag caaatgttgg gcacaggcgt
    25921 tttccctacc cttcagcaat tgactgacca tggtgggttt actcctcatg gtatgaaggg
    25981 aatcatcagt tctttgcttg tagttatcta ttcttacggc ggttctgaac tgattgctat
    26041 cacggtaagc gaagcagatg atcctaaacg tgcgattcct aaagcaatca aaggtgtcat
    26101 gggaagaatc atttcctttt acatcattcc tttgtttttg cttttgattc tttttccttg
    26161 gaatacttta gcaggcacga atgtcagtcc ttttgtgatg gtatttgaaa aaatgaatat
    26221 cccatttgca gcggatatcg tcaattttgt gattgtcctt gctttgttct catctatcaa
    26281 ttcgggtgtg tatgcatctt caagaatttt gttcttccgt ttgaaggatc gaaaaggttc
    26341 ctctagaaaa ctagctgttt taaacaagca tcaagtaccg caacgtgctg ttttattttg
    26401 taccagtgtt ctttatcttg gtgtagctct ttcttatttt gtcggtgaca aattatttgg
    26461 ctatctggca ggttcccttt cgtatacagt attattgatc tggatcatta tcagtgcggc
    26521 atcctttgta ctggcactaa gaaaaggaac attatgggat aaaggaatca gcttttttgc
    26581 attagctgtc ttaggattga ttttccttgg tatcctgttc acgaactcca ttggggtaac
    26641 tgtattgact ggcctgcttt acctgttcat ttatttcagt tatcaaaaaa agaatgatgc
    26701 gttcgtattg acgaatgaat gaaatcgata gaaaatttta ttttatttat gtggaataga
    26761 tggagctatt gaagaaagga gttgtgacaa aagtcttgtt gcaactcctt tttccgaata
    26821 aacagtctca tgacctcttg gtaaacggtg ttcaaaaacc ctctgtgcaa aataattgat
    26881 tgttctccat gctcatgaca tgatagaaga aaggaatgaa aaaaaggagt ttgttttaaa
    26941 tgaaaaagta ttggaagttt ttgttgatga tcggtgtttc aacggtagtt atgtttggta
    27001 tgatgtactt gaatgtctat gcgctcgatc atatcttctt cagtcgtaca cgagtattca
    27061 tggcgttgat gatgggtgca gtcatggcag tcatcatgct gttgttcatg tggaaaatgt
    27121 atgaaaacaa aaagataaat gttatgatct tagccgctag tcttcttgtt tttgccggtt
    27181 ccttgtttat ggtacgaagc caaactactg tcaacgacac agcttggatg aaggccatga
    27241 ttcctcatca ttctatcgct atcctggcaa gtaaacgagc tgatttaaaa gatcctgaag
    27301 taaaaaaatt agcggaagaa atcattcagg cacaagaaga agagatcgac aaaatgaaaa
    27361 aaatgatcaa agaaatggaa tgaccattat ccgtccaaat aaaaaaagag gatgttccag
    27421 aagttatcag cttcaagaaa taagaagaag ctacttctgg aacgccgtga ataaggggtt
    27481 gtgaaaaagc tgccctgcat caagtaataa gaacagccaa acaaaaatag cttctcctat
    27541 ttttcgtttg gttaggctta ttaccgcaga tgcaagcttt tgaacaccgt ttattaagag
    27601 gttgtaaaat cagcgttctg acctgagtat tagagagaca aatggaaaaa tagttcctca
    27661 tattttgcca ttttttgagc taataccgag ggtcagctga ttgaacaccg tttatcgaga
    27721 ggttgtgaaa aagtcgctta gtttcgcgta ttaagaaaaa ttttgtgaaa tggctttcca
    27781 catttttgta taaatttggc ttaataccga gaaactggct tttgaatacc gtttattcgt
    27841 aaaagggatt gtgacaaaag tcttgtcaca atcccttttt atgtttttat tgttctaatt
    27901 gcgctgtctt gcttactgct tcttgcggtt tcacaaattt ttttgagatt ccccaacctg
    27961 tgaaggcact gaccatcgaa aacacaggag aaagcaaact gaaaaatacg aatggcaagt
    28021 aagtgagtgt aggaatacca aatgtacttg ctacgaaact tccggcaatt ccccaaggca
    28081 ctaagtagtt gattactgtt ccaccatctt ctagcgttct acctaataca gttggatcaa
    28141 atcctgcttt ttcataagtt tctttaaatg cattaccagg cagaataaca gataggaatt
    28201 gttcaccaat aaaaatattt actccgatac ttgtcatcaa tgttgtaaaa atcaaatttg
    28261 gtgtgttcgt catctttctt gatactcgtt ccatcacggt tgagatcaag ccaaattcga
    28321 tcaacaatcc acctaatgac aaagttagaa taatcaatga cacagtaggc atcatacttt
    28381 cgattcctcc gcggctaagc agtgaatcga tttgttggtt tccagtattt gaaacaaacc
    28441 ctgattcgat gaccccagcc aacttcgtta aggaagtttg tgggttttga ataaaaatca
    28501 ttcctactgc tacagcgata ttcaacaaaa tcgttaaaat tgccggcacc tttttccatg
    28561 cacacagtaa cattaaaaat aaaggaatca gtgaccagat tgaaatagta aagtttgctt
    28621 ctagaacatc agtcacttca cgaatagatg aaagacttgc ggctttactt gtgtgtccta
    28681 aaatcatgaa taaaaagaaa gaaacaacaa atgccgggac agttgaccac atcatatgct
    28741 tgatgtgttt gaacaaatcg gcatcaacaa tagctgctgc tagatttgtt gattcggata
    28801 atggagacat cttatctcca aaaacggcac ctgaaacaat agctccaaca accagcgctg
    28861 gatttaagcc tagtgttgtc ccaataccga aaaatgcaat cccaatcgtt gacataacag
    28921 taaaggcact ccctacagcg ctacctacga ttgcacacac aacaaataca gaagggacaa
    28981 accactggac actgatcaat ttgaacccaa tgaccattag agttggaatg atccctgctt
    29041 gtatccatac agcaatcaat gaaccgacta aaataaaaat aaagattggg atgattcctg
    29101 gtttgatgcc attgatgatt ccttcgtgca gccaatctgt cgggtatctt tttatcactg
    29161 catacaacat gaccattccg atcgctgcta agatcgtgat attaggtgag acctttaatc
    29221 cgacaacgcc aatcgcaatg cctaaaacaa taaccattaa tacaagtgtt gcttctaaaa
    29281 aacttggtgt tccttttttt cccatgataa tctctccttt tttagttgtt cttatgttct
    29341 aaaaaagtta agatatcgga ttactccgcc gcaattcatc cgcgattcct cctttataaa
    29401 aataaaaaga cctgttttaa actaaggacg aatgactccg cggtaccacc ttaattaaaa
    29461 acagatctgt ttttcacttt caaactttta ttcaaatgta tggactttcc atgtaattcg
    29521 tcaattaggc ttgatcggtt cacagcgacc accgactttc tgaacaaaac acctaaccgc
    29581 tactgcgtga aaagttgact tgtacgaagt ttaccttcca taagatcatt tgtcaatgaa
    29641 aaagaaaata ttttttcatt tttctgaaaa ttatttttat aactttcaca cacaagttta
    29701 ttttttacta gaaagtagcg ccctcttgtt tttttcttgc tactatttct tgatgttctt
    29761 gcttgattct ttctaatagc tttggctctt tcaacagatc aagagctgta ttggcgagta
    29821 gttctgctgc aatcgcaata gaagcaaggc ctttgtcact tttagcagca gctttgaatt
    29881 cttctgtgtg tccggcaata tatgtctcag agattgctac cgtaggctgg attgttggta
    29941 tgacttggct gacatttcct acatcagaag aaccgagact tgttctttgt tccgtttcta
    30001 tttcttcatc tggaacacct gcttcttttg catgctggaa gaataagtca tcaaaagaag
    30061 gagtaacgat gacatcatca actgcatttt ggaacaatcc aaactcaaaa gtcgtgcccg
    30121 tacttatcgc agcacctcgt acgatatttt ccactttttc atatacttta tctaatgttt
    30181 gccgatttcc tgcacgtaaa tagaaacgtc cagcagcgta atctggaatg acgttggcag
    30241 ctactcctcc gtctgtaatg atcccatgga tattgacatc ttttggcaat tgcaagcgta
    30301 gagcgttgat tccattaaac acttggatca atgcttccaa agcatttact cctttttcag
    30361 gtgctgctgc tgcatgggaa gcgacaccgt aaaatttgat gtcgactgga tcattcgcta
    30421 aagattctgt cgttttccca taacgaaaag ctggatggac gcataacgct acatcgacat
    30481 cctcaaaaaa accttctcga acaaagctgc ctttagctga tccgttctcg cccccttctt
    30541 cccctggggt gccatataca cggatttctc caccagtttc atcaatgaat tgcttcacta
    30601 tgctggcggc taatacggag taagtaccaa acaaattatg cccgcatgca tgaccaatac
    30661 ctggtaaagc atcgaattct gccaaaaagg caattgtggg accgggttta ttggaaatat
    30721 aacgtgcatc aaaggctgtt cggtgtccag ctacttcttt ttctacccaa aacctttctt
    30781 tttctaattg tttaatcaac gctgctgagg aataaaactc atagttactt acttcaggat
    30841 gttcgtggat atctagagca agtgcttgat attctggtaa acgatattgg attagatcct
    30901 tcaattggtt tgttgagatt gttgttgtca taaaaaattc ctcctatgct agtcattttt
    30961 cttattttct cccgaatgtt tcccatgcag gcacgcttga tccttttgat gttttctcga
    31021 ttactttgac tgtgtcttct gtttgataag cgtctactat tttttgatac gttttattgt
    31081 tttcatcttc ttttcttgca acgatgatat tcacataagg acgtgcatgg tcatccactg
    31141 gttctaagaa gatggcatca gaagcaggat taaatccagc atctacagct acaccgctat
    31201 tgatcactga aatgtctaca tctgacaaag cacgtgctgt ttgggaagca tctaattcag
    31261 aaatgtctag attttttcta ttctcggtaa tatcagaaac agtgggcgct tgtccttttg
    31321 ccttatccac tttgatcaat ccagcagttt gcaataacag aagagctctg ccgccatttg
    31381 taacatcatt agggattgct acttttgctc cgtctttgat atcagctaca tccttcaaag
    31441 tgttagaata aattcccagc ggtgcattga cagtatttcc gatcgatact aaatcagttc
    31501 cgtgttcttt attgtaattg tctaaaaaga attgatgttg gaaagaattc aaatcaatcg
    31561 atccatctgc taaagccaca tttggttgat tgtaatctgt aaatttcacg tattccaaat
    31621 taatgttctc ctttttcaaa cggtctttca cgttgtccca tacatctgta tcttctccaa
    31681 taatccctag ttttacgata gtttcttttt tgtcagttgt atctgctttt ccttggctac
    31741 accctgccaa ccaaataatt gctacaaatg taatgactac aaaacgaata atttttttca
    31801 ttttttctcc tccactttca atgtgtaatt tttttaatca gttgattact gataaattga
    31861 cttaaaaaaa ctaagattag aatcaataat gtagccacaa tcgttacatc tgtttggaat
    31921 cgattatacc ctctcgatat agcaagattc cctaatccgc ctgcacctac tgctccagcc
    31981 atggtagtca gtccgatcac gttgatgata gtcaacgccg agacccgaat gataccaggc
    32041 aaaccttcta acaaatacac acgtaaaatg ataccgattg gactggttcc catcgcttct
    32101 gccgcttcga tcaccccagg atcaacttct agcagtgcgt tctcgatttg acgagcaaaa
    32161 aacggaacaa ctccaaaaat caatggcact aaagctgctt tcgcaccaat cgtcgttcca
    32221 actaagaaac gagtagtcaa tgctaaaaga gaaagcaaga taatgaaagg gatggaacga
    32281 atgatattga tcagcttatc aagtatttca aatagcagac ggttagctag taatccatta
    32341 gggcgtgtca ctactaacgt cacacctaag atgattccaa tcatcccgcc aatgaatgct
    32401 gtccaaaaag acatatatat tgtttgcttc gtcgcttcga caaattcatc cggaatcaaa
    32461 taaacattgg gcaaatatgt tttaaagaaa tcagtcattt tatcttcctc cttcgatcac
    32521 tttgatacga ctatctaaat cgtttttttg taaaatattt atttttacac cagatttgat
    32581 caaataatcc aatgcttttt tccgctgttt ggtttctcca gctaaagtaa cgatcaaact
    32641 ccctaacggc gtttcttgta ataactctac attgccatat aaaatattcg ctgttacttg
    32701 atatttgctg tacagatgag cgattagagg ttcgttcgtc tgatttccaa tataacttaa
    32761 ttccaccagc catttattgg cgatctgatc ggcaaaagca gaatgggcaa tgatcgtttc
    32821 taatgcttgg tcaagatgag tagctgttcg aataaaatct ttggtcaatt cttcttcagg
    32881 attgctgaag atctggatgc tgctaccttt ttcaacgatt cttccatctt ccataaccgc
    32941 taccttgttg cagatttctt tgacaacttg catctcatga gtgatcagga cgattgttaa
    33001 acctaactgg cgattcaatt tttttaacaa tgcaaggatc tgaagggtcg ttttcggatc
    33061 aagtgcactg gttgcttcat cacataataa taccttaggt tcattcgcca aagctcgtgc
    33121 gattgctact cgttgttttt gtccccccga cagttgttta ggataagcag atgctttttc
    33181 ttccaaaccg acaagttcca gtagttcatt cactttttga cgacgttcct gtttcgattt
    33241 tccagaatac tttaaagaaa aatcaacgtt gtcaaaaata gttcggcttt ccattagatt
    33301 gaaatgctga aagatcatac cgatggcctt tctttctttt cgcaattctt tgggagagag
    33361 ctgactcaga ttcgtctgat tgacaatgac ttctcctgct gttggtttct gcagcaaatt
    33421 catcacacga actaacgtac ttttccctgc acctgagtaa ccgacgattc cataaacatc
    33481 gcctttttca atcgttaaat ctacagaatc tactgcctgt atctcttttt ctttttgttt
    33541 gaaacataca gaaacatctc gcaattcaat cattaaaatt cctccttatc taaaaaaagc
    33601 gtcctcaaga aaatgaaaat gtcattttct tgaggacgct tttttgcgtg ttaccacctc
    33661 atgttcaagc tgtgttcaca cacaactcct cttcaagtac gatcatactc tagcaagata
    33721 tcggtcgcag ccgtcatcgc cttacatcta tgctcgggat gaattgctcg atggccattt
    33781 tccaatagtc gttccttgct tcttctcagc taccgaagct ctctgtcaaa ggttcttcta
    33841 tcgtacttac catttcaatg caaataatat tcaaaaaaaa agtcctttta ctaacataaa
    33901 ctatgtcagt aaaaggacga cgtatcgcgg taccaccttt tttcggaagt ttccttccct
    33961 caagcccaac atctcaaaaa gagagattgg cgttcgtttt aacggtcgaa cagttccgaa
    34021 tttgcctact tatcgacaaa ttattctcca aggccatttt cagcttttct tacttgactc
    34081 ttctcaccag ccagagcctc tctgcaaagt ttccaaactt actttccttt tcaacgaaat
    34141 cgatattttg ttgttgggat taatctacat gaggaaaact gaaaagtcaa gtacgaaatt
    34201 caaattattt tttctatatt tatttttctg tttttttatt taacatttca ataacagttc
    34261 gaagatcttt tccttccgca agaagtttca atttttcctt ttcttcgacg gtataatttc
    34321 ggacaaaact ggaatcaggt atcaaaacaa cgcctaatcc tgctcgtgaa gcagctgttg
    34381 ctcctactaa agaatcttcc actgcaaagg ctgaactagc ttctaatcct gttcgttcca
    34441 aagctgtcag ataaggaatc ggtgaaggct tgttctccga cacttcgtct ccgaatgtaa
    34501 acgtgtccaa gtaatcagct aatttaaagt gatccaagat atctgttgct cttttcgtaa
    34561 cagtggtcgt agcaatgcct agtttcatat gacgttcttt taagtctgcc aatatttctt
    34621 ttgcatatgg ttttaagtgg atggctccgc ttgtcagctg ttgataaaaa tattcttcac
    34681 gcttcgctcg gacttcctgt actttttcag ggctacctgc tgctttcact aaaatatcaa
    34741 aactttgttt ccagtttttt ccgccccaag cgtcaacgat ttcttttggg atatggatat
    34801 caaattgctg aaatccccaa agccagccat cacgatacgt tttttctgtg tcaactaaca
    34861 aaccatctaa atcaaatatt gcacctttca tctatacatt aatcctttcc aaaacaatac
    34921 acatagttta gcacgactgg tccaaaaaga acagaagcaa agaaccagcc ttctcgttta
    34981 taagagttct tggaatcgac ggatatagat tttcttcaca gctgtcacaa gcgccagata
    35041 agcgatgact gtagcaaata accataacca gtaattgcca ggaagtggca tcaagcctaa
    35101 ttcagcacca aaacttgtaa aaggaaggac agaaccgaca ataattccta atgtagtgat
    35161 aacaaacatg gcaaatgttg cgttgctttg gataaatggt actttcggtg tacgcagtgc
    35221 atggagcact aaggtttgtg tccatagtga ttcgacaaac catcctgcat ggaataatgc
    35281 gatgaaaatc actcgctgtg aactatccaa tgtatggaaa cttccgccca ctacagcagg
    35341 acaaataaca aaatacatca acaagtaagt agtaatatca aatatcgaac ttgttggacc
    35401 aaagtaaacc atgaattccc caatactaga tgcattccat ttttttggct cgtccaaata
    35461 ttctttatcc atccgatccc atggcaagga aatgcaggaa acatcataga tcagattcaa
    35521 aaacaaaagt tgtaaaggca gcattggtaa aaatggcagg aacgtacttg caatcagcac
    35581 ggaaaacata ttgccaaaat tagaacttgc tgtagccttg atatatttca tgatattccc
    35641 aaaagtttct cgtccagaaa gaaggcctct ttccaaaatc gtcaaatctt tttctaaaag
    35701 gatcacgtct gcagattctt tcgcaatatc cacagctgta tctacagaaa taccgatatc
    35761 agccactttc attgcaggag cgtcattgat accatctcct aaaaatccga cagtatgacc
    35821 atttttacgt aaagctgtcg tcaaacgtgc tttttgctga ggattcaatt ttacgaatac
    35881 ttcatggtct tcagctactt gtgctaattc atgatcactc atttccgaga ttttctcacc
    35941 ggtaaccaat tcattgatct ctagacctac ttgacggcac actgaacgtg taactaattc
    36001 gttgtcccca gtcagaactt ttacagctac accatgttct ttcagagctt tcaacgcttg
    36061 tttcgttgtt tcttttggtg gatcaaggaa agcaagataa ccaatcaaaa ccatgtctga
    36121 ctcatctttg actgaaaatt ctccaacgac gcttggattg gtcttttgtg cgacaccaat
    36181 cactcgtaag ccatcttcat taagtgactg tacttttgca agtacggctt cttttcgttc
    36241 ttttgttaat ggtgtaacag taccgccaag atcaataaat ttagaaatgt tcagcatttc
    36301 ttcaatcgcg ccttttgtga tcatttgtgt tttaccagct ggatcagcaa ccacaacact
    36361 catacgacga cgttcaaaat caaaaggaat ttcatccact ttttgataat tgatcttatc
    36421 tgtatctaaa gtttctgttg cttcatcaat gatagccaca tctaacaaat ttttcagccc
    36481 tgtttgatag tagctattta gatacgcatg gcgcaaaaca cgatcatctt catttccttc
    36541 acaatccata tgatattcaa gaatgatttt atcttgtgtc aatgtccccg ttttatccgt
    36601 acataacaca tcgattgcac caaagttctg gatggcattc aaatttttga tcactgttcc
    36661 ttttttggcc atcacacttg ctcctttgac caaattcgtc gtaacgatca ttggaagcat
    36721 ttcaggtgtt agaccaactg ctacagatag tccaaaaaga aaagcttcta gccaatcccc
    36781 ttttgtaagc ccattgatga cgataactgc tggagccatc aatgacataa atttgatcag
    36841 tagcatggat gttttatgga tgcctaattc aaagcttgat tgaattggtt tttcagatag
    36901 cgtttctgct acatgaccaa atagtgtttc atttccaaca gaaacaatga ttccttccgc
    36961 acttccactg atgacattac ttcccataaa gacaagattt tcgtaacttg tttcgctttg
    37021 ctgatcgtct tcacatactt tcgcatgctt ttctactgga tagctttccc ctgtcaatgc
    37081 tgcttgggaa atgaataaat cctttgcgga caataaacgc atatccgctg gtatcatatc
    37141 ccctgctgaa agccgaacaa gatctccaca gacgatttct tccataggaa tctctgtgta
    37201 ctttccatta cgttttacag ctgccgttac tttcacaagt gatttcagtt tttcagccgc
    37261 ttgattggaa cgtacggact gaatcaacgt cattgtcccg ctgacgataa ccatagtgaa
    37321 gataatggct acaccgaaaa gatctttatc tccaggagct gctaatacat attccgtgat
    37381 gaatgaaata attcctaatg cgatcaacac taatgtaaat ggcgtgatgt atgctttcaa
    37441 tatttctaca atgaatggtg tttttttgcc atatgaaatc gcattttcac cgtaattttc
    37501 tcttgaacgt tcgatttgtt cttctgtcaa tccatttgct gatgcatgga atagttcaaa
    37561 taattcttgt ttcgtttttc cagcaaaagc tgcatagatt ttttgtgtat ctttcttttt
    37621 ttccatctca atctctcctt tgtgtccaaa agacagctgt tctcgctgct tttggtcttt
    37681 agctattcat cggatcgata gagaaaaaac tgaaaggggc tgagaacaga tagataatag
    37741 ccctggcctt tcctctagat ccttgttttt cgaatgactg tcatccattt gttctcatct
    37801 cctttactaa gtaattttaa ttcatgactt ccaatcattt ttcgaatagt tatttttcca
    37861 attagaccac ctccttttta tttttcaccg atggggattt gcggcaacaa aaaaacctct
    37921 cgccaaatac tcagcgaaag gtttgaaaaa gcatattaaa aaaagcttct cagatctcat
    37981 cgttgagttt tgacactgta caacgtaaaa agcagatacc tgcttttagc taccttatga
    38041 aaagccttat ctcgacaatt cctgttctac ccattggcgt ctctcgacgt ttttgggcag
    38101 tagcctatgt ttgtatagga gcctcaccta acaaatctta ttcggttttc cttcgttaat
    38161 ataacaacta aaaaagagga tgtcaaatca attttccatg tttttcatac tttttttaaa
    38221 atacacacgt tatattcctg tctcacgcgg ttttatttgt ctcattttcc acctcccgct
    38281 ataatagaaa tatagtagga aacgaggtgc tgttatggaa agaacacttg ttattatcaa
    38341 gccagacggc gtgagacgcc atttagtcgg aagcatcatc caacgttttg aagcaaaggg
    38401 actggccatt gctgaaatga agtttgaaac tatgactcct gaattagcaa aagaacatta
    38461 tcagcacttg actgagcgca gtttttttga tgaactgatc gattacatga cttccggtcc
    38521 tgtcgtttat cttgtgctgg tcggcgagga agtgatcgat atcgtccgta agatggttgg
    38581 agcaacaaaa gctgccgatg ctgttccagg tacgatccga ggagactacg cgctgccggg
    38641 gactgaaaac atcattcacg cttctgattc ccgtgatgct gcggtaaaag agatcgcccg
    38701 ctttttcccg ccattcgata taaacaaacc gtctgaaaaa gcggcgaagt gatacaaatt
    38761 ctaaactgct ggtctagttg ctgggttcag caatggccag cagttttttc tctaaggacc
    38821 tccgttgatt ctttcctaac atatattcga gcactgtgaa gaattcttgg tagtctttcg
    38881 tcttttcttc ccctaaagca gcagaaatca atctttgata ataatctgcc atcaatgttt
    38941 catagatcac tgtacttctt tctttatgtg ctaagctccg tttcatatct tcattcacac
    39001 tacgtgcctt tttcaaccga ccattttcta tataaaacga agtcaggttc attaaaaaag
    39061 taaagagata ctgctcatga ttgttcaact gtgctaatcg tcgcattctt tttttgattt
    39121 tcaaataatt gttctcaatg aaccgatcac tgaatatcca aggaaaatca gctagaatag
    39181 atagttcaaa ttgaccatag tctggtatct gcatcaagta ttcttcaatg attcggcatt
    39241 cataacgtaa aaattcaaat tctgtctcag atacttctgt tttttcgacc atgtgaagat
    39301 tcaaaaccaa ataatacaga tcttctgttt tcttaaaatg cctacggtta ttgctgatct
    39361 ctttataagg atcaaagctg tcttcttgta cccgatctag cattcgtctt tttttcttta
    39421 actttattgg ttcatccagc tggtttcgca tccattcata ttctgcaagt gtcatgttca
    39481 ttcgctctaa aaatgatctc aatgttacaa atgacatttt tttcactgaa tttgtctcaa
    39541 tcccgactag agaagtctta aaacagattc cttccgctaa ttgggactgt gtcaacttct
    39601 tgttgactcg aagtttcttg atcaattcac catcatacat aatagttctc ctttttaatt
    39661 taaatttttc cacattcaat aaatagatac gttaaaaagg aaaaatacac gaaaaaaata
    39721 aaaaaatgtt tttttcataa aaaataccaa ccttcaagca ttagaatgtt gtctaactac
    39781 tgaaggttgg tttgattgtt tattgattat ttttttgctg ttcaacgtct gttcttgtta
    39841 ctggatataa gttggttgtt gttttattac cattttttga aaagacactt gtcgaatgtt
    39901 cgcctttttg cttttcgatt gcttcattac gttccgcttc atttttatag tcatattttt
    39961 tcggatcaat cgtctcaaat ccgtttggca cataaaaccg caagagattt tcctgattca
    40021 gcttatctga aagacttaac gcagtcttga ctttttcctg atctttggag atttcctcat
    40081 cgattcctgc tgctttcgta tcgatgattt ctcctgtctg attattgtaa gcttttcctc
    40141 ctaggacggt gtatttgggt gtcacaaagt tcccatttcg aaaagcaact acttgatcat
    40201 gctgagaaga taataagtct gtaccaaaat gaagataatc tttatcatcg attcccaata
    40261 gatgtagtaa tgtcggcaaa acatcgattt ctccaccgta ttcatgattg actgtccctt
    40321 tagtataacc aggcatatgg atcatcaatg gcactcgttg catctgtgcg ttgtcaaatt
    40381 catcccatgt atctggatct ttaccaagag cgcttgctaa atctttatta tctgtattcg
    40441 agatcccaaa atgatccccg taaatcacaa agattgaatt ttggtaaata ccagattttt
    40501 tcaaatacgt aaaaaattgt tccaatgatt gatccagata atgtgccgtt cgaacataat
    40561 tatctactgt gctattgcca gtattcaaag atggaaagtc gaaattcttg tcatccgtat
    40621 aataaggcgt gtggttggtt acggaaagaa atttcgtgta aaacggctgt tgtagatgct
    40681 caagatattg ggcagattct ctgaataaat ctttatctaa tatcccataa cctaacgttt
    40741 cgtcggattc atcaaaataa gatcgatcaa agaaattgtc ataacctaag tttttatata
    40801 catgatctct attccaaaaa ctagctacat tcccatggaa aacagcgctt gtatagcctt
    40861 gcttttgtcc aaggatcgct ggtgcggctt gaaagacatt gtctgaccca agttctgtga
    40921 ataatgagcc ttgaggtaaa ccaaatgtgc cagtttccaa catattttca gcatcacttg
    40981 tctttccctg tcctacttca tgaaagaaat tgtcaaagct gatcgttgct tgattctgga
    41041 aaatactatt taaaaacggt gtgacttctt gtccgtctac cttcatatta atcaaaaact
    41101 gctggaaact ttctaaatgg agaacgatca catttttccc tttagcgatc ccaaatgttt
    41161 cgggatttgg ctctgcataa tgtttctttg tataatctaa aacttcatca atcccattac
    41221 tgcttgcatg cgcgcgaaca ctgctagtca ttcctgtttt gattccgtca taaatagtat
    41281 acgcatcgat tcctagatat ttgacaatat atgaacggtc aaatgttcgc tgcagcaatt
    41341 gtggacggtt ggcttcgctc agcgctagat ttcctgaaaa aataagccct gaaagacagg
    41401 taacagcgat agccatcggt ttgccgacag ggttgctttt gataggtatt ttcttgacct
    41461 tcaaccaaac cagtagccca atgaacacag cgatatccag ccagtagatg acatcatggg
    41521 gcttcatcaa agaaaaggag cttcctgaca atccttgcga cactttcgaa aagcctaata
    41581 cagatttgac tgtaatgaaa tctgtgaatt cccgataaaa aataatattc aagtacaaca
    41641 aaagcgtatt taaaatatca atgatcaaaa gaatagctaa cgctggcttt ggctttttaa
    41701 tatacaaaga aaggccaaaa aacaataagg tagtcgcaat tgggttgatc cacaagataa
    41761 agtactggat cgtaccttcc acacctaatg aaaaatctgc atagtaggca atgatcgtct
    41821 tcagccagaa aaagaaaaca gctgtcaaaa cgattgctaa acgattttgg cacaattttt
    41881 taagttgtgt cacgtatatc acttctctct attcacattt tttcttattt tgctcctgaa
    41941 aaatgaaaat ttcctaaaaa agttgttatc ttaagatttt tttattcttt ctaaagatta
    42001 ttccgtccac ttttcacagc ataaaaccgc tgcgatgtaa gcgattaaac taatgaagag
    42061 ataaaagtct tgtggtgacc gcttcactcc acggtattca aaagccagtt tctcggtatt
    42121 cgtacaaaaa aagaggctgt gacaaaattc ttgtcgcagc tccttatttc ttgatgcagg
    42181 acagcttttt cacaacttct tccttaaacg gtgttctcct gccaactcct cggccacaag
    42241 tcacaatatt tataaaacat gagaagctgt tttttgaaat tgttccttgt ggctcagagt
    42301 taaacggcag gctcaagacc tcttgataca cggtgttcaa tcagctgacc ctcggtatta
    42361 gattatgtca ggacgctaat tttacaacct cttggtaaac ggtgttcaaa agcttgcatc
    42421 tgcggtaata agcccaacca aacgaaaaat ataaggagct attttttgaa attgctcctt
    42481 gtggctcaga gttaaacggc aggctcaaga cctcttgata cacggtgttc aatcagctga
    42541 ccttcggtat tagctcaaaa aacggcaaaa tatgaggaac tattttgccg tttgtttctc
    42601 taatactcag gtcaaaacgc tgatttcaca acctctgttt ttcattgtcc ataggattga
    42661 tattgtcctt gtgatgactc attcgtaata attgaggctt gattttcttc acttcccgtt
    42721 atcgtttgtc cactgccaga atctgaagga cttgttcctg tcgactcgtt tttttgagaa
    42781 gagacagttg ttttcttgac ttgttcattt gtctttttcc attccgctaa acgatcggcg
    42841 accacttttt tgtctacttt ttcagaataa gcggtgatca cttttccatt tttcaaacgg
    42901 gctactttgt cgccatcatg agtcaaaagc aaaacggtca aatgcttgtc tttaaattga
    42961 tattgtctga gagaaatcgc actaagtacg tcatcgatat taataaattg attttcttct
    43021 tttccttttg gcaaataaat gtccaattcg cctttttcgt aattggcagt tattttatac
    43081 tccgcccacc aataagttga gaattctttt ttgaaattat ctactgattc tttgcttgct
    43141 acgatttttt ctgtttcttt ctgagcaaga tctgctgtca tttttcctga aaatgctgcg
    43201 gataaaataa gcagtaagaa aaaaccgaat aaccaaaaga gaagaaacgg tctttttttc
    43261 ctcatgcttt cctccactcc tatttaattt tgactattct attatataca aaaaatgaaa
    43321 tagaaagaca aaaaagttga aaaagatcgt aaataattga acttcttttt cacaaaatgg
    43381 taagaatgga gaatttcacg catttgctct caactattct ttcttaagcc tcaaatccgt
    43441 ttttatctga tacgtctttg aaccaatgac ccgatttttt gattgttctt tttccatctt
    43501 gtgctaagtc tactgaaata aagccatatc gattcttata tgcattggtc cacgaccagt
    43561 tatccataca tgtccacata tgatagccta aacaattggc accttcttga atggcttgat
    43621 ggacatattt cagatgagat tggacaaatt cgatccgata atcatcgtgg atcatgccat
    43681 cttctttgat aaatcgtgct tcgttttcta cacccatacc gttttcagaa ataaagcagt
    43741 cgatatttcc ataatgatct cgtacattga tcaaaatatc atagattcct tgttcataga
    43801 tctcccatcc gcgataagga ttcattttac gaccaggcat ttcataattg tcgaaataat
    43861 cctcaggcat tggaccgttt ttagtatcga ttggtgtttc ttttgctttt acccggcgtg
    43921 gctggtaata attgatacct aaaagatcta ctgtattttc tttgataacc gtcaaatcct
    43981 ctgcatggat cacaggcatg agatcaagtt cttttactat ctcaatcaat tcacttggaa
    44041 actcgccttt tacagctgga tcaaggaatg aacgattgaa gaacgcatct gctatttgcg
    44101 ctgctttaac atcttcaggg tttgattcgt ctcttggata gcttggtgtc agattcaaga
    44161 tgataccaat aacaccacct tgctccattt catggtaaga tttgatggct aacgctgaag
    44221 ccaacgcttc atgatagcca acttggatcg cttttttcat atctgactct tctggataat
    44281 gccatcgata taaataacct gcttcgactg gaacgattgg ttcattatga gtaaaccatt
    44341 tttttacttt cgaaccaaac aattcaaaac aacttttggc atatttttca taggcttcaa
    44401 ccgtttctcg gttcaaccat ccgccttttt cttgcagata catcggcata tcgaaatgat
    44461 acagattcac aaagggttcg ataccgtttt caatcaactc atcaaaataa cgatgataga
    44521 aacgaacggc ttcttcgttg attgttccag attcatcagg tatcaaacgg ctccattgga
    44581 tagatgttcg aaatgaatta tgcccagtct gtttcatcaa ctgtacatcc tcttgatact
    44641 tcgtataaac ttgcgaggtt ttttcaggtc ctacttgatc aaagaacttt tgcggttctt
    44701 ttttgtacca ataatcaaaa atgttctctc cttttccatc gcctgaaatc cttccttctg
    44761 tttgtgggcc acttgctgca gaaccccacc aaaaatttgc tggaaattga tattttttca
    44821 tcaaagcacc tctttttctt ttttagttcg ttttagttga ttccatgtat gatattttcc
    44881 atcacaatca ctttttcatt ttcttcctct tcttttttga ttcgttttaa cagctgttct
    44941 gctgctcgtt ccccgatttt atctgttggt ggcgtgatcg tgattttcgg aaggatactc
    45001 agatcgtttt tatctaaata atcatagtga ctgaacgcaa tatccttccc gattctgaca
    45061 ccttcttgat tgaaacattc gatggcacca atcgacatat tgtaattgct gacaaaaata
    45121 gcagttgccc ctttttctaa taatgctttt gccccgtaat agccgctttt tctagaatag
    45181 tctcctgtaa agacctctaa ttggatcgat ggatcatttt ggaccgcttc tttggctcct
    45241 gctaaacgtt ctcttgctac gtagtctgtt tgagtggcag caataaatcc gattttttta
    45301 tgtccttgtt caccatgatg agcgatcact tttgctacac tttctcgatc attcactaat
    45361 attgtatcaa tacctgctgg tccttctgga caattcaatg cgataactgg aatactcatt
    45421 tttgtgagca tttcaaatcc agaccactct tcttcggata agaagacaat cagcccatcg
    45481 atcacatgct tcatcaggta ttcaattttt tgttcaattt tttctggctg atcggcaaac
    45541 ccgcttaata ataagctata cccattttct tcagctactc tttcgatccc tgagacaact
    45601 tccgacccaa aacggctgga aatcccgttt atcaaaagtc caatagagaa gcttcgatta
    45661 ttcttcaacc ctttagcaat gatgttttct ttatagtcca acgctgaaat cgcttctgct
    45721 atttttttca tgttcttttc cttcagttgc tgaccattca agtaacggga aactgttccc
    45781 actgaaacac cagccatctt agccacatca cggatcgatg ccatacattc tcactcctgc
    45841 cagttacatt tgatttctct ttcttgttgt tcgattatga tgaccgcatt ctttgcatag
    45901 tgttgcttgt catcaatatc catataacta aagtcttcag tagagcaact tcctctcact
    45961 attaattata cttaaacaag tatttgaacc ggttcatttt taatatagca tttttttaaa
    46021 agattgcaac acttttttaa aacgctttct gaaagtggcg gattttttgc ctttttaaaa
    46081 ttttattcta aaatttactc tatttgtggg tgttttgtgc gtttttctct tccatccgtt
    46141 tgaagtttta cattccaatg gtataatttc tttatcctat caaatggggg aagtattttg
    46201 aagaaattaa tcgtactatt tattttgtct gcgggacttt tgacattgac tggctgtgat
    46261 ttgtttggcg gctctgacca atcctcggac gaaacaaccg attcttctgt cttaaaagcg
    46321 gaaaaagcag ataccaccaa cagtgtatat acgctaaaag tacgtaaaga tatggattta
    46381 tccgcgggac agttgacatt tacagataaa aaaatggaat ggacacgcac gtatcagaca
    46441 caaggaactg attcttctga atcatctgaa gaaaaaacag tgctgacaga tatcaagatc
    46501 actaccaaag atgacgaata tattatttca ggaaaagaaa ataaaaaaga aacaacagtc
    46561 atatttacaa aaatcggtaa ttaccgtttg aaggatgagg atggcaacat ctacagtcta
    46621 tagtgccgtc tgttggatct tcttttgttt ctccccttct tttctgctat agtaaagaag
    46681 caagatgatt gtaaaggaga cgaaatggat gatcgatagt cctgattatt gtacatgtaa
    46741 taacaacaaa aatatccata cagaatttaa tgaccaagga agcttgagca tatgcgacaa
    46801 ctgtcacaaa ctagtcaaag attcatttaa aacatttgaa tcttatcctg ttcaaaatta
    46861 tgtaaaaagt agaagaagat aaacaaatcc aagcaaaact cactatggct gatttttatg
    46921 aacttcaaac tcagcttctt cctttgatca agcttcatca gtaaaacaaa cagtggatca
    46981 tttcagttta caaatcaaag agtaaagatt tatttatttc aaatatttga atataaaata
    47041 accaaacgat agaaaggctt attctacatc acgtagtaat ttttctttcc cgtcgtttgg
    47101 ttttttattt ttagcctaat tcttgacctt ttgcatgctg gttatccgta aaatattttc
    47161 cagcaggctt tccttccaat tcagctaatt tttcttggat ctgacggatc accttttttg
    47221 catcttctgg attttctaca aaatcgaatt tatccccatc cacttgtatt ttaggacata
    47281 catcaaaatc ttcataccat tgattatagc gggtgttcaa cgtttcataa taggtatata
    47341 acgtctcatc gctttctagc tgttcatagt cacgcccacg ttttttgata cgttctagca
    47401 tcgtgtccaa agaaactttg atatggacca tcaaatcagg ccgttttttt ggagcaacat
    47461 catctaactc gtttagcatc gtatctaata agctatcgta ctgctgtact tcgatatccg
    47521 ttacccgacc taaatcagcg tttagatgga acaacaggct atcttcatag atggatcggt
    47581 ctagcacatt gtcatcgttc actaaagcat ttttcattgc taaaaatcgc ttgttcaaaa
    47641 agaagatttg caataaaaaa gcgtactgct caggatttga ataaaagagc ggcaatactt
    47701 cgttatcttc gacattttca taaaatggtt tagaatcaat ctcttttgca agcatatccg
    47761 tcaaactgct cttacctgca ccaatcgttc ctgaaatcaa aatcactgac atacaaccct
    47821 tcctcttcta taagtactcg cctacgtaag tttcaacagg caatgtttca aaatcatatt
    47881 cacgtacagc ttggtctgat tttaaatacg tatggagact ttgtgtaatc atcaagttcg
    47941 taggtaccgt ctcttcttgt acaatgatca tcggtttttt caacatagtt gccacaccta
    48001 attcgtaagc cgttcctgga tcgattgttt gatgctcaaa gtcaaggata gcaacaacaa
    48061 attctgcatt tgtcagttct tccatatctt tttcgtatac ttcctgcgcc catccagcgc
    48121 tgaatagttc ataattgctt tcttggtgat gacgtggaga gtagaaactt gtgactgttt
    48181 tgttttcctc caacgctttt tcgatacgac ttactcgatc gatttgctcc tctgaaaaaa
    48241 atggtcctgc taaatatatt tgactcatta ttttgcctcc ttcaataata actgattttt
    48301 aatagatact gctcgtaatt cattgaccag ttccaatgga ttgttggaaa agattacttg
    48361 atccaaccat tttttatcca taaaatcctc ttttgcaaaa gtttctaata attgttgcaa
    48421 gccgctgtaa taaccgccaa tattcaatat tccgattggc ttgtcgggcg tcagaccgat
    48481 tgctatccaa gaaagaagtt gtgagaattc ctccaatgta cctagacctc cagggaaaac
    48541 gaggacagca tctccctttt caaataataa tacctttctt tcatcgatcg tttctgtagg
    48601 aataaatgta gtaacatctt ttccaggcaa ttcagtttca aaaagcccat tcggatagat
    48661 acctgtgact tttccgttat tttccagtac tgttttacta atcactccca taatcccgga
    48721 gtatgaacca ccgtaaacca gttcgatttc ttcttgtgcc atgtattttc ctaacgtttg
    48781 cgcaataaat ttgtaacttt ctttattacc aaaacgggaa ccgcaaaaaa cagtaagttt
    48841 cataaaaaaa cactccctaa ctcaaaagtc tatcttcatt ctacacgtgt ttttttaaaa
    48901 taaaagacta gattttgtag ttttttagtg gtatatagga tagacgccac tatatatag
//
LOCUS       DACDKM010000010        47951 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20834_2016
            SAMN12640370-rid9891663.denovo.010, whole genome shotgun sequence.
ACCESSION   DACDKM010000010 DACDKM010000000
VERSION     DACDKM010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640370
            Sequence Read Archive: SRR10040891
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 47951)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 47951)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742835.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742835.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:04:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,012
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 29 of 75
            Pseudo Genes (incomplete)         :: 33 of 75
            Pseudo Genes (internal stop)      :: 19 of 75
            Pseudo Genes (multiple problems)  :: 5 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..47951
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640370-rid9891663.denovo.010"
                     /isolate="E20834_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(38..1189)
                     /locus_tag="IV334_000584"
     CDS             complement(38..1189)
                     /locus_tag="IV334_000584"
                     /EC_number="1.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HAP8942678.1"
                     /translation="MRAVHFGAGNIGRGFIGEVLAANGFEITFVDVNHTIIDALNERK
                     EYEIELADESKEKITVKNVKGINNQTNPEKVIQEIAEADLVTTAIGPNILPFIAELIA
                     KGIQEREKEGSTTSLDIIACENMIGGSAFLEKEVYKYLPETSFTDKYIGFPNAAVDRI
                     VPLQHHEDPLFVQVEPFKEWVIDDSQRKNKKIQLKGVLYVDDLEPYIERKLFSVNTGH
                     ATVAYTGALLGYQTIDEAMQDALVVAQLKSVLQETGSLLIAKWGFDAEQHHAYIEKII
                     HRFQNKNISDAITRVARTPLRKLGYQERFTRPVRELQEHNLTCPHLTATMGIIFNYYD
                     PEDEQSRQLHEMKIHENLEQLIQEVTGINDPKTIGNIKQNVNRYAKQVA"
     gene            complement(1202..1639)
                     /locus_tag="IV334_000585"
     CDS             complement(1202..1639)
                     /locus_tag="IV334_000585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAP8942679.1"
                     /translation="MQSLPFDMIELNRSFKTKEEAIRYCGQMLVEAGCVDEEYIDAMV
                     DRDKMLSVYMGNFIAIPHGTDEAKKYVKKSGICVVQVPDGVDFGTEEEEKIATVLFGI
                     AGVGDEHLQLVQQIALYCSDMDNVVQLADALSKEEVTENLAIA"
     gene            complement(1654..3420)
                     /locus_tag="IV334_000586"
     CDS             complement(1654..3420)
                     /locus_tag="IV334_000586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IICBA"
                     /protein_id="HAP8942680.1"
                     /translation="MERTIPKNNISLKARVQKLGSTLSSMVMPNIGALIAWGVLTALF
                     IPDGYLPNEALATMVSPMLTYLIPLLIGYTGGKVIAGDRGSVVGAIATMGVIVGTDIP
                     MMLGAMIMGPLGGYAIKKFDQLFQKRIKSGFEMLVNNFSAGLIGFGLALLGFSAIGPV
                     VDALTQAMAKGVEIILSAHLIPLTSIFIEPAKILFLNNAINHGILTPLGTEQVAEAGK
                     SILFLLEANPGPGLGVLLAFTLFGKGAAKSSAPGAMIIHFLGGIHEIYFPYVMMKPLL
                     FLSVIGGGMSGSFIFQLMDAGLRAPASPGSIIAILAMTPFNSYLPVILGVIVAAAVSF
                     GISAVILKADAKDTNEDFEKSVQETKAAKQASKGAQGIPQGATFSGVQKIIFACDAGM
                     GSSAMGASILRKKVQEADLPQTVTNQAISQLTDDSNTLIVTQVELQERAKQKAPSARF
                     VSVENFLNSPRYDEIIQELSSKQKTSVSEIENEPAEEFLSANIKQIKEILLVHDDRSG
                     SATMGMTVLKDILDKNHLTIPLRKVNIQDLKDEPDSLIVAKESLAEKVQMAAPQSIHL
                     SVNSMVNPQKYESIAQYLKQTA"
     gene            3684..4361
                     /locus_tag="IV334_000587"
     CDS             3684..4361
                     /locus_tag="IV334_000587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP8942681.1"
                     /translation="MTLLIKNMNMKRGKKRVIRQLNLLVESGEVAALIAPNGFGKTTL
                     LNGIAQLLPTTYDQLSLHNISPTAYTDYRRSFFFLESTNQLSDYLSAVDYLTLIKYYW
                     QSKVECQTVFQRLKMTEYLTLPIGKMSLGMKQHVLLAAYLISDSSVMLFDEPLNGLDP
                     GSIDILNSLIRQWQKEGKTILLSSHAIANIQSICSKACFLKNGEIVTESSNMEEILAI
                     YRKLYEV"
     gene            4528..5490
                     /locus_tag="IV334_000588"
     CDS             4528..5490
                     /locus_tag="IV334_000588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP8942682.1"
                     /translation="MIETLKEDENEKEFVESLEKSNKLIEAILHAKNTGNVQQMVEAT
                     YHYEKDILDRLISGQRQGIPIIEQQKRVELLRYMKEHQIQRYSIFDLPAHLPLANYYE
                     NIFSGMISSFLILCITALFLSSIISYEKRKQVISLVNLLPDSMVKKHSIRFTIYYGAA
                     MLSLVMPFLIVSILVIIKNGLGDFRYPVGTIIGQEIRILPMYEYLFQSFLFLLLWVLF
                     LSTISFLLSALFEHSLVNLLGTLLCLFLAEYRLFSSIGWIESISHYLPTSYVDFQNVI
                     IGGDIFSPLASEQVTFMNGILTLGIWSIVLLFIGMGTIYIKKSY"
     gene            complement(5690..6130)
                     /locus_tag="IV334_000589"
     CDS             complement(5690..6130)
                     /locus_tag="IV334_000589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAP8942683.1"
                     /translation="MIQLPAEKILLDQSYQNWKEALTEIGQWMTQQGGIKPAYIQSMI
                     ERQEKASVYIGNFVALPHGTDKDEEVVEEGIYLVQVPDGVDFGTKEEPKIATLLFVVA
                     LKKEHQLAVLQDLAFFCSDIDQVMALSDAKTIEEVQMIFEHGHI"
     gene            complement(6141..8174)
                     /locus_tag="IV334_000590"
     CDS             complement(6141..8174)
                     /locus_tag="IV334_000590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HAP8942684.1"
                     /translation="MYLSPREKQLLAEFLSSPSPVSIQKMMNLLKVSKRTIYRELDNL
                     EVSLKSVNASLKKAARGSFVIEASEEVLEQLKENTDGKSNDLSTAKRQHAILAELLLT
                     TEPLSLAHFMESYRISNTTFYADIKQLEESIRQLPLEIIRNQGYEIEGPEKYRRLLTA
                     NVLELEINEYELFHSISFDASLNYFFRFVDPQHLSLARKVIGEELIQKKTNLSDRKLE
                     HLVLMLTLTMERVTKNHLLTNETYNSLANKELLQTAKRLFSRIASETKQLYPVNEIVF
                     FAGLLNDFTNSFDQNFFEETFDTQLAYLVKQLIQEVSEETNVHFYEDETLYKMLLTHL
                     SGVFSRAVLQEETLTNPILEKIMVQYEEVANAIRKAVERIFQEKKLSEEEIAYMVLHF
                     ANSLERSPKESNISIAGFSPSGLASTSMLELRLKRYFPFIQQIHFYRIADLKKIDLQE
                     EYDVVISTSILPGYGGKYILVSPLLLEDEVKQLKEAFSHIEKKKRQKITYNSNNEWMI
                     EDTYEETMHFMDQINQLLGRFFIQELDNPETISETIRSLFPYFPEGLVTDEEVVHRRL
                     LKRYEQAPIGIPHTNMGLFHTSSSLISKPIFCIFNLKHPLTIEGMDRQPVDLSRMLVM
                     LAPSPIQEKDGILLGKISGAIIMNDLNTEIFRSGNKEIVYQLLAKILIEEIKK"
     gene            complement(8176..9879)
                     /locus_tag="IV334_000591"
     CDS             complement(8176..9879)
                     /locus_tag="IV334_000591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP8942685.1"
                     /translation="MEYKWKANNSSRVSMKQLRKAGHHLNEMIMPNLSLFIAWGILSL
                     ATKGVSGDLHTTLEEVCHWMIQLLLPILISYSGGKSVGGQKGAVAGAIASLGLIARSN
                     APQIVGAMIIGPLAGIVYEQFVSRFHVKFKAGYEMLVSNLLVGLTGSVICIIGIVFIE
                     PVLNSVHLLLASVVSWLVTMNLLPLVSLILEPLKVLFFNNAINHGVLTPLGIEFSQTV
                     GYSSLFLMEANPGPGLGILLSILCFAEKQEKVNASGALMIHAIGGIHEIYFPFVLLRP
                     YLFLAVMAGGASGTLIFQWLDTGLAAPVSPGSLLVIFANAPYPQLAGVSIGIAISTFV
                     TFICAAILLKLPNKEKENKTVKERGEILEPSIIHSVIFACDSGMGSSAMGAALLKKKL
                     AAEKIDLSVGYSSIYELNNQVNQLVIVQHELAGLAREKVPQSQIYVLDHFLETDILLS
                     RIKEAIQFPKREEKKEIADITEQKKSPANIVFLYKNNRRGSQTMGMTILSQLAKKAKK
                     QFVISKESIEKIKIQEETIYIAQRELAVTEQLEEKIPRLVLVEHWVATNEYEKLVKGE
                     I"
     gene            complement(9973..10923)
                     /gene="manA"
                     /locus_tag="IV334_000592"
     CDS             complement(9973..10923)
                     /gene="manA"
                     /locus_tag="IV334_000592"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase, class I"
                     /protein_id="HAP8942686.1"
                     /translation="MQEPMFLKPVFQEKIWGGSRLRSVFGFDIPNDKIGEDWAISAHP
                     HGVSVVENGEFKGKRLDELWSEHKELFGHPSEPVFPLLIKILDAEDDLSVQVHPDDAY
                     GMKHEGELGKTECWYIIDAEPGAEIIYGHHAKTREELAEMIKDGRWDDLLKKVPVKKG
                     DFFYVPSGTIHAIGKGIMILETQQSSDTTYRVYDYDRKDAQGQTRELHIQQSIDVTTV
                     PAKTPELQIKEVRKGNSSIVTYLETEFFNVYEWDIKGITSFKKQAPYTLMTVIEGAGD
                     LVVDGKTYPLEKGTSCIIPNGVSEWAVQGELAIIATEPGK"
     gene            complement(10962..11987)
                     /locus_tag="IV334_000593"
     CDS             complement(10962..11987)
                     /locus_tag="IV334_000593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase family protein"
                     /protein_id="HAP8942687.1"
                     /translation="MYPHTKQLILKKMEEGFYPGVAVAFIKENQVETFTAGFAQVVPE
                     KQLMTEDLLFDAASLTKVVATTSLVLRLLEDGKIELDQPLHKYLPEFENQTITLRHLL
                     THTSDIKTWIPNRDKLSQEELIAAYLKLEPGEQLGKEVKYTDAGTILLGLMLEEIYQE
                     TAVEIFQEQVLEPLGMMNSLFLPFPSKKIVPTEQLPSGEVLRGQTHDPKARVLAEHAG
                     NAGLFIDLKDSITFVQMYLNKGKIRNGRFLEEETIDSLLQDQTPKKNGYRSLGWDLKK
                     DVEDGHAILFHTGYTGTFFLIDIKTQEALIFLSNRVHPVDHRAEYLICRDEIVENFLK
                     EKAVLPK"
     gene            complement(12196..13065)
                     /locus_tag="IV334_000594"
     CDS             complement(12196..13065)
                     /locus_tag="IV334_000594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extradiol dioxygenase"
                     /protein_id="HAP8942688.1"
                     /translation="MKDFQLGAATYLKSVAIRVKDRDKMIDFYKNVVGFDLKREENEL
                     AILGTEEAGSEMLLLEESPRANDHMGVIKKLNRFSLIIPSTEEMADILCRIRKMNYPI
                     KEALEDHGRMGILLVDPEENELEIYHEENKNDQREIPETMDQEALLRKSEEKFSKLST
                     GSYFEKIHLNVTNLTKERQFLNHVLGLKVHNEEEGIHVLNEGDFHVGLTEASGGAIDL
                     PTDEVIGLDFLKFMIDTESMEGLIAHLQQLSVDFFLDKKHTVLTIYDAIGIEWWFVSE
                     PKEKNKTDIRCDA"
     gene            complement(13524..15416)
                     /locus_tag="IV334_000595"
     CDS             complement(13524..15416)
                     /locus_tag="IV334_000595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP8942689.1"
                     /translation="MSKTNTRPSHGPAGGPGHGGPGRNLGVKGEKPKNFWGTVRRLFG
                     YMSKRMVAIIAVFVLAIAAVIFQIQTPKVLGKATTEIFKGVMAGAQQMQQGQQVTKFP
                     IDFDKIAQIIATVIVMYLISAVFNFLQQFIMTRVSQRTVYELRHDLEAKMNRVPISYY
                     DTHTNGDIMSRAINDMDNIASTLQQNLTQFVTSAVTLIGVIVMMLSISWQLTLVALLM
                     IPLSLIIVMIVAPKSQVFFADQQKSLGLLNNQVEETYGGHTIVKTFNHTEKDQEVFEK
                     ENQNLYAAGWKAQFISAIIMPLMNFVKNLGYVFVAVLGGVKVANGNMTLGDVQAFLQY
                     TTQFSQPITQLASLMNTIQSTVASAERVFEVLDEEEMSNEKSGLPEEKDTPYKVRFEN
                     VQFGYTPDNLLMTDFNLDVKPGEMVAIVGPTGAGKTTLINLLERFYDVSGGSIRYDGV
                     DTRDLSRDELRAQFSMVLQDTWLFTGSIYDNIKYGNDDATDEQIIEAAKAAHVDDFVR
                     KLPEGYNTVLNEDASNISQGQRQLITIARAFLANPDVLILDEATSSVDTRTEILIQKA
                     MNRLLENRTSFVVAHRLSTIRDADNIIVMNHGSIVETGNHDTLMEKDGFYADLYNSQF
                     SEEEAS"
     gene            complement(15413..17140)
                     /locus_tag="IV334_000596"
     CDS             complement(15413..17140)
                     /locus_tag="IV334_000596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP8942690.1"
                     /translation="MLKIVKRISLTSAIAAAVFMVIQVLADLYLPTLTSNIIDKGVAQ
                     GDVDYIWHTGFVMIGFSLISILAAIANTFFATRESQKLGKQLRTDVYKKSESLTKDAF
                     DKYGTASLITRTTNDVTQIQMVTQMFLRMMINAPITLIGASILAYQKDHQLTKIFLVV
                     IPVMIILIGGIMYFAVPLFKSMQKKTDRLNLVFREGLTGVRVIRAFDKTRFEENRFDL
                     ANKDYTNTAIKVNTIVALMMPMMTLIMSGTNVAITWFGGHYIADMTLEVGNLIAFMTY
                     AMQILISFMMLSAIFIMVPRAQASADRINEVLDEKIGIHDPENPKTVSFAGKNATLAF
                     NHVNYRYHGAEKLALEDIDFQAKSGEIVAIIGGTGSGKTTLVNLIPRLYDIESGSIQI
                     NGTDISDMTQYNLRELMGFVPQKAVLFSGTIRDNMQYGKPDATDEMIWKALEIAQAKD
                     FVSEMEDGLDSHVEQGGGNFSGGQRQRLAIARALVKTADIYVFDDSFSALDFKTDANL
                     RQALKTNMKESITVLVAQRVSTVMDADMILVLDEGKLVGKGTHEELLATNETYQEIVH
                     SQLREEDLA"
     gene            complement(17156..17806)
                     /locus_tag="IV334_000597"
     CDS             complement(17156..17806)
                     /locus_tag="IV334_000597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAP8942691.1"
                     /translation="MPTQTFFHLPKEKQKRLIEAARIEFSRVPLKEASIANIVKLADI
                     PRGSFYQYFEDKEDLYFYYFETMRRDSSKDMLQLMKESDGDLFKGFESYFSKMIYEIL
                     EGENASFYRNLFMNMDYRASRKVAPHTSRQKTPHDPHKEHKQDAKELLELIDRTKLKV
                     KNDHELELLIQLVMNTVFTTIAHSYRLFSTSEDYSVEQAISEFNLKLSWLKNGVYK"
     gene            18010..18306
                     /locus_tag="IV334_000598"
     CDS             18010..18306
                     /locus_tag="IV334_000598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HAP8942692.1"
                     /translation="MAQSISVQDFNKLPLDADSIVLDTRNTLDYTKNHLSHSLSIPAA
                     ILPRQLKNLDPKKLYYILSYTGRRSEALADQLTANGFRAVYIIGGMEALQSSVA"
     gene            18478..19098
                     /locus_tag="IV334_000599"
     CDS             18478..19098
                     /locus_tag="IV334_000599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-dependent NADH-azoreductase"
                     /protein_id="HAP8942693.1"
                     /translation="MASVLVVKGHPLTAEESRTVKALTSFLTSYKENHPDDEVTVLEL
                     YRDDIPEIDEELLSGWEALRAGAEFTSLSESQQQKIARFNELTDQFLAADKIVIANAL
                     WNLNIPTKLKAWFDTVNVAGKTFRYTENGPEGLVTGKKALHIQSNGGVYNGQDFASQY
                     VKGILNFVGIDQVDQLFIEGIDYDPDRADELMQNALDKAAALGKSF"
     gene            complement(19190..20356)
                     /locus_tag="IV334_000600"
     CDS             complement(19190..20356)
                     /locus_tag="IV334_000600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HAP8942694.1"
                     /translation="MEGKNKKNRPVSWFWRWFLNNQVVTALLVVLLILLIVFLFTKVS
                     YLFAPIWQFLAIVGLPIILAGILYYLMNPVVDYFEKRKVPRLYSIIGLFVLVVALIAW
                     GSVVIIPKIQEQTVSFISNFPQYVDTIDNKLTEILRDPLFNQFREQLETAGDKFMNSA
                     GDMIQDISKSTVQSLGSFVGAVATILVALLTMPFILFYLLKDGKQLAPYFVKFLPTRM
                     QKPTLNVLSEMNSQVSSYIRGQLTVAFAVAIMFMIGFAVIGLEYAVTLGIIAGFLNLI
                     PYLGSFLAMIPAIFLAIVAGPFMIIKVLIVFAVEQTIEGRLISPLVLGNQLAIHPVTI
                     LLVLLTSGKLFGIIGVILGIPVYAAAKVVITHVFEWYTTISSLYEENPLKKQKE"
     gene            complement(20368..20721)
                     /locus_tag="IV334_000601"
     CDS             complement(20368..20721)
                     /locus_tag="IV334_000601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HAP8942695.1"
                     /translation="MIQAKITEIGTEAINKEEPMLILFDKTATAALRKYSVIQEVSKD
                     EPFSLKAGGTIAFDDQVYTIDYVGPMANGNLETVAHVSLIFDACPEEGQIANGIYLHP
                     HRLPAIGLGSQIFYN"
     gene            20849..21871
                     /locus_tag="IV334_000602"
     CDS             20849..21871
                     /locus_tag="IV334_000602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactonase family protein"
                     /protein_id="HAP8942696.1"
                     /translation="MIEKIILGTYTRRESKGIYTIDLDTEKEELANLSLAVEENSPTY
                     VAKSKAGNLYTVTSVDGLGGAGAYDKDFHFLNAVTESGAPLCYVAVDEARQLLYGANY
                     HKGEVNVYHILGDGELKAADSIFHQEATGPHKNQDHAHVHYTDLTPDQRLVVCDLGTD
                     RVYTYDVSENGKLNLVTTFTAEPGTGPRHLVFHPNGQFAYLFGELDSTVRVLSYNKET
                     GAFEELQKISTLPEEFDGENGGAAIRISNDGKFLYASNRGHNSIAVFAVFEDGSHIQN
                     KQIISTEGDFPRDFALDPSNNFVVCANQNTDNLTLYRRHPETGLLELLKKDVYVPECV
                     CVYFDR"
     gene            complement(22310..22744)
                     /locus_tag="IV334_000603"
     CDS             complement(22310..22744)
                     /locus_tag="IV334_000603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8942697.1"
                     /translation="MLPNIGYYVQSIDQIVKETEAIGEGMHPKYEVIRNAIDEGKTSE
                     LDKSTLEEIILLFEEGTKKYRAMLKKVSTLRPPAKAMGIHKNFEKAYLSYVAGCEEMI
                     QSIDLESGVDEDLFNTSEAKQDQATDQLTAAIQKMTNLLLKK"
     gene            complement(22854..24713)
                     /locus_tag="IV334_000604"
     CDS             complement(22854..24713)
                     /locus_tag="IV334_000604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HAP8942698.1"
                     /translation="MKRYISRLSTKQKKILKGTAAIISIFFLVVFSNLFLQWCQNDLS
                     VDLALKFAFSWHTEKFFLACLVLLIILIFLIALAGSVPLGSLTYVVAIGVLGFANYMK
                     MSYRQEPIYPDDLKMITEIGLLKDMTGTMLFTVILAAAGTVIGLFCWYMFRSLKKGRR
                     FQLIRLTTLLVAIGLLGYISNFNNPDNLLRKAYNKTALWIPYSQKMNYYNTGFIGGFL
                     YNLKVEPMDEPEGYSKEKIKEITEKYQKLADEKNKTVEEESPNIVFVMSESFSDPSRL
                     NGVEVSGEPLADYYEVADQTYSGNMLSQNYGGGTANIEFEALTGFSMALFNAQLTTPY
                     TMLVPKMDQLPSIVSALNAQSYQTTAIHPYNTSMYKREDVYQTLGFDQFISERTMTYT
                     DTIENNPYISDESAYKEVMTLLKEEKTPQFIHLVTMQTHMPYDGKYDNLDYPAKTSDG
                     SGTLDLENYLQDISYSSTALKQFTEELKNLSRRTLVVFWGDHLPGIYSDTIQAKNDKQ
                     TLHETQFLMFDSKGELEKQTTHDAITSPFYFAANLMEQTNQTTNGFYQLLLSLEQELP
                     AFERELYYQNGQWYKEAQFNRSQQEIYDEYQLIQYDIVAGKQYSLADGFFEHE"
     gene            complement(24831..25991)
                     /locus_tag="IV334_000605"
     CDS             complement(24831..25991)
                     /locus_tag="IV334_000605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-3-phosphoglycerate dehydrogenase"
                     /protein_id="HAP8942699.1"
                     /translation="MYHVHLIDAIPEQEIEKLQGEYFKIIDDLPAEGIVIRSTKVKDE
                     WLTPDLLAISRAGVGVNTINVEKASENGTIVMNTPGVNANAVKELVLCCLLLSSRPII
                     EASRMVQTLTGPNILEQAENKRSAYVGRELQGKTIGLLGLGAIGTKVALSCYSLGMDV
                     LGYSIRDAQLDYVRQADLETVLSTSDYIVVMLPLTEDTKGLIDQANIEKMKKDAVLIN
                     VGRSEIVDKYAVMQALEKQHLAKYLTDFPEEEFLENDRILMLPHLGGSTQEALADSGR
                     LAVEALKDYLLFGTVREAVNYPSARMFFQAPFRFTIFYQKKTNILAEIFSLLNENELA
                     IADISRNHKNEHVYILIDIDSTDFEQLNQVKQQLEKISGVRKVRLLKKPERP"
     gene            complement(26298..27722)
                     /gene="araA"
                     /locus_tag="IV334_000606"
     CDS             complement(26298..27722)
                     /gene="araA"
                     /locus_tag="IV334_000606"
                     /EC_number="5.3.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose isomerase"
                     /protein_id="HAP8942700.1"
                     /translation="MLNIGEKEFWFVVGSQHLYGEEALREVKKHAQEMVDELNENGQL
                     PYPIRLQELAVTADTITKIMKEVNYREEVAGVITWMHTFSPAKMWIRGTKLLQKPLLH
                     LATQYNESIPWKTIDMDFMNLNQSAHGDREYGFINARLNKQNKIVVGYWKRPEIQKEI
                     ADWMDVAVAYNESFGIKVARFGDNMRNVGVTEGDKVEAQIQFGWTVDYFGIGDLVQVI
                     DRVSDEEVDQLFEEYKELYTFDYGDYEEKTWEEHVKVQAQQEIGIRRFLEEGGYNAFT
                     TNFEDLYGMKQLPGLAVQRLMAEGYGFAGEGDWKTAAIDRLLKIMARGKDTGFMEDYT
                     YELASGQEAILESHMMEVDPTLAATKPRIVVSPLSMGDREDPARLVFDGKAGEGVVVS
                     MADFGTHYKLLINEVEAFEPTTEAPNLPVARVLWKTKPNFHEGVHSWIQAGGGHHTVV
                     SLNLTTDQIETWAKLVELETVVIR"
     gene            complement(27738..28436)
                     /locus_tag="IV334_000607"
     CDS             complement(27738..28436)
                     /locus_tag="IV334_000607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005873905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HAP8942701.1"
                     /translation="MLEQLKEEVYQANLDLPKHGLVKYTWGNVSAFDPETRYFVIKPS
                     GVSYEEMTADDMVVVDLDNHIIEGKLNPSSDTPTHAVLYRSFPQIGGIVHTHSTWATI
                     WAQAGLDVPAMGTTHADTFYGSVPCARFLTQQEIDSGYEYETGKVIIETFKERKIDPL
                     AIPGVLLHGHGPFTWGKDAQSAVMNAVVLDEVCKMNLFTRQLNSFSEELPQRILDKHY
                     LRKHGKNAYYGQNK"
     gene            complement(28393..30054)
                     /locus_tag="IV334_000608"
     CDS             complement(28393..30054)
                     /locus_tag="IV334_000608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY-family carbohydrate kinase"
                     /protein_id="HAP8942702.1"
                     /translation="MKEVNAQSMLDVIDGRTSLGIEFGSTRIKAVLIDSSFTPIAAGS
                     YEWENKLENGIWTYSLEDIWKGLRVSYRKMAAEVFEIYGENLTTIGSIGFSAMMHGYL
                     AFDSKEQLLVPFRTWRNTITEEAERILTEKFQYNIPQRWSIAHLYQSILNKEEHVKEI
                     NFLTTLAGYVHWQLTGEKVLGVGDASGMFPIDPSTKTYHQEMKNIFGQLIEKAHVAID
                     LDHILPKVLVAGEEAGKLSESGAYLIDPTGNLRPGIPVCPPEGDAGTGMVATNSVAKR
                     TGNISAGTSAFAMIVLEKELENVHPEIDMVTTPSGELVGMVHTNNCSSDINAWVSIFE
                     EFTNSLGMEISRDKLFTVLFNKALEGDTDCGGLLSYGYFSGENITGVNEGRPLFVRTP
                     GSRFDLANFMRIHLASAFGAMRIGMDILKSENAQIDRLVGHGGIFKTPEVGQRILASA
                     MEAPVTVMDTAGEGGAWGIALLAAYMMDRHGKSLENFLAEDVFGQDQGITITPSAEEI
                     AGYQIFMRRYREGIDIEKTAIHQLQEKGVIENAGTTERRSIPSKS"
     gene            30272..31672
                     /locus_tag="IV334_000609"
     CDS             30272..31672
                     /locus_tag="IV334_000609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar porter family MFS transporter"
                     /protein_id="HAP8942703.1"
                     /translation="MKKEKKVSSSFIYFFGAFGGILFGYDIGVMTGALPFLQHDWGLA
                     GKASLIGWITSSVMLGAILGGSLSGLLSDKLGRRKMILLSALIFMAGSVLSASAPHNG
                     SYFLIAARILLGLAVGAASALVPAYMSEMAPARLRGRLSGINQVMIASGMLLSYVADY
                     LLKVLPETMAWRVMLGLAAVPALILFFGVLALPESPRFLMQSGRLEEAKRVLNYIRTP
                     KEAEQEFEQIQLNVKQEKTTGTSWHTLFLEKYRSLVIAGIGVAVFQQFQGANAIFYYI
                     PLIVEKATGHAASDALMWPIIQGIILVAGSLLFLVIADKFNRRTLLKIGGSVMGLSFI
                     LPAVLGTVLDAHTNSLLILLFLCIYVAFYSCTWAPLTWVIIGEIFPLAVRGRASGLAS
                     SFNWIGSFLVGLLFPVMTASMSQEIVFGIFGIICFLGVLFIQEIVPETRGKSLEEIEQ
                     SASKKTYPKRISIHHS"
     gene            complement(31924..33003)
                     /locus_tag="IV334_000610"
     CDS             complement(31924..33003)
                     /locus_tag="IV334_000610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAP8942704.1"
                     /translation="MAKYQDIAEEIKQKILREEYEVNTTIPSEFKLQEIYDVSRHTIR
                     QAIAVLVNEGYLRKEKGSGTYVDDSYKKEKTPLNRTKTIGVITTYLSDYIFPSIIRGI
                     ENELRESGYSLLLASTNNDHELEKECLKKMIAYGVDGLIVEPTKSNQYNPNLALYVAL
                     REQGIPMVMINAAYEELNASYICLDDVKVGFMAAEYLIKKGHQRLLFITKIDDLQGKY
                     RMKGFISACEQYNVRFLTEDIITFTTETRETIAEKTVQRLKEEQALDGIICYNDQIAS
                     LMLDKLTENGYHIPDDLSIVGTDDSSLSHLGNKKLTTLTHPKEKLGQDAARWIVTTIE
                     KGQAQPNILYEPVLVERETVKENEK"
     gene            complement(33089..34048)
                     /locus_tag="IV334_000611"
     CDS             complement(33089..34048)
                     /locus_tag="IV334_000611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 43 glycosylhydrolase"
                     /protein_id="HAP8942705.1"
                     /translation="MMKTLYNNPIVRQRADPWVYKHTDGYYYFTGSVPGYQVIELRRA
                     KSLNELEHAASLIVWQAHEEGPMSELIWAPEVHYINGKWYIYFAASNHKTIRDESHHH
                     RMFVLENQHIDPMNTDWIEKGQIKTKRETFSLDGTTFSSQGKLYYVWAQQDPEITGNS
                     NLYLSEMDNPWTLKGKQILLSIPEYEWEKRGFSVNEGPAVLTRNGKIFITYSGSATDE
                     NYAMGLLWADEHKNLLLKESWHKLSEPVFVSSEKNKQFGPGHNSFTVSEDGCFDILIY
                     HARPEKNQKGDPLDNPNRHANAQCFTWNEQGFPEFGEPVPYAL"
     gene            complement(34060..35583)
                     /locus_tag="IV334_000612"
     CDS             complement(34060..35583)
                     /locus_tag="IV334_000612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-N-arabinofuranosidase"
                     /protein_id="HAP8942706.1"
                     /translation="MDEEVKVKIREDFTVGEISSRLFGSFVEHMGSVVYTGIYEPGHE
                     TADGDGFRQDVLSYVKELELGVIRYPGGNFTSGYDWVDTVGRKEQRPKKIDLAWRGIE
                     PNSFGLHEFFKWISHVQAKPIFTVNLGTKGIDEAKNLVEYCNFPKGTFWSDLRIKNGQ
                     KKPFGVKLWCLGNELDGPWQVGAKSAYEYGRLANEASKVMKLVDDSIETILVGSSTPR
                     LPSYPEWDRIALEEAYENVDYLALHNYIDKYDEEDLTKPPKNERDDTATYLAKAYRFD
                     RQIEEIVATCDYVKGYLRSEKTMYLAFDEWNVHAQPEKRHRDFEVGSPIDWCYFTMED
                     TLLFGSLGLAILRHADRVKISCQSLLVNTIPLILTDAKGQAWRNPTYYVMQHLSKYAK
                     GQVLKQQIICPVYDCEKIKNVPYVDSVIVDNGNQLTIFLINRTNEQQKITIEHDFNLL
                     DQGEHYTLTSSDLLDKNTREEPEKIKPEINYFSIEKHDELNVGIEKYSWNVIRIKKM"
     gene            complement(35593..36156)
                     /locus_tag="IV334_000613"
     CDS             complement(35593..36156)
                     /locus_tag="IV334_000613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YesL family protein"
                     /protein_id="HAP8942707.1"
                     /translation="MKIVETKFYGQLIVVSDYILLGILWVIVCLPVITIVPATCAVFY
                     VMAQWKGEGSGNIIYFFFQGIKQHFGKNLFLSLLTIIIYFLVNQLLEEQTIVLSISGY
                     VVLLIYSMFLLSWIYVISENKDITFLQIFEQSAWKVFFDFLRNILCCVLLLGFVLLIF
                     LFPPFIFIFAGGVWKLVSIILTIGNER"
     gene            complement(36162..37025)
                     /locus_tag="IV334_000614"
     CDS             complement(36162..37025)
                     /locus_tag="IV334_000614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAP8942708.1"
                     /translation="MAPESSITVTVNSRKAVSGKKIIRTISMLVILLVIAFPFLWLII
                     SSFKHEKDIISFPPRIFADSYTLDNYIKVWTTIPLLDYIKNTVIFAGGTVITSVFFDS
                     LAGYAFARMRFKGKSVLFYFVLLTMMIPFQVFMIPLFIEVNLLGMLDTYAGLIIPRMT
                     TAFGIFMMRSFFITLPDSLEEAARIDGLSEFNIFLKIMLPLSKPTLLSLGIFTLMNSW
                     NDLLYPLILTSSSKMRTLPAGLALFTGQNISFYGPVMAGTVISMLPLLVVYIFAQKYF
                     VQGTAMSGMKE"
     gene            complement(37039..37935)
                     /locus_tag="IV334_000615"
     CDS             complement(37039..37935)
                     /locus_tag="IV334_000615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP8942709.1"
                     /translation="MNGFTKYWNRPDRAAYLFLTPSFLVLLLFTVIPLLGTFAISFTD
                     LNIFFTSRNFVSFENFTRIFSDERAVNSLIHSLYFTVLETPTQIIVGLLAAVALSKNT
                     RFNRFCRSTFYIPVICSLTSIAIIFSMLLDPNVGAIPYLFSQVGLPIPQFFRDPTLAM
                     PTVAIMTVWKNFGVTMTILLTAIQGISPSLYESAQMDGATNRQQFFNITLPQIVPSLG
                     FCILTNLIGSMQVFDQVYVATAGGPQFKTETAVQYIYSRGFTAPYELGYASALSSVLF
                     IIVATIAISTNVYMSRKEKQMK"
     gene            complement(38110..39381)
                     /locus_tag="IV334_000616"
     CDS             complement(38110..39381)
                     /locus_tag="IV334_000616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAP8942710.1"
                     /translation="MKGKKWLIGSLLVAATGILGACGGGSSAESDSDKEVTLTFWNGF
                     TASDGEILQEIVNEFNKTNDKNIKIEMDIMTWANLNEKLPTAISSKKAPDFIALNYPD
                     FAQYVENGAVQPLDDFWEYDGVDKSDFKETAIELGEVEGKQYFVPMQVQGMYMYWNND
                     LFKQAGLDTEQPPTTWEELTEMAPKLTDSSKNVSGFVFNKDGTAPLYNWMLANGGKLV
                     NDDYTKSEFASPENLKTLKAIQKMIHVDKTGPESISGAEMDNLMNAGQLAIEINGPWL
                     NNGLKANEIDYNVTTLPQGSDSDKKTAILDGVGYAIPASTDNNKKEAVYEFLKYWNTT
                     EIGKKWSIENGFPAYLDSVAKDEEVKNDPIVSELSKQMDYAEPYLPGFTQISAINNDI
                     INPLIEKLLAGEDPETLMNDADDAINQLLQK"
     gene            complement(39371..40888)
                     /locus_tag="IV334_000617"
     CDS             complement(39371..40888)
                     /locus_tag="IV334_000617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-N-arabinofuranosidase"
                     /protein_id="HAP8942711.1"
                     /translation="MEATMLISSVKRVGKVDPRLYSSFIEHMGRAVYEGIYQPDHPSA
                     NKSGFRQDVIHLVKELNIPMIRYPGGNFVSGFRWEDSIGPIANRPRRLDLAWRTIETN
                     EVGIHEFYNWCQHVGAEINMAVNLGTRGVDAARNLVEYCNFPKGTYWSDLRRSNGQES
                     PFGIKTWCLGNEMDGPWQIGHKTAEEYGRLAEETAKAMKLVDDSIEVVLCGSSNRQMP
                     TFGDWELTVLDQAYDQIDYLSLHQYYGNQKNDLSHYLARSLDMDRFISEVTAMCDTVK
                     AKKHSKKQINLSFDEWNIWYHSAEQDEQAAPWQIAPPLLEDNYNFEDALLLGCLLITL
                     LKHADRVKIACLAQLVNVIAPIRTETNGEAWRQTIYYPFMQAAVYGQGEVLSPQIRSA
                     NYATEDFEEVPYLESIAVINDKELVIFAVNRGEEEMNLTVHLSELSLEGVIDFSEMSG
                     FDTKATNLPKSEQVKPSHSTNVKAEDGKINCLLKPLSWNVVRCRLSDEGGTKNER"
     gene            41086..41976
                     /locus_tag="IV334_000618"
     CDS             41086..41976
                     /locus_tag="IV334_000618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsR family transcriptional regulator"
                     /protein_id="HAP8942712.1"
                     /translation="MQLQINEESLPVYEALASKTRIKIIQLLSKKKMNVKDLAKELGV
                     SSAITTMHVKKLEEANIIKTEKVGQQKISSLRVDKIDISFPEKIFNAFDTKETSIPIG
                     HYTNYAIEPTCGLATIHDFIGKVDEPRYFMDPRRMDARILWFTSGFVEYQAPNFLNTE
                     DTLEMLEVSVEISSEFPFSNDNWPSDITFSLNGVELGTWTSPGDFADIRGKYTPDWYP
                     DNLNQYGLLKTIRITKHLTNMNGEPLSNITINDIPKEQDTWHLRIEVKDDAKHVGGCT
                     LFGKGFGNYDQDIKLKVYYS"
     gene            complement(42049..42873)
                     /gene="nadE"
                     /locus_tag="IV334_000619"
     CDS             complement(42049..42873)
                     /gene="nadE"
                     /locus_tag="IV334_000619"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-dependent NAD(+) synthetase"
                     /protein_id="HAP8942713.1"
                     /translation="MSLQTEIINELGVKPTIDPKEEIRKSIDFMKAYLKKYPFLKTFV
                     LGISGGQDSTLAGRLAQLTMEEMREETKDENYQFIAVRLPYGEQADEEDAKAALDFIQ
                     PDVSLRVNIKPAVDAQVQVLSEAGVGISDFNKGNIKARQRMITQYAVAGERAGAVLGT
                     DHAAENITGFFTKFGDGGADILPLFRLDKRQGKQLLQALNAPEKLYTKIPTADLEDGK
                     PMIADEVALGVTYNEIDDYLEGKEVPAQAQEKIEAWWNKTQHKRHLPISVLDDFWK"
     gene            complement(42885..44357)
                     /locus_tag="IV334_000620"
     CDS             complement(42885..44357)
                     /locus_tag="IV334_000620"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HAP8942714.1"
                     /translation="MNNTYPDDSLTLHTDLYQINMMQTYWELGRADLHAVFECYFREM
                     PFNHGYAVFAGLERLVNYLENLTFNDSDIAYLRGLEVYPEGFLEYLQNFEFKATVRSA
                     REGELVFANEPLIQVEGPLAHCQLVETALLNMVNFQTLIATKAARIKSVIGEDPLLEF
                     GTRRAQELDAAVWGTRAAYIGGADATSNVRAGKIFGIPASGTHAHSLVQSYGNDYEAF
                     MAYAKTHKDCVFLVDTYDTLKSGVPSAIRVAKELGDKINFQGVRIDSGDMAYISKRVR
                     EQLDAAGFTEAKIYASNDLDEATILNLKMQKAKIDVWGVGTKLITAYDQPALGAVFKL
                     VSIEDDEGKMMDTIKLSSNAEKVTTPGKKQVWRITRNFDGKSEGDYVTLWDEDPREEE
                     AIFMFHPVHTFINKTVRDFTARPVLQDIFIEGKRVYELPALNEIKEYTKENLDSLWEE
                     YKRDLNPQKYPVDLSTECWNHKMAIMERMKKSVAELHTEA"
     gene            complement(44994..45356)
                     /locus_tag="IV334_000621"
     CDS             complement(44994..45356)
                     /locus_tag="IV334_000621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1827 family protein"
                     /protein_id="HAP8942715.1"
                     /translation="MKLIETPVNSNLNIKTFYPKVVEFFFGNTAINYYKLFSLDRTQL
                     LLVDTYDKKQVVMINTKKKITRQEIDYAIHHVLKMTREDVKVHVGVKQELERAGIQFK
                     RPNKDIVVIEQKNTKDSI"
     gene            45579..46331
                     /locus_tag="IV334_000622"
     CDS             45579..46331
                     /locus_tag="IV334_000622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HAP8942716.1"
                     /translation="MEYTIKKMSEISGVSPRTLRFYDEIGLLKPARINSSGYRIYGKK
                     EVDRLQHILFYRTMAFKLDDIQEVLDNPSFDHQKALIKHQQMLLEKRAQIDTLLTTVQ
                     QTLDMYEGGRKMSDKEKFEGFKQQKLKENEESYGQEIRQKYGKKKVTASNEKWMNMDK
                     ETYEEMQTVEKQMLFDLTLYLKEPEDESLADRIFQAHKKWLSYSWPEYQPKAHKGLGM
                     MYEADERFTAYYDERSGHGAAKALNEIIQKKA"
     gene            complement(46471..47394)
                     /locus_tag="IV334_000623"
     CDS             complement(46471..47394)
                     /locus_tag="IV334_000623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HAP8942717.1"
                     /translation="MANIRDIAKLTGYSVSTISRVINGHPYVDEEKRKEILAVMKEVD
                     YIPNANARQLSYGKTKSIGVILPYTNHPYFDQLISGIIEAAFDYDYKVTLLPTGYQKE
                     KERRYLEEFAAKAFDGLIITSRANTLDDLLAYQKYGPLVFCEEIKGKQASCVFIDREQ
                     SITEGLTYLKQQGIKQLGITLGRSGRLSYNSKITLQLCHQLFPSFDESLVFWDCIDAE
                     KGKQAGFFFKELGVDGVFTNSDMVAAGILQSYLQDKTPVVIGRDNLLISELLGFPTID
                     HHLEACGEMAFQLFLTEKKDKVKIPYTFIQR"
     gene            complement(47409..47681)
                     /locus_tag="IV334_000624"
     CDS             complement(47409..47681)
                     /locus_tag="IV334_000624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8942718.1"
                     /translation="MNTYVEITDGTTNNYFYAFVEFKGSVLTLYSFQGLNRNIVKEIP
                     MNEIENLTKDIYWGGQRISFSHEGKMYQFFECGPAVVDYLQENLFV"
BASE COUNT        13978 a         9954 c         8269 g        15750 t
ORIGIN      
        1 aaaaatttcg tcacattccc ctttttcgta tcctatttta agcgacttgc ttggcataac
       61 ggttcacgtt ttgcttgata ttcccgattg ttttcggatc attgattcct gtgacttctt
      121 gaatcaattg ttccaagttt tcatggatct tcatttcatg caattgacgg ctttgttcat
      181 cttcaggatc atagtaatta aaaataatcc ccattgttgc cgtcaaatgt gggcaagtca
      241 agttatgttc ttgtagttct ctcactggac gtgtaaatct ttcttgataa cctaacttac
      301 gtaatggtgt ccgagcgaca cgtgtgatcg catcagagat atttttgttt tggaagcgat
      361 gaatgatttt ttcaatatac gcatgatgct gctctgcatc aaacccccat ttggcaatca
      421 gcaaacttcc tgtttcctgc aaaactgatt ttaattgtgc cacaactaac gcatcttgca
      481 ttgcttcatc aatggtctgg taacctaaca aggcacctgt ataagcgaca gtcgcatgac
      541 cagtattgac actaaatagt ttgcgttcga tatatggttc tagatcatct acatataaaa
      601 ccccttttaa ttggattttt ttattttttc tttggctatc gtcaatgacc cattctttga
      661 aaggttccac ttggacgaat aaaggatctt catggtgttg taatgggacg attcgatcga
      721 ctgctgcatt tggaaagccg atatatttgt cagtaaatga tgtttccgga agatatttat
      781 acacttcttt ttctagaaat gctgaaccgc caatcatatt ttcacaggca atgatatcta
      841 aagatgttgt gcttccttcc ttttctcttt cttggatacc ttttgcaatc aactcagcaa
      901 taaaaggtaa gatattcggg ccaatggcgg tagtaactaa atctgcttca gcgatttctt
      961 ggattacttt ttctggattt gtttgattgt tgattccttt gacatttttt acagtgattt
     1021 tttcttttga ttcatctgct aattcgattt cgtattcttt tctttcattt aaagcatcaa
     1081 tgatcgtgtg attcacatct acgaaagtaa tctcaaatcc attagctgcc aaaacctctc
     1141 caataaatcc tcgaccgata tttcctgcac caaaatgtac tgctctcatt tttctctctc
     1201 cttaagcaat tgctagattt tccgttactt cttctttgct gagcgcgtct gctagctgga
     1261 caacattgtc catatcactg caataaagtg cgatttgctg tacaagttgc aaatgctcat
     1321 ctccgactcc tgcaataccg aacaaaacag tcgcaatctt ttcttcttct tcggtaccaa
     1381 aatctacacc atctggtact tgaacgacac agatgccgga ttttttcaca tatttttttg
     1441 cttcatctgt tccatgagga atcgcgataa aattccccat ataaacagat aacattttgt
     1501 ctctatctac catggcatcg atatattcct catcaacgca ccctgcttct acaagcatct
     1561 gaccgcaata gcggatcgct tcttctttgg ttttaaaaga tcgattgagc tcgatcatgt
     1621 cgaatggcaa actttgcatg ctgttttcct cccttaagct gtctgtttca aatattgtgc
     1681 aatcgattcg tatttttgcg gattgaccat gctgttgact gataaatgaa tggattgagg
     1741 tgcagccatc tgtacttttt ccgctaaact ttctttggct acgatcagtg aatcaggttc
     1801 atcttttaaa tcttggatat tgacttttct taacgggata gttaaatggt tcttgtctaa
     1861 tatatctttg agcacggtca tccccattgt tgctgatccg gaacgatcat cgtggacaag
     1921 tagaatctct ttgatttgtt tgatgtttgc tgaaagaaac tcttctgctg gctcattctc
     1981 aatttcagaa actgatgttt tctgcttgct gcttaattct tgaatgattt catcatatcg
     2041 aggtgaattc aaaaagtttt ctactgagac aaaacgcgcg ctcggtgctt tttgttttgc
     2101 tctttcttgt agttcgacct gagtaacgat caatgtattc gagtcatctg tcagttggct
     2161 gattgcttga ttggtcaccg tttgaggcaa gtctgcttcc tgtacttttt ttctcaagat
     2221 cgaagcgccc atcgcacttg atcccattcc tgcatcacaa gcaaaaatga ttttttgtac
     2281 gccagagaat gtagctcctt gaggaatacc ttgggcacct ttggaagctt gcttagctgc
     2341 ctttgtctct tgaacagatt tttcgaagtc ttcatttgta tctttcgcat ccgctttcaa
     2401 aattactgca gaaataccaa acgaaacagc tgcggcaaca atcacgccca aaataacagg
     2461 aaggtaagaa ttgaacggtg tcatcgctag aatcgcaatg atcgaccctg gagaagctgg
     2521 cgctcttagt ccagcatcca tcaattggaa aatgaaactt cctgacattc ctccgcctat
     2581 aactgataag aataataacg gtttcatcat aacgtaagga aaatagattt catggatccc
     2641 gcctaaaaag tgaatgatca tggctcctgg tgctgaagat tttgctgctc ctttgccaaa
     2701 caaagtaaaa gcaagtaata cgccaagtcc tggaccagga tttgcttcaa gcaagaatag
     2761 aatagatttt ccagcctctg ctacttgttc agttcccaat ggtgtcaaaa tcccatgatt
     2821 gattgcatta ttcaaaaata agatttttgc tggttcaata aaaatacttg tcaatggaat
     2881 caaatgggca gataaaatga tctctacacc ttttgccatt gcttgagtca atgcatcgac
     2941 aactggacca atcgcagaaa aacctaataa cgctaatcca aaaccgataa gacctgctga
     3001 aaaattattg actagcattt caaaaccgga tttgatccgt ttttggaata gctgatcaaa
     3061 tttcttgatg gcatatccac caagaggtcc catgatcatc gcgcctagca tcattggaat
     3121 atctgttccg acaatcacac ccatcgttgc aattgcccca acaactgatc cgcggtcacc
     3181 agcaattact tttccgccag tataaccgat caataaagga atcagataag ttagcattgg
     3241 gcttaccatg gtagccaaag cctcatttgg taaataacca tctgggataa atagagccgt
     3301 taatacaccc caggcaatca gtgctccaat atttggcatg accatgcttg ataaagtact
     3361 gcctaatttt tgtaccctcg cttttagcga aatgttgttt ttgggaatcg tccgttccat
     3421 tttgtctctc cctctctcct tttgatactc taattgtatc cgcttactaa aaagagaaat
     3481 agtcgtactt tggcacgaaa ctttgtgcca aaggtttgag tatgtcagca aaataaaaag
     3541 atttcggtat cagaaaactt caattttaaa taaaactgtt attttaattt ttttataata
     3601 tgcaaacaat aacttttgtt agcatttaat ttagatgttt tccattctat tatgttatta
     3661 aattttatat tggaggccta tctatgacat tactgatcaa aaacatgaac atgaaacgtg
     3721 gtaaaaaacg tgtgattcga caattgaatc ttttagtaga atcaggagaa gtagctgctt
     3781 tgattgcccc gaacggattt ggcaaaacaa ctttgctgaa tggcattgcc caactgctgc
     3841 caacaactta tgatcaattg tcccttcaca acatctctcc tactgcttat acagattatc
     3901 gccggtcttt cttttttcta gaatcaacga atcagttatc cgactatcta tcggctgttg
     3961 attatttgac tttgatcaaa tactattggc agagcaaagt agaatgccaa actgttttcc
     4021 aacgattgaa gatgactgag taccttactt tgccgattgg aaaaatgtct ctaggaatga
     4081 aacaacatgt cttgcttgct gcttacttga tcagtgattc atctgtcatg ttattcgatg
     4141 aaccgttgaa cggattagac ccaggaagta ttgatatctt gaatagcctc atcagacaat
     4201 ggcagaaaga ggggaagacg attcttcttt cttcccacgc tatcgcgaat atccaatcga
     4261 tttgttctaa agcatgtttt ctaaaaaatg gagaaatcgt cacagaatcc tcaaatatgg
     4321 aagagatttt agctatttat cgtaaattat atgaggtgta actatgtctt tacttacatt
     4381 tgaacgcaat ctcttactaa aaaacaaagt aaacttttta tttcctctat tgttggtcgt
     4441 gctctttgca ttcccacttt ttttgatcat aaacttgctt atacagaatt cgatgaactg
     4501 aatcataact atgaagagat gcaacgtttg attgaaacgt tgaaagaaga tgagaatgag
     4561 aaagaatttg tggaaagttt agagaaaagc aacaaactga tcgaagcaat cttacatgca
     4621 aaaaacacag ggaatgtcca gcaaatggtc gaagcaactt atcattatga aaaagatata
     4681 ctggatcgac tgatttctgg tcaaagacaa ggaattccaa tcattgaaca acaaaaacgt
     4741 gtcgagttgc tccgttatat gaaagaacat cagatccaaa gatattctat ttttgacttg
     4801 ccagcgcatc tccctttagc aaattactac gaaaatattt tttcaggaat gatttctagt
     4861 tttttgattt tatgtataac cgccttgttt ctttcttcca tcatctctta tgaaaaaaga
     4921 aagcaggtaa tctctctagt caatctatta ccggatagta tggtaaagaa acatagcatc
     4981 cgttttacca tttattatgg tgcagctatg ctaagtctcg tgatgccatt tttgatcgtc
     5041 tctatactag taataatcaa aaatggtttg ggagacttcc gttatcctgt aggaacgatc
     5101 attggtcagg agatacgaat ccttccgatg tatgagtatc ttttccagag ctttctgttc
     5161 ctgttgttgt gggtgctctt tttaagtaca atcagttttc ttctatctgc tttatttgaa
     5221 cattctctag tcaatctttt aggtacactt ctttgtcttt tcttagcaga gtatcgcttg
     5281 ttttcttcga ttggatggat agaatcaatc agtcattact tgccaacttc ttatgttgat
     5341 tttcaaaatg tgatcatagg aggagatatt ttctcgccac tagcttcaga acaggttact
     5401 ttcatgaatg gtatcttgac acttggtata tggagtatcg ttttattatt cataggtatg
     5461 ggcactatct atataaaaaa gagctattaa aacacaaaaa taataggggc ggtgacaaaa
     5521 accttgtcac cgtcccctat ttcaaataaa cggttttcta gaataaatag ttttcaaaag
     5581 cttgcatccg cagtaataag aacctcccaa caaaaatagc tcctcttatt tttatcttgg
     5641 ttgaagttat tacttgatgc aaggcagctt tttttactgt gaacattggt tatatatgtc
     5701 catgttcaaa aatcatttgg acttcttcaa tggttttcgc atcactgagc gccattactt
     5761 ggtcgatgtc tgaacagaaa aaggctaaat cttggagcac cgctaactga tgttcttttt
     5821 ttaaagccac tacaaataag agtgtcgcga tttttggttc ctcttttgtt ccaaagtcca
     5881 caccatcagg cacttggact aaatagattc cttcttctac tacttcttca tctttgtcag
     5941 tcccatgtgg caaggctacg aaattcccga tatagacact cgctttttct tgtctttcga
     6001 tcattgattg gatatatgcc ggtttgattc ccccttgttg ggtcatccac tggccgatct
     6061 cggtcaacgc ttctttccaa ttttgatagg attgatcgag caatattttt tctgctggta
     6121 gctgtatcat cttttcacct tcatttcttt atttcttcaa tcaggatttt cgctaacaac
     6181 tgataaacga tttctttatt tcctgagcgg aagatctcgg tgttcaagtc attcatgatg
     6241 attgctccac tgatctttcc taaaagaatc ccgtcttttt cttggattgg cgaaggcgcc
     6301 agcataacta acatacggga caaatcgact ggctgtctat ccatcccttc aatggtcaac
     6361 ggatgtttca aattgaaaat acaaaagatc ggtttagaaa tcaatgagct ggatgtatgg
     6421 aataacccca tatttgtatg aggaataccg ataggtgctt gttcatatcg cttaagcagc
     6481 cggcgatgaa ccacctcttc atccgttact agtccttcag gaaaataagg aaataagctg
     6541 cgaatcgttt cggaaatcgt ctcaggatta tccagttctt ggataaaaaa acgaccgagt
     6601 aattgattga tttgatccat gaaatgcatt gtttcctcat aagtatcttc tatcatccac
     6661 tcgttattgc tgttatacgt aattttttgc ctttttttct tctcaatatg cgaaaaagct
     6721 tcttttagct gcttgacttc atcttctagc agtagcgggg agactaaaat atattttcca
     6781 ccgtatcctg gtaaaataga tgtagaaata acaacatcat attcttcttg taaatcgatt
     6841 ttttttaaat cagcaatccg atagaaatga atttgttgga tgaagggaaa ataccttttc
     6901 aatcgtagtt ccaacatgct agtagaagct aatccacttg gcgaaaatcc tgcaatactg
     6961 atattgcttt ctttcggact tcgttccaat gaattcgcaa aatgaaggac catataagcg
     7021 atctcttctt cggataattt tttttcttga aaaatccgct ctactgcttt acggattgca
     7081 ttcgcaactt cctcgtattg aaccatgatt ttttctaaaa tcggattggt cagtgtctct
     7141 tcttgtaaaa cggctcttga aaacacacca gataaatgag tcaacagcat tttgtacaaa
     7201 gtttcatctt cataaaaatg gacattcgtc tcttcactta cttcttgaat cagttgtttt
     7261 actaaataag caagctgtgt atcgaacgtc tcttcaaaga aattctgatc aaatgagttc
     7321 gtgaagtcat ttaataatcc tgcaaaaaag acgatctcat ttactggata aagttgtttt
     7381 gtctcagaag cgatccgtga aaacaatcgt ttagctgtct gtagtaattc tttgtttgcc
     7441 agactattat atgtctcatt tgtcagtaaa tgatttttag tcacacgctc catcgtcaat
     7501 gtcagcatca aaaccaaatg ttcaagcttt ctatcactta gattggtctt tttttggatc
     7561 aattcttccc cgattacttt gcgtgcaaga gatagatgtt gcggatcaac gaatcgaaaa
     7621 aaataattca gtgatgcatc gaaagaaata ctatgaaaaa gttcatattc attgatttct
     7681 aattctaata cattcgctgt cagaagccgg cggtattttt ccggtccttc gatctcgtat
     7741 ccctggttcc gaatgatttc caaaggcagt tggcgaatac tttcttccaa ctgtttgata
     7801 tctgcataaa aagtggtatt actgatgcga tatgattcca tgaaatgagc aagactcaac
     7861 ggctctgtcg tcagtaacaa ctcggctaaa atcgcgtgtt gtcgtttagc agtggataag
     7921 tcattacttt tcccatctgt attttctttt aattgctcta atacttcttc tgatgcctca
     7981 atgacaaatg agcctctagc tgcttttttc aaagaagcat ttaccgattt caatgagact
     8041 tcaagattat ctaattcgcg gtaaatagtc cgtttactga ccttcaataa attcatcatt
     8101 ttttggatag atacaggtga cgggctactg agaaattctg ctaataattg tttttctctg
     8161 ggcgataaat acatcttata tttctccctt cacgagtttt tcgtattcat tcgtagctac
     8221 ccaatgttca actagaacta gccgagggat cttttcttct agctgttcag ttacagctag
     8281 ttctctttgt gcgatataaa tcgtttcttc ttggattttg attttttcta ttgattcttt
     8341 tgagataaca aactgttttt tcgctttttt tgctaattga cttaaaatcg tcatgcccat
     8401 agtttgtgag ccgcgtctgt tgtttttata caaaaataca atattagcag gcgacttttt
     8461 ttgttctgtg atatctgcta tctctttttt ttcttcccgc tttggaaatt ggatcgcttc
     8521 ttttattcgt gacagcaaga tatctgtttc caaaaagtga tccagtacat agatttgact
     8581 ttgaggtact ttttctcttg caagacccgc tagttcatgt tgcacgataa ccagctgatt
     8641 tacttggtta ttcaattcat agatagagct gtatccgaca gaaagatcga ttttttctgc
     8701 tgctaatttc tttttcagca gggcggctcc catagcactt gatcccatac ctgagtcgca
     8761 agcaaaaatg acggaatgaa tgatagatgg ttccaagatt tctccccttt ctttgaccgt
     8821 tttgttctct ttttctttat ttggtaattt caatagtatc gctgcacaaa tgaaggtcac
     8881 aaatgtactg attgctatcc ctatacttac cccagctagt tgtggataag gcgcattggc
     8941 aaaaatcacg agtagtgacc caggcgatac aggtgcagct aatcccgtat ctaaccattg
     9001 aaatatcaac gtgccgcttg ccccaccagc cattacagct aaaaatagat acggtcgcag
     9061 caagacaaat ggaaaataga tttcgtgaat cccgccaatc gcatggatca tcaatgctcc
     9121 actcgcattt actttttcct gtttttctgc aaaacaaagg atagaaagta agatccctaa
     9181 accaggtcca ggatttgcct ccatcaaaaa taaggaagag tacccgactg tttgtgagaa
     9241 ttcgattcct aaaggtgtca atacaccatg attgattgca ttattgaaaa atagtacttt
     9301 tagaggttct aagatcagac tgacaagtgg taacagattc attgtgacta gccaagacac
     9361 aacggaggcg agcaacaagt gaacactatt taaaactggt tcaataaaaa cgattccaat
     9421 tatacaaata acgctaccag tcagtcctac tagtaggttg ctgacaagca tttcatatcc
     9481 agccttgaat ttcacgtgga atctagaaac aaattgttca tatacgatgc cggcaagcgg
     9541 cccgataatc atcgctccta cgatttgtgg agcattggaa cgagcgatca accctaaaga
     9601 agcaattgca ccggcgactg cccctttttg tccccctacc gatttcccgc ctgaataact
     9661 gatcaagatg ggtaaaagca gctggatcat ccaatggcat acctcttcta atgtggtgtg
     9721 caaatcgcca gacacacctt ttgttgctaa tgacaagatt ccccaagcga taaacagact
     9781 tagatttggc atgatcattt catttaagtg atgtccagct tttctcagtt gtttcataga
     9841 cacgcgactg ctgttgtttg ctttccattt gtattccatc tttttcacca tacttctatt
     9901 tattgatgga cggattttat gtagaaaaag cagcaaacga aaaaccgtct gctgctttca
     9961 gctatctggg aactatttgc ctggttcagt cgcaatgatt gctagttcac cttggaccgc
    10021 ccactcagaa acgccatttg gaataataca gcttgttcct ttttctaatg gatatgtttt
    10081 tccgtcaacg acaaggtctc ctgctccttc gattaccgtc atcaatgtat aaggggcttg
    10141 ttttttgaaa gatgtgattc ctttaatatc ccactcatat acattgaaaa actcagtttc
    10201 taaataagtg acgatcgatg aattcccttt tcggacttct ttgatttgta attctggtgt
    10261 tttcgcaggg acagttgtta catcgatcga ttgttggata tgcagctctc ttgtttgtcc
    10321 ttgcgcatct tttctgtcgt agtcatacac gcggtaagtc gtgtcagaac tttgctgtgt
    10381 ttctaaaatc atgattcctt taccaatagc atggatcgtt ccgcttggta cataaaagaa
    10441 atcacctttt ttaactggta ctttcttcaa aaggtcatcc cagcgcccat ctttgatcat
    10501 ctcagctagt tcttctcttg tctttgcatg atgaccatag atgatttctg caccaggttc
    10561 agcatcgatg atataccagc attcagtttt tcctaattca ccttcatgtt tcatcccata
    10621 ggcatcatct ggatgtactt gtacagatag atcatcttct gcgtctaaga tcttgatcaa
    10681 taatggaaac actggttctg aaggatggcc aaacaattcc ttgtgttctg accatagttc
    10741 atctaatctt ttccctttga attcgccatt ttctactaca ctgacaccat gaggatgcgc
    10801 actgatcgcc caatcttctc cgattttatc gttagggata tcaaaaccaa agactgaacg
    10861 taaacgactt cctccccaaa ttttttcttg aaaaactggt tttaaaaaca taggttcctg
    10921 cataattcaa actcctctca tttttcatag ctattatagc attatttcgg taataccgct
    10981 ttctctttca aaaagttttc aacaatttca tctcgacata tgagatactc cgcacgatga
    11041 tcgacaggat gtacgcgatt tgacaaaaat atcaacgctt cttgtgtttt tatatcaatc
    11101 aagaaaaacg tacctgtata accagtatga aaaagaatcg catgaccgtc ttctacatcc
    11161 ttttttaagt cccatcctaa agaacgatag ccattttttt tcggtgtttg atcttgcagt
    11221 aaagaatcaa tcgtctcttc ttctaaaaaa cgaccattac ggatttttcc cttgttcaaa
    11281 tacatttgga caaaagtaat gctgtctttc aaatcaataa agagacccgc gttacctgca
    11341 tgttcagcca aaactcttgc tttcggatca tgagtttgtc cacgcaaaac ctcacccgaa
    11401 ggcagttgtt ctgtgggaac gatttttttg gaaggaaaag gaagaaataa gctgttcatc
    11461 attcctaatg gttccaatac ttgttcttga aagatttcca ctgcagtttc ttggtaaatt
    11521 tcctctagca tcaacccaag aagaatcgta ccggcatctg tatattttac ttcttttcct
    11581 agttgctcac ctggttctag cttcaaataa gcggcgatca attcttcctg agatagctta
    11641 tctctgtttg gtatccatgt tttgatatca gaagtatgtg tcagtaaatg acgcaaagtg
    11701 attgtctgat tttcaaattc aggaagatat ttatgaagcg gttgatcgag ttctatcttt
    11761 ccatcttcta atagacgtaa aactaatgaa gtggttgcaa cgactttcgt cagtgaggct
    11821 gcatcaaaca acagatcttc tgtcatcagc tgcttttcag gaacgacttg ggcaaagccg
    11881 gctgtaaatg tttctacttg attttctttt atgaaggcga ctgctacacc tggataaaat
    11941 ccttcttcca tctttttcaa aataagctgt ttcgtgtgtg gatacatctt tttctcctgc
    12001 ttttctatca gattcttaca gtccaagctt aaagcttaat ctatgaggaa tcaacctttt
    12061 ttacacaaaa aaactgcgcg aaaaaccgcg cagtttcccc cggtatacta ttttctcttt
    12121 ctatctctag aataagcgat agtatgaccg ttcataaaat gacgagacat gcgacaatcg
    12181 ttaagaaaga aaaagttaag cgtcgcatcg aatgtcagtt ttattctttt cttttggctc
    12241 actgacgaac caccattcga ttcctatagc atcatagatt gtgagtactg tgtgcttttt
    12301 gtctaagaag aaatcaactg atagttgctg caagtgagca atcagtcctt ccatgctttc
    12361 agtatcgatc atgaatttca gaaaatcaag tccaatcact tcatctgtag gcaaatcaat
    12421 tgcgcctccg ctagcttctg tcaaaccaac atggaaatct ccttcattca atacatggat
    12481 gccctcttct tcattatgta ccttcaaacc aagtacatga ttaagaaatt ggcgttcttt
    12541 tgttaagtta gtaacattta aatggatttt ttcaaaataa gaacctgttg atagtttact
    12601 gaatttttct tcagattttc ttaataatgc ttcttgatcc attgtttctg gaatttctct
    12661 ttggtcgttc ttattctctt catggtaaat ttccaattca ttctcttctg ggtctacgag
    12721 caaaatcccc atacgtccat gatcttctaa agcttctttg attggataat tcattttacg
    12781 gatacgacaa aggatatctg ccatttcttc tgtgctaggg ataatcaatg aaaaacggtt
    12841 cagctttttg atcaccccca tgtgatcatt cgctctagga ctttcttcca gcagcaacat
    12901 ttcacttcca gcttcttctg ttcctaaaat ggctaattcg ttttcttctc ttttcaaatc
    12961 aaagccaaca acgttcttgt aaaaatcaat cattttatct cggtctttca cccgaatggc
    13021 tacacttttt aaataggtgg cagcccccaa ttgaaaatct ttcatatttc ttgctcccct
    13081 ctacgtcaag ctttatttta acgatggatt cttcttcttt caagcaataa ctttgatttt
    13141 gttaaaaaaa atgtgagaaa attcgcattt tgcttgtttt ttgtaaatta atgagtaaaa
    13201 actcttataa aaagaggttg tgaaaaagct gttctgcatc aagtaataag aacagccaaa
    13261 caaaaatagc tcctcctatt tttcgtttgg ttgggcttat taccgcagat gcaagctttt
    13321 gaacaccgtt taaggaagag gttgtaaaat cagcggtttg acctgagtat tagagataca
    13381 aaacggaaaa atagcttctc atattttgcc gttttttgag ctaataccga aggacagctg
    13441 attgaacacc gtttattcgg aaaaagggac tgtgacacga cttttgtcac agcccctaaa
    13501 aaaaataata aatttgtact attctatgaa gcttcttctt cagaaaactg actgttataa
    13561 agatctgcat agaatccgtc tttttccatc aatgtatcgt ggtttcctgt ttctacgatc
    13621 gaaccatgat tcatcacaat aatgttgtct gcatcgcgta ttgtagacaa gcggtgagca
    13681 acaacgaaac ttgtccggtt ttccaatagg cggttcatcg ctttttgaat cagtatttct
    13741 gttcgtgtat ctacactaga tgttgcttca tcaaggatca ggacatctgg atttgctaag
    13801 aatgcccgcg caattgtgat tagctgacgc tgcccttgcg aaatattgct tgcatcctcg
    13861 tttaatacag tgttatatcc ttctggcaat ttacgaacaa aatcgtctac gtgagcagct
    13921 ttagcagcct caatgatttg ttcgtctgtt gcatcgtcgt tcccatattt gatattgtca
    13981 tagatacttc ctgtaaacag ccaagtatct tgcagtacca ttgaaaactg tgcgcgcaat
    14041 tcatcacgtg aaagatcacg ggtatctaca ccatcgtaac gaatactacc tccagaaaca
    14101 tcgtagaacc gttcaagaag gttgatcagt gtcgtcttcc ctgcaccagt cggacctaca
    14161 atagcgacca tctcaccagg tttgacatcc aagttaaaat cagtcattag caagttatct
    14221 ggtgtgtagc cgaattgaac attttcaaaa cgaactttgt atggtgtatc tttttcttct
    14281 ggaaggccag atttttcatt ggacatctct tcttcatcca acacttcaaa tacccgttcg
    14341 gctgaagcaa ctgttgattg gatagtgttc atcaaacttg caagttgagt gattggttga
    14401 gagaactgtg ttgtatattg caagaacgct tgaacatccc ctaatgtcat gttcccattt
    14461 gcaactttta ctccgcctag tacagcgacg aacacatagc ctagattttt aacgaaattc
    14521 atcaacggca tgatgattgc cgaaataaat tgagctttcc aaccagcggc atacagattc
    14581 tgattttctt tttcaaatac ttcctggtct ttttcagtat gattgaatgt tttaacaatg
    14641 gtatgaccac catatgtttc ttctacttgg ttattcaata aacctaaact tttttgctga
    14701 tctgcgaaaa acacttgtga tttcggcgct acgatcatca cgatgatcaa gcttaaaggg
    14761 atcatcaata acgcgactaa cgtcaattgc cagctgattg aaagcatcat cacgatgacc
    14821 ccaatcaatg tgactgcact agttacaaat tgagtcaaat tttgctgcag cgtgcttgcg
    14881 atgttatcca tatcattgat tgctcgagac atgatatccc catttgtatg tgtatcataa
    14941 taggaaatcg gtacacgatt cattttggct tccaaatcat gacgtaattc ataaactgtt
    15001 cgttgggaaa cacgtgtcat aataaattgc tgtaaaaagt tgaatacagc agaaatcaaa
    15061 tacatcacga ttactgtcgc aatgatctgc gcgattttat caaagtctat cggaaatttt
    15121 gttacttgtt ggccttgttg catctgttga gcgccagcca ttacaccttt gaatatttct
    15181 gtggttgctt tccccaagac ctttggtgtt tggatttgga agatcacggc tgctattgct
    15241 agtacaaata cagcgatgat cgcaaccata cgtttagaca tatagccaaa caatcggcgt
    15301 accgttcccc agaaattttt aggcttttca ccttttacac cgagatttct gccaggtcca
    15361 ccatgtcctg gaccgcctgc tggtccatga gagggacgtg tgtttgtttt actcatgcta
    15421 agtcctcctc tcttagttgt gaatgaacga tttcttgata agtttcgttt gttgctaata
    15481 gttcttcatg tgttcctttg ccaaccagtt ttccttcatc tagcacaagg atcatatctg
    15541 catccattac tgtacttact cgttgagcaa ctaaaacggt gatactttct ttcatattcg
    15601 ttttcaatgc ttggcgaaga ttggcatccg ttttgaaatc caacgctgag aaggaatcat
    15661 caaacacata gatatctgct gttttgacta atgcccgtgc aattgccaga cgctgtcttt
    15721 gtccaccaga gaagtttcct cctccttgtt ccacatggct atctaagcca tcttccatct
    15781 ctgaaacaaa atctttagct tgtgcgattt ctaatgcttt ccaaatcatt tcatctgttg
    15841 catccggctt accatactgc atattgtctc ggatcgttcc agagaacaga actgcttttt
    15901 gaggaacaaa gcccattaat tctcgcaaat tatattgagt catgtcacta atgtccgttc
    15961 catttatttg gatcgaacca gattcgatgt catataatcg agggatcaaa ttgactaagg
    16021 tcgttttacc tgaaccagtc ccccctatga ttgcgacgat ctcgcccgac ttggcttgga
    16081 aatcaatatc ttccaatgca agtttttctg ctccatgata tcgataattg acatgattga
    16141 aggccaatgt tgcgtttttt ccagcaaaag aaactgtttt aggattttca gggtcatgga
    16201 taccgatttt ttcatccaac acttcattaa tacggtcagc tgaagcttgt gcacgaggca
    16261 ccatgataaa aatagcggaa agcatcataa agctgatcaa gatctgcatc gcataagtca
    16321 taaatgcaat cagattaccg acttccaaag tcatatctgc tatgtagtgt ccaccaaacc
    16381 aagtgattgc tacgtttgta ccgctcatga tcaatgtcat cattggcatc atcaatgcga
    16441 cgatcgtatt aactttgatt gccgtatttg tataatcttt attcgctaaa tcaaaacgat
    16501 tttcttcaaa gcgtgtctta tcgaatgctc gaatcactct gacccctgtc aatccttctc
    16561 gaaataccag gtttaaacgg tcagttttct tttgcatact tttgaataat ggaacagcaa
    16621 agtacatgat tccgccgatc aaaataatca ttactgggat gacaactaag aagatttttg
    16681 tcaactgatg atctttttga taagcaagta tgctcgcgcc aatcaacgtg attggtgcat
    16741 tgatcatcat acgtaaaaac atttgtgtaa ccatttggat ttgagtcaca tcattggtcg
    16801 ttcttgtgat caaagaggcc gttccatatt tatcaaacgc atcttttgtc agactctctg
    16861 attttttata gacatccgta cgcaattgct tacccagttt ctgtgattca cgagtagcaa
    16921 aaaatgtatt tgcgatagca gctaagatac tgatcaaaga aaagccgatc atcacaaaac
    16981 ctgtatgcca aatataatcg acatctcctt gcgctacccc tttatcaata atattggagg
    17041 taagtgtcgg caaatataaa tctgccagta cttggatcac catgaaaact gcagctgcaa
    17101 ttgctgaagt caaggagatc cgttttacga tttttagcat ctagttccct tctttctatt
    17161 tatatactcc gtttttcagc caagaaagtt tcaaattgaa ttcactgatt gcttgctcta
    17221 ctgagtaatc ttcagatgtt gaaaataagc gatacgaatg cgcaattgtt gtaaatacag
    17281 tattcatcac taattggatc agtaattcca attcatgatc attttttact ttcagcttcg
    17341 tccgatcgat caattcaagc aactcttttg catcttgttt gtgttcttta tgtggatcgt
    17401 gaggcgtttt ttggcgactc gtatgaggag cgacttttct tgaggcacgg taatccatgt
    17461 tcataaacaa gttacgataa aaagaagcat tctcgccttc caaaatctca taaatcattt
    17521 tagaaaaata ggattcgaat cctttgaata gatcaccatc ggattctttc attagttgta
    17581 acatatcttt agagctgtca cgtctcattg tctcaaagta ataaaaataa agatcctctt
    17641 tatcttcaaa atactgatag aaactgcctc tagggatatc cgctaatttg acgatattag
    17701 caatcgaagc ttcttttaag ggtactctag aaaactcgat tctcgcagcc tcgattagtc
    17761 ttttttgttt ttctttaggc aaatggaaaa atgtctgtgt cggcatatgg atcctccttt
    17821 ctttcaagat gacacattgt catatgacgc tgtgtcatta taacacctcc tacctgtttt
    17881 gcaagacatt tgcatagttt ttttcattat catccgatac attaaaaaag tgataagagg
    17941 aatccttcca tttgaattaa tgagagttgc ccattaaaat agccacatac taaacaaagg
    18001 atgagataaa tggcccaatc aattagtgtc caagacttca ataaattgcc tctcgatgct
    18061 gacagcatcg ttttagatac aagaaatacc ttagattaca caaaaaacca cttaagccac
    18121 tcgctttcta tccccgctgc tatcttgcca agacaattaa aaaatctaga tccaaagaaa
    18181 ctttattata ttctaagcta tacaggcagg cgatcagaag cattagccga tcaacttact
    18241 gctaatggtt ttcgtgctgt ttatatcatt ggtgggatgg aagctcttca gtcgagtgtt
    18301 gcttgaatat tgcttgttag attcaaaaga ggctgtgaca aaaaatcgtg tcacagcctc
    18361 ttttataccc tgtctttttt tagttattta ttcttcaacc ttatcaatat gacttgatag
    18421 ccttttcttt ttctcatagt tataacaatc atagcaataa aaggagaagg taagcatatg
    18481 gcatctgttt tagtagtcaa aggtcatccc ctaactgcag aagaatcgcg cacagttaag
    18541 gcattgacgt cttttttaac aagttataaa gaaaatcatc cagatgatga agtgactgtt
    18601 ttggaacttt atcgcgatga tattccggaa atcgatgaag aattattatc aggttgggaa
    18661 gcattacgtg ctggtgcaga attcacttcc ttatcagaaa gtcaacaaca aaaaattgct
    18721 cgtttcaatg aattgactga tcagttcctt gctgctgata aaatcgttat cgcaaacgcc
    18781 ttatggaatc taaatatccc aacaaaatta aaagcttggt tcgataccgt caatgtagct
    18841 ggaaaaacgt tccgctatac agaaaacggg ccagaaggat tagtgacagg taaaaaagca
    18901 ctgcacatcc aatctaatgg cggtgtatac aacggacaag actttgcctc tcaatatgta
    18961 aaaggtattt taaactttgt aggcatcgat caagtagatc agctattcat cgaaggaatc
    19021 gattatgacc ctgaccgtgc agatgagttg atgcaaaatg cattagataa agctgctgct
    19081 ttaggtaaat cattttaaaa attgagataa atgcatacaa aagagacaga ggaaaaaatc
    19141 ttcaaagcag catttgtacc ttggatattt tcccatctgt ctctttttgt tattctttct
    19201 gctttttcaa cggattttct tcataaagag aagagattgt tgtgtaccat tcaaaaacat
    19261 gagtaatcac tactttagct gctgcataaa caggaatccc caagatcaca ccgatgattc
    19321 cgaacaattt cccagaagtc agcagaacta ataaaatcgt tactggatgg attgctaact
    19381 gattccctag tactaatgga gagatcaagc gtccttctat tgtctgctct actgcaaaaa
    19441 cgatcagtac tttgatgatc atgaatggac ctgctacaat ggctaaaaag atagccggaa
    19501 tcatcgctaa aaatgatcct aagtaaggga tcaggttcaa aaaacccgca ataatcccca
    19561 acgtgacagc atactctaaa ccaatgaccg caaaaccaat cataaacatg attgccacag
    19621 caaaagccac agttagttgt cctcttatat aagaagaaac ttgactattc atttcactca
    19681 gcacattcaa cgtcggtttt tgcatgcgtg tcggtaaaaa tttgacaaaa taaggtgcta
    19741 attgtttacc gtctttcaat agataaaata agataaacgg catcgtcagc agagccacta
    19801 aaatagtcgc taccgcgccg acaaaacttc caagactctg aactgtactc ttagatatat
    19861 cttggatcat atcgcctgcc gaattcatga acttatcacc tgctgtttcc aactgttcgc
    19921 gaaattggtt gaacagcggg tcccgcaaaa tctcggttaa tttattatca atcgtatcta
    19981 catattgagg gaagttactg atgaagctca ctgtttgttc ctgaatctta gggatgatca
    20041 ctacgctccc ccatgcgatt agtgcaacaa ccaaaacaaa cagcccaatg atactgtaaa
    20101 gccttggtac ttttcgcttt tcaaagtaat caactaccgg attcatcaag taatacaaga
    20161 ttcctgctaa aatgataggc agacctacga ttgctaaaaa ttgccagatt ggtgcaaaca
    20221 aatatgaaac ttttgtaaat aaaaagacga tcagcaatat taacaataca acaagtaagg
    20281 ctgtgactac ttgattattt aaaaaccagc gccaaaacca cgataccggt ctgttcttct
    20341 tattttttcc ttccataatt cctcctgtta gttataaaaa atttggcttc ccaaaccaat
    20401 agcaggcagt ctatgcggat gaagatagat tccatttgca atttgtcctt cttctggaca
    20461 agcatcaaaa atcaatgata cgtgagctac tgtctctaaa ttcccattgg ccatcggacc
    20521 gacataatcg atcgtatata cctgatcatc aaaagcaatt gttccgcctg ctttcaaaga
    20581 aaaaggttcg tcttttgata cttcttgaat caccgaatat tttcgtaagg cagctgttgc
    20641 tgttttatcg aacaaaatca gcatgggttc ttccttgttt attgcttctg tcccaatttc
    20701 tgtaatcttt gcctgaatca ttgcactctc tcctttaatt gctttcaaaa acagtataac
    20761 acattctcat tctttagccg tgtggaataa ttatgtgata gaataaaaac gaaaactatt
    20821 tcattacaat taggaggaat cagctcatat gatcgaaaag ataattttag gaacctatac
    20881 gcgtcgcgaa agtaaaggaa tctatacgat cgaccttgat acagaaaaag aagaattagc
    20941 caacctttca ttagctgttg aagaaaatag ccctacctat gtagcaaaaa gtaaagctgg
    21001 caatctttac acagttacta gtgtggatgg attaggtggt gccggtgcgt acgataagga
    21061 ttttcatttt ttgaatgcgg taacagagtc tggcgctcct ttgtgttatg tagcggttga
    21121 tgaagcacgt caattactat acggggcgaa ctatcataaa ggtgaagtaa atgtctacca
    21181 tatattagga gatggcgaac tgaaagcagc agattcaata ttccatcaag aagcgacagg
    21241 tcctcataaa aaccaagatc atgcgcatgt ccactatacg gatctgactc ctgaccaacg
    21301 attagtcgta tgtgatcttg gtacagatcg tgtgtatact tatgatgtgt ccgaaaacgg
    21361 caaattgaat cttgtaacta ccttcactgc agagccagga acaggccctc gccatcttgt
    21421 ctttcatcca aatggacaat tcgcttattt atttggtgaa ttagacagca cagtccgcgt
    21481 gctttcttat aataaagaaa caggtgcttt tgaagaatta cagaaaatat cgaccttgcc
    21541 agaagaattc gacggtgaaa atggaggggc agctatccgc atttcaaatg atggcaagtt
    21601 cctttatgct tctaaccgag gacataactc gattgctgta ttcgcagttt ttgaagatgg
    21661 ttcccacatt caaaacaagc aaatcatctc aacagaaggg gattttcccc gcgatttcgc
    21721 actagatcca agcaataatt ttgtcgtgtg tgccaatcag aacaccgata atctgacatt
    21781 atatcgccgt catcctgaaa caggattact ggaattactg aaaaaagatg tctatgtacc
    21841 agaatgtgtc tgtgtttatt ttgatcgtta gtaagaaaag gagaatgaaa tgaatcagca
    21901 agacgtatta gacagactac gggaagaact aaatattcct ttctttgatg ggaaaatcga
    21961 agataaagag tatagcgaag aggaatatca gaaactcaaa gccgatttac agaactactt
    22021 tgaacaatat gtacagaatg tagaaaacta atcagctttc catactatct gaaaagaaca
    22081 tcaggacaag acttttccgt ctatcctgat gttcttttct acaaataaaa aaccgccgag
    22141 atggttagac tcgacggaaa aggagtattt actcgatttt gagtaagtac aaggttgttg
    22201 ttggttatag gtatgtcatt atcataaagg attttgatca ctctatcata aaggtaatat
    22261 tacatttata ttagataact ctagttttta ttgttatcaa agatttcttt tattttttca
    22321 acaacaaatt agtcattttt tggatggcag ctgtcaattg gtctgtcgct tggtcttgtt
    22381 ttgcttcaga tgtattgaat agatcttcat ctacccctga ttcaagatcg atactttgga
    22441 tcatttcttc acagcctgct acatatgaaa gataagcttt ttcaaaattt ttatgaatgc
    22501 ccattgcttt tgctggtgga cgcaaagtac tgactttctt aagcattgcc cgatattttt
    22561 ttgttccttc ttcaaataac aggatgatct cttcaagagt cgatttgtcc agttcacttg
    22621 tttttccttc atcaattgcg tttctgatca cttcatattt cggatgcatt ccttctccga
    22681 ttgcttctgt ttccttgacg atttgatcga tggactgcac ataatagcca atattcggta
    22741 acatgttcgt tttcctactt tctccattgt tgttattatt tattctactt gtttttatcc
    22801 tcaaatgaca agtacttgaa aaaatttaat ggtttctata cagctcgcca ttcttattcg
    22861 tgctcaaaga aaccatctgc taaactatat tgtttccctg cgacaatgtc atattggatc
    22921 aattgatatt cgtcgtaaat ctcttgttgc gagcgattga attgcgcttc tttataccac
    22981 tgtccgtttt gatagtacag ttctcgttca aacgccggaa gttcctgttc taatgataaa
    23041 agcagctgat aaaagccatt tgttgtctga ttcgtctgtt ccattagatt cgctgcaaaa
    23101 taaaacggac tggtgattgc atcgtgagtc gtttgctttt ctaattctcc tttactgtcg
    23161 aacataagaa attgtgtttc atgcaatgtt tgtttgtcat tttttgcttg gatcgtatct
    23221 gaatagatcc ctggaagatg atccccccaa aaaacaacta aagtccttct agaaagattc
    23281 ttcaattctt ccgtgaactg cttcagtgcc gtgctgctat aggaaatatc ttgcagataa
    23341 ttctctaagt caagtgtccc acttccatct gaagtttttg caggatagtc tagattatca
    23401 tactttccat cgtagggcat atgtgtctgc atcgtgacta aatgaatgaa ctgaggtgtt
    23461 ttttcttcct tgagaagtgt cattacttct ttgtaagctg actcatcaga aatatatgga
    23521 ttattctcta tcgtatccgt ataggtcatc gtacgctcgc tgatgaactg gtcaaatcct
    23581 aatgtctgat aaacatcctc tcttttatac atcgaagtat tgtatggatg gatcgctgtc
    23641 gtctggtatg attgtgcgtt taacgctgaa acgatagaag gaagctgatc catttttggt
    23701 acaagcatcg tgtaaggggt ggtgagttgc gcattaaata acgccataga aaaaccagtc
    23761 aatgcttcaa attcgatatt tgctgttcca ccgccatagt tttgagaaag catattgcca
    23821 ctatatgttt gatcagctac ttcataataa tctgccaatg gctcaccaga tacttctacg
    23881 ccgtttaatc gactaggatc tgagaaactt tcactcatca cgaacacgat gttcggcgac
    23941 tcttcctcta cagttttatt tttttcatcg gctagcttct ggtatttttc agtgatttct
    24001 ttgatctttt ctttcgaata tccttcaggt tcatccattg gttctacctt cagattgtac
    24061 aaaaacccac cgataaaacc agtgttatag tagttcattt tctggctgta aggtatccat
    24121 agtgccgttt tattatatgc ttttcgcaac aaattatcgg gattgttgaa attactgata
    24181 taccctagca gtccaattgc cacaagcagt gtcgttaatc ggatcaattg gaatcgcctt
    24241 ccttttttca ggcttcgaaa catgtaccag caaaacaatc ctatcacggt tcctgcggca
    24301 gctaagatca ctgtaaacag catagtgccg gtcatatctt tcagcaatcc gatttcagtg
    24361 atcatcttca aatcgtctgg ataaatcggt tcttgccgat aactcatttt catataattt
    24421 gcaaaaccta atacaccgat ggctacgaca tacgtaaggg aacctaaagg gacagatcct
    24481 gctagtgcaa tcagaaaaat aagaataatc agcaagacga gacaggctaa gaagaatttt
    24541 tccgtatgcc aagaaaaagc aaatttcaac gcaaggtcta cggatagatc attttgacac
    24601 cactgcaaaa ataaattact aaaaacaaca agaaaaaaga tactgatgat tgctgctgtt
    24661 ccttttaaaa tctttttctg tttggtcgaa agtctgctta tatatctttt caaatgcttg
    24721 ctccttcatt tctatcaata aaagaatgtt gcttctctta ttttagttgt acgaaattcg
    24781 aaagtaaaga acggaaagaa gaattaatga aatccaaata ttctctttct ttatggcctt
    24841 tctggtttct ttaataagcg gacctttcgt actccagaaa ttttttctag ctgttgtttc
    24901 acttgattga gctgttcaaa atcagtactg tctatgtcaa tcaatatgta gacatgctca
    24961 ttcttatggt tacgcgatat atctgcgatc gctagctcgt tctcattcag cagagaaaat
    25021 atctctgcta atatattggt tttcttttga taaaatatag tgaatcgaaa cggcgcttgg
    25081 aaaaacattc gtgcggatgg ataattgacc gcttctctaa ccgtaccgaa caaaagataa
    25141 tcttttaagg cttcaactgc caaacgtccg ctgtctgcca aagcttcctg agtcgatccc
    25201 cctagatgag gcaacataag tatacggtca ttttctaaga actcttcttc tggaaaatca
    25261 gtcaagtatt tagccagatg ttgtttttcc aatgcttgca taacagcata tttatcaaca
    25321 atttcacttc ttcctacatt gataagtaca gcatcttttt tcattttttc tatattcgcc
    25381 tgatcgatca atccttttgt atcttctgtc aacggcagca tgactacgat gtaatccgag
    25441 gtagaaagaa ctgtttctaa atctgcttgc cgaacataat ccaactgggc atcgcggata
    25501 gaataaccca agacatccat ccctaagcta tagcaactta gcgctacttt tgtgcctatc
    25561 gctccaagac caagaagacc gatggttttg ccttgcaatt ctcgtcccac ataagcactt
    25621 cttttgtttt ctgcctgttc taaaatatta ggacctgtta gtgtttgtac catacgactt
    25681 gcttcgatta tcggacggga agataagagc aaacagcaca aaacaagctc ttttactgca
    25741 ttagcattta cacctggtgt attcatgacg atcgtcccgt tttcagaagc tttttcaacg
    25801 ttgatcgtgt tcaccccaac accagctctt gaaattgcta gcaaatctgg tgtaagccat
    25861 tcatctttca ctttcgtact acgaataact attccttcgg ctggcaaatc atctattatt
    25921 ttgaaatatt ccccttgtaa cttttctatc tcttgttctg gaatagcatc aatcaaatga
    25981 acgtgataca taaaaccccc tccttactaa caagtatctc gaaatttctg caattagaca
    26041 actgtttacc atttgacttc ctagaagcaa aaagagcagt tagagcagag taggctgagt
    26101 gataaaaata aggctatgac aagttactgc cataagcctt attttcgaga gcaaaatatt
    26161 gagggtgaga tatcttgata aatggtgttc cgttagctga cattgataat aagactaaat
    26221 ttcaaaaaag ttgaaaagca aatttcgtca atctcttctt atttcttaaa actaagtggc
    26281 tttttcacaa cctcttttta tctaataacc actgtttcca actcaactaa ttttgcccac
    26341 gtttcaattt gatctgttgt tagatttagc gagacaacgg tatgatgacc gccacctgcc
    26401 tgaatccatg aatggacacc ttcatggaaa ttcggtttag ttttccataa cacacgtgca
    26461 acaggtaaat taggtgcttc agtcgttggt tcaaaggctt ctacttcatt gatcagcagt
    26521 ttataatgcg taccgaaatc tgccatcgat acgactaccc cttctcctgc tttcccatca
    26581 aatacaagac gcgccggatc ttctctatct cccattgata aaggagaaac aacgattctt
    26641 ggtttcgtcg cagcaagcgt cggatctact tccatcatat gggattccaa gattgcttct
    26701 tggccactag ctagctcgta agtataatct tccatgaatc ctgtatcttt ccctcgagcc
    26761 atgattttca gcaaccgatc aatcgctgca gttttccagt cgccttcccc agcaaaaccg
    26821 tatccttctg ccatcaaacg ttgtacagct agtccaggta gttgtttcat tccatacagg
    26881 tcttcaaaat tagtcgtaaa agcgttatag cctccttctt ctaagaaacg gcgaataccg
    26941 atttcctgct gagcttgtac ttttacatgt tcctcccatg tcttttcttc ataatcacca
    27001 tagtcgaagg tatacaattc cttgtactct tcaaataatt gatcgacttc ttcgtctgat
    27061 acgcgatcga ttacttgtac aagatctccg ataccaaaat aatcaacagt ccaaccaaat
    27121 tgaatctgtg cttccacttt gtctccttct gtgacaccca cgtttctcat gttgtcacca
    27181 aaacgagcca cttttattcc aaaactttca ttataggcta ctgcaacatc catccaatca
    27241 gcaatctctt tttggatctc aggtcgtttc cagtagccaa cgacgatttt attttgttta
    27301 tttaatcgag cattgatgaa cccatattcg cggtcaccat gagcggattg atttaaattc
    27361 atgaaatcca tatcgatcgt tttccaagga atgctttcgt tatattgcgt agctaaatga
    27421 agcaatggtt tttgtagaag ctttgtcccg cggatccaca tttttgctgg tgagaaagta
    27481 tgcatccaag tgatcacacc tgctacttct tctcgataat tgacctcttt catgattttt
    27541 gtgatcgtat cagcagtaac tgctaattct tgaaggcgga ttggataagg caattggcca
    27601 ttttcattca gttcatcgac catttcttgt gcatgtttct ttacttctcg cagtgcttct
    27661 tccccatata aatgctgtga tcctacaaca aaccaaaatt ctttttctcc aatattcaac
    27721 ataattagcc actcctttta tttgttttgt ccgtaatacg cattttttcc gtgttttcgt
    27781 aaataatgtt tatctaaaat ccgttgaggc agctcttctg aaaatgaatt gagctgtctg
    27841 gtaaataaat tcatcttgca tacttcatct agtacgactg cgttcattac cgcactttgc
    27901 gcatctttcc cccaagtaaa tggaccgtgt ccgtgtaaaa gtacccctgg aattgctaag
    27961 ggatcaattt tgcgttcttt gaatgtttct atgataactt ttcctgtttc atattcgtac
    28021 ccgctgtcga tttcctgttg tgtcaaaaat cgagcacaag gtaccgatcc ataaaaagta
    28081 tctgcatgcg tcgtccccat cgcaggaaca tccaatcctg cttgtgccca gatcgtcgcc
    28141 catgtcgagt gagtatggac gattcctccg atttgaggaa atgaacgata taaaacagca
    28201 tgagtaggcg tatccgaaga gggattgagt ttaccttcaa tgatatgatt gtccaagtcc
    28261 acaacgacca tgtcgtctgc tgtcatctct tcataactga ctccgctagg tttgataacg
    28321 aaatatcgtg tttcaggatc aaaagcgctc acatttcccc atgtatattt gacgagtccg
    28381 tgtttaggca agtcaagatt tgcttggtat acttcttctt tcagttgttc cagcattttc
    28441 tatcactcct ttttcttgca attgatgaat cgctgttttt tcaatgtcga tcccctcacg
    28501 gtaacgtctc ataaatatct gatagcctgc tatttcttct gctgaaggtg tgatggtgat
    28561 tccttggtct tgtccaaaca cgtcttctgc caagaagttt tctaaagact tcccatgtcg
    28621 atccatcata taggctgcta ataacgcgat tccccacgct ccaccttctc cggctgtgtc
    28681 catcactgta actggtgcct ccatagcaga tgctagaatc cgctgtccaa cttctggtgt
    28741 tttaaaaatc cccccatgcc ccactaaacg atcaatttgg gcattctcag atttcaatat
    28801 atccattccg attctcattg caccaaaagc actggctaaa tggatccgca tgaaattcgc
    28861 taaatcaaaa cggctacctg gtgtacgtac aaataatggc cttccttcat ttactcctgt
    28921 aatgttttca ccggaaaaat aaccataaga aagtaatccc ccgcaatccg tatcgccttc
    28981 tagcgctttg ttgaataata ccgtgaataa tttatctctg cttatctcca tacccaaaga
    29041 attggtaaat tcttcaaaaa tactgaccca agcattgata tccgaagagc agttattcgt
    29101 atgaaccatg cctactagtt ctcctgaagg tgtagtgacc atgtcgattt ccggatggac
    29161 attttctagt tctttttcta atacgatcat agcgaaggcg gaagtaccag ccgaaatgtt
    29221 ccctgtccgt ttggctacac tgttcgtagc gaccataccg gttcctgcat ccccttctgg
    29281 cggacaaacc gggattcctg gtctcaaatt tcccgtaggg tcgatcagat aagcccctga
    29341 ttcgcttaac ttgccagctt cttctcctgc tactagaact ttaggcaaaa tgtgatccaa
    29401 gtcaatcgct acgtgcgctt tttcgattaa ctgaccaaaa atatttttca tttcttgatg
    29461 gtaggttttc gttgaaggat cgataggaaa catgcctgat gcatctccta cgcctagtac
    29521 tttctcacca gtaagctgcc aatggacata acctgcaagc gttgtcaaaa aattaatttc
    29581 tttcacatgt tcttctttat tcaaaataga ttgatacaaa tgagcgatac tccatctctg
    29641 cggaatattg tattgaaatt tttccgttaa tattcgttct gcttcttcag tgattgtatt
    29701 tcgccaagta cgaaatggta ctagtaactg ttccttgcta tcaaatgcta gataaccgtg
    29761 catcattgca ctgaacccaa tcgacccgat tgtcgtaaga ttctcgccat agatttcgaa
    29821 tacttctgct gccatttttc ggtaactgac gcgcagtcct ttccaaatat cttctaatga
    29881 ataggtccag ataccatttt ctagtttgtt ttcccactca taactaccag cggcaatagg
    29941 agtaaaagat gaatcaatca atacagcttt gatcctagtt gatccaaact caatccctaa
    30001 agatgtacga ccatctatga catctaacat actttgcgca tttacttcct tcataatgat
    30061 ttaactccct ccttctcgtc aaacctgatc aaaccgtctt tttagtagcg cttacaatga
    30121 ttattgtata cttttgtacg tacatgtcaa gcggttattt acaagaatac aataaaaaac
    30181 acttgttttg tacgtacata agttataaaa tgaaattgct tacaaaatct gatcaaccgc
    30241 cttctcgaaa ttctagaagg agagaatact tatgaaaaaa gaaaaaaaag tttcctcatc
    30301 gtttatttat ttttttggcg catttggcgg cattctattt ggatatgata ttggtgttat
    30361 gaccggcgcc ttaccttttt tacagcatga ttggggtttg gcaggtaaag cttctttgat
    30421 aggatggatc acgtcttcgg tcatgttagg tgcgatcctt ggcggctctt tatctggtct
    30481 tctttctgat aagcttggac gacgaaaaat gattcttctt tctgcactta tttttatggc
    30541 tggttctgtc ctttctgcat ctgcccctca caatggttct tattttctga ttgcagcaag
    30601 gattcttcta ggtttagctg ttggcgctgc atctgcccta gttcctgctt atatgtctga
    30661 aatggctcct gctagactaa ggggaagatt gtcagggatc aatcaagtga tgattgcttc
    30721 tggcatgctc ctttcctatg ttgcagatta tttgctaaaa gtgttgcctg aaacgatggc
    30781 ttggcgcgtg atgttagggc tcgctgctgt ccccgcgctg attttatttt tcggtgtttt
    30841 agctctacca gagtcaccgc gttttcttat gcaatctggc cgcttggaag aggcaaaaag
    30901 agtattgaac tatattcgga caccaaaaga agctgaacag gaattcgaac aaatccagct
    30961 gaatgtcaaa caagaaaaga caaccggaac ttcttggcat actttgtttt tagaaaaata
    31021 tcgttcctta gtcattgccg gtattggtgt agcagtattc cagcaatttc aaggagctaa
    31081 tgctattttt tactatattc cgctgatcgt agaaaaagcg actgggcatg cagcgagcga
    31141 tgcattgatg tggccgatca tccaaggaat cattttagtt gctggttctt tgctcttttt
    31201 ggttattgca gacaagttca atcggcgaac tttattgaaa ataggcggta gcgtgatggg
    31261 actttcattc atcctaccag ctgttttagg aactgtactg gatgctcata caaattcgtt
    31321 gttgattctc ttgtttttat gtatttatgt cgcattttat tcttgcacat gggctccact
    31381 gacatgggta atcataggcg aaatattccc actagctgtc cgcgggcgag catctggcct
    31441 agcctcttct tttaattgga ttggttcttt cctagttggc ttgctttttc ctgtgatgac
    31501 tgcttctatg tctcaagaaa tcgttttcgg catttttggg atcatctgct ttttaggggt
    31561 tttatttatt caagagatcg taccagaaac tcgtgggaaa tctttagagg aaatcgaaca
    31621 aagtgcatct aaaaaaactt atcctaaacg tatttctatc catcattctt aaagaggttg
    31681 tgacagaagt ggtcagcttc aagaaataag aaggaaccgc ttagtttcca gtcacaagga
    31741 acaatttcaa aaaacagctt ctcatgtttg atgaatattg tgacttgtga ccagttggta
    31801 ggagaacacc gtttagtgga gaggtggtga aagaaacgtt cagccttgag cgatgacaaa
    31861 agctctcaca aacattgcga cgagtaaccg taggagcaat aagaggcggt aaaaacccgt
    31921 ctcttatttt tcgttttctt tcactgtttc ccgttcaact agtacaggtt catagagaat
    31981 atttggctgt gcctgtcctt tttcaatcgt tgtgacgatc caccgtgcgg catcttgtcc
    32041 caacttttct tttggatgcg tcaatgttgt caatttttta ttccctaaat ggcttaaaga
    32101 agaatcgtct gttcctacga tcgataggtc atctggaata tgatagccat tttctgtcaa
    32161 cttatctagc attaaactag ctatttgatc gttatagcaa atgatgccat ctaaagcttg
    32221 ttcttctttc aaccgttgta ctgttttttc agcgattgtt tctcgtgttt ccgtggtgaa
    32281 agtaatgata tcttccgtca aaaaacgcac attatactgt tcacacgcgc tgataaaacc
    32341 tttcatcctg tattttcctt gaagatcgtc gatttttgtg atgaacaaca gacgctgatg
    32401 tccttttttt atcaaatatt ctgctgccat aaaaccgact ttcacatcat ctaaacaaat
    32461 ataagaggca tttaactctt cataagcagc attgatcatc accatcggaa tgccttgttc
    32521 tctcaaagca acataaagtg ctaagtttgg gttatactga ttacttttcg taggctcaac
    32581 gatcaaacca tctaccccat aagcaatcat tttcttcaaa cactctttct ccaattcgtg
    32641 atcgttattc gtactggcta aaagcaatga atacccagat tctcgtaatt cattttcgat
    32701 tcctcggata atcgacggaa aaatataatc agagagatat gtggtgatga caccgatcgt
    32761 ttttgttctg ttcaatggtg ttttttcttt cttatagctg tcatctacat atgtgcccga
    32821 ccctttttct ttacgcagat agccctcatt taccaacaca gcgattgctt gccgaatcgt
    32881 atgtctgctg acgtcatata tttcttgcag cttgaattct gagggaatgg ttgtatttac
    32941 ttcgtattcc tcacgaagga tcttttgttt tatctcttct gcaatatctt gatattttgc
    33001 catttgcctt gccccttttt atttgtacgt acatatatca taacgagtct gcaaggttta
    33061 ttcaagcaaa aatcaaggtt tcttgtgctc ataaggcata cggaacaggt tcaccaaatt
    33121 ctggaaatcc ttgctcattc cacgtaaagc attgcgcgtt cgcgtgccga ttaggattgt
    33181 ctaacggatc ccctttttgg ttcttctctg gtcgagcatg atagatcaaa atatcaaagc
    33241 acccgtcttc tgaaaccgta aacgagttgt gaccaggacc aaattgctta tttttttcag
    33301 aagaaacgaa tactggctcc gataatttat gccatgattc ttttaataaa agattcttgt
    33361 gctcatcagc ccacagcagg cccatggcat agttttcatc tgttgcacta ccagaatacg
    33421 taatgaatat tttcccattg cgagtaagga ctgctggacc ttcatttact gaaaaacccc
    33481 gtttttccca ttcatactca ggtatagaaa ggaggatctg cttccccttt aacgtccaag
    33541 gattatccat ttcggataga taaagattag aattcccagt aatttcagga tcttgttggg
    33601 cccacacata atatagcttt ccttgagaag agaaagttgt tccgtctaaa ctgaaagttt
    33661 cccttttcgt ttttatttgt cccttttcta tccaatctgt attcattgga tctatatgct
    33721 gattttcaag tacgaacatg cggtgatgat gagattcatc acgtatagtt ttatgattgc
    33781 ttgcagcaaa gtaaatatac cattttccat tgatataatg gacttcaggt gcccagatca
    33841 attcactcat cggtccttct tcatgagcct gccatacaat caggcttgca gcatgttcta
    33901 attcattcag tgactttgcc cttcttaatt caatcacttg gtagcctgga accgatcctg
    33961 taaaataata ataaccatct gtatgtttat atacccaagg atctgcccgt tgccgaacga
    34021 taggattatt ataaagtgtc ttcatcataa caaatttcct cacattttct ttattctaat
    34081 tacattccaa gagtattttt ctatccctac atttaattcg tcatgttttt ctattgaaaa
    34141 atagtttatt tcaggtttta ttttttctgg ttcttcacgt gtatttttgt ccaacagatc
    34201 gctactggtt aatgtgtagt gctctccttg atctagcaaa ttgaaatcat gttcaatcgt
    34261 tatcttttgt tgttcatttg tgcgattgat caagaaaata gtcagctgat tgccgttatc
    34321 tacgatcaca ctgtccacat aaggaacatt ttttatcttt tcacaatcat aaacagggca
    34381 aatgatttgt tgtttcaata cttgaccttt agcatatttc gataagtgtt gcatcacata
    34441 ataagttgga ttcctccatg cttgtccttt agcatctgtc agtattaaag gtattgtgtt
    34501 tactaacaat gattgacaac taatctttac gcgatctgcg tgtcgtaaaa tagccagccc
    34561 taatgaacca aataataatg tatcttccat agtaaagtaa caccaatcga tgggactacc
    34621 gacttcaaaa tccctatgtc ttttttccgg ttgggcatgt acattccatt catcaaacgc
    34681 tagatacatt gttttctcac ttctcaaata tccttttaca taatcacaag tagcaacgat
    34741 ttcttcaatt tgacgatcga atcgataagc ttttgcaagg tatgtcgctg tatcatcacg
    34801 ctcattttta ggaggtttag tcaagtcttc ttcatcatac ttatcaatat agttatgcaa
    34861 agctaaataa tctacattct cataagcttc ttctaatgcg atacgatccc attcagggta
    34921 cgatggtaaa cgtggcgtag agcttccaac taagattgtt tcaattgaat catccactaa
    34981 cttcatgact tttgaggctt catttgctaa cctaccatat tcataagcac tcttagcccc
    35041 cacttgccat ggaccgtcta gctcattccc taaacaccat aatttaacgc caaaaggctt
    35101 tttttgccca ttttttattc ttaaatcact ccagaaggtt ccttttggaa aattacaata
    35161 ttctactaaa ttttttgctt catcaattcc ttttgttcct aaattcaccg tgaatatcgg
    35221 tttcgcttgt acatgactga tccatttaaa aaattcgtgg agtccaaaag aatttggttc
    35281 tatacctcgc catgctaaat caattttttt tggtcgttgt tctttcctac ctacagtatc
    35341 cacccaatca taaccagatg taaagtttcc tccaggatag cgaatgacac ctaattctaa
    35401 ttcctttaca taagagagga catcttgtct aaacccgtca ccatcagctg tttcatggcc
    35461 cggttcataa atccctgtat agacgacact tcccatatgc tcaacaaatg agccaaacag
    35521 ccgagaactg atctctccta ccgtaaaatc ctctctgatt tttactttca cttcttcgtc
    35581 caacagcttt ccctaccttt catttcctat agtcaagata atagatacaa gtttccatac
    35641 gccaccagca aagataaaaa taaaaggagg gaaaagaaat atgagcagta caaatcctaa
    35701 taacaatacg caacaaagaa tatttctcaa aaaatcaaaa aatactttcc atgcactttg
    35761 ttcaaaaatt tgtaaaaatg tgatatcttt gttttcagaa atcacatata tccaggataa
    35821 aagaaacatg gaataaataa gtaaaactac atatccacta attgaaagta cgattgtctg
    35881 ttcttctaat aattgattaa caagaaaata aataatgatc gttaacagac taaggaacaa
    35941 gttcttacca aaatgttgct ttattccttg aaagaaaaaa taaatgatat ttcctgatcc
    36001 ctcacctttc cattgcgcca ttacataaaa aacagcacat gtagcaggca cgatagtaat
    36061 aacaggcaaa cacacgatta cccagagaat acctaataag atgtaatcag aaaccacaat
    36121 caactgtcca taaaatttag tctcaactat tttcatcttc ctcactcttt cattccagac
    36181 atagctgttc cttgaacaaa atatttctgt gcaaaaatat aaaccactag taatggcagc
    36241 attgaaataa ccgttccagc cattacaggt ccataaaaag aaatgttctg ccctgtaaat
    36301 aaagctaacc ctgctggtaa agttctcatt tttgaactac ttgtcaatat caatggataa
    36361 agcaagtcat tccaactatt catcagcgtg aaaatcccta atgataaaag agtaggtttg
    36421 ctcaatggca acatgatttt caaaaaaata ttaaactcac ttaatccatc aatgcgtgct
    36481 gcctcttcca aagaatctgg taaagtaata aaaaaggaac gcatcatgaa gataccaaat
    36541 gcagttgtca tacgaggaat gatcaaacct gcataagtat caagcatgcc aagcaaattg
    36601 acttcaataa atagaggaat catgaagact tggaatggga tcatcattgt cagcaaaacg
    36661 aaatagaaca aaactgattt tcctttgaaa cgcatacgag caaatgcata ccccgcaaga
    36721 gaatcaaaga atacagaagt gatcactgtc cctccagcaa aaatcactgt atttttgatg
    36781 taatctaata aagggatcgt tgtccaaacc ttaatatagt tatctagggt atagctatct
    36841 gcaaatattc taggcggaaa tgagataatg tctttttcgt gtttaaaaga agaaataatc
    36901 agccacaaaa aaggaaacgc aataaccaac aatataacta gcatactaat cgttcgaatg
    36961 atttttttac cactaactgc ttttcgacta ttcacagtaa ctgtaatcga gctttctggt
    37021 gccatacagg tttccctcct atttcatttg tttttctttc cgtgacatgt acacattagt
    37081 ggagatagct atagttgcaa caatgataaa caaaaccgaa gacagtgctg atgcatagcc
    37141 caattcataa ggtgctgtaa atcctcgact gtaaatatat tgaacagctg tttctgtctt
    37201 aaattgtggg ccaccagcgg ttgcaacata tacttggtca aacacttgca tcgaaccaat
    37261 cagattcgtt aaaatacaaa aacctaagga gggaacgatc tgaggtagcg taatgttaaa
    37321 aaactgctgt cgatttgttg caccatccat ttgagcagat tcatacaaag agggagagat
    37381 tccctgaata gcagtcaaga gaatcgtcat agtaacacca aaattcttcc aaacagtcat
    37441 gatggctacc gtcggcatag caagggttgg atcgcgaaaa aattgaggga tcggcaagcc
    37501 gacttgtgaa aacaagtaag gaatcgcccc tacattcgga tcaagcaaca tagaaaaaat
    37561 aatcgcaatt gacgtcaatg aacaaataac tggaatgtaa aatgtcgaac gacaaaaacg
    37621 attgaatctg gtatttttag ataatgctac tgctgctaat aaaccaacga taatttgtgt
    37681 tggcgtttcg agtacggtga aatataaaga atgaatcaac gaattaactg ctcgttcatc
    37741 actaaatata cgtgtgaaat tttcaaaact tacaaaattt cttgatgtaa agaaaatatt
    37801 caaatctgta aaactaatag caaatgttcc taaaagcggt atgacagtaa ataataataa
    37861 aactaaaaaa gatggagtta aaaacaaata cgcagcacga tcaggacgat tccaatattt
    37921 ggtgaatccg ttcatatagt ccacctcatc tttcattttg ataaaatata caatcttggt
    37981 ttaaacgaat ctgaataaga tatccgaaca atcgctgtga tttttgcaat aacacctatc
    38041 tgcaaatact aggtcgatca ttcagatatc tgtatcttgc caacagtcta tcctgtataa
    38101 agttctgtac tatttttgca gtaattgatt gatggcatca tctgcatcgt tcatgagtgt
    38161 ctcaggatct tctcctgcta ataatttttc aatcagtgga ttgatgatat cgttattgat
    38221 cgcagagatt tgggtaaaac ctggtaaata tggctctgca tagtccattt gtttcgataa
    38281 ttcagataca ataggatcat tttttacttc ttcatctttt gctactgaat ccaagtaggc
    38341 aggaaaacca ttttcgatac tccatttttt accaatttca gttgtattcc aatatttcaa
    38401 aaactcatag acagcttctt ttttattatt atctgtactt gccggaatgg catatcctac
    38461 gccatctaaa atagctgttt ttttgtctga atcactccct tgtggtaagg tagtcacatt
    38521 ataatcaatc tcgtttgctt tcaatccgtt atttaaccat ggcccattga tttcaatggc
    38581 tagttgacca gcattcatta aattgtccat ttcggcgcca gaaatcgatt ctggaccagt
    38641 cttgtctaca tggatcattt tttggatcgc ttttaacgtt ttaaggttct caggagacgc
    38701 aaattcagac ttagtataat catcgttcac taatttaccg ccatttgcta acatccagtt
    38761 atataacggt gcagttccat ccttattgaa gacaaaacct gatacatttt tactactatc
    38821 agttaatttt ggtgccattt ctgtcaattc ttcccaagta gtaggtggtt gttcagtatc
    38881 taatccagct tgtttaaaca aatcattatt ccaatacata tacatgcctt gtacctgcat
    38941 ggggacaaaa tactgtttac cttctacttc acctaattca atagccgttt ctttaaaatc
    39001 agatttatct actccatcat attcccagaa atcgtctaat ggttgtaccg ctccgttttc
    39061 tacatactgt gcaaaatctg gataatttaa tgcaataaag tcaggtgctt ttttagaaga
    39121 aatcgctgta ggaagttttt cattcaaatt ggcccaagtc atgatatcca tttcaatttt
    39181 gatgttttta tcatttgttt tattaaattc attaacgatt tcttgcaata tttcaccatc
    39241 agatgcagta aacccattcc aaaaagtcaa agtcacttct ttatcagaat cagattctgc
    39301 tgaacttccg ccaccgcaag ctcctaaaat acctgttgca gctaccaaca aagagccaat
    39361 taaccatttt ttacctttca ttttttgttc ctccttcatc tgataaacga catctgacca
    39421 cattccaaga tagcggtttc aataaacaat tgattttccc atcttcagct tttacatttg
    39481 ttgaatgact tggttttact tgttcacttt taggaagatt agttgctttt gtatcaaatc
    39541 cactcatttc agaaaaatca atcactccct ctaatgataa ttcagacaag tgaacagtta
    39601 gattcatttc ttcttctcca cggttaactg caaatatgac aagttctttg tcatttatta
    39661 cggctatact ttctaaatac ggcacttctt caaaatcttc cgtcgcatag tttgctgaac
    39721 gaatttgtgg gcttaacacc tctccttgtc catacacagc agcttgcata aatggataat
    39781 agatcgtctg tctccaagct tctccgtttg tttctgtcct gattggtgca ataacgttta
    39841 ccaattgagc aagacaagca atctttactc tatctgcatg tttcaataaa gtgattaata
    39901 gacagcctaa tagtaatgca tcttcaaaat tatagttatc ttctagtaaa ggaggagcaa
    39961 tctgccaagg cgctgcttgc tcatcttgtt ccgctgaatg ataccaaata ttccattcat
    40021 caaaggacag gttgatctgc ttcttactat gtttcttcgc cttgactgta tcgcacatcg
    40081 ctgtcacttc agaaatgaac cgatccatgt ctaaggaacg tgcaagataa tgagaaagat
    40141 catttttttg gttgccataa tattgatgca gagacaaata atctatttga tcgtacgctt
    40201 gatcaagaac tgttaactcc caatcaccaa atgtaggcat ttgccgattc gaacttccgc
    40261 ataaaaccac ctcaattgaa tcatctacga gtttcatcgc cttagccgtt tcctctgcta
    40321 accgtccata ttcttccgct gttttatgtc caatctgcca aggaccgtcc atttcattcc
    40381 ctaaacacca tgtcttaatt ccaaaagggc tctcttgacc attcgaacgc cttaaatcac
    40441 tccaataagt tcctttagga aaattacaat attcgactaa attacgagct gcatctactc
    40501 cacgagtacc cagattgaca gccatattga tttcggcccc cacatgctga caccagttat
    40561 aaaattcatg aatcccaact tcattcgttt caattgtccg ccaagctaaa tccaaccttc
    40621 taggtctatt tgctatgggc ccaatactgt cttcccaacg aaatcctgac acaaaatttc
    40681 caccaggata tctaatcatg ggaatgttca attcttttac caagtgaatg acgtcttggc
    40741 gaaatccact tttattagct gatggatgat ctggctgata aatcccttca tatactgctc
    40801 gtcccatatg ttcaataaat gaactgtaca accgcggatc aactttgcct acacgcttga
    40861 cactactaat caacattgtg gcttccataa ctgacctcct ttaatttata catgaataat
    40921 acacctcgtt gaaagcgttg tcaataatat aaatgttaaa cttacttatt ttttatcgat
    40981 tttaattaac caaaatatta ataatgatta aaacaaataa tttaatataa cttttattaa
    41041 caaatatgtt aatatataag aaaaaccttt taagaggtga tatatatgca actacaaata
    41101 aacgaagaat ccttaccggt atatgaagca ttagctagta aaacaaggat caagatcatc
    41161 cagctacttt ccaaaaaaaa gatgaatgtc aaagatttgg caaaagaact tggagtgagc
    41221 agtgctatca cgacgatgca cgtgaaaaag ctagaggaag caaacatcat caaaacagaa
    41281 aaagtaggac aacaaaaaat ttcgagcctt cgtgtcgata aaatcgatat tagttttcca
    41341 gaaaaaatat tcaatgcttt tgatacgaaa gaaacatcga tacctattgg tcactataca
    41401 aattatgcaa ttgaaccaac atgcggttta gctacgatcc atgattttat cgggaaagtg
    41461 gatgaaccac gctattttat ggatcctcga aggatggatg cccgcattct ctggtttacc
    41521 agcggatttg tcgagtatca ggcacctaat tttttaaata cagaggatac attggagatg
    41581 ttggaagttt ctgtggaaat ttcttctgaa tttccttttt caaatgacaa ttggccttca
    41641 gatatcactt tcagcctaaa tggtgtggag ctaggaacat ggacgagccc tggagatttt
    41701 gctgatatcc gcggtaaata tacgcctgat tggtatccag ataatcttaa tcaatacggc
    41761 ttgctgaaga cgatccgaat taccaaacac ttgacgaata tgaatggaga acccttgtca
    41821 aacatcacga tcaacgacat ccctaaagag caagacactt ggcatttgcg aatcgaagtg
    41881 aaagacgatg ccaaacatgt ggggggctgt actttatttg gtaaaggatt cggtaattac
    41941 gatcaggata tcaaattaaa agtttactac agctgatatt tttcttttct attttataaa
    42001 acaaaaacag ccaatcccta tgaagggacg gctgtttttg ctttattgtt atttccagaa
    42061 atcatccaaa acggaaattg gcaaatgtcg tttatgttgt gtcttgttcc accatgcttc
    42121 gattttttct tgtgcttgag cgggaacttc ttttccttct agataatcat caatttcatt
    42181 atatgtgaca cctagtgcga cttcatctgc aatcattggt ttcccatctt caagatctgc
    42241 agtcggaatt tttgtgtata atttttccgg tgcattcaat gcttgaagca attgtttccc
    42301 ttgtcgtttg tccaaacgga ataatggcaa aatgtcggct ccaccatcac caaatttcgt
    42361 gaagaatcca gtaatgtttt ctgcagcatg atctgtccct aatacggctc ctgctctttc
    42421 tccagctacg gcatattgcg taatcatacg ttgacgtgct ttgatatttc ctttgttaaa
    42481 atcgctgatt cccacacctg cttcgcttaa tacttgtact tgtgcatcaa cagctggttt
    42541 gatatttact cgcagactaa catcgggctg gataaagtca agagcagctt ttgcatcttc
    42601 ttcatctgct tgttcaccat aaggaagacg aacagcgata aattgatagt tttcatcttt
    42661 tgtttcttca cgcatttctt ccatcgttag ttgcgccaaa cggcctgcta aagtagaatc
    42721 ttggccgccg ctgataccta gtacaaaagt tttaagaaac ggatattttt tcaaatatgc
    42781 tttcataaag tcaatacttt tacgtatttc ttcttttgga tcaattgttg gtttgacacc
    42841 taattcattg ataatttctg tctgcaggct catttattct cctcctatgc ttctgtatga
    42901 agttctgcta cgcttttttt cattcgttcc atgatagcca ttttatggtt ccagcattca
    42961 gtagacagat cgactgggta tttttgcgga ttcaagtctc gtttatactc ttcccatagt
    43021 gaatctagat tttcttttgt atattctttg atttcgttta gtgcaggtaa ttcgtaaact
    43081 ctttttcctt cgataaagat atcttgtaaa actgggcgag ctgtaaagtc tcgaacagtt
    43141 ttattgataa acgtatgcac tggatggaac atgaagatag cttcttcttc tcgagggtct
    43201 tcatcccata atgtcacgta gtctccttca gatttcccat caaaatttcg agtgatgcgc
    43261 cacacttgtt ttttccctgg agtagttact ttttctgcat tgcttgataa cttgatcgta
    43321 tccatcattt ttccttcatc atcttcgatc gatactagct tgaatactgc accaagggct
    43381 ggctgatcat aggctgtgat caattttgtc ccaacacccc atacatcaat tttagctttt
    43441 tgcatcttta aattcaaaat cgttgcttca tccaagtcat tcgaagcata gatttttgct
    43501 tccgtaaatc cggctgcatc taattgttcc cgaacacgct tagatatata ggccatatct
    43561 ccactgtcga tgcgtacgcc ttggaagttg attttgtcac ctaattcttt cgccacgcga
    43621 atcgcacttg gcacacctga tttcaacgta tcataagtgt cgactaagaa cacgcagtct
    43681 ttatgggttt tagcgtaggc cataaatgct tcataatcat ttccataaga ttggactaac
    43741 gaatgagcat gggtcccgct agctggtatc ccaaatatct tcccagcacg tacattgctt
    43801 gttgcatctg ctccgccgat atatgccgct ctagttcccc agactgctgc atccagttct
    43861 tgtgcccgtc tagttccaaa ttccaatagt ggatcttctc cgatgacaga tttgatacga
    43921 gctgcttttg tcgcgatcaa cgtttggaaa ttgaccatat tcaacagtgc tgtttctact
    43981 aattggcagt gcgctaaagg cccttccact tggatcaatg gttcatttgc aaaaactagt
    44041 tctccttcac gagcagaacg aactgttgct ttaaattcaa aattttgtaa atactccaaa
    44101 aaaccttcag gatagacttc taatccacgc aagtaagcaa tatcggaatc attgaacgtc
    44161 agattctcta aataattgac taaccgttcc aatcctgcaa aaaccgcgta tccgtggtta
    44221 aaaggcattt ctcgaaagta acattcaaaa acagcatgta aatcagctcg acctaattcc
    44281 cagtatgtct gcatcatatt gatttgatat agatccgtat gcagcgtcaa actgtcgtct
    44341 gggtaagtat tattcatctt gtattactcc tcttggcaag ttttcatttc ctacccatta
    44401 taacaaaaaa aattaatagg aaaagaaatc ttccattttt tctgaaaaag cacatgtttt
    44461 acggatattt cttgctaaaa atgaactcca tctgctaaaa ctatcctttc ttattggtat
    44521 ttaacctaaa cgagaaagaa tacttatttt attcaatgta tccgagacac gaagaaatta
    44581 gcgttttcta tttttcttac ttgataaaat aagtagattt tcctactaca atcgtagccg
    44641 ttaagatacc gatgattgaa caaatataac caaatgtctc tacaacataa tcaatggcaa
    44701 aagtttttga agcaataata cgtctttcct tttttgcttc ttatccccaa agaaacggat
    44761 ataacttatt atgtattgaa tttttttgga aaggcgaatt atgacaagat ataccttgcg
    44821 ttgaaaaaac tcgcggaaaa aatatcacac gtttttaaaa agatacaaag taagaaagca
    44881 gctgtcaata gaatggaaac aaaaatggcg agacatcaat cagtctcgcc ttgtttttta
    44941 ttgtatacat tagatatgga gcaggttacc ataaagatga ttattagtcg ttattaaata
    45001 gaatccttcg tatttttttg ttctatcact acaatatctt tatttggacg tttgaattgt
    45061 attccggcac gttctagctc ctgtttcact cctacatgga ctttgacatc ttctcgtgtc
    45121 atttttaaaa cgtgatgaat cgcatagtcg atttcttgcc ttgtgatttt tttcttcgta
    45181 ttgatcatca cgacttgctt tttatcatat gtgtctacca atagcagttg tgttcgatct
    45241 aacgaaaata atttgtaata attaatagcc gtattcccaa aaaagaattc cactacttta
    45301 ggataaaatg tcttaatatt taaattacta ttcacaggtg tttcgattag cttcatctca
    45361 ttcctctttt ctatatagtc gatttagatc caatcttaag ttatgttacc atctttccta
    45421 gcattaagga atcggttaca attgaacctt taaagacaaa aggatttacc ttttatcact
    45481 taatagtata acataatttc atgagaaaaa aatacaattt tttcttgacc atctcgttac
    45541 gtaaaggatt atgcttacat ttgtcaggag gaaaaataat ggagtacaca ataaagaaaa
    45601 tgtcagagat atcaggagtc agtccgagaa ccttgcgctt ttatgatgag attggactgc
    45661 taaaaccagc taggatcaat tcttcaggtt atcggattta tggcaaaaaa gaagtcgatc
    45721 gtctgcaaca catcttattt tatcgaacaa tggcattcaa gttggatgat atccaagagg
    45781 tcttagacaa tccatctttt gaccaccaga aagctttaat caagcatcag caaatgttgc
    45841 tggaaaaacg ggcacaaatc gataccctct tgacaactgt ccaacagaca cttgatatgt
    45901 acgaaggagg aagaaaaatg agtgacaaag aaaaatttga agggtttaaa caacaaaagt
    45961 tgaaagaaaa tgaagaatcc tatggacaag agattcgaca aaaatacgga aaaaaaaagg
    46021 ttacagcatc caatgaaaaa tggatgaata tggataaaga aacgtatgaa gaaatgcaaa
    46081 ctgttgaaaa gcagatgtta tttgatctca ctctttactt aaaagaacca gaagatgaaa
    46141 gcttagcaga ccgtatcttc caagctcata agaagtggtt atcttacagc tggcctgaat
    46201 atcagccaaa agcgcataaa ggactaggaa tgatgtacga agccgatgaa cggttcactg
    46261 catattatga cgagcgaagt ggtcatggtg cagcaaaagc tttaaatgaa attatccaaa
    46321 aaaaggcgta atcttttaca tgatccaaca aaaaagtaca cgtaaaatga cctaataaca
    46381 aaacagcaga cacccaagtt agatttttat gctaactttt ggtgtctgct ccattctttc
    46441 acaagttttt tcatcctact ttaaatatat tcatcgttga ataaacgtat aaggtatttt
    46501 cactttatcc tttttttctg taagaaacaa ttgaaatgcc atttctccgc aagcttccag
    46561 atggtggtcg atggttggga aaccaagaag ctcgctgatc agcaaattat cgcgtccaat
    46621 caccactggt gtcttatctt gcagatagct ttgcaagatg cctgctgcaa ccatatccga
    46681 atttgtaaag acaccatcaa ctccaagctc tttaaaaaag aagcctgctt gttttccttt
    46741 ctctgcatct atacaatccc aaaaaacaag tgattcatca aaagatggaa aaagttgatg
    46801 acatagctgc aaagtgattt ttgagttata acttagcctg ccacttcgcc ccaacgtgat
    46861 gcccaattgc tttatacctt gctgcttcaa ataagtaaga ccttctgtaa ttgattgttc
    46921 tcgatcaata aagacacagc ttgcctgttt tcccttgatt tcttcacaaa aaactagagg
    46981 tccatatttt tgatatgcca ataaatcatc tagtgtattg gcacgagacg taatgatcaa
    47041 tccatcgaaa gctttagctg caaattcttc aagatacctt cgctcttttt ctttctgata
    47101 gcccgtcggt aaaagtgtca ctttgtaatc ataatcaaac gctgcttcaa tgatcccact
    47161 gatcaactga tcaaaatacg gatgattggt ataaggcaga atcactccaa tgcttttcgt
    47221 ttttccataa cttaactgtc tggcatttgc attagggata taatcgactt ctttcatgac
    47281 cgcaagtatt tctttccttt tttcctcatc tacatatgga tgtccattga ttacacgtga
    47341 aatagtagaa actgaataac ctgtgagctt tgcgatatct cgaatgtttg ccactgctgc
    47401 ttcgccacct atacaaaaag attttcttgt aaatagtcta ccacagctgg accgcattca
    47461 aaaaactgat acatttttcc ttcatgcgaa aaactgatcc gctgcccgcc ccaatatata
    47521 tcttttgtca aattttcgat ttcattcatt ggtatttctt ttactatatt cctattcaac
    47581 ccttgaaaac tgtacaacgt taatacggat cccttgaact cgacaaacgc ataaaaataa
    47641 ttgttcgttg tgccatctgt tatctctaca taagtattca ttgcaagccc tccttttgat
    47701 actttgagta tagtgtatga aacgcgtctc atagcaagga gtttgttttg ccaaagaaaa
    47761 gttttattcc tctttcatta ttaccaaagt ctcttatttt aaattgaata cattataatg
    47821 atactgtaaa tatatcttta ttattagtga atggatattt acttttaata gataaaatat
    47881 tataactatt aaaaataact attttcatta taattacaca caaaaaagag cgtgagacaa
    47941 aagtaaagaa c
//
LOCUS       DACDKM010000011        45918 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20834_2016
            SAMN12640370-rid9891663.denovo.011, whole genome shotgun sequence.
ACCESSION   DACDKM010000011 DACDKM010000000
VERSION     DACDKM010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640370
            Sequence Read Archive: SRR10040891
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 45918)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 45918)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742835.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742835.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:04:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,012
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 29 of 75
            Pseudo Genes (incomplete)         :: 33 of 75
            Pseudo Genes (internal stop)      :: 19 of 75
            Pseudo Genes (multiple problems)  :: 5 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..45918
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640370-rid9891663.denovo.011"
                     /isolate="E20834_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(224..457)
                     /locus_tag="IV334_000625"
     CDS             complement(224..457)
                     /locus_tag="IV334_000625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002382893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-dependent repressor"
                     /protein_id="HAP8942719.1"
                     /translation="MVQIKTTTILIYLLAIQELSKRKKKLKNNDLAKVLSVSPASVSE
                     MLAKLRYEDYLDIGFQLTDKGKKFIDDYKKGFK"
     gene            complement(472..1422)
                     /locus_tag="IV334_000626"
     CDS             complement(472..1422)
                     /locus_tag="IV334_000626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HAP8942720.1"
                     /translation="MMRKWKAVLGSLGILIALFIFGACSINSKDKDKVASNEKLKVVV
                     TNSILADITENIAKDKIDLHSIVPIGKDPHEYEPLPEDVQKTSKADLIFYNGVNLETG
                     GNAWFTKLVKNANKEENKDYFAASDGIDVIYLEGQSEKGKEDPHAWLNLENGIIYAKN
                     IEKQLAEKDPDNKKFYKENLDKYIEKLDSLDKEAKSKFASIPNDKKMIVTSEGCFKYF
                     SKAYNVPSAYIWEINTEEEGTPDQIKHLVEKLRTTKVPSLFVESSVDDRPMKTVSKDT
                     NIPIYSTIFTDSIAEKGQDGDSYYAMMKWNLDKIAEGLSK"
     gene            complement(1419..2261)
                     /locus_tag="IV334_000627"
     CDS             complement(1419..2261)
                     /locus_tag="IV334_000627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HAP8942721.1"
                     /translation="MLQNFINGLYDFHFLQNALITSVVIGIVSGTVGCFIILRGMSLM
                     GDAISHAVLPGVAISYILGINFFIGAIVFGLLASIIITFIKSNSVIKGDTAIGITFSS
                     FLALGVILIGVANSSTDLFHILFGNILAVQDIDKWITIGVSVLVLVIVVLFFKELLIT
                     SFDPLMAKAIGMNVSFYHYLLMVLLTLVAVTAMQSVGTILIVAMLITPAATAYLYANS
                     LKTMIFISATVGASSSLLGLFIGYSFNIAAGSSIVLTAAFLFVISFLISPKQKFLRKK
                     ERSL"
     gene            complement(2263..2982)
                     /locus_tag="IV334_000628"
     CDS             complement(2263..2982)
                     /locus_tag="IV334_000628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HAP8942722.1"
                     /translation="MLEVKKLNVCYNDFLALNEISLKIQEGALTGIIGPNGAGKSTLL
                     KAMLNIIPHQGEVSINNEDINKKLVKIAYVEQKADIDFTFPIKVKECVSMGTYAGMKV
                     FQRIKNAEWQRVSKALEKVDMGKYSNHQIGELSGGQFQRVLLARCLAQNADFIFLDEP
                     FVGIDLVSERIIMDTLKKLKHQGKTVLIVHHDLSKVKKYFDNIILLNRNLIAHGSVES
                     VFNEENLKKAYGDTIFIGEGE"
     gene            3674..3832
                     /locus_tag="IV334_000629"
     CDS             3674..3832
                     /locus_tag="IV334_000629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP8942723.1"
                     /translation="MNKKQTCAFLGIDNNTLDKWIRIYNFPVIRVGGVVRFDVEDIKD
                     FMNTFKIT"
     gene            3904..5130
                     /locus_tag="IV334_000630"
     CDS             3904..5130
                     /locus_tag="IV334_000630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAP8942724.1"
                     /translation="MPTKLSNGKYKTNLRYPKRFKEITGIASEKYQKTFPNRQLAIKA
                     ENDMKKKIEKVLREENANSLELKGKITFKKFYESKWLPRYELGQTIRSNRPPSDITIS
                     NTKDIFRLHILPMFGEYAMNYLNINTEIISDELTKKSKEYANIKIIKGYVRSMFDIAE
                     ILNYIEFNRTTKIIQSITAPKKNALEEKRIQEGKQALSSKELTNWIEAVNDDFNNHLL
                     TFHDYTLFMITLYLGDRKSETYALQWKYIDFEKQTVRLKHTLDKYQRKKFTKGRKDTV
                     IQVPEVVMTLLSEWKSVQADQLLKLKIKQTLDQYLFTYTKPSGEVNCPVHADYLNYRI
                     NSIKRRHPDLVHLSPHKLRHTYATIARQGGADMNQISNALTHSDISTTKIYVNTPDIV
                     DKAVFEAFQRGLNKCD"
     gene            complement(5218..5610)
                     /gene="rpsI"
                     /locus_tag="IV334_000631"
     CDS             complement(5218..5610)
                     /gene="rpsI"
                     /locus_tag="IV334_000631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002942223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HAP8942725.1"
                     /translation="MAQVQYIGTGRRKNAVARVRLVPGTGKITVNKKDVEEYIPHADL
                     REVINQPFAVTETKGAYDVFVNVNGGGYAGQSGAIRHGIARALLEVDPDFRLALKRAG
                     LLTRDARMVERKKPGLKKARKASQFSKR"
     gene            complement(5626..6069)
                     /gene="rplM"
                     /locus_tag="IV334_000632"
     CDS             complement(5626..6069)
                     /gene="rplM"
                     /locus_tag="IV334_000632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HAP8942726.1"
                     /translation="MRTTYMAKPGEVERKWYVVDATDVPLGRLSTVVASVLRGKNKPT
                     FTPHLDTGDFVIVINADKVKLTGKKATDKIYYRHSNYPGGLKSITAGELRAKNSRRLI
                     ETSVKGMLPKNTLGRKQFTKLNVYGGAEHPHAAQQPEVLDITNLI"
     gene            complement(6588..7055)
                     /locus_tag="IV334_000633"
     CDS             complement(6588..7055)
                     /locus_tag="IV334_000633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein"
                     /protein_id="HAP8942727.1"
                     /translation="MKFQQTKEVLNQLVADLSQMSVVVHQTHWYMRGPGFLTLHPMMD
                     EFMDDLNAQLDEVSERLITLDGSPYSTLREFADNTKIPDEIGRWDRTIPERLETLVAG
                     YRYLADLYQKGIEVSGEEGDDPTQDIFIEFKTATEKRIWMIQAHLGKAPEIDA"
     gene            complement(7175..8713)
                     /locus_tag="IV334_000634"
     CDS             complement(7175..8713)
                     /locus_tag="IV334_000634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HAP8942728.1"
                     /translation="MNVSIGLDIGTTQTKAVAFTDEAQEAASAYFRYPLIQETQGMAE
                     QDQELIFQGVCTVIKEVIKQLPSDTIIQFVSFSSAMHGLILLDEEKTPLSRMITWADN
                     RAADEAEKLKYTVLGKEFYRQTGTPIHPMTPLNKIKWLQNSQPELFKKTAYFVGIKDY
                     IFYRLFGELISDYGTASGTGYFDIHEFQWAEEVLDYLNIRIEQLPQVFPSTYQVTGLP
                     SKTAAELGLPNDISFVLGGADGPLSNLGLGAFGDTIAALTVGTSGALRFITTQPQFHP
                     EMETFCYVLDQKHWVVGGATSNGAGIFDWAGHTFLQEVVQSAKEKGENPYDALLSAIA
                     FVPAGANGLIFHPYLLGERAPLWDADAFGSFIGLQRQHTEKEMMKAILEGICLNLYRI
                     LKGVCQENQLTEIRATGGFAQSPIFRQMMADVLGYPLVFPTVTEASALGAVILGWRSI
                     GKIQSISEAQELIELKEQVGVQETDHKKYQKIYPLFVSTQKHLAQTYQQFAQLREELA
                     DSEK"
     gene            8793..9479
                     /locus_tag="IV334_000635"
     CDS             8793..9479
                     /locus_tag="IV334_000635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HAP8942729.1"
                     /translation="MLLYFIIGCCIGSFLCLTAQRIPLGHSIIYPRSHCVKCCRSLSW
                     YELIPILSIIVQRFRCRYCRCRLPFYYLLAEALCGGLFAWFFTFSSARNFSTFIWMLS
                     ALLFSLMDLFYFMVHSHTLFCSWAILWIFWLQAGVFQWQSVLLTFIVGGVCLRYGQSY
                     LGAGDTLLMLSWSGGLSLEELLQILFLASLFGISVFSVYFICHKKKLEKLPFIPFLSS
                     ALLIVLHLRY"
     gene            complement(9680..10207)
                     /gene="msrA"
                     /locus_tag="IV334_000636"
     CDS             complement(9680..10207)
                     /gene="msrA"
                     /locus_tag="IV334_000636"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HAP8942730.1"
                     /translation="MTKKAVFAGGCFWCMVKPFEEQPGIISVTSGYTGGHVPNPTYEQ
                     VTTGTTGHTEAVEIEYDPEVISYDQLVEIYWQQTDPTDALGQFADRGDSYRPVIYYND
                     EEERNIAERSKQALQDSGRFDKPIVTKIEPRSEFYPAEDYHQDYYKKNKLHYNLYREG
                     SGRAGFLRRHWGVKK"
     gene            complement(10291..10968)
                     /gene="pgmB"
                     /locus_tag="IV334_000637"
     CDS             complement(10291..10968)
                     /gene="pgmB"
                     /locus_tag="IV334_000637"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HAP8942731.1"
                     /translation="MKIKAALFDLDGVLVDTARYHYEAWLVLANQLSIPFTEKENEQL
                     KGISRTESLERLLSFGKMEQKFSEKEKSAFAEQKNNLYLQAIQKMDETSVLPGAIAVL
                     EYLKKTNIKIGLGSASKNARLILEKTNLTSYFDVLIDGTQVSKAKPDPEVFLKGAQQL
                     NVPPNACLVIEDSEAGCQAALAGNMHVLGIGENINLPSAEYVIPDLTVFDQVRSFWHL
                     SEAVSRI"
     gene            complement(10965..12095)
                     /locus_tag="IV334_000638"
     CDS             complement(10965..12095)
                     /locus_tag="IV334_000638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HAP8942732.1"
                     /translation="MRILIAPDSFKESLTALEAAQAIQSGFQEVFVQAEYDLLPIGDG
                     GEGTVSSLADSLRLKRQTAVVTNAFGKNKEIQYASDGQTAIFEMAEIVGLKDVPLNQQ
                     QPLRLSTRGVGELIRQLLQQGVRHIIIGVGGSSTNDGGIGMAEGLGYAFYDQDNQKVE
                     ALGENLGKIATVDKTNVPIELIKAKITVITDVTNLLCGKEGASFVFGPQKGLKENELA
                     VADQAMHDFYMRFAPQVIEASGSSAGGGMGAGLLAFASGKIVSGIDYVLDLLEFDKRV
                     QQADLVIVGEGRLDGQSLKGKAPVGIARRVPEGVPVIAICGSVGKGSEKAADVGISAV
                     FPILPAAVPLQEALQQATDNLHRTAKNIASLLQVVHFQGGNR"
     gene            complement(12095..14278)
                     /locus_tag="IV334_000639"
     CDS             complement(12095..14278)
                     /locus_tag="IV334_000639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 65 protein"
                     /protein_id="HAP8942733.1"
                     /translation="MKVEEKQFDLEYINKYASQMTIGNGFFGIRGSQEEDYRNQVRGM
                     FAAGVYNRPLGAESAELVNLPDVIRWEIKIDGEHFSLETMKVLAYERALDLEMGELKR
                     YALIETAQGKKVEVITCRVADQNNLHQAALKVVIKPLTEDCQVDVKTGIDGQQTNFGT
                     QQLVEKELRVFGDDLISASYQTTESKIDIGIAAKFNKAGVFQAKNRRITASFSEKIRK
                     NTSFTLEKIIVLSTSLQQTDPFKKSIAQAQKSVSYTEILEQSMSYWTDFWHQHRITIQ
                     GDSSFDQLAMDFAAYHLQIMTPRNDPSVSVGAKGLTGEGYKGHVFWDTEIFILPFFLY
                     QFPEVAKNLLLYRYDRLEGARQKARDYHYQGALFPWESAHTGQEETPKFAALNIKTGT
                     RQEVASAAAEHHISADLAYAVQEYVQATEDQSFLETKGKTLIHETAAFWLSRAVKVDD
                     HLEIHRVIGPDEYTEYIDNNAYTNYLAHYNVALAVKYQAGDPLFLKECQNFLDKLYLP
                     VPNKEQLIPQDDTYLSKPVIDLTTYKERPGSQSILLDYARSEVNELQVSKQADLVMLL
                     YLLPEYFSQPIVSKNLHYYENKTIHDSSLSKAIHAIEACRIQEMEWAYHLFQEACLID
                     LGPDPHSSDDGIHAASLGALWLAVIFGFSGITVTNGTLSISPRLPSAWEKISFPFIWK
                     NHRLLFVLTKKQIVIKKETKETLPIFVNGDQYQLEKELILERRGK"
     gene            complement(14275..15300)
                     /locus_tag="IV334_000640"
     CDS             complement(14275..15300)
                     /locus_tag="IV334_000640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HAP8942734.1"
                     /translation="MKKVAIIGAGQVAEKVHAAYYQKRSDVLELAAVVDPVSQRAEDF
                     CRRTGFSHAYTSVTQMLNEISPDIVSVCTPNRFHFEHVLAALEANASVMCEKPPAMTA
                     DQARKMAETADKNGQILAYGFQHRFSDEATMIKNHLDVLGDIYYVEANALRRSGVPGW
                     GTFIDKELQGGGPLIDYGIHMLDTAMYITGFPKVKSVRAYSFQKIGTKKNSGKFGKWD
                     PSKFTVEDAIFGTLELENGKIIQLNTSFALNIREDKLNVAFCGDQAGANLYPGELFED
                     VDGKLVSLQKTTPVNDPHELSMAHFVNRALGDQTQMIADGWQGYVIQNLVENLYRSAE
                     TGEVITL"
     gene            complement(15345..16172)
                     /locus_tag="IV334_000641"
     CDS             complement(15345..16172)
                     /locus_tag="IV334_000641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HAP8942735.1"
                     /translation="MKFGTQDQAFFPRDILEKFRFIKDCGFEEYEIDGKLLIENIDTV
                     KQAIKETGIPVVTACNGYDGWIGDFIEERRLNGVRQIAEILRALSEVGGKGIVIPAAW
                     GMFTYRLPPMVSPRSKEGDYKAVSESLRYLDKVAEETGTVIYLEPLNRYQDHMINLLA
                     DARHYIDSNQLKHVQIIADFYHMNIEEDDISKALTTQKEYVGHIHLADNHRYQPGSGA
                     IDFTRHFQTLLQNEYEGYMVYECRLRGENEVKIYQESLQYLKNCLETARHLNDLEKE"
     gene            complement(16169..17236)
                     /locus_tag="IV334_000642"
     CDS             complement(16169..17236)
                     /locus_tag="IV334_000642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase"
                     /protein_id="HAP8942736.1"
                     /translation="MKKLVALEPRIAGFKDYEDREIEKNEVRIRVIFATPKPGTEIVD
                     FRGLSPFIDEEFSEEWRLFLPREEGSQRGVVFGEFQLGNMIVGTIEETGSLVTEYQKG
                     ELVCTYGPIRETLITNGVDNYKLRKLDRLDQWKNAVCYDPAQFALGGIREAHVRAGDA
                     VAIFGLGAIGQIAIQLAKKAGATTIVGIDPIAQRRELAEKHGADYTLDPMKADVGFRL
                     KELTDKRGVDSVIETSGSAAALQASLIGIGYGGIISYVAFAKPFPAGFNLGREAHFNN
                     AKIVFARASSEPNPDYPRWNRKRIEDTCWELLMNGYLDCEEIIDPVVPFAESAEAYMR
                     YVDQHPEESIKMGVQVRGGEQ"
     gene            complement(17262..18098)
                     /locus_tag="IV334_000643"
     CDS             complement(17262..18098)
                     /locus_tag="IV334_000643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAP8942737.1"
                     /translation="MKNKKSRKILFYATILLLLAVNLFPFLIMLMTSFKDSQEAIATT
                     QTIFPKEWTFEHYQDIFNAEIFPFIMYFKNSLVISLIASVTAVVIGISGAYALSKLRF
                     KARSKINASFYTVYMFSGILIVVPLFKIISGLGLYDTNTALIITMVVQTLPTAIFMLK
                     SYFDTIPTDIEEAAMIDGLSRIQIIIRIIIPLAISGIISVFVYCFMVSWNDYLFASIF
                     LSTSEKFTLPIGLNTLFSTPDYIWGRMMAASLVTALPVIIMYALSERFIKGDLVEGGV
                     KG"
     gene            complement(18095..18964)
                     /locus_tag="IV334_000644"
     CDS             complement(18095..18964)
                     /locus_tag="IV334_000644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP8942738.1"
                     /translation="MNRPRKHSDLKLAMGLLAPSMLLLAVLVLIPMLSNIQISFFDMP
                     LNPNSDAVFIGLSNYLEILSDSEFYRSLGLTVAYTFLVVAGSTALGLAVAILLNRKFR
                     FRKTVRSLILLSYVTPSISLIFAWKYMFNNSYGIVNDLLVDRLQLFNEAPLWFDQPVA
                     SFFMVVLFAIWRYYPYAFISFLAILQTVDKTLYEAAEMDGANAWQKFKIVTLPAIKPV
                     LATVIVLRSIWMFYMFTDVYLLTNKVNILGVYLYQTAFAFNDLGKAAAISILLFIIIA
                     AVVLLSRKKVDLK"
     gene            complement(19034..20317)
                     /locus_tag="IV334_000645"
     CDS             complement(19034..20317)
                     /locus_tag="IV334_000645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HAP8942739.1"
                     /translation="MKKIIVTTCLLATGFLLTACGESQSSNESQQTTIQFMHSSVEQE
                     RLSVINELVADFEKENPDIKIKQVPVEESAFNTKIVTLARSGKLPEVMEVSQDFAKVM
                     DKDELIDQTAVQNVIEEAGEDNYYDGAKNLVRSEDGKSYIAAPISGWVQGIWYDKEKL
                     SEAGFSEPENWDDILKIAQHFTDKENKQYGIAMPTAEGTMSEQAFSQFALSNGANVLD
                     DEGEVTIDTEKMREALSFYQNLSQYTMPGSNDVTEIKDAFMNGTAPMAIYSTYILPSV
                     YEAGNSENIGFAIPTQKDQAVYGTVSGLTISSGLDEKQKEAAEAFTAFLSQPKNMEKW
                     VLMSPGGAQPVNKQVVELDGYKENEVIKSFGELPSEIASAFDEVQVFGLVGEKNFTKM
                     GDITSSGVIGKAVNQVTVGNEDVDQALADAQKNTK"
     gene            complement(20331..22016)
                     /locus_tag="IV334_000646"
     CDS             complement(20331..22016)
                     /locus_tag="IV334_000646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphorylase"
                     /protein_id="HAP8942740.1"
                     /translation="MYTKLLQAIYRDSDLSEKTASMIEQKVSVYQQKKGQAGKLHWDE
                     EDIFLITYGDQFFEEQQPTLRTFKKFYQKYLADTFRVVHFLPFFPYTSDDGFSVVNYQ
                     AINDRLGTWEDIEEMRSETRLMFDFVCNHMSAESTWFQEYLNLNDTYKDFFIDLPLDT
                     DTSSVTRPRTSPLLTTFIDKNGKEKHVWTTFSEDQVDLNFKNPEVLLKMIDILLFYLE
                     KGASFIRLDAVGFLWKELGTSCIHLPETHKIIQLFRAIADEAAPGTVLITETNVPHKD
                     NISYFGNGENEAQMVYQFSLPPLTLQAIRSGNTSKLKEWLKTIDFASKKTTFFNFLAS
                     HDGIGLNPVRGILPEKTILELVEQLKKEGARVNYKKNPDGTTSPYEINATYLDALSSP
                     TDTTEWRSKRFLAAHSFLVTLPGVPAIYIQSVLGSRNDYKGVEKTGENRKINRQKFLL
                     ADIEKELQTDPLRTAVYGELTQMLSIRKKEKAFHPNSLIYLLDSADSFISFIREAKEE
                     KVLVIHNFSNQSAGYLLPKGDYLDLFSGQIIHCSQERKLIFQPYEYMWLKIIK"
     gene            22291..23316
                     /locus_tag="IV334_000647"
     CDS             22291..23316
                     /locus_tag="IV334_000647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAP8942741.1"
                     /translation="MKPTIYDIAKKANVSKSTVSRVLNNQPNISDKAKASVLQAIKEL
                     NYEPSKIARGLSGNGFDAILALSHRTSHTTYGNPFFSDVIQSISAVSEQNNFDLILQT
                     AKTPEEELEKCAKKIQEKMIRGIIILSSTMDETLLFELDQYNIPIVVIGKVGNDYTNI
                     YSVDTDNFQDSYDLVETLIKNGHRHIGCLHAPTEVYVSNDRLNGYRQCLFDHHLDIRN
                     EWIIDGGFRLEDSMAAMNDLMKLRERPTAIFSTDALKSLSIYKVAEQSGKQIPDDLSI
                     AGFSDPLLSSLFSPQLTRIDIPTNQLGIKATELLFQLIHHQYPEEKRIILSTEITLTD
                     SIQQQEM"
     gene            complement(23911..27564)
                     /gene="rpoC"
                     /locus_tag="IV334_000648"
     CDS             complement(23911..27564)
                     /gene="rpoC"
                     /locus_tag="IV334_000648"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HAP8942742.1"
                     /translation="MIDVNKFESMQIGLASPEKIRSWSYGEVKKPETINYRTLKPERE
                     GLFCERIFGPTKDWECACGKYKRIRYKGIVCDRCGVEVTRSKVRRERMGHIELAAPVS
                     HIWYFKGIPSRMGLVLDMSPRALEEIIYFASYVVIEPGNTNLEKKQLLTEREYREKRE
                     QYGQEFQAAMGAEAIKQLLDNVDLDAEAAELKEELKSAQGQKRTRAIRRLDILEAFRA
                     SGNQPSWMVMDVIPVIPPDLRPMVQLEGGRFATSDLNDLYRRVINRNNRLKRLLDLNA
                     PGIIVQNEKRMLQEAVDALIDNGRRGRPVTGPGNRPLKSLSHMLKGKQGRFRQNLLGK
                     RVDYSGRSVIVVGPFLKMYQCGLPKEMAIELFKPFVMRELVQRELASNIKNAKRKIER
                     QEDEVWDVLEDVIKEHPVLLNRAPTLHRLGIQAFEPVLVQGRAIRLHPLVCEAYNADF
                     DGDQMAVHVPLNEEAQAEARMLMLAAQNILNPKDGKPVVTPSQDMVLGNYYLTMEEEG
                     REGEGMVFRDQNEAVIAWRNGYVHLHSRIGVNPNSLGEKPFTEWQKERTMITTVGKII
                     FNEIMPPEFPYLNEPTDFNLTVQTPDKYFVEAGTDIPAHIKEQELVLPFKKKNLGNII
                     AEVFKRFKVTETSKMLDRMKDLGYKHSTHAGITVGIADISVLNEKQEIIENAHKQVET
                     ITKQFRRGLITDDERYERVIAVWNAAKDSIQQKLMEGLEAKNPIFMMSDSGARGNISN
                     FTQLAGMRGLMAAPNGRIMELPIISNFREGLSVLEMFISTHGARKGMTDTALKTADSG
                     YLTRRLVDVAQDVIIREEDCGTDRGLEIQAIREGNEIIESLEDRLVGRYTRKEVRHPE
                     TNELIIAGNQLISEDIAKQIVDAGVETVTIRSVFTCNTKHGVCKHCYGRNLATGSDVE
                     VGEAVGTIAAQSIGEPGTQLTMRTFHTGGVAGDDITQGLPRVQEIFEARNPKGQAVIT
                     EVTGDVIDISEDPATRTKEVTIKGKTDTRTYTVPYTARMKVAEGDMIHRGAPLTEGSI
                     DPKQLLQVRDVLSVENYLLREVQRVYRMQGVEIGDKHIEVMVRQMLRKVRVMDPGDTD
                     ILPGTLLDIADFKDQNYNTLVSGGVPATSRPVLLGITKASLETNSFLSAASFQETTRV
                     LTDAAIRGKKDPLLGLKENVIIGKIIPAGTGMQRYRDMEPKEVGVASENVYSISDIEA
                     QMAAEDAMNNQTK"
     gene            complement(27643..31260)
                     /gene="rpoB"
                     /locus_tag="IV334_000649"
     CDS             complement(27643..31260)
                     /gene="rpoB"
                     /locus_tag="IV334_000649"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HAP8942743.1"
                     /translation="MAGHVVKYGKHRERRSFARISEVLELPNLIEIQTDSYQWFLDEG
                     LREMFEDILPIDDFNGNLSLEFVDYELKEPKYTVAEARAHDANYSAPLHVTLRLTNRE
                     TGEIKAQEVFFGDFPLMTEQGTFIINGAERVIVSQLVRSPGVYFHGKVDKNGKEGFGS
                     TVIPNRGAWLEMETDAKDISYVRIDRTRKIPLTVLVRALGFGSDDTIFEIFGDSETLR
                     NTVEKDLHKNASDSRTEEGLKDVYERLRPGEPKTADSSRNLLNARFFDPKRYDLANVG
                     RYKVNKKLDLKTRLLNLTLAETLVDPETGEIIVEKGTVLTHQVMETLAPFIDNGLNSV
                     TYYPSEDGVVTDPMTVQVIKVFSPRDPEREVNVIGNGYPEAAVKTVRPADIIASMSYF
                     LNLMEGIGNVDDIDHLGNRRIRSVGELLQNQFRIGLARMERVVRERMSIQDTETLTPQ
                     QLINIRPVVASIKEFFGSSQLSQFMDQTNPLGELTHKRRLSALGPGGLTRDRAGYEVR
                     DVHYSHYGRMCPIETPEGPNIGLINSLSSYAKVNKFGFIETPYRRVDRETGRVTDQID
                     YLTADIEDHYIVAQANSPLNEDGTFAQDVVMARAQSENLEVSIDKVDYMDVSPKQVVA
                     VATACIPFLENDDSNRALMGANMQRQAVPLINPQAPWVGTGMEYKSAHDSGAALLCKH
                     DGVVEYVDASEIRVRRDNGALDKYDVTKFRRSNSGTSYNQRPIVHLGEKVEKGDTLAD
                     GPSMEQGEMALGQNVLVGFMTWEGYNYEDAIIMSRRLVKDDVYTSIHIEEYESEARDT
                     KLGPEEITREIPNVGEDALKDLDEMGIIRIGAEVQDGDLLVGKVTPKGVTELSAEERL
                     LHAIFGEKAREVRDTSLRVPHGGGGIVHDVKIFTREAGDELSPGVNMLVRVYIVQKRK
                     IHEGDKMAGRHGNKGVVSRIMPEEDMPFLPDGTPIDIMLNPLGVPSRMNIGQVLELHL
                     GMAARQLGIHVATPVFDGASDEDVWETVREAGMASDAKTVLYDGRTGEPFDGRVSVGV
                     MYMIKLAHMVDDKLHARSIGPYSLVTQQPLGGKAQFGGQRFGEMEVWALEAYGAAYTL
                     QEILTYKSDDVVGRVKTYEAIVKGEPIPKPGVPESFRVLVKELQSLGLDMRVLDIEET
                     EIELRDMDDEDDDLITVDALTKFAEQQTAKELEKKAAEQVEDEKDDVIQNFETAEDNL
                     D"
     gene            31988..32956
                     /locus_tag="IV334_000650"
     CDS             31988..32956
                     /locus_tag="IV334_000650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAP8942744.1"
                     /translation="MKKNIIIGLGAALLLALGGCAQKDNSAEASDGQKTIGVLQLVQH
                     NSLDAAYKGFKEGLAEEGYKEGDNLTIDYQNAQNNQDNLKSMSEKLVKEDPDLLLGIA
                     TPAAQSLLNETTTIPMTVTAVTDLEQAHLVTSNEKPGGNVTGTTDMVPIEKQIGLLLN
                     IVPDAKTIGIMYNNGEANSKIQADLAEKELKKAGVKVKVLTANSTNDVQQVTKSLAKD
                     VDGIYIPTDNTFASAASVVGEVAKETKTPVVAGAVDQVKDGGLATVGIDYESLGKQTG
                     IMAAKILDGKNPSELPVETANDLELYVNKDMAKALGINPDSIKEAE"
     gene            32974..33879
                     /locus_tag="IV334_000651"
     CDS             32974..33879
                     /locus_tag="IV334_000651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP8942745.1"
                     /translation="MTNLLSLFLSSASQGVLWALLAIGVYVTFRILDIADLTAEGSFP
                     LGAGIAAVSITNGYHPLTACLLGFLGGAAAGLVSGLLHTKLKIPALLAGIITMTGLYS
                     VTSRVMGAPNISLLGQPTIFHWVESFGITKDNAVLIVGLLFALIVVCLLVLFLRTETG
                     LAIRATGDNLAMSEANGINTDNMKIIGYMISNGCIALSGSLLAQNNGFADLNSGIGTI
                     VIGLASIIIAEVLLRNQPLFLRLLTVVLGAVIYRFILALVFELNVEPSDSKLASAFVL
                     VICLSFPQIQQKFHLPQKKKGGSVK"
     gene            33876..34682
                     /locus_tag="IV334_000652"
     CDS             33876..34682
                     /locus_tag="IV334_000652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAP8942746.1"
                     /translation="MNDVLTLENLHHTFEKGTINENHVLKGINLSLDPGDFVTIIGGN
                     GAGKSTLLNSIAGTFSVDQGQILLNGKNITKKSVVERSKSISRVFQDPKLGTAVRLTV
                     EENLALAMKRGKKRGFFRGVKPQDRTFFKDQLVRLNLGLENRLSSEIGLLSGGQRQAI
                     TLLMATLQRPELILLDEHTAALDPATSKTVMELTEKLITEQNLTAFMVTHDMEDAIRY
                     GSRLIMLHQGRIVVDIQQEEKQQLTVPQLLALFKQNSGTALKDDQVLLSS"
     gene            34839..35807
                     /locus_tag="IV334_000653"
     CDS             34839..35807
                     /locus_tag="IV334_000653"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HAP8942747.1"
                     /translation="MTTKDKVLQLLKKTPDFLSGEKLAQQLEVSRTSIWKAIKELEKA
                     GYRFEHGPTGYRYLPSDVLDAKEIQQELRSFIPDLKVQTMLTSESTMKDAKLAAAQGD
                     SVSKLFVADTQEQARGRFGRPFFAEPGRGIYMSLLLHPDKRFDELPQYTIIAASACVK
                     AIESVTGRSASIKWVNDIYMDGKKVCGILSEAVSDMESGRISHIIIGIGLNFSVPQNH
                     FPADLQEKAISVFPDGQASVTRNQLISKIWQELFTYLSHPEKALDFYKEKSFVLGKTV
                     SFNQKGTTYVGLAKEIGDHGELIVQTNEQKMSLSSGEISLTSIRTQ"
     gene            complement(36124..36648)
                     /locus_tag="IV334_000654"
     CDS             complement(36124..36648)
                     /locus_tag="IV334_000654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HAP8942748.1"
                     /translation="MDYLDMLCESVDRAYDRFERLFEGVSIEEANRFPVEDRAPQIKS
                     MTWLAWHTARELDFQIADLAGTSPIWHSQDWESRFPMTIADNEQDWKHSLEQAKQIQA
                     NQIEDLLGYLRGAADFAISYLRRLEPSELDEVIDENWTPPVTRGMRLVSIIDDAAMHS
                     GQVIYARRLLGLRD"
     gene            36935..38746
                     /gene="scm"
                     /locus_tag="IV334_000655"
     CDS             36935..38746
                     /gene="scm"
                     /locus_tag="IV334_000655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Scm"
                     /protein_id="HAP8942749.1"
                     /translation="MKKRASLLVLVALLFNIFSYGVAVSAETYIPKDPGNIYNDLTGN
                     YGLLGIASQFHVFAKNKTTVNAHTDGNIATHELDAKNNFGTELHEGLLNQEINYIQKT
                     DSFANSSGIPTGSTRMNKFVVGSNVDVALQDGHPTVNGSRLDHLTPDDFYKDRAGHEY
                     IDFEQEFAKLNVAANDLATITPAKTYTAADFPDMNNRTIDLTDLSDTGFLLVNIDAEV
                     LTMNTPLQIINPNDQVVVFNVINSSSALNVQSPIKYNDRSNHETEDFSDANISWNFGN
                     EMTDLTISAPFQGTILAPNATIRITQNQDGTIIGTNVILDAATNRWDPNEIFISDNGT
                     DTTDTSDSTDTSTTSDSSDSSTSSDSTDTTSTTSDSTDTSASSDSTDTTSTTSDSTDT
                     SASSDSTDTTSTTSDSTDTSASSDSTDTTSTTSDSTDTSASSDSTDTTSTTSDSTDTS
                     ASSDSTDTSTTTSDSTDTSASSDSTDTTSTTSDSSDSSTTTSDSTDTSASSDSTDTSS
                     TTSDSSDSSTSSDSTDTSSTTSDTRHASVSFSKTSSDNRTNFNLTNKTSNDNGNKSKR
                     ALPKTGSQSNNWITLAGVILLVIGLRITFMSYKKSKR"
     gene            complement(38901..39887)
                     /locus_tag="IV334_000656"
     CDS             complement(38901..39887)
                     /locus_tag="IV334_000656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HAP8942750.1"
                     /translation="MKLHPMWENYPALSKELTETLKRMEETVRLKNKPVEQAIKETIH
                     AGGKLLRPAYQLLFSQFGPEQDRDKAIALAASIEMLHTATLIHDDIVDEADLRRGQPN
                     IRSQFGNVVSVYAGDYLFVCCFKLLSDYSTSLKSLQLNSRSMEKVLNGELGQMDDRYN
                     YELSVKEYLENISGKTAELFQLSCSVGAFESGTSERFAKKAGDIGLSIGMAFQIIDDI
                     LDYTKESQEIGKPVLEDMRQGVYSLPLIYSLQKNKPKLLPYLEKKAAMTEEDVDAVRK
                     IVEHTGGVEEARKLAASYTEKALKEIKKLPATSLRTKENLFSLTQLILDRKD"
     gene            complement(39910..40476)
                     /locus_tag="IV334_000657"
     CDS             complement(39910..40476)
                     /locus_tag="IV334_000657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gx transporter family protein"
                     /protein_id="HAP8942751.1"
                     /translation="MSKLQKMIYISLLVAQGVIIGLLENMIPYPFSFAPGAKLGLANL
                     ITIVAIFTMKKQDSFLLLWLRLFLTTLLGGTISTFLYSMSGALLSYFGMLLVKQLGPK
                     RVSIIGISAAGGFMHNVGQLLMASFIAQSWTVMLYLPVLSVLGILSGIAIGIAANYLL
                     RHVQTLQRFQKDYEQGTKQKWSPMFTKK"
     gene            complement(40490..40906)
                     /locus_tag="IV334_000658"
     CDS             complement(40490..40906)
                     /locus_tag="IV334_000658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NusG domain II-containing protein"
                     /protein_id="HAP8942752.1"
                     /translation="MSLKEFAEKSLLKPWDVFIILLLTVLSFLPVMIFGLQQNTATAE
                     KEAVLRVDGTEIKTFPLMAGEESNTYTYKDEHGDYNLIEIDGDRVRIKEADCDDQICV
                     RRGWATKNGETIVCLPHKLVIEVRSTDGGDEDSLIY"
     gene            complement(41363..43297)
                     /locus_tag="IV334_000659"
     CDS             complement(41363..43297)
                     /locus_tag="IV334_000659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016186137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HAP8942753.1"
                     /translation="MAKQNIVVVGAGYAGVSATKYLAKKLKKEDVAITLIDRHSYHTM
                     MTELHEVAGGRVEPEAIQYDLQHLFARQKNVQLVTDTVIGIDKERKIVKTKLGSYPFD
                     QLILGMGGEPNDFGTPGVKAHGFTLWSFEDSVRIREHIEKTVAKAALEPDDELRKAML
                     TFVVCGSGFTGIEMIGELIDWKNRLAKDYKLDPNEITLMVVEAMPTILNMLDRNDAAK
                     AERYLRKHNVELLLNAPIVEVAADHIKLKDGRNIPTHTLIWTAGVKGTSDAADFGLES
                     ARGQRLIANEYMQAKGYEDKNIYIIGDLVFYEEFPGTPTPQIVQAAEQTGHTAAANIV
                     ASIKGGEKHKFKGNYQGFMVSIGAKWGVANLFDKIHLSGFLAIVMKHIVNLKYFFDIR
                     SGYYMFQYLMHEFFHIKDDRNVTRGHSSRYGNVLWSVPLRIFYGLVWFVEASKKIIGN
                     GEVFKPSTWFGEGSWFTGNSVFPWTWEYNVTDVASGASAAGDATTAASGAGASAGEAA
                     TQAAHFGLSYAYGEEPMAVIEKSPDWFTKIMQVIMPNNDVAMFFQKFMVFFEIALALA
                     LIAGLFTWLCSATTIVLVITFCLSGMFYWVNIWFIFVAFALMNGSGRAIGLDRWVIPW
                     LQRTLGNWWYGRPSSQYGKK"
     gene            43599..44534
                     /locus_tag="IV334_000660"
     CDS             43599..44534
                     /locus_tag="IV334_000660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein"
                     /protein_id="HAP8942754.1"
                     /translation="MKKKRFVTGFAVLAFSALVLGACGADDNGSSNSSSESSTAQSST
                     AKSTTESSAKVVAGGDLQDGTYKLEEKNYSNGYRAVFEMVVKDGKITESKYDNVNENG
                     ESKTKDAEYNKNMEAKSGTNPEKYIPELNDQFLKAQSASGVEVVTGATHSSESFQNYA
                     QQLIQAAQAGNTDTIEIDNGADLKDGTYKLEEKNYSNGYRVQFEMTVAGGKVTESNFD
                     YIDKDGKSKQDDTEYNENMKAKSGTEPKTYIPTLNDEFVKAMGEEDGSPANVEVVTGA
                     THSSHSFIMYAQQLVNAAEKGDTQTIEVDNIVTEK"
     gene            44624..45727
                     /locus_tag="IV334_000661"
     CDS             44624..45727
                     /locus_tag="IV334_000661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HAP8942755.1"
                     /translation="MRKKLLFSLITLGISFMVLTGCSNSANKTTTESSAATKILKDPY
                     SDEQFLLGTYVRIRVYDEGKESALKPAFDRVKELGDKITINQKGSEIDEVNEQAGIKP
                     VKVSDDVYTLVKRAYEYSQDSQGGFDMAIGAITQLWRIGFDDARKPSQEEIDQALKLV
                     DYHKIELNDKEKTVYLKEKGMIIDLGAIAKGYITDEVVKVLKKQGVTTAIVDLGGNVY
                     VLGHSPRGENQDWTVGIQDPNMARGSVLGSIKERNKTLVTSGIYERYLEVDGKKYHHL
                     FDPKTGYPFDNDIASVTIITDKSIDGDGLSTAVFSMGVKKGLEYVESELNNGTEAIFV
                     TKDDKVYVTDPIKDTFKLSEDSHYTMGDRSELK"
     gene            45740..>45918
                     /locus_tag="IV334_000662"
     CDS             45740..>45918
                     /locus_tag="IV334_000662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoate
                     polyprenyltransferase"
                     /protein_id="HAP8942756.1"
                     /translation="MTLKIFLEVVEIRTKVASVFPFAVGVLFSIAFFHEIHWLNTFLF
                     FIGMLVFDLATTAIN"
BASE COUNT        13557 a         9605 c         8395 g        14361 t
ORIGIN      
        1 acgtttatta ctgaactcat tccttagagc taaactaatg agaaagagca gaataactga
       61 tacaaaaagc aatcctacaa gaattaatag actcatctaa atcattcacc ttttctaaaa
      121 taatccgaga aagatttaaa aaaattatat ccagctatta ggataagtcc tatcataatc
      181 actccaatag ccattttctt atcctcccta aaaataaagt cttttactta aaaccttttt
      241 tgtaatcgtc aataaatttt ttccctttat cagttaattg aaatcctata tctaaatagt
      301 cctcatatct aagtttagcc agcatctcac ttacagatgc tggactaaca cttagaactt
      361 ttgctaaatc attatttttt aatttctttt ttctttttga taattcctga attgctagta
      421 aataaattag tatagtagtc gttttaattt gtaccatacc taccctccaa attatttcga
      481 aaggccttca gcaattttat ccaggttcca tttcatcatc gcatagtaac tatcaccatc
      541 ttgtcctttt tctgcaattg aatcagtaaa aatcgttgaa tagataggaa tattggtatc
      601 ttttgatact gttttcattg gtctatcatc cacactgctt tctacgaata aggagggaac
      661 ttttgttgtg cgtaattttt caactaagtg ttttatttga tctggtgttc cttcttcttc
      721 agtgttgatt tcccaaatgt aagcagaagg cacattatac gctttcgaga aatatttaaa
      781 gcatccttca cttgtaacaa tcattttttt atcattcgga attgaagcaa atttagattt
      841 agcttctttg tctagagaat ccaatttttc aatatactta tctagatttt ctttatagaa
      901 ttttttatta tcaggatctt tttccgctaa ctgtttttca atatttttag cgtaaataat
      961 accgttttct aaatttaacc aagcatgagg atcttccttc cctttctcac tctgaccctc
     1021 taagtaaata acatctatgc catcacttgc tgcaaaatag tctttgtttt cctctttgtt
     1081 cgcattttta actaattttg taaaccaagc atttcctcca gtttccaagt taacaccgtt
     1141 ataaaaaatc aaatctgctt ttgaagtttt ttgaacatct tcaggcaaag gttcatattc
     1201 gtggggatct ttcccaatag gtacgatact gtgtaaatca attttatctt ttgctatatt
     1261 ttcggtaata tctgctaaaa tcgaattagt aactactact tttaattttt cgttcgaagc
     1321 cactttatct ttgtctttac tatttattga acatgccccg aatataaaaa gagcaatcaa
     1381 aatccccaga cttcccagta ctgctttcca ttttctcatc ataacgatct ctccttcttt
     1441 cttaaaaatt tttgttttgg tgagattaga aaactaatta caaataagaa tgccgctgtt
     1501 aaaacaatac ttgatcctgc tgctatgtta aaactataac caataaacaa tccaagcaat
     1561 gatgaacttg ctccgactgt tgcagaaata aatatcatag ttttcaagct atttgcatat
     1621 aaatatgctg tagctgctgg tgtaattaac attgcaacaa ttaatatagt tcccacactt
     1681 tgcatagctg ttactgctac taaagttaat agaaccatca atagataatg ataaaaactg
     1741 acattcatcc ctattgcttt tgccattaat ggatcaaaag aagtgattaa taattcttta
     1801 aaaaatagaa ctactataac caacactaaa accgaaacgc caattgtaat ccacttatca
     1861 atatcttgta cagctaaaat atttccaaat aaaatatgaa ataaatccgt tgagctattt
     1921 gcaactccta taaggataac tcctagtgct aaaaaagaac tgaaagtaat accaattgct
     1981 gtatctcctt ttattacact attgcttttt ataaaagtaa taatgataga agctaataga
     2041 ccaaatacaa tcgcaccaat aaaaaaatta atacctaaaa tataagaaat tgctactcct
     2101 ggcaaaacag catgagaaat agcatcaccc ataagagaca ttcctcttaa aataataaaa
     2161 caacctactg ttccagaaac aatccctata actacagatg taattagagc attctgtaaa
     2221 aagtgaaagt catacagacc attaataaaa ttttgaagca tattattccc cctctccaat
     2281 aaatatagta tctccatagg ctttttttaa attttcttca ttaaatacag attctacact
     2341 tccgtgagca attagatttc gattcaaaag aataatatta tcaaaatact tctttacctt
     2401 gcttaaatca tgatgcacaa ttagcactgt ttttccctga tgtttcaatt tttttaacgt
     2461 atccataatt attcgttcac taactaagtc tatccctaca aatggttcat ctaaaaatat
     2521 gaaatccgca ttttgagcaa gacatcttgc taaaagtaca cgttgaaatt gtcctccaga
     2581 taattctcct atttgatgat tagaatattt tcccatatcg accttttcca aagcctttga
     2641 aactctttgc cattctgcat ttttaattct ttgaaaaact ttcattccgg catatgttcc
     2701 catagataca cattctttta cttttattgg aaaagtaaaa tcaatatcag ctttttgctc
     2761 tacatatgca atcttcacca gttttttatt gatatcttca ttattaatgg agacctctcc
     2821 ctgatgaggg ataatattta acattgcttt aagcaatgtg gattttccag caccatttgg
     2881 accaataata cctgttaaag caccttcttg tatttttaaa gaaatctcgt tcaatgctaa
     2941 aaaatcgtta taacaaacat taagcttttt cacttctaac ataactttct ccttctaaat
     3001 gataatgatt atcacgatca tatggtatac aaaaattata ttttagtcaa ccctaaattt
     3061 tatagtttag atttttttct gttgctgaag attagaatga caaaactatt tgaaactacg
     3121 cgtatagtaa gtagctttca aaatatataa aaaaggagcg ttctcaaatg agaacatgct
     3181 cctaaaacta ctttattggc gtatggaaaa attatacttg aagtggatag cgggttgaat
     3241 aagttgataa gcatcaaaac atttataaaa attattcaag actatgtctg ttccatatac
     3301 aaatatgaaa atcagtaaac tccatttaac ttttttcgta gtgtgtcttt ttatacatgt
     3361 ttatcgttat taatagtgaa taggaaaaaa actatttttg atcattgaca attaaataca
     3421 atttttgaat aacgatccct gctattcgag ctagaaactc aagtacgcca tgacctgatt
     3481 agcgacaaat acttagagat ttctttttgt tctgggaaaa caaaatgcca tgacaatagc
     3541 agcaaataat gatacttccg taaggctcgc cgtaaactgg ggagaggtga aattcctatg
     3601 tgcttaccca aagcaattca aaaatttcat aactactatt ttaggagatg attttataga
     3661 atccagagtc tacctgaata aaaaacaaac ctgtgcattt ctaggaatcg ataataatac
     3721 tctagataag tggattcgta tatataattt cccagtaata agagttggtg gtgtagttcg
     3781 ctttgacgta gaagatatta aagactttat gaatacattt aaaataactt gaaattttct
     3841 tctcgccgca tatactaaaa ttagtgaaat atactgcctt acttttttta aggagtgagg
     3901 attttgccta caaagctttc aaatgggaaa tataaaacta atctgagata tccaaaaagg
     3961 tttaaagaaa taacaggaat tgcatcagaa aagtatcaaa aaacatttcc aaataggcaa
     4021 ttggcgatta aagccgaaaa tgatatgaag aagaaaatag aaaaagtttt gagagaggag
     4081 aatgcaaact cattagaatt aaaaggaaaa ataacgttta aaaaatttta cgaatctaaa
     4141 tggcttccta gatatgaatt aggtcaaaca attagatcaa acaggcctcc tagtgatata
     4201 accattagta atacaaaaga tatatttcgg ttgcatattt tacctatgtt tggtgaatac
     4261 gcaatgaatt atctaaatat caatactgag ataatatcag atgaattaac caaaaaatcg
     4321 aaagaatatg caaatataaa aatcataaaa ggatatgttc gttccatgtt cgatattgca
     4381 gaaatactaa attatatcga atttaatcga actactaaaa ttattcaaag tatcactgca
     4441 cctaaaaaaa atgctttaga agaaaaaaga atacaagaag gtaagcaagc actatcatca
     4501 aaagaactta ctaattggat agaagcagta aatgatgatt ttaataatca tttacttact
     4561 tttcatgatt acacactttt tatgattacc ctgtatcttg gtgatagaaa aagtgagact
     4621 tacgctttgc aatggaaata tattgatttt gaaaaacaaa cggtccgtct taaacatact
     4681 ttagataaat atcaaagaaa aaagtttaca aaaggtcgaa aagataccgt cattcaagta
     4741 ccagaagttg taatgacatt gctaagtgaa tggaaatcgg tacaagctga tcaattgttg
     4801 aaattaaaaa ttaagcaaac gttagatcag tatctcttta cttatactaa accatctgga
     4861 gaagtaaatt gtcctgttca tgccgattac ctaaactatc gaatcaattc aattaaaaga
     4921 aggcatcctg acttagttca tttatctcca cacaaactcc gtcatactta tgcaactata
     4981 gctagacaag gtggagcaga tatgaatcaa atttcaaatg ctttaacaca ctcagatatt
     5041 tctacaacaa agatatatgt gaacacacca gatatagttg ataaggctgt ttttgaagct
     5101 tttcagagag gattgaataa gtgtgattga tatgtgttct taaatgtgtt cttaaaaaca
     5161 aaagaagcac tttccaaata attgaaaagt gccagaaacg ttgatatata aaggatatta
     5221 acgttttgag aattgtgaag ctttacgtgc tttcttaaga cctggttttt tacgttcgac
     5281 catacgtgcg tcacgagtaa gtaagccagc acgtttaaga gctaaacgga agtcaggatc
     5341 aacttctagc aatgcacgag cgataccatg acggatagct cctgattgtc cagcgtatcc
     5401 tccgccgttt acgttaacaa atacgtcata tgcgcctttt gtttcagtaa cagcgaaagg
     5461 ttgattgatt acttcacgca agtcagcatg tggaatgtat tcttcaacat cttttttatt
     5521 tacagtaatt ttgccagttc ctggtactag acgtacgcga gcaactgcgt ttttacgacg
     5581 gcctgtgccg atatattgaa cttgtgccaa tgaaattccc tcctattaga ttaagtttgt
     5641 gatgtctaaa acttctggtt gttgtgctgc atgtggatgt tctgctccac cgtatacgtt
     5701 caattttgta aattgtttgc ggcctaaagt gtttttagga agcatacctt ttacagaagt
     5761 ttcgatcaaa cgacgagaat ttttagcacg caattcgcca gcagtgattg atttcaatcc
     5821 tcctgggtag ttgctgtgac ggtaataaat tttgtcagtc gcttttttac ctgttaattt
     5881 cactttgtct gcattgatga cgatgacgaa atcgccagta tccaaatgag gtgtgaaagt
     5941 tggtttattt ttaccacgta gtacagatgc aacaactgtt gaaagacgtc ccaatggaac
     6001 atcagttgcg tctactacat accatttacg ttctacttcg cctggtttgg ccatatatgt
     6061 tgtacgcacg ttattttcct ccaatttaag ttctgatgtt tgacatacac ttcgagtttc
     6121 cggggctctt tgtggggcaa acaataccat ttaacatatt acccgctaat ctagtcaatg
     6181 tcaacgtttt tctgcttgat tgaaaaattt atatgaaact ttacttcttt tctttctttt
     6241 atataaaagg gtcgtttcct gtcaaaatga aaaacaaaaa gaggctgtga caaaagtctt
     6301 gtcacaaccc cttattttga ataaacggtg ttctcctgcc aactcctcgg tcacaagtca
     6361 caatatccat aaaacataag aagctgtttt ttaaaattgt ttcttgtgac tcggagctga
     6421 acggcaggct tacaacctct tggtaaacgg tgttcaatca gctgaccctc ggtattagct
     6481 caaaaaacgg caaaatatga ggaactattt tgacgtttgt ttctctaata ctcaggtcaa
     6541 aacgctgatt tcacaacctc tttttcggtc aagttcaaca acagttttta cgcgtcgatc
     6601 tctggcgctt tgcccaaatg agcttggatc atccagatac gtttttctgt ggctgttttg
     6661 aattcgataa agatatcttg tgttggatcg tcgccttctt ctccagaaac ttcgattcct
     6721 ttttggtaaa gatctgctag ataacgatag cctgcaacaa gtgtttccaa acgttctgga
     6781 atagtacgat cccagcggcc aatttcatca gggatttttg tgttatctgc aaattcacgc
     6841 aatgtagaat aaggtgaacc gtctaatgtg atcaagcgtt cggatacttc atctaactga
     6901 gcgttcaaat catccatgaa ctcatccatc attgggtgca atgtaaggaa acctggtcct
     6961 ctcatgtacc aatgtgtttg gtgaacaaca acagacattt ggcttagatc tgcaactaat
     7021 tgatttaata cttctttggt ttgttggaat ttcatctttc ttgcctccta acattttctt
     7081 acacttaaaa taatagtaga ctttcaatga aaagtacaac aaaatcattt acctgagaac
     7141 gattttcagt tcgctttttt atccgtaaag ccaatcattt ttcagaatca gctaattctt
     7201 ctcgcagttg agcaaattgc tgataagtct gtgctaaatg cttttgtgtc gaaacaaata
     7261 atggatagat tttctgatat tttttatgat cagtttcttg tactccaact tgctctttca
     7321 attctatcaa ttcctgtgct tcactgatac tttggatctt tccgatgctt ctccagccta
     7381 aaatcacagc tcctaatgcg gatgcttctg tgacagtagg gaaaacaagc gggtaaccca
     7441 gtacatcagc catcatctga cgaaaaatcg gagattgagc aaacccgcct gtagcacgga
     7501 tctctgtgag ctgattttcc tggcaaactc cttttaaaat ccgataaagg ttgagacaga
     7561 tcccttctag aatcgccttc atcatttctt tttctgtatg ttgccgctgt aaaccgataa
     7621 aactgccaaa cgcatctgcg tcccaaagcg gtgctcgctc accaagtaaa tagggatgaa
     7681 aaatcagacc attggcaccc gctggcacaa aagcgatagc cgaaagcagt gcatcatatg
     7741 gattttctcc tttttcttta gcagattgaa cgacttcctg taaaaatgta tgccctgccc
     7801 aatcaaagat gccagcacca ttgctagtcg ctcctcccac tacccaatgt ttctgatcca
     7861 gaacataaca aaacgtctcc atttctggat ggaactgagg ctgagtagta ataaaacgaa
     7921 gtgctccgct cgttcctacg gtcaaagcgg cgatcgtatc accaaaagca cctaacccta
     7981 gattagaaag cggcccgtct gcccctccca aaacaaatga tatatcattt ggcaaaccta
     8041 gttctgctgc tgtttttgat ggtagacctg ttacttgata ggtggaagga aagacttgcg
     8101 gcaattgctc tattcgaatg ttcaagtagt caagcacttc ttctgcccat tggaattcat
     8161 ggatatcaaa atatccagtt cctgaagctg ttccgtagtc gctgatcagt tcgccaaaca
     8221 atctataaaa gatatagtct ttgataccga caaaataagc agtttttttg aataattcag
     8281 gttggctgtt ttgcagccac ttgattttgt tcaatggtgt cattggatga atcggcgtcc
     8341 cagtctgccg ataaaattct ttgccaagca cggtatattt cagtttttct gcttcgtccg
     8401 ctgctcggtt atctgcccaa gtgatcattc gagataacgg tgttttctct tcatccaaca
     8461 atatcaggcc atgcatcgca gaggagaagg agacaaattg aatgattgtg tctgaaggca
     8521 actgtttaat tacttctttt ataacggtgc agacaccttg aaagatcaat tcctgatctt
     8581 gttctgccat cccttgtgtt tcttggatca aagggtagcg aaaataagcg gatgctgctt
     8641 cttgggcttc atctgtgaaa gcaactgctt tggtttgtgt ggttccgata tctaatccga
     8701 ttgaaacgtt cattttttca cctcaaattt tatgtatgta caaaaattta gtttaagctt
     8761 gcgtatcttg gttttgaaaa gggggagatg gcgtgttact ttattttatc attggttgct
     8821 gtatcggatc atttttatgt ttaaccgccc agcggattcc gttaggtcac tctattattt
     8881 atcctagatc tcattgtgtc aaatgctgtc gttcgctttc ttggtatgaa ttgattccga
     8941 ttctctcgat catcgtccaa cgatttcgct gccgctattg caggtgtcgg ttgcccttct
     9001 actatttgtt ggcagaagcc ttatgtggtg gtctttttgc ctggttcttt actttttctt
     9061 ccgcaagaaa tttttcgacg ttcatatgga tgctttcggc tttgctattt tcgttgatgg
     9121 atttatttta cttcatggtc cattctcaca cgctattctg ttcttgggca attttatgga
     9181 ttttttggtt gcaggccggc gtcttccaat ggcaaagtgt gctgttaacg tttatcgtag
     9241 gcggtgtgtg ccttcgctat ggtcaatcct atctcggagc tggcgatact ctgttgatgc
     9301 tttcttggag tggtggcttg tctctagaag agttgcttca gatcttgttt cttgctagtc
     9361 tgtttggaat atccgttttt tctgtttact ttatctgtca taaaaaaaag ttggaaaagc
     9421 tgcctttcat tccttttttg agcagtgcgt tgctgattgt ccttcatctt aggtattgaa
     9481 aaaaggttgt gacaaacgac ttgtcacaac ctctgatata tagtgttctc ttaccaaatc
     9541 cttgtgactt ggagctaggc gacaggttca caacctctcc actaaatggt gttcaatcag
     9601 ctgaccctca gtattagctc aaaatgctgg ttttacaacc tctgatatac ggtgttccat
     9661 aagtagcttt ttcggcttct tatttcttga cgccccaatg tcgacgaagg aaacctgcac
     9721 gtccagaacc ttctcgataa agattgtaat ggagcttgtt tttcttgtaa taatcctgat
     9781 ggtaatcttc tgcaggataa aattcgcttc ttggttcgat ttttgtcacg attggcttat
     9841 cgaatcgccc gctgtcttgc aatgcttgtt ttgaacgctc tgcaatattt cgttcttctt
     9901 catcattata gtagatcact ggacgataag aatcaccgcg atcagcgaac tgtcctaatg
     9961 cgtcagtcgg atcggtttgc tgccaataga tttccaccaa ctgatcataa gaaataactt
    10021 ctggatcata ttcgatctct actgcttctg tatgccctgt tgtgcctgtt gttacttgtt
    10081 cataagttgg atttggtaca tgcccgccag tataaccgga tgtcacagaa atgatccctg
    10141 gctgctcttc aaatggtttg accatacacc aaaagcatcc gcctgcgaaa actgcttttt
    10201 ttgtcatata aaatccccct tttttctcat cataccttga tttccaaaag gaggataacg
    10261 ttttgcttgt gacagaaaaa acggaaacta ttatatacgt gatacggctt ctgataaatg
    10321 ccaaaacgaa cgtacttgat caaaaacggt taaatcagga atcacatatt cagcagaagg
    10381 aagatttatg ttttcaccta ttcccaatac atgcatgtta ccagccaaag ctgcttgaca
    10441 accagcttct gaatcttcaa tgaccaagca tgcatttggc ggtacattca attgctgtgc
    10501 tcctttgaga aaaacttctg gatcaggttt tgcttttgat acttgcgtgc cgtcaataag
    10561 gacatcaaaa taacttgtca aattcgtttt ttctaaaatg aggcgtgcat ttttgcttgc
    10621 tgatcccaat ccgatcttga tgtttgtttt tttcaagtac tcaagaacag ctattgcacc
    10681 aggcaaaacg gatgtttcat ccattttttg aatcgcttga agatatagat tgtttttttg
    10741 ctcagcaaag gctgatttct ctttttctga gaatttctgt tccatttttc caaatgacag
    10801 caatcgctct agtgactcgg ttctggatat tcctttcagt tgctcgtttt ctttctcagt
    10861 gaatggaatg gacagctggt tagctaaaac cagccacgct tcgtaatgat aacgagctgt
    10921 gtctacaaga acgccatcta aatcgaatag tgctgctttg attttcatcg attacctcct
    10981 tgaaaatgaa cgacttgaag taaagatgcg atgtttttag ctgtgcgatg aagattatca
    11041 gtggcttgct gtaaagcttc ttgtaaaggc acagctgcag gaaggatcgg aaaaacggcc
    11101 gaaatgccaa catctgctgc tttttcgctt cctttaccaa cacttccgca aatagctatc
    11161 acaggaacgc cctctggtac tcttctagca atcccaactg gcgcttttcc ttttagactt
    11221 tgtccatcta gacgtccttc gcctacgatt acgaggtctg cttgctgtac ccgtttatcg
    11281 aactccagta aatccaatac gtagtcgatg ccagagacta ttttccctga agcaaatgct
    11341 aacagtcctg cacccattcc accaccagca ctactgcccg atgcctcgat tacttgaggg
    11401 gcaaatctca tataaaaatc atgcatggct tggtcagcca ctgctaattc attttctttc
    11461 aagccttttt gtggtccgaa aacaaaagaa gcgccttctt ttccgcataa aagattagta
    11521 acatcagtaa tgacagtgat tttggcttta atcagctcaa taggcacgtt cgttttatca
    11581 actgtagcaa ttttacctag attttctccc agtgcttcta ctttttgatt atcttgatcg
    11641 taaaaagcat aacctagacc ttctgccatg ccgatgccgc catcgttggt actacttccg
    11701 ccaacgccga tgatgatgtg tcgaacacct tgttgcaaca attgtcgtat cagttctcct
    11761 actcctcgcg tggataacct aagtggctgt tgttggttta gtgggacatc tttcaatccg
    11821 acgatttctg ccatttcaaa aatcgcagtt tgaccgtctg aagcgtactg gatttctttg
    11881 tttttgccaa atgcattagt aacgactgct gtttgccgct tcaaccttaa actgtctgct
    11941 aatgatgaaa ccgttccttc tcctccatcg cctataggta gcagatcata ttcagcctga
    12001 acaaaaactt cttgaaaacc tgattgaatg gcttgtgctg cctctaaggc tgtcagactt
    12061 tctttgaaag aatctggcgc aatcagaata cgcattattt tcccctccgt tccaagatca
    12121 gttctttttc cagctggtat tggtctccat tgacaaaaat aggtaatgtt tctttcgtct
    12181 ctttttttat tactatttgt ttttttgtca aaacaaatag caacctgtga tttttccaaa
    12241 taaaaggaaa agaaattttt tcccatgctg aaggaagtct tggagaaata gaaagagttc
    12301 cattcgttac tgtaatacct gaaaaaccaa atataactgc tagccatagt gctcctaatg
    12361 aagcagcatg aattccgtca tctgatgaat gcggatctgg tcctaaatca atcaggcacg
    12421 cttcttggaa taaatgatac gcccattcca tttcttggat acggcaagcc tctatggcgt
    12481 gaatcgcttt actcaacgat gagtcgtgga ttgttttgtt ttcatagtaa tgcaaattct
    12541 tagaaacgat tggttgagaa aaatactcag gtaataagta taacagcatg acaagatctg
    12601 cttgcttgct gacttggagt tcatttactt cgctacgggc ataatcaagt aaaatagact
    12661 ggctgccagg acgttctttg taagtggtca aatcaataac tggcttactg agatacgtgt
    12721 catcttgcgg aatcagctgt tctttgttag gcactggcaa atataactta tctaaaaaat
    12781 tttgacattc tttcaaaaaa agcggatcgc cagcttgata tttaacagct aaggccacat
    12841 tataatgcgc cagatagttc gtatatgcat tattgtcgat atattctgtg tattcatctg
    12901 gaccaatgac tctgtgtatc tctaaatgat catcgacttt tacagctctg ctcagccaaa
    12961 aagctgctgt ttcatgaatg agcgtttttc cctttgtttc taaaaacgat tgatcctctg
    13021 ttgcttggac atactcttga acggcataag caaggtctgc actgatatga tgttctgcgg
    13081 cagctgaagc aacttcttgt cttgttccgg ttttgatatt caatgcagca aatttgggtg
    13141 tctcttcctg accagtgtgt gcgctttccc atgggaaaag tgcaccttga tagtgataat
    13201 ctcttgcttt ttgccgtgcg ccttctaatc gatcataacg gtacagcagc aaattctttg
    13261 ctacttcagg aaactgatat aaaaagaaag gcaggataaa aatttccgta tcccagaaaa
    13321 catgcccttt ataaccttcg ccagttagtc ccttcgcccc tacactaaca cttggatcat
    13381 tgcggggagt cataatctgt aaatggtaag cagcgaagtc catcgctaac tgatcgaaag
    13441 aagaatcgcc ttggatcgtt atccgatgct gatgccaaaa atctgtccaa taagacatcg
    13501 actgctctaa aatttctgta taactaacgc ttttctgggc ttgggcaata cttttcttaa
    13561 acgggtctgt ttgctgaaga gaggtagaga gcacgatgat cttctccagc gtaaaggacg
    13621 tattttttct gatcttttct gaaaaggaag cggtgatccg gcgatttttt gcttggaata
    13681 cacctgcttt attgaattta gctgcaatcc caatatcaat tttactttct gttgtctggt
    13741 aggatgcact gatcaaatcg tcaccaaata ctcgcagttc tttttcaacc aactgctgtg
    13801 taccaaaatt ggtttgctgt ccatcgatac ccgttttcac atctacttgg cagtcttcgg
    13861 tcaacggctt gatgaccact ttcagggctg cctgatggag attattttga tcagcaacac
    13921 gacaagtaat gacttccact ttttttcctt gagcagtttc aatgagcgca tagcgtttaa
    13981 gctctcccat ttctaaatct agtgcccgct cgtatgcaag aactttcatc gtctcaagag
    14041 aaaaatgttc accgtcgatt tttatttccc atctgatgac atctgggaga ttcactaact
    14101 cggctgattc tgctcctaaa ggtcggttat atacaccagc tgcaaacatt ccccttactt
    14161 gattccgata gtcttcctcc tggcttcctc tgataccaaa aaatccattt ccaatggtca
    14221 tctgactcgc atacttattg atatactcta aatcaaactg tttttcttct actttcataa
    14281 ggtaataact tctcctgttt ctgccgaacg atataaattt tccaccagat tctggatgac
    14341 atatccttgc cagccatctg caatcatctg agtctggtca ccgagcgccc gattcacaaa
    14401 atgagccata cttagttcat gtgggtcatt aactggcgtg gtcttttgta aggaaaccag
    14461 ctttccatct acatcttcga atagttcgcc tggatacaga ttagcacctg cctgatcgcc
    14521 acaaaaagcc acattcagtt tgtcttctct gatattcaaa gcaaacgaag tattcagttg
    14581 gatgatcttg ccgttttcta attccagtgt gccaaaaatt gcatcttcta ctgtgaattt
    14641 gcttggatcc catttcccga atttccctga attttttttc gtaccaattt tttggaagct
    14701 gtacgcccga acgcttttta ctttaggaaa tccagtaata tacatcgctg tatcaagcat
    14761 gtgaatgcca taatcgatca aaggcccgcc tccttgaagt tctttgtcga taaacgttcc
    14821 ccagcctgga acaccagacc gacgaagcgc attcgcttct acgtaataaa tatccccgag
    14881 aacatcaaga tgattcttga tcattgttgc ttcgtcagaa aaacgatgct ggaatccata
    14941 agccaaaatc tgcccgtttt tatcggctgt ctctgccatc tttcttgctt gatcagctgt
    15001 catagctggt ggtttctcgc acatcactga agcattagct tcgagggcag ccaatacatg
    15061 ttcgaagtga aagcgattgg gtgtacagac actcacgata tcgggagaaa tctcattcag
    15121 catttgtgta actgatgtat acgcatgaga aaatcctgta cgtcggcaaa agtcttctgc
    15181 tcgctggctg actggatcaa ccacagcggc caattctaag acatctgatc ttttttgata
    15241 ataagcagca tgtacttttt ctgctacttg tccagcgcct atgattgcta ctttcttcat
    15301 ttatttctct ctcctttgct tatttactta ttgttattct ttctctattc tttctctaaa
    15361 tcatttaaat gacgcgctgt ttccagacag tttttcaagt attgcaatga ttcctgataa
    15421 attttcactt cattttctcc tcgcaaacga cattcatata ccatataacc ttcatactca
    15481 ttttgaagta gcgtttgaaa atggcgagta aaatcaattg cgccagatcc tggttggtag
    15541 cgatgattat ctgctaaatg gatatgaccc acatactctt tttgcgtagt caatgctttt
    15601 gaaatatcat cttcttcaat attcatatga taaaaatcag cgatgatctg cacatgtttc
    15661 aattgattag agtcaatgta atgacgcgca tccgctaaca aattgatcat atggtcttgg
    15721 tatcggttca aaggctccaa ataaataacc gtacctgttt cttcggctac tttatctaaa
    15781 taacgaagtg attcgctgac tgctttatag tctccttctt tgcttcgggg tgaaaccatc
    15841 ggcggtaatc gataagtgaa cattccccaa gctgctggga taacgattcc tttgcctcca
    15901 acctcgctta gtgcacgcaa aatctcagca atttgacgta caccattaag acgccgttct
    15961 tcgataaagt caccgatcca cccatcgtag ccgttacaag ctgtcactac tgggatccct
    16021 gtttctttga ttgcctgttt gactgtgtcg atattttcta ttaacaactt accgtcgatt
    16081 tcgtattctt caaatccaca gtctttgata aatctaaatt tttctaaaat gtctcttggg
    16141 aaaaatgctt gatcttgcgt accgaatttc attgttctcc tcctcggact tgaacgccca
    16201 ttttgatact ttcttctggg tgctggtcta cataacgcat gtaagcttct gcgctttctg
    16261 caaatggcac aactggatca atgatttctt cacaatccaa gtaaccgttc atcaataatt
    16321 cccaacatgt gtcctcgatt cgtttacggt tccatcttgg gtaatctggg tttggttcac
    16381 tagatgctcg agcaaagacg atttttgcat tattgaaatg tgcttcgcga ccaagattaa
    16441 acccagctgg aaaaggttta gcaaaggcaa cataagaaat gatcccgcca tagccgatcc
    16501 ctattaaaga agcttgtaat gcggcagcac tgccactcgt ttcaataact gaatctactc
    16561 cacgtttatc tgtcaattcc tttagacgaa aaccgacatc tgctttcata ggatctagcg
    16621 tgtaatcggc accgtgtttt tctgctaatt ctctacgctg cgctatggga tcgatcccaa
    16681 cgatggtagt agcacctgct tttttagcta attgaatcgc aatttgcccg atagcgccta
    16741 aaccgaagat tgctacagca tcacctgctc gaacatgtgc ttcccggata ccacctaaag
    16801 cgaattgagc aggatcgtaa catactgcat ttttccattg atccaaccga tcaagtttac
    16861 gtagtttata attatcaaca ccgtttgtaa tcagcgtttc acgaatgggt ccataagtgc
    16921 agactaactc tcctttttga tactctgtca ccaaactgcc cgtttcttcg atcgtaccga
    16981 caatcatatt tcctaattga aattcgccaa ataccacacc gcgttggctt ccttcttctc
    17041 ttggcaagaa taggcgccat tcttctgaaa attcttcatc gataaatgga cttaatcctc
    17101 taaagtcgac aatttctgtt cctggttttg gagtagcgaa aattactcgg atacgtactt
    17161 cgttcttttc gatctcacgg tcttcgtaat ctttgaatcc ggctattctt ggttctaatg
    17221 caactaattt tttcatcttt tctttcctct ttcttttctt tttatccttt tacgccgcct
    17281 tcgactaaat cacctttaat aaatcgttca gataacgcat acataatgat gactggtaaa
    17341 gcggtcacca atgaagcggc catcattcgt ccccaaatgt agtctggtgt actgaataat
    17401 gtgttcaaac cgattggtaa agtaaatttt tcggaggtcg acaggaaaat cgaagcaaat
    17461 aaataatcat tccacgaaac cataaagcaa taaacgaaga cagaaatgat tccagaaata
    17521 gccaatggta taatgatccg aataatgatc tggatccggc taagaccatc gatcattgct
    17581 gcttcttcaa tatcagtcgg aatcgtgtca aaataacttt ttagcatgaa tatcgcagta
    17641 ggaagtgttt gaacgaccat cgtgataatc agtgctgtat tggtatcata caaacctaga
    17701 ccggaaatga ttttaaataa tggaacaacg atcaaaatac cggaaaacat ataaacggtg
    17761 taaaagctag cattgatttt cgagcgagct ttgaagcgta gttttgacaa agcgtatgcc
    17821 ccgctgatcc caatcactac tgcagttacg gaggctatca aggagatcac taaactattt
    17881 ttgaaataca tgatgaatgg aaaaatctca gcattgaaaa tatcctgata atgttcaaaa
    17941 gtccattctt tcggaaaaat agtttgtgtc gttgcaattg cttcttgtga atccttgaat
    18001 gatgtcatca gcatgatcaa aaatggaaac aaattgacag ctaatagcaa caaaatcgtc
    18061 gcgtaaaata agattttcct gctcttttta tttttcattt caagtccacc ttctttcgtg
    18121 agagcaagac gaccgctgca ataatgatga acaatagaat cgatatagct gctgcttttc
    18181 caagatcatt gaacgcaaag gctgtctgat acaaataaac gcctaagata ttgactttat
    18241 tcgtcagtag atagacatct gtaaacatat agaacatcca gatagatcgt aaaacaatca
    18301 cagttgctag tactggtttg attgccggca gtgtcacgat cttgaacttc tgccaagcat
    18361 ttgcaccgtc catttcagct gcttcataaa gcgttttgtc gactgtttgc aaaatagcca
    18421 aaaatgaaat aaacgcatac ggatagtatc tccagatagc aaacaaaacg accataaaga
    18481 agctagcaac tggctggtcg aaccacagcg gtgcttcgtt gaaaagctgc aacctatcta
    18541 ctaacagatc attgacgatt ccataactat tattgaacat atatttccaa gcaaaaatca
    18601 aagaaataga tggcgtaaca taacttaata aaatcagtga acgcaccgtt ttcctaaatc
    18661 tgaacttgcg gttaagcaaa atagctacgg ctaatcctaa agctgtactg cctgctacaa
    18721 ctaagaatgt gtaagcaact gttaaaccta aagaacgata aaattcacta tcacttaaaa
    18781 tttcaagata attgcttaat ccgataaaaa cggcgtctga gttaggattc agcggcatat
    18841 cgaaaaaact gatctgaata ttggaaagca ttggaatcag cactaaaaca gctaataaaa
    18901 gcatgcttgg tgctaaaagt cccatggcta attttaagtc agaatgtttc cttggacgat
    18961 tcattgaaac ctccccctga taatctgcca atagctatct acttttttcg aacaccattg
    19021 gcagaacttc ttgttatttc gtattttttt gtgcgtcagc taaagcttga tcgacatctt
    19081 cgtttccgac agttacttgg ttcactgctt ttccgatcac acctgagctt gtgatatctc
    19141 ccattttcgt gaaattcttt tccccgacta gtccaaagac ttgtacttca tcaaaagcac
    19201 tagcaatctc agatggcagc tcgccaaaag acttgatgac ttcattttct ttgtaaccat
    19261 ccagttcgac tacttgttta ttcactggtt gagcaccacc aggagacatc aagacccact
    19321 tttccatgtt tttcggttga gataagaaag ctgtgaatgc ttcagcagcc tctttttgtt
    19381 tctcgtctag accagaagaa atcgtcaatc cagatactgt gccatagaca gcttgatctt
    19441 tttgtgttgg gatagcaaaa ccgatatttt ctgagttccc cgcttcataa acagaaggca
    19501 aaatgtaagt tgaatagatt gccattggtg ctgttccatt catgaaagca tctttgattt
    19561 ctgtcacatc attagagcct ggcatcgtgt attgggataa gttttgataa aacgacaatg
    19621 cttctctcat cttttccgta tcgatcgtta cctcgccttc atcatccaaa acatttgctc
    19681 catttgacaa agcaaattga gagaacgctt gctcactcat tgttccttca gcagtaggca
    19741 tagcaattcc atattgttta ttctctttat ctgtgaaatg ttgagcaatt tttaaaatat
    19801 catcccaatt ttctggttct gaaaaaccag cttctgataa tttttctttg tcataccaaa
    19861 taccttgaac ccagccgcta attggtgcag caatatagct ttttccatct tctgaacgaa
    19921 caaggttttt ggctccgtca taataattat cttctccagc ttcctcgata acgttttgga
    19981 ctgccgtctg atcaatcagt tcatctttat ccatcacttt agcaaaatcc tgactgactt
    20041 ccattacttc tggtaattta ccggaacgag ctaacgtaac gatttttgta ttgaacgctg
    20101 attcttctac cggcacttgc ttgattttga tgtctggatt ttctttttca aaatcagcaa
    20161 ccagttcatt gatcacactt aatcgttcct gttcgacaga agagtgcatg aattgaatcg
    20221 ttgtctgttg tgattcgtta gagctttgac tttcaccaca agcagtaaga agaaatccag
    20281 tagctagcag acaggtagta acaataattt ttttcatttt tttccacctc ttattttatt
    20341 attttcagcc acatatattc atacggctgg aatatcaact ttcgttcttg actgcaatgg
    20401 ataatttgtc cagaaaacag gtctaggtaa tctccttttg gtaaaagata acctgctgat
    20461 tgattggaaa aattatggat aactaagact ttctcttcct tcgcttcgcg aataaaactg
    20521 ataaaagaat ctgctgagtc cagtaagtaa atcaaactat tcggatggaa ggctttttct
    20581 ttttttcgaa tagatagcat ctgggtcaat tcaccatata ctgctgttct taatggatct
    20641 gtctgcaact ctttctcaat atctgctaac aaaaattttt gccgattgat ttttcgattc
    20701 tctcctgttt tttctacgcc tttgtaatca tttcgacttc ccagcacact ttgaatataa
    20761 attgccggca cgcctggtaa cgttaccaaa aatgaatgag cagctaaaaa gcgtttgctt
    20821 ctccattccg tcgtatctgt tggagaactt aacgcatcta gatacgtagc gtttatctca
    20881 tatggactgg tcgttccatc tgggtttttc ttgtagttta ctcgggcacc ctctttcttc
    20941 agctgttcca ctaattcaag gatcgttttt tccggcagaa tacctcgtac cggattcaat
    21001 ccgataccgt catgggaggc taaaaaatta aaaaaagttg tttttttcga tgcgaaatcg
    21061 atggttttta accattcttt caacttactt gtattacccg aacgaatcgc ttgaagggtc
    21121 aaaggtggta gcgaaaactg atagaccatc tgtgcttcat tctcgccatt cccaaaataa
    21181 ctgatattat ctttgtgtgg cacatttgtc tcagtgatca ataccgttcc tggagctgct
    21241 tcatcagcga ttgcacggaa taattggatg attttatgcg tttctggcag gtgtatacaa
    21301 gaagttccta gttctttcca taaaaatccc acagcgtcca agcgaataaa tgaagcgcct
    21361 ttttctaagt aaaacaaaag aatatcgatc attttcaata aaacttctgg atttttaaaa
    21421 ttcaagtcta cttgatcttc gctaaaagtc gtccatacgt gtttttcttt cccgtttttg
    21481 tcaatgaacg ttgtaagtaa cgggctggtc cttggtctag tcacgctgct tgtatctgta
    21541 tccagtggca aatcaataaa aaaatctttg tacgtatcat tcaagtttaa atattcctga
    21601 aaccaagtac tttcggcaga catatgattg cagacaaagt cgaacattaa ccgtgtttcg
    21661 cttctcattt cttctatatc ttcccatgtg cccaaacggt cgttgattgc ctgataattt
    21721 acaacggaga aaccatcatc agatgtatat gggaaaaatg gcaagaaatg tacaactcta
    21781 aatgtatctg ccaaatactt ttgataaaat ttcttaaatg tacgcaaagt tggttgctgc
    21841 tcttcaaaaa attgatcccc ataagtaatc aagaaaatat cttcttcgtc ccaatgcagt
    21901 ttgcctgcct ggcctttctt ctgctgataa acactgactt tttgttcaat catagaggcg
    21961 gttttctcag acaaatctga gtcgcgataa atcgcttgta aaagttttgt atacatcgga
    22021 ttcccctttc ttttttggga gcgttcccaa cgatttgatc atattatgga aaacgcttac
    22081 tgtcaatagc atttttttaa ataattttga tatacacaaa aatacaaata agaaagctta
    22141 tgtatcaata attaatagct tcttaaaaaa atatggtctc tcatgatcat gcaagctatt
    22201 cctaaatttt caaaatcata gacaaaaaag aatgtctatt gtactatgga attattctaa
    22261 aacgctccca aaaaggaggt gcatataaaa ttgaaaccta ctatttatga cattgcgaaa
    22321 aaagctaatg tctcaaaatc aactgtctcc agagtattaa ataaccagcc aaatatctct
    22381 gataaagcaa aagcaagtgt acttcaagca atcaaagaat tgaattatga accgagcaaa
    22441 attgcaagag gactgtcagg aaatggtttt gacgcgattc tagcattatc tcatcgtact
    22501 tctcatacga cctatggcaa tccttttttt tcagatgtta ttcaatccat ttctgccgtt
    22561 tctgaacaaa ataacttcga cttgattcta caaacggcaa aaacaccaga agaagagcta
    22621 gaaaaatgcg cgaaaaagat ccaagaaaaa atgatccgag gaattattat tttaagctct
    22681 acgatggatg aaacacttct ttttgaattg gatcaatata atattcctat tgtggtaatc
    22741 ggaaaagtcg gaaacgacta cacgaacatc tactcagtgg acacggataa tttccaggat
    22801 agttacgatt tggtggaaac actgatcaag aatggacacc gtcatatcgg ctgtctccat
    22861 gctccgacag aagtttatgt atccaatgat cgtctcaatg gttaccgtca atgcttattt
    22921 gatcaccacc tagatatccg taatgaatgg attatagacg gcggctttcg cttagaagat
    22981 agcatggcag caatgaatga tttaatgaag ctacgagaaa gaccaactgc gattttttct
    23041 accgatgcat taaaatcatt gagtatttac aaagtggcag aacaatctgg aaagcagata
    23101 cctgatgatc tttcgatcgc aggattcagt gatcccttat tgtcctccct attttcgcca
    23161 cagttgactc ggatagacat tccgacaaat cagttaggaa tcaaagccac agaactttta
    23221 ttccaactaa tccaccacca ataccctgaa gaaaaaagaa tcattctttc aaccgagatt
    23281 actctcacag attcgatcca acaacaagaa atgtagaaca gaattataaa agaacagaaa
    23341 cactgaggat aagtcagtta ctctgttctt ttttcaatca ctcatccctc gttaccccct
    23401 cccggtattt tagcgaattt ttcttatttt ccatctgttt tagatagctg tggataacta
    23461 taatgtttgt gttaaactat tttttccaca gggaagaaaa aactattttt aaccacgttg
    23521 tgtataagtt tttatagttc gtttttttaa acacatttac ccttatttca atccatgtta
    23581 tttggggata cttatctcta aaacataaat agaataacag acatgtctac tggttttgtt
    23641 ttattcgagg aattattcaa taatagctag caaaatatta aaaataaccg ctctatcttc
    23701 ccatcaattt attttgcatt caaaaaatga tctatcttta cttttgttga ttgacttctc
    23761 ttggaagatg atcgttccga taccttaatt ggatacgaac cgatgttgtt aagatccttt
    23821 cccacttttc ttctagttag acaaattatt ttagacaaac caaaaaagag gttgtgacaa
    23881 taatttgtca caacctcttt tttaaaatat ctattttgtt tgattgttca ttgcgtcttc
    23941 tgcagccatt tgcgcttcaa tatctgaaat actgtagaca ttttcgcttg ctacaccgac
    24001 ttcttttggt tccatgtcac gataacgttg cataccagta ccagctggga tgatcttacc
    24061 tataataaca ttttctttca aaccaagaag aggatctttc ttaccgcgga tcgcagcatc
    24121 cgtcaagaca cgagttgttt cttggaatga tgcagcagat aagaaactat ttgtttctaa
    24181 tgatgctttt gtgataccta gtaagacagg tcgagaagtt gcaggaacgc ctcctgagac
    24241 tagcgtattg tagttttgat ctttaaagtc tgcgatgtct aataatgtac ctggtaagat
    24301 atctgtatca cctggatcca ttacacgaac tttacgcagc atttgacgaa ccattacttc
    24361 gatatgcttg tcgccgattt ctaccccttg catacggtaa acacgttgta cttcacgaag
    24421 caagtagttt tcaacagata atacatcgcg aacttgcaac aattgttttg gatcgattga
    24481 tccttctgtc aatggtgcac cacggtggat catatcgcct tctgctactt tcatacgtgc
    24541 tgtataagga acagtatacg tacgtgtatc tgtttttcct ttgattgtta cttctttggt
    24601 acgtgttgca ggatcttctg agatatcgat gacatcccct gttacttctg tgatcactgc
    24661 ttgccctttc ggattacgtg cttcaaagat ttcttggaca cgaggaagac cttgagtgat
    24721 atcgtctccg gcaaccccac ctgtatggaa tgtacgcatc gtcaactgag taccaggttc
    24781 accgattgat tgagcggcga tcgtaccaac tgcttctccg acttcaacgt cagagccagt
    24841 tgcaaggttg cgtccgtaac aatgtttgca gacaccatgt tttgtattac atgtgaagac
    24901 agaacggatt gtcactgttt cgacacctgc atcaacgatt tgttttgcga tgtcttcgga
    24961 aatcaattgg tttcctgcaa tgatcaattc atttgtttca ggatgacgta cttctttacg
    25021 ggtgtaacgt cccactaaac gatcttctaa tgattcgatg atttcatttc cctcgcggat
    25081 ggcttggatt tcaagacctc ggtcagtacc gcaatcttct tcacggatga ttacatcttg
    25141 ggccacgtca accagacgac gagtcaagta acctgaatcg gcagtcttca aggctgtatc
    25201 ggtcatccct ttacgagccc cgtgagttga gataaacatt tccaagacgg atagtccttc
    25261 acggaagttc gaaatgattg gcaattccat gattcgacca ttaggagcgg ccatcaaacc
    25321 acgcattccg gcaagctgtg taaagttgga aatgttacca cgagctccag aatcagacat
    25381 catgaagatt ggatttttcg cttccaaacc ttccatcagt ttttgttgga tgctgtcttt
    25441 tgcagcattc caaacagcaa tgacacgttc ataacgttcg tcatctgtga tcagaccacg
    25501 acggaattgt tttgtaatag tttcaacttg tttatgggca ttttcaatga tttcttgttt
    25561 ttcattcagt acactgatgt cagcgatccc tactgtgata ccggcatgtg tcgaatgttt
    25621 gtaccctaag tctttcatac ggtcaagcat cttagaagtt tctgttactt tgaatcgttt
    25681 gaagacttct gcgatgatgt ttcctaagtt tttcttcttg aacggtaata ctaattcttg
    25741 ctctttgata tgtgcaggaa tatcagtacc agcttcaaca aagtatttgt ccggtgtttg
    25801 gacggtcaag ttgaagtctg ttggttcatt cagataaggg aattcaggag gcatgatctc
    25861 gttgaagatg atcttaccaa ctgttgtgat catcgtacgt tctttttgcc attcagtaaa
    25921 tggtttttca cctaatgagt tagggttcac accgatacgt gaatgtaaat gaacatatcc
    25981 gttgcgccat gcgataacgg cttcgttttg atctctgaag accattcctt cgccttcgcg
    26041 tccttcttct tccattgtca agtagtagtt acctagaacc atatcttgag aaggagtaac
    26101 aactggttta ccatctttag ggttcaagat gttttgagca gctaacatca acatacgtgc
    26161 ttctgcttgt gcttcttcat tcaatggaac gtggacagcc atttggtctc catcgaaatc
    26221 ggcattataa gcttcacata caagtggatg caaacggatt gcgcgtcctt gtactaaaac
    26281 tggttcgaat gcttggatac caagtcggtg aagcgtaggt gcccggttca gcaatactgg
    26341 gtgttccttg attacgtctt ctaaaacatc ccatacttcg tcttcttgac gttcgatttt
    26401 acgtttcgca tttttgatgt ttgaagctag ttcgcgttga acaagctcac gcatcacgaa
    26461 tggtttgaat aattcaatgg ccatttcttt tggaagtcca cattggtaca tcttcaagaa
    26521 aggtccaaca acgataacgg aacgacctga gtagtcgaca cgtttaccta gcaagttttg
    26581 acggaaacga ccttgtttcc ctttcaacat atgagaaaga gatttcaatg ggcggttacc
    26641 tggtccagta actggacgtc cgcgacgacc gttatcgatc aatgcatcta ctgcttcttg
    26701 cagcatacgt ttttcatttt gaacgatgat tccaggtgca ttcaagtcta acaaacgttt
    26761 caaacggtta ttacggttga ttacacgacg gtaaagatca ttcaagtcac ttgttgcaaa
    26821 acgtccacct tctaattgaa ccattgggcg taaatctggt ggaataacag ggataacgtc
    26881 cataaccatc cagcttggtt gatttcctga agcgcggaat gcttctaaaa tatccaaacg
    26941 gcggattgca cgtgtacgtt tttgtccttg tgcagatttc aattcttctt tcaattcggc
    27001 tgcttctgca tctagatcaa cattatcaag taattgttta atcgcttctg cacccatagc
    27061 agcttggaat tcttgtccat attgttcacg tttttcgcga tattcgcgtt ctgtcaacaa
    27121 ttgttttttc tctaaattcg tatttcctgg ttcgatgact acatatgaag caaaataaat
    27181 gatttcttcc aaagcgcgtg ggctcatgtc taatacaaga cccatacgtg aaggaatccc
    27241 tttaaagtac cagatatgtg aaacaggagc tgctaattca atatgtccca tgcgttcacg
    27301 gcgaactttc gaacgagtaa cttccactcc gcatcgatca caaacgattc ctttataacg
    27361 gatacgtttg tattttccgc aggcacattc ccagtctttt gtaggaccaa aaatccgctc
    27421 acagaacaac ccttcacgtt caggttttaa ggtacgatag ttaatcgtct caggtttttt
    27481 tacttcaccg taagaccagc ttctgatttt ttcgggagaa gccaaaccta tttgcatact
    27541 ttcgaattta tttacatcga tcaaaagggg ttccctccat ttcatttaat ggcaaaccgc
    27601 aagacggctg atcttcgacc gccttgcagc tcatagaccg gattagtcta aattatcttc
    27661 tgcggtttca aagttttgga tgacatcgtc tttttcatct tccacttgtt cagcagcttt
    27721 cttttctaat tctttggcag tttgctgttc ggcaaatttt gtcaaagcat caacagtgat
    27781 caaatcgtcg tcttcatcgt ccatgtcgcg aagttcgatc tctgtttctt cgatatccaa
    27841 gacacgcatg tccagaccta gtgattgcaa ttctttcacc aatacgcgga atgattccgg
    27901 tacacctggt tttggaattg gttcaccctt gacgattgct tcgtatgttt tcacacgtcc
    27961 gactacgtca tctgatttgt atgtcaagat ttcttgtaaa gtataagccg caccgtaagc
    28021 ttccagtgcc catacttcca tttcaccaaa acgttgtcca ccaaattgag ctttacctcc
    28081 caatggttgt tgtgtaacca atgagtaagg tccgattgaa cgtgcatgca atttgtcatc
    28141 gaccatgtga gcaagtttga tcatgtacat cacaccgaca gacacacgtc catcaaatgg
    28201 ttcacctgta cgtccgtcgt acagaacagt tttcgcatcg ctggccatac cggcttcacg
    28261 aactgtttcc catacgtctt catcacttgc accatcaaat actggcgttg ctacgtggat
    28321 acctaactga cgtgcagcca tacccaagtg taattccaat acttgcccga tgttcatacg
    28381 tgaaggcacc cctaaaggat tcagcatgat atcgattggt gttccatcag gtaggaatgg
    28441 catatcttct tccggcataa tacgggatac aaccccttta tttccgtgac gaccggccat
    28501 tttatcccct tcatggattt tacgtttttg aacgatataa acacgaacta acatgtttac
    28561 gcctggtgac aattcatctc cagcttcacg agtaaagatt ttcacatcgt gaacgatacc
    28621 gccgccgcca tgtggtacac gtaatgatgt atcgcggact tcacgagctt tttcgccaaa
    28681 gatcgcatgc aatagacgtt cttctgcaga taattctgtt accccttttg gcgtgacttt
    28741 ccctacaagc aaatcgccat cttggacttc tgcaccaata cggatgatac ccatttcgtc
    28801 taaatctttt aatgcgtctt ccccgacatt agggatttcg cgtgtgattt cttcaggtcc
    28861 taattttgta tcgcgggctt ctgattcata ttcttcaata tggatagaag tgtatacgtc
    28921 atcttttacc aaacgacggc tcatgatgat cgcatcttca tagttgtacc cttcccaagt
    28981 catgaaacca actaaaacgt tttggcctag agccatttcg ccttgttcca tagatggtcc
    29041 gtctgccaga gtatcccctt tttcgacttt ctcacccaaa tgaacgattg gacgttggtt
    29101 gtagcttgtt ccggagtttg aacgacggaa ttttgttaca tcgtatttat ctaatgcacc
    29161 gttgtcgcgg cgtacgcgga tttcagaagc atcaacgtat tcaacgacac cgtcatgttt
    29221 acataacaaa gcagctcctg agtcatgcgc tgatttgtat tccataccag taccaaccca
    29281 tggtgcttga ggattgatca acggcactgc ttgacgctgc atgttggcac ccatcaaggc
    29341 acggttggag tcatcgtttt ccaagaaagg aatacatgct gtcgcaacgg caactacttg
    29401 tttaggtgat acgtccatgt agtctacttt atcaatagat acttctaagt tttcactttg
    29461 agcacgagcc ataacaacgt cttgagcaaa tgttccatct tcattcaatg ggctgtttgc
    29521 ttgggctacg atgtaatggt cttcgatatc agctgtcaag tagtcgattt ggtcagtaac
    29581 acgacctgtt tcacggtcta cacgacgata aggtgtttcg ataaatccga atttatttac
    29641 tttcgcataa ctggacaaac tattgatcaa cccgatgttt gggccttcag gagtttcgat
    29701 cggacacata cggccatagt gggagtagtg aacgtcacga acttcataac cggcgcggtc
    29761 acgagtcaat ccgccaggtc ctaaggctga aagacgacgt ttgtgtgtca attcgccaag
    29821 agggtttgtt tgatccatga actgtgacaa ctgagaagaa ccaaagaatt ctttgatact
    29881 tgccacaact ggacggatat tgatcaattg ttgaggtgtc aatgtttcag tatcttggat
    29941 cgacattctt tcacgaacca cacgttccat acgtgctagc ccgatacgga attggttttg
    30001 taaaagttca ccgactgaac ggatacgacg atttcctaag tggtcgatat catcgacatt
    30061 gccgatacct tccatcaagt tcaagaaata gctcattgaa gcaatgatgt cggctggacg
    30121 aaccgttttt acagctgctt ctggatagcc gtttccgatc acgtttactt cacgttcagg
    30181 gtctcttggt gaaaatactt tgatgacttg gacagtcatt ggatctgtga ccacaccgtc
    30241 ttctgatgga tagtaagtca ctgaatttag gccattatca atgaaaggag ctagtgtttc
    30301 catgacttgg tgagtcagaa ctgttccttt ttcaacgatg atctcgcctg tttctggatc
    30361 aactaatgtt tcagctaatg tcagattcaa tagacgtgtt ttcaaatcta attttttgtt
    30421 tactttgtaa cgaccaacgt ttgctaagtc gtaacgtttt ggatcgaaga aacgagcatt
    30481 cagcaagtta cgtgagctgt ctgctgtttt tggttcgcct ggacgcaaac gttcgtaaac
    30541 gtctttcaaa ccttcttctg ttcttgagtc gcttgcgttt ttatgcaagt ctttttcgac
    30601 tgtattgcgt aatgtttcgc tgtcgccgaa gatttcaaag atcgtatcat cggaaccaaa
    30661 tcctaacgca cgaactaata cggttaaagg aatcttacgt gtacggtcga tacgtacata
    30721 agagatgtct tttgcatcag tttccatttc tagccaagct ccacggttag ggataacagt
    30781 agaaccgaat ccttcttttc cgtttttgtc gacttttcca tggaaataga cacctggtga
    30841 acggactaac tgagaaacga ttactcgctc tgccccgttg atgatgaatg ttccttgttc
    30901 tgtcattaat gggaaatcac cgaagaatac ttcttgtgct ttgatttcgc cagtctcgcg
    30961 gtttgtcaaa cgcaatgtta catgtaatgg tgcagagtag tttgcatcat gcgcacgagc
    31021 ttctgctact gtatactttg gttctttcaa ttcataatct acaaattcca aagatagatt
    31081 gccattgaag tcatcgattg gcaaaatgtc ttcaaacatt tctctaagtc cctcatctaa
    31141 gaaccattgg taagagtctg tttgaatttc gatcaaattt ggtaattcca acacttcact
    31201 gattcttgcg aaacttctac gttcgcgatg ttttccgtat tttactacgt gtccagccaa
    31261 gctcttcacc cctctaaaat gtcattcaaa aagactaccc gatcaattgt tacaataata
    31321 tttcaaatat tatttttagg taacaacaga cattttttgc ggcttttagg caaaaaaaaa
    31381 ccaaaaatag aacattctct tgaaacctta cttcgtgagc ttctactttt gtttccttat
    31441 attaaagccg aaacggacaa tatttcgttt ctgccttttc ttcatcagat agtttttgca
    31501 acttcatata ctacacttat tttctataaa agtcaacact tcctggcgtt tcttctgctt
    31561 tttcgcaaca ttttcttaat gttttcaatt ttatgttttc aattatctga caattcttaa
    31621 ttgacagacc tttttatctc ggttatactt tagaaaattc attttgtgga ggaacttcta
    31681 tgcgaaccaa catcaaccaa atcgcaccaa cacaactgaa taacttttac tttagctact
    31741 ttagataggt ttgtattcat catgatggat acaaacgtta aacgacagtt tgtatctatg
    31801 atggctaaag aactttgtat tgcagcaaaa agccacgtag acaaaatgct aagtggctga
    31861 gcagtgaaaa tacaaagtaa aagtatcaag cattctactt tttcaattta ttttccgact
    31921 tttcaatcca tttggacatt ttgtctgaat ggattttttg tttcccaaaa ttaaggagga
    31981 aaaagaaatg aaaaaaaata taatcatcgg attaggcgct gccctgctac tagctttagg
    32041 aggttgcgca caaaaagaca attccgcaga ggcatcagat ggacaaaaaa caattggcgt
    32101 attgcaactt gtacaacata actcgttaga tgctgcctat aaaggattca aagaaggatt
    32161 agcagaagaa gggtataaag aaggcgataa tctaacaatc gattatcaaa atgcccaaaa
    32221 caatcaagac aacctgaaaa gtatgagtga gaaactcgtc aaagaagatc ctgatttgct
    32281 acttggaatc gccactccag cagcacaatc tttattaaat gaaacaacca ctatcccgat
    32341 gactgtcaca gcggtcacag acttggaaca agcccattta gtcacatcca atgaaaaacc
    32401 tggcggcaat gtgactggca cgacagatat ggtgccaatc gaaaaacaaa tcggcttact
    32461 gttaaatatc gtgccagacg ccaaaacaat cggcatcatg tataacaacg gtgaagcaaa
    32521 ctctaagatc caagctgatt tggcagaaaa agaattgaaa aaagcgggcg tcaaagtcaa
    32581 agtactgaca gcaaattcca caaatgatgt ccaacaagtg acgaaaagtc tagctaaaga
    32641 tgtagatgga atctatattc ctactgataa cacgttcgca agtgcggcaa gtgttgtcgg
    32701 tgaagtcgca aaagaaacaa aaacacctgt cgttgctggc gcagttgatc aagtcaaaga
    32761 cggcggtcta gcaacagtag gaatcgacta cgaaagcttg ggcaaacaaa cagggatcat
    32821 ggcagcaaaa atcttagacg gcaagaatcc aagtgaactt cctgtcgaaa cagcgaatga
    32881 tttggaactg tatgtcaata aagacatggc caaagcatta ggcatcaatc cagacagcat
    32941 caaagaagct gaataaaaca caggaggatt ctcatgacaa atttgctctc tcttttctta
    33001 tcatctgcat cgcaaggggt cttatgggcc ctacttgcga ttggtgtata cgtcactttc
    33061 cgtatcttgg acattgccga cttgactgct gaaggaagct ttcctttagg tgcagggatc
    33121 gcggcagtaa gtattacaaa tggctatcat ccattgacag cttgtcttct aggcttttta
    33181 gggggtgcgg cagcagggct tgtttccgga ctgcttcaca caaaattgaa aattcccgcc
    33241 cttcttgcag ggatcatcac catgactggt ttatattctg tcacatcccg tgtgatgggt
    33301 gcgccgaaca tctctttatt gggtcagcca acgatctttc attgggtcga gtcattcggc
    33361 ataacaaaag acaacgctgt actgatcgtc ggtctgcttt ttgctttgat cgttgtatgt
    33421 ttacttgttt tgttcttacg tacagaaaca ggacttgcta ttcgtgcaac tggcgataat
    33481 ctggcaatga gcgaagcaaa cgggatcaac acagataata tgaagatcat cggttatatg
    33541 atttctaatg gatgcattgc tctttctggc tccttacttg cgcaaaacaa cggctttgct
    33601 gatttaaatt ctggtattgg aacgatcgta attggacttg cttctattat catcgcagaa
    33661 gttttgttac gtaaccagcc actatttctc cgtctcttga ctgtcgtttt aggtgcggtc
    33721 atttatcgtt ttattttagc gttagtcttc gaactgaatg ttgaaccaag tgattcgaaa
    33781 cttgcttctg ctttcgtgct agtgatctgt ctttctttcc cgcagatcca gcaaaaattc
    33841 catttacctc aaaaaaagaa aggcggtagt gtcaaatgaa tgacgtctta acattggaaa
    33901 atctccatca tacctttgaa aaaggaacga tcaacgaaaa tcatgtcctg aaaggcatta
    33961 atctttcctt agatcccggc gacttcgtga cgatcatcgg tggaaatggg gctggtaaat
    34021 ctactttgct gaacagcatc gccggtacat tttctgtcga tcagggtcaa attttattga
    34081 atggaaaaaa tatcacgaaa aaatctgttg tcgaacgttc gaaatccatc agtcgcgtat
    34141 tccaagatcc taaactaggg acagccgttc gtttaacggt agaagaaaat cttgctcttg
    34201 ctatgaaacg aggaaaaaaa cgcggcttct tccgtggtgt caaaccacaa gaccgaacat
    34261 ttttcaaaga ccagctggtt cggctaaatt tggggttgga aaatcgttta tcctcagaaa
    34321 tcggtttgct gtctggagga caacgtcaag ctattacgtt attgatggcc acattacagc
    34381 gtccagaact gatcttatta gatgaacaca ctgcagcgct tgaccctgct acttccaaaa
    34441 cggtgatgga attgacagaa aagctgatca ctgagcagaa tctcactgct tttatggtca
    34501 ctcatgacat ggaagacgcg atccgctatg ggagtcgact gatcatgctg catcaaggaa
    34561 ggatcgtcgt agatatccag caagaggaaa aacagcagct cacagtgccc caattattag
    34621 cgttgttcaa acaaaacagc ggcacggcat taaaagacga tcaagtactt ttatcttcat
    34681 aatcagaaag aggatgggac aaaaaatgaa cgcccaccct cttctttttt ctctttcctt
    34741 ctttcgtttt tgctttatgt atcaaccgtt gtttcttcga aaactatttt gttctctcct
    34801 tcttcctgat agaatggatg ggaaaggagc ttgatgccat gacaacgaaa gacaaggtac
    34861 ttcaattatt gaaaaagaca ccagattttc tttccggaga aaagttggca cagcaactgg
    34921 aagtttcccg gacaagtatt tggaaagcca tcaaagaatt ggaaaaggca ggctatcgtt
    34981 ttgagcacgg gcctacaggg tatcgttacc tgccctctga cgtactagat gcaaaagaaa
    35041 tccagcaaga gctacggtcg tttattcctg atttgaaagt acaaacgatg ttgacttctg
    35101 aatctacgat gaaagatgcc aaactggcag cagctcaagg agattctgtt tcgaagttat
    35161 ttgttgctga tacacaagaa caagctcgtg gacgttttgg acggcctttt ttcgctgaac
    35221 ctggacgagg aatctacatg agtctcttgc tccatccaga caaacggttt gatgaattgc
    35281 ctcaatatac gatcatcgca gccagtgcct gtgtgaaagc cattgaatct gtaaccggtc
    35341 gttctgcttc gatcaaatgg gtcaatgata tctacatgga tggaaaaaaa gtctgtggga
    35401 ttttatctga agcagtcagt gatatggaaa gcggcaggat cagtcatatc atcattggga
    35461 tcgggcttaa tttttctgtt cctcaaaatc attttccagc tgatctccaa gaaaaagcca
    35521 tctctgtctt tcccgacgga caagctagtg tcacaaggaa tcagctaatc agcaaaatct
    35581 ggcaagaatt gttcacttat ctttctcacc cagaaaaagc gttggacttc tataaagaaa
    35641 aatcattcgt attgggaaaa accgtgtcgt tcaatcaaaa aggaacgact tatgtcggtc
    35701 ttgcaaaaga aatcggtgat catggcgaac tgatcgtcca aacaaatgaa cagaagatga
    35761 gtttgtcttc tggcgaaatc agtctcacat ctatccgtac gcaataatta tcagatttca
    35821 caaaaggaca cctgatctca tacagtgtcc ttttttctga attctcttca aaagactctc
    35881 cttattctat cagctgacag tttctctatc ctctagaaaa aggtattcta aaaatatcta
    35941 taaagcatgg tacactttct ttaaatgaat caatgcaaaa aacaagagag tcatcagttt
    36001 acacgatcaa aagagatagc aggccgcttc ctttttcaat cttatagata gaccatgggt
    36061 aacgataaaa acaaaaaaga acttgcgact tattttgccg taagttcttt tttagctagt
    36121 gccttaatct cttaatccca acaaacgtct cgcatagatc acctgaccag agtgcatagc
    36181 agcatcatca ataatagaaa cgagacgcat tcctcgtgtg actggtggtg tccagttctc
    36241 gtcaattact tcatctagct cggaaggttc cagccttctt aagtaagaaa tagcaaaatc
    36301 agcagctcct cgcaagtatc ctagcagatc ttctatttga ttagcttgga tctgctttgc
    36361 ttgttccaga ctatgcttcc aatcttgttc gttatctgct atcgtcatcg gaaaccggct
    36421 ttcccagtct tgactatgcc aaattggaga agttcctgct aaatcagcaa tctggaaatc
    36481 taattctcga gcggtatgcc aagccagcca agtcattgat ttgatctgcg gggctctatc
    36541 ttccactgga aaacgattgg cctcctcaat agagacgcct tcaaacaaac gttcaaaccg
    36601 atcgtatgcc cgatccacac tctcgcatag catatctaaa taatccattt attttcctcc
    36661 tttttattta tcctacttac tttttcctaa ttctaggata cctacttata tgttactaca
    36721 cacccttatt ttttacactt taacagtcac catcatcaat agtttggaga ttgaaagaaa
    36781 aagatagtgt cgaactatca attgaacaag aagcaacaat atgaacgttt cattaatatt
    36841 ctttaaataa acaatgattt ttcaaacgaa cagtttaaga ctaggagaaa aagagtataa
    36901 taggaaaatg tttgaaagga aaggaagggg aaaaatgaaa aaaagggcaa gtttactagt
    36961 cctagttgct ttgttgttca atatcttttc gtatggtgtg gccgtttcag cggaaacata
    37021 cattcctaaa gaccctggga atatttataa tgatctaact ggtaactatg gcttgttagg
    37081 aattgctagt caattccacg tatttgctaa aaataagaca actgtgaatg cacatacaga
    37141 cggaaatata gcaacacatg aacttgatgc aaaaaataat tttggtacag agcttcatga
    37201 aggtttattg aaccaagaaa taaattatat acaaaaaact gattctttcg ctaattcttc
    37261 aggcatacct acaggtagca caagaatgaa taaatttgtc gttggcagta acgtggatgt
    37321 agcacttcaa gatggtcatc ctacagtcaa tggatccagg cttgatcact taactccaga
    37381 tgatttttat aaagatcgtg ctgggcatga atatattgat ttcgagcagg aatttgctaa
    37441 attgaatgta gcagcaaatg atcttgcaac gatcactcca gctaaaacat atactgctgc
    37501 agattttcca gacatgaaca atcggacaat cgatttaact gatttatcag ataccgggtt
    37561 tttattggta aatatcgatg cagaagtgtt aacgatgaat acgccacttc aaatcattaa
    37621 tccaaatgat caagtagttg tatttaatgt catcaacagt tcttctgcat tgaacgtaca
    37681 gtcaccaatc aaatacaatg atcgttcaaa tcatgaaaca gaagattttt cggatgcgaa
    37741 tatctcttgg aattttggaa atgagatgac tgatttgaca atcagtgctc cttttcaagg
    37801 aacaatcctt gcgcctaatg ctactatccg cattactcaa aaccaagacg gaacgattat
    37861 aggaacaaat gtgattttag atgcagctac caatcgttgg gatcctaacg aaatatttat
    37921 cagcgacaat ggtacagata cgacagacac aagcgacagt acagatacat ctaccacaag
    37981 tgatagctca gattcatcaa cttcaagcga cagtacagat actacctcta ccacaagtga
    38041 tagcacggat acatcagctt caagcgacag tacagatact acctctacca caagtgatag
    38101 cacggacaca tcagcttcaa gtgacagtac agatactacc tctaccacaa gtgacagcac
    38161 ggacacgtca gcttcaagcg acagtacaga tactacctct accacaagtg atagcacgga
    38221 cacatcagct tcaagtgaca gtacagatac tacctctacc acaagtgata gcacggacac
    38281 gtcagcttca agcgacagta cggatacatc taccacaaca agtgatagca cggacacgtc
    38341 agcttcaagt gacagtacag atactacctc taccacaagt gatagctcag attcatctac
    38401 cactacaagt gatagcacgg acacgtcagc ttcaagtgac agtacagata catcctctac
    38461 cacaagtgat agctcagatt catcaacttc aagcgatagt acagacacgt cgtctaccac
    38521 aagcgataca cgtcatgcaa gtgtctcctt ctcaaaaaca agttcggata acaggacgaa
    38581 ctttaactta actaataaaa caagcaatga caatggaaac aaatcaaaaa gagcacttcc
    38641 taaaactgga agtcaatcga ataattggat aacactcgca ggcgttatat tacttgtaat
    38701 cggtttaaga atcactttta tgagttataa gaaatcgaaa aggtaaaaaa atattcaaga
    38761 taaaaaagag caacgtcgct aacggacgtt gctctttatt tttgcttttt atcataaata
    38821 caagaactac aattcaattt gtcaaaaaac acgtagattt ccttctgaga aatttattta
    38881 cgtatcgaac ctttccctat tcagtctttg cgatccaaaa tcagttgggt aagggaaaag
    38941 agattctctt ttgttcgaag agaggtggca ggtaatttct ttatttcctt caaagccttt
    39001 tctgtgtaag aggccgctaa cttcctagct tcttccacgc ctcctgtatg ttcaacgatt
    39061 ttgcgtacgg cgtcgacatc ttcttctgtc attgccgctt tcttctctaa ataaggtagt
    39121 agctttggtt tgtttttttg caaagagtag atcaacggta aagaatagac cccttgacgc
    39181 atgtcttcta gtacaggttt gccgatttcc tggctctctt ttgtataatc taaaatatca
    39241 tcgataatct gaaaggccat cccgatagaa agtccgatat ctcctgcttt ttttgcgaag
    39301 cgctcgcttg ttccactttc aaaagcgcca acagaacaac ttaattggaa cagttcagct
    39361 gtcttacctg aaatattttc caaatattct tttacagaaa gttcataatt atagcgatca
    39421 tccatctgcc ctaattcgcc attcaatact ttttccatac tgcgagaatt caattggaga
    39481 cttttcaagg aagtggagta atcagacaat agtttaaaac agcagacaaa taaatagtct
    39541 cctgcataga cagaaacgac attaccaaac tgactacgga tattaggctg accacgtcgc
    39601 agatccgctt catcaacgat atcatcatgg atcagtgtgg ccgtatggag catttcgata
    39661 gaagctgcca aagcgatggc tttgtctcga tcttgctctg gtccaaattg tgagaacaat
    39721 agctgatagg caggacgcaa cagctttccg ccggcatgga tcgtttcttt gatggcttgt
    39781 tcgacaggtt tgtttttcag gcggacagtt tcttccatcc gttttagtgt ttctgtcaat
    39841 tctttactta aagcaggata gttttcccac attggatgta gcttcataaa aaaactcctt
    39901 gtgtgacgat tattttttgg taaacattgg gctccatttt tgtttggttc cctgctcata
    39961 atctttttgg aatcgttgaa gtgtctgcac atgtcgtaaa agataattcg ctgcgatccc
    40021 gattgcaatc cctgataaaa tccctaaaac tgaaaggact ggcaagtaca acatgactgt
    40081 ccatgattgt gcaatgaaag aagccatcaa aagctgtccg acattatgca tgaaaccgcc
    40141 ggccgcacta attccgatga tactgacacg ttttggtcct aattgcttga ccaataacat
    40201 tccaaaataa ctaagcaaag caccactcat actataaagg aatgtggaaa tcgttccgcc
    40261 tagcaaagta gtcaggaata gtcgtagcca aagtagtaag aagctgtctt gttttttcat
    40321 ggtaaaaatg gcaacgatcg tgattagatt ggctaagcct agttttgcac caggcgcaaa
    40381 agaaaaagga taagggatca tattttccaa taaaccaatg atcactcctt gtgcaacaag
    40441 tagcgaaata taaatcattt tttgtaattt actcatggtg tgtgaaggtc taataaatca
    40501 gactgtcttc gtcacctcca tctgttgaac ggacttcgat cactaattta tgtggcaaac
    40561 agacgatggt ttcgccattt ttcgttgccc agcctcgacg tacgcagatc tggtcatcgc
    40621 aatctgcttc ttttatccgt actcgatcgc catctatctc gatcaagtta taatctccgt
    40681 gttcatcttt gtaagtatat gtgttgctct cttctccagc catcaaaggg aatgttttga
    40741 tttctgtgcc atccacacgc agtaccgctt ctttttctgc tgttgcggtg ttttgctgaa
    40801 gaccaaaaat catgactggt aaaaaagaaa gaacagtcaa aagcaaaata ataaaaacat
    40861 cccaaggttt gagcagactt ttttcagcaa attctttaag gctcattttt gttctccttt
    40921 ttccagatta actcacccaa ttttagcata aaaacaagag gttgtgacac ctgtgtcttg
    40981 ctttgagtaa taagaacgaa gaatagaaaa cagctttcca tgttttctaa ttctttgggc
    41041 ttattaccga gaagcagcag gtggaacacc gtttataaca agaggttgtg acagaagtgg
    41101 gcaacttcaa gaaataagaa ggaatttacg aaaattactt ttcaagtttt tgtgaaattt
    41161 cggcttattt ttcgaagaag ctacttctgg aacaccgttt ataacaagag gttgtaacag
    41221 aggttgtgac acctgtgtct tgctcatgga ggcttatctc gggcatctta ttcctttaat
    41281 tttacataaa aaggaggctg tgacggtttg tctcagcccc tttctattga aatcttgcat
    41341 tcaaataacg ttctttatct atttatttct tgccgtactg ggaacttggt ctaccgtacc
    41401 accaattacc tagtgtacgt tgtagccatg gaattaccca gcgatccaac ccaatcgcac
    41461 gtccggaacc gttcatcaat gcgaaagcaa caaaaatgaa ccaaatgttt acccagtaaa
    41521 acatgcctga taagcagaac gtgatcacca atacaatcgt tgtcgcactg catagccatg
    41581 tgaataatcc agcaatcaat gctaatgcca aggcaatttc aaagaatacc atgaattttt
    41641 ggaagaacat tgctacatca ttgtttggca tgatcacttg catgatcttt gtgaaccagt
    41701 ctggtgactt ctcaatcaca gccattggtt cttcaccgta tgcataactc aaaccaaaat
    41761 gtgcagcttg agtcgctgct tctccggcac ttgcccctgc tccgctggca gctgtggttg
    41821 catcaccagc agcagaagca ccactggcaa catctgttac attgtattcc catgtccaag
    41881 ggaacacact gtttccagtg aaccatgatc cttcaccaaa ccaagtactt ggtttgaaca
    41941 cttcaccatt cccaatgatt tttttggaag cttcaacgaa ccaaactaaa ccatagaaaa
    42001 tacgaagtgg cacactccat aagacattgc cgtaacgaga agaatgtcct cgagtcacgt
    42061 tgcgatcatc tttgatatgg aaaaactcat gcattaaata ttggaacata taataaccag
    42121 aacgaatatc aaagaagtat ttcaaattca cgatatgttt cattacgata gctaaaaatc
    42181 cgcttaaatg gatcttatca aataggttag ctacccccca tttggcacca atagaaacca
    42241 taaatccttg atagtttcct ttaaatttat gcttttctcc gcctttgatc gaagccacga
    42301 tattcgctgc ggctgtgtgt cctgtttgtt cagcagcttg aacgatctgc ggtgtcggtg
    42361 ttcctgggaa ttcttcataa aagactaaat caccaatgat atagatgttt ttatcttcgt
    42421 agccttttgc ctgcatatat tcattggcga tcaaccgctg tccgcgtgct gattctaatc
    42481 caaagtcagc agcgtcagaa gttcctttga caccagctgt ccaaatcagt gtgtgagtcg
    42541 gaatgttgcg tccgtctttc aatttgatat gatcagcagc cacttctacg attggtgcat
    42601 tcaataataa ttccacgttg tgcttacgca aatagcgttc agcttttgca gcatcatttc
    42661 gatccaacat attcaagatc gtcggcattg cctctacgac catcagtgtg atttcgtttg
    42721 gatctaattt ataatctttg gctaggcgat ttttccagtc gatcagttcc ccaatcattt
    42781 caataccggt aaatccagaa ccacagacaa cgaaagtcag catggcttta cgcagttcat
    42841 catctggttc caatgcagct tttgctactg tcttctcaat atgttcccga atccttacag
    42901 aatcttcaaa tgaccaaagg gtgaatccgt gtgctttgac accaggtgta ccaaagtcat
    42961 tcggctctcc gcccattcca aggatcagct gatcaaaagg ataggaacct aatttggtct
    43021 tgacgatttt tcgttcttta tcgataccga ttacagtgtc tgtgacaagt tgtacattct
    43081 tttgacgagc aaacaaatgc tgcagatcgt attggattgc ttctggttct acacgtccgc
    43141 cagctacttc atgcaactcg gtcatcatag tatggtacga atgacgatcg atcagagtaa
    43201 ttgctacatc ttcttttttc agtttctttg ccaagtattt tgtagctgaa acaccggcat
    43261 atcctgctcc tacaacgaca atattttgct ttgccattga tcatccgctc cttatagact
    43321 atctatataa tgaatatatg ataattcttt tagtttgata cttattacac aatctccata
    43381 tattatatag atgttctgtt cttttgtcta actccttttg gatttttttc aaattttaaa
    43441 ataaagcgtt ttttcacgaa atttgctgaa agaaaacgtt tgaaatatta catgggttat
    43501 ttatcaaaaa tagttgactt ataggctaaa aagattagaa tgtataatga atcacaaaga
    43561 aaaattgtgg actagaaatg gaatggtggg gtattaaaat gaagaaaaaa cgttttgtaa
    43621 ctggatttgc cgtattagca ttttcagctc tagtgttagg cgcttgcggc gcagatgaca
    43681 acggaagctc aaactcttct tctgaatcat ctacagcaca atcttcgact gcaaaaagca
    43741 caacagaatc atctgcaaaa gttgtagctg gcggagattt gcaagacggc acatataaat
    43801 tagaagaaaa aaattattca aatggttacc gtgcagtctt tgaaatggtt gtaaaagacg
    43861 gcaaaatcac tgaatcaaaa tatgataacg tcaatgaaaa cggcgaatca aaaacaaaag
    43921 atgcagaata caacaaaaac atggaagcaa aatctggcac aaacccagaa aaatacattc
    43981 ctgagttgaa tgaccaattc ttaaaagcac aaagcgcaag cggtgtggaa gtcgtaactg
    44041 gagctactca ctcatctgaa agcttccaaa actacgcaca acaattgatc caagctgctc
    44101 aagctggtaa cacagataca atcgaaatcg acaatggtgc tgatttgaaa gacggcactt
    44161 acaagcttga agaaaagaac tactctaacg gctaccgtgt gcaattcgaa atgacagttg
    44221 ctggcggcaa agtaactgaa tcaaactttg attatatcga caaagacggt aaatcaaaac
    44281 aagatgatac agaatataat gaaaatatga aagcaaaaag cggtacagaa ccaaaaactt
    44341 acatccctac tctgaacgac gaattcgtca aagcaatggg cgaagaagat ggctcacctg
    44401 ctaatgtaga agttgttaca ggtgctacac acagctcaca ttcattcatc atgtacgcac
    44461 aacaattagt gaatgcagct gaaaaaggcg acactcaaac aatcgaagta gataatatcg
    44521 taacagaaaa ataatcacat aatcatacta ataaaagttg gagggtggga cgatagccag
    44581 attaggcagt cgtctcacct ttcaaaatga agagaggaaa aagatgcgga aaaaactact
    44641 attttcctta atcacactgg gcatctcttt tatggtgcta actggctgca gcaactcagc
    44701 aaataaaaca accactgaaa gttcagcagc taccaagata ttgaaagatc catattctga
    44761 tgaacagttc ttgttaggga cttacgttcg tatccgtgtg tatgatgaag gcaaagaatc
    44821 agcattgaaa cctgccttcg atcgagtaaa agaacttggt gataaaatca cgatcaatca
    44881 aaaaggttct gagatcgatg aagtgaatga gcaagctggg atcaaaccag taaaagtttc
    44941 tgatgacgtc tacacgttag tcaagcgggc ctacgaatat agccaagatt ctcaaggcgg
    45001 attcgatatg gcgattggtg caatcacaca attatggcga atcggttttg atgacgccag
    45061 aaaaccttcc caagaagaaa tcgatcaggc attgaaatta gtggattatc ataagatcga
    45121 actaaatgac aaagaaaaaa ctgtttatct aaaagaaaaa ggaatgatca tcgatttagg
    45181 tgcaatcgcc aaaggttaca ttacggacga agtagtgaaa gtcttgaaaa aacaaggagt
    45241 gaccactgcc atcgttgact tgggcggtaa tgtctatgtc ttaggccaca gcccaagagg
    45301 cgaaaaccaa gactggacag tcggcatcca agatccgaac atggctcgcg gatctgtttt
    45361 aggttctatt aaagaacgga acaaaacatt agttacctct ggaatttacg agcgttattt
    45421 agaagtagat ggcaaaaaat accatcattt gtttgatcca aaaacgggtt atccgttcga
    45481 caatgatatc gcaagtgtca cgatcataac agataaatca attgatggtg acgggttgtc
    45541 tacagcagtc ttttcaatgg gtgtgaaaaa aggactggaa tacgtcgaaa gcgaattgaa
    45601 taacgggacg gaagcgatct tcgtgacaaa agacgataaa gtatatgtga ccgatccaat
    45661 caaagacacc ttcaaattat cggaggactc ccattacacg atgggtgacc gttctgaact
    45721 aaaataagga gtacgaacca tgacactaaa aatcttttta gaagtagttg aaatacgaac
    45781 aaaggtggct agtgtatttc cttttgcggt aggggtatta ttctctatcg ctttttttca
    45841 tgagatacat tggctaaaca cttttttatt ttttattgga atgctagttt ttgatttagc
    45901 gactaccgct atcaataa
//
LOCUS       DACDKM010000012        43527 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20834_2016
            SAMN12640370-rid9891663.denovo.012, whole genome shotgun sequence.
ACCESSION   DACDKM010000012 DACDKM010000000
VERSION     DACDKM010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640370
            Sequence Read Archive: SRR10040891
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 43527)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 43527)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742835.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742835.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:04:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,012
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 29 of 75
            Pseudo Genes (incomplete)         :: 33 of 75
            Pseudo Genes (internal stop)      :: 19 of 75
            Pseudo Genes (multiple problems)  :: 5 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..43527
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640370-rid9891663.denovo.012"
                     /isolate="E20834_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(167..2548)
                     /locus_tag="IV334_000663"
     CDS             complement(167..2548)
                     /locus_tag="IV334_000663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HAP8942757.1"
                     /translation="MANEQTRSSRRQKQPTPKKSVKKNSGKGSGKSSGTHKKGLFIKI
                     LLGILSFFCILFLAGVGLFWYYAKDAPELTDKKLDATVSSKLYTQDGELFEDLGAEKR
                     EKISANELPKTLEDAIVSVEDRRFYKHIGVDPIRIIGSALSNFTSGGLQGGSTLTQQL
                     IKLSFFSTSAEDQTLKRKAQEAWMAVRLEQKKSKQEILTYYVNKVYMSNGLYGMETAS
                     EMYFGKKLSELSLPQTALLAGMPQAPSAYDPYVYPDQAKKRRDTVLYTMLQNEKISQT
                     EYDQAVNVPVTDGLQELTQSDDNTKIVDNYVKEVINEVQEKTDKNVYTDGLEIYTNLD
                     LDAQKKLYDIVNTDQYVSYPDDEMQVASTLIDTNTGKVKAQIGGRHIAEDVTLGNNLA
                     VNTSRDFGSTMKPVTDYGPAFEYLKYSTGKTITDAPYNYEGTSTPVGNWDNQYMGTIT
                     LRQALYLSRNVPAVKLFNEVGSDKVASFLKNLGIEYSTIHQSNAISSNTEEQDGTKYG
                     ASSLKMAAAYAAFANGGTYYKPQYVNKIVFQDGTEETYEPDGKTAMSPETAYMITDIL
                     KDTITEGTGTNAQIAGLYQAGKTGTSNYTDDEYAKLGISSGVYPDILFAGYTPNYSIS
                     VWTGYNKKMTPVTSESSHVASDVYRELMQYVSANVTNTDWEMPSGLIRVGGELYYKDQ
                     YTARSNAITPSTTIPSSSYVQTPGSSTTETTTQSSSSTSQSESTAESSKESTTAETSE
                     PASSTTVPSSSSEESSTPSSSAPPASSSEPATSGADAANDHTPSSSTSASGNR"
     gene            complement(2538..3185)
                     /gene="recU"
                     /locus_tag="IV334_000664"
     CDS             complement(2538..3185)
                     /gene="recU"
                     /locus_tag="IV334_000664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RecU"
                     /protein_id="HAP8942758.1"
                     /translation="MVLRYPNGQPYYKNASPVEKIQEKRNQITEFGNRGMRFEEAINE
                     SNKYYLSRGLAVIHKKPTPIQIVKVDYPKRSAAVIKEAYFTQASTTDYNGVYRGFYLD
                     FEAKETKNKTSFPFKNFHAHQIEHMKACLEQNGVCFVLLWFSSLKRCFYLNSVHLIAY
                     WDAQKEKGRKSLPLSLIEELGIEIKTGISPRIPYLDAVDFYLSTLSIEGESKNGK"
     gene            3249..3788
                     /locus_tag="IV334_000665"
     CDS             3249..3788
                     /locus_tag="IV334_000665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1273 domain-containing protein"
                     /protein_id="HAP8942759.1"
                     /translation="MDKSKVIYVSGYRSFELGVFKENDPKIQVVKNVLKKELHQLAEE
                     GLEWVLVSGNLGVELWAVQIVAELKEEYPELSLGILYPFSDFGNNWNEGNQEKKQLAE
                     QLADYVEAVSHQPYQSPSQLKNHTRFILEHTVGCLLIYDEEFPGKTKYFFEDAQNYQA
                     DHPYEIRLISMDDLQNFSE"
     gene            3878..4297
                     /gene="gpsB"
                     /locus_tag="IV334_000666"
     CDS             3878..4297
                     /gene="gpsB"
                     /locus_tag="IV334_000666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division regulator GpsB"
                     /protein_id="HAP8942760.1"
                     /translation="MANLVYSPKDILQQEFKTKMRGYDPVEVDEFLDNIIKDYEAYSK
                     ELLALQEENDRLSAKVAQLSKTQGAAQTRVQQTEAPKSAAVTNFDILKRLSNLEREVF
                     GKKLDQQASAVKPAQPNPNNYTNADTSLDDNEKTRQF"
     gene            4319..4686
                     /gene="rnpB"
                     /locus_tag="IV334_000667"
     ncRNA           4319..4686
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="IV334_000667"
                     /product="RNase P RNA component class B"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00011"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00011"
     gene            4895..6061
                     /locus_tag="IV334_000668"
     CDS             4895..6061
                     /locus_tag="IV334_000668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent RNA methyltransferase"
                     /protein_id="HAP8942761.1"
                     /translation="MEKNRKFNLVATAASGLEALVGKELRDLGIECQVENGRARFEGT
                     METIATANLWLRTADRVKIVVGEFDAYTFDELFENVKALPWEDFLPLDAAFPVAGKSI
                     KSKLYSTPDCQAITKKAIVERLRTYYHRPASVPLTETGAHFQLEVALLKDHVMVTLDT
                     TGPSLFKRGYRLEKGGAPLKENMAAALIMLTNWRKDRPFYDPVCGSGTLCIEAALIGH
                     NIAPGFNREFACESWDWFSQEIMENVRSAAEEKADYDIELDITGSDINGRMIEIAKAN
                     AEEIGLGSSITFKQQAVKDFKTDKEYGVIVANPPYGERLGEEETVRKLYKEMGDVFRP
                     LKTWSKYILTSDLAFEEFYGQKATKKRKLYNGALRTDLFQYWGTRPPRKKEKDE"
     gene            6107..7603
                     /locus_tag="IV334_000669"
     CDS             6107..7603
                     /locus_tag="IV334_000669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase M32"
                     /protein_id="HAP8942762.1"
                     /translation="MKEKEFLQEVKEINLLQQALGILDWDTQTGMPEKASAYRSEVDS
                     YLYSIYFSKKIGPKIKEAIRYFSEHPEELSEVGTAVFAKVKEEYDLEHRVPEDLMRKY
                     TAAVSSAHGQWQKARETQQFSDFQHALNQNIELTKQLIPYWRKDEKTNYDVLLNQYEP
                     GMTVEILDKVFDQLKEGILSIRRTLQEKGTEPETDFLNRRMTKKQQKRFVVKVIEQLG
                     YDFSRGRLDDTVHPFMTGINPNDARITTRWNETDFKMAVFGVIHEAGHGMYEQDIDDK
                     YAYTPVYEGTSMGIHESQSLFNEIIIGSSRSFWKKQYPFFQECAEGTFDDIPFDKFYR
                     SLNHTKSSLVRIEADSLTYPLHIIIRYEIEKMIFNEGVDVAELPEIWNQKYEEYLGIR
                     PTNDVEGILQDVHWSGASFGYFPSYALGYMYAAQLLHSLQQEIDVENVLASDDYSPIK
                     NWMSEHIHQYGASRKPNQLIMDATHEPLNPQYLIDYMKDLYFNVYQVK"
     gene            7684..8367
                     /locus_tag="IV334_000670"
     CDS             7684..8367
                     /locus_tag="IV334_000670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HAP8942763.1"
                     /translation="MDKLQVIADYSMDQLAHDQTGHGSDHTKRVVKLAEKILETEPQA
                     DRFVTLAAAYLHDTIDDKVVKNENEAKQQLRVFLRSLPIVEEQISMIFSIIENMSFSK
                     NLSEAAELSLEGKIVQDADRIEALGAIGILRTAYFGGGHGHPIFDPELYPQTFKDKEN
                     YRKGTTVINHFYEKLFLLSDKMNTDYGYEEAKRRETFMREFLEEFFLEWSIDDENFTP
                     DNWIKNNKK"
     gene            9012..10202
                     /locus_tag="IV334_000671"
     CDS             9012..10202
                     /locus_tag="IV334_000671"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HAP8942764.1"
                     /translation="MSSKELTMYLEKELDQLKEKGLYNTIDILESENGACIIVDGKKM
                     INLASNNYLGFANREELKKACIEATETYGVGAGAVRTINGSLKIHQQLEEKIAEFKGT
                     EAAIAFQSGFNCNMGAISAVMTKEDAILSDELNHASIIDGCRLSGAKIIRIKHQDMKD
                     LEKKAKEAIESKKYKKIMYITDGVFSMDGDIARLPEIIPIVEKYGLITYVDDAHGSGV
                     TGKGAGTVKHFGLSDKIDMQMGTLSKAIGVVGGYVAGSKTLIDWLKARSRPFLFSTSL
                     TPGAAAAALASITLMQEHPELVEKVWENANYFKEELKKVGYNIGMSETPITPVILGDE
                     KVTQTFSKKLIEHGIYAKPIVYPTVPLGTGRIRNMPTAEHTREMLDEAVAVYQKIGKE
                     MEII"
     gene            10304..11257
                     /locus_tag="IV334_000672"
     CDS             10304..11257
                     /locus_tag="IV334_000672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine 3-dehydrogenase"
                     /protein_id="HAP8942765.1"
                     /translation="MKKVLVTGCLGQIGSELITRLRQELGSENVIATDIRMPEVIEKN
                     DIFEILDVMDYEKMRSIAEQYQVDTLIHLAALLSAVAEAKPQLAWNLNVTGLVNALEV
                     AREFQLKFFTPSSIGAFGPNTPKDHTPQDTLQRPTTMYGVTKVAGELLCDYYYVKYGV
                     DTRGVRFPGLISYATLPGGGTTDYAVDIYYSAIKEGKYTSFIDQGTSMDMMYMPDAID
                     AIIKLMNADPDRLVHRNAFNISAMSFDPEEIKASIQKIIPDFEMDYDVDPVRQSIAES
                     WPNSLDVSCAQEEWGFSPKYDLDVMTKDMLEKLKEKLVETV"
     gene            11326..12675
                     /locus_tag="IV334_000673"
     CDS             11326..12675
                     /locus_tag="IV334_000673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAP8942766.1"
                     /translation="MEEYQATPVKEVKIENELKRTMGFFTALSTVMGTVIGAGVFFKA
                     ASVAEVTGSASLHMFSWFLGGMISVCAGLTGAELAAAIPETGGMIKYIERIYGNTAAF
                     LLGWAQVVIYFPANVAALSIIFGTQFVNLFGLSQSMIVPVAVTAAVSILLINFLGSKA
                     GGAFQSITLVCKLIPLFVIVIFGLFRQEGVDFQLFPIQAGENLSFFSALGAGLLATMF
                     AYDGWIHVGNISGELKKPAKDLPKAISLGIIGIMIVYLLVNAVFLRTASIDGVAGNSN
                     AASDVAKMIFGGFGGRLVTVGILISVYGTINGYTLTGMRLPYVMAKENNLPFSKLFAK
                     LHDKTKVPVAAGILELVIAIGMMMIGGFDTLTDMLIFVIWIFYTMVFVGVILLRKKEP
                     DLFRPYKVPMYPFIPLVAIIGGTFIVSSTLITQTFLASMGIVLTLAGIPIYLYLKRR"
     gene            complement(12858..13181)
                     /locus_tag="IV334_000674"
     CDS             complement(12858..13181)
                     /locus_tag="IV334_000674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8942767.1"
                     /translation="MLNMYFVFGVPIFLLFLYATIAYVRKRTTIHYLGFILLIISGFM
                     LVFNLQTWQQALLEMDKMTPHALSKVLGYPVYLIWLPIFISGCLVLLNIYRGVRRIVQ
                     LRKSK"
     gene            13310..14473
                     /locus_tag="IV334_000675"
     CDS             13310..14473
                     /locus_tag="IV334_000675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8942768.1"
                     /translation="MASYIEPIRFALIVFPFLALAISSVFFIYEYRKYGTFLVTRAVI
                     LYSFVFYLLCAYFLVILPLPAKETVAQMTGPKMELHLFASWQHFRDNTVLVLSEPSTY
                     LPAMKQSVFLEPAFNVLLLIPFGIYLRYYFRLSFMKTMIASFCLSLFFELTQLSGLYF
                     IYPRPYRLFDVNDLVTNTLGGIIGFVVTPLFTFMLPTRRRMDELSYEKGKTVSLTRRL
                     VAYLIDWTFLSLINLVAAIFFKIAFDSGAVTSHIWWLLLEVGVYFMIVPFITNGWTLG
                     KKIVRIRLVEEDRERISLKALMIRYGYLYFLFYGLSWLGQLLQQIMNSTTNTVLQMSA
                     VFISLGVLLLQGIFILNILLSFLRKKRILFYEKASHTYTVSTIGQTKDDDSRN"
     gene            14543..15892
                     /locus_tag="IV334_000676"
     CDS             14543..15892
                     /locus_tag="IV334_000676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HAP8942769.1"
                     /translation="MNNFDIFRFKPFINEALTAKGFKQPTEVQERLIPLIAKGKNIIG
                     QSQTGTGKTHTFLLPLFEKINAEKEEVQVVITAPSRELAAQIYEAATQIAKFSTPEIR
                     VSNFVGGTDKQRQLNRLHHQQPQVVVGTPGRILDMMNEQALKIHTAFAFVVDEADMTL
                     DMGFLNEVDQIASRLPEKLQFLVFSATIPEKLRPFLRKYMENPIIEEIKPKAVISEDI
                     ENWLISTKGKDENQMIYQLLTIGHPYLAIVFANTKQHVDEIADYLKNQGLKVAKIHGD
                     ITPRERKRVMRQVQNLEYQYVVATDLAARGIDIEGVSHVINAEIPEDLDFFIHRVGRT
                     GRNGLKGTAITLYSPNDEQAIDAIEKLGVSFEPKEIKNGEIVKTYDRNRRAKREKSKD
                     VLDPTLIGLVKKKKKKIKPGYKKKIDWAIAEKNKKERKIERRQQSRTARKNKKNSHK"
     gene            complement(15937..16368)
                     /locus_tag="IV334_000677"
     CDS             complement(15937..16368)
                     /locus_tag="IV334_000677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP8942770.1"
                     /translation="MKKIVGADEWIRAASCFLRYQVFVLEQGIPAELEFDGKDIPQQT
                     YYLFLDQKRPVATLRYEKKTPTCFKPERFCVAKEYRGKGLGGQLLEAAEKRAVSEGCQ
                     TSYVVAEMTAVDFYEKQGYHTCTEPFIEDEVLCIGMKKSLA"
     gene            16756..19398
                     /gene="alaS"
                     /locus_tag="IV334_000678"
     CDS             16756..19398
                     /gene="alaS"
                     /locus_tag="IV334_000678"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HAP8942771.1"
                     /translation="MKQLSSAQVRQMFLDFFQSKGHTIEPSASLIPVNDPTLLWINSG
                     VATLKKYFDGSVVPENPRITNAQKSIRTNDIENVGKTARHHTMFEMLGNFSIGDYFKN
                     EAIHWAWEFLTDPKWMAFDPEKLYVTVYPKDTEAKRIWHEEVGLPLDHIIDVEDNFWD
                     IGAGPCGPDTEIFYDRGEAYNDLAEDDPENYPGGENERYLEIWNLVFSEFNHKPDNTF
                     EPLPHKNIDTGMGLERMVSIVQDASTNFETDLFMPIIHAVEKLSKQVKYGENPVTDIS
                     YKVIADHIRALSFAIGDGALPSNEGRGYVLRRLLRRAVMHGKKLGIEQAFLYRLVPVV
                     GEIMVSYYPEVLQQKEFIEKVIRTEEERFNETINEGLEILNQVIQEVKNENGTTLAGK
                     DIFKLYDTYGFPVELTEEVAEDEGLKVDHAGFEKEMDAQRERARSARSTEKSMGVQSA
                     LLTDIKVESKFIGYTEQESDSQLLVIVKGESLLNEVAEGEAQMIFDQTPFYAEMGGQV
                     ADQGWIMNEVGEIVAVVTDVQKAPNGQFMHTVEVKAPMSEGQTYHLLIDQSMRNRIIK
                     NHTATHLLHKALKEVLGEHANQAGSLVAPGHLRFDFTHFGQITSDELAEMERIVNEKI
                     WEAIPVETIETDIDTAKNMGAMALFGEKYGHDVRVVNIGGWSIELCGGTHVANTENIG
                     IFKIVSESGIGAGVRRIEAVTSKEAYELLEEEEKQLKQVATIVKSPQLKEVVSKAEQL
                     QQQVRELQKENEQLASKLANQQAGDVFKDPETINGKTIIAAQVKVKDMNQLRQLADQW
                     KQKNISDILVLANEQEGKVSLLAAMTKESNEAGLKAGDLIKAIAPKVGGGGGGRPDMA
                     QAGGKNPAGIPDALKAAKEWVAQQ"
     gene            19598..20194
                     /locus_tag="IV334_000679"
     CDS             19598..20194
                     /locus_tag="IV334_000679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HAP8942772.1"
                     /translation="MNSRNKTFRLVLRAILLAIIIVQAMVPWLGFIPLGFISLTIIHI
                     TVIIAAVVLGPKDGMVIGLFWGIATIVRAYAMPTTPFDTLVFTNPIISVVPRVLVGLV
                     AGLVFHWIYQKNKSITISSAFAGVLGSLVNTVLVLGFMGLFYTGATADAYGVDSALLF
                     KTLAGVAAINGIPEAIGAGIITPLLAKALFAATPLKPE"
     gene            20316..21464
                     /locus_tag="IV334_000680"
     CDS             20316..21464
                     /locus_tag="IV334_000680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HAP8942773.1"
                     /translation="MAKKQEEKKKQIPQQRTKKGVLAGILGLVTNLILFVSKLMIGLF
                     SGSVSIMADAVNSLSDTASSILTLIGFKIAAKPADQEHPYGHERFEYISGLFVSIIIT
                     YVGFQFLESSAEKIIHPENVSLTPIVFFVLIISIFLKFAQGKMYQVIAQSIDSETLRA
                     TATDSYNDVFTTLAVLLSAAIEWVTGWRIDGYIGFALALYILYSGLTMLRNFVYELLG
                     SRPTPAEIEEMEEHLNACDDILGYHDLLVHNYGPNKRFASVHIEVDESMNLNVAHKII
                     DLIEKDFKKSLDVELVCHLDPMPVDNEKYYNILRQLKQILLETGTGLNIHDFRVIHQG
                     KTLQFDVVVPEKFQFVDTELERILRSQIEEKIGDYQLEITFDHNYLLY"
     gene            complement(21502..21915)
                     /locus_tag="IV334_000681"
     CDS             complement(21502..21915)
                     /locus_tag="IV334_000681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI family protein"
                     /protein_id="HAP8942774.1"
                     /translation="MLKVYIDASTKGNPGPSGGGILIVYQGKQEQLSIPLSIGTNHQA
                     EFEVFLKTLEFLKKNDLQNETIFCFSDSKTLVSTVDKNATKNELFLPYLQRIQELLSE
                     FSLLILQWLPENKNKGADNLARQALHKQLKEKKTL"
     gene            21994..22425
                     /locus_tag="IV334_000682"
     CDS             21994..22425
                     /locus_tag="IV334_000682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EbsA family protein"
                     /protein_id="HAP8942775.1"
                     /translation="MKKNIRWQPELAHTIIYWSLTLIILFFSLILSLENTGPYWKSNF
                     VLGIFLVFLFLGLRRSFILKSNSVIIRYAAFWKDREISVGQIRSLTPTNTGVQMMLQD
                     EKAPSVFMMRKKDQVVLFDHFEKNGPKSESIPYPYTKKRKS"
     gene            complement(22524..22757)
                     /locus_tag="IV334_000683"
     CDS             complement(22524..22757)
                     /locus_tag="IV334_000683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock domain-containing protein"
                     /protein_id="HAP8942776.1"
                     /translation="MTTGIVKWFDNKKGYGFISYDDTEEIFVHFTAIEEEGFKTLEEN
                     QVVEFEIIEGNRGTQAAHVKKGNVQTTDTSMHE"
     gene            22940..24610
                     /locus_tag="IV334_000684"
     CDS             22940..24610
                     /locus_tag="IV334_000684"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HAP8942777.1"
                     /translation="MKNDLQISQETELEPIVEVASKLDLKEDDLELYGKYKAKINFSG
                     INRSKDNAEGHLILVTSINPTPAGEGKSTITVGLGDALNKINKKSVIALREPSLGPVM
                     GIKGGAAGGGYAQVLPMEDINLHFTGDMHAITTANNALSALLDNHIHQGNELNIDARR
                     VIWKRVVDLNDRELRKVIVGLGGPIQGVPREDGFDITVASEIMAILCLAADLEDLKAR
                     LARIVVGYTFDRQPVTAGDLKAEGALALLLKDAIKPNLVQTIYGTPAFVHGGPFANIA
                     HGCNSILATKTALRLADYVVTEAGFGADLGGEKFLDIKVPNLKKAPDAVVIVATVRAL
                     KMHGGMKKDELKNENLDALKVGFANLKRHIRNMEQYQLPVIVAINEFVTDTDNELTLL
                     EHLCENQGILAKRASVWADGAEGGVDLAKAVVRLIDRKEANYKPLYRLEETIQEKTET
                     IVKKIYGGNGVVFSKKAQKQIEEFTKNGWDKLPICMAKTQYSFSDDPSLLGAPEDFTI
                     TIREIIPKLGAGFLVALTGDVMTMPGLPKKPAALNMDVTNDGEVLGLF"
     gene            24757..25143
                     /locus_tag="IV334_000685"
     CDS             24757..25143
                     /locus_tag="IV334_000685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8942778.1"
                     /translation="MKTTFIRKTGFYGMGSSIQLRINNEKRSVLSHEQKITLDLEVPF
                     TMQVTFYWLKSPVYTVKEAKPAYVITMNYLILQIYPFLFLATGLGAVFLQNLFFSFFA
                     VIVLIAFFFYIKNRAYLIKEENYGKL"
     gene            25130..25828
                     /locus_tag="IV334_000686"
     CDS             25130..25828
                     /locus_tag="IV334_000686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HAP8942779.1"
                     /translation="MANSDLFLISFNRIEKWMRDEMGNARNMGFTELVRRLAQRKQLM
                     IRKYEDDLLQLAQLRNAIVHDRIAVDFVIAEPNDWTVKRIQLIEQELIQPETVLPRFA
                     KRVTGFEQDLPLLELLKIVAEKRYSQFPLYNKGKFVALITLRNIGFWLAKESQKGPVD
                     LTNKRALDLIIQNGKYTNYHFVPAKTHIYEVEAMFREQGTLEAVLITKDGNPDGNLLG
                     IVRPRDIYKQIEKD"
     gene            25833..26297
                     /locus_tag="IV334_000687"
     CDS             25833..26297
                     /locus_tag="IV334_000687"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HAP8942780.1"
                     /translation="MLIIYWIFSALLVGVDQLVKWWITDNLALGETKNLIPGILSLNH
                     IRNTGAAWSMLEGKMWFFTIVTLIAVVVILTLMIKNREKGNRWFMIGLSLILAGAIGN
                     FIDRIRLGYVVDMFQTDFMSFPIFNVADVTLVCGVICMLIYIILDEKDQRKK"
     gene            26294..27205
                     /locus_tag="IV334_000688"
     CDS             26294..27205
                     /locus_tag="IV334_000688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HAP8942781.1"
                     /translation="MTELKTTIHHESGRIDKVLTNLFTDYSRSQIQLWLKNGAVSVNG
                     QPVKANYKVKQGDEITVLIPEPETLDVVPENIPLTIVYEDEDVAVINKPQGMVVHPSA
                     GHTHGTMVNALMYHIKDLSTINGVIRPGIVHRIDKDTSGLLMVAKNDQAHESLAQQLK
                     DKTSLRKYVALVHGEIPHEKGTINAPIGRSKENRKMQAIREDGKSAVTHFTVLERFEG
                     FTLVELKLETGRTHQIRVHMKYIGYPLAGDPVYGPKKTLKGNGQFLHAQMLGFTHPTT
                     NEKMIFEAPLPEVFEKTLEKLRKNIAF"
     gene            27440..27979
                     /gene="pyrR"
                     /locus_tag="IV334_000689"
     CDS             27440..27979
                     /gene="pyrR"
                     /locus_tag="IV334_000689"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015694870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HAP8942782.1"
                     /translation="MQAKEVVDQVTMKRALTRITYEIIERNHSIQDIVLVGIKTRGIY
                     IASRIAERLKQLEDIDIPVGELDITLYRDDKKENPEEPELHSSDIPVSLEGKEVILID
                     DVLYTGRTIRAAMDAVMDFGRPRKISLAVLVDRGHRELPIRADYVGKNIPTSRAEEIL
                     VEMQELDGQDRIMILKEED"
     gene            27993..29276
                     /locus_tag="IV334_000690"
     CDS             27993..29276
                     /locus_tag="IV334_000690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil permease"
                     /protein_id="HAP8942783.1"
                     /translation="MSEKKGFHNDDVVLDIHDRPTPGHWVGLSLQHLFTMFGATVLVP
                     ILVGIDPGIALVSSGLGTLVYLFTTKGKIPAYLGSSFAFIAAMQLLMKTDGYPAIAQG
                     AVTTGLVYLIVALIIKKIGSAWLDKILPPIVVGPVIMVIGLGLAANAATNAMYNQGTY
                     DFKYIAVALITLGLTIFFNMWLRGFLGLIPILLGIISGYIVALLFGIVDTKPIVDAAW
                     FAVPNFEIPFVQYEPKLYLGAITTMAPIAFVTMTEHIGHLMVLNKLTKRNFFEEPGLH
                     KTLTGDGLAQIVAAFVGGPPVTSYGENIGVLAITRVHSVFVIGGAAVFAVILGFVGKL
                     SALILSIPGPVISGISFILFGVIAASGMKILIENKIDFDKKKNLLIASVILVVGIGGL
                     VFEVGTFTLSAMALATVLGIILNLILPETSRSEEN"
     gene            29289..30215
                     /locus_tag="IV334_000691"
     CDS             29289..30215
                     /locus_tag="IV334_000691"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HAP8942784.1"
                     /translation="MIIKSERISLKHLLTVEALTDQEVMGLIRRGQEFKQGANWTPQK
                     RQYFATNLFFENSTRTHKSFDVAEKKLGLEVIEFEASTSSVQKGETLYDTVLTMSALG
                     VDVAVIRHGDENYYDELIQSKTIQCSIINGGDGSGQHPTQCLLDLMTIYEEFGHFEGL
                     KVAIVGDITHSRVAKSNMQMLKRLGAQVFFSGPREWYDDEFEVYGHYMPLDELLDQVD
                     VMMMLRVQHERHDGKESFSKEGYHQEHGLTVERAKKLQKHAIIMHPAPVNRDVELADS
                     LVEGLQSRIVQQMSNGVFVRMAILEAVLNGKA"
     gene            30234..31514
                     /locus_tag="IV334_000692"
     CDS             30234..31514
                     /locus_tag="IV334_000692"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HAP8942785.1"
                     /translation="MKTLIKNGQINTRKNMTTPAEIWIEDGRIKAIGTGFSEAEFDEV
                     FDAKGQLITPGLVDVHVHLREPGFTYKETIEAGTRSAARGGFTTVCAMPNLNPVPDTA
                     EKLRQVYDIIRKDAVVKVLQYAPITENLRSEKLVDQEALIEEGAFAFTNDGVGVQTAG
                     TMYLAMKEAAKNNKALVAHTEDESLLFGGVMHAGKKAEELGLPGILSVTESSQIARDL
                     LLAEATGVHYHVCHVSTKESVRVIRDAKKAGIHVTAEVSPHHLILIDEDIPEDFGFWK
                     MNPPLRGREDREALIEGLLDGTIDCIATDHAPHGLEEKSQSFMKSPFGIVGSETAFQL
                     IYTHFVETGRFTLEQVINWLAVKPAEIFGLNAGTLTVGAPADVAVFDITQTCTIDKED
                     FLSKGENTPFIGWKVKGETQMTFVNGKLVWQKGE"
     gene            31519..32595
                     /gene="carA"
                     /locus_tag="IV334_000693"
     CDS             31519..32595
                     /gene="carA"
                     /locus_tag="IV334_000693"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010744830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HAP8942786.1"
                     /translation="MERLLILEDGTVFEGKAFGADASVFGEIVFTTSMTGYQETITDQ
                     SFNGQIITFTYPMVGNYGVNRDDYESISPTCKGVVVKEHARVASNWRNQMTLDSFLKQ
                     KGIPGISGIDTRALTRKIREVGTMKASIVDAGDSFEHAFDQLKATVLATNQVQQVSTN
                     KPYPSPGTGRKVIVIDFGLKHSILRELSKRNCNLTVLPYNTDAETILALSPDGVMLTN
                     GPGDPKDVPEAIEMIKKIQGKVPIFGICLGHQLFALANGADTYKMKFGHRGLNHPVRE
                     VATGRIDFTSQNHGYAVDEATIDPEKLIVTHVEVNDGTVEGVKHRDYPAFSVQFHPDA
                     APGPHDALHLFDEFMEMMDAGKEQ"
     gene            32600..35785
                     /gene="carB"
                     /locus_tag="IV334_000694"
     CDS             32600..35785
                     /gene="carB"
                     /locus_tag="IV334_000694"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HAP8942787.1"
                     /translation="MPKRTDIKKIMVIGSGPIIIGQAAEFDYAGTQACLALKEEGYEV
                     VLVNSNPATIMTDKEVADQVYIEPITLEFVSRILRKERPDALLPTLGGQTGLNMAMEL
                     AASGILEELNIELLGTKLSAIDQAEDRDLFKQLMKELNQPIPESEIVNTVEEAVDFAN
                     KIGYPVIVRPAFTLGGTGGGMCENEEELRIVSENGLSLSPATQCLIERSIAGFKEIEY
                     EVMRDSADNAIVVCNMENFDPVGIHTGDSIVFAPSQTLSDHEYQMLRDASLSIIRALK
                     IEGGCNVQLALDPHSFNYYVIEVNPRVSRSSALASKATGYPIAKLAAKIALGLTLDEM
                     KNPVTGTTFAEFEPALDYVVAKIPRWPFDKFENGERLLGTQMKATGEVMAIGRNIEES
                     LLKAVRSLEIGTHHIELPELADITDDDLIQKVVRAQDDRLFYVAEAIRRGYTIEELAD
                     LTKIDLFFLDKLLHIVEIEQQLKQQPLDDKILKTAKQNGFTDKKVAELWEINEEAVRQ
                     MRKENDLIPVYKMVDTCAAEFESKTPYFYSSYEYENESSRSEKPSILVLGSGPIRIGQ
                     GVEFDYATVHSVKAIQQAGYEAIIMNSNPETVSTDFSISDKLYFEPLTFEDVMNVIEL
                     EQPIGVIVQFGGQTAINLAEPLTAAGVKILGTTIEDLDRAENRDLFEQALKELDIPQP
                     LGDTATSKEEAVVIASRIGYPVLVRPSYVLGGRAMEIVENQRDLEDYMEHAVKASPEH
                     PVLVDRYLLGGECEVDAICDGETVLIPGIMEHIERAGVHSGDSMAVYPPQTLSESIKK
                     TIEDYTIRLALGLNCVGMMNIQFVIHENEVYVIEVNPRASRTVPFLSKVTGIPMAQIA
                     TKAILGEKLKTLGYENGLYPETKEVHVKAPVFSFTKLQKVDTYLGPEMKSTGEVMGSD
                     RQLDKALYKAFEASGLHLPEYGAVLFTIADETKEEALELAKRFTEIGYSLIGTKHTAA
                     YFEKNGLVVETVAKISEEAAEKNVVDLIRDGKTQVVVNTIDKDRGNASKDGFIIRREA
                     VEHGIPLFTSLDTADAIVRVMESRAFSTNPI"
     gene            35839..36615
                     /locus_tag="IV334_000695"
     CDS             35839..36615
                     /locus_tag="IV334_000695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase electron transfer
                     subunit"
                     /protein_id="HAP8942788.1"
                     /translation="MKQEMMEIVQQKQLAPKIFEMTLKGDLVKEMQQPGQFLHILVPR
                     ADLLLRRPISLNHIDHETNTCRIIYRVEGEGTRVFSTLSAGDHLDVMGPLGNGFDLSN
                     LHTGDEVFIIGGGIGVPPLYELSKQLVEKGIKPTHFLGFATHEVVYYEKEFNRLGETR
                     IATDDGTYGMHGNVGNLLLGTKTEPTAVFACGSNGLLKTVEQLFSSHQNVQLSLESRM
                     ACGIGACYACVCHTPEDETGTKSVKVCDEGPIFKIGEVII"
     gene            36612..37544
                     /locus_tag="IV334_000696"
     CDS             36612..37544
                     /locus_tag="IV334_000696"
                     /EC_number="1.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HAP8942789.1"
                     /translation="MNENPLAVKLPGLELKNPIMPASGCFGFGKEYGKYYDLNQLGSI
                     MVKATTPNARFGNPTPRVAETPSGMLNAIGLQNPGLDVVMHKLLPELEEYNELPIIAN
                     VAGACEEDYVEVCSKIGEAPNVKAIELNISCPNVKHGGIAFGTDSDVAFQLTQAVKKV
                     SSVPVYVKLSPNVTDIVPIAQAIEAGGADGFTMINTLLGMRIDLKTRKPILANQTGGL
                     SGPAIKPVAIRLIHQVASISSLPIIGMGGVQTVDDVLEMFMAGASAVAIGTANFTDPY
                     ICPKLIKELPVRMSELGIESLERLIKEVREERER"
     gene            37541..38257
                     /gene="pyrF"
                     /locus_tag="IV334_000697"
     CDS             37541..38257
                     /gene="pyrF"
                     /locus_tag="IV334_000697"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HAP8942790.1"
                     /translation="MNQLDQRPIIALDFSTRQEVEDFLRFFPKEEKLFVKIGMELFYQ
                     EGPEIVRYLKEAGHNVFLDLKLHDIPNTVEKAMRGLAKLGVDLTCVHAAGGIRMMEAA
                     MRGLEEGTPEGGKRPLLLAITQLTSTSEEEMHADQLIEVSLEKSVIHYASCAKKAGLD
                     GVVSSAWEVEAIKEMAGDEFVCLTPGIRPEGTVAGDQTRVVTPSQAKKIGSTFIVIGR
                     PITQSTNPYKAYQTIQTEWS"
     gene            38264..38953
                     /locus_tag="IV334_000698"
     CDS             38264..38953
                     /locus_tag="IV334_000698"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HAP8942791.1"
                     /translation="MNVEQSIAKDLLEIEAVFLNPSNPFTWASGIKSPIYCDNRITMS
                     YPKVRKEIAKGLASKIKEAYPEVQVIAGTATAGIPHAAWVAEILDLPMVYIRSKAKDH
                     GKGNQIEGRIFEGQKMVVIEDLISTGGSVLEAAEAAKREGADILGVAAIFTYELPKGK
                     ANFEKAEIPLMTLTNYSVLIEAALEDRYIDEQELTLLKEWKKDPENWQIEARLMKIEK
                     SDLCLQNHQLE"
     gene            38926..39195
                     /locus_tag="IV334_000699"
     CDS             38926..39195
                     /locus_tag="IV334_000699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8942792.1"
                     /translation="MSPESSIGIAKIQKLIARSIFSSGMKRNFYRDQFLIFLKFIFLR
                     LVSRKKRIISSLFSIIYRFSLFFGKGAVSKMVKSGNVANISYLMI"
     gene            39233..39874
                     /locus_tag="IV334_000700"
     CDS             39233..39874
                     /locus_tag="IV334_000700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HAP8942793.1"
                     /translation="MGLNEFILGLNNLETITRAPGFFKYTEHTVAAHSYRVASIAQVL
                     GDIEEANGQPIDWKLLYEKSLNHDYTERFIGDIKTPVKYASKELRGMLQTVEEKMTDE
                     FINQEIPAEFQEVYRRRLFEGKDETVEGELLAISDKVDLLYESFDEIIKNNPEKVYKE
                     MFIEAVTTIKSFEHRPSVQYFFKEIFPELLNQEFYGKEAFLETVSTIVKKEEK"
     gene            39905..40609
                     /locus_tag="IV334_000701"
     CDS             39905..40609
                     /locus_tag="IV334_000701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase family protein"
                     /protein_id="HAP8942794.1"
                     /translation="MKKNMDVEWGYSGDKGPENWASLCDWFARGAEFPLQSPIHLTKG
                     EETKIEGDTLSFHYQKQRFTDKEFKNTIHLVPFDQLSYVEFKKERYYLTDIHFHLPSE
                     HIINGKQEDIEFHFVHMNSKKENLVVGVMYQLMVQEGWLYNQENGESWNLEKHEHWVN
                     PDVFFPEQKSHFHYIGSLTTPPTSGPIQWFVMDHVGKLNQEFLLPFDGQYARPNNRPI
                     QPLNGREIYYFEEFEQ"
     gene            40606..41508
                     /locus_tag="IV334_000702"
     CDS             40606..41508
                     /locus_tag="IV334_000702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAP8942795.1"
                     /translation="MNIQQMKYVVAVANNGSFREAAKKMFVSQPSLSAGIKELETELG
                     ITLFTRTNRGAYLTEEGQDFLNRAEKILVQLDTLETHYLTSEKRDNFSIAAQHYDFLG
                     PLTAQLLEEFKDEIKQFRIMETTTARVIEEVKEQHSEIGIIYMNEHNQAGIRRYLQQG
                     ELSGRSIGKFKTHIFLRQGHPLAEHAEITKEELLSYPQVRFTQDGNNFPYFYEDLIEI
                     PDQETVIYTSDRGTLMNIVLETDAYASGSGIVIGGIREHLRLIPLAEGELNEFYIIYS
                     AKRPLSEIAQRFIKELTRLLDQQS"
     gene            complement(41666..43372)
                     /locus_tag="IV334_000703"
     CDS             complement(41666..43372)
                     /locus_tag="IV334_000703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin/fibrinogen-binding protein"
                     /protein_id="HAP8942796.1"
                     /translation="MSFDGVFTHAMVNELRETLLSGRISKIHQPYENEIVLVIRSRGK
                     NHRLLLSAHPSYARVQITQIDYQNPDNPPNFVMMLRKYLDGAILEDIEQIENDRVIHF
                     HFAKRNELGDLQNIILIVELMGRHSTIVLVNRETGKILDAIKHIGSSQNTYRSLLPGV
                     EYVAPPKQEVLNPFSSEKEKIFQRLSQTELDPKAIQRQFQGIGFDIAQELTKRLLERP
                     NEKMVVWDEFFSAISHQPIPTFYETENKDFFTPIAYQVLSEQASAVTAYPTLSQLLDS
                     YYHEKAEKDRAKQQGGELIRKIENELKRNKNKLKKREQTLKESENAENYRRNGELLTT
                     FLTQVPRGAKEVVLPNYYEEDRPIKIALDPALTPNQNAQKYFHRYQKLKNAVKLIGEQ
                     IQEAKDEIQYLESVLSQLEIAGPMDIEAIKEELTSEGYLKKKNLKKQKRKKPSQPDQY
                     FSSDGTLILVGKNNLQNDQLTMKTAKKTDYWLHAKNIPGSHVIIKSDKPSDETITEAA
                     ELAAYFSKYRYSAQVPVDLVQVKHIRKPNGAKPGYVIYENQKTIIVTPEEEKITAMKQ
                     NR"
BASE COUNT        13928 a         7684 c         9466 g        12449 t
ORIGIN      
        1 aaatggattt tctaattttt aagctaaatg ttagatcaca catgtatatg ctggatagtc
       61 ttataaaaaa tcatgtaata cagcttggta catgattttt atttgtagct gttttttaca
      121 tagacacaaa aaagaaacca cgcttgtaga cgtggtttct tttttactat ctgtttccag
      181 aagctgaggt gctgctggat ggtgtatggt catttgcagc atccgctccg ctagttgctg
      241 gctcactgga agaagcagga ggagcagatg aagacggtgt cgagctttct tcggatgaag
      301 aagaaggaac cgttgttgaa gacgctggct cgcttgtttc tgctgtcgtg ctttcttttg
      361 aggactcagc agttgattcg ctttgactag tagagctaga gctttgagtc gttgtctctg
      421 ttgttgagct tccaggagtc tggacatagc tggaagatgg aatagttgtt gatggtgtaa
      481 ttgcattcga tctagctgta tattgatctt tgtagtacag ttcaccgccc acacgaatta
      541 atccgcttgg catctcccag tctgtatttg tcacgttagc tgaaacatac tgcataagct
      601 cgcgataaac atcagaggct acgtgtgacg attctgaagt gacaggtgtc atttttttat
      661 tgtaacctgt ccacacggaa atcgaataat ttggcgtata gcccgcaaat aggatatctg
      721 gatacacccc agaagaaata ccaagttttg catactcgtc atctgtgtaa ttggatgttc
      781 cggtcttacc ggcttgatat aacccagcga tttgcgcatt cgtaccagtt ccttcagtga
      841 tcgtatcttt taaaatgtct gtgatcatat aagctgtttc tggagacatg gctgtttttc
      901 catctggctc ataagtttct tctgttccgt cttggaagac gattttatta acatattgag
      961 gtttgtaata agtccctcca ttggcgaaag ccgcataggc tgctgccatt tttaaggaag
     1021 aagctccgta ttttgtccca tcttgttctt ccgtattact agagatcgca ttcgattgat
     1081 ggatcgtact gtattcgatc ccaagatttt ttaagaaaga agccacttta tctgaaccga
     1141 cttcattgaa taatttgact gctggaacat tacgggataa gtaaagtgct tggcgtaaag
     1201 taatggtgcc catatattga ttatcccagt ttcctacagg agtcgaagtc ccttcatagt
     1261 tatacggtgc gtcagtaata gtttttccag tagaatattt caaatattca aaagctggtc
     1321 cgtaatctgt gacaggtttc atcgtagaac caaaatcacg ggaagtattc actgctaagt
     1381 tgttgcctaa agttacgtct tctgcgatgt gacgtccgcc aatctgggct tttactttcc
     1441 cggtattggt gtcgataagg gtggaggcaa cttgcatctc gtcgtctggg taagagacat
     1501 actgatccgt gttgacgata tcatatagtt tcttttgggc gtccagatcc aaattcgtat
     1561 aaatctctaa accgtctgtg tagacattct tatctgtttt ttcttgtact tcattgatga
     1621 cttctttgac ataattatcg acgatcttag tattgtcatc ggattgagtt agttcttgca
     1681 gaccatccgt taccggtaca ttaacagctt gatcatattc tgtttgagag attttttcgt
     1741 tttgaagcat cgtatacaaa acagtatctc gtcttttctt cgcctgatct ggataaacat
     1801 aaggatcata ggcggaaggg gcttgaggca tcccagcaag caatgctgtt tgcggtaaag
     1861 aaagttctga taatttctta ccaaagtaca tctcagaagc tgtctccatc ccgtacaagc
     1921 cgtttgacat atacacttta tttacgtagt aggtcaagat ctcttgtttc gatttttttt
     1981 gttccaatct tacagccatc cacgcttctt gggccttgcg tttcagagtc tgatcctcgg
     2041 cactggtgga gaagaaggaa agtttgatca attgctgtgt caaagtactt ccgccttgca
     2101 gtccgccaga ggtaaagtta gagagggcag aaccgatgat ccgaataggg tcaaccccga
     2161 tgtgtttata aaaacggcga tcttctaccg acacgatcgc atcttctaat gttttcggta
     2221 attcattcgc agaaattttt tcacgctttt ctgcgcctaa atcttcgaat agttcgccat
     2281 cttgtgtata tagtttggaa gatacggtag cgtctaattt tttatccgtc aattcgggcg
     2341 catcttttgc gtaataccag aaaagtccta cacctgctaa aaagaggata cagaaaaatg
     2401 agagtattcc taaaaggatc ttgataaata atcctttttt atgtgttcca gaggactttc
     2461 cagaaccttt gccagaattc ttcttcacac tctttttagg agtaggctgt ttctgtcgtc
     2521 tagagcttct agtttgttca tttgccattc ttgctttctc cttcaataga taatgttgat
     2581 aaatagaagt caaccgcgtc caaataagga atccgcggag aaataccagt tttgatctct
     2641 attcctagtt cttcgatcaa agataaaggt agtgatttgc gtcctttttc cttttgggcg
     2701 tcccagtaag caatcaaatg aacactattc aaataaaaac agcgcttcaa agacgaaaac
     2761 cataacaaga caaagcagac cccattttgt tcgaggcatg ctttcatatg ttcgatttga
     2821 tgggcatgga agtttttaaa gggaaaagat gttttgttct tggtttcttt tgcttcaaag
     2881 tccaagtaaa agcctcgata gacgccatta tagtctgtag ttgaggcttg ggtaaagtag
     2941 gcttccttga tcacagcagc acttctctta ggatagtcta ctttcacgat ttggataggg
     3001 gtaggctttt tatggataac cgccaacccg cgggaaagat aatatttatt actttcattg
     3061 attgcttctt caaagcgcat cccccgattg ccgaattcag tgatctgatt tcttttttct
     3121 tgaatttttt cgacgggaga ggcattctta taataaggtt ggccatttgg atagcgcaat
     3181 accatcttta atcacactcc taggccacat tatagcaaaa aagcggatag aaagaaaagg
     3241 aatctgagat ggataagtca aaagtaatct atgtgtctgg gtatcgaagt ttcgaactcg
     3301 gtgtttttaa agaaaatgat cctaaaatcc aagttgttaa aaatgtttta aaaaaagaac
     3361 tccatcagtt agcagaagaa gggctggaat gggtacttgt cagtgggaat ttgggcgtcg
     3421 aactatgggc tgtgcaaata gtagcggaat tgaaagaaga atatccggaa ctatctcttg
     3481 gtattttgta tcctttttct gattttggta ataactggaa tgaagggaac caagaaaaaa
     3541 aacaattagc agaacaatta gcagattatg tcgaagctgt tagccatcaa ccctaccaat
     3601 cacctagtca gctaaagaat cacactcgtt ttatacttga gcatacagtg ggctgtctgc
     3661 tgatctatga tgaagaattc cctggaaaaa cgaagtattt ttttgaagat gcacagaatt
     3721 accaagcaga tcatccctac gaaattcggc tgatcagtat ggacgatctt caaaatttta
     3781 gtgaataatc gatattgtgt ttgatttatc aacttttttc ctatacaatg aaaaaggtag
     3841 aaatgttatt acgtaaatga aaactagagg tgtaaatatg gcgaatttgg tttatagtcc
     3901 taaagacatt ttacaacaag aattcaaaac aaaaatgcgc ggctatgatc ccgttgaagt
     3961 tgatgaattc ttagacaata tcatcaaaga ttatgaagct tacagcaaag aactccttgc
     4021 tttacaagaa gaaaacgatc gattgagtgc aaaagtagct caattatcta aaactcaagg
     4081 agcagctcaa acacgcgtgc aacaaacaga ggcaccaaag agcgcagcag tgaccaattt
     4141 tgatatctta aaacgcttgt caaatcttga acgtgaagta tttggtaaaa agcttgatca
     4201 acaagcaagt gcagtcaaac cagcacaacc taatccaaat aactacacaa atgcagacac
     4261 aagcttagat gataatgaaa aaacacgtca attttaattt ttgattaatg aatttgcggt
     4321 tttcgggtaa tcgcggtctg acttgatcag gctgaggaaa gtccatgctc gcacaggctg
     4381 tgatgcctgt agtgttcgtg cttagcgaaa ccataagcta aggtactctt tggagtaacg
     4441 gcaggaaaac aagctaaggc ttcggctatg cttaagtatc cttgaaagtg ccacagtgac
     4501 gaagcggctg gggaaaccca tccggtggaa cgcggtaaac ccctcgagcg agcaacccaa
     4561 acaatggtag gggcgctttt tgtctggaag aagaacagga caaaaaggca gtataactgc
     4621 agatagatgg ttaccgcttt gttttttctc cctggaaaag caaagtacag aacatggctt
     4681 atagaaaacc gcaaacatca gggaattagg tcgtgacaaa agtaatgcgt tataagaaac
     4741 aagaaaaaca tttgaaagct atttttcaat acttcagatg aatcgttctt attacaaaac
     4801 aaacttttgt atcaccggtt attcggaatt tgagtaaaag tgtggggtag gactgatgtc
     4861 ccacctcctt tttttataaa taaggagatt gtgtatggaa aaaaacagga agttcaacct
     4921 cgtcgcaact gcggcaagcg ggctagaagc actggttggt aaagaattaa gagatcttgg
     4981 aatcgaatgc caagttgaga acggacgtgc acgttttgaa ggaacaatgg aaacgatcgc
     5041 tacagctaat ttatggttac gtacagcaga tcgagtaaaa atcgtggttg gggaattcga
     5101 tgcgtatacg tttgatgaat tgtttgaaaa cgtaaaagca ttgccttggg aagattttct
     5161 tccactagat gcagcttttc ctgtagctgg aaaatcgatc aagtcgaaac tatatagcac
     5221 acctgattgc caagcgatca caaaaaaagc aatcgttgaa cgtctccgta cctattatca
     5281 ccgtccagct tctgtaccac tgacggaaac tggcgcacat tttcagttgg aagtagcttt
     5341 gctgaaagac catgtgatgg taactctgga tacgacagga ccaagtttat tcaaacgtgg
     5401 ttatcgctta gaaaaaggtg gagcaccatt gaaagaaaac atggcagcag cacttattat
     5461 gttgacaaat tggcgaaaag accgtccatt ttatgatcct gtatgtggtt caggtacgct
     5521 ttgtattgaa gcagccttaa taggccataa tatcgcacca ggattcaatc gtgagtttgc
     5581 ttgcgaatct tgggattggt tcagtcaaga aatcatggaa aatgttcgat cagcagccga
     5641 agaaaaagca gattatgata ttgaattaga tattactggt tcagatatca atggacggat
     5701 gattgaaatt gccaaagcca atgcagaaga aatcggatta ggctcttcga ttacctttaa
     5761 gcaacaagca gtcaaagact tcaaaacgga taaagaatac ggggtcatcg tggctaatcc
     5821 gccttacgga gaacgtctag gagaagaaga aactgttcgg aaattatata aagaaatggg
     5881 cgatgtattc cgtccattaa aaacatggag taaatatatc ttgactagcg acttagcgtt
     5941 tgaggaattt tacggacaaa aagcaaccaa aaaaagaaaa ttgtacaatg gtgcattgcg
     6001 tacggatcta ttccagtatt ggggaactcg tccgcctaga aaaaaagaga aagacgaata
     6061 atcttactaa ttgattcatt caattggatg tttggaggaa tagggaatga aagaaaaaga
     6121 gtttttacaa gaagtaaaag aaatcaatct tctacaacaa gctttaggaa tcctcgattg
     6181 ggatacacaa acaggtatgc ctgaaaaagc tagtgcatac cgaagtgaag tagacagcta
     6241 tctttacagc atttattttt caaaaaaaat cggtccaaaa atcaaagaag ccattcgcta
     6301 tttttctgaa catccggaag aattgtcgga agttggaact gccgtttttg ccaaagtaaa
     6361 agaagagtat gatttggaac atcgcgtacc ggaagatctt atgagaaaat atacagctgc
     6421 cgtttcttca gcacacggac aatggcaaaa agctagagaa acacaacaat tttctgattt
     6481 tcaacacgca ttgaatcaaa atattgaatt aacaaaacag ttgatacctt actggagaaa
     6541 agatgaaaag acgaattatg atgttttgct gaatcaatac gaacccggga tgactgtcga
     6601 aatccttgat aaggtatttg atcagcttaa agaaggaatt ctttcgatcc gccgtacgct
     6661 ccaagaaaaa gggactgaac ctgaaacaga ctttttaaat cggagaatga cgaaaaaaca
     6721 acagaagaga tttgtagtga aagtaatcga acaacttggt tatgatttta gtcgtggtcg
     6781 tttagatgac actgtccacc catttatgac tggaattaat ccgaatgatg cacgaatcac
     6841 gacacgatgg aacgaaacag atttcaaaat ggctgttttt ggagtgatcc acgaggctgg
     6901 tcatggtatg tatgaacaag acatcgatga taaatatgcg tatacaccgg tttatgaagg
     6961 tacatcaatg ggtatccatg aatcacaatc gttgtttaac gagatcatta ttggaagtag
     7021 tcgcagcttc tggaaaaagc aatatccttt ctttcaagaa tgtgcagaag gaacattcga
     7081 tgatattcct tttgacaaat tttatcgttc attgaaccat acaaaatcaa gtttggttcg
     7141 tatcgaggca gacagtttga cttaccctct ccatatcatc attcgttatg aaatcgaaaa
     7201 aatgattttc aatgaagggg tagatgttgc tgaactacct gaaatatgga atcaaaaata
     7261 tgaagagtat ctgggaatac gaccaacaaa tgatgtggaa ggcatcctac aagacgttca
     7321 ttggtcaggt gcaagctttg gttattttcc ctcttatgca ttagggtaca tgtatgcggc
     7381 acaattactt cattctttgc agcaagaaat agatgtggaa aatgttctag cgtcagacga
     7441 ttattctcca atcaaaaact ggatgtcaga gcatatccat caatatggtg catctagaaa
     7501 accaaatcaa ttaatcatgg atgcaacaca tgaaccgctt aatccgcaat acctgatcga
     7561 ctatatgaaa gacctgtatt ttaatgtata tcaggtaaaa taaaatacaa tttacaaaga
     7621 tagctgaaat tcatctaatc tatagatgtt ttcagctatt tacttaagaa agaagtggaa
     7681 cgaatggata aattacaagt gatcgctgat tatagtatgg accaattagc gcatgatcaa
     7741 acaggacatg ggagcgatca taccaaaaga gttgtcaagt tagctgaaaa aattttggag
     7801 acggaaccgc aggcagaccg ctttgtgacg cttgcagcag cttacctaca tgatacgatt
     7861 gatgataaag tcgttaaaaa tgagaacgag gcaaagcaac agcttcgtgt attccttcgc
     7921 tctttgccaa tagtagagga acagatctcg atgatttttt ccattatcga aaatatgtcc
     7981 ttttctaaaa atctctctga agcggccgaa ctatctctgg aaggtaagat cgttcaagac
     8041 gctgatcgta tcgaagcatt aggagcaatc ggcattttgc gtactgctta tttcggcggt
     8101 ggacatgggc acccgatttt tgatcctgaa ctgtatccgc aaacattcaa ggacaaagaa
     8161 aattaccgaa aaggcacgac agtgatcaat catttttatg aaaaactgtt cttgctgtct
     8221 gataaaatga atactgatta cggatatgaa gaagccaaac gaagagaaac cttcatgcgt
     8281 gaattcttag aagaattttt tctggaatgg tctattgacg atgaaaattt tactccagac
     8341 aattggatca aaaataacaa aaaatgatgg aggttgtgac agaagtggtc agctccaaga
     8401 tatttcggat cattttccgt aaaagttact tctggaacac tgtttaccaa gaggtcgtga
     8461 gcctgccgtt cagctccgag tcacaaggaa caatttcaaa aaatagcttc tcgtgtttga
     8521 tgaatattgt aacttgtgat tcggaattgg caggagaaca ccgtttattc ggaagaatag
     8581 gactgtgaca agacttttgt cacagtcctt ttttcgaatt caacaagtaa aacttttaag
     8641 aaatattcag agagtaaaat taaaaattgc tgataaacga agatggaaat tttatgtatt
     8701 tttattggtc tatgcaataa ataaattaaa tattcagaat acaattgatt ttaacgaaag
     8761 ggcttctatc aatggttgtg ttgtatttat ctgcccaggt agagtaaaat agatcattat
     8821 ctaaaaggta attgttcata ctatataagt catttgaaag taaaaagata ctgtaaataa
     8881 aaaatgtggt taaataattc tcataatcag tgtaagcgtt atcaaaaata tagcatactt
     8941 gctttgtaga tgaatgacac gatatatgtt tggtatgttc tgcaagacag atgaatatgg
     9001 aggtggagac aatgtcttcc aaagaactaa caatgtattt agagaaagaa ttggatcaac
     9061 tgaaagaaaa ggggttatac aatacgatcg acatactaga aagtgaaaat ggtgcttgta
     9121 tcatcgtaga cggaaaaaag atgatcaatc tagcatccaa caattatctt ggatttgcca
     9181 atcgcgaaga attgaaaaaa gcatgtattg aagcaaccga aacttatggt gtaggagcag
     9241 gagcagtccg aacgatcaat ggttcgctga aaatccatca gcaattagaa gaaaaaatcg
     9301 cagaattcaa agggacagaa gcagcaatcg cctttcaatc tggattcaac tgcaatatgg
     9361 gcgctatctc tgcagtaatg acaaaagaag atgctatctt atcggatgaa ttaaaccatg
     9421 catctattat tgacggttgc cgcttatccg gagcaaaaat catccgaatt aaacatcaag
     9481 atatgaaaga ccttgagaaa aaagcaaagg aagcgataga aagtaagaaa tataaaaaaa
     9541 tcatgtatat cacagatggt gttttctcca tggatggaga cattgcacga ttgccggaaa
     9601 ttattccgat tgttgaaaaa tatggactga tcacctacgt cgatgatgcg cacggctcag
     9661 gcgtaactgg aaaaggtgca ggaaccgtca agcattttgg attaagcgac aaaatcgata
     9721 tgcaaatggg cacgttatcg aaagctatag gtgttgttgg gggttatgta gctggatcga
     9781 aaacactaat cgactggttg aaagctagaa gtcggccatt cctattttct acttcgctaa
     9841 ctccaggtgc agctgcagct gcattggctt cgattacttt gatgcaagaa catccggaac
     9901 ttgtcgaaaa agtttgggaa aatgctaatt attttaaaga agaattgaaa aaagtaggct
     9961 ataacatcgg tatgtctgaa acacctatta ctccagtcat tttgggagac gaaaaagtga
    10021 ctcagacttt ctctaagaag ctgatcgaac atgggattta tgctaaacca atcgtttatc
    10081 caactgtgcc tcttgggaca ggcaggatca gaaatatgcc gactgcggaa catacgaggg
    10141 aaatgcttga tgaagcagta gcagtttatc aaaaaatagg aaaagaaatg gaaattattt
    10201 aactaaattt agttttttcg tctatttaaa attagagaac atgttcacgc atatattgtt
    10261 aatcatctga agatagtagt aaaaaagatc agggaggatc atcatgaaaa aagtattggt
    10321 gacaggatgt ttaggacaaa ttggttcaga gttgatcaca cggctacgtc aagaactagg
    10381 cagtgaaaat gtcattgcga cagatatacg catgcctgaa gtcattgaga aaaacgatat
    10441 ctttgagatc ctagatgtaa tggattacga aaaaatgcgc agcatagcag aacaatatca
    10501 agtggacaca ttgattcatt tggctgctct tttgtcagct gttgcagaag caaaaccaca
    10561 attagcctgg aatttgaacg tgactgggct ggtcaatgca ttggaagtcg ctagagaatt
    10621 ccagctgaaa ttttttacgc caagttcaat cggcgcgttt ggtccaaata ctccaaaaga
    10681 tcatacccca caagatactc ttcagcgtcc tacaaccatg tatggggtaa cgaaggtagc
    10741 tggagaacta ctatgtgatt attattatgt gaaatatggc gtagatactc gtggtgtccg
    10801 tttccctgga ttgatttctt atgcaacctt gccaggaggc ggtacgacag actacgcagt
    10861 cgatatttat tattcagcaa tcaaagaagg aaagtacact agtttcattg atcaaggtac
    10921 atcaatggat atgatgtata tgccagatgc gatcgatgcc attataaaac tcatgaatgc
    10981 agatcctgat cgattggttc atcgaaatgc cttcaatata tcggcgatgt cttttgaccc
    11041 ggaagagatc aaagcatcta ttcaaaaaat cataccagat tttgaaatgg attatgatgt
    11101 ggatcctgta cgtcagtcaa tagcagaatc ttggccaaat tcactagatg tttcttgtgc
    11161 gcaagaggaa tggggctttt cgccgaaata cgacttagat gtgatgacaa aggatatgtt
    11221 ggaaaaactg aaagaaaaat tggtagagac ggtataaaac aagaaccaca tccaaaaagt
    11281 tttttatact tttgggattg gctatggtat gaaaggggac aagggatgga agaatatcaa
    11341 gccacgccag taaaagaagt aaaaatagaa aatgaattaa aacgtacgat gggatttttt
    11401 actgctttat cgactgttat gggcacagtg atcggtgcag gggtattttt taaagccgca
    11461 agcgtagcag aagttacagg atccgccagt ttgcatatgt tttcttggtt tctcggtggt
    11521 atgatttctg tctgtgcagg actaacagga gctgaactgg cagctgctat tccagaaaca
    11581 ggagggatga ttaaatatat cgaacggatt tacggaaata cagcagcttt tttattgggc
    11641 tgggcgcaag tcgtgattta ttttccagca aatgtagcag ccttgtcgat cattttcggt
    11701 acacagttcg tcaatctatt tggtctttcc cagtcaatga ttgttcccgt agctgttaca
    11761 gctgctgtat cgatcctgtt gatcaatttt ttaggttcta aagctggagg ggcttttcag
    11821 tcgattacgc ttgtctgcaa gcttattcct ttgtttgtga tcgtcatttt cggtttgttc
    11881 cgacaagaag gagtagactt ccaactgttt ccgatccaag caggagaaaa tctttcattt
    11941 ttctcagcct tgggagcggg cttgctagca acgatgttcg catacgacgg ttggatacac
    12001 gtaggaaata tttcgggaga attgaaaaag ccggcgaaag atttgccaaa agcaatttct
    12061 ttaggaatca tcggaatcat gatcgtttat ttattagtaa atgcggtgtt cttaagaacc
    12121 gcatctatcg acggagtcgc aggaaacagc aatgcggcaa gtgacgttgc aaaaatgatt
    12181 tttggcggct tcggtggaag actggtcaca gttggtatct tgatttctgt ttatggaacc
    12241 atcaatggct atacccttac tggaatgcgt ctcccttatg ttatggcaaa agaaaacaac
    12301 ctgcctttta gcaagctctt tgctaaactt catgataaaa ctaaagttcc tgtagccgca
    12361 ggtatcttag agctagtcat cgctattggc atgatgatga tcggtggttt tgatacacta
    12421 acggatatgc tgatctttgt catttggatt ttttatacga tggtctttgt tggtgtcatt
    12481 cttcttcgta aaaaagaacc tgatttgttt cgaccataca aagttccaat gtatccgttt
    12541 attccactag tagcaatcat tggagggact tttatcgtat caagcacgct tatcactcag
    12601 acctttttag cttcgatggg gatagtattg acacttgctg gtatcccgat ttatctctat
    12661 ttaaaacgta gatagctaaa acattcgaat tttattagaa ctgcacttct ctgccaaaca
    12721 aaatatttta tttaaatgag gagtacgtgg aagaaatgtc aaaagtactc gttaattttg
    12781 atcacgtaaa tgtcgttttg tctacgatga aatagcaaag taaaagacag aaatcgggct
    12841 caaccgattt ctgtctttta ctttgatttg cgtaactgaa ctattcggcg tacgccgcga
    12901 taaatattta ataaaaccaa gcaaccagaa atgaaaatgg gaagccaaat cagatagaca
    12961 gggtagccaa gcactttaga caaagcatgt ggagtcattt tatccatttc aagaagtgct
    13021 tgctgccaag tctgaagatt gaagactagc atgaaacccg aaataatcag gagaataaat
    13081 cctaaataat gaattgtagt tctttttctt acataagcga tcgtagcata aaggaacagt
    13141 aaaaaaatag gtacgccaaa aacaaagtac atattcagca tctcgacacc ccctttgtgt
    13201 ttatcttact ttactttgaa agaaatgaga aataatagcc tcgttacttg cttgaaacag
    13261 caaaggactg ctaagataag agagatacaa agaatcaagg aggagtcata tggcaagtta
    13321 tatcgaaccg attcgttttg cgctgattgt ttttccattt ttagccttag cgatctcatc
    13381 tgtttttttt atttatgaat atcgaaagta tggaacattt ctagtaacaa gggcagttat
    13441 tttgtattct ttcgtttttt atttattgtg tgcgtatttt ttggtgatct taccacttcc
    13501 ggcaaaggag acggttgccc aaatgacagg gccaaagatg gagctgcatt tatttgcttc
    13561 atggcaacat ttcagagaca acacggtatt ggtcttgtct gaacctagta cctacctgcc
    13621 ggcaatgaag cagagtgtat ttttggaacc agcttttaat gttttattac tcataccatt
    13681 tggcatttat ttaagatatt atttccggct ttcttttatg aaaacgatga ttgctagttt
    13741 ctgcctttct ctattttttg aattgacgca gctaagtgga ctttatttta tctacccaag
    13801 accataccgc ttatttgatg tgaatgattt agtgacaaat acattgggtg gaatcattgg
    13861 ttttgtcgtt acgccgttat ttacatttat gcttcctaca agaagacgaa tggatgaact
    13921 ttcttatgaa aaaggaaaga ctgtctcatt gactcgacgc ttggtcgctt atttgatcga
    13981 ctggacattt ttatctctta ttaatttggt agcggcgatt ttctttaaaa ttgcgttcga
    14041 ttcaggggca gttacttctc atatctggtg gcttttatta gaagtagggg tatactttat
    14101 gattgtaccg tttataacca atggatggac acttggcaaa aaaattgtgc gtatccgttt
    14161 ggtggaagaa gaccgcgaac gaatttcttt aaaagcattg atgatccgct acgggtatct
    14221 gtactttctt ttttatggtt taagctggtt ggggcagttg cttcagcaaa tcatgaattc
    14281 gacaacgaat acggtgttgc agatgagtgc agtattcatt tctctcggtg tcttgttact
    14341 acaaggtatt tttattttaa atatcttgct atcatttttg agaaaaaaac ggattctgtt
    14401 ttatgaaaaa gctagtcaca catatactgt tagcacgatt ggccaaacaa aggatgatga
    14461 ttctcgaaat tagctgagca aaaacgcttt gtctgtgtta taatgccaaa gtcgtgataa
    14521 tttaaagaga agaggtgcac gaatgaataa ttttgatata ttccgcttca aaccgtttat
    14581 caatgaagcg ttaaccgcaa agggcttcaa acagcccaca gaggtccagg aacgtttgat
    14641 tcctttaatt gcaaaaggaa aaaatatcat tgggcaatct caaactggta caggaaaaac
    14701 acatacgttt ttgttgcctt tgtttgaaaa aatcaatgca gaaaaagagg aagtacaagt
    14761 tgttattaca gctccaagta gggaactagc tgcacagatc tatgaagcag ctacacaaat
    14821 cgctaaattt tctacaccgg agatccgtgt ctcgaatttt gtcggcggta cagacaaaca
    14881 gcgtcaatta aatcgtctgc atcaccaaca gccacaggtt gtagtaggca cgcctggtag
    14941 aatattagac atgatgaatg aacaagcgct gaagattcac accgcatttg catttgttgt
    15001 cgatgaagcg gatatgacat tggatatggg ttttttaaat gaagtagacc aaattgcaag
    15061 tcgattacca gaaaagctgc agtttttagt tttttctgcc actattccag aaaaacttcg
    15121 tccattctta cgaaaataca tggaaaatcc aatcattgaa gaaatcaaac caaaagcagt
    15181 tatttctgaa gacattgaaa attggctgat ttcaacaaaa ggtaaagatg agaatcaaat
    15241 gatttatcaa ctgctgacaa ttggtcatcc ttatttagca atcgtatttg ccaatacaaa
    15301 acagcatgtg gatgaaattg ccgattactt gaaaaaccaa ggcttgaaag tagcaaagat
    15361 tcatggagac atcaccccaa gagagagaaa acgagtgatg cgacaagtac agaatctgga
    15421 gtatcaatat gttgtagcga ctgatttagc tgctagaggg atcgatatcg aaggtgtctc
    15481 tcatgttatt aatgcagaaa ttccagaaga tctagacttc tttatccatc gtgtcggacg
    15541 tactggacgt aatggcttaa aaggaacggc cattacgtta tattcgccaa atgatgagca
    15601 agccattgat gcgatagaaa aattgggcgt ctcatttgaa ccaaaagaaa tcaaaaacgg
    15661 ggaaatcgtc aaaacatacg atcgtaatag acgtgccaaa cgagaaaaat caaaagatgt
    15721 attagatccc acgctgattg gtcttgtgaa gaagaaaaag aaaaagatca agcctggtta
    15781 taagaaaaaa atcgattggg caattgcaga aaaaaataaa aaagaacgca aaatcgaacg
    15841 cagacagcaa agccgtactg ctcgtaaaaa taagaaaaat tcacataaat aaaaaacaag
    15901 aggttgtgac aaaaggcgtg tcacaatctc ttgtttttaa gctagacttt ttttcattcc
    15961 gatacaaagt acttcatctt caataaaagg ttctgtgcaa gtatgatacc cttgtttttc
    16021 ataaaaatct acagccgtca tttcagctac tacataacta gtctgacaac cttctgaaac
    16081 cgcccgtttt tcagcagctt caagcaactg accgcctaac ccttttcctc ggtattcttt
    16141 tgctacgcaa aatcgttccg gcttgaagca agtaggtgtt ttcttttcat aacgcaaagt
    16201 agctactggt cttttttgat caagaaatag ataataggtt tgttgcggga tatcttttcc
    16261 gtcgaattct agttctgcag gaatcccttg ttctaataca aaaacttgat atctcaaaaa
    16321 gcagctagct gcgcgtatcc attcatccgc tccaactatt tttttcatca tattcctcct
    16381 tgtcatgaat agcagagatg tattgattcc tttattctta tcgtaccaaa aatcgtggag
    16441 tatgaccaaa aggaaatgaa aataaaaatt gagatattga cagtgatcaa tagaatgcgt
    16501 atactaatta agtacgaaaa ggatatgatt tgttttttaa gactcacaga aaattctttc
    16561 ttggctgaaa aaagaatagt cactaatcaa attgctccct ttttctattt tggtagaaat
    16621 accgacgcag ctcagcgtta tcgagtttaa gaggtaatga ttacacatca ttgcaatcaa
    16681 ggtggtaccg cgagttttcg tccttggctg tgatggtgtt tttttgtatt taatttatag
    16741 aaagcaggta atgacatgaa acaattaagt agtgcacaag ttcgacaaat gtttcttgat
    16801 ttcttccaat ctaaaggaca tacgattgaa cctagtgcgt ctttgatccc cgtgaacgat
    16861 ccaaccttat tgtggatcaa ctctggtgtt gcaacattga aaaaatattt tgatggttca
    16921 gttgtgccag aaaatccaag aatcacaaat gcccaaaaat ctattcgaac aaatgatatc
    16981 gaaaatgtcg gtaaaacagc acggcaccat acgatgtttg agatgctggg taatttttcg
    17041 attggtgact atttcaaaaa tgaagccatc cactgggcat gggaattttt gactgatcca
    17101 aaatggatgg cattcgatcc agaaaaactt tatgtaacag tttatccaaa agatacagaa
    17161 gcaaaacgca tctggcatga agaagttggc ctgccactag atcacattat tgatgtagaa
    17221 gacaacttct gggatatcgg tgcaggccca tgtggtccgg atacggaaat tttctatgac
    17281 cgtggagaag cttataacga tttagcagaa gacgatcctg aaaactatcc aggaggagaa
    17341 aatgaacgtt atttagaaat ctggaactta gtattttcag aattcaacca taagcctgat
    17401 aacacatttg aaccacttcc tcataaaaac attgatacgg ggatgggatt ggaacgcatg
    17461 gtctcaatcg ttcaagatgc gtcaacgaac tttgagacag acttgtttat gcctattatc
    17521 cacgccgtag aaaagctaag caaacaagta aaatatggtg aaaacccagt aacagatatt
    17581 tcttacaaag taatcgcaga tcatattcgc gccctttcat ttgctattgg cgacggtgca
    17641 ttgccatcga atgaagggag agggtatgtc ttacgccgtt tgcttcgtcg tgctgtcatg
    17701 catgggaaaa aattaggtat tgaacaagca ttcttatatc gtttagtccc tgttgttggg
    17761 gaaatcatgg taagttatta tcctgaagta cttcaacaaa aagaatttat tgaaaaagtt
    17821 atccgtacag aagaagaacg tttcaatgaa acaatcaatg aaggtttgga aatcttaaat
    17881 caagtgatcc aagaagtaaa aaacgaaaac gggacgactc tagcaggaaa agacatcttc
    17941 aaactatacg acacatatgg cttcccggta gaattaacgg aagaggtggc agaagatgaa
    18001 ggactgaaag ttgatcatgc tggttttgaa aaagaaatgg atgcacaacg cgaacgtgct
    18061 cgctctgccc gcagtactga aaaatcaatg ggggtacaat ctgctttatt gacagatatc
    18121 aaagtagaaa gtaaattcat tggatacaca gaacaagaat cagatagtca attattggtc
    18181 atcgttaaag gtgagtctct tttgaatgaa gtcgcagaag gtgaagctca aatgatcttt
    18241 gatcaaacac ctttctatgc agaaatgggt gggcaagtag cggatcaagg atggatcatg
    18301 aatgaagtag gcgaaatcgt agcagttgtt acagatgttc aaaaagcgcc taatggtcaa
    18361 tttatgcaca ccgtagaagt aaaagcacca atgagtgaag gacaaaccta tcatctattg
    18421 atcgatcaat cgatgcgcaa tcggatcatc aaaaaccata cagcaaccca tctactgcac
    18481 aaggcattga aagaagtact tggcgagcat gcaaatcaag ctggttcttt agtcgctcct
    18541 ggacatttac gattcgactt tacccacttt ggtcaaatca cttcagatga attagctgaa
    18601 atggaaagaa tcgtcaacga aaaaatctgg gaagctatcc cagttgaaac aattgaaaca
    18661 gatatcgata cagccaaaaa catgggtgca atggctttgt ttggtgaaaa atacggacat
    18721 gacgttcgag tagtaaatat tggcggctgg tcaatcgaac tgtgcggagg cacacatgta
    18781 gcaaatacag aaaacatcgg aatcttcaaa atcgtttctg aatcaggaat tggtgcaggc
    18841 gtgcgacgaa tcgaggctgt aacaagtaaa gaagcgtacg aactacttga agaagaagaa
    18901 aaacaactaa aacaagtagc aacaattgtt aaatcacctc aattgaaaga agttgtttca
    18961 aaagcggaac aattgcaaca gcaagtaaga gagctacaaa aggaaaatga acaattagct
    19021 agcaagttag cgaatcaaca agctggtgat gtattcaaag atccagaaac gatcaatgga
    19081 aaaacaatca ttgcagcaca agtaaaagtg aaagatatga atcagctgcg tcaattagct
    19141 gaccaatgga aacaaaaaaa tatttcagat attcttgttt tagcaaacga acaagaagga
    19201 aaagtcagtc tgctagcggc aatgacgaaa gaatcaaatg aagctggact aaaagctggc
    19261 gacttgatta aagcaatcgc accgaaagta ggcggtggcg gtggtggacg tccagacatg
    19321 gcacaagctg gcgggaaaaa tcctgctggt attccagatg cacttaaagc tgcgaaagaa
    19381 tgggttgcgc aacaataggg cgtttacgtt tgacagaaaa acaaaagttc aaacattgaa
    19441 cgaaacatga ttatgggggc gaagacaaac aagtcttgcc ctcatacttt tttcaattgg
    19501 atggcgcatc taatcaaatg gtcggattcg gatggtcttt agttgacgaa agtgacagaa
    19561 atttttacga ttagtataaa taaaaagggg tgaaaaaatg aatagtagaa ataagacctt
    19621 tcgactagtc ttacgtgcga tcttgttagc aatcatcatc gtacaagcga tggttccttg
    19681 gctggggttt attccattag gattcattag tttaacaatt atccatatca cagtaattat
    19741 tgctgcagta gtattaggcc caaaagatgg catggtgatt ggcttgtttt ggggaatcgc
    19801 tacaattgtt cgtgcctatg ccatgccaac aacgccattt gatacattag tgttcacgaa
    19861 tccgattatc tcggtagttc ccagagtact tgttggctta gtagcaggct tggttttcca
    19921 ctggatttat caaaaaaata aatcaataac gatcagctca gcctttgcag gggtactggg
    19981 ttcattggtc aatacagtct tagtgttagg attcatggga ttattttata caggtgcaac
    20041 ggcagatgcc tatggtgtag actctgctct tttatttaaa accttagcag gggttgcagc
    20101 aatcaatggg ataccagaag caataggtgc gggaatcata acaccattac tagcaaaagc
    20161 gttatttgca gcaacaccgt tgaaaccaga ataatgacca tcttttaata gtaataggac
    20221 gcacgataga tagaaaaagg aagagaggag aggaaaacaa tcgtcctctc catcttttta
    20281 cctgagcaac aagcaaacta gaaggggtaa aaaacttggc taagaaacaa gaagaaaaaa
    20341 agaaacaaat accgcaacag cggacaaaaa aaggagtact agcgggtatc ttaggattgg
    20401 ttacgaatct gatcttgttt gtctctaagc taatgatcgg gcttttttca ggaagtgtct
    20461 ccattatggc tgatgcagtg aacagtctat cagatactgc ttcatccata ttgactttga
    20521 tcggatttaa aattgctgca aagccagcag atcaggaaca tccttatgga cacgaacgct
    20581 tcgaatatat cagtgggtta tttgtttcga tcatcattac ttatgtagga tttcaatttt
    20641 tagagtcttc tgctgaaaaa atcatccatc cagaaaatgt ctctttaaca cccatcgtct
    20701 tttttgtcct aatcatttcc atctttttga aatttgcaca aggcaaaatg tatcaggtta
    20761 ttgcgcaatc cattgattct gaaacattga gagctacagc aacagatagt tataatgatg
    20821 tctttactac actggctgta ttgctttcag cagcaatcga atgggtaact ggatggcgta
    20881 ttgatggata tataggtttt gctttagcat tatatatcct ttatagtgga ttgacgatgc
    20941 ttcgaaattt tgtatacgaa ctattaggaa gtcgcccaac gccagcagag attgaagaaa
    21001 tggaagagca tttaaatgct tgcgatgata tacttggcta tcatgattta ttggtgcata
    21061 actatggacc caataaacgc tttgcatcag tccacatcga agtagatgaa tcaatgaatt
    21121 tgaatgtggc tcataagatt attgatctga tagaaaaaga ctttaaaaag tctttggatg
    21181 tggaacttgt ctgtcacttg gatccaatgc ctgttgataa cgaaaaatac tataatatct
    21241 tgcggcagtt aaagcaaatt cttcttgaaa caggtacagg gcttaatatt catgatttta
    21301 gagtaattca tcaaggaaaa acgctgcaat ttgatgtagt ggtccctgag aaattccagt
    21361 ttgttgatac agaactagaa agaatcttac gtagtcaaat agaagaaaaa atcggagatt
    21421 accaattgga aattacgttt gatcacaatt atcttttata ttaaatgaga aactataaaa
    21481 agactcctta aagtttttaa tttaaagagt ctttttttct tttaattgtt tatggagagc
    21541 ctgacgtgca agattatctg ctcctttatt tttgttttcg ggcaaccatt gcaggatcaa
    21601 taaagaaaat tcggatagaa gttcttggat tctttgaagg tagggaagga agagctcgtt
    21661 cttcgttgcg tttttgtcga ccgtagaaac tagtgttttg ctgtctgaaa aacagaaaat
    21721 cgtttcattc tgtaagtcgt ttttctttag aaattctaaa gtttttaaaa agacttcgaa
    21781 ctctgcttga tggtttgtcc ctatagaaag cgggatagat agctgttctt gtttaccttg
    21841 atagacaatc aagattccac ctccactagg accagggttg ccttttgtag aggcatcaat
    21901 gtatactttt aacataaaaa gagattctcc tttttgttca aactaatgtt atggtaagat
    21961 tatatcatta ctgaatcggt gagaggttag agaatgaaaa aaaatattcg atggcagcct
    22021 gaactggcac atacgatcat ctattggtca ttgaccttga tcattctgtt ttttagtctg
    22081 attctttctt tagaaaacac aggtccttat tggaaaagca attttgtttt aggtattttt
    22141 cttgtttttc tatttctagg tttgcggcgt tcttttattt taaaatcaaa ctctgtgatt
    22201 atacgttatg ctgctttttg gaaagaccga gaaatatctg tcggacagat tcgatctttg
    22261 acgccaacta atactggggt gcaaatgatg ttgcaagatg agaaagcccc atctgttttc
    22321 atgatgcgaa aaaaagatca agtagtcctt tttgatcatt ttgaaaaaaa cggacccaaa
    22381 tcagaatcaa ttccttatcc ttatacgaaa aaaagaaaaa gctaatttta aaaatcagaa
    22441 cctaaaaaaa cgaattttta caggaaaaga aaagagtgag acgtacggtt catcccgttt
    22501 agcgtctcac tctatttgtt tatctattca tgcatactag tatcggtagt ctgaacgttg
    22561 cctttcttga catgggcagc ttgtgttcct cgattccctt cgatgatctc aaattcgacc
    22621 acttgatttt cttccaaagt tttgaatccc tcttcttcaa tcgcagtaaa atgtacaaaa
    22681 atttcttctg tgtcatcata actgataaag ccgtatcctt ttttgttgtc aaaccatttt
    22741 acaattcctg tggtcaaagc actcacgacc tttctaaagt ctgatcatag ccttcattat
    22801 aaagagtatt tataagtacg tcaaataaat taaaaacctg taattttatg ttttttttct
    22861 gaataaaaaa aggtataatt ggcatatctt aacaaagaag atattttgtt gaataaagtt
    22921 cgtgaaaagg ggtaatcaaa tgaagaatga tctgcaaatt tcacaagaaa cagagctaga
    22981 accgattgtt gaagtggcta gtaaattaga tcttaaggaa gacgatttgg aactgtatgg
    23041 gaaatataag gcgaaaatca atttttctgg aatcaatcga tcaaaggata acgctgaggg
    23101 gcatttgatc ttagttactt ccatcaatcc aacaccagct ggcgaaggaa agtcaacgat
    23161 cactgtaggg ttaggagacg cgctcaataa aatcaataaa aaatctgtta ttgcattgcg
    23221 agaaccctca cttggtcctg tgatgggaat caaaggtggt gctgcaggcg gaggatatgc
    23281 acaggtattg cctatggaag atatcaactt gcattttact ggtgacatgc atgctatcac
    23341 aacagctaat aatgcattat ctgctctttt agataatcat attcatcaag gaaatgaatt
    23401 gaacatcgat gcccgccgag tgatttggaa acgggtagtc gatctcaatg atcgtgaact
    23461 gcgtaaagta attgttggct tgggtgggcc aatccaaggt gttccaagag aagatgggtt
    23521 tgatatcaca gttgccagcg aaatcatggc tatcttgtgt cttgcggcag accttgaaga
    23581 tttaaaggct cgtcttgcaa ggatcgttgt cggctataca tttgaccgtc agcctgtgac
    23641 tgctggagac ttgaaagcag aaggcgctct ggcattactg ctgaaagatg caatcaagcc
    23701 gaatcttgta caaacaattt acggaacacc agcttttgtc cacgggggac cgtttgccaa
    23761 tatcgctcat ggctgcaata gtattttagc taccaagaca gcattgagat tagcagatta
    23821 cgttgtcaca gaggcaggat ttggtgcaga tcttggcgga gagaaatttt tagatatcaa
    23881 agtacctaac ttgaaaaaag cgccggatgc tgtggtgatt gtcgccactg tccgtgcatt
    23941 gaaaatgcat ggcggaatga aaaaagatga acttaaaaat gagaatcttg atgctttgaa
    24001 agtaggtttt gcaaatttaa agcgccatat acgtaatatg gaacaatatc agctacctgt
    24061 gatcgtcgca atcaatgaat tcgtaacaga tacagacaat gaactcacac tgttggagca
    24121 cttgtgtgaa aatcagggga ttcttgccaa gagagcgagt gtatgggcag atggagcaga
    24181 aggcggcgtt gatttagcaa aagctgtagt acgattgatt gatagaaaag aagctaatta
    24241 caagccatta taccgcctag aagaaacgat acaagaaaag acagaaacaa tcgtcaagaa
    24301 aatttacgga ggaaatggtg ttgttttcag caaaaaagcg caaaaacaaa tagaagagtt
    24361 tacgaagaat ggatgggaca aactgccgat ttgtatggca aaaacacagt attcattttc
    24421 tgatgatcct tcactgctag gtgcaccgga agatttcacc atcacgatcc gcgaaatcat
    24481 cccaaaatta ggggcaggat ttttagtagc cttgactgga gatgttatga cgatgcctgg
    24541 tttgcctaaa aaacctgctg cattgaatat ggacgtgaca aatgacggag aagtgttggg
    24601 attgttttaa gcttgattat aatctaccat cgaaggagct gtattttgac tacagcttct
    24661 ttttttagac gatagggaat taattttgtt tacactagga tcattctttt tctttattgt
    24721 attttgagct atactaaagc ataataggag ggtatgatga aaacgacatt tataagaaaa
    24781 accggttttt acggtatggg tagctcgatt cagctaagaa tcaacaatga aaaacgcagt
    24841 gttctcagtc atgaacaaaa gataacgctt gatctggaag tgccctttac gatgcaggtc
    24901 accttttatt ggctaaaaag ccccgtttat acagtgaaag aagcaaagcc tgcttatgtg
    24961 atcacaatga attacttgat tcttcaaatc tatccgtttt tatttctagc aactgggttg
    25021 ggggccgttt tcctacaaaa tttatttttc agtttttttg cagttattgt gttgattgcg
    25081 ttctttttct atataaaaaa ccgggcatat ttgatcaagg aggaaaatta tggcaaactc
    25141 tgatcttttt ttaatcagtt tcaatcgaat cgaaaaatgg atgagagacg agatgggaaa
    25201 tgcacgaaat atgggattta ccgaacttgt cagacgccta gctcaaagaa aacaattaat
    25261 gatcagaaaa tacgaagatg atcttttaca gcttgctcag ttaaggaatg cgatcgtcca
    25321 tgatcggatc gcagtcgatt ttgtcattgc agagccaaat gattggacag tcaagcgaat
    25381 ccagctgatc gaacaagaac tgattcagcc tgaaaccgtg ctgcctcgat ttgcaaaacg
    25441 tgtaacaggt tttgaacaag atcttccgtt acttgaactg ttaaagatag tagcagaaaa
    25501 aaggtattca caatttccgt tatacaataa aggaaaattt gtagcattga ttactttacg
    25561 taatatcggg ttttggctag caaaagaaag ccaaaaaggt ccggttgatt taacaaataa
    25621 acgagcgttg gatttgatca tccaaaacgg aaagtacaca aattatcatt ttgttccagc
    25681 taaaacacat atttacgagg ttgaagcgat gtttcgtgag caagggactt tggaagccgt
    25741 acttatcact aaagatggga atcccgatgg taatctgctc gggatcgtac gacctcgtga
    25801 tatttataaa caaatagaaa aggattgatt ttttgttaat tatttattgg atattcagcg
    25861 ctttgctagt aggagtggat cagttggtca aatggtggat cactgataat ctagcgcttg
    25921 gagagacgaa aaatcttata ccaggtattt tgtcactgaa tcatattcga aacactggag
    25981 ccgcatggag tatgctagaa gggaagatgt ggttctttac tatagtgaca ctcattgcag
    26041 tcgttgtgat tctcacattg atgattaaaa atcgtgaaaa aggtaatcgc tggttcatga
    26101 ttggattgag tttgattcta gctggtgcga ttggcaattt tatcgaccgt attcgtcttg
    26161 gctatgtagt agatatgttc cagacagatt ttatgagctt tccgattttt aacgtagcag
    26221 atgtgacact tgtatgtgga gtgatttgta tgttaattta tattatttta gatgaaaagg
    26281 atcaaagaaa aaaatgacag aacttaaaac gacgattcac catgaatctg gtaggatcga
    26341 taaagtattg actaatttat tcaccgatta cagtcgttcg caaattcagc tgtggctgaa
    26401 aaatggagcg gtttctgtca atggacagcc agtcaaagca aattacaaag taaaacaagg
    26461 agacgagatt actgtcttga tccccgaacc agaaacatta gatgtggtcc cagaaaatat
    26521 cccgttgacg atcgtttacg aagatgaaga tgtagcggtg ataaacaaac ctcaaggaat
    26581 ggtcgttcat ccttcagcag gtcatacgca tggcacaatg gtcaatgcgc tgatgtacca
    26641 tatcaaggat ctttctacga tcaacggcgt tattcgtcca ggtattgttc accgaatcga
    26701 caaagatact tccggactgc taatggttgc caaaaatgac caggcacacg aatcattagc
    26761 tcagcaatta aaagataaga cctctttgcg caaatatgtt gctttagtcc atggagagat
    26821 tcctcacgaa aaaggaacca tcaatgcgcc aatcggccgt tcgaaggaaa accggaaaat
    26881 gcaggcgata agagaagatg ggaagtcggc tgtcacacat tttactgtat tggaacgttt
    26941 cgaaggattt actctggttg aattaaaact agaaaccgga agaacacacc agatccgtgt
    27001 gcatatgaag tatatcggtt atccgttagc tggagatcca gtatacggac cgaagaaaac
    27061 tttgaaaggc aatggtcagt ttctccatgc gcaaatgtta ggttttacgc acccgactac
    27121 aaacgaaaag atgatctttg aagcacctct tccagaagtt tttgaaaaaa ctttagaaaa
    27181 attaagaaaa aacattgcat tttaatttag ataacggtat agtaattaac aacgaaagca
    27241 taaaacttaa tagaattcct ttaagtttag tcccgtgagg ctaagaaggg tgggagcaag
    27301 gatacgtatg gtgcgccata agtagattgc tcattgacat agtatagcta tcagtgtatc
    27361 agtatacgct cagtctaaac attcctcctg tcctcttagg gcaggagttt ttttatgcaa
    27421 aaaagtaggg ggaatgaaaa tgcaagcaaa agaagtagta gatcaggtaa ccatgaagcg
    27481 agcattgact cgaattactt atgagattat cgagagaaac catagtatcc aagatatcgt
    27541 attggtaggt atcaaaacac gaggaatcta cattgcatca agaattgccg aacgcctgaa
    27601 gcagctagaa gatatcgata ttcctgtagg agaactggat atcactttat accgagatga
    27661 caaaaaagaa aatccagaag aaccggaact tcattcttct gacatcccag tttccttaga
    27721 aggaaaagaa gttattttga ttgatgatgt tttgtataca ggacgaacga ttcgagcagc
    27781 aatggacgca gtaatggatt ttggcagacc aagaaaaatt tcattagcag tcctggtcga
    27841 tcgtgggcat cgagaattgc caatccgtgc agattatgtt gggaagaaca ttccaacatc
    27901 tcgggcggaa gagattttag tagagatgca agagcttgat ggacaagatc gcatcatgat
    27961 tttgaaagag gaagattaga ggagagagaa acatgtcgga gaaaaaagga tttcataatg
    28021 atgacgtggt gttagatatc cacgatcgcc caaccccagg acattgggta ggattaagtt
    28081 tacaacactt gttcacgatg ttcggagcaa ccgtgttagt ccccatctta gtaggtatcg
    28141 atccaggtat tgcgctagtc agttcaggac taggtacatt ggtgtactta tttactacta
    28201 aaggaaagat acctgcgtac ttaggaagca gcttcgcttt cattgcagcg atgcagcttt
    28261 tgatgaaaac agatggttat ccagcgattg cacaaggagc agtcacaact ggattagtct
    28321 atttgattgt tgcgctgatc atcaaaaaga tcggttcagc atggctggat aaaatcttgc
    28381 ctccaatcgt cgtcggtcca gtcatcatgg ttatcgggtt gggacttgcg gcaaatgcag
    28441 caacaaatgc tatgtataat cagggcacgt atgatttcaa gtatattgct gtagcgctga
    28501 tcacattagg attaacaatt ttcttcaaca tgtggctaag aggttttctc ggattgattc
    28561 cgattttact aggtattatt agcggataca tcgtagcctt gctgtttgga atcgtggata
    28621 ctaagccaat cgtagacgct gcctggtttg ctgttcctaa ctttgagatt ccctttgtac
    28681 aatatgaacc aaagctatat cttggagcaa tcacaacaat ggcacccatc gcttttgtga
    28741 cgatgacaga gcatatcggc catcttatgg tattgaataa actaactaaa cgtaatttct
    28801 ttgaagaacc gggtcttcat aagacgttga caggtgatgg attagcacaa atcgtggcag
    28861 cctttgtcgg aggtcctcct gtaacaagtt acggtgaaaa cattggtgtt ctggctatca
    28921 cacgtgtcca tagcgtgttt gtgatcggtg gagctgcagt atttgcagtc attttaggat
    28981 tcgtaggaaa attaagcgct ttgattttaa gtatccctgg accagtcatc tcaggaatca
    29041 gttttatctt atttggagta attgccgcaa gtggaatgaa gattttaatc gagaacaaaa
    29101 tcgattttga taaaaagaaa aatctgttga ttgcttcagt tatcttagta gtaggaattg
    29161 gcggactcgt ctttgaagta ggcacattta cattgtcggc aatggcatta gcaactgtac
    29221 ttggcatcat tttaaatctt atcttaccag aaacttcacg aagcgaagaa aactaaggag
    29281 gaaacattat gatcatcaaa tcagaacgta tcagcttaaa gcatttattg accgttgagg
    29341 cacttactga tcaagaagtt atgggcttga tccgcagagg gcaggaattc aagcaaggtg
    29401 ctaattggac tccgcaaaag cgccagtact ttgctacaaa cctctttttc gaaaatagta
    29461 ctcgcacaca taaaagcttc gatgtagcgg agaaaaaact agggctagaa gtgattgaat
    29521 ttgaagcaag cacaagctct gtccaaaaag gggaaacact atacgataca gtactgacga
    29581 tgtcagcatt aggcgtagac gtagcggtta tccgtcatgg tgatgagaac tattatgacg
    29641 aactgataca aagtaaaaca atccagtgtt cgattatcaa tggaggagac ggaagcggac
    29701 agcatccaac ccaatgcttg cttgatttga tgaccattta tgaagaattt ggtcattttg
    29761 aaggtctgaa agttgccatc gtaggtgata tcactcactc tcgcgtagct aaatccaata
    29821 tgcagatgtt aaaacgtcta ggcgcacaag tattcttctc aggtcctaga gaatggtacg
    29881 acgatgaatt cgaagtgtac ggacattata tgccattgga tgaactacta gatcaagttg
    29941 atgtcatgat gatgcttcgg gtgcaacacg aacgccatga tggaaaagaa agtttctcaa
    30001 aagaaggcta ccatcaggaa catgggttaa cagttgagcg tgcgaagaag ttgcaaaaac
    30061 atgcaatcat tatgcaccca gcaccagtta accgtgatgt agaattagct gattcattag
    30121 tggaaggatt acagtcgcgg atcgttcagc aaatgagcaa tggagtattc gtcagaatgg
    30181 caatcttaga agcagtgcta aatggaaaag cgtaattata ggaggatgaa aaaatgaaaa
    30241 cactcattaa aaacggtcag atcaatacac gaaaaaatat gacgacacca gcagaaattt
    30301 ggatagaaga tgggcgaatc aaagcaatcg gtacaggatt ctcagaagcc gaatttgatg
    30361 aagtgtttga tgcaaaggga caattgatca cacctggttt agtggacgtc catgttcatt
    30421 tgagagagcc aggatttaca tacaaggaga cgatcgaagc tgggactcgc tcagctgctc
    30481 gtggcggttt cactactgta tgcgcgatgc cgaatttaaa tcctgtacct gatacggcag
    30541 aaaagctgag acaagtatat gacatcatta gaaaagatgc agttgtgaag gtattgcagt
    30601 atgcgccaat cacagaaaat ctgcgaagtg agaaattagt tgatcaagaa gcactgattg
    30661 aagaaggtgc ttttgctttc acaaatgacg gtgtaggtgt ccaaacagct gggacgatgt
    30721 atctggctat gaaagaagct gcaaaaaaca ataaagccct agtagcccat acagaagatg
    30781 aatctttatt atttggcggc gttatgcacg ctggcaagaa ggcagaagaa cttggtcttc
    30841 ccgggatctt aagcgtaacc gagtcttctc aaattgctag agatctgttg ctagctgaag
    30901 caactggtgt tcattaccat gtttgccatg tttctactaa agaaagtgtc cgtgtaatca
    30961 gagacgcaaa aaaagcagga atccacgtaa cagcagaagt ttcaccccat catcttattt
    31021 tgatcgacga agatattccc gaagatttcg gcttttggaa aatgaacccg ccgctaagag
    31081 gacgggagga cagagaagct ttgatcgaag gcttgcttga cggtacgatc gattgcatcg
    31141 ctacagacca tgccccccat ggattagaag aaaaaagcca aagtttcatg aaatcaccat
    31201 ttggtatcgt aggaagcgaa acagcgtttc aactgatcta tactcatttt gtagaaactg
    31261 gtcgattcac gttggaacaa gtgatcaatt ggttggcagt taaaccggct gagatttttg
    31321 gtttgaatgc aggaacgcta acggttggtg cccccgctga tgtagcagtt tttgatatta
    31381 cacaaacttg cacgatcgat aaagaagact ttttatcaaa aggggagaat acacctttta
    31441 tcggctggaa agtaaaaggc gaaacacaaa tgacttttgt caacggaaaa cttgtgtggc
    31501 agaagggaga ataaaagtat ggaacgtttg cttatattag aagatggaac agttttcgaa
    31561 ggaaaggctt ttggtgcaga tgctagtgtc ttcggagaaa tcgtttttac aacaagtatg
    31621 actggttatc aagaaactat cactgatcaa agttttaacg gtcaaatcat tacatttacc
    31681 tatcctatgg tgggtaatta tggggtaaat agagatgatt atgaatcgat ttctccaaca
    31741 tgcaaaggag tagtagtaaa agaacatgca cgtgtagctt caaactggcg aaaccaaatg
    31801 acgctagatt catttttgaa acaaaaagga attcctggaa tatcaggtat cgatacgcgc
    31861 gcccttactc gcaaaatccg tgaagtcgga acgatgaaag ctagtatagt tgatgctgga
    31921 gattccttcg aacatgcatt tgatcagctg aaagcaaccg ttctagcaac caatcaagtg
    31981 cagcaagtct caactaacaa accttatcca agtccaggaa caggtcggaa agtcattgtc
    32041 attgacttcg gcttgaaaca tagtatttta cgagaattga gtaaaagaaa ctgtaacctc
    32101 acagtacttc cctataacac agatgccgag acgattttag cgctatcacc agacggagtc
    32161 atgctgacga atggacctgg tgatccaaaa gatgtgccag aagcaatcga aatgatcaaa
    32221 aaaatccaag gaaaagtacc gatttttggt atttgtctcg gtcaccagtt atttgcttta
    32281 gccaacggtg cagataccta taaaatgaaa ttcggacatc gaggattaaa tcacccggta
    32341 cgagaagtag cgaccggaag aattgatttt acttctcaaa atcatggcta tgcagtcgat
    32401 gaagccacga ttgatccaga aaaactcatc gtaactcatg tagaagtaaa tgacggaaca
    32461 gtagaaggag tcaaacacag agattatccc gctttcagtg tgcagtttca tccagatgct
    32521 gcccctggtc cgcatgatgc actacattta tttgatgaat ttatggaaat gatggacgca
    32581 ggaaaggagc agtaaacaga tgccgaaacg tacagatatc aagaaaatta tggtgattgg
    32641 atctggtccg atcatcattg gtcaagccgc agaatttgac tacgcaggaa cacaagcctg
    32701 ccttgcctta aaagaagaag gatatgaagt ggttttagta aattctaatc ctgcgacgat
    32761 catgacggat aaagaagtgg cagatcaagt atatatcgaa ccgatcacac ttgaatttgt
    32821 atcaaggatt cttcgaaaag agcgtccaga tgctctcctt ccaactttag gcggacagac
    32881 aggcctcaat atggcaatgg agctggcagc atcaggtatt ttagaagaat tgaacataga
    32941 acttctagga acaaagcttt ctgcgatcga tcaagcagaa gatcgtgatc tattcaaaca
    33001 gttgatgaaa gaattgaatc agccaatccc cgagtctgaa atcgtcaata cagtagaaga
    33061 agctgtagat tttgcaaata aaatcggcta tcctgtcatc gttcgtccag cctttacttt
    33121 aggcggaact ggtggaggta tgtgtgaaaa tgaagaagaa ttacgtatcg tatcagaaaa
    33181 tggattaagt ctttcccccg ctacccaatg tttgattgaa cgaagtattg caggttttaa
    33241 agaaattgaa tatgaagtga tgagagattc ggctgataat gcaatcgttg tctgcaatat
    33301 ggaaaatttt gacccggtgg gtatccatac aggtgattct atcgtatttg cccctagtca
    33361 aacattgtct gaccatgaat atcaaatgct tcgtgatgct tctctttcga ttattcgagc
    33421 acttaagatc gagggaggct gtaatgtcca actcgctctt gatccacata gttttaatta
    33481 ttatgtgatt gaagtcaatc cgcgtgtttc tcgttcatct gcgttagcaa gtaaggcgac
    33541 tggctatccg atcgctaagc tagctgctaa aatagcgctt ggattaacat tagacgaaat
    33601 gaaaaatcct gttacaggaa cgacttttgc agaatttgaa cctgcattag attatgttgt
    33661 agctaaaatt cctcgctggc cgtttgataa gtttgaaaac ggagaacggc ttttaggcac
    33721 gcagatgaaa gcaactggtg aagtgatggc tatcggtcgg aatatcgaag agtctttatt
    33781 gaaagctgtg cgttctttag aaatcggtac acaccatatc gaacttcctg agttggcaga
    33841 catcacggac gatgacctaa tccaaaaagt ggttcgagca caagatgatc gcctgtttta
    33901 tgtagctgaa gctattagaa gagggtacac catagaagaa ttagcggatt tgacaaaaat
    33961 cgatttattc tttttagata aactgttgca tatcgtagaa atcgaacagc agttgaaaca
    34021 gcagccgttg gatgacaaga tattgaaaac agcgaaacaa aacggtttta cagacaaaaa
    34081 agtggcggag ctgtgggaaa taaacgaaga agccgttcgc cagatgagaa aagaaaatga
    34141 tttgattcct gtatataaga tggttgacac ttgtgcagcg gaattcgaat caaaaacacc
    34201 ttatttttat agttcctatg aatatgaaaa tgaaagttca cgatcagaaa agccctcaat
    34261 tctagtactg ggatctggtc ctatccgtat cggacaaggg gttgaatttg actatgcgac
    34321 tgtgcattct gtcaaagcaa tccagcaggc aggatacgaa gcaatcatca tgaacagcaa
    34381 tcctgaaaca gtttcgactg acttttctat ttcggataaa ttatattttg agccattgac
    34441 cttcgaagat gtgatgaatg tgatcgagct agaacagccg ataggagtaa tcgttcaatt
    34501 tggtggtcag acagcaatca acttagccga acctttgact gctgctggtg taaaaatctt
    34561 agggacaacg atcgaagact tggatcgtgc ggaaaatcgt gatttattcg agcaagcgct
    34621 gaaagagttg gatattcctc aacctttagg ggatactgct acaagtaaag aagaagccgt
    34681 ggttatcgct tcgagaattg gttatccagt tcttgttcgt ccaagctatg tattaggcgg
    34741 acgtgcgatg gaaatcgttg aaaaccagcg agatttagaa gattacatgg aacatgctgt
    34801 gaaagcatct ccagaacatc cagtactagt ggaccgttac ttgcttggtg gtgaatgcga
    34861 agtggatgcg atttgtgacg gtgaaacagt actcatccca gggatcatgg agcatattga
    34921 gcgtgctggt gtccattcag gggattcaat ggctgtgtat ccacctcaaa cattatctga
    34981 atcgatcaag aaaacgatcg aagattacac gatccgctta gcattaggtt tgaattgtgt
    35041 cgggatgatg aatatccagt ttgtcatcca tgaaaatgag gtttatgtga tcgaagtcaa
    35101 tcctcgagca agtcggactg ttccattctt aagtaaggtg acaggaattc caatggcaca
    35161 aattgcgacg aaagctattt tgggagagaa actaaagaca cttggttatg aaaacggact
    35221 ttatccagaa acaaaagaag ttcatgttaa agcacctgtc ttttctttta cgaaattgca
    35281 gaaagttgat acttacttag ggcctgaaat gaaatcgact ggagaagtaa tgggatctga
    35341 cagacaatta gacaaagctt tgtacaaggc attcgaagct tctggattac acttgcctga
    35401 atatggagcc gtattattta cgattgcgga cgaaacaaaa gaagaagcac tagaattagc
    35461 taaacgtttt acagaaattg gctatagtct gataggaacg aaacatacag ctgcttattt
    35521 cgaaaaaaat ggtctggtcg ttgaaacagt agcgaaaatt tctgaggaag cagcagaaaa
    35581 aaatgtagtc gacttaataa gagatggtaa aacacaagtg gttgtcaata cgattgataa
    35641 agatcgggga aatgcttcta aagacggttt catcatccga agagaagcag ttgaacatgg
    35701 tattccattg tttacttctt tagacacagc agatgcgatc gttcgagtga tggaatcccg
    35761 tgctttttca actaacccta tttaacggcg agcacatgtt aagacaaaaa gacgaaaaac
    35821 cgttgcagga ggggacagat gaaacaagaa atgatggaaa tcgttcagca aaaacagctg
    35881 gcaccaaaga tttttgaaat gacattgaaa ggtgatctag taaaagaaat gcagcaacca
    35941 ggacagttct tgcatattct cgtgccgcgt gcagacttgc tattacgccg accgattagc
    36001 ttgaatcata tcgaccacga aacaaatact tgccgtatca tttatcgggt ggaaggtgag
    36061 gggactagag tcttctctac tctttctgcc ggagaccatt tagatgtcat gggtccttta
    36121 ggcaatggtt ttgatttgtc caatcttcat acaggagacg aagtatttat aatcggtgga
    36181 ggaatcggtg tccctcctct atatgagtta tcaaaacagt tagtcgaaaa aggaattaag
    36241 ccaacgcatt ttttaggttt tgccacacat gaagtcgttt attatgaaaa agaatttaat
    36301 cgtctaggag aaacaagaat cgctactgac gatggaacat acggtatgca cggaaatgtc
    36361 ggtaatttat tgttaggaac gaagacggaa cctacagctg tttttgcatg tggttcaaac
    36421 gggttattga agacagtgga acagctgttt tcttcgcatc aaaatgtcca gttatcatta
    36481 gaatcccgca tggcatgtgg gataggggct tgctatgctt gcgtttgtca tacgccggaa
    36541 gatgaaacag gaacaaaaag tgtgaaagtt tgtgacgaag gaccgatttt taaaatagga
    36601 gaggtaatta tatgaatgag aatcctttag cggtcaaact tcctggccta gaattaaaaa
    36661 atcctatcat gccggcaagc ggctgtttcg gttttggcaa agaatacgga aaatactatg
    36721 atttgaatca gcttggatcg atcatggtca aggcaacgac tccaaatgcc cgctttggca
    36781 atcccacgcc aagagtggcg gaaacaccat ctggcatgtt gaatgcaatt ggtttgcaaa
    36841 atcctggtct agacgttgtt atgcataaac ttcttccaga attagaggag tacaatgagt
    36901 tacccatcat cgcaaatgtt gctggtgctt gtgaagaaga ctatgtagaa gtttgtagca
    36961 aaattggcga agcaccaaat gtgaaagcaa tcgagctgaa catttcttgc ccaaatgtca
    37021 aacatggcgg aattgctttt ggaacagatt ctgatgttgc ttttcaatta acacaagcag
    37081 taaagaaagt ttcttctgtt cctgtctatg tcaaattgtc cccaaatgta acagacatcg
    37141 ttccaatcgc tcaggcaatc gaagctggag gggcagatgg gtttacgatg atcaatactc
    37201 ttcttggtat gcggattgac cttaagacaa gaaaaccgat tttagccaat caaacaggtg
    37261 gactctccgg accagcaatc aagcctgtcg ctattcgttt gattcaccaa gtagcaagta
    37321 tatccagcct tccaatcatt gggatgggcg gcgtgcagac agtggatgat gttcttgaga
    37381 tgtttatggc tggagcaagt gctgtagcta tagggaccgc gaatttcacc gatccgtata
    37441 tctgtcctaa actaattaaa gagctccctg tgcgaatgag cgaactaggc atcgaatcgc
    37501 tggaaagatt aatcaaagaa gtgagagagg aacgtgaacg atgaatcaac tagaccaacg
    37561 accaattatt gcattagatt tttcaactag gcaagaggta gaagactttc ttcgattttt
    37621 cccgaaagaa gaaaaattgt ttgtgaaaat cggaatggaa ttgttctacc aagaaggacc
    37681 tgaaatcgta cgttatctaa aagaggctgg tcataatgta tttttagatc tgaaacttca
    37741 cgatattccg aatacggtcg aaaaagcaat gcggggattg gcaaagcttg gtgttgatct
    37801 cacctgtgtt catgccgctg gcggtattcg tatgatggag gcggcaatgc gaggattaga
    37861 agaggggact ccagaaggtg gaaaacgtcc gctacttcta gctatcactc agcttacttc
    37921 tacaagtgaa gaagaaatgc atgccgatca actaatcgaa gtttctttag aaaaaagtgt
    37981 gatccattat gctagctgtg ccaaaaaagc aggattagat ggtgtggttt cttctgcttg
    38041 ggaagtagaa gcgatcaaag aaatggcagg agacgaattt gtttgcttaa ctccgggtat
    38101 aagaccagaa ggaacagtcg caggagatca gacaagagta gtcacacctt ctcaggcaaa
    38161 aaagatcggc tcaactttta tcgttatagg tcgaccaatc actcagtcaa ctaatcctta
    38221 taaagcatat caaacgattc aaacagaatg gagctaacct aaaatgaatg ttgaacaaag
    38281 tatcgcaaaa gacttattag aaattgaagc agtattttta aatccaagta atccttttac
    38341 atgggcaagt ggaatcaaaa gcccaattta ctgtgataac cgaatcacga tgagctatcc
    38401 aaaagtccga aaagagatcg caaaaggatt agcaagcaaa atcaaagaag cttaccctga
    38461 agtacaagtg attgctggaa ctgcaacagc aggaatccct catgccgcat gggtagcgga
    38521 aatactggat ttgccaatgg tctatattcg aagtaaggcg aaagatcatg ggaaaggtaa
    38581 ccaaatcgaa ggacgtatat ttgaaggtca gaagatggtt gtgatcgagg atttgatttc
    38641 tacaggcgga agtgtcttgg aagcagctga agcagcaaaa agagaaggtg cggatatcct
    38701 tggtgttgcg gctatcttta cgtatgagtt gccaaaagga aaagcaaatt tcgaaaaagc
    38761 cgaaatccct cttatgacgt tgaccaatta ttctgtattg attgaagcag ctttagaaga
    38821 tcgttatatc gatgagcaag aattaactct gctaaaagaa tggaaaaaag atcctgaaaa
    38881 ttggcagatt gaagcgcgct tgatgaagat agagaaatcc gatttgtgtc tccagaatca
    38941 tcaattggaa tagcaaaaat ccaaaaactg attgcgagaa gcatcttttc atcaggtatg
    39001 aaaagaaact tctatcgtga tcagtttttg atttttttaa aattcatttt tctaagactg
    39061 gttagcagaa agaagagaat aatctcttct cttttttcta ttatatatag attttctcta
    39121 ttttttggta agggagctgt ttcaaaaatg gtaaagagcg gaaatgttgc gaatatcagt
    39181 tatttaatga tatgatattc ggaaaaagaa atctcaaagg gaggtaactt ttatggggtt
    39241 gaatgaattt attttaggat tgaacaattt agagacgatc acaagggcac ctggattttt
    39301 taaatataca gagcatacag ttgcagcaca ttcgtatcgt gtagcatcta ttgcgcaagt
    39361 tctaggagac attgaagaag cgaatggaca accgatcgat tggaaactgc tctatgaaaa
    39421 atcattgaat catgactaca cagagcgttt tattggtgat atcaaaacgc cggtgaaata
    39481 cgcaagcaaa gagttaagag ggatgctgca aacagttgaa gaaaagatga cggacgaatt
    39541 catcaaccaa gagattccag ctgagttcca agaagtttat cgccgtcggt tgttcgaagg
    39601 aaaagatgaa accgtagaag gagaactatt agccatttca gataaggttg accttcttta
    39661 tgaatctttt gatgaaatca tcaaaaataa cccggaaaaa gtttataagg agatgttcat
    39721 tgaggcagtg actacgatta aaagctttga acatcggcct tctgttcagt atttctttaa
    39781 agagatattt ccggaactat tgaaccagga attttatgga aaagaggcgt ttttagaaac
    39841 cgtctcaacg attgtaaaga aagaagaaaa ataaaaatga tatgagatgc agggggaagt
    39901 atagttgaaa aagaatatgg atgtcgaatg gggctattct ggagacaaag gtccggaaaa
    39961 ttgggcgagt ttatgtgatt ggtttgcgag aggtgcggaa tttccattgc agtcgcctat
    40021 tcatttgacc aagggggaag aaaccaaaat agaaggagac acgctttcct ttcattatca
    40081 aaagcaacgg tttactgaca aggaattcaa aaacacgatt catttagtac cttttgacca
    40141 actcagttat gtagagttca agaaagaacg ctattatttg acagatattc atttccactt
    40201 gccttctgaa catatcatca acggaaaaca ggaagatatt gaatttcatt ttgttcatat
    40261 gaacagcaaa aaagagaatc tagttgtggg tgtcatgtac cagctaatgg tacaggaagg
    40321 ttggctgtat aatcaagaaa atggggaaag ttggaactta gaaaaacatg agcattgggt
    40381 aaatccggat gtgttctttc cggagcaaaa aagccatttc cattatattg gctcattgac
    40441 aactccgcct accagcgggc caatccaatg gtttgtaatg gatcatgtag gaaaactgaa
    40501 ccaagagttt cttctcccct tcgatggtca gtatgctcga ccgaacaacc gaccaatcca
    40561 gcccttgaat ggaagagaaa tttattattt cgaggagttt gagcaatgaa tattcagcag
    40621 atgaaatacg tggtcgctgt agcaaacaat ggtagtttca gagaagcggc aaagaaaatg
    40681 tttgtctccc agccaagtct ttcagctggt atcaaagaat tagaaaccga gttagggatc
    40741 acgctgttta ctcgaacgaa tcgaggggca tatttaacag aagaagggca agacttctta
    40801 aatcgtgccg agaaaatatt ggtccagctt gatacattag aaactcacta tctaacgagt
    40861 gaaaagagag acaatttttc gatcgctgcc cagcattatg atttcttagg tcctttgacc
    40921 gcacagctac tcgaggaatt caaagacgag atcaaacaat ttcggatcat ggagacgaca
    40981 acagcgagag tgatcgaaga agtcaaagag cagcacagtg agatcggcat tatttatatg
    41041 aatgagcata atcaagctgg tatcagaaga tatttacaac aaggagagct aagtggccgc
    41101 agcattggaa aattcaaaac gcatattttt ttaaggcaag gacatccatt agctgaacac
    41161 gcagagatca ccaaagaaga attgttatct tatcctcaag ttcgttttac acaggatgga
    41221 aataatttcc cgtattttta tgaagacctt atcgagatcc ctgatcaaga aacagtgatt
    41281 tatacgagtg atcgaggtac tttgatgaat atcgttcttg aaactgatgc ttacgcttca
    41341 ggatctggta ttgtgattgg agggattcgc gagcacttgc ggttaatccc tttggcagaa
    41401 ggcgaactaa atgaatttta tatcatttat tcggcgaaac gtccgctcag tgaaatcgct
    41461 caaagattta tcaaagagct tactcggtta ctggaccagc agagctaaga aaagcagatg
    41521 agaacgctgt ttattgaaca ccgttgatca aaaaagaggg gttgtgacag aattttgtca
    41581 caactcctct ttttgtagca aacaatttat aactaattgc tgttgtggga aagtacgcaa
    41641 atatgattct agaaacactc attgattacc tgttttgttt catagctgta attttttctt
    41701 cttctggtgt cacgataatc gtcttttggt tttcataaat aacataacca ggcttggcgc
    41761 catttggttt tcggatatgc tttacctgta caagatcaac cggcacttgg gcagagtatc
    41821 ggtattttga aaaataggca gctaactcgg cagcttctgt aattgtttca tctgagggct
    41881 tgtcactttt aatgatgacg tgcgaaccgg gtatattttt tgcatgaagc caatagtcgg
    41941 tttttttagc tgtcttcatt gtcagctggt cattttgaag attgttttta ccaacaagaa
    42001 tgagcgttcc gtcagaagaa aagtattgat caggctgaga tggtttctta cgtttttgtt
    42061 tttttaagtt cttctttttc aggtatcctt ctgaagtcaa ttcttcttta attgcttcga
    42121 tatccattgg gccggcaatt tccagttgag ataagacaga ttcaagatat tggatttcat
    42181 ctttggcttc ttggatttgt tcgccaatca gcttaacagc gttcttcagc ttttggtatc
    42241 gatggaaata tttctgtgca ttttgatttg gtgtcaatgc cggatctagt gctattttga
    42301 ttggccgatc ttcttcataa taattaggta aaacaacttc ttttgcgccg cgtggtactt
    42361 gtgtcagaaa agtagtaagt agttcgccat ttctacggta attctcagcg ttttcggatt
    42421 ctttgagtgt ttgttcacgt tttttcaatt tatttttatt tcgttttagt tcattttcga
    42481 tttttcgaat cagttctccg ccttgttgtt ttgcccgatc tttctctgct ttttcgtgat
    42541 aatagctatc taataattga ctgagtgttg gataagctgt tacagcagat gcctgttcgg
    42601 acaatacctg ataagcaatc ggtgtgaaaa aatctttatt ttccgtttcg taaaaagtag
    42661 gaataggctg atggctgatg gctgagaaaa attcgtccca tacaaccatc ttttcattag
    42721 gacgttccag taatcttttg gtcagctctt gagcgatatc gaaaccgatt ccctgaaact
    42781 gacgttggat tgcttttgga tctagttcag tttgggaaag ccgctgaaag attttttctt
    42841 tctcggagct aaatgggttc aagacctctt gttttggcgg agcaacatat tctacaccag
    42901 gaagcaaaga acgataagtg ttttgagagc ttccaatgtg tttgatcgca tccagtattt
    42961 ttccagtttc tcgattaact aagacgatcg tactatgacg tcccattaat tcaacgatca
    43021 atatgatatt ttgtagatca cccaactcgt ttcgtttagc aaagtggaaa tggatcactc
    43081 gatcattttc gatttgttcg atgtcttcta aaatagcgcc atccaaatat tttcgaagca
    43141 tcatcacgaa gttaggcgga ttatcaggat tctgataatc gatttgggta atctgtacac
    43201 gagcataact tgggtgcgca gataataata atcgatggtt tttgccgcgt gaacgaatca
    43261 ccagcacgat ttcattttca taaggctgat ggattttcga gatccggcca gatagtaacg
    43321 tttcacgcaa ttcgttgacc atcgcgtggg taaatacacc atcaaaggac atggttcatt
    43381 cctcatttct tttcataagt atattcatta taacgaaaaa aagaagaaaa accaatttgg
    43441 ccgaatagta aagcaaaagt ctagaaaaat cttggactaa ttacataaaa aacagtgttt
    43501 ctatcgttga aatccaaatt tttaggt
//
LOCUS       DACDKM010000013        43174 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20834_2016
            SAMN12640370-rid9891663.denovo.013, whole genome shotgun sequence.
ACCESSION   DACDKM010000013 DACDKM010000000
VERSION     DACDKM010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640370
            Sequence Read Archive: SRR10040891
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 43174)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 43174)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742835.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742835.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:04:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,012
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 29 of 75
            Pseudo Genes (incomplete)         :: 33 of 75
            Pseudo Genes (internal stop)      :: 19 of 75
            Pseudo Genes (multiple problems)  :: 5 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..43174
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640370-rid9891663.denovo.013"
                     /isolate="E20834_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            591..1280
                     /locus_tag="IV334_000704"
     CDS             591..1280
                     /locus_tag="IV334_000704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP8942797.1"
                     /translation="MKILIADDDKEIVELLSIYVHNEGYEAVKAYDGKEALSKIRTIP
                     DIELLILDIMMPEMDGMQVVKELRKESQIPIIMLTAKTTDMDKIKGLVAGADDYVTKP
                     FNPLEVMARVKSLLRRTKMQIAPDQPDVLEVNALIINKDSHEVKTIDGKEIQLTALEF
                     GILYLLASHPNRVFSADEIFERVWKQESIVSAKTVMVHVSHLRDKIEEATGGEKVIQT
                     VWGVGYKIDAR"
     gene            1267..2475
                     /locus_tag="IV334_000705"
     CDS             1267..2475
                     /locus_tag="IV334_000705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAP8942798.1"
                     /translation="MTQDSKRATEKRRRINLTSKEISELLAEGIITIILLLLLNVSIL
                     VVISSVINSSPSLTNAIWDSKNIFAERLNTDLFWNGRNFIIPFFFLLDIGVLYWRLIR
                     RYRQMQLRHIISELHYIANGNYDHRIPFELSGDLSRVVTSINGLVDSTVAAIEDERKI
                     EKSKDELITNVSHDIRTPLTSIIGYLGLIEDGQYHSEEDLLKYTHTAYIKAKQMKSLV
                     DDLFEYTKVRQPAVPVNFSAFDMIQLIEQLAADFELEASKKNIQILVQSKVDSLIMDG
                     DTEKLVRVFNNLLTNALKYGKGATKIVIEVERIGSEVVATVKNNGAMIPQQAIDNLFD
                     RFYRVEESRSQATGGTGLGLAIAQSIVALHGGYIYAKSDKQWTSFIMHLPIKKNEKLP
                     IDTQEVIDES"
     gene            2769..4040
                     /gene="serS"
                     /locus_tag="IV334_000706"
     CDS             2769..4040
                     /gene="serS"
                     /locus_tag="IV334_000706"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HAP8942799.1"
                     /translation="MLDVKMMRQNIEEVQEKLMTRGVKKETLAEFMELDENRRKLLVK
                     SEELKKYRNDVSAEIAQLKRAKEDASAKIAEMKEVGGNIKALDTEIAEVDEKLKQIAT
                     TLPNLPHDSVPVGTDEEDNVEIRRWSEPRTFAFEPKPHWEIAENLDILDFERGAKVAG
                     SRFVYYKGLGARLERAVYNFMLDQHIYEHGYTEMITPYIVNSKAMFGTGQFPKFKEDV
                     FQLENTDLTLIPTAEVPLTNYYNDEILDGADLPIYFTALSPAFRSEAGSAGRDTRGLI
                     RLHQFNKVEMVKFSDVEHSYEELEKMTADAEDILKKLHLPYRVMALSTGDMGFSAAKT
                     YDLEVWIPAQDTYREISSCSNCEDFQARRAMIRYRDNDGKIHYTHTLNGSGLAVGRTV
                     AAILENYQNEDGSVTVPEALVPYMGNLKVIK"
     gene            complement(4589..6073)
                     /gene="guaB"
                     /locus_tag="IV334_000707"
     CDS             complement(4589..6073)
                     /gene="guaB"
                     /locus_tag="IV334_000707"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HAP8942800.1"
                     /translation="MSNWETKFTKKGFTFDDVLLIPAESHVLPNEVDMSVQLAKNIKL
                     NIPIISASMDTVTDSKMAIAMARQGGLGVIHKNMTISQQADEVRKVKRSESGVIIDPF
                     FLTPQHLVADAEELMSKYRISGVPIVETLENRKLVGIITNRDMRFVTDYHMPIADVMT
                     KDNLVTAPVGTSLKDAEKILQKHKIEKLPIVDNEGRLSGLITIKDIEKVIEFPNAAKD
                     EHGRLLVAAAVGVTSDTFERANALLEAGADAIIIDTAHGHSAGVIRKIQEIRSTFADA
                     TLIAGNVATAEATKALYDAGVDVVKVGIGPGSICTTRVVAGVGVPQLTAIYDAASVAR
                     QYGKAIIADGGIKYSGDIVKALAAGGHAVMLGSMLAGTDESPGEFEIFQGRRFKTYRG
                     MGSLGAMEKGSKDRYFQGSVNEANKLVPEGIEGRVAYKGSVADIIFQMIGGLRSGMGY
                     VGAANLQQLRDEAQFIQMSGSGLKESHPHDVQITKEAPNYSVES"
     gene            complement(6578..7273)
                     /locus_tag="IV334_000708"
     CDS             complement(6578..7273)
                     /locus_tag="IV334_000708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1129 domain-containing protein"
                     /protein_id="HAP8942801.1"
                     /translation="MEPEQLRAIVKENNSLETKLTKRNQQYIFDLKKSLEAANLSEEE
                     KVKALHDILPTLVAEQKGGRTARQLFGTVSERTEAIINKPAEVKNSSTPLLMWLDNTL
                     LLLGLLSIMIAITGLFSRGTTPAYGFSTLVLGSAIGGWVFYMMYKYIYQYEYPGADRS
                     KKPGMGKTLLILGGTTLLWVVLFSATTLLPPVINPVLDPVVVIIIGATALAIRYYLKK
                     KYGIIGSLSVPRR"
     gene            complement(7363..8463)
                     /gene="ychF"
                     /locus_tag="IV334_000709"
     CDS             complement(7363..8463)
                     /gene="ychF"
                     /locus_tag="IV334_000709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HAP8942802.1"
                     /translation="MALTAGIVGLPNVGKSTLFNAITKAGAEAANYPFATIDPNVGMV
                     EVPDWRLQRLTELVHPKKTVPTTFEFTDIAGIVKGASKGEGLGNQFLSHIRQVDAICH
                     VVRCFDDDNITHVEGRVDPLEDIDTINLELVLADLESINKRYTRVAKVAKTKDKDAVA
                     ELAVLDKIKPVLEEGKSARTIEFTEEEQKIVKSLFLLTTKPILYVANVSEDDVADADA
                     NHYVQEVRNFAAEENAEVIVVSARAEEEIAELDEEDKAEFLEALGIEESGLDQLIRAA
                     YDLLGLATYFTAGEQEVRAWTFKKGIKAPQAAGIIHSDFERGFIRAETVSFEDLDHYG
                     NMHAAKEAGRVRLEGKDYVVQDGDVMLFRFNV"
     gene            complement(8472..8663)
                     /locus_tag="IV334_000710"
     CDS             complement(8472..8663)
                     /locus_tag="IV334_000710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF951 domain-containing protein"
                     /protein_id="HAP8942803.1"
                     /translation="MYDLHDIVEMKKPHACQTNRWEVIRMGADIKIRCMNCGHIVMMT
                     RRDFEKKLKKVIEKNAEKE"
     gene            complement(8684..9574)
                     /locus_tag="IV334_000711"
     CDS             complement(8684..9574)
                     /locus_tag="IV334_000711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HAP8942804.1"
                     /translation="MKKSKGLGRGIDALFQDLENLETVDVKDDTVVQLPLNELRPNPY
                     QPRKTFEEVSLQELANSIEQSGVFQPIIVRRSAVKGYEIIAGERRFRASKLAEKETIP
                     AIVRDFDEEAMMQVAVLENLQREDLNPLEEAEAYEMLMKNLKLTQAEVASRLGKSRPY
                     IANYLRLLSLPDLVKEMVQDQRLSMGQARTLLGLKNKTLILKLANRAVTENLTVRQLE
                     QLVSELNQNDGKEKKKIPRLAKEKPYYIRESEDRLMDKFGTSVAIQEKNGKGKIEIEY
                     LSQKDLTRILDILNIQFDEE"
     gene            complement(9561..10328)
                     /locus_tag="IV334_000712"
     CDS             complement(9561..10328)
                     /locus_tag="IV334_000712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HAP8942805.1"
                     /translation="MAQIISVANQKGGVGKTTTTVNLGACLASLGKKVLLVDMDAQGN
                     ATSGVGIRKPDVTRDIYDVLVNELPIDEATLITEHENLSIVPATLQLAGAEIELTSMM
                     ARESRLKGSLAEVSSQYDYILIDCPPSLGHLTINSFTASDSILIPVQCEYYALEGLSQ
                     LLNTVRLVQKHFNPELEIEGVLLTMYDARTNLGNEVVEEVRKYFREKVYETIIPRNIR
                     LSEAPSHGKPIIDYDPRSRGAEVYQALAKEVVSREEK"
     gene            complement(10340..11053)
                     /gene="rsmG"
                     /locus_tag="IV334_000713"
     CDS             complement(10340..11053)
                     /gene="rsmG"
                     /locus_tag="IV334_000713"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HAP8942806.1"
                     /translation="MIPEEFRQSLAEKGIELTDHQMNQFAIYYRTLVEWNEKMNLTAI
                     TDEKDVYLKHFYDSLTLAFEVDFTQPLSLCDVGSGAGFPSIPLKIAFPELQITIVDSL
                     NKRITFLSELTRLLGIDASLYHDRAETFGQNPHFRASFDLVTARAVARLNVLAELCLP
                     LAKQEGYFYALKAAKSEEELTEAKQAIALLGGKFLKEEAVTLPNVSDQRHIIVIQKKK
                     PTPKKYPRKPGLPNKQPIK"
     gene            complement(11285..13186)
                     /gene="mnmG"
                     /locus_tag="IV334_000714"
     CDS             complement(11285..13186)
                     /gene="mnmG"
                     /locus_tag="IV334_000714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HAP8942807.1"
                     /translation="MEHYEADAYDVIVVGAGHAGSEAALAAARMGSKTLLLTINLDMV
                     AFMPCNPSVGGPAKGVVVREIDALGGEMGKNIDKTYIQMRMLNTGKGPAVRALRAQAD
                     KHAYSTEMKHTIEKEPNLTLRQGIVEELIVEDGVCHGVVTSTGASYRAKAVVITAGTA
                     LRGEIIIGELKYSSGPNNSQPSIGLANHLKELGFAIERFKTGTPPRVKSSTIDYSVTE
                     EQPGDKEPNHFSFSTPDSAYKLDQESCWLTYTGETTHQIIRDNLHRAPMFTGIVEGVG
                     ARYCPSIEDKIVRFADKPRHQLFLEPEGLNTEEVYVQGLSTSLPEDVQVNMLHSIAGL
                     EKVEMMRTGYAIEYDVVEPHQLRPTLETKVVENLYTAGQTNGTSGYEEAAGQGLIAGI
                     NAALKVQGKEPLVIKRSDGYIGVMIDDLVTKGTNEPYRLLTSRAEYRLLLRHDNADLR
                     LTEMGHRVGLVKEDQYKNYLKKKEAVEQEIKRLMNVRIKPTEEVQDFLATINAAPLKD
                     GVLASDFLRRPEITYAELLRFIPENEELTRKEIEQVEIQIKYEGYIKKAMEKVEKLKR
                     MEAKKIPENIDYAAINGLATEAKQKLQKIQPETIAQASRISGVNPADLSILMVYIEQG
                     KIAKVSEPA"
     gene            complement(13203..14600)
                     /gene="mnmE"
                     /locus_tag="IV334_000715"
     CDS             complement(13203..14600)
                     /gene="mnmE"
                     /locus_tag="IV334_000715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HAP8942808.1"
                     /translation="MAEITLEFDTIAAISTPPGEGAISIVRLSGDQAVQLADKVYQSG
                     NKRLSEVPSHTIHYGHIVDPKSNQLVDEVMVSVMRAPKTFTREDVVEINCHGGIVVVN
                     QILQLLLREGARLAEPGEFTKRAFLNGRVDLSQAEAVMDLIRAKTDKAMGLALNQLDG
                     NLSALIRSLRQEILETLAQVEVNIDYPEYDDVEELTTKLLLEKAQMIQQRIQALLATS
                     KQGKVLREGLSTAIIGRPNVGKSSLLNHLLREEKAIVTDIAGTTRDVIEEYVNVRGVP
                     LKLIDTAGIRETEDVVERIGVERSRKALAEADLILLVLNQSEPLTAEDEQLLEATSGL
                     KRIILLNKTDLPAQLEQEKLKKLIENEPVFSISVAKNDGLDRLESAISDLFFSGETGE
                     RDATYVSNTRHIALLEKASLSLEEVIAGIDSGMPVDLVQIDMTRCWDYLGEVVGDSVQ
                     DELITQLFSQFCLGK"
     gene            14944..15282
                     /locus_tag="IV334_000716"
     CDS             14944..15282
                     /locus_tag="IV334_000716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8942809.1"
                     /translation="MSPFRSAALEEESDPRINDVVLSTGNINQKYIVRDIVFVVECLE
                     ADLFDPSFPKDDLFLTTKRKLKTKVLEYGANAVINCHFEYQQKKRDGKYFLELFAYGT
                     VIQFIQTTIG"
     gene            complement(15450..16208)
                     /locus_tag="IV334_000717"
     CDS             complement(15450..16208)
                     /locus_tag="IV334_000717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HAP8942810.1"
                     /translation="MPIYEAATIDEAVQLGLADLGLAKDQVEIEILEEGKKGFLGMGR
                     KNARLSIQPSISEKVSETVEQTVEEILEEEPTAEIEVVNETILEEKTEEVSELGDEAA
                     LTELAVYLTEISKQLDAPALVKMERESNNMVVFHLETQKQGILIGKHGKTLNALQYLA
                     QVFIHRLARNKLSVVVNVGNYREKRQEIIQRLADRTAEKVKRTGQPVFLEPMPAFERK
                     QIHAALSKDEHVKTHSEGEEPYRYLVVEPAKKYY"
     gene            complement(16226..17044)
                     /locus_tag="IV334_000718"
     CDS             complement(16226..17044)
                     /locus_tag="IV334_000718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HAP8942811.1"
                     /translation="MKKYKRLLLMAGIVSLVIVLSGCGTGEVSAQSTGIWDRYIVYYF
                     AEAIKALSFGNAGIGIILFTLIIRVILLPLMHFQTKSMRKTQELQPKLKALQQQYSSR
                     DPETQSKLREEQQRLYAENGVNPYAGCLPLLVQMPILMALWQSISRVPELKQGHFLWL
                     NLGNADPTFILPILAAIFTFASTYLSSMSQIESNASMKIMNFVMPVMILVMGVNLASG
                     LSLYWVVSNAFQVVQTLLINNPFKIRKEREEEARRIRERERALKKAMNPKKKRK"
     gene            complement(17041..17397)
                     /gene="rnpA"
                     /locus_tag="IV334_000719"
     CDS             complement(17041..17397)
                     /gene="rnpA"
                     /locus_tag="IV334_000719"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HAP8942812.1"
                     /translation="MRKAYRVKKEREFQAVFHTGDSFANRKFVVYRLEKPGQPHFRVG
                     LSVGKKVGNAVNRNEVKRKIRAAVYSIKEEIDPELDFIVIARPSVKGLTYDEVRSNLV
                     HVLKLAKIIDREEKSK"
     gene            complement(17515..17649)
                     /gene="rpmH"
                     /locus_tag="IV334_000720"
     CDS             complement(17515..17649)
                     /gene="rpmH"
                     /locus_tag="IV334_000720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003397591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HAP8942813.1"
                     /translation="MKRTYQPNKRKRQKVHGFRKRMSTKNGRRVLASRRRKGRKVLSA
                     "
     gene            18172..19506
                     /gene="dnaA"
                     /locus_tag="IV334_000721"
     CDS             18172..19506
                     /gene="dnaA"
                     /locus_tag="IV334_000721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HAP8942814.1"
                     /translation="MVSLDALWNELKATYQKDLSPASYNTWIETANPRTLDQNQLVVE
                     VPSKIHKEYWEKNLATKIVEVGYMLSGNEIIPRFITGEEAEQEEVIEEKNPKVVAPSP
                     LKKAMLNPKYTFDTFVIGKGNQMAHAAALVVAEDPGSIYNPLFFYGGVGLGKTHLMHA
                     IGHQMLQSQPNAKVKYVSSETFANDFINSIQNKTAEEFRQEYRNVDLLLVDDIQFFAE
                     KEATQEEFFHTFNALYNEGKQIVLTSDRLPNEIPKLQERLVSRFAWGLSVDITPPDLE
                     TRTAILRKKAAAERLEIPDDTLSYIAGQIDSNIRELEGALVRVQAFAAMNSEDISTSL
                     AADALKTLKSGKGHPQLSILQIQEEVAKYYHIQLKDLKGKKRVKSIVVPRQIAMYLAR
                     ELTDNSLPKIGAEFGGKDHTTVIHAHEKIQQLMDSSVSMQNEVSEIKNFLLN"
     gene            19704..20834
                     /gene="dnaN"
                     /locus_tag="IV334_000722"
     CDS             19704..20834
                     /gene="dnaN"
                     /locus_tag="IV334_000722"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HAP8942815.1"
                     /translation="MKVTLNRASFMQELQTVQRAISSKTTIPILTGVKITLTQEGLTL
                     TGSNADISIETFLSVENEKANMQIESTGSIVLQARFFSEIIRRLPEETFTLEVLENKQ
                     VAITSGKANFIVNGLDADNYPHLPVVESHNQMKLPVHVLTKLINETVFAVSQHESRPI
                     LTGVHFILSDNSLLAVATDSHRLSQRVIPVEQAADHFDIVIPGKSLIELSRSLTNEEE
                     IVEISIMENQVLFKTETMYFYSRLLEGNYPDTNRLIPSSFNTEVEFSVPSFLAAIERA
                     SLLSHEGRNNIVRLSIRPDAVVLYGNSPEIGKVEESLSYTASSGDPLDISFNPDYMKA
                     ALRAFGDMSIKVKFISAIRPFTLEPTEDGVQFIQLITPVRTN"
     gene            21063..21305
                     /gene="yaaA"
                     /locus_tag="IV334_000723"
     CDS             21063..21305
                     /gene="yaaA"
                     /locus_tag="IV334_000723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S4 domain-containing protein YaaA"
                     /protein_id="HAP8942816.1"
                     /translation="MKQQVILNTEFMTLGQMLKEVTVIGSGGQAKWYLAENTVLVDGE
                     PENRRGRKLYPGMMVEVPEVGTFFMVKKGNDDNEAE"
     gene            21292..22416
                     /gene="recF"
                     /locus_tag="IV334_000724"
     CDS             21292..22416
                     /gene="recF"
                     /locus_tag="IV334_000724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HAP8942817.1"
                     /translation="MRLNKLYLKNYRNYEELNIEFSKNLVIFLGENAQGKTNLLESIY
                     VLAMTRSHRTSSEQELIGWTDDQAMIQGEITKGSSTLPLEILLSKKGRKTKINHIEQK
                     KLSTYVGQMNVILFAPEDLSLVKGSPQTRRKFLDMELGQINPIYLYDLVQYQAILKQR
                     NQYLKQLNEKKQTDLLYLDILSEQLAAFGSKVLKARAQFVKRLEFWANSLHQQITHQK
                     EQLEIEYLTAVDSLETHTQEQIQEQFLALLNQNKKKDLFRGTTTVGPHRDDLSFFINQ
                     KNVQTYGSQGQQRTTALSVKLAEIDLIKEETGEYPILLLDDVMSELDDNRQLHLLETI
                     EGKVQTFLTTTTLDHVKNKMTVEPEIFYVQQGKLKGEETE"
     gene            22413..24359
                     /gene="gyrB"
                     /locus_tag="IV334_000725"
     CDS             22413..24359
                     /gene="gyrB"
                     /locus_tag="IV334_000725"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003129249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HAP8942818.1"
                     /translation="MTEERSLVERAKEYDASQIQVLEGLEAVRKRPGMYIGSTSSEGL
                     HHLVWEIVDNSIDEVLAGFATKIHVIIEKDNSITVIDDGRGIPVDIQAKTGRPAVETV
                     FTVLHAGGKFGGGGYKVSGGLHGVGSSVVNALSTQLDVKVYKEGNVYYQEYRRGAVVD
                     DLKIIEQTDRHGTTVHFTPDPEIFTETTEFDFSKLATRIRELAFLNRGMRISIEDKRE
                     EEPVINEYHYDGGIKSYVEYLNANKTVIFPEPVYLEGEQQDIAVEVSMQYTDGYHSNI
                     MSFANNIHTYEGGTHESGFKTALTRVINDYARKQKLMKENDDNLTGEDVREGLTAVIS
                     IKHPDPQFEGQTKTKLGNSEVRTVTDRLFSEHFMKFLLENPSVGRQIVEKGLLASRAR
                     LAAKRAREVTRRKGALEISNLPGKLADCSSNDPEKCELFIVEGDSAGGSAKQGRNREF
                     QAILPIRGKILNVEKASMDKILANEEIRSLFTAMGTGFGEDFDVSKARYHKLVIMTDA
                     DVDGAHIRTLLLTLFYRYMRPIVEAGYVYIAQPPLYGVKQGKNITYVQPGKNAEEELK
                     QVVASLPASPKPSVQRYKGLGEMDDHQLWETTMDPSNRMMARVSVDDAIAADQIFEML
                     MGDRVEPRRAFIEENAHYVKNLDI"
     gene            24379..26850
                     /gene="gyrA"
                     /locus_tag="IV334_000726"
     CDS             24379..26850
                     /gene="gyrA"
                     /locus_tag="IV334_000726"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HAP8942819.1"
                     /translation="MSEEIRENIHDVNLTSEMKDSFIDYAMSVIVARALPDVRDGLKP
                     VHRRILYGMNELGVTPDKAHKKSARIVGDVMGKYHPHGDYAIYESMVRMAQPFSYRYM
                     LVDGHGNFGSVDGDGAAAMRYTEARMSKIATEMLRDINKNTVDFQSNYDDTEKEPVVL
                     PARFPNLLVNGTTGIAVGMATNIPPHNLSEVVAAIDLLMDNPDVTTNELMEVLPGPDF
                     PTGGLVMGKSGIRRAYETGKGSITVRAKVEITEMPNGKERIIVTELPYMVNKAKLIER
                     ISELHRDKRIEGITDLRDESSREGMRIVIDIRRDASASVILNNLYKLTSLQNSFGFNM
                     LAIEKGVPKVLSLKQILENYIEHQREVITRRTVFEKEKAEARAHILEGLRIALDHIDE
                     IIAIIRGSKSDDEAKATMIERFDLSDRQSQAILDMRLRRLTGLEREKIENEYQELLKL
                     IEDLTDILARPERVTEIIKNELADLNQRFGDKRRTELLVGEVLSLEDEDLIEEEEIVI
                     TLTNNGYIKRLANNEFRAQRRGGRGVQGMGIHNDDFVKNLVSCSTHDTLLFFTNNGKV
                     YRAKGYEIPEYGRTAKGIPIINLLGIDSSESIQAIIAVTGEAEEGHYLFFTTRQGTVK
                     RTSVTAFANIRSNGLIAIGLKEDDELVNVLLTDGQSNIIIGTHNGYSVTFKEEAVRDM
                     GRTASGVRGIRLREEDYVVGAALMKEDQEVLVITEKGYGKRTKVSEYPVKGRGGKGIK
                     TANITEKNGPLAGLTTVSGDEDIMLITDKGVIIRFNVSTVSQTGRSTLGVRLMKMEAD
                     TKVVTMAAVEPEAAEQTVEEQGSEE"
     gene            27086..27385
                     /locus_tag="IV334_000727"
     CDS             27086..27385
                     /locus_tag="IV334_000727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HAP8942820.1"
                     /translation="MENTKYEIMYIIRPNIDEEAKTALIERFDAILKDNGAEVIESKD
                     WEKRRFAYEMNGYREGIYHIVKVSSPSSANAVNEFDRLAKINDDIIRHMIVKEEA"
     gene            27435..27947
                     /gene="ssb"
                     /locus_tag="IV334_000728"
     CDS             27435..27947
                     /gene="ssb"
                     /locus_tag="IV334_000728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016186236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HAP8942821.1"
                     /translation="MINNVVLVGRLTKDPDLRYTASGTAVATFTLAVNRNFTNQSGNR
                     EADFINCVIWRKSAETLANYARKGTLLGVTGRIQTRSYDNQQGQRVYVTEVVADNFQL
                     LESRSASENRQQSGGFQSSGQSAGGFGGNNNSNQTSQSSNGMPDFDRDTSDPFGSSST
                     IDISDDDLPF"
     gene            27973..28209
                     /gene="rpsR"
                     /locus_tag="IV334_000729"
     CDS             27973..28209
                     /gene="rpsR"
                     /locus_tag="IV334_000729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003129253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HAP8942822.1"
                     /translation="MAQQRRGGRKRRKVDYIAANHIEYIDYKDIELLKRFISERGKIL
                     PRRVTGTGAKNQRKITVAIKRARIMGLLPFVSED"
     gene            28352..30325
                     /locus_tag="IV334_000730"
     CDS             28352..30325
                     /locus_tag="IV334_000730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HAP8942823.1"
                     /translation="MENKNNHLSPKPLFLILFILIEVLVVLILPFRFVSASVILLMNL
                     WGVRYWLKMKRWLILNKREKIQQASTVAETNLAYVSEVMPVGVIAYQPSSQKIEWLNP
                     LALKIKDQSGLTEEELLDTFFTAKEKKKNQVYLADKTFQFDMDTVKGVVYFNDITQNQ
                     LLKLHQTNSQPVIGILSIDNYADAIDKLDDKEISYLNSFVTTLVSDWMNEYHVFFKRI
                     NAERFFFVAQMIDLKKMMDKKFDLLDRFRAEAEKQSSPITLSMGIAYGSRTLDEIGGE
                     AQNNLDVALVRGGDQVVVKEVKEGAKPVYYGGKSAKNVKRTRVRSRAMSTALRRIFDE
                     TGDVFIMGHKFPDMDALGSAFGVSCLARFNQKKAYIVINENEIIPDVERCLEEIHKHP
                     ELEMQLISPERAIELKKEEDLLVMVDYHKPSLSISQGLYEQFDKIVIIDHHRRGEEFP
                     SKPLLTYIESSASSASELVSELIQYQSSKTNRLDRFEATLLLAGMVVDTNSFNVRTTA
                     RTFDVASYLRSCGADASLVQYLLSSDLATYLEISNLIAGSDYVTSDIVVAAGTDEKEY
                     NSVIAAKTADTLLSMININAAFVITKRTDGLIGISARSTGTINVQIIMESLGGGGHFT
                     NAATQLENTTVEETKQKLFAAIKENMNQIYDKE"
     gene            30331..30783
                     /gene="rplI"
                     /locus_tag="IV334_000731"
     CDS             30331..30783
                     /gene="rplI"
                     /locus_tag="IV334_000731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HAP8942824.1"
                     /translation="MKVIFLQDVKGKGKKGDIKEVPAGYAQNYLIKNNLAKEATKSSI
                     SQLAGQKKAQEKHEAEILEEAKKLKEFLEKEDTVVELKAKAGEDSRLFGSIPSKQIAE
                     GLNKQYKIKLDKRKIELENPIRSLGYTNVPVKLHHEVTATIKVHVVQE"
     gene            31057..32424
                     /gene="dnaB"
                     /locus_tag="IV334_000732"
     CDS             31057..32424
                     /gene="dnaB"
                     /locus_tag="IV334_000732"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HAP8942825.1"
                     /translation="MNEVWQDRIPPQNIEAEQAVLGSVFLDAETIIDAMEYIEPKDFY
                     RRGHQIIFETMIELNDRNEAIDVVTVKDRLEQANLLEDAGGLSYLSDLALAVPTAANI
                     VYYAKIVEEKSLLRTLIQTATGIVTKGFEQGEEVQTILDDAERSILEVSEKRNRSGFL
                     SIAEVLNSTIENIDRLAQNNEEITGLPTGYQGLDKMTAGFQPEELIILAARPAVGKTA
                     FALNIAQNVGTKTDRSVAIFSLEMGAESLVNRMLCAEGSIEASHLRTGQLSEEEWSNL
                     IVAMGSLSRANIFIDDTPGIKISEIRAKCRKLAQEKGNLGLILIDYLQLIEGNGKENR
                     QQEVSDISRQLKKLAKELKVPVIALSQLSRGVEQRQDKRPVLSDIRESGSIEQDADIV
                     AFLYRDDYYEREGGDDDDGNEPKNDNVIEVIIEKNRSGARGTVELLFIKEYNKFSSLS
                     PREEF"
     gene            33204..34496
                     /locus_tag="IV334_000733"
     CDS             33204..34496
                     /locus_tag="IV334_000733"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HAP8942826.1"
                     /translation="MSSVVVVGTQWGDEGKGKITDFLSENAEVIARYQGGDNAGHTIK
                     FNGVTYKLHLIPSGIFYKDKISVIGNGVVVNPKSLVKELAYLHEKGVETSNLRISDRA
                     HVILPYHIKLDQLQEDAKGENKIGTTIKGIGPAYMDKAARVGIRIADLLDKEIFEERL
                     RLNLDDKNRQFVKMFDSEPLDFDDIFEEYYEYGQQIKQYVTDTSVILNDALDAGKRVL
                     FEGAQGVMLDIDQGTYPFVTSSNPVAGGVTIGSGVGPSKINKVVGVCKAYTSRVGDGP
                     FPTELFDETGEQIREVGREYGTTTGRPRRVGWFDSVVMRHSKRVSGITNLSLNSIDVL
                     SGLETVKICTAYELDGELIYHYPASLKELNRCKPIYEELPGWSEDITGCKTLAELPEN
                     ARNYVRRISELVGVRISTFSVGPDRTQTNILESVWAQI"
     gene            35232..36095
                     /locus_tag="IV334_000734"
     CDS             35232..36095
                     /locus_tag="IV334_000734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HAP8942827.1"
                     /translation="MYQIVTDSCCDLPYHVLAKEKVDFLSMNLQLNDQELVDDLGKTF
                     NYDRYMNQLKNGAMPTTSQINVGRYLEFFRSYAEKQIPVLYVAFSSGLSGSYSSALQA
                     VELLKEEYEDPEIYVFDTKAASIGEGLLVLEAVRLKKEGKSIEETLEWLESNYLKVHS
                     WVTVDDLKYLERGGRISKASATLGSLLSVKPIITVDKEGRLTNVDKVRGRNKSIQTIV
                     NETVKTIVAPLDQIVYVAYAGDLESAEKAKRLLEEQIKMKDVKLYPLGPTIASHTGYG
                     CIAIFSMGISR"
     gene            complement(36224..37846)
                     /locus_tag="IV334_000735"
     CDS             complement(36224..37846)
                     /locus_tag="IV334_000735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAP8942828.1"
                     /translation="MITVNDVSLHFSDRKLFDDVNIKFTPGNCYGLIGANGAGKSTFL
                     KVLSGDLQPSTGSVTMGPDERMAVLKQNHYDYEDYTVLETVIMGHKRLYEVLKEKDAI
                     YMKEDFTDEDGIRAAELEGEFAELNGWEAEPEAAVLLQGLNIPEELHHQQMSELTGGQ
                     KVKVLLAQTLFGKPDVLLLDEPTNGLDIQSINWLEEFLINFENTVIVVSHDRHFLNKV
                     CTHMADLDFGKIKLYVGNYDFWLESSQLAAKLQANANAKKEEQIKELQEFIARFSANA
                     SKSKQATSRKKMLEKITLDDIQPSSRRYPFVGFKPEREIGNDLLQVENVSVTIDGKKI
                     LDDISFNLSKEDKVAFVAENDITTTTLFKVIMGEITPDTGTVRWGVTTSNSYLPKDTT
                     KEFDTDLTILDWLRQYASKEEDDNTFLRSFLGRMLFSGEEVLKPVNVLSGGEKVRCML
                     SKMMLSKANVLVLDDPTNHLDLESITALNDGLMAFTGSILFASHDHQFIQTLANRIIA
                     VSDKGVIDRADTTYDEFLENADIQKQLNELWA"
     gene            38095..38616
                     /locus_tag="IV334_000736"
     CDS             38095..38616
                     /locus_tag="IV334_000736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8942829.1"
                     /translation="MDEKRTIDWGSLILGILFVLVSLLSFQDPVGNLVAIVVVFAIFA
                     FIKGIFELFVRNRLKELTGYKGKMPLIIGIIDILVGVFFLFNIGAGVAALPFVFAVWF
                     IADSVLALFTADLARGVSEGYYWFTIIVNILGILLGIFLLFNPISSALTLSFLVGFYF
                     MLFGITHIVYAFR"
     gene            complement(38802..40532)
                     /gene="spxB"
                     /locus_tag="IV334_000737"
     CDS             complement(38802..40532)
                     /gene="spxB"
                     /locus_tag="IV334_000737"
                     /EC_number="1.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate oxidase"
                     /protein_id="HAP8942830.1"
                     /translation="MSKINAGIAMVKVLESWGIDHIYGIPGGSFNSIMDALYQEKTEI
                     NYIQVRHEEVGALAAAADAKLTGKVGAVFGSAGPGATHLINGLYDAQMDHVPLVAFLG
                     QVASTSMNYNSFQELNENPIFADVSVYNRTVMTPQSLPHVVDEAIKAAYEHKGVAIVT
                     IPVDLGFEEIDEQPFSTAHTHKTSVILPEEKDLLAALPYLEKAKKPVLYVGQGTRNGF
                     PQIKEFSEHFSVPVVASVLAKGIIPDDYENFLGFAGRVATKPGNEALAEADLILFVGS
                     DFPFGRAFFNPTAEFIQIDIDASKFGRRHETSLSILGDANTALQRLVELGDARPADSW
                     YLANQENKEKWVNWLKSFEDREEEPIRPEAVYKEINRIAEDDAIFVTDVGNTTIHSIR
                     LLNMNGKQKHTTSGWFATMGNGVPGGIAAQLSYPEKQVFTLSGDGGFAMVMQDIITQV
                     KYQLPIINVVFSNDSFGFIEAEQEDTEQKKFGVFLEDADFGKVGEALGANSFTVTEYS
                     QLRFAFDAAAKSDRPVVIDVKINNKRPLPVEDLHLDPRKYSEEEIQAFKEKYEVHDLP
                     VLNELYEKRL"
     gene            40697..41515
                     /locus_tag="IV334_000738"
     CDS             40697..41515
                     /locus_tag="IV334_000738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005232914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HAP8942831.1"
                     /translation="MVEWLLRLEPWQQALAGTAFTYFMTALGAGLVFFFKEIKKEVLN
                     LMLGFASGVMIAASFWSLLDPAITKAEENGDIAWLVVSIGFGLGGVFLYMADKTLPHM
                     HFGPQHEAEGLPTHLKRTILLVFSITLHNIPEGLAVGVAFGAAATADNPTAAILAAVS
                     VALGIGIQNFPEGAAVSIPLRQEGLSRKKAFVYGQASGIVEPIAGVIGALLVTKVELL
                     LPYALAFAAGAMIYVVVEELIPEAQQTLSSKRHFAVFGVMSGFIIMMILDVALG"
     gene            complement(41618..41815)
                     /locus_tag="IV334_000739"
     CDS             complement(41618..41815)
                     /locus_tag="IV334_000739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8942832.1"
                     /translation="MRQKNMFLNIFMVLLVLIAASFVISLFVSVLSGLLWFAVKVLIP
                     IALIIWLVRWIKGNTHRKNYY"
     gene            41984..42562
                     /locus_tag="IV334_000740"
     CDS             41984..42562
                     /locus_tag="IV334_000740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1211 domain-containing protein"
                     /protein_id="HAP8942833.1"
                     /translation="MPKNRLEAFTDAVIAIIMTILVLELHEPNGDTWEALAALGYRFF
                     IYIVSFIILAIYWNNHHHLFQVVHKINGRVLWANSFFVFALSLFPFATSWISEYINSL
                     APEMTYGFVTLTANIAYYILAKELVKANKGHEKALKAMLNNYKKSYLSIGLNVLGIVL
                     GYLVAPVAVLIANIVILAAWIIPSRTIESQYR"
BASE COUNT        13131 a         8266 c         8514 g        13263 t
ORIGIN      
        1 ggagtgtttc ccgattcgtt tttggctgcc gtagcaactt ttatatcata tcagagagac
       61 aagtaattgt caactctttt ttaaaagttt tttcgatttc gtttttcgaa atcttccagc
      121 aactcattta tcataactta tcgtttgtta tttgtcaaga acttttttga agtttttttc
      181 gatagaatca agcttcgcaa gtggttcttt tttaacgact cagttatctt atcatgtcat
      241 ttccaacatg tcaacaactt attttttatt atttaataag ttattggtgt tcttttgaac
      301 aaataataat atatcaacat cacatgtgaa agtcaataag aaaatgctta tttatcaaca
      361 tttctagctt acatgatcat tggttagttc tggtcctttt tcgttcgtct ttataactcc
      421 gagagacgta aaatcctttt gttttcagtt taatgttcat gttcttgtct tattcacacc
      481 aactagatgt ctcaccttct aaaattttcc ttaacaaagt gctgctttta tctatttctg
      541 cttttttttg ctagaataat agtaacaata tgactctgga ggatttattt atgaaaattt
      601 tgattgctga cgatgataaa gaaatcgttg aattattaag tatctatgtt cacaatgagg
      661 ggtatgaagc agtaaaggct tatgatggga aagaagcatt atctaaaatc agaacaatcc
      721 ctgatattga attacttatt ttagatatca tgatgcctga gatggatggg atgcaagtag
      781 tgaaagagct acgaaaagaa tcccaaatac caatcattat gctgacagca aaaactactg
      841 atatggataa gatcaaagga ctggtcgctg gtgcagatga ttatgtgacc aagccattta
      901 atccgttaga agtaatggct agagtaaagt ctttattacg gcgtacaaaa atgcagattg
      961 ccccagatca gcctgatgta ttagaagtaa atgcattaat catcaataaa gactctcatg
     1021 aagttaaaac aatcgatggg aaagaaatcc agttaacagc tttggaattc ggtatcctct
     1081 acttgcttgc tagtcatcct aatcgagtat ttagcgcgga tgaaattttt gagcgtgttt
     1141 ggaaacaaga aagtattgtc tctgctaaaa ctgtcatggt tcacgttagt catttaagag
     1201 ataagattga agaagcaact ggtggtgaaa aagtcattca aaccgtttgg ggagtgggct
     1261 ataaaattga cgcaagatag taaacgagca actgaaaaac gccgtcggat caatttgact
     1321 tcaaaagaaa tcagcgaatt gttagcggaa ggaatcatca cgattatcct tttgcttcta
     1381 ttaaatgttt ctattctggt tgttatcagt tcggtgatca acagttcacc ttctttgacg
     1441 aatgcgattt gggattcaaa gaatattttt gcagaacgat tgaacactga tttgttctgg
     1501 aacggccgga attttattat tccctttttc tttctattag atattggtgt tttgtattgg
     1561 cgcttgattc gaagatatcg ccaaatgcaa ttgcggcata tcatcagcga acttcactac
     1621 atcgcaaacg gaaattatga tcatcgtatc ccttttgaac tgagtggtga cttaagccga
     1681 gtggtaacaa gtatcaatgg attggtggat agtaccgtag cagcgattga agacgaacgt
     1741 aaaatcgaaa aatcaaaaga tgaattgatc acgaatgtca gtcatgatat ccgtacccca
     1801 ctcacgtcca tcattggata cctaggactg atagaagatg gccagtatca ctcagaagaa
     1861 gatcttttaa agtatacgca tactgcttat atcaaagcaa agcagatgaa atctttagtg
     1921 gacgacttat ttgaatacac taaggtacgc cagcctgctg tcccggtgaa tttttctgcc
     1981 tttgacatga tccagttgat cgaacagtta gcagctgatt ttgagttgga agcttctaaa
     2041 aaaaatatcc aaatacttgt tcaatcaaaa gtagactccc ttatcatgga tggcgatacc
     2101 gaaaaacttg tacgtgtctt caataatctt ttgacgaatg cgttgaaata cggaaaagga
     2161 gcaacgaaga tcgtaatcga agtagaacgt atcggttcag aagttgttgc aacagtcaaa
     2221 aataacggag caatgatccc acaacaggca atcgataatc tattcgaccg tttttatcgt
     2281 gtagaagaat ctcgttcaca agcaactgga ggaactggac ttggtttagc aatcgctcaa
     2341 agcattgtgg ctttgcacgg cggatacatt tatgcaaaat cagataagca atggacttct
     2401 ttcatcatgc accttccaat caagaaaaat gaaaaattac ctattgatac acaggaagtt
     2461 attgacgaaa gctgaaatat tcgctattct tattacaatt attagaataa atgaaaaaca
     2521 cagaacccag tacactttat tctttttcag agagctgatg gcaggtgtaa atcagcaaag
     2581 gataatttgg aagttccagt tcgtttacgt gccctttcga aataagaaag gtcggttcaa
     2641 cagccgttat gtgttcaaca agtgcagctg acgctgaact tgggtggtac cgcgaatttc
     2701 agactgattc gtcccaagaa taatgggatg gatcagtctt tttttatttt caaaaaggag
     2761 gaaatcatat gttagatgta aaaatgatgc gacaaaatat tgaagaagta caagaaaaat
     2821 tgatgactcg gggcgtaaaa aaagaaacat tagctgaatt catggaacta gatgaaaatc
     2881 gtcgtaagct actagtcaaa agtgaagaat taaaaaaata tcgaaatgat gtttctgctg
     2941 agatcgccca attgaagcgt gcaaaagaag atgcttctgc taaaatcgct gaaatgaaag
     3001 aagtcggtgg caacatcaaa gcattggata ctgaaattgc tgaagtggat gaaaaattga
     3061 aacaaattgc aactactttg ccaaaccttc cccatgattc tgtgccagtt ggaacagatg
     3121 aagaggacaa tgtagaaatc cgtcgttgga gcgaaccacg tacatttgct tttgaaccaa
     3181 aaccacactg ggaaattgca gaaaatcttg atattttaga ttttgaacgt ggcgcaaaag
     3241 tagccggtag ccgttttgtc tattacaaag gattaggtgc tcgattagaa cgtgctgtct
     3301 acaacttcat gcttgatcag catatttacg aacacggcta tactgaaatg atcactcctt
     3361 atatcgtcaa cagtaaagcg atgtttggta cgggacaatt tccaaaattc aaagaagatg
     3421 ttttccaatt ggagaatact gatctaacat taatcccaac agctgaagta ccattaacta
     3481 actattataa tgatgaaatt ttagatggtg cagatttacc tatttatttc actgcactaa
     3541 gccctgcttt tcgttctgaa gccggaagtg ctggtcggga tacacgagga ctgattcgtc
     3601 ttcaccaatt caataaagta gaaatggtca aattttcgga tgtagagcat tcttacgaag
     3661 aattggaaaa aatgacagct gatgcagaag atatcttgaa aaaattgcat ttgccttatc
     3721 gtgtgatggc cttatcaact ggagacatgg gcttttctgc agctaaaact tatgacttag
     3781 aagtatggat acctgcacaa gatacttatc gtgaaatcag ttcttgttct aactgtgaag
     3841 atttccaagc acgccgcgca atgatccgtt atcgcgataa tgatggaaaa atccactata
     3901 ctcatactct gaatggttca ggacttgcag ttggccgtac agtagctgcc attttggaaa
     3961 actaccaaaa tgaagatggt tctgtcacag ttccagaagc gctcgttcct tatatgggga
     4021 atctgaaagt tattaaataa agaggctgtg aaaaagccgc ttagtttcga gtattaagaa
     4081 aaattttgtg aaaatggctt tccacatttt tgcataaatt ggacttaata ccgagaaact
     4141 ggcttttgaa caccatttat cgagaggttg tgaaatcagc gaattaacct gagtattaga
     4201 gaaaaaaatg gaaaaatagt ccctcatatt ttaccgtttg ttgaactaat accgagggtc
     4261 agctgattga acaccgttta gtggagaggt cgtaagccta ccccccagct cctagttaca
     4321 aggacaaatt caaaaaacag ctcttcatat tttatgaatg ttgtacttgt gattcggagg
     4381 tggcaaggga acactgttta ttcaaaaaga ggctgggaca gaagcgttta actccaagaa
     4441 ataagaagaa attcacgaaa attgcttttc aaatttttgt gaatttcttc ttatttccga
     4501 aggagttgct tctacttccg ccgtttatac gtgtttagag cgtgagacaa aagtgttctt
     4561 tacttttgtc ccacgctctt taatgttttt aagattcaac tgaatagttt ggtgcttctt
     4621 ttgtgatttg tacatcatga gggtgtgatt cttttaatcc actgccgctc atttggataa
     4681 attgcgcttc atcacgtaat tgctgtagat tggctgcccc aacataaccc attcctgaac
     4741 gcaaaccgcc gatcatttgg aaaataatat ctgcaacact tcctttataa gcaacccgac
     4801 cttcgattcc ttctggtact aatttatttg cttcattgac actaccttgg aagtaacggt
     4861 cttttgaacc tttttccatc gcgcctaatg agcccatacc tcggtatgtt ttgaatcgac
     4921 gtccttggaa gatttcgaat tcacctggtg attcatctgt ccctgccaac atacttccta
     4981 gcatcactgc atgtccgccg gcagctaatg ctttgacgat gtcaccagaa tatttgatcc
     5041 ctccgtctgc aataatcgct ttgccatatt gacgagcaac tgatgctgca tcataaatag
     5101 ctgtgagctg aggtacacct acaccggcta ccacacgtgt cgtacaaatc gatcctggtc
     5161 cgataccaac ttttacgaca tctacacctg catcatataa agcttttgtc gcttcagccg
     5221 tcgcaacgtt tcctgcaatc aatgttgcat ctgcaaaagt tgaacggatt tcttgaattt
     5281 ttcggatcac acctgcacta tgtccatgag ctgtatcgat gatgattgca tctgcccctg
     5341 cttctaataa agcattcgca cgctcaaatg tatcgcttgt tacaccaacg gctgctgcta
     5401 caagcaatcg accatgttca tcttttgctg catttggaaa ttcgatcact ttctcgatgt
     5461 ctttgatcgt gatcaatccg cttaaacgac cttcgttatc tacgattggt aatttttcga
     5521 ttttatgttt ttgcaaaatc ttttctgcat cttttagaga ggtacctact ggggcagtca
     5581 ctaagttgtc ttttgtcatc acatcagcaa tcggcatgtg ataatctgtt acaaaacgca
     5641 tgtcgcggtt tgtaattatc cctaccaatt ttctgttttc aagtgtttca acgattggca
     5701 caccactgat acgatatttg ctcatcaatt cttctgcgtc tgctacaaga tgctgtggcg
     5761 ttaagaagaa cggatcaatg attacgccac tttcagaacg tttgactttt cttacttcat
     5821 ccgcttgctg gctgatcgtc atgtttttat ggatcacacc tagtccgcct tgtcttgcca
     5881 tagcaatggc catcttgctg tcggtaacag tatccatact tgcactgatg attggaatat
     5941 tcaacttaat atttttagct aattgtacgc tcatatccac ttcgtttggt aacacgtgac
     6001 tttctgctgg tatcaataag acatcatcaa aagtaaatcc ttttttcgtg aactttgttt
     6061 cccagttgga catttttagt accactcctc taatttagtt tatctattaa aataattgat
     6121 tttttcattt aagtcaatga tctacttgga atcaagttag ctttctcata gaataccaat
     6181 ataaagcgtt tacatctgtg tatattaaaa gagattgtaa aaaagctgtc ctgcatcaag
     6241 taataagaac agccaatcaa aaacagcttc tcatgttttt gaattggttg ggtttattac
     6301 cgcagatgca aacttttgaa caccgtttag tggagaggct gtgaaaaagc tgcttagttt
     6361 cgagtagtaa gaaaaatttt gtgaaaatgg ctttccacat ttttgcataa attggactta
     6421 ataccgagaa actggctttt gaacaccgtt tatcgtaaaa agggactgtg acaagacttt
     6481 tgtcacagtc ccttactatt atttaactat ggataaacaa aatgaggttc tttactaccc
     6541 ctagatatct agcaaaatag ctgatctttt tgctagttta tcttcttggg acggataaac
     6601 ttccaataat cccgtatttt ttcttcaagt agtaacggat tgctaaagca gttgccccga
     6661 taatgataac tactactgga tctaatactg gattgataac tggtggcaat agtgttgtcg
     6721 cagaaaacag tacgacccaa agaagtgtcg ttccacctag gataagcaat gtcttaccca
     6781 ttcctggttt cttgcttcga tctgctccgg gatattcata ttgatagatg tatttgtaca
     6841 tcatataaaa gacccagcca ccgattgcag aacctagaac gagtgtagaa aagccataag
     6901 ctggcgttgt tccacgtgaa aacaatccgg taatcgcaat catgatactt aatagaccaa
     6961 gcaacagcaa cgtattatct aaccacatca ataaaggggt ggatgaattt tttacttctg
     7021 ctggcttatt gatgattgct tccgttcttt cagagactgt cccaaacaat tgacgggctg
     7081 ttcttccgcc tttttgttct gcaactaaag taggcagaat gtcatgaagg gcttttactt
     7141 tttcttcttc agacaaattc gctgcttcta aagatttttt caaatcaaaa atgtactgtt
     7201 gatttctttt ggttaatttt gtttctaaac tattgttttc ttttacgatt gctcgtaatt
     7261 gttctggttc cattatctaa tagtcctcct tagccgttcc ttttctcaac ggtcgtcaca
     7321 aaagacattc taccatattc tacagctagt ttgatagccc ttttacacat taaagcggaa
     7381 taacattacg tcgccgtctt gtacgacata atcttttcct tctaatcgaa cacgtccagc
     7441 ttcttttgct gcatgcatgt ttccgtaatg atccaagtct tcaaaagaga cagtttctgc
     7501 acggataaat ccgcgttcga aatctgaatg gatgattcct gctgcttgtg gtgctttgat
     7561 tccttttttg aatgtccaag cacgtacttc ttgttcccca gcagtaaagt aagtagctaa
     7621 gcctaacaaa tcataagcag cacggatcaa ctgatccaaa cctgattctt cgattcctaa
     7681 agcttctaaa aattccgctt tgtcttcttc atctaattct gcgatttctt cttctgcacg
     7741 agcactgacc acgatcactt ctgcattttc ttctgcagca aagttgcgca cttcttgtac
     7801 ataatgattc gcatctgcat ctgctacgtc atcttctgag acattggcta cataaagaat
     7861 tggttttgtc gtcaataaaa atagtgattt aacgattttt tgttcttctt cagtaaattc
     7921 gatcgttcgg gcagattttc cttcttccag tacaggtttg attttatcca gtacagctag
     7981 ttctgccact gcatctttat ctttggtttt agcaactttt gctacacgag tatagcgttt
     8041 gttgatagat tctaagtctg ctaacactag ttccaagttg atcgtatcaa tatcttctag
     8101 tggatcaaca cgtccttcta cgtgagtaat attatcatca tcaaaacagc gaacaacgtg
     8161 acaaatagcg tctacttggc ggatatggct taagaattga tttcccaaac cttctccttt
     8221 acttgcgcct tttacgattc cagcaatatc tgtaaattca aaagtcgtcg gaacagtttt
     8281 tttcggatgt acaagttctg ttaagcgttg caaacgccag tcaggtactt ctaccatccc
     8341 tacgttgggg tcgatcgtcg caaagggata gtttgctgct tctgctcctg cttttgtgat
     8401 cgcattaaac aaagttgatt ttcctacgtt gggcaaccca acaattccag cagtcaatgc
     8461 cataaattta ttcactcttt ctctgcattt ttttcaatta ctttttttaa tttcttttca
     8521 aaatctcgac gggtcatcat gacgatatgt ccgcaattca tacatctgat tttgatatct
     8581 gcgcccattc gaatgacttc ccagcgattc gtctggcaag cgtgaggttt tttcatttca
     8641 acgatatcgt gcaagtcgta cataaaaata gcctcctttt tgtttattct tcatcaaatt
     8701 gaatattcaa gatatcaaga attcgagtca aatctttttg agaaagatat tcaatctcga
     8761 tcttaccttt cccatttttt tcttggatag ctacacttgt accaaattta tccatcaaac
     8821 gatcttcgct ttctcggata taatatggtt tttcctttgc tagccttggg atttttttct
     8881 tttcttttcc atcattttga tttaactcag aaacaagctg ttccagttgg cggacagtta
     8941 aattttcagt tactgcacga ttggctaatt tcaagatcaa tgttttattt tttaaaccta
     9001 acagcgtacg ggcttgtccc atagataaac gctgatcttg taccatttcc ttgacaagat
     9061 ccggtaaaga aagcaagcgc aaataattgg cgatataagg tctgctcttt cctaaacgac
     9121 tagctacttc tgcttgtgtt agtttcaggt ttttcatcag catctcatat gcttctgctt
     9181 cttctaacgg attcagatct tctcgctgca agttttccag cactgctact tgcatcatcg
     9241 cttcttcgtc aaagtctcta acgatagcag ggatcgtttc tttttcagct agtttggacg
     9301 ctcgaaaacg tcgctcccct gcaataatct catatccttt tactgcggaa cggcgtacga
     9361 tgatgggttg aaagacacct gattgttcaa tagaattagc tagttcctgc aaagatactt
     9421 cttcaaatgt tttacggggc tgatacggat ttggtcgcag ctcattcaat ggtagctgga
     9481 caacggtatc atcttttaca tccaccgtct ctaagttttc taaatcttga aacagtgcat
     9541 cgattcctcg gcctaatcct ttacttttct tcacgactta ccacttcctt tgccaatgct
     9601 tggtagactt cagctcctcg agaacgtgga tcgtagtcga tgatcggttt tccatgacta
     9661 ggtgcttctg ataaacgaat atttcttgga atgatcgttt cgtaaacttt ttcgcggaag
     9721 tatttacgta cttcttctac gacttcattt cccagatttg ttcgtgcatc atacattgtc
     9781 agtaagactc cctcgatctc taattctgga ttaaagtgtt tttggactaa acgaaccgta
     9841 ttcagtaatt ggcttaatcc ttccaatgcg taatactcac actgaacagg tatcaaaatc
     9901 gaatcacttg ctgtaaatga attgattgtc agatggccaa gagaaggcgg gcaatcgatc
     9961 aaaatataat catactgact gcttacttct gctaaagatc cttttaatct tgattctctt
    10021 gccatcattg acgtcaattc gatctctgct cctgctaatt ggagagtcgc aggcacaata
    10081 ctcaaatttt cgtgttcagt gatcaaagtt gcctcatcaa ttggcaattc attgactaga
    10141 acatcataga tgtctcttgt cacatcaggt tttctgatcc cgacaccact ggtagcattt
    10201 ccttgtgcat ccatatccac tagcaatact tttttgccaa ggctagcaag acaagcacct
    10261 agattcaccg tcgtcgttgt tttgccgacg ccaccttttt ggttcgcaac agatattatt
    10321 tgtgccatct cttctcctcc tatttgattg gctgcttatt aggcagtccg ggctttcttg
    10381 ggtatttctt tggggttggt ttcttttttt gaataacgat gatatgccgt tgatcagaga
    10441 cattcggcaa ggtaaccgcc tcttctttga ggaatttacc acctaataaa gcaatcgctt
    10501 gttttgcttc tgtcaattcc tcttcacttt ttgctgcttt caatgcataa aaatagcctt
    10561 cttgtttagc caagggcaaa cacaattccg ctaacacatt caaacgagca accgcacgag
    10621 cagtgacaag gtcaaatgat gcacgaaagt gaggattttg cccaaatgtt tctgcccggt
    10681 catgatataa ggaagcatcg attcctaata atcgggttaa ctcagataag aaggttattc
    10741 gtttattcag agaatcaacg atcgttatct gcagttctgg aaaggcaatt tttaaaggta
    10801 tgctaggaaa ccctgccccc gaaccaacat cgcataaaga taaaggttgc gtaaagtcta
    10861 cttcaaatgc taatgtaagt gaatcgtaaa aatgcttcag atatacgtct ttttcatccg
    10921 taatagctgt aaggttcatc ttttcgttcc attcgactaa tgtccgataa taaatagcga
    10981 attgattcat ttgatgatcg gtaagctcta tccctttttc tgccaaggat tggcgaaatt
    11041 cttctggtat cataaatatt cctttcattg tgaaacagca attgtttcac agattctctc
    11101 cctactttaa cgcaaaatct ctagacatgc aatccttcca tatggattct taaaaatttg
    11161 aaaagtaaga aaagccgata gactttttct ttctagtatg actcataaaa tatacacaga
    11221 agagagaact agctttgagc aatttctgca aaaactagtt ctctcttcta tttattatta
    11281 cgtattacgc tggttcactg actttcgcaa tcttcccttg ttcgatatag accattagga
    11341 tacttaggtc tgcagggttt acgccactga ttcggctagc ttgggcaatt gtttctggtt
    11401 ggattttctg caatttttgt tttgcttctg ttgctaatcc gttgattgct gcgtagtcga
    11461 tgttttctgg aatctttttc gcttccatgc gttttaattt ttctactttt tccatggctt
    11521 ttttgatgta gccttcatat ttgatttgga tttctacttg ttcgatttct tttcgtgtca
    11581 attcttcatt ttctggaata aaccgtagaa gttctgcata agtaatctca ggacgtctta
    11641 aaaagtcgct tgctagtaca ccatctttta atggtgccgc attaatcgtt gctaaaaagt
    11701 cttggacttc ttctgttggt ttgattcgta cattcatcaa acgtttgatt tcttgttcaa
    11761 ctgcttcttt tttcttcaaa taattcttgt attgatcttc ttttactaag cctacacggt
    11821 gacccatttc tgttaatcgc aaatctgcat tatcatgacg taaaagcaga cggtattcag
    11881 cacgtgaagt cagcaaacga taaggttcgt tcgttccttt tgttaccaaa tcatcgatca
    11941 tgacaccgat gtaaccgtca cttcgtttga tcactaaagg ttcttttccc tgaactttca
    12001 aagctgcatt gataccggca atcagccctt gtccagccgc ttcttcataa ccgcttgtac
    12061 catttgtttg tccagctgta taaagatttt caacgacttt agtttctaat gtcggacgga
    12121 gctggtgtgg ctctactacg tcatattcga tagcatatcc tgtacgcatc atttctactt
    12181 tttctaatcc agcaatagaa tgaagcatgt tgacttgaac atcctctggt aaagaagtag
    12241 ataaaccttg aacatagact tcttccgtat ttaagccttc tggttccaaa aataattgat
    12301 gacgtggttt gtctgcaaaa cgcacgatct tatcttcgat ggatggacaa tagcgagcgc
    12361 ctaccccttc aacgatccct gtaaacattg gggcacgatg taagttgtct cgaataattt
    12421 ggtgagtcgt ctcacctgta taggtcagcc agcaagattc ctgatctagt ttataggcac
    12481 tatctggtgt gctgaagcta aaatgattcg gctctttgtc accaggctgc tcttctgtaa
    12541 cagaatagtc gatcgtactt gattttactc gaggcggtgt tcctgttttg aaccgttcga
    12601 tagcaaagcc aagttctttt agatgattcg ctagtccaat agaaggttga gagttattcg
    12661 gacctgatga atatttcaat tcaccgataa tgatttctcc gcgaagtgct gttccggctg
    12721 taataacaac tgctttggcc cggtagctcg ctcctgtgga cgtcactact ccatgacaaa
    12781 caccatcttc gacgatcagt tcttcgacga ttccttgtct tagcgtcaaa tttggttctt
    12841 tttcgatcgt atgtttcatt tctgttgaat aagcatgttt gtctgcttgt gctcgcaatg
    12901 ctcggacagc tgggcctttt cctgtgttca acatacgcat ttggatatag gttttatcga
    12961 tattcttacc catttctccg cctaacgcat cgatttctcg aacgacaacg cctttagctg
    13021 gtccgcctac agaaggatta catggcatga atgctaccat gtccaaattt attgtcagta
    13081 ataatgtttt tgatcccata cgtgcagcag caagtgcagc ttctgaaccg gcgtgtcctg
    13141 cgccaacaac gatcacgtca tacgcatctg cttcataatg ttccaatcgt ttgttcctcc
    13201 ttttattttc ctaaacaaaa ttgactgaat aattgagtga ttagctcatc ttgtacactg
    13261 tcgccgacaa cttctcctag atagtcccag caacgcgtca tatctatttg aacgagatcc
    13321 acaggcattc ctgaatcaat acctgcaatg acttcttcaa gtgagaggga agctttttct
    13381 aacaacgcga tatggcgggt attagaaaca taagtagcgt ctctttctcc tgtttctccg
    13441 ctgaagaata aatcagaaat agctgattcc aaacgatcta gtccgtcgtt tttagctacg
    13501 gaaattgaaa aaactggttc attttcgatc aatttcttca atttttcttg ttccagctgt
    13561 gctggcaaat ctgttttatt tagaagaatg atccgtttca atccacttgt cgcttccaat
    13621 aattgttcat cttctgctgt cagtggttcg ctttggttta gaacaagtaa gatcaagtct
    13681 gcttctgcta atgcttttcg gcttctttcg accccgatcc gttccaccac atcttctgtt
    13741 tcccgaattc cagctgtatc gattagcttg agcggtaccc cccgaacatt gacatactct
    13801 tcgattacat ctcgagtcgt tcctgcaata tccgtaacga ttgctttttc ttctcttaac
    13861 aaatggttta acaaacttga tttgccgaca tttggccggc cgatgattgc cgtacttaac
    13921 ccttcccgca gaaccttccc ttgcttggat gtagcaagta gcgcttggat tctttgttgg
    13981 atcatctgcg ctttttcaag caataacttc gtagtcaatt cttcgacatc atcatattct
    14041 ggataatcaa tatttacttc tacttgtgct aatgtctcta aaatctcttg tctcaatgag
    14101 cggatcaaag cagacagatt gccgtctaac tgattaagag ctagtcccat tgctttgtct
    14161 gtttttgccc gaatcaaatc catcacagct tctgcctgtg aaaggtcaac acgaccattc
    14221 aagaatgctc gttttgtgaa ttcgcctggc tcagctaatc ttgcaccttc tcttaacaat
    14281 agctgcaaaa tttgattgac tacaacgatc ccaccgtgac aattgatttc tactacatct
    14341 tcccgcgtaa atgtctttgg tgctctcatc acggagacca tgacttcatc gacaagctga
    14401 ttgctcttgg gatcaacgat atgtccataa tggatcgtat gacttggtac ttctgataat
    14461 cgtttgtttc cagattgata aactttatct gctagttgca cagcttgatc gccacttaaa
    14521 cgaacgatac tgattgcacc ttctccaggt ggggtagaaa ttgccgcaat cgtatcaaat
    14581 tctaaagtaa tttcagccat tttttcactt cctttttcat tttgcgcaca aaaaaagtgc
    14641 ccactccacc ctttatgcac aataaacggt gaacttcgca ctttgactgc ttgttcttga
    14701 ttaaattctg gtcatagatt atcacacttg ctataaggaa tcaagtgttt aaaacaatta
    14761 gatgaagttg agcctgttct gcttccatta ccttatattt aattgtcctt ggatgtctct
    14821 ttgcgttctt tggtttattc gaagagaaga tactgtataa acaaggcttt atctttctta
    14881 aaaaaaacgg tacactaaat ggtagcgcta tctaaactat acacttttga aaggagaaaa
    14941 tatgtgagcc cgttcagatc tgctgcattg gaagaagaaa gtgaccctcg gatcaacgat
    15001 gtcgttttat ctacagggaa tatcaaccag aaatatatcg tccgcgatat cgtttttgtt
    15061 gtggaatgct tagaagctga tttgtttgac ccatcttttc ccaaagatga ccttttctta
    15121 acaacgaaga ggaaattaaa gacgaaggta cttgaatatg gtgcgaatgc agtaatcaac
    15181 tgtcattttg agtatcagca aaaaaaacgc gacggaaagt attttctgga actttttgct
    15241 tatggaacag tgatccaatt tatccaaaca acgattggtt aaatgaaaca gacaaaaaaa
    15301 gcggatcaaa actgaagaca gtttcgatcc gcttttttct atcaaagtta gtgactactt
    15361 catcgtatac tctgataaga atgatttcta gtaaaatata cttggtttga aaaactttgt
    15421 caacgtgatt tattcaaaaa gtaatggaat caataatatt ttttagcagg ttctactact
    15481 aaataacgat aaggttcctc accttccgaa tgtgttttta catgttcatc tttgcttaat
    15541 gcagcatgga tttgtttacg ttcaaatgca ggcattggtt ctaagaaaac aggctgccct
    15601 gtccgtttta ctttttcagc cgtgcgatca gctaaacgct gaatgatctc ttggcgcttt
    15661 tcacggtaat tgcctacatt gactactaca gacagcttat ttcttgctaa acgatggatg
    15721 aagacttgtg ccagatattg caatgcattc agtgtttttc catgtttccc aatcagtatg
    15781 ccttgctttt gtgtttctaa atggaaaaca accatattat ttgattctct ttccattttg
    15841 acaagagctg gagcatcaag ctgttttgaa atttcagtaa gataaactgc caattccgtt
    15901 aaagctgctt catcacctag ctcacttact tcttctgttt tttcttcaag aatcgtttcg
    15961 ttgactacct cgatctcagc tgttggttct tcttccaaaa tttcttctac agtctgttct
    16021 actgtttcag aaactttttc gctgatactt ggctggatcg aaagccgtgc attctttcga
    16081 cccattccta ggaaaccttt ttttccttct tccagtattt cgatttcgac ttggtctttg
    16141 gccaaaccca aatcagccag acctagttgg acagcttcgt ctattgttgc agcttcataa
    16201 atcggcatta gcattacctc ccctattatt tccgtttttt cttaggattc attgctttct
    16261 taagagcacg ttctctttcg cggattctcc gtgcttcttc ttcacgctct tttcgaattt
    16321 tgaaagggtt gttgatcagc agcgtttgta caacttggaa ggcattggaa actacccagt
    16381 ataatgaaag accgctggct aaattgacac ccatcactaa aatcattaca ggcatcacga
    16441 aattcatgat cttcattgat gcattcgatt caatttgact catgctagat aaatacgtac
    16501 ttgcaaaagt aaagattgct gctaaaatcg gcaaaataaa tgttggatcc gcgttcccta
    16561 agttcaacca taagaaatgc ccttgtttca actctggtac acgtgaaata gattgccata
    16621 acgccatcaa gattggcatt tgtactaata aaggaagaca accagcatat ggattgacgc
    16681 cattttctgc atataaacgc tgctgctctt ctcttaactt actttgtgtt tccggatctc
    16741 ttgaactata ttgttgctgc agtgctttta atttcggttg taattcttgc gttttacgca
    16801 tgctctttgt ttggaaatgc atcaacggca ataggatcac gcggatgatc agtgtaaata
    16861 aaatgatccc tatccctgca ttaccaaatg acaacgcctt gatcgcctca gcaaagtaat
    16921 aaacaatata acgatcccaa attcctgtac tctgtgcgct gacttcccca gttccgcaac
    16981 cggataagac gatgaccaaa ctaacgatac cggccatcaa aagtaaacgt ttgtatttct
    17041 tcacttactc ttttcctctc tgtcgattat ttttgcaagt tttaagacgt ggactaaatt
    17101 ggagcgaacc tcatcataag tcaacccctt cacacttggt ctagcaatca caataaaatc
    17161 caactccgga tcaatttctt cttttataga ataaactgct gctcggattt ttcgtttgac
    17221 ttcattgcgg tttaccgcgt tgccgacctt ttttcctaca gataaaccaa ctcgaaaatg
    17281 aggctgtccc ggtttttcca aacggtaaac gacgaatttg cggttagcaa aagaatctcc
    17341 cgtatggaac acggcttgaa actctcgttc tttttttact cgatacgctt ttctcattag
    17401 ttttctttcc ttttctatct ctcacttacg ctcataatca taccataatt acgattgtcc
    17461 tttcatcatt tccctaaaaa agagtataaa aaaaaccact gaaaccccag tggcctaagc
    17521 tgaaagaact tttcttcctt tgcgacgacg gctagctaac acacgacgtc catttttagt
    17581 actcatacgt ttacggaatc cgtgaacttt ttgacgttta cgtttatttg gttggtatgt
    17641 tcttttcata tattccacct ccaaaatgtt cttatctatt ttataaacac agacatactt
    17701 tgatagtata acgacaaagt agtgattttg tcaatataaa atagaagatc gattgttaaa
    17761 acttatccac aagattattt ttttctatgg gaatcctatg tggaaaaagt tatgcacagg
    17821 gattatccac agataataaa aggctggttt tacacagaaa acacacgtgt aaaaaaagta
    17881 atccacaatc aggcgatttc ctgtgtattc cgtctcctaa ctattggtat atctgtattt
    17941 tttatccaca gacgcaagag gaaatctatt ggtttttacg agttttccac ctgtgcatta
    18001 gtggtgtgga aaactttttt tgacaactgt gtttttctct tttttctttt tgtcgattgt
    18061 gtggataatt gtaattaaaa aatttttttc ttcggtttct gtggaaaact attgaaaaaa
    18121 atgctaaatt agtactagtt attcactttt taatgaagga ggaggcctta tatggtatcc
    18181 ctcgatgctt tatggaatga attaaaagca acataccaaa aggatttatc gcctgctagc
    18241 tataatacat ggatcgaaac agcgaaccct cgaacccttg atcaaaacca gctcgttgtt
    18301 gaggttccta gcaagattca taaagaatat tgggaaaaaa acctggcgac aaaaatcgtt
    18361 gaagtcggtt atatgttatc aggaaatgag attatccctc gctttattac tggcgaagaa
    18421 gcagagcaag aagaagttat agaagaaaaa aatccaaaag ttgtggcacc tagtccgcta
    18481 aaaaaagcca tgctgaaccc taaatatacc tttgatacgt ttgtcattgg taaaggaaac
    18541 cagatggctc atgcagcagc gctcgttgta gcagaagatc caggttctat ttataacccg
    18601 ctcttctttt atggaggcgt gggacttggt aagactcact tgatgcatgc aattggtcat
    18661 caaatgctgc aaagtcagcc taatgccaaa gtaaaatatg taagcagtga aacttttgct
    18721 aatgatttca tcaattcgat ccaaaacaaa acagcagaag agttccgtca agaatatcga
    18781 aatgttgatt tactgctagt ggatgacatt caattttttg cagaaaaaga agctacgcaa
    18841 gaagagtttt tccatacttt caatgccttg tacaatgaag ggaaacaaat cgtactaacc
    18901 agtgatcggt tgccaaacga gattccgaaa ctgcaagaac gccttgtttc tcgctttgca
    18961 tgggggctgt ctgttgatat tacaccgccg gatcttgaaa caagaacagc gattttacgt
    19021 aaaaaagcgg cggctgaacg tctagagatc ccagatgaca cactaagcta catcgcagga
    19081 caaattgatt ctaacattcg tgaattagaa ggtgcgcttg ttcgagtgca agcttttgct
    19141 gcgatgaata gtgaagatat ttcgactagt ctagctgcag acgcactgaa aacgttaaaa
    19201 tcaggaaaag gacatcctca attgtcaatc ctgcagatcc aagaagaagt ggcaaagtat
    19261 tatcacatcc aattgaaaga tctcaaagga aagaaacgcg tcaaatctat tgttgttcct
    19321 agacaaatcg ctatgtattt ggcacgtgag ctcacagata attctttacc gaaaatcgga
    19381 gctgaattcg gtggcaagga tcatacaacg gttatccatg ctcatgaaaa aatccagcag
    19441 ctgatggatt ctagtgtcag tatgcaaaat gaagtatcag aaatcaaaaa ctttttacta
    19501 aattaatttg tcgattctat caactgaaaa tagcatgtgg ataaaagaaa aaaacgttca
    19561 aaagttatac acactttttc cacagctgtt ttttcgtttc tctcttacgt tttttgagtt
    19621 ttccacagaa tcaacatgcc ctattacttt tattattctt tttaactaat aataaataaa
    19681 caaacattca aaggagatac atcatgaaag ttactttaaa ccgagctagc tttatgcagg
    19741 aattgcaaac tgttcaacga gctatttcaa gcaaaaccac gatccctatt ttgacaggtg
    19801 taaaaatcac actgacacaa gaaggtttga ctttgacggg gagcaacgct gatatatcaa
    19861 ttgaaacttt tttgtctgtt gaaaacgaaa aagcaaatat gcaaatcgaa tctactggtt
    19921 ccattgtttt acaagcacgt ttctttagcg aaatcattcg gagacttcct gaagaaacat
    19981 ttactttaga agttttagaa aataaacaag tagcgatcac ttctggaaaa gcgaatttta
    20041 tcgtaaatgg attagatgca gataactatc ctcatcttcc tgttgtcgaa agccataacc
    20101 agatgaaatt acctgtacac gtattgacta aactaatcaa cgaaacagtt tttgctgtct
    20161 cacaacatga gagtcgtcca atcttgacag gtgtgcattt cattttatct gataattctt
    20221 tattagctgt agctactgat tctcaccgtc taagtcaacg cgtgattcca gtagaacaag
    20281 cggctgatca ttttgatatt gttattcctg gaaaaagttt gatcgaatta tctcgctcat
    20341 taacaaatga agaagaaatc gtcgaaatca gcattatgga aaaccaagtg ttgttcaaaa
    20401 cagaaacgat gtatttctat tcccgtttgc tagaaggaaa ctatccagat accaatcgtt
    20461 tgattccttc aagttttaat acagaggttg aattttctgt tcctagcttt ttagcggcga
    20521 tcgaacgggc ttctttactt tctcatgaag gccgtaacaa catcgttcgt ttatctattc
    20581 gtccagatgc ggttgtttta tacggaaatt cgccagaaat cgggaaagta gaagaaagct
    20641 tgagttatac agcaagcagt ggcgatccat tagatatttc tttcaatccg gattatatga
    20701 aagcagcgtt gcgtgcattt ggagatatga gcatcaaagt gaaatttatc tctgctattc
    20761 gtccatttac attggaacca acggaagatg gcgttcaatt tattcagctg attactcctg
    20821 tacgtacaaa ttaacaatga ttttcaatgt tcttgaaaat ttaattgaaa agaaatagaa
    20881 aaaataaaat tttaaacaga aggccctaaa atctctttta aggctttctg tttatttttg
    20941 tttaaatcac cttttttaga ttaaatcgtc ttatatggaa aaatatatgg ataaatttgt
    21001 tttattgcga aaaaaaaggt ataatagagt atacttttaa taatgaaaat gagggatggg
    21061 cattgaaaca acaagtcatt ctaaatacgg aattcatgac gttgggccaa atgttgaaag
    21121 aagtcacagt tatcggtagt ggcggacaag caaaatggta tttggcagaa aacactgtgt
    21181 tagtcgatgg cgaaccagaa aatcgtcgtg gacgcaaact ttatcccggc atgatggttg
    21241 aagttccaga agtaggaact ttttttatgg taaaaaaagg aaacgacgat aatgaggctg
    21301 aataagctgt atttaaaaaa ttatcgaaat tacgaagagc tgaacatcga attttccaag
    21361 aacctcgtca tctttttagg agaaaatgcg caaggaaaaa cgaatctttt ggaaagtatc
    21421 tacgtattag ctatgacgcg aagtcatcgg acaagtagtg agcaggaatt gattggctgg
    21481 actgacgatc aggcgatgat tcaaggagag atcacaaaag gttcatccac tcttccatta
    21541 gaaatcttat tatctaaaaa aggccgaaaa accaagatca accatatcga acagaaaaag
    21601 ttaagtactt acgtgggaca aatgaatgtc attttatttg caccagaaga tctttcttta
    21661 gtaaaaggca gcccgcagac caggcggaag tttttagata tggaattagg ccagatcaat
    21721 ccaatctatc tttatgatct tgtccaatac caagcaattt tgaaacaaag aaaccagtac
    21781 ttgaagcaat tgaatgaaaa aaaacaaact gatttgcttt atttggatat tttgtccgaa
    21841 caactagcag cgtttggcag caaagtattg aaagcaagag cacagttcgt caagcgattg
    21901 gagttttggg ctaattcttt gcatcagcaa atcacccatc aaaaagagca gctggaaatc
    21961 gagtatctca ccgcagtcga ttctttagaa acacatacgc aagagcaaat ccaagaacaa
    22021 tttctcgcac ttttgaacca aaataaaaag aaagaccttt ttcggggaac aacgacagtt
    22081 ggtcctcatc gtgacgatct ttcctttttt attaatcaga aaaatgtgca gacttatgga
    22141 tcgcaaggac agcaaagaac aactgcacta agtgtcaaat tagcagaaat cgatttgatt
    22201 aaagaagaga caggggaata tccaatcctt ttgttagatg atgtgatgag cgagctggat
    22261 gataatcgtc aactccatct gctagaaaca atcgaaggaa aagttcaaac gttcttaaca
    22321 acaacgacac ttgaccatgt aaaaaataaa atgactgttg aacctgagat tttttatgtt
    22381 caacaaggga aactaaaagg agaagaaact gaatgacaga agaaagaagt ttagtagaac
    22441 gcgctaaaga gtatgatgcc agtcagattc aagtacttga aggacttgaa gccgttcgta
    22501 aacgacctgg catgtatatc ggttcgacta gttctgaggg tctgcatcac cttgtttggg
    22561 aaattgtcga taattcgatc gatgaagtat tagcaggttt tgcaacgaaa atccatgtga
    22621 ttattgaaaa agacaacagt atcaccgtta ttgacgacgg tcgggggata cctgtagata
    22681 tccaggcaaa aactggacgt cctgcagttg aaacggtctt tactgtttta catgctggag
    22741 gaaaattcgg cggtggtggg tacaaagttt ctggtggact tcacggagta ggatcttctg
    22801 ttgttaacgc gctttctaca cagcttgatg tcaaagtata caaagaagga aatgtgtact
    22861 atcaagaata tcgtcgtgga gctgtagtgg acgacttaaa gattatcgaa caaacggatc
    22921 gccatggaac aacggttcac tttacgccag accctgaaat ttttacagag acaactgagt
    22981 ttgatttttc aaaattggct acccggatcc gtgaacttgc tttcttgaac cgcgggatgc
    23041 gtatatcgat tgaagacaaa cgagaagaag aaccagtcat caatgaatac cattatgacg
    23101 gcgggattaa aagttatgtc gaatacttga atgcaaacaa aaccgtgatt tttccagaac
    23161 ctgtatattt ggaaggtgaa cagcaagata tcgcagttga agtctctatg caatatacag
    23221 acggctacca ttccaatatc atgagttttg ccaacaatat ccatacgtat gaaggcggaa
    23281 cacatgaatc aggattcaag actgctttaa ctcgtgtgat aaatgattat gcccgaaaac
    23341 aaaagctgat gaaagaaaat gacgacaact tgaccggtga agacgtgcga gaaggattga
    23401 cagctgttat ttctatcaag catcctgacc ctcaattcga aggacaaaca aaaacaaaat
    23461 tgggcaattc cgaagtacgg acagtcactg atcggttatt tagcgaacat ttcatgaaat
    23521 tcttgctgga aaacccaagt gtgggccgtc aaattgttga aaaaggatta ctagcttcca
    23581 gagctcgtct agcagcgaaa cgtgcgcgtg aagtaacgag acgtaaaggt gctttagaga
    23641 ttagtaatct gccaggaaaa ttagcggact gctctagcaa cgatcctgaa aaatgcgaat
    23701 tgttcatcgt cgaaggagat tcggccggcg gatcagctaa gcaaggtcgt aatcgagagt
    23761 ttcaagccat cttgcctatt cgtgggaaaa tcttgaatgt tgaaaaagca agcatggaca
    23821 agatcttggc aaatgaagag atccggtctt tatttactgc tatgggtaca gggtttggag
    23881 aagattttga tgtatcgaaa gctcgctacc acaaattagt tatcatgact gatgccgatg
    23941 tcgacggcgc gcatatccgt acattgctgc tgactctttt ttatcgttac atgcgtccta
    24001 ttgttgaagc tggatacgtc tatattgctc agccaccttt atacggggta aaacaaggga
    24061 agaatattac gtatgtacaa ccaggaaaaa atgcagaaga agagttgaaa caagtcgttg
    24121 cttcacttcc tgcctcacca aaaccaagcg ttcagcgtta taaaggactt ggagaaatgg
    24181 atgatcatca actatgggaa acaactatgg acccaagcaa tcggatgatg gcccgcgtca
    24241 gtgtggacga tgctattgca gcagatcaga tatttgaaat gttgatgggg gatcgagtag
    24301 aaccccgtcg tgcattcatc gaggaaaatg cacactatgt gaagaactta gatatttaat
    24361 catgaaggag gaaaaagaat gagtgaagaa atcagagaaa acatccatga tgtcaatcta
    24421 accagcgaaa tgaaagactc cttcatcgat tatgcgatga gtgttattgt cgcacgtgca
    24481 cttcctgatg tcagagatgg tctgaaaccc gttcatcgcc gtattttata cggcatgaac
    24541 gaattaggtg taacaccgga taaagcccat aaaaaatctg ccagaatcgt cggagatgtc
    24601 atgggtaaat accatcctca tggagactat gcgatctatg agtcaatggt acggatggca
    24661 cagccattca gctaccgtta tatgctagtg gatggtcatg gaaactttgg ttctgttgac
    24721 ggtgacggtg ccgccgctat gcgttatacc gaagcacgga tgagcaaaat cgctacagaa
    24781 atgctgcgtg atatcaataa gaacactgtt gatttccaaa gcaactatga tgatacagaa
    24841 aaagaaccag ttgtactacc tgctcgcttc ccgaatcttt tagtgaacgg aacaacgggg
    24901 atcgccgtag gtatggccac gaatatccca cctcataact tgtctgaggt agtagcggct
    24961 atcgatttgc tgatggataa tcctgatgtg acgaccaatg aattgatgga agtattacct
    25021 ggaccagatt ttccaacagg tggtttggtt atgggaaaat caggaatccg aagagcgtat
    25081 gaaacaggta agggttctat taccgtacgt gcaaaagttg agattactga gatgccaaat
    25141 ggtaaagaac ggatcatcgt gacagaattg ccttatatgg tcaataaagc aaaattgatc
    25201 gaacggattt ctgaactgca cagagacaag cgtatcgaag ggatcacaga tttgcgggac
    25261 gaatcttccc gtgaaggtat gcggatcgtc attgacatcc gtcgtgacgc cagcgcatct
    25321 gttatcttga ataacttata taaattgaca tccttgcaaa attcatttgg attcaatatg
    25381 ttggcgatcg aaaaaggtgt gccgaaagtt ttgagcttga aacaaattct tgaaaactat
    25441 atcgaacatc agcgcgaagt catcacaaga cggacagtct ttgaaaaaga aaaagcagaa
    25501 gcaagggcac acattttaga aggtttgcgt attgctttgg atcatatcga tgaaatcatt
    25561 gcgattatcc gcgggtctaa atcagatgac gaagcaaaag cgacgatgat cgaacggttc
    25621 gatttatccg atcgccagtc tcaagctatt ttagatatgc gtttacgccg attgaccggt
    25681 ttggaacggg aaaaaatcga aaatgaatac caagaactct taaaactgat tgaagattta
    25741 actgacattc tggcaagacc agaacgtgtt acagaaatca tcaagaatga attagcagac
    25801 ttgaatcaac gatttggtga taaacgacgc acagaattac tagtcggtga ggtcttaagt
    25861 ctggaagatg aagatttaat cgaagaagaa gaaatcgtta ttacattgac aaacaatggt
    25921 tacatcaaac gtttggcaaa taatgaattc agagctcaac gaaggggtgg acgtggtgtc
    25981 caaggaatgg gtatccacaa tgacgacttc gtcaaaaatc tggtttcttg ttctacccat
    26041 gatacattgt tattctttac gaataacgga aaagtgtatc gggcaaaagg atacgaaatt
    26101 ccagaatacg ggcgcacagc caaaggaata ccaattatca atttactagg tatcgattcg
    26161 agtgaaagca tccaggcaat cattgcggtg acgggagaag cagaagaagg ccactatctc
    26221 ttctttacga cgcgtcaagg tacagtaaaa cgtacttctg tcactgcttt tgctaacatc
    26281 cgaagtaatg gtctgatcgc aattggtttg aaagaagacg atgagctcgt caatgtgcta
    26341 ctgacagatg gtcaatctaa tatcattatt ggaacacata acggctactc tgtcactttc
    26401 aaagaagaag cagtccgcga tatgggacgt acagcttccg gggttagagg aatacgcctg
    26461 cgtgaggaag attatgtcgt tggggcagcc ttaatgaagg aagatcaaga agtattagtg
    26521 atcacagaaa aaggttacgg aaaacggaca aaagtatcgg aatatccagt gaaaggccgc
    26581 ggaggtaaag ggatcaaaac ggcgaacatc actgaaaaaa atggtccact agctggattg
    26641 accactgttt ctggagatga agacatcatg ctgattacag ataaaggtgt cattatccgt
    26701 ttcaacgtat cgactgtttc tcaaactgga cgttcaacat taggcgtacg tttgatgaag
    26761 atggaagctg atacgaaagt tgtcacgatg gcagcagtcg aaccagaagc tgcagaacaa
    26821 acagttgaag aacaaggatc agaagaatag ccatcaagac taattgtgta agctagcaag
    26881 aatgaccgat taaaaaatct ggttattcct gctggctttt ttgattagtt gaatttctct
    26941 taaaatattc gaaactgttc attctgtatt gcttttgacc aaaatagagt gtataatcag
    27001 agcttgtgag tagtctaatt aattagataa ctctccttgc tcttaggaac ctaagagccg
    27061 aaagtccaca gggaggtgaa tatcaatgga aaacacgaaa tatgaaatca tgtacattat
    27121 tcgtccaaac attgatgaag aagcaaaaac tgctttaatc gaacgtttcg atgcaatctt
    27181 gaaagataac ggagcagaag ttatcgaatc aaaagattgg gaaaaacgcc gtttcgctta
    27241 tgaaatgaac ggttaccgtg aaggtatcta tcacatcgtt aaagtatctt ctccatcatc
    27301 agcaaatgct gtcaatgaat ttgaccgtct tgctaaaatc aacgacgata tcattcgtca
    27361 catgattgtt aaagaagaag cttaattgtt gtttcatgtg aaacaataga aaacgatgag
    27421 aggagatgac tattttgatt aataatgttg tattagttgg acgtctgacg aaagatccag
    27481 atttgcgtta cacagcaagt ggtacggcag tcgcaacatt tactttggct gtgaatcgta
    27541 attttacaaa ccaaagcggc aatcgcgagg ctgattttat caactgtgtg atttggcgca
    27601 aatcagcaga aactttagca aactatgctc gtaaaggaac acttttaggt gttactggac
    27661 gtatccaaac tcgttcttat gataatcagc aaggacaacg tgtatacgtg acagaagtag
    27721 tagctgacaa cttccaattg ctggaaagtc gttctgcatc tgaaaatcgc caacaaagcg
    27781 gcggattcca aagttctggt caatcagctg gcggatttgg cggaaacaat aactctaatc
    27841 aaacatctca atcatccaac ggtatgcctg atttcgaccg tgatacttct gacccattcg
    27901 gctcaagttc aacgatcgac atttcagacg atgatttacc attctaaacg aatttgatag
    27961 gagggaaata gaatggcaca acaaagaaga ggcggacgta aacgtcgtaa agtagactac
    28021 attgctgcta accatattga atatatcgat tataaagata tcgaattact aaaaagattt
    28081 atttcagaac gcggtaaaat cttgccacgt cgtgtaactg gtacaggcgc taaaaaccaa
    28141 cgtaaaatta ctgttgcaat caaacgcgct cgtatcatgg gcttgctacc tttcgttagc
    28201 gaagactaat aaatcctagg aattgaatga ctgatccttt tgatcagcta ttcaattcct
    28261 ttttttctgt aaaaataacc tctattcgtt tagtcgctcg aatgacgaaa gttatggtaa
    28321 aatcaaatta gtcatatatg ggaggaaaat tatggaaaat aaaaacaatc atttatcccc
    28381 gaaacctctc ttccttatcc tgtttatatt aatcgaagta ttggtagtct taattttacc
    28441 atttcgtttt gttagcgctt cggtcattct attgatgaat ttatggggtg ttcgctattg
    28501 gttgaaaatg aaacgttggc ttattttaaa taaacgtgaa aaaatccagc aggcttcgac
    28561 tgtagctgaa accaatctag cttatgtctc agaagtcatg cctgtaggag tgattgctta
    28621 tcagccatca agccaaaaaa tcgaatggct caatccatta gcattgaaaa tcaaagatca
    28681 aagtggatta acagaggaag aattgctaga tacttttttt actgcaaaag aaaaaaagaa
    28741 aaaccaagtc tatctagcag ataaaacatt tcagtttgat atggatacag taaagggtgt
    28801 tgtttatttt aatgatatta cccaaaacca gttgttaaaa ctccatcaga cgaacagtca
    28861 gccggtgatt ggtattttat ctattgataa ctatgcagat gccattgata aattggatga
    28921 caaagagatt tcttatttga actctttcgt cactacacta gtttctgact ggatgaatga
    28981 ataccatgtc tttttcaaaa gaatcaacgc agaacggttt ttcttcgtag cacagatgat
    29041 cgatctgaaa aagatgatgg ataaaaaatt tgatttgctg gatcgctttc gagcagaagc
    29101 tgaaaaacag agctcgccta tcacccttag tatggggatt gcttatggct caagaaccct
    29161 cgatgaaatc ggtggagaag ctcagaataa tttagatgtt gctttagtcc gtggaggcga
    29221 ccaagttgtc gtgaaagagg taaaagaagg agcaaaacct gtttattatg gggggaaatc
    29281 tgctaagaat gtcaaacgca cgcgtgttcg ttctcgagcg atgagtaccg ctttgcgtcg
    29341 gatttttgat gaaacaggcg atgtgtttat catggggcac aagtttccgg atatggatgc
    29401 tttaggctct gcttttggtg tcagttgtct ggcacgcttc aaccagaaaa aagcttacat
    29461 cgttatcaac gagaacgaga tcattccaga tgtagaacgg tgtcttgaag aaatccataa
    29521 gcatcctgaa ttagaaatgc aactaatctc tccagaacgt gccatagagc tgaaaaaaga
    29581 agaggacctc ctcgtgatgg tggattacca taaaccttct ctatcgatct ctcagggcct
    29641 atatgagcaa tttgataaga ttgtgatcat tgatcaccat cgtcgtgggg aagagtttcc
    29701 aagtaaacct ctgctgactt atatcgaatc ttcagcttct tcagcttcag agctagtttc
    29761 tgagctgatc caataccaaa gcagcaaaac aaatcgctta gatcgttttg aggccacatt
    29821 attgcttgca gggatggttg tagatacaaa tagtttcaat gtccgtacaa ctgcacgaac
    29881 ttttgacgtt gccagctatt taaggtcttg tggagcagac gcatccttag tacaatattt
    29941 attgagctcg gatttagcga catacttgga gatcagcaat ttgattgctg gaagcgacta
    30001 tgtgacttct gatattgttg tggcggcagg gacagacgaa aaagagtata atagtgttat
    30061 agcagcaaaa acagcagata cattgctttc gatgatcaat atcaatgcag ctttcgtcat
    30121 tacaaagcgg acagacggct tgattgggat cagtgccaga agtaccggca cgatcaacgt
    30181 ccaaatcatc atggaatcct taggtggcgg aggacacttc acaaacgcag ctactcaact
    30241 ggaaaatacg acagttgagg aaacaaaaca gaagctattt gctgcaatca aagaaaatat
    30301 gaatcaaata tatgataagg agtaatccaa atgaaagtaa tttttttaca agatgtaaaa
    30361 ggaaaaggta aaaaagggga catcaaagaa gttccagcag gttatgcaca aaattatcta
    30421 atcaaaaata acttggcaaa agaagcaacg aaaagcagta tcagtcagtt agcaggacaa
    30481 aagaaagcac aagaaaaaca cgaagctgaa atcttggaag aagctaaaaa gttaaaagaa
    30541 ttcttggaaa aagaagatac tgtagtcgaa ttgaaggcga aagcagggga agacagccgt
    30601 ttgtttggtt cgatcccttc aaaacaaatt gctgaagggt taaacaaaca atacaaaatc
    30661 aaattagata aacgaaaaat cgaattagaa aatccgatcc gttcactagg ctacacaaac
    30721 gtacctgtta agcttcatca tgaggtgacg gctacgatca aagttcatgt cgtacaagaa
    30781 taacaaatct tgatagcgat gttcagctgg taagtataac cagaaggaaa aaagacaaac
    30841 cgaaatttat ctgagaaaac agctgcagca atgaaagcaa aagctgcaaa tcgatttcag
    30901 attgatttcg gtttcttttt tgggaacagc aaaatagcat aaagaaaacg agtataataa
    30961 aagagaacta tttttttatt acactttcgg tacgaatgat gacttttctc tcattttctt
    31021 gtaaaatgaa gcaattgaag acaaggagcg atacgtatga atgaagtgtg gcaggatcga
    31081 atcccaccgc aaaatattga agctgaacaa gcggttttag gctctgtttt tcttgacgca
    31141 gaaacgatca ttgatgccat ggagtacata gagccaaaag acttttatcg tcgtggccat
    31201 caaattattt ttgagacaat gatcgagtta aatgatcgta atgaagcgat tgacgtggtt
    31261 actgtcaaag accggctgga acaagccaat ttgttagaag atgccggtgg tttaagttat
    31321 ctctcagact tggcattagc agttccgaca gcagcaaata tcgtctacta tgcgaaaatc
    31381 gttgaagaaa aatcattgct gcgtacattg atccagacag caacaggaat cgttacgaaa
    31441 ggattcgagc aaggagaaga agttcaaaca attttagatg atgccgaacg cagtatcttg
    31501 gaggtttcgg aaaaaagaaa tcgaagcggc tttttatcta ttgctgaagt actgaactcc
    31561 acgattgaaa atatcgatcg actagctcaa aacaatgaag aaatcacagg tctaccaact
    31621 ggctatcaag gtctggataa aatgacagca ggtttccagc ccgaagaact aatcattctt
    31681 gctgcacgtc cagcggtagg taaaacagcg tttgcattga atattgctca aaatgtagga
    31741 acaaaaacag atcgttctgt tgcgatattt agtttggaaa tgggggcaga atctcttgta
    31801 aatcggatgc tttgtgcaga aggttcgatt gaggccagcc atttacgtac aggtcagctt
    31861 tcggaagaag aatggtcgaa tctgatcgtt gcgatgggga gtctttctcg agcaaatatt
    31921 tttatcgatg atacaccagg gatcaaaatc tctgaaatcc gtgcgaaatg tcggaaacta
    31981 gcgcaagaaa aaggcaatct aggcttgata ttgatcgatt atttgcagtt gattgaaggt
    32041 aacggtaaag agaaccgtca acaagaagta tcagatatct ctcgccaact gaaaaaactg
    32101 gcgaaagagc tgaaagtacc tgtcattgct ctttcacagt tatctcgtgg agtagaacag
    32161 cgacaagaca aacgaccagt gctaagtgat attcgtgaat ccggatcaat tgaacaagat
    32221 gcggacatcg tagccttctt gtatcgagat gattattatg aaagagaagg cggagatgac
    32281 gatgatggaa acgagccaaa aaatgacaac gtcatcgaag tcatcatcga gaagaaccga
    32341 agcggtgcac gaggaacagt tgaactactg tttatcaaag aatacaataa gttttcgtcg
    32401 ctttcgccaa gagaagaatt ttaaagaaga ggttgtgagc cttgagcaat cccgagagct
    32461 ctcgtaagaa gatgcaagga gtaaccgcag gaacgactag agtacttaat ttaaacagat
    32521 ctccaaaggg attgtgacaa aagtcttgtc acagtccctt tttccgaata aacggtgttc
    32581 aatcagctga cccttggtat tagctcagca aacgtcaaaa tatgaggaac tattttgacg
    32641 tttgtttctc taatactcag gtcaaaacgc tgatcttacg acctcttttc cttaaacggt
    32701 gttctcctgc caactcctcg gtcacaagtc acaatatcat caaacatgag aagcagtttt
    32761 ttgaaattgc tccttgtgac tcagagttaa acggcaggct cacaacctct ttcttaaacg
    32821 gtgttcaaaa gcttgcatct gcggtaatac gcccaatcaa acgaaaaata tgagaagcta
    32881 tttttgtttg gctgttctta ttacttgatg caggacagct ttttcacaac ctctgattca
    32941 cggtgttcca aaagtagctt cttcgaaaaa taagtcgaaa tttcacaaaa atttgagaag
    33001 caattttcgt acattccttc ttatttcttg aagctgacca cttctgttac aacctctttt
    33061 ttaataacca ttctaaaaag ataaagttcg ttttttaagc gtttttataa aataaaaata
    33121 aaaatcattc gattttattt gaaatatgta tctattattg atacaatggt tcaggattaa
    33181 ataatataga acgaggtgtt cacatgtcat ctgtagtagt agtaggtacg caatggggcg
    33241 atgaagggaa aggaaaaatc acagactttc tcagtgaaaa tgctgaagtg attgcacggt
    33301 accaaggtgg cgacaatgca gggcacacaa ttaagtttaa cggtgtgact tataaactgc
    33361 atttgattcc ttctgggatt ttttataaag acaagatcag tgtgatcgga aacggcgttg
    33421 tagtcaatcc taaatcattg gttaaagaat tagcttattt acatgaaaaa ggtgtagaaa
    33481 caagtaattt gcgtatttct gaccgcgcgc acgtaatctt gccttatcat atcaaattgg
    33541 accaattaca agaagatgct aagggcgaaa ataagattgg tacaacgatt aaaggaatcg
    33601 gacctgccta tatggataaa gcagctcgtg taggtatccg tattgcagac ttattagata
    33661 aagaaatttt tgaagaacgt cttcgtttga atctagatga caaaaaccgt caatttgtaa
    33721 aaatgtttga ttcagaacca cttgattttg acgatatttt tgaagaatat tatgaatacg
    33781 gacaacaaat caaacaatac gtcacagata cttccgttat cttgaatgat gctttggatg
    33841 caggcaaacg cgtgttgttt gaaggcgcac aaggtgtaat gctggatatc gaccagggaa
    33901 cttatccttt tgttacttct tctaacccag tagccggtgg ggtaacaatc ggaagcggtg
    33961 tagggccttc aaaaatcaat aaagttgtcg gcgtctgcaa agcctatacg tcacgtgtcg
    34021 gtgatggacc atttccaact gaattatttg atgaaacagg agaacaaatc cgcgaagtag
    34081 gacgagaata cggtacgaca actggtcgtc cgcgtcgtgt aggctggttt gattctgtag
    34141 ttatgcgcca ttctaaacga gtttcaggta ttaccaacct atctttaaac tcgatcgatg
    34201 tattaagtgg attggaaaca gtaaaaattt gtacggctta tgaattagat ggcgaactaa
    34261 tctaccacta tccagcaagc ttgaaagagt tgaaccgctg caaaccaatc tatgaagaat
    34321 tgccaggctg gtcagaagac attacaggat gcaaaacact tgcagaactg ccagaaaacg
    34381 cacgtaacta tgtacgtcgt atctcagagc ttgttggtgt acgtatctca acattttctg
    34441 tcggacctga tagaacacaa acaaacatct tagaaagcgt ctgggcgcaa atctaaggat
    34501 aaaaacgacg actgaatcag gacgttctta ccagagcgag tcgaagtgtt tatctatacg
    34561 ataataataa cttgaacact gtttattcag aataagggac tgtgacaaaa accgtgtcac
    34621 agtccctttt tccgaacaaa cggtgttcaa aagcttgcat ctacggcact aagcccaatc
    34681 agatgaaaaa tatggagtgc tatttttatc tgattgttct tactgcttga tgcagagcag
    34741 ctttttcaca acctctccac taaacggtgt tctcctgcca actcctcggt cacaagtcac
    34801 aatattcaac aaacatgaga agctgttttt tgaaattgtt ccttgtgact cggagctaaa
    34861 cggcaggctc acaacctctt ggtaaacggt gttcaatcag ctgaccctcg gtattagctc
    34921 aaaaaacggc aaaatatgag gaactatttt ttccgttttt ttctctaata ctcaggtcaa
    34981 aacgctgatt tcacaacctc tgattcacgg tgttccagaa gtagcttcta cggaaataag
    35041 ccgaaatttc acaaaaattt gagaagcaat tttcgtaaat tccttcttat ttcttgaagc
    35101 tgaccacttc tgtcacaacc tcttattttg attagaactt gcgaaaataa accggttaaa
    35161 gtgtgtctag cgcaagagat ttgctataat agacgataag ggtaaaatgt aacgagaagg
    35221 ggttgaaaaa tgtgtatcaa attgtaacag attcttgttg tgatctgcca taccatgtat
    35281 tagcaaaaga aaaagttgat tttttatcaa tgaatctcca attgaatgat caagagttag
    35341 tagatgacct cggaaagaca ttcaattatg accgctacat gaatcagctg aaaaatggtg
    35401 cgatgcctac gacatcacaa atcaatgttg gccgatattt agaatttttc cgttcttatg
    35461 cggaaaaaca gattcctgtt ttatatgtgg ctttttccag tggattaagc ggctcttaca
    35521 gtagtgcgct tcaagcggtt gaattgctga aggaagaata tgaagaccct gagatctatg
    35581 tttttgatac aaaagctgca agtattgggg aagggctttt agtattggaa gctgtaagat
    35641 tgaaaaaaga aggtaaaagc atcgaagaaa ccctagagtg gctggaaagt aattatttga
    35701 aagtccattc atgggtaaca gtagacgatc tgaaatatct agaacgaggc ggtaggattt
    35761 ccaaagcaag tgctactctt ggcagtctat tgagtgtaaa gcctattatt acggtggata
    35821 aagaaggaag gctgacgaat gttgacaaag ttcgaggaag aaacaagtcg atccagacaa
    35881 tcgtcaatga gacggtaaaa acaatcgttg caccacttga tcaaatcgtt tatgtggctt
    35941 acgctgggga tctagagagc gcggagaagg caaaacggct tttggaagag cagataaaga
    36001 tgaaagatgt gaagctttat cccttaggac caacgatcgc cagtcacaca ggctatggct
    36061 gtatcgcgat tttttcgatg ggaatcagtc gataaaagca gtaactcatt ctgtaaaaaa
    36121 gcccagaaat tgattgtaac aaaaacagcg actcttaaaa gatagatgag catcctatct
    36181 cttagaggtc gctgtttttt tattttttaa gctaaatagg ccattatgcc cataactcgt
    36241 tcaattgttt ttgaatatct gcattttcca aaaattcatc atatgtcgta tcagctcgat
    36301 cgatcacacc tttatccgaa acggcaatga tgcggtttgc caatgtttgg ataaattgat
    36361 ggtcatgtga cgcaaataag attgagccag taaatgccat caagccgtca ttcaatgctg
    36421 tgattgattc taagtctaaa tggttcgttg gatcatctaa tacaagtacg ttagctttcg
    36481 aaagcatcat ttttgaaagc atgcaacgga ctttctctcc accagataaa acattcactg
    36541 gtttcaatac ttcttcacca gaaaacaaca ttcttcctaa gaagcttctt aagaaggtgt
    36601 tatcatcttc ttctttactt gcatattgac gcaaccagtc taaaatcgtt agatcagtat
    36661 cgaattcttt cgttgtatcc tttggtaaat aagaattact tgttgtcaca ccccaacgta
    36721 ctgttccagt atctggcgta atttcaccca tgataacttt gaatagcgtt gttgttgtta
    36781 tatcattttc tgcgacgaaa gcgaccttgt cttctttgct caaattaaaa gatatatcat
    36841 ctaaaatttt ctttccatca atcgttacac ttacattttc tacctgcaat aagtcgtttc
    36901 caatctcacg ttcaggtttg aatccaacga atggataacg tctagaagaa ggttggatat
    36961 cgtctaaagt aattttttca agcatttttt tacgtgaagt tgcttgtttt gattttgatg
    37021 cattcgcact aaagcgggca ataaattctt ggagttcttt gatttgttct tcttttttgg
    37081 catttgcgtt tgcttgaagt ttagccgcta gttgactaga ttccaaccaa aaatcataat
    37141 ttcctacata aagtttgatt ttaccaaaat ctagatccgc catatgtgta catactttgt
    37201 tcaaaaagtg acggtcatgg gatacaacga tcacagtatt ttcaaaattg atcaaaaatt
    37261 cttccagcca attgatcgat tggatatcca gaccgtttgt tggttcgtct agcaaaagaa
    37321 catcaggttt accaaatagt gtttgtgcta ataacacttt taccttttgt ccaccagtta
    37381 attcactcat ttgctgatgg tgcaattctt cagggatgtt cagaccttgc agtaaaacag
    37441 ctgcttctgg ttcagcttcc catccattaa gctcagcaaa ttctccttct aattcagctg
    37501 ctcgaatacc atcttcgtca gtgaaatcct ctttcatgta aatcgcatct ttttctttca
    37561 gcacttcata cagacgcttg tgtcccatga tgactgtttc aagtacagta tagtcttcat
    37621 agtcgtaatg gttctgtttc aatacggcca tccgttcatc tggtcccata gtaacggaac
    37681 ctgtggaagg ctgcagatcc cctgataaca ctttaaggaa cgttgattta cctgcaccat
    37741 ttgcaccaat caagccatag cagtttccgg gggtaaattt aatattcaca tcatcaaata
    37801 gtttgcggtc tgaaaagtgc aaactcacat catttacagt aatcaatttt tttcctcact
    37861 tttatcatta ttagttttga caagtcttat agaattatat aggggaaagg taaaagattc
    37921 aaggcaatcg aacattcttt gtgtttcacg tgaaacagaa tgaaaaaaat aaaaataaaa
    37981 gcggagcgaa aatagctact ataggattta ttagtttgca acagcataat ttttgacgtt
    38041 atttataaga gggagtacta taaaactgta aaagaaatga aagaaggatt gaaaatggat
    38101 gaaaaaagaa ccatcgactg gggctcattg attttgggaa ttttatttgt acttgtctca
    38161 ttgttatcat ttcaagaccc tgtcggaaat ttagttgcaa ttgtagtagt ctttgcgatt
    38221 tttgccttta ttaaagggat atttgaactg tttgtacgga acagattaaa agagctaaca
    38281 ggatataaag gaaagatgcc cttaataatc gggatcatcg atattctagt tggcgtcttt
    38341 ttcttattca atattggtgc aggagtcgca gcattgcctt ttgtttttgc agtatggttt
    38401 attgctgatt cagttttagc gttatttact gctgacttgg caagaggagt aagtgaagga
    38461 tattattggt ttacgatcat cgtcaatatt ttaggaattt tattaggaat tttcttattg
    38521 tttaatccaa tttcttctgc gttgacatta agctttttag ttggattcta ttttatgtta
    38581 tttggtatca cacatatcgt ctatgcattt agataagtaa aatcaaacaa ataaaaaagc
    38641 cggcttccga ttacagtttt actgttgtaa ttggaagccg gcttttttat catggacaaa
    38701 tatgcacgag gtgattttta ggtgtcgaga caaatttatg caaaaatgtg agaagctata
    38761 tacacaaatt ttatcttaat actcgaagca tagcgcagtt gtcacaacct cttttcatat
    38821 aattcattca atactggcaa atcatggact tcatattttt ctttgaatgc ttggatttct
    38881 tcttcagaat attttcttgg atctaaatgc agatcttcta ctggcaatgg gcgcttgtta
    38941 ttgatcttca catcgattac tacaggacga tctgattttg cagctgcatc aaaggcaaat
    39001 cgaagttgcg agtattctgt gactgtaaag ctatttgccc cgagcgcttc tccaacttta
    39061 ccgaaatctg catcttctaa aaatacacca aatttctttt gttccgtatc ttcttgttct
    39121 gcttcgatga aaccaaaaga atcattcgaa aagacaacat taataattgg aagctgatat
    39181 ttgacttgtg taatgatatc ttgcatgacc atagcaaacc cgccatcacc acttaaggta
    39241 aatacttgtt tttctgggta gcttagttgt gcggcaattc ctcctggcac accattaccc
    39301 atagttgcga accatccaga tgtcgtatgt ttttgtttac cgttcatatt caataaacga
    39361 atagaatgga tagtcgtgtt accgacatct gtgacgaaaa tcgcatcgtc ttcagcaata
    39421 cgattgattt ctttatagac agcctctgga cgaatcggtt cttcctctcg atcttcaaaa
    39481 gatttcaacc aatttaccca tttttcttta ttttcttgat ttgctaggta ccagctatca
    39541 gcagggcgag catcccctaa ctcgacaagt ctttgcaaag ctgtgtttgc atcaccgagg
    39601 atcgacaaag atgtttcatg gcgtcgtcca aattttgacg catcgatatc gatttggata
    39661 aattcagcag ttggattaaa aaatgcacgt ccaaatggga agtcgctacc gacaaataag
    39721 atcagatcgg cttctgccaa tgcttcattt ccgggtttag tagcgacacg tccagcaaaa
    39781 ccaagaaagt tttcataatc atcaggaata attccttttg ctagaacgga agcgacaaca
    39841 ggaactgaaa aatgttctga gaattctttg atttgcggaa atccgtttcg cgttccttga
    39901 ccaacataaa gaactggttt ttttgctttt tcaagataag gcaaagcagc tagcaaatct
    39961 ttttcttctg gtaatatcac acttgttttg tgagtgtgag cggtggagaa aggttgttcg
    40021 tcaatttctt caaaaccaag atcaacaggg atagtcacga tagccacgcc tttatgctca
    40081 taggcggctt taatggcttc atctactaca tgcggaagac tttgtggtgt catgactgta
    40141 cgattgtata cgctaacatc tgcgaaaata ggattttcat ttagttcctg gaaagagtta
    40201 tagttcatgg atgtagaagc aacttgtcct aagaacgcta ctagaggcac atggtccatc
    40261 tgtgcatcat acaaaccatt gatcaaatgt gtcgcaccag gtcctgcaga gccgaagaca
    40321 gcgccgactt tccccgttaa ttttgcatct gcagcggctg cgagtgcgcc gacttcttca
    40381 tgacgaactt gaatgtagtt gatttcggtt ttttcttgat atagcgcatc cataattgaa
    40441 ttgaaagatc ctccgggaat gccgtaaata tggtcgatac cccagctttc aagaactttt
    40501 accattgcga ttcctgcatt gatttttgac atggtgtttc actcctttag caatgatgat
    40561 acataaattt tttgtatcta gagtatagaa ctttttatcg aaaatcccaa ctaattcgtc
    40621 ttttcacaaa aatgtagtca tgtttttggg aatatgctag agtagtacat agatagattc
    40681 aagagagggg aatggcatgg ttgaatggct attgagactt gaaccttggc agcaagcact
    40741 tgctgggaca gcttttactt attttatgac agctcttggt gccgggttgg tttttttctt
    40801 taaagaaatc aaaaaagaag tgctaaactt gatgttagga tttgcttctg gtgtcatgat
    40861 tgctgcaagt ttttggtctt tgttagatcc ggctattaca aaagcagaag aaaatggaga
    40921 tattgcttgg ctagttgtaa gtattgggtt tggtttaggc ggtgtgtttt tatatatggc
    40981 agataaaaca ctgccccata tgcattttgg accacaacat gaagcagaag gattgcctac
    41041 ccatttaaaa cggacgatct tgcttgtttt ttctattaca ttgcataaca ttcctgaagg
    41101 tttagccgtg ggggtagcat tcggtgcagc agcaactgcg gataatccga cagccgctat
    41161 attagccgct gtttcggtgg cgctaggtat tggtatccaa aacttcccag aaggagcagc
    41221 tgtatcaatc ccgttgcgac aagaaggtct gagtcgtaaa aaggcctttg tttacggaca
    41281 agcttctgga atcgttgaac caattgctgg agtgattgga gcgctactag taacgaaagt
    41341 ggagctgctg cttccttatg cacttgcttt tgccgcagga gcaatgattt atgtcgtggt
    41401 ggaagaattg atccctgaag cacagcagac attgtccagc aaacgtcatt ttgcagtttt
    41461 tggcgtcatg agtggattta tcattatgat gattttggat gtggcattag gataaataaa
    41521 aaaaatatat atgatatatc aaatagggcc gcgaacttat atcgcgaccc tattttttta
    41581 gtaagttata tcataatgaa gtgaatggta tcatacatta ataatagttt tttctatgtg
    41641 tatttccttt gatccagcga acaagccaga tgataagtgc gattgggatt agaactttga
    41701 ccgcaaacca taaaagcccg cttaggacac tgacaaaaag actgatgacg aagctagctg
    41761 caattagcac taaaagaacc atgaagatat ttaagaacat atttttttga cgcatacaaa
    41821 accctcctct tgttcattct ttactataga aaaagtataa cagggattgt ttaaaaacac
    41881 ctccaactga agactgattt ttacttcatc tacttaaatg atcaaacaat tgctttttct
    41941 ctggtttatc atctaaaata gagcggaaaa ggaggagaat atcatgccta aaaatcgtct
    42001 tgaagcattt acagatgctg taatcgcgat tatcatgact attttagttt tagaacttca
    42061 tgaacctaac ggagacacgt gggaggcgct cgctgcatta ggttaccgat tttttatcta
    42121 tattgtcagt tttatcattt tagcgattta ttggaacaat catcatcatt tatttcaagt
    42181 tgtacataag ataaatggac gggtcttatg ggcgaacagt ttctttgttt ttgcgctttc
    42241 gctttttccg tttgcaactt catggatcag tgagtacatc aatagcctgg ctccagaaat
    42301 gacttatggt tttgttacct tgaccgctaa tattgcgtat tatatcttgg caaaagaact
    42361 tgtcaaagca aataaaggcc acgagaaagc attgaaggcg atgttgaata attacaagaa
    42421 atcgtatcta tcgattggtt tgaatgtgtt aggaattgtc ttagggtatt tagtagcgcc
    42481 agtagccgtg ctgatcgcaa acatcgtgat tctagctgct tggatcatcc caagtcgtac
    42541 catcgaatca caatatcggt aaaatgatcc gtgtttaaaa tttaagagaa aaatatagaa
    42601 aaaataaata aacgatcaaa tatccgagtg atactagtat tcaaagaggg atgcagatgg
    42661 atcaaaaaag aattcatgta ggactgatta ctagttttgt cacgatgctt atttatttgg
    42721 atcaaggtca tttgacttgg acagtagttg gtgtcgtagg atttttgaca ttattcacag
    42781 caataaaaaa ataaacatct ttatgtaatc tgatgttcta tctatttaag aaaagcatgt
    42841 ttacagcagt atgattctgt tgtaaacgtg tttttatttg tggaaaattt aatttttaga
    42901 aaagtgtatc gaaaaataga tagttagttt ttccacagtg gataacttcg taagagataa
    42961 aaataaaaaa ttagtcaaag aagggcgtat agatttcttt tgataggaga tatacaacag
    43021 aaatgaataa atattaacgt gttttcaaac agtataaaac ggtgtggttt ttggaataaa
    43081 aaaataacat atctcatttt acgcttttgt tattttatac ttaaaaaata acgacacaga
    43141 aaataccgtg tctcagattt ctctactact tcta
//
LOCUS       DACDKM010000014        41906 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20834_2016
            SAMN12640370-rid9891663.denovo.014, whole genome shotgun sequence.
ACCESSION   DACDKM010000014 DACDKM010000000
VERSION     DACDKM010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640370
            Sequence Read Archive: SRR10040891
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 41906)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 41906)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742835.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742835.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:04:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,012
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 29 of 75
            Pseudo Genes (incomplete)         :: 33 of 75
            Pseudo Genes (internal stop)      :: 19 of 75
            Pseudo Genes (multiple problems)  :: 5 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..41906
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640370-rid9891663.denovo.014"
                     /isolate="E20834_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            240..1850
                     /locus_tag="IV334_000741"
     CDS             240..1850
                     /locus_tag="IV334_000741"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HAP8942834.1"
                     /translation="MTKYIFVTGGVVSSIGKGIVAASLGRLLKNRGLKVTIQKFDPYI
                     NVDPGTMSPYQHGEVFVTDDGAETDLDLGHYERFIDINLNKYSNVTTGKIYSEVLRKE
                     RKGEYLGATVQVIPHITNEIKEKIMRAAKMTDSDVIITEVGGTVGDIESLPFLEALRQ
                     MKADVGTDNVMYIHTTLIPYLKAAGEMKTKPTQHSVKELRGLGIQPNILVVRTEQPVS
                     QSVKNKLAQFCDVEPEAVIESPDVDTLYSIPLVLQAQGMDQIVCDHLKLDTPEADMTE
                     WKELEERVLNLKKKVRIALVGKYVELPDAYISVVEALKHSGFAYNSDIEIDWIKAQEL
                     TRENVEERLKDADGILVPGGFGDRGVEGKIEAIRYARENDVPFLGICLGMQMACVEFA
                     RNVVGLEDASSAETNPDTANNIIDLMADQENIENLGGTLRLGLYPCKIKKGTKTAEAY
                     DGADVVQERHRHRYEFNNKYRQLFEEQGLVFSGVSPDNRLVEIVELPEKKFFVGCQFH
                     PELISRPNRPQKLIKAFVGASLADHETK"
     gene            2142..3011
                     /locus_tag="IV334_000742"
     CDS             2142..3011
                     /locus_tag="IV334_000742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphate aldolase"
                     /protein_id="HAP8942835.1"
                     /translation="MPVVSGAEFLKAARKGGYAVGGFNTNNLEWTQAILEAAEAKQAP
                     VLIQTSMGAAKYMGGYKICKDLITDLVEAMNITVPVAIHLDHGDYEAALECIELGYTS
                     VMFDGSHLPFEENLKLAKDVVEKAHAKGISVECEVGSIGGEEDGIIGNGELADIEECK
                     QMVATGIDYLACGIGNIHGQYPENWKGLAFDHLQAIADAVGPDMPLVLHGGSGIPQDQ
                     IEKAISMGISKVNVNTEFQLSFAAATRKYIEAGKDKEGKGFDPRKLLAPGKAAIVEDA
                     KSHIDWFGSANKA"
     gene            3176..4447
                     /locus_tag="IV334_000743"
     CDS             3176..4447
                     /locus_tag="IV334_000743"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HAP8942836.1"
                     /translation="MKKIVIKGNRPLAGEVTISGAKNSAVALIPAAILADSPVILEGV
                     PDIQDVHSLIEILEIMGATVHFSNNILEIDPREVVSIPMPHGKINSLRASYYFMGTLL
                     GKFGEAVVGLPGGCYLGPRPIDLHVKGFEALGAEVTNEHGAMYLRTENKGLRGNRIFM
                     DVVSVGATINVMLAAVKAKGQTVIENAAREPEIIDVATLLNNMGAKVRGAGTDVIRIE
                     GVETLHGCRHFMIPDRIEAGTYLALAAAVGNGIKVKNVIFEHLESFIAKLQEIGVHMK
                     ISEDEIEVYPSKELKPANIMTYPYPGFATDLQQPLTALLLMTTGTSEIIDTIYAKRVN
                     HVPELARMGADITVEGNMIIVNGPNKLHGTEVVASDLRAGACLVIAGLLAEGTTTIYN
                     VDYILRGYDHIIEKLTALGASIEMIEEVGAE"
     gene            4444..5739
                     /locus_tag="IV334_000744"
     CDS             4444..5739
                     /locus_tag="IV334_000744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HAP8942837.1"
                     /translation="MSDYLTMAELENKTLKEVYSYAKEFKIPYYSKMNKKELSLAVIR
                     AQAEKQGFYYMEGILDIVGQDGYGFLRPINYGPSAEDIYISASQIRRFSLRNGDKVAG
                     KARPPKESERYYGLMHVESVNGKDPEEAKERPHFPALTPLYPQRQLQLETTPQRLSTR
                     MIDIFSPIGFGQRGLIVAPPKAGKTSVLKEIANGITENYPEVELIVLLIDERPEEVTD
                     LERSVKGDVVSSTFDLQPQNHTRVSELVLERAMRLVEDKRDVVILMDSITRLARAYNL
                     VVPPSGRTLSGGIDPAALYKPKKFFGAARNIEEGGSLTILATALVDTGSRMDDVIYEE
                     FKGTGNLELQLSRELSERRIFPAIDIKKSGTRKEELLMPSEELEEIWKLRKNMTGDSL
                     EYTEQLIRFLRKTKNNQQFFKEFQDVSFGKKNPTRNLKR"
     gene            5838..6101
                     /locus_tag="IV334_000745"
     CDS             5838..6101
                     /locus_tag="IV334_000745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
                     /protein_id="HAP8942838.1"
                     /translation="MKENIHPDYHPVVFMDSTTGFKFLSGSTKTSQETVEWEDGNTYP
                     VIRVEVTSDSHPFYTGRQKFTQADGRVDRFNKKYGLKDANAAE"
     gene            6546..6995
                     /locus_tag="IV334_000746"
     CDS             6546..6995
                     /locus_tag="IV334_000746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAP8942839.1"
                     /translation="MRFDQLIAEELIVFDPSIDSKGSLFERIGDVLKKTNKVRNVQKV
                     VKALFKREEEISTGIEDGFGIPHAKSKYVTKPALCFFHTAKLTDYSGIDEMPIEYIFA
                     IVVPEKSADMHLEILSTLSRRLMNADFRKGIKEATTPADIIRQISQE"
     gene            7052..8470
                     /locus_tag="IV334_000747"
     CDS             7052..8470
                     /locus_tag="IV334_000747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP8942840.1"
                     /translation="MKIVGVTACPTGIAHTYMSAEKLEVTARNLNHQAKFETQGVKTE
                     NQLSEQEIKEADAIILAVDKEIELDRFAGKKVKRVSTSRAIKEPQVVIDEALRDIGVF
                     VVSNEKEPAANEKKPTIYNHFMNGVNYMLPFVIAGGIIIALSFAFGITAADPNSADYN
                     VLAAAFSRIGGDTAFAMMVPALGAGIATSIAGKAGFAPGIVAGLLASTGGSGFLGGMI
                     GGVLAGYICDFLANKISVKKEFSAIYQLIVVPFLSILIIGLLMVFVIEQPITWVLDAL
                     TNWLNSLGNTSGLLFGLLIGIMMAADMGGPINKSTSTFSIGLMSAGVNAPIAACMAAG
                     MTPPLGIALATLLFKHKFTKEERTSGQSCWILGASYITEGAIPFAVSDPIRVIPSLMT
                     GSAVAAGISMAVGVTSMAPHGGIWVMLIPNVMNNLPMYVLAIASGTVTTAFMLGLLKK
                     TVKEKKQDKWTKNENLTNEVEA"
     gene            8470..9330
                     /locus_tag="IV334_000748"
     CDS             8470..9330
                     /locus_tag="IV334_000748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketose-bisphosphate aldolase"
                     /protein_id="HAP8942841.1"
                     /translation="MLYTMKELLKVAKENQFAVPAFNICSYDMMKAIIEEAERLEAPV
                     ILEIHPDEIAYLSDEFVAAVRAYAYTSKLPIVIHLDHGGTIEDVLRAIRNGYSSVMID
                     ASLKSFDDNVQITKKVVALAHAVNVSVEAELGTIGNNGSAEGGAETIIYTDPDQAKIF
                     VKETGIDTLAVAIGTAHGLYPKNRTPKLNIDLLKELNQQIDLPFVLHGGSGNPDAEVS
                     EAVKYGVAKVNLSSDLKSVFFNALREILSENPSMYEPNAIYPAANKKVQEVVKHKMEI
                     LNTVGKASLY"
     gene            9504..10277
                     /locus_tag="IV334_000749"
     CDS             9504..10277
                     /locus_tag="IV334_000749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HAP8942842.1"
                     /translation="MLNEVENRQQEIVNYLKNNQFARVTDLIELVNYSEATIKRDLVF
                     LEKSGLIRRVRGGAMLIDNRKIDVPYLMKITKLEEETEKRKIADTASSLIRDDMVLFL
                     DSSTTSLHLVRNLARFDGLQIITNGIVTAAMLSEYTTAKVSVLGGAIVPKRATINGSK
                     AYNDALSYYADLAIMSCRGLDFSQGVTETHEGEAMVKKAFRKQSDRLAVLVTQQKIAH
                     KFMHQAIAFHEIDYLVTSKELTKEQYAKVKEHHIECLYG"
     gene            10424..10789
                     /locus_tag="IV334_000750"
     CDS             10424..10789
                     /locus_tag="IV334_000750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2512 family protein"
                     /protein_id="HAP8942843.1"
                     /translation="MKHVKALVVKAIMIWAILWVVLTGLYGVSFMDSTIVGVIIVVMI
                     YVLGDLLILRKVGNIAATIADAGSAAVILWLYLYFMNDTVDIWMKVLIPALLIGVAEW
                     FFHKWLLSQGIVPDERKME"
     gene            complement(10937..12145)
                     /locus_tag="IV334_000751"
     CDS             complement(10937..12145)
                     /locus_tag="IV334_000751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP8942844.1"
                     /translation="MEKKLFNKYFIGITLINFIVYLIYYLLMVIMAVIAQDTLHASLS
                     QAGFASGIYIIGTLLARLFMGKQLELFGRKFTLRGGAIFYLLSTLAYLYTPTIGALYL
                     VRFLNGFGYGTVSTATNAIVTAYIPASKKGEGINYYGLSTSLAAAVGPFLGMILLNMT
                     DFRFIIWFSIVLVFFVTIACLVFPVKNIPLSEEQKISLRSWNINSFIEKKALFITGIA
                     FLMGLSYSSVLSFLSSYTKVIHLVAAGSFFFVVYALVITFTRPLTGRIFDVKGEQYVM
                     YPSYLFLTAGLFLLSVTTNSVTLLISGALVGLGYGTFMSNGQAVCLKIVREHRISIAL
                     STYFIGLDLGLGVGPYLMGMLKSLTSFRGLYVIAGVIPLICTALYLVNGRKHSHEAEG
                     ELIESLKNEI"
     gene            complement(12164..12442)
                     /locus_tag="IV334_000752"
     CDS             complement(12164..12442)
                     /locus_tag="IV334_000752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8942845.1"
                     /translation="MNYWMNTIINRLETAYQTRFDMKASLVFLNDAYQNSIELIKAVD
                     ENPTNECEEFLNLFMSTRDLFIRQLVDRYPSNYHDVEVQIQKLKAYSA"
     gene            12635..13291
                     /locus_tag="IV334_000753"
     CDS             12635..13291
                     /locus_tag="IV334_000753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HAP8942846.1"
                     /translation="MDSRVLKEYLDKNDFPIIRKGYHKYLTFEGMEDSHTYILKEGTV
                     KASVISNDGREFNLRYIKDLEIVSLLKDEYSQFIDSPYNIRVESEYAEFYRINRVRFW
                     QNINQDPKLQHYVKEYYRYRLLYSMKKMQQMLLNGKLGAVCTQLYELYDLFGVRTEEG
                     MLIDFSITNEEIAKFCGISTASSVNRILKQLKKDGIITVKDHHIVILNMDLLEDNIIF
                     "
     gene            complement(13733..14530)
                     /locus_tag="IV334_000754"
     CDS             complement(13733..14530)
                     /locus_tag="IV334_000754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HAP8942847.1"
                     /translation="MMKKFFLFFLLLVSVGWPVATHADTATVNDEAGLFTTDQIQSLE
                     QQAEKLNEKIKGRVLIVTTTSNSEEPRDFADNYLRDAVGNDQNGSVLLLDMGQREIYI
                     STSGNMIDYLTDSRIDSILDDVYDQMTNAAYYNAAKAYLSKSATYVEDGVPGGHYRVD
                     EETGKITRYKVLTTVEIIIAVVLALILSLVFYFVTVSRYQLKSGIYKYPFREKSSIKL
                     TEKTDRLTNSFVTTRRIPKSPPPGSGGGGSTTHSSGGGTFGGGGRSF"
     gene            complement(14527..15711)
                     /locus_tag="IV334_000755"
     CDS             complement(14527..15711)
                     /locus_tag="IV334_000755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TFIIB-type zinc ribbon-containing protein"
                     /protein_id="HAP8942848.1"
                     /translation="MDVLTHKCPNCGGPLTFDPKDQKFHCEYCLNIYTEEEVSQYEQK
                     QKEARMAGEESKEEPSPTEDFTFTAQEQLADMNEAERKAVDEAGGFSDTADESADNTA
                     AIDLFLCPNCGAEIVTDATTAATYCYYCHNPVVLSGRLSGEFLPNKVLPFAVEKEEAI
                     NRFLAWTKKKWFVPKAFFNKDQIDKLTGVYFPYWATDAEVDGAMQANGTVIRIWRIGD
                     IEYTETKQFAVSRAGKLSFKELVKNALSKNTQQKMVEGVQPFPLDKAIDFKSQYLAGF
                     QAEKRDIEYEAIKSQIQSELKDYSEKLLKDTANGYTTLTGVRTSASITNEVNNYVLLP
                     VWLVTYRSNDSSKKVYYYAMNGQTGKVSGVLPISQKKLGLTALGIFAGLAILFLIGGY
                     LI"
     gene            complement(15736..16983)
                     /locus_tag="IV334_000756"
     CDS             complement(15736..16983)
                     /locus_tag="IV334_000756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virion core protein"
                     /protein_id="HAP8942849.1"
                     /translation="MGLIKAATSMVGGGLADQWLEVIEPDNMSDTTVMTKGVKVRKDD
                     KRGSNRKGTEDVLTDGTVVHVYPNMMMLLVDGGKIIDYTAEEGYYTIKNDAAPSMFNG
                     TLKEAIAETFDRFKFGGVTPQKQQVFYINLQEIKGIKFGTSAPLNYFDNFYNAELFLR
                     AHGTYSIHVVDPILFYTNAIPKNKTQVEINDINEQYLAEFLTALQSAINQMSADGQRI
                     SYVPSKSLELSKYMDTALDDSWRELRGMEIVSVAVASISYTDDSVKLINMRNEGAMLG
                     DPSVREGYVQGSIARGMEAAGKNEAGAMTGFMGVGMGMNANGSYLSQAAQNNQEQIKQ
                     QAEKQNQQTAQGNADTWTCPVCGTENSGKFCSNCGAAKPVENVQPKLQMRCSECNEIV
                     DLSNGIPKFCPNCGKPFKGIPVN"
     gene            17198..18943
                     /gene="dnaX"
                     /locus_tag="IV334_000757"
     CDS             17198..18943
                     /gene="dnaX"
                     /locus_tag="IV334_000757"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HAP8942850.1"
                     /translation="MAYQALYRVWRSQRFEDIVGQKAVTQTLKNAIVSHKTSHAYLFT
                     GPRGTGKTSAAKIFAKAINCPNSKDGEPCNECEMCRSITAGTQEDVIEIDAASNNGVE
                     EIRFIRDRANYAPTKAAYKVYIIDEVHMLSTGAFNALLKTLEEPKESVIFILATTEPH
                     KIPATIISRTQRFDFKRINTADIVEHLEFILKESTISYEPAALNVIARSAEGGMRDAL
                     SILDQAISFSEGTITLDDAMQVTGSLTDEMMDSYLSACVKKEVPEALETLNQILAAGK
                     ESRRFLEDMLQYCRDLLMYQQAPNLVEEQSSTIRSTFTELAQTVSADQLFSMIKILSD
                     TQNEIRFTNSAAIYLEVATVKLAQLPSASVPSADVAFADDQIVKQLQQEIKELRNEIK
                     ELRSNTGIENTKVQPVQQTNHKKQTSTYRVPTERVYKVLREATRQHLNQVKEVWEDLL
                     MSLSVTQRAMLKASEPVAAGPTGLVIAFDYEIVCQRAANDEELQLSVHNNLSRMIKDY
                     APDPVYITRESWPELRRNYLTQAQEEGTENSFASEDSEGEIELLPQEQEVPEETLVVK
                     AEELFGSEAVTVVDD"
     gene            18963..19277
                     /locus_tag="IV334_000758"
     CDS             18963..19277
                     /locus_tag="IV334_000758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HAP8942851.1"
                     /translation="MMRGMGNMQGMMKQVQKMQKEMAEAQELLNKTEFTGKATNELVK
                     AVFTGDRRMKDIQINEEVVDPDDIEMLQDLVVMAVNDAIAQIDVETEKKLGKYAKGLP
                     GF"
     gene            19302..19898
                     /gene="recR"
                     /locus_tag="IV334_000759"
     CDS             19302..19898
                     /gene="recR"
                     /locus_tag="IV334_000759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein RecR"
                     /protein_id="HAP8942852.1"
                     /translation="MQYPEPIAKLIDSYMKLPGIGQKTATRLAFYTIDMKDEVVNEFA
                     KSLLSVKRDLHFCSICGNITEEDPCEICRNPSRDRSVILVVEEPKDVMAMEKMREYHG
                     LYHVLHGVLSPMEGTGPEDINISSLLQRLHDADVKEVIIATNATTEGEATAMYLSRLI
                     KPAGIKVTRLAHGLSVGSDIEYADEVTLLKAVEGRREL"
     gene            19962..20606
                     /locus_tag="IV334_000760"
     CDS             19962..20606
                     /locus_tag="IV334_000760"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HAP8942853.1"
                     /translation="MNGLFITIEGPDGAGKTSILNELYPLLKEVAKTEIIQTREPGGI
                     PIAEEIRAVILDPKNDRMDERTEALLYAAARRQHLVEKVLPALAKGKIVLCDRFVDSS
                     LAYQGAGRRIGVTEIARLNEFATEGTTPDFTLYLDVDSDTGLRRIKKNRQNQIDRLDS
                     EGLEFHQRVRHAYLKLAEENPERIHKVDARKSFEEVLQTSYHTIIEQYPQFFEN"
     gene            20621..20950
                     /locus_tag="IV334_000761"
     CDS             20621..20950
                     /locus_tag="IV334_000761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8942854.1"
                     /translation="MKIILAIIQDKDSNRLSNELIDANIRATKLSSTGGFLKAGNSTF
                     IVGIEDDRVEEALEIIKKTCESRKQFVSTPVTLDISMDGGVPYPVEVEVGGATVFVLP
                     VEGFHQF"
     gene            20964..21902
                     /gene="holB"
                     /locus_tag="IV334_000762"
     CDS             20964..21902
                     /gene="holB"
                     /locus_tag="IV334_000762"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HAP8942855.1"
                     /translation="MNQEINLAQMQPIVYQQLQRSFEHGRIAHAYLFEGEKGTGKHEV
                     GIWLAQHLFCTQMKDNLPCGKCNNCQRIEKKEHPDVLVIEPEGQTIKVDQIRRLQTEF
                     SRSGYESRKKVFLIKEAEKMNSSAANSLLKFLEEPPGDFLAVLETDAVGRILPTIQSR
                     CQVLHFQELSKEALIHRLQEEQIPLEKAKLLAFLTNSLGKAVEISQDEWFNDAKDLIY
                     QWFVYLQKQDTQAFIYVQKKLVKSFKEKTQQFTGLSVLMFYYQEAQRQAVANGEYGKV
                     KQINQIIERILIAEQKLRSNVSFQAVAEQFVLHTIF"
     gene            21938..22762
                     /locus_tag="IV334_000763"
     CDS             21938..22762
                     /locus_tag="IV334_000763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage 0 sporulation family protein"
                     /protein_id="HAP8942856.1"
                     /translation="MVEVVGVRYRDAGHIYYFAPGDSEYVYSDKVLVESQQSLHIATV
                     VLPKKEIDPNDLPEAIRPILHKASQAELEKDKKNKKDAYDALKIAKEKVQLRELEMKM
                     VKAEYTFDRNKLIFSFTADGRIDFRELVKDLAAVFRTRIELRQIGVRDEAKIIGGIGP
                     CGRPLCCSSFLGDFVPVSIKMAKDQSLSLNPTKISGLCGRLMCCLQYESEEYETAKKE
                     LPDFGKVIATPDGKGKVVGLNLLSRIVSIRLTGRENPVEYEWDELKEFIQVGEMNG"
     gene            22755..23102
                     /locus_tag="IV334_000764"
     CDS             22755..23102
                     /locus_tag="IV334_000764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication initiation control protein YabA"
                     /protein_id="HAP8942857.1"
                     /translation="MDKRSLYDGLNQLESELRNTLTELVEMKHALHEIVEKNTTLEME
                     NQRLREHLRELNQVAQAPAENVKQELSKSRMNLEKIYEEGFHVCYDLYGSRRDNDEPC
                     AFCLEVIYRESGR"
     gene            23171..24043
                     /gene="rsmI"
                     /locus_tag="IV334_000765"
     CDS             23171..24043
                     /gene="rsmI"
                     /locus_tag="IV334_000765"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HAP8942858.1"
                     /translation="MYNQKSFKGNPVYGDLYLVPTPIGNLEDMSYRSVRMLQESDLIA
                     SEDTRNTQKLLNHFEIHTPQKSLHEHNYKERVPQLIALLKEGKTIAQVSDAGMPSISD
                     PGHELVLACIKEGISVISIPGPTAGMTALIASGLVPQPFLFYGFLPRKKKEQKETLKK
                     LKEQTVTLIFYESPYRITATLTNFLEIFGNRQVVLCRELTKVHEEYLRGSTEELLAYI
                     EEHPVKGECCLLVEGNLFEMPVTEVDEELGTLKEQVEAKIASGEKPNAAIKAVALKNG
                     LKKQEVYRQYHELD"
     gene            24152..25273
                     /gene="dinB"
                     /locus_tag="IV334_000766"
     CDS             24152..25273
                     /gene="dinB"
                     /locus_tag="IV334_000766"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HAP8942859.1"
                     /translation="MMELQFPLHNDTSRKIIHIDMDAFFASVEERDHPEFRGHPLVIA
                     RHPSDTGGRGVVTTANYEARKFGIHSAMSAQKAYELCPQAIFKPGDHHKYSDISKQVR
                     EVFYRYTDIVEPVSIDEAYLDVTENKINCKSAIKIARMIQADIWESVQLTCSAGVSYN
                     KFLAKLASDYQKPRGITVVAPQDAVPFLKALPIEKFHGVGKKTVPRMHELGIYTGKDL
                     YECTEMMLIRNFGKMGYSLYRKVRGIHDSPVNVTRERKSVGKEHTYGQPLQTEEAVLT
                     QLRQLAEKVEEALRRVQKHGKTVVLKVRYTDYSTVTKRVTLPEYIYKKEALFYQASLI
                     WEEILGVEKGIRLLGITLTNLDPMTYENIVLPLWENQEI"
     gene            complement(25327..25929)
                     /gene="nrdG"
                     /locus_tag="IV334_000767"
     CDS             complement(25327..25929)
                     /gene="nrdG"
                     /locus_tag="IV334_000767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase
                     activating protein"
                     /protein_id="HAP8942860.1"
                     /translation="MRNPKPKEWLAKDYSQNYIADYKPFNFVDGEGVRNSLYVSGCLF
                     ACEGCFNKAAQNFRYGKPFTPELEEQIIADLRNDYVQGLTLLGGEPFLNTDVCLQVVR
                     RIRKEFGTTKDIWSWTGYAFDELLQDSEDKLELLSQIDILVDGRFELSKRDLKLQFRG
                     SSNQRIIDVQKSLESNQVVIWEKCTDATETYEQIKKQDLI"
     gene            complement(26244..28433)
                     /gene="nrdD"
                     /locus_tag="IV334_000768"
     CDS             complement(26244..28433)
                     /gene="nrdD"
                     /locus_tag="IV334_000768"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HAP8942861.1"
                     /translation="MKVNYEEKTVVTQQPETSQMKVIKRDGRHVDFDEQKINEALIKA
                     EKKIHGSLSPLTYEKIQMIADLVVQEIKSRFAENVKIYEIQNIVEHILLEKNEYELAE
                     EYINYRTKRDFARSKATDINFSIEKLINKDQTVVNENANKDSNVFNTQRDLTAGIVGK
                     SIGLKMLPPHIANAHQKGDIHYHDLDYHPYTPMTNCCLIDFKGMLNNGFKIGNAEVES
                     PKSIQTATAQISQIIANVASSQYGGCSADRTDELLAPFAELNYKKHLKDAEEWIDSPE
                     RQKEYAKAKTKKDIFDAMQSLEYEINTLFTSNGQTPFTSLGFGLGENWFEREIQKAIL
                     QIRINGLGSEKRTAIFPKLIFTLKKGVNLNPEDPNYDIKQLALECATKRMYPDILNYD
                     KIVELTGSFKVPMGCRSFLQGWKDENGQEVNVGRMNLGVVTLNLPRIAIESKGDQNKF
                     WQLLSDRLEIMKDALLYRVERCKEAIPANAPILYMYGSFGKRLSRTDSVNELFKNRRA
                     TVSLGYIGLYEVASAFFGGEWETNPEAKAFTLDIVKELKANADAWGDEYGYHFSVYST
                     PSESLTDRFCRLDTEKFGIIENITDKEYYTNSFHYDVRKNPTPFEKLEFEKDYPKYCS
                     GGFIHYCEYPMLQQNPKALEAVWDFAYDKIGYLGTNTPIDHCYQCGFEGDFKPTERGF
                     ECPECGNHDPKTCDVVKRTCGYLGNPQARPMVHGRHKEISSRVKHMK"
     gene            28759..29526
                     /locus_tag="IV334_000769"
     CDS             28759..29526
                     /locus_tag="IV334_000769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP8942862.1"
                     /translation="MEKMIEVKHLSKSYGSKLSPVKALDNISFTVEEGEFVGIMGPSG
                     AGKTTLMNILSTINLPTMGSVSIQGKEITKMKNSELSDFRRKKLGFIFQEFNLIDTLN
                     AKDNILLPLAVERMTKEEMEKRVRHVAQLLNIEELLKRYPDELSVGQRQRIAAARALV
                     VQPQVIFADEPTGSLDSKSATELLNYLKTMNQKEKATILLVTHDPYTASYCDRILFIK
                     DGVIFSEVVRRGTRREFFEKVIDMQATIGGGGKMNAV"
     gene            29516..31558
                     /locus_tag="IV334_000770"
     CDS             29516..31558
                     /locus_tag="IV334_000770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP8942863.1"
                     /translation="MLYKLAVKSFFRQSRGYLVYFFSLTLSVMIYYSFSAMTYDQSLV
                     RRASQDTSIDGGLSFGGFIITVVLLFFVFSANRFFLNRRQKEIGIYQLFGMNKLQISG
                     IYVLEIMIIGLFACISGILLGIIFSKLFSMILVRMMDMDLTSPFFISIPSVVDTLFAF
                     FIILLAVSVYSIWKIWRYPMIRSFGEKEQADSSTMRFRTRHRLLAVIGLLLLTSGYFG
                     ASHFREITSWMISYHYLDTLVFFFPFLIFFVCVVGTYLFFCYTFRLFLIILSKWKIYY
                     YGINFLIMGNTQVHLLKSWRTNSLITVILGISLMTIGGTASISTILSYQEKVSSPMDY
                     QFDVETEKKIKPILAEEGQKITKEITLHYKVIGSIYSQNLEEPMFQTANLITETQYQA
                     FRKVNPDLPNISLKSPGHTVLLDQMQTLFRNISLYGSSIGLPDNQELKIQQMLPSVLG
                     DETMVYLSPVLIVDDTVFEKAKGVDYQVVNVDVSGGNNEKIDERLSNDLSIKWLNAVY
                     YSYDIVNGSLQGEISTKKLPDDQMKEAQFSQESSRLNFSSSYSKLRAVNRRIGINIFV
                     TLFVGMIVIIATGSILTVRQLAEAEEEKENYHLLTKLGIPQRRIRRMIFEQNALTFFP
                     PMILGITHAVFAINVFTQYIKTADYWLAYLVSGLLILIYLLLYVITSQLYCRIIEE"
     gene            31839..31988
                     /locus_tag="IV334_000771"
     CDS             31839..31988
                     /locus_tag="IV334_000771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="teichoic acid D-Ala incorporation-associated
                     protein DltX"
                     /protein_id="HAP8942864.1"
                     /translation="MNKWRSNPKLNYWGKFIIRTIFYSAVLLFLIYLYHYKNIQGGTF
                     IYNEF"
     gene            31999..33513
                     /gene="dltA"
                     /locus_tag="IV334_000772"
     CDS             31999..33513
                     /gene="dltA"
                     /locus_tag="IV334_000772"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltA"
                     /protein_id="HAP8942865.1"
                     /translation="MEIKTIIEAIDDWGINEPDRVAYQADETHTFGELKQASDALAYY
                     LEKKVEGDGPIVVFGNLEFEMIVSFLGVVKSGHAYLPIEEHTPKERILSIFRVAKPSM
                     VISIGDWIEDIPSVPVITKKEFQEIKKIPVGFYPRNSVKGTENFYIIFTSGTTGEPKG
                     VQISHDNLVSFVQWTMEDFGIKPGMHFLAQAPFSFDLSVFSIYPALVSGGMLKPLNKA
                     VVQDFRQLFATLPALKLNVWVSTPSFMDICLMEPTFNGENVPELDMFLFCGEELTKKT
                     AESLLDRFPNARIYNTYGPTEATVAISSIQIDQHVLDSYDRLPIGYVKKDTKVSIVQE
                     GKPVSKGETGEIIIAGPSVSKGYLNNPEKTAAAFFCQNDLASYHTGDAGRLDEYGLLF
                     YEGRMDQQVKLHGYRIELGDIEHYLLQDNRIKQAVVVPKYQGTKVQQLVAFVVLENKT
                     KQPDFQLTKSIKEQLLEVVMDYMIPQKFHYVDLLPQTVNGKIDRKKLTAEVNPE"
     gene            33510..34715
                     /gene="dltB"
                     /locus_tag="IV334_000773"
     CDS             33510..34715
                     /gene="dltB"
                     /locus_tag="IV334_000773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltB"
                     /protein_id="HAP8942866.1"
                     /translation="MMLPHMIPYAEPFYFFLLAIALVPIVLSLLIWEKRLPVYQSAVT
                     LFFLFVSFGGNYWQQGAALIGYVLWQTILVWGYFVYRQRKNQTGWFFAAVILAIIPLF
                     LTKVTPFFEHGKASLLGFLGISYLTFKSVQMIMEIRDGMIKTYKPFRYIQFLLFFPTI
                     SSGPIDRYRRFEKDQLHPPVRDKYVDLLGKGVQNIFVGFLYKFIIGYYFGQVLLPVVG
                     RIALQHGGISWALVGYMYVYSMYLFFDFAGYSLFAVGTSYMMGYQTPINFNKPFLSWN
                     IKEFWNRWHMTLSFWFRDYVYMRLMFFMMKKKFFKSKIVMSNIGYFALFLLMGIWHGL
                     TWYYIVYGLYHACLICLTDAWIRFKKKHKEQLPSNKFTHGLAVFLTFQAVCFSFLIFS
                     GFLDKLFFS"
     gene            34760..34993
                     /gene="dltC"
                     /locus_tag="IV334_000774"
     CDS             34760..34993
                     /gene="dltC"
                     /locus_tag="IV334_000774"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltC"
                     /protein_id="HAP8942867.1"
                     /translation="MEEQILSILEEITGTDEVRKNRDIDLFDEGLLDSLASVQLLVEL
                     DGELDIQVPVSEFEREDWSTPNKIIQQATALKG"
     gene            34996..36264
                     /gene="dltD"
                     /locus_tag="IV334_000775"
     CDS             34996..36264
                     /gene="dltD"
                     /locus_tag="IV334_000775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltD"
                     /protein_id="HAP8942868.1"
                     /translation="MKKKLFAIFGPVVLAVGLLFVFFTSSYQVDISNPETIRRASSSM
                     SVNVLKGDAIKNYALSEKQYIPFFGSSELSRISPFHPSVLAEKYQRNYRPFLLGAPGT
                     QSLSQYMMMRSAGDAMKNKKVVFIISPQWFVKNGVKTDYFNTYYSELQTYDWLFSMKK
                     VTPADRYLARRLLTFSKVKENDTLTAILQTIKKGKLPLPESLNQLRSQWNMLKREDEV
                     FSNIGLKDRQQKIDHESKRLPKQYQETELSILANQIGERETTNNPFGLKNDFYTHRIR
                     AHEPELKQSQKNWDYRFSPEFSDFQLVLDQLAKNHNEVLFIIPPVNEKWSDYTGLSQE
                     MLQGFAKKIKFQLNSQGFNRIADFVNQAGTNYFMEDTIHLGWKGWLAADQQIRPFLEE
                     NHITASKYHLDDAFFSKSWQHQIPDKLQLK"
     gene            36405..37487
                     /locus_tag="IV334_000776"
     CDS             36405..37487
                     /locus_tag="IV334_000776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family metallopeptidase"
                     /protein_id="HAP8942869.1"
                     /translation="MDTKEEQLLIDLTSAKGVPGNEEEVREVFREYAKPFADDIFYDG
                     LGSVIAKHGAGGGPKVFISGHMDEVGFMVTKITEKGFLEFQTLGGWWGQVMLAQQVEI
                     KTREGKIVHGVIGSKPPHVLTPQVRNKPYEIKDMFIDIGASSQEEAKEWGIRPGDMVT
                     PYIEYKRMNGSKYLLAKAWDNRIGTAVSLRVLENLSKEAHPNVLFAGSDVQEEVGLRG
                     ARTSTHLVNPDIAFALDTGTAGDTPGMTPKEADSILGKGPQILIFDASMIPHKKLLNF
                     VIDVAEEMDIPFQYTVISGGGTDAGQMHLTRDGVPSVAITVPVRYLHSHTSVIHEDDY
                     LNTVKLVTELVKRLDSETVEKIRSYE"
     gene            37487..38290
                     /locus_tag="IV334_000777"
     CDS             37487..38290
                     /locus_tag="IV334_000777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HAP8942870.1"
                     /translation="MNNRIPISEYRKKARVSLENQWGINTWLVFLSIIFTSVIQNFFD
                     GFSPFPEESMQQHLLSFLVDNLLLFAFNYALFYIALVVVRGGRPKSNLLFSVFQRKYF
                     GSILGINLINSVINWLLNSLIFLPMFLISGVSTYMQLLFSSNAVPTGVMPEDFLDISF
                     VLSMIGMIFVSALIMSIVGGLFQFAAWTKFDYPDLSVIQCLKYGWFLLKDRIGTYILL
                     QLSFIGWYLLGAIALFIGLLWVIVYVNVSVAEFYEQARIEKGGPDAYFT"
     gene            38360..39100
                     /locus_tag="IV334_000778"
     CDS             38360..39100
                     /locus_tag="IV334_000778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HAP8942871.1"
                     /translation="MKLTVLGCLGAYPYKNQGTTGYLLQSDGFNLLIDAGSATLIKLQ
                     EHLDPLDLDAVILSHYHHDHIADLGVLQYYWQLHPKRNPETLLSIYGHTKDSIHFEEL
                     TLENVSKGHPYFEAEELKIGPFLITFMETIHPVVCYAMRIVEETTGKVFVFTGDSGYL
                     ESFADFAKDADLFLADTYLFEGNEHHHAHFTSKEAGELAKSANVKKLVLTHLPQFGDL
                     EQLKNEAQKAAGKGIDIELAAVDKVFNI"
     gene            39111..40115
                     /locus_tag="IV334_000779"
     CDS             39111..40115
                     /locus_tag="IV334_000779"
                     /EC_number="6.3.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase"
                     /protein_id="HAP8942872.1"
                     /translation="MIFVPNENHDPRINLAIEQYLLQEMPLDEPILLFYINEPSIIIG
                     RNQNTLEEINRDYVEENGIHVVRRLSGGGAVYHDFGNLNFSFIMPDDGDSFRDFAKVT
                     QPIIQALHELGVAGAELKGRNDLVIDGMKFSGNAMYATNGRMFAHGTIMFDSDINEVV
                     NALKVKKDKIESKGIKSIRSRVTNIKPFLPEEKQKMTTEEFRQEILLKIFGVESVEEV
                     KTYELTDEDWKKINRISEEYYRNWDWNYGKSPDFNFSRQKRFSIGSIEVHLNVSEGMI
                     KEAKIFGDFFGLGDISDVEKKLVGQKYDKASLSKIVSQIDVKKYFGAIEPEELLTLLY
                     "
     gene            complement(40160..41857)
                     /locus_tag="IV334_000780"
     CDS             complement(40160..41857)
                     /locus_tag="IV334_000780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8942873.1"
                     /translation="MKEIKDSWVFYTKDDPDFKFDSKYTRTIPTVDKDETFIMETTLE
                     KPLDEANLLIKGNHQWITVYLGEKILYQRKDYQEESNPGLSLAVIDLPANYIGKKLTL
                     TVSSPYQNYAGLPPKVYLGTTGPLISFIYSQSVPQVLTMIIAVFIAIGTFFYTIYLMY
                     KQKRLEYSLILLSCFALALGFEAVSEDILSGILFEPFVHSFLSHLFIILTSAFIICYY
                     WSRMFYYKKWYGIFCTFQLLIFIGVLLYATFTPAELPELMPIANIASVISTLVTSLAC
                     IGEAYKNNRFYVACTPWIVLVAIIHCLIYIQTALGIYQTTINWSTILYIIILIVIISY
                     NLIDHILNTDKDRRQVDFLKIKNDLLEQHYEQLRQHIQEVNTLRLEFVQEMENLQDLM
                     HTDQTKAKNYVEKILEEAKNFEMLCSFCNHQVTNLILARYQKLAAKRNIQITFQADLP
                     EELPINDDDLAQLLIHALEHSFRETHAIENPLYRKIYLSIHEQEEHFVICCEHSAHYD
                     TNLFSRGITEELDDQERFDLMMMKDVADRYTGTLTQEKDTFIDRITVQLSRNYPNPV"
BASE COUNT        13469 a         7356 c         8717 g        12364 t
ORIGIN      
        1 aaaggactta agaaataatt tctcaaagga tatcgaaaaa gaaaaaattg taagcggata
       61 gtattcattt gagtacttga ctttccctta gtcagcttgt attatacacc ttgggcaccc
      121 ttttagaggg tcagaagttt acagaaattt cggctcccta ttcttattga agaataggga
      181 gctttgttta ttgtctgaga cttttcctgc acataaatta aaaaggagtt tttgaactta
      241 tgacaaaata tatttttgtg accggcggtg tcgtttcatc aatcggaaaa gggattgttg
      301 cagcttcttt aggacgctta ttgaaaaacc gcggcttaaa agtaactatc caaaaatttg
      361 atccatacat caacgtggat ccggggacga tgagcccata tcagcacgga gaagtattcg
      421 taacagatga tggtgctgaa acagatttgg atctgggcca ctacgaacgt tttattgaca
      481 tcaatttgaa caaatactcc aacgtgacga caggaaaaat ctattctgaa gttttgcgta
      541 aagaacgtaa gggcgaatac ttgggagcaa ctgttcaagt tattcctcat attacaaatg
      601 agatcaaaga aaaaatcatg cgagcagcta aaatgactga ttcagatgtg atcattacag
      661 aagtaggggg aacagtaggg gatatagaat ctttgccatt ccttgaagca cttcgtcaaa
      721 tgaaagcaga tgtcggtaca gataatgtta tgtatatcca tacaacattg attccttact
      781 tgaaagctgc tggagaaatg aaaactaaac caacgcagca cagtgtaaaa gagctacgtg
      841 gtttaggtat ccaaccgaat attttggtgg tacgtacaga gcaacctgtt tctcaaagcg
      901 tgaaaaacaa attggcacaa ttttgtgatg tcgaaccaga agcagttatc gagtcgccag
      961 atgtagatac actttactcc atcccattag ttttacaagc acaaggaatg gatcaaattg
     1021 tttgcgacca tttaaaacta gatacaccag aagcagacat gacagaatgg aaagaattag
     1081 aagaacgtgt cttgaacttg aagaaaaaag tacggattgc gcttgtcggt aaatatgtcg
     1141 agttaccaga tgcatatatc tcagtagttg aagcattgaa acattctggt ttcgcctata
     1201 attcagacat cgagatcgat tggatcaaag cacaagaatt aacgagagaa aatgtagaag
     1261 aacgtttgaa agacgcagat ggaatcttgg ttcctggtgg atttggtgat cgcggagtag
     1321 aagggaaaat cgaagcgatt cgttatgctc gcgaaaatga cgttccgttt ttaggtattt
     1381 gtcttggtat gcagatggct tgtgtcgaat ttgcacgtaa cgtcgttgga ttagaagatg
     1441 cgtcatcagc tgaaacgaat ccagatacag caaacaatat tattgattta atggctgatc
     1501 aggaaaacat cgagaatttg ggtggaactt tacgccttgg attataccca tgtaaaatca
     1561 aaaaaggaac gaagacggct gaagcatatg atggagccga tgttgtgcaa gaacgtcacc
     1621 gtcaccgcta tgagttcaat aacaaatatc gtcaattgtt tgaagaacaa gggctagtct
     1681 tttcaggtgt ctcaccagat aaccgtttag tagaaatcgt agagctgcca gagaaaaaat
     1741 tctttgtggg atgccaattc cacccagaat tgatttctcg tccaaatcgc ccgcaaaaat
     1801 taatcaaagc atttgtcgga gcatcattag cagatcacga aacaaaataa tttttgaact
     1861 tgatagaaag agaaaagtag caagagaagg ggataagcaa ttttcttgtc acttttctct
     1921 ttttctgatt tgttattatt tgtaggaaaa aagatagaaa gcaaaagact tttttcgatt
     1981 aaagcgctat tggatatttt attttttgca aaaaacttct tgaaaatttt cattttcatt
     2041 gattttagta gaaaactacc gaaatgtttg gtaaaatagg gtcgttggtt aagaattgtc
     2101 ttaacaaatt tagttttaca aaacttagga ggaattttat tatgccagta gtatcaggag
     2161 ctgaattttt aaaagctgct cgtaaaggcg gttacgctgt cggcggattc aacacaaaca
     2221 acttggaatg gactcaagca atccttgaag cggctgaagc aaaacaagca ccagtactta
     2281 tccaaacatc aatgggtgca gctaaataca tgggtggtta caaaatctgt aaagatttaa
     2341 tcactgattt agttgaagca atgaacatca ctgttccagt tgctatccac ttagaccatg
     2401 gtgattacga agctgctttg gaatgtatcg aattaggcta tacttcagta atgttcgatg
     2461 gttcacatct accatttgaa gaaaacttga aattagctaa agacgttgtt gaaaaagcac
     2521 atgcaaaagg tatctcagtt gaatgtgaag ttggttcaat cggcggagaa gaagacggaa
     2581 tcatcggtaa cggcgaatta gctgatattg aagaatgcaa acaaatggtt gctacaggta
     2641 tcgactactt agcttgcggt atcggtaaca tccatggtca atatccagaa aactggaaag
     2701 gattagcttt tgaccactta caagcaattg ctgatgctgt aggtccagac atgcctttag
     2761 tattacatgg cggatcaggt attcctcaag atcaaatcga aaaagctatc tcaatgggta
     2821 tctcaaaagt aaatgttaac actgaattcc aattatcatt tgctgcagct actcgtaaat
     2881 atatcgaagc tggtaaagat aaagaaggta aaggattcga ccctcgtaaa ttattagctc
     2941 caggtaaagc agcaatcgtt gaagatgcta aatctcatat cgactggttc ggttcagcta
     3001 acaaagcgta agcatccaca taacagatac gatatatagg aatggagaac gacttttgaa
     3061 gtcgctctcc attctttttt acttaaaacg gcagaccatg ctcgtttctt ttttttatct
     3121 atgataaaat acaagcatat ccatgaaaaa agattaataa acaaggtgga aacagatgaa
     3181 aaaaatcgta atcaaaggaa accgaccttt agctggtgaa gtaacgatca gcggtgcaaa
     3241 aaatagtgca gtcgcattga ttccagctgc aatcttagct gattccccag tgatcttgga
     3301 aggtgtgccg gacatacaag atgttcattc attaattgaa attttagaga tcatgggcgc
     3361 aactgtccat ttttccaaca acatactaga aatcgatcct cgagaagtcg tatcgattcc
     3421 gatgccgcac ggaaaaatca atagtttgcg tgcttcttat tattttatgg gaacactgct
     3481 tggtaaattt ggcgaagcgg tagtaggcct gccaggtggc tgttatttag gccctagacc
     3541 gattgaccta cacgtcaaag gatttgaagc tctaggtgct gaagtgacaa atgaacatgg
     3601 tgccatgtat ttacggacag aaaataaagg attgcgtggc aatcggattt tcatggacgt
     3661 ggtttcagtc ggagcaacga tcaatgtgat gttagctgct gtaaaagcaa aaggacagac
     3721 agtaattgaa aacgctgcac gagaaccgga aatcatagat gtggcaactt tactgaataa
     3781 tatgggggca aaagttcgag gagcgggtac agatgtgatc cgtatcgaag gagtcgaaac
     3841 attacatggc tgtcggcatt tcatgattcc tgaccgcatc gaagcaggaa cctatctagc
     3901 attagcagct gcagtaggaa atggtatcaa agtgaaaaat gtgatctttg aacatttgga
     3961 aagctttata gcaaaattgc aggaaattgg tgtccatatg aaaatcagcg aagatgaaat
     4021 cgaagtttac ccctcgaaag aattaaaacc cgcaaacatc atgacctatc cttatccagg
     4081 atttgccaca gatctgcagc agccacttac tgcgttacta ttgatgacta caggcacttc
     4141 agaaatcatc gatacgatct atgcgaaacg tgtgaaccat gtgccggaac tagcacgaat
     4201 gggcgctgat attaccgtgg aaggaaatat gatcatcgtg aatggtccaa ataaacttca
     4261 cggcacagaa gtcgtggctt ctgatcttcg ggcaggtgct tgtttagtga ttgctggact
     4321 attggcagaa gggacaacga cgatttacaa tgtagattat atcttacgcg gttatgatca
     4381 tattatcgag aaactaacag ccttaggggc atcaattgaa atgatagagg aagtgggagc
     4441 tgaatgagcg attatttgac aatggcagaa ctagagaaca aaacgttaaa agaagtttat
     4501 agttatgcca aagaatttaa aattccttat tacagtaaga tgaataaaaa ggaattatct
     4561 ctagctgtga ttcgtgcaca agcagagaaa caaggatttt attatatgga aggaatattg
     4621 gatatcgttg gtcaagatgg ttacggtttt ttacggccaa tcaattatgg accaagtgca
     4681 gaagatatct atatttctgc ctcacaaatc agacgattca gtttgagaaa tggggacaaa
     4741 gtggcaggaa aagcccgtcc acctaaagaa tcggaacgtt attatggttt gatgcacgtg
     4801 gaaagcgtta atggaaaaga tccggaagaa gcaaaagaac gtccgcattt tccagcctta
     4861 acacctcttt atccgcaaag acagcttcaa ctggaaacaa caccgcaaag actatctacc
     4921 cgaatgatcg atatcttttc tccaatcggt tttggtcagc gtggattgat cgtcgcaccg
     4981 ccaaaagcag ggaaaacttc tgtattaaaa gaaatcgcaa atggtatcac cgaaaactat
     5041 ccggaagtag aattaatcgt tctactgatt gatgaacgac cggaagaagt aacggatcta
     5101 gaacgaagcg tgaaaggcga cgtggtatct tctacatttg atctgcagcc gcaaaaccat
     5161 actcgagttt ctgaattagt attggaacgc gcaatgcgtt tagtcgaaga taaacgagat
     5221 gtcgttatct tgatggatag tattactaga cttgctcggg cctataactt agtcgttcca
     5281 ccaagcggac gtacattgag tggcgggatt gacccagccg cattgtataa acctaagaaa
     5341 tttttcgggg cagcaagaaa catcgaagaa ggtggcagtt tgacgatttt agcgaccgct
     5401 ttagtcgata caggaagccg tatggatgac gtgatctatg aagaattcaa aggaactgga
     5461 aatctagaat tacaactttc acgtgaatta tctgaacgtc ggatcttccc agccatcgat
     5521 atcaaaaaat caggtacccg aaaagaagaa ttattgatgc caagtgaaga attagaagaa
     5581 atttggaaac ttcgaaaaaa tatgactgga gattcattag aatatacgga acaactgatc
     5641 cgctttttgc gtaaaacaaa aaacaatcag caatttttca aagaatttca agatgtctcc
     5701 tttggtaaaa aaaatccgac cagaaatctg aaaagataat tgcaaccttg actgaaacat
     5761 gttaagatgt taatgttgta aatcgacaaa tcaactctgg ttcagcaaat ggagcagggc
     5821 aaaggagcga aaaaatcatg aaagaaaaca tccatccaga ttaccaccca gtagtattca
     5881 tggattcaac aactggtttc aaattcttgt caggttcaac aaaaacttca caagaaacag
     5941 ttgaatggga agacggtaat acatacccag tcatccgtgt cgaagtaact tctgactcac
     6001 atccattcta cactggacgt caaaaattca cacaagcaga cggacgtgtg gaccgtttca
     6061 acaaaaaata cggtctcaaa gacgcaaacg ctgcggaata ataaaaacgc tgataactca
     6121 acgtttagca gactatctga tagggatagt ctgttttttt atgttttttg ccaatttaaa
     6181 acttcgtttg atgctgaaaa agtcgtagga tcataatgca caaaacaatg aaaaactagt
     6241 atatagtttt aattatccac ttgataaaaa caccttatat taacgaaatg aagaggggtt
     6301 atttcgaagt aaaaaaccat ttacctaaaa ttagaagcta agctttcctt tcaaggaagg
     6361 ctttttgttt tgttatagac ttgcgattgt ttgtgagcta gatcgttcat tttaatgact
     6421 tatacacgcg gatgatgggt ctttttcctc tgttcattca taatattgaa cggtacagct
     6481 agcgatgcta tactgacctc agttccaaga aaacgttaac attttttgtg ggggagaggt
     6541 tttttatgag gtttgatcaa ttgatcgcag aagaactgat tgttttcgat ccatcgatag
     6601 attcaaaagg atcattattt gagcgaatag gggatgtgtt gaaaaaaaca aataaggtaa
     6661 gaaatgttca aaaagtcgtt aaggcactat tcaaacgaga ggaggagatc tccacaggaa
     6721 tagaagatgg atttgggatt ccccatgcaa aaagcaaata tgtgacaaaa ccagcactgt
     6781 gtttttttca tacagccaaa ttaacggact atagcgggat agatgaaatg ccaattgaat
     6841 atatttttgc catcgtagtt cctgaaaaat cagcagatat gcatctagag attctcagca
     6901 cactctccag acgattgatg aatgcagatt ttagaaaggg aataaaagaa gccactacgc
     6961 cagcagatat catcagacag attagtcaag aatgaaaata gtagagtctt tgtccgagta
     7021 tcgatagtga agaaaaagga ggaaaataat catgaaaatc gttggtgtaa ctgcttgccc
     7081 gactggaatt gctcatactt atatgtcggc agaaaaatta gaagtaacag caagaaatct
     7141 aaaccatcaa gcaaaattcg aaacacaagg tgtaaaaaca gaaaatcagt tatctgaaca
     7201 agaaatcaaa gaggcagatg caatcattct tgcagtagat aaagaaatcg aattggaccg
     7261 atttgccggg aaaaaagtaa agcgtgtctc tacaagccga gcaatcaaag aaccgcaagt
     7321 agtaatagac gaagcattga gagatattgg cgtattcgtt gtgtctaatg aaaaagaacc
     7381 agctgcaaat gagaaaaagc cgacaatcta taatcatttc atgaatggtg tgaattacat
     7441 gcttccattt gtgatcgcag gcggaatcat catcgcactg agttttgcct ttgggattac
     7501 agcggctgat cccaactcag cagactataa tgtgttagca gctgcatttt caaggatcgg
     7561 aggagataca gcgtttgcca tgatggttcc tgcgttaggc gcggggattg ctacatcgat
     7621 tgctggaaaa gcaggttttg ccccaggaat tgttgccgga ttactggcct ctacaggtgg
     7681 ttcaggtttt cttggcggaa tgattggtgg cgtgctagca gggtatatct gtgattttct
     7741 ggcaaataaa attagcgtaa aaaaagaatt ttctgctatt tatcagctga ttgtcgtccc
     7801 ctttttatcg atcttaatta ttgggctttt gatggtattt gtgattgaac aaccgatcac
     7861 atgggtgttg gatgctttga caaattggct gaatagcttg ggaaatacat ccgggctatt
     7921 gttcggatta cttatcggga tcatgatggc agctgatatg ggtgggccga tcaataaatc
     7981 gacttcgact ttctcgatcg gtttgatgtc agctggagta aatgcaccga ttgctgcttg
     8041 tatggctgca ggaatgacac ctcccctagg tattgcttta gccactttat tattcaaaca
     8101 taaatttacc aaagaagaac ggacttcagg tcaatcatgc tggattttag gcgcttcgta
     8161 tattacagaa ggtgctatac cgtttgcggt atctgacccg atccgcgtga tacctagttt
     8221 gatgacaggt tcagcagtcg ctgcagggat ttccatggca gtaggagtta cttcaatggc
     8281 acctcatgga gggatttggg tgatgctaat tccaaatgtt atgaacaatt tacctatgta
     8341 tgtcttggca attgcatcag gtacagtcac tacagccttt atgcttggac tattgaaaaa
     8401 aacagtgaaa gagaaaaaac aagacaagtg gacgaaaaat gaaaatctaa caaatgaggt
     8461 ggaagcgtaa tgttatatac aatgaaagag ttattgaaag tggctaaaga gaatcaattt
     8521 gcagtaccag ccttcaatat ttgcagctat gatatgatga aagcaatcat cgaagaagca
     8581 gagagattag aagcaccagt aatcttagaa attcatcctg atgaaatcgc ctatttaagt
     8641 gatgagtttg ttgcggcagt aagagcctat gcttatacaa gtaaactgcc gatcgttatc
     8701 cacttggatc atggcggtac gatagaagat gtcttacggg cgattcgaaa cggctattct
     8761 tccgtgatga tcgatgcgtc tttgaaatca ttcgacgaca atgtacagat tacgaaaaaa
     8821 gtggttgcgt tagctcatgc ggtaaatgtc tcagtggagg ctgagcttgg aacgattggg
     8881 aataatggtt ctgctgaagg aggtgctgaa acaatcattt atacagatcc tgatcaagcg
     8941 aagatttttg tgaaagaaac aggaatcgat acacttgctg tagcaattgg aacagcgcat
     9001 ggcttgtatc cgaaaaacag aactccaaaa ttaaatatcg acttactgaa agaattgaac
     9061 caacaaatcg atcttccatt tgttttacat ggcggttcag gtaatccgga cgcagaagtc
     9121 agcgaagcag taaaatacgg agttgccaaa gtgaatttga gctcagatct taaaagcgtc
     9181 tttttcaatg ctttgagaga aatattgagt gaaaatccat cgatgtacga gccgaatgca
     9241 atttatccag cagctaataa aaaggtacaa gaagtggtca aacataaaat ggaaattttg
     9301 aacacagtag gcaaggctag tttatactaa aaataaaaat gtgtggtcaa gagggtatga
     9361 caatagtctt gtcataccct cttaaccaca cacgatgttc agaagtagtt ttttcgaaaa
     9421 atttcttctt atttctcgaa gccaatcact tctgtcacaa cctctttttt cctgttacta
     9481 tataaagtaa atggggtgat tttatgttga atgaagtgga gaatagacag caggagattg
     9541 tgaattatct aaaaaacaac caatttgcaa gagtcaccga tttgatcgag ttagtaaatt
     9601 atagcgaagc cacgattaaa cgtgaccttg tttttctgga aaaaagtgga ttgatccgta
     9661 gagttcgcgg tggcgccatg cttattgaca atcgaaaaat tgacgtgcct tacctgatga
     9721 agatcaccaa attagaagaa gaaacagaaa aacgtaagat tgcagataca gcttcttctt
     9781 tgattcgaga tgatatggtc ttattcttgg attccagtac aacctcttta cacttagtca
     9841 gaaacctagc aagatttgat ggtttgcaga tcattacgaa tggcatcgtc acagctgcta
     9901 tgttgagtga atatacaacg gcaaaagtat ctgtattggg aggggcaatt gttccgaaac
     9961 gagcaacaat caacggttca aaagcctata acgatgcatt gtcatattat gcggatttag
    10021 cgataatgag ttgtcgcggg ctagatttca gccaaggagt aacagaaact catgaaggag
    10081 aggcaatggt caaaaaagct tttagaaaac aatcagaccg gcttgccgtt ttagttacac
    10141 agcaaaaaat tgcacataaa ttcatgcatc aagccattgc ttttcatgaa atcgattatc
    10201 tggtgacaag taaagaactg acaaaagaac agtatgcgaa agtaaaggaa caccatatcg
    10261 aatgtctgta cggataaatt attacgtgaa tgttctaagt ggcttcctca tttttagata
    10321 ggaccttatc tttgctcgct tctctcagcc gatggtataa taatgaaaag gttatcttgt
    10381 tagataatct tactttttta tccaggagaa aggagtgcag aaaatgaaac atgtgaaggc
    10441 gttggtagtg aaggcaatca tgatctgggc gattctgtgg gtcgtgctga caggtttata
    10501 cggtgttagc tttatggatt caacgattgt tggggtaatt attgtagtca tgatctatgt
    10561 gctaggggat ctgttgatct tacgtaaagt agggaacatc gcagcgacga tcgcagatgc
    10621 tggttcagct gcagttatct tgtggctcta tctatatttt atgaatgata cagtcgatat
    10681 ttggatgaaa gtattgattc ctgctctatt gatcggtgtg gcagaatggt tcttccacaa
    10741 gtggttgcta agtcaaggca tcgtaccaga tgaacggaaa atggaataaa tcaacagtta
    10801 aactattctt atcgataaat gaaagcttta aaaataaata gatgtttaat taagaataat
    10861 cataaactat ttttactcaa aaaaccgctc agattatttt cagatctgaa cggttttttt
    10921 tatctattca tcaagatcaa atttcatttt tcaatgattc aatcaattcg ccctctgctt
    10981 catgactatg ttttcggcca ttaacaagat agagtgctgt acatatcaat ggaatgactc
    11041 cggcaatcac atacaatcct ctaaatgaag taagagattt tagcatcccc atcaaataag
    11101 gaccaacgcc taagcctagg tctaaaccaa taaagtaggt agataaagca atactgattc
    11161 gatgttcgcg gacgattttt aaacaaacag cctgtccatt tgacataaac gtaccataac
    11221 ctaggccgac taatgctcca gagataagca atgtcacact attcgtcgtt acactcagta
    11281 aaaataatcc tgctgttaag aataaatagc taggatacat cacgtattgt tctcctttga
    11341 catcgaaaat ccttccagtc aatggacgtg taaatgtgat gaccaaagca tatacgacga
    11401 agaaaaatga cccagcagca actaagtgga ttacttttgt ataagaagat aaaaatgata
    11461 aaacacttga ataagaaaga cccatcaaaa aggcaattcc tgtaatgaaa agtgctttct
    11521 tttcaataaa actattgata ttccatgagc gaagagatat tttttgttct tcagataatg
    11581 gaatattttt tacaggaaag acaaggcagg caatcgtaac aaagaagacc agtacaattg
    11641 aaaaccagat aatgaaacga aaatcagtca tattcagcaa aatcattcct aaaaatggcc
    11701 caacagcggc agctaaacta gtacttaatc cataataatt gatgccttcc ccttttttag
    11761 atgcagggat atatgcagta acgatagcat ttgtagctgt agaaactgta ccgtagccga
    11821 atccattcaa gaatcgaact aaatacaatg ccccgatagt tggcgtataa agatatgcta
    11881 atgttgaaag gagatagaag atcgcgccgc ctcttaaggt aaacttccga ccaaatagtt
    11941 ctaactgttt ccccatgaat aagcgtgcca gcaatgttcc gatgatatag atcccagaag
    12001 caaaacctgc ttggctcaag ctggcgtgta aggtgtcttg cgcaatgact gccatgatga
    12061 ccatcaacag ataataaatc aggtagacaa taaaattgat caatgtgatt ccaataaaat
    12121 atttattaaa taatttcttt tccatgtatg ttatacattc cccttaagct gaatatgctt
    12181 ttaatttttg tatttgaact tctacatcgt gataattgga tggatagcga tcaacaagtt
    12241 ggcgaataaa taaatctctt gtagacataa acagatttaa aaattcttcg cattcgtttg
    12301 ttggattctc atccactgct ttgatcaatt cgatcgagtt ttgatacgca tcatttaaaa
    12361 agactaaaga agctttcata tcaaatcttg tttgatatgc tgtttctaat cgattaatga
    12421 ttgtgttcat ccagtaattc atgttttttc cctctattct taaaaaattt tacttctgca
    12481 gtataacgaa taaaaaaaag gaaaaaaatc gcataagtga tggaaaaagg tacttaacaa
    12541 atgttaatat cattagtaaa atacctttgg actttaaaaa gttttaaagt actgtatgat
    12601 ctttttagat gaaagtttgg atgggggagc atcattggat agtagagtct taaaggaata
    12661 tttggataaa aatgattttc cgattatacg caaaggatat cataaatatt tgacattcga
    12721 agggatggaa gattcccata cgtatatctt gaaagaggga acggtaaaag caagtgttat
    12781 ttcaaatgat gggagagaat ttaaccttcg ctatatcaaa gatcttgaaa tcgtctctct
    12841 tttaaaagac gaatattcac aattcatcga ttcgccgtat aatattcgag ttgaatcaga
    12901 atatgcagag ttttatcgaa tcaatcgtgt gcgcttttgg caaaatatca accaagaccc
    12961 aaagctacaa cactatgtaa aagagtatta ccgctatcgt ctgctttatt cgatgaaaaa
    13021 aatgcagcaa atgctattaa acggaaagct gggagctgtt tgtacgcaac tttacgaatt
    13081 atatgattta ttcggtgtgc ggacagaaga agggatgctg atcgattttt cgatcacgaa
    13141 tgaagaaatc gccaaatttt gtggcatctc aactgctagc agtgtaaaca gaattttaaa
    13201 acaactgaaa aaagatggta ttatcaccgt taaagaccat catatcgtta ttttgaatat
    13261 ggacttgtta gaagacaata ttatcttttg atgaacaaag aggttgtgaa atcagcgttt
    13321 tgacctgagt attagagaaa caaacggaaa aatagttcct catattttgc cgttttttga
    13381 gctaataccg agggtcagct gattgaacac cgtttaccaa gaggttgtga gcctgccgtt
    13441 taactctgag tcacaaggaa caatttcaaa aaacagcttt tcatgtttga tgaatattgt
    13501 gacttgtgac cgaggagttg gtaggagaac accgtttacc aagaggttgt gaaaaagctg
    13561 ttctgcatca agtaataaga agagccaagt aaaaacagtc tcttatgttt ttaacttggc
    13621 tggacttatc accgtagatg taagcttttg aacgccgttt atttgcaaca aaaggggctg
    13681 tgacacgatt tttgtcacag cccttttttt gcgaacattc atttagcaat ttttagaaac
    13741 ttctgccgcc accgccaaaa gtcccgccac cgctagaatg ggtagtactt ccgccaccgc
    13801 cgcttccagg aggtgggctt ttggggattc ttcttgtcgt gacaaaagaa tttgtcagac
    13861 gatcggtttt ttcagttaat ttgatactgg atttttctcg gaatggatat ttgtagatgc
    13921 ctgatttcaa ttgataacgc gaaacagtca caaaataaaa gacaagacta aggataagag
    13981 ccaaaacaac agcaatgatg atttctactg tcgtcaaaac cttatagcgt gtaattttgc
    14041 cagtttcttc gtctacacga taatgtcctc ctggaactcc gtcttccaca tatgttgctg
    14101 atttagacag gtaagctttt gcagcattgt aataggcagc atttgtcatc tgatcataaa
    14161 catcatctaa gatcgaatcg attctgctat cagtcaaata atcgatcata ttaccagaag
    14221 tcgaaatgta gatttcacgc tgtcccatat ctaatagcaa tactgagcca ttttgatcat
    14281 ttccgactgc gtctcttaag taattatcag caaagtctct tggttcttct gaattagatg
    14341 tagtcgttac gatcaagact cgtcctttga ttttttcatt cagcttttct gcctgttgtt
    14401 ccaaagattg gatttgatct gttgtgaata acccagcctc atcatttacc gtagctgtat
    14461 ctgcatgagt agccaccggc cagccaacac ttactagcaa aagaaagaat aaaaaaaact
    14521 ttttcatcat atcaagtaac ctccgatcag aaagaggatc gctagcccag cgaaaatccc
    14581 aagagcggtc agtcccaatt ttttctggct gatcggcagg acaccgctga cctttcctgt
    14641 ttgtccattc attgcgtagt agtagacttt tttacttgag tcattgcttc gataagtaac
    14701 aagccatacc ggcagtaata cgtaattatt tacttcgttt gtgattgaag cagaagttcg
    14761 cacaccggtc aatgttgtat atccatttgc ggtatctttt aacagcttct ctgaatagtc
    14821 ttttaattca gattgaatct gtgatttgat tgcctcatat tcgatatctc ttttttctgc
    14881 ttggaacccg gctaaatact ggcttttaaa atcaattgct ttatctaacg ggaaaggttg
    14941 cacgccttcg accatctttt gttgcgtatt ttttgataag gcatttttta ctagttcttt
    15001 aaaagaaagc ttgcctgctc ttgaaacagc aaattgcttc gtttctgtgt attcgatgtc
    15061 acctatccgc catatgcgga taactgtacc attcgcctgc atggcaccat ctacttcagc
    15121 atctgtagcc caataaggga aatacacacc tgtcaactta tcgatttgat ccttattgaa
    15181 aaaagctttg ggaacaaacc attttttctt ggtccaagcg agaaatctgt tgattgcttc
    15241 ttctttctcg acagcaaaag gaagtacttt atttggcaag aattctccgc ttaaacgtcc
    15301 cgatagtacg acgggattat ggcagtagta acaataagtc gcagcagttg tcgcatcagt
    15361 cacgatctct gcaccacagt tgggacaaag aaaaagatct atcgctgcgg tattatctgc
    15421 agattcgtcc gctgtatctg aaaaaccgcc tgcctcgtca accgctttgc gctcggcttc
    15481 attcatatcg gcaagctgtt cttgggctgt aaaagtaaaa tcttcggtag gtgaaggttc
    15541 ttctttgctt tcttcgccag ccatacgcgc ttctttttgc ttttgctcat attggctgac
    15601 ttcttcttct gtatagatat tcaggcagta ttcgcaatga aatttttggt ctttgggatc
    15661 aaaagtcaac ggaccgccgc aattaggaca tttatgtgta agaacatcca tcagctgcac
    15721 acctttctaa ataaattaat taacggggat tcctttaaac ggtttaccac agtttggaca
    15781 gaatttaggg atgccgttgg aaagatccac gatctcattg cattcactgc atcgcatttg
    15841 tagcttaggc tgaacgtttt ctacaggttt ggcagctccg cagttggaac agaattttcc
    15901 ggaattttct gtcccgcaaa ctggacatgt ccaagtatcc gcatttcctt gtgcagtttg
    15961 ttgattttgt ttttctgctt gttgtttgat ttgttcttgg ttattttgag ctgcttgaga
    16021 aagataagaa ccatttgcat tcattcccat acctacaccc ataaagccgg tcattgctcc
    16081 tgcttcattt tttccggctg cttccatacc acgtgcaatg gaaccttgca cgtatccttc
    16141 acgtacgcta ggatcaccta gcatcgcccc ttcgttacgc atattgatca gtttcacaga
    16201 atcatcggtg taagaaatgc tggctacagc cacagaaaca atctccatcc cacgtaattc
    16261 acgccaagaa tcatccaatg cagtatccat gtatttgctt aattccaaac tcttagatgg
    16321 tacataagaa atccgttgac catctgcaga catctgattg attgctgatt gtagcgctgt
    16381 taggaattca gctaaatact gctcattgat atcattgatt tccacttgtg ttttgttttt
    16441 agggattgcg tttgtataaa ataaaatcgg gtcaacgaca tgaatagaat aagtgccatg
    16501 tgcccgtaag aataattctg cgttatagaa attgtcaaag tagttcaacg gcgcgcttgt
    16561 accgaatttg attcccttta tttcttggag attgatataa aaaacttgtt gtttctgcgg
    16621 tgtgacgccg ccaaatttga atcggtcaaa agtttcagca attgcttctt tcagtgttcc
    16681 attgaacatg gaaggagctg cgtcattttt aatggtgtaa taaccttcct cagcggtata
    16741 atcgatgatt tttccaccat cgaccaaaag catcatcatg tttggataga catggacgac
    16801 tgttccgtct gttaaaacat cttctgttcc tttacgattg gagccacgtt tgtcatcttt
    16861 gcggactttg acaccttttg tcatgaccgt tgtatcactc atattgtctg gttcgatgac
    16921 ttctagccat tgatctgcca gaccgccgcc aaccatactt gttgctgctt tgataagacc
    16981 cataaaaaat tcctcctctg attcagctag tgccaaacaa ttattgttta tttattatac
    17041 cataggaaga tgtgaaactt ctcggactag agacggaatt gaaatggaaa attgcgtatt
    17101 gtttaaatac ctgaaaaagc cgagaaggat agtagttttt ttacctaata tcacgtataa
    17161 taagagatag tgaatttttt aaaaaagggg gatccttatg gcatatcaag cactttatcg
    17221 agtctggcgt tcgcagcgtt tcgaagacat cgttggacaa aaagctgtga cacagacact
    17281 aaaaaatgct attgtttccc acaaaacctc acatgcctac ttgtttacag gacccagagg
    17341 aaccggaaag acgagtgcag caaagatttt cgcaaaagca atcaactgtc cgaacagtaa
    17401 agacggtgag ccatgcaacg aatgcgaaat gtgtcgatca attactgctg gtacgcaaga
    17461 agatgtgatc gaaatcgatg ctgccagcaa taacggagtc gaagaaatcc gatttatccg
    17521 tgatcgggca aattatgcgc ctacaaaggc agcgtacaaa gtgtacatta ttgatgaggt
    17581 acatatgctt tcaacaggag ctttcaatgc cttgttgaaa acattggaag agccaaaaga
    17641 aagtgtcata ttcatcttag caacgaccga acctcacaaa ataccagcta cgatcatttc
    17701 acggactcag cgatttgatt ttaaacggat caatacggca gatattgtcg aacatcttga
    17761 atttatcttg aaagaatcga ctatcagcta tgaaccagct gcattgaatg tgattgcccg
    17821 ttctgcagaa ggcgggatgc gggatgcatt gagtattctt gatcaggcaa tctcgtttag
    17881 tgaaggaaca attactttag atgatgccat gcaagtgaca ggtagcttaa ctgacgaaat
    17941 gatggacagt tacttgagtg cttgcgtgaa aaaagaagta ccagaagcat tggaaacatt
    18001 gaaccaaatt ctcgcagccg gcaaagaatc acgtcgcttt ctagaggata tgttacaata
    18061 ctgccgtgac ttgctgatgt atcagcaagc acctaaccta gttgaggaac aatcgagtac
    18121 gatcagaagt acatttacag aattggcaca aactgtttca gcggatcagc tgttttcaat
    18181 gataaagatt ttgagcgata ctcaaaatga aattcgtttt accaatagtg ccgctattta
    18241 tttggaagtc gctacagtca aacttgcaca gctaccgtct gcttctgttc cttcagcaga
    18301 tgtagcattt gcagatgatc agatagtaaa acagctacaa caagagatta aggaactaag
    18361 aaacgaaatc aaggaactac gcagtaatac aggaattgag aatacaaaag tacagccggt
    18421 acagcaaaca aatcataaaa aacagacaag tacctatcgt gtaccgacgg aacgagtata
    18481 taaagttctg cgggaagcga caagacagca tctgaaccaa gtaaaagaag tatgggaaga
    18541 cttgctgatg agtctatctg tcactcagcg agcaatgctt aaagcaagcg aaccagttgc
    18601 agctggacct acaggactag tgattgcgtt tgattatgag attgtttgtc aaagagcagc
    18661 aaatgacgaa gaattgcagc tttcggtaca caacaactta agccgaatga tcaaagatta
    18721 tgcgcctgat cctgtttata ttacaagaga aagctggcca gaacttcgaa gaaattattt
    18781 gacgcaggca caagaagagg gaacagaaaa ctcttttgct tctgaagact cagaaggaga
    18841 aatcgagttg cttcctcaag aacaagaagt accagaagaa acacttgtag taaaagcaga
    18901 ggaactgttt ggctccgaag ctgttacagt ggtagatgat taataaaaag gagtagataa
    18961 ttatgatgcg tggaatgggc aatatgcaag gcatgatgaa acaagtacaa aaaatgcaaa
    19021 aagaaatggc agaagcacaa gagctgctaa ataaaacaga gtttacaggt aaagccacca
    19081 atgaattggt caaagcggtt tttacaggcg atcgtcgcat gaaagatatc caaatcaatg
    19141 aagaagtcgt tgatcctgat gatatcgaga tgcttcagga tcttgtcgta atggcagtca
    19201 atgatgcgat cgcacaaatt gatgtggaaa ctgagaaaaa attagggaaa tacgcaaaag
    19261 gtttgccagg attttagttt taaactgaag gagtaatgaa tatgcaatat ccagaaccaa
    19321 ttgcgaaact gatcgacagc tacatgaaac ttccaggaat cggtcagaaa acagcgacaa
    19381 gactcgcctt ttatacgatc gatatgaaag atgaagtagt aaatgaattt gctaaatctt
    19441 tgttaagtgt aaaacgcgat ttgcattttt gtagtatctg cggaaacatc acagaagaag
    19501 atccctgcga aatatgtaga aatccttcac gtgatcgaag cgttatccta gtggtcgaag
    19561 agccgaaaga tgtaatggca atggaaaaaa tgcgagaata tcacggactt tatcatgttc
    19621 tccatggtgt gttatcaccg atggaaggga caggtccaga agacatcaat atttcttctc
    19681 tgttgcagcg gctgcatgat gcagatgtaa aagaagtgat tatcgcaaca aatgcaacaa
    19741 cagagggaga agcaacagct atgtatctct cacgtctgat caaacccgca ggtatcaaag
    19801 tgactcgtct agcacatggt ctgtctgtag gctctgatat cgagtatgca gatgaagtta
    19861 ctttattgaa agctgtcgaa ggaagacgtg agttataaat ttctaattaa gtgttttttc
    19921 agatacaata taaaagcagc acaagagatg ggggcaacaa tgtgaacgga ctatttatta
    19981 caatcgaggg gcctgatggt gcaggaaaaa ccagcatctt gaatgaactt taccctcttt
    20041 tgaaagaagt agcaaaaaca gaaatcattc aaacaagaga accaggaggc attccgattg
    20101 ctgaagagat ccgagcagtg atccttgatc caaaaaatga tcggatggat gaacgtacgg
    20161 aagcactgct ttatgcagca gcaagaagac aacatctagt cgaaaaggtt ctgcctgctc
    20221 tggcaaaagg gaaaatcgtt ttatgcgata gatttgtcga cagttctctt gcttatcaag
    20281 gtgcaggccg acgcatcggc gttaccgaga ttgctcggtt gaatgagttt gctacagaag
    20341 gtactacgcc ggattttacc ctttatcttg acgtagattc agacaccgga ttaagaagaa
    20401 tcaagaaaaa cagacaaaat caaatcgacc gtttagattc ggaaggtttg gaattccacc
    20461 agcgtgtacg tcatgcttac ttaaagctgg cagaagagaa tccggaaaga atccacaaag
    20521 ttgatgcaag aaaaagcttt gaagaagtgc tgcaaacaag ttatcataca attattgaac
    20581 agtatccgca attttttgaa aactaggaag gtgaacgttt atgaaaatta ttttagcgat
    20641 tatccaagac aaagacagca accgtttatc aaatgaattg attgatgcaa atatccgtgc
    20701 cacaaaacta tcatcgacag gcggcttcct taaggctgga aacagtacgt ttatcgtggg
    20761 tattgaagat gaccgtgtag aagaagcatt ggaaatcatc aaaaaaacgt gtgaatcaag
    20821 aaagcaattt gtatctacac ctgttacact ggatatttcg atggatgggg gcgtccctta
    20881 tccagtagaa gtagaagtag gcggtgccac agtcttcgtc ttacctgttg aaggattcca
    20941 tcaattttag gaggagctga gctatgaatc aagaaataaa cttggctcaa atgcaaccga
    21001 tcgtgtatca gcagcttcaa cggagttttg agcatgggcg tatcgctcat gcttatcttt
    21061 ttgaagggga gaaaggaaca ggaaaacatg aagtaggtat atggcttgca cagcacctgt
    21121 tctgtaccca aatgaaagac aatctgcctt gtggaaaatg caacaattgt cagcggatcg
    21181 aaaagaaaga acatccagat gttttggtta ttgaaccgga aggacaaacc atcaaagtcg
    21241 atcagatcag acgtttgcag acggaattca gtcgcagcgg ttacgaatca agaaaaaaag
    21301 tttttttgat caaagaagct gaaaaaatga atagcagtgc ggcaaacagt ttgttgaaat
    21361 tcttggaaga gccccctgga gattttttag cggtgttaga aacagatgca gtaggaagaa
    21421 ttctccctac tattcaatct cgttgtcaag ttctgcattt tcaagaactt tcgaaagaag
    21481 cactgatcca tcgattgcaa gaagagcaga ttccattgga aaaagcaaaa cttttggctt
    21541 tcttaacaaa cagtttagga aaagcagttg aaatctctca agatgaatgg tttaatgatg
    21601 ctaaggactt gatttatcaa tggttcgttt acttgcaaaa gcaagatact caagccttta
    21661 tctatgtcca aaaaaaattg gtaaaatcat ttaaagaaaa aacacaacag tttacaggat
    21721 tatctgtctt gatgttttat tatcaggaag cacagcgtca agcagtagca aacggcgaat
    21781 atggcaaagt gaaacagata aaccagataa ttgaacggat attgatcgca gagcagaaac
    21841 tacgcagcaa tgtcagtttt caggctgttg cggaacagtt cgttttgcat acgatttttt
    21901 agcttgccgc aaaggatgat gatcaaggag gatggaaatg gtagaagtag taggtgtccg
    21961 atatagagat gcaggtcata tctattattt tgcaccagga gactctgaat atgtttatag
    22021 tgacaaggta ttagtcgaat ctcagcaatc cttacatatt gctactgttg tccttcctaa
    22081 aaaagagatt gatcccaatg accttcctga agctatccgt ccgatcttgc ataaagcgtc
    22141 acaagctgaa ttagaaaaag acaaaaaaaa caaaaaagat gcttatgatg cattgaaaat
    22201 cgcaaaagaa aaagtgcagt tgcgtgagtt ggaaatgaaa atggtcaaag ccgaatacac
    22261 atttgatcga aataaattga ttttttcttt cacagcggac gggcgaattg actttagaga
    22321 attggtaaaa gatctggccg ctgtatttcg tactcgtatt gagcttcgac aaattggtgt
    22381 ccgcgatgaa gcaaagataa taggcggaat tggtccatgt gggcgtccat tatgctgttc
    22441 ttctttttta ggcgatttcg tgcctgtatc aatcaaaatg gcaaaagatc aaagtttatc
    22501 tttgaatcca acaaaaattt ccggtttatg cggacgcttg atgtgctgtc tgcaatatga
    22561 gagcgaagaa tacgagacag cgaaaaaaga actccctgac tttggaaaag taattgctac
    22621 acctgatggt aaagggaaag tcgtagggtt gaatctgctt agccgtatcg ttagtattcg
    22681 gctgactgga cgggaaaatc ctgtcgaata tgaatgggat gaattgaaag aatttatcca
    22741 agtaggtgag atgaatggat aagagatcat tatacgacgg attgaatcaa ttagaatcag
    22801 agttgagaaa tacattaaca gaacttgttg aaatgaagca tgctttgcat gagatcgtag
    22861 aaaaaaatac aactttggag atggaaaatc aacgtctgcg ggaacacctg cgggaattga
    22921 accaagttgc acaagctcca gctgaaaatg tgaagcagga actttctaag tctcgtatga
    22981 atcttgaaaa aatttatgaa gaaggattcc atgtctgcta cgatttatat ggatcaagaa
    23041 gggacaatga cgagccttgt gcgttttgtc tagaagtgat ctatcgcgaa agcggtcgat
    23101 gaccaataac aagaggagac tggagcggag aaatactgct ccagtctttt tattaaggag
    23161 attgtgacag atgtataatc aaaaaagttt caagggaaat ccggtatatg gggatttata
    23221 tcttgtaccg acgccaattg gaaatttaga agatatgagc tacagaagtg ttcgtatgct
    23281 gcaagaatcc gatcttatcg caagtgagga tacgcgtaat acacaaaagt tattgaatca
    23341 ttttgaaatc catacacccc aaaaaagttt acatgaacat aattataaag aacgtgtccc
    23401 tcaattgatt gctttgttaa aagaaggaaa aacgattgct caagtcagtg acgcaggaat
    23461 gccttccatc agcgatcctg gacatgagct agttttagcc tgcataaaag aagggatttc
    23521 tgtgatctct atcccaggac ctacagctgg gatgacggct ttgattgctt cagggctagt
    23581 gcctcaacca tttttatttt atggcttcct tccacgtaag aaaaaagaac agaaagaaac
    23641 attgaaaaag ctaaaagaac agacagttac gctgattttt tatgagtcac cttatcggat
    23701 cactgctaca ttgacgaatt ttttagaaat ttttggaaat cgccaagtcg ttttgtgtcg
    23761 ggaattgaca aaggtccatg aagaatacct tagaggaagt acggaagagc tactcgctta
    23821 tatcgaagaa catccagtaa agggtgaatg ttgtctgctt gtagaaggga atttgttcga
    23881 aatgccagtg acggaagttg atgaagagtt gggaacgtta aaggaacaag tagaagcaaa
    23941 gatcgcttca ggcgaaaaac caaatgctgc tatcaaagca gtagcgctga aaaatgggct
    24001 gaaaaaacaa gaagtctacc gtcagtacca tgaattagac tgagatagta taagttgtct
    24061 ataatgaaag actaatgagg aaatcagcta ctaacttttc aaatctcttg aacgcgaacg
    24121 aatgttcttg tataatgaat aagaggtgag gatgatggag ctgcaatttc ctttacataa
    24181 tgatacatca agaaaaatca ttcatattga tatggatgct tttttcgctt cggttgaaga
    24241 acgagatcat cctgagtttc gcggacatcc attagtaatt gccagacacc ccagtgacac
    24301 tggtgggcgt ggagtagtga ctacagcaaa ttatgaagca agaaaattcg gaattcattc
    24361 agcaatgagt gcacaaaaag cgtatgagct ttgtccgcaa gccatcttta agcctggcga
    24421 ccatcataaa tacagcgata tttccaaaca agtacgggaa gtattctatc gttatacaga
    24481 tattgtggaa ccagttagta tcgatgaagc gtatcttgat gtgaccgaaa ataaaatcaa
    24541 ttgcaagtca gccatcaaaa tcgcccgaat gatccaagca gatatttggg agagtgtcca
    24601 gctaacatgt tctgcgggag taagttacaa taagtttttg gccaaattag catcagacta
    24661 tcaaaaacct agaggaatca cggtagttgc ccctcaagac gctgtgccat ttttaaaagc
    24721 tttgccaatt gaaaaatttc atggggtagg gaagaaaact gtacctagaa tgcatgagct
    24781 agggatttat acaggaaaag acctatatga atgcacagaa atgatgttga ttcgaaattt
    24841 tggaaaaatg ggctattctc tttatcgtaa agtcagagga atccatgatt ctccagtaaa
    24901 cgttacaaga gagcgaaaat ctgtgggaaa agagcatact tatgggcagc cgttgcaaac
    24961 ggaagaagca gtgctgactc agttgcgcca actagctgaa aaagtagaag aagcactgag
    25021 aagagtccaa aaacatggga aaacagtagt tttgaaggtc aggtacacgg actattctac
    25081 tgtgaccaaa agagtgacat tgccagaata tatctataaa aaagaagcac tcttttatca
    25141 agcaagcctt atatgggagg aaatcttggg agtagaaaaa ggaatccgat tgttagggat
    25201 tactttgacg aatctggacc cgatgactta tgaaaacatc gtccttccat tatgggaaaa
    25261 tcaagaaata taaaaacagc tttgagagtt gaaaccggaa atccgttttc tctcccaaag
    25321 ctgtttttat atcaagtctt gctttttgat ttgttcatag gtttctgttg catccgtaca
    25381 tttttcccaa atcacaactt gattggattc taaagatttt tggacgtcaa tgatccgctg
    25441 gttgctactt ccgcgaaatt gtagttttag gtcacgtttg cttaattcaa agcgtccgtc
    25501 aacaaggata tcgatttgcg aaaggagttc taatttatct tctgaatctt gtagcaattc
    25561 atcaaaggca tagcctgtcc atgaccagat atcttttgtc gtaccgaact cttttcggat
    25621 acgtcgtacg acttgcagac aaacatctgt attcaaaaaa ggttcgccac caagtaaagt
    25681 taatccttgc acgtaatcgt tgcgcaagtc agcgatgatt tgttcttcta attcaggtgt
    25741 gaatggtttg ccatatcgga aattttgtgc agctttgttg aagcagcctt cgcaggcaaa
    25801 taagcagcca ctaacgtaaa gactgttgcg gacaccttca ccatcgacaa aattaaacgg
    25861 tttgtagtca gcaatatagt tttggctgta gtcttttgct aaccattctt ttggttttgg
    25921 atttctcata aaaaaacttc tttcatttaa ggttcaaaga tatagctatt gaagataaaa
    25981 tctactcgac cacataaata agaacaaact gacctaaatc cattgttcaa gaaagcttgt
    26041 tcttattttg atagagggcg ttcaaagata caaagccatc gaaagagggt ggtgaaaaaa
    26101 gacttgtcat agcctcttat tctgaataaa cggtgttcaa aagcttgcat ctgcagtaat
    26161 aagcccaact aaacgaaaaa tatgaggaac tatttttgtt tagctattct tattacttga
    26221 tgcaggacag ctttttcaca acctcatttc atgtgtttca cacgtgagga aatttcttta
    26281 tggcggccat gtaccatcgg acgagcttgc ggatttccta aatagccaca agtacgtttg
    26341 actacatcac atgtttttgg atcatgattt ccacattctg gacattcgaa ccctcgttct
    26401 gtcggtttga aatcaccttc gaaaccacat tgatagcaat gatcgattgg cgtattcgta
    26461 ccaagatacc cgattttgtc atatgcaaaa tcccatacag cttctaaagc tttcggattt
    26521 tgttggagca tagggtattc acagtagtgg atgaatccac ctgaacaata ttttggataa
    26581 tccttctcaa attccaattt ttcaaatggc gttgggtttt tacgcacatc atagtggaaa
    26641 ctgtttgtat aatattcttt atctgtgata ttttcgatga tgccgaattt ttctgtatcc
    26701 aaacggcaga aacggtcagt tagactttca cttggagttg aatagacact gaaatgataa
    26761 ccatattcat ccccccatgc atctgcatta gcttttaatt ctttgacgat atcaagtgtg
    26821 aaagcttttg cttctgggtt tgtttcccat tcgccaccga agaaagctga agcaacttca
    26881 tacaatccaa tgtagcctaa cgatacagta gctcgacggt ttttgaataa ttcattgaca
    26941 gaatcggtac gtgaaagacg tttcccgaaa gaaccataca tatataaaat tggcgcattt
    27001 gctggaatgg cttctttaca acgttctaca cgatagagaa gcgcatcttt catgatttct
    27061 aagcggtcag acagtaactg ccagaattta ttctgatctc cttttgattc gatcgcgatt
    27121 cttggcaaat tcaatgtaac aacacctaaa ttcattcgtc cgacatttac ttcttgacca
    27181 ttttcgtctt tccacccttg caagaatgaa cgacagccca taggtacttt gaaacttcca
    27241 gttaattcaa cgattttatc gtaattcaga atatcaggat acattctttt cgttgcacat
    27301 tccaatgcta gttgtttgat gtcgtaatta ggatcttcag ggtttaagtt cacacctttt
    27361 ttcaaagtga agatcagttt agggaagata gctgtccgct tttcactccc taatccattg
    27421 attcgaatct gtaaaattgc tttttgaatt tctctttcaa accagttttc tcctagtcca
    27481 aatcctaatg aagtgaaagg agtttgtcca tttgaagtaa ataaggtatt gatttcatat
    27541 tccaaacttt gcatagcatc gaagatatct tttttggttt ttgcttttgc atattctttt
    27601 tggcgttctg gcgaatcgat ccattcttcc gcgtctttta gatgtttttt atagttcaat
    27661 tctgcaaaag gagcaagcaa ttcatctgtt cggtcggcag aacagccacc atattgactt
    27721 gacgctacat tggcaataat ctgtgagatt tgtgctgtgg cggtctggat agattttggc
    27781 gattcgactt cggcattgcc gattttgaac ccattgttca gcatgccttt gaaatcaatc
    27841 aagcagcaat tcgtcatcgg tgtataagga tgataatcca aatcatggta atggatatcg
    27901 cctttttgat gcgcatttgc tatatgtggt ggcaacattt tcagtccgat tgattttcca
    27961 acgatcccgg ctgtcagatc tcgctgtgta ttgaaaacat tactgtcttt atttgcgttt
    28021 tcgttcacga cagtttggtc cttgttgatt agcttttcaa tcgaaaagtt gatatctgtt
    28081 gctttactgc gagcaaaatc tcgtttggta cgataattga tgtactcttc tgctaattca
    28141 tattcgtttt tttctagtaa tatatgttcc acgatgtttt gaatctcata gattttgaca
    28201 ttttcagcaa accggctttt gatttcttgg acaaccaagt ctgcaatcat ttgaattttt
    28261 tcataagtaa gtggtgataa tgagccgtga attttctttt ctgctttgat cagtgcttca
    28321 ttgatctttt gttcatcgaa atcaacatgg cggccatcgc gtttgatgac tttcatctgg
    28381 gatgtctctg gctgctgagt aacgacagtt ttttcttcat aatttacttt cattatcttc
    28441 aactcctgct aaattgttct tgtctatcat aaaacaaaat cggggaagga gcaactaaat
    28501 atcggctata tatagtattc aatgtgctgt ataattggat ttctatacta tatgtagtgt
    28561 atattttatt gtgaaaaaag gtgaaaactt gtagctggtg gatttgtttt ttgcatattt
    28621 aattttgctg aataaaataa ttgaataaaa ttttttattc agtccaagta aaagtgacaa
    28681 aaacataaga gcaaattatt cgtagatatg ctactatgca aataaataga aatgaaaaat
    28741 gaaagaggag gagaagaatt ggaaaagatg atcgaagtga aacatctaag taaaagctac
    28801 ggcagcaaac tttctcccgt caaagcctta gataatatct cattcacagt agaagaaggc
    28861 gagtttgttg ggattatggg cccaagtggt gcaggaaaga caacgctgat gaatatcttg
    28921 tccacgatca atttgcctac gatgggcagt gtctcgatcc aaggaaaaga aatcacaaaa
    28981 atgaaaaaca gtgagctcag tgactttcgt cgaaaaaaac taggattcat ttttcaagag
    29041 ttcaatttga tcgataccct aaatgcaaaa gacaatatcc tattgcccct agcagttgaa
    29101 cggatgacta aagaggaaat ggaaaagaga gtaagacatg tagctcaatt gttgaatata
    29161 gaagaactgc tcaaacggta tcccgatgaa ctttctgtcg gacagagaca acgaattgca
    29221 gctgctagag cacttgttgt tcagccacaa gtgatttttg cagatgaacc aactggctct
    29281 ttggactcga agtcggctac agaattgttg aactacttga agaccatgaa tcaaaaagaa
    29341 aaagcaacga tactgttagt cacccatgat ccatatacag caagctactg cgatagaatt
    29401 ttatttatta aagatggtgt gattttttct gaagttgtca gaagagggac aagaagagag
    29461 ttctttgaga aagtaataga catgcaggcg acgattggcg gaggaggaaa aatgaatgct
    29521 gtataaacta gccgttaaaa gtttttttcg gcagtcacga ggctatcttg tttatttttt
    29581 cagtttgact ttatccgtaa tgatctatta ttccttcagc gcaatgacct acgatcaatc
    29641 tttagtcaga agagcaagtc aagatacttc gatagatggt ggattgagtt ttggtggatt
    29701 tatcattact gttgttttgc tttttttcgt attcagtgcc aaccgatttt tcttgaatag
    29761 aaggcaaaag gaaataggca tctatcagct ttttggtatg aataaactgc agatatcagg
    29821 tatatatgtg ttagaaatca tgataattgg attgttcgcc tgtatttctg ggatattact
    29881 cggtattatt ttttccaaac tcttttcaat gattttggtt cgaatgatgg atatggatct
    29941 tactagtcca ttttttatct ccattccatc agtagtagat actttatttg cttttttcat
    30001 catcctttta gcagtatctg tatattcaat atggaaaata tggcgctatc caatgattcg
    30061 aagttttgga gaaaaagaac aagcagatag ttcaacaatg cgcttcagga caaggcaccg
    30121 attactcgca gtcatcgggt tactactttt aacgagcgga tattttggag ctagtcattt
    30181 tagggaaatc acttcatgga tgatcagtta tcattatctt gatacattag ttttcttttt
    30241 tcctttttta atttttttcg tttgtgtggt cggtacttat ttgtttttct gttatacatt
    30301 tcggttattt ttgatcatat tgagtaaatg gaagatttat tactatggta tcaatttttt
    30361 gataatgggt aacacgcaag ttcatttgct caaaagttgg cgtacaaatt ctctgattac
    30421 agtcattctc ggaatttctt taatgacaat tggcggcacg gctagtatct ctactatttt
    30481 atcctaccag gagaaagttt cctccccgat ggactatcag tttgatgttg agacagagaa
    30541 aaaaatcaag ccgattctag cagaggaagg gcagaagata actaaagaaa tcaccttgca
    30601 ttataaagtc attgggagta tctatagtca aaatctcgaa gaacctatgt ttcaaactgc
    30661 taatctgatc acggaaacac aatatcaagc cttcagaaaa gttaatccag atttgccgaa
    30721 catttctttg aaatcacctg gtcatactgt tttgcttgat caaatgcaaa ctctttttcg
    30781 gaatatttca ttatatggat ctagtattgg actgccagat aatcaagaat tgaagattca
    30841 acaaatgctt ccaagtgtat taggtgacga aacaatggta tatctaagtc ctgtactgat
    30901 tgtagatgat accgtttttg aaaaagcaaa aggtgtggat tatcaagtag tcaatgtaga
    30961 tgtatcaggt ggaaataatg aaaaaataga tgaacgactg agcaatgacc tgtcgataaa
    31021 gtggctcaat gctgtttatt attcttatga tatcgtaaat ggaagtctac aaggagaaat
    31081 tagcacaaag aaacttccgg atgaccaaat gaaggaagca caatttagcc aagaaagctc
    31141 tcgtttgaat ttctcttcca gctattcgaa attgcgtgca gttaatcggc gaatcggtat
    31201 caatattttt gtcacactat ttgtcgggat gatcgtgatt attgctactg gtagtatttt
    31261 gacagtccgg caattagcag aagcagagga agaaaaagaa aattatcacc tattgactaa
    31321 gctggggatt ccacaaagaa ggatcagacg gatgatcttc gagcaaaacg cacttacctt
    31381 ctttccaccc atgatattag ggatcactca cgcagtattt gcaatcaatg tcttcactca
    31441 gtatataaaa actgccgatt actggttggc ttatcttgtg agtggcttat tgatccttat
    31501 ctatcttttg ctttatgtca ttacgagtca gttatattgt cggattatcg aggaatgata
    31561 tacggaataa gacaagataa taaatagaac taaattttga ataccattta tttggaataa
    31621 gaggttgtga cacgattttt gtcacaacct ctttattttt tatatttttt aaaagtacga
    31681 gaaattataa agaaaaaagc agtgatttga tgtgctattt ttataaaagc tatgtgacaa
    31741 aactgttagt aaaaaaacgg tttctgtaag ctagacacat gtttcttttt gtcaaaatta
    31801 gttacacttt gtgtgaggtt atatgaggag ggaatccaat gaacaaatgg cgttctaatc
    31861 caaaattgaa ttattgggga aaattcatca ttcgaacaat tttttatagt gcagtactgc
    31921 tgttcctgat ttatctgtat cactataaaa atattcaagg aggaactttt atctataacg
    31981 aattttagga ggaagagtat ggaaatcaaa acgattattg aagcaatcga tgattgggga
    32041 ataaatgaac ctgatcgagt tgcttatcag gcggatgaga cgcatacttt tggtgaattg
    32101 aaacaagcat cagacgcact tgcttattat ttagaaaaaa aggtagaagg cgatggacca
    32161 attgtcgttt ttggcaattt ggagtttgaa atgattgtct catttctagg ggttgtcaaa
    32221 tctggccatg cctatttgcc gattgaagag catacaccca aggagcgtat cctatcgatc
    32281 tttcgggtgg ctaaaccatc tatggtcatc agtatcgggg actggataga agatattcct
    32341 tcggttcctg tcataacaaa aaaagaattt caagaaatca agaagatacc agtaggattt
    32401 tatcccagaa atagcgtaaa aggtacagaa aatttttata ttatttttac ttctggtact
    32461 actggagaac caaaaggcgt acaaatcagt catgataatc tagtcagctt tgttcaatgg
    32521 acgatggaag actttgggat caagcctggt atgcatttct tagcccaagc gccattttct
    32581 tttgatttat cggtattttc tatttatcct gcattagttt ctggcggaat gctgaagccg
    32641 ttaaataaag cagttgttca agactttcgt caattattcg cgacattgcc agccttgaag
    32701 ttgaatgtgt gggtttctac tccatcattt atggatattt gcttgatgga acctacattc
    32761 aatggagaaa atgtgccgga attggacatg tttttatttt gcggagaaga attgacgaaa
    32821 aaaactgcag aaagtctact tgatcgattc ccaaatgcac ggatatataa tacttacggt
    32881 ccaacagaag caactgtagc tatttcaagt attcagattg accagcatgt actagatagt
    32941 tatgaccgac taccgattgg ctacgtaaaa aaagacacga aagtttctat tgtccaagaa
    33001 ggtaaacctg tttcaaaagg agaaacgggt gaaatcatta ttgcaggacc aagcgtatca
    33061 aaaggttacc tgaacaatcc cgaaaagaca gctgctgcgt tcttttgtca aaatgactta
    33121 gcaagttacc atacagggga tgcaggtaga ctagatgaat atgggctttt attttatgaa
    33181 ggacgtatgg atcagcaggt caaattacat ggataccgga ttgaactcgg ggatatcgaa
    33241 cattatctgc tccaagacaa ccgaatcaaa caagctgtcg tcgtaccaaa ataccaagga
    33301 acaaaagtac agcagctggt tgcttttgtc gtattagaaa acaaaacgaa acagccggat
    33361 tttcagttga ctaaatctat caaagaacaa ttactagaag ttgtaatgga ttatatgatt
    33421 cctcagaagt tccattatgt cgatctgctg cctcagacag ttaatggaaa aatcgatcga
    33481 aaaaaattaa cagctgaggt gaatcctgaa tgatgttacc tcatatgatc ccttatgcag
    33541 agccgtttta tttttttctt ttagccattg cgcttgtacc gatcgttcta tctttattga
    33601 tatgggaaaa aagattacca gtttatcaat cagcagtgac acttttcttt ttatttgtca
    33661 gctttggagg taattattgg caacagggag cggcgctgat tgggtatgtt ttgtggcaga
    33721 cgatacttgt atggggctat tttgtttatc ggcaaaggaa aaatcagaca ggatggtttt
    33781 ttgccgccgt tattttagcg ataattccac tgtttttaac gaaagtgact cctttttttg
    33841 aacatggaaa agcatcgtta cttggttttt taggaatctc ttatttaacc tttaaatctg
    33901 tacaaatgat catggaaata agagatggca tgatcaaaac atataagcca tttcgttata
    33961 tccagtttct gctgttcttt ccaacaatct cttctggacc aatcgatcgc tataggagat
    34021 ttgaaaaaga tcagctccat ccgcctgttc gtgataaata tgttgatctt ttgggaaaag
    34081 gcgtccaaaa tatttttgtc ggttttttat ataaattcat tatcggttat tattttggtc
    34141 aagtactgct tcctgtagtg ggacgcattg ccttacagca tggtggtatt tcatgggcat
    34201 tagtaggtta tatgtacgtg tacagtatgt atttgttttt tgattttgct ggctatagct
    34261 tatttgcagt cggtacaagc tacatgatgg gatatcagac accaatcaat ttcaataaac
    34321 cttttttatc atggaatatc aaagaattct ggaatcgttg gcatatgacg ctgtcctttt
    34381 ggttccgtga ttatgtatat atgcgattga tgttcttcat gatgaagaaa aaatttttta
    34441 aaagcaagat cgtaatgtca aatatcggtt attttgcttt gtttcttcta atgggtatat
    34501 ggcatggatt gacgtggtac tacatcgtat atggactgta tcatgcttgt ctgatctgtc
    34561 tgacagatgc atggatccga tttaagaaga agcataaaga gcagttgcct tctaacaaat
    34621 ttacgcatgg cttagccgta tttttgacat ttcaagcagt atgttttagc ttcctgattt
    34681 tttcaggatt tttagataaa ctttttttta gctaacgaat agtgacatat taatttatat
    34741 aacaaaggag aaaaggaaaa tggaagaaca aattttatca atcttagaag aaataacagg
    34801 aacagatgaa gtaagaaaaa atcgtgatat tgatctattt gatgaagggt tgcttgattc
    34861 tttagcatct gttcaattat tagtcgagct agacggagag ctggatatcc aagtccctgt
    34921 atctgaattc gagcgagagg attggagcac accgaataaa atcattcaac aagcaactgc
    34981 attgaagggt tgagtatgaa aaagaaactt tttgctattt tcggcccggt tgttttagct
    35041 gttggcttac tgtttgtgtt ttttacctcc tcctatcaag tagatatctc taatccagaa
    35101 acgattagac gtgcctcttc ttccatgtca gtgaacgtgt tgaaaggaga tgcgataaaa
    35161 aattatgcat tatctgaaaa acaatatatt cctttttttg gctcaagtga attaagcaga
    35221 atcagtccat ttcatccgtc tgttctggca gaaaaatacc agcgaaacta tcgtccattt
    35281 ttgctgggag cacccgggac acaatcgtta agccaatata tgatgatgcg ttctgcagga
    35341 gatgctatga aaaacaagaa agtcgtcttt attatttctc ctcagtggtt cgttaaaaat
    35401 ggtgtaaaaa ctgattactt taatacgtat tattctgaac ttcaaacata cgactggttg
    35461 ttttcaatga agaaagtaac gcctgctgat cgctatttag ctagaagatt attaactttc
    35521 tcaaaagtaa aagagaatga tacgttgacg gcgattctgc aaacgatcaa aaaagggaag
    35581 ctacctttac ctgaatcttt gaatcagctg cgcagccaat ggaacatgct gaagagagag
    35641 gatgaagtat tcagcaatat tgggttgaaa gatcgacagc aaaaaatcga tcatgagtca
    35701 aaacgattac caaaacaata ccaagaaaca gaattatcca ttttagcaaa tcaaattgga
    35761 gaacgagaaa cgacgaataa tccattcggg ttgaaaaacg atttttatac gcatcgtatc
    35821 cgagcccacg agccagagct caaacagtca caaaaaaatt gggattatcg tttttcgcca
    35881 gaattttctg attttcagct ggtcttggat caattagcaa aaaaccataa cgaagtattg
    35941 ttcatcatcc caccggttaa cgagaaatgg agcgattata ccggactatc tcaagaaatg
    36001 ttacaaggat ttgccaaaaa gatcaaattt caattaaata gccaaggatt caatcgaata
    36061 gctgattttg tcaatcaagc gggtaccaac tattttatgg aagacacgat ccatctaggc
    36121 tggaaaggct ggttggctgc agatcaacag atcagaccgt ttttagaaga aaatcatatt
    36181 actgctagta agtatcatct tgacgatgct ttttttagca aatcttggca acatcaaata
    36241 ccagataaac tacaattaaa ataaagaaaa caggcgagtg ttgctcgctt gttttcttta
    36301 tttttgaata ttctgaataa aagataaaaa taccaagttt gcctgaacaa aaatcataag
    36361 caatggtata atcacaatat gaatatagaa atgggggaaa acatttggat acaaaagaag
    36421 aacagctact tattgattta acttctgcaa aaggagtacc tggaaatgaa gaagaggtaa
    36481 gagaagtatt cagagaatat gccaaaccat tcgctgatga tattttttat gacggtttag
    36541 gcagtgtgat tgccaaacat ggtgctggcg gaggccccaa ggtctttatt tctggacata
    36601 tggacgaagt tggttttatg gtaacaaaaa taaccgaaaa aggattcttg gaattccaaa
    36661 ctcttggagg ttggtggggg caagtcatgc ttgctcagca agttgaaatc aaaacacgtg
    36721 aagggaaaat cgtccacggt gtgattggtt ccaaaccacc acatgtttta acacctcaag
    36781 taagaaataa accttatgaa ataaaggata tgttcattga tattggcgca tcaagccaag
    36841 aagaagccaa agagtggggc atacgtcctg gagatatggt cactccatat attgaataca
    36901 aacgaatgaa tggcagtaaa tatttactgg caaaagcatg ggataatcgt atcgggacag
    36961 ctgtttctct gcgagtatta gagaatctat ctaaagaagc acacccaaat gttttattcg
    37021 caggaagcga tgtgcaagaa gaagtaggct tgcgaggtgc tcgaacaagt acacaccttg
    37081 taaatcccga tattgctttt gctttagata ctggtacagc aggagacact ccaggaatga
    37141 caccaaaaga agcagattcg attttaggaa aaggcccaca gattttgatt tttgatgcat
    37201 ctatgatccc gcataaaaaa ttattgaatt ttgttattga tgtagcagag gaaatggata
    37261 ttccattcca atatacagtg atttctggcg gaggaactga tgcaggacaa atgcatttga
    37321 caagagatgg tgttccgtct gtagccatta ccgttcctgt tcgttatctg cattcccata
    37381 catcagtgat tcatgaagat gattatttga atacagtgaa actagtcaca gaattagtga
    37441 aacggcttga tagtgaaaca gttgaaaaaa ttcggagtta tgaataatga ataatagaat
    37501 acctatttct gaatatcgga aaaaagcacg agtttctttg gaaaatcaat ggggaatcaa
    37561 tacttggctt gttttcttat ctataatatt tacgagcgtc atccagaact tttttgacgg
    37621 tttcagtcct tttccggaag aaagcatgca gcagcactta ttgagttttc tagtcgataa
    37681 tttactttta ttcgcattca actatgcatt gttttacatt gcgctagtag ttgtacgggg
    37741 tggcagacca aaaagtaacc ttttattttc agttttccaa agaaagtatt ttggttcgat
    37801 acttggcatc aatttgatca attcagtcat caactggcta ctcaacagtt taatctttct
    37861 gcccatgttt ttaatcagcg gagtgagtac ttatatgcag ctgcttttca gttcaaatgc
    37921 ggtacctacg ggtgtcatgc cagaagattt tcttgatatc agctttgttt tatcgatgat
    37981 agggatgatt tttgtttcag cactcatcat gagtattgtt ggcggtttat tccaatttgc
    38041 tgcatggaca aagttcgact atcctgattt gtcggttatt caatgtttga aatatggctg
    38101 gtttttattg aaagatagaa ttggtacgta tattttgttg cagctgtctt ttatcggctg
    38161 gtatttgcta ggagcaatcg ctttgttcat cggtcttttg tgggtcattg tttatgtgaa
    38221 tgtgtcagtt gcagaatttt atgaacaagc acggattgaa aaaggaggtc cagacgccta
    38281 ttttacttag catgtaagca gtttattcat caaaaaaata acttttgtgg taggataaat
    38341 ctttgagggg gaatgtacga tgaaattaac tgttttagga tgcttaggag cgtatcctta
    38401 taaaaatcaa gggacaacag gttatttgct tcaatctgac gggttcaatt tgttgatcga
    38461 tgcaggtagt gctacgctga tcaaattaca agaacacctt gatccacttg atctagatgc
    38521 tgtgatcctt agccattatc atcatgatca tattgctgac cttggtgtat tacagtatta
    38581 ctggcaactt catcctaaga gaaatccgga gacattgctt tcaatttatg gacacacaaa
    38641 agattccatt cactttgaag aattgacgtt ggaaaatgtc tcaaaaggac atccttattt
    38701 tgaagctgaa gaattaaaaa ttggtccttt tctgattact tttatggaaa caatccatcc
    38761 agtagtatgt tacgccatgc gtatcgtaga agaaacaacg gggaaagtat tcgtatttac
    38821 aggtgattca ggttatttgg aaagtttcgc tgattttgcg aaagatgctg atttgtttct
    38881 agcagatacc tatctttttg aaggaaatga acatcatcat gcccatttca cttcaaaaga
    38941 agctggagaa cttgcgaaat cagctaacgt aaagaaactt gtacttacac acctacctca
    39001 gtttggtgat ttagaacaat tgaaaaatga agcgcaaaag gcagcaggca aagggattga
    39061 tatagaactt gctgcagttg acaaagtgtt taacatttag gaggaagaac atgatttttg
    39121 taccaaatga aaaccacgac ccaagaatca atttagcaat tgaacaatat ttgctgcaag
    39181 aaatgccttt agatgaacct attttgctct tttatatcaa tgaaccttca atcatcatcg
    39241 gcagaaacca aaatacgctt gaagaaatca atcgagatta tgtggaagaa aatggcattc
    39301 atgttgtccg tcgcttaagt ggaggcggag ctgtctacca tgattttgga aacctgaact
    39361 ttagttttat catgcctgat gatggggatt cattccgtga ctttgcgaaa gtaacacagc
    39421 caatcatcca agcacttcat gaattgggtg ttgctggggc ggaattgaaa ggccgaaatg
    39481 atctagtcat tgatggcatg aaattttctg gcaacgcgat gtatgctacc aacggtcgga
    39541 tgtttgctca tggaacaatt atgttcgact ctgatatcaa tgaagtagtc aatgcattaa
    39601 aagtaaaaaa agacaaaatc gaatcaaaag gaatcaaatc gatccgttct cgtgtaacga
    39661 atatcaaacc atttcttcca gaagaaaagc aaaaaatgac aacagaagaa tttcgtcaag
    39721 aaatcttgtt gaaaattttc ggcgtagagt cagtagaaga agtaaaaacg tacgaattga
    39781 ctgatgaaga ttggaaaaaa atcaatcgaa tctctgagga atattatcga aattgggatt
    39841 ggaattacgg gaagtctcca gatttcaatt tctctcgcca aaaacgtttt tctatcggtt
    39901 cgattgaagt ccatttgaat gtctcagaag gaatgatcaa agaagcaaaa atctttggcg
    39961 atttcttcgg actaggagat atctctgacg tggaaaaaaa attggttgga cagaaatatg
    40021 ataaagcatc tctatcaaaa atagtcagcc aaattgatgt gaagaaatat tttggtgcga
    40081 tagaaccaga agaattgctt accttacttt attgaaaata aaaaatagcg gaatccacat
    40141 atatggattc cgctattttt tatacaggat tcgggtaatt tcgagagagt tgaacagtga
    40201 tccgatcaat gaaagtatct ttttcctgcg ttagtgttcc tgtgtaacga tccgctacgt
    40261 ctttcatcat cattaagtca aaacgttcct gatcatctaa ttcttctgtg atgcctcgac
    40321 taaataggtt tgtatcgtaa tgtgcactat gttcgcaaca aatgacgaaa tgttcctctt
    40381 gttcgtgtat cgacaaataa atttttcgat acagtggatt ttcgatggca tgagtttctc
    40441 taaaggaatg ctctaaagcg tggatcaaaa gttgagccag atcgtcatcg ttgataggaa
    40501 gttcttcggg aagatctgct tggaaggtta tctgaatatt ccgtttggca gctaacttct
    40561 ggtaacgcgc taagataaga tttgtcacct ggtggttgca aaaagaacac agcatttcga
    40621 aattttttgc ttcttctaat atcttttcca catagttttt agcttttgtt tggtccgtat
    40681 gcattaaatc ttgcagattc tccatctctt ggacaaattc caggcgaagt gtatttactt
    40741 cttggatatg ctgtcgaagc tgctcataat gttgctccaa caaatcattt ttgattttta
    40801 agaaatctac ttgtctccga tctttgtcgg tatttaagat gtgatcgatt agattgtagc
    40861 tgataataac gattagaatg ataatatata aaatcgtaga ccaattgatc gttgtttgat
    40921 agatacctaa tgccgtttgg atgtaaatca agcaatggat aatggctacc aagacgatcc
    40981 aaggtgtaca agctacataa aaacggttat tcttatatgc ttcgccaatg catgcaagtg
    41041 aagtgactaa agtgcttata acactagcaa tattagcaat cggcattaat tcaggaagtt
    41101 ctgcaggagt aaaagttgca taaagcagaa cgccgataaa aatcaaaagt tggaacgtac
    41161 aaaaaattcc gtaccatttt ttatagtaaa acattcggct ccaatagtag cagatgatga
    41221 aagctgacgt taagataata aataaatgag ataaaaaaga atgaacgaat ggttcaaaaa
    41281 gaataccact taaaatatct tctgatacag cttcaaatcc taatgccaaa gcaaagcaac
    41341 ttaagagaat aagtgagtat tcaagacgtt tttgtttata catcaagtag atcgtataga
    41401 aaaaggtccc tatagcgata aaaacagcga ttatcatcgt caatacttgc ggcactgatt
    41461 gtgaataaat aaaactaatc aatggtccag ttgttcctag ataaactttc ggtggcagtc
    41521 cagcatagtt ctgataagga gatgagactg taagggtcaa tttttttccg atataatttg
    41581 ctggtaaatc tatcacagcc aatgagagtc cgggattgct ttcttcctga taatctttcc
    41641 gttggtataa aatcttttcg cctagatata cagtaatcca ttgatggttt cctttgatta
    41701 gtaaatttgc ttcgtccaac ggtttttcaa gtgtcgtttc cataatgaat gtttcgtctt
    41761 tatccacggt aggaatcgta cgagtgtact tgctgtcaaa cttaaaatca ggatcatctt
    41821 ttgtatagaa aacccacgaa tcttttattt ctttcatttg attgctgtcc gcaggagaaa
    41881 aaataaaata aaagaaataa gcaatt
//
LOCUS       DACDKM010000015        41903 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20834_2016
            SAMN12640370-rid9891663.denovo.015, whole genome shotgun sequence.
ACCESSION   DACDKM010000015 DACDKM010000000
VERSION     DACDKM010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640370
            Sequence Read Archive: SRR10040891
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 41903)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 41903)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742835.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742835.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:04:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,012
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 29 of 75
            Pseudo Genes (incomplete)         :: 33 of 75
            Pseudo Genes (internal stop)      :: 19 of 75
            Pseudo Genes (multiple problems)  :: 5 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..41903
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640370-rid9891663.denovo.015"
                     /isolate="E20834_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            83..964
                     /locus_tag="IV334_000781"
     CDS             83..964
                     /locus_tag="IV334_000781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP8942874.1"
                     /translation="MAEIVKVTHLKKSFGRFEALKDVSFTVHSGEVVGFIGPNGSGKS
                     TTIRTLLGIIKKDGGTATIFGQDVWEDSLSIHERLSYVPGDISLWGNLTGGAIIDLFM
                     KLHGKGSAQKKDELIQRFSLDPKKKAKNYSKGNRQKVGLIAALAVDSDLYLFDEPTSG
                     LDPLMETIFQEEVEKLKEQGKSILLSSHILSEVERLADKVVIIREGTVVEEGTLDELR
                     HLTRSTITIETEAENSHLAQLNGVHDFVQKGHLAQFAADNEAFDVILAEASKLGVKKF
                     ESIPPTLEDLFMRHYEG"
     gene            977..2587
                     /locus_tag="IV334_000782"
     CDS             977..2587
                     /locus_tag="IV334_000782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetronasin resistance protein"
                     /protein_id="HAP8942875.1"
                     /translation="MTDKFSRWSTLFSQNLSRDWKKILIWLVGVTAFSGGFVPAFEEL
                     TSGQGLAGLYETLQNPAMIAMVGPTPASNAAEYTLGAMYANEMLLFCSLFAAVISVLH
                     VVSQTRKKEELGLIELIRSFRVGRQANSLAVIAESILINLLLAISISCTMIFFQADSI
                     TAEGAWLFGLSVGSMGILGSAVALFAAQLMPSSASSNGLSLAFLGGLYLLRAITDVAD
                     SSLSMWNPLGWSYLTYPFTTNNWIPLLYLFLFSLILIISSFVLEEHRDLNTGYIPERE
                     GKPYAKRSLLSLRGLLFYLNKGMTISWLITFGLMGAAYGSIYGNMQQFLESNDLMKQM
                     FIQSGVSIEKNFTSTIMVVLICLAAILPIASINRLFTEETHGRMNQLFATKISRNQLY
                     WTNMLLAILFAILAILISSGALGLSALASMKDAKDMDLLMFLKAGFNFLPAVLVFIGI
                     SGFLFGWLPRMGKLAYIYLAYSFALNYFGGILDLPEWFSKTAVPSWVPRMPNDSFDLS
                     VFLTLSCISLALFVLGSIGYRRRDLLEG"
     gene            complement(2664..3323)
                     /locus_tag="IV334_000783"
     CDS             complement(2664..3323)
                     /locus_tag="IV334_000783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HAP8942876.1"
                     /translation="MSHDHLCVSFVPLFNHLVQQDQEKVHALVKHHYVEKGEQILAPN
                     GEPQLVIVASGSMKVYQLSASGKEQLLRVIEPGGYEGESQLLGVPNESLFGEALEKTE
                     ICILRQKDFSELLLHYPALSLKLLAINVEKLTKVQQQTTFLMMEKVEERVAVYLLDLA
                     KATGKDHFFLPMKMKELAAFIGTTPETLSRKFKLLEENGYVKRKNREVWIIDKEQLED
                     L"
     gene            complement(3606..4271)
                     /locus_tag="IV334_000784"
     CDS             complement(3606..4271)
                     /locus_tag="IV334_000784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8942877.1"
                     /translation="MELGKSDYQLFDRPIYAFKQLKESHPTDKIEQIKKEYKEHWQKW
                     KEIQLQTAALLPDMYGMSKPKIESWTNGWNLRSHFWSAYRSESRQDENACLAVLLNQK
                     QYQIYLMYQHYKSEERYGSIAAYNQLLAILKEWSKTVDIKDYYIWPQPEHELDDHLAL
                     SDYLSDTKKQEELKKAMRDRTFQMGKLFFYPQEIEHVEQITAETLQELAPLYQKLGAE
                     KFQ"
     gene            complement(4418..5023)
                     /gene="dhaL"
                     /locus_tag="IV334_000785"
     CDS             complement(4418..5023)
                     /gene="dhaL"
                     /locus_tag="IV334_000785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit L"
                     /protein_id="HAP8942878.1"
                     /translation="MKLTVQEIQEWLTQFAEAINQNKQYLSDLDTPIGDGDHGNNMGR
                     GVSAYEEAFQADHPETISDTFKVFSMAMISKVGGASGPLYGSAFMNMMKATKEVDSID
                     SQEKLGEVIEQGTAGIQSRGKAEADDKTMLDVWLPVTEALKAGNLTKEIIDQAKEHTK
                     VLVAKKGRASYLGERAIGHIDPGAASSAILFSTLLDVINQP"
     gene            complement(5051..6034)
                     /gene="dhaK"
                     /locus_tag="IV334_000786"
     CDS             complement(5051..6034)
                     /gene="dhaK"
                     /locus_tag="IV334_000786"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaK"
                     /protein_id="HAP8942879.1"
                     /translation="MKKIMNDPSNIVEEMLEGLVKSYPELVHRVESSRVVAKNQKAEQ
                     VGLVSGGGSGHEPSHAGFVGEGMLSAAVLGDVFTSPTPDQIQTAIKEADSGKGVLLIV
                     KNYTGDALNFDMAKELAAMDDIEVESVIVDDDIAVENSTYTAGKRGVAGTVLVHKIVG
                     DAARNGASLAELKELGEKVVQATKTIGLALRAATVPEVGKPGFELGEDEIEYGVGIHG
                     EPGYRREKMQPSKVLAKELVTKILDDYSNLPKEAGVLVNGMGGTPLMEQFVFMNDVLA
                     LLEERGVNVVFRKVGNFMTSLDMQGLSLTLIDLTETQWKDSLESNVQTISW"
     gene            complement(6036..6431)
                     /gene="dhaM"
                     /locus_tag="IV334_000787"
     CDS             complement(6036..6431)
                     /gene="dhaM"
                     /locus_tag="IV334_000787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS-dependent dihydroxyacetone kinase
                     phosphotransferase subunit DhaM"
                     /protein_id="HAP8942880.1"
                     /translation="MKKSILLISHSQKVTDGIKEMIEQMQKSEEVQIFSLGGINGEIG
                     SDPMKIVEAVNESSDAASYFVFADIGSAVLNSELAKDMLEEEQQQRYHLVDAPLVEGA
                     FAAAITAGITDDTQQILTEAQNAGKKGWE"
     gene            6585..6905
                     /locus_tag="IV334_000788"
     CDS             6585..6905
                     /locus_tag="IV334_000788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-binding domain-containing protein"
                     /protein_id="HAP8942881.1"
                     /translation="MITTTTNSVEGKAVVAYKGIVFGEVITGIHAFKDLEAGLRNIFG
                     GRSKSYENELMGAREEALAEMAERAKALGADAIIGVKMDYEVLGSDNGMLMVTCSGTA
                     VSTR"
     gene            complement(7017..7886)
                     /locus_tag="IV334_000789"
     CDS             complement(7017..7886)
                     /locus_tag="IV334_000789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015370988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HAP8942882.1"
                     /translation="MGKVMKKRMKPLIWPIAVSSIFLLGQTAFAETSEPAQTEPSTTI
                     SSSGAENGASETEMTSNVPTDNSGETISSSEDTSINSSTSSDQIDHLQDLENKRKELE
                     EALEAAKNKLKEDQAAIDQDLADLKNKVGNDRAQLEKKLAEAKAKAEAVQTDLDGKIQ
                     QAKEFNQQLNQVAQTASGKLSQTQADITAASQAIHGKISEAQTVANDFEAKKADLAAS
                     FQRIKDILHSAGEKSVTSGSTTETVQQTTEPSKSTSQTLPKTNEKTASVSYSLAGAGV
                     LVAAAYGIIRKKK"
     gene            complement(7982..9256)
                     /locus_tag="IV334_000790"
     CDS             complement(7982..9256)
                     /locus_tag="IV334_000790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003745485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HAP8942883.1"
                     /translation="MLRYLFVYYVFFLVSYPILGGFVWFIGVWCYVFLYKHKQKEWVD
                     VPLSVEPFITIMVPAHNEEIVIEDTIEYLMTKLNYHNYEVLVTDDGSTDQTPEILARL
                     MKKYANLRVVRIEKNKGKAHAFNIGLAFAKGKLILSNDADTVPEPDALIRYVNYFIRP
                     GARHIAAVTANMDVQNRTKLIAKSQTVEFSSIVGIIKRTQSAVFGGLYAYSGANTMYR
                     KEALIDVGGFRQDRATEDISIAWDHQLNDWLSVFAPEIIFFMEVPVTLKMLYRQRKRW
                     AKGGTEVWLTNFKKVMLHPFKHIGRTIIFIDQTLSIVWSIFFCISVVLFAGLIGHYVY
                     QGNYEQIYITFTFSFVFICFEMVAGFFQLLASLIVDDRRRKLKYLLFAPLYMLLFWIV
                     NAITIVTTFIPAVKTILGYGSGTWKSPERTKK"
     gene            complement(9282..9791)
                     /locus_tag="IV334_000791"
     CDS             complement(9282..9791)
                     /locus_tag="IV334_000791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8942884.1"
                     /translation="MKKVTREEVASILVKDKYFSKGNYWLKIWQTLVALLGWMIVVLP
                     FIWVFFPLTRPERAKDFSLYAFLSEKKMLYFLIGFFVLIFFSFLITSFVLTRWNNRNL
                     YKKVNKSFEYEDEKLKKRQELLEEVYTERFGTKEERETVRFYSVSEEKNLDTTFIADL
                     YKENGVELK"
     gene            complement(9966..12101)
                     /locus_tag="IV334_000792"
     CDS             complement(9966..12101)
                     /locus_tag="IV334_000792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAP8942885.1"
                     /translation="MVNTQKQLEEAYLKNVYQKLIDKKNELRESMDTAKIEGNQSLKE
                     MAGELRLNFDSYLDNLDTFSMIEMKNREIDQMNIKQKNAENQLAKIERLLKSPYFGKV
                     VVDFLDNEPKESFYIGTANFTDDADENLVYDWRSPIAELFYNNMIGPSSYTVRQNKID
                     TVIKERRQFLLERDRLVKFFDTSVAIQDDVLLEVLGQDETNEMKAITATIQSEQNTII
                     RDVKSQNILVNGVAGSGKTSAIMQRIAYLLYLYRDTMTSDDLLILSPNHRFIDYISNV
                     LPSLGERNPLNLTIIQLVSQLSAEEIEGEEAYFKRITGENVSEQTERLRSKKFIDNLK
                     QSDPLFLDHPNFIRGLTKNGKTVLSKKTIEKIYEKVPAHPKLIDRLQATKKALMSEWK
                     NHLLKQAKSPAVQNQVLSLTEDRQLELFGKLISDDSEQSIVAYARKLLQKKYRKITRQ
                     IEEMAWVDEHQLFERIYEKRYGSAYAWQPTRTVDEAVIILAIRHLLVEKVNVPAFRYL
                     LIDEVQDYTLAQLGLLIELFPKTHFTLVGDENQAIFNSSTTFADIMRCFDDYHLPIHR
                     YDLRNSYRSSGAITELFKTYAVDQEKIDIVSIRPQGKEPEYRSFRSSEELLKILAEIL
                     VSLKGEKAAVITQTEEETQTLQESIKKNSYGLENCQIIPLSLAKGLEFDHVILYPFEN
                     DGDEQRRRRQMYTAISRGMKSIVVLERAT"
     gene            complement(12184..13839)
                     /locus_tag="IV334_000793"
     CDS             complement(12184..13839)
                     /locus_tag="IV334_000793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HAP8942886.1"
                     /translation="MKIVIVGGVAGGMSAATRLRRLMEDAEIVVFEKGPYVSFANCGL
                     PYYLSGEISERENLLVQTPESLSARFCLDVRPNHEVTAIFPENKTVEVVHEGQKHIEQ
                     YDALVLSPGAKPVVPSIPGITEADNVFSIRNVPDIDKVMHALEKQPKRAVIVGAGFIG
                     LEMAENLKRRGLEVMVIEQAPHILPTLDEEMAAFIEKELSHQGVEVITSHAVAGFEDH
                     GKRLRLDDGRTIPADLVILSIGVRPDNQLAVTAGIELGIRGGILVDERYQTNIPDIYA
                     VGDAIVVKQQITGKDALISLASPANRQGRQVADTISGISRRNQGGIGTAIIRTFGMTA
                     ASTGLSERTAKENELSFEVIHVSGKDHASYYPEATDILLKLIFHPETGEIYGAQGVGA
                     KGVDKRIDILATAIKGHLTIFDLPELELTYAPPFGSAKDPVNMLGYAAMNIAEGLSET
                     VQWHELPTELAKGKILLDVRTAEELEKGKFKEAKHIPLNELRDRLNELDSQQEYIVSC
                     HSGLRSYLAERILKQSGYHVKNLDGAFSLYQTVRPEELIYPNK"
     gene            complement(13841..14155)
                     /locus_tag="IV334_000794"
     CDS             complement(13841..14155)
                     /locus_tag="IV334_000794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010730940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HAP8942887.1"
                     /translation="MNLLFQSIKSITVPELKEKLLENPALLDVRTPAEYRGGHIKGAK
                     NVPLQSINRYDGDKEKTVYVICQSGMRSKQAAKELKKSGYDVVNVRGGMNQWFDRTVG
                     GK"
     gene            14325..14585
                     /locus_tag="IV334_000795"
     CDS             14325..14585
                     /locus_tag="IV334_000795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sensitive transcriptional regulator"
                     /protein_id="HAP8942888.1"
                     /translation="MEIDNKNILNRLKRTEGQIRGIQKMIEDEKECMDVITQLSAVRS
                     SIDRVMGMIVADNLKNCFEKPDSNPEEQAAKLEQAIKMIIKK"
     gene            14599..14913
                     /locus_tag="IV334_000796"
     CDS             14599..14913
                     /locus_tag="IV334_000796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur reduction protein DsrE"
                     /protein_id="HAP8942889.1"
                     /translation="MKVVFHIDELEKWSETGKNVKNLIKASPETTIVVSVNGIAITGY
                     LDSANAEFLDLQGVVFHACANAMRANHISESSLPEQVIVVPAGVLDLVELQSQGYAYI
                     KP"
     gene            complement(15643..16197)
                     /locus_tag="IV334_000797"
     CDS             complement(15643..16197)
                     /locus_tag="IV334_000797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HAP8942890.1"
                     /translation="MKLSTDWRKEIQTIPNLLSIFRIFLLPIYLYFVLRQSFYIAGAV
                     IVVSGLSDYLDGVIARRYNQVTDLGKVLDPFADKLTQLFLILSMAWYRPWLWLLFGLF
                     LIKEGFMFVAGLIGLSKNIKLSGAKWYGKVATAVIYVGMILLLLFPELPTLWVRVIFA
                     VITYGLLQSFVLYAVEYRKMFQRK"
     gene            complement(16323..18251)
                     /locus_tag="IV334_000798"
     CDS             complement(16323..18251)
                     /locus_tag="IV334_000798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HAP8942891.1"
                     /translation="MDNEMISQLQFDRIKKEIQARAIGNYSKKRISDMTVSTNLQTVL
                     DRQEETREARLILESSQHVPFMGLPRIDALTEQVKKGLILQPTDLIEYADFLRSSRMI
                     TKFFDKNQYQTPLLFAYSKHLPDLINVEELIDQKIKNNKVSDDASRNLRKVRKQLQLI
                     EKEIQSKLLKFLRHPKNKEMIQEAMIVQKGEYYTIPIKASYKNKIDGTIIDESNKRTT
                     VFIEPTVISKLNEHYQLLKAEEISEEYQVLAALTGAIAENEEVIDLLIETITVLDIIF
                     ARAKFSREINGSTPRINKSEHIIIKQGRHPFLPEHAVPLDVEIGKDYRGLIITGANAG
                     GKTVVLKTVGLLTLMAMFGMQVPAKEGTELAVFDEIFVDVGDHQNLENALSTFSGHMQ
                     NIAAILKKIKRNTLVLLDEIGSGTEPNEGAALAIAIMESMYEQGALIIATTHYGEIKK
                     FAGDHEDFVPAAMAFDREALRPKYQLRVGETGESQALWIAHKMDMSMKLIHQAERYLA
                     EKAYRTQKKEFLPKTAVTNRKENKKERQLFAKGDRIFVNEYQKEALVYEDIGEDTIDV
                     YLDKKIIHVPRQRVRLVRSAEDLYPTGYDLDSLFIDYKTRKRQRDLERGSKKAHKVLV
                     KEMRKRQEERRVNDENSK"
     gene            complement(18557..19273)
                     /locus_tag="IV334_000799"
     CDS             complement(18557..19273)
                     /locus_tag="IV334_000799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin family protein"
                     /protein_id="HAP8942892.1"
                     /translation="MNSDMTQIMGEFIGTLILVLLGDGVCAAVNLNKSKAQASGWIVI
                     AFGWGLAVTMAVYISGFMGPAHLNPAVSLAMAMTGAISWNLVVPFIIAQVLGAFAGAI
                     LVWLSYLPHWNATKDESAILGTFATGPAIRNYPANVITELIGTFVLVLGLLAFGQNEF
                     APGTNVFAVGGLILAIGLSLGGPTGYAINPARDFGPRLAHAVLPIANKGTSDWAYSWV
                     PIAGPMIGAIIAVGVYSLMV"
     gene            complement(19274..21097)
                     /gene="glpO"
                     /locus_tag="IV334_000800"
     CDS             complement(19274..21097)
                     /gene="glpO"
                     /locus_tag="IV334_000800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glycerol-3-phosphate oxidase"
                     /protein_id="HAP8942893.1"
                     /translation="MFSNKTRQDSIQKMQQEELDLLIIGGGITGAGVAVQSAASGIKT
                     GLIEMQDFAEGTSSRSTKLVHGGIRYLKTFDVEVVADTVGERAVVQSIAPHIPKPDPM
                     LLPIYEGEGATTFNMFSVKVAMDLYDKLANVTGTKYENYTLTPEEVLEREPFLKKEGL
                     KGAGVYLDFRNNDARLVIDNIKKAAEDGAYLVSKMKAVGFLYDGDQIVGVKARDLLTD
                     EVIEIKAKLVINTSGPWVDKVRNLNFTRPVSPKMRPTKGIHLVVDAKKLPVPQPTYFD
                     TGKQDGRMVFAIPRENKTYFGTTDTDYQGDFTDPKVTQEDVDYLLDVINHRYPEANIT
                     LNDIEASWAGLRPLLIGNSGSDYNGGDNGSISDKSFNKVVDTVSEYKENKVSRAEVED
                     VLNHLENSRDEKAPSTISRGSSLEREPDGLLTLSGGKITDYRKMAEGALRLIRQLLKE
                     EYGIETKEIDSKKYQISGGNFDPTKLEETVTELAKEGVAAGLEEEDATYIADFYGTNA
                     RRIFELAKEMAPYPGLSLAESARLRYGLEEEMVLAPGDYLIRRTNHLLFERDQLDEIK
                     QPVIDAIAGYFEWTEEEKAQQTKRLEALIAESDLRELKGEK"
     gene            complement(21111..22607)
                     /gene="glpK"
                     /locus_tag="IV334_000801"
     CDS             complement(21111..22607)
                     /gene="glpK"
                     /locus_tag="IV334_000801"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HAP8942894.1"
                     /translation="MTESTYIMAIDQGTTSSRAIIFDKKGRHIGSSQKEFTQYFPQES
                     WVEHDANEIWNSVQSVIAGAFIESGIKPNQIAGIGITNQRETTVIWEKDTGRPIYHAV
                     VWQSRQSAGIADNLKNEGYQEFFHEKTGLVIDAYFSATKIRWILDHVEGAQERAEKGE
                     LMFGTIDSWLVWKLTDGQAHVTDYSNASRTMLFNIHQLDWDQEILDLLNIPRVMLPKV
                     TSNSEVYGYTQGYHFYGSEAPISGMAGDQQAALFGQMAFEPGMVKNTYGTGSFIVMNT
                     GEKPQLSKNNLLTTIGYGINGKVYYALEGSIFVAGSSIQWLRDGLQMLQKASDSEDAA
                     KRSTSEDEVYVVPAFVGLGAPYWDQAARGAMFGLTRGTTKEDIIKATLQSIAYQVRDI
                     IDTMQDDTGIKIPVLKVDGGAANNEYLMQFQTDILNVPIQRAENLETTALGAAFLAGL
                     AVGYWKDTDEIREFYEAGKLFEVQMEEERREKLYNGWKKAVKATQAFE"
     gene            complement(22743..24203)
                     /locus_tag="IV334_000802"
     CDS             complement(22743..24203)
                     /locus_tag="IV334_000802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAP8942895.1"
                     /translation="MKIEALLERKEQLQILILRNLVLQGGTASINGLREHVQLSKASF
                     DQYLEDIELIGRMMEKKVEVQRNEYQALLVLDEDVSLEKILLFLLQESLKFKMLVYLL
                     EHQQVSIVRLATAFNISESSVFRKIKELNQLLEEFGLQIKNGQLYGEELQIRYFYYEL
                     FQYIPEDQRPLFLQNTPEKRPFILGLDRVLETTFTASAEAQIACWLGITKKRLLNEKS
                     TYATLKEKKLLYQSDRLYQAIDPIIVMHLSRTAAELNVYETMMFYSFFVSFSIVDEEI
                     FYQYDLTRSKKLPTAVLDTYIRETMLWHYRPRRLKIKEEKAVGYQISQINNEWFFFVG
                     KIEVYERDRLLEQQQKMLGHSLTQLLAKLQETAVQQLPRKRAEDSELSYLMIQYANVL
                     LMIDFYIAKPVVIGIDLESLPIYRIAFQQYLIRELRGIGGIEIGSYEEGKEYDLVITF
                     CQRNKKQCEYYLSEFASPYDIIRLKRRIETLKKEKN"
     gene            complement(24193..25488)
                     /locus_tag="IV334_000803"
     CDS             complement(24193..25488)
                     /locus_tag="IV334_000803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HAP8942896.1"
                     /translation="MNIVIVGGSFAGIHAAIYLRQLMPTSEIYLIEKQSKLGWIPSGF
                     NLILKGKVTSEKQLSWITKEELTDRYRIHVYTEKTVIGLKEKKVVLAGGKKLDFDRLI
                     LATGSNQKFRNISAESTLIHPVKKIQNVTALQQKIMNAKKIAIIGAGQAGIEIAEGLA
                     SRKKQIHLYESRKSILFRYLDPEMTEPLVKEMKNQGLSLFLEEQVVSLTENETAIVET
                     EKRKENYDLVLLANHSRPDNQMWEEQLTLNDDGTIWVDDYLQTSQKDVYAIGDAIQVT
                     FRPTNEKMYVSLVNNAVRTARNVSKTISGSQTKDQGTYRPVGNQWFGYFLGSVGLTEE
                     ESIFYPQKIETGYVDYKLTAINQSKVKLKFLFDSEKKILGAQLQSKKEMFHLLDQLTI
                     AVEEGWTFEQLEEHELFFQPEYRVPDMVWSKVANTAYED"
     gene            complement(25820..26497)
                     /locus_tag="IV334_000804"
     CDS             complement(25820..26497)
                     /locus_tag="IV334_000804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase
                     domain-containing protein"
                     /protein_id="HAP8942897.1"
                     /translation="MKVLIVLTNVEKYEKIERPTGLWLSELTHFYDVLVKNGIDADFV
                     SPKGGYVPLEPQSVLNMDEIDWAYYEDEDFRNRALGNTLRPDDVDANDYAAVYYAGGH
                     GTMWDFPEAIDLGKVASQIYRNEGLVSAVCHGVVGLLPVTDESGKSILAGRTVTGFSN
                     EEEEANGTTNEVPFLAEDALKEKGANYQSGAAFSEVVQIDGRLLTGQNPQSAHQLGEA
                     VVEALKN"
     gene            complement(26527..27618)
                     /locus_tag="IV334_000805"
     CDS             complement(26527..27618)
                     /locus_tag="IV334_000805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent flavin oxidoreductase"
                     /protein_id="HAP8942898.1"
                     /translation="MSQFDQSITLPSGVQLKNRLMLSPMTTQLSFFNGVITEDEITYY
                     KQRSKGLGAVITGAANVQDIGKGWPGELSIAHDEMIPRLSELAKAIQGEGAKAIIQIF
                     HGGRMTSRATLSGEQPVSASAVAAKRPDAETPRAMSEEEIVETIQAFGKATRRAIESG
                     FDGIELHGANTYLIQQFFSPHSNRREDQWGGSLENRYRFIEELLTTVFSAVEQYAKKP
                     FIVGYRFSPEEYETPGIRFEDTIWLLERLRESKLDYLHVSLNMYDRIARSDKYNDKSI
                     LEVVHNTIQGKIPLVGVGGIRNREDVEQVLKYAELAAIGQQMIVDPDWAEKLLENRED
                     EFVTKPFEEAYKELYLPSPLYNFLKARYQ"
     gene            complement(27719..28153)
                     /locus_tag="IV334_000806"
     CDS             complement(27719..28153)
                     /locus_tag="IV334_000806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAP8942899.1"
                     /translation="MEKPIEKALDECLFFSVKKLDRMLNKLADEAFRRTGLAPTYGFI
                     LLILKEKDGIPQKDIAQMLYSAPSTIARFVEKLEYKGYVKTVSEGRLSLVYLTEQGRQ
                     FAKEVDGSWEELHQSYKAVLGNEASKELAQTMNDATDKLQKK"
     gene            complement(28737..30194)
                     /gene="ascB"
                     /locus_tag="IV334_000807"
     CDS             complement(28737..30194)
                     /gene="ascB"
                     /locus_tag="IV334_000807"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HAP8942900.1"
                     /translation="MKKFPEGFLWGGATAANQCEGAWDVDEKGLSVSDVYTFDSDLPK
                     EKWTDQWHMMTHQQVKAAQDPNSKYYYPKRHGNDFYHHFKEDIRLFAEMGFKCYRMSI
                     AWTRIFPHGDEETPNEAGLFFYDQVFDECLKYGIEPVVSLSHYEMPLYLVTEYGGWPN
                     RKLIQFYVRFAETVFKRYKNKVKYWMTFNEINCVKHHPFVSVGVIEENHPHLEQAKYQ
                     GAHHQFIASALATKACHEIIKGSQVGCMISYQLLVPYSCDPDDIQKTIEQQRTSLFFS
                     DVQARGYYPAYTQRMFEEKGVNLKIEVGDEEILAAYPVDFVSFSYYMSSAVSAHPENW
                     EGVQGNLITGGIKNPYLPSSEWGWQIDPKGLRIALNQLYDRYQKPLFIAENGLGAIDK
                     VEEDGTVQDDYRIDYLKQHIEQMKEAIKDGVDLFGYTSWGCIDIVSASTSQMSKRYGY
                     IYVDQDDEGNGTKKRLKKKSFDWYRQVIQSNGEIL"
     gene            complement(30191..32086)
                     /locus_tag="IV334_000808"
     CDS             complement(30191..32086)
                     /locus_tag="IV334_000808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP8942901.1"
                     /translation="MEKPFSKNYTYLVRRIATALINKGEVKMDYSQLAKDIVRFVGGE
                     ENVSNVYHCATRLRFTLKDNKKADKEKVEQLEGVITVVEAGGMFQVVVGNAVNEVYDV
                     LSKQMKLEDDASSGKRGTEKKGILNSFIDMIAAVFAPTLGVLAGSGLIKGVLALCTSL
                     NLLTTESGTYIILNAAADAFFYFLPIFLAYTAAKKFNTDRFIAMVIAAALVYPTIVSA
                     YSDSITLRFLGMPVILARYTSTVIPAILAVWVLSYIEPKIRKSLHESIRNLLTPFICI
                     IVMVPITLLVVGPIADYASQLIAAGYLAVYNFSPVLSGAVIGGFWQVLVIFGLHWGLV
                     PVMTNNLSFYGRDTLGPACMTAVAAQAGAVLGVFLKTKNKKVKSLSLSAFITALFGIT
                     EPAVYGVTLKYKRPFYIACICGAIFGGVAGAAGAGALAVATRSILSFPIYIGEGFVWL
                     VASYFLAMISSCMLTFLFGYKDEIEEESSKDIVLSTPAAGEIIDLSEVNDPTFASGSL
                     GEGFAIIPTDGKIYSPVNGEVSTVFPTKHAIGVVSEEGAEILIHIGIDTVNLNGKYFQ
                     SAVSDGKKVRKGDLLMEVDLQELIKEGYDPTTMVIVTNSSRFKNIITSHKKNNQTRKL
                     ASLGGSI"
     gene            complement(32144..33919)
                     /locus_tag="IV334_000809"
     CDS             complement(32144..33919)
                     /locus_tag="IV334_000809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HAP8942902.1"
                     /translation="MFITKRRCKQMDLNKRQLQILVVLRNKNHWITSEELAEALGTNK
                     KTIQTEIKNMLAIYEKKIIIQSNKRSGYFLESIESETKQQIIQEVTKHKIYSSMDFRA
                     STIVTYLLFQKGYVSMQKIADIFFLSKTAVSLQIKTILRWIERNPKLELEVSSTKGLK
                     IIAEENSKRIFLSLVGTETVIQHARLPQQISTIFSNIMPTVQKTLKEVLISYNYIVSG
                     EDFIRFSRYLVLTILRREELLEKTNRYTLFIPMVETLTKKLSSELPYSFSNQEKQAIE
                     NRLLELNYLMINDEKNKEIEKNLRAFEQTIIRFLDLPVSLLFHETEFLLTHIKQMKTR
                     MDAGHNTMNHFVQKIISRYPLEIYLIRRFFPIHFQLRPNLAESAYFVLYLAEALNPFR
                     KQGEILIVSNQPISILNTLKKQIQQSMNMWIKECRIEPVYLFKNQSNKITEYDAILTT
                     EQELIFEWDDLIYLPSVLDEFESWEIYQILREKLTIVEEKKKTKLLDHYYKEENQIKI
                     HKKIDSIQQLISPSSDLVMLPLNAETLLCCLIKSQVETKITEYQLEIPLIHQQRKIKR
                     VFFVSFDYKRNDILVFFQAVSDLLG"
     gene            complement(33969..35465)
                     /locus_tag="IV334_000810"
     CDS             complement(33969..35465)
                     /locus_tag="IV334_000810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HAP8942903.1"
                     /translation="MDQEIFPENFLWGGAVAANQCEGAWLEDGKLPNVTDTLIGIMNQ
                     HPSIQWNEEKKIWEIALDESLHYLSHEAIDFYHRFEEDIRLLKELGLKAFRTSISWAR
                     IFPRGDEQKPNEAGLVFYDRLINTLNRYDIEPVITLSHYETPLALVGEYGGWQNRKLI
                     DFFEFYAQTVFERYQGKVKYWMTFNEINNAFRMPYAAAGLVTFPARDKLEPIATLTKK
                     VIYQACHHMFLANARATQLLKKIDPNAKMGIMCSFSALATYAYDCDPENVFGSLQFKR
                     NSWFFSDVMCRGHYPAYIYRTWKEEDCAPVMLDGDEQCLQANTADYIAFSYYRSAVYK
                     KEAEMRVDTGGANGFDNPFLKEKSPEPWSWPIDPLGFRYVMNELTDRYELPLFIVENG
                     IGLDEAPDELNRIHDPKRCEYLKIHLQEIAEAIKDGCQIIGYLWWGPIDIVSAGTGEM
                     RKRYGFIYVDRQNDGSGSFRRLKKDSFEYYKQIIQSNGKNLEYRSFFK"
     gene            complement(35659..37281)
                     /locus_tag="IV334_000811"
     CDS             complement(35659..37281)
                     /locus_tag="IV334_000811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HAP8942904.1"
                     /translation="MEQKWWHHSVVYQIYPRSFQDSNQDGIGDLRGIISRLDYLAYLG
                     IDAIWLSPVYESPNDDNGYDISNYQAIMAEFGTMEEMEELIEEAKKRNIRIIMDLVVN
                     HTSDEHPWFIEARKNKENPYRDYYIWRDPVNGKEPNRLRSIFSGSAWQLDEKTGQYFL
                     HLYSKRQPDLNWKNKQVRQEIYQMMNFWIDKGIGGFRMDVIDMIGKEPDKEITSNGPK
                     LHEYLQEMNQATFGDKNLMTVGETWGATTEIAKLYSNPKRNELSMVFQFEHIGLDQQE
                     GKDKWDLKPLSISELKKVLSKWQTSLGDEGWNSLFWNNHDLPRIVSRWGNDDVYRKES
                     AKMFAILLHMMKGTPYIYQGEELGMTNCPIDDIAEAKDIETINMYNERISSGFTKEEI
                     LESINAKGRDNARTPMQWNAREHAGFTTGIPWLRVNPNYKEINAEEALADQDSVFYMY
                     KKLIDLRRKHDIIVWGDYELVEKTPEHVFAYSRIYEDQQWLVICNMSSEKQTFTVPRE
                     ALRVIVSNYPLKQLPDGKVDLRPYEAFVLEVK"
     gene            complement(37730..39043)
                     /locus_tag="IV334_000812"
     CDS             complement(37730..39043)
                     /locus_tag="IV334_000812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HAP8942905.1"
                     /translation="MKEKILSYFEIEKLNTTVKREVLAGFTTFISMAYILFVNPTVLG
                     ASGMDEGAVFTATALASAIGCVLMGVLARYPIGTAPALGINAFFAYSVCLGMGIPWET
                     ALAGVFVASLIFILITIFKLREMIIDAIPADLKYAISGGIGLFIAFLGLSEGGIIVSN
                     ESTLVGLGSLNVGSTWLTIFGLVVTAILLVRRVPGGIFIGIAATTILGLVTGLIPMPS
                     EIVAAAPSLKPTFLVALKHVGDINSLQMWVVVLTFLLVTFFDTAGTLVGLANQAGFMK
                     DNKMPRVGKALASDSTAMLAGSLFGTSPVGAFVESSAGIAVGGRSGITAITTGIFFLL
                     GLFFSPLLSVVTSQVTAPALIMVGVLMAQSLRQIEWGNLEIAIPSFLILIGMPLTYSI
                     SDGIALGFIFYPITMLAAKRGKEVSPIMYGLFFVFVGFMWILNVK"
     gene            complement(39288..39512)
                     /locus_tag="IV334_000813"
     CDS             complement(39288..39512)
                     /locus_tag="IV334_000813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8942906.1"
                     /translation="MDLMGRLEGYLKYRRAKHSEKLRAKNLKSGKKKTKGWGKMVDTG
                     FGGLNNLNPVDTKEFIKEEQKRNGKIKSHY"
     gene            complement(39532..39900)
                     /locus_tag="IV334_000814"
     CDS             complement(39532..39900)
                     /locus_tag="IV334_000814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase"
                     /protein_id="HAP8942907.1"
                     /translation="MIQKTRVMLYVNDVEMICRFFVEKIGAEISETIELPEEFKSIVL
                     SISKELELGVFPKVFVQKFSPEVLGPPPSLVFFTDEFETIYENMEEPGEITDNNGILT
                     FNFSDPEGNYFVIGKAESSD"
     gene            complement(40098..40808)
                     /locus_tag="IV334_000815"
     CDS             complement(40098..40808)
                     /locus_tag="IV334_000815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8942908.1"
                     /translation="MKKVIVDTNFWEVFPEATIEILSVNGIDNHVTKESEETYHQLLN
                     EAAKEARNYLTEETFSQNEVIAQWRQAFTTFKTKKGARSSIEALLKRANQEREFFPIN
                     PLVDIYNSISLSFGVPCGGEDIEKIAGNLHLGKAQGGESFLPLGADSDAPALPEEIIY
                     YDEQGAICRCLNWREAQRTMLTEETQAAVLVIESINAEQAARGAQAIEALKEKIDQTF
                     GVTSVRHTLTKDESEAEL"
     gene            complement(40826..41323)
                     /locus_tag="IV334_000816"
     CDS             complement(40826..41323)
                     /locus_tag="IV334_000816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP8942909.1"
                     /translation="MTPSDVDLLQQISIETFTDTFGKDNSPEHLADYLTQAYALEKLT
                     EELKDKETDFFLVSIDQEPAGYLKLNTGTSQSEEMADHFLEIERIYVRKKFKHQGIGT
                     FLLDTAIEKAKEKQKTAIWLGVWEHNDVALAFYRKKGFIKYSEHVFYMGDDPQTDYLF
                     IKQLF"
     gene            complement(41360..41812)
                     /locus_tag="IV334_000817"
     CDS             complement(41360..41812)
                     /locus_tag="IV334_000817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003331879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAP8942910.1"
                     /translation="MAEILRDIGMIARALDSISNIEFKEVDLTRGQYLYLVRICENPG
                     IIQEKLAEMIKVDRTTTARAIKKLESNGMIERLEDKENKKIKKLYPTKKGAEIYPFII
                     RENNYSNAVALNGLSDEEAKQLEYLLNKVCKNVSEDWNFVKKGNKRIY"
BASE COUNT        11757 a         8926 c         7174 g        14046 t
ORIGIN      
        1 tttataaaaa ggtataaaac ctttttatta aactatttaa tcttgtttag tatatatttt
       61 tagaaaaagg agtggggttt ttatggcaga aatcgtcaaa gtcacacatt tgaaaaagag
      121 ctttggtcgt ttcgaagctt taaaagatgt ctctttcacc gtacattcag gtgaagtcgt
      181 tggatttatc ggaccaaatg gttctggaaa atccacgacg attcggacac ttttagggat
      241 tataaaaaaa gacggcggaa cggcaacgat ttttgggcaa gatgtatggg aagatagttt
      301 atctatccat gaacgtcttt cttatgttcc aggcgatatc tctttgtggg gaaatttgac
      361 tggaggagcg atcatcgatc tcttcatgaa gcttcatgga aaagggagtg ctcaaaaaaa
      421 agatgaactg attcaacgtt tctctcttga tcctaagaaa aaagcaaaaa actattcaaa
      481 aggaaatcgc caaaaagtgg gcttgattgc tgctttggct gttgattcgg atctctattt
      541 atttgatgag cctacttctg gtttagatcc tttgatggaa acgattttcc aagaagaagt
      601 tgaaaaattg aaagaacagg gcaaatccat cctactttct tctcatattc tcagtgaagt
      661 cgagcgacta gcggataaag tcgtgatcat acgcgaagga acagtcgtag aagaaggaac
      721 actcgatgaa ttgcgtcatt tgactcgttc gaccatcact atagaaacag aagcagagaa
      781 tagtcactta gcacaattga acggtgtcca tgactttgtg caaaaaggtc acctcgctca
      841 atttgctgct gataacgaag ctttcgatgt gattttagct gaagcgtcca aattaggtgt
      901 gaaaaaattt gaatctattc caccgactct tgaggattta ttcatgagac attatgaagg
      961 atgaggagga agcgaaatga ctgataaatt ttcacgctgg tccacccttt tttctcaaaa
     1021 ccttagccgt gactggaaaa agatcctgat atggctagtc ggtgtcactg ctttttctgg
     1081 tggttttgtc cctgcgtttg aagaattgac ttccggacaa ggattagctg gattatacga
     1141 aaccttgcaa aacccggcaa tgattgcaat ggtcggacca actccagctt cgaatgctgc
     1201 tgagtatact ttaggagcga tgtatgcaaa tgaaatgttg cttttttgca gtttgtttgc
     1261 tgctgtgatc tctgttttac acgtggtgag ccaaacgaga aaaaaagaag aacttggtct
     1321 gattgaactt attcgttcct ttcgagtcgg acgtcaagca aactctttag ctgtcatcgc
     1381 tgaaagtatt ctgatcaatt tgcttttagc aatatcgatc agctgtacga tgattttttt
     1441 ccaagcagat tcgatcactg cagaaggtgc ttggcttttt gggctatctg taggtagtat
     1501 ggggattttg gggagtgccg ttgcactgtt tgctgcacaa ctgatgccaa gttcagccag
     1561 ttcgaatggt ctgtctttag cgtttttagg tggactttac cttctacgag caataacaga
     1621 tgtagctgat tcctcccttt cgatgtggaa tcctttaggc tggtcttatt tgacctatcc
     1681 atttacaact aacaactgga ttcctttgct ttatttgttt ttgttcagtc taatcttgat
     1741 aatcagttca tttgttttag aggaacatcg agatttgaac accggctata tccccgaacg
     1801 tgaaggaaaa ccgtatgcta aacgttctct tttatccttg cgtggtttat tattctatct
     1861 aaataaaggg atgacaatca gttggcttat cacttttggt ttgatggggg cggcatacgg
     1921 ctctatctac gggaatatgc aacagttttt agaaagtaac gacttaatga aacagatgtt
     1981 tatccaatct ggcgtctcaa ttgaaaaaaa ctttacaagc acgatcatgg tcgtcttgat
     2041 ttgtctagct gctattttac caattgctag catcaatcgt ttatttacag aagaaaccca
     2101 cggacgtatg aatcagctgt tcgcaacaaa aatttctagg aaccaacttt attggaccaa
     2161 tatgttacta gctattctat ttgctatatt ggcaatcctg attagttctg gtgcactagg
     2221 gctttctgct ttggcttcga tgaaggatgc gaaagacatg gatctattga tgtttcttaa
     2281 agccgggttc aatttcctgc ctgccgtttt agtgtttatc ggaattagtg gctttctatt
     2341 cggatggctt cctcgaatgg gtaaacttgc ttatatctac ttagcttact cctttgcact
     2401 caattacttt ggcggaattt tggatttacc agaatggttt tccaaaacag ctgtccctag
     2461 ctgggttcca cggatgccaa acgattcttt tgatctttcc gtgttcctta cgttatcatg
     2521 tatcagcctt gcactgttcg tccttggttc tattggttat aggcgaagag acttactaga
     2581 aggataataa aaaagcatgt acaatttcat gctagcctcc aaatctgggt caaaaatcca
     2641 acatttggag gtcatttttt tatttacaag tcttctaact gctctttatc aatgatccag
     2701 acttctcgat tctttctttt tacataacca ttctcctcta ataatttaaa ttttctagat
     2761 aaagtttcgg gggtcgtacc gataaatgca gcaagttctt tcattttcat tggcaggaaa
     2821 aagtgatcct tccctgtcgc tttagctaag tcaagcagat agacagcgac tcgttcttct
     2881 actttttcca tcatcaaaaa agttgtctgc tgctgtactt ttgttaattt ctcaacgttg
     2941 attgctaaaa gtttcagact aagggcggga taatgcagta agagttccga gaaatctttt
     3001 tgtctcaata tgcaaatttc cgttttttct agtgcttcgc caaataaaga ctcatttgga
     3061 acgcctagca actggctctc tccctcatat ccgcccggct ctattactcg taaaagctgt
     3121 tcctttccgc tagcagataa ttgatacact ttcatactgc cggaggccac gatcactaat
     3181 tgaggttctc cattcggcgc aagaatctgt tctccttttt ccacatagtg atgtttgaca
     3241 agtgcgtgta ctttttcttg atcttgctgt actaagtgat tgaacaaagg aacaaatgaa
     3301 acacataaat gatcatgact cataatctct cctccttcta aacagctatt tctaataaag
     3361 aaacataact tcccttattc caactgataa ttttctttta tcatagcata aacgtcaagc
     3421 gaaccagata ccagcaaata gagtggattt ctttttgtcc cttgctttat tttccttctt
     3481 ttgagacctt aagtaattga ttcctatcaa ttttttaaat atttactttt tgaataatca
     3541 aaaacaaacc ttcactcgca agccgagagt aaggtttgtt tttcgcattg cttatttcgt
     3601 gttgtctatt gaaatttttc cgcacctagc ttttgatata gtggtgctag ttcttgtaag
     3661 gtttcagcgg ttatttgctc aacgtgttcg atttcttgcg gatagaagaa tagtttaccc
     3721 atttgaaagg ttcggtctct catcgctttt ttcaactctt cttgtttctt tgtatcggac
     3781 aaatagtcag acaaagctaa gtggtcgtca agttcatgtt ctggttgcgg ccatatgtaa
     3841 taatccttga tatcgactgt tttgctccac tctttcagta tcgcaagcaa ctggttatac
     3901 gctgcaatgc taccatatcg ttcttcgctt ttataatgtt ggtacatcaa atagatttgg
     3961 tattgttttt gattcaacaa aactgctaga caggcattct cgtcttgacg agattcgctt
     4021 cggtacgcgc tccaaaaatg actgcgtaaa ttccagccat tcgtccagct ttcgatttta
     4081 ggtttgctca tgccgtacat atcaggcaaa agtgcagctg tctggagctg aatctctttc
     4141 catttttgcc agtgttcttt atattctttc ttgatttgtt caatcttatc tgttggatgg
     4201 ctctctttga gctgtttgaa tgcgtagatt ggtcgatcaa acaactgata atctgatttt
     4261 ccaagttcca ttgtttcgcc tctttccgtt atcttttgta ttctacctga attttgcgat
     4321 atggacaaat agaggttgtg aaaaagctgt tctgcatcaa gtcataagaa cagccaaaca
     4381 aaaaactgcg atgagcatca ccgcagtttt attgaactta aggttgattg atcacatcta
     4441 aaagcgttga aaataaaatg gcactagaag cagctcctgg gtcgatatgt ccgattgcac
     4501 gctctcctag ataggaagct cgtccttttt tagctaccaa gactttggtg tgttcttttg
     4561 cctggtcaat gatttctttt gttaaattcc ctgccttcaa tgcctctgtg actggcagcc
     4621 aaacatctaa catcgtttta tcgtcagctt ctgcctttcc tcggctttga atacccgctg
     4681 ttccctgctc gatcacttcg cctaattttt cttgagaatc aatagaatcg acctcttttg
     4741 tcgctttcat catgttcatg aatgcagatc cgtataacgg accagaagcc ccaccgactt
     4801 tcgatatcat cgccatagag aatactttga atgtatcgct gattgtttca ggatgatcgg
     4861 cttgaaaagc ttcttcataa gctgaaaccc ctcttcccat gttatttcca tggtctccat
     4921 cgccaatcgg ggtatctaga tcactgagat attgtttgtt ctgattgatt gcctctgcaa
     4981 attgcgtgag ccattcttgg atttcttgaa cggttagttt catattatca cccttttatc
     5041 ttgagatttt ttaccatgaa atcgtctgta cattgctttc taaagaatct ttccattgtg
     5101 tctccgtcaa gtcgatcagt gttagagaaa gaccttgcat atctagtgag gtcataaaat
     5161 taccgacttt tcggaaaact acattgacgc ctctttcttc caacagagct aaaacatcat
     5221 tcatgaacac gaactgttcc atcaaaggcg tcccgcccat tccgttgact agtacacctg
     5281 cttcttttgg taaattcgag taatcatcta aaatcttagt aacaagttcc tttgccagta
     5341 ctttagaagg ctgcatcttt tctcgacgat agcctggctc accgtggata ccaacaccat
     5401 actcgatctc atcttctcct agttcgaatc ctggtttccc tacttctggt acagttgctg
     5461 ctcgaagcgc tagaccgatc gtcttagttg cctgtacgac cttttctccc agttctttca
     5521 actcagctag ggaagcaccg tttcttgctg catcgcccac gattttgtgt acaaggacag
     5581 tacctgcaac tccgcgtttt ccagcagtat acgtactatt ttcaacggcg atatcatcat
     5641 ctacgatcac actttctact tcaatatcgt ccatagcagc taattccttt gccatatcga
     5701 agttcaatgc atcccctgta taattcttaa cgatcagcaa aactcctttt ccactatccg
     5761 cttccttaat agccgtctga atctgatctg gagttggaga agtaaacaca tcaccaagca
     5821 ccgctgcact taacatccct tcccctacaa acccggcatg ggatggttca tgtccgcttc
     5881 ctccaccaga aaccaaacca acttgttccg ctttctgatt cttggctaca acacgggaag
     5941 attctacacg atgaacaagt tctggatagc tttttactaa tccttccagc atttcttcga
     6001 caatattgct tggatcattc atgatttttt tcaatttatt cccatccttt ctttccggca
     6061 ttttgtgctt ctgttaagat ttgctgagta tcatctgtta taccggcagt gatggctgct
     6121 gcaaaagctc cttccactaa aggcgcatcc accagatgat aacgttgctg ctgctcttct
     6181 tccaacatgt cttttgccag ttccgagttc aataccgcgc taccgatatc cgcaaaaaca
     6241 aaataactag ctgcatcgct tgattcattg actgcttcta cgattttcat cgggtctgat
     6301 ccgatctcgc catttattcc gccgagagaa aaaatttgca cctcttcgga tttttgcatc
     6361 tgctcaatca tttctttaat accatctgtt actttttgac tatgagaaat aagcaaaata
     6421 ctttttttca caagaatctt cctttctttt acatagttgc taacgattgg tttcgctttc
     6481 attaatgaac aattcaagta tataataaat ttctaatttt gggaagatat gtgtagtgct
     6541 tcttgaagtt tgttatactt taattaaaag agaggagtga tcgaatgatt acaacaacaa
     6601 cgaacagtgt agagggaaaa gccgtagtag cttataaagg gatcgttttt ggtgaggtca
     6661 ttactgggat ccatgcattt aaagacttgg aggcggggtt gcgaaatatc tttggcggcc
     6721 gctcaaaaag ctatgaaaac gaactaatgg gtgcaagaga agaagcactt gccgaaatgg
     6781 ccgaacgtgc aaaagcatta ggagctgatg cgatcattgg cgtaaaaatg gactatgaag
     6841 tattaggcag cgacaatggc atgctgatgg tcacatgtag tggaacagct gtctccacga
     6901 gataagtagg ggacatctgt tttaataact aaagagagat gggacatgta gtggcactca
     6961 agaattagat ttttcagtct aacttcgagg tactctacct tcccatcctc tcttttttat
     7021 ttctttttgc ggatgattcc gtaagctgct gcgactaaca cacccgcccc tgctaaggaa
     7081 taactgacag aagctgtttt ttcatttgtt tttggcaaag tctgacttgt tgatttggat
     7141 ggctctgtcg tttgctgaac tgtttctgtc gtgcttccag aggtcactga tttttctcct
     7201 gccgaatgca aaatatcttt tattcgttgg aaactagctg ctaagtcagc tttttttgct
     7261 tcaaaatcat tagcaactgt ttgtgcttcg ctgatctttc cgtggattgc ttgactggcc
     7321 gctgtgatat ccgcttgagt ttgacttagc tttccagaag ctgtttgagc aacctgattt
     7381 aattgctgat tgaattcttt tgcttgttgg atcttcccgt caagatctgt ttggacagct
     7441 tccgcttttg ctttcgcttc ggcaagcttt ttctcaagtt gtgcacggtc atttcccact
     7501 ttatttttaa gatcagctaa atcttgatcg attgctgctt ggtcttcttt caatttgttt
     7561 tttgcagctt ctaatgcttc ttcgagttct tttcttttat tttccaagtc ttgcaaatga
     7621 tcgatttggt cagaagaggt agaactattt attgatgtat cttctgaaga tgaaatcgtt
     7681 tctcctgaat tatctgtcgg tacattggaa gtcatttctg tttccgaggc accgttttca
     7741 gcaccagaag agcttatagt tgttgaaggt tctgtttgag caggttcaga agtttccgca
     7801 aaagctgtct gtcccaataa gaaaatactg gaaacagcaa ttggccagat taacggcttc
     7861 attctttttt tcatcacttt tcccatcctt actacttttt aactaatttc ttattataag
     7921 caaaaatgtt tggaagaacc ataaacacaa caggctgttt gtctaaaaaa cggtgcatct
     7981 tttatttttt tgtacgttcc gggcttttcc atgtcccgct tccataacct aaaatcgttt
     8041 tgactgctgg aatgaaagtc gtcacgatcg ttatcgcgtt cactatccag aacaaaagca
     8101 tatatagtgg tgcgaaaaga aggtatttca attttcttcg tcgatcatct acaatcagcg
     8161 aagctagcaa ctggaaaaat cctgcgacca tttcaaagca gatgaacaca aaactgaatg
     8221 taaaagtaat ataaatctgt tcgtagtttc cttgatatac ataatgcccg attaatccag
     8281 caaaaagaac tacggaaata caaaagaata ttgaccagac aatactcaat gtttgatcaa
     8341 tgaagatgat cgttcttcca atatgtttaa aaggatggag catcactttt ttgaaattcg
     8401 tcagccagac ttctgttcca ccttttgccc aacgttttct ttgtcgatat agcatcttca
     8461 acgtcacagg tacttccatg aaaaagataa tctcaggagc aaaaacagat aaccagtcat
     8521 ttaactgatg atcccaagca atactgatgt cttctgtcgc tcgatcttgt ctgaatccgc
     8581 ccacatcgat caaggcttct tttcgataca tcgtatttgc tccactgtac gcataaagac
     8641 caccgaaaac agctgactgt gttcgtttaa tgatgcccac gatacttgag aattcaaccg
     8701 tttgagattt tgcaatcaat ttggtacgat tttggacatc catatttgca gtgaccgcag
     8761 caatatgtct tgcaccaggg cgaatgaaat aattcacata cctaatcagt gcatctggct
     8821 ccggtaccgt gtctgcatca ttgcttaaaa tcaatttccc ttttgcaaag gcaagtccga
     8881 tattaaacgc gtgggctttt cctttatttt tttcgatccg aaccacacga agattagcgt
     8941 attttttcat cagtctagct aatatttctg gtgtttggtc cgttgaacca tcgtctgtca
     9001 ctaatacctc atagttgtga taattcaatt tcgtcatcaa atactcgatt gtatcttcga
     9061 tcacaatttc ttcattatgg gctggcacca tgatcgtgat gaacggttcg acactcaagg
     9121 gaacatctac ccattctttt tgcttatgtt tgtataaaaa cacataacac cagacaccaa
     9181 taaaccatac aaatccgcct aaaatcggat aagaaacgag aaaaaataca taatagacaa
     9241 acaagtatct aagaataaat tacctgtatg ggtgattgag atcatttcag ttccacccca
     9301 ttttctttat acaaatctgc aatgaatgtc gtatctagat ttttctcttc ggagacagaa
     9361 taaaaacgga ccgtttctct ctcttctttt gttccaaaac gttccgtata cacttcttca
     9421 agtaattctt gtcttttttt caatttttca tcttcatatt caaaagattt atttactttt
     9481 ttgtatagat ttcgattgtt ccacctagtc aatacaaatg aggtaatcag gaaactgaaa
     9541 aaaatcaata caaaaaagcc gattaagaaa tacagcattt tcttttctga taaaaaagcg
     9601 tacaaggaaa aatccttggc tctttctggt ctcgttaatg ggaaaaagac ccatataaaa
     9661 ggaagaacaa cgatcatcca tcctaaaaga gccactaacg tttgccaaat ctttaaccag
     9721 taattacctt ttgaaaaata cttatctttc accaaaattg atgcaacttc ttctcttgtt
     9781 acttttttca tatcctcacc tcacgttttt ttgttacgac gtaggagttt accatacatt
     9841 tcatttcttt tcacataaag tgttcctaaa tttttcctaa gaaaaagaac aaaacatttc
     9901 tcatcagcaa atgaaaaata ttttgttctt tttatcagtt gttcttttta ttttttatct
     9961 cttaattatg ttgctctttc caatacgaca atgcttttca ttcctctaga tatcgccgtg
    10021 tacatttgtc tgcgtcttct ttgctcatct ccatcgtttt caaatggata taagattaca
    10081 tgatcaaatt ccagtccttt tgctagactc aaaggaatga tttggcagtt ttctaatcca
    10141 tatgaatttt ttttaatcga ttcttgtagc gtttgggttt cctcttctgt ttgggtgata
    10201 actgccgctt tttctccttt tagagaaacc aaaatctcag ctagtatttt caacaattct
    10261 tcagaggatc gaaaagagcg gtattcgggt tcttttcctt gcggtcggat cgatacgatg
    10321 tcgatcttct cttgatccac agcataggtt ttgaaaagct ctgtgattgc tccgctagaa
    10381 cggtaactat ttctaagatc ataccgatgg atcggcagat ggtagtcgtc gaaacaacgc
    10441 ataatatcag caaaagtagt ggaagaatta aagattgcct gattctcgtc acctactaaa
    10501 gtaaagtgag tttttggaaa taattcgatc aatagaccta attgtgccag ggtataatct
    10561 tgtacttcat caatcaatag atagcgaaat gctggtacat ttaccttttc aacaagtaaa
    10621 tgacgaatag ctaatataat gactgcttca tcaacagtac gtgtgggttg ccaagcataa
    10681 gctgagccat atcgtttttc ataaattctt tcaaacaact gatgctcatc cacccatgcc
    10741 atttcttcga tttgtctggt tatttttcga tatttttttt gtaaaagttt ccttgcataa
    10801 gctacaatac tttgttccga atcatcagaa atcaatttac cgaataattc caactgccta
    10861 tcttctgtca acgagagtac ctgattctgc acagcaggac tttttgcttg tttcaaaaga
    10921 tgattcttcc attcgctcat cagtgccttt ttcgttgctt gaagcctatc gatcaattta
    10981 ggatgagcag gaactttctc atatattttt tcgatcgttt ttttagaaag gactgttttt
    11041 ccatttttag taagtccacg gataaaattc ggatgatcta aaaacaaagg atctgattgt
    11101 tttagattat cgataaactt tttgctacgc aaacgttctg tctgctccga tacattttct
    11161 cccgtgatac gtttgaaata agcctcttca ccttctattt cctctgctga aagctgtgaa
    11221 accagctgta tgatggttag attcagtgga tttctttcac ctaatgatgg taggacattc
    11281 gaaatatagt cgataaaccg atgattaggt gatagaatca gcaagtcatc ggatgtcatc
    11341 gtatctcgat ataaatagag cagatacgca atacgctgca tgatagcaga ggttttacca
    11401 cttcctgcta cgccatttac caagatattt tgacttttca catcccgaat gattgtgttt
    11461 tgttccgatt ggatcgtagc agtgatagcc ttcatttcat tggtctcatc ttggcctaag
    11521 acttctaata atacgtcatc ttgaatagct acagacgtat caaaaaactt gaccaaacga
    11581 tctctttcca aaagaaattg tcgtctttct ttaataaccg tatcgatttt attttgacga
    11641 accgtataag aagatggacc aatcatgtta ttgtaaaaaa gttcggcaat tggtgaacgc
    11701 caatcataaa caagattttc atctgcatca tctgtgaaat ttgctgtacc gatataaaag
    11761 gattcttttg gctcattatc cagaaagtct acaacgactt ttccaaagta gggacttttc
    11821 aataaacgtt cgatctttgc tagctgattt tctgcgtttt tctgtttgat attcatctga
    11881 tcgatttctc tatttttcat ttcaatcatc gaaaaagtat ccaggttatc taagtaactg
    11941 tcaaaattca atcgaagctc gccagccatt tctttcaacg actggtttcc ttctattttt
    12001 gccgtatcca tcgattcccg cagttcgttt tttttatcaa ttagcttttg atacacgttc
    12061 ttcagatatg cttcttctaa ctgtttttgt gtgttgacca tacacattct cctctttttt
    12121 taaagtggtg gacccgatta gtttacctct tttaagagtc gaatgcaagc tttttaacga
    12181 gtatcatttg ttaggatata tcagttcttc aggtcggaca gtttgatata aagaaaatgc
    12241 accatcaagg ttttttacgt ggtagccaga ctgcttcaag attctttccg ctagatagct
    12301 acgtaatccg ctatgacagc tgacgatata ttcttgttgg ctgtctaatt catttaatcg
    12361 gtcccgaagt tcattcaaag ggatatgttt ggcttccttg aatttgccct tttccaattc
    12421 ttctgctgtt cgcacatcta ataaaatttt tccttttgct agttctgtcg gcaattcatg
    12481 ccattgtacg gtttcactaa gcccttctgc aatgttcatt gctgcatatc ctagcatgtt
    12541 tactggatct ttggctgagc caaatggcgg tgcatacgtc aattctaatt ccggtaaatc
    12601 gaagatcgtc aaatgccctt tgattgctgt tgctaaaata tcgatccgtt tatccacacc
    12661 ttttgcccca acaccttgtg caccataaat ctcgccagtc tctggatgga agatcaactt
    12721 cagtaaaata tctgttgctt ctggataata gcttgcatga tcttttcctg atacatgaat
    12781 gacttcaaaa gacagttcgt tttctttggc tgttctttca cttaaaccgg tggatgcggc
    12841 agtcattcca aacgttcgta taattgctgt tccaataccg ccttgatttc ttcgagaaat
    12901 tccggaaatc gtgtccgcaa cttgtctacc ttgacgattg gctggtgaag caagagaaat
    12961 aagtgcatct tttccagtga tttgctgttt tacaacgata gcatccccca ccgcataaat
    13021 atcaggaata ttcgtttgat aacgttcgtc tactaggata cccccgcgta tgcctaattc
    13081 gattccagcg gtcactgcta gctggttatc aggacgaaca ccaatggata aaataactaa
    13141 atcagcaggg atggtacgcc catcatccag tcgcaatcgt ttcccgtggt cttcaaatcc
    13201 agcgacagca tgagaagtaa tcacttctac tccttgatga gacaattctt tttctataaa
    13261 agctgccatt tcttcatcca gtgttggaag aatatgtggt gcttgttcga tcaccatgac
    13321 ttctaaacct cttcttttta ggttttctgc catttccaat ccgatgaatc ctgcaccaac
    13381 gatcacggca cgctttggct gtttttctaa tgcatgcatc actttatcga tatctggtac
    13441 atttctaata gaaaaaacat tgtcggcttc tgttatccct ggaatcgatg gaacaactgg
    13501 ttttgcacca ggagataaaa ccaatgcatc gtactgttca atgtgttttt gaccctcatg
    13561 tacgacttct accgttttgt tttcgggaaa gatggctgtc acttcatgat ttggacgcac
    13621 atctaaacaa aaacgagcgg ataatgattc tggcgtttgg acaagaagat tttcccgttc
    13681 actgatttct cccgaaagat aataaggcaa gccgcaattt gcaaaagaaa catacggtcc
    13741 tttttcaaaa acaacgattt ctgcatcttc cattaatcgg cgaagccgtg tagcagcaga
    13801 cattccacct gctacacctc cgacaataac gattttcatc ttatttccct cctactgttc
    13861 gatcaaacca ttgattcatg ccaccacgta cattcactac atcataacca gatttcttta
    13921 attctttagc tgcttgttta ctcctcatcc ctgattgaca aatcacatag acggtctttt
    13981 ctttgtctcc atcatatctg tttatacttt gtaatggaac atttttagct ccttttatat
    14041 gcccgccccg gtattctgca ggtgttctaa catcaagtaa cgctggattt tccaaaagct
    14101 tttcctttaa ttctgggact gttatcgatt ttattgattg aaatagcaaa ttcattttct
    14161 gcacctcatt tacattttta taaatacctc atggggtata ttaaacagga gctttattaa
    14221 tatgtcaacc agaatgactc ttcatttttt tcactacttt ttatatttgc ataaaatacc
    14281 gataaggggt attatctaat tataaaaata gaggagaatg tgttatggag attgataata
    14341 aaaatatcct gaaccgtttg aaacgtacag agggacaaat tcgtggtatc caaaaaatga
    14401 tcgaagacga aaaagaatgt atggatgtca tcactcaatt aagcgctgtt cgttctagta
    14461 ttgatcgcgt aatgggcatg atcgttgcag ataatctgaa aaattgcttt gagaaacctg
    14521 atagtaatcc agaagaacaa gcggctaaac tagaacaagc gatcaagatg attattaaaa
    14581 agtaaaggaa tgatttcaat gaaagtcgtt ttccatattg atgaattaga aaaatggtca
    14641 gaaacaggga aaaacgtaaa aaacctgata aaagcttctc cagaaacgac gattgtcgta
    14701 tctgtaaatg gcatcgcgat taccggttat ctagactctg ccaatgcaga attcttagat
    14761 ttgcaaggag tcgtttttca cgcttgcgcc aatgctatgc gtgctaatca tatttcagaa
    14821 agttctttac ctgaacaagt aatcgttgta ccagcaggtg tccttgattt agttgaactg
    14881 caatcccaag gttatgctta tatcaagcct tagtcagtag aggttgcgac agaagtgatc
    14941 agcttcaaga aatttcggct tatttttcga agaagctact tctggaaaac cgtgaatcaa
    15001 aggttgtgaa aacgctgtcc tgcatcaagt aataagaaca gccaaacaaa aatagcttct
    15061 catatttttc gtttggttgg gcttattacc gcagatgcaa gcttttgaac accgtttaag
    15121 gaagaggttg tgaaatcagc ggtttgacct aagtattaga gaaaaaaacg ggaaaatagc
    15181 tcctcatatt ttgccgtttt ttgagctaat accgaaggtc agctgattga acaccgttta
    15241 aggaagagat tgtgaaaaag tcgcttagtt tcgagtatta agaaaaattt tgtgaaaatg
    15301 gatttctaca tttttgcata aatttggctt aataccgaga aactggcttt tgaataccgt
    15361 ttaaggaaga ggttgtaaaa tcagcggttt gacctgagta ttagagaaac aaacggaaaa
    15421 atagctgctc atattttgcc gttttttgag ctaataccga aggtcagctg attgaacacc
    15481 gtttaccaag aggttgtgag cctaccattt aactccgaat cacaagaaat aatttcaaaa
    15541 acaacttctc ctgtttttgg tttggttggc ttattatcga gaagtagcta ctgggagtct
    15601 atttatcaaa aaaaggaatg cgacataggt cacacccctt ttttattttc tttgaaacat
    15661 ctttcggtac tcgacagcat acaagacaaa agattgtaaa agaccgtaag tgatgacggc
    15721 aaaaatgact ctcacccaca gcgttggtaa ttctgggaaa agcagcaaaa ggatcatgcc
    15781 aacatagatg acagcagtcg ctactttgcc ataccacttc gctccactta atttgatatt
    15841 tttagatagc ccgattaatc cagctacaaa cataaagcct tcttttatca agaatagacc
    15901 aaacaacagc caaagccacg gtcgatacca tgccatagac aaaatcagaa aaagttgtgt
    15961 caatttatct gcaaacggat caagcacctt ccctaagtct gttacctgat tatatctccg
    16021 agcaatgacc ccgtctagat aatcagacaa acctgagaca acaatgaccg ctcctgctat
    16081 ataaaatgac tgtctcagca caaaataaag ataaatcggc agcaaaaaaa tacggaatat
    16141 cgataaaaga ttcggaatcg tttgtatttc tttacgccag tctgtgctta atttcatttt
    16201 atcgccctac ctcctcagca ctcttttctt tttattttag cttacacggg gaataaaacc
    16261 aatgatttca ctgtttttat ttttctttac agccactgat attatgagct aatttgagat
    16321 aatcacttgc tgttttcatc gtttactctt ctttcttcct gtcgtttgcg catttctttt
    16381 actagtactt tatgagcttt ttttgatcct cgctctaggt cacgctgccg tttacgtgtt
    16441 ttataatcga taaacagact gtctaaatcg taacctgtcg ggtacagatc ttctgctgaa
    16501 cgcaccagac gaacacgctg acgaggcacg tggattattt ttttgtctaa atatacatca
    16561 atcgtatctt cacctatatc ttcataaact aacgcttctt tttggtattc attgacaaat
    16621 atccgatcac cttttgcaaa gagctggcgt tcttttttat tctccttcct atttgtaact
    16681 gctgtcttag gaagaaattc ttttttctgt gtccgatagg ctttttccgc caagtaacgc
    16741 tctgcttgat ggatcaattt catcgacata tccattttat gagcaatcca caaagcttgg
    16801 ctttctcctg tttctccaac ccgcaactga tatttaggac gaagtgcttc ccgatcgaaa
    16861 gccatagcag ccggcacaaa atcttcatga tctccggcga atttcttgat ttctccataa
    16921 tgggtcgtgg caatgatcaa tgccccttgc tcatacatag attccatgat tgcgatcgct
    16981 agtgcagccc cttcatttgg ctctgttcca ctgccaatct catctaaaag taccaacgta
    17041 ttccttttta tctttttcaa aatagcagcg atgttttgca tatgaccaga aaatgtactc
    17101 aacgcatttt ctaaattttg gtggtcaccg acatcgacga aaatctcatc aaagacagca
    17161 agctctgtcc cttcttttgc cggaacttgc ataccgaaca tcgccatcaa agtcaataaa
    17221 ccgactgtct tcaacacgac tgttttgcct ccggcatttg ctccggtgat aatcaagcct
    17281 cgatagtctt taccgatttc aacatccaat ggtaccgcat gctcaggtaa aaatggatgt
    17341 ctgccttgct tgataatgat atgttccgat ttattgattc taggtgtgct tccattgatt
    17401 tctcggctaa attttgcccg agcaaagatg atatctaaca ccgtgatcgt ttcgatcaat
    17461 aaatcgatca cttcttcgtt ttctgcgata gcaccagtca atgccgccag gacttgatat
    17521 tcttcactga tttcttcagc ttttaacaac tgatagtgtt cattcaattt agaaatcaca
    17581 gttggttcaa taaaaaccgt cgttctttta tttgattcat cgataatcgt tccatctatt
    17641 ttatttttat agctggcttt gataggaatc gtatagtatt cgcctttttg aacaatcatt
    17701 gcttcttgga tcatttcttt gtttttcgga tgacgcaaga atttcagtaa ttttgactgg
    17761 atttcttttt cgattagttg taattgtttg cgtactttac gcaaattacg tgacgcgtcg
    17821 tcactgactt tattattttt tattttctgg tcaatcaact cttctacatt tatcaaatca
    17881 ggaagatgtt tgctataagc aaacaacaag ggtgtctggt attgattctt atcaaaaaac
    17941 tttgtgatca tccgactgct tctaagaaaa tccgcatatt cgatcaaatc ggttggctgc
    18001 agaatcaacc cttttttgac ttgttctgtc agtgcatcga tcctcggcaa ccccataaat
    18061 ggaacatgct gactgctttc taagatcaag cgtgcttctc ttgtttcttc ttgacgatcc
    18121 aagacagttt gtaggtttgt cgagaccgtc atatcgctga ttcgtttttt gctatagttt
    18181 ccaattgctc ttgcttggat ttcctttttg atccgatcaa attgtaattg actaatcatt
    18241 tcgttgtcca tattctctcc ttttcttgaa gagatacctg tcccaaaata aataagggcc
    18301 tagagataaa taaaaggata gaacacagct taaaaaagtc atgtcctatc cttattagaa
    18361 tacaaagata ttgttttcct gacaggtacc gtttcataag cacaccaaat aaactttgtt
    18421 tattggctat tcgacttact gattagatac cagctacact cacaaaacat accccattct
    18481 tctaggtctt atttattttc cacttaacta tagcaagaca cattttggtt gtcaaacttt
    18541 agtatgtttt atagatttac accattaatg aatagacacc taccgcgata attgctccga
    18601 tcattggacc tgcgattggt acccatgaat atgcccaatc tgaagttcct ttattagcta
    18661 ttggcaatac cgcatgtgct aatcgaggac caaagtcccg agcagggttg attgcatatc
    18721 ctgttggccc tcctaatgaa agaccgatgg ctaagatcaa tccacctaca gcgaatacgt
    18781 tcgttccagg tgcaaattcg ttttgtccaa aagctagcaa cccaagaact aatacaaatg
    18841 taccaattaa ttctgtgatc acatttgctg gataattccg tatagcagga cctgttgcaa
    18901 acgttcctaa aatcgcgctt tcatctttcg ttgcattcca atgtggcaaa taagaaagcc
    18961 aaacaagaat ggcccctgca aaagctccta aaacttgcgc aatgatgaaa ggaacgacaa
    19021 gattccaact aattgcccct gtcatcgcca tagctagaga gactgcagga ttcaaatgag
    19081 ctgggcccat gaatccagaa atatagacag ccattgttac agcgagtccc caaccaaatg
    19141 cgatgacaat ccagccagaa gcctgtgctt tacttttgtt taaattcaca gccgcacaca
    19201 caccatctcc aagcaaaact aggatcagtg tcccaataaa ctctcccatg atttgagtca
    19261 tatcactgtt catttatttc tcccccttta gttcccgcag atctgattct gcaatcaatg
    19321 cttctaaacg tttagtctgt tgcgccttct cctcttctgt ccattcaaaa tatccagcaa
    19381 ttgcatcgat cacaggttgc ttgatctcat caagctgatc tcgttcaaac aacagatgat
    19441 ttgtacgacg aatgagataa tcgcctgggg ccaaaaccat ttcttcttct aaaccataac
    19501 gtaaccgagc tgactcagcg agactcaaac caggataagg tgccatttct tttgctaatt
    19561 caaagatacg tcgggcatta gtcccgtaaa aatcagcgat ataagtagca tcttcttcct
    19621 ctaaaccggc tgctactcct tcttttgcta attctgtcac tgtttcttct aatttagttg
    19681 gatcgaagtt tccacctgaa atctgatatt ttttagagtc gatttctttc gtctctattc
    19741 cgtattcttc ttttaataat tggcgtatca atcgcaaagc tccttctgcc atcttacggt
    19801 aatcggtgat tttcccacca gataaagtca gcaaaccatc tggttctctt tctaaagaac
    19861 tacctctgga aatcgtagaa ggtgcttttt catcacggct gttttccaaa tggttcaaca
    19921 cgtcttctac ttcagcacga gaaactttgt tttccttata ttcacttact gtatcgacaa
    19981 ctttattgaa gctcttgtct gaaatcgatc cattatctcc accattgtaa tcagaaccag
    20041 aattaccaat caacagtgga cgaagtcctg cccagcttgc ttcgatatca ttcaatgtga
    20101 tatttgcttc tggatagcga tgattgatca catccaatag ataatctaca tcttcttgtg
    20161 tgactttcgg atccgtaaaa tctccttggt aatccgtatc tgtcgtacca aagtaagtct
    20221 tgttttctct tggaatagca aaaaccatcc gcccatcttg ctttcctgta tcgaagtatg
    20281 tgggttgcgg tacaggcagt tttttcgcat ctacaactaa atggatccct ttagttggac
    20341 gcattttagg agagactgga cgcgtaaaat tcaagtttcg tactttatct acccaaggac
    20401 cactcgtatt gatcactaat tttgctttga tttcgatcac ttcatctgtc agcaggtcac
    20461 gagctttgac gccaacgatt tgatcgccat catataaaaa gccaaccgct ttcattttac
    20521 ttactagata agccccatct tcggcagcct ttttgatatt atcaatcact aaacgggcat
    20581 cattgttgcg gaaatctaga tagacacccg cgccttttag cccttctttt ttcaaaaacg
    20641 gttctctttc caatacttct tctggtgtca gcgtataatt ctcatatttg gttcctgtca
    20701 catttgccag tttgtcataa aggtccattg ctactttgac cgagaacata ttgaaggttg
    20761 ttgctccttc gccctcatag attggcaaaa gcattggatc tggttttggg atgtgtgggg
    20821 caatactttg tacgactgca cgttcaccaa ctgtgtcagc tactacttcc acatcaaatg
    20881 tcttcagata acgaataccg ccatgaacaa gtttggtcga gcgagaggaa gtcccttctg
    20941 caaaatcctg catttcgatc aatcctgttt tgattcctga tgctgctgac tggactgcca
    21001 caccggcacc agtgattccg ccaccgatga tcaacagatc caattcttct tgctgcattt
    21061 tttgaatgct atcttgtctt gtcttgtttg aaaacatatg gatttcctcc ttattcaaat
    21121 gcttgggtag cttttactgc ttttttccaa ccgttgtaaa gtttttctcg gcgctcttcc
    21181 tccatttgaa cttcaaatag cttgccagct tcataaaatt cgcggatctc gtctgtatct
    21241 ttccaataac cgacagctaa tcctgctaaa aatgctgccc ctagcgccgt tgtttccaag
    21301 ttttctgctc gttggatcgg tacattcaag atatctgttt ggaattgcat cagatattca
    21361 ttgtttgctg caccgccatc tactttcaat acaggaatct tgattccagt gtcatcttgc
    21421 atcgtatcga tgatatctct cacttgataa gcaatcgact gtaaagtagc tttaatgata
    21481 tcttcttttg tcgtaccacg tgtcaagcca aacatagccc ctcgtgctgc ctgatcccaa
    21541 taaggagcac ctagcccaac gaatgcagga acaacataaa cctcatcttc gcttgtagag
    21601 cgtttcgctg cgtcttctga atcgctagct ttttgcaaca tttgaagtcc atctctcagc
    21661 cattgaatcg aagaaccagc cacaaaaata cttccttcta aagcatagta aacctttcca
    21721 tttatgccgt aaccaattgt cgtcaataaa ttatttttgg ataattgcgg tttttcaccg
    21781 gtattcatga caataaatga acctgtacca taagtattct tgaccatgcc tggttcgaat
    21841 gccatttgtc cgaataatgc ggcttgctgg tctcctgcca tccctgaaat tggtgcttcg
    21901 cttccataaa aatgataacc ttgcgtataa ccataaactt ctgagttaga agtgactttt
    21961 ggtaacatca ctcgaggaat atttagtaaa tctaaaatct cctgatccca atctagctga
    22021 tggatattga acaacatcgt ccgactggca ttcgagtaat cagtaacatg tgcttgtcca
    22081 tcggtcagtt tccatactaa ccaagaatcg atcgttccaa acatcaattc gcctttttca
    22141 gctcgttctt gcgcgccttc tacatgatca agaatccagc gtatcttcgt tgctgagaaa
    22201 taggcatcga tcactaaccc tgttttttcg tggaaaaatt cttgatagcc ttcatttttt
    22261 aagttatctg caattccagc tgactgtctg gattgccaaa caactgcatg atagattgga
    22321 cgtcctgtat ctttttccca tattactgtt gtttcacgct ggttcgttat gccaatccca
    22381 gcaatctgat ttggtttgat ccctgattcg atgaacgctc cggcaataac tgactgtaca
    22441 gaattccaga tttcatttgc atcatgttcc acccaacttt cttgcgggaa atattgcgtg
    22501 aactcttttt gagaactgcc gatgtgtctt cctttcttgt caaagataat tgctcgtgag
    22561 ctagttgtac cttggtcaat cgccatgata tacgttgatt ctgtcattgt gattccctcc
    22621 attatatgca aacgctttct ttaactactc ttatcatact actgttttaa aaaaagaaac
    22681 tgtcaaaatt ttattctttt tttctttttt tgacagtttt ttattatgaa gctatttttt
    22741 aatcaattct tttctttttt cagcgtctct attcttcttt tcaatcgaat aatgtcgtaa
    22801 ggtgacgcaa attctgacag ataatattca cactgttttt tgtttctctg gcaaaatgtg
    22861 atcaccaaat catattcttt accttcttca taacttccaa tctcaatacc gccgattccc
    22921 ctcagttctc tgatcagata ttgttgaaaa gcaatacgat aaatcggcag actttctaaa
    22981 tcgattccaa tcactactgg ttttgcaata taaaaatcga tcatcaaaag aacattggca
    23041 tactggatca tcaggtaact aagctcacta tcttcagctc ttttcctcgg caattgctgc
    23101 accgctgttt cctgtaattt cgccagtaac tgtgttaagg aatgcccgag cattttctgc
    23161 tgctgttcca aaagccgatc acgctcatag acttcgatct ttccaacaaa gaaaaaccat
    23221 tcattgttga tctgggaaat ttggtagcct actgcctttt cttctttgat tttcaaacga
    23281 cgaggacgat aatgccataa catcgtttcc cgaatgtaag tatctaaaac agcggttggt
    23341 aactttttgc tccgtgtcaa atcatactga taaaatattt cttcgtctac tatcgaaaaa
    23401 gaaacaaaaa agctgtaaaa catcattgtt tcgtatacat tcagttctgc tgctgttcga
    23461 ctaagatgca tcacgatgat tggatcgatt gcttgataaa gccggtccga ctgatagagt
    23521 aatttctttt ctttcaaagt tgcgtacgta cttttttcat tcaatagtct ttttttcgta
    23581 atcccgagcc agcaagcaat ctgtgcttct gctgatgccg taaacgttgt ttctaataca
    23641 cggtccaatc ctagaatgaa cggtcttttt tctggtgtat tttgcaaaaa aagcggtcgc
    23701 tgatcttccg ggatatattg gaatagttca taataaaaat atcgtatctg tagctcttct
    23761 ccgtataatt gaccgttttt gatttgtaat ccaaattcct ctaagagctg gttcaattct
    23821 ttgatctttc gaaaaacaga agactcactg atattaaacg ctgtcgctaa tcgaacgatg
    23881 gatacttgct gatgttctaa taaatagacg agcatcttaa acttcaagct ttcctgcaat
    23941 aaaaacaata gaattttttc caaacttaca tcttcgtcca acacaagcag cgcctgatac
    24001 tcatttcgct gtacttctac ttttttttcc atcattcggc caatcaattc aatatcttcc
    24061 aaatattgat caaacgaggc ttttgacagt tggacatgct ctctcaaacc attgatagag
    24121 gctgttcccc cttgcagaac aaggtttcgc aaaatcagga tctgtaactg ctcttttctt
    24181 tctaataatg cttcaatctt cataggcagt attcgccacc tttgaccaca ccatatctgg
    24241 cacacggtat tctggctgga aaaatagctc atgttcttct aactgttcaa acgtccatcc
    24301 ttcttctact gcaattgtca gttggtcaag aaggtggaac atctcttttt tgctttggag
    24361 ctgtgctcca agaatctttt tttcagaatc aaatagaaat ttgagtttaa ctttgctttg
    24421 atttattgct gtaagcttgt agtctacgta tcctgtttca attttttggg gataaaaaat
    24481 gctctcttct tctgtcaaac ctacgcttcc taagaaatag ccaaaccact gattgccgac
    24541 tggacgatag gttccctgat cctttgtctg tgagccacta attgtcttgc tgacatttcg
    24601 tgctgtacgg acagcgttat ttactagaga aacatacatt ttttcatttg ttggacgaaa
    24661 agttacttgg atcgcatcgc caatcgcata aacgtctttt tgagacgttt gcaagtagtc
    24721 atctacccag atcgttccat catcattcaa tgttaattgt tcttcccaca tctgattgtc
    24781 ggggcgagaa tgattagcca gcaaaacaag atcatagttc tcctttcttt tttccgtctc
    24841 tacaatagcg gtttcatttt cggtcaaaga cacaacttgt tcttctaaaa aaagtgaaag
    24901 cccttgattt ttcatttcct ttactaacgg ttctgtcatt tcaggatcta gataacgaaa
    24961 aagaattgac tttcggcttt cgtaaagatg gatttgtttt tttcttgatg ccaacccttc
    25021 agctatttcg attcctgctt gtcctgcacc aataattgca attttctttg cattcatgat
    25081 cttttgctga agagccgtta cgttttgtat ctttttaact gggtgaataa gcgttgattc
    25141 tgcagaaata tttcgaaact tttgatttga accagtcgct agaatcaaac gatcaaaatc
    25201 caattttttt cctcctgcta aaacgacttt tttttctttc aatcctatta ctgtcttttc
    25261 tgtgtaaaca tggattcgat agcggtcagt cagttcttct ttcgtgatcc aagaaagttg
    25321 tttttcactg gtaacctttc cttttaatat caaattgaac ccgctgggaa tccatcctaa
    25381 cttcgattgc ttttcaatca aatatatttc acttgttggc atcaattggc gaagataaat
    25441 agccgcatga atccctgcaa acgacccgcc gacaattaca atgttcatct gcattcctct
    25501 ttcttataag gtgttctact gctaacttct caatcagaaa acacagtatt cataactcgc
    25561 tttcaacctt atgatacata gcgatgcagc agaatcaacc ctctccatca taccgtgttc
    25621 taggtctgct tctcaataat gtactagtgg acctaattga agagtatcat ttctgaagag
    25681 atgtttcaaa aaatgtactc ttataaaaga aaaaaactcg ccttttacgt acctctgtcc
    25741 aaataggaaa cagaaaagaa cgaaaagacg agttttagcg tgtgtaatgt aatagttaga
    25801 atagacacat catttatttt taattcttta atgcttcaac aactgcttcg cctaattgat
    25861 gagcagattg aggattttgc cctgtaagaa gccgcccatc aatttgaacc acttctgaaa
    25921 aagctgctcc agactgatag tttgctcctt tttctttcaa agcatcttct gctaagaatg
    25981 gtacttcatt cgttgtacca tttgcttcct cttcttcatt actgaaccca gtcactgtac
    26041 gtccagctaa aatagatttt ccagactcat ctgtaactgg caacaatcct acaacaccgt
    26101 gacaaacagc tgagaccagc ccttcatttc gatagatttg tgaagcgact ttcccaagat
    26161 ctattgcttc tggaaaatcc cacattgttc cgtggccacc agcataataa accgctgcat
    26221 aatcatttgc atccacatca tcaggacgta aggtatttcc caaggcccga tttctgaaat
    26281 cttcatcttc ataataggcc caatcgattt catccatatt caaaacactt tgcggttcta
    26341 aaggtacata tccgccttta ggactgacaa aatctgcatc gatcccattt tttactaata
    26401 catcataaaa atgagtcagt tcacttaacc acaaacctgt aggacgctcg atcttttcgt
    26461 acttttctac atttgttaat acgatcaata ctttcatttt cccatcctct ttctataaaa
    26521 attgcgttat tgataacgtg ctttcaagaa attatataat gggcttggca aataaagctc
    26581 tttatatgct tcttcaaaag gtttcgttac aaattcatcc tcacggtttt ccaataattt
    26641 ctctgcccag tcaggatcga cgatcatttg ctgtccaatt gctgcaagtt ctgcatattt
    26701 caatacctgc tctacatctt cgcgattgcg gatcccaccg acaccaacta aagggatctt
    26761 tccttgaatc gtgttatgga ccacttcaag aatcgattta tcgttgtatt tatcagaacg
    26821 agctattcga tcatacatat ttaatgaaac atgcagataa tctagttttg actcacgtaa
    26881 tcgttcaaga agccagatcg tgtcttcaaa acggatacct ggtgtttcat attcttctgg
    26941 tgagaaacga taccctacga tgaatggttt tttagcatat tgttcaaccg ctgagaaaac
    27001 cgttgttaat aattcttcaa taaaacgata acgattttcc aagctaccgc cccattgatc
    27061 ctctcgtcta ttcgaatgcg gagagaaaaa ttgttggatc aaatacgtat ttgctccatg
    27121 caactcgatt ccatcaaatc ctgattcaat cgctctacgt gtggctttgc caaatgcttg
    27181 gattgtttca acgatttctt cttcactcat tgctcgtggt gtttccgcat caggacgttt
    27241 tgctgcaact gcacttgcag atacaggctg ttctccagat aaagttgctc gacttgtcat
    27301 tcgtccaccg tggaaaatct gaataatcgc ttttgcgcct tctccttgga ttgctttcgc
    27361 tagctcactt aatcgaggaa tcatttcatc atgagcgatg ctgagttcac ctggccagcc
    27421 ttttccgata tcttggacat ttgcagcacc agtaataaca gcaccaagac ctttagaacg
    27481 ttgtttataa taagtgatct catcttcggt gatcactcca ttgaaaaagc tgagttgtgt
    27541 cgtcataggc gacagcatca atcggttctt caattgaact cctgaaggta aagtaattga
    27601 ttgatcaaat tgtgacattg tcattcctcc cataattcgc tctgattaac ctatcgagaa
    27661 tcatctatca ttaaaccact cgattagttg tacatgcaac taatttgctt aaaaaatttt
    27721 attttttttg taacttatct gttgcatcat tcattgtctg ggctaattct ttacttgctt
    27781 catttcccaa aactgctttg tatgactgat gaagttcttc ccagctacca tcgacttcct
    27841 tagcaaattg gcgtccttgt tctgtaagat aaactaatga caagcgacct tcgctaactg
    27901 ttttgacgta tcctttatat tctaattttt caacaaaacg agcaatcgtt gatggggcgg
    27961 aatagagcat ttgtgcaata tctttttgtg gtattccatc tttttctttt aatatcaata
    28021 aaataaaacc ataagttggt gccagacctg tccttctaaa cgcttcatca gctaatttat
    28081 tcaacatgcg atctagcttt ttcacgctaa agaaaagaca ttcatctaac gctttttcaa
    28141 ttggcttttc catttcatcc cctccattgg cgtacatatc atagtatgat ttttattttt
    28201 tgtcaaaaaa cacggaataa tgtccgcctc ctctgtctag atttgaaaaa aagaagtagc
    28261 catcttcaaa gaatttcggc ttgttttcga caaaactcct tctggaacac cgtgaatcag
    28321 aggttgtgaa aaagtcgttt agttttgagt atgaagaaaa attttgtgaa aatggctttc
    28381 cacatttttg cataaatttg gcttaatacc gaaaaactgg cttttgaaca ccgtttatat
    28441 cagaggttgt gaaaaagccg tcctgcatca agtaataaga acagccaaac aaaaatagtt
    28501 cctcatattt tgtcgttttt tgagctaatg ccgatggtca gctgattgta caccgtttat
    28561 tgagaggtcg tgagcctgcc gttgaactcc gagtcacaag gagcaatttc aaaaaacagc
    28621 ttctcatgtt ttatgactat ggtgacttgt gatcgaggag ttggcaggag aatactgttt
    28681 attcgtaaag aaggggctgt gacaagactt ttgtcacagt cccttatttc cttttattat
    28741 aggatttcgc catttgattg gatgacttgt ctgtaccaat caaatgattt tttctttaac
    28801 cgtttctttg tgccattccc ttcgtcatct tgatcaacat agatatatcc ataacgtttt
    28861 gacatttgtg aagtagaagc gctgacgata tcaatgcagc cccacgacgt ataaccaaat
    28921 aaatctactc catctttgat agcttccttc atctgctcaa tatgctgttt caaatagtca
    28981 atgcggtaat cgtcttgaac ggttccatct tcttctactt tgtctatagc tccgagtccg
    29041 ttttcagcta tgaacagtgg tttttgatac ctatcatata attgattcaa agcaattctc
    29101 aaacccttag gatcaatctg ccagccccat tcacttgatg gaagatacgg atttttaatt
    29161 cctccagtta ttaagtttcc ttggacccct tcccaatttt ctggatgagc actgactgca
    29221 ctagacatgt agtagctaaa agaaacaaaa tcaacagggt aggctgctaa gatttcttca
    29281 tcacctactt ctatttttaa gttgacacct ttttcttcaa acattcgttg cgtatacgct
    29341 ggataatatc ctcttgcttg aacatcacta aagaacaaag aagttctttg ctgctcaatt
    29401 gttttttgaa tatcatccgg atcacaagaa tacggaacaa gaagctgata gctgatcata
    29461 caaccgactt gtgaaccttt gataatttca tgacaagctt ttgtagctaa agcacttgct
    29521 ataaactgat ggtgtgcacc ttggtatttc gcttgttcca aatggggatg gttttcttca
    29581 attactccta cgctaacaaa agggtgatgt tttacacagt tgatctcatt gaaagtcatc
    29641 caatatttta ctttattttt atagcgtttg aatactgttt cagcgaaacg aacgtaaaac
    29701 tgaataagct ttcgattagg ccatccacca tattctgtaa ccaaatataa cggcatttca
    29761 taatgggaaa gagaaacgac tggctcaatc ccatatttta gacattcgtc aaacacttga
    29821 tcataaaaaa ataatcccgc ttcattgggt gtttcttcat caccatgagg aaaaattctt
    29881 gtccatgcga tagacatccg atagcattta aaacccattt cagcaaataa acggatgtct
    29941 tctttaaagt gatgatagaa atcatttccg tgtcttttag gataataata cttgctgttg
    30001 ggatcctgcg ccgcttttac ttgctgatgc gtcatcatat gccattgatc tgtccatttt
    30061 tctttaggta aatcgctgtc gaatgtatac acatcagaca cagataatcc tttttcatct
    30121 acatcccatg caccttcaca ttgattggcc gctgtcgctc cgccccataa aaatccttct
    30181 ggaaattttt tcatatcgat ccccctaaac ttgctagttt tcttgtttga ttatttttct
    30241 tatgactagt aatgatattt ttgaatctac tactatttgt aacaataacc attgtggtcg
    30301 gatcatatcc ctctttgatt aattcttgca aatctacttc catcaataaa tcgcctttac
    30361 gtactttttt tccatcagat actgcacttt gaaagtattt gccatttaaa ttcactgtat
    30421 caataccgat atggatcagt atctctgctc cttcttcact gactacccca attgcatgtt
    30481 tcgttggaaa aactgttgaa acttccccgt ttacaggact gtagattttt ccgtccgttg
    30541 gaatgatcgc aaacccttct cctaaagatc cactagcaaa tgttggatcg tttacttcag
    30601 ataaatcaat tatttcacct gctgcaggag tacttaacac tatatccttt gaggattctt
    30661 cttcgatttc atccttgtag ccgaaaagaa aagttagcat acaggaactg atcatcgcta
    30721 agaaataact agcaactagc caaacaaatc cttcaccaat ataaatcggg aaagacaaaa
    30781 tacttcttgt agcaactgcc aaagcaccgg ctcctgcggc accagctact ccaccaaata
    30841 tagctccaca tatacaagca atgtaaaatg gtcgtttata tttaagagtc acaccataaa
    30901 cagcaggttc agtgattcca aataatgctg tgataaatgc agataaagat aaacttttta
    30961 ctttcttatt tttcgttttc aaaaataccc caagtactgc tcctgcttgc gcagctacgg
    31021 cagtcataca tgctggacct agcgtatcac gaccgtagaa agaaagattg ttggtcatta
    31081 ctggtacaag tccccaatgt aacccaaaaa tcactaacac ttgccaaaat ccgccgataa
    31141 cagcaccaga taaaacaggg ctaaagttat aaacagctaa atatccagct gcaatcagct
    31201 gacttgcata atctgcaatt ggacctacaa ctaataaagt aattgggacc atcacgatga
    31261 tacaaatgaa tggtgtcaat aaatttcgaa tactttcgtg caagcttttg cgaatcttag
    31321 gctcaatata agaaagaacc catacagcta aaattgctgg aataaccgta cttgtatacc
    31381 ttgctagaat cacgggcatc cctagaaatc gcaaggttat gctatcacta tatgcactga
    31441 caatcgtagg ataaacgaga gctgctgcaa taaccatagc gatgaaacga tctgtattga
    31501 acttttttgc agcagtatat gccaaaaata taggcaagaa atagaaaaat gcatcagctg
    31561 ctgcattcaa aatgatataa gttcctgatt cagtagttaa taaattcaaa ctcgtacata
    31621 aggcgagtac tcctttgatc aaaccacttc ctgccaagac gcctaaagtc ggggcaaaaa
    31681 cagcggcgat catgtctata aaagagttta aaatgccttt tttctcagtt cctctctttc
    31741 cactagatgc atcatcttct agtttcattt gtttggataa tacgtcatac acttcattta
    31801 ctgcatttcc tactacgact tgaaacattc ctccagcttc gactacagtg atcacacctt
    31861 ctagttgctc taccttctcc ttatctgcct tcttattatc ttttagggta aagcgtaacc
    31921 ttgttgcaca atggtaaaca ttgctgacat tttcttcgcc tccgacaaat cggacaatat
    31981 ctttcgctaa ttgtgaatag tccattttta cttccccctt gttaattaat gccgtagcaa
    32041 tccttcttac aagataagta tagttcttag aaaacggttt ctccattcaa ttagtacccc
    32101 ttataatagc gaacgaaact gtacaatagt gtgtatggtt tacttagccg agcaaatcac
    32161 tcactgcttg aaagaaaaca agtatatcgt ttctcttata gtcaaaggag acaaaaaaaa
    32221 cacgcttgat cttcctttgc tgatgtataa gtggtatctc aagctgatac tctgtgattt
    32281 ttgtttctac ttggcttttg ataagacagc acaacaaagt ctcggcattt aacggaagca
    32341 taactaggtc agaactagga gagattagtt gctgaatgga atctatcttt ttatggattt
    32401 ttatttgatt ttcttctttg tagtaatgat caagcagttt tgttttcttt ttttcttcaa
    32461 ctatggtcag cttttcacgc aaaatctgat aaatctccca agattcaaac tcatctagaa
    32521 cactaggtag ataaatcaaa tcatcccact caaaaataag ctcttgctca gtagtcaaaa
    32581 ttgcatcata ttccgttatt ttgtttgatt gatttttgaa aagatacact ggttctatcc
    32641 ggcattcttt gatccacatg ttcatacttt gttgaatctg tttttttagt gtattcaaaa
    32701 tactaattgg ctggttacta acaatcagta tctccccttg ttttcgaaaa ggattcaacg
    32761 cttcggcaag atacaaaaca aaataagcgc tttcagctaa attgggacgt aattgaaaat
    32821 gtattgggaa gaaacgccga attaaataaa tttctaaagg ataacgggag atgatctttt
    32881 gtacaaaatg attcatggta ttatgtccgg catccatcct cgttttcatt tgtttgatat
    32941 gggttagcaa aaattctgtt tcatggaaca ataacgaaac aggaagatct aaaaaacgaa
    33001 taatcgtttg ctcaaatgct cttagattct tctctatttc tttgtttttt tcatcattaa
    33061 tcatcaaata atttaactct aatagtcgat tttcaattgc ttgtttttct tgattggaaa
    33121 aagaataggg tagttctgat gataactttt ttgtgagggt ctctaccatc ggaataaata
    33181 atgtatacct gtttgttttt tctaaaagtt cttctcttcg aagaatggtc aaaacgagat
    33241 accgagaaaa tcgtatgaag tcctctcctg aaacaatata attatatgat ataagaacct
    33301 cttttaatgt cttttggaca gtcggcatga tattggaaaa aattgtactg atttgttgag
    33361 gcaaccttgc gtgttggatc accgtttctg ttccaacaag agataagaaa attctttttg
    33421 aattttcttc agcaatgatt tttagtcctt ttgtcgacga aacttccagt tctaatttag
    33481 gattgcgctc gatccaacgc aaaatcgttt ttatttgtaa tgatacggct gtttttgaaa
    33541 gaaagaaaat gtctgctatc ttttgcatac tgacataccc tttttgaaac aataagtacg
    33601 tcacaatagt tgaagctcgg aaatccatgc tggaatagat tttatgtttc gtcacctctt
    33661 gtatgatttg ctgtttagtt tcagattcaa ttgattctaa aaaataacct gaacgtttat
    33721 ttgactggat aatgattttc ttctcgtaga tagcaagcat atttttgatt tcagtttgga
    33781 tcgttttctt gttggtacct aaagcttcag ctaattcttc gcttgttatc caatgattct
    33841 tatttcttaa tactacaaga atttggagtt gtcgtttatt taagtccatt tgcttacacc
    33901 tcctctttgt tataaacata caggaaaaac ctctcaaatg acaatagtca cttgcagagg
    33961 tttaaaaatc atttgaagaa agatctatat tcaaggtttt taccattaga ctggataatt
    34021 tgtttataat attcaaaact atcttttttc aatctcctaa agcttccgct cccatcattt
    34081 tgtctatcca cataaataaa gccatatcgc ttcctcattt cccctgttcc tgccgatacg
    34141 atatcgattg gtccccacca taagtaacca atgatttggc aaccatcttt tattgcctca
    34201 gcaatttcct gaagatgtat ttttagatac tcacaacgtt ttggatcgtg gattcgattc
    34261 aattcatctg gtgcttcatc tagtccaatg ccattttcaa ctatgaataa tggtaattca
    34321 taacggtctg tcaattcatt catgacataa cgaaacccta gaggatcaat tggccaactc
    34381 caaggttcgg gagatttttc cttcaaaaag ggattatcga aaccatttgc tccgccagta
    34441 tctacgcgca tttctgcttc ttttttatag acagcgctac gataataact aaatgcaata
    34501 taatctgccg tgttagcttg caagcactgt tcgtctccat ctaacattac tggtgcacag
    34561 tcttcttcct tccatgtcct gtaaatataa gctggataat gccctcggca catgacatcg
    34621 gagaaaaacc atgaattgcg tttaaattgc agtgaaccaa atacattctc tggatcacag
    34681 tcatatgcat aagtcgctaa tgcagaaaag ctacacataa tacccatctt agcatttgga
    34741 tcgattttct ttaataattg agtagcacgg gcgttcgcta gaaacatatg atgacaggct
    34801 tgataaatca ctttttttgt tagtgtagca atgggttcaa gtttatctct agcagggaat
    34861 gtaaccaatc cagcagctgc ataaggcata cgaaaagcat tattgatctc attaaaggtc
    34921 atccaatatt tcactttccc ttggtagcgt tcaaagacgg tctgcgcata aaattcaaaa
    34981 aagtcgatta attttcgatt ttgccacccc ccatactctc ctaccaatgc taagggggtt
    35041 tcataatgac taagagtaat tactggctcg atatcatatc gatttaatgt attgattagt
    35101 ctgtcataaa atacgagacc agcctcattt ggcttctgtt cgtctcctct tggaaatatt
    35161 cgtgcccatg agatgcttgt tcgaaaagct tttaaaccta actccttcag taaacgaata
    35221 tcctcttcaa atcgatgata aaaatcaatc gcttcatgag aaagataatg gagtgattcg
    35281 tctaaagcaa tctcccaaat ctttttttct tcattccact gaatactagg atgctggttc
    35341 atgatcccga taagcgtatc tgtcacatta ggtaatttgc cgtcttccaa ccatgcacct
    35401 tcacattgat tagcagctac tgctccgccc cataaaaaat tttctggaaa tatttcctga
    35461 tccataacta tctctcctcg ctatcaacaa tttcttactt gaagtatagc tcgattcatc
    35521 aaaacaagat attctaacta tttcggcaga agagaagaaa aaaattgaat gctactcttc
    35581 atttttaata tttatatata acaaatacat aagacgatcc ccgaaagaaa acactcgtta
    35641 accatctaag caaacgattt attttacttc caacacaaat gcttcataag gtcgtaaatc
    35701 aactttgccg tctggtaact gtttcagagg ataatttgac acaataaccc ttaaagcttc
    35761 acgtggaaca gtgaacgtct gcttttcact ggacatatta caaataacga gccattgctg
    35821 gtcttcatat atgcgagaat aggcaaagac atgctcaggc gtcttttcca ctaattcata
    35881 gtctccccag acgatgatgt catgttttct acgcaaatcg atcaatttct tgtacatata
    35941 aaaaacagaa tcttgatctg ctaacgcttc ttctgcattg atttctttat aatttggatt
    36001 gactcgaagc catggaattc cagtagtaaa cccagcatgt tcacgggcgt tccattgcat
    36061 aggcgtccta gcattgtctc ttcctttcgc attgattgat tccaagattt cctcttttgt
    36121 aaatccagaa gaaatccttt cgttatacat attgattgtc tcaatatctt ttgcttccgc
    36181 gatatcatcg attgggcaat tcgtcatgcc taactcttct ccttgataga tataaggggt
    36241 gcctttcatc atatggagca agatagcaaa catcttggca ctttcttttc gatacacatc
    36301 gtcatttccc caacgagaaa caattcgcgg aagatcgtga ttattccaaa ataaactatt
    36361 ccatccttca tcacctaaag atgtttgcca tttggataat acttttttca gttcagagat
    36421 actcaagggt ttcaaatccc atttgtcttt gccttcttgc tgatctaatc ctatgtgttc
    36481 gaattggaag accatggata attcatttct tttaggattg gaataaagtt tagcgatttc
    36541 ggtagttgct ccccatgttt cgccaacagt catcagattt ttgtcaccaa aagttgcttg
    36601 attcatttcc tgaagatact catgcagttt aggtccatta cttgtgattt ctttatccgg
    36661 ttctttgcca atcatatcga tcacgtccat acgaaagccc ccaatacctt tatcgatcca
    36721 gaaattcatc atttgataaa tctcttggcg aacctgttta tttttccaat tcaaatcagg
    36781 ttgacgcttg ctatacaagt gcagaaaata ttgccccgtt ttttcatcca actgccaagc
    36841 agaaccactg aatattgagc gcaatctatt aggctctttt ccattgaccg gatcacgcca
    36901 aatataatag tcccgataag gattttcttt atttttacgg gcctcaatga accacggatg
    36961 ttcatcagaa gtatgattca ccaccagatc catgatgata cggatatttc ttttctttgc
    37021 ttcttcaatc agttcttcca tttcttccat cgtaccgaat tctgccatga ttgcttgata
    37081 attactgata tcatacccat tgtcatcatt cggagactcg tatactggag aaagccaaat
    37141 tgcatcaata cctaaatacg ccagataatc caacctggaa ataatccccc gcaaatctcc
    37201 aataccatct tgatttgaat cttgaaaact tcttggatag atttgataaa caactgaatg
    37261 gtgccaccat ttttgttcca tgattttcct cctataaatg gatgtttcta ttttttgctt
    37321 aatcatttat tttagtctct ctcactaaaa attgtaacgc tttcaaatag ctattgcacc
    37381 ttgttatcgg taacagaata aaacaaaaaa acagctgagg aaattccctc aactgttttt
    37441 tgaaaggatt gatttatgca gggaagaatg ctgatttcac aacttcttta taaacggtgt
    37501 tcactcagct gtcccttggt attagctcaa aaaacggcaa aatatgagga actatttttt
    37561 cgtttttttc tctaatactc aggccagaac actgatttca caacctcttt ataaacggtg
    37621 ttcactcagc tgtccctcgg tattagctca aaaaacggca aaatataaga aactattttt
    37681 tcgttttttt ctctaatact caggccagaa cgctgatttc acaacctctt tatttcacat
    37741 tcaatatcca catgaatccc acaaatacaa agaacagacc atacatgatt ggagatactt
    37801 cttttcctct tttagctgct aacatggtaa ttggatagaa gataaaacct aatgcaatcc
    37861 catctgaaat actataagtc aaaggcatgc cgatcaagat caagaatgaa gggatcgcaa
    37921 tctctaagtt tccccattcg atttgtcgca aagattgtgc catcaatact cctaccataa
    37981 tcaatgctgg tgcagttact tgtgaagtaa caactgatag taatggagaa aagaacaagc
    38041 ctaacaagaa gaaaatccct gtagtaatag ctgtgatacc tgaacgtcct ccaacggcga
    38101 taccagctga tgattctaca aatgcaccaa caggagaagt accaaataag gaacccgcaa
    38161 gcatagctgt tgaatcagaa gctaaagctt tccctacacg aggcatcttg ttgtctttca
    38221 tgaaacctgc ttggtttgct aatccaacta aagttcctgc tgtatcaaag aaagtcacaa
    38281 gcaagaatgt cagcactacg acccacattt gaagagaatt gatgtcccct acatgtttta
    38341 atgcaactaa aaatgttggt ttcaaacttg gtgctgcggc aacaatttca gaaggcattg
    38401 gaatcaaacc tgtgaccaga cctaaaatcg ttgtagctgc tatcccgatg aagatgccac
    38461 ctggtacacg ccgaacaagc aagatagcag ttacgactaa tccaaaaatc gtcaaccaag
    38521 tagaaccaac gttgagtgat cctaatccta ctaatgtaga ttcattcgaa acgatgatcc
    38581 caccttcgct cagaccaagg aaagcgataa atagaccgat accgccagaa atcgcatatt
    38641 tcaaatctgc tgggatcgca tcaatgatca tttcccgcag cttgaagatt gtgatcaaaa
    38701 taaaaatcaa agaagcaacg aatacgccgg ctaatgctgt ttcccatgga atccccattc
    38761 ctaaacaaac agaataagca aaaaaagcat tgatcccaag agcaggtgcc gttccaattg
    38821 gataacgggc taataccccc attaaaacac agccgatcgc actggctaat gcagtcgcag
    38881 taaacactgc tccttcatcc attccagaag cacctaatac agttggattt acaaataaga
    38941 tataagccat tgagataaat gttgtaaatc ctgccagtac ctcacgtttt accgtggtgt
    39001 tcaatttttc tatttcgaag taggaaagta ttttttcctt catcttccat ccccctaaat
    39061 ccgaacatta taaataaagt ccgttaaaat ttcgatttat taataaaaca acgtgtttta
    39121 tcatatacta aaacaaaaca taagtaaaca aaaaattcgg attttaagca aaatccgaac
    39181 gttccatctg attaaatccc tttatctcaa agttcatgaa cattcacttt ggattacaag
    39241 aatttcttta ttgtgttaat aaagtttctt cattctagtc tcaaaaatca atagtgtgat
    39301 ttgattttac catttcgttt ctgttcttct ttgatgaatt cttttgtatc gacaggattc
    39361 agattattta gtccgccaaa acctgtatcg accatttttc cccagccttt tgtcttcttt
    39421 tttcctgatt tgagattctt tgctcgaagc ttctcactat gttttgctcg ccgatacttc
    39481 aaataacctt ctaatcttcc cattagatcc actgtttttc ctccttttta ttcaatcaga
    39541 cgactcagct tttcctataa caaaataatt gccttcaggg tcagaaaaat tgaaagtcaa
    39601 gatgccattg ttatctgtga tttctcctgg ttcttccatg ttctcgtaga tagtttcaaa
    39661 ttcatccgta aaaaatacta aagaaggcgg tggtcctaat acctctggtg aaaacttctg
    39721 cacaaatact tttgggaata cgcctaattc taattctttt gagatgctga gtacaatact
    39781 cttaaattct tctggcaatt cgattgtttc agaaatttct gcaccaattt tttctacaaa
    39841 gaatcggcaa atcatctcta cgtcattgac ataaagcatc acacgtgttt tttgtatcat
    39901 catttgaccc ttctttctat ttgattttct ttcctgtcta taaactgatt ccattatatc
    39961 acgaaagcgc aaacaccttg tgtttgatac tattcaagaa aaataaagca atagtttcta
    40021 ggattgcttt cctccacaaa ctttttttac aaaaaaaaaa aacggcaatg aatgccgctc
    40081 ttcttatttc gattctttta taattctgct tctgattcat ctttggttag tgtgtgtcta
    40141 acactagtta cgccaaatgt ttgatcgatt ttttccttca acgcttcgat tgcctgtgcg
    40201 ccacgtgccg cttgttcagc attgatggat tcaatgacca gcaccgctgc ttgtgtttct
    40261 tccgtcagca ttgttctttg ggcttcacgc cagttgagac agcgacagat cgctccttgt
    40321 tcatcatagt agatgatttc ttccggaagt gccggagcat cggaatctgc acctaaaggc
    40381 aggaatgatt cgccaccttg ggcttttcct aaatgcagat ttcctgcgat cttttcgata
    40441 tcttctccgc cgcaaggtac gccaaaagac aaggaaatac tattatagat atctacaagc
    40501 ggattgattg ggaaaaattc ccgttcttga tttgcacgtt tcagtaaagc ttcaatagaa
    40561 gaacgtgcac cttttttagt cttgaatgtg gtaaatgcct gacgccactg agcaatgact
    40621 tcgttttggc tgaatgtttc ttctgtaagg taatttcttg cttcttttgc tgcctcattc
    40681 aataattgat gatacgtttc ttcactttct tttgttacgt ggttatcgat accatttacg
    40741 gataagattt cgatggttgc ttcaggaaat acttcccaga aattggtatc cactattact
    40801 tttttcatct gttcacacct cttgattaga ataattgctt gatgaataga tagtctgttt
    40861 ggggatcatc tcccatataa aaaacatgtt cgctgtattt gataaatcct tttttacgat
    40921 aaaaagcgag cgcaacatcg ttgtgttccc aaacaccgag ccaaatggct gttttttgct
    40981 tttcttttgc tttttcaata gctgtatcta gtaaaaatgt accgatacct tgatgtttaa
    41041 atttttttcg tacatagatt cgctcgattt ctaaaaaatg atcagccatt tcctcagatt
    41101 gactcgttcc tgtatttagt ttcaggtaac ccgcaggctc ttgatcgata ctcactagga
    41161 aaaaatcggt ttctttatct tttagctctt ctgtaagttt ttctagtgca taagcttgtg
    41221 tcaagtaatc agctaaatgc tccggtgaat tatcttttcc aaatgtatct gtaaatgttt
    41281 cgatactaat ttgttgtagt aagtctacat ctgaaggcgt catttttcga atttcgtatt
    41341 tattcacggc ttcttccttt caatagatcc ttttattgcc ttttttgaca aaattccaat
    41401 cttcgctaac attcttgcat actttattta aaagatattc caattgtttt gcttcttcgt
    41461 cagaaagacc atttaatgcg actgcattgg agtagttgtt ttctcgaatg atgaaaggat
    41521 agatttctgc tcctttttta gtcggataga gtttcttgat ttttttattt tctttatctt
    41581 ccaaacgttc gatcatccca ttggattcta atttcttgat tgctcgggca gtcgttgtac
    41641 gatctacttt gatcatttct gctagttttt cttggatgat tccaggattt tcgcaaatac
    41701 gtactaaata aagatactgt cctcttgtta aatcgacttc tttgaattcg atattgctaa
    41761 tagaatctag cgctcgggca atcattccga tgtctcgaag gatttcagcc atttcatccc
    41821 acctttattt tgtttgtaag actagtttac ctctttttgt tgcaaatgca actattttca
    41881 ataaaaaaat caagttcttt ttt
//
LOCUS       DACDKM010000016        34785 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20834_2016
            SAMN12640370-rid9891663.denovo.016, whole genome shotgun sequence.
ACCESSION   DACDKM010000016 DACDKM010000000
VERSION     DACDKM010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640370
            Sequence Read Archive: SRR10040891
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 34785)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34785)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742835.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742835.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:04:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,012
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 29 of 75
            Pseudo Genes (incomplete)         :: 33 of 75
            Pseudo Genes (internal stop)      :: 19 of 75
            Pseudo Genes (multiple problems)  :: 5 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34785
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640370-rid9891663.denovo.016"
                     /isolate="E20834_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            23..316
                     /locus_tag="IV334_000818"
     CDS             23..316
                     /locus_tag="IV334_000818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2087 domain-containing protein"
                     /protein_id="HAP8942911.1"
                     /translation="MVYTYTEQEKMKVLDTFMNDGKVKQVPSSTKKKYILLKEVLKRF
                     DHGVTYTETEVNSILLNVFSSGDYVEQRRYLITFGFFKRSSDGRAYQMMGIEN"
     gene            1159..3486
                     /locus_tag="IV334_000819"
     CDS             1159..3486
                     /locus_tag="IV334_000819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HAP8942912.1"
                     /translation="MKMIKKTQNKIQRICQKHFVSRLKAVSKRKYLHPIMISIILIFT
                     FLGSLSSIYVNVYTETSNFANQTTEETNSPPYSEIQSIESTNQSSNNETTSTTSSTQL
                     IDPTEKEITAAKAAGEKAYSETGRSQTFSTVAATANFNGHTIPDGYTVIYAKDGVIVA
                     VNGSMSADKTALQGVTKAIAFIDNRPDIHNLFIGSTVNGTTITFPKSIYMGVATAGNT
                     IDSVTPNADKSYSFTPSTWGGLPAGGGMSTFSIGIQLANDIPFTGYSIKNGQIVDGKG
                     NVSNFPTYQAATVQVNANRTGTFSYTVNGGAAKIGTYGTTLSGIHVGDKVVITPTAVT
                     GYSFAQTNGSFTALESNVDTVTYTGKQTPITIKYQDYFGQMIAPSKIMNLTYGSAAQD
                     LTTNTPTINGYTFTAVSAIETKNQSAMSVLASLDTNGNVIVKDANGKQISEVILYYKT
                     NATVSINANGSKYYDGLSVVPIVKYAFNDKTESTSLLNNDLSIDQITWNPTDFKAMNE
                     KGVEVTAPTEIGTYTSWQLTKSGLAKLAARTNYLFTIVQTSDVYTIKQISGAVELSGS
                     KTYDGKAGVPSIHVKLAEGVTSSLTPVQVALSSMDYTADAQSAKNMVNAGSYTIKLTN
                     SGIDKVKAANPKLSFTDIANTSGTYTIYKANAVITVDDAAFNYDAQSHSISVGNLHVT
                     GVVLGESLDYTLTVNSRTDIGSQIVTISLGEGSVNNNYTIIATDTAKLTINPTLLLPS
                     TGGIGLLPFVFLGLIFIFSGFFYFIHRKKKAGEQR"
     gene            3612..3941
                     /locus_tag="IV334_000820"
     CDS             3612..3941
                     /locus_tag="IV334_000820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall anchor protein"
                     /protein_id="HAP8942913.1"
                     /translation="MFESDKMPSISQNDGINIPDNFQGVKGLNGVTFAIYDVSDEFYK
                     LRSEGSSVEDAQRKLAQKSDSEKILAENVTKTVDEEEGIASFSASDKDEQGRDAVYRF
                     TEIKTSD"
     gene            3981..4985
                     /locus_tag="IV334_000821"
     CDS             3981..4985
                     /locus_tag="IV334_000821"
                     /inference="COORDINATES: protein
                     motif:HMM:NF037639.1,HMM:TIGR04226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopeptide-forming domain-containing fimbrial
                     protein"
                     /protein_id="HAP8942914.1"
                     /translation="MTDSSNHKLTTIHLYPKSEQKIPAALTLTKTVENKQTDFADGDK
                     VPYLITVTIPENINEIGTYTIKDTADPQLWLEPETIDFLIGGDKIHTFHTQKIKHGFV
                     LSDSGKDLSSFAGKKLTIRYQMTLKENSEKTTFDNDVRLTTDNQTVETHLAIQTGGKQ
                     FVKVDRQTKQKLADAQFLVKKNDQYLAQENGINHWVAKDDSRATIFTSSKDGTFSVHG
                     LSFGSYELVEIKPPKGYILSQSTILFEVEKGSYDPLHPIPLEIINEPKANPTNNGKTP
                     VQMQTNGPSNEKNGGSFPKTSEEMLGGFSILGLLLVLSTGTAWFYKKQQKGNNRREIK
                     "
     gene            4982..5428
                     /locus_tag="IV334_000822"
     CDS             4982..5428
                     /locus_tag="IV334_000822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall anchor protein"
                     /protein_id="HAP8942915.1"
                     /translation="MKNIKTKIWTFLGTAIMLLPFVLGLGTAEVSAAVSPTPENVTVN
                     LHKLKFTSAPENQINNGTELTFPNSEPLNGVEFNVYDITATYYPSKDTAVPADATPFA
                     SVTTSGEGLANLTLPGKSDGKDAVYVFVETPKPGVETSPNIVLSLP"
     gene            5578..6393
                     /locus_tag="IV334_000823"
     CDS             5578..6393
                     /locus_tag="IV334_000823"
                     /inference="COORDINATES: protein motif:HMM:NF033902.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HAP8942916.1"
                     /translation="MTGFDENTGVALYDPAESALTFTTDTEGSISVNGLIDGNYTLVE
                     TKAPDGYQLTNPQKETTFIVKNGKLQQTTGIIHGADEANLGNPNGVVNRENTTTDNTI
                     TVENVQNYGDFHFIKQDVNTQEKLADAEFSILRAKDPTVKLMKKIANLASGEYPYAWS
                     DEVTGSDWEEVKLISDSEGKFSIEGLKQGAYCLQETKAPEGYALPKNEAAYTEFTVDD
                     DPTTSDETTIDNQPKGILPSTGGMGIIVFILVGTALVGGAVIYFKKRHQETEA"
     gene            6599..7771
                     /locus_tag="IV334_000824"
     CDS             6599..7771
                     /locus_tag="IV334_000824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HAP8942917.1"
                     /translation="MNKSRIRILDIFMAIILVVGIGIFSYPFVEDSLNDFLAQQMIIH
                     YQKQASNKNSEEIKKQQEKMTKKNQQLAEQNVSPGIGSFNQAVDAKALKDLPSNAFFM
                     EHMLGVIEIPKINVSLPIFDQTTEIFLQKGTSLLEGSSYPTGGKNTHAVLSGHRGLPE
                     AKLFTDLPKLKKGDQFFIQINGKTLAYQVEKIQVVLPDEVDSLGIQKGRDLVTLLTCT
                     PYMVNTHRLLVTGHRIPYQAKEAKKAIQGIDQWKKWKFFIWFIGILLGSIGLVWLLIA
                     YLDSLAIAKRNYPLSFYVKNTNGRPIEGMVFSVKTLNGKHYITREKVPFVKASDEYGL
                     VRFSDLKGRNYRLQHEELLLKIHVKHKHSKQFSMKLKKGRYKLRKEKEVYYLIEKE"
     gene            complement(7933..8427)
                     /locus_tag="IV334_000825"
     CDS             complement(7933..8427)
                     /locus_tag="IV334_000825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8942918.1"
                     /translation="MTEAESALSQAEANPTKENYATASAAIQALPENKQELTSRLAAI
                     DSTIKAKEAEEERKKQEEIARQQAEQAQQEAIAQQQAEQARQEAIAQQQAEQARQQAA
                     AEQASQQAQAAAPEQNNEQTVYVTPTGSKYHTHKCGNGTYTPATLSEAQARGLTPCSK
                     CYGN"
     gene            9010..9432
                     /locus_tag="IV334_000826"
     CDS             9010..9432
                     /locus_tag="IV334_000826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HAP8942919.1"
                     /translation="MQTFLTKVSQKKIKMIHLLLETERWCSIEELQNELKVSSKSILN
                     YLTDLEELFQQYPDKVILKNENNRRFSMEKQENFPIYIIYLHFYRKSYNYHLIEFMYQ
                     HPEKNLEDYADAQYTSVSTVFRYAKLLVKYFEMTDVKE"
     gene            complement(9598..10791)
                     /locus_tag="IV334_000827"
     CDS             complement(9598..10791)
                     /locus_tag="IV334_000827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP8942920.1"
                     /translation="MTTYQRKVLVSTSAGIALENMDIMFLAFSLSSMITSFHISGTQA
                     GFISTITNLGMLIGGIFFGIIADKYGRVKVFSQTVLLFSIASLLMYFASNIYLVYLFR
                     FIAGVGAGGEYGACMSLISESFSKKQIGRASSVAGIGAQVGAALAAILAAVIIPTFGW
                     KMLYVVGVLPVLMVLFIRRGLTEPKEFKSVQSAEKKTKLSRLFESKHLAWQTCGLSIM
                     VTVQIAGYFGLMNWLPSIMQNRLGLSVSGSSLWMVSTILGMSLGMMTFGIIMDKLGPK
                     LAFSLFLICSSLSVYLLVLAHSQWSLVAAAVIVGYFINGMYGGYGAVISSLYPTEIRA
                     TANNFVMNLGRAVGGFSSIVIGFLMDHYSLTAVILFLSGIYLVSLLVLTTLTGVTALR
                     ESLQH"
     gene            11015..11392
                     /locus_tag="IV334_000828"
     CDS             11015..11392
                     /locus_tag="IV334_000828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HAP8942921.1"
                     /translation="MNIKQVSEEKGISADTLRYYERIGLIPPVNRTKGGIRDYTEEDL
                     KWVDFTICMRNAGLSIESLIEYIRLSSEGEATVFERRKLLIEESELLTKKIAEMQECL
                     ERLQGKIKKYDRVLSRNKMECIK"
     gene            11380..11718
                     /locus_tag="IV334_000829"
                     /pseudo
     CDS             11380..11718
                     /locus_tag="IV334_000829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321036.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
     gene            11852..12295
                     /locus_tag="IV334_000830"
     CDS             11852..12295
                     /locus_tag="IV334_000830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator
                     substrate-binding protein"
                     /protein_id="HAP8942922.1"
                     /translation="MLDPVDTEKYETILLPRKEKWGLLILENSVLAKKDVITKEDFAN
                     VPLLISKRPEIQRMFADWLGIKKEQLLITGTYNLIFNVFALIENQVGAALVIEGAVSH
                     HSKRVTFVPLDPMIETNCMLVWKKNRILSPVVNELIHRFKEAFEQ"
     gene            12556..13371
                     /locus_tag="IV334_000831"
     CDS             12556..13371
                     /locus_tag="IV334_000831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010731016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAP8942923.1"
                     /translation="MPFIQWLGMILLIVLALLTIIPFLWMVSASFKANNEVFTIPIQW
                     IPKTWHLENYLVIWSRIPLVTFFKNTAFLSVVITLIQLFTSSFAAYGFSKLNFKGRDI
                     LFLAYIGTIAVPWQSYMIPQFIMMRKVGLTDTLWSLVLLQAFNAFGVFLLKQYYSSIP
                     DSLCESARIDGLSEWGIYRKIILPLTKPAIASLTIITFVNTWNDYMGPFIYLSSTENK
                     TIQLGLKMFIGLFDAEYALIMAASVVSVLPVAFIFLAMQKYFVEGIASSGMKE"
     gene            13381..14043
                     /locus_tag="IV334_000832"
     CDS             13381..14043
                     /locus_tag="IV334_000832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HAP8942924.1"
                     /translation="MKEQRFDKNVYMEFFYWLYQSLASSLCFWCVNILYIAAAFLLSI
                     DGRNVFSFIFSLLFIGPGCIVMIGMTEELAKENSFSPVRLFIKMFRQFWLKGFFYWLF
                     AWIVSVIMIFDCFFFIRFSYGKWLIPLFVLLACLSVSFSTNCWYFQVRNPASKPNQVL
                     RIAFYYTLKKWYVSLLDFLLLTSLFLFFFVKPQWCILLGPSIVFGLIYFNNRKLMRTM
                     DL"
     gene            14251..15915
                     /gene="alsS"
                     /locus_tag="IV334_000833"
     CDS             14251..15915
                     /gene="alsS"
                     /locus_tag="IV334_000833"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase AlsS"
                     /protein_id="HAP8942925.1"
                     /translation="MAEKMKQGSKSVVESLINHHVDYVFGIPGAKIDGVFNELEDQGP
                     ELIVTRHEQNAAFMAQAIGRITGEPGVVIATSGPGASNLATGLVTATAEGDPVLAIAG
                     QVKRSDLLKLTHQSMDNAALFQPITKYSAEIQDPETISEVIANAYRMAKSSKKGASFI
                     SIPQDVVDAPVQGNVIKPLSDPKLGSASADDIRYLAERIREAKLPVLLVGMRGSSEKE
                     TLAIRQLVEKTALPVVETFQAAGVISRELEAHFFGRVGLFRNQPGDMLLKRSDLVIAI
                     GYDPIEYEARNWNAEKDARIIVIDEAPAEIDPFMQPERELIGDISATLDLLTGSLEPQ
                     QVSEDAKEYLASLQAKLTERDIVQSKGEAGILHPLEVINTLQSKVTDDMTVTVDVGSH
                     YIWMARHFRSYEPRHLLFSNGMQTLGVALPWAISAALVRPNTQIVSVSGDGGFLFSAQ
                     DLETAVRKKLNIVHLIWNDGHYNMVEFQEKMKYQRASGVDFGPVDFVKYAEAFGAKGI
                     RATSVEELEKALEEGFATEGPVIIDIPIDYRDNEKLGETILPDQFY"
     gene            15928..16638
                     /gene="budA"
                     /locus_tag="IV334_000834"
     CDS             15928..16638
                     /gene="budA"
                     /locus_tag="IV334_000834"
                     /EC_number="4.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate decarboxylase"
                     /protein_id="HAP8942926.1"
                     /translation="MTEKILYQHGTLGALMAGLMDGTETIAHILEKGTLGLGTLHGLD
                     GEVIFLDGVAYQGRSDGSVAQLDGSELTPYAAITDFTPDTSFSVSKTADGEQLKKDIL
                     VREAGENLFLAVKITGLFKNMHIRIMPKQEKPYRRLAKISESQPEFQQSNIQGTLVGF
                     FTPELFQGVAAAGFHLHFIDDTHTFGGHVMDFEVAEGKVEISRISSLVQHFPVDDPTF
                     VHETIDYADLAAEIQQAE"
     gene            16768..17265
                     /locus_tag="IV334_000835"
     CDS             16768..17265
                     /locus_tag="IV334_000835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HAP8942927.1"
                     /translation="MWTEKQKKDAYEILLLQQQGLLEAEDNWLANLANSSALLNETLP
                     ETVFAGYYLFDGNELILGPFQGRVSCTRIKMGKGVCGESAEKQETLIVDDVKTHANYI
                     SCDSAAQSEIVVPMVKEGNLIGVLDIDCGVTKGYDEIDQAFLEKYVDLLLANFNINAF
                     SFINN"
     gene            17450..17710
                     /locus_tag="IV334_000836"
     CDS             17450..17710
                     /locus_tag="IV334_000836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8942928.1"
                     /translation="MNNETIILGKKYICQPIGLKQKVVGEVISKLENCVVICVDEYHD
                     VDRNEILEKCGKVVAKYTHVYELETTEYLYNYSNKYEHTLVS"
     gene            complement(18072..18311)
                     /locus_tag="IV334_000837"
     CDS             complement(18072..18311)
                     /locus_tag="IV334_000837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8942929.1"
                     /translation="MKRILFISLSNLLLLTACGTSASKNIPSDTQISNSIDSTISTNE
                     KNSPVETSDIKVSTSAKNRVKKLSARLLYFKQTLN"
     gene            18597..18911
                     /locus_tag="IV334_000838"
     CDS             18597..18911
                     /locus_tag="IV334_000838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8942930.1"
                     /translation="MDFYSNFILIIAILLLLNIWFFDKSRNAGIGFRTKRSTSSEKKW
                     VYSQTIFYGGVISISLLSSTLYSFNVIDVSMSNFISIIGILISAIITQLLLVFEEKSK
                     NN"
     gene            complement(19345..19711)
                     /gene="ssrA"
                     /locus_tag="IV334_000839"
     tmRNA           complement(19345..19711)
                     /gene="ssrA"
                     /locus_tag="IV334_000839"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            complement(19882..20655)
                     /locus_tag="IV334_000840"
     CDS             complement(19882..20655)
                     /locus_tag="IV334_000840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAP8942931.1"
                     /translation="MSNYRAITFFDLDGTLLDAKSKITPEVAKAMNALKENNVLPVIA
                     TGRTEAEIHSIMEDAGITSAVTMNGSFIRVDGEEIYSQTFSSEECQKMYDHVQLNGHE
                     LSYYNDQKIWCTGHSQTMINAYDFIHSDVPEIDPLGFKENTVNMLLILSQSGDEYYHE
                     HFPELTFYRNGPYSIDTVKKGISKGAGVKRLQKELQLEKVPTFGFGDGPNDFALLEAC
                     DNKIAMGNAYDGLKELSTFITKKNTEGGIVHALKYFDLI"
     gene            20799..21149
                     /locus_tag="IV334_000841"
     CDS             20799..21149
                     /locus_tag="IV334_000841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdpI family protein"
                     /protein_id="HAP8942932.1"
                     /translation="MIFFSIGILFLIIGSLFRILPSKGNLPFYGYHSPLAAKTDAHWR
                     LAQKTSGNWFFLMGLLMALIGYYLKTSGHTNYFLIEMLLLVFPIMPIFIMTEKKLQKY
                     DLETGGNDNEYFND"
     gene            21130..21846
                     /locus_tag="IV334_000842"
     CDS             21130..21846
                     /locus_tag="IV334_000842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP8942933.1"
                     /translation="MNILMIEDNQSVSEMMQMFFLNEGWEATFKYDGKEGLDAFLENP
                     QHWDMITLDLNLPTLDGMLVGREIRKVSSTVPIIMLTARDSESDQVIGLEMGADDYVT
                     KPFSPLTLIARMKALHRRSELAEAREEAAVSDDHFDIETSHFKMNTKTREAYLDNRPI
                     EGLTPKEFDLLFTLAKKPRQVFSREQLLELVWDYQYFGDERTVDAHIKKLRQKIEKVG
                     PQVIQTVWGVGYKFDDSGVA"
     gene            21846..23294
                     /locus_tag="IV334_000843"
     CDS             21846..23294
                     /locus_tag="IV334_000843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAP8942934.1"
                     /translation="MRYLYQQLLAFCGMVALILLIVGVSFTQLTKQTIEENNYQQLFG
                     YAESVEKTTQTYADTLPQFSQDQAFQNALLLTEQVLTEQNVNFIFIDKNERVIYPTNT
                     ALLNFTLTADQWKNLRNGRQEKFTSNKNILGQAQATSYALVPFNLNHEFYGALVVSQP
                     ARNIDNSVRPITLNLFKGFIFSSIVAVIASYAFAAFQVKRINRLRNATKEVTNGNFDV
                     QLPVHDKDEFDELADDFNKMTNSLKESQAEIEEQENRRRQFMADASHEMRTPLTTING
                     LLEGLEYNAIPENQRENAIKLMKNETERLIRLVNENLDYEKIRTNQISMVIKKFNGTE
                     TLKNIVAQLEAKAEAAGDTLTLKAVDDIDVYADYDRFVQIMVNIIQNAIQFTENGQIT
                     ITLEKGYLETIITIEDTGIGMSEQQMKSIWDRYYKVDPSRKNTKYGESGLGLPIVQQL
                     VRLHKGKLEVESELGKGTKFAVRLPDVEIKKE"
     gene            complement(23364..23873)
                     /locus_tag="IV334_000844"
     CDS             complement(23364..23873)
                     /locus_tag="IV334_000844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8942935.1"
                     /translation="MKPEYLIHAEHLTVILCPLEKQHKILEHLKKEVLVAKLDNFAII
                     QKNWPMFPYLNLKDHVLLDVPEKEIKQDRLAYQEKLDISPSLLNCAAAELTSFEKVKL
                     QLLHALLSKRNNIVIEDTFDELSVLEIQELLHLLSYLAHEENQGILLFTHDATIAQSP
                     YIDRLEPAG"
     gene            complement(23863..24588)
                     /locus_tag="IV334_000845"
     CDS             complement(23863..24588)
                     /locus_tag="IV334_000845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8942936.1"
                     /translation="MKIIRYATARLIYYKKQTILYCLFSSFSAFLLIACLTLYHLQSA
                     LANQVQDRLSFIGASASTELLPALELASRFYIKGIFSLFFLFAFLFIFFFYYSVRQNK
                     QELINWRLTGLSKTKLFLFIFWQLFFPLFLCCILVLLWIVSFQSVYEALLQRINFLVL
                     KTTELPDIRDILTSVSGLTIPMDQQTFFTIDFQNDLFFTDILKGFAQTLIGLSGCCLI
                     LSLFPFTFFIHHLQSRRPGRNET"
     gene            complement(24811..26931)
                     /locus_tag="IV334_000846"
     CDS             complement(24811..26931)
                     /locus_tag="IV334_000846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HAP8942937.1"
                     /translation="MKCIKTPAFLNKRLGFFSLLVVLLWLKTIFAYLVDFHLGIESGI
                     QYFILFINPIATTLLLLGIALYIRRPRVAYITMMVIYFLLTLLVFSNVTYYREFTDFI
                     TINTILGAGKVASGLGESAIRLFRPYDVIYWIDFIILTVLLIAKKIKLDPRPIRARMA
                     FAISTLAVMIFSGNLFMAEADRPELLTRTFSRDYLVKYLGLNAFMVYDGVQTYQTNQV
                     RAEASPNDMKEVENYVKEHYAAPNDDLFGLAKGKNVIYIHLESFQQFLIDYKLTDENG
                     LQHEVTPFINSLYHSNSTFSFDNFFHQVKAGKTSDAETLMENSLFGLNQGSLFTQLGG
                     KNTFQAAPDILSQTQGYTSAVFHGNAGTFWNRNETYKNLGYDYFFDSSYYDVNDDNSF
                     QYGLHDKPFFQQSVQYLERLQQPFYSKFIAVSNHYPYSKFTNDEAGFPLANTNDETIN
                     GYFATANYLDTAVQEFFNYLKESGLYDNSVIVLYGDHYGVSNSRNKNLAELVGKTSST
                     WTNYDNAQMQRVPYMIHIPGQTQGGINHTYGGQVDALPTLLHLLGVDTKNYIQLGQDL
                     FSKDHEQLVSFRDGDFVTPKYTYYGGTIYSNETGEAITEPTEEVQNEIDSLKEKVDKQ
                     LAVSDQINNGDLLRFYTNSGLDPIDTSDYDYKNGLEKLKEAEKSLGDKSTSVFSEHNG
                     QSTVDLYKTQTYQELHGTTTSSSE"
     gene            27161..28339
                     /locus_tag="IV334_000847"
     CDS             27161..28339
                     /locus_tag="IV334_000847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent rRNA methyltransferase"
                     /protein_id="HAP8942938.1"
                     /translation="MKVKVKKRASNRLKKGYPLIQKEDLAHPQKETTQWVELVDSQNQ
                     FIAKGYLGKQNKGIGWVLSQQNIPFDQSFFEEFFVKSKEKRTFFFSDDSTTAFRIFNG
                     EGDGIGGLIVDYYAGYAVFSWYNETLYAHRELLIQAFREIYPEIVGGYEKIRFESVNL
                     PESQFIYGKEAPEPLIVKENGVKFAVYLNEGLMTGIFLDQKEVRGSLAEGFASGKTVL
                     NMFSYTGAFSVAAAMGGAIETTSVDLAKRSLKKTQEQFEVNGLDVDQQKIIVMDVFEY
                     FKYAKRKQFLYDMIVLDPPSFARNKKKVFRVAKDYGELVKDSLDILAEDGILIASTNA
                     ANVSIEQFQTMIEEEFHAKNVSFEQLALYRLPKDFQTTDTFLEGNYLKVFVYQIKNNH
                     "
     gene            28355..29113
                     /gene="aroD"
                     /locus_tag="IV334_000848"
     CDS             28355..29113
                     /gene="aroD"
                     /locus_tag="IV334_000848"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HAP8942939.1"
                     /translation="MVWVKGLNIGSGRPKICAPLIGAVREEVLREAVLAREAGADLVE
                     WRVDHYREVFSFDEVAETLVLIHEMLDGLPLLFTFRTLAEGGEQDISIRQYRDLYEHV
                     IHTGLVDMVDIELFKVESLGKRLIEEIKSLNIPLIISSHDFKETPADPVLLYRLNVME
                     HFGADIGKLAVTPNNERDVLRLMELTRRANAFVSMPIITMSMGNLGKISRLAGGTTGS
                     VMTFGSLSSDSTSAPGQLPVKALKQIIQLLNEPL"
     gene            29136..29621
                     /gene="ybaK"
                     /locus_tag="IV334_000849"
     CDS             29136..29621
                     /gene="ybaK"
                     /locus_tag="IV334_000849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro) deacylase"
                     /protein_id="HAP8942940.1"
                     /translation="MAKKKIVKTNAIRMVEQKKIPYTEHEYEWDESHLSASSVAEQLP
                     ESQSRIFKTLVAVGNVTGPLVAVIPGEAELNLKKLAKVSGNKKVEMLHLKDLEATTGY
                     IRGGCSPIGMKKLFPTYLDQIAESYEQIIVSAGRRGLQMELAPQDICALTSGQFADIK
                     Q"
     gene            complement(29692..30945)
                     /locus_tag="IV334_000850"
     CDS             complement(29692..30945)
                     /locus_tag="IV334_000850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HAP8942941.1"
                     /translation="MEKLYDVIVVGAGTSGMMAAISAAEQGARVLLIEKNKKAGKKLL
                     MTGGGRCNVTNNRPVDDLIAHIPGNGKFLYSTFAQWNNFDIMNFFESQGVHLKEEDHG
                     RMFPVTNKSKTIIEALLNRLKELDVTLLFSTRVEKLIHKEHKIYGIRTEFEEFHAPAI
                     ILTTGGRTYPSTGSTGDGYKIVKRVGHTVTPLYATESPLISDEPYIQEKTLQGLSLQD
                     ITLRVLNKKGRVLTEHTMDLLFTHFGISGPAALRCSSFVNKELEKTGEPVTLSLDCFP
                     TQTKQELIHILTEKSKATKKNLVNAWHGILPERLLVFFLERLEMDHLTGQQASQKQIQ
                     DFVQLCKEFKLSINKTFPIEKSFVTGGGVSLKEIYPKTLESKIIDGLYFAGELLDVNG
                     YTGGFNITAAFATGHVAGMNAGQRA"
     gene            31063..32412
                     /gene="gdhA"
                     /locus_tag="IV334_000851"
     CDS             31063..32412
                     /gene="gdhA"
                     /locus_tag="IV334_000851"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HAP8942942.1"
                     /translation="MTNATEYVQAIQEKLHQKDQGQEEFLQAIDEFMPTVMSFLDTHP
                     EYIEKNILELLTEPERVIQFRVPWQDDEGNWRVNRGYRIQYNSAIGPYKGGLRFHPSV
                     NLSILKFLAFEQIFKNSLTGLPIGGGKGGSDFDPKGKSDNEIMRFCQSFMLELSKHIG
                     PSIDVPAGDIGVGAREIGYLFGEYKRLKKYDTGVLTGKPLDFWGSKIRTEATGYGLVY
                     YVKHLLNEEKDSFNQKTVFVSGSGNVAIYAIEKVQELGGKVVTCSDSSGYIYDPEGID
                     VKLLKEVKEINRQRLTEYANQRPSAIYHAGESVWTLKEKADIALPCATQNEIDESLAE
                     ILVENGIKIVAEGANMPCTVKAVSVLHEAGVWYCPGKAANAGGVAVSALEMSQNVQRL
                     TWEREQVDEQLDQIMETIYHTCRQTAAEYGDEKNLLLGANVAGFEKVAKAMAAEGLV"
     gene            32524..33870
                     /locus_tag="IV334_000852"
     CDS             32524..33870
                     /locus_tag="IV334_000852"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HAP8942943.1"
                     /translation="MAHIHFDYSKVAPFVNEQELGYMQSQVTAAHNELREGTGAGSDF
                     RGWIDLPTNYDKEEFARIKEAAKKIQSDSEVLVVIGIGGSYLGARAAIDFLNHTFFNL
                     LDSNTRKAPQIFFAGNSISSTYIADLIEVIGDRDFSVNVISKSGTTTEPAIAFRVFKE
                     LLVKKYGQEEANKRIYATTDKAKGAVKVEADAEGWETFVIPDDIGGRFSVLTAVGLLP
                     IAVSGADIDALMQGAADASKAYSSDKLEENEAYQYAAMRNILYRKGKVTELLINYEPG
                     MQYFSEWWKQLYGESEGKDQKGIYPSSANFSTDLHSLGQFIQEGRRNIFETVVKVEKP
                     RKSITIPEQAEDLDGLGYLQGKEVDFVNTKAFEGTLLAHTDGDVPNLLVKIPEMDAYT
                     LGYTMYFFEIAVGISGYLNGINPFDQPGVEAYKKNMFALLGKPGFEDLAKELNERL"
     gene            complement(34178..34564)
                     /locus_tag="IV334_000853"
     CDS             complement(34178..34564)
                     /locus_tag="IV334_000853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HAP8942944.1"
                     /translation="MKKILGLIVIILIAFIGWKGWDYYQSTYVGKDYYAVVKAPMPAE
                     TDIKADNGEVIGRGFKYNVDAYAENGEKRQLDFDVITSGDSANGSAYPEGTILQLKAS
                     EKRIIEKKVITSDKVPSSVKEKLGLS"
     gene            complement(34613..>34785)
                     /locus_tag="IV334_000854"
     CDS             complement(34613..>34785)
                     /locus_tag="IV334_000854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS982 family transposase"
                     /protein_id="HAP8942945.1"
                     /translation="VETVFSSLEKLGCQNFNSRSVKGLESRFESILLAYSVLLSRAQR
                     RFEGTLRYSLGY"
BASE COUNT        11631 a         6086 c         6878 g        10190 t
ORIGIN      
        1 tagaatcaaa aggagaaaga agatggttta tacttatacg gagcaagaaa agatgaaagt
       61 tttagacaca ttcatgaatg atggaaaagt aaaacaagta ccaagttcaa caaaaaagaa
      121 atacatccta ttgaaagaag tcttgaaacg ctttgaccat ggagtgactt atacagaaac
      181 agaggttaat tcgatactgc tgaacgtatt ttctagtggc gattatgtcg aacaaagacg
      241 ttatctaatc acatttggct ttttcaaaag aagttcagac ggtcgggcat atcagatgat
      301 gggaattgaa aattagaagt tttaatttat actcattgaa aaaagttcaa gtatgcgata
      361 ttggttctac accgcatact tgaacttttt ttgtcatctg tatctataat acaaagccaa
      421 gaacgttatt caaaatatgg atagctatga taaaagctaa tgagcgattc gcagttaaat
      481 aagcaatcgt cagtaaaatc gttccaggag cgtacatgaa aaaatcaatt ggatgcgtta
      541 catgaataaa gataattctc ttttatttgt acgaataaat accaaaacgt acctcctaaa
      601 attagatttt ggacagatgc cctttaaaaa gataacaaga tttcgttctt gaaacaatat
      661 atatgctttt attccaatga ggttgtgaaa acagtatttt gtcctgagcg ttagaaaatt
      721 gaaaatgggg aatagcaact cgtactttac cattttttaa gtgaatacaa gatcagctaa
      781 ttgaacaccg tttattcgtg atgagggaca caacactgca gtcgacacat ttctctttta
      841 tgttcaaaga taacaaatat ttaaaacaaa gaaaaatagt agataatccc gtcctaactc
      901 ttttattctt ccctcatagc ttccaaattt aagcaatact tcaattaatc aagttttatc
      961 tttttttttc aataatcgta aaaaaagagt attttttatt taaaaataaa atctttgtat
     1021 taaaggacaa gactaaaata accttagaat aaaaagagaa aaaaagcccc aaactttcta
     1081 tacatcaata aaataataaa aattaaattt agaataacgt gtaaaggatt atttgatgat
     1141 tttttattga gaggagggat gaaaatgatt aagaaaactc aaaataaaat ccaacgtata
     1201 tgtcaaaaac attttgtttc tagactcaaa gctgtatcta aaagaaaata cttacatccc
     1261 attatgattt cgatcatctt gatttttact ttcttaggga gcttatcaag tatttatgta
     1321 aacgtttata cagaaacgtc aaattttgct aaccaaacga cagaggagac aaatagtcca
     1381 ccctattctg aaatacagtc catagaaagc accaatcagt ctagcaacaa cgagacaacc
     1441 tcaaccacat cctcgactca actgattgat ccgacagaaa aagaaatcac agcagcaaaa
     1501 gcagcagggg agaaagctta ttcagaaaca ggtagaagcc agacatttag tactgttgcg
     1561 gccactgcga atttcaacgg acataccata ccagatggat atacagtgat atatgccaaa
     1621 gatggagtca ttgttgcggt taacggatcg atgagtgcgg ataaaactgc gttacaaggc
     1681 gtaaccaaag caatcgcttt tattgataac cgtccagata tccataatct gtttattggc
     1741 tccacagtaa atggtactac tatcacattc ccaaaaagta tctatatggg ggtggctacc
     1801 gctggaaata cgatcgattc agttacaccc aatgcggata aaagttattc ttttacaccc
     1861 tcaacatggg gagggcttcc tgccggagga ggtatgagta ctttttcaat cggtatacag
     1921 ttagcaaatg atattccatt cacggggtac tcgatcaaaa atgggcagat cgttgatggt
     1981 aagggaaatg tttcaaattt tccaacctat caagcagcaa cggttcaggt caatgcgaat
     2041 cggacaggaa ctttttctta tacagtcaat ggtggagcag caaaaatcgg aacgtacggg
     2101 actacattat ctggaatcca tgtgggtgac aaagtggtta tcactccaac agcagtaacg
     2161 ggctattctt ttgcacaaac aaacggttct ttcacagccc tagaaagtaa cgtcgatacg
     2221 gtgacctata caggcaaaca aacaccaatc actattaagt atcaagacta ttttggacag
     2281 atgattgctc cttctaaaat aatgaatctt acttacggaa gtgctgcaca agacctgacg
     2341 actaatacgc ctactatcaa cggatatacc tttacagctg tttcagcgat agaaacaaag
     2401 aaccaatcag caatgagtgt tttagctagt ttagatacaa atggaaatgt aatcgtaaag
     2461 gacgcaaatg gaaaacagat ttctgaagtc atcctatatt ataagacaaa tgcaacagta
     2521 tcaatcaatg caaatggcag taagtactat gatggtcttt ctgttgtacc gatcgttaaa
     2581 tatgccttta atgataaaac agaatcaaca tcccttttaa acaatgatct cagtattgat
     2641 caaatcacat ggaatccaac tgatttcaaa gcgatgaatg aaaagggagt tgaagtaact
     2701 gcgcctactg aaattggaac ctatacgtct tggcaattga caaaaagcgg actagctaaa
     2761 ctagctgcca gaacaaacta tttatttact atcgtgcaga catctgatgt ctatacgatt
     2821 aagcaaatca gcggtgcggt agagctcagt ggcagtaaga catatgatgg aaaagcaggt
     2881 gtcccgtcaa tccatgtcaa gctagcagag ggtgtcacta gttcgcttac tccagtacaa
     2941 gtggcactta gcagtatgga ttacacagca gatgcacaat cagcaaaaaa tatggttaat
     3001 gctggcagct atacaattaa actaacgaat agcggaatag acaaagtcaa agcagcaaat
     3061 cccaagttgt catttactga tatagccaac acatcaggaa cttataccat ctataaagct
     3121 aacgcagtga ttactgtcga tgacgctgcc tttaattatg atgcccaaag tcactcgatt
     3181 tcggttggaa atctccacgt taccggtgtt gtcttaggcg aatcattgga ttatacccta
     3241 acagttaaca gccggacaga tattggatca caaatagtaa cgattagttt aggcgaagga
     3301 agtgtaaaca ataattatac gattatcgca acagataccg ctaagttgac gatcaatccg
     3361 actctcttgc ttccttcgac aggtgggata ggtctactgc cctttgtctt tttaggactg
     3421 atctttattt tttctggctt cttttatttt atccatcgta agaaaaaagc aggtgaacag
     3481 cgatgaaaaa aacaaaaaat gtacgtgtgg atatatttgc tgttctcttt tttatcctac
     3541 tggtcttttt tataggttct ccgactgtgt cagctagaga atcacctatt acatttactt
     3601 tgcataatcg aatgttcgaa agcgataaga tgccaagtat cagtcaaaac gacggaataa
     3661 atatcccgga taactttcaa ggagtaaaag ggttgaacgg cgttactttc gctatatatg
     3721 atgtctcgga tgaattttac aagctgcggt cagaaggaag tagtgtggaa gacgcacaaa
     3781 gaaaactagc acaaaaaagt gatagtgaaa aaatactggc agaaaatgtg acaaaaacag
     3841 tagacgagga agagggcatt gcttctttta gtgcatcgga caaagatgag caaggtcgtg
     3901 acgctgttta ccgatttacg gaaataaaga cttcagatta agtaaaagaa aaatcagcac
     3961 catttgtcgt tgtgcttcct gtgacagatt catctaatca taaattgacg accatccact
     4021 tgtatccaaa gagcgaacaa aaaatacctg ctgcactcac attaacaaaa acagtagaga
     4081 ataagcagac agattttgca gatggggata aagtccctta tctcatcacc gtaacaattc
     4141 ctgagaacat caacgagata ggaacctata cgatcaaaga tacagctgat cctcagttat
     4201 ggctagaacc agaaacgatc gattttttga ttggtggaga caagattcat acatttcaca
     4261 cacaaaaaat aaaacatggt tttgtcttat cagattctgg aaaagatctt agttcgtttg
     4321 ctggaaaaaa attaacgatc cgttaccaaa tgacattgaa agaaaacagt gaaaaaacga
     4381 catttgataa cgatgtacgt ttgacgactg acaatcaaac agtagaaaca catcttgcca
     4441 tccaaacagg aggcaaacaa tttgtcaaag tggatcggca aacgaaacaa aaactagcag
     4501 atgctcaatt tctagtcaaa aaaaatgacc aatacttagc gcaagaaaac gggataaatc
     4561 attgggtagc caaagatgat tcaagagcaa cgatttttac ttccagtaaa gatggaacat
     4621 tttctgtcca tggattgtct tttggaagtt acgaactagt ggagattaaa ccgcctaaag
     4681 gatatatatt gagtcaatca accattctct ttgaagttga aaaaggcagt tatgatccgc
     4741 ttcatcctat accattggaa atcatcaacg aaccaaaagc aaatccgacg aataatggaa
     4801 aaacgccagt tcagatgcag acaaatggtc catcaaatga aaaaaatgga ggaagctttc
     4861 caaaaacaag cgaagagatg cttggaggtt tctcaatttt agggttattg ctagtccttt
     4921 caacagggac agcttggttt tataaaaaac aacaaaaagg aaacaatagg agggaaataa
     4981 aatgaaaaac atcaaaacaa aaatatggac gtttttagga actgctatca tgctgcttcc
     5041 tttcgttctc ggcctaggaa ctgcagaggt ttctgcagcc gtatcaccta caccagaaaa
     5101 tgttacggtt aatttgcata aattgaaatt cacttcagca ccagaaaatc aaataaacaa
     5161 cggaacggaa ttgacatttc cgaatagcga accgttgaac ggcgttgaat tcaacgtata
     5221 tgacatcact gctacgtact atccaagtaa agacacagca gttccagcag acgctacacc
     5281 cttcgcaagt gtaacgactt ctggtgaagg attagctaac ctgactcttc caggtaaatc
     5341 tgatgggaaa gatgctgtat acgtctttgt agaaacacca aagcccggtg tcgaaacgtc
     5401 gccaaatatc gtgttaagct taccctagac cctgacaaac ctttagacac gattcaccta
     5461 tatgctaaaa atgtcatcaa agaaagcaat atcaaaatcc agaaagctgg gaacttttca
     5521 ggggtagata agttagcagg ggcgcaattc cagatcaaaa attcagagaa tcaattcgtg
     5581 accggttttg atgaaaacac tggtgtcgct ctctatgatc ctgctgagtc agcgttgaca
     5641 ttcacaactg atactgaagg atccatcagt gtcaatggat taatagacgg caattatact
     5701 ttagttgaaa caaaagcacc agatggttat caattaacca atccacaaaa agaaactact
     5761 tttatagtaa aaaacggaaa attgcaacaa acaaccggta tcatccatgg cgcagatgaa
     5821 gccaatcttg ggaatccaaa cggtgtagtc aatcgggaaa atacaactac ggataacacg
     5881 attactgtag aaaatgtcca aaactacggt gatttccact tcatcaaaca agatgtgaac
     5941 acgcaagaaa aattagccga tgcagaattt tctattctaa gagcaaaaga tccaactgtt
     6001 aaattaatga aaaaaatagc aaaccttgcc tcaggagaat acccatatgc ttggtcagat
     6061 gaagtcacag gttctgactg ggaagaagtg aaattgattt ctgattcaga agggaaattt
     6121 tcaatcgaag ggctgaaaca aggggcatac tgccttcaag aaacaaaagc accagaaggc
     6181 tacgcattac ctaagaatga agctgcgtac actgaattta cagtagatga tgatccaaca
     6241 acttctgatg aaacaacgat cgataaccaa cctaaaggaa tcttgccttc aactggtggt
     6301 atggggatca tcgtattcat cttggtaggt actgctttag ttggaggagc agtgatctat
     6361 ttcaaaaaac gccatcaaga aacagaagct taaactattg gaaacaaaaa tcatacgata
     6421 aataaacgat agtgattcaa atggggagaa aggttgtgac aagtggtcgg tctcttggta
     6481 ttcgctcaac aaacggcaaa ataggaggaa ctattttgcc gtttgcttcg ctaatactca
     6541 ggtcaaggcg ctgatttcac aacctcttca tatacatatt agaaaatagg tggacatgat
     6601 gaataaaagc agaataagaa tactggacat cttcatggca atcattttag tcgttgggat
     6661 cgggatcttt tcttatcctt ttgtagaaga ttcactgaat gattttcttg cccagcaaat
     6721 gatcatccat tatcaaaagc aagcatcgaa taagaattca gaggaaataa aaaaacagca
     6781 ggaaaaaatg acaaagaaaa accaacagtt ggcagagcaa aacgtatcac cagggatagg
     6841 cagtttcaac caagcagttg atgcaaaagc attaaaagat ttacctagta atgccttttt
     6901 tatggaacat atgcttggag tcatcgaaat cccaaaaatc aatgtcagct tacctatatt
     6961 cgatcagaca acggaaatat ttttgcaaaa aggaacgtca ttattggaag gaagttctta
     7021 tccgactggc gggaaaaaca cccatgccgt tctttctggg catcgtggac tcccagaagc
     7081 aaaattattt acagatttgc ctaagttgaa aaaaggagac cagtttttca tccagatcaa
     7141 tggaaagaca ttggcgtatc aagtagaaaa aatccaagtg gtcttacctg acgaagtaga
     7201 ttctttgggt atccaaaaag gcagagatct cgtgactttg cttacctgca ctccttatat
     7261 ggtcaataca catcgactgc ttgtgacggg gcatcgtatt ccttatcaag caaaagaggc
     7321 aaaaaaagcg atccaaggaa ttgatcaatg gaaaaaatgg aaatttttta tttggtttat
     7381 cggtatacta cttggatcaa tcggcttagt ttggctactc atcgcttatt tagattcttt
     7441 ggccatcgct aagcgaaact atcctttatc tttttatgta aagaacacaa acggacgacc
     7501 aatcgaagga atggtctttt ctgtaaaaac gctcaacggc aaacactata ttactagaga
     7561 aaaagttcca tttgtgaaag cttctgatga atacggacta gtgagatttt ctgatttaaa
     7621 aggcaggaac tatcgcttgc aacatgagga gctcctgctt aaaatccatg taaaacataa
     7681 acattcgaaa cagttttcaa tgaaattgaa aaaaggaaga tataaactgc gaaaagaaaa
     7741 agaggtgtac tatttgattg agaaagaatg aatgaagatg tccaaaaaag cagagggaac
     7801 agactaaatg aaaaaagaga gaagctgacg aaatggacgt gtaaactgct atttttggtt
     7861 tactatctgg aacgccagct tctatttttt tatgtttcag aagaatttta ttattatagt
     7921 ataacttttg aattagtttc cataacattt ggagcaaggg gttaaaccac gagcttgagc
     7981 ttcgcttaaa gtggcagggg tatacgtacc atttccacat ttatgtgtat ggtattttga
     8041 accggtcggt gtgacataga cagtttgttc attattttgc tcaggagctg cggcttgtgc
     8101 ctgctgagaa gcctgttcag ctgctgcttg ctgtcgcgcc tgttctgctt gttgctgtgc
     8161 gattgcttct tgcctcgcct gttctgcttg ttgctgtgcg attgcttctt gttgcgcttg
     8221 ttctgcttgt tgtcgtgcaa tttcttcttg ctttttccgt tcttcttctg cttcttttgc
     8281 tttgattgtg gaatcaattg cagcaagacg gcttgtcaac tcttgtttgt tttcaggtag
     8341 cgcttggata gctgctgaag cagttgcata gttttctttt gtaggatttg cttcagcttg
     8401 tgacaaagca gattccgctt ctgtcacttt tttttctagt gcagcttttt ccgctttttg
     8461 tttctcttct tcttttttct tttcaatttg ttctttcgat gaatctgccg tactttcgga
     8521 gatcgtggta ctgctgctgg aagcctgagc tgaacttgtg ctttgagtag tagacgtagt
     8581 agcaccacac ccactcaaca aacttcctgc gaccaaaaac aacgcaacta attttttcac
     8641 taaatttcct ccttaagtaa aaaacaaccg ttttcaataa atgtcctttt caattgttta
     8701 cttacttgtt ataataattg tgttatgaat agtgattgag gaaacttaat ctaatcccgt
     8761 gttgatacac gggatttttt gctatagttc tgttattaag tatgcaataa taaaatatgt
     8821 acgttatttt attattagat tattgacatt tatataataa acgattaaat gattcatatc
     8881 aatagaatac ttaggaaaaa aagagaaata ataacaggtt tatgcactat tgacggtgtg
     8941 acaatatgat atttgtatcc gtttttttct tgtatgtagt atagtaaagt agatacagga
     9001 ggagaaaata tgcagacttt tttaacaaaa gtttctcaaa agaaaattaa aatgattcat
     9061 ttactgcttg aaacagaacg ttggtgttca atagaagaac tacaaaacga attgaaggta
     9121 tcaagtaagt ctatcttgaa ttatctaaca gatttagaag aattgttcca acaatatcct
     9181 gataaggtca ttttgaagaa tgagaataat cggcgctttt ctatggaaaa gcaggagaac
     9241 ttcccaatct acattattta tcttcatttc tacagaaaat catataatta tcatttgatt
     9301 gaattcatgt atcaacatcc tgagaaaaat ttggaagatt atgcagacgc tcaatatacg
     9361 agtgtatcca cagtttttcg ttatgctaag ttattagtaa agtactttga aatgacggac
     9421 gtcaaagaat agtcgccgcc agatagaagg aaattagcag ttgaaaaagc tgccctgcat
     9481 caagtaatac gaccagccaa acggaaacag tgtctcatgt ttttgatttg gctggtctta
     9541 ttaccgcaga tgcaagcttt ttccacacct ctttcttgtc atttacagac tcaactatca
     9601 atgctgcaag ctttccctta atgctgtaac accagtaaga gttgtcaaga ccagcagact
     9661 gactaggtaa ataccgctca aaaacaagat tacggcagtt aaactataat gatccatcaa
     9721 gaaaccgatg acgatagaag aaaaaccacc aacagctcgt ccaaggttca taacgaaatt
     9781 attcgccgtc gcgcggattt ctgttggata caaactacta atcactgcgc catatccacc
     9841 atacatgcca ttgataaagt agccgactat cacagcggct gctaccaaac tccactggct
     9901 atgggctaac acaagtagat agacagataa ggatgaacaa attaaaaaca aagaaaaggc
     9961 tagtttaggc cccaatttat ccatgattat gccaaaagtc atcatcccca aagacatgcc
    10021 taaaatagta ctgaccatcc acaatgaaga cccagaaacc gaaagcccta atcgattttg
    10081 cataattgaa ggtagccaat tcattaaccc aaaatagccg gcaatttgga cagttaccat
    10141 gatactaagg ccacatgttt gccaagcaag atgttttgat tcaaacagtc tgctaagttt
    10201 ggttttcttt tcggcagatt gtacagattt gaattccttt ggttcagtca agccgcgacg
    10261 aatgaacaag accatcaaca ccggcagtac acctacgaca tacaacatct tccaaccaaa
    10321 cgttggaatg atcacagcag ctaaaatcgc agccaaagcc gcaccgacct gcgcaccaat
    10381 accagcaacc gaagaagctc gtccaatttg tttttttgaa aaactttcgg aaatcaagga
    10441 catacacgcc ccatattctc ctcccgctcc tacacctgcg ataaagcgaa agagataaac
    10501 caagtagatg tttgaagcaa aatacatcaa taaagaagca atcgaaaaca gaaggaccgt
    10561 ttgagaaaat actttcacac gtccatattt atcagctatg atgccaaaaa aaatacctcc
    10621 aatgagcatc cccaaattag tgatcgttga aatgaaaccc gcttgcgtac cactaatatg
    10681 aaaagaagta atcattgaag ataatgaaaa agctaaaaac ataatatcca tattctctaa
    10741 agcaattccc gccgatgtcg atactaatac ctttcgctga tacgttgtca tttttatacc
    10801 ccttttccta aaatttccct aaattgttaa aagtatatcc aaaaaagaat aaaaaaacaa
    10861 gactttttta tttgaaataa tacacaaaca accgatatag ataatccagc ttagttttaa
    10921 tagaaatgag tattgacttg gagtacactt aaagttgtag aataaataaa atacataagt
    10981 aaagtggcaa gaaattaaga gaaagagtga tcagatgaat atcaaacaag tcagtgaaga
    11041 aaagggcatt tcggccgata cattgcgcta ttatgaacga atcgggttga ttccaccagt
    11101 taatcggaca aaaggcggaa tccgtgatta tacagaagaa gacctaaaat gggtcgactt
    11161 tacgatctgt atgcgaaatg ccggattatc gatcgagtct ttgattgaat atattcgcct
    11221 atctagcgaa ggagaagcaa ctgtatttga gcggcgtaag cttttgattg aagaaagtga
    11281 acttttgacg aaaaaaattg ctgaaatgca agaatgtttg gaacgtttac aaggaaaaat
    11341 caagaaatat gatcgtgtgt tatctagaaa taaaatggaa tgtatcaagt aaaaaaatca
    11401 aggagcatcg ctatggagtt acgcttacta aaatattttt ggacaattgc agaagaagga
    11461 acaatctctc aagccgcaga agttctacat cttacacaac caaccttaag ccgacaactg
    11521 aaggagttag aagagtaact aggaacagaa ctgtttgtac gcgaaaaccg caaaatgatc
    11581 ttgacagaag caggttattt tctaaaaaag ccgagcagaa gaaattttag atttaacgaa
    11641 tcaaaccact caagaattcg agaacagaaa aaaacaatta ttcagcggcc gatttacgat
    11701 cggctgtgtg gaagctgata gttcgaatac attggcacta ttactagaaa agttgatccg
    11761 tgattatccc agcgttcaat ttcatatcca cagcggaaca agtgatgata tcacggagtg
    11821 actagaaaaa agcctgttag atttagcgat tttgctagat ccagtagata cagagaaata
    11881 cgaaacgatt ttgttaccaa gaaaggaaaa atggggattg ctcattttag aaaattctgt
    11941 tttagcaaaa aaagacgtga taacaaaaga agactttgcg aatgtacctc ttttgatctc
    12001 aaagcgtcca gaaatacagc gaatgtttgc tgactggtta ggcataaaaa aggagcagtt
    12061 gctgatcaca ggaacgtaca atttgatttt caatgtattt gcgctaattg agaatcaggt
    12121 aggtgctgct ttagtcatag aaggtgctgt aagtcatcat tcaaagcgag tgacattcgt
    12181 tccgcttgac ccaatgattg aaacgaactg catgctagtc tggaaaaaga atcggatttt
    12241 atcacctgta gtcaatgaat tgattcaccg tttcaaagaa gcttttgaac agtaataagt
    12301 atctctaatc gaagaaattt caatacaaaa gcgagacgta tgacagtctt gcttttgtat
    12361 tttttcattt ttaaatcaaa aaaataaaga gatttacata acttgttaaa taagcgctta
    12421 caaaaataag aagaacggaa atgaatcagc tataataacc gttgttctgg attgtaatca
    12481 ttacaaagaa atgaagatta taactttgga ggtgttcgaa atgaaagaaa aaaagcaaaa
    12541 attcgttcaa aaaacatgcc ctttattcaa tggttgggta tgattctgct gattgtatta
    12601 gctttattga cgatcattcc ttttttatgg atggtttctg cttcattcaa agcaaataat
    12661 gaagtgttca cgattccgat tcaatggatc cctaaaactt ggcatttaga gaattatctg
    12721 gttatttggt ctcgtattcc tttagtgaca ttttttaaga atacagcttt tttaagcgtt
    12781 gtgatcacgt tgattcagtt gtttacttct agttttgctg cttacgggtt ttctaaacta
    12841 aattttaaag ggcgcgatat tttattttta gcatatatcg gcacgattgc tgtcccttgg
    12901 cagtcttata tgatcccgca gttcattatg atgagaaaag tgggtttaac agatacgttg
    12961 tggtcgttgg tgctattaca agcgttcaat gcttttggtg tgttcttgtt gaagcaatat
    13021 tacagtagta tcccagattc tttatgtgag agtgcgcgga tcgatggctt atcagagtgg
    13081 ggaatctatc gcaagatcat tttacctctt acaaaaccgg cgattgccag tttgacgatc
    13141 atcacattcg ttaatacatg gaacgattat atggggccat tcatttattt gtcttctacg
    13201 gaaaataaaa caatccagtt agggttgaaa atgtttattg ggctatttga tgcagagtac
    13261 gcattaatca tggctgcctc agttgtttct gttcttcctg ttgcttttat atttttagcg
    13321 atgcaaaaat attttgtaga agggatcgct tcatcaggaa tgaaggaata ggaggtgatt
    13381 atgaaagagc agcgttttga caaaaatgtt tatatggaat tcttttattg gttgtatcaa
    13441 tcattggcaa gtagcctttg tttttggtgt gtcaatatct tgtatatagc agctgcgttc
    13501 cttttgtcga tagatggaag gaatgtcttt tcctttattt tttctttact ttttattggt
    13561 ccaggttgca tcgtgatgat aggtatgaca gaagaattag caaaagaaaa tagtttttca
    13621 ccagtacgtt tgttcataaa gatgtttcgc caattttggc taaagggatt tttttattgg
    13681 ctatttgctt ggatagttag tgtgataatg atttttgatt gtttcttttt tatacgtttt
    13741 tcttatggaa aatggttgat tccgttgttt gttttacttg cttgtttatc agtttccttt
    13801 agcaccaact gctggtattt tcaggtgaga aatccagcgt cgaaaccaaa tcaagttcta
    13861 cggatagcgt tctactatac gttgaaaaaa tggtatgtga gtttactaga ttttttacta
    13921 ttgacaagtt tgtttctctt ctttttcgta aagccccaat ggtgtattct actaggtcct
    13981 tctatcgtat ttggtttgat ttacttcaac aatcgtaaat tgatgagaac tatggatctg
    14041 tagactttat aaattgaaac tttcaattaa gaatgacagt tttttttatg taaaaactgt
    14101 cattcttttt cattttttga aacatatggc taaacaaatg aaataaaacg tgctatactt
    14161 ttgtatgaaa caggtttaga aactgtatct cttatttgtg attatttcta actaataatt
    14221 aaaaataaag cataaaaagg acgatgttca atggctgaaa aaatgaaaca aggttcgaaa
    14281 tctgtagtag aaagtttgat taatcatcat gttgattatg tattcggtat accaggtgca
    14341 aaaatcgatg gtgtatttaa tgaattagag gatcaaggtc ctgaattaat cgtgactcgt
    14401 cacgaacaaa atgctgcatt tatggcacaa gcaatcggac gtattacagg agagccagga
    14461 gtagttatcg caacaagtgg tcctggtgct agtaacttag ctacaggttt ggtaacagcg
    14521 acagcagaag gtgacccagt tttagcaatt gccggacaag taaaacgaag cgatctattg
    14581 aagttgaccc atcaaagcat ggataatgca gcactgttcc agccaatcac aaaatacagt
    14641 gcagaaatcc aagatccaga aacaatttca gaagtgattg ccaatgctta tcgtatggct
    14701 aagagttcaa aaaaaggcgc gagctttatc agtatcccgc aagatgtagt ggatgcacct
    14761 gtccaaggta atgtcatcaa gccattgagc gatcctaaat taggttctgc ttctgcagat
    14821 gatatccgtt acctagctga acgcattaga gaagcaaaac taccggtact attagtaggt
    14881 atgcgaggat caagtgaaaa agaaacgtta gccatcaggc aattagttga aaagacagca
    14941 ttgcctgttg tagagacatt ccaagcagct ggagtgatct cacgtgaatt agaagcgcat
    15001 ttctttggtc gtgtgggttt atttagaaac caaccaggag atatgctttt gaaacgaagt
    15061 gatctagtta ttgcaattgg ctatgatcca atcgaatatg aagcaagaaa ctggaatgcg
    15121 gaaaaagatg cgcggatcat tgtgatcgat gaagcgccag ctgaaatcga tccttttatg
    15181 cagcctgaaa gagaattgat cggtgatatc tcagccactc ttgatttatt gacaggttca
    15241 ttagagcctc aacaagtgtc agaagatgca aaagaatatt tagcatctct tcaagcaaaa
    15301 ttaacggagc gggacattgt tcaatcaaaa ggagaagcag gtatcttgca tcctttagaa
    15361 gttattaaca cgcttcagtc aaaagtaaca gatgatatga ctgttaccgt agatgtaggt
    15421 agtcattata tttggatggc acgccacttt agaagttatg aacctcgtca tttgttattc
    15481 agtaatggta tgcaaacgct tggtgttgca ttgccatggg cgatttctgc tgcattggtg
    15541 cgtccaaata cacaaatcgt atcagtttcc ggcgatgggg gatttttatt ctctgcccag
    15601 gatttagaaa cggcggtacg taaaaaattg aatatcgttc atttgatttg gaatgatgga
    15661 cactacaata tggtagagtt ccaagaaaaa atgaaatatc agcgggcgtc aggtgtagac
    15721 tttggtccag ttgattttgt gaaatatgca gaagcttttg gcgctaaagg aatccgagct
    15781 acaagtgtag aggaattaga aaaagcttta gaagaaggat ttgcgacaga aggacccgta
    15841 attattgata ttccgattga ttaccgtgat aatgagaaat tgggagaaac aatcttacca
    15901 gatcaattct attaaggaag tgtcgacatg actgaaaaaa tcttatacca gcacgggaca
    15961 ttaggagctt tgatggccgg cttgatggat ggaacagaaa cgattgctca tatattagaa
    16021 aaaggaactc tgggattggg aacattgcat gggcttgatg gtgaagtcat ctttttagat
    16081 ggcgtagctt atcaaggaag atcagatggc tcagttgccc aattagatgg aagtgaactt
    16141 accccctatg cagcaattac agattttacg ccagatacta gtttttctgt ctcaaaaact
    16201 gcagatggag aacaattgaa aaaagacata ttagtaagag aagccgggga aaacctattt
    16261 ttggcagtga agataactgg attattcaaa aatatgcaca tccgtatcat gccgaagcaa
    16321 gaaaaacctt acagaagact agcaaaaatt tcagagtctc aaccagaatt ccaacaaagc
    16381 aatatccaag gaacattagt cgggttcttt acgcctgaat tattccaagg agttgctgca
    16441 gcgggatttc acctgcattt tatcgatgat acacatacat ttggcggaca tgtaatggat
    16501 tttgaagtgg ctgaaggaaa agttgagatt agccgcatct cgtcccttgt acagcatttt
    16561 ccagttgatg atccgacttt tgttcacgaa acgattgatt atgctgattt agcagcagaa
    16621 atccaacaag cagaatagaa aaagacagcg agatgcgttc aaggtgatta acctgacgta
    16681 tctcgttttt ttgaggagga aacctagcgt ttacaaaaaa actgagctat aatcttctcc
    16741 atagatcaaa aaggagcggt ttttatcatg tggacagaaa aacagaaaaa agatgcatac
    16801 gaaattttat tattacagca acaaggatta ttagaagcag aagataactg gttagcaaat
    16861 ttagcaaatt catctgcatt gttgaatgag actttgcctg agactgtctt tgcaggatat
    16921 tatttattcg atggtaatga attgatttta ggaccgttcc aagggcgagt ttcttgcaca
    16981 cgtatcaaga tgggaaaagg agtttgtgga gaatcagctg aaaaacaaga aacattgatc
    17041 gttgatgatg tcaaaaccca tgcaaattac atttcctgcg attcagccgc acaatcagaa
    17101 atcgtagtgc caatggtaaa agaaggtaat ttaatcggtg ttttagatat cgattgcgga
    17161 gtaacgaaag ggtatgatga aatcgatcaa gcatttctag aaaaatatgt agatctttta
    17221 ttagcaaatt ttaatataaa tgctttttca tttattaata actaaaatcg tctattgaaa
    17281 caaaaaagaa gagaaatcaa gaataaaaag agccggaaag acagtggtca tttctgtttt
    17341 tcggtttttt tattttttta taaaatcaca agtgttattg attttcacaa tcatatttta
    17401 atggtattat cattttgaat aatgaaaaaa cattaaggat ggatgaaata tgaataacga
    17461 aactatcatt ttaggaaaaa aatatatttg ccaacctatt ggcttgaaac aaaaagtggt
    17521 tggagaagta atcagtaaat tagaaaattg tgttgtgatc tgcgtagacg aatatcatga
    17581 cgtcgatcgc aatgaaatat tagaaaaatg cggaaaagta gtggcaaaat atactcacgt
    17641 ttatgagttg gaaacaacag aatatctata taactatagc aacaaatatg aacatacttt
    17701 agtttcataa agatttaaat aaagtgcctt caaaagcgag aagaccaagc ttttgaaggc
    17761 actttattta gttggcagtg attgttcatg atttttgatt tattcaaaag gttcgcctag
    17821 agaatctgtc tctgtaacga ggcctggtgg agaaactcga taaaaaccta tactgccaga
    17881 actgtcctat tttcggatag acaaactagt agttttaaaa agatcatccg atccaatcat
    17941 ttcaaaaaat gcaaaactga tatcaggatc atttagctca ggcttgttat cgtaaagcat
    18001 ttgtaaaggt tgcatttagt taggagtaaa cgcgaaacta tcatcattat ttgctgaaga
    18061 agatgaagaa gctaattcag agtttgtttg aaataaagga gacgtgctga cagtttcttt
    18121 accctgtttt ttgcagaggt agaaactttt atatcggaag tttctaccgg agaatttttc
    18181 tcatttgtag aaattgttga atcgatacta ttcgaaattt gcgtatctga aggtatgttc
    18241 tttgatgcag aagtcccaca agcagtaagt aacaataaat ttgataaact gataaacaga
    18301 attcttttca ttatgtacac actccttata gtatcgttta gcatacacct atgctcagta
    18361 ttaggcaaaa tcaatgcaaa actatcaagt cttttatagt atctattagt cttagtaata
    18421 catatatgga gagggtgata tcccagttca aaccagatac attggttacc atctgtatta
    18481 acgattatgc ctctaaacgc tttttttatg aaaaaattta taagtgaaaa cgtaggtatg
    18541 atattatcta gaggtaattt ttttgggtat ttaaatagtt gctaggagga tagcttttgg
    18601 atttttacag taattttatc ttaataatag caatattatt acttctaaat atttggtttt
    18661 ttgataagag tagaaatgca gggataggtt ttagaaccaa acgaagtacg tcttctgaaa
    18721 aaaagtgggt atattcacag actatttttt acggaggggt tatttcaatc agtttacttt
    18781 cttcaacact ttattctttt aatgttatag acgtttccat gtcaaatttt atatctatta
    18841 ttggaatcct tatttctgca attattacac aactacttct tgtgtttgaa gagaagtcaa
    18901 aaaataatta aagcaattat gccttttagt acaggaatta ttacttctta caaatgtata
    18961 gcgaatgtga agcctgctgt tttaattgtt ctagaaaaac ttttgagtga acaattattt
    19021 tatgttagga tgaattaatt tctctagagt ttataattaa aaaacttatt tgaaggaaca
    19081 tgcttaaacc ataattgtgt ttttccttcc atcagcataa aagttcacct caaaaagagt
    19141 gtacgaacaa aagttctttt tgtaaacctt aaaatagatt ttcctgaata cgtattgggg
    19201 atttctaaat gtttgaatgt ttttttgaat gcaaaatcaa tgcaaaaaat tttgtatttc
    19261 tctacttttc tctccttgac caaaataaaa aaacgttgat ttaacaacgt ttttcataca
    19321 tgtttatctg tctcaaccat acatatatgg agacggcggg agtcgaaccc gcgtccaaac
    19381 acatcgccac ttaaaaatct acgttcatag tcaactcatt taaggattca ctttacagct
    19441 tgccgagtga caggcattct gtatcgctag tctgatgttc tctttttcaa cttacagacg
    19501 gaaagttgaa acgtatccca ctaaagttag gacccttact cgagcacatg ggcgatgccg
    19561 agaggatcta cgctaactgt ttttaggcag ctaaagcgta agagttgttt tcgtttttag
    19621 cagttatatt taactgtaac gtttttacgt agacgtaacc tacgaaacgc aattcaagct
    19681 cgactgtgcc tgtcgaatcc gtaacgtccc cgttagctaa caagggttac gtgtattttt
    19741 catacaaaat tagtatagca tatattggga taaattcaat aaaaaagcga atcatatccc
    19801 attatttata taataactgc ccctttctta agaaaaaata agcgattttc ttaagaaaag
    19861 aggtagcttc aaaaaaagat cttaaatcag atcaaaatac ttcaatgcgt ggacaattcc
    19921 gccttctgta tttttctttg tgataaaggt agaaagttct tttaatccgt cataggcgtt
    19981 gcccatagcg attttgttgt cgcaagcttc caacaaggca aaatcattcg gaccatcgcc
    20041 aaatccaaaa gtaggaactt tttccagctg aagttctttt tgcagacgtt tgacacctgc
    20101 cccttttgag attcctttct tcactgtatc gatcgagtaa ggaccattcc gataaaaagt
    20161 tagctcagga aaatgttcgt gatagtattc gtcacctgat tgagataaga tcagcaacat
    20221 atttactgtg ttttctttaa agcctaaagg atcaatttcc ggaacatctg aatgaatgaa
    20281 gtcgtaagca ttgatcattg tttggctatg accggtacac cagattttct gatcgttata
    20341 gtaagaaagt tcatgaccgt tcaactgtac atgatcatac attttttgac attcttctga
    20401 agaaaatgtt tgagaataga tttcttcgcc gtctactcga atgaaagaac cattcatggt
    20461 gaccgcagaa gtaatgcccg catcttccat gatagagtgg atttctgctt ctgtacgtcc
    20521 tgtagcgatg acggggagaa cattgttttc ttttaatgcg ttcatagctt ttgccacttc
    20581 gggtgtgatt tttgatttcg catctagtaa tgttccatca agatcaaaga aagtgattgc
    20641 acgataattt gacacaaata aaactccttt tttattggaa taagtttatc ttaacaaaga
    20701 atgaacaaaa gacaaacctt tcgtgaagaa ttcctgaaca tattggttca gtcaacaatt
    20761 tagagtatac tataagaaga gaatttagga gggaaagaat gatttttttc agtattggta
    20821 tcttattctt aataattggc agtctttttc ggattctgcc ctcaaaagga aatctgcctt
    20881 tttatgggta tcattcgcct cttgccgcaa aaacagacgc tcattggcgg ctggctcaaa
    20941 agacaagcgg aaactggttt tttcttatgg gcttactgat ggcattgatc ggctattatt
    21001 taaagacatc tgggcatacg aattattttc tgattgaaat gcttctgcta gtatttccaa
    21061 ttatgcctat ttttattatg acagaaaaga aattacagaa atatgacctg gaaactggag
    21121 ggaacgataa tgaatatttt aatgattgaa gataatcaat ctgtttctga aatgatgcag
    21181 atgtttttct taaatgaagg ttgggaagca actttcaagt atgatggaaa agaagggctc
    21241 gatgcctttt tagaaaaccc acaacattgg gatatgatta cactagacct gaatttgccc
    21301 acactggacg gtatgctagt aggaagagag atccgcaaag tttcaagtac tgtgccgatt
    21361 atcatgctga cagctagaga ttctgaaagc gatcaggtga tcggcttaga gatgggggca
    21421 gatgattatg tcacaaaacc tttcagtcct ctgacgctta ttgctcgaat gaaagctttg
    21481 catcgccgta gtgagttggc agaagcaaga gaagaagcag cagtttcaga tgatcatttt
    21541 gatatcgaga ctagtcattt caagatgaat acgaaaacac gagaagcgta tctggacaac
    21601 cggccaattg aaggactgac gcctaaagag ttcgatctat tgtttacttt agcaaaaaaa
    21661 ccacgtcaag tattctctcg ggagcagttg cttgaattag tctgggatta tcagtacttt
    21721 ggtgatgaac ggacagtgga cgcccatatc aagaaattac gacaaaaaat cgaaaaggtc
    21781 ggaccacaag tcatccaaac tgtttgggga gtcggctaca aattcgatga ttctggtgtt
    21841 gcctaatgcg ttatttgtat cagcaactct tagctttttg cggaatggtc gcgttgatct
    21901 tattgatcgt tggagtttct tttacacaac tgacaaaaca aacgattgaa gaaaacaatt
    21961 accagcaact gtttggatat gctgaatcag ttgaaaagac aacgcaaacc tatgccgata
    22021 cgctcccgca gtttagtcaa gatcaggcgt ttcaaaatgc attgctgctg acagagcaag
    22081 tactgacaga acaaaatgtg aactttatat ttatcgataa aaacgaacgt gtcatttatc
    22141 cgactaatac agctttactg aattttacct taacagctga tcaatggaaa aatctgagga
    22201 atggacgtca agaaaaattc acatcgaata aaaatatttt aggacaagca caggcgacct
    22261 cttacgcact tgtgccattt aatttgaatc atgaattcta tggagcactt gtagttagtc
    22321 aacctgcgcg aaatatcgat aacagcgttc gtccaattac actgaacctg ttcaaaggat
    22381 ttattttttc cagtatcgtt gcagttattg ccagctatgc ttttgcagca ttccaagtta
    22441 agcgtatcaa tcggttaagg aatgcgacga aagaagtgac gaatggtaat tttgatgtac
    22501 agctccccgt acatgacaaa gatgaatttg atgagttagc agatgatttc aataaaatga
    22561 cgaattcatt gaaagaatct caagcagaaa ttgaagaaca agaaaatcgt cgaagacaat
    22621 ttatggcaga tgcttctcac gagatgagaa cacctcttac gacaatcaac ggacttctgg
    22681 aagggttaga atacaatgca atccctgaaa atcagcgaga aaacgcgatt aaattgatga
    22741 aaaatgaaac agagcgtctg atccgtttgg tgaatgaaaa cttggattat gaaaaaatcc
    22801 gtacgaatca aatcagtatg gtcattaaaa aattcaatgg aacagaaaca ttgaaaaata
    22861 ttgttgcaca gttagaagct aaagcagaag cagcaggaga tacattgaca ttgaaagccg
    22921 ttgatgatat tgatgtttac gctgattatg accgatttgt ccaaatcatg gtcaatatta
    22981 ttcaaaacgc gatacagttt actgaaaacg gccaaatcac gattacttta gaaaaaggct
    23041 atcttgaaac gatcatcacg attgaagata cgggaattgg aatgtctgaa caacaaatga
    23101 aaagtatctg ggatcgttat tataaagtag atccttcaag aaaaaataca aaatatggtg
    23161 aatcaggatt aggtttacca atcgtgcagc aacttgtacg cttgcataaa ggaaaattag
    23221 aagtagaaag cgaactagga aaaggaacaa aattcgctgt tcgtttgcct gacgtagaaa
    23281 tcaaaaaaga gtaaaaaaca agcggtataa aagatcatga atcgatcttc tataccgctt
    23341 gtttttacta taaaatgatt gatttagccc gcgggctcta aacgatcaat ataaggactt
    23401 tgagcaatag tagcatcatg agtaaagagt aagatacctt gattttcttc atgagctaaa
    23461 taagaaagca agtggagtag ctcttggatc tccaatacac taagttcatc aaatgtatct
    23521 tcaataacaa tattgtttcg cttggaaagc aaggcatgaa gtaattgcag ctttactttt
    23581 tcaaaagacg tcaactcagc tgccgcacaa ttaagcaagg aaggagagat atcaagtttt
    23641 tcttgatagg ccaaacgatc ttgtttgatt tctttttccg gtacatccaa caggacatgg
    23701 tctttaagat tcagataagg aaacatcggc caattttttt ggatgatcgc aaaattatcc
    23761 agttttgcaa caagaacctc ttttttcaag tgctccaata ttttatgctg tttttcgaga
    23821 ggacacaaaa taacggtcaa gtgttcagca tgaatcaaat attcaggttt cattacgtcc
    23881 aggcctccta ctttgcaaat gatggataaa aaaagtgaaa gggaatagac taagaatgag
    23941 acagcatcca ctgagcccaa taagagtttg ggcaaatccc ttaagtatat ctgtgaaaaa
    24001 aagatcattt tggaaatcga tggtaaaaaa agtctgttga tccatcggaa ttgtaagtcc
    24061 gcttaccgaa gtcaagatat cacggatatc tggcagttca gtagtcttta agacaagaaa
    24121 attgatcctt tgaagaagcg cttcataaac tgactggaag cttacgatcc acaataaaac
    24181 caagatacaa caaagaaaga gaggaaagaa cagctgccag aaaataaaaa ggaataattt
    24241 ggttttagaa agaccagtca ggcgccaatt gatcaattcc tgcttgtttt gcctgactga
    24301 atagtaaaag aaaaagataa aaaggaaggc aaatagaaaa aataaactga atatgccttt
    24361 tatataaaat cggctagcta attctaaagc cggaagaagc tctgtagatg cagaagcgcc
    24421 aataaatgaa agccgatctt ggacttgatt ggctaaagcc gattgcaaat ggtaaagagt
    24481 caggcatgct atcaataaaa aagcagaaaa gcttgaaaat aaacaataga gtattgtctg
    24541 ctttttgtaa taaattaatc gagcagtagc atagcggatt attttcatac ttttcacctc
    24601 tataaaaagt atagaaaatg aatatgaata aagtatgaaa ttcctttagt gaaatcacga
    24661 attaaaacca gaaaatattt tgtaaaaagc aagaggttgt gaagaagctg ccctattcta
    24721 ttaccgtaga tgcaagcttt tgaacactgt ttattcgaaa agaggagcag cgacaataat
    24781 tttgtcgctg ctccttttaa atttattttg tcattcggaa gaacttgtgg tcgttccgtg
    24841 aagttcttgg tacgtttgcg ttttataaag atcaacagtt gattgtccgt tgtgttcact
    24901 aaagacacta gttgacttgt caccaagaga tttttcagct tcttttaatt tctctaagcc
    24961 attcttgtag tcataatcac tggtatcaat tgggtctaat ccactattcg tatagaaccg
    25021 aagaagatca ccattattga tttggtcaga aacagctaac tgtttatcaa ctttttcttt
    25081 cagtgaatca atttcattct gtacttcctc agtaggttca gtaatagctt caccagtttc
    25141 gttactatag atcgtaccac cgtagtaagt atattttggt gtaacaaaat caccatcacg
    25201 gaaagaaaca agctgttcat gatctttcga gaatagatct tgtcctaact gaatataatt
    25261 ttttgtatct acacctagta agtgaagtaa tgtaggcaag gcatcaactt gtccgccgta
    25321 agtgtgattg attccgccct gtgtttgtcc agggatatgg atcatataag ggacacgctg
    25381 catttgtgca ttgtcgtagt ttgtccaagt agaacttgtt tttccaacta attcagctag
    25441 atttttattt cttgaattcg agacaccgta atggtcaccg taaaggacga tgactgaatt
    25501 atcatacaag ccgctttctt tcaaataatt gaagaattct tgtacggcgg tatctaaata
    25561 attggctgtc gcaaagtaac cgttgatcgt ttcatcattt gtattggcta acgggaatcc
    25621 tgcttcgtca ttggtgaatt ttgaataagg ataatggtta gatacagcaa tgaattttga
    25681 ataaaatggt tgttgcagac gttccaagta ttgtacggat tgttggaaaa atggtttatc
    25741 atgcaacccg tattggaacg aattgtcatc attaacatca taataagaag aatcaaagaa
    25801 ataatcatat cctaaattct tgtatgtctc gtttcgattc cagaatgtac cagcgttccc
    25861 atggaatacc gcactagtgt aaccttgtgt ttgacttaaa atatcaggtg cagcttggaa
    25921 agtatttttt ccgcctaatt gtgtgaacaa ggagccttga ttcaagccaa acaatgaatt
    25981 ttccatcagc gtttccgcat cgcttgtttt tcctgctttt acttgatgga aaaagttgtc
    26041 aaaactgaac gtgctgttgc tgtggtaaag actattgatg aaaggtgtaa cttcatgctg
    26101 gagaccattc tcatctgtca atttataatc gatcaggaat tgctggaagc tttctaaatg
    26161 gatataaatc acattttttc ctttagcgag cccgaacaaa tcgtcatttg gtgctgcata
    26221 atgttctttt acataatttt ctacttcttt catatcattg ggacttgctt ctgcccgcac
    26281 ttggtttgtc tggtaagttt gaacaccgtc ataaaccatg aaagcattca agccaagata
    26341 tttgaccaaa taatcacgag agaatgttcg agtcagcaat tctggacgat cagcttccgc
    26401 cataaaaaga tttcctgaga aaatcataac tgctaatgta gagatcgcaa aagccattct
    26461 tgctcggatc gggcgagggt ccaacttgat tttttttgca atcagcagaa cagtcaagat
    26521 aataaaatcg atccaataga tcacatcata tgggcgaaac agtcgaatgg cactttctcc
    26581 cagaccactc gctactttcc cagcacctaa aatcgtgttg atcgtaatga agtccgtgaa
    26641 ttctcgataa taagtgacat tcgaaaaaac aagcaatgtc aaaagaaaat aaatgaccat
    26701 catcgtgata taagcaactc tagggcgtct gatataaaga gcaattccaa gtaataacaa
    26761 tgtagttgca atagggttga taaacaagat aaagtattga atgccacttt cgattccgag
    26821 atggaaatca actaaatagg caaaaatcgt tttcaaccaa agtaaaacga ctagtaaaga
    26881 aaaaaatccg agcctcttat tcaaaaaggc aggtgttttt atacatttca agataatctt
    26941 cctttctgca cttcttaatc acagaagtgt catgaaacca tgttacacag ctcacttatt
    27001 ctcatttatt ttactagtat gacaatatag tgtcaattga aaccagaagt ttatgctgtt
    27061 tcattaaggt tttctttaag cttcatgaaa aaatgagcag taagacaagt cctatgtttt
    27121 ttgttatact tgggaagaga aagctaaagc aggtgaagga atgaaagtaa aagtaaaaaa
    27181 aagagcaagc aatcgattga aaaaaggata tccactgatc caaaaagaag accttgctca
    27241 tccccaaaaa gaaaccacgc aatgggtgga attagttgat tcgcaaaacc aatttatcgc
    27301 aaaaggatat ctaggaaagc agaataaggg aatcggttgg gtattgagcc agcagaacat
    27361 cccatttgat caatcttttt ttgaagaatt ttttgtgaag tcaaaagaaa aaagaacttt
    27421 ctttttttca gatgattcta ctaccgcttt tcgtatattc aatggagaag gagacggaat
    27481 cggcggtctg attgttgatt attatgcagg ttatgctgtt ttttcttggt ataacgaaac
    27541 gttatatgca catcgtgaat tattgattca agcttttcgt gaaatctacc cggaaatcgt
    27601 aggaggctat gagaaaattc gatttgaatc agtaaatctg cctgagagcc agttcattta
    27661 cggaaaagag gcaccggaac ccttgatcgt aaaagaaaac ggagtaaaat ttgccgttta
    27721 tttaaatgaa ggattaatga ctgggatctt tttagaccaa aaagaagtac ggggatcatt
    27781 agcagaaggt ttcgcttctg ggaaaacagt tttgaacatg tttagttata ccggcgcctt
    27841 ttctgttgct gccgcaatgg gaggggcaat cgaaacaaca agtgtcgatc tagcaaaacg
    27901 cagtttaaaa aaaacacagg aacagtttga agtgaatggt ttggatgtcg atcagcaaaa
    27961 aatcattgtt atggacgtat tcgagtattt caaatacgcc aaaagaaaac aatttttata
    28021 tgacatgatt gtattggatc caccaagttt tgccagaaat aagaaaaaag tctttcgcgt
    28081 agcaaaagat tatggggaac tggtcaagga tagtctggat attttagcag aagacgggat
    28141 acttattgcg tctacaaatg ctgcgaatgt ctcgatagaa caattccaaa caatgattga
    28201 agaggaattc catgccaaaa atgtttcttt tgaacaactt gctttatatc gtctgccaaa
    28261 agatttccag acgacggata cctttttaga aggcaattat ctgaaagtgt ttgtttatca
    28321 aattaaaaat aaccattaga aggagcgatg cagaatggtt tgggtaaagg gattgaatat
    28381 aggaagcggt agaccaaaga tttgtgcgcc tcttatcggg gccgtccgag aggaagtatt
    28441 acgtgaagca gttcttgcac gtgaagcagg ggccgattta gtcgaatggc gagtcgatca
    28501 ttatcgtgaa gttttttctt ttgatgaagt agcagaaaca ttggttctta tccatgagat
    28561 gcttgatgga ctgccgttat tatttacgtt tcgtacctta gctgaaggcg gagaacaaga
    28621 catatcgatc cgtcagtatc gcgaccttta tgaacatgtg atacacacag gattagtaga
    28681 tatggtcgat atcgagttgt tcaaagtaga aagtctagga aaaagactga tagaggaaat
    28741 caagtcttta aacattcctt taattatcag tagtcatgat tttaaagaaa caccagcaga
    28801 ccctgtcttg ctttaccgat tgaatgttat ggaacatttt ggagccgata tcggaaaact
    28861 ggcagtaact cccaataatg aacgagatgt tttgcggttg atggagttga ctagacgggc
    28921 aaatgctttt gtctccatgc ctattattac gatgtccatg ggaaatcttg gtaaaatttc
    28981 tcgtctggct ggcggaacga cgggttccgt tatgacattt ggttccttaa gcagcgattc
    29041 tacttctgct ccaggacaat taccggtcaa agcattgaag caaattattc aattattaaa
    29101 tgaaccgttg tagaacaagt ttgaggtgat attttttggc aaagaaaaaa atagtaaaaa
    29161 cgaatgcaat ccgtatggtc gaacaaaaaa agattcccta tacagagcac gaatatgagt
    29221 gggacgaaag ccacctaagt gcgtcatcgg tcgctgaaca gcttccagaa agccaatctc
    29281 gtatatttaa aacactggtt gctgtgggca atgtgacggg gcctcttgtt gctgtgatac
    29341 cgggagaagc cgaattgaat ctaaaaaaat tagcaaaagt ttcagggaat aaaaaagtag
    29401 aaatgctcca tttaaaagat ttggaagcaa cgaccggata catacgtggt ggctgctcgc
    29461 ctataggtat gaaaaaacta tttcctactt atttagatca aattgcagaa agctatgaac
    29521 aaatcattgt ctcggctggc agaagagggc tacagatgga gttagcacct caagatatct
    29581 gtgcattaac gagcggacaa tttgcagata taaaacaata aatcatacgt ataaaagaaa
    29641 gaaggaggcc gggatgttgt ttgtccagtc tccttctttc ttttttatac attatgctct
    29701 ttgcccggca ttcattcctg ctacatgccc tgtagcgaaa gccgctgtga tattaaagcc
    29761 gccagtataa ccattgacat ccagtaattc tcccgcaaaa tacagaccat caatgatttt
    29821 actctccaat gttttaggat aaatttcctt tagagaaacc cctccgccag ttacaaatga
    29881 tttctcaata ggaaaagttt tgttgattga tagcttaaac tctttgcata gttgaacaaa
    29941 atcttgaatt tgtttttgag aggcttgttg tcctgtcaga tgatccattt caagccgctc
    30001 aagaaagaaa actaacaatc gctcaggcaa tataccatgc caagcattga ccaagttctt
    30061 tttggttgct ttactttttt ctgtcaatat atgaataagc tcttgtttgg tttgtgtagg
    30121 gaaacaatcc agagaaagtg tcactggttc tcccgttttc tctaattctt tattgacaaa
    30181 acttgaacaa cgcagtgcag ccggaccaga aataccgaaa tgggtgaaaa gtaaatccat
    30241 cgtatgttca gtcaatacac gacctttttt atttaaaacg cgtaaagtga tgtcttgtaa
    30301 agaaagccct tgaagtgttt tctcctgaat atacggttca tccgatatca agggggactc
    30361 tgtcgcataa agaggcgtca ccgtgtgacc cactcgcttc acgatcttgt acccatctcc
    30421 agtagaacca gtagatggat aagtccgtcc accagttgtt aagataatgg caggagcatg
    30481 gaactcttcg aattccgtgc gtataccgta aattttatgt tctttgtgga tcaatttctc
    30541 tactcgagta gagaatagaa gcgtcacatc tagttctttc agacgattga gcaatgcttc
    30601 gataatcgtt tttgatttat ttgttacagg aaacatccgt ccatgatcct cttcttttaa
    30661 atgtacgcct tgtgattcaa agaagttcat gatatcgaaa ttattccatt gcgcaaacgt
    30721 actatataaa aactttccat ttccaggaat atgtgcaatc aaatcatcaa caggacgatt
    30781 gttcgtaaca ttgcaccgac caccacctgt catcagtagt ttttttcctg cttttttgtt
    30841 tttttcgatc aagagtacgc gtgcgccttg ttcagctgca ctgatcgctg ccatcatccc
    30901 gcttgttcca gcgccgacta cgatcacatc atacagtttt tccattccct cacctcaagg
    30961 aaagtgtagc atatctgaag aaagaaaaca gaaaaaaaat gttttgagcc aaaagagaaa
    31021 atgagctaaa atgagggaag ataagcaaag gagcgtggaa cgatgacaaa cgcaacagaa
    31081 tatgttcaag caattcaaga aaagctacat caaaaggatc aagggcaaga agaattttta
    31141 caagcaatcg atgaattcat gcccacggtt atgtcttttt tggacactca tcccgaatat
    31201 atcgaaaaga atatcttaga acttttaacg gaaccagaaa gagttattca atttcgagtg
    31261 ccttggcaag atgatgaagg aaactggcga gtaaatagag ggtaccgtat ccaatacaac
    31321 tcagctatcg gcccttataa aggtggattg cgcttccatc ctagtgtcaa tctaagtatc
    31381 ttgaaatttt tggcttttga acagattttc aagaatagtt taaccggtct gccaatcggt
    31441 ggaggaaaag gcggaagtga cttcgatcct aaaggaaaat ctgataatga gatcatgcgt
    31501 ttttgtcaaa gtttcatgct ggaattgtca aaacatatcg gcccatcgat tgatgttcct
    31561 gcgggagata tcggtgtagg agctagagaa atcggctatt tgttcggcga atataaacga
    31621 ttgaaaaaat atgatactgg tgtgttgaca ggtaaacctt tggatttttg gggaagtaag
    31681 atacgtacgg aagctactgg ttatggatta gtgtattatg tcaaacatct attgaacgaa
    31741 gaaaaagatt catttaatca aaaaacagtt tttgtttccg gaagcgggaa tgtagccatt
    31801 tatgcaatcg aaaaagtgca agagctaggc gggaaagttg ttacatgttc agattctagc
    31861 gggtacatct atgatccaga aggaatagat gtgaaactat tgaaagaagt caaagaaatc
    31921 aatagacaac gtttgacgga atacgcaaac caacgtcctt ctgctattta tcatgctgga
    31981 gaatctgttt ggacattgaa agaaaaagcg gatatcgctt tgccatgtgc tactcaaaat
    32041 gaaatagacg aaagcttggc cgaaatctta gtagaaaatg ggatcaaaat cgtagcagaa
    32101 ggcgctaata tgccatgtac agtaaaagct gtttctgttc ttcatgaagc aggcgtgtgg
    32161 tattgtccag gaaaagctgc taatgctgga ggagtagcag tatctgcttt agaaatgagt
    32221 cagaacgtcc agcgattgac ttgggaaaga gagcaagtgg atgagcagct ggatcaaatc
    32281 atggaaacga tttaccatac gtgtcgccaa acagcagccg agtacggcga cgaaaaaaat
    32341 ctgcttcttg gagcaaatgt tgctggattt gaaaaagttg ccaaagcaat ggcagcagaa
    32401 ggtttagttt gaaaaatgaa actttcttgg aaatattgtc agcattttgt ttatttgctt
    32461 gaaaaataat aaaaaaaaac gtaaagtatt acctgtagag taaacctata aggaggagaa
    32521 caaatggcac acattcattt tgactattca aaagtagcac cgtttgtcaa cgaacaagag
    32581 ttagggtaca tgcaaagcca agtaacagca gcccacaacg aattacggga aggtactggc
    32641 gcaggaagcg acttccgcgg ctggatcgat ttaccaacga actatgacaa agaagaattt
    32701 gcacgtatca aagaagcagc taaaaaaatc caatctgatt ctgaagtatt agttgttatc
    32761 ggtatcggtg gttcttattt aggcgctcgt gcagcgatcg acttcttgaa tcacacattc
    32821 ttcaatttat tagatagcaa cacaagaaaa gcaccacaaa tcttctttgc cggaaattca
    32881 atcagctcaa catatattgc tgacttgatc gaagtaatcg gtgatcgcga cttttcagta
    32941 aacgttattt caaaatctgg aacaactact gaaccagcga ttgcgttccg tgtattcaaa
    33001 gagcttcttg ttaagaaata tggacaagaa gaagcaaaca aacgtatcta cgctacaaca
    33061 gataaagcaa aaggtgcagt taaagtagaa gcagatgcag aaggttggga aactttcgtg
    33121 attcctgatg acatcggcgg acgtttctct gtattaactg ctgttggttt gttaccaatc
    33181 gctgttagtg gtgcagatat cgatgcattg atgcaaggtg ccgctgatgc aagcaaagca
    33241 tactcaagcg acaaactaga agaaaacgaa gcttaccaat acgcagcaat gcgcaacatt
    33301 ctttaccgta aaggcaaagt aactgagttg ttgatcaact atgaaccagg aatgcaatac
    33361 ttctcagaat ggtggaaaca attgtatggt gaatctgaag gaaaagacca aaaaggcatc
    33421 tatccttcaa gcgcaaactt ctcaactgat ttgcactctt taggacaatt catccaagaa
    33481 ggacgtcgca atatttttga aacagttgtg aaggttgaaa aacctcgtaa atcaatcaca
    33541 attcctgaac aagcagaaga tttagacggt ctaggctact tgcaaggaaa agaagtggac
    33601 ttcgtaaata caaaagcatt tgaaggaaca ttgcttgctc atacagacgg tgacgtgcct
    33661 aacttgctag tcaaaattcc agaaatggat gcttatacat tgggttatac aatgtacttc
    33721 tttgaaattg ctgtaggtat ttcaggttac ttgaatggaa tcaacccatt cgaccaacca
    33781 ggtgtagaag cttacaagaa aaacatgttc gcacttctag gcaaaccagg ctttgaagat
    33841 cttgctaaag aattgaacga acgcctgtaa gataagggtt tttcgctaga tttaaataat
    33901 cagtgataga aacatgttcg ctttaaaatg aaaactggta cccatttagg tactcaaaca
    33961 tactgcatcg tttattcaga ataaggggct gtgacaagac gtttgtcaca gtcccttgtt
    34021 tgtatagaaa tcgattgatt gccttgctct ttagtttgtt ttaggacttt aagaacagtg
    34081 aggctgactt cgaaggttaa ttctgttttt ttgttttcta gaatcagtaa gcaaaaaagc
    34141 actcacttcg agtgcttttt tgcgatatat tcttagctta agataggcct aatttttctt
    34201 ttacagatga gggtacctta tcgctggtaa ttactttttt ctctatgatc cgcttttcgc
    34261 ttgctttcaa ctgcaagatc gttccttcgg ggtaagcaga accattggca gagtcaccag
    34321 aagtaatgac atcgaaatct aactgtcttt tttcaccgtt ttcagcataa gcatctacgt
    34381 tgtatttaaa acctcttcca attacttccc cattatctgc tttgatatca gtttctgcag
    34441 gcattggagc tttgactacc gcataataat ctttacctac ataagtagac tgatagtagt
    34501 cccaaccttt ccatcctata aaagcaatca atatgataac gatcaaccct aaaatttttt
    34561 tcatgaaaga tctccttttt tatttttatt atacccgtgg tgctagttaa ttttaatatc
    34621 ctaaagaata tctcaaagtt ccttcaaaac gtcgttgtgc tcgacttaat agaacactgt
    34681 aagccagtaa tatgctttca aatctactct ctagcccttt aacagaacgt gagttgaaat
    34741 tttgacaacc taatttttct aaggaagaaa aaacagtctc aactg
//
LOCUS       DACDKM010000017        34388 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20834_2016
            SAMN12640370-rid9891663.denovo.017, whole genome shotgun sequence.
ACCESSION   DACDKM010000017 DACDKM010000000
VERSION     DACDKM010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640370
            Sequence Read Archive: SRR10040891
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 34388)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34388)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742835.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742835.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:04:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,012
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 29 of 75
            Pseudo Genes (incomplete)         :: 33 of 75
            Pseudo Genes (internal stop)      :: 19 of 75
            Pseudo Genes (multiple problems)  :: 5 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34388
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640370-rid9891663.denovo.017"
                     /isolate="E20834_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            15..896
                     /locus_tag="IV334_000855"
     CDS             15..896
                     /locus_tag="IV334_000855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAP8942946.1"
                     /translation="MDIHKFEVFLDLAETMNFTKTADRQFTTQGNISKQILSLETELD
                     VKLFERAHRKIELTEAGSLLLPYVKNVVEQYHAMQEVLETYTKDNNLSLNILTIPTMI
                     NYKGFSKITEFLKRHPEFTVQLKEVESVELTYNDANEHSDTIHFARNFQAPPSSIEWL
                     PTETDDFVAVLPKNHPLASKKKLDLSELKKENFLLLGPTTNLYQPVLDLCREAGFEPK
                     ISYKGARIDLIINMIANDMGIAIVMEKTVKNLLKENTVILPISPTTESYLAFTRKVGE
                     HSLASDTFWEYLNQEEN"
     gene            complement(1133..2176)
                     /locus_tag="IV334_000856"
     CDS             complement(1133..2176)
                     /locus_tag="IV334_000856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAP8942947.1"
                     /translation="MKKKLFSQLWIYFASIVFISILVTLLCFLGFIFLLSRHSISPAE
                     SAGKPTLYPLFVFAGFSMVVGTGISIFVGRRILHPISALGTNMSLVATGDFSIRMDEQ
                     QKVAEVQQLYKDFNVMVQELNSIETLRNDFVSSVSHEFKTPLATIQGYVQLLQAPNLS
                     DEERQIFLYRIIESITQLSQLTENILKLNKLENQRIQLEKKEYRLDEQIREVIVFLQP
                     KWEKEQLELDIELAAVNYTGNEEFLYQVWLNIMDNAIKYNQINGQIHIKLFETATEIG
                     LEVTDSGVGMNEETRDRMFEKFYQGDTSRQISGNGLGLSLVKKILELHDGRIDYSSIE
                     GVGTTAMIRLSKQ"
     gene            complement(2179..2847)
                     /locus_tag="IV334_000857"
     CDS             complement(2179..2847)
                     /locus_tag="IV334_000857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP8942948.1"
                     /translation="MNRILVVEDDPQLQLLYRSVLERAGFSVITALNGEDALKKLEAT
                     RIELIITDIMMPKMDGYDLLDSLRSTRFDTPVLMITAKADFEDKKRGFKLGADDYMTK
                     PIDVNEMILRVEALLRRSKINYSSSLIIGQTKLELDAYTVLQDQHSLILPQKEFLLLY
                     KLLSYPNKIFTRQQLMDDIWGLDTNTEERTIDVHIKRLRTRFEKTEDFQIITVRGLGY
                     KAVI"
     gene            2989..5325
                     /locus_tag="IV334_000858"
     CDS             2989..5325
                     /locus_tag="IV334_000858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAP8942949.1"
                     /translation="MLQLKDIKKYYKVGETTTKALDGVSVAFRQKEFVAILGPSGSGK
                     TTMLNVIGGLDNYDSGDMVINGKSTKDFKDSDWDAYRNNSIGFIFQSYNLIGHLGIIE
                     NVELGMTLSGVSKDEKRKRAEDALHRVGLTDHMHKKPNQLSGGQMQRVAIARALANDP
                     DILLCDEPTGALDTETSIQIMELIQELSKEKLVIMVTHNPELAHQYADRIIEFSDGKI
                     LTDSHPHIERPKDDQFNLRRTKMSFWTALKLSFNNIRTKKGRTFLTSFASSIGIIGIA
                     IVLSLSSGFQKQIDNTQAETMAKFPITISKVTTNQTRDDAGLGASKADYPDSKTITAK
                     VSEEDKAQHTNKIDQTYVDYVTDIDPNLSNNIGFTRTTGINLLRDVNGKVQPVSFSNQ
                     NPDTESLSLSSTMSAMTGVGVSSFPTQLDTSKENFLKDNYSLLAGSYPASATDVVLIV
                     DGNNNTNINALKNLGFDVKEDEKLDFDDIVGTTFKLVNNNTYYTKLPTGNFIPNTDYD
                     AMYQNASDELKISGILRVKSSSTMNLLSPGIAYSDQLTTQIVNENKESEIVKAQRDSG
                     VNVLTTEKVDENAKQTLLSYLGGDSLPSSIMIYPNNFEDKEKILDYLDDYNKGKSDED
                     KIIYTDLAGTMTELTGGLMDAITYVLIAFAGISLVTSMIMISIITYTSVIERTKEIGV
                     LKALGARKKDITRVFDAETCILGISSGILGVFIAWLATFPINSILYNMTDLKNVAQLN
                     PVHAIILVIVSTILTMLGGHLPARMAAKKDAAIALRAE"
     gene            complement(5440..5751)
                     /locus_tag="IV334_000859"
     CDS             complement(5440..5751)
                     /locus_tag="IV334_000859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8942950.1"
                     /translation="MKKMKESFPKAVKVENIANILKVTFENGEVKYVKSHWTEEITDA
                     LQFGKKGRGKRKNLLALSRNMWIGTEVTIEADGTVFINGKDRYTPEELWYKGKKSIPE
                     L"
     gene            5932..7407
                     /locus_tag="IV334_000860"
     CDS             5932..7407
                     /locus_tag="IV334_000860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase"
                     /protein_id="HAP8942951.1"
                     /translation="MTDQPYDLRKVIEELKTLPGQYHETDIEIDPEAELSGVYRYIGA
                     GGTVKRPTQEGPAMTFNNIKGFPGTRVNIGTMASRKRVGEILHHDYRTLGRFLKDAVE
                     NPIKPVKVDKADAPAQEVVHLATDADFDIRNILPAPTNTPEDAGPYITMGVVLGSDPE
                     KTMSDVTIHRMVLEDKDTIGMYIMPGGRHIGHFQKQFEALNKPMPITINIGLDPAIAI
                     GTTFEPPTTPLGYNELWCAGALRNEPIQIVDGIAVDEVGIARAEFIIEAEIMPNETMQ
                     EDINTNSGKAMPEFPGYTGDANPAVQVVKVKAVTHRKDNPIMQTTIGPSEEHVSMAGI
                     PTEASILALVDKAIPGKVVNVYNPPAGGGKLMTIMQIRKENEADEGIQRQAAILALSA
                     FKELKTVILVDEDVDIFDMNDVVWTFNTRFQGDKDIIVIPGMRNHPLDPSELPEYNPG
                     SIRFRGMSAKTIFDGTVPFDMKEHFVRAKFQEVEDWEKYLK"
     gene            7454..8038
                     /locus_tag="IV334_000861"
     CDS             7454..8038
                     /locus_tag="IV334_000861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiX family flavin prenyltransferase"
                     /protein_id="HAP8942952.1"
                     /translation="MEQLAQRKKKIVIGVSGASGTIYAINLVKKLKEYPMIETHGIIS
                     PWAKQNLKLESDLSLTEFESLFDYHYSNKDLGAAVASGSFLTDGMIIVPASMKTVAGI
                     SVGLGDNLIARAADVTMKEQRKLVIVPRETPLNAIHLENLTKLARLGVQIIPPIPAFY
                     NQPQTIQDLVNHHTMKLLDQFGIVNEHSKRWQGD"
     gene            8040..8459
                     /locus_tag="IV334_000862"
     CDS             8040..8459
                     /locus_tag="IV334_000862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid decarboxylase"
                     /protein_id="HAP8942953.1"
                     /translation="MEKETIVEVTKLGFTIQAAVKVIGKDLLITLTGGDTPHIGTVTT
                     FSRDTEMQTVRFPSHDGRFHKDDVLAEKIAVIIKTAVPGSCTITSGIHVDHISNAQIN
                     ASFPMSEELGYKILDWLEKIDFHIKEPVYYKDGEKTL"
     gene            complement(8740..9024)
                     /locus_tag="IV334_000863"
     CDS             complement(8740..9024)
                     /locus_tag="IV334_000863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1905 domain-containing protein"
                     /protein_id="HAP8942954.1"
                     /translation="MREIYSFESKIYASEVDKGGAYIIFPYDIRKEFGAGRVKVQATF
                     DNMPYEGSIVNMGVKNPDGSVCYILGLRKDIRKNIGKEIGDIVAVTVKQK"
     gene            complement(9376..10086)
                     /locus_tag="IV334_000864"
     CDS             complement(9376..10086)
                     /locus_tag="IV334_000864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4811 domain-containing protein"
                     /protein_id="HAP8942955.1"
                     /translation="MIIGVLIVSLLAFALTNVFAKKTWQTFLSLIFGLIFVASLSLIV
                     ANLSNHFGMEKVTETKTEKIVSSADSQGADMLLYKALGNGKEKVYLYRTNEKQEKPKA
                     TGTDNETNKVEKTDGDAEKVTKTTYWEYKNDMYKFWFNIADNNHEYDSRVNTFKIPET
                     WVELSTDQAAKLAELVKKQQSTMESEAKAYVQDGMVKAMTENPKMSKAEQEQRTKALA
                     AEFQQQAFAKLVKEAKGE"
     gene            complement(10083..11555)
                     /locus_tag="IV334_000865"
     CDS             complement(10083..11555)
                     /locus_tag="IV334_000865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HAP8942956.1"
                     /translation="MSNNQPVDIYGKPYNRTLLVVVLLIGTFCTVLNQTLLTTAFPTL
                     MKAFDISASDVQWLTTGFLLVNGIMIPITAWLINKFSSRNLYLSAMTIFLIGTITCFT
                     APNFSTLLIGRLIQACGVGVSMPLLQNIMLSIFPPEKRGSAMGMSGIVIGLAPALGPT
                     LSGYIIDNYHWRDLFGMVLPIVVLVLVLAFFLMKSVIPLSNPSIDILSAILSTIGFGS
                     LLYGFSSVGDDGWGSLKVIGFLVVGVIFIALFSWRQLHLEHPFLELRVFKSSTFTIAA
                     VLAGVTNMAMVGAEMVVPLYIQNIRGESAFHSGLMLLPGALIMGAMMPVTGAIFDKYG
                     AKRLAITGMFILTAATAPFAFLTKTTPVLYIVILYAIRMFGISMVMMPVTTSGMNALP
                     NNLISHGTAVNNTFRQIASSIGTAILISVLTNVTKANIPSKSVLDNTPLAYKDQAINA
                     TLSGYHAAFIVAIIFGILGFIIAFLLKKKEPVLMKAGDNA"
     gene            11693..12166
                     /locus_tag="IV334_000866"
     CDS             11693..12166
                     /locus_tag="IV334_000866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HAP8942957.1"
                     /translation="MVGASLKRLLENDQLVVGISELSDIVGVSPRQLRYWEQKGFIRS
                     IEADANCPRKYRLPTVIKVELIKKYLDDGFTLSKAAEKAEIRQKKMHHVRKVFSKAIK
                     DIELIDERYTILQIGNFKEENAKMYIIHDNETDELTYKLLPVDEKADYEQLLKEL"
     gene            complement(12319..12870)
                     /locus_tag="IV334_000867"
     CDS             complement(12319..12870)
                     /locus_tag="IV334_000867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HAP8942958.1"
                     /translation="MARKNTLRYILLGLLSKKKMTGYELNQSFKNEIGEFWQAKHSQI
                     YPELAKMEEQGIIQHQVEITGEKLEKKVYELTEEGKEQLAEWIHTPTNELPVNRDEFV
                     LKLYFVKDVNDPALVEIIQQQRDLHEEKRLHLLERKKTIFPTEKEKHENYGHYLILAH
                     AINRETEYANWLTDVLEESNRKK"
     gene            12975..13505
                     /locus_tag="IV334_000868"
     CDS             12975..13505
                     /locus_tag="IV334_000868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenolic acid decarboxylase"
                     /protein_id="HAP8942959.1"
                     /translation="MKEFKELEDFIGTHFIYTYDNGWEYEWYAKNDHTVDYRIHGGMV
                     KGRWVKDQEADIVKLTDGVFKITWTEPTGTDVALDFMPNEKKLHGMIFFPKWVEEHPE
                     ITVTFQNEHIAEMEAAREKYETYPKLLVPEFATITYMGDAGIDNEDVISEEPYDGMPD
                     DIRSGKYFDENYKRVK"
     gene            complement(13988..14431)
                     /locus_tag="IV334_000869"
     CDS             complement(13988..14431)
                     /locus_tag="IV334_000869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP8942960.1"
                     /translation="MIRTTTKLSAKELESVLSIWLHANLDSHEFIPSTYWENQVESVR
                     KALPDAVIYLAEIDQEIIGFAGVIDQFIAGIFVKREYRQQRIGTQLMKKIKETNKTLT
                     LTVYQKNQSAIHFYLSQGFTIQTQQKDEAVNEEELVMKWNQNADF"
     gene            complement(14447..15607)
                     /locus_tag="IV334_000870"
     CDS             complement(14447..15607)
                     /locus_tag="IV334_000870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HAP8942961.1"
                     /translation="MKQSKQKQSRRLLFRLFLLICIVVGLLIALYPFYVDSLNSFIDQ
                     KRIEQVQKRTAAEIEVQRKKMEEKKQRLTDQGFNPGTDPFNEKNRNESTTSSQLEEWL
                     IGSINIPKIQLNISLYDRLNGRILENGAGVLQGTSFPLGGNSTHSVISAHSGLPNRRL
                     FTELDRLENGDTFILTVLGEKLAYQVKNIQVVLPDDTSVLTISEGEDLVTLLTCTPYM
                     INTHRLLVTGHRIPYNESVKKEEGKGNQERTMRQWFILAGTIIAVVILLVFIGRLIYQ
                     YRLSKKVLDFSFIISDSSGKPVNGSSFILKHKKKTLTRNGVPFSVQSDHHGKVKLDQL
                     PGGTYRIVSADDPKVAVSFGIRKVKQEKIYFFEGRKLVKELQKNGFYFKINE"
     gene            16114..17814
                     /locus_tag="IV334_000871"
     CDS             16114..17814
                     /locus_tag="IV334_000871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8942962.1"
                     /translation="MMNLSKVCKGVRQLKKYGPFLAIFFIALAFLMPHLFTHKLILGA
                     DAPFHYNRFYDAAQQIKYGDFHYFPSLYGFQQSGRIVNAVYGPFFAYLQGLIVLLAGS
                     WFHYQILSNFILYLISGFSMLILLRYVNVRNWVSVPVAVLFMSTYSIQYWSINQGFTS
                     WGTCFYPLCMIPIADFVLHKKFPVIKVAVSVGLMTQIHLLSSIMLALMYIPFYLYYFF
                     NQTEKGKALLQLIKSVLLYFVLTANIWAGLAIVYSGNKILPPFVNREMSQKAIDLDGS
                     YWLHYPKAFPILILSGFLLFIFFQQKNTLFQKIVLYTSLVFFFLSTSLIPWTTLIEKN
                     VPFISLIQFPFRFFVPFTLLLLLYLALSLNQWSEQKWFRLLSIFGILIFSAQTIQNLY
                     QHLEKWENETFVSRHTYLFDTPEEARKTFFSKDMSTSLFVFQKTTPDYLPIYKETKDN
                     KYDRYNEEILENETTFIKTHPGGLLTIKWKNTGDKTVHIPVIVYDRTVLQQNGKTLTD
                     YEVTDIGTPIVKQKKGINELTLHYQTPIYFYFILSLTLIGWFTLLCLFIYHRYKLLRA
                     "
     gene            complement(17916..18749)
                     /locus_tag="IV334_000872"
     CDS             complement(17916..18749)
                     /locus_tag="IV334_000872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HAP8942963.1"
                     /translation="MKFMTLNTHSWLEPEPEKKLQELADKILLEDYEVIALQEINQLL
                     ESEEVEPAKLMKFCQVKNQVPIRKDNFAYRLVQLLKEHGKEYFWSWEMSHIGYDKYEE
                     GNALLTKKTLESQVLTVSQSQKKEDYQTRKILIGKTKIDDQDIFVSSCHFSWWTDKKS
                     GFYFEWKNLENYFLETRVPLFFLGDFNNPVDSQGYYTVRESCLLLQDSYVVANEKGKA
                     ATVEKKIDGWEQNTEKLRIDFIFVPEGMQVKKYQRIFDGIDSPIISDHYGVEIELDVN
                     E"
     gene            complement(19053..21218)
                     /locus_tag="IV334_000873"
     CDS             complement(19053..21218)
                     /locus_tag="IV334_000873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP8942964.1"
                     /translation="MKKIFSFEFWQKFGKALMVVVAVMPAAGLMISIGKSIPLINPDW
                     TPLVTTGGVIENIGWAIIGNLHILFALAIGGSWAKERAGGAFAAGISFILINRITGAI
                     FGVTSDMLANEDAFTHTLFGTKIMVKGFFTSVLEAPALNMGVFVGIIAGFVGAMAYNK
                     YYNYRKLPDALSFFNGKRFVPFVVILWSTIVALILAVVWPNVQAGINNFGLWIAESQE
                     SAPILAPFLFGTLERLLLPFGLHHMLTIPINYTQLGGSYEILSGAQAGTQVFGQDPLW
                     LAWATDLVNLKGAGDMSQYEFVLHNWTPARFKVGQMIGSSGILMGMAFAMYRNVDPDK
                     KARYKSMYFSAALAVFLTGVTEPLEFMFMFAAVPLYVIYSVIQGAAFAMADILPLRVH
                     SFGNIELLTRTPLAIKAGLGGDLINFVLCVIAFGVVTYFLANFLIKKFNFATPGRNGN
                     YDNDSEETVVSNSGTGTADQQVVQIIHLLGGKENIKDVDACMTRLRVSVNDREKVGTE
                     EAWKRAGAMGLIVKDNGVQAVYGPKADVLKSDIEDLLQSGAEIPEPEMVSTGNGQAGS
                     KQYLGIEQELVSAASGEVIPLSEVKDPVFSQKMMGDGFAVIPTSREVVAPIAGKVTSI
                     FPSKHAIGLETKDGIEVLIHMGIDTVQMNQPAFEIAVKEGQEVGTGTKLAEMDLDVIQ
                     NEEKDPTIMIVFTNDKVEEVVIKQLGTVTAGKVIGSIKM"
     gene            21478..23772
                     /locus_tag="IV334_000874"
     CDS             21478..23772
                     /locus_tag="IV334_000874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 65 protein"
                     /protein_id="HAP8942965.1"
                     /translation="MKQIKRLFAIDPWKISTTQLDKKNLRLQESLTSIGNGYMGMRGN
                     FEETYSGDHHQGTYLAGVWYPDKTRVGWWKNGYPEYFGKVINALNFIAIDISINDQLI
                     DLASLEPEDFYWELDMKNGVLSRTFTITTSTNKVRISFERFLSIVKKESAYIYLTIEM
                     LEGSGTVKLVSKLDGNVQNEDSNYDEHFWNEIDRGTIEEIGYLTTKTIPNNFGIEQFT
                     VTAAMRHFVNGKSVIPDYHEDTLALHGCLSAELSQGETWSVEKEIIVLTSRDIPEAQQ
                     AEKAVSELTNQTNNYIQAKEEQTAAWAERWKLADVVIEGDEEAQQGIRFNLFQLFSTY
                     YGEDERLNIGPKGFTGEKYGGATYWDTEAYAVPLYLALAKPEVTKNLLKYRHNQLPQA
                     IHNAQQQGLKGALYPMVTFTGVECHNEWEITFEEIHRNGAIAYAIYNYTNYTGDTEYL
                     KNEGLEVLAEIARFWADRVHFSKRANKYMIHGVTGPNEYENNINNNWYTNTIAAWVLR
                     YTRENYLTYQKEAAITISEEELAKWKDIAENMYFPVDEELGIFVQHDTFLDKDLMPVS
                     DIPTSDLPLNQHWSWDKILRSCFIKQADVLQGIYFFNDTFTKEEKQKNFDFYEPMTVH
                     ESSLSPSIHSILAAELGMESKAVEMYQRTARLDLDNYNNDTEDGLHITSMTGSWLSIV
                     QGFAQMKTAHDQLRFAPFLPKTWKAYSFHINYRGRLLFISVTDEQVTFELLEGFPLEI
                     YVYEQSYSLADRLEITLRKEEANV"
     gene            23765..24430
                     /gene="pgmB"
                     /locus_tag="IV334_000875"
     CDS             23765..24430
                     /gene="pgmB"
                     /locus_tag="IV334_000875"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HAP8942966.1"
                     /translation="MFKGVLFDLDGVITDTAEFHYRAWRKLGQEIGITIDHTFNEQLK
                     GVSREDSLSLLLAYGGKEHSFSKEEFAELAKRKNDYYLEMIQTIEPKDVFPGVVPLLD
                     SLKEEKIKIALASASKNGPFLLEKMGLTPYFDAIADPAEATNGKPAPDIFLLAAKAVG
                     LKAEECIGIEDAQAGIQAILSSGAQPIGVGRAEDLGEEIPLVPDTTFLTIDYLKEKCH
                     DHG"
     gene            24423..25430
                     /locus_tag="IV334_000876"
     CDS             24423..25430
                     /locus_tag="IV334_000876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose mutarotase"
                     /protein_id="HAP8942967.1"
                     /translation="MDNPEISVVPVPETEIKKITLKNDYLEVVLLNYGARLHQIFAPD
                     KEGKSENVLLSYDRFEDVLTDKSFFGATVGPVAGRIRDASWGEHRLEKNCGSHHIHGG
                     TKGWSFQFWEVEVFKTPSSIGVVFYLKDEFSTYPGPITATITYRLTKNELEMTTSASS
                     LVETICNPTNHAYFNLSGNGTRDIYEHQLTVFLDGVLELDQEKLPTGNWTKKEKLPID
                     FRKSPTLQEILACYPDGLDDVFLLHHPRLSKTSLQLFEKHSGRQMTIATSNKSMVLFS
                     TTGFEADFSVNGKQMHSNYGLAIEPQEIPDIVHFPKFGSINLHPGQERISQTIYRFSA
                     Q"
     gene            25452..26471
                     /locus_tag="IV334_000877"
     CDS             25452..26471
                     /locus_tag="IV334_000877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAP8942968.1"
                     /translation="MAITVKDVAKKAGVATSTVSRVINDHPSISETTKKKVRKIMDEL
                     GYVPNMTARNLGKRISSAIGVILPPLDSKERIGNPFYLEIMEAINEEARNYDMTTAIA
                     TAKNFELLLENVQRMHLQKQVDGFILVYSDSQDPVIDYLYQNHIPFTLIGQPYHHENE
                     IVYVDNDNQLLGKQATEFLIQNGHKNILFVTNTTHESLYFERYFGYQKAMMMAGLPAL
                     SSVVLEHPEDYLSFEKTLKENEATALVVIDDIFALRTMQLAQMYGYVIPETLSVISFN
                     NSIFSTLTHPYLTSIDIDISELGRVAMQKLRELIHEELSSGVRLVIPHRLIKRETVVP
                     LENPY"
     gene            complement(26752..28119)
                     /gene="nhaC"
                     /locus_tag="IV334_000878"
     CDS             complement(26752..28119)
                     /gene="nhaC"
                     /locus_tag="IV334_000878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HAP8942969.1"
                     /translation="MNKNISLKESIAVLIVLLALLGVLIIGFQLSPHIPILAAFMCLL
                     FYGRFKKFTWDEIHDGIIKGITPGIIPILIFLLIGVLVASWILSGTIPTIMVYGFKLV
                     SVRFFLPTAFLVCAIVGVTVGSSFTTISTIGIAFLGIGHLLGFNDAMTTGAVVSGAFL
                     GNNCSPLSDTTNLAAGIGGVNLFEHIAGMRYTDFPAFVLSLIFYTILGHNSHSADLAS
                     INEMIQNMKASFWISPWTLIPLVILFGGAIKKIPAIPTLLAGSTAALVLSFIHEKSLT
                     VGKAADILMNGYVANTPDPKLDELLSRGGIMSMLGSASLILLALALGGLLIKYLIVDT
                     IVQELKEKMDRPSRLVGFTALSCIGINLIVGEQYLSIILPGETFKRSFDDVGLSKTYL
                     TRTLADSGATVNSLVPWGVSGTFIMGTMKVSALHYLPFVFFSILAPVFTILGGFLLNR
                     RRVKN"
     gene            complement(28229..29650)
                     /locus_tag="IV334_000879"
     CDS             complement(28229..29650)
                     /locus_tag="IV334_000879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAP8942970.1"
                     /translation="MSKSEVSKMGLGTFIGLTMALCATVRSIPTLAAVGWTLIFYSIF
                     AVLFFAGPISMVSGELSTMLPQEGGPQLWVKTALGSKWGFVVAWLLWVQMFPGMVMVA
                     STLGPLLGNTFGNVELGNNHWFVLGCILVIYWIITILNLKFDMAKVGGNIGVWLGVYI
                     PVVIMFVLGVLAAFKVGLVSNGYLGDFSWSKAFPDLEHIDSLKYLAGITFIFVGIEMS
                     SVYMPRLKDATKNYTKGVFIALIGLVLLNVINAMLVANVVPDGKMELANITQPILIDC
                     QILGLPEVIGNIFSFMVFIGVLLQLSAWVTGPSKTIIQVAREGFLPPKFGFHKENKYG
                     VSRNVVLTQSIVISLFALLYGVMDDVSAVFLTLTNATTVIYCIVYILIAVSLLKMRKK
                     HPEFERPYRIGKNGNGLAWVVSCMLIFSIIVVVFATLGTATLSDALLVAAITVVMFVI
                     PLIINHFKKDSWGIEVEKSLEEK"
     gene            complement(29893..31770)
                     /gene="tdc"
                     /locus_tag="IV334_000880"
     CDS             complement(29893..31770)
                     /gene="tdc"
                     /locus_tag="IV334_000880"
                     /EC_number="4.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine decarboxylase"
                     /protein_id="HAP8942971.1"
                     /translation="MSESLSKDLNLNALFIGDKAENGQIYKALLNELVDEHLGWRQNY
                     MPQDMPIITPEEKSSASFEHTVNKTKDVLSEISARMRTHSVPWHNAGRYWGHMNSETL
                     MPSLLAYNFAMLWNGNNVAYESSPATSQMEEEVGMEFAKLMSYKDGWGHIVADGSLAN
                     LEGLWYARNIKSLPLAMKEVTPELVAGKSDWELMNLSTEEIMNLLDSVPEKIDEIKAH
                     SARSGKHLEKLGKWLVPQTKHYSWLKAADIIGIGLDQVIPVPVDHNYRMDINELEKIV
                     RGLAAEKTPILGVVGVVGSTEEGAIDGIDKIVALRRVLEKDGIYFYLHVDAAYGGYGR
                     AIFLDEDNNFIPFEDLKDVHYKYNVFTENKDYILEEVHSAYKAIEEAESVTIDPHKMG
                     YVPYSAGGIVIKDIRMRDVISYFATYVFEKGADIPALLGAYILEGSKAGATAASVWAA
                     HHVLPLNVTGYGKLMGASIEGAHRFYNFLNDLSFKVGDKEIEVHPLTYPDFNMVDYVF
                     KEKGNDDLVAMNKLNHDVYDYSSYVKGSIYGNEFLTSHTDFAIPDYGNSPLQFVNQLG
                     FSDEEWNRAGKVTVLRASVMTPYMNKEEHFEEYAEKIKAALQEKLEKIYADQLLASEA
                     K"
     gene            complement(32061..33317)
                     /locus_tag="IV334_000881"
     CDS             complement(32061..33317)
                     /locus_tag="IV334_000881"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HAP8942972.1"
                     /translation="MNIIDELTWRDAINQQTNEERLRELVEEKSISLYCGVDPTGDSM
                     HIGHLIPFMMMKRFQLAGHHPYILIGGGTGTIGDPSGRKTERVLQTMEQVQHNVDALS
                     NQMRKLFGKDANITFVNNYDWLSKISLLEFLRDYGKNFNINTMLAKDIVASRLEVGIS
                     FTEFTYQILQSIDFLHLHKTYDVQLQIGGADQWGNITAGLDLIRKLEGPEVEAFGLTI
                     PLMLKADGTKFGKTAGGAVWLDPKKTSPFEFYQFWLNQDDRDVVKYLKFFTFLSQEEI
                     EDLAKKVETEPEKREAQRRLAEEVTRFVHSEEDLKEAQKITQALFSGNIKELNAEEIA
                     QGFGKMPNVEISSTPENIVELLVSTKIEPSKRQAREDVSNGAISINGDRVTDLNFVIN
                     PSDEFDGKFVVIRKGKKNYFLAKVID"
     gene            33897..>34388
                     /locus_tag="IV334_000882"
     CDS             33897..>34388
                     /locus_tag="IV334_000882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8942973.1"
                     /translation="MEKKQKIILGATSALTIVILILSLFFVIRSSTKKEPIIPEKKIT
                     EKAKEPTDDKQQLVTQAKNLAQSYDYDQAIALLEKDSKNGSTETQQLLQTLKKEKEQL
                     IDWSDPTQISHVFFHSLIVDPQKAFHSQQAQGYKDYMVTVEEFNRSIEQLYQNDFVLV
                     NLND"
BASE COUNT        10552 a         7053 c         6214 g        10569 t
ORIGIN      
        1 gtaggaggaa tatgatggat atccataaat tcgaagtttt tcttgattta gccgaaacaa
       61 tgaatttcac caaaactgct gatcgacaat tcaccaccca aggaaatatt tctaaacaga
      121 ttctttcgtt agaaacagaa ttagacgtca aattattcga acgcgcccat agaaaaatcg
      181 aattgaccga agctggaagc ttattgctcc catatgtaaa aaatgtcgta gaacaatacc
      241 atgctatgca agaagtgctt gaaacctata cgaaagacaa caatctttca ttaaatatct
      301 taacgatacc gacaatgatc aattataaag gtttttccaa aatcaccgag tttttgaaac
      361 gccatcctga atttacagtc cagttgaaag aagtggaaag tgtcgaatta acttacaatg
      421 acgccaatga acatagtgac acgatccact ttgctagaaa tttccaggcc ccaccaagtt
      481 cgattgagtg gttgcctact gaaacagacg attttgtagc agttctacct aaaaatcatc
      541 ctttagctag taaaaagaaa ttggatctaa gcgaactgaa aaaggagaac tttttgctcc
      601 ttggccccac tacgaacttg tatcaacctg tactagatct gtgtcgcgaa gctggatttg
      661 aaccaaaaat ctcttataaa ggagcaagaa tcgacttgat catcaatatg attgcaaatg
      721 acatggggat tgctattgtt atggaaaaaa cagtcaagaa tctattaaaa gaaaatactg
      781 tgattctccc tatttctcca acaacagaga gttatcttgc atttactcgt aaagtcggtg
      841 agcattcttt ggcttcagat acattttggg agtatttaaa tcaagaagaa aactaagccc
      901 tacattttct gtccatctat ccacccaaaa aaagagagcg acgcatgact ttcgctctct
      961 ttcttgcatc atgtccttgt ttcaatgaca cctctaacat ttctcgtagt tcaataagaa
     1021 agcagcgatt tttatgccaa aagaaccgta agaacatttc cctaatcaca aaaaaacgac
     1081 taaaaacccc taaaaacctt caatagaatt tttaggggtt ttattctttt tcctattgtt
     1141 tacttaaccg tatcattgct gtcgttccta ctccttcgat actgctataa tcaatacgtc
     1201 catcatgcag ttctagaatt tttttaacca gtgaaagtcc tagcccattc ccagaaattt
     1261 gacgacttgt atctccttga taaaattttt caaacattcg atctctggtc tcttcattca
     1321 ttcctacacc tgaatcggtc acttccagtc caatctctgt ggctgtctca aatagtttga
     1381 tatgaatctg tccattgatt tgattgtact ttatggcgtt atccatgata ttcagccaca
     1441 cttggtacaa aaactcttca ttgcctgtgt aatttactgc agcaagttcg atatccaatt
     1501 ctagctgttc tttctcccat ttaggctgca aaaaaacgat tacttctctg atttgttcat
     1561 ctaatcgata ctctttcttt tccagttgga tacgctggtt ttccagtttg ttcaatttta
     1621 agatgttctc tgttaattgt gaaagctgcg tgatactttc gataatccga tataaaaata
     1681 tttggcgttc ttcgtcagag agatttgggg cttgtaacaa ttgaacatat ccttggattg
     1741 ttgctagtgg tgttttgaat tcatgggata cactggacac aaagtcattc ctcagtgtct
     1801 cgatactatt gagttcttgg accataacgt tgaaatcttt atacagttgc tgtacttctg
     1861 ctactttctg ctgttcatcc attcgaatcg aaaaatctcc cgttgctacc agactcatat
     1921 ttgtccctag cgcactgatt ggatgcagga tacgccgccc aacaaaaatc gatattcctg
     1981 tacccacgac catactgaac cccgcaaaga cgaataacgg gtataacgtt ggttttccag
     2041 ccgattcagc aggcgagata gaatgtcgag acaataagaa aataaaaccg agaaaacaca
     2101 gcagtgtcac taagatcgaa atgaagacaa tactagcaaa atagatccat aattgtgaaa
     2161 atagcttttt tttcatcttt aaataaccgc cttatagcct agtccccgta ctgtgatgat
     2221 ttgaaaatct tccgtctttt caaaacgggt ccttaaacgt ttgatatgta catcgatcgt
     2281 tcgttcttct gtattcgtat ctaatcccca aatatcatcc atcaactgtt ggcgagtgaa
     2341 gatcttgttg ggataagaca gtaatttata cagaagcaga aattcttttt gaggcagaat
     2401 caacgaatgc tgatcttgta acactgtata agcatctagc tctaattttg tttgtccgat
     2461 tatcaagcta ctgctgtaat tgatctttga acgtcgaagc aatgcttcca cacgtaggat
     2521 catctcattg acatcgatgg gttttgtcat ataatcatct gctcctaatt tgaacccgcg
     2581 tttcttatcc tcaaaatcgg cctttgccgt aatcattaga acaggtgtgt caaatctggt
     2641 gctacgtaat gagtctagca agtcatagcc atccattttc ggcatcatga tatctgtaat
     2701 gattaattct atacgtgtcg cttctagttt tttcagcgca tcctctccat tcaatgcggt
     2761 gatgactgaa aagcccgctc gttccaacac agaacggtaa agcaactgta attgcggatc
     2821 atcttctact actaaaatac gattcattga tctccctctg ttttttgaaa aatttctaga
     2881 ttttttcttg tagttcatct tcagttaaca tttatttcgt atactacgat cattgttctg
     2941 atatacttat tagtataccg aatatttcaa tacgggagaa gggtgattat gcttcaatta
     3001 aaagatataa aaaaatatta taaagtcggc gaaaccacca ccaaagcatt ggatggtgtg
     3061 tctgtcgctt tcagacaaaa agaattcgta gcgattttag gtcctagtgg ttctggtaaa
     3121 acgactatgc tgaatgtgat tggtggacta gataattatg attccggtga tatggtaatc
     3181 aacggaaagt ctactaaaga ctttaaggac agtgactggg atgcctaccg aaacaactct
     3241 atcggtttca tttttcaaag ttacaacttg attggtcatc taggcattat tgaaaacgtt
     3301 gaacttggga tgacattaag tggtgtttct aaagacgaaa aacgaaaacg ggcagaagat
     3361 gcattgcacc gagtcggctt gacagatcat atgcataaaa agccaaatca gttatctggt
     3421 ggacaaatgc agcgagtggc catcgctcga gcactagcca atgacccaga tatccttttg
     3481 tgcgatgaac caaccggagc gttagataca gaaacaagta tccagatcat ggaattgatc
     3541 caagagttat cgaaagaaaa attagtgatc atggtcacac ataatcctga actggcccat
     3601 caatacgcag atcggatcat tgaattttct gatggtaaga tcttaacaga ttcccatcct
     3661 cacatcgaac gaccaaaaga tgatcaattt aacttacgca gaacaaagat gagtttttgg
     3721 acagcgttaa agctttcctt caacaatatc cgaacaaaaa aaggacgtac tttcttgacc
     3781 tcctttgctt ccagtattgg gatcatcgga atcgcgatcg ttctgtccct ttcttctgga
     3841 ttccaaaaac aaatcgataa cacgcaagct gaaacgatgg cgaaatttcc gattactatt
     3901 tctaaggtaa cgactaacca gactcgtgac gatgccggat tgggtgcaag caaagcggat
     3961 tatccagaca gcaaaacgat tactgcaaaa gtcagcgaag aagataaagc acagcacacg
     4021 aataagattg atcaaacata cgtcgattac gtaacagaca tcgatcctaa cttaagtaat
     4081 aatatcggat ttacccgtac tacaggaatc aatcttttac gtgatgtaaa tggcaaagta
     4141 cagcctgtca gcttctcgaa tcaaaatcct gatacggaat ctctttcttt atctagtacg
     4201 atgtcagcaa tgacaggtgt gggagtgtca agtttcccta cacagcttga cactagcaaa
     4261 gaaaactttt taaaagacaa ttattcttta cttgccggaa gttatccagc ctcagcaaca
     4321 gatgtcgtgc tgatcgtaga cggcaataat aatactaata tcaacgcatt gaagaatctt
     4381 ggatttgatg tgaaagaaga cgaaaaactt gattttgatg atatcgttgg tacaacattc
     4441 aaattagtaa acaacaatac ttactataca aaacttccaa ctgggaattt tatccctaat
     4501 acagattatg atgcgatgta tcagaatgct tctgatgaat tgaagatctc ggggatccta
     4561 cgcgtgaaat cttcttctac aatgaatctc ttatctccag gaatcgctta tagcgatcaa
     4621 ttaacgactc aaatcgtcaa cgaaaacaaa gaatcagaaa tcgtcaaagc tcaaagagac
     4681 agtggtgtaa atgtcttaac tactgaaaaa gttgacgaaa atgccaaaca aacattgctt
     4741 agttatttag gtggagacag cttgccttca agtatcatga tttatccgaa caattttgaa
     4801 gacaaagaaa agatcttgga ttatctagat gattacaata aaggcaaatc agatgaagat
     4861 aaaatcattt atactgattt agctggaacg atgactgaac tgactggcgg attgatggat
     4921 gctatcactt atgtccttat tgcttttgca gggatctctt tagtaacaag tatgatcatg
     4981 atcagtatca ttacttatac atctgtgatt gaacggacaa aagaaatcgg tgtactaaaa
     5041 gctttaggtg cacgtaaaaa agacatcact cgcgtatttg acgcagaaac gtgtatttta
     5101 ggtatttctt ctgggatatt aggtgtgttt attgcatggc tagctacgtt ccctatcaat
     5161 agcatcttat acaacatgac ggatctaaaa aatgttgctc aactaaatcc tgtccatgcg
     5221 attattctag tgatcgtctc tactatattg acgatgcttg gcggtcatct tcctgcacga
     5281 atggcagcga aaaaagacgc cgctatcgct ttgcgtgcag aataaggttg tgacaaccac
     5341 atttagattt gagtcataag agcgctgaaa tagtagaaaa cagcaagaaa cgaggaagta
     5401 atatgttcct tgtttcgcgc tgtttttttt aattattttt tataattctg gtatactttt
     5461 ctttcctttg taccacaact cttctggcgt atacctgtct tttccattta tgaatactgt
     5521 tccatctgct tcaatcgtta cttcagtccc aatccacata ttacgagaaa gtgcgagaag
     5581 attttttctt tttcctcttc ctttttttcc aaattgcaag gcatcggtta tctcttctgt
     5641 ccaatgagat ttcacgtatt ttacttctcc gttttcaaat gtaactttca aaatattcgc
     5701 tatattttcc acttttactg ctttaggaaa tgactccttc atttttttca ctttccttcc
     5761 tccaacacta agtatgcgct ttcttttagt gataagtata tcatagttta ttctattatc
     5821 aaaaaaacgc atccattcca ttgtagaata ctttaccgaa caagttgaat tgttttcctc
     5881 tttttcacct tttactatag ataggtaaag acaactaagg aggaatctct catgacagat
     5941 caaccgtatg atttaagaaa agtcatcgaa gaactaaaaa cacttccagg tcaatatcac
     6001 gaaacagata ttgaaatcga tcctgaagca gaattatctg gtgtttaccg ttatatcggt
     6061 gctggcggta cagtaaaacg tccgactcaa gaaggaccag ctatgacttt caacaatatc
     6121 aaaggtttcc ccggcacacg tgtgaacatc ggaacaatgg ctagtcgtaa acgtgtaggt
     6181 gaaattttgc accatgatta tcgaacactt ggtcgtttct tgaaagacgc tgtagaaaat
     6241 ccaatcaaac ctgtgaaagt agacaaagca gacgctccag cacaagaagt cgttcatcta
     6301 gctaccgatg cagacttcga tattcgcaac attttgcctg cacctacaaa tactcctgaa
     6361 gatgctggtc catacatcac aatgggtgta gtcttaggat cagatccaga aaaaacaatg
     6421 agcgatgtaa cgatccatcg tatggttcta gaagataaag atacgatcgg tatgtacatc
     6481 atgcctggcg gacgccacat tggtcacttc caaaaacaat tcgaagcctt gaataaacca
     6541 atgcctatca ctatcaatat tggtttagat ccagcaattg ccattggcac gactttcgaa
     6601 ccacctacca ctccacttgg ctacaatgaa ctatggtgtg caggagcttt gcgtaacgaa
     6661 cctattcaaa tcgttgatgg tatagcagtc gatgaagtgg gtattgctcg tgctgaattc
     6721 atcattgaag cagaaatcat gccaaatgaa acaatgcaag aagatatcaa cacgaactca
     6781 ggaaaagcaa tgccagaatt tcctggctat actggggatg ctaatccagc tgtacaagta
     6841 gtgaaagtca aagcagttac tcaccgaaaa gacaatccaa tcatgcaaac aacgattggt
     6901 cccagtgaag aacacgtatc tatggccggt attcctacag aagcaagtat tcttgccttg
     6961 gtcgataaag ctattccagg aaaagtagta aatgtctaca atcctcctgc aggtggtggt
     7021 aaattgatga ccatcatgca aatccgtaaa gaaaacgaag cggatgaagg tattcagcgt
     7081 caagcagcta ttcttgcttt atcagccttc aaagaattga agacagtcat tttagtcgat
     7141 gaagatgttg atattttcga catgaacgat gtagtttgga ctttcaacac tcgtttccaa
     7201 ggagataaag atatcatcgt gatcccagga atgaggaacc atccattgga cccatcagaa
     7261 ttaccagaat ataatcctgg ctctattcgt ttccgtggga tgagtgcaaa aactatcttt
     7321 gacggtacag ttcctttcga tatgaaagaa cattttgtac gtgcaaaatt ccaagaagta
     7381 gaagattggg aaaaatattt gaaataatga atgatctgca actgttttga catcataaaa
     7441 aggagtgcgg aaaatggaac aattggcaca acggaagaaa aaaatcgtga ttggtgtaag
     7501 tggcgcttca ggcaccattt atgccatcaa cctagtgaag aaactaaaag aatatccgat
     7561 gatagaaaca cacggaatca tcagtccatg ggcaaaacag aacttaaagc tggaatccga
     7621 cttatcacta actgaattcg aaagcctgtt tgattaccat tattcaaaca aagatttagg
     7681 tgctgctgtc gccagcggat catttttgac cgatggaatg atcatcgtac cggcaagtat
     7741 gaaaacagtc gcaggtatct ctgttggttt aggcgacaac ctgattgcac gtgctgctga
     7801 tgtaacaatg aaggagcaac gaaagttagt cattgttcct cgtgaaacgc cattaaatgc
     7861 cattcacttg gaaaacttga ctaaattagc acgactaggt gtgcaaatca taccaccaat
     7921 tcctgctttt tataatcagc ctcaaacgat ccaagatctt gttaatcacc atacaatgaa
     7981 acttttggat caatttggaa ttgtaaatga gcattcaaaa cgttggcagg gtgattaata
     8041 tggaaaaaga aacgatagtt gaagtaacca aactaggttt cacgatccaa gctgctgtta
     8101 aagtaatcgg taaagactta ttgatcactt taacaggcgg agatacacca catatcggaa
     8161 cagtcactac attttctcgt gatacagaga tgcaaactgt acgtttccct agccacgatg
     8221 gtcggttcca taaagacgat gtcttagccg aaaaaatagc tgtgatcatc aaaactgccg
     8281 ttccgggaag ttgtacgatc acttccggta tccatgttga ccatatctcc aatgcacaaa
     8341 tcaacgcctc ttttccgatg tcagaagagt taggttacaa gattttagat tggttggaaa
     8401 aaatagattt ccatatcaaa gaaccagttt attataaaga tggtgaaaaa acattatgaa
     8461 agaagaggtt gtgaaatcag cgttatgacc tgagtatgag agaaaaaaat ggaaaaatag
     8521 cttctcatat tttgccgttt ttgagctaat accgagggac agctgattga acgccgttta
     8581 tcaagaggtt gtgataaagc cgcttcgttt cgagtagtaa gaaaaatttt gtaaacatgg
     8641 gtttccacat ttttgcataa atttgactta ctaccgagaa actggctttt gaacaccgtt
     8701 tatcaagagg ttgtgacacg tcttttgtca caacctcttt tatttttgct tcactgtaac
     8761 tgctacaata tcaccgattt cttttccgat gtttttacga atatcttttc gtagtccaag
     8821 gatgtagcag actgaaccat ctggattttt cacgcccata ttcacgatac tgccttcata
     8881 cggcatattg tcaaaagttg cttggacttt aactcttcca gcaccgaatt cttttcttat
     8941 atcatacgga aaaataatat atgccccgcc cttgtcgact tcggaagcat agatttttga
     9001 ttcaaaagaa taaatttccc tcattttatt tctcctattt tttacatcta gtatagcata
     9061 gcaaaaaaaa gaaggaactg tgacaaaagt cttgtcgcag ttcctttttt cgaataaacg
     9121 gtgttctcct gccaactcct cggtcacaag tcacaatagt caacaaacat gagaagctgt
     9181 tttttgaaat tgctccttgt gactcagagt taaacggcag gctcacaacc tcttccttaa
     9241 acggtgttca aaagcttgca tctacggcac taagcccaat cagatgaaaa atatggagtg
     9301 ctatttttat ctgattgttc ttactgcttg atgcagagca gctttttcac aacctcctac
     9361 atggtgttca aaatattatt cgccttttgc ttctttcact aattttgcga atgcttgttg
     9421 ttggaattca gcagctaagg ctttcgtacg ttgttcttgc tctgctttgc tcatttttgg
     9481 attttcagtc attgctttta ccataccgtc ttgaacataa gcttttgctt cactttccat
     9541 cgtactttgc tgtttcttca ctaattcagc taacttggct gcttggtccg tacttaattc
     9601 tacccatgtc tctgggattt tgaatgtatt cactcggctg tcatattcgt gattgttatc
     9661 agcaatattg aaccagaatt tatacatgtc gtttttgtat tcccaataag tagttttcgt
     9721 cactttttca gcatcgccgt ctgtcttttc tactttattt gtttcgttat ccgtaccagt
     9781 tgcttttggt ttttcttgtt tttcatttgt tcggtaaaga tacacttttt cttttccatt
     9841 ccctaaagct ttatacaaaa gcatatctgc tccttgggaa tcagcactgg aaacaatttt
     9901 ttctgttttt gtttccgtta ctttttccat tccaaagtga ttggacaaat tagcgacgat
     9961 caaacttagt gaagcgacga agattagtcc gaaaatcaaa gataaaaagg tttgccatgt
    10021 ttttttagcg aacacgttcg ttaaagcaaa agcaagcaag ctgacaatca aaactccaat
    10081 aatcatgcat tatcccccgc tttcattaga acaggttctt ttttcttcaa taaaaaggca
    10141 ataataaagc caagaatccc aaagatgatc gctacgataa acgcggcatg gtaacctgac
    10201 agtgtggcat tgattgcctg atctttataa gctaatggcg tattatccaa tacagatttg
    10261 cttgggatat ttgcttttgt cacattcgtc aacacactga tcaaaatagc tgtaccaatc
    10321 gagcttgcga tttgacggaa tgtattattt accgctgtcc catgactgat caagttattt
    10381 ggcaatgcat tcataccaga agtcgttacc ggcatcatga ccatggagat cccaaacata
    10441 cggatcgcat aaaggatcac gatatataaa actggagtcg tttttgtcaa gaaagcaaat
    10501 ggtgcagttg ccgctgtcaa aatgaacatc cctgtgatgg ctaaacgttt cgcgccgtat
    10561 ttatcgaaaa tcgcacccgt cacaggcatc atagccccca tgatcaatgc acccggcaac
    10621 aacatcaagc cagaatggaa tgctgattct ccacgaatat tttgaatata caatggtacg
    10681 accatttctg cccctaccat cgccatattt gttacaccag ctaaaacagc tgcaatcgtg
    10741 aatgtagatg atttgaatac acgaagttcc aagaatggat gttccaaatg taattgacgc
    10801 catgagaaca aggcgatgaa aatcacacca acaactaaga aaccaattac tttcaagctt
    10861 ccccagccgt catctcctac actagaaaat ccgtaaagta aactaccgaa tccaatcgtt
    10921 gataaaatag cggataaaat atcaatgcta ggatttgaca atggaataac acttttcatt
    10981 aggaagaatg ctaaaaccaa gacaagaaca acgattggca gtaccatacc aaacaagtca
    11041 cgccaatggt agttatcaat gatataacca gataatgtag ggcctaaagc aggagcaagt
    11101 ccaataacga tccctgacat ccccattgct gatcctcttt tttctggcgg gaaaatagat
    11161 agcatgatat tttgcaataa tggcatcgat actcccacgc cgcaagcttg gatcaagcga
    11221 ccgattagta atgtactaaa attaggtgct gtaaagcaag taatcgttcc gatcaaaaag
    11281 atcgtcatgg cagaaagata taaatttctt gaactgaatt tattgatcag ccatgctgtt
    11341 attgggatca tgatcccgtt gactagcaag aaaccagtcg ttaaccattg cacatcagac
    11401 gctgaaatat cgaaagcttt catcaatgta ggaaaagctg tcgtcaataa ggtctgattc
    11461 aaaacggtac aaaatgtacc aattaataag acaacaacta aaagtgtccg attatatggt
    11521 tttccgtaaa tatctacggg ttggttgtta ctcatatttc tccttctttc tgaatttttt
    11581 ctgaaactac tagaatactt tagtcctaaa ttcgctattt gtcaacctac ttgacacata
    11641 agttaatacg gatataatta agggaagcaa gaggacaaaa ggagtgacaa aaatggttgg
    11701 ggcttccctt aaacgtttgt tggaaaatga tcaattggtt gttggcatca gtgaattaag
    11761 tgatatcgta ggtgtgtctc cacgtcaatt aaggtattgg gaacaaaagg gattcattcg
    11821 ttcgatcgaa gctgatgcga attgtcctag aaaatatcgt ttacctacag tcataaaagt
    11881 agaactgatc aaaaaatatt tagatgacgg atttactttg tcaaaagctg cagaaaaggc
    11941 agaaatccgc caaaagaaaa tgcaccatgt ccgtaaagtt ttttcaaaag caatcaaaga
    12001 tatcgaattg atcgacgaac gctatacgat tttacaaatc ggtaacttca aagaagagaa
    12061 tgctaagatg tacatcattc atgacaacga gacagacgaa ttaacttaca agctccttcc
    12121 tgtcgatgaa aaagctgact acgaacagtt gttaaaagaa ctctaaacgc cactaatctc
    12181 ctaagtacca agctttttaa taagtagatg ctacataaat acttgaaaaa aggcctgttt
    12241 gaacataaga atatccgaac ggtgatctgc gtttctttca tatagaaagg aattctccat
    12301 cactgttcgg atattttttt atttttttcg gttactttct tctaatacat ctgttagcca
    12361 atttgcatat tctgtttccc gattgatcgc atgcgctaaa atcaggtagt gtccgtaatt
    12421 ttcatgtttt tctttttcag taggaaagat cgtttttttt cgctctaata gatgaagtcg
    12481 tttttcttca tgaaggtcac gctgctgctg aataatttct actaatgccg gatcattcac
    12541 gtctttgacg aaatacaatt tcagaacaaa ttcgtctcga ttaaccggta gttcattggt
    12601 tggtgtatga atccactctg ccaattgttc cttcccttct tctgttaatt cataaacttt
    12661 cttttccaat ttttcacctg tgatctctac ttgatgctga atgattcctt gctcttccat
    12721 ttttgcaagt tcaggataaa tctgactatg ttttgcttgc caaaattctc ctatttcatt
    12781 tttgaacgat tgattcaatt catatcctgt catcttcttc ttacttaata accctaacaa
    12841 aatatatcga agtgtatttt ttcttgccat cagttttccc tccttgacaa atctttaaat
    12901 ccattctatc ataaatgagt aatcgaaaac atgtagttta ttacatgttt taatttataa
    12961 ggagatgctt aaacatgaaa gaatttaaag aattggaaga ttttattgga actcacttta
    13021 tctatacgta tgacaatggt tgggaatatg aatggtatgc caaaaatgac catactgttg
    13081 attaccgtat ccatggcgga atggtcaaag gccgttgggt aaaagaccaa gaagctgata
    13141 tcgttaaatt gacggacggc gtattcaaaa tcacttggac agagccaaca gggacagacg
    13201 tggcattgga ctttatgcca aatgaaaaga aacttcacgg aatgatcttt ttccctaaat
    13261 gggttgaaga acatccagaa atcactgtta ctttccaaaa cgaacatatc gcagaaatgg
    13321 aagcagctcg tgaaaaatat gaaacttatc caaaattact tgttcctgaa tttgctacga
    13381 tcacttatat gggtgatgca ggtatagaca atgaagatgt tattagcgaa gaaccatacg
    13441 atggaatgcc agatgatatc cgtagcggaa aatacttcga tgaaaattat aaacgagtaa
    13501 aataaggttg tgaaagagag gttgtgaaat cagcgttctg acctgagtat tagagaaaga
    13561 aacggaaaaa tagttcctca tgttttgccg ttttttgagc taataccgag ggtcagctga
    13621 ttgaacaccg tttatcaaga ggttgtgaaa tcagcgttat gctttgagta ataagaacgc
    13681 tagttagaaa atagcttttc atattttcta ggctagtggg cttattaccg agaagcagcc
    13741 gtttgaacac cgtttatcaa gaggttgtga aatcagcgtt ttgacctgag tattagagaa
    13801 acaaatggaa aaatagttcc tcatattttg ccgttttttg agctaatacc gagggtcagc
    13861 tgattgaaca ccgtttatca aagattgtaa caactgcata ttcacacagt taaattagtg
    13921 acttcaaaat gaaataatgc tcccagcaaa aaattaggct gggagcatta tttcgttatt
    13981 ctgttcatca aaaatcagcg ttttgattcc atttcatcac aagttcctcc tcatttactg
    14041 cttcatcttt ttgctgtgtt tgaattgtaa aaccttgtga aagatagaaa tgaatagctg
    14101 attgattttt ttgatagact gtcaaagtca gtgttttatt ggtttctttt atttttttca
    14161 ttagttgcgt accgattctt tgttgccggt attccctttt cacaaagatt cctgcgataa
    14221 actgatcaat caccccagca aaaccaataa tttcttgatc tatttctgct aagtaaatca
    14281 ctgcatctgg taaagctttc ctaacagatt ctacttgatt ctcccaataa gttgatggaa
    14341 tgaattcatg agaatccaag ttagcatgca gccaaatcga tagaacagat tccagttctt
    14401 tggcagacaa ttttgttgtt gtgcggatca ttttttctct ccttacttat tcattgattt
    14461 taaaataaaa accatttttt tgaagctctt ttacaagttt tcgcccctca aaaaagtata
    14521 tcttctcttg ttttactttt ctaataccaa aagaaacagc aactttagga tcatcagctg
    14581 aaacgattcg ataagttcca ccaggcaatt gatccagctt gactttccca tgatggtcgc
    14641 tttgtactga aaacggcaca ccatttctag taagtgtttt tttcttatgt ttcaaaatga
    14701 atgaactacc atttaccggt ttccctgacg aatcactaat aataaatgaa aaatcaagca
    14761 cttttttact taatctatac tgatagatta gtcgtccgat aaacactaac agtatgacta
    14821 cagctattat cgttcctgca agaataaacc attgccgcat ggttctttcc tgatttcctt
    14881 ttccttcttc tttttttacc gattcgttat aaggaatccg atgtcctgtc actagcaaac
    14941 gatgcgtgtt gatcatatac ggcgtacatg taagcaaggt aactaagtct tctccttctg
    15001 aaatcgttag aacagacgtg tcatctggca gtaccacttg tatattttta acttgataag
    15061 ccaatttctc ccccaaaaca gtcaaaataa acgtgtctcc gttttctaaa cgatctaatt
    15121 cggtaaataa ccgtcgatta ggtaaaccgc tgtgagcaga gatcactgaa tgcgtcgaat
    15181 ttccaccaag cgggaaagag gttccttgca agacgcctgc tccattttca aggatcctac
    15241 cattgagccg atcataaagt gaaatattta attggatctt aggtatattg atacttccga
    15301 tcagccattc ctctaattgg ctggatgttg tactttcatt tctgttcttt tcattgaaag
    15361 gatcagtacc aggattgaat ccttggtcag ttaatcgttg ttttttctcc tccattttct
    15421 ttcgctgcac ttcaatttca gctgcggtcc gtttttgtac ttgttcgata cgtttttgat
    15481 caataaaact atttaaagaa tccacataaa atggatataa agcgatcagt aaccctacga
    15541 cgatacatat taataaaaac aaacgaaaca aaagtcgacg agactgtttt tgcttactct
    15601 gcttcacatg tgtcctccct tgcaagtttt tttgttcttt cttttattat atgggttcag
    15661 gaaaaaaata gaaaatgaaa gccgatgagg ttgtgaaaaa gctgtcctgc atcaagtaat
    15721 aagtacagcc caaaaaaata gctactcata ttttttgttg ggttgggctt attaccgcag
    15781 atgcaagctt ttgaacaccg tttaaggaag aggttgtgaa aactgcgtta tgctttgagt
    15841 aataagagcg ctagtcagaa aacagcttct catattttct aggctagtga gcttattacc
    15901 gagaagcagc tgattgaaca ccgtttaccg agaggtcatg aaaaagctac tttgtttcga
    15961 gtattaagaa aaattctgtt tcaaaacaag gagatgcaac aaaacttatg tcgcatcctc
    16021 tctccttaga actttcactt atgtttttct tgtaaaattc ataaatattt tcatgaattt
    16081 tctaatttct tgcctacctc tcgcatagga tatatgatga atttatctaa agtttgcaaa
    16141 ggagttcgac aattgaaaaa atatggccca tttttagcaa tttttttcat tgctttggca
    16201 tttctcatgc ctcatttatt cacacataaa ctaattttgg gtgctgatgc cccattccat
    16261 tacaatcggt tctatgatgc cgcgcaacaa attaaatatg gggatttcca ttattttcct
    16321 tccctttatg gattccagca aagtggcagg atcgtgaatg ccgtttacgg cccatttttc
    16381 gcttatttac aaggattgat cgtgttgctt gcaggtagct ggtttcatta tcagattctt
    16441 tcaaatttca ttctttatct tatttcagga ttttctatgc tgattttact gagatatgtc
    16501 aatgttcgaa attgggtaag tgtaccagta gccgttttat tcatgagtac gtattctatt
    16561 caatactggt ctatcaacca aggattcact agctggggga cttgttttta tcctttatgt
    16621 atgatcccta ttgctgattt cgtccttcat aaaaagttcc ctgttatcaa agtcgctgtc
    16681 tcagtgggac ttatgacaca gatccattta ctgtcttcga tcatgttagc tttgatgtat
    16741 atcccttttt atctttatta cttttttaac cagactgaaa aaggaaaagc actgcttcaa
    16801 ctgattaagt cggtcttgct ttattttgtt ctgacagcaa atatttgggc aggattagct
    16861 attgtttaca gtgggaataa gattcttcct ccctttgtta acagagaaat gagtcaaaag
    16921 gcgatagatc tggatggcag ttattggctc cattacccta aggcctttcc tatattgata
    16981 ttatcaggtt ttttgctttt tattttcttt caacaaaaga acaccttatt ccaaaaaatc
    17041 gtgttgtaca caagtcttgt tttctttttt ctttctacta gcttgattcc atggacgaca
    17101 ttgatcgaaa aaaatgtgcc ttttattagt ttgatccagt ttccatttcg atttttcgtg
    17161 ccatttacgc tattgcttct actttacctt gccctttcac tgaaccagtg gtctgaacaa
    17221 aagtggttcc gtcttttaag tatatttggc attcttattt tttcagcaca gacgatccag
    17281 aacttatacc agcatctgga aaagtgggaa aatgaaacat ttgtcagtcg acatacttat
    17341 ctattcgata cgccggaaga agcaaggaaa acattcttca gcaaagatat gtctaccagc
    17401 ttgttcgttt tccaaaaaac cacacctgac tatctgccaa tctataagga gacaaaagac
    17461 aataaatacg accggtataa cgaagaaata ctagaaaacg aaacaacttt tataaaaaca
    17521 catccaggcg gtcttttgac aatcaagtgg aaaaacactg gggacaaaac tgttcatatc
    17581 cctgttatcg tctacgatcg gactgtgcta cagcaaaatg gaaaaacatt gactgattat
    17641 gaagtaacag atatcgggac cccaatcgtg aagcagaaaa aaggaatcaa cgagctcacg
    17701 cttcattatc aaacgccaat ctatttctat ttcattcttt ctcttacttt gattggctgg
    17761 tttaccttgc tatgtttatt tatttatcat agatacaagc ttttacgtgc ttaggcaaaa
    17821 gggataaata catccgatca aaaaactctc caaacggtat aatcgtttgg agagttttat
    17881 tttaatcagt ttttttatgt tatatgttat attttttatt cgttcacatc taattcgatt
    17941 tccaccccgt agtgatcact gatgattgga ctatcgatcc catcaaaaat ccgttgatat
    18001 tttttaacct gcattccttc agggacgaaa ataaaatcta tacgcaattt ttctgtattt
    18061 tgttcccaac catctatttt cttttccaca gtagctgctt ttcctttttc attcgcgaca
    18121 acatacgaat cttgaagaag aaggcaactt tctcttacag tataataacc ttgagagtct
    18181 actggattat tgaaatcacc taggaaaaaa agtggtacgc gagtttccaa aaagtagttt
    18241 tccaagtttt tccactcaaa ataaaagcca ctttttttat ctgtccacca agaaaagtgg
    18301 cagctcgaga caaaaatgtc ctgatcatct atctttgttt tcccaatcaa gatcttgcgt
    18361 gtctgataat cttctttctt ttgagattgg gagacggtaa ggacctgact ttccaatgtt
    18421 tttttcgtaa gcagtgcatt tccttcttca tatttatcat aaccgatgtg gctcatttcc
    18481 caactccaaa aatactcttt tccgtgttcc tttaaaagct gaactaaacg gtaggcaaaa
    18541 ttatctttac ggataggtac ttgattcttt acctgacaga acttcattag cttcgcaggt
    18601 tctacttctt cagattccag taattggttg atttcttgta aagcaatcac ttcataatct
    18661 tctaacagga tcttatctgc taattcttgc aattttttct ctggttctgg ctcaagccag
    18721 ctatgcgtgt tgagtgtcat gaatttcatt ggttttctcc ttgtcttcat ttcgaaacaa
    18781 aagggttgtg acaaaatttt tgtcacaacc tctccagcaa atagtgttct cctgctaact
    18841 cctcagtaat aagctcaatg gaccaggagc tattttttgt ttggctgttt ttatcacttg
    18901 gtgcagggca gctttttcac gacctctcca ctaaacagta ttcaaactgc tgcttctcgg
    18961 taataagccc aaccaaacga aaaataggag gagctatttt tgtttggctg ttcttatgac
    19021 ttgatgcagg acagcttttt cacaacctct ccttacattt taatggagcc gatcacttta
    19081 ccagctgtca ctgtacctaa ttgcttgatc actacttctt ctactttatc gtttgtaaat
    19141 acgatcatga tcgttggatc tttttcttcg ttttggatca catcaagatc catctcagct
    19201 aatttcgttc ctgttcctac ttcttggcct tctttgacag caatctcaaa agctggctga
    19261 ttcatttgga ctgtatcgat tcccatatgg atcaaaactt cgataccatc tttcgtttcc
    19321 agcccaattg catgtttgct agggaaaata cttgtcactt ttccagcgat cggcgcaaca
    19381 acttctcttg atgttggaat aacagcaaag ccgtcaccca tcattttttg tgagaatacc
    19441 ggatctttta cttcagataa aggaatcact tctccagaag ctgcggaaac tagttcttgt
    19501 tctataccta agtattgctt actacctgct tgtccatttc ctgtagaaac catttctggc
    19561 tctggaatct ctgctcctga ttgcagcaga tcttcgatat ctgatttcaa gacatctgct
    19621 tttggtccat aaactgcttg gactccattg tctttgacga tcaatcccat cgcccctgct
    19681 cgtttccagg cttcttcagt ccctactttt tctcgatcat ttacacttac gcgtaaccga
    19741 gtcatacaag catcgacatc tttgatattt tcttttcctc ccagcaaatg aatgatctgg
    19801 accacttgct gatctgctgt tcctgttccg ctgttagata caacggtttc ttcactgtca
    19861 ttgtcgtaat ttccgttacg tcccggtgta gcaaagttga attttttgat caagaaattt
    19921 gctaagaaat acgtaacaac accaaaagcg atcacacaca acacgaagtt gataagatct
    19981 ccacctagac ccgctttgat agctaacggt gtccgagtca gcaattcgat attcccaaat
    20041 gaatggaccc gcaatggcaa gatatctgcc atcgcaaatg cagccccttg gatcactgaa
    20101 taaatgacat ataatggcac agcagcaaac ataaacatga actccaatgg ttctgttaca
    20161 cctgttagaa agacagccaa cgctgctgaa aaatacattg atttatatct tgctttctta
    20221 tcaggatcga cattacgata catcgcaaaa gccattccca tcaagatacc tgaagaacca
    20281 atcatttgtc ctactttgaa acgagccggc gtccagttat gcaacacaaa ctcatattgc
    20341 gacatatccc ctgcaccttt aagattcact aaatcagtgg cccaagccaa ccataacgga
    20401 tcttgcccaa atacttgtgt ccccgcctgt gcgcctgata aaatctcata cgatccgcca
    20461 agctgtgtat agttgatagg aatcgtcagc atatgatgca atccaaatgg caatagtaaa
    20521 cgttctaatg ttccaaataa aaacggtgct agaatcggtg cgctttcttg tgattctgca
    20581 atccataaac caaaattatt gattccagcc tgtacgtttg gccacaccac agcaagaatc
    20641 aatgccacga ttgttgacca taagataaca acaaacggaa caaagcgctt tccattgaaa
    20701 aatgacaaag catccggcaa ttttctataa ttatagtact tattataagc cattgccccg
    20761 acaaatccag cgatgatccc tacgaatact cccatattca aagctggtgc ttccaataca
    20821 cttgtaaaaa atcctttaac catgatcttc gtaccgaaca gagtgtgggt aaatgcatct
    20881 tcattcgcaa gcatgtcact tgtgacacca aagatagccc cagtgatccg attgattaaa
    20941 ataaatgaga tcccagctgc aaatgctccg cctgctcttt cttttgccca acttcctcct
    21001 attgctaaag caaataaaat atgaagatta ccaataattg cccaaccaat attttcaatt
    21061 actccgcccg ttgtgacaag cggcgtccaa tcaggattga tcaagggaat tgatttccct
    21121 atactgatca tcaacccagc tgccggcata actgctacca caaccattaa agcctttcca
    21181 aatttttgcc aaaactcaaa actaaatatc tttttcattc cgtttagctc ctttacgcaa
    21241 tcggtttcgt taaacgtatt atagcgtgcg ttttcttttt tgtaaatgct ttatttttta
    21301 tttttctgtc tataccattc tataaaacct ataatacgtg tgttcccttc tataattttc
    21361 tcctaaaaat ttattttttt aagctttttt ctatgaaata ttttcttttg tctattgact
    21421 tgtgcaatcg gttgcgttta ttatgaacgt ataaaacagc tcattaggag gagaaaaatg
    21481 aaacagatca aacgactatt tgctattgat ccatggaaaa tcagcacgac acaattagat
    21541 aaaaaaaatc ttcgactgca agaatcttta acaagtattg gaaacggtta tatggggatg
    21601 cgcgggaact ttgaagaaac ttactcaggg gaccaccatc aaggtactta tttggcgggt
    21661 gtctggtatc ccgataagac acgtgtcggc tggtggaaaa atggttatcc agaatacttt
    21721 ggaaaagtga tcaacgcctt gaactttatc gcgatagata tttcgatcaa cgatcagctg
    21781 atcgacttgg cttctttgga accagaagac ttttactggg aattagatat gaaaaatggt
    21841 gtattatctc gtacattcac aatcactact tctactaata aagtacgcat ttccttcgaa
    21901 cgttttctga gcattgtcaa aaaagaatca gcttatatct atttaactat agaaatgctt
    21961 gaaggtagcg gaacagtcaa actcgtatca aaattggatg gaaatgttca aaacgaagac
    22021 agcaactatg atgaacattt ttggaatgag attgatcgtg gaactataga agaaatcggc
    22081 tatttgacga ctaaaactat tccaaacaac tttgggattg aacaatttac tgttactgca
    22141 gcaatgcgcc atttcgttaa tggaaaatca gtcatccctg actatcacga agatacttta
    22201 gcattacatg gttgcttgtc tgcagaatta tctcaaggtg agacatggtc agtggaaaaa
    22261 gaaattatcg ttcttactag tagagatatt ccagaagcgc agcaagcaga aaaagctgtc
    22321 tcagaattaa cgaaccagac aaacaattat atccaagcaa aagaagagca aacagcggct
    22381 tgggcagaac gatggaagct tgccgatgtg gtcatcgaag gagacgaaga agcacaacaa
    22441 ggtattcgat tcaatttgtt ccaactattc tctacttact acggcgaaga tgaacgattg
    22501 aatatcggcc ccaaaggatt cacaggagaa aaatacggcg gagccactta ctgggataca
    22561 gaagcgtatg ctgtccctct ttatcttgct ttagccaaac ctgaagtaac taagaatctg
    22621 ctgaaatatc ggcataatca actgccgcaa gcaatccaca acgctcagca acaaggatta
    22681 aaaggcgcgc tttatcctat ggtcaccttc actggtgtcg aatgccataa cgagtgggaa
    22741 atcacattcg aggagattca ccgaaatggc gcgattgcct atgcaatcta caattatacg
    22801 aattacaccg gcgatacgga atatttgaaa aatgaaggac tggaagtttt agcagaaatc
    22861 gcacgcttct gggcagaccg tgtccatttt tcaaaacgag caaataaata catgatccac
    22921 ggtgtgaccg gcccaaacga atatgagaac aatatcaata acaattggta tacaaatacg
    22981 atcgcagctt gggtcttgcg ttacacgcga gaaaattatt taacttatca aaaagaagct
    23041 gctatcacta tcagcgaaga agaactagcc aaatggaaag atatcgcaga gaacatgtac
    23101 ttcccagttg acgaagaatt aggaatcttt gtccagcatg acacattctt agataaagat
    23161 ctcatgcctg tatccgatat tcccacaagc gatcttcctc tgaatcagca ttggtcttgg
    23221 gacaaaatcc ttagatcttg cttcatcaag caggcagatg tactacaggg gatttatttc
    23281 ttcaatgata cttttacaaa agaagaaaag caaaaaaact ttgatttcta tgaaccaatg
    23341 actgtccatg agtcttccct ttcgccaagt atccattcga ttttagcagc agaattagga
    23401 atggaaagca aagctgtaga aatgtaccag cgaacagctc gcttggattt ggataattac
    23461 aataacgaca cagaagacgg cttgcacatc acttccatga ctggtagctg gttgtcgatt
    23521 gtccaaggat ttgctcaaat gaaaactgcc catgatcagt tgagattcgc accatttctg
    23581 ccaaaaactt ggaaagctta ttctttccat atcaattatc gcggacgttt attgttcatc
    23641 tcagtcacag atgaacaagt tactttcgag ttattagaag gttttcctct agaaatttac
    23701 gtctatgaac agtcttattc tttagcggat cgtctagaaa taacgctaag aaaggaggaa
    23761 gcaaatgttt aaaggggtat tgtttgattt agacggtgtc attaccgata ctgctgaatt
    23821 ccattatcgt gcttggcgaa agcttggtca ggaaatcggc attacgatcg accacacatt
    23881 caacgaacag ctaaaaggcg tcagccgaga agactcactc tctttgcttt tagcttatgg
    23941 cggaaaagaa cactcttttt caaaggaaga atttgcagaa ttagcgaaaa gaaaaaacga
    24001 ttattattta gagatgatcc agacgatcga accaaaagat gtcttccccg gggtcgtgcc
    24061 tttactggat tctttaaaag aagaaaaaat caagattgct cttgcttctg caagtaaaaa
    24121 cgggccattc ctacttgaaa aaatgggtct tacaccttac tttgatgcta ttgccgaccc
    24181 ggcagaagca acaaacggaa aacctgcgcc agatatcttc ctccttgcag caaaagctgt
    24241 cggactaaaa gcagaagaat gtatcgggat cgaagacgcg caagccggta tccaagccat
    24301 cctctcaagt ggcgctcaac caattggcgt gggacgtgct gaagaccttg gagaggagat
    24361 cccgcttgtc cctgatacga cttttcttac gatcgattac ttgaaagaaa agtgtcatga
    24421 ccatggataa tccagagatc tccgtcgtac cagttccaga gactgaaata aagaaaatca
    24481 cattgaagaa cgactactta gaagtcgttc ttctcaatta cggtgcccgc ctgcaccaaa
    24541 tcttcgcacc agacaaagaa ggaaaatcag aaaatgtttt attgtcttac gatcgtttcg
    24601 aagatgttct aacagacaaa agcttttttg gagcaactgt cggacctgta gccggaagga
    24661 tccgggatgc ttcttggggt gagcatagat tggagaaaaa ctgcggatca caccatatcc
    24721 acggtggcac gaaaggctgg tctttccaat tttgggaagt agaagttttc aaaactccat
    24781 catcaatagg tgtggtcttt tacttaaaag atgaattttc tacctatcca ggaccaatca
    24841 ctgctacgat cacttaccgt ttaacaaaaa acgaattaga gatgacaacc tctgctagta
    24901 gccttgttga aaccatctgc aatccgacca accatgccta cttcaacctt tcaggaaatg
    24961 gaacaagaga catctatgaa catcagttaa cggtcttttt agatggtgtg cttgaattag
    25021 atcaagaaaa actgcctact ggaaattgga caaaaaaaga aaaactgcca atcgacttta
    25081 gaaaatcccc aacactgcaa gagatccttg catgttatcc agatggacta gatgatgtat
    25141 ttcttttgca tcatcctagg ctttccaaaa cttccttaca attattcgag aagcattctg
    25201 gtcgccaaat gactatagca acttctaata aaagtatggt attattttct acgactggat
    25261 tcgaagctga tttctctgtc aatggaaaac agatgcattc aaactacgga ttagcaatcg
    25321 aaccgcaaga aatccctgat atcgttcatt tccccaaatt tggatcgatc aacctccatc
    25381 ctggtcaaga acggatcagt cagacgattt atcggttctc tgcacaataa caagagaaag
    25441 gtgtttttat catggctatt accgtaaaag atgtggcgaa aaaagccggt gttgccacct
    25501 cgaccgtctc tcgtgtcatc aacgatcatc ctagtatttc cgagacaaca aagaagaaag
    25561 tccgaaaaat tatggatgaa ctaggttatg ttcctaacat gactgcccgc aatctaggaa
    25621 aacgcatttc cagtgccatc ggcgtaatct taccccctct tgattcaaaa gagcggatcg
    25681 gtaacccatt ttatcttgag atcatggaag caatcaacga agaagcgcgg aattatgata
    25741 tgactacagc aatcgctact gctaaaaatt ttgaattgct gttagagaac gtccagcgta
    25801 tgcatttaca aaaacaagta gatggtttta ttctagtgta ttcagatagt caagatcctg
    25861 tcattgatta tttataccaa aaccacatcc ctttcacttt gatcggacag ccttatcatc
    25921 atgagaacga aatcgtttat gtagataatg ataaccagct tctaggtaaa caagcaacag
    25981 aatttttgat ccagaatgga cataagaata tcctgtttgt gacaaacacg actcatgaaa
    26041 gtttgtattt cgaacgatat ttcggttatc aaaaagcaat gatgatggct ggtcttcctg
    26101 cgttatccag tgtagtacta gagcatccgg aggattatct gtcctttgag aaaacgttga
    26161 aagaaaacga agccacagca ttggtggtca ttgacgatat cttcgccctt cgcacgatgc
    26221 agttggcgca aatgtacggc tatgtcattc cagagacact ctccgtcatt agcttcaaca
    26281 actcgatctt ctcaacatta acacaccctt accttacaag tatcgatatc gatatctccg
    26341 aattaggacg tgtcgctatg caaaagctgc gcgaattgat ccatgaagaa ttgagcagtg
    26401 gcgtacgttt ggtcatccct catcgcttga tcaaaagaga gacagtcgtt cctttagaaa
    26461 acccatacta aatagctgac tcactaaata cgctgaccat ttatcctatg gtcagctttt
    26521 tatgtaaaag aagatactac ttctaacaca ttgtttatct gtaaaaaaga gcgtgggaca
    26581 aaagtaaaga acacttttgt ctcacgctct aaacacgtat aaacggcgga agcagaagca
    26641 actccttcgg aaataagctg aaattcacaa aaatttaaaa agcaattttc ttgagtttct
    26701 tcttatttct tggagttaaa cgcttctgtc ccagcctctt ttttccttcc ttcaattttt
    26761 tacacgtcga cgattcaata aaaaaccgcc taatattgta aagacaggtg caagaatcga
    26821 gaaaaagacg aatggcaagt aatgcaacgc gctaactttc atcgttccca taatgaatgt
    26881 gccgctgact ccccacggta cgagagaatt gacggtcgca cctgaatccg ctagtgttcg
    26941 tgtcaaatag gtttttgata agccaacgtc atcaaaagaa cgcttgaatg tttctcctgg
    27001 aagaatgatg gacagatact gttctccaac gatcagattg attccgatac agctcaaggc
    27061 agtaaacccg actaaacgag aaggtcggtc catcttttct tttaattctt gaacgatggt
    27121 atcaacgatc agatatttga tcagtaaccc acctaatgcc aatgcaagta aaatcaaact
    27181 agcagaacct aacatactca tgatcccgcc tcgagaaagc agttcatcta gctttggatc
    27241 aggtgtattt gcgacataac cattcatcaa gatgtctgct gcttttccga ccgtcaaact
    27301 tttctcatgg ataaaagaaa gaacgagagc agctgtcgat cccgctagta atgtagggat
    27361 agccggaatt tttttgattg cccccccaaa taaaataact agtggaatca atgtccaagg
    27421 agagatccaa aagctcgctt tcatgttttg gatcatttca ttgatactag ctagatcggc
    27481 tgaatgactg ttatgtccta atatcgtata aaaaatcaac gaaaggacga atgctgggaa
    27541 atccgtataa cgcataccag cgatatgttc gaatagatta actcctccaa tacccgctgc
    27601 caagttggtc gtatctgata aaggcgagca gttattccct aaaaaggcac cggaaacgac
    27661 tgcgcctgta gtcattgcat cattgaatcc aagtaaatga ccgatcccca aaaaagcaat
    27721 tccaatcgtc gaaatcgttg taaatgaact gccgacagtc actccgacaa ttgcacaaac
    27781 aagaaatgct gtcggtaaaa agaaacgcac agaaacaagt ttaaatccat aaaccataat
    27841 cgtagggatg gttccagata aaatccatga agcaactaag acaccaatca aaagaaaaat
    27901 caggattgga atgatccctg gagtgattcc tttgatgatc ccgtcatgga tctcatccca
    27961 tgtgaacttt ttaaatctgc cataaaacaa caaacacatg aaagctgcaa ggatcgggat
    28021 atgtggcgag agctggaatc cgatgatcag tactcctagg agtgccaaca acacgatcaa
    28081 tacagcgata ctttctttta aagaaatatt tttattcatc gtcgatctcc tatttcaaat
    28141 gaaaaggccc tgccatgaaa aaactattca cttggcagga cctcctacta aatcagcgac
    28201 aaaacgtcgg ctgaaagact tatttttttt atttttcttc taaacttttt tctacctcaa
    28261 tcccccaaga atctttcttg aagtgattga tgatcaatgg aataacgaac atgaccactg
    28321 tgatagctgc tactaataat gcatcactca atgtcgctgt acctaatgta gcaaatacga
    28381 caacgatgat agagaagatc agcatgcagc tgactaccca agctaatccg tttccgtttt
    28441 taccaattcg gtaaggacgt tcgaattctg gatgtttctt acgcatttta agcaatgaaa
    28501 cagcaatcaa aatgtacacg atacaataaa taacagttgt agcatttgta agtgtcaaga
    28561 atacagcact tacgtcatcc attacaccat aaagcaaagc aaataatgaa ataacgattg
    28621 attgtgttag aacgacattt ctagatacgc cgtatttgtt ttctttatgg aatccgaatt
    28681 ttggtggaag gaatccttca cgagctactt ggatgattgt ttttgatggt ccagtaaccc
    28741 aagctgataa ttgtaaaagc acaccaataa ataccatgaa gctgaagata tttccgatca
    28801 cttctggtaa gcctaggatt tgacagtcaa tcaagatagg ctgcgtgata tttgctaatt
    28861 ccatttttcc atctggtacg acatttgcta caagcatcgc attgatgaca ttcaataaaa
    28921 ctaatccgat caaagcgata aatacgcctt ttgtataatt ttttgttgcg tctttcaaac
    28981 gaggcatgta aacagaactc atttcgatcc caacaaaaat aaatgtgata ccagctaagt
    29041 atttcaatga atcaatatgt tccaagtcag ggaaagcttt tgaccatgaa aagtcaccta
    29101 gatagccgtt tgagactagt ccgactttga aggctgccaa aacacctaaa acgaacatga
    29161 tgactactgg gatatatacc cctagccata caccgatgtt cccgccgact tttgccatat
    29221 cgaatttcaa gttcaagatc gtgatgatcc agtagataac taaaatacaa cctaaaacga
    29281 accagtgatt attgccaagt tctacattac caaatgtgtt tcctaacaat ggtcctaaag
    29341 tagaagcgac cattaccata cctgggaaca tttgcaccca taatagccat gcaacgacaa
    29401 atccccattt gctgcccagt gctgttttta cccataactg aggaccgcct tcttgaggaa
    29461 gcatcgtgga aagctcaccg gaaaccattg atatcggacc ggcaaaaaac aaaactgcaa
    29521 aaattgaata aaaaatcaat gtccaaccga ctgcggctaa cgtaggaata ctccgaaccg
    29581 ttgcacacag tgccattgtc agaccgataa aggtcccaag acccatttta cttacttcag
    29641 atttactcac cgttgacact ctccttgatt ttttggataa aataacggtg tctttgaagt
    29701 agtttcagca ataaacgaaa caattcagcg aatatttgag gggattcttt gagtctattg
    29761 cttgaagggt ttcttcaaag acaacctcaa aacaaaggga tcgaagagct taggtctccc
    29821 cttgctcttc agatccaaac atgcgtcaga aacagttagt attcaagctg ttttctaaca
    29881 tgattacctt gcttattttg cttcgcttgc caataattgg tctgcgtaga ttttttccaa
    29941 tttttcttga agagctgctt tgattttttc agcatattct tcaaagtgtt cttctttgtt
    30001 catgtatggt gtcattacgc tggcacgcaa tacagttact tttccggcac ggttccattc
    30061 ttcatctgag aatcctagtt ggttcacaaa ttgtaatggg ctgttgccat agtctgggat
    30121 agcaaagtca gtatgtgaag ttaagaactc attaccgtaa atgcttcctt tcacgtatga
    30181 agaataatca tatacgtcat ggttcaattt attcattgca actaagtcat cgttgccttt
    30241 ttctttgaat acatagtcta ccatattgaa atctggataa gttaatggat gaacttcgat
    30301 ttctttgtct cctactttaa atgacaagtc attcaagaag ttgtagaaac gatgtgcacc
    30361 ttcgattgat gcacccatta atttaccata tcctgtgaca ttcaaaggta agacatggtg
    30421 tgctgcccaa acgctggctg ctgttgcccc tgcttttgaa ccttccaaga tataagcccc
    30481 aagtaatgct gggatatctg cgcctttttc aaatacatat gtcgcaaagt aagagataac
    30541 gtctctcatg cggatatctt tgatgacgat accaccggca gaatatggaa catagcccat
    30601 tttatgtggg tcaatcgtta cagattctgc ttcttcgatt gctttatatg cactgtgtac
    30661 ttcttctaag atataatctt tgttttctgt aaagacattg tatttgtaat gaacatcttt
    30721 taaatcttca aatggaatga agttgttgtc ttcgtctaag aaaatcgcac ggccgtatcc
    30781 gccataagca gcatctacgt gtaagtagaa gtaaataccg tctttttcta atacgcgacg
    30841 caatgcaacg attttatcga taccgtcgat tgctccttct tctgttgatc ctacaacacc
    30901 gactacacct aggattggtg ttttttcagc tgctagacca cgaacgattt tttcaagttc
    30961 gttgatgtcc atgcgatagt tgtggtcaac tggcactgga atcacttggt ctaaaccgat
    31021 accgatgatg tcggcagctt ttagccatga atagtgttta gtttgtggaa ccaaccattt
    31081 tcctaatttt tctaaatgtt ttccgctacg tgctgagtga gctttgattt cgtcgatttt
    31141 ttctgggacg ctgtctaaca gattcatgat ttcttctgtt gacaagttca ttaattccca
    31201 atcgcttttt ccagcaacta gttcaggtgt cacttctttc attgcaagcg gcaatgattt
    31261 gatgttgcgg gcataccaaa gtccttctaa gtttgctaaa gaaccatcag caacgatgtg
    31321 gccccagcca tctttatagc tcattaattt tgcaaattcc attcctactt cttcttccat
    31381 ttggcttgtt gcaggagaag attcataggc aacgttatta ccattccata gcatagcaaa
    31441 attgtacgct agtaaagatg gcatcaatgt ttcagagttc atatgacccc agtaacgtcc
    31501 ggcattatgc caaggaacgg aatgggtacg catccgcgct gaaatttctg atagaacatc
    31561 ctttgtttta ttcacagtgt gttcaaaact agcactgctt ttttcttctg gcgtaataat
    31621 aggcatgtct tgaggcatgt agttttgacg ccagcctaaa tgttcatcca ccaattcatt
    31681 caacaaagct ttatagattt ggccgttctc agctttgtcc ccaatgaata gggcatttag
    31741 gtttagatct ttcgacaatg attcactcat agtgaaaccc tcctaaaaaa atatttaaat
    31801 tttgtcgagc cctacattac acctgttctt ctattctgta aactaagttt tggcctgatt
    31861 tttaatcata ttttaatatg tatcaattat ccgaattgtt cacacttttg aaataattaa
    31921 aaaaaacgag ggaatttaca aatgtaaact ccctcgcaaa gattgtatta acgacaacca
    31981 gcgttctatt ttaggatggg tttcaaaatt ggaatgttgt aatagtattt ccctttttca
    32041 aggtaaaaac ttactaaatt ctaatcaatt actttggcca aaaagtaatt tttcttacct
    32101 tttcgaataa ccacaaactt accatcgaat tcatcggatg gatttatgac aaaatttaaa
    32161 tcggtaacgc ggtcaccgtt aatacttata gctccgtttg aaacatcttc acgtgcttga
    32221 cgtttagatg gttcaatttt tgtggaaaca agcagttcca cgatattttc aggtgtgctt
    32281 gaaatttcaa cgttcggcat tttaccgaat ccttgcgcga tttcttccgc atttagttct
    32341 ttaatatttc cagagaacaa tgcttgcgtt attttttgcg cttctttcaa gtcttcttca
    32401 ctatgcacaa atcttgtaac ttcttctgcc aaacggcgtt gtgcttcacg tttctcaggt
    32461 tctgtctcta cttttttcgc taagtcttcg atttcttctt gagacaagaa agtaaagaat
    32521 ttcaagtatt tcactacatc gcgatcatct tgattcaacc agaattggta gaactcaaat
    32581 ggtgaagttt tcttcggatc aagccaaacg gcaccgcctg cagtttttcc gaatttcgtt
    32641 ccgtcagcct tcagcatcaa tgggatcgtt aaaccgaacg cttctacttc tggtccttct
    32701 aattttcgga ttaggtctaa tccagctgtg atatttcccc attggtctgc tccaccgatt
    32761 tgcaattgca catcgtatgt tttatgcaga tgcaaaaagt cgatagattg cagaatttgg
    32821 taagtaaact ctgtaaatga gattcctact tccaaacggc tggcaacgat atcttttgct
    32881 aacatcgtgt tgatgttgaa gtttttaccg tagtctctca aaaattcaag taaagagatt
    32941 tttgacaacc agtcgtagtt gttcacaaat gtgatatttg catctttacc aaacaatttt
    33001 ctcatttgat tggaaagtgc gtccacgtta tgttgcactt gttccatcgt ttgcaacaca
    33061 cgttctgttt tgcggccgct tgggtcgccg atcgttcctg ttccgccgcc aatcagaata
    33121 tatggatgat ggcctgccaa ttggaatctt ttcatcatca taaatggaat caaatgtccg
    33181 atatgcatcg aatcaccagt tggatcaacg ccgcaataaa gtgaaatgct cttttcttca
    33241 acgagctcgc gaagtctttc ttcgtttgtt tgctgattaa tggcgtcgcg ccaagttaat
    33301 tcatcaataa tattcatttt actgacctcc gtgtagtttt tttatgaaag caggaacctg
    33361 ctagcatgtg taattaatta tttcttcagg ataggatagg aaataaaaaa agtcccttgg
    33421 catataagcc aagggacgaa atctcgcggt accacccaat ttgtatgtct agatatccaa
    33481 catacctctc gaacatttaa cgctgtcgcg tggcaatcac tgccaaagac tcctgaaatg
    33541 taattcgctt taaagcatgt actggtttgc agcgaccacc agctttctga aacattggct
    33601 ttaacaacta cttcgtttcg atcttcgtct tgttattatt aatatgtgtc tatcatagag
    33661 cgcaactttt tctttgtcaa cagaaaaagg atattttttt taaattcctc attttttctt
    33721 ttttgaatcc gctctaattc cgaacatttt attttcaatt attgttaaaa gaaataactt
    33781 tttctgtatt ttttctgcaa tttatcgaga agtcacttta ctttgcctct tattctttct
    33841 tcgttacaat aaggaatggg acaagtaaaa aaatgaaaaa gcaagggacg aggtatatgg
    33901 aaaaaaagca aaaaattatt ttaggagcca cttctgcttt aactatcgtt attttaattc
    33961 taagtttgtt ttttgtcatc cgatcatcta caaaaaaaga accaatcatt cctgaaaaaa
    34021 aaataacaga aaaagcaaaa gaaccgacag atgataagca acaactagtt actcaagcaa
    34081 aaaatctggc tcaaagctac gattatgacc aagcgattgc gttattggaa aaagatagta
    34141 agaacggatc tactgaaaca caacagcttc ttcaaacact aaaaaaggag aaagaacaac
    34201 tgatcgactg gtctgatcct actcagatct ctcatgtctt tttccacagc ttgatcgtgg
    34261 acccgcaaaa agccttccac tctcaacagg cacaaggata caaagattat atggtgactg
    34321 ttgaagaatt caaccgttcg atcgaacaac tttatcaaaa tgattttgtt ttagttaatt
    34381 taaatgat
//
LOCUS       DACDKM010000018        33649 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20834_2016
            SAMN12640370-rid9891663.denovo.018, whole genome shotgun sequence.
ACCESSION   DACDKM010000018 DACDKM010000000
VERSION     DACDKM010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640370
            Sequence Read Archive: SRR10040891
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 33649)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33649)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742835.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742835.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:04:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,012
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 29 of 75
            Pseudo Genes (incomplete)         :: 33 of 75
            Pseudo Genes (internal stop)      :: 19 of 75
            Pseudo Genes (multiple problems)  :: 5 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33649
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640370-rid9891663.denovo.018"
                     /isolate="E20834_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            131..1792
                     /locus_tag="IV334_000883"
     CDS             131..1792
                     /locus_tag="IV334_000883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HAP8942974.1"
                     /translation="MKKKFTFGLVAVCGLVLAGCYGGSSATSSSSAKSGSADGGGVFN
                     LVVPQEMPTADLSVATDTISFTALNNVYEGIYRLDEDSKPQPAGASELAEVSEDGLTY
                     KIKLREDAKWSNGDPVTAADYVYGWQRTVDPATASEYAYLYAPVENAEDITAGKKDKS
                     ELGIKAVGDYELEIKLTKQTPYFQYLLAFPSFFPQKQSVVEENGDAYASASDKAVYNG
                     PFTLEDFDGPGTDTEWTYKKNDHYWDKDTVKLSEIKVSVVKESSTALNLFKDGQADDV
                     ILSGELAQQNANDPAYTSVKEARTSYIEFNQREKNSPFNNVNLRKAISYSIDREALVK
                     QVLGDGSVVSTGLIPSDMVKSPTTNEDFAKEAGKLVSYDKDKAKEYWEKAKKELGVDS
                     LEFDLMASDDDSSKKVIEYVQNSIQENLDGVTVKPTPVPFSVRLDRSTSGDFDAVLGG
                     WAADYADPSSFTDLFVTGNSYNRGRWSNAEYDEAVKAASSTDAGDEQARWDDFQKAIK
                     IISDDMGVVPVYQKAEGHLVNEKIKGIVHHAAGASWDYKWTYVEE"
     gene            complement(1862..2209)
                     /locus_tag="IV334_000884"
     CDS             complement(1862..2209)
                     /locus_tag="IV334_000884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3899 domain-containing protein"
                     /protein_id="HAP8942975.1"
                     /translation="MKKKSIPYAVAFLLILVILIKNLINHSFTLIQLSNDLFLWSLPF
                     LIIGGFLWVFSSGFFDHFQRSVHLARTRNRKKKPEFSSLSSASYGMYSFWLIIAGILI
                     ALSAIFMLFSLLG"
     gene            2293..3234
                     /locus_tag="IV334_000885"
     CDS             2293..3234
                     /locus_tag="IV334_000885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP8942976.1"
                     /translation="MNSYIKYVLKRVFFMIITLWLIATITFFLMQLLPGTPYTNQERL
                     SPETIEMLNKQVGLDKPVIVQYGIYLSNLLQGDFGISFQFKNQPVAHLLAGRIGPSLQ
                     LGLQAIIFGTVLGTILGTISAMKQNTWADTSSTLVAILGRSIPNFVFAVLLQYIFAIK
                     LQVLPIAKWDGFVYTILPTIALAMSPLADSARFIRTEMVEVLHSDYVELAKAKGLSRW
                     EIAFKHGLRNSLIPLMTLLGPLAVALMTGSLVVENIFAIPGIGEQFVKSITTNDYPTI
                     MAVTILYSFMLIFVILIVDLLYGLVDPRIRVSEGSRG"
     gene            3235..4278
                     /locus_tag="IV334_000886"
     CDS             3235..4278
                     /locus_tag="IV334_000886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP8942977.1"
                     /translation="MEMIPKRYETVADIPADEFLPLQKNTEEEREQITAPSLNFIQDS
                     WRRLKKNKAAVISMVLLLVIILISVITIFVSPHDPTAQNVAYINLPPRIPGINIDGLN
                     GKAMVGGELVDKYAQANVPSNVNFFLGTDGLGRDVLSRLFMGMRISLLIAFIAALLDV
                     TIGVAYGLVSGLLGGRVDNVMQRFLEILSGIPNLVVMILMLVVFEPGIFSIVAAMAIT
                     NWIPMARIVRAQTMKLKDQEYVLAGLTLGESRMKIAFKHILPNISSVIIIQMMFSIPS
                     AIFFEAFLSFIGLGLTPPSASLGTMLSEGYKTFLYLPYLLWIPAVTLSVIMIGFNLLA
                     DGLRDAFDPKMKE"
     gene            4284..5354
                     /locus_tag="IV334_000887"
     CDS             4284..5354
                     /locus_tag="IV334_000887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP8942978.1"
                     /translation="MTKILEVKDLEISFDTYAGKVRAIRGVNFHLNKGETLAIVGESG
                     SGKSVTTRSIMRLLSSNANIDAGQILFKGNDIVGKSEREMQKIRGKEIAMIFQDPMTS
                     LDPTMPIGKQVAESLRKHNKVSKKEGQKAALELLKLVGIPEAEKRINSYPHQFSGGQR
                     QRIVIAIALVCYPEILIADEPTTALDVTIQAQILELLKEIQTKIDTSIIFITHDLGVV
                     ANVADRVAVMYGGRIVEVGTSEEIFYNPQHPYTWGLLGSMPTLDSANDRLYAIPGSPP
                     DLLNPPKGDAFYPRNEFAMKIDAEQEPPFFELSKTHKAATWLLAPQAPKVTPPEEIQR
                     RWAIFKEKQKHTYGQGGIADAK"
     gene            5344..6297
                     /locus_tag="IV334_000888"
     CDS             5344..6297
                     /locus_tag="IV334_000888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAP8942979.1"
                     /translation="MPNKLLEVKGLKQYFNVGKKNEVHAVNDISFHIYEGETFGLVGE
                     SGSGKSTTGRTIIRLNEPTDGEILFDGQDITKIKDKKGLTKFRHDVQMIFQDPYASLN
                     PRMKVRDIIAEGIDVNGLAKSSEERAKKVDDLLRTVGLNPSHGTRYPHEFSGGQRQRI
                     GIARALAVKPRFIICDEPISALDVSIQAQVVNLLQDLQKEHQLTYLFIAHDLSMVKHI
                     SDRIGVMHNGLLLEMGTSDEIYNHGVHPYTESLLSAIPLPDPDHERKRRRIKYQPEPD
                     DGQVRQLREIAPEHFVYATEQEVPYYAKKLKRQKESLLVAN"
     gene            6309..7319
                     /locus_tag="IV334_000889"
     CDS             6309..7319
                     /locus_tag="IV334_000889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAP8942980.1"
                     /translation="MLKAFKFRMYPTEEQKQQLIRTFGCARFTYNHLLKKRQKSWQQT
                     GVANFSLTPATLKKEYPFLKEVDSLALANAQLNLDRAFRNYFKGRASFPKLKTKKSMW
                     QSYTTNNQTRTIYLENGYLKLPKQKELIKINQHRPVEGSIRSATISARYNEEFYVALL
                     CDVSPVKKESLAKWIGIAYHPKTLIQTSRPLEVTLPKFHQTEEKLQHAQRKLNVKVRS
                     AHHRKIRLDQASNYQKQKRKVMDLYLKQKNQREDYLEQLSGKLVKQYDYLFVESFPKE
                     EAHADFSIHDWHKLITKLRYKSQWYNKKFLLINTDGAEESNSVRKSQVVEKMGRHSVI
                     KG"
     gene            complement(7604..8425)
                     /locus_tag="IV334_000890"
     CDS             complement(7604..8425)
                     /locus_tag="IV334_000890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HAP8942981.1"
                     /translation="MNRFASKEEEKLYYEHKASEEEFLNWYQKQERPEYDKPSLTVDI
                     VLMCYNKEEDQLKVLLIQRKGHPYRNSWALPGGFVQKDESTGESVLRETKEETGVVIS
                     KENIEQLHTFSTPNRDPRGWVVTVSYLAFIGEEPLIAGDDAKEVRWFTLERHSNKIHL
                     SSGEVAISLDLVTGESSGKDTLAFDHSQIILKAFNRVVNKMEHEPQVLQVLGKDFTIT
                     EARKVFAKFLGIDYKTIDHSNFKKAMLQYFDEIGERPVGIGRPSKIYQLKPGHHE"
     gene            complement(8522..8800)
                     /locus_tag="IV334_000891"
     CDS             complement(8522..8800)
                     /locus_tag="IV334_000891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8942982.1"
                     /translation="MQKKHLFFTLSIAFLSLAHLIFSYFYIRMYGYFNLHGHLNSFMT
                     AAWILRFIIDVYIVICGFFAIREERYKVLPFYLLFFLFNLILPFIFHI"
     gene            9068..11077
                     /gene="metG"
                     /locus_tag="IV334_000892"
     CDS             9068..11077
                     /gene="metG"
                     /locus_tag="IV334_000892"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HAP8942983.1"
                     /translation="MAEKETFYITTPIYYPSGKLHIGNSYTTIACDAIARYKRLMGFD
                     VFYLTGVDEHGQKIETKAEELGVKPQEYVDKMAADVKKLWKTLDISYDKFIRTTDDYH
                     KAAVQKIFDRLLEQGDIYLGEYEGWYSVSDEEFFTETQLAEVYKDDEGNVIGGKAPSG
                     HEVELVKEESYFFRMSKYADRLLEYYEEHPEFIQPESRKNEMINNFIKPGLEDLAVSR
                     TTFTWGVPVKSDPKHVVYVWIDALSNYITALGYGSEDESLFEKYWPADVQMVGKEIVR
                     FHTIYWPIMLMALDLPLPKKVFGHGWLLMKDGKMSKSKGNVVYPEMLVERYGLDALRY
                     YLLRAVPFGSDGVFTPEDFVSRVNYDLANDLGNLLNRTIAMINKYCDGLVPDYASLVT
                     PFDSELSTTAANVVGRYHDAMEKMEFNTALAEIWVLISRANKYIDETEPWVLAKDEEK
                     KAELNSVMIHLAESLRIAAILLQPIMTETPEKIFNQLGLDSETMNLEGLHFGEFPSGT
                     KVVAKGTPIFPRLDMEEEVAFIQEKMSEGTQTNEDTVKWDPEETELVSTKEKQIKFDV
                     FEKVELKVAEVINCQKVEGADKLLQFRLDAGDSQDRQILSGIAEFYPDPSELIGKKVV
                     IVANLKPRKMRGQISQGMILSAEAPDGSLQVIEAPKSMPNGSEIA"
     gene            complement(11556..12143)
                     /locus_tag="IV334_000893"
     CDS             complement(11556..12143)
                     /locus_tag="IV334_000893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8942984.1"
                     /translation="MDFKRSYPKLADYLSNRVDFSRKEQQQSHWHWFTLILFGLMTVA
                     TLSQKLILLTLFIAIVALIKGPVMILWGMLYSFLVGLFPPIGILLSAIFFLLNIGTFT
                     KNWRMTLVGIYFYFYPFGVMALSEVMHWDNHWFIAGSLLLGLILLHVMLTKLYQHYGI
                     GRTIFWYVFSIPFALLTALLPSRLKTKIKGYHKIK"
     gene            12318..13640
                     /locus_tag="IV334_000894"
     CDS             12318..13640
                     /locus_tag="IV334_000894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAP8942985.1"
                     /translation="MKGKLKREINLFGALATVMGTVIGAGVFFKTAAVTASTQSTSLT
                     LLAWLLGGFLTICAGLTVAELATAIPETGGAVKYIEAAYGKLPSFLLGWAQSLIYFPA
                     NIAALSIIFATQLTNLLQLSTDYLLLIAVITAVSVTGLNLLGTKVGASVQSVTLIVKL
                     IPIAVIVIWGLLTPGQGTVQLFPIEAGKDVTFVEGLSSALLATLFAYDGWLGVGAMAG
                     EMKRPEKDLPKAIILGLSFVTVVYLLINFVFLKTLPIDHLAGNLNAASEASDVIFGGI
                     GGKLVTIGILISVYGALNGYTLTGIRVPYAMALEDELPFSKQLTKLSKKFTVPYVPAV
                     FQLAVACIMMSLGSFDFLTDMLIFVMWLFSLLIFIGVFILRKKAPELPRPYKVPLYPI
                     VPIIAILGAIFILGMTMMTQTKLALIGIGVTLIGIPVYYQKKKKRSEE"
     gene            13795..14571
                     /locus_tag="IV334_000895"
     CDS             13795..14571
                     /locus_tag="IV334_000895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HAP8942986.1"
                     /translation="MIFDSHTHLNAEQFNEDIPETIQRAQEMGVTKMAVVGFDTPTIE
                     KSLQLSHDYPNIYSIIGWHPTEAGSYTKEIENKLQEQLTLPKVVALGEIGLDYHWMED
                     PKEVQDKVFRRQIAIAKEMNLPISIHTREALEDTYQILKEEDVRDIGGIMHSFSGDYE
                     WAKRFLDLGMHISFSGVVTFKKALDVQEAAANVPIERLLVETDAPYLAPVPFRGKRNE
                     PGYTRYTVEKIAELRNLPTEEVAAQTWTNAHRLFGLSEHG"
     gene            14564..15133
                     /gene="rnmV"
                     /locus_tag="IV334_000896"
     CDS             14564..15133
                     /gene="rnmV"
                     /locus_tag="IV334_000896"
                     /EC_number="3.1.26.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease M5"
                     /protein_id="HAP8942987.1"
                     /translation="MDKMKIQEIIVVEGKDDTRRLREVVDADTIETIGSAINEEILMQ
                     IEHAQETRGVIVFTDPDFSGEKIRKIIMEAVPQAKHAFLPRTQAKPKKKGSLGVEHAS
                     DAAILEALKKVVTPDISQTAVSEITRQDLLDYGLIAGAQAKEKREKLGDELRIGYTNG
                     KQLEKRLKMFRITPKELAQAMKIVEEQHE"
     gene            15126..16010
                     /gene="rsmA"
                     /locus_tag="IV334_000897"
     CDS             15126..16010
                     /gene="rsmA"
                     /locus_tag="IV334_000897"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HAP8942988.1"
                     /translation="MSEYREIATPSRTKEILLKHGFSFKKSLGQNFLTEPNILRKIVE
                     TAQIDEHTNVIEVGPGIGALTEQLAKHAKQVVAFEIDDRLIPVLADTMQPYDNVTIVH
                     QDILKTDLSTAVRETFHEELPLKVVANLPYYITTPIMMHFLESDLVVDELVVMMQKEV
                     ADRISAEPGTKAYGSLSIAVQYYMEASLAFIVPKTVFVPQPNVDSAILKLTRRDIPAV
                     EVTDEKAFFRLTKAAFQQRRKTLWNNLQHSYGKDDQTKAWLAKSLETAGIDPKRRGET
                     LSLQEFAALSNAMSENMQ"
     gene            16461..17090
                     /locus_tag="IV334_000898"
     CDS             16461..17090
                     /locus_tag="IV334_000898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8942989.1"
                     /translation="MRKNSVRKLTISALLIGMGVIIPMVMPKIMIPPASFTLASHVPL
                     FIAMFFTPGVAVAVALGTTFGFFLTTPVIIALRALSHLVFALIGAWYLQKHPSIVLKN
                     GQFTFANWRFQGFNFVIGVIHSLAEMLVVSIFYFIGNMPETYYSQGYFYTVFILMGIG
                     GLIHSLVDYNIAYFIAGTLSKYFDIPVFTAAKKENRKESIQGYAKKTTY"
     gene            complement(17214..18323)
                     /gene="ugpC"
                     /locus_tag="IV334_000899"
     CDS             complement(17214..18323)
                     /gene="ugpC"
                     /locus_tag="IV334_000899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     ATP-binding protein UgpC"
                     /protein_id="HAP8942990.1"
                     /translation="MVEMALKNVYKKYDNAENYSVTDFNLSIKDREFIVFVGPSGCGK
                     STTLRMIAGLEDISEGELNIGGKVMNDVAPKDRDIAMVFQNYALYPHMTVFDNMAFGL
                     KLRKYDKADIKKRVDNAAEILGLTEYLKRKPAALSGGQRQRVALGRAIVRDAKVFLMD
                     EPLSNLDAKLRVAMRAEIAKLHQRLETTTIYVTHDQTEAMTMADRIVIMKDGFIQQIG
                     TPKEVYDTPVNMFVAGFIGSPAMNFFNVTLKNGVITDGHGLKLRIPEGKNKVLVEKGY
                     EGKEVIFGIRPEDIHSEQVALEASPEAVVKSEVVVSELLGAETMLYTKTGSTEFISKV
                     DARDFHKPGEIVDLAFNISKGHFFDKETEQVIKLP"
     gene            18532..18954
                     /gene="mgsA"
                     /locus_tag="IV334_000900"
     CDS             18532..18954
                     /gene="mgsA"
                     /locus_tag="IV334_000900"
                     /EC_number="4.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010763995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal synthase"
                     /protein_id="HAP8942991.1"
                     /translation="MKIALIAHDRKKPMMVKLATAYKTILEKHELFATGTTGQRITEA
                     TGLPIHRFKSGPLGGDQQIGAMISEDQLDLVIFLRDPLAAQPHEPDVTALIRLSDVYE
                     IPLATNIGTAEVLLRGLEAGFLDFRRIVHEIDKHPLSF"
     gene            19277..19813
                     /locus_tag="IV334_000901"
     CDS             19277..19813
                     /locus_tag="IV334_000901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="folate family ECF transporter S component"
                     /protein_id="HAP8942992.1"
                     /translation="MKKHRLDARMIAIMGLLIALMVTLSRLVAIETPFIKISVTFIPQ
                     VIMGILFGPFWSGIGAVLADLVGMALFSKSAFFIGFTLNAFIEGAIYGFFFYRKEITW
                     KNAILATLSVTLIINLFLTPLWLALMYHVPLFSWVVWAPRLLKTVIWLPIQSIAIYYV
                     GRSIPYKKILRSLAIHAK"
     gene            complement(19853..21616)
                     /locus_tag="IV334_000902"
     CDS             complement(19853..21616)
                     /locus_tag="IV334_000902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP8942993.1"
                     /translation="MKVKKLSLQTLRRFIPYLTAYAKDIWLAVILGIINGIATVVMTL
                     QIGQSIDQMIGEGQVRFNRLFHLLILFGGIVLINVVSQWLIQVLSNRLAYLSVAKLRK
                     DAFAHLNRLPLRYYDQNSHGNIVSRFTNDMDNISIAISAAFNQLFSGVAIIILSFIFM
                     MRLSPLLTLVVLCSTPIIFLVSWIVARASQTNFDRQQRIIGDISGFVTERIGNQKIVK
                     AFQQEEASQQQFELLNADLYEKGQRAQFSSSLTNPSSRFVDHLAYLSIGLVGGLLALK
                     SGSSITVGVISSFTIYSSQFSKPFIELSGITTQLQTAFSGLERAFDLIDQPEEKPDLP
                     DAYALEPKNIKGKVSFDHVSFSYDPKQRLIEDFNFTAYPGQTIAIVGKTGAGKSTLVN
                     LLMRFYDTTKGTITVDDHDIRSIQRDTLRKSFGMVLQDTWLFDSTLRENLRFGNPEAS
                     DAEINDALKASYMYEFVERLPEKLDTKIGGQGLKISDGQRQLLTIARTMISNPPMLIL
                     DEATSSVDTLTEKKIQQAFLAMMEGKTSFVIAHRLSTIKNADQILVMDQGAIVEMGTH
                     DELLQKDGYYRRLYQAQFTAQ"
     gene            complement(21619..23334)
                     /locus_tag="IV334_000903"
     CDS             complement(21619..23334)
                     /locus_tag="IV334_000903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP8942994.1"
                     /translation="MIQLLRYAKDYRKQIILGPFFKFLEAVFELVLPLMMASLIDNGL
                     KMNDRGKIIEMGLWMVAMSVIGLICAIICQYYASVASQGFGTELRNQLIKKINTFSHN
                     ELNHFGTDTLITRMTNDINQLQLALAMVIRLLIRAPFLSIGSVVMAFVIDWEVGLFFL
                     ALLPIFSIILFFIIKKTVPLYQKVQEKLDQLNEHVSQNLSGVRVIRAFAQTKKETEKF
                     DKSTNDLANNYLRVSNISALLSPATTLIMNVGIICLLTVGGIKVNIGSLQQGQVLALI
                     NYMNQMLLALIVVSNLVVIFTRAEASGNRVKEVLDTENSILEAEVSTTPDFSSEAIIA
                     FDHVDFRYTPDSGLSLQNITFQLKRNTVLGIIGPTGSGKTTLTQLIPRFYDVSSGTVI
                     MDGMDVRSWQLDHLRQQIALVPQTSVLFTGTIRENLQWGKKDATEKECWEALKIAQAE
                     EFVRQLPNGLDTKVMENGKNFSGGQKQRLTIARALIAKPALLILDDSLSALDYQTDLD
                     LRKALQTYLGSTVIIISQRVRSIQEAHHILVMDQGKIAAQGTHEELLAQSAEYQEIVA
                     SQEEE"
     gene            complement(24113..24337)
                     /locus_tag="IV334_000904"
     CDS             complement(24113..24337)
                     /locus_tag="IV334_000904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8942995.1"
                     /translation="MKSDKYKNRKHTSWKTFREIIGSYLAVVVFLPLLYMAMTEQTRF
                     IGVDTQYIAETGFLFIFILIFAFYKSFHTK"
     gene            24537..27305
                     /locus_tag="IV334_000905"
     CDS             24537..27305
                     /locus_tag="IV334_000905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAP8942996.1"
                     /translation="MLKKEILCYLENQTAFFDLEHMNEVFTAQRLADHFGVKRNTVSH
                     YLNQLNDEQLLVKINSRPVIYFHKEAFEKQFFKLRTNFYFSIDDLKEEQPFFAQPKDL
                     FSFMIGYDASLKESIEQIKTALYYPDGGLPLLITGESGTGKSYLVNLVYQYCLLHDLL
                     EDSAPFITFNCAQYADNPELLTSNLFGHVKGAYTGAEENKKGAFEAADGGILFLDEVH
                     RLTPEGQEKLFTYLDQGVIYRMGEPNLSRRIKTRLFFATTEEITSHFLTTFIRRIPIR
                     IELPALNQRSRNERLELIYSFFLEEQRKMGSPLSVSGPALSLLTSQKLSGNIGELKNI
                     VKVSAAKAYAEQREQPEIHVTIHHLQKELLAQPITKNTAQQGIYITQDQTLEQLMEKQ
                     QPEQQRIVKSFEQILIDFKKNNCLLYSCEGKLKQEVIQLFDFLLFETSRQEKHELLVY
                     WTQSIRETFRQMETAYQIKFDGNSVYALSYYLYQRSTIKWLPEEHDIIQLIKELEQQV
                     SQSYPSSYHYVQRILELSKIKLDIEDTSMDRILLTIYLKKAKWSKEKRLPKAMIAAHG
                     YATASSMANVVNRLLQEDLFESFDMPLDVTPQQIASEILDYCENSDVSNGLIILVDMG
                     SLKEIHQFFKKQLSVPLLILNNVTTPLAITVGECLQKNVSLEEIAEETVRQIQPEWRL
                     LYPEENKPKALITTCFTGIGTAVHLSELLEKSLPTACQLKIIPYEYQQLKDKKNSDPL
                     FSIYEVVGMIGTTDPDITNIDYLSLEDLISGEKMSVLAEWLDTTMNASEKEIFNQRII
                     RNFSLEKVLDSVTILDTEKVMGEIDHFMRELEVQLNRSISNPKKLALYVHVSCLIERL
                     IRQMPIETYQGLEKWKQCQKEGLSAIKSAFSVIEEHYSVMIPNSEIAYIYDILSEDTE
                     FLSIEEEF"
     gene            27386..27805
                     /locus_tag="IV334_000906"
     CDS             27386..27805
                     /locus_tag="IV334_000906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIA"
                     /protein_id="HAP8942997.1"
                     /translation="MDREIILASHGKFASGILDSLELIYGKNHSITVFDCYTDENFDL
                     AETVRQLFMRYKDKEIIVLTDLFGGSVNNEFLQYINRDHVYLVAGLNLPLLIELVSRL
                     DAEVETTVLIQQVLTDSKKMIQFCNERLNQKIEEDEF"
     gene            27817..28287
                     /locus_tag="IV334_000907"
     CDS             27817..28287
                     /locus_tag="IV334_000907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAP8942998.1"
                     /translation="MIVLTRVDHRLLHGQVAFSWTQTIGADCILIANDDVPTNEIRKT
                     TIKLAKPQGVKLVIKSIDDSIAALKSGVTDKYKLFIVVESIEDAYKLAEAYPEIKTIN
                     LGGIKAKPGTRSIGKAVNILPEEEERLKRLIDKQIEVEIRQVPADKKVDVREVL"
     gene            28304..29083
                     /locus_tag="IV334_000908"
     CDS             28304..29083
                     /locus_tag="IV334_000908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAP8942999.1"
                     /translation="MIVQAVLLGIVAFVAQSEYALGTSLLSRPIVTGLFTGIVLGDVK
                     TGIIMGATLELAFIGSFSVGASIPPDVVTGGILGTAFAITAGAGTETALLLGLPIATL
                     TLILKNIYLGLLIPIMNHKADNYAEEGNYKGVEHMHLLAGIGLSLMLGVVVMVSYMVG
                     SNTIGNLLNMIPDFVQKGLSVATGLIPALGFAMLARLLINKQVAPYFFLGFAIASYLE
                     IPVTGVAIFGAILAVVVVNIMNVRQLPLQTTAGEVEEDEEF"
     gene            29070..29891
                     /locus_tag="IV334_000909"
     CDS             29070..29891
                     /locus_tag="IV334_000909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HAP8943000.1"
                     /translation="MKNSEIKNSKITKKELNHVFWRSFQMEFSWNYERQMNMAYVYAM
                     IPILKKLYQSKEEMAAALKRHLEFFNTTPHIVTLILGITAAMEEENKDDPEFDVTAID
                     SIKTSLMGPLAGLGDSFFWGTLRLIATGVGTSLAMQGNILGPILFILIFNIPHILFRY
                     LATGWGYRLGTGFLKKIQANGMMESLTLGASIIGLMVVGAMTATMITINIPLKIGSGE
                     NAVTVQSIFDDIVPNILCLGAFGIVFYLLKKEVKPLTILIGLAFFGIFGSWIGIF"
     gene            29908..30957
                     /locus_tag="IV334_000910"
     CDS             29908..30957
                     /locus_tag="IV334_000910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HAP8943001.1"
                     /translation="MFTMQDYIYEEKEVLSTILKKNDFSTREHMKKTVNLLILATGSS
                     YNACLAAKPALESYGDLTVDIQEPFYFFNYGKLSPSIDTVIAVSQSGKSASTVEAVKM
                     IQKQGLPVVAITNDVQSPLALEAAQIIDLGAGVESVGFVTKGYSATVLQLLLLGIGIG
                     ISKNKISKKIEQDYMQQLRKIINHLPAIIQKTEEFFDGYQSLFRLAQRFVTIGYGPNW
                     GTAKEAETKLTETIRVPSQGFELEAYMHGPYLEADASHLLFFIEGESVNKERSQKLQR
                     YMSRYVGETLTITTKKARNEKTLGLAIECDEYLSVLALVVPFQLFAYKTAVAKGIDLN
                     KKIFEDFDTVLKSKL"
     gene            30970..31974
                     /locus_tag="IV334_000911"
     CDS             30970..31974
                     /locus_tag="IV334_000911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010760608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HAP8943002.1"
                     /translation="MLKFNEEEQIKAIKGALALRPQVEEIIDKLYAEKFDAVYYLGIG
                     GTYASSMQAVTYMNGKSNLPVFVQHAAEYYTTGNKRLTKDSIVILSSVTGTTQEVVKA
                     VEQIKKVGATLIGFIDKANSKLSQLCDFVVTYPAPGTEQIKFFMAADRLMYLNGEFEA
                     YSEYYEQLERYLPTGLVEAEKKADAFGLSFAEKHRRDSMHYFIGAGNQWGAVYSYAMC
                     YWEEQSWLPSKSIHAAEFLHGTLEIVEETTPVTLFLGEDEQRVLSERVAKLLPKICSN
                     YTLIDTKDYPVEGISEKYRGRVLSFLLMHVVTQRIDAHVEQLNCHPLEIRRYYRQFDY
                     "
     gene            32607..33626
                     /locus_tag="IV334_000912"
     CDS             32607..33626
                     /locus_tag="IV334_000912"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HAP8943003.1"
                     /translation="MKQKIAVLGPGSWGTALAQTLAENGHDVRIWGNVPEQIDEINKY
                     HTNQHFLPDLTIPESIIGYKELAEAVDNADAILFVVPTKAIRSVAQELIGKMNTKPII
                     IHASKGLEQDTHKRISEVLAEEIPEEKRQAIVVLSGPSHAEEVAVHDITTITAASIDQ
                     KAAAYVQKLFMNDYFRIYTNNDVIGVETGAALKNIIAIGAGAIHGLGFGDDAKAAIMT
                     RGLAEISRLGVAMGANPLTFIGLSGVGDLIVTCTSVHSRNWRAGNLLGQGHKLEEVLE
                     NMGMVVEGVATTKAAVELAQQLGVEMPITQTIYNVLYNGEDIKKAAKEIMLRDGKMEN
                     EFSLR"
BASE COUNT        10989 a         5967 c         6882 g         9811 t
ORIGIN      
        1 cacgaaaaaa tcattatttt atgaaaaaga caaaaaaaag tgttgatttt ttaataatta
       61 ttcattaaac tcttaaacaa cttcagaaaa tattaattat tctgaaaatt ccaataaagg
      121 ggtacgtaaa atgaaaaaga aatttacatt tggtttggtt gcggtatgcg gccttgtact
      181 tgctggatgt tatggcggca gttcagccac aagcagtagt tcagcaaaaa gcggttcagc
      241 agatggagga ggcgtattta acttagtcgt gcctcaagaa atgcctacag ctgacttatc
      301 agttgcaaca gatacaatca gttttacagc attgaataac gtttatgaag gaatttaccg
      361 tcttgatgaa gacagcaaac cacagccggc aggagctagc gagttggcag aagtcagtga
      421 agacggatta acttacaaaa tcaaattacg agaagacgca aaatggtcaa atggtgatcc
      481 tgttacagca gcggattacg tttatggctg gcaacgaaca gtagacccag ctacagcttc
      541 tgaatatgct tacctgtatg cgccagttga aaatgcagaa gacattacag ctggaaagaa
      601 agataaatca gaactaggga tcaaagcagt tggtgactat gaattagaaa tcaaattgac
      661 gaaacaaaca ccatatttcc aatatctgct tgctttccca tcatttttcc cacaaaaaca
      721 atccgtagtg gaagaaaatg gggatgccta tgcatctgca agtgataaag ctgtttataa
      781 tggaccattc acattagaag attttgatgg accaggaaca gatacagaat ggacttataa
      841 gaaaaatgat cattactggg acaaagatac agtaaaactt tctgaaatca aagtcagtgt
      901 agtaaaagaa tcttctactg cattgaactt attcaaagat ggccaagcag atgacgtgat
      961 cttgtctggt gagttagcac agcaaaacgc aaacgatcca gcttatactt ctgttaaaga
     1021 agcgcggaca agctatatcg aattcaatca acgtgaaaag aattcaccat tcaacaatgt
     1081 gaacctaaga aaagctattt cttattctat cgatcgtgaa gctttagtaa aacaagtact
     1141 aggtgacgga tcagttgtct ctacaggatt gatcccatca gatatggtaa aaagtccaac
     1201 aacgaacgaa gactttgcaa aagaagctgg aaaattagtt tcttatgaca aagataaagc
     1261 aaaagaatat tgggaaaaag cgaaaaaaga attgggcgtt gattctttag aatttgattt
     1321 gatggcttct gatgatgatt catcgaaaaa agtaatcgaa tatgttcaaa actcaatcca
     1381 agaaaacttg gatggcgtaa cagtcaaacc aacacctgtt ccattctctg tacgtcttga
     1441 ccgctcaact tcgggtgatt ttgacgcagt actaggcgga tgggctgctg actatgcaga
     1501 cccaagcagc tttactgatt tgtttgtcac aggtaattca tacaaccgtg gtcgctggag
     1561 caatgcagaa tatgacgaag cagttaaagc agcttcatca acagacgctg gcgatgaaca
     1621 agcccgttgg gatgatttcc aaaaagcaat caagatcatc tctgatgata tgggtgtcgt
     1681 tccggtgtac caaaaagcag aaggtcacct agtgaatgaa aaaatcaaag gtatcgttca
     1741 ccatgcggca ggcgcttcat gggattacaa atggacatac gttgaagaat aatcgttaat
     1801 accgtgagca tgagaaaaaa cgaggctgcg gcaaatgtcg caacctcgtt ctcatattca
     1861 cttatcctaa caaagaaaaa agcatgaaaa tagcagaaag agcaattagt attccggcaa
     1921 tgatcagcca aaagctgtac ataccgtagc tggcagacga gagcgaggaa aattctggtt
     1981 ttttctttcg gttccttgtt cgtgcaagat ggaccgatcg ctgaaaatga tcgaagaaac
     2041 ccgaagaaaa gacccataaa aaaccgccga taatcagaaa cggcaatgac cataagaata
     2101 gatcattaga taattgaatg agtgtaaagg aatggttgat gagatttttt attaaaataa
     2161 ccagaatcag tagaaaagct actgcgtagg ggatagattt ttttttcatt tgattgctcc
     2221 tttatttttg gttcattatc taataatgta gcgaaacatc tgtcagaagt caaaagctaa
     2281 ggggaaacat tagtgaatag ttatatcaaa tatgtattaa aacgtgtctt tttcatgatc
     2341 atcacgcttt ggttaattgc aacaatcacc tttttcttga tgcaattgct gccgggcacg
     2401 ccatatacga atcaggaacg tttgagtcct gaaacaatcg agatgctaaa taaacaagtc
     2461 ggtttggata aaccagttat cgtacaatat gggatttatc tttctaattt gcttcaagga
     2521 gattttggaa tttccttcca atttaaaaac cagccagtcg cacatttgtt agctggaaga
     2581 atcggaccat ccttgcagtt agggttacag gcaattatct ttggaacagt cctagggacg
     2641 attttaggaa cgatctctgc catgaagcaa aatacatggg cagatacatc ttctacactc
     2701 gtagcgatat taggtcgttc cattccaaac tttgtatttg ctgttttatt acaatatatc
     2761 tttgcaatca aattacaagt tttgccaatc gcaaaatggg atggctttgt gtacacaatc
     2821 ttaccaacaa ttgcgttggc catgtcgccg cttgctgact ctgctcggtt tatccgtacg
     2881 gaaatggtgg aagtactgca tagtgattat gtggaattag caaaagcaaa aggattgagc
     2941 cgttgggaaa tcgcctttaa acatggtctt cgaaatagtt tgattccatt gatgacttta
     3001 ttaggaccgt tagctgtagc attgatgacc ggatcactgg tggtagaaaa tatctttgct
     3061 attcctggta tcggggaaca attcgttaag tcaatcacaa caaatgatta tcctacgatc
     3121 atggctgtaa cgatccttta ctcatttatg ctgatttttg ttattttgat cgtggatcta
     3181 ttgtacggtc tagttgatcc aagaattcgt gtttcagaag ggagccgagg ataaatggaa
     3241 atgataccaa agcgttatga aacagttgca gatatccctg cggatgaatt tcttccatta
     3301 caaaaaaata ccgaagaaga acgtgaacag attactgcac cttctttgaa ttttatccaa
     3361 gattcatggc gccgtttaaa gaaaaacaaa gctgccgtta tttcaatggt tttactattg
     3421 gtcatcattt tgatctctgt gatcacaatc tttgtttctc ctcatgatcc aactgcccaa
     3481 aacgtggctt atatcaattt gcctcctcgt attccgggaa tcaatatcga tggattgaat
     3541 ggaaaagcaa tggttggtgg tgaacttgtt gataaatatg ctcaagcaaa cgtcccttca
     3601 aacgtaaatt tctttttagg aacagacggt ttgggacgtg atgtattaag ccgcttattt
     3661 atgggaatga gaatctcgtt gctgatcgca tttattgctg cattactaga tgtaacgatt
     3721 ggtgtagctt acgggttggt ttctggtttg ttaggcggac gtgtcgataa cgtgatgcaa
     3781 cgttttttgg aaatcttatc aggaattcca aacctcgttg tcatgatttt gatgttggtc
     3841 gtttttgaac caggaatttt ttcaatcgtg gctgctatgg ccatcacaaa ctggatacca
     3901 atggcacgta tcgttcgtgc acaaacaatg aaattaaaag atcaagaata tgttttagct
     3961 ggattgacat taggtgaatc aagaatgaaa attgcattca aacatatttt gccgaatatc
     4021 tctagtgtca ttattattca aatgatgttt agtattccat ctgctatttt cttcgaagcc
     4081 tttttaagct ttatcggttt aggtcttacg cctccgtcag cttcattagg aacgatgttg
     4141 agcgaagggt acaagacatt cctgtatctg ccttatctat tatggattcc ggcagtaact
     4201 ttatcggtta tcatgattgg ctttaactta cttgctgatg gattgcgtga tgcctttgat
     4261 cctaaaatga aagagtgaag acgatgacga aaattttaga agtgaaagac ttagaaatct
     4321 cttttgatac gtatgctgga aaggtacgag cgattcgcgg tgtcaatttt catttgaata
     4381 aaggagaaac attggcgatc gttggggaat ctggaagtgg aaaatcagtg acgaccagaa
     4441 gcatcatgcg cttgctgtca agcaacgcca atatagatgc tggtcaaatc ctctttaaag
     4501 gtaacgatat cgttggaaaa tcagaaagag agatgcaaaa aatccgtggt aaagaaatag
     4561 cgatgatttt ccaagatcca atgacctctt tagatccgac gatgcccatt gggaagcaag
     4621 tcgctgagtc ccttagaaag cataataaag tttcaaaaaa agagggacaa aaagctgcat
     4681 tagaattgct taaattagtt ggaatacccg aagctgagaa acgtatcaac agttatccgc
     4741 atcagttctc aggaggtcag cgtcaacgga tcgtgatcgc gattgcactg gtatgttatc
     4801 cggaaatcct gattgctgat gaacctacaa cagcgcttga tgtgactatt caagcacaga
     4861 ttttggagct gctgaaagaa attcagacaa aaatcgatac atcaattatc tttatcactc
     4921 atgaccttgg tgtagtagcg aatgtcgctg atcgtgtcgc tgtcatgtat ggaggaagaa
     4981 tcgttgaagt tggaacatcg gaagaaattt tctacaaccc gcaacatccc tatacttggg
     5041 gactgcttgg ttcaatgccg acattagata gtgcgaacga tcgattgtat gctattcctg
     5101 gttctccacc ggatttattg aacccgccaa aaggagacgc attctatcca agaaatgaat
     5161 tcgcgatgaa aatcgatgca gaacaagaac caccattttt tgaactatca aaaacacaca
     5221 aggcagcaac ttggctactt gctccgcagg cacccaaagt gacaccgcca gaagaaatcc
     5281 aacgtcgctg ggctatcttt aaagagaaac aaaaacatac ttacggtcaa ggaggaattg
     5341 ctgatgccaa ataaactact tgaagttaaa ggattgaaac agtatttcaa tgtaggaaaa
     5401 aagaatgaag tacatgctgt aaatgacatc agctttcaca tttatgaagg agaaacattc
     5461 ggtttagttg gagaatctgg aagcggaaaa tccactactg gccgtacgat cattcgcttg
     5521 aatgaaccta ctgatggtga gatattattc gacggtcaag atataacgaa gatcaaagat
     5581 aaaaaaggtt tgacgaaatt ccggcatgat gttcagatga tctttcaaga tccatacgct
     5641 tcgttgaatc ctcgtatgaa agtcagagat atcattgccg aaggaattga tgttaatgga
     5701 ctagctaaat catctgaaga gcgtgcaaaa aaagtcgatg atctattgag aactgttgga
     5761 ttgaatccaa gccacggtac tcgttatcct catgaatttt caggtgggca acgccagcgt
     5821 attggtattg cacgagcatt ggcagtaaaa ccgcgtttta tcatttgtga tgaaccaatt
     5881 tctgcactag atgtgtcgat ccaagcccaa gtggtcaatt tgttgcaaga tctccaaaaa
     5941 gaacatcaat tgacctatct atttattgca catgatttgt cgatggttaa acatattagt
     6001 gaccgaatcg gtgtaatgca taacggcttg ttattagaga tgggaacaag tgatgaaatc
     6061 tataatcacg gtgttcatcc ttatacagaa agtttgttat ctgctattcc tctaccagat
     6121 ccagaccatg agagaaaacg cagaagaatc aaatatcaac ctgaaccaga tgacggacaa
     6181 gtacgtcaac ttcgtgaaat cgcaccagaa cattttgtct atgcaactga gcaggaagtc
     6241 ccatattacg caaaaaaact aaaacgacaa aaggaatctc ttctggttgc taactaggag
     6301 ggacatagat gctaaaagct tttaaattcc gtatgtatcc gacagaagaa caaaaacagc
     6361 aattgattcg tacttttgga tgtgctcgat ttacctataa ccatttgctg aaaaagcggc
     6421 agaaaagttg gcaacaaaca ggagtagcca atttttcgct cacacccgct acattaaaaa
     6481 aagaataccc attcttaaaa gaagtagaca gtcttgcttt agcaaatgca cagcttaatt
     6541 tggatcgggc ttttcgcaat tatttcaagg gacgtgccag cttcccaaag cttaaaacga
     6601 aaaaaagtat gtggcaatct tatacgacca acaaccaaac ccgtacgatc tatctggaaa
     6661 atggctactt aaagttacca aaacaaaaag aactaatcaa gataaatcag catcggccag
     6721 ttgaaggatc gattcgctcc gctacgattt ctgctcgata taatgaagaa ttttatgtcg
     6781 cactactttg tgatgtttcg ccagtaaaaa aagaatcttt agctaaatgg atcgggatcg
     6841 cctatcatcc gaagacattg atccaaacat ctcgacctct agaagtaact ttaccaaaat
     6901 ttcaccagac agaagaaaag ctgcaacatg cacagcggaa attgaatgtg aaagtccgct
     6961 ccgctcacca tagaaagatc aggttagatc aggcaagcaa ttatcagaaa caaaagagaa
     7021 aagtaatgga tctttatctg aaacaaaaga accagcgaga agattatctt gaacaattat
     7081 ctgggaaatt ggtcaaacaa tatgactact tatttgtaga atcttttcca aaagaagagg
     7141 cacatgctga tttttctatt cacgactggc ataaattaat aacaaaattg cgttataagt
     7201 cccaatggta taataaaaaa tttcttttga tcaataccga cggagcagaa gaaagcaatt
     7261 ccgtaagaaa gagccaagta gtagaaaaaa tgggcagaca ttcggttatc aagggataaa
     7321 aaatgaatga aactgatagg agcgttaggg ttagccttgt taataaaaag tcttaatgac
     7381 ttttgtttct aggaattttt cttttgaaga aaagctcact aattatgaca aaccaataaa
     7441 acaatagcag aaaaagttcg tacgagcttt ttctgctatt gttaattttt tattcagaaa
     7501 gtttacgaat acatcaaaat tctgaaacaa aaaacgtaac tcacaaagag ttgtttttgc
     7561 gttttttctt gaatgattag gtctgaatct tttgctcaca accttattca tggtgccccg
     7621 gcttgagctg atagattttt gaaggacggc cgattcctac aggacgttca ccgatctcat
     7681 cgaaatattg gagcatagct ttcttaaaat tggaatgatc gatcgtttta taatcgatcc
     7741 ctaagaattt agcaaatact tttcgagctt ccgtaatcgt gaaatcttta cctaaaactt
     7801 gtagaacttg cggctcgtgt tccattttat tgactacacg gttgaatgct ttcaagatga
     7861 tttgactatg gtcaaaagcc aaagtatctt tgccagaaga ctctccagtg actaaatcaa
     7921 gcgagatagc cacttctccg ctagataaat ggatcttgtt actatgacgt tctagtgtga
     7981 accagcgtac ttccttggca tcatctccgg cgattagcgg ttcttctcca ataaaggcta
     8041 agtagctgac tgtgacgacc cagcctcgcg gatctcgatt aggcgtactg aatgtatgca
     8101 gttgctcaat attttctttt gaaataacca caccagtttc ttcctttgtt tctcttaaga
     8161 cgctttctcc agttgactca tccttttgga caaagccacc aggcaatgcc catgaatttc
     8221 gataaggatg cccttttctt tggatcaaca aaactttcag ctgatcttct tctttgttat
     8281 agcacatcag tacgatatcg acagtcaaag agggcttgtc atattctgga cgttcctgtt
     8341 tttgatacca gttaagaaat tcttcttcac ttgctttatg ttcataatat aatttttctt
     8401 cttctttaga agcaaaacgg ttcatctgac cacctccatt gacagtaaaa gtacttttta
     8461 ccttattgta gcagactctg aaaaaaaacc aaggatttac ttgaaaaatc taggttatca
     8521 tttaaatatg aaaaataaat ggaagtatga gattgaataa aaagaaaagc agataaaacg
     8581 gcaatacttt ataccgctct tcacgaatgg caaagaagcc acaaataacg atatatacgt
     8641 caatgataaa acgcaaaatc caagctgctg tcataaaact gttcaaatgc ccatggaggt
     8701 taaaataacc atacatacga atataaaaat agctgaatat taaatgtgct aatgaaagaa
     8761 aagcaatact aagtgtaaaa aaaagatgtt tcttctgcat gattgttcca gtcccttact
     8821 aagatagata gtctttatta taccacgtgt gaggggagaa ataaaaagcc gttttttagt
     8881 agatatgaaa caaaacaaaa acccgcattt attcaaccta ttcatccttt taaaggatag
     8941 attgaacgta gatagaagtc gtttatccag tctttttcat tttgaaaaaa agctaacagc
     9001 atgctttttt tatggtatac tagcaaggaa acgaattcgc atcatgcgat aaaggaggaa
     9061 gagccccgtg gcagaaaagg aaacttttta tatcactacg ccaatctatt atccaagcgg
     9121 caaacttcat atcggcaact cgtatactac gattgcatgt gacgcaattg ctcgttataa
     9181 acggttgatg ggctttgatg tgttttatct cactggagtg gatgaacatg gtcaaaaaat
     9241 cgaaacaaaa gcagaagaac taggtgtaaa accgcaagaa tatgtagata aaatggcggc
     9301 tgatgtcaag aaattatgga aaacgcttga tatcagttat gataaattca ttcgcacgac
     9361 agatgattac cataaagcag ctgttcaaaa aatatttgat cgtttattag aacagggaga
     9421 tatttactta ggtgaatatg aaggctggta ctctgtttct gacgaagaat tctttacaga
     9481 aacacaactt gcagaagtgt acaaagatga cgaaggtaac gtgatcggtg gtaaagcgcc
     9541 aagtggacac gaagtagaat tggtaaaaga agaatcatac ttcttccgca tgagcaaata
     9601 cgcagaccgt ctattggaat actatgaaga acatcccgaa ttcatccagc cagaatctcg
     9661 taaaaacgaa atgatcaaca actttatcaa accaggatta gaggatcttg cagtttcacg
     9721 aacaactttt acttggggag taccagtcaa gagtgatccg aaacatgtgg tgtatgtatg
     9781 gatcgatgca ttatccaatt atattactgc actaggatat ggctccgaag atgaaagtct
     9841 attcgaaaaa tactggcctg cagatgttca gatggtcggt aaagaaatcg ttcgctttca
     9901 tacgatctat tggccaatca tgctgatggc ccttgatttg cctctaccta aaaaagtatt
     9961 tgggcatggc tggctattga tgaaagacgg aaaaatgtct aaatcaaaag gaaatgtcgt
    10021 ttatcctgaa atgcttgtgg aacgttatgg tttagatgca ctccgttatt atttgcttcg
    10081 tgctgttccg tttgggtcag atggtgtctt tacgccagaa gattttgttt cccgtgtcaa
    10141 ttatgatctg gcaaatgatc tagggaactt attgaatcga acgattgcta tgatcaacaa
    10201 atattgtgat ggactcgttc ctgactatgc ctcacttgtc actccatttg atagtgaact
    10261 gtctacgacg gctgctaacg ttgtcggacg ttaccatgat gcaatggaaa aaatggaatt
    10321 caatacagca ttggctgaaa tctgggtgct gatttctcgt gcaaacaaat acattgatga
    10381 aacagaacca tgggtattag caaaagacga agaaaagaaa gctgaattga atagtgtcat
    10441 gatccattta gctgaatctt tgcgtatcgc agcgatatta cttcagccaa tcatgacaga
    10501 aacgccagag aagatcttca atcaactagg attggactct gaaacaatga atcttgaagg
    10561 attacacttt ggcgaatttc cttcaggaac aaaagtagtt gcaaaaggta caccaatctt
    10621 cccacgttta gatatggaag aagaagttgc ttttattcaa gaaaaaatgt ctgaaggtac
    10681 tcaaacaaac gaagatacag tcaaatggga tccagaagaa acagaacttg tttcaacgaa
    10741 agaaaaacaa atcaaatttg atgtatttga aaaagtagaa ctaaaagtag cagaagtcat
    10801 caactgtcaa aaagtagaag gggcagataa gttgctgcaa ttccgactag atgctggaga
    10861 cagccaagac cgtcaaatct tgtcaggaat cgctgaattt tatcctgatc caagcgaatt
    10921 gatcgggaaa aaagtcgtca ttgtagcaaa tctgaaacca cgaaagatgc gtggacaaat
    10981 cagtcaagga atgatccttt ctgcagaagc accagatggc tctttacaag taatcgaagc
    11041 accaaaatcc atgccaaatg gctcagaaat cgcatagaaa ttcaaagggg gtatgacact
    11101 agttgtgtca tacccccttt ttccgaaatt tgagaagcaa ttttcgtaaa ttccttctta
    11161 ttacttgaag ccaatcatat aatacagaaa cagagaacta tccaaaataa atcctagaaa
    11221 aagaagtacg agcttcgctg atttttgtta atttagaata atactaatag gaatcgtaaa
    11281 aacagaaaaa aagcataatg ataaagggtc gggcagattt taattgattc atctggtact
    11341 cttttttatt gtccttacat tcataaagtt tgaatgcaat gactttatta catctatatt
    11401 tatatctaac agtagcgttg atgtgttaat tataagttct atcactttat gttcaattag
    11461 tttgtgctct tatatgtgtg attgaaaata tagataaaaa aacaatcatc ggctttgact
    11521 tcgatgattg ttttctttat tttgtaaaaa tcctatcact tgattttatg gtaacctttt
    11581 atttttgtct tcaaacggct tggtaagaga gcagtaagca gtgcaaatgg gatcgaaaag
    11641 acataccaga aaatcgtacg tccgatacca taatgttgat agagctttgt caacatcacg
    11701 tgtaacaaga tcaaccctaa aagcaaggag ccagctataa accaatgatt atcccaatgc
    11761 atgacttcac taagtgccat cactccaaaa ggatagaaat aaaaatagat cccgactaag
    11821 gtcatccgcc agttctttgt aaatgttccg atattcaata aaaagaaaat cgctgaaagc
    11881 aaaataccga taggcggaaa aagcccaacc aaaaaggaat acaacatccc ccacaagatc
    11941 atcacaggac ctttgatcaa tgcaacgatt gcgatgaaca aagtcagcaa tattagtttt
    12001 tgtgataacg ttgcaacagt cattaatcca aataaaatta atgtgaacca atgccaatgg
    12061 gactgctgtt gttctttacg ggaaaaatcg acccggttgg ataaataatc tgctaatttc
    12121 ggataagaac gcttaaaatc cataaactgt tcctccttcg tggaaaaggt aggtttagta
    12181 tacgaaaaat aaaaaagaaa tacatccgtc ttaagaggga gtttttcact gaaagtggag
    12241 aaaaaagaat ggtaaattaa aatattctca gttatactga aaattatacc aaatttcaaa
    12301 gaagaaagag gatgggaatg aaaggcaaac taaaacgaga aatcaatctg tttggcgcat
    12361 tagctaccgt tatgggaacc gtgatcggtg caggcgtctt cttcaaaact gctgctgtta
    12421 ctgctagtac tcagtctaca agtttgacat tgctagcttg gcttttgggt ggtttcttaa
    12481 cgatctgtgc tggattgaca gtggctgaat tagctacagc aatcccggaa actggtggag
    12541 ctgtaaaata tattgaagct gcgtatggaa aattgcccag ctttttatta ggatgggcgc
    12601 aaagcttgat ttactttcct gctaatattg ctgcattatc gattatcttt gcgacacaat
    12661 tgacgaactt attacaatta tctaccgatt atcttttgtt gattgcagtg atcaccgctg
    12721 tttctgtgac agggttgaat ttgttaggga caaaagtcgg tgcttctgta caatcagtga
    12781 cgttgattgt taaattaata ccaattgctg tgattgtcat atggggatta ttgaccccag
    12841 gacaaggaac tgtccagtta tttccaatcg aagctggaaa agatgttact tttgtcgaag
    12901 gattgagcag tgcgttgtta gcaacgttgt ttgcttatga cggctggtta ggtgtcggtg
    12961 caatggcagg ggaaatgaaa cggccggaaa aagatttgcc taaagcaatt attcttggtt
    13021 tgagttttgt aacagtcgtt tatctattga tcaattttgt ttttttgaaa actttgccga
    13081 ttgatcatct tgccggtaat ttgaatgcag cttctgaagc atcagacgtc atttttggag
    13141 gaatcggtgg aaagctggtc actattggga tcttgatctc ggtatacggt gcattgaacg
    13201 gttatacact aactgggatc cgcgtgcctt atgcgatggc tttagaagac gagctacctt
    13261 tcagtaaaca gctgacaaag ctttcaaaga agtttacggt tccttatgtt cctgcggttt
    13321 tccagttagc agtagcttgt attatgatga gccttggttc tttcgacttt ttaacagaca
    13381 tgttgatttt tgtgatgtgg ctattcagtt tactgatttt tatcggtgtc tttattttaa
    13441 gaaaaaaagc gccggaattg ccacgtccat ataaagtacc actttatcca atcgttccga
    13501 ttattgcgat tctcggtgct attttcattt taggtatgac gatgatgacg caaacgaaat
    13561 tagcattgat cggtataggt gttaccttga taggtatccc agtctattat caaaaaaaga
    13621 aaaaacgctc agaagagtaa aatatttcaa caacagacag aaaaagtacg aactaggtcc
    13681 tctagatctt cgtgcttttt ctatttcaat aaaacaagtt cttctatttt gatttttgct
    13741 acttctgcaa gtagtgctta tgtgctaaag taagaatgaa aaggagagat aaacatgata
    13801 tttgattctc atacccattt aaatgctgaa cagttcaatg aagatattcc agaaacgatt
    13861 cagcgagcac aagaaatggg ggtaacgaaa atggcggtcg tgggcttcga tacgccaacg
    13921 attgaaaagt ctttgcaatt aagccatgac tatccaaata tatacagtat tatcggctgg
    13981 catccgacag aggctggcag ttatacgaaa gaaatcgaaa ataaattaca agaacaattg
    14041 actttaccaa aggttgtagc attaggggaa attggcttgg attatcattg gatggaagac
    14101 ccgaaagaag tccaagataa agtatttcgc agacagattg ctatcgcaaa agaaatgaac
    14161 ctaccaatca gtattcatac tagagaagca cttgaagaca cgtatcaaat cctaaaagaa
    14221 gaagatgttc gagatatcgg tggaatcatg catagtttca gtggtgatta tgaatgggca
    14281 aaacgatttc ttgacttagg gatgcatatt tcatttagcg gtgtcgtcac tttcaaaaaa
    14341 gcattagacg tccaagaggc tgcagcaaat gtaccaatcg aacgattact tgtggaaaca
    14401 gatgcccctt atctggcacc agttcctttt agaggaaagc gaaatgaacc aggctatact
    14461 cgttatacag tagaaaagat tgctgaactt cggaatcttc cgacagaaga agtggctgcc
    14521 caaacatgga caaatgcgca ccgattattt ggtctaagcg aacatggata agatgaagat
    14581 ccaagagatt atagtcgttg aaggaaaaga cgatacaaga aggctgcggg aagtagttga
    14641 tgcagatacg atagaaacaa tagggtctgc aatcaatgaa gagatcctga tgcaaattga
    14701 acatgcccaa gagactcgtg gagtgatcgt ttttaccgat cctgattttt caggagaaaa
    14761 aataagaaaa atcattatgg aagctgttcc tcaggcaaaa catgcttttt tgccgcgcac
    14821 acaagctaaa ccaaagaaaa agggaagcct tggtgtcgag catgcaagtg atgctgctat
    14881 tttagaagca ttgaaaaagg tcgtgacgcc agacatttcc caaacggctg tttctgaaat
    14941 cactagacaa gaccttttag actatggttt aatagctggg gcacaagcaa aagaaaaacg
    15001 agaaaaacta ggtgacgagt tgcgcatcgg ttacacgaac ggcaaacaat tggaaaaacg
    15061 tttgaagatg tttcgtatta cgccgaaaga actcgcccaa gcaatgaaaa tagtggagga
    15121 acaacatgag tgaatatcgt gaaattgcga caccctctag aacaaaagag attttattga
    15181 aacatggttt ttcatttaaa aaaagtttag gacaaaattt tctgactgaa ccaaatattt
    15241 taaggaaaat cgtagaaact gcgcaaatag atgaacatac gaacgtgatc gaagttggac
    15301 caggaatcgg tgcattgacg gaacaattag ccaagcatgc aaaacaagtc gtagcatttg
    15361 agatcgatga tcgtttgatc cctgtattgg ctgatacgat gcaaccctat gacaatgtga
    15421 ctattgtaca tcaagatatt ttaaagaccg atttgtcaac agctgttaga gaaacatttc
    15481 atgaggagct tccgttaaaa gtcgtagcga atttacctta ctatattacg acaccgatca
    15541 tgatgcattt tcttgaatca gatttggttg tggatgaact agtcgtcatg atgcaaaaag
    15601 aagtagctga tcgtatctct gctgaaccag gaacgaaagc ttatggttca ttatcgattg
    15661 ctgttcaata ttatatggaa gccagtttag cattcatcgt accaaaaaca gtatttgtcc
    15721 ctcagccaaa tgtagattca gcgattttga aactgacaag aagagatata ccggcagtcg
    15781 aagtgacaga cgaaaaagca tttttccgat tgacaaaagc ggcatttcag caaagaagaa
    15841 aaacgctttg gaataacttg cagcattcgt atggcaaaga tgaccagaca aaagcatggt
    15901 tagcaaaaag cttggaaaca gctggaatcg atccaaaacg tcgtggggag actttgtcat
    15961 tacaagaatt cgccgcatta agcaatgcga tgtcagaaaa tatgcagtaa aaaaagaggt
    16021 tgtgacagaa gttgtgaaaa agctgtcctg catcaagtaa taagaacagc caaacaaaaa
    16081 tagttcctca tgtttttcgt ttgtttgggc ttgttaccgc agatgcaagc ttttgaacac
    16141 cgtttaagaa agaggttgtg ggcctgccgt ttaactctaa gtcacaagga acaatttcaa
    16201 aaaacagctt ctcatgtttt tgagttagtt ggtcttatta cttgatgcaa gcttttgacc
    16261 actattatgc cgaacaagga gatgtgacac aaattttgtc acatctccta tttttgtttc
    16321 ttgatcaaaa aattcttata cttgatactc acactcattt tgtatggatt tagaaaagaa
    16381 tatgtgtctt gacagttatt agtaagtgag ttatactaag tcgacattta tgtgtcaaga
    16441 catattagga gagagaaaaa atgagaaaaa attcagttcg aaaactaacg atttctgctt
    16501 tattgattgg gatgggggtc attatcccaa tggtcatgcc taaaatcatg attccaccag
    16561 cttcctttac tttggcgtct catgtgccat tatttattgc gatgtttttt acaccaggag
    16621 ttgcagtggc tgttgcgcta ggtacgacat ttggtttctt tttaacaacg ccggttatca
    16681 tcgcattgcg agcattgtca catcttgtgt ttgcgttgat tggggcttgg tacttgcaaa
    16741 agcatccgag tatcgtttta aaaaatggac agtttacttt tgctaattgg cgtttccaag
    16801 ggttcaattt tgtgatcggt gtgattcatt ctttagcaga aatgctagta gtcagcatct
    16861 tttactttat tggtaatatg cctgaaacat attattctca aggatatttt tatacagtat
    16921 tcatcttgat ggggattggt gggcttatcc acagtttagt tgattataat attgcttatt
    16981 ttatagcagg aacactcagc aaatattttg atatccccgt atttacggca gcaaaaaaag
    17041 aaaatcggaa agagtcgatt caagggtacg cgaagaaaac gacatattag acatgatctc
    17101 tcaaaaaaaa aactctcatc tagtaatgga taataaatcc tctaagatga gagttttttg
    17161 tcctaaaaag agctgcacca acatcttctg gcacagctct aacagggggc aatttatgga
    17221 agtttgatga cttgttctgt ttctttatca aagaagtggc ctttgctgat gttgaaggca
    17281 aggtcgacta tttcgcctgg tttatggaaa tctcttgcat ctacttttga gatgaactca
    17341 gtagaaccag tttttgtgta aagcatcgtt tcagctccaa gtaattcgga aacgacaact
    17401 tctgatttca ctacggcttc aggtgaagct tcaagggcta cttgttcact gtggatgtct
    17461 tctggacgaa taccgaagat aacttctttt ccttcataac ctttttcaac taatactttg
    17521 ttttttcctt caggaatacg aagcttaagc ccatgtccat ctgtgataac accatttttc
    17581 aatgtcacat taaagaagtt catggcagga gatccaataa agccggcaac gaacatattg
    17641 acgggtgtat cataaacttc tttcggtgta ccgatttgtt ggataaatcc gtctttcatg
    17701 ataacgattc ggtcagccat agtcattgct tctgtctgat cgtgagtaac atagatggtc
    17761 gttgtttcaa gacgctggtg aagtttggca atctcagcac gcatggctac acgtaattta
    17821 gcatctaaat ttgataatgg ctcatccatt aagaagactt ttgcatcccg aacgattgcc
    17881 cgtcccaatg ccacacgttg gcgttgtcca ccagataaag cagctggttt tcgtttcaag
    17941 tattctgtca gacctaagat ctctgctgca ttgtccacac gttttttgat gtctgcttta
    18001 tcatatttac gtaatttcaa gccaaaagcc atattatcaa aaacagtcat atgaggatac
    18061 aatgcgtagt tttggaaaac catggcaata tcacggtctt taggtgccac gtcattcatg
    18121 acttttccgc cgatatttag ttctccttca gagatatctt ccagtcccgc gatcattcgc
    18181 aaagtagttg actttccaca accggaagga ccaacaaaaa cgataaattc acggtctttg
    18241 attgatagat taaaatcagt tacagaataa ttttctgcat tgtcatattt tttatatacg
    18301 tttttcagag ccatttctac cataatacgt tccacacttt cttttttagt tttattaact
    18361 atttaaagta taaatgaaag cgttaactaa cttcaatgtc atgttgaaca agaatttttt
    18421 cttttttttg tcaatgtgct agttttaagc tttaataaat atccataaga aataaaaact
    18481 gatacactgg agtaaataag tatgttaaca atatcaaaag gagtgtctct tatgaaaatc
    18541 gctttgatcg ctcatgaccg aaaaaaacct atgatggtga aattagcaac agcgtataaa
    18601 acaatattgg aaaaacatga actttttgct accgggacga ctgggcagag gatcacggaa
    18661 gcaaccggct tgccgatcca tcggtttaaa tccggaccgt taggcgggga tcagcaaatt
    18721 ggagccatga tttcggagga tcagttagat ttggtgattt ttcttcgaga cccgctggct
    18781 gcacagcccc atgaaccaga tgtaacagca cttatccgtt tatctgatgt ctatgagatt
    18841 ccgttagcta cgaatatcgg aacagctgag gtgctactaa gaggactgga agccggcttc
    18901 ctagattttc gaagaatcgt ccatgaaata gacaagcatc cactttcatt ttgatggata
    18961 agcgtatgga aaaataacgt ttaatgttcg cctatatgta gctataaaag ctaaacaaac
    19021 tgataatatg cgatataata cgaacgtatt ggataatata aacaataata ttagtttttc
    19081 gtatttgcag taaatgtgaa aatatgctat agtttctgtg ggaaaagatc ccaaaaataa
    19141 tacagagtag aaaataaagc gttaagtgct aagaggatgg gatgttgcct cttggacgaa
    19201 ggacgtaatg ttcgcggttt tattttcgca ttcgctgcat gatacgatgt agcagctctt
    19261 aaaggagatg ctaaaaatga agaaacaccg gttagacgca cgtatgattg ctattatggg
    19321 cttattgatt gcattgatgg tcactttgtc ccgactggta gcaatcgaga ccccatttat
    19381 taaaatcagt gtcacattta ttccacaagt gatcatgggc attctttttg gtcctttttg
    19441 gtcagggata ggcgcagttt tagctgattt agtaggaatg gccctcttct cgaaatctgc
    19501 attctttatt ggatttacat tgaatgcatt tattgaagga gcaatctacg gattcttttt
    19561 ctatcgaaaa gagatcactt ggaaaaatgc cattctggct acgcttagtg tcacgctgat
    19621 catcaactta tttttgacac cattatggct tgcgttgatg taccatgtgc cattgttcag
    19681 ctgggtcgtt tgggcgcccc gcctgttaaa aacagtaata tggttaccga ttcaatcaat
    19741 tgccatttat tacgtaggac gtagtattcc ttacaaaaaa attttaagaa gtttagcgat
    19801 ccacgctaaa taaaaaacaa gcagtcttcc catcccagaa gactgcttgt ttttattgtg
    19861 ctgtgaattg tgcttgatag agccgtcggt aataaccatc tttttgaagc agctcatcat
    19921 gggtgcccat ttcgacaata gctccctgat ccatcactaa tatctggtcc gcatttttga
    19981 ttgtggacag gcgatgggca atcacaaaac tggtttttcc ttccatcatt gctaagaagg
    20041 cttgttggat ttttttctct gtcaatgtat cgactgagct ggtagcttca tctaagatca
    20101 acataggggg attactgatc atcgttcgag caatcgtcaa cagttggcgt tgtccatctg
    20161 atatctttaa cccttgaccg ccaatcttag tatctaattt ttccggcaaa cgttctacaa
    20221 attcatacat ataagaggct tttagggcat cattgatttc tgcgtcactt gcttccggat
    20281 tcccaaaacg aagattttct cgcaatgtac tgtcaaatag ccaagtatct tgtaacacca
    20341 ttccaaaact tttacgtaat gtatcacgtt ggatagagcg gatgtcatgg tcatcgactg
    20401 taattgttcc ttttgttgtg tcataaaaac gcatcagcaa attaacaagt gtggattttc
    20461 cggcccctgt ttttcctact atagcaatgg tttgacctgg ataagcagta aaattgaaat
    20521 cttcgatcaa acgctgcttg gggtcataag aaaaagaaac atgatcaaaa gagacttttc
    20581 ctttgatatt tttgggttcc aaggcatagg catctggcaa atcaggcttt tcttctggct
    20641 gatcgatcaa atcaaatgct cgttctagac cagaaaaagc ggtttgcagt tgtgtcgtga
    20701 ttccagaaag ttcgataaat ggtttagaaa actgacttga gtagatcgta aagctggaaa
    20761 tcacaccaac agtgattgac gaaccactct tcaatgccaa taggccgcca actaaaccaa
    20821 tcgataaata agcaaggtga tctacaaaac gagaagacgg gtttgtcaag gaagaagaaa
    20881 attgtgcccg ttgtcctttt tcatatagat cagcatttag cagttcaaat tgctgttgac
    20941 tagcttcttc ttgctgaaaa gctttcacga ttttctggtt accgattcgt tcggtcacaa
    21001 agcccgagat atccccaata atcctttgct gtctatcgaa atttgtttgt gaagcacgag
    21061 caacgatcca actcacaagg aaaataattg gtgtagaaca aagaacgacc aatgtcagaa
    21121 gtggacttag acgcatcata aaaataaacg aaagaatgat gattgccact ccagaaaata
    21181 attgattgaa ggccgcagaa attgcaatgg aaatattgtc catatcattt gtaaatcgac
    21241 tgacgatatt tccgtgggag ttttgatcat aataacgaag tggcaatcga ttcagatgag
    21301 caaatgcatc ttttcttaat tttgcaacgg ataagtaagc taaccgattg cttaacactt
    21361 ggatgagcca ttgactgact acgttgatta aaacgatacc accgaataga ataagtaaat
    21421 ggaacagtcg attgaagcgg acctgtcctt ctccaatcat ttgatcgata ctttgaccaa
    21481 tttgtaacgt catcacaact gttgcaatcc cattgatgat tcctagtatc acagctagcc
    21541 aaatgtcttt tgcataggca gtaaggtaag gaatgaatcg gcggagcgtc tgcaaggata
    21601 atttttttac tttcataact attcttcctc ctgtgatgcg acgatttcct gatattctgc
    21661 tgattgagca agtaattctt catgggtacc ctgagcagca atcttacctt gatccatcac
    21721 taagatatga tgtgcttctt gaatagagcg gacacgttgg gagataataa tcactgtact
    21781 gccaagataa gtctgtagcg ccttacgcaa atccaagtct gtttgataat ccaacgcact
    21841 taaagagtca tctaaaatca gtaatgctgg cttggcaatc aaagctcttg caatcgtcag
    21901 tcgctgtttt tgtccgccag agaaattttt cccattttcc atgacttttg tatctaaacc
    21961 atttggaagc tgacggacaa attcttctgc ctgagcgatt ttcagagctt cccaacattc
    22021 tttttctgtg gcgtctttct taccccattg aaggttttca cgaatagtcc cggtaaatag
    22081 tacagaagtt tggggaacaa gagcaatttg ctgacggaga tgatccagct gccaagaacg
    22141 aacatccatt ccgtccatta taactgtacc ggaactgaca tcatagaaac gcggaatcaa
    22201 ttgtgtcaaa gtcgttttcc cgcttccggt tggaccaatg attccaagga ctgtatttcg
    22261 cttcagctga aatgtaatat tttggagaga aagaccagaa tcaggagtat aacggaagtc
    22321 aacatggtca aaagcgataa ttgcttcaga agaaaaatcg ggagtagtac tgacctcagc
    22381 ttcaagaatc gaattttctg tgtctagcac ctctttcacc cgatttcctg aagcttcggc
    22441 acgtgtgaag atcacgacga ggtttgaaac aacgatcagt gccaaaagca tttgattcat
    22501 ataattgatc aaagcaagaa cttgtccttg ttgcaagcta ccaatattta ctttgatccc
    22561 gccaactgtc agcaaacaga tgatcccgac attcatgatc aaggttgtag caggtgataa
    22621 tagggcagag atattggaaa cacgtaaata attgttcgct agatcatttg tgcttttatc
    22681 gaacttctct gtttcttttt ttgtctgcgc aaatgcgcgg atgacacgaa caccgctaag
    22741 attctgacta acgtgttcat tgagctgatc tagtttttct tgcacttttt ggtagagagg
    22801 aacggttttt ttgatgataa aaaataaaat gattgagaaa ataggcaata atgctaagaa
    22861 aaacagacct acttcccagt cgatcacaaa agccatgacc actgacccga tacttaaaaa
    22921 cggtgctctg atcaataaac gaatgaccat tgctaaagct agctgaagct gattgatgtc
    22981 gtttgtcatt cgggtgatca aggtatctgt tccaaaatga ttcaactcgt tgtgagagaa
    23041 agtattgatt tttttgatta gctgattacg taactctgta ccaaatcctt gtgaagctac
    23101 tgaagcatag tattggcaaa tgatagcaca aatcagacca atcacagaca tagcgaccat
    23161 ccataagccc atttcaatga tttttcctcg gtcattcatt tttagtccat tatcgatcaa
    23221 ggaagccatc attaaaggca ggacaagttc gaagacagct tcaagaaatt tgaagaacgg
    23281 gcccaaaatg atttgcttac gatagtcttt tgcataacgt aataactgaa tcatatgatt
    23341 cctcctatta tttatttgtc cacaattgaa aaaacaagta ctaccacatt gtagcttagg
    23401 aaatttagaa tgaaaagaga acaagccttt tcataaatga aagttttatt atgaagaagc
    23461 tttagaaatt gaatagacta ttttttttaa aaaaaagaaa atgagaaaac atgccagtct
    23521 cttcattcat aggaacgtct attttactta atttttctat aaatgaattc tgcataaaaa
    23581 atatatcaag taaatgttta tagatgaaac tctatttact tcttttttaa tttgttcttg
    23641 caatcttttt tgctacgtaa tttttgtaaa aaagtgggtt gagagaaatc taattgattt
    23701 tcaaagaagg agtatgaaaa tgagaaaaat atttattcgt ttattgtggg tactatctta
    23761 gaggaataaa taatagtgga gaaaactatg gtttggcgca gattctatca ggtttgatgg
    23821 aacgaagaca acctcccgac atcaagtcaa aagctacttg agattttgat ctgaaaataa
    23881 aaaaagaaaa caaggtagga acatcaatgt ttctaccttg ttttcgtaat caatgggaaa
    23941 aaactaagag tggtgtacgt aatcaataaa aaggatggtg acaacagtac ctctttactc
    24001 tctgaaagca tcataactcg tttttcctca ttatgccaat aaattgttgt tataccacat
    24061 ataaggattt gtaatagcta aaaacagaca ggaaagttga aatctgtgaa tcttattttg
    24121 tatggaagga tttatagaac gcaaaaatca agataaagat gaataaaaat cctgtttcgg
    24181 ctatatattg cgtatctacg ccaataaaac gagtttgttc tgtcatagcc atataaagca
    24241 aaggcaaaaa tacaactaca gcgagataag atccgatgat ttctcgaaaa gttttccagg
    24301 aagtatgttt cctgttttta tatttatccg atttcattga atagtcatag cctccttatt
    24361 ctttcatatg ttgaaattcc agtataaaaa ctggaaggaa ctgtacgttt tttagaataa
    24421 aaattataag ggataagtag gctgctgtct ataataagat atatttttca attctttatt
    24481 aaagatttta ttgacaaaat gaatccgttt tctatgatgg aggaggggtg aataagttgt
    24541 tgaagaagga aattttatgt tacttagaga accagacagc ctttttcgat ttagaacata
    24601 tgaacgaggt ctttacagca caaaggttag ctgatcattt tggggtaaaa agaaacacag
    24661 tcagtcatta tttgaaccag ttaaatgatg agcaactact agtcaaaatc aattctcgac
    24721 cagtgattta tttccacaaa gaagcttttg aaaaacaatt ttttaagtta cgcacgaatt
    24781 tttatttttc tatcgatgat ctcaaagagg aacagccttt tttcgcgcaa ccaaaagact
    24841 tattctcttt tatgatcgga tacgacgcca gtttgaaaga aagtatcgaa caaatcaaaa
    24901 cagctcttta ctatccagat ggcggattac cacttttgat tacaggcgaa agtggcacag
    24961 gtaaaagtta tttagttaat ttggtctatc agtattgtct gcttcatgat ctgctagaag
    25021 attctgcacc attcatcact tttaattgtg cacaatatgc agataatcct gaacttttga
    25081 ctagcaattt gtttggacat gttaagggag cgtacactgg tgcagaagaa aataaaaaag
    25141 gggcttttga agcagcagat ggtgggatct tgtttttaga cgaggtacat cgcttaacac
    25201 cagaaggtca ggaaaaactg ttcacctatc ttgatcaagg tgtgatttat cgtatgggag
    25261 aacctaattt gtcacgccgg atcaaaacac gtctcttttt tgctacgact gaagagatta
    25321 ctagtcattt cttaacaact ttcattcgaa gaatacctat ccgaatcgaa ctacctgcac
    25381 ttaatcagcg tagtcgtaat gaacggctag aactgattta ctcttttttt ctcgaagaac
    25441 aaagaaaaat ggggagccct ttgtcagtca gtggaccagc tttgtcactt ttaacaagtc
    25501 agaaattatc cggaaacata ggagagctga aaaatatcgt caaagtttca gcagcaaaag
    25561 catatgctga acagcgtgaa cagcctgaaa tccatgtcac gatccatcat ttgcaaaaag
    25621 aactacttgc gcagcctatt actaaaaata cagcacagca aggaatctat attacgcaag
    25681 accagacctt agagcaatta atggagaagc aacagcccga acagcagcga atcgttaaaa
    25741 gttttgagca aatcctaatt gatttcaaga aaaataactg tctgctatat tcttgcgaag
    25801 gaaaattaaa gcaagaagtg attcaattat ttgatttttt gctgtttgag accagtagac
    25861 aagaaaaaca cgagctgctg gtttattgga cacaatctat ccgagagacc tttcgacaaa
    25921 tggaaactgc ctatcaaatc aaattcgatg gaaacagtgt ctatgccttg agttactatc
    25981 tctatcagcg aagcactatc aaatggttac cggaagaaca tgatattatc caactaatca
    26041 aagaattaga acaacaagtt tctcagtctt atccatctag ttatcactat gtccaacgaa
    26101 ttctggaatt aagtaagatc aaattagata ttgaagacac aagtatggat cggattttac
    26161 tgacgatcta tctaaaaaaa gcgaaatggt caaaagaaaa acgactgcca aaagcgatga
    26221 ttgctgctca cggttatgca actgctagca gtatggcaaa tgtagtaaat cgtttattac
    26281 aagaggatct atttgaatca tttgatatgc cactagatgt cacaccacag cagattgctt
    26341 cagaaatcct tgattattgc gaaaacagcg atgtttcaaa tggattgatc atattagtag
    26401 acatgggatc tctgaaagaa atccatcagt tttttaaaaa acagttatcc gtaccgcttt
    26461 tgattttgaa taatgtgacg acacctttag caatcacagt aggggaatgc ctgcaaaaaa
    26521 atgtatcatt agaagagata gcagaagaga ctgttcgaca gatccagcca gaatggcgac
    26581 tgctctatcc agaggaaaac aaacccaaag cactcattac tacttgcttc actggaatcg
    26641 gtacggcggt ccacttaagt gaactgctag aaaaaagctt acctacggct tgtcagttga
    26701 aaatcatccc atatgaatat cagcaattaa aagataagaa aaacagtgac ccattatttt
    26761 ccatatacga agtcgtagga atgattggca ccacagatcc tgatatcacc aatatcgact
    26821 atctttcatt agaagacctc atctcggggg aaaagatgtc tgtactagct gaatggttgg
    26881 acactactat gaatgcttca gagaaggaaa tattcaatca gcgcatcatt cgtaattttt
    26941 cattggaaaa agtactggat tccgtgacta ttctagatac agagaaggtg atgggagaaa
    27001 tcgatcattt tatgagagaa ttagaggtgc agttgaatcg aagcatcagc aatcccaaaa
    27061 aattagcgtt gtatgtccat gtgagctgtc tcattgaacg attgatccga caaatgccaa
    27121 tcgaaaccta tcaaggatta gagaaatgga agcagtgtca aaaagaaggg ttatctgcta
    27181 ttaaatctgc gtttagtgtc attgaagagc attatagtgt catgatcccc aattctgaga
    27241 ttgcctatat ctatgatatt ttgagtgaag atacagagtt tctctcgata gaagaagaat
    27301 tttgaacata agcaagttgt tgccactaat tttggcatgc aacttgcttt tttagtatgt
    27361 gtaagtgaaa agaggagatg aaaaagtgga tagagaaatt atcttagctt cccatggaaa
    27421 atttgcttca gggatattag attcattaga gctgatctat gggaaaaatc attctattac
    27481 tgtattcgat tgctatactg atgagaattt tgatttagcg gaaacagtaa ggcagctttt
    27541 tatgaggtac aaagataaag aaatcattgt tctgacggat ctttttggcg gaagtgtcaa
    27601 caacgaattc ttacaatata tcaatcggga tcatgtctat ctcgttgccg gattgaattt
    27661 gccactattg attgagctag tatctcggtt ggacgctgaa gtagagacaa ctgttttgat
    27721 ccagcaggtg ctaactgatt caaaaaaaat gatccagttt tgtaacgagc ggctcaatca
    27781 gaaaatagaa gaagacgaat tttaggagga agaaaaatga ttgtattgac aagagtcgac
    27841 catcggttat tacacggaca agtagctttt tcatggacac aaacaatagg cgcagactgt
    27901 attttgattg ctaatgatga tgtacctacg aatgagatac gaaagacaac gatcaaatta
    27961 gccaagccac aaggagtcaa actagtgatc aaatcaattg atgactcgat tgccgcgttg
    28021 aaaagcgggg tgacagataa atataagtta ttcatcgtag tggaatcgat cgaggatgca
    28081 tataagctgg cagaagcgta tccagaaatc aaaaccatca acttaggagg gatcaaggca
    28141 aaacctggta ctcgcagcat cggaaaagcg gtaaatattt taccagaaga ggaggaacgc
    28201 ttgaaacgat taattgacaa acaaatagaa gtagaaatcc gtcaagtacc agcagataaa
    28261 aaagtggatg taagagaagt tttgtaagaa aggaagaacc tcaatgattg tacaagcagt
    28321 cttattagga attgttgcat ttgtcgcaca atcagaatat gcactaggga catcgttgct
    28381 ttctcgtccg attgtgacgg ggctgtttac aggaatcgtg ttaggtgatg taaaaactgg
    28441 gatcattatg ggcgcaactt tagagttagc atttatcgga tcgttctcag taggtgcttc
    28501 gattccacct gatgttgtga ccggtgggat tttagggacg gcttttgcta ttacagctgg
    28561 agccggtaca gaaaccgcgc ttttactagg tttgccgatt gctacgttga cattgatatt
    28621 gaaaaatatt tacttagggc ttttgattcc aattatgaac cataaagcag acaattatgc
    28681 agaagaagga aattataaag gagtcgagca tatgcacttg cttgccggta tcggtctttc
    28741 cttgatgctt ggtgttgtgg taatggtttc ttatatggtg ggaagcaata cgatcggaaa
    28801 tctattaaat atgattcctg acttcgttca aaaggggttg tcagtagcta cagggttgat
    28861 tccagctctt ggttttgcga tgcttgctcg tttgctgatc aacaaacaag ttgcaccata
    28921 ttttttctta ggttttgcta tagcttctta cttagagatt cctgtgaccg gagtagctat
    28981 ttttggtgcg attttagcag ttgtggtagt caatattatg aatgtccgtc aactaccact
    29041 tcaaacaact gcaggggagg tagaagaaga tgaagaattc tgagatcaaa aacagcaaaa
    29101 taacgaaaaa agagctaaat cacgtatttt ggcgttcttt tcaaatggag ttttcttgga
    29161 attatgaacg tcagatgaat atggcttatg tttatgcaat gattccgatt ttgaaaaagt
    29221 tatatcagtc gaaagaagaa atggcagcag ctttgaaacg gcatctagag ttttttaaca
    29281 cgaccccaca tatcgttaca ctgattttag gaatcacggc tgcaatggag gaagaaaata
    29341 aggatgatcc tgaatttgat gtgactgcga tcgatagtat caaaacatct ttgatgggac
    29401 ctcttgcagg gttaggagat tctttctttt ggggaacttt gcgattgatt gccacaggtg
    29461 tgggtacctc tttagccatg caaggaaata ttttaggccc tatcttgttc attttgattt
    29521 tcaatattcc gcacattctg tttcgctatc tagcaacagg ttggggatat cgcttaggaa
    29581 cgggtttttt gaaaaagatc caggcaaacg gtatgatgga aagtttgaca ttaggcgcgt
    29641 ctattattgg actgatggtc gttggagcaa tgactgccac gatgatcacc atcaatattc
    29701 ctttaaaaat cggttcagga gaaaatgctg tgactgttca aagtattttt gatgatatcg
    29761 ttccaaatat cttatgtctt ggagcatttg gcatagtgtt ttatctattg aagaaagaag
    29821 tgaaaccatt aacgatcttg atcggactag ctttctttgg aatttttggt tcgtggatag
    29881 ggatttttta aaaaaggagc agagaaaatg tttacgatgc aagattatat ctatgaagaa
    29941 aaggaagtac taagtacgat cttgaaaaag aatgattttt cgacaagaga acatatgaaa
    30001 aaaacagtga atttactgat cttagcaacg ggttcatctt ataacgcttg tttagcagcg
    30061 aagccagcat tggaatctta tggtgatctt actgtcgata ttcaagaacc tttttatttt
    30121 ttcaattatg gaaaactttc tccttcaatc gatactgtga ttgctgtgtc acaaagcggg
    30181 aaaagtgctt ctactgttga agcagtaaaa atgatacaaa aacaagggtt acctgttgtt
    30241 gctatcacaa atgacgtgca aagtccactt gctttagaag ctgcacaaat tattgattta
    30301 ggtgccgggg tagaatcagt aggcttcgtt acaaaaggct attctgcgac tgttttacag
    30361 ttactactct tagggatagg aattggaata agtaaaaata aaatcagcaa aaaaatagaa
    30421 caagactaca tgcaacagtt gcgcaaaata atcaaccatt taccagcgat tattcaaaaa
    30481 acggaagaat tttttgatgg atatcaaagt ttatttcgtt tagcacagcg tttcgtcacc
    30541 attggatatg gtccaaactg gggaacagct aaagaagcag agacgaaatt gactgaaaca
    30601 atccgcgttc cgtcacaagg atttgagtta gaagcttata tgcacggccc atacctggag
    30661 gcagatgctt cgcatttgct attctttatt gaaggggaaa gtgtaaataa ggaacgttcg
    30721 caaaagttgc aaagatatat gagtcggtac gttggagaga ctctcaccat taccacaaaa
    30781 aaagcaagaa acgagaaaac acttggatta gcaatagaat gtgatgaata tctgtctgta
    30841 ttagcgcttg tcgttccgtt tcaactattc gcttataaaa ccgcagtagc taaaggtatt
    30901 gacttgaaca aaaagatttt tgaagacttt gatacagtct tgaaaagtaa attataagag
    30961 gtggaaatca tgctgaaatt caatgaagaa gaacaaatca aagcaataaa aggcgcacta
    31021 gctttacgtc cgcaagtaga agaaatcatc gataaacttt atgcggaaaa atttgacgct
    31081 gtctattatc taggaatcgg tggaacgtat gcttcctcga tgcaagctgt cacttatatg
    31141 aatgggaaaa gcaacttgcc tgtctttgta cagcatgctg cagaatatta cactactgga
    31201 aacaaaagat taacaaaaga ttctatcgtt attttgtcct ctgttaccgg tacaactcaa
    31261 gaagtagtga aagcagtaga acaaatcaag aaagtaggag cgacactgat tggatttatc
    31321 gataaagcga acagtaagtt gtctcagcta tgcgattttg tggtcactta tccagctcct
    31381 ggaacagaac aaatcaaatt ttttatggca gcagatcgat tgatgtattt gaatggagaa
    31441 ttcgaagctt attcagaata ctatgaacag ctcgaacgtt atttaccaac agggttagtg
    31501 gaagcagaga aaaaagcaga tgcatttggc ttatcatttg cagaaaaaca tcgacgtgat
    31561 agtatgcatt attttattgg agcagggaat caatggggag ctgtttattc atatgccatg
    31621 tgttactggg aagaacaaag ctggcttcca tcgaaatcta tccatgctgc cgagtttctt
    31681 cacgggacgt tagagatcgt agaagaaaca accccagtca cattattttt aggcgaagat
    31741 gaacagagag tgttatctga acgtgtggct aaacttttgc caaaaatttg cagcaattat
    31801 acattgatag acacaaaaga ctatccagta gaaggtatca gtgaaaaata tcgcggacgt
    31861 gttttgtctt tcttattgat gcatgtagtg acacaacgca tcgatgctca tgtggagcag
    31921 ttgaactgcc atccattaga gatacgccgc tactatcgtc agtttgatta ttaggataga
    31981 ctggataaaa agaatataaa aaataaagag agcagcgata actgctccct ttattttttt
    32041 aaaatattgg catcaattac gttataaaac acattttttg cattatctac agttcaactt
    32101 ataatctgtt taatgggaca tgtgggtgcc aaccattttt tgttatgcaa tagtactatt
    32161 tagttgttgc atggatccgt agtaaaagtc caagtacaac gagttgcgcc agtttacata
    32221 gcgttttgta tccagaatgt tgactaaaac aaaagttcct atgtcaaccg tcaacagaga
    32281 caatctgtcc gttgcaggac ctgacagatt tagaaatgtt caatctgtct cttatgtatt
    32341 ttaaatcaat actcactagg agaagtttcc taacctccta atagtaatgc tggtagtaac
    32401 attgtcaaaa ctgcttcctc ttaggaacaa gcaaagcata acgagtgtaa aaataaaatg
    32461 tagactattg taacatatca ttgattattt caagtaaaaa cgataaaaga agtaaacaaa
    32521 cacttaaaaa caatggaaat tagtcccaac tttaagaaat gtatactata atggaaatta
    32581 tcaattattt atttggagga atatccatga aacaaaaaat cgcagttctt ggtcctggat
    32641 cttggggcac tgctttagca caaacattgg cagaaaacgg gcatgatgtc cgtatttggg
    32701 gaaatgtacc tgaacagatt gatgaaatca ataagtatca tacaaatcag cattttttac
    32761 ctgatttgac gatcccagaa tcgattatcg gatataaaga attggcagaa gcagtggata
    32821 acgcagatgc tattttattt gttgttccaa caaaagcgat tcgttcagtc gcacaggaac
    32881 ttattggtaa aatgaatact aagccaatca ttatacatgc aagtaaagga ctagaacaag
    32941 atacgcataa acgaatctca gaagttttgg ctgaagaaat tccagaagaa aaaagacagg
    33001 caattgttgt attatctggt cctagccatg ctgaggaagt ggcagtacat gatatcacaa
    33061 caattacagc tgcaagtatt gatcaaaaag cagcagccta tgtacaaaaa ctgttcatga
    33121 atgattattt caggatttat acaaataatg atgtgattgg cgtagaaact ggtgcagcac
    33181 tgaaaaatat cattgcaatt ggtgccggcg ctatccatgg attaggattc ggagatgatg
    33241 caaaagcagc aatcatgact cgaggattgg cagaaatcag ccgtcttggt gtcgcgatgg
    33301 gtgccaatcc tctgacattt atcggtttga gtggtgtggg ggacttgatc gttacttgta
    33361 caagtgttca ttctcgcaat tggcgtgccg gtaacttgtt aggacaggga cataagctgg
    33421 aagaagttct agaaaacatg ggaatggtcg ttgagggtgt ggcaacgaca aaagcagcag
    33481 tagagctagc tcaacagcta ggtgttgaga tgccaattac tcaaacaatc tataatgttt
    33541 tgtataatgg agaagatatc aaaaaagcag caaaagaaat catgttacgt gacggaaaaa
    33601 tggagaatga attttctctt cgttaaatag atgatcaata gttaatcgt
//
LOCUS       DACDKM010000019        32009 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20834_2016
            SAMN12640370-rid9891663.denovo.019, whole genome shotgun sequence.
ACCESSION   DACDKM010000019 DACDKM010000000
VERSION     DACDKM010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640370
            Sequence Read Archive: SRR10040891
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 32009)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32009)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742835.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742835.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:04:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,012
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 29 of 75
            Pseudo Genes (incomplete)         :: 33 of 75
            Pseudo Genes (internal stop)      :: 19 of 75
            Pseudo Genes (multiple problems)  :: 5 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32009
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640370-rid9891663.denovo.019"
                     /isolate="E20834_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            128..1396
                     /gene="rseP"
                     /locus_tag="IV334_000913"
     CDS             128..1396
                     /gene="rseP"
                     /locus_tag="IV334_000913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HAP8943004.1"
                     /translation="MKTILTFIIVFGILVIVHEFGHFFFAKRSGILVREFAIGMGPKI
                     YGHQAKDGTTYTLRLLPIGGYVRMAGNGDDETEMAPGMPLSLLLNSDGIVEKINLSKK
                     IQLTNAIPMELSRYDLEDELTITGYVNGDETEVVTYPVDHDATIIENDGTEIRIAPKD
                     VQFQSAKLWQRMLTNFAGPMNNFILAILLFIILAFMQGGVQVTNTNRVGEIMPNGAAA
                     EAGLKENDEVVSVDGKEIHSWNDLTTVITKNPDKTLDFKIEREGQVQSVDVTPKSVES
                     NGEKVGQLGIKAPMNTGFMDKIIGGTRQAFSGSLEIFKALGSLFTGFSLDKLGGPVMM
                     YQLSSEAANQGVTTVISLMALLSMNLGIVNLLPIPALDGGKLVLNIFEGIRGKPLSQE
                     KEGILTLAGFGFLMLLMVLVTWNDIQRFFF"
     gene            1464..3173
                     /locus_tag="IV334_000914"
     CDS             1464..3173
                     /locus_tag="IV334_000914"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HAP8943005.1"
                     /translation="MRQSKMLIPTLREVPNDAEVLSHQILLRAGYIRQVSAGIYSYLP
                     LANRVLEKLKRIMREEFEKIGAVEMLMPAILPAELWEESGRYETYGPNLYRFKDRNDR
                     KMILGPTHEETFTDLMRNEINSYKKLPLNLYQIQPKYRDEKRPRFGLLRGREFIMKDA
                     YSFHATEESLDETYKDYEKAYTEVFKRCGLEFRSIIGDGGAMGGKDSKEFMAISEIGE
                     DTICYSTESDYAANLEMATSYYVSKKSHETQLELEKVATPNVKSIDEVADFFSVEPQK
                     IIKSVLFIADEQPVLVLVRGDHEVNDVKLKNFLSADFLEEATEEEAQKYLGAEFGSVG
                     PVGVSDDVKVYADRYVQDLANAITGANETGFHYKNVNPERDFTVLSYEDLRFVQEGDP
                     SPDNNGVLAFTRGIEIGHIFKIGTRYSESMGATVLDENGREKFVIMGCYGIGVSRLLS
                     AIVEQHSDEQGINWPKGIAPFDLHIVQMNLKDEYQTNLTNELEEAMTKAGYEVLVDDR
                     NERAGVKFADSDLIGCPIRITVGKKAVDGIVEVKIKKTGEMVEVRKEELVNTLPILLN
                     QGE"
     gene            3385..7743
                     /locus_tag="IV334_000915"
     CDS             3385..7743
                     /locus_tag="IV334_000915"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PolC-type DNA polymerase III"
                     /protein_id="HAP8943006.1"
                     /translation="MSEKRDLFEKLLEQIQLEEAEKNHPLLTAGEMDSVVVHRKSRLW
                     EFTLHFSKILPIMLYRSLTQHLTIAFKDIAQVKLNILADDQTFDEQLLQDYWAQALEN
                     QQCDTPLAQQVLKTQVPVIKDRKVILPIDSTGAISYLKQQYLPLVEELFVSYGFPKFR
                     IEPEVDEQQAERVLKLFEERKQEQAEAFMKQAAESLVVHEQKKKERKEQSPALEGPIH
                     IQLGRNIPADEPTTPMISIVEEERRVTLEGYVFDKEVRELRSKRKILTLKITDYTSSF
                     IVKKFSNGEKDEQVFDAISVGSWLKVRGSIQEDTFVRDLVMNAQDIIEVKHTPRKDYA
                     PEGEKRVELHVHSNMSTMDATNSISDLVAQAGKWGHRAIAITDHGGAQAFPEAHSAGK
                     KAGVKILYGVEANVVDDGVPIAYNDAHEALSEATYVVFDVETTGLSAVYDTIIELAAV
                     KMYKGNVIESFDEFIDPGHPLSRTTVDLTGITDGMVRGSKSEEEVLRMFLEFSKDTIL
                     VAHNAAFDMGFLNTSYARYGIPEAANPVIDTLELARYLYPQFKRFGLGVLSKKFGVSL
                     EQHHRAIYDAEATGHLAWIFVKEAMDNHNMLYHDQLNEHIGEGDSYKRARPFHVTILA
                     KNQAGLKDLFKLISMSNVEYFERVPRIPRSQLKKMRENLLIGSACDKGEIFEAMMQKG
                     VEEARNRAKFYDYIEVMPKAVYAPLIEQELVKNEHDLEEIIQNLVEIGKSLDKIVVAT
                     GNVHYLNEEDAIYRKILINSMGGANPLNRHSLPDVHFRTTDEMLTAFHFLGEETAKEI
                     VVENTNKIADICEEVIPVKDELYTPKIPGSEDEISELSYTKAKQMYGDPLPEIIQKRL
                     KKELNSINGNGFSVIYLIAQKLVHKSNEDGYLVGSRGSVGSSFVATMTGITEVNPLAP
                     HYYCPECQYSEFFEDGTYGSGFDMPEKQCPKCGARLNKDGHDIPFETFLGFHGDKVPD
                     IDLNFSGDYQAEAHNYTKVLFGEDYVYRAGTIGTVADKTAYGYVKGYERDNNLQFRSA
                     EVDRLAKGATGVKRTTGQHPGGIIVIPDYMDVYDFTPIQYPADDQNSEWKTTHFDFHS
                     IHDNVLKLDILGHDDPTVIRMLQDLSGIDPQTIPTDDPEVMRIFAGPEVLGVSQEQIY
                     SKTGTLGIPEFGTRFVRGMLEETHPTTFAELLQISGLSHGTDVWLGNAEELIRRGDAT
                     LAEVIGCRDDIMVYLIHAGLDSGMAFKIMETVRKGQWNKIPDELRETYLSAMKENNVP
                     DWYIDSCSKIKYMFPKAHAAAYVLMALRVAYFKVYFPILYYCAYFSVRADDFDLVSMC
                     KGKDAVKQAMKEITDKGLDASVKEKNQLTVLELANEMLERGFKFGMIDLYKSDAVNFV
                     IEGDTLIAPFRAVPSLGTNVAKQIVEARKDGPFLSKEDLATRGKVSKTLIEYMNDNGV
                     LKDLPDENQLSLFDML"
     gene            7888..8361
                     /gene="rimP"
                     /locus_tag="IV334_000916"
     CDS             7888..8361
                     /gene="rimP"
                     /locus_tag="IV334_000916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HAP8943007.1"
                     /translation="MSSVVETVTEMVTPILDEQNFELVEVEFVKEGKSWFLRVFIDKE
                     GGIDIEECAFVSEKLSEKLDAADPDPIPQAYFLEVSSPGAERPLKKESDYEKALGDYV
                     HVSLYQPVDGEKQYEGFLQSFDAEQLTLKIRIKTREKELTFDRKNIAKARLAIQF"
     gene            8384..9559
                     /gene="nusA"
                     /locus_tag="IV334_000917"
     CDS             8384..9559
                     /gene="nusA"
                     /locus_tag="IV334_000917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusA"
                     /protein_id="HAP8943008.1"
                     /translation="MSKEMLNALDALEAEKGISKEIVIDALEAALVSAYKRHYGQAQN
                     VEVEFDQKKGKIHVYAVKEVTEEVMDSQLEVSLKDALLINPAYEIGDTIRFEVTPKDF
                     GRIAAQTAKQVILQRVREAERTIIYNEFSAYEKDIMQGIVERQDKRYIYVNLGKIEAV
                     LSKQDQMPNEFYQPHDRIKVYVSRVENTSKGPQVFVSRSHPDLLRRLFEQEVPEVYDG
                     IVEIVSIAREAGDRSKVAVRSNDPNIDAVGTCVGPKGQRVQAIVNELKGENMDIVEWN
                     EDPAVFIANALNPAQVMDVIFDETNPKACTVVVPDYQLSLAIGKRGQNARLAAKLTNH
                     KIDIKSESDMTEFYENQEQQKETEELHDEAIVQSDLTDDEYETIAFNNETVEEKPEA"
     gene            9581..9874
                     /locus_tag="IV334_000918"
     CDS             9581..9874
                     /locus_tag="IV334_000918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxR family protein"
                     /protein_id="HAP8943009.1"
                     /translation="MKQRKIPLRKSVVSGEMKPKKEMVRITRSKEGEVSIDPTGKMPG
                     RGAYVSLEPEEVQQAWDKKILDRVLETTLTDEFYQELLDYVTHQKARKELFGK"
     gene            9871..10182
                     /locus_tag="IV334_000919"
     CDS             9871..10182
                     /locus_tag="IV334_000919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxQ-related RNA-binding protein"
                     /protein_id="HAP8943010.1"
                     /translation="MNTVNRQKAMNLIGLAMRAGKLITGEELTIAEIRKQKAKLVFVA
                     TDASENTRKKIKDKSSYYEVPCFELFSEAEITQMIGKPRKVFGIMDAGFAKKTKELIE
                     G"
     gene            10195..12501
                     /gene="infB"
                     /locus_tag="IV334_000920"
     CDS             10195..12501
                     /gene="infB"
                     /locus_tag="IV334_000920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HAP8943011.1"
                     /translation="MGNKRIYELAKELKKSSKDVVEKAQQLGIDVKNHMGAITSNDEK
                     KLQQAFKNPSTKQQAAQQASRKPANQQPNDQRKKETTNNQQKNKNNRSYQDRGQGSGQ
                     VNQGKKPTTNQNNTGNTSSQNRQGTSQGGNQNRTNNNNKPNNNNRGKFNNNNRNRYNK
                     KGKKGKQQQSTKPAVPPRKFRELPDVLEYTEGMNVADIAKKIHREPAEIIKKLFMLGV
                     MVNQNQALDKDTIELLATDYGMEPQEKVQVDIADIDKFFEAEEVDPDKLVSRPPVVTI
                     MGHVDHGKTTLLDTLRHSRVTSGEAGGITQHIGAYQIDIDGKPITFLDTPGHAAFTSM
                     RARGASITDITILVVAADDGVMPQTVEAINHAKAAGVPIIVAVNKIDKPGANPQHVMQ
                     ELSEYELIPEAWGGETIFVEISAKFGQNIEELLEMILLVAEVEDLKADPTQRAIGTVI
                     EARLDKGKGPVSTLLVQEGTLHVGDPIVVGNTYGRVRVMTNDLGRRDKEAGPATPVEI
                     TGLNDVPQAGDRFVVFEDEKTARAAGEERAKRALLENRAATSRVTLDNLFESLKEGEL
                     KEVNVIIKADVQGSAEALAASLKKIDVEGVRVKIVHSAVGAINESDVTLAAASNAIII
                     GFNVRPTPQAKVQADTEEVDIRLHRIIYKAIEEIETAMKGMLDPEFEEKITGQMIVRE
                     TFKVSKVGTIAGAYVTEGYIRRDSGVRVIRDGIVIYEGQLASLKRFKDDVKEVKMGYE
                     CGAMIEKFNDIKVDDVIEGFIMEEVKND"
     gene            12528..12875
                     /gene="rbfA"
                     /locus_tag="IV334_000921"
     CDS             12528..12875
                     /gene="rbfA"
                     /locus_tag="IV334_000921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HAP8943012.1"
                     /translation="MANYRDRRVGQEILKEVNDILRKKVRDPRVENVTITDVKVTGDL
                     QQATIYYSILSDLASDKKTAQDGLNKASGLIRRELGHNLSIYKTPELFFELDESVVYG
                     NHIDELLRDLNKD"
     gene            complement(13032..13205)
                     /locus_tag="IV334_000922"
     CDS             complement(13032..13205)
                     /locus_tag="IV334_000922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP8943013.1"
                     /translation="MIFLLTVNGPTNSNFYGAGSTDLLVTWLYLTVTKIDYNLASVIG
                     ILIFIYMQKFIYY"
     gene            13653..16015
                     /locus_tag="IV334_000923"
                     /pseudo
     CDS             13653..16015
                     /locus_tag="IV334_000923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625383.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoketolase family protein"
     gene            16383..17306
                     /gene="truB"
                     /locus_tag="IV334_000924"
     CDS             16383..17306
                     /gene="truB"
                     /locus_tag="IV334_000924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HAP8943014.1"
                     /translation="MDGLLPLWKERGMTSHDCVFKLRKILHTKKIGHGGTLDPDVDGV
                     LPICIGKATKVIEYLTDSGKIYVGEITLGFSTTTEDRSGERVETKPVDMSLSEKKIDE
                     VMASFIGEITQIPPMYSAVKVNGKRLYEYARNNETVERPVRKAQIYRFIRTSELTRDE
                     VTGTISWRFEVECGKGTYVRTLAVDTGKKLGYPAHMSDLTRTASAGMSQKDALTLEEV
                     AQAVKNGTIDDCLLPIEKGVERFKRVDITDEVWQKVKNGMRLDYTVFALPEMPSEEIA
                     LFYKGKVVSIYQPNPKEKNKLKPSKVLRNEE"
     gene            17310..18251
                     /gene="ribF"
                     /locus_tag="IV334_000925"
     CDS             17310..18251
                     /gene="ribF"
                     /locus_tag="IV334_000925"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin biosynthesis protein RibF"
                     /protein_id="HAP8943015.1"
                     /translation="MNIIKIRHPYQSEQIPEDDVVLVLGFFDGVHIGHQKVIKTGMEI
                     AQKEGLKLALMTFNQHPSIVFKKIDPSSVKYLTSLKQKEEKMAALGIDYLYEVDFTSS
                     FAHLAPQEFVDQYIVGLHAKYAVSGFDYTYGPKDIADVAHFPEYAKGRFKIVTVSKEE
                     EQGEKISSTRIRGLLDSGQVKEAAKLLGCTYEVEGVVVHGDARGRLLGFPTANVKVKS
                     TVHLPKEGVYVSEIKIGETWYPAMGSIGHNDTFGEGRELTVELYILDFNQDIYGEHVA
                     VRWNQFLRDQVKFSSVEELIEQLKEDEQATAEYFSQQ"
     gene            18603..19388
                     /locus_tag="IV334_000926"
     CDS             18603..19388
                     /locus_tag="IV334_000926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAP8943016.1"
                     /translation="MYKELENKVAVVTGGSKGIGTAIAQRFGKEKMNVVVNYNSDKEG
                     AEKAVKAIEEAGGKAVSVQADVSSEEGIQRLLDTALENFGTLDVWVNNAGMENQHPTH
                     ELSLEDWERVLKVNLTGVFIGTKAALNYFTENNKKGNIINMSSVHEQIPWPTFAHYAA
                     SKGGVKLFTETVAMEYAPKGIRINSIGPGAIRTPINAAKFDDPEQKELLESMIPLGRV
                     GEPKEIAATAAWLASDESSYVTGITLFADGGMTLYPSFQGGKG"
     gene            19401..20252
                     /locus_tag="IV334_000927"
     CDS             19401..20252
                     /locus_tag="IV334_000927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transporter"
                     /protein_id="HAP8943017.1"
                     /translation="MGLLIALIPAVGWGIQPLILKKIGGRPTNEILGTGIGAVLVGLL
                     VQLFASPAGISVTTFLISLLSGAFWVIGQTGQYTSFNLIGVSKTMPISTALQLVGTSL
                     IGVFAFGEWSGSMGKIIGAAAIILLVFGSALTAISDDPGKKGGITKGISLLASTSIGY
                     WVYSALPKLVDASGLSIFFPQMIGVFLGAVIYVIFRKPTAFSEDKSWKATIVGAIFSV
                     SALAYIFSANENGVATAYIITQLNVVISTLGGMYILHENKTPKEKKLTIIGLLLIVVG
                     SMVTVFI"
     gene            complement(20376..20828)
                     /locus_tag="IV334_000928"
     CDS             complement(20376..20828)
                     /locus_tag="IV334_000928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP8943018.1"
                     /translation="MKDFYSVNELAEQLGVTTRSIRNYLHEGKLKGTKVGGQWKFSER
                     NLFEFLYGDQAEEAAKEMQRFMLNAPITMRFNLQYRDFTAINQFREQLVQYHNDVYAN
                     KKDRLLQYDLYKDNHAEILIGGNFNYVVNFSQWINEKLLIQTDISLVS"
     gene            complement(21293..21661)
                     /locus_tag="IV334_000929"
     CDS             complement(21293..21661)
                     /locus_tag="IV334_000929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chaperone"
                     /protein_id="HAP8943019.1"
                     /translation="MEKFQPIINQIKEKEHQQKIKDLFAKITETYPQLDTTVKWNQPM
                     FTDHGTFIIAFSFAKNHFSIAPEKAAIRALEKNIQEAGYVYTDNVIKVPWKSVINWEL
                     IEQLISFNIEDKKGHDKFWR"
     gene            21811..22293
                     /locus_tag="IV334_000930"
     CDS             21811..22293
                     /locus_tag="IV334_000930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl-tRNA editing protein"
                     /protein_id="HAP8943020.1"
                     /translation="MIDLAKKFLHENIYFELYHHRAIYTNEDALVIKKEQGFTGTETK
                     SLFLKDKKDNNYVYLTFTTKQTDFKALGKIVGKRLSIVSSERMEEQTGQKQGAVSPFG
                     YEKQIPIIIDEELLSNEKLVFAPGRPDQTMVIQTVDLNKIIQLLEMPIFLMPANEKNN
                     "
     gene            22330..23340
                     /locus_tag="IV334_000931"
     CDS             22330..23340
                     /locus_tag="IV334_000931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LD-carboxypeptidase"
                     /protein_id="HAP8943021.1"
                     /translation="MISYPLLERNATIGVTAPSSGVSEEHHSLLESALKRMEEKSFQV
                     VTGKTLWTQDKEKSGPARKRADEFNQMMQDENIQMIFPTWGGELLIEILDFVDFDALQ
                     PKWIVGYSDISTLLLAVTLKTGIATAHGTNLIDLRGEYLDETTAMWESVLKTREGESI
                     LQQSSEKYQKVWPHEHSSSSVFHLTEPTNWQSVSGDAVRMKGRLLGGCIDTIRHLIGT
                     PYGDVQSFKRKYNDGKPILWFLENCELSSVDLRRSLVQMKLAGWFDGSSGIMFGRSAA
                     EDLLDYKMKDVYEDLSKELKLPILYDIDCGHVPPQLTLINGAYAEVAVEDRKATVLQT
                     FI"
     gene            complement(23455..24168)
                     /locus_tag="IV334_000932"
     CDS             complement(23455..24168)
                     /locus_tag="IV334_000932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8943022.1"
                     /translation="MKKLGFILLLVSLFLVGCTSPSKENSDPSATTDKSSIRQTSSAE
                     KSSTQKSASTETNTSSTSSKVSEPNTSSTSSSTVETNISSSTIDTTSSQSSQSIDPLA
                     GYSDLQIEYARVWLAIRGNTFRDTSGDFELHVIHYPAGTPIAPYDAGSAVYPKDVVML
                     TGKYSYQGVLVYSSNHDGTITSYPVPSHWQMPADQTSYPAFIRETTQEIIDNASVVAI
                     PTGNPNDVKQLIDVTVIDN"
     gene            complement(24358..24948)
                     /locus_tag="IV334_000933"
     CDS             complement(24358..24948)
                     /locus_tag="IV334_000933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine amidotransferase"
                     /protein_id="HAP8943023.1"
                     /translation="MKTAITFLLEEYADWEGAYITSTLNQNEKWQNKTASIQSEVTSI
                     GGLKTKIDYLIEQIPEKVDLFIMIGGNSWDIKNHHLYQIIDKLLKTDVPVAAICGAVD
                     YLAQSGLLQGYKHTGNAQFPWLESENYQNKEDFIEQQAVKDRNLITANGTAPIEFTDL
                     ILQAVHFETEEIREKTIRLYKLGFYDYYKKYGDPFS"
     gene            25432..25659
                     /locus_tag="IV334_000934"
     CDS             25432..25659
                     /locus_tag="IV334_000934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HAP8943024.1"
                     /translation="MKIAFHIFADFDLMPKVSFAITVYREAVKAPCSSDDNKQAFLEL
                     GFLDSKYAAYFNELVLERDFKKVENRHDFST"
     gene            25675..26841
                     /locus_tag="IV334_000935"
     CDS             25675..26841
                     /locus_tag="IV334_000935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HAP8943025.1"
                     /translation="MIETINPAENKVSAYIHIPFCEHICYYCDFNKVFLEGQPVDEYV
                     EMLLNEMKIMLESHPTANLETLYVGGGTPTSLSAKQLDRLLSGAREILPFQNGNEFTV
                     EANPGDLTREKLQVMKNYGVNRLSMGVQTFDNRLLKKIGRKHTAEDVYQTMQFLEAEN
                     FTNVSIDLIYALPGQTLEGYLDTLEKALALDLPHYSLYSLILENKTMFMNWVRQGRLE
                     LPDQETETRMFEETIEAMEKHGRHQYEISNFGLSGHESQHNLMYWNNDHYFGFGAGAS
                     GYLGDIRYRNKGPIQHYLRPLRANERPVLEEEKLTRKNQIEEEMFLGLRKKTGITKKH
                     FYDRYQKTIESVYGDVLRELEQEGLLVNEEDRVYLTPRGTFLGNNVFERFLFEK"
     gene            27075..28118
                     /gene="hrcA"
                     /locus_tag="IV334_000936"
     CDS             27075..28118
                     /gene="hrcA"
                     /locus_tag="IV334_000936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HAP8943026.1"
                     /translation="MLTKRQNDILRLIIQHYTSSGVPVGSKTLMSEGVKASSATIRND
                     MKALEDEGLLLKTHSSSGRIPSALGYRYYVDHLLKPARVAEDDLLLIRQSLGREFHEI
                     NEIIKQSAEILSELTSYTTFSLGPEIKDRRLTGFRIVPLNNRQIIAIVVTDMGNVESQ
                     VFTLPEYMGSQELEKMVQIVNDRLVGDPLMTVYHKLRTEIPMILHKYFQTTEGMSQLF
                     DTVLGHVFEDKVYVSGQMNLLDYEPRQDLDQFKSMFSFMKDSDGLTQMITPMDSNIHI
                     KIGAELGNDLLQNMSMIQASYEIDGHGRGTIALLGPTSMPYSRMFGLVDVYRRELAAK
                     LADYYRSLDLSNS"
     gene            28212..28775
                     /gene="grpE"
                     /locus_tag="IV334_000937"
     CDS             28212..28775
                     /gene="grpE"
                     /locus_tag="IV334_000937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HAP8943027.1"
                     /translation="MKENQEELDKEMTDAQPEPEIDVEAAEDSGISEAEAEEFETAKL
                     KAELEEMEDKYLRARAEIANMANRGKNEREQLQKYRSQDLAKKLLPSIDNLERALATE
                     VSDDQGAGLKKGVEMVLESLRNALEEEGIEKIPAKGEAFDPNLHQAVQTVPATEDTPA
                     DTIVEVLQEGYKLHDRVLRPTMVIVAQ"
     gene            28826..30655
                     /gene="dnaK"
                     /locus_tag="IV334_000938"
     CDS             28826..30655
                     /gene="dnaK"
                     /locus_tag="IV334_000938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016635026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HAP8943028.1"
                     /translation="MSKIIGIDLGTTNSAVAVLEGNEAKIIANPEGNRTTPSVVSFKN
                     GEIQVGEVAKRQAVTNPNTISSIKRHIGEAGYKVDVEGKSYTPQEISAMILQYIKGFA
                     EDYLGEKVEKAVITVPAYFNDAQRQATKDAGKIAGLEVERIVNEPTAAALAYGLDKTD
                     RDEKILVFDLGGGTFDVSILELGDGVFDVLSTAGDNHLGGDDFDNKIIDYMVAEFKKE
                     NSIDLSQDKMALQRLKDAAEKAKKDLSGVSSTQISLPFITAGESGPLHLEMTLTRAKF
                     DELTADLVERTKVPVRQALKDAGLSQSEIDEVILVGGSTRIPAVVEAVRKETGKEPNK
                     SVNPDEVVAMGAAIQGGVITGDVKDVVLLDVTPLSLGIETMGGVFTKLIDRNTTIPTS
                     KSQVFSTAADNQPAVDIHVLQGERPMAADNKTLGRFQLTDIPAAPRGIPQIEVTFDID
                     KNGIVNVSAKDLGTQKEQKITIKSSSGLTDEEIERMVKDAEANAEADKARKEEVDLRN
                     DVDALLFSVDKTLKELEGKVDEEEVKKAETARDELKAAVEANNIEEMKTKRDALNEIV
                     QNLTVKLYEQAAQQQAQENPEAAQGGADDVVDADFEEVDGDDK"
     gene            30806..31972
                     /gene="dnaJ"
                     /locus_tag="IV334_000939"
     CDS             30806..31972
                     /gene="dnaJ"
                     /locus_tag="IV334_000939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010767443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HAP8943029.1"
                     /translation="MATKRDYYEVLGLSKGASEDEIKKAYRKLSKKYHPDINKEPDAE
                     EKFKEVSEAYEILSDPQKRAAYDQYGHAGTDPNYGAGGAGGGFGGFGGFSGGGFGGFE
                     DIFDSFFGGGGRSVDPNAPRQGADLQYTVDLSFEEAIFGVEKEIKYNREEICHTCGGN
                     GAKPGTQPTTCHKCHGAGTINVERQTPLGRVMSRQTCDVCHGTGKEIKEPCPTCHGSG
                     HEKKAHSVKVNVPAGVEEGQQMRLAGQGEAGENGGPFGDLYVVFRVEESDIFDREGSE
                     IYYELPLSFVQAALGDEVQVPTVHGDVKLKIPAGTQTGTNFRLRGKGAPKLRGGATGD
                     QHVKVKLITPKNLNEQQREALRAFAEAGGIKVEEQQEDGFFDKFKDAFGGKKRK"
BASE COUNT        10945 a         5544 c         6884 g         8636 t
ORIGIN      
        1 aaagacgcag gtttttattt ttcattatat tgatactatt ggtgtctttg gtatactatg
       61 gtaaaattat agtgttttat cgttgtttta taaaaaacac aaaaaacaag taaaggagaa
      121 aaaagctatg aaaacgattc tgacatttat catcgttttt ggtatattag tgattgttca
      181 tgagtttggt catttcttct ttgcgaaacg atcaggaatc ctcgttcgcg aatttgctat
      241 cggtatgggt ccgaaaattt atgggcatca agcaaaagac gggacaacat atacgcttcg
      301 cctattacct attggcggtt atgttcgaat ggcaggcaat ggggatgatg aaacagaaat
      361 ggcaccagga atgcctcttt ctcttttact aaattctgac ggcatcgtag agaagataaa
      421 tttaagtaag aaaatccaat tgacaaatgc tatcccgatg gaattgagcc gttatgattt
      481 agaagatgag ctgactatca ctggttatgt gaatggagac gaaacagaag tagtaaccta
      541 ccctgttgat cacgatgcga cgattattga aaacgacgga acagaaatca gaattgcgcc
      601 aaaagacgta cagttccaat cggctaaatt gtggcagcgt atgctgacca actttgctgg
      661 acccatgaat aactttattt tggcaatcct gttattcatc attctggcat ttatgcaagg
      721 tggtgttcaa gtgacgaaca ctaatcgcgt aggagagatc atgcctaatg gagctgcggc
      781 agaagcaggc ttaaaagaaa atgatgaggt agtcagtgtg gacggaaaag aaatccattc
      841 atggaatgat ctgacaacag tcattacgaa aaatccggat aaaacattag actttaagat
      901 cgaacgagaa ggtcaggtgc aatctgtcga cgtgacaccg aaatctgtag agtcaaatgg
      961 cgaaaaagtt ggccaacttg gtataaaagc cccaatgaat acaggtttta tggataagat
     1021 cattggaggg acgcgtcaag ctttcagcgg ttcattggaa attttcaaag ccttaggatc
     1081 actatttaca ggtttcagtt tagataaatt aggcggacct gtcatgatgt accaattgtc
     1141 ttcagaagca gcaaatcaag gtgtaacaac tgttattagt ttgatggcgc ttctttcaat
     1201 gaatctcgga atcgtcaatc tgcttccgat tcctgcctta gatggcggga aattagtctt
     1261 gaatattttt gaagggattc gcgggaaacc attaagtcaa gaaaaagaag gaatattgac
     1321 attagctggc tttggatttt taatgttgtt gatggtgcta gttacatgga acgatattca
     1381 acgattcttt ttctagaaaa agttgacaaa tagggtataa taacaaacga taactatttt
     1441 gaggaaataa aggagtatat agaatgagac aatcaaaaat gttgattcca acattacgcg
     1501 aagttccaaa tgatgcagaa gtattaagcc atcaaatctt gctaagagct ggatatatcc
     1561 gccaagtatc agcgggtatt tattcatatc tgccattagc aaatcgtgta ttggaaaaac
     1621 tgaaacgcat tatgcgggaa gaatttgaaa aaatcggagc ggtagaaatg ctgatgcctg
     1681 ccatcttgcc agcagaactt tgggaagaat ccgggcgtta cgaaacatat ggaccaaact
     1741 tgtatcgttt caaagaccgc aatgatcgaa aaatgatcct tggaccaact cacgaagaaa
     1801 cttttactga tttgatgcgt aatgaaatca attcatataa gaaattacct ttaaatcttt
     1861 atcaaatcca acctaaatat cgtgacgaaa aacgacctcg cttcggtttg cttcgcggaa
     1921 gagagtttat catgaaagat gcgtattctt tccatgcaac ggaagaaagt ttggacgaga
     1981 catataaaga ttacgaaaaa gcctacaccg aagtattcaa gcgttgtggc ttagaatttc
     2041 gctcgattat cggagacggt ggcgcaatgg gcgggaaaga ctcaaaagaa ttcatggcga
     2101 tctcagaaat cggggaggat acgatctgtt attcgactga aagtgattat gctgcaaact
     2161 tggagatggc tacaagctac tatgtatcaa aaaaatcgca tgaaacacag cttgaattgg
     2221 agaaagtagc tacacctaac gtaaaatcaa ttgacgaagt agcagatttc ttttctgttg
     2281 aacctcaaaa aatcatcaag tctgtattgt tcattgcgga tgaacaacct gtccttgttt
     2341 tggtacgcgg agaccatgaa gtcaatgacg taaaactgaa aaacttttta tctgctgact
     2401 ttttagaaga agcaacagaa gaagaagcac aaaaatatct aggtgctgag tttggttctg
     2461 tcggaccagt cggtgtttca gatgacgtaa aagtatatgc agaccgttat gtccaagatt
     2521 tagcgaatgc cattactgga gcgaacgaaa caggattcca ctataaaaat gtgaatccag
     2581 aacgcgattt cactgtactt tcttatgaag atcttcgttt tgtacaagaa ggagatcctt
     2641 caccagacaa caatggtgtt cttgccttta ctcgaggaat cgaaatcgga catatcttca
     2701 aaatcggcac tcgttacagt gaatctatgg gagcaactgt actagacgaa aatggacgag
     2761 aaaaatttgt gattatgggc tgttatggta tcggtgtaag tcgtttatta tcagcaatcg
     2821 tagaacaaca ttcagatgaa caaggaatca actggccaaa aggaattgcg ccatttgatt
     2881 tgcatatcgt ccaaatgaat ctaaaagacg aataccaaac aaacctgacg aatgaactag
     2941 aagaagcgat gacaaaagct ggctatgaag tactggttga tgacagaaat gaacgcgcag
     3001 gagtgaaatt cgcggattct gatttgattg gctgtccaat cagaatcact gtcggtaaaa
     3061 aagcagtgga tggaatcgta gaagtgaaaa tcaagaaaac tggcgaaatg gttgaagtac
     3121 gtaaagaaga attggtgaat actttaccaa tcctcttgaa ccaaggcgaa taataagcgt
     3181 taaaacacag aaaataaaaa tagctgaatc agctgttttt gacaatctga tgattcgctt
     3241 ttaaaattgg aactgcgatt tgaatcgcag ttccaatttt ttaatgtaaa actcagaaaa
     3301 aacgagtata ctaaaaggta ctgaaaaaga agaggataag atgcgatcaa ttgcattgtt
     3361 tctcatcaga aagggcgtat atctttgtca gaaaaacgag atttatttga aaaactgttg
     3421 gaacaaatcc aactagagga agcagaaaaa aatcatccac tgcttactgc tggtgaaatg
     3481 gatagtgtag tggtccatcg taaatcgcgt ttatgggaat ttacgctgca tttttccaaa
     3541 attttgccta ttatgctata tcggtcattg actcagcatc tgactatcgc ttttaaagat
     3601 attgcacaag tcaaactgaa tatcttagca gatgaccaga cattcgatga acagctcttg
     3661 caagattact gggcccaagc tttggaaaat cagcaatgcg atacaccact agcacagcaa
     3721 gtattgaaaa cgcaggtacc tgtgataaaa gatcgtaaag tcatcttgcc aatcgacagt
     3781 acaggagcta tttcttattt gaagcaacaa tacttgccat tagtagaaga gttgttcgtc
     3841 agttacggat tcccaaaatt tcgtatcgaa ccagaagtgg atgagcagca agcggaacga
     3901 gtattgaaac tttttgaaga acgcaaacaa gaacaggcag aagcctttat gaagcaagca
     3961 gctgaaagtc tagttgtcca cgaacaaaag aaaaaggaaa gaaaagagca atcgccagct
     4021 cttgaaggac ccatccacat ccagctagga cgaaatattc cagccgatga accaacgaca
     4081 ccaatgatca gtatcgttga agaagaacga agagtcacac ttgaggggta cgtattcgac
     4141 aaagaagtca gagaactacg ttcaaaacgc aagatcttga ctttgaaaat cacagattat
     4201 acttcatcat ttatcgtcaa aaaattctcg aacggcgaaa aagatgaaca agtatttgat
     4261 gcgatcagtg tcggtagctg gttaaaggta cgaggaagta ttcaagaaga tacgtttgtc
     4321 cgtgatttgg tcatgaatgc gcaagatatc atagaagtca aacatacacc tcgcaaagat
     4381 tatgctcctg aaggggaaaa acgagtagag cttcatgttc atagcaatat gagtacgatg
     4441 gatgcaacaa acagcatctc tgatcttgtg gcacaagcag ggaaatgggg acaccgagct
     4501 atcgccatta cagaccacgg tggtgcgcaa gcctttccgg aagcacatag tgctggaaaa
     4561 aaagcaggtg tgaaaatctt atacggcgtg gaagcgaatg tcgtagatga tggtgtccca
     4621 attgcttata atgatgcaca tgaagcattg agtgaagcca catatgtcgt atttgacgtg
     4681 gaaacgacag gattgtctgc agtatatgac acgatcatcg agctagccgc agtcaaaatg
     4741 tataaaggaa atgtgatcga aagctttgat gaatttatcg atccaggaca tcctttgtca
     4801 cgaacaacgg tagatctgac tggtatcacg gatggaatgg ttcgaggatc aaagtcggaa
     4861 gaagaagtat tgcggatgtt ccttgaattt tcaaaagata caattttggt agcccacaac
     4921 gcggcttttg atatggggtt cttgaatact agctacgcaa ggtatggtat tcctgaagcg
     4981 gccaatccag tcattgatac gttggaatta gctagatatc tgtatcctca attcaaacgt
     5041 ttcggattag gtgtgttatc taaaaaattt ggtgtcagct tggaacagca tcaccgagca
     5101 atctatgatg ctgaagcaac tggtcactta gcatggatct ttgtgaaaga ggcaatggac
     5161 aatcataaca tgctttatca tgatcagcta aatgagcata tcggagaagg agattcttac
     5221 aaaagggctc gacctttcca tgtaacgatt ttagcaaaaa atcaggcagg actgaaagat
     5281 ttgttcaaac tgatttcaat gtcgaatgtc gaatacttcg aacgcgtccc acgaatacca
     5341 cgctcccaat tgaaaaagat gcgtgaaaat ctattgatcg gctcagcttg tgataaggga
     5401 gaaatattcg aagccatgat gcaaaaaggg gtagaagagg caagaaaccg agcaaaattc
     5461 tacgattata ttgaagttat gccaaaagcg gtctatgcgc ctttgatcga gcaagaattg
     5521 gtaaaaaatg aacatgattt ggaagaaatt atccaaaatt tggtagaaat cggaaaaagt
     5581 ttggataaaa tcgttgttgc aacaggtaat gtacattatc tgaacgaaga agatgccatt
     5641 taccgaaaaa ttttgatcaa ttctatgggc ggagcgaatc cgttgaatcg tcatagtttg
     5701 ccggatgtcc atttccgaac aaccgatgag atgctaactg catttcactt tttaggggaa
     5761 gaaacagcga aagagatcgt agtagaaaat acgaataaaa tcgcagatat ctgcgaagaa
     5821 gtgattccag tcaaagatga actttataca ccgaagattc caggttcaga agatgaaata
     5881 tcagaattga gttacacaaa agcaaaacaa atgtacggag atccgttacc tgagatcatt
     5941 caaaaacgat taaaaaaaga gttgaattct attaatggta atggcttctc ggttatttat
     6001 ctgatcgccc aaaaactagt gcataaaagt aatgaagatg gctatctagt aggttcacgt
     6061 ggctctgttg gctctagctt tgttgcgacg atgacaggaa tcactgaagt taatccgtta
     6121 gcccctcatt attattgtcc agagtgccaa tattccgagt tttttgaaga tggtacgtat
     6181 ggttctggat ttgacatgcc agaaaaacaa tgtccaaaat gtggtgcacg cttaaacaaa
     6241 gacggtcatg atattccatt tgagacattt ttaggattcc atggagacaa agtgcccgat
     6301 attgatttga acttctcagg tgattatcaa gcagaagccc ataactatac gaaagtattg
     6361 tttggcgaag actatgttta ccgtgccgga acgattggta cagtcgccga caaaacagca
     6421 tatggctatg taaaaggata cgaaagggac aataatctgc agtttcgaag tgcagaagtc
     6481 gacagattag caaaaggcgc tactggagtc aaacggacaa ccggccagca tccagggggg
     6541 atcatcgtta ttcccgatta tatggatgtc tatgatttta cgccgatcca atatccagca
     6601 gacgatcaaa attcggaatg gaaaacgact cattttgatt ttcactcgat tcacgataat
     6661 gtattgaaat tagatattct agggcatgat gacccaactg ttattcggat gcttcaagat
     6721 ttatcaggga tcgatccaca aacgatccct acggatgacc cggaagtgat gcggatattt
     6781 gccggtcctg aagtacttgg agtaagtcag gaacaaattt attccaaaac agggacatta
     6841 ggtattccag aattcggcac tcgttttgtt cgagggatgc tagaagaaac gcatcctaca
     6901 acttttgcag aactactgca aatttccgga ctatctcatg gtacagacgt atggttagga
     6961 aatgcggaag aattgattcg acgaggagat gcgacactgg cggaagtaat cggctgtcgt
     7021 gatgatatta tggtttattt gatccatgct ggattagata gcgggatggc atttaaaatc
     7081 atggaaactg ttcgtaaagg acaatggaac aagatccctg atgaattgcg agaaacctat
     7141 ctatcggcga tgaaagaaaa taatgtgccc gactggtata tcgattcttg ttcaaaaatc
     7201 aaatatatgt ttccgaaagc ccatgctgca gcttacgtat tgatggctct tcgtgtcgct
     7261 tatttcaagg tttatttccc tattttgtat tactgtgcat acttttctgt acgtgcagac
     7321 gactttgatc tagtctccat gtgcaaagga aaagatgctg tgaaacaagc aatgaaagaa
     7381 atcacggaca aaggattaga tgcttccgta aaagaaaaga atcaactgac agtgctggag
     7441 ttagccaatg aaatgctgga acgaggattc aaatttggaa tgattgattt gtataaatcg
     7501 gatgccgtta actttgttat tgaaggtgat acgttgatcg caccatttag agctgtccca
     7561 agtttgggaa ccaacgtggc aaaacaaatc gttgaagcaa gaaaagacgg tccattttta
     7621 tccaaagaag atcttgccac ccgcggtaaa gtttcaaaaa cattgatcga atatatgaac
     7681 gacaatggcg tattgaaaga tctaccagat gaaaatcaat tatcgttatt cgatatgcta
     7741 taagcatctg ttttcctgtc tgcttgtaat tttaagcaga gtatggtata gttcttatag
     7801 gtaatgatac taactgtgag tgagcggatt tttcgctcac tctttttaat ggaattacgc
     7861 acttaatttt ttaaggaggc gaaacttttg agtagcgtcg ttgaaacagt cacagaaatg
     7921 gtgacaccga ttttagatga acaaaatttt gaacttgtcg aagtcgaatt tgtaaaagaa
     7981 ggaaaaagct ggttcttacg agtatttatt gacaaagaag gcggtatcga tatcgaagaa
     8041 tgcgcctttg tcagtgagaa actgagtgaa aaacttgatg cagcagatcc agatccgatt
     8101 cctcaagctt atttcctgga ggtatcctcc ccgggagcag agcggccatt gaaaaaagaa
     8161 agcgactatg aaaaggcact tggtgattat gtccatgtct ccttatacca accagtagat
     8221 ggcgaaaaac aatatgaagg atttcttcaa tcatttgatg ctgaacagct tacattgaaa
     8281 atacgcataa aaacacgaga aaaagaattg acttttgacc gaaaaaatat tgcaaaagct
     8341 cgcttagcta tccaatttta atcacggaga ggaatcagaa aaaatgagta aagaaatgtt
     8401 aaatgcgtta gacgctttag aagctgaaaa agggatctca aaagaaatcg tgatcgatgc
     8461 tttagaagca gcattggttt ctgcatacaa acgtcactat ggccaagcac aaaacgtcga
     8521 agtggaattt gatcagaaaa aaggaaagat ccatgtctat gcggtaaaag aagtaacgga
     8581 agaagtgatg gattcacagt tggaagtctc attgaaagat gcacttttaa tcaatccagc
     8641 atatgagatc ggtgatacga tccgttttga agtaacacca aaagattttg ggcggatcgc
     8701 tgcgcaaacg gctaaacaag ttattttaca gcgtgtacgt gaagcagaaa gaacgattat
     8761 ttataacgaa ttcagtgctt atgaaaaaga catcatgcaa ggaatcgtcg aacgccagga
     8821 taaacgttat atttatgtca atcttggtaa aattgaggca gttctttcta aacaagatca
     8881 aatgccaaat gaattttatc aaccacatga tcgaatcaaa gtttatgttt cccgagtaga
     8941 aaatacgtct aaaggtccgc aagtatttgt tagccgcagc catccagacc tattgcgccg
     9001 cttatttgag caagaagtac cagaagtcta tgatggaatc gttgaaattg tcagcatcgc
     9061 ccgtgaagct ggcgatcgtt caaaagtggc agttcgctca aatgatccaa atatcgatgc
     9121 agtcggtaca tgtgtcgggc caaagggaca acgagttcaa gcaatcgtca atgaattaaa
     9181 aggcgaaaac atggatatcg tcgaatggaa tgaagatcca gctgtattca ttgcaaatgc
     9241 attgaatcct gcacaagtca tggatgtgat cttcgatgag acaaatccaa aggcctgcac
     9301 agttgtcgtt ccagactacc aattatcgct agcaatcggt aaacgcggac aaaacgcacg
     9361 tttagctgct aaattgacta accataaaat cgatatcaaa tcagaatctg acatgacgga
     9421 attttatgaa aatcaagaac aacaaaaaga aacagaagaa ctgcatgatg aagcaatcgt
     9481 tcagtcagat ttaacagacg atgaatatga aacaattgca ttcaacaatg aaacggtcga
     9541 agaaaaacca gaagcttaac tttagtaagg aggcaaagag atgaaacaaa ggaaaattcc
     9601 tttacgcaaa tccgtggttt caggtgaaat gaaacctaaa aaagagatgg tccgaatcac
     9661 ccgttcaaaa gaaggggaag tatcgatcga tccaactgga aaaatgcctg gacgaggagc
     9721 gtatgtctca cttgagccgg aagaagtaca acaagcttgg gataaaaaaa ttctcgatcg
     9781 agtactcgaa acaacattaa cagatgagtt ttatcaagaa cttcttgact atgtcactca
     9841 ccaaaaagca cgtaaagagt tatttggaaa atgaatacag ttaatcggca aaaagccatg
     9901 aatctcatcg gtctagcgat gcgcgcggga aaactaatca cgggtgaaga actgaccatt
     9961 gctgaaatcc gcaaacaaaa agcaaaattg gtgtttgtgg caaccgatgc aagcgagaat
    10021 acaagaaaaa agataaaaga taagagttca tattatgaag ttccttgctt tgagctattt
    10081 tctgaagcag aaatcacgca gatgatcgga aaacctcgca aggtattcgg aataatggat
    10141 gccggatttg cgaagaagac caaggagcta attgaaggtt aggaaggtga ttgcatgggc
    10201 aacaaaagaa tctatgaatt agcaaaagaa ctcaaaaaat ctagtaaaga cgtcgtggaa
    10261 aaagcacaac aacttggcat cgatgtgaaa aaccatatgg gtgcgattac tagcaatgat
    10321 gagaagaaat tgcaacaagc gttcaaaaac ccaagcacga aacaacaagc tgcacaacaa
    10381 gcaagcagaa aaccagccaa tcaacagccg aacgatcaac gtaaaaaaga aacaacgaac
    10441 aatcaacaaa aaaataaaaa taaccgcagt tatcaagatc gcggtcaagg gagcggtcaa
    10501 gtgaatcaag gaaaaaaacc aacaacaaac caaaataata cgggaaatac cagcagccaa
    10561 aatcgtcaag gaacgagcca aggcggcaat caaaaccgta ctaacaataa caacaaacca
    10621 aacaataaca atcgcggcaa atttaataat aataaccgta atcgttataa taaaaaaggt
    10681 aaaaaaggca aacagcaaca atcaactaaa ccagctgtac caccacgcaa attccgtgaa
    10741 ttgccagatg tattagagta tactgaaggt atgaacgtag cagatattgc gaaaaaaatt
    10801 catcgtgagc cagcagaaat cattaaaaaa ttatttatgc ttggtgtcat ggtcaatcaa
    10861 aaccaagcac tggataaaga tacgattgaa ctgttagcga cggactatgg catggaacca
    10921 caagaaaaag tccaagttga cattgctgat atcgataaat tcttcgaggc agaagaagtc
    10981 gatccagata aattagtctc acgtccgcca gtcgtaacga ttatgggaca cgtagaccat
    11041 gggaaaacaa ctttattgga tacattacgc cattcacgtg taacaagcgg cgaagccggc
    11101 ggaatcacac agcatatcgg tgcctaccag atcgatattg atggtaaacc aatcactttc
    11161 ttggatacac caggacatgc ggcctttaca agtatgcgtg cacgtggagc aagtatcaca
    11221 gatatcacga tcttagttgt tgctgcagat gatggcgtca tgccacaaac agttgaagca
    11281 atcaaccatg ccaaagctgc aggcgttcca atcatcgtcg ctgtaaataa aattgataaa
    11341 ccaggtgcaa accctcaaca tgttatgcaa gaattaagtg agtatgagtt aattcctgaa
    11401 gcatggggtg gagaaacgat ctttgttgag atctcagcga aattcggcca aaacatcgaa
    11461 gaattgctgg aaatgatcct gttagtcgca gaagtagaag atttaaaagc agatccaaca
    11521 caacgcgcaa tcggaacagt gatcgaagca cgtcttgata aaggtaaagg tcctgtgtca
    11581 accttacttg tccaagaagg aacacttcat gttggtgacc caatcgttgt cggtaatact
    11641 tacggacgtg tccgtgtaat gacaaacgat cttggccgtc gcgataaaga agcaggacct
    11701 gcaacgccag tagaaatcac tggcttgaat gatgtacctc aagcaggtga ccgtttcgta
    11761 gtcttcgaag acgaaaagac agcacgtgca gcgggtgaag aacgcgcgaa acgtgcatta
    11821 ttagaaaacc gtgctgccac aagccgtgtg actttggata acttatttga aagcctgaaa
    11881 gaaggcgaat tgaaagaagt taacgtaatc atcaaagcag atgtacaagg ttcagcagaa
    11941 gcactggctg catcattgaa gaagattgat gtggaaggtg tccgtgtaaa aatcgtccat
    12001 tcagctgtcg gagcaatcaa tgaaagtgat gtaaccttag cagcagccag caacgctatt
    12061 atcatcggtt tcaacgttcg tcctacaccg caggcaaaag tgcaagctga tactgaagaa
    12121 gtcgatattc gtcttcaccg tatcatctat aaagccatcg aagaaatcga aactgcgatg
    12181 aaagggatgc tagaccctga atttgaagaa aaaatcaccg gtcaaatgat cgttcgtgaa
    12241 accttcaaag tttctaaagt tggtacgatt gcaggtgcct atgtaacaga aggatatatt
    12301 cgtagagata gcggtgtccg tgtaatccgt gacggaatcg taatctatga aggccaactt
    12361 gcaagtctga aacgattcaa agatgacgtg aaagaagtca aaatgggtta tgaatgtggt
    12421 gcaatgattg aaaaattcaa tgacatcaaa gtagatgacg taattgaagg atttatcatg
    12481 gaagaagtca aaaatgacta aaaactgata aaaggaggcc aatcattatg gctaactacc
    12541 gtgaccgccg agtcggtcaa gaaatcttaa aagaagtcaa tgatattttg cgcaaaaagg
    12601 tccgtgaccc tcgtgtggag aacgtaacga tcacagatgt gaaagttacg ggggacttac
    12661 agcaagcaac gatctattac agcattttat ctgacttagc atcagataag aaaacagcac
    12721 aagatggatt gaataaagca tcaggattga ttcgtcgaga gttagggcac aatctaagta
    12781 tttataagac acctgaattg ttctttgaac ttgatgaatc agttgtctat ggcaaccata
    12841 tcgatgaatt gcttcgcgac ttgaacaaag actaatcaaa aactcccgca aatcctgtga
    12901 aatcaggggt tgcgggattt ttttgttttc attaagacgt attacctaag agttccatga
    12961 ctttagtctt gcggcgtgtc agctaaccaa aaaagagaaa aagaaaagta aaaaattcga
    13021 ctttcttttt tctagtagta tataaatttt tgcatataga taaagatcaa gataccgata
    13081 acggaagcta gattgtaatc tattttagtg acagtcaggt atagccaagt aactagaaga
    13141 tcagtggaac ctgcaccata aaagtttgag tttgtcggtc cgttgacagt taaaagaaaa
    13201 atcacgttga agttattgat atttccgatt acgccgcttc gatttggtca cgatccaaat
    13261 tttggataat ccctgtagaa attagcatag tagctgggat accaattcac atattgacaa
    13321 agataacaga gacttttaca taaaacggat caatcaggaa aggaattgct cgatccagcc
    13381 ccagttttcc agcaatgtgt taactggccc agcgtcattc agcaaattat gcatgatgag
    13441 gagcgaaatg aactgcggaa cagccattgt gataacgaca agttttttgg atcaatgtaa
    13501 aaaacgtcag cgaaaggaaa aatttcgtgg ttttttatac ctgttcattt ggctagatat
    13561 aaaatccaaa caaatgaaca gaagtgctta ttaggtttca attgggagta tctgcgctag
    13621 aataagagta ataatgaaaa ggagtgttat gtatggatta ttctagtaaa gaatactttg
    13681 ataaaatgac ggcttggtgg cgtgctgcta attatctttc tgttgggcaa ctttatctta
    13741 aagataaccc cttactgaga cgtacactga aacccgaaga tgtcaaaaaa catccaatcg
    13801 gtcattgggg aacgattcct ggccaaaatt ttatttatgt ccatttgaac cgagtcatca
    13861 ataaatatga tttgaacatg ttttatattg aagggccagg acacggcgga caagtcatgg
    13921 tatcgaatgc ttatttagat ggaagttaca cagagatcta tccagaagta acggaagatg
    13981 agacaggaat gcaaaaatta ttcaaacgat tttcatttcc tggaggcatt gcttctcatg
    14041 cagcaccaga aacaccaggt tctatccatg aaggcggaga gcttggatac tctctttcac
    14101 atgcagtcgg agctgtatta gacaatcctg aagtaatctc tgctgtcgtg attggtgatg
    14161 gtgaagcaga aaccggtcct ttagctggaa gctggttctc aaatgtcttt atcaatccag
    14221 tgatagatgg agctgtttta ccgatccttc atttaaatgg tgcaaaaata gccaacccaa
    14281 cgatcttggc acgtaaatca gatggagaat tagcaaatta ctttaacgga cttggctggg
    14341 aacctttctt tattgaagga aatgattcag aaaaattaaa tcctgttatg gctgaaaaaa
    14401 tggatcaagc aatcgagaaa attaaatcca tccaaaaaga agctcgcctg aagacagcaa
    14461 cagatgtcgt gatgccaaaa tggccagtat tgatcgtacg cacaccaaaa ggatggaccg
    14521 gtccaaaaga atgggacggg gaaccaattg aaggaacctt ccgagcacac caagtaccga
    14581 ttcctgtaga tcaagaacat atggatcatg cagatgcttt actccgttgg ctgaaatcct
    14641 atgaaccaga aaaattattt gatgcacaag gacgtatttt agaagaaatc agagaaatcg
    14701 cgcctactgg tgaccaaaga atggcaaaaa atcctatcac caatggtgga atcgatccaa
    14761 gaccgcttat tatgccagat tggaaaaaat atactttgca atttgaaaaa ccaggctccg
    14821 ttaaagcaga agatatgacc gaacttggta aattcgttcg cgagatcatt gagaaaaatc
    14881 cagaaaattt ccgtatattt ggaccagatg aaacaaaatc aaaccgattg aatcaagttt
    14941 tcaaaacgac aaatcgccaa tggatggaaa aaatcgaacc agaaaatgat gaatggcttt
    15001 cgccatctgg tcgcgtcatc gacagtcaat tatcggaaca ccaagatgaa gggttcttag
    15061 aagggtacgt tctgactgga cggcatggtt ttttgcaagt tacgaatcat tcttgcgagt
    15121 ggttgactct atgttgactc agcacttcaa atggatgcgg aaatcacatg acctttcttg
    15181 gcgaaatgat tatccttcgc taaatctcat tgcaagctca acagtcttcc agcaagatca
    15241 caatggatat agccaccaag atccaggaat attgacacat ttggcagaaa agaaggcgga
    15301 gttcatccgt gaatatctgc ctgcggatgc aaatacattg ctcgcagtca tggataaagc
    15361 attccgctcg tcagaaaaaa tcaatttgat catctcttcc aaacatccgc gtgctcaatt
    15421 ttactcagca gaagaagcag ccgtcttagt gaatgaaggc ttgaaaatca ttgattgggc
    15481 ttcgacagca aaagaggagg aacctgaatt ggtcattgct gctgcaggaa cggaatctaa
    15541 cttagaagca ttagcagctg taactttgct gctggaagaa ttcccaaaac taaaaattcg
    15601 gttcatcaat gtcgtagatt tattgaaatt acgtcatcca agtcaagatc cgcgtgggtt
    15661 gagcgatgaa gaatttgatc aatattttac aaaagacaaa ccgatcttat ttgctttcca
    15721 cggttatgaa acattggtcc gcacgatttt ctttgatcgt cataaccatc atttaatgat
    15781 tcatggctac aaagaaaatg gagatatcac gacaccattt gacatgcgtg tagtaaatga
    15841 acttgatcgt tatcatttgg caaaagacgc agctttaaaa atcaaaggaa gtcaagcaga
    15901 agattttgcg aaaaaaatgg atcagaaatt acaagaacat caaaactata ttcgtgaaaa
    15961 tggtattgat ctgccagaag tattggattg gaaatggaaa aatcttgacc aataaaatga
    16021 taaaatcgat cccgcaaaat agaatttttt acattgatta agaaactgaa aaaataaata
    16081 aaaaaaacgg aaaaaagact ggaagaatgg ccagtctttt ttttcgtttt tgggaagtga
    16141 aaaccaaact atttacctgt aagccaccca taaatccaat caaatggtgc aaagaaaaaa
    16201 tcttccattc tattccctcc tttaaaaaag cataacatga tttctattta cgtgagcatg
    16261 aattttgtta cgtttttatt gccgaaaaat aaaaaccgtt cattatgatg tcaaattcgt
    16321 cagtaattga tgcaatgtgt tataataatt cagttaaatc aaaaatgaaa tggagaaaag
    16381 acatggacgg gttattacct ttatggaaag aacgcggaat gacaagtcat gattgcgtat
    16441 tcaaattaag aaaaatctta catacgaaaa aaatcggaca tgggggtacg cttgacccag
    16501 acgtagacgg tgtgttgccc atttgtattg gaaaagccac aaaagttatc gaatatttga
    16561 cagattctgg gaaaatctat gtaggcgaga taactctagg tttcagtaca acgacggaag
    16621 atcgatccgg agaacgggta gaaacaaaac cagtagatat gtctttatct gaaaaaaaaa
    16681 tagatgaagt gatggcttca tttattggtg agatcaccca gataccacca atgtactcag
    16741 cggtaaaagt caacggtaag cggttatatg aatatgcccg caataatgaa acagtggagc
    16801 gtcctgttag aaaggctcag atttaccgtt ttatccggac aagtgaattg acaagagacg
    16861 aagtgacagg aaccatttct tggcgcttcg aagtagaatg cgggaaagga acctatgtcc
    16921 gcacattagc tgtagataca ggcaagaaac ttggttaccc tgcgcacatg tctgatctga
    16981 cccgaacagc aagtgccggt atgtcccaga aggatgctct gacattagaa gaagtagctc
    17041 aagctgtgaa aaatgggaca atcgatgatt gtttgctgcc aatagaaaaa ggagtggaac
    17101 ggttcaaacg agtagatatt actgatgaag tatggcagaa agtaaaaaac ggtatgagac
    17161 ttgattatac tgtatttgcg ctcccagaaa tgccgtcaga agaaattgct ttgttttata
    17221 aaggaaaagt tgtcagtatt tatcagccaa acccgaaaga aaaaaacaaa ttaaaaccta
    17281 gtaaagtatt gagaaatgag gaataacgaa tgaatatcat caagatcaga catccttacc
    17341 aatcagaaca aattccagaa gacgatgtcg ttttagtgct gggatttttc gacggcgtgc
    17401 atatcggaca ccagaaagtc ataaagacag gtatggaaat tgcacaaaaa gaaggattga
    17461 aattagcatt gatgactttc aatcagcatc catctattgt ctttaaaaag atcgatccta
    17521 gcagcgtgaa atatttgact agccttaagc aaaaagaaga aaaaatggcc gctttgggta
    17581 tagattattt atatgaagtt gattttactt cttcttttgc tcatttagca ccgcaggaat
    17641 ttgttgatca atacatcgta gggttacatg caaaatatgc tgtttccggt tttgattata
    17701 cgtatggacc aaaagacatt gcagatgtcg ctcattttcc tgaatatgcc aaaggaagat
    17761 tcaaaatcgt cacagtttct aaagaagaag aacaaggtga aaaaatcagc tctacccgta
    17821 ttcgtggatt actagacagc ggacaagtaa aagaagcagc aaaactatta ggttgcacat
    17881 atgaggtaga aggggtagtt gtccatggcg atgcacgagg cagactacta ggattcccta
    17941 cagcaaatgt caaagtaaag agcacggttc atttgcctaa agaaggtgtt tatgtgtcgg
    18001 aaataaaaat tggggaaaca tggtatccag ccatgggttc aattggacat aatgatacat
    18061 tcggtgaagg acgggagctg actgtagagt tgtatatttt ggactttaat caagatatct
    18121 atggtgaaca tgtcgcggta cgctggaatc aatttttaag agatcaagtg aaattttcta
    18181 gtgtagagga attgattgaa caactcaagg aggatgaaca agcaacagct gaatattttt
    18241 ctcagcaata aaacattatg cataaaatct cttgtttatg tataactagc aattacaata
    18301 aaatgggtga taaataagcc caggaaaggg gctgtgacaa aagtcttgtc acagcctttt
    18361 tttccaaata aatggttttt cagaagtagc ttcttcggaa ataagccgaa atttcttgaa
    18421 gctgaccact tctgtcacaa cctctttctt tatagaaata aaaggacact agcaattcat
    18481 gaaaacgatt gctagagcgt atcatttgct ttagactata tatcttggtg tacactcact
    18541 ttaaggcgga aataaaagtt atccgtaata acaggtttag gtatgaaaaa ggagtgggta
    18601 ctatgtacaa ggaattagaa aacaaagtcg cagtagtaac tggtggctca aaggggattg
    18661 gaacagcgat tgctcaacga ttcgggaaag aaaaaatgaa tgtagttgtc aactataact
    18721 cggataaaga aggagcagaa aaagcagtca aagcaatcga agaggcaggc ggaaaagcag
    18781 tctctgtaca agcagatgtc agttctgaag aaggaataca gcgtttactt gatacggctt
    18841 tagaaaattt tgggacactg gacgtttggg ttaataatgc tgggatggaa aatcagcatc
    18901 caacccatga acttagcctt gaagattggg aaagagtgtt aaaagtcaat ttgactggcg
    18961 tttttatcgg aacgaaagcg gctttaaatt actttacaga gaataataaa aaaggaaata
    19021 tcatcaatat gtcttctgtt catgaacaga tcccatggcc aacgtttgct cattacgctg
    19081 cttcaaaagg cggggtaaaa ttatttaccg aaacggtagc aatggagtat gcaccgaagg
    19141 gaatcagaat caatagtata ggccctggtg ctatccgtac acctattaat gcggcaaaat
    19201 ttgatgatcc tgaacaaaaa gaactcttgg aaagtatgat cccactagga cgcgtaggtg
    19261 aaccaaaaga aatagcggcg acagctgcat ggttggcttc agacgaatcc agttatgtta
    19321 caggtattac actatttgca gatggtggga tgactttata tccttctttc caaggtggaa
    19381 aaggataagg gtgaataaaa atgggtttat tgattgcatt gatcccggca gttggctggg
    19441 ggatccagcc actgattttg aaaaagattg gtggcagacc aacaaatgag atattaggaa
    19501 ctggtattgg agcggtgctt gtcggcttac tcgttcaatt attcgcttct ccagctggta
    19561 tttcagtgac tacatttttg attagtttac tttctggtgc attttgggtt attggacaaa
    19621 caggacagta tacatctttc aacttgattg gtgtttctaa aacgatgcca atttcgactg
    19681 cattacagtt agtcggaacc tcgttgattg gtgtcttcgc ttttggagaa tggtctggaa
    19741 gtatggggaa aatcattgga gcagcagcaa tcattcttct tgtatttggt tctgctttaa
    19801 cggcaatcag tgatgatcct ggaaaaaaag gcggtatcac aaaaggaatt agtctattag
    19861 catcaacaag tattggttat tgggtataca gtgcattgcc taaactagtt gatgcaagcg
    19921 gactttccat tttctttccg caaatgatcg gtgtcttttt aggggcagtg atttatgtga
    19981 ttttccgtaa accaaccgca ttttctgaag acaagagttg gaaagcaacg atcgttggag
    20041 ccatctttag tgtgtcagca ttggcttata ttttttcagc aaatgaaaat ggggttgcta
    20101 ctgcatatat cattacacag ctaaacgtag ttatctctac attaggcggt atgtacatcc
    20161 tgcatgaaaa taagacacca aaagaaaaaa aattaacgat tatcggatta ttgctgatcg
    20221 tagtaggaag catggtcacg gtctttattt gaggataaag atggtaaaat ttcatttatt
    20281 aaaaaaatat ttgtttgaac caaacagtaa gtacttataa aaaaatagcg ctagaatttc
    20341 caacatctct ggaatttcta gcgcttttta attgtttaag atacaaggga aatatctgtc
    20401 tggatcaata atttttcatt gatccattga ctaaagttta cgacataatt gaagttacca
    20461 ccgattagta tctccgcatg attgtcttta taaagatcgt attgtaaaag acggtctttt
    20521 ttatttgcgt aaacatcgtt atggtactgg actaattgtt ctcggaattg attgattgct
    20581 gtaaaatcac gatattgaag gttgaaacgc atcgtgattg gtgcatttag catgaatcgc
    20641 tgcatttctt ttgctgcttc ctctgcttga tctccgtata ggaactcaaa aagattacgt
    20701 tcagaaaatt tccattgccc gccaaccttt gttcctttta gttttccttc atgaagataa
    20761 tttctgatag aacgtgtagt aacacctaat tgttcggcta attcatttac tgagtaaaaa
    20821 tctttcaatg ttcaccctcc tatgtgaatg gttctactga cttagttgat atttgcttaa
    20881 tcacgaaatg tagaaagaac gattttaatt aaggtcattg taaaaatcaa gtcagaaaat
    20941 tctaactttt atttctctaa agaaattcgt tctctcctaa agtgatatta tatttaaata
    21001 tacctgaaaa taatgggcag tattatgaaa gatcactgat attttctcgt attttttcct
    21061 tattcaaaag ttttgcagga aaattgctga aaaaataatt ttactatgta gaaaattcaa
    21121 agccattcaa tcagtgattt ttttcagcac aagaaagact ccttataaaa atagcagata
    21181 ctttgtcaat taagtgttta accagggatg ataaaaagga taggattgaa caaaacctgt
    21241 ataaggtgat gaatagtttc ctcgccagta aaaggtttat tcgtataaag ctttatcgcc
    21301 aaaatttatc atgacctttt ttatcttcaa tattaaatga aatcaattgt tcaatcaatt
    21361 cccagttgat tacacttttc caaggaactt tgatgacgtt atccgtatat acgtagcctg
    21421 cttcttggat atttttttca agggcacgaa tcgctgcttt ttccggtgcg atagaaaaat
    21481 gatttttagc aaaactaaat gcaataataa aggtaccatg gtcagtaaac ataggttggt
    21541 tccattttac agttgtgtct agctggggat acgtttctgt tatcttagca aaaaggtctt
    21601 tgatcttttg ttgatgttct ttttctttga tttgattgat gatcggctga aatttttcca
    21661 ttctaaaacg cctccattaa gtggtttgat ttattttacc agaaaaacaa tagttatgta
    21721 taaaaaacga acaaaacaca agtaatgttg taaccgctat atttatatga taagatgtct
    21781 caaataataa aatagaaaag agacataaaa atgattgact tggcaaagaa attcttacac
    21841 gaaaatatat attttgagtt gtatcatcat cgagccattt atacaaatga agatgctttg
    21901 gtaataaaga aagaacaagg atttacaggt acagagacca aaagcttatt cttaaaagat
    21961 aaaaaagata ataactacgt atatctgact tttactacga aacaaacgga tttcaaagca
    22021 ttagggaaga tcgtcggtaa aaggctttct attgtttcat cagaacgcat ggaagaacaa
    22081 acgggacaaa agcagggcgc tgtttctccg tttggttatg aaaaacagat tccgattatt
    22141 attgatgaag agctcttatc aaatgaaaaa cttgtttttg caccaggtag accagatcaa
    22201 acgatggtca tccaaacggt tgacttaaac aaaatcatcc agctacttga gatgccgata
    22261 tttttgatgc cggcaaacga aaaaaacaat taaccaagta aaatagtgca tacttggagg
    22321 tgaagcaaca tgataagtta tccattatta gaaagaaatg caacgattgg ggttactgca
    22381 ccatcctcag gcgtatcaga ggaacaccat tcgttactcg aatcagcttt aaagagaatg
    22441 gaagaaaaat ctttccaagt agttactgga aagacgcttt ggacgcaaga taaggaaaaa
    22501 tcaggtccag caagaaaacg agcagatgaa ttcaatcaaa tgatgcaaga tgaaaatatc
    22561 caaatgatct ttccgacttg gggaggagaa ttattgattg agattctcga ttttgtcgat
    22621 tttgatgctc tacaaccaaa atggatagta ggttattcag atattagtac gttgctactt
    22681 gctgtcacat taaaaacagg gatagccact gcacatggaa ctaaccttat tgatttaaga
    22741 ggagagtact tggatgaaac gacagcaatg tgggaaagtg tactgaaaac aagagaagga
    22801 gaatcgattc tacagcaatc ttctgaaaag tatcaaaaag tgtggcccca tgagcattca
    22861 tcatcttctg ttttccatct cactgaacca acaaattggc aatcagtcag cggagatgca
    22921 gtaaggatga agggacgatt attaggtggt tgtattgata cgatcaggca tttaatcggt
    22981 actccttatg gagatgtcca atcttttaaa agaaagtata atgatggaaa gccgatcttg
    23041 tggtttctag aaaactgtga gctatcgtca gttgatttaa gaagatcact tgttcagatg
    23101 aaactggcag gctggtttga tggcagctcg gggatcatgt tcggtagaag tgcggcagag
    23161 gatttgttag attataaaat gaaggatgta tacgaagatc tgtcaaagga attaaaacta
    23221 ccgatcttgt atgatattga ttgtggtcat gtaccgccac aactgacatt gattaatggt
    23281 gcctatgcag aagtagcggt agaagataga aaagcgactg ttttgcaaac gtttatttaa
    23341 aaaatttttt cctgccttgg agtctatcaa agaccaataa atctaaatag aaaagaaagt
    23401 gaaagaagct gacaaaatgc tgtcagcttc tttttgtata acatattaga aagtctaatt
    23461 atctataact gtcacatcga ttaactgttt cacgtcattg ggatttcctg taggaatagc
    23521 tactacagat gcattatcaa tgatttcttg agttgtttct ctaatgaacg ctggataaga
    23581 tgtttgatct gcaggcattt gccaatgtga aggaactgga taactagtga ttgtcccgtc
    23641 atggttactt gaatacacta aaactccctg gtaactgtat ttcccagtga gcataaccac
    23701 atctttcgga tagactgcac tccctgcatc ataaggggcg ataggtgttc ctgcaggata
    23761 atgaataaca tgtaattcga aatcacccga tgtatctcta aaagtattac ctctaatagc
    23821 tagccaaaca cgtgcgtact caatttgtag atcagaatat cctgctaacg gatctattga
    23881 ctgagaagac tgtgaggagg ttgtgtctat tgttgaagaa gaaatatttg tctcaactgt
    23941 actactagat gtagaagaag tattgggctc tgagacttta ctgcttgttg aagatgtatt
    24001 cgtttcagta gaagctgatt tctgagtact tgatttttca gctgaactgg tttgtctgat
    24061 tgaacttttg tctgtggtcg cagatggatc actattttct ttactaggag acgtacatcc
    24121 tacaaggaat aaagagacca acaataaaat aaaacctaac tttttcatat aaaatacctc
    24181 attatttttt taataccagt atagcataat cctgaatttt tgaatccgta aaaccttgtc
    24241 tatgaatata aatagagtgt atcttctttg ataaatgtca gaaaaagtgt gacaaataat
    24301 catcagtaaa attacttgtc acactattga taaggagtat ttaattgaag agaatgatta
    24361 ggagaaagga tctccgtact ttttatagta atcatagaat ccaagtttgt atagacgaat
    24421 cgttttttct cttatttctt ctgtttcaaa atggactgct tgaagaatca agtctgtgaa
    24481 ttctatagga gctgttccat tcgcagtaat caaattacga tctttcacag cttgttgttc
    24541 tataaaatct tctttattct gataattttc cgattctaac caaggaaatt gcgcatttcc
    24601 ggtgtgtttg taaccttgca ataatccgct ctgagctaaa taatcaactg ccccacaaat
    24661 agcagctact ggtacgtctg tttttaacaa tttatcgatt atttgatata aatgatgatt
    24721 ttttatgtcc catgaattgc caccgatcat aataaaaagg tcaacttttt caggtatttg
    24781 ttcgattaag taatcgattt ttgtttttaa tcctccaatg gaagtgacct ctgattggat
    24841 agaagcagtt ttgttttgcc atttctcatt ttggtttagt gtgctagtaa tataagcacc
    24901 ttcccaatcg gcatattcct ctaatagaaa cgtaatcgca gttttcattt tcaagctctc
    24961 ctttactatc tataacaagg acaacagttt taacctacag acattcatta aaattgggta
    25021 gcgacagcga aaacaacaat tcttaagttt tagacagagt atacagatag tcaacttaat
    25081 taaatacgaa ctaaataaaa atgacaggta atcatttttg tttaccagca ttataacatt
    25141 ttgcatacat ggatgacaaa agaaccaatt cccacttgct tgagtacaat attaaaagaa
    25201 actggaaggc aaaaagtaat tatagaaacg aaaaataaat catatcgggc actcagacat
    25261 gaaaacaacc ctagagatat ataactaggt aaccagttta atgaaagaaa aaatcatcca
    25321 agaagtcgat tcttggattt tttagtggat ttgttcgcca aatacgttta gtatgcgtat
    25381 atgagaggtt gtaagcaaag cgttgtggtc tgagcactta aggaggaatc tgtgaaaata
    25441 gcttttcata tttttgcaga ttttgactta atgccgaagg tcagctttgc gattaccgtt
    25501 tatcgagagg ctgtaaaagc accgtgtagc tccgatgaca ataagcaagc atttttagag
    25561 ctaggtttcc ttgattctaa atatgcggct tatttcaatg agttggtgct tgaacgcgat
    25621 tttaagaagg tcgaaaacag gcacgacttt tcgacgtaag gagaaagaat ttcaatgata
    25681 gaaacaataa atcccgcaga aaacaaggtt tctgcgtaca ttcatatccc attttgcgag
    25741 catatctgct attactgtga ttttaataaa gtttttttag aagggcagcc agtagatgag
    25801 tatgtggaaa tgctgctgaa tgaaatgaaa ataatgctag aaagtcatcc gacagcgaat
    25861 ttagaaacac tttacgtagg gggcgggaca ccaacttctt tatctgctaa acaacttgat
    25921 cgactgttat caggtgctag agagatcctg ccttttcaaa atggtaatga gtttactgta
    25981 gaagcgaatc caggtgattt gacccgtgaa aaacttcaag taatgaaaaa ctatggcgtc
    26041 aatcgtttat ccatgggagt gcagacattt gataatcgct tattgaaaaa aattggacgt
    26101 aaacacaccg cagaagatgt gtatcaaact atgcaatttt tagaagcaga aaattttacc
    26161 aatgtaagca tcgatttgat ttatgcatta ccgggacaaa cactagaagg atatctggat
    26221 actttggaaa aagctttggc tttggatctg cctcactatt ctttgtactc attgatcctc
    26281 gaaaacaaaa caatgttcat gaactgggta cgacaaggaa gactcgaact gcctgatcag
    26341 gaaacggaaa ccagaatgtt tgaagagacg attgaagcaa tggaaaagca tggacgtcac
    26401 caatatgaaa taagtaactt tgggctctcc ggacatgaat ctcaacataa cctcatgtac
    26461 tggaacaatg accactattt tgggtttgga gctggtgcta gcggttatct tggagatatt
    26521 cgttaccgca acaaaggacc aatccaacac tatttacgcc cattacgagc aaatgaacgc
    26581 ccggtattgg aagaagaaaa attgacacga aaaaatcaaa tagaagaaga aatgttctta
    26641 ggactaagaa aaaaaacagg gattactaaa aaacatttct atgatcgcta tcaaaaaaca
    26701 attgaatcgg tgtacggtga tgtgctaagg gaattagagc aagaagggtt acttgttaat
    26761 gaagaagacc gtgtttattt gacaccaaga gggacctttt tggggaacaa tgtgtttgag
    26821 cgctttttgt ttgaaaaata gtttaaagaa tatttgtttt ttatctgaat ttaaataaaa
    26881 aaagttcaaa aatacaataa aggactattg aaacgttgat ataactggat ttacatcaat
    26941 tatttagaat tagcactctt gtgtttagag tgctaaaaaa tggagtcttt ctcttgacag
    27001 gagaagggct tcttgctata tttataagtg gattagcact tagaatatat gagtgctaat
    27061 gagaggtgag gaagatgtta acaaagcgac aaaacgacat tttacgtctg atcatccaac
    27121 actatacgag tagtggcgtg cccgttggtt ccaaaacatt gatgtctgag ggagtcaagg
    27181 ccagctcagc aacgattcgc aatgatatga aagcattaga agatgaagga ttgctgttga
    27241 agactcactc ttcttctggg cggattccat ctgctttagg ctatcgttat tacgtggatc
    27301 atttgttaaa acctgctcgc gtagcagaag atgatttgct gctgattcgt cagtctctag
    27361 gcagagaatt tcatgaaatc aatgagatta ttaagcaatc tgctgagata ttatctgagc
    27421 tgacaagcta tacgactttt tcattgggac ctgaaataaa agaccgccga ctgactggct
    27481 ttcgtatcgt acccttgaat aatcgacaga ttattgctat cgtcgtaaca gatatgggga
    27541 atgtagaaag ccaagtgttc actcttcccg aatatatggg aagtcaggaa ctagaaaaaa
    27601 tggtacagat tgtaaacgac cgtctagtcg gagatcctct gatgacggtg taccataaac
    27661 ttcgtacgga gattccgatg attcttcata aatatttcca aacgacagaa gggatgtctc
    27721 agttatttga caccgttctt ggtcatgttt ttgaagataa ggtttatgtc agtggacaaa
    27781 tgaatctctt ggattatgag ccccgtcaag atcttgatca gttcaaatcg atgttttctt
    27841 ttatgaaaga ctcagacgga cttacacaaa tgatcactcc gatggatagc aatattcata
    27901 tcaagattgg tgctgaatta ggcaatgatt tattgcagaa tatgagtatg attcaagcaa
    27961 gttacgagat agatggtcat ggaagaggta caatagcctt gttagggcct actagtatgc
    28021 cttattctcg gatgttcggt ctagtcgatg tttatcgaag agaactagca gctaaacttg
    28081 ctgattacta ccgttcgctt gacttgtcaa actcgtaatg tgaataacga agctatttgc
    28141 agctgatcgt tctctctttc ttagtaagag caatcaaaca gctgactgaa taatgaaagg
    28201 aagactgtat cgtgaaagaa aatcaagaag aactagataa agaaatgaca gatgctcagc
    28261 cagaaccgga aatcgatgtc gaagctgcag aagattcggg tatttcagaa gctgaagcgg
    28321 aagaattcga aacagcgaaa ttaaaagctg agttagaaga aatggaagat aaatacttgc
    28381 gggctcgtgc tgaaatcgca aatatggcaa atcgaggaaa aaatgagcgt gagcaattac
    28441 aaaaataccg ttcacaagat ttagccaaaa aattacttcc atcaattgac aacctggaac
    28501 gtgcattagc cacagaagtg tcagatgatc aaggggcagg tttgaaaaaa ggcgtggaaa
    28561 tggtcttgga aagtttgcgt aacgcactgg aagaagaagg aattgaaaaa atccctgcta
    28621 aaggagaagc ctttgatcct aatcttcatc aagcagtaca aactgttcca gcgactgaag
    28681 atacaccagc cgatacgatt gtagaagtac tacaagaagg ctacaaatta catgatcgcg
    28741 tgttaagacc aaccatggtc attgttgcac aataattgtg cggatataaa acttacactt
    28801 tcaattaaag aaggagacgt taattatgag taaaattatc ggtattgact taggtacgac
    28861 taactctgca gtagcagtat tggaaggaaa cgaagcaaaa atcatcgcta acccagaagg
    28921 aaaccgtaca acaccttctg ttgtatcatt caaaaatgga gaaatccaag taggggaagt
    28981 tgcaaaacgt caagctgtga caaacccaaa tacgatttca tcaatcaaac gccatatcgg
    29041 tgaagcaggt tacaaagtag atgtagaagg taaatcatat acaccacaag aaatctcagc
    29101 aatgatcctt caatatatca aaggattcgc agaagactat ttaggtgaaa aagtcgaaaa
    29161 agcagttatc actgtgccag cttactttaa tgatgcacaa cgtcaagcaa caaaagatgc
    29221 tggtaagatc gctggtctag aagtagaacg tatcgtcaat gaaccaactg cagctgctct
    29281 tgcctatggt ttagataaaa cagatcgcga cgaaaaaatc ttagtattcg accttggtgg
    29341 cggtactttt gacgtatcta tccttgaact aggagacggt gtctttgatg tattgtcaac
    29401 agctggcgac aaccatctag gtggggacga ctttgataac aaaatcatcg actacatggt
    29461 agcagaattc aaaaaagaaa acagcattga cttgtctcaa gacaaaatgg ctttacaacg
    29521 cttgaaagac gctgctgaaa aagcgaaaaa agatttatct ggcgtatcaa gcacacaaat
    29581 cagcttgcca tttatcacag caggagaatc tggaccattg catttggaaa tgacattgac
    29641 acgtgcaaaa tttgatgaat taacagctga cttagtagaa cgtacaaaag taccagtacg
    29701 tcaagcgcta aaagatgcag gtttatcaca atcagaaatc gacgaagtta ttttagtcgg
    29761 tggatctact cgtatccctg cagttgtaga agcagtacgt aaagaaacag gaaaagaacc
    29821 aaacaaatca gttaaccctg atgaagtagt tgctatgggt gctgctatcc aaggtggcgt
    29881 gatcactggt gatgtgaaag atgttgtttt acttgacgtt acaccattgt cattaggtat
    29941 cgaaacaatg ggtggagtgt tcactaaatt gattgaccgc aatacaacga ttccaacaag
    30001 taaatcacaa gtattctcaa ctgcggcaga taatcaacca gcagtagaca tccatgtatt
    30061 acaaggtgaa cgcccaatgg ctgctgacaa caaaactcta ggaagattcc aattgacaga
    30121 tattccagca gctcctcgtg gtattccaca aatcgaagta acattcgata ttgataaaaa
    30181 cgggattgta aatgtatctg cgaaagatct aggtacacaa aaagaacaaa aaatcacgat
    30241 caaatcttct tcaggtctaa cagacgaaga aatcgaacgt atggtaaaag atgcagaagc
    30301 aaatgcagaa gcagataaag ctcgtaaaga ggaagttgac ctacgtaatg atgtagatgc
    30361 attgctattc tctgtggaca aaacattgaa agaattagaa ggcaaagtgg acgaagaaga
    30421 agtgaaaaaa gcagaaacag ctcgcgatga attgaaagcc gctgttgaag caaacaacat
    30481 cgaagaaatg aaaactaaac gcgatgcatt gaacgaaatc gttcaaaact tgactgttaa
    30541 actatacgaa caagcggcac aacaacaagc tcaagaaaac ccagaagcag ctcaaggcgg
    30601 agcagatgat gtcgttgatg ctgattttga agaagtcgat ggcgacgata aataatcagt
    30661 tcactgaatt tcgagagaag gccaaagttg ctactttggc tttttctttt gaaaaatagt
    30721 ttgaagtatc ggtttttgtt tcaaacaaaa tatgatatga taacaagcga cgtgaaaatg
    30781 atgagaacca ataggaggaa agcctatggc aacaaaacgt gattattatg aagtcttagg
    30841 gctctcaaaa ggagcatcag aagatgaaat aaagaaagct tatcgtaagc tttcgaaaaa
    30901 ataccatcct gacatcaata aagaacctga tgcggaagaa aaattcaaag aagtatcaga
    30961 agcatatgag attttaagtg acccgcaaaa acgcgctgca tatgaccaat acgggcatgc
    31021 aggcacagat ccaaattacg gtgctggtgg agctggtggc ggatttggtg gattcggcgg
    31081 cttctctggt ggcggcttcg gtggttttga agatatcttc gactctttct ttggaggagg
    31141 cggtcggtca gtcgatccaa atgcgcctcg tcaaggtgct gatttgcaat atacggtcga
    31201 cttgtctttt gaagaagcga tttttggtgt agaaaaagaa atcaagtata atcgggaaga
    31261 aatctgtcat acttgtggtg ggaacggtgc aaaaccaggc acacaaccaa ctacttgtca
    31321 taaatgtcat ggtgcaggta cgattaatgt agaacgccaa acaccacttg gacgagtgat
    31381 gagccgtcaa acttgtgatg tttgtcatgg gacagggaaa gaaatcaaag aaccttgtcc
    31441 aacctgtcat ggctcaggac atgagaagaa agcacattca gttaaagtga atgtaccagc
    31501 tggtgtagaa gaaggacaac aaatgcgttt agctggtcaa ggagaagctg gagaaaacgg
    31561 cggaccattt ggtgatttgt atgtggtctt ccgtgtagaa gaaagtgata tctttgatcg
    31621 tgaaggttct gaaatttact atgaattgcc tttaagtttt gtacaagcag ctttggggga
    31681 tgaagtccaa gtaccaactg tccatggaga tgtgaaattg aagatccctg ctggaacaca
    31741 gacaggtacg aacttccgtt tacgtggaaa aggcgctccg aagttacgtg gtggtgcaac
    31801 aggcgaccaa catgtcaaag taaaactgat cacaccgaaa aatctaaatg aacaacaaag
    31861 agaagcatta cgtgcattcg ctgaagctgg cggcatcaaa gttgaagaac agcaagaaga
    31921 cggtttcttc gataagttta aagatgcttt tggcgggaaa aagcggaagt agtttttcta
    31981 gtaaaaacgc taagtcatta ataattaaa
//
LOCUS       DACDKM010000020        29819 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20834_2016
            SAMN12640370-rid9891663.denovo.020, whole genome shotgun sequence.
ACCESSION   DACDKM010000020 DACDKM010000000
VERSION     DACDKM010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640370
            Sequence Read Archive: SRR10040891
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 29819)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29819)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742835.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742835.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:04:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,012
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 29 of 75
            Pseudo Genes (incomplete)         :: 33 of 75
            Pseudo Genes (internal stop)      :: 19 of 75
            Pseudo Genes (multiple problems)  :: 5 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29819
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640370-rid9891663.denovo.020"
                     /isolate="E20834_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(116..1060)
                     /locus_tag="IV334_000940"
     CDS             complement(116..1060)
                     /locus_tag="IV334_000940"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HAP8943030.1"
                     /translation="MKKTSRKVVIVGTGFVGTSIAYAMINQGISNELVLIDVNQEKAE
                     GEALDLLDGMAWGDENVAVWSGGYEECKDADIVVITAGINQKPGQSRLDLVKTNASIM
                     RQIVKEIMGSGFDGIIVVASNPVDILTYIAWNESGLPTSRVIGTGTTLDTTRFRKEIA
                     LKLKVDPRSVHGYILGEHGDSEVAAWSHTTVGGKPVFEIVEKDHRIAQDELDVIADKV
                     RNAAYEIIDRKKATYYGIGMSTARIVKAILNNEQAVLPVSAYLTGEYDEKDIFTGVPS
                     IVDENGVREVVELSINEEEKAMFSKSTSALREVLNTVL"
     gene            complement(1356..2300)
                     /locus_tag="IV334_000941"
     CDS             complement(1356..2300)
                     /locus_tag="IV334_000941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP8943031.1"
                     /translation="MRNEESIETLQREQGMNINDLNLAYHLQTPPKIHRGDLFQQGNI
                     HISKHRRYAEVPEHTHDFVEINYMYSGTCLQIINGERIEMEESDVVIIDKEATQRIEY
                     TGQEDILINILLKEETLSTEILNHLASTNNLLSSFLINASKEKGEHTQFIHFRGGKTS
                     RLTHLIENMLLIYFDTYSHKIRSLNLYTSLLLIELTHLLEQQSFSSADGCAENEMLRV
                     LDYIDKHFTSCTLKETAAHFGYNPVYLSNKLKMYFDASFQELVLKKKFDFALELIQET
                     SFSLEEIANRIGYKQTTSLFKLFKKYSTCTPNEWRNKH"
     gene            2521..3735
                     /locus_tag="IV334_000942"
     CDS             2521..3735
                     /locus_tag="IV334_000942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP8943032.1"
                     /translation="MLMNKKMMKFSLLSVSLILTSAGAVTSAIPSMSATYATRSLSAV
                     ELLTTAPSFMVTIFVLISSFIARKIGQKQTVIIGLVIASICGILPLFSQSYSIVLASR
                     AGLGIGFGLINSLAVSMIAAFYHGDERASMIGFQSAFQGFGAALMTFVAGQLVQFGWQ
                     YTFLVYAISLPILVLFVLFVPTPETTELQSHGTAPTRQVIKTNPKMISYGIFLLLVIV
                     VYNAVSIKLATVLTTYSLGNETNAANILTIMQLASMAAGVCFGWLQTKAHRYMLTLSL
                     LLMGIGFVLIATVINLYVIGFGAILTGISFSLFIPYLFNQVSIQAPKYAESFNTSILL
                     VGANLGTFIAPYGLLFLSFASLGSKTLAVFVNGSILLLISALISLLVVHRTNRKTVIR
                     VDEEIKEQLTTK"
     gene            3757..5772
                     /locus_tag="IV334_000943"
     CDS             3757..5772
                     /locus_tag="IV334_000943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 78 glycoside hydrolase catalytic domain"
                     /protein_id="HAP8943033.1"
                     /translation="MTNEKHKDRWLWYPGDFEIRHGLLQNFQREERGFDWPAYWYMDD
                     CHRNVKFKRYYFLDQPSMFKVTIQGVGYVEINGQKHPCGKWLTCPAGKAKIRIFVGHT
                     SGLPAMFIEGDEVKSDIGWTASNFIEEYPAGWSPLYVDIAKDPNQIYYQKECVHPVTE
                     TEIGGGILFDFGRAVNGCVELISLDDHIESITLCYGESDVEAMDIDYCYYKQIGMTRK
                     MKARKRAFRYLFIPEVSTGMVQLQVFHEFLSLSKQAAFSSDSSLLNQIWSVSEEAFKL
                     CSDLFFIDGIKRDRWIWSGDAYQSFMINQYLFFDEEINKRTILALRGQNEIKQHMNTI
                     VDYSILWLIGIENHYMMTKDYEFLRIVYPKMQSLMRYLMEQTNELGFIYGRAKDWIFI
                     DWSEMDKEGTLAAEQVLLLKAYHSMITCGDILAETYGENENYHTHLYQEKYQLLKQNL
                     FDYFWDEELGAFIDCYESGRRNVTRHANIFAVLFDFANKDQQQSILKNVLLNDTITQI
                     TTPYFKFYEQDALCKLGQTNIVYQTILDYWGGMLSHDAVTFWEEYDPTQTGNEKYSMY
                     GDPYGKSLCHAWGASPIYLLGRYFIGLYPTKPGYEEFMIQPNLDSFETLNCTLPIKDG
                     KISLEKTKDKIVITSDRTGGTVKMNGKSYPLLAKQPLVLTSYSKINA"
     gene            complement(5855..7228)
                     /locus_tag="IV334_000944"
     CDS             complement(5855..7228)
                     /locus_tag="IV334_000944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HAP8943034.1"
                     /translation="MDKLLNLFVSIVSQPSILVSLIALLGLVLQKKKFSEVIQGTIKT
                     FVGFLVLIGGAGLISNSLTPFASMFQTALHTQGVVPSNEAVVAIALQQYGTPTALIML
                     VGMIVNILLARFTRYKFIFLTGQAMLYVSCLTAVILVSAGFTANLWMILLGGVFEGTL
                     LTITPALCQPFMKNITGNNQVALGHTGNIGYASSGWIGKFFGNKDRSTEDIKIPKSFS
                     FLRDSTVSIMLLMSIVYVALALLAGPGFVERELSNGDNAVIFALIQAGTFTAGFVIVL
                     QGVRMVLAEIVPAFQGIAKKLVPDSKPALDVPIVFPYAPNAVLIGFFVSFFVGTLSMF
                     AMIGLQTTVIIPGVVGHFFCGAASGVFGNSTGGRRGAVLGSAFNSLLISWLPLFILPV
                     LGDLKIASSTFADTDYLVPGLVLGKLGEYGQIALTVGIIAFFLLVVLSSFLFAGKRTN
                     IVSKEQN"
     gene            complement(7254..7538)
                     /locus_tag="IV334_000945"
     CDS             complement(7254..7538)
                     /locus_tag="IV334_000945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAP8943035.1"
                     /translation="MKIFAVCQSGLGTSFMVEMNIKQALADLGADVDEFEVSHTDVGS
                     VSSDMADYFFIEHSLKDTLTSIPDEKLVPLQSIIDSEEVKEKVTKILSKE"
     gene            complement(7540..9555)
                     /locus_tag="IV334_000946"
     CDS             complement(7540..9555)
                     /locus_tag="IV334_000946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HAP8943036.1"
                     /translation="MNYKISYLLKTPREISLEKAAEIIEVNTSEAIKQLTPFAEYVDD
                     TRIRIKKFRAIDWAEKFLDITRFEAVYTEQERRDLLYLMVYSTATSVSVFHFQDFLQV
                     SKGTVLSDIKKIRNELEVEDCQLSYTRKTGFRLLGSEAILRREAKNRTARLMQSLAGR
                     FGLTYLTATLSIDYYAKVRDLLDKTISLSDLRFAPNRMDELAYYLSLTTIRIGKTSME
                     LVKDAELLASLAVTDISRQVLTGLGYSEHQNSEIIFFSLCLASIVEGDVQEPALDFLL
                     ECSSEVIHRMEFLMAVEFASFRELLMNVFYHLVPAYFRISYSFYLPNVMIDQIKHQYA
                     SIYEMTRKALLPLERRIGKSIPEEEIGFFTILFGGEIRKADAEERNRKIRAVIVCPSG
                     ISSSLILKSELQQLFPMIFFTETNSADRLHEVDETSYDIIFSTVPLTLTDKKKSLYVL
                     HPIMTDLEKNQLINQVQRDWLIPGFSMPSAQELLSALLPYIELKEGVDEEKLYQVLNR
                     KMNKLLEKKEDQRPMLSELLTEEMIQLSDQPKNWQEAITFAAQPLLSENKIEESYIKA
                     MIERVEQYGAFIHIGDHIALPHARPEDGVNEVGMSLLKLQQPVDLLDNPAHPISLFIC
                     LAAIDNEAHLRALANLTKLLSNKQNLQDLLHASTKEEILAIIRKGDE"
     gene            complement(9734..10039)
                     /locus_tag="IV334_000947"
     CDS             complement(9734..10039)
                     /locus_tag="IV334_000947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA processing protein"
                     /protein_id="HAP8943037.1"
                     /translation="MTNYDGEKHKRLRGSMAPKTEEEVALAGTNSGETEDKEESLAGT
                     NSGETENKETALSGSNSVEEETKPNEPNHFEKDIEKAVEDVKEKFEELTSKNKHDHH"
     gene            complement(10128..11159)
                     /locus_tag="IV334_000948"
     CDS             complement(10128..11159)
                     /locus_tag="IV334_000948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dehydrogenase"
                     /protein_id="HAP8943038.1"
                     /translation="MLTIAYLGNGKSTNRYHLPFSRKLTNKINIKKIFSPSEPVWKTF
                     EDIEYTDQLSDLWDDPEIQLVVITTPSQFHYEYGKMALEHGKNVLVEKPFAETSKEAE
                     ELFALAKEKGLFIQCYQNRRFDSDFLTVQKVIESGKLGDILEVEMHYDYFRPEVPENT
                     HEFSTANSYLYGHACHTVDQVLSYFGEPDDISYDVRQLLGKGRMNDYFDLDFFYGNMK
                     ISIKSSYFRIKERPSFVVYGKKGMFVKQTKDRQEEHLKMFYMPDHEDFGIDLPEHYGV
                     LTYIDEQGSYHEEKVISEVGDYSRVYEGIYETLVNGAEKVIKDEETILQMKILEEGIK
                     SIQESEGKI"
     gene            11366..12241
                     /locus_tag="IV334_000949"
     CDS             11366..12241
                     /locus_tag="IV334_000949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HAP8943039.1"
                     /translation="MLIEEKLTKQELFTTTEKRIVDYIRRNIEAAVYMTIEELAKATY
                     TSHSAIIRLCKKMGFSGFKEFRFELGREVHQLIAQFNQTIDPNFPFSSEDSTQQIAKK
                     MADLSIQSIKKAQLQIEHEDLDQIGKVLNKAQRIFLFAKGDSQITARKFQNKMVKLNK
                     FLIMAEEYSDSSWNAANLTSEDCAIFISYSGRIHHYERIMTYLKHVEAPTLLITGNQH
                     SEMAKQADMCLVISQEEYDFAKVATFSSQIAFDYVLNTLFSVIYSQNFEENIRNLKNK
                     QELMQKGLLKEYRKT"
     gene            complement(12393..13235)
                     /locus_tag="IV334_000950"
     CDS             complement(12393..13235)
                     /locus_tag="IV334_000950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HAP8943040.1"
                     /translation="MKPKKRKLLDLLMLFLLLSGIGILAYPFVSDALNNYLDQQIISH
                     YQQQAVKENEEVMAKIQENMTKKNQQLAKEGGNPGADPFTKKKEPAKKDRTYFEKHTI
                     GILTIPEINVHLPIFDETNTVLLEKGASLLEGTSYPTGGENTHAVISSHRGLPQAKLF
                     TDLPKLTLGDSFYIEINGRTLAYQVDQIKTVEPTDTKDLRIEKGQDFVTLLTCTPYMV
                     NSHRLLVRGHRVPYHAKEVQKEVQKVSENKRFFLYGLLAACILIFLLLIYLFRQSFKI
                     RNRS"
     gene            complement(13327..15204)
                     /locus_tag="IV334_000951"
     CDS             complement(13327..15204)
                     /locus_tag="IV334_000951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HAP8943041.1"
                     /translation="MKNHKKINVMLGVLFLILPLLTNSFGAKKVFAEETAAQVILHKK
                     KMTDLPDPLIQNSGKEMSEFDQYQGLADISFSVYNVTQEFYAQRDKGASVDAAKQAVQ
                     SLTPGTPVASGTTDADGNVTLSLPKKQNGKDAVYTIKEEPKDGVSAAANMVLAFPVYE
                     MIKQADGSYKYGTEELDTIHLYPKNTVGNDGTLKVTKIGTAENEALNGAEFIISKEEG
                     TPSVKKYIQSVTDGLYTWTTDQTKAKHFITGHSYDIGNNDFAEASIEKGQLIVNHLEV
                     GKYNLEEVKAPDNAEMIEKQTITPFEILANSQTPVEKTIKNDTSKVDKTTPQLNGKDV
                     AIGEKIQYEISVNIPLGIADKEGTQNKYTTFKLIDTHDAALTFDNDSSGTYAYALYDG
                     NKEIDPVNYSVTEQTDGFTVSVDPNYIPSLTPGGTLKFVYYMHLNEKADPTKGFSNQA
                     NVDNGHTNDQTPPSVDVVTGGKRFVKVDGDVTSDQTLAGAEFVVRDQDSDTAKYLSID
                     PSTKAVSWVSAKESATVFTTTSNGLIDVTGLKYGTYYLEETKAPEKYVPLTNRVAFTI
                     DEQSYVTAGQLISPEKIPNKHKGTLPSTGGKGIYVYIGAGVVLLLIAGLYFARRKHSQ
                     I"
     gene            complement(15201..16622)
                     /locus_tag="IV334_000952"
     CDS             complement(15201..16622)
                     /locus_tag="IV334_000952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HAP8943042.1"
                     /translation="MKKLGWLSMCLFLLLFKPAFTQVATETETEMVQITLHKLLFPNG
                     QLPKNHPNDGQEKALLQTYRGLNGVTFQVYDVTDSFYHLREKGKTVEEAQAEIAKNGA
                     SSGMFTAEATTTTLNNEDGIASFSLAAKDQEKRDKAYLFIESKVPEVVKEKAENMVVV
                     LPVHGQNNQKLSTIHLYPKNEENDYPDPPFEKVLEEPRNDFTIGEKITYSLHTTIPVN
                     ILDYQKFELSDSADEALTFLPNSLTISSNGEKLTEGFVIHKKPHGFDVLFSIPSLEKY
                     AGKKLTISYQMQLSSTAQANKEINNNGTLDFGFGVSTKKVSVYTGSKQFVKIETNKPD
                     KRLAGAVFLIKNKAGNYLQQTANGYKWTKNESDALHLISDKNGAFSISGLKTGSYRLK
                     EIEAPSGYILSETEIPFTISTFLSEDKEADSILKVVNKKENSRPFLPKTNETKNTLLG
                     VVGMVFASFAIWLFIKKRTGVKK"
     gene            complement(16625..20014)
                     /locus_tag="IV334_000953"
     CDS             complement(16625..20014)
                     /locus_tag="IV334_000953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HAP8943043.1"
                     /translation="MTTTGKKLKVIFMLIILSLSNFVPLSAIADTTDDPTVLETISAE
                     VISDQSGKKALNIKLNANNTSAEKIEKEIGLVENYLSDVERKEGDGYAYQVNSGKITL
                     EISSNTKQTIDLSFPIDPALYHSQANKLIVDNKEYDIIDETENKKDTDVSVPKPDEIE
                     EESSKENENSVSPFTLPTLSLPAVSVPSNQTIPTEYTTDDQGTYPKASWQPTGNTNVL
                     DHQGNKNGTNQWDGINSWNGDPNDRTHSYIEYGGTGNQADYAIRKYAKETSTPGLFDV
                     YLNARGNVQKDITPLDLVLVVDWSGSMNDNNRIGEVKIGVDRFVDTLADSGITDKINM
                     GYVGYSSEGYSYSNGAVQMGSFDSVKNQVKSITPSRTNGGTFTQKALRDAGSMLSVPN
                     GHKKVIVLLTDGVPTFSYKVQRVHAQSSSNYYGTQFSNTQDRPGNTSLISRIYDAPDQ
                     NNLSRRIDSTFIATIGEAMALKERGIEIHGLGIQLQSDPAAGLSKAEVESRMRQMVSS
                     DEKGDLYYESADHATDISEYLAKKAVQISATVSNGQINDPIAEPFIYQPGTLSVKSVG
                     TSPTTVTPSISIEGNTIKSNQIYLGKDQEIQIHYQVRIQTENEDFHPNFWYQMNGRTT
                     FQPNIDTNELAEFGIPSAKAPGVSLHIKKLWEEFDNNLADRPDQVTFEIQREHTTNAA
                     AWKNGYIRIIKPAKDTTNTWERADIDKLSANSGESYQEILSLPQYNNQGQAFSYQTIK
                     ELPVPGYDSQQIDAMTWKNTKQFTPLNLKITKNSSTGEKDLIGAVFKLTGDSIDTLLT
                     DHGDGTYSLPENVKLQKEMTYTLTETKAPEGHGLSKKTTWEIKIASDGTVTIDGKTVT
                     TSDDTIQLTIENPFVEVPVAVRKYAMQGTDKEINLKGAAFSLQKKEANGTYQPIDSQT
                     TNEKGLASFDSLTPGKYRVVETAGPAGYDTSPGNYEFQIDKYGKIIYTGKNTEMTNNV
                     WTLTHQNRLKAFDLTVHKKEDNGQTLKGAKFRLQGPEMDLESPKDGQETDTFLFENLK
                     PGTYTLTETFTPEGYQGLKEPVTIVIHEDGSIQVDGQDHESVLSPGAKNNQISLDITN
                     QAKVPLPETGGIGRLGIYLVGMIGCAFSIWYLFLKKERGGS"
     gene            20271..21662
                     /locus_tag="IV334_000954"
     CDS             20271..21662
                     /locus_tag="IV334_000954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HAP8943044.1"
                     /translation="MEKIIRILSISDKWMTKKELAKRIGSSESTFVRYIEEIKIRWGT
                     DVTIQSSQKLGYRLEHLNVPAYLNVLMEMSQSSTNSRLLNEIIVNPGKTIEYYCEKIS
                     ISRSSFTRKLKQCNQILESYLLKVIVDQGFQLTSLKSELQLRIFVTFFFLSYYGRRTL
                     PFPIEKKKIKEIMVKNNCQMNLFQEESPYEQSFFIMYFVVHLMRESQGTPFEWKAGDS
                     YVQLINIDKKDFLILKNLLGKLKYSSYIKVVNYFVNTIFSEFSEKEKNEISEQIRKNM
                     SYCSFSSADCKEKNEEYVVQLLTQIYFFSRIFPYDTTHLTIRATYFAQQMQHRQIRLY
                     AGFRAELQFYSTLLNQNLIYYLSSYIFWMLIGNPEIHHYTSDKKILIISDLSLRHSQY
                     IEEYISDILAVHKIHSETTAITEDQLSKYNLLEYDLIVTNQPILNAHVPHILIDDSVS
                     FANEEELIRLFEL"
     gene            complement(21735..22382)
                     /locus_tag="IV334_000955"
     CDS             complement(21735..22382)
                     /locus_tag="IV334_000955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAP8943045.1"
                     /translation="MTMKGYPRIIRDKGEMWMPKETFFNLSNAKKQRIDDLLLEIFCS
                     QHISQVKVSEIVDKMGMSRGAFYKYFQDLEDAYLYTIKSSSHIVHQDILKFISENKQH
                     FFLGIEKYLEWCCDLDHESIYWQRLYLLTQRNDQQTTKRRELSADSPMNEKWLELLRI
                     NHFSIQSSEEAVSFLYFSMTLVMNTLTDLIANNWTKEELLKDFRYKSRWIAEGIQ"
     gene            complement(22390..23076)
                     /locus_tag="IV334_000956"
     CDS             complement(22390..23076)
                     /locus_tag="IV334_000956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002366355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP8943046.1"
                     /translation="MKKILTMKKVTKTFGSGHTKVSALKAIDFYVKEGEFVSIIGPSG
                     SGKSTFLTISGGLQTPTSGEIVINGHSLTELPEKKRADLRFKEIGFILQSSNLIPFLT
                     VKEQFELVDQVTSKKEDSTKIDSLLQSLDIQSLKNSYPKDLSGGERQRVAIARALYNE
                     PSLILADEPTASLDTDHAYEVVKLLVKEAHEKQKATIMVTHDVRMIQWSDRVYRMEDG
                     YLTEEEKKKE"
     gene            complement(23082..24155)
                     /locus_tag="IV334_000957"
     CDS             complement(23082..24155)
                     /locus_tag="IV334_000957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HAP8943047.1"
                     /translation="MFLAIKEIRYSKLRYGLIVGIMFLIAYVVFMLSGLANGLSEEFK
                     KAIDDWDAQEIVLSEDANQVFAASQLTRGDLKYIEGGEKAPIGLYSGAIKGKNKENVT
                     VFGTTKEAFLLPKLTKGHEFKTKNEIIISQNLADSGYKIGDTIKIGSYDDPLTIVGIF
                     PETYYTVSPVIYASLDTWTALKYGQQPFASEEEQPINGIVIKDSAKISKEKASKGLQL
                     LTIGTFIESIPGYSAQNMTLNAMIYFLFLVVAAVVGIFMYVMTLQKTAIFGVMKAQGI
                     KNFFIAKSLVAQSFIVGVVGVLLALLFAYLTSLILPSAMPFAVFWSQWLLYSGILIIV
                     AMLGGLFSIRTITKVDPITAIGG"
     gene            24377..25051
                     /locus_tag="IV334_000958"
     CDS             24377..25051
                     /locus_tag="IV334_000958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP8943048.1"
                     /translation="MIKLLVVEDDKVLSNNIKEILAAIGEITQVYDGSEGLFELESGV
                     YDLAILDLMMPEMNGYEVLAKIRKEKIQTPVLILTAKDSLEDKIEGFERGADDYLTKP
                     FYREELIMRVKALLKRSLGLFDENVLKYKDISCDLKSNEVLYKEQVLPIQGKEFELLV
                     YFLQNKGIILTKEQIFDRIWGFDSDTTITVIEVYMSNLRKHLRSTGLDKEIKTLRNVG
                     YILQRV"
     gene            25056..26396
                     /locus_tag="IV334_000959"
     CDS             25056..26396
                     /locus_tag="IV334_000959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAP8943049.1"
                     /translation="MNGDITRKQRSRFFATNVAAFAFIFFLLGFITLQILNQSAYRET
                     DLSLEKINFDSRTVQLEIQRYQQGDPFLNPTQTEQNRTNMEQPLMEPSNDRFNTQVIL
                     WSKNGEILNKAAIGGRFNQIKELKMDTKNLNTIQNIELNEDSGSSALSFRSITKKAET
                     NQDGVAYIQILENTNQIKNSLATFRTIMILCMIVFWILSIGISYYLSSLSMRPIILSW
                     RRQKEFVENASHELRTPLTIIQNSLEHLFTKPNHTIIEESESIAQALSETRRLTGLTS
                     DLLTIARNDSDQQLLSKQMINTQEYIEQLVKPFQEMAIIDGKEFILHNDATLEVPIDE
                     KKIHQVLVILLDNALKYTNPEDKIILDSSVNHKEWLIHVKNTGPSISEEDQARIFDRF
                     YREDQSRAKETGGYGLGLAIAKQVIEQHHGQISVSNYLPQGVDFKVQLPLKPKK"
     gene            complement(26545..27531)
                     /locus_tag="IV334_000960"
     CDS             complement(26545..27531)
                     /locus_tag="IV334_000960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2382 domain-containing protein"
                     /protein_id="HAP8943050.1"
                     /translation="MEEKNLTVIGSYSTREEALSVIERLRNEGYERDDIVIYTTDEAA
                     SRLGFDGLSGIDVETDENRMDGEEDRSLWEKIKDTFSFDTYDSETATPENDPLYQYRG
                     DISDGKFVITVKGYRQPETTEDTMDTQDTVSPASTMGTGLETGTGTDPMDVGGTTGFQ
                     NTTDPLNMTPGTDPMDVPGETDTMGTSKTEDIDRKPNLDDDTIQLKEEKLDVNTHDVT
                     TGEVDIHKHVVNDTETVEVPVKREEIVIERKPVTDQSSQGTDEKLEDDTITIPIKEEQ
                     VDVSKHTVIREEVGIHKEEHEDVEKVTEDVSREELDIDTSGDVHIEDRNKKS"
     gene            28255..29286
                     /locus_tag="IV334_000961"
     CDS             28255..29286
                     /locus_tag="IV334_000961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HAP8943051.1"
                     /translation="MDSKTRKQLQEKLKQRTHLLPSVFQQFLEENRSSLSLGSRYEYA
                     KDFHLFTDYLRKVFSEEVDDQLIIHLEKQDYQNFLAKIYRHTRSFQTTANQETEQLFE
                     NSYYGISRKQYSLNHFLRFLYQKGLIEEEISLLGTSLPETTKAAPRYIDAELFKDFEH
                     LFIPIEGFQTSREASFEEKNRPRNLAITAILLYCGLKIHEVVELKRKDVSLTKQILNL
                     NRKENRLNKVPIPKEAMPFLENYFKLTAQNTDENSFVFRSSHDQQISPRTIRQILSKI
                     TVYKNVSPELCRKTYRYYAELYLDDADAVNYLCGNRYLSSHSFQDIWEKMVDFSYHEL
                     AAFVRVSSI"
     gene            29395..>29819
                     /locus_tag="IV334_000962"
     CDS             29395..>29819
                     /locus_tag="IV334_000962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase"
                     /protein_id="HAP8943052.1"
                     /translation="MMETITYSPLNLFTNFQEAAANFSETPIIFDEPLSAFPELGLET
                     TYKKCCESVLKRAYQLAHLGVKSGDKVIIYKSSAFDTYLLAVSVSYLGAVPVMTSYHL
                     PTTTMEVFIDRLEDPFILFDDETKKRVREISNGTKSKQIP"
BASE COUNT         8830 a         5595 c         5365 g        10029 t
ORIGIN      
        1 ttaattttaa ccattctgtt tatttgctta cctattagat atcaataaaa aactgcttaa
       61 cagctatttc agctgttaag cagttttagg atttttagtt catattcatt gtttcttata
      121 atacagtgtt caatacttct cttaaagcac ttgttgattt gctgaacatt gctttttctt
      181 cttcgttgat tgataattca acaacttcac gaacaccatt ttcgtcaacg attgatggca
      241 cacctgtaaa gatatctttt tcgtcgtatt caccagttaa ataagcagat acaggcaata
      301 cagcttgttc gttattcaag atagctttta cgatacgtgc agtactcata ccaataccat
      361 agtaagttgc ttttttacga tcaatgatct cataagcagc attacggact ttatcagcaa
      421 tcacatctaa ttcatcttgg gcaatacgat ggtctttttc tacaatttca aacactggtt
      481 taccaccgac agttgtatgt gaccatgcag caacttctga atcaccatgt tcacctaaga
      541 tatagccgtg gacgctgcgt gggtcaactt ttaatttcaa tgcgatttct ttacggaaac
      601 gagttgtatc caatgttgtt ccagtaccga taacacgtga tgttggcaga ccagattcgt
      661 tccaagcgat atatgtcaaa atatctactg ggtttgaagc aacaacgata ataccgtcga
      721 atcctgatcc catgatttct tttacgattt ggcgcatgat tgacgcattt gttttaacta
      781 aatctaaacg agattgacca ggtttttggt tgataccagc tgtgataaca acgatatcag
      841 catctttaca ttcttcatag ccacctgacc atacagccac attttcatcg ccccaagcca
      901 taccatctaa aaggtccaat gcttcccctt ctgctttttc ttgattcaca tcgatcaata
      961 ctaattcatt agagattcct tggttgatca ttgcataagc gatacttgtt ccaacaaaac
     1021 ctgtaccgac gattactact ttacgacttg tttttttcat gaataaacag ctccttttca
     1081 taactatttg ttactttcat gaaaagtata gtcttgttta ttcattttat caacatgtta
     1141 attataatag ttgttattat aatttttgat ggaaacgaat tcctgaatac gcataacaga
     1201 taaatgacaa atgtttacac ctatatcccc tgataataca accaaaagtt tagaactgaa
     1261 aataaatcca aaaaaattcg ctgcaacgat ataataagac agcaatcgca cctaaaacaa
     1321 ttttccttca tatgctttag tgaaagattt tcttcttaat gtttatttct ccattcattg
     1381 ggagtacatg ttgaatactt cttgaataat ttgaataaag aagtcgtttg tttatatccg
     1441 atacgattag cgatttcttc caacgaaaaa ctcgtttctt gaatcagttc caacgcaaag
     1501 tcgaattttt tctttagaac cagttcttga aaggaagcat caaaatacat tttcaattta
     1561 ttactaagat agaccggatt gtagccaaaa tgagctgctg tttcttttaa cgtacaagaa
     1621 gtaaaatgct tatcgatata atctaatact ctaagcatct cattttctgc gcatccatct
     1681 gctgatgaaa agctttgttg ttctaataaa tgagtcaatt cgatcagtaa tagtgatgta
     1741 tagagattta acgaacggat cttatgactg tatgtatcaa aataaatcaa cagcatattt
     1801 tcaatcaaat gcgtcaaacg acttgttttt ccgccgcgaa aatgaatgaa ttgtgtatgt
     1861 tccccttttt cttttgatgc atttatcaaa aaagaagaaa gtagattatt tgtgcttgcc
     1921 aaatgattca atatctctgt agataaggtt tcttctttta ataagatgtt gattaaaata
     1981 tcctcttgcc ctgtgtattc gattctttga gtagcttctt tatcaatgat caccacgtca
     2041 ctttcttcca tctctatcct ttctccatta ataatttgta aacatgtacc gctatacata
     2101 taattgattt caacaaaatc atgtgtatgc tcgggaactt ccgcatatcg acggtgttta
     2161 ctgatatgaa tatttccctg ttgaaataga tcccctcggt ggatttttgg cggtgtctga
     2221 agatggtaag ctaagtttaa atcattgata ttcattcctt gttcacgctg cagagtttcg
     2281 attgattctt cgttacgcaa aaaaaatgtc taattcagct agttccaatt gactccctcc
     2341 tttttgtaag cggtttcttt cgcttattag ttcacgctgt actgctatta tatgcaaagc
     2401 tatttttggt ttcataaaga ctttctatgg ttgggacatg aacttcctgt gattccgcta
     2461 ttgagagaat aaccacttca tactatattt ttgataagcg cttacaaaaa tgaaaggaac
     2521 atgcttatga ataaaaagat gatgaaattt tctctgctgt cagtctctct tattttgact
     2581 tctgctggtg cagtcacgtc tgcaattccc agtatgtcag ctacttatgc cacgcgttct
     2641 ttatctgctg ttgaattatt aacgactgct ccctctttta tggtcactat ttttgttttg
     2701 attagtagtt ttattgctag gaaaatcgga caaaaacaaa ctgtgatcat cggattagtg
     2761 attgcatcga tttgtggtat cctccccctc ttctcacaaa gttacagtat tgtcttagct
     2821 tcaagagctg gactaggtat tggctttgga ttgatcaatt ctttggcagt cagcatgatt
     2881 gctgcctttt atcacggaga tgaacgagcc tctatgattg gttttcaaag tgcatttcaa
     2941 ggatttggtg cagctttaat gacatttgtt gcaggacagc tagtacagtt cggttggcaa
     3001 tatacctttt tagtctacgc catcagctta cctattcttg tcttgtttgt tttgtttgtt
     3061 cctacacctg aaacaactga acttcaatcg catggcacag ccccaacaag acaagtaatc
     3121 aaaacgaatc ctaaaatgat tagttatggt atttttctct tgctggtcat cgtggtatat
     3181 aatgctgtat cgattaaatt agccacagtc ctgacgactt attctctagg aaacgaaact
     3241 aatgcagcga atatcttaac aatcatgcaa ttggcaagta tggctgccgg agtttgtttt
     3301 ggctggcttc agacaaaagc acatcgctat atgcttacac tttctttact actgatggga
     3361 atcggctttg tattgattgc cactgtgata aacctgtatg tgattgggtt tggtgcgatc
     3421 ctaacaggaa tttctttttc gttattcatt ccttacttat tcaaccaagt aagtatccaa
     3481 gcgcccaagt acgcggagag tttcaatact tcaatcttat tggttggcgc taatttaggt
     3541 actttcattg caccatatgg tttgctcttt ctctcttttg cttctcttgg gagtaaaaca
     3601 cttgctgttt tcgtcaatgg cagtatcttg ctgttgatta gcgcgcttat tagccttcta
     3661 gtagtacaca ggacgaatag aaagacagtg ataagagtag atgaagaaat aaaagaacaa
     3721 ttgacaacaa aataaatttg atggaggtcg tgtaaaatga caaatgaaaa acataaagat
     3781 agatggcttt ggtatcctgg agactttgag atccgccatg gactgctaca aaatttccaa
     3841 cgggaagaac gaggttttga ctggccagcg tactggtaca tggatgattg ccatcggaac
     3901 gtcaagttta agagatatta ctttcttgat caaccttcca tgtttaaagt gacgatccaa
     3961 ggagttgggt atgtagaaat taatggacaa aaacatccat gtggaaaatg gctgacttgt
     4021 ccagctggta aagcaaaaat tcgtatcttt gtaggacata catcagggct accagcgatg
     4081 tttatcgagg gagacgaagt aaaatcagat attggctgga cagcaagtaa ttttattgaa
     4141 gaatatccag ctggctggag tccgctttat gtcgatatcg caaaagatcc aaatcaaatc
     4201 tattatcaaa aagaatgcgt ccatccagtc actgaaacgg agattggcgg aggaatctta
     4261 ttcgattttg ggcgagcagt gaatggctgt gtagaactaa tatctctgga tgaccatatc
     4321 gaatctatca ctctatgtta tggagagtcc gatgtagaag ctatggatat cgattattgc
     4381 tactataagc aaataggaat gacaagaaaa atgaaagctc gtaaacgcgc ttttcgctat
     4441 ttgtttatac cagaggtttc tactggaatg gttcaacttc aagtttttca tgaattttta
     4501 tctctttcga aacaagctgc tttttcaagc gattcttctt tactgaatca gatctggtca
     4561 gtatcagaag aagccttcaa gttatgtagt gatttgtttt ttatcgacgg tatcaaacga
     4621 gatcgctgga tttggtctgg agatgcgtat caatcattca tgatcaatca atatcttttt
     4681 tttgacgagg aaatcaataa acgaacgata ttagcattaa gaggacaaaa tgaaatcaag
     4741 cagcatatga atactatcgt tgactattcg atcttgtggt tgattggtat cgaaaaccac
     4801 tatatgatga cgaaagacta tgaattttta cgcatcgtct accctaagat gcaatcatta
     4861 atgagatact tgatggagca aacgaatgaa ttagggttta tttatgggag agcaaaagat
     4921 tggatattta ttgattggtc tgaaatggat aaagaaggaa cgcttgctgc agaacaagtc
     4981 ttattactga aagcctatca ttccatgatc acttgcggag atatcttagc tgaaacatat
     5041 ggagagaacg aaaattatca tactcatctc tatcaagaaa aatatcaact attgaaacaa
     5101 aacctatttg actacttttg ggatgaagaa cttggcgctt ttatcgattg ctatgaatct
     5161 gggagaagaa atgtgacgcg tcatgcgaat attttcgctg ttttattcga ttttgctaac
     5221 aaagaccaac agcaatcgat cttaaagaat gtcttattga atgacactat cacacagatt
     5281 actactccat atttcaaatt ctatgaacaa gatgctttat gcaaactcgg acagacgaat
     5341 atcgtctacc aaacgattct tgattattgg ggagggatgt tatctcatga tgcggtaact
     5401 ttctgggaag aatacgaccc tactcaaaca ggaaatgaaa agtacagtat gtatggagat
     5461 ccttatggaa aaagcttatg ccatgcatgg ggagcaagcc caatctatct tttaggacgc
     5521 tattttatcg gtttgtatcc aacaaagcca ggttatgaag aatttatgat acaacctaat
     5581 ctagattcat ttgaaacact gaattgtacg ctacctatca aagatggaaa gatttcgctt
     5641 gaaaaaacaa aagataagat cgtgattact tctgatagaa caggcggaac agtgaaaatg
     5701 aatggtaaaa gttatcctct tctagcaaaa cagccacttg tcttaacaag ctattcaaaa
     5761 ataaatgcat aaaacaaaaa actgcttgcg acatatcaga agactttaaa tcttcttctg
     5821 atattgtcca agcaattttt attttgaagt ttgcttaatt ctgttcttta gaaacaatgt
     5881 ttgttctttt tccggcaaaa agaaagctag ataatacgac aagtaaaaag aaagcgatga
     5941 ttccaactgt caaggctatt tgaccgtatt ctcctagttt cccaagtaca agtcctggta
     6001 ctagataatc agtatcggca aatgtagaag aagcgatttt aagatctcct aatactggaa
     6061 ggataaataa tggcagccaa ctaatcaata agctattgaa agcagaacct aataccgccc
     6121 ctcttctacc gccagtcgag ttgccgaaga caccagaagc agctccgcag aagaaatgtc
     6181 ctactacccc aggaatgata actgtagttt gcaagcctat catagcaaac atggatagtg
     6241 ttcctacaaa gaagctaaca aaaaagccaa ttaatacagc attaggtgca tatggaaaaa
     6301 cgataggaac atctaatgct ggtttagaat cgggtacgag ttttttagca atcccttgga
     6361 atgctggaac gatttctgcc aacaccattc gaaccccttg aaggacgata acaaacccag
     6421 ctgtgaatgt tccagcttgg atcagtgcaa agattacagc gttgtctcca ttgcttagtt
     6481 ctctttcaac aaatccagga ccagctaaca aagcaagtgc gacatatacg atcgacatca
     6541 agagcatgat cgatacagta gaatctcgca aaaagctgaa acttttagga atctttatgt
     6601 cctctgtaga acggtctttg tttccaaaaa actttccgat ccatccacta gaagcataac
     6661 cgatatttcc ggtatgccct aacgcaactt gattgtttcc agtgatgttc ttcataaacg
     6721 gttgacaaag agcaggcgta atagttaata gtgttccttc gaagaccccg cctaataaga
     6781 tcatccacaa atttgcagta aaccccgcgc ttactaaaat gactgctgtt agacaagaaa
     6841 cataaagcat tgcttgacct gtaagaaaaa tgaatttata tcttgtgaat ctagcaagta
     6901 aaatattcac gatcatccct actagcatga tcaaagcagt aggtgtaccg tattgttgca
     6961 aagcaatcgc cactactgct tcattgctag gaactactcc ttgagtatgc agagctgttt
     7021 ggaacatcga agcaaaaggg gttagtgaat tactgattaa accagcaccg ccgatcagta
     7081 ctaggaatcc gacaaatgtt ttgatagttc cttggatcac ttctgaaaat ttcttcttct
     7141 gaagaacgag accaagcagt gcaatcaaag aaacgaggat ggagggctga ctgacgattg
     7201 acacaaacaa attcaacaat ttatccattg atagttcctc ccttgaatca tttttattct
     7261 tttgacaaga tcttcgttac tttttctttt acttcttcgc tgtcaatgat cgattgcaaa
     7321 ggtacgagct tttcatcagg tatagaagtt agcgtatctt tcaaagaatg ctcaatgaag
     7381 aaataatcag ccatatcgct agatacactt ccaacatccg tatgacttac ttcaaactcg
     7441 tccacatctg ctccaagatc tgcaagtgct tgtttgatgt tcatctctac cataaaactt
     7501 gttcctaatc cactttgaca tactgcaaaa attttcatat cattcgtctc cttttcttat
     7561 gatcgctaat atttcttctt ttgttgatgc gtgtaataaa tcttgaagat tttgtttatt
     7621 agaaagtagc tttgttaaat ttgccaaagc ccgaagatgt gcctcattat cgatagcagc
     7681 taaacagatg aacaaactaa tgggatgggc tggattatca agcagatcca caggttgttg
     7741 aagtttcagc aaagacatcc ctacttcgtt tacgccatct tctggacgag catgaggtaa
     7801 tgcgatgtga tcgccaatat ggataaacgc accatactgc tccacgcgct cgatcatagc
     7861 tttgatataa ctttcttcta ttttattttc tgataatagc ggctgtgcgg caaatgtgat
     7921 tgcttcttgc cagttttttg gttgatccga taattggatc atttcttctg ttaataattc
     7981 agatagcata ggacgttgat cctccttctt ttctaaaagt ttgttcattt tacgatttaa
     8041 tacttgatag agcttttctt cgtctacgcc ttcttttaat tcgatataag gcagtagcgc
     8101 cgacaacaat tcttgggcag aaggcattga aaatcctggt atcagccaat ctctttgtac
     8161 ttgattgatc agctgatttt tttccaagtc agtcatgatt ggatgcagaa catagagaga
     8221 ctttttctta tcagtaagtg tgagaggtac agtagaaaaa ataatatcgt aagaagtttc
     8281 gtcaacctcg tgtaagcggt cagctgaatt agtctctgta aaaaagatca tcgggaataa
     8341 ttgctgaagt tcacttttta agatcaatga agagctaatg ccacttggac agacgatcac
     8401 ggctcttatt tttctatttc tttcttctgc gtccgctttt ctgatctctc cgccaaatag
     8461 tatagtgaaa aaaccaatct cttcttcagg gatgctcttc cctattcgtc tttctaacgg
     8521 aagtaatgct tttcttgtca tttcgtagat ggaagcatat tgatgcttaa tttgatcgat
     8581 catcacatta ggtaaataaa aagaatagga aatacgaaaa taggcaggga ccaagtgata
     8641 aaagacattc atcaataatt cacgaaacga agcaaattct acggccatca ggaattccat
     8701 gcgatggatg acttctgaag aacattccag caaaaaatca agtgcaggct cctggacatc
     8761 tccttcaacg atcgatgcta agcaaagaga gaaaaatata atctccgaat tttgatgttc
     8821 ggaatagcca aggccagtca atacttgtcg actaatgtca gtgactgcaa gagaagccaa
     8881 cagttcagca tcttttacca gttccatgga tgtttttcca attcgaatgg ttgtcaaact
     8941 caaataataa gctaattcat ccattcgatt tggtgcgaat cgaagatctg atagtgaaat
     9001 cgttttatct agcaaatctc taactttggc gtaataatca atggaaaggg ttgcggttaa
     9061 atacgtgagc ccaaatcttc cagcaagtga ctgcatcaaa cgagcagtgc gattttttgc
     9121 ttctcttctt aatattgctt ctgaccctag cagcctaaaa ccagttttcc tagtatacga
     9181 cagctgacaa tcttccactt ctaattcatt tcttatcttt ttgatatctg acaggactgt
     9241 ccctttagaa acctgaagaa aatcttgaaa atgaaaaaca gaaacagacg ttgcagtcga
     9301 ataaaccatc aaataaagaa gatctcttcg ctcttgttcg gtatacacag cttcaaatcg
     9361 agtaatatcc aaaaattttt ctgcccagtc gattgctcgg aatttcttta tacggatacg
     9421 ggtatcatcc acatattcgg caaaaggagt taattgtttg atcgcctcgc ttgtatttac
     9481 ttcgatgatc tctgctgctt tttctagact gatttctctt ggtgttttca ggagataact
     9541 aattttgtag ttcacagcgc tgttcacctc ctacttatat tctagtatgg tagcgctttt
     9601 aaatcgaggg gtgtttggta caacttacag ttggattttt tttacgactg taccgaacga
     9661 tttctatcga atggatacag aaaaaaagca ggtgaccaaa tgtctcctgc ttttatgaat
     9721 tgatttcgtc cgatcaatga tgatcatgtt tattttttga ggtcagttct tcaaattttt
     9781 ctttgacatc ttcaaccgct ttttcgatat ctttttcaaa gtgattgggc tcattaggct
     9841 ttgtttcctc ttctacagaa tttgaaccag ataaagctgt ttctttattt tctgtttcac
     9901 cagagtttgt gccagctaat gattcttctt tgtcttctgt ttcaccagaa ttagttcctg
     9961 ctaaagccac ttcttcttct gttttagggg ccattgaacc tcttaatcgt ttgtgttttt
    10021 caccgtcata atttgtcatg ttggttcctc ctagtagttc tatacttttt catcgtacaa
    10081 taagacagtt tatgcgtcaa aaaacaaacc cgattcttac aaatatgtca tatttttcct
    10141 tctgattctt gaatgctttt gattccttct tccaaaattt tcatttgtaa gattgtttct
    10201 tcatctttga tgactttttc tgcgccatta actaatgtct cgtaaatccc ttcgtagaca
    10261 cggctgtaat caccaacctc ggaaattact ttttcttcat gataactccc ttgttcatct
    10321 atataggtca atacaccata atgttcgggc aaatcgatac caaagtcttc atgatcaggc
    10381 atgtaaaaca ttttcagatg ttcttcttgt cgatcttttg tttgtttcac gaacatccct
    10441 ttttttccgt aaacgacgaa acttggacgt tctttgatgc ggaaataact ggattttatc
    10501 gaaattttca tatttccata gaaaaaatcc agatcgaagt aatcattcat tcgtcctttc
    10561 cctaaaagtt gacgtacatc atagctgata tcatctggtt cgccgaaata agaaagaact
    10621 tgatctactg tatgacaagc atgtccatat aaatagctgt tcgcagtaga aaattcatgc
    10681 gtattttctg gtacttccgg tctgaaataa tcataatgca tttctacttc taaaatatcc
    10741 cctaattttc cgctttcgat tactttttgg actgtcaaga agtcagaatc gaaccgtctg
    10801 ttttggtaac attggataaa tagacctttt tcttttgcta atgcaaataa ctcttctgct
    10861 tcctttgacg tttctgcaaa aggtttctct actagtacat tcttaccatg ttcgagtgcc
    10921 atttttccat attcataatg aaattgacta ggtgtagtga tgacaactaa ttggatctca
    10981 ggatcatccc aaagatcaga cagctgatca gtatattcga tgtcttcgaa tgttttccac
    11041 actggttcag atggggaaaa aatttttttg atattgattt tgttcgtaag ttttcttgaa
    11101 aaaggcaaat gatagcgatt tgtactcttt ccattgccaa gataagcgat tgtaagcatg
    11161 agtggtgcct ccttttattg atgttttcag tatatcctag aaaagcgcag aaagtattta
    11221 taatagctat tctggtaaaa aagttcagaa aaacacttgt tatcattcgg tttggtgaaa
    11281 ttaatgttca aagtaaggat aaaaatcaaa aagatagtag cggaaccaag tgtttatcag
    11341 taagatagaa tttggaggtc agatcatgct tattgaagaa aaactaacaa aacaagagtt
    11401 gtttaccacg acagagaaac ggattgtcga ttacatccgt aggaatatcg aagctgcagt
    11461 ctacatgacg attgaagaat tagcaaaagc tacgtatact tcccattcag ctatcattcg
    11521 tttatgcaaa aaaatgggat tcagcggatt taaagaattc cgtttcgagc tggggcggga
    11581 agttcatcaa ttgattgctc agttcaatca aacgattgat cctaattttc ctttttcaag
    11641 tgaagatagc actcagcaaa ttgcaaagaa aatggcggat ttatcgatcc agtcaataaa
    11701 aaaagcacaa ttgcagattg aacatgaaga tttagatcaa atcggtaaag tattgaacaa
    11761 ggcacagcga atctttcttt ttgcaaaagg tgattctcaa atcacggcaa ggaaattcca
    11821 gaataaaatg gtcaagttga ataagttttt gatcatggct gaagaataca gtgattcttc
    11881 ttggaatgca gccaatttga ctagcgagga ttgcgccatt tttattagct acagcggtcg
    11941 catccatcat tatgaacgaa taatgaccta tttgaagcat gtagaggccc ctaccttgct
    12001 gattaccgga aatcagcatt cagaaatggc caaacaggct gatatgtgtc ttgttatatc
    12061 gcaggaggaa tatgattttg ctaaagtagc gactttctcc tctcaaattg ccttcgatta
    12121 cgtattaaat acgttattct cagtgatata ttctcaaaat tttgaagaga atatccgcaa
    12181 cttgaaaaac aaacaagaat tgatgcaaaa aggccttctt aaagaatatc ggaaaactta
    12241 agaaaagatc ctcatttagt aataagaaag atagtgtttg ataggctctc tttgatatca
    12301 tcataaaaac aggctgaagt tttctttttc aaacctcagc ctgtttgttt acggaacgat
    12361 tcgaatagat atattataga aatgaggaat agctaacttc tgtttcttat tttgaacgat
    12421 tgacgaaaaa gatatataag aaggaggaaa atcagtatac atgcagctaa caatccatac
    12481 aggaaaaatc gtttgttctc ggatactttc tgtacttctt tttgaacttc ttttgcatga
    12541 taaggaaccc ggtgccctct gacaagtaag cgatgactgt tgaccatata aggtgtacaa
    12601 gttaggagtg tcacgaaatc ctgccctttt tcgattctca gatcttttgt atcagtcggt
    12661 tctaccgtct tgatctgatc gacttgataa gcgagtgtcc gtccattgat ttcgatatag
    12721 aaagaatcac ctaaagtcag tttcggtaag tctgtgaaca actttgcttg aggcagtcct
    12781 cgatggctgg aaataacggc atgtgtattt tctccccccg taggataaga cgttccttcc
    12841 aataatgagg cacctttttc tagcagcaca gtattcgttt catcaaaaat tggcaaatgg
    12901 acattgatct cagggatcgt caaaataccg atcgtatgtt tctcaaaata ggttctatcc
    12961 tttttcgcag gttctttttt cttagtaaag ggatctgcac ctggatttcc accttctttt
    13021 gctaattgct ggtttttctt tgtcatgttt tcttggattt tagccatgac ttcttcattt
    13081 tctttgaccg cttgttgctg ataatgagag atgatctgct gatccagata attattcagc
    13141 gcgtcgctaa caaatggata agctagtatc cctattcctg agagaagcaa gaaaagcatc
    13201 agcagatcta gaagtttcct tttctttggt ttcatttgtg ttctccttgt gatgactgtt
    13261 tgataataaa agaaaaagga gcatttgcgt aatccgctgc tcctttttcc aattcttgca
    13321 aaaagactaa atctgactgt gcttgcgtct agcaaagtac agtccagcaa tcagtagaag
    13381 gactactcct gcaccgatat acacatagat tcccttaccg cctgttgaag gaagtgtacc
    13441 tttgtgttta tttggtattt tttcaggaga aatcaactgt cctgctgtta cataagattg
    13501 ttcatcgata gtaaatgcta cacggtttgt taatggaaca tatttttctg gcgctttcgt
    13561 ttcttccaga tagtacgtgc catattttag acctgtcaca tcgattaaac cgttacttgt
    13621 ggttgtaaaa accgttgctg attctttcgc cgatacccag ctgacggctt ttgtggatgg
    13681 gtcgatcgat aaatatttcg ctgtgtcact atcttgatca cgaacgacga attctgctcc
    13741 agcaagtgtt tggtctgatg taacgtcacc atctacttta acaaatcgtt tgcccccagt
    13801 aacgacatcg actgacggtg gtgtttgatc atttgtatgc ccgttatcga catttgcttg
    13861 gttagaaaat cctttggttg gatctgcttt ttcgttcaaa tgcatatagt aaacgaattt
    13921 caatgtaccg ccaggagtta atgaaggaat ataattcgga tcaactgaaa ccgtgaatcc
    13981 gtctgtttgc tcagtgacag aataatttac tgggtcgatt tctttatttc catcatataa
    14041 ggcataagca tacgttcctg aagaatcatt atcaaatgtt aaagcagcgt catgagtatc
    14101 gataagtttg aatgttgtgt acttgttttg cgttccttct ttatcagcga tacctaatgg
    14161 gatattgaca gaaatctcat attgaatttt ttcaccgatt gcgacatctt ttccattcaa
    14221 ttgaggtgtt gttttatcaa ctttagacgt atcatttttg atggtctttt ctactggtgt
    14281 ttggctattt gccaggatct caaaaggcgt gattgtttgc ttttcaatca tttccgcatt
    14341 atcaggagct tttacttctt ctaaattata ttttccaact tctaaatgat taacgatcaa
    14401 ctggcctttt tcaatagatg cctcggcaaa gtcattgttg ccgatgtcat aagaatgacc
    14461 agtaatgaaa tgtttggctt tggtttgatc agttgtccaa gtgtacaatc catctgtgac
    14521 actttggatg tattttttga cgcttggtgt tccttcttct ttagaaataa taaattctgc
    14581 tccatttagt gcttcgtttt cggcagtacc gatttttgta actttcaacg ttccatcatt
    14641 accgactgta tttttagggt agagatggat agtatctagt tcttctgtcc cgtatttata
    14701 agagccatct gcttgtttga tcatctcata tacagggaaa gctaaaacca tgtttgcggc
    14761 agctgacact ccgtcttttg gttcttcttt gatcgtgtag actgcatctt tcccattttg
    14821 ttttttaggt aaagataaag tgacatttcc atcagcatct gtcgttcctg aagcaactgg
    14881 tgtaccagga gtcaaagact ggactgcttg ttttgctgca tccacggacg ctcctttatc
    14941 tcgttgcgca taaaattctt gagtgacgtt ataaactgaa aatgaaatat cggctaatcc
    15001 ttggtattga tcgaattcgc tcatttcttt cccgctgttt tggattaaag gatcgggtaa
    15061 atcagtcatt ttctttttat gaaggatgac ttgagctgct gtctcctctg caaacacttt
    15121 ttttgcgccg aagctgtttg tgagtaatgg taaaataagg aaaaggactc ctaacataac
    15181 gtttattttt ttatgatttt tcattttttc actcctgttc tttttttgat aaacaaccag
    15241 attgcaaagc ttgcgaatac cataccaaca acgcctaaaa gtgtattttt cgtttcgttt
    15301 gtttttggaa gaaatggacg gctattttct tttttattga ctactttcaa tatactgtcc
    15361 gcctctttat cctcagaaag aaaagttgaa atggtaaacg gaatttctgt ttcacttaaa
    15421 atataaccag aaggtgcttc gatctctttt aatcgataac ttcctgtttt caacccggaa
    15481 attgaaaaag cgccattttt atcggaaatc aggtgaagcg catctgattc gttctttgtc
    15541 cacttgtatc cgttggctgt ttgctggagg taatttcctg ctttgttttt aataaggaat
    15601 actgcgccag ctaatcgttt atctggttta tttgtctcga ttttgacaaa ttgcttactc
    15661 cctgtatata cagagacttt ctttgtactg acaccaaaac caaaatccag tgttccgttg
    15721 ttgttgattt ccttgttcgc ctgtgctgta ctgcttagct gcatctgata agaaatggtc
    15781 agtttttttc cagcatattt ttccaacgaa gggatcgaaa ataaaacatc aaatccgtga
    15841 ggtttcttgt gtatgacaaa gccttctgtc agcttttctc cattcgatga aatcgttaaa
    15901 ctattaggta aaaacgttaa tgcttcatcc gcactatctg acaattcgaa cttttgatag
    15961 tcaaggatat ttacaggaat tgtcgtatgc aaggaataag tgattttttc accaatcgta
    16021 aaatcatttc taggctcttc taataccttc tcaaaaggtg gatcagggta gtcgttttct
    16081 tcatttttag gatacaaatg gatagttgaa agtttttgat tgttttgtcc atgtacagga
    16141 agaacaacta ccatattctc tgccttttct ttgacgactt ctggtacttt ggattcaatg
    16201 aaaagatacg ctttatctct tttttcttga tctttagcgg ccagagaaaa agaagcgata
    16261 ccatcttcgt tgttaagagt tgtagttgtt gcttctgcgg taaacatacc ggaagacgca
    16321 ccgttttttg cgatctctgc ttgtgcttct tctaccgttt tgcccttttc ccgtagatgg
    16381 taaaaagaat ctgtgacatc ataaacttgg aatgtgacac catttaatcc tcgatacgtt
    16441 tgtaataaag ctttttcttg tccgtcattt ggatgatttt tcggcagttg cccgtttggg
    16501 aaaagcaatt tgtgtaaagt aatctgaacc atttctgttt ctgtttctgt tgctacctga
    16561 gtaaaagctg gtttaaatag taacaagaag agacacatac taagccaacc aagttttttc
    16621 atatttagct gccccctctt tcttttttca aaaaaagata ccaaatagaa aacgcacaac
    16681 caatcatccc tactagatag attcctaaac ggccaattcc tcccgtttca ggtaatggta
    16741 cttttgcctg attcgtgatg tctaaagaaa tctggttgtt tttggctcct ggtgacagaa
    16801 cagattcatg atcttgtcca tccacttgaa ttgacccatc ttcgtgtata actatagtaa
    16861 ctggctcttt tagaccttgg tatccttctg gtgtaaaagt ttcggtcagc gtataagttc
    16921 caggttttaa attttcgaat agaaaggtat ctgtttcttg tccatctttt ggcgattcta
    16981 agtccatttc tggtccctgc agtctgaatt ttgctccttt taatgtctgt ccgttgtctt
    17041 cttttttgtg taccgttaga tcaaacgctt ttagtcgatt ttgatgagtg agcgtccata
    17101 cattatttgt catctcggta ttttttcccg tgtaaatgat ttttccatat ttatcgattt
    17161 ggaattcata atttcccggc gaagtatcat atccggcagg accagctgtt tcaacgactc
    17221 gatatttacc aggtgtgagt gaatcaaaac tggcaagacc tttttcattc gttgtttggc
    17281 tgtcaattgg ctgataagta ccatttgctt cttttttctg tagggaaaat gctgctcctt
    17341 taagatttat ctctttgtcc gtcccttgca tcgcatactt acgtactgct acaggaactt
    17401 caacaaaagg attttcaata gtcaactgga tcgtatcatc ggaagtagtg actgtttttc
    17461 catcaatggt taccgtacca tcagaagcga tcttgatttc ccaagtagtc tttttgctta
    17521 atccatgccc ttctggagct tttgtttctg tcagcgtata ggtcatttct ttttgcaatt
    17581 tgacattttc tggaagagaa taggttccgt cgccatgatc tgttagtaaa gtatcaatag
    17641 aatctcctgt taatttgaaa acagcgccaa taagatcctt ttcacctgta gaggaatttt
    17701 tcgttatttt caagtttaac ggtgtgaatt gtttagtatt tttccatgtc attgcatcta
    17761 tttgttgaga atcgtatcct ggtacaggta attctttgat tgtttggtaa ctgaatgctt
    17821 gaccttgatt attgtattga ggtagtgata atatctcttg ataactttct ccgctatttg
    17881 cagataattt gtcaatgtct gcacgttccc acgtatttgt tgtatcttta gctggtttaa
    17941 tgattcgaat atatccgttt ttccaagctg cagcatttgt cgtatgttcc cgttgaatct
    18001 caaaagtaac ttgatctgga cgatcagcta gattgttgtc aaattcttcc cataactttt
    18061 tgatgtgaag actgactccg ggagctttag cagatggtat accgaattca gctaattcat
    18121 tggtatcaat gtttggctgg aaagttgtcc tgccgttcat ttgataccag aaatttggat
    18181 ggaagtcctc attttctgtt tggattctca cttggtaatg gatttggatt tcttggtctt
    18241 ttcctaaata gatctgattg ctcttgatgg tatttccttc tatggaaata gatggagtga
    18301 ccgttgtagg acttgtcccc acactcttga ctgaaagtgt accaggctga taaatgaatg
    18361 gttctgcgat tggatcattt atttgtccat tgcttacagt tgctgagatc tgtacagctt
    18421 ttttggctag gtattcagag atatctgttg catgatcagc tgattcatag taaagatcgc
    18481 ctttttcatc tgatgaaacc atttgtcgca tacgagactc tacttctgct tttgagagac
    18541 cagctgccgg atcgctttga agttggatgc caagaccatg tatttcgatt cctcgttctt
    18601 tgagtgccat cgcttctccg atggttgcga taaacgtact gtcgattctt ctggatagat
    18661 tgttttggtc aggtgcatca tagattcttg agattagaga agtatttccc ggccgatctt
    18721 gcgtattaga aaactgagtt ccgtaataat tgctgcttga ttgtgcgtgt acccgctgta
    18781 ctttatagga aaatgttggt acaccatccg tcagcaaaac gatcactttt ttatgtccat
    18841 ttggaacgga tagcatgctt cctgcatctc ttagtgcttt ttgtgtaaaa gtaccaccat
    18901 ttgtccgtga aggtgtaatg gattttactt gatttttcac tgaatcaaat gaacccatct
    18961 gtactgcacc gttactgtag ctatatcctt cgcttgagta gccgacatat cccatattga
    19021 ttttgtctgt gataccgcta tctgctaaag tatcgacaaa acgatcgaca ccaatcttta
    19081 cttcaccgat ccgattattg tcgttcatac ttcctgacca gtctacgacc aatacgagat
    19141 caagaggcgt gatatctttt tgtacatttc cacgagcatt caaataaaca tcaaacaatc
    19201 cgggtgtact tgtttccttt gcatactttc gtatcgcata gtctgcttga ttaccggttc
    19261 ctccatattc gatatacgaa tgggtccgat cattaggatc tccattccaa gaatttatac
    19321 catcccattg atttgttccg tttttattgc cttgatgatc aagaacattt gtatttcctg
    19381 taggttgcca actggcttta ggataagtgc cctgatcatc tgttgtatat tctgtaggaa
    19441 tcgtttgatt agatggcaca ctcacagctg gcaaggataa tgtaggcaat gtaaatgggc
    19501 tgacagaatt ttcgttttct tttgatgatt cttcttctat ttcgtctggc tttggtactg
    19561 acacatctgt atctttctta ttttctgtct catcaataat gtcatattct ttattatcga
    19621 cgatcagctt gtttgcctgg ctgtggtaaa gtgctggatc gattggaaaa ctcagatcga
    19681 tagtttgttt agtgtttgat gagatttcca acgtaatttt cccgctattt acctgataag
    19741 catagccatc tccttctttt ctttccacat cacttaagta attttcgact agaccaattt
    19801 ctttttctat cttttcagca ctggtgttat tcgcatttag cttgatgttc agtgcttttt
    19861 ttccagactg atccgaaatg acttcagctg aaattgtttc taaaactgtt ggatcatctg
    19921 tagtgtctgc tattgcagat aatggcacaa agtttgataa actcaatatt atcagcatga
    19981 aaataacttt cagtttcttc cctgtggtcg tcatttttcc cctccttttt ctttttttct
    20041 gttttgacgt gaatagtgaa aattattaaa ataaacaggc gttacataat tttatttatt
    20101 ttaatatgat aattattata cgaagggcgc taaagggaat cacaccaatt ttcataccaa
    20161 ttggtttcat ttatgaagtt gattccttcg attaatgagc gtttcattga aagaaaaggg
    20221 ttgagggaga aaaacatatg atcaggttgt tggaatcaaa ctatagaaag atggaaaaaa
    20281 tcattcgaat tctgtccatt tctgacaagt ggatgactaa aaaagaactt gccaaacgaa
    20341 ttggtagttc agaatctaca tttgtaaggt atatcgaaga gatcaaaatc cgctggggaa
    20401 cggatgtaac gattcaatct tcacaaaaat tggggtatcg tcttgaacat ttgaatgtac
    20461 cagcttattt aaatgttctg atggaaatgt cgcaaagttc tacaaacagt cgtttattaa
    20521 atgagattat tgtcaatccg ggtaaaacga ttgagtatta ttgtgaaaaa atttccatca
    20581 gccggtcttc ttttacacgt aagttgaaac aatgcaatca aattttagaa agctatttat
    20641 taaaagtgat cgttgaccaa ggttttcagt taactagcct taaaagtgag ctccagttgc
    20701 gtatctttgt tacctttttc tttttaagtt actacggaag acgcacgttg ccatttccaa
    20761 tcgaaaaaaa gaagatcaaa gaaattatgg tcaagaataa ttgtcagatg aatttgtttc
    20821 aggaagaaag cccatatgag caatctttct ttattatgta ttttgttgtg catctcatga
    20881 gagaatccca aggaactcca tttgaatgga aagctggaga ctcttatgta cagttgataa
    20941 atatcgataa aaaagacttc ttaatcttga aaaatctctt aggaaaattg aagtacagca
    21001 gctatataaa agtagtgaac tattttgtca atacgatttt ttcagaattt tcggagaaag
    21061 aaaaaaatga aatcagtgag cagatacgaa aaaatatgtc ctactgttcg ttttcatccg
    21121 ctgattgcaa agaaaaaaac gaagaatatg ttgttcaatt attaacgcaa atttattttt
    21181 tctcccgtat tttcccatac gatacgactc atttgacgat ccgtgcaact tactttgcac
    21241 agcagatgca acaccgccag atacgtctat atgctggatt tcgtgcagaa cttcaatttt
    21301 attccactct actgaatcag aatctgattt attatttatc tagctatatc ttttggatgc
    21361 ttatcggtaa tcctgaaatt caccattata cttctgataa aaaaatactg attatcagcg
    21421 acttaagtct tcgacatagt caatatattg aagaatatat cagtgatatt cttgctgtgc
    21481 ataaaattca ctctgagaca actgctatta ctgaagatca gctgtcgaaa tacaatcttc
    21541 tcgagtatga tctgatcgtt acgaatcaac ccattttgaa tgcccatgtt cctcatatat
    21601 tgattgatga tagtgtgtca ttcgcaaatg aagaagaact catccgctta ttcgagttat
    21661 gaataatcaa aaaacagcta tgtataaacc accctgataa aaatttcagg acaaatacac
    21721 atagctgttt ctttttattg tattccttca gcaatccaac gagatttata gcgaaagtct
    21781 tttaacaact cttcttttgt ccaattattt gcaatgagat ccgtcaaagt gttcattacg
    21841 agggtcatgg aaaagtatag gaaactaact gcttcttccg acgattgaat ggagaaatga
    21901 ttgattctta ataattccag ccatttttcg ttcattggag aatcagccga tagttctcta
    21961 cgtttggttg tctgctgatc atttcgctgg gttaacaagt ataacctttg ccaatagata
    22021 ctttcgtgat ctaagtcgca gcaccactct aaatattttt cgatccccaa aaagaaatgt
    22081 tgtttgtttt cgctaatgaa ttttaagata tcttgatgga ctatgtgaga actgcttttg
    22141 atcgtataca aataagcatc ttccaaatct tgaaaatatt tataaaaagc accgcgcgac
    22201 atccccatct tgtcaacgat ttctgagact ttgacttggc tgatgtgctg tgagcaaaat
    22261 atctccagta ataggtcatc gattctctgt ttctttgcgt tcgataaatt gaaaaaagtt
    22321 tcttttggca tccacatctc tcctttatct ctgattatac gtggatagcc cttcattgtc
    22381 atgaaggatt cattccttct ttttctcttc ttctgtcaag tacccatctt ccattcgata
    22441 aacgcgatca ctccattgaa tcatacggac atcatgagta accataatag tcgctttttg
    22501 tttctcatga gcttctttaa ctagtaattt gacgacttca tatgcatgat ctgtatccaa
    22561 actagctgtt ggttcgtctg ctaagatcaa gcttggctca ttatataaag cgcgagcaat
    22621 tgccactctc tgtctttctc caccagaaag gtctttagga taactatttt taagtgactg
    22681 gatatctaaa gattgcagca aggaatcgat ttttgttgaa tcttcttttt ttgaagtaac
    22741 ttggtcaact agttcaaatt gttcctttac cgttaaaaaa ggaatcaaat tagagctttg
    22801 gagaataaat cctatttctt taaagcgtaa atctgctcgt tttttttctg gaagctcagt
    22861 tagtgagtga ccgttgatca ctatttctcc ggaagtcggt gtttgcaatc ctcctgaaat
    22921 ggtaaggaaa gtacttttac ctgaaccaga aggaccaatg atgctgacga attctccttc
    22981 ctttacataa aaatcaattg ctttcaaagc tgagaccttt gtatgaccag aaccaaatgt
    23041 tttggtaact tttttcatcg ttaatatttt tttcattttt attaacctcc tattgctgta
    23101 attgggtcta ctttggttat cgtacgtatc gaaaacaatc cgcctagcat tgcgacaatg
    23161 atcaaaatcc cactatatag caaccattgt gaccaaaaaa cagcaaaagg catcgcgctt
    23221 ggtaaaatca gactagtcaa ataagcaaac aaaagagcaa gcagaacgcc taccacccca
    23281 acaataaagg actgtgctac taaagattta gcgatgaaga aatttttgat cccttgtgct
    23341 ttcattactc caaaaatagc agtcttctga agtgtcatca cgtacatgaa aataccgacc
    23401 acggcagcaa ccactaagaa caaaaagtat atcatcgcat ttaaggtcat attttgggca
    23461 gaatagccag gaatactttc aatgaatgtc ccaatagtta ataactgtag acccttcgat
    23521 gctttttctt tcgaaatttt agctgaatcc tttattacga tcccattgat cggctgttct
    23581 tcttcagaag caaacggctg ttgtccatac ttcaaagcag tccacgtatc tagggacgca
    23641 tagatgacgg gagaaactgt gtaataagtt tcaggaaaga taccgacaat cgtcaaagga
    23701 tcgtcgtaac tgccaatttt gatcgtatct ccaattttat agcctgaatc agccagattt
    23761 tgagagatga tgatttcatt ctttgtttta aattcatgcc ctttcgtgag ttttggtaat
    23821 aaaaatgctt ctttcgttgt gccgaaaact gtaacatttt ctttgttttt tcctttgatt
    23881 gctccgctgt acaatccgat tggtgctttc tctcctcctt caatgtattt cagatctcct
    23941 cttgttaatt gtgaagctgc aaatacttga tttgcatcct ccgacaaaac aatctcttgt
    24001 gcgtcccaat catcgattgc ttttttaaac tcttctgaaa gtccattcgc tagtcctgac
    24061 aacatgaata cgacatacgc aatcaagaac ataataccga cgatcaagcc atatcgtaat
    24121 ttagaatagc gaatttcttt gatagctaga aacatttgtt tttcctccct atgatagatt
    24181 gatagtgaca aaatgtcact tgttgaatag tgtaactcta aagtgacaat ttgtcacttg
    24241 tgttgttttt ttcttatcaa taaattgtct aagacatctt tcgataaaac cttaacatga
    24301 gtcataaaaa atttatttaa gccttattta tgctttccat gataaactaa agtgaccaaa
    24361 tcaatgaggt gggaaaatga ttaaactatt agtagtagaa gacgataaag tattatccaa
    24421 taatataaaa gaaatactcg ctgcaatcgg cgagataaca caagtttatg atggtagtga
    24481 aggactgttc gagttggaaa gtggagttta tgatcttgca atcttggatt tgatgatgcc
    24541 ggaaatgaat ggatatgaag tgttagcaaa aatacggaaa gaaaaaatac agactccagt
    24601 attgattttg actgcaaaag atagtttgga agataagatt gaaggatttg aacgaggtgc
    24661 agatgattat ctaacaaaac ctttctatcg agaagaactg atcatgcgcg tgaaagcatt
    24721 attgaaacgc tcgctaggtc tctttgatga aaatgttctg aagtataagg atatcagttg
    24781 tgatttaaaa agcaatgaag tcttgtataa agaacaagtt ttgcccatac aaggaaaaga
    24841 gtttgaattg ctcgtttatt ttttacaaaa taagggaatc attttgacaa aagagcagat
    24901 atttgaccgg atctggggat ttgattctga tacgacgatt acagttattg aagtttatat
    24961 gagcaactta cgaaaacatc tgcgaagtac tggattggat aaagaaatca aaaccttacg
    25021 taatgtcggt tatatactgc aaagggtgtg atgcaatgaa tggagatatt acacgaaagc
    25081 aacgtagtcg cttttttgcg acaaatgtag cagcatttgc ttttatcttt tttcttttgg
    25141 gattcattac tttacagata ttaaaccaat ctgcatacag agaaacagat ctgtcattag
    25201 agaaaataaa ttttgattct cgaacagtac aattagagat acaaagatat cagcaaggcg
    25261 atcccttttt aaacccgact caaacggaac aaaacagaac aaacatggaa cagccattaa
    25321 tggaaccatc aaatgaccgt tttaacactc aagtgatctt gtggtctaaa aatggtgaga
    25381 tattgaataa agcagctatt ggtgggcgtt tcaaccaaat caaagaatta aaaatggata
    25441 cgaaaaatct gaataccatc caaaatattg aattaaatga agacagtggc tcatctgccc
    25501 tctctttccg ttcgattacg aaaaaagcgg agacgaatca agatggcgtt gcctacatcc
    25561 aaattttaga aaatacaaat caaatcaaga attcattagc gacttttcgc acgattatga
    25621 ttttgtgtat gattgttttt tggattctct ctattggtat cagttattac ttgtctagtc
    25681 taagcatgcg ccctattatt ctttcttgga gaagacaaaa agaatttgtt gagaatgctt
    25741 ctcatgaatt gcgcacaccg ttaacgatta ttcaaaatag tttggaacat ctttttacca
    25801 agcctaatca tactattatt gaagaatccg aaagcatcgc tcaagcattg tcagagacga
    25861 ggcgcctcac tggattgact tcggatttgc tgacaatcgc tcggaatgat tctgatcagc
    25921 agctgctttc aaagcaaatg atcaatacac aagaatatat cgaacaactt gttaaaccat
    25981 ttcaagagat ggccatcata gatggaaaag agtttattct ccataacgat gcaacattag
    26041 aagtaccaat tgatgaaaaa aaaatccatc aagttttggt tattttgctg gataatgcat
    26101 taaaatatac gaatccagaa gataagatca ttttggattc atctgttaac cataaagaat
    26161 ggctgattca cgtaaagaat actggccctt ctatcagtga agaagatcaa gcaaggattt
    26221 ttgatcgatt ttatcgggaa gatcaatctc gggcgaaaga gactggcgga tacgggcttg
    26281 gcttggcaat tgcaaaacag gtaatcgaac agcatcatgg acagatttct gtatccaatt
    26341 atcttccaca aggagtcgat tttaaagtcc aattgccgtt gaagcctaaa aaataatgct
    26401 tatccctaaa aaagggctgt gacaaaaatc ttgtcacagc ccttttttgt tgcgaataaa
    26461 cggtgttcca gaagtagctt cttcggaaat aagccgaaat ttctttaagc tgaccacttc
    26521 tgtcacaacc tctttagctt acgcttacga ttttttattg cggtcttcaa tgtgtacatc
    26581 accgcttgtg tcaatatcca attcttctcg gctaacatct tctgtcactt tttcaacatc
    26641 ttcatgttct tctttatgaa tcccaacttc ttcacgaata actgtatgtt tgcttacatc
    26701 tacttgctct tctttgattg ggatagtaat cgtgtcatct tccaattttt cgtctgttcc
    26761 ctgtgaagat tgatctgtta ctggtttgcg ttcgatgacg atttcttcac gtttgaccgg
    26821 tacttctacc gtttcagtat cgttgaccac atgtttgtgg atgtcgactt ctccagttgt
    26881 cacatcatgt gtatttacat ctaatttttc ttcttttagt tgaattgtat catcatctag
    26941 atttggtttg cgatcgatgt cttcagtctt gcttgtaccc attgtatcag tctcgccagg
    27001 tacgtccatc ggatcagttc ctggtgtcat attcaatgga tccgttgtat tttgaaagcc
    27061 tgtcgtgcca cctacgtcca ttggatctgt tccagtgcca gtttctaatc cagtccccat
    27121 tgtgcttgct gggctaacag tatcttgtgt atccattgta tcttctgttg tttctggctg
    27181 acgatagcct tttactgtga tgacaaattt tccatcagaa atatctcctc tatattgata
    27241 taaaggatca ttttctggtg tagccgtctc agaatcatag gtatcaaaag aaaaagtatc
    27301 ttttattttt tcccataaag aacggtcttc ttcaccatcc atccgatttt catctgtttc
    27361 aacatctata ccagacaatc cgtcaaatcc tagtctagat gctgcttcat cagtagtata
    27421 aataacgata tcatctcgtt catacccttc gttacgcaac cgttcaatta ctgataatgc
    27481 ttcttccctc gtactatagc ttccgatgac cgttaaattt ttctcttcca tgtaaattcc
    27541 tcctagcaaa tagatttatg attggtatat tgttacttat aaatttattc tataggagac
    27601 gattgttttc tacaaatatt tagattggaa tttgattaaa acaatttaat tgaaatttga
    27661 aaacatttat gttaatgaaa gtcatattct aataaaatgt catttgtttc tatatgaatt
    27721 tttatgaaaa atttcatata atttccactc gttgtgatgg tttaatttat ccaatccatc
    27781 aataagcaaa caacttatat aaaaaaggat taattctcaa tataattgct cctaccatag
    27841 aataaatctt catgtaaagt aatgaagtac caactatttc taatacttct ttaagaatta
    27901 tcccaacaat atcgttagac aaaccattat tgaataaatg gagagccaat cgattctgaa
    27961 aaaatgtttt ctaatttttc ggaaataaaa gaaagagcct cctgccatta gaacgaacca
    28021 aatccatttc attgctcatg aagtaaccac atagctaaaa agaggataaa tcaattaaaa
    28081 gatcaaaaga atgactgcac taataaaata actaaaaatg tatcttctta cttcatatct
    28141 tttagctagt acttagttaa aaatttgctt cctaagatca agacaaactg tctatcttca
    28201 tttgatgaaa tgcggtaaaa tatagaaaat gaacactatt ggagggaatt tgtaatggac
    28261 agcaaaacta gaaaacaact gcaagaaaag ttaaagcaac gtacacactt gctcccttcc
    28321 gtctttcaac agtttttaga agaaaatcgt tcttcccttt cattaggcag tcgctatgaa
    28381 tacgcaaaag attttcatct gtttactgat tatttgagaa aagtattttc tgaagaagtg
    28441 gatgatcagc tgattatcca tttagaaaaa caagattatc agaatttctt agctaaaatc
    28501 tatcgacata ctcgttcgtt ccaaactaca gcgaaccaag aaacggagca attgtttgaa
    28561 aatagctatt acgggatttc aaggaagcaa tattcattaa atcatttttt acgatttttg
    28621 tatcaaaaag gactgattga agaagagatt tctcttttgg gtacaagttt accggaaaca
    28681 accaaagcag ctccgcgata tatcgatgca gaacttttca aagattttga gcatttgttt
    28741 atcccgattg aaggctttca aacaagcaga gaagctagct ttgaagaaaa gaatagaccg
    28801 aggaaccttg caattactgc cattcttctg tattgcggat taaagatcca tgaagtagta
    28861 gaacttaaac gtaaagatgt atctttgacc aagcagattc ttaatctgaa tagaaaagaa
    28921 aacagactga ataaagtacc aataccaaaa gaagcgatgc catttctgga gaactatttt
    28981 aagttaacag cacaaaatac ggacgaaaat tcttttgttt ttcgttcctc acatgatcag
    29041 caaatttctc ctcggactat tcgccaaatt ttgagcaaaa ttacagtata taaaaacgta
    29101 tcgccagaac tatgcagaaa aacatatcgt tattatgcag aactatattt agatgatgct
    29161 gacgcggtaa attatttatg tggtaatcga tacctctcat cccattcttt tcaagatatc
    29221 tgggaaaaga tggttgattt ttcttatcac gaattagcgg cattcgttcg ggtttcttcc
    29281 atataaaaca aatgtatgat ctttttttca caataaacta tgttattata taaaggatta
    29341 tgttacgatt atcttgaatt tgtatattac ttaacaaaag ataatggagt gtgaatgatg
    29401 gagaccataa cgtattcccc tttgaactta tttacgaatt ttcaagaagc tgcagctaac
    29461 ttttccgaga ccccgattat ctttgatgag ccactttcag ctttcccaga attaggactt
    29521 gaaacaactt acaaaaaatg ctgcgaaagt gtgttgaaac gggcttatca attggctcat
    29581 cttggagtga aatccggaga caaagttatt atttataaga gttccgcttt tgacacatac
    29641 cttctagctg tttctgtgtc ttaccttggt gcagtgcctg tcatgacttc ttatcatcta
    29701 ccaacaacaa caatggaagt atttatcgat cgtttagaag accctttcat tttatttgat
    29761 gatgagacaa aaaagcgagt gagagaaatt tcaaatggaa caaaatcaaa acagattcc
//
LOCUS       DACDKM010000021        29583 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20834_2016
            SAMN12640370-rid9891663.denovo.021, whole genome shotgun sequence.
ACCESSION   DACDKM010000021 DACDKM010000000
VERSION     DACDKM010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640370
            Sequence Read Archive: SRR10040891
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 29583)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29583)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742835.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742835.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:04:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,012
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 29 of 75
            Pseudo Genes (incomplete)         :: 33 of 75
            Pseudo Genes (internal stop)      :: 19 of 75
            Pseudo Genes (multiple problems)  :: 5 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29583
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640370-rid9891663.denovo.021"
                     /isolate="E20834_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            375..1484
                     /gene="pdhA"
                     /locus_tag="IV334_000963"
     CDS             375..1484
                     /gene="pdhA"
                     /locus_tag="IV334_000963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring) E1
                     component subunit alpha"
                     /protein_id="HAP8943053.1"
                     /translation="MANKKAPIDFQALLDEVNADFPVYQALDQDGNVVNKDLVPDLSD
                     DELVELMTHMVWSRVLDQRSTALNRQGRLGFFAPTAGQEASQLASAYAFDKEDVLLPG
                     YRDVPQLVKHGLPLSQAFLWSRGHAAGNSYPEELKALPPQIIIGAQYVQAAGVALGLK
                     KRNKKNVVFTYTGDGGSSQGDFYEAINFAGAYHANAVFYIQNNGYAISTPREKQTAAK
                     TLAQKAVAAGIPGIQVDGMDPLAVYTVSKMAREWAISGNGPVLIETLTYRYGPHTLSG
                     DDPTRYRTKDTDDDWQKKDPLIRFRKYLTEKGLWSEEKEEQVIEATKEEIKAAIAEAD
                     KVPKQKVSDFLKNMFEVSPQSIKEQIAIYEAKESK"
     gene            1487..2464
                     /locus_tag="IV334_000964"
     CDS             1487..2464
                     /locus_tag="IV334_000964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HAP8943054.1"
                     /translation="MAQKTMIQAITDALALELENDENVVVFGEDVGKNGGVFRATEGL
                     QEKFGEDRVFDTPLAESGIAGLSFGLALEGFRPVPEIQFFGFIFEAMDEVVAQMARTR
                     YRMSGTRNLPITIRSPFGGGVHTPELHSDNLEGLIAQSPGIRVVIPSNPYDAKGLLIS
                     SIRSNDPVVFLEHMKLYRSFREEVPDEAYEVPLDKAAVTREGTDVSIITYGAMVREAI
                     KAADNLAKENISVEIIDLRTVAPLDVETIIQSVEKTGRVVVVQEAQRQAGVAAQVVSE
                     ISERAILSLEAPIGRVSAPDTVFPFGQAENVWLPNAKDIEDKVKEIAEF"
     gene            2489..4132
                     /locus_tag="IV334_000965"
     CDS             2489..4132
                     /locus_tag="IV334_000965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005236379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyllysine-residue acetyltransferase"
                     /protein_id="HAP8943055.1"
                     /translation="MAYQFKLPDIGEGIAEGEIVKWFVKPGDTINEDDTLLEVQNDKS
                     VEEIPSPVTGTVKNVIVPEGTVANVGDVLVEIDAPGHEDNEGDSGVAAESQTPAKPAA
                     EPTVDTESAGSSSEGVFQFKLPDIGEGIAEGEIVKWFVKPGDTINEDDTLLEVQNDKS
                     VEEIPSPVTGTVKNVIVPEGTVANVGDVLVEIDAPGHNSAPSTSAPSAEAPKEKVETS
                     GSASVVEAADPNKRVLAMPSVRQFAREKDVDISQVTATGKGGRVTKEDIENFLAGGPS
                     SAPAKSEAPEAAAPKEAAPAAESKPAAPAKPFKSNLGDLEERVAMTPTRKAIAKAMVN
                     SKHTAPHVTLHDEVEVSKLWDNRKRFKEVAAANGTKLTFLPYVVKALTATVKKYPVLN
                     ASIDDANQEIVYKHYYNIGIATDTDHGLYVPNVKDADRKGMFAIADEINEKAKLAHDG
                     KLSAEDMRNGTITISNIGSVGGGWFTPVINYPEVAILGVGTIAQQPIVNAEGEIVVGR
                     VMKLSLSFDHRIVDGATAQQAMNNIKRLLADPELLMMEG"
     gene            4140..5546
                     /gene="lpdA"
                     /locus_tag="IV334_000966"
     CDS             4140..5546
                     /gene="lpdA"
                     /locus_tag="IV334_000966"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HAP8943056.1"
                     /translation="MVVGDFAVELDTVVIGAGPGGYVAAIRAAEMGQKVAIIEREYIG
                     GVCLNVGCIPSKALIAAGHHYQESLDSEMFGVTSENVKLDFAKTQEWKENKVVHTLTS
                     GVGFLLKKHKVETIEGEAFFVDDHTLRVIHPDSAQTYSFNNAIIATGSRPIEIPGFKF
                     GGRVLDSTGGLALKEVPKKFVIIGGGVIGAELGGAYANLGAEVTILEGSPQILPTYEK
                     DLVKLVEDDFKKKGVTVVTNAMAKEAVDNGDSVTVKYAVDGKEESVTADYVMVTVGRR
                     PNTDDMGLEQAGVEVGERGLITVDKQGRTNVPNIYAIGDIVPGAALAHKASYEAKIAA
                     EAISGKKVAVDYKAMPAVAFTDPELASVGMTIKEAKDAGIEAKAYKFPFSGNGRALSL
                     GKTEGFIRLVTTIEDNVLIGAQIGGVGASDMVSELALAIESGMNAEDIALTIHPHPSL
                     GEIVMDASELALGLPIHI"
     gene            5708..5983
                     /locus_tag="IV334_000967"
     CDS             5708..5983
                     /locus_tag="IV334_000967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0223 family protein"
                     /protein_id="HAP8943057.1"
                     /translation="MQEYQYPLDLDWTTDEMVIVMKMWEDLERANEKGINNEAFLKTY
                     QQFKTVVKSIGEEKRLGREFEKASGYSLYRTVQQAKKNPNKTLRMNG"
     gene            5989..6756
                     /locus_tag="IV334_000968"
     CDS             5989..6756
                     /locus_tag="IV334_000968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HAP8943058.1"
                     /translation="MEQMIDHIKSWLEEAGEKIKSSQQTLTISQKSNRTDLVTNVDRN
                     IQNFLIEKIHSFDPEAKILAEEEGCNQLSDLDGRVFIIDPIDGTLNFVIEGENFCIML
                     AVYESGVGKLGFIYDVMRNELFWGGKGIGVYKNDEPLPRPANKSLSEGLLGVNSYLFG
                     HNKYGIREIAESSMGTRMYGCAGLELVAILKGNHIAYISNLSPWDYAAGNVLLEEFGM
                     VTSGLFGTPLTFEKREYYLAGTSCAYAEIADLLKKEG"
     gene            6924..8753
                     /gene="typA"
                     /locus_tag="IV334_000969"
     CDS             6924..8753
                     /gene="typA"
                     /locus_tag="IV334_000969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HAP8943059.1"
                     /translation="MNYRNDIRNVAIIAHVDHGKTTLVDELLKQSDTLDAHTQLQERA
                     MDSNALEKERGITILAKNTAVDYKGIRVNIMDTPGHADFGGEVERIMKMVDGVVLVVD
                     AYEGTMPQTRFVLKKALEQHITPIVVVNKIDKPSARPEHVVDEVLELFIELGADDDQL
                     DFPVIYASALNGTSSLSDDPADQEPTMAPIFDTIIEKIPAPVDNSDEPLQFQVSLLDY
                     NDYVGRIGIGRVFRGTIKVGDQVALIKLDGTVKKFRVTKLFGFFGLKRLEIQEAKAGD
                     LIAVSGMEDIFVGETVTPVDHQDALPILHIDEPTLQMTFLVNNSPFAGREGKFVTARK
                     IEERLMAELQTDVSLRVEPTNSPDAWTVSGRGELHLSILIENMRREGYELQVSRPEVI
                     EKEIDGVKCEPFERVQIDTPEEYMGSVIESLSLRKGEMQDMVHTGNGQIRLTFLTPAR
                     GLIGYSTEFLSMTRGYGIMNHTFDQYLPMLPGQIGGRHQGALVSIDTGKATTYSIMSI
                     EERGTVFVEPGTEVYEGMIIGENSRDNDLTVNITKAKQMTNVRSATKDQTSVIKKPKQ
                     LTLEESLEFLNDDEYCEVTPESIRLRKQILEKNAREKASKKKK"
     gene            9014..10786
                     /gene="recQ"
                     /locus_tag="IV334_000970"
     CDS             9014..10786
                     /gene="recQ"
                     /locus_tag="IV334_000970"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase RecQ"
                     /protein_id="HAP8943060.1"
                     /translation="MNDLLKQYFGYDEFRPGQKEIIQKVIDQENVLGIMPTGSGKSIC
                     YQLPALLLDGLTVVVSPLISLMKDQVDAANQLGIPATFINSSLDGYETARRFQEIDRQ
                     QYRLLYIAPERFIMPDFIQAMKRWNVRMIAIDEAHCISQWGHDFRPSYLQMANQLDQL
                     PNRPVIVALTATATVQVAADIKRLLKIPENNHIQTGFERENLRFQVIKDQKKEQYLIE
                     YLKINKNQSGIIYAATRKEVDRLYHLLKKFDFSVGRYHGGLNENERTEMQEAFLYDRL
                     QLIVATNAFGMGINKSNVRFVIHYQIPGSLEAYYQEAGRAGRDGLSSEAILLFSPQDI
                     QVQKFFIQQSQREEGQKQKEYEKLKAMTEYVYIESCLQQYILNYFGETSSPCNRCGNC
                     LDDREIVEVTTEAQMVLSCLKRMGENYGKQMLMKVLAGSKEQKLQALGFGHLSTYGLM
                     KNQSQKETMQLIEYLISNGYLLTINGEYPVLKVTERGIQVLKGQESVYRKEPKKVQQL
                     SDEETDTLFEVLRELRTDLASEAGVPPYVVFSDSTLKEMSRIRPSSRLEMLQIKGVGQ
                     SKLDKYGEAFLSRIKNADSNVLDK"
     gene            10900..11202
                     /locus_tag="IV334_000971"
     CDS             10900..11202
                     /locus_tag="IV334_000971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlaN family protein"
                     /protein_id="HAP8943061.1"
                     /translation="MDSLSKKLAIQVLNDEAERIKRLIRNQNNSLCISQCKAFEEVVD
                     TQMYGFSRQVGFAVRIGVIGKNEGQLMLSELERELNQLYSEVYQEIYDKKEVGKEE"
     gene            11205..12368
                     /locus_tag="IV334_000972"
     CDS             11205..12368
                     /locus_tag="IV334_000972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HAP8943062.1"
                     /translation="MPKKVKKRHLLDYSILIPYLILCVLGLIMVYSSTSYLLLENGQN
                     PSASVINQSIFWVLSLIAIALLYKMKTDVLKNQRLIMAAIAVLTILLLIVVFFGKEIN
                     GAKGWLQIAGFSIQPAEYLKIISIWYLSLTLSKRQNSVQKDFLGTVKRPLAMVIGLTA
                     LVAILPDFGNAAVIFLIILVLLLASGVNYVYTLIVGVGGFCLSTFTIWLINITNGKIL
                     PGRLQYIYNRFAIYQNPFSDELNKGHQLVNGYYAMFNGGLFGRGLGNSIQKKGFLQEA
                     QTDFIYAIVVEELGVIMGILILALLFFMIVRIVLVGIRSKDPFNSLLCIGIGAMFLIQ
                     VFVNLGGITGVIPLTGITFPFLSQGGSSLLMLSICVGFVLNISADEKRKSLGL"
     gene            12394..15822
                     /locus_tag="IV334_000973"
     CDS             12394..15822
                     /locus_tag="IV334_000973"
                     /EC_number="6.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate carboxylase"
                     /protein_id="HAP8943063.1"
                     /translation="MKKVLVANRGEIAVRVFRACTELGIKTVGIYAEEDEYSVHRFKA
                     DEAYLVGQGKKPIDAYLDIEGIISIAKECGADAIHPGYGLLSENLNFAQRCEEEGIIF
                     VGPKLHHLDIFGDKIKAKAAAIEAGIASIPGTDGPIASIDDALEFAKQYGYPIMIKAA
                     LGGGGRGMRVAHDEKSAREGYERAKSEAKAAFGSDEVYVEKYIANPKHIEVQILGDTH
                     GNVIHLFERDCSVQRRHQKVVEVAPCVSMNEQQRQKICQAAVQLMKYVGYVNAGTVEF
                     LVEGDDFYFIEVNPRVQVEHTITEMITDIDIVTTQLLIAQGLDLHKEIGLPQQEGIKL
                     NGSAIQCRITTEDPLNNFLPDTGKIDTYRSPGGFGVRLDVGNAYAGYVVTPYFDSLLV
                     KVCTHGATFETAIQKMERCLKEFRIRGVKTNIPFMLNVITHPEFQSGNAKTTFIDSTA
                     TLFEFPRLRDRGNKTMKYIGEITVNGFPGIESGEKPFYEEPRMPKDLITRTDYVTAKN
                     VLDAKGADALVEWIKGQENLLLTDTTFRDAHQSLLATRVRTKDFKQIARLTGEGLPEL
                     FSSEMWGGATFDVAYRFLNEDPWQRLRKIRSLMPNTLLQMLFRGSNAVGYSNYPDNVL
                     VEFVKEAAAQGIDVFRIFDSLNWTPQMEKSIQAVRDTGKIAEAAICYTGDINDPSRAK
                     YNVQYYKDMAKELEQLGAHIIAIKDMAGLLKPQAAYRLISELKETTDLPIHLHTHDTS
                     GNGIITYSAASKAGVDIVDVAMSAMSGNTSQPSMSSLYYALVNGPRLPEITIENAQKL
                     NHYWEDVRMYYKPFENGLNAPETEVYMHEMPGGQYSNLQQQAKAVGLGHRWDEIKQMY
                     HTVNLMFGDIVKVTPSSKVVGDMALFMVQNDLTEEDIYEKGETLSFPESVVTFFQGEL
                     GQPVGGFPKKLQKIILKGRPALNERPGLLAESVDFNEVKKELAEKIGYEPKQDEVLSY
                     LMYPQVFLDYQKAYNQFGDVTLLDTPTFFQGIRLGETINVQIERGKTLIIRLDEIGEP
                     DIEGNRVLFFNLNGQRREITIKDNSIISTVQTRLKAEPTNREQIGATMSGSVLDVLVK
                     KGDNVKKGDTLMITEAMKMETAIEARFDGEVAHVYVSSGDTISSGDLLIEVTEK"
     gene            15843..16967
                     /locus_tag="IV334_000974"
     CDS             15843..16967
                     /locus_tag="IV334_000974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8943064.1"
                     /translation="MKRLGLFWLILLLVIVAFYMEPVLFPPKLEVKENSDQQISNQMT
                     TWKYQELKATGFSTYIGKPIDQLEKDFGQAYDRLTSGFGFETRYYENKASHLTFEANI
                     QDSRVTTVKVLQTGPADIAPFYLGMTMQDLTKITTIYTNFTFEYHDTEVGIELMEEDM
                     NYRPLIAFDNDTFAILFFDQTTNGLFSVVYLDKDSLLKLLPYQVFGEGLPHYQADKSA
                     DWESINRVKERKSTTLLNMLRREDELPIYNQTLAFQNHSQLLLHDFLTRPEEILSEER
                     MADWQKSLGSAQTTVKFSLTNDELEKLISKQKLENTSGLFIHPAIDATFSFLYWYSDP
                     YNHDRFMDPGTDSVGIAFSKENMLVLLQEENGESSDSSDR"
     gene            16957..17394
                     /locus_tag="IV334_000975"
     CDS             16957..17394
                     /locus_tag="IV334_000975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HAP8943065.1"
                     /translation="MIVNEELFQLEDQCLQVAEMIKKSKTMASYLEHKQSMEESAKVC
                     QLKEAFLSKKEAFENVAAYGEYAPDYREKKRAVRKAKRQLDMDEHVAAFRYSETQLQS
                     LLDQIVQVLAEEVSNEIKVDAGSPFFETKKHFGCGGNCHAIRQ"
     gene            17378..17716
                     /locus_tag="IV334_000976"
     CDS             17378..17716
                     /locus_tag="IV334_000976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbG family protein"
                     /protein_id="HAP8943066.1"
                     /translation="MQLDNSEFEITKRRGLIVWVYSLKQLKNLKKFGLLHYVSRRMKY
                     VVLYLNEETLEETEERIRKLHFVRNVERSYRPDIEMNFAEKIGNKTVLKDEGFEIEEL
                     NTEIRLAEHL"
     gene            17835..18395
                     /gene="rsmD"
                     /locus_tag="IV334_000977"
     CDS             17835..18395
                     /gene="rsmD"
                     /locus_tag="IV334_000977"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HAP8943067.1"
                     /translation="MRIISGNYGGRRLKSLAGANTRPTTDKVKESIFNMIGPYFDGET
                     VLDLFAGSGGLAIEAVSRGCSHAVCVDKNYQALKIIKENIEITKEPEKFTTIKLDADK
                     AIERLAAEKQQFDYLFLDPPYAKQKIIDQIHKMEELGLFSKTAVIVCETDKTVELPET
                     VAGFHQIRKQIYGITAVTIYRKEGNQ"
     gene            18392..18883
                     /gene="coaD"
                     /locus_tag="IV334_000978"
     CDS             18392..18883
                     /gene="coaD"
                     /locus_tag="IV334_000978"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HAP8943068.1"
                     /translation="MKRALFPGSFDPFTKGHLDTVERAAKLFDEVVIGVFINTSKKSL
                     FPPEERMTLITKAVSHLPNVKVMHQENQLTVETAKEIGADALIRGIRSIKDFEYEREI
                     AQMNHHLYDELETVFLLAKPEYSHVSSSILKEVLHFGGDVSSYLPPVINEALAGKRES
                     NES"
     gene            18873..19916
                     /locus_tag="IV334_000979"
     CDS             18873..19916
                     /locus_tag="IV334_000979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HAP8943069.1"
                     /translation="MKAKQWLKTVLIIIFGMLIIGFVVVPLPYYVESPGATIDLKELI
                     TVNDKEDKGSGSFSLTSVGIRQATGFLALKAKMTPFEELISEEELTGGATNEEYNQMQ
                     QYYMDSSQNAAIEQALKLADKPYKMEFKGVYVMSVEKTSNFFGKLSVGDTVTKVDGKS
                     FQSTEAFMDYVKSQKVGQTIEISYLHNGEEKTASGDLIELPTDKKAGIGITLTDHTEI
                     ESDTDVRINSGSIGGPSAGLMFTLEIYEQVTGKNLRHGKQIAGTGTINSEGEVGRIGG
                     IDKKVASADKAGVEIFFAPDDTISDDVKKEYPEIKTNYEEAKAAAKKLNSSMKIVPVK
                     TVQEALDYLEKMK"
     gene            20075..20803
                     /locus_tag="IV334_000980"
     CDS             20075..20803
                     /locus_tag="IV334_000980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016925838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein ComEA"
                     /protein_id="HAP8943070.1"
                     /translation="MKGVMNDFYWSFFEKRTMIEKISKKILASPKLIIWMSMIVLIFC
                     IASGFLLSNLLFYRETEETAAKEILSSFSSSGEDPSAEGNDVEEDARPKVMYTDIKGS
                     VKEPGIYSFSSEERVYDVLKRAGGLLEEADSDRINFSAKIEDQQVLYIPAVGEELPEH
                     LDQLASSEGKQPTADSEPPKININTATPSELQQIPGIGSVKAQEIIRFREENGSFQKV
                     EDLQEISGIGEKTVEKLKNFVTIK"
     gene            20919..21164
                     /locus_tag="IV334_000981"
     CDS             20919..21164
                     /locus_tag="IV334_000981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HAP8943071.1"
                     /translation="MIVRKETLKKPMLNVYLQNKISGIHIMNTAVSGNNSQALRERFA
                     KDVLSYTADKVFILIGTNDLAEHKQLSKETYQKICSG"
     gene            21161..21379
                     /locus_tag="IV334_000982"
     CDS             21161..21379
                     /locus_tag="IV334_000982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HAP8943072.1"
                     /translation="MIVQLKKDYSSDHAFFITPPAVDETKQKYRNNKLIKEYISVLTE
                     VAKRHDCEVSIFIRNFLNKWIRRIFIMG"
     gene            21515..22054
                     /locus_tag="IV334_000983"
     CDS             21515..22054
                     /locus_tag="IV334_000983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComE operon protein 2"
                     /protein_id="HAP8943073.1"
                     /translation="MLEKSSKACDTNKEKVTDKSHNERIPWDQYFMAQAVLLSLRSTC
                     TRLEVGATLVREKRIIAGGYNGAVSGDVHCIDEGCYVVDGHCLRTIHAEMNALLQCAK
                     LGIPTEGSEIYVTHFPCLACTKALLQAGVKGINYLHDYRNDPYAQALIEQLGVSVHQV
                     ALDSQHFSKLQNELTGKHV"
     gene            22047..24320
                     /locus_tag="IV334_000984"
     CDS             22047..24320
                     /locus_tag="IV334_000984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA internalization-related competence protein
                     ComEC/Rec2"
                     /protein_id="HAP8943074.1"
                     /translation="MSSVLERHVRNRLIFPAITSMIICLLYYTDNWGYRLLLAGIFLW
                     FSLKEGRFIAYLCILCSITVSISYRLQEYSFLEEKTTSAQITVFTDTIRANGNFLTME
                     GRLSENKQKLQLSYQVNTEAELKTWLNFRGKGIELTAEGEFISPAKKRNLYNFDQEAY
                     FRNHRISGRFQIQKIEQITMQNHWRNWLQRKRGSFISFVQSEFPQKTSVYINSLLWGY
                     KDAGFREPEEIFRESGLLHLFSLSGLHIQFYLGWLYYLFRRIGWPLHVSIIPLSLLTI
                     CYVSIAGSSISVLRASLLFLLRLLVKLLNKHYSTMDLFAVVFWLLLLFDPRFLFHAGG
                     QLSFFMSFLFILISFDGDWLKKTASHVLFTMITMPLIVWHFYEWPILGSLFTLLIAPV
                     FKFLFLPGVLFLTLFNRWLPETLLLLIEEGLILFERIIDFSSGSSLIIGRLPLVLSGM
                     YIFGLLICMDRLKEQPVKHLFYVGLFSFIFLTLPFLRFSSTIAFIDVGQGDSIFLQSP
                     FRKETILIDTGGQLHFEREKWAQGMIRPPAERNIVPYLKGQGISVIDKLILTHDDTDH
                     VGELPTLSQHFTIKKIYIGWGAGRNEPLQRHLSEAQKNGTEIIEIKHGDRISGYYDLY
                     VLTPFEKGEGRNEDSIGLWMEYNNRRFLFLGDLNQAMEKQLLLIYPNLKADVVKLGHH
                     GSRTSSNTDFLSHIEAKHGIISCGVNNRYGHPHSEVLETLEENQIKTWRTDQQGMITY
                     RWHPVFHPHGLVETMID"
     gene            24358..25404
                     /gene="holA"
                     /locus_tag="IV334_000985"
     CDS             24358..25404
                     /gene="holA"
                     /locus_tag="IV334_000985"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HAP8943075.1"
                     /translation="MVSNLSIQAELQKIRKEKLASCYLILGTEKFLQDQVRTEIVKKI
                     QLEGEDDLNFLSFDMENASLDEVVAEAETLPFFGDQRLVFVENPYFLTGEKVNNGIEQ
                     NTDLLTAYLKEPLESTVLVFFAPYEKLDERKKVTKQLKKTAIIINVQQLNEKEVRQYL
                     MNTLENSSIKMERSAIDLFLRLTDLDLSKMMRELQKLVLYGQNQQQITVREVEELVPK
                     TLEHNIFDMTQYILKGKTEQALRLYEDLVMQGEETIKINAILLSQLRLFLQTKFLVKI
                     GYQQANIAETLKIHPYRVKLAMQEVKKFDEHLLVQLFDQLVEMDYQIKTGQIEKELSF
                     QLFVLRTGQLLRNR"
     gene            complement(25493..25747)
                     /locus_tag="IV334_000986"
     CDS             complement(25493..25747)
                     /locus_tag="IV334_000986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HAP8943076.1"
                     /translation="MPNIESAIKRVRTSENANVKNSSQTSAMRTAIKKFEDAVASGAD
                     NVDALYKEAVKAIDMAESKGLIHKNKANRDKSRLSKKIAK"
     gene            complement(26147..26416)
                     /locus_tag="IV334_000987"
     CDS             complement(26147..26416)
                     /locus_tag="IV334_000987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8943077.1"
                     /translation="METFEGLLIDTYVLQEQPMMLTGINLQLEDRVINGLLKDRYLAK
                     YILRLPLNKYVLEVSGKWNRRNQLVIKKMLIKNIDEYIKIIGTPE"
     gene            complement(26886..27341)
                     /locus_tag="IV334_000988"
     CDS             complement(26886..27341)
                     /locus_tag="IV334_000988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF441 domain-containing protein"
                     /protein_id="HAP8943078.1"
                     /translation="MESWLFLGIILLVAILAKNQSLMIAAAFVLIMKLIPASRSFFPW
                     LQGKGINIGVTIITAAILIPIATGEIGLRDLLEAFKSPIGWVAVFCGGLVAVLSSKGV
                     GLIASDPQITVALVFGTILGVVFLRGIAAGPVIASGITYCILQVFALGK"
     gene            complement(27479..29407)
                     /locus_tag="IV334_000989"
     CDS             complement(27479..29407)
                     /locus_tag="IV334_000989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HAP8943079.1"
                     /translation="MTNQKKAFAATIGCLLFMIFGFIFDRFSLTITPLFYISAIIIGG
                     YKQTSEGLKELWNDRTLNVDLLMALAAIGACLIGNYFEGAMLTFIFCLSGALEEYTTN
                     KSQKEITALMNLQPQKAQLLKENGQMQEVDVSQLKVDNLVFVAKGAAVPIDGFLESTQ
                     ATIDESALSGESVPVEKYLGDEMYAGTLNQGNPITIRVTKTSSETVFAKIIQLVEEAQ
                     NTPTQTASFIERIENNYVKLILLAVPLMILLPHFFLGWSWDESFYRGMVLLVVASPCA
                     LVASATPASLAALSNSAKNGILIKGGIHLEKLSELKAIAFDKTGTLTKGKPVVTDSFF
                     FDEKDLAQQLLVAMEQKTTHPLAHAILQYFDCTIPEEIQYLPIEEITGFGLQTTYLGA
                     QWKVGKHAYDPETMIISKEIAEMIERLENQGKTVIYLSKDQQLIAVLGLLDIPKANTQ
                     QVISYFKSQNIHTSMITGDHSGTAKAIAEQVGIEHYYASCTPEEKTQLVKKENELYQV
                     NAMVGDGVNDAPALAAASLGVAMGQGTDAAMDIADIVLMKSDLNKFVYSHRLSLKMRR
                     IIKQNIIFSILVILLLIASNFLQFLNLPLGVVGHEGSTILVILNGLRLLRPLPDLSDS
                     SKKCKSCPLYKARLSSVK"
BASE COUNT         9868 a         5162 c         6257 g         8296 t
ORIGIN      
        1 taaagaaact aagaaaaaac cattatttgt acgtccgttc acgatgtgta aataaaataa
       61 atgtttgcaa caaaactgta taacaccaac gtttttaaat agaaacagat ggttgtaagt
      121 ttgattagaa aatagaaaaa atgcagatga aaaataaaac gctttcctta ttgtgatttt
      181 attcactaat gctgttaata tgcggattgg cataatttcc tttcatcgtg aaaatttgtg
      241 cttttagctt gtatttctca taaatccatg ataatttatt aatgaagacg gttaaatagt
      301 gatacagttt tattcactgt ttcaatcgca gaatgaagag attttcttat agatgagaga
      361 ggaatgtgtg acaaatggca aacaaaaaag caccgatcga cttccaagca ctgctagatg
      421 aagtcaatgc agatttccca gtatatcaag cacttgatca agatggaaac gtagttaaca
      481 aagatttagt acctgattta tccgatgatg aactcgttga attaatgact catatggtat
      541 ggtctcgtgt attagaccaa cgttcaacag cgctgaaccg ccaaggacgc ctaggctttt
      601 ttgcaccaac tgctggacaa gaggcaagtc agctggctag tgcttacgca tttgataaag
      661 aagatgttct tcttccaggt tatcgggatg tacctcaact agtaaaacat ggtttacctt
      721 tatctcaagc ttttttatgg tctcgaggac atgctgcagg taattcttat ccggaagaat
      781 tgaaagctct accaccacaa attatcatcg gtgcacaata tgtacaagca gccggtgttg
      841 cattaggttt gaagaaacga aacaagaaaa atgtcgtctt cacttatact ggtgatggcg
      901 gttcatcaca aggtgatttt tacgaagcaa tcaatttcgc tggcgcttac catgcaaatg
      961 ccgtatttta tatccaaaac aacgggtatg cgatctctac acctcgtgaa aaacaaactg
     1021 cagcaaaaac acttgcacaa aaagctgtcg cagctgggat tcctggtatt caagtcgatg
     1081 gaatggatcc gcttgcagta tatactgtat ctaaaatggc acgggaatgg gcgatcagtg
     1141 gaaatggacc agtcctaatc gaaacactga cttatcgtta tggaccacat acactatctg
     1201 gtgacgatcc aacccgctac cgtacaaaag acacagatga cgattggcag aaaaaagatc
     1261 cattgatccg cttccgtaaa tatttgacgg aaaaaggact ttggtctgaa gaaaaagaag
     1321 aacaagtcat cgaagcaact aaagaagaaa tcaaagcagc tattgctgaa gccgacaaag
     1381 taccaaaaca aaaggtttca gatttcttga aaaacatgtt cgaagtatct ccacaatcaa
     1441 tcaaagaaca aattgcaatt tatgaagcga aggagtcgaa ataatcatgg cacaaaaaac
     1501 aatgatccaa gcaatcacag atgccttagc acttgaacta gaaaatgacg agaatgtcgt
     1561 tgtttttggt gaagatgttg ggaaaaacgg aggagttttc cgggcaactg aaggattgca
     1621 ggaaaagttc ggagaagacc gagtattcga tactccttta gctgaatctg gaatcgcagg
     1681 attgagtttc ggtcttgcgt tagaaggatt tcgtccagtt cctgaaatcc aattctttgg
     1741 ttttattttc gaagcaatgg acgaagtagt agctcaaatg gctcgtacaa gatatcgaat
     1801 gagcggcact agaaatctac ccatcacaat tcgttcgcca tttggtggcg gtgttcatac
     1861 accagaattg cattcggata atttagaagg tttgatcgct cagtctcctg gtattcgagt
     1921 agtgattcct tccaaccctt atgatgcaaa aggactattg atttcttcta ttagaagtaa
     1981 cgatccggtt gttttcctag aacacatgaa actatatcgc tcattccgag aagaagtgcc
     2041 ggatgaagct tacgaagttc ctttggataa agcagcagtt actcgtgagg gaacagatgt
     2101 atctattatc acatacggag caatggtgcg tgaagcaatc aaagcagcag acaatcttgc
     2161 aaaagaaaat atatctgttg aaatcattga tcttcgtaca gttgcgccat tagatgtaga
     2221 aacgatcatt caatccgtgg aaaaaactgg tcgcgtagtt gttgtccaag aagcacaacg
     2281 acaagcgggc gttgccgcgc aagtagtttc tgagatctca gaacgcgcta tcctttcatt
     2341 agaagcccca atcggacgag tttctgcacc tgatacagtc ttcccatttg gacaagcaga
     2401 aaatgtctgg ttgccaaatg caaaagatat cgaagacaaa gtaaaagaaa tcgcggaatt
     2461 ttaagaaaag aagggacgaa acaaagtcat ggcttatcaa tttaaattgc cggatatcgg
     2521 tgaaggaatc gcagaaggtg aaatcgtcaa atggttcgtc aaaccaggag atacgatcaa
     2581 tgaagatgat acattactag aagttcaaaa tgacaaatca gtagaagaaa tcccgtcacc
     2641 tgttacagga acggttaaaa atgtgattgt tccagaaggt accgtagcaa atgttgggga
     2701 tgtattagta gaaatcgatg caccaggaca cgaagacaat gaaggggaca gtggtgtagc
     2761 cgcagaatcc caaactccag ctaaaccggc tgctgaaccc actgttgata cagaatcagc
     2821 tggatcgtca agcgaaggag tcttccaatt taagttaccg gatatcggtg aaggaatcgc
     2881 agaaggtgaa atcgtcaaat ggttcgtcaa accaggagat acgatcaatg aagatgatac
     2941 attactagaa gttcaaaatg acaaatcagt agaagaaatt ccgtcacctg tcacagggac
     3001 agttaaaaac gtgattgttc cagaaggcac tgtagcaaat gttggggatg tattagtaga
     3061 aatcgatgct cctggacaca atagtgcacc gtcaactagt gcgccatcag ctgaagcacc
     3121 aaaagaaaaa gtggaaacat caggttcagc aagtgtcgtt gaagctgccg atccaaataa
     3181 acgtgtattg gctatgccat ctgttcgtca atttgcccgt gaaaaagatg tagatatctc
     3241 acaagtaact gcaactggaa aaggtggcag agtaacaaaa gaggacatcg aaaacttctt
     3301 ggcaggtggt ccaagcagtg caccagcgaa atcagaagcg ccagaagcgg cagcaccgaa
     3361 agaagcagct cctgcagcag aaagcaaacc agctgcacca gcaaaaccat tcaagtccaa
     3421 cttaggagac ttggaagaac gtgtagcaat gacgccaaca cgtaaagcta tcgctaaagc
     3481 aatggtcaac agtaagcata cagctccaca cgtgacattg catgatgaag tagaagtgtc
     3541 taaactatgg gataatcgga aacgatttaa agaagttgct gctgcaaatg gtacgaaact
     3601 tactttcttg ccatatgtcg tgaaagcttt aacagcgact gttaagaaat acccagtatt
     3661 gaatgcatct atcgacgatg caaaccaaga aatcgtttat aaacattact acaatatcgg
     3721 tattgcaact gatacggatc acggactata tgtaccaaat gtgaaagatg cagaccgcaa
     3781 aggaatgttt gctattgcag acgaaatcaa tgagaaagca aaattagctc atgacggcaa
     3841 actatctgca gaagatatgc gcaatggaac aattacgatc agcaatatcg gatctgtagg
     3901 tggcggttgg tttacaccag tcatcaacta tccagaagta gctattttag gtgtaggaac
     3961 gattgcacaa caaccaatcg taaatgctga aggcgaaatc gttgtcggtc gtgtgatgaa
     4021 actgtcatta agtttcgatc accgtatcgt agatggagca actgcgcaac aagcaatgaa
     4081 taatatcaaa cgtcttttag ctgatccaga gctattaatg atggaaggat gataaacaaa
     4141 tggtagttgg agattttgct gtagaattag atacagttgt aattggtgcg ggacctggtg
     4201 gctatgtagc tgccatccgc gctgctgaaa tgggacaaaa agtagcaatc attgaaagag
     4261 agtatattgg cggtgtttgt ttgaatgtcg gctgtattcc ttcaaaagca ctgatagctg
     4321 caggacacca ttatcaagag tcactagatt ctgaaatgtt tggagtaact agtgaaaacg
     4381 tcaaactaga ttttgcaaaa acacaagaat ggaaagaaaa caaagttgtc catacgttga
     4441 cttctggtgt tggtttccta ttaaaaaaac ataaggttga aacaatcgaa ggagaagcgt
     4501 tcttcgtaga tgatcacaca ctacgcgtca ttcatccaga ttcagctcaa acttattcgt
     4561 tcaataacgc aatcatcgca acaggttccc gtccaatcga gattccagga tttaaattcg
     4621 gaggacgtgt gttagattct actggcggat tagcattgaa agaagtacct aagaaatttg
     4681 tgatcatcgg tggcggtgtg attggtgccg aattgggcgg agcgtacgca aacttaggag
     4741 cagaagtaac gattcttgaa ggctcaccac aaattttacc aacttacgaa aaagatctag
     4801 taaaattagt agaagatgat ttcaagaaaa aaggcgtaac agtcgtaacg aatgcaatgg
     4861 cgaaagaagc agtcgataac ggtgacagcg tgacagtgaa atatgcagtg gacgggaaag
     4921 aagaatccgt tactgctgat tatgtgatgg tcactgtagg tcgtcgtccg aatacagatg
     4981 atatgggctt agaacaagcg ggtgtcgaag taggagaacg aggattgatc acggtggata
     5041 aacaaggacg tacgaacgta cctaacatct atgccatcgg tgatatcgtt ccaggggcgg
     5101 cattagcaca caaagccagt tatgaagcaa aaatcgctgc tgaagcaatc tctggcaaaa
     5161 aagtagctgt agattataaa gcaatgccgg cagtagcctt cactgatcct gaattggctt
     5221 ctgtaggtat gacgatcaaa gaagcaaaag atgctggtat cgaagcaaaa gcatataaat
     5281 tcccattttc agggaacggt cgggcattat cgcttggtaa aacagaagga ttcatccgtc
     5341 tagttacgac gatcgaagat aacgtgctta ttggcgcgca aatcggtgga gtcggcgcaa
     5401 gtgatatggt ctctgaatta gctttagcaa tcgaatcagg aatgaatgct gaagatatcg
     5461 ccttgacgat tcatcctcat ccatctcttg gcgaaatcgt catggatgct tcggaattag
     5521 cattaggttt accaatccat atttgatttg aataatacag gcgaaacgca actgaaaaat
     5581 cagttgcgtt ttgtttttta gaattatcaa taatattatt atgtaaactt aaaaatatga
     5641 caacttcttg gtttattacc acttcctatg ctacactgaa gaggaatatg aaataggaga
     5701 gttgaagatg caagaatatc agtatccact tgatttagat tggacaacag acgaaatggt
     5761 cattgtaatg aagatgtggg aagatctgga acgtgcgaat gaaaaaggga taaacaatga
     5821 agcttttttg aagacttatc agcagtttaa aactgttgtc aaatccattg gtgaggaaaa
     5881 acgattggga cgggaatttg aaaaagcttc cggttattct ttatatcgta cagtgcagca
     5941 agcaaaaaaa aatccaaaca aaactttgag aatgaacggg tgagataaat ggaacagatg
     6001 attgatcata tcaagtcctg gcttgaagaa gctggtgaga aaatcaaatc ttctcaacaa
     6061 acattaacaa tctcgcaaaa atcaaatcga acggatctag taacaaatgt cgatcgaaac
     6121 atccagaatt ttctaatcga aaaaatccat tcctttgatc cagaggcgaa aatattggca
     6181 gaagaagaag ggtgtaatca attatcggat ctcgatggaa gagtctttat catcgatcct
     6241 attgatggca ccttgaattt tgtgattgaa ggggaaaatt tttgtattat gctcgctgtt
     6301 tatgaatccg gcgttggaaa gcttggtttc atttatgacg tgatgagaaa tgaacttttt
     6361 tggggtggaa aaggaatagg cgtgtataaa aatgatgagc cattgcctag accagcaaat
     6421 aaatccttat cggaaggctt acttggtgtg aatagttatc ttttcggtca taataaatat
     6481 ggaatccgag aaattgcgga aagcagcatg gggacacgta tgtacggatg cgctggtctt
     6541 gaactagtgg caatcttgaa aggaaaccac attgcttata tttcaaacct tagcccttgg
     6601 gattatgcag cgggaaatgt cttattggaa gagtttggta tggtaacaag cggtcttttt
     6661 ggtactcctt tgacatttga aaaaagggaa tactatttag cagggacttc ttgtgcatat
     6721 gcagaaatcg cagacctttt gaaaaaagaa ggctgaaaaa aaggtagaaa tcattcacaa
     6781 aaatgataaa aacgagcaaa agattaattt tctcgaaaaa atgaaaacat tttactatat
     6841 ttcatgaaaa aagtctgcta taattagcag tcgagtaaaa tttgccctgt cataaagaac
     6901 caataaaaaa ggagttagtt acattgaatt atagaaacga tatccgtaac gtcgcaatta
     6961 ttgcccacgt tgaccacgga aaaacaacat tagttgatga actattaaaa caatcagata
     7021 cattagacgc acatacgcaa ttgcaagaac gtgcgatgga ctcaaatgcg ctagaaaaag
     7081 aacgtggtat cacgatttta gccaaaaaca cagctgtaga ctataaaggt attcgcgtaa
     7141 acatcatgga tactccagga cacgcggact tcggtggcga agtagaacgg atcatgaaaa
     7201 tggttgacgg cgttgtgttg gtcgtagatg cgtatgaagg aacaatgcct caaacacgtt
     7261 ttgtattgaa aaaagcactg gaacaacata tcacaccaat cgttgtcgta aacaagattg
     7321 acaaaccttc tgctcgtcct gagcatgtcg tagatgaagt tcttgaacta ttcatcgaat
     7381 taggagcaga tgatgatcag ttagactttc ctgtgattta tgcatctgca ctaaatggta
     7441 cgtcaagctt gtcagatgat ccagcagatc aagaaccaac catggcacct atttttgata
     7501 caattattga aaaaatccct gctcctgtcg ataacagcga cgaaccatta caattccaag
     7561 tttcattact tgactacaac gattatgtag gtcgtatcgg aatcggacgt gtatttagag
     7621 gaacaatcaa agtaggagac caagtagcat taattaaatt agacggaaca gttaagaaat
     7681 tccgtgtaac gaaattattt ggtttctttg gactaaaacg tctagagatt caagaagcaa
     7741 aagctggaga cttgattgct gtctcaggta tggaagatat ctttgtcggt gaaacagtta
     7801 ctcctgtgga tcatcaagat gcgttaccta tccttcacat tgacgaacca acattgcaaa
     7861 tgactttctt agttaataat tcaccatttg ccggtcgaga aggtaaattc gtaaccgctc
     7921 gtaagattga agaacgtttg atggcagaac ttcaaacaga tgtatcctta cgagttgaac
     7981 caacaaactc tccagatgca tggacagttt ctggtcgtgg agaattgcat ttgtctatct
     8041 tgattgaaaa catgcgccgt gagggatacg aattgcaagt atctcgtcca gaagttattg
     8101 aaaaagaaat cgacggtgta aaatgcgaac cgtttgaacg cgtgcaaatc gatacaccag
     8161 aagaatatat gggtagtgtt atcgagtcct tgagcttacg taaaggtgaa atgcaagata
     8221 tggtccatac aggaaacgga caaatccgtt tgacattctt aacacctgcc cgcggtttga
     8281 ttggttattc aactgaattc ctttcaatga cacgtggtta cggaatcatg aaccatactt
     8341 ttgaccaata tctgccaatg cttcctggtc aaattggcgg acgtcaccaa ggggcgcttg
     8401 tttcgatcga tacaggaaaa gcaacgactt attctatcat gagtatcgaa gaacgcggta
     8461 cagtcttcgt agaaccaggt acagaagtat atgaaggaat gattatcggg gaaaacagcc
     8521 gtgataatga tttgactgtc aacatcacga aagcaaaaca aatgacaaac gtacggtctg
     8581 ctactaaaga ccaaacatct gtcatcaaaa aaccaaaaca attgacgttg gaagaatctt
     8641 tggaattctt gaatgatgat gaatattgcg aagtaacacc agaaagcatt cgcttgagaa
     8701 aacaaatttt agagaaaaat gctcgtgaaa aagcatctaa aaagaaaaaa taatctgtaa
     8761 aaggatgaag agagaaacca gcttgttata aaaaatagct ggtttctttt tttgtccaac
     8821 tcttttctgt ggagaagtca tctttgtcaa tggacataaa ttcagaaaat acgccgaaag
     8881 acgtttaaaa gacttttaaa aaacttattt ctagagagtt tttgtttcat aataagataa
     8941 tgtttcacga aaggagatca acttgtcctt cgaagaaaat cgaagtaaaa tggcaagaga
     9001 aaggagcggg aagatgaacg acttattaaa acaatacttt ggttatgatg agtttagacc
     9061 tggtcaaaaa gaaattatcc agaaagtcat tgatcaggaa aatgtattag gaatcatgcc
     9121 taccggaagt gggaaatcaa tctgttacca gcttccagct ttactattag atgggcttac
     9181 tgtggttgtg tcccctttaa tctctttgat gaaggatcag gtggacgcgg cgaatcaatt
     9241 aggtataccc gccactttta tcaatagctc gttagatggt tacgaaacag ctagacgctt
     9301 ccaagaaatc gatagacagc agtaccgttt actttatatc gctcctgaac gttttatcat
     9361 gccggatttt atccaagcaa tgaagcgatg gaacgtccgt atgatcgcta ttgacgaggc
     9421 acattgtatc tctcagtggg ggcatgattt ccgcccaagt tatttacaga tggcgaacca
     9481 gctagatcaa ctgccaaatc gtcctgtaat tgttgctttg acagctacag cgactgtgca
     9541 agtagctgct gacataaaaa gacttttaaa aattccagaa aacaaccata ttcagactgg
     9601 ttttgaacga gaaaatctac gatttcaagt aattaaagat caaaaaaaag aacaatatct
     9661 aatcgaatat ttaaagatca acaaaaatca atctggtatc atttatgcag caacgagaaa
     9721 agaagtagac cgtctctatc atttgcttaa aaaattcgat ttttccgtgg gtagatatca
     9781 tggtggtcta aatgagaatg agcgaacaga aatgcaagaa gcctttttat acgatcgctt
     9841 gcagctgatt gtagcgacaa atgcatttgg tatgggtata aataaaagca atgtccgttt
     9901 tgttatccat tatcaaattc ctggctctct agaagcatac tatcaggaag ctggtcgggc
     9961 gggaagagat ggtctttcca gtgaagccat cctgcttttc tctcctcaag atatccaagt
    10021 ccaaaagttt tttatccagc agtcccaaag agaagaaggg caaaagcaaa aagaatatga
    10081 aaaattaaaa gcgatgacag aatacgtgta tattgagtcc tgtcttcagc aatacatcct
    10141 aaactatttt ggcgagacaa gcagtccgtg caaccgttgt ggtaactgtt tagatgacag
    10201 agaaatagta gaagtaacga cagaagcaca gatggttctt tcttgcttaa aacgtatggg
    10261 tgagaactat ggtaaacaaa tgctgatgaa agtcttagca ggatcgaaag aacaaaagct
    10321 gcaagcttta ggttttggac atttatctac ctatggactg atgaaaaacc aatcgcaaaa
    10381 agaaacgatg cagttaattg aatatttgat atcgaatggc tatcttctga ccatcaatgg
    10441 tgagtatcct gttttgaaag taacagaaag agggatacaa gtattgaaag gacaagaatc
    10501 tgtttatcga aaggaaccca agaaagtcca gcagctatcg gatgaagaaa ccgatacact
    10561 atttgaagtg ctgcgggaac tgcgaacgga tctagcatca gaagcaggcg ttcccccgta
    10621 tgtcgtattt tcagattcca cattgaaaga aatgagccga atacgtccat caagcagatt
    10681 ggagatgctt cagataaaag gagttggcca aagcaagctt gataaatatg gcgaagcctt
    10741 tttatctcgg ataaaaaatg cggactctaa cgttctggat aagtagaaag aacgaatacg
    10801 taaaaatgaa taaaaacaac tggttaaatc tgtttttttt gctccttatg aaaatcaaat
    10861 atgttataat taaggaatta gaatgggagg taagcacaga tggattcttt gtcgaaaaaa
    10921 cttgcgatcc aagtattaaa tgatgaagca gaacggatca aacgattaat ccgaaatcaa
    10981 aacaacagtc tctgtatctc acaatgcaaa gccttcgaag aagtggtaga tacacagatg
    11041 tacggctttt cccggcaagt cggttttgct gtccgtatag gagtaatcgg aaagaacgag
    11101 ggccagctga tgttaagtga gctagaacga gaattgaatc agttatatag tgaggtttac
    11161 caagaaattt atgataaaaa agaagttggc aaggaggaat agacttgcct aagaaagtga
    11221 aaaaaaggca tctattagac tatagcatct tgattcccta tttaatttta tgtgtattgg
    11281 gattgatcat ggtatatagt tctacgtcgt atctattatt agaaaacgga caaaatccgt
    11341 ccgcatcagt cattaaccag agtatttttt gggtgcttag cttgattgct atcgcactac
    11401 tttataaaat gaaaacagat gttttgaaaa accagcgttt gatcatggca gccatcgcag
    11461 ttttgacgat tttgctgctg attgttgtgt tttttggtaa ggagatcaat ggggcgaaag
    11521 gttggctgca aattgcagga ttctcgatcc aacctgccga atacctaaaa atcatttcta
    11581 tctggtacct gtctctgact ttatctaaaa gacaaaacag tgtacaaaaa gattttcttg
    11641 ggactgtgaa acgtccactt gcaatggtta tcggacttac tgcattagtc gctattcttc
    11701 ccgattttgg taatgcggca gttatctttt tgattatttt ggttctgctt ttagccagcg
    11761 gagtcaatta tgtgtatact ttgattgtag gagtaggagg cttttgtcta agtactttta
    11821 cgatctggct gatcaatatc acaaacggaa agatcttacc cggacgttta cagtatatct
    11881 ataatcgatt tgctatttac caaaatcctt tctcagacga attaaataaa gggcatcaac
    11941 tagtcaacgg ctactatgcc atgttcaacg gaggactatt tggcagggga ttaggtaaca
    12001 gtatccagaa aaaaggattt ttacaagaag cacaaaccga ttttatttat gcaatcgtag
    12061 tggaagaact gggagtgatt atggggattt tgatattagc gttgctattc tttatgattg
    12121 tgcgcattgt ccttgtaggt atccgttcaa aagatccgtt caattcgctt ttgtgcattg
    12181 ggatcggcgc aatgtttttg atccaagtat ttgtcaatct tggaggaatt actggcgtta
    12241 ttcctttgac agggatcacg ttcccatttt taagtcaagg aggctccagt ttgctgatgc
    12301 tttcgatttg tgtcggcttt gtcctcaaca tcagtgcgga tgaaaaacgc aagagcttag
    12361 gtttataata aaaaaagatg taggagtggt tcgatgaaaa aagttttagt tgctaacagg
    12421 ggtgaaatcg ctgtacgtgt atttcgtgct tgtacagaat taggaattaa aactgttgga
    12481 atctatgcag aagaagatga atattccgta caccgtttca aagcggatga agcatattta
    12541 gtaggacaag gaaaaaaacc gattgatgcg tatctagata ttgaagggat catttctatc
    12601 gcgaaagaat gcggagctga cgctattcat ccgggttacg gtcttttgtc ggaaaacttg
    12661 aattttgcgc aacggtgcga agaagaaggc atcatttttg ttggacccaa actgcatcat
    12721 ttagatattt ttggagataa aatcaaagca aaagctgcag ctatcgaagc ggggattgct
    12781 tcgattccag gcacagatgg accaatcgct tctatagacg atgccttgga gtttgcgaaa
    12841 caatacggtt atccgattat gatcaaggca gctctaggcg gcggcggacg cggcatgcgt
    12901 gtagctcacg atgaaaagag tgcaagagag ggctacgaac gtgcgaaaag cgaagcaaaa
    12961 gcagcctttg gtagtgatga agtgtatgtt gaaaaataca tcgctaatcc taagcatatc
    13021 gaagtacaga ttctcgggga tacgcatgga aacgtcatcc atctttttga aagagattgt
    13081 tctgttcagc gccgtcacca aaaagtagta gaagtagcgc cttgtgtctc tatgaatgaa
    13141 cagcaacgtc aaaaaatctg ccaggcagct gtccaattga tgaaatatgt cgggtatgtc
    13201 aacgcaggta cagtagaatt tcttgttgaa ggagatgatt tttactttat cgaagtcaac
    13261 cctcgcgtcc aagtggaaca tacgatcaca gaaatgatta cagatattga tatcgtaaca
    13321 acgcaactgt tgattgctca aggtttggat ctgcataaag aaatcggatt gcctcagcaa
    13381 gaaggaatta aattaaatgg ttcagccatt caatgccgaa tcactacaga agacccgttg
    13441 aataactttt taccagacac agggaaaatc gatacgtatc gttcacctgg tggttttggc
    13501 gtacgtctgg atgtcggaaa tgcttacgcg ggctatgtag tgacaccgta ttttgattca
    13561 ttgctagtaa aagtatgtac gcatggtgct acctttgaaa cagccattca gaaaatggaa
    13621 cgttgcttga aagaatttag aatccgtgga gtgaaaacaa acattccatt tatgctcaat
    13681 gtcatcacac atcctgaatt ccaatcagga aatgcaaaaa caaccttcat tgatagtaca
    13741 gcaacattat ttgaatttcc tcgactacga gaccgcggaa acaaaacgat gaaatatatc
    13801 ggtgaaatta ctgtgaatgg ttttccaggt atcgaaagtg gcgaaaaacc attttatgaa
    13861 gaaccacgta tgcctaaaga tctaatcaca agaactgatt acgtaacggc taagaatgta
    13921 ttagatgcga agggtgccga tgctttggtc gaatggatca aaggacagga aaatttgttg
    13981 ttgacggata cgacatttag agacgcccat caaagtctgc tagcaactcg tgttcggaca
    14041 aaggatttca agcagattgc acgtttgact ggagaaggtc ttcctgaact cttttcaagt
    14101 gagatgtggg ggggagcaac atttgatgtg gcttaccgct ttttgaatga agatccttgg
    14161 caacggttga gaaaaattcg ttctttaatg cctaatacgt tgctgcaaat gcttttcaga
    14221 ggttcaaatg ccgttgggta ttctaattat cctgacaatg tattggtgga atttgtcaaa
    14281 gaagcagcag cgcaaggaat cgatgtattt cgaatctttg atagtttgaa ctggactcca
    14341 caaatggaaa aaagtatcca agctgtacgg gatactggta aaatcgcaga ggcagctatt
    14401 tgctatactg gcgatatcaa cgatccaagt cgggcaaaat acaatgttca gtattataaa
    14461 gacatggcaa aggaactaga acaactgggt gcgcatatca ttgcaatcaa agatatggct
    14521 gggctgctta aaccgcaggc agcttatcgt ttgattagtg aattgaaaga aacgacagat
    14581 ctgccaatcc atctgcatac tcatgatacc agcgggaatg gaatcatcac ttattctgct
    14641 gcttcaaaag caggagtaga catcgttgac gtggcaatga gtgcaatgag tgggaatact
    14701 agtcagccta gcatgagcag cttgtattat gcactggtca atggtccacg gctgccagaa
    14761 atcacgattg aaaatgcaca gaaattaaac cattattggg aagatgtgcg catgtattac
    14821 aaaccgtttg aaaacggctt gaatgctcct gaaacagaag tatatatgca tgaaatgcca
    14881 ggaggacagt attcgaatct acagcaacag gcaaaagctg tcggattagg tcatcgctgg
    14941 gatgaaatca aacaaatgta tcatactgtc aacttgatgt ttggcgatat cgttaaagta
    15001 actccatcat ctaaagtagt tggagatatg gcactattta tggtccaaaa tgatcttaca
    15061 gaagaagata tttatgaaaa aggcgaaaca ctaagtttcc ctgaatctgt tgtgactttc
    15121 ttccaaggag aactagggca acccgttggc ggatttccta aaaaattgca aaagattatt
    15181 cttaaaggca gacctgcatt gaatgaacgt ccaggtcttt tggctgaatc agtcgacttc
    15241 aacgaagtaa aaaaagaatt ggcagaaaaa attggctatg aaccaaaaca agacgaagta
    15301 ttaagctatt tgatgtatcc gcaagtcttt ttggactatc aaaaagcata taatcaattt
    15361 ggcgatgtta cattactgga cacaccaaca tttttccaag gtatacgttt gggtgaaaca
    15421 atcaatgtac aaatcgaacg cggaaaaacg cttatcattc gtttagatga aatcggagaa
    15481 cccgatatcg aagggaatcg cgtactgttc ttcaatttga atggacaaag acgtgaaatc
    15541 actatcaaag ataattcgat catcagtacg gtccaaaccc gattgaaagc tgaaccgaca
    15601 aaccgagaac aaattggagc aacgatgtct ggttctgttt tggatgtatt agtgaaaaaa
    15661 ggcgataacg ttaaaaaagg tgatacgttg atgattactg aagcgatgaa aatggaaacg
    15721 gccatcgaag cacgttttga cggagaagtc gcacatgtat atgtgtcttc aggagacact
    15781 atttcgtctg gtgacctttt gattgaagtg actgagaaat aataagcgaa aggtggggaa
    15841 acatgaaaag actgggtttg ttttggctga ttctgctatt agttattgtg gcattctaca
    15901 tggaaccagt cttgtttccg ccaaaacttg aagtgaagga aaactctgat caacaaatat
    15961 caaaccaaat gacaacgtgg aaataccagg agcttaaagc aactggtttt tctacttata
    16021 tcggaaaacc gatcgatcag ctggaaaagg attttggcca agcctatgat cgattaacta
    16081 gcggatttgg ttttgaaaca agatattatg aaaataaagc tagtcatctc acatttgaag
    16141 cgaacatcca agacagcaga gtcacaactg ttaaagtact gcaaacaggg cctgctgata
    16201 tcgcgccgtt ttatttaggg atgacgatgc aagatcttac gaaaatcact actatctata
    16261 ctaatttcac ttttgagtac catgatacag aagttggaat cgaactaatg gaagaggata
    16321 tgaattatcg tccgctaatt gcttttgata acgacacttt tgctatttta ttttttgatc
    16381 aaacaacaaa cggcctattt tcagttgtgt atttagataa agatagtctt ttgaaactat
    16441 tgccatatca agtgtttggt gaaggccttc cacattatca agcagataaa tcggctgatt
    16501 gggaatcaat caatcgcgtt aaagaacgaa aaagtacgac tttattaaat atgcttagaa
    16561 gagaagacga acttcccata tacaatcaaa cacttgcctt tcaaaaccac agtcagctgc
    16621 tactacatga ttttttgaca aggcctgagg aaatattgtc agaggaacgt atggctgatt
    16681 ggcaaaaatc gctaggcagt gctcaaacga cagtgaaatt ctcactgacg aatgatgaat
    16741 tggagaagtt aatcagtaaa caaaaattag aaaatactag tgggctattt attcatccgg
    16801 cgattgacgc tactttttcg tttttatatt ggtacagcga tccttacaac catgaccgtt
    16861 tcatggaccc gggaacggat tctgtaggca ttgctttttc taaagaaaat atgttagtct
    16921 tattgcaaga agaaaacgga gaaagtagtg atagcagtga tcgttaacga agagctgttt
    16981 caactggagg atcaatgcct ccaagtcgct gaaatgataa aaaagagtaa aacgatggct
    17041 tcttatcttg aacataaaca gtcaatggaa gaatctgcaa aagtttgcca gttgaaagaa
    17101 gcgttcttgt ctaagaaaga agcatttgaa aatgtagcgg catatggtga gtatgcacct
    17161 gattatcgtg aaaaaaaacg agctgttcga aaagcaaaaa gacagcttga tatggatgag
    17221 catgttgcag ctttccgata tagtgaaaca cagctgcaaa gtctgttgga tcaaattgtc
    17281 caagtacttg ccgaagaagt ttctaatgaa atcaaagtag atgcaggtag cccgtttttt
    17341 gaaacgaaaa aacactttgg atgcggaggt aattgtcatg caattagaca atagtgaatt
    17401 tgaaataaca aaacgacgcg ggctgatcgt atgggtctac agcttaaaac aattaaagaa
    17461 tctgaaaaag ttcggcttgt tgcattatgt ctcaagaaga atgaaatatg tcgtgcttta
    17521 cttaaatgaa gagacattgg aagaaacaga agaacgaatc cgtaaattac attttgttcg
    17581 aaacgtagaa cgttcttacc gaccggatat cgaaatgaat tttgccgaaa aaataggaaa
    17641 caaaacagta ttaaaagacg aaggtttcga aattgaagag ctaaatacag aaatccgatt
    17701 agctgaacat ctttaaaaat ctcttatgag cagtttttaa ataaaaaata acttggataa
    17761 cgatcgattt ttgatcattg tccaagtatt tttttggtat actagtacca gtaccatttc
    17821 taaggagtga gtagatgcgg ataatatcag gaaattatgg cgggcgccgt ttaaaaagtt
    17881 tagcaggggc gaacaccaga ccaactacag ataaagtaaa agaatccata ttcaatatga
    17941 ttggaccata ttttgatgga gagacagtat tggatctgtt tgcaggaagc ggtggtttag
    18001 ctatcgaagc agtatctaga ggctgcagtc atgcggtatg cgtagacaaa aattaccaag
    18061 cgctaaaaat catcaaagaa aacatcgaaa ttacgaaaga accagagaaa tttaccacaa
    18121 taaaacttga tgcggacaaa gcaatcgaac ggctagcagc ggagaaacaa caattcgact
    18181 acttgtttct tgatccgcca tatgcaaaac aaaaaataat cgatcaaatc cataaaatgg
    18241 aagaactggg acttttttct aaaaccgcag ttatcgtatg cgaaacggat aaaacagtcg
    18301 aattgccaga aactgtggct ggattccatc aaataagaaa acagatttat ggcataacgg
    18361 ccgtaactat ctatagaaaa gagggaaatc aatgaaacgt gcattattcc caggaagttt
    18421 tgatcctttt acaaaaggtc acttagatac ggtggaacgt gcggctaaat tattcgatga
    18481 ggtagtaatt ggtgttttta tcaatacttc aaagaaaagt ttgtttcccc cagaagaaag
    18541 aatgacattg atcacaaaag ctgtttctca tcttcctaat gtaaaagtca tgcatcaaga
    18601 aaatcaattg acggtagaaa cagcaaaaga gattggtgca gatgcgttga tcagaggcat
    18661 acgaagtatc aaagactttg aatatgaacg agagattgct cagatgaatc atcatctata
    18721 tgatgaattg gaaactgtct tcttattagc aaaaccagaa tatagtcacg tgagttccag
    18781 tattttaaaa gaagtactgc actttggcgg agatgtgagc agctacttac cgccagtgat
    18841 caacgaagct ttagctggaa agcgtgaatc aaatgaaagc taaacaatgg ttgaaaacag
    18901 tactgattat catatttgga atgttgatca ttggatttgt agtagttccc ttgccgtatt
    18961 atgtagaatc accgggagcc acgatcgatc tgaaagaact aattacggtc aatgataagg
    19021 aagataaagg ttctgggtct ttctctctta cctctgttgg tatcaggcaa gctacaggtt
    19081 tcttagcttt aaaagctaaa atgacccctt ttgaagaatt gatcagtgaa gaagaattga
    19141 ctggtggggc gacaaatgaa gaatataatc aaatgcaaca gtattatatg gattcttccc
    19201 aaaatgcagc aatcgaacaa gcgttaaaac ttgcagacaa gccttataaa atggaattca
    19261 aaggcgtata tgtcatgagt gtagagaaaa catcgaattt ctttggcaag ctttcagttg
    19321 gagatacagt aacaaaagta gacgggaaaa gctttcaaag tacagaagca tttatggatt
    19381 atgtaaaaag ccaaaaagtt ggacaaacaa tcgaaatcag ttatttgcat aatggtgaag
    19441 aaaaaacagc ttctggtgat ctgatcgagt taccaacaga taaaaaggca ggaataggta
    19501 tcacgcttac tgaccatacc gagatcgaat cagatacaga tgttcgtatt aattctggct
    19561 ctatcggtgg gccatcagcg ggattgatgt tcactttaga aatctatgaa caagtcactg
    19621 gtaaaaactt gcgacatggt aagcaaattg caggaactgg aacgatcaac agtgaaggag
    19681 aagttggccg aatcggagga atcgataaaa aagtggcaag tgcggataaa gctggcgtag
    19741 agattttctt tgcaccagat gatacgattt cagacgatgt taagaaagaa tatcctgaaa
    19801 taaaaaccaa ctacgaagaa gcaaaagctg ccgctaaaaa attgaattcc tcaatgaaaa
    19861 tcgtaccagt aaaaacagta caagaagcgc ttgactatct agagaaaatg aaatgaaact
    19921 aaataattaa agaggggttg tgacacgagt tctgtcacaa cccctctttt tcgtcaaaaa
    19981 tcttcacgat aaaaataaaa acaaaagaaa aaattgacgt aattagatgt tgaagggacg
    20041 attttgccag agctcactgt ttcttcaatt ttaaatgaaa ggagtaatga atgattttta
    20101 ctggtcattc tttgaaaaaa gaacaatgat agaaaaaatc agcaagaaaa tactagcttc
    20161 acccaaattg atcatttgga tgtctatgat agttcttatc ttttgtatcg caagtggttt
    20221 tttattatca aatttgctat tttatagaga aacagaagag actgctgcaa aagaaatact
    20281 ttcttcgttt agttccagtg gagaagatcc atcagctgaa ggaaacgatg tagaagagga
    20341 tgctcgtcca aaggtcatgt acaccgatat caaaggaagt gtgaaggagc ctggtatcta
    20401 ttccttttct tcagaagaac gggtctatga tgtactaaag cgtgcaggtg gattattaga
    20461 agaagcggat agcgatcgaa tcaacttttc tgcgaagata gaagatcagc aagtcctata
    20521 tatccctgct gtaggtgaag agctgccaga acatcttgat cagttggcat cctcggaagg
    20581 aaaacagcct acagcagatt ccgagcctcc caaaataaat atcaatacag ccactccatc
    20641 agaacttcag cagattcctg ggatcggatc agtcaaagcg caagaaatca ttcgatttag
    20701 agaagaaaac ggatccttcc agaaagtgga agacctgcag gagatttctg gtatcggtga
    20761 aaagacagtt gaaaaactaa aaaacttcgt tacaattaaa tagaatgatt caaaatcgac
    20821 tggcacgagc aataaatgga cgctaatgga tactaagaat aatccaattc taagagcttg
    20881 gcaggacgag gaggtagtat ttttggatag gaaatagctt gattgttcgc aaagaaacat
    20941 taaaaaaacc aatgctgaat gtctatctcc aaaataaaat atctgggatt catattatga
    21001 atacggctgt cagcggcaat aacagtcaag ctctacgaga acgctttgcc aaagatgtac
    21061 ttagctatac agcagataaa gtatttattc tcattgggac gaatgatcta gcagagcata
    21121 aacaactttc taaagaaacg taccaaaaaa tctgcagtgg atgatagttc agctgaaaaa
    21181 agactatagt tctgatcatg ctttctttat cacgccacca gcagtcgatg agacaaaaca
    21241 aaaatatcgg aataacaagt tgatcaaaga atatatctct gttctcacag aagtagcaaa
    21301 acgccatgat tgtgaagtat cgatttttat acggaatttc ttgaacaaat ggattcgaag
    21361 aatttttata atgggctaat ggatgacgga cttcatttcg gcagtgaagg gtaggatctt
    21421 ttgtcagaca ggatgattcg ttatatttaa agaatatttt gtttaagcaa tgagtacttt
    21481 ttaatcaact tgaaaaaacg gaggaatata aaacatgtta gaaaaatctt caaaagcatg
    21541 cgatacgaat aaagaaaaag ttactgataa gtcacataat gaacgcattc cttgggacca
    21601 gtactttatg gctcaggcag tactgttatc tttacgtagt acgtgtacac gtttagaagt
    21661 aggagcgaca ctcgttcgag aaaaaaggat cattgctggc ggctataatg gagcagtcag
    21721 tggagatgtc cactgcatag atgaagggtg ttacgtcgtg gatggacact gcttgcgaac
    21781 gatccatgca gagatgaatg cactcctaca gtgtgcaaaa cttggtatcc cgacggaagg
    21841 ttcagaaatc tatgtcacac attttccatg tttggcctgt acaaaggctt tgttgcaagc
    21901 tggtgtgaaa gggatcaatt atctccatga ctatcgaaat gatccttatg cccaagcgtt
    21961 gatcgaacaa ttaggtgtat ctgtacacca agttgcttta gactcacagc atttttcaaa
    22021 acttcaaaat gaattaacag gaaaacatgt ctagcgtttt agaaaggcat gttcgaaatc
    22081 ggttgatttt tccggcaatt acgtcgatga ttatctgttt gttgtattac actgacaatt
    22141 ggggttatcg tttgcttctt gccggaattt ttttatggtt ttcgctaaaa gaaggacgtt
    22201 ttatcgcgta cttgtgtatt ttgtgtagca taacggtatc catttcttat aggctacaag
    22261 agtattcatt tttagaggaa aaaacaacat ctgcgcaaat tactgtattt acagatacga
    22321 ttcgtgcaaa tggtaatttt ttgacaatgg aaggaaggtt atcagagaat aaacaaaagc
    22381 tccagctttc ttatcaagta aacacagaag cagaattgaa aacatggctt aacttccgag
    22441 gaaagggaat agaattgact gctgaaggcg agttcatctc tccagcaaaa aagcggaatc
    22501 tttataattt tgaccaagaa gcttacttca ggaaccatag aatttctgga cggttccaaa
    22561 ttcaaaagat tgaacagatc accatgcaaa atcactggcg aaactggctt cagagaaaaa
    22621 gaggatcttt tatctccttt gtccaatctg aatttccgca aaaaacaagt gtatatatta
    22681 attctttatt atggggctat aaagatgcag gattcagaga accagaagaa atttttaggg
    22741 agagtggttt gctgcatctg ttcagtctat cgggtctaca catccaattt tatttagggt
    22801 ggctgtatta tttatttcga cgtatcggat ggccactaca cgtcagtatt attccgttga
    22861 gtctactaac tatctgttat gtttctattg caggaagttc tatttctgtt ttgcgtgcta
    22921 gtttgctgtt tttattgcga ttgctagtca aattgctgaa taagcattat tccaccatgg
    22981 acttatttgc agttgtcttt tggcttcttt tactatttga tcctcgattt ctttttcatg
    23041 cgggtggaca gcttagtttt ttcatgagtt ttttgtttat cctgatttct tttgatggag
    23101 attggttgaa aaaaacggcc tctcacgtcc tgtttactat gatcacgatg ccgttgattg
    23161 tatggcattt ttatgagtgg cctattttag gtagtctttt tacattactg attgcaccgg
    23221 tattcaagtt tctttttctt cctggtgtgt tatttctcac actcttcaat cgctggctgc
    23281 cggaaacttt acttttgctg atagaagagg gattaatctt atttgagcga ataatcgatt
    23341 tttcttcagg gtcgtctctc attataggac gtttgcccct tgtgctatct gggatgtata
    23401 ttttcggatt actgatttgt atggataggt tgaaagaaca acctgtgaaa cacttgtttt
    23461 atgttggtct tttttctttt atttttttga ctttgccatt tttacgcttt tcttcaacga
    23521 tcgcttttat cgatgtcggt caaggagaca gtatcttttt gcaaagtcct tttcgaaaag
    23581 aaacgatttt gatcgatact ggaggtcagt tacactttga aagagaaaag tgggcgcaag
    23641 gtatgatacg tcctcctgct gaaagaaata tcgttccata tctaaaaggt caggggataa
    23701 gtgtaatcga taaactcatc ttgacacacg acgatacgga tcatgtggga gaattaccca
    23761 cgttatctca gcattttact atcaaaaaaa tttatatcgg ttggggcgct ggacgaaatg
    23821 agccattaca aaggcattta tctgaagctc aaaaaaatgg tactgagatc attgaaatca
    23881 aacatgggga caggatcagc ggttattatg atctttatgt attgactcct tttgaaaagg
    23941 gagagggaag aaatgaagat tcgattggtc tatggatgga atataacaat aggcgctttt
    24001 tatttttagg agatttgaat caagcaatgg aaaaacaatt gcttttgatt tatcctaatc
    24061 tgaaagccga tgtagtcaaa ttaggtcatc acggtagccg tacgtcaagt aacacagatt
    24121 ttctttccca tattgaagca aaacatggga ttatatcctg cggcgtaaat aatcgttatg
    24181 gtcacccgca cagcgaagta ttggagacac tagaagaaaa tcagataaaa acatggcgaa
    24241 cggatcaaca aggaatgatc acctatcgct ggcatccagt ttttcatcct catgggctag
    24301 tggaaacaat gatagactag taattctcat ctgctcatgg taggataatt tttgaaagtg
    24361 gtgagtaatt tgagtataca agcagagtta caaaaaatac gtaaagaaaa acttgcatct
    24421 tgttacttga ttttaggaac agaaaagttt ctccaagatc aagttcggac agaaattgtc
    24481 aaaaaaatcc aattagaggg cgaagatgac ttaaattttt taagttttga catggaaaat
    24541 gcaagtttag atgaggtagt agctgaagca gagacgttgc cgtttttcgg agatcaacgg
    24601 ttagtattcg tagagaatcc ttatttttta acaggtgaaa aagtaaacaa tggaatcgaa
    24661 caaaatactg atttattgac ggcttatttg aaagaaccat tagaatcgac tgtattagta
    24721 ttctttgcgc catatgaaaa gttggatgaa cggaaaaaag taacgaaaca attgaaaaaa
    24781 acagcaatta tcatcaatgt ccaacagttg aatgaaaaag aagtacgtca atatctgatg
    24841 aataccttag agaattcctc aatcaaaatg gaacgaagtg caattgattt attcttgagg
    24901 ttgaccgact tagatttatc caaaatgatg agagagttac agaaattggt cctttatgga
    24961 caaaatcaac agcaaatcac ggtaagagaa gtagaagaat tagttcctaa aacgttagaa
    25021 cataatattt ttgacatgac ccaatatatt ttaaaaggga agacggaaca agctttgcgt
    25081 ttatacgaag atttagttat gcaaggggaa gaaacaatca aaattaatgc gattttgctt
    25141 tctcaattac gtttattttt acagacaaaa tttcttgtaa agattggtta tcagcaggca
    25201 aatattgctg aaacattgaa aatccatcca tatcgtgtaa aacttgccat gcaggaagta
    25261 aaaaaattcg atgaacattt gctggtacag ttgtttgatc aattagttga gatggattat
    25321 caaatcaaaa cgggacaaat cgaaaaagaa ttaagttttc aactattcgt tttgcgcaca
    25381 gggcagttgt taagaaatcg ataagaagaa agacgagggc aaagcatgcg ggaaggtcgt
    25441 ttgtttttgt cctttttttg acaaataaaa aaatcggccg aagccgattc ttttatttag
    25501 cgattttttt gcttaaacga gatttgtcgc ggttagcttt gtttttgtgg attagacctt
    25561 ttgattcagc catatcaata gctttaacag cttctttata taacgcatct acgttgtcag
    25621 cacctgaagc tacagcatct tcaaattttt tgattgcagt acgcatagcg cttgtttgag
    25681 atgaattttt cacattagcg ttttcgcttg tacgaacacg tttgattgca gattcaatgt
    25741 ttggcattag tttcacctcc gataacttaa gttcttacgc gtgttaccgc ataattcaac
    25801 attcattatt atacctaaag gcagcgtgct ttgcaatatg aacatgacaa gtttttttcc
    25861 ttaacagtct tttattagga ttttttttca gtaaaaacgg atagtacaag ctgaaaaaaa
    25921 tcaaaaagac gctattgtgg gaggcaatag cgtcaaacca aactagattt ttatttatct
    25981 atttaaacaa agtttgaatg ggaatgtttg gtactaagtt attatataac aaagagaacc
    26041 gtttgcaaag ttttttttga aaagggatac gattccctgt tgttctttgt ttttctatta
    26101 agaaactgca gttattttat aaccagtatc ctttgatcag taaaggctat tctggtgtac
    26161 cgattatttt aatgtattca tcgatatttt ttatcaacat tttttttatg accaactggt
    26221 tccgacggtt ccatttgcca cttacttcca gcacatattt atttaggggc aaacgtaaga
    26281 tatattttgc caaatagcga tctttaagca ggccattgat cacacggtcc tcaagctgca
    26341 agttgatccc agtcagcatc attggctgtt cttgtaaaac gtacgtatca attaaaagac
    26401 cttcaaaagt ttccacaagc gtcttccttt cttttcaaag taacctgaaa acaggcacta
    26461 attcttccat tttgatattc gttgaagtgg atgcgatcat tctttctttt gtccactgta
    26521 atctagtata acaaaaaaga gcagaagagc caaaaagaaa aaagagcatc gaattctata
    26581 ggaagtctca ttggcacggc ctaaattatt acaaaacatt gattaaaaaa agcccctgca
    26641 tcgcaggagc atcccctata tcgttttaat aggtcaatca aaaagcagta tcaaacacat
    26701 tttgtatcaa atcaggaaat aatagataag ctaagcccag aatcaaaagt accgctagaa
    26761 gaaaccagcc gattgttcct atgaaatact ttaatttttc gaacattgta tcacctcgtt
    26821 gtcagtatat cacggatcag taaattgggg gtgttaagaa tctcctaaaa tagacaaaat
    26881 ttgttctatt ttcctaaagc aaatacttgt aagatacagt aagtgatacc agaagcaatg
    26941 accggtccgg cggcgattcc tcttaagaag acaacaccta aaatcgtgcc gaaaacaagt
    27001 gcaacagtga tttgtggatc agaagcaatc aatcctacac ccttggaaga taagacagcc
    27061 actagcccac cgcaaaaaac agctacccag ccaatagggg atttaaaggc ttctaataga
    27121 tcacgtagac cgatttctcc tgttgcgata gggattagaa tagccgctgt gatgatcgtc
    27181 accccaatat tgattccttt gccttgaagc caagggaaaa aggaacggga ggctggtatg
    27241 agcttcataa tcaaaacaaa agctgccgct atcatcagac tttgattttt agctaaaatt
    27301 gctacaagta aaataattcc taaaaaaagc caactttcca tgaaatatat tcctcacttt
    27361 cttcgaaaca atcataacat gtttttagaa agttacaaaa gtaggaattc atccttcaag
    27421 acctattcat aagctgtttt tttcataaaa taaacaaaat cataaaaaaa cactttgctt
    27481 atttgacaga agacagccgc gctttgtata gtggacaaga cttacatttt tttgaagaat
    27541 cagaaagatc aggtaaaggt cttagtaaac gcaagccatt cagaataacc aatattgtgc
    27601 ttccttcgtg tcctacaact ccaagaggta gatttaaaaa ttgcaggaag ttagaagcaa
    27661 tcaacaagag aataacaaga atcgaaaaaa taatattttg tttgatgatc cgccgcattt
    27721 ttaaagatag ccgatgactg tagacaaatt tatttaagtc gcttttcatt aagacgatgt
    27781 ctgcgatatc catagcggca tctgtccctt gccccatagc gacacctaaa gaagcggctg
    27841 ctaaagcagg cgcatcattg actccatcac caaccatcgc atttacttgg taaagttcat
    27901 tctctttttt gactagttgt gttttttctt ccggagtaca gcttgcatag taatgttcaa
    27961 tccctacttg ttcagcaatg gcctttgctg tgccagaatg gtcgccggtt atcatacttg
    28021 tatggatgtt ttgggattta aagtaggaaa ttacttgttg ggtatttgct tttggaatat
    28081 ctagcagacc taaaacagca attaactgct gatctttaga aagataaatc acggttttgc
    28141 cttgattttc caaacgttcg atcatttccg caatctcttt gctgattatc atcgtctcag
    28201 gatcatatgc gtgcttgcca actttccatt gcgcacctaa ataggtagtt tgtaatccaa
    28261 aaccagtaat ttcttctatt ggtaagtatt ggatttcttc aggtattgtg caatcgaaat
    28321 attgcaaaat cgcatgagca agtggatgcg tcgttttttg ttccatagca acaagaagtt
    28381 gttgtgccaa atccttttca tcaaagaaaa agctgtcagt tacaactggt tttcctttag
    28441 tgagcgttcc tgttttatca aaggcaatcg cttttaattc agaaagcttt tctaaatgga
    28501 taccgccttt gattaaaatc ccattttttg cagaatttga aagagcagct agagaagctg
    28561 gtgtagcaga tgcgacaagc gcacaaggag aagccacaac taataatacc attcctcgat
    28621 aaaagctttc atcccaactc caacctagga aaaagtgggg gagaaggatc attagaggaa
    28681 cagcaagcaa aatcaatttt acataattat tttcgattcg ttcgataaat gaagctgttt
    28741 gagttggcgt attctgcgct tcttctacta attggataat cttagcaaag acagtttcgg
    28801 aactcgtttt tgttactcga atcgtgatgg gatttccttg attcagcgtt cctgcataca
    28861 tctcatcgcc taaatatttt tctacaggca cagattcacc gcttaatgca gactcatcga
    28921 tcgttgcctg tgtgctttct aaaaagccat ctatcggaac agctgctcct ttagcgacaa
    28981 ataccaagtt atccaccttt agttgagaaa catctacttc ttgcatttgt ccattttctt
    29041 ttaataattg ggctttttgt ggttgaagat tcatcaaagc agtaatttct ttttgacttt
    29101 tattagttgt gtattcctct aatgcaccgc ttaaacaaaa gatgaatgtc aacattgctc
    29161 cttcaaagta attaccgatc aaacaggcac caatagcagc taaagccatc aataaatcca
    29221 cattcagtgt ccgatcattc cacaattctt tcaagccttc cgatgtttgt ttatatccac
    29281 cgatgataat ggcactgata taaaaaagtg gggtgatggt aagagagaat cggtcgaaga
    29341 taaaaccgaa gatcataaat aaaaggcagc cgattgttgc tgcaaaagct tttttctgat
    29401 tagtcatcgt tattagacct ccttgtcaaa acaaggatag cataataaaa atgtaattga
    29461 taataataac agctaaatga gaacgataat ggttatcatt tggtgattaa gcttgatgtt
    29521 gtttttatta tttataatga ttattattaa agaaaataaa aaaccagaac atttctcaaa
    29581 taa
//
LOCUS       DACDKM010000022        27356 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20834_2016
            SAMN12640370-rid9891663.denovo.022, whole genome shotgun sequence.
ACCESSION   DACDKM010000022 DACDKM010000000
VERSION     DACDKM010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640370
            Sequence Read Archive: SRR10040891
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 27356)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27356)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742835.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742835.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:04:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,012
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 29 of 75
            Pseudo Genes (incomplete)         :: 33 of 75
            Pseudo Genes (internal stop)      :: 19 of 75
            Pseudo Genes (multiple problems)  :: 5 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27356
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640370-rid9891663.denovo.022"
                     /isolate="E20834_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(<1..690)
                     /locus_tag="IV334_000990"
     CDS             complement(<1..690)
                     /locus_tag="IV334_000990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HAP8943080.1"
                     /translation="MELSKRTKRLEPSVTLAAAAKAKALKAKGRDVLSLTVGEPDFAT
                     PENIQEAAIEAIRNGKASYYTPTAGIPELRQAIVDYLKKNYGLVYEPSQTVVTDGAKF
                     ALYTLFQTILDPQDEVIIPVPYWVSYGEQVKLAEGLPVFVKGEESNSWKVTVEQLEQA
                     RSEKTKAVIINSPSNPTGMIYSKNELQAIGEWAVKNDILIVADDIYGRLVYNGNEFTP
                     IATISEAIKNQT"
     gene            complement(711..1238)
                     /locus_tag="IV334_000991"
     CDS             complement(711..1238)
                     /locus_tag="IV334_000991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HAP8943081.1"
                     /translation="MKIKERRPLKKKQTKEKTINRVLLGLIVFLLAVIVISSIFYLRS
                     SRPAVQAKKEAVEIAKKYANLEETDHFYWFTRKKTYFSLTGTNDKGKEIAVIIPKSGE
                     KVKVLDQTKGLTENEAKQTIAAAHPEIQIEKAALGMYDDQPVWEVVGKETNGKLNYYL
                     IGFESGDEVKTIKGI"
     gene            complement(1285..4074)
                     /locus_tag="IV334_000992"
     CDS             complement(1285..4074)
                     /locus_tag="IV334_000992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HAP8943082.1"
                     /translation="MKPRTYAVVDIETTGTNPKEDRIIQFGCVMIESGRITSRFSIDI
                     HPGRKISKQIQHLTGITNQRVQKAPYFEDVAQTIYNLLADTIFVAHNIYFDYNFLNHE
                     LMRCGLPSLKIPGIDTVELAQIFLPTEPSFRLADLSESLGFIHENPHQADSDAEVTGQ
                     LLLLIEERMRKLPIITLEQIARLSRHTGMDTSRFIYHVIEEMKEKPEPLDPSLEIVDG
                     LALQKKEVELFTSVHYGERTYPRKKQAKEKMFGKTLMYRKEQNRLMNAVYDHFTKDES
                     KDLMIEAATGMGKTIGYLLPLSYLATPEKPAVISTVSLVLQQQIIEKDIPLLNQLLDQ
                     PIQPVIIKSYRHYIDLQRFKGTLVEPPEQKQYALYQMAVLVWLTQTKTGDLDELHLTN
                     LNHSFFADIAHRGIGFLARNQSFYEQDFVRFLQKKIRQSNFLIVNHAFLVQETQRKEP
                     LLPASPFLLIDEVHHLPEIAEKVNDKRLSINYFHKQIQQLEEPNQLFDRIQSYLPKEH
                     EALRWLSIYREELSALCEAQICMAEGLMSLVSGKQPNGQYPEEVMMTKEMLDQLPLES
                     GTAIDHLQLFYQEIQQLQSQMRLTFEIIQESWTNQMRIDLASLYSLFDQLDKQYLLMD
                     QWLEDWRDHLIHWFVPGNTAQQMIFHLHDFQAAMLPSTVWYPRYDRILYIGGTLKIGP
                     NRHYLSKRLGIEAAPLKVIASPYDYEKQARIYVPKEAPAVHQVSNQAYIDYLETALTG
                     LIEQEDRPILVLFTSHDVLQKVYQKMHLSMLEKGREILAQGFGGSREKLLKRFILSEK
                     SVLFGADSFWEGVDLPGDALQIVVVTRLPFDNPLRPMVKARNAYLESEGINPFYQESV
                     PKAALKLRQALGRLIRTETDRGVLLILDRRLVTAKYGARIIHALPKKVPIKELAMEEI
                     YQDIHEFLKKNEEGS"
     gene            complement(4221..4421)
                     /locus_tag="IV334_000993"
     CDS             complement(4221..4421)
                     /locus_tag="IV334_000993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HAP8943083.1"
                     /translation="METGTVKWFNSEKGFGFITAENGNDVFVHFSAIQGDGFKTLEEG
                     QAVTFDVEEGQRGPQATNVTKA"
     gene            complement(4874..5194)
                     /locus_tag="IV334_000994"
     CDS             complement(4874..5194)
                     /locus_tag="IV334_000994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8943084.1"
                     /translation="MKKRLLWCLLSLSLSLTSQPVFAEETQRFPITGTRLYKNVYVQT
                     PDLDSAIPETEERSLQPRFGYFWYCPTCDYESCWHFLHSTAAAFAFTHTQKYDGHHPV
                     IYEV"
     gene            complement(5697..6815)
                     /locus_tag="IV334_000995"
     CDS             complement(5697..6815)
                     /locus_tag="IV334_000995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8943085.1"
                     /translation="MAKKDKELLSQLTSEGAKGPFVTIMLNTHVAHQDVEKDQIKFKN
                     FAKEAKKRFEKKYSEHDWSPFQEKIENLLGDPSFWRSATTSVAVIITADQTYIHRLSI
                     PVDDQYYVSDLPYLLAIIKNGQFNYSYYLLGLNRDSMKLYEVSNKQVKEVSLPEGAPV
                     DVVTALGSELTGGGLNFSSNGGGGARDGVTFHGVNPKDEEVEIDWVNYYQAVDTYLKD
                     SFENPEQWPIYLFALPENQTMFKKIAKNPYYCADVAISASPAQLSFRDIEEGAKKLSS
                     ELAAQESKEYNKLLDKKFIDQLVDIVPAAKEGKVSHLFIATSNLADGFGEDPETEYDR
                     RQVLNALADDVIINGGKVFILDQKDAPDEKSLTAILRY"
     gene            7286..8593
                     /locus_tag="IV334_000996"
     CDS             7286..8593
                     /locus_tag="IV334_000996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HAP8943086.1"
                     /translation="MSIKKTRLVLLLASLILCLASFLPAVAVQAEDTFKVNAKAAFAV
                     DAQSGKILYDQDGEKTMGIASITKIIGLYIVLDQVKEGKLSWDDKVSISDYAENLSVT
                     PDLSNVPLHKENTYTVKELFDSAIIQSANASMVALAEKISGSEAKFTERMKEQLKDWG
                     IKDATIVNASGLNNSYLGENRPEGTGENDENQMSAQDVAIVARHLILDFPEILDVSST
                     TTQMFGENTQSPVEMVNWNWMLPGFVNYKEGVDGLKTGTTELAGACFVGTITKNDQRI
                     ITVVLNADGHAENPSARFNETARLMDYCYDNWSEKELGKANASIPSLKTIDVKDGKES
                     TVPVVLKSPVKVWVRNDMDTGKLTITPTLDKKQVTDNEIQAPMDKGTKIGTATITLAE
                     DKLGYLEDNNAPSTEIITDKTVEKANIFVIGWRRVAEFFGNLF"
     gene            8714..9913
                     /locus_tag="IV334_000997"
     CDS             8714..9913
                     /locus_tag="IV334_000997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HAP8943087.1"
                     /translation="MNLTRDLIFLVLYLIGVFSIVYWRKQKKDPSLFFYSSTWTFAVI
                     LTYFVVLEIFYSTFYFLIPLVFFSIFAFSYFSEKRRLLNGLLFNIFLISFGIYLFVLL
                     YETQNIFLGGLIALITIPLLLVFLFGIYGLIVFLFWNGVTVLRRESHSLANLLTLILA
                     IFLTLFLVFDFFLLKYLPQWINALFFCIPLILIYLFIVFYNFLTVSFLYQFNRPRYNQ
                     DFIVVLGAGLINGETVSPLLAKRINKAIAFYWAQSRATLNPPILLMSGGQGADEKVPE
                     AMAMKQYAMEQGIPERDILVETNSTTTLENMRYSKEIMDQQMKGPYRAIFSSNNYHIF
                     RAGLYARQAKLKANGIGAKTAFYYLPNAFLREYIAILMMNKKRHMIICGLLFAGSAFL
                     SLITLLF"
     gene            complement(9940..11208)
                     /locus_tag="IV334_000998"
     CDS             complement(9940..11208)
                     /locus_tag="IV334_000998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated chloride channel family protein"
                     /protein_id="HAP8943088.1"
                     /translation="MEKQLPSPVRILMYMLKWLVVTAILGVFMGSLSAFFLNSLTFVT
                     DIRLAHPWLLYLLPVSGALFAYLYAYHGGLSSRGNNLVIDQGNGGEEKIPLRLIPLTL
                     FGTITTHLFGGSVGREGTAVQMGGALADNIAHLFRLDKAEREILVISGISAGFSSVFG
                     TPLAGTLFGLEVLAIGKVRTEAIFPSFFAALFANFMTESYGVSHLHYQMGVIPEWSML
                     LFFKVFVAGIAFGLIGWVFSRSIVFLKARYAQWIPHPVWRNTIGGAVVVAAALILQTQ
                     RYLGLSLPLLQESFNGQAHAYDFIGKLFFTVLSLGAGYQGGEVTPLFEIGATLGAVLA
                     PLLHVSVPFLAGLGFIGVFSGATNTPIACFVMGIELFGSQAAAYFFMICLISFMCSGN
                     SGIYSAQQVAVKKGILFLPIVKRIKEKNKV"
     gene            complement(11378..12019)
                     /locus_tag="IV334_000999"
     CDS             complement(11378..12019)
                     /locus_tag="IV334_000999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010728800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G-binding protein"
                     /protein_id="HAP8943089.1"
                     /translation="MEQTMKPYQYFFIKREVEQLLNAYSSVNDSKTVQTVQALAAEKI
                     RDVLGYEPSEIDTFLTTVLDVKLTKTQAERALEELKQIVQPFMQPSKPQIEKLFRKVK
                     KLKQPAWNEIDLQAHTYIGWNDPGTQKKFMVYYEDGKLKGISGTLSPTINKGICAICQ
                     KTSNVSLFLSTTKAGSDGTYTKKGNYICHDSDQCNQQLTKLAPFYEFAERVKK"
     gene            complement(12428..12931)
                     /locus_tag="IV334_001000"
     CDS             complement(12428..12931)
                     /locus_tag="IV334_001000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HAP8943090.1"
                     /translation="MTEKWREDEEYMSYVEDLLETEAVKKLANYTQHVHSTRLEHSIS
                     VSYYSYLLAKKWGGNAKATARAGLLHDLFYYDWRTTKFDEGTHAYIHPRIAVKNAEKI
                     TDLSDLERDIILKHMWGATIAPPKYKEGYIVTFVDKYCAVKEAAQPMSASMRKRWQRY
                     FGKESSI"
     gene            complement(13054..13818)
                     /locus_tag="IV334_001001"
     CDS             complement(13054..13818)
                     /locus_tag="IV334_001001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HAP8943091.1"
                     /translation="MKEIQSAKNPRIKELKKLHKKKYREEKEEYLLEGFHLIEEAASS
                     DAEIKELFVTQRGMKEWGDWLAANKEISTFLVSDEVMDAVSDLPTPQGILAVMALTAE
                     GTPDFSGGWLLLDNVQDPGNVGTMIRTADAFDMAGVLLGKGTADIYSTKVLRSMQGSN
                     YHLPVLQKDLTEVIPLMKDKGFTVYGTELNEEAHALDKVEKSDNFAIIMGNEGQGVSQ
                     EILSLTDQNVYIPMKGNAESLNVGVAAGIVLYHFSL"
     gene            13926..14201
                     /locus_tag="IV334_001002"
     CDS             13926..14201
                     /locus_tag="IV334_001002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HAP8943092.1"
                     /translation="MRKVKMNVQGRVQGVGFRYMTKMVADQLGVTGTVKNEDDGSVTI
                     EAIGNDDIIQKFIEEVKKSPSPSGRVQYVDVQEDPMIEERKKFDVVG"
     gene            14761..15711
                     /gene="yidC"
                     /locus_tag="IV334_001003"
     CDS             14761..15711
                     /gene="yidC"
                     /locus_tag="IV334_001003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HAP8943093.1"
                     /translation="MNKWKNWLLGSGLVSILLFLSGCVRMDSNGNPDTSGIVYRVLVH
                     PMGQAITYLVENFNWSYGWAVIAMTVIVRIIILPLGISQSKKTMIQSEKMQALKPQVE
                     AAQQKLKAATTREEQMAAQAEMQQVYRENGLSMTGGIGCLPLLIQMPIFSALYFTARY
                     TEGIRESSFYGIDLGSPSMVLVVIAGVAYLLQGYISTIGIPEEQKKTMRTMLIVSPAM
                     IVFMSISAPAGVTLYWVVGGVFSCLQTFITNVMMKPRLKAQVAEELKQNPPKQVVTPR
                     KDVTESAKPANNKQATKQIKQPKNSTNNKGRNAGKQQKRK"
     gene            complement(15757..16158)
                     /locus_tag="IV334_001004"
     CDS             complement(15757..16158)
                     /locus_tag="IV334_001004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HAP8943094.1"
                     /translation="MSRQLVLISHGKFCEELKKSTEMIMGPQEDIYTLPLLPEDGTET
                     YKQKFLTVTDSLNDFVVLCDLLGGTPANIISKLIMEGQQITLYAGMNLPMVIEFINSQ
                     MIGSEPEFVRAGCESIVSVNELINGIAEEEE"
     gene            complement(16172..16981)
                     /locus_tag="IV334_001005"
     CDS             complement(16172..16981)
                     /locus_tag="IV334_001005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HAP8943095.1"
                     /translation="MTKSNYKLTKEDFKQINRRSLFTFQLGWNYERMQGSGYLYTILP
                     QLRKIYGDNTPELKEMMKTHTQFFNTSNFFNTIITGIDLAMEEKEGIASKETVSGLKA
                     GLMGSFAAIGDSVFAALIPTIFGALAANMATDGNPIGVFIWIFAQIVVMVFRWKQLEF
                     AYKEGISLVTTMQSRLTALTDAATLLGVFMVGALVATMINVKFSWAPTIGDVTLNMQN
                     YIDMILPKILPAGIVGGVYWLLGKKNMTPTRAIFIVLIVCVALSALGVISK"
     gene            complement(16968..17858)
                     /locus_tag="IV334_001006"
     CDS             complement(16968..17858)
                     /locus_tag="IV334_001006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAP8943096.1"
                     /translation="MDIQWWQILLLTLYAGYQILDELQIYSSMSAPVFAGLYAGLVMG
                     DLNAGLIIGGSMQLTVLGVGTFGGASKIDANSGTILATAFSISLGMNPEQAIAAIAVP
                     VASLMIQLDILARFANTYFAHRIDRHVEDMNYQAIERNFLMGALPWSLSRMIPVFLAL
                     AFGGGLVENVVSVLNGNLKWLGDGLSVAGAVLPAVGFAILLRYLPVKKHLSYLILGFT
                     ITALLATLFSNVQLLGTSVASVVKDLPATFNSLPMLAVALIGLALAMISYKNDQNKDH
                     QPLASEPSHSVEGEIEDDEI"
     gene            complement(17871..18350)
                     /locus_tag="IV334_001007"
                     /pseudo
     CDS             complement(17871..18350)
                     /locus_tag="IV334_001007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724290.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system
                     mannose/fructose/N-acetylgalactosamine-transporter subunit
                     IIB"
     gene            complement(18526..19467)
                     /locus_tag="IV334_001008"
     CDS             complement(18526..19467)
                     /locus_tag="IV334_001008"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexose kinase"
                     /protein_id="HAP8943097.1"
                     /translation="MITTVTLNPSIDIAYTIEELNLDKVNRVETVNKTAGGKGVNVAR
                     VLNLLGEEVIATGLIGGQHGEYLKNQLDQLGIMHQFVPIKGETRDSIAILHSSRQTEL
                     LERGPFITKKEQQTFTDHFEQLLEISDIMTISGSLPQGCPLDYYKQLVALADKKGKDI
                     LLDTSGKFLLEVLEGKEKPLLIKPNLEELGELIGQRICSSIQLEKIQSLLAHPIFEGV
                     PWVVVSLGERGAVAKVAQCFYEVIIPKISVVNPVGSGDATLAGSAHALSKRQSIEEIL
                     AFSMACGMANAQQEKTGWIDPMKVTMLMKHIQVKKIK"
     gene            complement(19464..20459)
                     /gene="lacD"
                     /locus_tag="IV334_001009"
     CDS             complement(19464..20459)
                     /gene="lacD"
                     /locus_tag="IV334_001009"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase"
                     /protein_id="HAP8943098.1"
                     /translation="MNEEKNRAMERLSTKDGIISALAIDQRGALKKMIKSLGKEPSED
                     QISEFKELVSAELTPYASAILLDPEYGLPAAKVRAKETGLLLAYEKTGYDATTPGRLP
                     DLLADWSSLRLKEQQADAVKFLLYYDVDEAPEINHLKHVFIERLGSECEGENRPFYLE
                     LVSYDAQISDSSSPEYAKVKPHKVIEMMKEFSDPKYKVDVLKVEVPVNMNYVNGYSTG
                     EFIHSKEEAAAFFKEQSEATPLPFIFLSGGVSTELFQQTLIFAHESGSTFNGVLCGRA
                     TWKDGVKAYVENGKDGAIHWLRTNGRENIETLNKVINETATPWFNKTKSKKVKMK"
     gene            complement(20452..21648)
                     /locus_tag="IV334_001010"
     CDS             complement(20452..21648)
                     /locus_tag="IV334_001010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HAP8943099.1"
                     /translation="MFELSKNKLKQIGAAITTQEIKQQPDLWKETIDRLMQSQENLAL
                     FFDQIKKEANGRRIRVIFTGAGTSQYIGDTLLPYLNEHGDNQFFIFHSFGTTDIVASP
                     KEFLFPNEPTLLVSFARSGNSPESVAAVQIANEQVETLFHLTITCAKEGMLAKNAQDD
                     KQNYLLLMPERANDAGFAMTGSFTCMLLSALWIFDQTAMEKKERYLEQLVKIGQDLLS
                     RDQEIQKLLPDFFERIAYLGSGSLAGATREAQLKILELTAGKVATIFDSSMGFRHGPK
                     SFLNSETLVVGFVSNQPYTRNYDLDILEEIKEDRVTANVIAIAQPGTIDFSGMTVLLE
                     DSVSKLPDAYLALAMIMTAQIIALYTSIKVANKPDTPSPTGTVNRVVKGVIIHKYETE
                     GEMTDE"
     gene            complement(21666..22394)
                     /locus_tag="IV334_001011"
     CDS             complement(21666..22394)
                     /locus_tag="IV334_001011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAP8943100.1"
                     /translation="MESSNKGKSLYYQLVDLLKDRIETQMTPHDKLLSERELCQHYGV
                     SRTTVRLALQELENQGYIYRRHGKGTYVSDLSDKSSNLAGAYSFTEEMREIGRVPKTV
                     ILEFRQMEANKRIAKNLNIHLGETVFKLKRLRLADEEPLMVERSYLPAKLFMGLDLPL
                     LESKPLYDVFSQDYEQQVQLADEEFYASIVQKKDAVVLRIADGAPVLHLTRITYNVKN
                     EIIEYTDSVARADQFHYKIRHIRK"
     gene            complement(22835..23809)
                     /gene="lacD"
                     /locus_tag="IV334_001012"
     CDS             complement(22835..23809)
                     /gene="lacD"
                     /locus_tag="IV334_001012"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase"
                     /protein_id="HAP8943101.1"
                     /translation="MKKMSIEKQERLKRLTDEAGIISALAIDQRGALKKMINQYKEAT
                     SEDIIQFKQMVSQYLTPYASAILLDPEYGLPAAALREKECGLLLAYEKTGYDAKMPGR
                     LPDLLNSWSVKRLKEVGADACKFLLYYDVDEPVEINVQKQIVIERIGSECEAEGIPFF
                     LELLSYDATIGDPSSLDYAKVKPHKVIEAMREFSKERYQVDVLKVEVPVNMRFVEGYG
                     QDTCYSQAEASAYFYEQSKVTEQPFIFLSAGVSAALFQDTLRFAKQSGSTFNGVLCGR
                     ATWAKGVAPFVTEGEEKASSWMLEEGKQNIKELSTLLEETATSIYVNK"
     gene            complement(23806..24264)
                     /locus_tag="IV334_001013"
     CDS             complement(23806..24264)
                     /locus_tag="IV334_001013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIA"
                     /protein_id="HAP8943102.1"
                     /translation="MTIIHEENIFLQQPLDNQEEAFKFLAKQTFCLGIAHDEKEVYQK
                     LLEREKEGTTGIMSGFAIPHAKSQTINEPSLIIVTLKKGIEWNSLDRQSVDFIFALFI
                     PDSEAGTTHLKLLSLVARSLMRKEVTEQLRAAKSKIEIATILTKEIGEDI"
     gene            complement(24261..25724)
                     /locus_tag="IV334_001014"
     CDS             complement(24261..25724)
                     /locus_tag="IV334_001014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP8943103.1"
                     /translation="MGKYQLIAATGCPTGIAHTYMAQEALEQAARKKGITIKVETHGQ
                     IGIENELTAEEIKQADAVIIAADKDVHSERFVGKRVIDVSVSTGIKDAQRLIDEALAG
                     KGTVLADNEAADTLEPEAISGANFGRSIYKNLMNGVSHMLPFVVAGGVLIAISFAVWG
                     IYSSDPDHAQYNATAAMVKAVDDAAMGMMVPVLAAYIAEGIAKRPGLVVGFVGGIVAM
                     NGGTGFLGGILAGFLAGYFILFLQKLLIILPKQLDGLKAIVLYPVIGVAFIGIVMSLL
                     AGPMKAINEGMMSFLAGFEYSNPLVLGVIVGCMCAFDMGGPVNKAAYVTGTALLTQGN
                     TTFMAGASAACIAPPLITGFATLLFGKYFDTNDRNAGLVNFILGSTHITEGAIPFAAK
                     DPIRVLPTMMLGSSIAAVLTYLFGVQVPAPHGGFLVLPVVTGKIQWVTAILIGSIVGG
                     VILGLIQKKRVTDKDKKRDNKATNRTVIEEGRAGGLS"
     gene            complement(25706..26638)
                     /gene="pfkB"
                     /locus_tag="IV334_001015"
     CDS             complement(25706..26638)
                     /gene="pfkB"
                     /locus_tag="IV334_001015"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010779385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HAP8943104.1"
                     /translation="MSIYTCTMNLAIDLFIETDEMHPFMVNRTKEDDIQANGKGVNVS
                     LVLKMLDFPSTALGFSAGFTGKYIEDYLQQKQIQTEFIEVPGMTRINVFTQVNQTGEE
                     YKLVNQGPEIPQTAVHNFLNQIRALQAGDYLCVSGSLPRGLSPKVLIEISRICLIIDS
                     SDQEVMDCLPYRPFLLKPNEEELSSWFGRKMVTDEDYFVYGQRLVESGAENVLLSLGE
                     KGAILFTKDRVIRGNSPKGKVVNTACAGDAMLGTFLAGYMNRRSLDETLRKSIAAGSS
                     TAFRKGLTDFLDVEELSKQIKIKEERFERWENIN"
     gene            26780..>27356
                     /locus_tag="IV334_001016"
     CDS             26780..>27356
                     /locus_tag="IV334_001016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HAP8943105.1"
                     /translation="MNHKLSETEQYLWNFIEHLILEIPNYSIVKLSEQANVSTATIVR
                     TMKKKGYEGFTSFKHHLKEGENKNINFSFLDKVDKGIRRAILKNEQEVVRTINMLEIG
                     NIEDAIQRIKFADRVFVFARGFSEMIGQEMLVKLQLTGKNCQMHTDPEIIKSISRKLT
                     KKDSVIFVSLNGEKTNKNIKNKIYVFIFQFFK"
BASE COUNT         8059 a         5879 c         4670 g         8748 t
ORIGIN      
        1 ggtctgattt ttgattgctt ctgaaatcgt tgcaattgga gtaaactcat tgccattata
       61 aactaaacgg ccataaatat catcagcaac tatcaaaata tcatttttta ctgcccattc
      121 accgattgcc tgcagttcat tcttgctata aatcattcca gtcggattcg atggagaatt
      181 gatgattacg gcttttgttt tttctgaacg tgcttgttct agctgttcaa cagtaacttt
      241 ccagctattg gattcttccc ctttcacaaa gacaggaagt ccttctgcca gttttacttg
      301 ctcaccgtaa ctgacccagt aaggcacagg aatgatgact tcatcttgcg ggtcaaggat
      361 cgtttggaaa agggtataca aagcaaattt cgctccgtct gttacgactg tttgtgaagg
      421 ttcatagaca agtccatagt ttttcttcag ataatcaacg attgcctgtc gaagctctgg
      481 gattcctgct gttggtgtat aatagctagc tttcccattg cggattgctt cgattgctgc
      541 ttcttggatg ttttctggtg tcgcaaaatc cggttctccc acagtcaaac tcagcacatc
      601 tctgcctttt gcttttaatg ccttggcctt tgccgcagct gccaaagtga cagacggttc
      661 taatcgtttt gttctttttg ataactccat cccattcatt cctttacctt ttaaattccc
      721 ttgattgtct tgacttcatc tccagattca aagccaatca ggtaataatt cagtttccca
      781 tttgtttctt ttcctactac ttcccacaca ggctgatcat catacatccc taaagcagct
      841 ttttcgattt ggatttcagg atgggccgca gcaattgtct gttttgcttc gttttcagtc
      901 agtccctttg tttgatccaa tacttttact ttttcaccag atttaggaat gataacggcg
      961 atttcttttc ctttatcatt tgtacctgtc agtgaaaagt atgtcttctt tcgagtaaac
     1021 caataaaaat gatcggtttc ctcaagattt gcatatttct ttgcgatttc tactgcttct
     1081 ttcttcgcct gcaccgccgg acgactggaa cgcaagtaaa aaatcgagct gatcacgatg
     1141 accgctaata aaaatacgat caagccaagt aacacgcgat taatcgtttt ttcctttgtc
     1201 tgttttttct tcaagggtcg tcgctccttt atcttcattc cttttcatta tagcagattt
     1261 taattatgaa aaaagacagc tcctctaact tccttcttca tttttcttta agaattcatg
     1321 gatatcctga tagatttcct ccatagctag ctctttgatc ggtacttttt tcggtaatgc
     1381 atggatgatc cgtgcgccat attttgcagt gactagtctt cgatccaaaa taagcaatac
     1441 gccacgatct gtctccgtcc ttatcaagcg acctaatgct tggcgtaact tcaaagctgc
     1501 tttaggtacg gattcctggt aaaaaggatt gatcccctct gattctaaat aagcgttcct
     1561 agctttgacc atcgggcgaa gcggattatc gaagggtaaa cgtgtgacta caacgatctg
     1621 caaagcatct ccaggcaaat ctacaccttc ccaaaagctg tcagcgccaa acaatacgct
     1681 tttttctgac aaaatgaagc gtttcaaaag cttctcccgg cttccaccaa acccttgagc
     1741 tagtatttct cgtccttttt ctagcataga cagatgcatt ttctgataga ccttctgcaa
     1801 aacatcatga gaagtgaata acacaagtat tggccgatct tcttgttcga tcagacctgt
     1861 aagtgctgtc tctaaataat cgatatatgc ttgattgctg acttgatgga cggcaggtgc
     1921 ctcttttggc acatagatcc ttgcctgttt ctcataatca taaggagagg caatcacttt
     1981 aagaggtgct gcttcaatgc caagcctctt agagagatag tgtcgattag ggccgatctt
     2041 cagcgttcct ccaatataaa gaatacgatc atatcgagga taccaaacag tcgacggcaa
     2101 catagccgct tgaaaatcat ggagatgaaa aatcatctgc tgcgctgtgt tcccaggaac
     2161 gaaccaatgg atcaaatgat ctcgccagtc ttccagccat tgatccatca gcaagtattg
     2221 cttgtctaat tgatcaaata ggctatataa acttgctaaa tcgatccgca tctggtttgt
     2281 ccagctttct tggataatct caaaagtcag acgcatttgt gactgtagct gctggatctc
     2341 ctgataaaaa agctgcaggt gatcaatggc tgtcccagat tccagtggca gctgatccaa
     2401 catctctttt gtcatcatga cttcttctgg atactgtccg ttaggttgct tgccgctcac
     2461 taaactcatt aatccttctg ccatacaaat ctgtgcttcg catagcgcag aaagttcttc
     2521 tcgataaatc gataaccagc gtaaggcttc atgttctttc ggtaaatagc tttgtattcg
     2581 gtcaaacagc tgatttggtt cttctaattg ctggatttgt ttatggaaat agttaatact
     2641 taatcgtttg tcatttactt tttcagcgat ttccggcaaa tgatgtactt catcgatcaa
     2701 caaaaatgga ctagctggaa gcaatggttc ttttcgctgt gtttcttgta ctaaaaacgc
     2761 atgattgaca atcaaaaaat tactttgcct gattttcttt tgcaagaaac gaacaaagtc
     2821 ttgttcgtaa aatgactgat ttcttgctaa aaatccaata cctctgtgcg cgatatctgc
     2881 aaagaatgaa tggttcaaat ttgttaaatg taattcatcc aaatcaccag tcttcgtttg
     2941 cgtcagccaa actaagaccg ccatttgata caaagcatat tgtttttgtt ctggcggttc
     3001 cacaagtgtg cctttaaaac gctgcaaatc aatatagtgg cgatagcttt tgataattac
     3061 aggttgtatg ggctgatcta gcaattgatt cagcaatgga atgtcttttt ctatgatctg
     3121 ctgctgcaaa actagcgaaa cggtactgat gacagcgggt ttttcaggtg tagccaaata
     3181 actcaatggc agcagataac cgattgtttt acccattcct gtagctgctt cgatcatcaa
     3241 atcttttgat tcatcttttg tgaaatggtc gtagaccgca ttcatcagtc tgttctgttc
     3301 ttttcgatac atcagtgttt taccaaacat tttttctttt gcttgttttt ttcttggata
     3361 tgtccgctcg ccataatgga cggatgtgaa tagttccact tcttttttct gcaaagctag
     3421 cccatcgacg atttctaatg agggatcaag tggctctggt ttttctttca tttcttcaat
     3481 cacatgataa atgaaacgac tcgtatccat acctgtgtgt ctacttaatc gcgcaatctg
     3541 ctctaacgtg atgattggca attttctcat ccgttcttcg atcagcaaaa gaagttgacc
     3601 tgtcacttcg gcatcactat ctgcctgatg aggattctca tgaataaatc caaggctttc
     3661 tgacaaatca gctaagcgaa agcttggttc cgtcggcaag aaaatttgtg caagttctac
     3721 tgtatcgatg cctggtatct tcaaactagg aagtccgcat cgcatgagtt catggttcaa
     3781 aaaattataa tcaaaataaa tattatgcgc gacaaatatg gtgtctgcca aaagattgta
     3841 aatcgtttgg gcgacatctt cgaaataagg cgctttttgg acgcgttgat tcgtgatccc
     3901 tgttaaatgc tggatttgtt ttgaaatctt ccgccctgga tgaatatcga tcgaaaaacg
     3961 tgaagtgatc cgacctgatt caatcatgac gcagccaaat tggatgatcc gatcttcttt
     4021 tggattcgtc ccagtcgttt cgatatcaac gactgcataa gttcttggtt tcaaaatatc
     4081 ttcctcttct cctgcctcta atgttctttt gaaattatac tacaaagtgc agaaaaatac
     4141 tctcatcgct ttttaaagaa aatagacaaa aacgcccaga cacgcaaatg tctgggcgtt
     4201 tattttttag tataggttaa ttaagcttta gtaacgtttg ttgcttgagg tccgcgttgg
     4261 ccttcttcaa catcgaaagt tactgcttgg ccttcttcta aagttttgaa tccgtcgcct
     4321 tggatagctg agaaatgaac gaatacatcg tttccgtttt ctgcagtgat gaatccaaat
     4381 cctttttctg agttaaacca ttttactgta cctgtttcca taataaaaat cctccttgtg
     4441 tatcaacaca tattttgcaa ttacttgaat ggtgtatatc tggaagatgt ttatttgaaa
     4501 caagctacca cagattaccg aacaaaaact actcctatag tatatcatac cgtttacaaa
     4561 aaagctagag tttttcgact ttttttatta aaaaaagttt tctcagcgat tacataaagt
     4621 gaaaaaccga actttttttt aaaataaacg acatattgtt cagttggtgt ttttttgctc
     4681 tgatgttctt ttttaaacta aaaaaattat tctttctgac tacttatcgg ttctgtttca
     4741 acggtcgagg ctggggaaga ttctttagta ggtctgatgt tctcatggta tttctgatag
     4801 cctatgacag ttaccagaag aacaaggcaa accaatacga ccaacgagat tagtttttct
     4861 tttttgctca tatctatacc tcataaataa caggatgatg tccatcatat ttctgagtat
     4921 gagtaaaagc aaaagctgca gctgtagaat gtaagaaatg ccagcaactt tcataatcac
     4981 aggtcggaca gtaccaaaag taaccaaatc ttggctgcaa agaccgttct tctgtttcag
     5041 gaatcgcaga atcaagatct ggtgtctgta cataaacatt tttgtacaga cgtgtgcctg
     5101 taataggaaa ccgctgtgtt tcttcagcaa aaactggctg acttgttaaa cttagggata
     5161 gagacagcaa acaccataac agtctttttt tcataaagac ctccttatcg ataagttttt
     5221 cccaattaaa atcatatctc aagaacgccc gttattcaca ttttgttcat aaatgatgtt
     5281 ttttgtatca taatcgtcaa aaagaggtcg tgaccacgtc acaacctctt ttttaaacgg
     5341 tgttccagaa gtagcttctt cggaaataag ccgaaatttc acaaaaattt gagaaataat
     5401 ttttgtcaat ctcttcttat ttcttgaagc tgaccacttc tgtcacaacc tcttccttaa
     5461 acggtgttca aaagcttgca tctgcggtaa taagcccacc aaacaaaaaa tatgaagagc
     5521 tatttttgct tgctgttctt attacttgat gcagaacagc tttttcacaa cctctcatac
     5581 acggtgttta atcagctgac cttcggtatt agctcaaaaa acggcaaaat atgtgaaact
     5641 atttttccgt ttgtttctct aatactcagg tcaaaacact gatttcacaa cctcttttaa
     5701 taacgaagaa tcgctgtcag actcttttca tctggcgcat ctttttgatc caaaataaac
     5761 acttttccgc cattaatgat cacatcatct gctaatgcat tcaacacttg acgccgatca
     5821 tattctgttt caggatcttc accgaaacca tcagctaagt ttgacgtagc gataaataaa
     5881 tgtgacactt ttccttcttt cgcagctgga acgatatcaa ctaactgatc gatgaatttc
     5941 ttatctaaga gtttattgta ttcttttgat tcttgagctg cgagttcact tgacagcttc
     6001 ttcgcccctt cttcgatatc tctaaaggat agctgcgcag gagaagcaga aatcgcaacg
     6061 tctgcacaat aatagggatt tttagcaatt tttttgaaca tcgtctgatt ttctggtaga
     6121 gcaaataaat aaatcggcca ttgttctgga ttttcgaaag aatccttcaa gtacgtatca
     6181 actgcttggt aatagttgac ccaatcgatc tctacttctt catcttttgg atttacgcca
     6241 tggaaagtta ctccatctcg ggctccgcct cctccattag aagagaagtt caaaccgcca
     6301 cctgtcagtt cagatcccaa tgcagtaacg acatcaaccg gtgccccttc tggtagagat
     6361 acttctttta cttgtttgtt gctgacttca tacaatttca tggagtcgcg gttcaatccc
     6421 aataaataat aagaatagtt gaactgccca tttttgataa tagccagtaa ataaggaaga
     6481 tcacttacat agtattgatc gtctaccggg atactcaaac gatggatata ggtttggtca
     6541 gcagttataa tgaccgctac actcgtagtt gcactacgcc aaaacgaagg atctcctaac
     6601 aaattttcaa ttttttcttg gaacggggac cagtcatgtt cggaatattt tttctcaaaa
     6661 cgtttctttg cttctttcgc aaaatttttg aatttgatct gatctttttc cacatcttga
     6721 tgggcgacat gcgtattcag catgatcgtg acgaatggtc ctttagcgcc ttctgaagtc
     6781 aattgtgaca ataattcttt atcctttttt gccataaacg tacccctcct aaaaaaatca
     6841 ttcctttgtt tctatctaaa gtctaccaca acactatttt tccatacaat aaaacgtatc
     6901 taaaaaaata aaacggtttc tacggtttta aatttagcta tttttttaag ttatcttcat
     6961 aatttcctca attatttagc cccagtcttc aactattctt catgaaatgc tgataaacta
     7021 agaaaaagaa taaaagaggt ggatcttatg catttgatta tgattttcag tttattaagt
     7081 attatcagtt tatttgcttt cagcgcttcc cgcttattag atgcggaaac acagcctatt
     7141 cctgtaaaag tacgtaaaaa cgaccgctaa ccttccataa cgtgcgttct tatcctaaag
     7201 ttttttcaga aacattgaaa ctctctcccc tctctgataa aattttaatt tgtagcgaaa
     7261 caaattaatt acaaggagat cggatatgtc cataaaaaaa acacgtctag tcctattatt
     7321 ggctagtctc attctttgcc ttgcttcttt tctaccagct gttgctgtgc aagctgaaga
     7381 tactttcaaa gtaaatgcaa aagcagcctt tgctgtagat gcgcaaagcg gtaagattct
     7441 ttatgatcaa gatggagaaa aaaccatggg gatcgcttca atcacaaaaa tcatcggtct
     7501 ttacatcgtc cttgatcaag taaaagaagg gaaactatca tgggatgata aagtttcaat
     7561 ttcagattat gcagaaaatt tgagtgtcac cccagattta tcaaatgttc ctttgcataa
     7621 agaaaacacc tacaccgtta aagaattatt cgactctgca atcatccagt cagccaacgc
     7681 ttcaatggtt gccttggcag aaaaaatctc tggcagcgaa gcaaaattca ctgaacgaat
     7741 gaaagaacaa ttgaaagatt ggggtatcaa agatgcaacg atcgttaatg catcaggact
     7801 gaataactct tatcttggcg aaaaccgacc agaaggtact ggagaaaacg acgaaaatca
     7861 aatgtctgct caagatgtgg ctatcgttgc ccgtcatttg atcttggatt ttccagaaat
     7921 actggatgta tctagcacta cgacacaaat gtttggcgaa aacacgcaat caccagtaga
     7981 aatggtcaac tggaactgga tgctcccagg attcgtgaat tataaagaag gcgtggacgg
     8041 attaaaaact ggtacgactg aacttgcagg agcctgtttc gtcggtacga tcactaaaaa
     8101 cgatcaacga atcatcactg tcgtattgaa tgcagacggc catgcggaaa acccaagtgc
     8161 ccgtttcaat gaaacagctc gcttaatgga ttattgctac gacaattggt cagaaaaaga
     8221 gcttggaaaa gccaatgcaa gtatccctag tctaaaaacc attgatgtaa aagacggaaa
     8281 agaatcaaca gtgcccgttg tgcttaaaag tcctgtaaaa gtatgggtac ggaacgatat
     8341 ggatactgga aaattaacaa tcacaccaac tcttgacaaa aaacaagtca ctgacaatga
     8401 gatccaagca cctatggata aaggaacaaa aatcggtacc gcaacgatca ccttggcaga
     8461 agataaatta ggttatcttg aagacaacaa cgcgccatcc acagaaatca tcacggataa
     8521 aaccgtagaa aaagcaaata ttttcgttat tggctggcga cgcgtcgcag aatttttcgg
     8581 taacttgttt taattgaaca aaaagaaaaa agcatctttc catgtggaag atgttttttt
     8641 gctttttcta gtttaaataa cagctttttt catctatttt cgttatactg tataagaaaa
     8701 aggagttgaa tttatgaatc taaccagaga tctgattttt ttggttctct atcttattgg
     8761 ggtattctcc atcgtttatt ggcgaaaaca gaaaaaggat ccttccttgt tcttttacag
     8821 cagtacatgg acgtttgcgg taatcttgac ttattttgtc gtgttggaaa ttttttattc
     8881 gaccttttat tttttgattc ccttagtgtt tttcagtatc tttgcctttt cctatttttc
     8941 agaaaagcga aggctgttga atggtctttt attcaatatt ttcctcatta gttttggtat
     9001 ctacttgttt gtcttgctat acgaaacaca aaatattttt cttggcggac tgattgcctt
     9061 gatcacgatc ccgttactgc ttgtctttct attcggcatt tatggattga tcgtttttct
     9121 tttttggaac ggtgtcacag ttttacgtag agagtcgcat tcattagcta atcttttaac
     9181 gttgatacta gccatttttc ttacattgtt tcttgttttt gactttttct tgttaaaata
     9241 tttaccacaa tggataaatg ctttgttttt ctgcatccca ttgatcttga tctatctatt
     9301 tatcgtattc tacaactttt tgactgtctc attcttgtat cagttcaacc gccctcgata
     9361 caaccaagac ttcatcgttg tgttaggcgc cgggttgatc aatggagaaa cagtttctcc
     9421 tcttcttgcc aagcgaatca ataaggcgat tgctttttat tgggcacaaa gccgtgcgac
     9481 attaaatccg cctatcctat taatgtcggg tggacaggga gcagacgaaa aagtaccaga
     9541 agcaatggcg atgaaacaat atgccatgga acaaggaatt cctgaaagag atattcttgt
     9601 cgaaaccaat tcgacaacca ctttggagaa tatgcgctat tcaaaggaaa tcatggatca
     9661 gcaaatgaaa ggtccctatc gcgcgatctt cagttccaac aattaccaca ttttcagagc
     9721 tggtctctat gcacgacaag cgaaactgaa ggcaaatggc attggggcaa aaacagcttt
     9781 ctattacttg cctaacgcgt ttttaagaga atacatcgcc atcttgatga tgaacaaaaa
     9841 aagacatatg atcatttgcg gattgctttt cgcaggttcc gcattcttat cactgatcac
     9901 tttacttttc taaaaaaaca aggtgtgaca aaacatatgt cacaccttgt ttttttcttt
     9961 tattctcttg acgattggca agaatagtat tccttttttg acggctactt gctgtgcaga
    10021 ataaataccg ctgtttcctg aacacataaa gctgatcaag cagatcataa agaagtaagc
    10081 agcagcttgt gaaccaaata attcgattcc catcacaaaa caggcaatcg gtgtatttgt
    10141 cgcaccagaa aataccccga taaaacctaa tccagcaaga aaaggaacag aaacatggag
    10201 caaaggcgct agtactgcgc caagcgttgc accaatctca aatagtggtg tcacttctcc
    10261 cccttggtac cccgcaccta aagacaagac cgtgaagaat aatttcccaa taaaatcata
    10321 agcatgtgcc tgtccattaa atgattcctg cagtaacgga agactcaacc caaggtaacg
    10381 ttgtgtttgc aaaatcaacg ctgcggctac aactactgct ccacctatcg tatttcgcca
    10441 aacaggatga ggaatccatt gcgcataccg cgcttttaaa aagacgatcg aacggctgaa
    10501 tacccaacca atcaaaccaa aagcaatccc agcaacaaaa actttgaaga acagcaacat
    10561 actccattca ggaatcacac ccatttggta atgtagatga gaaacaccat agctttctgt
    10621 catgaaattt gcaaaaagcg cagcaaagaa gctaggaaat attgcttctg ttcggacttt
    10681 accgattgcc aacacttcta atccaaataa tgttccagct aaaggtgtgc caaatacaga
    10741 actaaaaccc gcactgattc cactgataac caaaatctcc cgttctgctt tatccaatcg
    10801 gaataaatgc gctatattat cagctaatgc tccccccatt tgaactgctg ttccttcacg
    10861 accaacagat cccccaaata aatgggtagt gatggtccca aacaaggtca atggaatgag
    10921 ccgcaaaggg atcttctctt ctcctccatt cccttgatcg atcactaagt tattcccacg
    10981 actagaaaga ccgccatgat acgcataaag ataagcaaaa agcgcgccgc tgactggcaa
    11041 tagataaagt agccaaggat gtgctagtcg gatatctgtc acgaacgtca ggctatttaa
    11101 gaaaaaagca gacaagctcc ccatgaatac tccgagaatc gctgtgacca ccagccactt
    11161 caacatgtac atcaaaatac gcactggact cggtaattgt ttttccataa aaaattcctc
    11221 ctcaaataaa aaaacgccta ctaaagaaag gggctggccc cagactttag taggcgtcat
    11281 tagctatctt acgttagcat ttaaaggcga acaccatcac ctattcactt ccattaaaag
    11341 atagcatatt tttctgtaaa aaacaataaa attatcgtta tttctttaca cgttcagcaa
    11401 attcatagaa aggagccaat ttcgttaact gttgattaca ttgatcacta tcatgacaaa
    11461 tataattccc ctttttcgta tacgtgccat cagaaccggc tttcgttgta gaaagaaaga
    11521 gcgagacatt cgatgttttc tgacagatcg cacagattcc cttattaatc gttggtgata
    11581 aagtaccgct gatccctttt aattttccat cttcgtaata aaccatgaat tttttttgcg
    11641 tccctggatc attccaaccg atgtacgtat gtgcttgcaa gtcgatttca ttccaagcgg
    11701 gttgtttgag ttttttcact tttcggaata atttctcgat ttgtggtttg ctcggctgca
    11761 taaacggctg gacgatttgc ttcagctcct ccaatgctcg ttctgcttgc gtttttgtca
    11821 gttttacatc taaaactgtc gttaaaaacg tatcgatttc tgatggttcg taacctaata
    11881 catcgcgaat cttttcagct gctagagcct gaaccgtttg taccgttttt gagtcattga
    11941 cagatgagta cgcatttaat aactgctcta cttctctttt tataaaaaaa tattgatatg
    12001 gtttcattgt ttgttccatg attttaaagt cctttcttct aaccaaagtg catactttgt
    12061 agaaaaaagt gatcttctgc aaagtatgtg ttacctgaag atcccggact cttttaaata
    12121 agatacgttt tgcatcttat catcaccccc tttaatgttc attcttctat gtacatgatt
    12181 cattcgataa ataaaagtaa accgtatctt gagcagatag tcaactgcaa acagaaaaag
    12241 gactgtgaca aaagtcgtgt caaagtccct ttctacaaac aaacggtgtt ccagaagtag
    12301 cttcttcaaa aaataagccg gaatttcaca aaaatttgag aagcaatttt cgtaaattcc
    12361 ttcttatttc ttgaagctga cccacttctg tcacaacctc aatatggtct attattggtt
    12421 ttacctgtta aatcgaggat tcttttccga agtaacgttg ccatctctta cgcatacttg
    12481 ctgacatagg ctgagcggct tctttgactg cgcagtattt gtctacgaat gttacgatat
    12541 atccttcttt gtacttaggc ggtgcaatcg ttgcgcccca catgtgcttc aaaatgatat
    12601 ctcgctcaag atccgacaaa tcggtgatct tttctgcatt ctttacagcg attcttggat
    12661 ggatatacgc atgtgtgcct tcatcaaatt tcgtcgtacg ccaatcatag tagaacagat
    12721 catgcaaaag tccagcacgc gctgttgctt tagcattgcc accccatttt tttgcgagca
    12781 gataactata gtaagaaaca ctgatggaat gctccaaacg agtggaatga acatgctgtg
    12841 tgtaattcgc taattttttc actgcttctg tttctaaaag atcttctaca taagacatat
    12901 attcttcgtc ttcacgccat ttttctgtca taattatcga tctgccacct ttttgaattt
    12961 cattttcttc tgaaattgat ctaagtatac caccagtgtt cggattattc tagtcgcctt
    13021 ggataaaata gccaaagctt aatagttcct taatcataaa gaaaaatgat aaagaacaat
    13081 ccctgccgca accccaacat tcaacgattc cgcatttcct ttcatcggga tatatacgtt
    13141 ctgatcagtc aaagacaaga tttcctgact taccccctga ccttcgttcc ccatgattat
    13201 tgcgaagtta tctgattttt ctactttgtc taaggcgtgt gcttcttcat ttaattctgt
    13261 gccatacact gtaaaccctt tgtctttcat taaaggaatc acttctgtca aatctttttg
    13321 caacacaggc aaatgataat tgcttccctg catgctgcgc agcacctttg tactataaat
    13381 atctgccgtt cctttgccta ataaaacacc agccatatca aatgcatctg ccgtacggat
    13441 catcgtccct acatttccag gatcttgtac attatctaat aacagccatc ctcctgaaaa
    13501 atctggcgtg ccttcagccg ttaacgccat cactgctaag attccttgtg gtgtaggtag
    13561 atccgataca gcatccatga cctcatccga aactagaaaa gtagaaatct ctttgtttgc
    13621 ggccagccag tctccccatt ctttcatgcc tcgctgtgtc acaaaaagtt cttttatttc
    13681 tgcatcagac gacgcagctt cttcaatcag atggaaccct tccaaaagat attcttcctt
    13741 ttcttcccga tatttctttt tatgtagttt ttttaattct ttgattcttg gattttttgc
    13801 tgattggatc tctttcacat tcctcacctc cccatgatta tatcatggaa taggtgcttg
    13861 agcttgtttc atcctaaaaa aatcggtatc attaatttat aaaataggaa agaaaggtga
    13921 cgaacatgcg caaagttaaa atgaacgtcc aaggacgcgt ccaaggcgta ggatttcgct
    13981 acatgaccaa gatggtcgct gatcaactag gcgtgactgg aacggtaaaa aacgaagatg
    14041 acggctcagt cacgatcgaa gcaatcggca atgacgacat catccagaaa tttattgaag
    14101 aagtcaagaa atctccgagc ccaagtggac gagtgcagta tgtggatgtc caagaagacc
    14161 cgatgattga ggaacgaaag aaatttgatg tggtgggata aggttgtgaa aaagctggtc
    14221 tgcatcaagc attaaggaca ggttgtgaaa aatagctctc catatttttc aacaggctgg
    14281 acttaatgcc gtagatgcaa gcttttgaac accgtcaata aggttgtgag aaagtcgctt
    14341 agttttgagt agtaaggaga attttgtgaa aatggctttc cacatttttg cataaaattt
    14401 gacttaatac cgagaaactg acttttgaac accgtcgata aggttgtttt tgagacgctt
    14461 tgcctcaagt attaaagata ggtagtaaaa aagctgatct ataacttgca ataaatttgc
    14521 ttaacccata ttttccagac agaacaaaaa agtttttagt tttttaaata agaaagaaac
    14581 acttggaatc actcttttct ttccaagtat ttctttcttg attttttgtc tttttttcaa
    14641 aatttttcgc tacgtaaaag aacgaatatt tatatgaaat ttctatgaag ttgattgaat
    14701 tccatgcgct tttttagtat agttaagttt gtgtaaaaat tatagaagag gaagtattct
    14761 atgaataaat ggaaaaactg gttattggga tccggacttg tcagtatcct gctcttttta
    14821 tctggctgtg tgcggatgga ttcaaacgga aaccctgaca cttcgggaat cgtttatcga
    14881 gtactcgtcc atcctatggg ccaagcgatt acatatctag ttgagaattt caattggtct
    14941 tatggctggg cagttattgc aatgactgtg atcgtccgta tcatcatctt gccactaggt
    15001 attagtcaat cgaaaaaaac aatgatccaa tctgaaaaaa tgcaagcgtt aaaaccacaa
    15061 gttgaagcag cccaacaaaa attgaaagct gcaacaacac gtgaagaaca aatggcagca
    15121 caagcagaaa tgcagcaagt ataccgcgaa aacggattga gtatgaccgg cggaatcggc
    15181 tgtctgcctc tattgatcca aatgccgatc ttctctgcct tgtactttac ggcacgttat
    15241 acagaaggta ttcgggaatc atctttctac ggcatcgatt taggctcacc aagtatggta
    15301 cttgttgtca ttgcaggggt cgcctatctg cttcaaggat acatctcaac gatcggtatt
    15361 cctgaagaac agaaaaagac gatgcgtacg atgctaatcg tctctcctgc tatgatcgtc
    15421 ttcatgtcga tcagtgcgcc agccggtgtt acactttact gggtagtcgg tggtgtgttc
    15481 agctgtctgc aaacattcat cacaaacgtt atgatgaaac cacgtttgaa agcacaagtg
    15541 gcagaagagt tgaaacaaaa cccgccaaaa caagtcgtca ctccaagaaa agacgtgaca
    15601 gaatcagcaa agccagctaa caacaaacaa gcaacaaaac aaatcaaaca accaaaaaac
    15661 agtacaaaca acaaaggccg caacgcagga aagcagcaga agcggaaata aaaacatagg
    15721 acaaaaaaga tgagagctaa gccctcatct tttttgttac tcttcctctt ctgctattcc
    15781 attaattaat tcgttgacag agacaatgct ttcacatcct gcacgaacaa actcgggctc
    15841 tgaaccaatc atttgtgagt tgataaattc aatgaccatc ggaagattca tcccagcata
    15901 aagagtaatc tgttgtcctt ccattattag tttagaaatg atatttgcag gtgtaccacc
    15961 taaaagatcg catagtacca caaaatcatt taacgaatct gtgacagtga gaaacttttg
    16021 tttgtatgtt tcagtgccat cttctggtaa taaaggtaac gtgtagatat cctcttgagg
    16081 ccccatgatc atttctgtac tttttttcaa ttcctcacaa aattttccgt gactaatcaa
    16141 taccaattgt ctactcattt ttcgctctcc cttatttcga aatcacaccc aatgcagata
    16201 aagcaacaca cacaattaat acgataaaaa tcgctcgagt aggagtcatg tttttctttc
    16261 ctaataacca ataaacacca ccgacgatcc cagcaggtaa aattttaggt aagatcatat
    16321 cgatatagtt ttgcatattc aatgtcacat cgccaatcgt tggtgcccat gaaaatttaa
    16381 cattgatcat tgtcgcaact aatgccccta ccatgaatac ccctaataaa gtggctgcgt
    16441 ccgttaatgc agttaagcga gattgcatag tagtaactaa ggaaattcct tctttgtatg
    16501 caaattcaag ttgcttccaa cgaaaaacca tgacaacgat ctgagcaaaa atccatataa
    16561 acacacctat cggatttcca tcagttgcca tattcgccgc caaagcccca aaaatcgttg
    16621 gaatcaaagc agcaaatacg gaatctccaa tcgctgcaaa ggaacccatc aatccagctt
    16681 tcagccccga tacggtttcc tttgaagcaa ttccctcttt ttcttccatt gcaagatcaa
    16741 tcccagtaat gattgtatta aaaaaattag aagtattaaa aaattgcgta tgtgttttca
    16801 tcatctcttt taattctggt gtattgtctc catatatttt tcttagttgt ggcaagatag
    16861 tatataaata gccagaaccc tgcattctct catagttcca acctaattga aaagtgaaca
    16921 aacttcttcg attgatttgc ttaaaatctt cttttgttaa tttgtaatta gatttcgtca
    16981 tcttcgattt ccccctcaac agaatgtgat ggctcactag ctaatggttg atggtctttg
    17041 ttttgatcat tcttataact gatcattgct agtgctagac caatcaatgc tacagcaagc
    17101 atcggcagcg aattgaaagt tgccggaaga tctttaacaa cgcttgccac acttgttcct
    17161 aataattgaa cattagagaa taacgttgct aataatgccg tgatcgtaaa tcctaaaata
    17221 agataagata gatgtttttt tactggtaaa tagcgcagaa gaatggcaaa cccaacagct
    17281 ggtaaaacag ctcctgctac tgaaagtcca tcgcctagcc attttagatt cccgtttaaa
    17341 acagaaacaa cattttcaac taatccacca ccaaatgcta aagctaaaaa tacaggaatc
    17401 atacgagaga gcgaccaagg taaagctccc attaagaaat tgcgttcgat tgcttgataa
    17461 ttcatgtctt ctacgtgacg gtcaatacga tgggcaaagt atgtgttagc gaatctagct
    17521 aaaatatcca attggatcat taagcttgct acaggcactg caatagcagc aatcgcttgt
    17581 tctggattca ttccaagaga aatcgagaag gcagttgcta aaatcgtccc agagttggca
    17641 tctatttttg atgcgcctcc aaacgtcccc actcctaaaa ctgtcaactg catactgcca
    17701 ccaataatca aacctgcgtt taaatcaccc attactaaac ctgcatataa ccctgcaaac
    17761 actggcgcac tcattgaaga atagatctgt aattcatcta aaatttgata acctgcatat
    17821 aatgttaata ataagatctg ccaccattga atatccatgg atcattcctc ctatttgatt
    17881 aaaagattca taaagtcttc tgctttatcg tttggtacca tttgagcaat taaatgtata
    17941 ccaagctgat ctaatttttt aaatacttcg atatctgcat ctaccacatt gattgatttt
    18001 gtaatggacc gtgtttcatt cgtttgtgac atattcccca catttacttc tttgatagga
    18061 actccttttt ctactaaaga taagattcga tctggctttt ttgctacgat caatactcgt
    18121 tgagagtcat atttgccagc taaaatattt gttgctgctt tttcgattgg caaaatgctt
    18181 aattttaccc ctgctggagt cgctaacttc aatccacttt tttcaattgt gtttgtggct
    18241 acctcatcat ccacgaccat gattcgacta atattcagtt tcgttgtcca aagatttgct
    18301 acttatccat gtatcaaacg tccatcgatt cttacaccaa taattgccat catgcattct
    18361 ccttatctat tttttctatt tattgttcaa ttaaaactgt gacatcatca aatatccctt
    18421 ctgtttcaaa gataacgttt tcattttttc tttcttatat ttcattttta agctaagaac
    18481 aaattccttc ccaactaaag aaaacgcttt cttttatatt taagttcatt tgattttctt
    18541 aacttgaatg tgtttcatca acatcgtaac tttcataggg tcgatccagc cagttttctc
    18601 ttgttgcgcg tttgccatac cacaagccat agaaaatgcg agaatctctt cgattgattg
    18661 cctttttgat aacgcatggg ccgatcctgc aagggtcgcg tcacctgaac caacaggatt
    18721 aacaactgat attttaggaa taatgacttc gtaaaaacac tgagcaactt ttgccacagc
    18781 acctctctct cctaaagaaa caacaaccca tggcactcct tcaaatattg gatgagctaa
    18841 taaggactga attttttcta attgaatcga agaacagata cgttggccaa tcagttctcc
    18901 taattcttcc agattgggct tgataagtag tggcttctcc ttaccttcca atacttcaag
    18961 caaaaattta ccagaagtat ctagtaatat gtctttccct tttttatcag ctaaagcaac
    19021 caattgctta tagtaatcaa gagggcatcc ttggggtaaa ctacctgaga tcgtcataat
    19081 atctgatatt tcgaggagct gctcgaaatg atcggtaaat gtctgttgtt ctttctttgt
    19141 tatgaagggg cctcgttcca aaagctctgt ttgtctcgaa ctatgcagga tagcaataga
    19201 atcccttgtc tctcctttaa ttggaacaaa ctgatgcatg atccctaact gatctagctg
    19261 gttctttaga tattcaccgt gctggccacc tattaaacca gtagctatca cctcttctcc
    19321 taataaattt agaaccctcg ccacattcac tccttttcct cccgctgttt tattgactgt
    19381 ttcaactcga ttgactttgt cgaggtttaa ttcttcaatt gtatacgcga tatcgatgga
    19441 aggattcaag gtaactgtcg taatcatttc atcttcacct ttttgctttt tgttttattg
    19501 aaccaagggg tagcggtttc atttattact ttatttaaag tctcgatgtt ttctctccca
    19561 tttgttctta accaatgaat agctccgtct tttccatttt cgacgtatgc tttgacccca
    19621 tctttccatg tcgcacgtcc acacaataca ccattgaagg tggaacccga ttcatgagca
    19681 aaaatcagtg tttgttgaaa taattctgtt gaaacacccc cactcaaaaa aatgaatggt
    19741 aatggagtcg cttcactttg ctccttaaag aaggcagcag cctcttcttt gctgtgaata
    19801 aattcacctg tggaatagcc attcacataa ttcatattga ctggtacttc tactttcaac
    19861 acatcaactt tatattttgg atcagaaaat tctttcatca tctcaataac tttgtggggc
    19921 ttgactttcg catactctgg agaagaacta tcgcttattt gtgcatcata agaaactaac
    19981 tccaaataga agggacgatt ttctccttcg cactcactac ctaaacgttc gatgaacaca
    20041 tgcttcaaat gattgatttc gggtgcttca tcaacatcgt agtaaagtaa gaattttact
    20101 gcatccgctt gctgttcttt caatctgaga gaagaccaat ccgctaataa atcaggtaga
    20161 cgaccaggcg tagttgcgtc atagccggtt ttctcataag ctaacaataa ccccgtctct
    20221 tttgctctga cttttgctgc tggcaatcca tattccggat ccaataaaat agcagaagca
    20281 tagggtgtca gctcagctga tactagttct ttgaattcac tgatttggtc ttcacttggc
    20341 tcttttccca acgatttgat catttttttt agagcaccac gctgatcgat cgctaatgca
    20401 ctaataatcc catcttttgt tgatagtctc tccattgccc tatttttttc ttcattcatc
    20461 tgtcatttct ccttcggttt catacttatg aataatgacg cccttgacta ctcgattgac
    20521 cgtgcctgtt ggagatggtg tatctggttt attagctact ttgattgaag tatagagtgc
    20581 aatgatttgt gcagtcataa tcatcgctaa tgcaagatag gcatcgggca atttactcac
    20641 cgagtcttcc aatagcacag tcatacctga gaaatcaata gtacccggtt gcgcaatcgc
    20701 aatgacattg gcagtgaccc gatcttcttt tatttcctct aaaatatcta agtcataatt
    20761 tcgcgtatat ggttgattgc tcacaaaacc tacaacgagt gtctcgctat ttaaaaaaga
    20821 ctttggtcca tgacgaaacc ccatcgaaga atcaaaaatt gtagcaactt tacctgctgt
    20881 taattctaaa atttttaatt gtgcttctct tgtcgcacct gccaaacttc ctgaacccag
    20941 ataagcgatc cgttcaaaaa aatcaggtaa taacttttga atttcttgat ctcttgaaag
    21001 aagatcttga ccgattttaa ctaattgttc aagatatctt tcttttttct ccattgctgt
    21061 ttgatcaaag atccataaag cactcaataa catacacgta aaacttcctg tcatagcaaa
    21121 acctgcatcg tttgctctct ctggcatcaa taacagataa ttctgtttat catcctgtgc
    21181 gttttttgcc aacatcccct ctttagcaca agtaatcgtc aaatgaaaca atgtttcaac
    21241 ttgttcattt gcaatttgca cagccgctac actttctgga ctgttgccac tgcgagcaaa
    21301 tgaaactaat aaagtcggtt catttggaaa caaaaattct tttggtgaag ctacaatatc
    21361 tgtcgtgcca aaactgtgga agataaaaaa ctgattgtct ccatgttcat ttagataggg
    21421 taagagcgta tccccaatat attgagacgt tcctgcaccc gtaaaaataa cacgtattct
    21481 tcttccgttt gcttcttttt ttatttgatc aaaaaataga gctaaatttt cttggctttg
    21541 catcaatcta tcgattgttt ctttccagag atctggttgt tgttttattt cttgtgtcgt
    21601 gattgctgca cctatttgtt ttaatttgtt cttcgatagc tcaaacatgt tgttctccct
    21661 ctcaatcatt ttcggatatg ccgaatttta taatgaaact ggtccgctct tgctacacta
    21721 tctgtatatt cgataatctc atttttcacg ttataggtta tcctcgtcag atgtaagacg
    21781 ggagcgccat cagcgattcg tagcacaaca gcatcttttt tttgcacgat acttgcataa
    21841 aactcttcat cagctaattg aacttgttgt tcataatcct gtgaaaacac atcatacaac
    21901 ggtttacttt caagtaacgg caaatcaagt cccataaata gtttcgccgg tagataagaa
    21961 cgctcaacca tcaatggctc ttcatctgcc aaacgcaatc ttttaagttt aaatacagtt
    22021 tcacctaaat ggatgttcaa attcttagca attctcttat tagcttccat ttgtctaaat
    22081 tctaaaatga ccgtcttagg gacgcgccct atttcgcgca tttcttctgt aaagctatat
    22141 gctccagcta agttcgatga cttatcagac aaatcagaga cataggtacc ttttccatgt
    22201 cttcggtaaa tataaccttg attttccaat tcctgtaaag ccagacgtac ggtggtacga
    22261 ctcaccccat agtgttgaca aagttctctt tcagataaca gcttatcatg cggagtcatc
    22321 tgtgtttcga tacgatcctt caataaatca actagttgat aatacaatga ttttccttta
    22381 tttgagctct ccataaaaat cacctcttct ttactggtta taaccacaac acaagaatag
    22441 tgaattatta atcatttgtc aaaggttttt tagaccaaaa aagctattat aacagctctt
    22501 taaagcttta tatcttgaat agacaaagat acctcaaaaa taccagttac caataaaaga
    22561 acagaaaaaa agacgaacca cataagcgat ccgtcaaaat ggtctaactt atcgagcagc
    22621 gtgatccaca ttaatcccta tgatattcct acaactatcg ttttattact tccaatatca
    22681 tttacaacaa ctaaaaatag cttattaatg acaaatgtta ttattagttt tagataactt
    22741 ttgcagtttt atactttttt tctataaaat ttgacaaaca tttttttaaa taactaatta
    22801 ttttttgaat ctgtcactca gacaatcctt tctcttactt gttaacataa atagaagttg
    22861 ctgtttcttc taatagtgta cttaattctt taatattttg ttttccttct tctaacatcc
    22921 aactactcgc tttttcttcg ccttcggtaa caaaaggtgc tactcccttc gcccatgttg
    22981 ctcgcccaca taagacacca ttgaatgtcg aacctgattg ttttgcaaaa cgcaatgtat
    23041 cttgaaatag tgcagcgctg actcccgcac ttaaaaaaat aaacggttgt tcagtcactt
    23101 tcgattgttc atagaaatac gcggatgcct ctgcttgact ataacaagta tcttgcccat
    23161 atccttccac aaatctcata ttcactggta cttctacttt caatacatcc acttgataac
    23221 gttcttttga aaattctcgc atggcttcaa tcactttatg cggttttact tttgcatagt
    23281 caagagaaga cggatcgcca atagttgcat cgtaagaaag gagctctaaa aagaaaggta
    23341 tgccttctgc ttcgcattct gaaccaatcc gttctataac tatttgtttt tgcacattaa
    23401 tctctactgg ttcatcaacg tcatagtaca ataaaaattt acaagcatcc gcacccactt
    23461 cttttaacct tttcactgac caactattca gtaaatctgg tagtcggcct ggcattttcg
    23521 catcatagcc ggttttttca taagctagca acaaaccaca ttctttctct cgaagcgctg
    23581 ctgcaggcag accatattct ggatcgagta aaatagcgga agcatacgga gtcaggtact
    23641 gagaaaccat ctgtttaaat tggatgatat cttctgatgt agcttcttta tattggttga
    23701 tcattttctt caaagcacca cgttggtcga tagctagcgc gctaataatt ccagcttcat
    23761 ccgtcaatcg cttcaaacgt tcctgttttt ctatactcat tttcttcata tatcttcacc
    23821 tatttcttta gttaatattg tagctatttc tatttttgat tttgcagctc tcaactgttc
    23881 tgtaacctct tttcgcatca atgatcgtgc aacgagagac aataatttca aatgagtcgt
    23941 tcctgcttct gaatctggga taaataacgc aaaaataaaa tcaactgact gtctatctaa
    24001 actgttccat tcaattcctt ttttcagtgt gacgataatc aaactaggtt cattgattgt
    24061 ttgagacttc gcatgaggga tagcaaaacc tgacattatt cctgtagtac cttctttttc
    24121 tctttccaga agtttttgat atacttcttt ttcgtcatga gcgattccta aacagaatgt
    24181 ttgctttgct aaaaacttaa atgcttcttc ttgattatct aatggctgtt gtaaaaaaat
    24241 gttctcttca tgaatgattg tcatgataaa cctcctgctc ttccttcttc gataactgta
    24301 cgatttgttg ctttattatc cctcttcttg tctttatcag ttactctttt cttttggatc
    24361 aaacctagta tcactccacc aacgatcgaa ccaataagaa tcgctgtaac ccattggatt
    24421 tttcccgtca ctacaggtaa cactaaaaaa ccgccatgtg gggctggtac ttggacacca
    24481 aacagatagg tcaagacagc agcaattgat gagcctaaca tcatcgttgg taatactcgg
    24541 atcgggtctt tcgcagcaaa cggaatagct ccttccgtaa tatgagttga tcctaaaata
    24601 aaattaacca aacctgcatt acgatcatta gtatcaaaat atttaccaaa caataaagta
    24661 gcaaaaccag taatcaacgg tggtgcaatg caagctgctg acgcaccagc cataaaagtc
    24721 gtgtttcctt gtgtcaaaag agctgttcct gttacatatg ccgccttatt aaccggtcct
    24781 cccatatcaa acgcacacat acagccaaca atcacgccaa gaacaagtgg attcgaatat
    24841 tcaaatccag ctaaaaagct catcattcct tcatttatag ccttcattgg acctgccagc
    24901 aaagacatca cgatgccaat aaatgctaca ccaataactg gatataagac gattgccttc
    24961 aagccatcta gttgtttagg taaaattatc agtagctttt gcaaaaataa gataaaataa
    25021 cctgctaaga acccagctaa aatacctcct aaaaatccag taccgccatt catggctacg
    25081 attccaccaa caaatccaac gactaaccct ggacgtttcg cgatcccttc tgcaatataa
    25141 gctgctaaaa ctggaaccat cattcccatt gctgcatcgt caactgcttt taccattgcc
    25201 gctgtagcat tgtattgtgc atgatctgga tcagaagaat agattcccca aacggcaaat
    25261 gaaatagcaa tcaatactcc gccagctacc acgaaaggca acatatgaga gacaccgttc
    25321 atcaaatttt tatagatgct acgaccaaaa ttagcaccac ttatcgcttc tggttctaaa
    25381 gtatcagcag cttcattatc tgctaagaca gtaccttttc ctgccaatgc ttcgtcaatc
    25441 agccgttgtg catccttgat tcctgtactg acagaaacat caatgactcg ttttcccaca
    25501 aatctttctg aatggacatc tttgtctgcg gcaataatga cagcatctgc ctgtttgatt
    25561 tcttcagccg ttaattcatt ctcgatccca atttgtccat gggtctccac tttgatcgtg
    25621 atgccttttt tcctagctgc ttgctctaat gcctcttgcg ccatgtaggt gtgcgcaatt
    25681 cctgtcggac aaccggttgc tgcaattagt tgatattttc ccatctttcg aacctctctt
    25741 ccttgatttt gatttgttta cttaattcct ccacatccaa aaaatcagtc agtcctttac
    25801 gaaaagcagt agagctgcct gctgctatgc ttttacgtaa cgtttcatca agcgatcgtc
    25861 gattcatgta tcctgctaaa aaagtgccta acatggcatc acctgcacat gctgtattga
    25921 caacttttcc ttttggcgag tttcctcgaa tcaccctgtc tttcgtaaaa agaatcgctc
    25981 ctttctcacc gagggataac aaaacatttt ctgcgcctga ctctactagt cgttgtccat
    26041 aaacaaagta atcttcatct gtaaccattt ttcgtccgaa ccaagaagaa agttcttcct
    26101 catttggttt cagcaaaaac ggtcgatagg gtaaacaatc catgacttct tgatcactac
    26161 tatcaataat caaacaaatc cgactgattt ctattaaaac ctttggagac agccctcttg
    26221 gtaaactccc agaaacacaa agataatccc ctgcttgtaa tgctcttatt tgatttaaaa
    26281 aattatgaac agctgtctgc ggtatttcag gtccttgatt cactaatttg tattcttctc
    26341 ccgtttgatt gacttgtgta aatacattga tacgcgtcat accaggcact tcaataaact
    26401 ctgtttggat ttgtttctgc tgtaaatagt cttctatata ctttcctgtg aacccagcag
    26461 aaaaacctaa tgccgtactt gggaaatcta gcatctttaa tactagtgac acattcaccc
    26521 cttttccgtt tgcctgaatg tcatcctctt ttgtgcggtt taccataaaa ggatgcatct
    26581 catctgtttc aataaataga tcaattgcca aattcatcgt gcatgtgtag atagacataa
    26641 ccaacgacct ccttgattct attatgaaat acgcgaaata gaaaatgttt tcttaatcgt
    26701 cccaacacaa agaaaacctt tacatttata aactcgtcgt gcttttttta actaactttt
    26761 ggtgaaagaa ggaaagaata tgaatcacaa attaagtgaa acagagcagt acctctggaa
    26821 ctttatcgaa cacctcatcc tagagatccc taactattcc atcgtgaaac taagtgaaca
    26881 agcaaatgtc tcaacagcca ccattgtgcg gacaatgaaa aagaaaggct atgaagggtt
    26941 tacttcgttt aagcatcatc taaaagaagg agaaaacaag aacatcaatt tttcattttt
    27001 agataaagta gacaaaggaa tccgccgagc cattctaaaa aatgaacaag aagtggtacg
    27061 tacaatcaat atgttggaaa tcgggaacat tgaagatgca atacaaagaa tcaaatttgc
    27121 tgaccgggtt tttgtctttg cccgaggatt ttcagaaatg attggccaag aaatgctggt
    27181 aaaacttcaa ttaacgggaa aaaattgtca gatgcatact gatccagaaa ttatcaaaag
    27241 catcagtcgg aaactaacta agaaagacag cgttattttt gtttctttga acggtgagaa
    27301 aacaaataaa aacatcaaaa ataagattta tgtctttatt tttcaatttt ttaaat
//
LOCUS       DACDKM010000023        26310 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20834_2016
            SAMN12640370-rid9891663.denovo.023, whole genome shotgun sequence.
ACCESSION   DACDKM010000023 DACDKM010000000
VERSION     DACDKM010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640370
            Sequence Read Archive: SRR10040891
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 26310)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26310)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742835.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742835.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:04:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,012
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 29 of 75
            Pseudo Genes (incomplete)         :: 33 of 75
            Pseudo Genes (internal stop)      :: 19 of 75
            Pseudo Genes (multiple problems)  :: 5 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26310
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640370-rid9891663.denovo.023"
                     /isolate="E20834_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(1..606)
                     /locus_tag="IV334_001017"
     CDS             complement(1..606)
                     /locus_tag="IV334_001017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HAP8943106.1"
                     /translation="MNKKLAQYKRLLALSQAGLYYGKDVFDKERFEEIRTISLELISE
                     IGKETFEEIKALTTIGEGHPTPKVDVRAYIKKDGKVLLIEDKRTKEWSLPGGFAEIGL
                     SPEENVRKEVYEETGLTVETTQLRAVFDTNKQKDIPQLFQYYKLVFACAIHGEEAKFI
                     ENNETSNMGFFSIEELPKLSEKRTTKKQLLILENKNQIYCD"
     gene            complement(1056..1676)
                     /locus_tag="IV334_001018"
     CDS             complement(1056..1676)
                     /locus_tag="IV334_001018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAP8943107.1"
                     /translation="MKKYFILLAGSPGTGKTYLMNKLRERFPDMYAITLDEIKEYYAE
                     SIGFNNLEERAEQERTKVYPFYYKALDLYMEAGKKVIVSEYPFSDKQKARLQELADRY
                     GYQVITIRLTADFEVLCQRRYKRDRESDRHLSYIMDHYHYGDVLEDRTKGTNHITKEA
                     FKKIIADRKYDKFQLGTLYEFDVTDYTQVDYTPLLDKLEKEINDNK"
     gene            complement(1678..2547)
                     /locus_tag="IV334_001019"
     CDS             complement(1678..2547)
                     /locus_tag="IV334_001019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase family
                     protein"
                     /protein_id="HAP8943108.1"
                     /translation="MYTTLKEVTKTAEELNFTVGAFNTHNLEMLPAMLGAAKEMGSPI
                     IIQTSVDTAKYIGMHVLVNVMTAIAEEEMVDAVLHLDHARDFDDIKEAIDSGYTSVMY
                     DGSHLPFKENILKTKAVVEYAHAHGVSVEGELGTIGGTEEGIHVAENDKVYTRPEDAV
                     KFVEATGVDALAIAIGTNHGQFKSKTEVNIPLLKEIDAVVDVPLVIHGGTGVKEEDYP
                     ELINNGIRKFNVGTELLVNWTKVAKETFSETEINKSLRHNVIPANEAVKEIVKHKIGL
                     FMNLESPINLGNK"
     gene            complement(2570..2977)
                     /locus_tag="IV334_001020"
     CDS             complement(2570..2977)
                     /locus_tag="IV334_001020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HAP8943109.1"
                     /translation="MFGFFKKKEKEPKKVLAKEEKALSPEATEKIQSEITQLKQEIST
                     TQDKHKLAKLYEQVGLKFSELYVNDQAIQYLEKSLENKQTIGDGYKKLMSLYNQKRAD
                     AARAGDDQGIDYYMGKMDEMRQIAKQVTIKGNK"
     gene            complement(2996..4285)
                     /locus_tag="IV334_001021"
     CDS             complement(2996..4285)
                     /locus_tag="IV334_001021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018378547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAP8943110.1"
                     /translation="MGVINFIIENILTQASITIALIAMLGLLLQKKSAGQVISGTLKT
                     LLGFQVLSAGSSIIVGSLTYFGKIFTEGFHMQGIIPSIESINGQAMNDLGLGRDIALT
                     FLAIFVFNIILARFTKWKYIFLTGQAILWMATMTTVFGYFAGLRGIVLILVGGFIGAC
                     FAIAMPAVAQPIIRKITGSNDIALGHFCTIGYLFEAGVAKLFGEKGENKKSIEDIKLP
                     THFEFLQDTYLSVMVVMVPLYIITVLFAGEPFASELSGDQNYIMFAFLQAIQFVVGVY
                     VLLAGVRLLLGEIVPAFRGIAMKLVPDAIPALDCPVFFPYSPNAVILGFITTTIGTII
                     AMFTLPMFGLAMILPGMLTNFFAGGTAGIFGNAVGGRRGAIIGGIAHGFFITLLPALL
                     VTIFNSMGFINATATDVDTVAAALLYAWILSPVLKAF"
     gene            complement(4312..4614)
                     /locus_tag="IV334_001022"
     CDS             complement(4312..4614)
                     /locus_tag="IV334_001022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015509266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAP8943111.1"
                     /translation="MNKLNILFVCGAGLGSSFAAQMSAEDVLNKLGVEAKLDHCDISS
                     AVSMKPDVIITAENFRSQFEKFAVDPKTAMIYLKNIVSKNEIEEKITPVLQDKGVI"
     gene            complement(4639..6732)
                     /locus_tag="IV334_001023"
     CDS             complement(4639..6732)
                     /locus_tag="IV334_001023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HAP8943112.1"
                     /translation="MKTRTIEILHRIMTAQYPVKAKHLASEFNVSQRTIRQEVLEANI
                     WLSSKKLPEIRTIRNKVFLLKLEKEKQARLETEIKDVKDGFLNRRERIFDLVLAVAYS
                     RIPIHLNKKEEEFQISKSTLDEDMRRLRATLVSYGIEIVSYGKQGLVYKGSERSIRTM
                     IYDFINQNLGMTDFFMNQDAQLTPAQKIFLHYFPLVEIKKIQEIYSNKLLKQEDDIYK
                     KQILLFTLIWIQRIRKHEFISAINWKNTEIKDSSFSAFIDHMIEEFQLDNVPQVERNY
                     LRFTIETFNARDISNSIEWVQAQLLTIQLIQFVENETHVPFHLKEEALFENLYKHMAA
                     LIVRIKNHIQVMNPLKENIRTNYFPIYRAVTHFVPTIEEVIGGKILEDEIAFLVIHFS
                     TIASVIKRDLNYVYKSVVVCNHGMATGNLLAENLKEKFPQIEITAVLSSKEIELVDKL
                     DVNLVFSTFQLNYRNKPLLVVDPILTETNILVIEDFLKRNEELQRLEPNSHESTELFT
                     QIIDVMKESGGRVDRIIYGKLEKLFETNNLEINKREIQPMLKDILTDSNIMIKEKATT
                     WEESIELAARPLVKENIVEERYVEAMIRAVNDYGPYIVIGKHMALAHARPEDGVNKLG
                     VSVATIEQPIDFGNPEMDPVKIIFCLAAVDSYSHLNIMKELIELINNEEKLDRLIECT
                     NIEDFKQLLFSVVEK"
     gene            complement(7221..7985)
                     /locus_tag="IV334_001024"
     CDS             complement(7221..7985)
                     /locus_tag="IV334_001024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM44-like domain-containing protein"
                     /protein_id="HAP8943113.1"
                     /translation="MKKINYLAIILLVGLCLFALPTQGFAVAGGHGGGTSSGRSSGRG
                     SFGGRSYGGSYFGSRSYGRDSYGYYGPGTGPSSYEQTGFLALAVLSVGGVSAFSIVKN
                     KRQKKRLKAEILSRMSGTAKEKKAQFLEIQKIFMKIQSAWEEETPEKARSCYTDKLFA
                     EHQKVLQKNKADGVRNHTKKITIQEVGNYRQIHENSFAIRIDFRCCDYSVDRKDGRLI
                     SGSKTQRQYFSQIWYFDFSEKEKRWQADFIQPIMLD"
     gene            complement(8096..8965)
                     /gene="mdcB"
                     /locus_tag="IV334_001025"
     CDS             complement(8096..8965)
                     /gene="mdcB"
                     /locus_tag="IV334_001025"
                     /EC_number="2.4.2.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triphosphoribosyl-dephospho-CoA synthase MdcB"
                     /protein_id="HAP8943114.1"
                     /translation="MINDERKKAVTQQLAALVEEALIAEVTLSPKPGLVDALDAGAHS
                     DMDYALFLKSAKTLTPFFEEMAQIAWHHAIDQELRERIAEIGRKAEKAMLIATNGINT
                     HKGAIWAMGLLISVIAQKISMEQPFSLSKILDDVSKLAAFPDTKYRHKKTTHGHRVKQ
                     KYGVNGAYEEAILGYPHIRLAIQAAKELDNDSYVQKQMHMLLVLMSSLDDTCVLHRSD
                     QKTLVEMQKLAHAASQSALPNRPFTALLHFCQENQISPGGSADLLSASLFMLKIEELW
                     TADTYICPMLLND"
     gene            complement(8990..10066)
                     /locus_tag="IV334_001026"
     CDS             complement(8990..10066)
                     /locus_tag="IV334_001026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HAP8943115.1"
                     /translation="MFENKKIKINVSDFQKVSAVTAVMMQTILSFALANSHSATNTAF
                     LGTFYVIVKYTAPMFIFAIVYNMVKTSQHLSYWEFLKEKFFELVVPYLLWTTAYLWLF
                     PAVQQQTPYTDTASFLLKYVTGDGAPHLWYTVMMLQIQLFMPFFVWLGYKVFANKKYV
                     WPVLIGSTILYVAWYLFYQTQVLTGPYHDSWYLLDRFVASFMIYGVAAFVYHEKVYQY
                     LDRVRYLFLPVALVIAFFSVRSLLAHPGDLSFANAPYLNTIQSLYSLVIIFAVFIGAS
                     KMIVNDSPKLPLFKWLSVYAYRTYLANVFVFQVLLLLFKDSWLQLPSGVMILVAYLMT
                     ASCAFALSWLLHIIWVAIKKGFSK"
     gene            complement(10090..10683)
                     /locus_tag="IV334_001027"
     CDS             complement(10090..10683)
                     /locus_tag="IV334_001027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase holo-ACP synthase"
                     /protein_id="HAP8943116.1"
                     /translation="MIQAHDIVLFDKHEIEGFTLPEWVVLTEKMFGTVRRASTDHAEI
                     LPIGLRGPARNQRWSTEISKHAVKQVIHPWDIIEQESYRQEKIMDYPVYQQFHAARNI
                     LSGCKWGIGGSLGFELSTGIPAVKETSDFDLLLYANSPIELPIQVIQSHPAFFEQFDT
                     QVVTRQGGFSLKEYLREPEKKILLKTVEGPKLTKELW"
     gene            complement(10687..12363)
                     /gene="mdcD"
                     /locus_tag="IV334_001028"
     CDS             complement(10687..12363)
                     /gene="mdcD"
                     /locus_tag="IV334_001028"
                     /EC_number="4.1.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin-independent malonate decarboxylase
                     subunit beta"
                     /protein_id="HAP8943117.1"
                     /translation="MKNSFVELNARERVNALLDNGRELIDPFDQIIAPSLPAQGIVPE
                     SDDGIVVSRGTINGKQAVVIAMEGKFQGGGIGEVSGAKIVAALEHVLEENKKGNEIFP
                     VIVLDTGGVRLQEANYGLLSISEIGNVIIALKQYVPVIGLIPGRVGSFGGMSITSALM
                     SYLIATKKARVGLNGPEVIEQEAGVREFDSSDKDLIWNTLGSRQRVESKIIDELVTDS
                     VEAIKAAVIAAMDERKDTHRSESSAFYLSLLDSLDLSKPMAIDDYNEAIANHQAKSID
                     LPEVAEGNEPAAASVGYDWFAALTGMKNPTSSISTVYYGKSSKFIEEAIVTSIVPDPK
                     NPMYRVRNGEVGLVEGFRMGQILDHVYEEDKDKKVKRPIIVVIDVPSQAYGYNEELIG
                     IHVALANSAAAYAKLRQAGHPVIGLIAGNAISGAFLAHGLQSSRLIALNNKEITVQAM
                     SKESAARVTKRSVAEIEAAAEKVPAIAYDINNYTKLGAIYHFLEDITDQAASEQNVSK
                     VIEAINEAIQDVRNTNDTMLTNRYTNETAKTFGRVETNKVRAKMDEEWDA"
     gene            complement(12353..12652)
                     /locus_tag="IV334_001029"
     CDS             complement(12353..12652)
                     /locus_tag="IV334_001029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase subunit delta"
                     /protein_id="HAP8943118.1"
                     /translation="MEQLAYKYPTKQPINKKIHVGVVGSGDLEILMFPTEKTESTVKI
                     CTGSDGFGEVWNNVLTRFFDRYPISADIVINDFGATPGVVYLRLTQAMEELSDEK"
     gene            complement(12754..14400)
                     /gene="mdcA"
                     /locus_tag="IV334_001030"
     CDS             complement(12754..14400)
                     /gene="mdcA"
                     /locus_tag="IV334_001030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010748322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase subunit alpha"
                     /protein_id="HAP8943119.1"
                     /translation="MEKPNRNWASKREKKAQRLEKISNLLNGKYVETEKIVEVMEKLI
                     APGDKVVLEGDNQKQASFLSESLEKVDAEKVNDLHLIMSSISRPEHLNIFEKGIASKI
                     DFSYAGAQSLRVAQMIEDGTMKLGDIHTYLELYGRLFIDLIPNVVLVAADKADKDGNL
                     YTGFNTEETPTIIEAAAFKDGIVIVQVNEVVDSLPRVDIPGDWVDVVVKADKPYQLEA
                     LFTRDPQNITELQILMAMMAIKGIYAEHGVQSLNHGIGFNTAAIELLLPTYGESLGLK
                     GKIAKNWVLNPHPTMIPAIESGWVESIHSFGGEVGMEKYIAARPDVFFVGKDGTMRSN
                     RTLSQAAGQYAIDMFIGSTLQLDYEGNSSTVTKGRLSGFGGAPNMGHNPGGRRHSTPA
                     WQSLRDNDDPLGKGQKLVVQMVETYGSNKKPVFVEELDALAVQKQAGLANAPVMIYSE
                     DTTHIVTEEGIAYLYKAKTKEERQAAIAAIGGVTPLGMTSTKESLDSLRAAGIVKLPE
                     DLGIKRSEAKRSLLAAQTIDDLIEWSDGLYEPPMKFRTWS"
     gene            complement(14407..15318)
                     /locus_tag="IV334_001031"
     CDS             complement(14407..15318)
                     /locus_tag="IV334_001031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HAP8943120.1"
                     /translation="MSTGFLFPGQGSQKLGMLADIPEMYMERLENVTGYRLVDKEENY
                     QDTVFIQLALLTKAVFYLDEMEKNGIQPDIAAGHSIGAFCAAVACRSLRFENAAELVY
                     HRAVLMKEAYPSGYAMGVVVGVTRSEAEEIVAKTFDPDHPVYLSNENCPMQHTLSGSI
                     EGLSVTLAEAKRHQAQTAKLLKVPVPSHCLLMNDTVKKFKPYMQSVHLSDARCLYIKN
                     TDGRATQDAEEIRKDLLENLAFPVQWNQMMDLAKELGMDLTIEFPPGNTLTKLIHTKF
                     GVDKGIRTINLDQHGIDDATFLYQKWR"
     gene            15438..16295
                     /locus_tag="IV334_001032"
     CDS             15438..16295
                     /locus_tag="IV334_001032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAP8943121.1"
                     /translation="MKLLHLQYFKKVFETKNVTQAAKELFISQPALSRAIKHLENELG
                     VPLFYHSGRNIETTVYAEEFYPYAVKTLDTLEEGIKLLSSVNEKIVTSVILYLEVASV
                     SIPNLVRIFMEKHPDIQLTIMQHHLPDDITDPVLYITSEMKPGMTNIPIMKEPVYVAI
                     PKKHPLAKKEHLQLEDIQRTPILMLSKSNAFRHTLDAALEAKSIELTISSTTDDPATL
                     RSIVKQGLAVSFFPKVSWSYDKNDPFVLREIKDLPLTRTIYLSSSFTEDHPLIKMIAK
                     TMREFYLGK"
     gene            complement(16482..16844)
                     /locus_tag="IV334_001033"
     CDS             complement(16482..16844)
                     /locus_tag="IV334_001033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8943122.1"
                     /translation="MLTKKELIDHINETYNLSPEYIFKKFPDYCVFRHQKSKKWFGVL
                     MSVPEEKIYDDASDHRIINILDLKVDPELGGILRGKEGYFPAYHMNKEHWISIDLRKI
                     EKLDELADLIDGSFKSTQ"
     gene            17190..18629
                     /locus_tag="IV334_001034"
     CDS             17190..18629
                     /locus_tag="IV334_001034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HAP8943123.1"
                     /translation="MYSLMKKIITEKDIRRHVSLVEQLLNHTKITVNELAEIIGTTER
                     TIFSDLQSIRSQLPEGWDIISDQAGISLQNQQNLLTNDLWEIFFKQSVSVELLKNLLF
                     TKKVAVPDFLADHGLSYGTLKRHVTKINQRLASYDLQIDLTKYTACILGKERAIRTFY
                     HRLLIPFTHNNYFFEDYSIHESHYFQFLRNLSQTELAVETEEIFGTCWFFINTIRIKA
                     NCRLDSSIHTNSTLSSLYDSALKKLYLTEGIYLKDTELSFASFCFLESWNYNNNYGQE
                     TARCLHHSPFLEVLETFVEELASELSLDQLKKTSLTDNLALLILKYHESPILIEALDR
                     QYHRFLETYDQQYAHLYQQKENLLNDLKKEIEINEPNYFLQLLSLLIQQTIFSIKPNL
                     FNVYFIFQGEPSWKAFLQQELKDYLGKRVCFLPIELTDLSTISFEKTDILISNFPLDS
                     LDIPIVYISSIPTKNELNRLTELTFKTFL"
     gene            complement(18636..21188)
                     /locus_tag="IV334_001035"
     CDS             complement(18636..21188)
                     /locus_tag="IV334_001035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HAP8943124.1"
                     /translation="MNWYRLTVEDVLKNYQTSTEGLSQEERQKRLTDQGYNEIKARQQ
                     TKRWKKIAKHFTDLLMIVLIIASFLKFASSEYIEGSIILFVVIVNGLVSYWQERKAEE
                     SLNGLKQLMGQEAIVLSNGMQEKIPAKELVKGDVIVLRAGDVVPADIRLIEAHDLLVE
                     ESILTGESEASEKSHAVLTEEESIGDQKNMAFSGTLVQSGSAVGVVVETGNTTEIGKI
                     NQALQSVEQQTTPLIKKINRLNKQIFQGILCLSLFLVIFTTFRYGMDWHILLSATIAL
                     VVSMVPEGLPAVLTMILSVGVHEMAKEKAIIKGLPSVETLGSMTVICSDKTGTLTKNE
                     MTVVDVAAKEEACVLSIMKNCQELKTKEEQKTENLSGNPTEVALIRHTETANLPLKKI
                     EAKIPFSSEYKYMATMHAEEKGAVIYVKGAPEVLFAKSTLSSAEQEAWSQTAAEFARK
                     GQRVLGFAYKKVDSKQELTHETLTQLTFAGIAGLIDPPKESAVKAVKECQQAGISVKM
                     ITGDHKDTAKAIAEQIGLKHTAKVLEGIDLDLMSDEELIQQVPIVDVFARTTPEHKLR
                     IVKALQKNGEIVGMTGDGVNDAPALKRSDVGIAMGIKGSEVSKQAADMVLGDDNFHTI
                     AKAVKEGRRILDNLQKTINFFLPTALAQGLILIWALMLNRPLPLSPVQILWVNMVTTI
                     TLSYALGFESPDPEIMKRPPRDPKQGILSGYHLFRIIYVSLLIMVPAYLLAMQFEGQS
                     LQQTILLQNIVLAQAVYMINCRELSKPSLNQGLLQNKALFLSLGILALLQGMVIFLPV
                     GQQLIGTVSLTMTQQLLIGANVILLFLVVEIEKGIMNKLFRKKEKTAREYSR"
     gene            complement(21302..22633)
                     /locus_tag="IV334_001036"
     CDS             complement(21302..22633)
                     /locus_tag="IV334_001036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAP8943125.1"
                     /translation="MEKPAMKRTLTNRNIQLIALGGAIGTGLFLGSAKAIASAGPAVI
                     LVYLICGLAIYGLMRAMGDLFLANSTFNSISDFVDYYLGKKWAFFVNWTYWLCWLGAG
                     LAELTAIGLYIHFWFVHVPALISGGSALMILMAVNLVAVKWFGELESFFSIIKILGIF
                     LFILLGIGLSVSDFHTQSIGIKENFGQIDELLPFGFAGMVVSFPMVLFSFAGIEMIGL
                     TVGETSDPQKNLKKAINCLPWRILFFYIGTILSILFVVPWHCLDLKESPLVTMLQVIH
                     CQGGAAIVNLMILVASLSSANSAVYSTSRILYQTAKEKSLPHWFCALSKKGVPLHGIL
                     ITGMLFFAGLCLHFFVADSRLFFQLLAGMTTSCFLFVWSSILCAHVQFIKEKKRNTTQ
                     RYKDLALLVFFTSIAFSLLFESTTRFIPFLLVIWFVFLNFVYKRISNRNIS"
     gene            complement(22599..24077)
                     /locus_tag="IV334_001037"
     CDS             complement(22599..24077)
                     /locus_tag="IV334_001037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAP8943126.1"
                     /translation="MKDTGSSKQITTMTFIGMTCALVASVRNIPDVAATSWTMIFYML
                     VAVLLYAFPISLISGEFAGMFPQKGGPELWVSNALGKKWGFVVSWLLWVQMFPGMVMV
                     ASALAPLFGNIIDNVPLGLNSKFTLVVILVVYWIITLLNLKFDMAKIGGKVGVWLGLY
                     VPLTMMLLLGFAAWIKVGIVPTGTLGSFSWDKLIPDTTTASSFVYFAPIMFIFTGIEM
                     SSVYITRLENPVKTYIRGVFAALIFIFFVNILNALVVANVVPKGQMELNNIAQSISIY
                     CQILGLPHLIVNLFSLLVFIGVAVQLSAWASGPAKTVTESARKGAYPPKFNFWKTNQF
                     DVSKSVILTQSVIISIFALFYLLIPGVNQAFLMLVNSTTVIYCIVYVIMGIAVLRLRY
                     THAKLNRPFRIGKKEKKSNLGVWTVVIVLFAAIGFSVGLTMKAGTWINLIAVTAISVI
                     LFVVPLCIEKIKKPSWEQEVAMLLEKEKGELDGKTGNETDSN"
     gene            complement(24382..26217)
                     /gene="tdc"
                     /locus_tag="IV334_001038"
     CDS             complement(24382..26217)
                     /gene="tdc"
                     /locus_tag="IV334_001038"
                     /EC_number="4.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine decarboxylase"
                     /protein_id="HAP8943127.1"
                     /translation="MKDMDIKAVFIGDKAENGPVYKMLLNKMVDEHLGWRENYIPSDM
                     PAISEGDKLTPDYLATRDHMIEVLDEVSQRLRAGSIPWHSAGRYWGQMNAETLMPALL
                     AYNYAMLWNPNNVALESSMATSQMEAEVGQDFASLFNMTDGWGHIAADGSIANLEGLW
                     YARCIKSIPLAVKEVLPEKVKKMSEWELLNLSVEEILEMTESFTDEEMDEVKAASSRS
                     GKNIQRLGKWLVPQTKHYSWMKALDICGVGLDQMVAIPVQEDYRMDINALEKTIRELA
                     GQKIPILGVVAVVGTTEEGQVDSVDKIVQLRERLKDEGIYFYLHVDAAYGGYARSLFL
                     NEAGEFVPYASLAEFFEEHHVFHHCVTIDKEVYEGFRAISEADSVTIDPHKMGYVPYA
                     AGGIVIKHKNMRNIISYFAPYVFEKSVKAPDMLGAYILEGSKAGATAAAVWTAHRVLP
                     LNVTGYGQLIGASIEAAQRFREFLDHLTFTVKGKTIEVYPLNHPDFNMVNWVFKEQGC
                     TDLNAINELNEKMFDRSSYMDGDVYGERFITSHTTFTQEDYGDSPIRFVERMGLTKEE
                     WKKEQKITLLRAAIMTPYLNDDRIFNFYTKKIAKAMEKKLNEIIQ"
BASE COUNT         7616 a         5632 c         4513 g         8549 t
ORIGIN      
        1 tcaatcacaa tagatctggt ttttgttttc taaaataagt aactgtttct tcgttgttcg
       61 cttttctgat agctttggca actcttctat actaaaaaat cccatgttgg aagtttcatt
      121 attctcaata aacttggcct cttcaccgtg gatcgcacaa gcaaaaacaa gtttataata
      181 ctgaaaaagc tgtggaatat ctttttgttt attcgtatca aaaactgctc gcaactgagt
      241 agtttctact gttaatcctg tttcttcata gacttcttta cgcacatttt cttcaggaga
      301 caatcctatt tcagcaaatc caccaggaag tgaccattct ttcgtccgtt tatcttcgat
      361 tagtaggacc ttgccatctt ttttgatata ggcacgaaca tctacttttg gtgttggatg
      421 tccttctcca atcgttgtga gcgcttttat ttcttcgaaa gtttcttttc ctatttcact
      481 gatcagttcc aatgaaatcg tccggatctc ctcaaaacgc tctttatcaa atacatcttt
      541 tccataatat agaccagctt gagaaagtgc gagtagtctt ttgtattgag ccaacttctt
      601 gttcattctc ttcccttctt ttttagcaaa tgtatgacta tgtaatcaag tatagaagac
      661 tggtttaatt acggcaaata aatataatga aatctgcaat aaataaaaga atgttaaaaa
      721 actcgagaac aaaagacatt cgacttctct ggggctgtga ataaatggcg ttcaaaagct
      781 tgcatctgcg gtaataagcc ccaccaaacg aaaaatatga cgagctattc ttgtctggct
      841 gttcttatta attgaggcag gacagctttt tcacaacctc cccactaaac ggtgttctcc
      901 taccaactcc tcggtcacaa gtcacaatat tcatcaaaca tgagaagctg tttttttgaa
      961 attgctcctt gtgactcgga gctgaacgac aggctcacga cctctcggta aacagcgttc
     1021 aaaagccaat ttttcgatat taagccattt ctttgttact tattgtcatt gatttctttt
     1081 tctaatttat ccaatagtgg agtgtaatcc acttgagtat agtctgttac atcaaattca
     1141 tatagtgttc ctaactggaa tttgtcatat tttcggtcgg cgatgatctt tttgaatgct
     1201 tctttcgtaa tatgattggt ccctttcgtt cgatcttcca atacgtcacc atagtgataa
     1261 tgatccatga tatagctcaa atgacgatct gattcacgat cacgtttgta ccgacgttga
     1321 cataacactt caaagtctgc tgttaggcga atcgtgatca cctgatatcc gtatctatct
     1381 gctaattctt ggagtcgtgc tttttgctta tcactaaaag gatactcgga cacaatcact
     1441 ttctttcctg cttccatata caaatcaagt gctttgtaat agaaaggata aactttcgtc
     1501 ctttcttgtt ccgcccgttc ttccaaattg ttaaacccga tggattctgc atagtattcc
     1561 ttgatttcat cgagcgtgat ggcatacata tctggaaatc gttcccgtag tttattcata
     1621 agatatgttt ttcctgtacc aggactacca gccagaagaa tgaaatactt tttcatctca
     1681 tttatttcct aagttaattg gactttccaa gttcataaac agtccaatct tatgcttgac
     1741 aatttctttt actgcttcat ttgctggaat cacattatgg cgaagagact tgttgatttc
     1801 tgtttcacta aaggtttctt tagctacttt cgtccagttg actaaaagct ccgttccaac
     1861 attgaatttg cggatcccgt tattgatcaa ttcaggataa tcttcttctt tcacacctgt
     1921 tcctccatgg ataactaggg ggacatcaac tactgcatcg atttctttca gcaagggaat
     1981 gtttacttct gtttttgatt tgaattgtcc atgattcgta ccgatagcaa tagctaatgc
     2041 atccacgcct gtagcttcta caaatttcac tgcgtcttcc ggtcttgtat ataccttgtc
     2101 attttcagcg acatggattc cttcttctgt accgccgatt gttccaagtt ctccttctac
     2161 ggaaacaccg tgcgcatggg catattctac tactgctttt gtcttcagga tattttcttt
     2221 gaaaggcaga tgggacccat catacatgac agaagtatat ccgctgtcga ttgcttcttt
     2281 gatatcatca aagtctcggg cgtggtctaa gtgcaagact gcatcaacca tttcttcttc
     2341 tgcaatagct gtcattacat tgactaacac atgcatgccg atatatttag cggtatcgac
     2401 acttgtttga ataatgatcg gcgaacccat ttcttttgct gcgcctagca tagcaggaag
     2461 catttctagg ttatgcgtat taaaagctcc aactgtaaaa ttcaactctt ctgctgtttt
     2521 tgttacttct ttcaatgttg tatacatctt ttattgccac cttatctttt tatttgtttc
     2581 ctttgatcgt tacttgtttt gcaatttgtc gcatctcatc cattttgccc atgtagtaat
     2641 cgatcccttg atcatcacca gcacgagcag catctgcacg cttctggtta tagaggctca
     2701 tcagtttttt atacccatct ccgatcgtct gtttattttc caaactcttt tctaaatatt
     2761 ggatggcttg atcgttcaca tacaattcag aaaatttaag tccaacttgt tcataaagtt
     2821 tcgctaattt atgcttatct tgggtagtgg aaatctcttg tttcaattgt gtaatctctg
     2881 attgaatctt ttcggtcgct tctggcgata gggctttttc ttcttttgcc agaacttttt
     2941 tcggttcttt ttcttttttc ttaaaaaatc caaacatcat tttccctact ttcaattaaa
     3001 atgctttcaa aactgggctt aagatccagg catataagag tgctgcggct actgtatcta
     3061 catcggttgc tgtagcattg atgaatccca tcgaattaaa gatcgttact agcaaagcgg
     3121 gcaataatgt aataaagaaa ccatgagcaa taccgccaat gatcgctcct cggcgtccgc
     3181 caactgcgtt gccaaatatc cctgctgtcc ctccggcaaa gaaattggtt agcataccag
     3241 gtaaaatcat cgctaagccg aacatgggca aagtaaacat ggcaataatc gtaccaatcg
     3301 ttgtcgtaat gaaacctaaa atgacagcat ttgggctata aggaaagaaa actggacaat
     3361 ctaaggcagg gatcgcatca ggaactaatt tcatagctat ccctcgaaat gccggcacga
     3421 tttcacctaa cagtagacgt acaccagcta gcaatacata cacacccact acgaattgga
     3481 tggcttgtaa aaaggcaaac atgatatagt tttgatctcc tgataattca gaagcgaatg
     3541 gttcgcctgc aaataaaaca gtgatgatat atagcgggac catcacgacc atgacagaca
     3601 aataagtatc ttgcagaaat tcgaaatgtg tcggcaactt gatatcttcg atcgactttt
     3661 tattttcgcc tttctcacca aaaagcttag caacaccggc ttcaaataag tacccgatcg
     3721 tacaaaaatg acctaaagca atatcatttg atccagtgat tttacgaatg atcggttgcg
     3781 ctactgccgg catagcaatg gcgaaacaag ctccgataaa accaccgacc aagatcaata
     3841 cgatccctcg cagaccggca aaatagccaa agactgtcgt catcgttgcc atccataaaa
     3901 ttgcttgacc agttaaaaaa atgtacttcc attttgtgaa acgagccaaa ataatattga
     3961 atacaaaaat ggctaagaac gtcagtgcaa tatctctacc taaacccaaa tcattcattg
     4021 cttgaccatt gatagattca atagaaggga tgatcccttg catatgaaat ccttcagtaa
     4081 agattttccc gaaataagtt aaacttccaa cgatgatgct cgaaccggca ctcaacactt
     4141 gaaatcctag gagtgtcttt aacgtgccag agatgacttg tcctgctgat ttcttctgca
     4201 gtagtagacc caacatagca atcaatgcaa tggtgatcga tgcctgagtc aaaatatttt
     4261 cgatgataaa attgataacg cccatgtttt taccctcttt tctttttgta tttagataac
     4321 tcccttatct tgcagaactg gggtgatttt ttcttcaatt tcattttttg aaacaatatt
     4381 tttcaaatag atcatcgccg ttttaggatc aactgcgaac ttttcgaact gcgaacgaaa
     4441 attttctgct gtgatgatca catctggttt catggaaact gcggaagaaa tatcacaatg
     4501 gtccagtttg gcttctactc ctaatttgtt caaaacatcc tcagcagaca tctgtgctgc
     4561 aaaactactt cctaaacctg caccgcatac gaataaaatg ttcaatttgt tcatctttgt
     4621 tctcccccgt atcatttatt atttctcaac tactgaaaaa agcagttgtt tgaaatcttc
     4681 gatatttgtg cattcaatca atcgatccaa cttctcttcg ttgttgatca attcaattag
     4741 ttctttcatg atgttcagat gcgagtagga gtctactgct gccaaacaaa aaataatctt
     4801 tactggatcc atttcaggat taccgaaatc gattggttgc tcaatcgtag ctacgctgac
     4861 tcctaattta ttgaccccat cttctggtcg ggcatgggct agtgccatgt gtttgccaat
     4921 cacgatatac ggtccgtaat cattcactgc acgaatcatc gcttcgacat agcgctcttc
     4981 gacaatgttt tctttaacca atggtctagc agctagttca atcgattcct cccaagtagt
     5041 ggctttttct ttgatcatga tattgctgtc cgtcaaaata tcttttaaca taggctggat
     5101 ctcccttttg ttgatttcta aattgtttgt ttcgaataat ttttctaact tgccatagat
     5161 gattcgatct actcggccac cactttcttt catgacatct attatttgag tgaataactc
     5221 cgtcgattca tgactgttag gctctaaccg ctgtagttct tcatttcttt tcaaaaagtc
     5281 ttctataacc aagatattcg tctcagttaa aatcggatcg acaactaaaa gcggcttatt
     5341 tcgataattc aattgaaagg tgctgaaaac cagattgaca tccaatttat ctactaattc
     5401 gatttctttt gaacttaaaa ctgcagtaat ctcgatctga ggaaactttt ctttcagatt
     5461 ctctgctaac agatttccag tagccatccc atggttacag acaacgactg atttataaac
     5521 ataattcaag tctcgcttga ttacactggc gatcgtcgaa aagtggatga caagaaaagc
     5581 tatctcgtcc tccagtatct tgccaccaat tacttcttcg atcgtaggaa caaaatgagt
     5641 gactgcgcgg tagattggga aataatttgt tcggatgttt tctttcaatg gattcatgac
     5701 ttggatatga ttcttgattc ttacaatcag tgcagccatg tgcttataga gattttcgaa
     5761 taaagcttct tcctttagat ggaaaggtac gtgtgtctcg ttctctacaa attggatcaa
     5821 ttggatcgtt agtaattgag cttgtaccca ctcgattgaa ttggagatat ctctagcatt
     5881 gaacgtctca atagtgaaac gaagataatt tctttctact tgcgggacat tgtccaattg
     5941 aaattcctcg atcatgtggt caataaatgc agaaaagcta ctgtctttta tttctgtatt
     6001 tttccaatta atggcagaga taaattcatg tttcctgatt cgctggatcc agatcaatgt
     6061 aaaaagcagg atttgttttt tataaatatc atcttcttgt ttcaacaact tattggaata
     6121 gatctcctgt atttttttga tttccaccaa aggaaaataa tggagaaaga ttttttgagc
     6181 cggtgttaat tgtgcatctt gattcataaa gaagtcggtc attcccagat tctgattgat
     6241 aaaatcatag atcatcgtac ggatagatcg ttctgaacct ttataaacca gtccttgctt
     6301 tccataactg acgatctcta tcccataact gaccagtgtt gctcgtaacc gtctcatatc
     6361 ttcatctaat gtacttttag agatttgaaa ttcttcttct tttttgttta gatggatcgg
     6421 gattcggctg taagcaacgg ctaaaactag atcaaaaata cgttcgcgcc gattcaaaaa
     6481 gccgtctttc acatctttta tttcagtttc taatcgagct tgcttttcct tttccagctt
     6541 caacaaaaaa actttgttgc gaattgtacg gatctccgga agctttttac tgctcaacca
     6601 gatattcgct tcaagaactt cctgacggat cgtccgctga ctgacattga actcacttgc
     6661 tagatgtttt gcttttactg gatattgggc tgtcatgatt ctgtgaagta tttcaattgt
     6721 tcttgttttc atagaaatga tctccttctg atttcaagta tatggttccg cttacataat
     6781 aaaaagtaga tatttctgcc acttgaagtg gaaagaaagt aagcaaagca aacaaaaaat
     6841 aagccatatg ctttttcatc tcctcaacga aaaagacata cgcctattat tacaaaaatt
     6901 ttgcccatct atacaaaaaa gaggttgtga aaaagctgtc ctgcatcaag taataagaac
     6961 aactaaacta aaatagtgct tcctattttt cgtttggttg ttcttattat cgcagatgca
     7021 agcttttaaa caccatttag tggagaggtt gtgacagaga tgttcagcct tgagcgataa
     7081 gaagcgattt tgaaaaatcg cttcccatat tttacaaaat cgtgccttat tgccgatagg
     7141 ctatctctgg cacactgttt attcagaaaa aggagctgcg acaaggcttt tgtcacaact
     7201 ccgttcataa atatttcctg tcaatccagc ataatcggct gaataaaatc ggcttgccat
     7261 cttttttctt tctcagaaaa atcaaaatac catatctgag aaaaatactg tcgttgtgtc
     7321 ttacttcctg agatcaatcg cccatctttt cgatcgactg aataatcaca gcatctgaaa
     7381 tcaattcgga tggcaaaact attttcgtgg atctgtcgat aatttcctac ttcctgaata
     7441 gttatttttt tagtatgatt tcttacaccg tctgctttgt ttttttgcag aactttctga
     7501 tgttcggcaa acagcttgtc tgtataacag cttctggctt tttcaggagt ttcttcttcc
     7561 caagcggatt ggatcttcat gaatatcttt tggatttcca gaaactgtgc tttcttttct
     7621 ttagccgttc ctgacatgcg ggacaatatc tccgctttca gccttttctt ttgtctttta
     7681 ttcttgacga tagaaaaagc agaaacccct cctactgaca aaacagctaa tgcaagaaat
     7741 cccgtttgct cataactaga aggacctgta cctggaccat aatacccata agaatctctg
     7801 ccgtaagaac gactgccaaa ataactgccc ccatatgaac gaccaccaaa tgaacctctg
     7861 cccgaacttc gaccgctaga tgtacctcca ccatgaccac ccgcgacagc gaatccttgg
     7921 gtcggcagag caaacaaaca taggcccact aacaaaatga ttgctaaata attgattttt
     7981 ttcattgtct tcctcgttat cttttattac aaaaaactat ttttatgata gccttgctaa
     8041 aaggaagagt caataaccag atgcataaac tgttttttta gcaattttgt cttatttagt
     8101 cattgagtaa catcggacaa atgtatgtgt cggcagtcca taattcctct atcttcagca
     8161 tgaacaagct tgcagacaag agatctgcgc ttcctccagg agagatttgg ttttcctgac
     8221 aaaagtgcaa cagtgctgta aatggacgat ttggtaaagc actttgacta gcagcatgag
     8281 caagtttttg catttccact aacgttttct gatcactgcg atgcaatacg catgtgtcat
     8341 ctagcgaact catcaaaact aacaacatat gcatttgttt ctgtacatat gaatcattat
     8401 ctagctcctt agctgcttgg attgcaagcc tgatatgcgg ataccctaat atcgcttctt
     8461 catatgcacc atttacgcca tatttttgct tcaccctatg accatgagtg gtttttttat
     8521 gacgatattt tgtatcagga aaagcagcaa gcttgctgac atcatcaagt attttcgata
     8581 aagaaaaagg ctgttccata ctgatttttt gtgcaatgac actgatcaat agccccattg
     8641 cccaaatcgc tcctttatgg gtattgatgc catttgtagc aatcagcatt gccttttccg
     8701 cttttcgccc aatctcagca attctttctc ttaactcctg atcaatagca tgatgccatg
     8761 ctatttgagc catttcttca aaaaaaggag ttagcgtctt agcagactta agaaataaag
     8821 catagtccat atcggaatgt gcacccgcat ccagtgcatc cactaatcca ggttttggac
     8881 ttaatgttac ttctgcgatc aatgcttcct ctaccaacgc agcgagttgc tgagtcactg
     8941 ctttttttcg ttcgtcattt atcatgacac tcactccttc ttcctactat tacttgctga
     9001 aacctttttt gatagctacc caaatgatat gaagtaacca gcttaaagca aatgcacaac
     9061 ttgccgtcat tagataagct acaagaatca tgactcctga aggtaactgc agccaactat
     9121 ctttaaacaa taataacagt acttggaaaa caaatacatt ggctaaatac gtacgataag
     9181 cataaacaga caaccatttg aacaatggca attttggcga atcgtttaca atcattttgg
     9241 aagcgcctat gaagacagca aagatgatga ctaaactgta taaactttgg atcgtattca
     9301 agtaaggtgc attcgcaaag ctcaagtctc ctggatgtgc gagcaatgaa cggacagaaa
     9361 agaacgcgat aactaaggca acaggtaaga aaagataacg tactctgtcc aaatattgat
     9421 agactttttc gtgataaaca aatgcggcta ccccataaat catgaaacta gcaacaaacc
     9481 gatctaataa gtaccagctg tcatgataag gcccagtcaa tacttgtgtt tgatagaaca
     9541 gataccatgc gacatataaa atcgttgaac caatcaatac tggccataca tattttttat
     9601 tagcaaatac tttgtaaccg agccaaacaa aaaacggcat gaacaattgg atctgcagca
     9661 tcataacggt ataccataaa tgaggcgctc catcacctgt gacatatttc agtaaaaagc
     9721 ttgcagtatc cgtatacgga gtttgttgtt ggactgctgg aaatagccaa agataagccg
     9781 tcgtccatag aagatacgga acgactagtt caaaaaactt ttctttcaaa aattcccaat
     9841 aagacaaatg ctgacttgtt tttaccatat tataaacaat tgcaaaaata aacattggtg
     9901 ctgtgtattt aacgatgacg taaaaggtac caagaaaagc tgtatttgtt gcgctgtggc
     9961 tgtttgccaa agcaaagctc aatatcgttt gcatcataac agccgttaca gctgatactt
    10021 tctggaagtc acttacgttg attttgattt ttttgttttc aaacatctct ttctctctct
    10081 ttttctttat taccaaagtt ctttggttaa tttaggtcct tcgactgttt tcaatagaat
    10141 ttttttctct ggctctctca aatactcttt tagtgaaaaa ccgccttgcc gagtcactac
    10201 ttgggtatcg aattgctcaa aaaaagcagg atgcgattga attacctgaa ttggcaattc
    10261 aatcggtgaa tttgcgtaca gcagcaaatc aaaatcactg gtttctttta ctgccggaat
    10321 cccagtcgat aattcaaatc ctaagctgcc gccgattccc catttacatc ctgataatat
    10381 gtttctagca gcatgaaatt gctgataaac aggataatcc atgattttct cttgtctata
    10441 cgattcttgt tcgatgatgt cccacggatg aatgacttgt ttgacggcat gttttgagat
    10501 ttctgttgac catctctgat tgcgggcagg tccacgtaaa ccaatcggta gtatttctgc
    10561 gtgatccgtc gacgcacgac gaactgttcc gaacatcttt tcagttagaa cgacccattc
    10621 aggcaaggtg aacccttcta tttcatgttt atcaaaaagg acaatatcat gtgcttgaat
    10681 cattttttat gcatcccatt cttcgtccat tttggcacgg actttatttg tttcgacacg
    10741 tccgaatgtt ttggctgttt cgtttgtgta tcgatttgtc agcatcgtgt catttgtatt
    10801 gcggacatct tgaatggctt cattgattgc ttcgatgact ttgctgacat tttgttctga
    10861 agcagcttgg tcggtgatat cttctaggaa atgatagatc gctcctagtt ttgtataatt
    10921 attgatatcg taagcgatag ctggaacttt ttcagcagct gcttcgattt cagcaaccga
    10981 acgttttgtc acacgtgcag cactttcttt agacatcgct tggacggtaa tttctttatt
    11041 gtttaaagca atcaagcggc ttgattgtaa tccatgtgct aaaaatgcac ctgaaatagc
    11101 atttcctgca atcaatccaa taactggatg tccagcttga cggagttttg cataagcagc
    11161 agcactatta gccaaagcca catggatacc gatcaattct tcgttatagc cgtatgcttg
    11221 gcttggtaca tcgatcacaa cgatgattgg ccgtttcact tttttgtctt tatcttcttc
    11281 gtaaacgtga tccaaaattt gtcccatgcg gaaaccttca actaaaccaa cttcaccgtt
    11341 tcgtacacgg tacatcgggt ttttaggatc aggtacgatt gatgtcacga tggcttcttc
    11401 tatgaattta cttgatttac cataatacac agtactaata gaagaagttg gatttttcat
    11461 gccagttagt gccgcaaacc aatcgtaacc aacacttgct gcagctggtt catttccttc
    11521 tgctacttct ggtaagtcaa ttgattttgc ttgatggtta gcaattgctt cattgtaatc
    11581 gtcaatggcc attggttttg aaagatctaa agaatcaagt aatgataaat aaaatgctga
    11641 gctttcactg cgatgcgtgt cttttcgttc gtccattgct gcgatgacag cagctttgat
    11701 tgcttctaca ctatcagtca ctaattcatc aatgattttt gattctaccc gttgacgaga
    11761 tcctaatgta ttccaaatca agtctttgtc actagaatca aattctcgaa cgcctgcttc
    11821 ttgttcaata acttctggtc cattcaaacc gactctcgct ttttttgttg cgattaaata
    11881 actcattaat gctgaagtaa tcgacatacc gccaaatgat cctacacgtc ctggaatcaa
    11941 accgatgact ggtacatatt gttttagagc aatgattaca tttccgattt ctgaaataga
    12001 taacagtccg tagtttgctt cttgcaaacg aacaccacct gtatctagta caatgactgg
    12061 aaagatctca tttccttttt tattttcttc taacacatgt tcaagtgcag caacgatttt
    12121 tgcaccggaa acttcaccga taccgccacc ttggaatttt ccttccatag cgattactac
    12181 tgcttgtttg ccattgatcg tgccacggct gacaacgatc ccgtcatcac tttcagggac
    12241 gattccttgt gctggtaaac ttggcgcgat gatttgatcg aatggatcaa tcaattcacg
    12301 tccattgtca agtagagcgt tcactcgttc tctggcgttt aattctacaa aactattttt
    12361 catctgaaag ttcctccatt gcttgcgtca atcgtaggta cacaactcct ggtgtagcac
    12421 cgaagtcatt gatcacgata tctgctgaaa ttggataacg atcaaaaaat ctcgttaata
    12481 cattgttcca tacttcgccg aagccatcgc ttcctgtaca aatttttaca gtagattctg
    12541 ttttttctgt tggaaacatc aagatttcca aatctcctga accgaccact cctacatgga
    12601 ttttcttatt gatcggctgt ttcgttggat atttataagc taattgttcc atggatctct
    12661 cccctttttg taaaacagag catacacgtt ctgcttattc gtctttcttc ttccatctgg
    12721 gtaaatggcg acaaaaggtt tttctcgcca attttatgac caagttctga atttcattgg
    12781 tggttcatac aggccatctg accattcgat caaatcatcg atggtttgtg cagctaataa
    12841 tgaacgtttc gcttcactac gtttgatgcc tagatcttct ggtaatttca cgatgccggc
    12901 tgcacgcaag ctatctagtg attctttcgt gctagtcatt ccaagaggtg taacaccgcc
    12961 aattgctgca attgctgctt gtcgttcttc ttttgttttt gctttgtaca aataagcaat
    13021 tccttcttct gtcacgatat gtgtagtatc ttcgctataa atcatcactg gtgcattggc
    13081 taagcctgct tgtttttgaa cagctagtgc atctaattct tcgacaaaga ctggtttttt
    13141 atttgaacca tatgtttcaa ccatttgaac aactaatttt tggcctttcc ctaatggatc
    13201 atcattgtcg cgaagtgatt gccatgctgg tgtagaatga cgacgaccgc caggattatg
    13261 tcccatgtta ggcgcaccgc caaaaccaga taaacgtcct tttgttacgg tagaagagtt
    13321 tccttcgtaa tctaattgca aggttgaacc gataaacata tcgatggcat attgtcctgc
    13381 agcttgtgat aatgttcggt tactgcgcat cgtgccgtct ttaccgacaa aaaagacatc
    13441 tgggcgagca gcaatatatt tttccatacc aacttcgcca ccaaaactat ggatgctttc
    13501 aacccaacct gattcgatgg ctggaatcat cgttggatgt gggttcaata cccagttctt
    13561 cgcgattttc ccttttaacc ctagtgattc accataagtt ggcagtaaca attcgatagc
    13621 tgctgtattg aatccaatac cgtgattcaa agattgtacg ccgtgttctg cgtagattcc
    13681 tttgattgcc atcattgcca ttagaatttg caattctgtg atgttttgag gatctcgtgt
    13741 gaataaagct tctaattgat aaggtttatc tgctttcacg acaacgtcta cccagtcacc
    13801 agggatatct acacgtggta aagaatcgac aacttcgttg acttgaacga tcacgatacc
    13861 atctttgaaa gctgcagctt cgatgattgt tggtgtttct tccgtattga aacctgtgta
    13921 caagttgccg tctttatctg ctttatctgc agctactaaa acaacatttg gaatcaaatc
    13981 gatgaataag cgtccataaa gttccaaata agtatggata tcacctaatt tcattgtgcc
    14041 gtcttcaatc atttgggcga cacgcaagct ttgtgcacca gcataagaga aatcgatctt
    14101 gcttgcgatt cctttttcaa aaatattcaa atgttctggt cgtgagatac tagacatgat
    14161 caaatgcaga tcattcactt tttctgcatc tactttttca agactttctg aaagaaaact
    14221 tgcttgtttt tgattgtcgc cttctaatac aactttgtcg ccgggagcaa tcagtttttc
    14281 cattacttct acgatttttt cagtttctac atacttgcca ttgagcaagt tgctgatttt
    14341 ttccaatcgc tgcgcttttt tttcgcgttt agaagcccag ttgcggtttg gtttttccat
    14401 aattagtcat ctccattttt gatatagaaa cgtggcgtca tcaataccat gttggtctag
    14461 attgatcgta cggatccctt tatctacacc aaattttgta tgaatcagct ttgtcaaagt
    14521 attgccgggg ggaaattcaa tcgtcagatc cattcctaat tcttttgcca agtccatcat
    14581 ttggttccac tgcacaggga aagctaggtt ttccaacaaa tctttacgaa tctcttctgc
    14641 gtcctgagtg gctcttccat ccgtattttt tatatacaaa catctagcat cagatagatg
    14701 gacagactgc atatacggct tgaatttctt gaccgtatca ttcatcaaga gacagtgaga
    14761 tggcacaggg actttcagca gcttcgctgt ttgcgcttgg tgccgtttgg cttctgccaa
    14821 cgtaacggat aaaccttcga tactcccaga taacgtatgt tgcatcggac aattttcatt
    14881 tgacagataa actggatggt caggatcaaa tgtttttgct acaatttcct cagcttcgct
    14941 tctcgtcaca cctactacta cgcccattgc ataaccgctt ggataagctt ctttcattaa
    15001 gactgcgcgg tgatagacta gttccgctgc attctcaaat ctcaaagatc gacaagcaac
    15061 agctgcacaa aaagcaccaa tcgaatgtcc tgctgcaata tcaggctgaa taccgttttt
    15121 ttccatttca tctaaataaa atactgcttt tgtcagtaat gctagctgga taaaaaccgt
    15181 atcttgatag ttttcttctt tatcaactaa ccgatacccc gtcacatttt ctaaacgttc
    15241 catgtacatt tcagggatat ccgctaacat ccctaacttc tgactgcctt gtccaggaaa
    15301 taagaaacct gtactcatga gcctcgcctc ctcagcactt actttttcca ctgctatctt
    15361 agtcaatttc tgctataata aaaaatgcca aaaaaagaaa aaaccataac aaaaaggtat
    15421 ggatgggatg gataattatg aaattgcttc atctgcagta ttttaaaaaa gtattcgaaa
    15481 caaaaaatgt tacccaagca gccaaagaat tatttatttc tcaacctgcc ttgagccgtg
    15541 cgatcaaaca tttggaaaac gaattaggtg tccctttgtt ttaccatagc ggcagaaaca
    15601 ttgagacgac cgtttatgca gaagaatttt atccatatgc agtcaagaca ttagacacat
    15661 tagaagaagg aatcaaatta ttgtcttcgg tcaatgagaa gatcgttact tccgtcatat
    15721 tgtatttaga ggtagcctct gtttctattc cgaacttggt ccgaattttt atggaaaagc
    15781 atcctgatat ccaattaacg atcatgcaac atcatttacc agatgatatt accgatcctg
    15841 tactgtacat cacttcggag atgaagcctg gcatgaccaa tattcctatc atgaaagaac
    15901 ctgtttatgt cgccattcct aagaaacatc ctcttgctaa aaaagaacat ctgcaattag
    15961 aagatatcca acgcaccccc atcttgatgc tgtcaaagag caacgccttc cgtcatacac
    16021 tggatgcagc acttgaagcg aaaagtatcg aattgacgat cagttcgacg acagatgatc
    16081 ctgcgacatt acgttctatt gtcaagcaag gattagctgt tagctttttc ccaaaagttt
    16141 cttggtctta cgataaaaat gatcctttcg tcttgcggga aatcaaagat ttgcccttga
    16201 cacgaacaat ttatttatct tctagcttca cagaagatca tcctttgatc aaaatgattg
    16261 caaaaacgat gcgggaattt tatttaggaa aataaaaaaa aaagagctgg gacaaaattc
    16321 ttgtcgcagc tcttttttcg aacaaacggt gttccagaag taatttctta gaaaataagc
    16381 cgaaatttcc caaaaatttg agaagcaatt ttcgtaaatt ccttcttatt tcttgaagct
    16441 aaacatttct gtcacaacct cttctctatt gtcctagaca ttcattgcgt tgatttaaaa
    16501 cttccatcaa tcaaatctgc tagttcatct agcttttcta ttttacgtag atcaatagag
    16561 atccaatgtt ctttattcat gtgataggct ggaaaatacc cctcttttcc tcgcaaaatt
    16621 ccgccaagtt caggatcgac cttcaaatca agaatattga tgatcctatg gtcacttgca
    16681 tcatcataga ttttttcttc tggaacagac atcaggaccc caaaccattt tttgcttttt
    16741 tgatgtctga atacgcaata atcaggaaat tttttaaaaa tatattctgg agacaaattg
    16801 tatgtttcat tgatgtgatc gattagttct tttttcgtta gcatctgctt ccatctcttt
    16861 cttattgatt cgctttattc aaagatacca cgtaagaata gaaaaatgaa tacaagtagt
    16921 tgctaatcaa atacttcgat atttttcttt tttatcaact cttaaatgtt caatccgcct
    16981 gtgttggtct tgctaccatc tactattaaa atgtttatta ttgtttgccc acctattttt
    17041 tgattttagt ggtatcattt ttcaagttca tgatgaaatt cttatcatca gtaaatgctc
    17101 cagaaaaata aacaatgatt gaaaaatcag ttgaaacttg accaccgttt attttctccg
    17161 tattcttaga atagaaggga gtctgattga tgtattcact aatgaaaaaa atcattaccg
    17221 aaaaagatat tcgacgtcat gtttctttag tagaacaatt gctgaatcat acaaagataa
    17281 ccgtaaatga attagctgaa atcataggga caaccgaacg aacgattttt tctgaccttc
    17341 aatctattcg ctctcagctg cccgaaggct gggacatcat cagcgatcaa gcaggaatca
    17401 gtttgcaaaa ccagcaaaat ttattaacaa atgacctttg ggagattttt ttcaaacaat
    17461 cagtcagtgt cgaactgctg aaaaatctgc tttttacaaa aaaagttgct gtccctgatt
    17521 ttctagccga ccatgggctt tcttatggga cattgaaaag acatgtgact aaaatcaatc
    17581 agcgtttagc aagttacgac ttgcagatcg atttgacgaa atatacagct tgtattcttg
    17641 gaaaagagcg tgcgattcgt actttttatc atcgattgct gattcctttc actcataaca
    17701 actatttttt cgaggattat tcgatccatg aatcccatta ttttcaattt ttaagaaacc
    17761 tcagtcaaac tgaactagcg gttgaaacag aagagatttt cggaacatgt tggttcttta
    17821 tcaatacgat ccgtataaaa gcgaattgcc gattagattc ttctatccat accaacagca
    17881 cactctcctc cctgtatgac tctgcattga aaaaattgta tttaacggaa ggtatttatc
    17941 ttaaagacac tgagctttct tttgcttctt tctgctttct ggaaagttgg aattacaaca
    18001 ataactatgg acaagaaacc gctcgctgcc tgcatcattc tccattttta gaggtcctcg
    18061 aaacgtttgt agaggaacta gcttcagagc tgtctcttga tcaactaaaa aaaacatctc
    18121 tgactgataa cttggctctc ctcatattaa aatatcatga atcgccgata ttgattgaag
    18181 cattggaccg tcaataccat cgttttttag aaacatatga tcagcaatat gcacacttgt
    18241 atcaacagaa agagaattta ctgaacgatc taaaaaaaga aatagaaatc aatgaaccaa
    18301 attactttct ccaactcttg tctctactga tccaacaaac gattttttct atcaaaccga
    18361 atttgttcaa tgtctatttt attttccaag gagaaccttc atggaaggcg ttcttgcagc
    18421 aggaattaaa agattactta ggaaaacgtg tctgcttctt acctatcgaa ctaaccgatc
    18481 tttccactat ttcttttgaa aaaacagata tcttgatttc caatttccct ctagattctc
    18541 tagatatccc tattgtatac atttcttcta ttccaacgaa aaatgagctc aatcgtttga
    18601 ctgaactcac tttcaaaact tttttatagt aacggctagc gactgtattc tctagctgtt
    18661 ttttcttttt tgcgaaacag tttgttcatg atcccttttt ctatttctac aactaagaat
    18721 agaagaatca catttgctcc aatcaataac tgttgtgtca tcgttaaact tactgtaccg
    18781 atcagctgct ggccgactgg tagaaaaata accatccctt gcaataaagc aaggatacct
    18841 aatgaaagaa aaagcgcttt gttttgcaaa agaccttggt tcaatgacgg cttggacaac
    18901 tctctgcaat tgatcatgta aacagcttga gctaagacga tattttgcag aagaatggtt
    18961 tgctgtaagc tttgcccttc aaactgcata gccaataaat aggcaggaac catgatcagt
    19021 agggagacat agatgatccg aaacaaatga taaccagata ggattccttg ctttggatct
    19081 cttggtgggc gcttcatgat ttctggatct ggcgactcga accctagtgc ataagataaa
    19141 gtgattgtcg ttaccatatt gacccataaa atctgtactg gggacaaagg tagcgggcga
    19201 ttcagcataa gtgcccaaat caagatcaat ccttgagcta atgcagttgg caagaagaaa
    19261 ttgatggttt tttgcaaatt atccaaaatc cgccggccct cttttacggc ttttgcaatc
    19321 gtatggaaat tatcatctcc taaaaccata tcagctgcct gtttgcttac ttcactacct
    19381 ttgattccca tagcaattcc tacatcggat cttttcaaag caggtgcatc atttacgccg
    19441 tctccggtca ttccgacgat ttctccattt ttctgcaaag ctttaacaat tcgcagttta
    19501 tgttctggtg tcgttcgagc aaagacatcg actattggca cttgctgaat caactcttca
    19561 tcagacataa gatccaaatc gatcccttct aaaactttgg cagtatgttt taagccaatt
    19621 tgttccgcaa tcgcttttgc tgtgtctttg tgatcccctg taatcatttt cactgagatc
    19681 cctgcttgtt ggcattcctt tacagctttg actgcacttt cttttggcgg atcgatcaat
    19741 ccggcgatcc ctgcaaaagt taattgggtt aaagtctcat gcgtgagttc ctgtttcgaa
    19801 tctacttttt tataagcaaa tcctaaaaca cgctgacctt ttctcgcaaa ttctgcagct
    19861 gtctgactcc atgcttcttg ttctgcagaa gaaagagttg atttagcaaa taatacttct
    19921 ggtgcacctt tgacgtaaat caccgctcct ttttcctccg catgcatcgt tgccatatat
    19981 ttgtattcag aactgaacgg aatctttgct tctatttttt tcaaaggtag attggctgtt
    20041 tctgtgtgtc gaatcagcgc aacttctgtc ggattgccac ttaaattttc tgttttctgt
    20101 tcttcttttg tttttaattc ctgacagttt ttcataatgg acaacacgca ggcttcttct
    20161 tttgcagcta catccacaac tgtcatttca ttttttgtca atgttcctgt cttatctgaa
    20221 cagatcactg tcatagaacc taatgtttct accgaaggaa gtcctttgat gatcgccttt
    20281 tcttttgcca tttcatgtac tccaacggat agaatcatgg tcaaaacagc tggcaatcct
    20341 tcgggtacca ttgacaccac caaagcaatc gtagccgaaa gaaggatatg ccaatccata
    20401 ccatatcgaa atgtcgtaaa aataactaag aataggctca aacaaagaat gccttgaaaa
    20461 atttgtttgt ttaatcggtt gattttttta atcagaggcg tcgtttgttg ttcgaccgat
    20521 tgcaatgctt gattgatttt accgatttct gtcgtgtttc ctgtttctac aacgacaccg
    20581 actgcagaac cagattgtac aagcgttcct gaaaaagcca tatttttctg gtcgccgatc
    20641 gattcttctt ctgtcaaaac ggcatgactt ttttcagatg cttctgattc tcccgtcaaa
    20701 atcgattctt ccactaataa atcatgtgct tcgattaatc ggatatctgc tggaacgaca
    20761 tctccggcac gtaagacaat gacatctcct tttaccagtt ctttcgcagg gattttttct
    20821 tgcatcccgt ttgatagtac tatcgcttct tgtcccatca gctgcttcaa accattcaat
    20881 gattcttccg cttttctttc ttgccagtaa ctaacgagtc cattgacgat cactacgaat
    20941 aaaataatac ttccttcaat atattcacta gaagcaaatt tcaaaaaaga ggcaatgatc
    21001 aacacgatca tcagcagatc agtaaaatgc tttgctattt ttttccatcg ttttgtctgc
    21061 tgccttgctt ttatttcatt atatccttga tcagtcaggc gtttctgtcg ttcttcttgc
    21121 gacaagcctt ctgttgatgt ttgatagttt ttcaatacat cttctaccgt caatctgtac
    21181 cagttcatcc tatcccctcc tgtttaattg atactgttat tatacgcagg gaagtttttt
    21241 caggcgtatc gattatttca ggggggtctg aaataattcg ttttgcaaaa tgtatctggg
    21301 cctaactgat atttctattg cttattcttt tatatacaaa atttaagaat acaaaccaga
    21361 taaccaataa aaaaggaata aagcgtgtcg tgctttcgaa taacaagctg aaagcaattg
    21421 aagtaaaaaa caccaacaaa gcaagatctt tatatctttg tgtagtgttc cgtttctttt
    21481 ctttaataaa ttgaacatgt gcacacaaga tcgaagacca cacaaataga aaacaactcg
    21541 ttgtcatgcc tgccaaaagc tgaaaaaaca atcgactatc agctacaaaa aaatgtagac
    21601 aaagaccggc aaaaaataac attcctgtaa ttaaaatacc gtgtaaaggt acgcctttct
    21661 tagaaagcgc acaaaaccaa tggggcaaac ttttttcttt tgctgtttga tataaaatcc
    21721 tgctagtact gtagactgca ctatttgctg aggacaaaga agcaaccaaa atcattagat
    21781 tgacaattgc tgctccgcct tggcaatgga tcacttgcag catagtcact aaaggacttt
    21841 cttttaaatc taaacaatgc catggaacaa caaataaaat cgacaaaatc gttcctatat
    21901 agaaaaagag aatacgccaa ggcagacagt tgattgcttt tttcaaattc ttttgcggat
    21961 cacttgtctc gccaacggtt aagccgatca tttcaattcc agcaaaactg aataaaacca
    22021 taggaaatga gactaccatt ccagcaaagc caaacggtag taattcatcg atctgtccaa
    22081 aattttcttt gattccgata gactgggtat gaaaatctga gacactcaat cctataccca
    22141 ataagataaa aaggaaaatc cccaagatct tgatgattga gaaaaaagat tccaattcac
    22201 caaaccactt gactgcaaca aggttgactg ccattaagat cattagtgcg ctacctccgc
    22261 ttatcaaagc tggtacatgt acaaaccaga aatggatata caatcctatt gctgttaatt
    22321 ccgcaagtcc agctccaagc cagcaaagcc aataagtcca attgacaaaa aatgcccact
    22381 ttttgcctaa ataatagtca acgaaatcac tgatagagtt aaatgtacta tttgctaaaa
    22441 ataaatcacc catggcccgc atcaaaccat aaatagccaa accgcatatc aaataaacga
    22501 gaataacagc tggccctgct gatgcaattg cctttgctga acctaaaaat aatcctgttc
    22561 caattgcgcc acctaatgcg attaactgga tatttcgatt agttagagtc cgtttcattg
    22621 ccggtttttc catcaagttc acctttttct ttttcaagca gcatggcgac ttcctgttcc
    22681 caagaaggtt tctttatttt ttcaatacag agtggtacta caaacaaaat gacactgatg
    22741 gctgtgactg caattagatt gatccatgtt ccagctttca tcgtcagtcc gacagaaaat
    22801 ccgattgccg caaataaaac aataacgaca gtccaaactc caagattgct ctttttctct
    22861 ttttttccaa ttctaaatgg tcgattcaac tttgcatgtg tataacgcaa tctcaaaact
    22921 gcgatcccca tgatgacata gacgatacag tagataacag tagttgaatt tacgagcatc
    22981 aaaaatgctt gattgacgcc aggaatcagc aaataaaaaa gtgcaaagat tgagataatg
    23041 acagactgag tcaaaataac ggatttggat acgtcaaatt gattcgtctt ccaaaaattg
    23101 aattttggtg gatacgcacc ttttctagca ctttctgtca ctgtctttgc tggtcctgag
    23161 gcccaagcag ataattggac tgctacacca atgaagacaa gtaaactaaa cagattcaca
    23221 ataagatgtg gcaaaccaag aatttggcaa taaatcgaaa tagattgcgc gatattattc
    23281 aattccattt gtccttttgg tacgacattt gctacaacta aagcattcaa aatattgacg
    23341 aaaaatataa aaattaacgc ggcaaatact ccgcgaatat acgttttaac tggattttcc
    23401 aatcgagtga tatacacaga ggacatttcg ataccagtaa aaataaacat gatcggcgca
    23461 aaataaacaa atgaggatgc agtcgtagta tctggtatta atttgtccca tgaaaaactg
    23521 cctaatgttc cggttggtac gatcccaact ttgatccacg cagcaaaccc taataagagc
    23581 atcatcgtca aaggcacgta caatcctaac caaacaccga cttttccgcc gatttttgcc
    23641 atatcaaatt tcaaattcaa aagagtgatg atccaataaa cgactaaaat gacaactaat
    23701 gtaaatttgc tgttcaaacc aagcggcaca ttatcgataa tattaccaaa caacggagcg
    23761 agtgctgaag cgaccatcac catcccagga aacatttgga cccataacag ccatgagaca
    23821 acgaaccccc atttttttcc tagagcattc gatacccaaa gttctggacc acctttttgg
    23881 ggaaacattc cagcaaattc accagagatc aatgagattg gaaaagcgta caataatact
    23941 gcaaccagca tgtaaaaaat catcgtccag ctcgttgctg ctacatctgg aatatttcgt
    24001 acacttgcta caagggcgca ggtcattccg ataaaggtca tggtcgtgat ttgctttgat
    24061 gaaccagtat ctttcatctt tattccttct ttcaatagcg acatatgttt ttgttttctt
    24121 ttatttttgt tcaagaacag aaatgacaag gtttcatcga ttcagtacaa aacccagtat
    24181 caccgaaaca tccattgaac gatcttagtg aactgggttt gatacatgaa acgaacgatc
    24241 caacttatag aacgttttat aaaaaagaat atcttggcga ggaatatatg aaaaacaggg
    24301 ggaaatacaa ttgttttgat gaagcaaata attgtctgtt ctccatatgt ctcttgttat
    24361 tctttcacac aaaaatcagt tctattggat gatttcgttt agtttctttt ccattgcttt
    24421 ggcgattttt tttgtataaa aattgaaaat tcgatcatca ttcaaataag gagtcatgat
    24481 agccgcacgt agcagagtga ttttctgttc ttttttccat tcttctttcg ttagacccat
    24541 tctttcaaca aaacgaatcg gagaatcacc gtaatcttct tgtgtaaatg tagtatgaga
    24601 agtaataaac cgttcaccat aaacatcgcc atccatataa gaagaccgat cgaacatttt
    24661 ttcatttaat tcattgatag cgttcaaatc tgtacagcct tgttctttga atacccaatt
    24721 gaccatatta aaatcaggat gattcaatgg atagacttcg attgtttttc ctttaacagt
    24781 aaaagtcaga tgatcgagaa attctctgaa tctttgagct gcctcgattg aagccccaat
    24841 caattgacca tatccggtca cattcaatgg caatacacga tgtgctgtcc agactgcggc
    24901 ggcggttgct ccagcttttg atccttccaa aatataagct cctaacatat ctggagcctt
    24961 tactgatttt tcaaacacat atggcgcgaa ataagaaatg atatttctca tatttttatg
    25021 tttgattacg attcctccag cagcataagg gacatagccc atcttatgag gatctatcgt
    25081 gacagaatcc gcttctgaaa tagctctgaa cccttcatat acctctttgt caatcgttac
    25141 gcagtggtgg aaaacatgat gttcttcaaa gaattcagct aaggaagcat acggcacaaa
    25201 ttctcctgct tcgtttagaa acagtgaacg cgcatagccg ccatatgcgg catcaacatg
    25261 taaatagaaa tagatccctt catcttttaa cctttctctt aattggacga ttttatctac
    25321 actgtctact tgaccttctt ctgtcgttcc tacgacagca accacaccta gaattgggat
    25381 tttttgtcca gctaattcac gaattgtttt ttccaaagca ttaatatcca ttcgataatc
    25441 ctcttgaact gggatagcaa ccatctgatc taagccgact ccgcaaatat ctaacgcctt
    25501 catccaagaa tagtgttttg tttgagggac cagccatttc cctaatcttt ggatattttt
    25561 tccgctacgg gaagaagctg ctttgacctc atccatttct tcgtccgtaa agctttcggt
    25621 catttccaaa atttcttcaa cagataaatt caaaagttcc cactcagaca tttttttcac
    25681 tttttcaggc agcacttcct tgacagctaa aggaatcgat ttgatgcaac gggcgtacca
    25741 taatccttca agattagcaa tagaaccatc tgctgcaatg tgtccccatc cgtctgtcat
    25801 attgaataat gaagcaaaat cctgtcctac ttctgcttcc atttgcgaag ttgccataga
    25861 agattccaat gcaacattat ttgggttcca cagcatcgca tagttgtaag ctaataaagc
    25921 aggcatcaat gtttcagcat tcatttgtcc ccagtaacgg cctgctgaat gccatgggat
    25981 cgatcctgcg cgtaatcgct gactgacttc atccaagacc tctatcatat ggtcgcgggt
    26041 ggctagataa tctggagtga gcttatctcc ttcgctaatt gcaggcatat ctgaaggaat
    26101 ataattttct ctccatccaa gatgttcatc taccatcttg ttcaacaaca ttttataaac
    26161 tggtccattt tcagctttgt cgcctataaa gacggccttg atatccatat ctttcatttt
    26221 tcttccctcc atattttgtt ggtcaatgtc ttgcagatac atgtgtttat ctgtaacacc
    26281 tcttcactat agcgccaaaa atgagaaatt
//
LOCUS       DACDKM010000024        25500 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20834_2016
            SAMN12640370-rid9891663.denovo.024, whole genome shotgun sequence.
ACCESSION   DACDKM010000024 DACDKM010000000
VERSION     DACDKM010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640370
            Sequence Read Archive: SRR10040891
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 25500)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25500)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742835.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742835.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:04:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,012
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 29 of 75
            Pseudo Genes (incomplete)         :: 33 of 75
            Pseudo Genes (internal stop)      :: 19 of 75
            Pseudo Genes (multiple problems)  :: 5 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25500
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640370-rid9891663.denovo.024"
                     /isolate="E20834_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            132..3659
                     /locus_tag="IV334_001039"
     CDS             132..3659
                     /locus_tag="IV334_001039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HAP8943128.1"
                     /translation="MSLQFILGNGKKDHRQVLIDEASAWLDQDASNQVFFLVPNYSKF
                     EQEQEILAEMRRRSGKKTFSTTNVQVFSFYRLAWYLLQQTTLLTGNELSESGSAMILR
                     QILEKNEEELTIFRGEINKRGFIRQLQELYSELRTGNIQPEDLMLLFGDSPNAEDQQL
                     KMKDLKLIFSAYDSELTQRALTNEDALSILANYMQTQDFNNVKFIVSGFSRINGQEYQ
                     LLQAMMEKGQLVVDLLLDRPYTADLPEVLTLFHETGKLYFRLFQSARERKVPILVDRF
                     APEQTRPTELQLLQTMWEETSSQKKAAVHKIGGQEHLHLWEAENPTEEVRQLAVEIRR
                     LVSEENYRYRDIQVLTKNMSLYGNILRPIFQEMAIPFYIDEEQMMENHPLIEWINSLF
                     ALDRYSYRLSDIMRFFKTELFLSTEAETELDEWKQERDEFRKKLDITENVALAYNYQG
                     TYWTREKDWQFVAYDFEAEQIEDVQYLEEQSNTIRRLFQKAVPVFLKKIKKSKTGQEA
                     ANVFYQFLIQSGVQQQLLFWRNQEVDRGNLEAARNHEQTWGALIDLLDEYVVIYGESA
                     FDWSLFQEIITSGLENLSYGKIPTAIDQVRINRLELVRPNQAAVTFALGLNDQVFPDR
                     KETKGLLSSEEREVLNQALPDGQFLFDPSKESISFEPFQAYLVFLSGTDRLYLSYAQS
                     YDTDGSLKMSPYLRRITEYLSVPVERKEHLTIESDPNCYVGTYRSGINTVNRIYRLAL
                     DEKKPVPKHWQKLKELVLNSSWRNFALQIFESQEHLNVPVSMPEKMAEELYGKEIYTS
                     ISRMENFYNCEYKYFMQFGLKLKERTVYGLTPAATGDFYHESLDRFFKLLFSNQLSLV
                     NMSDAQRRDFTEKVLQEVFGELRFEILDSSARMNYIRYQLGQTIQKVTWALQKQSKNT
                     GMHPLQTEILFGQIAGAKGITGLELPLSNGGKLHVRGKIDRIDVASEQEDTWLSVVDY
                     KSSGRSFDVTEAYYGMAMQLLTYLDVALMDAVRLTGKAAVKPAGSYYLHVHNPVLTET
                     DDVEKNTLKKFSYDGVFVDDPALLEVLDHSLEAKEQSLIYPVKKNAKDEYQKGSFSKD
                     KFFTEEELQLFMAHNRENMRLAGDAITSGEIKLNPAYKDKQRIACEFCPFRSICTFDV
                     MLKENNYHRLEKIDKEEALRRILKRSEEE"
     gene            3656..7378
                     /gene="addA"
                     /locus_tag="IV334_001040"
     CDS             3656..7378
                     /gene="addA"
                     /locus_tag="IV334_001040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-exonuclease AddAB subunit AddA"
                     /protein_id="HAP8943129.1"
                     /translation="MNQIPLKPQNERFTDDQWQAIFDQGDNLLVSASAGSGKTTVLVR
                     RVIEKLKMGFDIDELLIVTFTEAAAREMKERIQEALQESVNSESDPVRRQHFTKQLVL
                     LPTANISTLHAFCLTVIRRYYYLIDIDPVFRMLTDETETILMKEDVWDELREALYAEN
                     DERFFQLTMNFSNDRSDDGLTNLVFSLYEFARANPDPQKWLEQLSDNYRLPEGLAKSR
                     LYQKQIRPLVLADIYQCVQLYEQMTQLAQGEGLEKMHEQVAGEQQQIKNIYEAFSQDR
                     LEEAYAGLEQLTFSTFKSSRKAELKEISNEVKGMRDKAKKLIQQISKSYFPVSPSQME
                     ELTDKALPLVEEMTKVTQSFMDGFSMRKREKGVLDFNDLEHLALQILTEKTKDVWLPS
                     EASKHYRKKFKEVMVDEYQDVNQLQEAILYWLREPDDTKGNMFMVGDVKQSIYSFRLA
                     DPSLFIGKYENFSKKEGGRRIVLAENFRSRKEVLSFTNLIFEQLMDPAVGQINYDEAA
                     KLIQGFSDFPENEQFEPEIMIYEKEQEESEIEIPTDDILEDKTEGELFMTGLKIRQLI
                     DSSFMIYDKKSKKSRPIEYKDIVLLTPTKKNNLTILEIFKTLDIPLEMNDAQNYFQAT
                     EIRTMISLLQLIDNPYQDIPLAAVLRSPIVGLIEPELASIRLANRAHTYYDAVLAYQA
                     SNEDELAAKLEHFGKQLEHWRELARRSSITDLLWDIYYETGYLEYVVGLPAGAQRQAN
                     LYALVDRAKAYEQSSFRGLYQFVRFIEKMQEKDKDLAEPVISIEDNAVRVMTIHASKG
                     LEFPVVFLLDMTKEFNLQDLRNRYAFEEKLGAGIRYMDPETRVLYDTLPFQAIKLAKQ
                     NKLLSEEMRKLYVGLTRAEQKLFIVGSYKNKEQMIQTWSEAADHEELVFDPALRLKGR
                     SSLMNWIGYGLIRHPEMQKYLEEEISTSLLQHSNAQFSISWMNQQSIIEQRQLLAEKE
                     LVNLDQQMKEDETLLADSLQKRLAYEYPYQASSQTTSYQSVSEIKRLFEDPDDTQESR
                     LTLESSQNKAASRQFRYTQEQLAEPKFLQKDRQVSAAAVGTATHALLQLLPLEMPTTE
                     SIHQKLQELVRKRLVDEKVAKKVDVSSIIWFFQTELGQQLIANKENVKREQPFSMLLP
                     ADEVFQDYPNQEDELLIHGIVDGYLEEKDHLNIYDFKTDFILHPDDPAEIDAIVQKYQ
                     GQLRLYQQAMSEALNKPVENVFLILLRVKQIININK"
     gene            7447..7788
                     /locus_tag="IV334_001041"
     CDS             7447..7788
                     /locus_tag="IV334_001041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP8943130.1"
                     /translation="MELTQTEFVLCPKFEKTFSILGKKWNGLIIDVLLENGPQRFVEL
                     AAKIPMVSDRVLVERLKELEKEGIVTRNFACSEKSRSEYLLTEKGADLQKAMRQIQVW
                     SEKWITEEECG"
     gene            8097..9140
                     /gene="pheS"
                     /locus_tag="IV334_001042"
     CDS             8097..9140
                     /gene="pheS"
                     /locus_tag="IV334_001042"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HAP8943131.1"
                     /translation="MSLQAQLESLKEATLVKITAAQDLNELNQIRVETLGKKGPITEV
                     LRGMKDLSPEERPKVGSFANEIRDLLTEEIERKKQVLEEEALNAALAKETIDVTLPGR
                     KVKHGTRHIVTQIMEEIEDIFVGMGYQIVEGYEVESDHYNFERMNLPKDHPARDMQDT
                     FYISDEILIRTHTSPVQARTMEKHDFSKGALRMISPGKVFRRDTDDATHSHQFHQIEG
                     LVVDKNITMGDLKGTLEVVMKKMFGEDREIRLRPSYFPFTEPSVEVDVSCFKCGGAGC
                     NVCKYTGWIEILGAGMVHPNVLKMSGIDPEEYSGFAFGLGPDRVAMLRYGVNDIRNFY
                     QNDLRFLSQFKGE"
     gene            9145..11565
                     /locus_tag="IV334_001043"
     CDS             9145..11565
                     /locus_tag="IV334_001043"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HAP8943132.1"
                     /translation="MLVSYKWLNRYVDLSNVTPKELADKMSVTGIEVEGVTVPEEGLK
                     KIVVGEVKECVPHPDSDHLSICQVDIGEDELSQIVCGAPNVKVGIKVIVALPNSRIAG
                     NVKIKKGKMRGQVSNGMICSLQEIGYSDSVIPKEYAEGIYYMPQEAVNGDPVFPYLDM
                     DDSIIELSITPNRADALSMRGVAYEVGAIYRQTPTFDDEKLVESSRPASDKIQVEVED
                     KEAVPAYQIRIIEGVRIQPSPQWLQNLLMNEGIRPINNVVDVTNYILLLFGQPLHAFD
                     YDKLKSKEIFVRRAKENETLVTLDGESRELTTDNLVITNGKEPVALAGVMGGLDSEIT
                     NETTTVALESALFDPISIRRTAKQFNLRSESSARFEKGINHATVGEASEVAAAMIVSL
                     AGGEVLQGAVKGSEIKAEDVSVKISLERINQYLGTSLVVKEVNEIFEALGFSYEEKEY
                     VYSVVVPPRRWDIQIEADIIEEVARIYGYDQLPSTLPSGETVAGSLSKEQETTRKIRT
                     IFEGSGLSEAISYALTTEEKSRQFVINESNITRLDWPMSEERSVLRMNLISGLLDDIS
                     YNTARKNTEIALYEIGRVFFQENDPRTHLPQEVKHAAIAISGIWEEKDWQTKAQNVDF
                     FTIKGLLENLFEQLGITEDVHYQTVTEMKEMHPGRTAAIYLGEKFIGFVGQVHPTITK
                     SYDIPETYVAEFDLSAIVEAAQKGIVFEAVTKFPAVSRDIALLVKETVTNQELTEVIR
                     SAAGRFLTDIQLFDVYQGENIEKGHKSMAYSLTFVNPEATLTDEEINKAMEKVEKALV
                     ENLEASIR"
     gene            complement(11735..12745)
                     /gene="trpS"
                     /locus_tag="IV334_001044"
     CDS             complement(11735..12745)
                     /gene="trpS"
                     /locus_tag="IV334_001044"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HAP8943133.1"
                     /translation="MKNIILTGDRPTGKLHLGHYVGSLKTRVAMQADEDNQLFVMIAD
                     MQALTDNAKNPEKVSSNVLQVALDYLAVGLDPAKSTLFIQSQIPELAELTMYYLNLVS
                     VGRVRRNPTVKTEIEQKKFGESVPTGFFIYPVSQAADITAFKANLVPVGEDQKPMLEQ
                     TQEIVQSFNHTYGEVLVEPKGVFPPKGMGRLPGIDGNGKMSKSLGNGIYISDPADVLQ
                     KKVMSMYTDPNHIHVQDPGQVEGNMVFTYLDVFGTDKEAIEEMKAHYRRGGLGDVKIK
                     RYLIDVLEAEFAPIRARREELEKDPSAVMEILRKGSEEAAKVAAQTLSEVKSAMGINY
                     FH"
     gene            12999..13736
                     /locus_tag="IV334_001045"
     CDS             12999..13736
                     /locus_tag="IV334_001045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HAP8943134.1"
                     /translation="MAMIKFDHVDKYYGKFHALKNINLEFEKGEVVVVIGPSGSGKST
                     MLRCINGLETISSGKLLINDTDLHDKKTKLTEVRKNIGMVFQHFNLYPNKTVLENIKL
                     APIKVLKQDQATAVKNAEKFLKTVNMLDKKDSYPSMLSGGQQQRVAIARGLAMNPEML
                     LFDEPTSALDPEMIGDVLDVMKKLAHDGMSMIVVTHEMGFAKEVADRVIFMADGEVLE
                     DSRNVRDFFENPNEVRAQQFISKVINH"
     gene            13750..14565
                     /locus_tag="IV334_001046"
     CDS             13750..14565
                     /locus_tag="IV334_001046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HAP8943135.1"
                     /translation="MRRLKKIRLIFLLGIAFLALAGCKGNGLANEDILERSKSTNEII
                     WGVKYDTRLFGMMDIESRTVQGFDVDIAKAITKKILGDNGKTEFVEVTSKTRIPLLKN
                     GNIDAIIATMTITDERKKQVDFSDVYFDAGQALLVKKGSQIKSVDDLNASTTVLAVKG
                     STSAANIRQHAPDAKILELENYAEAFTALQSGQGDAMTTDNAILLGIADENPEYELVG
                     GTFTNEPYGIAINKGQENFLKAVNQALEEMHADGTYDKIYQKWFPNETEGKVE"
     gene            14578..15225
                     /locus_tag="IV334_001047"
     CDS             14578..15225
                     /locus_tag="IV334_001047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HAP8943136.1"
                     /translation="MSELIQTYGPIFVDGFKITLFSSVLALVFSLIIGTLMAIFQLSK
                     NKVVSALAKAYVEFFRNIPLLIIVMFFYVVVPIYWFNLDGFQAGTIGLTIYTSAFIAE
                     TVRSGIQTVPKGQTEAGLSSGFTYSEIMRYIVLPQAFKIVIPPLGNQFINLVKNSSIL
                     AIVAGLDLMYQGDLIASETFNTFDTYIIVGLFYLVITLPLSYLMVYLEKKWAVRS"
     gene            15241..15897
                     /locus_tag="IV334_001048"
     CDS             15241..15897
                     /locus_tag="IV334_001048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HAP8943137.1"
                     /translation="MDFSGAFSWMNIRFLLEGLKVTVEVSLFSIIFSFLIGGLTGVIR
                     FANIPFFSKILGVVIDIIRNLPLLLIIFFTYFALPQIGIQMNIFWSAVAALTIFESAM
                     LSEIFRAGLNAVPKGQMEAGLSTGLTYVESMRTIVLPQAFKSMIPAIVSQLISLIKDT
                     SLAVIISLPELSHQARIIYGQNTNYVLPMFIIMTLMYFVVCYALSLLSGYLEKRRYSY
                     "
     gene            16059..16880
                     /gene="racE"
                     /locus_tag="IV334_001049"
     CDS             16059..16880
                     /gene="racE"
                     /locus_tag="IV334_001049"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HAP8943138.1"
                     /translation="MIRLTDNRPIGFIDSGVGGLTVVKEALKQLPNENILFVGDTARC
                     PYGPRPAEQVIQYTWEMTDYLVEQGIKMLVIACNTATAVALEEIKAALSIPVIGVILP
                     GTRAAVKKTQNKQVGIIGTIGTVKSQAYEKALKEKVPELTVTSLACPKFVSVVESNEY
                     HSSVAKKIVAETLAPLTTKKIDTLILGCTHYPLLRPIIQNVMGENVQLIDSGAETVGE
                     VSMLLDYFNLSNSPQNGRTLCQFYTTGSAKLFEEIAEDWLGIGHLNVEHIELGGK"
     gene            16883..18238
                     /gene="rph"
                     /locus_tag="IV334_001050"
     CDS             16883..18238
                     /gene="rph"
                     /locus_tag="IV334_001050"
                     /EC_number="2.7.7.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HAP8943139.1"
                     /translation="MRHDGRKAQELRKLELQTNVFKYPEGSVVIRFGDTTVICSATIE
                     NSVPPFLRETGTGWVTAEYSMLPRATSTRNRRESSKGKLSGRTMEIQRLIGRSLRAVV
                     DLEKLGERSIIVDCDVIQADGGTRTASITGAFVALKLAIEKLLREKELSEDPIKEHLA
                     AVSVGILPDGTCVTDLDYQEDSAALVDMNLVMTESGKFVEIQGTGEEATFDGEQLNEM
                     LFFGKNAIEDLIKEQKHALLTEFAQNDERIEETKTIIIATRNPGKAEEFRNMFKEAGY
                     HVKTLLDYPELPDVEETGSTFEENARLKAETIAQLLDQPVLADDSGLKVDALGGMPGI
                     YSARFAGEQKSDAGNNAKLLYELTDVPDEKRTAQFHCTLVFAAPKKDSLVVEAEWPGR
                     VARIPSGENGFGYDPLFIPEGKKQTAAELSSEEKNKISHRAQAMKKLSAEWKQWLEGE
                     R"
     gene            18239..18757
                     /locus_tag="IV334_001051"
     CDS             18239..18757
                     /locus_tag="IV334_001051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HAP8943140.1"
                     /translation="MRYLVVSDNHGDRDILVDLVDRYRSEVDIFFHCGDSELEASDPL
                     WDTFQVVQGNCDYGPGFEQKKVIQTGQDTVFMTHGHLSNVRFGLTQLAIEARAATADM
                     VFFGHTHQIGCEMDQKILFLNPGSISQPRGPIQIPSYALIESLDDQISVQYYNRAHQA
                     VEELAFSFSRER"
     gene            18864..19364
                     /locus_tag="IV334_001052"
     CDS             18864..19364
                     /locus_tag="IV334_001052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HAP8943141.1"
                     /translation="MIGPIVREMLLENQETFLIPAENVANVMYHHPLSHGLLVLSKVG
                     YSKIPVLGKDDHFVGLVSLSDVVNKMIDLQTISMDPLEGLTVADVTETDVPTIDENWE
                     LEEVLHLLVDSPFLPVVTEDRIFKGIITRKELLKAVNFMVHELERRNILSPKIEELKE
                     QIDIVS"
     gene            complement(19644..20564)
                     /locus_tag="IV334_001053"
     CDS             complement(19644..20564)
                     /locus_tag="IV334_001053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HAP8943142.1"
                     /translation="MFSLLATAGTTDSSGETLTDQAVSKVNAFQRFWDGIDWDAILAA
                     FIQKSLYLIFLIVLFWILHRVGKYLIDKSFNQYSKKAILNESRLKTLHSLLNTIFHYT
                     LGFFFIYAILSAIGVPIGSLLAGAGIAGVAIGLGAQGFMSDVITGFFIIMEQQMDVGD
                     YIKLANLTIEGTVVSVGIRTLQLKATDGTVHFIPNRNITTISNLSRANMQVLLDIRIV
                     PEEGYDKIYEIIDRVNRTLAEKYQEELQTEPSIFGLVDLGNSNFAIRTVCYALNGKQY
                     ALKEEFLSSYVKELTASGFTIPNSPIAVGK"
     gene            20729..21742
                     /locus_tag="IV334_001054"
     CDS             20729..21742
                     /locus_tag="IV334_001054"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid aminotransferase"
                     /protein_id="HAP8943143.1"
                     /translation="MQIDWNNLGFSYIKTPWRFIAKWKDGQWEEGELTEDNYLTIHEG
                     SPALHYGQQCFEGLKAYRRKDGKVNLFRPEQNSRRMNQSARRLLMPMVPEKMFVDAVK
                     QVVKANEDFVPPYGTGATLYLRPLLIGVGENIGVHPAPEYLFVVFCTPVGAYFKGGLK
                     PTNFIVSDYDRAAPKGTGAAKVGGNYAASLLPGTEAKELDYSDCVYLDPATHTKIEEV
                     GAANFFGITKDGKFITPQSPSILPSITKYSLLQLAEERLGLQAIEGDIFIDDLDQFAE
                     AGACGTAAVISPIGGIYHNNNLHVFYSETEVGPVTKRLYDELTGIQFGDIEAPEGWIE
                     VVE"
     gene            complement(21852..22607)
                     /gene="fabI"
                     /locus_tag="IV334_001055"
     CDS             complement(21852..22607)
                     /gene="fabI"
                     /locus_tag="IV334_001055"
                     /EC_number="1.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HAP8943144.1"
                     /translation="MSFLEGKKIVIMGVANKKSIAWGCAEAMLKQGAEIIYTYQNERM
                     KKSLVKLVGEEAFTVECDVSSDESIEKAMNAIGEYAGEIHGLVHAVAYANKEELSGNV
                     SDISRDGYQLAQDISSYSLIAVSKYAQPFLAPNSGIVSMTYLGSERAIPNYNMMGIAK
                     ASLEAAIRYLAAEFSPKGIRVNGISAGAIKTLAVTGVKDYQKLIQLSESRTPDQQGVT
                     IEEVGNACAFLISPLSSGIIGDIIYVDKGVHLS"
     gene            22827..23267
                     /gene="fabZ"
                     /locus_tag="IV334_001056"
     CDS             22827..23267
                     /gene="fabZ"
                     /locus_tag="IV334_001056"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HAP8943145.1"
                     /translation="MSYVLSATEVMELIPNRYPICYIDYVDTLEPGKKIVATKNVTIN
                     EDFFQGHFPDNPVMPGALILETLAQAGSILILKSEEFLGKRAYIGGINKAKFRQKVVP
                     GDVLKCHFEITKMKGSVGTALSAAYVDDKKVCECEFTFIVNQNA"
     gene            complement(23382..24143)
                     /locus_tag="IV334_001057"
     CDS             complement(23382..24143)
                     /locus_tag="IV334_001057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HAP8943146.1"
                     /translation="MKKNKVILSISLAAALLFAAGCSANNTTSSSSSTESSKSTSTAE
                     STTASSVDLNALELPQLTKDVAENEDLVEMVTNKGTIKIKLFPEHAPKAVENFMTHAK
                     DGYYNGLTFHRVIKDFMIQGGDPKGDGTGGESIWGEGFETEINNHLYNIRGALSMARS
                     QDPNSNGSQFFIVQNTDDMHDGLLKDDYPQAIIDAYKNGGYPSLDGHYTVFGQVIEGM
                     DVVDAIANIETDSSDKPKEEVKIDKINILQEAKQK"
     gene            24332..25426
                     /locus_tag="IV334_001058"
     CDS             24332..25426
                     /locus_tag="IV334_001058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF871 domain-containing protein"
                     /protein_id="HAP8943147.1"
                     /translation="MGKLGISIYPERSTFEKDKAYLDLAHKYGYKRVFTSLLQINDDK
                     EKVLSEFKEVVDYANSLGMEVMVDINPALFEQLEISYDDLSFFHKMGAYGVRLDIGFT
                     GAEEAKMTRNPFGIKIEINMSSGTNYVDNIMSYSPNTDNLLGSHNFYPHRYSGLGYEH
                     FVFCSEKFRKYNLNTMAFVNSQSAEFGPWPTQDGLCTLEDHRDLEIATQVKHLILTGL
                     IDDISIGNAYASEEELKEMAEAFNADYPTLKVDTEEGITENERICLFDNLHSYRGDRS
                     EYILRSTMTRVYYKDKDFPPHNTRDMHHGDVLIDNEGYGQYKGETQIALKDMKNDGRV
                     NVVGRISDDELFLLDFLKPWSSFKLIENKK"
BASE COUNT         8448 a         4424 c         5383 g         7245 t
ORIGIN      
        1 ttttgcgtat acttaaaaaa gtagaaatat cagaacctta aaaggatata atttgaagaa
       61 tatagaacaa atgttcagta ttttttgata taataaagga gcaccgtatc atgtgttgaa
      121 ggaggaaaag catgtcttta caatttattc ttggaaacgg gaaaaaagat caccgtcaag
      181 tcttgatcga cgaagcatcc gcttggctcg accaggatgc aagtaaccaa gtatttttcc
      241 ttgttcccaa ctacagcaaa ttcgaacagg aacaagaaat tttagcggag atgcgaagaa
      301 gaagcggaaa aaaaactttc agcacaacaa acgttcaagt atttagtttt tatcgtctgg
      361 cttggtattt gctgcaacag acaactcttc ttacaggaaa tgaattatca gaatcaggat
      421 ctgcaatgat cttgcgacag attttagaaa aaaatgagga agaattaaca atctttcgcg
      481 gagaaatcaa taaaagaggg tttattcgtc aattacaaga actctacagt gaattgcgta
      541 caggaaatat tcaaccagaa gacctgatgc ttttatttgg tgattcacct aatgcagaag
      601 accagcaact taaaatgaaa gacttgaagc tgattttttc agcttatgac agcgaattga
      661 ctcaacgtgc actaacaaat gaagatgcac tatctatact agcaaattat atgcagacac
      721 aagatttcaa caacgtcaaa tttattgtca gtggtttttc tcgaatcaat ggtcaagagt
      781 atcaattact gcaggcaatg atggaaaagg ggcaattggt tgtcgattta ttgctggatc
      841 gtccttatac agcggatctt ccagaagtac ttacgctctt tcatgagact ggaaagcttt
      901 atttccgtct ttttcaaagt gcaagagaac ggaaagtccc tatattagtg gaccgatttg
      961 caccagagca gactcgtccg acagagcttc aactgctgca aacaatgtgg gaagagacat
     1021 ctagccaaaa gaaagcggca gtacacaaaa tagggggaca agagcatctc catttgtggg
     1081 aagctgaaaa tccaactgaa gaagtccgac aattagctgt tgaaatcaga cgactagtca
     1141 gtgaagaaaa ctaccgttat cgtgatatcc aagtgctgac aaaaaatatg tctttatatg
     1201 gaaatatcct tcggccgatc tttcaagaaa tggcaattcc tttttatatc gatgaagaac
     1261 aaatgatgga aaatcatccg ctgatcgaat ggatcaacag tctgttcgct cttgaccgct
     1321 attcttatcg gctaagtgat attatgcgtt ttttcaaaac tgaattattt ctgtcgacgg
     1381 aagcagaaac cgaacttgat gaatggaaac aagagcgaga tgaattcagg aaaaaactgg
     1441 acatcactga aaatgtagca cttgcttaca attatcaagg aacttattgg actcgtgaaa
     1501 aagactggca gtttgtggcg tatgattttg aagcagaaca gattgaagac gtacagtatt
     1561 tggaagaaca gtcaaatacg attagacgat tatttcagaa agctgtacca gtatttttga
     1621 aaaaaataaa gaaaagcaaa acaggacaag aggccgcaaa tgttttttat caatttttga
     1681 tccaaagtgg cgtacagcaa caattattgt tttggcgtaa tcaggaagta gacagaggaa
     1741 acttagaagc ggcaagaaac catgagcaga catggggagc gctgattgat ctgttggatg
     1801 aatatgtagt aatttatggt gaatcggctt ttgactggtc actgttccaa gaaatcatta
     1861 caagtggtct agaaaatcta agttatggta aaatccctac cgcaatcgac caagtgcgaa
     1921 tcaatcgttt ggaattagtt cgaccaaatc aagctgccgt cacttttgca ttagggttga
     1981 atgatcaagt gtttccagac cgaaaagaga caaaaggatt gttgtccagt gaagaacgag
     2041 aagtattaaa tcaggccttg ccagatggac agtttttatt cgacccatca aaggaaagta
     2101 tctcttttga gcctttccaa gcctatcttg tttttctttc cggaacagat cgattatatc
     2161 ttagttatgc acaaagctat gacacagatg gttcgctgaa gatgtctcct tatttgcgta
     2221 gaatcacaga atatttatct gttcccgtcg agagaaaaga gcatttgacg atcgaaagcg
     2281 accctaattg ttatgtaggg acttacagaa gcggaatcaa tacagtcaat aggatttatc
     2341 gattagcttt agatgaaaaa aaacctgtac caaagcactg gcaaaagctc aaagaattag
     2401 tactgaattc gtcatggcga aattttgctt tacagatttt tgaaagtcag gaacatttaa
     2461 atgttcctgt ttcgatgcca gaaaaaatgg cggaagagct ttatggaaaa gagatttata
     2521 cttcaatttc acgtatggag aatttttata attgtgaata caaatacttc atgcaatttg
     2581 gtttgaaatt gaaagaacga actgtttatg gtctaactcc agcagcaact ggtgattttt
     2641 atcatgaatc attggatcgt ttcttcaagc tactgttttc aaaccaatta tccttagtga
     2701 atatgtccga tgcgcagcgt agagacttta ctgaaaaagt gctgcaggaa gtattcggag
     2761 aacttcggtt tgagatattg gatagttctg caagaatgaa ctatatccgc taccagttag
     2821 gacagacgat tcaaaaagtg acatgggctt tgcaaaaaca gagcaaaaat acaggcatgc
     2881 atcctcttca gacagagatt ctatttggtc agattgctgg agcaaaaggt atcacaggtc
     2941 tggaattacc gttgagtaat ggtggaaaac tccatgttcg agggaaaatc gatcgaatcg
     3001 atgtagcttc tgaacaagaa gatacttggc taagcgtcgt tgattacaaa tctagcggac
     3061 gttcattcga tgtcacagaa gcttattatg gcatggctat gcaattattg acgtatctgg
     3121 acgtagcatt gatggatgcg gtacgtttga ctggaaaggc ggctgttaaa ccagcaggat
     3181 catattattt acatgttcat aatcctgtat tgacggagac agatgatgta gagaagaata
     3241 cattgaaaaa atttagttat gatggcgttt ttgtcgatga tcccgctctt ttagaagtac
     3301 tagatcattc actggaagca aaagaacaat cactgatcta tccagtgaaa aaaaatgcga
     3361 aagatgaata ccaaaaaggt agtttcagta aagacaaatt tttcacggaa gaagaactgc
     3421 aactattcat ggcgcataat cgggaaaata tgcggttagc tggagatgcg attacttccg
     3481 gagaaatcaa actaaatcca gcttataaag ataaacagcg gattgcctgc gaattttgtc
     3541 cattcagaag catctgtacg tttgatgtga tgctgaaaga aaataattac caccgtctgg
     3601 agaaaataga taaggaagaa gcactgcgta gaatattgaa aaggagtgaa gaagagtgaa
     3661 tcaaattcct ttaaaaccgc aaaatgaacg attcacagat gatcagtggc aagcgatttt
     3721 tgatcaagga gataatctat tagtctctgc gtctgccgga tcaggtaaaa ctacagtgct
     3781 tgttcgaaga gtgattgaaa aactaaaaat gggttttgat atcgatgaat tattgattgt
     3841 gacatttact gaagctgccg caagagaaat gaaagaaaga attcaagagg cattacaaga
     3901 atccgtcaat agcgaaagtg accctgtacg tagacagcat tttactaagc agttagtttt
     3961 attgcccaca gcgaatatct caaccttaca tgctttttgc ctgacagtta ttcgacgtta
     4021 ttattactta attgatattg atccagtctt tcgcatgtta acagatgaaa cagaaacgat
     4081 cttgatgaag gaggatgtgt gggacgagct gagagaagca ctttatgcag aaaatgatga
     4141 acgatttttc caattaacaa tgaatttttc taatgatcgt tcagatgacg gcttgacgaa
     4201 tctggtgttt tctctttatg agtttgcccg tgccaaccct gacccccaaa aatggctaga
     4261 acagttaagc gataattatc gtttgcctga ggggctagcg aaatctcgat tatatcaaaa
     4321 acaaatccgt cctttagttt tggcagatat ataccaatgt gtacagttat atgaacagat
     4381 gactcagtta gcacaaggtg aaggattaga aaaaatgcat gagcaagtag ctggagaaca
     4441 acagcagatc aagaatatct atgaagcgtt ctcgcaagat cggttagaag aagcttatgc
     4501 tggtttggaa caactaactt tttcaacttt taagagcagt cggaaagcag aattgaaaga
     4561 aatttccaat gaagtcaagg gcatgaggga taaagcaaaa aaactgattc agcagataag
     4621 taaaagttat tttcctgttt ccccaagcca aatggaagaa ttaacggaca aagcactacc
     4681 gttagtcgaa gaaatgacaa aagtcacgca aagcttcatg gacggtttca gtatgcgtaa
     4741 acgggaaaaa ggtgtgctgg atttcaatga tctggaacat ttagcgttgc agattctcac
     4801 agaaaaaacg aaagatgtat ggctgccaag tgaagcttca aaacattatc gaaaaaaatt
     4861 caaagaagta atggtagatg aatatcaaga cgtgaaccaa ttacaagaag ccattttata
     4921 ctggctaaga gaacccgatg atacaaaagg aaatatgttc atggttgggg atgtcaaaca
     4981 atccatctat tctttccgtt tagcagatcc tagtttattt atcgggaaat acgagaattt
     5041 ctcaaagaaa gaaggcggac gtcgaatcgt tttggctgag aacttccgct ctagaaaaga
     5101 agtcctttct tttacgaatc ttatttttga acaactaatg gatccagcag tgggtcaaat
     5161 caattatgac gaagcagcca aactgatcca aggattttca gactttccag agaatgaaca
     5221 gtttgagccg gaaatcatga tctatgaaaa agaacaggaa gaatcagaga ttgagattcc
     5281 gacggatgat atactggaag acaaaacaga aggcgaactt tttatgacag gactaaaaat
     5341 tcgtcaatta attgattcct cctttatgat ttatgataaa aaatcaaaaa aaagtcgtcc
     5401 gatagagtac aaagacattg ttctcttgac acctacaaag aagaacaact taacgatttt
     5461 agagattttc aaaacattgg atattccatt agagatgaat gatgctcaaa actattttca
     5521 agcgacagaa attcgcacga tgatctcgct gctacaatta atcgataatc cctatcaaga
     5581 tattccgctt gcggctgtat tgcgctcacc gatcgtgggt ctgattgaac cagaattagc
     5641 cagcattcga ttagctaacc gtgcacatac ttactatgat gctgtgcttg cttatcaagc
     5701 atcaaacgaa gatgaattag cagctaagct ggaacatttt ggcaagcaac tagagcattg
     5761 gcgagaactt gctcgccgtt cttcgatcac tgacttgtta tgggatatct attatgagac
     5821 gggatatcta gagtatgtgg tcggtctgcc agcaggggca cagagacaag ccaatcttta
     5881 tgcattggtt gatcgtgcca aagcttacga gcaaagttct ttcagaggat tataccagtt
     5941 cgtgcgtttc attgaaaaaa tgcaagaaaa agataaagac ttagcagaac cagtcatttc
     6001 aatagaagat aatgctgttc gcgttatgac gatccatgca agtaaaggct tggaattccc
     6061 agttgtattt ttgttagata tgacaaagga gttcaactta caagacttgc gcaatcggta
     6121 tgcttttgaa gaaaaacttg gagcaggaat tcgctatatg gatcctgaaa cacgagtgtt
     6181 atatgatacg ttaccgtttc aagccatcaa attggcaaag caaaacaaat tgttgtcgga
     6241 agaaatgcgt aaattgtatg taggattgac tcgggcagaa caaaagcttt tcattgtcgg
     6301 ttcctataaa aataaagagc aaatgatcca aacgtggtct gaagcagctg atcatgagga
     6361 gttagttttt gatcctgcgc tacgtttgaa gggcagaagc agcttgatga attggattgg
     6421 ttacggattg atccgtcatc ctgaaatgca gaaatactta gaggaagaaa tttcgacttc
     6481 tcttcttcaa cattcaaatg cacagttttc tatttcatgg atgaatcagc aatcgatcat
     6541 tgaacaaaga cagttgcttg ctgaaaaaga actggttaac ttagatcagc agatgaaaga
     6601 agatgaaact ttattagcag attcgttaca aaaacgtctg gcatatgaat atccttatca
     6661 agcttcttca caaaccacaa gttatcaatc tgtatctgaa atcaaacgat tattcgaaga
     6721 tccagatgat acacaagaat cacgtttgac tctggaaagc agtcagaaca aagcagcatc
     6781 ccgtcaattt cgttatacgc aagaacaact agcagaacca aaatttttgc aaaaagatcg
     6841 gcaagtgtca gcagctgctg ttggaactgc tacgcatgct ttgttacaat tactgccatt
     6901 agagatgcca actacagaga gtatccatca aaaattacag gaattggtaa ggaaacggtt
     6961 agtagacgaa aaagtcgcga aaaaagtaga tgtttcttct attatatggt tctttcaaac
     7021 agaacttggt caacagctga ttgccaacaa agaaaacgta aaacgagaac aacctttttc
     7081 catgctgctt cctgctgacg aagtatttca agactatcca aatcaagaag atgaactatt
     7141 aatccacgga atcgtcgatg gatacctaga agaaaaagat catctcaata tctatgattt
     7201 taaaacagat tttattttac atccggatga tccagcagaa atagatgcaa tcgtccaaaa
     7261 atatcaagga cagctaagac tttaccaaca agcaatgtca gaagctttga ataagccagt
     7321 agaaaatgtt ttcctaattt tgcttcgagt gaaacaaatt ataaacatta acaaataatt
     7381 ataattgagc aaacttgact tatgttttgt aaggattatg ctatactctg ggaagaggtg
     7441 agtatgatgg agctaacaca gacagaattc gttttatgcc ctaaatttga aaaaacattc
     7501 tctattttag gaaaaaaatg gaacggattg attatcgatg tactactcga aaacggtccg
     7561 caacgctttg ttgagcttgc ggcaaaaatc ccgatggtca gtgatcgagt attagtcgaa
     7621 cgcttgaagg aattagaaaa agaaggaata gtcactcgaa actttgcctg tagcgaaaag
     7681 agccgttcag aatatctctt gactgaaaaa ggagcagact tgcaaaaagc gatgcgtcaa
     7741 atacaagttt ggagcgaaaa atggatcaca gaggaagaat gcggttgatt ttgtaaaaaa
     7801 aatcttgtaa actatagtcc atacgaataa gtaaagaacg atgacgaaaa agagtagttt
     7861 ggctaaacca gttcagggag atctgtcgca gactgaaagc agatcctgtg tttgaaaaat
     7921 gaagttcact ttccgagttc gtctgataaa atcagaccgg caattgtcgt taacgaagta
     7981 agagtgcggt gtaagtcatt tatccgcaaa ttaggatggt aacgcgaaga cctcgttcct
     8041 taacaggaat gaggtctttt tgttttgtat ttggaattta aaggaggaac aagcacatgt
     8101 ctctacaagc acaattagag agcttgaaag aagcaacgct cgtcaaaatc acagcggcac
     8161 aagatttaaa tgaattaaat caaatacgtg ttgaaacgtt aggaaaaaaa ggaccgatca
     8221 cagaagtatt acgtgggatg aaagatctaa gtccagaaga gcgaccaaaa gtcggaagtt
     8281 tcgcaaatga gatccgagat cttttgacag aagagatcga gcgaaaaaaa caagtattag
     8341 aagaagaagc gttaaatgca gctttggcaa aagaaacaat agacgtgaca ctacctggta
     8401 gaaaagtaaa acatggtaca cgtcatatcg tgacacaaat catggaagaa attgaagata
     8461 tttttgtggg tatgggctat cagatcgtag aaggatatga agtagaatct gatcattaca
     8521 atttcgaacg gatgaatctg ccaaaagatc atccagcaag ggacatgcaa gatactttct
     8581 atatttcaga cgagatattg attcggacac atacttcacc agtccaagca cggacaatgg
     8641 aaaaacatga tttctctaag ggtgctctac gaatgatctc gccagggaaa gtcttccgca
     8701 gagatacaga tgatgcgacc cacagtcatc agttccatca aattgaaggg ctagttgttg
     8761 ataaaaacat cacaatgggc gaccttaaag gaacattaga agtcgtaatg aaaaaaatgt
     8821 ttggggaaga tcgtgaaatt cgtttgcgtc caagttattt cccatttacg gaaccatcag
     8881 tagaagtaga tgtcagctgt ttcaaatgtg gtggtgccgg ttgtaacgta tgtaaataca
     8941 ctggatggat cgaaatttta ggagctggca tggtacaccc gaatgtgttg aagatgtcag
     9001 gaatcgatcc agaagaatat tcaggctttg ctttcggcct tggaccagac cgcgtagcta
     9061 tgttgcggta tggagtgaat gatatccgta atttctatca aaatgactta cgtttcttaa
     9121 gtcagttcaa gggggagtaa gaaaatgtta gtttcatata aatggctcaa tcgttatgta
     9181 gatttatcga atgtaacacc aaaagaatta gcggataaga tgtctgtgac aggaatcgaa
     9241 gtagagggag taacggttcc agaagaaggc ttgaaaaaaa tcgttgtcgg tgaagtcaag
     9301 gaatgcgtac cacatccaga ttctgatcac ctttctattt gtcaagtcga tattggcgaa
     9361 gacgaactgt ctcaaatcgt ttgtggagcg cctaatgtga aggttggaat caaagtgatt
     9421 gttgcacttc ctaactcacg tatcgctggt aatgtaaaaa tcaaaaaagg aaaaatgcgt
     9481 ggccaagtat caaatggcat gatttgctca ttacaagaaa ttggctattc agatagtgtt
     9541 ataccgaaag aatacgctga aggtatctat tatatgccac aagaagctgt aaatggcgat
     9601 ccagtttttc cttatttgga tatggatgat tcgattattg aattatctat cacaccgaat
     9661 cgtgcggatg cattgtcaat gcgtggcgta gcttatgaag ttggggcaat ttatcgtcag
     9721 actcctacat tcgatgacga aaagctggta gaatcctcac gtcctgctag cgataaaatt
     9781 caagtggaag ttgaagataa agaagcagtt cctgcttatc aaatcagaat cattgaagga
     9841 gtaagaatcc agccaagtcc tcaatggctg caaaatcttt tgatgaacga aggcattcgt
     9901 ccaatcaaca atgtagtaga tgtgacgaac tatattttgc ttttattcgg acaaccacta
     9961 catgcatttg actacgacaa attgaaatca aaagagatct ttgtccgccg tgcaaaagaa
    10021 aatgaaacat tagtaacact agatggagaa tctcgggaat tgacaactga caatctcgtc
    10081 attactaacg gcaaagaacc agttgcttta gctggtgtca tgggagggtt agactcagag
    10141 atcacgaatg aaacaacaac agttgcacta gaatctgctt tatttgatcc aatttcgatt
    10201 cgtcgtacag caaaacaatt taatttgcgt agtgaatcct ctgcacgttt tgaaaaagga
    10261 atcaaccatg cgactgttgg agaagccagt gaggttgctg cagcaatgat cgtttctctt
    10321 gcgggtgggg aagttctaca aggcgcggtc aaaggctcag agatcaaagc agaagacgta
    10381 tcagtgaaga tttcacttga acgcatcaat caatatttag gaacatctct tgtcgttaaa
    10441 gaagtaaatg agatttttga agcacttgga tttagctatg aagaaaagga gtatgtttat
    10501 tccgtagtag taccaccaag acgctgggat atccaaatcg aagcagatat tatcgaagaa
    10561 gttgctcgta tttatggata cgaccagttg ccatctacac ttccaagtgg agaaacggtt
    10621 gctggaagct tgtcaaaaga acaagaaact actcgtaaaa ttcgtacaat ctttgaagga
    10681 agcggattga gcgaagcaat cagttatgca ttgacaacag aagaaaaatc aagacagttt
    10741 gttataaatg aaagtaatat tacacgtttg gactggccaa tgagcgaaga acgttctgta
    10801 ttacgaatga atttgatcag cggactatta gatgatatca gttataacac tgcccgaaaa
    10861 aatacagaga ttgctttata tgaaattggt cgggtgttct tccaagaaaa tgatccacgt
    10921 acacatctgc cgcaagaagt aaaacatgca gcaatcgcta tttctggtat ttgggaagaa
    10981 aaagattggc agacgaaagc tcaaaatgta gactttttca ccatcaaggg tcttttagaa
    11041 aacttatttg aacaactagg catcactgaa gatgtccatt atcaaacagt gacagaaatg
    11101 aaggaaatgc atcctggaag aactgctgcg atttaccttg gcgaaaaatt tattggtttt
    11161 gtcggtcaag tgcatccaac gattacaaaa agttatgata ttccagaaac atatgttgct
    11221 gagtttgatt tatctgctat agtggaagct gctcaaaaag ggatcgtttt cgaagcagtg
    11281 acaaaattcc cagcagtcag ccgggacatc gctttgcttg taaaagaaac tgtgacaaat
    11341 caggaattaa cagaagttat ccgttctgct gccggaagat ttttaacaga tatccaatta
    11401 tttgatgtct atcaaggaga aaacattgaa aaaggacata aatccatggc gtatagcctg
    11461 acattcgtca acccagaagc aacattgaca gatgaagaaa tcaataaagc aatggaaaaa
    11521 gtcgaaaaag cgttggtaga aaacctagag gcaagtattc gctaagaagg aaaatatagt
    11581 aagtaacgat caagtagctt ttgaagacca tacatttgaa aaaaagatgt gacacaactt
    11641 cgtcacatct ctttttttga ttttaaaaaa ggaatagcac agagacgaga taaaaaaaca
    11701 ggcctttaca gcctgctttt ttagagtaaa tgaattaatg gaagtaattg attcccattg
    11761 ctgattttac ttcagaaagt gtttgtgcag ctactttagc agcttcttca ctacctttac
    11821 gtaagatttc cataacagct gaaggatctt tttccaattc ttctcggcgc gcacggatcg
    11881 gtgcaaattc agcttccaat acatcaatga ggtatcgttt gatctttaca tcaccaagtc
    11941 cgccgcggcg gtaatgagct ttcatttctt caattgcttc tttatctgtt ccgaatacat
    12001 ccaagtaggt gaagaccata ttcccttcta cttgtccagg atcttgtaca tggatgtggt
    12061 ttggatcagt atacatactc atgacttttt tctgtaagac atcggcagga tcggagatat
    12121 aaataccgtt tcctaacgat ttactcattt tgccatttcc atcgattcca ggtaatctac
    12181 ccattccttt tggtgggaat acgccttttg gttccaccaa gacttcacca tatgtatgat
    12241 tgaagctttg tacgatttcc tgtgtctgct ctagcattgg tttttgatcc tcaccaacag
    12301 gtacaagatt cgctttgaat gcagtgatat ctgctgcttg agaaacagga tagataaaga
    12361 atcctgtagg cacgctttca ccgaattttt tttgttcgat ttctgttttg acagtcggat
    12421 tgcgccgaac gcgtcctacg ctgactaagt tcaaataata catggttaat tcagctaatt
    12481 ccggaatttg tgattggata aataaagtag attttgcagg atctaatccc acggctaaat
    12541 agtctaaagc aacttgcaag acatttgatg aaaccttctc aggattttta gcattatctg
    12601 tcagtgcttg catgtcagca atcatgacaa acagttggtt gtcttcatct gcctgcatag
    12661 ccactcgggt ttttagagaa ccgacgtaat gccctaaatg cagcttccca gttggtcgat
    12721 ctcccgttaa aataatattt ttcaaaaaag acacctcttt acgatttgta agtctgagca
    12781 cttaatatct taccgttttt cctgaaaaga aacaacagtt cctaaggaaa aaacaaggaa
    12841 agaggataaa ataactaata gattagaaat ggaagaaata tttatgggag aataaacgtt
    12901 ctaagaaact ttacaaatta ctaaactatc agatataatg tattttatta tacatcttta
    12961 aaagaaatct tactaaataa taatgggagt gtaattctat ggcaatgatt aaatttgatc
    13021 acgtggataa atattatggc aagttccacg cattaaaaaa tatcaatctc gaattcgaga
    13081 aaggagaagt tgtcgttgtc attggtcctt ctggttcagg aaagagcacg atgcttcgct
    13141 gtatcaatgg attagagacg atctcttcag gtaagctgct gattaatgat actgacttgc
    13201 atgataaaaa gacaaaactg acagaagtcc gaaaaaacat cggcatggtt ttccaacatt
    13261 tcaatttgta cccaaataaa actgttctgg aaaacatcaa attagcacca atcaaagtgt
    13321 tgaagcaaga ccaggcaact gcagtcaaaa acgcggagaa atttttgaaa actgtcaata
    13381 tgttagataa gaaagattct tatccttcca tgttatccgg gggacaacag caacgggtgg
    13441 caatcgccag aggtttagcg atgaatcctg agatgctgct gtttgatgaa cctacttctg
    13501 ctttggatcc ggaaatgatt ggtgatgtat tagacgttat gaaaaaattg gctcatgacg
    13561 gtatgtcaat gattgtcgtg acacatgaaa tgggctttgc caaagaagtg gcggatcgtg
    13621 ttatcttcat ggctgatgga gaagtattgg aagacagcag aaatgtgaga gactttttcg
    13681 aaaatccaaa tgaagtccga gcacagcaat ttatcagtaa agttatcaat cattgatagg
    13741 aggaaaaaga tgcgcaggtt aaaaaaaata cgcctgattt tccttttagg cattgctttt
    13801 cttgcactag ccggatgtaa aggaaatggg ttggcaaatg aagatatttt agagcgaagc
    13861 aaatctacca atgaaatcat ctggggtgtc aagtatgata ctcgattatt tggaatgatg
    13921 gatattgaga gcagaactgt acaaggattt gatgtagata ttgcaaaagc aatcactaaa
    13981 aagatccttg gtgacaatgg aaaaacagaa tttgttgaag tcacttctaa aactcggatt
    14041 ccattattga aaaacggaaa tatcgatgca attattgcaa cgatgacgat cacagatgaa
    14101 cgaaaaaaac aggttgactt ttcagatgtc tatttcgatg ctggccaagc tttgttggta
    14161 aagaaaggaa gccaaatcaa aagtgtagat gatttgaatg cttctacaac ggttctagca
    14221 gtcaaaggtt ctacatcggc tgcgaatatc cgacagcatg caccagatgc aaaaatttta
    14281 gaattagaaa actatgcaga agcatttact gctttacaat ctggtcaggg agacgcgatg
    14341 actacggata atgcgatttt gttagggatt gcagatgaaa atccagagta tgagttagtt
    14401 ggtggaacgt ttactaatga gccttacggg attgcgatca acaaagggca agaaaacttc
    14461 ttgaaagctg ttaaccaagc gctagaggaa atgcatgcag atggcacata cgataaaatc
    14521 tatcaaaaat ggtttccaaa tgaaactgaa ggaaaagtag agtaaggggg agaggcaatg
    14581 agcgaattga tacaaactta tggaccaatc tttgtcgacg ggttcaagat cacattgttt
    14641 tcgagtgtct tagcacttgt gtttagtttg attatcggaa cgttaatggc aatcttccaa
    14701 ttatcaaaga ataaagtagt gagcgcctta gcaaaggcat atgtagaatt ttttagaaac
    14761 attccattat taatcatcgt gatgtttttt tatgttgtcg taccgattta ttggtttaat
    14821 ctagatggat tccaagcagg tacgattgga ttaacgatct atacatctgc ttttattgct
    14881 gaaacagtac gttcagggat tcaaaccgta ccaaaaggtc aaacagaagc aggactatct
    14941 tctggtttta cctattcaga aatcatgcgt tatattgttt taccgcaagc attcaaaatc
    15001 gttatcccgc cattaggcaa ccaatttatc aacctcgtaa aaaactcatc tattttagca
    15061 atcgtagctg gtttagattt gatgtatcaa ggagacttga tcgctagtga aacattcaat
    15121 accttcgata cgtatatcat cgttgggtta ttttacttag ttatcacgct accgttatct
    15181 tacttaatgg tttatcttga gaaaaaatgg gcggttcgtt catagaagga ggggaaatac
    15241 atggatttta gcggcgcttt ttcatggatg aatatccgtt ttttattgga aggattaaag
    15301 gttactgttg aagtttcatt gttttcgatc atttttagtt tcttgatcgg tggtctgaca
    15361 ggtgtgattc gttttgcgaa tatccctttc ttttccaaaa tccttggtgt agtgattgat
    15421 attatacgga atcttccatt attattgatc attttcttca cttactttgc cttacctcaa
    15481 atcggcattc agatgaacat cttctggtcc gctgtcgcag cattaacgat ttttgagtca
    15541 gctatgttgt cggaaatttt ccgagctgga ctcaatgctg ttccaaaagg acagatggaa
    15601 gcaggattat caacgggact gacttatgta gaatcgatgc gaacgatcgt gttaccgcaa
    15661 gcatttaaat caatgatccc ggcaattgtg agccaattga tttcactgat caaagatact
    15721 tcattagcag taatcatttc tttacctgaa ctttctcatc aggcacgtat tatttatgga
    15781 cagaacacca attatgtgct tccgatgttc atcatcatga cacttatgta cttcgtggta
    15841 tgttacgcgc tttctctgct ttccggttac ttggaaaaga gacggtacag ttattaatag
    15901 atgattcttt tagcggttgt ggcaaactaa agtcacaacc gcttttcctc tttccattcg
    15961 aaaaaaagag atttcgattg ttttaagcaa gtaagagcac ttgaaaattc acgctaaaaa
    16021 gtacacaata gggaggatag aatttattga aaaaaaggat gatacgattg acagataatc
    16081 gccctatcgg atttattgat tcaggtgtcg gcggcttgac tgtagtaaaa gaagccctga
    16141 aacaattacc gaatgaaaat attttatttg taggagacac agcacgctgc ccatatggcc
    16201 ctagacccgc ggaacaggta atccagtata cttgggaaat gacggattat ctggtggagc
    16261 aaggaatcaa gatgctggtg atcgcctgca ataccgcaac tgcggtggct ttagaagaaa
    16321 tcaaagctgc tctttctatt ccagtcatcg gtgtgatcct tcccggtact agagcggcag
    16381 taaaaaaaac acaaaataaa caagttggca ttatcggtac gattggtacg gtaaaaagtc
    16441 aagcttatga aaaagcactg aaagagaaag taccagaatt gactgtgaca agtcttgctt
    16501 gtcccaaatt tgtttcagtt gtcgaaagta atgaatacca ttcatcggtg gcgaaaaaaa
    16561 ttgtggcaga aacattagct cctttaacca ctaaaaaaat cgatacattg attttgggat
    16621 gcacccatta tccattatta cgccccatca ttcaaaatgt aatgggagaa aatgttcaac
    16681 tgatcgattc tggagcagaa acagtaggtg aagtatctat gctgttagat tatttcaatt
    16741 tgagcaattc accgcaaaat ggtcggacat tatgccagtt ttatacaact ggctctgcca
    16801 aacttttcga ggaaatagct gaagactggc ttggaatcgg acacttaaat gtagaacata
    16861 tcgaattggg aggaaaataa ggatgcgtca tgacgggaga aaagcccaag agcttcgcaa
    16921 actggagctt caaacaaatg ttttcaaata tccagaagga tcggtagtga tccgttttgg
    16981 tgatacaaca gttatttgtt ctgcaacaat agaaaattct gttccccctt ttttgagaga
    17041 aacaggaaca ggatgggtga cagcagaata tagtatgctg cctcgagcaa cgagtacgag
    17101 aaatcgtaga gaaagcagca aagggaaact ttctggtaga acaatggaaa tccagcgtct
    17161 tattggtcgt tccttgcgtg cagtagtaga cttggaaaaa ttaggagaac gtagtatcat
    17221 cgtagactgc gatgtgatcc aagcggatgg cggtactaga acagcaagta tcactggggc
    17281 atttgtggca ttgaaattag ccattgaaaa acttttaaga gaaaaagaat tgtcagaaga
    17341 tccaataaaa gaacatcttg ctgcagttag cgtaggaatc ttaccagatg ggacttgcgt
    17401 cacagatttg gattaccaag aagattccgc agcacttgta gacatgaatc ttgtaatgac
    17461 agaatctgga aaattcgtgg agattcaagg aactggagaa gaagcaacgt ttgatggtga
    17521 acagttgaat gaaatgctct tttttggaaa aaacgcgatt gaagatttga tcaaagagca
    17581 aaaacatgct ttgctaacag agtttgctca aaatgatgag cggatagaag aaacaaaaac
    17641 aatcatcatt gcgacaagaa atccaggaaa agcagaagag tttcgaaata tgttcaaaga
    17701 agcgggatat catgtaaaaa cattacttga ctatcctgaa ttgccagatg tggaagaaac
    17761 tggcagcaca tttgaagaaa atgcccgatt aaaagcagaa acgattgcac aattgcttga
    17821 tcagccagtc ttagcagatg attcaggttt gaaagtggat gctttaggcg gtatgccagg
    17881 aatctattct gcacgttttg caggggaaca aaaaagtgat gcggggaata acgccaaatt
    17941 attatatgaa ttgacagatg tgccggatga aaaaagaaca gcacaattcc attgtacgtt
    18001 agtattcgcg gcacccaaaa aagacagcct tgtagtagaa gcggaatggc ccggtcgagt
    18061 agcccgtata ccatctggcg aaaatggctt tgggtatgat cctttattta tcccagaagg
    18121 taagaaacaa acagcagcag aattatcgag cgaagaaaaa aacaagatca gtcatcgggc
    18181 tcaagcaatg aaaaaattga gtgccgagtg gaaacaatgg ctggaaggag agagatagat
    18241 gcggtattta gtagtaagtg ataatcatgg agacagagat atcctcgttg acttagtaga
    18301 tcgttatcgt tctgaagtgg atatcttttt ccattgtggt gattcggaat tagaagcttc
    18361 tgacccatta tgggatacat tccaagtggt tcaaggtaat tgtgattatg gaccaggatt
    18421 cgaacagaaa aaagtgatcc aaactggtca ggatacagta ttcatgactc acggtcattt
    18481 atcgaatgtc cgatttggtt tgacacagtt agcaatcgaa gctcgagctg caactgccga
    18541 tatggtgttt tttggtcata cccatcaaat aggctgtgag atggatcaga aaatcctgtt
    18601 tttgaatcct ggtagcattt ctcagcctag aggcccgatc caaatcccat catatgctct
    18661 tatcgaaagt ttagatgacc aaatttctgt tcaatactat aaccgtgccc atcaggcagt
    18721 tgaagagttg gccttttctt tttcgagaga gcgctgaata aaaaaactct aatttttaat
    18781 aaaaataagc tttattttat gaattttgca tccggtttcg gtaaaatagt agtgttgaat
    18841 gaaatagaaa tggaggccat cagatgattg gacctatagt aagagaaatg ctgctggaaa
    18901 atcaggaaac gtttttgatt cctgcagaaa atgtggcaaa tgttatgtat catcatccat
    18961 tgtcccatgg tttacttgtg ttatcgaagg tcggttattc caaaattcct gtattaggaa
    19021 aagatgatca ctttgtagga ctagtaagtt tatcagatgt ggtcaataaa atgattgact
    19081 tgcaaacaat cagcatggac ccgttagaag gactaacggt tgctgatgtg acagaaacag
    19141 atgttccgac aattgatgaa aattgggaac tagaagaagt actccatttg ttagtcgatt
    19201 cgcctttttt accggttgtc acagaagatc ggattttcaa aggaatcatt acaagaaaag
    19261 agctgcttaa agccgtaaac ttcatggtgc atgaattaga acgtcgtaat atattgtctc
    19321 cgaaaatcga agaattaaaa gaacaaatcg atatcgtgag ctaaagaacg agaaactaaa
    19381 agataatcaa aaaataagta gaaaaaacag cgtaccagtt ttgactttat cttgaaaaag
    19441 aggctgggac aaaagcgttt aactccaaga aataagaagg aattcacgaa aattgctttt
    19501 caaatttttg tgaatttcag cttatttccg aaggagttgc ttctgcttcc gccgtttata
    19561 cgtgtttaga gcgtgggaca aaagtgttct ttacttttgt cccacgctct ttttagtgag
    19621 attcgttgca tcgtttgtcc aaattatttt ccgacagcaa ttggactgtt cggaatcgtg
    19681 aaaccagaag ctgtcaattc tttgacatag gagctcaaga actcttcttt taaggcatat
    19741 tgttttccgt tcaatgcgta gcagaccgta cgaatcgcaa aattgctgtt tccaagatcc
    19801 actaatccaa agatagaagg ttcagtttgg agctcttctt ggtatttttc agctaatgta
    19861 cgatttactc gatcaatgat ctcatagatt ttgtcatatc cttcttcagg aacaatccga
    19921 atatctaaaa gtacttgcat atttgcacga gagagattgc tgatcgtggt gatgtttcga
    19981 ttcgggatga aatggacagt accatccgtt gctttcagct gaagggtacg gataccaaca
    20041 gatacgacgg ttccttcaat cgttaagttt gctagcttga tgtagtcccc aacatccatc
    20101 tgttgttcca tgataataaa aaatccggta ataacatcac tcatgaaccc ttgggcacca
    20161 agaccgattg ccacacctgc aattccggca ccagccaaaa gtgatccgat cggcacaccg
    20221 atagcagata aaattgcata gataaaaaag aaaccaaggg tataatgaaa aatagtattt
    20281 aaaagactat gtaaagtctt taagcggctc tcgtttagaa tggctttttt agaatactga
    20341 ttaaaagact tatcaatcag atattttccc acacggtgaa gaatccaaaa aaggacgatc
    20401 aaaaaaatca gatataaact tttttggata aaggcagcca aaatggcgtc ccaatcaatc
    20461 ccgtcccaaa accgctgaaa cgcatttact ttgctgacag cttgatctgt caatgtctcg
    20521 ccagaagagt ctgtcgtacc ggcagttgct aataatgaaa acataaaatc ctcttttcta
    20581 aaatttgatt ctttctgtat tgtaccaaga agttttcaaa aaaggaaata gcaaaaataa
    20641 ctataatgaa ggaaaaaagc tgaaaataaa atgacaaata atatggataa tgataaaatg
    20701 tggagaaaat aacgaaggtg gtaagtatat gcaaatcgat tggaacaatc taggtttttc
    20761 ttatatcaaa actccttggc gttttattgc caaatggaaa gacggacaat gggaagaagg
    20821 agaattgaca gaagataatt acctgacgat tcatgaaggc tctccagcgc ttcattatgg
    20881 tcagcaatgt tttgaaggtc taaaagctta tcgtcgaaaa gacggcaagg tcaatctctt
    20941 tcgacctgaa caaaatagtc gacggatgaa tcaaagtgct agacgattgc tgatgccaat
    21001 ggtccctgaa aagatgttcg ttgatgctgt caagcaagtg gtaaaagcaa atgaagattt
    21061 tgtgccgccc tatggaacag gcgctacatt atacttgcgc cctttactga ttggggtcgg
    21121 tgaaaacatt ggtgtccatc cagctccaga atatttattt gtcgtcttct gtacgccagt
    21181 aggagcgtat ttcaaaggtg gactaaaacc aacgaatttt attgtatctg attacgatcg
    21241 tgctgctcct aaggggactg gtgcagcaaa agtcggcgga aattacgcag cgagtttact
    21301 tccaggaaca gaagcaaaag aactggatta ctcagattgt gtttatcttg atccagccac
    21361 acatacgaaa atcgaagaag taggtgcagc aaatttcttt ggaattacaa aagacggaaa
    21421 attcatcact ccgcaatcgc cctctatctt accaagtatc acgaaatatt ccttgttgca
    21481 gttagctgaa gaacgactag gactgcaagc aattgaagga gatattttca ttgatgatct
    21541 agatcaattt gcagaagctg gtgcatgtgg tacagcggct gttatttctc caatcggtgg
    21601 gatttatcac aataacaatc ttcatgtatt ttatagtgaa acggaagtag gaccagtaac
    21661 aaaacggttg tatgatgaat tgacaggtat tcaatttgga gacatagaag caccagaagg
    21721 ctggattgaa gtcgtggaat aatttcaaag taatgagttg aaaaaaacgt aaaaagaaaa
    21781 aatcaggaag agactttaaa aagttttttc ctgattttta tatgttttac tttaaagaat
    21841 gcaacaaaag tttatgaaag atggacacct ttgtcaacgt agataatatc accgatgatt
    21901 ccgctggaaa gtgggctgat taagaaagca caagcattac cgacttcttc gatcgtcaca
    21961 ccttgctgat caggcgtacg agattcagat aactggatca gtttttgata gtctttaaca
    22021 cctgtgacag ctaatgtttt gattgcccca gctgagattc cattgacccg aattccttta
    22081 ggcgagaact cagctgccaa ataacggata gcagcttcaa gagaagcttt cgcaataccc
    22141 atcatgttat aattcgggat tgcgcgttct gaacctagat aagtcatgct aacgatcccg
    22201 ctgtttggtg caagaaacgg ttgtgcgtat tttgatacag caattaaaga gtagctgctg
    22261 atatcttgag ctaattgata gccgtctctg gaaatatcag acacatttcc ggacaattct
    22321 tctttgttgg cgtaagcaac tgcatggaca aggccgtgga tctccccagc atattcaccg
    22381 atggcattca ttgctttttc gatgctttcg tcgctagaaa catcacattc tacagtgaat
    22441 gcttcttcgc cgaccaattt gactaatgat tttttcattc gttcattttg gtaagtatag
    22501 ataatttctg ccccttgttt caacattgct tcagcacaac cccaagcaat gctttttttg
    22561 ttagcgacgc ccataatgac gatttttttt ccttctagaa atgacataat gactctcctt
    22621 tgtttctgat aaaaatttga tagtcgtaaa aaaatagaga ataatagcta aaataacttc
    22681 ttgatttctt aattattagt caaaatgttt tgattgtcaa actatttttt cttaaattaa
    22741 aaaaagattg ctaaatattt aggaatatga tactttgata gtcaaagtaa tgaaaatggc
    22801 aattgcctga aagaaggagt ttaattatgt cttacgtatt atctgctact gaagtaatgg
    22861 aattgattcc taatcgttat cctatttgtt atattgatta tgtagatact ttagaaccag
    22921 gaaaaaagat cgttgcgaca aaaaatgtaa caatcaatga agattttttc caaggtcact
    22981 ttcctgacaa tccagtgatg ccgggtgcgt tgattctaga aacactggca caagcaggtt
    23041 cgatacttat cctaaaatca gaagagtttc taggaaaaag agcttatatc ggtgggatca
    23101 ataaggccaa attccgtcaa aaagttgttc caggagatgt gctgaaatgt cattttgaaa
    23161 tcaccaaaat gaagggatca gtcggtacag cgttatctgc agcatatgta gatgataaaa
    23221 aagtctgtga gtgcgagttc acgttcatcg tcaaccaaaa tgcttgacta taaaacctga
    23281 tatagcaaaa caaaaggaag cttacttgct ttttatgtaa aaagaagacg cctgaatcct
    23341 agcatacgtt gtcatctatg caggattcag acgttatttt tttatttttg ttttgcttct
    23401 tgcagtatat tgattttatc gatttttact tcttcttttg gtttgtctga actatctgtt
    23461 tcgatatttg caatagcatc cacgacatcc attccttcaa tcacttggcc gaagacagta
    23521 taatggccat ctagtgaagg ataaccgccg tttttataag cgtcgatgat tgcttgagga
    23581 tagtcgtctt ttaaaagacc atcatgcata tcatctgtat tttgaacgat aaagaactgg
    23641 ctgccgttac tgttaggatc ttgtgagcga gccatagata acgcacctct tatattataa
    23701 aggtggttgt tgatctctgt ttcaaatcct tctccccaaa tgctttcccc tccagtgcca
    23761 tcgcctttag gatcgccacc ttggatcatg aaatctttga tgacgcgatg gaaagtcagt
    23821 ccgttgtagt aaccgtcttt tgcatgtgtc ataaagtttt caaccgcttt tggtgcgtgt
    23881 tcgggaaata atttgatttt gattgtgcct ttattcgtca ccatttcgac cagatcttca
    23941 ttttcggcga catctttcgt caattgtggt aattccaatg cgttcaaatc gacgctactg
    24001 gcagtcgtgc tttccgcagt agatgtactt tttgaacttt ctgtagacga actagaagaa
    24061 gtcgtgttgt ttgcagaaca accagcagca aacagcaatg ctgctgctaa actaatactt
    24121 aaaataactt tatttttctt cataactgtt cctttccaat ttactatttt gttatcttta
    24181 atgataacaa aatagaggga aaatacctac ttaaattatt ggaatttatc atttatttta
    24241 aaagaaaaag aaaaggtgta gaccatttac tttgtgtttg ctataatgta agtggataca
    24301 aaaacgaaaa gatgcaggag gaaatggtaa tatgggaaaa ttaggaatct cgatttatcc
    24361 agaacggtca acatttgaaa aggacaaagc gtacttggat ttagctcata agtatgggta
    24421 taaacgggtt tttaccagtt tgctgcagat caatgatgat aaagaaaaag ttctttcaga
    24481 atttaaagaa gtagttgatt acgcgaatag cctaggcatg gaagtaatgg ttgatatcaa
    24541 tccagcgtta tttgaacagt tggagatttc ttacgatgac ttatcttttt tccataagat
    24601 gggtgcctac ggggtccgct tagatatcgg attcacagga gcagaagaag caaaaatgac
    24661 gcgcaatcct tttggtatca aaatcgagat caatatgagt tcgggcacga attacgttga
    24721 caacatcatg agttattcgc caaacacgga taatttatta ggttctcata atttttatcc
    24781 tcatcgatac tctggtttag gatacgaaca ctttgttttt tgttcagaaa aattcagaaa
    24841 atataatttg aatacgatgg cattcgtcaa ttcgcaatct gctgagtttg gcccttggcc
    24901 tacgcaagat ggcttgtgca cgctggaaga ccaccgagat cttgaaattg cgacacaagt
    24961 gaagcattta atccttacag gattgattga tgatatttct attggaaatg cgtacgcaag
    25021 tgaagaagaa ttgaaagaaa tggcagaagc atttaacgcg gattatccaa cattgaaagt
    25081 agatacggaa gaaggtatta cagaaaacga acgaatctgc ttgtttgata accttcatag
    25141 ttatcgaggg gatcgctcag aatatatcct tcgttcaacg atgactcgtg tatactacaa
    25201 agataaagat ttcccgcctc ataatacgcg agatatgcat catggggatg tactgattga
    25261 caatgaaggg tacggtcaat acaaaggaga aacacaaatc gccttgaagg atatgaaaaa
    25321 cgacggacgt gtcaatgttg taggaagaat ctccgatgac gaattattcc tccttgactt
    25381 tttgaaacca tggtcaagct ttaaattaat tgaaaataaa aaatagataa ttagttattg
    25441 cagaaataat ccgctagaaa tagccagtta tttctgcttt tttattttgt ttttttatag
//
LOCUS       DACDKM010000025        25468 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20834_2016
            SAMN12640370-rid9891663.denovo.025, whole genome shotgun sequence.
ACCESSION   DACDKM010000025 DACDKM010000000
VERSION     DACDKM010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640370
            Sequence Read Archive: SRR10040891
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 25468)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25468)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742835.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742835.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:04:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,012
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 29 of 75
            Pseudo Genes (incomplete)         :: 33 of 75
            Pseudo Genes (internal stop)      :: 19 of 75
            Pseudo Genes (multiple problems)  :: 5 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25468
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640370-rid9891663.denovo.025"
                     /isolate="E20834_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            595..1923
                     /locus_tag="IV334_001059"
     CDS             595..1923
                     /locus_tag="IV334_001059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HAP8943148.1"
                     /translation="MKAITPDMTTKFNKQFQANIKQQALQRAVVKNGITASAENVSAH
                     VENTPVFSIDLATGKVANQMQSGRCWMFAALNTFRHKMLNQFHLKEFELSQNYTFFWD
                     KYEKANYFYENIIATGKEAIDSRKVAFLLATPQQDGGQWDMIVSLFQKYGVVPKSVMP
                     ESANSSNSRDLNNYLNKLLRKDAVVLRKMVAQGETLDAIEEKKEEMLESIYNFLAISL
                     GTPPKEFDFEYRDEEKNYHLDRGLTPQIFYDKYIGVKLDDYVSVINAPTKDKPFNRSY
                     TVEMLGNVVGGKEVKYLNVDMETFKKLAIAQLEEGESVWFGCDVGQSSTRTSGIMALD
                     AYSMDDLFDTDFTMTKAERLDFGESLMTHAMVLTGVDLVDGQSTKWKVENSWGEKVGK
                     NGFFVMSDAWMDEYTYQIVVRKDLLTKEQLNAFNEEPIVLSPWDPMGALA"
     gene            2125..2958
                     /locus_tag="IV334_001060"
     CDS             2125..2958
                     /locus_tag="IV334_001060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructosamine kinase family protein"
                     /protein_id="HAP8943149.1"
                     /translation="MDIQTVLSDLKLNGKVIPVVGGDVNQTYRIKTEHRAYFLKIHPN
                     VKKGFFEAEVDGLKELSAFVRVPDTYMLGETSEGAYLLMEWIEPGKGDQRDLAAALAN
                     LHQQTAPQFGFRKDNYLGTLVQKNSFEEDWWTFFFKDRLESQISLAEETNRWNVQRQE
                     KYLRFKERVLKSVEPKKITPRLLHGDLWSGNVFFDQQGHPVFVDPAVSYGNREQDIAM
                     SQLFGGFRPEFLDAYQTIFPLEKGWKDRLPIYQLYYLLAHLNMFGESYGSQVDQLLEN
                     F"
     gene            2974..3711
                     /locus_tag="IV334_001061"
     CDS             2974..3711
                     /locus_tag="IV334_001061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-[acyl-carrier-protein] thioesterase"
                     /protein_id="HAP8943150.1"
                     /translation="MAKKYTKKHEVSYYECDINQTMTFPSMLGVVIKTSEDQSDQLGR
                     GSEFVNSFGLTWVITNYSMEINRLPRVGEVISVTTQAMEYNKFFCYRNFWIHDEDGKE
                     LVKIESVFVLMDFVNRKMSSVNEEIIAPFESEKIKKIKRQEKIEKIDSGFMLPYRVRF
                     YDIDSNQHVNNAMYFNWIIDVLGYDFLTTHVPEKVIIRFDKEVEYGNEIESHFEQIPI
                     ENGVKTKHEIRMQEQLYCEANIIWKNK"
     gene            complement(3866..4792)
                     /locus_tag="IV334_001062"
     CDS             complement(3866..4792)
                     /locus_tag="IV334_001062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HAP8943151.1"
                     /translation="MKENMLTGGVSPDVANPLLDAEITIPKHVELLVEDIFNLPSPHI
                     MPSHMLVLKERILSAYASGISGVVITHGTDTLEETAFFLDTTIGNKLPIVLTGAMRSS
                     NELGSDGLYNFESAIRVASCEEALDKGVLVVMNDEIHSARYVTKTHTTNVATFRTPTL
                     GPIGLVTKNRILFLQELLETKRLDISAVDGTIPIVKAYAGMQGDLLEAIAHTKVDGLV
                     IEALGAGNLPPQALAALEKLLVQKIPVVLVSRCFNGIAEPVYDYEGGGKELEKMGIIF
                     CNSINSQKARLKLLIAVNYGLSGEELISFIQN"
     gene            5067..5900
                     /locus_tag="IV334_001063"
     CDS             5067..5900
                     /locus_tag="IV334_001063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HAP8943152.1"
                     /translation="MIRIGLTSFKEHDKLTGKKNNTLYEYAGFLPLVELDTAYYGIPR
                     KSSVENWISEVPESFRFVVKVYQGISGQGDWKPYYQTEEEMIAHFLDVMSPMVDAGKM
                     FCFLVQFPSSFKCTKENVQYLRKLRKWFEGYPVSIELRDYSWYSDEFLERTRNFMSRA
                     AFSLAIIDEPQLLNTTIPFDPFVTNPNFALFRFHGRNAQGWQANDKEWRKKRTLYRYS
                     EKELQELAHEVKVVDSKTKEVGVIFNNNSGGDAAENALALQKILNIKPDGLNPTQMDL
                     F"
     gene            5934..6773
                     /locus_tag="IV334_001064"
     CDS             5934..6773
                     /locus_tag="IV334_001064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAP8943153.1"
                     /translation="MIKAIFFDIDGTLVTNRSKALESTKQAIEYARKNGILCGVATGR
                     SPVKIKEIIDELELDMYVVYNGQLVFTADRTIIDHPFEQKVLEHIVEFADENHRQIVF
                     GARNRLDGSTTMLLGQSIFIKRLVSFLPRKFPVRLMKKILQVFSPHRQKDRYETLEIL
                     KEPIYQCILLSPESEQKKLEHRFPECTFQRSNTYTVDIIPKGGSKLLGIQAFANAVEI
                     EMEEIMAFGDHYNDIEMLKGVGIGVAMGNAQIEVKHAADFVTQSNEEDGIYEGLKHFG
                     LIY"
     gene            6799..7359
                     /locus_tag="IV334_001065"
     CDS             6799..7359
                     /locus_tag="IV334_001065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HAP8943154.1"
                     /translation="MEAETPFQKVETFHKTFDPRRPEIPTAFTENEAHTRAAFKIEEL
                     VEFVAATSPNKEQLHEAVMLLHEALDKAEEKILGKTQWGSDPLVEQVDALTDILYFTY
                     GSFSLLGIVPDRIFEIVHQANMGKLFPDGKPHYDPVTNKVLKPENWQMEYAPEPKIAA
                     EIQRQIQEAIAKKKSADIGMCTNEKE"
     gene            7558..9279
                     /locus_tag="IV334_001066"
     CDS             7558..9279
                     /locus_tag="IV334_001066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation ring formation regulator EzrA"
                     /protein_id="HAP8943155.1"
                     /translation="MGNNVIIGIVIAIIIVAIVVFLVSYLMKKKNQDRLDALEKRKEE
                     LFDLPVIEEVDDVKKMHLVGQSQNTFREWSQKWNDISTSSFAELESQIFEVENLNESF
                     RFLKGKKAVVEAEATMDQMEKQVTEIREGLKELRETEERNSLAVQQALDVYEELKEKV
                     REDEHEYGPALFELQKQIKVIEKEFTQFVTLNTSGDPVEAREVLDKAEKHTYEVEALM
                     KKIPPLYEELHTTFPEQLEEISDTYEKLMEEQYVFPEEDLAEDIAKVSRRIENSLANL
                     EKTEVETVEFENRETADLIDSLYDILEREMEAQRYVKTNQSTIAEYIKHTTKNNRQLL
                     IELDHTSQSYTLNHNEIGRVRGFQTEVEEMERQNEQMIPQIRQHEIPYSEVRTFYKTV
                     FKVLEDIEKQQVEIDDSLHELRKGEKEAQEKIDTFEFKLRSLKRFVEKQRLPGLPNDY
                     LEFFFVATDRIEELSFVLNKIRVNMEEVNRLVALCEEDLELLDKKTHDLVDAAALTEQ
                     MLQYANRYRHTHEEVREAIDKSYYLFNKEYHYQEALDEIGTALERVEPGAFKRIEDFY
                     FNHPDLV"
     gene            9444..10586
                     /locus_tag="IV334_001067"
     CDS             9444..10586
                     /locus_tag="IV334_001067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HAP8943156.1"
                     /translation="MIYFDNSATTPIFPQSLDAYVKTSQRIIGNPSSLHDLGNQANRL
                     LQQARKQIADLIHVEPEEIYFTSGGTEGDNWVLKGTMIEKREYGNHMIISGVEHPAVS
                     ETAEQLKELGIEVSIAPVDKRGFVRVDELKELIRKETVLVSVMAVNNEVGSIQPIQEI
                     SDLLDDFPKIHFHVDAVQAIGKVPFSQWLTERVDFATFSAHKFHGPRGTGFIYWKKGR
                     RLAPLLTGGGQERNQRSGTENVPGIVAMVKALRLSLEIKEQKPEQTQRLKVALINALK
                     TYEKVTIFSEGTDYAPHILCFALKGIRGEVLVHALEEKQIFVSTTSACSSRKHMASST
                     LHAMHVPNDLATSAVRISLDENNSLAEVEQFMIIFKQLYKKFSAIN"
     gene            10602..11813
                     /gene="thiI"
                     /locus_tag="IV334_001068"
     CDS             10602..11813
                     /gene="thiI"
                     /locus_tag="IV334_001068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 4-thiouridine(8) synthase ThiI"
                     /protein_id="HAP8943157.1"
                     /translation="MKYSEIMVRYGELSTKGKNRKSFIMQLAQNVRQALKDFPEIKIH
                     ADRDRMHLLLNGADGQLVIPKLAKIFGIQNFSPSIRVEKDVEVLKQAVQDIMKEIYTG
                     KETFKIIAKRSDHQFELTSNELNQTLGNAVFDIFPHIQVQMRQPDIPLRVEIRRDGAY
                     LSYETIKGAGGLPVGTSGKGMLMLSGGIDSPVAGYFAMKRGVEIEAVHFASPPYTSEQ
                     ALQKAKDLTAKLAPYVGTIHFIEVPFTEIQEEIKKSVPQGYWMTITRRMMLRLTDEIR
                     RIRHGLVILNGESLGQVASQTLQSMVAINEVTNTPIIRPVATMDKLEIIEVAEQIDTF
                     NLSIQPFEDCCTIFAPPQPKTRPQLEKVHQYEERLAIDEMIQRALAGLKMEEIQAESS
                     NEKTEEFSELL"
     gene            12386..13033
                     /locus_tag="IV334_001069"
     CDS             12386..13033
                     /locus_tag="IV334_001069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HAP8943158.1"
                     /translation="MREKTDTLPKATAKRLPLYLRYLKMLDDSGISRIKSNEFSEITQ
                     IPSATIRRDFSQLGELGRSGYGYDVPFLIDVFNNILNTKEEKRIALVGYGNLGKALKH
                     NNFRRNENLNIVCVFDNDPALINRVIDGEMIYPIDRFAEIAKAKNVTVAISTVPSKYS
                     QSAIDEIVKGNVTAILNFAPDRVTVPAYVNVQYIDLTTELQTLIYFDENYSEVFS"
     gene            13426..16071
                     /locus_tag="IV334_001070"
     CDS             13426..16071
                     /locus_tag="IV334_001070"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HAP8943159.1"
                     /translation="MTEENNLSTKYNPQEVEAGRYQHWLEQDLFKPSGDKKAKPYSIV
                     IPPPNVTGKLHLGHAWDTTLQDMLIRQKRMQGYDTLWLPGMDHAGIATQAKVEAKLRE
                     QGISRYDLGREKFIDQVWEWKEEYASHIREQWAKMGLSLDYSRERFTLDDGLSEAVRK
                     VFVTLYEKGLIYRGEYIINWDPQARTALSDIEVIHKDIEGAFYHMSYELADGSGVVEI
                     ATTRPETMLGDTAIAVHPEDERYQALIGKKVILPLVNKQIPIIADEYVDMEFGTGVVK
                     ITPAHDPNDFEVGNRHDLPRVNVMDDDGTMNELAGKYAGMERFAARKAIVQDLKEIGR
                     LIKIDTMIHSVGHSERTGVVVEPRLSTQWFVKMAPLAKKAIDNQETDQAVEFFPPRFN
                     QTFLRWMENVHDWVISRQLWWGHQIPAWYHKETGEMYVGMEAPADSENWVQDPDVLDT
                     WFSSALWPFSTMGWPDTESEDYKRYFPTSTLVTGYDIIFFWVSRMIFQSLEFTGERPF
                     ENVLIHGLIRDEQGRKMSKSLGNGIDPMEVIEKYGADALRWFLSNGSAPGQDVRFSYE
                     KMDASWNFINKIWNASRFVIMNIEGMTVEEIDLSGEKSVADRWILTRLNETIERVTEL
                     FDRFEFGEAGRQLYNFIWDDFCDWYIEMSKEVLYGDDAVSKQTTRSILVHTLDQILRL
                     LHPIMPFVTEEIWEHIPHQGNSLVVAEYPVVRPEFDDETASKGMEVLKELIRAVRNIR
                     SEVNTPLSKPITLLIKTNDAKIDQFLVENTSYIERFCNPEELTISSEITAPDLAMSAV
                     LTGAEIFLPLAGLINIEEEIKRLEKELEKWTSEVKRVQGKLSNERFVSNAPEEVVEAE
                     RAKEKDYLEKQTAVKERIESLRTIQ"
     gene            16381..17694
                     /locus_tag="IV334_001071"
     CDS             16381..17694
                     /locus_tag="IV334_001071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional folylpolyglutamate
                     synthase/dihydrofolate synthase"
                     /protein_id="HAP8943160.1"
                     /translation="MTIEEAIEWIHSRLPFGSRPGLDRVEALLEVVGHPERKVKTIHI
                     AGTNGKGSTVAYLRSLLEEAGLTVGTFTSPYIETFNERIAINGKSIPDNDLIQLVKKY
                     QPLIKQLDQFEAVAGITEFETLTAMAFDYFLMKKVDVAIIEVGLGGLLDSTNVAVPML
                     TGITTIGLDHTEILGDTIEEIAAQKAGIIKQGVPVVTGNIAPEAFTVIQQTAKAKNAP
                     LYVYGENYKINYLHPDEMWGEVFEFFNEIGKIPQLTTPLLGQHQAENAGVAIELYFVY
                     CQLEKLPFREKDVLKGLKKSRWPGRMERLSNEPLVVLDGAHNTHAMNRLVENLKKEFK
                     GYQINILFSALRTKNVREMLERLQQVPNVHIYLTTFEHPKAIELSEVSDLASERVSIV
                     SLWQFGLADILEKMTNEDVLVVTGSLYFISEVRHLLIQLGGDHEV"
     gene            17684..18337
                     /locus_tag="IV334_001072"
     CDS             17684..18337
                     /locus_tag="IV334_001072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HAP8943161.1"
                     /translation="MKYNAVIFDMDGLLFDTELVYYEATQVVADKMGFPYDKDLYLKF
                     LGVSDEEVWANYHMIFSEFGKTSVQRFIDDSYQETLERFSQGAVQMKPGVLELLSFLE
                     EHQIPKVVASSNQRAVIELLLKKNNLFERFETIVSAENVQRAKPDPEIFLLANQKLGT
                     AKKETLILEDSQNGILAASAAEIPVVMVPDLLPPSQELIKKTAGVVSSLHEIPALLK"
     gene            18392..18943
                     /gene="radC"
                     /locus_tag="IV334_001073"
                     /pseudo
     CDS             18392..18943
                     /gene="radC"
                     /locus_tag="IV334_001073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736077.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
     gene            19074..20159
                     /locus_tag="IV334_001074"
     CDS             19074..20159
                     /locus_tag="IV334_001074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HAP8943162.1"
                     /translation="MKVQRIEVENKPYPLYLLLDKEYQLIEPVMKFIKYLDNTGKSPN
                     TIKAYCYHLKLLYEFMEQRGVILNDINFELLADFVGWLRYPSASNVIDLQSKKAIREE
                     TTVNTILNVVMSFLDYLSRLGEFKSIDVFKQAKGRNFKGFLHHVNKGRYQKNVLKLRV
                     KKKQIRTLRSEEVKQIIDACHTKRDKLILMLMYEGGLRIGEVLSLRLEDIVTWDNQIH
                     LTPRDVNVNEAYIKLRKERTIHVSKELMSLYTDYLIYEYSEELEHDYVFISLKEGYFG
                     KPLKYQSVLDLVRRIVKRTGIEFTSHMLRHTHATQLIREGWDVAFVQKRLGHAHVQTT
                     LNTYVHLSDQDMKNEFNKYLERKEHKK"
     gene            20156..22048
                     /locus_tag="IV334_001075"
     CDS             20156..22048
                     /locus_tag="IV334_001075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001833801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HAP8943163.1"
                     /translation="MNASSKRKIISQSEISKKIAVMNEEMQGFWANNSWDIRKCPHPS
                     AIELSKNPALRNRWVRFERVKNLWLRTELKYFYFYHLNNGIWNAKTVWIRKGTVINKM
                     LDFLDLKYPSITSITEVPIEKAMTEYRTYLTKRGVRITTTNYKITANQEKTPVKANSY
                     YVTNLKQFMEFYENFYFDGEEWDKDVWDRRNLPLPDDKVNPTQYEYTINFKGFRNTYF
                     KQLVKRYCKLRLNMDSFSYVSDIAQRLKEFFNFLDMKFKQVQRVHQLTRVEIEAYLSE
                     LNMMGIKPSTITGRISILEGLFSTLLRLEWDDVPSKILIYSEDYPKIPRAKPRFIDEF
                     VLEQLNSHLDKLPEYIATMTMIVQECGMRISELCTLKKGCLLEDKDGDFFLKYYQWKM
                     KKEHIVPISKEVALLIKVREDKVSEEFPDSEYLFPRKDGSPLKQETFRGELNKLAYEQ
                     NIVDKSGEIYRFHAHAFRHTVGTRMINNGMPQHIVQKFLGHESPEMTSRYAHIFDETL
                     KNEFTKFQEKLVTNNGDVLDLDEDNEVDDVELQWFKKNINAQVLPNGYCRLPVVAGGC
                     PHANACLDCTHFCTSKQFLPQHEEQLERTEELLAIAKDKQWQRQVETNSRVKERLEQI
                     IGSLTG"
     gene            22055..22432
                     /locus_tag="IV334_001076"
     CDS             22055..22432
                     /locus_tag="IV334_001076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000361059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAP8943164.1"
                     /translation="MDKQVRNTTEIVRLAKQKSKKTREKVDKAISKFSIEGKVINFNS
                     IAKEANVSKSWLYKEHDIRQRIESLRERQITANVVSKPKKSSRSEEILIKTLKRRVME
                     LEKENKKLQNQIQKLYGDLYNKE"
     gene            22586..23368
                     /locus_tag="IV334_001077"
     CDS             22586..23368
                     /locus_tag="IV334_001077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000067268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside nucleotidyltransferase ANT(9)-Ia"
                     /protein_id="HAP8943165.1"
                     /translation="MSNLINGKIPNQAIQTLKIVKDLFGSSIVGVYLFGSAVNGGLRI
                     NSDVDVLVVVNHSLPQLTRKKLTERLMTISGKIGNTDSVRPLEVTVINRSEVVPWQYP
                     PKREFIYGEWLRGEFENGQIQEPSYDPDLAIVLAQARKNSISLFGPDSSSILVSVPLT
                     DIRRAIKDSLPELIEGIKGDERNVILTLARMWQTVTTGEITSKDVAAEWAIPLLPKEH
                     VTLLDIARKGYRGECDDKWEGLYSKVKALVKYMKNSIETSLN"
     gene            complement(23494..24225)
                     /gene="erm(A)"
                     /locus_tag="IV334_001078"
     CDS             complement(23494..24225)
                     /gene="erm(A)"
                     /locus_tag="IV334_001078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001072197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(A)"
                     /protein_id="HAP8943166.1"
                     /translation="MNQKNPKDTQNFITSKKHVKEILNHTNISKQDNVIEIGSGKGHF
                     TKELVKMSRSVTAIEIDGGLCQVTKEAVNPSENIKVIQTDILKFSFPKHINYKIYGNI
                     PYNISTDIVKRITFESQAKYSYLIVEKGFAKRLQNLQRALGLLLMVEMDIKMLKKVPP
                     LYFHPKPSVDSVLIVLERHQPLISKKDYKKYRSFVYKWVNREYRVLFTKNQFRQALKH
                     ANVTNINKLSKEQFLSIFNSYKLFH"
     gene            complement(24283..24370)
                     /locus_tag="IV334_001079"
                     /pseudo
     CDS             complement(24283..24370)
                     /locus_tag="IV334_001079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771940.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ErmCL family antibiotic resistance leader
                     peptide"
     gene            24734..25396
                     /locus_tag="IV334_001080"
     CDS             24734..25396
                     /locus_tag="IV334_001080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HAP8943167.1"
                     /translation="MNTIESQFDKVAEDYDFVNELLNDYSFFVSNMSPKKGRALDIGC
                     GSGLLVEKLASYYDEVVGIDISNQMLDLAKSKRQLTNTVYLNMNAEQLNFNEKFDFIV
                     SRTTFHHLDDIASVIQQMKELLNEEGRIVILDNVSEVETPPTYVYKLGAIQEFLPHCF
                     KFGIKNAIRIYNHNTSKSWLEHLASDKYLSEQNYYDLYEKLLPGCQFHKMGWAMGVVW
                     TK"
BASE COUNT         8718 a         4086 c         5259 g         7405 t
ORIGIN      
        1 aataaagctt atgcaaagaa aaagaaatcc aatcacttgg gtttcttttt cttttattat
       61 tatgatgaat caagctttct gtttttgtca cgtatgttgt aatctttacg aaagagtaga
      121 gttggctact cataatcaac catacttccc atcatcgcta aatacgaaat aaatcagcat
      181 tcctacacca atcaaagcac aagctgcacc aagaaaaggg atgacattcc caaagttatc
      241 tgtattcata agtatgccac cacccacaaa caaaaagccg ccagcaatca ggtttttcat
      301 tttattcatc tcctttctta cgttatagta tttccttctt tcaatgagta ttttgaaagc
      361 tatcttattg tatgatgttc ttctaggcct cttctttatt atacaggcca ttttcgttat
      421 ctcatagaaa tattcctttt tatctgaaaa ctattgtata ccgtatgaca tttttcttca
      481 tgaagttgta agtatattct cattcaaatg aaaatttcat catagaagag attgtatttc
      541 taaattctta agagtaaaat acgaagtata acttggattt ggaaggatga ttggatgaaa
      601 gctattacgc ctgatatgac aacaaaattt aacaaacagt ttcaagcaaa tatcaaacag
      661 caggcattgc agcgggcagt tgtgaagaac ggaattactg cttctgctga aaatgtttca
      721 gcacatgtgg agaatacacc ggtattttct atagatttgg ctacggggaa agtggctaat
      781 caaatgcaat cggggcgttg ctggatgttt gctgcactga acacattccg tcataagatg
      841 ctgaatcaat tccatttaaa agagtttgaa ttatcacaaa attacacttt tttctgggat
      901 aaatatgaaa aggcaaatta tttctatgaa aacatcattg caacaggaaa agaggcaatc
      961 gatagccgga aagtagcttt cttactcgct actcctcaac aagatggcgg ccaatgggat
     1021 atgatcgttt cattattcca aaaatatggc gttgtgccta aatcggtgat gccggaaagt
     1081 gcgaatagtt caaattctcg agatttgaat aactacttga acaaattact gcgtaaagat
     1141 gcagtagtgt tacggaaaat ggttgctcaa ggagaaacat tagacgctat tgaagaaaag
     1201 aaagaagaaa tgctagaaag catttataac ttcttagcta tctcattagg aacacctcca
     1261 aaagaattcg attttgagta ccgcgatgaa gaaaagaact atcatcttga ccgaggactg
     1321 acaccacaaa ttttttatga taaatacatc ggtgtaaaat tagatgatta tgttagcgtg
     1381 atcaatgcac caacaaaaga caaaccattc aatcgttctt atacagtaga gatgctagga
     1441 aatgtcgttg gcgggaaaga agtcaaatat ctgaatgttg atatggaaac tttcaaaaaa
     1501 ttagcgattg ctcagcttga agaaggagaa tctgtttggt ttggttgtga tgtaggtcaa
     1561 tcttctacta gaactagtgg catcatggcc ttagatgctt attcaatgga tgatctattt
     1621 gatacggatt ttacaatgac taaagctgaa cgtttagatt ttggagaaag tctgatgacc
     1681 catgcgatgg tattgaccgg tgttgactta gtagacgggc aatcaacaaa atggaaagtc
     1741 gaaaatagct ggggcgaaaa agttggaaag aatggcttct ttgttatgag tgatgcctgg
     1801 atggacgaat atacgtacca aatcgttgta agaaaagatt tattgacgaa agagcaatta
     1861 aatgcgttca atgaagaacc aattgttcta tctccgtggg atccaatggg tgctttagca
     1921 tagacgagta tttcaatgaa gagtatgtag taaagataaa aagagctggc ttaagccagc
     1981 tctttttatc tttaaagtcc attccctgtt ttttatggtc cctgtgctat aattctaaat
     2041 aaaggataag gattggaaat aatcctttta tcattaaaaa tatgatgacg aagtagaata
     2101 aaattgggag taggggggaa agtcatggat atccaaactg ttttatctga tctgaaatta
     2161 aatggtaaag tgattccagt tgttggtggc gatgttaatc agacctatcg tataaaaacc
     2221 gaacatcgtg cttatttttt aaaaatccat ccgaatgtaa aaaaaggatt ttttgaagca
     2281 gaagtagacg gattaaaaga actgtcagca ttcgtacgtg taccggatac gtatatgcta
     2341 ggtgaaacaa gtgaaggcgc ttatttgtta atggaatgga tcgagccggg aaaaggggat
     2401 caacgagatc ttgctgcagc tttagctaat ctccatcagc aaacagcgcc acaatttggt
     2461 tttcgtaaag ataactattt gggtacactt gttcagaaaa attcttttga agaagattgg
     2521 tggacttttt tctttaaaga tcggcttgaa tcccagattt ctttggcaga agaaacgaat
     2581 cgatggaatg tacagcgtca agaaaagtat ctgcgattta aagaacgtgt attgaaatcg
     2641 gttgaaccaa aaaagatcac tccacgcctc ttgcacggag acctgtggag tggtaatgtg
     2701 ttctttgacc agcaagggca tccagttttt gtcgacccag ctgtttctta tgggaatcgg
     2761 gaacaagata ttgcaatgag ccagttattc ggtggtttcc gaccggaatt tttagatgct
     2821 tatcaaacta tatttccatt agaaaaggga tggaaagacc gactgccgat ttaccagtta
     2881 tattatcttt tggcacattt gaatatgttt ggagaaagtt acggttccca agtagatcaa
     2941 ttactagaaa atttttaaga ggagggcgtg tttttggcaa aaaaatatac caaaaagcat
     3001 gaagtttcat attatgagtg tgacatcaac caaacgatga catttccttc gatgttagga
     3061 gtagttatca aaacgtctga agatcaaagc gaccagttgg gaagaggcag tgaattcgtc
     3121 aatagctttg gacttacttg ggtcatcacg aattattcca tggagattaa tcgtctccct
     3181 agagtcggtg aagtaatctc agtaacgact caagccatgg agtacaataa gtttttctgt
     3241 tatcggaact tctggatcca tgatgaagat gggaaagaat tagtcaaaat agaatctgtt
     3301 ttcgtattga tggattttgt gaatcgaaaa atgagcagtg ttaatgaaga gatcattgct
     3361 ccttttgaga gtgagaagat caagaaaatc aagcgtcagg aaaaaattga aaagattgat
     3421 tcgggcttca tgttgccata tcgtgttcgt ttttatgata tcgatagtaa tcagcatgtt
     3481 aataatgcga tgtattttaa ttggatcatt gacgtattag gatatgactt tttgactacg
     3541 catgttccag aaaaagtaat tattcgattt gataaagaag tagagtacgg gaatgagatc
     3601 gaaagccatt ttgaacaaat ccctatcgaa aatggtgtca aaacaaaaca tgaaatccga
     3661 atgcaagaac agctgtattg tgaagcaaat attatatgga aaaataaata aaaaagaggt
     3721 tgtgacagaa gggggcaact tcaagaaata agaagaaatt tacgaaaatt gtttctcaaa
     3781 tttttgtaaa ttttcgactt agttttcgaa gaagctattt ttgtcacagc ctctcttttt
     3841 aagtttagct attagataat gatgtttagt tttggataaa cgaaatcaat tcttcacccg
     3901 acaaaccata gtttacagca atcaatagtt ttaaacgtgc tttttgacta ttaatactgt
     3961 tacagaaaat aatccccatt ttttctagtt cttttccccc gccttcataa tcataaacag
     4021 gctctgcgat tccattgaaa caacgagaaa caagtactac agggattttt tggacaagaa
     4081 gcttttccaa agcagctaag gcttgtggag ggagattccc cgcacctaaa gcttcgatta
     4141 ccaaaccgtc tactttcgta tgggcgatgg cttccaataa gtccccttgc atcccagcat
     4201 atgctttaac gatcgggata gtcccatcta cagcggaaat gtccagtcgc tttgtttcta
     4261 aaagttcctg caagaataaa attcgattct tagttactaa gccgatggga cccaatgtag
     4321 gtgttcggaa agtagcaaca tttgtggtat gggtctttgt cacgtagcgt gcagaatgga
     4381 tctcatcgtt catgaccacc aaaaccccct tatctagcgc ttcttcacaa cttgcgacac
     4441 gaatcgcact ttcaaaatta taaaggccgt cacttccaag ttcattactt gaacgcattg
     4501 ccccagtcaa aacaattggt aatttgttgc caatcgttgt atcaagaaaa aaagcagttt
     4561 cttctaatgt atctgtaccg tgtgtgatca ccactccgga tatacctgat gcataagcgg
     4621 acaagatacg ttcttttaat accaacatgt gagaaggcat gatgtgtggt gagggaagat
     4681 tgaagatatc ttcaactaat aattctacat gcttggggat tgtgatttct gcatctaata
     4741 atgggtttgc cacatcagga ctaacaccgc ccgtgagcat gttttctttc atggcgatcg
     4801 ttccgccggt gtgtagtaca agtatttttt ttcattttac agacttcctt acgaataatt
     4861 ttgcatagcc gttaaatctt attgtatact atataaaaga gttttgctat cagtgaagaa
     4921 taacgaatag cctaacttat cataaaaaag ctgaccttat taaaatggaa aagactcaac
     4981 cttgacctcc taatacaatg gagtatctgg ttgagtttct tttacaagaa agcagccaag
     5041 gaaatcaatc acgaaggtgg ccggaaatga tcagaatcgg attaacatcg tttaaagaac
     5101 atgacaaatt aactggtaaa aagaataaca ctctttatga atatgcaggg tttctaccct
     5161 tagtggaatt agatactgcc tattatggaa ttccccgaaa gtcctcagtt gagaattgga
     5221 taagtgaagt acctgaatca tttcgctttg tagtcaaagt ctatcaagga atcagcggtc
     5281 aaggagactg gaagccttat tatcagaccg aagaagagat gattgcgcat tttttagatg
     5341 ttatgtcgcc aatggtagat gctggaaaga tgttttgctt tttagtccaa ttcccctcat
     5401 cattcaaatg tacgaaagaa aatgtccagt acttgcgcaa acttagaaag tggtttgagg
     5461 gttatcctgt ttctatagaa ttgcgggatt attcttggta cagcgatgaa tttcttgaac
     5521 gtacaagaaa ctttatgtct agagccgctt tttctttagc catcatcgat gagccacagt
     5581 tgctgaatac aacgattcct tttgatccat ttgtcaccaa tccgaacttt gctctttttc
     5641 gattccatgg taggaatgcg caaggatggc aagcaaatga taaagagtgg cgtaaaaaaa
     5701 gaacgttata tcgttattca gaaaaagaac tgcaagaact agcacatgaa gtcaaagtag
     5761 ttgattctaa gacaaaagag gtaggtgtga tttttaataa taattctggt ggtgatgcgg
     5821 ctgaaaatgc attagcactg caaaaaatat taaatatcaa gccagatgga ctcaatccaa
     5881 cccaaatgga tctgttttaa gggcttattt tattcaagaa aggggcgaag tctatgatca
     5941 aagctatttt ttttgatatt gacggaacat tggtcaccaa tcgttcaaaa gcgttggaat
     6001 caacaaaaca agcaattgaa tatgctcgga aaaacggcat tttatgcggt gtagcaacgg
     6061 ggcgcagccc agtaaagatc aaagaaatca ttgatgaatt ggaattagat atgtatgtcg
     6121 tgtataatgg tcaactggtt tttactgcag atcgcacgat tatcgaccac ccttttgaac
     6181 aaaaagtatt ggaacatatt gttgaatttg cagatgagaa ccatcgacaa atcgtttttg
     6241 gtgcgagaaa tcggttagat ggatcaacga cgatgctatt aggacaatcg atttttatca
     6301 agcgactagt cagcttttta ccgcgtaaat tcccggtacg tctgatgaaa aaaatcctcc
     6361 aggtttttag ccctcaccga caaaaggatc gttatgaaac gttagagata ttaaaagaac
     6421 caatttatca atgtatattg ctcagtccgg agtcagaaca gaaaaagtta gaacatcgct
     6481 ttcccgagtg tacgtttcag cgttcaaata cgtatactgt agatatcatc ccaaaaggtg
     6541 gttcaaaatt actaggcatc caagctttcg caaatgctgt cgaaattgaa atggaagaaa
     6601 ttatggcatt cggcgatcat tacaatgata tcgagatgct gaaaggtgta ggaattggag
     6661 tggccatggg gaatgctcag atcgaagtaa aacatgctgc agactttgtg actcaatcaa
     6721 atgaagaaga tgggatatac gaaggactga aacacttcgg tctgatttat tagtcaatga
     6781 aagaaggaaa aaatcagtat ggaagcagag acaccttttc aaaaagtaga aacattccac
     6841 aaaacttttg atccaagaag accagagata cccacggctt ttacagaaaa tgaagctcat
     6901 acacgtgctg cgttcaaaat agaagaactt gttgaatttg ttgcagctac ttcacctaat
     6961 aaagaacaac tgcatgaagc tgttatgctg ctgcacgaag cattagataa agcagaagaa
     7021 aaaatacttg gtaaaacaca gtggggaagt gatccgctgg ttgaacaagt agatgcactg
     7081 acagatatcc tttactttac ttatggttct ttttcattac taggaatagt gcctgatcga
     7141 atattcgaaa tcgtccatca ggcaaatatg gggaaattat ttccagatgg aaaacctcat
     7201 tatgaccctg taacaaataa agtgttgaaa cctgaaaact ggcagatgga atatgctcca
     7261 gaacctaaaa tcgcggcaga aatccaacgt cagattcaag aagcgattgc taagaagaaa
     7321 tccgctgata taggcatgtg cacaaatgaa aaagagtgat cagagtttca ctatttttgt
     7381 tatctttata cgatggaaaa acaaaagatt ttcgtttaag ttagtgaatg catgaaaata
     7441 gtatgctata atctagaata gatgtctttt gcacgagaga cgtattattt ggatttatgc
     7501 caagcagaac gccatctgtt gcataaaaga tgcatactga aaacggagga accataaatg
     7561 ggaaataatg ttatcatcgg aatcgtcatt gcaatcatta tagtcgccat tgtcgtcttt
     7621 ttggttagtt acttgatgaa gaaaaaaaac caagatcgat tggatgcctt agaaaaacgc
     7681 aaggaagaat tatttgactt gccagtgatt gaagaagtcg atgatgttaa aaaaatgcat
     7741 ctggttgggc aaagccaaaa tacctttcgt gaatggagtc aaaaatggaa tgacatatct
     7801 acttcctcgt tcgctgaact agaaagtcaa attttcgaag tggaaaacct caatgaaagt
     7861 tttcggttct taaaaggaaa aaaagcagtt gttgaagcag aagctacaat ggaccaaatg
     7921 gagaaacaag tgacggaaat ccgtgaaggt ctaaaggaat tgcgggaaac ggaagaacgc
     7981 aactcactag ctgttcagca ggcacttgac gtctatgaag aactgaaaga aaaagtcaga
     8041 gaagacgagc atgaatacgg accagctctt tttgaattgc aaaaacagat caaagtgatc
     8101 gaaaaagaat ttacgcagtt tgtgacttta aacacttctg gagaccctgt tgaagcccgc
     8161 gaggttttgg ataaagcaga aaaacatacg tatgaagtgg aagctctgat gaaaaaaatt
     8221 ccaccgctgt atgaggagct ccataccact ttccctgaac aacttgaaga aatcagtgat
     8281 acttacgaga aattgatgga agaacaatat gtattcccag aagaagatct tgctgaagat
     8341 atcgcaaaag taagtcgaag aattgaaaat tcattagcca atcttgaaaa aacagaagtt
     8401 gaaacggtcg aatttgaaaa ccgtgaaaca gctgatttga ttgattcgtt atatgatatc
     8461 ttagaacggg aaatggaagc tcagagatat gtaaaaacta accaatcaac gattgcagag
     8521 tatatcaaac atacaacaaa gaataaccgt caattattga ttgagctgga tcacacatct
     8581 caaagctata cattgaacca caatgaaatt ggccgtgtac gaggtttcca aaccgaagtg
     8641 gaagaaatgg aacgtcaaaa tgaacagatg attccacaga tccgtcaaca cgaaattcct
     8701 tattcagaag tgcgcacgtt ctataaaaca gtcttcaaag tcttagaaga tatcgaaaag
     8761 caacaagtag agatcgacga ctcattacat gaattgagaa aaggcgaaaa agaagcgcaa
     8821 gaaaagatcg atacattcga attcaaattg cgcagtttga aacgttttgt agaaaaacaa
     8881 cgtttgcctg gcttgccgaa tgattaccta gaattctttt ttgttgctac tgaccgtatt
     8941 gaagaactaa gttttgtctt gaataaaatt cgagtaaaca tggaagaggt caatcgttta
     9001 gttgctctat gtgaagaaga tctagaatta ttagataaga agacacatga tttagtagat
     9061 gcagcagcat taacggagca aatgctccaa tatgcaaatc gttatcgtca tacacacgaa
     9121 gaagtgagag aagcaattga taaaagctac tatttattca ataaagaata tcactatcaa
     9181 gaagcattag acgaaatcgg tacagcatta gagcgagtag aacctggtgc tttcaaacgc
     9241 atcgaagatt tttattttaa tcatccagac ttagtataaa gcaaaaaagt tagtaaacca
     9301 gcaagagcaa gacggattat tgtcttgttc ttgtcttttt ttgtatattt gaaaattgaa
     9361 acaagtgaaa atttcacctg ctcttgaaag caccttggac aaccgttata atagaaaaga
     9421 atttcaaaaa agaggggttt cttatgatat attttgataa cagtgcaaca accccaatct
     9481 ttccgcaaag cttagatgca tatgtaaaaa ccagtcagcg gattattggg aatccatcaa
     9541 gcttgcatga tttaggcaat caagctaacc ggttgttgca gcaagcaaga aagcagatcg
     9601 ctgatttgat ccatgtagaa ccagaagaaa tctattttac ttcaggcgga acagaaggag
     9661 ataactgggt attaaaagga acgatgatcg aaaaaagaga atatgggaat catatgatca
     9721 tttcaggagt cgaacatccg gccgtcagcg agacagcaga acaattaaaa gaattaggaa
     9781 tcgaagtaag tatcgcccca gtggataaac gcggatttgt acgtgtcgac gaattgaaag
     9841 aattaataag aaaagagaca gtattagtct ctgttatggc tgtgaataat gaagtaggat
     9901 cgattcagcc catccaggaa atcagtgatc ttttggatga ttttcctaaa attcatttcc
     9961 atgtagatgc tgttcaggcg attggaaaag taccattctc gcaatggttg acagaaagag
    10021 ttgattttgc aacattttca gctcataaat tccatggccc acggggaact ggtttcattt
    10081 attggaaaaa gggtcgtcgt ttagcaccat tactgacagg tggagggcaa gaaagaaatc
    10141 agcgaagtgg gacagaaaat gttccaggta ttgtggctat ggttaaagct ttacgtttat
    10201 cattagagat aaaggaacaa aaacctgagc agacacaacg cttaaaagta gctctaatca
    10261 atgcgttgaa gacgtatgag aaagtgacga ttttttcaga aggaactgac tatgcaccgc
    10321 atattttatg ttttgctcta aaaggaattc gcggcgaagt tctcgtccat gccttagaag
    10381 aaaaacaaat atttgtttct actactagtg cctgctcctc acgtaaacat atggcaagca
    10441 gtacattgca cgcaatgcat gtaccaaatg atctggctac atctgctgtt cgaatcagct
    10501 tagatgaaaa caattcgctt gcagaagtcg agcaatttat gattattttc aagcagcttt
    10561 ataagaaatt ttctgcaatc aattaaaaga ggagtgttct tgtgaaatac agtgaaatta
    10621 tggtccgtta tggggaactt tcgacaaaag gaaagaatcg aaaaagcttt atcatgcaac
    10681 tagcacaaaa tgtcagacaa gctttgaaag actttccaga aatcaaaatc catgctgatc
    10741 gggaccggat gcatctttta ttgaatggcg cagacgggca gctagttatt ccaaaactag
    10801 caaaaatatt tgggatccaa aatttctctc caagtattcg tgtagaaaaa gatgtcgaag
    10861 ttttgaaaca agctgttcaa gacatcatga aagaaattta tactgggaaa gaaacattta
    10921 aaattatcgc taaaagaagc gatcaccaat ttgaattgac aagtaatgaa ttgaatcaaa
    10981 cattaggtaa tgctgttttt gatattttcc cgcatatcca agttcaaatg cgtcaaccag
    11041 atattccatt acgtgtagag attcggcgtg atggcgctta tctatcgtat gaaacgatca
    11101 agggagctgg aggattgccc gtaggaacaa gcggaaaagg aatgcttatg ctttctgggg
    11161 ggattgattc tcctgtagca gggtactttg ccatgaagcg gggagtggaa attgaagctg
    11221 ttcattttgc tagtccgccg tatacaagtg aacaagcact gcaaaaagca aaagatctaa
    11281 cagccaaact agcaccttat gtgggaacga tccattttat cgaagtgcca tttaccgaga
    11341 ttcaagaaga aatcaaaaag tcagtgccac aaggatattg gatgacaatt actcgtcgaa
    11401 tgatgttgcg tttgaccgat gaaattcgcc gtattcgtca tggtttagtc attttgaatg
    11461 gtgaatcatt aggtcaagtg gcttcacaaa ctttgcaaag tatggtggct atcaatgaag
    11521 taacgaatac gccaatcatt cggcctgtag cgacaatgga taaacttgaa atcattgaag
    11581 tagcagaaca gatcgataca tttaatcttt ctatccagcc ttttgaagat tgctgtacga
    11641 tttttgctcc tccacagcca aaaacacgcc ctcaactgga aaaggtccat caatatgaag
    11701 agcgtttagc aatagatgaa atgatccaac gagcactagc tggcttgaag atggaagaga
    11761 tacaagcaga atcgtctaat gaaaaaactg aagaattttc agaattgcta taagaggtta
    11821 tgaaaatagt gttttgacca gagtattaga gaaacaaaac gaaaaaatag ctccatgtat
    11881 tttaccgttt ttgaactaat gtcgaagatt agctgattga ataccattta tcaagaagtt
    11941 atggaaaagc cggttctttt ttactaactg aaaaataaaa ttttatttag agatcaagct
    12001 acagttgata aatgatataa aaaagagaat ttcccatgaa atagcgaggt tgtgacagaa
    12061 gtgggcagct tcaagaaata agaaggaatt tacgaaaatt gcttttcaaa tttttgtgaa
    12121 atttcggctt atttttcgaa gaagttactt ctggaacacc gtgtatcaga ggttgcgaaa
    12181 aagctgccct gcatcaagta ataagaacag ccaaacaaaa atagttcctc atattttacg
    12241 tttggttgag cttattaccg cagatgcaag cttttgaaca ccgttttatt tgtctcacac
    12301 ccttttcctt cctacttgta accttgctcc cgtgttagaa acatgttaca ctaaatatgt
    12361 gaaattatta acaaggagcg tcgttatgcg agaaaaaaca gatacactgc caaaagcaac
    12421 tgcgaaaaga ttacctttat atttacgcta cttgaagatg ctggatgact caggtataag
    12481 tagaatcaaa tcaaatgagt tcagcgaaat cacccaaatt ccttctgcaa cgatccgcag
    12541 agacttctcg caattaggcg agctaggcag aagcggatat ggctatgatg tcccttttct
    12601 tatcgatgta ttcaataata tccttaatac taaagaagaa aaacgaatcg cattagtagg
    12661 atatggaaat cttggcaaag cattgaaaca caacaatttt agaagaaatg aaaatctaaa
    12721 tatcgtgtgc gtctttgata atgatccagc actgatcaat cgagtaattg atggagaaat
    12781 gatctatccg attgatcgtt tcgcggagat agccaaagca aaaaatgtga cagtagcaat
    12841 ctctacggta ccaagtaaat attcacaatc tgctattgat gaaattgtca aaggaaatgt
    12901 aaccgctata cttaattttg cgcctgacag agtaactgtc ccagcttatg tcaatgttca
    12961 atacattgat ttgacaaccg agttgcagac gcttatttac tttgatgaga attactcaga
    13021 agttttttct taggaaaatt tgagtaggct tattgatctt gcgagaactc gtcgtaattc
    13081 tttcaaaaat tttttagtac taaatgaagt ggttttttca cgaccacttg ttgactaaaa
    13141 aaaaaaacca ttgtagaatg aagcataacg ctgaacaaga ggagtaactg gcacattcat
    13201 tcttagagag agaactatct gctgaaaggt tctcaatgac aaccagtgaa ggtagcttgg
    13261 gagtttctgt cttgaatgga gtagagatag acgagtcgtg atcgttaact cacttgagag
    13321 gcaataatga ttgattattg caatttaggt ggtaccgcgt aaatcatacg tcctagaata
    13381 cgaaagtatt ttaggacgtt ttttagttta aaggaggaaa acagcatgac ggaagaaaat
    13441 aacctatcaa caaaatataa tccgcaagaa gttgaagctg gacgttatca gcattggtta
    13501 gaacaagatt tgtttaaacc gagcggagat aaaaaagcaa aaccttattc aatcgtaatc
    13561 ccgcccccaa atgttactgg aaaacttcat ttagggcatg cttgggatac gaccttgcaa
    13621 gatatgctga ttcgtcaaaa gagaatgcaa ggatatgata ctttatggct gcctggtatg
    13681 gaccatgcag gaatcgcgac acaagcaaaa gtagaagcga agcttagaga gcaagggatt
    13741 tctcgttatg atctaggtcg tgaaaagttc atcgatcaag tgtgggaatg gaaagaagag
    13801 tatgcgagcc acatccgcga acagtgggct aaaatgggct tatctttaga ttacagtagg
    13861 gaacgtttta cattagatga tggattatct gaagcagtac gtaaagtttt tgttacctta
    13921 tacgaaaaag gtttgattta tcgtggtgaa tatatcatca actgggatcc tcaagcacga
    13981 acagctctat ctgatatcga agttattcat aaagatatcg aaggcgcctt ttatcatatg
    14041 agctatgaac tagcagatgg atcaggcgta gtagaaattg cgacaacccg tccagaaacg
    14101 atgttgggag atacggcgat tgctgttcat ccagaagacg agcgttatca agcgctcatc
    14161 ggcaaaaaag tgattctacc tttagtcaat aaacaaatcc caatcattgc agatgaatat
    14221 gtagatatgg aattcgggac aggtgtagta aaaatcactc cagcacatga tccaaacgac
    14281 tttgaggtag gtaatcgtca tgatcttcca cgcgtaaatg ttatggatga tgatggtacg
    14341 atgaatgaac tagcaggaaa atatgctgga atggaacgtt ttgctgctcg taaagcaatc
    14401 gtgcaagatc tgaaagaaat cggtcgattg attaaaatcg atacgatgat ccacagtgtg
    14461 ggtcattccg agcggacagg tgtcgttgtt gaacctcggc tgtctactca atggtttgta
    14521 aaaatggcgc cgttagcgaa aaaagccatt gataaccaag aaacagatca agcagtggaa
    14581 ttcttccctc caagattcaa tcaaacgttt ttacgttgga tggaaaatgt ccatgattgg
    14641 gtaatctcac gacaattatg gtggggacat caaattcctg catggtatca caaagaaaca
    14701 ggtgaaatgt atgtagggat ggaagcacca gcggatagtg aaaactgggt acaagatcct
    14761 gatgttttag acacttggtt tagttcagct ttgtggcctt tctcaacaat gggctggcca
    14821 gatacggaaa gcgaagatta taagcgatac ttccctacta gcactttagt aaccggttat
    14881 gacatcattt tcttctgggt aagcagaatg attttccaaa gcttggaatt tactggcgaa
    14941 cgtccatttg agaatgtctt gattcatgga ttgattcgtg acgaacaagg gcggaagatg
    15001 agtaaatcat taggtaatgg aatcgatccg atggaagtaa tcgaaaagta tggagcagat
    15061 gctctccgct ggttcttatc gaatggttct gcaccaggac aagatgtccg cttcagctat
    15121 gaaaaaatgg atgcttcttg gaactttatc aacaaaatct ggaatgcttc acgtttcgtt
    15181 atcatgaata tcgaaggaat gaccgtagaa gagatcgatt tatctggaga aaaatcagtg
    15241 gctgaccggt ggatccttac tcgtttgaat gaaacgatcg aacgagtaac tgagctattt
    15301 gatcgttttg aatttggtga agcaggtcga caactgtata actttatctg ggatgatttt
    15361 tgtgactggt acatcgaaat gagtaaagaa gtactttatg gagatgatgc tgtttcaaaa
    15421 caaaccaccc gtagcatctt agtccataca cttgatcaga tcctacgttt attgcatcca
    15481 atcatgccat tcgttacaga agaaatatgg gaacatattc ctcatcaggg aaattctcta
    15541 gtagtggcag aatatcctgt tgtacgtcca gagtttgatg atgaaacagc ttctaaaggg
    15601 atggaagtat tgaaagaatt gattcgagcc gttcgaaata ttcgttcaga agtgaataca
    15661 ccattgtcta aaccaattac gttgctaatt aaaacaaacg atgctaaaat cgatcaattt
    15721 ttagttgaaa atacaagcta tatcgaacgc ttctgtaatc ccgaagagtt gacgatatca
    15781 agtgagatca ctgcgccaga tctagcgatg tcagctgttc tgacaggtgc agaaatcttc
    15841 ttgccgttag caggattgat caatattgaa gaagaaatca aacgattaga aaaagaactg
    15901 gaaaagtgga caagcgaagt aaaacgtgtc caaggaaaac tttctaatga acgttttgtt
    15961 tctaacgcgc cggaagaagt agtagaggca gagcgtgcaa aagaaaaaga ttatttagag
    16021 aagcaaacgg ctgtcaaaga acgaatcgaa agtttgcgta ctattcaata agaatagaaa
    16081 aagagagtgt gaaatcagct agctattact aaaagctagc tgattgaaca tcgttacagg
    16141 aaagagtttg agcctcactc ttagcttcga ttcataagga gatgtgacat aaattttgtc
    16201 gcatctcttt tttagtctac tcttattatg taggagaagg agggaggtgg atcatgtatc
    16261 aagagtcaat aaagtagttg ataaaatcgt tcctctctct gtttcttttc tctttgtcat
    16321 acttttattt ttcttatgtg ttctatttgt tataataaaa gagaaatggg ggaattgttg
    16381 atgacgattg aagaagcaat agaatggatc catagtcgat taccatttgg ttcacgtcct
    16441 ggccttgacc gagtcgaagc tttattagaa gtggtagggc atccagagag aaaagtaaaa
    16501 acgatccata tcgctggaac aaacggaaaa ggttctactg tagcttattt gagaagccta
    16561 ttagaagagg ctgggctgac agtaggtaca tttacttcac cttatatcga aacgttcaat
    16621 gaacggatcg caatcaatgg caaaagcatt ccagacaatg atttgatcca gcttgtaaaa
    16681 aaatatcagc cactgatcaa acaacttgat caatttgaag cagtagctgg tattactgaa
    16741 ttcgagacat taacagcaat ggcatttgat tattttttga tgaagaaagt ggacgtagcg
    16801 attatcgaag ttggcttagg cggcttattg gattctacca atgtagctgt accaatgctg
    16861 acgggaataa caacgattgg acttgatcat accgaaatac ttggagatac aattgaagaa
    16921 atcgctgcac aaaaagctgg aatcatcaaa caaggcgttc cagtagtaac aggaaatatt
    16981 gccccagaag cttttactgt catccaacaa acagctaaag caaaaaatgc accgctttat
    17041 gtatacgggg aaaattataa aataaactat ctgcatcctg atgagatgtg gggagaagta
    17101 tttgaatttt ttaatgaaat aggaaagatc cctcaattaa ccactccgct tttagggcaa
    17161 catcaagctg aaaatgcagg agtagccatc gaattgtatt ttgtttactg tcaattggaa
    17221 aagctgcctt tccgagaaaa agatgttttg aagggattga agaaatctcg atggccagga
    17281 agaatggagc gtttgagtaa cgagccatta gttgttttag atggtgccca taatacgcat
    17341 gcaatgaacc ggcttgttga gaatttgaaa aaagaattca aaggatatca aatcaacatt
    17401 ttgttttccg ccttacgaac taaaaatgtt agagaaatgc tagaacgatt gcagcaagta
    17461 ccaaatgtcc atatttattt gacgaccttt gaacatccaa aagccatcga attaagtgaa
    17521 gtatcagatt tagcaagtga gagagtatca atcgtttcat tgtggcaatt cggattagca
    17581 gatatattgg aaaagatgac aaatgaagac gtattagttg tgaccggttc actttatttc
    17641 atttcagaag ttcgccattt attaatacaa ttaggaggag atcatgaagt ataatgcagt
    17701 gatttttgac atggatggtt tgctgttcga cacagagtta gtgtattacg aagccacaca
    17761 agtagtagca gacaagatgg gttttcctta tgataaagac ctgtatttaa aattcttagg
    17821 tgtttcagat gaagaagtat gggcgaatta tcatatgatt ttctcggaat ttggcaaaac
    17881 aagtgtacaa agattcatag atgattcata ccaggaaacg cttgaacgtt tttcgcaagg
    17941 agcggtacaa atgaagcccg gtgtgttgga actcctctct tttttagaag aacatcagat
    18001 tcccaaagtt gtcgcttcca gtaaccaacg agcagtcatt gaacttttat tgaagaagaa
    18061 caatctgttt gaacgatttg agacgattgt ttctgcagaa aatgttcaac gagcaaaacc
    18121 agacccagaa attttcttgt tagctaatca gaagctaggt acagcgaaga aagaaacatt
    18181 gattttagaa gattcacaaa atggtatttt agcagcttct gcggctgaga tcccggtagt
    18241 catggtacca gatctgcttc caccatccca agaattgatc aaaaaaacag ctggggttgt
    18301 ttcttcttta catgaaatcc ctgcattgtt aaaataaaaa tccatctctt ttcgtatctt
    18361 attttagata agatagaaag gagatgtttt tttgggaaaa cgattgatca aagaagtacc
    18421 tactagctct ttaccaaggg aacgtatgga aatttatggc gcggaagctt tatcggatca
    18481 ggagcttttg gcaatcttac tgcgtaccgg tcaacaccct tatagtgtga tgtcgattgc
    18541 tggaaatctg ttaaaaacgt ttggaggact ggcctcttta cgacaggcta ctttgcatga
    18601 gttagaagag attcgaggga taggacgcgt gaaagcaatc gaaatcaaag ccttgatcga
    18661 attaggtcga cgaattcaga cggatcatga aagagcggct ccaccggttc gtaccagcta
    18721 cgaattggcc cagcaattga ttctggaaat gaaagatcac aaacaagaac atttggtttg
    18781 tatttatttg gacacgaaga atcaagtgat cttaaaaaaa acagtgttca tcggttcgct
    18841 gaatcagtca gtcgctcatc ctagagaaat tttccattat gctgtacgtt attgtgcagc
    18901 aaggatcgtt ttagcgcata atcatccttc tggtaatgtt ttaaccatag tataataaat
    18961 aatagtagtt aaagtagttt aagaggagat attaaaggga tattttactt taaataaaaa
    19021 ttaacaaggt ttataatttg tagtagttaa agtatttaaa gaggtgggaa catgtgaagg
    19081 ttcaaaggat agaagtggag aataagccgt atccattgta tttattacta gataaagaat
    19141 accagctaat agaaccagta atgaaattta ttaaatactt agataatact ggtaagtctc
    19201 ctaataccat taaagcatac tgctatcatt taaagttgct gtacgagttc atggaacaga
    19261 gaggtgttat tcttaatgat attaactttg agttgttagc agacttcgta ggttggttga
    19321 gatatccttc agcatcaaat gtaattgatc ttcagtcaaa aaaagccata agagaagaaa
    19381 cgacagtgaa tacaatttta aatgtagtta tgagttttct tgattattta agtagattag
    19441 gagaatttaa atcaattgat gtatttaaac aagcaaaggg aagaaatttc aaaggatttt
    19501 tacatcatgt taataagggt agataccaaa agaatgtctt aaagttaagg gttaaaaaga
    19561 aacagataag aacattgaga tcagaggaag ttaagcaaat tattgatgct tgtcatacga
    19621 aaagagataa attaatttta atgcttatgt atgagggtgg tttaagaatc ggtgaagtgt
    19681 tatcgcttag gcttgaagat attgtcactt gggacaatca aatccattta acacctagag
    19741 atgttaatgt taatgaagct tatattaaat taaggaagga aagaacaata catgtgagta
    19801 aagaacttat gtcactttat acagattact tgatatatga gtatagtgag gaattggagc
    19861 atgattatgt ttttatttcc ttaaaagaag gctattttgg gaaaccacta aagtaccaaa
    19921 gtgttcttga tctagttaga agaatagtta aaaggactgg aatagaattt acatcacata
    19981 tgcttcgcca cactcacgca acgcagctaa ttagggaagg atgggatgtt gcgttcgttc
    20041 aaaagagatt aggtcacgca catgttcaga caacgttaaa tacctatgtt catctttcag
    20101 atcaggatat gaaaaatgag tttaataaat acctcgagag aaaggagcat aagaaatgaa
    20161 tgcttctagt aaaaggaaaa ttattagtca gagtgagatt agcaaaaaaa tagctgtaat
    20221 gaatgaagaa atgcaggggt tttgggctaa taatagttgg gatataagaa aatgtccaca
    20281 tccttctgcc atagaattaa gtaagaatcc tgctttaagg aatcgttggg ttcgttttga
    20341 acgtgttaaa aatctgtggt taagaacaga attgaaatat ttttattttt accatttaaa
    20401 caatggaata tggaatgcaa aaactgtctg gattagaaaa ggaacagtaa ttaataaaat
    20461 gttagatttc ttagatttaa agtatcctag cattacttca attactgaag ttcctattga
    20521 aaaagcaatg acggagtata gaacttattt gacaaaacgg ggtgttagaa ttaccactac
    20581 taattataag attactgcta atcaagaaaa aacacctgta aaagctaatt cctactatgt
    20641 tactaatcta aaacaattta tggagtttta tgagaacttt tattttgatg gagaggagtg
    20701 ggataaagac gtttgggata gacgtaactt acctttgcca gatgataagg ttaacccaac
    20761 acaatatgaa tatacaatta actttaaagg gtttcggaat acatatttta aacaacttgt
    20821 aaaaagatat tgtaagttga gattgaacat ggatagcttt tcctatgtaa gtgatattgc
    20881 ccaaagactt aaagagttct ttaattttct ggacatgaaa tttaaacaag ttcagagagt
    20941 acaccaatta acgagagtgg aaattgaagc atatttaagt gaactaaaca tgatgggaat
    21001 aaaacctagt acaataactg ggaggatctc tatattggaa ggactattta gtacccttct
    21061 taggctagaa tgggatgatg ttccttccaa aatattaatt tattctgagg actatccgaa
    21121 aataccaaga gcaaaaccac gctttataga tgaattcgtc ctagagcaat tgaacagtca
    21181 tcttgataaa ttacccgaat atatagctac gatgactatg attgttcaag aatgtggaat
    21241 gaggataagt gaattgtgca ccttgaaaaa aggctgtcta ttagaggaca aagatggaga
    21301 tttcttttta aagtattatc aatggaaaat gaaaaaggag catatagttc caatatctaa
    21361 agaggtagct ttacttatta aagttcggga agataaagtt tcagaggaat ttccagatag
    21421 tgaatacctc tttccaagaa aagatggatc gccattaaaa caagaaacat ttagaggtga
    21481 gttaaataaa ttagcttatg agcaaaatat agtggataaa tcaggtgaga tttatagatt
    21541 ccatgcccat gcctttcgcc atacagtagg aacaagaatg attaacaacg ggatgcccca
    21601 gcatattgtg cagaaatttt tggggcatga aagcccagaa atgacaagca gatacgctca
    21661 tatctttgat gaaactctaa aaaatgaatt tactaaattt caggaaaaac tggttaccaa
    21721 taatggagat gtgcttgatc tagatgaaga taatgaagtc gatgatgtag agcttcaatg
    21781 gttcaagaaa aatataaatg cacaagtgct tccaaatggt tattgtagat tgccagtagt
    21841 agcaggtggt tgtccacatg cgaatgcatg cttagattgc actcacttct gtaccagtaa
    21901 gcaattctta ccacagcacg aagaacagtt agagcgtaca gaagagttat tagccatagc
    21961 taaggataaa caatggcaaa gacaagtaga gactaatagc cgtgttaaag agcgtttaga
    22021 acaaatcatt ggaagtttga cggggtaatt atcaatggat aaacaagtta gaaatacaac
    22081 agaaattgta cgtttggcga agcagaaatc aaaaaagaca agggaaaaag tagacaaagc
    22141 gatttctaaa ttttcgattg aaggtaaagt tattaatttt aattcaatag caaaggaagc
    22201 taatgtttct aaatcatggc tttataagga acacgatatt aggcaaagaa tcgaatccct
    22261 tcgtgagcgt caaataacag caaatgtagt ctcaaaaccc aagaaaagtt ctcgttcgga
    22321 ggaaatcctt attaaaacct taaaaagaag agtaatggaa ttagaaaaag aaaataaaaa
    22381 attacagaac caaattcaaa aattatatgg agatctgtat aataaagaat aattattaat
    22441 ctgtagacaa attgtgaaag gatgtactta aacgctaacg gtcagcttta ttgaacagtc
    22501 atttaggtat atgtccaatc tagggtaagt aaattgagta tcaatataaa ctttatatga
    22561 acataatcag ttacgaggtg aaatcatgag caatttgatt aacggaaaaa taccaaatca
    22621 agcgattcaa acattaaaaa tcgtaaaaga tttatttgga agttcaatag ttggagtata
    22681 tctatttggt tcagcagtaa atggtggttt acgcattaac agcgatgtag atgttctagt
    22741 cgtcgtgaat catagtttac ctcaattaac tcgaaaaaaa ctaacagaaa gactaatgac
    22801 tatatcagga aagattggaa atacggattc tgttagacca cttgaagtta cggttataaa
    22861 taggagtgaa gttgtccctt ggcaatatcc tccaaaaaga gaatttatat acggtgagtg
    22921 gctcaggggt gaatttgaga atggacaaat tcaggaacca agctatgatc ctgatttggc
    22981 tattgtttta gcacaagcaa gaaagaatag tatttctcta tttggtcctg attcttcaag
    23041 tatacttgtc tccgtacctt tgacagatat tcgaagagca attaaggatt ctttgccaga
    23101 actaattgag gggataaaag gtgatgagcg taatgtaatt ttaaccctag ctcgaatgtg
    23161 gcaaacagtg actactggtg aaattacctc gaaagatgtc gctgcagaat gggctatacc
    23221 tcttttacct aaagagcatg taactttact ggatatagct agaaaaggct atcggggaga
    23281 gtgtgatgat aagtgggaag gactatattc aaaggtgaaa gcactcgtta agtatatgaa
    23341 aaattctata gaaacttctc tcaattaggc taattttatt gcaataacag gtgcttactt
    23401 ttaaaactac tgatttattg ataaatattg aacaattttt gggaagaata aagcgtcctc
    23461 ttgtgaaatt agagaacgct ttattacttt aatttagtga aacaatttgt aactattgaa
    23521 aatagaaaga aattgttcct tcgatagttt attaatatta gtgacatttg catgcttcaa
    23581 agcctgtcgg aattggtttt tagtgaaaag aacacgatat tcacggttta cccacttata
    23641 aacaaaagat cgatactttt tgtagtcctt ctttgaaatc aatggttgat gtcgttcaag
    23701 aacaatcaat acagagtcta cacttggctt aggatgaaaa tatagtggtg gtactttttt
    23761 gagcattttt atatccatct ccaccattaa tagtaaaccc aaagctcgtt gcagattttg
    23821 caatcttttc gcaaatccct tctcaacgat aagatagcta tatttagcct gactttcaaa
    23881 ggtaattctt ttgacaatat ccgtactgat gttataagga atattaccat atatcttata
    23941 gtttatatgt tttgggaagg aaaattttag aatatccgtt tgaatcactt ttatattctc
    24001 agaggggttt accgcttctt tagtcacttg acataagcct ccatcaattt ctatagcagt
    24061 aactgatcga ctcattttga ctagctcttt ggtaaaatgt ccttttcctg atccgatttc
    24121 tattacgttg tcttgtttac tgatattcgt gtgattcaat atttctttta catgcttttt
    24181 agaagtaata aaattttgcg tgtctttagg gtttttctgg ttcattataa ccttctcctt
    24241 actggttata atgaactgac attaagcgtt cattataacc aattaatttt gatttggttg
    24301 ataacgaact ttattaataa caaatataga aaaagtaccc atacttttat ctcctttcca
    24361 tttttttcat tatgaatgag ataaagtaat ttctactact gcgatactgg tgcacataat
    24421 tgaaacctcc ttatgtaact gaatatgatt gtatcatctt ttcattttag aataaacctt
    24481 tatatgtaat tttgcacttg acacttcaga tggacgtttt cttcaaaata atatgttttg
    24541 aaacttgata agagaagcca ctgtctttac taaaaagtaa aggctgcgat aaaggacagt
    24601 gactttaata aagttgcact gttgtattgt ctttggataa aattgtctaa agggaagttt
    24661 tatccccaac gaaaaaacat ctaaagtggt aagtaatgaa agaatgtcga taaagtagga
    24721 atgggggatc tttttgaata caatagaatc acagtttgat aaggttgcag aagattacga
    24781 tttcgtgaat gagcttttga atgattattc attctttgtg tctaatatgt ctccaaagaa
    24841 aggcagagca ttagatatcg gatgtggctc gggtttgtta gtggagaaat tagcaagtta
    24901 ttatgatgaa gtggtaggga ttgatatttc taatcaaatg ctcgatcttg ccaaatctaa
    24961 acgtcaacta acaaatacgg tctatctgaa tatgaatgca gaacaactta attttaatga
    25021 gaagtttgat tttattgtaa gccgaacaac ctttcatcat ttggatgata tagccagcgt
    25081 aatacagcaa atgaaggaac tgttgaatga ggaaggaaga atagttattc ttgataatgt
    25141 atctgaagtc gagacaccgc ctacctatgt ttataaatta ggggcaatcc aagagttttt
    25201 accgcattgt ttcaaatttg gaataaaaaa tgcgattaga atttataatc acaacacatc
    25261 taaatcatgg cttgagcatt tagcttcaga taagtaccta tctgaacaaa attattacga
    25321 tttatatgaa aagttgttgc ctggatgcca attccataaa atgggttggg caatgggggt
    25381 tgtctggaca aaataactta aagctgtggc tttgaaaagt tgaatttgtg attgttttca
    25441 gagctatcgg gtgctttagt ataaataa
//
LOCUS       DACDKM010000026        24484 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20834_2016
            SAMN12640370-rid9891663.denovo.026, whole genome shotgun sequence.
ACCESSION   DACDKM010000026 DACDKM010000000
VERSION     DACDKM010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640370
            Sequence Read Archive: SRR10040891
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 24484)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24484)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742835.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742835.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:04:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,012
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 29 of 75
            Pseudo Genes (incomplete)         :: 33 of 75
            Pseudo Genes (internal stop)      :: 19 of 75
            Pseudo Genes (multiple problems)  :: 5 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24484
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640370-rid9891663.denovo.026"
                     /isolate="E20834_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(2..850)
                     /locus_tag="IV334_001081"
     CDS             complement(2..850)
                     /locus_tag="IV334_001081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HAP8943168.1"
                     /translation="MSVISRIESMMEDYSSAEKKLANYIIGNIEKVPTMTANELAEAS
                     GLSAPTVVRFSKKIGFQSLTDFKITISTELQTGIDEGFSDIEANESFYSIKNKLGNNA
                     QVAIKETVDILEEETIQKVVECLESAETVFLYGVGASSLVVEDILQKWSRVGKPIIFE
                     KDIHVLLPQLVSNEKKKVLWLVSNSGRSADVVALAELAKSMNIEIIALTQFGNNPLSK
                     IADVLVQTSRPKEITNRSAATNSLLAQFATIDIIFYFYMAKNEKLSEKVKKTREAIQE
                     YFMDSK"
     gene            complement(1074..3014)
                     /gene="thrS"
                     /locus_tag="IV334_001082"
     CDS             complement(1074..3014)
                     /gene="thrS"
                     /locus_tag="IV334_001082"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HAP8943169.1"
                     /translation="MIKITFPDGAVKEFESGTTTLAIAESISKSLAKKALAGKVNGKL
                     VDLTRPIEEDASIEIITPDHEDALGLVRHSAAHLMAQAMRRLYPNIHFGVGPAIDSGF
                     YYDTDNGQNQVTAEDLPAIEAEMMKIVKENLPIERRVLSKQEALEIFASDPYKVELIS
                     ELPEEEVITAYQQGEFIDLCRGPHVPSTGRIQVFKLLSVAGAYWRGNSNNQMMQRVYG
                     TAFFDKKALKEYIRLREEAKERDHRKLGKELDLFMVSPEVGSGLPFWLPKGATIRRTI
                     ERYIVDKEVSLGYQHVYTPIMGDVELYKTSGHWDHYQEDMFPPMDMGDGEMLVLRPMN
                     CPHHMMVYKNTIHSYRELPIRIAELGMMHRYEKSGALSGLQRVREMTLNDGHTFVRPD
                     QIKDEFKRTLELMVAVYADFNITDYRFRLSYRDPNNTDKYFDDDAMWEKAQAMLKAAM
                     DELELDYFEAEGEAAFYGPKLDVQVKTALGTEETLSTIQLDFLLPERFDLTYVGEDGE
                     NTHRPVVIHRGIVSTMERFVAYLTEVYKGAFPTWLAPIQATIIPVSVDAHGDYAYEIK
                     ERLQMKGLRVEVDDRNEKMGYKIRASQTQKIPYQLVVGDKELEDATVNVRRYGSKETA
                     VEDLNIFIDAMEAEVKNYSREN"
     gene            complement(3351..3530)
                     /locus_tag="IV334_001083"
     CDS             complement(3351..3530)
                     /locus_tag="IV334_001083"
                     /EC_number="5.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-oxalocrotonate tautomerase"
                     /protein_id="HAP8943170.1"
                     /translation="MPFVHVELVEGRSPEQLENMMKDITEAVHKNTQAPKEHIHVIIN
                     EMKKGTYGVNGEWKK"
     gene            complement(3577..5400)
                     /gene="cadA"
                     /locus_tag="IV334_001084"
     CDS             complement(3577..5400)
                     /gene="cadA"
                     /locus_tag="IV334_001084"
                     /EC_number="7.2.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cadmium-translocating P-type ATPase"
                     /protein_id="HAP8943171.1"
                     /translation="MTHFKKFLLTLLIGGIALLSEFVFHQPALAFAIVAFTGGILAFL
                     MFLEMIKTLRSGRYGVDVLAITAILATLAVKEYWASLMILIMLTGGDSLEDYANKRAG
                     KELQSLLDNTPRIAHRFKEGKLQDIVIDEINIDDQLLVKPGELVPADGRVTKGESSVD
                     ESSLTGESRPETKKPGDQIMSGSLNGDGSIQMIAEKRAADSQYQMIIKLVEESKEKPA
                     RFVRLADRYAVPFTLIAYLIGGISWWFSKDPLRFAEVLVVASPCPLILAAPIALVAGM
                     SRSSKNGIVVKTGTTIEKLARTKTIAFDKTGTLTKGILEVEAIQPVNGIKKEELLLYA
                     ASAEQESSHILARSLVNNVKNSQLYPVSQLKEVTGQGIQAVVNNHEVKIGRASFVSAE
                     EIETSNTAIYVTIDRSFAGTILFSDQIRPEAAKTIKELRKLDVKNLMMITGDGSAIAQ
                     TIAEKVQLDSVHARSLPQDKLTILESIPKDQRPVAMVGDGVNDAPALAAADIGIAMGA
                     HGSTAASESADAVILKDDLLRVAKAVVISRDTMRIAKQSVLIGIFICIFLMLIASTGL
                     IPALFGAVLQEVVDTVSILSALRAKKDPAIFPDSVPKTNIV"
     gene            5678..7957
                     /locus_tag="IV334_001085"
     CDS             5678..7957
                     /locus_tag="IV334_001085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAP8943172.1"
                     /translation="MNEKQLEQTHVDETIELIQIEQNMLDTKQQELTQEMNHSTKDTA
                     NHKIRAGSNEAFYESAVEYRQHEQELLLKYHTLEAQQKRLQTLSVMKDSPYFARIDFK
                     EDRETETLYLGIASLRDLAEETIVIDWRAPIANLYYEGEIGPAFYETETDRIDVDLQL
                     KRQFKIQEGRILSMVDTSEVINDDFLLEILDESSSNHMKNIVSTIQKAQNAIIRDTTS
                     KVMLIEGIAGSGKTSALLQRIAFLLYHNRKWLEAENVLLFSPNHLFSDYISTVLPSLG
                     ESGVPTQTFKNYLVQLLPEFSLLDEQQQESGFLLGEKDPIQVMKSGLTLVDQIDRYIQ
                     GITNYGPLFRDMKINGRTILSKESIRQWYKETNEQLPLHHRLSLLQTKLLKKLGGLQK
                     DETRQQWVKDLAEEQLQELYATDPNLEYTEQKERSLRKKLANQIVRKRFRRIHRGIMN
                     YQFVNITKQYLHFLQTVPLSLLTRHKISETRWQEHLTTVRTHLRQRELRQEDAVLFFL
                     LMRRTYPVTVTHKARYIFIDEMQDFAPSQVALLQALFPKANLTFCGDLNQNVFGNETI
                     VGSLEDLFVDREVTRFQLTTSYRSTKEITDFANHFIVKEHQVETTARKGDLPLLIQGT
                     TDDIKLNWLADTIRTTQGKARYWRTAIIAKTQEECEALYAQLPDELKQQVQLILNESD
                     FMKRSIILLPAFLAKGLEFDRVFLWDVDAEHFSSQQDKLILYTMCTRAMHELTLFTSN
                     NESPLLNNMDISSYEKKVL"
     gene            complement(8050..8697)
                     /locus_tag="IV334_001086"
     CDS             complement(8050..8697)
                     /locus_tag="IV334_001086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8943173.1"
                     /translation="MEAKITLEPFERILSGYRKVEELAVNVTDCSKLAQKYARFGVEG
                     YRLGNYVGTGYLNRYLECMVDRAPMLIYRQNYLIPLLFRRSDSAFRLFEEEYRMEAFF
                     LLLEWSLKHRPEKILIERNEKIDTKKNKVIDSAYLAFRVSEILDCGGYPISNFQSIDQ
                     FIEWNRIYRLIDNGGIGRHSKVFDPEYPENMEELKMIISLVKLKYPETDLDLYIE"
     gene            complement(8876..9595)
                     /locus_tag="IV334_001087"
     CDS             complement(8876..9595)
                     /locus_tag="IV334_001087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HAP8943174.1"
                     /translation="MGRKWANIVAKKTAKDANNSRIYAKFGIEIYAAAKSGDPDPHAN
                     QKLRFVIDRAKTYNVPKHIIDRAIEKAKGAGDETYSELRYEGFGPSGSMVIVDTLTNN
                     VNRTAADVRAAFGKNGGNMGVSGAVSYMFDNTAIFAFAGEDADEILEYLMEKDIDVRD
                     VMEEEGQIIVYGEVADFHSIQEALKEKGITDFSIAEIQMIPQNEVTLSPEDQETFEKM
                     IDALEDLEDVQHVFHNVALED"
     gene            complement(9726..10877)
                     /locus_tag="IV334_001088"
     CDS             complement(9726..10877)
                     /locus_tag="IV334_001088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase"
                     /protein_id="HAP8943175.1"
                     /translation="MKSCGIIVEYNPFHNGHRYHVEMARKITGAEVVIAVMSGNFLQR
                     GEPAIIDKWHRANEALQNGVDLVVELPVEWAVQSADYFAKGAVKILQTLDCDSLCFGT
                     EEETSFDYSSFGRFVQENQDRIEEAFQELSDQTYSYPQKMTEVFRSLYPDITLDFSSP
                     NHILGLSYAKENAKYVHPMELHPIRRKSAGYHEKIITDQRIASATAIRHALEEGKRIA
                     GLVPPQTEEDLSTCSLQTWESYWPYLRYRLLSSTLLELTDIYQMTEGIESRMKTAAAT
                     ADSFEDFVSKVKTKRYTWTRIQRLSCYVLLNIKKQEIPVQQNKNYLRLLGFTDAGRRF
                     LKEKKEISIYSRIGKKEAQEAELLVRSDQIYQLGGNIPEQNFGQIPIRV"
     gene            complement(11094..11834)
                     /locus_tag="IV334_001089"
     CDS             complement(11094..11834)
                     /locus_tag="IV334_001089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAP8943176.1"
                     /translation="MAYETFAFIYDEVMDETLYQQWLAFSKRHLQEDTKNILELACGT
                     GALAVAFAKDGYKVTGLDLSEEMLMIASQRAYEEDASIQFVEGNMLDLSEIGQYQAIT
                     CFSDSLCYMKNRQEVQQVFDEVYQALEENGRFLFDVHSTYQVDEVFPEYSYHYQGDNF
                     AFLWDSYPGSEKHSIEHFLTFFVEEQEGSGRFIREDELHQERTYPLESYLRMLENSGF
                     SKVEAFSDFTDEAASEKSKRWFFVAYKD"
     gene            complement(11838..12161)
                     /gene="rsfS"
                     /locus_tag="IV334_001090"
     CDS             complement(11838..12161)
                     /gene="rsfS"
                     /locus_tag="IV334_001090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HAP8943177.1"
                     /translation="MEAADSKRAEDIIALDVRNVSLLADYFMICSANSERQINAITEE
                     IIDKEEENKYEVKRIEGKEGGKWVLIDLGDVIVHVFHAPERNFYNLEKLWSDAPLVDL
                     SEWLD"
     gene            complement(12218..12811)
                     /locus_tag="IV334_001091"
     CDS             complement(12218..12811)
                     /locus_tag="IV334_001091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HAP8943178.1"
                     /translation="MNMDYSNEYTAYSRENLMQKIQMRMSEQRFKHVLGVEETAVALA
                     KKYGASPEKASIAALTHDYAKERPDEEFKMVIVRDGFDPELLNYNNAIWHGLVGASFV
                     ERELGITDAEILHAIRVHTTGAAKMSLLDKIIYVADYIEPGRDFPGVQDARAIAWADL
                     DEAVAFETKHTLAHLLAQEQQIYPKTIETYNYWVAKK"
     gene            complement(12795..13442)
                     /locus_tag="IV334_001092"
     CDS             complement(12795..13442)
                     /locus_tag="IV334_001092"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HAP8943179.1"
                     /translation="MNTQAKTFVRSQLFPEEMPQFLEKKKQVGILGGTFNPVHLAHLV
                     MAEQAGRNLGLDRVFLMPSYQPPHVDEKQTIDAKHRLNMLELAVEDNPFLQIETIELA
                     RGGKSYTYDTMKELTQNNPDTDYYFIIGGDMVEYLPKWYKIDELTSMVNFVGIRRPGY
                     TTDTPYPVIWVDVPEIDISSTKIRQKIKEGCSIRYLVPDKVIDYIQNEGLYEYGL"
     gene            complement(13455..13766)
                     /gene="yhbY"
                     /locus_tag="IV334_001093"
     CDS             complement(13455..13766)
                     /gene="yhbY"
                     /locus_tag="IV334_001093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HAP8943180.1"
                     /translation="MSLRGKQKRFLRSKAHHLQPIFQIGKGGINSAMIVQIEEALEKR
                     ELIKVSLLQNTDEIAEEAAQVLEKEIDCEIVQIIGRVIVLYKPSTKEKYQRLSKEVKA
                     I"
     gene            complement(13782..14891)
                     /gene="yqeH"
                     /locus_tag="IV334_001094"
     CDS             complement(13782..14891)
                     /gene="yqeH"
                     /locus_tag="IV334_001094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YqeH"
                     /protein_id="HAP8943181.1"
                     /translation="MTEELQCIGCGAVIQTNKPGELGYTPQTALEKGLASGEVYCQRC
                     FRLRHYNETQDVSLTDDDFLRLLNELGQKDALIVNVVDIFDFNGSLIPGLHRFVGDNP
                     VLLVGNKVDILPKSLKKTKLTQWMKERAYEAGIRPVDVLLTSAKKSHEMTELLEKIEE
                     YRDGRDVYIVGVTNVGKSTLINQIIKNTAGVQDVITTSQFPGTTLDKIEIPLDDGHFL
                     IDTPGIIHRHQMAHYLGKKDLRIIAPLKEIKPKVYQLNEGQTLFLGGLARFDFISGNR
                     ASFIAYVSNDVNIHRTKLEKADEFYQKHVGGLLQPPRPDEVEAFPELVRFEFSVKEKT
                     DIVFAGLGWITVTAPVVIAGWAPKGVDVLKRKALI"
     gene            complement(14888..15415)
                     /locus_tag="IV334_001095"
     CDS             complement(14888..15415)
                     /locus_tag="IV334_001095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqeG family HAD IIIA-type phosphatase"
                     /protein_id="HAP8943182.1"
                     /translation="MFSNYKPTWMVEAIYQITPAQLKNLGIKAVLTDLDNTLIAWNNP
                     DGTEELLTWILEMKNAGIPVVVVSNNNSKRVARAIEKFDLLYVARAMKPLARGINIAK
                     KQLDLADDEIVMVGDQIMTDIRGANRAGIRSILVKPIIATDSWKTQFNRFWEKRIMAY
                     LLAKHPDMGWRGGIE"
     gene            complement(15595..16380)
                     /locus_tag="IV334_001096"
     CDS             complement(15595..16380)
                     /locus_tag="IV334_001096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyl transferase
                     subunit alpha"
                     /protein_id="HAP8943183.1"
                     /translation="MDKTAHDVVQLARAKERWTSLDFFEEIFDSFEECHGDRLFADDE
                     AIVGGIARLDGKPVTVIGIQKGRDLQENIRRNFGSPHPEGYRKALRLMKQAEKFNRPV
                     ITLINTAGAYCGVGAEERGEGEAIARNLLEMSDLKVPIIAIIIGEGGSGGALALGLAD
                     EVWMLEETIYAVLSPEGFASILWKDGSRAAEAAELMKITAPELRELGIVDRVIPETFN
                     GEQLPRYKVAHIMKKALIERLTELVRLDQHKLLEKRYKRFRQY"
     gene            complement(16373..17242)
                     /locus_tag="IV334_001097"
     CDS             complement(16373..17242)
                     /locus_tag="IV334_001097"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit beta"
                     /protein_id="HAP8943184.1"
                     /translation="MALFKKKKYIRINPNRESKPANSPSVPDNMWAKCPNCKHILYTK
                     DIGEEKVCPHCGYAFRIGAWQRLALIIDEKSFEEWDTDLVTKDPLAFPEYTEKIKKMQ
                     DKTGLHEAVLTGKATIQGIPFIIGVMDPNFIMGSMGTIVGEKITRLFERATKESLPVV
                     LFTASGGARMQEGIFSLMQMAKISAAVKRHSNEGLFYLTVLTDPTTGGVTASFAMEGD
                     IILAEPQSLIGFAGRRVIEQTIKQELPEDFQKAEFLLSHGFVDQIVPRMELKQKIHTL
                     LELHTQKGWIDRG"
     gene            complement(17243..18622)
                     /locus_tag="IV334_001098"
     CDS             complement(17243..18622)
                     /locus_tag="IV334_001098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HAP8943185.1"
                     /translation="MFSKVLIANRGEIAVRIIRACRELGIQTVAIYSEADAEALHTQL
                     ADEAICIGPAKAVDSYLNVQEVLSAAIVTKAEAIHPGFGFLSENSRFASMCEECNITF
                     IGPKSATIDAMGNKINARQLMQEANVPVIPGSTGVLSTVEEALEIADRIGYPVMLKAA
                     AGGGGKGIRKVQSKEELPQHFSSAQQEAAAAFGNDDMYLEKIIYPARHIEVQILGDRF
                     GHVIHLGERDCSLQRNNQKVLEESPSVVISQTKREALGDAAVRAAKAVNYENAGTIEF
                     LMDEEGDFYFMEMNTRIQVEHPVTEMVTGIDLVKKQVEIAAGEPLNVCQEEVVFQGHA
                     IECRINAENPAFHFAPSPGKIQNLLLPAGGMGLRVDSAVYPGYTIPPYYDSMIAKIIV
                     HGNTRFEALMKMQRALSEFITEGIITNAEFQMDLISHPAVIAGDYSTAFLQEEFLPNW
                     TPETEIGGA"
     gene            complement(18630..19049)
                     /gene="fabZ"
                     /locus_tag="IV334_001099"
     CDS             complement(18630..19049)
                     /gene="fabZ"
                     /locus_tag="IV334_001099"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HAP8943186.1"
                     /translation="MNIQEIKEIIPHRYPMLLIDRVEEMVEGERIVAKKNVTINEPFF
                     QGHFPEEPVMPGVLIVEAMAQAGAVALLSLEQFKGKTAYFGGLDKAKFRKKVTPGDTL
                     YLEVEILKVKASAGIGKGIAKVDGKKVAEAELTFMIG"
     gene            complement(19074..19550)
                     /gene="accB"
                     /locus_tag="IV334_001100"
     CDS             complement(19074..19550)
                     /gene="accB"
                     /locus_tag="IV334_001100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HAP8943187.1"
                     /translation="MDINEIRELVSQFDQSSLTEFDLREGQFELYMNKNKTSRGMNMV
                     QTNETLPVSEPVNVIPEQATTAPEIMKEKESAVFDGIEVVSPIVGIVYLQPAPDKPAF
                     KKVGDQVAKGEVICIIEAMKLMNEITSEADGVITEILVENESVVEYGQPLFRIQQK"
     gene            complement(19553..20788)
                     /gene="fabF"
                     /locus_tag="IV334_001101"
     CDS             complement(19553..20788)
                     /gene="fabF"
                     /locus_tag="IV334_001101"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HAP8943188.1"
                     /translation="MNRVVITGYGVTSPIGNDADSFLNSLKEGTNGIAPITKFDASET
                     GITLAGEVKDFPYDKYFIKKDSKRMDMFSIYGIYAALEAMEMSGLDKEKMNQDRFGVI
                     IGSGIGGLPTIENQVIRLHEKGAKRVSPMFVPMAIANMAAGNIALRVGAKGICTAIVT
                     ACASGTNSIGEAFRNIKHGYSDVILAGGTEATICEMGIAGFAALTALSESTDPNRGSI
                     PFDENRNGFVMGEGAGVLVLESLEHAQARGAKIYGEIVGYGANCDAYHMTAPTPDGSG
                     ASKAMKLAMEEAGVQPEQVGYINAHGTSTPANDQAEAKAIQIALGENFRDTYVSSTKS
                     MTGHLLGAAGGIEALATLLALEHQFIPPTINVEKQDPEIDLTVVKNESKPASFTYAMS
                     NSLGFGGHNAVLCMKRWEE"
     gene            complement(20807..21544)
                     /gene="fabG"
                     /locus_tag="IV334_001102"
     CDS             complement(20807..21544)
                     /gene="fabG"
                     /locus_tag="IV334_001102"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HAP8943189.1"
                     /translation="MDVKGKNVFITGSTRGIGKAMALAFAKAGANIILNGRGEIPKEK
                     IEEIEAFGVKCVGVSGDISDYEKAGQMIKEAEEKLGSIHVLVNNAGITNDKLVMRMDA
                     EDFKKCLDINLIGTFNMTQHVLKKMMKQREGAIINLSSVSGLIGNIGQANYAASKAGV
                     VGLTKSVAREAATRGITCNAIAPGFITTDMTEVLADKVKEQAEKQIPMQRFGQVEDIA
                     QTAVFLAQNPYITGQVINVDGGLVMHG"
     gene            complement(21556..22473)
                     /gene="fabD"
                     /locus_tag="IV334_001103"
     CDS             complement(21556..22473)
                     /gene="fabD"
                     /locus_tag="IV334_001103"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HAP8943190.1"
                     /translation="MKTAFLFSGQGAQYQGMGKDLYEEAVVKQTFDEASEILGYDMAE
                     LCFTENERLDQTQYTQPAILTVSIAYYRLLKEHGIIPDAALGLSLGEYSALVASNALS
                     FTEAVALVAKRGAYMTEAAPAGSGKMVAVMNAPIETIEESCHEASKYGIVSPANYNTP
                     QQIVIGGEEKAVDEAVLLLKEKGFKRMIPLNVSGPFHTAILEPAAKKLAKDLSQIQFS
                     EPSFPIISNTTTEIMKKETIAGLLEQQVMQPVRFYESIHKLKTIGIEQVIEVGPGKVL
                     SGFMKKIDKTIPVLRVENKQTFDETIAIL"
     gene            complement(22580..22804)
                     /locus_tag="IV334_001104"
     CDS             complement(22580..22804)
                     /locus_tag="IV334_001104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HAP8943191.1"
                     /translation="MVFEKIQEIIAEELGKETEEIKLTTDIQEDLEADSLDLFQIINE
                     IEDEFDVKIETEDGIKTVQDLVTYVEKQTA"
     gene            complement(22857..23819)
                     /locus_tag="IV334_001105"
     CDS             complement(22857..23819)
                     /locus_tag="IV334_001105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketoacyl-ACP synthase III"
                     /protein_id="HAP8943192.1"
                     /translation="MEQFGMITATASVLPEKIITNNDLSKMMDTSDEWISSRTGIRER
                     RCVTNQETSDLCILVAEKLIKQKNLNPKDLDFILVATMSPDYTTPSVAAQVQGKLGAT
                     HAIAFDISAACSGFVYALSMAEKMIRCGSTKGLVIGGETLSKLIDWSDRTTAVLFGDG
                     AGGILLEANPQKKLLKEKLAADGTRSSSLTAGYQQNKNPFYSEDSEGSYYLQMTGRDI
                     FDFAVRDVANNIQEVMKDKPVDYLLLHQANLRIIEKIARKVKMPQEKFLTNMDKYGNT
                     SAASIPILLDEAVSSGKITLGKQQKVIFTGYGGGLTWGSILMEL"
     gene            complement(23829..24269)
                     /locus_tag="IV334_001106"
     CDS             complement(23829..24269)
                     /locus_tag="IV334_001106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAP8943193.1"
                     /translation="MEPDLKTVNDYLVSVFNDILTIEESELKKSQFKDLSITEMHTIE
                     AIGMYKKKTTSEVAKELSITVGTLTTAINKLVKKGYVERIRSEDDRRVVKLGLTKKGK
                     LLFRVHQHFHREMVKNILDGMATEEQHALLDALKNLHDFLQDYK"
BASE COUNT         6703 a         5445 c         4252 g         8084 t
ORIGIN      
        1 tttatttcga atccataaaa tattcttgga ttgcttctcg tgtttttttc actttttcgc
       61 taagcttttc gtttttagcc atatagaaat aaaaaatgat atcgatcgta gcaaattggg
      121 ccaaaagtga attagttgcc gcacttcgat tcgtgatttc ttttggtcta gatgtttgta
      181 cgagtacatc ggctatcttg ctaagcgggt tatttccaaa ttgcgtcaac gcaatgattt
      241 cgatattcat gcttttcgcc agctcggcta atgcgactac atctgcactt ctacctgagt
      301 ttgaaacaag ccaaagcact ttctttttct cattgctgac aagttgcggg agcaagacgt
      361 ggatatcttt ttcaaaaatg attggcttac cgacacgaga ccatttttgc agaatatctt
      421 ctactactaa agaagaggcg ccaaccccat ataaaaaaac agtctctgcc gattccaaac
      481 actctacgac tttttggatc gtttcttctt ctagaatatc tactgtttct tttatcgcta
      541 cttgtgcatt gttaccaagt ttgtttttga tagagtaaaa ggactcattt gcttctatat
      601 cactaaaacc ttcatcgatt ccagtctgta gttctgttga aatcgtgatt ttgaagtccg
      661 tcagactttg aaaaccgatt tttttggaaa aacgtacaac tgtcggcgca ctcaacccgg
      721 aagcttctgc taattcatta gctgtcattg tagggacctt ctcaatatta ccgataatgt
      781 agttggccaa ctttttttct gctgaagaat aatcttccat catactttca atacgactaa
      841 ttacgctcat aagttcctcc acccttttaa tgatagtcta tgtaagtata ccatatcaga
      901 cccttattca cggtcgtata ctggataaaa aacaaaaaaa caatccgccc aacagaatat
      961 cttatcccat caatcccaca gattctattg agcatctgtg tctttgagga atacctgtta
     1021 tctattgttc ggattgcttt ctatctacta cttttttaca ggaaatttac ctattagttt
     1081 tctctgctgt aatttttgac ttctgcttcc atagcatcaa taaagatatt caaatcttct
     1141 acagctgttt ctttacttcc ataacgacgt acgttcactg ttgcatcttc caattccttg
     1201 tctccaacaa ctaattggta agggatcttt tgtgtttgag aggcacgtat cttatagccc
     1261 attttttcat tacgatcatc aacttctaca cgcaacccct tcatttgcaa acgttctttg
     1321 atttcataag cataatcgcc atgtgcatca acagaaacag ggatgattgt tgcttgtata
     1381 ggtgccaacc atgttggaaa agcgcctttg tatacttctg ttaaataagc gacaaaacgt
     1441 tccattgtag aaacaattcc gcggtggata actactggac gatgcgtatt ttccccgtct
     1501 tctccaacat atgtcaaatc aaagcgctct ggcaataaga agtctaattg tatagttgat
     1561 aatgtttctt ctgtaccaag agctgttttg acttgaacat ctagttttgg tccgtaaaag
     1621 gctgcctcgc cttctgcttc aaagtaatct agttctaatt catccatagc ggctttcagc
     1681 attgcttggg ctttttccca cattgcgtca tcatcaaaat atttatctgt attgtttgga
     1741 tcgcgatagc tcaagcggaa acgataatct gtaatattga agtcagcata tactgccacc
     1801 atcaattcaa gcgtacgttt aaattcatct ttgatttgat caggacgaac aaatgtatga
     1861 ccatcattca acgtcatttc tcgtacacgt tgtaaacctg acaaagcacc tgatttttca
     1921 tagcgatgca tcattcctaa ttcagcgata cgaattggta attcacgata agaatggatc
     1981 gtgtttttgt agaccatcat gtgatgaggg cagttcattg ggcgaagaac aagcatttcg
     2041 ccatctccca tatccattgg cgggaacatg tcttcttgat aatgatccca gtgtccagat
     2101 gttttgtaca actcaacatc tcccattatt ggtgtatata catgttggta acctagacta
     2161 acttctttgt ctacgatata acgttcgatc gtgcgtcgga ttgttgcccc ttttggaagc
     2221 cagaatggca aaccagaacc tacttctggg gaaaccatga acaagtcaag ttcttttcct
     2281 agtttacgat ggtctctttc ttttgcttct tcacggagac ggatatattc tttcaaagct
     2341 ttcttgtcaa aaaaggctgt cccgtataca cgttgcatca tttgattgtt tgagtttcca
     2401 cgccaataag cacctgccac tgataataat ttaaatactt gtatacggcc agtggatgga
     2461 acatgtggtc cacgacacaa atcgatgaat tcgccttgtt gataagcagt aatcacttct
     2521 tcttctggca attcactaat cagttctact ttgtacggat cacttgcaaa aatttctaat
     2581 gcttcctgtt ttgaaagaac acggcgttcg atcggcagat tttctttgac gattttcatc
     2641 atttctgctt cgattgccgg caaatcttct gcagttactt ggttttgtcc attatctgta
     2701 tcgtagtaaa accctgaatc gatagctggt ccaacgccaa agtggatatt tggatagagg
     2761 cgacgcatgg cttgggccat caaatgagct gctgaatgac ggactaagcc aagtgcatct
     2821 tcatggtctg gtgtgatgat ctcaatactt gcatcttcct cgattggacg agtcaaatca
     2881 acgagttttc cgttcacttt accagctaaa gctttttttg ctaagctttt tgaaatgctt
     2941 tcagcaattg ccaatgttgt tgttccagac tcaaattctt tgactgcacc atctggaaaa
     3001 gtaattttta tcataatagt ttcctccttg aaatgttttg attgatcaga tcatcaagct
     3061 gaaaaacaaa aaaatcctag catctcttga aaagagatac taggacgaaa agttcgtggt
     3121 tccacctaat gttcaaacgc aaatgcgttc ctcaagcgtt gtaacggtcc gaccgccctt
     3181 gcttcaacaa gggtttcgaa agtggtcatc ctttggtttt ctcctggaaa agtttcagcc
     3241 tagcttttcc tctcttatcg aagactctca aattcagttg gctttctcat caatcaatta
     3301 tgtgtttatt taaccactaa taaatagaaa aatcaagtgg gaaactaaac ttattttttc
     3361 cattccccat ttacaccgta tgttcctttt ttcatctcat tgatgatgac atggatgtgt
     3421 tcttttggtg cctgcgtgtt tttatgaaca gcttccgtga tatctttcat catattttct
     3481 aattgttccg gactgcgacc ttctactaat tcgacatgta cgaatggcat aatgctctct
     3541 cctctttctg atcgttcttt tttcaactcg attctatcat actatgtttg tcttgggtac
     3601 tgaatcagga aaaatggctg gatctttttt tgctctcaag gcactcaaaa tagagaccgt
     3661 atccacgact tcttgcaaaa cagctccgaa taatgcgggg attagtcccg tacttgcaat
     3721 taacatcaaa aaaatacaaa taaagatgcc aatcagtaca gactgtttcg ctattctcat
     3781 cgtatctctt gaaatcacta ctgcttttgc tacacgcaag agatcatctt tcaagatgac
     3841 cgcatctgca ctttcactag cagctgtaga accatgagcc cccattgcaa taccgatatc
     3901 tgcagcagca agtgctggtg cgtcattcac gccgtctcct accatcgcaa caggtctttg
     3961 atctttaggg atactttcta aaatggtcag tttatcttga ggcaagctac gggcatgtac
     4021 agaatctaat tggacttttt ctgcaatcgt ttgagcaatg gcagatccat ccccggtgat
     4081 catcatcaaa tttttcacat caagttttct tagctccttg attgtctttg ctgcttctgg
     4141 gcgaatctga tccgaaaata ggatcgttcc tgcaaaagat cgatcaattg ttacatagat
     4201 tgctgtattg gacgtttcta tttcttcagc tgaaacaaaa gaggcacgtc caatctttac
     4261 ttcatgatta ttgactactg cttggattcc ttgaccggtc acttctttca gttgactaac
     4321 aggatacagt tggctgtttt tcacgttgtt cactaacgaa cgagctaaga tatggctaga
     4381 ttcctgttct gcactagctg catacagaag tagctcttct ttttttattc cattgactgg
     4441 ctgaatcgcc tctacttcca atatcccttt ggtaagcgtt cctgttttat caaaagcaat
     4501 cgtttttgta cgtgcgagct tttcaatcgt tgttccagtt tttacaacga tcccgttttt
     4561 acttgaacgg ctcattcctg caactaaagc aatgggcgca gctagaatca gtggacaagg
     4621 tgatgccact accagtacct cagcaaatcg taaagggtct tttgagaacc accaagaaat
     4681 accaccgatc aaataagcaa tcaacgtgaa agggactgca taacgatcag ctaaacggac
     4741 aaatcgggca ggcttttctt ttgattcttc gactagttta ataatcattt gatactggct
     4801 gtctgcggca cgtttttcag cgatcatttg gatcgatccg tctccattta aagaccctga
     4861 cataatctga tctccaggtt tcttggtttc cgggcgggac tcacctgtca aagaagattc
     4921 atcgaccgaa gattctcctt tggtcactcg accatctgcg ggtacaagtt cgccaggctt
     4981 gactagtaac tggtcatcaa tatttatttc atcgattact atatcctgca attttccttc
     5041 tttgaatcga tgggcaattc ttggggtgtt atctaacaaa gactgcagtt ctttacccgc
     5101 tcgtttattc gcgtaatcct ctagactatc tccgcccgta agcatgatca atatcatcaa
     5161 actagcccag tattctttga cggctaatgt tgcaaggatt gcggtaatcg ctaatacatc
     5221 tacgccatat cttcctgaac gtaacgtctt gatcatttcc aaaaacatca agaaggcgag
     5281 aatccctcca gtaaatgcga caatcgcaaa tgcaagggcc ggctggtgaa aaacaaattc
     5341 acttaataaa gctatcccac caataagcaa agtaagtaaa aattttttga aatgtgtcat
     5401 tgttttttct cctcttttat gtgacttaga gaggttagta ataaatttcc agctgcctct
     5461 ttcatcttaa gcatagtcct tgactagcac tttttaaaac aataggcctt aattgaaaac
     5521 aactatcact atcagcgttt ccaaaattca tcagttctac aaacaaaaat attcagcaag
     5581 gattactttt tcgattcatc agttaaaaag tacctttttt cgaaaaacta tgctatactt
     5641 ttaattcgtt atttacggcg gaaggagcaa acatgcgatg aatgagaaac aactagaaca
     5701 aacgcacgtg gatgaaacga tcgaactaat ccagatcgaa caaaatatgc tggatacaaa
     5761 acaacaagaa ttgactcagg aaatgaatca ttcgacaaaa gacacagcta atcacaagat
     5821 tcgagctggt tccaatgaag ctttttatga atctgctgtt gaatatcgcc aacatgagca
     5881 ggaattgctt ttgaaatacc atactcttga agcacagcaa aaacgcctgc aaacgttgtc
     5941 tgtcatgaaa gatagtcctt attttgcccg tatcgacttt aaagaagacc gtgaaacaga
     6001 aacattatat ctaggtatcg cttctttaag agatctggca gaagaaacaa tcgttattga
     6061 ctggcgcgca ccaattgcca atctttatta tgaaggtgag attggtcccg cattttatga
     6121 aactgaaaca gatagaatag atgtcgatct gcagttgaaa cgacaattta aaatccaaga
     6181 aggacgtatt ttatctatgg tagatacgtc ggaagtaatc aatgatgact ttttgctaga
     6241 gatattggat gaatcatcaa gcaaccatat gaaaaacatc gtctctacga tacaaaaagc
     6301 acaaaatgca atcattcggg acacaacaag taaagtaatg ctgattgaag gaatcgccgg
     6361 aagtggaaag acatccgctt tgcttcaacg tattgctttt ctgttgtatc ataatcgcaa
     6421 gtggctcgag gctgaaaacg ttcttctatt ttcacccaac catttgtttt ctgattacat
     6481 ctcgactgta ttgccttcat taggtgaaag cggcgtgcct acacagacat tcaaaaatta
     6541 tttggtgcag ctgctgccag agttttcttt actagatgaa cagcaacaag aaagtggttt
     6601 tttgctaggg gaaaaagatc cgatacaagt gatgaaaagc ggattgacct tagtcgatca
     6661 gattgatcgc tatatccaag gcatcacaaa ctacggtccc ttgttccgtg atatgaaaat
     6721 aaatggccgt acgatcctat caaaggaatc gattcgccaa tggtataaag aaacaaatga
     6781 acaattacct ttacatcata gactttctct attacaaacg aagctcctga aaaaattagg
     6841 cggtctgcaa aaagacgaaa cacgtcagca atgggtcaaa gatctggcgg aagaacagct
     6901 ccaagaacta tacgcaacag atccaaatct tgaatataca gaacaaaagg agcgttcatt
     6961 acgtaaaaaa ctggccaacc aaatcgtacg taaaagattc cgtcgaatcc atcgagggat
     7021 catgaattat caatttgtca atataacgaa acaatatctc cactttctac aaactgtgcc
     7081 actttctctt ctaacacgac ataaaatcag cgagaccagg tggcaggaac atttgacaac
     7141 tgttcgtaca cacttgcgtc aaagagaatt gcgtcaagag gatgctgtac tattcttttt
     7201 actgatgcgc cgaacatatc cagtgacggt tacacacaaa gctcgctata tctttattga
     7261 tgaaatgcag gatttcgcac catcacaagt ggcattgctg caagccttgt tcccaaaagc
     7321 aaatttgact ttctgtggag atctaaatca aaatgtcttt ggaaatgaaa cgatcgttgg
     7381 atcattggaa gaccttttcg tggaccgtga agtcactcgt ttccaattaa cgacaagcta
     7441 tcgctccaca aaagaaatca ctgattttgc taatcatttt attgtgaaag aacatcaagt
     7501 agaaacaaca gcaagaaaag gagaccttcc tttgctcatc caaggaacaa cagatgatat
     7561 caaattgaat tggttagcag ataccattcg aacgactcag ggaaaagccc ggtattggcg
     7621 tacagcaatc atagctaaaa cacaagaaga atgcgaagct ctttatgctc aactgccaga
     7681 cgagttgaaa caacaagtcc agctgatttt aaatgagagt gactttatga agcgttccat
     7741 tattttactt ccagcctttt tagcaaaagg tctggagttt gaccgagttt tcttgtggga
     7801 tgtagatgca gaacattttt cttctcagca agacaaattg atcctttaca ccatgtgtac
     7861 acgtgcgatg catgaattga ccctttttac ctccaataat gaaagtcctt tgttaaacaa
     7921 tatggatatt tcatcttatg agaaaaaagt actgtaagta aaaagacaac tgctatcatt
     7981 aatgttagca gttgtctttt tatttacaat aataattgca gaaaaagtaa ttcaatcatt
     8041 taaggtcatt tattctatat ataaatccaa atctgtttca ggatatttta atttgacaag
     8101 cgaaataatc attttcagtt cttccatgtt ttctggatat tctgggtcaa ataccttgct
     8161 atgacggcca atcccgccat tgtcgatcaa tcggtaaatt cgattccatt caatgaattg
     8221 gtcaattgat tggaaattgc tgattggata gccgccacaa tccaaaatct ctgatacacg
     8281 aaatgctagg tatgcactat cgatcacttt attttttttc gtgtctatct tctcatttct
     8341 ttcgataaga attttttcag gacgatgttt caagctccac tctaatagta gaaagaaagc
     8401 ctccatccga tactcttctt caaataaccg aaaggctgaa tcagaacgac gaaaaagcag
     8461 cggaatcaga taattttgtc tataaatcag cattggtgcg cggtccacca tacactctaa
     8521 ataacggttc agataccctg taccgacata gtttcctaat cgatagcctt caacgccgaa
     8581 tcttgcgtat ttttgcgcta attttgaaca atcagttacg ttcacagcta actcttctac
     8641 tttgcggtaa ccgcttaaga tacgttcaaa aggttctaaa gtgatttttg cttccatatt
     8701 cttccccgtc catcttccac atacttcata tcatttcaca tgatacttat gctctcatca
     8761 taccacaggt tgaattagaa aagaaaataa atcacacaaa aaagcaagac aatctgttag
     8821 tgagattgtc ttgcttttcg aaaaagactg aatacttttc gttaaaactg gttgcttagt
     8881 cttctaatgc cacattatgg aacacatgct gtacatcttc tagatcttcc agtgcatcaa
     8941 tcattttttc aaatgtttct tgatcttctg gagatagtgt tacctcgttt tgagggatca
     9001 tttggatctc agcgattgaa aaatcagtga ttcctttttc ttttaatgct tcttgtatac
     9061 tatggaaatc cgctacttca ccgtaaacga tgatttgtcc ttcttcttcc attacatcgc
     9121 gaacatcgat gtctttttcc attaggtatt caaggatctc gtcagcgtct tctcctgcaa
     9181 aagcaaagat tgctgtattg tcaaacatgt aagaaaccgc tccggaaaca cccatgttcc
     9241 cgccattttt cccaaatgct gcccgtacgt ctgctgctgt tcgattcaca ttgttcgtca
     9301 atgtatcaac aatcaccatt gagccgcttg gtccaaatcc ttcatagcgc aattcagagt
     9361 atgtttcatc tcctgcacct tttgcttttt cgattgctcg gtcaataata tgttttggta
     9421 cattataagt tttagcgcga tcaatgacga aacgtagttt ttggtttgca tgcggatcag
     9481 gatcacctga ttttgctgcc gcgtagattt caatcccaaa ttttgcataa atccggctat
     9541 tattagcatc tttagcagtt ttctttgcta cgatatttgc ccatttacgt cccatatttt
     9601 cacttcgctt tctgatttga tagtttcata acgcaaaaac gtcctgttcc attatacttg
     9661 tgaatcacga ttctgtgaag ctttttttct ttcttccttc attcttctca tttttagtca
     9721 attatctata ctcggattgg gatttgcccg aaattctgtt ccggaatatt gcctcccaat
     9781 tgatagattt gatcacttct aactaacagt tccgcttctt gtgcctcttt ttttccgatt
     9841 cgggaataaa tagaaatctc ttttttttct tttaagaatc ttcttccagc atcggtaaat
     9901 ccaagtagtc gaagataatt tttattttgt tggacaggaa tctcttgctt tttgatattc
     9961 agcaagacat aacaagataa tcgttggatc cgtgtccatg tatatctttt tgtttttacc
    10021 tttgacacaa aatcctcaaa actatcagca gttgcagctg ctgttttcat gcgcgattct
    10081 attccttcgg tcatctgata gatatctgtc agttccagca aagtagaaga taacaaccga
    10141 taacgcaaat acggccaata agattcccaa gtctgcagcg aacaagttga aagatcttct
    10201 tctgtctgag gcggcacaag tccagctatt ctttttcctt cttctaacgc atggcgaatg
    10261 gctgttgcac tggctatcct ctgatctgtg atgatttttt catgatagcc tgcactttta
    10321 cgtcggatcg gatgtaattc catagggtgg acatacttgg cattttcctt tgcataactt
    10381 aatcccaaga tatgatttgg cgaagaaaaa tcaagcgtaa tatcgggata cagactgcga
    10441 aaaacctcag tcattttttg cggataacta tacgtttgat cagacagttc ttgaaatgcc
    10501 tcttctatcc tatcttgatt ctcttggaca aagcgtccga aactgctgta atcaaacgaa
    10561 gtctcttctt ctgttccaaa acacaaactg tcacagtcca gtgtttgcaa gatttttact
    10621 gcacctttag caaaatagtc agctgactga accgcccatt cgacgggaag ctccactact
    10681 aaatccacac cattttgtaa agcttcattc gcgcgatgcc atttatcgat gatagccggt
    10741 tcgcctcttt gcaaaaaatt cccgctcatt acagcaatga cgacctctgc tcctgttatt
    10801 tttcgtgcca tttccacatg atagcgatgt ccattgtgaa aaggattgta ctctacgata
    10861 atcccgcagc ttttcatgcg catcctcctt catagttatc gataaaaaag aaatagagac
    10921 aagtaaaaaa aggcaaaaat cggaaaaaat acccgaacta tataaactat tctcgcaaac
    10981 acaatacttc tgcgacgaaa agctgcactg caagagcaat gcctatatcg ttcggatata
    11041 taatttatgt tgaagattta ttcttttctt gttaactcga gcaggcaaaa ccgctagtct
    11101 ttgtatgcta caaagaacca gcgctttgat ttctctgatg ctgcttcgtc tgtaaaatca
    11161 gaaaatgctt cgactttcga aaagccgctg ttttccagca ttcttaaata actttccaat
    11221 ggataggtac gctcttgatg tagttcatct tcacgaataa atcgaccaga cccttcttgt
    11281 tcttcaacga aaaaggtaag aaaatgttcg atgctgtgtt tctcgcttcc tggataacta
    11341 tcccacaaaa aagcaaaatt atccccttga taatgatagc tatattctgg aaaaacctca
    11401 tctacttgat aggtggagtg tacgtcaaat aaaaatcggc cattttcttc taacgcttga
    11461 tagacttcat caaacacttg ctgtacctct tgtctatttt tcatatagca cagcgaatca
    11521 gaaaaacagg taattgcctg atattgtccg atttctgata aatcgagcat attgccttcc
    11581 acaaattgga tcgatgcgtc ttcttcatat gctcgctggc tggcaatcat caacatctct
    11641 tctgaaaggt ctaaccctgt cactttatat ccatcttttg caaaggcaac cgctaatgct
    11701 ccagtaccgc atgccagttc caaaatattt tttgtgtctt cttgtaaatg acgtttcgaa
    11761 aaagctaacc attgctgata aagtgtttcg tccatcactt catcgtaaat gaaagcaaat
    11821 gtttcataag ccatatgtta atccaaccat tcactaagat cgactagcgg agcatctgac
    11881 catagttttt ccagattata gaaatttcgt tctggcgcat ggaaaacatg tacgatcaca
    11941 tctccaagat cgatcaatac ccattttccg ccttcttttc cttcaatacg ttttacttca
    12001 tatttgtttt cttcttcttt atcaatgatt tcttctgtaa ttgcgttgat ttgacgttcg
    12061 ctatttgctg aacagatcat gaaataatct gccaacaagg aaacatttcg aacgtctaaa
    12121 gcgataatgt cttctgcccg ttttgaatct gctgcttcaa tcgcaatttt taattgttct
    12181 ttactatcta tgatgatttc ctcctaatat gagcagctta tttttttgct acccagtaat
    12241 tatatgtttc gattgttttt ggataaatct gttgttcttg cgcaagcaaa tgggccaatg
    12301 tatgttttgt ttcaaatgct acagcttcgt ctagatcagc ccatgcaatg gctcgtgcat
    12361 cttgtacccc aggaaaatct cgacctggtt caatatagtc tgctacataa atgattttgt
    12421 caaggagact catcttagca gccccagtcg tatgcacccg gattgcatga agaatctctg
    12481 catcggtgat tcctaattcc cgctctacaa acgaagcgcc aacaagacca tgccagattg
    12541 cattattata attcaataac tccggatcaa aaccgtctct tacgattacc attttgaact
    12601 cctcatctgg tcgttctttt gcataatcat gtgtcaatgc agcaatacta gctttttcag
    12661 gagaagcacc gtattttttg gcaagagcca cggctgtttc ttctactcca agcacatgct
    12721 tgaagcgttg ttcgctcatt ctcatctgta ttttttgcat caggttttct ctagaataag
    12781 cagtatattc attactatag tccatattca tacaaccctt cattttgaat ataatcaatt
    12841 actttatctg gaacaaggta gcggatagaa cagccttctt tgatcttttg acgtattttc
    12901 gtggaactga tgtcgatctc aggtacatct acccaaatta caggatatgg agtatccgtt
    12961 gtgtatcctg gtcgacgtat acccacaaag ttgaccatag aagtcagctc atcgatttta
    13021 taccattttg gcaaatattc taccatatcc ccaccgataa taaagtaata atctgtatcc
    13081 ggattgtttt gcgtcaattc cttcatagta tcataggtgt aacttttccc accacgagct
    13141 aattcaatcg tttcaatctg caaaaaagga ttgtcttcca ccgctaattc caacatgttg
    13201 agtcgatgtt ttgcatcaat cgtctgtttc tcatcaacat gaggtggctg ataagaaggc
    13261 atcaaaaata cgcggtcaag acctaaattc cggccagctt gttcagccat cactaaatgt
    13321 gcaaggtgaa cgggattgaa tgttccgccc aagatcccta cttgcttttt cttctctaag
    13381 aattgaggca tttcttcagg aaataattgt gaacgaacaa atgtcttagc ttgcgtattc
    13441 atcatggtca ctccttatat tgcttttact tcttttgata gcctttgata cttttctttt
    13501 gtggaaggtt tgtataatac aataacacgt ccaatgattt ggacgatttc gcaatctatt
    13561 tctttttcta atacttgagc tgcttcttct gcaatctcat ctgtattttg aagtaacgat
    13621 actttgatca gttctctttt ttctaaagct tcttcaattt gaacgatcat tgctgaattg
    13681 atcccacctt ttccgatttg aaaaatcggc tgaagatgat gcgctttgct acgtaaaaaa
    13741 cgcttttgtt ttcctcttaa actcataatt ttccttcttc cttaaatcaa tgctttgcgt
    13801 ttcaatacat ctacaccctt tggcgcccag cccgcaatta ctactggcgc tgttacagtg
    13861 atccagccta gaccggcaaa aacgatgtct gttttttctt tcacagaaaa ctcaaaacga
    13921 acaagctccg gaaatgcctc gacttcatca ggacgaggag gctgtaataa tccacctaca
    13981 tgtttttgat aaaattcgtc tgctttttcc agtttcgttc gatgaatatt tacatcattt
    14041 gacacataag caataaatga agcacgattg ccgctgataa aatcaaatct tgcaagtccg
    14101 cccaagaaaa gtgtttgccc ttcattcagc tgataaactt taggtttgat ttcttttaac
    14161 ggtgcaatga tccgcaaatc ttttttacct aagtagtgcg ccatttgatg gcgatggata
    14221 attcccggtg tatcaatcaa aaaatgcccg tcatctaaag gaatctcgat tttatccaag
    14281 gtcgttcctg gaaactgcga agttgtgatt acatcttgta ctcccgctgt atttttgatg
    14341 atttgattga taagcgtcga ttttccgaca tttgttactc cgacgatata gacatcccgt
    14401 ccatcgcggt attcttcgat tttttctaat agttccgtca tctcatgaga tttcttagca
    14461 ctagttaata atacatctac tggacgaatt cctgcttcgt aagcacgttc cttcatccat
    14521 tgtgttaatt tagttttttt caatgattta ggtaatatat ccactttgtt cccaactaaa
    14581 agtactggat tgtcaccgac aaaacggtgt agtcctggga ttagtgaacc attgaagtca
    14641 aaaatatcca ccacattgac gatcaatgca tctttttgcc ctaattcatt cagtaagcgc
    14701 aaaaagtcgt catccgtcaa tgagacatct tgggtctcat tataatggcg taatctgaaa
    14761 caacgctgac aatagacttc cccggaagct aaaccttttt ccaaagccgt ttgcggcgtg
    14821 tatcctaatt caccgggttt gtttgtttgt ataacggcac cacagccgat acattgtaat
    14881 tcttctgtca ttcgattcca cctcgccagc ccatatcagg gtgttttgct aatagatagg
    14941 ccataattct tttttcccaa aaacgattaa attgtgtctt ccaactatct gttgcgataa
    15001 tgggttttac aaggatgcta cgtatccctg cacgattggc tccacgtata tcagtcataa
    15061 tctgatcgcc taccattact atctcatcat ctgctaaatc caattgtttc tttgctatgt
    15121 tgatccctct tgctaaaggt ttcattgctc gtgcaacata caaaagatca aatttttcaa
    15181 tcgctcgcgc gacacgtttt gaattattgt ttgaaacaac aacaacaggg attcctgcat
    15241 ttttcatttc taaaatccaa gtaagcaact cttctgttcc atctggatta ttccatgcaa
    15301 tcaacgtatt gtctaaatcg gtcaatactg ctttgatgcc tagattcttt aattgggcag
    15361 gtgtaatctg atagatggct tctaccatcc atgttggttt gtaatttgaa aacatgctgt
    15421 ctcctcatct gcaaaaaatg aaggagcaca taaagtactc ctcaaccccg tattatactg
    15481 catatcagca aaaaaaacta cctctatact cgttcgtctt tcagaaaaag catttcttta
    15541 caaaatgacc aaaaataaag attgatcaca gaaaaattta gaaacaaaaa acaactaata
    15601 ttggcggaac ctcttgtatc gtttttccag taatttatgc tgatctaatc gaacaagttc
    15661 cgtcaatctt tcaatcaacg cttttttcat aatgtgtgcg actttatatc gtggcaactg
    15721 ctcgccgttg aacgtttcag gaataactcg atcaacgatt cctaactcgc gtagttctgg
    15781 cgcagtaatt ttcatcaact cagctgcttc tgcggcgcga ctaccgtctt tccacaaaat
    15841 cgacgcaaat ccttctggag aaagcacagc ataaatcgtt tcttccagca tccagacttc
    15901 atccgctaaa cctagagcaa gggcaccgcc acttccacct tcaccaatga tgatggcgat
    15961 gatcggtacc tttaaatcag acatttcgag cagatttctg gcaatagcct ctccttcgcc
    16021 gcgctcttct gctcctacac cgcaatatgc acctgctgta ttgatcaagg taatcaccgg
    16081 tctattgaac ttttccgctt gtttcatcag tcgtaaagct tttcgatagc cttcaggatg
    16141 gggagaacca aaatttctgc ggatattttc ctgcagatcc cgtccttttt gaataccgat
    16201 cactgttacc ggtttgccat ctaatcgagc gattccacca acaatcgctt cgtcatctgc
    16261 aaataaacga tcaccatggc attcctcgaa agaatcgaag atctcttcaa aaaaatccaa
    16321 gcttgtccat cgttctttag ctcttgctaa ctgaacgaca tcatgtgctg tcttatccac
    16381 gatctatcca tcctttctgt gtatgcaatt caagaagcgt atggattttc tgtttcaatt
    16441 ccatgcgggg aacaatctga tcgacaaaac catgagataa taagaattca gccttttgga
    16501 aatcttctgg aagttcctgc ttgatcgtct gttcgatgac gcgtcttcca gcaaaaccga
    16561 tcaagctttg gggttctgct agaataatat cgccttccat tgcaaaactg gcagttactc
    16621 ctccggtcgt cgggtccgtt aaaacagtta gataaaaaag accttcattg ctatgccgtt
    16681 tgacagcagc agagattttt gccatctgca ttagagaaaa gattccttct tgcatccgtg
    16741 cgccacctga agctgtgaac aagacgactg gaagactttc ctttgttgct cgttcgaata
    16801 atctggtgat cttttctccg acaatcgttc ccatacttcc catgataaaa ttaggatcca
    16861 tcactccaat aatgaaggga atcccctgaa tggttgcttt accagtgagg actgcttcat
    16921 gaagtcctgt tttatcttgc atttttttaa ttttttccgt atattcggga aatgctagcg
    16981 gatctttggt tacaagatcc gtatcccatt cttcaaaact tttttcatca ataatcaaag
    17041 ctagacgctg ccatgcaccg attcgaaaag cgtatccgca atgagggcat actttttctt
    17101 caccgatgtc tttcgtataa aggatatgtt tgcaatttgg acactttgcc cacatgttat
    17161 caggaacaga tggcgagtta gcaggtttgc tctctctatt aggattgatc cgaatatatt
    17221 tctttttctt aaacagagcc atctatgcgc ctcctatctc cgtttctggt gtccaattcg
    17281 gtaaaaattc ttcttgtaaa aatgctgtgc tgtaatcacc tgctatgact gcaggatgac
    17341 taatcaaatc catctgaaat tctgcgttcg tgatgattcc ttctgtgata aattcactta
    17401 aggctcgctg catcttcatt aatgcttcaa aacgcgtatt tccatggacg attatttttg
    17461 caatcattga atcataatat ggcgggatcg tatatcccgg gtaaactgca ctgtctactc
    17521 gcagtcccat cccacctgct ggaagcagta agttttggat cttccctgga gaaggagcaa
    17581 aatggaaagc aggattttct gcattaatcc ggcattcgat tgcgtgtcct tggaatacta
    17641 cttcttcttg acacacattc aaaggctcac cggctgctat ctccacctgt ttttttacta
    17701 aatcgattcc tgtgaccatt tctgttactg gatgttccac ttggattcga gtattcattt
    17761 ccatgaaata aaaatcgcct tcttcatcca tcagaaactc gatcgttccg gcattttcat
    17821 aattgactgc tttagctgct cgaacagcag cgtctcctag tgcttctctt tttgtttgtg
    17881 agatcactac agaaggagat tcttccagta ctttttgatt gttgcgttgt aaagaacaat
    17941 ctcgctcgcc taaatgaatg acatgaccaa atcgatcacc taagatctga acttctatat
    18001 ggcgagcagg ataaatgatt ttttccaaat acatatcatc atttccaaat gccgctgctg
    18061 cttcttgctg agctgaagaa aaatgctgtg ggagctcttc ttttgattga actttccgaa
    18121 tgcctttccc gccaccacct gcagctgctt tcagcatcac tggataacca attctgtctg
    18181 ctatttccaa tgcttcttct actgtagata atacaccagt acttcctgga atgactggga
    18241 catttgcttc ttgcatcaat tgacgcgcat tgatcttatt tcccattgca tcgatcgttg
    18301 cacttttagg accaataaat gtgatattgc actcttcaca catggaggca aatcggctat
    18361 tttcagataa aaaaccaaat cctggatgaa ttgcttcggc ttttgtcacg attgcagcgc
    18421 tcagtacttc ctgaacattc agatatgaat caacggcttt tgccgggcca atgcagattg
    18481 cttcatctgc taactgtgta tgaagtgctt cggcatctgc ttctgaatat atcgctacgg
    18541 tttggatccc tagttcgcgg catgcacgaa tgatacgtac agcgatttca ccgcggttag
    18601 ctatcaatac tttagaaaac atatttcacc tatccaatca taaaggttaa ttccgcttcg
    18661 gctacttttt taccgtctac tttagcgatt cctttgccta ttccggctga agctttcact
    18721 ttcaatatct caacttcaag gtataaggta tctcccggtg taactttctt acggaatttt
    18781 gctttgtcca atccgccgaa ataagccgtc tttcccttga attgttcaag agaaagtaat
    18841 gcaactgctc ctgcttgagc cattgcttca acaatcaata cccctggcat aacaggttct
    18901 tctggaaaat gtccttgaaa aaagggctca ttgatcgtta catttttctt agcaacgatc
    18961 cgttctcctt caaccatttc ttccactcga tcaatcaaaa gcattggata acgatgtgga
    19021 atgatttctt ttatttcttg tatgttcatt gttttccctc tttcctgctt tgcttatttt
    19081 tgctgaatcc taaagagtgg ctgtccatat tcgacaactg actcgttttc tacaaggatt
    19141 tctgtgatca ctccgtcggc ctcactagtt atctcattca tcagtttcat cgcttcaata
    19201 atgcagataa cttctccttt ggcaacttga tcacctactt ttttgaaagc tggtttgtcg
    19261 ggcgctggtt gcagataaac gattcccacg attggcgaaa caacctcgat tccatcaaat
    19321 actgccgatt ctttttcttt cattatttct ggagcagttg ttgcttgttc aggtatgaca
    19381 ttgactggct ccgaaactgg caacgtttcg tttgtttgaa ccatattcat gcctcgactt
    19441 gttttatttt tattcatata caattcaaat tgaccttcac gcaaatcgaa ttccgttagc
    19501 gaggattgat caaattgact gactaattct ctgatttcat tgatatccaa aattattcct
    19561 cccagcgttt catgcacaga accgcattat gtccgccaaa ccctaatgaa ttactcattg
    19621 cataagtaaa acttgcaggt ttgctttcgt ttttgacaac tgtcaagtca atctcaggat
    19681 cttgtttttc tacattgatc gtaggtggga taaattgatg ttctaatgcc agcaaagttg
    19741 ccaaggcttc gatcccgccg gctgctccca ataaatgtcc agtcatcgat ttcgtgctgc
    19801 tgacgtaagt atctctgaaa ttttcaccta atgcgatttg gattgctttt gcttctgcct
    19861 ggtcattggc tggtgtactt gttccgtgag cattgatata tccgacttgt tccggctgta
    19921 caccagcttc ttccatcgca agcttcattg ctttagatgc accagaacca tctggagtag
    19981 gagctgtcat atggtaagcg tcacagtttg caccgtatcc aacgatttct ccatagattt
    20041 tggcaccgcg agcttgtgca tgttctaaag attcaagaac tagaacgcct gctccttcac
    20101 ccatcacaaa accattccga ttttcgtcaa acggaatcga accacgatta ggatcagttg
    20161 attctgagag tgctgttaaa gctgcaaatc cagcaatccc catctcacaa atcgttgctt
    20221 ctgttcctcc agcaagaatg acatcagaat agccatgttt gatgttacgg aatgcttccc
    20281 caattgaatt cgttccgcta gcacaagctg ttacgatagc tgtacaaata ccttttgcac
    20341 ctacacgcaa agcaatattt ccagctgcca tgttggcaat cgccattggt acgaacattg
    20401 gagaaactcg ttttgcacct ttttcatgta gacgaataac ttggttttcg attgttggaa
    20461 gaccaccgat cccagatccg atgatgacgc caaaccggtc ttgattcatt ttttctttat
    20521 ctaaaccgct catctccatt gcttctaaag cagcgtagat tccatagatc gaaaacatat
    20581 ccattcgttt gctatctttt ttaataaaat atttatcgta agggaaatct ttgacttcac
    20641 ccgctaaggt aatccctgtt tctgaagcat caaatttagt gatcggtgcg atgccattcg
    20701 tcccctcttt taagctattc aaaaagctgt ctgcgtcatt tccaataggt gaagtgactc
    20761 catagccagt aattaccact cgattcattt gtttgctctc cttttcttat ccgtgcatga
    20821 ccaatccgcc atcgacattg atgacttgtc cggtgatata tggattttgt gctaagaaaa
    20881 ctgctgtttg tgcaatatct tctacttgtc cgaaacgctg catcggaatt tgcttttccg
    20941 cttgttcctt gactttgtct gctaaaactt ccgtcatatc agttgtgatg aatcctggcg
    21001 caatggcatt acatgtaatg cctctagttg cagcttctcg tgccacagat tttgttaatc
    21061 ctactacgcc agctttacta gctgcataat ttgcttgacc aatatttcca atcaacccag
    21121 atacactaga gagattaatg atggcccctt ctcgctgctt catcattttt ttcaatacgt
    21181 gctgcgtcat attgaaggtt ccaatcaaat tgatatccag acattttttg aaatcttctg
    21241 catccattcg catcacaagt ttgtcatttg tgattccggc attgtttact aacacatgga
    21301 tcgatccaag cttttcttct gcttctttga tcatttgtcc cgctttctca taatcagaga
    21361 tgtcaccgga aactcctacg cattttacac cgaatgcttc gatttcttct atcttttctt
    21421 ttggaatctc tccgcgacca ttcaaaatga tatttgctcc agcttttgca aaagctaagg
    21481 ccatcgcttt gccgattcct cttgtactac ctgtgataaa tacatttttt ccttttacat
    21541 ccataacgtt cctccttata gtattgctat tgtttcatca aatgtctgct tattctcaac
    21601 acgcagaact ggtattgttt tatcgatttt tttcataaat ccgcttaata cttttccggg
    21661 gccgacttcg atgacttgtt cgatgccgat tgtttttaac ttatggatac tctcatagaa
    21721 acgaacaggt tgcatgactt gctgttcaag tagtcccgcg attgtttctt tcttcattat
    21781 ttccgttgtt gtgttgctta tgataggaaa agaaggttca gagaattgaa tctgacttag
    21841 gtccttcgcc agtttttttg ctgccggttc taaaatagct gtatgaaaag gcccgctgac
    21901 atttagaggg atcattcttt tgaacccttt ttcttttaat agtaaaacag cttcgtctac
    21961 tgctttttct tcgccaccaa tcacgatctg ctgaggtgta ttataatttg caggagatac
    22021 gatgccgtac ttgctagctt catgacagct ttcctcgatc gtttcaatcg gcgcattcat
    22081 cactgcaacc attttcccgc ttccagctgg ggcagcttct gtcatataag cgcctctttt
    22141 tgctactaac gcaactgctt cagtgaagga tagcgcatta ctagctacta atgccgaata
    22201 ttctcctaaa ctcagtccta aagctgcatc tgggatgatt ccatgctcct tcaataagcg
    22261 ataatacgcg atgctgactg ttagaatagc tggctgtgta tattgcgtct gatccaaacg
    22321 ttcattttct gtaaagcaaa gttcagccat atcgtagccg agaatctcac ttgcttcatc
    22381 aaatgtctgc tttacaacgg cttcttcata taaatctttt cccatacctt gatattgtgc
    22441 accttggcca ctaaataaga atgctgtttt catgtttttc tccaatatat ccagaatata
    22501 tgtttgattc ttcctattca aaaaggccga gacattccta tttcaggaaa tgtcccagcc
    22561 cccaacctaa ttttaatgat tatgcagttt gtttttctac gtaagtcact aaatcttgaa
    22621 ccgttttgat tccgtcttct gtttcgattt tcacgtcaaa ttcatcttca atttcattga
    22681 tgatttggaa taaatccaag ctgtcagctt ctaagtcttc ttggatgtca gtagtcaatt
    22741 tgatttcttc agtttctttt cctaattctt ctgcgatgat ttcttgaatt ttttcgaata
    22801 ccataataat atattcctcc gatattgttt gctttttatt tttataattt aacagtctac
    22861 aactccatga gaatacttcc ccatgttaaa cctccgccat agcctgtaaa tattactttc
    22921 tgctgttttc ctaaggtgat ctttccacta gaaactgctt catccaataa gatagggatg
    22981 cttgcagcag atgtattccc atatttatcc atattggtca ggaacttttc ttgcggcatc
    23041 tttacttttc gggcaatttt ctcaataatt ctgaggtttg cctgatgcaa aagcagataa
    23101 tcaactggtt tgtctttcat gacttcttgg atattgtttg caacgtctcg tacggcaaaa
    23161 tcaaaaatat ctctgccagt catttgcaaa taatacgaac cttctgaatc ttctgaatag
    23221 aacggatttt tattttgctg gtagccagcg gtcaatgagc tacttctggt accgtcagct
    23281 gctaattttt ctttcagcaa ttttttctgt ggattcgctt ctaagagaat cccacctgct
    23341 ccatcgccaa acaacacagc agtcgtacga tcagaccaat cgattagctt agatagtgtt
    23401 tcaccaccaa tcaccagtcc cttagttgag ccgcagcgga tcattttttc agccatgctt
    23461 aacgcataaa caaatcctga gcacgcagca ctgatatcaa aagcgatagc atgagttgct
    23521 cccaatttcc cttgtacttg tgctgctaca gatggtgtcg tgtaatcggg cgacattgtt
    23581 gctactaaaa taaaatccaa atctttaggg tttagatttt tctgttttat caatttttcc
    23641 gccactaaaa tgcataaatc agaagtctcc tgatttgtta cacatcttcg ttcacgaata
    23701 cccgtccttg acgaaatcca ttcatcagag gtatccatca tcttggaaag atcattatta
    23761 gtaataatct tttcaggaag gacgctggct gttgcagtaa tcattccaaa ttgttccatt
    23821 aaatatcctc acttatagtc ttgcaagaag tcgtgcaagt ttttcagtgc atccagcaag
    23881 gcatgctgtt cctccgtagc cattccgtct aagatgttct tgaccatttc acgatgaaaa
    23941 tgctgatgga cccgaaatag taactttcct tttttcgtta gtcccaactt tacaacgcgg
    24001 cggtcatctt cactgcggat acgttcgaca tagccttttt ttactaactt gttaatagca
    24061 gtggtcaagg tcccaacagt aattgatagt tctttggcta cttctgaagt tgtttttttc
    24121 ttgtacatcc cgattgcctc gatcgtgtgc atctctgtga ttgacagatc tttgaattga
    24181 gattttttca gctcagactc ttcgattgtc aaaatatcat tgaagacact aacaagataa
    24241 tcatttactg tttttaaatc tggttccatg ttccaccgtc cactaattag tttgattatc
    24301 aaatcttttg atgttcaaac tatatcttaa cttttttcaa aatgcaacct taaaagatca
    24361 tttttttact atatgagctt tttttaagat tttactcatt taaaacaact gataaatcag
    24421 catttataat ttcttttatt agaaaaatca tctatctcac atattttgat tatcaaagta
    24481 tttt
//
LOCUS       DACDKM010000027        24457 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20834_2016
            SAMN12640370-rid9891663.denovo.027, whole genome shotgun sequence.
ACCESSION   DACDKM010000027 DACDKM010000000
VERSION     DACDKM010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640370
            Sequence Read Archive: SRR10040891
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 24457)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24457)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742835.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742835.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:04:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,012
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 29 of 75
            Pseudo Genes (incomplete)         :: 33 of 75
            Pseudo Genes (internal stop)      :: 19 of 75
            Pseudo Genes (multiple problems)  :: 5 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24457
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640370-rid9891663.denovo.027"
                     /isolate="E20834_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            102..830
                     /locus_tag="IV334_001107"
     CDS             102..830
                     /locus_tag="IV334_001107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF554 domain-containing protein"
                     /protein_id="HAP8943194.1"
                     /translation="MPTGVLINVGSVLLGGLIGGLVGNKLSEHFKAQLTMVFGVCSMG
                     MGIYSIAPMKNMPAVIFALVIGTAIGLIVHLGNGINKGAALMQVPISKIFPSEKLGMT
                     HDEFISTLVTVIVLFCASGTGIYGSLDSGMTGDSTILISKSVLDFFTAAIFACNLGYV
                     VSVIAIPQFIIFYILFLLAKFIYPLTTPDMILDFKACGGFLMVATGFRMINVKMFPVA
                     DMIPAMIVIMPLSWMWTNWIMPLL"
     gene            986..1303
                     /locus_tag="IV334_001108"
     CDS             986..1303
                     /locus_tag="IV334_001108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HAP8943195.1"
                     /translation="MHIQIPTVLLDGTVLAALSKEDMYGYALTKIVQESLSVSESTLY
                     PVLRRLKKEGYLETYDEPFEGRNRRYYSLTPMGSRHLEEIIDEWIVFRDSLDRLLEES
                     TNE"
     gene            1296..1904
                     /locus_tag="IV334_001109"
     CDS             1296..1904
                     /locus_tag="IV334_001109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1700 domain-containing protein"
                     /protein_id="HAP8943196.1"
                     /translation="MNEYLNEVVDHLQEIPEDDRFDLIQYYEEYFLDSGKTLDQIIDE
                     YGTPKQFALKLKISYFSEEEQAENELVKNPKRQIRLIWLIIIGLFASPVLIPLALGFI
                     LVIGGLLVALLALIFSIYVLCISILGVGLFMIISGISVFGQSILSGLFFIGVGLFATG
                     SAVFISPLLLKGTKGLFYVMMNFVKWMGRRFITKRDLHSAGV"
     gene            1920..2750
                     /locus_tag="IV334_001110"
     CDS             1920..2750
                     /locus_tag="IV334_001110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 family beta strand repeat protein"
                     /protein_id="HAP8943197.1"
                     /translation="MRLKHFLITGIGLMLTGGLLAGATYTLGAQKSLTWDNGPRLIDM
                     VTETKKIKDANKIVIHAENQTVSVIRGLDFEIKTTYDKTEKPEIKEKNGVLTVNAVKK
                     STGAIISLEDTSAQITIVVPADHELSELTIEGNNSAIDINEVSIKKVNTKTHDGWVSF
                     NEFTGGTITAKDELGSLRLDEANVDNLIVTGENSNFHLEDSLISKEGKVILNGGNLSL
                     HEMENSGYTIQTSGESYIEKDYQPITNTLLEQGQKKLKITAKDTSINITTDQEDNETD
                     "
     gene            2888..3400
                     /locus_tag="IV334_001111"
     CDS             2888..3400
                     /locus_tag="IV334_001111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5348 domain-containing protein"
                     /protein_id="HAP8943198.1"
                     /translation="MKVEEGQREKLKTEMERLHTYITQLSQTFYDPDKEKVMVNYPNN
                     SEGRQLEQVYHEVFKHLLTVKKELDYYSLPIIDTGILKYDGKKERFIFKSVREDLPLS
                     AGMDLEVLVEDYFTEEKHWVRTKLDYLPQAAGGTQARGWYITEDKELELEGVMARIRK
                     KIKKVTLDIY"
     gene            3426..4298
                     /locus_tag="IV334_001112"
     CDS             3426..4298
                     /locus_tag="IV334_001112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HAP8943199.1"
                     /translation="MREKRAFYINGYAGIVGLVVLALVGLFFFYLGMWQAKVWALFLS
                     IFLWLITLLLLSSATVVSPNQAKVILFFGQYLGTIRENGFFLTIPLAQKMTVSLKVRN
                     FNSSVLKVNDLDGNPIEISAVVVFKVVDTAKALFDVAYYQDFVEIQSETAIRHIASQY
                     PYDTFNENDLTLRGNTTAVSDELQKELQERLAVAGVEVIETRLNHLAYATEIASAMLQ
                     RQQAKAILSARQTIVEGAVTITQMALEQIEDGQEINFTDDRKVQLINNLLVSIITDKG
                     TQPVINTGDMKDRK"
     gene            4474..4836
                     /locus_tag="IV334_001113"
     CDS             4474..4836
                     /locus_tag="IV334_001113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HAP8943200.1"
                     /translation="MKKLLIGLLVFIVVVLAGVKIADYVVMGGEEYYVQITTDGTKTV
                     SKSDSGQETVNYRYILPGYTKEGKEKELDFKGQLTRPLRKGAYLKITWNKNKGVTSYE
                     EVEKKDVPKTALEKLIKG"
     gene            4845..5597
                     /locus_tag="IV334_001114"
     CDS             4845..5597
                     /locus_tag="IV334_001114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP8943201.1"
                     /translation="MNKIVEVQNVTKVYGKNNEKKTQALSGISFDVEKGEFIGIMGAS
                     GSGKSTLLNILSTLDKPTDGHIRINQKDVTTLKGNQLADFRANEIGFIFQDFNLLESL
                     TAQENIAVPLSLQGVRPKEIKQRVQKIAERLSISHILESYPSEISGGQKQRVAAARAL
                     ITQPTILLGDEPTGALDSKSARDLLDTMDELNTKDHVSILLVTHDPFSASYCQRILFI
                     KDGGIHQEVKRGDQSRDSFYREILSILGNLEQ"
     gene            5611..7413
                     /locus_tag="IV334_001115"
     CDS             5611..7413
                     /locus_tag="IV334_001115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP8943202.1"
                     /translation="MLWKLSLTGIKSRLRDYLVLFSGLVMASAIFYMFESMASNEAFL
                     KSNTIVAATVIIFRFGSVLLGIITFVYILYANSFLMTMRQKDYAMFMMLGAKGRKIAQ
                     MIFSETFLVGIAATLVGSAVGVGLTSIVHRLLVDQLDLNISHFTPFSIHGLLITVLFF
                     AVLFLLAAVVNAFSIVKKPILALIRADQTPTRIKQHKFLFFLEVVLGIAFLGCGYYMM
                     KHVGTYQVFGIGVALVTIILGTYFIFHSVIIFFLSLLKKSEQISLKKLNNFTLSQLNF
                     RIREYTQMLSMVAMLFALALGALTVGLGFKNEIADLTENNSGYDLVMNNPRAEDQQKI
                     KELSPTLSASYTQKEDAGTIYYNASEFAAQPLIMIKHEDGTPPKITKEKVPAKKMNEL
                     TVQEELRSYELPEQQEKEIKVVSQSEFDQLNLPQSTLQLVQVKDFQANLKPIETLVAQ
                     NKMNNPSLQKVEYSNQKYEMYEVYNGLFSGFEFMGFFLGLAFLTMLASCLMFKILSGS
                     KSDIARYEMLEKIGTRRGLLKQSIRREVGVLFLAPGILGVVHVLFGLQMFGLFMQNPY
                     KDIWVPFTIFFVLYFIYYVLTTWLYTGIVLKKRL"
     gene            7690..8730
                     /locus_tag="IV334_001116"
     CDS             7690..8730
                     /locus_tag="IV334_001116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose mutarotase"
                     /protein_id="HAP8943203.1"
                     /translation="MEINEQNFGENYHLITITNQNGTSLSVSDLGARVVHFQTIIDNE
                     KRELILGFDEAKEYLEKDPYIGASIGRMAGRITNGTFKLDDETFEVKTDPDTGHCLHG
                     GAPSFEAKQWHYTVINGENEASVIFYQTSPDMGNGFPGTLDVEVRYTLTNDDTWRVTV
                     QGISDRKTVFNPTNHVYFNLTGDPAQSIDEHILWLNSRLFATLNKDTTPTGEVVSVSG
                     TAFDFQRPKKLGEVFASDFRQSMIVGGIDHPFLLKKQSGLLNAAELTSPDEKVSIQVK
                     TDASGIVIFTANFGEDGPEMRGNKLVNHGGITFETQELPGAERLESFGDVTLEPNQVR
                     EFVTEYKINIKK"
     gene            complement(8782..10926)
                     /locus_tag="IV334_001117"
     CDS             complement(8782..10926)
                     /locus_tag="IV334_001117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HAP8943204.1"
                     /translation="MKNKKTIRLLAATWLIAPSVLSTNTVFAVDNQIADQTVTTDTTT
                     SSSATSETTDSFTEDPTGTTNSTTGSTEPTEIDPADPSENEKPAETYSLTIAPSLIGK
                     IQLTASTADSPEQQPLDITESGTVEGLLAETKVTYTITPAEGEQIASVAVNGAKEVNY
                     DAKTFTVGTNNLSITAEFKAGPDTSTEKPTDPSEEVPSTPDPETPNKPDVGNNNNNNG
                     SNNSNNNNNNSNSGNSSNQTNPSGSNNNGNTQREPNHPNNGSSNGGTNNASSRPNTAN
                     QSGIENPSSASSDFVVKSPIDAVLPTNTTNVQQAIVREAFKHLGKPYVWGAKGPNTFD
                     CSGLTYYVYMKATGHYIGGWTGEQQYAGTQIPVSQAQPGDLVFWGPSSGVTHHVGIYI
                     GNGQFIHAPQPGDKVRVTSISDFTPDFAVRVNLAGLPAASGSLTGGSSILDGLDGSFH
                     FSQNQTTDQFLKKIADDAQEIGQKEGIYASVMMAQAILESGSGNSLLSSEPNHNLFGI
                     KGSYKGSSVTFNTLEQDSSGQSYQIRAQFRKYPSYKESLEDYADLIKNGLTGNPDFYK
                     PTWKSETKDYKEATKYLEGRYATDRQYSQKLNAIIEAYDLTKYDEPKKEAATTEEANA
                     EILNMSKRFDVPIKWTTDGVPITGLEFSTIKRVNNASSIFRVASIWDLWNNFTARQIP
                     ETTTRTVKAATVPVLTLLSLDKSFWLDLPLKK"
     gene            complement(11283..12656)
                     /locus_tag="IV334_001118"
     CDS             complement(11283..12656)
                     /locus_tag="IV334_001118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP8943205.1"
                     /translation="METTMNPEQMVLSEQIHMLIEQLGGKDNIDSVLPCATRLRIKLK
                     KQPVANAEKLKQQIPSIKYFILLDRYAEIIMDQGLAPAYKELTRQIDHSAPKEKLNPI
                     EKLFGLISEIFTPIIPLFAGSGILKGLVVLATTIGWLSETSGTYHILSAASNAVFYFL
                     PIFLAFSAAKTFKVNGYIAAVIAAALIEPNLTSLLSDSGKAATDFLGIPVVLMQYTST
                     VMPAILGIFFYSFVEKFLKKYIRENMQLVFVPLLSLVITVPLTLMVFGPAGVYLGEGL
                     AAAITWMMDINGPISGAIIAGGWNILVIFGIQWAVNPVMISNISAYGFDRIVPLTGAA
                     NFGMAGAALGVFLRSKRSKTRSISGSAFASILLAGVTEPTVYGIAIPLKKPFVAACIG
                     AAAGGAVMGFAQVKAIAFVFGSLTTLPAFISGTFFWYLAGLAVSLVVAMITTLVSGFD
                     EDLMSYE"
     gene            complement(12656..13051)
                     /gene="rbsD"
                     /locus_tag="IV334_001119"
     CDS             complement(12656..13051)
                     /gene="rbsD"
                     /locus_tag="IV334_001119"
                     /EC_number="5.4.99.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002829567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-ribose pyranase"
                     /protein_id="HAP8943206.1"
                     /translation="MKKGKVINSDISRVIAQMGHFDKLSIGDAGMPVPFGTEKIDLAV
                     SNGLPSFLSVLENVLEELEVQSIELAEEIKTMNPEILEKIKLLLPDTPISFIPHEEMK
                     KELNNCHAFIRTGEMTPYANIILVSGVVF"
     gene            complement(13079..13996)
                     /gene="rbsK"
                     /locus_tag="IV334_001120"
     CDS             complement(13079..13996)
                     /gene="rbsK"
                     /locus_tag="IV334_001120"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HAP8943207.1"
                     /translation="MNTVTVIGSINMDTTLRLTNMPKPGETMHAHEIFHAGGGKGANQ
                     AVAAKRSGARTSFIGGVGADSEGQQLLDLLTKENIDTSGIAEIQGATTGQAMIMVDAA
                     GENSILIHAGANNAFHEQEVLKNKQLITNSDFIIAQFESSLDATILAFSIAKDAGKTT
                     ILNPAPARETIPTELLEKTDIIIPNETETEIITGIRVTDHNSLVAAAEKLHELGIGTV
                     IITLGSAGAFYHTEKEHGIVPAFKVDAVDTTAAGDTFIGALSSTLQPDLSNLKEAILY
                     GNLASSVAVQSYGAQPSIPYREALEQSAV"
     gene            complement(14104..15117)
                     /locus_tag="IV334_001121"
     CDS             complement(14104..15117)
                     /locus_tag="IV334_001121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAP8943208.1"
                     /translation="MVKKKITIKEVAKLSGVSITTVSLILNGNTEKFSPKTVQKVLAA
                     KEELGYQPDYFARQMITKETKTIGVLVPDITNPFFSTLMRGIEDILYKQNFVTILCNA
                     DSDHQKEIEYLAELTRRGVDGFIIATSAVSTDAINENLKKQGRPFIVLDQKKSEGFSD
                     AVRTDDFRGGYLAGMHLLSLGHQTIALVYPENPPENVHARIEGFKSALDVYQIPHDQL
                     ILLPTQFSKQGGYQITAELLDSAATGVFALNDELAFGLYRGLEEAGKSIPEDYSIIGY
                     DNIDMCEYIKPKLTTIAQPIFELGQTSAKLLLDRIQFPEKEWEEKRLPVRLEKRFSTA
                     PLK"
     gene            complement(15198..15797)
                     /locus_tag="IV334_001122"
     CDS             complement(15198..15797)
                     /locus_tag="IV334_001122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HAP8943209.1"
                     /translation="MNDLKENMIFDLSKTAFVAIDMQKEIVNNGTLSPHTAASILTNN
                     DLLVRTLKRTAALKVLVNVDISTFPYLSQQTDMGGMTASVPPEFTDLLLKDSLKDTDN
                     MLTIKKYNPSAFFGTSLDLQLRRRGIETIILSGVATTNGVYATALDAFQHGYHIVLAE
                     DACSDRDKESHQLFIKKIFPKTARVRSTKQIIEAIQQSK"
     gene            15983..16684
                     /locus_tag="IV334_001123"
     CDS             15983..16684
                     /locus_tag="IV334_001123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP8943210.1"
                     /translation="MKYVEVKNEYKRYKMGETTITANDDISFSIEKGELVIILGPSGA
                     GKSTILNILGGMDTPDEGQIIIDDTDIAQFSDKQLTAYRRTDVGFVFQFYNLVPNLTA
                     KENVELATEVSPDALDPVEVLRQVGLAHRLNNFPSQLSGGEQQRVSIARALAKNPKLL
                     LCDEPTGALDFETGKQVLKLLQNASRQHGNTVLIITHNSALAPIADRVIHINDAKVRS
                     VELNDHPSSIDEIVW"
     gene            16694..19405
                     /locus_tag="IV334_001124"
     CDS             16694..19405
                     /locus_tag="IV334_001124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HAP8943211.1"
                     /translation="MKTRTYLKAGFREIRQSKGRFIAIILIVMLGTLLFVGVKTAGPV
                     MQKTMDHYVGTAGLSDLQIVSTGGLTQKDIAQAEKIPDAKVETGKQIYYSNTGKNEVI
                     QIFSYNKNNKQNKLQLTDGKLPEKPNQLVLDEKAEDEGYKIGDIYQIDSDELKEKEYT
                     ITGFVRSPLFINNLERGYANVGNGSVDYFVYLPESVFKTDIYSVLYLDFTNVKNLNTY
                     SKAYKDKMEQNQEKAEKYLQDRPEERLEELKKSANEELEPAQQKIADGKAQTTQARTR
                     LEDAKKQLAQQSATIQQLPEAQRTIAVQTLPQQEAQLAEQERQLVEKERELASAEEEL
                     NRNAEKIENLTKPSYLYNERSENVGFQEFGDLSDRIAAIANVFPVFFFFIAALITFTT
                     MTRMVEENRREIGTLKALGYTKVEIAKKYAIYASLASGIGIVLGTILGTNLLPRIIFE
                     LSNERYDIGSALIFYDWPPILQAAAAFFIAAFGAAMIVLFKDLRERPAALLQPKAPKP
                     GKRIFLEYITPLWSRLSFNQKISYRNLFRYKSRMLMAIIGIAGCAGLMVAGVGLKDSL
                     SSVSAKQFGPIIDYQAIVTLDSSTEDSRQKAADMLQKQEKVNEILAIHTQTVELRKKG
                     HASQSLTVIVPSKTADLDRFIHLTDLEENPRSLPKTGIAITQKVAELFDLSTGDHISL
                     YDEDQKEWKVEVKEIVKNYLGSFIYMSPEYYASVTDQSMMDNAFLLRTDKMTTKEEEM
                     LSEKLLATDAVTNTSFMSKQIETQEESMANLDSVVLIFVVLSGLLAFIVLYNLTNINI
                     SERVRELSTIKVLGFFDKEVTMYIVRENIIFTLLGIIGGFGVGYILTDFILQQASMEN
                     VIFPLVITWWAYVLSAGLTIVFTVIVMIVTHFKLKHIDMIDALKSNE"
     gene            19802..21430
                     /gene="treC"
                     /locus_tag="IV334_001125"
     CDS             19802..21430
                     /gene="treC"
                     /locus_tag="IV334_001125"
                     /EC_number="3.2.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-phosphotrehalase"
                     /protein_id="HAP8943212.1"
                     /translation="MTFKDKVIYQIYPKSYKDSNGDGIGDLKGIKEKLPYLNDLGIDM
                     IWLNPIYPSPQKDNGYDISDYTAIDPIFGTMEEFEQLIAEAKKYRIEIMLDMVLNHVS
                     TEHEWFKKALAGDQHYQDFFILREEPTDWVSKFGGSAWSPFGDTGKYYLHLYDRTQAD
                     LNWRNQAVREELFKVVRFWMEKGVKGFRFDVINVIGKDEQLKNNKENDGKPEYTDKPI
                     THTYLKELNQATFGTDSTIITVGEMSSTTIENCILYTEPSREELSMVFNFHHLKVDYE
                     NGNKWSTPPFDFAELKRLFHTWGENMSEGNGWNALFFNNHDQPRALNRFVVIDQYRKQ
                     GAEMLATMIHLNRGTPYIYMGEEIGMIDPDYSSISDYIDVESLNAYKLLLEQGFTEKE
                     AFRRIKAKSRDNSRTPMQWSDTEQAGFTTGKPWLKVGGKLEEINVEKELSSEDSILSY
                     YKKLIRLRKTYPIVAQGDYHAYEANHPEVYGFLRQFEGQQLLVLTNFYDKDTRIAIPE
                     GFTEANILIDNYSDTVLHQSLELKPYQAIALLKN"
     gene            complement(21490..22671)
                     /locus_tag="IV334_001126"
     CDS             complement(21490..22671)
                     /locus_tag="IV334_001126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HAP8943213.1"
                     /translation="MKKETDTTNRLIRFLGGKTSYYVLGIVILLALIIYFFHQISFIF
                     KPVGIIIATILPPLLFALILYYLLEPVIKRLSKHLSRNLSVIIVYVIILFLLGLGSIW
                     LIPFLEEQAKTLISSLPDLFQDFQESLRQFLEKTPFADSFNQFFASIDDVTSDIAGFI
                     GNYWESGAQRISNIFSTVTAIFITLFTGPIVAFFLLRDPQKFYQSVLAIVPPAFRTDF
                     KNLTKIANQQLGSFLKGQIIASFILGAVYWVCFLLIGLEFASVLAISAGLLCIIPYIG
                     PFIAFFPGLIIAFQDSTFMAVKFVIVWFAVQLLHGDLVIPRVMGDRLQIHPITILIVL
                     LVMGDLMGIVGVIFGIPIYTLVKLLVTFIFRKFKQRYNKFYGDKGEYENSSFSEEDYF
                     K"
     gene            22923..24446
                     /locus_tag="IV334_001127"
     CDS             22923..24446
                     /locus_tag="IV334_001127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     AdcA"
                     /protein_id="HAP8943214.1"
                     /translation="MKRLIGILAMLVLAGMLTACGASGKAEDSKEKLSVMTTFYPMYD
                     FTKAIVGDEGEVELLIPAGTDSHDYEPSAKDMAKIQDTDIFVYNDENMETWVPAIQKT
                     LQEGNVHTIKATEGMLLLPGSEEGHDHDHEHGEEGHTHELDPHVWLAPSLAIKQVANI
                     RDQLIEAYPEKQEVWTKNAAAYTEKLQALHQLYQETFKQAKQRSFVTQHAAFNYLALE
                     YGLNQVSIAGLSSSEEPSAARIAELKHFVKEHGINYIYFEENAKDSIARTLANEAGVS
                     LEVLNPLEGLTNEQIENGENYLSIMEANLEALKKTTETENPLEDTLTPKKEKSVYNGY
                     FEDEDVQDRSLSDWAGTWQTVDTYVEDGTFDPVFEYKEKLNQDKTAQEYKEYYTKGYQ
                     TDIASIKISEKEMAFTFKDGTTKRSNYRYVGKEILTYEAGNRGVRYLFEAEDKESGAF
                     RYIQFSDHTIAPADSEHFHLYAGNESQEVLLKEMENWPTFYPDTMDGREIAQEMLLH"
BASE COUNT         7917 a         4342 c         5003 g         7195 t
ORIGIN      
        1 taatcttctc tgtctgttac aatctttctt gcagcgaaag caataattac agaaattttt
       61 ctgatttttt tcgcttcgta gaaaaagaga ggagtttttt gatgccaact ggagttttga
      121 tcaatgtagg ttccgttttg cttggcggac taattggcgg attagtagga aataaattaa
      181 gtgaacattt taaagcacag ctgacgatgg tttttggtgt gtgttcgatg gggatgggaa
      241 tctattccat tgcgccgatg aaaaatatgc cggctgttat ttttgcttta gtgataggaa
      301 cagcgattgg tctgattgtt catttaggta atgggataaa taagggggct gcgctgatgc
      361 aagtacctat ttctaaaatc ttccctagtg aaaagcttgg tatgacccac gatgaattta
      421 tcagcacatt agtgacggtc attgttctat tttgtgcaag tggtacgggg atctatggaa
      481 gtttagattc tgggatgaca ggagacagta cgattttgat ttctaaatct gtccttgatt
      541 ttttcacagc agctattttt gcgtgtaatt tagggtatgt cgtttctgtg atagcaattc
      601 cccaattcat tattttttat attttgttct tattggcaaa atttatttat ccattgacta
      661 caccagacat gatcttagat tttaaagctt gcggcggatt cttgatggtc gctaccggtt
      721 ttagaatgat caacgtgaaa atgttcccag tagctgacat gatacctgca atgatcgtta
      781 tcatgccact gagctggatg tggacaaatt ggattatgcc actgttataa taaaaatcaa
      841 agagaagcca ttttttgctg actaaaaagg atcaaatctt ttagtcagtt tttttgtatt
      901 tgacaaactg attttgaaac tatattatat gttgtataat atagattgaa ataaaatatt
      961 acacttgaga atagaaggtg acgtaatgca catccagatt ccaactgttc ttcttgatgg
     1021 tactgtgcta gcagcattaa gcaaggaaga tatgtatggg tatgccttaa caaaaatcgt
     1081 acaagaaagt ctctctgtca gtgaatctac actatatccg gttttgagaa gattgaaaaa
     1141 agaaggatat cttgagacct atgatgaacc ttttgaaggc agaaaccgtc gatattactc
     1201 gttgacacca atgggaagtc gtcatttaga agaaattatt gatgaatgga ttgtttttcg
     1261 agattcttta gaccggttat tggaggagag cacaaatgaa tgaatatttg aatgaagttg
     1321 tggatcatct tcaagaaatt cctgaagacg atcgttttga tttgatccag tattatgagg
     1381 aatatttttt agattctgga aaaacattag atcagatcat tgatgaatat ggtacaccaa
     1441 agcagtttgc cttaaaatta aaaatcagtt acttttcaga agaagaacaa gcagaaaacg
     1501 agttagtgaa aaatccaaaa agacaaattc gcctgatttg gttaatcatt atcggtttat
     1561 ttgcttctcc tgtcctcatt ccactagcac tggggtttat attggttatt gggggactat
     1621 tggttgcttt actagcgctc atttttagta tctatgtttt atgtatcagt atcttaggtg
     1681 ttggcctatt catgatcatc tcaggaatca gtgtgtttgg acaatcgatt ctgagtggat
     1741 tattctttat cggtgtcggt ctgttcgcca caggaagcgc agtatttatt tcgccactgt
     1801 tgctgaaagg aacaaaaggg cttttttatg tcatgatgaa ctttgttaaa tggatgggac
     1861 gacgttttat tacaaaaaga gatctgcatt cagcgggcgt ttgaaggagg acacataaat
     1921 tgagattgaa acattttttg attaccggta ttggtctgat gcttactggt ggcctcctag
     1981 caggtgcaac ttatacatta ggcgctcaaa agagtctgac ttgggataat ggtcctcggt
     2041 tgatcgatat ggttactgag acaaaaaaaa tcaaggatgc aaacaaaatc gtcatccatg
     2101 cagaaaatca aacagtttcg gtcatacgcg gattggattt tgagataaaa acgacttatg
     2161 ataagacaga aaagccagaa ataaaggaga aaaatggcgt gttgactgtg aatgcagtca
     2221 aaaaatcaac aggtgcaatc attagtttag aagacacaag tgcacaaatc actattgttg
     2281 tccctgcaga tcatgaatta tctgaattga caatcgaagg gaataatagt gcaattgata
     2341 tcaacgaagt atcaatcaaa aaagtgaata ctaagacaca tgatggctgg gtatcgttca
     2401 atgaatttac aggaggaaca ataactgcga aagatgaatt aggctcactt cgattagatg
     2461 aggccaatgt ggataacttg atcgtcacag gtgaaaacag caacttccat ctagaagaca
     2521 gtttgatcag caaagaagga aaagtcatcc taaacggtgg caatttatct ttgcatgaga
     2581 tggaaaacag tggctatact atccaaacgt ctggtgaatc ttatattgaa aaagactacc
     2641 agccaataac aaatactttg ttagaacaag gacagaaaaa gctaaagatc actgctaaag
     2701 acacatcgat caatattacg acggatcaag aggacaatga aacagattga tcctgtactc
     2761 aactggatat tttaagaaaa aacagcaaag atccttccaa tctttgctgt ttttcatcgc
     2821 ttaacgcaca taactatagt aaaataagaa agaaaatatc taaaaagaaa acagggtgaa
     2881 agagtatatg aaggtagaag aaggtcaaag agaaaagtta aaaacagaaa tggaaagatt
     2941 acatacttat atcactcaac taagccaaac cttctatgat ccagacaaag aaaaagtgat
     3001 ggttaattac ccgaacaatt cagaaggtcg ccaattagag caagtgtacc atgaagtttt
     3061 caaacattta ttgacggtga agaaagagct ggattattat tctttaccga ttatcgatac
     3121 aggaatattg aagtatgatg ggaagaagga acgatttatc tttaaatctg ttcgggaaga
     3181 tttgcctctt tcagcaggga tggatctaga ggtcttggta gaagactatt ttacagaaga
     3241 aaagcactgg gttcgaacaa aattggatta tttaccgcag gcagccggag ggacacaggc
     3301 aaggggttgg tacatcactg aagacaaaga gttagaacta gaaggtgtaa tggcgcgtat
     3361 tcgaaaaaaa attaaaaaag taacattaga tatttattga aggataaagg agcgcaggaa
     3421 gtagcatgag agaaaaaaga gcattttata tcaatggtta tgcagggatt gtcggattgg
     3481 tcgtattagc tttagttgga ttgtttttct tctatttagg tatgtggcaa gcgaaagttt
     3541 gggcactttt tttaagtatt tttctttggt tgatcacgtt attgttgtta agttcggcaa
     3601 ctgtagtcag tcctaatcaa gcaaaagtaa ttttgttttt tggacaatat ttaggtacga
     3661 ttcgagaaaa tggctttttc cttacgatac ctttggcaca aaaaatgact gtttcgttga
     3721 aagttcggaa tttcaatagt tctgttttaa aagtcaatga tttggatgga aaccccatcg
     3781 aaatctcagc agtagtagtc tttaaagtcg ttgatacagc aaaggcatta ttcgatgtag
     3841 cgtattatca agattttgta gaaatccaaa gtgagacagc cattcggcat atcgctagcc
     3901 aatacccgta tgatacattc aatgagaatg atttgacgct tcgaggaaac acgacggcag
     3961 tgtctgacga gttgcaaaaa gagctgcaag agcgtttagc agtagcaggt gtcgaagtaa
     4021 tcgaaacacg cttgaaccat ttagcctatg caacagaaat cgctagtgcc atgctccaac
     4081 gtcaacaagc aaaagccatt ttgtctgctc gacaaaccat tgttgaagga gctgtcacaa
     4141 tcactcaaat ggccttagag caaatcgaag atggacaaga aatcaatttc accgatgatc
     4201 ggaaagtcca attgatcaat aatttacttg tctcaatcat tacagacaaa ggaacgcaac
     4261 ctgttatcaa tacaggcgac atgaaagacc gtaagtgaca tactttttgt aagataggaa
     4321 gaaggagttt aggacggatt attgtcttga actctttttt tagttgtttt aaagaaaatg
     4381 acaaaattga aagaagaatg atagttgatt cgaaggcaga attgttcgct tttttctata
     4441 atcagtatat aaagtaagag agaaggggaa aaagtgaaaa aacttctgat cggtctactt
     4501 gttttcatcg ttgtggtact tgcaggggtg aaaatcgcag attacgtagt tatgggtgga
     4561 gaagaatact atgtacaaat tacgacagat ggtacaaaaa cagtttcaaa atcagactct
     4621 ggacaagaaa cagtcaacta tagatatatc ctgcctggct atacgaaaga gggtaaggaa
     4681 aaagaactgg acttcaaggg acaattgact cgaccgcttc gtaaaggagc atatttaaaa
     4741 atcacctgga acaaaaataa aggtgtcact tcgtatgaag aagtcgaaaa aaaagacgtg
     4801 ccgaaaacag cactggaaaa actaattaaa gggtagggaa agaaatgaac aaaatagtcg
     4861 aagtacagaa tgtaacaaaa gtatacggaa aaaacaatga gaaaaagacg caagcattaa
     4921 gcggcatcag cttcgatgta gaaaaaggcg aatttattgg gatcatgggc gcttctggtt
     4981 ctggtaagtc gacacttttg aatattttat ccacattaga taaaccaaca gatgggcata
     5041 tccgaatcaa tcaaaaagat gtgaccacat taaaaggaaa tcaactagca gattttcgtg
     5101 cgaatgagat tggctttatt ttccaagatt tcaatttatt agaaagcttg acagcacaag
     5161 aaaatatcgc tgttcctctt tctttacaag gcgtcagacc aaaagaaatc aagcaacgtg
     5221 tccaaaaaat tgcagaacgt ttatccatct ctcatatttt ggaaagctat ccttctgaga
     5281 tttccggtgg acaaaaacaa cgggtcgccg ccgcacgtgc attgatcaca caaccaacga
     5341 ttctattagg ggatgagcct acaggtgcgc tggattccaa aagtgcccgg gatcttttag
     5401 atacgatgga cgaattgaat acaaaagatc atgtatcgat tcttcttgtg acgcatgatc
     5461 cgttttctgc aagttattgc caacgtattt tattcatcaa ggatggcggt atccatcaag
     5521 aagtgaaacg tggagatcaa agccgtgatt ccttctatcg agaaatacta tctattcttg
     5581 gaaacttgga acagtagggg gagattgact atgctttgga aattatcact tactgggatc
     5641 aagagccgtt taagagacta cttggtgtta ttctcaggat tagttatggc atctgctatt
     5701 ttttacatgt ttgaatcaat ggcaagcaac gaagcttttt tgaaaagtaa tacgatcgtt
     5761 gcggctacag ttatcatttt ccgattcggt tctgttttac tagggatcat cacattcgtt
     5821 tatattttat atgcaaattc ctttttgatg acgatgcggc aaaaagacta tgcaatgttt
     5881 atgatgctgg gagcaaaagg cagaaaaatc gcacagatga ttttttctga aacattctta
     5941 gtagggatcg cagctacttt agtgggttca gctgtcggtg taggattgac aagtatcgtc
     6001 caccgattat tagtggatca gttagatctc aatatttcgc attttacacc ttttagtatt
     6061 cacgggttgt tgatcactgt tttgtttttt gctgttttgt ttttattagc tgctgttgtc
     6121 aatgcttttt cgattgtcaa aaaaccgatt ttagcattga ttcgtgcgga tcagacacca
     6181 acaagaatca agcagcataa attcttgttt ttccttgaag tagtactggg aattgctttt
     6241 ctaggctgcg gttactatat gatgaaacac gtggggacct atcaagtctt tggtataggg
     6301 gtagccttag ttacgatcat attaggaact tatttcattt tccattctgt gatcattttc
     6361 tttttatcat tactaaagaa atcagaacag atttcattga aaaaattgaa caatttcaca
     6421 ttgtcacagt taaactttcg tattcgtgaa tatacacaaa tgctctcaat ggtcgctatg
     6481 ctgtttgcct tggcactggg cgctttgacg gtaggattag gttttaaaaa tgaaattgca
     6541 gacttgacag aaaacaattc tggatatgat ctagtgatga ataatcctcg ggcagaagat
     6601 cagcaaaaaa tcaaagaact ttcaccaaca ctttctgctt cttatactca aaaagaagat
     6661 gcgggaacga tttactataa tgcaagtgaa tttgcggctc aaccattgat catgataaaa
     6721 catgaagatg gaacaccgcc gaagatcacg aaagaaaaag ttcctgctaa aaaaatgaat
     6781 gaactgactg tacaagagga acttcgaagc tatgaattgc cggaacagca agaaaaagaa
     6841 atcaaggtag tcagtcaatc agaatttgat caactgaact tgccacaatc cactttacag
     6901 ctagttcagg taaaagactt ccaagcaaac ctgaaaccaa tcgaaacact tgttgctcaa
     6961 aataaaatga acaatccatc tttgcaaaaa gtagagtaca gcaaccagaa atatgaaatg
     7021 tatgaagtgt ataatggtct attctctggt tttgaattta tgggattctt cctaggttta
     7081 gctttcttga ccatgcttgc tagctgtttg atgttcaaga tcttgtcagg aagcaagagt
     7141 gacattgctc gttatgaaat gcttgaaaag ataggaacac gacgcgggtt gcttaaacaa
     7201 tcgatccgta gagaagtagg cgtgttattc ttagcaccag gaatcttagg tgtagtccat
     7261 gtcttattcg gattacaaat gtttggtcta ttcatgcaaa atccatacaa agatatttgg
     7321 gttccgttta cgatcttctt cgtattgtat ttcatctact atgtactgac gacatggtta
     7381 tatactggaa tcgtattgaa aaagagattg tgaaaaagct gtcctgcatc aagtaataag
     7441 aacagccaag caaaaatagc tcctcatatt tttcgtttgg ttgggcttat taccgcagat
     7501 gcaagctttt gaacaccgtt tgttcggaaa aagaggttgt gacaatattt ttgtcacaac
     7561 ctttttttca cggtctttta ttagaatata gtaaattttt tctcgttact cacggaataa
     7621 gctgttaaga aaacggtttc catgtatact ggaatcagtg caaatgaaag aaaagagggt
     7681 gcaaggaaca tggaaatcaa tgaacaaaat tttggcgaaa actatcactt gattacgatc
     7741 acgaatcaaa atgggacatc tttgtctgtc agtgatcttg gggcacgggt tgttcatttt
     7801 caaacaataa tcgataatga aaaacgagag ttgatcttag gttttgatga agcaaaagaa
     7861 tatctagaaa aagaccctta tatcggtgct tcaatcggac gtatggctgg acggattacg
     7921 aatggcacat tcaaattaga tgatgaaacg ttcgaagtaa aaacagaccc ggacactggt
     7981 cattgtttac atggtggtgc acctagcttt gaagccaagc agtggcatta tacagtcatc
     8041 aatggtgaaa acgaagcatc ggtcattttc tatcagacta gcccagatat gggaaacgga
     8101 tttcctggaa ctttagatgt agaagtacgg tatacgctga cgaacgatga tacatggcga
     8161 gtgacggtcc aagggatcag tgaccgcaaa acagtattca atcctacaaa tcatgtctat
     8221 ttcaatttaa ccggtgatcc agcacagtca atcgacgaac atatactttg gttgaatagc
     8281 cgattatttg ctacgctcaa taaagataca acgcctacag gagaagtagt ttcagtaagt
     8341 ggcacagcat ttgacttcca acgtcctaaa aaattaggtg aggtctttgc cagtgatttt
     8401 cgccaatcaa tgatagtggg cggcatcgat catccatttt tgttgaaaaa acaatctggt
     8461 ttactaaatg ctgcggaatt gacaagccca gatgaaaaag tatcgatcca agtaaaaaca
     8521 gatgcttcag gaatcgtgat ttttacagca aattttggag aagatggacc ggaaatgcgc
     8581 gggaataaat tagtaaatca tggcggtatc acatttgaga ctcaagaact gccaggagcg
     8641 gagcgacttg aaagttttgg agatgtcact ctggaaccta accaagtacg cgagtttgta
     8701 acggaataca aaataaatat aaagaaatag aataaattaa aaagggagtg tgacagtgtc
     8761 acatcccctt tttaatttat tctatttctt taaaggcaga tcaagccaga atgatttgtc
     8821 taaacttaac aaggttagta caggaacagt tgcagcttta actgtacgag tagttgtttc
     8881 tggtatctgt cttgccgtaa aattgttcca caaatcccag attgaagcga cacggaagat
     8941 ggaagaagcg ttgttgacgc gttttatcgt tgaaaattct aatcctgtga ttggcacgcc
     9001 atctgttgtc catttgattg gcacatcgaa tcgcttagac atattgagga tttcagcgtt
     9061 tgcttcttca gttgttgctg cttctttttt cggttcatca tattttgtca ggtcgtacgc
     9121 ttcgatgatc gcgttcaatt tttgtgaata ttggcgatct gtagcataac gtccttccaa
     9181 atatttggtt gcttctttgt agtcttttgt ttccgacttc caagttggtt tgtagaaatc
     9241 aggattgcca gtcagtccat ttttgatcaa gtcggcatag tcttccaaac tttccttgta
     9301 agaaggatat ttacggaatt gtgcgcggat ttggtagctt tgtccggaac tatcttgttc
     9361 aagtgtattg aaggtcacgc tgcttccttt atatgatcct ttgataccaa aaaggttatg
     9421 atttggttcg cttgataata agctgtttcc agatccactt tcaaggatag cttgagccat
     9481 catgactgat gcgtagattc cttctttttg cccgatttcc tgtgcatcat ctgcaatctt
     9541 tttcaagaac tgatcagttg tttggttttg actgaaatgg aacgagccat ctaatccatc
     9601 taaaatagat gagccgcctg tcagcgaacc tgaagcagca ggtaaacctg ctaagtttac
     9661 tcggacagca aaatcaggcg tgaaatctga aatagaagtt actcgaactt tatctcctgg
     9721 ttgaggcgca tggataaatt gtccgttccc aatatagata cctacatggt gagtaacacc
     9781 ggaagaaggt ccccagaaga cgagatcgcc aggttgggcc tgacttactg gaatctgcgt
     9841 acctgcatat tgctgttcgc ctgtccagcc accgatataa tgaccggttg ctttcatata
     9901 tacgtaataa gtcaaaccag aacagtcaaa agtatttgga ccttttgctc cccatacata
     9961 tggttttcca agatgcttga atgcttctcg aacgattgct tgttggacat tcgttgtatt
    10021 tgtaggcaat acagcatcaa ttggtgattt tacaacaaaa tcagaagaag cagaagatgg
    10081 attctctata ccgctttgat ttgctgtatt tggacgagat gaagcattgt tcgttccgcc
    10141 attcgatgaa ccattgtttg gatggtttgg ttctctctga gtatttccat tgttattcga
    10201 tccagatggg tttgtttgat tcgaagaatt cccgctgttc gaattattgt tattattgtt
    10261 tgaattattt gaaccattat tattgttgtt attacccaca tctggtttgt ttggcgtttc
    10321 cggatctggt gtactaggga cttcttcact aggatcagtt ggtttctctg ttgaagtatc
    10381 cggtccagct ttaaattctg cagtaataga taaattgttc gtacctactg taaaggtctt
    10441 tgcatcataa tttacttctt tggcaccatt aacagcgact gaagcaattt gttcaccttc
    10501 agctggcgta atagtgtaag ttaccttcgt ttccgctaat aaaccttcta ccgttccact
    10561 ttccgtaata tccaaaggtt gctgttcagg ggaatccgct gtcgaagcag tcagttggat
    10621 cttaccaatc aaagaaggag caattgtaag ggaatatgtc tcggctggct tttcattttc
    10681 tgatggatca gccggatcaa tttcagttgg ctcggtcgat cctgttgttg agttagttgt
    10741 tccagttggg tcttctgtaa aagaatccgt tgtttcacta gtagctgagc tggatgtagt
    10801 cgtatctgtt gtgactgttt gatcagctat ttgattatct acggcaaata cggtattggt
    10861 cgacaaaaca gacggagcaa tcagccaagt agcagctagc aaacgtatcg tttttttatt
    10921 tttcataata aactagtcct tttcctttca ttgattttct gtcttattca gcattcatta
    10981 gacatttttt ggaacagata atcgtgtcga aaagcttttt cttttcataa atctgtctgt
    11041 gtattctaaa taaaacaatt tgtaaataag caacgagcat tcattcgttt ttctttgacg
    11101 ttcattttat tgcatagacc acctttgttt ggggataaac aagtggggat aaaaatggtt
    11161 atgcatatgt aaattatacc aaagtttgga aagtatcaca aaaatttata aaaataatga
    11221 tcctaaaaag aaaaagactg aaatatgtga taaacatctc atacttcagt ctttttcatt
    11281 aactattcat aagacattag gtcttcatca aacccgctaa cgagagtagt gatcattgct
    11341 acgactagac taacagccag tcctgctaag taccagaaaa aagtaccgga aatgaatgca
    11401 ggcaatgtgg tcagactacc aaatacaaaa gcgatcgctt tcacttgggc aaatcccatt
    11461 acagctccac cagcagctgc accaatgcaa gcagcaacaa atggtttttt caatgggatc
    11521 gcaattccgt aaacagttgg ttcggtcact cccgcaagta aaatcgaagc aaaagcagaa
    11581 ccggagatcg aacgggtttt tgaacgttta cttcgcagaa atacacctaa agctgcacca
    11641 gccattccaa aattggctgc tcctgtcaac ggaacgattc ggtcgaaccc ataagcagag
    11701 atattactga tcatgacagg gtttacagcc cattggatac caaatattac caaaatattc
    11761 caaccaccag cgatgattgc gccactgatc ggtccgttaa tatccatcat ccaagtaatt
    11821 gcggcagcta aaccttcgcc aaggtaaaca cccgcaggcc cgaataccat taacgtcaaa
    11881 ggaaccgtga taactaatga aagtaaaggt acaaatacta attgcatatt ttcgcgaata
    11941 tattttttga ggaatttttc aacaaaagaa taaaagaaaa tacctaaaat cgccggcatg
    12001 acagtagacg tatactgcat cagtactaca ggaatcccca gaaaatcagt agctgctttg
    12061 ccactgtctg aaagtaaaga ggtcagattc ggttcaatca gtgctgcagc aatgacagcc
    12121 gcaatatatc cattcacttt aaacgttttg gcagcagaaa aagcaagaaa gattggcaga
    12181 aaataaaaga cggcattcga cgcagcagac aaaatatgat aagttccgct tgtttcacta
    12241 agccagccaa tcgtagtcgc taaaacaacc agacctttta atataccaga accggcaaac
    12301 agcggaatga tcggcgtaaa gatttccgat atcaaaccaa atagtttttc gatcggattt
    12361 agcttttctt tcggagctga atgatcgatt tgacgcgtca attccttgta agcaggagcc
    12421 aatccctgat ccatgattat ttctgcatat ctatcgagta aaatgaaata cttaatggaa
    12481 gggatttgct gttttaattt ttctgcattg gctactggct gttttttcaa tttgatacga
    12541 agccgagtcg cacatggaag gacagaatca atattgtctt ttcctcccaa ctgttcaatc
    12601 agcatatgaa tctgctcgga cagtaccatc tgttcaggat tcattgtcgt ttccattaga
    12661 agaccactcc actcactaat ataatattgg cgtaaggtgt catttcgcct gtacgaataa
    12721 atgcatgaca gttattcaac tcttttttca tttcttcatg tgggataaag ctaatcggtg
    12781 tatctggtaa caataatttg attttttcca atatttccgg gttcattgtc tttatttctt
    12841 ccgccaattc aatcgattgt acctcaagtt cttctaaaac gttttctaaa acagataaaa
    12901 aacttggaag accgtttgag acagccaaat ctattttttc agtaccaaag gggacaggca
    12961 tgcctgcatc cccgatactt aatttatcaa agtgccccat ttgtgcgatt actcgtgaaa
    13021 tatctgaatt gattactttt ccttttttca tgtttgtttc ttcctctcat tgttaatttt
    13081 agactgctga ttgttctaaa gcttcgcggt aagggataga aggttgcgca ccataagact
    13141 gcacagcaac agaagaggct agatttccat aaaggatcgc ttctttcaag ttactaaggt
    13201 ctggctgaag tgtgctgctt aatgcgccaa taaatgtatc acctgcggct gttgtgtcaa
    13261 cggcatccac tttaaaagca gggacgatac cgtgttcttt ttctgtatga tagaaagcgc
    13321 cggcactgcc tagtgtaatg attactgttc caatacctaa ttcatggagt ttttcggctg
    13381 ctgcgaccaa actgttatga tctgtcactc tgattcctgt aatgatttct gtttctgttt
    13441 catttggtat aatgatatct gtcttttcaa gtagttcagt aggaatcgtt tcacgagctg
    13501 gtgcaggatt caggatcgtt gtttttcctg cgtcttttgc aatagaaaaa gctaggatcg
    13561 tcgcatctaa gctactttca aactgagcaa taataaagtc actgtttgta atcagttgct
    13621 tgttttttag gacttcttgt tcatggaaag cattatttgc ccctgcatgg atcaagatac
    13681 tattttctcc tgctgcgtct accataatca ttgcttgtcc ggttgtggcg ccttggattt
    13741 cagctatccc agatgtatcg atgttttctt ttgtcagtag atctaataac tgttgtcctt
    13801 cgctgtcagc cccaacgcct ccaataaaag atgtccgtgc gcctgaacgt ttggctgcca
    13861 ctgcttggtt ggcaccttta cctccgccag cgtgaaaaat ctcatgcgca tgcatcgttt
    13921 cgcctggctt gggcatgttg gttaaacgaa gggtagtgtc catattaata cttccaatta
    13981 ctgtaaccgt gttcatttta caaacctcca actatctcat ttattttgat gggaaaatta
    14041 ttttttaaaa cgttttagta aaacgtttta atagttcgat aaaaatatag cacaccttga
    14101 aagctatttc aagggtgcag ttgaaaatct tttttctaat cgtacaggta atcttttttc
    14161 ttcccattct ttttctggga attggatccg atctaacaat aacttggcag atgtttgtcc
    14221 caattcgaag attggttgtg cgatcgttgt cagtttcggc ttgatatact cgcacatatc
    14281 tatattatca tagccaataa tgctgtagtc ttcagggatg ctttttccag cttcctctaa
    14341 tccgcgatat aatccaaaag ctaattcatc atttaaagca aacacaccag ttgcagcaga
    14401 atcaagaagt tctgctgtga tctggtagcc accttgtttt gagaactgtg taggtaagag
    14461 gatcagttgg tcgtgaggga tttggtaaac atcaagagca gatttaaacc cctcaatccg
    14521 agcgtggaca ttttccggtg gattttcagg atagacaagg gcgatcgttt gatgtcctag
    14581 actaagcaag tgcatgcccg ctaggtaacc accgcgaaaa tcgtctgttc gtacggcatc
    14641 actaaatcct tcagattttt tctgatccaa aacaatgaag ggccggcctt gttttttcaa
    14701 attttcgttg attgcatctg tagagacagc agaagtcgcg ataatgaatc catccacacc
    14761 tcgacgtgtg agctcggcta gatattcgat ttctttttga tgatcagaat cagcattaca
    14821 taatatcgtg acgaaattct gcttatacaa gatatcctca attcctcgca tcagtgtact
    14881 gaagaatgga ttcgtgatat caggaacgag tacaccaatt gttttcgttt ccttggtaat
    14941 catttgtcta gcaaaatagt caggctgata gcctaattct tctttggcag ctaaaacttt
    15001 ctgaacagtt ttcggactga atttctccgt atttccattt aatatcaagg agacagttgt
    15061 gattgaaacg ccggaaagtt tagcgacttc tttgattgtg atcttttttt tcaccatgtt
    15121 catactccta tctaaatcct gtggttattt tcccatgaaa tcaaaaagaa gtaaatgaaa
    15181 atcacgtgtg ccagtactta tttgctttgt tggatagctt cgattatttg tttcgttgag
    15241 cgtactcgtg cagttttcgg aaatatcttt tttatgaaca attgatggga ttctttgtca
    15301 cgatcactgc aagcatcttc agcaagcacg atatggtacc cgtgttgaaa ggcatctaat
    15361 gcagtagcat atacaccgtt tgttgtcgct acaccgctta aaatgatagt ttcgattcca
    15421 cgccggcgaa gttgaagatc aagagaagta ccaaaaaaag cacttggatt gtattttttg
    15481 atagtcaaca tattatctgt atctttcaaa gaatccttca aaagtaaatc agtgaattct
    15541 ggaggtacag aagcagtcat tccacccata tcagtttgtt gactcaaata agggaaagtg
    15601 gagatgtcta cgttcactaa aactttcaag gcagcggtac gtttcaatgt tcttactaac
    15661 aaatcattgt ttgtcagaat agatgctgca gtgtgtggag agagtgtacc gttatttacg
    15721 atctcttttt gcatgtcgat cgcgacaaaa gcagtttttg ataaatcaaa aatcatattt
    15781 tcctttaaat cattcatgat gttccttctt tcaaataaca atgtttttta tgtaagtaaa
    15841 tcataaatcg tttatttgtc aactaaatta aaggattcct ttctttagtc tagagaaatt
    15901 cagtggtaaa ataaataagt gtgagaaaac gcattcgatg catagatata gcgagaaata
    15961 tcgtgaaaat ggaggaatca gagtgaagta tgtcgaagta aaaaatgaat acaaacgata
    16021 caaaatggga gaaacgacca ttacagcaaa tgacgatatt tctttttcaa ttgaaaaagg
    16081 cgaattagtg attatacttg gtccaagtgg agcaggaaag tcaacgattc ttaatatttt
    16141 aggtgggatg gatacacctg atgaaggtca gatcattatt gatgacacag atattgcgca
    16201 attttcagat aaacagctta ctgcttaccg acgaacagat gtagggttcg ttttccaatt
    16261 ctacaatctc gttcctaatc taacagcaaa agaaaatgtg gaattggcaa cggaagtttc
    16321 tcctgatgcg cttgatcctg tagaggtatt gaggcaagtg ggcctagctc atcgattgaa
    16381 taactttccg tcccaattat caggaggcga acagcagagg gtttcaattg ctcgtgcact
    16441 agctaaaaat cccaaacttc ttttatgtga tgaaccgaca ggcgcgctgg attttgaaac
    16501 gggcaagcaa gtattgaaat tattgcaaaa cgctagcaga caacacggca ataccgtttt
    16561 gatcattaca cataattctg ctttagcgcc catcgctgat agagtcattc acattaatga
    16621 tgctaaagta aggtctgttg aattaaatga tcatccttca tccattgatg agattgtatg
    16681 gtagggggat tttatgaaaa caagaacata tcttaaagca ggttttcgcg aaattcggca
    16741 atctaaagga cgattcattg caatcatcct tattgtcatg ctaggaacac ttttatttgt
    16801 aggtgtcaaa actgctggac ctgtcatgca aaaaacgatg gatcactatg ttggtacagc
    16861 aggactttct gatttgcaaa tcgtttctac tggtggcttg acacaaaaag atattgctca
    16921 agcagaaaag atcccagatg caaaagtaga aacaggaaaa cagatctatt attccaatac
    16981 tggcaaaaac gaagtgattc aaatattttc ttataacaaa aataacaagc agaacaagct
    17041 acaattaact gatggaaaac tcccggaaaa accaaaccag cttgtattgg atgaaaaagc
    17101 ggaagatgaa gggtataaaa tcggtgatat ttatcagatt gattcagatg aattgaaaga
    17161 aaaagagtat accatcacgg gctttgtccg ttctccattg ttcatcaaca atttggagag
    17221 aggatacgcg aacgtcggaa atggtagcgt cgattatttt gtctatttac ctgaatccgt
    17281 ttttaaaaca gatatttatt ctgtcttata tcttgatttt acaaacgtaa agaatttgaa
    17341 tacgtacagc aaagcttaca aagataaaat ggagcaaaat caagaaaagg cagaaaagta
    17401 tttacaagat cgacccgaag aacgcttaga agagctgaaa aaatcggcca acgaagaatt
    17461 agaaccggca cagcaaaaaa tagcggatgg caaagcacaa acaacgcaag caagaacacg
    17521 gttagaagat gctaaaaaac agttagcgca acagtcagca actatccagc agcttcctga
    17581 agcgcaacga acaatcgctg ttcaaacatt acctcaacaa gaagctcaac ttgctgaaca
    17641 agaaaggcaa ttagttgaga aagaacgtga actggcctct gcagaggaag aattgaatcg
    17701 aaatgcagaa aaaattgaaa atttaactaa accatcatac ctatacaatg aacgttcaga
    17761 aaatgtcggt tttcaagaat ttggtgatct gtctgatcgt atcgctgcga ttgccaatgt
    17821 ttttcctgtt ttctttttct ttattgccgc attgatcacc tttacgacaa tgacacgtat
    17881 ggtagaagaa aaccgcaggg aaatcgggac attaaaggcg ttaggttata cgaaagttga
    17941 aatcgcaaaa aaatatgcaa tctatgcaag tctagcctca gggataggta ttgttttagg
    18001 aacgatatta ggaacgaatc ttttgccaag gatcattttt gagctgtcta acgagcggta
    18061 tgatattggg agtgcgctta ttttttatga ctggccgccg attttacaag cagctgccgc
    18121 attcttcatt gccgcatttg gcgctgcaat gatcgtttta ttcaaagatt taagagaacg
    18181 gcctgctgca ttgcttcaac cgaaagcacc gaagccaggg aaacgcattt ttcttgaata
    18241 catcactccg ctgtggtcac gccttagctt taatcaaaaa atcagttacc gaaatctctt
    18301 ccgatataag tcaagaatgc ttatggcgat tatcggaatt gctggatgtg ccgggctgat
    18361 ggttgcaggc gtgggattga aagattctct aagttctgtt tcagccaaac aatttggacc
    18421 gatcattgac tatcaagcaa tcgttacatt ggattcatcc acggaagata gtcgtcaaaa
    18481 agcagcggat atgctgcaga agcaagaaaa agtaaatgag atcctagcta ttcatactca
    18541 aaccgtagaa ttgagaaaaa aaggacatgc ttcgcaaagt ctgacagtga tcgtaccaag
    18601 taaaacagct gatttggatc gttttatcca tttaactgat cttgaagaga acccacgctc
    18661 cttgcctaaa acaggaatcg ctattacgca aaaagtagca gaactatttg acttatctac
    18721 aggagatcat atttcgcttt atgatgaaga tcaaaaagaa tggaaagtcg aagtaaaaga
    18781 aatcgtgaaa aactatctag gtagctttat ttatatgtcc cctgaatatt atgcttcagt
    18841 tactgatcaa tcgatgatgg ataatgcatt tttgctgcgt acagataaga tgacaacaaa
    18901 agaagaagaa atgctttccg agaaactgct tgccacagac gctgtgacaa atacgtcttt
    18961 catgtccaaa caaatcgaaa cgcaagaaga atcaatggcg aacttagact cggttgtctt
    19021 aatctttgtc gtattatcag gactgctggc ttttatcgtc ttgtataatc taactaatat
    19081 caatatttcc gagcgtgtac gcgaattatc tacaataaaa gtccttggat tttttgataa
    19141 agaagtcacg atgtacattg ttcgagaaaa tatcatcttt actcttttag gaatcatcgg
    19201 aggatttggt gtaggatata tattgactga cttcatcttg caacaagcgt ctatggagaa
    19261 tgtcatcttt ccattagtga tcacatggtg ggcttatgtc ttatcagcag gattaactat
    19321 cgtatttacg gttattgtga tgattgttac ccatttcaaa ttaaagcata tcgatatgat
    19381 tgatgcgttg aaatcgaatg aataaaaaca gaggttgtga aatcagcgtt ttgagctaat
    19441 aacgagggtc agctgctaat tgaacaccat gaatcagagg ttgtaaaaaa gccgtcctgc
    19501 atcgagcaaa aagaatactc aaataaaatt agctcctcat gttttacatt tgagaagact
    19561 gattgccgta gataaaagct tttgaacacc atttatagaa gaaacaagaa tattttgcta
    19621 ttttaaacaa gaggatagac aaatacatgt ctgatctctt gttttttatt gggcaacaga
    19681 cctcttagaa tgtataaaga taaattttac aaacgctttc tgttcagtta taataataaa
    19741 tgataacttg tatatataag ctgtggtagg taataaatca gttgaatgga ggtaaatatc
    19801 catgacattt aaagacaaag tcatttacca aatctatccc aaatcttaca aagacagtaa
    19861 tggtgatgga attggagatt taaaaggaat caaagaaaaa ctcccgtatc tgaatgatct
    19921 aggcatcgac atgatttggc taaaccctat ttatccaagc cctcaaaaag acaacggtta
    19981 tgatatctct gattacacag ccatcgatcc tatctttggt acgatggagg aattcgaaca
    20041 gctaatagcc gaagcaaaga aataccggat agaaatcatg ctcgatatgg tgctgaatca
    20101 cgtttcgaca gaacatgaat ggttcaaaaa agcactagca ggggaccagc attatcagga
    20161 cttcttcatt ttaagagaag agccaacgga ttgggtatca aaatttggtg gaagtgcttg
    20221 gtcgccattt ggagatacgg ggaaatatta tttgcactta tatgatcgga cacaagctga
    20281 cttgaactgg cggaaccaag cagtaagaga ggaactattc aaagttgttc gtttttggat
    20341 ggaaaaaggg gtaaaaggat tccgtttcga tgttatcaat gttatcggta aagacgaaca
    20401 attgaaaaat aataaagaaa atgatggcaa acctgaatat acggacaaac caatcaccca
    20461 tacgtatcta aaagaattga accaagcaac gtttgggacg gattccacta tcatcacagt
    20521 cggtgaaatg agctctacga cgatcgaaaa ttgtatcttg tatacagaac ctagtagaga
    20581 agaattatca atggtcttta atttccatca tttgaaagtc gattatgaaa atgggaataa
    20641 atggtctact cctccatttg attttgcaga attaaagcga ctatttcata catggggaga
    20701 aaatatgagt gagggtaacg gctggaatgc tctatttttt aataatcatg atcagccacg
    20761 tgcgctcaat cgatttgtag ttatcgatca gtatcgaaaa caaggagcag agatgctagc
    20821 aaccatgatc catttgaacc gtgggacgcc ctatatttat atgggagaag aaatcggcat
    20881 gatcgaccca gactattcat cgatttccga ctatattgac gtagaaagtt tgaatgctta
    20941 taagttactc cttgaacaag gattcacaga aaaagaagcg tttcgtcgga tcaaagcaaa
    21001 atctcgcgat aactctcgaa cacccatgca atggtcagac acagaacaag caggatttac
    21061 tacagggaaa ccttggttaa aagttggagg caagctagaa gagattaatg tagaaaaaga
    21121 actatcttct gaagattcta ttttgtccta ttacaagaaa ttgattcggc tgagaaaaac
    21181 atatccaatc gttgcacaag gagattatca tgcttatgaa gcaaatcatc cggaagttta
    21241 tggctttctt cgccaatttg aaggacagca gctgcttgta ttaacaaatt tctatgataa
    21301 agatacaagg atcgccatcc cagaaggatt cacagaagcc aatattctta tcgataatta
    21361 ttccgatact gttcttcatc aaagtctgga gctaaaacca taccaggcaa ttgctttgct
    21421 gaaaaattaa aacaaaaaca caaaacgtcg ttcctacgag ctattctcgt aagatcgacg
    21481 ttttgtgttt tatttaaaat aatcttcttc agaaaatgaa gagttttcat actctccttt
    21541 atcgccataa aatttattgt agcgttgttt gaatttacgg aaaataaaag taacgagtaa
    21601 tttgactaac gtatagatag gaataccaaa aataacgccc acaattccca ttagatcacc
    21661 catgacaagt aaaacgatta agatcgtaat cggatggatc tgtaaacgat cacccattac
    21721 tcgaggaatc accaagtcac cgtggagaag ttgtacggca aaccagacaa tgacaaattt
    21781 gacagccata aaagtagagt cttggaatgc aatgatcaaa cctggaaaga aggcgataaa
    21841 cggcccaata tatggaatga tacataacaa cccagcagaa atagctaaaa cactagcaaa
    21901 ttctaatccg atcagtaaga aacaaaccca gtagactgct ccaaggataa atgaagcaat
    21961 gatttgcccc tttagaaatg aacccaattg ttgattagca atctttgtca aatttttaaa
    22021 atctgtacga aatgctggtg ggacaatcgc taaaactgac tgataaaatt tttgaggatc
    22081 tctcaaaagg aaaaaagcga caattggacc agtaaataaa gtaataaaaa tagctgtaac
    22141 agttgaaaaa atgttgctga tccgttgtgc gccactttcc caataatttc caataaatcc
    22201 agctatatca gacgtcacgt cgtcaataga agcaaaaaat tgattaaatg aatcggcaaa
    22261 aggtgttttc tccaaaaatt gtcggagact ctcttgaaaa tcttgaaaca aatctggcaa
    22321 actactgatc aatgtctttg cctgttcttc aagaaaagga ataagccata tgcttccaag
    22381 ccccaataaa aataaaataa tcacataaac gataatgact gacaagttcc tcgaaaggtg
    22441 cttgctcaaa cgtttgatga ctggttccaa aagataatac aagatcagtg caaacaataa
    22501 tgggggcaag atagtagcga taatgattcc gacaggcttg aatataaacg agatctgatg
    22561 gaaaaaataa atgatcagcg caagtaagat aactattcct aaaacatagt aagaggtttt
    22621 tcctcctaaa aagcggatca aacgattcgt tgtatctgtt tcttttttca ctctttttcc
    22681 tcctttatct ataacttcat tatagacaaa agcgggtata aaaggaaaat gatagaggtt
    22741 gattgcaaaa atatcgtcaa tcattcatat aaactttctc atcaattaaa ataagaaaac
    22801 gcttgacagt tcttttaacc atatgataaa ttaaatcctg ttgctaaaac gtaactatta
    22861 cggtttgcga aaagcgaatc ggaacattca aatgaaagaa gatgagaaga ggagtgtcat
    22921 aaatgaaacg attgataggt atattggcca tgctggtttt agcaggtatg ttaactgctt
    22981 gcggggcaag tgggaaagcc gaggacagta aagaaaaatt gagcgtgatg acgacctttt
    23041 atcccatgta tgattttaca aaagcaatcg tcggggatga aggagaagta gagttattga
    23101 ttccagcagg gactgattcc cacgattatg aaccttctgc taaagacatg gcaaagatcc
    23161 aagatacaga tatctttgtt tataatgatg aaaacatgga aacatgggtc cccgcaatcc
    23221 aaaaaacatt acaagaaggc aatgtccata cgataaaagc gacagaaggt atgcttttac
    23281 ttccgggaag tgaagaagga catgatcatg accatgaaca cggggaagaa ggccatacac
    23341 atgagctaga cccacatgta tggctagctc caagtttagc aatcaagcag gtagcaaaca
    23401 tacgtgatca gctgattgaa gcttacccag aaaaacagga agtatggaca aaaaatgcag
    23461 cagcctatac cgagaaactt caagcactcc atcagcttta tcaagaaacc tttaagcaag
    23521 caaaacaaag atcttttgtc acgcaacatg ctgcattcaa ctatctagcg ttagaatatg
    23581 gattgaatca agtatcgatt gctggtcttt cttctagtga agaaccgtct gcggcgagaa
    23641 ttgctgaact gaaacacttt gttaaagaac atggaatcaa ctatatttat tttgaagaaa
    23701 acgccaaaga tagtattgcc agaacgttag caaacgaagc aggtgtatct ttagaagtat
    23761 tgaatccttt ggaaggattg acgaatgaac aaatagaaaa tggagaaaac tatctttcta
    23821 tcatggaagc aaacctcgaa gcgttgaaaa aaacgacaga gacagaaaac ccgctagaag
    23881 atactttgac ccccaaaaaa gaaaagagcg tctacaacgg ttattttgaa gacgaggatg
    23941 tacaagatcg ctctctttca gattgggcag gaacttggca aacagtagat acgtatgtag
    24001 aagatgggac ttttgatcct gtttttgaat ataaagaaaa attgaatcag gataagacag
    24061 cacaagaata taaagaatat tatacgaagg gctatcaaac agatattgct tcaatcaaga
    24121 tcagtgagaa agagatggca tttacgttta aggatggaac aacgaaacgt tcaaattatc
    24181 gttatgtagg gaaagaaatc ttgacctacg aggcaggaaa ccgtggcgta aggtatctat
    24241 tcgaagcaga agataaggaa agtggcgctt ttagatatat tcaattcagt gatcacacga
    24301 ttgcaccggc tgactccgaa cattttcatt tatatgcagg aaatgaaagt caagaagtat
    24361 tgctgaaaga aatggagaat tggccaacat tttatccaga tacaatggat ggacgtgaaa
    24421 ttgcacagga aatgcttttg cactgatagg caaaaaa
//
LOCUS       DACDKM010000028        23866 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20834_2016
            SAMN12640370-rid9891663.denovo.028, whole genome shotgun sequence.
ACCESSION   DACDKM010000028 DACDKM010000000
VERSION     DACDKM010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640370
            Sequence Read Archive: SRR10040891
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23866)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23866)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742835.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742835.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:04:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,012
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 29 of 75
            Pseudo Genes (incomplete)         :: 33 of 75
            Pseudo Genes (internal stop)      :: 19 of 75
            Pseudo Genes (multiple problems)  :: 5 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23866
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640370-rid9891663.denovo.028"
                     /isolate="E20834_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            29..781
                     /locus_tag="IV334_001128"
     CDS             29..781
                     /locus_tag="IV334_001128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HAP8943215.1"
                     /translation="MLTEERRQKILDLLEQQKIVKSQDLVNLLNASESTIRRDLQELE
                     NSGFLERVHGGAKKEQLLNFEQNMSEKSLKNVHEKQQIAKSAAETINDEDVIYLDAGS
                     TTLEMIPFLQGKQITVVTNSVQHAANLVDLELATIILGGTIKLATNAVLGSNAIEQLK
                     NFHFNKAFMGMNGAHLEQGFTTPDPEEAAVKRLAMKNSEETFVLIDHTKFNQLSFTSV
                     APLEAATIITERCPLEFVNEFREKTTIKEAIQ"
     gene            778..1692
                     /gene="pfkB"
                     /locus_tag="IV334_001129"
     CDS             778..1692
                     /gene="pfkB"
                     /locus_tag="IV334_001129"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HAP8943216.1"
                     /translation="MIYTVTLNPSIDYIVRVDGLKLGDLNRMKEDFKLPGGKGINVSR
                     ILKRIDSQSTALGFLGGFTGEFISKWLEKEQIQSLFTTVKEDTRINIKLKSDTETEIN
                     GLGPSISDEEIDELKQALGTVGQGDIVVLSGSAPASLRKGFYQELIDIIREKGADFVI
                     DTTGDDLKNALVKKPLLIKPNNHELAELYNTTFQSVEEIIPYGKKLLAEGAQNVLISM
                     AGDGALLFTEQGTYKTNVLVRPLKNSVGAGDSMIAGFIGAYSRTKDPIEAFKWGVACG
                     SATAFSDDLAARELIDELLPEVEITKID"
     gene            1710..3641
                     /locus_tag="IV334_001130"
     CDS             1710..3641
                     /locus_tag="IV334_001130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIA"
                     /protein_id="HAP8943217.1"
                     /translation="MNIKDLMVKEAMIMDLQATDKKGAIDEMVQKMFDAGRISDIETY
                     KEGILAREAQTSTGLGDGIAMPHAKNSAVKEATVLFAKSQKGVDYEALDGQPTYLFFM
                     IAAPEGANDTHLQALAALSRLLIDADFVGKLKEANTPEEVQDLFQTAEEQKEAEEKAE
                     QETQAAEASAHPERKFVVAVTACPTGIAHTYMAEDALKKKAKEMGVEIKVETNGSEGI
                     KNRLTAEDIARAEGVIVAADKKVEMNRFDGKELINRPVSDGIRKPEELINLALSGTAP
                     VFHGDGKESSSEEGNADGTIGQRIYKDLMNGVSHMLPFVIGGGIAIALSFMIDQFIGV
                     PHDQLSNLGNYNQAASWFNQIGQAAFGFMLPVLAGFIASSIGDRPGLIVGFAAGALAN
                     TGGAGFLGALIGGFLAGYVIVFLRKLFKNLPKSLDGIKTILFYPVFGLLITGFLMLLV
                     NVPMKAINDALNGFLTSMSGSNAALLGALLAGMMAADLGGPINKAAYVFGTATLATTV
                     ATGGSVVMASVMAGGMVPPLAIFVATRLFKNKFSKTDQDAGLTNIVMGLSFVTEGSIP
                     FAAADPIRAIPSFIIGSALTGGLVGAFGIKLLAPHGGIFVVFLLSHPVMYLVFIAIGA
                     VVSGVIYGLLRKAPAVAEA"
     gene            complement(3752..5311)
                     /locus_tag="IV334_001131"
     CDS             complement(3752..5311)
                     /locus_tag="IV334_001131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClC family H(+)/Cl(-) exchange transporter"
                     /protein_id="HAP8943218.1"
                     /translation="MEKKGKIKKLDKTQIYFILKGIFVGLLTGIVVSLFRLSIEKLSD
                     LVRSIYEMSREQPIYLIGLAICCILAAFFVGYLVKNEPDIKGSGIPQVEGQLRGELSM
                     NWFSVLWKKFIGGVLSVGAGLFLGREGPSIQLGASIGQGAGQLFRSPSSEEKILISSG
                     ASSGLAAAFNAPIAGLLFVLEEIHHSFSPLVWLTSFASAITANFVSLYFFGLRPVLYL
                     GDLPSLPLKYYGSLVILGVLLGILGFIYQNVLLALPKFYNRLPLPAYFHGFVPFLLIL
                     PIGYLWPSTLGGGNQIVLAFGHASLPIIMIIGLFVLRFVFSMISYGGGLPGGIFLPIL
                     TLGALIGSFYGNIMVDFGMDPIYIKDFVVIAMAGYFTAIGKAPLTAIILVTEMVGSLN
                     HLMPLGLAALVAYIVNDLLGGNPIYESLLERLLGNTQTASLKGARTTFDFPVTAESSL
                     DGLMVRDFNWPKNMLLISIRRGNQEILTHGDTIMNVGDILVILTDEANLPRIKQEIEA
                     KSSITNLKQSF"
     gene            5466..7700
                     /gene="pcrA"
                     /locus_tag="IV334_001132"
     CDS             5466..7700
                     /gene="pcrA"
                     /locus_tag="IV334_001132"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase PcrA"
                     /protein_id="HAP8943219.1"
                     /translation="MSSKAELLNGMNPRQKEAVLHTDGPLLLMAGAGSGKTRVLTHRI
                     AYLIEEKEVNPWNILAITFTNKAAKEMKERVNAILASGGEDVWVSTFHSMCVRILRRD
                     VDFIGYNRNFTIIDSSEQLTLMKRILKELNIDPKKYDPRSILGTISQAKNSLQTPQDF
                     AKMQGSYYEEIAAKCYAAYQKELQYNQCMDFDDLIMNTIRLFEEHPDSLTYYQNKFHY
                     IHVDEYQDTNHAQYTLVNLLAGRFRNLCVVGDADQSIYGWRGADMQNILDFEKDYPDA
                     AVILLEQNYRSTKNILSAANQVIENNSNRKPKNLWTENKEGNKITYYRADNERDETRF
                     IVDRMQEEIRSNHRNYGDFAILYRTNAQSRVMEETLLKANIPYKMVGGHKFYDRKEIK
                     DILAYLNVLVNPQDSISFERIVNSPKRGIGPGSIEKLRSFASLHEWPLLEAAQNVDLA
                     NIGGKAGQQLGAFGEMIQEVTQMIPYLTVTELTKEVLDRSGYLEDLKIQNTLEAQARI
                     ENLEEFLTVTQEFDKQFEQQNEEDADAPEEKLTVFLNDLALVSDIDNLEEDASQVTLM
                     TLHAAKGLEFPVVFLIGLEEGVFPLSRALMEESELEEERRLAYVGITRAEEALYLTNA
                     FSRTLYGRTQYNRPSRFVEEIDQELLEIEGMRPTPKKTPVFAKKTAYSYKQPETAVVP
                     SKSATGGENNSWKPGDKVKHKKWGLGTVVRVSGTSKDLELDVAFPSQGVKRLLAAFAP
                     IEKA"
     gene            7717..9753
                     /gene="ligA"
                     /locus_tag="IV334_001133"
     CDS             7717..9753
                     /gene="ligA"
                     /locus_tag="IV334_001133"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HAP8943220.1"
                     /translation="MEHEMTIDEAAKRAEELRTRLNQWSREYYVEDKPTVEDYVYDKE
                     YAELVAIEEQYPDLITSDSPTQRVGGKVLEGFEKVTHDIPLYSLNDVFSKEELIAFDQ
                     RVQKAVGRVVDYCCELKIDGLSVSLRYEDGNFVRGATRGDGTVGENITENLKTVRSVP
                     IKLKEPMNIEVRGECFMPKRSFVQLNQDREAEGKDIFANPRNAAAGSLRQLDSKITAK
                     RNLDTFLYTVADFGPMQAKTQYDALEELEKIGFHTNREKRLCHSIDEVWSYIEEYHDK
                     RVDLPYEIDGIVIKVNEFSLQDQLGFTIKAPRWAAAYKFPPEEVETLIENIEWTVGRT
                     GVVTPTAIMTPVRVAGTTVSRASLHNGDYIKLKDIRLKDTVLIYKAGDIIPEVSQVVL
                     DKRPKDSEEYQLPTHCPVCGSELVHLDEEVALRCINPKCPAQMKEGLNHFVSRNAMNI
                     DGLGPRVLEQMYDKKLVADVADLYKLTEEELLTLDKIKEKSANNILTAIDNSKDNSVE
                     RLIFGLGIRHVGAKAAKILAEHFGDLETLSKSDYESIIALDTIGDIIADSVVTYFSNE
                     EVHELMNELKQAGVNFEYKGLRNAQLQEVESPFKEKTVVLTGKLTRFTREEAKETIEN
                     LGGKVTGSVSKKTDIVVAGEDAGSKLTKAQELGIEVWTEDQMADALAKSRSVEE"
     gene            9859..10164
                     /gene="gatC"
                     /locus_tag="IV334_001134"
     CDS             9859..10164
                     /gene="gatC"
                     /locus_tag="IV334_001134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HAP8943221.1"
                     /translation="MAISEEQVKHVAKLAKLAFADEELASFTDQLGKIIDMVEMLEEV
                     DTEGVPFTSNVVETVNVMRQDRAQAGWNRDELLKNVPETEDGFIKVPAIIDNGEAGA"
     gene            10164..11636
                     /gene="gatA"
                     /locus_tag="IV334_001135"
     CDS             10164..11636
                     /gene="gatA"
                     /locus_tag="IV334_001135"
                     /EC_number="6.3.5.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HAP8943222.1"
                     /translation="MTELHNRSLEELHHMLVSKEITVQDLTKTTFERIKETEPEVEAF
                     ITLNEEKALEQAKALDEKGIDESNVLAGIPIGIKDNIVTKDILTTAASKILYNFEPIY
                     DATVMDKIYQADMIPVGKLNMDEFAMGGSTETSYFKKTKNAWDPTKVPGGSSGGSAAA
                     VAAGQIPVSLGSDTGGSIRQPAAFNGIVGMKPTYGRVSRFGLIAFASSLDQIGPMTRT
                     VKDNALALTAISGYDEKDGTSSGVSVPDFAEGLTGDIKGMKIALPKEYLGEGVAPGVK
                     EAVLKAAETFRSLGAVVEEVSLPHSKYGVAVYYIIASSEASSNLQRFDGIRYGYRSEN
                     VKSLDDVYVNSRSEGFGEEVKRRIMLGTFSLSAGYYDAYFKKAGQVRTLIKRDFEKVF
                     ADYDLIIGPSSPTVAFGIGENINDPITMYMSDILTIPVNLAGLPGMSVPAGLSEGLPV
                     GLQIIGNYFDEKTMYQAAYAFEQATEFHKQQPAILGGKDE"
     gene            11633..13063
                     /gene="gatB"
                     /locus_tag="IV334_001136"
     CDS             11633..13063
                     /gene="gatB"
                     /locus_tag="IV334_001136"
                     /EC_number="6.3.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HAP8943223.1"
                     /translation="MNFETVIGLEVHVELKTNSKIFSPAPAHFGAEPNSNTNVIDWGY
                     PGVLPVMNKAALEFGMKAALALNCEISKDTHFDRKNYFYPDNPKAYQISQFDQPIGHD
                     GWIEIEVEGKKKKIRIERVHLEEDAGKNMHGIGGYSYVDLNRQGTPLIEIVSEADMRS
                     PEEAYAYLEALRSIIQFTEVSDVKMEEGSMRCDANISLRPYGQEEFGTKAELKNLNSL
                     SFVKKGLAFEEKRQAKVLLSGGEIQQETRRFDETTNKTVLMRVKEGSSDYRYFPEPDI
                     PKFVIDDEWIQKVKASLPEMPASRRERYIREFGLPEYDAMVLTLTKEMSDFFEAVLEN
                     GADAKQASNWLMGEVSAYLNSEKLELAETKLTPENLAGMIRLIADGTISSKIAKKVFR
                     ELITNGGEAQEVVEKNGWVQLSDPNKLLPIINEILDNNQQSVDDFKNGKDRAVGFLVG
                     QIMKATKGQANPGVVNKLLKDELAKR"
     gene            13073..14128
                     /locus_tag="IV334_001137"
     CDS             13073..14128
                     /locus_tag="IV334_001137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HAP8943224.1"
                     /translation="MKKARVIYNPTSGKELLKKNLADILQALEEAGFEASAYATTPEP
                     DSAKNEACRVAELGFDLVVAAGGDGTINEVVNGIAPLEHRPKMAIIPAGTTNDYARAL
                     KIPRDNIKAAAEVIKKHQTVKMDIGLSDNSYFINIAAGGYLTELTYEVPSELKSIFGY
                     LAYLAKGAEMLPRVKPIKMRLKYDEGEYIGNASMFFLGLTNSVGGFEQIAPDAKLDDG
                     KFSLIIVKTANIFEILHIAALMLNGGRHVDDPRIIYTKTSSLFVETEDDINRPVMINL
                     DGEYGGEAPMHFQNLHQHIEFFANTDQIPDEAITGTDEEELEVVSKEFVKEVERLTDE
                     DIDGDGKIADETPNQKN"
     gene            14239..15612
                     /gene="rlmD"
                     /locus_tag="IV334_001138"
     CDS             14239..15612
                     /gene="rlmD"
                     /locus_tag="IV334_001138"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HAP8943225.1"
                     /translation="MTEQQIVKKNERYTVEIVDLSYEGMGVAKIDGYPLFIENALPGE
                     EVEILVVKAGNKFGYGKVETFITQSPDRQPVENNALLRIGIAPLAHLKYEQQLVFKQK
                     QVEHVMAKIAKMPEVPVLPTVGMAEPFGYRNKAQIPVRRIDGQLATGFYKKNSHDLVP
                     IEDFYIQDPVIDQAIKVVRDILQRFQVRGYNEEKNEGQLRHIIIRRGHYSHEMMVVLV
                     TRKEKFFKSKEIAEAIHEELPEVVSVIQNINEEKTNVILGDKEKVLYGRDYIEDQLLG
                     KTYRISAKSFYQVNTQQAEYLYQTAIEFAELDKKDIVIDAYSGIGTIGLSLADRVDKV
                     YGMEVIPEAIEDAQFNALNNKIENVHYEVGKAEKVMKKWQEKGIKPSVIVVDPPRKGL
                     DHHFIESAIEMSPERIVYISCNPATFARDAKLFAEAGYAVKKVQPVDLFPQTHHIELV
                     ASFVKGE"
     gene            15615..15743
                     /locus_tag="IV334_001139"
     CDS             15615..15743
                     /locus_tag="IV334_001139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HAP8943226.1"
                     /translation="MDQKEKMKELIKKKQGGGRSKQMETPKNDRKNMRKGPKIFNK"
     gene            16183..16371
                     /locus_tag="IV334_001140"
     CDS             16183..16371
                     /locus_tag="IV334_001140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HAP8943227.1"
                     /translation="MEEHQQVIDRIYEATRYLPLDRLCLSPQCGFASTEEENVLFEEQ
                     WKKFLLSKALQKKFGENH"
     gene            16449..17462
                     /locus_tag="IV334_001141"
     CDS             16449..17462
                     /locus_tag="IV334_001141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAP8943228.1"
                     /translation="MKKKILKFIGWFFGIILGIAFAIFLAFQLSPRPGAFLINHMFSN
                     TVQITDKKTFDQAEKNVAKKTDLTYESKYKENTYDIYYPKNSKGPVPVLFWVHGGGFV
                     GGDKSGVKEFATKLVSDANIAVVAMNYELAPDSEYPNQVLQVNELIKDLLAEEKDDKL
                     LDMEQLFFGGDSAGSQIALQYAAIQTNDAYAKQMKIKQLLPKDSLKGTISYCGPVDLK
                     LTAQQHSDDRFMKFFVKTVAWSLIGTKDWKTSEQLKEASVADHVSKEFPPTYITDGNA
                     YSFQEQGQALENRLTELKVPVQSLFYTDTKKEITHEYQFDYTLKESQNCYQQTLDFVN
                     KYK"
     gene            complement(17529..18563)
                     /locus_tag="IV334_001142"
     CDS             complement(17529..18563)
                     /locus_tag="IV334_001142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAP8943229.1"
                     /translation="MVGIRDVARKANVSPATVSRVLNNDQTIHVAQTTRQRIVEAAAA
                     LDYDISTRKYIKKRMPSIGVISTIGKASEEQDIYYKHLRVGMEEEARRLHLGMNRIYN
                     LSDNPKQWEDLDQLGAVIVVGTVTKESITALLKQNMQIVVVDNPDIQQEVDMVYADLE
                     RMTKTVLELFLENGHKRIAYIGGYKVDMDEFGGKQISINEKRMRAYQHFMIDHHLEKY
                     MSYHLGGWTEKQGEVLATNLLENESPLPTAILVGSDPMAIGVYRALKQKNIQIGEEIV
                     VASFDNIETIANLSPGLTSVQINAKSIGQAAVRMALERIDGIRDEPIILTYPARLIVR
                     ESFIPRTKRY"
     gene            18678..20903
                     /locus_tag="IV334_001143"
     CDS             18678..20903
                     /locus_tag="IV334_001143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HAP8943230.1"
                     /translation="MDITHRKTDKNFILFDEKSRVFHLRNTFLSYLIKIEESNVLAHI
                     YFGKPVKQYKDNKNYPRRDRGFSGNVPLNPDRSLSKDTLPQEYSSHGSMDFRTPASII
                     QRKNGSDLLDLRYDSHYITDGKPEIEGLPQTYVMDKSEAQTLVISLKDRETAIYFDLF
                     YTIFTDRAVITRSVKIRNETGETIKLEKAASFQLDFAHTRRFDEVIALPGAHVNERQI
                     SRQSVLSGTKVFESRRGTSSHHMNNFIALVHHHTTENTGEAIGLQFVYSGNHSFELEK
                     DQINQLRVVGGINSHRFSWELNAGQSFQTPEMILSYSSQGLNKMSQIHHELLRERIAR
                     GRHQFAERPILVNNWEATYFDFNSEKIKAIIDEAKELGIEMFVLDDGWFGKRDADNSS
                     LGDWFEYEGKLTNGLREIADYAHSKGLKFGLWFEPEMISVDSELYRTHPDFLMQEPGR
                     MPSASRSQHVLDFTRLDVRQTIEKQMRKILDTIPLDYIKWDMNRSLSDVYSITLDPQR
                     QGEVAHRYMLGLYELLEHLTTDYPEILWEGCSGGGGRFDAGFIYYMPQSWTSDNTDAV
                     ERMKIQYGTSLAYPISSITAHVSAVPNHQTDRSTSLKTRGETAMSAVFGYELDLTKLS
                     PEEKKQVKDQIISYQTIRPVIQYGHYYRLASPFEENIAAWMFVSPKQDEAIVFLGRIL
                     ASAQPAFHEVYLMGLDDEALYQEQTSKRIFSGAELMTVGLYFPDFQGDFQTELLHFKK
                     L"
     gene            20921..22804
                     /locus_tag="IV334_001144"
     CDS             20921..22804
                     /locus_tag="IV334_001144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2"
                     /protein_id="HAP8943231.1"
                     /translation="MKANIKWLDDPQVFRVNQSNAHSDHVCYATREEVETTSSLKQSL
                     NGSWRFCYSPNPSVRPKTFYKKDFDACSFDEITVPSHIELANYDKIHYINTMYPWEGH
                     VFRRPPFTTSPEKEAEGIFSAAKDNPVGSYLTTFRLEEGLKGKQISIHFAGVEQAMFI
                     WLNGHFIGYAEDSFTPSEFDLTPYLEEENILAVEVYKRSTAAFLEDQDFFRFFGIFRD
                     VFLVARGQNHLEDLWIKPRLDDKCQNGKLEVDLSFEQVAKNSQLNVAILDPDGKEIYQ
                     QQYPLKEQMTIRIDGFENVALWGHFQPNLYKLELETIDEEKRVTEYIDYSFGFRKIEI
                     KDKIIYFNGKRMIINGVNRHEWHPKKGRAITIEDMEKDLAIIKNAKINGVRTSHYPNQ
                     ITWYYLCDQAGIHVMAETNLESHGSWQKMGAFEPSYNVPGAVPQWQAAVLDRAKTNFE
                     TLKNHPSILFWSLGNESYAEENIRQMNDYFKKVDPDRLVHYEGNFPNPAFEDSISDVK
                     SCMYAPPEEIIDYLENKAEKPFILCEYMHDMGNSLGGMDSYMTLLDRYEMYQGGYIWD
                     FKDQAIQVTDPITGREVMRYGGDFDDKPSDYEFSGNGIVFADGQEKPAMQEVRYYYEK
                     YSK"
     gene            22785..23759
                     /locus_tag="IV334_001145"
     CDS             22785..23759
                     /locus_tag="IV334_001145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase small subunit"
                     /protein_id="HAP8943232.1"
                     /translation="MKNTVNKSHMDTEKVAIVFGDCTLGVKSGNTHYIFSYTRGGLES
                     LNKNGKEWLYRETLPVFWRATTDNDRGSGFGFKSGMWLGAGLYPKVKEIEVLVDNKKI
                     DLPIAPVNNQYSSNEYAETVEIIFTLEINTVPKTQVTISYQIAGEGKMLVNMHYYGQK
                     GLPELPALGLRFIMPTKAKFFEYEGLSGETYPDRMAGGVEGIYKVAGLPVSPYLVPQE
                     CGMHMETKWVELTRNQTLDNTDKDQEDFSLRFEQQDTPFAFSALPFKAEELEQATHIE
                     ELPPERRTVLTIFAKVRGVGGINSWGADVESPYRISSEEDHEIGFIIQ"
BASE COUNT         7975 a         4368 c         5180 g         6343 t
ORIGIN      
        1 tattatttgt attgaggtga tcgtgaaaat gcttacagag gaacgcagac aaaaaatcct
       61 agacttgctt gaacagcaga aaatcgtgaa atcacaagat cttgtaaacc ttttgaatgc
      121 ttctgaatca acgataagac gtgatttaca agagctggag aatagtgggt ttctagaaag
      181 agttcatggc ggagctaaga aagagcaatt attgaatttt gagcagaata tgagtgaaaa
      241 atcactcaaa aacgttcacg aaaaacagca aatcgccaaa tcggcagctg aaaccattaa
      301 tgacgaagat gtcatttatt tagacgctgg ctctactacg ttagaaatga ttccttttct
      361 gcaaggaaaa cagatcacag tagtgacaaa ttccgttcaa catgcggcaa atttagttga
      421 tttggagctt gctaccatta ttttaggtgg aacgataaag ctggcaacta atgctgtttt
      481 aggatctaat gcgatagaac aactaaaaaa ttttcatttt aataaagcat ttatgggaat
      541 gaacggtgcc catcttgaac aaggattcac gactccggat ccagaagagg cagctgttaa
      601 acgtttagcc atgaaaaatt ccgaagagac atttgtattg attgatcata ccaaattcaa
      661 ccagttgtcc ttcacctctg ttgcaccttt ggaagcagcc acgatcataa ccgagcgttg
      721 tccattagaa ttcgttaatg aattccgtga aaagacaaca attaaggagg caatacaatg
      781 atttacacag taacacttaa tccatcaatt gattatatcg tacgcgtaga tgggttaaaa
      841 ctaggcgatt tgaaccgaat gaaggaagac ttcaagttac ctggaggaaa agggatcaat
      901 gtttcccgta ttttgaaacg gatcgattct caatctacag cactagggtt tttaggaggg
      961 ttcactggcg aatttatttc gaagtggctt gaaaaagaac aaatccagtc tttgttcaca
     1021 accgtaaaag aagatacaag aatcaatatc aaactaaaat cagacactga aactgagatc
     1081 aatggcttag gcccatctat ttcagatgaa gaaatcgatg agttgaagca agcattagga
     1141 acagtaggac aaggagatat cgtcgtattg tcaggaagcg cgccagctag cctgcgaaaa
     1201 ggattttatc aagaactgat tgatatcatt cgagaaaaag gtgcagattt cgtgattgat
     1261 acaacaggtg atgacctaaa aaatgcactt gttaagaaac cgttgttgat caaaccaaac
     1321 aatcacgaat tagctgagct ttacaacaca accttccaat ctgtagaaga aatcattccg
     1381 tatgggaaaa aattactagc tgaaggggca cagaatgtgc tgatctcaat ggctggagac
     1441 ggtgctttgc tcttcacgga acaaggaact tataaaacga atgtattagt tcgcccattg
     1501 aaaaattcag taggtgcagg ggattcaatg attgctggct ttattggggc ttacagccgt
     1561 acgaaagatc caatcgaagc ctttaaatgg ggagttgctt gcggaagcgc aacagctttt
     1621 tctgatgact tggcagctcg cgaattgatt gatgagttgc tgccagaagt tgaaatcaca
     1681 aaaattgatt agtttcaagg aggataatca tgaatatcaa ggatttaatg gtcaaagaag
     1741 cgatgatcat ggatttgcaa gcgactgata aaaaaggcgc aatcgatgaa atggtccaaa
     1801 aaatgtttga tgccggccgg atttccgata tcgaaacata taaagaagga attctagccc
     1861 gcgaagcaca aacttctaca ggtcttggtg atggaatcgc catgcctcac gcgaaaaaca
     1921 gtgcagttaa agaagcaaca gttttattcg caaaaagcca aaaaggagta gattatgaag
     1981 ccttagacgg acagccaact tatctgtttt tcatgatcgc agctccagaa ggcgcaaacg
     2041 acacacattt gcaagcatta gcagcactat ctcgattgct gattgatgca gatttcgtag
     2101 gaaaattaaa agaagcaaat acaccagaag aagtccaaga cttgttccaa acagctgaag
     2161 agcaaaaaga agcagaagaa aaagcagaac aagaaacaca agctgcagaa gcttctgctc
     2221 atccagaaag aaaattcgtt gttgccgtta cagcttgtcc aacagggatc gctcacactt
     2281 acatggcaga agatgctttg aagaaaaaag cgaaagaaat gggtgtggag atcaaagtcg
     2341 aaacgaacgg ttcagaagga atcaaaaacc gattgactgc tgaagacatc gcccgtgcag
     2401 aaggtgtaat cgttgcagca gacaaaaaag tagagatgaa tcgctttgac ggaaaagaac
     2461 tgatcaatcg tcctgtcagt gatggtatcc gtaaaccaga agagttgatc aatctagccc
     2521 ttagtggtac tgcaccagta ttccatggtg atggaaaaga aagcagttca gaagaaggaa
     2581 atgcggatgg tactatcgga caaagaattt acaaagattt gatgaacggc gtatcacaca
     2641 tgttaccttt cgttattggt ggaggaatcg ctattgcgct ttctttcatg atcgaccaat
     2701 ttattggcgt gccacatgat caattatcaa atctagggaa ttacaatcaa gctgctagct
     2761 ggttcaacca aatcggacaa gctgcatttg gtttcatgct gccggtatta gcaggattta
     2821 ttgcttcaag tatcggtgac cgccctggtt tgatcgttgg atttgctgct ggtgcattag
     2881 ctaatacagg cggtgcagga ttcttaggtg cactgattgg tggattctta gctggttatg
     2941 tcatcgtatt tttacgtaaa ttattcaaga acttaccaaa atcattggat ggaattaaaa
     3001 cgatcctctt ttatccagtg tttggcttac tgattactgg tttcttgatg ttgctggtca
     3061 atgtccctat gaaagcaatc aatgatgcat tgaacggttt cttgacaagt atgagtggtt
     3121 cgaatgctgc tttacttggt gcattattag ctggaatgat ggcggcagat ttaggcggtc
     3181 caatcaacaa agccgcttat gtgtttggta cagcaacttt agctacgact gttgcaactg
     3241 gcggaagtgt cgtgatggct tctgttatgg ctggaggaat ggtacctcca ttggcaattt
     3301 tcgttgctac acgtttgttt aagaataaat tcagcaaaac agatcaagat gctggtttga
     3361 cgaacatcgt gatgggacta tcgtttgtca cggaaggatc gattccattt gcagcagctg
     3421 acccgattcg tgccattcca agttttatta ttggttctgc tttaactggc ggattagtcg
     3481 gagctttcgg aatcaaatta ctggctcctc acggcgggat tttcgttgta ttcttactga
     3541 gtcacccggt catgtatttg gtgtttattg ctatcggagc agtcgtatct ggtgtgattt
     3601 atggtttatt gagaaaagct cctgcagtag cagaagcata gaaataaaaa agaagaggct
     3661 gggttttccc agcctcttct ttttttatag aatcatatag aatcaagtag tgtagatgaa
     3721 tagttgttgt aaaatagttt ttccatgttc gttaaaatga ttgcttcaaa tttgtgatag
     3781 aagatttagc ctcgatttcc tgcttgattc ttggcaagtt tgcttcatca gtcaagataa
     3841 ccaaaatatc tcctacattc atgatcgtat caccgtgagt caagatttcc tgatttcctc
     3901 tgcgtataga gattagcagc atatttttag gccaattgaa gtctcgtacc attaatccgt
     3961 ctaaagaact ctctgccgtt actggaaaat caaaagttgt ccttgcacct tttagtgagg
     4021 cggtctgagt atttccaagt agacgttcca ataatgattc gtaaattgga ttaccgccga
     4081 gaaggtcatt gacgatataa gcaacaagag cagccaatcc taaaggcatc agatgattaa
     4141 gagagccaac catttctgtc actaaaataa ttgctgtcag cggtgctttt ccaatcgctg
     4201 tgaaataacc agccatggca ataacgacaa aatccttgat gtagattgga tccatcccga
     4261 aatcaaccat aatgtttcca taaaagctac cgattaaagc ccccaatgtc aatatcggaa
     4321 ggaaaatccc ccctggaaga cccccaccgt aagaaatcat ggaaaaaacg aatcgaagaa
     4381 caaacaaacc gataatcata atgattggca aggaagcatg tccaaaagct aaaacgattt
     4441 gattgcctcc gcctagagta ctaggccata aataaccaat tggcaaaatc agcaagaaag
     4501 gaacgaaccc gtgaaaataa gctggtaaag gcaatcggtt gtaaaatttt ggcaaagcca
     4561 gcaagacatt ctgataaata aaacctaaga tgcctagaag tactcctaag atcactaagg
     4621 aaccataata tttcaacggc aaagaaggaa ggtcacctag atataaaaca ggtctcaagc
     4681 cgaaaaagta aagagagacg aagttagcag tgattgctga agcaaatgat gtcagccaaa
     4741 caagtggaga aaaggagtga tggatttctt ctaaaacaaa gagtaatcct gcgataggag
     4801 cattgaatgc tgctgcaagt ccgctacttg caccgctaga aattaaaatc ttttcttctg
     4861 aagatggact ccgaaacaat tgtccagctc cttgtccaat ggatgcgcca agctgtatag
     4921 aaggtccttc tctacctaga aaaaggcccg caccaactga aagtacaccg ccaatgaact
     4981 ttttccataa aacagagaac cagttcattg ataattcccc gcgaagctgt ccctctactt
     5041 gtggaatccc gcttcccttg atatccggtt catttttgac cagataaccc acgaaaaatg
     5101 ctgcaaggat gcagcaaata gctaggccaa ttaagtaaat cggctgttcc cgactcattt
     5161 catagatact tcgaactaaa tcgcttagct tttcgataga taatcgaaac aggctaacga
     5221 cgattcctgt cagcagtcca acaaatattc cttttagaat aaaatatatc tgagtcttat
     5281 ctaatttctt gatttttcct tttttttcca ttcaaacgcc tcgctttctt ttaatttagc
     5341 gcgaaatcaa cgaattgaca aggaaaaatc cataaaaaga aacaaatgat tttgaactag
     5401 ctagtttaat gaaagccaag aaaatgctat aattaacaag attgataaaa gaaaggatgt
     5461 tgttaatgtc atccaaagct gaattattaa acgggatgaa cccccgtcag aaagaagctg
     5521 ttcttcacac tgacggacca ttactgctaa tggcaggtgc aggaagtgga aagacgagag
     5581 tgctgacaca ccgcattgcc tatttgattg aggaaaaaga agtcaatcct tggaacatcc
     5641 tagcgattac ttttaccaat aaggctgcaa aagaaatgaa agaacgtgtc aatgcgattc
     5701 tcgcatctgg aggcgaagat gtctgggtat cgacttttca ctcgatgtgt gtgcgtattt
     5761 taagaagaga tgttgatttt atcgggtata accggaactt tacaatcatc gattcttctg
     5821 aacagctgac cttgatgaaa cggatactaa aagaactgaa cattgatccg aaaaaatatg
     5881 atcctcgaag tatattagga acaatctctc aagcaaaaaa cagcctgcag acccctcagg
     5941 attttgcaaa aatgcaaggc agttattacg aagagattgc ggcaaaatgt tatgctgctt
     6001 accaaaaaga gcttcaatat aatcaatgta tggactttga cgacctgatc atgaatacga
     6061 ttcgattatt tgaagaacat cctgattcac tcacgtatta tcaaaacaaa ttccattata
     6121 ttcatgtaga tgagtaccaa gatacaaatc acgcacagta tacgctagtc aatttattgg
     6181 caggtagatt taggaacctg tgcgtcgtgg gagatgcaga tcaaagtatt tacggctggc
     6241 gcggtgcaga tatgcagaac atcttggatt ttgaaaaaga ttatccagat gctgcagtaa
     6301 ttttgctaga acaaaactat cgctccacga aaaacatcct tagcgcagca aaccaagtca
     6361 ttgaaaacaa tagcaatcga aaaccaaaaa acttgtggac agaaaacaaa gaaggaaaca
     6421 agatcactta ctaccgtgca gataatgaac gagacgaaac ccgttttatc gtagaccgga
     6481 tgcaagaaga gattcgaagc aatcatcgga attacggcga ctttgccatc ttgtaccgga
     6541 cgaacgcaca atctcgtgta atggaagaaa ctctgttgaa agcaaatatt ccttataaaa
     6601 tggttggagg gcacaagttc tacgatcgta aagaaatcaa agatatttta gcgtatttga
     6661 atgtgctggt aaatcctcaa gattcaatca gttttgagcg tatcgttaat tcgcccaaac
     6721 gaggcatcgg accaggatct atcgaaaaac tacgcagttt tgcttctctt catgaatggc
     6781 cactgcttga agcagcgcaa aatgtggatc tggcaaatat cggtggaaaa gcaggacagc
     6841 aattaggtgc atttggagag atgatccaag aagtcactca gatgatccca tacttgacag
     6901 tgacagaatt gacgaaagag gttttggatc gtagcggata tctagaagac ttaaaaatcc
     6961 aaaatacatt agaagcacaa gcacgcatcg aaaacttgga ggaattttta acggtcacac
     7021 aagaattcga caaacaattc gaacagcaaa atgaagaaga cgctgatgca ccagaagaaa
     7081 aactaacggt cttcttaaat gatctggcac ttgtttccga cattgacaat ttagaagaag
     7141 atgcttccca agtgacattg atgactttgc atgcagccaa aggattagaa tttccagttg
     7201 tattcctgat tggtctagaa gagggggttt ttcctttatc tcgagcattg atggaagaaa
     7261 gcgaactgga ggaagaacgc cgtctcgcat atgtggggat cacgcgagca gaagaagcgt
     7321 tgtatttaac aaatgctttc tccagaacat tatatggacg gacgcaatat aaccgacctt
     7381 ctcgttttgt agaagaaatc gatcaggagt tactggaaat cgaaggcatg cgtcctacgc
     7441 ctaaaaaaac gcctgtattc gctaaaaaaa cagcatatag ctataaacag ccagagacag
     7501 cggttgttcc ctctaaaagt gctactggag gggaaaataa cagctggaaa cctggagata
     7561 aagtcaaaca caaaaaatgg ggtctaggaa cagtcgtccg agtaagcggg acgtcgaagg
     7621 atctagaact agatgtggct ttcccaagtc aaggagtaaa acgtcttttg gctgcctttg
     7681 cgccaattga aaaagcttag tagtggaggg taaatgatgg aacatgaaat gacgattgac
     7741 gaagctgcta aacgagcaga agaacttcgt acaagactga accaatggtc tcgcgaatac
     7801 tacgtagaag ataaaccaac agtggaagac tatgtttatg ataaagaata cgcagaatta
     7861 gtagcaatcg aggaacaata tcctgatttg atcacgtctg attctcccac tcaacgagta
     7921 ggcggaaaag tattagaagg ttttgaaaaa gtcactcatg atattccgct ttacagtttg
     7981 aacgacgttt tcagcaaaga agagctgata gcttttgatc aacgtgtaca aaaagcagtg
     8041 ggacgtgtag ttgactattg ctgtgaacta aaaatagatg gactttccgt ttctttgcgt
     8101 tatgaagatg gaaatttcgt gagaggtgcg actcgcgggg atggaactgt aggagaaaac
     8161 atcactgaaa acctaaaaac tgtccggtcg gtcccaatca aattaaaaga accaatgaac
     8221 atcgaagtaa gaggcgaatg tttcatgcca aaacggtcat ttgtccaatt gaaccaagac
     8281 agagaagcag agggaaaaga tatctttgcg aatcctcgga acgccgcagc tggttctttg
     8341 cgtcagttag attctaaaat cacagcgaaa agaaatctgg acactttttt atacacagta
     8401 gctgatttcg gtcctatgca agcaaaaacc cagtatgatg cgctagaaga gctagaaaaa
     8461 attggattcc acacgaatcg ggaaaaacgt ctatgtcatt cgattgatga agtgtggtca
     8521 tacattgaag aatatcatga caaacgtgtg gatttgccct atgaaatcga tggaatcgtc
     8581 atcaaagtga atgaattctc gctacaagat caacttggtt ttactatcaa agcaccgaga
     8641 tgggcagcag cttacaagtt tccgccagaa gaagttgaaa cgctaatcga aaacatcgaa
     8701 tggacagtcg gtcgaacagg ggtagtaacg ccaacagcta tcatgacacc agtccgagta
     8761 gcaggaacga cagtaagccg tgccagcctg cataacggtg attatattaa gttgaaggat
     8821 atccgcttga aagatacagt tctgatttat aaagcgggag acatcatccc agaagtttcg
     8881 caagtcgtgt tagataaacg acctaaagac agcgaagaat accaattgcc tacacattgt
     8941 ccagtttgtg gaagtgaact tgtccatttg gatgaagaag tagccttacg gtgcatcaat
     9001 cctaaatgcc ctgctcaaat gaaagaaggt ttgaaccact ttgtttcaag aaatgcgatg
     9061 aatatcgatg ggttggggcc gcgcgtacta gaacagatgt atgataaaaa actagtagca
     9121 gatgttgctg atctttacaa attgacagaa gaagagttat tgacgttgga caaaataaaa
     9181 gaaaaatcag caaataatat tttgacagca atcgacaaca gcaaagataa ttcggtcgag
     9241 cggttgattt tcgggttagg aatccgacat gtcggagcga aagcagcaaa aattttagca
     9301 gaacatttcg gtgatctaga aacattgagt aaaagtgact atgagtcgat tattgcgctt
     9361 gatacgatcg gtgatatcat tgccgacagt gtagtcactt atttcagtaa cgaagaagtc
     9421 catgaattga tgaatgaatt aaaacaagca ggcgtcaatt tcgaatacaa agggcttcga
     9481 aatgcccaat tgcaagaagt cgagtctcct tttaaagaaa aaacagtagt ccttactggt
     9541 aaactaacac gatttactcg tgaagaagca aaagagacga tcgaaaatct tggcgggaaa
     9601 gtgactggta gcgtatcgaa aaaaacggat atcgtggtag caggagaaga tgctggcagc
     9661 aagctgacca aagcacaaga attaggaatc gaagtttgga cagaagatca aatggcagat
     9721 gcattggcta aaagtcgctc agtagaagaa tgaaaaatga gttttttcag acaaatttca
     9781 ataaacaatt tcagacacgg cactcttgtg ttaaaataga gagcaaagtt tttaactcgt
     9841 ttagaaagaa ggggtagtat ggcgattagt gaagaacaag tgaaacacgt tgccaaatta
     9901 gcaaaacttg cgttcgcaga tgaagaactt gcaagtttta ccgaccaatt aggtaaaatc
     9961 atcgatatgg ttgaaatgct ggaagaagta gatacggaag gtgttccatt tacgtcgaat
    10021 gtggttgaaa cagtaaacgt gatgcgacaa gatcgggcgc aagctggatg gaaccgcgat
    10081 gaattattga aaaatgtgcc ggaaacagaa gacggcttta tcaaagtacc cgcaatcatc
    10141 gataatgggg aggctggcgc ataatgacag aattacacaa tcgttctcta gaagaactgc
    10201 atcatatgct tgtatcgaaa gaaatcactg tacaagattt gacaaaaaca acatttgaac
    10261 gaatcaaaga aacagagcct gaggtcgagg cattcattac attgaacgaa gaaaaagcat
    10321 tggaacaggc aaaagcttta gatgaaaaag ggattgatga aagcaacgtc cttgctggta
    10381 ttccaattgg tatcaaggat aatatcgtaa caaaagatat cttgacaacc gctgcatcta
    10441 agatccttta caattttgaa ccaatttatg atgcaacggt catggataaa atctatcaag
    10501 cagatatgat ccctgttggt aagctaaaca tggatgaatt tgctatgggt ggaagtacag
    10561 aaacttctta tttcaagaaa acaaaaaatg cttgggaccc aacaaaagta cctggtggct
    10621 cttctggtgg ttctgcagca gcagttgcag ctggacaaat tccagtttct ttaggtagtg
    10681 atactggggg aagtatccgc caaccagcag cttttaacgg catcgtaggg atgaaaccta
    10741 cttatggccg tgtctcacgt tttggtttga ttgcctttgc ttcatctctt gaccaaatcg
    10801 gaccaatgac ccgtacggtg aaagataatg cattagcatt gacagctatc agcggttatg
    10861 acgaaaaaga cggtacttct tcaggcgttt cagttcctga ttttgcagag ggactgacag
    10921 gcgacatcaa aggaatgaag attgctttac ctaaagaata cttaggcgaa ggtgtggccc
    10981 ctggcgtaaa agaagctgta ttgaaagcag cagaaacatt ccgttcatta ggtgctgttg
    11041 tggaagaagt cagcttgcct cattcaaaat acggtgtggc tgtttattat atcattgcct
    11101 catcagaagc aagttccaat ttgcaacgat tcgatggtat ccgctacggt tatcgctctg
    11161 aaaatgtgaa atcattagat gatgtatatg tcaactcacg ttcagaagga ttcggtgaag
    11221 aagtaaaacg ccgtatcatg ttaggtacat tctccttaag cgcgggttat tatgacgctt
    11281 acttcaaaaa agcaggacaa gtccgtacat tgatcaaacg agattttgaa aaagtctttg
    11341 cagattatga cttgatcatc gggccatctt caccaacagt tgcattcgga atcggtgaga
    11401 acattaacga tccaatcaca atgtacatga gtgacatctt aacgatccct gtcaacttgg
    11461 ctggacttcc aggtatgtct gtaccggcag gtttatcaga aggattacca gttggattgc
    11521 agatcatcgg taattatttt gatgaaaaaa cgatgtatca agcggcatat gcgtttgaac
    11581 aagcaacaga attccataaa caacaaccag ctattttagg agggaaagac gaatgaattt
    11641 tgaaacagtc attggactag aagtccacgt cgagttaaaa accaactcaa aaattttctc
    11701 tccagcgcca gctcattttg gtgcagaacc aaacagcaat acaaacgtga tcgactgggg
    11761 ttatccaggc gtgctgccag taatgaataa agctgcgctg gaattcggga tgaaagcagc
    11821 tttggcatta aactgtgaaa tctctaaaga tactcatttt gaccggaaaa attactttta
    11881 cccagataat ccaaaagcat atcaaatttc ccaatttgat caaccaatcg gtcatgacgg
    11941 atggatcgaa atcgaagtag aaggtaaaaa gaaaaaaatc cggatcgaac gtgtccattt
    12001 agaagaagat gctggaaaaa acatgcatgg tattggcggt tattcttacg ttgatttgaa
    12061 ccgacaaggc acgcctttga tcgaaatcgt ttctgaagct gatatgcgtt caccagaaga
    12121 agcatacgct tatctagaag cattgcgatc aattatccaa tttacagaag tcagcgatgt
    12181 gaaaatggaa gaaggctcta tgcgctgtga tgccaatatt tctcttcgcc cttatggaca
    12241 agaagagttc ggtacgaaag cagaattgaa aaacttgaac tctttatctt tcgtgaaaaa
    12301 aggtttagct tttgaagaaa aacgtcaagc aaaagttctt ctttcaggag gagaaatcca
    12361 acaagaaaca cgccgttttg acgaaacaac gaataaaaca gttttgatgc gtgtcaaaga
    12421 agggtctagt gattatcgtt acttcccaga gcctgatatc ccgaaattcg tgatcgatga
    12481 cgaatggatt caaaaagtaa aagctagttt gcctgagatg ccagcttctc gtcgcgaacg
    12541 ctatatccgt gagtttggac ttccagagta tgacgcaatg gtattgacac ttacaaaaga
    12601 aatgtctgat ttttttgaag ctgttttgga aaatggtgca gatgccaagc aagcatctaa
    12661 ctggttgatg ggtgaagttt ctgcttactt gaacagtgag aaactggaat tagctgagac
    12721 aaaattgaca cctgagaact tagcaggaat gattcgtttg atcgccgatg gaacgatcag
    12781 ctcaaaaatc gccaaaaaag tcttccgtga attgatcaca aatggcggag aagcccaaga
    12841 agtagttgaa aaaaatggtt gggtgcaatt atctgatccg aacaaactac tgccaatcat
    12901 taatgaaatt ttggataaca accaacaatc tgtcgatgat ttcaaaaatg gaaaagatcg
    12961 tgcggttggc ttcctagtcg gtcaaatcat gaaagcaact aaaggacaag caaatcctgg
    13021 tgtcgtgaat aagctactta aagatgaatt agcgaaacga tagaggctga aaatgaaaaa
    13081 agcacgcgta atttataacc ccacctcggg aaaagaatta ttgaaaaaaa atttggcgga
    13141 tatcctacag gcattagaag aagcaggatt tgaagccagc gcctatgcaa ctacaccaga
    13201 accagattca gccaagaatg aagcatgtag agtcgcagaa ctaggttttg atctagtagt
    13261 ggcagctggt ggcgatggca caatcaacga agtggttaat ggaatcgctc cgcttgaaca
    13321 ccgtcctaaa atggccatca tcccagctgg tacaacgaac gactatgctc gagcattaaa
    13381 aattcctaga gataatatta aagcagcagc agaagtgata aaaaaacacc aaaccgtaaa
    13441 aatggacatc ggcctttctg ataacagtta cttcatcaat atcgctgcag gtggctattt
    13501 gacagaattg acttatgaag ttccatcaga actaaagagt atttttggtt atcttgctta
    13561 tcttgcgaaa ggagcagaga tgctccctcg agtaaagcca atcaaaatgc gcttgaagta
    13621 tgatgaaggg gaatatatcg gaaatgcttc gatgtttttc ctagggctga cgaactctgt
    13681 tggaggattc gaacagatcg cgccagatgc caaattggat gatgggaagt tttctttaat
    13741 cattgtgaag acagcaaata tctttgagat cttgcatatt gcagcactca tgctgaatgg
    13801 cggtcgacat gtcgatgatc caagaataat ctataccaaa acaagcagtt tgttcgttga
    13861 aacagaagat gatatcaatc gaccagtaat gatcaactta gatggtgaat acggcgggga
    13921 agcaccgatg catttccaaa atcttcatca acacattgag ttctttgcca ataccgatca
    13981 gattcctgat gaagcaatca caggtacaga tgaagaagaa ttagaggttg tcagcaaaga
    14041 attcgtaaaa gaagtcgaac gtttaacgga tgaagatatc gacggtgacg gaaaaatagc
    14101 agacgaaaca cctaatcaaa agaattaatc aaaagccggt gctgaacgta gtaaggtcag
    14161 tgccggtttt agttgtgtaa ttagctgatt tatagtagag ttcagtagga ttgaaaaata
    14221 ggcttaaagg agcaaaatat gacagaacag caaatagtaa agaaaaacga acgatataca
    14281 gtcgaaatcg tcgacttgag ttatgaagga atgggggtag caaaaatcga tggttatcca
    14341 ttatttatcg aaaatgcact ccctggagaa gaagtagaaa tactagtagt aaaagcaggc
    14401 aataagtttg gatatggaaa agtcgaaaca tttattacac aatcacctga tcgtcagcct
    14461 gtagaaaaca atgcattact tcgcataggt atcgcacctt tagcccattt gaagtatgaa
    14521 caacagcttg tcttcaaaca aaaacaagta gagcatgtaa tggcgaagat tgctaagatg
    14581 ccagaagttc ctgtacttcc aacggtcgga atggcagaac cattcggcta ccgtaataaa
    14641 gcacagatcc ctgttcgccg tatcgatgga cagttagcca ccggttttta caaaaagaat
    14701 agccatgatc tagtccctat cgaagatttt tatatccaag atcctgtcat cgatcaagca
    14761 atcaaagtag tcagagatat cttgcaacgg ttccaagtac gaggatataa tgaagaaaaa
    14821 aatgaaggac agcttcgtca catcattatc cgtcgtggtc attattccca tgaaatgatg
    14881 gtcgttttag ttactcgtaa agaaaaattc tttaaatcca aggaaattgc tgaagctatt
    14941 catgaagaat tgccagaagt ggtttcggtc atccaaaata tcaatgaaga aaaaacaaat
    15001 gtgattttag gagacaaaga aaaagtttta tatggcagag actatatcga agatcagcta
    15061 ttaggaaaaa cctatcgaat ctcagcaaag tcattttatc aagtaaatac tcaacaagca
    15121 gagtatctgt accaaacagc catcgagttt gccgagttag ataaaaaaga cattgtgatc
    15181 gacgcttatt caggaatagg tacgattggc ttgtctttag cagacagagt ggataaggtg
    15241 tatgggatgg aagttattcc agaagcaatt gaagatgcac agttcaatgc tttaaataat
    15301 aaaatcgaaa atgttcatta tgaagtcgga aaagcagaaa aagttatgaa aaaatggcaa
    15361 gaaaaaggga tcaaaccatc tgtgatcgta gttgaccctc cacgaaaagg attagatcat
    15421 cattttattg aatcagcaat cgaaatgtca ccagaacgaa tcgtctatat ctcatgtaat
    15481 ccggcaactt ttgcaagaga tgctaagtta tttgcagaag caggttatgc cgtgaagaaa
    15541 gttcagcctg tagatctttt cccgcaaacc catcatatcg aattagtcgc ttcattcgtt
    15601 aaaggagaat aaaaatggac caaaaagaaa aaatgaaaga attgatcaaa aagaaacaag
    15661 gtggaggccg atccaaacaa atggaaacac ctaagaatga tcgtaaaaac atgagaaaag
    15721 gccctaaaat ctttaataaa tagacgtgct aaagaaagac taagaagtgt taacttttgt
    15781 tattgtttct tagtcttttt agttagtcta cattattttg gcgatttgcc aataaggact
    15841 gttgagttgt gcaaaatcaa atctgggtca gaatgaacgt cattctgtct cagatttttc
    15901 gttatttcaa tttgattaac tgattgatca aaaagatttt tattttcata ttctcaaatg
    15961 attcaacact aaatatacat gtttgacgcg gaaattatta ttcgattttg gcggcatcag
    16021 gggggatgac gacgtagcag acccattatt ttactcaaga aaatgtccgt gcctattatt
    16081 tagaatacga tagtgaacgt gctgggagtt ttgttctttt agagaaagtc tctgaagata
    16141 aattagttgt cttagatttt gactgtaacg aaaaatggcg aaatggaaga gcaccagcaa
    16201 gtgattgacc ggatctacga agcgacacga tatctgcctt tggatcgttt gtgcttaagt
    16261 cctcaatgtg gatttgcttc aacagaagaa gaaaatgttt tgtttgaaga acaatggaaa
    16321 aaatttcttt tgtcaaaagc attgcagaag aagtttgggg aaaatcatta aatcatctag
    16381 gagagcttga taggaaataa cactatggta taatcagaat gcttaatact aactactgag
    16441 ggagccttat gaaaaagaaa attcttaaat ttatcggatg gttcttcgga atcattcttg
    16501 ggatcgcgtt tgcaatcttt cttgccttcc agctttcccc cagacctgga gcattcttga
    16561 tcaaccatat gttttcaaat actgttcaaa tcactgataa aaaaacgttc gatcaagcag
    16621 aaaaaaacgt ggcgaaaaaa acagatttga cgtatgagtc caagtataaa gagaatactt
    16681 atgacatcta ctatccaaaa aacagcaagg ggcctgttcc tgttctattt tgggtacatg
    16741 gtggaggatt cgtcggcgga gataaatcgg gtgtcaaaga atttgcgaca aaattagttt
    16801 cagacgccaa catcgcagtg gttgcgatga attatgaatt agcgcctgat tcagaatacc
    16861 ctaatcaagt tcttcaagtg aatgaactga tcaaagattt attagctgaa gaaaaagacg
    16921 acaaattgct agacatggaa caattatttt ttggtggaga tagtgcaggt tcacaaatcg
    16981 cattgcaata tgcagctata caaacgaatg atgcgtatgc taaacaaatg aaaatcaaac
    17041 agctacttcc aaaagatagt ctgaaaggaa ccatttctta ttgcggacct gttgatttga
    17101 agctaacagc acagcagcat tcggatgatc gatttatgaa gtttttcgtc aaaaccgttg
    17161 cgtggtcgtt gattggaaca aaagattgga aaacgagtga gcaattgaaa gaagctagtg
    17221 tggctgacca tgtttctaag gaatttccgc caacgtatat aactgacgga aatgcatact
    17281 cattccagga gcaaggtcaa gcattagaaa atcggttgac agaattaaag gtacccgtgc
    17341 aatccttgtt ttatacagat acaaaaaaag agattaccca tgaataccaa tttgattaca
    17401 cgctaaaaga atcgcaaaat tgttaccagc agacattgga ttttgtgaat aagtataagt
    17461 aagaggttgt gaaaaagccg tttcgttttg agtatgaaga aacgaagcgg cttttcacaa
    17521 cctctctttc aatacctttt tgttcttggg ataaaacttt ccctaacaat caatcgtgca
    17581 ggataagtca aaatgattgg ttcgtcccgt ataccgtcaa ttcgctccaa tgccattcga
    17641 acagctgctt gcccaattga tttcgcattg atttgaacac tggtcaagcc aggggagagg
    17701 tttgcgattg tctcgatatt atcaaagctt gctacaacaa tctcttctcc aatttggata
    17761 tttttttgtt tcaatgcccg atagacacca attgccattg gatcgctccc gacaagaatg
    17821 gcagtaggaa gaggggattc attttctaat agattagtcg caagtacctc accttgtttt
    17881 tctgtccagc cacctagatg ataagacata tatttttcta agtgatgatc gatcataaaa
    17941 tgctgatagg cacgcatcct cttttcattt atagaaatct gcttcccacc gaattcatcc
    18001 atatcgactt tatatccccc gatataggct attcttttat gaccattttc taagaacaat
    18061 tccagtacgg ttttagtcat ccgttcaaga tctgcataca ccatatcgac ctcttgctgg
    18121 atgtcagggt tatccacgac gactatttgc atgttttgtt ttaacaaggc ggtaatcgat
    18181 tctttcgtaa cagtcccaac aacgatcacc gcacctaact gatctagatc ttcccattgt
    18241 ttaggattat cagatagatt atagattcga ttcatcccta aatgcagacg tcgagcttct
    18301 tcttccatcc ctactcgcaa atgtttgtaa tagatatcct gttcttcgct cgctttgccg
    18361 atcgtagaga tcacaccaat cgaaggcatt ctctttttga tatatttacg agtactgatg
    18421 tcataatcca acgcagcagc tgcctctact atccgttgcc gagtagtctg agccacatgg
    18481 atcgtctgat cgttattcaa cacacgggaa actgtagcag gagagacatt tgcctttcgt
    18541 gcgacatctc taattccaac catttttaca cactcctcaa aaaaataaaa aatttttatt
    18601 gacaaaattg tttagtaaac agtatacagg aattataaaa aaaagtaaat ggtttccgtt
    18661 ttgttgggag tgaaatgatg gatattacgc atagaaaaac agataaaaat ttcatcttat
    18721 ttgatgaaaa gagccgagtc ttccatttac gaaatacatt tctttcttat ctgataaaga
    18781 tagaagaaag caatgtattg gcgcatattt attttggcaa accagtcaag cagtacaaag
    18841 acaataaaaa ttatccccga agagatcgtg gattttcagg aaatgttccc ttaaatcctg
    18901 accggtctct gtcgaaagat acactaccgc aagaatactc ttctcatggc agcatggact
    18961 ttcgaacgcc cgcatcgatc attcagcgca agaatggatc agacttactg gatctgagat
    19021 atgacagcca ttatattaca gatgggaagc cagagatcga aggcttgcct caaacttatg
    19081 tgatggacaa gtcagaggca caaacgttgg tgatttcact taaggataga gaaacagcga
    19141 tttatttcga tttgttctat accatattta ctgatagagc agtgatcaca cgatctgtaa
    19201 agataagaaa tgaaaccgga gaaacaatca aactagaaaa agcggctagt ttccagctag
    19261 actttgccca tactaggcga tttgatgaag tcattgcttt gccaggagca catgtcaatg
    19321 aaagacagat ttcacggcaa tctgttttat ctggaacaaa agtatttgaa agccgtcgag
    19381 gaacgagcag tcaccatatg aataacttta ttgctttagt tcatcatcat acgacagaaa
    19441 atactggaga agcgatcgga cttcaatttg tttattccgg caaccattct ttcgaacttg
    19501 aaaaagatca gatcaatcaa ttgagagtcg tcggtgggat caacagccat cgtttttcgt
    19561 gggaattgaa tgcaggccag tcttttcaaa cacctgaaat gatcctttct tactcaagtc
    19621 agggattgaa caaaatgagt cagatccacc atgagttgct acgtgaaaga atcgcaagag
    19681 gtcgtcatca atttgctgaa cggccgattc tggtcaataa ctgggaagcc acttactttg
    19741 acttcaatag tgaaaaaatc aaggcgatca ttgatgaagc aaaggagcta ggcatcgaaa
    19801 tgttcgtatt agatgatggc tggtttggaa aaagagatgc ggacaattct tcattaggag
    19861 actggttcga gtatgaaggc aagttgacaa atggattacg tgaaatagca gattatgctc
    19921 atagcaaagg attgaaattt ggtttatggt ttgaacctga aatgatctcg gttgattctg
    19981 aactgtatcg aacacatcct gattttctta tgcaggagcc aggtagaatg ccttctgctt
    20041 ccagatcaca acatgtactg gattttacaa ggttagatgt tcggcagacg attgagaaac
    20101 aaatgagaaa gattttagat acgattcctc ttgactacat caaatgggat atgaaccgtt
    20161 cgctttcaga tgtctattcg attacccttg acccacagag acaaggagaa gtagcgcatc
    20221 gttatatgct cgggctttat gaactacttg aacatttgac aactgattat ccggaaatct
    20281 tatgggaagg ctgttctggt ggaggtggac ggtttgatgc cgggtttatt tattatatgc
    20341 cgcagtcatg gacaagtgat aacacggatg cagtggagcg tatgaaaatc caatatggaa
    20401 caagccttgc ttatccgatt tcgtccatca cagctcatgt atcagctgta ccaaatcatc
    20461 agaccgacag atctacttcg ttgaaaacaa gaggagaaac agctatgagc gctgtttttg
    20521 gttatgaact ggatttgaca aagctgtctc ctgaagaaaa aaagcaagta aaagaccaaa
    20581 ttatatccta tcaaacgatt cgtccggtga tccagtatgg ccattattat cgtttggcga
    20641 gtccttttga agaaaatatt gccgcatgga tgtttgtgtc tcctaaacaa gatgaagcta
    20701 ttgtgttttt agggaggata ctagcttcag ctcaaccagc gttccatgaa gtatatctga
    20761 tggggttaga tgatgaggca ctttatcagg aacagacctc gaagcggata ttttcggggg
    20821 ccgaattgat gacagttgga ctttacttcc ccgattttca aggtgatttc caaacagaac
    20881 tgcttcattt caaaaagtta tgagagagaa ggaaaaaagt atgaaagcaa atataaaatg
    20941 gttagatgat ccacaagttt ttagagtgaa ccaatcaaat gcacatagcg atcatgtttg
    21001 ttatgcaaca agggaagaag tagaaaccac atcctccctt aaacagtctt taaatggatc
    21061 atggcgtttt tgttattcgc caaatccttc ggtacgacca aaaacatttt ataaaaaaga
    21121 ttttgatgcc tgttcttttg atgaaatcac tgttcctagt catattgaat tagcaaatta
    21181 tgacaagatc cattacatca atacgatgta tccgtgggag ggacatgtat ttcgccgtcc
    21241 ccctttcacg acaagccctg agaaagaggc ggaagggatc ttttcagctg ctaaagacaa
    21301 tccggttggt tcgtatttga cgacttttcg attagaagaa ggtctaaagg gaaaacaaat
    21361 ctcgattcat tttgctggtg tagaacaagc aatgtttatt tggttgaatg gtcattttat
    21421 cgggtatgcc gaagattcgt tcactccgtc tgaatttgat ttaacacctt atttggaaga
    21481 agaaaatatt ctagctgtgg aggtttacaa aagaagtaca gctgcattct tggaagatca
    21541 ggactttttc cgattttttg ggatcttcag agatgtcttc ttagttgctc gaggacaaaa
    21601 ccatttagaa gatctttgga tcaaacctcg cttagatgac aaatgtcaaa atggcaaact
    21661 tgaagtagat ttgtcttttg agcaagtagc caaaaacagt cagctgaatg tagcgatact
    21721 agatcctgat gggaaggaaa tctatcaaca gcaatatccg ttaaaagagc aaatgacgat
    21781 tcgcatcgat ggatttgaaa atgtggcact gtgggggcat tttcagccca atttatataa
    21841 gcttgaattg gagacgatag acgaagaaaa aagagtaacg gagtatattg actattcctt
    21901 cggttttcgt aaaatcgaaa tcaaagataa aatcatctac ttcaatggaa aacggatgat
    21961 tatcaacggg gtaaatcgac atgagtggca tccgaaaaaa ggacgcgcca tcactataga
    22021 agatatggaa aaagatttgg ctatcataaa aaatgcaaag atcaacggcg tacggacatc
    22081 tcattacccc aaccagatca cttggtatta tttatgcgac caagcaggaa tccatgtgat
    22141 ggcggaaacg aacctcgaat cccatggctc atggcaaaaa atgggcgcat ttgagccttc
    22201 ttacaacgta ccaggtgcag tacctcagtg gcaggcggct gttcttgatc gagcaaaaac
    22261 gaatttcgaa acattaaaaa accatccaag tattcttttt tggtctcttg gaaatgaatc
    22321 ttatgcggaa gaaaacattc gacaaatgaa cgattacttc aaaaaagtag accccgatcg
    22381 acttgtccat tatgaaggga attttccaaa tccagcattc gaagattcga tttcagatgt
    22441 aaaaagctgc atgtatgcgc caccagaaga gatcattgac taccttgaaa ataaagcgga
    22501 aaaaccgttc atcttatgtg aatatatgca tgacatgggg aattcgttag gtggaatgga
    22561 ttcttacatg acgttgcttg atcgttatga aatgtaccaa ggaggctata tatgggattt
    22621 taaagatcag gcgatccaag tgactgatcc aatcacaggc agagaagtga tgcgctatgg
    22681 cggtgacttt gacgataaac caagtgacta tgaattctca gggaatggga tcgtttttgc
    22741 agatggacaa gaaaaacccg ccatgcagga ggtaagatat tattatgaaa aatacagtaa
    22801 ataaaagtca tatggatacg gaaaaagttg caatcgtctt cggcgactgt acattaggtg
    22861 tcaaatcggg gaatacgcat tatatttttt cttatacaag aggcggactg gaatcgctca
    22921 ataaaaacgg aaaagaatgg ctataccggg aaacacttcc agttttttgg cgagcaacga
    22981 ccgataatga cagaggtagc ggatttggat ttaaatccgg tatgtggtta ggagccggat
    23041 tgtatccaaa agtcaaagaa atagaagtac tggtggacaa taaaaagatc gatttgccga
    23101 ttgctccagt aaataatcaa tattcaagta atgaatacgc tgaaacggtt gaaatcattt
    23161 ttacactgga aatcaacaca gtaccaaaaa cccaagtgac aatttcctac caaatcgctg
    23221 gagagggtaa aatgcttgtc aatatgcatt attatggaca aaaaggatta ccggaattac
    23281 ccgcattggg tttaaggttc attatgccaa cgaaagcaaa attttttgag tatgaaggcc
    23341 tatcagggga aacatatccg gatcggatgg ctggtggtgt cgaaggtatt tataaagtgg
    23401 caggactccc cgtttcccca taccttgttc cccaagaatg cggcatgcat atggaaacga
    23461 aatgggtaga actaacaaga aatcaaacgt tggataatac ggataaagat caagaagatt
    23521 tttcgttgcg atttgaacag caggatacgc catttgcttt ttccgcttta ccattcaaag
    23581 cagaagaact ggaacaggca acacatatag aagaactacc gccagaaaga cgaacggttc
    23641 ttacgatttt tgcaaaagta agaggagtcg ggggtatcaa tagctgggga gcggatgttg
    23701 agtcacctta ccgaatcagc agcgaagagg accatgaaat cggctttatt attcagtagc
    23761 aatctttaga ttattatttg aaaaacggag tttacaagac aaagctgctt tattctatat
    23821 gtcatgaaca agagagtaaa aaagttctca gatacttttg ttcatg
//
LOCUS       DACDKM010000029        23830 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20834_2016
            SAMN12640370-rid9891663.denovo.029, whole genome shotgun sequence.
ACCESSION   DACDKM010000029 DACDKM010000000
VERSION     DACDKM010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640370
            Sequence Read Archive: SRR10040891
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23830)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23830)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742835.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742835.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:04:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,012
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 29 of 75
            Pseudo Genes (incomplete)         :: 33 of 75
            Pseudo Genes (internal stop)      :: 19 of 75
            Pseudo Genes (multiple problems)  :: 5 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23830
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640370-rid9891663.denovo.029"
                     /isolate="E20834_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(54..788)
                     /locus_tag="IV334_001146"
     CDS             complement(54..788)
                     /locus_tag="IV334_001146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin family protein"
                     /protein_id="HAP8943233.1"
                     /translation="MDYSLMTRLAAEMIGTALLIVLGNGAVANVDLKGTKGYGSDWML
                     IAVGYGFGVMMPAMIFGGISGNHINPAFTIALASNGLFPWAEVVPYIIAQLIGAAIGQ
                     LIVVACYKPYYDQTEDVNHVLGTFSTINSVGSKFNGFVNEFFGSFVLFFGALGITKAP
                     FFQDNLGTAHLALGFLVWTLVASLGGPTGPGLNPARDFAPRLVHALLPLKHKGSSQWG
                     YAWVPVVAPICAGVVAVFLYKLLFMA"
     gene            1115..1525
                     /locus_tag="IV334_001147"
     CDS             1115..1525
                     /locus_tag="IV334_001147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidA/LrgA family protein"
                     /protein_id="HAP8943234.1"
                     /translation="MKFFQEFFIIIFFSVLGEGIRIFAHLPIPGSILGILFLFLAFEM
                     HLVDPEKIGATGDFLLNNLAILFVPAGVGLLEYFDDIASIWPVLLGAVVVCSIVTMVA
                     AGKTAEGVEALLGYVRKKKAERVSVNEMEENQID"
     gene            1518..2219
                     /locus_tag="IV334_001148"
     CDS             1518..2219
                     /locus_tag="IV334_001148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LrgB family protein"
                     /protein_id="HAP8943235.1"
                     /translation="MTEFFVNPLFGLSLTLGVYVLSDKFLRKYKLPVLNPLVLSIVVI
                     ILFLHFSGISYKDYNIGGNFLTMLITPATVALAIPLYKNFRLLKENFYPVMAAILAGI
                     VANCLVSVGFGYFFSLHKEMVISLLPKSVTTAISVDLSHTMGGVNAVTLAIVVSTGIF
                     GSLIATHIFRLFKIESPVARGVALGSTSHAIGTAKAIEIGEIEGIISGLAICVNGILT
                     VLLLPLFFQPFAGLF"
     gene            2219..3805
                     /locus_tag="IV334_001149"
     CDS             2219..3805
                     /locus_tag="IV334_001149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M protein trans-acting positive regulator"
                     /protein_id="HAP8943236.1"
                     /translation="MRKPLFYATITKTFEHKKGGKDVYLSSEYLKQFDKDLYYKILLL
                     ESFEDQAWHTAAQLAQVVQLDARSVSKYLNELSKNYQQFSGKTHPLFTKNHRSGYNFY
                     DTLDSIEHERFLIYLVQSTLKFQLLHDIFFEEFHTMYQFAQKHYISESTAHRKINEWK
                     QQLQTYGIRLQRGTYIAQGEEEIIRLYLHMTFWQLFRGKIWPFETISQMDVKNMAEHI
                     MAFFNVRLNEIKKRRLEYMLGAFFLRKSQKHYVVLNEKKRRLISDNLLFQRFCQVMEP
                     VFPNYFQVEDELGALFLVLMTREEYYSDPKIRKKIFDFHQQAKTPPFTALSEAKAALS
                     LYQEEQGLPAENLTFEAENYLFSSHFFAYLFPNAKETIDGNSSDFINHLVIENKELKQ
                     WLVHFFESRHKHPNHLAFKNHAFLMGRYLTVFKTLGAFTPQLPKITILLMTDFPLFEE
                     QLLEEGLRTFFRNEYQLIFLPTDYRGREVDLLISTSKVHRKPWADLDYFIVTEELKLI
                     DYIQLSQKFEMIQKQKLLKQ"
     gene            3824..4045
                     /locus_tag="IV334_001150"
     CDS             3824..4045
                     /locus_tag="IV334_001150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016186310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2829 domain-containing protein"
                     /protein_id="HAP8943237.1"
                     /translation="MTFEEILPELKKGAKIIRKGWSGFELYVVLVSDDEFDGCPVTPY
                     LLIKTSDEGFSSFAPTVCDILADDWAIVE"
     gene            4042..4803
                     /locus_tag="IV334_001151"
     CDS             4042..4803
                     /locus_tag="IV334_001151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase"
                     /protein_id="HAP8943238.1"
                     /translation="MKNQYPDLAKQVVFITGAASGIGAAQARAFLEQDAFVFGLDQKF
                     GKMEEISLRFPETFAYALGDVRQMKELQQAVSKCQNHFGEVTILLNTAGILDAYKPLM
                     ETDESLWDLIYETNVKSMYQLTKIILPDMIRQKSGTIINMASIAGLIAGGGGIAYTSA
                     KHAIVGFTKQLALDYAGQGIRVKGIAPGAIQTPMNAADFEGKGEMAEWVANETPVKRW
                     AQPEEVAELTLFLASPQASYIQGTIIPIDGGWLLK"
     gene            5183..5692
                     /locus_tag="IV334_001152"
     CDS             5183..5692
                     /locus_tag="IV334_001152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HAP8943239.1"
                     /translation="MEKNNTRSAKRWTTTDVTRMALVTGLYVAVTLVLSVFSFGVIQI
                     RLSEMFNYLSLYNKRYIVAVTAGVALANIASPLGLIDVAVGSVSTLLVLLINYKITSR
                     IKNMKIKMAVTACVFAFSMFTVAGQLTILYQLPFFLNWWVIALGELASMIVGGIIIYW
                     IGKKVDLSK"
     gene            complement(5767..6306)
                     /locus_tag="IV334_001153"
     CDS             complement(5767..6306)
                     /locus_tag="IV334_001153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter BioY"
                     /protein_id="HAP8943240.1"
                     /translation="MNRTLKELILAAEFAAIISVLSQMTIPFGLVPLTGQTFAIGLTA
                     TFLGRKLGLISVGVYLLLGLIGLPVFSGMTGGIAVLFGPTGGYLIGFLLQTWITGWMI
                     ERTDAHYLYAIFANLMGSLAALICGTIWLKISGDLTFTTAFASGLLPFLLPEAIKATG
                     AAYIGILMKKRLNRFLHTN"
     gene            complement(6446..7057)
                     /gene="rpsD"
                     /locus_tag="IV334_001154"
     CDS             complement(6446..7057)
                     /gene="rpsD"
                     /locus_tag="IV334_001154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010766410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HAP8943241.1"
                     /translation="MSRYTGPSWKISRRLGISLSGTGKELARRPYAPGQHGPNSRGKQ
                     SEYGMQLTEKQKLRHMYGMNERQFRNLFVKASKIKEGKHGVNFMILLEQRLDNVVYRL
                     GLATTRRQARQLVNHGHILVDGKRVDIPSYHVEVGQVISVREKSQNIVTVKEAVEATV
                     GRPAFVSFDAEKLEGSLTRLPERDELYPEIDEALVVEYYNQSL"
     gene            7373..8170
                     /locus_tag="IV334_001155"
     CDS             7373..8170
                     /locus_tag="IV334_001155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate-nitrite transporter"
                     /protein_id="HAP8943242.1"
                     /translation="MNPVSPLFEKIDASIEKKANLFENSFSRYAVRAVLACLFLTLGT
                     AVAFAIAMKGEDIAHGLGKILYAFMFSWGLVMILYMNAELGTSNMLYMTVGVFRQRLA
                     LSKAAKILFACIFFNMIGGILCGFLISMTTPFQDLTLDNFMLESIAVKLAKPSATILV
                     EGMFANIVVNTAVLISMRMKEDVGKVITVVFIIFIFAFLGYEHVIANFPAFTLTYFAS
                     HGQMDGMTVSNVLHNLFFALAGNYIGGGLVMGLGYAWLNQSKSSYVD"
     gene            8198..8833
                     /locus_tag="IV334_001156"
     CDS             8198..8833
                     /locus_tag="IV334_001156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HAP8943243.1"
                     /translation="MEVLVFPKNEEKALQMEKYMKNLFSFAGVPKPERALLQQPLLDE
                     SKNLSISEVLAFVHYYYEQIEREYQYVAIDLVQKNIKRLTFEDLVVLLPLVQKKEWWD
                     SIDSWRKVYSTWCKAYPEQREEVFQLFEKKEDFWLRRIAITLQLGYKQQTDTGLLIRA
                     IDEDRQTKEFFIQKAIGWALRDYSKTDADWVKRQLTTDLSKLAVREASKYL"
     gene            8853..9626
                     /locus_tag="IV334_001157"
     CDS             8853..9626
                     /locus_tag="IV334_001157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAP8943244.1"
                     /translation="MRRKLFAFDIDGTLLDSNKQALDSTREALEKLRKAGHLVTIATG
                     RSRFHAQEIIRDLDFTNYILCNGAAGFLDHEQVYKNLLDRDQLDAFVQEAHHEQIDTA
                     FVSLDNIKRSSSNDVTVMEEAMHSFGAVLPELDQFFAEEQEIYQALAFFDQTYDGKFS
                     SYDKIRFVRWHENSVDVVPKNGSKAATILHLAEQVGIPHEDIISFGDGQNDREMLRMS
                     GIGIAMGNAVPEVQAEAKMVTDTNDQDGIWKALKELSVI"
     gene            10034..10831
                     /locus_tag="IV334_001158"
     CDS             10034..10831
                     /locus_tag="IV334_001158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003128088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00282 family metallophosphoesterase"
                     /protein_id="HAP8943245.1"
                     /translation="MRVLFIGDVVGSMGREMITEYLPRLKKKYRPQVTIVNGENAASG
                     RGITEKIYKKFLQDGVDVVTMGNHTWDNRGIFEFVNEAKKMVRPANFPEGTPGQGMVF
                     VKVNQVELAVINMQARSFMVDLDDPFRKMNELVEEARKRTPIIFVDFHGETTSEKQAM
                     GWFLDGKVSAVVGTHTHVQTNDARILPRGTAYLTDVGMTGPYDGILGMRREPVIEKFL
                     TALPKRFEVVEQGRSILSGCILELDDTTGHAKEIQLIQINDDRPFME"
     gene            10809..11222
                     /locus_tag="IV334_001159"
     CDS             10809..11222
                     /locus_tag="IV334_001159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8943246.1"
                     /translation="MIDRSWSRKRGSFLQQEPEIQKEVRKLTKLLRENETIIRYKELE
                     EKIQQNQYLAELREKIKQAQKDAVHFAHYDKPAAEKEAIKQADQFMQEFDQHPLVVAY
                     RKQLLEADDLLHHLTTMIQEEINGQIEEEKHASKN"
     gene            11206..13851
                     /gene="mutS"
                     /locus_tag="IV334_001160"
     CDS             11206..13851
                     /gene="mutS"
                     /locus_tag="IV334_001160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HAP8943247.1"
                     /translation="MPQKTKNTPMMEQYLSIKAQYQDAFLFYRLGDFYELFYEDAIKA
                     SQLLELTLTSRNRNAEEPIPMCGVPHHAAQGYIDALIEKGYKVAICEQVEDPKTTKGM
                     VKREVIQLVTPGTVMDSKGLSAKDNNFLTAVVSQGNEFGFAYADLSTGELKTARLHDE
                     EAVLNEASALQTKELVLGSEITDTLREQLSGRLGLVFSQQNEMEENAEFSFLTSELVD
                     PLEKEATGKLLTYLSVTQKRSLAHIQKAVEYQPDHFLKMDYYSKFNLELSQSIRTGKK
                     HGTLLWLLDETKTAMGGRLLKQWLDRPLIQAKQIKARQEMVASLLDSYFERIDLQSAL
                     TKVYDLERLAGRVAFGNVNGRDLIQLKTSLEQVPMIRGLIEGIDQGQWQSLLSEMQPM
                     DHLVQLIDQAIQDDPPLQITEGNIIKDGFNEQLDMYRSAMRNGKKWLAELEAKERQET
                     GIKNLKIGFNRVFGYYIEITKANLGNAELEKYERKQTLANAERFITPELKELETQILE
                     AEEKSVDLEYQLFLAVREEVKKAIQPLQVLAKAISAVDVLQSFATISERYQYVRPELV
                     SDKHQLLIKDGRHPVVEKVLGHQEYIPNSVEMAEDEMILLITGPNMSGKSTYMRQLAL
                     TVLMAQMGCFVPAKQAVLPIFDRIFTRIGASDDLIAGQSTFMVEMMEANQALRYATPN
                     SLILFDELGRGTATYDGMALAQAIIEYIHRHVQAKTLFSTHYHELTVLEKELPQLKNV
                     HVGAVEKDGEVVFLHKMMDGPADKSYGIHVAKIAGLPSNLLERAADILHTLENGDNGH
                     HTVPAEVEEKTKTAKQEQIKPPAEKVNQDHIQDRVKEENQQLSLFSELSENETEVIES
                     LKQINLLEMTPMDALNTLYELQKQL"
     gene            13869..15977
                     /gene="mutL"
                     /locus_tag="IV334_001161"
     CDS             13869..15977
                     /gene="mutL"
                     /locus_tag="IV334_001161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HAP8943248.1"
                     /translation="MAKIQELSERLANQIAAGEVVERPASVVKELVENAIDAGSTQID
                     ILVEEAGLRTIQVIDNGEGILADDVENAFKRHATSKIHNRDDLFRIRTLGFRGEALPS
                     IASVSEMTLETANQEEKQGTYLSLKGGEVIEHRPAPLRQGTKITVSNLFFNTPARLKY
                     VKTLHTELANIGDIVNRLALSHPSIAFRLVHDGNKMLATAGNGDLKQTIAGIYGLSTA
                     KKMLKIEGKDLDFELFGYVSLPEVTRASRNYLSTIINGRFIKNFSLNKAIVAGYGSKL
                     MVGRFPIAVLEIKMDPLLVDVNVHPTKQEVRLSKEKELTELISNAIQKALSQENLIPS
                     AADNLRFKKKVPDQPKTEQIEIDLSYAFEEEKPKKPSSLNFDRETGTFFVEELKNTFV
                     DRIVDKSVENVENYSGFSEENTTYPEKTAKTVDKFGENVDNFVEKTVEKSEKKEFPIV
                     DNMEKTNEEQADKSVEEDEKIYEEELTLHPEFDFTQGNDKTLEQAIKKLETEKPVQRF
                     PQLEYFGQMHGTYLFAQSSDGLYIIDQHAAQERIKYEYFREKIGEVSDDLQELLVPFV
                     LDYPNSDALKLKEQKHLLEDAGIYLEEFGQNSFIVRAHPTWYPNGEEESIIREMIDML
                     LTTGSVSVKKFREATAIMMSCKRSIKANHYLNEAQARVLLKDLARCENPFNCPHGRPV
                     LIHFTNSDMERMFKRIQDPH"
     gene            15999..16559
                     /locus_tag="IV334_001162"
     CDS             15999..16559
                     /locus_tag="IV334_001162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation inhibitor Maf"
                     /protein_id="HAP8943249.1"
                     /translation="MKIVLASQSPRRKELLAHIVSEFEIEPADIDETPFPDEQPVDYV
                     RRMAEEKARTVWEQDEKEDHLVIASDTTVVLNQEIMGKPEDLAEAEFMLKKLSGETHT
                     VYTAVVLKTKEREERILAEAHVTFYPLTDEEIKRYLETGDYADKAGAYGIQNAASVFV
                     KEISGDYYSIVGFPIGAVNQALKTFI"
     gene            complement(16644..18632)
                     /gene="treP"
                     /locus_tag="IV334_001163"
     CDS             complement(16644..18632)
                     /gene="treP"
                     /locus_tag="IV334_001163"
                     /EC_number="2.7.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system trehalose-specific EIIBC component"
                     /protein_id="HAP8943250.1"
                     /translation="MAKYQADAEKLLHDIGGKENIAAVSHCATRMRFVLNDPGKADEK
                     AIEDIPSVKGMFTNAGQFQVIIGNDVSTFYNDFVAVSGVEGVSKEQGKAAAKQNLHPV
                     QRAIAVLAEIFTPLIPAIIVGGLILGFRNVLEGIQFESLGGTIVEHSKFWNGVNAFLW
                     LPGEAIFHFLPVGITWSIAKKMGTTQILGIVLGITLVSPQLLNAYSVASTAAADIPFW
                     DFGFAQVQMIGYQAQVIPAMLAGFMLAYLEIFFRKYIPQSISMIFVPLFSLLPTVLAA
                     HVILGPIGWTVGSWISNIVNTGLTSSISWLFSAVFGFLYAPLVITGLHHMTNAIDMQL
                     IADFGSTNLWPMIALSNIAQGSAVLAIIFLHRGNKKEEQISIPAMISCYLGVTEPAMF
                     GINLKYVYPFVAAMVGSGLAGMFANLMGVRANAIGVGGLPGILAIQAETWVPFIIAMI
                     IAVIIPFGLTIIFRRQGILNKIDPAVPENAADVQLQTANGATATPQSFEPVSATGTAV
                     ATKETLFAVAAGTIKEITEVNDPVFSQKMMGDGYAVEPSNGKVYAPVNGKVTSVFETK
                     HAIGILSNEGLEVLVHMGLDTVELKGVPFNVFVKEGYLVTPETLIAEMDLPEIEQAGK
                     KTDIIVALTNNEKVAGLSLDQSGLVRPGEAVGKAEVKS"
     gene            18855..19568
                     /gene="treR"
                     /locus_tag="IV334_001164"
     CDS             18855..19568
                     /gene="treR"
                     /locus_tag="IV334_001164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose operon repressor"
                     /protein_id="HAP8943251.1"
                     /translation="MNKFNEIFLDLEQKILAKEYPPHSLLPSENQLIKIYGVSRETIR
                     KALNLLTTEGYIQKKQGKGSIVLDRNRFDFPISGLTSYKELQETQRIPSETIIHTLEE
                     TEVTKALNEITGWEIGAPVWHLIRERKINGEVVILDTDYLLKEIVPILTPAQAQGSIY
                     EYFENELSLTIAYAQKEITVEEVTEIMKTAMDLHDDTYAVVVRSLVHLEDTRCFEYSE
                     SVHRLDKFRFVEFARRRKV"
     gene            19645..20091
                     /gene="msrB"
                     /locus_tag="IV334_001165"
     CDS             19645..20091
                     /gene="msrB"
                     /locus_tag="IV334_001165"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HAP8943252.1"
                     /translation="MDKPIRPEKEELKEKLSPMAYAVTQENATERPFSGKYDDFYEKG
                     IYVDIVSGEPLFSSAEKYDAGCGWPSFSKPITKRQVREKADFSHGMHRIEVRSKEADS
                     HLGHVFTDGPVDQGGLRYCINSASLKFIPYEKMDELGYGEFKSLVE"
     gene            20261..20863
                     /gene="ruvA"
                     /locus_tag="IV334_001166"
     CDS             20261..20863
                     /gene="ruvA"
                     /locus_tag="IV334_001166"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HAP8943253.1"
                     /translation="MYEYLTGVVTFINPYYIVVETNGIGYQIALGNPYRYSSKLNQKI
                     KIYVHQVIREDAHLLYGFDSLEEKQLFLRLVSVSGIGPKSALAIMASEDHSGLIQAVE
                     SSDVTYLTKFPGVGKKTAQQMILDLKGKFGELSIDTPFSLFDEANTKDATALSEAIEA
                     LSALGYSDREIKRVEKQLKEVDNQTTDEYLRQALKLMMKK"
     gene            20876..21877
                     /gene="ruvB"
                     /locus_tag="IV334_001167"
     CDS             20876..21877
                     /gene="ruvB"
                     /locus_tag="IV334_001167"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HAP8943254.1"
                     /translation="MTDEYLLSPESGENELSLEKSLRPQLLSQYIGQRKVKQELQIYI
                     EAARNREEPLDHVLLYGPPGLGKTTMAMVIANEMTVNIRTTSGPAIERPGDLVAILNE
                     LEAGDVLFIDEIHRLPRVVEEMLYSAMEDFYVDIMVGQGPTAHPVHFPLPPFTLIGAT
                     TRAGMLSAPLRDRFGIISHMEYYDEQDLQEIVLRSSDIFQTEIIEEGALEIARRSRGT
                     PRIANRLLKRVRDFAQVQGDGKIDRPIADQALTLLQVDQAGLDYVDQKLLRTMIELYG
                     GGPVGLSTLSVNIGEERETVEDMYEPYLIQKGFLKRTPRGRIATAYAYEHFGYDYHI"
     gene            21906..22484
                     /locus_tag="IV334_001168"
     CDS             21906..22484
                     /locus_tag="IV334_001168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HAP8943255.1"
                     /translation="MSDTTRTKQLIKNVLIDLCQTQSFRKISVQKIANEAKINRQTFY
                     YHFTDKYDLLRWVYCEDALHYLDPQCLSLDNWEEQALKMLKAIAEKKEFYANTVLSDP
                     EILQKEFSQLIQKSFIKIFEQMDEERQLSARDKLFYARFFSYGCSGVLVNWITNEYEE
                     TPMEIAMQLFRLAKDTELYSYRLYAKEELEEQ"
     gene            22536..23018
                     /locus_tag="IV334_001169"
     CDS             22536..23018
                     /locus_tag="IV334_001169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight phosphotyrosine protein
                     phosphatase"
                     /protein_id="HAP8943256.1"
                     /translation="MTKILFVCLGNICRSPMAEGLLRKRIALEGREAEFFVDSAATST
                     YEVGKTPHPGTKKILNQEKVDMTNMVARQITPHDFETFDWIIGMDQENVEELKRRAPR
                     SAQGKIHLFLSSIPGKEKENVPDPYYTNNFDETYHLITEGLNHWWNIWIDENKITNNL
                     "
     gene            23135..23509
                     /locus_tag="IV334_001170"
     CDS             23135..23509
                     /locus_tag="IV334_001170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8943257.1"
                     /translation="MNEETVSPMKKLTKLVTHTSYQLFDSFVNDTEEHAKNVRLQGKF
                     ILELQKAATEHSLVILQVRANRLSSKYETISGWVATKTVEKDRVMIRLQNEEQQIRMV
                     PVEQIEKITILSPAGEQERLSK"
BASE COUNT         7782 a         4285 c         5114 g         6649 t
ORIGIN      
        1 taaaaaaact tggtacgcaa tcgctgtacc aagttttttt atttagcttt tgattaagcc
       61 ataaataata atttatataa gaatactgct acgacaccag cacagatagg tgctacaact
      121 ggtacccatg catatcccca ttgagaagaa cctttatgtt tcaatggaag aagtgcgtgt
      181 acaagacgtg gagcaaagtc gcgggctggg tttagtccag gacctgtagg accaccaagt
      241 gaagcaacta atgtccatac aaggaaacca agtgctaagt gagctgtacc aagattatct
      301 tggaagaatg gtgcttttgt aatacctagg gcaccaaaga ataaaacgaa tgaaccaaag
      361 aattcattga cgaaaccatt gaatttagaa ccaacgctgt tgattgttga gaatgtacct
      421 aacacatggt ttacgtcttc tgtttggtcg tagtaaggtt tgtaacaagc tactacgatc
      481 aattgtccaa tcgctgcacc aatcaattgt gcaatgatat aaggaacaac ttcagcccaa
      541 gggaatagac cattagatgc taaagcaatt gtaaatgctg ggttgatgtg gttaccgctg
      601 attccaccaa aaatcattgc tggcatcata accccgaatc catacccgac agcaatcagc
      661 atccagtcac tgccatatcc ttttgtacct tttaaatcta cgtttgctac agcaccgtta
      721 cctagaacga ttagtaatgc tgtaccaatc atttccgccg caagacgggt cattagtgag
      781 taatccataa atacctccaa aagttataaa aatgcaaaaa aaaaagaaac atcaacacga
      841 aaagcatcgg tgcagtgttt cgtgtcggaa ggatttctgc agtcttaaca aatgtgaata
      901 aaaaaatgaa ttccattact tataagaaac cagaccaaca gtaaaactcc aactcttttt
      961 aaaagttctc cacaaatcac caagcttaac tataaatata ccaaaaaaaa aacgtttttt
     1021 ttctcaaaaa ttgtatctta gaaacagcat gttttgaaac tttcagaaaa ggtttgttag
     1081 taaaatgaaa gctatacgta aggagtgttg tttgatgaaa ttttttcaag agtttttcat
     1141 tattattttc ttttctgtat taggagaagg catacggatt tttgctcatt tacctattcc
     1201 tggaagtatc ttaggtattc tttttttatt tttagcattt gaaatgcatc ttgttgatcc
     1261 tgaaaaaatc ggagcaactg gtgatttttt attgaataat ctagccattt tatttgttcc
     1321 tgctggtgta ggtttattgg aatattttga tgatattgct tcgatatggc ctgttttact
     1381 gggagcagtc gtcgtgtgtt ccatcgtcac gatggttgca gcgggaaaaa cggcagaagg
     1441 tgtagaagct ttgctgggct atgtccgtaa gaaaaaagca gaaagggtat ctgtaaacga
     1501 aatggaggag aatcaaattg actgagtttt ttgtgaatcc attatttggc ttaagtttga
     1561 ctttaggagt gtacgtatta tctgacaaat ttttgcggaa atataaactg cctgttttga
     1621 atcctctcgt attatcgatt gttgttatta tcttattttt gcatttttct gggatcagct
     1681 ataaagacta taatatcggg ggcaattttt tgactatgct gattactcct gcgacagtgg
     1741 cacttgccat ccctctttat aaaaacttcc gtttactgaa agagaatttt tatcctgtaa
     1801 tggctgcgat tttagcagga atagtcgcaa attgcttggt tagtgtaggg ttcggttact
     1861 tcttctctct tcataaagag atggtgatct cattactacc taaatctgta acgacggcta
     1921 tttctgtcga tttatctcat acgatgggcg gggtgaatgc cgtgacactt gctattgttg
     1981 tctctacagg tatttttggt tcgctgatcg ccactcatat tttccgccta ttcaagatcg
     2041 aaagtccagt tgctcgcggt gtggcgttag gaagtacttc tcatgctatt ggtacagcaa
     2101 aagcaatcga gattggtgaa atagaaggaa tcatttccgg acttgcgata tgcgtcaacg
     2161 gaatattgac cgttttattg ctaccgttat tctttcagcc ttttgctggc ttgttttgat
     2221 gcgaaaacca ctattttatg ctacaataac caaaacgttc gagcataaaa aaggagggaa
     2281 agacgtgtat ttgtcttccg agtatcttaa acaatttgat aaagatctat attataagat
     2341 tctcctccta gaaagttttg aggatcaagc ttggcatacc gctgcacaat tagcgcaagt
     2401 tgttcagctg gatgcccgca gcgtgtcgaa atacctgaac gagctgtcta aaaactatca
     2461 gcagtttagc ggtaagacac accctttgtt tacaaaaaat caccgttctg gttataactt
     2521 ttatgatacg ctcgattcaa ttgaacatga gcgttttttg atctatctcg tgcaaagtac
     2581 gctgaaattc cagttgctgc acgacatttt ttttgaagaa ttccatacaa tgtaccaatt
     2641 tgcgcaaaag cattacatta gtgaatctac tgctcatcga aaaatcaatg aatggaaaca
     2701 gcaactgcaa acttacggaa ttcggctgca acgaggtaca tatatcgctc aaggcgaaga
     2761 agaaatcatt cgactttact tgcatatgac attctggcag ttgtttcgag ggaaaatctg
     2821 gccatttgaa acgatttctc aaatggacgt aaagaatatg gcagaacata tcatggcttt
     2881 ttttaatgtt cgtctgaacg agatcaaaaa aagacgattg gaatatatgc taggtgcctt
     2941 ttttttaaga aaatcacaga agcattatgt tgtattgaat gagaaaaaaa gacggctgat
     3001 ttctgacaat ctgttgttcc agcgattttg ccaggtcatg gagccggtat ttcctaatta
     3061 ttttcaagta gaagacgagt taggcgcatt atttcttgta ctgatgactc gtgaagaata
     3121 ttatagtgat cctaagatac ggaagaaaat ttttgatttt catcaacaag caaaaacacc
     3181 gccctttaca gccctttcag aagcaaaagc cgctctttct ctttatcagg aagagcaggg
     3241 gctgccagca gaaaatctaa ccttcgaggc agaaaactat ttattcagca gtcatttttt
     3301 cgcctatctt tttcctaatg cgaaagaaac gattgatggc aatagtagtg attttattaa
     3361 tcatttagtt attgaaaaca aggaattgaa acaatggctc gtacattttt ttgaaagtag
     3421 acacaaacat cccaatcatc tggcatttaa aaatcatgcg tttttgatgg ggcggtatct
     3481 gactgtgttt aaaacattag gagcctttac tccacagctt ccaaagatca cgatcctttt
     3541 aatgacagat ttcccattat ttgaagagca actgttagag gagggattac ggacattttt
     3601 ccgtaatgag tatcagctga tttttctgcc aacagattat cgggggagag aagttgatct
     3661 tctgatttcg acaagtaaag tccaccgcaa accatgggcg gatcttgact attttattgt
     3721 gactgaagaa ctaaaattaa ttgattacat ccaactatct caaaaatttg aaatgattca
     3781 aaaacaaaaa ctattaaaac aataacaaag aaggagaaaa aagatgacat ttgaagaaat
     3841 tttaccagaa ttgaaaaaag gtgcaaagat tattcgtaaa ggatggagtg gatttgaact
     3901 ttatgttgtt ctcgtatccg atgatgaatt tgatggctgt ccggtgacgc cgtacttatt
     3961 gatcaaaaca agtgacgaag gtttttccag cttcgctcct acagtttgtg atattttagc
     4021 agatgattgg gcgatcgtag aatgaaaaac cagtatccag atctagctaa acaggttgta
     4081 tttatcacag gagctgcttc tggaattggt gctgcgcaag cccgagcgtt tcttgaacaa
     4141 gatgcttttg tatttggatt agatcaaaag ttcggtaaaa tggaagaaat ttctttgcga
     4201 tttcctgaaa cttttgccta cgcactaggt gatgttcgac aaatgaaaga actccaacaa
     4261 gcggtatcga aatgccaaaa tcattttggt gaagttacga ttttactgaa tacagcgggg
     4321 atattggatg cctacaaacc cttgatggaa actgacgaat cgctgtggga tctgatttat
     4381 gagacgaatg tgaaaagcat gtatcagctg acaaaaatca tccttcctga tatgatccgc
     4441 caaaaaagcg gaacgatcat caatatggct tcgattgctg gcttgattgc tggtggaggt
     4501 gggatcgctt atacaagcgc gaaacatgcg attgtcggat ttaccaaaca actcgctctg
     4561 gattacgcag gacagggtat ccgagtaaaa ggaattgctc caggagccat ccaaacacca
     4621 atgaatgcgg ctgattttga agggaaaggt gagatggcag agtgggtggc aaatgaaaca
     4681 ccagtcaagc gatgggcaca gccggaagaa gtagctgagc tgacgttatt tcttgcctct
     4741 cctcaagcaa gctacataca aggaacgatc atcccaatcg acggcggatg gttgttaaaa
     4801 taaccttgtg tttttttcaa gcttctgtat gataaaacta aagagagaaa caacggttga
     4861 tgataaaaaa agtcgatgac tgttcccgac attcgatgcc gaacagccag ttcaaaaaat
     4921 tctttttaat aatcgttaat attcgaaact aagcggcttt tcacgacctc ttgatataaa
     4981 cgatattcaa aagcttgcaa ctgcggtaac aagtccagcc aaatgaaaaa tataaggagc
     5041 tatttttatt tgaccattct tattacttgt tgcagggcag ctttttcact accttctgcg
     5101 acacttcagt tggaattttc ttcccatcct ttggtcgcgg tcattaccaa gataaaatta
     5161 tcttgcaaag gagaaataga acatggaaaa aaataatacc cgttcagcca aacgctggac
     5221 aacgacagat gtgactcgga tggcgctagt cactggttta tatgtggcag tgacgctggt
     5281 tttgtctgtc tttagctttg gagtgattca gattcgtctc tcggagatgt tcaactatct
     5341 gtccttgtat aacaaaagat acatcgtcgc ggtaacagcg ggagtagctt tggctaatat
     5401 tgcttctcct cttggactga tagatgttgc agtaggaagc gtcagcacgc tgcttgtatt
     5461 gcttatcaac tataaaatca ctagtcgcat caaaaacatg aaaatcaaaa tggccgtgac
     5521 agcatgcgta tttgcttttt ccatgtttac agtagctggt cagctaacga ttttatatca
     5581 actacctttt ttcttgaatt ggtgggtgat tgctttagga gaattggcat caatgattgt
     5641 cggtgggata attatttatt ggatcggtaa aaaagtggat ttgtcaaaat aaagatctgt
     5701 ttaatttaaa atagggcagc atggaagaga ctgaatgttg agtcttcttc catgctgctt
     5761 ttttgattaa tttgtatgaa gaaatcgatt tagccgtttt ttcatcaaga taccgatata
     5821 agctgctcca gttgccttga ttgcttccgg cagtaaaaac ggcagtagtc cactggcaaa
     5881 tgcagttgta aaagtaagat caccacttat tttcagccag atcgttccgc agatcaatgc
     5941 agcaagagag cccattagat tggcaaagat tgcatataag taatgagcat ccgttctttc
     6001 gatcatccaa ccagttatcc acgtttgtaa gagaaatcca atcaaatatc cgccagttgg
     6061 cccaaataaa acagctatac ctccagtcat ccctgaaaat acaggaagac caatcaagcc
     6121 aagtaaaaga taaactccaa cagatatcaa tcctagtttt cttccgagaa aagttgctgt
     6181 taatccaata gcaaatgttt gccctgtcaa aggaaccaaa ccaaaaggaa tggtcatctg
     6241 agaaaggaca gaaataatcg cggcaaattc cgcagcaagt atcaattctt tcaatgttcg
     6301 gttcataacg ccctcttttc gttaacttaa tatttttatt tggttaacga aattgtaaaa
     6361 caaagtgatt actttgtaaa tagaaaatat aaaaaaagac cagctatcca atagatagcg
     6421 gtctttttca agaaagcttt tgaaattata agctttggtt gtagtattca acgacaagag
     6481 cttcgtcgat ttctggatat agttcgtcac gttctggtaa acgagtcaat gaaccttcca
     6541 atttttcagc gtcaaagctt acgaatgctg ggcgtccaac agttgcttct acagcttctt
     6601 ttactgttac gatgttttgt gatttttcac gaactgagat cacttgaccc acttctacgt
     6661 ggtatgaagg gatatcaacg cgtttgccat caactaaaat gtgaccatgg ttaactaatt
     6721 gacgtgcttg acgacgagtc gttgcaagtc ctaaacggta aacaacattg tctaaacgtt
     6781 gttctagtaa gatcatgaag ttaacaccat gtttaccttc tttgattttg ctagctttta
     6841 cgaacaagtt acggaattga cgttcgttca ttccgtacat gtgacgaagt ttttgttttt
     6901 cagttaattg catgccgtat tctgattgtt tgccacggct attaggtcca tgttgacctg
     6961 gtgcgtaagg gcgacgtgct aattcttttc ctgtgcctga caatgagatg cctaagcgac
     7021 gagaaatttt ccatgatggt cctgtataac gagacattaa aatttcctcc aatgaatata
     7081 agtttttgga gtaaaataat ccgttgaaaa attcataatc atatagtttg ttcttcaatc
     7141 ttcacctttg cagccgcggt tacacaatcg aacccgagac ggggcaacaa actagggatg
     7201 agttattatt tttctgctgc attattttac acaaattaca gtatatgata gatcatccac
     7261 gaatgtcaag ataattagta cataaaaacg ctttacaaac ctaggctgtc tttataaaat
     7321 gagagggtat gtgcaaatgg aaaaaagcga aggaagttta aggagaaaat caatgaatcc
     7381 ggtatcacca ttatttgaga aaatcgatgc ttcaattgaa aagaaggcga atctttttga
     7441 aaacagcttt tcacgttacg ctgttcgagc ggttctcgcc tgtttgtttt tgactttagg
     7501 aacggctgtg gcatttgcga tcgcgatgaa gggggaagat atcgcgcatg gtctaggaaa
     7561 gattttatac gcatttatgt tcagctgggg tctggtcatg attctataca tgaacgccga
     7621 attaggaaca tcgaatatgc tttatatgac cgtgggtgtt tttcggcaga gacttgcttt
     7681 aagtaaggca gccaagattc tatttgcctg tatttttttc aatatgattg gcggtatatt
     7741 atgtggattt ctaatttcta tgactacgcc atttcaagat ttaacgttgg acaactttat
     7801 gttagaatca atagcagtaa aactagcaaa accttcagca acgattttgg tggaagggat
     7861 gtttgcgaat atcgttgtca ataccgctgt actgatcagc atgcggatga aagaggatgt
     7921 cggaaaagtg atcacagtcg tattcatcat tttcattttt gcttttttag gatatgaaca
     7981 cgtgatagcg aattttcctg cctttacgct tacttacttt gcttctcacg gacagatgga
     8041 tggaatgaca gtcagcaatg tgttacataa tttgtttttt gctttggcag gaaattatat
     8101 tggtggcggt ttagtaatgg gactgggata tgcttggctg aatcagtcaa aatcatccta
     8161 tgtggactaa aaagtcgttc attaaagggg aagtgccatg gaagttttag tttttccaaa
     8221 aaatgaggaa aaagcgttac aaatggaaaa gtacatgaaa aatctttttt cctttgctgg
     8281 agtacctaag ccggaacgcg ctcttttaca acaaccgtta ctggatgaga gcaaaaattt
     8341 atctatttct gaagttttag cttttgttca ttattattat gaacaaatcg aaagagagta
     8401 tcagtatgta gcgatcgatt tagtccagaa aaacatcaaa cggcttacat ttgaagactt
     8461 agtcgtattg ttgccactag ttcagaaaaa agaatggtgg gacagcatcg actcttggag
     8521 aaaagtatac agcacatggt gtaaagccta cccagagcag cgtgaagaag tctttcaatt
     8581 gtttgaaaaa aaggaagatt tttggctacg tcgaatagca atcacgttgc aattaggtta
     8641 taaacagcaa acagacacag gactattgat ccgagcaata gacgaagatc gtcaaacaaa
     8701 ggaattcttt atccaaaaag caattggttg ggcattacgc gattatagta agaccgatgc
     8761 cgattgggta aaaagacagc tgacaacaga tttgagtaag ttagctgttc gagaggcaag
     8821 taagtattta taagaaagcg agacgacaag agatgagacg caaattattt gcttttgata
     8881 ttgatggaac gttgttagat tcaaacaaac aagctttaga tagcacaaga gaagcattgg
     8941 aaaaattacg caaagccggt catctggtca cgattgctac agggcggagc cgatttcatg
     9001 cgcaagagat cattcgcgat cttgatttta cgaactacat tttatgtaat ggagcggccg
     9061 gttttttaga ccatgaacaa gtatataaga atttactaga tcgcgaccag ttggatgcgt
     9121 tcgttcaaga agcgcatcat gagcagatcg atacagcttt tgtcagctta gacaatatca
     9181 agcgttcttc ttccaatgat gtaacggtga tggaagaagc gatgcattct ttcggtgctg
     9241 tcttgcctga attagatcag ttttttgcag aggaacaaga aatataccaa gcgttagcat
     9301 ttttcgatca aacatatgat gggaagttct cttcatatga taaaattcga tttgtccgct
     9361 ggcatgagaa cagtgtagat gtcgtaccta aaaatggttc taaagcagcg acaattctgc
     9421 acttagcaga acaagtaggc atccctcatg aagacatcat cagctttggc gatggacaaa
     9481 acgaccgtga aatgttgcgc atgtcaggaa ttgggatagc tatggggaat gctgtgccag
     9541 aagtacaagc agaagctaag atggtcactg atacaaacga tcaggatgga atttggaagg
     9601 cattgaaaga gttaagcgtg atttaaaaga ggttgtgaaa aagctgttct gcatcaagta
     9661 ataagcccag ccaaacaaaa atagctcctc ctatttttcg tttttctggg cttattaccg
     9721 cagatgcaag ctttcgaaca ccgtttaagg aagaggtcgt gacagaagtg gtcagtttca
     9781 agaaataaga agggatttac gaaaattgct tctcaaattt ttgtaaaatt acggcttatt
     9841 tctgaagaag tagatgaaac aacgtttatt cgggaaatgg gactgtgaca agatgtttgt
     9901 catagtccca tttttttatt ttatttttca aaactaagaa ataccaggaa agagttcctt
     9961 ttgtttgacc ttttctttct tattgaaaac aactataata tagagtgatg ttaaaagaga
    10021 agaggaattt agtgtgcggg tattgtttat tggtgatgta gtaggttcaa tgggacgaga
    10081 gatgattact gaatatcttc ctagattgaa gaagaagtac cgtccgcaag taacgattgt
    10141 aaatggtgag aatgccgctt ctggacgtgg aatcacagaa aaaatctata aaaaattttt
    10201 gcaagacggc gtagatgtcg tgacgatggg gaatcatacg tgggacaacc gcggaatctt
    10261 tgagtttgta aatgaagcta aaaagatggt tcgtccggca aattttccgg aagggacgcc
    10321 tggacaaggg atggtctttg tcaaagtgaa ccaagtcgaa ctggcagtga tcaacatgca
    10381 ggctcgttct tttatggtgg acttggatga tccttttcgt aaaatgaatg agctggtaga
    10441 agaagccaga aaacgtacgc cgatcatttt tgtagatttt catggagaaa caacaagtga
    10501 aaaacaagca atgggctggt ttttagatgg gaaagtttca gcagtggtcg gtacacatac
    10561 acacgttcag acaaatgatg cgcgaatttt gccaagagga acagcgtatc tgacagacgt
    10621 tgggatgaca ggtccctatg atggtatttt gggtatgcgc agagaacctg taatcgaaaa
    10681 gtttctgact gcattaccaa aacgttttga agttgtggaa caaggccgat cgattttaag
    10741 cggctgtatc ttggaattgg atgatacgac aggtcatgca aaagaaatcc agctgattca
    10801 gatcaacgat gatcgaccgt tcatggagta gaaagagagg cagttttttg cagcaggagc
    10861 cggaaattca aaaagaagta aggaaactga caaaactttt aagagagaat gaaacgatca
    10921 ttcgctacaa agaacttgaa gaaaagatcc agcaaaacca gtatttagct gagttgagag
    10981 aaaaaatcaa acaagcgcaa aaagatgctg ttcattttgc ccactatgac aaaccagcag
    11041 cagaaaaaga agcaatcaaa caagcggatc aattcatgca agaatttgat cagcatccgc
    11101 ttgttgtagc ttatagaaaa cagctgttgg aagcagatga tctgcttcat catttaacaa
    11161 ctatgataca agaagaaatc aatggacaaa tagaggagga aaaacatgcc tcaaaaaact
    11221 aaaaatactc ctatgatgga acaatatcta agcatcaagg cccaatacca agatgctttt
    11281 ttgttctatc gtttaggaga cttttatgaa ctattttacg aagatgcgat caaagcgtca
    11341 cagcttttgg aattaacgtt gacgagcaga aatcgaaatg cagaagaacc tattcctatg
    11401 tgcggtgtac ctcaccatgc agcacaagga tatatcgatg ccttgattga aaaaggctat
    11461 aaagtagcga tttgcgagca ggtagaagat ccaaagacga ctaaagggat ggtcaagcgc
    11521 gaagtgatcc agctagtcac tcctggtaca gtcatggaca gcaaaggatt gtcggcaaaa
    11581 gacaacaatt ttttaacagc tgtagttagt caaggaaatg aatttggctt tgcttatgcc
    11641 gatttaagta cgggagaatt gaagacggct cggttgcatg atgaagaagc tgttttaaat
    11701 gaagcctctg cattgcagac aaaagaactc gtattaggca gtgagatcac tgataccctt
    11761 cgtgaacagc taagcgggcg tttagggcta gtcttttctc aacaaaatga gatggaagaa
    11821 aacgcggaat ttagcttttt gacaagcgaa ctagtcgatc cgctggaaaa agaagcaacc
    11881 ggcaaactat tgacttattt atcggtgaca caaaagcgga gtttagctca tatccaaaaa
    11941 gcagtagaat accaaccaga tcattttttg aaaatggatt attattcaaa attcaaccta
    12001 gaattaagcc aatctattcg gactgggaaa aagcacggca ctcttctatg gttgttagat
    12061 gaaacaaaaa cagcaatggg tggccgttta ctaaaacaat ggctagaccg tccgcttatc
    12121 caggcaaaac aaattaaagc aagacaagaa atggtcgctt ctttgctaga cagctatttt
    12181 gaacgtatcg atttgcagtc agcattaacg aaagtctatg atctggaacg cctagcaggt
    12241 cgagtagctt tcggtaatgt caatgggcga gacttgatcc agctgaaaac ttctttagaa
    12301 caagtgccaa tgatcagagg gttgattgaa ggaatcgatc aaggtcaatg gcaaagtctg
    12361 ctttcagaaa tgcagccgat ggaccatctc gtccaattga tcgaccaagc tatccaagat
    12421 gatccgccgt tacaaataac ggaaggaaac atcatcaaag atggattcaa tgagcagctc
    12481 gatatgtacc gttctgctat gagaaacgga aagaaatggt tggcagaact tgaagcaaaa
    12541 gagcgtcaag aaacaggaat caagaactta aaaatcggat ttaaccgcgt tttcggttat
    12601 tatatagaaa ttacaaaagc aaatcttgga aatgcagaac tggagaagta tgaacgaaaa
    12661 caaactttag caaatgcaga gcgtttcatc acaccagaac taaaagaatt ggagacacaa
    12721 atcctcgaag cagaagaaaa atctgttgat ttagaatatc agttgttttt agctgtgaga
    12781 gaagaagtca aaaaggctat ccagccgcta caagtacttg caaaagcaat cagtgcagta
    12841 gatgtgctac aaagttttgc aacgatcagc gaacgctatc agtatgttcg gccagaactt
    12901 gtcagtgaca agcatcagct tctgatcaaa gacggacgac atcctgtagt agaaaaagta
    12961 ttaggacatc aggaatatat ccctaacagc gtagaaatgg cagaagacga aatgatcttg
    13021 ctgattacag gaccgaatat gtctggtaaa agtacgtata tgcgacagtt ggcattaact
    13081 gttctgatgg cgcaaatggg ctgtttcgtt ccggcaaaac aagcagtttt acctattttt
    13141 gatcggattt ttacccgaat tggtgcaagt gacgacttaa ttgctggaca aagtactttt
    13201 atggtagaga tgatggaagc caatcaagcc ctgcgttatg ctacgccaaa cagtttgatt
    13261 ttgtttgacg aactagggcg aggaacagca acctatgacg gaatggcgtt ggcccaagca
    13321 atcatcgaat atatccatcg tcatgtacaa gcaaaaacac tcttttctac tcattatcat
    13381 gaactgacgg tacttgagaa agaacttccg cagcttaaaa atgtccatgt tggagccgtt
    13441 gaaaaagacg gagaagttgt cttcttgcat aaaatgatgg atggacctgc ggataaaagc
    13501 tacggcatac atgtggcaaa gatcgcagga ttgccttcca atctactaga acgagcagca
    13561 gatatcctgc atacgctaga aaatggcgat aacggacacc atactgttcc agctgaagta
    13621 gaagagaaaa ctaaaacagc caaacaggag caaataaaac cacctgcaga aaaagtaaat
    13681 caagaccata tacaagaccg tgtaaaagaa gaaaaccaac agctttcttt attcagtgaa
    13741 ttatccgaaa atgaaacaga agtcattgaa tcactgaagc aaataaattt actagaaatg
    13801 acgccaatgg atgcactaaa cacattatat gaattacaaa aacaactgta ggaagaaggc
    13861 ggatatcgat ggccaaaatc caagaattat ctgaacgttt agctaatcag atcgctgcgg
    13921 gagaagtagt ggagcgacca gcttccgttg tgaaagaatt agtggaaaat gcaattgacg
    13981 ccggcagtac tcagattgat atcttagttg aagaagcggg actgcggacg atccaagtga
    14041 ttgataacgg agaaggcatt ttggcagacg acgtagagaa tgccttcaag cggcatgcga
    14101 caagtaagat ccataatcgc gacgatcttt ttcgcatacg aacgctgggc tttcgagggg
    14161 aagctttgcc aagtattgcc tctgtttctg aaatgactct ggaaacggcg aatcaggaag
    14221 aaaaacaagg cacctatctg tcgctgaaag gcggagaagt tattgagcat cgaccagcac
    14281 cactgcgtca aggaacgaag attaccgtca gcaatctgtt tttcaatacg ccagcccgct
    14341 tgaaatacgt gaaaacactg cacacagaat tagcgaacat cggggacatc gtcaatcgac
    14401 tggcattgag tcatccatcg attgcttttc gtttagtaca tgatggaaac aaaatgctgg
    14461 caactgctgg aaatggcgat ctgaaacaaa caattgcggg aatctacgga ttatctaccg
    14521 caaaaaaaat gctgaaaatc gaaggaaaag atctagattt tgagttattt ggctatgttt
    14581 cattgcctga ggtgactcgt gccagtagga attacttatc gacgatcatc aatggacgtt
    14641 ttatcaagaa cttttccttg aataaagcaa tcgttgcagg ttatgggtca aaattaatgg
    14701 tcggtcgttt ccctattgct gtattggaaa tcaaaatgga cccgctttta gtagatgtaa
    14761 atgttcatcc gacgaagcag gaagtccggc tgtcgaagga aaaagaacta acagagctaa
    14821 ttagtaacgc cattcaaaaa gcattgtctc aagaaaatct gattccaagt gcagcggata
    14881 atctacggtt taagaaaaaa gtacctgatc agcctaaaac agaacaaatc gaaatcgatt
    14941 tgtcctacgc ttttgaagaa gaaaaaccta aaaagccaag tagtttgaat tttgatcgag
    15001 agacaggtac ctttttcgta gaagaactaa agaacacatt tgttgacaga attgttgata
    15061 agtctgtgga aaatgttgaa aactattctg gtttttctga agaaaacacc acttatccag
    15121 aaaaaacagc gaaaacggtg gataagtttg gtgaaaatgt tgataacttt gtagaaaaaa
    15181 cagtggaaaa atctgaaaag aaagaatttc ccattgtgga taacatggag aaaacaaatg
    15241 aagaacaagc agataaatcc gtagaagagg atgaaaagat ttacgaggaa gaattgacac
    15301 tccatcctga atttgatttc actcaaggaa acgacaaaac attagaacaa gccatcaaga
    15361 aattagaaac ggaaaaacca gtacaacgtt ttcctcagtt ggaatatttt ggacagatgc
    15421 acggaacgta tctttttgcc caaagtagcg atgggttgta catcattgat cagcatgctg
    15481 cccaagagcg cattaagtat gaatatttcc gggaaaaaat tggggaagta tctgatgatc
    15541 tgcaagaatt gcttgttcca tttgtgttag actatccaaa cagtgatgct ttgaaattaa
    15601 aagagcaaaa acacttgcta gaagatgcag ggatttattt agaagaattt ggtcaaaaca
    15661 gctttattgt acgggcccat cctacttggt atcccaatgg agaagaagaa tcgattattc
    15721 gggagatgat cgatatgctg ctgacaactg gaagtgtgag tgtaaaaaaa ttccgtgaag
    15781 caacagcaat catgatgagt tgcaaacgat cgattaaagc caaccattat ttgaatgaag
    15841 cacaagcacg tgtgctgctg aaggatctgg cacgatgcga aaatcctttc aattgtcccc
    15901 atggccgtcc agtattgatt cactttacca attcggatat ggaaaggatg ttcaaacgaa
    15961 tccaagaccc gcattaagtc aaagagaaga gggaaaacat gaagattgta ttagcatccc
    16021 aatcaccaag aagaaaagag ttattggctc atatcgtttc agaattcgag attgaacctg
    16081 ctgatatcga tgaaacgcca tttccagacg aacagcccgt cgattatgtt cgtagaatgg
    16141 ctgaagaaaa agcacgaact gtctgggaac aagatgaaaa agaagaccat ttagtgattg
    16201 ccagtgacac gactgttgtt ctcaatcaag agatcatggg taaaccagaa gatctggctg
    16261 aggctgaatt catgctgaaa aaactaagcg gggaaacaca tactgtatat acagctgttg
    16321 ttttaaaaac aaaggaacga gaagaacgga tactagcaga agcacatgtc actttttatc
    16381 ctttgacaga cgaagagatc aaacgctact tggagacggg ggattatgcg gataaggcag
    16441 gggcatacgg catccaaaat gctgcgagtg tattcgtcaa agaaatttct ggagattatt
    16501 acagtatcgt aggatttccg attggtgctg tcaatcaggc actgaaaaca ttcatttaaa
    16561 caaaaaagcg gactatctga ttttcgattg aaaatccatg atagcccgtt ttttttattg
    16621 aaaagattac ttatctactc tgactaagat tttacttcag ctttaccgac tgcttcgccc
    16681 gggcgaacaa gtcctgattg gtcaagagaa agtcctgcaa ctttttcatt attggtcaaa
    16741 gcaacaataa tgtcagtttt cttgccagct tgttctattt ctggcaaatc catttcagca
    16801 atcagggtct ctggtgtgac aaggtatcct tctttcacga atacattgaa aggaacaccc
    16861 tttagttcaa cagtatctaa tcccatgtga acaagtactt ccagcccctc attagaaaga
    16921 ataccaatgg catgttttgt ttcaaagaca ctcgtcactt taccgtttac tggtgcatag
    16981 actttgccgt ttgacggttc gacagcataa ccgtcaccca tcattttttg tgagaaaact
    17041 gggtcattta cttcagttat ttccttgatg gtccctgcag cgactgcgaa taatgtttct
    17101 tttgttgcaa ctgccgtacc agttgctgaa acaggttcga aagactgtgg cgttgctgtt
    17161 gcgccatttg ctgtttgaag ttgtacatca gcagcatttt caggaacagc aggatcaatc
    17221 ttattcaaaa ttccctgacg tctaaagatg atcgtcaagc caaatggaat gatgacagca
    17281 atgatcattg cgatgatgaa aggtacccat gtttctgctt ggatagctag tattcctggc
    17341 aaaccgccta ctccgatcgc gttagctcga acacccatca agttagcaaa catgccagct
    17401 aatccagagc cgaccattgc agcgacgaaa ggatagacgt atttcaaatt gattccgaac
    17461 atagcaggtt cagttacgcc taagtaacaa gaaatcattg ctggaatcga gatttgttct
    17521 tcttttttat tcccacgatg taagaagatg atggctaaga ctgcagagcc ttgggcgata
    17581 tttgataaag caatcatcgg ccataaattc gttgatccga aatctgcaat cagctgcata
    17641 tcgatggcat tggtcatatg gtggagacca gtaatcacta aaggagcata caggaatccg
    17701 aatacggcac taaacaacca gctgatagaa gacgtcaatc cagtatttac gatatttgag
    17761 atccagcttc cgactgtcca gccgatagga cctaaaataa cgtgtgcagc taaaacagtt
    17821 ggtaacaatg aaaacaatgg aacaaaaatc atcgagatcg actgagggat atatttgcgg
    17881 aagaagattt ctagatacgc taacataaac cctgccagca tggctggaat aacttgcgct
    17941 tgataaccaa tcatctggac ttgagcaaaa ccaaaatccc aaaatggaat atctgctgcg
    18001 gctgtggaag ctacactata agcatttaat aattgcggag aaactaacgt aattcctaaa
    18061 acgattccta atatttgtgt agtacccatc ttttttgcaa tactccaagt gatacctact
    18121 ggaagaaaat ggaaaattgc ttcacccggt aaccacaaaa aggcattgac accattccaa
    18181 aacttcgagt gttcaacgat tgttccacca agactttcaa actggatacc ttcaagtaca
    18241 tttctgaatc caaggatcaa tccaccgaca atgattgcag gaatcagtgg tgtaaagatt
    18301 tctgctaaaa cggcaatagc acgttgaacg ggatgaaggt tttgttttgc agctgctttt
    18361 ccttgttctt tggaaacccc ttcaactcca gatactgcaa cgaagtcgtt ataaaaagta
    18421 gaaacatcgt ttcctatgat gacttggaac tgtcctgcat tcgtgaacat cccttttacg
    18481 ctaggtatat cttcgatcgc tttttcgtcc gcttttcctg gatcattcag gacaaaacgc
    18541 atacgagtcg cacaatgact aactgcagca atattttctt ttccgccgat atcatgtaac
    18601 agcttctcgg catctgcttg atattttgcc atatcaataa acccctttct cttaacttgt
    18661 ctatgcaagt tgtttcttca tttactatac tttttttcgg agagaattgc aagcgtttta
    18721 ttaaaaagta cgagagaaaa tgatatcgtt ttaaaaagct taaaataagc ttttcttaaa
    18781 aataggaaca ttgaaataca aaaagagctg tagtatagtg aagaaatgat ggaagaacga
    18841 gtgagggaaa aaacatgaat aagttcaatg aaattttttt agatttggaa cagaaaattt
    18901 tagcgaaaga atatcctccg cattcgcttt tacccagtga gaaccaattg atcaaaattt
    18961 atggtgtttc acgagaaaca attagaaaag cgctgaatct tttgactacg gaaggttata
    19021 ttcaaaaaaa acaaggaaaa ggttcgatcg tactggatcg gaatcgcttc gactttccta
    19081 tttctggatt gactagctat aaagagctac aggagaccca acgtattcca agtgaaacga
    19141 tcatccatac gttagaagaa acagaagtga caaaagcatt gaacgagatc actggctggg
    19201 agatcggtgc gcctgtttgg catttgatac gcgaaagaaa aatcaatggc gaagtagtta
    19261 tattagacac cgattatcta ttaaaggaaa tcgttcctat tttaactcca gcacaagcac
    19321 aaggatcgat ctatgaatat tttgaaaatg agctttcttt gacgattgct tacgcgcaaa
    19381 aggagatcac agtagaagaa gtaacggaaa tcatgaaaac agcgatggat cttcacgatg
    19441 acacttatgc agtagtagtc agaagcctcg ttcatctgga agatacccgc tgtttcgagt
    19501 attctgagtc ggttcaccga ttagataaat tccgtttcgt tgagtttgcc cgacgccgaa
    19561 aagtgtaaat agttcaaaag aaagcaaatt gttggcatct gtccactttg gcgctatgat
    19621 aggatcacaa agggggcatt tttaatggat aaaccaataa gaccagaaaa agaagaactg
    19681 aaagaaaagt tatcacccat ggcttatgca gtcacacaag aaaatgcaac agaacgacca
    19741 tttagcggga aatatgatga tttttacgaa aaggggatct atgtagatat cgtcagcggt
    19801 gaaccgttat tcagctctgc agaaaaatat gatgcaggtt gtggctggcc atcattcagc
    19861 aaaccaataa cgaaaagaca agtgcgcgaa aaagccgatt tttctcatgg catgcatcga
    19921 atagaagttc gaagcaaaga agcagattct catctcggac atgtgtttac cgatggacca
    19981 gtagatcaag gtggtctacg ttactgcatc aattctgcct cactgaaatt cattccatat
    20041 gaaaagatgg atgaattagg ttatggggaa ttcaaatcac ttgtagaata atggaaatac
    20101 acaaagagcg gtacaatacg gggatttact cgatcgtccg ctcttttttt attcaagcga
    20161 ttgctatttg aaagaaacgc tccctagact ccttttttca caaggtgaaa tgtgctacaa
    20221 taaagggaac gtatgtgtgc aaaagaaaga ggactagaat atgtatgaat atctgacagg
    20281 tgtggttacc tttatcaacc cctattacat agtagtagaa accaatggta tcggctacca
    20341 gatcgcacta ggaaatcctt accgctacag tagcaaacta aatcaaaaaa taaaaattta
    20401 tgtccatcag gtaatacgtg aagatgcgca tcttctgtat ggatttgatt cgctagaaga
    20461 aaagcaactg tttttgcgat tagtaagtgt ttcaggaatc ggtccgaaaa gtgcccttgc
    20521 aatcatggca agcgaagacc actccggttt gattcaagca gtagaatcca gtgatgtcac
    20581 ttatttgacg aaatttccag gagtagggaa aaagacagca caacaaatga tcttggatct
    20641 aaaaggaaaa ttcggtgagc tgagtatcga tacacctttt agtctatttg atgaagcaaa
    20701 cacaaaagac gctacagctc tatctgaagc aatagaagct ctctcggctc ttggttacag
    20761 tgatcgagaa atcaaacgtg tagaaaaaca attaaaagaa gtagataatc agacaacgga
    20821 tgagtatcta cgccaagcac tcaagctaat gatgaaaaaa taaggaggag accaaatgac
    20881 agatgaatac ttgttatcac cagaatctgg cgaaaatgaa ctgagtttgg aaaaatctct
    20941 tcgaccacag ctgctttctc aatatatcgg acagagaaaa gtcaaacaag aacttcagat
    21001 ttacatcgaa gctgccagaa atcgggaaga acctttagat catgttctgt tgtacgggcc
    21061 tcctggatta ggaaaaacga cgatggccat ggtcattgct aatgagatga ccgtaaatat
    21121 tcgtacgacg agtggccctg ctatcgaacg tccgggtgac ttagtagcaa tcttgaatga
    21181 actggaagca ggggatgtcc tgtttatcga tgagatccat cgattaccta gagtggtaga
    21241 agagatgctt tattcagcta tggaagattt ctatgtagat atcatggtag gacaagggcc
    21301 aaccgcacat ccggttcatt tccctcttcc tccctttact ttgattggcg cgacgacacg
    21361 ggcaggtatg ctttctgcac ctttacgaga tcgatttggc atcatttctc atatggaata
    21421 ttatgacgaa caggatctgc aggagatcgt tttacgttct tcggatattt ttcaaacaga
    21481 gatcattgaa gaaggggcat tggaaattgc tcgccgatca cgaggaacgc ctagaatcgc
    21541 taatcgcttg ctcaaacggg ttcgtgattt tgcgcaagtc caaggagacg ggaaaatcga
    21601 tcgtcctata gcggatcagg cacttacttt gctacaggtc gatcaggctg gattggatta
    21661 tgttgaccag aaattgttgc gaacaatgat cgagttatat ggcggcggac cagtagggtt
    21721 aagtacgctt tcagtaaaca taggagaaga gcgtgagacg gtagaagata tgtatgaacc
    21781 gtatctgatc caaaaaggat ttttaaagcg tacacctaga gggcgaattg ctactgctta
    21841 tgcctatgaa cattttggtt acgactatca tatatgattt ttgcttttag attggaggaa
    21901 aatgaatgag tgatacaaca cgtacgaaac aattgatcaa aaacgtattg attgatttgt
    21961 gccaaacgca atcttttcga aaaatcagcg tccaaaaaat cgcaaacgaa gcaaaaatca
    22021 atcgacaaac attttattat cattttacag ataaatacga tttgcttcgt tgggtctatt
    22081 gtgaggatgc attgcattat ttagatccac aatgtctttc acttgataat tgggaagagc
    22141 aagcattgaa aatgctgaaa gcaattgctg agaagaaaga attttatgca aatactgtgc
    22201 tttctgaccc agaaatttta caaaaagagt tttctcaact gatccaaaaa tcatttatca
    22261 agatttttga acaaatggat gaagaaagac aactttctgc aagagacaaa ctattttatg
    22321 ctcgtttttt ttcttacggt tgcagtggtg tattagtcaa ttggatcacg aatgaatatg
    22381 aagaaacacc gatggaaatc gctatgcagc ttttccgttt agccaaggat acggaattat
    22441 attcttatcg tctttatgca aaagaagagt tggaagaaca gtaatagaaa aattttttgt
    22501 tggaaaagca ataatcgcta aaaggagatg gggaaatgac aaaaatttta tttgtttgtt
    22561 tgggaaatat ttgccgttca ccgatggcag aaggcttgct gagaaaacga atagcattag
    22621 aaggaaggga agcagaattt tttgtggatt ctgctgctac aagcacgtat gaagttggaa
    22681 agacaccgca ccctggaacg aaaaaaatat taaaccaaga aaaagtagat atgacgaata
    22741 tggttgctcg gcaaatcaca ccccacgatt ttgaaacttt tgattggatc attggcatgg
    22801 atcaagaaaa tgtagaagaa ctgaaacgaa gagcaccacg ctcagcgcag gggaaaatcc
    22861 atttgttttt aagtagcatt cctggaaaag aaaaagaaaa tgttcccgac ccatactata
    22921 ccaataactt tgatgagaca tatcacttga tcacagaagg cctgaatcat tggtggaata
    22981 tttggattga tgaaaataaa ataacaaata atttgtaaag ttaacgcttt ctttttaagg
    23041 cgatgttaga gcatttcctt gtttttttgt aaaatgttag gtaaaataaa attaattaag
    23101 taacactttc ttagctaaag gaggagtgca ttcgatgaac gaagagacag tatcgccaat
    23161 gaagaagctt acgaagttag ttactcatac ttcgtatcaa ctttttgatt cttttgtaaa
    23221 tgacactgaa gaacatgcta agaatgtacg attgcaagga aagtttattc ttgagctgca
    23281 aaaagctgct actgaacata gtctagttat cttacaagta agagcaaatc gtttgagtag
    23341 caagtatgaa acgatctcag gctgggtggc gacaaaaacg gtcgaaaaag accgagtgat
    23401 gatccgccta caaaacgaag agcagcagat ccggatggtt cctgtagaac aaattgaaaa
    23461 aataacaatc ctttcaccag caggggaaca ggaacggtta tcaaaataat ttataccaga
    23521 tagaaaagca cgtatgagct tgtaccatca atcattacta atcaaaaata accgaatgct
    23581 ttaagctgat atcagtaaaa cggaacgttt taggatgagt atcatagcat gacagcattc
    23641 ggttgattag ttgaatcgat tggatggtac aagttttttt gataaaaaaa ggtcctgcta
    23701 gccggggagc tagcaggata aggagttaaa aatgaaaaag tgttgttagg gttgtttgtt
    23761 ggtatgctta tataatagtc attagttgtg aatattttgt gacgaaagtt ttcgaaagtt
    23821 gttaagtttt
//
LOCUS       DACDKM010000030        23313 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20834_2016
            SAMN12640370-rid9891663.denovo.030, whole genome shotgun sequence.
ACCESSION   DACDKM010000030 DACDKM010000000
VERSION     DACDKM010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640370
            Sequence Read Archive: SRR10040891
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23313)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23313)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742835.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742835.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:04:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,012
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 29 of 75
            Pseudo Genes (incomplete)         :: 33 of 75
            Pseudo Genes (internal stop)      :: 19 of 75
            Pseudo Genes (multiple problems)  :: 5 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23313
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640370-rid9891663.denovo.030"
                     /isolate="E20834_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            2..74
                     /locus_tag="IV334_001171"
     tRNA            2..74
                     /locus_tag="IV334_001171"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:35..37,aa:Asp,seq:gtc)
     gene            78..150
                     /locus_tag="IV334_001172"
     tRNA            78..150
                     /locus_tag="IV334_001172"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:111..113,aa:Phe,seq:gaa)
     gene            155..225
                     /locus_tag="IV334_001173"
     tRNA            155..225
                     /locus_tag="IV334_001173"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:187..189,aa:Gly,seq:tcc)
     gene            238..311
                     /locus_tag="IV334_001174"
     tRNA            238..311
                     /locus_tag="IV334_001174"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:272..274,aa:Ile,seq:gat)
     gene            323..394
                     /locus_tag="IV334_001175"
     tRNA            323..394
                     /locus_tag="IV334_001175"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:356..358,aa:Glu,seq:ttc)
     gene            660..1853
                     /locus_tag="IV334_001176"
     CDS             660..1853
                     /locus_tag="IV334_001176"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HAP8943258.1"
                     /translation="MVERHLFTSESVSEGHPDKIADQISDAILDAILKQDPTARVACE
                     TSVTTGLVLVFGEISTTAYVDIQKVVRETIKEIGYTRAKFGFDGDTAAVLVAIDEQSP
                     DIAQGVDEALEIRDEDKKDVLDEIGAGDQGLMFGFAVDETPELMPLPIALSHRLVRRL
                     ADLRKSNELTYLRPDAKSQVTVEYDDQGQPERVDTIVISTQHDDAVDNETIRHDVIEK
                     VVKEVIPAELLDDQTKYYINPTGRFVIGGPQGDAGLTGRKIIVDTYGGYARHGGGAFS
                     GKDATKVDRSASYAARYIAKNIVAAGLARKAEVQLAYAIGVAQPVSISINTFGTGTVP
                     EEELIAAVRENFDLRPAGIIEMLDLRRPIYKQTAAYGHFGRTDVDLPWEHTDKVDALK
                     ASLAK"
     gene            1983..3455
                     /locus_tag="IV334_001177"
     CDS             1983..3455
                     /locus_tag="IV334_001177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP8943259.1"
                     /translation="MKKETNVTLVTVSIFIATFMTAIEGTIVTTAMPTIVGSLHGMEI
                     MNWVFSIYLLTNAMLTPIYGKLADKVGRKPIFIFGTLLFIIGSLFCGLSQSMIGLIAA
                     RAIQGMGAGAMMPVALTIIGDLYSIEKRAKVLGLNSSAWGIASVFGPLAGGFIVETIS
                     WHWIFFINVPIGILLIILIWLYLNEPKIIHESKPMDIFGSFSLMAMLVSLLLGFQLLG
                     DEGMSPLVSALLLGSIVMLGIFVFAEKRAKDPVIMMHLFKNPLFVVVNLAAALASGFL
                     MGIDVYIPMWMQGVLGLNAAIGGLVLAPLSLVWMVGSFLASKWMTQMKTHWVLRIGLL
                     IAAIGGAWLYLMPFRSNYLWFFAISTVIGIGLGVTMTTSTVSAQNSVSRDQLGVATSF
                     NTLVRTIGQTVMVSIFGLLINGVTKRELAAANLTNDADIMNKLVNPQTAKYIDADILL
                     PLRKILYDGLHSVYLVGLLLVIIALVLTLFVHEKSGKIKQ"
     gene            3538..4326
                     /locus_tag="IV334_001178"
     CDS             3538..4326
                     /locus_tag="IV334_001178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase family protein"
                     /protein_id="HAP8943260.1"
                     /translation="MAFIQANIYSNVLEMEVNLNVILPQTTKKVIGTSSEQETTDIPV
                     LYLLHGMGGNHSVWERRTSIERYVANYGLAVIMPSTDLGWYTDTVYDMKYWTFIAEEL
                     PEICHELFPQLTRKREKTYAAGLSMGGYGALKLGLAKSENFAAVISLSGAVSVGNRMD
                     DLLMVRKASFWEGIFGPLDQIEGSKNDPQYLLKELVKSGKPVPRFYMACGESDFLYQA
                     NQEMVSAMQEQGLAVTFEQGPGDHNWIFWDQWIQRALAWLMEEK"
     gene            complement(4393..5061)
                     /locus_tag="IV334_001179"
     CDS             complement(4393..5061)
                     /locus_tag="IV334_001179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HAP8943261.1"
                     /translation="MEIKLSQAIIIQAAFDILAETQALSQLSMRNIANRIHVQAPALY
                     WYFKNKQQLLQIMAETMEEELALPDPTLPWNEQLLQYMENYYDLYTRFPCGAELEINT
                     VPAFPSRLEHLEQMIQLLVREGFSVTQSHQAIASLHNLLIGQLMDQQHEQQLRQKIMD
                     GNEFLKETVLFMRNYVVENQLEGFKESMQSHAELNEKALFLTSAQTYIEGLCQQNKRR
                     ENEQ"
     gene            5176..6888
                     /locus_tag="IV334_001180"
     CDS             5176..6888
                     /locus_tag="IV334_001180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP8943262.1"
                     /translation="MKLILRHAKKYKVAVFISLLSVAVMVAAALWQPKLLQQVLEAII
                     TEDNDEMKTIGIYLISLALLGLAAGVTNTIFSAKVAQGVSADIREEAFRKIQTFSFGN
                     IEQFSAGNLVVRLTNDITQIQNLVMISLQSLFRIPFLFIGAFILAMITMPQLWWIIVA
                     LIVTVFLITALSFTRMGKHFMIIQKLIDRVNSIAKENLLGIRVVKSFVQEENQLKSFS
                     KVSEDLTKHNIIVGTLFSVMIPSFMLAANLAVVGAIFFVSDLVKDDPTLIGGIASFMN
                     YLMQIMMAIIIGGMMMMMTSRAAVSLKRIAEILDAEPDLTYLDVPEQELTGSVTFDHV
                     SFRYPGEDTDTLKDISFSIKPGEMVGIVGATGAGKSTLAQLIPRLFDPTEGKVEVGGV
                     DLRQVNEKSLRQTVSFVLQKAILFSGTIAQNLRQGKKNATEKEMEHASSIAQAKEFIE
                     KLSDRYEAPIEERSSNFSGGQKQRLSITRGVIGNPKILILDDSTSALDARSEKLVREA
                     LDRDLKGTTTIVIAQKIASVVKADRILVLDEGRLVGEGTHEELVAGNRVYQEIFETQK
                     GTEE"
     gene            6891..8660
                     /locus_tag="IV334_001181"
     CDS             6891..8660
                     /locus_tag="IV334_001181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010762530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP8943263.1"
                     /translation="MTDLIKASKFFYHYLKRYKLSFFFIFITVIIATYLQVKAPQYIG
                     EAFQELANYVGGLMQGVDDKSKFVSVIWKLLLFYVLASAANFIYSILFTQVVGKSTNR
                     MRIGLFNKLEKLTIRFFDSHQDGEILSRFTSDLDNIQNSLNQALLQVITNAVLLVGIL
                     IMMFRQNVELAWATIASTPVAVLIAVLIIRKARKYVDLQQDEVGKLNGYMDEKISGQR
                     VIITNGLQEETIEGFLEHNQSVREATFKGQAYSGLLFPMMQGMSLVNTAIVIFFGGWL
                     ALNGDLERSVALGLVVTFVQYSQQYYQPLMQISSGYSMIQLAITGARRLNEIFDEKDE
                     VKPENCKAFDGVEKGLALQHVDFGYSPEKLVLKDVSINVDKGEMVALVGPTGSGKTTI
                     MNLLNRFYDVNDGSVVIDGIDIRDYDLDALRRHVGIVLQESVLFSGTIKDNITFGKPD
                     ASDEEVINAAKQANIHDFIMTLEKGYDTEISEENNLFSTGQKQLISIARTIITNPSLL
                     ILDEATSNVDTVTEARIQKAMDEAIKGRTSFVIAHRLKTILNADRIVVLRDGEVIEEG
                     SHHELLAENGFYAELYHNQFVFE"
     gene            complement(8865..9431)
                     /locus_tag="IV334_001182"
     CDS             complement(8865..9431)
                     /locus_tag="IV334_001182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HAP8943264.1"
                     /translation="MLQTALHFSHTLLQEILQPGDHVVDATMGNGHDTVFLAEHIGKT
                     GHVYSFDIQQQAIDATRERLEQRQLEERVSLFLQGHETLGEVIAEQQNLKAGIFNLGY
                     LPKSDKAIITMPETTRTAMEEILKRLVPRGRLILVVYYGHEGGEKELDMVDSFCSKLP
                     QETYNVLNYRFINQKNQPPILYCIEKKR"
     gene            complement(9431..10387)
                     /locus_tag="IV334_001183"
     CDS             complement(9431..10387)
                     /locus_tag="IV334_001183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390904.1"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HAP8943265.1"
                     /translation="MTFPYSEGNKRYHSWNYALRNEFGGKIFKVPIDGGFDCPNRDGT
                     VAHGGCTFCSVSGSGDMIVAPEDPLPIQFQKEIDMMHQKWPHVQQYIVYFQNFTNTHA
                     PVEIIKERFEQVVNLPGVVGLSVGTRPDCLPDEVVDYLAELNERMYLWVELGLQTTFE
                     ETSKLINRAHDYQTYLDGVAKLRKHNIRVCTHLINGLPGESLEMMKENVRRTILDSDI
                     QGIKLHLMHLMRKTRMLRDYHEGRLQLMSRPDYVNVICDQLEMIPKEIIIHRLTGDAP
                     WDSLIGPMWSLKKWEVLNAIDEELLRRDSFQGKYDVRKKVSV"
     gene            complement(10387..11043)
                     /locus_tag="IV334_001184"
     CDS             complement(10387..11043)
                     /locus_tag="IV334_001184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HAP8943266.1"
                     /translation="MKYKTSFQVAGSFFLVLFFILGWIVKFHLSWLSGFDQWFTGIVR
                     ASYPDLNSYQLFMTKFGNPLTVIILFACAALWMWYKKKRAETFWFVSGFIVIAGIINP
                     VIKLFFMRERPSLAHLVVETSYSFPSGHAAGSMILYGSLLFLLPAFVQKRTLRTLLQL
                     LMMFIIVSVGISRIYLGVHFPTDIIGGYLLSLSWLCLTYPVYREKRKQIDNSYRKKDF
                     "
     gene            11413..13431
                     /locus_tag="IV334_001185"
     CDS             11413..13431
                     /locus_tag="IV334_001185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HAP8943267.1"
                     /translation="MENIARKERRRLEQTKRFRKAKRGAAIVGTAMVGCSVAAPLVQP
                     VPVQADETPVQFGARINTAAFITEIATYAQPIAQANDLYASVMIAQAVVESGWGSSAL
                     SQAPYYNLFGIKGSYQGQTVYMDTLEFLNNQWVNKKEPFRQYPSFAESFNDNAYVLRN
                     TSFGNGYYYAGTWKSNTKSYTDATACLTGRYATDPGYAGKLNNIIATYGLTQYDTPAS
                     GNAGGGATTGGNNTGSAGSTGGSSNSGNTNSGSATSTTYTVQSGDSVWGISNKFGITM
                     DQLIQWNNIQNNFIYPGQKLTIKGSQENGSSTNNSGNNTNSSGNAGTSNGGQTTGAKY
                     TVQSGDSVWKISNDHGITMAQLIEWNNIKNNFVYPGQQLIVSNGGSSNAGANTGSNTG
                     NTSGSSSSSSSSSNTASGTKYTVKAGDSVWSVSNKYGITMAQLIQWNNIQNNFIYPGQ
                     QLTVSNSGSTNSTSGNTGSSTNTNNSSSVNTGSSSSQGTYTVKAGESVWSVANKNGIT
                     MDQLIEWNNIKNNFIYPGQQLIVKGGTSANTNTGSTTSAAKPNTPNTSATTSTSSTGN
                     TMYTVKAGESVWSVANKHHITMDQLIEWNNIKNNFIYPGQQVIVKKGSSQSTNQQSVT
                     GSKTYTVKSGESVWSVADSHGITMAQLIEWNNIKNNFIYPGQTLIVAK"
     gene            13858..16272
                     /locus_tag="IV334_001186"
     CDS             13858..16272
                     /locus_tag="IV334_001186"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016183651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HAP8943268.1"
                     /translation="MSYNHKEIEKKWQKYWAKTNTFNTHDDPEKPKFYALDMFPYPSG
                     QGLHVGHPEGYTATDILSRVKRSQGYNVLHPMGWDAFGLPAEQYALDTGNDPAEFTQK
                     NIETFRRQINSLGFSYDWNREVNTTDPEYYKWTQWIFTKLYEKGLAYEAEVAVNWVPE
                     LGTVISNEEVIDGKSERGGYDVVRKPMRQWMLKITAYADRLLDDLELVDWPESIKEMQ
                     RNWIGRSVGANIEFKVAGTDKSYTVFTTRPDTLFGATYSVLAPELDLVREITTPEQKA
                     AVEAYIEETAKKSDLKRTDLAKEKTGVFTGAYAINPVNGKEIPIWIADYVLSSYGTGA
                     IMAVPAHDERDFEFAQTFGLEILPVIEGGDVEKAAYTEDGTHINSEFLDGMNKQEAID
                     KMNEWLEENGVGKKEVSYRLRDWLFSRQRYWGEPIPIIHWEDGTVTAVPEEELPLRLP
                     KTKNIKPSGTGESPLANIEEWVNVVDPVTGKKGRRETNTMPQWAGSSWYYLRYIDPHN
                     KKELADYEKLKRWLPVDIYIGGAEHAVLHLLYARFWHKFLYDIGVVPTKEPFQKLYNQ
                     GMILGENNEKMSKSRGNVVNPDDVVETYGADTLRMYEMFMGPLDASIAWSENGLEGSR
                     KFLDRVWRLIVDENNKMRDRITTLNDGKLDKVYHQTVKKVTEDYENLHFNTAISQLMV
                     FINEAYKVDALPYEYIEGFVQLLAPIAPHMGEELWAILGNDGGISYAPWPTYDEAALV
                     EDEVEVVFQVNGKVRAKSNVPRDLGKDELEKAALANEIVQEYIEGKTVRKVIAVPNKL
                     VNIVAN"
     gene            16620..18173
                     /locus_tag="IV334_001187"
     CDS             16620..18173
                     /locus_tag="IV334_001187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HAP8943269.1"
                     /translation="MDQELFIIEVLSAKQKIIEKAKKRAKRIMFKLLSLVFFSGLLFF
                     GAVSFLYRHMSAVVRSAEGMQKLNNLYHFSWGVGGITIVVLIVFLLLIWLRYHQLSRH
                     FDQWLSQRAQQFLHNKPTSQDDDYLYFEKTSTSQAFRIQKSDCQLLLRSLDDLPIYLG
                     ESKSKFGMNTFHIFLLENEPRWEKSQSSPGGYLTTKMSTLVTGFFLILLAGTIYYGRY
                     TQPKMSSHINAASHMTMNSTNDTHIQKTNEGLLADPKQKVNLTTQKINDLKLDTETHS
                     LYMTTDSGANWVFVPIDSQWLRFGEYTLTTGLIPEGYWMDNTYDISADFSWFIYSSGD
                     DVRLLTSRDNGKTWQTSIITQSVQPIRYRKAVFYGNDGLFICTSASGMSAETLYIYTT
                     MDGGITWIKSGSTMINQPVQNVSFVTTSLGFVSTRESLFYTNNGGRSFKESVLSIPEN
                     YQTGGIDLFQSPNEVTQISANTLETKFYLLKKGPIDAGKMFACLFRSTDNGETWQFVQ
                     QLSQVEQGD"
     gene            complement(18554..20338)
                     /locus_tag="IV334_001188"
     CDS             complement(18554..20338)
                     /locus_tag="IV334_001188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP8943270.1"
                     /translation="MEKKKTLNTRALIRFWKMIKPEHKYFYGLFLCSLFGNLLVVAMP
                     LIMGIGIDDLLSEIRKTGIGQLSFSDIKQALLFPVLLLIGCSVLSSITSFIQERTMAA
                     LSEKITLRVRKEITKKFKTLPMSFFDNHQVGDIISRTTTGLNQLSQVLLTGINQFFTS
                     VVTIAAAGLMLFYIESKLTLLVLLLIVGSSIVTTKIANKNKQISEQNQYELGVLNNRM
                     EEILAGNLVVKTFNQQAHAEAAIHEVNKKQYNAFKRAQFMNCAIYPAIRFINQLAFII
                     SAVFGAMLVLSGGVTIGFLQAYLQYINQISEPISTASYVINSIQSAMAAIDRIFEILD
                     ETDEIPDLPDARLLEQPKGKIAFKNVQFGYTSDKILMNQVDFSVKPKQTVAIVGPTGA
                     GKTTLVNLLMRFYELNKGKIEFDGLDITKLSRGELRRSFGMVLQNTWLFEGTVADNIA
                     YGKMDATREQIIEAAEIAQCDHFIRTLPDGYDTIISSENGTLSQGQQQLLTIARVILA
                     NPPVVILDEATSSVDTRTEALIQQAMEAVTKDRTSFVIAHRLSTIENADMILVMKNGD
                     IVEKGTHSELLQSPTLYASLYNSQFQIA"
     gene            complement(20353..22074)
                     /locus_tag="IV334_001189"
     CDS             complement(20353..22074)
                     /locus_tag="IV334_001189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP8943271.1"
                     /translation="MSILKTFLQKNKGLALLTFVMICLEIAGTLGVPKLVAKLIDTGI
                     ASGEASVIKKIGVEMLLVAFLGTIAAVASSYFSALASARFGFQTREKYFRKFQQLSMK
                     DISRFSSGSLLTRMTNDVDNVQQMIVLFCQMILPAPVICVFTILMMFRYSLLLTWVTL
                     ISVVFYVWIVYRLMKRGAPLSLSIQPKMDRITITLREFFTGINMIRAFDNQSYEEKRT
                     NKSFADYANQMIKVNRIFAWVTPIAFLLMGIVYASILWFGGNLVAEGALQIGVVTAVV
                     EYSMLTLAYLMIAAMVLVIIPKSFASLRRIEEILQAEIEIEEETIKTVAEPTFDQTNA
                     AAFEHVTFKYTEISDPVLEDIDFTIPKGKTTAIVGGTGSGKSSLAKLLLRLSDPTIGK
                     VTLGGIPLTQMTQEQIRSHISYVPQKAFLFSGTILSNLQMGNEYASTEELSKAIKIAQ
                     LEELVDTLPDGLDSFVAQGGSNYSGGQKQRICIARALIKPAEIYVFDDSFSALDYKTD
                     AALRAALHKHMSEKTLVIVAQRLSTIQQADNIIVLDEGKIVGQGTHEELLSNCQTYQE
                     FAKSQGI"
     gene            22174..22935
                     /locus_tag="IV334_001190"
     CDS             22174..22935
                     /locus_tag="IV334_001190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HAP8943272.1"
                     /translation="MKKMYTIGQVSELFDLPKSTIRYWDEQGLIRSSRQEENDYRLFD
                     IDDIFMLYDIVFYRKLDIPIKQMKNLYGKTLTELYETLDETERRIHKELVVMKQKQKE
                     IREQKKQLKLMIDTNEEEFPVEEIPFDCMISTEFEDIVEIKKFLPNYSSFGMMSMPAS
                     SSQTMYGFFIDPSEVHLFTQDVIWEKKDTAVYRRFLLKSEMNHAERNNILEIRERMYE
                     KGWKTGEVIGQYLLTNTDENNIRTEYYHAWIEMKK"
BASE COUNT         7362 a         4188 c         4849 g         6914 t
ORIGIN      
        1 tggttccgtg gtgtaggggt taacatgcct gcctgtcacg caggagatcg cgggttcgat
       61 tcccgtcgga accgtttggc tcggtagctc agttggtaga gcaatggatt gaagctccat
      121 gtgtcggcag ttcgattctg tcccgcgcca ttatgcgggt gtagtttagt ggtaaaacca
      181 cagccttcca agctgttgtc gcgagttcga ttctcgtcac ccgcttttag tttttatggg
      241 cctatagctc agctggttag agcgcacgcc tgataagcgt gaggtcgatg gttcgagtcc
      301 atttaggccc atttatttta ttggcccgtt ggtcaagcgg ttaagacacc gccctttcac
      361 ggcggtaaca cgggttcgaa tcccgtacgg gtcatggaag atactttttg agtatctttt
      421 tctttttatc tgaaagaatg attttaaaaa gcttagcaga gttatcttta tggatgattg
      481 tgctaagctt ttttggacag ttctaatgta aaatattttt atatttttta agcaaaaaaa
      541 tgaaaaaata cgagcagaaa actgttttat ctgctatact tggttcgttg caagttcccg
      601 aaaggatccg caggacggag atgccttgta accgaattta actaggggga atcattataa
      661 tggtagaaag acacttattt acatcagaat ctgtttctga aggacatcca gataaaattg
      721 ctgaccaaat cagtgatgca atcttggatg caattttaaa acaagatcca acagcacgag
      781 tagcgtgtga aacgtctgta acaactggtc ttgttttagt atttggagaa atatctacga
      841 cggcatatgt agatattcaa aaagtcgtac gggaaacaat aaaagaaatt ggttatacac
      901 gtgcaaaatt cggatttgat ggagatacag cagctgtatt ggttgcgatt gatgaacaat
      961 ctcctgatat tgctcaagga gttgatgaag cacttgagat ccgggatgaa gataaaaaag
     1021 acgtattaga tgagattggt gctggtgacc aaggtttaat gtttgggttt gctgtagatg
     1081 aaacaccaga attgatgcct ttaccaatcg ctttgagtca tcgtctggta cgacgcttgg
     1141 cagatttgcg taaatcaaat gaattaacgt atttacgtcc agatgcaaaa tctcaagtaa
     1201 cggttgaata tgatgatcaa ggacaaccgg aacgcgtaga tacaatcgtt atttcaacac
     1261 agcatgatga tgcagtagat aatgaaacaa ttcgtcatga tgtcatcgaa aaagtagtga
     1321 aggaagttat tccagctgaa ttattagatg atcaaacgaa atattatatc aatccgactg
     1381 gccgatttgt cattggtggt cctcaagggg atgccggatt aacaggaaga aaaatcattg
     1441 ttgatacgta tggcggttat gctcgtcatg gtggcggtgc tttttctggt aaagatgcga
     1501 caaaagttga ccgttctgcc agctatgctg cacgttatat tgctaaaaac attgttgcag
     1561 cagggcttgc tcgcaaagca gaagtacagc tagcttatgc gattggtgtt gctcaacctg
     1621 tttcgatctc aatcaatact ttcggtactg gaacggttcc ggaagaagaa ctgattgctg
     1681 cagtgagaga aaacttcgat cttcggccag caggaatcat cgagatgctt gatttgcgtc
     1741 gtccgattta caaacaaaca gctgcttacg gtcattttgg tcgtacagat gtagatttgc
     1801 cttgggaaca tacggacaaa gtagatgcgt tgaaagcaag tttagcaaaa taagttatat
     1861 tcagctggaa cgatcgccaa gtggtggttg ttccagcttt ttttatggca gttaacctct
     1921 tgaaaaagat agtaattatt tatctatttt tagatcctgt cataatgaaa gggaggaata
     1981 agatgaaaaa agaaacaaat gttactttgg tcacagtgag tattttcatt gcaacgttca
     2041 tgacggctat tgaaggtaca atcgtaacaa ccgctatgcc aacgattgtc ggttcattac
     2101 atggaatgga aatcatgaac tgggtatttt cgatttattt gctgacaaac gctatgctta
     2161 ctccgattta cggaaaactt gcggataaag tgggacggaa accaatcttt atttttggga
     2221 cattattgtt tattatcggt tcgttatttt gcggtttatc tcaaagcatg attgggttga
     2281 ttgctgctcg tgcgattcaa gggatgggtg ctggggcaat gatgccagta gctttgacga
     2341 tcatcggcga tctttattct attgaaaagc gagcaaaagt tttaggtttg aatagttcag
     2401 cctgggggat tgccagtgtt tttggaccgc ttgcgggagg attcatcgtt gaaacgatca
     2461 gctggcactg gatctttttt atcaatgtgc cgatcggaat tctattaatt attttgattt
     2521 ggctatattt aaatgaacca aaaatcatac atgagtctaa accaatggat atttttggta
     2581 gttttagttt gatggccatg cttgtctcat tgctgttagg gttccaatta ttaggagacg
     2641 aaggaatgag tccgcttgtt tctgctttgc tgctaggtag tatcgtaatg ttaggtattt
     2701 ttgtttttgc cgaaaaaaga gctaaagatc ctgtcatcat gatgcattta ttcaaaaatc
     2761 cactatttgt tgtggtcaac ttggctgcag cattggctag tggttttttg atgggaatcg
     2821 atgtttatat ccctatgtgg atgcaaggtg tattagggct gaatgcagca attggtgggc
     2881 tggtattagc accattatca ctagtctgga tggtcggttc tttccttgca agcaagtgga
     2941 tgactcaaat gaaaactcat tgggtcttga gaattggact actaatcgca gctattgggg
     3001 gcgcttggtt gtatcttatg cctttcagaa gtaactattt atggtttttt gctatatcaa
     3061 cggtcatcgg tataggctta ggtgtgacca tgactacctc gactgtcagt gcacaaaata
     3121 gtgtatctcg tgaccagctt ggtgtagcaa cttcttttaa tacgttagtt cgaacaattg
     3181 gtcaaacagt catggtttct atttttggtt tattgataaa tggggtaact aaacgagaac
     3241 ttgcagcagc aaatttaaca aatgatgcag atatcatgaa taaactggtg aatccacaaa
     3301 cggctaaata tattgatgcg gatattttgt taccattaag aaaaattctt tatgatgggt
     3361 tacattccgt ttatctagtt ggcttgttac tcgtgatcat tgctttggtc ttaacgcttt
     3421 tcgttcatga aaaaagtggt aaaataaaac agtagtttcg gttaagatat gtacgtagcc
     3481 gaatcattca aatttaaagt tattcaaaaa taacttctta ctaaggaagg cgaaaatatg
     3541 gcatttattc aagcaaatat ttattcgaat gtgttagaaa tggaagtcaa tctaaatgtg
     3601 atactaccgc aaacaacaaa aaaggtgatt ggcaccagtt cagagcaaga gacaacggat
     3661 atacctgttt tatatttact tcatggaatg ggtggcaatc atagtgtatg ggaaagacgt
     3721 acgtcgattg aacgttatgt agcaaactac ggattagctg tcatcatgcc atctactgat
     3781 cttggctggt acacagatac agtttatgac atgaagtatt ggacattcat tgcagaagag
     3841 ctacctgaga tttgtcatga attattcccg caattaacaa gaaaaagaga aaaaacatat
     3901 gccgctggat tatcgatggg cggatatggc gcgttaaaac taggccttgc aaaatcagaa
     3961 aatttcgcag cagtaatctc tctttcaggc gctgtttctg taggaaatcg aatggatgat
     4021 cttttgatgg ttcgtaaagc atctttttgg gaagggatct ttggaccatt ggatcaaatc
     4081 gaaggttcaa aaaatgatcc gcaatactta ttgaaagaac tagtaaaaag cggaaaacca
     4141 gttccaagat tttatatggc ttgtggggaa tctgattttc tttatcaggc aaatcaggaa
     4201 atggtatctg ctatgcagga acaaggcttg gcggtcacct ttgaacaagg acctggcgat
     4261 cataactgga tcttctggga ccaatggatt caacgtgcat tggcgtggct aatggaagaa
     4321 aaataaaaaa agagtcttaa aaagatagct cctatagaga tcacaaacat agtgatctct
     4381 ataggagctt ttttactgct cgttttctct tcttttattt tgttggcata agccttcaat
     4441 ataggtttgt gcactcgtta gaaagagtgc tttttcattc aattctgcat gggactgcat
     4501 actttcttta aatccctcta attgattttc aactacataa ttacgcataa atagaactgt
     4561 ttctttcaaa aattcatttc cgtccataat tttttgtcga agctgctgtt catgctgctg
     4621 atccattaac tgtccaatca ataaattgtg gagagaggca atggcctgat gactttgggt
     4681 gactgaaaat ccttcacgaa caagtaattg gatcatttgc tccaaatgtt ctaatcgact
     4741 cggaaaagcg ggcacagtat tgatttctaa ttctgcccca caagggaaac gtgtataaag
     4801 gtcatagtaa ttttccatat actgcagcaa ctgttcattc caaggcaaag ttggatctgg
     4861 aagcgctaat tcttcttcca ttgtttcagc cattatttgc aagagctgtt gtttgttttt
     4921 gaaataccaa tataaagcag gtgcttgaac atgaattctg tttgcgatgt ttcgcatgga
     4981 aagctgcgaa agagcttgtg tttcagccag gatatcaaag gcggcctgaa tgatgattgc
     5041 ttgagaaagc tttatttcca ttttatcaac ctcgtgtatt gcattatctt tttaaacatg
     5101 gtaaattgtt agtcggtcta attttacagt gttaaattag tcctgtcaat ttaaacaaag
     5161 gggtgtaatc aacgaatgaa attaatctta cgacatgcga aaaaatataa agtagctgtt
     5221 tttatttcct tgctgtccgt agcagtcatg gttgcagcag cattatggca acctaagtta
     5281 ctacagcaag tattggaagc tattattaca gaagataatg atgaaatgaa aacaattggt
     5341 atttacctga tcagtctggc tttgctagga ttagccgcag gtgtaacgaa cacgattttc
     5401 tctgcaaaag tagctcaagg cgtaagtgca gatatccgag aagaagcatt tcgaaagatc
     5461 cagacatttt catttggaaa tatcgaacag ttctctgctg gaaatcttgt tgttcgtcta
     5521 acaaatgata tcacgcaaat ccaaaattta gtgatgattt ctttgcaatc actttttcga
     5581 attccattct tatttattgg tgccttcatt ttagctatga tcacgatgcc gcaattatgg
     5641 tggatcatcg tagcgcttat cgttacggta tttctgatta cggcactttc atttacaagg
     5701 atgggcaagc acttcatgat cattcaaaaa ctgattgatc gtgtgaatag cattgcaaaa
     5761 gaaaacttac tagggattcg tgtagtgaaa tcttttgtac aagaagaaaa ccaattgaag
     5821 tcattcagca aagtaagtga ggacttaaca aaacacaaca tcatagtcgg tacattattt
     5881 tctgtgatga tcccgtcctt catgctggct gccaatttgg ctgtggttgg ggcgatcttt
     5941 tttgtgagcg atcttgttaa agatgatcct acgttaattg gtggaattgc ttcattcatg
     6001 aactacttga tgcagattat gatggcaatc attattggcg gaatgatgat gatgatgact
     6061 tcaagagcag ccgtatcgct gaagcggatt gcggaaattt tagatgctga accagatctt
     6121 acttatttag acgttccaga acaagagttg acgggttctg tcacttttga ccatgtttct
     6181 tttcgttatc ctggtgagga tacagatacg cttaaagata tcagcttttc tatcaaacct
     6241 ggagaaatgg ttggtatcgt cggggcgact ggtgccggga agtcaacact agcacaattg
     6301 atccctcgtc tatttgatcc gacagaaggc aaagttgaag taggtggtgt agatcttcgt
     6361 caagtaaatg aaaagagttt aagacaaacg gtttcctttg ttttgcaaaa agcaatttta
     6421 ttctcaggaa cgattgctca aaacttgcgc caagggaaaa agaatgcaac agaaaaagag
     6481 atggaacatg cttcttctat tgctcaagca aaagaattca tcgaaaagtt atctgatcgt
     6541 tacgaagcac caatagaaga acgtagcagt aacttttctg gtggtcaaaa gcaacgttta
     6601 tccattactc gtggcgttat cggtaatcca aagatcttga tcctagatga cagtacgagt
     6661 gcactggatg ctcgctctga aaagctggtc cgagaagcac tcgatcgaga tttgaaggga
     6721 acaacgacga ttgtcatcgc acaaaaaatt gcttctgttg tcaaagcaga ccgcatctta
     6781 gtattggatg aaggcagatt agtcggcgaa ggaacccatg aggaattggt agctggaaac
     6841 cgtgtctatc aggaaatctt tgaaactcaa aaaggaacgg aggagtaagt atgactgatc
     6901 taataaaagc aagtaaattt ttctatcact atttgaagcg gtacaaactc tcctttttct
     6961 ttatctttat cacagtgatc attgctacct atctacaagt aaaggcacct caatacatcg
     7021 gggaagcttt tcaagaatta gctaattacg taggaggctt gatgcaaggc gtagacgata
     7081 aaagcaaatt tgtttctgta atatggaaac tcttgctgtt ttatgtacta gctagtgcag
     7141 ccaactttat ctatagtatt ttgttcacac aagtagtagg gaagtcaacg aaccgaatgc
     7201 gtatcggtct attcaacaaa ttggaaaaat tgacgatccg tttctttgat tctcatcaag
     7261 acggtgaaat tttaagtcgt tttaccagtg acttagataa tattcaaaat agtttgaacc
     7321 aagctttgct tcaagtcatc acgaatgctg ttttacttgt cgggatcttg atcatgatgt
     7381 tccgccaaaa tgtagagtta gcttgggcta caattgcttc cactccagta gccgttttga
     7441 tagctgtctt gattatccgc aaagcacgga aatatgtgga tcttcagcaa gatgaagtgg
     7501 ggaaactaaa tggttatatg gatgaaaaaa tcagcggaca acgtgtcatc atcacaaatg
     7561 gcttacaaga agaaacgatt gaaggattct tagagcataa tcaaagtgtt cgcgaagcaa
     7621 ccttcaaagg acaagcttat tctggtctac tatttccgat gatgcaagga atgtcactgg
     7681 tcaatacagc gatagttatc tttttcggcg gctggttagc attaaatggt gatttggaac
     7741 gttcggtggc attaggctta gttgtgacat tcgtccagta ttcacaacaa tattaccaac
     7801 cactgatgca gatttcatct ggatacagca tgatccaatt agcgattaca ggggcaagac
     7861 gattgaatga aatatttgat gaaaaagatg aagtcaaacc agaaaactgt aaagcatttg
     7921 atggtgttga aaaaggtctt gcgttgcagc acgtagattt tggttattct ccagaaaaac
     7981 tagtcttgaa agatgtctcg atcaatgtgg acaaaggaga aatggttgct cttgttggtc
     8041 caacaggatc ggggaaaacg acgatcatga atctgctaaa ccgtttttac gatgtcaatg
     8101 atggttcagt agtcatcgat ggaatagata tccgagatta tgatcttgat gcattacgta
     8161 gacatgtagg aatcgttttg caagaatctg tattgttctc cggaacgatc aaagacaata
     8221 tcacattcgg caaaccggac gcttcagatg aagaagtcat caatgcagca aaacaagcga
     8281 atattcatga cttcatcatg acgttagaaa aaggttatga tacagagata tccgaagaaa
     8341 ataacttatt cagtacaggt caaaagcagt tgatcagtat tgctcgaacg atcatcacaa
     8401 atccatcttt attgattttg gatgaagcaa caagtaatgt agacacggta acagaagcaa
     8461 ggatccaaaa agcaatggat gaagcaatca aaggaagaac cagctttgtg attgctcacc
     8521 ggctgaaaac aatcttgaat gcagatagaa tcgttgtctt gcgtgatggg gaagtcattg
     8581 aagaaggcag tcatcacgag ttattagcag aaaatggatt ttatgcggaa ctttatcata
     8641 atcaatttgt atttgaataa agaggttgtg aaaaagctgt cctgcatcaa gtaataagaa
     8701 cagccaatca aaaacagctt ctcatgtttt tgatttggtt ggacttatta ccgcagatat
     8761 aagcttttga gcaccgtgaa taaagaggtt atgaaatcaa cgctctaacc tgagtattag
     8821 agaaaaaaac gcaaaacata tcatttatgt tttgcgtttt ttatttatct ttttttctca
     8881 atacaatata aaataggcgg ctggttcttt tggttgataa accgataatt cagcacatta
     8941 tatgtttcct gtggcagttt ggaacaaaaa gaatcaacca tatccagttc tttttctcca
     9001 ccttcatgac catagtagac gacaaggatc aatcgtccgc gaggcacaag tcgtttcaat
     9061 atttcttcca tagctgtgcg tgtagtctct ggcatagtga tgattgcttt atcacttttt
     9121 gggagatacc ctagattgaa aatccctgct ttgagatttt gttgttcagc tatcacttct
     9181 cctaacgttt cgtggccttg tagaaacaat gagacacgtt cttctaactg acgctgttct
     9241 agcctttcac gagtagcatc gattgcttgt tgctggatat caaaactata gacatgacca
     9301 gtttttccga tgtgttctgc tagaaaaacc gtatcgtggc cattgcccat cgttgcatct
     9361 acgacgtgat cccctggttg caagatttct tgcagtaatg tatggctgaa atgcaaagca
     9421 gtttgcaaca tcagacgctc acctttttcc gtacatcata ttttccttgg aagctatctc
     9481 tgcgaagaag ttcttcatca attgcattca agacttccca ttttttcaag ctccacattg
     9541 gtccaatcaa ggaatcccac ggcgcatccc ctgtcaaacg atggatgatg atctcttttg
     9601 gtatcatttc cagctggtcg caaatgacat tgacatagtc aggacggctc attaattgaa
     9661 ggcgaccttc atgatagtcc cgcaacattc tcgttttgcg catcagatgc atcaaatgga
     9721 gcttgattcc ttggatatct gaatctaaaa tggtgcgtct gacattttct ttcatcattt
     9781 ctaaactttc gcctggtaat ccgttgatca aatgcgtaca aacacggata ttatgtttgc
     9841 gtaatttagc cacgccatct aaataagttt gataatcatg tgctcggttg atcagttttg
     9901 aagtttcttc aaaagttgtt tgcaatccaa gttctaccca taaatacatc cgctcgttca
     9961 gttcagcaag gtaatcaact acttcatctg gcagacaatc cggtcgtgta ccgactgata
    10021 atcctacgac acctggtaaa ttgactactt gctcaaatcg ttctttgatt atttcaactg
    10081 gcgcatgggt gtttgtaaaa ttttggaaat aaacgatgta ttgttggaca tgaggccatt
    10141 tttgatgcat catgtcgatt tctttctgga attggattgg aagagggtct tcaggtgcaa
    10201 cgatcatgtc tcccgaacca gatacactgc aaaaagtaca accgccatgg gcaacagtcc
    10261 catctcgatt cggacagtca aatcctccat cgatcggcac cttgaaaatt ttcccgccaa
    10321 actcatttcg taaagcgtaa ttccaagaat gatatctttt atttccttct gaataaggaa
    10381 aagtcattaa aagtccttct ttctgtaaga gttatctatt tgttttcttt tttctctata
    10441 cactggataa gtcaggcaca gccaagaaag actgagtaag taaccgccga tgatatcagt
    10501 aggaaaatga acgcccagat aaatgcggct tatcccgaca cttactataa taaacatcat
    10561 cagcaattga agcaatgtgc gtaaggttct tttttgaaca aaagctggaa gcagaaatag
    10621 taaagaaccg taaagaatca tactgcctgc tgcatgtcca ctagggaaac tgtaacttgt
    10681 ttcaactact aagtgcgcca atgaaggacg ctctcgcatg aaaaatagtt tgatcaccgg
    10741 gttgatgatt ccagcaatta caataaatcc agaaacgaac caaaatgttt ctgccctttt
    10801 tttcttatac cacatccata aagccgcaca agcaaataaa atgatgacgg taagaggatt
    10861 accgaattta gtcataaaga gttggtagct gttcagatca ggataagaag ctctgacaat
    10921 gcctgtaaac cactgatcga aaccagaaag ccacgacaga tggaacttta cgatccagcc
    10981 caaaatgaaa aacaggacaa gaaagaagct accagccacc tgaaatgatg ttttatattt
    11041 catacaattc ctcctttgaa gcaatgactt attatagcat ttttggtcca gcgaaaaaaa
    11101 cggtaaagag ataaaaccaa tgtatgattc ctctttgaat aaccaatatt cttataaaaa
    11161 aaataactgt aatcggaaag acattttttg gtaatgcaaa tgaaatgtta gggaaataaa
    11221 atcagtgttt ttcgaaatcc ttttaaacaa gaacgtagac gtaggaagaa cttgaaaatg
    11281 aacagaagtt gttcattacg gtaaattaca atatgattac aaaaaacaac aaagctctta
    11341 aataactttt aataaatatt aagagaagtt ataattatta ataatagata aattgaagtt
    11401 ggaggataga agatggaaaa cattgcaaga aaagaacgga gacgtttaga acagaccaaa
    11461 cgttttcgaa aagcaaaacg aggggcagca atcgtgggaa ctgcgatggt aggctgttct
    11521 gtcgctgccc cattagtaca gccggttcct gttcaagcgg atgaaacacc tgttcagttc
    11581 ggcgcaagaa tcaatacagc ggcatttatc acagaaattg ctacttatgc tcaaccaatc
    11641 gctcaagcca acgatctata tgcatcagtg atgatcgcac aagcagttgt agaaagcggt
    11701 tggggcagta gtgcgttatc tcaagcacct tattataatc tttttgggat aaaaggcagc
    11761 tatcaaggac aaaccgtcta catggatacg cttgaatttt taaataatca atgggtaaat
    11821 aaaaaggagc ctttccgaca atatccttct tttgctgaat ctttcaatga caatgcctat
    11881 gtacttagaa atacttcatt tggcaacggt tattactatg ccggtacttg gaagagcaac
    11941 acgaaatcct atacagatgc gacagcttgt ttgacagggc gttatgcgac tgatcctggt
    12001 tacgcaggca aattaaataa tatcatcgca acttacggat tgacccaata tgatacacca
    12061 gctagcggaa acgcaggcgg tggagcgact acaggtggca ataatactgg aagtgcaggt
    12121 agtactggcg gttcaagcaa ttctggtaat acaaactcag gttcagcaac ttcgaccact
    12181 tatactgtcc aatctggtga ttctgtttgg ggaatctcaa ataaattcgg tatcacaatg
    12241 gatcaattga tccaatggaa caacatccaa aacaatttca tttatccagg acaaaaactg
    12301 acaatcaaag gtagccagga aaatggctct tcgacgaata attccggtaa caataccaat
    12361 tcttcaggaa atgctggtac atcaaacgga ggacaaacaa ctggtgcgaa atacactgtt
    12421 caatctggtg attctgtttg gaagatctca aatgatcacg gaattacgat ggctcaattg
    12481 attgaatgga acaatatcaa gaacaatttc gtttatccag gacaacagct aatcgtatca
    12541 aatggcggtt cttctaatgc tggagcaaat actggctcaa atactgggaa tacaagtggc
    12601 tctagttcgt ccagctcaag ttcttctaat acagcttcag gaacgaaata tacagttaaa
    12661 gccggagact ctgtttggag cgtctcaaat aaatacggaa ttacgatggc tcagctaatc
    12721 caatggaaca atatccaaaa taactttatc tacccaggac aacaactgac tgtttcaaac
    12781 agtggatcaa caaacagcac gtcaggtaat acaggttcgt caaccaatac aaataacagc
    12841 agttcagtta atactggcag cagttcgtct caaggcactt ataccgtgaa agcaggtgaa
    12901 tctgtttgga gcgtggcaaa taaaaacggc atcacgatgg atcagttaat cgaatggaat
    12961 aatatcaaaa acaacttcat ttatccagga cagcaattga tcgttaaagg tggaacttcg
    13021 gcaaatacaa atacaggttc aactacttct gcagcgaaac caaatacacc gaatacgtct
    13081 gctacgacaa gtacatcatc cactggtaat acgatgtata ccgtgaaagc gggcgagtca
    13141 gtttggagcg tagcgaacaa acatcatatc acgatggatc agttaatcga atggaataat
    13201 atcaaaaata atttcatcta tccaggacag caagtgattg tgaagaaagg ttcatctcaa
    13261 tcaacaaacc aacaatcagt tactggttcg aaaacctata ctgtgaaatc tggagaatct
    13321 gtttggagtg ttgctgacag tcatggaatt acaatggctc aactaatcga atggaacaat
    13381 attaagaaca actttattta tccaggtcaa acgttgattg tagctaaata aaatagctgt
    13441 tcgaagaaat aacgattgaa ttccacaaaa atatttgtta ctataaggct acttaaatat
    13501 gacaacactt gaaaaggaag tagtaaaaag actgaagttt cagagagcgc atggctggtg
    13561 agaatgtgca cggatgcttt tgaactcgcc tttgagttgc ccttgctgaa tacccgagta
    13621 agcaagcggc ggtgacggcc gttacaacgt tcgaggcctt ttcactaagg taaaattagg
    13681 tggtaccgcg tagattacgt cctataaaaa gagcaatctt tttataggat ttttttattt
    13741 cttttgtgaa gtttgagaaa acaaagcaaa aggaaataaa aataaacaga ttctgccggt
    13801 tttttcgtta tatttataat agaaacaagt gttttcaata gaacaggagg acagcaaatg
    13861 agttacaatc ataaagaaat tgagaaaaaa tggcaaaaat actgggcaaa aacgaatact
    13921 ttcaacaccc atgatgatcc tgaaaagcca aaattttacg cactggatat gtttccctat
    13981 ccatccggtc aagggctaca tgtagggcat cctgaaggtt atacggcaac ggatatcctt
    14041 tcacgtgtga aaagaagtca aggatacaac gtgctgcatc caatggggtg ggatgcattc
    14101 ggtctgcctg ctgaacaata tgcgctagat acaggaaacg atccagctga gtttacccaa
    14161 aaaaatatcg aaacattccg acgccaaatc aattctttag gatttagtta tgactggaat
    14221 cgcgaagtga atacaactga tccggagtat tataaatgga cacaatggat ctttacgaag
    14281 ctttatgaaa aaggtctagc ttatgaagca gaagtagcgg taaactgggt acctgaatta
    14341 ggaacagtta tttcaaatga agaagtgatc gatgggaaaa gtgaacgtgg tggctatgat
    14401 gtagtcagaa aaccaatgcg ccaatggatg ctgaaaatca ctgcttatgc tgatcgctta
    14461 ttggatgatc tagaattagt tgattggcca gaaagcatta aagaaatgca aagaaattgg
    14521 atcggtcgtt cagtaggagc aaatattgaa tttaaagtgg ctggtacgga taaatcctat
    14581 accgtattta cgacacgacc agatactcta ttcggtgcaa cttactcggt tcttgcacct
    14641 gaactagatc ttgttagaga aattacgacc cctgaacaaa aagctgctgt agaagcttat
    14701 atcgaagaga cagctaaaaa atcagatcta aagagaacgg atcttgcaaa agaaaaaaca
    14761 ggagtattta ctggtgctta tgcaatcaat ccagttaatg gcaaggaaat cccaatctgg
    14821 atcgccgatt atgtattatc ttcatacggc acaggagcca tcatggctgt tccagcacat
    14881 gatgaacgtg actttgaatt cgctcaaact ttcggactag aaattcttcc agttatcgaa
    14941 ggtggagatg ttgaaaaagc tgcttacaca gaagatggta cgcacatcaa ctctgaattt
    15001 ctagacggca tgaacaaaca agaagcaatc gataaaatga atgaatggct agaagaaaat
    15061 ggcgtaggga aaaaagaagt cagctatcgc ttacgtgact ggctattctc ccgtcaacgt
    15121 tactggggtg aaccaatccc aatcatccat tgggaggacg gaacagtcac agctgtccca
    15181 gaagaagagc taccattacg gttgcctaag acaaaaaaca tcaagccaag cggtactgga
    15241 gaatctccat tagcaaatat cgaggagtgg gtcaatgtcg ttgatcctgt aacaggtaaa
    15301 aaaggccgtc gcgaaacgaa tacaatgcca caatgggcag gaagctcatg gtattacctg
    15361 cgttatatcg accctcataa caaaaaagaa ctagcagatt atgaaaagtt gaagcgttgg
    15421 ctaccagttg acatctacat tggtggagca gaacatgcgg tacttcattt actatatgca
    15481 cgattctggc ataaattctt atacgatatc ggtgttgttc ctacaaaaga accattccaa
    15541 aaactataca atcaaggaat gatcttaggc gaaaacaacg aaaaaatgtc aaaatctcgt
    15601 ggtaatgtag tcaatcctga tgatgtggtc gaaacttacg gtgcagatac actgcgcatg
    15661 tacgaaatgt tcatggggcc attagatgct tcgattgctt ggagcgaaaa tggcttagaa
    15721 ggaagccgta aattcttgga tcgtgtatgg cgtttgatcg ttgatgagaa caataaaatg
    15781 cgtgaccgta tcactacatt gaatgatggc aagcttgata aagtgtatca tcaaacagtg
    15841 aaaaaagtga cagaagacta tgaaaatctt catttcaata cagcaatctc ccaattgatg
    15901 gtctttatca atgaagccta taaagtggac gctctgcctt atgaatatat cgaaggattt
    15961 gttcaactac tcgcaccgat tgctcctcac atgggtgaag aactatgggc aatccttgga
    16021 aatgatggtg gtatttctta cgcaccatgg ccaacatacg atgaagcagc acttgttgaa
    16081 gacgaagtag aagttgtttt ccaagtaaac ggtaaagttc gtgcgaaaag caatgttccc
    16141 cgagacttag gaaaagatga gctagaaaaa gcagcattag ccaatgaaat agtgcaagaa
    16201 tatattgaag gcaaaacagt cagaaaagtt attgcggtac caaacaagtt agtcaatatc
    16261 gttgctaatt aattggctct ttgtcaataa ggactgatga gttgtgcaaa atcaaatctg
    16321 ggccagaatg aacttcattc tgtctcagat tttttgttat ttcaaattga ttaattgatt
    16381 gatcaaaaag gttttgattt tcatgttctc aaacacgttc aatgtctttt tcttctgaat
    16441 ggtcttgtaa gagatctctg taatcatcaa atttttgtct attaatctta gtattttttg
    16501 atgtataaac tacctgaaat tgagagtaca taccaaattc ataaattttt tattagtggg
    16561 actagtgatt gtatggattt tgacgcataa tgaatgaagt atgagaaaag gagagacaaa
    16621 tggatcaaga actatttatt atcgaagtat tatcagctaa acaaaaaatt atcgagaaag
    16681 cgaaaaaaag agcgaaaaga ataatgttca agcttttaag cttagtattt ttctctggtt
    16741 tgcttttttt cggagctgta tcgttcctat atcgccatat gagtgcagta gtccgttcag
    16801 ctgaagggat gcaaaaatta aataatttgt atcacttttc gtggggagtt ggcggaataa
    16861 caattgttgt gcttattgtt tttcttctac ttatttggct ccgttaccat caattgtcgc
    16921 gtcatttcga tcaatggctt agtcaaagag cacaacagtt tttacataat aaaccaactt
    16981 ctcaagatga tgactatctt tattttgaga agacttcgac gagtcaagct tttcggatac
    17041 aaaaaagtga ttgccagtta ttgctgagat cacttgatga tttacctatc tacttaggcg
    17101 aaagcaaatc aaaattcgga atgaacacat tccatatttt tttactagaa aacgaaccca
    17161 gatgggaaaa aagccagtct tcgcctggtg gatacctgac aactaagatg agtacattag
    17221 tcactggttt tttcctcatt cttttagctg gaacaatcta ttatgggaga tacactcagc
    17281 caaaaatgtc ttctcatata aatgctgctt ctcacatgac aatgaactca actaacgata
    17341 cgcatataca aaagacaaat gaagggttgt tagctgatcc taagcaaaag gtaaatttga
    17401 caacacaaaa gataaatgat ctcaaacttg atacagagac gcactctctc tatatgacaa
    17461 cagatagtgg tgctaattgg gtatttgtgc cgattgattc gcaatggttg cgttttggcg
    17521 aatacaccct gacaacgggg ctgattcctg aaggttattg gatggacaac acatatgata
    17581 tttccgccga tttctcatgg tttatctatt cttctgggga tgatgtacgt ctgctgactt
    17641 caagagataa tggtaaaaca tggcaaacgt ctattatcac ccaatcagtt cagccgatac
    17701 gttatcgaaa agcggtattt tatggcaacg atggtctatt tatctgtaca tcagccagtg
    17761 ggatgtctgc agaaactttg tacatttaca ctactatgga tggcggaatc acttggataa
    17821 aaagtggaag tacaatgatc aatcagccag tacaaaatgt cagcttcgtt acaacttctt
    17881 taggttttgt ttcgacacgt gaaagtttgt tttatacaaa caacggagga cgctcattca
    17941 aagaatcagt tctgtcgatt ccggaaaact atcagacagg ggggatagat ctttttcaat
    18001 caccaaatga agttactcaa ataagcgcga atacgttaga gacaaaattt tatttgttga
    18061 aaaaaggacc aatcgatgca gggaaaatgt ttgcctgcct cttccgttca actgataacg
    18121 gggaaacatg gcaattcgtt cagcaactat cgcaagtaga acaaggagat taaagaagag
    18181 agattgtgag cctgacgttc agcaccgagt cacaagaaac aaattcaaaa aacagcttct
    18241 catatttgtt gaatagtgta actcgtgatt cggagttgac aagagcaaca agaagcgatt
    18301 ttgaagtata gcttctcgta ttttacaaga tcgtgtctta ttgccgatag gttgtctctt
    18361 gaacaccgtt tattcgtaaa aagggattgt gacaagtctt ttgtcactac ttaccgaacg
    18421 aaactgaagg agctatccaa aaagtatcat tagtattatt ctttgtgttt ctagtggcac
    18481 cactagtaca tttaaaaaag ctatcgatca tggactaatc atacatgatc gatagctttt
    18541 tagataatgt ttgttacgct atctgaaatt gactattata aagactggca taaagcgttg
    18601 gtgattggag tagttcagaa tgtgttcctt tttcgacgat atcgccattc ttcattacaa
    18661 ggatcatgtc tgcattttca atcgttgaca aacggtgtgc aatcacaaaa cttgtcctat
    18721 cttttgtcac agcttccatt gcttgctgaa tcaatgcttc tgttcgtgta tctacgctag
    18781 aagttgcttc atctaaaata acgacaggag gattcgctag aatgactcga gcaattgtca
    18841 ataattgttg ctgtccttga gataacgtgc cgttttcgct agaaatgatc gtatcatatc
    18901 cgtctggcag tgttcgaata aagtgatcac attgtgcgat ttcagccgct tcgataattt
    18961 gttctcttgt ggcatccatt ttcccgtagg cgatattatc agctaccgtt ccttcaaaca
    19021 gccaggtatt ttgtaaaacc atcccaaaag aacgacgtag ttctccacgt gaaagcttgg
    19081 taatatcaag accgtcgaat tcgatctttc ctttattcag ttcataaaaa cgcatcagca
    19141 gattgactaa agtcgttttt cctgcaccag ttggaccaac gatcgctacc gtttgttttg
    19201 gctttactga aaaatcgact tgattcatca atattttatc tgatgtgtaa ccaaactgta
    19261 catttttgaa agctattttt cctttcggtt gttctaaaag tcttgcatct ggaaggtctg
    19321 gaatttcatc tgtttcatct aatatctcaa atatcctatc gattgctgcc attgcggatt
    19381 ggatcgagtt gatcacataa gaagcggtag aaattggttc agaaatctga ttgatgtact
    19441 gaagataagc ttgtaagaag ccgatcgtca caccacctga tagaacaagc atagcaccga
    19501 ataccgcact tatgatgaag gcgagttgat taatgaaacg aatggccgga tagatagcac
    19561 agttcataaa ttgagcacgt ttaaaagcgt tgtattgctt tttatttact tcgtgtatag
    19621 cagcttctgc atgagcttgc tgattgaatg ttttgaccac tagattacca gctaagattt
    19681 cttccattcg attatttaaa acacccagtt catactgatt ctgttcagaa atctgtttgt
    19741 ttttgttcgc aatcttagtt gtaacaattg aactgccaac gatcagcaat aagacgagca
    19801 aagtaagctt gctttcgata taaaacagca tcagaccagc agcagctatt gttacgacag
    19861 aagtaaaaaa ttgattgata cctgtcaata acacttgaga aagttgattg agaccggtag
    19921 ttgtccgact aatgatgtca cctacttgat gattgtcaaa aaacgacatc ggtaatgttt
    19981 tgaatttttt tgtgatttct tttcgcaccc tcaatgtgat cttttcgctt aacgctgcca
    20041 ttgtccgttc ctgaataaat gaagtgatgc tgcttaagac tgaacagccg atcagcaata
    20101 agacagggaa caacaatgct tgtttgatat cactgaaaga aagctgtcca atacctgttt
    20161 ttcttatttc tgataagaga tcatcgatcc cgattcccat gatcaagggc atggctacga
    20221 ctaataggtt cccgaataaa ctgcatagaa acaatccgta aaaatattta tgttcaggtt
    20281 tgatcatttt ccaaaaacgt atcaaagcac gcgtgttcaa cgtttttttc ttttccatcg
    20341 tatcccttct ttctatattc cttgtgattt tgcaaactcc tgataagttt ggcaattaga
    20401 cagtagttct tcatgagtac cttgtccaac gatttttcct tcatctaaaa caatgatgtt
    20461 atctgcttgt tggattgtac tgaggcgttg agcaacaatc acgagagttt tctccgacat
    20521 gtgtttatga agggctgctc ttaatgctgc atctgtttta tagtctaaag cggagaaact
    20581 atcatcgaat acatagatct ctgctggttt gatcaacgct cgagcaatac atatccgctg
    20641 tttctggccc cctgaataat tagaaccgcc ttgagcaaca aagctgtcca atccgtcagg
    20701 aagggtgtcc actagttctt ctaattgagc gatcttgatc gctttagaca actcttctgt
    20761 tgaggcatat tcatttccca tctgcaagtt gcttaaaatt gtcccgctga ataagaaagc
    20821 cttctgaggg acataactga tgtgggagcg gatctgttct tgagtcattt gagtaagagg
    20881 aataccacca agggttactt ttcctatcgt agggtcacta agtctcaaga gaagtttcgc
    20941 caaactactt tttccagatc cggtcccgcc aacgatcgct gttgtttttc ctttaggtat
    21001 tgtgaaatca atatcttcta gaactggatc agagatttca gtatatttaa atgtgacatg
    21061 ttcaaaagca gctgcatttg tctgatcaaa ggttggttca gctactgttt tgatcgtctc
    21121 ttcctcaatt tcgatctctg cttgcagtat ttcttctatc cgtcgtaatg aggcaaacga
    21181 tttagggata atgaccaata ccatagctgc gatcatcaag taagcaagtg tcagcataga
    21241 gtattcgact acagcagtca cgacaccgat ttgtaatgct ccttctgcta caagatttcc
    21301 gccaaaccac aaaatagaag cataaacgat tcccatgagc aaaaaagcaa ttggagtgac
    21361 ccaagcaaat attcggttca ctttgatcat ttgattagca tagtcagcaa agctcttatt
    21421 cgttcgcttt tcttcatatg attgattatc aaaggcacgg atcatattaa tcccagtaaa
    21481 gaattctctc aatgtgatgg tgatccgatc catttttggc tggatactca aagataaagg
    21541 tgcccctcgt ttcatcagtc gataaacaat ccatacataa aaaacaacag agatcaatgt
    21601 aacccatgtt agtaatagag aataacgaaa catcatgaga atggtgaaaa cgcaaataac
    21661 cggtgctggt aagatcattt ggcaaaataa aacaatcatt tgctggacat tatcgacatc
    21721 attcgtcatt cttgtcaaaa gagatccaga actaaaacga ctgatatcct tcattgacag
    21781 ctgttggaat ttacggaaat atttctcccg cgtttggaat ccaaacctag cggaagccaa
    21841 tgctgaaaag taactagagg caactgcagc gattgttcct agaaacgcta caagcagcat
    21901 ttcaactcca atttttttta taactgaggc ttcgccactt gctatacctg tgtcgatcag
    21961 ctttgctact aattttggta cacctaatgt accggcgatt tctaaacaga tcatcacaaa
    22021 ggtaaggaga gcgagtcctt tgtttttctg taagaatgtt tttaagatag acatattatt
    22081 cctccttttt tcctgatact tttcgattct ataccttcaa gtaacttgaa ggtcaataga
    22141 aaaaagaata aactgaaaaa gaggtgagaa aaaatgaaaa aaatgtatac gattggccaa
    22201 gtatcggaac tatttgattt gccaaaatca acgatccgct attgggatga acaaggatta
    22261 atccgttcaa gtagacaaga agagaatgat tatcgattgt ttgatatcga tgatattttt
    22321 atgctttatg atatcgtttt ttatagaaaa ttagatatac cgatcaaaca gatgaagaat
    22381 ttatatggaa aaacattgac ggagttatat gagacacttg atgaaacaga aagaagaatc
    22441 cataaagaac tggtggtgat gaaacaaaaa caaaaagaga taagggagca aaaaaaacag
    22501 ttgaaattga tgatcgatac aaacgaggaa gaatttcctg tagaagagat cccttttgac
    22561 tgtatgatct caacggagtt tgaagatatt gtggaaatca aaaaatttct acctaactat
    22621 tcaagctttg ggatgatgag tatgccagct tccagttcac agacgatgta tggatttttt
    22681 atcgacccta gtgaagtaca tttatttact caagacgtga tttgggagaa aaaagatacg
    22741 gctgtttatc gtcgattctt attgaaatcc gagatgaacc atgcggaacg taataatatt
    22801 ttagaaatca gagaaagaat gtatgaaaaa ggatggaaaa caggagaagt gatcggccag
    22861 tatctattga cgaatacgga tgaaaacaat atcagaactg aatattatca tgcatggata
    22921 gaaatgaaaa aataggaaat atgtgtttaa gataactcga aaatgatggt tgttttagtt
    22981 ataaagaaaa attttctggt tcgattacgg gtttaatgtg ggggattatt agtcgtggat
    23041 agatagaatt ttagtttttt agaggaaata tgtctttaaa aatggaatgg ttgcaattct
    23101 tagcgatata tacgatgtaa ttgtcctctt aaaaaagtat ggaaaaataa ttgtattggg
    23161 tgttatgtat aattcatggt gaactgagac taatgagaag aaaaattcat attgttatga
    23221 gtgagattgt atcagatgag aatgataaat taaataggtg cataaaagca ttacacttaa
    23281 aatacattgt tgcttacatt attacaatat gtt
//
LOCUS       DACDKM010000031        23209 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20834_2016
            SAMN12640370-rid9891663.denovo.031, whole genome shotgun sequence.
ACCESSION   DACDKM010000031 DACDKM010000000
VERSION     DACDKM010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640370
            Sequence Read Archive: SRR10040891
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23209)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23209)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742835.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742835.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:04:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,012
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 29 of 75
            Pseudo Genes (incomplete)         :: 33 of 75
            Pseudo Genes (internal stop)      :: 19 of 75
            Pseudo Genes (multiple problems)  :: 5 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23209
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640370-rid9891663.denovo.031"
                     /isolate="E20834_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..637
                     /locus_tag="IV334_001191"
     CDS             <1..637
                     /locus_tag="IV334_001191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP8943273.1"
                     /translation="FAIIMSELRNKRLVKVYQTMSLLPYFLSWVIISYFVYAFLSPDK
                     GIFNQWITGHGGEAVNWYNEPKYWPFILVFIGTWKGIGYNSIIYFASVMGIDPTYYEA
                     AMVDGASKWQQIKHVTIPQLIPLMTILTILAVGNIFRADFGLFYNIPRNSGALYDVTQ
                     VLDTYIYNGLTSTGDFGMTAAAGLYQSVVGFVLLMITNTIARRFDSDSALF"
     gene            649..1569
                     /locus_tag="IV334_001192"
     CDS             649..1569
                     /locus_tag="IV334_001192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAP8943274.1"
                     /translation="MKKKKVERVQVRSFNKTANFFSNIMIALFAISCILPFIFVIAIS
                     FTDESILSTHGYSFWPEKFSIFGYTYLFDQMQDKIFQALFVTVLVTVLGTLINSTATS
                     LYAYAISRSNFPFRRFFTVFCLITMLFSPGMVANYLVMTNLLQLKDTIWALILPMAVS
                     PFNIIVMRTFFKRSVSDSIIESARIDGASELRIFVQIVLPLAVPGIATISLFAALGYW
                     NDWFNALLYIQDDKLVPLQYLLMKIQSNIQYLTQNSGAGSQLTGGLASVPGESARMAI
                     VVISTLPIAISYPFFQKHFVKGLTIGGVKE"
     gene            1610..3067
                     /locus_tag="IV334_001193"
     CDS             1610..3067
                     /locus_tag="IV334_001193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAP8943275.1"
                     /translation="MKLWKKLAFTGVSALLLGTLAACGGSGSKDQAEASNSDTLQMYQ
                     IGDKPDNFDQLMEVANKRIEKEIGVKVNINYIGWGDYEKKMNVIISSGENYDIAFANN
                     YVSNAQKGAFADLTELAPKYAEKAYNDLDEAYIKGNLVNGKLYAFPVNGNVFAQQVLT
                     FNKALLDKYDLSIDGIQSYADAEKVLKEFHEKEPNTAAFAIGQGFKVQGNFDYPLGNT
                     LPFAIDLDGDDSKIINQYDNETYIGLLRTMHSWYKQGLIPSDAATSNKDYPLEGNTWL
                     MRGETQGPYDYGDTILTNAAKQELVSKAITVPLKSTAQAQMANFVVSNTSKNKEKSVE
                     LLGLLNSDPELLNGLVWGIEGEAWEKVDGSDDKIKLLDGYQPNTHMSAWNTGNNKILY
                     TQESITDDMIAKRDQSIADAETSPILGFSFNTDSVKTELSNISNVMNQYLDGLNTGTV
                     DPDETLPKLKDALDKAGYDKVLKEMQKQYDEFRQE"
     gene            3412..4026
                     /locus_tag="IV334_001194"
     CDS             3412..4026
                     /locus_tag="IV334_001194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HAP8943276.1"
                     /translation="MLGKVLEELFIRIWVIIKLTLYFWIYTFAGGIIFGLGAAWKTVN
                     ELFYLYGFEYKEITIKRGWNIYKRNFLRGNLLFSLFLSGTALLSYNLYLSVQIKGLIF
                     LAIDFILLFALLCLFATYQYSMILDSEYTISVPNLLKLSFISVFSSFSSFLKIIIGSG
                     IILGVTWQFKGLILFGVIGLLTVWNGTMTTHWREELDKQLESYE"
     gene            4019..5743
                     /locus_tag="IV334_001195"
     CDS             4019..5743
                     /locus_tag="IV334_001195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HAP8943277.1"
                     /translation="MNKFRILFSKGTLLNRLLRKYAVIMIGVTMTATVIFSIHTWEQN
                     QKQAENMTSDAVQSTSRMLNDKTTLSRIIKNQLVGNSEKIENVTTYLTKPIDQYLMYV
                     YEQQNSTDELVSFPNQIKDLYANYEELSAIYIVLNQLPEYYESSRENKGGEIKKGTPK
                     LLDAFYIKLPITQGGAESGSIFIGFQKKELDMILENLTSFNGLSLYMISGTTNRLYTF
                     HDNKIAKETFERQEMIITESLKKNSQLPIDDLEKNNFVQHQELSDDYRILAVLDKKSV
                     KIETGKNLAPLIIGIIILDNILLIFLYKTFKRYSQQVSIVMQAMDQTAAGNLETRIDI
                     TNTEYELRELSIGINEMLDSINQFVEDIYKLEIKQQDAHMRALQAQINPHFLYNTLEY
                     IRMYAISEGSEELADVVYAFSALLRNNTNQEKTITLKEELDFCEKYVYLYQMRYPNRV
                     AYHFMIDPDLEKIEVPKFVIQPLVENYFKHGIDFTRFDNALSVKVLQEGKRVRIIIKD
                     NGKGMTEKRLKQIEEKLSHPKVELHGSIGLQNVNERLRASFGSSYYMSLENNETGGLT
                     VSITFKEG"
     gene            5740..7191
                     /locus_tag="IV334_001196"
     CDS             5740..7191
                     /locus_tag="IV334_001196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP8943278.1"
                     /translation="MSVYQVLLVDDEYMILNGLKKIIDWQSLGFQIVATAENAKEGLA
                     VLEQRQIDLVVTDVTMPEINGLEFIEAAQKERHNFEFMILSGYQEFDYLKGGMQLGAV
                     NYLMKPVNKFELVESLKKIKTRLDQQNEQKNQQEIYQELLFSQWLNEELDEISEEEII
                     GQISEKQHRIVLIQLARTHDTLINRWLQERQQIFYYQRNYGDRVLITLLLEADEVDGF
                     CCFVQENFPDQEWLISIGEETDSLDKIPETFQQAKDNLQLHQFYGDKDEHVFYAVQAE
                     TKDQTIDFSSFRRVLQNKQLEVAQKMITDFFEQFQLAAMMPEDIRYSSFLLFMEIQRE
                     LIDLEDEEYLQGIEKIHQAKTVQELQQLLLSFIKKHQRQKKYSDNVEKVIVILHQHYQ
                     EPLTLKEVSENLHLNVMYLGQLFKKETKKSFSAYLNHLRMEKAKQLLLHSNQNINEIA
                     SEIGYNNTTYFSKLFKKIVGRSPKEYRENSGES"
     gene            complement(7664..9817)
                     /locus_tag="IV334_001197"
     CDS             complement(7664..9817)
                     /locus_tag="IV334_001197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HAP8943279.1"
                     /translation="MNIGQIDTRHATANQPNFSNGNCQPYTGVPFGMNYFAPQTTDQN
                     GSWWFHPDDRVFQGYRLTHQPSPWMGDFSHMLFTPINGKLQENTLFHAQSSYRPEESI
                     FCPTHLSICQLRYGICSTLIPSMYGGILSIDYSKNDSGLLLSFPGRHQLFVIDPYTVE
                     GKITHFAGSEDPDFSFYFILRFEYPLTIDELAVSNKNSDSIPLYFEQTKKQTIRFGTS
                     FISSEQAAYNLELEQNQSKETYLAHSQEQWQSYFDRIQLTHHDQQQLRTFYHNLYRVF
                     LFPQTFYEIDPSGQKIHYDTTSRSIKPGVLYTNNGFWDTYKTVYPLYSLIAVEKYEEM
                     LEGFLNSYRESGYLPKWLSPDERGLMPGTLIDAVIADAAVKDIRRDLMPEFLTAMEKG
                     ATVQSKHPNYGRQGTEDYLTYGYVPNHYHESVNHTLDYAYSDFCISQVAKVLEEEEMT
                     KKYQQQSLNYQNIFDYESGFMRSKDKDGKFREPFSANRWGQDYAEGSAWQSSFAVYHD
                     FSGLIGKYGGSERYKEKLIELCNQPPTFDPGGYGFEIHEMSEMAAIEFGQLAISNQPS
                     FHYPYLFSYIGQPEMAQPLLKQLMTQTFDDSPMGYPGDEDNGSMAAWYIFNSLGFYPV
                     TPGTGEYVIGMPLIDKAVLLLSNGKELTIEATPNRPQQQFIHKIKRMDREYTKLYFTH
                     QDLLKGGTISYQLGIIPHPKKYQAKDLPFSLSNYN"
     gene            complement(9819..10865)
                     /locus_tag="IV334_001198"
     CDS             complement(9819..10865)
                     /locus_tag="IV334_001198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAP8943280.1"
                     /translation="MTQPLYQTIFNDLQTAILTGELPVDSQLPTEKELSRTYKVSRIT
                     SKRALTELEQLGLIYRVRGKGSFVKSASPEQILASPTKTRRILFLLPYVADLSVGDFS
                     KGLNPVMQDKQIDVMMTTLDFLQNKTAKELINEFDGLIYYALDTEQHLDLLFELSLKE
                     FPVVILDKKIYELPFPTVLSDNFQGGALAASYLIEQGHSKIAYLFGTQTHPQSVRQRY
                     LGYLEALDKAGLTFHTSLDDKSATLDELLSYITKYEVTAFVCENDVVAIQTMNLLRQH
                     GYHIPEDFSIIGFDDIQAAALVDPPLTTIAQDFEQLGKLAGDALVTWLETKDRPSDSK
                     FPVSFIKRQSTKEK"
     gene            11015..12304
                     /locus_tag="IV334_001199"
     CDS             11015..12304
                     /locus_tag="IV334_001199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 125 protein"
                     /protein_id="HAP8943281.1"
                     /translation="MVYKDIPKSVQKFMEDITQKCGDIHADWAKNFTAAFANTLLTTV
                     KKQNDGTTFLLTGDIPAMWLRDSTAQVRPYLVIAKEDEELADMISGLVKKQFFYINID
                     PYANAFNEEANGAGHQTDHTKMNDWIWERKYEIDSLCYPIQLAYLLYKNTGKTDQFNQ
                     SFIEGIKKILDVFKTEQDHEHSPYTFVRDTTRKEDTLINDGRGAKTAFTGMTWSGFRP
                     SDDACRYGYLVPSNMFAVVVLGYVEEIFQTILDDKEIVQQTQKLKKEIQEGIQNHGRT
                     VNQKGQTIYAYEVDGMGNATVMDDSNVPNLIAAPYLGYCSEQEEDYLQTRRTLLSKEN
                     PYFYEGKYAKGIGSSHTPEKYVWPIALAMEGMTTSDKSEKERILNLLVATDAGTHLMH
                     EGFDVNNPENYTREWFSWANMMFCELVMDYFDIRVEK"
     gene            12317..15019
                     /locus_tag="IV334_001200"
     CDS             12317..15019
                     /locus_tag="IV334_001200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HAP8943282.1"
                     /translation="MKKKVYIISHSHWDREWYMAYEQHHMRLIELIDDLLELFEVDPS
                     FNSFHLDGQTIILDDYLQVRPEKRQAIQQAINEGKLRIGPFYILQDDFLISSESNVRN
                     MLIGMEESRKWGTPVMLGYFPDTFGNMGQTPQLMKQAGISAAAFGRGVKPIGFDNQVL
                     EAENYSSQYSEMWWKGPDQTAIFGLLFANWYSNGNEIPVEKEAALAFWKQKLADAEQY
                     ASTNHLLMMNGVDHQPVQKDISKAIHLANELFPDYEFIHSNFTDYLEAVQKDVPEDLG
                     SVEGELTSQETDGWYTLANTASARVYLKQWNTKVQRQLENITEPLATMAYEVSGNYPH
                     DQLDYAWKTLMQNHPHDSICGCSVDSVHREMIPRFEKADEVGKYLAQDSLEQLTAAID
                     TTGFPKDSFPFVIVNTAGMDKTGEAEITIELERKRFAEGIPEQLYQELENLPKRKYHV
                     ETKSGATIPAILSEETVQFGYDLPKDRFRVPYMARMIKVTLPLENMPAFSWETFALVE
                     GEAKAEEKETMIHQSGRIIENGPLHLTIEKNGTITMEDRKNKRKLNNLHIFEDIGDIG
                     NEYIFKQPFCDQPILSSNKENSEVKVLVDTPEIAKISILQEMEIPVSADERLEKEQQM
                     VVEFRYRKAERSKEKRILQIKTIMTIRKDSKKIDFETTLDNQMKDHRLRVLFPTKLHV
                     EHHEADSIFEVVKRPNHVSKSWENPTNPQHQQAFVNIHDEEYGVTVGNFGLNEYEVTE
                     DGQIAVTLLRSVGELGDWGYFPTPEAQCLGEHRFNYSIELHGPEEKFSTYLHAYAAQI
                     PFSTQQIKHHEGTLISKQQYLTIKSETFAITALKRSKFSDKVVVRGFNMSSHLEKLEI
                     TKDNGKTVILNLLEEPTKQAVVPIIQPYEIRTIGFEEENECMAE"
     gene            15004..15861
                     /locus_tag="IV334_001201"
     CDS             15004..15861
                     /locus_tag="IV334_001201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HAP8943283.1"
                     /translation="MYGGIEAGGTKFVCAVSDGGEILEKLSIPTTTPEETLDQVFEFF
                     DRYELKGIGIGSFGPIGIDKTKDNYGYVLATPKKGWAQFDFLGTIKERYNIPAAWTTD
                     VNAAAYGELKQGAAQGKNSCIYLTVGTGIGAGVVINEEIFSGIAHPEMGHIWVKRHPD
                     DHYEGTCPYHKDCLEGLAAGPSIEARTGIKGQDLPQDHPVWAIQAYYIAQALINYTLT
                     LAPEKIILGGGVMNQDHLLQKIRQQFVELMGGYMETPQVSEYIVRWGLPNESGIIGSL
                     LLAEHEHQK"
     gene            complement(15932..16789)
                     /locus_tag="IV334_001202"
     CDS             complement(15932..16789)
                     /locus_tag="IV334_001202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HAP8943284.1"
                     /translation="MKITITLPMNHPETTIRDLLEKEWLVPRKVRHFLRIRKNVWINQ
                     QPALFHHEVKGNDQITLLFEETDYNYQEVQLGDAKHIRVLYEDEHLIIVDKPAGMKTH
                     PNEPTENSTLLNHLAAYLDTKQQRPYVVHRLDKETSGAVLFAKNPFVLPILGRMLEQK
                     MIYRRYQAIVWGEIAKDMTITNKIGRDRHDRRKRVVDDKNGQSALTHVTVNKVLSEQT
                     QVYCVLETGRTHQIRVHLAHKGHPIVGDPLYQNRTAERLMLHALELHMIHPFTQEKIV
                     GKALPGLWE"
     gene            17000..19171
                     /locus_tag="IV334_001203"
     CDS             17000..19171
                     /locus_tag="IV334_001203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HAP8943285.1"
                     /translation="MDFQSILEKIKAALISFWRWIRPYLGQFHRWRKRIWKKYHVNKL
                     ILLIGLICVLVTSIYLFILAKQANVETLKSGLSQSTVIYDKKNEEAGTLYAQKGTYVE
                     LNAISPYIQDAVISTEDRNFYEHHGFDIKGIARAAVRMVLSGGTSGGGGSTITQQLAK
                     NAYLTLDQTFDRKAKELFLAIEIEKKYSKEEILTMYLNNAYFGNGVWGVQDASRKYFG
                     VDATNVTLSEAATLAGMLKGPGIYNPIDHPKNANDRKNTVLSVMEENGKITKEQEQAE
                     SQTDISAYLNDTYDNSDSGYRYPYYFDAVIDEAVNEYGLDEEDVMNKGYKIYTALDQD
                     YQKQLEATYQNSALFPANASDGAMVQSASVALDPNTGGVQALVGRRGDHVFRGFSFAT
                     QMRRSPGSTIKPLSVYTPALEAGYKPSSILKDEPQSYYDAHNFDGTYQGEVPMYQAVA
                     QSLNLPAVWLLHEIGLQKGYDKAEEFGLPLAKSDKYYGLALGGLEKGVSPLIMAGAYS
                     AFANDGQMYTPHLITKIVDSTGAVIVDNTNKKPKQVISKETADEMTSMLLGTFSNGTA
                     AAANPAGYTIAGKTGTTETNFDATKANDQWMIGYTPDVVISTWMGYEESSKLHYLEGT
                     SGNVVGKVFKSAAEGILPYTSKTGFSVADAYATGGQVVAADQVGNTGNSNGESGNWQD
                     NLNQYGEQAKEGLKNFGDMVKEGVQGIGEAAKDLWRKYQGE"
     gene            19239..19580
                     /locus_tag="IV334_001204"
     CDS             19239..19580
                     /locus_tag="IV334_001204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HAP8943286.1"
                     /translation="MSNIYDSANQIEREIRELPEFKALKEAYEKVQANEEAHKLFKDF
                     QAMQIELQEKQMSGQEFSDEDAMKAQEMAVKIQSEEVINDLMQKEQGFSTIINDLNRI
                     IMTPVRDLYSE"
     gene            complement(19856..21610)
                     /locus_tag="IV334_001205"
     CDS             complement(19856..21610)
                     /locus_tag="IV334_001205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP8943287.1"
                     /translation="MEERIQKSYQKYHANAFQTLLHLYQGNYWRFAMSGLFFIIKHLP
                     SWLMPIAIANVINAASSKDSSQLQAIYFNAFLMACLIGQNILTNYFHVKYHSTSIRQV
                     EAGLRATLIKKIQELSIPYQKELQSGRIQSKIIRDVEAIQTLSSQVFVSGLNIAVNLF
                     VALGITLYKSPVVFMFFLLTVPLAAFVTLFFRKRIRQSNSDFRQEMEETSAKVIEVIE
                     LAPIARAHGLEKHETTRLNKHFTQIYEKGFHLDILQSVFGSVSWASFQIFQLICLIFT
                     GILALQGRIQPGDVVMYQTYFTTIVNSVSGMITLIPTISKGMESITSIGELLIAGDVE
                     TYHGKKKIQHLDGNFRFEDVSFLYPDSLKPIIDHFSLEVKAGETIAFVGPSGSGKSTL
                     LNLLIGFIQPTSGKIFLDDQPLDELDMRSVRNYLAVVPQTTLLFSASIKENITYGLKN
                     VSKERLDEVIEAAQLSSLIDQLPDGLDTLVGEHGNKLSGGQKQCISIARALIRQPKII
                     LLDEATSALDNQSEKKIQQALNYLTETPTTFIVAHRLSTIKEADKIVVIDEGKIVEIG
                     TYEKLLAQKGYFYRLKNE"
     gene            complement(21621..22457)
                     /locus_tag="IV334_001206"
     CDS             complement(21621..22457)
                     /locus_tag="IV334_001206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HAP8943288.1"
                     /translation="MFPLNLGIRAHDLDVRNREEIVQKVSQLHLSHIQFAPQKAFPEQ
                     LKLTDMTLGSAAYFGEYFQKNNIELSILGCYINLSSKNQEVRAQALATFKQQIALCSA
                     YQAKMIATETGSVSVGYTKENFTEEAYQIARNSIIELVAHAENFGVTVAVEAGINHPI
                     YNYQLAKRLIDEVASPNLKIILDCANLMHKENHADQEKVIRGALDNLHGYITALHLKD
                     YIMENGKIRMVPVGKGCLDFRPVLHYIKYERPLMYATLEATPEIYVPEAIEHLQFIYQ
                     DC"
     gene            complement(22459..23142)
                     /locus_tag="IV334_001207"
     CDS             complement(22459..23142)
                     /locus_tag="IV334_001207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnogalacturonan acetylesterase"
                     /protein_id="HAP8943289.1"
                     /translation="MPTIHIAGDSTAAEKDENKRPETGWGEKISAYFSPEIQINNQAK
                     NGRSTKSFIQEGRLARLASQFQPNDFLFVQFGHNDQKIQEERGTLPFGSYLKNLQIFF
                     ECAKKANVQMVLLTSVTRRDYLENGTLNPDILGDYPKAMRAFAEKHHIPLLDVFSRSQ
                     ELFQTFSKEETKKFYLHLMPDTCKNYPEGLKDNTHFSPEGADKVARIIVELIKESRLP
                     LANYLQKGV"
BASE COUNT         7633 a         4149 c         4777 g         6650 t
ORIGIN      
        1 atttgcaatc attatgagcg agttgagaaa taaacgactc gttaaagttt atcaaacgat
       61 gtccttacta ccttattttc tatcttgggt cattatcagt tactttgttt acgctttttt
      121 aagtcctgac aaaggaatat tcaatcagtg gatcactgga catggaggag aagctgtcaa
      181 ttggtataat gaaccgaaat attggccgtt tatcttggtg ttcattggta catggaaagg
      241 gattggttat aacagcatta tttactttgc atctgttatg ggaatcgatc cgacttatta
      301 tgaagctgct atggttgatg gagctagcaa atggcagcag atcaaacatg tgacgatacc
      361 acaacttatt ccactaatga cgattttaac gattttagcc gtaggaaaca ttttccgagc
      421 tgattttggc ctgttttata acattcctag gaattccggt gcattatatg atgtgactca
      481 agttttggat acgtatatct acaatggcct gacatccaca ggagattttg gtatgacggc
      541 tgctgcagga ctttaccaat cagttgtcgg ctttgtcttg ttgatgatca caaatacgat
      601 cgcccgacgt tttgatagcg attctgcatt attctaagga ggaaaaaaat gaaaaaaaag
      661 aaagtggaga gagtccaagt ccgctctttc aataagacgg ctaatttttt ctctaatata
      721 atgattgcac tctttgctat ttcctgtatt ttgccattta tatttgttat cgccatctct
      781 tttaccgatg agtccatatt gtcgactcac gggtacagtt tttggccaga aaagttcagt
      841 atatttggtt atacgtactt gttcgaccag atgcaggata aaattttcca agcgcttttc
      901 gtcactgtac ttgttactgt tttaggtaca ctgatcaata gcacggctac ttctttatat
      961 gcttatgcga tttcacggtc aaactttcca ttccgtcgtt tttttaccgt tttttgtttg
     1021 attacgatgt tgttctcacc agggatggtg gccaattatc ttgttatgac aaatcttctt
     1081 cagttaaaag atacgatttg ggcattgatc ttgccaatgg ctgtcagtcc cttcaatatt
     1141 attgtgatgc gcacattctt taaacgttcg gtctctgatt cgattattga atcggcacgg
     1201 atcgatggag ctagcgaact gcgaatattt gttcagatcg ttttaccgtt agctgttcca
     1261 ggtattgcga caatcagttt atttgccgca ttgggttact ggaatgactg gttcaatgct
     1321 ttactataca tccaagatga taagctagtt ccactacagt atcttttgat gaaaatccaa
     1381 agtaatattc aatatctgac acaaaattca ggagcaggaa gccaattgac aggcggatta
     1441 gcctcagttc caggagaatc tgccagaatg gcgatcgtag tcatctcgac gttgcctatt
     1501 gcgatcagct atccattttt ccagaagcat tttgtcaaag gtctaacaat cggcggagta
     1561 aaagaataaa gaaacaaaaa tataaatcat aaaattagga ggaagaaaaa tgaagttatg
     1621 gaagaaacta gcctttacag gtgtttcagc gttgttgttg ggaacattag cagcatgcgg
     1681 aggttctggt tctaaagacc aggcagaagc tagcaattcg gacactttgc agatgtatca
     1741 aatcggagat aaaccagata atttcgatca attgatggaa gtagcaaaca aacgaatcga
     1801 aaaagagatc ggtgtaaaag tcaatatcaa ttacattggc tggggcgatt acgaaaagaa
     1861 aatgaacgtc atcatttctt ccggggaaaa ttatgatatc gcctttgcga acaactatgt
     1921 ttccaatgcc caaaaaggag cctttgctga tttgactgaa ttagcgccta agtacgctga
     1981 aaaagcttat aatgatttag atgaagcata tattaaagga aatctggtca atggaaaatt
     2041 atatgctttc ccagtcaatg gaaatgtctt tgctcaacaa gtacttactt ttaacaaagc
     2101 tttactagat aaatacgatc tttcaattga tggaatccaa tcttacgctg atgcagaaaa
     2161 agtcttgaag gaattccatg aaaaagaacc aaacacggca gcttttgcca ttggacaagg
     2221 cttcaaagtc caagggaact ttgattatcc tttagggaat acgttaccat ttgctattga
     2281 tctggatgga gatgattcga aaatcatcaa ccaatacgat aatgaaacgt atatcggact
     2341 gttacgtaca atgcatagct ggtacaaaca aggactcatt ccaagtgacg ctgcaacgag
     2401 caataaggat tatccactag aaggaaatac ttggctaatg cgtggagaaa cacaaggacc
     2461 gtatgattat ggcgatacaa ttttgacaaa tgcagcaaaa caagaacttg tctcaaaagc
     2521 aattactgtt ccactaaaat ctacagctca agcacaaatg gcaaattttg tcgtatcaaa
     2581 tacttcaaaa aataaagaaa aatcagtaga acttctaggg ctgcttaaca gtgatccaga
     2641 attattgaat gggcttgttt gggggatcga gggagaagca tgggaaaaag tcgatggttc
     2701 agatgataaa atcaaattgc tagatggtta tcagccaaat acacatatgt ctgcttggaa
     2761 tacaggaaac aacaagattc tatatacaca agagtctatt acggatgaca tgattgccaa
     2821 acgtgatcaa tcgattgctg atgcagaaac ttcaccgatt ttaggattca gcttcaacac
     2881 tgacagtgtc aaaacagaat taagtaatat ttctaatgtc atgaatcaat atttagatgg
     2941 attgaataca gggactgttg atccagatga aacattaccg aaattgaaag acgctttgga
     3001 caaggctggc tatgataagg tattgaaaga aatgcaaaaa caatatgatg agttccgaca
     3061 agaataataa tgaataaaga caaaggggtt gtgacaaaag acgtgtcaca acctcttgtt
     3121 ccgaataaac ggtgttcaaa agccagtttc tcggtattaa gctaaatata tgtaaaaatg
     3181 tggaaagcca tattcacaaa atttttctta ctactcgaaa ctaagcggct atttcacaaa
     3241 ctcttggtaa acggtgttca atcagctaac cctcggtatt cgctcacaaa acgacaaaat
     3301 atgaggaact atttttccgt ttgtttctct aataatcagg tcaaggtgct gattgcccaa
     3361 cctttttttg tataatgaga aagtataaac ttaatgtttg gaggagaaga tatgttaggg
     3421 aaggttttgg aagagttatt tatccggatc tgggtcatta taaaactaac cttgtatttc
     3481 tggatatata catttgccgg cgggatcatc tttggattag gtgcggcatg gaaaacagtc
     3541 aatgaactat tttatttgta cggattcgag tataaagaaa tcacaataaa aagaggctgg
     3601 aatatatata aaagaaattt tttaagaggc aatctgctat ttagtttatt tttatctggt
     3661 actgctttat tgagttacaa tttatattta tctgttcaaa tcaaagggct cattttttta
     3721 gctattgatt ttattttact ttttgccttg ctctgcctat ttgctactta tcaatacagt
     3781 atgattttag atagtgaata tacgatctct gttcctaatc tgctaaagct aagttttatt
     3841 tcggtttttt ccagcttttc gtcttttcta aaaataatta ttggtagcgg tataatttta
     3901 ggcgtgactt ggcaattcaa aggattgatc ctgtttggtg tcattggtct tttgacggtt
     3961 tggaatggta cgatgacaac acattggcgt gaagaattag ataaacagtt ggaaagttat
     4021 gaataagttt cggattctat tttcaaaagg aaccttacta aatcgtctgt tgcggaaata
     4081 cgcggttatc atgatcggtg taacgatgac tgctaccgtt atttttagta tccatacttg
     4141 ggaacaaaat caaaaacagg cagaaaacat gacaagtgat gccgttcaaa gcacaagcag
     4201 aatgctgaat gataaaacaa ccttgagtag gatcatcaaa aaccagcttg tagggaattc
     4261 cgaaaagatc gaaaatgtga cgacctactt aacaaaaccc atagatcagt atctgatgta
     4321 tgtatacgaa cagcaaaaca gtaccgatga attagtttct ttcccaaatc aaatcaaaga
     4381 tctctacgca aattatgaag aactaagtgc gatatatatc gtgttgaatc aactaccgga
     4441 gtattatgaa tcttctagag aaaacaaagg tggggaaata aaaaagggga caccaaaatt
     4501 actagacgcc ttttatatca agttgcctat cacacaagga ggagctgagt caggaagtat
     4561 ttttattggt tttcaaaaga aagaactaga tatgatttta gaaaatctaa cgtcattcaa
     4621 tggactctct ttatacatga tttctggaac gacaaatcga ctatacacat tccatgataa
     4681 taaaatagct aaagaaacgt ttgaaagaca agaaatgatc atcacagaga gtctgaagaa
     4741 aaattcacaa ttgccgattg acgatctgga aaaaaacaat tttgttcaac accaagaact
     4801 gtctgatgat tatcgtatat tagctgtact agataaaaaa agtgtaaaaa tcgaaacggg
     4861 aaaaaatctt gccccgctga tcattggtat cattatttta gacaatatac tgcttatctt
     4921 tctttataaa acattcaaac gatattcaca gcaagtatct atcgtcatgc aggccatgga
     4981 tcaaacagca gcagggaatt tagagacgcg gatcgacata acaaacacgg aatatgaact
     5041 gagggaactt tcaatcggta tcaacgaaat gttggatagc atcaatcagt ttgtagaaga
     5101 catctacaaa ttagaaatca aacagcaaga cgcacatatg cgggcgcttc aagcacagat
     5161 caatccacac tttttatata atacattaga atatatccga atgtatgcaa tcagcgaagg
     5221 aagtgaagaa ttagcagatg ttgtgtatgc gttttccgct ttgcttcgaa acaatacgaa
     5281 tcaagagaaa acgatcactt taaaagaaga actggacttt tgcgaaaaat atgtctattt
     5341 gtatcaaatg cgttatccta atcgagtagc ctatcatttt atgatcgatc ctgatttgga
     5401 aaagatcgaa gtacctaaat ttgtgatcca gccattagtt gaaaattatt ttaaacacgg
     5461 tattgatttt actcgattcg ataatgcgct aagtgtcaaa gtcttgcagg aaggcaaacg
     5521 ggtaaggatc atcatcaaag ataatggaaa agggatgact gaaaaacggc taaaacagat
     5581 tgaagaaaaa ctgtctcatc ctaaagtgga acttcacggt tctatcggtc tgcaaaacgt
     5641 gaatgaacgt ttacgtgcca gcttcggcag cagttattat atgtcgcttg agaataatga
     5701 gacaggtgga ctgaccgttt ctattacatt caaggaggga tgagcgtgta tcaagtgtta
     5761 ttggtagatg atgaatacat gattttgaat gggttaaaaa agataattga ttggcaaagt
     5821 ttaggctttc aaatagtagc aacagcagag aatgccaaag aaggactagc tgtacttgaa
     5881 cagcgacaga ttgatttagt agtaacggat gtgacaatgc cagaaataaa tggactggaa
     5941 tttattgaag cggctcaaaa agaacgtcat aattttgaat ttatgatttt atcaggatac
     6001 caagaattcg attatttgaa aggaggtatg caattaggcg ccgtgaatta tttgatgaaa
     6061 cccgtgaata agttcgaatt ggttgaaagc ctcaagaaaa tcaaaacacg gttagatcag
     6121 caaaatgaac aaaaaaatca acaagaaatc tatcaagaac tgttattcag tcaatggttg
     6181 aatgaagaat tggatgaaat aagtgaagaa gaaataatag gacaaataag tgagaagcag
     6241 cataggatag tattgattca actggcaaga actcatgata ctcttatcaa tagatggttg
     6301 caagaacggc aacaaatctt ttactatcaa cgaaattacg gagaccgtgt attgatcacg
     6361 ttgttgttgg aggcagatga agttgatggc ttctgttgtt ttgttcaaga aaactttcct
     6421 gatcaggaat ggctgatcag tatcggggaa gagacagatt ctttggacaa aatccctgaa
     6481 acattccaac aagcgaaaga caatctacag ttgcatcaat tttatggaga taaagacgag
     6541 catgtttttt acgcagtaca agcagaaaca aaagatcaaa ccatcgattt ttcaagtttc
     6601 agacgcgtgc tgcaaaataa acaattggaa gtcgctcaaa aaatgatcac tgattttttt
     6661 gaacagtttc agttagcagc catgatgccg gaagatatac gatattccag ttttttactg
     6721 tttatggaga ttcaacggga attgattgat ctggaagatg aggaatattt acaaggaatc
     6781 gaaaaaatcc atcaggcaaa aacggtgcaa gaacttcagc agctacttct gtcattcatc
     6841 aaaaagcatc agagacagaa aaagtacagt gacaatgtgg aaaaagtaat cgtaatcctg
     6901 caccagcatt atcaagaacc acttacactc aaagaagtgt cagaaaacct gcatttaaat
     6961 gtcatgtatc taggacagct gttcaaaaaa gaaacgaaaa aaagtttttc agcttatttg
     7021 aaccatttac gtatggaaaa agcgaaacaa ctgcttttgc acagtaatca aaacatcaat
     7081 gagatagcaa gtgagattgg ctacaacaac accacgtatt tctctaaatt attcaagaaa
     7141 atcgtcggca gatcccctaa agagtaccga gaaaacagtg gagaatcata aaagaaaaag
     7201 ccaatgtaat gaaataactt ataaaaaatt gtctagaaat ctaattttgg gctgttctta
     7261 ttacttgagg caaggcagct ttttcatgac ctctcgataa acggagttca aaagccagtt
     7321 tctcggtatt aagccaaatt tatgcaaaaa tgtggaaagc cattttcaca aaatttttct
     7381 taatactcga aactaaacag ctttttcacg acctctccac taaacggtgt tcaatcagct
     7441 gaccctcggt attagctcaa aaaatggcaa aatatgagga actattttgc catttgtctc
     7501 tctaatactc aggtcagaac gctgatttca caacctctca ataaacggtg ttcaaaagct
     7561 tgcatctgcg gtaataagac caaccaaacg aaaaatatga ggatctattt ttgcttggct
     7621 gttcttatta cttgatgcag gacagctttt tcacaacctc ttgttaatta tagtttgata
     7681 aggaaaaagg caaatctttt gcttgatatt tcttagggtg agggatgatc cctaattggt
     7741 aagagatcgt gccgcctttc agcaaatctt gatgggtaaa atataatttc gtatattctc
     7801 tgtccatccg tttgatttta tggataaatt gttgctgcgg ccgattaggc gttgcttcaa
     7861 tcgtcaattc ctttccattt gacaaaagca aaacagcctt gtcaattaat ggcataccga
     7921 taacatattc accggttcca ggcgtgacag gataaaatcc cagactattg aagatatacc
     7981 aagcggccat acttccattg tcctcatctc cagggtaacc cattggagaa tcgtcgaagg
     8041 tctgagtcat caactgttta agcagtggct gtgccatttc aggttgtcct atataactga
     8101 acaagtaagg atagtgaaaa cttggctggt ttgaaatagc taattgtcca aattctatcg
     8161 cggccatctc actcatttcg tggatctcaa aaccatatcc tcctgggtca aacgttgggg
     8221 gctgattgca cagctcaatg agtttttctt tatatctctc actgccacca tattttccga
     8281 tcagtcctga aaaatcgtga taaacagcaa agctgctttg ccaagcactt ccttctgcat
     8341 aatcttgtcc ccaacgatta gctgaaaatg gttctctgaa cttgccatct ttatcttttg
     8401 aacgcataaa tcctgactca tagtcaaaga tattttgata gttcaatgat tgctgctgat
     8461 atttcttcgt catttcctct tcttctagta ccttggcaac ttggctaata caaaaatcgc
     8521 tataagcata gtctaatgtg tgattgactg attcgtggta gtgattagga acatagccat
     8581 atgtcaagta atcttctgtt ccttggcgtc cataatttgg atgtttgctt tgaaccgtag
     8641 cacctttttc cattgccgtt agaaattcag gcatcaagtc tcgtcggata tcttttacag
     8701 cagcatctgc aatgaccgca tcgatcaatg tcccaggcat aagtccacgt tcatcaggag
     8761 acagccattt tggcaaatag ccactttctc tatagctgtt caaaaaacct tccagcattt
     8821 cttcgtattt ttctacagca atcaaagaat aaagaggata aaccgtttta tacgtatccc
     8881 aaaacccatt atttgtatag agaacaccag gttttataga tcgactagtc gtatcatagt
     8941 ggattttttg cccactagga tcaatttcat aaaatgtttg tggaaataaa aacacccgat
     9001 ataaattatg ataaaacgtc cgcaattgtt gttgatcatg gtgtgtgagc tgtatacgat
     9061 caaaatagga ctgccattgt tcttgactgt gagcaaggta agtctctttg ctttggtttt
     9121 gttctagttc caggttataa gctgcttgtt ccgagctgat aaaagatgtc ccaaatcgga
     9181 tagtttgttt ttttgtctgt tcaaaataca acgggatcga gtcgctattt ttgttggaaa
     9241 cagctaactc atcaattgtt aaaggatatt caaatctcaa tataaaataa aaagaaaaat
     9301 caggatcttc tgaaccagca aaatgtgtga tttttccttc cactgtgtaa ggatctataa
     9361 caaagagctg atggcgtccc ggaaaggata aaagtaaacc gctgtcattt ttgctgtaat
     9421 caatagataa gattccgcca tacatgctag gtatcaatgt agaacagatg ccataacgta
     9481 attgacagat cgacagatga gtcgggcaaa atatactttc ttctgggcgg taagaacttt
     9541 gagcatggaa aagggtgttc tcctgtagtt ttccattgat cggcgtaaac agcatatgac
     9601 tgaaatcacc catccaagga cttggttgat gggtaagacg gtagccttga aaaacgcggt
     9661 catcaggatg aaaccaccag ctgccgtttt gatccgtagt ttgcggtgca aaataattca
     9721 tcccaaaagg aacgccagta taaggctggc agttcccatt agagaaattt ggctgattcg
     9781 ctgttgcatg tctagtatct atttgaccga tattcatttt atttctcctt tgttgattgt
     9841 cttttgatga atgaaactgg aaatttacta tcggatggtc gatcttttgt ctctaaccaa
     9901 gtgaccaaag catctcccgc aagcttacct aactgttcaa aatcttgtgc gatggttgtc
     9961 agtggcgggt caacaagagc tgctgcttgg atatcatcaa atccgataat tgaaaaatct
    10021 tcagggatat gataaccatg ctgtctcaag agattcatgg tctggatagc gacaacatcg
    10081 ttttcacaaa caaaagcagt tacttcatac ttagtgatat aagaaagcaa ttcatctaat
    10141 gtcgcgcttt tgtcatccag tgaagtgtgg aaagtcaaac cagctttgtc caatgcttca
    10201 aggtaaccaa ggtaacgctg tcgaacggat tgtggatgag tctgcgtacc aaacaaataa
    10261 gcgatttttg aatgtccctg ttcgatcaaa taagaggcag caagcgcacc gccttggaaa
    10321 ttatcggaaa gaacggtagg aaaaggtaac tcatagattt ttttatctaa tatgacgaca
    10381 gggaattctt tcaatgaaag ctcaaaaagt aagtcaagat gttgttctgt atcaagagca
    10441 taataaatca atccgtcaaa ttcattgatt agctcttttg ctgttttatt ttgtagaaag
    10501 tcaagcgtcg tcatcatgac atcaatctgt ttatcttgca tcacagggtt caatcccttg
    10561 ctaaaatcac caacagacaa atcagcgaca tatggaagaa gaaacagaat ccgtcgtgtt
    10621 tttgtaggag aagcaagaat ctgttccgga gaagcggatt taacgaaact gcctttcccg
    10681 cgaacgcgat aaataagtcc taattgctct aattcagtca atgcacgttt ggaagtgatt
    10741 ctgcttactt tataggtacg tgaaagttct ttttcagttg gtagttggct gtctacaggt
    10801 aattcaccag ttaaaatagc cgtttgcaaa tcattaaata ttgtctggta caatggttga
    10861 gtcatataaa cacctcttaa tgatatatca tattcaaata ataccataat ggatgaaaaa
    10921 gtaaataaaa agatattttt tgaaagcgta gacataattg gctgtatgaa gtataatctt
    10981 taaagatata tcaaatagaa agaaggtttt attagtggtt tataaagata taccaaaatc
    11041 ggtgcagaaa tttatggaag atatcacaca aaaatgtggg gatatccatg cggactgggc
    11101 aaagaatttt acagcagcat ttgccaatac tctgttgaca acagtcaaaa agcaaaatga
    11161 cggaaccacc tttttattga cgggagacat tccagcgatg tggttaagag actctactgc
    11221 acaagttcgt ccttatttag tgattgcaaa agaagatgag gaattagctg acatgatcag
    11281 cgggttagtc aaaaagcaat ttttttatat caatatcgat ccatatgcga atgccttcaa
    11341 cgaagaagct aacggtgctg gccaccaaac agatcataca aaaatgaacg attggatctg
    11401 ggaaagaaaa tatgaaattg attctttatg ctacccaata cagttggcct atcttcttta
    11461 caaaaataca ggaaaaactg atcaattcaa tcaatctttt attgaaggta tcaaaaaaat
    11521 cttggacgtg ttcaaaacag agcaagatca tgaacactct ccttatacat ttgtacgaga
    11581 cacgacaaga aaagaagata cgctgatcaa tgacgggcga ggtgcaaaaa cagcttttac
    11641 aggaatgaca tggtctggtt tccgtcctag cgatgatgct tgccgttatg gatatctggt
    11701 accatccaat atgtttgcgg tggtcgtgtt aggttatgtg gaagaaattt ttcaaacgat
    11761 actcgatgat aaagaaattg tacagcaaac acagaaattg aagaaagaga tccaagaggg
    11821 gattcagaat cacggacgaa cggttaatca aaaagggcaa acgatttatg catatgaagt
    11881 agatggaatg ggaaacgcca cagtcatgga tgacagtaat gtgcccaatc tgatcgccgc
    11941 accttacttg ggatattgtt ctgaacaaga ggaagattat ctgcaaacaa gaagaacatt
    12001 gttgagtaaa gaaaatccat atttctatga aggaaagtac gcaaaaggaa ttggatcctc
    12061 ccatacgcca gaaaaatacg tttggccgat tgcattagct atggaaggaa tgacaacgtc
    12121 agacaaatct gaaaaagagc ggatattgaa tttacttgta gcaactgacg caggaacaca
    12181 ccttatgcac gaaggattcg atgtgaacaa tcctgaaaat tatacgagag aatggttttc
    12241 ttgggcaaat atgatgtttt gcgaattagt catggattac tttgatatta gagtagaaaa
    12301 gtaggaggta tcgtaaatga aaaagaaagt atatatcatt tctcatagcc actgggatcg
    12361 tgaatggtac atggcttacg aacagcatca catgcgactg attgaattga tagacgatct
    12421 actagagcta tttgaagttg atccgtcctt caacagtttt catttggatg ggcaaacgat
    12481 tattttagat gactatcttc aagtaaggcc agaaaaaaga caggcaatcc aacaagcaat
    12541 caatgaagga aaattgagaa ttggaccatt ttatatcttg caagatgatt ttcttatcag
    12601 ctcggaatcg aacgtgagaa atatgctgat tggcatggaa gaaagccgta aatggggaac
    12661 ccctgtcatg ttaggctatt ttcccgatac atttggaaat atgggacaga cgcctcaatt
    12721 aatgaaacaa gctggtattt cagcagcagc gtttggccgt ggtgtaaaac caattggctt
    12781 tgataatcaa gttttggaag cagaaaacta ttcttcccaa tactcagaga tgtggtggaa
    12841 aggccctgat caaacagcaa tctttggtct gctatttgcc aattggtata gcaacgggaa
    12901 tgagattcca gtagaaaaag aagcagctct tgccttttgg aagcaaaaat tagcggatgc
    12961 agaacaatat gcctctacga accacttatt gatgatgaat ggtgttgatc atcaaccggt
    13021 acaaaaagat atatcaaaag caatccatct cgcaaatgaa ctgtttccag attacgagtt
    13081 tattcattct aattttactg actatctgga agcagttcaa aaagatgtgc cagaagactt
    13141 aggctcagta gaaggagaat tgacaagtca agaaacggat ggctggtata ctttagccaa
    13201 tacagcatct gctcgggttt acttaaaaca atggaatacc aaggtccagc gacagctaga
    13261 aaatattaca gaaccacttg cgacgatggc ttatgaagtt agtggaaact atccccatga
    13321 tcaattagat tatgcttgga agacattaat gcaaaatcat cctcacgaca gtatttgcgg
    13381 ttgttcagtc gattctgtcc atcgggaaat gatacctcgt tttgaaaaag cagatgaagt
    13441 tggaaagtat cttgcacaag attccttaga acagctgacc gcagcaatcg atacgaccgg
    13501 attcccaaaa gacagctttc catttgtcat tgtaaatact gcaggaatgg ataaaactgg
    13561 tgaagcagaa ataaccatcg aattagaaag aaaaagattc gctgaaggaa tacctgaaca
    13621 actatatcaa gagttagaaa atttgcctaa acgtaagtat catgtagaaa caaaaagtgg
    13681 ggccactata ccagctattt tatctgaaga aactgtccaa tttggttatg atttgccgaa
    13741 agaccgtttc cgtgtgccat atatggcaag aatgatcaaa gtgactttac cattagaaaa
    13801 catgccagca ttttcttggg aaacattcgc gcttgtagaa ggggaagcga aagcagaaga
    13861 aaaggaaaca atgattcatc agagtggacg aatcatcgaa aacggccctc tacatttgac
    13921 gattgaaaaa aatggtacca ttacaatgga agatcgcaaa aacaaacgaa agttgaataa
    13981 tctgcatatt tttgaagata tcggcgatat aggaaatgag tatatcttta aacaaccttt
    14041 ctgtgatcag ccaatcctat ccagtaacaa agaaaactct gaagtaaaag tattagtaga
    14101 tacaccggaa atagcaaaaa taagtattct acaggaaatg gaaatccctg tttctgcaga
    14161 cgagcgttta gaaaaagaac agcagatggt tgtggaattt cgttatcgaa aagcagaacg
    14221 atccaaagaa aaacgaatcc ttcaaataaa aacaatcatg acaatcagaa aagatagtaa
    14281 aaaaatcgac tttgaaacga ctctcgacaa tcagatgaaa gatcatcgct tacgtgtctt
    14341 gtttcctaca aagttacatg tggaacatca cgaagcagat agtatttttg aagtggtgaa
    14401 gcgcccaaac catgtgtcta aaagttggga aaaccccacg aatccgcaac atcagcaagc
    14461 atttgtcaat attcatgatg aagagtacgg tgtaacagtc ggaaactttg gtttgaatga
    14521 atatgaagta acagaagacg gacaaatcgc agtgacgcta cttcgcagtg tgggagaact
    14581 aggcgattgg ggatattttc caacaccaga agcacagtgt ctaggtgagc atcggttcaa
    14641 ttacagcatt gaactacatg gaccagaaga aaaattctct acttatttgc atgcatacgc
    14701 agcacagatt cctttttcca ctcaacaaat caaacatcat gaaggaacat tgatttcaaa
    14761 acaacaatat ttaacgataa aaagtgaaac gtttgcaatt acagctttga aacgcagcaa
    14821 gtttagtgat aaagtagtag taagaggttt caatatgtct agtcatttag aaaaattgga
    14881 aataactaaa gacaatggaa aaactgtcat attgaatctg cttgaagaac caacgaagca
    14941 agcggttgtc ccaataatcc aaccttatga gattcggaca atcggattcg aggaggaaaa
    15001 cgaatgtatg gcggaataga agcaggcgga acaaaatttg tgtgtgcggt ttcagatggt
    15061 ggagaaattt tagaaaaatt aagtatccct accacaacac cagaagaaac attggatcag
    15121 gtatttgaat tttttgatcg ttatgaattg aagggaatcg ggattggctc attcggcccg
    15181 attggtatcg acaaaacaaa agataattac ggatacgttt tggcaactcc caaaaaaggg
    15241 tgggcacaat tcgattttct tggcacaatc aaggaacgct acaatattcc tgctgcttgg
    15301 acaacggatg tcaatgctgc tgcatatggg gaattgaagc aaggagcagc acaaggaaaa
    15361 aatagttgta tttaccttac cgtgggaaca ggaatcggtg caggtgtcgt gatcaatgaa
    15421 gaaatttttt cagggatcgc acatcctgaa atgggacata tttgggtgaa acgccatcca
    15481 gatgaccatt atgaaggtac ttgtccttat cataaagatt gtttagaagg attagcagca
    15541 ggaccgtcta ttgaggcacg cacagggatc aaagggcaag atctgccgca agatcatcct
    15601 gtttgggcta ttcaggctta ttatattgct caagcactga tcaactatac gttgacgtta
    15661 gcacctgaaa agattatttt aggcggtggg gtaatgaatc aggatcatct ccttcaaaaa
    15721 atccgtcagc aatttgttga attgatgggt ggttatatgg aaacacctca agtaagtgaa
    15781 tatatcgttc gttggggact gccgaatgaa agtgggatta tcggtagttt gctgttagca
    15841 gaacacgaac atcaaaagta agcagacaaa ttacacaaaa agaacagagt cttatcatta
    15901 tctgctgata agactctgtt cttttttatt tctattccca taatccaggt aaagctttac
    15961 cgactatttt ttcttgagta aatggatgga tcatgtgcag ttctaatgca tggagcatca
    16021 gtcgttcagc tgttctattt tggtagagag ggtctccaac gatcggatgt cccttatgag
    16081 ctaaatgaac tctgatctgg tgtgtacgtc cggtttccaa aacacaatag acttgtgttt
    16141 gttcagaaag tactttgttg acagtgacat gtgtcaaggc gctctgacca tttttatcat
    16201 ctacgaccct ttttctccga tcatggcgat ctcgtccaat tttattggtg atggtcatat
    16261 ctttggcaat ttctccccat acgatggctt gatatctccg atagatcatt ttttgttcca
    16321 acattcttcc aagtatcggc aagacaaatg gattttttgc aaataaaacg gcaccgcttg
    16381 tttctttgtc caagcggtgg acaacgtaag gtcgttgctg tttagtgtcg agataagcag
    16441 ctaaatgatt gagtaaggta ctgttttctg tcggttcatt tggatgagtc ttcattcctg
    16501 caggcttatc cactataatc aaatgctcat cttcatataa aacacggatg tgcttcgcat
    16561 ctcctaattg aacttcttga taattataat ctgtttcttc gaataacaat gtgatctggt
    16621 cattgccctt tacttcatga tggaacaaag caggttgttg attgatccaa acgtttttac
    16681 gtatacgcaa aaagtgtcga acctttctag gaactaacca ttccttttca agcaagtcgc
    16741 gaattgttgt ttcaggatgg ttcataggta aagtgatcgt gattttcaca agtgtactcc
    16801 tttcagtgca attgcagact aattgtacca tttttaaaac atatttaaga aaaaacttac
    16861 atttttctgt tcattcttat gttactctac ttgcactaac gtaaaaaaac atattgaact
    16921 acaacttaag caggaagaaa acactctttc actatggtaa aataagacga ttgaatcaaa
    16981 tagacaggag aagaagcaaa tggattttca aagcatttta gaaaagataa aagctgcttt
    17041 gatcagtttt tggcgttgga tcagaccata tctcggacaa ttccatcgat ggcgaaagcg
    17101 catctggaaa aaatatcatg tgaacaaatt gattttgctg attggtttga tctgtgtctt
    17161 agtgacaagt atctacctct ttattttagc aaaacaagca aatgtcgaaa cgttaaaatc
    17221 aggattaagc caatcgacag tcatttatga taagaaaaac gaagaagctg gcacactgta
    17281 cgcccaaaaa ggaacatatg tagaattaaa cgcgatttct ccctatatcc aagatgcagt
    17341 aatctcgaca gaagatagga atttttacga gcatcacggg tttgatatca aagggattgc
    17401 ccgtgcagca gtccgaatgg tcttgtctgg cggaacatca gggggcggag gaagtacgat
    17461 cactcagcag cttgccaaaa atgcgtatct gacattagac caaacgtttg atcgaaaagc
    17521 gaaggaatta tttttggcaa tcgagatcga aaagaagtac agcaaagaag aaatcttgac
    17581 catgtatctt aacaatgcct attttggcaa cggggtatgg ggtgtccaag acgcttccag
    17641 aaagtatttt ggagtagatg caacgaatgt tacgctgtct gaggctgcta ctttggcagg
    17701 aatgctgaaa ggtcctggta tttataatcc gatcgatcac ccaaaaaatg cgaatgatcg
    17761 gaaaaacaca gtactaagtg taatggaaga aaacggaaaa atcacaaaag aacaagaaca
    17821 agcagaaagc cagacagata tcagcgcgta tcttaatgac acatacgata attcagattc
    17881 tggttatcgt tatccttatt attttgatgc agtgatcgat gaagcagtta acgagtatgg
    17941 tcttgatgaa gaagatgtca tgaataaagg ctataaaatc tatacagctt tagatcagga
    18001 ttatcaaaag caattagaag ctacttatca aaacagtgca ttattcccag caaatgcttc
    18061 agatggtgcc atggtccaat ctgcttcagt agctttagat ccgaatacag gtggtgtaca
    18121 agcactagta ggacgaagag gagatcatgt ctttagagga ttcagctttg ctacacagat
    18181 gcgtcgttca ccaggatcaa cgatcaaacc attaagcgta tatacaccag cactggaagc
    18241 tggatacaag ccttccagta tcttgaaaga cgaaccgcaa agttattacg atgcgcataa
    18301 ttttgacggg acataccaag gagaagtacc gatgtatcaa gctgttgcac aaagtttgaa
    18361 tttgcctgct gtatggcttt tgcatgagat cggtttacaa aaaggatatg ataaagcaga
    18421 agaattcggc ctgcctttag caaaatctga taagtattat ggcttagccc ttggtggact
    18481 ggaaaaagga gtatctccat tgatcatggc gggtgcttac agtgcatttg ccaatgatgg
    18541 tcagatgtat acgccgcatc ttatcacgaa aatcgttgat tcgactggag cggtgatcgt
    18601 cgataacaca aacaaaaaac caaaacaggt catatcaaag gaaacagccg atgaaatgac
    18661 cagcatgctt cttggtacat tttcaaatgg aacagcggct gctgcgaatc ctgcaggtta
    18721 tacgatagca gggaaaacag gaaccacaga aactaatttc gatgccacaa aagcaaatga
    18781 ccaatggatg atcggttaca cgccagacgt agtgatttcg acatggatgg gttacgaaga
    18841 atccagcaag ctgcactatt tggaagggac aagtggaaat gttgtcggga aagtcttcaa
    18901 atcagcggca gaaggcatcc ttccgtatac ttcaaaaaca ggatttagtg tagcagatgc
    18961 ctatgccaca ggaggacaag tggtagctgc agatcaagtt ggaaacacag gaaattctaa
    19021 tggagagtct ggaaattggc aagataactt gaatcaatat ggtgaacaag caaaagaagg
    19081 gctgaaaaac ttcggcgata tggtgaaaga aggcgttcag ggaattggtg aagcggctaa
    19141 agacctctgg cggaaatatc aaggagagta gcccctttga aaacgaataa ccccatgtta
    19201 caatagagag taactactaa aagaaaagag ggcataacat gtcaaacatc tacgatagcg
    19261 cgaatcaaat cgaacgtgag atccgtgaat taccagaatt caaagcgttg aaagaagcgt
    19321 acgagaaagt acaagcaaac gaagaagcac acaaactgtt caaagacttc caagcgatgc
    19381 aaatcgaatt acaagaaaaa caaatgagtg gacaagagtt cagcgacgaa gatgcaatga
    19441 aagcacaaga aatggctgtg aaaatccaat cagaagaagt aatcaatgac ttgatgcaaa
    19501 aagagcaagg cttcagtaca atcatcaatg acttgaaccg tatcatcatg acaccagttc
    19561 gcgaccttta cagcgaataa aactatttta tgaaacaaca gagggtgtga aagagacgtt
    19621 cggccttgag cgatgacaaa attgcttctt attgccgata ggctgtctct tgaacaccat
    19681 ttaccaagag gtcgtgagcc taccgtttaa ctctgagtta caaggaacaa tttcaaaaaa
    19741 cagcttctca tgtttgatga ctattgtgac ttgtgaccga ggagttggca ggagaacacc
    19801 gtttattcag aaataggagc tacgacaaaa gtcttgtcat agctcctatt ttttgtcatt
    19861 catttttcaa gcggtagaaa tagccttttt gagctaacag tttttcgtaa gtacctattt
    19921 caacgatttt tccttcatcg atcacaacga ttttatctgc ctctttgatc gttgaaagcc
    19981 gatgagccac aataaaagta gtgggtgttt ctgtcaggta attcagggct tgctggattt
    20041 ttttctctga ctggttatct aaagctgagg ttgcttcatc tagcaaaatg atttttggct
    20101 gacgtatcaa cgctcgtgca atggagatac attgcttttg gccaccagac agcttgttgc
    20161 catgttcgcc tactaaggta tccagaccat caggaagctg gtcaatcagt gaagaaagtt
    20221 gcgcagcttc gatgacttca tctaaacgtt cttttgatac gtttttcaag ccataagtga
    20281 tgttctcttt gatcgaagcg gagaacaaca aagtggtttg aggaacaacg gctaaataat
    20341 ttcgcacact tcgcatatcc agttcatcca aaggctgatc atctagaaaa atttttcctg
    20401 aagtcggctg gataaatcca atcaataagt taaggagtgt agattttcca gagccagatg
    20461 gcccaacaaa agcaattgtt tctccagctt tcacctctag agaaaaatga tcgataatcg
    20521 gtttgagtga gtccggataa agaaaagaaa catcctcaaa acgaaaattt ccatccaaat
    20581 gttggatttt tttcttaccg tgataagttt ctacatcgcc agcaatcaac agttcgccaa
    20641 tagaagtgat agattccatg ccttttgaaa tcgtaggaat cagcgtgatc attccagaaa
    20701 cggaattgac gatcgtcgtg aaatacgtct gatacatcac gacatcgccg ggttggatgc
    20761 gcccttgtaa agctaatatc ccagtgaaaa tcaaacaaat cagctgaaat atctgaaaac
    20821 tagcccagct gactgatccg aaaacagatt gcaggatatc taagtggaat cctttttcat
    20881 agatttgagt aaaatgctta tttaagcgag ttgtttcgtg tttttctaac ccatgagctc
    20941 gagcaatcgg tgctaattcg atcacttcga tcacttttgc agaagtctct tccatttctt
    21001 gcctgaagtc tgagttggac tgtctgatcc ttttacggaa aaagagagta acgaatgcgg
    21061 cgagaggaac agtcaataag aaaaacatga aaacaacagg acttttataa agcgtgatac
    21121 ctaaagctac gaataaattg acagcgatat ttaatccgct gacaaatact tgagaggaca
    21181 acgtttggat agcttctaca tcacggatga tctttgactg gattcgtcca gattgcaatt
    21241 ctttttgata aggaatagat agttcttgga ttttcttgat aagggtcgct cttagtccag
    21301 cttctacttg acggatcgaa gtgctatggt attttacatg aaaataattc gtcaatatat
    21361 tttgaccaat cagacaagcc atcaaaaaag cgttgaaata aatggcctgc aactgagaag
    21421 aatctttcga tgaagctgca ttgattacat tggcaatagc gatcggcatt aaccaagatg
    21481 gaagatgttt gataataaaa aagagtcctg acatggcaaa acgccaataa tttccctgat
    21541 aaagatgcag caatgtctga aaagcatttg catgatattt ttgataactt ttctgtattc
    21601 gttcctccat aatcttctcc ttaacaatct tggtaaataa actgcaagtg ttctattgct
    21661 tcaggaacat aaatctcagg agtagcttca agagtggcgt acattagagg acgctcatat
    21721 ttgatataat gcagtaccgg gcggaaatct aaacagccct tgccaacagg taccatgcgg
    21781 atctttccat tttccatgat atagtctttg agatgaagcg ctgtaatata accatgtaaa
    21841 ttgtccaagg cacctcggat cactttttcc tgatcagcat gattttcttt atgcatcaaa
    21901 ttcgcacaat ctaaaatgat ttttaaattt ggtgaagcta cttcatcgat taaacgttta
    21961 gctaactgat agttatagat tggatgattg atccctgctt caacagcaac agttacgcca
    22021 aaattttctg catgagcgac taattcaatg atagaatttc tggcgatctg ataagcttct
    22081 tcagtaaaat tttcttttgt gtaaccgaca gagacactac cggtttccgt agcaatcatc
    22141 ttggcttgat aggcagagca cagagcaatt tgctgtttga aagttgccaa tgcttgtgca
    22201 cgaacttctt gatttttgct ggataggttg atatagcatc ctaaaatgga aagttcgata
    22261 ttgttttttt ggaagtattc accaaaatag gcagcactcc caagtgtcat gtcagttaat
    22321 ttaagttgtt caggaaatgc tttttgtggt gcaaattgga tatgggacaa gtgaagctgt
    22381 gatacttttt gaacgatttc ttcacgattt cgaacatcta aatcgtgggc acggatgcct
    22441 aaatttaatg gaaacatctc aaactccttt ttgtaaataa ttagcaagag gcagacggga
    22501 ttcttttatc aattcaacaa ttattcgggc aactttatca gcgccttctg gactaaagtg
    22561 ggtattgtct tttagtcctt ctgggtaatt tttacaagta tcaggcataa gatgcaggta
    22621 gaattttttt gtctcttctt ttgagaaggt ctggaacaat tcttgtgatc ttgagaacac
    22681 atctaaaaga ggaatatggt gtttctcagc aaatgcacgc atcgcttttg gatagtctcc
    22741 aagtatatct ggattcaatg ttccgttttc taagtaatct cgtcgcgtca cagatgtcag
    22801 taagaccatt tggacgttcg ctttttttgc gcattcaaaa aatatctgca gattttttaa
    22861 atagctaccg aagggaagcg tacctctttc ttcttggatt ttctggtcgt tatgtccaaa
    22921 ttgtacaaat aagaaatcat tcggctgaaa ctgtgaagct agacgggcta accttccttc
    22981 ctggataaat gatttggtgc tgcgtccatt ctttgcttgg ttattgatct ggatctcggg
    23041 tgaaaaataa gcagagattt tttctcccca acctgtctcg ggacgcttgt tttcatcttt
    23101 ttctgccgca gtagaatcac cggcaatatg gatagtaggc atgttatctc ttctttcata
    23161 aaggtttata ggcaatgaag cagaacagca agagaagaag acggagcaa
//
LOCUS       DACDKM010000032        23074 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20834_2016
            SAMN12640370-rid9891663.denovo.032, whole genome shotgun sequence.
ACCESSION   DACDKM010000032 DACDKM010000000
VERSION     DACDKM010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640370
            Sequence Read Archive: SRR10040891
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23074)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23074)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742835.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742835.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:04:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,012
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 29 of 75
            Pseudo Genes (incomplete)         :: 33 of 75
            Pseudo Genes (internal stop)      :: 19 of 75
            Pseudo Genes (multiple problems)  :: 5 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23074
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640370-rid9891663.denovo.032"
                     /isolate="E20834_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            101..2542
                     /locus_tag="IV334_001208"
     CDS             101..2542
                     /locus_tag="IV334_001208"
                     /EC_number="1.1.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA reductase,
                     degradative"
                     /protein_id="HAP8943290.1"
                     /translation="MKEVVMIDAARTPIGKYRGSLSPFTAVELGTLVTKGLLDKTKLK
                     KDKIDQVIFGNVLQAGNGQNVARQIALNSGLPVDVPAMTINEVCGSGMKAVILARQLI
                     QLGEAELVIAGGTESMSQAPMLKQYQSETNEYGEPISSMVNDGLTDAFSNAHMGLTAE
                     NVATQFSVSREEQDRYALSSQLKAAHAVEAGVFSEEIIPVKISDEDVLSEDEAVRGNS
                     TLEKLGTLRTVFSEEGTVTAGNASPLNDGASVVILASKEYAENNNLPYLATIKEVAEV
                     GIDPSIMGIAPIKAIQKLTDRSGMNLSTIDLFEINEAFAASSIVVSQELQLDEEKVNI
                     YGGAIALGHPIGASGARILTTLAYGLLREQKRYGIASLCIGGGLGLAVLLEANMEQTH
                     KDVQKKKFYQLTPSERRSQLIEKNVLTQETALIFQEQTLSEELSDHMIENQVSEVEIP
                     MGIAQNFQINGKKKWIPMATEEPSVIAAASNGAKICGNICAETPQRLMRGQIVLSGKS
                     EYQAVINAVNHRKEELILCANESYPSIVKRGGGVQDISTREFMGSFHAYLSIDFLVDV
                     KDAMGANMINSILESVANKLREWFPEEEILFSILSNFATESLASACCEIPFERLGRNK
                     EIGEQIAKKIQQAGEYAKLDPYRAATHNKGIMNGIEAVVAATGNDTRAVSASIHAYAA
                     RNGLYQGLTDWQIKGDKLVGKLTVPLAVATVGGASNILPKAKASLAMLDIDSAKELAQ
                     VIAAVGLAQNLAALRALVTEGIQKGHMGLQARSLAISIGAIGEEIEQVAKKLREAEKM
                     NQQTAIQILEKIREK"
     gene            complement(2872..3576)
                     /locus_tag="IV334_001209"
     CDS             complement(2872..3576)
                     /locus_tag="IV334_001209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8943291.1"
                     /translation="MKKKQKQKLLNQFRPSLDGVRGQLFRSLEEESLRRYGLPLQVML
                     DPKNRQILTIGLAGKTNEDTRINVPIDDNFNTVLKRIRSGENGIFERFRDNLLIEIVS
                     YWNDQRLKNDEPTAQAVATPVTEETVSVPEEKTEKPKADTTENTRSTDTNAALSFADF
                     SKAVAEYPKFYTEQNEHEVVINEKNADEPRKLATISMTTENEFVIEKALERKYKVKLT
                     LIPLIEQFAATAITDR"
     gene            complement(3763..4008)
                     /locus_tag="IV334_001210"
     CDS             complement(3763..4008)
                     /locus_tag="IV334_001210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8943292.1"
                     /translation="MDPIERLNSLSEEVTQTFHSDFVFLIDAEKIQHFPARNWTHDQI
                     IEELKKRFDHSLMVKPWHEHEVIYSPELPVFALIPKK"
     gene            complement(4025..4876)
                     /locus_tag="IV334_001211"
     CDS             complement(4025..4876)
                     /locus_tag="IV334_001211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HAP8943293.1"
                     /translation="MNSLTSTYRLSNGYEIPVVGFGTWQTPDGDVAVSSVKEALAAGY
                     RHIDTAQGYKNEESVGQAIKESGIPREEIFLTTKLWNANHSYELVMSSFEESLKKLQT
                     DYLDLFLIHWPNPVAFRDNWEQANADTWRAFEELYEAGKIKAIGVSNFLPHHLDTLAK
                     TAKIMPMVNQVFLAPGELQTTVVEYAKKHDMILEAYSPLGTGKIFDVPEMKQIAEAHD
                     KTIAQVALRWSLQHEFLPLPKSVTPSRIKENTELFDFELTEEEMKQIDQLDGVVGKAK
                     DPDTTQF"
     gene            5041..6045
                     /locus_tag="IV334_001212"
     CDS             5041..6045
                     /locus_tag="IV334_001212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein"
                     /protein_id="HAP8943294.1"
                     /translation="MDIYLKKAILHIIDRETGSPVFSQKELDLTKEYIRDFLQKKIQK
                     ISSAQTKTGQLAEESMIVESLKIAQENFVEASEAIVQRWFDIYQESEEAPSADVFVVL
                     YELDTVMYLSLLKVNYREAYTHFVEADEAGIDNRLILNRAILGAKTQKADEAFAVNLT
                     DWTYELIEKKYEFSGKKELYFSGRVIESVPAPSLEENVKVIQKVAKHLGKKFETEEFD
                     IMADLKEAVYDTIEEKGRLDHEMIAEKVFKENITAKMAFKEEVQEQGFVPEAPPVKEV
                     QEISEKKFGKQKFKLSNGIELIVPVDVYRNPDLIEFVNNPDGTISVMIKNVDEVLNRL
                     "
     gene            complement(6286..6642)
                     /locus_tag="IV334_001213"
     CDS             complement(6286..6642)
                     /locus_tag="IV334_001213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HAP8943295.1"
                     /translation="MKKLIGVLVVLGIFLGGSFAAYHYFYGGEAYYTKITTNGEMSAT
                     QADNGEKFTTYTYKQAAYNKDGEKKQVTLREEREHPLKMNAYLKLKVNPRKGVLSWEE
                     IKASEVPKNAAEKIDN"
     gene            7010..8197
                     /gene="tuf"
                     /locus_tag="IV334_001214"
     CDS             7010..8197
                     /gene="tuf"
                     /locus_tag="IV334_001214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HAP8943296.1"
                     /translation="MAKEHYDRSKPHVNIGTIGHVDHGKTTLTAAITTVLGKKGLANP
                     QDYASIDAAPEERERGITINTAHVEYETEKRHYAHIDAPGHADYVKNMITGAAQMDGA
                     ILVVSATDGPMPQTREHILLSRQVGVKYLIVFLNKVDLVDDEELIDLVEMEVRELLSE
                     YGFPGDDTPVIKGSALKALQGDPDAEAAIMELMDTVDEYIPTPERDTDKPLLLPVEDV
                     FSITGRGTVASGRIDRGAVRVGDEVEIVGIKPETQKAVVTGVEMFRKTLDYGEAGDNV
                     GVLLRGIQRDDIERGQVLAKPGSITPHTKFKAEVYVLTKEEGGRHTPFFNNYRPQFYF
                     RTTDVTGTITLPEDTEMVMPGDNVTIDVDLIHPIAVENGTTFSIREGGRTVGSGIVTE
                     IEA"
     gene            complement(8385..10976)
                     /gene="mprF"
                     /locus_tag="IV334_001215"
     CDS             complement(8385..10976)
                     /gene="mprF"
                     /locus_tag="IV334_001215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysylphosphatidylglycerol
                     flippase/synthetase MprF"
                     /protein_id="HAP8943297.1"
                     /translation="MLKNTIQWLKNHLGLFKTIFLISVIVIIVGELMSIGKTLSIQQL
                     GETFATIPVWKSGLMLLIGLISVLPMVGYDIILNRMLEQKQNPRYLFETSWLINTINN
                     IAGFGGFVSVGLRSELYGQKKESRNVIQALSKVFLFLMAGLSIYSLLSFLLVVFTPVA
                     PFLKQYWIWLIGGGLYFPVVFLFTTFKKKGFLGGTSLKTQGQLLLVSLLEWTGVLFSF
                     LSVGYLMEIRIDLWQTIPLFIAASVIGIVSMIPGEIGSFDVMMIIGLSAIGVPREIVV
                     VWILLYRLFYYIIPFLIGIVFFFKNIGSTFDQRYSGIPKQLATEIAHKIVVVLLYFSG
                     IMLVLSATIPQAFTEFRWLHSLNPLKFHFIIQFPSILLGFLLIIMGRGIAARVKRAYL
                     PTIFLIVLALLYVLLSDFSFTPVVFLSILLLIILASKNELFREQLIYSWEWRTIDGII
                     IGALSLLYIVIGVYNLPDFPHRRHHFISFFLFPSEKIWFSGLLAIIAVSFMIVLFVHF
                     LQGEKKQIGEAFNEEKALKILTTYGGNSDSQLIFLKDKRMFAYEKDGEPTVLLQFACF
                     NNKCIVMGDPSGKKEDFPEAIEAFIEETDRLCYLPVFYETSEEIVMILHEFGYDFIKM
                     GEEAYVDLNSFTTSGKKMKGTRAVLNRIEREGFTFDVLQPPFSTEQMSTFKNISDNWL
                     GSRKEKGFSLGFFSEDYLQRAPIAVVKNTQEEIVAFATIMPTYTNNKVGTIDLMRYDS
                     KKAPSGSMDFLFLHLFAYMKEEHIQWFNLGMAPLSNVGTSRKSFLQERIAYLVYEFGS
                     HFYSFHGLREYKSKYATNWVSRYTLYSRDSWIAYVMIALLIIDNAPVEQTQGRISGLK
                     KWIRRKY"
     gene            complement(11002..11721)
                     /locus_tag="IV334_001216"
     CDS             complement(11002..11721)
                     /locus_tag="IV334_001216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAP8943298.1"
                     /translation="MKKEKKYAKMPDGSEIYYEKSGQGFPLFLLHGNDGSGRFFSEQV
                     PVLERYYTVYLVDSRGHGRSTNEASMLNFQLMAEDLNTIMLLEKIDQADFLGFSDGAN
                     LALVFASSFPKKVHRLILNSGNTLVKGVRFSARVISNIHYAWVWLLSLFRPSLRKNLL
                     VIKLLLHDIGLTENDLKKINSPTLIIVGKKDVIKLKHSLYIAKTIPKASFVLVKEQGH
                     ELARKDPERFNREVLQFLSET"
     gene            12041..12562
                     /locus_tag="IV334_001217"
     CDS             12041..12562
                     /locus_tag="IV334_001217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HAP8943299.1"
                     /translation="MESYQIEITTAAKNYHMENYENLIKAIILTESKGKGTDLMQSSE
                     SLHGEAEIIDNPADSIDQGVKYLAEMIVEAKKQGCDLWTAVQAYNFGKDYIAYVKNHG
                     GENTVELAEAYSKDVLSPLLGNEEGKQYRYWRIQSLLYNGGYLYHNGGNMFYPDIVKM
                     NEKIIETYEKVFK"
     gene            12621..13481
                     /locus_tag="IV334_001218"
     CDS             12621..13481
                     /locus_tag="IV334_001218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF368 domain-containing protein"
                     /protein_id="HAP8943300.1"
                     /translation="MEKRQKMENQEVGYTTKDWMLRFVKGMFIGSGFILPGVSGGALA
                     AVFGMYERLISFLAHLTKNFKENVLFFLPVGLGGVTGIFLLSFLVSFLLGAYETTILW
                     FFVGCIAGTVPALWKEAGKKGRDTSDVIIMVVTFVLAYLFLLYGAQLFDGQVDQNFGT
                     WLIAGGLIGLGMIVPGLSPSNFLVYMGLYKAMADGFKEVRLEVIVPIGIGGILCVLGL
                     SKIMDYIFSKAYSKLFHFILGVVFASTIMIIPTDYAGLGVAGVLACPVLFIAGTALGW
                     WMSRLEEQYK"
     gene            13588..15981
                     /locus_tag="IV334_001219"
     CDS             13588..15981
                     /locus_tag="IV334_001219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HAP8943301.1"
                     /translation="MFLILEHARLKHVIIFESWRKMEKKKMNGLTQQEVVQKMASGQQ
                     NDYQEDASKSTKQIFSDNILTLFNFLNFGIGICLLFVGAYSNMAYLAIIVVNIVIGIY
                     QEIHARNLVRKLSIVSQSEVLVIRDGKEQSISTKELVLGDLVKIGAGEQIPADLQVIS
                     GRAEANEALLTGESDLIVKETGAELLSGSFLTSGSVMAEVIRVGADNYAVRLTSEAKT
                     HKAIRSELVDSIRKVSKFTSFVIIPLGIILFLEALFIRDASIKISVIASAAALLGMLP
                     KGLVLLISIALTTGVTKLAKKRILVQDMYSIETLAHVDTLCLDKTGTITEGKMSIQRV
                     EALHPLYEKAIPEIMGTYLAESSDNNITMRAMRDYFELSNRYDVTNKVAFSSDRKWGA
                     MEFPELGVVYVGAPERLVEEDRLPDSLKTAQANGYRVLMLGIAPNQQLNEEKPAFVEP
                     VALFEIDDPIRKNAQETLAYLHEEGVDLKVISGDNPFTVSNIARRAGLPNYDAYVDLS
                     QITEEMEVREAAHRYTVFGRVSPQQKKLLVNELKESGRTVAMTGDGVNDVLALREADC
                     SIAMAEGDGATRQIANLVLLDSDFTTLPEVLFEGRRVVNNVTKVSGIFFIKTIYSFIL
                     SIICAVTAIGFPFIPIQITLIDLAIEGYPSFFLSFEQDKRKITYRYLPTALINALPNA
                     LLVVVNIIAVYLLGKTFAWGNAETTTLMYYLLIGISCMAVVKACYPFNPLRIFLAVTT
                     IVGIYVAAMLFHGILEVNLGLGESVGYFFVFMLVNIVLRVLYWKADIQNQLLKRVAN"
     gene            16163..18004
                     /locus_tag="IV334_001220"
     CDS             16163..18004
                     /locus_tag="IV334_001220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HAP8943302.1"
                     /translation="MAQRIPQEVIETVRSQTNIVEVIGQYVQLKKSGKNYLGLCPFHE
                     ERTPSFSVAEDKQIFHCFGCGKGGNVFTFLQELEGLSFPEAVVKVADFEQIPLEDQWR
                     QRTIAVQNPESATGKLIAAHEKAAEIYHHMLLHTKAGQPALEYLQSRGLSLELIEEFN
                     IGFAPNERSFLQQVFKNESISNELLERSGLFVTHDDGRMSDRFYQRVMFPLRNSQGQT
                     IGFSGRWLAPEKGQEKDQPKYLNSPETELFNKRQVLFNLDKARSDIRKNGEILLFEGF
                     MDVIAAWQAGIKTGLASMGTSLTAQQIQQMEKLTKELVFCYDGDNAGFEATNRGIELL
                     RQNSRLALSVVVVPEKLDPDEYLRKYGEASFQELVAHGRETVFTFKSRYLKQGKNLDN
                     EKDKIEYLEELLVELSRVVSPIEQDMYLSQLSTEFQIGRETIQKQLRDLRRTNQQNRQ
                     EPQESQHTQVTRQETARKKRPLTQVEKAEQILLYRAFKEVSVRNILKEREFQFIHDAY
                     AEVYFLFDAYVSQHYEFNLAEFLDFLQDENLRRLVVEIEYLPVAEESSPREIQDLLDV
                     ISKSGLADEITLKKQMQQEARRTGNKQLELELTIEIINLTKQLKQAK"
     gene            18028..19137
                     /gene="rpoD"
                     /locus_tag="IV334_001221"
     CDS             18028..19137
                     /gene="rpoD"
                     /locus_tag="IV334_001221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HAP8943303.1"
                     /translation="MANGTDKKTYEAAVAAFIKENKPKRQVVYDDLSNQLATPFTLNA
                     DEMDKLIQKIEDAGISVVDENGDPSIHSLKSAEKKAEQAKTEDLSAPTGVKINDPVRM
                     YLKEIGRVSLLTAEEEVALALKIEEGDQEAKQRLAEANLRLVVSIAKRYVGRGMQFLD
                     LIQEGNMGLMKAVEKFDYRKGFKFSTYATWWIRQAITRAIADQARTIRIPVHMVETIN
                     KLIRIQRQLLQDLGREPTPEEIGAEMDLPTEKVREILKIAQEPVSLETPIGEEDDSHL
                     GDFIEDQEATSPAEHAAYELLKEQLEDVLDTLTDREENVLRLRFGLDDGRTRTLEEVG
                     KVFGVTRERIRQIEAKALRKLRHPSRSKQLKDFLE"
     gene            19357..21456
                     /locus_tag="IV334_001222"
     CDS             19357..21456
                     /locus_tag="IV334_001222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TFIIB-type zinc ribbon-containing protein"
                     /protein_id="HAP8943304.1"
                     /translation="MKKCPNCGSKKMEQNTICPDCGHQITAEDQQIKESIEETNTDAA
                     MQDKSTEDAVFPDTELNDPIEWSELKDLPLESVMELFEKAEPEDEEPPAHDQVTNIEK
                     NEQKKATVHETSEESGKNQEETLESKQKRRVAELKEIVDNEEENSILSAYIKAHREDT
                     KEEHAKELLKMISEKIAQENKVEPEKEQETEKDSDRENATGKETVKIKDVKTDITEKT
                     EVFTEKQLEKEKTNKLPEAEKKEILENTDKKSTEPDEINSESDAENQSVSKEGTDLEA
                     KIEKEENQPKLFEAEAKSITIVPDTEKTHQESNLEKTDPDKAQKKKSKKVPYLVLAAV
                     LLLGGGSWAYYDHHQKVEAEVAAEAKRQQQMEELHEKLAAFYTDDHHQFIRADMIHQD
                     LSKLKNDLSSLKNEKGYSELETTYQDIQSKIRDIQKVNEWFIAPAIVDDHLADDIKLK
                     ADKEIQAIETDNTDFGKLIQQAQTEAKNQYAELQTAKEKTAIVFKDGKVVDSATRDQY
                     KTAKEAVEKVKNTDLSAQLNEQLKQVDKMLTDKEKKEEEEKKKAEEAKKAAEEQAKQA
                     AQAAAAAQAAQQAAVAQAAQQEAASSTPSSSNSANQPIKSARASDIADASNPAWTWAP
                     GVQSKVIATCIQRGYIVEGGYRLEKARIENGEGYYNLYATSTKSALMKGIGESALPFY
                     IVTINCKTGWFGGNGSN"
     gene            21652..22515
                     /locus_tag="IV334_001223"
     CDS             21652..22515
                     /locus_tag="IV334_001223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HAP8943305.1"
                     /translation="MNDLLATIFTGIIIDENEKNYFVQKNGQTFGLSKSEGEHKIGEA
                     VEGFGYQNQKHENRITTQIPKSRIGHYAFGIVTATRKDLGAFVDIGLPDKDFVVSLDE
                     LPTMTELWPKKGDRLMISLRVDAKDRIWGSLADEKIFKSLSKYGTEEMKNKDVIGTAY
                     RLKLTGTYVLTEDFYLGFIHPSERYNEPRLGEQIKGRVIGVRPDGVLNLSLKPRAYEA
                     ISDDAQMILTILERSADQQINFTDKSDPEEIMKAFGISKGAFKRALGNLLKQGLITQE
                     NGVTKLVKKSN"
     gene            22620..>23074
                     /locus_tag="IV334_001224"
     CDS             22620..>23074
                     /locus_tag="IV334_001224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HAP8943306.1"
                     /translation="MVMNAISAMKKTKQQLHEAGFKLTPQREATLLVLLENEKEHLSA
                     EEIFFLVKKKSPEIGLATVYRTLEILTELKIVDKVSFNDGLARYDLRKEGAKHFHHHL
                     LCLNCGTIEEVEEDLLLEVEQVVEERYHFLVKDHRLTFHGICQECYEKE"
BASE COUNT         7770 a         4038 c         4810 g         6456 t
ORIGIN      
        1 ttaaggattg ttaaaattta atcttagaaa aaaataactg gaacaagagc tatttgtttt
       61 tacagcaaag aatggtatat ttttgtagga ggtggcttac ttgaaagaag tcgttatgat
      121 agatgctgca agaacgccga taggaaagta tcgcggaagt ttaagtccat ttactgcagt
      181 tgaattaggc acacttgtga ctaaaggatt attagataaa acaaaactga aaaaagataa
      241 aatcgatcaa gtaatatttg gaaatgtcct gcaagctggt aatggtcaaa atgttgccag
      301 acagattgct ttgaacagcg gtctccctgt agatgtccca gcaatgacga tcaatgaagt
      361 ttgcggatct ggtatgaaag cagtgatctt agcacgccag ctgatccaat taggtgaagc
      421 agaacttgtg atagccggcg gaacagagag tatgtctcaa gcacctatgc tgaaacagta
      481 tcagtcagaa acaaatgaat atggtgaacc aatttccagt atggtcaacg acggattgac
      541 tgacgcattt tcaaatgcac atatgggatt aaccgcagag aatgttgcaa cacaattttc
      601 tgtgagcaga gaagagcagg atcgctatgc cttgtcgtcc cagttgaaag cagcacatgc
      661 tgtcgaagcc ggtgtatttt ctgaggagat catcccagtc aagatttctg atgaagacgt
      721 gttatctgag gatgaagcag ttcgtggaaa tagtacattg gaaaaactgg gcacgttacg
      781 tacagtattc tcagaagaag gaactgtaac agcaggaaat gcttccccgt tgaatgacgg
      841 tgcctctgtg gtgatccttg catccaaaga atacgcagaa aataataatc tgccttattt
      901 agcaaccatc aaagaagtag cggaggtcgg tattgaccca agtatcatgg gaatcgctcc
      961 aatcaaagcc attcaaaaat tgacagatag atcaggaatg aatttatcaa caatcgatct
     1021 ttttgagatc aatgaagcat ttgcagcttc ttctatcgta gttagccaag aactgcagtt
     1081 agatgaagaa aaggtcaata tatatggagg agcaatcgca ttaggccatc caattggtgc
     1141 aagcggtgca cgtatcttga ctacattagc ttacggattg cttcgtgaac agaagagata
     1201 cggtattgct tcattatgta ttggtggagg tttaggcttg gcagtcttgt tagaagcgaa
     1261 tatggagcaa acacataagg atgtgcaaaa aaaaaagttt tatcagctta cgccatctga
     1321 acggcgcagt caattaatag aaaaaaatgt attgacacaa gaaactgcgt tgatttttca
     1381 ggaacaaaca ctttctgaag agctctccga tcacatgatc gaaaaccaag tgagtgaagt
     1441 ggaaatccca atggggatcg cgcagaattt tcaaatcaat ggcaagaaaa aatggattcc
     1501 gatggcaaca gaggaaccat ctgtcattgc agctgcaagt aatggtgcaa aaatctgtgg
     1561 aaatatctgc gcagaaacac ctcagcggct gatgcgaggg caaatcgttc tatcaggaaa
     1621 atcggagtat caagcagtga tcaatgcagt taatcatcga aaagaagagc tgattctttg
     1681 tgcaaatgaa agttatcctt caatcgtgaa acgcgggggc ggtgttcaag atatctctac
     1741 tcgggaattc atggggagtt tccatgcata cttatctatt gattttttag tggatgtaaa
     1801 agatgcaatg ggcgctaaca tgatcaactc gattcttgag agtgtggcaa acaagttacg
     1861 ggagtggttc cctgaagaag agattctatt cagtatttta agtaattttg ctacagaatc
     1921 attggcttct gcctgttgtg aaatcccatt cgaacgctta gggagaaaca aagaaatcgg
     1981 tgaacaaatt gccaaaaaaa tccaacaggc aggggaatat gcgaagcttg acccttaccg
     2041 tgcagcaacg cacaacaaag ggatcatgaa tggtatagaa gctgtagtgg cagctacagg
     2101 aaatgataca cgtgcggtta gcgcttcgat ccacgcctac gcggcaagaa atggattata
     2161 ccaaggatta acagactggc agatcaaagg ggataaacta gttggaaaac taacggtgcc
     2221 tttggctgtt gccacagtag gcggagcttc caatatcctg ccaaaagcaa aagcatcttt
     2281 agctatgtta gatattgatt cagccaagga actagcacaa gtaattgctg cagtagggtt
     2341 agctcaaaac ctggctgctt tgcgtgcatt agttacagaa ggaatccaaa aaggccacat
     2401 gggacttcaa gctcgttcat tagcgatctc aattggggct attggtgagg aaatcgagca
     2461 agttgcgaaa aaattacgtg aagcagaaaa gatgaaccag cagacagcta tacaaatatt
     2521 ggaaaaaatc cgggaaaaat aaaaaagcaa tatctttaga agaataaagc aagatttaca
     2581 gttattttat ttactgcaga tcttgctttt ttctctatca gaaaatatgt ttattattag
     2641 aattttttat agttaaggtt aggcatctta agaaatcagg caactccaag atatcttcag
     2701 gtagaagaga agtcgcctaa cattgtagga tcataggtga tttgttggac aggtcaagct
     2761 gatatttgag cctgatgtta gaaggaattt ctttttactt gctgtagaaa ttattatatg
     2821 actaaaaagg ctgggatttt aaatcccagc cttttatgac aaatagaatt tttatcgatc
     2881 agtaatcgct gtcgctgcaa attgttcgat caaaggaatc aaagtcaatt tgactttgta
     2941 tttccgttcg agtgcttttt cgataacgaa ctcattttca gtagtcatag aaatagttgc
     3001 taatttacga ggttcatcag catttttttc attaataact acttcatgtt cattttgttc
     3061 tgtgtaaaat ttaggatact cagcaacggc ttttgaaaaa tcagcaaaac ttaaagcggc
     3121 attcgtatca gttgagcgtg tattttcagt tgtgtccgct tttggttttt ccgttttttc
     3181 ttctggcaca ctgactgttt cttctgtaac tggtgtcgca acagcttgcg cagttggttc
     3241 atcgtttttc aatcgttgat cattccagta agacacgatt tcaataagaa gattgtcacg
     3301 gaatcgttcg aaaatcccgt tttctcctga acgaattctc tttaatacag tattgaagtt
     3361 gtcatcgatt ggcacgttga tccgtgtatc ttcatttgtt ttcccagcta atccgatcgt
     3421 taagatttga cggtttttgg gatcaagcat gacttgtaac ggcaaaccat aacgtcgcaa
     3481 gctctcttct tctagagaac ggaataattg tcctcttaca ccatccaatg aaggacggaa
     3541 ttgatttaat aatttttgtt tctgtttttt cttcatatgt tctctccttc gctcataccc
     3601 tttcgattat aagaaaaaaa cagccaaatg aaaaggagta atgcaaaaaa acttgcgtat
     3661 acgcaaatta caacagaaaa atttgcgtat tttcccgttt tttgaaagaa aaataaaaaa
     3721 tggaacggat tatgtatata tacataatcc gttccattct ttctattttt tcggtatcaa
     3781 ggcaaaaaca ggtagttcag gtgaataaat gacttcatgt tcgtgccagg gtttgaccat
     3841 tagcgaatga tcgaaccgtt ttttcagttc ttcgatgatt tgatcatgtg tccagtttct
     3901 tgcaggaaaa tgttggattt tctctgcatc gatcaaaaag acaaaatcgg aatgaaaagt
     3961 ctgggtaact tcttccgaga gagaattcaa gcgttcgatt ggatccataa aatcaccttt
     4021 cgacttaaaa ttgggtagta tctgggtctt ttgctttgcc gactacacca tctaactgat
     4081 cgatttgttt catttcttct tctgttaatt caaaatcaaa taattcagta ttttctttga
     4141 ttctgcttgg tgtcactgac ttaggaagag gaaggaattc gtgttgcagc gaccaacgca
     4201 aagcaacttg tgcaatcgtc ttgtcatgtg cttcagcgat ttgtttcatt tcaggcacat
     4261 cgaagatttt accagtgcct aatggactat atgcttcgag gatcatatca tgtttttttg
     4321 catactcaac tacagttgtc tgtaattcgc ctggtgcaag gaaaacttga ttgaccatcg
     4381 gcataatttt tgctgttttt gctagtgtat ctagatggtg gggtaagaaa ttgctcacac
     4441 cgattgcttt gattttacct gcttcataca gttcttcaaa tgctcgccat gtatctgcat
     4501 ttgcttgttc ccaattatca cgaaaagcaa cagggtttgg ccagtggatc aaaaacagat
     4561 ctaaataatc tgtttggagc ttcttcaatg attcttcaaa agaagacatc acaagttcat
     4621 agctgtggtt tgcattccat aattttgttg tcaagaatat ctcttctcta ggaattccac
     4681 tttccttaat cgcttggccg acgctttctt cattcttgta tccttgggca gtatcgatat
     4741 ggcgataacc tgcagccagc gcttctttaa cagaggaaac agcgacatcc ccatcaggtg
     4801 tttgccatgt gccgaatcct actaccggga tttcgtaacc atttgataga cgataagtgc
     4861 ttgttaatga attcatactt tcagctcctt ttttattcgt tcaactataa gtgtagcgca
     4921 tttttcgaaa aaaagaatcc attttgtttc tactcttgaa atttttgttt aaaagaaaat
     4981 gattttgatt ctggtatttt tgattttttt ccgtaaacta agacagaagg gaatgagaaa
     5041 atggatatct atttaaaaaa agcaattctg catattattg acagagaaac tggttctcca
     5101 gtcttttcac aaaaagagct ggatctgaca aaagaatata ttcgtgattt tcttcaaaag
     5161 aagatccaaa aaatctcttc tgctcaaaca aaaactggac aactagcaga agaaagcatg
     5221 atcgttgaat cacttaaaat cgctcaagaa aattttgtgg aagcaagtga agcaatcgtt
     5281 cagcggtggt tcgatatcta ccaagaaagt gaagaagcac ctagtgcaga tgtatttgtt
     5341 gtcttgtatg aattagatac agtgatgtac ttatctttat tgaaagtcaa ttatcgtgag
     5401 gcttacactc actttgttga agcagacgaa gcagggatcg acaaccgctt gattttgaat
     5461 cgagcaattc taggggcgaa aacacaaaaa gcagatgaag cattcgctgt gaatttgact
     5521 gattggacct acgaattaat tgaaaaaaaa tatgagtttt ctggcaaaaa agagctttat
     5581 ttttctggaa gagtgatcga aagtgttcct gcaccttctt tagaagaaaa tgtaaaagtg
     5641 atccaaaaag tagccaagca tcttggaaaa aaatttgaga cagaagaatt tgatatcatg
     5701 gctgatttga aagaagctgt ctatgatacg atagaagaaa aaggtcggtt ggatcatgag
     5761 atgattgcgg aaaaagtctt caaggaaaac atcacggcaa aaatggcttt taaagaagaa
     5821 gtacaagagc aaggatttgt cccagaagca cctcctgtaa aagaagtaca agaaatttct
     5881 gagaaaaaat ttggaaaaca aaaattcaag ctatcaaacg ggatcgaact gatcgtacca
     5941 gtagatgtat atcgcaaccc agatttgatt gaatttgtga ataatccgga tggtacgata
     6001 tccgtgatga tcaaaaatgt ggatgaagta ttgaatcgtt tataaaaatg actagaatat
     6061 taggtgttca agtaccagtg atcagttaaa tgaattgtgt caagagaagg atcggaaaag
     6121 acgaatataa caaaaaaaga gcgtgagaca aaagtaaaga acacttttgt ctcacgctct
     6181 aaacacgtat aaacggcgga agcagaagca actccttctt atttcttgga gttaaacgct
     6241 tctgtcccag cctcttttta tctaataaaa ttttaagacc atttactaat tatcgatttt
     6301 ttctgccgca tttttaggca cttcagaagc ctttatttct tcccagctta atacaccttt
     6361 acgaggattg actttgagtt taagatacgc attcatcttt aatggatgtt ctctttcttc
     6421 gcgaagcgtc acctgttttt tttcaccatc tttattataa gccgcttgct tgtaggtata
     6481 agtcgtgaat ttctctccgt tatctgcttg ggtagctgac atttcgccat tcgtcgtgat
     6541 ttttgtataa taagcttcgc cgccgtaaaa ataatgataa gctgcaaaac ttccgccgag
     6601 aaatatgcct aaaacgacaa gtacgccaat taattttttc atgtacttca cctctttcct
     6661 tattattaac gaaatagaag atttcgtcta ttgaaagttg ttggcaacag ctgaatttta
     6721 aacttttcct cattttgttc ttattgcttc aaaagaaagt taaggactat cttgaagttt
     6781 ttctcaaaaa acacattttt ttatttataa aaggtacttt tttcgcgtat aagacagacg
     6841 agtgctctta gttgactttg accaatattg acgtataata agcttagttc gaatcttaca
     6901 aaaagtaagt cggacgtatt ctttttgatt tagtcaaaaa aagactataa taaaaaggtg
     6961 agagctgaaa tttttcagtg atctatatta tttaggaggt ttgtaaatta tggcaaaaga
     7021 acattatgac agaagtaaac cacacgttaa cattggtacg attggacacg ttgaccatgg
     7081 gaaaactact ttaactgctg cgatcactac agtattaggc aaaaaaggcc tagcaaatcc
     7141 tcaagactat gctagtattg atgcagctcc agaagaacgt gaacgtggga ttacgatcaa
     7201 taccgcacac gtagaatatg aaacagaaaa acgccactac gcgcatattg atgcaccagg
     7261 acacgcggac tatgtgaaaa acatgattac aggggcagca caaatggatg gtgcaatctt
     7321 agttgtttct gcaactgacg gtccgatgcc tcaaacacgt gaacacattt tattgtcacg
     7381 ccaagttggt gtaaaatacc tgattgtttt cttgaacaaa gttgatttag tcgatgatga
     7441 agaattgatc gatttggtag aaatggaagt tcgcgagtta ttgagcgaat atggtttccc
     7501 aggcgatgac actcctgtga tcaaaggttc cgcattaaaa gcattgcaag gcgatccaga
     7561 tgctgaagct gctattatgg aattgatgga tacagtagat gaatatatcc caacaccaga
     7621 acgtgataca gataaaccat tactattgcc agtggaagac gtcttctcaa ttacaggtcg
     7681 aggaactgtt gcctcaggtc gtattgatcg tggtgctgtt cgtgtcggtg atgaggtaga
     7741 gatcgtaggg atcaaacctg aaacacaaaa agcagttgta acaggtgtag aaatgttccg
     7801 taaaacgtta gattacgggg aagctgggga taacgtaggc gtgttgttac gggggatcca
     7861 acgtgacgat atcgaacgtg gacaagtact tgctaaacca ggttccatta ctccacatac
     7921 aaaattcaaa gcagaagtgt acgtgttgac aaaagaagaa ggtggacgtc atactccatt
     7981 cttcaacaac tatcgtccac agttctactt ccgcacaact gatgttacag gaacaatcac
     8041 attgccagaa gatacagaaa tggtcatgcc tggtgacaac gtgacgattg atgttgactt
     8101 gatccatcca atcgctgttg aaaacggaac gacattctca attcgtgaag gcggacggac
     8161 agtaggttca ggtatcgtta cagagatcga agcataagaa acgcgactcg aatattttgg
     8221 taatgaaaaa tgtttaaaat aaaagagcgg tccaaacagg atttctgttt ggaccgctct
     8281 ttgttcttca attgtataag aagagattgt agaagcatga actttcgttt tacttcagtc
     8341 tcttttattc atcatgagag tgttcatcta tcggtatgca gtttttaata ctttcttcgt
     8401 atccattttt tcaaaccact tattctacct tgtgtctgct caactggcgc attatcaata
     8461 atcagcaaag caatcataac atatgcaatc caactatctc tagaatatag agtataacgt
     8521 gagacccagt ttgtagcgta cttgctttta tattctctta atccatggaa agaataaaaa
     8581 tgagaaccaa actcgtaaac caagtatgca atcctttcct gtaaaaaact ctttctggaa
     8641 gttccgacat ttgaaagagg ggccatccct aaattgaacc attggatatg ttcttctttc
     8701 atataggcaa acaaatgcaa gaacaagaaa tccatactgc cagaaggtgc ttttttagaa
     8761 tcatatcgca tcagatcaat cgttcctact ttattgttcg tgtaagtagg catgatcgtt
     8821 gcaaaagcga caatttcttc ttgggtattt ttgacaactg caataggcgc tctttgtaga
     8881 taatcttcag agaaaaagcc taaagaaaac cctttttcct ttcgtgaacc caaccaatta
     8941 tcagagatat ttttgaaagt actcatttgc tcagtagaaa atggtggttg taagacatca
     9001 aatgtaaatc cttctcgttc gatccgattc aagacagcac gagtgccctt catttttttc
     9061 cctgaagttg taaaactatt caggtctaca taagcttctt cccccatctt aatgaaatcg
     9121 tagccgaatt catgcaaaat cataacaatc tcttcgcttg tttcgtagaa aactggtaaa
     9181 tagcatagtc gatctgtttc ttcgataaaa gcttcgattg cttctggaaa atcttctttc
     9241 ttaccagaag gatcacccat tacaatacat ttattattga agcaagcgaa ttggagaaga
     9301 acagttggtt caccgtcttt ttcataggca aacatccgct tatcttttaa gaagatcaat
     9361 tgactatcgg agtttcctcc ataagttgta aggatcttca gggccttttc ttcattaaag
     9421 gcttcgccaa tctgtttctt ttctccttga aggaaatgaa caaatagaac gatcatgaaa
     9481 ctaacagcga tgatggccaa caatcccgaa aaccaaatct tttcagaagg aaataagaaa
     9541 aaggaaataa aatggtgacg ccgatgaggg aaatccggca gattgtatac cccaataacg
     9601 atgtaaagca aggacagtgc accaataatg attccatcga tcgtacgcca ctcccatgaa
     9661 tagatcaatt gttcccgaaa taattcgttt ttagaagcaa gaatgatcag aagcagaata
     9721 gacaagaaaa ccaccggtgt gaaactaaaa tcacttaata aaacataaag taaagctaga
     9781 acaatcaaga agattgtagg caaatatgct cgtttcacac gtgctgcgat ccctctgccc
     9841 ataatgatta aaagaaatcc caatagtata ctaggaaatt ggataataaa atgaaatttc
     9901 aatgggttca aactatgaag ccagcgaaac tctgtaaaag cttgcgggat agtggcagaa
     9961 agtactagca tgattccaga aaagtataat aagacaacca cgattttatg cgcaatttct
    10021 gttgctaatt gtttagggat accagaataa cgctgatcaa aagtggaacc gatatttttg
    10081 aagaaaaaga cgattccaat caaaaacgga atgatgtagt agaacaggcg atagagcaag
    10141 atccacacaa cgactatttc tcttggcaca ccaatagcgg acaacccgat aatcatcatt
    10201 acatcgaaac tcccaatttc tcctggaatc atagacacga ttcctatcac tgaagcagca
    10261 atgaataatg gaattgtttg ccacagatca atccggattt ccattaaata accaacagaa
    10321 aggaaactaa acaggacacc ggtccattcc aacaatgaaa ctagcaatag ctgtccttga
    10381 gttttaagtg aggtaccacc aaggaatcct ttctttttga aagtagtaaa taaaaagacg
    10441 acaggaaaat atagaccgcc gccgatcaac catatccaat attgcttcaa aaaaggagca
    10501 actggcgtga agactacaag taaaaaagaa agcagactgt aaatagaaag tccagccatc
    10561 aaaaacaaga aaactttcga taatgcttgg atcacgtttc tgctctcttt cttttgtcca
    10621 tatagttcag agcgaagccc cacactaaca aacccgccga aaccggcaat attgttgatc
    10681 gtattgatca gccagcttgt ctcaaacagg taacgaggat tctgcttttg ttcaagcatc
    10741 cgattcaaaa taatatcata accaaccata ggtaacacag agataagacc aatcaatagc
    10801 attagtccgc ttttccatac aggtatagtg gcaaacgttt ctcctaattg ctggatagat
    10861 agagttttac cgatggacat caattcaccg acaataatga caatgacaga aatcaaaaag
    10921 attgttttga acagtcctag gtggtttttc agccattgta tggtattttt taacaaataa
    10981 atcaccttct attcctaata gttatgtttc agaaagaaat tgaagaactt ctcgattgaa
    11041 gcgttcagga tcttttctag ctaattcatg tccttgttct ttcacaagca caaaggaagc
    11101 ttttggtata gttttagcta tatacaaaga atgttttagc ttgattacat ctttttttcc
    11161 cacgatgatc aaagtggggg agttgatttt ttttaaatca ttttctgtaa gcccgatgtc
    11221 atgcagcaaa agtttgataa ctaaaagatt tttgcgaaga cttgggcgaa ataaagagag
    11281 taaccatacc catgcataat gaatattact gatgactctg gcagaaaaac gaacgccttt
    11341 aacaagcgtg tttccagaat ttaaaatcaa tcgatgaact tttttaggga agctgctggc
    11401 aaataccagt gctagatttg ctccatcact aaatccaaga aaatctgcct gatcgatttt
    11461 ttccagcaac atgatcgtat tcagatcctc ggccattaac tgaaaattca acatagatgc
    11521 ttcgtttgtc gaacgaccat gtcctcggct atctactaga tacacggtat agtagcgttc
    11581 gagtacaggg acctgttctg aaaaaaagcg gccgctgccg tcattgccat gcagtaaaaa
    11641 gagagggaag ccttgaccgc tcttttcata gtaaatttcg ctcccatctg gcatttttgc
    11701 atatttcttt tcttttttca cataatcacc tactgtgtaa agtataacga ttatatcagt
    11761 cattgggcaa ttgtttttac tgaaaaacaa cgatcatttt cgtaagatgt caaaaaatac
    11821 aggaaaattc attttttctt aaggcgtaca cagaaatcaa ggaagaaata gccaagaaga
    11881 taaagaaata gtaagataaa aaaagatgtc aaaataacta ggtgatgaaa tgaagcgaaa
    11941 acgattatct attatccgta ttctttttta catgatcatt ctgacggtta ttggtgcggt
    12001 gttgttttta tgaggaagaa cttatcgttc ggtaaaacga gtggaaagct atcaaataga
    12061 aataacaaca gctgcaaaaa attatcatat ggaaaactac gagaatttga tcaaggcgat
    12121 aatattgacc gaatcaaaag gaaaagggac agatctcatg cagagctcgg aaagtctgca
    12181 tggagaagct gagatcattg ataatccagc tgacagtatt gatcaaggtg tcaaatattt
    12241 ggcggaaatg atagtagaag caaagaaaca aggatgcgat ttatggacag ctgtccaagc
    12301 atataatttt gggaaagatt atattgccta tgtaaaaaat catggcggag aaaacacagt
    12361 agaattagca gaagcgtatt ctaaagatgt gctgagccca cttttaggaa atgaggaagg
    12421 gaaacaatac cgatattggc ggatccagtc cttgctttac aatggcggct atctctatca
    12481 taatggaggt aacatgtttt atcctgatat tgtcaaaatg aatgaaaaaa taatagagac
    12541 ctatgaaaaa gtctttaagt aaaatagcag gaaatgttca atcatgaata aaattaaatg
    12601 aaagaaggag tgcgcaaata gtggaaaaaa ggcaaaaaat ggaaaatcaa gaagtggggt
    12661 acacaaccaa agactggatg ttacggtttg tgaagggaat gtttatcggt tcaggattta
    12721 tcttgccagg agtcagcggt ggtgcattag cagctgtctt tggcatgtat gagcggctga
    12781 tttctttttt agcccacctt acaaaaaatt tcaaagaaaa tgttttgttt tttctgcctg
    12841 ttggtctagg cggagtgaca ggcatctttc tactttcttt tttagtcagt tttttattag
    12901 gtgcgtatga aacaacgata ttatggtttt ttgtcggatg tatcgctgga actgttcctg
    12961 cgttatggaa agaagcaggg aaaaaaggaa gagatacttc tgatgtgatc atcatggttg
    13021 ttacttttgt tctggcttat ttgtttttat tatacggcgc tcaattattt gacgggcaag
    13081 tcgatcagaa ttttggaaca tggctgattg ccggcggatt gattggtttg ggtatgatcg
    13141 tcccaggatt gagcccttct aattttttgg tgtatatggg attgtataaa gcgatggctg
    13201 acggatttaa agaagtacga cttgaagtga ttgtgccgat cggaattggt gggattcttt
    13261 gtgttcttgg attgtcaaaa atcatggatt acattttctc aaaggcatat agcaagctat
    13321 tccatttcat tttaggtgta gtttttgctt caacgattat gatcattcct accgattacg
    13381 cgggattggg agttgctggg gtactagctt gtcctgttct ttttatagcc ggtacagcac
    13441 taggttggtg gatgagccgt ttggaggaac aatataagta aagatagatt gataataaaa
    13501 acagcacacg ggtgattcaa acaaaaaggt tggcatcttt tttgtaaaat cattcgtgtg
    13561 tttttctata aattttataa aaacaagttg tttttaatct tggaacacgc tagacttaaa
    13621 cacgtgatta tttttgaaag ttggcggaaa atggagaaaa agaaaatgaa tggacttaca
    13681 cagcaagagg tagttcagaa aatggcttct ggccagcaaa atgactacca ggaagatgca
    13741 tcaaaaagta caaaacaaat attcagcgat aatatattga cgttattcaa ttttctgaac
    13801 tttggtattg ggatttgtct gttatttgta ggtgcgtatt ccaacatggc gtatttagca
    13861 attattgtcg tcaatatcgt tattggaatt tatcaagaaa tacatgcaag aaatcttgta
    13921 cggaaattat cgattgtttc ccaatcagaa gtactagtga tccgggacgg taaagaacaa
    13981 tcgatttcta ctaaagagct ggtattagga gatttggtaa aaatcggagc aggagaacag
    14041 ataccagccg atttacaggt tatatctgga cgggcagaag caaatgaagc tcttttgaca
    14101 ggcgaatctg atttgattgt caaagaaact ggagctgaat tgctttctgg aagctttttg
    14161 acaagcggtt ctgtcatggc tgaagttatc cgagtaggtg cagataatta tgctgtccgc
    14221 ttgactagcg aggcaaagac tcacaaagct attcgttctg aacttgtaga ttcgattcgc
    14281 aaagtatcca aattcactag tttcgtgatt attcctttgg ggatcatact atttttggaa
    14341 gcactgttca tccgcgatgc ttctatcaaa atttctgtta ttgcatctgc agcagctttg
    14401 ttaggcatgc ttccaaaggg tttggtattg ctaataagta ttgcattaac gacaggggta
    14461 accaaactgg caaaaaaacg aatccttgtt caagacatgt attcaattga aacgctggcc
    14521 catgtcgata cattatgctt ggataaaaca gggaccatta ctgaagggaa aatgagtatc
    14581 cagcgggtag aagcactaca tccgctgtac gagaaagcca tcccagaaat tatggggacg
    14641 tatctggcag aaagcagtga taacaacatc actatgcggg ctatgagaga ttattttgag
    14701 ctttctaatc ggtatgatgt gacaaacaaa gtcgcatttt cttctgatcg caaatggggc
    14761 gcgatggaat ttccagaact gggagtcgtg tacgtaggag cgcctgaacg tttggtggaa
    14821 gaagatcggc ttccggacag tctaaaaaca gcccaagcga atggctatcg cgtacttatg
    14881 ttggggatcg ctccaaatca gcaattgaac gaagaaaaac cagcatttgt tgagccggtt
    14941 gctctgtttg aaatcgatga tccaatccga aaaaacgctc aagaaacact agcttatctt
    15001 catgaggaag gtgtggatct gaaagtgatt tctggagaca atccttttac tgtctccaat
    15061 atcgctagac gtgcaggatt accgaattat gatgcgtatg tcgatctttc tcaaattact
    15121 gaagaaatgg aagtgcgaga agctgctcat cgctatacgg tttttggacg agtgagtccg
    15181 cagcaaaaga aattgctggt aaatgaattg aaagaaagcg gcagaacggt tgcaatgaca
    15241 ggcgacggtg tgaatgatgt tttggcgtta agagaagcag attgcagcat tgctatggca
    15301 gaaggtgatg gagcaacacg tcagattgcg aatctggtat tgttggactc tgattttact
    15361 acattgccag aagtcctgtt tgaaggacgc cgagtggtca acaatgtgac gaaagtctca
    15421 ggcatctttt ttatcaaaac gatttattca tttattttat cgattatatg tgccgttaca
    15481 gcaattggat ttccgtttat ccctatccaa atcacgctga ttgatttagc gattgaaggg
    15541 tatccttcct tctttttatc ttttgaacaa gataaaagaa aaatcactta ccgttatttg
    15601 cctacagcac ttattaatgc tttaccaaat gccttgcttg tagtagtgaa tatcattgcg
    15661 gtgtatctac ttggaaaaac attcgcatgg ggaaatgctg aaactactac tttgatgtat
    15721 tacttgctga ttgggatcag ttgcatggca gttgtaaaag cttgttatcc cttcaaccca
    15781 ttgcgcatct tcctagcagt aacgacgatc gtagggatat atgtagctgc aatgctgttc
    15841 catggaattt tggaagtaaa cttagggttg ggagagagcg taggatactt ctttgtattc
    15901 atgctcgtaa atattgttct tcgagtattg tactggaagg ctgatattca aaatcagtta
    15961 ctgaaaagag tagcaaattg atgcttaatg agatagaaat gtgttatact tgaaaaagca
    16021 atgaaatatg atacaatcaa acatgtataa ttgtgtcctt taaaatcgca ctgatttcag
    16081 ttagtgcgat tttttaccgg ttattctcct gaaatatctt ctgttaaacc caaaagatat
    16141 caatagagca gggggtgaaa aaatggctca acggattcct caagaggtta tcgaaacagt
    16201 acgcagtcaa acgaatatcg ttgaggtgat tggacaatat gtccagttga aaaagtctgg
    16261 taaaaattac ttggggctgt gtccttttca tgaagaacgt acaccgtctt tttctgttgc
    16321 agaagacaag cagatttttc attgctttgg ttgtggtaaa ggcggaaacg tgtttacttt
    16381 tcttcaagaa ctagaaggtc taagttttcc agaagcagtg gtaaaagtgg cagacttcga
    16441 acagataccg ttagaagatc aatggcgtca gcgtactata gctgtccaaa atcctgaatc
    16501 tgctacgggg aaactgattg ctgctcatga aaaagcagcg gaaatctatc atcacatgct
    16561 attgcataca aaagctggac agccagcttt agaatatttg caaagtcgtg ggttgtcttt
    16621 agaattaatt gaagaattca acataggttt tgcacctaat gagcgttctt ttcttcagca
    16681 agtattcaag aatgaatcaa tttcaaatga attgttagaa cgctctggat tgtttgtcac
    16741 gcatgatgat ggccgtatgt ctgatcgttt ttatcaaaga gtgatgtttc cacttcgtaa
    16801 ttctcaaggc cagacgatcg gtttttcagg acgctggctt gcacctgaaa aagggcagga
    16861 aaaagatcag cccaaatact tgaatagtcc agaaacagaa ctgttcaata aaagacaagt
    16921 tttatttaat cttgataaag caagaagcga tatccggaaa aacggagaaa ttttgttgtt
    16981 cgaagggttt atggatgtga ttgctgcatg gcaggctggt atcaaaactg gattagcttc
    17041 tatggggaca agtctaacag ctcaacagat ccaacaaatg gaaaagctga cgaaagaatt
    17101 ggtcttttgc tatgacggag acaacgcagg ttttgaagca accaatcgag ggattgaatt
    17161 gcttcggcaa aacagtcgat tagcactttc tgtcgtagtc gttccagaaa agcttgatcc
    17221 tgatgaatat ctccgtaagt atggagaagc atctttccaa gaattagtgg ctcatgggcg
    17281 cgaaacagtc tttactttta aatcacgtta tttaaaacaa gggaaaaatt tagataatga
    17341 aaaagacaag atcgaatacc tcgaagaatt actagttgaa cttagccgag ttgtttcacc
    17401 aatcgaacaa gatatgtatc tatctcaact gtcaacagag tttcagatcg gcagagagac
    17461 gatacaaaaa caactgcgcg atcttcgaag gacaaatcag caaaatcgtc aagaacctca
    17521 ggaaagtcaa cacactcagg ttacgagaca agaaactgca cggaaaaaga gacctttgac
    17581 gcaagtagaa aaggcagagc agattctatt atatcgtgca tttaaagaag tttctgtccg
    17641 taatatttta aaagaacgag aatttcagtt tattcatgat gcatatgcag aagtttattt
    17701 tttattcgat gcatatgtct cacaacatta tgaattcaac cttgcagagt ttttagactt
    17761 tttgcaagat gaaaatttaa ggcggttggt ggtggaaatc gaatatttac cggtggcaga
    17821 agaaagttcg ccacgagaaa tccaagactt gctagatgtg atcagcaaat ctggcttagc
    17881 tgatgaaata acattaaaaa aacaaatgca gcaagaagcc agacgaactg gaaacaaaca
    17941 gcttgagctt gaattgacga ttgaaatcat caatttaacg aaacagttaa aacaagccaa
    18001 atagctactc aaggggggcc ttcttttatg gcaaatggaa cagataaaaa aacgtatgaa
    18061 gcagcagtag cagctttcat caaagaaaac aaacccaaac gacaagttgt atatgatgat
    18121 ttatccaatc agcttgctac accattcact ttgaatgcag atgaaatgga caaattgatc
    18181 caaaagattg aagacgcagg tatcagtgtg gtggatgaaa atggtgatcc atcaattcac
    18241 agcttgaaga gtgctgagaa aaaagctgaa caagcaaaaa cagaagatct ttctgcgcca
    18301 accggtgtaa aaatcaatga cccggttcgt atgtatctga aagaaattgg ccgtgtctcc
    18361 cttttaactg cggaagaaga agtagcgtta gcattgaaaa tcgaagaagg cgaccaagaa
    18421 gcgaaacagc gtctggcaga agcaaacctt cgtttagtag taagtatcgc taaacgttat
    18481 gtaggacgag gcatgcagtt ccttgacttg atccaagaag gaaacatggg actgatgaag
    18541 gcagttgaaa aatttgacta ccgcaaagga tttaagtttt caacctatgc tacatggtgg
    18601 attcgtcaag ccatcactcg agcaatcgct gaccaagcac gtacgatccg tattccagtt
    18661 catatggttg aaacaattaa taaattgata cgtatccaac gtcaattgct gcaagacctt
    18721 ggaagagaac ctacgccaga agaaatcggt gcggaaatgg atcttccaac agaaaaagtc
    18781 cgagaaatct tgaaaatcgc gcaagaacct gtttcccttg aaacgccaat cggtgaagaa
    18841 gatgactctc acttaggtga tttcatagaa gaccaggaag caacaagtcc agcagaacac
    18901 gcagcttatg agttactaaa agaacaattg gaagatgtac tagatacatt gactgatcgg
    18961 gaagagaatg tccttcgctt acgttttggt ttagatgatg gccggacgag aacattggaa
    19021 gaagtaggga aagtatttgg tgttactcgg gaacgtattc gtcagatcga ggctaaagca
    19081 ttaagaaaat tgcgccatcc ttctcgttca aaacaattaa aagatttctt agaataagcg
    19141 tggactattg accagaagag aaatcagtaa aactgtttgg tttattttaa aagaaaaaaa
    19201 gcttgagact acgcagtctc aagctttttt ttctatagga aggaaattca ttcattattt
    19261 ttaagtaaat cctaaaaata agctattggc gtgacgtata gaatatgata gattaaatgg
    19321 tagcgtaaac ctagtaaagg agccgatcgc taagtgatga aaaaatgtcc aaattgcgga
    19381 tcgaaaaaaa tggagcagaa tactatctgt ccagactgcg gtcatcaaat aactgcagaa
    19441 gatcagcaaa taaaagaatc aattgaagaa acaaatacag atgcagctat gcaggataaa
    19501 tcaactgaag atgcagtctt tcctgataca gaattaaacg atccaatcga atggtcggaa
    19561 ttgaaagatt tacctctaga atctgtaatg gaattattcg aaaaagcaga accagaagac
    19621 gaagaaccac ctgctcacga tcaagtaact aatatagaga aaaatgaaca gaagaaagca
    19681 actgttcatg agacttcgga agaatcagga aagaatcaag aagaaacgct tgaatcaaaa
    19741 caaaaaagaa gagtagctga actgaaagaa atcgtcgata atgaggaaga aaattcgata
    19801 ctatccgctt atatcaaagc acatcgtgaa gatacaaaag aagagcacgc gaaagagctg
    19861 ctcaaaatga tcagtgaaaa aattgcacag gaaaacaaag tagagccaga aaaggaacaa
    19921 gaaactgaaa aagactctga tagagaaaac gccacaggta aagaaacagt aaaaataaaa
    19981 gacgtaaaaa cggatatcac agaaaaaact gaagtattta ctgaaaaaca attagaaaag
    20041 gaaaaaacga ataaactgcc ggaagcagag aaaaaagaaa tactcgaaaa cacagacaaa
    20101 aagtcaacag agcccgatga aataaatagt gaatccgatg ctgaaaatca atctgtaagc
    20161 aaagagggta ctgatcttga agcaaaaata gaaaaagaag agaaccagcc taagctgttt
    20221 gaagcagaag cgaaatcaat aacaatcgtg ccagatactg aaaaaacaca tcaagaatca
    20281 aacttagaaa aaacagatcc agataaagcg caaaagaaaa aatcaaaaaa agtaccttat
    20341 ctcgttttag cagcagtact tctactcggt ggaggaagct gggcttatta tgaccatcat
    20401 caaaaagtag aagccgaagt agcagcagaa gcaaaaagac agcaacaaat ggaagaactt
    20461 catgagaaac ttgctgcctt ttatacagat gatcaccatc aatttattcg tgcagatatg
    20521 attcatcaag atttatcaaa actaaaaaat gatttatcca gtcttaagaa tgaaaaagga
    20581 tattcagagt tagaaacaac ttaccaagat atccaatcta agatccgaga tatccaaaaa
    20641 gtgaatgagt ggttcatagc acctgccatc gtcgatgatc atctagcaga tgacatcaag
    20701 ttgaaagcag ataaagagat ccaagcaatc gaaacggata acacagattt tggaaaattg
    20761 attcaacaag cacaaacaga agctaaaaat caatatgctg aattgcaaac agctaaagaa
    20821 aaaacagcta tagtctttaa agacggaaaa gtagtggatt cagcaacacg cgaccaatac
    20881 aagacggcaa aagaagcagt agaaaaagtg aaaaatacag atctatctgc tcagttgaat
    20941 gaacaattga aacaagtaga taagatgctc actgataaag agaaaaaaga agaagaggaa
    21001 aagaaaaaag cagaagaagc gaaaaaagca gctgaagaac aagcaaaaca agccgctcaa
    21061 gcagcagctg cagctcaagc cgctcaacaa gctgcggtcg cacaagcagc acagcaagaa
    21121 gcggcatcct cgaccccttc ctcttcaaac tcagcgaatc agccaatcaa atctgccaga
    21181 gcaagtgata ttgccgatgc atctaatcca gcatggacgt gggcaccagg tgtccaaagc
    21241 aaagtgattg ctacatgtat ccaacgtgga tatatcgttg aaggaggtta cagattagaa
    21301 aaagccagaa tcgaaaatgg ggaaggctat tataatctat atgccacttc aacaaaatct
    21361 gcattgatga aaggcattgg agaaagtgct ttgccatttt acatcgtaac catcaactgt
    21421 aaaactggat ggttcggagg aaatgggagc aattaatttt aggcaaatga aagaggctgt
    21481 gagcctgccg tttaacaccg tttattcgaa aaaggggagt atgacacaac tgttgtcata
    21541 ctcctttttt cacctttcca aaccaaggct cagctattca agtagataac attatgttat
    21601 gatagcaaag aaatgtatta agaaaacctt tcactataga gggagaaaaa aatgaacgat
    21661 ttattagcaa ctatatttac aggtattatt attgatgaaa atgaaaaaaa ctactttgtc
    21721 caaaaaaacg gacaaacctt tggcttaagc aaatcagaag gtgaacacaa aatcggtgaa
    21781 gcagttgaag gattcggtta ccaaaatcaa aaacatgaaa atcggatcac tacacaaatt
    21841 cctaaaagcc gtatcggtca ttacgctttt ggaatcgtta ctgctacacg aaaagattta
    21901 ggtgcgtttg tcgatatcgg tcttccagat aaagactttg tcgtatctct tgatgaattg
    21961 ccgacaatga cagagctatg gcctaaaaaa ggggatcgct tgatgatttc ccttagagtt
    22021 gatgcaaaag accgtatttg gggcagttta gcagatgaaa aaatattcaa atctttaagc
    22081 aaatatggaa cagaagaaat gaaaaacaaa gatgtgatag gaacagcata ccgcttgaaa
    22141 ttaacaggta cgtacgtttt aacggaagat ttctatcttg ggtttatcca tccttctgaa
    22201 cgatataatg aacctcgtct aggcgagcag ataaaaggtc gagtgattgg tgtccgacca
    22261 gacggtgtac tgaatctttc attgaagcca agagcatatg aagctatatc agatgatgca
    22321 caaatgattt taaccatttt agaacgttct gcagatcagc aaatcaactt tacagataaa
    22381 tcggatccag aagaaatcat gaaagctttt ggtatcagca aaggagcatt taagcgcgca
    22441 ttaggtaatc ttttgaaaca aggattgatt acacaagaaa acggtgtgac aaaactagtc
    22501 aaaaaatcta attgagaatc ccttgcatgt ttttcttaac tagcttataa taattttaat
    22561 taacatagtg ttatatcttt ttttatttgt aattattata aattgttggg gttgagtaaa
    22621 tggtcatgaa tgctatttcg gcgatgaaaa aaacaaaaca gcagctacat gaagcggggt
    22681 ttaagctgac accgcaaaga gaagctactc tgcttgtgct tttggaaaat gagaaagagc
    22741 atttatctgc agaagaaatc ttctttttag tgaaaaaaaa gagtccggaa attggacttg
    22801 ctactgttta tcgaaccctt gaaatcttga cagaattgaa aattgtcgat aaagtaagtt
    22861 ttaatgatgg attagcccgt tatgatttga gaaaagaagg cgccaaacat ttccatcatc
    22921 atttactatg cttgaactgc ggcacgatcg aggaagtaga agaggatctt ttacttgaag
    22981 tggagcaagt agtagaagaa cgttatcatt ttttagtaaa agatcatcgg ctaacgttcc
    23041 atggaatctg tcaggaatgc tatgaaaaag aaaa
//
LOCUS       DACDKM010000033        23065 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20834_2016
            SAMN12640370-rid9891663.denovo.033, whole genome shotgun sequence.
ACCESSION   DACDKM010000033 DACDKM010000000
VERSION     DACDKM010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640370
            Sequence Read Archive: SRR10040891
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23065)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23065)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742835.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742835.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:04:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,012
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 29 of 75
            Pseudo Genes (incomplete)         :: 33 of 75
            Pseudo Genes (internal stop)      :: 19 of 75
            Pseudo Genes (multiple problems)  :: 5 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23065
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640370-rid9891663.denovo.033"
                     /isolate="E20834_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(109..564)
                     /locus_tag="IV334_001225"
     CDS             complement(109..564)
                     /locus_tag="IV334_001225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8943307.1"
                     /translation="MSKEMTALKFYFRNGETWTINRRHIGDLWIKQITTSFGRINGSE
                     FVEIHPCAGFKIEIFHEGDAVATHDINLGGLEMGMFNRALKYEDIERMEILYRNGTPD
                     LVYFPYLDKGTEGLDNQYQSTKISEKTGNLYIVINPDQRVEDVYGEFFE"
     gene            complement(718..1926)
                     /locus_tag="IV334_001226"
     CDS             complement(718..1926)
                     /locus_tag="IV334_001226"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CCA tRNA nucleotidyltransferase"
                     /protein_id="HAP8943308.1"
                     /translation="MKLKQLPLEYQKAIPVLKKLENAGYEAYFVGGSVRDILLNQPIH
                     DVDIATSAFPEEIKQLFPKTIDIGIEHGTVLVLENDEQYEITTFRTESTYQDYRRPDH
                     VEFVRSLAEDLKRRDFTINAFALKEDGEIIDLFDGLQDLEEQILRAVGNPHERFHEDA
                     LRMMRGLRFVSQLGFKLEEETFYAIKENHELLGKISVERINVEFIKMLLGNEGAAGIH
                     AFIETECYICCPGLKEEGEALLRFAELPSVPLKEESQAWALLISQLDLKENEIRSFLK
                     SWKCSNQMIKDVQALVQGLRQRETGALEPFELYFLGKKHALQVEELMIYFDETPQMDA
                     VGESYDRLPIKSLSELAVDGKILLEETGKKPGKWVSEALQLAEKAVVEQRIKNDKKQV
                     LDFLSKEKLI"
     gene            complement(2038..2370)
                     /locus_tag="IV334_001227"
     CDS             complement(2038..2370)
                     /locus_tag="IV334_001227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HAP8943309.1"
                     /translation="MDKKRSLYSMQQEVDEYIQQFKVGYFSPLAQMARLTEEVGELAR
                     EVNHTYGEKSKKASEPVNSVAEELGDVLFVTMIMANSLNIDLTEVFEKNMEKFNQRDH
                     NRFERKDE"
     gene            2518..3393
                     /locus_tag="IV334_001228"
     CDS             2518..3393
                     /locus_tag="IV334_001228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HAP8943310.1"
                     /translation="MVKRKTVKEVLFIIIGTSIYAFGLVYLNIANHLAEGGVSGITLI
                     LRALFGIDPAYTTLLINIPLILLGGKILGKRSLAYTVLGTLSLSFFLWIWQRVPLEIN
                     LQHDLLIVSLLAGLVAGVGSGIVYRMGGTTGGSDIIARILEQNYGITMGRSLLAFDII
                     VLLASLTYIDLKRMMYTLIVSYVFSRVIDSILDGGYSAKGLLVVSNKSEEIAPLLMTG
                     LQRGVTFLSGEGAFSGNSKKIIYMVVSSSELTEAKRIVHEIDEKAFLSIINVHEVEGQ
                     GFTYLKPQTRLLKKL"
     gene            complement(3475..4056)
                     /locus_tag="IV334_001229"
     CDS             complement(3475..4056)
                     /locus_tag="IV334_001229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IDEAL domain-containing protein"
                     /protein_id="HAP8943311.1"
                     /translation="MLIDVREKKNFLTWMVNHVSFSRREVFWILNYLANHEAILSNVH
                     FVEGANFADRGLQISDLSVEGEPMKLFLQGKEFTDTDQIFHEIRLNWKKPLYVECIFE
                     NAWQTREYLSILEDNPFSSWDSSVSEEDTELIERYFQEKEEEAKLKMLYAQIDQALED
                     GDRDAFLKLSDEVNRKILLQTQKKSNRVSEHNN"
     gene            complement(4062..5321)
                     /locus_tag="IV334_001230"
     CDS             complement(4062..5321)
                     /locus_tag="IV334_001230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HAP8943312.1"
                     /translation="MNTNSEKMLQALSDEDLSQAQIFLEKALKEDPADILAELGEELL
                     AIGFLIEAKQIFEQLINQYPENDGLNIPLAEIAIEDNEIDLAFEYLEKVDKQSDYYPQ
                     SLLAIADLYQVIGIPEVSEAKLKEAAGLLPDEPLIQFALAELYFSVDRFKEAAVVYET
                     LLASGINEISGISMQERLGQSLSMQGEFEAAIVPLEEALKEERTDDRLFQLAFTNLQL
                     KENQQAINYLEELREVNPHYQSLYLYLGQALQEEEMIEEAQTVLEEGIKENPYQVELY
                     HLASENAFRLHDRQRAEELLLKALEVGDKQDETLLTLSNLYLDDERYEDVIQVINQME
                     ETANPYAEWNLAHAYNELEDFTVAAVHYEQAYHELSHEPDFLKEYAFFLREEGQLKRA
                     QELLTYYLTLEPGDMEALSLLDDLTER"
     gene            complement(5323..6369)
                     /locus_tag="IV334_001231"
     CDS             complement(5323..6369)
                     /locus_tag="IV334_001231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8943313.1"
                     /translation="MELDYQVEDALTKVFYGETLVSQLRELSIDQPILFLTNQRYYDL
                     FADKINPLFANQANNDWYICANTQCNHLTELKNLLDFVKRYPENQSMFIIGFGNEGIM
                     DLAGFFQKHTTLDSSLWMIPVSIRSLSRALTPQRTILQLPNRVVLQTINLPERIVYDQ
                     TISERQVDGKQIDFLTFICCGMICDHRFLQNLYKNYPNKKQLTTRPFAGMIEEMIYFY
                     QLEAENLSNYGKLFEQAFYLTENGHLLSASMKKFLGMMFHFVWNLQAYPFDFQLKNFF
                     VWLDLLGYPIHWLEQISKMEYLEKVLFLAEKSKKILVLEKIGIIDGYQHPNEKELLRT
                     MTVFQQIVSEIRGN"
     gene            complement(6604..6879)
                     /locus_tag="IV334_001232"
     CDS             complement(6604..6879)
                     /locus_tag="IV334_001232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008214712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HAP8943314.1"
                     /translation="MANKAELIEKVASATDLTKKDATAAVDAVFSTIQDALANGEKVQ
                     LIGFGNFEVRERAARKGRNPQTGEEIEIPASKVPAFKPGKALKDAVK"
     gene            complement(7122..8432)
                     /locus_tag="IV334_001233"
     CDS             complement(7122..8432)
                     /locus_tag="IV334_001233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HAP8943315.1"
                     /translation="MANPTIAIVGRPNVGKSTIFNRIAGERISIVEDTPGVTRDRIYA
                     KGEWLGREFSVIDTGGIDLGDEPFMDQIKHQAEIAIEEADVIICVVSGREGVTDADEM
                     VAKILYRSNKPVILAVNKVDNPEMRNDIYEFYSLGLGDPYPVSGSHGLGIGDILDEAV
                     KYFSEETEEEDDDTIKFSLIGRPNVGKSSLINAILGEERVIVSDIEGTTRDAIDTHFV
                     SESGQKFLMIDTAGMRKRGKVYENTEKYSVMRAMRAIDRSDIVLMVLNAEEGIREQDK
                     RVAGYAHEAGRGIIIVVNKWDLVKKETNTMRDFEQEIRDEFRYLDYAPIVFVSAVTKQ
                     RLERLPEMIEQVSMNQNLRISSAVLNDIIMDAVAINPTPTDKGKRLKIFYATQVAVKP
                     PTFVVFVNEEELMHFSYERFLENQIRKAFTFEGTSIRIIPRRRK"
     gene            complement(8614..9843)
                     /gene="rpsA"
                     /locus_tag="IV334_001234"
     CDS             complement(8614..9843)
                     /gene="rpsA"
                     /locus_tag="IV334_001234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HAP8943316.1"
                     /translation="MTEFDQNQENNQSMEDAMKSVQEVKVGDVVKGEVLVIEDKQVIV
                     GIEGAGVEGVVPAKELSTLPVEDISEVVKVGDVLDLVVISTIGKDKENGSYLLSKRRL
                     DAKKVWEDIERDFQEGKVIEAPVTNVVKGGLVVDVGVRGFVPASMVEDHFVADFSDYK
                     GKTLSFKIIEIEPSENRLILSHKAVVENEREEKKKEILSTIHEGDVLSGRVARLTDFG
                     AFVDLGGIDGLVHVSEISHSHVAKPSDVLAVNDDVNVKVLSINPEQERVSLSIKDTQP
                     GPWSEIDTKAPVGTVLDGTVKRLTSFGAFVEVFPGVEGLVHISQISHKHIATPHEVLH
                     EGDEVKVKVLEIHPEEHRIALSIKALEEKPASEEEPKNVESYELPEENTGFTMGDILG
                     DALKEEVEEKATDDSEK"
     gene            complement(9978..10658)
                     /locus_tag="IV334_001235"
     CDS             complement(9978..10658)
                     /locus_tag="IV334_001235"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HAP8943317.1"
                     /translation="MTQISIAIDGPASSGKSTVAKILAKELNYIYTDTGAMYRAVTYL
                     AITNNVSFSDEEGLVSLIKKYPISFSQQEDGQHVFIASKDVTLEIRQPDVTKAVSEVS
                     AHGKVREELVAVQRKIGERGGVVMDGRDIGTAVLPNAEVKIFLVASVSERAERRFKEN
                     QEKGIPTDLETLTKEIQERDHYDSTRDVSPLKQAEDAVRIDTTGKSIPEVVAAIKAVV
                     LKKGYLLF"
     gene            complement(10707..11342)
                     /locus_tag="IV334_001236"
     CDS             complement(10707..11342)
                     /locus_tag="IV334_001236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HAP8943318.1"
                     /translation="MSRKDRHQSTENNETQEPWEQPIYDTDDEVSSRSSQRQQKKGNT
                     LFLSLFLILLVLCIAIPAGAYFWIRNGSSNNATAATSSETSTSVVESSTSTTKEESTE
                     TSTVESSTIAEESTVSQDTGVTSESSIAAETTPTSSSEATYQAGDSSSNAAETTPSSS
                     AAAGSTTTVQAGEGPKQVAERAGITTDQLFELNGLDPNNFMLYPGQELRIK"
     gene            complement(11408..12811)
                     /locus_tag="IV334_001237"
     CDS             complement(11408..12811)
                     /locus_tag="IV334_001237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecQ"
                     /protein_id="HAP8943319.1"
                     /translation="MLINELKKYFGFDSFRPGQEEIIQALLDGEDTLAILPTGTGKSL
                     CYQLTGYLMEGLVIIVSPLLSLMEDQVTQLQKRGEKRVAAFNSLLSQSERRYVLRHLS
                     QYKFLFLSPEMLTNPSLLEHLKKQNIALYVVDEAHCVSQWGVDFRPEYQQLGKIRKLL
                     GNPVTLALTATATDLVARDIRHVLFDRPPKEIRQSVNRQNISLFVRKTQQKEQELEHF
                     MERAQGAAIIYCATKKEVERLYHLFRERFTVGYYHGGLDAAQRRQLQQQFVKNQLQFL
                     IATNAFGMGIDKSDIRYVVHYDLPDSLENYVQEIGRAGRDQEESAAILFYQSGDERIH
                     YFFNQLSREQRQSFELYLEYATKQAPFDELQKKWMELIQQSEKPENWVERLKRQEKEK
                     EFRLQQMLRYINEENCRRKFILAYFGEKLSEKPQNCCDIDGAKAELLPKRKLFSKTEQ
                     LHWETILLNLFKKNMEH"
     gene            complement(12798..13829)
                     /locus_tag="IV334_001238"
     CDS             complement(12798..13829)
                     /locus_tag="IV334_001238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8943320.1"
                     /translation="MIVFILSLFSSGHKLRISSLYQLLVGKRTTSVLIYGFTHELLFI
                     HNSFPELKQDKFYQILQKIAQQGWIEINENEAKLTPAGADMLSEHQIEHTGLRFDRYG
                     RTGETSWRLIKFAVQVISNLATGIQDYLPAETSPFYTFQLKKWLSESQLPREILIDTA
                     YESLVQLFSEIPEEAADFLANQLSGNERTGLLPYQLAKNNDESEVYLQQSRCIHLLLA
                     QIEKRPDSLWHALIDSLLQQNFNQSMMITKQMFMNGQTIDQIMAIRHLKRGTVTDHLI
                     EWALFFDDFPYEWILSAETIERLEPNKDSVREWRFSEWNVDGQLDYGEFRLYQIYLLR
                     KEAIQNVNK"
     gene            13860..14081
                     /locus_tag="IV334_001239"
     CDS             13860..14081
                     /locus_tag="IV334_001239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HAP8943321.1"
                     /translation="MSLLCKLVPERCIACGLCQIEAPDIFDYDEEGIVLFAQEPEAHQ
                     QFVMPEEETYVKKAYQKCPVRAILLENKI"
     gene            complement(14246..14983)
                     /locus_tag="IV334_001240"
     CDS             complement(14246..14983)
                     /locus_tag="IV334_001240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase"
                     /protein_id="HAP8943322.1"
                     /translation="MKIFRRILIGLVIILGLLGGAGYYYIQQNTYEPSSEAQVLVKEG
                     KNKEDYLFFEGEKKNHGKSPLLIFYPGALVEPESYSIWAQDLAKNGFSVAIVKMPLDL
                     AVMGGNKADKVKEDFPNSDYVIGGHSLGGVMASRYVNKNHSDKHLKGVFFMASYPDKK
                     GTLTESAVPVLSITATKDKVLNQEAYQEAKQYLPERTEYVSINGGNHGGFGSYGEQKG
                     DGEAEISNADQQTVIVNTLENWLKKLK"
     gene            complement(15136..16476)
                     /locus_tag="IV334_001241"
     CDS             complement(15136..16476)
                     /locus_tag="IV334_001241"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sapep family Mn(2+)-dependent dipeptidase"
                     /protein_id="HAP8943323.1"
                     /translation="MKKFVTEQHHEQALESLSELIRIPSVLDEADSGQGHPFGKKVIE
                     ALDKVLEISEKLGFRTFKDPEGYYGYSEIGSGDELFGILCHMDVVPAGDENNWETKPF
                     DPTIKDGWLVGRGSQDDKGPSIAAMYAVKALIDAGVEFKTRIRFIFGTDEENLWRCLE
                     KYNEKEEGITQGFAPDAEFPLIYAEKGLLQAYLTGPGTNEFSVKAGGALNVVPDAAPY
                     SGEKLSEVKEALKKHEFDFEEQGEGIVVLGKSIHAKDAAQGVNAISRLAIALSEVFDF
                     GPINFLGKLVQENATGEAVVGKTEDEQSGELTMNFASLEITPEQTKIGVDMRIPVTFK
                     KDDLVAKLTKTAEKYGLTYEEFDFLDSLYVPLDSELVKNLLGTYRDITGDMTEPFVSG
                     GATFARTMNQCVAFGAMFPDTPDFMHQANERWELSSMYKAMEIYAEAVYRLCAK"
     gene            complement(16713..18236)
                     /locus_tag="IV334_001242"
     CDS             complement(16713..18236)
                     /locus_tag="IV334_001242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcC family protein"
                     /protein_id="HAP8943324.1"
                     /translation="MEQTKKKKFQMPSAYTVLFIIIAVIAVLTWFIPAGHYSVDDAGN
                     IVAGSYERVQQNPQGLWDIFMAPINGMLGVPAGELTKETPAAIPISFFILVVGGFLGI
                     INKTGALDAGIASVVKKFKGKEKMLIPVLMLLFALGGSTYGMAEETIPFYALLIPVMM
                     AVGFDTVVAVAIVLVGSQVGCLASTVNPFATGVASQAVGISMGEGIGLRFLMFVILLA
                     ISIVYVYRYASKIEKDPTKSLVYNQRKEDMKHFDIEAIGRQEVITKKQKHVNVLFFIT
                     FGIMIISLIPWTTLNANFTIFESLTNWLTNLPVLGTVLGKSMLPLGDWYFPEITMLFL
                     VMSIVVAITYGMKESEFVSEFLAGASDLIGVAIVVAVARGIQVVMNNGLITDTILHWG
                     EQGLSSLSSSVFIIITFIFYIPMSFLIPSTSGLAAATMGIMGPMGEFAGVGKDLVITA
                     YQSASGLVNLITPTSAIVMGAVAIARFDVSVWWKFTGKLIAILFVAICVILGVAAMF"
     gene            complement(18614..19225)
                     /locus_tag="IV334_001243"
     CDS             complement(18614..19225)
                     /locus_tag="IV334_001243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HAP8943325.1"
                     /translation="MKNTRVQKMVAVAMFAAIALVLQYFAFPIMPAFSFLKIDFSDIS
                     VLISMFLFGPAAGVATAFLRSVLHLLTTGFSPDNLVGDTASFLSTTIFTFPIYYFFSV
                     KKQHKGRNKFLGVITGTIALTIFMSIANYFVITPLYLMFLGLNATQMLGMSLANYVLI
                     GIVPFNLIKGFIVSAVFLVLHAQLLPWLSRKQHQFEQRHTAVK"
     regulatory      complement(19348..19473)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            complement(19555..20271)
                     /locus_tag="IV334_001244"
     CDS             complement(19555..20271)
                     /locus_tag="IV334_001244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HAP8943326.1"
                     /translation="MERLQKVIAHAGITSRRKAEEFILAGRVKVNGQVVRELGIKVGK
                     HDTVEVDEVPIYQEEYGYYLLYKPKGVISAVADDKGRKVVTDLLPMVKERIYPVGRLD
                     YDTSGILLLTNDGDFAQRLTHPKHEVDKVYVAKVKGIATKSMLAPLTKGIKIEGRKTS
                     PAKFQILSVDPKTNHSIVELTIHEGRNHQVKNMLQAVGLPVQKLKREKYGDLTLQGLR
                     PGDYRELNKKEVSSLLNHSK"
     gene            complement(20277..20846)
                     /gene="scpB"
                     /locus_tag="IV334_001245"
     CDS             complement(20277..20846)
                     /gene="scpB"
                     /locus_tag="IV334_001245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HAP8943327.1"
                     /translation="MTKLSELEALLFIVGEEGISNEELSGLLNLSEAEVTSLVKELKE
                     SYTQNSHSALHIFETESHYVLTTKKELAPLLKRYAQGTSNTLSQAAVETLAIIAYKQP
                     ITRAEIEQIRGVQVSGAVQRLAAHKLIEEKGRVEGPGRPILYGTTGYFLDYFGLKSLE
                     ELPDTQEMEISLEEDVSPDLFFDGMNKGE"
     gene            complement(20830..21627)
                     /locus_tag="IV334_001246"
     CDS             complement(20830..21627)
                     /locus_tag="IV334_001246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HAP8943328.1"
                     /translation="MASKEKEERVNELTEINIKLDVFEGPLDLLLHLIQQMEIDIYDI
                     PIATITEQYMNYIHTMKTLELAVAGEYLVMAATLMAIKSKTLLPVQEFGTDDEELWEE
                     DPREELVTQLLEYRKYKYAAERLTEKAEERSLYYTKEPMNIDGLADAEKPLKRGQVKK
                     IDLIRAMQKILERKKFSQKVEKTITPDDTTIEERMVFIEERIKRNAHGCTFDSFFESP
                     SKSEVVTTFMALLELMKKGQIIAQQKENYESIMLYPAVGDTEHDEIK"
     gene            complement(21597..21968)
                     /locus_tag="IV334_001247"
     CDS             complement(21597..21968)
                     /locus_tag="IV334_001247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HAP8943329.1"
                     /translation="MLTHYRKENRKIAMGLLSFHKKLTGKTSLLKEIDTYESEDDYEL
                     FFFTPEGSTNIQGIIGIYWATNDQLIIHDISLNPSYRGENMGFQMLDELQEKYPDVSI
                     RATEITEPYIGKWLAKKKKKE"
     gene            complement(21987..22874)
                     /gene="xerD"
                     /locus_tag="IV334_001248"
     CDS             complement(21987..22874)
                     /gene="xerD"
                     /locus_tag="IV334_001248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HAP8943330.1"
                     /translation="MKEQINDYLHYLNIERGLSANTRQSYERDLEQYLHFLQEQKIAS
                     WQDVDRFLVIRFLEKLHEEKKATATVTRMISSLRRFHQFLRQERITDHDPMQHIDTPK
                     KAQKLPSTLTLTEVEKLIEAPDTTKTLGIRDRAILEVMYATGMRVSELIGLKLGDLHL
                     SLGLVQTVGKGDKERIIPLGDYAIQWLERYLDEARPLLVKDASEMHVFVNNHGKGLSR
                     QGIWKNLKQLVREAGIYKEVTPHTLRHSFATHLLENGADLRTVQELLGHADISTTQIY
                     THITKKRMTDVYKQHFPRA"
BASE COUNT         6468 a         4926 c         3910 g         7761 t
ORIGIN      
        1 aaatcttgtc acagtccctt attctgaata aacggtgtgg aagagacagc ctatcagtag
       61 taataagaca catctctttc acagcctcat ttttgcgtaa aaatcgattt attcgaaaaa
      121 ttccccgtat acatcttcta cacgttgatc tggattgatc acgatataca agttacctgt
      181 tttttcgctg atttttgttg attgatattg attgtctaat ccttctgttc ctttatctag
      241 atatgggaag tacacaagat caggtgtgcc atttcggtaa aggatttcca ttcgctcgat
      301 atcttcgtat ttcaatgcgc gattaaacat gcccatttcc agtccgccta ggttgatatc
      361 atgtgtcgca actgcgtccc cttcatggaa gatttcgatt ttaaatccag cacacggatg
      421 gatttcaaca aattcactgc cattgattcg tccaaaactt gttgtgattt gtttgatcca
      481 aagatcgccg atatgacggc ggttgatcgt ccaggtttct ccatttctaa aataaaattt
      541 tagcgctgtc atttctttac tcattaaaaa gactccttta tgatgtaatt tattcttgta
      601 ctacctcagt ttagcacaaa tttatgtaag tggtcacatc ttatgttttt tcttctaatg
      661 acgtatctgc ccactttaag tagccaacaa agctgagcag atacagtcta gtgtttttta
      721 aatcaatttt tctttcgata aaaaatccaa aacctgtttt ttatcatttt ttattctttg
      781 ttctacgacg gctttttcag ctaattgcaa agcttcacta acccacttac caggtttttt
      841 tccagtttct tctagcaaga ttttaccatc tactgctaat tcgcttaatg acttgatagg
      901 taaacgatca taggattctc ctacggcatc catctgaggt gtttcgtcaa aatagatcat
      961 caattcttct acttgcagtg catgtttttt gcctaagaag tacaattcga atggttccaa
     1021 agctccagtt tctcgttgtc gtagtccttg gacaagtgct tgaacgtctt taatcatttg
     1081 gttagaacac ttccacgatt tcaaaaagga acgaatctca ttttctttta gatccagttg
     1141 gctgatcaat aatgcccatg cttggctctc ttctttcaat gggacggatg gtaattcagc
     1201 aaaacgtaaa agtgcttcgc cttcttcttt caaaccggga cagcaaatgt agcactctgt
     1261 ttcaataaat gcatggattc ccgcggcccc ttcatttccc aagagcatct tgatgaattc
     1321 cacatttata cgttctacag atattttacc taatagctcg tggttttctt tgatggcgta
     1381 gaaggtttct tcctctaatt tgaaccctag ttgactgaca aaacgcaacc cgcgcatcat
     1441 tcgtaaggca tcttcatgga accgctcatg aggattgcct acagctcgca agatctgttc
     1501 ttctagatct tgcagaccat cgaacaaatc gatgatttct ccatcttctt tcaaagcaaa
     1561 cgcattgatc gtaaaatctc ttcttttcaa atcttctgcc aacgaacgaa cgaattcgac
     1621 atgatctggt ctacggtagt cctgataagt tgattccgtc cgaaatgtgg tgatctcata
     1681 ttgttcatca ttttctaata ccaacacagt tccatgttcg atcccaatat ctatcgtttt
     1741 tggaaaaagc tgtttgattt cttctggaaa tgcgcttgtt gcgatatcca catcatggat
     1801 tggctggttc agcaagatat cacgaacact tccacctaca aaataagctt catatcctgc
     1861 attctctaat tttttcagaa ctggaattgc cttttggtat tccagaggta attgttttaa
     1921 tttcattttt ccaacctttc ctgcgtgttt cttgtcctga gataaagact cagttgcatg
     1981 gtgactgttt gacaagtccg tttcagagtc ttcaaggcca ggaccacttc atcttgttta
     2041 ctcgtcctta cgctcaaagc gattatggtc tctttgattg aatttttcca tgtttttttc
     2101 aaaaacttca gttaagtcga tattcaatga attagccatg atcatcgtaa cgaataaaac
     2161 atcaccaagt tcttctgcta ccgagttaac tggttcagaa gcctttttgc ttttttctcc
     2221 ataagtgtgg ttgacttctc ttgccaattc acctacttct tcagttaatc gagccatctg
     2281 agctaatgga gagaaatacc ccaccttgaa ttgctggata tattcatcca cttcctgctg
     2341 catactgtat aaagaacgct ttttatccat ctttttccct ccaaaactat cttaccaaaa
     2401 ctttaacgca aatcctagat aagagcaatt gagaaaatcg aatttccatg ctacatttag
     2461 gaaggaattt ctaaatggaa attctgtgaa aatacataat tgaaagctgg aattcatatg
     2521 gtcaaacgaa aaacagtaaa agaagttctt tttatcatta taggaacaag tatttatgca
     2581 tttggtcttg tttacttgaa catcgctaat catttagcag aagggggagt ctcagggatc
     2641 accttgatct taagagcttt atttggtatt gatccagctt atacgacttt attaatcaac
     2701 atcccattga ttttactagg aggaaagatc ctcggtaaac gttccttggc ctatactgtt
     2761 ctaggtacac tttctttgtc attcttttta tggatctggc aaagggttcc actagaaatc
     2821 aatttacagc atgatctact gatcgtttct ctacttgcgg gtttagttgc aggtgtagga
     2881 agcgggatcg tctaccgcat gggtggaaca acaggcggca gtgatattat cgctcggatt
     2941 ttagaacaaa attacggcat cactatgggg cgttccctat tggcatttga tatcatcgtc
     3001 ttacttgctt cattaaccta tatcgacttg aaacgtatga tgtatacgtt gattgtttcg
     3061 tatgttttca gtcgtgtgat tgattcgatc ttagatggcg gatattctgc aaaaggtcta
     3121 cttgtcgtct ccaacaaaag cgaagagatt gcaccactac tgatgaccgg tctgcaaaga
     3181 ggtgtgactt tcctttctgg agaaggcgca ttctctggta attcaaaaaa aatcatctat
     3241 atggtcgtta gctctagtga attaactgaa gctaagagga tcgtccacga gattgacgaa
     3301 aaagctttcc tttcaatcat caatgtgcat gaagtggaag ggcaaggctt tacttatttg
     3361 aagccacaga caagattact gaaaaaacta taagaaaaaa gacaaagata ggctatgagg
     3421 aaagattcca catccagctg acctttgtct tttttagtac tatactattt ttaactaatt
     3481 gttatgttca cttacccggt tacttttctt ttgtgtctgc aaaaggatct ttcggttaac
     3541 ttcatcagat aatttcaaaa aagcatccct atctccatct tctagtgctt gatcgatttg
     3601 agcataaagc atttttagtt ttgcctcttc ttctttttct tgaaaatatc gttcaattaa
     3661 ttcagtatct tcttcactga ctgaagaatc ccaagaagaa aatggattgt cttctaagat
     3721 agatagatat tctcttgtct gccaagcatt ttcgaatata cattccacat ataaaggctt
     3781 tttccaattc aaacggattt catgaaaaat ctggtctgtg tccgtaaatt cttttccctg
     3841 caaaaacaat ttcatgggtt ccccttcaac agatagatca ctgatctgca gtcctcgatc
     3901 cgcaaaattc gccccttcaa caaagtggac attgcttaaa atcgcttcat gattggcaag
     3961 ataattcaag atccaaaaaa cttctcttcg gctaaaagat acatgattga ccatccacgt
     4021 taaaaaattc ttcttttccc gtacatcaat tagcattttt ctcacctctc tgttaggtca
     4081 tctaataatg acagtgcctc catatctcct ggttcaagtg ttaaataata agtcaataat
     4141 tcttgcgctc ttttcagctg cccttcttct cttaagaaaa aggcatactc tttcaagaaa
     4201 tctggttcat gtgatagttc atgataagcc tgttcatagt gtacagctgc aacggtaaag
     4261 tcttctaatt cattataagc atgggctagg ttccattctg cataaggatt agcagtttct
     4321 tccatctgat tgatcacttg tatcacatct tcatatcttt catcatccaa gtaaagatta
     4381 cttaaagtaa gtaatgtttc atcttgtttg tctcctactt ctaaagcttt cagtaaaagt
     4441 tcttctgctc gctgtctatc atgcagacgg aaagcatttt ccgaggccag gtgataaagt
     4501 tcgacttggt aaggattttc tttgattcct tcttctaaca ctgtttgtgc ttcttcaatc
     4561 atttcttctt cttgaagagc ttgtcctaga tatagatata acgattgata atgcggatta
     4621 acttctcgaa gttcttccag atagttgatt gcctgctgat tttctttcaa ttgaagattc
     4681 gtaaaagcta gctggaacag tcgatcatct gtcctttctt ctttcagtgc ttcttctaaa
     4741 ggtacgatag ctgcttcgaa ttctccttgc atacttaagg attgtcctaa acgttcctgc
     4801 atcgagattc ctgaaatttc atttatcccg cttgccaaca atgtttcata aaccactgct
     4861 gcctctttga aacgatcaac tgaaaaatac aattcagcta gcgcaaactg gatcaatggt
     4921 tcatcaggaa gcaacccagc tgcttctttc aattttgctt cactcacttc agggattccg
     4981 ataacttggt acaaatcagc tatcgccaat aagctttgcg gatagtaatc actttgctta
     5041 tccacttttt ccaggtattc aaaagctaag tcgatctcat tatcttcgat tgcaatttct
     5101 gctaacggga tgttcagtcc gtcattttct gggtattggt tgatcagttg ttcaaaaatc
     5161 tgttttgctt cgatcaaaaa gccaatagcc agcaattctt cgcctagttc agctaatata
     5221 tctgctgggt cttcttttaa tgctttttct aaaaagattt gtgcttgtga caagtcttca
     5281 tctgataaag cttgcaacat tttttcgcta tttgtattca tttcaattcc ctcttatctc
     5341 actgacgatt tgttgaaata ccgtcattgt tctcaataat tctttttcgt ttggatgttg
     5401 gtatccgtct attataccta ttttttctaa aacaagtatt tttttgcttt tttctgccaa
     5461 aaaaagaact ttttccaaat actccatctt tgaaatctgt tccagccagt ggattggata
     5521 gccaagcaag tccagccaca caaaaaagtt ttttagttgg aaatcaaaag gatatgcttg
     5581 gagattccac acgaaatgaa acatcattcc taaaaatttt ttcatactag cagaaagtag
     5641 atggccattt tcggtcagat aaaaagcttg ttcaaacagt ttaccataat ttgacaggtt
     5701 ttctgcctct aattgataaa aatagatcat ttcttcgatc atcccggcaa acggccgagt
     5761 cgttaattgc tttttatttg gatagttttt atacagattt tgcaaaaaac gatgatcaca
     5821 aatcattccg caacagataa aagtcaaaaa gtcaatctgc tttccgtcca cctgccgctc
     5881 gctgattgtt tgatcataaa cgatgcgttc tggcagattg atggtttgca gcactacacg
     5941 atttggtagc tgcaaaatcg tccgctgcgg tgttaatgct cggcttagag aacggatcga
     6001 tacagggatc atccatagtg atgaatcaag ggttgtatgt ttttgaaaaa aacctgccag
     6061 atccatgatc ccttcatttc caaatccgat gataaacatg gattgatttt cgggatagcg
     6121 tttgacgaaa tcaagcaggt tctttaattc tgttaaatga ttgcattgag tgttggcaca
     6181 aatataccag tcattgtttg cttgatttgc aaataacggg ttgatcttat ctgcaaacaa
     6241 atcataatag cgctgattcg ttaaaaataa gattggctga tcaatggaaa gttctcttag
     6301 ctgactgact agcgtttctc cgtaaaacac tttcgtcaac gcatcttcta cttgataatc
     6361 aagttccata cgcatccctc ctcttcttat ttttatttta ccattagtaa gcaaattcta
     6421 gaaaagataa gagcaaagct cacctcataa cttggaaata aataaaaatt aaataaaata
     6481 catgtgtttt ctatgtatga catacggatt cggaatctct gtctcttatt tattcctttt
     6541 ctgctaggaa ataaaaagaa aagcacttgt agccctagag ccacaagcgc tttttcaact
     6601 gtcttattta acagcgtctt ttaatgcttt acctggtttg aatgcaggta ctttgctagc
     6661 tggaatttcg atttcttcac cagtttgtgg gttacgacct ttgcgagctg cgcgttcgcg
     6721 aacttcaaag ttaccaaaac cgattaattg aactttttcg ccgttagcta aagcatcttg
     6781 aatagttgaa aatacagcat ctactgctgc agttgcatct tttttagtta aatcagtagc
     6841 tgaagcaact ttttcgatta attctgcttt gtttgccatg gatagattca cctcctccac
     6901 aagagatatg atagaaatat catttagtta aaactttctg agtggtccag aagtttaaaa
     6961 agaaatattg attaacttca atattctgtt atcacgatat catatgaacc gcttaatagc
     7021 aaggttttta ccaattttca catgcttttt ttagtcatag aaacgtaaca aacgaaactg
     7081 tctttttctt ttgaaaaaaa aacgtcgata tggtaaaatg cctattttct cctacgaggg
     7141 ataattcgga tggacgtccc ttcaaaagta aatgcttttc tgatttgatt ttctaagaaa
     7201 cgttcataag aaaaatgcat caattcctct tcattgacaa aaacgacaaa tgtaggtggt
     7261 ttgacagcga cttgtgtagc ataaaaaatc ttcaggcgtt ttcctttatc tgttggtgtt
     7321 ggattgattg ctacagcatc cataatgata tcattcaaca cagccgaaga gatacgaaga
     7381 ttctggttca tacttacttg ttcgatcatt tcaggcagac gttccaaccg ttgtttagtc
     7441 actgcagaaa caaaaacgat tggagcgtaa tcaagatacc ggaattcatc acgaatctct
     7501 tgttcgaaat cacgcattgt attggtttct ttctttacaa gatcccattt attcacgacg
     7561 ataataattc cacgaccagc ttcatgggcg taacccgcta ctcgcttgtc ttgttcccga
     7621 atcccttctt cagcattcag taccatcaaa acgatatcag aacggtcaat ggcacgcatt
     7681 gcacgcatca cgctgtattt ttcagtattt tcgtatactt ttccgcgttt acgcatacca
     7741 gctgtatcta tcattaagaa tttctgacca ctttcagaaa cgaagtgggt gtcgattgcg
     7801 tctcgggtcg ttccttcaat atccgaaaca attacacgtt cttctcctaa aatcgcattg
     7861 atcaaagagg atttaccaac atttgggcga ccaatcaaac taaacttaat cgtgtcgtcg
     7921 tcctcttctt cagtctcttc agagaaatac ttcactgctt catctaaaat atcaccaatc
     7981 cctagtccat ggcttcctga aacaggataa ggatcgccta gccctaagga ataaaattca
     8041 tagatatcgt ttctcatttc tggattatct actttattga ctgctaaaat cactggttta
     8101 ttacttctgt ataaaatttt cgcaaccatc tcatctgcgt ctgtcacgcc ttcgcgtccg
     8161 ctgactacac aaatgattac atctgcttct tcaatcgcga tttctgcttg atgcttgatc
     8221 tgatccataa aaggttcatc gccaagatca attccgcctg tgtcgatcac gctgaattct
     8281 cgacctaacc attcaccttt tgcataaata cgatcacgag ttactccagg agtatcttcg
     8341 acaatcgaga tacgctcacc agcgatgcgg ttaaaaatcg tagacttacc gacgttcggg
     8401 cggccaacga ttgcaattgt tggatttgcc atattatttc ctcctcacaa gtttccaatc
     8461 tcacttagtt taacgaatgc attgccaact ttcaagcata gattgtcaat taaacgaagt
     8521 atttttattc cttattgaaa tactaatcgc ttttcaaaag aaaaactgag acaaaagcag
     8581 ctgctttcat ctcagtcaca gatacaaaat caattatttt tcagaatcat cggttgcttt
     8641 ttcttctact tcttctttca acgcatcacc taaaatgtct cccatagtaa atccagtatt
     8701 ttcttctggt aattcataag actcaacatt ttttggttct tcttctgaag ctggtttttc
     8761 ttctaaagct ttgatactca atgcaatacg atgttcttct ggatggatct ctaatacttt
     8821 cactttcact tcgtcacctt catgaagcac ttcatgtggt gtcgcaatat gtttatgtga
     8881 aatttgagag atatggacta gcccttctac tccagggaac acttcaacaa aggcaccgaa
     8941 gcttgtcaaa cgtttaactg ttccgtctaa tactgtgcca actggagctt ttgtatcgat
     9001 ttctgaccaa ggtccaggct gtgtgtcttt gattgacaag gaaacacgtt cttgttcagg
     9061 attgatagac aacactttga catttacatc atcattgaca gccaatacat cacttggttt
     9121 tgctacatga ctatgtgaaa tctctgagac atggactaga ccgtcaattc cgcctaggtc
     9181 gacaaatgcg ccaaaatcag taagacgtgc aacacgtcca ctgagaacat ctccctcatg
     9241 gatagttgaa agaatttctt ttttcttttc ttctctttcg ttttccacta ccgctttatg
     9301 cgataagatc aaacgatttt ctgatggttc gatttcgatg attttaaagc tcaatgtttt
     9361 gcctttatag tctgaaaagt ctgcaacaaa atgatcttcc accattgatg cagggacaaa
     9421 accacgtaca ccgacgtcaa cgactaaacc gcctttgaca acatttgtaa caggtgcttc
     9481 gatgaccttg ccctcttgga aatctctttc gatatcttcc caaacttttt tagcatctaa
     9541 acggcgtttt gaaagtaggt agctgccgtt ttctttatct ttgccgatcg tactgataac
     9601 gactaaatct aatacatcac cgacttttac tacttcgctg atatcttcta ccggcaaagt
     9661 tgataattct tttgccggaa cgactccttc aactccagcc ccttcgattc cgacaatcac
     9721 ttgtttgtct tcaatgacta aaacttcgcc cttaacaaca tcgccaactt ttacttcttg
     9781 tacgcttttc attgcgtctt ccattgactg gttgttctct tgattttgat caaattctgt
     9841 cataacactt gtcctcctaa ccaacattct gccgtaagta cggtactgtt tttagtttaa
     9901 ttgattattc aaaaaaaata aagcgatttc gttcgttttc cttgaaaaaa caattgtttt
     9961 tcattttttt tcttaattta aaaaagaaga taaccttttt tcaaaacaac agcttttatt
    10021 gctgcaacga cttctggaat cgatttgcca gtcgtatcga tacgaacagc atcctctgct
    10081 tgcttcagcg gagagacgtc ccttgttgaa tcataatggt ctctttcctg tatctctttc
    10141 gtcagtgttt caagatcagt cggaattcct ttttcttggt tctctttgaa acgtctttct
    10201 gcacgctcac ttacgcttgc tactaaaaag attttcactt ctgcattcgg taatacagct
    10261 gtcccgatgt ctcttccgtc catgacaacg ccaccgcgtt cccctatttt tcgttggaca
    10321 gcaactaatt cttctctgac ttttccatgt gcagagactt cagaaacagc ttttgtgaca
    10381 tctggttgac ggatttctaa agtcacatct tttgatgcta tgaatacatg ctgtccatct
    10441 tcttgttgcg aaaaagagat aggatacttt ttgatcagcg aaactagccc ttcttcatct
    10501 gaaaaagata cattgttcgt aatagctaaa taagttactg cgcgatacat ggcaccggta
    10561 tctgtataaa tataattcaa ttcttttgct aatatcttgg caacggtact tttcccagat
    10621 gaagcaggcc cgtcgattgc aatactgatt tgtgtcatga tataactcct ttttctcaga
    10681 aaaaaagccg caaagcgcgg ctattattat ttaattctta attcttgacc agggtaaagc
    10741 ataaagttgt ttggatctaa gccattcaat tcaaacaatt gatcagtggt gatccctgca
    10801 cgctcagcca cttgttttgg accttcgcct gcttgaacag tcgttgttga accggctgca
    10861 gctgatgaag aaggtgttgt ttctgctgca ttgctgcttg aatcccctgc ttgataagtt
    10921 gcctcacttg aggaagtcgg tgttgtctct gctgcaatac tgctttctga cgttacacct
    10981 gtatcttgag acacagtaga ttcttccgcg attgtactag attctactgt tgaagtctca
    11041 gttgactcct cttttgttgt acttgtagaa ctttctacta ctgatgtcga agtttcagaa
    11101 gaagttgctg ctgttgcatt gttgcttgat ccatttctga tccagaaata agcacctgct
    11161 ggaatagcga tacataagac cagtaaaatc aaaaatagcg aaagaaataa tgtatttcct
    11221 ttcttttgtt gtctctgaga acttcttgat gaaacttcat catctgtatc gtagattggt
    11281 tgttcccatg gttcttgtgt ttcgttattt tctgtagatt gatgtctatc ttttctgctc
    11341 actgttttac ctcctaaact ctatcgtgtg tattatacca taggaaaaag tgtcttgtcc
    11401 taaaaaatca atgttccatg ttttttttaa ataaatttag taaaatcgtt tcccaatgaa
    11461 gttgttcagt cttagaaaaa agcttcctct ttggcagaag ttctgctttt gctccatcaa
    11521 tatcacaaca attctgcggc ttttcggaga gtttttcgcc aaaataagct aagatgaatt
    11581 ttctgcggca gttttcttca ttaatatagc gaagcatctg ctgcaggcgg aattcttttt
    11641 ctttttcctg acgtttgagc cgttccaccc aattttctgg tttttcagac tgctggatca
    11701 gctccatcca ttttttttgc agctcatcaa agggagcttg ctttgtggca tattccaaat
    11761 aaagctcaaa actttgtcgc tgttctctag aaagttgatt aaagaaataa tggatacgtt
    11821 catccccact ttgataaaaa agaattgctg cactttcttc ctgatctctg cctgctcgac
    11881 cgatttcttg tacatagttt tctagactgt ctggcaaatc ataatggacc acataacgga
    11941 tatcagattt atcgatcccc ataccaaatg cattcgtagc aatgagaaac tgcagctgat
    12001 tttttacaaa ctgctgttga agttgtcttc tttgagctgc atcaagacca ccgtgatagt
    12061 atccaactgt aaaacgttcc ctgaataaat gatacaatcg ttcgacttct ttttttgttg
    12121 cacaataaat gattgccgct ccttgtgctc tttccatgaa gtgttcaagt tcttgttctt
    12181 tttgctgcgt tttccgaaca aaaagcgaga tattttgccg attgactgat tgtctgatct
    12241 cttttggtgg tcgatcaaat agtacatgac gtatatctct tgctactaaa tctgttgctg
    12301 tcgccgttag tgccagtgtg accgggtttc ctaaaagctt tcggattttt cctaactgtt
    12361 gatattctgg acggaaatca actccccatt gagacacgca atgagcctca tctactacat
    12421 ataacgcaat attttgtttt ttcagatgct ctaaaagaga aggattagtc aacatttcgg
    12481 gactcaaaaa caaaaattta tattgcgata gatgacggag gacgtatctt ctttcagatt
    12541 gcgaaagtaa actattgaaa gcagctactc gtttctcccc tcgtttttgt aattgcgtca
    12601 cttgatcttc cattagagaa agcagcggtg acacgatgat gaccagacct tccattaaat
    12661 accccgtcaa ttgatagcaa aggctttttc ccgttcctgt aggcaaaata gcgagagtat
    12721 cttctccatc aagcaacgcc tgaataattt cttcttggcc gggacgaaat gaatcaaatc
    12781 caaaatattt ttttaattca tttattaaca ttctgtatcg cttcctttct caatagatag
    12841 atttgataca aacgaaattc tccataatct aattgtccat ctacattcca ttcgctaaag
    12901 cgccactctc gcacggaatc cttatttggt tccaaacgtt cgattgtttc tgcagagaga
    12961 atccactcgt atggaaaatc atcaaaaaac aaggcccact caataagatg atctgttact
    13021 gttcctcttt ttaaatgacg gattgccatg atctggtcaa ttgtctgacc attcataaac
    13081 atctgcttgg tgatcatcat gctttgattg aaattttgct gtaataatga atcgatcaaa
    13141 gcgtgccaaa gcgaatctgg ccgtttttcg atttgtgcta acaaaaggtg gatacatctg
    13201 ctttgttgca gataaacctc tgactcatca ttgttttttg ccagctgata aggaagcagc
    13261 cctgttcgct catttccaga taattggtta gctaaaaaat ctgctgcttc ttctggtatc
    13321 tccgaaaaca gctgcaccaa actctcatac gcagtatcaa tcaaaatttc acgtggcaat
    13381 tggctttcac taagccattt ttttagttgg aaagtataaa aaggacttgt ctctgctgga
    13441 agatagtctt gaatacccgt tgccagatta gaaattactt gaacagcaaa tttaatgagc
    13501 cgccaactag tttcccctgt tctgccatag cggtcaaaac gcaaaccagt atgttctatt
    13561 tgatgttcgg acaacatatc agcccctgca ggcgttagct ttgcttcatt ttcatttatt
    13621 tcgatccaac cttgctgcgc aattttttgc aaaatttgat aaaacttatc ctgcttcaat
    13681 tctggaaaac tgttatggat aaacaaaagt tcgtgggtaa atccataaat aagtactgaa
    13741 gtcgttcgtt ttcccacaag gagttggtaa agagaactaa tccgcagctt gtgtcctgat
    13801 gaaaatagcg ataaaataaa aacaatcata ttattctcct taagttagga tggttagaaa
    13861 tgtcactact atgtaaactt gtacctgaac gctgtattgc atgcggtctt tgtcaaatcg
    13921 aagcgccaga tattttcgat tatgatgaag aaggaatcgt tttatttgcc caagaaccag
    13981 aggctcatca acagttcgtg atgccggaag aagaaacata tgtaaaaaaa gcgtatcaaa
    14041 aatgtccagt tcgagcgatt ttattagaaa ataaaatata aatataaacg aaactttaaa
    14101 aacgtacact aagttctcta aacttgaaaa taagccggga tctgtttctc ttgttccgtg
    14161 aaatcccgac ttattttgaa agattcattt gctatctata gttaattgac tagttttttc
    14221 acggacccta taggacccat ttatattatt tcaacttttt cagccagttt tccaatgtat
    14281 tgacgatcac agtttgctgg tctgcgttag aaatttctgc ctctccatct cctttttgct
    14341 caccatagct gccaaaacct ccatgatttc cgccattgat tgatacatac tctgttcttt
    14401 ccggtaaata ctgcttagct tcttgataag cttcctgatt caatacttta tcttttgtcg
    14461 ccgtgatcga taaaacagga acagctgatt ctgtcaatgt tccttttttg tcgggataac
    14521 ttgccatgaa gaatacccct tttaaatgtt tgtctgaatg attcttattg acataacgac
    14581 ttgccattac gccacctaat gaatggccac cgatcacata atcggaattt ggaaaatctt
    14641 ctttgacctt atccgctttg tttccgccca tcactgctag atcaagtggc attttgacaa
    14701 tagcgactga aaagccgttt tttgctaaat cttgcgccca aatactataa ctttctggct
    14761 ctacgagagc accaggataa aaaataagta atggagactt tccatgattc tttttctctc
    14821 cttcaaaaaa taagtaatct tctttgtttt ttccttcttt taccaacact tgcgcttctg
    14881 acgatggttc gtacgtattt tgctgtatat agtaataacc ggctccaccg agtaatccta
    14941 aaatgatgac taagccaatt agtattcgtc taaatatttt cactttctcc caccttttct
    15001 atctgagtga cccctttttc ttaatcaagt atgattatac cttaaaaaaa taagagagaa
    15061 atgcaaactt tccagcatca aataaaaaaa gacggaatat ccatttgaat atccgccctt
    15121 tttgtatgtt tatttttatt tagcacataa acgatacaca gcttctgcat aaatttccat
    15181 tgctttatac atactactta attcccatcg ttcattcgcc tggtgcataa agtcaggagt
    15241 atcagggaac atcgcaccga atgccacaca ctggttcatc gtacgagcaa aagtagcgcc
    15301 gcctgataca aacggttcag tcatgtctcc agtaatatcg cgatatgtcc ccaatagatt
    15361 tttcactaac tcactgtcta gtggcacgta taatgaatca aggaagtcaa attcttcata
    15421 agtcaaccca tatttttctg ctgttttagt taatttagct actaaatcat ctttcttgaa
    15481 tgtaacaggg attctcatat ctacaccgat ttttgtttgt tctggtgtga tttccaaact
    15541 tgcaaagttc atcgttagct cgccggattg ttcatcttct gttttgccca cgacagcttc
    15601 accagttgca ttttcttgaa ctaattttcc taagaaattg attgggccaa aatcaaatac
    15661 ttctgaaaga gcaattgcca gacgagaaat agcattcact ccttgtgctg catctttcgc
    15721 gtggatactt ttaccaagaa cgacgatgcc ttcaccttgt tcttcgaaat cgaattcgtg
    15781 ttttttcaat gcttctttta cttcagaaag cttttctcca gagtaaggag ctgcatcagg
    15841 aacaacattc aacgctcctc cagcttttac agaaaactcg ttcgttcctg gtcctgtcaa
    15901 gtaagcttgc aataagcctt tttctgcata aattaaagga aattcagcat ctggcgcaaa
    15961 tccttgcgta attccttctt ctttttcgtt atatttttct aaacaacgcc ataaattttc
    16021 ttcatctgta ccaaagataa aacgaatacg tgtcttgaat tccacgccag catcaatcaa
    16081 agctttgacg gcatacattg ccgcaataga aggacctttg tcatcttggg atccgcgacc
    16141 aactaaccag ccgtctttta tagttggatc gaatggtttt gtttcccaat tattttcgtc
    16201 ccctgctgga acaacgtcca tatgacacaa gataccgaat aattcatctc cagagccaat
    16261 ctctgaataa ccgtaatagc cttctggatc tttaaatgtt ctaaatccta gcttttcact
    16321 gatttccaat actttatcca aagcttcaat cactttttta ccgaagggat gtccttgtcc
    16381 tgaatctgct tcatctagta cggaaggaat tcggatcaat tcgctcaatg attctaatgc
    16441 ttgttcatga tgttgttctg tcacaaattt tttcatgata ttatcctcct aatatgttaa
    16501 aaagggtcat gtcaaattcc tccgacacaa cctcttcttc tcaaataatg acgttttaag
    16561 cctgctccat cagcaagcag aatgttctta acaaatgatg aaagaaaaaa cactcactga
    16621 atggagctat tgacttgtcc acgtgcttat cgatagccgt ttatcggttt cttcattctt
    16681 atgatagacc ttgtacctgt atttgtctag gtttagaaca ttgctgctac acctaggatc
    16741 acacagatgg ctacaaataa tatcgcaatt aatttaccgg taaatttcca ccacactgaa
    16801 acatcgaaac gagcaattgc tacagcaccc attacgatag cagatgttgg tgtaattaag
    16861 tttactaaac cagatgcaga ttgataagca gtgattacta aatccttacc gacgccggca
    16921 aattcaccca taggtcccat gattcccatt gttgcagcag ctaagccgga agttgaagga
    16981 atcaagaatg acatagggat atagaaaata aatgtaataa tgatgaatac acttgaagat
    17041 aatgaactta atccttgttc gccccaatgt aagatggtgt ctgtgattaa tccattgttc
    17101 ataaccactt ggataccacg agcaacagct actacgattg ctacaccgat caaatcagaa
    17161 gcaccagcta aaaattcaga gacaaattca gactctttca tgccataagt gattgctact
    17221 acgatggaca ttactaagaa taacatagta atttctggga agtaccagtc acctaatgga
    17281 agcatgcttt tacctagaac cgtacctagt accggaaggt ttgttaacca gtttgtcaat
    17341 gattcaaaaa tcgtgaaatt cgcattcaag gttgtccaag gaatcaaact gatgatcatg
    17401 ataccaaaag taataaagaa caatacatta acatgttttt gttttttcgt gattacttct
    17461 tgacgaccga ttgcttcaat atcgaaatgc ttcatatctt ctttacgttg gttgtatact
    17521 aatgattttg ttggatcttt ttcgatcttt gatgcataac gatacacata tacaatagaa
    17581 atagctaaca aaataacgaa catcaagaat cgaagaccaa taccttcacc catcgaaatc
    17641 ccaacagctt gtgaagctac gcctgttgca aaaggattta ccgttgaagc tagacagcct
    17701 acttgagacc ctactaatac gattgctaca gctactactg tatcaaagcc tacagccatc
    17761 atgactggga tcagtaaagc atagaatgga atcgtttctt ctgccattcc gtaagtagag
    17821 ccgcctaatg caaataacag catcaatact ggaataagca ttttttcttt tcctttgaat
    17881 ttcttcacaa ctgaagcaat cccggcatct agtgcccctg ttttgttgat aatacctaaa
    17941 aatccaccaa caaccaaaat gaagaaagag attgggattg cagcaggtgt ttctttagtt
    18001 agctcccccg ctggtacacc tagcatccca ttgattggcg ccatgaaaat atcccacagc
    18061 ccttgaggat tttgttgtac tcgttcatat gatccagcga cgatgttgcc tgcatcgtct
    18121 acactatagt gcccagctgg tataaaccaa gtaagtacag cgattactgc aataataata
    18181 aataagaccg tatacgctga aggcatttga aattttttct tttttgtttg ttccatcttc
    18241 ttcttccttt cttttccgtt agagacaaca attttggctc taatgttatt ttatcaaaaa
    18301 gagtatcaga acaaaaataa taacacgcat tttgtaaaac ttttgttcaa aaagttcgtt
    18361 tttccctatt ccctcctctt ttttcagttt tttaaagctt tcttcttttt cattgtagcg
    18421 tttccttttt tttataaaca aacattttcg tcttttaaaa ggagaagaat ttcacatttg
    18481 caaagaattg attgattttt atgcataaat caaaaaaata ctcaaaacaa acaaataaaa
    18541 cgtttacatt attgcatgtc ttgtattttt ttccaaaaaa aaagaccaag aaatttcttg
    18601 atctttttga tatttatttg actgctgtat gacgttgttc gaactgatgt tgttttcgac
    18661 ttaaccatgg aagaagttgc gcatgcagca ctaaaaagac agcactcacg atgaatcctt
    18721 tgatcaagtt gaaaggaacg atcccaatca gtacatagtt tgctaaagac atgcccagca
    18781 tctgtgttgc gttcagccct aaaaacatta agtaaagagg cgtaataacg aaataatttg
    18841 cgatactcat aaaaatagtt agagcgatgg ttcctgtaat gacacctaaa aatttgtttc
    18901 ttcctttatg ctgtttcttt acactaaaga aataataaat cgggaatgta aagatcgttg
    18961 ttgataagaa actagcagtg tctcctacca aattatccgg agaaaaacct gtagtcagca
    19021 gatgtaatac tgaacgcaag aacgctgttg caactccggc cgctggaccg aataagaaca
    19081 tgctgatcaa taccgagata tcactaaaat cgatcttcaa aaaactaaaa gccggcatga
    19141 tcggaaaagc aaaatactgt aaaactaatg cgatcgcagc aaacatcgct actgcaacca
    19201 ttttctgtac acgggtgttc ttcatttgaa atcctcctgt taggactcct cttcagcgaa
    19261 ctttttgacg tttctctcca aatgaaaaaa ctctattttc cagggaaaat agagttagag
    19321 ttatttgatc aagcgtcgtc aaataagctc tatcttcttt atccagacta tactgtcggt
    19381 atcggaattt caccgattca gctatttttt tgcaaaatag gtcgtggact ataaccaccg
    19441 gtcgggaatt tcaccctgcc cctgaagacg aaccttatat aatttttgtt tacatattca
    19501 ttatacatat ctattcaaaa aacgcaacat ctaacaatta taaaaagtaa gtgttcattt
    19561 tgagtggttc agcaaactgc tgacttcttt tttgttcagt tcacggtaat ccccaggacg
    19621 taggccttgt aaagtcagat ctccgtattt ttcacgtttt agtttttgca caggaagacc
    19681 aactgcttga agcatatttt tcacttgatg attacgtcct tcgtggatcg tcaattccac
    19741 gatactgtgg tttgtcttag gatccactga taaaatttgg aatttagctg gagatgtttt
    19801 tcgtccttcg atctttattc cttttgtcaa aggtgcaagc atcgattttg tagcgatccc
    19861 ttttaccttt gcaacgtaaa ctttatccac ttcatgtttt ggatgtgtca ggcgctgagc
    19921 aaaatctcca tcatttgtca gtagcaagat ccccgaagta tcgtaatcca gtcgacctac
    19981 aggatagata cgttctttta ccatcggcaa aagatccgta acaacttttc ttcctttgtc
    20041 atctgcaaca gcagaaataa ctcctttagg tttgtacaat aaatagtaac cgtattcttc
    20101 ttgataaatt gggacttcat ccacttccac agtgtcatgt ttcccaactt ttatccctaa
    20161 ttcccttaca acttgtccgt ttacttttac acgtcctgca aggatgaatt cttctgcttt
    20221 tcgacgagaa gtgatgccag cgtgggcaat tactttctgc aatctctcca ttacaattat
    20281 tctcctttat tcattccatc aaagaataga tctggtgaca cgtcttcttc aagtgaaatt
    20341 tccatttctt gtgtatcagg cagttcttct aatgatttca aaccaaaata atctaagaag
    20401 tagccagttg ttccatataa tatcggccga cctggtcctt ctactctacc tttttcttca
    20461 atcagtttat gggcagctag acgctgtacc gctcctgaaa cctgaactcc tcgaatctgc
    20521 tcgatttcag ctcttgtgat tggctgttta taagcgatga ttgctaaggt ttcgacggcg
    20581 gcttgagata atgtgtttga tgtcccttgc gcatatcgct taagtaaggg agccagctct
    20641 tttttagtcg ttaatacata atggctttcc gtttcaaaaa tatgcaaagc tgagtgcgag
    20701 ttttgagtgt aagattcttt taattctttc actaaacttg tgacttctgc ttcgctgaga
    20761 tttaacagtc cgcttaattc ttcattactg atcccttcct cgccaacaat aaaaagaagg
    20821 gcttcaagtt cacttaattt cgtcatgttc ggtatctcct actgctggat aaagcataat
    20881 tgattcatag ttttcttttt gttgcgcaat gatctgccct tttttcatca attctagcaa
    20941 agccataaaa gtagtgacaa cttcactttt agaaggtgac tcaaaaaaag aatcgaaagt
    21001 acagccatgc gcatttcttt ttatacgctc ttcgataaat accattcgtt cttcgatcgt
    21061 tgtgtcatcg ggcgtgatcg ttttttccac tttttgagaa aatttcttac gttctaagat
    21121 tttctgcatg gcacggatca aatcgatttt tttgacttgt cctcgcttca acggcttttc
    21181 agcatccgct aacccatcga tgttcatagg ttcttttgtg taataaagac ttcgttcctc
    21241 tgctttttct gtcagtcttt cagctgcata tttatattta cggtattcta aaagctgagt
    21301 gaccaattct tctcgaggat cttcttccca taattcttca tcgtctgtgc cgaattcttg
    21361 gacaggaagc aatgtcttgc ttttgattgc catcaatgta gcagccatca ctaagtattc
    21421 tcctgcaaca gctagctcga gtgttttcat tgtatggata taattcatat attgctccgt
    21481 gatcgtagcg atcggtatat catatatatc gatttccatt tgctgaatca gatgaagcag
    21541 cagatcgaga ggaccctcga atacatctaa tttaatattg atctccgtca actcattcac
    21601 tctttcttct ttttctttgc tagccatttt ccaatgtaag gctctgtgat ttcagttgca
    21661 cggatggaga catccgggta cttttcctgt aattcatcta acatttgaaa gcccatattc
    21721 tcaccgcgat aagacggatt caaagaaatg tcatggatga tcagctgatc gttcgttgcc
    21781 cagtaaatac caataatccc ttgaatgttg gttgaacctt ctggcgtaaa gaaaaaaagt
    21841 tcataatcgt cttcagattc gtacgtatca atctccttca acaaagaagt tttacctgtc
    21901 agtttcttat ggaaactcaa tagtcccata gcaattttac ggttttcttt ccggtaatgt
    21961 gttaacatct tctcaccttc ctatgactaa gcccttggaa aatgctgctt ataaacatcc
    22021 gtcattcttt tcttcgtgat atgtgtataa atctgagtcg tagagatatc cgcgtgacct
    22081 aacaattcct gtactgtccg caaatcagct ccattttcta gcagatgtgt ggcaaagcta
    22141 tgccgcagcg tatgtggtgt tacttctttg taaatcccag cctctcgtac taactgcttc
    22201 agatttttcc agattccttg ccgagaaaga cctttcccat gattattgac aaatacatgc
    22261 atttcagatg cgtcctttac aagtaatgga cgtgcttcgt ctaagtagcg ttccagccat
    22321 tggatcgcat aatcacctaa aggaatgatc cgctctttat ctccttttcc aactgtctgt
    22381 accagtccaa gagaaagatg aagatctccc aatttcaatc caatcaattc actgacgcgc
    22441 atcccagtag catacatgac ttccaagatt gcacgatctc ttatccctaa cgtttttgtc
    22501 gtatcaggtg cttcaatcag cttttctact tctgtcagag tcaatgtact tggtaatttc
    22561 tgtgctttct tcggcgtatc gatatgttgc atcggatcat gatcagtgat acgctcttgc
    22621 cgtaaaaact gatggaatct tctgagactt gagatcatcc gagtaacagt tgctgtcgct
    22681 tttttttctt catgcaactt ctccagaaaa cggatgacta aaaagcgatc gacatcctgc
    22741 catgaagcaa ttttctgctc ctgtagaaaa tgcaagtact gttccaaatc acgttcatag
    22801 ctttgtcgtg tattggcgga caatccccgt tcaatattca agtagtggag ataatcgttg
    22861 atttgctctt tcataataat ccccctcatg cctctctatc atatcagata ccgcttcatt
    22921 ctgatagtct ttttccataa tctgagcaaa gaaaaagccg tctagacact cagacggact
    22981 ggtttctttt ttcggctcta taatttatgg gactgatgaa tcagaattga ttcttcagcc
    23041 ccattttcat tttaggtatt aaaaa
//
LOCUS       DACDKM010000034        22884 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20834_2016
            SAMN12640370-rid9891663.denovo.034, whole genome shotgun sequence.
ACCESSION   DACDKM010000034 DACDKM010000000
VERSION     DACDKM010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640370
            Sequence Read Archive: SRR10040891
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22884)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22884)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742835.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742835.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:04:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,012
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 29 of 75
            Pseudo Genes (incomplete)         :: 33 of 75
            Pseudo Genes (internal stop)      :: 19 of 75
            Pseudo Genes (multiple problems)  :: 5 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22884
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640370-rid9891663.denovo.034"
                     /isolate="E20834_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(239..1531)
                     /locus_tag="IV334_001249"
     CDS             complement(239..1531)
                     /locus_tag="IV334_001249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HAP8943331.1"
                     /translation="MKKKYVQVGLGGRARFFYEAIASTYHQTCELVGFCDVSQTRMDY
                     ANRILVNKYGQKKVKTCMPSQFEQLLDDVRPAEVIITSVDRTHHDYIIRAMKKGYDVI
                     CEKPLTIDEEKAQEIITVQKQTGRSLRVTFNYRYAPYHTKIKELIESDVIGDVFSIHF
                     EWLLNTQHGADYFRRWHRNKHNSGGLLVHKSTHHFDLVNFWLNSRPQKVFAFGGLNFY
                     GMHNAEKRGVEIFYSRCYGSELAKADPFAIDLSANEELREMYLEAEKDSGYIRDRSVF
                     SDDISIEDTLGVTVKYETGAILTYSLNAYMPWEGFNVSFNGSKGRIEMKVIEKSYINA
                     GGDKKDEGAAVLQSIHVYPMFGEPYEVIVEQEAGGHGGGDPRLLDDLFGEEKEEDPWN
                     RAATHVDGILSILTGIAANHSIASGKAVVIDELVSLKE"
     gene            complement(1528..2151)
                     /locus_tag="IV334_001250"
     CDS             complement(1528..2151)
                     /locus_tag="IV334_001250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8943332.1"
                     /translation="MKKPIEEMFLYKLNYYVCRIFLTSLISNALFALVLGCLFTLGLV
                     LSARVVYLIYFLMMYPSYYLSVHYLAEKKVCWRKGKIKRMCFIITLFQLFVIIFAVDW
                     YLLVGKIGAWYLIPFLLCLLLFVSHSFFSTLIDICKEDKADSLVYLIKRNIWLLSKNV
                     KGFLWYLCLLYGLFWCIQPDFLLTALLYPGLPFVTVFCLKEKENDFK"
     gene            complement(2154..2990)
                     /locus_tag="IV334_001251"
     CDS             complement(2154..2990)
                     /locus_tag="IV334_001251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAP8943333.1"
                     /translation="MNNTVTKWFQYGFILLLGLVMIYPVLWMIAGSFKTDQEIMSGSL
                     NLIPETFQWENYAKGWAGFAGISFFTFFRNSFVISAVSTIGTVLSSTCIAYALSRIDF
                     KGKRFWFVVMLVTMMIPAQVILIPQFIIYNRLNLVGTYVPLILPHFFGQAFFIYQIMQ
                     FMVSIPKELDESAIIDGCSKYSVFTKIIFPLLKPSIITTIIIQFYWKWDDFMGPLIYL
                     NKPRSYTVSIAIKLFADAGSSTTYASMFAMSTLSLLPVFLIFLFFNKYLVQGISTSGL
                     KG"
     gene            complement(3007..3894)
                     /locus_tag="IV334_001252"
     CDS             complement(3007..3894)
                     /locus_tag="IV334_001252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP8943334.1"
                     /translation="MKKEKLKRFLDQDNVVGYVFASPFILGFLLITVIPMILSLYYSF
                     TDYRLGNPVSWIGLDNYIKMLSDARFLNSIRVTFLYVFASVPVKLIFGLLIAYLLTRN
                     IKGTTFFRSLYYVPSLIGGSISIALVWRELFSKNGLVNIFLERLGAPALSWFSDQKLV
                     MIPLVLMSAWQFGSAMIIFAAGIKEIPGSYYEAAEIDGANKWQTFYKITLPLLSPIIL
                     YNLVMQTIAAFMTFTQAFIITQGGPNDATNFYALYVYNQAFSFNSMGYASAISWIMLL
                     IMGAVTFLIFKSSKKWVVV"
     gene            complement(3902..5137)
                     /locus_tag="IV334_001253"
                     /pseudo
     CDS             complement(3902..5137)
                     /locus_tag="IV334_001253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738728.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter substrate-binding
                     protein"
     gene            complement(5820..6503)
                     /locus_tag="IV334_001254"
     CDS             complement(5820..6503)
                     /locus_tag="IV334_001254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HAP8943335.1"
                     /translation="MAEQFTPEIMTELRKDIVRVPEIILEASGIKIFGKLIRSIIFTT
                     DIAIIRNTNANAVIAVYPFTPHPAITKSIIEAADIPVFSGVGGGLTQGARSSYMSMFA
                     EAQGSLGVVLNGPTPLETVKEVCRVVDIPVISTVTSKYTEIDEKLAAGISIINISAGK
                     ETAEAVRHFRKKYPELPIIATGGPTEESIAETIQAGANAITYTPPSSGELFSRKMEKY
                     RETEKKNHE"
     gene            complement(6521..7138)
                     /locus_tag="IV334_001255"
     CDS             complement(6521..7138)
                     /locus_tag="IV334_001255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 73 protein"
                     /protein_id="HAP8943336.1"
                     /translation="MAKKKWKKQKRKNIRWPAVVAGVAVILLAFVFSLKNLSSPFTDN
                     QQENEAQSHQQFIERLVPHAQELQDGYGVLPSIILGQAILESNWGKSTLASKYNNLFG
                     IKAYGDQKKVSLETKEFVNEEWITIQGDFKVYDSWEQSMDDHTQLFVQGVDWNPALYE
                     KVITATNYQEAAQALQDAGYATDPGYAQKIIQVIETYQLNQYDRR"
     gene            complement(7148..7972)
                     /locus_tag="IV334_001256"
     CDS             complement(7148..7972)
                     /locus_tag="IV334_001256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HAP8943337.1"
                     /translation="MNKFLGFGAVVIMIAAMGYVIFSDDDSHVQAGNQAVERTSTKTT
                     ASKEKNSQTTSSKVKDKETKDRTSKQLPDVKTDDWKLVLVGPDHKLEKEIDESTQLAS
                     LSNGYLIDKRIAPEYEAFAKAAEAAGFPLVMVSAYRSVSSQQQVFEQNVQDVMSRQGV
                     SEEEATKITKETITEPGYSEHHTGLAVDVVDQNWYNSYPSTVLDASYGDQPGAKWIAD
                     NAAQYGFIVRYPKGHEDITKITYEPWHLRYVGKENAEYITKHQLTLEEYLNELNEK"
     gene            complement(7965..8525)
                     /locus_tag="IV334_001257"
     CDS             complement(7965..8525)
                     /locus_tag="IV334_001257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8943338.1"
                     /translation="MHQVYIDLIMSVLLEQFETEKNFLDEYLLISPEQWQKWKDGEKN
                     LPSEAMQKVKNLFTDYEWMLTQKILRQTIIFPEKRNVAVAEYKQIKTRIAQKWLSSKA
                     AKVELIPLKNNQQAGRYLDLKVSIQYDEWGYDDILNFRLPADVQKQIEGEKIELLDWV
                     NENLEETYVHEANGEREEQRKDFSSE"
     gene            8699..9655
                     /locus_tag="IV334_001258"
     CDS             8699..9655
                     /locus_tag="IV334_001258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HAP8943339.1"
                     /translation="MKKYQWGIVGLGTIAHEFAESFNQETSELTAVASRTSEKAENFA
                     HRYNIPKAYGSYQEMLDDAEIDIVYIAVPNRQHIDHILAALEAGKHVLCEKAITMNKK
                     ELADAMRLAEEKNLILAEAMTIFNMPLYQQLRSIMDTGKLGALKMIQAPFGSYKEPDP
                     KNRFFNPELAGGALLDIGTYAVSFARFFLSSQPEVVASTMVPFETGVDEQSVTILRNK
                     ENELAAVSLTFQAKMPKVGIVAFENGYVTIADYPRADRAEILFTDGTKEFIESGNTSE
                     AMNYEIKNMVHMIEGDLPNRSLFLTKDVIEILDQMQVLWKNM"
     gene            complement(9960..10916)
                     /locus_tag="IV334_001259"
     CDS             complement(9960..10916)
                     /locus_tag="IV334_001259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic acid exporter family protein"
                     /protein_id="HAP8943340.1"
                     /translation="MRIGMRTVKTVISVFFSMVTAGSLSLLYWPAAGIIALLSVGNTK
                     RSTLRTGMNRIAAFILATATALFSFFAVGYTIFGFSLFLFLFIPLASRFKLTEGIVVN
                     SVLVTHYLVEENMSWQLIGNEAGLMAIGLVFALLANVYMPDRTKQLKENQIQIEKEFR
                     NILRQMAEFLLSENKEDVQIKCEHLLTFIRESQEDAREHQENYWLRQPLYYETYFSMR
                     RAQANVIKDMLENLERIQQPAYYGKHIYGLLIYTAETFSESNDGRQILTRIEEVYVLY
                     RQMPLPTSRPEFEDRAELFQFLQSFKSFIEIKAEFSQQKIQK"
     gene            11020..11622
                     /locus_tag="IV334_001260"
     CDS             11020..11622
                     /locus_tag="IV334_001260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HAP8943341.1"
                     /translation="MQFGRFRLGMRTIKSALAVFLCILFFHLTQRGIPMIAALSAVFS
                     LRQDLTTTVSFGKSRIIGNTLGGTLAIIYFFVKSYFHNDFLVELILLPILVIIVIVVS
                     DGINNNSGIISAIATLLLIALSIPQGESALYAIQRVLDTFIGTFIAIGINFFLRPPET
                     EKKEELSEDLVELQKKERLLRTNLQEIEAKIQKQKKNSED"
     gene            complement(11710..13209)
                     /locus_tag="IV334_001261"
     CDS             complement(11710..13209)
                     /locus_tag="IV334_001261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flotillin family protein"
                     /protein_id="HAP8943342.1"
                     /translation="MEIPVVFIAIAIAALILLMLLIVFVAKYQTAKPDEALIISGSYL
                     GSKNVHIDESNNKIKIVRGGGAFVLPVFQRSNRISLLSSKLDVSTPEVYTEQGVPVMC
                     DGTSIIKIGSSVEEIATAAEQFLGKTREELENEAREVLEGHLRSILGSMTVEEIYQNR
                     DKFSQSVQEVASVDLAKMGLIIVSFTIKEVRDKNGYLDSLGKPRIAQVKRDADIAEAE
                     ALKETRIKKAQAEKESQAAELQRQTEIAESLKEKELKLATYKQEQDVAKAKADQAYNL
                     ESARAQQQVIEQEMQIKIVERQKQIELEEKEITRREKQYDSEVKKKADADRYAKEQEA
                     QAQKVKEVTEAEAERFRVEALAEAEANKTRLAGQAEAEAALAKGKAEAEAKQKIANAF
                     KEYGEAAVLSMVIDMLPQLMREAAQPLGNIEKISVVDTGSSSGETSGANRVTNYATNL
                     LSSTQETLKATTGLDLKEIVENLSTRGTTSQVNMYEAPKEAEQNSMKEE"
     gene            complement(13225..13749)
                     /locus_tag="IV334_001262"
     CDS             complement(13225..13749)
                     /locus_tag="IV334_001262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8943343.1"
                     /translation="MIGGIALETIYWYTLLICAGLAVLLIIFGDVFDFDGPLDPMLII
                     PWLTFTSLFGFLGERLTDQSSLFLFIASGGISIVLVFLLNFYILMPMKHAESTMSSSE
                     KTLEGQVAVVITPIPVRGMGEIQLKSVTGSISRPACFYSPQEQTAERGTKVLIIEIKE
                     RICYVMPYESMIKA"
     gene            14047..14796
                     /locus_tag="IV334_001263"
     CDS             14047..14796
                     /locus_tag="IV334_001263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HAP8943344.1"
                     /translation="MNRSSVSDKISSIAKIVANDKLIQKKLQTGDPTHAIQGITNEIM
                     IASNLDFIVVMDPEGIRYSHADPSYIGLPFSSHEDVKKVIGGQEFFSEKEGMLGFGFR
                     YFVPIYYDQEIIGAVCVGLTNNTIDNQVKDGQKMIVFSLIIGLSVGIIGAVFLAQKMK
                     KVLLGLEPQEIAEQLSEKTIIENEVDEGILAISSDKTILLINTPAKNIFYQTEQLLDF
                     EVGKKSVIIYMTFCLKRRLSIKRPFGLNPCI"
     gene            14787..15572
                     /locus_tag="IV334_001264"
     CDS             14787..15572
                     /locus_tag="IV334_001264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HAP8943345.1"
                     /translation="MYLNGIETISSVTPLFSNDRFLGAVATLRNRSEVMKLMEELGGT
                     KQYINSMRAQTHEFMNKLHVISGLIDLKKYEEVTHFIQQLNQNYKKEIGQVSSLIKVP
                     AIAGFLLGKINEAKEQSVEMLLESDSFIPKMEMDDSIHSLLQVLGNLLDNAKEAVLQK
                     HERNGQVRIHLNYDTEAEVFIVTVCDGGVGISEEISEKIFTLGFSTKGINRGFGLNLI
                     HSIVKRHGGFLEFSKQTNGGTIVYLELPKKEDQNDDKCPDNRR"
     gene            15541..16257
                     /locus_tag="IV334_001265"
     CDS             15541..16257
                     /locus_tag="IV334_001265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HAP8943346.1"
                     /translation="MMTNVLIIEDDPMVAELNRTFVESIPNMNVIESFRNVEEGKKYF
                     RQKNADLLLLDVYMPGESGIHFLSWLRQQGNDIPVILITAADDIETIKNVIRYGALDY
                     LIKPFTLQRFQLAIEKYLAFSQTIQTTEKADQKLIDQVFNHTTPIHEIPSFIDLPKGL
                     SKLTLKKISAFILKEDTIFSTKELAQKSSLSRISVKKYLNFLVEQDILNERIKYLEIG
                     QPLTCYELTKNGRKKLTDYC"
     gene            complement(16359..17378)
                     /locus_tag="IV334_001266"
     CDS             complement(16359..17378)
                     /locus_tag="IV334_001266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate dehydrogenase"
                     /protein_id="HAP8943347.1"
                     /translation="MRIACYGVRPNEIDFFEKLNIYHYDLSLYEELLTHDNIVSAKDH
                     DAVLLRGNCIADETNLAKMQEYGIRYVFTRTVGVNHIDLQAAADFGMTVARVPSYSPN
                     AIAELSLTFAMMLLRNTAYTTIRTSFKDFRVDEQMFSREIRNCTVGIIGTGRIGLTEA
                     VLFKGLGAKVIGYDIHQTEAAKKVLTFHSLEELLAKSDIVSIHVPHIPGENDQMINEG
                     FLAQMKKGSILINTARGELQDNEAILRALKSNHLAGFATDVFANETEVFFRSFKPWET
                     IPDPAIQQLVEMYPRVLVTPHVGSNTDEALSNMISTSFENFREIIETGKTKNEVSLPK
                     ARQLR"
     gene            complement(17397..18455)
                     /locus_tag="IV334_001267"
     CDS             complement(17397..18455)
                     /locus_tag="IV334_001267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014372913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HAP8943348.1"
                     /translation="MNLFDIFRSTLTDMSIVSAITSTVFIILLGFVCRKKGIFSESVG
                     KILSKVVLTVALPALAFRAFMQDINTELLMQGINVLIWGLVIYIILILVSKPFFLSYK
                     GDKQTTLRVLTIFGSTTFFGTPIVSAIYGPVGVMYSSIFNIGYRIFLYSYGYIKMSGL
                     KMEAKNIKAMFVNPIIIATFAGLFIWLFQGYLPQVSVTGAEGEISQVAFLRIDQTAPW
                     LFQPMDYLAGLSSPLAWLAIGATLGEVSFKKAASEKTSWYYSLVKVVFVPALNIVILA
                     VLTATNILPVNFEALATIVIMMATPAATVAAAYAISFDREALLASNASLLSTIFAVVL
                     TPVWIVVLQMIHGIGLFS"
     gene            complement(18675..19823)
                     /gene="nagA"
                     /locus_tag="IV334_001268"
     CDS             complement(18675..19823)
                     /gene="nagA"
                     /locus_tag="IV334_001268"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HAP8943349.1"
                     /translation="MRTFIYADKFFLASGVKHAGYLEITDGLFGEYTKELPEGEVNII
                     DQSGKWIAPGLVDTHIHGYMNHDVMDNDAEGIKVMSEGLLSCGVTSFLPTTLTSSKER
                     LKDVAETIGKMYQEAPGAKIKGIYFEGPFFTEEHKGAQNPSYFGDPDLDTFHEWQEAS
                     GGLIKKIALAPERQGVEEFVKQVTEEGVVVSLGHSNATLEEAQKAVEAGASVFVHAFN
                     GMRGLNHREPGMVGALLSLEHVFSELICDGHHVHPNAAGILMEKAGHDHVALITDCMM
                     AGGMPDGNYNLGEFPVVVKEGTARLESGNLAGSILKLKEAIKNVVDWGLATPEQAVMM
                     ASLIPAISCKIDDTCGKIAQGRDADFIVLNPDMTLEATYLDGVERFHA"
     gene            complement(19899..20735)
                     /locus_tag="IV334_001269"
     CDS             complement(19899..20735)
                     /locus_tag="IV334_001269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP8943350.1"
                     /translation="METLIESFGTIIKEIRKEQKMTQKMLSHDICSQSVLSRIENNEE
                     LPNVLVMMQICQRLGVTIDHVMNLSRKTTRTNDKLFELLDSCFQKRDYRKLEQVLKSS
                     DISENLYQATDFQRYYYYLGVCEFTLRQNISQSLYYLKEALSYSSQKERIHVSDTEIQ
                     LISCIGKIYGVAGKTAEARYYLSRSIQLLHQSADERSKSEFSQIFYNYGNFLFHQNEI
                     DEALEQVNQGIYWAQEKNSYYYLNDLFVLKSLIYKRKEEPERAAFYEGLAQAVKQISK
                     NL"
     gene            complement(20919..22136)
                     /locus_tag="IV334_001270"
     CDS             complement(20919..22136)
                     /locus_tag="IV334_001270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HAP8943351.1"
                     /translation="MREFEKSNKLEGVSYDVRGPVLEEAERMQEEGISILKLNTGNPA
                     PFGFEAPNEVIRDLIMNARDSEGYSDSKGIFSARKAIEQYCQLKHFPNVTINDIYTGN
                     GVSELITMCMQGLLDNGDEVLVPMPDYPLWTASIALAGGTPVHYICDEEAEWYPDIDD
                     IKSKITSRTKAIVIINPNNPTGALYPKELLEEIVEVARQNNLIIYSDEIYDRLVMDGL
                     EHIPIATLAPDLFVVTLNGLSKSHRVAGFRVGWMVLSGDKSHVKGYIEGLNMLSSMRL
                     CSNVLSQQIIQTALGGYQSVDELLLPGGRIYEQREYIYNAINDIPGLSAVKPKAAFYI
                     FPKIDTKRFNILDDEKFVLDFLHEHHILLVHGGGFNWNQPDHFRIVYLPKMDDLKQTA
                     KKMREFLATYRQK"
     gene            22519..22602
                     /locus_tag="IV334_001271"
     tRNA            22519..22602
                     /locus_tag="IV334_001271"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:22553..22555,aa:Leu,seq:gag)
BASE COUNT         6831 a         4813 c         3968 g         7272 t
ORIGIN      
        1 gtggtttact tatttttttg taaaatctaa tgatttttaa tgcattattc ctttttgttc
       61 gggtttaaat agttattttt attttctcct atctaaaaaa gcagcccaca taaaaagaag
      121 gcatgacaaa aagcatgtca tagccccttt ttccgaataa atagtgttca aaagacagcc
      181 tatcggcaat aaggcaagat tttataaact acgggaagta atttccgtaa atctattctt
      241 attcttttaa gctgacaagc tcgtcaatca ctactgcttt tcctgaagca atagaatgat
      301 ttgctgcgat tcctgtcaaa atcgacaaaa taccatctac gtgagttgct gcccgattcc
      361 acggatcttc ttctttttct tcaccaaata gatcatctaa taaacgcgga tctcctccgc
      421 catgtccgcc cgcttcttgt tcgacgatta cctcatatgg ctcaccgaac atcggataga
      481 catggatgga ctgaagaacg gctgctcctt catccttttt atctccacct gcattgatgt
      541 aagacttttc gatcactttc atttcgatcc gtcctttgct gccattgaaa gaaacattga
      601 atccttccca cggcatatac gcgtttagcg aatacgtcaa aatcgcacca gtttcatatt
      661 tgaccgttac gcctagtgta tcttcaatag agatatcatc ggaaaataca ctgcggtcac
      721 gaatatatcc actgtctttt tctgcttcta aatacatttc tcgcaattcc tcattggctg
      781 acaggtctat agcaaatgga tcagctttag ctagttcgct tccatagcat cgactgtaaa
      841 atatttctac tccgcgtttc tccgcattat gcatcccata aaagtttaat ccaccgaatg
      901 caaatacctt ttggggacgg ctgttcaacc aaaaattcac tagatcaaaa tgatgggtag
      961 atttatgaac aagaagcccg ccgctattgt gcttatttcg atgccatctt ctgaaataat
     1021 ctgccccgtg ttgcgtattt aacagccatt cgaagtggat agaaaacaca tcaccaatca
     1081 catcactttc tatcagttcc tttatcttcg tgtgatacgg tgcataacga taattaaaag
     1141 tgactcgcag gcttcttccc gtctgttttt ggaccgttat tatttcttga gctttttcct
     1201 catcgatagt cagaggtttt tcgcaaataa catcatatcc ttttttcatt gcacgaatga
     1261 tataatcatg atgcgttcga tctactgacg taataatgac ttcagccggc cgtacgtcat
     1321 ccagtagttg ctcaaattgt gatggcatac atgtctttac ttttttctgt ccatacttgt
     1381 tcacaagtat ccgatttgca taatccatcc ttgtttgaga tacatcacaa aaaccgacta
     1441 gttcacaagt ttgatgatac gtagaggcaa ttgcctcata aaaaaaacgc gctctcccac
     1501 caagtccgac ttgtacatat tttttcttca tttaaagtca ttctcctttt cttttaagca
     1561 aaatactgtg acaaatggca gccctggata cagtaatgca gtgagtaaaa aatcaggctg
     1621 tatacaccag aacagtccat acaaaaggca taaataccac agaaacccct tgacgttttt
     1681 tgacaacagc cagatattcc ttttgatcaa ataaacaaga ctatctgctt tatcttcttt
     1741 gcagatatca attagcgtac taaaaaaact atgggataca aaaagcaaca gacacaatag
     1801 aaaaggaatc agataccaag ctccaatctt tcccactaat aaataccaat caactgcaaa
     1861 aataatgaca aacagctgaa acaacgtaat gatgaaacac atccgtttga ttttcccttt
     1921 cctccaacat accttttttt ctgccaagta atggacagat agataataag aaggatacat
     1981 catcaaaaaa taaatcagat agacaacacg agcagacagc actaatccta aagtgaacaa
     2041 acatcctaga actaaagcga aaagagcatt actgataaga ctggttaaaa aaatccgaca
     2101 gacatagtag ttgagcttgt aaagaaacat ctcttctatt ggctttttca tattcagccc
     2161 ttcagaccgc ttgtactgat cccctggaca agatatttat tgaagaataa gaaaatcaga
     2221 aaaaccggta gcagcgacaa cgtagacatc gcaaacatac tcgcgtaagt cgtactagat
     2281 ccagcgtctg caaataattt gatggcaatc gataccgtgt aagatctcgg cttgtttaaa
     2341 taaatcaatg gtcccatgaa gtcgtcccat ttccaataaa actggatgat gatcgtcgtg
     2401 atgatcgaag gtttcagcaa aggaaaaata attttggtaa agacactata cttgctgcat
     2461 ccgtcaataa tcgctgattc atctaactct ttggggatgc tgaccatgaa ttgcatgatc
     2521 tggtaaatga aaaatgcttg tccaaaaaaa tgtggtaaaa tcagcggcac atacgtccca
     2581 accaaattca gccgattgta aataataaac tgcggaatca aaatcacttg tgcaggaatc
     2641 atcatcgtta ctaacatgac gacgaaccag aatctttttc ctttgaaatc gattctcgat
     2701 aatgcgtaag caatacaagt cgagctcaga actgtcccta tcgtcgaaac tgcagagatg
     2761 acaaacgaat tcctaaagaa agtaaaaaat gaaattccag caaaccccgc ccatcctttt
     2821 gcgtaatttt cccattgaaa agtctctgga atcaaattca gcgaaccact cataatctct
     2881 tgatccgttt taaaagaacc tgcgatcatc cataagaccg gataaatcat gactagacct
     2941 aatagcaaaa taaaaccgta ttgaaaccat ttcgttactg tattattcat ttcttcctcc
     3001 tctcattcac acaacgaccc atttttttga tgatttgaaa atcagaaagg tgacagctcc
     3061 cataatcaac agcatgatcc acgaaatagc actagcatac cccatagaat taaaagaaaa
     3121 agcttggtta tacacataaa gagcataaaa gttcgtggca tcattcggac caccttgggt
     3181 aatgataaat gcctgcgtaa aagtcataaa cgccgcgatt gtctgcataa ccaagttata
     3241 caaaatgatc ggcgaaagaa gaggtaacgt gatcttgtaa aacgtctgcc atttgttcgc
     3301 tccatcgatc tctgccgctt cataatacga gcctgggatc tctttgatac ctgcagcaaa
     3361 gatgatcatt gccgaaccga attgccaagc agacatcaaa acaagaggga tcatgaccag
     3421 tttttgatcg ctaaaccagg aaagcgcagg tgcgcctaat cgttccagga aaatattcac
     3481 taacccgttt ttagaaaaca actcccgcca aactagagcg atagaaatac tgccaccgat
     3541 caaactaggc acataataca aagaacgaaa gaaagtcgtt cctttgatat tcctcgttaa
     3601 aagataagca atcaatagac caaagatcag tttcacaggt acagatgcaa atacatagag
     3661 aaaagtgact cggatggagt tcaaaaaacg ggcatcactc agcatcttta tataattatc
     3721 caagccgatc catgagactg gatttcccaa acgataatcg gtgaacgaat aataaagtga
     3781 caagatcatc ggtatcactg ttatcaatag aaatcctagg atgaatggag aagcaaatac
     3841 gtaaccgacc acattgtctt gatccaagaa ccgttttagt ttttcttttt tcatgccttc
     3901 cttctgctgt tacttcttca taaatgactt tttcaactaa aagattgtat tggtcttgaa
     3961 tttccggatt caaagcacta tcaaagacat tcacttcgcc gttgtccata tcggaaatca
     4021 tttctaaata atcaaatgtc gcttgcaaag ttgcatcatc tgcacgagcg agctgttctc
     4081 gaatattcga catgattggt acaccacgtt ctccttttaa aatttcattg gcttcttcac
     4141 tgttttgaaa gaaatcaata aacttagcag cttcttcttt atgggctgag ctttttgcca
     4201 tactcagcat ttgcgaagaa cgtaaaggga taccagattc tccatctgct tttctgcgag
     4261 gtggattaat caactgaatc tccatatctt caggagcccc tgacaatatt tctgtcaact
     4321 ggttacttga tagaaacacc attgccgctt ctctatccct aaccgcctga aaagacagat
     4381 ccgtcgtact tgcagctcca ccacctggtt ctggatgggc ccctttgtct accagatttt
     4441 tacgcagctg gaaaaaatct gttagatagt ctggattttc aaatcccaag ctgttagata
     4501 gatcttcccg attataaaaa ttcaatcctt tctcaacttg tggtatttga ataatacagc
     4561 cagctataaa atcttcgaat cttgaaatag catagattcc taattcttca gtgatttgat
     4621 agctgatttg ttcaaattca tcccatgtcc aatttgaagc tggttcaggg atatttaatt
     4681 gttcaaatat atttttattg taagcaatcg caaacgaatt caccccgtta gacagtccaa
     4741 ctaattgccc gtcgtaagtc gtcgttgcta aataggattc atctgcatta gaaatatcaa
     4801 ttatcccttg ctccacatat tcatctaatg gttcaatctg attgatgtat tgaggaaatt
     4861 gattgttcag ttggaaaatg tcccagatat catctgatgc tgctagtgta cctaattttg
     4921 tgtaatatcc ttcataatca taaaattcat aatcgatcgt cacatgggga tttttttctt
     4981 cataaagctc gattactttt actgttgcat cgtgtcggac ttgcgaaccc caccaagcca
     5041 tgttcagcgt gatttttttg tcatcttctt gcccacaagc cgtcaagaag aataacataa
     5101 cacttaaagc taagatgatt ctcattcttt tcttcattct ctgcacctct acttccattg
     5161 ttatttttca atatactgta tttttataca gaacatattt attaaacgat ctttttcaag
     5221 gggaataaaa accaagactt gccttatcgt tctgcctagg atgaacaact caatactttc
     5281 aagaaaaatt agatagtgct gattcttctt tcatcgctaa aaacacttct tgcttcaatg
     5341 atgggaggat aattacgcaa agaaaaaagg atatcagaca actgcccaat atccttgaca
     5401 tagctaaata agcatgcttc ttcgtgtgcg ctcttcccta aaaaacagaa aaataactag
     5461 taattctgta tgaaattagg tacaatatac acgtcgatgc acaagcattg actatggcaa
     5521 cttgattggt cagttcccgc tgacttttca agccccgaca atttagctgc tactaaattg
     5581 tcgggtgcct tttttagttt ttattcgttt ttcattctat agaaagcgct tcaataaatc
     5641 aagacatttt tttgttagaa tgctaataat aacaaaaggt tttaactctt cttatgtaca
     5701 ttacaggtaa aaaaacaaaa aaatttctct tttttttgaa ttctgaacaa atagtactgg
     5761 agtacaaaag acgaggaaga gccactatct gctcttcctc gtcttttgat tctcacctgt
     5821 tattcatgat tctttttctc agtttcccga tatttttcca ttttacgact aaaaagctct
     5881 ccgctacttg gtggcgtata tgtgatggca tttgctcctg cttgaatggt ctcagcaata
     5941 ctttcttccg ttggaccacc tgtcgcaatg ataggaagtt caggatattt tttacgaaaa
     6001 tggcgtactg cttctgcagt ttctttacct gcactgatat tgataatcga aataccagct
     6061 gctaactttt catctatttc tgtatatttc gacgttaccg tactgataac tggaatatca
     6121 actactcgac aaacttcttt tactgtctct aaaggcgtag gaccgttcaa aacgacccct
     6181 aacgaacctt gcgcttctgc gaacatgctc atgtaggaag aacgagcacc ttgtgtcaaa
     6241 ccaccaccta caccggagaa aactggaatg tcagctgcct caataatgct tttagtaatc
     6301 gctggatgag gtgtaaaagg atacacagca attactgcat tcgcatttgt attacgtatg
     6361 atcgcaatat ccgtagtaaa gatgatcgaa cggatcaatt ttccaaatat cttgatcccg
     6421 cttgcttcta aaatgatttc cggtactctt acgatatctt ttctcaattc cgtcatgatt
     6481 tctggcgtaa attgttctgc catcagccac ttctcctttg ctatctccgg tcatactgat
     6541 tcaattgata cgtctcgatg acctggatga ttttttgcgc ataaccgggg tctgtcgcat
     6601 aacctgcatc ttgcaatgct tgtgcggctt cctgatagtt ggtggccgta atcacttttt
     6661 catacagcgc aggattccaa tctactcctt ggacgaacag ttgtgtatgg tcatccatgg
     6721 actgttccca tgaatcatac actttgaagt ctccttggat agttatccat tcttcgttaa
     6781 caaattcctt tgtttccagg cttactttct tctgatcccc ataagccttg atcccgaaaa
     6841 gattattgta tttgctagcc aaggtacttt ttccccaatt tgattctaat atagcttgtc
     6901 ctaaaatgat actaggaagc actccataac catcttgtag ttcttgggca tgcggaacaa
     6961 gccgttcaat aaattgctga tgggattgcg cttcattttc ttgctgatta tcagtaaaag
     7021 gagaagacag attcttcaag gaaaaaacaa aggctaagag tatcactgct acaccggcaa
     7081 ccactgctgg ccaacggata ttcttgcgct tttgcttttt ccactttttt ttcgccatga
     7141 tcaattccta cttttcattt aattcattca gatattcttc tagcgtcaat tggtgtttgg
     7201 tgatatactc agcgttttct ttaccgacat aacgtaagtg ccaaggttca tatgtgatct
     7261 tagttatatc ttcgtgtcct tttggataac gaacgataaa tccatactgc gcagcattat
     7321 ctgcgatcca tttagctcca ggctgatctc cgtaacttgc atccaataca gtagaaggat
     7381 aactgttgta ccagttttga tcgacgacat cgactgccag acctgtgtgg tgttcgctgt
     7441 atcctggctc cgtaatcgtt tctttagtaa tctttgtcgc ctcctcttct gatacacctt
     7501 gacgactcat cacatcttgg acattttgtt cgaaaacttg ttgttgggat gatacagacc
     7561 ggtaagctga gaccatcact aatggaaatc cagctgcttc agcagccttg gcaaatgctt
     7621 catattctgg agcaatgcgt ttgtcgatca agtatccgtt acttaatgat gccaattgag
     7681 tagactcatc aatttctttt tctaatttgt gatctggacc aaccaaaact agcttccaat
     7741 cgtctgtttt aacatctggc aattgtttgg acgtacgatc ttttgtttct ttgtccttta
     7801 ccttgctgct ggttgtttga ctgttctttt ctttactagc agttgttttg gtgcttgttc
     7861 gttcgactgc ttgattgccg gcttgaacat gcgaatcatc atcgctaaaa ataacatagc
     7921 ccattgctgc gatcataatc acaactgctc caaatcctaa aaatttattc acttgaaaaa
     7981 tcctttcgtt gttcttcacg ttccccattc gcctcatgaa cgtaagtttc ttccagattt
     8041 tcattcaccc aatcgagaag ttcgattttt tctccttcga tctgtttttg aacatctgct
     8101 ggcagacgaa aattcaaaat atcatcatag ccccattcat catattgaat acttactttc
     8161 aagtcaaggt atctaccagc ctgctgatta tttttcaaag ggatcagctc gacttttgct
     8221 gctttgctgc taagccattt ttgcgcgatg cgtgttttga tttgcttgta ctctgcaacg
     8281 gcaacgtttc gtttttcggg aaaaataatc gtttgacgca aaatcttttg cgttagcatc
     8341 cattcataat cagtgaaaag attttttact ttctgcatcg cttcagatgg cagatttttc
     8401 tcgccatctt tccatttttg ccattgttct ggactgatca gcaagtattc atctaaaaaa
     8461 tttttttcag tttcaaactg ttctaataat acactcataa taagatcaat atatacttga
     8521 tgcacgttta tcccctcctt ttaactattc tacttaagtt ttgatcaaaa agcacctctt
     8581 aaatcgctgc tttcattttt tttaatgttt caagtcctct cctctttatt cttcgtgatt
     8641 tttgttataa taatagacag acaagaaact caatccaact atgaaggagc gttttcagat
     8701 gaaaaaatat caatggggaa tcgttggtct tggtaccatc gcacatgagt ttgccgaaag
     8761 cttcaatcaa gagacgagcg aactaacagc agttgcttcc agaacttcag aaaaagcgga
     8821 aaacttcgct caccgttaca atattccaaa agcttacgga agctatcaag aaatgttaga
     8881 cgatgctgaa atcgacatcg tgtacattgc tgtaccaaat agacagcata tcgaccatat
     8941 cttggcagca ttggaagcag gcaaacacgt tctatgtgaa aaagccatca cgatgaataa
     9001 aaaagaactg gctgacgcta tgcgcttagc agaagaaaag aatttgatct tagcagaagc
     9061 gatgacaatc ttcaacatgc cactttatca gcagttacgc tcaataatgg atacaggaaa
     9121 actgggggct ttaaaaatga tccaagcacc tttcggcagt tacaaagaac cagaccctaa
     9181 aaaccggttc ttcaatccag aattagctgg aggagctctg ttggatatcg gtacgtatgc
     9241 tgtatctttc gctcgtttct ttttgagctc tcaacctgaa gtagttgcct ctacaatggt
     9301 tccttttgaa acaggcgtgg acgaacaatc cgtcactatc ttgcgtaaca aagaaaatga
     9361 acttgctgct gtcagcttga cttttcaggc aaaaatgcca aaagtcggta tcgttgcctt
     9421 tgagaacggt tatgtgacga ttgctgatta cccgcgtgct gatcgtgcgg agatcttatt
     9481 tacagatggt acaaaggaat tcatcgaaag cgggaataca tctgaagcaa tgaattacga
     9541 aataaagaat atggtacaca tgattgaagg tgacttgccc aatcgctctc tcttcttgac
     9601 aaaagatgtc atcgaaatct tggatcaaat gcaagttcta tggaaaaata tgtaatttta
     9661 aaaggttgtg aaaaagctgt tctgcatcaa gtaataagaa cagccaaaca aaaatagctc
     9721 ctcctatttt tcgtttggct ggacttatta ccgcagagga aagcttttga acaccgttta
     9781 agaaagaggt tgtgagccta ccgttcagct ccgagtcaca aggagcaatt tcaaaaaaca
     9841 gcttctcatg tttgttgaat attgtgactt gtgaccgagg agttgacagg agaacaccgt
     9901 ttaaagaaga ggttgtgaaa tcagcttttt gacctgaata ttagctgaat tcacaacctc
     9961 tatttttgga ttttttgttg ggagaattct gccttgattt caataaaact tttaaaagac
    10021 tgcaaaaact ggaacaattc tgctcgatcc tcgaattcag ggcgactagt cggcagaggc
    10081 atttgccgat acaatacata aacttcttca atacgtgtta aaatttgtct gccatcattt
    10141 gactctgaaa atgtttctgc agtataaatc aataaaccat agatatgttt tccataataa
    10201 gctggttgct ggattctctc tagattttcc agcatgtctt taatcacatt ggcctgtgct
    10261 cttctcatag agaagtaagt ttcgtagtat agcggctgtc tgagccagta gttttcttga
    10321 tgttctctgg catcttcttg gctttcgcga atgaatgtca gtaaatgttc gcatttgatc
    10381 tgcacatctt ctttattttc tgataaaaga aattctgcca tttgtctcaa aatatttcga
    10441 aattcttttt cgatctgtat ttggttttcc ttcagctgct tcgtacgatc tggcatgtaa
    10501 acatttgcaa gcaaagcaaa tactaatcca atcgccatta atcccgcttc attgccgatc
    10561 agctgccaag acatgttttc ctccactaaa taatgagtga ccaaaaccga atttactact
    10621 atcccttcgg ttaatttgaa gcgagaggca agcgggatga acaggaataa aaaaagactg
    10681 aagccaaaaa tagtataccc aacagcaaaa aaactgaaca gtgctgttgc cgtagctaat
    10741 ataaaggctg cgattcgatt catgcccgta cgcaatgtag agcgcttggt atttcctaca
    10801 cttaataatg caatgatccc tgcagctggc caatacaaca gcgataagct acccgctgtg
    10861 accatcgaaa agaagacact gatcactgtt ttgactgttc gcattcctat acgcatttac
    10921 ttcccccttt tttattttaa ttatacttga ataatgaaaa actagctatc agacaaaagt
    10981 ttaggtatga tagtaaaaag accaaaaagg agaatctcta tgcaatttgg ccgttttcga
    11041 ctaggtatgc gtacgatcaa aagtgcctta gctgtttttt tatgtatctt gttttttcat
    11101 ttgactcaac gaggaatccc gatgattgct gcattatctg ctgttttttc tttgcgtcag
    11161 gacttgacta caaccgtttc ttttggaaag tctaggatca tcggcaatac attaggtggt
    11221 acattagcta tcatttattt ttttgtcaaa agctattttc acaatgattt tttggtagaa
    11281 ttgatcttgc ttcctattct agttattatt gtgatcgtcg tgtcagatgg tattaacaat
    11341 aattccggta tcatttccgc aatagctacg ttgttgctga ttgcgttgag catccctcaa
    11401 ggcgaatccg ctttgtatgc tatccaacgt gtactagata cttttatagg gacattcatt
    11461 gcaatcggta tcaatttctt tcttcgtcct cctgaaacag aaaagaagga agaactatca
    11521 gaagatttgg tcgaactcca aaagaaagaa cgattgctaa gaacaaattt acaagagata
    11581 gaagctaaga tccaaaaaca aaagaaaaat agcgaggact aattttcttt tgcaataaaa
    11641 taaaaaaatg gagagaagaa ctttaaaaag tcttcctcca tttttgattg aacggtgaag
    11701 tgtactactt tattcttcct tcatcgagtt ttgttctgcc tcttttggcg cttcatacat
    11761 atttacttgg ctagtagtcc cacgtgtcga caaattctct acgatttcct tcaagtcaag
    11821 tcctgttgtc gcttttagtg tttcttgagt agaagaaagc aaatttgtgg cataatttgt
    11881 cactcggttc gctccactgg tttctcctga actgcttcct gtatcgacaa cagagatttt
    11941 ttcgatattt cctagcggct gtgctgcttc gcgcatcaat tgcggtagca tatcgataac
    12001 catactcaag acagctgctt cgccatattc cttgaaggca ttggcgatct tttgttttgc
    12061 ttcggcttct gcttttcctt ttgctagtgc tgcttcggct tctgcttgtc cagctaaacg
    12121 tgtcttattg gcttccgctt ctgccagtgc ttccacacgg aatcgttctg cttcggcttc
    12181 agtcacttct ttgacttttt gagcttgtgc ttcttgttct ttagcgtacc gatcggcatc
    12241 tgcttttttc ttcacttccg aatcatattg cttttcacga cgtgtgattt ctttttcttc
    12301 cagttcgatt tgtttttggc gttctacgat tttaatctgc atttcctgtt cgattacttg
    12361 ttgttgtgct cgggcacttt ctaaattgta ggcttggtct gcttttgctt tagcaacatc
    12421 ttgttcttgt ttgtaggttg ctaatttcag ttctttttct tttaatgatt ctgcgatctc
    12481 tgtttgtcgc tgtaattcag ctgcttgtga ttctttttct gcttgagctt ttttgatacg
    12541 cgtttctttt agagcttccg cttctgctat atctgcatct cttttgactt gggcgattct
    12601 tggtttccct aaagagtcaa gataaccgtt tttgtctctg acctctttga tagtgaaaga
    12661 aacgatgatc aaccccattt ttgcaaggtc tacagaagcg acttcttgta cgctttgact
    12721 gaacttatcg cgattttgat agatttcttc tacagtcata gaaccaagaa tcgagcgcaa
    12781 gtgtccttct aatacttctc ttgcttcatt ttccagttct tctcttgtct tgcctaaaaa
    12841 ctgttctgct gctgtagcaa tctcttctac agatgaaccg attttgatga tcgaagtacc
    12901 atcacacatc accggtacgc cttgttcggt atacacttca ggggtggaga cgtccaattt
    12961 actcgataat agactgatcc gattcgaacg ttgaaaaact ggcaagacga atgcgccacc
    13021 tccacgcaca attttgattt tgttattgct ttcatcaatg tgcacatttt tgcttcctaa
    13081 ataacttcca ctgatgatca acgcttcatc cggttttgct gtctggtact tcgctacaaa
    13141 gacgatcagc aacatcagta aaatcaaagc tgcgattgcg atcgcgataa aaacaactgg
    13201 tatttccatt taacacacgc tcctttaagc ttttatcatg ctttcataag gcatgacata
    13261 acagattctt tccttgatct caatgatcaa tactttcgtt ccccgctcag ctgtctgttc
    13321 ttgggggctg taaaaacaag ctggacgact aatggaaccg gtaacacttt tcaactgaat
    13381 ttctcccata cctcgtacag ggatcggtgt gatcacaaca gctacttgtc cttccagagt
    13441 tttttcagaa gacgacatcg ttgattcggc atgtttcatc ggcatcaata tatagaaatt
    13501 aagcaaaaat accaacacta tcgatatgcc tccactagca ataaataaaa ataaagatga
    13561 ttgatctgtt aaacgctcac ctagaaaacc aaataagctt gtgaaagtta accaagggat
    13621 aataagcatc ggatcaagcg ggccatcaaa atcgaataca tctccaaaaa taatcaatag
    13681 gactgccaat cctgcacaaa tcagcaatgt ataccaataa atcgtctcaa gtgcgattcc
    13741 ccctatcata tacacgcctc cttccatttc tacgtctatc ataccaatgt ttgaaaattc
    13801 tgtatagctt cttttaccga ctcagtggtg ttttttatag gaaggttgtg tttatcgttg
    13861 acaatttgtt aaatttttgt tttcatgtta aaattttcac aataaattct ttcgaggtag
    13921 cctatgtttc ctttttttaa gaaaaaacca ttaaagctcc agaacacgat catccttgtc
    13981 gtttgtttct ccacattcgt ttctttgatt gtttctgcca tattgatcag gaattttatt
    14041 ctttcgatga accgatctag tgtgtcagat aaaatctcta gcattgctaa aatcgttgcc
    14101 aatgataaac tcatccaaaa aaagctgcag actggtgacc cgacacatgc gattcaagga
    14161 ataacaaatg aaatcatgat tgcatcgaat ttagatttta tcgttgtcat ggaccctgaa
    14221 ggcattcgat attctcacgc agacccaagt tacatcggtc ttcctttctc aagtcatgaa
    14281 gatgtgaaaa aagtgattgg aggacaagaa tttttctctg aaaaagaagg aatgttaggg
    14341 ttcggcttcc gctatttcgt ccctatttat tatgatcaag agattattgg tgcagtttgt
    14401 gttggcttaa caaacaacac gattgacaat caagtcaaag atggacaaaa aatgattgtt
    14461 ttttcattga ttattggact tagcgtaggc atcataggcg ccgtttttct tgcccaaaaa
    14521 atgaaaaaag tactgcttgg acttgaacca caagaaatag cagaacagtt aagtgaaaaa
    14581 acgattatcg aaaatgaagt agatgaagga atcttagcca tctcttctga taaaactatc
    14641 ctcttgatca atacacctgc aaaaaacatt ttctaccaaa cagagcagct attggatttc
    14701 gaagtaggaa aaaaatcagt gataatctat atgacgtttt gtttaaagag acgattgagc
    14761 atcaaacgac cgttcggact caatccatgt atttaaatgg gatagaaact atttctagcg
    14821 ttacacctct ttttagcaat gaccgttttt taggagcagt cgcaacttta cgaaatcgat
    14881 cagaagtaat gaaattaatg gaggagcttg gggggacgaa acaatatata aactccatga
    14941 gagctcaaac ccatgaattc atgaataaac ttcatgtcat ttctggtttg attgatttga
    15001 agaagtatga agaagtcacc cattttatcc agcagctaaa tcaaaattat aaaaaagaga
    15061 ttggccaagt ctcaagtctg atcaaagtac ctgcaattgc cggtttttta ttaggcaaaa
    15121 taaacgaagc caaagaacaa tctgttgaaa tgctgttaga aagcgattct tttatcccta
    15181 aaatggagat ggatgactcc atccattcac ttttgcaagt gttagggaac ttacttgata
    15241 atgcaaaaga agccgtgcta caaaaacatg aaagaaacgg acaggtccgg attcatttaa
    15301 actatgacac agaagctgaa gtattcatcg tgaccgtctg cgatggcggt gtcgggatat
    15361 cagaagaaat ctcagaaaaa atatttacgc taggtttttc gacgaaggga atcaatcgtg
    15421 ggtttggatt gaatctcatc cattcaatcg tcaaacgcca cggtggattt ttagaatttt
    15481 ccaaacaaac aaatggcggt acaatcgtct atctagaatt accaaaaaag gaagatcaaa
    15541 atgatgacaa atgtcctgat aatcgaagat gatccaatgg ttgcagaatt gaatcgtact
    15601 tttgtggaaa gcatccctaa tatgaacgta atcgaaagtt tcagaaatgt agaagaagga
    15661 aaaaaatatt tcagacaaaa aaacgcagat ctgctgctgc ttgatgtata catgcctggc
    15721 gaaagtggta tccatttttt atcttggctt agacagcaag gaaatgatat cccagtgatt
    15781 ttgatcactg ctgcagatga tatcgaaacc ataaagaatg tgatccgcta cggtgcctta
    15841 gattatttga tcaaaccgtt cactttgcaa cgttttcagc tggcgatcga aaagtatctt
    15901 gctttctctc agacaatcca aacgactgaa aaagccgatc agaaattgat cgatcaagta
    15961 ttcaaccaca ccactcccat tcacgaaata ccatcattca tcgatttgcc taaaggtctg
    16021 tcaaaactga ctttaaaaaa aataagtgca tttattctaa aagaagatac tattttttca
    16081 acaaaagaac tggctcaaaa aagctcactg tcacgtatat ccgtcaagaa atatttgaat
    16141 tttttagtag aacaagatat attgaacgaa agaatcaaat atttggaaat cggccagccg
    16201 ctaacctgtt atgaattgac aaagaatggg cggaaaaagc tcacagatta ctgttgagtc
    16261 atcgaattat cccatacaaa atagtccaag tatacagaag taacaactgt ttacttggac
    16321 tatttttaaa cattttattg acgaatatcc atgttttctt atcttaattg acgggcttta
    16381 ggaagagata cctcattttt cgtttttcca gtttctatta tttcacgaaa gttctcgaaa
    16441 cttgtcgaaa tcatatttga taatgcctca tctgtatttg aacctacatg cggggttaca
    16501 agaactctag ggtacatttc tactaattgc tggatagcag ggtctggaat cgtctcccat
    16561 ggcttgaagg atcggaaaaa aacctctgtt tcatttgcaa aaacatcggt tgcaaaacca
    16621 gctaaatgat tgcttttcaa agctcttaaa atagcttcat tatcttgaag ctctccacgg
    16681 gctgtattaa tcaagatgct accttttttc atttgagcta aaaatccttc attgatcatt
    16741 tgatcgtttt cacctgggat atgaggtaca tgaatactga caatatcgct tttggctaat
    16801 aactcttcaa gcgagtggaa cgtcaatact tttttagcag cttctgtctg atgaatatcg
    16861 tagccgatca ctttggctcc tagtccctta aacaaaacag cttccgtcaa ccctatcctg
    16921 cctgttccta tgatccctac tgtgcaattt ctgatttcac gactaaacat ttgctcatcc
    16981 actcgaaaat ctttgaaaga tgttcggatc gtcgtatacg cagtatttct caaaagcatc
    17041 atggcaaaag tcaaagataa ctctgctatc gcatttggcg aatacgatgg tacacgggct
    17101 actgtcatac caaaatcagc tgccgcttgt agatcgatat gattgactcc cacagtccgt
    17161 gtaaaaacat aacgaatccc atattcctgc atttttgcaa gattggtctc atctgctata
    17221 caatttcccc tcaacaatac agcatcatgg tcctttgctg acacgatatt gtcatgtgtc
    17281 aacagttctt cataaagact gagatcataa tggtaaatat tcaatttttc aaaaaaatct
    17341 atctcgttcg gacgaacacc gtaacatgcg attctcatac tttcttcctc catttcttat
    17401 gaaaataaac ctatgccatg aatcatttgt aatacaacga tccatacagg tgtcagtacc
    17461 acagcaaaaa tagttgacag taaggatgca ttggaggcta aaagtgcctc cctatcaaaa
    17521 ctgatagcat aggctgctgc cactgttgca gctggtgtag ccatcatgat aacgattgtt
    17581 gccagtgctt caaaattgac tggtagaata ttcgttgccg tcaaaactgc tagaatcacg
    17641 atattcaatg caggtacaaa taccactttg actagactgt aataccatga agttttttca
    17701 gatgcagctt ttttgaagct tacttcacct aatgttgcgc cgattgctag ccacgccaat
    17761 ggtgaactta aaccagctaa ataatccatc ggctgaaata gccacggtgc agtctggtca
    17821 attcgcaaaa acgctacttg gcttatctct ccttctgcac ctgtaactga cacttgtggc
    17881 agataccctt ggaacagcca aatgaaaagg ccggcaaaag tagcgataat gattggattg
    17941 acgaacatcg ctttgatatt tttagcttcc atcttcaagc cactcatttt tatgtatcca
    18001 taagaataca aaaagattcg gtagccaata ttgaaaattg aggaatacat cacgccgaca
    18061 ggaccataaa tcgcactgac gataggcgtt ccgaaaaagg tcgtagaacc aaagatcgtc
    18121 aggacacgca acgttgtttg cttatcccct ttatagctta agaaaaaagg ttttgaaact
    18181 agaataagaa taatataaat aactaatccc caaatcaaga cattgattcc ttgcatcagt
    18241 aattctgtat tgatatcttg catgaaagct cgaaatgcca gtgctggcaa agccactgtt
    18301 aagacgactt tcgatagaat ttttccaaca gattcagaaa aaatcccctt ttttctacaa
    18361 acaaacccta ataatataat aaaaactgtt gaagtgattg cactgacaat cgacatatct
    18421 gtcaaagtcg atcgaaaaat gtcaaataag ttcatctttc tcctcctaat tatgttcatt
    18481 tattcacaat aaaaaaagct tttattttgc taatacgtat taacattttt aattttatct
    18541 ttacattgtt acttattgaa caataggaaa ttaaagaaac ttttgaaact tatgtaaagt
    18601 gatttttact agaacacaaa aaaaccttaa aaccaaactt aagaagttta gttttaaggt
    18661 taacttaaat tatattatgc gtggaaacgt tccacgccat ctagataagt tgcttccaat
    18721 gtcatatcag ggttcaatac gataaagtct gcatcgcgtc cttgagcgat ttttccacat
    18781 gtatcatcga ttttacagct gattgctgga atcagactag ccatcatgac tgcttgttct
    18841 ggtgttgcta atccccagtc tacaacattt ttaattgctt ctttcaattt caatatactt
    18901 ccagctaagt tgccagattc tagacgtgct gttccttctt ttactactac tggaaattcg
    18961 cctaaattgt aatttccatc aggcatccca ccggccatca tacagtctgt aattaatgcg
    19021 acatgatcat gtcctgcttt ttccattagg attcccgcag catttggatg tacatgatgg
    19081 ccatcacaaa tcaattcaga aaagacatgt tctaaagata ataacgcacc caccatacct
    19141 ggttcgcgat gattcaatcc acgcatacca ttgaaagcat ggacaaatac acttgcgcct
    19201 gcttcaacag ctttttgcgc ttcttctagt gtagcattac tgtgtcctag agaaacaact
    19261 actccttctt cagtcacttg tttgacaaac tcttctacac cttgacgttc cggtgctaaa
    19321 gcaatttttt tgattaaacc acctgaagct tcttgccatt catggaaagt atccaaatca
    19381 ggatccccga aataacttgg gttttgtgcc cctttgtgtt cttctgtgaa gaaaggacct
    19441 tcaaaataga ttcctttgat tttagctcct ggtgcttcct gatacatttt gccgattgtc
    19501 tcagctacat cttttaaacg ttctttgctt gacgtcaatg ttgttggtaa aaatgaagtc
    19561 actccacaag ataacagacc ttcagacatc actttgatgc cttctgcatc attatccatt
    19621 acatcatgat tcatatagcc gtgaatatgt gtatctacca aaccaggagc aatccatttg
    19681 ccgctttggt caatgatgtt gacttctccc tcaggtagtt ctttggtgta ttcaccaaaa
    19741 agtccatcgg taatttcaag atagcctgca tgtttgacac ctgacgctaa aaagaatttg
    19801 tcagcataga taaaagttct cattctattc tctccctttg tttttctacc tctaaagtac
    19861 tcttatttag agaagaagtc aagaatggtc tacaccattt acaaattttt tgaaatttgt
    19921 ttcacagctt gtgctaagcc ttcatagaat gctgctcttt ctggttcttc tttacgcttg
    19981 tatattaagc ttttaagcac aaataaatca ttgaggtaat agtagctatt cttctcttga
    20041 gcccaataga ttccttgatt cacctgttct aacgcttcgt cgatttcatt ttgatggaat
    20101 aagaaatttc cataattata aaaaatttgc gaaaattcac tttttgatcg ttcatcagct
    20161 gattgatgca gcagttggat gcttcgactc aaatagtagc gtgcttcagc cgtttttcct
    20221 gccacaccat atatttttcc gatacaacta atcagttgta tttctgtgtc agaaacatgg
    20281 attctttctt tttggctaga gtagcttaat gcttctttca aataataaag ggactgagag
    20341 atattttgac gcaacgtgaa ttcgcaaaca ccgagataat aataatagcg ttgaaagtca
    20401 gttgcttgat acagattttc agaaatatca gaagatttta gtacttgttc taacttccga
    20461 taatcacgtt tttggaaaca actatccaac agttcaaata atttatcgtt tgtccgagta
    20521 gtttttcgag agagattcat cacatgatca atcgttaccc ctagccgttg acaaatctgc
    20581 atcatcacca atacattcgg cagttcctca ttattttcga tccgacttaa tacactttgt
    20641 gaacaaatat catgcgaaag cattttttgc gtcatttttt gttcttttcg aatctcttta
    20701 atgatcgttc caaaggactc gattaacgtt tccataagta tcctccccaa aatatgcgta
    20761 ttcgcattct attcgtattt tactttagga agtcgcaaac ttcaagaaaa gacttgcaac
    20821 taaataatat actttagtta tttaagaaaa ctccactaga aagagagcct cagtgtgttt
    20881 gtctgtggcc ctcctactga aaaatttggt ttttaaaatc atttttggcg ataggttgct
    20941 aggaattctc tcattttctt agctgtttgt ttcaaatcat ccattttagg aagataaacg
    21001 attcgaaaat gatctggctg gttccaatta aatccaccac catgtacaag tagaatatga
    21061 tgttcatgta aaaagtccaa aacgaatttt tcatcatcca aaatattgaa tcgctttgta
    21121 tcaatttttg ggaatatata aaaagcagct tttggtttta cggctgaaag accaggaata
    21181 tcattgattg cattatagat atattctctt tgttcataga tccttccgcc tggcaggagc
    21241 agctcatcga cactttgata accgcctagc gcggtttgga taatttgctg agacaataca
    21301 ttggaacaca aacgcatgga ggacaacata ttcagccctt ctatatagcc tttgacgtgt
    21361 gacttatctc cacttaggac catccagcct actcgaaatc ctgctacacg atgagatttg
    21421 gatagaccgt tcagcgtaac aacgaacaaa tcaggcgcta atgtagcaat cggaatatgt
    21481 tctagaccat ccatcactaa gcgatcatag atttcgtcgg aataaataat caggttattt
    21541 tgtcgagcta cttcaacgat ttcttctaat aattcttttg gataaagtgc cccagtagga
    21601 ttattaggat taatgataac aatagctttt gtccgactcg ttatttttga tttgatatca
    21661 tcaatatcag gataccattc tgcctcttca tcgcaaatat aatgaacagg tgtgcctcca
    21721 gctaatgcaa tagaagccgt ccacaacgga tagtccggca ttggaaccag tacttcatct
    21781 ccattgtcca gcaacccctg catacacatc gtgatcaatt cgctcactcc gttgccggtg
    21841 tagatatcat tgatcgtcac attcggaaaa tgtttcagtt ggcaatattg ctcaattgct
    21901 tttctagcag aaaagatccc cttggaatct gagtagcctt ctgagtcacg ggcattcatg
    21961 atcaaatcac gaatgacttc gtttggtgct tcaaatccaa aaggtgcggg atttcccgtg
    22021 ttcaatttta aaatactaat gccctcttct tgcatccgct ccgcttcttc tagaacggga
    22081 ccgcgaacat cgtaactgac accttctaat ttgttggatt tttcaaattc tctcatcact
    22141 tagccaatcc ctcatttcaa aaagttagaa ctttccaaat tttttgttat ttactatttc
    22201 aatatcatac tccatcctag aaaattttgc cacttattta ggaaaaacgt aagtattaaa
    22261 aaaacacaaa caagcgttga gaacttaatt gtcatgatag tcattccata gattcgctgc
    22321 ccaatccatt gcaattccac aaatgattcc tagcataaaa ccaagcaaaa aacgtccgaa
    22381 tgcaagtcca atcaatacac ctaagatcat tcccagtcct gaaaattctc cgtcatattt
    22441 ctccatctta ataagtcctc ccatttttga gcttgacaac tatgtaaaac ctcgctatca
    22501 ttagtgtgtt attattacgc agatgtgttg gaattggcag acaagcatga ttgagggtca
    22561 tgtgagcgtt agctcgtgtg ggttcgagtc ccatcatctg cactacaact cgtgaagtta
    22621 attttgcttc acgagttttt ttgtttgaac gtttctttat gccattatat acatatcact
    22681 gtatccttta caaaaagaac gtacaatcat gtaaacagcc tgaggtgagt atatgaaggt
    22741 cggaaacaag ctactcgtag aaacttgcaa gtgttaaaac aattaatcga aaaacgagta
    22801 aaatccctca cctaatacta aagcgaggga ttttttgtat attatctgtt tatcaccgtt
    22861 attataaagg tacaaatacc aagc
//
LOCUS       DACDKM010000035        22801 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20834_2016
            SAMN12640370-rid9891663.denovo.035, whole genome shotgun sequence.
ACCESSION   DACDKM010000035 DACDKM010000000
VERSION     DACDKM010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640370
            Sequence Read Archive: SRR10040891
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22801)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22801)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742835.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742835.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:04:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,012
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 29 of 75
            Pseudo Genes (incomplete)         :: 33 of 75
            Pseudo Genes (internal stop)      :: 19 of 75
            Pseudo Genes (multiple problems)  :: 5 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22801
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640370-rid9891663.denovo.035"
                     /isolate="E20834_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            9..233
                     /gene="nrdH"
                     /locus_tag="IV334_001272"
     CDS             9..233
                     /gene="nrdH"
                     /locus_tag="IV334_001272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-like protein NrdH"
                     /protein_id="HAP8943352.1"
                     /translation="MNVKIFSKNNCMQCKMVKRFLTENQIAFEEVNIDEQPEAVDWLK
                     AQGFQSVPVITSDAETVIGFRPDQLRKLAS"
     gene            392..2551
                     /gene="nrdE"
                     /locus_tag="IV334_001273"
     CDS             392..2551
                     /gene="nrdE"
                     /locus_tag="IV334_001273"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HAP8943353.1"
                     /translation="MSLKNLQDVSYFKLNNEINRPVNGQIPLNKDKEALVAFFEENVK
                     PNTMTFLSIMEKINYLIDQEYIEAEFIQLYSPSFIEKLYQFLDEQHFSFKSFMAAYKF
                     YSQYALKTNDGSAYLETYEDRVAFNALYFANGDEELALALADEMIHQRYQPATPSFLN
                     AGRKRRGELVSCFLTQVTDDMNSIGRSINSALQLSRIGGGVGITLSNLREAGAPIKGY
                     EGAASGVVPVMKLFEDSFSYSNQLGQRQGAGVVYLNVFHPDIIAFLSAKKENADEKIR
                     VKTLSLGVIVPDKFYELTRKNEDMYLFSPYSVEKEYGVPFSYVNITAEYDHLVANPNI
                     RKQKIKARDLENEISKLQQESGYPYVINIDTANRENPIDGKIIMSNLCSEILQVQTPS
                     VINGKQEYEVLGTDISCNLGSTNIVNLMQSPNFGQSVRTMTRALTFITDASDIDVVPP
                     IQNGNKLNHTIGLGAMGLHTYLAKEQMEYGSEDSLDFTNIYFMLLNYWTLVESNNIAR
                     ERKQVFHNFEKSAYADGTYFEKYVTGEFQPKSEKVKALFEGIFIPTAEDWNALKQAVM
                     KDGLYHQNRLAVAPNGSISYINDTSASIHPITRMIEERQEKKIGKIYYPAPYLSNETI
                     PYYTSAYDMDMRKVIDVYAMAQQHVDQGMSLTLFMRSEIPEGIYEWKTTTKQTTRDLN
                     ILRHYAFHKGIKSIYYVRTFTDDAEEIGSNQCESCVI"
     gene            2601..3566
                     /gene="nrdF"
                     /locus_tag="IV334_001274"
     CDS             2601..3566
                     /gene="nrdF"
                     /locus_tag="IV334_001274"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HAP8943354.1"
                     /translation="MSETYYEAINWNEIEDIIDKSTWEKLTEQFWLDTRIPLSNDLDD
                     WRTLSQLEKDTVGHVFGGLTLLDTVQSESGMDQLRKDVRTPHEEAVLNNIQFMESVHA
                     KSYSSIFSTLNTKKEIEEIFDWTNTNPYLQKKAERINEIYKHGTPLEKKIASVFLETF
                     LFYSGFYTPLYYLGNNKLANVAEIIKLIIRDESVHGTYIGYKFQLGFNELPEAEQETL
                     KDWMYNLLYELYENEERYTEELYDPIGWTEEVKTFLRYNANKALMNLGMDPLFPDTAN
                     DVNPIVMNGISTGTSNHDFFSQVGNGYLLGHVEAMKDDDYLIGLE"
     gene            complement(3655..4794)
                     /locus_tag="IV334_001275"
     CDS             complement(3655..4794)
                     /locus_tag="IV334_001275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HAP8943355.1"
                     /translation="MQSRFKQSKLFFWTVELVLTIIGVYFILQMPNIFSPILKMLSAV
                     FLPLLVAGFIYYMFDPIVVFLEKHRIPRVFGFLLTFLVVIILVVLTVMNVVPQLMEQT
                     IQLTQSLPMYADETSKWLNELAQREEFKNFNLEEQLASANLSLSNLFNIILVSLSASV
                     SKIISFMMQFFILLFTVPFILLFMFKDGHKFIDALSHFFPKAIRKELRQTVKELNETL
                     SAYISSTILDALIIGVMSFLAMRIFDQPYSLLLAVICGITNIIPYVGPFIGAVPAIIV
                     GLFISPFQALYMALSILVIQQLDGNLIKPLLFGKSMNIHPLTIILVLIGAGSVAGILG
                     MLICIPVYAVIKTIILNIRKIYLLRRMEHVSEKLDSPKVLEETSE"
     gene            complement(5103..5816)
                     /locus_tag="IV334_001276"
     CDS             complement(5103..5816)
                     /locus_tag="IV334_001276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HAP8943356.1"
                     /translation="MNDMVLTIPEIILRLGIAMLFGGAIGFERQYKNRPAGMRTHILV
                     CMGACIIALIQVEIASGALRDALEYPQLAGTIRSDEARLIAQVVSGVGFLGAGTIIVT
                     KQSVTGLTTAASLWAIAGLGIAIGMGYYSIAIISFIGIAIALTVVKRVIHVPTTKKLE
                     IQFIHRKETKDFLTDYFEEKNIVIEDVNFDVKFVDDYRVYKNIYTIDLPKGLTYADVI
                     EELSVHKNITKLHLVSIAQ"
     gene            complement(6070..6771)
                     /gene="nagB"
                     /locus_tag="IV334_001277"
     CDS             complement(6070..6771)
                     /gene="nagB"
                     /locus_tag="IV334_001277"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HAP8943357.1"
                     /translation="MEIIRVKNAEEGGKKAFEIIKEGMNKGAKVLGLATGSTPVTLYK
                     EMTSSDLDFSEMTSVNLDEYVGLGGSDEQSYRHFMDVQLFDKKPFKETFVPDGKAEDL
                     EAACKKYDQIIDAHPIDIQILGIGQNGHIGFNEPGASFDGTTSVVDLTESTINANKRF
                     FDKAEDVPTKAVSMGIGSIMKSKEIVLMAFGESKADAIKGMIEGPVTNHLPASVLQNH
                     DNVVVIIDEEAASKL"
     gene            7005..8537
                     /locus_tag="IV334_001278"
     CDS             7005..8537
                     /locus_tag="IV334_001278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HAP8943358.1"
                     /translation="MSILTLNNITQQFGDKILYEGVDLQLNAGEHLGLIGQNGAGKST
                     LIKIITGEILPDDGQIHWQKNIHKGYLDQYVEVDETLTIEEFLKTAYAKEYEKEEKIA
                     FLYQTYSETFDDSLLEKAGKLQTELDQGVFYQIDTLVAEMSSGLGIDVLGLDSLLKNL
                     SGGQRSKVILAKLLLENPDVLILDEPTNHLDDQHIQWLTQFLQDFDGAYIVISHDQEF
                     LDQITTHIADIEFGKITKYTGHLKQALKQKEQNRESYLRQYHAQQKQIEKTEAYIRKY
                     KAGSRSTMAKSRQKQLDKIERLTPPASASKAVFDFPFSPIVTTLAVETTDLVIGYDRP
                     LLAPINLTIRFGENIAIRGFNGIGKSTLLKTLIGEIEKISGDFHFPDNTKINYFSQDL
                     YWENPLETPLQYLSSQFPKATIKELRRQLAKAGLVNQLASEPLTTLSGGEQTKVKLAQ
                     LTMNQGNLLILDEPTNHIDQETKESLQDGIQKFPGTVIIVSHEQEFYQDLVQRVIEIE
                     PK"
     gene            complement(8728..9930)
                     /locus_tag="IV334_001279"
     CDS             complement(8728..9930)
                     /locus_tag="IV334_001279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP8943359.1"
                     /translation="MKSNEKKPLTTSLILLLSITCGVVVANMYYIQPIGTKVAASLSV
                     STSAVGILTMLTQLGYALGLLFLVPLGDVVDRPKLIIRMAALSAVSLLAAFFAPSFTL
                     FACASFLIGLLSIVPQIIIPYGAVLAGPKARGKVMGTLLSGLLVGILLSRTVSGIVAS
                     LFSWRMIYLFALCAVGILTLLLYWKMPRSVQQSSTAVSYLDSLKSLPHLVASQRLLRE
                     AAVSGFFMFGTFSIFWSTLIFYISSPAYNWGTFEAGILAIFGLSGAVAAPIVGRLADS
                     YSERKIVYMGLVMQTISFIALLVGGSHLAILLLAIILLDVGNQFGQVANQTRVQGLGE
                     AASNRNNTVFMFMYFIGGAAGSLLGTTMWQQYGWLGVTVSGLVFQGCAFFFQTVVFKG
                     KRNLENKK"
     gene            complement(10012..10701)
                     /locus_tag="IV334_001280"
     CDS             complement(10012..10701)
                     /locus_tag="IV334_001280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HAP8943360.1"
                     /translation="MKKKIIRLILISLGIILILVVGYRLYLEYQTDIQLFLNPRASKA
                     ELLAHIRSHGTFASILLIVLTAVMCAVPGIPTSVVGVIAGLSFGPLTGAIINILGNAL
                     GNITAIFLMHRLKFLDKKTETNHWVQAIRQMKHPKIGVMLGYMIPVIPSSMVNFAADA
                     MKLPLQQIILSIVIGVIPSSVLYACGGEALFHGYNKTAVTLVASVTVLVGLVVFIYKD
                     RKKKQGKQKHK"
     gene            complement(10807..11175)
                     /gene="rplL"
                     /locus_tag="IV334_001281"
     CDS             complement(10807..11175)
                     /gene="rplL"
                     /locus_tag="IV334_001281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HAP8943361.1"
                     /translation="MALNIENIVAELKEATILELNDLVKAIEEEFGVSAAAPVAVAAA
                     GGAAAAEEQTEFTVELTAAGDQKVKVIKAVREATGLGLKEAKAVVDGAPAPVKEGVSK
                     EEAEELKAKLEEVGASVTVK"
     gene            complement(11228..11731)
                     /locus_tag="IV334_001282"
     CDS             complement(11228..11731)
                     /locus_tag="IV334_001282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HAP8943362.1"
                     /translation="MSEAIIAKKAAIVDEVSEKFTAAASVVVVDYRGLTVDEVTRLRK
                     QLRDANVEMKVIKNSILSRAAKKAGLEGMDEVFTGPTAVAFSNEDVVAPAKIMDEFAK
                     EAKALEIKGGIIEGKVSSLEEITALAKLPNREGLLSMLLSVLQAPVRNVAYAVKAVAD
                     KEDGDAA"
     gene            complement(12141..12830)
                     /gene="rplA"
                     /locus_tag="IV334_001283"
     CDS             complement(12141..12830)
                     /gene="rplA"
                     /locus_tag="IV334_001283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HAP8943363.1"
                     /translation="MAKKSKKMQDALKKVDSSKVYPVAEAVALAKETNIAKFDATVEV
                     AYRLNVDPKKADQQIRGAVVLPNGTGKTQTVLVFAKGDKAKEAEAAGADFVGDDDMVQ
                     KIQNGWFDFDVVVATPDMMATVGRLGRVLGPKGLMPNPKTGTVTMDVTKAVNEVKAGK
                     VTYRVDKAGNIHVPIGKVSFDDEKLVENFAAIHDVLLKAKPSAAKGQYMKNVTITTTF
                     GPGIHVDQASF"
     gene            complement(12934..13356)
                     /gene="rplK"
                     /locus_tag="IV334_001284"
     CDS             complement(12934..13356)
                     /gene="rplK"
                     /locus_tag="IV334_001284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HAP8943364.1"
                     /translation="MAKKVEKIVKLQIPAGKATPAPPVGPALGQAGINIMGFTKEFNA
                     RTADQAGLIIPVVISVYEDRSFTFVTKTPPAAVLLKKAAKIEKGSGEPNKNKVAKVSS
                     DQVREIAELKMEDLNAANVEAAMRMVEGTARSMGITVE"
     gene            13699..14367
                     /gene="sdaAB"
                     /locus_tag="IV334_001285"
     CDS             13699..14367
                     /gene="sdaAB"
                     /locus_tag="IV334_001285"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent,
                     subunit beta"
                     /protein_id="HAP8943365.1"
                     /translation="MKDLRYRSVFDIIGPVMIGPSSSHTAGAARIGKIIRSIFGEQPD
                     SVDIYLYESFAKTYRGHGTDIALVGGLLGMEPDDERLADSLKLAYEAGMEVLFVPKSE
                     KAEHPNSVKMLLKKGTRKLSVTGISIGGGNIQISELNGFKISLSMGTPTLIVVHQDVP
                     GMIAKVTSILSETNTNIGTMTVTRESKGENAIMIIEVDDPQVEETVKRLKQLPNIDSV
                     NYFD"
     gene            14380..15252
                     /gene="sdaAA"
                     /locus_tag="IV334_001286"
     CDS             14380..15252
                     /gene="sdaAA"
                     /locus_tag="IV334_001286"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent,
                     subunit alpha"
                     /protein_id="HAP8943366.1"
                     /translation="MFLSIEELVDQAKAYPSVAELMIAVEMEMSGRSRSHIIEIMEKN
                     LSVMEQSINEGKEGVTSVTGITGGDAKKLDDYLNEADFLSGETILKAVRNAVAVNEVN
                     AKMGLICATPTAGSAGVVPGVLMAVRDRLDLTHEQQLEFLFTAGAFGLVIANNASISG
                     AEGGCQAEVGSASAMAAAALVCAKNGTPHQAAQAVAITLKNMMGLICDPVAGLVEVPC
                     VKRNALGSSQAFISADMALAGIESVIPPDEVVAAMYQVGRQMPSIFKETAEGGLAVTP
                     TAKRLAEEILNKRA"
     gene            complement(15343..16362)
                     /locus_tag="IV334_001287"
     CDS             complement(15343..16362)
                     /locus_tag="IV334_001287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate oxidase"
                     /protein_id="HAP8943367.1"
                     /translation="MEAGAFGYIRGGSEDEWTMKENTTSFNTKKIMPRILRGIDSADL
                     HTSVFGIELDTPIIQAPSAAQGLAHEKGEADTAKGVAAAGSIFSISTYANTTIKDAAD
                     AAPGAPQFFQLYMSKDDRFNEFILNKAVEAGAKAIILTADSTLGGYREEDVINQFQFP
                     LPMPNLAAYSEQSASGNGEGKGIAEIYAAAKQGLTPDDIKTIKEITHLPVIVKGIQSP
                     EDAVIAISAGADGIWVSNHGGRQLDGGPASFEVLPKIAEVVNKRVPVIFDSGVRRGEH
                     VFKALASGADLVAIGRPVIYGLNLGGAEGVTSVFEHLNKELSITMQLAGTKTIDEVKN
                     TKLMD"
     gene            complement(16535..17422)
                     /locus_tag="IV334_001288"
     CDS             complement(16535..17422)
                     /locus_tag="IV334_001288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HAP8943368.1"
                     /translation="MTEPIKNLCKRATAAALVLISLFALSGCQTTKINTTEVSDKEKT
                     AESSKVIEKTTIPTLFFHGYSGTENSFKGMLRRLEKNQQAVKELVLTVGTDGQIQATG
                     DLSGKADNPMVQVLFEDNKNNEWNQAEWIKTCLLYLKENYGIDKVNIVGHSMGGVSAL
                     RYLGTYGQDTSLPQVQTLTAIGAPFNDFVDDSAQSLTDELAKGPAVVSNRYQDYQQMI
                     GNIPITTRFFLIAGQLDETDLSDGTVPLNSALAVYALLKQRGNEIEEKVVTGENASHS
                     MLHENQEVDQLVSRFIWKE"
     gene            complement(17499..18044)
                     /gene="nusG"
                     /locus_tag="IV334_001289"
     CDS             complement(17499..18044)
                     /gene="nusG"
                     /locus_tag="IV334_001289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HAP8943369.1"
                     /translation="MESFEKNWYVLHTYSGYENKVKANIESRAQSMKMADFIFRVVVP
                     EEKETEVKNGKEKEIVHKTFPGYVLVEMIMTDASWYVVRNTPGVTGFVGSHGAGSKPA
                     PLLPEEVNHILRSIGMSLRQNDLEVEVGEVVKIIEGAFAGLEGQVTEVDGEKEKLKVN
                     IDMFGRETSTELDFDQVDKID"
     gene            complement(18145..18315)
                     /gene="secE"
                     /locus_tag="IV334_001290"
     CDS             complement(18145..18315)
                     /gene="secE"
                     /locus_tag="IV334_001290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HAP8943370.1"
                     /translation="MKFLKSVKDEIKQVTWPSRKQLRKDTLVVIETSIIFGLMFYVMD
                     TAIQSLFGWILK"
     gene            complement(18339..18491)
                     /gene="rpmG"
                     /locus_tag="IV334_001291"
     CDS             complement(18339..18491)
                     /gene="rpmG"
                     /locus_tag="IV334_001291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HAP8943371.1"
                     /translation="MAKKKAALACSVCGSRNYTKSVSEGKSGERLEINKFCKYCNQYT
                     LHKETK"
     gene            18653..19297
                     /locus_tag="IV334_001292"
     CDS             18653..19297
                     /locus_tag="IV334_001292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HAP8943372.1"
                     /translation="MLLKTLVYKKKDLTTVTETSTLEEALKILETSGYRCVPILDSSG
                     NIFRGNIYKMHIYRHKANGGDMTLPVTHLLKNATKFIYLDTSFFKVFFTIKELPYIAV
                     LDSENHFYGILTHSTLLNMLSQSWSVEQGSYVLTIASTGKQGDLAAIAKIISKHSSIA
                     SCITLDIEKDEYIRRTLITLPANTEAKVCETIVNHLEKKNYKVVEIENLQNQEA"
     gene            complement(19353..19856)
                     /locus_tag="IV334_001293"
     CDS             complement(19353..19856)
                     /locus_tag="IV334_001293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HAP8943373.1"
                     /translation="MQKTKSRQRLSVIVVNGIIMALYLALTILVAPVSSGPIQFRISE
                     SLNHLVVFNRKLLWGVLGGVIVYNAFFGFGIMDVVFGGGQTLLSLGLTALLQNKVKNV
                     KMRLVLNTIFFTISMGLIAYMLVPTGGQAFWTTYGTLALSEFIVMAVSAPLMYYLDRA
                     LDFEKRV"
     regulatory      complement(19869..19915)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00522"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="PreQ1 riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF00522"
     gene            20453..21418
                     /locus_tag="IV334_001294"
     CDS             20453..21418
                     /locus_tag="IV334_001294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family lipid kinase"
                     /protein_id="HAP8943374.1"
                     /translation="MKFHYHILINPSAGSGNGYKVAERILPVLKNKHIDYTLHYSEYK
                     GHDAEIAETLAKETLIPWIEEEQKTIDTFPLLIIVGGDGTLHQVLDTFYQMEVEFPVA
                     YIPAGSGDDFARGAGLPKNPKKGLQLILAAQSPEKVHIMAYEEKISEKKGIAVNNFGI
                     GLDAAIVHATNHSSTKKRLNKYNLGSFSYLFSILRALFTQKGFPILVDAGGKQLSFSN
                     AFLCTATKHPFFGGGIAIVPTADASKPVIDFVVVERINFFKILWLILLLIQKKQLKSK
                     YFHHYTTNRLRIVSTTPQHGQEDGEEMEKRPFDITITTTKQLFWC"
     gene            complement(21517..22689)
                     /locus_tag="IV334_001295"
     CDS             complement(21517..22689)
                     /locus_tag="IV334_001295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP8943375.1"
                     /translation="MTLYKTIWTLLKANKSNLLIGVIITVVATFFFAQNIDVENTSLQ
                     DAKIALISEEESPVVDGLRKYLNDKETVVTLDGTSQKEIDDALYFNRVNIILHLPADF
                     TEQVEVGKMPTIKIQSRPDAFSKTVVTQQVNTFLQTLILFQEEDKTIDAAMEQTQMAL
                     SVTGEVELSAGYSQRMKKLLTGTTFNFLSYGLFLSIFSGFSVINLAFNRKEISKRNQS
                     APITKRNLNRKVTFSLISYSLLLFSGFLLLMVLLVIHTSWDITVWYHILNTFLFLLPI
                     ISFSACITSLVKNSETSGGIKDIFITGSCFIGGVFVPAEYLPEMVSKIAAFTPTYWFV
                     QNNNLISETLSYNQTFAESFWFNGCVLVAFAAVFSMIQFILGKERNYRWQPNWAKN"
     gene            complement(22676..>22801)
                     /locus_tag="IV334_001296"
     CDS             complement(22676..>22801)
                     /locus_tag="IV334_001296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP8943376.1"
                     /translation="KLYFYTDLASFYTNLLWLIGFIFLFALGNAWMERGVRYDAL"
BASE COUNT         7125 a         4584 c         4097 g         6995 t
ORIGIN      
        1 cactcaccat gaacgtaaaa atcttttcta aaaataattg tatgcaatgt aagatggtca
       61 aacggttttt aacagaaaat caaattgctt ttgaagaagt caatatcgat gaacagcctg
      121 aagcagtaga ctggttgaaa gcacaaggat tccaaagtgt tcctgtcatt acttctgatg
      181 cagaaacagt tatcggattc cgtcctgatc agttgcgtaa acttgccagc taacatgact
      241 aaaagaatca aaacgtgata catggatttg aagggcttga gacagcacca gtttttgtcg
      301 caaacttcca aatccttttt taaagcttaa aaacaatgca atgatttgca atcaagcatc
      361 atcaaatacg aaatgaaggt tgggcgtacg tatgagtctt aaaaatttac aagatgtcag
      421 ttatttcaag ctgaataatg aaatcaatcg gccagtcaat ggtcaaatcc ctttgaataa
      481 ggataaagaa gctttagttg ccttttttga ggaaaatgtc aaaccaaata cgatgacttt
      541 tctatctatt atggaaaaaa tcaattactt gatcgatcaa gaatatatag aagcagaatt
      601 cattcaattg tattcaccat catttattga aaagctatat caatttttgg acgagcaaca
      661 tttttctttc aaatctttta tggctgctta caaattttat tctcaatatg cgttgaaaac
      721 caacgacggt tccgcttatc tggaaactta tgaagatcga gtagccttca atgcgcttta
      781 ctttgcgaac ggggatgaag aattagcact tgctcttgca gacgaaatga tccatcaacg
      841 atatcagcca gctacacctt ctttcttgaa tgctggacgt aaacgtcggg gcgaattggt
      901 gtcttgtttc ttgactcaag tcactgacga tatgaactct atcgggcgct caatcaactc
      961 tgctttgcaa ttgtcccgta ttggtggcgg cgtagggatt actttatcaa atcttcgtga
     1021 agccggcgct cctatcaaag gatatgaggg tgctgcaagt ggtgtcgttc cagtcatgaa
     1081 attattcgaa gatagtttca gctattctaa tcaattaggt cagcgtcaag gagctggcgt
     1141 ggtttatttg aacgttttcc atccagacat tattgctttc ctttcagcaa aaaaagaaaa
     1201 tgcagatgaa aagattcgcg taaaaactct ttctcttggc gtgatcgtac ctgataaatt
     1261 ttatgaattg acacgtaaaa acgaagatat gtatttattt agtccatata gtgtcgaaaa
     1321 agaatatggt gtcccattct cttatgtaaa tattacagct gagtatgatc atctagttgc
     1381 aaatccaaac attcgcaaac aaaaaatcaa agcgcgtgat cttgaaaatg aaatctcaaa
     1441 attacaacaa gaatctggct atccttacgt aatcaatatt gatacagcga accgcgagaa
     1501 tccaatcgat ggcaagatca tcatgagtaa tttatgttca gagattttac aagttcaaac
     1561 gccttctgtg attaacggca aacaggaata tgaagtatta ggaacggata tctcatgtaa
     1621 cttaggctcg acaaatatcg tgaatttgat gcaaagccct aactttggtc aatctgtgcg
     1681 tacgatgact cgtgcattga catttattac agatgcatct gatatcgatg tcgtaccacc
     1741 gatccaaaac ggaaataagc tgaatcatac gattggatta ggtgccatgg gcttacacac
     1801 ttatcttgca aaagaacaaa tggaatatgg ctcagaggat tcattagact ttacaaatat
     1861 ttacttcatg ctgttgaatt attggacact tgttgaaagc aacaatatcg ctcgtgaaag
     1921 aaaacaagtt ttccataatt ttgaaaaatc agcttatgct gacggcactt atttcgaaaa
     1981 atacgtaact ggagaattcc agccaaaatc agaaaaagtc aaagcattgt ttgaaggcat
     2041 ttttatccca actgctgaag attggaatgc tttgaaacaa gcagtaatga aagatggact
     2101 gtatcatcaa aatcgcttgg cagtagcacc aaatggctca atttcatata tcaacgatac
     2161 gagtgcaagt attcatccta tcactcgcat gatcgaagaa cgccaagaga agaaaatcgg
     2221 taaaatttat tacccagcgc cttatctatc caatgagacg attccgtatt acacttctgc
     2281 ttatgatatg gacatgcgta aagtaatcga tgtttatgca atggcacaac aacacgtcga
     2341 tcaaggcatg agtttgactt tattcatgcg ttctgaaatc ccagaaggca tttatgaatg
     2401 gaagacaaca acgaagcaaa cgacacgtga tttgaatatt ttaagacatt acgctttcca
     2461 caaaggaatc aaatccattt attacgtacg tacctttact gatgatgcag aagaaatcgg
     2521 aagcaatcaa tgtgaaagct gcgtgatcta atatcataga tagattcgct tattcatatt
     2581 ttaattaaag gagcattctc atgtcagaaa catattatga agcaataaac tggaatgaaa
     2641 ttgaagatat cattgataaa tccacttggg agaagctgac agagcaattt tggctagata
     2701 cacgtatccc actttcaaat gacctagatg attggcgtac attgtctcaa ttggaaaaag
     2761 atacagtagg acatgtattt ggcggtttga cactgctaga tactgtccaa tctgaaagtg
     2821 gcatggatca gttgcgtaaa gatgtccgca ctccccatga agaagccgtt ttgaacaata
     2881 ttcaattcat ggaatccgtt catgccaaaa gttattcttc tatttttagt acactgaata
     2941 cgaaaaaaga aatcgaagaa atttttgact ggacaaatac gaatccttat ctgcaaaaaa
     3001 aagctgagcg tatcaatgaa atctacaaac acggtacacc gctagaaaag aaaattgcca
     3061 gcgtcttttt agaaacattc ttgttctatt caggttttta tactcctctt tattatttag
     3121 gaaacaacaa attggctaac gtagcagaga tcatcaaact aatcatccga gatgaatctg
     3181 tacatggaac ctacatcggt tacaaattcc aattaggatt caacgagtta cctgaagcag
     3241 aacaagaaac gttaaaagac tggatgtaca atcttcttta tgagctttac gaaaacgaag
     3301 aacgttatac agaagaatta tacgatccaa tcggctggac agaagaagtc aagacattcc
     3361 tacgctataa tgccaacaaa gcattgatga acctagggat ggatccactc tttccagata
     3421 ctgccaatga tgtcaaccca atcgtaatga acgggatttc aacaggcaca agcaaccatg
     3481 acttcttctc acaagtcgga aacggctatt tgttaggtca tgtagaagcg atgaaagacg
     3541 acgattattt gattggattg gaataaggat ttattttaat tcaaaaattt ttcgaaaaat
     3601 aaaaagagtt tagcgtacag atattttaaa tgtacactga actctttttt atgcttattc
     3661 acttgtttct tccagcactt ttggactgtc taacttttcc gaaacatgtt ccattcttct
     3721 caataaatag attttccgga tatttaatat tatcgtctta atcactgcat agacaggtat
     3781 gcaaatcagc atgcctaata ttcctgcgac acttcctgct ccaatcaata ccaagatgat
     3841 cgtcaaagga tggatattca ttgatttccc aaataacaac ggcttgatca aatttccatc
     3901 taattgttgg atcactaaaa tcgacaacgc catatacaaa gcttgaaatg gagagataaa
     3961 caaaccgaca atgattgctg gtaccgctcc aataaaaggg ccgacatacg ggataatatt
     4021 cgtgattccg caaatgaccg caagcaacaa gctataaggc tgatcgaata ttctcattgc
     4081 caaaaagctc attacaccaa taatcaaagc atctaagatc gtactgctga tataggcaga
     4141 taatgtttcg tttaattctt ttaccgtttg gcgtaactct ttacgaatcg ctttcggaaa
     4201 gaagtgagac aacgcatcaa taaatttgtg cccatccttg aacataaata gcaaaataaa
     4261 tggaacggta aataacaaaa taaaaaattg catcataaat gaaatgattt ttgagacact
     4321 tgcagataaa ctgactaata tgatattgaa caaattacta agagataaat ttgctgatgc
     4381 taattgttct tccaaattga aatttttaaa ctcttcccgc tgcgccaatt catttaacca
     4441 tttgcttgtt tcatctgcat acattggcaa gctctgggtc aattgaattg tctgttccat
     4501 taactgaggc actacattca tgacagttaa aacaactaaa atgattacga ccaaaaacgt
     4561 caataaaaaa ccgaacactc tcgggatccg atgcttttcg agaaaaacaa cgattggatc
     4621 gaacatataa tagataaaac cagcaacaag caatggtaaa aaaactgccg aaagcatttt
     4681 caatattgga ctaaaaatat tcggcatttg taaaataaag tacacgccga tgatcgtgag
     4741 tactagctcc actgtccaga aaaataattt tgattgttta aaacgtgatt gcatacatct
     4801 cctccaaggt cctttgatac ttctattcta tcatttaaca aagagttacg cgctgagatt
     4861 ttcatttgct taaaaaaaac aaaaaaaata aacatatttt aatgagtaga cgagtactta
     4921 aacaaaaaca cggtttggag tagtgacaaa aattatactg cagtcccttt ttccaaataa
     4981 acggtgttca aaagcttgca tcttcgtaat aagcccagcc aaacgaaaaa tataaggaac
     5041 tatttttatt tggctgggct tattacttga tgtaggacat ctattttaca acctcagatc
     5101 atttattgcg caatactgac taaatgcaac tttgtgatat ttttgtgtac ggacaactcc
     5161 tcgatcacat cggcataagt gagccctttt ggaagatcaa tcgtatagat atttttgtac
     5221 actcggtagt catcaacgaa ttttacgtca aaattcacat cttcgattac gatatttttc
     5281 tcttcaaaat aatcggtaag aaaatctttg gtttcttttc tatggataaa ttggatttct
     5341 aactttttag tcgtaggtac atggatcacg cgtttcacta ctgtgagtgc aatagcaatc
     5401 ccgataaaac tgatgatcgc aatggaatag taacccatgc cgatggcgat ccctaatcca
     5461 gcaattgccc ataaagaagc ggcagtcgta agtcccgtca ccgattgttt cgtgacgatg
     5521 atcgttcccg cgcctaagaa accgacacca ctcaccactt gagcaatcag ccttgcttca
     5581 tctgaacgaa tcgttccagc aagctgtgga tactctaaag catctcttaa tgcaccacta
     5641 gcgatctcaa cttggatcaa tgcaatgata catgctccca tacaaactaa aatatgcgta
     5701 cgcatacctg ctggacgatt tttatattgt cgttcaaaac cgattgctcc gccaaatagc
     5761 atagcgatcc ctaaacgtaa aataatctct ggaattgtca ataccatatc attcatctaa
     5821 acaccctcca gtccaatata tcatgtctga aaggagcaga caaaaaaagt gcaattttta
     5881 cctcttacga aaaaaggact gtgacaaaat ttttgtcata gtccttttta tgaataaacg
     5941 gtgttcaatc agctgaccct cggtattact caacaaacgg caaaatatgg ggagctattt
     6001 ttcctttgtc ttctctaata ctcaggtcaa aacgctgatt tcacaacctc tttagcctac
     6061 tattgaaaat tacaacttac ttgctgcttc ttcatcgatg atcacaacaa cattgtcgtg
     6121 attttgcaat acacttgctg gcaaatgatt cgttacaggt ccttcaatca tccctttgat
     6181 tgcatcagct ttgctttcgc caaatgccat caagacgatt tctttacttt tcataattga
     6241 accgattccc attgaaacag cttttgttgg tacatcttct gctttatcga agaatcgttt
     6301 gtttgcattg attgttgatt ctgttaagtc aactactgaa gtcgttccgt caaatgaagc
     6361 accaggttca ttgaaaccga tatgtccatt ttgcccgatt cctaagattt ggatatcgat
     6421 tggatgtgca tcgatgattt gatcatactt tttgcatgct gcttccagat cttctgcttt
     6481 tccatctggt acaaacgttt ctttgaatgg ttttttatca aataattgga catccatgaa
     6541 atgacggtag ctttgttcat ctgaaccgcc tagtccaacg tactcatcta agtttacaga
     6601 agtcatttca ctgaaatcca aatcgcttga agtcatttct ttatataaag taactggtgt
     6661 gcttcctgtt gccaatccta atacttttgc acctttgttc ataccttctt tgatgatttc
     6721 aaatgctttt tttccgcctt cttcagcgtt ttttacacga atgatttcca tagtgattgc
     6781 tcctttatat tttggtatag cccaattata gcattctttt tttcttttta caataattag
     6841 tatagaccaa attgcatttt tttcattttc ttgacaataa ataggaatcc gtgttaggtt
     6901 tcataagatg gttcaatcaa acaatccatg ttgcatgaaa accaagcaag tgattcactg
     6961 tggacgcttt atgtctgcag tttttttatg aaaaaggagt tattatgagt atactgacat
     7021 taaacaacat tacccagcaa tttggcgaca aaatcctcta tgaaggtgtc gatctgcaat
     7081 taaatgctgg cgaacatctt gggctgattg gacaaaacgg agccggtaaa agtacattga
     7141 ttaaaatcat cactggtgag atcttaccag atgacggaca gatccattgg caaaaaaata
     7201 ttcacaaagg ttacctcgat caatatgtgg aagtcgatga aacattaaca atcgaagaat
     7261 ttctaaaaac tgcgtacgca aaagaatacg aaaaggaaga aaaaattgct tttctttacc
     7321 aaacatatag cgagaccttc gatgattcct tgctggaaaa agctgggaaa ctccaaacgg
     7381 aattagacca aggagtgttt tatcaaatcg acaccttagt tgctgagatg agcagcgggt
     7441 tggggatcga tgtcttaggt ttagattcac tgttgaaaaa tctaagtggt ggacaacgtt
     7501 caaaggtgat tttagcaaaa ctacttttag aaaacccaga tgttctgatc ttagatgaac
     7561 caaccaatca cttagatgat cagcatatcc aatggctgac gcagtttctt caggattttg
     7621 acggtgcgta tatcgtgatt tcccatgacc aagaatttct tgatcagatc accacccata
     7681 tcgcagacat cgagtttgga aagattacta agtatactgg ccatctaaaa caagcattga
     7741 aacaaaagga acaaaatcgc gaaagctatt tgcgccaata tcatgctcaa caaaaacaaa
     7801 tcgaaaagac cgaagcttat attcgaaaat acaaagccgg ttcccgatca acgatggcaa
     7861 aaagccgaca aaaacaatta gataaaatcg aacggctgac gcctccagct tcagcaagta
     7921 aagctgtctt tgattttcca ttctcgccta ttgtcacgac attagcagtg gaaacaactg
     7981 atttagtcat cggatatgat cgaccgctac ttgcacccat caatttgacc atccgctttg
     8041 gtgaaaatat tgccattcga ggcttcaacg ggattggaaa atctacttta ttaaaaacac
     8101 tgatcggtga gattgaaaaa ataagtggcg atttccactt cccagataac acaaaaatca
     8161 attacttttc acaagatctg tactgggaaa atccattaga aacaccacta caatatttaa
     8221 gcagtcaatt tccaaaagcg actatcaaag aattacgtcg ccaactagct aaagcagggt
     8281 tagttaatca attagcaagt gaacctctca ctacattaag tggtggggaa caaactaagg
     8341 tgaaacttgc acagttgaca atgaaccaag gtaatttact gattctcgat gaaccgacga
     8401 accatatcga tcaagaaacg aaagaaagct tacaagacgg gatacagaaa tttccaggaa
     8461 cagtaatcat cgtctctcat gaacaagaat tttatcaaga tcttgtgcaa cgagtcattg
     8521 agattgaacc aaaataaacg tttgattgaa tagttgaaaa aaaggagctg tgacaaaagt
     8581 tttgtcacaa ctccttcttc tgaataaatg gtggccaatt agctgacctt cggtattagc
     8641 tcaaaaaacg gcaaaatatg aggagctatt tttatttggc tattcttatt acttgatgca
     8701 gaacagcttt tcacaacctc ttttcgtcta ttttttattt tcgagatttc tctttccttt
     8761 aaaaacaact gtttgaaaga agaacgcaca tccttgaaaa acaagcccgc taaccgttac
     8821 acctaaccag ccatactgct gccacattgt cgtccctagc aatgaaccag ccgcacctcc
     8881 aataaagtac ataaacataa atacagtatt atttcggttg cttgctgctt cacctagccc
     8941 ttgtactcgt gtttgattgg cgacttgacc aaattgattc ccgacgtcta ataaaataat
     9001 agctaaaagc aaaatggcca aatggcttcc gccaaccaat aaagcaataa agcttattgt
     9061 ttgcataaca agtcccatat acacgatttt tctttcagaa taagaatctg ccagtcgtcc
     9121 cacgataggt gcagcaactg ctccagacaa accaaaaatc gctaatatcc ctgcttcgaa
     9181 tgttccccaa ttgtaggcgg gactgctgat ataaaaaatc aacgtagacc aaaagatcga
     9241 aaatgttcca aacatgaaaa atccactgac tgccgcttct cttaatagac gttgagaagc
     9301 aactaaatgc ggcaaacttt taagtgaatc aaggtatgaa acagctgtac tactttgctg
     9361 gacagaacgc ggcattttcc agtaaagcag aagtgtaaga attccgactg cacataaagc
     9421 aaataaatag atcatccgcc aagaaaaaag actggcaaca ataccagata ccgtccgaga
     9481 aagcaaaatc cccacgagta aaccgctaag caatgtaccc atgaccttcc ctcttgcttt
     9541 tggtcccgct agtaccgcac cgtaaggaat gatgatttgc ggcacgatcg aaagtaatcc
     9601 tattaaaaaa gaagcgcatg caaataacgt aaacgaaggc gcaaaaaaag cggccaacag
     9661 agagacggca gataacgctg ccattcgaat gatcaatttt ggacgatcca ccacgtcccc
     9721 taaaggaact aaaaacagta accctaacgc atagcctaat tgggtcaaca tcgtcaatat
     9781 cccgactgca cttgtgctaa cagataaact agctgctact tttgttccaa tcggctggat
     9841 gtaatacata ttggcaacaa caacgccaca agtgattgat aatagtaaaa taagcgaagt
     9901 tgttaaaggt tttttttcat tactcttcat tgatttcccc catttcctat cttagtttac
     9961 aacttatgga aaagattgga aatagaaaaa tacaactatt cgccacactt attatttgtg
    10021 cttttgtttt ccttgttttt tcttgcgatc tttataaatg aagacaacta accctactag
    10081 cacagtaaca cttgcaacca acgtgactgc agttttgtta tatccgtgaa acagagcttc
    10141 tccaccacac gcatacaaca cagacgaagg gatgactcca atcacgatcg acaatatgat
    10201 ttgctgtaga ggcaatttca tcgcatctgc agcaaaattg accatagaag atggaataac
    10261 cggtatcata tagcctaaca ttacacctat ttttggatgt ttcatttgtc ggattgcttg
    10321 cacccaatga ttcgtttcgg ttttcttatc taaaaatttt aagcgatgca taaggaaaat
    10381 tgcagtgatg ttaccaagag cattacccaa aatgttgatg atcgcacctg tcagtggtcc
    10441 aaagctcaaa ccagcaatga ctccaactac tgaagtagga atacctggaa cagcacacat
    10501 caccgctgtc aatacgatca agagtatcga tgcaaaagtc ccatgcgaac gaatatgtgc
    10561 gagtagctct gccttactcg ctctcggatt taaaaataat tggatgtctg tctgatattc
    10621 taaatacaac cgatagccca ctaccagtat cagaatgatt cccaacgaga tcaatatcaa
    10681 tcggattatt ttctttttca tatttcctcc tgaaaaaaat ctatattcat taaataatag
    10741 tttaacataa aaacaaaaaa gcaccgaaat ccaacatagg atctcagtgc ttttaaatag
    10801 taaaaattat ttaacagtta cagatgcgcc aacttcttca agtttagctt tcaattcttc
    10861 agcttcttct ttagatacgc cttctttaac tggtgcagga gcgccatcaa ctacagcttt
    10921 agcttctttc aagcctaaac cagttgcttc acgaactgct ttgataactt taactttttg
    10981 gtctccagct gcagttaatt ctacagtgaa ttctgtttgt tcttcagcag cagcagcgcc
    11041 accagcagca gctacagcta caggagcagc agcagatacg ccaaattctt cttcaatagc
    11101 tttaactagg tcgttaagtt ctaggatagt tgcttctttt aactcagcaa cgatgttttc
    11161 aatgttcaat gccattataa tttcctccgt ttaattgttc ttttttattt aatgctttat
    11221 gcggcaatta agctgcatct ccatcttctt tgtctgccac tgctttgaca gcgtaagcca
    11281 cgttgcggac tggcgcttgt agtacagata gcaacataga tagaagtcct tcgcgatttg
    11341 gcaattttgc caatgctgtg atttcttcca atgaagagac tttaccttcg atgataccgc
    11401 ctttgatttc taatgctttt gcttctttag caaattcgtc catgattttc gctggtgcga
    11461 ctacatcttc gttactgaat gcaacagctg taggtccagt gaatacttcg tccatgcctt
    11521 caaggccagc ttttttagct gcacgtgaaa gaatagaatt tttgataact ttcatttcta
    11581 cgttagcatc acgaagttgt ttacgtaaac gtgttacttc atcaactgtt aatccgcggt
    11641 agtcaacgac tactactgat gcagctgctg taaatttttc gcttacttca tcaacgattg
    11701 ctgctttttt tgcgataatt gcttcactca tttttgagtt tcacctcctg aattttgatg
    11761 gaagagtttt ttgcactgaa aaggcaagtc acttttatag ttactttgct caaccagtat
    11821 tattcatcac ctattcactt cgttcatagt gattctactc gctttgctcg aaaggaaact
    11881 cctttcgctt tgctcaacca gtattgttca tcatctcttc acttcgttca tagtgattca
    11941 caataaaaaa ctctatgcca ccgcagacat agagggacta ttgaatatat tcaataaacg
    12001 tcctcggtag gaaattaagg cttgatgcca cctactgtct tcggtacaag ttaattatta
    12061 acctcaacta gcttagcatg ctaaactagt tgagtcaaga attatttttc agttggatca
    12121 atcaacattc taatatttaa ttagaaagaa gcttggtcaa catggatacc aggtccaaat
    12181 gttgttgtga tcgttacatt tttcatgtat tgacctttag ctgctgatgg tttagctttt
    12241 agcaatacat catggattgc tgcaaagttt tcaactaatt tttcatcgtc aaatgaaact
    12301 tttccgattg gtacatggat atttccagct ttgtccacac ggtaagttac tttaccagct
    12361 ttcacttcgt ttactgcttt tgttacgtcc attgtaactg taccagtttt agggtttggc
    12421 attaagcctt ttggtcctaa tactcgtcct agacgaccta cagtagccat catatcaggt
    12481 gttgcaacaa ctacatcaaa gtcaaaccat ccgttttgga ttttttgaac catgtcgtcg
    12541 tcacctacga aatctgcacc agctgcttca gcttcttttg ctttgtctcc tttagcaaaa
    12601 actaaaacag tttgtgtttt accagttccg tttggtaata caacagcacc acggatttgt
    12661 tggtctgctt ttttagggtc tacgttcaaa cggtatgcaa cttcaactgt tgcatcaaat
    12721 tttgcaatgt ttgtttcttt tgctaaagct actgcttcgg caacaggata aactttactt
    12781 gaatcaactt tttttaatgc atcttgcatt tttttgctct ttttagccat tttggttcct
    12841 ccttgattgt ggttataacg gttgtacctc ccacgtgttc tatatctttc aatatagagc
    12901 caactgagaa gctgctgtct tagggtgtat agattattct acagtgatcc ccatgcttcg
    12961 tgcagttcct tcaaccatgc gcattgctgc ttcaacgtta gctgcgttta ggtcttccat
    13021 ttttaattca gcgatttctc ttacttgatc gctagaaact ttcgcaactt tatttttgtt
    13081 tggttcgcct gaaccttttt cgattttagc tgcttttttc aatagaacag cagctggcgg
    13141 tgtttttgta acgaatgtga atgaacggtc ttcgtataca gaaataacaa ccggaatgat
    13201 caaacctgct tgatcagctg tacgagcgtt gaattctttt gtgaatccca tgatattgat
    13261 acccgcttga cctagtgcag gacctactgg tggagctgga gttgctttac ctgcaggaat
    13321 ttgcaattta acgatttttt ctactttctt tgccacgaga catacctcct taagtccgtg
    13381 atgtggttaa ttggagatgc atatttctcc tcccacgttt tatctgatct gaaaaaatcc
    13441 agacaaataa tgtgcaaaac aatgcacact ggtacagtat agcaaattta tttgtttttt
    13501 caagattttt ttctgctatt tttacatttt gcttgaataa acgctaaatt tattctaaaa
    13561 aaacaacaat ttttcgttct catttcttct tttatgttac acttgcgcta gcatggttca
    13621 acagatgaaa gcaaacaata acacggtttt ttagaccctt gtacaaagat tcctacataa
    13681 caaaatgagg tggagaaaat gaaagattta aggtatagaa gtgtctttga tattattgga
    13741 cctgtgatga ttggtccaag tagctctcac actgccggag ctgctagaat cgggaaaatc
    13801 atacgtagta tatttggcga acagcctgat tctgtagata tttatctata tgaatcattt
    13861 gcaaaaacct atcgggggca cggaacagat attgcattag ttggcggcct gttagggatg
    13921 gaacccgatg atgaacgttt agctgattct ttgaaattgg catatgaagc cggtatggaa
    13981 gtcttgtttg ttcctaagag tgaaaaagcc gaacatccaa actctgtaaa gatgcttcta
    14041 aaaaaaggaa cacgaaagct ttcagtcact ggcatttcga tcggcggtgg caatatccag
    14101 atttctgaat tgaatggatt caaaatttct ttaagcatgg gtactcctac cttgatcgtt
    14161 gtccaccaag atgttccagg tatgattgct aaagtgacta gtatactttc tgagaccaat
    14221 acgaatatcg gcactatgac agtgacacgg gaatcaaaag gtgaaaatgc cattatgata
    14281 attgaagtag atgatcctca agtagaagaa acggtaaaaa gactcaaaca gctgccaaat
    14341 atcgacagcg taaattattt cgattaagga gctaaaaaaa tgtttttatc gattgaagaa
    14401 ttagtagatc aagcaaaagc ttatccaagt gtagcagaat tgatgattgc agttgaaatg
    14461 gaaatgagtg gccgttcccg tagtcacatc atcgagatca tggaaaaaaa cctctcagta
    14521 atggaacaat caatcaacga gggaaaagaa ggtgttacct ccgtcacagg aattaccggt
    14581 ggtgatgcaa aaaaattaga tgattattta aatgaggcag actttttaag tggagaaaca
    14641 atcttaaaag ctgtacgcaa tgctgtagct gttaatgaag tcaatgcaaa aatggggttg
    14701 atttgtgcta caccgactgc cggaagcgcg ggtgttgttc ctggtgtgct gatggctgtc
    14761 cgagatcggc ttgatttgac acatgaacag caattggaat tcttatttac cgctggcgct
    14821 ttcgggttag tcatcgcaaa caacgcttct atcagtggtg cagaaggggg atgtcaagca
    14881 gaagttggtt ctgcaagtgc aatggcagca gcagctttgg tttgtgcaaa aaatgggact
    14941 cctcatcaag cagcacaagc agtagccatt acattgaaaa atatgatggg actgatttgc
    15001 gatcccgttg caggcttagt tgaagtcccg tgtgtcaaac gtaatgcttt aggctcttct
    15061 caagctttta tctctgctga tatggcatta gcgggtatcg aaagtgtcat tccgccggat
    15121 gaagtcgttg ctgcaatgta ccaagtcgga cgtcaaatgc cgtcgatttt caaagaaaca
    15181 gcagaaggtg gcttagctgt tacgccaact gctaaacgac ttgcggaaga gattttaaat
    15241 aagagggcgt gataaagctc cttaatttcg agtattaaga gattagttat cttcataaca
    15301 aaaaacgaag agaggactaa ttgcccttct cttcgttttt tattaatcca tcaatttcgt
    15361 attttttact tcatcgatcg tttttgttcc tgcaagttgc atcgtgatcg acaattcttt
    15421 gttcaggtgt tcaaaaacag atgttactcc ttctgcccca cctagattca acccataaat
    15481 cactggtcgg ccaatcgcaa ctaagtcagc accacttgcc agagctttga agacatgctc
    15541 tcctcgacgg acaccagagt cgaaaatcac tggtacacgt ttgttgacta cttcggcaat
    15601 ttttggcaat acttcaaagg acgcaggacc tccatctagt tgacgtccac catgattgga
    15661 tacccagata ccatctgccc ctgcagagat agcgattact gcatcttctg gcgattgtat
    15721 tccttttacg ataactggaa gatgggtgat ttctttgatt gttttgatgt catctggtgt
    15781 caagccttgt tttgcagctg cataaatttc tgcgattcct tttccttcgc cattaccact
    15841 tgcactttgt tcgctatacg cagctaagtt aggcattggt aatggaaatt ggaattgatt
    15901 gatgacgtct tcttcacggt aaccaccgag cgttgaatct gccgttaaaa tgatagcttt
    15961 agcccctgct tctactgctt tgttcaaaat gaactcattg aatctgtcgt ctttactcat
    16021 atataattgg aaaaactgcg gtgcgcctgg cgctgcatcg gctgcgtctt tgattgtcgt
    16081 atttgcataa gtgctgatgg aaaagattga tccagcagca gctactcctt tagctgtatc
    16141 agcttcccct ttttcatgtg ccaatccttg ggccgctgat ggcgcttgga taatcggtgt
    16201 atctaattca attccgaaaa cagatgtatg caaatcggct gagtcgattc cgcgtaatat
    16261 ccgtggcatg attttctttg tattaaaaga agtcgtattt tctttcatcg tccactcatc
    16321 ttcagaacca ccgcgaatat agccaaaagc tcctgcttcc atgcgctctt ttacacgacc
    16381 ttctagttag gcaatgttta caatatcgat tggatgttct tcattacttg tatgataagt
    16441 catagtagat ccttctttca tcttcttgat tacatataaa tcatactaaa cttacaaaaa
    16501 gtaagcaaac atttactctc agatactttt atcgctattc tttccaaata aatcgactta
    16561 caagctgatc tacttcttgg ttttcatgaa gcatactatg agaggcattt tcacctgtca
    16621 ctactttttc ctctatttca tttccgcgtt gcttcaacaa cgcatagacc gctaatgcac
    16681 tgtttaacgg tactgttcca tcactcaagt ctgtttcatc tagttgacca gcaatcaaga
    16741 aaaatcgagt ggttatagga atattaccga tcatttgctg gtaatcttga tagcggttag
    16801 aaacgacagc aggccccttt gctaactcat ctgtcagaga ctgcgcacta tcatcgacaa
    16861 aatcattaaa aggcgcgcca attgctgtta atgtctgaac ttgtggcaat gacgtatcct
    16921 gtccatacgt tcctaaatag cgtagtgcac taactcctcc catggaatgc cccactatgt
    16981 tgactttatc tatgccgtag ttttctttca gatacaaaag acaagttttg atccattctg
    17041 cttgattcca ctcgttattt ttattatctt caaacaatac ttggaccatc ggattgtctg
    17101 cttttccaga caaatctcct gttgcttgta tttgtccatc tgttcctact gttaaaacca
    17161 attcttttac tgcttgctga tttttttcca aacgccgcaa catcccttta aaggaatttt
    17221 ctgtcccgct gtagccgtgg aaaaaaagtg ttggaatagt ggttttttct atcactttac
    17281 tggattctgc cgttttttct ttatcagaaa cttcagtagt atttatcttc gtcgtctggc
    17341 aaccacttaa cgcaaacaaa ctgataagga ctaaagctgc tgcagttgca cgtttgcata
    17401 aatttttgat tggttctgtc atacacctct tcctttccat atagaaaaaa gaaagacttg
    17461 aaaggccaat catatgatcg acatttcaag tctctttatt aatcgatttt atctacttga
    17521 tcgaaatcaa gttctgtact ggtttcacgt ccaaacatat cgatgttgac tttcaatttt
    17581 tctttttctc cgtcaacttc tgttacttgt ccttctaatc cagcaaatgc gccttcgatg
    17641 attttcacga cttcgccaac ttctacttct agatcatttt gtcgcaggct cataccgata
    17701 gaacgcaaga tatgattgac ttcttctgga agtaatggtg ctggtttgct tccggctccg
    17761 tgtgaaccaa cgaatcctgt cacaccaggt gtgttgcgga caacatacca tgaagcgtct
    17821 gtcatgatca tttcaacaag tacgtatcct gggaatgttt tatgaacgat ttctttttct
    17881 ttcccatttt tgacttcagt ttctttttct tcaggaacaa cgacacggaa aataaagtcg
    17941 gccattttca tactttgcgc acgagattcg atatttgctt ttactttgtt ttcatatcct
    18001 gaatatgtat gcaagacata ccagtttttt tcaaaagatt ccattgaagg ctcctcattt
    18061 cattgtaaaa taaaaaaacc tcagtacacc cgaagttttt ctttcgcttc attatagcac
    18121 taaaagtacg ttgtggctag cgagttattt taaaatccag ccaaataagc tttggatggc
    18181 tgtatccatt acgtaaaaca tcaatccgaa aataatagaa gtttcgatga ctactaacgt
    18241 atctttacgg agttgttttc tgcttggcca agttacttgt ttgatttcat cttttacgct
    18301 ttttagaaat ttcatgagac aacctccctt gctactgttt atttcgtttc tttatgtaat
    18361 gtatattgat tacaatattt acaaaactta ttgatttcca aacgttcgcc gctttttcct
    18421 tcactgactg attttgtgta gttgcgagac ccacaaacgg aacaagcgag tgctgctttt
    18481 ttctttgcca tttacttctg cccccttgct gagaatcaga cttatacttc ataacagtat
    18541 cacgtaactt tttacttgtc aatttgtgaa ataaactatc tattgttttg gataaaatta
    18601 tgatatgatt aatttgaaat taagctaaaa tgagacaatt ggaggtatag cgatgctatt
    18661 aaaaacactt gtatataaga aaaaagatct gacaaccgtt acagagacta gtacattaga
    18721 agaagcatta aaaattttag aaacttccgg ataccgttgt gtgccgatct tagattcttc
    18781 aggtaatatt ttccgtggaa acatttacaa aatgcatatc tatcgtcata aagcaaatgg
    18841 cggtgatatg acgcttcctg tcactcatct tttgaaaaac gctacaaagt tcatttacct
    18901 ggatacttct ttcttcaaag tattctttac gatcaaagag cttccttata ttgctgtcct
    18961 tgacagtgaa aatcattttt atggtatttt gacacatagt acgttgctta atatgctctc
    19021 tcaatcttgg agcgtagaac aaggaagcta tgtactgaca attgcttcca caggaaagca
    19081 aggagatttg gctgctattg caaaaatcat ttcaaaacac agcagcattg ccagctgtat
    19141 tactcttgat atcgaaaaag acgaatacat tcgacgtaca ttgatcacgc taccggctaa
    19201 cactgaagcg aaagtatgtg aaacgatcgt caaccactta gagaaaaaga attataaagt
    19261 ggtagaaatt gaaaatttgc aaaaccaaga agcataatca aaaagcgaaa aaagaaaaca
    19321 gctaatctgc tttctttttt cgctttttat aattagacac gcttttcaaa atcaagcgcg
    19381 cgatctaaat aatacatcaa tggtgctgaa acagccatga cgataaactc acttaatgca
    19441 agcgtgccat aggttgtcca aaatgcttgg ccacctgttg gaactaacat ataagcaatc
    19501 agtcccatgc tgatggtaaa gaagatcgtg ttcaatacta aacgcatctt tacatttttc
    19561 actttgtttt gcaataaagc tgtcaggcct aaagaaagca atgtttggcc gccaccaaaa
    19621 accacatcca tgataccaaa accaaagaag gcattataga cgatgacccc gcctaaaact
    19681 ccccaaagca atttacggtt aaagacaact aaatgattca aactttctga aatacgaaac
    19741 tggatcggcc ctgaggatac aggcgcaact aaaatagtta acgctagata aagagccatg
    19801 atgatgccat ttacaacgat tacggataac cgttgtctgc ttttcgtttt ttgcattttc
    19861 tctcctcttt tctcctagtt tttttaacgc gggatggttg atgaaccgcg gttatgtgaa
    19921 acaaacaata attataacag acttttcatt tatttcaatc cgtcttatct gcttttaaaa
    19981 aagcaaaagc ttaaacaatg taaacgtttg ctaaaaatcg tgggatatgg tataaagtaa
    20041 gtgtaagggg tcataagaat tcccagtaaa gcagacgaag taggatctgc aagaacagaa
    20101 ttgttgtgaa acttaaataa ataaaaaagg atgtgggaat ttgagcaacc tcttactaga
    20161 gaacggtcta gcaagcagct gaagatctaa gtggacatgc cgaaccttgg aacttctcct
    20221 tacgtaagta atggatcatg cgcttgctag gcttttttgt gtttttcggt cttcggacct
    20281 ttttcatttt cctagacttc tgctataata accaagaatt aattttgtaa cagatttttt
    20341 tgaaaaaaca agtttctttc attcaaaaaa tagaaaaagc ttgctaatta ttggttaacc
    20401 agcaaacagg agtattaaaa tatcttgttt tattgaatag caggaggaaa tcatgaaatt
    20461 ccattatcat atattgatca atccatcggc aggcagcgga aatggctata aagtagcaga
    20521 aagaatttta ccggtattaa aaaataagca tattgactat acacttcatt atagtgaata
    20581 caaaggacac gatgctgaga ttgctgaaac tttggcaaaa gaaacattga ttccttggat
    20641 agaagaagaa cagaaaacaa tcgatacttt cccccttttg atcatcgtag gaggtgacgg
    20701 caccttacat caggtacttg atactttcta tcagatggaa gtagaattcc cagttgctta
    20761 tattccagca ggttctggag atgattttgc tcgaggggca ggccttccaa aaaatcccaa
    20821 aaaaggactt cagctgattt tagcagctca atcaccagaa aaagtccaca taatggctta
    20881 tgaagaaaaa ataagtgaaa aaaaaggaat agctgttaat aatttcggta taggattaga
    20941 tgcagctatc gtccatgcga caaatcattc ttccacaaaa aagcgattga acaaatacaa
    21001 cttaggatca ttttcgtatt tattctctat tctccgtgct ttattcactc aaaaaggatt
    21061 tccaattctg gtagatgcag gaggaaaaca attaagtttt tcaaacgcat ttttatgtac
    21121 agcaacgaag catccttttt ttggcggagg aatcgcgatt gttccaactg ctgatgcgtc
    21181 taaaccagtc attgattttg tcgtggtcga acgaatcaat ttctttaaaa tcttgtggct
    21241 cattctttta cttatacaga aaaaacaatt gaaatcgaaa tatttccatc actacacaac
    21301 aaacagatta cgtatcgttt caacaacacc gcaacatggt caagaagatg gagaagaaat
    21361 ggaaaaacga ccttttgata tcacaataac cacaactaaa cagttgtttt ggtgttaata
    21421 aaagaggttt tgtcagaagc tacatctgga acaccgttta ttcggaaaaa aggactgtga
    21481 cacgactttt gtcacagtcc tttttttact atgcagttaa ttctttgccc aattgggttg
    21541 ccatcgatag tttctttctt tacccaaaat aaattggatc attgaaaaca ccgcagcaaa
    21601 agcaactaag acacaaccat tgaaccaaaa agattctgca aatgtttgat tgtaactgag
    21661 tgtttcactg atcaagttat tattttgaac aaaccaatat gttggtgtga atgccgctat
    21721 tttgctaacc atttctggca agtattctgc tggtacaaac accccaccga taaagcaact
    21781 gcctgtgata aaaatatctt tgatcccacc gctggtttct gagtttttta ccaatgaagt
    21841 gatacaagcc gaaaagctaa tgatcggcaa taaaaacaag aaagtattta aaatatgata
    21901 ccataccgtt atatcccacg aggtgtggat cactaacaga accattagaa gtaagaagcc
    21961 actgaacaac agcagcgagt aactaatcaa actaaaggtt actttgcgat tcaagtttcg
    22021 tttggtaatg ggcgcactct ggtttcgttt actgatctct ttgcgattaa atgctagatt
    22081 gatgacgctg aaaccgctaa aaatggatag aaacaaccca taagacaaga aattaaaggt
    22141 tgtgcccgtc aacagctttt tcatccgctg actatatccc gcacttagct caacttctcc
    22201 agtaaccgag agtgccattt gtgtctgttc catagctgca tcgattgttt tatcttcttc
    22261 ttgaaacaga atcagtgttt gcaaaaaggt gttcacttgc tgtgtaacaa ctgttttaga
    22321 aaaggcatct gggcgactct gtatcttgat cgtcggcatc tttcctactt cgacttgttc
    22381 ggtaaagtct gcaggcagat gtaagataat gtttacacga ttaaaataaa gagcatcatc
    22441 gatttctttt tgtgaggttc catccaatgt aacgactgtt tccttgtcat tcaaatattt
    22501 acgaaggcca tcgactacag gcgactcttc ttcactaatc aaggcgattt tcgcatcttg
    22561 cagagaggta ttttcaacat caatattttg cgcaaaaaag aatgtggcaa caacagtgat
    22621 aattacacct atcagtaaat tcgatttatt tgctttaagc aatgtccaaa tcgttttata
    22681 aagcgtcata acgcacccct ctttccatcc aagcatttcc taacgcaaaa aggaaaataa
    22741 atccgatcaa ccatagaaga ttggtataaa aactagccaa atcagtgtag aaataaagtt
    22801 t
//
LOCUS       DACDKM010000036        21912 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20834_2016
            SAMN12640370-rid9891663.denovo.036, whole genome shotgun sequence.
ACCESSION   DACDKM010000036 DACDKM010000000
VERSION     DACDKM010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640370
            Sequence Read Archive: SRR10040891
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 21912)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21912)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742835.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742835.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:04:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,012
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 29 of 75
            Pseudo Genes (incomplete)         :: 33 of 75
            Pseudo Genes (internal stop)      :: 19 of 75
            Pseudo Genes (multiple problems)  :: 5 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21912
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640370-rid9891663.denovo.036"
                     /isolate="E20834_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(459..1088)
                     /gene="udk"
                     /locus_tag="IV334_001297"
     CDS             complement(459..1088)
                     /gene="udk"
                     /locus_tag="IV334_001297"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HAP8943377.1"
                     /translation="MTKSKPIIIGVTGGSGSGKTSVSRAIFNNFPDHSIMMLEQDSYY
                     KDQSHLSFEERLNTNYDHPFAFDNDLLIQHVGDLLNYKAIEKPVYDYVAHTRSQATII
                     QEPKEVIILEGILILEDERLRDLMDIKVYVDTDDDIRIIRRIKRDMEERGRTLDSVIE
                     QYLTVVKPMYHQFIEPTKRYADIIVPEGGENHVAIDLITTKVASFLNHK"
     gene            complement(1172..1867)
                     /locus_tag="IV334_001298"
     CDS             complement(1172..1867)
                     /locus_tag="IV334_001298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="noncanonical pyrimidine nucleotidase, YjjG
                     family"
                     /protein_id="HAP8943378.1"
                     /translation="MKYKTLLFDVDDTLLDFQLTEANALSALFAEQGITLTPEIETSY
                     KALNHHLWQEFEKGRLSREEIIGSRFGLLFQQFGKTVDSDQMEKRYRHYLNQGHDLVP
                     GSLEILKKVHPHADLYIVTNGVSHTQHRRLTDAGMLDYFKDIFVSEAVGAQKPMKEFF
                     DHVFSHIPNFDKKQTVIIGDSLTSDIKGGNMAGIDTIWFNRNKLPEIEEIQPTYRIDS
                     LEELYSLLEIEKD"
     gene            complement(1880..2596)
                     /locus_tag="IV334_001299"
     CDS             complement(1880..2596)
                     /locus_tag="IV334_001299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HAP8943379.1"
                     /translation="MRLDKLIEEKLETTRKEMKRLFALKQVMIDGTVEFRQNRNVDSL
                     LHQIEVAGEHLETNESYYLLNKPEKAVTAVSDPEKTTVIDLIASADRFQPLYPVGRLD
                     RDTTGLLLITSNGQLGYEMLLPEKKVSKTYQVVVNERVTPADVTAFENGIVFADGYQC
                     KPADLKILKADKHQSTVLLTIQEGKFHQVKKMFLAQGKKVTALKRLSMGPLELDEQLG
                     VGEYRSLTLAEMKKLSIYFK"
     gene            2926..3534
                     /locus_tag="IV334_001300"
     CDS             2926..3534
                     /locus_tag="IV334_001300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25/general stress protein
                     Ctc"
                     /protein_id="HAP8943380.1"
                     /translation="MSVSLEVSKREVRPRSLRNKLRHEGKVPAVVYGYKVESTPISFD
                     GLTLSKLLREHGANTVITMDIDGQKVNTLVHKIQTNTFTNHFEHAEFLSVNMSEETEV
                     ETEVVLVGESVGAKSGGVLAQNLYTVVVSATPDKLPERIEVDVTNLELGDSITVADLP
                     KNDDYKFVTDSEEQIAAVVAQSEEEESTGEGGEPEVIGSTEE"
     gene            3859..4182
                     /locus_tag="IV334_001301"
     CDS             3859..4182
                     /locus_tag="IV334_001301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF960 domain-containing protein"
                     /protein_id="HAP8943381.1"
                     /translation="MFESFDSSRNRYASVGVVSSLPDELIDSIWFIIDLDLKGVIPLE
                     NILTFDLIENRGKVTMHFSQAGSNVEMDIDLPFAYSNQFPAQVYAYDDGTRETILLPS
                     EIRQY"
     gene            4364..5734
                     /locus_tag="IV334_001302"
     CDS             4364..5734
                     /locus_tag="IV334_001302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HAP8943382.1"
                     /translation="MRDLTKGSPAKLILMFTVPLLIGNVFQQFYNMIDMIIVGQTLGK
                     NALAAVGATGSLTFLIIGFAQGLTAGLAIITAQRYGAKDYRGLKKSFAASVVISLVVT
                     VILTVLSLLFIRPMLQLMQTPPEILDQAQTFISIILLGIFASVSFNLLSNVIRALGDS
                     RTPLFFLIIAVIINVVLDLIFIIYFGMGVEGAAIATVIAQVSSSVLCLVYIKKKMPLL
                     QLRKKDFKFEKEEFAVHLNAALPMAFQSSIIAVGAIVLQAALNSLGTDVVAAQAAAGR
                     IDQFANQPMMSFGIAMATFTAQNYGAKEYGRILQGVRQTLLMSVGFSLLAGAIVIFFG
                     HFFVQLFVSPSETRVFELAQTYFNINGSLYWILAVLFILRYTLQGLGQSKIPTIAGMM
                     ELLMRSFAAIVLTGMWGYPGAAAASPLAWIGSVAVLLYSYMRSMKQLKHMGEEEHFTL
                     EHAEAR"
     gene            complement(5864..7165)
                     /locus_tag="IV334_001303"
     CDS             complement(5864..7165)
                     /locus_tag="IV334_001303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HAP8943383.1"
                     /translation="MVINFIILFLMIAITSFFVASEFALVKIRRSRLEQLEKENVKNA
                     KLALHVTHHLDNYLSASQLGITLTGLIIGWVGEGSVAALLEPVIGKLPFSSAISSTIS
                     VALGFILVTYIDVVIGELLPKSYSIVNTEKVVLFVVKPLHYFYKVMFPFIWVLNHSAA
                     GLGKLLGVRLVSEGEETLSQEELTLVALNSYEKGELTKEEYYYLENVFEFDDTLAKDI
                     QVDRTSMQVFEASETVKEAIKASIEQGHTRYPVILESKDNVLGYVTLPDLIKQSYKDD
                     QLTVEQLIEEPIVTTETIPIKKLLTIMRKKGKHIAILKDEYGGTSGLVTIEDILEEIV
                     GEIRDETDLDEALIAEQSDGSYIISGKLTLDDFQRYFHVEIPEFEETNFTTLAGFASS
                     RYKEIKAGTIIEIASFRFTVLEYQHAHIDYFKVESTERKTE"
     gene            complement(7519..9510)
                     /locus_tag="IV334_001304"
     CDS             complement(7519..9510)
                     /locus_tag="IV334_001304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium transporter Kup"
                     /protein_id="HAP8943384.1"
                     /translation="MATTHNTKKISMTGLLVAMGVVYGDIGTSPLYVMKSIVEDNGGL
                     SGINPDFVIGSVSLIFWTLTLLTTIKYVVIALNADNHGEGGIFSLYTLVRKGGKYLII
                     PAMIGGAALLADGVLTPAVTVTTAIEGLRGIPQFFDRFGNNQNVIVIITLVIIFVLFM
                     VQRFGTDIVGKAFGPIMFAWFTFLGVMGLMNFSQDWTVIRALNPYYAIQLLFSADNKL
                     GLFILGNIFLATTGAEALYSDLGHVGKRNIRLSWPYVKICLVINYFGQAAWLLNVYQN
                     PEAQQIKNLNPFFQMMPQSWIVIGVAFATVAAVIASQALITGSFTLVSEAIKLKLLPR
                     LKIMYPGNSIGQMYIPAVNLILWIACSLVVVTFRTSHHMEAAYGLSITVTMLMTTALL
                     YFYLIQNGHSRWLAYLVTFFFGAIEVVFFISSVVKFFRGGFVAVLIGLIILVVMFIWE
                     QGNIIRESSTEDVSLRDYIPQLRELRDDTSLPIYQTNVVFLVPDMAEDKVGRQFIYSI
                     LDKRPKRARVYWFVHVEVTDEPYTKEYQVDMMDTDFIVQVNLFLGFRVQQEINVYVRQ
                     VIHDLMKQGRLPKQPQRYSLTPGREVGDFQFVLIDEVVSNVTTLGKWQRQIMQAKLAI
                     KKIATSPETWFGLEYSEVKHETVPLIIGNTRKTWLKERL"
     gene            complement(9654..9920)
                     /locus_tag="IV334_001305"
     CDS             complement(9654..9920)
                     /locus_tag="IV334_001305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HAP8943385.1"
                     /translation="MLNFLWSLFIGGILGLIAGAILGKEVPGGVSGNIIIGFLGSWLG
                     EFLLGPLGPELGGFYLIPALLGAIFCLALYSFVARRIRERPKKE"
     gene            complement(10430..12556)
                     /gene="pnp"
                     /locus_tag="IV334_001306"
     CDS             complement(10430..12556)
                     /gene="pnp"
                     /locus_tag="IV334_001306"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HAP8943386.1"
                     /translation="MSEKQVFKTTWGGRPLQIEVGQLAKQANGAVLVRYGDTVVLSAA
                     VASKEAKDTDFFPLTINYEEKMYAAGKIPGGFIKREGRPSTEATLTARLIDRPIRPMF
                     AEGFRNEVQVTNIVMSVETDCSPAMAAMLGSSLALSISDIPFDGPIAGVEVGRVNGEY
                     VLNPTVEQAEQTDIELTVAGTKQAINMVESGAKEVSEEDMLGALLFGFDAIKELVAFQ
                     EEIVQAVGKEKMEVTLLQVDEVLKKEIFDASYATMKAAVMTEEKLAREDNIEQVKIDI
                     REAYAEKFAGHEDEDHLLKEVKQITEDLEKDVVRELITIDKIRPDGRKLDEIRPLSSE
                     VSLLPRVHGSGLFTRGQTQALSACTLAPLGEHQIIDGLGVEVSKRFIHHYNFPQFSVG
                     STGRAGSPGRREIGHGALGERALAQVIPSEEEFPYTIRLVAEVLESNGSSSQASICAG
                     TLALMDAGVPIKAPVAGIAMGLVSDGENYTILTDIQGLEDHLGDMDFKVAGTKDGITA
                     LQMDIKIQGITEQILTEALTQAKQARMEILEELTSTIAAPREELSQYAPKIEMIQIEP
                     AKIKDVIGKGGDTINGIIDETGVKIDIDQDGKVSIASADAEMIKKAIKIIEDLTKEVK
                     VGEVYLGKVVRIEKFGAFVNLIKGKDGLVHISQLANDRVNKVEDVVKLGDEILVKVTE
                     IDKQGRVNLSRKAMLNEDGSTKNEEK"
     gene            complement(12744..13013)
                     /gene="rpsO"
                     /locus_tag="IV334_001307"
     CDS             complement(12744..13013)
                     /gene="rpsO"
                     /locus_tag="IV334_001307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HAP8943387.1"
                     /translation="MAISKERKNEIINEYARHEGDTGSPEVQIAVLTEEINHLNEHAR
                     VHKKDHHSYRGLMKKVGHRRNLLAYLRKTDVQRYRELIKRLGLRR"
     gene            13264..13827
                     /locus_tag="IV334_001308"
     CDS             13264..13827
                     /locus_tag="IV334_001308"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HAP8943388.1"
                     /translation="MITMDDIIREGNPTLREVAKEVSLPLSEEDISLGKEMLEFLKNS
                     QDPIKAEELHLRGGVGLAAPQLDISKRIIAVHVPSPDPEADGPSLSTVMYNPKILSHS
                     VQDACLGEGEGCLSVDREVPGYVVRHAKITVSYYDMNGEKHKIRLKNYESIVVQHEID
                     HINGVMFYDHINDQNPFALKEGVLVIE"
     gene            complement(14080..14601)
                     /locus_tag="IV334_001309"
     CDS             complement(14080..14601)
                     /locus_tag="IV334_001309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription repressor NadR"
                     /protein_id="HAP8943389.1"
                     /translation="MIEGETRRKQIMQTLANTEKPVSASKFAKTFGVSRQIIVGDIAL
                     LRAAGKSIVATARGYILETEETNTGHISKIAVQHGKDQTEEELRLIVENGGEIIDVIV
                     EHPLYGELAGTLHIKTPQDIDSFMRRYQKSNATLLSELTGGIHLHTIRYPEKHMLKNI
                     KQKLAEAGILYEG"
     gene            complement(14604..15446)
                     /locus_tag="IV334_001310"
     CDS             complement(14604..15446)
                     /locus_tag="IV334_001310"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HAP8943390.1"
                     /translation="MTEFKTEKEKMIAGELYFANDPELVADRMFARSQSQIINQAESA
                     ELRSQLLKETFGRTGKKIYMEPVINFDYGYNIFVGENFYANFNCTFLDVSTIEIGDNC
                     MFAPNVQLYTATHPLHPVKRNSGLEYAKPIKIGNNVWLGGGVIVTPGVTLGDNVVVGA
                     GSVVTKSFPDNVVIAGNPARIIKTVEEELTEESLETLRHKIDMIDRELVALLEKRMDT
                     VTKIGQVKKEENQAVYDAKREQQVLDKVVSLLKNKGYKETITDTYVDLMKHSREYQNK
                     MKEE"
     gene            complement(15439..16164)
                     /locus_tag="IV334_001311"
     CDS             complement(15439..16164)
                     /locus_tag="IV334_001311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent protein deacylase"
                     /protein_id="HAP8943391.1"
                     /translation="MKQLTVDEAAKEILAYQQITFLTGAGVSTPSGVPDYRSLSGIYH
                     GMEQPEYLLSHQAMVNEPDKFYQFVKKIYHPEAKPNIIHLEIAHLAKEKNVWVVSQNI
                     DGLHAKAKSPQLVNFHGSLYQCHCRKCQQTVDWKEYLHSDKHQACGGQIRPDIVLYGE
                     GFQDGVMEQAAYAVSQAELIVIVGTSFQVHPFCDLVQFRQPSAKILVINQTPVYLAEP
                     YTFIQEDGAVVFKKIQELGADYD"
     gene            16340..16636
                     /locus_tag="IV334_001312"
     CDS             16340..16636
                     /locus_tag="IV334_001312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8943392.1"
                     /translation="MLPFFIYWGVILACIAWLALSVYFSVFYLVRKENGNLWAFALFN
                     VIAAIVLAITLAVYRTWGWGITQYSSLIYLILAIYGVVVLLQAILGREPKKAAA"
     gene            17204..18796
                     /locus_tag="IV334_001313"
     CDS             17204..18796
                     /locus_tag="IV334_001313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750719.1"
                     /note="The N-terminal region of this protein, as described
                     by TIGR01726, is a three transmembrane segment that
                     identifies a subfamily of ABC transporter permease
                     subunits, which specificities that include histidine,
                     arginine, glutamine, glutamate, L-cystine (sic), the
                     opines (in Agrobacterium) octopine and nopaline, etc;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HAP8943393.1"
                     /translation="MKKNMVSVAVLFAFVFSLFSVGSLANADEKTPSDEFRVGMEAGY
                     APFNWSQQTDENNALPIQGQNSYAGGYDVQIAKKVADGLGKKLVIVQTKWDGLAPALQ
                     SGKIDAIIAGMSPTAERRKEIDFTDPYYESQLVVVVQKDSKYAKAKSLADLSGAKITA
                     QLNTFHYTVIDQIPDVQKQQAMDNFSAMRTALASGMIDGYVSERPEGVTATSVNKDLE
                     MLEFSGADGFQTNPEDVQISVGMRKGDPDIQKVNQILSGISNDERTKIMDQAVKDQPA
                     ATDSEEKPGIISDFTTIWKQYGNMFLRGAGLTLFIALIGTVVGTTLGLLIGVFRSVPE
                     SGNALTRFFQKIGNALLSIYVEVFRGTPMMVQAMVIFYGLALAFGISLNRTIAALFIV
                     SLNTGAYMTEIVRGGIFAVDEGQFEAAQAIGMTHGQTMRKVVIPQVLRNILPATGNEF
                     VINIKDTAVLSVIGVADLFFQGNSASGANFQFFQTFTIVGIIYLVMTFTITRILRAVE
                     KKIDGPSAYTKLEEIDNSNLQE"
     gene            18809..19549
                     /locus_tag="IV334_001314"
     CDS             18809..19549
                     /locus_tag="IV334_001314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HAP8943394.1"
                     /translation="MNEKIIEIKHLRKSFGENEVLKDISVTVNKGEVVTIIGSSGSGK
                     STFLRCINLLEKPTGGEIIYNGENVLAPKYNLPKYRTNLGMVFQSFNLFNNMNVLENC
                     MSGQTTVLKRDKETAKKVAIENLEKVGMDRYIEAKPAQLSGGQKQRVAIARALSMNPD
                     VLLFDEPTSALDPEMVGEVLNTIKDLAHTGLTMLIVTHEMEFAKDVSDRVIFMDKGVI
                     AEEGTPEDIFVHPKEERTKEFLSRILNA"
     gene            19717..21066
                     /locus_tag="IV334_001315"
     CDS             19717..21066
                     /locus_tag="IV334_001315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAP8943395.1"
                     /translation="MNEEQELQRGLKNRHVQLISIGGAIGTGLFLGSGKSIQLAGPSI
                     LLAYLITGCICFFIMRALGELLLSNTNNHSFLDFVAEYLGKKAAFITGWTYWFCWVSI
                     AMADLTAIGMYVRYWAPGVPQWLPELIALGLLLCLNMVAVSLFGELEFWFALIKVVAI
                     IAFILVGAYMILTHYTTDIGTASITNLWSHGGFFPTGAKGFILAFQMVTFAFAGIELV
                     GLVAGETENPEKVLPEAINNIPIRIILFYLGSLFVIMAIYPWNSLNPDNSPFVEVFSE
                     IGITIAASLINLVVLSAAASACNSAIYSTGRMLRSLAQEGSAPKKFKKLTTHHVPGNA
                     LTFSTIVIFISVILNYVMPSEVFTLVSSIATTCFLFIWSMLVYTHMKYRKSILGKKAH
                     SFKMPLYPFSNYLVFLYMAFVCVVLFLGKDTRIALLLTPVWFLLLLLIYHMKYERKK"
     gene            complement(21194..21784)
                     /locus_tag="IV334_001316"
     CDS             complement(21194..21784)
                     /locus_tag="IV334_001316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1211 domain-containing protein"
                     /protein_id="HAP8943396.1"
                     /translation="MTKSRLEAFTDGVVAIVLRVIVLEIKIPNTSSFHSLWVIRNTLL
                     AYTISFIFVAVIWVNHHRIFQMAERINYRVVWSNIFLLFWLTLCPSVTSWVGRNPEAF
                     WPEMAYVLVYTMWSFSFGILMRQIMKANKPDSHVVRVLSKDRRSLISMMINLCLIAGV
                     FFVPLIGLFGRFFVSGIWIFSYKKADAYYQRLFPGK"
BASE COUNT         6295 a         4462 c         4158 g         6997 t
ORIGIN      
        1 gggggtgcga caaaagagct tcttcaaaaa aataagtcga aatctcacac aaattcgaga
       61 aataattttc gtaagtttct tcttatttat tgcatctgac cacttctgtt acaacctctc
      121 gttaaacggt gttcaaaagc ttgcatctac gataataagc ccaacccaac gaaaaacgtg
      181 aggagctgtt ttatttggat gctcttatcg cttgatgcaa agacagcttt tttacaacct
      241 ctacttcacg gtgctcaatc agctgacctt cggtattagc tcaaaaaacg gtaaaatata
      301 aacagctatt ttccgtttct tctttttaat acttaggtca agatgctgtt ttcacaacct
      361 cttgttataa ataaaaaaac agttggctgg tgactgttca aaaagcagaa tccttgcttt
      421 tcaaacggtc gcagtccgtt caactgtttc gctgcgcact atttatggtt taagaaacta
      481 gctacttttg ttgtaatcaa atcaatagca acgtggtttt ctccgccttc tggaacaatg
      541 atatccgcat agcgcttcgt tggttcgata aattgatgat acatcggttt aacaactgtc
      601 aaatattgtt cgatcacgga gtccaatgtg cgtccccgtt cttccatatc ccgtttgata
      661 cgacgaatga tacgaatgtc atcatctgta tctacgtata ctttgatatc catcaagtca
      721 cgcagacgtt cgtcttctaa aatcaggatt ccttctagaa tgatcacttc ttttggttcc
      781 tgaatgatcg ttgcctgact tcttgtatgc gctacatagt catacacggg tttttcaatg
      841 gctttatagt tcagtaagtc gcctacatgc tgaatcagca gatcattatc aaaggcaaat
      901 gggtgatcgt aatttgtgtt caatcgttct tcaaaactca aatggctctg atccttgtaa
      961 taagaatctt gttcaagcat catgatggaa tggtccggga agttgttgaa aatcgcacgg
     1021 ctgacacttg tctttccgct tccggatcca cccgtcacac caatgataat tggcttgctt
     1081 tttgtcattg actaaaacct ctttcgtatc cgttttttca ttctaatcta ttatagcgaa
     1141 aaaacacgaa aatgctatat ttttctgaaa attaatcctt ttcaatttcc aataatgaat
     1201 aaagttcctc taatgaatcg attcgataag taggctggat ttcttcgatt tctggtaatt
     1261 tatttctatt gaaccaaatc gtatcgatcc ctgccatatt tcctcctttg atatcagaag
     1321 tcaaagaatc accaatgatc acagtttgct ttttatcaaa attaggaatg tgggaaaaga
     1381 catgatcaaa aaattctttc atcggctttt gcgctccgac agcttctgag acaaagatat
     1441 ccttgaagta atcaagcatt cctgcatctg ttaatcgccg atgttgcgtg tgcgacacac
     1501 cgttcgtcac aatataaaga tcagcatgcg gatggacctt tttcaggatt tctagacttc
     1561 ctggaacaag atcatgacct tggttcaagt agtggcggta gcgtttttcc atttgatcac
     1621 tatctactgt tttcccaaat tgctggaaaa gtaacccaaa acgactgcct attatttctt
     1681 ctcgtgatag tctgcctttt tcaaactcct gccataaatg atgattcaaa gccttatagc
     1741 tggtttcgat ttctggtgtc aatgtgattc cttgttctgc aaacaaagca cttaaagcat
     1801 tggcttcagt taattgaaaa tctaacaatg tatcatctac atcaaacaat aaagttttat
     1861 atttcatctt tttgacctcc tatttaaaat aaatacttaa ctttttcatc tctgctaatg
     1921 tcaacgatcg atactcgcct actcctagct gttcatctaa ttccaatggt cccatgctta
     1981 gccgtttcaa tgcggtcact ttctttcctt gggctaagaa catctttttg acttgatgga
     2041 attttccttc ttggatcgtc aataaaaccg tactttggtg cttgtctgct ttgagtattt
     2101 ttaaatcagc cggcttacat tgatacccat cagcgaatac gattccattt tcaaatgctg
     2161 ttacgtctgc cggtgtcacc ctttcgttca caactacttg ataagtcttt gaaacctttt
     2221 tttctggaag tagcatttca tatcccaatt gtccattcga agtgatcagc aatagtcctg
     2281 ttgtatctct atctagtctt cccactggat ataatggttg gaaacgatcg gcacttgcaa
     2341 tcagatcgat tactgtcgtc ttttctggat ctgatacagc tgtaacagct ttttcaggtt
     2401 tattgagaag ataataggac tcatttgttt ctaagtgttc acctgctact tcgatttgat
     2461 gcagtaaact atctacattt cgattttgtc gaaattcgac tgtgccatct atcatcactt
     2521 gcttcaaagc aaacagccgc ttcatttcct tgcgtgtcgt ttctaatttt tcttctatta
     2581 atttatctaa acgcatgctg tttcctttct tattactact aattaattat accttgaata
     2641 ttcttgcttg caaatacatc atcatagtta ttccttgact taattaacag ttcacgttac
     2701 ggttaaatag taaagtgaat tggatttctc atacacagcg taatgacctg gtggagaaaa
     2761 gttgaatcct gactttctaa ggggagatgc gcgcatctct ccttttgcgc ttttttaagc
     2821 gccctgcctt cgatgatgtg cacgagatta gcctttagaa ttcacttaaa taaaatctgt
     2881 ccgttaccaa agcagacagt cacattttaa ggaggaattt tggttatgtc agtatcatta
     2941 gaagtaagta aaagagaagt acgtccccgt tcattaagaa acaaacttcg tcacgaaggg
     3001 aaagttccag ctgttgttta tgggtataaa gtagaaagta cccctatctc attcgatggt
     3061 ttgacactaa gcaaattgtt gcgtgaacat ggagcgaaca cagtcatcac gatggacatc
     3121 gatggacaaa aagtcaatac attggttcac aagatccaaa cgaacacttt taccaatcat
     3181 ttcgaacatg cagaatttct atctgtaaac atgtcggaag aaacagaagt cgaaacagaa
     3241 gtcgttcttg ttggcgaatc agtgggtgct aaatctggtg gagtgttagc tcaaaacttg
     3301 tatacagtag tcgtatctgc tacaccagac aaattgcctg aacgaattga agttgacgtg
     3361 acaaacttgg aattaggtga ttctattaca gtagccgatc ttccaaaaaa cgacgattac
     3421 aaattcgtga ccgacagtga agagcaaatc gctgctgtag tcgctcaaag tgaagaagaa
     3481 gaaagcacag gtgaaggtgg cgaaccagaa gtgatcggtt caacagaaga ataatactga
     3541 aacaatcagg tggtgttaga agcggcgggt cagcagcttt taacacgcct tttttatttc
     3601 taatcgttta ttttcatatg gggcttccac ttttgtaaat ggctttacaa acgaaaaatc
     3661 ctaatttacc aagcttttcc tcgcatactt ttatcgtacc tttataaaaa tttatacttt
     3721 gaaaaaaagt attcaattat tgttgcattt ttcctgattc tttattagaa tgaaccctcg
     3781 ttgaagtaag tatttctagt ttatcctagg attaattata aaaataaaac attcgttgat
     3841 tcgaaggagg ttaaacagat gtttgaaagt tttgatagca gccgcaaccg ttacgcttca
     3901 gttggtgtcg tatcaagttt gccagatgaa ttgattgaca gtatatggtt tattattgac
     3961 ctagatctta agggagtcat tccattagaa aatatattaa cctttgattt gatcgaaaat
     4021 cgtggaaaag tgacgatgca tttttctcaa gcaggaagca atgtggaaat ggatattgat
     4081 ttgccgtttg cctattcgaa ccaatttcct gctcaagtat acgcctatga tgacggtacg
     4141 agagaaacga tccttttacc aagtgagatt agacaatatt aaggtgatag ataaacgtgg
     4201 atgtccctta ttttacagac caggccaagt tgttgattct ttgactcaat tcttggcttc
     4261 tttttatgga gtttcataaa ttatcattga tttctaattg gtattctcag gataaacgct
     4321 atatactact aactaaattt taaagatata aggagtttaa aagatgcgcg atttaacaaa
     4381 aggaagcccc gctaaactga ttttgatgtt cactgttcct ttactgatcg gaaatgtttt
     4441 tcagcagttc tacaatatga tcgatatgat catcgtgggt cagacgctag gtaaaaatgc
     4501 gctggcagcg gttggtgcaa ctggaagcct gacatttttg atcattggct ttgcccaagg
     4561 tttaacagct ggtttagcca ttattaccgc acaacgttac ggtgcaaaag attatcgggg
     4621 tctgaagaaa agttttgctg ctagtgttgt cattagtctt gtcgtcactg tgattttaac
     4681 tgtcctaagt ctcctgttta ttcgtccgat gcttcaattg atgcaaactc ctcctgaaat
     4741 acttgatcaa gcacaaacat ttatttcaat tattttatta gggatctttg catcagtcag
     4801 tttcaatttg ctttctaatg tcattcgcgc acttggtgac agccgcacac cactattctt
     4861 tctgatcatt gcggtcatca tcaacgtcgt acttgatttg atctttatta tctattttgg
     4921 catgggtgtg gaaggtgcag ctattgctac agtaatcgca caagtttctt ccagcgtact
     4981 gtgtctagtg tatatcaaga aaaaaatgcc tttgttacag ttacgcaaaa aagatttcaa
     5041 atttgaaaaa gaagagtttg ctgtacacct aaatgctgct ttgccaatgg cgtttcaatc
     5101 ttcgatcatc gctgttggtg ctatcgtgtt acaagcagca ctaaatagtc tagggacaga
     5161 tgtcgtagct gctcaagccg ctgctggtcg tatcgaccaa tttgcaaatc agccgatgat
     5221 gtcatttggt attgcaatgg caactttcac tgcacaaaac tatggtgcga aagaatatgg
     5281 ccgaatcttg caaggggtac gtcaaacttt actgatgagt gtcggattta gtctcttagc
     5341 aggtgctatc gtcattttct tcggtcattt ctttgttcaa ttgtttgtta gcccatcaga
     5401 aacacgtgta tttgaattag cacaaaccta cttcaatatc aatgggtctc tttactggat
     5461 cttagcagtc ctgttcattt tacgctacac acttcaaggg cttggacaaa gcaagatccc
     5521 tacgatcgcc ggtatgatgg aattgcttat gcgttctttt gcagctatcg ttcttacagg
     5581 aatgtgggga tacccaggag cagcagcagc ttctccttta gcttggatcg gttctgttgc
     5641 cgtgttgctt tactcttata tgcgttcgat gaaacaattg aaacacatgg gtgaagagga
     5701 acatttcaca ttagaacatg cagaagcaag atagccttga ttaaaagaaa tcgttgcggc
     5761 taaaaatgaa aacttttatt gaaaatatct tcttcgttta ccgaaaacag gttttttaga
     5821 taacaaaaag gctgtgacaa aaaatgtcac agcctttttt cttttattct gtttttcttt
     5881 ccgtggactc tactttgaaa taatcgatat gtgcatgctg gtattctaat accgtgaaac
     5941 ggaatgatgc aatctcaatg attgttcctg cttttatttc tttatatcgg ctagaagcaa
     6001 agcccgcaag tgtagtaaaa ttcgtttctt caaactctgg aatctctaca tgaaaataac
     6061 gctggaaatc atctaatgtt agtttgccgg agatgatata agagccatct gactgttccg
     6121 ctatcaatgc ttcgtccaaa tctgtctcat ctcggatttc cccgacgatt tcttctaaaa
     6181 tatcttcaat cgtcactaag ccgcttgtcc ctccgtattc atcttttaag atagcaatat
     6241 gcttaccctt ttttcgcatg atcgtcaata atttttttat agggatcgtt tcagttgtta
     6301 cgatcggctc ttcaatcaac tgttctactg tcagctggtc atctttataa gattgcttga
     6361 tcaaatctgg caaggtgaca taacctaaaa cattgtcttt tgattctaag ataaccggat
     6421 agcgagtatg tccttgttca atcgaagctt ttatcgcttc tttgacggtt tctgaagctt
     6481 cgaatacttg catggacgta cggtctactt gtatatcttt tgccaaggta tcatcaaatt
     6541 caaacacgtt ttccaaataa taatattctt ctttggtcaa ctctcctttt tcataagaat
     6601 tcaatgcgac taatgtcagc tcttcttgag acaatgtctc ttctccttca gatacgagac
     6661 gcacacctaa tagcttccct agccctgcag ctgaatgatt caatacccaa ataaaaggaa
     6721 acattacttt ataaaaataa tgaagtggtt taactacgaa caagacgacc ttctccgtat
     6781 tgacgatact atagctctta ggaagcagtt cgccaataac cacatcgata tacgtaacta
     6841 aaataaatcc cagcgccaca gagattgtac tgctaatagc cgaactaaaa ggtagtttac
     6901 cgatcactgg ttctagtaaa gccgcgacag atccttcacc gacccacccg atgatcaaac
     6961 cggttagcgt aattcctaat tgactagctg ataaatagtt atctaaatgg tgtgtaacat
     7021 gaagagctag cttggcgttc tttacatttt ccttttctag ttgttccaat cttgatcgtc
     7081 tgattttcac taaagcaaat tcacttgcta caaaaaatga agtaattgca atcatcaaaa
     7141 ataaaataat gaaattgata accatattct cccgcctttc tctttttcta ttgtaccctt
     7201 tttctacaaa aagattgaaa ataatgcgta ttcatattta ggaataaaaa aggagatgcg
     7261 gcatttttca gccacatctc cttatcactt caaaatcatt gcgttacttg ttgctaaaca
     7321 gctttttcat gacctctgat aaaaacggta ttcaaaagct tgcatctacg gcattaagcc
     7381 caaacagatg aaaaacatga gaagctgttt ttatctgctc gttcttactg cttgttgcag
     7441 tacagctttt tcatgacctc tgataaacgg tgttcaagta gctgacattc gacattagtt
     7501 caggtcaaag cgctactttc acaaccgctc tttcaaccat gttttccgag tatttccaat
     7561 gataagcgga acagtttcat gttttacttc gctgtactct agcccaaacc atgtttctgg
     7621 cgaggtagcg atttttttga ttgccagttt tgcctgcatg atttgacgtt gccattttcc
     7681 tagagttgtc acattggaaa ctacttcatc aatcaggaca aattggaaat cgccaacttc
     7741 tcgccctggt gtcagagagt atctttgtgg ctgttttggc aaacggcctt gtttcatcaa
     7801 atcatggatg acttggcgaa catatacgtt gatttcctgt tggaccctga aacctaagaa
     7861 cagattcact tggacaataa aatcagtgtc catcatatct acttgatatt ctttcgtata
     7921 tggctcatcc gtcacttcca catgaacaaa ccaatagact cgtgctcttt taggccgttt
     7981 atctaaaatc gaatagatga actgtcggcc aactttatct tcagccatat caggaaccaa
     8041 gaagaccaca tttgtctgat agattggtaa agaagtatca tcacgtaatt ctctaagctg
     8101 cgggatataa tctctcaaag acacatcctc ggttgacgat tcgcgaataa tgttcccttg
     8161 ttcccaaata aacatcacga ctaagatgat caaaccaatc agaacggcta caaaaccgcc
     8221 tcggaagaat ttgacaacac ttgagataaa gaaaacgact tctatcgcac cgaagaaaaa
     8281 ggtaactaaa tatgcaagcc aacgagaatg cccattttgg ataagataaa agtaaagcag
     8341 tgctgttgtc atcaacatcg tcacagtgat cgatagaccg taagcagctt ccatatgatg
     8401 agacgttcgg aatgtgacaa ccactagaga acaggcgatc cataaaatca gattgactgc
     8461 tgggatatac atttgcccga tactgttccc aggatacatg atctttaacc gaggcagcag
     8521 tttcaacttg attgcttctg aaactagcgt aaacgaacct gtaatcaatg cttgcgaagc
     8581 aatgactgca gcgactgttg caaaagcaac gccgatcacg atccaacttt gaggcatcat
     8641 ttggaagaat ggattcaggt tcttgatttg ctgtgcttca gggttttgat aaacattcaa
     8701 caaccacgca gcctgcccaa aataattgat aaccaaacaa attttgacgt aaggccagct
     8761 cagacgaata tttcgtttgc cgacatgtcc aagatcggaa taaagcgcct ctgcacctgt
     8821 cgtagccagg aaaatatttc caagaatgaa caagcctaat ttgttatctg cactaaaaag
     8881 cagctggata gcataataag gattcagtgc acggataact gtccagtctt gactgaagtt
     8941 cattaatccc attacgccta aaaaagtaaa ccaagcgaac atgattggtc cgaatgcttt
     9001 tccgacaata tctgtaccaa agcgttggac catgaataaa acaaaaataa taactaaagt
     9061 gataatgacg atcacgttct ggttattacc gaaccggtca aaaaactgcg gaattcctcg
     9121 caagccttca atcgcagtcg ttaccgtcac agcaggggtc aataccccat ctgctagtaa
     9181 ggcagctcct ccgatcattg caggaatgat caaatatttc ccaccttttc ggacaagtgt
     9241 gtagagtgag aaaattccac cttcaccatg gttatctgca ttcaatgcaa tcacgacata
     9301 tttgattgtt gtcagtagag tcaatgtcca aaaaatcaat gagactgatc cgataacaaa
     9361 atcaggattg attcctgaca atccgccatt atcttcaaca atcgatttca taacgtaaag
     9421 cggacttgtc ccgatgtctc cgtagacgac tcccatcgct acaagcaggc cagtcattga
     9481 tatctttttg gtattgtgcg ttgttgccac gtttttccct catttcttgt tatatggata
     9541 tcctttaggt aagcttgaat gatagatgat attcttatcc atgacacccg ctttcctaaa
     9601 ttgcttctaa ttatccaagt aacacactga agtgtcaact tatttgaata aaattattct
     9661 ttctttggac gttctctgat acgtcttgcc acaaatgaat aaagagcaag gcaaaaaatt
     9721 gcacccaata atgctggtat caaataaaaa ccgccgagtt ccggtcccag tggtcctagt
     9781 aaaaattcgc cgagccaact acctaaaaaa ccgataatga tatttccaga tacgcctccc
     9841 gggacttcct ttcctaaaat tgcgccagct atcaaaccta atataccgcc gataaataac
     9901 gaccataaaa aattcagcat ctcctttccc tcctttatcc atatttctca cttttatttt
     9961 acgctttatt cagaaggact ccaaaaatat gcgccaagaa actccagaaa aaaagaagtt
    10021 gtaacaaaag tgttcagctt atttccgtag aagctgcttc tggaacaccg tgaatcagag
    10081 gttgtgaaat cagcggtttg acctgagtat tagagaaaca aacggaaaaa tagttcctca
    10141 tattttgccg ttttttgagc taatatcgag ggacagctga ttgaacatcg tttattggag
    10201 aggttgtaaa aaagctgttc tgcatcaagt aataagaaca gccaaataaa aacagctcct
    10261 aatgttttta acttggctgg acttattacc gcagatgcaa gcttttgaac accgtttatt
    10321 cgaaaaaaaa ggactgtgac acggcttttg tcacagtccc cttttttcga ataattattg
    10381 tccccctgtc accttcacga tcacaagtac attattccta tgaattaaat tatttttctt
    10441 catttttcgt tgaaccatct tcgttcaaca ttgcttttct tgacagattt acgcgtcctt
    10501 gtttatcgat ttcagttact ttcacaagga tctcatcacc tagtttaaca acatcttcga
    10561 ctttattgac acgatcgttt gctaattggg aaatatggac aagtccatct tttcctttga
    10621 tcaagttgac gaatgcaccg aatttttcga tacggacaac tttccctaga taaacttcac
    10681 caactttgac ttctttggtc aaatcttcga tgatcttgat agcttttttg atcatttctg
    10741 catcagctga agcaatactt acttttccat cttgatcgat atcgattttc acaccagttt
    10801 catcgatgat tccgttgatc gtatcgccgc ctttaccgat aacatctttg attttagcag
    10861 gttcgatctg gatcatttcg atttttggtg catattggct taattcttca cgtggagcag
    10921 caatcgttga agtcaattct tctaagattt ccatacgtgc ttgttttgct tgagttaatg
    10981 cttcagttag gatttgttct gtgattcctt gaatcttgat atccatctgt aaagcagtga
    11041 taccatcttt tgtaccggca actttaaagt ccatatcgcc aagatgatct tccagccctt
    11101 ggatatcagt taggatcgta tagttttcac catcagaaac aagtcccatc gcaataccgg
    11161 cgactggcgc tttgattggt acacctgcat ccatcaatgc taatgtgcca gcacagatac
    11221 ttgcttggga agaagaacca tttgattcca atacttctgc tactaaacgg atcgtataag
    11281 gaaattcttc ttctgaaggg attacttgtg ctaacgcacg ttcacctaat gcgccatgtc
    11341 cgatttcccg acgccctgga gagcctgcac gtcctgtaga accaacagaa aattgaggga
    11401 aattgtagtg gtggatgaat cgtttgctga cttccacccc taaaccatca atgatttggt
    11461 gctctcctag aggtgcgagt gtacaagctg ataaagcttg tgtttgtcct cgtgtgaaca
    11521 aacctgatcc atgtacacgt ggcaataagc tgacctctga tgacaatgga cggatttcat
    11581 ccaatttacg cccgtcagga cggatcttat cgatggtgat caattcacgt acgacatctt
    11641 tttcaagatc ttctgtgatt tgtttaactt ctttcaacaa gtgatcttca tcttcatgac
    11701 ctgcaaattt ctcagcataa gcttcgcgga tatcgatctt cacctgctcg atattatctt
    11761 cgcgagcaag tttttcttca gtcattactg ctgctttcat tgtagcgtaa gaagcatcaa
    11821 agatttcttt tttcaatact tcatcaactt gcagtaaagt cacttccatt ttttctttcc
    11881 ctactgcttg aacgatttct tcttggaaag caactaactc tttgatagcg tcaaaaccaa
    11941 acagcaatgc accaagcatg tcttcttctg aaacttcttt cgcgccgctt tcaaccatat
    12001 tgatggcttg tttcgttccg gcaacagtca attcgatatc tgtttgttct gcttgttcta
    12061 ctgttggatt caagacgtac tcgccattta cacgaccaac ttctacgcct gcgattgggc
    12121 cgtcaaatgg gatatctgag attgatagtg ctaaagaaga acctagcatt gctgccatag
    12181 ctggcgagca gtctgtttct acactcatca cgatgttcgt tacttggact tcattacgga
    12241 agccttctgc aaacatcggg cgaattggac ggtcgatcaa acgggcagtc aatgttgctt
    12301 ccgtacttgg acggccttct cgtttgatga aacctccagg gattttccct gctgcataca
    12361 ttttttcttc ataattgatc gttaatggga aaaagtctgt atcttttgct tctttagagg
    12421 ccacagctgc acttaagact actgtatctc cataacggac taaaacggct ccgtttgctt
    12481 gttttgctag ctgaccgact tcgatttgta agggacggcc gccccatgtc gttttgaata
    12541 cttgtttttc tgacataact tctccttttc cttctcgtga aagcaagacg ctactaaaca
    12601 aatgaaaact gccgaaagcc gtcttttgag cagctttcat ttggttagta ggagtccatc
    12661 tagcttgatc acggaatttt acttaattga tatacgagta aaaaaagtga gattcatgtg
    12721 aatcccactc tttttgacta aaattaacga cgcaagccta aacgtttgat caattcgcgg
    12781 taacgttgaa cgtcagtttt acgtaagtaa gctaacaagt tacggcggtg accaactttt
    12841 ttcattaggc cgcggtatga atgatggtct tttttgtgca cgcgtgcatg ttcattcaag
    12901 tggttgattt cttcagttaa tacagcgatt tgtacttctg gagaaccagt atccccttca
    12961 tggcgagcgt attcgttgat gatttcattc ttgcgttctt ttgaaattgc catttctttc
    13021 acctctttcg attattcccc tgcgactgag taaaacgttg gtgattcgct taaccaagtg
    13081 gcaggtcatg tgcctagagc acacagctat actttacctg aaaccgggtt gaaaatcaag
    13141 tagttaatca ttaagaaaag gtagttttca aacaatctta ctactacttt tccatttttg
    13201 tggtaaacta taatgcgaaa atagacttga aacacagtct tttaagaaag gggtacatat
    13261 tccatgatta caatggatga tatcattcgt gaaggaaatc cgaccttacg cgaagttgca
    13321 aaagaagttt ctcttccatt aagcgaagaa gatatctctt taggaaaaga gatgcttgag
    13381 ttcttaaaaa acagccaaga tccaatcaaa gctgaagaac tacacttacg aggcggtgtt
    13441 ggcttagctg ctcctcaatt agatatttct aaaagaatca ttgctgttca cgtaccaagt
    13501 cctgacccag aggcagatgg accttctcta agtactgtta tgtataatcc taaaattttg
    13561 agccattctg ttcaagatgc ttgtttaggg gaaggtgaag gctgtttatc tgttgatcga
    13621 gaagtaccag gatatgttgt tcgccatgcc aaaatcactg tgtcttatta tgatatgaat
    13681 ggtgaaaaac acaagattcg tttgaagaat tatgaatcga tcgtcgttca acatgaaatc
    13741 gaccatatca acggtgtgat gttctatgac cacatcaacg atcaaaatcc atttgcacta
    13801 aaagaaggcg tattggtgat cgagtagaga attggcaggt tccttcgata ctatgctaca
    13861 agaaagcttt ataaaaaagg ggagtgctca aaagctgcac ttcccttttt tatgtttctc
    13921 cactaatcga aatttagata gacatctaga aatcgcaatt ccaaaaaatt ctgtgtattg
    13981 gatacgcatc ttttcttatt ttttcttatt tcttccatat gggtgtttat ctcagagatg
    14041 atagaatagt ttctaggata aaaagtagca gctacgatgt tatccttcgt agaggattcc
    14101 tgcttcagct agtttttgtt tgatgttttt cagcatatgc ttttctgggt agcggatcgt
    14161 atgcagatgg atgccgcctg ttaattcgga taacaaagta gcattacttt tttgataacg
    14221 tctcataaag gaatcgatat cttgtggcgt tttgatgtgc aaagttcctg ctaactcacc
    14281 gtaaagggga tgttccacaa ttacatcaat gatttcacca ccattttcaa cgattagtct
    14341 cagttcttct tctgtttgat cttttccatg ctggacagcg attttactga tgtgcccagt
    14401 attggtttct tctgtttcga ggatataacc tcttgctgtt gccacgatac ttttacctgc
    14461 agctcgtagt aaagcaatat cgcctacaat gatctgacgg ctgactccaa aagttttcgc
    14521 aaacttgcta gcactcactg gtttttctgt atttgccaag gtttgcatga tttgttttcg
    14581 ccgtgtctct ccttcgatca cacttattcc tccttcattt tgttttggta ttcgcgcgag
    14641 tgtttcatca aatcaacata cgtgtctgtt attgtttcct tatatccctt atttttcagc
    14701 agactaacaa ctttatccaa tacttgctgc tctctttttg catcataaac tgcttggttt
    14761 tcttcttttt tcacttgtcc tattttagtt accgtatcca ttcttttttc taataaagca
    14821 accaactctc gatcgatcat atcgatctta tgacgtaatg tttcaagaga ctcttctgtt
    14881 agttcttcct ctactgtctt gatgatacga gcaggatttc ctgcaatcac tacattgtcg
    14941 ggaaaagatt tggtcacaac cgaacctgcg ccgactacta cgttgtcccc tagagtgaca
    15001 cccggcgtga cgataactcc tccgcctaac caaacattat taccgatttt aatgggtttt
    15061 gcgtactcca gaccgctgtt acgtttcact ggatgtagtg gatgcgtcgc cgtgtaaagc
    15121 tggacattcg gagcgaacat gcagttatca ccgatttcaa tcgtgctgac atctaaaaat
    15181 gtacaattga aatttgcata aaaattctcc ccaacaaata tattatatcc gtaatcaaaa
    15241 ttgataactg gctccatata gatttttttc cctgtacggc caaaagtctc tttcaacaat
    15301 tgggatcgca gttccgctga ttctgcttgg ttgatgattt ggctttgact tcttgcaaac
    15361 atacggtcgg caaccagctc aggatcattg gcaaaataaa gctcgcctgc aatcattttt
    15421 tctttttcgg ttttgaattc agtcataatc tgctcctaat tcttgtattt ttttgaaaac
    15481 aaccgctccg tcttcttgaa taaacgtata aggctccgct aaataaacag gcgtttgatt
    15541 gattactaag attttggctg acggctggcg aaactgtacc agatcacaaa acggatgaac
    15601 ttgaaaactc gttccaacaa tgacgatcag ctctgcttgg ctgacggcat aagccgcctg
    15661 ctccatgact ccatcttgaa acccttcacc atataacacg atgtctgggc gtatttgtcc
    15721 tccgcatgct tgatgtttgt cgctgtgaag atattctttc cagtctaccg tttgttgaca
    15781 ttttcgacaa tgacattgat acagactccc atgaaaattg acgagttgcg ggcttttagc
    15841 ctttgcgtgc aaaccgtcga tgttttggga aacaacccaa acattttttt ctttcgctag
    15901 atgtgctatt tccaaatgaa tgatattggg ttttgcttca ggatgataaa tctttttgac
    15961 aaattgatag aatttatccg gttcattcac cattgcttga tgactcagca agtattccgg
    16021 ctgctccatc ccgtggtaga ttcctgacaa agatcgatag tctggcactc cagaaggtgt
    16081 ggatacccct gcaccggtca aaaatgtaat ttgctgataa gctaatatct cttttgctgc
    16141 ttcgtctact gttaattgtt tcatgttttg tcacctcact ttccattata gcccatactc
    16201 gaacaaattt attgttgaaa agtcagaaaa atgggaataa aaaagtgtta tgggcatttg
    16261 agcaatttat cacaatgttc actgacaaaa gaatgaaaac gtgttacaat actgctgtat
    16321 aaaagtgagg tgaatcgata tgttaccatt ctttatttat tggggcgtta ttctggcttg
    16381 tatcgcctgg ttagcgttaa gcgtttattt ctctgttttt tatcttgtac gcaaagaaaa
    16441 tggaaatctt tgggcttttg ctttattcaa cgtgattgcc gcaattgttt tagccattac
    16501 attagccgta tatcgaacat ggggctgggg aatcacccaa tactcaagtt tgatttatct
    16561 aatattagcc atttatggcg tcgttgttct tttgcaagcg atcctaggac gtgagccaaa
    16621 gaaagcggca gcttaaatga tcgagatgga actggacaca ggaaattttc tgttctagtt
    16681 ctttttttat tgtattacat acatttttgt gtataattat actatcgttt gcaatggata
    16741 gatgattcac attttgattg ttttttcggt aaaagtaaag atttctatat ttatcagtat
    16801 tttttcaaaa accagcagtt atagattgac aaagcctctc gagtatcgta aacttttttt
    16861 gtataaataa ctgaatacga gatgcctcaa tcaatgaggt agaggtcgcg agaatcatta
    16921 accctgtgga gtgaagcaaa catggagaag caggaatagg gaggatcgcc gaaatgtgca
    16981 cttttttgct tggagtgtat gttgggacgc aagttaagag cttgcggact gtcttagcag
    17041 tgatgctaag ttgcgctatg ttttgtgaga ggtttatcac cttatggaat tttagcggcc
    17101 tttttatatc tataaatcgg tcgcttttta ttttttcaga taaagcttta ccgcatttca
    17161 tctagaccgc tcgtgtatca tataatcaga aagggagaaa aaaatgaaaa aaaatatggt
    17221 ttcagttgcc gtactatttg cttttgtctt ctcactattc agtgtgggat ctctggcaaa
    17281 tgcagatgaa aaaacaccga gcgatgagtt tcgcgtaggt atggaggctg ggtatgcacc
    17341 cttcaactgg tctcaacaaa cagatgagaa taatgccttg ccaattcaag gccaaaatag
    17401 ttacgccggc ggttatgatg ttcaaatcgc taaaaaagta gcagatggtt tagggaaaaa
    17461 attagtcatc gtccaaacga aatgggatgg tctggctcct gcccttcaat caggcaagat
    17521 cgacgcgatc atcgccggaa tgagccctac tgctgaacga cgaaaagaaa ttgattttac
    17581 cgatccttat tacgaatcac aattagtagt agtagttcaa aaagatagca aatatgcaaa
    17641 agcaaaaagt ttagcagatc tttctggagc aaagatcact gctcagttaa atacattcca
    17701 ctatactgtg atcgatcaga taccagatgt gcagaaacaa caagcaatgg ataatttctc
    17761 agcaatgaga acagctttag cttctggaat gatcgatggt tatgtcagcg aacgtccgga
    17821 aggtgtaacg gcaactagtg tgaacaaaga cttggaaatg cttgaattca gcggtgcaga
    17881 tggcttccag acaaacccgg aagatgttca gatttctgtt ggtatgcgta aaggcgaccc
    17941 ggatatccaa aaagtaaacc agattctttc tggcatctct aacgatgaac gaaccaaaat
    18001 tatggaccaa gcagtcaaag atcagcctgc tgcaactgat agtgaagaaa aacctggcat
    18061 catttctgat ttcactacga tttggaaaca atacggcaat atgttcttac gaggtgctgg
    18121 acttaccttg ttcatcgcat tgatcgggac agttgtcggt actacacttg gattactgat
    18181 tggtgtcttc cgttcggtac ctgaatcagg aaatgcactg actcgcttct tccaaaaaat
    18241 cggaaatgca cttctttcta tctatgtcga agtattccgc ggaacaccga tgatggtaca
    18301 agcaatggtc atcttctacg gtctggcact cgcttttggc atttcattga accgaacgat
    18361 tgccgctttg tttattgtct ctctcaatac tggagcttac atgacagaga tcgttcgtgg
    18421 ggggattttt gcagtagatg aagggcagtt cgaagctgcg caagccattg gtatgaccca
    18481 tggccaaacg atgagaaaag ttgttattcc acaagtttta cggaatatct tgccagcaac
    18541 aggaaatgag tttgttatca atatcaaaga tacagcagtt ttaagtgtaa tcggcgtagc
    18601 tgatttgttc ttccaaggaa actctgcttc cggtgcgaat ttccaattct tccaaacatt
    18661 tacgatcgtc gggatcatct acttagtcat gacctttacg atcaccagaa tcctgcgggc
    18721 ggtagaaaag aaaatcgatg gtccttcagc ttataccaag cttgaagaaa tcgacaattc
    18781 aaatttgcag gaataaggag gaagaatcat gaatgaaaaa attattgaaa tcaaacatct
    18841 aagaaaaagc ttcggtgaaa atgaagtatt gaaagatatc agcgtaacag tcaataaagg
    18901 tgaagttgtg acgatcatcg gttcttccgg ttctggtaaa tctactttct tgcgctgtat
    18961 caatttactg gaaaaaccaa caggcggcga gatcatttat aatggagaaa atgtattagc
    19021 tcctaaatat aatttaccta aatatcgaac gaatctaggt atggttttcc aatccttcaa
    19081 cctgttcaac aatatgaatg tcttggaaaa ctgtatgtct ggtcaaacaa ctgtcctgaa
    19141 acgagataaa gaaactgcca aaaaagtagc aatcgaaaat ctggaaaaag ttggtatgga
    19201 tcgttatatc gaagccaaac cagcacaatt atctggtgga cagaaacagc gtgtcgcaat
    19261 tgctcgggca ttatcaatga atccagatgt attgctattc gacgaaccta cttcggcgtt
    19321 agaccctgaa atggttggcg aagtgcttaa tacgatcaaa gacttagcac atactggctt
    19381 gacaatgctt atcgtgacgc atgagatgga atttgcaaaa gatgtatctg accgtgtcat
    19441 ttttatggat aaaggtgtga ttgcagaaga aggtacacca gaagatatct ttgtccatcc
    19501 gaaagaagaa cgtacaaaag aattcctttc acgtatttta aatgcttagg atcaatagct
    19561 gattgggaca aagagctgcg gtgtttaccg cagttctttt ttatgaatta ctcataaaag
    19621 gtaaatttct gaaaattttg gaatcttagt tgtgtttttc agaaaactct cttaatatgg
    19681 aagtaattaa acttttttag ttaggatgat ttaaaaatga atgaagagca agaactgcag
    19741 cgtggactga aaaatagaca tgtccaactg atttctattg gcggagcgat tggaacgggg
    19801 ttgtttttag gttccggaaa gtctatccag ctagctggcc cttcgatatt gctcgcctat
    19861 cttattactg gatgcatctg ttttttcatt atgcgtgcgt taggcgaact tctgttatca
    19921 aacaccaata accattcttt tctggatttt gttgcagaat atcttggcaa aaaagcggct
    19981 ttcatcactg gctggacgta ttggttctgc tgggtatcga ttgcaatggc cgatttaaca
    20041 gccatcggaa tgtatgtacg ctactgggcg cctggtgttc ctcagtggct tcctgaactg
    20101 attgcattag gtcttcttct ttgcttgaat atggtggctg tctcattgtt tggtgagtta
    20161 gagttttggt ttgcattgat caaagttgta gcgatcattg cctttatcct cgtaggagct
    20221 tatatgatcc tcacccacta tacgacagat attggcacag ccagtatcac gaatctatgg
    20281 tctcatggcg gattctttcc aactggtgca aaaggattta tcttagcttt ccaaatggta
    20341 acctttgctt ttgctgggat agaattagtt ggattagtcg ctggagaaac agaaaatcct
    20401 gaaaaagtgt tgccggaagc tatcaataat attcctatca ggatcatctt attttactta
    20461 ggatctcttt ttgtcattat ggcaatctat ccatggaaca gcttgaatcc agataacagt
    20521 ccatttgtag aagtattttc ggaaattggg atcacgattg ctgcttcctt gatcaattta
    20581 gtcgtattaa gtgcagcagc ttctgcctgc aacagtgcaa tatatagtac aggacgtatg
    20641 cttcgttctt tagcgcaaga aggcagtgcc cctaaaaaat tcaaaaagtt aacaacacat
    20701 catgtgccag ggaatgcttt gacattttct actatcgtca ttttcatttc tgttatcttg
    20761 aattacgtga tgcctagtga agtattcaca ctagtaagca gtattgccac tacttgtttc
    20821 ttgtttatct ggagtatgct tgtctacacg cacatgaaat atcgtaaaag tatcctcggc
    20881 aaaaaagctc attcatttaa aatgccgttg tacccattta gtaattatct ggtatttctt
    20941 tacatggcct ttgtgtgtgt cgtcttgttc ctaggaaaag atacgagaat cgctttatta
    21001 ctaacacctg tgtggttctt gcttctatta ctaatctatc atatgaaata tgaacgtaag
    21061 aagtaagaaa gtagttgtga cagaagtggt cagcttcgag aaataagaag gaatttacga
    21121 aaattgcttc ttaaattttt gtgaaatttc ggcttatttc ttggagataa aagcttctgt
    21181 cccagcctct tttctatttc cccggaaaca accgctgata ataggcatct gcttttttat
    21241 aggaaaaaat ccaaatacca gatacaaaaa aacgtccgaa taacccgatc aacggtacaa
    21301 aaaagacacc ggcaatcaag cataagttaa tcatcatgga gataagactt cgacggtcct
    21361 ttgacaaaac tctcacgaca tgggaatctg gtttatttgc tttcatgatt tgcctcatca
    21421 agataccaaa agaaaaactc cacatcgtgt atactaagac ataagccatc tcaggccaaa
    21481 aagcttcagg gttacgcccg acccaactag taacagaagg acataaagtc agccaaaaga
    21541 gcaaaaagat attcgaccag accactcggt aattaattcg ttctgccatc tgaaatatac
    21601 gatgatggtt cacccagatc accgcaacaa atataaagct gatggtataa gctaataatg
    21661 tatttcggat gacccataaa gaatgaaagc tagatgtatt agggatcttg atctctaata
    21721 caataactct caaaacaatg gctaccactc catcagtaaa tgcttctaat ctcgactttg
    21781 tcacgatgat tcctcctcag atgattcagc gcttttattt tatcacttac gttattaaat
    21841 aaccatcgtt ttatataggg aattttctga aaattgttgc atatttttcg aaattttgtt
    21901 acttttaaaa aa
//
LOCUS       DACDKM010000037        21675 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20834_2016
            SAMN12640370-rid9891663.denovo.037, whole genome shotgun sequence.
ACCESSION   DACDKM010000037 DACDKM010000000
VERSION     DACDKM010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640370
            Sequence Read Archive: SRR10040891
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 21675)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21675)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742835.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742835.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:04:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,012
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 29 of 75
            Pseudo Genes (incomplete)         :: 33 of 75
            Pseudo Genes (internal stop)      :: 19 of 75
            Pseudo Genes (multiple problems)  :: 5 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21675
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640370-rid9891663.denovo.037"
                     /isolate="E20834_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(592..1983)
                     /locus_tag="IV334_001317"
     CDS             complement(592..1983)
                     /locus_tag="IV334_001317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-cystine transporter"
                     /protein_id="HAP8943397.1"
                     /translation="MTTLITVLVVLVFAALLYVFYRMQKRHTKFSTRVFTALGVGIVF
                     GGLLQLIFGVDDKVVTQSMEWIGIVGQGYVSFLQMLVMPLVFISIIGAFTKMKVSEKL
                     GKISATVLTSLLGTTAIAALIGIGSTMLFGLQGAKFTQGAAETARISELATRQETVEN
                     LSIPQQILSFIPKNVFADLAGSRATSTIGVVIFAAFVGIAYLGVKRKAPKEAAFFSDL
                     VDSLYKIIMRIVTLVLRLTPYGVFALMTNVLATSDFAAIVNLGKFVVASYVALLAVLI
                     VHSLILIGAKVNPINYFKKAFPVLSFAFTSRSSAGALPLNIETQTKALGVDDATANFS
                     ASFGLSIGQNGCAGVYPAMLATIVAPAAGVNVFSLEFILMLVAIVTISSFGVAGVGGG
                     ATFASLIVLGAMNLPVAIVGLVISVEPLIDMARTAVNVSGSMVAGIVTSARIKDLDRD
                     VINDDSKVIDVNA"
     gene            complement(2196..2582)
                     /locus_tag="IV334_001318"
     CDS             complement(2196..2582)
                     /locus_tag="IV334_001318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1149 family protein"
                     /protein_id="HAP8943398.1"
                     /translation="MEIRRQKEVVEAFHYDMRLPNQEVETDLKVGFSPLKETIENYPK
                     EHSIVGARLEFRLVFDTYVLSGSVSQINHILNRKIEGQNDVTQEEVDELVAPLFDIVR
                     RMSYEVTEIALDQPGVKLNFQSEKQE"
     gene            complement(2638..3261)
                     /locus_tag="IV334_001319"
     CDS             complement(2638..3261)
                     /locus_tag="IV334_001319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HAP8943399.1"
                     /translation="MKNYIWDFDGTLFDTYPAMVDGAWQALKDFGISMDKKEIYFKMK
                     KYSTSYLINESNLNAREFNELFHRYEKESTEVSRPFPETKQVLEMLKDNGGRHFILTH
                     RLTESTWGLLKEHRLAHLIEEVVGIDQDFPRKPDPASLNYLIDTFHLERTDTMMIGDR
                     RLDIEAGKNAGVATCLYDIDHFLGEIPADYVVGNLNEILTLQSIIKK"
     gene            complement(3325..3993)
                     /locus_tag="IV334_001320"
     CDS             complement(3325..3993)
                     /locus_tag="IV334_001320"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HAP8943400.1"
                     /translation="MKLVLLRHGESEANFENYWTGWLDVALTEKGKEQAAVAGQKMLE
                     AGLQFDAVYTSVLKRAILTGQVALEEMGQLWLPIIKTWRLNERHYGDLVGKNKDKMKE
                     IYGKDQVKTWRRGYTEVPPLTAENRFDRRYDHLDPHLIPYGESLEMTVNRIIPLWQDH
                     LAPKLKDRQDLLVVGHGNSIRALTKYLEDVPEDQMDTIDIPNAQPIQYTLADDLTILD
                     KKIL"
     gene            complement(4005..4445)
                     /locus_tag="IV334_001321"
     CDS             complement(4005..4445)
                     /locus_tag="IV334_001321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8943401.1"
                     /translation="MMQTWLERLIRFQQNYHKLMQGRYAKFDQLNRGLLIAALVFSLL
                     SRWLPYRIGQLLFIVFFGWMIFRFLSKKIYPRLNENQRYLKYLDRVKEKYHQIKNKKQ
                     VKMDRKSYTFFECPNCHQKQRAPKGKGRIRVTCKTCGIKFETNV"
     gene            complement(4504..5176)
                     /locus_tag="IV334_001322"
                     /pseudo
     CDS             complement(4504..5176)
                     /locus_tag="IV334_001322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327855.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
     gene            complement(5361..5759)
                     /locus_tag="IV334_001323"
     CDS             complement(5361..5759)
                     /locus_tag="IV334_001323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HAP8943402.1"
                     /translation="MKDLVALNLHMIVTIVDGGMGEDVIDFSKEAGAAGGTILHGRGS
                     GVHDSGKFLGLDISPEKDIVLTLVPDSLTNKVMDAIGQGINIDIPGNGICFSIDVDKV
                     IGITKIHQYREIIGMQELLDENNDREKSES"
     gene            complement(5756..6529)
                     /locus_tag="IV334_001324"
     CDS             complement(5756..6529)
                     /locus_tag="IV334_001324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1538 domain-containing protein"
                     /protein_id="HAP8943403.1"
                     /translation="MSFWQQVIDGIEIVTMEVILAVAPIVILFVLMNIISFRMRKKRF
                     FDIMKGFLITTIGLILFLHGVNMAYVPVGQHLGSAIAGLPYNYILVPLGFLMGFLVGF
                     AEPAIHVMIKQVEELSEGRIRSKIMLMVISIGIGAAVGLSMWRLLVGFSLYYFLIPGY
                     GLAFILGRKVDKIFLAMAFDNGGVATGPMCSTFILSMSVAIAAQIEGRDPIIDGFGVV
                     AMIALTPILSTLVLGYIYKRKDEKDRIEKERLQKLGESK"
     gene            complement(6526..7209)
                     /locus_tag="IV334_001325"
     CDS             complement(6526..7209)
                     /locus_tag="IV334_001325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1538 domain-containing protein"
                     /protein_id="HAP8943404.1"
                     /translation="MLKNFKEVIAAILPMTVLIVILTFVFAPLEGEDLASFLVGAAIM
                     MIGMTLFLFGADYSMMEVGDLVGKYMIKKKSLTVLVSLGFAIGIVITIAEPSVQVLGQ
                     QVYDISDGEIGRVLLIGIVSVGTGVFLAFALLRVVFKLSYYQLMAIGYIGVLIASFFT
                     SSEFMPIAFDSGGVTTGPVTVPFILALAGGMTSMIKQKKNENDSFGMVGIASLGPILA
                     VMILGVIFQ"
     gene            complement(7376..8263)
                     /locus_tag="IV334_001326"
     CDS             complement(7376..8263)
                     /locus_tag="IV334_001326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAP8943405.1"
                     /translation="MKNMVEKETIERPNVKKQALELRSTIGSVLKYTALSILSFISIF
                     PFVWMVLGMTNTPVDITSGKIKIGSNLIINFQNLFSNELNFTRSLWNSAMIALVTTVF
                     ALLISSMAGYGFEIYQSKRKDLIFNILLLSMMVPFAALMIPLYRMFSQLNGTVLGINS
                     FLVVILPSVSTAFLIFFFRQNVKSFPKDLVEAARLDGLKELSIFFRVYMPTAKNTYAA
                     AAIITFMSSWNNYLWPLVALQSPDKRTVPLVLSAMGASYTPDYGMIMAGLVIATLPTA
                     IVFFVLQKQFVQGMIGSVK"
     gene            complement(8260..9129)
                     /locus_tag="IV334_001327"
     CDS             complement(8260..9129)
                     /locus_tag="IV334_001327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP8943406.1"
                     /translation="MKQKDLFKNGHAFLILPVILISIMVFIPMVLALITSFQSGPPVN
                     MTFNGLGNYQRMLTDATFKKAFFNTFLYLLVQVPIMLFLALFISNLLNDKKLKYKGLF
                     RTAIFLPCITSMVSYSLIMKSLFSQSGLVNNLLLGIGMIDTPIGWLTHPIWARVLIIL
                     SITWRWTGYNMIFFLSGMQNIDSEIYEAASIDGASKWQQFIKVTIPNLKPIILFTSIT
                     STIGTLQLFDEVQNITGGGPANATTTLSQYIYNLSYKFTPNFGYAAAVSFVIVSFIVI
                     LSIIQTKVGGESR"
     gene            complement(9266..10552)
                     /locus_tag="IV334_001328"
     CDS             complement(9266..10552)
                     /locus_tag="IV334_001328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter substrate-binding
                     protein"
                     /protein_id="HAP8943407.1"
                     /translation="MNIRKLIAGVVCLAAASGFLTGCGSASSTADKEEKVTVWAWDET
                     FNIKAVNEAKKVYENEETEIEVVTMSQDDIVQKLNTALASGNTEGLPNIVLIEDYRIQ
                     GYLTSYPDAFANLSAIVKEDHFAPYKFAVNKVGDKIYGVPFDSGVTANFYRTDLMAEA
                     GYTEEDMENLKWDEYIQVARDVKEKTGKKIAEVNPSDLGRVRMIMQQAGEWYTSEDGE
                     TVTIQDNASLKYGLELFATLLKEDLVEQTSDWNAGVTAIQSGAVASSPTGAWYSSTIQ
                     GAENQSGKWKIAPIPALPENLQKAQASNLGGAGWYVIKGVAGEDSAKDFLEATFASNE
                     ELMGTLAKEIGLVSTMLSANEQEAYQEASEFYSGQKIFDDFSTWTSEIPEVNYGHETY
                     AIEAVVAEALHRIINGEETDKVLADTQKQVEAQLAN"
     gene            10825..11403
                     /locus_tag="IV334_001329"
     CDS             10825..11403
                     /locus_tag="IV334_001329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HAP8943408.1"
                     /translation="MPIRVILIDDEQTILDGMKQLINWSEHGFQLIGTFSSPLKALDF
                     SLVHAIDIVVTDVMMPELNGIELSRLLKQQQPETQILILSSYDEFDMVKDSFKEGVAD
                     YLLKPRLSPDFFLNSLTAVSQKIKKKPNKSPITNRYEQICENLSQQIAGQTRPLLDPL
                     LFEHTLFFCYIPKNGHSVQNKALNKNSRSPIH"
     gene            11403..12332
                     /locus_tag="IV334_001330"
     CDS             11403..12332
                     /locus_tag="IV334_001330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP8943409.1"
                     /translation="MDSDDSFTIYPVSTFSEESGYVINTDSKDTLRKFVQKFRQEVFP
                     GDFFVVSDSFAIQDINEIFQEIRNCTKGQRFYHKGKNLAYQHELLPLLTNHEQQASSY
                     LTKIVHKDYALAVKELENFFTTILEQPIDPAYLKHEAINRFYALLNALADEHVGNEEL
                     SLLKIALPSLLADAEYFEDFSILVKETLEKLTHFSANAKKDNFDILTLIYEFVQENYE
                     KEINLQLLSEKYHFSYSYLSAIFTEKFGINFSKYLKKVRISKAKLFLTETRLTLTEIC
                     EKVGYTELGYFSRVFKEETGMTPSQYRKGTFVK"
     gene            12329..14065
                     /locus_tag="IV334_001331"
     CDS             12329..14065
                     /locus_tag="IV334_001331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HAP8943410.1"
                     /translation="MRKKIKEHELFAKLLFILFIGLFLQAIVISLFIYHRSRDAYIQL
                     FNQSNDVVLKKIQSEFESLNDTIENTLAAFDSNPAVKSYFSNDPAQHMEQLQQLRTIQ
                     KMNDSLSKIHPMIDYDVLIFGENGRTFVGNDMLTAVSADSFFQSAIAQRVNERAADTQ
                     MLFAHHGLTLRDKKAPSVFFVKKLKNTLNHVYGYAVLSISSKQLANLFQSVVNPEISK
                     ISFMNEEQTIIASNEENTIGSRSMIFKKLTVGETTISNGQQLTRLPLYRQDCTLLSQV
                     DLHSLSNQMGVIFPIILYNIVTLAFAGSLVFMYLDRHTKSIYRLIDSLKRLEEDGLKA
                     TVPVQGSHEVQLLGNTINQLLSNAQHHYEQTIQDEKKKRSLEIQTMQAQIQPHFIYNT
                     LTSLKFLIWQKKTDEAVQGIDSFITLLRSTIGKKEEVIPVKEEIKSVQSYIDLLSMRY
                     GERISAKIMIPDELQSLSMPNMILQPIIENAYLHAFHNKTQGFITVYGKTKNQLLLFE
                     VIDNGDGFDTENTKQKNDHFSGIGISHVDERIRLMYGDAYGLTIQSVIGTGTTVTICL
                     PLLPKNQSEYQK"
     gene            complement(14128..14964)
                     /gene="pheA"
                     /locus_tag="IV334_001332"
     CDS             complement(14128..14964)
                     /gene="pheA"
                     /locus_tag="IV334_001332"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HAP8943411.1"
                     /translation="MNVSYLGPESSFTYQAACQLFPTEQLTAYASIPVCLRALFRKQV
                     DLAVVPVENSLEGAVHHTIDLLSKHPEVEVKSEIVLPIKQQLLGNPATKITKILSHPQ
                     ALAQSQQFLETHYPNVPLVATESTTAAAMYVAEHPKEDAAAIASLETAQHVGLEILAE
                     NIQDNELNQTRFWIVGDRKMTSQQSAPVKMSVILTLPANRPGMLHKMLAAFGWREINL
                     SKIESRPLKTSLGEYFFVIDLLLDRPMTLVENALQEIKMLGGESQILGCYPVLTVEET
                     RR"
     gene            complement(14961..15470)
                     /locus_tag="IV334_001333"
     CDS             complement(14961..15470)
                     /locus_tag="IV334_001333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HAP8943412.1"
                     /translation="MASILLIGFMGAGKTTIGKGLAQCLQKEYVDLDTKIEEHIQLSI
                     AEYFHYYGEKSFRKVESDILRKLSNEDKIIATGGGIVQSAENRRFLKTQPIVLYLEAE
                     ADCLVDRIQQDETSIRPLALGKTREEIKALLAQRLSWYEESATHRINTTNQTPEKIID
                     TIIERIKSI"
     gene            complement(15463..16758)
                     /gene="aroA"
                     /locus_tag="IV334_001334"
     CDS             complement(15463..16758)
                     /gene="aroA"
                     /locus_tag="IV334_001334"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HAP8943413.1"
                     /translation="MQLLQQIHGLRGTVRIPADKSISHRSIMFGAIAEGTTTIQNFLR
                     AEDCLSTLHAFQQLGVEIEEEEEVIKIHGRGSHSFVQPTESIDMGNSGTTIRLLMGIL
                     AGQPFTTTLFGDASLSKRPMGRVMEPLREMGADLQGEENDQYLPITVTGTRSLSPIRY
                     NMPVASAQVKSALLFAALQAEGTSVIVEKERSRNHTEEMIRQFGGRITVEDKTIIVTG
                     PQKLTGQQITVPGDISSAAFFLAAGLLVPESQLLLKNVGVNPTRTGILDVLEEMGAAI
                     TQTNHNEQNQSADLSVKTSHLKKATIGGEIIPRLIDELPILALVATQAEGITIIKDAE
                     ELKVKETNRIDAVAEELQKMGAKIKATADGLIIHGPTPLHGAKVSSRGDHRIGMMLQV
                     AALIADGPCELEGAEAVSISYPAFFDDLAELVSGGEAHG"
     gene            complement(16771..17862)
                     /locus_tag="IV334_001335"
     CDS             complement(16771..17862)
                     /locus_tag="IV334_001335"
                     /EC_number="1.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase"
                     /protein_id="HAP8943414.1"
                     /translation="MAQHVFIVGLGLIGASMALCIRQTNPELIVVGWDSQSKTREQAV
                     EQQIVDYVASDFETGAEQADVILLAVPVRTTLAYLDVLERINLSDHVIITDVSSTKQQ
                     VVTSAEQKNLRFVGGHPMAGSHKSGIQAADAKLFENAYYIFTPLEKTKKDVEKLQELF
                     CGTKAKYVTLTAREHDRITGMLSHFPHILAAGLVNQAEQFNQEYPRAKQLAAGGFRDI
                     TRIASSDPVMWTDILLSNKQILLERLADWQQEMTQIAEWIMTENQSEIFSFFNRAKES
                     RDQLPVHKQGAIPAFFDLFIDVPDEPGVIAEVTGLIGKAGVSLINLKIQETREDILGI
                     LQISFKNQQDLLQAKRIIMSNTSYHCWIK"
     gene            complement(17879..19045)
                     /gene="aroC"
                     /locus_tag="IV334_001336"
     CDS             complement(17879..19045)
                     /gene="aroC"
                     /locus_tag="IV334_001336"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HAP8943415.1"
                     /translation="MRYMTAGESHGPELTAIIEGLPAGLPLSEEEINHELARRQAGYG
                     RGGRMKIEKDRVRITSGIRHGKTIGSPVTLVVENKDWKNWTTVMSVAEVPEKDQSLRR
                     VARPRPGHADLVGGMKYHHRDLRNVLERSSARETTMRVAIGAIAKKILEELDIIVAGH
                     VICLGGIQAEIPGNISIQEIKEISEASEVRVVDPAVEQRIKDLIDQTKKNGDTIGGIV
                     EVLVGGVPAGLGSYVQWDTKLDAKIAQAVVSINAFKGVEFGVGFTSGSLPGSEVMDEI
                     LWEEERGYFRRTNNLGGFEGGMTNGEPLVIRGVMKPIPTLYKPLQSVDIDTKEPYKAS
                     VERSDSTAVPAASVVAENVVATVVATELLSKFPSDSLVELKAAVKEYRDYLKHY"
     gene            complement(19047..20111)
                     /gene="aroB"
                     /locus_tag="IV334_001337"
     CDS             complement(19047..20111)
                     /gene="aroB"
                     /locus_tag="IV334_001337"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HAP8943416.1"
                     /translation="MLTVHVDNQKYDIHIEKGALEQAGSWLQTIWRPQKVAVITDTNV
                     APLYAEKIMFQLRQANFEPVLSIVPAGETSKSLEQAAELYDFLADNDFTRSDGILALG
                     GGMVGDLAAFVASTFMRGIHFVQIPTTLLAQVDSSIGGKTAVNTNKAKNLVGTFSQPA
                     GVLIDPEVLKTLPVRRLREGIAEIIKSAAIADATLWKQLNELKDEKDLLEHAVPIITA
                     TLEIKRKVVEEDVFDQGNRLILNFGHTIGHAIENTAGYGVVSHGEGVAIGMVAITSHA
                     EKIGLSPTGSTAALERILQKFHLPITYDLSHPDELFHAITHDKKARGSQLNIILLEKI
                     GQAKIVPVPTETFKDYLGGL"
     gene            complement(20144..21166)
                     /gene="aroF"
                     /locus_tag="IV334_001338"
     CDS             complement(20144..21166)
                     /gene="aroF"
                     /locus_tag="IV334_001338"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HAP8943417.1"
                     /translation="MIVIMKKEATEEQIKKVVDHIEKEGFKVSIDQGTERVVIGLKGD
                     TRSLQEGAFTRYEGVENAVRILNTYKLTSREFHPGNTVVDVDGVKIGDGSFVTMAGPC
                     SIEGLDQIRECARMAKAGGAKILRGGAFKPRTSPYAFQGLEEEGLKYIRQAADEFGMK
                     VITEVMDEGHIDMVAEYSDILQIGARNMQNFKLLSAVGKTGKPVGLKRGISGTINEWL
                     NAAEYIAVEDKSPVIFIERGIRTYETATRNTFDLSAVPLMKKLTHFPVIVDPSHGTGI
                     WELVPPMARAGVASGADGMIVEIHPDPANAWSDGPQSLNEKTYSRMMKEVAIMKEAME
                     KIQTLD"
BASE COUNT         6280 a         4727 c         3781 g         6887 t
ORIGIN      
        1 ctgttctaac attctcaact ttgtcagttt accataggaa aatgggaaaa tctacgaaag
       61 ctctcaacag aatcgtccta cttaaatacg cttttcttta ataataaata agttggttgt
      121 ttttgttatt tacgctttca cttttcggaa taaataatgt gtatttatat ggaataaata
      181 aaaaaagagg tcgtgactaa aaacaagtca caacctctgg taaacggtgt tctcctgaca
      241 actcctcggt cacaagacac aatattcaac aaacatgaga agctattttt tgaaattgct
      301 ctttgtgact cagagtgaaa cggcaggctc acgacctctt tcttaaacgg tgttcaaaag
      361 cttgcatctg cggtaataag ctcaaccaaa cgtaaaatat gaggaactat ttttgtttgg
      421 ctgttcttat tacttgatgc agggcagctt tttcacaacc tcttattcac ggtgtttcag
      481 aagtagcttc tcggaaataa gccgaaattt cacaaaaatt tgagaaacta tttttaggaa
      541 acttcttctt atttcttaaa gctgaccact tctatcacaa cctctttttg actatgcatt
      601 cacatcgatc actttagaat catcattgat gacatcgcga tctaaatctt tgatgcgtgc
      661 acttgtcacg ataccagcta ccatgctacc gctgacgttt actgctgtac gtgccatatc
      721 gattaaaggt tcaacagaga taactaaccc aacgattgct actggcaagt tcattgcccc
      781 taaaacgatc agtgaagcaa atgttgcgcc cccaccgact ccagctacac caaaagaact
      841 gattgttaca attgccacaa gcatcaaaat aaattccaaa ctaaagacat tcactccagc
      901 tgctggtgcg acgatcgttg ccaacatagc tggataaacg ccagcacagc cgttttgtcc
      961 aatagacaaa ccaaaacttg ctgagaaatt agctgttgca tcatctactc ctaaagcttt
     1021 tgtctgagtc tcgatattca atggcaaagc tccagcactt gaacgtgaag taaacgcaaa
     1081 gctcaatacc ggaaaggctt ttttgaaata gttgattgga ttgacttttg caccaatcaa
     1141 aatcagtgag tggacaatca aaacagcaag caatgctacg taagaagcaa cgacaaattt
     1201 acctaaattg acgatagctg caaaatcact tgttgccaaa acgtttgtca tcaaagcaaa
     1261 tactccataa ggtgtcaaac gcaaaacaag cgtcacgatc cgcataatga ttttatacag
     1321 actgtcgact aaatctgaaa agaatgctgc ttcttttggc gcttttcttt tcacgcctag
     1381 ataagcgatt ccgacaaatg ccgcgaaaat aacgactcca atcgtacttg ttgcacgact
     1441 acctgctaaa tcagcaaaaa cgtttttagg gataaaggat aaaatctgct gtggaatcga
     1501 taagttttca actgtttcct gacgtgttgc cagttcagag attcgtgctg tttctgcagc
     1561 tccttgtgtg aatttagctc cttggagtcc gaacagcatt gttgaaccaa ttccgatcaa
     1621 tgctgcaata gctgttgttc caagtaaaga agtcaataca gtcgcactaa ttttccctag
     1681 cttttcagaa actttcattt ttgtgaatgc accaatgatc gaaataaaca ctaaaggcat
     1741 aactagcatt tgcaagaatg atacatatcc ttgacctaca atcccgatcc attccattga
     1801 ttgagtaacg actttgtcgt ctacaccaaa gatcaattga agcagaccgc caaagacgat
     1861 accaactcct aatgctgtaa agacgcgtgt tgaaaatttt gtatggcgct tttgcatacg
     1921 atagaacaca tataataatg ccgcaaaaac tagaaccact aaaactgtga tgagtgttgt
     1981 catttttctc ctcctcatat tattaatgac aagtccatcg aaggaagtaa aggttcccga
     2041 gaatgattct tcaaccatcc tcctgaaaaa aattttttca gtgactttta tattttagac
     2101 gatttttatc gaaatgacat tctttttgct tacaattagt aataaaaaaa catacctgtg
     2161 atagctttaa tctatcgcag gtatgttttt actgtctatt cttgtttttc tgattgaaaa
     2221 ttcaatttta ccccaggctg atctaaggcg atctctgtca cctcataaga cattcggcgt
     2281 acgatatcga ataacggtgc tactagctcg tccacttctt cttgtgtgac atcattttga
     2341 ccttcaattt tccgattgag gatatgattg atctgactca cacttccaga taaaacatat
     2401 gtatcaaaca ctaaacggaa ctctaagcgt gctcctacga tgctatgttc tttcggatag
     2461 ttttcgatcg tttcttttaa aggcgagaag cctactttca aatctgtttc tacttcttgg
     2521 ttcggcaatc tcatatcata atgaaatgct tctacaactt ctttttgtct tctgatttcc
     2581 atataatttt atcactcctt tatgaaaatt ataacatatc tcctttgaaa ggggatacta
     2641 ctttttaatg atgctctgca aggtaaggat ttcattcaga tttcctacca cataatctgc
     2701 cgggatctca cccagaaaat gatcgatgtc gtaaagacaa gtagcgactc ctgcattttt
     2761 ccctgcttcg atatctaaac gacgatcgcc gatcatcatc gtgtctgttc tttctaaatg
     2821 aaaagtatcg atcaaataat ttaaagatgc tggatcaggt ttacgaggaa agtcctgatc
     2881 gatccctact acttcctcaa tcagatgtgc caatcgatgt tcttttaaca ggccccatgt
     2941 tgattctgtc aaacggtggg tcaggatgaa atgacgaccg ccgttgtctt ttagcatttc
     3001 cagtacttgt ttcgtttctg gaaatggacg ggaaacttcc gtactttctt tttcatatcg
     3061 gtgaaataat tcattaaact ctctggcatt caagtttgat tcattgatca aataactagt
     3121 agaatacttt ttcattttga agtagatttc ttttttgtcc atactgatac caaaatcttt
     3181 tagtgcttgc catgcaccat ctaccattgc gggatacgta tcaaacaatg taccatcaaa
     3241 gtcccatata taatttttca tgttcctttt gttccattcc tttgcttctt ttttctatct
     3301 ctcactttta gataaaggtg ttctttaaag tatttttttg tccagaattg tcaaatcatc
     3361 tgctaatgta tactggattg gttgtgcatt tgggatatcg atcgtatcca tttgatcttc
     3421 aggaacatct tctaaatatt ttgtcagtgc tcggatgcta ttaccatgac ccacaactag
     3481 taaatcttgc cgatctttta gttttggtgc tagatgatct tgccataagg gaatgatccg
     3541 attcactgtc atttccagac tttctccgta tgggatcaaa tgcggatcca agtgatcata
     3601 tctgcggtca aatctatttt ctgcggttaa cggcggaact tccgtatatc ctctgcgcca
     3661 tgtttttact tgatcttttc cgtatatttc tttcatctta tctttgtttt tacccacaag
     3721 atccccataa tgccgttcat ttagacgcca tgtttttatt ataggaagcc ataactgacc
     3781 catttcttct agtgcgactt gtccggtaag aatcgcacgt ttcaaaacag aggtataaac
     3841 ggcatcgaat tgaagtccgg cttcaagcat tttttgaccg gcaactgctg cttgttcttt
     3901 tcctttttct gttaaggcga catctagcca tcctgtccag taattttcaa aatttgcttc
     3961 gctttcgcca tgacgtaata atactaattt cattcttttc tcccttaaac attcgtttcg
     4021 aattttatac cacatgtttt gcatgtcaca cgtattcgtc cttttccttt aggtgcacgc
     4081 tgcttttgat gacaattcgg gcactcaaaa aaagtataac tttttcgatc cattttgact
     4141 tgctttttgt tttttatctg atgatatttt tctttgactc tatcaagata tttcaaataa
     4201 cgttgattct catttagtct ggggtagatt ttttttgata ggaaacgaaa tatcatccat
     4261 ccgaaaaaga cgatgaacaa caattgtccg attctatagg gcaaccaacg acttagcaaa
     4321 ctaaagacaa gcgcagcgat caatagaccg cgattcaact gatcaaattt cgcatatctt
     4381 ccctgcataa gtttgtgata gttctgctga aatcgtatta gcctttctaa ccatgtttgc
     4441 atcatattat ctcctccatc atctaatatt tattatgaca aaaaaatcca aaaagaaaaa
     4501 gccttaattg gccttttcca ttgcaacttg gtgaaggaca ttccgatttc tggtaaagtc
     4561 aatcacaatc actttctttt tatcttctct gttgactact cgaaatgtgt ccgtcaatgg
     4621 aagaaacagc ttcttagatg gtagctgttg catacgtata aaacgatgga tcatcgtaat
     4681 caattgagga aattgaatag acgtgtgaag cagacctgga tattttttca acagctgtgt
     4741 cgttttgatc agattccgtt tttttactga tcacttttaa aaagcttttt agaatctttt
     4801 gctgacgtct aaaaatagaa aaagaatttt cgtcatctat ttgataacgc atttgttttt
     4861 gcagttctgc tgcagtcaga tggatcttcc catcgtttgt atcgatcatt aaaccttctg
     4921 gaaacagttc aaccactgct cgatcccaag attcaagaga aagctcgata taatctttta
     4981 ctggtaattc caattctacc ttaaaaaagt gcaaaacagc cgacagccct tctgcttgat
     5041 agatttctcc taatgttagc ccctctagat ggaactctgg tgaaaaatgc agcaatgttt
     5101 tattttcctg caattgaaca tatagaaatg tagatcgctc aatcagagta tcttttaaaa
     5161 caactaaaaa attcattttt ctcccccttt tttcatcatt tttaaaatag catattttga
     5221 aaagaaagtc tgatgcttag agtagaattt cataaaaatt tactatttca taagtattca
     5281 cgagaaaaat acaaacaaaa aagcggaaat aacgctcatc cgccttcttg tttgtgttta
     5341 tttcctcttc aaacgcttgt ctatgattca gacttttctc tatcgttatt ttcatctaaa
     5401 agttcttgca ttccgataat ttctcgatat tgatgaatct tagtgatacc aatgactttg
     5461 tctacatcaa tactaaaaca aataccatta cctggaatat cgatgttgat gccttgacca
     5521 atcgcatcca ttactttatt cgtcaaacta tcgggtacta atgtcaacac aatatctttt
     5581 tctggcgaaa tgtccaaacc taaaaatttc cctgaatcat gtacaccaga acctcttcca
     5641 tgtaagatcg ttccgcctgc tgcacctgct tcttttgaaa agtcgataac atcttcgccc
     5701 attcctccgt caacgatcgt cacaatcatg tgaagattca atgctacaag atctttcatt
     5761 tgctttcccc caatttttgc aatctttctt tttcgattct gtctttttca tcttttcgtt
     5821 tataaatata tcctagtact aaagtggata atattggtgt caatgcaatc attgcaacca
     5881 caccaaaccc gtcaatgatc ggatctcttc cttcaatttg tgcagcgatt gccacgctca
     5941 ttgataaaat aaatgtcgaa cacatcgggc ccgttgctac tccaccgtta tcaaaagcca
     6001 ttgctaaaaa gatcttgtcc acttttcgtc ctaaaataaa tgctagcccg tatcctggga
     6061 tcaagaaata ataaagagag aatccgacga gcaaacgcca catagacaat cctactgctg
     6121 cgccgatccc aattgaaatt accatcagca tgattttcga tcggattctc ccctcgctca
     6181 attcttcaac ctgtttgatc attacatgaa tcgccggctc agcaaaccct actaagaacc
     6241 ccattaaaaa gccaagggga accagtatat aattgtaagg caatcccgca atagcactgc
     6301 ctaaatgctg tcctaccggt acataagcca tattgactcc gtgcaggaaa aggatcaacc
     6361 caatagtcgt tatcaagaaa cctttcataa tgtcaaagaa acgctttttg cgcatacgaa
     6421 aactaatgat attcattaaa acaaatagga ttacgatagg tgccactgcc aaaatgactt
     6481 ccatggtaac gatttcgatt ccatctatca cttgctgcca gaaactcatt ggaagatcac
     6541 ccccaaaatc atcacggcaa gaatcggccc taacgaagca ataccgacca ttccaaaact
     6601 gtcattttca tttttctttt gtttgatcat actagtcatc cctccggcaa gggccaagat
     6661 aaatggaaca gttactggac cagtagtcac tccccccgaa tcaaaagcaa tcggcataaa
     6721 ttcggaactg gtgaaaaatg aggcgatcaa aacgcctatg tagcctatag ccatcaattg
     6781 ataataggat agcttgaata cgacccgaag cagcgcaaag gctagaaata ctccagtgcc
     6841 gacgctgacg atcccaatca gcaacactct gccaatctct ccgtcagata tatcatatac
     6901 ttgttgtccc aaaacttgca cggaaggttc agcaatcgtg atcacgatcc cgatagcaaa
     6961 tcccaaactg accaaaacgg tcaaactttt ctttttgatc atatatttcc ctactagatc
     7021 tccgacttcc atcatagaat aatctgctcc aaacaaaaag agcgtcattc cgatcatcat
     7081 gattgccgca ccaaccagaa atgaagcgag gtcttctcct tctaacggtg caaaaacaaa
     7141 cgttaaaatg acaatcagga cagtcatcgg caatatagca gcaatcactt ctttaaaatt
     7201 ttttagcatg tatcctcctc ctttactctt taagtttacc ttaaaatgaa agagttttcg
     7261 agcacaaact taaaacgtta tcatttaatt tttctcaaca taaaataaca cacgtcccaa
     7321 aagtatcttt ttacttggaa aaatgacttt gaaggaacgc gcttattgtg tttttttatt
     7381 ttacagagcc gatcattcct tgaacgaatt gcttttgcaa aacaaaaaat acgatggcag
     7441 ttggtaaagt agcgatgact aatccagcca tgatcattcc atagtctggt gtataggaag
     7501 cacccatagc agataaaaca agcggaaccg tccgtttatc aggagattgc aaagctacta
     7561 agggccataa ataattgttc cagctactca tgaatgtgat gattgcagcc gcggcatagg
     7621 tatttttagc cgtcggcata tacactctaa aaaaaatact tagttctttt agaccgtcta
     7681 atcgagcggc ttctactaga tcttttggaa aagatttaac attttgtcga aagaagaaga
     7741 tcaaaaaagc agtgctaaca gatggcagga tgacaaccaa gaaactgttg attcccaaaa
     7801 ccgttccgtt caattgtgaa aacattcgat ataaagggat catcaatgcc gcaaaaggaa
     7861 ccatcatcga tagcagtaga atattgaaaa ttaggtcttt tcgtttactt tgataaatct
     7921 caaatccata tcctgccatt gacgaaatca gcaatgcaaa cacagtggtc accaaagcga
     7981 tcatcgctga attccaaagg gaacgagtaa aattcagctc attggaaaac aaattttgaa
     8041 aattgatgat caagttactg ccaatcttta tttttcccga tgtgatatcc acgggtgtat
     8101 tcgtcattcc caaaaccatc cagacaaaag ggaaaatgga aataaatgat aggatgctta
     8161 atgctgtata ctttaatact gacccaattg tcgaacgaag ttctaatgct tgttttttga
     8221 cgttggggcg ttcgattgtt tctttttcta ccatattttt catcgacttt ctcctcctac
     8281 tttcgtttgg attatcgaca atatcacaat aaaggaaaca ataacaaagg aaactgctgc
     8341 cgcatatcca aaattaggcg tgaatttata actcaaatta tagatgtatt gagatagggt
     8401 ggtcgttgca tttgctggtc ccccgccagt aatattttgt acttcatcaa aaagctgcaa
     8461 tgtcccgatc gtcgaagtaa tggaagtaaa caaaatgatt ggttttaaat ttgggatcgt
     8521 cactttgata aactgctgcc atttagaagc accgtcaata gatgctgctt cataaatctc
     8581 cgaatcaata ttttgcatgc ctgaaaggaa aaaaatcata ttgtatccag tccaacgcca
     8641 agtgatcgaa agaatgatca gaactctcgc ccaaattgga tgagtcaacc agccaatcgg
     8701 tgtgtcaatc ataccaatgc ccaacaacaa gttgtttaca aggccagatt gagaaaatag
     8761 acttttcatg atcagagaat aactgaccat cgaagtgata catggaagaa agatagcggt
     8821 tcgaaacaac cctttatatt tcaacttttt atcatttaaa aggttggaaa taaaaagtgc
     8881 tagaaacaac atgatcggta cttgtacaag taaatacagg aatgtattaa aaaaagcttt
     8941 tttaaaagtt gcatctgtca acatccgctg ataattacct aacccgttaa atgtcatatt
     9001 gacaggggga ccactttgaa aagacgtgat caaagccaaa accatcggaa taaaaaccat
     9061 gattgatatc aaaatgactg gcaagattaa aaaagcatgc ccgtttttga ataaatcttt
     9121 ctgcttcatt cttttccctc caattctatc attgattatc tacatgaaaa gacaagaaag
     9181 acaatatctg taaggcagac gtgatctttt ttgtcttttc gtttgtctga tgaaaaagcg
     9241 aagaaattca cttttttacc accgatcaat tagccaattg tgcttctact tgtttttgtg
     9301 tatctgctag taccttgtct gtctcttctc cattgatgat tcgatgaaga gcttcagcta
     9361 cgactgcttc aattgcatag gtctcatgtc cgtaattcac ttctggtatt tcagatgtcc
     9421 atgttgaaaa atcgtcaaag atcttttgac cactgtaaaa ttcagaagct tcttgataag
     9481 cttcttgttc attcgcgcta agcatcgtag atactagtcc gatttctttc gcaagagtgc
     9541 ccatcagttc ttcattcgaa gcaaacgttg cttctaggaa atctttcgct gaatcttctc
     9601 cagctactcc tttgatcaca taccagccag cgccgccaag gttagaagct tgcgcttttt
     9661 gcagattttc tggaagtgca ggtattggtg caatcttcca ttttccagat tggttttctg
     9721 ctccttggat cgttgaagaa taccaagcgc cagttgggct agaagctact gctcctgatt
     9781 gtatcgcagt tactccagca ttccagtcag aggtttgttc gactagatct tctttcaaaa
     9841 gagtggcaaa caattcaaga ccatacttta aagatgcatt gtcttggatc gtgactgtct
     9901 ccccatcttc agatgtatac cattctcctg cctgttgcat aatcatcctt actcgaccaa
     9961 ggtcagaagg attgacttca gcgattttct ttcccgtctt ttctttgaca tctcgcgcta
    10021 cttgaatata ctcgtcccac ttcagattct ccatatcctc ttctgtataa cctgcttctg
    10081 ccatcagatc cgtacgatag aagtttgctg ttacgcccga atcgaaagga acaccgtata
    10141 ttttatctcc tactttgttt accgcaaatt tatacggtgc aaaatgatct tcctttacaa
    10201 tagcagacaa attagcgaat gcatctggat aagaagtcaa atagccttgt atcctataat
    10261 cttcaatcaa gacgatattg ggcaatcctt ctgtattccc gctcgctaaa gcggtgttca
    10321 gtttctgaac gatatcatcc tgagacatcg taacaacttc tatctctgtt tcttcgttct
    10381 cataaacttt ttttgcttca ttcactgctt tgatattgaa tgtctcatcc cacgcccaga
    10441 cagtgacttt ttcttctttg tctgctgtac tactagcact tccgcatcct gttaagaaac
    10501 ctgatgctgc cgctaaacaa acgactccag caattaactt tctgatgttc atttttatcc
    10561 gctccttttc catccattag ttgtttacgt ttgctacatt catcatacca aaatttgaaa
    10621 acggtttctt tattattcag atacttcaaa aatttctcct aaaataataa aaaaatacaa
    10681 atcaactatg acaaaaggaa taatcttagc atctagacca aagatcgaaa tggtgaggtt
    10741 tctttaaaat aaaaatatcc ttgcattttc tcctagaaat aagtattttt aaattacaag
    10801 actattctta aggcgggtaa aaaaatgcca atacgcgtaa ttcttatcga tgatgaacag
    10861 acgattttag atggaatgaa acagttgatc aattggtctg agcacgggtt tcaattaata
    10921 gggacttttt ccagtccgct gaaagcgctt gatttttctt tggtacatgc aatcgatatc
    10981 gtggtcacag atgtaatgat gcctgaatta aatggaatcg aattatcgcg gctattaaaa
    11041 cagcaacaac ctgaaacaca gatccttatc ctctctagct atgatgaatt tgatatggtt
    11101 aaagattctt ttaaagaagg cgtcgctgat tatcttttaa agcctcgatt gagtcctgat
    11161 ttttttctaa attccttaac tgccgtttca caaaaaatca aaaaaaagcc caacaaaagc
    11221 ccaatcacca atcgttatga gcagatctgc gagaatcttt cacagcagat tgctggacaa
    11281 actcgtccat tgcttgaccc tcttcttttt gaacacacct tgtttttctg ttatataccg
    11341 aaaaacggcc attcagttca aaacaaggct ctaaacaaaa acagtcgatc acccattcat
    11401 tgatggatag cgacgattcg tttactattt atcctgtttc taccttttcg gaagaatccg
    11461 gctatgtaat caatactgat tccaaagata cacttaggaa gtttgttcaa aagtttcgtc
    11521 aagaagtttt tccaggtgat ttttttgttg tttctgattc ttttgcaatc caagatatca
    11581 atgaaatatt ccaagagatc agaaattgca ccaaaggaca acgtttctat cataagggaa
    11641 agaaccttgc ttatcagcat gaacttcttc cgttattaac caaccacgag cagcaagcaa
    11701 gtagttattt aacaaaaatc gttcataaag actacgcttt agctgtcaaa gaacttgaga
    11761 atttcttcac gacgatattg gaacagccta ttgatccagc ttatttgaaa catgaggcaa
    11821 tcaatcgttt ctatgcatta ctcaacgctt tggctgacga gcatgttggg aatgaagaac
    11881 tttctttgct caagatcgcc cttccttctt tattagccga tgctgagtat ttcgaagatt
    11941 tttctattct tgtcaaagaa acacttgaga aactcaccca cttttctgca aatgcaaaaa
    12001 aagacaattt cgatatcttg actttgatct acgagttcgt ccaggaaaat tatgagaaag
    12061 aaatcaattt acaactttta tccgaaaaat atcatttttc atatagctat ctctcagcca
    12121 tctttactga aaaatttggt atcaactttt caaaatatct aaaaaaagtc cgtatcagta
    12181 aagccaagct ctttttgacg gaaacacggc ttactttgac ggaaatctgt gagaaagtcg
    12241 gctataccga actgggttat ttctccagag tttttaaaga agagacagga atgacacctt
    12301 ctcaataccg taaaggaact tttgtaaaat gagaaaaaaa atcaaagagc atgaactctt
    12361 tgccaaactt ctgttcattt tgttcatcgg tctttttctg caagccatcg tcatttcttt
    12421 gtttatttat catcgatcaa gggatgccta tatccagctg ttcaatcaat cgaatgatgt
    12481 ggtactaaaa aagattcaat ctgaatttga aagtttgaat gatacaatcg agaatacatt
    12541 agccgctttt gattctaatc cagctgtcaa aagctatttt tccaatgatc cagcccaaca
    12601 tatggaacag cttcagcaac taagaacaat ccaaaaaatg aatgactctc tttcaaaaat
    12661 ccatccaatg atcgattacg atgtattgat ctttggagaa aatggaagaa cttttgtcgg
    12721 aaacgacatg ctcactgccg tatctgccga ttcttttttt caatctgcga ttgctcagag
    12781 agtgaatgaa cgtgctgctg atacacaaat gctttttgca caccacggat tgactcttcg
    12841 cgataagaaa gcacccagcg tcttctttgt caaaaaactg aaaaatacat tgaatcacgt
    12901 ttacggatat gctgttttat cgatctcatc caagcaacta gcgaatcttt ttcaatctgt
    12961 agtaaatcct gagatatcga agatctcctt catgaacgaa gaacagacga tcatcgcatc
    13021 aaatgaagaa aatacaattg ggagccgatc aatgattttc aaaaaactaa cagtaggtga
    13081 aacaacgatt tcaaatggac agcagttgac ccgtcttcct ctgtaccgcc aggattgtac
    13141 attactcagt caagtcgatc ttcattcttt atctaaccaa atgggcgtga tttttccgat
    13201 tattttgtat aatatcgtga cacttgcttt tgctggctca ttggtattta tgtatttgga
    13261 tcgacacaca aaatcgatct accgcttgat tgattcgcta aaacggctag aggaagatgg
    13321 cttaaaagct actgttcctg tacaaggaag ccatgaagtc caattgctag ggaatacgat
    13381 caatcaattg ctaagtaatg ctcagcatca ttatgaacag acgatccaag acgaaaagaa
    13441 gaaacgttcg cttgaaatcc aaactatgca agcacagata cagccccatt ttatttataa
    13501 tacgctgact agcttgaaat ttttgatttg gcagaaaaaa acagatgaag cagttcaagg
    13561 aatcgacagt ttcattactt tactaagaag tacgattggg aaaaaagaag aagtgattcc
    13621 agtcaaggaa gaaatcaaaa gcgtacaaag ttacatcgat cttttgagta tgcgatatgg
    13681 cgaaagaatc tcggctaaaa taatgattcc tgatgaactg cagtcattat ctatgccgaa
    13741 tatgatcctt caaccaatta tcgaaaacgc ctatcttcat gcttttcaca ataaaacgca
    13801 aggattcatc acggtgtacg gcaaaacaaa aaatcagctt cttttattcg aagtgattga
    13861 taacggcgat ggcttcgata ctgaaaatac caaacaaaaa aatgatcatt tttcaggaat
    13921 cggcatcagc catgtagatg agcggatacg cctaatgtat ggcgatgcat atggcttaac
    13981 gatccaatca gtgattggga caggaacgac tgtaacgatt tgtctaccgc ttcttcctaa
    14041 aaatcaatca gaatatcaaa aataaagaat agccaagcgc tgattttttt attttaagtt
    14101 aaataaatca atcagtattc ttgactttca tctacgcgtc tcttctactg tcagcactgg
    14161 ataacagccc aatatttgac tttctcctcc gagcatcttg atttcttgga gcgcattctc
    14221 tactaatgtc attggacggt ccaacaacaa atcgatcacg aaaaaatatt ctcccaagct
    14281 agttttcaat ggtcgggatt caatcttact taagttgatt tcccgccagc caaaagctgc
    14341 cagcatttta tgaagcatcc ctggccgatt agcaggtagt gtcaagatga cactcatttt
    14401 tactggagct gactgctggc tggtcatctt tcggtcaccg acgatccaaa aacgtgtctg
    14461 gttcaactca ttatcttgga tattctcagc taagatctcc aatccaacat gctgtgctgt
    14521 ttctaatgaa gcaatcgctg ctgcgtcttc tttcggatgt tctgctacat acattgctgc
    14581 agcagttgtt gattctgttg ctactaaagg aacattagga taatgcgttt ctaaaaactg
    14641 ttgtgattgt gctagtgctt gtggatgtga aaggattttt gtaatctttg ttgctggatt
    14701 gcctaaaagc tgctgcttga taggaagaac gatttcactt ttaacttcga cttctggatg
    14761 tttggaaaga agatcgattg tgtgatgaac agctccttcc aatgaattct ctacaggaac
    14821 gacagccaga tcgacttgtt tgcgaaacaa agcgcgcaaa caaacaggga tggaagcata
    14881 cgcagtcaat tgttctgtag gaaatagctg acaagccgcc tgataagtga atgaactttc
    14941 tggccctaga tagctgacgt tcatatgctt ttgatccttt ctataatggt atcgatgatc
    15001 ttttctggtg tttgattggt cgtgttgatt cgatgtgtcg cactttcttc ataccaagaa
    15061 agtcgttgtg ccaataatgc ttttatttct tctcttgttt ttccaagagc taaaggtcga
    15121 atagacgttt catcttgttg gatacgatca actagacagt ccgcttctgc ctctaagtaa
    15181 agaacaatcg gctgtgtttt taaaaaacgc cgattttctg cactttggac aatccctcca
    15241 ccagtcgcga taattttgtc ttcatttgac agctttctta aaatatctga ctccactttt
    15301 cgaaagcttt tttcaccgta atagtgaaaa tattcggcaa tcgacaattg gatatgttcc
    15361 tcaatctttg tatctaaatc tacatactct ttttgtaaac attgagctaa ccctttaccg
    15421 attgttgttt ttccagcgcc catgaaacca atcagcaaaa tgctagccat gagcttctcc
    15481 tcctgacaca agttctgcta aatcgtcaaa aaatgccgga taagagattg aaacagcttc
    15541 agctccttct agttcgcaag gtccatcagc tatcaaagca gctacttgca gcatcatacc
    15601 gattcgatga tcgcctctgc tggacacttt tgcgccatgc agaggtgtcg gcccatggat
    15661 gatcagaccg tctgctgtcg cttttatctt tgctcccatt ttctgtaatt cttctgccac
    15721 cgcatcaatt ctgttggttt cttttacttt taattcttct gcgtctttga tgatcgtgat
    15781 tccttctgct tgagtagcca ctaaagctag aatgggcaat tcatcaatca gacgcggaat
    15841 gatttctcca ccaatcgttg ctttcttcaa atgactcgtc ttgacactta aatcagccga
    15901 ttggttctgt tcattgtgat tcgtctgggt aatcgctgcg cccatctcct ccaacacatc
    15961 taagatacca gttcttgttg gattgacccc gacatttttt aacaacagtt ggctttccgg
    16021 aacaagaagt cctgctgcta gaaagaaagc agctgatgaa atatcacctg gaacagttat
    16081 ctgttgacca gttaattttt gcggtccggt cactatgatc gttttatctt ccactgtgat
    16141 ccttccgcca aattgacgaa tcatttcttc cgtatggtta cgggaacgtt ctttttcaac
    16201 gattacggat gtgccttctg cttgtaatgc cgcaaacagc aaagcagatt tgacttgcgc
    16261 actggctaca ggcatattgt atcggatagg tgataaagag cgggttcctg tcacagtgat
    16321 tggtagatac tggtcatttt cttctccttg caagtcagcg cccatctcgc gtaaaggttc
    16381 catcactcgc cccattggac gtttagacaa cgaagcatcc ccgaacagag tagtcgtaaa
    16441 aggctgtcca gccaaaatac ccatcaataa acgtatggtc gtaccggagt ttcccatgtc
    16501 aattgattcg gttggttgga caaatgagtg gctaccgcga ccatgaatct tgatcacctc
    16561 ttcctcttct tcgatctcga cacctaattg ttggaaggca tgtaaagtac tcagacaatc
    16621 ttctgcgcgc aaaaaatttt gtatagtcgt cgttccttca gcaattgctc caaacatgat
    16681 gctgcgatgg gaaattgatt tatctgctgg tatcctaaca gtccctctta atccatgtat
    16741 ttgttgtaat aattgcattt atccagcctc ctatttgatc cagcaatgat aactggtatt
    16801 agacataatg attctttttg cttgtaacaa atcctgttgg tttttaaaac tgatttgcag
    16861 tattcctaaa atatcttccc tggtctcttg gatcttcaaa ttaatcaaag aaactcccgc
    16921 ttttccgatc agtccagtta cttctgcaat aacgccaggt tcatctggca catcgataaa
    16981 taaatcaaaa aaggcaggaa tcgcgccttg cttatggaca ggcaactggt ctcgactttc
    17041 ttttgcccga ttgaaaaaag aaaagatttc cgattggttc tcagtcatga tccattctgc
    17101 tatttgtgtc atttcttgtt gccaatctgc caatcgttcc aataatatct gcttattgct
    17161 caacaaaata tctgtccaca tgacaggatc tgaggaagcg atcctagtga tatctcgaaa
    17221 gcctcctgct gccagttgtt tggctcgcgg atactcttga ttgaattgtt ctgcctgatt
    17281 gactaagcca gctgccagaa tatgtggaaa atgacttagc attcctgtta tacggtcatg
    17341 ttctcttgct gtaagagtta catattttgc tttggttccg caaaatagtt cttgcagttt
    17401 ttcaacatct tttttcgtct tttccaaagg tgtaaagatg taataggcat tttcaaatag
    17461 tttagcatct gctgcctgta tacctgattt atgagaacca gccattggat gtccaccgac
    17521 aaaccgaaga tttttctgct cagcggatgt cacgacttgc tgcttcgtac tgctgacatc
    17581 cgtaatgatg acgtgatcag acaagttgat tctttcaagg acatcaagat aagctaaggt
    17641 tgtacggacg ggaacggcta ataagataac gtctgcttgt tctgcacctg tttcaaaatc
    17701 acttgcaacg taatcaacga tctgctgttc tactgcttgc tcacgggttt ttgactgact
    17761 atcccaacca actactatca gttctggatt ggtctgacga atacaaagtg ccatagaagc
    17821 accaatcagt ccaagcccca cgataaaaac atgttgtgcc atcttatttg cccccttttt
    17881 agtaatgttt caagtagtca cggtactcct tcactgcagc tttcaactca acaagcgaat
    17941 cacttgggaa ttttgacaat agctctgtcg ctaccacagt ggctacaaca ttttccgcaa
    18001 cgacgctagc tgcaggaact gctgtactat ccgaacgttc tacacttgct ttgtaaggtt
    18061 cttttgtatc gatatcaaca ctttgaagtg gcttgtacaa tgtaggaatg ggtttcatga
    18121 ccccgcgaat gacaagtggt tcgccattgg tcattccgcc ttcgaagccg cctagattat
    18181 tggtacgtcg gaaatagcct ctttcttctt cccataggat ctcatccatc acttctgagc
    18241 ctggtaatga accagaagtg aatccgaccc caaattctac gcctttaaag gcattgatac
    18301 taaccacagc ttgtgcgatt ttggcatcta gcttcgtgtc ccattgtacg tagcttccta
    18361 atccagctgg aacgcctcca acgagcactt ctacaattcc tccgatcgta tctccatttt
    18421 tctttgtctg atcgatcaaa tcttttattc tttgctcaac tgctggatca acaactcgta
    18481 cttctgacgc ttcagaaatt tctttgattt cttgaatcga gatatttcca ggtatttctg
    18541 cttggattcc accaagacaa atcacgtgtc ctgctacgat gatgtcaagt tcttccagaa
    18601 ttttcttggc aattgcgccg attgctacac gcatggtcgt ctctcttgca gaggaccgtt
    18661 caagcacatt ccgaagatct cgatgatggt atttcatacc gcctaccaaa tccgcgtgtc
    18721 ctggacgcgg acgtgcaact cgccgcagtg attggtcttt ttcaggcact tctgctacag
    18781 acatgacagt tgtccaattt ttccaatctt tattttctac cactaaagtg acaggtgaac
    18841 caattgtttt gccgtgacgg atgccagacg tgatccgaac acgatctttt tcgatcttca
    18901 tccttcctcc tctgccgtaa ccagcttgtc tgcgagcgag ttcgtgattg atttcttctt
    18961 ctgacagagg taatcctgct ggtagtcctt caataatggc tgttaattct ggtccatggg
    19021 attctcccgc agtcatataa cgcatcttac agtcctccta agtaatcttt gaatgtctct
    19081 gtgggaacag gaacgatttt tgcttgccca atcttttcta gcaagatgat attcaattgg
    19141 cttcctcttg ctttcttatc atgggttatc gcgtggaata attcatctgg atgacttaga
    19201 tcgtacgtaa tcggcagatg gaatttttga agtatccttt ctaaagcagc tgtcgatcca
    19261 gttggagaca agccgatttt ttctgcatga gaagtgatgg ctaccattcc aatagctact
    19321 ccttctccat gactgactac cccatatcct gctgtatttt caattgcatg gccaattgta
    19381 tgaccaaaat ttaaaataag tcgatttcct tggtcaaaga catcttcttc cactactttt
    19441 cgtttgattt ccaacgtagc agtaatgatt ggaacagcat gttccagaag atctttttcg
    19501 tctttcagct cgttcagctg tttccaaagc gttgcatctg ctatagctgc tgatttgatg
    19561 atttcagcga ttccttctcg taaacgacgt accggcaaag tttttagaac ctcaggatca
    19621 atcaatacac cagctggctg gctaaatgtt ccgacaaggt ttttcgcttt gtttgtattg
    19681 actgctgtct tcccgccaat actagaatct acttgtgcaa gtagcgtagt cggaatttga
    19741 acgaaatgga tcccacgcat gaacgtcgag gccacgaatg ctgctagatc acccaccatt
    19801 cctccaccaa gtgcaagtat cccatcgctg cgggtaaaat cattatctgc taaaaaatcg
    19861 tataactcag ctgcttgttc taaagattta ctggtctcac ccgccggtac gattgaaagg
    19921 actggttcaa aattagcttg tctcaattgg aacatgattt tttctgcata tagtggagca
    19981 acgtttgtat cagtgatcac agctactttt tgaggtctcc aaatcgtttg aagccatgat
    20041 ccagcctgtt ctagtgcgcc tttttcaata tgaatatcgt atttttggtt atcaacatgt
    20101 acagtaagca ttcgtttcac ttctttcttt taaaaagctg gaattaatcg agtgtctgaa
    20161 tcttttccat tgcttctttc atgatcgcaa cttctttcat catgcgtgag taagtctttt
    20221 cattcaacga ttggggaccg tctgaccaag catttgcagg atcaggatgg atctctacga
    20281 tcatgccatc tgctccgctt gccacacccg cacgagccat tggtggtaca agttcccaaa
    20341 ttcctgtacc atgacttgga tcaacaatca cagggaaatg agttaatttt ttcatcaatg
    20401 ggacagcact taaatcaaac gtattacgtg tcgctgtttc gtaagtccga atcccacgtt
    20461 cgataaaaat gacaggagat ttgtcttcga ctgcaatgta ttctgcagca ttaagccatt
    20521 cattgatcgt accggaaatt ccacgtttca atccaacagg tttacctgtt ttaccgacag
    20581 cagatagcag tttaaaattt tgcatattac gtgcgccaat ctgtaggata tcgctgtact
    20641 cagccaccat atcgatatgc ccttcatcca tcacttcagt aatcactttc attccaaatt
    20701 catcagccgc ttgacggatg tatttcaatc cttcttcttc taaaccttgg aaagcatatg
    20761 gagaagtacg tggtttgaag gcaccacctc gcagaatttt tgcgcccccg gctttggcca
    20821 ttcgcgcaca ttctctgatt tggtctaatc cttcgataga acaaggacca gccatcgtca
    20881 cgaaactgcc gtcaccaatt tttactccgt ctacatccac gactgtattg ccaggatgga
    20941 attctcgaga agtcaatttg tatgtgttta aaatacgcac agcattttct actccttcat
    21001 agcgtgtaaa tgctccttct tgtaaacttc gcgtatcacc ttttagtcca atcactactc
    21061 gttctgttcc ttgatcgatt gagactttga atccttcttt ttcgatatga tcgacgactt
    21121 tttttatttg ttcttccgtt gcttcttttt tcataattac tatcattgtt tttcccgctt
    21181 tcttttgttt acttatctat cttggctgat tttctctact ttttcaaggt ggttttacat
    21241 taattcctac taaatgaccg tgtcctgttg ttctcatttt cgctccttct ttcctactta
    21301 tgacccagat aatcaacaaa aaaagatgtc cctgtaggaa attgcctcta cagtgaacat
    21361 cttcaacgag ccttaaaaag cgctgcaaaa atataaaata gccactatag atgaatttct
    21421 ctacgtggct gtaagtcgtg tttaaaaatc tttagccatc atagatacaa acctggtttg
    21481 tttgtaccca cgaagtcatg aatacaaacc tatctaaaat agctaaagta atacgtattc
    21541 agttttgtaa gttttctaga tttcataaaa aatcgctcca aactgatgta agaattgtac
    21601 ctagtataga cataaaaaaa tcgactgtca atcattttta tgaaactttt tttaattttc
    21661 tgatagttaa ctttg
//
LOCUS       DACDKM010000038        21581 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20834_2016
            SAMN12640370-rid9891663.denovo.038, whole genome shotgun sequence.
ACCESSION   DACDKM010000038 DACDKM010000000
VERSION     DACDKM010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640370
            Sequence Read Archive: SRR10040891
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 21581)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21581)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742835.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742835.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:04:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,012
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 29 of 75
            Pseudo Genes (incomplete)         :: 33 of 75
            Pseudo Genes (internal stop)      :: 19 of 75
            Pseudo Genes (multiple problems)  :: 5 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21581
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640370-rid9891663.denovo.038"
                     /isolate="E20834_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..915
                     /locus_tag="IV334_001339"
     CDS             <1..915
                     /locus_tag="IV334_001339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002383715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAP8943418.1"
                     /translation="KQVAFNTYVGYKHYVHNRIYKFFYPKKIKLNRLKPYHLQEFYQY
                     MLNDNCTANTVIHYHAFIRKALQEAVITELISTNVADKVRKPKKKQFVSQVYNQQEIL
                     KLLDILSEEKLYLVVLLTAFYGLRRSEVLGLKWSAIDFLNKKIMINHVIIENPENMSQ
                     LIKKDQTKNSSSYRTLPLVETIERALVNQAKWQQDNRILLGDDYILEDNEYVFTMEDG
                     RLMKPQYVTHRLSKLIKKNGLKKIRFHDLRHSNASIMLDSGQNMKSIQEWLGHASYST
                     TANLYTHLTAGVKDRAAEALENAFGFKE"
     gene            1489..2058
                     /locus_tag="IV334_001340"
     CDS             1489..2058
                     /locus_tag="IV334_001340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1836 domain-containing protein"
                     /protein_id="HAP8943419.1"
                     /translation="MEKMRQQLMEWGKELETFRLPHWEELPDLELYMDQVRTLVDRYL
                     SPVIQGEKHPLLTSSMVNNYVKLGLIPAPVKKRYNKEHVAFLLAITTLKQVLTIPEIK
                     EGILFQGKTVGIREAYNLFCDEQEAAVWMVSQLAQGKSHPQKFDQATPVEYIAVRAAT
                     LSFAMKLLAEKTIVLETEYLKEEKTNEKQ"
     gene            2045..2905
                     /locus_tag="IV334_001341"
     CDS             2045..2905
                     /locus_tag="IV334_001341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HAP8943420.1"
                     /translation="MKNNKIAILVDSGTDVPEELIKKYEMYMIPLKIIYKDQVYTDKI
                     DITPEEIYQRLPEEIPGTSLPDGESITEIFEQIKKDGYEKVLAVTISSGLSGTYNVVR
                     LIAQQQEELEAYVLDTKNIGIGAGFSAIQAAKWLEEGVEWDQLISNLNELVERTKVFF
                     NVATLEYLQKGGRIGLVASIVGTALKLNPIISCNEEGIYYTVGKARGRKKSLDKTVAY
                     VKEKIGNAKVFNLAVAHGDAKEEAIEMEARLKREFPQANQIYFGQISPALVVHTGPGL
                     LGVGVQVLEG"
     gene            3095..3799
                     /locus_tag="IV334_001342"
     CDS             3095..3799
                     /locus_tag="IV334_001342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP8943421.1"
                     /translation="MKKVLVVDDEKPISDIVKFNLAKEGYDVYTAYDGEEALEKVAEV
                     EPDLILLDLMLPKMDGLEVAREVRKTYDMPIIMVTAKDSEIDKVLGLELGADDYVTKP
                     FSNRELVARVKANLRRGATAAKEPEEAAPAELTIGDLTIHPEAYMVTKRGETIELTHR
                     EFELLFYLAKHLGQVMTREHLLQTVWGYDYFGDVRTVDVTVRRLREKIEDNPSHPNYL
                     VTRRGVGYYLRNPEQE"
     gene            3804..5639
                     /gene="walK"
                     /locus_tag="IV334_001343"
     CDS             3804..5639
                     /gene="walK"
                     /locus_tag="IV334_001343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall metabolism sensor histidine kinase
                     WalK"
                     /protein_id="HAP8943422.1"
                     /translation="MKRKVRFFRSVNFKIAITFILILLISIEIIGAYFIRGLERSTIN
                     TFIKDMNQTVESLATTISPELNRKDNADDEEVNANIKRFIENSATSDIIEIRVVDEKG
                     IIRGTTDVNEQSAVGKKNDYVDINDFTTKRYVALDNDDKRVNINVQPILSPTGDAVIG
                     ALYVKSNIEQKYSEINNTAVIFFTASLIAAFISMIVSVLVARSITQPIGEMREQAIRI
                     ARGDYSRKVKVHGQDELGQLADTFNQLAERIEEAQDTMEAERNRLDSVLSHMTDGVIA
                     TDRRGKVITINDMAVSLLDIKNEEAIGQSILTLLDIEEEYTLRKLLEDPNEMVLDRST
                     STLESDQMILRVDFSMIRRESGFISGLVAVLHDVTEQEKTERERREFVSNVSHELRTP
                     LTSMRSYIEALSEGAWKDEEVAPNFLKVTLDETDRMIRMINDLLNLSRMDTGNTQLQL
                     EYVNFNELVNFVLDRFDMMVGNQEKNYKIRREFTQRDLWVEVDTDKIIQVVDNIMNNA
                     IKYSPDGGTITCRLLETHNNVILSITDQGLGIPKKDLNRVFERFYRVDKARARAQGGT
                     GLGLAISREVIKAHRGAIWAESKEGKGSTFYISLPYEPYEEEWWE"
     gene            5636..6952
                     /locus_tag="IV334_001344"
     CDS             5636..6952
                     /locus_tag="IV334_001344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8943423.1"
                     /translation="MRISEKVVRIGLILMVALSIYFSYAIWLSPAGKSSINVDETNTQ
                     MIESQSNRKTSEVFLPLHVTWHHSGMIQETNSENLVYRLQLIIDKARFGKLSEVVSGD
                     KSQFEQVKNLNSGFELAYNAPFLLREYKEAFDLDLDVSNLAKESENMYFTRIQFDQGN
                     KKIRFLNYSNHLVYEANISLNWTDINKELTAKDTKWTEMTTEPSTIDTQYATKHSIKL
                     KKYSYILSQQPYTLFRNAFFQKPDSVKNNDESNELIFYGGNETMSIHSETQVVDFRGE
                     LPEKKENASVFSESFPYISKLGSSLGNLRYFDRSDNTIDYRIFVEGFPVFGTDSRGQI
                     GVEVGGETNGSVQVNIQTSLNTIQVPIPSDEEVELASTNEIIQQLKAKGADSNKIGSV
                     IVGYTWQNVKETNRVVDLLPEWYVKYKDNWYSANDLLEKLPGLEAN"
     gene            6953..7822
                     /locus_tag="IV334_001345"
     CDS             6953..7822
                     /locus_tag="IV334_001345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8943424.1"
                     /translation="MDFKKIEWIFFVAFLGLNVFLFSSYHDAVYQENNVIRSNQTESI
                     QQRLASDDITYTGKISSENKQGYYLSAEQTNLSTSLANYRKAGKSGLLASGVFLDGDV
                     LTKSLSSSDAEKNVIDPDKISSSLEDFLSNKDNILFGNDYVYTKNFSHLEEEPLEIQA
                     SQEYEGIPFNDDTAKIVLSLEKNDNDYSITKYMQTHLSDIEQLREKTELYTEEEAINT
                     LYINNKIPRGSKILWRQLAYSCILKVREKNVYVPVWHVAIETSDKVVQIESVNAFSNT
                     IVTNNTVPKVEDQ"
     gene            7877..8686
                     /locus_tag="IV334_001346"
     CDS             7877..8686
                     /locus_tag="IV334_001346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HAP8943425.1"
                     /translation="MAEEKPAFKISILASGSSGNSLYIESEKKRLLVDAGLSGKKITS
                     LMAQIDRTPDQLDGILVTHEHRDHIHGVGVLARKYHLDVFANQQTWEAMEPLIGNVPV
                     EQKHLFEMGKVRTFGDIDIESFGVSHDAAAPQFYRFHKENRSFVMLTDTGYCSDYMRG
                     VIENADGYLMESNHDLEMLRMGPYPWNLKQRILGDRGHLSNEDGALVMTDVIGNRTKR
                     IYLGHLSKENNMKELAHLTMENVLKEKDFGVGHDFEIYDTDPDEATELFSI"
     gene            complement(8789..9418)
                     /locus_tag="IV334_001347"
     CDS             complement(8789..9418)
                     /locus_tag="IV334_001347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HAP8943426.1"
                     /translation="MKIALIGATGHAGSFILDEALSRDLDVTAIVRHPEKLPSDVPFI
                     QKDLFELTTTDLASFDVVVDAFNAPKGKEEMHQTSLSHLIEMLEGTDTRLIVVGGASS
                     LFLDTEKTTRMIDQVPKNAPFYPTAFNMYEGLLKLKQSKNLHWTYISPASLFDPHGKR
                     TGTYLLSDDYLQRNAASDSTISMADYAIALVDEILDEKHEKQHISVVSR"
     gene            9588..10880
                     /locus_tag="IV334_001348"
     CDS             9588..10880
                     /locus_tag="IV334_001348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAP8943427.1"
                     /translation="MKEWLDRAFLPMITSVVNLRPLTALRNGMTYTIPIIIVGSLFFL
                     FAHFPVDYIAQWIDKTGFSMYFTQVYQATFDVIAFWIVFAIAYAYAKEEDIDRLPVGI
                     TALSAYLLLIQPLNTQIDTVWTGAEGILGAVTTGLIIGWGYARLVRLAKGWKQREKIP
                     ENVWQSFTSLIPIIVIVTFTLIGSAVFQHLFRMTVVQGVWTYVQLPIQSFIDTLFGVL
                     LLGFFVPFLWLFGLHGSAMVNGLVSPVLQANSLANAEILASGQELTVANGGHIVTQQF
                     LDQFMTVTGAGLTLGAVFFMTVFAKSKKYRELGNLALLPAFFNINESIIFSTPIVMNP
                     MMAVPFIFAPVVSGLITYSALYFGFVPLFTAVQVPWTTPPILSGFLVGGVPAAILQGI
                     VLSISFFIYFPFLKKIDSANCKKERQTKNDGAAEKMND"
     gene            complement(10956..11618)
                     /locus_tag="IV334_001349"
     CDS             complement(10956..11618)
                     /locus_tag="IV334_001349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HAP8943428.1"
                     /translation="MSLKKYSFLSIFCYGLIFISPLILASIGLVKSTSELITATAVMY
                     ILGAVVLAIFYFKQREPLAIESAVKKASAPKIVIYGLVGIFVALILQSIAVMLESVLF
                     GKATPSENTQNIIQMIMEAPAFILATTVAGPIMEEFVFRRSILGIISRYSNFWVGAVI
                     SSLLFAFAHNDGHLLIYFFLGFFFSLEYKATGRIWTSMITHVGMNTLVVLVQLAVQKG
                     LV"
     gene            11843..12127
                     /locus_tag="IV334_001350"
     CDS             11843..12127
                     /locus_tag="IV334_001350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HAP8943429.1"
                     /translation="MLKPLGDRVIIEVAKEEEKTVGGIVLASAAKEKPQTGTVVAVGE
                     GRLLENGEKVPAAVKAGDQVMFEKYAGTEVKYEGKEYLIVAGKDIMAIVE"
     gene            12178..13803
                     /gene="groL"
                     /locus_tag="IV334_001351"
     CDS             12178..13803
                     /gene="groL"
                     /locus_tag="IV334_001351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736685.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HAP8943430.1"
                     /translation="MAKELKFAEDARAAMLRGVDKLADTVKVTLGPKGRNVVLEKSYG
                     SPLITNDGVTIAKEIELEDHFENMGAKLVSEVASKTNDIAGDGTTTATVLTQAIVREG
                     LKNVTAGANPLGIRRGIELATKAAVEELHNISTVVDSKEAIAQVAAVSSGSDKVGHLI
                     ADAMEKVGNDGVITIEESKGIETELDVVEGMQFDRGYLSQYMVTDNDKMEAVLENPYI
                     LITDKKISNIQDILPLLEQILQQSRPLLIIADDVDGEALPTLVLNKIRGTFNVVAVKA
                     PGFGDRRKAMLEDIAILTGGTVITDDLGLELKDATIENLGNASKVVVDKDNTTIVEGS
                     GEKEAIEARVQLIKNQIAETTSDFDREKLQERLAKLAGGVAVVKVGAATETELKELKL
                     RIEDALNATRAAVEEGMVSGGGTALVNVISKVSAVEAEGDVATGIKIVVRALEEPIRQ
                     IAENAGYEGSVIVDKLKNVELGTGFNAATGEWVNMVEAGIVDPTKVTRSALQNAASVS
                     ALLLTTEAVVADKPEPAAPAAPAMDPSMMGGMM"
     gene            complement(13892..15040)
                     /locus_tag="IV334_001352"
     CDS             complement(13892..15040)
                     /locus_tag="IV334_001352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAP8943431.1"
                     /translation="MLKVWVEQTKDGQFKFIERYVDPYTEKTRKKSTTLTSNSPQAWK
                     KAQKILDKKIKEALEDYNKSDITFGELYKEWYEYYKQHVKRTSYLKVPMMMKHVSKHI
                     SDDTIVRNIDETLINKIIEDMYTFGDLSLNYTKQTKTTLSVMLNYAIDRKYIQRNPAL
                     AVKIHPKKVEEEKRRLSMNKKYLEKEEIDQILKQLYSNPRRKLHGIIAEFLYLTGLRY
                     GELLALQMKDYEDGKISINGTLDYTSVKMDNAIKTTPKNTYSQREVQLPNRAKELIES
                     VIADNILAGRPTDPDQYIFISTSGTPLALHSFNAILHKVEEELELEKSLSSHIFRHSH
                     VSLLSELGVPLKAIMERVGHSDANTTLSIYNHVTKRAKQQVIDKLNSL"
     gene            complement(15142..15726)
                     /locus_tag="IV334_001353"
     CDS             complement(15142..15726)
                     /locus_tag="IV334_001353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4352 domain-containing protein"
                     /protein_id="HAP8943432.1"
                     /translation="MAKKVMGQDGKMYKVSKPFYKKVWFWVLAVILIIIIGSALNGGS
                     DSNKASDNGGEKVTKSSTSASSSKEEKSDTFYKIGDTVKVGDAEYTLNSVELTDERNQ
                     FEENQPAQVVKITYTVKNDGDSDIPVGTDVEVYGPDDKKSETYANENTMGSVAPGKQM
                     DVTAHFTLNQKGEIEIHFSPLVSFEKAAIFKATV"
     gene            complement(15853..16284)
                     /locus_tag="IV334_001354"
     CDS             complement(15853..16284)
                     /locus_tag="IV334_001354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HAP8943433.1"
                     /translation="MYLKLKEMLSEYNLKVVYMEMKEPGFYYPKPRIIFLNENLYGET
                     AEAFHLSHELAHFTASHFEYSVLYDTSTTFHSKFETEADKIAILILLNIYIENELTDE
                     SQFNLEKFMEYYSIQNKLRYTCYAVCQCYFKKKYSYARQYV"
     gene            complement(16302..16622)
                     /locus_tag="IV334_001355"
     CDS             complement(16302..16622)
                     /locus_tag="IV334_001355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP8943434.1"
                     /translation="MNTYEIIKELAKEKGLSIRQLEMNFGYSNGYLGSWKRQTPNSIE
                     LARLADYFGVSVDYLLGREERETPKHVDLSENDTVFSFDGKEISKETMRKAIAIAKAL
                     EENE"
     gene            17360..17617
                     /locus_tag="IV334_001356"
     CDS             17360..17617
                     /locus_tag="IV334_001356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase"
                     /protein_id="HAP8943435.1"
                     /translation="MNKEMSQTIKVQKMIDDLTHGIDSQADKIIKELQGQKVKDAKML
                     LKTINFEMNPTKRKLADVLEEKLASAINEQELLFETDTFNS"
     gene            complement(17588..17896)
                     /locus_tag="IV334_001357"
     CDS             complement(17588..17896)
                     /locus_tag="IV334_001357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8943436.1"
                     /translation="MKPEFVITMLDGEKIKIFADTLVVGYDDASNIEEKDGMFYSSQI
                     YVNTLQGDIGVSTLATSRPLVGLIGFLSKATFFSLGDDIDSDETVIYKTSAVKSIGFK
                     "
     gene            17974..18720
                     /locus_tag="IV334_001358"
     CDS             17974..18720
                     /locus_tag="IV334_001358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor"
                     /protein_id="HAP8943437.1"
                     /translation="MNTPQIFNFEQNEVRTILVNDEPYFVGKDVADVLGYSNPQKAIR
                     DHVDLEDKTQNDSFTVNGTAVVLINESGLYSLILKSKLPSAKKFKRWVTSEVLPTIRK
                     TGSYSNVPQSFAQALRLAADLEEKNQLLEQQIAEYEPKISYLDTILSSTDTVATSQIA
                     ADYGMSAIALNKLLNELGVQHKVSGQWILYRKHMNQGYTKSHTSEIPKADGGTKVVMN
                     TKWTQKGRVFIYNLLIAEGYYPQMDLEEIG"
     gene            18735..18938
                     /locus_tag="IV334_001359"
     CDS             18735..18938
                     /locus_tag="IV334_001359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP8943438.1"
                     /translation="MTDIAEITQRDREKIKEYVESSKFLTYTMLAERFGISKSYLSLI
                     LNGKKTSAEANRIIDSIITMYEL"
     gene            19043..19342
                     /locus_tag="IV334_001360"
     CDS             19043..19342
                     /locus_tag="IV334_001360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8943439.1"
                     /translation="MKKPTLSELIEAAEKAVKPDDWYRQSLILEKFHGMSKTTLVEYC
                     KEMETIPEFAEGILRPGHSTTFIHYHTFIWFLKWKDANKYRVKSLSPSDVLKEAS"
     gene            19342..19524
                     /locus_tag="IV334_001361"
     CDS             19342..19524
                     /locus_tag="IV334_001361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8943440.1"
                     /translation="MGKFNRALVFSAPLIIYALGLWGSRQALIGTIVYMVWIFMGLDE
                     AEHRAKKASREGLTKI"
     gene            complement(19530..20006)
                     /locus_tag="IV334_001362"
     CDS             complement(19530..20006)
                     /locus_tag="IV334_001362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8943441.1"
                     /translation="MRKKLNYILTATLTIYIILFITLSFLSSIKKIPFITLNKNSILK
                     EFNNTDYIAFLKLFSVCVLISCVIIFIVAKTCWTMLNKNQRDVENIAIFLIKITSSIS
                     IIIPMTVTFSKEQFNVAATYLSFIALFSFVLPKRNKSNFDNKELDEVRNSSPKEQK"
     gene            20059..20784
                     /locus_tag="IV334_001363"
     CDS             20059..20784
                     /locus_tag="IV334_001363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage regulatory protein"
                     /protein_id="HAP8943442.1"
                     /translation="MQELVILKNKEAVTTSLQVAESFKKKHKHVLEAIESIKRSVENS
                     ANVEDGSNFGQMFVEGNEPDSYGRSRRVYFMNRDGFSLLAMGFTGSKAINFKLKFIEA
                     FNEMEDVIRKNTVPQTIEDMMIYQLEEMKDVKKDVSMLKDTMRISGQQEFEIKQKGNM
                     KVMEVLGGKESRAYEEISKKVFSKFWSEFKRTFSIPRYGELPRKRFDDAVSFIEMWLP
                     ETAIRMEIDQLNRQQRLFGDENE"
     gene            20958..21299
                     /locus_tag="IV334_001364"
     CDS             20958..21299
                     /locus_tag="IV334_001364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8943443.1"
                     /translation="MNKELSTLDQYLTDSEWGKSNIKETNNRKIRRNLLTNEELSCDQ
                     DDLGNFVSIWDHVYLIHLSKHSNKPEYIYVIEDGLIDALEEYNRDNLIDISYYGSGKK
                     YIAEMEAEFDE"
     gene            21292..>21581
                     /locus_tag="IV334_001365"
     CDS             21292..>21581
                     /locus_tag="IV334_001365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1071 domain-containing protein"
                     /protein_id="HAP8943444.1"
                     /translation="MSEIKGTTNFEKLFSRKLNKILKKKGNFDYLSWAHAWEIMKKND
                     PQATVTINEYKHYRVVSGTHQDFLVEEYKPFLMDETGTYVSVSVTVKGHTET"
BASE COUNT         7310 a         3611 c         4325 g         6335 t
ORIGIN      
        1 aaacaagtag catttaacac atatgttggg tataagcatt acgtccataa tcgaatttac
       61 aagttttttt atcctaaaaa aattaaacta aataggttaa agccttatca tttacaagag
      121 ttttatcaat atatgttaaa tgataactgt acagcaaata cggttattca ttatcacgca
      181 tttatacgca aagctcttca agaggctgtt attacagaat tgataagtac aaatgtagca
      241 gataaagtaa gaaagccaaa aaagaaacaa tttgtttctc aagtttataa tcaacaagag
      301 atcttgaaat tacttgacat tttgtcagaa gaaaaactct atttggtagt tcttttaaca
      361 gcattttatg gattgcgaag aagtgaagtt ttaggattaa aatggtctgc tattgatttt
      421 ttaaacaaaa aaatcatgat aaaccatgta atcatagaaa atccagagaa tatgtcacag
      481 ttaataaaga aagatcaaac aaagaactct tccagctatc gtacattacc tttggttgaa
      541 acgattgaac gagcattagt aaatcaagct aaatggcaac aagataatcg tattctttta
      601 ggagatgatt atattttaga agataatgag tacgtattta ctatggaaga tggacggttg
      661 atgaagcctc aatatgtaac gcatcgtttg agtaagttga ttaagaaaaa tggattgaaa
      721 aaaattcgct ttcatgactt gcgtcattct aatgcttcaa taatgttaga tagtggccag
      781 aatatgaaat ccattcaaga atggctggga catgcatcgt attcgacaac tgctaattta
      841 tacacgcatt taaccgcagg agtaaaagat cgtgcagcag aagcattaga aaacgcattt
      901 gggtttaaag aataaaaaaa caaaaaacaa tatctttggg gtatcgcttc ttgtttttag
      961 aatttttttg ttggcaattt gttggcagta tgttggcaat gaaaaaattc acaaactaaa
     1021 aacctattta taaagcttta taaaactttt ttcacaaata gagcatttgg ttaagagtca
     1081 gctgctctac caactgagct aacggcccgt acaaaaagta cttaataata gtagcactaa
     1141 gaattttaaa atgcaagact acatttaaag aaaatcagaa aaatctttac ttccttatag
     1201 gaaatcattc atagattatt cacatttgct cgctattata taagcatacc aacaaacaaa
     1261 gttcttgcaa gagaacaaac cctaacactt ttttcatttt ttactccttt cccgccgtct
     1321 ccccggcggg tctcctgttc ttaacgataa aagacaagaa ggcttagcct tcttgttttt
     1381 tgttttcatg gaatcccgct aatcaaatca cctagttaaa gcaactagat tgaatgttta
     1441 tcataagtct gatatcatgt ttatgaaatg aaaaaaggag cggttatgat ggaaaaaatg
     1501 cgtcagcaat taatggagtg ggggaaggaa ctagaaacat tccgtctgcc tcactgggaa
     1561 gaactccctg acttagaact ttatatggac caagtcagaa cgttagttga tcgttatctt
     1621 tcacctgtga tacaaggaga aaagcatcca ttgcttacct cttctatggt aaacaattac
     1681 gttaaactag gtttgatacc agcaccagtt aaaaaaagat ataacaaaga acacgtagca
     1741 tttttacttg caatcactac gttgaaacaa gtattgacta ttcctgaaat caaagaaggg
     1801 atattgttcc aaggaaaaac agtaggaatt cgtgaagcct ataatctatt ttgcgatgaa
     1861 caagaagcag ctgtgtggat ggtcagccaa ttagcacaag gtaaatctca tccgcaaaaa
     1921 ttcgaccagg caacccctgt agaatatatt gctgtaagag cggcaacttt atcgtttgca
     1981 atgaaacttc ttgcggaaaa aacgattgtt ctagaaaccg aatatttaaa agaggagaaa
     2041 acaaatgaaa aacaataaaa tcgcaatttt agtagattca ggtacggatg ttccagaaga
     2101 attgataaaa aaatacgaga tgtatatgat cccattgaaa attatttata aagatcaggt
     2161 atatacagat aaaattgaca ttacccctga agagatctat caacgtttgc cagaggaaat
     2221 ccctggtaca tctttgccgg atggtgagtc gatcacagaa atctttgagc aaatcaaaaa
     2281 agacggttat gaaaaggtac ttgcggtaac aatttccagt ggattgagcg ggacatataa
     2341 tgtggttcgt ttaattgcac aacagcaaga agaattggaa gcatatgtac tagatacgaa
     2401 aaatattggt attggtgcag gtttttctgc gatccaagca gcaaaatggc tggaagaagg
     2461 agtagaatgg gatcaactga tcagtaatct gaatgagtta gtcgaaagaa caaaagtttt
     2521 ctttaatgtt gcgacattgg aatatcttca aaaaggtgga cggatcggtt tagttgcttc
     2581 tattgtagga accgctttaa agcttaaccc aatcatctca tgtaatgaag aagggattta
     2641 ttacacagta ggaaaagcta ggggacgtaa aaaaagtttg gataaaacgg ttgcttacgt
     2701 caaagaaaaa atcggtaatg caaaggtctt caacttagct gttgcccatg gggatgcgaa
     2761 agaagaagca attgaaatgg aagcgagact taaaagagag ttcccacaag caaaccaaat
     2821 ctactttggc cagatttctc cggcattagt agtccacact ggtccgggat tattgggtgt
     2881 cggtgtacaa gttttagaag gataaatcaa aaaaaggaaa cactgaggtg aaaatcagtg
     2941 ttttcctttt ttattgaagt tagaacaaaa aataataaag tattttgcac tttcatttta
     3001 attttctctt ttgttttata ggcaaagatg aaacaaaagc agaaatacgg tataatgaga
     3061 aggtatgaca ataatatata agaggtggct ctttatgaaa aaagttttag ttgtcgatga
     3121 tgagaagccg atttcggata tcgtcaaatt caatttagcc aaagaaggct atgacgttta
     3181 tactgcatat gatggggaag aagccttgga aaaagttgct gaggtagaac ctgatttgat
     3241 tttacttgat ttgatgctac ctaaaatgga cggcttagaa gttgctcgtg aggtacgcaa
     3301 aacttatgat atgccgatca ttatggtaac agccaaagat tctgaaattg ataaagtcct
     3361 aggcttagag ttaggcgccg atgattacgt gaccaagcca ttttccaacc gagaattagt
     3421 tgcacgtgtc aaagcaaatc ttcgtcgcgg tgccacggct gcgaaggaac cagaagaggc
     3481 agcacctgcg gaattgacga ttggtgattt gacgatccat ccagaagctt acatggtgac
     3541 gaaacgagga gaaacaatcg agttaacaca tcgtgaattc gaactactct tttacttggc
     3601 gaagcacctt ggacaagtga tgacaagaga acatttatta caaacggttt ggggttatga
     3661 ttattttgga gatgtacgaa cagtggatgt aactgtacgt cgtttgcgtg aaaagatcga
     3721 agataatcca agccatccaa attatttagt gacacgtcgt ggtgtcggtt actatttgcg
     3781 taacccagaa caggagtaat tggatgaaaa gaaaagtccg cttttttcga tcagtcaatt
     3841 ttaagatcgc tattaccttt attctgattt tattgatctc gatcgaaatt attggtgcct
     3901 actttatacg tggtttagaa cgttccacga tcaatacatt tattaaagat atgaatcaaa
     3961 cggtggaatc attagcaaca acaatcagcc cggaattgaa tcgaaaagat aatgcagatg
     4021 atgaagaggt aaatgcgaat atcaaaagat ttattgaaaa cagtgcgact agcgatatca
     4081 tcgaaatacg ggtcgttgac gaaaaaggga tcatccgagg aacaaccgat gtcaatgaac
     4141 aaagcgctgt gggaaagaaa aatgattatg ttgatatcaa tgatttcacg actaaacgtt
     4201 atgttgcttt agataacgac gataaacggg tgaatatcaa tgttcaaccg atcctgtccc
     4261 cgaccggcga tgccgttatc ggggctcttt acgtcaaaag taatatcgaa caaaaatatt
     4321 cggaaatcaa taacacagca gtcattttct ttactgcctc actaattgcg gcatttattt
     4381 caatgatcgt atctgtttta gttgctcgtt cgattacaca gccgatcggt gaaatgagag
     4441 aacaagccat tcgaatcgct agaggagact acagtcggaa agtcaaagtg catggacaag
     4501 atgaacttgg acagctggct gataccttca atcagttagc tgaacgtatt gaagaagctc
     4561 aagatacaat ggaagctgaa cgcaatcgtt tggatagcgt gctatcccat atgacagacg
     4621 gcgtcattgc gacagatcgc aggggtaaag tcattacgat caatgatatg gctgtttcct
     4681 tgctagatat caaaaacgag gaagcaatcg gacaatcgat tttgacactt ttagatatcg
     4741 aagaagaata tacgttgcgg aaattattag aagatccaaa cgaaatggta cttgaccgtt
     4801 caaccagtac attggaaagt gatcagatga tcttgcgagt agacttttct atgatccgtc
     4861 gcgaatcagg ttttatcagc ggattagtag ctgtcttgca tgatgtgaca gaacaagaaa
     4921 aaacagaacg ggaacgtcgt gaattcgtct ctaatgtttc ccatgaactt cgtacaccgt
     4981 tgacaagtat gcgtagttat atcgaagcat tgagcgaagg tgcgtggaaa gacgaggaag
     5041 tagcaccaaa ttttttgaag gtaacgctag atgaaacaga ccgaatgatt cgaatgatca
     5101 atgacctctt aaatctatct cgcatggata caggtaatac ccagcttcaa ttagaatatg
     5161 tcaatttcaa cgagttagtg aattttgttc ttgaccggtt tgatatgatg gtgggaaatc
     5221 aggaaaaaaa ttataagatc cgtcgtgaat ttacgcaaag agatctttgg gtcgaagtag
     5281 atacagataa aatcatccaa gtagtcgata atatcatgaa taacgcgatc aagtattcac
     5341 cagatggggg aacgattact tgtcgcttat tagaaacgca taataacgtg attttaagta
     5401 tcaccgatca aggattgggg attcctaaaa aagatctgaa ccgagtgttc gagcggttct
     5461 atcgagtaga taaagcacgt gcaagagcac aaggtgggac aggacttgga ttagctattt
     5521 ctagagaagt gattaaggcg catcgaggcg ctatttgggc agagagcaag gaaggaaaag
     5581 ggtcgacttt ttatatctcg ctgccatatg aaccttatga ggaggaatgg tgggaatgag
     5641 aatctctgaa aaagtcgttc gtataggact cattttgatg gttgcgttaa gcatatactt
     5701 ctcttatgca atctggctga gcccagcagg aaaaagttca atcaacgtag atgagacaaa
     5761 cactcaaatg atcgagtctc aaagcaatcg aaaaacttcc gaagtctttc ttccgcttca
     5821 tgttacatgg caccattcgg gaatgatcca agaaaccaac agtgaaaatc tcgtgtaccg
     5881 attgcaattg atcatcgata aagcaagatt tggaaagctt tctgaagtag tttcaggaga
     5941 caaatcacag tttgaacagg tgaaaaatct gaactctggt tttgagttgg cctataatgc
     6001 gccttttttg ctaagagaat acaaggaagc atttgattta gatttagatg tgtcgaactt
     6061 ggcaaaagaa agcgaaaata tgtattttac tagaatacaa ttcgatcaag gaaataaaaa
     6121 aatccgattt ttgaattatt cgaaccatct cgtatatgaa gcaaatattt cgttgaattg
     6181 gacagatatc aataaagaat taacagcaaa agacacaaaa tggacagaaa tgacaacaga
     6241 accatcgacg atcgacacgc aatatgctac gaaacattcg atcaaactga agaagtatag
     6301 ttatatcctg tcacagcaac cctatacact ttttagaaat gcttttttcc agaaaccaga
     6361 tagcgtgaaa aataatgatg aaagcaatga attgattttt tacggtggga atgaaacaat
     6421 gtctatccat tcggaaacgc aagtagttga ttttcgtgga gagctgccag agaaaaaaga
     6481 aaatgctagt gtattttctg aaagtttccc ctatattagc aaattaggca gttctttagg
     6541 gaacctcaga tattttgacc gcagtgacaa tacgatcgat tatcggatat tcgtggaagg
     6601 cttcccggtt tttgggacag acagcagagg acagattggt gtggaagttg gcggtgaaac
     6661 gaatggttct gtgcaagtga atatccaaac cagtttgaat acgattcaag tgccgatacc
     6721 ttcagacgaa gaagtagaac ttgcaagtac gaatgaaatc atccagcagt taaaagctaa
     6781 gggagcagat agcaacaaga ttggttcagt gatcgttggc tatacatggc aaaatgtcaa
     6841 agaaacgaat cgtgtcgttg atctcttacc tgaatggtat gtgaaatata aagataactg
     6901 gtattcagct aacgaccttc tggaaaaatt acctggattg gaggcgaatt aattggattt
     6961 caaaaaaatc gaatggatat ttttcgtcgc ttttttaggg ttgaatgtgt ttttattcag
     7021 cagttatcat gacgcggtat accaagaaaa caatgtcatt cgctctaatc agactgaatc
     7081 aatccaacaa cgtctggcta gcgacgatat cacttacacg gggaagattt cgtctgagaa
     7141 caagcaaggc tattatctta gcgcggagca gaccaatctt agcacttcat tagcgaatta
     7201 tcgcaaggct ggtaaaagcg gcttgctggc aagtggtgtg tttttagacg gtgatgtttt
     7261 gacgaaatct ttatccagtt cagatgctga aaagaacgtg atcgatccag ataaaattag
     7321 tagttccttg gaagattttt tatctaacaa agacaatatt ttattcggta atgattatgt
     7381 gtacacgaaa aacttttctc atttagaaga agaaccacta gaaatccaag caagccaaga
     7441 gtatgaaggc atcccattca atgatgatac agcaaaaatc gttctttctt tagaaaaaaa
     7501 cgataatgat tatagcatca cgaaatacat gcagacgcat ttatctgata tcgagcagct
     7561 gcgcgaaaag acggaactgt atacggaaga ggaagcaatc aatacattgt acatcaataa
     7621 caaaatacca agaggatcaa aaattttatg gcgtcagcta gcttattcct gtattctgaa
     7681 agtccgggaa aaaaatgtgt atgttcctgt ttggcatgta gcgatcgaaa caagcgataa
     7741 agtggtccaa attgagagcg tcaacgcttt cagcaatacg atcgtgacaa acaatactgt
     7801 accaaaagtg gaagatcaat aaaaaagcga gtataatgaa aaaagtttac aaagtaagaa
     7861 gggaagtagg agtcaaatgg ctgaagaaaa accagctttt aaaatcagta tcctagcaag
     7921 tggaagttcg ggtaattcac tttatatcga aagtgaaaaa aaacggcttt tggtcgatgc
     7981 aggacttagc ggtaaaaaaa tcacttcctt gatggctcaa attgatcgga caccggacca
     8041 attagatgga atacttgtga cgcatgaaca tcgagatcat atacatggcg tgggtgtctt
     8101 ggctcgtaag tatcatttgg atgtttttgc caatcagcag acttgggaag cgatggaacc
     8161 actgattgga aatgtaccag tggaacaaaa acatctattt gagatgggga aagtcagaac
     8221 attcggcgat atcgatattg agagtttcgg cgtctcccat gacgcagcgg ctccgcaatt
     8281 ttatcgtttc cataaagaaa atcggtcatt cgtcatgttg acggacaccg gatattgcag
     8341 tgattatatg cgaggtgtca ttgaaaatgc cgatggttat ttgatggaaa gtaatcatga
     8401 tcttgagatg ttacgcatgg gaccttatcc gtggaattta aaacagcgta ttttaggaga
     8461 tagaggacat ctttccaacg aagacggtgc acttgtcatg actgatgtga tcggtaatcg
     8521 gaccaaacga atctatcttg gccatcttag caaggaaaac aatatgaaag aacttgctca
     8581 tttaacaatg gaaaatgttt tgaaagaaaa agattttggc gttggacatg attttgaaat
     8641 ctatgataca gatccagatg aagcaactga gctattttcg atttaaacag ttcaatcaat
     8701 aaaaatataa aaagcagaag ataaaatcaa tcagggttgg ttttatcttc tatttttatt
     8761 tttttagcat attaaaaaaa taaccgtatt atcgactgac tacactgatg tgctgctttt
     8821 catgtttctc atccagtatc tcatctacta atgcaatcgc gtaatctgcc atgctgatcg
     8881 tactgtcgct agcagcgttt ctttgtaaat aatcatcact taacaaataa gtacctgtac
     8941 gtttgccatg tggatcaaat aaagaagctg gactgatata agtccaatgt agatttttag
     9001 attgtttcaa ttttaacaat ccttcgtaca tattgaaagc tgttggatag aagggtgcat
     9061 ttttaggtac ttggtcaatc atacgagtcg tcttttctgt atctaagaat aacgaagagg
     9121 cacccccaac gacaattaag cgtgtatcgg ttccttcaag catctcaatc aaatgggaca
     9181 aagaagtttg atgcatttct tcttttcctt tgggagcgtt gaaagcatca acgacaacat
     9241 caaaagatgc aagatcagta gttgtcaact caaataaatc cttttgtata aacggaacat
     9301 ctgatggcaa tttttctgga tgacgcacga ttgccgtcac gtctaaatct ctggacaatg
     9361 cttcgtctaa aataaatgaa cctgcatggc cagtagctcc aatgagtgca attttcataa
     9421 aaaacctcct tttttattcc tgtccattgt aagcagttgc tgaatattcg tcaaagaatc
     9481 cgatttttga aaagctcagt gcgcattcct ttcatggttc gtgatcattg ggtatagtta
     9541 aattaagaaa actgattgat aaaaatagaa gttgaaggag ataagaaatg aaagagtggt
     9601 tagatcgtgc ctttttacca atgataacat ctgttgtgaa tcttagaccc ttaactgctt
     9661 tgcgtaatgg aatgacttac acgatcccaa taattattgt tggctcgctg tttttcttat
     9721 ttgctcattt tcctgtggat tacatcgctc agtggataga caaaacagga ttttctatgt
     9781 attttacaca agtataccag gctacatttg atgtgatagc cttttggatc gtttttgcca
     9841 ttgcatacgc ttatgcaaaa gaagaagaca tcgatcgctt accagtcggc ataacagcct
     9901 tgtctgctta tctactgctt attcagccac taaatactca gatagatact gtctggacag
     9961 gtgctgaagg gatactagga gcagttacaa ctgggctcat catcggatgg ggctatgcac
    10021 gtttagtaag acttgcgaaa gggtggaagc aaagagagaa gatacctgaa aatgtttggc
    10081 agtcttttac ttcattgatt ccaatcattg ttatagtcac ttttacgctt atcggttcag
    10141 cggtttttca acatttgttc agaatgactg ttgttcaagg agtatggaca tatgtccagc
    10201 ttcccattca atcgttcata gacacgttgt ttggtgtgct tttattaggt ttttttgttc
    10261 cgtttttatg gttatttggt cttcatggat ctgcgatggt caatgggttg gttagtcccg
    10321 tcttgcaagc gaattcatta gcaaatgcag aaatcttagc ctcaggacaa gagttgacgg
    10381 tggcaaatgg cggacatatc gttactcagc aatttctcga tcagttcatg acggttacgg
    10441 gtgctggatt gacgttaggg gctgtatttt ttatgacggt ttttgcgaaa tcgaagaaat
    10501 acagagaatt aggaaatctt gctcttttac cagccttttt caacatcaat gaatcaatta
    10561 ttttttccac accgatcgtg atgaacccga tgatggctgt tccttttatt ttcgcaccgg
    10621 tcgtttcagg attgattacc tattccgctt tatattttgg attcgttcct ttatttacag
    10681 cagtccaagt cccttggaca acaccgccga tcctctcggg tttcttagta ggcggagtgc
    10741 ctgcagcgat tttacaaggt atcgtgttgt caattagttt tttcatttat ttcccatttt
    10801 tgaaaaaaat agattctgca aactgcaaaa aggaaagaca gacaaaaaat gatggagcag
    10861 cagaaaagat gaacgactaa aaaacaaaaa tactcctgtc gctttagatt gggaaaatca
    10921 ccaatctagg atgacaggag tatttatagt catgtttata ctagcccttt ttggacagct
    10981 aattgaacaa gaacaactaa tgtgttcatt cctacgtgtg tgatcatcga tgtccagatc
    11041 cgaccagttg ctttgtactc taggctgaag aagaaaccta ggaaaaagta gattagcaga
    11101 tgaccatcat tatgagcaaa cgcaaacagc aaagagctga tcactgctcc gacccaaaaa
    11161 tttgaatatc gactgatgat ccccagtata ctgcggcgga atacgaactc ttccatgatg
    11221 ggtccagcaa cagttgtcgc caaaataaaa gcgggtgctt ccatgatcat ctggatgata
    11281 ttttgtgtat tctcggaagg ggttgctttg ccaaacaata cagattctaa catgacagcg
    11341 atgctttgca gaatcaatgc gacaaagatt ccaaccaatc cataaataac aatctttgga
    11401 gcagaagctt tttttaccgc tgattcgatc gctaagggtt cacgttgctt aaaataaaaa
    11461 atagctagga caacggcgcc tagtatatac atgactgcag ttgctgtgat caattcagac
    11521 gtagatttta caagtcctat cgaagctagg atcaagggtg agataaaaat tagaccatag
    11581 caaaaaatgc tgaggaagct atattttttt aaagacatgt aattcctcct tttaagggat
    11641 aacaagcatg caaggcaagc ttatatcgat tgatttcaat tatatcataa tttcaataag
    11701 cagctgtcag aataaaattt cttttctttg atttaaaagc ttgcaaaaaa gagggagaat
    11761 gagtattatt aaaaatgtag gttagcactc gaaggttaag agtgctaaca gattaaaaaa
    11821 agagttatcg gagggattta tcgtgttaaa accattaggt gatcgcgtca ttatcgaagt
    11881 cgcaaaagaa gaagaaaaaa ctgtcggagg aatcgtttta gcttctgctg ctaaagagaa
    11941 accccaaact gggacagttg ttgcagtcgg tgaaggccgt ttgttagaaa acggtgaaaa
    12001 agttcctgcc gctgtaaaag caggcgacca agttatgttc gaaaaatacg ccggtacaga
    12061 agtaaaatac gaaggaaaag aatatctgat cgtcgcagga aaagatatca tggcgattgt
    12121 tgagtaaatt tggaagataa aaaaataatc aaaaataatc aatgaggtga atcaatcatg
    12181 gcaaaagaat tgaaatttgc agaagacgca cgcgcagcta tgttacgcgg ggtagataaa
    12241 ttagcagata cagtgaaagt aacattagga cctaaaggcc gaaatgtcgt actagaaaaa
    12301 tcttacggct ctccattgat cacaaatgat ggtgtgacga tcgcaaaaga aatcgaatta
    12361 gaagatcatt ttgaaaacat gggtgcaaaa cttgtatcag aagtagcatc aaaaacaaat
    12421 gatattgcag gagacggtac aacgacagca actgttctga cacaagcaat cgttcgcgaa
    12481 ggtttaaaaa acgtaactgc aggtgctaat cctctaggca ttcgtcgcgg gatcgaattg
    12541 gcaacaaaag cagcagtcga agaattgcat aatatctcaa ctgttgttga ttcaaaagaa
    12601 gccattgcac aagtagcagc tgtttcttcg ggttctgata aagttggaca tttgattgct
    12661 gatgcaatgg aaaaagttgg aaacgacggt gtcatcacaa tcgaagaatc aaaagggatc
    12721 gaaacagaac tagatgttgt agaaggtatg cagtttgatc gtggttactt atcacaatat
    12781 atggtaacag ataacgacaa aatggaagct gttctagaaa acccatatat cttgatcaca
    12841 gacaaaaaaa tctctaacat ccaagatatt ctgccattgt tagaacaaat cttgcaacaa
    12901 tcacgtccat tgttgattat tgctgacgat gtagatggtg aagcattgcc aactcttgta
    12961 ttgaacaaaa tccgtggaac attcaacgta gtagctgtaa aagctcctgg tttcggcgat
    13021 cgtcgtaaag caatgcttga agatatcgct attttgacag gtggtacagt aattactgac
    13081 gatctaggtt tagaattgaa agatgcaaca atcgaaaact taggaaatgc ttctaaagtt
    13141 gttgtagata aagacaatac aacgatcgta gaaggttctg gagaaaaaga agcaatcgaa
    13201 gctcgtgtgc aattgatcaa aaaccaaatt gctgaaacaa cttctgattt tgaccgcgaa
    13261 aaattacaag aacgcttagc taaattagca ggtggtgtag ctgttgttaa agttggtgca
    13321 gctactgaaa cagaattgaa agaattaaaa ttacgcattg aagatgcatt gaatgctaca
    13381 cgtgccgcag tagaagaagg aatggtctca ggtggtggta cagctctagt gaacgttatc
    13441 agcaaagttt ctgctgtaga agcagaagga gacgtggcaa caggtatcaa gatcgttgtt
    13501 cgtgctttag aagaaccaat tcgtcaaatc gctgaaaacg ctggttatga aggatcagtg
    13561 attgttgata aattgaagaa cgtggaacta ggcactggtt tcaatgctgc tactggagaa
    13621 tgggtaaaca tggttgaagc aggtatcgta gacccaacta aagtaacacg ttctgccttg
    13681 caaaatgccg cttccgtttc tgccttgtta ttaacgacag aagcagtcgt tgcagataaa
    13741 ccagaaccag cagcgccagc agctcctgca atggatccat caatgatggg cggtatgatg
    13801 tagtaattaa gaaagaaccc tattttaaca gggttctttc ttcttttttt gttttatttt
    13861 aagcaaaagg ggcaaagaag gggcaaaaat atcaaaggct atttagttta tcaattactt
    13921 gttgtttggc tctttttgtt acatggttat aaatagacag agttgtgttt gcatcagaat
    13981 gccctacacg ctccataatg gctttaagag gtacgcctaa ttcagataat agtgaaacgt
    14041 ggctatgtct gaatatatgt gaggatagac ttttttctaa ttccaactct tcctctactt
    14101 tatggagtat agcgttaaat gagtgcagcg caagcggagt gccacttgta gatataaata
    14161 tatattgatc gggatctgtg ggtctacctg caagaatatt gtcagctatc acactttcaa
    14221 tcaattcttt tgcacgattg ggtaattgca cttcacgttg cgaataagta ttttttggag
    14281 ttgtttttat agcattatcc attttcacag atgtgtaatc taaggtccca ttaatggaaa
    14341 ttttcccatc ctcatagtcc ttcatttgca aagctagcaa ttccccatat ctcaaaccag
    14401 ttaaatatag aaactcagct attatgccgt gtagttttct gcgaggattg gagtatagct
    14461 gtttcagtat ttgatcaatt tcttctttct ccagatattt tttattcata gaaagccttc
    14521 ttttttcttc ttccactttt ttaggatgga ttttaactgc tagcgcaggg tttctttgaa
    14581 tgtattttct atcgattgca tagtttagca taacagatag agttgttttt gtttgttttg
    14641 tgtagttcag tgagaggtca ccaaacgtat acatatcttc aattatctta ttaatgagtg
    14701 tctcatcaat gtttctaacg atcgtatcat cgcttatgtg cttagaaaca tgtttcatca
    14761 tcattggaac cttcaaatag ctagtacgtt taacatgctg cttataatat tcataccatt
    14821 ctttatacag ctcaccaaaa gtgatatctg atttattgta atcttcgagt gcttctttaa
    14881 tttttttatc tagaattttc tgagcttttt tccacgcttg tggtgaatta cttgtaagtg
    14941 ttgtagattt ttttcgtgtt ttttctgtat aaggatctac atatctttca ataaacttga
    15001 attggccatc tttggtctgt tcaacccaca cttttaacat ctcctatcat ttgctataat
    15061 aggcataaca aatagaccta tataggtttg ttttctaaaa gcacgctctt actctggacg
    15121 gtggggcgtg ttttttattt attatacagt tgctttaaaa atagctgctt tttcaaatga
    15181 tactaaagga gagaagtgga tttctatttc tcccttttgg tttaacgtga aatgagcagt
    15241 tacatccatt tgttttcctg gagcaacaga tcccattgta ttttcgttgg catatgtctc
    15301 tgattttttg tcatctggtc catatacttc cacatctgta cctacaggga tatctgaatc
    15361 gccatcattt tttacggtat aagtaatttt tactacttgt gcaggttggt tttcttcaaa
    15421 ttgatttctt tcatcagtta gttctacact atttagtgta tattcagcat caccaacttt
    15481 tacagtgtca ccaatcttat agaaggtatc gcttttttct tctttagaag atgaagcaga
    15541 ggttgaagat ttagttactt tttcgccacc attatcactt gctttattac tatctgatcc
    15601 accatttaaa gcagaaccaa taattataat taaaattact gctaataccc aaaaccatac
    15661 ttttttgtaa aaaggtttac taactttata catttttccg tcttgaccca taactttttt
    15721 tgccatttaa atattcctca cttcttgtta taatatattt gcgatctcag aaatgaggta
    15781 tgagtccgtg tgccagcacg ggctttttta atgttttgct gaaatcggtt tttttaataa
    15841 ttcttgatgt attcatacat attgccttgc gtaagaatat tttttcttaa aatagcattg
    15901 gcagacagca taacaagtgt atctgagctt attctgaata gaataatact ccataaattt
    15961 ttcgagatta aactgagatt catcagtcag ttcgttctca atatagatat ttaataaaat
    16021 tagaatagct attttatctg cttctgtttc aaattttgag tgaaaagttg tagaagtatc
    16081 gtacaaaact gaatattcaa aatgagaagc ggtgaaatgc gcaagttcat gagataaatg
    16141 aaaggcttct gcagtttcgc catatagatt ttcattcaaa aaaatgattc taggcttcgg
    16201 ataatagaag ccaggctctt tcatttccat gtaaactact ttcaaattgt attcgctcaa
    16261 catttctttc aatttcaaat acatacaacc catcactcca actattcatt ttcctctaaa
    16321 gctttagcaa ttgcaatcgc tttacgcatt gtctccttag atatttcttt tccatcaaaa
    16381 gaaaaaacag tatcgttttc tgataaatcc acatgtttag gggtttctct ttcttctcta
    16441 cctagaaggt agtctacaga gactccaaag taatcagcta accgagctaa ctcaatagaa
    16501 ttaggagttt gtcttttcca acttcctaaa tatccattcg aatacccgaa attcatttcg
    16561 agctgtctaa tggataatcc tttttctttt gctaactcct ttattatttc gtaagtattc
    16621 attgataaat caacctttct gaatgcttac aaaaaaagtt tagataaata atgtaaaaat
    16681 agttgactta tatacattat taatctatac tatgttttgt aaacaagttt aacaactaaa
    16741 aagacaacaa aaaatactat tgattaataa atgccaaccg ccaagaaagc tttttaaatc
    16801 aaagtttata tgtcttattt aactatgcat ttagtataga ttattaatct aataaagtca
    16861 acgcagtttt ttaaaaagtt gttaaatttg tttacgaata taaaagaaag gagagaagaa
    16921 tatggaaaaa acagtctcgg caaaaatcga agatttgaaa ttagacatac tgaaacaagc
    16981 aaaagtggcg atggaacacg cggtagataa agaagactct gccatggttg cagccatagc
    17041 agagatttta gctcacgttt agtcatcgtt tttacggtct tctggaagct gaccatagat
    17101 aacagcataa tgaatatcta aataagcttg aacaatttct tttggcgaaa ttgattcgcc
    17161 tttagtagat acttgtgatt cgtgatttag ccatgcaaca actaaatccg cagcgatttc
    17221 aaacggcgtt acactttgtg tcagttgcca taaagaattt cacaatatgt atggtcgtgg
    17281 taataatacc aaagagcaat ttgaggaatt tatggaaatt aaaaaataca aaaaatcatt
    17341 tattaaagga ggctaaacaa tgaacaagga aatgagccaa acaatcaagg tacaaaaaat
    17401 gatagatgat ctaacacatg gaattgatag ccaagccgat aaaatcatca aggaattgca
    17461 aggacaaaaa gttaaggatg caaaaatgct tctcaagact atcaactttg aaatgaatcc
    17521 aacaaaaaga aagctcgccg atgtgttgga agaaaagttg gcttccgcta tcaacgagca
    17581 agaattacta tttgaaaccg atacttttaa cagctgatgt tttatagatg acagtttcat
    17641 ccgaatctat atcatcgccg agagaaaaga atgttgcttt tgacaaaaag ccgataagac
    17701 ctacaagcgg tcttgatgtt gcaagagtag ataccccaat atcaccttgc aaggtattca
    17761 cgtaaatttg agaagaatag aacattccat ctttttcttc gatatttgac gcatcatcgt
    17821 atccaacgac caaagtatcg gcaaatattt tgattttttc tccatcaagc atagtaatta
    17881 caaattctgg tttcataaat tttcacctcc ttaacaatta tttcagcctg tcacactgat
    17941 aaggaaatta taccaaagaa aggaatgaga aatatgaata caccacaaat ttttaatttc
    18001 gagcaaaacg aagttcgaac tattttagta aatgatgaac catattttgt aggaaaagat
    18061 gtagcagacg ttctaggata ttcgaatccg caaaaagcta ttcgagatca tgtggacttg
    18121 gaggataaga cgcagaacga ttcgttcacc gtcaatggaa cagcagttgt tttgattaac
    18181 gagtcaggcc tttacagttt aatcttaaaa tcaaaacttc cctctgccaa aaaatttaaa
    18241 cgttgggtaa cgagtgaagt ccttccaaca attagaaaaa caggtagcta ttcaaacgta
    18301 cctcaaagtt ttgcacaagc attgcgttta gcagcggatt tagaagaaaa gaaccaatta
    18361 ctcgaacaac aaattgccga gtacgaacca aagattagct acttagatac gattctttca
    18421 tcgacagata cggtagctac ttctcaaatt gcagctgatt acggaatgtc ggcaattgct
    18481 ctaaacaaat tgctaaacga gttaggtgtt cagcataaag ttagcggaca atggatactt
    18541 taccgaaaac atatgaacca aggatacaca aaatcgcaca caagtgagat accgaaagcc
    18601 gatggcggca ctaaagttgt aatgaatacc aaatggacac agaaagggcg agtgtttatt
    18661 tacaacttat taatcgcaga gggctattac cctcaaatgg atttagagga aattggttag
    18721 aaaggagttt tagtatgact gacattgcag aaatcactca acgagataga gaaaaaatca
    18781 aagaatatgt cgaaagttca aagttcttaa cttacaccat gcttgctgaa agatttggaa
    18841 ttagcaaaag ctacttatct ttaattttaa acggtaaaaa gacttctgca gaagcaaaca
    18901 gaattataga ttcgattatc actatgtacg aattgtagag ggaggtaaag tcgttggaag
    18961 ataaaatcat tcttacaaaa tcagagcttc aagaaatgtt aaacatcgag tatggaacga
    19021 taatttttag gagggataat aagtgaaaaa accaacactt tcagagctga tagaagctgc
    19081 tgagaaggca gtaaaaccag acgactggta ccgacaaagt ttaatcttgg agaagttcca
    19141 cggcatgtca aaaactactt tagttgaata ctgcaaggaa atggaaacaa ttcctgaatt
    19201 tgcagaagga atacttcgtc caggtcactc aaccacattt attcattacc atacttttat
    19261 ttggttttta aagtggaaag acgcaaacaa atatcgtgta aaaagtttat cgccttcaga
    19321 tgttttgaag gaggcaagtt aatgggcaaa ttcaacagag cactagtatt cagcgcaccg
    19381 ctaatcatct acgctttagg cctctgggga agcagacaag cgttgatagg gacaatcgtt
    19441 tacatggttt ggatttttat ggggcttgat gaagctgagc acagagcgaa aaaagccagt
    19501 cgggagggac tgactaaaat atgatttttt tatttttgtt cctttgggct gctattgcgt
    19561 acttcatcca gctccttgtt gtcaaagttg ctcttatttc ttttaggaag aacaaaagaa
    19621 aatagcgcta taaatgaaag ataagtagcc gctacattaa actgttcttt tgaaaaagtt
    19681 acagtcatcg gaatgataat tgatattgag gaagtaatct taattaaaaa tatagcaata
    19741 ttttcaacat cgcgttggtt tttatttaac attgtccaac atgttttagc aacaataaaa
    19801 ataattacac atgatattag cacacagact gaaaaaagtt ttagaaatgc gatgtagtca
    19861 gtgttattga actccttcaa aatactattt ttattcaatg taataaaagg aattttttta
    19921 attgaagaca ggaaagataa ggtaataaac agaattatat atatggttaa agtcgcagta
    19981 agaatgtaat tgagcttttt cctcatagaa tcacctcact ttctaacgaa aattatacca
    20041 aaaaggagag aagaaataat gcaagaatta gtaattttga aaaataaaga agctgtaact
    20101 acgagcttac aagtggcaga aagtttcaaa aagaaacata agcatgtact agaagcaatt
    20161 gaatcaataa aaagatcggt cgaaaattcg gccaatgttg aagatgggtc caattttgga
    20221 cagatgtttg tggaagggaa cgagccagac tcatacggaa gaagtcggag agtttatttc
    20281 atgaatagag acggattttc cttgctagct atgggattca ctggaagtaa agcaataaat
    20341 ttcaaactaa aatttattga agctttcaac gaaatggaag atgttattcg gaagaatact
    20401 gttcctcaaa caattgaaga catgatgatc tatcaattag aagaaatgaa agatgttaaa
    20461 aaagatgttt ccatgcttaa agatactatg cgaattagcg gacaacaaga gtttgaaatt
    20521 aagcaaaaag gaaatatgaa agttatggaa gttctagggg gaaaagaaag ccgagcttat
    20581 gaagaaatca gcaaaaaagt attctcaaaa ttttggtctg aatttaaacg taccttttca
    20641 atcccaagat atggcgagtt acctcgtaag agattcgatg atgctgtttc atttattgaa
    20701 atgtggttac cagaaactgc gatccgcatg gaaatcgatc aactgaacag acaacaaaga
    20761 cttttcggtg atgaaaatga atagagctga agcgctaaga atagggacgg taattgctaa
    20821 tcgctggtgg agacacaata aaccaaacat cctaagccaa caacatatcg aaaagcaaaa
    20881 agcatggcaa caaataaaaa agtgactcag ccggcaagca tagagtcaca aaacatatct
    20941 aaggagaatt ttagcatatg aataaagaac tttccacttt agatcaatat ttgactgatt
    21001 ctgaatgggg caagtcgaat atcaaggaaa caaataatcg aaaaatcaga cgaaatcttt
    21061 tgacgaacga agaactatca tgtgatcaag atgacttggg aaattttgtg agtatttggg
    21121 atcatgttta ccttatccat ctatcaaaac attcaaataa acctgaatac atctatgtca
    21181 tcgaagatgg cttgattgat gcgctagaag agtacaacag agataacttg attgatatct
    21241 cttattacgg atcaggtaag aaatacattg ctgaaatgga ggcagaattt gatgagtgaa
    21301 atcaaaggga caacgaactt tgaaaaactt tttagtcgta agttaaataa aattctcaag
    21361 aaaaaaggaa attttgatta tttatcttgg gctcacgcgt gggagattat gaaaaagaat
    21421 gatccacagg caacggtaac tattaatgag tataaacact acagggttgt ttctggaact
    21481 catcaagact ttcttgttga ggaatataaa ccttttctta tggatgaaac agggacttat
    21541 gtatctgtct cagtaacggt taaaggacat acggaaacag a
//
LOCUS       DACDKM010000039        21421 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20834_2016
            SAMN12640370-rid9891663.denovo.039, whole genome shotgun sequence.
ACCESSION   DACDKM010000039 DACDKM010000000
VERSION     DACDKM010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640370
            Sequence Read Archive: SRR10040891
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 21421)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21421)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742835.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742835.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:04:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,012
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 29 of 75
            Pseudo Genes (incomplete)         :: 33 of 75
            Pseudo Genes (internal stop)      :: 19 of 75
            Pseudo Genes (multiple problems)  :: 5 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21421
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640370-rid9891663.denovo.039"
                     /isolate="E20834_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            102..803
                     /locus_tag="IV334_001366"
     CDS             102..803
                     /locus_tag="IV334_001366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HAP8943445.1"
                     /translation="MRVNKIDFHFSKLRYQPDFEHFSDEEFQQIKEHTVLRSYKKGQI
                     LFDDGDPRQRLYILTKGLVRLERYDESATYYYYDYVNTNNLFPMSGMFGEETYTYTAQ
                     AMTDIETFYLPVSYYEKLARKNPEQLIYMIQKISKVVEIHELRIQIGLTSSAFDRVKQ
                     NLFILKEELGVPTKDGHITIPYPITLKELAVNSGTTRETAGQVIKQLRDSGLLDYDKK
                     RFTFCKTATTPAMRE"
     gene            complement(1412..2428)
                     /gene="tsaD"
                     /locus_tag="IV334_001367"
     CDS             complement(1412..2428)
                     /gene="tsaD"
                     /locus_tag="IV334_001367"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HAP8943446.1"
                     /translation="MNEELILAIESSCDETSVAVVRNGTEILSNIVASQINSHKRFGG
                     VVPEVASRHHVEQITLCLEDALVEAGVSAEDLSAVAVTYGPGLVGSLLIGISAAKAFA
                     WAHQLPLIPVNHMAGHIYAARLVKPFQFPLMALLVSGGHTELVYMQEDGSYEIIGETR
                     DDAAGEAYDKVGRVLGLSYPSGKEIDQLAHQGKDNYHFPRAMIHEDNYDFSFSGLKSA
                     FINLVHNAQQRGEDLDKNDLAASFQASVIDVLITKTLRACQNYPVKQLIIAGGVAANQ
                     GLREGLQDALAAKLPEVELVIPPLRLCGDNAAMIGAAAHVEMQKRNFASYQLNADPSL
                     VLSE"
     gene            complement(2425..2889)
                     /gene="rimI"
                     /locus_tag="IV334_001368"
     CDS             complement(2425..2889)
                     /gene="rimI"
                     /locus_tag="IV334_001368"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HAP8943447.1"
                     /translation="MICRKKDFDEAYLAECVWMISENAYPHGSPWTKMQFLADIQQPH
                     SDYLLLAEDNQICGFIGYSKVIDEVEITNIAVAVSEQGKGHARHLLQLLITEQIKDSL
                     HVFLEVRLSNEAARKLYESEKFRILGKRKSYYRNPTEDAIIMSRKLKTETMK"
     gene            complement(2896..3438)
                     /gene="rimI"
                     /locus_tag="IV334_001369"
     CDS             complement(2896..3438)
                     /gene="rimI"
                     /locus_tag="IV334_001369"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HAP8943448.1"
                     /translation="MLKKFRAYARSFFQDKMIYTEKTVEQIDRRYVLRELTTEDIKEL
                     LDVEREVYNGELPWTKSAFISELKSPLPHLYLGIFDNGRMIGFIGSRAFGLDCHITNI
                     AVRPAYQRKRIGSLLIDEIENFAIMNRCETMSLEVRMSNQDAQRLYRKLGFQATTIKK
                     RYYTETKEDALDMIKLLENE"
     gene            complement(3422..4246)
                     /gene="tsaB"
                     /locus_tag="IV334_001370"
     CDS             complement(3422..4246)
                     /gene="tsaB"
                     /locus_tag="IV334_001370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HAP8943449.1"
                     /translation="MYYCTSFAFGIFILRIKKYATMEGENVIAERIKYMITLGIDTAN
                     QTLAIGVVEDEQILGQIQTNIKRNHSVTLMPAIDQLFADLQISPKDIDRIAVSDGPGS
                     YTGLRIGVTTAKTIAYTLDKELVGVSSLKTIAANCIGVDNIIVPMFDARRKNVYAGAY
                     RWKENRLETVLEDVHISASDLFTQLQKLNEAIYFVGSDCHKFFSEIKEILPSAMINTV
                     PAWDIPSGVTVAQLGALVEPVAEKQAFLPRYLKRVEAEEKWLETHTPGDESYVEKI"
     gene            complement(4335..5315)
                     /locus_tag="IV334_001371"
     CDS             complement(4335..5315)
                     /locus_tag="IV334_001371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HAP8943450.1"
                     /translation="MATIKDIAQLAGVSPATVSRVLNYDPDLSVGLATKQKIFEAAER
                     LNYTKHRKNAANTKQTLRLIQWYDDTEELEDLYYLSIRLGIEKKAEEANVTLLKETWD
                     SISDLPADGTIALGKFDEAQLLEMKGSQNALLLVDSDGTSQGIDSLVVDFKSSVQKVI
                     EHFVSHKAQHIAMLAGTEYTKKNHQRLKDPRIMAFQQVLAEKGYDEGPIIEADFSVES
                     GYQAVKQYLKTNPHVPDALFAANDALAIGALKAIQEKGLVVPEDISVMGFNDVSVAKY
                     VTPSLSTVKVYTEWMGELAVETILQLSNSKAPVSRKIIVETELIIRESTK"
     gene            complement(5339..6823)
                     /gene="galT"
                     /locus_tag="IV334_001372"
     CDS             complement(5339..6823)
                     /gene="galT"
                     /locus_tag="IV334_001372"
                     /EC_number="2.7.7.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose--hexose-1-phosphate
                     uridylyltransferase"
                     /protein_id="HAP8943451.1"
                     /translation="MSISQTICDFATLAIEAGGWMEMDRLYLQNRLLGMVGEDALESI
                     EVRPVKEESVDLLDQLVAKARENGVLSELPAEQEIFEAQLMDFLTPPPSVVNAFFAQH
                     YSKSPEEATEYFFNLSKKNDYIKTRAIAKNIKFPAESEYGELEITINLSKPEKDPKQI
                     AAEREAVKVDYPTCMLCMENEGYRGRLNYPARTNHRIIRMNLDGESWGFQYSPYAYYN
                     EHSIILSEQHRPMKITKETFARLLRITEVLPHYFVGSNADLPIVGGSILSHDHYQAGR
                     HEFPMAKAPIEKLIKLEDYPQVIAGVVKWPMSVIRLQSTEKNLLIEAANSVLEKWKAY
                     SDETVSIRAFSADGTPHHTITPIARRRDNYFELDLVLRDNNVSEEHPDGIFHPHKDVQ
                     HIKKENIGLIEVMGLAVLPPRLKPELVEVEHFLLGEENQMAEYHRPWAEQLKKEHPDL
                     TKDTVTDVVQKSVGQVFARVLEDAGVFKRDEKGQAAFERFTAIL"
     gene            complement(6835..7824)
                     /gene="galE"
                     /locus_tag="IV334_001373"
     CDS             complement(6835..7824)
                     /gene="galE"
                     /locus_tag="IV334_001373"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010767298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HAP8943452.1"
                     /translation="MTILVLGGAGYIGSHAVDQLVQKGYQVAVVDNLLTGHKQAVHPD
                     AHFYEGDIRDKEFLRSVFEKEPIEGVIHFAASSLVGESVEKPLMYFNNNVYGMQILLE
                     VMHEFNVNKIVFSSTAATYGEPKESPITEDTPANPKNPYGESKLMMEKMMKWCDQAYG
                     MRYVALRYFNVAGAKADASIGEDHTPETHLVPIILQVALGQRKALAVYGDDYDTPDGT
                     CIRDYVQVEDLIAAHILALEYLKEGNESNFFNLGSSKGYSVKEMLEAAREVTGKEIPA
                     EIAPRRAGDPSRLVASSEKAREILGWKPEYTDIKAIIKTAWDWHVSHPNGYEE"
     gene            8072..8239
                     /locus_tag="IV334_001374"
     CDS             8072..8239
                     /locus_tag="IV334_001374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3042 family protein"
                     /protein_id="HAP8943453.1"
                     /translation="MKKFVSGILVGSLATAAAVAGLVASVKKTVIDPIDEKEAMIEEN
                     RKKAMRKRVSR"
     gene            complement(8301..10112)
                     /locus_tag="IV334_001375"
     CDS             complement(8301..10112)
                     /locus_tag="IV334_001375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding protein"
                     /protein_id="HAP8943454.1"
                     /translation="MKIGIIGAGPRGLSVAERILHNNQDRHSLELFLFDPNGPGGRVW
                     RLNQPPELLMNSVSQQVTLFTDETLSSGGLVTQGPNLFQWSKGPATDYIEQTVTKNKQ
                     LIIEEINRLEKDQQSTRCLYGLYQRWFFSKLQEEFPASIQFVYSLVKKVSKRKDGYLL
                     QTDQQSYSIDQLVLASGHWENDETEEERVWVDYAKKNVLLYQLPSNPADVDLSKIPPK
                     ENVFLRGLGLSFFDYVGLFTVSRGGRFENEKGKLVYHPSGKEPIVYSGSRRGLPYYPR
                     GRNQKQGGAMAWPRLLTKENLEQWHQSGFLTGETFFDYLKKDVELFYYKKIIEEYHLP
                     IPKKNFEHDFLSLSSEECLGKHPALIPYRWDWSYLETPLAYQNEPFSEASRIFIEGQI
                     KEAEKGNCTGAFASTFDALKDWRDPIRQAIEWHVFSAKEYKEILWGWFTHLNSFLTIG
                     PPIIRTKELAALIDAGIFHLIEPPFDIQQKHNRYVISLQDKEISSRYLIEARLPQTSF
                     SRSSNPVIKNLKENKLVRPFSFSDNTGSYHSEAIDVERGTNRVIDASGEIQSDFYCYG
                     IPVEGVDWLTAAVARPYTDPWNLRQADKIAQLILTDK"
     gene            complement(10109..10474)
                     /locus_tag="IV334_001376"
     CDS             complement(10109..10474)
                     /locus_tag="IV334_001376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 family protein"
                     /protein_id="HAP8943455.1"
                     /translation="MIKLKRVYLDAEKEDGYRILVDRFWPRGISKEKAQLDLWLKEIA
                     PSNEARKTFDHVPERFASFAKKYQQELIFEEEKKQAIFKLSQLMKEHPVITFVYGAKN
                     EHQNHAVLLKKFIEMEACK"
     gene            complement(10637..11032)
                     /locus_tag="IV334_001377"
     CDS             complement(10637..11032)
                     /locus_tag="IV334_001377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HAP8943456.1"
                     /translation="MVLWVINIILLLIVLAIILWEVYLRVMAKRSATTLTEEEFREGM
                     RKAQVIDVREKDVFDAGHILGARNIPYTVLKDSLGSIRKDQPVYIYDQKKSLSIRTAN
                     KLRKAGYQEIYLLKGGYDGWSGKIKSKKV"
     gene            complement(11050..12012)
                     /locus_tag="IV334_001378"
     CDS             complement(11050..12012)
                     /locus_tag="IV334_001378"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family glucokinase"
                     /protein_id="HAP8943457.1"
                     /translation="MDKKIIGIDLGGTTAKFAILTPEGEIQQKWSIDTNILDDGKHIV
                     PEIIESINHRLNLYNMKAEDFIGIGMGTPGSVDSEAGTVIGAYNLNWTEVQFVKKLIE
                     AGTGIKFAIDNDANVAALGERWKGAGENDPDVVFVTLGTGVGGGIVAGGNLIHGVSGA
                     GGEIGHITVDPEGFECTCGKRGCLETVSSATGVVRLGRFLAEEYAGDSKLKAMLDNGE
                     EVTSKDIFEMAQEDDPFALMVVDRVCFYLGLACGNLGNTLNPSSIVLGGGVSAAGEFL
                     RSRVEKYFKEFTFPQVRESTKIKLAELGNEAGVIGAASLALKFA"
     gene            complement(12012..12224)
                     /locus_tag="IV334_001379"
     CDS             complement(12012..12224)
                     /locus_tag="IV334_001379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgQ family protein"
                     /protein_id="HAP8943458.1"
                     /translation="MESLYDVQQLFKKFGIFIYVGARIYDIELMTIELRNLYDSRLID
                     HDTYMTAWRILKREHRIEESRKKGNL"
     gene            complement(12245..12943)
                     /locus_tag="IV334_001380"
     CDS             complement(12245..12943)
                     /locus_tag="IV334_001380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HAP8943459.1"
                     /translation="MNYQQQIKLRAWLRRPLWTYAFLGIQTAVFLMMELFPRLEIPYY
                     AGMYGPYLVHFNEWWRLVTPIFIHFGLMHFVMNSLILYFMGQQIEAIYGHWRFFLIYM
                     FSGIMGNTASFAFNEANVLSGGASTSIFGLFGALFILGFHFKHNPAIQQLVRHFLLFI
                     VLTFVFGLLDTSVDVWGHVGGLIGGLVLGNVLGLPKQQTSYSIHQRILSTLVFVFLFV
                     ICILLGLKKYGLLV"
     gene            complement(12963..13505)
                     /locus_tag="IV334_001381"
     CDS             complement(12963..13505)
                     /locus_tag="IV334_001381"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HAP8943460.1"
                     /translation="MDQLKVRLRRKGIEKLKNLAKSGKKKEKEQRIYKQLFASEYWKS
                     AKVIGTTLSNEFELDTQPVIQRAEKEQKKIVVPKTLPKRQMAFYEMDEQSVLVRSSFG
                     VLEPQSEKLYLPEMIDLLIVPGIIYNRAGYRIGFGGGYYDRYLNNFHKKTCSLVFREQ
                     LNEQWQPESFDQKIQQLFID"
     gene            complement(13637..14641)
                     /locus_tag="IV334_001382"
     CDS             complement(13637..14641)
                     /locus_tag="IV334_001382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HAP8943461.1"
                     /translation="MKKIYWTFYFLLFVLVILSIGSLMIGTPFIYLDQLFATFIGKES
                     SSLALIVREFRVPRLLISLLAGACLGVSGYILQGVTRNELADSSILGINAGAGFLVML
                     YLGFFSHYSLPFLLLVVAFLGGVLAACCVYLAAYDRNGFLGMNRILLSGIAVNAGLSA
                     LTLLCTIQLSKENYGFVNAWLAGSIWGASWPYVWALLPWAVILIPLGGLYAQRIVLLS
                     FGQERAQGLGLNVAKSQKILLLLAVALASGSVAVTGSLSFVGLLAPHAAKLVVRKENN
                     WTFVLSGLFGSIFLLSADMVARVILPSGEVPTGILIALFGAPYFLYALFIRQKHALNS
                     "
     gene            complement(14638..15627)
                     /locus_tag="IV334_001383"
     CDS             complement(14638..15627)
                     /locus_tag="IV334_001383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HAP8943462.1"
                     /translation="MRTMKKNRLLLLVCFVLLIIFFVALSNGAATVEKADFWQALFDF
                     DRSKQSHQIIRNIRLPRVAGAFLVGSCFALSGALMQGVTRNPLADSGLLGINAGASLA
                     MALSFAFWPKASDFSVFLMSLAGSFAVTLLVFGFLQFSKMGMNPLQLILAGVAISSFF
                     TAISQALTQFFNLQQDLAFWFVGGAANITWKQLAVLFPLYILAVLVSFSLGKAISLIH
                     LSETHALALGSHPKRTRLIVFCLVAVLAAIAVSLVGPVSFIGLLVPPVVRGMVGTDYR
                     YVLPVSFLVGGGLVMLADFTARMINPPFETPFGLVIALIGVPFLLIQVRREQR"
     gene            complement(15624..16430)
                     /locus_tag="IV334_001384"
     CDS             complement(15624..16430)
                     /locus_tag="IV334_001384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP8943463.1"
                     /translation="MTRLRAADIAAGYENKKIIENMSVTIPEGKITSLIGPNGSGKST
                     LLKSFTRILPVKSGKIIVNNSDISTYSSKELAKKIALLAQTSEHPTGMTVEEVVSYGR
                     YPYQKLFSGMDTQDHEAIEWALDATGLAGFRMRDIATLSGGQTQRVWIAMALAQETDI
                     LILDEPTTFLDPAHQLEILQLLTEINQMKQTTILMSIHDINHASRFSDYLLAMKNGKL
                     ITEGTPEEVITKECMKKIFEIDATIVTLPRTNKPVFLTYDLLPKEQEDQL"
     gene            16596..17552
                     /locus_tag="IV334_001385"
     CDS             16596..17552
                     /locus_tag="IV334_001385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAP8943464.1"
                     /translation="MKLKTKVFTLSSILLLAGVLSACSPSSDKETATTANSTTSSNST
                     HTVTDTLGHKVEIPNAPKRIIGSYLEDYLIALGEKPIAQWTVGSGSIQDYLQDDLKDV
                     PTISYDLPYEKVLSFEPDLLLISSSATVEGGKYEQYSKIAPTYVVKNGTDVTWEEQLK
                     DVGKALAKEKEAEKIISDYQKSIKKTREELADKIENKTAAVLWVTNNSAFMVSKNRSS
                     GRIVYDDLEFGVPDLVEEVSKKATSDWSAVSSEKLAELDADYIILVNSDKDAAMFNEP
                     SWKNLKAVTDNHVLQFGPESSWLYNGPIASQEMVNDIKEKLN"
     gene            complement(17629..18147)
                     /locus_tag="IV334_001386"
     CDS             complement(17629..18147)
                     /locus_tag="IV334_001386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HAP8943465.1"
                     /translation="MFISMWAQDKNGLIGKNGLLPWRLPNDMRFFREHTMDRILVMGR
                     KTYEGMGDLSLPYRHIIVLTTQPNFRTKENAEVMHSIDELLSYSQTISEDIYVSGGSR
                     IFQELLPYTGRIWRTLIDSTFEGDTYIGNIDFSDFALIEEYEGITDQENLYAHRFQKW
                     ERVKNRVASKEE"
     gene            complement(18235..18384)
                     /gene="rpmG"
                     /locus_tag="IV334_001387"
     CDS             complement(18235..18384)
                     /gene="rpmG"
                     /locus_tag="IV334_001387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HAP8943466.1"
                     /translation="MRVNITLECTSCKERNYLTSKNKRNNPDRLEKQKYCPRERKVTL
                     HRETK"
     gene            18612..19058
                     /locus_tag="IV334_001388"
     CDS             18612..19058
                     /locus_tag="IV334_001388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2188 domain-containing protein"
                     /protein_id="HAP8943467.1"
                     /translation="MPWNMKDFPASMKNLDKLTRKKAIDIANALLDEGYPDNRAIPIA
                     IDQAKKWYEDASESEKRTFEKEKNPSKTDEHDTNPRAGKLLDSDVIVEYEEEQWIVKS
                     KGAKKASNHFDTKKEAIEKGKQVAQNKESTLFIYKKDGTKEKEIDY"
     gene            complement(19253..21148)
                     /gene="asnB"
                     /locus_tag="IV334_001389"
     CDS             complement(19253..21148)
                     /gene="asnB"
                     /locus_tag="IV334_001389"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HAP8943468.1"
                     /translation="MCGIVGFVNDKDNKKTIINTMMDRIVHRGPNSSGEYIDKHVALG
                     FRRLSIIDLEGGTQPIYNEDRTKIIIFNGEIYNYQPLREELIAAGHVFQTHADTEVLL
                     HGYEEWGTELLQKIRGMFAFAIWDNEKNELFGARDHFGIKPYYYAEMNGTFMFGSEIK
                     SFLPHPDFNKELNKEALKPYMTFQYSPLNGETFFKDVYRLPEGHYYTYKDGKIDIQQY
                     WDADFETKETHSRQEWIEKIDETVQASIEAHTVSDVEVGSFLSSGVDSSYVTSVLKPD
                     HSFSIGFDDKTYNEAIEARKLTELLDLDNTAAVIDGDMSFKAFPLIQYHLDEPDSNPS
                     CVPLYFLANLASQSVRVVQSGEGADELFAGYQTYGFHTNSKFIRVIAQGLKKLPKGTR
                     YNLGRKIGKMKNFHGRIHLYESLAPAKEYFIGHARVFEESEASEVLTPKYQTAPSVDE
                     IMTVHYEKTEGIKDEVNKMQYVDLHQWMPKDILLKADKLSMASSLEVRVPLLDIEVMK
                     LAQQIPSKFLLNQNNTKDIFRQAANKHLPEEWSNRVKLGFPVPIKAWLKEEHGYEQVK
                     ALFEADFAKEFFDQEKIMKLLDDHHEGRKEEQRKIWTIFSFLTWYKVYFVDETIPQAE
                     AIDYVTV"
BASE COUNT         6442 a         4549 c         3698 g         6732 t
ORIGIN      
        1 aagtattaaa acttgtttct aatgttggta tgttaaaact ttacaaattc tttaaaaaaa
       61 gtgacaataa atatagtgaa caataaggag aggacttttt aatgcgtgtt aataaaatcg
      121 attttcactt ttcaaaactc agatatcaac ctgattttga acatttctcg gatgaagagt
      181 tccaacagat caaggaacat actgttcttc gttcttataa aaaaggacag atccttttcg
      241 acgatggtga tcccagacaa cggttgtata tcttgaccaa aggactcgtt cgtttagaac
      301 gttatgatga aagtgcaact tattactact atgactatgt gaatacaaat aacctttttc
      361 ctatgtcagg aatgtttgga gaagagacct acacttatac agctcaagcg atgactgata
      421 ttgaaacatt ctaccttcct gtttcctact atgaaaaatt agcacgaaaa aatcccgaac
      481 agttgatcta tatgatacaa aaaatttcta aagtagttga aatacatgaa ttgcgtatcc
      541 aaatcggatt gacctctagc gcctttgacc gggtaaaaca aaatttattc attttgaaag
      601 aagaattggg cgtaccaaca aaagatggtc atatcaccat tccttatccg atcaccttaa
      661 aagaattagc agtgaatagc ggcacgacac gtgaaactgc tggacaagta atcaaacaac
      721 tacgtgattc cgggctgtta gattatgaca aaaagcgttt tactttctgt aagacagcta
      781 ctactccagc tatgagagaa taacgcgaac aaaaataagc agtgaccctg ataaatatca
      841 gggtcactgc ttattttcct ccctaaaact tctgaaagat ccggcagttt tagatcactg
      901 gacaagactc aaagattttc tatgtgtgtt tcagttatgt aatattcact gtttgcaaaa
      961 aatactgtta gattaactag atgaagcgtc ttgtcctcat ccgatctaga ttattcgata
     1021 ttcctcgaca ataaacgatt actcagtgag ggcaatcaga ggaacggata atgataaata
     1081 agctgtaaaa aatagcaaaa taacaataat gattaaaagt atgtaaaaaa tagggctttg
     1141 acaaaaacgt gtcacagccc tatttcgatt aaacggtgtg tacctgtcaa ctcttcggtc
     1201 acaagtcata atgttcataa acatgaggag ctgttttttg aaattgttcc ttgtgaatca
     1261 gagttaaacg acaggcttac gaactcttga ttaaacggcg ttcaaaagct tgcatacgcg
     1321 gtaataagaa cagccaggtc aaaaacatga gaagccgttt ttcctgggct gttcttatta
     1381 cttgatgcag ggcagctttt tcacaacctc tttattcaga taaaaccaag cttggatcag
     1441 cattcaactg atagctagca aaatttcttt tttgcatttc cacatgtgct gctgctccaa
     1501 tcattgcagc attatctcca caaagtcgaa gagggggaat cactagttct acctcaggga
     1561 gtttagctgc caaagcatcc tgcaatcctt cacgcaaccc ttgattggcc gctacacctc
     1621 cagctataat cagttgcttg actggataat tttgacaagc tcgaagtgtt ttagtaatca
     1681 atacatcgat cacacttgct tgaaagctag ctgctaaatc atttttgtct agatcttctc
     1741 cacgctgttg tgcattatgc accaaattga taaatgcact ttttaatccg ctaaaactga
     1801 aatcataatt atcttcatgg atcatcgctc gcggaaaatg gtaattatct tttccctgat
     1861 gagctagctg atcgatttct tttccgcttg ggtaagaaag acctaatact cttccaactt
     1921 tgtcataagc ttctcctgct gcgtcatctc ttgtctcccc aatgatttca tatgatccat
     1981 cttcttgcat atagactaat tctgtatgtc ctccgctaac aagaagtgcc atcaatggaa
     2041 actggaatgg tttaaccaga cgggctgcat agatatgccc tgccatatgg tttacaggaa
     2101 taagcggcaa ttggtgtgcc caagcaaaag ccttagctgc agaaatacca atcagcaacg
     2161 agccgacaag tcctggtcca taagtgacag cgactgccga taaatcttct gccgacacac
     2221 cagcttctac cagtgcatct tccaagcata aggtgatttg ttctacgtga tggcggctag
     2281 ctacttcagg tactacgcca ccaaaacgtt tatgactgtt tatttgagaa gcaacgatgt
     2341 tagataagat ctctgttcca tttctaacga ccgctacact cgtttcatca caacttgatt
     2401 caatcgccag aatcaattct tcattcattt catcgtctcc gtttttaact ttctgctcat
     2461 aatgatcgca tcttccgtcg gattgcgata atatgatttc cgtttaccta atatacgaaa
     2521 cttttctgat tcataaagtt ttcttgctgc ttcattagat agacgtactt ctaaaaaaac
     2581 atgcagcgaa tcttttattt gttccgtaat cagtaattgt aacaaatgcc gtgcatgccc
     2641 ttttccttgt tctgaaactg caacagcaat atttgtgatc tcgacttcat ctattacttt
     2701 cgagtatcca ataaagccac agatctggtt atcttcagct aaaagcagat aatcagagtg
     2761 aggctgttgg atgtctgcca aaaactgcat tttcgtccaa ggtgaaccat gtggataagc
     2821 attttcgcta atcatccata cgcattctgc caagtacgct tcatcaaaat ctttttttct
     2881 gcaaatcaac taatctcact cattttctaa aagttttatc atatccaacg catcttcttt
     2941 tgtttcagtg taatatcttt ttttgatcgt tgttgcttga aaccccaatt tccgataaag
     3001 tctttgtgca tcttgattac tcatacgcac ttctaatgac atcgtctcac agcgattcat
     3061 tatagcaaag ttctcgattt cgtcaataag cagcgaaccg attcgtttac gctgatatgc
     3121 cggtcgaacg gcaatattcg tgatatggca atctaaaccg aacgctcttg aaccgataaa
     3181 accaatcatt cttccattat caaagatacc tagatataga tgaggaagcg gtgacttcaa
     3241 ttctgatata aaggcacttt ttgtccatgg caattcgcca ttatagactt ctcgttcgac
     3301 atccagcagc tctttgatat cttctgtagt caattctctt agtacgtaac gacgatcgat
     3361 ttgttcgact gttttttctg tataaatcat tttatcttga aaaaagcttc tggcatacgc
     3421 tctaaatttt ttcaacataa ctttcgtctc caggtgtgtg cgtctccaac catttttctt
     3481 ctgcttctac tcgttttaaa tagcgaggaa gaaaagcttg tttctctgca actggttcaa
     3541 ctaaagcacc taattgagcc acagttacac cacttggaat atcccatgct ggaactgtat
     3601 taatcatcgc actaggcaat atttccttta tttcagagaa gaatttgtga caatcactcc
     3661 cgacgaaata aatagcttca ttcagctttt gtagttgtgt gaacaaatca gaagcagaaa
     3721 tatgtacatc ttcaagtaca gtttctaatc tattttcttt ccagcggtat gccccagcgt
     3781 atacattttt tcttcgagca tcaaacatgg gcacaataat attatccaca ccgatgcaat
     3841 tagccgcgat ggttttcaga ctagatactc ctactagctc tttatctaat gtataagcaa
     3901 tcgtttttgc tgtcgttaca cctatacgaa gccctgtata agaaccggga ccatcagaaa
     3961 ccgcgattcg gtcaatgtct tttggactta tttgaagatc tgcaaacaat tggtcgattg
     4021 caggcatcag tgttacactg tggttgcgtt tgatatttgt ttgtatttgt ccaagaattt
     4081 gttcgtcttc taccacaccg atcgccaaag tttggtttgc agtatctatt cctaatgtaa
     4141 tcatatattt gatcctttct gctatgacat tctcgccttc cattgtagca tactttttta
     4201 ttctgaggat gaaaatacca aaggcaaaac tagtacaata atacatgatt atctgctggc
     4261 gggcaaattt tgccccttcc tgaaacacaa aaaagctgcg gtatcactcc attttcccac
     4321 agcttttcca tcttttattt cgttgattct cgaataatca actctgtttc cacaataatc
     4381 tttcttgaaa ccggtgcttt ggaattactt aattgaagaa tcgtctccac tgctagctct
     4441 cccatccatt cggtataaac ttttacagta gaaagcgagg gcgttacata tttcgctaca
     4501 ctgacatcat tgaagcccat cacagaaata tcttctggta ccactaaacc tttttcttga
     4561 atcgctttca gtgcgccaat cgctaatgca tcattagctg caaaaagcgc atcaggaaca
     4621 tgcggattcg tttttaaata ttgcttcact gcttgatatc ctgactcgac agaaaaatct
     4681 gcttcgataa ttggaccttc gtcgtagcct ttttctgcca aaacttgttg aaatgccata
     4741 attctgggat cttttagccg ctgatggttt ttcttcgtat attctgttcc agcaagcata
     4801 gcgatatgtt gcgctttatg ggagacaaag tgttcaatga ctttttgaac acttgactta
     4861 aagtcgacaa ctagagagtc gattccttga gaagtgccgt cagaatctac caaaagcaag
     4921 gcattttggg atcctttcat ttctaataac tgagcttcgt cgaattttcc tagtgcgatc
     4981 gttccatctg ccggaagatc actaatggaa tcccacgttt ctttcaataa agtaacgttt
     5041 gcttcttctg cctttttttc gattcctagg cgaatagata aataataaag atcttcaagc
     5101 tcttccgtat cgtcgtacca ctggatcagg cgaagcgttt gcttagtatt tgctgcattt
     5161 ttacgatgtt tcgtataatt taagcgttct gctgcttcaa aaattttttg ctttgtggct
     5221 aagccaacgg aaagatcagg atcatagttg agaactcttg aaacagtagc aggagaaact
     5281 cctgccaact gcgcaatgtc tttaattgtt gccatttgtc tctcctcctt ttttacgttt
     5341 ataaaatcgc agtaaatcgt tcaaacgctg cctgtccttt ttcatctcgc ttgaatactc
     5401 cagcgtcttc cagtacacgt gcaaaaacct gacctacaga tttttgtacg acatcggtga
     5461 ccgtatcttt cgtcaaatca ggatgttctt tttttaattg ttctgcccac ggacgatgat
     5521 attcagccat ctggttttct tctcctaata agaaatgctc tacttctact agttctggtt
     5581 tcaaacgagg tggtaaaaca gcaagcccca ttacttcgat caacccaatg ttctcctttt
     5641 tgatatgttg gacatcttta tgtggatgaa aaatcccatc aggatgttct tctgaaacat
     5701 tgttgtctcg aaggacaagg tccaactcaa agtaattgtc ccttcttcgt gcaattggcg
     5761 taatcgtatg gtgtggtgta ccatctgctg aaaaagctct gatagatact gtttcatccg
     5821 aatatgcttt ccatttttcc aatactgagt tagctgcttc aataagcaag tttttctctg
     5881 ttgattgcaa acggatcaca gacatcggcc atttcacgac tcctgcgatg acttgcggat
     5941 aatcttctag tttgataagt ttttctatcg gtgcttttgc cataggaaat tcatgacgtc
     6001 ctgcctgata atgatcatga gaaagaatcg atcctccaac aattggtaag tcagcattcg
     6061 aaccaacaaa gtaatgaggc aagacttcag taatccgaag cagtctagca aatgtttcct
     6121 ttgtgatttt catcgggcgg tgttgttctg ataaaatgat cgaatgttca ttgtaataag
     6181 cataagggga atattggaat ccccagcttt caccgtctag attcattcga ataatccggt
     6241 gattggtgcg ggctggatag ttcaagcgac ctctatatcc ttcgttttcc atgcaaagca
     6301 tgcaggtagg atagtctact tttaccgcct cgcgttctgc tgcgatttgt tttggatctt
     6361 tttctggctt agataaattg attgtgattt ccagttcacc atactccgac tctgccggaa
     6421 atttgatgtt ttttgcgatg gctcttgttt tgatgtagtc atttttctta cttaaattaa
     6481 agaaatactc agttgcttcc tctgggcttt tcgaatagtg ctgtgcaaag aatgcattga
     6541 cgactgaagg tggcggtgtg agaaaatcca ttaattgcgc ttcaaaaatc tcttgttctg
     6601 ctggcaattc tgaaagaaca ccattttctc ttgccttagc gacaagctga tccaataagt
     6661 ctacagattc ttccttaact ggtcttactt caatgctttc tagagcatct tctcctacca
     6721 tccctaaaag acgattctgt aaatataagc ggtccatttc catccatccg ccagcttcga
     6781 tcgcaagtgt ggcaaaatca caaatcgttt gactgattga catatctcat tcccctattc
     6841 ttcataacca tttggatggc tgacatgcca atcccatgct gttttgataa tcgctttgat
     6901 atctgtgtat tcaggtttcc agcccagaat ctctctcgct ttttcacttg aagcgactaa
     6961 tcggctagga tctccagctc ttcttggtgc tatttctgct ggaatttctt ttcctgtcac
     7021 ttctctagct gcttctagca tttccttgac tgagtatcct ttgctgctgc ctaaattgaa
     7081 gaagttgctt tcatttcctt ctttcaagta ttccaaagct aaaatatgtg cagcaatcaa
     7141 atcttctact tgaacataat ctctgataca tgttccatct ggtgtatcat aatcgtctcc
     7201 ataaacagcc aaggcttttc tttgaccaag agctacttgt aaaatgattg ggacaagatg
     7261 cgtttctggt gtatgatctt ctccgataga tgcatctgct tttgctccgg ctacattgaa
     7321 gtagcgcaaa gcgacatagc gcatgccata tgcttggtca caccacttca tcattttttc
     7381 catcatcaat ttactttcac catacggatt tttaggatta gctggtgtat cttctgtgat
     7441 tggagactct ttcggctctc cataagttgc tgcagtagaa gagaatacga ttttattcac
     7501 gttgaactcg tgcatgactt caagtaagat ttgcataccg tatacgttgt tgttgaaata
     7561 catcaatggt ttttcgacag attctcctac taaagagctt gcagcaaaat ggattactcc
     7621 ttcaatcggt tccttttcaa atacggaacg tagaaattct ttatcccgga tgtctccttc
     7681 ataaaaatga gcatctggat gtactgcttg tttgtgacct gtgagcaaat tatctaccac
     7741 agctacttga tacccctttt gaactagctg gtctacagcg tgcgaaccga tataaccggc
     7801 tccgcctagt actaaaattg tcataaaaaa cactcctttg attagtaaaa ttgtgtttgt
     7861 tgattctata taatttaaaa aacgaagttt acttttatgg tgaaaacaaa caaagtaaaa
     7921 tttcgtaaat tcatatggta aatttataat aaaacattta gtaaacattt tcaagggttt
     7981 tttgcaaagg tttacaaaca aacgtaaaac tagccttttt ttctcttttg ttccataata
     8041 gatataacaa ataataaatg aggtggattc aatgaaaaaa tttgtatctg gaatattagt
     8101 aggaagtctg gcaacagccg ctgccgttgc tggtttggtt gcttctgtga aaaaaacagt
     8161 gatcgatcca attgacgaaa aagaagcaat gatcgaagaa aaccgtaaaa aagcaatgcg
     8221 taaacgcgtt tctcgttaat aattatttaa aaatacgcat gaagactttc taaaaaacag
     8281 ataagtcatc atgcgtattt ttatttgtca gtcaatataa gttgtgctat tttatctgcc
     8341 tgtctcagat tccatggatc tgtataaggt ctagctactg ctgcagttag ccaatccaca
     8401 ccttcaacag gtattccata gcaataaaag tcagactgga tttcccctga agcatcaatc
     8461 acgcgattcg tacctcgctc aacatctata gcttctgaat gataacttcc tgtattatct
     8521 gaaaatgaaa aagggcgaac aagtttgttt tccttcaaat tttttatgac aggattactt
     8581 gaacgagaaa agcttgtttg tggcaatcga gcttcaatca aatagcgact gctgatttct
     8641 ttatcttgta gagaaataac atagcggttg tgcttttgct gaatatcaaa tggcggttcg
     8701 atcaaatgaa aaatacccgc atctatcagt gctgctaatt ctttcgtccg aatgattggc
     8761 ggaccgatcg ttaaaaagct atttagatga gtaaaccatc cccatagtat ttctttgtat
     8821 tccttagcag aaaaaacgtg ccattcaata gcctgccgga ttggatcacg ccagtctttt
     8881 aatgcatcaa atgttgaagc aaatgctcct gtgcaattgc ctttttccgc ttcttttatc
     8941 tgcccttcaa taaatattcg gctagcttct gaaaagggct cattctgata ggcaagagga
     9001 gtttctaaat acgaccaatc ccatctataa gggattagtg ctggatgctt tccgagacac
     9061 tcctctgaag agagggagag aaaatcatgc tcaaaatttt tctttggaat cggcaagtga
     9121 tattcttcga tgattttctt gtaataaaac aactcaacgt cttttttcag atagtcaaaa
     9181 aatgtttcgc cagtcaaaaa tcccgactga tgccattgct ccaggttttc ttttgttagt
     9241 aaccgtggcc atgccattgc tccgccttgc ttttgatttc ttcctcgagg gtaataaggg
     9301 agcccccttc ttgatccact gtatacaatc ggttccttgc ctgatggatg atagaccagt
     9361 tttccctttt cgttctcgaa gcgccctcct cttgatactg taaacaatcc gacataatca
     9421 aaaaaactta agcctagacc tcttaaaaag acattttctt taggcggtat cttcgaaaga
     9481 tctacatctg caggattaga aggaagctga tacagtaaaa catttttctt tgcgtagtct
     9541 acccacactc tttcttcttc tgtctcatca ttttcccagt gaccgcttgc taaaacaagc
     9601 tgatcgatag aatagctttg ctgatctgtc tgtaaaagat aaccatcttt tcttttgctt
     9661 acttttttta ctaaagaata aacaaactga atagaagctg gaaattcttc ttgcaacttt
     9721 gagaaaaacc agcgttgata gagtccgtat aaacatcgag tagattgctg atctttttcc
     9781 agacgattga tttcttctat aattagttgc ttatttttgg ttaccgtctg ttcgatataa
     9841 tctgtagcag gtcctttact ccattgaaac aagttgggtc cctgtgttac caatcctcca
     9901 ctggacaatg tttcatctgt aaaaagcgtc acttgttgag aaacagaatt cattaataac
     9961 tccggtggct gattcaaccg ccaaactcga cctccaggac cattgggatc gaaaagaaaa
    10021 agttcaagag aatgtctgtc ctgattatta tggagaatcc tttctgctac acttaatcct
    10081 ctaggtccgg caccaatgat ccctattttc atttgcatgc ctccatttcg ataaattttt
    10141 tcaataaaac agcatggttc tgatgttcat tttttgcacc ataaacaaaa gtaatgactg
    10201 gatgttcttt cataagttga ctgagtttaa aaatagcttg cttcttttct tcttcgaaaa
    10261 tcaattcttg ctggtatttc tttgcaaatg aagcaaatcg ttctggtaca tggtcaaaag
    10321 ttttgcgtgc ttcattacta ggtgcaattt ctttcaacca cagatctaac tgcgcttttt
    10381 cttttgaaat tcctcgtggc cagaatctat ccacgagtat tctatagcca tcctcttttt
    10441 cagcatcaag gtaaacacgt tttaatttga tcatagttgt ttccttcttt ctaccttttc
    10501 gcttagctta tatatgtaga taatagcaca aaataaaaaa aatgaacggc acaactatcc
    10561 attgcgccgt tcatgtgatc ttggtcaaaa tctaatgatt gattcggttt ttaaagctgt
    10621 catacaatca tctgttttat acttttttac ttttgatttt tccagaccat ccgtcatatc
    10681 cacccttaag taaataaatc tcttgataac ctgctttacg taatttattt gctgtacgga
    10741 tgctcaaact tttcttttga tcgtaaatat agacaggctg gtcttttcga atagatccta
    10801 atgaatcttt caaaacagtg taagggatgt ttcttgcacc taaaatatgc ccagcatcaa
    10861 acacatcttt ttcgcgaaca tcgattactt gtgcttttct cattccttct cgaaattctt
    10921 cttctgtcaa agtcgttgct gaacgtttgg ccattactcg tagatagact tcccaaagga
    10981 tgattgcaag cacaatcaat agcaaaatga tattaattac ccataaaacc atgtaatgcc
    11041 tccgtttttt taagcaaatt tcaaggctaa tgaggcagca ccaatgacac cagcttcatt
    11101 accaagctct gctaatttga tttttgttga ttctcttact tgcgggaaag taaattcttt
    11161 aaaatatttc tctacacggc tacgtaaaaa ttctcctgct gccgatactc ctccacctaa
    11221 cacgatactt gacggattta atgtattccc caagtttccg caagctaaac ctaagtagaa
    11281 gcatacgcga tcaacaacca tcaatgcaaa cggatcgtct tcttgagcca tttcaaagat
    11341 atctttactt gttacttctt ctccgttgtc tagcattgct ttcagttttg aatcccctgc
    11401 gtattcttct gccaagaaac gacctaggcg taccacacct gtagcgctag atacagtttc
    11461 taaacatccg cgtttaccgc aagtacattc gaacccttca ggatccacag tgatatgtcc
    11521 gatttctcct ccagctccgc tcacgccgtg aatcaaattt ccgccagcaa cgatacctcc
    11581 gccaacacct gttcctagag taacgaatac aacgtctgga tcgttttctc cagcaccttt
    11641 ccatcgttca cctaaagcag caacattcgc gtcattatcg atagcgaatt tgattcctgt
    11701 tcctgcctcg atcagttttt tcacaaattg aacttctgtc cagtttaagt tatacgcacc
    11761 aataaccgtt ccagcttcgc tgtccacgct tcctggggta cccatgccga ttccgataaa
    11821 gtcttcggct ttcatattat ataaattcaa acgatgattg attgattcaa tgatttcagg
    11881 aacgatatgc ttaccatcat ccaaaatatt ggtatcgatg ctccattttt gctgaatctc
    11941 tccttctggt gttaaaattg caaattttgc tgttgttcca cctaaatcaa tcccaataat
    12001 cttcttgtcc attacaaatt tcctttcttt cggctttctt caattcggtg ctctcttttt
    12061 aatatgcgcc acgctgtcat gtatgtatca tgatcaatca aacggctatc atacagatta
    12121 cgcaattcga tagtcattaa ttcaatgtca tagatgcgtg cacccacata gataaagatg
    12181 ccaaattttt tgaacagctg ctgtacatca tataagctct ccatctttaa gcacttcctt
    12241 catattatac aagtaaacca tattttttta aacccagtaa aatacatatt acaaaaagaa
    12301 atacaaaaac tagtgtagaa agaatccttt ggtgtatcga atagcttgtc tgttgtttcg
    12361 gtagacccaa aacattccct aacactaatc cgccaattag cccaccgaca tgtccccaaa
    12421 catctacaga ggtatccaga agaccaaaaa caaatgtcaa cacaataaat aaaagaaaat
    12481 gtctgactag ctgctggatc gctgggttat gtttaaaatg aaaccctaag ataaacaacg
    12541 caccgaacaa accgaatatc gaagtacttg caccaccaga cagaacattc gcttcgttaa
    12601 aagcaaagct tgctgtattt cccattatcc cgctgaacat ataaatcaag aaaaaacgcc
    12661 aatggccata gattgcctcg atttgctgcc ccataaaata cagtatcagt gagttcatta
    12721 cgaaatgcat cagtccaaaa tggatgaaga ttggtgtcac taaacgccac cattcattga
    12781 agtgaacaag ataaggacca tacattccgg cataataagg gatctcaagt cgcggaaata
    12841 attccatcat gaggaagacc gctgtctgta tccctaaaaa agcataggtc cataacggtc
    12901 gtcttaacca tgcacgcaat tttatttgct gttgataatt catgaaaacc tcccatttca
    12961 ttttagtcaa taaacaattg ttggatcttc tgatcaaagc tctccggttg ccattgttca
    13021 ttcaattgtt cacgaaaaac aaggctacat gtttttttat gaaaattatt taaataccgg
    13081 tcgtaataac caccgccaaa accgattcga taaccggctc tattgtagat aatacctggt
    13141 acaatgagga gatcgatcat ctccggcaaa tagagcttct ctgattgtgg ctcaagaaca
    13201 ccaaaactcg aacgtacaag aacggactgt tcatccattt cataaaacgc catctgacgt
    13261 ttcggcaaag ttttaggtac aactattttc ttttgttctt tttctgctcg ttggataacc
    13321 ggctgtgtat ccagttcaaa ttcattcgat aaagtcgtcc ctatcacttt tgctgacttc
    13381 cagtactcgc tggcgaatag ctgtttatag attcgctgtt ctttttcctt tttcttgcca
    13441 cttttggcca gatttttcag cttttcgatt cctttccggc gtaatcttac tttcaattgg
    13501 tccaaaaact cacccttcct tctctcataa aatggtatag gaaaaaaaga aaaatagcaa
    13561 tcgaactttc aaataaatgc ataaatagaa taaaaagaag acctagaaat gattggcctt
    13621 ccttttttaa tgaatttcaa ctgttcagcg cgtgtttttg tctgataaaa agtgcataca
    13681 gaaaatatgg cgcaccaaaa agggcaatca aaattcctgt aggtacttcc ccacttggta
    13741 gaatcactct agcaaccata tctgcactaa gcagaaaaat ggaaccgaac aaaccgctca
    13801 gaacaaaggt ccagttattt tctttacgta cgactagctt cgcagcatga ggagctagta
    13861 gtcctacaaa agataaacta cctgtcaccg ctacactacc gcttgccaaa gcaacagcta
    13921 acaacaaaag gattttttga cttttagcca cgttaaggcc taatccttgc gcgcgttctt
    13981 gaccaaatga tagcaataca atcctctgtg cataaagtcc tcctagcgga attaagataa
    14041 ccgcccatgg aagcaatgcc caaacataag gccagctcgc accccagatt gatcccgcta
    14101 accatgcatt cacaaatcca taattttcct tagataattg gatcgtgcat agcaatgtta
    14161 atgcagaaag gccggcatta acggcaatac cggacaataa gattcgattc atacccaaaa
    14221 agccatttcg atcataggcc gcaagataga cacagcaagc agctaaaaca ccacctagaa
    14281 aggctacaac tagcaataga aacggcagcg aatagtgcga aaagaaacct aaataaagca
    14341 tcactaaaaa tcctgctccg gcattaatgc ccaatatact agaatctgcc aattcatttc
    14401 ttgttactcc ttgcaagata taaccagaaa cgccaagaca agctcctgct aataaactga
    14461 tcagtaatcg aggaactcga aattctctaa caatcagcgc caaagaagat gattctttcc
    14521 ctatgaacgt ggcaaaaagc tgatccaggt aaataaaagg cgtaccaatc attaaactac
    14581 ctatagacag gatgacaagt acaaaaagca agaaatagaa cgtccaatat atttttttca
    14641 ccgttgttcc ctccttactt gtatcagtaa aaacggaacg ccaatcaatg caatgactaa
    14701 tccaaaaggt gtttcaaaag ggggattgat catcctcgct gtaaaatccg ccaacataac
    14761 caatccaccg ccaactaaaa aagagacagg tagaacataa cgatagtccg ttccaaccat
    14821 tccgcgaaca actggcggaa ccaacagccc aataaatgaa acaggtccga ctaacgaaac
    14881 ggcaatggct gctaacactg caactagaca gaaaacaatc aaccgcgttc gtttaggatg
    14941 acttccaaga gctaatgcat gtgtttcact caaatgaatc aaagatattg cttttcctaa
    15001 tgaaaaagaa accaaaacag caagtatata taaaggaaac aatactgcta attgtttcca
    15061 tgtgatattg gcagctccgc ctacaaacca aaaagcaaga tcctgctgca gattaaagaa
    15121 ttgggtaagt gcttggctga tagcagtaaa aaaagaacta atagctacgc cagctaaaat
    15181 cagctgtaaa ggattcatgc ccattttcga aaactgcaaa aaaccaaata cgagcaaagt
    15241 tacagcaaaa gaacctgcca atgacattag aaacacagaa aaatcagaag ctttaggcca
    15301 aaaagcaaaa ctaagggcca ttgccaacga agcacccgcg ttgattccta gtaagcctga
    15361 atctgctaaa ggatttcttg tcaccccttg catcaatgca ccgctcaatg caaaacaact
    15421 ccctacaaga aatgctcccg ccacacgagg caaacgaata tttcgtatga tttggtgact
    15481 ttgttttgat ctatcaaaat caaacaacgc ctgccaaaaa tctgcttttt ccacagtcgc
    15541 tgcgccattt gaaagagcaa caaaaaaaat aatcaaaaga acaaaacaga ccaaaagcag
    15601 gagtctattt ttcttcatag tcctcataat tgatcctcct gctcttttgg caacaaatca
    15661 taggttagaa aaactggttt atttgttctt ggaagcgtga caatcgttgc atcaatctca
    15721 aatatttttt tcatacactc ttttgtgatc acttcttcag gtgtaccttc tgtgatcaac
    15781 ttaccgtttt tcattgctaa taaataatct gaaaaacgtg aagcatgatt aatgtcatga
    15841 atcgacatca agatcgttgt ttgtttcatt tgattgattt cagtcaataa ctgtaggatc
    15901 tctaattggt gtgccggatc taaaaatgtt gtcggttcat ccaagatcaa gatatctgtt
    15961 tcctgagcaa gcgccatagc gatccagaca cgctgggttt gtccaccaga cagcgttgcg
    16021 atatcccgca tacgaaatcc agctagacct gtagcatcca acgcccactc aattgcttca
    16081 tgatcttgcg tgtccatgcc actgaacagt ttttgataag ggtatcgtcc gtaagatact
    16141 acttcttcta ctgtcatacc tgtagggtgt tcactagttt gtgctaataa tgcaattttt
    16201 tttgcaagtt cttttgaact gtatgtagag atatcagagt tattgacaat gatctttcct
    16261 gatttcacag gtagtatacg ggtaaaactt ttcaataaag tagattttcc acttccattt
    16321 ggcccaatca aactagtgat tttcccttca ggaatcgtaa cagacatgtt ttcaatgatt
    16381 tttttgtttt cgtagccggc tgcaatgtca gctgctctta atctcgtcat ttcacccatc
    16441 ctttcgctga aaatcattct caattatgac gctgtttcaa catttcgtca agcgctactt
    16501 tcatatctat agccaaatga agcggttgac agcgttcgaa atatcaggaa taataaccaa
    16561 tgagaaagat tctcaattat gttaggagga aagaaatgaa attaaaaaca aaagtattca
    16621 cattatctag catattgctg ctggctggag tactgagcgc ttgctccccc tcttcagata
    16681 aagaaacagc tacaactgcc aattcgacga cttcatccaa tagtactcat acagtcacag
    16741 atacacttgg acataaagta gagattccaa acgcgcccaa acggatcatc ggcagttact
    16801 tagaagacta tctgattgca ctaggggaaa aacctatcgc ccagtggact gttggcagtg
    16861 gttccattca agattatctg caagatgatt taaaggatgt cccaacgatt tcttatgacc
    16921 ttccttatga gaaagtgtta agctttgagc ctgatttgct gttgatttct tcttccgcta
    16981 ctgtcgaagg cgggaaatac gaacaatata gcaaaatcgc gccaacttat gttgtaaaaa
    17041 acggaacaga tgtcacatgg gaagaacaac taaaagatgt cggcaaagct ttggcaaaag
    17101 aaaaagaggc agaaaaaatc atttccgatt atcaaaaatc catcaagaaa actcgagaag
    17161 aactagcaga taaaatcgag aacaagacag ctgcagtgtt gtgggtgacc aacaactcag
    17221 cctttatggt cagcaaaaat cggtcaagtg gaagaatcgt atacgacgat ctggaatttg
    17281 gtgttcctga tctagtggaa gaggtctcca aaaaagccac ttctgactgg tctgctgttt
    17341 cctctgaaaa gctagctgaa ttagatgctg actacatcat ccttgtgaac agtgacaaag
    17401 atgctgccat gttcaatgaa ccaagctgga agaatctaaa agcagtgaca gacaatcatg
    17461 tactgcaatt cggacctgaa tctagttggc tatataacgg acctattgcc agtcaggaaa
    17521 tggtcaatga tatcaaagaa aaactaaatt gattttaact aaaaaaagca tcttcacgat
    17581 tagtcgtgag atgctttttt tcttattaac aaactagacg catagatttt attcttcttt
    17641 tgaagcaacc ctatttttta ctctttccca tttttgaaaa cgatgggcat atagattttc
    17701 ttgatccgtt atcccctcat actcctctat aagggcgaag tctgaaaaat caatattccc
    17761 aatatacgta tcgccttcaa acgtgctatc gatcaatgtt cgccagatcc gaccagtgta
    17821 aggaagaagt tcttgaaaaa tccgactacc acccgaaaca tagatatctt ctgagatcgt
    17881 ttgactatac gacaaaagtt catcgatcga atgcattact tccgcatttt ctttcgtgcg
    17941 aaaatttggt tgcgttgtca acacgatgat atggcgataa ggaagtgaaa gatcccccat
    18001 tccttcatag gtctttcttc ccatcactaa aattcgatcc attgtatgtt cgcgaaagaa
    18061 acgcatatca tttggcaatc gccatggtaa caaaccattt ttgccgatca gaccattttt
    18121 atcttgtgcc cacatggaaa taaacatatt atccgctcct tatgatttta gatgttttga
    18181 gataaaaaaa gtctcaattc tttgaccagc aaggaattga gaactttaaa aacattattt
    18241 tgtttcacgg tgcaaagtaa ctttgcgttc gcgtgggcaa tatttttgtt tttccaaacg
    18301 atcaggattg ttacgtttgt ttttgcttgt tagatagtta cgttctttac aagaagtaca
    18361 ctctaaagtg atgtttacgc gcatgttttt ccctcccatt tttacaagaa tcctctaaga
    18421 tgactctcag acttgattat cttaccatga ttttttctag attgctagtg tttttcacga
    18481 ttttgttaag taaaactact tgacgataaa tacaacggtt ttacttctca aacaaaatct
    18541 ttttcttttt gtataaatac ccttacactt gtttatatag attatgtttt ttaataaagg
    18601 gggatactaa tatgccatgg aacatgaagg actttcctgc atcaatgaaa aacttagata
    18661 aattgacaag aaaaaaagca atcgacatcg caaatgcatt attagatgaa gggtaccctg
    18721 ataaccgtgc gatcccaatt gccattgatc aagcaaaaaa atggtatgaa gatgctagtg
    18781 aatcagaaaa acgaactttc gaaaaagaaa aaaatccaag caagacggat gaacacgata
    18841 cgaatcctcg tgctggaaaa ctgcttgatt cagatgtgat cgtagaatat gaggaagaac
    18901 aatggatcgt gaaatctaaa ggggcaaaaa aggcaagcaa ccattttgat acaaaaaaag
    18961 aagcaatcga aaaaggaaag caagtagcac aaaacaaaga atcaactttg ttcatttata
    19021 aaaaagatgg gacaaaagaa aaagaaatcg attattgaac atatgtaaag gggttgtgac
    19081 gaaaatcgtg tcacaacccc cttatgttcg aaacaatggt gttcctctgc caactccgaa
    19141 ccataagtca taatatctat aaaacgggaa gagctgtttt ttgaaattgt ttcttatcac
    19201 tcagtgttaa acggtaggag aaaacctctt ttttattgat atttgatttt catcaaacag
    19261 tcacatagtc gattgcttct gcttgcggaa tcgtttcatc tacaaagtag actttatacc
    19321 atgtcaagaa actgaagatt gtccagattt ttctttgctc ttctttacgt ccttcgtgat
    19381 ggtcatctag caacttcatg attttttctt gatcaaaaaa ttcttttgca aaatctgctt
    19441 cgaataatgc tttgacttgt tcgtagccgt gttcttcttt caaccaagct ttgattggca
    19501 cagggaatcc taatttcaca cgattcgacc attcttccgg aagatgtttg ttcgcagctt
    19561 gtctgaaaat atcttttgtg ttgttttgat tcaataaaaa cttacttggt atttgttgag
    19621 ccaatttcat gacttcgata tccaataaag gtactcggac ttccaaagaa cttgccatag
    19681 aaagtttatc tgcctttaac aagatatctt ttggcatcca ttgatgaaga tcgacatatt
    19741 gcatcttatt gacttcatct ttgattcctt ctgttttctc gtaatgaaca gtcatgattt
    19801 catccacaga tggtgctgtt tgatattttg gtgttaacac ttctgatgct tctgattctt
    19861 cgaacactcg agcatgaccg ataaaatatt cttttgcagg agcaagtgat tcatataaat
    19921 ggatccgtcc atggaagttt ttcatttttc cgattttacg tcctaaatta tagcgtgttc
    19981 cttttggtaa ttttttcaat ccttgagcaa tcacacggat gaattttgaa ttcgtatgga
    20041 atccatacgt ttggtatccc gcaaacagtt catccgcccc ttctccagat tggactaccc
    20101 gcacactttg tgaagcaaga tttgctaaaa agtacaaagg tacgcaagaa ggatttgaat
    20161 caggttcatc taagtgatac tggatcaaag ggaatgcttt gaacgacatg tctccgtcga
    20221 tcactgctgc tgtattatct aagtccaata gttcggttag ttttctggct tcaatcgctt
    20281 cattataggt tttatcatca aacccgattg agaaagaatg atctggtttc aatacagagg
    20341 ttacataact ggaatcaacc ccgctggata agaaagaacc aacttctaca tcactgaccg
    20401 tatgtgcttc aatagaagct tgaacggttt cgtcgatttt ttcgatccat tcttgtcggg
    20461 aatgcgtttc ttttgtttca aaatctgcat cccaatattg ctgaatgtct atttttccat
    20521 ctttatacgt ataataatgg ccttcaggca aacgatagac atctttgaag aatgtttcgc
    20581 cgtttaatgg agaatattgg aaagtcatat aaggtttcaa cgcttctttg ttcaattctt
    20641 tgttaaaatc aggatgaggc agaaaacttt tgatttctga accaaacata aatgttccgt
    20701 tcatttctgc ataataatat ggtttgatcc caaagtggtc tcttgcaccg aataactcat
    20761 tcttttcatt atcccaaata gcaaaagcaa acattccgcg gatcttttgc aataattctg
    20821 ttccccattc ttcgtaacca tgcaaaagga cttctgtatc tgcatgtgtt tgaaaaacgt
    20881 ggccagctgc aatcaattct tctctcaaag gctggtaatt atagatttcg ccattaaaaa
    20941 taatgatctt tgtccgatct tcattgtaaa taggctgagt accgccctct aagtcaataa
    21001 tacttaatct tctgaatcct aaagcaacat gtttatcgat atattctcct gaactattag
    21061 gtccacggtg aacgattcga tccatcattg tattaataat tgtttttttg ttgtccttgt
    21121 cgttaacaaa acctacaata ccgcacattt aactttcaca tcctaatata aaattactgt
    21181 ttatttcatt catatgcatc ttatcataga ataaatagcg gacgaatatt ttctctctta
    21241 caatttcgtc acgtgaaaac aaaagatgaa agtttctgtt tttacagaaa ataaagtccc
    21301 aatatctaat ttaccatgtt ttcttttttg aggctacctt attctcttcg tgttaaaaaa
    21361 atattagtgg atgctatttc caaaaaaacc agcaaaaaaa gacacgacct aaagatcgtg
    21421 t
//
LOCUS       DACDKM010000040        20789 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20834_2016
            SAMN12640370-rid9891663.denovo.040, whole genome shotgun sequence.
ACCESSION   DACDKM010000040 DACDKM010000000
VERSION     DACDKM010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640370
            Sequence Read Archive: SRR10040891
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20789)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20789)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742835.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742835.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:04:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,012
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 29 of 75
            Pseudo Genes (incomplete)         :: 33 of 75
            Pseudo Genes (internal stop)      :: 19 of 75
            Pseudo Genes (multiple problems)  :: 5 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20789
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640370-rid9891663.denovo.040"
                     /isolate="E20834_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..375
                     /locus_tag="IV334_001390"
     CDS             <1..375
                     /locus_tag="IV334_001390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8943469.1"
                     /translation="EYYDDYNDFDVKRYTKYIGTLRTQISPAEFQVILFNSLYIKRGF
                     GLGIQLIGSGFFGDDFDFETNQHFETSINEQWFLSLSTVDSDNSIKRQKLSEYIKELG
                     SVEYKKIANFESLYKLFNETKL"
     gene            419..1591
                     /locus_tag="IV334_001391"
     CDS             419..1591
                     /locus_tag="IV334_001391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HAP8943470.1"
                     /translation="MSLFDIFKQSIRNEEPSEWIPDFVYGEEVSARAYLKIMAKNTVL
                     DFVARTMSTLEVKFKNKDGTSDWDYILNVRPNSDMSAATFWQKFFYRLLDDNEVLVIF
                     TDDNQLLIADDFSRTEYAVYDDVFTNVMVKNFVFERSWNMSDVIYMEYNNEELERFTR
                     GLFEDYAALFGRILEVAMRNNQIRGSVSIDSTGTANDEKGKDGKTRSQRLQEYIDRIW
                     NSFKTNSVAIVPKVKGFEYEEYTNKQGVSNQSLDELNKMKSSLIDDVANAIGVPTALI
                     YGEKAELDSNIKAFRKLCIAPLVKKLQDELVAKIISRQEYKNGERIEVKKVLQKDLLE
                     YATQIDKITSGGAFYIDEVRDEVDYDELPNNEGKIRIRTKNYEETVKGGENDNAES"
     gene            1578..2282
                     /locus_tag="IV334_001392"
     CDS             1578..2282
                     /locus_tag="IV334_001392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010749273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Clp protease ClpP"
                     /protein_id="HAP8943471.1"
                     /translation="MPKAKKVPFQFTNEIQNGKHILTLSGNVQKKYWRDDDVINAKDI
                     RDSLDTVTDDIVIKLNSPGGDVFEGIEIYNYLKDHPSNITVEVTGLAASAATFIVAGA
                     NDVIMNVGTSLMIHEASTFAWGNKQDIQKTLNALETIDDSILAIYSDKTGQSVDKLRE
                     WMNEEKWFTADEAVKYGFANSVKREEPQNEPQNIAAMIQDAVAVAIANLKEPVTNKIE
                     QESKQKSLIARLRKGE"
     gene            2287..3447
                     /locus_tag="IV334_001393"
     CDS             2287..3447
                     /locus_tag="IV334_001393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HAP8943472.1"
                     /translation="MLKITDKTADAKKAFNAISSKEDATPEQINAALEEYVTAIAEDA
                     GKQVRAEYEELKNVTDNRVLEARGIPTLTAEETKFYNEAVKSGGFDSDVVWPETILER
                     VFENLQNDHPILGLINFTPTVGRVKVIRARRKGVAVFGPLHKDLEGQLDAEFGATEFV
                     QLALTAFFLISNDTLDLGPRWIDRFINLSLSEAVRDVWAKKIITGSGKDEPIGLLKDL
                     DGAVTSGVYPDKASAGTLTFAKEKIVLELAGVMKKLSKYTYKVDGDDEGEVKYRSVAG
                     RVYLIVNPVDYWDIVARVTFANLNNVYGTTMPFIAVDHIIESVDVPESKCIAYVDKEY
                     EATQSRAEKVYVYKETFAMKRATLYAVDMLGNGYPTNNDAAQVYDLDFTAGE"
     gene            3523..3801
                     /locus_tag="IV334_001394"
     CDS             3523..3801
                     /locus_tag="IV334_001394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage gp6-like head-tail connector protein"
                     /protein_id="HAP8943473.1"
                     /translation="MNTEEFIKEYKARFRIFHTSEDEDIGKQLESGFADIKSLIGSFD
                     PSTYEKGKELVYERTRYLRNESLEYFYDNFQTMIMDASIDLAGAGYAN"
     gene            3782..4153
                     /locus_tag="IV334_001395"
     CDS             3782..4153
                     /locus_tag="IV334_001395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002380685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head-tail adapter protein"
                     /protein_id="HAP8943474.1"
                     /translation="MLDMPIKSNYKKPKIVAGDLNTPVTFFEFQPNKGPEPGDEEKKE
                     LYFCTALVYNPSMKDRDILGTKEGVTIKIRDPHQSYIPTNKHKVRILDYRYGDKVWEI
                     IDVSPDLEENSIVKIVLELIT"
     gene            4169..4573
                     /locus_tag="IV334_001396"
     CDS             4169..4573
                     /locus_tag="IV334_001396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8943475.1"
                     /translation="MSNVKFEGVQETIQALEKKYGEKKTKTLTKKAINVGAEKVEKQL
                     QTDMTVFKDKGYTIAEVVRKNATYRDYKAEAEIGWNGPHQRYRIIHLNEWGYTRKGKQ
                     IRPRGFGVITKSLKNSELLYFDAVGEEVKKSL"
     gene            4570..4980
                     /locus_tag="IV334_001397"
     CDS             4570..4980
                     /locus_tag="IV334_001397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8943476.1"
                     /translation="MKDMLNIIYEALCLNEYIHSMTYNENTEQYRIKFYEQPETADKT
                     GPMITIRPVDTPNEAYHGSNKELSVEHMIQIDVESKYRATCKQIQYEIKKEMKKLGFG
                     QISGQGLDEYFTETNRFVDARRYEGNTKIYDTEY"
     gene            5041..5628
                     /locus_tag="IV334_001398"
     CDS             5041..5628
                     /locus_tag="IV334_001398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAP8943477.1"
                     /translation="MTLVGFKKMTIGVFDSTGKIPAANLYVIEGEQNKGATVSAEISG
                     LSKEPSKVYGSNIAYYVSQKGTGDVSATFGLLDLPTEVNDKILGYKTDTNKISFLGED
                     TEPPYCAILMESEDLNGDTAMLAMFKGKFSRESINLNTTTNEAFEPEAEEYVFSAIAN
                     DVEGDAKGQTVAKYVGDEEASITGLKEMVFPAAGE"
     gene            5701..6168
                     /locus_tag="IV334_001399"
     CDS             5701..6168
                     /locus_tag="IV334_001399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig domain-containing protein"
                     /protein_id="HAP8943478.1"
                     /translation="MPDTFKIYKKNGTEFTKVAEGESPLSITGIAANTQVAKGDYQAT
                     RVVGGQESVKVDIPAFTTLPISVTGVTLDKTTAEVEQGGTLKLTPTVTPANATDKTGS
                     WSSSNTAVATVTGGNVTVKTDAEVGGTTEVSFTTTDGGKVAKCTITVIEKAAG"
     gene            6214..6522
                     /locus_tag="IV334_001400"
     CDS             6214..6522
                     /locus_tag="IV334_001400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8943479.1"
                     /translation="MAKVRIELKEESGKKVYENIDTTGKDYRKALETIKKLNEDGVMI
                     WNRLDIYLDFAVGIFRDSKLTAEQILDGLPSEKVMSTLDDILGEVMGIESNPDPDAKK
                     "
     gene            6717..13451
                     /locus_tag="IV334_001401"
     CDS             6717..13451
                     /locus_tag="IV334_001401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HAP8943480.1"
                     /translation="MANGKPLGNMIIKLDLDSSAFSKGLAGAKNAVNHQMKAMKSQMQ
                     VMNSSGNSLGALQAKYNGLGGVLQANEKQVELLTKAYKDSFDSNGNATASTAKYANQL
                     NQATARSASYESQMKTTVGQIARMKVETEGFTGKLKANSDVLINSGKKIEAFGKGVSG
                     IGSALTVGVTAPIVAGATAVTSAAISWESAFAGVKKTNDEVVDSNGNVVYSYSDLEKG
                     LRNLATQLPGSHQEIANVAEAAGQLGIKTENVVSFTKTMIDMGESTNMSAETAATSLA
                     RFANITQMSQKDFDKLGSVIVDLGNNFATTESEITEMGLRLAGAGKQIGMSQGEILGF
                     ATALSSVGVEAEAGGSAFSKVMIQMQLAVEKGFGAFDQLKQMAEEQGVPWVNLVNAVR
                     DGGKSLKAVSEQMGFTSSDLKKMYKEADNSKSSLENFANVAGVTSEQFQKLFKSDPSE
                     AIIKFIQGLKDSEKHGTSAIKVLDDMDIKEVRLRDSLLRAANASDIFKGAIEKGNGAW
                     KQNTALTNEANKRYETTESKLKILKNEVIDTAIDLGGPFVDALRDGLNVSKPLIKNLG
                     NMAKAFSAMSDDQQRNIIKWVGLAAAAGPALKLLGTGTSVIGKVTSTTGRLTKSLVDL
                     AAKAAEKKAMDAFASSVTTAGAAAAKTAGAGGIGGLVPMMGKTASAASTAAGASGIGA
                     MTTSLGLLGPALLGIVGVGGALAVGYGAWKLFGEEAWNSSQRVKQWGTDVGEEIDSTL
                     DGVQKNIEGANGQFGLLKEGFTVDDANNMAANFATAGTSLETSLTGRIKALDDALKGL
                     PQSVQDAMKEVAESEEETLGQSLSKIKDNNEEIKKIRERAANQNRDLTVSELQQIQDL
                     MKGTSQAYVDTLDVSAKDRKKILSAMNGDVAQATEEEAKTWLQSLAKQKAATTENYRQ
                     MLEEKKAALKEKGVSKEFIDALEDEMNDYIKTTNQGFDSQIAAITNKYPQLINEVSLS
                     NGQLFSKTAAAMDNTGQYTKVFLESNEKILAQAKKTSDEIADNAEKNAEQLGMVADEA
                     THAGSTWNGLVLDEKTGKVKTNVQEVVNEATKDFTKWNELKPVVHDAKLTTNAKDVIS
                     IAAVQNGYWDDMEWKEKEALLEDECSKNVYKALENAGKWNELDIPSKEAILTSNTPEV
                     MGETLMNLGLWDTYKLQVKDLDLNAYKVYDAIDGSEEKLNSWAQLDNPTKELLLDNSD
                     FSTKLFNSEEMFKRWNQLSSNAKFLSANNTKFTEVLLSSDRMWNQWVNLPDNEKRLLA
                     DNEDLATTVLSSEEIYGRWTQLPDNQKYMLADNTDLANKIFASEESWNAWKAIPDTVK
                     NLLGNNIDLLAKVKDGTVSVEEYNKVMPLLKTLYGDNVNLKEAISGAKSSIENYNKNI
                     FPSDKTAVGHDEASQAAKDAFEAFNIFQTKIPDKITKTVSADFIGPMPKNAKGTNFHP
                     GGAAMVNDQKGPTYEELITLPSGETFIPKGRNVVLDLPRGSKVLNATKTKRLVPKYAD
                     GIGNITTVSSTLNLDALILAINELTTVLRTQLMAGTTDAKDTKLSDETKVADPIIPDS
                     LSEKSDQYLGIGAQWLTNLMNGWNSAVPQYMNSEMVFITNYLNALKLQNNPNYTQGAT
                     WNKNLLNGWNSLTGTFIATINSFCNQAMVTLRNYNTPMYNNGRTWQQNNLNGWNSLYG
                     SFIARVNQLGNDSINNLRSKSGGFYNAGTFLLQSLINGMNSLGNSLSTTMNNVANTMV
                     GGIGKGVNGVISGVNYVLKEVESSKNIGNWPIPQYAKGTDGHPGGLAMINDQKGLVHE
                     EYVQMPDGRSFIAKGRDLLVNLPKGAQVLNASLTKKLKERLNIPHYEKGIGNLDIVDL
                     LDDEKRMLEFLTSKVDFSGINEPWLDMTKSGTSLMSKAANTMLQSKLSEFFTHGNFDG
                     AVNANGVYQYLVDVAQKVMGKFPGLTVTSGYRAGDAYYHGKRQAIDLAYPGISGDPRY
                     TAAANYAFEKFPSKIAYVITNGRVRDRMGLSGTRSSGQWTNWPDGDHFDHIHLNGSMG
                     SGDIFNGGVGGSGVARWRSYVSKALKMNGLPATAAYINAWMSQIQTESGGNEKAIGGN
                     DGLAEGNATGLLQTKPGTFAANAFPGHGNIMNGFDNMLAAINYAKKRYGVAGMLQVIG
                     KGHGYANGGLITKDGLYRAGEGNKPEMVIPLTRKTRAIELMGQALAFLSGSNKKETKS
                     EINSVDNRHILQAIEKQSRMTNDLLSLLLSFFKSNNRSDKELALDIQKILIRRT"
     gene            13455..14327
                     /locus_tag="IV334_001402"
     CDS             13455..14327
                     /locus_tag="IV334_001402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8943481.1"
                     /translation="MRTVILRNKLNEEIDLSTEDYFATEMSNLGFEVTKEHIGQWGSF
                     REIGESIELSEFQSSVMISVHGFREQQLYGSLVEFLANGPFELEFSFDSETMIRKCSL
                     KSLSKTEIDSQSSLLTDTLNLYFTSSWYSIKREKLIQNSTSLNTRGKVYSFVRPYVYT
                     KNVWEKKGVFKFSNNSVYLTNSTERMSPLKIRIIGECSNPYWEVIQNSKVVASDGYFI
                     NMKDTQILEVSSLLEDQTAILKDVGGVESSVYQQQDFTKSNFVQAPLGEFSVVFHVGH
                     ANVEIELYEERDLF"
     gene            14324..14951
                     /locus_tag="IV334_001403"
                     /pseudo
     CDS             14324..14951
                     /locus_tag="IV334_001403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176402.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            14912..15373
                     /locus_tag="IV334_001404"
     CDS             14912..15373
                     /locus_tag="IV334_001404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8943482.1"
                     /translation="MQSPGPGNENKLLIVDKAMKDIENPLVLSTWYLDDENNLTQDST
                     NKNIALPTVNLVNIYDQTQEDRPSYEEVAKSELKGNAYSHEIKVNVVLNARNINVENI
                     ETGMLATIVYKEQTYKSVLTAWRISSSKKNIELTFGNVRSRFMDYFEDNGG"
     gene            15377..16201
                     /locus_tag="IV334_001405"
     CDS             15377..16201
                     /locus_tag="IV334_001405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8943483.1"
                     /translation="MVSNVDGYQFENVKVSAENDARLYHVLYNRKNQVIDGYDQSMNL
                     SSSGLTVKVAAGAAIIQGRMVVVRQEESITVPANSSGYIALTVDLTQEVIPGSILPES
                     EEYEWTNNQVKLEFITKVIKGNLNNGDKVYNLPLCSVNSTGSTVSISKISDSYELTLS
                     KGEILWRGTALMHDTQTVQPSKKIWQTVSGFLLVWLPYENGQAIEDRYVTTPFYKERI
                     TFTNVLGEIVSGFDDYHKKWFSKRINYNSNTNIFTGAASNASGDNANMVLRYIVSF"
     gene            16219..18261
                     /locus_tag="IV334_001406"
     CDS             16219..18261
                     /locus_tag="IV334_001406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BppU family phage baseplate upper protein"
                     /protein_id="HAP8943484.1"
                     /translation="MANLEIKLSANKRQPYLRHRVVGRVGDGGLTTINVQLLEEDEIT
                     PFVINLNGTLKFVGEVSNGNYTEGEPEIIDSTNGLISYTFTKSNFSTSHQFKQAYFEY
                     VDPNGKKVTFQNFIIDVLERVDINSEQAKYYISSLEKLQSEMQTTFNQFISDKQVQYD
                     QIYSKYNELVRLINESDKQVNDRIDKANNRIDQTNQQIGDLGKLKKMYSNSIDFGGYD
                     YSGKPNLMSKLKSSDFNVGYHGSLTSDNEKLHFTSDGTGSIIMFTRINTPQLASGKTY
                     TLSAKVRFDEGTTGAIDKLRLVYRTSPGEKILLEANSTNITTDDVGKEITIKGTANVN
                     YQITNLDRFYMSISFVDRDKINGGFKLYDIKIEEGSTATPYQPNLLDDPYWVGKTSLG
                     ENIANKGTTFPIKSSAYEIYKGNTEEELIIGQTYTITLKGTKPASQTFVAYNYWNVNF
                     GELKPVEGLTDVWSLTFTPTKLEPGLPKDLRIFQLPKETAGACQIDWLKIEKGDTRTP
                     NISQFKYFGEGLKDSNNPNDYSWDITPEYTEKGLNDTVSLTEPQSIEGLKNFEDGIQS
                     KGKSVLTSDDNKYEVVTLTVTNGNTGSAKLYREGKTVTIYFFALNGKSSGGNDSTILT
                     IPEGYRPPISFEQLVGSIDRSTLNSAQLSIGADGAIKWRRNSSYGSAYSFVITYSI"
     gene            18421..18867
                     /locus_tag="IV334_001407"
     CDS             18421..18867
                     /locus_tag="IV334_001407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8943485.1"
                     /translation="MKNIWKYGRTGGEYAGKVLDDMLVSVPYTDQPPLEGVRSDGEPL
                     TIADQMFDPKLNQWIILANALDHNDLNNLKAMYEALEHENGNLKQLNAKLMLSDVAIK
                     QENTALKEKADSLAQINSKMMLASLQNSKDISEIKEQLNPASKGGE"
     gene            18869..19006
                     /locus_tag="IV334_001408"
     CDS             18869..19006
                     /locus_tag="IV334_001408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XkdX family protein"
                     /protein_id="HAP8943486.1"
                     /translation="MFSFSDVKMMYDWGCFTDDQVRLFVPLCITDEEADKIINKDKSA
                     S"
     gene            19043..19336
                     /locus_tag="IV334_001409"
     CDS             19043..19336
                     /locus_tag="IV334_001409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8943487.1"
                     /translation="MINLGEWGMIAGSITAIVSLILLVIRPIAASFSKITETLSKVSH
                     NLDLLTKDLESSKSDRLMIHEELKKHDERLDTHTEKLVEHTQQIKTLFRERRK"
     gene            19333..19557
                     /locus_tag="IV334_001410"
     CDS             19333..19557
                     /locus_tag="IV334_001410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holin"
                     /protein_id="HAP8943488.1"
                     /translation="MNNKTFEVLKWFALVIIPALATFVGLVGKALNWQYTDICVVIIT
                     GFGAFLGSVLGVSNRTYKMFSAESEEGGNK"
     gene            19554..20573
                     /locus_tag="IV334_001411"
     CDS             19554..20573
                     /locus_tag="IV334_001411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HAP8943489.1"
                     /translation="MKKKITITAMSLLTALFLLPINGFAYTINNEFNLGANEGSSQVA
                     NNQYILLHETANETATGRNEAQYMQRSWTSAYTAYIVGDGGIVYQVGQPGYVQYGAGS
                     YANANSPVQIELQHTHDKATFEKNYKAYVELARDSAMKYGIPLTLDTPYNQPGIKSHL
                     WVTQNIWGDHTDPYGYLSEMGVSKEKLAYDLAHGFTDENPTTSENKPVIDPTRAGAAN
                     PTLSDGTNHSHIDQFGEIENANLHVAGWHIANYKYEYIFIMDYNTGKELARVRADGIY
                     RPDVNQAYNTSGNVGYHVSFNMRNFPNKKVYVMMRATNDPEGNTKGGAQDFHDKRWYL
                     NIPKR"
BASE COUNT         7400 a         3272 c         4403 g         5714 t
ORIGIN      
        1 gagtattatg acgattataa tgattttgat gtgaagaggt atacaaaata tatagggaca
       61 ttaagaacac aaatttctcc agctgaattc caagtgattt tattcaattc tctatatata
      121 aaacgaggtt ttggcttagg aattcaactt attggaagtg gtttttttgg agatgatttt
      181 gattttgaaa ctaatcaaca ttttgaaaca agtatcaatg aacagtggtt tctatcacta
      241 tcaactgttg acagtgataa ctctataaag cgacaaaaat tatctgaata tattaaagaa
      301 ttaggatccg tagaatataa aaaaatagct aattttgaaa gtttatataa actctttaat
      361 gaaactaagc tctagaaata gggctttttt atttttcttt gaaaggtggt gaaaacatgt
      421 gagcttattt gatatcttta agcagtcaat aagaaatgaa gagccttctg agtggatacc
      481 ggattttgta tatggagagg aagtatctgc tcgggcatac ctcaaaatta tggcaaaaaa
      541 tactgtttta gattttgttg caagaactat gtcaacgtta gaagttaagt ttaaaaataa
      601 agatgggaca tcagactggg attatattct aaatgttcga cctaattctg atatgtcagc
      661 agctacattt tggcaaaaat ttttttatcg gctcttagat gacaacgaag tgttagtaat
      721 atttacagat gataatcaat tgctgattgc tgatgatttt agtcgaacgg agtatgccgt
      781 gtatgatgat gtttttacaa atgtgatggt aaaaaatttt gtctttgaac gttcatggaa
      841 catgtctgac gtcatttata tggaatataa taacgaagaa ttagaaaggt ttacacgagg
      901 tttatttgaa gattacgccg cgttgttcgg acggattcta gaagttgcaa tgcgtaacaa
      961 ccaaattcgt gggtctgtat ccattgattc aacgggaacg gcgaatgatg aaaaaggaaa
     1021 agatggtaag actagatctc aaagattaca agaatatatt gataggattt ggaactcttt
     1081 taaaactaac tcagttgcaa ttgttcccaa agtgaaaggc tttgagtatg aggaatacac
     1141 caataaacaa ggcgtatcga atcaatcgct agatgaactg aataagatga agtcgtcttt
     1201 aattgatgat gttgccaacg ccataggagt acctacggcg cttatttatg gtgaaaaagc
     1261 tgaactggat tcaaatatta aagcttttcg taaattgtgt attgctccac tagttaaaaa
     1321 acttcaagac gaattggtgg caaaaataat tagtcgtcaa gagtacaaaa acggggaacg
     1381 tatagaagtt aaaaaagttc tccaaaaaga tctattagag tatgctactc aaattgataa
     1441 aataacatct ggtggtgcgt tttatattga cgaagttcgt gatgaagttg attatgatga
     1501 gttgccaaat aatgaaggaa aaattcgtat tcgtacgaaa aactatgaag aaactgtgaa
     1561 gggaggtgag aacgacaatg ccgaaagcta aaaaagtacc atttcagttt actaacgaaa
     1621 ttcagaatgg taagcacatt ctcaccttga gtggaaatgt acagaaaaaa tattggcgtg
     1681 atgatgatgt cataaacgct aaagatattc gtgactctct agatactgta accgatgata
     1741 ttgtaatcaa gttgaacagc ccaggcggtg atgtgtttga agggattgag atttacaatt
     1801 atttaaaaga tcacccatca aatattactg tagaagttac agggcttgcc gcaagcgcag
     1861 caacgtttat tgtcgctggt gccaatgatg tgattatgaa tgttgggact tctttgatga
     1921 ttcatgaagc ttctacattt gcttggggga ataaacagga catccaaaaa acgttaaacg
     1981 cattggaaac gatcgatgat tctatcttag ctatttattc agataagact ggccaatctg
     2041 ttgataagtt gcgtgaatgg atgaatgaag aaaaatggtt tacagcagat gaagctgtca
     2101 aatatggatt tgccaattct gtgaagcgtg aagaaccaca aaatgaacct caaaatattg
     2161 cagcgatgat tcaagatgct gttgccgttg caatagcgaa tttaaaggaa cctgttacta
     2221 ataaaatcga acaagaatca aaacaaaaat cattaatcgc acgattgcga aaaggagaat
     2281 aaagatatgt taaaaattac agataaaact gcagatgcaa aaaaggcatt taatgctatt
     2341 tcttcgaaag aagatgcaac acccgaacaa attaatgctg ctctagaaga atatgtaacc
     2401 gccattgcag aagatgccgg caaacaggtg cgtgctgaat atgaagaatt gaaaaatgtg
     2461 acggacaacc gtgtattaga ggctcgtgga attcctacac taacagcaga agaaacaaag
     2521 ttttataatg aagccgtaaa atctggaggt ttcgattctg acgtagtttg gccagaaacg
     2581 attttggaac gtgtttttga aaatcttcaa aacgatcatc ctattttagg cctcattaac
     2641 tttacaccaa ctgtaggtcg tgtcaaagta attagagcac gtcgtaaagg tgttgctgtt
     2701 tttggaccat tgcataaaga ccttgaaggc caactagatg ctgaatttgg tgcaactgaa
     2761 tttgttcaac ttgcgttgac cgcatttttc ttaatttcta atgatacctt agatttagga
     2821 ccacgatgga ttgaccgttt tatcaatctt tctttaagtg aagcagtacg tgatgtttgg
     2881 gctaaaaaaa ttattacagg ttctggtaaa gatgagccaa ttggattgct taaagatcta
     2941 gatggtgcgg tcacaagtgg tgtatatcca gataaagcta gcgcagggac attaactttt
     3001 gcgaaagaga agattgttct tgaacttgca ggggtgatga aaaaattatc taaatacaca
     3061 tacaaagtag atggtgacga tgaaggtgaa gtgaaatacc gttctgttgc tggacgagtg
     3121 tatctaattg ttaatcctgt tgattactgg gatatcgtag ctagagttac atttgcaaat
     3181 ttaaacaatg tgtatgggac tacgatgcca tttatcgctg ttgatcatat catcgaatct
     3241 gtagacgtac cagaaagtaa atgtattgca tacgttgata aagagtatga agctactcaa
     3301 tcaagagctg aaaaagtcta tgtatataaa gaaacttttg caatgaaacg cgcaactttg
     3361 tatgctgtcg acatgcttgg taatggctat ccaacaaata atgatgcagc acaagtatat
     3421 gacttggact ttacagcggg ggagtaatgt cccctgcaat tggtgagata acaccgacga
     3481 ctgatggggc agtcattaat ctttcgtaaa tggggttaga gtatgaacac tgaagaattt
     3541 ataaaagaat ataaggcccg atttcgtatt ttccatactt ccgaagatga ggatatcggg
     3601 aaacagttag agagcgggtt tgccgatata aaatctctaa tcggcagttt tgatccttcc
     3661 acttatgaga aaggaaaaga attagtttat gaacgtactc ggtatctacg gaacgaatca
     3721 ctagagtact tttacgacaa ttttcaaaca atgattatgg atgcatcaat tgatctggca
     3781 ggtgctggat atgccaatta aatcaaatta taagaagcct aaaattgtag ctggtgatct
     3841 aaatacgcca gttacttttt ttgagtttca acctaataag ggtccagaac ctggtgatga
     3901 agaaaaaaag gaactgtatt tttgtacagc actagtttac aacccatcaa tgaaagatcg
     3961 tgacattctt ggcacaaaag aaggcgttac gataaaaata cgggatcccc atcaatctta
     4021 tattcctact aacaaacata aagtaaggat attagattat cgatatggag ataaagtttg
     4081 ggaaataatc gatgtctcac cggatttgga agaaaattca attgtaaaga tagtattaga
     4141 attaattact taaatttggt gataaatgat gagtaacgta aagtttgaag gcgttcaaga
     4201 aacaatccaa gctttagaaa aaaagtatgg agaaaagaaa actaagacat taacaaaaaa
     4261 agcaatcaat gttggcgctg aaaaagtaga aaaacagttg caaacggaca tgaccgtttt
     4321 taaagacaaa ggttatacga tcgctgaggt tgttcgcaag aacgctacgt atagagatta
     4381 caaggcagaa gctgaaatcg gttggaatgg tccacatcaa cgatatagaa tcattcattt
     4441 gaatgaatgg ggatatacac gaaagggcaa acaaattaga ccgagaggat ttggtgtaat
     4501 aacaaaatct cttaagaatt ccgagctgtt gtactttgat gctgtaggag aggaggttaa
     4561 aaagagttta tgaaagatat gcttaatatc atttacgagg ctctttgttt gaatgagtat
     4621 attcattcaa tgacctacaa cgagaatacc gagcaatatc gtattaaatt ttatgagcaa
     4681 cctgaaaccg ccgataaaac aggtccaatg atcacaattc gtcctgtaga tacacctaac
     4741 gaagcgtacc acggaagtaa caaagagctt tctgtggagc atatgataca gattgacgtt
     4801 gaatcgaaat atagagcaac atgcaagcag attcaatatg agataaaaaa agagatgaaa
     4861 aaactaggct ttggccaaat ttccggtcaa gggttagatg aatactttac tgaaacaaac
     4921 cgctttgtag acgcgcgtcg ttacgaagga aatacaaaaa tttacgatac cgaatattag
     4981 aaattaagac acgaaaataa cgtgtctttt tttattaccc aaaattagga ggaaaatttt
     5041 atgactttag taggattcaa aaaaatgaca atcggggtct ttgacagcac agggaagatt
     5101 ccagcagcaa atttatatgt aattgaaggt gaacaaaaca aaggggcgac tgtatctgct
     5161 gaaatcagtg ggctatcaaa agaaccatca aaagtttacg gatcgaacat cgcttactat
     5221 gtctctcaaa aaggaacagg cgatgtttca gctacttttg gattgttaga tttaccaaca
     5281 gaagtaaatg acaaaattct tggttacaaa accgatacaa acaaaatcag cttcttggga
     5341 gaagatactg agccaccata ttgtgcaatt ttgatggaat cggaggattt gaacggcgat
     5401 acagcaatgc ttgcgatgtt caaaggaaaa ttcagtcgtg aatctattaa tcttaacact
     5461 acgacaaatg aagctttcga accagaagca gaagaatatg tattttcagc gattgccaat
     5521 gatgttgaag gtgatgctaa aggccaaaca gtcgcaaaat atgttggaga cgaagaagct
     5581 tctattactg gtttgaaaga gatggtattt cctgcagcgg gggagtaact agccccgttg
     5641 ttggagcaat cactcccact attaatgggg caacagttga attaagttag gaggataact
     5701 atgccagata catttaaaat ctataaaaaa aatggtactg aatttacaaa agtcgcagag
     5761 ggtgaaagtc cactttctat tactggaatt gcagcaaata ctcaagttgc taaaggagat
     5821 tatcaagcta cgcgtgtggt cggaggtcaa gaatcagtaa aagtagatat tcctgcattc
     5881 accactcttc caatttcagt tactggtgtg acgttggata aaacgacagc tgaagttgaa
     5941 caaggcggaa ccttaaaatt aactcctact gtaacaccag caaatgcaac agacaaaaca
     6001 ggaagctgga gcagttcgaa tacagctgta gctacggtta ctggaggcaa cgttacagta
     6061 aaaacagacg ctgaagttgg cggaactaca gaagtttcat ttactactac ggatggcggt
     6121 aaagtagcaa aatgtactat tacagttatt gaaaaagcag cgggctagtc ttcggactag
     6181 cttttttatt tggaaaataa tggaggaatc taaatggcaa aagtaagaat tgaattgaaa
     6241 gaagaatcag gaaagaaagt ctatgaaaac atagacacaa cagggaaaga ttatcgaaaa
     6301 gctttagaaa caatcaaaaa gctaaatgaa gacggagtaa tgatttggaa tcgtttagac
     6361 atttatttgg attttgctgt aggtatcttc agggatagca aattgacagc tgaacaaatt
     6421 cttgatggct taccatctga gaaggtaatg agcacattgg acgacatcct aggagaagta
     6481 atggggattg aaagtaatcc agacccagac gcaaaaaagt aacacccgaa gaagcagaag
     6541 aaatgtactt aaacctatgc agagagttag tgaaagcggg atggtcacta tctgacatag
     6601 aagataattc ctttgaaatg atgatgaaaa ttgcgtgcac ttcgcctaag aaagagaaac
     6661 aaaaagaagt tgatcttaaa gacttcttga aatccatgta gagaggaggt aaacatatgg
     6721 caaatggaaa acctttaggc aatatgatta tcaaactaga cttagacagt tcagcttttt
     6781 caaaaggtct agccggagct aaaaacgctg ttaaccatca aatgaaagca atgaaatcac
     6841 agatgcaagt aatgaactct tcaggaaatt ctctcggtgc tttacaagca aaatacaatg
     6901 gtttaggcgg agttctacaa gcaaatgaaa agcaagtaga attgctcact aaggcttata
     6961 aggatagttt tgactctaac ggaaacgcta ctgcttcgac tgctaaatac gctaatcaat
     7021 tgaatcaagc aacagctaga tcagcaagtt acgaatctca gatgaaaaca actgttggac
     7081 agattgctcg gatgaaagtg gaaacggaag ggtttacggg gaaactaaag gctaattctg
     7141 atgtattgat taattctggg aaaaaaattg aagcctttgg taaaggagtt tctgggatag
     7201 gatctgccct aactgttgga gtaacagctc caatagtggc aggcgcaact gctgttacta
     7261 gtgcagctat ttcatgggaa agtgcttttg caggtgtaaa aaagacaaat gatgaagttg
     7321 tagactcgaa tggaaacgta gtttattcct atagcgactt agagaaaggt ctgcgtaatc
     7381 ttgccaccca attacctggt agccatcaag aaatagcaaa tgtagccgaa gcagccgggc
     7441 aattaggtat aaagactgaa aacgtagtta gcttcactaa aacgatgata gacatgggtg
     7501 aatcgactaa tatgtcggct gaaactgcgg caacttcttt agcacgattt gctaatatta
     7561 cccaaatgtc tcaaaaagac tttgataaat taggatcagt cattgttgat ctaggtaaca
     7621 attttgctac aaccgaatca gaaatcactg aaatgggatt gcggttagcg ggagctggta
     7681 aacagattgg aatgagccaa ggcgaaattc taggatttgc tactgctcta tcttctgttg
     7741 gtgtcgaagc tgaagcgggt gggtctgcct tttcgaaggt catgatccag atgcaactag
     7801 ctgtagaaaa aggatttggc gcttttgatc agctgaaaca gatggcagaa gaacaaggtg
     7861 ttccgtgggt taatttagta aatgctgtga gagacggtgg aaaatcgtta aaagcagtat
     7921 cagagcaaat gggattcact tcatctgatt taaagaaaat gtataaagaa gcggacaatt
     7981 cgaaaagttc gcttgaaaat tttgcgaatg tggcaggtgt tacaagtgaa caattccaga
     8041 agttgttcaa gagtgatcct tcagaagcaa tcatcaagtt cattcaaggg ctgaaagatt
     8101 cagaaaaaca tggtacttca gcaatcaaag ttctggatga catggatata aaagaagttc
     8161 gactacgcga cagtttattg agagctgcaa atgctagtga catattcaaa ggagcaattg
     8221 aaaaaggaaa cggcgcgtgg aaacaaaata cagctttaac aaatgaagct aataaaagat
     8281 acgaaacaac tgaatcaaag ctaaagatat tgaaaaacga agtgattgat acagcaatcg
     8341 atcttggagg tccatttgta gacgcgttgc gtgatggtct aaatgtatcg aaaccattga
     8401 taaaaaactt agggaatatg gcgaaagctt tctcagctat gagtgatgat caacaaagaa
     8461 acattatcaa atgggttgga ttagcagcag cagctggtcc agcgctgaag cttttaggaa
     8521 ctggtacttc agtaataggt aaagttactt caactactgg aagattgacg aaaagccttg
     8581 ttgatttagc agcgaaagca gcagaaaaga aggctatgga tgcctttgca tcgtcggtaa
     8641 caacagctgg tgctgcagct gcaaagacag caggagctgg aggtattggc gggttagtcc
     8701 ctatgatggg aaaaacagct agtgctgcaa gtactgctgc gggcgccagc ggaataggcg
     8761 caatgacaac gtcactaggt ttgttaggtc ctgctctact tggtattgtt ggcgttggtg
     8821 gcgcactggc agttggctat ggagcatgga aattatttgg tgaagaagct tggaattcct
     8881 cgcaaagagt aaagcaatgg ggaacagatg tcggtgagga gatagatagt actcttgacg
     8941 gagtacagaa gaatattgaa ggtgcaaatg gtcaattcgg tcttctcaaa gagggtttta
     9001 ctgttgatga tgcaaataat atggcggcta atttcgcaac cgctggaaca tctttggaaa
     9061 catctttaac gggtagaata aaggcattag acgatgcact aaaagggcta cctcaatcag
     9121 ttcaggatgc catgaaagaa gtagctgaat cagaagaaga aaccttaggg caatctcttt
     9181 ctaaaataaa agacaacaat gaagaaataa aaaaaatcag agaaagagcc gcaaatcaga
     9241 acagagactt gactgtttca gaactacaac agatacagga tctaatgaaa ggaacttctc
     9301 aagcgtatgt ggatacgctc gatgtttctg ctaaagacag aaaaaagata ctatcagcca
     9361 tgaatggtga tgttgcacaa gctacagaag aagaagcgaa gacgtggtta caatctttag
     9421 cgaaacaaaa agctgcaact accgaaaatt atagacaaat gcttgaagaa aaaaaggcgg
     9481 ctttaaagga aaagggagta agcaaagaat tcattgatgc attagaagat gaaatgaatg
     9541 attacatcaa aactacaaat caaggattcg atagccaaat agctgctata acaaataaat
     9601 atccacaatt gattaacgaa gtgagtttat caaatggtca attatttagc aaaacggcag
     9661 ctgctatgga caatactggt cagtatacaa aagtttttct cgaaagtaat gaaaagattt
     9721 tagcccaagc gaagaaaaca tcggatgaaa tagcagataa tgccgaaaaa aatgcagaac
     9781 aacttggtat ggtagctgat gaggctacgc atgctggatc aacttggaat ggcttagttt
     9841 tagacgaaaa aacaggaaaa gtaaaaacta atgtacaaga agtagtgaat gaagcaacga
     9901 aggatttcac taaatggaat gaattgaaac ctgttgttca tgatgcgaaa ctaacaacga
     9961 atgcaaaaga tgtcatttcc atagcggcag tacaaaatgg ctattgggat gatatggaat
    10021 ggaaagaaaa agaagcatta ttagaagatg agtgttctaa aaatgtatac aaggctttag
    10081 aaaatgctgg taaatggaat gagttagata ttccttctaa agaagcaatc ctaacttcaa
    10141 atactcctga agttatgggt gaaactctca tgaatttggg attatgggat acttacaaac
    10201 ttcaagtgaa agatttagat ttgaatgcgt ataaagtata cgatgctatc gatggatcag
    10261 aagaaaaatt aaatagttgg gctcaattag ataaccccac aaaagaattg ttattagata
    10321 actcagattt ttctacaaaa ctttttaatt ccgaagaaat gtttaaacgt tggaatcagt
    10381 tatcatctaa tgcaaagttt ctttcggcta ataatactaa atttacagag gttcttttat
    10441 catctgacag aatgtggaat caatgggtaa accttcctga taatgagaaa agattacttg
    10501 cagataacga agatttagct accactgttt tatcttcaga agaaatatac ggaagatgga
    10561 ctcaattacc agataatcaa aaatatatgt tagctgacaa tactgactta gcaaacaaaa
    10621 tattcgcttc agaagaatca tggaatgcat ggaaagcaat tccagacaca gttaaaaact
    10681 tgcttggtaa taatattgat ttactggcta aagtgaaaga cgggactgta agtgtagaag
    10741 aatataacaa agttatgcca cttcttaaaa ctctatatgg agataacgtt aatttaaaag
    10801 aggcaatcag tggggctaaa agctctatag agaattacaa caaaaatatt tttcctagtg
    10861 ataaaactgc agtaggacac gatgaggctt cacaagctgc taaagatgca tttgaagcgt
    10921 tcaacatctt ccaaacaaaa ataccagata aaattactaa aactgtatca gctgacttta
    10981 taggtcctat gccgaaaaat gcgaaaggaa caaacttcca tcctggcgga gcagctatgg
    11041 taaacgatca aaaagggcct acttatgaag agcttataac cttgccaagc ggagaaactt
    11101 ttatacctaa aggacgtaat gtcgttttag acttaccaag aggttcgaaa gtactgaacg
    11161 ccactaaaac taaacgtcta gtgcctaaat atgctgatgg tataggaaac ataacgacgg
    11221 tttcatctac gctaaactta gatgctttaa ttttagctat taacgagcta acgacggtat
    11281 tgagaactca actgatggca ggaacgactg atgctaaaga tactaaacta tcagatgaaa
    11341 caaaagttgc cgatccgatt attccggaca gtctttctga aaaatcagat cagtatctag
    11401 gaattggagc tcaatggtta actaatctaa tgaatggttg gaactcagcg gttcctcaat
    11461 atatgaatag tgaaatggtt tttattacaa actatctcaa tgcgctaaaa cttcaaaaca
    11521 atcctaatta tacgcaagga gcaacatgga ataagaactt actgaatgga tggaatagtt
    11581 taactgggac ctttattgct acaatcaatt cattttgtaa tcaagcaatg gtcacgctta
    11641 gaaactacaa cactcctatg tacaacaatg gccgaacgtg gcagcaaaat aatcttaatg
    11701 gttggaattc attgtatgga tcattcatag cccgtgtaaa tcagcttggt aacgattcga
    11761 ttaacaacct tcgttctaaa agtggtggtt tttacaatgc agggacattc ttgttgcaat
    11821 ctttaataaa cggaatgaat tcgttaggta attctctttc tactacaatg aacaatgtcg
    11881 ctaatacaat ggttggtggt ataggcaaag gagttaatgg cgtcatatcg ggagttaact
    11941 atgtgcttaa ggaagtcgaa tcaagtaaga atatcggaaa ctggccaatt ccacaatacg
    12001 ctaagggaac tgatggacat cctggtggac ttgctatgat taatgatcaa aaaggcctag
    12061 ttcacgaaga atatgttcag atgccagatg gacgtagttt tattgccaaa ggtagagatc
    12121 ttcttgttaa tttgcctaaa ggtgctcagg tcttaaatgc ttctttaaca aagaaattaa
    12181 aggaacgttt aaatattcca cactacgaaa aaggtattgg caatttagat attgttgatt
    12241 tgttagatga tgaaaaaaga atgttagaat tcttaactag caaagttgat ttttcaggca
    12301 ttaatgaacc atggcttgat atgactaaat caggaacgtc attaatgtcc aaagctgcaa
    12361 atacaatgct tcaatcaaaa ttaagtgaat tcttcactca tggcaatttt gatggtgcag
    12421 taaatgccaa tggtgtttat caatatttag ttgatgtggc acagaaagta atgggtaagt
    12481 tccccggact tacagtaact tctggatatc gagcaggaga tgcctactat cacggaaaac
    12541 ggcaagctat cgacttggct tatccaggta tttctggaga tccgagatat acagcagcag
    12601 caaattacgc ttttgaaaaa ttcccttcaa aaattgcgta tgtcattacg aatggacgtg
    12661 tacgtgaccg catgggatta tctggaacaa ggtcaagcgg acaatggacg aattggccag
    12721 atggcgatca cttcgatcat attcatttaa acggttcgat ggggtctggc gatatattca
    12781 atggaggagt tggaggaagt ggtgttgcac gctggcgttc ttatgtgtct aaagcattga
    12841 aaatgaatgg attaccagct acagcagcgt atataaatgc ctggatgtcc caaatccaaa
    12901 cggaatctgg tggtaatgag aaagcaatcg gcggcaatga tgggctcgcc gaaggcaatg
    12961 caacaggatt acttcaaacg aaacccggaa cttttgcagc taacgctttt cctggtcacg
    13021 gaaatattat gaacggattt gataacatgt tagcagcaat caattatgct aagaaacgtt
    13081 acggagttgc aggtatgttg caagttatcg ggaaaggaca tggatatgcc aatggcggct
    13141 tgattaccaa agacggacta tatcgagcag gagaaggaaa taaaccagaa atggttattc
    13201 cattgacaag aaaaacaaga gctatcgaac ttatgggtca agcattagcc ttcctttctg
    13261 gaagcaataa aaaagaaacg aaaagtgaga ttaattctgt agataataga catatcctac
    13321 aagcgataga aaaacagtct cgtatgacaa atgatctgct atcattatta ctctcgtttt
    13381 ttaaaagtaa caatcgatca gataaagaac tagctttaga tattcagaaa attttaatca
    13441 ggaggacata gtatttgagg acagtaatat taagaaataa attgaatgaa gaaattgatt
    13501 tgtccacaga agattatttt gctactgaaa tgtcgaatct aggttttgaa gttacaaaag
    13561 agcatattgg acaatggggg agtttcagag aaataggaga gagtattgaa ttgtctgaat
    13621 ttcaatcatc agttatgatt tctgttcacg gatttagaga acaacaatta tatggttcat
    13681 tagttgaatt tctggctaat ggaccttttg aactagaatt ttcttttgat tcagaaacaa
    13741 tgatcaggaa atgtagtttg aaatcattat ctaaaacaga aattgattcc caaagctcat
    13801 tgctaacaga tactttgaat ctatatttta cttcaagctg gtattctatt aagcgtgaaa
    13861 aattaataca gaattcaact tctctcaata cacgtggaaa agtttactca tttgttcgac
    13921 catatgtcta caccaaaaat gtatgggaaa agaaaggtgt atttaagttc agtaataatt
    13981 ctgtatattt gacaaatagt acagagcgaa tgtctccgtt aaaaataagg attattggag
    14041 agtgctcaaa tccatattgg gaagttatcc aaaactctaa agtagtagct tcagatggct
    14101 attttattaa tatgaaagac acacaaatat tagaagtgtc tagtttgcta gaagaccaaa
    14161 cggcaatttt aaaagatgta ggtggagttg aatcttctgt ttatcaacaa caagatttta
    14221 ctaaatcaaa cttcgtccaa gcgcctctag gagaattcag cgttgttttt catgttggac
    14281 atgcaaatgt agagattgaa ctttatgagg agcgtgatct attttgattt tagccattac
    14341 cctctttcac cgagatatga atttatataa cgaacgttta atttcgactg agttcgaatt
    14401 tggaacggat gaaattggag aagaagtaag taattttatt acagataaat atgtccatgt
    14461 aaaatcagga gattttctac tagcaaaatt tattcctagc gggaagattg cttattttgg
    14521 agtgattagc tcgcaagaag acaaaaaaat aaattgcaaa ggattactca gtttggccga
    14581 tagcgagata ccgactgtaa aaatatcagg tgctaattat gaagagcata ttcgacgact
    14641 gattgaatac tacttattga acgatccaac aaaacaacta aaaaatattt tggaggtaaa
    14701 aacagaaagc gttacttctc attcttatca agctgttgac acaaataaga gacgattgaa
    14761 tgcatatata ataaatgggt tcaaaaaata taatgttaaa tggtatgtca aagagattaa
    14821 aggtaaaaaa atatatactg gtattcgagc agttgatgat tcaatttata ttaaagataa
    14881 ttcatctgag tttaatgact gggatgtttt tgtgcaatct cctggaccag ggaacgagaa
    14941 caaattattg atcgtagata aagcaatgaa agatatagaa aatcccttgg tattgtctac
    15001 gtggtattta gatgacgaaa ataatcttac gcaagattca acgaataaga acattgcatt
    15061 gcctaccgtt aatttagtaa atatatatga tcaaactcaa gaagatagac cttcttatga
    15121 agaggtagct aaatcggagc ttaaaggcaa cgcctattca catgaaatta aagttaatgt
    15181 tgtgctaaat gctagaaata tcaatgtaga aaatatcgaa acaggaatgt tagcgactat
    15241 tgtttacaaa gagcagactt acaaatctgt tctaactgcc tggcgaatat ccagcagtaa
    15301 aaagaatata gaattgacat ttggtaatgt ccgaagtcgt tttatggatt attttgaaga
    15361 taatggggga taaaaaatgg ttagcaatgt ggatggatat caatttgaaa acgtgaaagt
    15421 aagcgcagaa aatgatgcta gactttatca cgttttatat aatcggaaaa atcaggttat
    15481 tgatggttac gatcagtcta tgaatttatc ttcaagcgga ttaacagtaa aagttgctgc
    15541 aggagcagcg attattcaag gtcgtatggt cgttgttcga caagaagaaa gtataacagt
    15601 tccagcaaac tcaagtggtt atatagcatt aacagtggat ttgacacaag aagttatacc
    15661 tggatctatt cttccagaat cggaagaata tgaatggact aataatcaag tcaagctaga
    15721 atttataaca aaagttataa aaggtaattt aaacaatggt gataaggtct ataatttacc
    15781 actatgctca gttaattcta ctggatcaac tgtttcaatc tcaaagatat cggatagtta
    15841 cgagctgact ctctctaaag gagaaatttt gtggaggggg actgcgttaa tgcatgatac
    15901 tcaaactgtc caaccttcaa aaaaaatttg gcagacagtt tcaggttttt tgttagtatg
    15961 gctcccatac gaaaacgggc aagcaattga ggatagatat gtaactacgc ctttttataa
    16021 ggagcgtata acttttacta atgttttagg agaaattgtt tcaggatttg atgactatca
    16081 caaaaagtgg tttagtaaac gaataaacta taattcgaat actaatatat ttacaggtgc
    16141 agcaagcaat gcaagtggag ataacgcaaa tatggtgctt aggtatatag tttcttttta
    16201 gttaggaggt atggaagagt ggcaaattta gaaattaaat tatctgcaaa taagagacag
    16261 ccttatctac gtcaccgtgt tgttggtaga gttggtgatg gggggctcac aacaatcaat
    16321 gtacaacttc ttgaggaaga tgaaattaca ccttttgtaa ttaatctaaa cggtactttg
    16381 aaatttgtgg gcgaagtttc aaacggtaac tataccgagg gagaaccaga aataatcgat
    16441 tcgactaatg ggttaattag ttacacgttc actaagtcaa atttcagcac gagtcatcaa
    16501 ttcaaacaag catattttga atatgtagat cctaacggca aaaaagtaac ttttcagaac
    16561 ttcatcatag acgtgttaga acgagtagat attaattcgg agcaagcgaa atactatatt
    16621 tcttcattgg aaaaattaca gagtgaaatg caaaccactt tcaatcagtt cattagtgat
    16681 aaacaggtcc aatacgatca aatctactcg aaatataacg aattagtaag attgataaat
    16741 gaatcagata agcaagtaaa tgatcgtatt gataaggcaa ataatcgtat tgatcaaacc
    16801 aatcagcaaa tcggcgatct cggcaagctg aaaaagatgt acagtaacag cattgatttc
    16861 gggggatatg attatagcgg taagccgaat ttaatgtcca aactaaaatc gagcgatttt
    16921 aacgttggtt accacgggtc actaacttcg gataacgaaa agctacattt tacttctgat
    16981 ggtacaggaa gcattattat gtttacgcgt attaatacac ctcagcttgc tagtgggaaa
    17041 acctatactc tgagtgcgaa agttcgattt gatgaaggaa ctacaggagc tattgataaa
    17101 ttacgtttgg tgtatcgtac atcaccagga gaaaagatat tattggaagc aaatagtaca
    17161 aatattacaa cagatgatgt agggaaagaa ataacaatca aaggtacagc taacgttaat
    17221 tatcaaatca caaatttaga tcgattttat atgagtatta gctttgttga cagggataaa
    17281 ataaatggcg gatttaagtt gtacgacatc aaaatcgaag aaggctcaac agccacaccg
    17341 taccagccta acttactaga tgacccttac tgggtaggta aaacatcgtt gggtgagaat
    17401 attgcaaata aaggtacgac gttcccaatt aaatctagcg cgtacgaaat atataaaggt
    17461 aacacggaag aagagcttat aataggtcaa acgtatacta tcacgcttaa aggaacaaaa
    17521 cccgcaagtc aaacctttgt agcatataat tattggaacg ttaattttgg agaactaaaa
    17581 ccagttgagg gattgacaga cgtatggtct ctaacattca caccaacgaa acttgaaccg
    17641 ggtttgccta aagaccttcg catttttcag ttgcctaaag aaacggccgg cgcatgccaa
    17701 attgactggc tcaaaattga aaaaggcgac acccgaaccc cgaatattag tcagtttaaa
    17761 tactttggtg aaggattgaa agatagcaac aatccgaacg actacagctg ggacatcacg
    17821 cctgaatata ctgaaaaagg cttgaatgat acggttagtt tgactgaacc gcaatccatc
    17881 gaaggattga aaaatttcga agatggtatt caatcaaaag gaaaatctgt attgacatca
    17941 gacgacaaca aatatgaagt cgtaacctta acagttacaa acgggaatac cggatcagca
    18001 aagctttatc gtgaaggaaa aaccgtcact atttattttt ttgcgttgaa tgggaaaagc
    18061 agtggtggaa atgattcaac gatactaaca attccagaag gctatcggcc accaattagt
    18121 tttgagcaac tggttggctc gatagaccgt tctactttga acagtgctca gttatctatt
    18181 ggtgcagatg gagccattaa atggcgaaga aactcaagtt atggatcagc ttattcattt
    18241 gttatcactt attcaattta agggaggaaa tctaatgaaa gtagtttaca aatcaatcaa
    18301 gccttacgga ttcgagcaaa tcattgtgaa tgacgaagaa catttaccgg aagaatgcac
    18361 agaagtcgaa ccaccgattc caaattggaa accgaaattc aattactggg agggaaataa
    18421 atgaaaaaca tttggaaata tggccgtact ggcggagagt atgcaggaaa agtattggac
    18481 gacatgcttg tatccgttcc ttacacagat cagccaccgc tcgaaggggt tcgttcagat
    18541 ggcgaaccgc taacgattgc tgatcagatg tttgatccta aattgaacca atggattatt
    18601 ttagcgaacg cgttagatca caacgattta aacaatctca aagcgatgta cgaggctctt
    18661 gaacatgaaa acggcaacct aaaacagcta aatgccaaac tcatgctaag cgatgtagcg
    18721 attaaacagg aaaatactgc attgaaagaa aaagctgaca gtttagcaca aatcaattca
    18781 aaaatgatgc ttgcttcgtt acaaaatagc aaagacattt cagaaattaa agagcaacta
    18841 aatccagctt caaaaggagg tgagtagtat gtttagtttt agcgatgtga aaatgatgta
    18901 tgattggggc tgttttactg acgatcaagt tcgactattc gttccactat gcattacaga
    18961 cgaagaagca gataaaatca ttaataaaga taagagcgca tcttaattga tgcgttttta
    19021 tttaaggtaa aggagttgtc acatgattaa tttaggggaa tggggaatga tagcaggatc
    19081 aataaccgct atcgtttctt tgattttatt agtaattaga ccgattgctg catctttctc
    19141 gaaaattact gagactcttt caaaagtaag ccacaattta gatttgctga ctaaagattt
    19201 agaatcgagc aaatcagatc gattgatgat tcatgaagaa ctaaagaaac acgatgaaag
    19261 attagataca catacagaaa aattggtgga acacacgcaa caaatcaaaa ctttgtttag
    19321 ggagagaaga aaatgaataa taaaacgttc gaagtactaa aatggttcgc actggtaatt
    19381 attcccgcac tagctacttt cgtggggtta gttggtaaag cgctcaattg gcagtacaca
    19441 gatatctgtg ttgtcatcat tactggtttt ggcgcgtttt tagggagtgt gttgggtgta
    19501 tcaaatcgaa cctacaaaat gttctcggct gaaagcgaag aaggaggaaa caaatgaaaa
    19561 agaaaattac tattactgcg atgagcctgt taacggctct ttttttattg ccaattaacg
    19621 gatttgccta tactatcaac aatgaattta atttgggcgc aaatgaaggt agctcacaag
    19681 tagcaaataa tcagtatatt ttactgcatg aaacggctaa tgaaacagca acaggacgca
    19741 atgaagcgca gtatatgcaa cgttcatgga ctagcgctta tactgcttat attgtgggag
    19801 acggcggaat tgtttaccaa gtcggacaac ctggttatgt acagtacggt gctggttcat
    19861 atgctaatgc caatagtcct gtgcagattg agttacaaca cacacatgat aaagcaactt
    19921 ttgaaaaaaa ctataaagca tacgttgaat tggctagaga ttcagcaatg aaatatggta
    19981 ttccattaac attagacacg ccttataacc aaccaggaat caaatcacat ttatgggtaa
    20041 ctcaaaacat ttggggcgat cacactgatc cgtatggata cttatcagaa atgggtgtaa
    20101 gtaaggaaaa actggcttac gatttagctc atggatttac agacgaaaac ccaacgactt
    20161 ctgagaacaa gcctgtcatt gatccaaccc gagcaggtgc agcaaatcca actttatcag
    20221 atggaacgaa ccattctcac attgatcagt ttggagaaat cgaaaatgcg aacttacacg
    20281 tcgctggatg gcacatcgct aactataagt acgagtatat tttcattatg gactacaata
    20341 ctgggaaaga attagctcga gtaagagctg atggaattta tagaccggat gtaaatcaag
    20401 cttataatac ttctggaaat gttggctatc atgtatcttt taacatgcgt aacttcccta
    20461 ataagaaagt ctatgtcatg atgcgggcaa cgaatgatcc agagggaaac actaaaggcg
    20521 gtgcgcaaga tttccatgac aaacgttggt atttaaatat tcctaaacga taaaaatagc
    20581 tcctcgttga ggagcattac ataactatat tgacaactat aaaaatcatt cgataaaata
    20641 gtgatgttat cgcatatctt cactatcacc cataatagtc acactccaag ctatgcgata
    20701 acaggtttgt tgccacacat tctactggtt gattgtttat ggctttatgt ggcaacaacc
    20761 agtaccctta gctcagttgg tcagagcag
//
LOCUS       DACDKM010000041        19476 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20834_2016
            SAMN12640370-rid9891663.denovo.041, whole genome shotgun sequence.
ACCESSION   DACDKM010000041 DACDKM010000000
VERSION     DACDKM010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640370
            Sequence Read Archive: SRR10040891
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19476)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19476)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742835.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742835.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:04:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,012
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 29 of 75
            Pseudo Genes (incomplete)         :: 33 of 75
            Pseudo Genes (internal stop)      :: 19 of 75
            Pseudo Genes (multiple problems)  :: 5 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19476
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640370-rid9891663.denovo.041"
                     /isolate="E20834_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(22..996)
                     /locus_tag="IV334_001412"
     CDS             complement(22..996)
                     /locus_tag="IV334_001412"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HAP8943490.1"
                     /translation="MSNKYQDDTLKIFSLNGNRPLAEKIAKVFGTELGKSVVKQFSDG
                     EISINIEESIRGDHVYIVQSTNAPVNDYYMELLIMIDAMKRASAKTINVVLPYYGYAR
                     QDRTAKPHEPITAKLIANLIEEAGATRVLTLDLHTVQVQGFFDIPVDNLFTMPLFAHY
                     YRQLGLTGDDIVVVSPKNSGVQRARSLSEYLNSTLAIVDHADDASTEGGYVIGDVQGK
                     TCIMVDDILNTGATLARAANVLKENGAKEVYACASHGLLSDHAKSILDNAPIKDICIT
                     DSVFTEENRHPENLTIVTCSELMGEAVKRIHENTPMSPLFRLEEKEFE"
     gene            complement(1076..1951)
                     /locus_tag="IV334_001413"
     CDS             complement(1076..1951)
                     /locus_tag="IV334_001413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide dehydrogenase"
                     /protein_id="HAP8943491.1"
                     /translation="MKKRRRSQLNQVVDKPLYFKVDKKIKKLASTQQLQSRKSKLLFL
                     ALVFEDQSYVIIDQSGHPVEYSPAKYTYQEGLSCKKWKLINEEPIELSRWINRKEDIP
                     VLIEEKRSGKELANCWVGLPEERFLRYKKWATPSGYLCGTYAAAVLLAYYQDYRKGWM
                     LPNEIRKKNTADSLVLTKALRSQIQPLGLPTIPFQVSTGISSFLKKNGNHERARATLL
                     GSWQRATKRIREGKPVMIGILKVLGSTYGNHWVTAYAYFETETGERYYKVHDNWGDYH
                     KVIPASWSNGTVSLP"
     gene            2081..3988
                     /locus_tag="IV334_001414"
     CDS             2081..3988
                     /locus_tag="IV334_001414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M13 family peptidase"
                     /protein_id="HAP8943492.1"
                     /translation="MNKERLKQDFYEAVNEEWQKTAKIPADKPATGGFQDLVDGIDQL
                     LMGETDDLLAHPEKVSSEKMKHFLAYYQLANDYDRRNELHAQPLQPIIEKVESLQSFD
                     ELNKQFPEWLLDTLPLPFNLDVDADMKNAQTNAFFAYPPSLFLPDKTYYASDHPNGAQ
                     LIGVFFDMAVQLLELAGKEKSQAEAITEQAVQFDKLIAPHVKSAEENADYSKMYNPQS
                     FDTFIKYTDKLDLKKLVTGLIGTTPDKVIVTDPVYFDHLNDILTEAHFPLFKSWMIIK
                     TVRSLSGYLSEEFRQVSGIYSRVLSGTDEAMPQKKAAYYLASGQFDQVVGDYYGKKYF
                     GEAAKKDVEQMVKKMIAVYKKRLEENDWLGDATKEKAVIKLNNLGIQVGYPDKIPALF
                     DQFKTVPADQGGTLLSNTLVFSKLTLKDRFSKWNKPVDRSEWEMSADTVNAYYHPFRN
                     VIVFPAAILQAPFYSLEQSSSANYGGIGAVIAHEISHAFDNNGALFDEYGNLNNWWTE
                     EDLTQFQNKAQAMIEEFDGLPFADGKVNGKLTVSENIADAGGLSCALEAAKTESDLSI
                     EEFFVNWATIWRTKAKKEYQQLLLQIDVHAPAKLRANVQPKNLDEFYETFGITEQDAM
                     YLAPEKRVRIW"
     gene            complement(4529..7807)
                     /locus_tag="IV334_001415"
     CDS             complement(4529..7807)
                     /locus_tag="IV334_001415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1565 domain-containing protein"
                     /protein_id="HAP8943493.1"
                     /translation="MLKKRIKYSALMMNLLMLSTPVLISVVEVAANEQQSVNEENAHV
                     NIEGMSTMEEVPRTSEDMDTEEGNDPMMKESENEKTKKQSEEANEEWKGVVFGESTSA
                     SRYAIEPLEDGVRLSSTNNGGKFQSSGSDGMTYYYQAIPKDINFRMRATIEIESWTYT
                     NAQEGFALMVRDAVPKNHLFGQAFYSNSFAILGSRIEYVWDSDRQEVVNTSGSKYTMR
                     LGLGTRAITGISSEDPQAAPAPGSVSVETTPLETSAGFLEKETGSYNIFGNGHGNLFP
                     ATNSITKLDVEMKKTNTGLESYYYDPETGEEMASDIMYDWEKLYASDESVIYAGFAAA
                     RNMTIKVEGIEVAYSDPATDPPGQERPTRLIDPIYTVTSSNHSGNQDHTMSFRANADG
                     LLTIKNKATGEVLKNAKNLAITANREFDYQTKLMEGTNEFTFSFTPDKNYPPEEYVEM
                     TSYETKEILHTVDYRKPKYSTVYVTPEGSDAGNGSRQNPLSLTQALRYAYAGQTIVMA
                     PGTYSFERGLTIPKGVNGTRENPIQLIAEKNPENKRPVLDFKGTGNGMTLFSHYWGLR
                     GFDITNSAAMQKGLQISGNHNLIEEIHAYRNGNTGIQISGSSNDPFEAWPAHNLVKNC
                     TSFENADPGFEDADGFAAKLTIGEGNVFDGCIAYHNADDGWDLFAKNETGSIGKVIIK
                     NSVAYRNGWVPGIEGEGNGNGFKMGGSSLTGPHELINSLSFENLAKGIDSNSGTDIIV
                     NSSTSFNNGSYNVALYTSSAGNTDYHASGILSFRTENLEMREQIRPLNQNEDQIYHSL
                     NYYWNEREKQSENTLGHTISKDWIESLNTCSKEGQQPVTRYENGSIHVHGLMQIKPGN
                     RQTEKERVDGLPLSVGAIFDGETSPSSEYPEYAIVQEPNQTKPNEQNNPIIIQEANGV
                     AENAEGNIIELKPFTVFVSTFTPNQGTPVFEDMRYFPEKIQIVETQSSAQFSNHTAIF
                     SAPSKGEYTLEVTYRSEIFDGEKWSAHTESVTQQKAITVRETADVPPGNDKPERENPG
                     NESPNGKPNGKPSHPDRTAKESEKNGTQGLPKTGEFNNPLLALAGGILLIGVVVYVMK
                     QRKK"
     gene            complement(8078..8623)
                     /locus_tag="IV334_001416"
     CDS             complement(8078..8623)
                     /locus_tag="IV334_001416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HAP8943494.1"
                     /translation="MNALISIDYTYDFVAEDGKLTTGTAGQVIEKKLTAHTKKFIDQK
                     DFVVFAIDAHDPKDSFHPENRLFPPHNVIGTGGRELYGSLKSLYQDYEQEENVYWIDK
                     RHYSAFSGTDLDIRLRERQITDIYLTGVCTDICVLHTAVDAYNLGYKLHIFKDAVASF
                     DPVGHEWALRHFESTLGAEIL"
     gene            complement(8635..9327)
                     /locus_tag="IV334_001417"
     CDS             complement(8635..9327)
                     /locus_tag="IV334_001417"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HAP8943495.1"
                     /translation="MKIGIIGAMEEEVKILRENLSEPLSWERAGALFISGSLGNHEVI
                     VVRSGIGKVLASITTSLLIQQYGVNMVINTGSAGGIGEGLQVGDIVISDKVAYFDADA
                     TGFGYKPGQLPGMPLYYEASTYLRSEMVKAAKATNLNAKEGLIVTGDTFVDSPDKIKE
                     ILTNFPEALACEMEGAAVGQTARQFNIPFLIVRAMSDTADHSATQSFDEFIEDAGKRS
                     AEMVIEFVKHLV"
     gene            complement(9346..9630)
                     /locus_tag="IV334_001418"
     CDS             complement(9346..9630)
                     /locus_tag="IV334_001418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8943496.1"
                     /translation="MAKGPLITRSELRKRQQAQASESLKKQRKAETAYQQEEKKIASF
                     YRKESKKNKPITKTRISEREKTTKWNSFLMKSLIIVILMLCVVFLAIAFI"
     gene            complement(9643..10206)
                     /locus_tag="IV334_001419"
     CDS             complement(9643..10206)
                     /locus_tag="IV334_001419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HAP8943497.1"
                     /translation="MLNNEQFEEKTISRKEIYSGKIIDVAVDEVRLPDGGTSKRELVF
                     HPGGVGIIAFDEQDRLLLVKQFRKPLEKVILEIPAGKIDPGEGQNPEMTAARELEEET
                     GYRAKSLSHLTSMYLSPGFANEVLHIYHAQGVEKVENPLAQDEDEVLELYHLTLEEAQ
                     QAMKDQLICDAKTIYAIQYWELLTKGK"
     gene            10434..11096
                     /locus_tag="IV334_001420"
     CDS             10434..11096
                     /locus_tag="IV334_001420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-bromo-4-chloroindolyl phosphate hydrolysis
                     protein"
                     /protein_id="HAP8943498.1"
                     /translation="MIKRNWPWLLALVLLFLFVRNNSFSFVLGLLLIGVVLVLWGLFG
                     SRRKKGKQEEIPALTDELETHYEKSGMTANEISFFRQTMNQTKNEINQLQMNMQQTAK
                     LKAIDLRHDPVKAAKALFKELVKEPNRLHEASQFLYTHLPNLVDLTNKYNEINDHEIK
                     NKQTYEKLEESAQIIDQLSALIAQDYQKFVAEDLDDIDVEISVAKQSLKRDNKYEPEQ
                     KD"
     gene            11220..12416
                     /locus_tag="IV334_001421"
     CDS             11220..12416
                     /locus_tag="IV334_001421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxic anion resistance protein"
                     /protein_id="HAP8943499.1"
                     /translation="MDHKPTETKDVTPVNDTLEDLLNNPFSTPTDTLTTSQQAEIDQL
                     QEQQTAARLIDKLPAERQEQAKQLAAKIDANDAQSVISYGSAAQAKLSEFSQSMLNHV
                     QAQDIGPVGDSLTELMYRLQEANPDELRAGEGNIFQRVFGKVKQSIYEVTAKYQKIGA
                     QIDKISVKLDKEKDGLLKDNLMLEQLYQKNKDYFDALNIYIAAGELKMEELQTTIIPE
                     AMKRAEETGDQMDVQIANDYTQFLDRLDKRTHDLRLARQITIQQAPQIRLIQNTNQAL
                     AEKIQASIATAIPLWKNQVVIALTLLRQKDAVTAQRQVSETTNDLLKKNSEMLKISAI
                     ETAKENERGIVDIETLQTTQNDLIETIQETLRIQKEGKEKRRHAEVELGHMEEDLKQK
                     LLDLTQ"
     gene            complement(12517..13893)
                     /locus_tag="IV334_001422"
     CDS             complement(12517..13893)
                     /locus_tag="IV334_001422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAP8943500.1"
                     /translation="MDLFRKKEINLNHTSEMKKELKTSDLIMLGIGAIIGTGIFVVTG
                     VAANQNAGPALSLSFVLAAIVVILSGLSFAEFASRVPVIGGPYAYLYVVFGEFAAWLT
                     GWLLIGEFLLAVSSVASGWSGYMQGFLKSLGAELPQALTGGYNPENGTYIDLIAVLVV
                     IFVTYIVSLEAKKALRLNNAMVYVKFGIIALFIIVGIFFVKPDNWQPFMPFGFSGVLD
                     GAALVFFAFLGFDAVAMAAEEVKNPQKDVPRGIIGSILIATVLYIIVTLILTGIVPYT
                     ELGVNDPVAFAMRYVGHGTVGAVIAVGAILTLLTVTISMMYSLARLLFAVSKDGLLPK
                     FMTEIDQKHRTPKKATYAAGVAAVFFAGFFPLNVLAELTNIMALAYLMLVSLGLLKLR
                     KMFGKPKAGEFKVPFVPLLPIVSILTCIVLMLRLQTVTWIVFGIVMVIGLIIYFGYGY
                     GHSKMNEK"
     gene            complement(13930..14796)
                     /locus_tag="IV334_001423"
                     /pseudo
     CDS             complement(13930..14796)
                     /locus_tag="IV334_001423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291228.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glucose transporter GlcU"
     gene            complement(14885..15910)
                     /locus_tag="IV334_001424"
     CDS             complement(14885..15910)
                     /locus_tag="IV334_001424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAP8943501.1"
                     /translation="MNFFKYLKDQWTLILGWLFFIALTIFVMWLAPNIKVDLSTIAYL
                     ALIEGVFLIFYLLGGYLSKRRWWGKLADIQKTSPLQNYLSGGRTEEEKLVEEYINQLI
                     REHQEVMQSAISNQQDQKDYIDSWVHEIKVPLSASKLLVRSIEFDIDDQKYTLLENEL
                     SKIDEYVEQVLYYARLDSFSRDYLIQEYGLKGIIQPVIRSQANYFIQKNIRYEIVGED
                     HTVLTDAKWIAFIFNQLLSNAIKYTPDHGNIIVSIEKEAQGVSLSVKDSGIGIPAEDL
                     KRIFDKGFTGRNGRLSKTHSTGLGLYLAKNLAEKLGIHLTAESTEGKGTTMTLFFPIL
                     NFYNEKR"
     gene            complement(15928..16602)
                     /locus_tag="IV334_001425"
     CDS             complement(15928..16602)
                     /locus_tag="IV334_001425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010760322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP8943502.1"
                     /translation="MAKIMVVEDEEIIRQLIMEELEKWQFETFGTTDFNQVFSDFERE
                     EPQLVLLDINLPVLDGYYWCQKIREVSKVPIIFISSRNTNMDMIMAMNMGADDFVTKH
                     FQIDVLIAKINALLRRSYNYTELSSEMMSHNGITLNVDNGSMEINGEIIDLSKNEYRL
                     LFILMKHNGKILSREKLLRALWDDERFVDDNTLTVNINRLRRKIEQAGIQGYIETKVG
                     QGYIVP"
     gene            complement(16786..17679)
                     /locus_tag="IV334_001426"
     CDS             complement(16786..17679)
                     /locus_tag="IV334_001426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HAP8943503.1"
                     /translation="MKKKIGNRPQTQISLLLQLFMAVMFLAGAAVFTYPFLADALSNY
                     LDQRRIENYQKQLERDKEEKHEQQLAVHEKKNQTLAHTSVIPGMGQVKDPFEQAVRNV
                     QNPGKEYYEQHMIGAIYIPKINVSLPLFDETNDLLLDRGATVLQGTSFPTGGESTHSV
                     ITAHSGIAEKKLFTDLEEMEQDDRFYLEVYGRMLAYEVVEKIVVLPTKTNTLAIREKQ
                     DLVTLITCTPYTVNTHRLLVTGRRVPFTEEVSSKIEQTKKYHLYRLLILLLGILLVLT
                     LFGYWCYRKFKRQKQRKKNNR"
     gene            complement(17753..19450)
                     /locus_tag="IV334_001427"
     CDS             complement(17753..19450)
                     /locus_tag="IV334_001427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HAP8943504.1"
                     /translation="MNKKMIVTGMSVLLLVTAGIGIFGNGKLVKAEEVAQKPSEVTIT
                     LHKKGFSSVPEERPNSGLVSTDFGEENIPGVDFDLFDVTEVYYDLIRDNPLTPEREDG
                     LNSAEAIEWIQKRHTESWFLKYRLTSIDKQTTNEAGEAVFSTVQVTEEAPSSRDKVYL
                     FLETYSPAHISRIASPMVVMMPVMMPDMVDGVWDGSTWKDTYNTDVHLYPKNEIREAD
                     KQMNVEESDLRQVTIINEAGEQETISYIDLERGKTASYTITAPIPYFIDSVLENGSAV
                     IKNYKITDTPTVGLTYYDQEIEVRAGETILTKGQDYIVEVVSNGFVVTILTEENGVAK
                     VDTLGRLADARGGDLTITYNLKVSTELEADDFHNNTAVIEIGRNDEFDYEEGVEPPEK
                     VTTGGRKFEKYDASSSELLKDARFELWNEDRSEYAIFYKGESPLAVYESGADRIEWAT
                     SGQATEFVADGNGYFEVQGLDYGTYQMKETMAPEGYVLPTGEAAFTEFIISYGSYNEE
                     IQIVGVENPGPERVPNMKRGSLPATGGNGLLAFLLIGISLMIGAYSWYRKSKMKSEV"
BASE COUNT         5684 a         4190 c         3444 g         6158 t
ORIGIN      
        1 ttctttccct cttttttttg tttattcgaa ttctttttct tccagacgga ataacggact
       61 catcggtgta ttttcatgga tgcgtttgac cgcttcaccc attaattctg aacatgtaac
      121 gattgttaag ttttctggat gacgattttc ttctgtgaaa acagaatcag tgatacaaat
      181 atctttgatt ggtgcattgt ctaaaatact tttcgcatga tcggacaata atccatgtga
      241 cgcacaggca tatacttctt ttgcgccgtt ttcttttaat acatttgctg ctcgtgcaag
      301 cgttgctcct gtgttcaaga tatcatctac catgatacat gtctttcctt gaacatcacc
      361 gatcacataa ccaccctcag ttgaagcatc atctgcatga tctacgatgg ctaatgtgct
      421 gttcaaatat tctgaaagac tacgtgcacg ctgaacaccg ctgttttttg gcgaaacaac
      481 tacgatgtca tctcccgtca gacctaattg acggtaataa tgagcaaaca acggcatcgt
      541 aaataaatta tctacaggaa tatcaaagaa tccttgtact tgaaccgtgt gcagatctaa
      601 agtcagtact cgagttgccc ctgcttcttc gatcaagtta gcgatcaatt tcgcagtgat
      661 cggttcatga ggtttcgccg tgcggtcttg cctagcatac ccatagtaag gcaatacgac
      721 attgatcgtt ttcgcacttg ctcgcttcat tgcatcgatc atgatcagta gttccatata
      781 gtagtcattg acaggtgcat tcgttgattg aacgatatac acatgatccc ctcgaatact
      841 ttcttcgata ttgatggaaa tctcgccatc actaaattgc ttcacgacgc ttttacctaa
      901 ttctgttccg aacactttcg cgattttttc agctaatgga cggttgccat tcaaactaaa
      961 aatcttcaac gtgtcatcct gatacttatt actcatgtag tcctccccaa aacttctcta
     1021 ttcttcgata gtttaacata tttttccaag attttctgtt aatttcagca aattcttaag
     1081 gaagtgaaac tgttccgtta ctccaactgg caggaatgac tttatgataa tcgccccagt
     1141 tgtcgtgtac tttgtaataa cgttctcctg tttcagtttc aaaataagcg tacgctgtca
     1201 cccaatgatt gccatatgta ctgccaagta ctttcaaaat accaatcatc acaggtttgc
     1261 cttcacgtat acgttttgta gctcgctgcc atgaacctag aagagttgct cgtgcacgct
     1321 catgattgcc attcttcttt aaaaaactag aaatccctgt actaacctga aaaggaatcg
     1381 taggaagtcc tagtggttgg atctggctac gcaatgcctt cgtcaaaacc aacgaatcag
     1441 ccgtattttt cttgcgaatc tcatttggaa gcatccaccc cttacgatag tcttgataat
     1501 aggcaaggag aactgctgcc gcgtaagtac cacaaagata gcctgagggg gtagcccatt
     1561 tcttatacct caaaaaacgt tcttctggca atcccaccca gcaatttgcg agttcctttc
     1621 ctgacctttt ttcttcgatc aaaacgggaa tatcttcttt tctgttgatc catctagata
     1681 actcgattgg ctcttcattg atcagcttcc attttttaca agaaagaccc tcttgataag
     1741 tatattttgc tggagaatat tctaccggat gtcctgattg atcgataatc acataacttt
     1801 gatcttcaaa aaccagagct aaaaaaagca atttactttt tcttgactgc agctgctgag
     1861 tgctggctaa ttttttaatt tttttatcta ctttgaaata taaaggttta tccactactt
     1921 gattcagttg ggatcgcctc ctttttttca ttataagtga aggattgaaa aaaacaaatc
     1981 agtttgattg ttcgaatttt caatttttta tgttctattt gttaattcct tcattttgta
     2041 agataatggg cacacgaaac aaaaaaggag cgatcgatga atgaacaaag aacgattgaa
     2101 acaagatttc tatgaagcag tcaatgaaga atggcaaaaa acagcaaaaa tccctgcaga
     2161 taaaccagct acaggcggtt tccaagattt agttgacggg atcgaccaac ttttgatggg
     2221 agaaacagac gatctgcttg cccatccaga aaaagtgtct tcagaaaaaa tgaaacattt
     2281 ccttgcatat tatcaactgg caaacgatta tgaccgaaga aatgagttgc atgctcaacc
     2341 gcttcaacct ataatcgaaa aagttgaatc attgcagtct ttcgacgaat tgaataagca
     2401 atttccagaa tggctgttgg atacattacc attgccattt aatctggatg tagatgcaga
     2461 tatgaagaat gcgcaaacga acgccttctt tgcttatccg ccttctttat tccttccaga
     2521 taagacgtat tatgcatcag accatcccaa cggtgcacaa ctaataggcg ttttctttga
     2581 tatggctgtt cagttattag agcttgccgg caaagaaaaa agtcaggcag aagcaatcac
     2641 ggaacaagca gtgcaatttg ataaattgat tgctcctcat gtaaaaagtg cagaagaaaa
     2701 cgcagattac agcaaaatgt ataatccgca atcttttgat acatttatta aatacacaga
     2761 taaactggac ttgaaaaaac ttgtcacggg tttgattggg acaactccag ataaagtgat
     2821 cgtaactgat ccagtgtact tcgatcattt gaatgacatc ctgacagaag ctcattttcc
     2881 gttattcaaa agctggatga tcattaaaac agtacgttct cttagtggtt atctatcaga
     2941 agaattccgc caagtcagcg gaatctattc tcgcgtattg tctggaacag acgaagcaat
     3001 gcctcagaaa aaagcagcgt attaccttgc ctctggacaa ttcgatcaag tagttggcga
     3061 ttactacgga aaaaaatatt ttggcgaagc tgctaaaaaa gacgtagaac aaatggtgaa
     3121 aaaaatgatc gccgtttata aaaaacgtct agaagaaaat gactggcttg gggatgcaac
     3181 aaaagaaaaa gccgtcataa aattaaataa cttaggtatc caagtaggct accccgataa
     3241 gatcccagca ttattcgacc agttcaaaac agtacctgca gatcagggtg gtactttgct
     3301 ttcaaatact cttgttttca gcaaactcac attaaaagat cgtttcagca aatggaacaa
     3361 accagtggat cgttcagaat gggaaatgag tgctgacacg gtcaatgctt attatcatcc
     3421 atttcggaat gtgatcgtct ttccggcagc gatccttcaa gcaccatttt acagtctaga
     3481 acaatcaagc tctgctaatt atggtggaat cggagctgtt atcgctcatg agatttccca
     3541 tgcatttgat aataacggcg ctttatttga cgaatatggc aacttgaaca actggtggac
     3601 agaagaagat cttacacaat ttcaaaacaa agcacaagca atgatcgaag aattcgatgg
     3661 tctgcctttt gctgacggaa aagtaaacgg aaaattaacc gtttcggaaa atattgctga
     3721 tgcaggcggt ctaagctgtg cattggaagc agcaaaaacg gaatcagatc tttctatcga
     3781 ggaattcttt gtcaattggg caacgatttg gcgtacaaaa gcaaaaaaag aataccaaca
     3841 acttttactg caaatcgatg tccatgcccc tgctaaactc cgagcaaacg tgcaaccaaa
     3901 aaaccttgac gaattctatg aaacatttgg tatcacggaa caagacgcta tgtatctagc
     3961 gccagaaaaa agagtgcgaa tctggtaaga ggttgtgcca gaagtggtca gcttcaagaa
     4021 ataagaagga atttgggaaa atcgcttctc aaatttttgt gacatttcgg cttatttttc
     4081 gaagaagtta cttctggaat accatgtatt agaggttgtg aaaaagctgc ttagtttcaa
     4141 gtagtaagaa aaattttgtg aaaatggctt tccacatttt tgcataaatt tgacttacta
     4201 ccgagaaact ggcttttgaa caccgtttat cgagaggttg tgaaatcagc gtcctgacct
     4261 gagtattaga gaaaaaaacg gaaaaatagt tcctcatatt ttgccgtttg ttgagctaat
     4321 accgagggtc agctgattga acaccgttta gtggagagat tgtgaaaaag ctgtcctgca
     4381 tcaagtaata agaacagcca atcaaaagca gcaaggagaa cctcatctat aaaaagatag
     4441 aaggaagtga gacatctatt ggtctcattt ccttctattt ttactttcct cagtcaattg
     4501 ttttacatca taaaaggaga aattccactt atttttttcg ctgtttcatg acataaacaa
     4561 ctacaccgat taacaaaatt cctccagcta atgctagcaa cgggttatta aattctcccg
     4621 tttttgggag tccttgagtg ccattttttt cactttcttt tgctgtccta tccggatgag
     4681 aaggttttcc atttggtttt ccatttggtg attcatttcc tggattttct ctttctggtt
     4741 tgtcatttcc cgggggcaca tcggcagttt ctcgcacagt gatcgctttt tgctgggtaa
     4801 cagattctgt atgagcagac catttttccc catcaaatat ttccgaacga taagtgactt
     4861 ctagcgtata ttctcctttt gaaggtgctg aaaaaatagc ggtatgatta gaaaattgag
     4921 cacttgactg tgtttcaacg atttggatct tttctgggaa ataacgcata tcttcaaaga
     4981 caggcgttcc ttgatttggt gtgaaagttg acacaaaaac tgtaaaaggc ttcagttcaa
     5041 tgatatttcc ttctgcattc tccgccacac catttgcttc ttgtataata atcggattat
     5101 tttgttcatt tggttttgtc tgatttggtt cttgtacgat tgcatactct ggatattccg
     5161 aagacggtga ggtttctccg tcaaaaatag cacctacaga taatggaaga ccatccactc
     5221 tttccttttc tgtttgacga ttccccggtt taatctgcat cagtccatga acatggatac
     5281 ttccattttc atagcgcgta acaggctgct gaccttcttt cgaacatgta tttagagact
     5341 cgatccaatc ttttgatatg gtatgtccaa gagtattttc tgattgtttc tctcgttcat
     5401 tccaatagta gttcaacgag tggtagattt gatcttcgtt ttgattcaat gggcggatct
     5461 gctctctcat ctcaagattc tctgttcgga aagataagat accagaagca tggtagtctg
     5521 tgtttcctgc tgaagaggta taaagagcca cattatatga accgttatta aaacttgtac
     5581 tgctattgac gataatatct gttccgctat tggaatcgat tcctttcgca agattttcga
     5641 aagacaaact attgatcaat tcatgagggc ccgttaaaga agaaccgccc attttgaatc
     5701 cattaccgtt accttctccc tcaattcctg gtacccagcc gtttcgataa gcaactgagt
     5761 ttttgatgat tacttttccg atcgaacctg tttcattttt agcaaaaagg tcccaaccgt
     5821 catctgcatt atgatacgcg atacaaccgt caaatacatt tccttctcct attgtcagtt
     5881 tggccgcaaa tccatcggca tcttcaaatc ctggatcggc attttcaaaa gatgtacagt
     5941 tcttgactaa gttatgcgcg ggccatgctt cgaaaggatc atttgagcta ccgctgattt
     6001 ggattcctgt atttccattc cgataagcat gaatctcttc gatcaaatta tgatttccag
     6061 aaatctgtaa tcctttttgc atagctgcac tatttgtaat gtcaaaccct cgtaaacccc
     6121 agtagtgact gaacagtgtc atcccatttc cagttccttt aaaatcaagt acaggacgct
     6181 tgttttctgg atttttctct gcaatcaact gaatcgggtt ttctcttgtc ccgttaacgc
     6241 cttttggaat cgtcaatcct ctttcgaagg aataagttcc aggcgccatg acgattgtct
     6301 gcccagcata tgcgtatctc aatgcttgtg tcagagataa tggattttgt ctgcttccgt
     6361 ttcctgcatc tgacccttct ggtgttacat aaactgtcga atatttaggt ttacggtagt
     6421 ccactgtatg aagaatctct tttgtttcat aactagtcat ctctacgtat tcttctggcg
     6481 gatagttttt atctggcgta aaactgaaag taaattcgtt cgttccttcc atcagttttg
     6541 tctgataatc aaattcccga ttcgcagtga tagccaagtt ttttgcgttc ttaagcacct
     6601 ctcctgttgc tttattttta attgtcaata atccgtcagc atttgctcta aaggacatag
     6661 tatgatcttg gtttcctgaa tgattactag aggtcacagt gtaaatcgga tcaatcaagc
     6721 gagtaggtct ttcttgccca ggaggatctg tcgcaggatc tgagtaagca acttcgattc
     6781 cttctacctt gatcgtcata ttccttgctg cagcaaatcc tgcataaata accgattcat
     6841 ctgaagcgta taatttttcc cagtcataca tgatgtcaga agccatttct tctcctgttt
     6901 caggatcgta ataatatgac tctaaaccag tattcgtttt tttcatctct acgtccagtt
     6961 tagtaatcga gttcgtggcc ggaaacaaat tcccatgacc gtttccgaat atattataag
     7021 aacctgtttc tttttccaag aaccctgctg aagtctctaa aggtgttgtt tccactgaga
     7081 cactcccagg agctggcgca gcttgcggat cttcagatga tattcctgtg attgcccgtg
     7141 tccccaatcc aagtcgcatc gtatatttgc tgccgctcgt gttgaccact tcttgtctat
     7201 ccgagtccca gacatattcg atccgactgc ctagtatggc aaatgaattt gaataaaatg
     7261 cctgcccaaa caaatgattt tttggtactg cgtctcttac cattaaagca aatccttctt
     7321 gggcattcgt ataggtccaa gattctattt cgatcgtcgc ccgcatccta aagttgatgt
     7381 ctttggggat tgcttgataa taataggtca ttccatctga tccgcttgac tggaattttc
     7441 cgccgttatt cgtggaagag agccgcacgc cgtcttctaa tggttcaatg gcatacctac
     7501 tagcacttgt gctttctccg aaaacaaccc ctttccactc ttcatttgct tcttcagatt
     7561 gcttttttgt cttttcattt tctgattctt tcatcatagg atcatttcct tcttccgtat
     7621 ccatatcttc actagttctt ggtacttcct ccattgttga catcccttca atgttcacgt
     7681 gtgcattttc ttcgttcaca gactgttgtt cgttagcagc aacttcaacg acagaaatta
     7741 aaaccggggt actgagcata aggagattca tcattagcgc actgtatttt atacgttttt
     7801 ttaacaagtg tattcctccc gtaacaaatt agaaagcgtt tacttaaagt attacctaat
     7861 ttatttttac tgtcaataaa aaacaaaatt ataataagtg tattttataa aaagaaaaaa
     7921 tataacttaa gttgttgata ataaagagat ttattaacgt aaaaagaata agttttttta
     7981 aaataacgta taaaaaagag cataaaacaa agtcgtttgt tttatgctct tcttttatta
     8041 ttttgtgctt tttattttat caaatcactt tgttttttta taaaatttct gcgcctagtg
     8101 tggactcaaa atgtctcaat gcccattcat gtccaacggg atcgaaactt gccacggcat
     8161 ctttgaaaat atggagtttg tatcctagat tatacgcatc gactgctgta tgcaacacac
     8221 agatatctgt acatacgccg gtcaaataaa tatctgtgat ctggcgttct cgcaaacgga
     8281 tgtctagatc tgttccacta aaagcggaat aatgccgttt gtctatccaa taaacatttt
     8341 cctcttgctc atagtcttgg tataaacttt ttaaagagcc gtacaattcc cgtccacccg
     8401 ttcctatcac attatgcgga ggaaataagc ggttctccgg atgaaaggaa tcttttggat
     8461 catgcgcatc aatggcaaaa acgacaaaat ccttttgatc aataaacttc tttgtatgag
     8521 cagtcagctt tttttcaatt acttgtcctg ctgtcccagt agtcagcttt ccatcctctg
     8581 caacaaaatc atatgtgtag tcaatcgaga tcaatgcgtt catcttagtc ctccttatac
     8641 gagatgtttg acaaattcga tcaccatttc tgctgatcgt ttgcctgcgt cttcgataaa
     8701 ttcgtcaaag ctttgtgtcg ccgagtgatc tgctgtgtcg ctcattgcac gtacgatcaa
     8761 aaatgggata ttgaactgac gagctgtttg tccaacagcg gctccttcca tttcgcaagc
     8821 taatgcttca gggaaattcg ttaagatttc tttgatttta tctggcgagt cgacaaatgt
     8881 atcacctgta acgatcaatc cttcttttgc gttcaaattc gttgcttttg ctgctttaac
     8941 catttcactg cgcaaatatg tgcttgcttc ataatataga ggcattcctg gtaattgtcc
     9001 tggtttgtag ccaaatcctg ttgcatctgc atcaaaataa gctactttgt ctgatataac
     9061 gatgtcacct acttgtaagc cttcaccgat tccgccagct gaacctgtat tgatcaccat
     9121 gttcacgccg tattgttgga tcaacagact tgtggtaata gatgctaaga cttttcctat
     9181 accggaacgt acgacgatca cttcgtggtt acctaatgaa cctgaaataa ataaagcgcc
     9241 tgcgcgttcc catgataatg gttcgcttag gttctctctc aaaattttta cttcttcttc
     9301 cattgcgccg ataataccga ttttcatctt tttaattcct tctttctata taaatgcgat
     9361 tgctaaaaaa accacacata acattaggat aacaataata agagatttca tcaaaaaaga
     9421 gttccatttg gtcgtctttt cccgttcgct gatccttgtt ttcgtgattg gtttattctt
     9481 tttgctttct ttacgataaa agctggcaat ctttttttct tcttgctgat aagctgtttc
     9541 tgcttttcgc tgttttttta atgactcgct tgcttgtgct tgttgtcgct tgcgaagttc
     9601 gcttcgcgta ataagcggtc ctttggccat aagccgatcc ctctactttc ctttggtcaa
     9661 tagttcccag tattgaatcg cataaatcgt ctttgcatca caaatcaact gatctttcat
     9721 cgcttgttgc gcctcttcaa gagttagatg atacaactcc agtacttcgt cttcatcttg
     9781 tgctaaagga ttctcgacct tctctacccc ttgtgcatga taaatatgaa ggacttcatt
     9841 tgcaaaacct ggtgaaagat acatcgaagt aagatggctc agcgacttcg cacgatagcc
     9901 agtttcttct tctaattctc tagctgctgt catttcggga ttttgtcctt ctcctggatc
     9961 gatttttcca gctggaatct ccaaaatcac tttttctaaa ggttttcgaa attgtttgac
    10021 aagaagcaga cgatcttgtt cgtcaaaagc aatgatcccg acaccaccag gatgaaatac
    10081 cagttctctc ttcgaagttc ctccatctgg caaacgaact tcgtctacag caacatcaat
    10141 aatttttccg ctatagattt ctttgcgact gattgttttt tcttcaaatt gctcattgtt
    10201 cagcatatct tctcctgctt tctttgtaat cataactgct tttttttcat ttttcaattt
    10261 ttctcaaaaa ctccttatta gtttaccata gataaacctt agattttctc taaatataga
    10321 aaatccttct ttagtccgat actctttcta gtagtccgta tgctaaaata agtatgtaat
    10381 cgaataacga aaagaattca atactcaacg ataaatgaca tggagggttt attatgataa
    10441 aacgaaattg gccgtggctt ctagcattgg tcttgctatt tctctttgtc cgaaacaata
    10501 gtttttcttt tgttctagga cttttattga taggtgtagt tttagttttg tggggactct
    10561 ttggttcgcg caggaaaaag ggaaaacaag aagaaattcc tgcactgact gacgaacttg
    10621 aaactcacta tgaaaaaagc ggtatgacag cgaatgaaat ttcatttttt cgacaaacaa
    10681 tgaatcaaac aaaaaatgag atcaatcaat tgcaaatgaa tatgcagcag acagccaaac
    10741 ttaaagcaat cgatctccgc catgatccag tcaaagcagc gaaagcttta ttcaaagaat
    10801 tggtgaaaga accaaaccga ctgcatgaag caagtcagtt tctttatacg catctgccga
    10861 atctagttga tttgacaaat aagtataatg agatcaatga ccatgagatc aaaaacaaac
    10921 agacctatga aaaactagaa gaaagcgcgc agatcatcga ccagctgtca gctttaatcg
    10981 cacaagatta tcaaaaattc gttgctgagg atctagatga tatcgacgta gagatatctg
    11041 ttgcgaaaca gagcttaaag cgtgataata aatatgaacc agaacaaaag gattaactct
    11101 tttgttcata aaagaagact cggaacattc agatgatgtc tggctgtttc gagtactttt
    11161 tctaaagtag agacattcat gtaagaatcg agaacagatt caacaaggag gaatttttta
    11221 tggatcataa accgactgaa acaaaagatg ttacaccagt taacgataca cttgaagatt
    11281 tattgaacaa cccgttttca actccaacag atacgctgac cacttcacaa caagcggaga
    11341 ttgatcagct ccaagagcaa cagacagctg ctcgtttaat tgacaaacta ccggcggaac
    11401 gccaagaaca agctaaacag ctagcagcaa aaatcgatgc taatgatgca cagtctgtca
    11461 tcagctacgg atcggctgcc caagcaaaat taagtgaatt ctcacaatcg atgttgaatc
    11521 atgtgcaagc acaagatatt ggtcctgtag gagattcctt aactgaactg atgtatcgtc
    11581 tccaagaagc caatcctgac gaactgcgtg ccggagaagg gaacatcttc cagcgtgtct
    11641 tcgggaaggt caaacaatcg atctatgaag tgacagcaaa atatcaaaag attggtgcac
    11701 aaattgacaa gatttcagtg aaattagata aagaaaaaga cggtctgcta aaagataatc
    11761 tgatgttaga acagctttac cagaaaaaca aagactactt tgatgcactg aatatttata
    11821 tcgctgctgg agaattgaaa atggaagaac ttcaaacaac gatcattcca gaagcgatga
    11881 aaagagcaga agaaactggc gatcagatgg atgttcagat tgccaatgat tacacgcaat
    11941 tcctagatcg tctggataaa cgtacgcatg atcttcgctt agctagacag atcacgatcc
    12001 agcaagcacc acaaatccgt ttgatccaaa acacaaatca ggccttggct gaaaagatcc
    12061 aagcatctat cgctacagcc attccgctgt ggaaaaacca agtcgtgatc gcattgacct
    12121 tgcttcgtca aaaagatgca gtaactgctc aacgccaagt ctctgaaaca acaaacgact
    12181 tattgaagaa aaattcagaa atgctgaaaa tctcagccat cgaaacagcg aaagaaaatg
    12241 aacgtggaat cgtggatatc gagacccttc aaaccactca aaatgatttg atcgaaacaa
    12301 tccaagagac attgcgtatc caaaaagaag gaaaagaaaa acgtcgtcat gcagaagtag
    12361 agcttggaca catggaagaa gatctgaaac aaaaactgtt ggatttaacc caatagatca
    12421 cctttcgctt gttactaaaa caaacgccca acaacagttt attacaactg ttgatgggcg
    12481 tttttcttat ctttcttcta acttctccat tggagtctat ttctcattca tcttgctgtg
    12541 tccatagcca tagccaaaat aaatgatcag tccaataacc attacgatac cgaacacgat
    12601 ccatgtcaca gtttgcagac gaagcatcaa tacaatacaa gtcaagatgg agacgattgg
    12661 caatagcgga acgaatggca ctttgaattc tccagctttt ggtttgccaa acatttttct
    12721 caacttcagt aagcctagac taacaagcat caaataggcg agcgccatga tattcgttag
    12781 ttccgctaat acattcaatg ggaaaaatcc ggcaaagaat acagctgcca caccagcggc
    12841 ataagtcgct tttttcggtg tacgatgctt ttgatcgatt tctgtcataa acttaggaag
    12901 taagccatcc ttgctgacag caaacagtaa acgagcaagt gaatacatca tcgagatcgt
    12961 taccgtcagt agcgttaaaa ttgcccctac agcgatcacc gcgcccactg ttccatgtcc
    13021 tacatacctc attgcaaaag ccactgggtc attgactcct agttcagtat acggaacgat
    13081 tcctgtcaag atcagcgtca cgatgatgta aaggacagta gcaatcagta tcgaaccaat
    13141 gattcctctt ggcacatctt tttgcgggtt tttgacttcc tcggctgcca ttgcaacggc
    13201 atcaaatccc aagaaagcaa aaaacacaag agctgcacca tctagcacac cggaaaaacc
    13261 aaaaggcatg aatggctgcc agttatcagg tttaacgaaa aatattccta caatgataaa
    13321 caacgcgatg ataccaaatt tgacatatac catggcatta ttcaaacgta atgctttctt
    13381 ggcttcaagc gacacgatgt aagtgacgaa tatcactacg agcaccgcaa tcaagtcaat
    13441 gtatgtacca ttttccggat tgtacccgcc agttaatgct tgtggaagtt cggcccctaa
    13501 acttttcaaa aaaccttgca tatagccaga ccatccggaa gcaacagagg aaaccgctag
    13561 taaaaattcc ccgatcaaca accaccctgt cagccaggca gcaaattcac caaatacaac
    13621 atacagatag gcatatggcc caccaatcac tggcactcgc gaagcaaatt ctgcgaaact
    13681 tagtccagac aaaatgacaa cgattgcagc taagacaaat gaaagtgaca gtgctggacc
    13741 agcattttga ttggccgcaa cccctgtcac cacaaatatt cccgtaccga tgatcgcacc
    13801 aattccaagc atgatcaaat cagacgtttt tagttctttt ttcatctcac tcgtatgatt
    13861 gagattgatc tctttcttac gaaacaaatc cattgaagtt cctccccatt ttaggttgga
    13921 aaacagcgat taaactgttt tcatataccc taaaaggata ccacctaaaa tgactaaaag
    13981 gcaaccaaca ataacataaa tgagctcttt tttcgttttt ctttcgccaa ggatgaaaat
    14041 cccacctaaa gtagagataa tgatccccat ttgtgaaagt gagaagccaa cagctaaacc
    14101 aagtgatttg actgttagaa gcatgcagat gtttcctaag ccccataata atccacagat
    14161 catgtttttc cacacaaact gatcgacttt aactttacgg aaagcaaaga agcttgcgcc
    14221 taagatcatt ccgatacttt gaggtaagat aattgacatc gcatccaaat tcgcccagtt
    14281 gacgatgatg gtatatacac cataaccaat cgtagagaag atcaatgctc taaaaccctt
    14341 accaaaatca agcattttat tatctgtatc tatttttcct tcgtcatctt gtcgggcagt
    14401 caaatacgca ccgcttacaa gaagagctaa agcaataata ccctatacat aatctttcgt
    14461 ttgtgtccat tcatggaaga aaacggctcc cgcaatcgta ttcaaaatta attgaaaacc
    14521 agtagacaat ggtagcccta cagaaacacc catgtacttc attccatgga actgtccatt
    14581 ttgtccaaca ctccaagcta agccggataa aaatccgatc aagaaaatcc aaggggtgat
    14641 tacaggacta acaacgaaaa agacaactaa agaaaacaga aatgcaccga tcgtcattcc
    14701 tagtgtttgt tgattggcat cgccaccaat tttaccactt actaaaccaa tactacccca
    14761 tgccaacata gggactaatg ctattaaaat ttccataacc aatctccttt tatttcacat
    14821 gataagaaac ttatcagcga aaacgttaac aacacaagca ctttttttcg tttctaacga
    14881 ttttctaacg tttttcatta taaaaattta agattgggaa aaataaagtc attgttgtcc
    14941 cttttccttc agttgattca gccgtcaaat ggatacctaa tttttcggcc agattttttg
    15001 ctaaataaag tcctaatcct gttgaatgtg ttttgctaag tcgaccattt ctcccagtaa
    15061 atcccttatc aaagatccgt ttcaagtctt ccgctgggat accgatccct gaatctttaa
    15121 ctgaaaggct gactccttgc gcttcctttt ctatgctcac aatgatgttt ccgtgatccg
    15181 gcgtatattt gatggcatta ctaagcaatt ggttgaagat aaaagcgatc catttagcat
    15241 cggtcaaaac agtatgatcc tcaccaacga tctcgtagcg aatatttttt tggataaaat
    15301 aatttgcctg actgcgtatc acaggctgaa tgattccttt tagtccatat tcttggatga
    15361 ggtagtcccg cgagaaactg tcgagtcttg cataatacaa aacttgttct acgtattcat
    15421 cgatttttga taattcattt tctagtaatg tatacttttg atcgtcaata tcgaactcga
    15481 tcgaacgaac tagcaattta gaagcggaaa gtggcacttt gatttcatgg acccatgagt
    15541 caatataatc tttttggtct tgttgattac tgattgctga ttgcattact tcttggtgtt
    15601 cacggatcag ctgattgata tattcttcta ccagtttttc ttcttctgtc ctcccaccac
    15661 tcaaataatt ttgcaaaggg gaagtttttt gtatgtcggc taattttccc caccatcttc
    15721 tcttgctcaa gtaaccaccc agcagataaa aaatcagaaa gacgccctca atcaaagcta
    15781 aataagcaat tgtgctcaaa tcaactttga tgtttggcgc cagccacatc acaaagatcg
    15841 tcaatgcaat aaaaaacagc catcccaaga tcaatgtcca ctgatctttc aaatatttaa
    15901 aaaaattcat cttaaccgct catcctttca gggaacgata tatccctgtc ctacttttgt
    15961 ttcgatatat ccttggatac ctgcctgttc gatttttcgg cgtaaccgat tgatgttgac
    16021 tgtcaatgta ttgtcatcta caaatcgttc gtcatcccac agtgctcgca gcaatttttc
    16081 ccgacttaag atctttccat tgtgcttcat caaaataaat aatagacgat attcattttt
    16141 gctcaaatca atgatctctc cgttgatttc catacttccg ttgtcaacat tcaacgtaat
    16201 gccattgtgg ctcatcattt cactagataa ttctgtgtag ttgtaagaac ggcgaagtaa
    16261 tgcattgatt tttgcaatca gtacatcgat ttggaaatgt tttgtcacaa aatcatctgc
    16321 ccccatgttc atagccatga tcatatccat attcgtattc ctgctggaga taaaaatgat
    16381 cggtactttc gatacttcac gtattttttg acaccaataa tagccatcca aaacgggaag
    16441 attaatatct agtaatacaa gctgtggttc ttctctttca aagtctgaga acacttgatt
    16501 gaaatcggtc gttccaaagg tttcaaattg ccatttctca agttcctcca tgatcaattg
    16561 tcgaatgatc tcttcgtctt cgactaccat gatttttgcc ataaaaaatc cctccatctt
    16621 tcttcttgct actacaatat caaagaagcc cttgtgaaac aagttggctt acctggaaaa
    16681 tgtcatttta gcaaacgtta aaaaaaactg gaaatatgcg atttaatcgc gtttgtctct
    16741 tccaagcagc ttaaatttat tatgttattc ttaataattg gtctattatc tgttattttt
    16801 ctttcgttgt ttttgtcttt taaattttcg ataacaccaa taaccgaaaa gcgttagcac
    16861 tagcaatata cctagaagga gtataagtaa tcgataaaga tggtattttt ttgtctgttc
    16921 tatctttgaa gagacttctt ctgtaaaggg aactcgtctt cctgtcacta gtaatcggtg
    16981 agtgttcacg gtgtaaggtg tacaagtgat aagtgtgact agatcctgct tttctctaat
    17041 tgctaatgta tttgtttttg ttggaaggac aacaattttc tcaacgactt cataagcgag
    17101 catccgacca tacacttcaa gatagaatcg gtcatcttgc tccatttctt caagatccgt
    17161 aaagagtttt ttctcagcaa taccgctatg ggcggtaatc acggaatgtg tactctctcc
    17221 tccagttgga aaagaggtgc cttgcaggac tgtcgctcct ctatctaaca aaagatcatt
    17281 cgtttcatca aataaaggca agctaacatt gattttcgga atatagatgg ctccgatcat
    17341 atgttgttcg taatattctt ttcctggatt ttggacattt ctaaccgctt gctcaaaagg
    17401 atctttgact tgtcccattc ctgggattac tgatgtatga gcaagcgtct gatttttctt
    17461 ttcatgcact gctaattgtt gctcatgttt ttcttcttta tcgcgttcaa gctgcttttg
    17521 ataattctcg atccttcttt gatccaaata attgctgaga gcatctgcta aaaatggata
    17581 tgtaaaaact gcagctccgg caagaaacat cactgccata aataattgta ataaaagact
    17641 gatttgggtt tgaggtctat tacctatttt ctttttcatt ttcttcacct tcttatttaa
    17701 aaaaactgca gcaaccttta taaaaccgat tgctgcagct tatttttgtt gcttatactt
    17761 cagacttcat tttagattta cggtaccagc tgtacgctcc aatcatcaaa cttattccga
    17821 ttagtaagaa tgccaagagc ccgttcccac cagtagcagg aagtgacccg cgtttcatat
    17881 tcggtactct ttctggccct ggattttcta ctcctacgat ctgaatttct tcattatagc
    17941 ttccataaga gatgataaat tcagtaaagg ctgcctcacc tgttggaagt acataacctt
    18001 ctggcgccat ggtctctttc atttgatagg ttccataatc taacccttga acctcaaagt
    18061 atccatttcc atctgcgaca aattctgttg cttgtccgct tgttgcccac tcaattctat
    18121 ctgctcctga ttcataaaca gctaaaggcg actccccttt ataaaatatt gcgtattcag
    18181 aacggtcttc attccacaat tcgaatcttg catctttaag caattcactg cttgatgcat
    18241 catatttttc aaactttctt ccacctgttg tgactttctc tggcggctct actccttctt
    18301 cataatcaaa ttcatcattt cgtccgattt caataaccgc tgtgttgtta tggaaatcat
    18361 ccgcttctag ttctgtggaa accttcaaat tatacgtgat cgttaaatct cctccacgtg
    18421 catctgctaa tctacctaaa gtatctactt tggctactcc attttcttct gtcaaaatcg
    18481 tcacgacaaa cccgttgctt actacttcta ctatataatc ttgtcctttt gttaaaatcg
    18541 tctcgccggc tcgcacttct atttcctgat cataataagt tagaccaact gtaggcgtat
    18601 ctgtgatttt gtaatttttg ataacagcgc ttccgttttc aaggacagaa tcgatgaaat
    18661 aaggaatagg tgctgtgatc gtgtaagaag ccgtttttcc tctttctaga tcaatataag
    18721 aaatcgtttc ttgttctcca gcttcattaa taatcgttac ttgtctaagg tcgctttcct
    18781 ctacattcat ctgtttatct gcttctctga tttcgttttt cggatacaaa tgcacatctg
    18841 tattgtatgt gtctttccat gtagaaccat cccacacgcc atccaccata tcaggcatca
    18901 tcactggcat cataacaacc attggagacg cgattctgga aatatgagct ggcgaataag
    18961 tttctaagaa aagatagact ttatctctag aagaaggtgc ttcttccgtc acttggacgg
    19021 tagagaatac cgcttctcca gcttcattcg ttgtttgttt gtcaatactt gtcagtcgat
    19081 attttaaaaa ccaactttct gtgtgccgtt tttgaatcca ttcaatcgct tcggcagaat
    19141 taagaccgtc ttctcgttcc ggtgttaaag gattatctcg aatcaaatcg taatatactt
    19201 ccgttacgtc aaacaaatca aaatcgactc ctggaatatt ttcttcacca aaatccgtag
    19261 aaaccaatcc gctattcggt cgttcttccg gaacagatga gaaacctttt ttgtgtaatg
    19321 taatcgtcac ttcacttggt ttttgtgcga cttcttcggc tttgactagt tttccgttcc
    19381 cgaaaatccc tattcccgca gtaacgagta acagtacact cattcccgtg acaatcattt
    19441 ttttattcat ttttttctcc ccgttctttt tttatt
//
LOCUS       DACDKM010000042        19299 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20834_2016
            SAMN12640370-rid9891663.denovo.042, whole genome shotgun sequence.
ACCESSION   DACDKM010000042 DACDKM010000000
VERSION     DACDKM010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640370
            Sequence Read Archive: SRR10040891
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19299)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19299)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742835.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742835.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:04:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,012
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 29 of 75
            Pseudo Genes (incomplete)         :: 33 of 75
            Pseudo Genes (internal stop)      :: 19 of 75
            Pseudo Genes (multiple problems)  :: 5 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19299
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640370-rid9891663.denovo.042"
                     /isolate="E20834_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            64..849
                     /locus_tag="IV334_001428"
     CDS             64..849
                     /locus_tag="IV334_001428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SinI family restriction endonuclease"
                     /protein_id="HAP8943505.1"
                     /translation="MPNKNRTFESILNEGLTTEPASIIFDEVIDDVEDSKYHKNLKEL
                     FYFALNNGPSLFTGISVPDSDNPLIMAKIYLTKWCNKYIRDRNNPALKKPLKTFGEKD
                     AALTTRVAANANIDDQKVLNDYLRGHFLYMSAENMNGSILEEYLAEVLEPEGWIWCAG
                     SVYRAVDFCYLGTSPILLQVKNKYNTESSSSSAIRVGTTIRKWNRLNKSTKISGLDSP
                     IPNWKALIEMTEASKELAAKLTENSYLAYINEKSTRELWTLDD"
     gene            1558..1917
                     /locus_tag="IV334_001429"
                     /pseudo
     CDS             1558..1917
                     /locus_tag="IV334_001429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611166.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            complement(2212..3345)
                     /locus_tag="IV334_001430"
     CDS             complement(2212..3345)
                     /locus_tag="IV334_001430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HAP8943506.1"
                     /translation="MLLNFKMTLTMLAFSLLVTGVILVSFFFLRNNLEGISIASTLGE
                     DVYFMEEGKTVQAETQMEASKSSNNQFMTISMIDKAYQDELFKYLPAMVLVICSAVML
                     LTFILWLVLRKIYSNQILAITRDLTFIDQVPKNTVKDEALNAAFNKLKDKFDGHLEDY
                     RRLNSYLTHEQKNAIAILRTNLEIERQHADQGNLMLLDRMTDSIDDILTLSNNSEDLS
                     TEVVDVSLICAEVCDAYKMNYPNLLFAFTEDQPTLIHGRDRWIYRAVSNLVDNAIKYG
                     EGKLVIVTISNQKGSVIIEVKDQGIGIPQEKQASIFSHQYRVHGLKQDGLPKTMDFRD
                     TDETFIQSVASKRNHIPRKSLNYRTPLEVFLSCIDKDILSSLI"
     gene            complement(3336..4004)
                     /locus_tag="IV334_001431"
     CDS             complement(3336..4004)
                     /locus_tag="IV334_001431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP8943507.1"
                     /translation="MRVLVVEDNRELVLSVKKGLEQENISVDVAYDGESGEEKAFINS
                     YDGILLDLNLPKKDGLSVLQSLRAEEVESPVIIITARDEIEERAKGLDFGADDYLVKP
                     FELLELKARIRAVIRRYHGRALPQISIGALKIDPLGRRVSYQKTEVVLKPKEFDILLC
                     IAQRHPAVVSSEEISEHVYDEDFDPFSSVLRVHLARLKKKLAEAAEKEVLKTIRAKGY
                     QLCY"
     gene            complement(4460..5446)
                     /gene="lacD"
                     /locus_tag="IV334_001432"
     CDS             complement(4460..5446)
                     /gene="lacD"
                     /locus_tag="IV334_001432"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016178524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase"
                     /protein_id="HAP8943508.1"
                     /translation="MNEEKRQYIKQLCNEDGVIGALAIDQRGALKKMITKFKGSEAEE
                     SEIVEFKEIVSSQLTKYASSILLDPEYGLPAADVRAKNAGLLLAYEKTGYDASTPGRL
                     PDLLSIWSVKRLKEAGADACKFLLYYDVDEAEEINNQKHAFIERIGSECQAENLPFFL
                     ELVSYDANISDATSKEYAIKKPHKVNDMMKEFSDPRYGVDVLKVEVPVNMNYVEGYGT
                     DEVVYTKAQAANFFKEQAEATNLPFIFLSAGVKAELFQETLRFAKNAGSTFNGVLCGR
                     ATWADGVESYIVDGKEAAMEWMNTQGRKNIEELNAVLKETATPVEVLTSSLS"
     gene            complement(5459..6385)
                     /gene="lacC"
                     /locus_tag="IV334_001433"
     CDS             complement(5459..6385)
                     /gene="lacC"
                     /locus_tag="IV334_001433"
                     /EC_number="2.7.1.144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-6-phosphate kinase"
                     /protein_id="HAP8943509.1"
                     /translation="MILTITMNPSIDISYPLETFKLDTVNRVRNVRKTAGGKGLNVTR
                     ILKQLDTEVIASGLIGGFLGEEIKKDLTQNEIAHDFLPISGETRNCIAVLHDGKQTEI
                     LENGPVITEQEARAFLQHFEKLAVKAEIISFSGSLPEGLPADFYRQMLQVCQPKPVVL
                     DCSGTALREVLRGSVKPKLIKPNTEELAGLLGREISTELSDLKAALSDSMFDEIEWIV
                     VSMGGDGAFAKHHETFYCVTIPKIDVVNPVGSGDATVAGLTAAIQQGLSDEAVLKQGN
                     VLGMLNAQEATTGYVQLANYQKLYDQILVEKV"
     gene            complement(6398..6913)
                     /gene="lacB"
                     /locus_tag="IV334_001434"
     CDS             complement(6398..6913)
                     /gene="lacB"
                     /locus_tag="IV334_001434"
                     /EC_number="5.3.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-6-phosphate isomerase subunit LacB"
                     /protein_id="HAP8943510.1"
                     /translation="MKIAIGCDHIVTDTKMAVSDFLKSKGHEVLDMGTYDFTRTHYPI
                     YGKKVGEAVTSGEADLGVCLCGTGVGINNAVNKVPGIRSALVRDMTTAIYAKEQLNAN
                     VIGFGARITGEFLICDILEAFINADYQETEENKKLIAKIDHVEKASDKQSDPHFFDEF
                     IEKWDRGEYHD"
     gene            complement(6932..7360)
                     /gene="lacA"
                     /locus_tag="IV334_001435"
     CDS             complement(6932..7360)
                     /gene="lacA"
                     /locus_tag="IV334_001435"
                     /EC_number="5.3.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010780421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-6-phosphate isomerase subunit LacA"
                     /protein_id="HAP8943511.1"
                     /translation="MRVVIGSDSDGMELKELIKKKLIKEGITVVDKSETASEDFVEST
                     LAIANDLNENEGSLGIAVDGYGAGSFITATKVKGMVAAEVSDERSAYMTRKHNNSRMI
                     TLGSKIVGDELALNIVKEFLQANYDGGRHQVRVDMLNKMA"
     gene            complement(7380..8153)
                     /locus_tag="IV334_001436"
     CDS             complement(7380..8153)
                     /locus_tag="IV334_001436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010731480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HAP8943512.1"
                     /translation="MLKTERQRSILAMCDRYGTITVKFIQEVLNVSDMTIRRDLEELA
                     QKKKLLRVHGGAQKIDYLEMSTSIEANELSHTEKKKLHTKEKEYIAKNAAAHINQEDD
                     TIFLGSGTTIELMTRYIKPSSLRIVTNSLPVFNLLSEIKRFELYLIGGLYREKTGAFV
                     GSIANEAVQKIGIKKAFVGVNGLKGSEVSNFSIEEGTLQKIVLDNAAEKCLVSDANKF
                     DHSDFYNFYDLRKIDKLFTDSTLSAELKSYYQQFTKIIQ"
     gene            8318..8797
                     /locus_tag="IV334_001437"
     CDS             8318..8797
                     /locus_tag="IV334_001437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016178527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAP8943513.1"
                     /translation="MNEFFFKDTVYVSDKQSQEEVFEEVYQDLLKKELVTEDFLENLL
                     ERERNYPTGLDLSPVSKILPDIAIPHTESEFVRTTRIIPIKVKNEIPFYNMISPSDQI
                     SASFLFMILNENGEAQAGMLANIMDFINSVDEEKLFSFFKLEDTEAIYHFLEEHFYI"
     gene            8854..9156
                     /locus_tag="IV334_001438"
     CDS             8854..9156
                     /locus_tag="IV334_001438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003088862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIB"
                     /protein_id="HAP8943514.1"
                     /translation="MIKILAACGAGVNSSHQIKSALETELKNRGYQVTCDAVMIKDIN
                     EEMLSHYDIFAQIANTDLGFDINIPVVDAGAILYRIPAMAEPVYQSMESAIQSLNK"
     gene            9178..10635
                     /locus_tag="IV334_001439"
     CDS             9178..10635
                     /locus_tag="IV334_001439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol transporter subunit IIA"
                     /protein_id="HAP8943515.1"
                     /translation="MNTIIDIANKVFTPLINLGAAPLMTIVLTVIALFFKVKPSRALE
                     GGLKLGIALTGIGAIIGILTTAFSDAMTAFVERTGISLNITDVGWAPLATITWGSPYT
                     LYFLLIMVIVNIVMLAMKKTNTLDVDIFDIWHLAIVGLFAIFCGANLFIASALVIFIG
                     VLKIWNSDLMKPTFNDLLNAPDSNPMTTTHMNYMMNPIIMVFDKIFDKIFPWLDKYDF
                     DAAKLNSKIGFWGSKFAIGIYLGIFVGLLAGQTPTQIFSLAFTAAVCLELFSLIGAWF
                     IAAVEPLSQGITDFANKRLKGRTINIGLDWPFIAGRAEIWAAANVLAPIMLLEAIILP
                     GNKLLPLGGIIAMGVTPALLVVTRGKLIRMIVIGAIELPLFLWSGTLIAPFVTQTAKA
                     VGAFPAGLNGSAMISHTTMEGPIEKFLGYLVGNASQGQIEFVIYAALALGAYLLIFIW
                     YARQMKKRNAAYAAEKEENASPATAHNMTYAAEAK"
     gene            complement(11228..11980)
                     /locus_tag="IV334_001440"
     CDS             complement(11228..11980)
                     /locus_tag="IV334_001440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HAP8943516.1"
                     /translation="MLKTDRFQAIMSLLEVNGTVRVSDIMEKFSVSDMTVRRDLDEME
                     KQGLLERVHGGAKLKDFYRHVELSHREKQIMLQEEKQRIVEKANEMINDGDTIFLGPG
                     TTIELMSKIIQGDSLRIVTNCLPVFEELNKRKGNRKIYLLGGEMRETTQCFVGEITNK
                     SLQDMNFHKAFFSCNALNEQKIMTSTFEEGQTQQIALNNSVERFLLIDHSKIGKRDFS
                     VYYNLNDVTVVITDDDEQKHYEKIEKEEKYVV"
     gene            complement(12195..12431)
                     /locus_tag="IV334_001441"
                     /pseudo
     CDS             complement(12195..12431)
                     /locus_tag="IV334_001441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016178530.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UTRA domain-containing protein"
     gene            complement(12441..13399)
                     /locus_tag="IV334_001442"
                     /pseudo
     CDS             complement(12441..13399)
                     /locus_tag="IV334_001442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
     gene            complement(13605..15287)
                     /locus_tag="IV334_001443"
     CDS             complement(13605..15287)
                     /locus_tag="IV334_001443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP8943517.1"
                     /translation="MNTIIKQIEKGKPFFEKISRNIYLGAIRDGFLTAMPAILFSSIF
                     IMIAAVPEVFNVTLPEAFSTWLWKVYGYSMGLVGLLVVATTARCLANSMNRQLSDSGK
                     QINEVSVMLASICGFVMMAADQIDGGIATDYLGTKGLLAAFVSAFVTVNVYKFCVKRD
                     LTIRMPKEVPGTITQMFRDIIPFSIAVFIAVLIDTGVRQFFGTSFAAGVIQLLQPLFS
                     AADGYVGIAIIWGAMAMFWFVGVHGPSIVEPAIAAIIYANVETNFELFKNGAQASKVL
                     TVGLGNFVGTMGGTGATLVVPFLFMLFAKSKQLKAVGKASFIPVSFAVNEPLLFATPI
                     ILNPYFLIPFLLAPIANVWIFKLFVDVLGMNSFISVLPWATPAPIGLVLGTGFSVLAI
                     VLAVVLIVVDAIIYLPFVKAYDSELVEQELKNIAAEEVKEELVEEIQEVGATAAVIVD
                     EAKASVGEGKRVLVLCAGAGTSAMLANALEEGAKETGVNLSAKAGAYGSHYDMMKNFD
                     LIVLAPQVNSYLDDLRKDADKLGIKVIATKGAEYINLTRDPKGAVDFVLKQF"
     gene            complement(15298..15621)
                     /locus_tag="IV334_001444"
     CDS             complement(15298..15621)
                     /locus_tag="IV334_001444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HAP8943518.1"
                     /translation="MDRKELQLLGFEIVAYAGDARSSLLTVLKEVREGNFDNVERLLA
                     DADENLTLAHNSQTKCLAEEASGNEMEVGVIFIHGQDHLMTTILLRELINDFVELYKE
                     KFAKA"
     gene            complement(15644..17050)
                     /gene="lacG"
                     /locus_tag="IV334_001445"
     CDS             complement(15644..17050)
                     /gene="lacG"
                     /locus_tag="IV334_001445"
                     /EC_number="3.2.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-galactosidase"
                     /protein_id="HAP8943519.1"
                     /translation="MNYKTLPEDFIMGGATAAYQAEGSTKEGGKGQVAWDTFLVEKGR
                     YTADPASDFYNQYPVDAELCKKFKIDSIRMSIAWSRIFPKGYGEVNQTGVEYYHKVFA
                     ELNKHGIIPFVTLHHFDTPKELFDNGDFLNRENVEHFVNYAQFCFDEFSEVHYWSTFN
                     EIYPVASNQYLIGSFPPEVQFDIEKTVTCLHNMMYAHAKTVNIFKDGGYKGEIGVVHS
                     LETKYPASDKEEDKHAAKLDDALSIKFLLDATYLGYYSDETMAHINEILAANGQSADI
                     DSNDFREMKKAANRNDFLGINNYGSHWVKEYKGESTLHYNTTGEKGSQVYCIKGIGER
                     IVREDLPKTDWDWIIYPKGLYDLLVRIQDEYPNYNKIYITENGLGYKDNFEDGIILDD
                     DRIDYLRQHFNAISDAIKMGVNVKGYFIWSLMDVFSWVNGYTKRYGLFYVDFDTQKRY
                     PKESAYWYKFVSETKTII"
     gene            17517..18524
                     /locus_tag="IV334_001446"
     CDS             17517..18524
                     /locus_tag="IV334_001446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010780432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAP8943520.1"
                     /translation="MVTIRDIAKKSGYSTATISRLFKGDESLSITSDTKNKIIVTAKS
                     LGYDLTKIKTTLYKIAILFWISEEQLKKDVYYQHVKDDLKNYSKKANLDLIFISKNDG
                     VKSIPDDITGFIAIGQLTKKELYYLNQKGYKGVIRGINPLPEHFDTVEPDFHEMARQA
                     INYFINEGFTKIGFIGGNFFNLETGKEEIDSREYAFRYHLEKSGLLNEKYIFSQGSFT
                     VDEGYQLTLKMKAELNEDMPEACFIPSDTIAAGVLQGCHELGINIPKDMAVISINDDD
                     IAKFVAPPLTTFKIDIEEIAKTCVDLLSDQLVYSRDISKKTLIGAKLTVRKSFIPKEE
                     V"
     gene            complement(18918..>19299)
                     /locus_tag="IV334_001447"
     CDS             complement(18918..>19299)
                     /locus_tag="IV334_001447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="2-dehydro-3-deoxyphosphogluconate aldolase"
                     /protein_id="HAP8943521.1"
                     /translation="NSLRYLEFREGAFDFQTSQQNLLTIPGAMSPTEIFKMFEMGADI
                     VKVFPANCLGPEYFNQVQAPLGSLPLMAVGGVDQTNAQNYLNNGASYVGIGSKFFEKS
                     AVHQLNYERLMELAESFIDSLRVE"
BASE COUNT         5900 a         3995 c         3186 g         6218 t
ORIGIN      
        1 cttttagaaa aagcaaaaaa aaaagaaaaa atcttattct tgataacaag ctacgtgacg
       61 tatatgccta acaaaaatag aacttttgaa tcaatcttaa atgaaggttt aaccacagaa
      121 ccggcatcca ttatttttga tgaagttatc gatgacgttg aagatagtaa gtatcataaa
      181 aatttaaaag aactttttta ttttgcactc aacaatggac caagtttgtt cactggtata
      241 tcagtacctg atagtgataa tccattaatt atggcaaaaa tttacttaac aaaatggtgt
      301 aataaataca tcagggatag gaataaccca gcattaaaaa aacctcttaa aacatttggc
      361 gaaaaagatg ctgctttaac tactagagta gcagcaaatg ctaatattga tgatcaaaaa
      421 gtattgaatg actacttgcg tggacatttt ttatatatga gcgctgagaa catgaacgga
      481 agtattttag aggaatattt agccgaagtg ttagaacctg aaggttggat ttggtgcgcc
      541 ggctctgtat atcgagctgt tgatttctgt tatttaggaa cctcaccaat tcttttacaa
      601 gtgaaaaata aatacaatac agaaagcagt tcgagttcag ctataagagt aggaacaaca
      661 attagaaaat ggaatcgttt aaataagtcg acaaaaattt ctggtttgga tagccctatc
      721 cccaattgga aagcattaat agaaatgact gaagctagta aggaactggc agctaagtta
      781 actgaaaata gttatttagc ttatattaat gaaaaatcca cacgagaatt atggacactc
      841 gatgactaac aatatcctta ttagtatcta tccacgatac atggaaaaaa ttataaacaa
      901 acaaaaaaac tttgaattta gaaatttcta tcttacctca gatccttata atatcttttg
      961 gatttacgaa acaatcccca ccaaagcttt aaagtataaa ttgattgtaa aaaatccaat
     1021 tactaaactt aagaaaaatc aacactactt tttaggagac gatgagtttg aacaattagt
     1081 ttaaaaggga aaatatgctt ctatcattac aagctcgttt ggtgtaagat gggtataagt
     1141 catttatgtt cactctcctt gtatgcttta gcgggtatta caatttgagt gtaacataaa
     1201 tggctttttt atttgtctcg cttaattata caaacggcga attataaata actcataaaa
     1261 aatattttga ctttcaaaat cacttttgct accagaagtt gaggcgaagt gttttaagca
     1321 tagttgtacc ctagtttgac cgttaaaaat aaataacgat cgctagagtg caactatgtt
     1381 tgtttgccct accgtcagcg tcggcttggc ctctcacatc cattaccgtc gggattatct
     1441 ccgttacata cccttacaaa atacgccctg taagcattgc atgaaaccac cgtgcatttg
     1501 caccgtattt ccgccctagc cgttcaaatc ggtcgctacc accattcaga aacacgtgcg
     1561 gtaatggaag gtttggagga agctatccag accactaacg attgtcccta tcgtcgtacg
     1621 tttcactcag atcaaggttg gtcgtatcaa atgaaagctt atagaaacaa attgaaggag
     1681 tacaaaatct ttcaaagcat gtctcggaaa gggaactgct tagataattc acttatggag
     1741 aatttttttg gcttattaaa acaagagatt tttcacggga aagtttataa ctgtttcgag
     1801 gaattaaaat cagccatcga tagctatatc tattactaca ataatgaacg gataaaacag
     1861 aaattaaact ggcaaagtcc cgtccaattt cgtaaaacaa ccgttacggt tgtgtaaaaa
     1921 tatcatttta gagcatgaaa aaaagagtgg atttctccac tccaaaaaag tctaactttt
     1981 gggggtcact acatttttga tcctttttat ttgattaatt atatccagct ggataataat
     2041 tttgggggca acatttctat cttactatta tcttcctaaa gtagtagact ttcttattgg
     2101 gaaaaattac aacaaaaagg agtggcttag ttgacaactc ctttttgttt acaaatcaat
     2161 tggatatcta taaattgtat tttctaacga attttataga catctgattt gtcaaattaa
     2221 gctagacaat atgtctttat ctatgcagct caaaaatact tccaatggtg ttcgataatt
     2281 taatgatttt cttggaatat ggtttctttt cgaggcaacg gattgaataa aggtttcatc
     2341 cgtgtcccta aagtccattg ttttaggtaa gccatcctgc ttgagcccat gcactcggta
     2401 ctgatgagaa aaaattgatg cctgtttctc ctgaggaatg ccaattccct gatcttttac
     2461 ttcaataatc acgctaccct tttggtttga aatggtgaca attaccagtt ttccttcacc
     2521 atatttgatt gcattgtcaa ctaaattcga aaccgcccga taaatccaac gatctcgtcc
     2581 atgaatcagg gttggttgat cttcagtaaa cgcaaacaaa agattcggat aattcatttt
     2641 gtaggcatca caaacttccg cacagatcag tgaaacgtct acgacttccg tgctaagatc
     2701 ctctgagtta ttggataatg tcaaaatatc gtcaatactg tcggtcatcc gatctaaaag
     2761 cattaaattt ccttgatcgg catgttgccg ttctatttcc agattcgtac gcaaaatagc
     2821 gatggcattt ttctgctcgt gggtcagata actatttaat cgtcgataat cctccaaatg
     2881 accatcgaac ttatccttta gcttattaaa agccgcattt aatgcctcgt ccttcacagt
     2941 atttttagga acttgatcaa taaacgttaa atcacgcgta atcgctaaga tttgattact
     3001 gtaaattttg cgtaaaacca accaaagaat aaacgttaat aacatgacgg cagaacaaat
     3061 aaccagcacc atcgctggta aatatttaaa tagttcgtcc tggtaagctt tatctatcat
     3121 cgaaatagtc ataaattgat tgttggaaga tttcgaagct tccatctgcg tctctgcttg
     3181 aaccgtctta ccctcttcca taaaataaac atcttcacct aaggtagatg cgatactgat
     3241 tccctccaaa ttatttctca gaaagaagaa actcactaaa atcacgcctg tcaccaacag
     3301 tgaaaaagcc agcatcgtta acgtcatttt aaaatttaat agcatagctg gtaccccttt
     3361 gctcgaatgg tttttaagac ctctttttca gctgcttcag ccagtttctt ttttagacga
     3421 gccagatgca ctcgcagaac cgacgaaaaa ggatcaaaat cttcatcgta gacatgttca
     3481 gaaatttcct cagacgaaac aaccgctgga tgacgctgag cgatgcacag taagatatca
     3541 aattccttgg gtttcaggac cacctctgtc ttttgatagc tgacccttcg acctaatgga
     3601 tcaatcttca aagcaccaat tgaaatctgc ggcagcgcac gtccatgata acgacgaata
     3661 accgcgcgta ttcgtgcttt taattctagt aactcaaagg gtttgactaa ataatcatct
     3721 gctccaaaat ccaagccttt tgctcgttct tcaatctcat cacgtgcagt aataatgata
     3781 actggacttt cgacttcttc agcacggaga ctttgtaaaa cagataatcc atctttttta
     3841 ggcagattta aatctagtag tatcccatcg tatgaattga tgaaggcctt ctcttctcct
     3901 gactcgccat cataagctac atctacagaa atattctcct gctctaggcc cttttttacg
     3961 gataaaacca gctcacgatt gtcctcaacc accagtacac gcatgtatat tcactcctca
     4021 tacggattta gtaattacac ttatgctttt tagcatagta cacgctcttc ttcaaagtcc
     4081 acgttctttt tgtgaagatt tgaagaaatt catacattct gttaaaaaac acataattaa
     4141 gaatgatttt actgtttgaa aatacctaga atagaaaaag actggaacct ttcctccagt
     4201 ctcactactt tctatttgct tggttttctt gtatatactt ttcaaagttc gttggtttat
     4261 gaccaataag cctcgttaga atactgaaaa ttgccatcaa gtcattaagt accacatgta
     4321 agaaaatctc tctaaagaaa aaccccttcg ttttaactca cttactattt tagaattcgt
     4381 tcccctaata atgcagcatg attttacgct ttatgtataa aaaaagactg caagcaattt
     4441 tgcttcacag tctgatttct tatgatagtg agcttgttaa cacttccacc ggtgtagcag
     4501 tttctttcaa tacggcattt aattcttcaa tatttttacg cccttgcgta ttcatccact
     4561 ccatcgctgc ttcttttcca tcaacgatat agctttctac accatctgcc caagtcgcac
     4621 ggccgcaaag tacaccattg aaggttgagc cggcattttt cgcgaagcgc agtgtttctt
     4681 ggaacaactc tgcttttaca ccggcgctta ggaaaataaa tggcaggtta gtggcttcag
     4741 cctgttcctt gaagaagtta gccgcttgtg cctttgtgta cacgacttcg tctgtcccat
     4801 agccttccac ataattcata ttgacgggta cttccacctt taacacatct acgccataac
     4861 gtggatcaga aaattccttc atcatatcgt tgactttatg aggtttctta atggcgtatt
     4921 ctttcgaagt cgcatcagag atgttcgcgt cataagaaac aagctccaag aagaagggta
     4981 agttttcagc ctgacattct gagccgattc gttcaataaa ggcatgtttt tgattattga
     5041 tttcttctgc ttcatcgaca tcatagtaaa gtaggaactt acaagcatcg gcgcctgctt
     5101 cttttaaacg tttaactgac cagatcgata ataaatctgg taaacggccg ggagttgacg
     5161 catcgtaacc agttttttca taagctaaca gcaagccggc attttttgct ctaacgtcag
     5221 ctgctggcaa gccatattcg ggatctaata aaatcgatga ggcatatttc gttaattgtg
     5281 aagaaacaat ttctttaaat tcaacgattt ctgattcttc tgcttcgctg cccttgaatt
     5341 ttgtgatcat cttcttcaaa gccccgcgtt gatcaattgc taaggcccca atcactccat
     5401 cttcattgca tagttgcttg atatattgac gtttttcttc gttcatttcc gtatcctcct
     5461 acactttctc cactaagatt tgatcatata gtttttgata attggctaat tggacatagc
     5521 cagtcgtcgc ttcctgagca ttcagcattc ctaaaacatt accctgtttt aacacggctt
     5581 catccgaaag accttgttga atcgcagcgg tcaatccggc aacggtcgca tcaccagagc
     5641 caactggatt gacgacatca atctttggaa ttgtcacgca ataaaaagtc tcgtgatgtt
     5701 tggcaaaggc tccgtcgccc cccattgaaa ccactatcca ttcaatttca tcaaacatcg
     5761 aatctgacaa ggcggctttt aaatctgata attctgttga gatttctcgt cctagcagac
     5821 cagccagctc ttcggtattg ggttttatta gcttaggctt tacactgcct cgcaacactt
     5881 cccttaatgc tgtacctgaa caatctaaaa cgacaggctt tggctgacaa acttgcagca
     5941 tttgtcgata aaaatcagca ggcaatcctt ctggcaagct tcctgaaaaa gaaatgattt
     6001 cggctttaac agctaatttt tcgaaatgtt gcaagaacgc ccttgcttct tgttcagtaa
     6061 taacaggtcc gttttctagg atttccgtct gttttccgtc atgtaaaaca gcgatgcaat
     6121 tacgtgtctc tccgctgata ggtaaaaagt catgagcaat ctcattttga gtcaggtcct
     6181 ttttaatctc ttcacctaaa aaaccgccga ttaaaccact agcaatcacc tccgtatcta
     6241 attgcttcaa gatacgtgtc acatttaggc ccttaccgcc agctgttttg cgaacatttc
     6301 ttactcgatt gactgtgtcg agcttgaagg tttctaatgg ataagaaata tcaatcgatg
     6361 gattcatcgt tattgttaaa atcatttgtc cacccgctta atcgtggtat tcgccacgat
     6421 cccatttctc gataaattca tcgaagaaat gcggatcaga ctgcttgtca ctggcttttt
     6481 cgacgtgatc gattttggcg atcaattttt tgttttcttc agtttcttga taatcggcat
     6541 tgatgaaggc ctctaatatg tcacagatca aaaattctcc cgtaatccga gcgccaaagc
     6601 cgatcacatt ggcatttagt tgttccttcg catagatagc agtcgtcata tcgcgaacca
     6661 gcgcactgcg gataccaggt actttattga cagcgttgtt aatcccaaca cctgttccac
     6721 acaaacaaac tcctagatct gcttctccgc tagtcacggc ctctccgact ttttttccgt
     6781 agatcggata atgggttctg gtaaagtcat aggtccccat atccaacact tcatgtcctt
     6841 ttgattttaa gaaatctgat actgccattt ttgtatctgt gacgatgtgg tcacagccaa
     6901 ttgcgatttt catttcattc atccttccta attacgccat tttatttaac atgtcgacac
     6961 gtacttgatg acgaccacca tcataatttg cttgtagaaa ttccttaaca atattcaacg
     7021 ccaactcatc gccaacaatt tttgatccaa gtgtgatcat ccgagaatta ttatgtttac
     7081 gggtcatata cgcactgcgc tcatccgaaa cttctgctgc taccattcct ttaacctttg
     7141 ttgccgtgat aaagctgcca gcgccgtagc catcaaccgc gattcctaaa ctaccttcgt
     7201 tttcattcag atcatttgcg atcgccaaag ttgattccac aaaatcttct gaagccgttt
     7261 cactcttatc tactactgta ataccttctt tgatcaattt tttcttgatt aattccttta
     7321 attccatgcc gtcactgtct gaaccaatta ctacacgcat ttttgtttcc tcctattttt
     7381 tactgaatga ttttagtgaa ttgctgataa taactcttca gctcggctga aagagtagaa
     7441 tccgtaaata atttgtcgat cttgcgcaaa tcatagaaat tataaaaatc gctatggtcg
     7501 aatttgtttg catctgaaac caaacatttt tctgccgcat tatctaaaac aattttttgc
     7561 aaggtacctt cttcaatact gaaattcgaa acctccgaac ccttcaatcc attcactccg
     7621 acaaaagcct tcttaatacc gattttttga acagcttcat tggcaattga accgacaaag
     7681 gctccagtct tttcccgata tagcccaccg atcaaataca gttcaaatct ttttatttca
     7741 cttaatagat tgaaaaccgg cagactattg gtaacgattc ggagagatga cggcttgatg
     7801 tatcgtgtca ttaattcgat cgttgtaccc gatccaagaa aaatcgtgtc gtcttcttga
     7861 ttgatatgag ctgccgcatt tttagcgatg tattccttct cttttgtatg cagcttcttt
     7921 ttttcagtat gagaaagctc atttgcttct atagaagtcg acatttccag ataatcgatt
     7981 ttttgtgcac cgccatgaac gcgtaaaagc tttttcttct gcgcgagttc ctctagatct
     8041 cggcggatcg tcatatctga aacattcagt acttcctgaa taaatttgac tgttatcgtc
     8101 ccatagcgat cacacatcgc taagatcgaa cgctgtcgtt ctgttttcag catattctgt
     8161 acctccacga ccttataata aaacatttac gaacattttt caacacatat tgttcgttta
     8221 ttttcgttta tgcgttgaca atgattttgt aagcggttat actcaacttg taaacatata
     8281 aataaacata aacgaactaa taaaggagct gttttgtgtg aatgaattct ttttcaaaga
     8341 cactgtctat gtttctgaca agcagtcgca ggaagaagtg ttcgaggaag tctatcaaga
     8401 tctgttaaag aaagaattgg taaccgaaga ttttttggag aaccttttag aacgggagcg
     8461 taattatccg acgggattag atctctcacc tgtctctaaa atattgccag atatcgctat
     8521 ccctcatacc gaaagtgagt ttgtgcgaac cacccgtatc attccaatca aagtaaaaaa
     8581 tgaaatccct ttctacaaca tgatctcgcc aagcgatcag atttccgctt catttctttt
     8641 catgatcttg aacgaaaatg gtgaagcaca ggctggcatg ttggctaata tcatggattt
     8701 cattaattct gttgacgaag aaaaactgtt ttcgtttttc aaattagagg acacagaagc
     8761 gatttatcat tttttagaag aacattttta tatatagaag tctctgccta accgcaggat
     8821 aaaaaataaa cttattttaa ggggagaatt attatgatca aaattttagc agcatgtgga
     8881 gcaggcgtta attcaagcca tcaaatcaaa agcgctttgg aaactgaatt gaaaaatcgt
     8941 ggctatcaag tcacctgtga cgccgttatg atcaaagata tcaatgaaga aatgctgtct
     9001 cattatgaca ttttcgcaca aattgccaac accgacttgg gttttgacat taacatccct
     9061 gtcgttgatg caggtgctat tctttatcgt attcctgcta tggctgagcc tgtttaccaa
     9121 agtatggaaa gcgcaatcca atcattaaac aaataggact aaagaaagga aaagcatatg
     9181 aatacaatta ttgatattgc caataaagta tttacccccc taattaattt aggtgcggca
     9241 ccgttaatga ccatcgtatt aactgttatt gctttatttt ttaaagtaaa accgtcacgc
     9301 gctcttgaag gcgggctcaa attagggatc gccctgactg ggatcggagc tattattgga
     9361 attttaacta ctgctttttc agacgctatg acagcttttg ttgaacgcac aggtatttca
     9421 ttaaacatta cagatgtcgg ttgggcccca ctagcaacca tcacatgggg gtcgccatac
     9481 acgctttatt ttttactaat tatggtgatc gtcaatattg tcatgttagc aatgaaaaaa
     9541 accaacacat tagatgtgga tattttcgat atttggcatt tagcaattgt tggtttgttc
     9601 gcgatcttct gcggtgctaa tctgtttatt gcttctgcat tagttatttt cattggtgta
     9661 ttaaaaattt ggaactccga cttaatgaaa ccaaccttca atgacttgtt aaatgcgcca
     9721 gacagcaacc cgatgaccac gactcatatg aactacatga tgaatccgat tattatggtt
     9781 ttcgataaaa ttttcgacaa aatcttccct tggttggata aatatgattt cgatgctgcc
     9841 aaattgaata gtaaaattgg cttctgggga tcaaaattcg cgattggaat ttatttgggg
     9901 atttttgttg gattactagc aggacaaaca ccaacgcaaa ttttctcgct agcctttacc
     9961 gctgccgtct gtttggaatt gttctctttg ataggcgctt ggttcatcgc agccgtagaa
    10021 ccgttatcac aagggattac cgattttgcc aataaacgac taaaaggacg taccatcaac
    10081 ataggtttag actggccttt catagcaggc cgcgctgaaa tctgggctgc cgctaacgta
    10141 ttagcaccaa tcatgttgct agaagccatc atcttaccag gaaataaatt attgccacta
    10201 ggcggaatca tcgcaatggg tgttacccca gcactattgg tcgtaactcg tggaaaatta
    10261 atccgtatga tcgtaatcgg cgcgatcgaa ttaccattat tcttatggtc cggcaccttg
    10321 atcgccccgt tcgttactca aacagctaaa gcagtcggag cctttccagc cggcttgaac
    10381 gggtccgcca tgatctctca tacaacgatg gaaggcccga ttgaaaaatt cctaggttat
    10441 ttagtcggta atgcttctca aggacaaatc gaatttgtga tatacgctgc cttagcatta
    10501 ggtgcatacc ttttaatctt catttggtac gctcgtcaaa tgaaaaaacg caacgctgca
    10561 tacgcagctg aaaaagaaga gaatgcatct ccggcaacgg cgcataatat gacttatgca
    10621 gccgaagcta aataagctaa aaacaccccg aatgtcttta gacttcgggg tattatttta
    10681 cctataaaaa ataaatagat gtaaaatcca tttcaaaaag catagcccat ctcttgccac
    10741 acccaataaa gaaattatca acgtttatgc tttttttaac tcctttaagc ttgcctttgt
    10801 tggtattcct caacgattct ttttgtgttc ttgtttgttt tgtgcttttt gactttttgt
    10861 tgtttaattg cgaattcctt gatttctttt ttcctgaatc gcttcgcatc ccgcggttat
    10921 gcgtgcgatt tcatcattgc tttttctgat ctaactcgtt tcatgtcaaa gagcttcagc
    10981 tgacttagtg tccatggttg ttttgatatc ccttttcctc cttaataaag cggtgattga
    11041 tactgctatt ttttccttca cataaacata acatcccaaa aaaatcgact atcttttttc
    11101 aaatggtgta ttcaagtatt cggaggaatt agtaatgatg caccctaaaa ataacataat
    11161 tcgcttaaat tcaaggctac caaaaaacta ccaagttcta attatctaga gtttggcagt
    11221 ttctcattta cacaacatat ttttcttctt tttcgatttt ttcatagtgc ttttgttcat
    11281 catcatccgt tataactact gttacatcat tcagattata atatactgaa aaatctcttt
    11341 tcccaatttt cgaatgatca atcaataaaa agcgttcaac agaattattc agagcgatct
    11401 gttgagtttg gccctcttca aaagtcgaag tcataatttt ctgttcattt aaagcattac
    11461 agctgaaaaa tgctttatga aaattcatat cctgtagcga tttattcgtg atttcaccaa
    11521 caaagcattg agtcgtttcc cgcatctcgc cacctaatag atagattttt cgattccctt
    11581 ttcgcttatt taattcttca aaaacaggca aacagtttgt aacaatccgc agtgaatccc
    11641 cttgaatgat ttttgacatg agctcgattg tagttcctgg tccaagaaat atcgtatcac
    11701 catcattgat catttcattc gccttttcca caatccgctg cttttcttct tgcagcataa
    11761 tctgtttttc tctatgagaa agctccacat gccgataaaa atccttcaat ttcgctccgc
    11821 catggacgcg ttctaataat ccttgtttct ccatttcgtc caaatcacgt cggactgtca
    11881 tatccgaaac cgaaaacttc tccataatat cgctgactcg tactgtaccg ttaacctcta
    11941 ataacgacat gatcgcttgg aatcgatccg tttttaacat ttcatcgcct cttttctttt
    12001 taagactatc aaattcttaa gaaaagtgct agaacaagtt ttcttaagaa gccttctgca
    12061 ggaaggaaag caaaattttt cagaatttgc aagctgctat tctaaaaaat aaaaccgaac
    12121 gagcatgact gtcatagtga cagccttgtt cattcgactt ttatctttaa taaatcattt
    12181 tatttaatct gaagttattg tgcaagttca aaaacaaagt agttaatgtc tcctcgatat
    12241 ttcgaaatcg aatactcaac gggcagacgt tctagcgaaa aaccctgtgt atggaaatag
    12301 aacattggat ctccttcttc cacatctaac aaatcgctga tcgtatcatc cgctttgatt
    12361 acttctagtt ttctggtgac cgttttaatt ggataaccca tttcttcaaa aacttgatac
    12421 attttttctt ttctgatttg tcaaattaag ctagacaata tgtctttatc tatgcagctc
    12481 aaaaatactt ccaatggtgt tcgataattt aatgattttc ttggaatatg gtttcttttc
    12541 gaggcaacgg attgaataaa ggtttcatcc atgtccctaa agtccattgt tttaggtaag
    12601 ccatctcgtc taagcaagcc gtttgagtgc tcatttagcc ctctttgcga aggtgttcct
    12661 ggatctgcga aaaagatggc aatatcgttc gagttactta tagatcgcca gttggaaaat
    12721 tcttttccac aatcaaatgt catggattta aacaagtgtt taggcatgga tttgaaccaa
    12781 tcatttaacc tgttttcgat atcttcggct cgtctgcctg tcggtttcaa agtaataata
    12841 accttggata aacactcaac caatgtaata acagcgctct tgtgtttaac tcccacaatg
    12901 gtgtcgcctt cgagatgacc aaattcttta gtcaggtgtg gatagagact gttccgttca
    12961 tgaatcgttc tgcgaaaggc ctgttttcca cgcttttctt gatgattatt cggtttacgt
    13021 ttccccttca taggtaacga ctgtgtgtca aaaaggcctt ttttaaacat ccgatagagt
    13081 gtacgaacag aacaatcaat tggaaactcg ccacggccga taatcacatc gggtgaccac
    13141 ccttgagcta ccttttcttg aacatatgtt gattgttcat cagataaaac ggttgaacgt
    13201 cgtccacaac gagatttatt ttgtttgtat tgacggtaat aatctagcac ttttttcctt
    13261 gtttgagaaa cgaataaaca ttataaatgg tttgtctaga gcgatttaaa ctttgtgcga
    13321 caagagcgac aggtttctcg atattgaaat aagactctat gattactagt tcgtctgttg
    13381 taagatgggt gtaggtcatt tgtactcact ccttattaat tctttggtcg gaactatttt
    13441 gagtgtagca caaatgatct ttttagttgt ctagcttaat tttacaatcg acgcctcaaa
    13501 aagaataccc gaaatttaaa cagcaaaaaa agatagcgct taccattgtg tttaagataa
    13561 aacgctagct aagttggcta gcgctttatt aatctaagca agctctaaaa ttgcttcaat
    13621 acaaaatcaa ctgctccttt aggatcacgt gttaaattga tatattctgc acctttagtt
    13681 gcaattactt ttatccctaa tttgtctgca tctttacgga gatcatccaa ataggaattc
    13741 acttgaggtg ctagaacgat taaatcaaaa tttttcatca tatcataatg agaaccataa
    13801 gctcctgctt tagcagaaag attaacacct gtctcttttg cgccctcttc taatgcatta
    13861 gccaacattg cactcgttcc ggcaccagca caaagtacta aaacgcgctt tccttcacca
    13921 accgatgctt ttgcttcatc aactataact gccgcagttg caccaacttc ttggatttct
    13981 tcgaccaatt cttcttttac ttcttctgca gcaatgtttt ttaactcttg ttctactaat
    14041 tcagaatcat aggccttaac aaaaggtaaa taaataatcg catcgacaac aatcaatact
    14101 accgctaata caatcgccaa cacactaaac ccggttccta aaaccaagcc aatcggtgcg
    14161 ggcgtcgccc aaggcaatac ggatataaag ctattcatcc ccaagacatc gacaaaaagt
    14221 ttgaatatcc atacgttagc gattggtgct aataaaaatg gaattagaaa gtacggattc
    14281 aaaataatag gagtagcaaa tagtaacggt tcatttaccg caaacgaaac tggaataaat
    14341 gaagctttac caactgcttt caattgtttt gatttagcga acaacatgaa cagaaaagga
    14401 acaactagtg tcgctccagt accacccatc gttccaacaa agtttcctag accaactgtt
    14461 aatacttttg aggcttgtgc accgttttta aataattcaa agttagtttc tacgttcgca
    14521 taaataattg cagcaatcgc tggttcaaca atcgatggtc catgaacacc aacaaaccag
    14581 aacattgcca tggctcccca aatgattgca ataccgacat atccatcagc agcactaaat
    14641 aatggctgca ataattgaat tacacctgct gcaaatgatg ttccaaaaaa ttgacgaact
    14701 cccgtatcaa tcaaaacggc aataaatact gcaattgaaa aggggataat atctctaaac
    14761 atctgtgtga ttgttccggg aacttctttt ggcatacgga tcgtcaaatc tcttttaaca
    14821 cagaatttat acacatttac agtaacaaac gcagaaacga aagctgctaa taatcccttt
    14881 gtccctaaat aatcagtagc tatcccacca tcaatttgat cagctgccat cattacaaaa
    14941 ccacaaattg acgctaacat aacagaaact tcattaatct gcttacctga atcagataat
    15001 tgacggttca ttgaattagc taaacatcga gctgttgtag caacaaccaa aagaccaact
    15061 aatcccattg aataaccata aactttccaa agccacgtcg agaatgcctc tggtaacgtc
    15121 acgttaaata cttctggaac agccgcaatc ataataaaaa tacttgaaaa tagaatagct
    15181 ggcatagcag ttaaaaagcc atcgcgaatt gcccctaaat aaatattacg tgagattttt
    15241 tcaaaaaacg gttttccctt ctcaatttgc ttaataatcg tattcatttt tcttctccta
    15301 tgctttagca aatttttctt tgtacaattc aacaaaatca ttgataagtt ctcttaatag
    15361 aatcgttgtc attaaatggt cttgcccatg aataaaaatt acgcctactt ccatttcgtt
    15421 ccctgacgct tcttcagcta aacatttcgt ttgtgagtta tgcgctaagg ttaaattttc
    15481 atcagcatcc gctaataatc gttcaacatt atcaaaatta ccttctctca cttccttcaa
    15541 cactgttaat aatgaacttc tggcatctcc tgcataagca acaatttcaa atcctaataa
    15601 ttgcaattct ttcctatcca tttccttgcc ctcctaacta tttttatatt atcgtttttg
    15661 tttcgctaac aaatttatac caatatgcag actctttcgg atatcttttt tgtgtatcga
    15721 aatctacata gaatagtcca taacgcttag tgtatccatt aacccaagag aaaacatcca
    15781 ttaatgacca aatgaaatac ccctttacat taactcccat tttgatagca tcggaaatcg
    15841 cattaaaatg ctggcgtaaa taatcgattc gatcatcatc tagaataatc ccatcttcaa
    15901 aattatcctt ataccctagc ccgttttcag taatgtatat tttattataa tttggatatt
    15961 cgtcttggat tcgtacaagt aaatcatata acccttttgg ataaatgatc caatcccaat
    16021 cggttttcgg aagatcctca cgcacgattc tctcgccaat ccctttaatg caatagactt
    16081 ggctaccttt ttctccggta gtgttgtagt gaagtgtgct ttctcctttg tactctttaa
    16141 cccaatggct accatagtta ttgataccaa gaaaatcatt tcgatttgct gcttttttca
    16201 tctctctaaa atcattagaa tcaatatctg cagattgccc atttgctgct aaaatctcat
    16261 taatatgtgc cattgtttca tctgagtaat accctaaata agtagcatct aataagaact
    16321 taattgataa cgcatcatct aatttcgcag catgcttatc ttcttctttg tcacttgctg
    16381 gatattttgt ctctagcgag tgaacaacac caatttcccc tttatatcca ccatctttaa
    16441 aaatgtttac tgtctttgcg tgcgcataca tcatgttgtg taaacaagtt acagtcttct
    16501 ctatatcaaa ttgaacttct ggaggaaacg atccgatcaa atattgattt gaagcaactg
    16561 gataaatctc attaaatgta ctccaataat gaacctcaga aaattcatcg aaacagaact
    16621 gtgcatagtt tacgaaatgt tcaacatttt ctcgatttaa aaaatctcca ttatcaaata
    16681 gttctttagg ggtatcaaaa tgatgaagag ttacaaaagg aataatacca tgcttattca
    16741 attcagcaaa taccttatga taatattcca ctcctgtttg gttcacttcc ccatagcctt
    16801 ttggaaaaat acggctccat gcaatagaca tcctaatact atcaatttta aattttttgc
    16861 ataattcagc atcaactgga tattgattat aaaaatcact agctggatca gcagtatagc
    16921 gtcccttttc tacaaggaaa gtatcccacg ctacttgccc ttttccacct tcttttgtag
    16981 agccttctgc ttgataagct gccgtagctc ctcccataat aaagtcttct ggtaaagttt
    17041 tataattcat tttaatctct ccttgaaaat tagtttgatc aatatgccaa taatccataa
    17101 atcacctcag cataatttta acatgtataa gtaaattttt ttacacaacg aaaaaccgac
    17161 ctcgaatatc tattttattg cgatatacaa gcacttatat cccttgatgt aaaataaata
    17221 taccattcaa aaaccgtttt gtaaacgttt ttttgtaaaa taaatttatc tttttactaa
    17281 tttaatttac acgaaacaag aaaacggact gattcccact aaaatcaatc ccacctacta
    17341 atcattctcc aagtggctat ccattatcga ttcaatcttc tctgttaatg gattaggctc
    17401 tctgctgata tcagagtttc gtttcctgct agaaaaagta atttaagtta ctaaaagagc
    17461 agtttcgatt tactttaaca aagaaaagga ttattatatc cctaggagga tgaacaatgg
    17521 ttactattag agatattgca aaaaaatctg gttactcaac ggctactata tcaagacttt
    17581 ttaaaggtga tgagtcactc tctattacat ctgatactaa aaacaaaata atcgttacag
    17641 ccaaatcttt aggatatgat ctgacaaaaa ttaagactac tctgtacaag attgccattc
    17701 ttttttggat ctcagaggaa cagttaaaaa aggatgtcta ctatcaacat gtcaaagatg
    17761 atttgaaaaa ttatagtaaa aaagctaatc tagatttgat tttcatctct aaaaacgatg
    17821 gtgtaaaaag cattcctgac gatattactg gatttatcgc aataggacaa ttgacaaaaa
    17881 aggaattgta ctacttaaat caaaaagggt ataagggggt aatccgagga ataaacccat
    17941 tacctgaaca cttcgatact gtagaacctg atttccacga aatggccaga caagcaatta
    18001 actactttat taatgaaggt ttcacaaaaa ttggatttat tggaggaaat ttctttaacc
    18061 tagaaacggg taaagaagag attgatagcc gtgaatatgc cttccgatat catttagaaa
    18121 agagcggact actcaatgaa aagtatattt tctcccaagg atcatttact gttgacgaag
    18181 ggtatcaatt aactcttaaa atgaaagctg agttaaatga agatatgccc gaggcatgtt
    18241 tcataccatc tgacactata gctgccggcg ttcttcaggg ctgccatgaa ttgggtatta
    18301 acatccctaa agatatggca gtaataagta ttaatgatga cgacatcgca aaattcgttg
    18361 caccaccact tacaactttt aagatagata tcgaagagat agctaaaacc tgtgtagatt
    18421 tattaagtga tcaactggta tattcgcggg acatatcaaa gaaaacatta atcggcgcaa
    18481 aattgaccgt taggaaaagt tttatcccta aagaagaggt gtaaaataag taattgaaac
    18541 ttatctttaa aactataatt gtgtccacat agtagccgag caatcgggtt tatgcctatt
    18601 atgttttcca taaataattt gatggtgata ttttgacgta aatacaatat gattttttca
    18661 tcgccaaatt gtgcgcgcta atttttgttt atcattcaga acatttcctc ctttttatct
    18721 aagtatgagg ttggataaac gcttcatctt agtatcttgg gggatttttt atctcgttgg
    18781 gaatatatca agcatcagag cgcgcaaaaa gaactggtcg aaaaatcaat ctaccagttc
    18841 aatctcattc actgtgatac tatgttttat acagataacg ccatattata aaacaagttg
    18901 tattccgcaa atgggttcta ttcaactctt aaactatcta taaaagattc agccaactcc
    18961 attagcctct cgtaatttag ttggtgaaca gcactttttt caaaaaattt tgaaccaatt
    19021 cctacataag aggctccatt gttcaaatag ttttgtgcat tagtctgatc tacccctcct
    19081 acagccatca aaggtaaact tcctaaaggg gcttgcactt gattgaaata ttccggtcca
    19141 agacagttgg cggggaacac cttaacaata tcagcaccca tctcaaacat tttgaatatc
    19201 tctgtgggcg acatggcccc aggaattgtt agaaggttct gttgcgaagt ttgaaaatca
    19261 aacgcgccct ctctgaactc caaatatctt aggctgtta
//
LOCUS       DACDKM010000043        19276 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20834_2016
            SAMN12640370-rid9891663.denovo.043, whole genome shotgun sequence.
ACCESSION   DACDKM010000043 DACDKM010000000
VERSION     DACDKM010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640370
            Sequence Read Archive: SRR10040891
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19276)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19276)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742835.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742835.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:04:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,012
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 29 of 75
            Pseudo Genes (incomplete)         :: 33 of 75
            Pseudo Genes (internal stop)      :: 19 of 75
            Pseudo Genes (multiple problems)  :: 5 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19276
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640370-rid9891663.denovo.043"
                     /isolate="E20834_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            147..875
                     /locus_tag="IV334_001448"
     CDS             147..875
                     /locus_tag="IV334_001448"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HAP8943522.1"
                     /translation="MEKKELLYEGKAKKIYLTSDENLIWIEYLDQATALNGARKDQIS
                     GKGELNNQITSAIFRYLTQRGIKNHFIEQLSKTEQLAKKVQIIPLEVVIRNIAAGSFS
                     KRLGVPEGKEFIKPIIEFYYKDDQLDDPFINEEHIYFLQLAKPEEISELKAQALAINE
                     ILTELFAKVGLVLVDFKLEFGRSNGQILLADEVTPDTCRLWDERTNEHMDKDVYRRDL
                     GDLIPVYQEVYNRLSELTEEKERV"
     gene            875..1129
                     /gene="purS"
                     /locus_tag="IV334_001449"
     CDS             875..1129
                     /gene="purS"
                     /locus_tag="IV334_001449"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010758866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurS"
                     /protein_id="HAP8943523.1"
                     /translation="MYFVKVYVTYKDSVLDPQGEAVKKAVHRMGYETIEDIRIGKYFE
                     IQLADAEEPIDTVIETICDKLLANVNMETYRYEIKKMEEV"
     gene            1131..1802
                     /gene="purQ"
                     /locus_tag="IV334_001450"
     CDS             1131..1802
                     /gene="purQ"
                     /locus_tag="IV334_001450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurQ"
                     /protein_id="HAP8943524.1"
                     /translation="MKFAVIVFPGSNCDADMLWAVREIMGADAEFVRHDESSLEGFDG
                     VLLPGGFSYGDYLRCGAIARFSTIMEEVIRFAEEGKPVFGTCNGFQILTEAGLLPGVL
                     RRNESLHFICKTVPLKVNPKTAFTSEYEADEIIYLPVAHGEGNYFCDEETLQELKKNN
                     QIAFTYNDNINGSVENIAGITNKAGNVLGMMPHPERAVEDLLGSEDGKRFFASILKNF
                     GKVTV"
     gene            1803..4025
                     /gene="purL"
                     /locus_tag="IV334_001451"
     CDS             1803..4025
                     /gene="purL"
                     /locus_tag="IV334_001451"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurL"
                     /protein_id="HAP8943525.1"
                     /translation="MKTLKEPTAEEIKKKRIYAEWGLTDEEYRMIEEDILGRMPNYTE
                     TGLFSVMWSEHCSYKNSKPVLRKFPTSGPHVLQGPGEGAGIVDIGDGLAVVFKAESHN
                     HPSAVEPYEGAATGVGGIIRDIFSMGARPIALLDSLRFGELDNPRTKYLLEEVVAGIG
                     GYGNCIGIPTVGGEIAFDPCYEGNPLVNAMCVGLIEHKHIQKGQAAGVGNSIMYVGAK
                     TGRDGIHGATFASEEFNQEEEQQRSAVQVGDPFMEKLLLEACLELILHHSDSLIGIQD
                     MGAAGLVSSSAEMASKAGSGLILTLDEVPQRETGMTPYEMMLSESQERMLICVKSGEE
                     EKIQELFQKYDLDAVTIGKVTDDGQYRLYHQGEEVANLPVDALAEDAPVYHKEMKEPA
                     RIVEFQQLAPYQPVIEEPGQVLLDLLQQPTIASKRSIYETYDSQVQTNTVVRPGSDAA
                     VMRVRGTNKALAMTTDCNARYLYLDPKIGGQIAVAEAARNIIASGGKPLAITDCLNYG
                     SPDKPEVFWELSTSADGIAAACETLGTPVISGNVSLYNETDGQAIYPTPMIGMVGLIE
                     DHKHITTQEFKKSGDLIYILGKTFADFDGSELQKMQLGRIEGVIRNFDLSIEKRNQEL
                     VLTAIQNGLIESAHDCSEGGLAIALAESAFKHQLGISVQFELSSAQLFAETQSRFVLT
                     VAPENKTRFEEMMGDAAVLAGKVTDEAIIEISATDGQIKIETAVARKCWEDAIVCLMK
                     "
     gene            4010..5449
                     /locus_tag="IV334_001452"
     CDS             4010..5449
                     /locus_tag="IV334_001452"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010768528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HAP8943526.1"
                     /translation="MSYEVKSLNEECGIFGVWGHPDAASVAYFGLHSLQHRGQEGAGI
                     VSNDRGKLKGHRDLGLLSEVFKDQRKLQQLTGEAAIGHVRYATAGNGSVDNIQPFLFK
                     FHDQSIGLAHNGNLINARSLRKELEENGAIFHSNSDTEILMHLIRRSEETDFLNKLKS
                     ALNEVKGGFAYLLMTETAMIAALDPNGFRPLAIGQMKNGAYVIASETCALEVIGARFI
                     RDVQPGEIVIINDEGIQIDCFTKDTQLSICSMEYIYFARPDSNIAGINVHTARKNMGR
                     NLAKESPVKADMVVGVPNSSLSAASGYAEASGIPYEIGLVKNQYIARTFIQPTPELRE
                     QGVRMKLSAVRGVVEGKKVIMVDDSIVRGTTSRRIVHLLKEAGAKEVHVRIASPPLKY
                     PCFYGIDIQTRKELIAANHTIDEIKECIGADSLGFLSEEGLIESIGLEKDAPYSGLCM
                     AYFNGDYPTPLYDYEEKYQESLKEKVSFF"
     gene            5481..6524
                     /locus_tag="IV334_001453"
     CDS             5481..6524
                     /locus_tag="IV334_001453"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HAP8943527.1"
                     /translation="MANAYAKAGVDVEAGYEVVERIKKHVKKTERLGMMGAIGGFGGC
                     FDLSVFQLKEPVLVSGTDGVGTKLAVAIEADKHDTIGIDCVAMCVNDILAQGAEPLYF
                     LDYLATGKNDPLRLENVVKGVADGCVQGNMALIGGETAEMPGMYSGNDYDLAGFAVGI
                     AEKSQLLTGETINPGDVLLGIASSGIHSNGYSLVRKIFFEQQQFSLSSKLPELKKTLG
                     EELLEPTKIYVSSLLPLIKEKKIKGAAHITGGGFVENIPRMLPESMAAQIKLGSWPIL
                     PIFGCLQKYGEIPEMEMYEIFNMGIGMVLAVEESKVKEIVHEIEQAGEKVYEIGKVIA
                     KEKHRVVFIGEER"
     gene            6521..7099
                     /gene="purN"
                     /locus_tag="IV334_001454"
     CDS             6521..7099
                     /gene="purN"
                     /locus_tag="IV334_001454"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005873397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HAP8943528.1"
                     /translation="MRIAVFASGNGSNFQALADYLSKKGLESSIDWLFCDQPEAYVLK
                     RATALSVPADCFSPKEFDSKKEYEEAILHKLKEKKIDLIVLAGYMRIIGPVLLKNYDK
                     RIINIHPSLLPAFPGLHGIRDAFEAGVKETGVTIHYIDQGVDTGPIIRQEKVRIEQED
                     TFDSLEEKIHRVEHRIYPEVISEIIENGGYLK"
     gene            7096..8631
                     /gene="purH"
                     /locus_tag="IV334_001455"
     CDS             7096..8631
                     /gene="purH"
                     /locus_tag="IV334_001455"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HAP8943529.1"
                     /translation="MTRALISVSDKTGIVAFAQGLIEQGVEIISTGGTKHALDKAGIK
                     TLSIEEVTGFPEMMDGRVKTLHPKIHGGLLGRRDLPSHMEAMETENIQPIDFVCVNLY
                     PFKETIMKPDVEIAEAIENIDIGGPSMLRSAAKNHEFVTSVVDPSDYEEVLSELRQYG
                     HTSLSFRQKLAAKVFRHTAAYDALIAQYLTDWVEEKEPEKLTLTYERKQTLRYGENSH
                     QAAAFYQSALLEKYSIAAAKQLHGKELSYNNIRDADAALRIMKEYDEPTVVALKHMNP
                     CGIGTASTILEAWEAAYEADPVSIFGGIIVLNREVDLPTAEQMHKLFLEIIIAPSYTQ
                     EALELLQKKKNIRLLTVDFAQEENQNREETVSVLGGLLVQDQDLAIENEEEWKVVTKR
                     KPTPAEAKALAFAWRAVKHVKSNAIVLANERQTVGIGAGQMNRVGSVKIAIEQAQEKM
                     EGAVLASDAYFPMDDSVEYAAKHGIKAIIQPGGSIKDQDSIDMANKYDLAMVFTDVRH
                     FRH"
     gene            8647..9921
                     /gene="purD"
                     /locus_tag="IV334_001456"
     CDS             8647..9921
                     /gene="purD"
                     /locus_tag="IV334_001456"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HAP8943530.1"
                     /translation="MNILVIGAGGREHAISKKLLASPKVDTVYCAPGNPGMTKDGINC
                     VAISERDHSALIHFVKKEAIDWSIVGPEVPLLNGIVDDFMAEGLKIFGPTKAAALIEG
                     SKSFAKQLMDTYQIPTAKSRSFECYEKAAAYIKENGAPIVVKADGLAAGKGVVVATTV
                     KEALDAAEQMLNQHRFGDSSKKIVVEEFLAGEEFSLLAFVRNTKVYPMVISQDHKRAF
                     DNDKGPNTGGMGAYSPVPQISKKMIEKAVEEVLKPAALGMKELGRSFTGVLYAGLIAT
                     KEGPKVIEFNARFGDPETQVVLSRLKSDFAQVIDDLLENRTVELKWQQEGYNVGVVVA
                     AAGYPEEYETGMVLPDFLEEELSVYYAGVSSNNGKLVGAGGRLYLVEAYGETIKEAQK
                     KIYTALMDKTTDGTFYRTDIGEKAANSELIVK"
     gene            10043..10957
                     /locus_tag="IV334_001457"
     CDS             10043..10957
                     /locus_tag="IV334_001457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase family oxidoreductase"
                     /protein_id="HAP8943531.1"
                     /translation="MKKIQLGTSDQKAAAVILGCMRINNAEDPVKVIETAYENGIDFF
                     DHADIYGNGECEEIFADALAKTSIKREDLFIQTKCGIVPGVMFDFSKKHIIESVEKSL
                     TRLKMDYVDALLLHRPDTLVEPEEVAEAFDELERSGKVRYFGVSNQKPMQIELLKKYV
                     RQPLVANQLQFGLKHTGMIDQGIHVNMTDEASIDHDGSVLDYSRLHDMTIQAWSPYQY
                     GFFEGVFIGNEKFPELNQTLEELAAKYQTTPTGLASAWILRHPANMQVIAGTMNVGRI
                     KEIAQASEINMSREDWYELYRAAGNILP"
     gene            complement(11090..12655)
                     /locus_tag="IV334_001458"
     CDS             complement(11090..12655)
                     /locus_tag="IV334_001458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HAP8943532.1"
                     /translation="MYKVFIIEDEYLIRNNLREQITVLGSYYPITYVGEAGDGEMALS
                     SIIDLQPDIILTDIQMPFMDGLTFSKEARKLLPWTRLIFISGFDDFDYMKGAIQVQAD
                     DYLLKPIKSSELKQAIEKAVTALDLQQKETHTSSDLVFELKKNHFLNGLFKGSLTISE
                     TLKLAESFNRSLAGKKMTVLLAANQVSTDFEDYAHLSDYLKFLFRENQQVIFSAVSSR
                     FIKFLIVDSLEENVIETSYQVAQTLVHELEKGQKNNFSVAIGPVAERISELPASFEIT
                     QMILETASSLKEQIISYEDLIQEAKLPVDSVFYFDLSLDLSSLSKNEVSKYIEHLTKP
                     QGTPTRTRLYRLFVLTKLLQHVCLKEKKIPQEMVHCTSTSQRLAISADDSLYRSTVYM
                     FISFLTALPSQPSRNKYQPILNHALDFIKENFTDPDMSLTWVAQQVALSPSHFSTIFS
                     QSFNQTFIDYLTEQRIELAKKLLQQSDYRISDIAFEIGYNDPNYFSYLFKKKQGISPK
                     EYRTQLFLKNKNN"
     gene            complement(12648..14099)
                     /locus_tag="IV334_001459"
     CDS             complement(12648..14099)
                     /locus_tag="IV334_001459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HAP8943533.1"
                     /translation="MTKNLSIKQIFAQANKAMLLFAVIPLLLSILLYSQQLLIYQQTI
                     ANIQKADAIALKVDQKVLESMWDLISGQVTVDEFNEENILEELRNDIREIKNNTNTLK
                     EISTLDVSLRTIDTLEDYQNELINNILAKDFMDTNEEIINQVDSVTQLLSDILQDFVR
                     VEIDLASKKNTTLVRSLILLTIVELGVLSTIFFYAKKNRRFLERKVQEPINQLIEMSE
                     QLSSGNLGFRLTLPETLELQTLTASMNKMAEDLNQSLRENALKQYHLAQSEVRVLQAQ
                     ITPHFIYNSLDAIISLIEQDCFEEALTMTHALSGFFRTSLSKGQDWIPLEKEISHIND
                     YLVILKIRYGHMLDYKIDIPETLFPVSILKMILQPLIENAVYHGTKYVRHKGFIRITA
                     CKIDEMIQFKVIDNGIGIPAEKLAEIRKELAKGIDSDFSIGYGLYNVNKRLLLYYGND
                     QKPITIDSKYREGTTVTLMIPLSPKTIGGNKHV"
     gene            complement(14113..15129)
                     /locus_tag="IV334_001460"
     CDS             complement(14113..15129)
                     /locus_tag="IV334_001460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAP8943534.1"
                     /translation="MKRVLFFLALVVIAYQMMGCTPNTKEAPDPLVVGFSQSGTESSW
                     RKKHTESIITALEKEDYQVLYRNGYMNQERQIQDIRTFIAYKVDMIIFTPLQESGWDS
                     VLKEAKRAGIPVILVDRHIQTNDPELFVTHIGPSFKAEGNRAGLFVSNHFTNSKKQEI
                     RILELAGSENSTPTKLRSEGFLESINRKPTLKKDGTIDHKPPMTIVHRIVGDFIRMKG
                     KDQMKRYLETNDLSEIDVLYSHSDEMTHGALAAIKETSIKPGEDLIIVTIDGQEDMIE
                     KLRSGIVNCVVECKPDVGWYVANTVTRYFGNIQTGKTLPKDIFISETVFSQQNIANIP
                     TRNY"
     gene            15349..16437
                     /locus_tag="IV334_001461"
     CDS             15349..16437
                     /locus_tag="IV334_001461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HAP8943535.1"
                     /translation="MKNKFRKIVGTIAILTASILLSACGNSGSADDSTGYVGIAMPTK
                     SAERWIADGNNMVSELEKLGYKTDLQYGEDKVENQVAQIENMITKGVDTLVIASIDGS
                     ALTDVLAKAKEADIKVIAYDRLLMNSENVDYYATFDNFGVGVSQAAYIEEHLGLKEGK
                     GPFTIELFGGSPDDNNALINYNGVMSVLQPYMDNGQLVVPSGQTSFSQIATLRWDGST
                     AQARMDNLLSANYTDQTLDAVLSPYDPISLGIISSLKGVGYGSESKPLPVITGQDATV
                     AGVKSIIAGEQTQTIFKDTRILAKNTIEMIKAISDGEEVPVNDTETYDNGVKTVPTYL
                     ANTVSVDKDNYQAELIDTDYYKESDLKN"
     gene            16491..18017
                     /locus_tag="IV334_001462"
     CDS             16491..18017
                     /locus_tag="IV334_001462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HAP8943536.1"
                     /translation="MADCILKMADITKKFSGIRALDCVNLMIERGEIHALCGENGAGK
                     STLMNVLSGLYPYGSYDGEIFYDGKLCQFKDLKDSEEKGIVIIHQELALSPYLSIKEN
                     IFLGNEQAERGVIDWELTEKKTYELLNKVGLSIDPNLLVSQIGVGQQQLVEIAKALSK
                     SVRLLILDEPTAALNEEESANLLRLIRELKEQGVTSIIISHKLNEIVDIADRITILRD
                     GQTIETLENEMISEEHIIRGMVGRDLVDRYPERKPDIGEVYFKVEDWTVYHPLDHERI
                     INDHINLHLKKGEIVGIAGLMGAGRTEFAMSLFGHSYGSKISGRILKEGAEISVKTVP
                     SAIANGLAYVSEDRKSLGLNLLMDIRENVTLSSLDKISQKGVLDKEKEVTEAENFRKK
                     MRIKTNSIHQIVSSLSGGNQQKVVLSKWLMTQPDILFLDEPTRGIDVGAKYEIYTIIE
                     EMAANGKAVCLISSELPEILGMCDRIYTMNEGRITGEVSREDADQEVLMKLMTKEEVG
                     "
     gene            18019..19224
                     /locus_tag="IV334_001463"
     CDS             18019..19224
                     /locus_tag="IV334_001463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP8943537.1"
                     /translation="MEQAKNLTDKKEKKLNLKSRAADLFGRYSMVIILAGLLIAFQLM
                     TDGIFWRPLNITNLVLQNSHILVLAAGMLLVVLLGHVDLSVGSVMAFVGAIAGMMMVN
                     RQMNPWMTVIACLAVGGLIGAWQGFWVAYAGIPAFIVTLAGLLMFRGLTQVVLGGQSL
                     APFPAVFQKMSTGYLPDLSEGSSLHFLTLILGMIITIVLVVGQWRTRNRRKNHLFEVE
                     SPTAFLVKAVFTAVVVLGITYIFASYQGYPIILIILGLIVAAYGFLTNKTVAGRQIYA
                     TGGNRKAAELSGIKTKRITFWVFVNMGVMAALAGLVLAARLNAATPQAGTSMELDAMA
                     AVYFGGASTSGGIGTIMGAIVGGLVMGVLNNGMSILGVGVDWQQAIKGLILLLAVVLD
                     LYNKKKRTT"
BASE COUNT         6171 a         3280 c         4428 g         5397 t
ORIGIN      
        1 taaaacacga acattaaaag aatctaatgc cttaaatgta catatttttg ttgatttata
       61 aaaagatgat tgctaaaatt taaagtgaaa aaacgaacaa aaaacgatgt atttgctttt
      121 aatgtaaata aaaaggagag acgtacatgg agaagaaaga acttttgtat gaaggaaaag
      181 ccaaaaaaat atatcttact tctgatgaga acttgatttg gatagaatat ttagatcaag
      241 caactgcgtt gaatggcgcg aggaaagacc agataagtgg aaaaggagaa ttgaataatc
      301 aaatcacaag tgcaattttc cgttatctga ctcaaagagg aataaaaaat cattttattg
      361 aacaattgtc taaaaccgaa caattagcaa aaaaagtaca aattatccct ctggaagtgg
      421 ttatccgaaa tattgcagca ggaagtttct caaaacgtct aggtgttccc gaaggtaaag
      481 aattcatcaa accaatcatt gaattctact ataaggatga ccagttggat gatccattta
      541 tcaatgaaga gcatatctat ttcttacaac ttgcaaaacc ggaagaaatc agtgagctga
      601 aagcccaagc gttggctatt aatgaaattt tgaccgaatt atttgctaag gtcggtttgg
      661 ttttagtaga tttcaagcta gaatttggcc ggtcgaatgg acaaattttg cttgcggatg
      721 aagtaacacc ggatacttgc cgattatggg atgaacgtac gaatgaacat atggacaaag
      781 acgtttaccg cagagatcta ggtgatttga tcccagtcta tcaagaagta tataaccgtc
      841 tatcggaact aactgaagaa aaggagcgag tatgatgtat ttcgtaaaag tatatgtaac
      901 ctataaagat tcagtgttag atcctcaagg ggaggcagta aaaaaagcag tccatcgtat
      961 gggatatgaa acaatcgaag atatccgaat aggaaaatac tttgagatac aacttgctga
     1021 tgcagaagaa ccaatcgata cagtgattga aacgatttgt gacaagctgc ttgcaaatgt
     1081 aaatatggaa acataccgat atgaaatcaa aaaaatggag gaagtataac atgaaatttg
     1141 cggtcatcgt ttttccaggt tcaaattgtg acgcagatat gttatgggca gttcgcgaga
     1201 tcatgggcgc tgatgccgaa tttgtacggc atgatgagtc ctctttagaa ggatttgatg
     1261 gggttttact gccgggagga ttctcctatg gtgactattt gagatgcggt gcgattgctc
     1321 ggttttctac aatcatggaa gaagtgattc gttttgctga agaagggaaa ccagtattcg
     1381 gtacatgcaa tggattccag atattgacag aagcaggact tttgccaggt gtactgagac
     1441 gaaatgagtc tttgcatttt atttgcaaaa ctgtaccatt gaaagtcaac cccaagacgg
     1501 cctttacttc agagtatgaa gccgacgaaa taatctatct tccagttgct catggggaag
     1561 gaaattattt ctgtgacgaa gaaacacttc aagaactaaa aaaaaataat cagattgcct
     1621 ttacttataa tgacaacatc aacggcagcg tggaaaacat tgcagggatt acgaacaaag
     1681 cagggaatgt gttaggaatg atgcctcatc cagaacgtgc agtagaagac ttgctaggat
     1741 cagaagatgg gaaacggttc tttgcttcca ttttaaaaaa cttcggaaag gtgaccgtat
     1801 agatgaaaac attgaaagaa ccgacagctg aagagatcaa gaaaaaaaga atctatgccg
     1861 aatggggatt gactgatgaa gaatatcgaa tgatcgaaga agatatctta ggaagaatgc
     1921 ctaattatac ggaaactgga ctgttttctg ttatgtggag cgaacattgt tcctataaaa
     1981 attctaaacc ggttctacgc aaatttccta ctagtggacc tcatgtgttg caaggccctg
     2041 gagaaggcgc aggcatcgtc gacatcggtg atggactagc agttgtcttc aaagcagaaa
     2101 gtcacaatca tccttcagcc gtggagcctt atgaaggagc tgcgacagga gttggcggaa
     2161 tcatcagaga tattttcagc atgggggctc gtccaattgc gttgctagat tcattgaggt
     2221 tcggcgaatt agataatcca agaacgaaat atttactaga agaagtagtt gcaggtatcg
     2281 gcggatacgg caattgtatc ggcataccga ctgtaggcgg cgaaattgca tttgatcctt
     2341 gttatgaagg aaacccatta gtcaatgcaa tgtgcgtagg tttgattgaa cacaaacata
     2401 tccagaaagg gcaagccgct ggcgtcggta attcgatcat gtatgtaggt gcaaaaacag
     2461 gtcgcgacgg tatccacgga gcaacttttg cctcagaaga atttaatcaa gaagaagaac
     2521 agcaacgttc tgctgtacaa gtaggcgatc cttttatgga aaagctgctg cttgaagcgt
     2581 gtttggaatt aatcttgcat cattcggatt ctctaatagg tatacaggat atgggagcag
     2641 caggacttgt ttcatctagt gcggaaatgg cttcaaaagc tggctcagga cttatattga
     2701 cattagatga agtcccacaa agagaaactg ggatgacacc ttatgaaatg atgttatcgg
     2761 aatcacaaga acggatgttg atttgtgtta aatcaggaga ggaagaaaaa atccaagaac
     2821 tatttcagaa gtatgatcta gatgcagtca ctatcggtaa agtaacagac gacgggcaat
     2881 atcggctata tcatcaagga gaagaagtag caaatctgcc agtcgatgca ttagctgaag
     2941 atgcaccagt ttatcataaa gaaatgaaag aaccagctcg aatcgtagaa tttcagcagt
     3001 tagctcctta tcagccagtt atcgaagaac ctggacaagt attgcttgat ctgctgcagc
     3061 aaccgacgat tgcttcaaaa cgttcaatct acgaaactta tgattcacaa gtacaaacca
     3121 atacagttgt ccgtccagga agcgatgctg ctgtgatgag ggttagaggg acgaataaag
     3181 cgttagctat gaccacagac tgcaacgcgc gctatctgta tttggatcca aaaatcggag
     3241 gacaaatagc agtggcagaa gctgctagaa atattattgc tagcggtggt aagcccttgg
     3301 cgatcaccga ctgcctgaac tatggttctc ctgataaacc agaagttttt tgggaattat
     3361 cgacttcagc tgacggaatt gctgctgcgt gtgaaacgtt aggaactcct gttatttcag
     3421 gaaatgtctc tttatataat gaaacggatg gtcaagcaat ctatccaaca ccaatgattg
     3481 gtatggtcgg attgattgag gatcataaac atatcactac tcaggaattc aaaaaatcag
     3541 gggatctaat ctatatatta ggtaagacgt ttgcagattt tgatggcagt gaattacaaa
     3601 agatgcagct tggaagaatc gaaggtgtga tccgaaactt tgatctatcc attgaaaaga
     3661 gaaaccaaga gttagtattg acagccatcc aaaacggatt gattgaaagt gctcatgact
     3721 gttcagaagg tggcttggca attgcactgg cagaatcagc tttcaaacat cagctaggga
     3781 tatctgtaca gtttgaattg tcctctgctc aattatttgc agaaactcaa tcaagatttg
     3841 tgttgacagt cgcaccggaa aataagacac gattcgaaga aatgatggga gatgctgctg
     3901 ttttagcagg taaggtgacg gatgaagcga taatcgaaat ttcagcaact gacggtcaga
     3961 tcaaaataga aactgcagta gcaagaaagt gctgggagga tgcaatcgta tgtcttatga
     4021 agtgaagagt ttaaatgaag aatgcgggat cttcggtgtt tggggtcatc cagacgccgc
     4081 tagtgtggca tacttcgggc tccatagtct tcagcatcga ggacaagaag gtgcaggtat
     4141 cgtgtccaat gatcgaggaa agcttaaggg gcatcgagat ttggggctgc tttctgaggt
     4201 attcaaagat caaagaaaat tgcagcagtt aacaggagaa gctgcaatcg gacatgtacg
     4261 ttatgcaaca gcaggcaatg gaagtgtgga caatattcag ccttttctat tcaaatttca
     4321 tgatcagtcg atcggcttag cgcataatgg taacttgatc aatgctcgtt cccttagaaa
     4381 agagttagag gaaaatgggg cgatcttcca ttctaactcc gatacggaga tattgatgca
     4441 tttgatccga cgaagtgaag aaacagattt tttaaataaa ttaaaaagtg ctttgaatga
     4501 agtcaaaggt ggctttgcct atttattaat gacagaaacg gcaatgattg ccgctttgga
     4561 cccaaatggt tttcgtccat tagcaatcgg acaaatgaaa aacggagcat acgtgattgc
     4621 ctcggaaact tgtgcattag aagttattgg ggcacgtttt ataagagatg tccagcctgg
     4681 tgagatagtg atcatcaatg acgaaggaat ccagattgat tgctttacga aggacacgca
     4741 gctttctatt tgttcgatgg aatatatcta ttttgccaga ccagattcaa acatcgcagg
     4801 gatcaatgtg catactgcac ggaaaaatat gggacggaat ttagcgaaag aatcgccagt
     4861 aaaagcagat atggtcgttg gcgtacctaa ttcttctttg tcagccgcaa gcggttatgc
     4921 ggaagctagt ggaatccctt atgaaatcgg attagtaaaa aatcaataca ttgcccgaac
     4981 atttatccag ccaacgcctg agctaagaga gcaaggtgtt cgaatgaaat tatctgctgt
     5041 acgaggagta gtggaaggta aaaaggtgat tatggtcgat gattcaattg ttcggggtac
     5101 gacaagcaga cggatcgtcc atcttttgaa agaagcaggg gcaaaagaag tccatgtgag
     5161 gattgcttct cctcctttga aatatccatg tttttacggg attgatattc aaacgcgtaa
     5221 agaactcatt gcggcaaatc acacgattga tgaaatcaaa gaatgtatcg gtgcagattc
     5281 tttaggtttt ttaagtgaag agggtctgat cgaatcgatc ggtctggaaa aagatgctcc
     5341 gtattcagga ttgtgtatgg cttatttcaa cggagattat ccgacgccgt tatatgacta
     5401 tgaagaaaaa tatcaggaat cactaaaaga aaaagtatcg ttcttttaaa agcagaccaa
     5461 gaagggagta tatgaattca atggcaaatg catacgcaaa agctggcgta gatgtcgaag
     5521 ctggatatga agtagtagaa cggatcaaga agcatgtcaa aaaaacggaa cgtttaggta
     5581 tgatgggcgc tataggagga ttcggcggtt gttttgattt atcggtcttt caattaaaag
     5641 agccagtcct tgtctcgggg accgatggcg tggggacgaa attagcagtg gcaatcgaag
     5701 cagacaaaca cgatacaatc gggatcgact gcgtagcgat gtgtgtcaat gatatcttag
     5761 cacaaggcgc agagccgtta tatttcttag actacttagc aacggggaaa aatgatccgt
     5821 tacgtttgga aaatgtagta aaaggtgtag cagatggatg tgttcaagga aatatggcgc
     5881 tgattggcgg agaaacagct gaaatgcctg gtatgtactc aggtaatgat tatgatctag
     5941 ccggatttgc tgtagggata gctgaaaaat ctcaattatt gacaggagaa acgatcaacc
     6001 caggagatgt gttgcttgga atagcatcta gcgggattca ttcaaatggg tactctcttg
     6061 ttcgcaaaat tttctttgag cagcagcagt tttctttatc atcaaaactg ccagaattga
     6121 aaaaaacact tggcgaagaa ttgcttgagc caacgaaaat ctatgtttct tccttgcttc
     6181 cgctgatcaa agaaaagaaa ataaaaggag ctgcacatat cactggtgga gggtttgtag
     6241 aaaatatccc gcgaatgtta ccagaatcta tggctgcaca aatcaaactt ggctcatggc
     6301 cgattcttcc aatattcggc tgtttacaaa aatatggtga aattcctgag atggagatgt
     6361 atgaaatctt caatatgggc atcggtatgg tcctggcagt cgaagaatcg aaagtaaaag
     6421 aaatagtaca cgaaattgag caagctggcg aaaaagttta tgaaatcggg aaagtgatcg
     6481 ccaaagaaaa acatcgtgtg gtgtttatag gagaagaacg atgagaatcg ctgtttttgc
     6541 ttctggaaac ggaagcaact ttcaagcgct agccgattat ctatcgaaaa aaggattgga
     6601 atcttcgatt gactggttgt tttgtgatca gcctgaagca tatgttttga aacgtgcaac
     6661 tgcgttgagt gtaccggcag attgtttttc acctaaagaa tttgattcaa aaaaagagta
     6721 tgaagaagca attttgcata aattgaaaga aaaaaagatc gatttgatcg tattagctgg
     6781 atatatgcga atcattggac cggtattgct gaaaaattat gataaaagaa tcatcaatat
     6841 ccatccgtct cttttacctg ccttccctgg tttgcatggc atacgtgatg cctttgaagc
     6901 tggagtaaaa gaaacgggtg tcacaattca ttatatcgac caaggtgtag atactggacc
     6961 aatcatccgg caggaaaaag tgagaattga acaggaggac acgttcgatt ctttagaaga
     7021 aaaaatccat agagtagagc atcgaatcta tccagaagtg attagtgaaa ttattgaaaa
     7081 tggaggctat ctgaaatgac aagagcattg atcagtgtat cagataaaac tgggattgta
     7141 gcattcgccc aaggtttgat tgaacaagga gtagaaatca tttcaacagg aggaacaaag
     7201 catgcgttgg ataaagctgg gatcaaaacg ctttcgatcg aagaagtgac agggtttcct
     7261 gaaatgatgg atggccgtgt caaaacactt catcctaaaa tccatggtgg tcttttagga
     7321 agacgcgatc tccctagcca tatggaggca atggaaacgg aaaatatcca accaatcgac
     7381 tttgtttgtg tcaatttata tcctttcaaa gaaacgatta tgaaaccaga tgtcgaaata
     7441 gcagaagcta ttgaaaacat tgatatcggt ggtcctagta tgctccgtag tgcagcaaaa
     7501 aaccatgaat ttgtcacctc tgttgttgat ccatctgact atgaagaagt actttcagaa
     7561 ttacgtcagt atgggcatac ttcactttct ttcagacaaa aactagcggc aaaagtcttt
     7621 cgtcatactg cggcatatga tgcactgatc gctcaatact taacggattg ggtggaagaa
     7681 aaagaaccgg aaaaattgac acttacgtat gaacgaaaac aaacgcttcg ttacggtgaa
     7741 aatagccatc aagccgctgc cttttatcag tccgctcttc tggaaaaata ttcgatcgca
     7801 gcagcaaaac aactccatgg gaaagaactt tcttataata atatccgaga tgcagatgct
     7861 gcgctacgga tcatgaagga atatgacgag ccaacggtag tcgccttgaa acacatgaat
     7921 ccttgtggta ttgggacagc atctacgatt ttagaagcgt gggaagcagc gtatgaagca
     7981 gatcctgttt caatctttgg tgggatcatc gttttgaatc gagaagtaga tctgccaaca
     8041 gcagaacaga tgcataagct gtttctagaa atcatcattg ccccttctta tacacaagaa
     8101 gctttggaat tactgcaaaa aaagaaaaac atccgattac tgacagtaga ctttgcgcag
     8161 gaagaaaatc aaaatcgaga agaaacggtc tcagtcttag gtggacttct tgtacaagat
     8221 caagatctgg caatagaaaa tgaagaagag tggaaagtcg tgacaaaacg taagccgact
     8281 ccagcagaag caaaagcatt agcgtttgct tggagagctg tcaaacatgt gaaatctaat
     8341 gcgatcgtac tggcaaacga acgtcagaca gtaggaatcg gagccgggca gatgaatcga
     8401 gtcggttcag taaaaattgc gattgaacaa gcgcaagaga aaatggaagg tgcagtttta
     8461 gctagcgatg cttattttcc aatggatgat tcggttgaat atgcagccaa acatggtata
     8521 aaagcaatca ttcagccagg aggaagcatc aaagaccaag attctattga tatggcgaat
     8581 aaatacgatc tagcaatggt ctttactgat gttcggcatt ttagacatta gagaggagtc
     8641 ggataaatga acattttggt gatcggtgca ggtggtcgtg aacatgcaat aagtaaaaaa
     8701 cttttagcca gtccgaaggt ggatacggtg tattgtgcgc caggaaatcc ggggatgaca
     8761 aaagatggta tcaattgtgt cgctatcagt gagcgagatc attcagcgtt aatccatttt
     8821 gttaagaaag aggctattga ttggagtatc gttggtcctg aagttccttt gttaaatggg
     8881 atcgtcgatg attttatggc ggaaggattg aaaatcttcg gtccgacaaa agcagcagct
     8941 ctgatcgaag gttcgaaaag ttttgctaaa caattaatgg atacttatca aatcccgaca
     9001 gctaaatcca gaagttttga atgttacgaa aaagcagcag cgtatatcaa agaaaatggt
     9061 gcgccaatcg tagtgaaagc ggacggctta gcagctggga aaggtgtagt cgtggctacc
     9121 acagtgaagg aagcgctgga tgcggcagaa caaatgttga accagcatcg atttggggat
     9181 agtagcaaaa aaatcgtcgt agaagagttt ttggcaggag aagaattctc ccttttggcg
     9241 tttgtccgta ataccaaggt gtatccgatg gtcatttcgc aagatcataa gcgggcattt
     9301 gataacgata aagggccaaa tacaggcgga atgggtgctt attcaccagt accgcaaatt
     9361 tcaaaaaaga tgattgaaaa agctgtggaa gaagttttaa aaccagcggc tttgggaatg
     9421 aaagaactag gacgctcttt cacgggtgtt ttatatgcgg gtttgattgc tacgaaggaa
     9481 ggaccaaaag tgatcgaatt caatgcgcgt ttcggtgatc ctgaaacgca agtcgttctt
     9541 tctcggttga agagtgactt tgcgcaagta atcgatgatt tattagagaa tcgtacagta
     9601 gagttaaagt ggcagcaaga aggatacaat gtaggcgttg tggttgcagc tgccggttac
     9661 cccgaagaat atgagacagg aatggttcta ccagattttc tggaagaaga gctttctgtt
     9721 tactatgctg gagtttcctc aaataacggc aaactagttg gagcaggagg acgtctttat
     9781 ttagtagaag catacggtga gacgatcaaa gaagcacaga aaaaaattta caccgcgtta
     9841 atggataaaa cgacagatgg aacattttat cgtacagata ttggagaaaa agctgccaat
     9901 agcgaactga tcgtgaagta aatcagctgc ttccatttat aaaaaataca acaaacataa
     9961 tcggaagcgc cgattttagt gaagcctttt cataaagagt gtggtacact tttgttgtgt
    10021 atataaataa ggaggagttc agatgaaaaa aattcaatta ggtacttctg atcagaaagc
    10081 agctgcagtg atcttaggct gtatgcgtat caacaatgcg gaagatccag taaaagtgat
    10141 tgagacagct tatgaaaacg gcattgattt ttttgaccat gcagatattt atggaaacgg
    10201 tgaatgtgaa gaaatctttg cagatgctct agcaaaaaca tcaatcaaac gagaagatct
    10261 ttttattcaa acaaaatgtg gaatcgttcc aggtgtgatg tttgattttt ctaaaaaaca
    10321 tatcattgaa tctgtagaaa aaagtttgac ccgtctgaaa atggattatg tggatgcttt
    10381 acttcttcat cgtccagata cattggtaga accagaggaa gtagctgaag cgtttgatga
    10441 attggaacgg tcaggaaaag ttcgttattt tggcgtcagc aatcaaaaac caatgcaaat
    10501 cgaattgctg aaaaaatatg ttcgtcaacc attagtagct aatcagctgc aatttggctt
    10561 gaaacatact ggaatgatcg atcaagggat tcatgtcaat atgacagatg aagcaagtat
    10621 cgatcatgac ggtagtgtac ttgattattc ccgattgcat gatatgacga tccaagcatg
    10681 gtcaccatac cagtatggat tttttgaagg tgtatttatc gggaacgaaa aattccctga
    10741 attaaaccaa acattagaag aactagcagc taaatatcaa acgacaccaa ctggcttggc
    10801 ttctgcttgg attttacgcc atccagccaa tatgcaagtg atcgccggta cgatgaacgt
    10861 agggcgaatc aaggaaatcg ctcaagcttc agaaattaat atgtctcggg aagattggta
    10921 cgaattgtac cgtgctgctg gaaacatttt accttaaaga ttgtcataag caggtcaaaa
    10981 gggctataaa ttaaaaagac agaataactt gaaagctgtt ttcagcttcg agatattctg
    11041 tcttttttta ttgcaaatgg aaagagtatc attgtacgga tttcgtttat tagttatttt
    11101 tgtttttaag gaaaagctgt gtccgatatt cttttggaga aatcccttgt tttttcttaa
    11161 ataaataact gaaatagtta gggtcattat aaccaatttc aaaagcaata tctgaaatac
    11221 gatagtctga ttgctgcaat aatttttttg ctaattcgat tctttgttct gtcagataat
    11281 cgatgaatgt ttggttaaac gattgagaaa aaatcgtact gaaatgagag ggacttaatg
    11341 caacttgttg agcgacccaa gttaaggaca tatctggatc ggtaaaattt tctttgataa
    11401 aatccaatgc atggttcaaa attggctgat atttgttcct tgaaggctgt gagggcagag
    11461 cagttaagaa agaaatgaac atataaactg tcgaacggta aagcgagtcg tcggcgctga
    11521 ttgctaagcg ttgactggtg gatgtgcaat gaaccatttc ttgcgggatt ttcttttctt
    11581 tcaggcagac atgctggagt agcttagtca agacaaataa acgatataat cgtgttcgag
    11641 ttggtgtgcc ttgtggtttc gtcaagtgtt cgatgtactt agatacttcg tttttcgata
    11701 atgaagacaa atcaagtgag aggtcaaagt aaaagacaga atcaactgga agtttcgctt
    11761 cttgaatcaa atcctcatag ctgataattt gttctttaag ggaactggcg gtttcaagta
    11821 tcatctgcgt gatctcaaaa gatgcaggta attcgctgat tctttctgcg acaggaccaa
    11881 tggcgacaga aaaattattt ttttgtccct tttctaattc atggaccaac gtttgggcta
    11941 cctgataact ggtctcgatc acattttctt caagagaatc aactattaaa aatttgatga
    12001 atcgagagga aacagcagag aagatcactt gttgattctc cctaaataaa aacttcaaat
    12061 aatcactgag atgagcgtaa tcttcaaagt ctgtactgac ttggtttgct gctaataata
    12121 cggtcatttt tttccctgct aaagagcgat tgaaagattc agcaagtttc aatgtttcac
    12181 tgatcgtaag tgagcctttg aacaaaccgt tcaagaaatg attttttttc aattcgaaaa
    12241 caagatcaga agaagtgtgc gtttcttttt gctgcaaatc taaagcagtt actgcttttt
    12301 ctattgcttg tttcaattcg ctacttttaa tcggtttaag cagataatca tctgcctgta
    12361 cctggattgc tcctttcata taatcgaaat cgtcgaatcc actaataaat atgagacgag
    12421 tccaaggaag tagtttcctt gcttcttttg aaaaagtcaa cccgtccatg aaaggcattt
    12481 ggatatcagt cagaatgatg tcgggctgta agtcgataat tgaagacagt gccatctcac
    12541 catctccagc ttcgccgaca taagtgattg gataataaga accaaggaca gttatttgtt
    12601 cccgcaagtt attgcgaatg aggtattcgt cttcgatgat aaaaacttta tacatgtttg
    12661 tttcctccaa ttgttttggg agatagaggg atcatcagtg tgacagttgt tccttctcgg
    12721 tatttgctgt caatcgtgat aggtttttga tcatttccgt aataaagaag gagacgcttg
    12781 tttacgttgt aaagtccgta gccgatactg aaatctgaat cgatcccttt ggctaactct
    12841 ttacgtattt ctgccagttt ttctgcaggg atgccaatcc cgttgtctat aactttgaat
    12901 tggatcatct catcaatttt gcatgcggta atacgaataa atcctttgtg acggacgtac
    12961 ttcgttccat ggtatacagc attttcaatc aatggctgca agatcatttt taaaatagaa
    13021 acaggaaaaa gtgtttctgg tatatcgatc ttatagtcaa gcatatggcc gtatcttatt
    13081 tttaagatga ctagataatc attgatatgg ctaatttctt tttcaagagg aatccagtct
    13141 tgtcctttgc ttaaagacgt acgaaaaaag ccggaaagtg catgggtcat agtcagagct
    13201 tcttcaaagc agtcttgttc gatcaaagaa ataatggcat ccaaactgtt ataaataaaa
    13261 tggggagtaa tctgagcttg caatacacga acttcacttt gtgctaaatg gtattgtttc
    13321 aaagcgttct cccgtaaaga ctggtttaga tcttccgcca ttttattcat gctagcggta
    13381 agtgtttgta attccagtgt ttccggcagg gttaaacgaa agccaaggtt ccctgaagaa
    13441 agttgttctg acatctcgat cagctgattg atcggttctt ggacctttct ctctaaaaag
    13501 cgacgattct ttttcgcata gaaaaaaatg gtactaagaa ctccaagttc cactattgtt
    13561 aatagaatga gcgagcggac aagtgtcgta ttttttttgc ttgctaagtc gatctccaca
    13621 cgaacaaaat cttgtaagat atctgacagc agttgagtga cagaatctac ctgattgatg
    13681 atctcttcat tggtatccat aaaatctttt gccaagatat tattgatcag ctcgttttga
    13741 taatcttcca gtgtatcgat cgtacgtagc gatacatcta acgtactaat ttctttaagt
    13801 gtatttgtat tatttttgat ctctcgaata tcattcctaa gttcttctag gatattttcc
    13861 tcattgaact catctacggt tacttggcca gaaatcaagt cccacatact ttccaaaact
    13921 ttttgatcaa ccttcaaagc aattgcatct gctttttgga tatttgcgat cgtctgttga
    13981 taaatcaaaa gctgttgcga atataacagt atgctcagta gcaaagggat aacagcaaat
    14041 agaagcatgg ctttgtttgc ttgggcaaaa atctgtttta tgcttaaatt ttttgtcatg
    14101 atacttctcc agtcaataat ttctggttgg gatgttagct atattctgct gggaaaagac
    14161 agtctcggaa ataaaaatat ctttaggtaa tgtttttcct gtttgaatat ttccaaaata
    14221 acgagtaacg gtattggcta cataccagcc gacatcaggt ttacactcga ctacacaatt
    14281 gacgattcct gagcgtagct tctcgatcat atcttcttgt ccatcaatag ttacaatgat
    14341 caaatcttct ccaggtttga ttgatgtttc ttttatcgca gctaaagcac cgtgagtcat
    14401 ttcatcacta tggctgtata atacatcgat ttcggataaa tcatttgttt ctaaatatcg
    14461 tttcatctgg tcttttcctt tcatccgaat aaaatcaccg acaattcggt gaacaatagt
    14521 catcggaggt ttatggtcga tggttccatc tttttttagg gttggtttac gattgattga
    14581 ttccaaaaat ccttcggagc gtagcttggt cggtgtggag ttctctgaac cagcaagttc
    14641 taggattctg atttcctgtt tctttgaatt tgtgaagtga ttgctgacaa ataaacccgc
    14701 ccgatttcct tctgctttga aactggggcc aatgtgggtg acgaaaagct cgggatcatt
    14761 cgtttgaata tgtctgtcga caagtatgac agggatccct gctcgctttg cttcttttaa
    14821 tacagagtcc cacccggatt cttgcaaagg agtaaaaatg atcatatcca ctttataggc
    14881 aatgaacgtc cgtatatctt ggatttgccg ttcctgattc atgtagccat ttcggtagag
    14941 aacttggtaa tcttcttttt caagcgcagt gataattgat tcagtatgct ttttccgcca
    15001 gctactctcc gttcctgatt gcgaaaaacc aacgactagt gggtcaggtg cttcctttgt
    15061 attaggtgta caccccatca tttggtaagc gattacaaca agggctaaaa aaaataaaac
    15121 acgtttcact ttaaatcact ccatctggat ttgttagtac tatcttatca gataaaaggg
    15181 aaaatcaaag tcaaaaggaa tcaatcgaaa aaaatccaag aaacttaaaa aaatccaaga
    15241 caaaaaaagt acacctaaaa aatttaaagt aaatcccata aaaaaatcaa tgttaacgct
    15301 ttctttatta gctatactcg gtgtatcaat tgaaatgggg gataaaaaat gaaaaacaaa
    15361 tttagaaaga ttgttgggac tatcgctatt ttgaccgcaa gcattctatt aagtgcgtgt
    15421 ggcaattcag gatctgcaga tgatagtacg gggtatgtag gaatcgctat gccgaccaaa
    15481 tcagcagaaa gatggatcgc tgatgggaac aacatggtct cggaattaga aaaactgggt
    15541 tataaaacag atcttcaata tggagaagat aaagtagaaa atcaagtagc acaaatagaa
    15601 aatatgatta caaaaggggt agatacactt gtgattgctt ctatcgatgg ttcggcttta
    15661 actgatgtgt tagctaaagc aaaagaagca gatatcaagg tgatcgccta tgatcgttta
    15721 ctgatgaatt cagaaaatgt cgattattac gcgacttttg ataattttgg agtaggtgta
    15781 tcgcaagcag cttatattga agagcactta ggattgaaag aaggaaaagg accattcacg
    15841 attgaattat ttggtggctc gcctgacgac aacaacgcac taatcaatta caacggagtg
    15901 atgtctgttt tacaaccata tatggacaac ggtcagttgg tcgtgccatc tggacagacg
    15961 agtttcagtc aaatcgctac gcttcgctgg gatggttcta cggcgcaagc aaggatggat
    16021 aatctgttaa gcgcaaatta tactgaccaa acattggatg ctgttttgtc gccatatgat
    16081 ccaataagtt taggaatcat ttcgtcatta aaaggcgtgg ggtatgggag tgaaagtaaa
    16141 cctttacctg tgattactgg acaagatgcg acagttgctg gcgttaaatc gatcattgcc
    16201 ggggaacaaa cacaaacgat ctttaaagac acacgtatct tagcaaaaaa tacgattgaa
    16261 atgatcaaag caatcagtga tggagaagag gttccggtca atgatactga aacttatgat
    16321 aatggggtga aaactgtccc tacgtattta gcaaatacag tatctgtcga taaagataat
    16381 tatcaagcag agctgatcga caccgattat tataaagaat ccgatctgaa aaattaagag
    16441 gaaccaaaca aggaactaaa gagatctaag tcagagagag gataagaatt atggcggatt
    16501 gtattttaaa aatggcagat atcaccaaaa aattttctgg aatccgagcg ttagattgcg
    16561 tgaatctgat gattgaacgt ggggaaatcc atgcattgtg cggagaaaac ggagcgggaa
    16621 aatcaacatt gatgaatgtc ttgagcggct tgtatccata tggctcctat gacggggaaa
    16681 tcttttatga tggaaagctg tgtcaattta aagatttgaa agatagtgaa gaaaaaggca
    16741 tcgtcatcat tcatcaagaa ttagcactaa gtccttatct ttcaataaaa gagaatattt
    16801 ttctcggaaa tgaacaagca gaaagaggcg tgattgattg ggaactgaca gaaaagaaaa
    16861 catatgaact gctgaataaa gtcggtttgt ctatcgaccc gaatcttcta gtatcccaga
    16921 ttggcgtagg gcaacagcaa ctagttgaaa tagctaaagc attatcaaaa tccgtccgtc
    16981 tgttgatttt ggatgagcct accgctgcat tgaatgaaga agaaagcgcg aatttgctga
    17041 gattgatccg agagttgaaa gagcaaggtg taacctccat cattatttca cataagctga
    17101 atgaaatcgt ggatatcgca gatcgaatca ccattttacg ggatggacag acgattgaga
    17161 cactagaaaa tgaaatgatc agcgaagagc atattattcg cgggatggtg ggcagagatt
    17221 tagttgatcg ttatcctgaa agaaaaccgg atattggaga agtttatttt aaagtagaag
    17281 actggacggt ctatcaccct cttgatcacg agcgaatcat aaacgaccac atcaacttgc
    17341 atttgaaaaa aggagagatt gtaggaatag ctggactgat gggagcgggc agaactgaat
    17401 tcgctatgag tctatttgga cattcttatg gaagcaagat atccggacgt atattaaagg
    17461 aaggggcaga gatatcagtt aagaccgttc cttcagccat tgcaaacgga ctcgcttatg
    17521 tctcggagga ccgaaaatct ttaggactga atctgctgat ggatattcgg gaaaacgtga
    17581 cgctgagcag tttggataaa ataagtcaaa aaggagtatt agataaagaa aaagaagtaa
    17641 ctgaggcaga aaatttccgg aaaaagatgc gtataaaaac aaactccatc catcaaattg
    17701 tcagtagtct tagtggtggg aatcaacaaa aagtcgtttt atccaagtgg ctgatgacac
    17761 aacccgatat tctttttttg gatgagccaa cccgtgggat agatgttggg gcaaaatatg
    17821 agatctacac gattatcgaa gaaatggcag caaatggcaa agctgtctgt cttatctctt
    17881 ctgaacttcc tgaaatatta gggatgtgtg atcgaatcta tacgatgaac gaaggacgta
    17941 ttacaggaga agtctcgcga gaagatgcag atcaggaagt cttgatgaaa ttaatgacga
    18001 aagaagaggt gggttaagtt ggaacaggca aaaaatctta cggataaaaa agagaaaaaa
    18061 ctaaatttga aaagtagagc agctgatctt tttggtcgct acagcatggt gatcatttta
    18121 gcgggactac ttattgcgtt tcaattgatg acggatggta ttttctggcg tccattgaat
    18181 ataactaacc ttgttctgca aaatagccat atattagtac tagctgcagg gatgttatta
    18241 gttgtgctct taggtcatgt ggatttgtcg gtagggtcag tgatggcgtt tgttggtgca
    18301 attgcgggca tgatgatggt caatcgccaa atgaacccgt ggatgacagt tatcgcatgt
    18361 cttgcagtag gcgggctgat cggcgcatgg caaggttttt gggtagctta tgcagggata
    18421 ccggcgttta tcgtgacttt agcaggattg ctgatgtttc gtggactgac acaagttgtt
    18481 ctaggaggcc aatctctagc accttttcca gctgttttcc aaaaaatgtc tacgggttat
    18541 ttaccagact taagcgaagg aagcagcctt cacttcttga cattgatttt aggaatgatc
    18601 atcacgatcg tgttagttgt tggtcaatgg cgaacaagaa atcgtcggaa aaatcattta
    18661 tttgaagtgg aatctccaac agctttcttg gtaaaggcgg tatttacagc agtcgttgta
    18721 ctaggaataa cttatatctt tgcctcttat caagggtatc cgattatttt gatcattctt
    18781 ggtttgatcg ttgcagcata tgggtttttg acaaataaaa cagtagcagg tcgacagatt
    18841 tatgcgacgg gcggaaatcg aaaagcggct gaactatcag ggatcaaaac aaagcgaatc
    18901 acgttctggg tattcgtcaa catgggagtc atggcagctt tagctggatt agttttagct
    18961 gctcgattga atgcggcaac acctcaagca ggtacatcga tggaactgga tgcaatggcg
    19021 gcagtatatt ttggtggtgc ttcgacttcc ggcggcattg gaacaatcat gggtgcgatt
    19081 gtcggtggct tggtcatggg tgtattgaac aatggcatgt cgattttagg cgtcggggta
    19141 gactggcagc aggcaatcaa aggattgatc ttgcttttag ccgttgtgct agacttgtat
    19201 aataagaaaa aacgaacaac gtaggagaac gccggttatt cagaaaaaag actgtgacaa
    19261 gacatttgtc acagtc
//
LOCUS       DACDKM010000044        19130 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20834_2016
            SAMN12640370-rid9891663.denovo.044, whole genome shotgun sequence.
ACCESSION   DACDKM010000044 DACDKM010000000
VERSION     DACDKM010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640370
            Sequence Read Archive: SRR10040891
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19130)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19130)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742835.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742835.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:04:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,012
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 29 of 75
            Pseudo Genes (incomplete)         :: 33 of 75
            Pseudo Genes (internal stop)      :: 19 of 75
            Pseudo Genes (multiple problems)  :: 5 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19130
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640370-rid9891663.denovo.044"
                     /isolate="E20834_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(<1..703)
                     /locus_tag="IV334_001464"
     CDS             complement(<1..703)
                     /locus_tag="IV334_001464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP8943538.1"
                     /translation="MFWHFYKYRTKILLRNKSMLFWTLAFPILLGLMFMAAFGEIDQG
                     GNLETINTGIVIKADQTALSENFTTVLEQVEVNDKPLFEVQELSEVEANEKLNADDLA
                     GFYLIDGSSVSLHVGQAGMSQTLMKNVMDQFLQRIGIISSKVAEVENVDLAPIMTVFE
                     QEVSFQEVHAGRNMSFKSFYFFTMIGMAILYGTMWGVRNVQDQQANQSSNGIRLSLIP
                     RKQSLVSIANLAASFT"
     gene            complement(717..1641)
                     /locus_tag="IV334_001465"
                     /pseudo
     CDS             complement(717..1641)
                     /locus_tag="IV334_001465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015509966.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
     gene            1758..2837
                     /locus_tag="IV334_001466"
     CDS             1758..2837
                     /locus_tag="IV334_001466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HAP8943539.1"
                     /translation="MHKSRLLNETSFLLVISLLLLVSQGFVAMQVIYLLIAVLFTGVS
                     YLLPTKPNWIFQVILFLLCAFLPDLVLFLPATMRVIAPEESHLDYLFLLGFLLISTVG
                     NHPPIKVAFVVILLFFSFYLRKKDQRTTDLEKKYIVSKDTHWEKQQELSEQNKALALS
                     QETQLALQIVEERNRIARDIHDNVGHLLSSAILQIGALEMINQEPKLKEPLENLSATV
                     HTGMDRIRQSVHDLHETSISFQHSLEFLIADFPSSVTIEGNLFEGLNETQQNCFLMVI
                     KEALTNSIKHSHAKTVVLHFRTLPAFYRLQITNDGTSPQKNSAPGIGLKTMRQRVQEQ
                     KGQVHIFQENQQFQLTIILPREGHQ"
     gene            2869..3462
                     /locus_tag="IV334_001467"
     CDS             2869..3462
                     /locus_tag="IV334_001467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP8943540.1"
                     /translation="MTKSLQTILESTKEIRVLGIGHCAKDALVLYETHRPDVLLTDIR
                     MPKQTGIDAAKEILAKFPKAILLLLTTFKDEEYIREAFSIGVKGYLIKQNLQAIIPSV
                     KSAYNGQAVFGNEIIETFTQMMKNKPTIDSHAYASFSERELAIIKEVAAGKNNKEIAD
                     ALYLSDGTVRNYISQLLEKLDLRDRTQLAIYFYQHLQ"
     gene            complement(3572..3799)
                     /locus_tag="IV334_001468"
     CDS             complement(3572..3799)
                     /locus_tag="IV334_001468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005229738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HAP8943541.1"
                     /translation="MAKETEDKLAKLAKESGNDFKKLDEMEKEFKLNESPDVERDDRE
                     ESKLEKTLDKAPIFPPNTPNNPTNPGSPANP"
     gene            complement(3902..6490)
                     /locus_tag="IV334_001469"
     CDS             complement(3902..6490)
                     /locus_tag="IV334_001469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RecD-like DNA helicase"
                     /protein_id="HAP8943542.1"
                     /translation="MQPEEMPYFVGTVAAIFFQNPSNFYKVLLVHISETTADYREKEI
                     VVTGSFGDIQENETYRFYGELVDHPKYGRQLKLERYEQEKPTTANGIVAYLSSEKFPG
                     IGKKTAEKIVELLGEDAIEKIIADPSVLKQISGLTKPKREMLAETIRLNHGMDQVIMG
                     LNRYGFGSQLAFSIYQAYKNESLDIIEENPYQLVEDIEGIGFKRADNLAEQIGIAADS
                     PRRIRAAILHQIFQHAVQTGDTYVPAETLLQQTIRILETSRPIEIQPDQVATVIIQLV
                     EEGKIQQEETNLYENSLYFSEWGIGNSIQRLLSRKKEIHYPEKEVQKNIRRIEKIFGI
                     QYGPSQEQAIAEAIRSPLFILTGGPGTGKTTVINGIVNLFAELNGLELDPSKYTQEIF
                     PILLAAPTGRAAKRMNETTGLPASTIHRLLGLNGREKTPSVSTKELEGGLLIVDEMSM
                     VDTWLANTLFKAIPTNMQVILVGDKDQLPSVGPGQVLHDLLEIEQIPKMELNQIYRQG
                     DGSSIIPLAHEIKEGRLPKDFTKNQKDRSYFRSDAYQIEPLISKIVEKAKNKGFRAQD
                     IQVLAPMYRGPAGIDALNKMMQEIFNPGKPGKKEVHWNDTVYRIGDKVLQLVNTPELN
                     VFNGDMGEITGIILAKDSEDKVDELVIQFDANEVTYKRNEWNKITLSYCCSIHKSQGS
                     EFKMVILPMVHQYQRMLQRNLLYTAVTRSKELLILLGEEQAYVTCVANESASRMTTLK
                     SRIMDNDKMNITLRTKLAAYEDSLVGNDPFEDGNYTKEPRVVKEKLPVEDDSGTNETK
                     NQVMETVEPDLFSSFVGEEKKEEVEPAVSKDFVLTAEVIDNQLVDPMIGMENSSPYDF
                     IPAKNA"
     gene            complement(6510..7169)
                     /locus_tag="IV334_001470"
     CDS             complement(6510..7169)
                     /locus_tag="IV334_001470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HAP8943543.1"
                     /translation="MELYFTRHGKTQWNQERRFQGSNGDSPLLPQSYEEIKAFGKKVK
                     YVPFKAIYSSTAKRARDTAEGINKELARPVEIFYTDKLRELGLGTLEGQSIDEMYQKY
                     PDNLPNLRNHLDLYDPSPFHGETIESAITRIETVVADAVAQHKDGPLLFVGHGASLTA
                     AIQWMTGKELSELREMGGLFNSSLTILETEEPCRLLPYELKVWNQTDFLGTETAPEPL
                     L"
     gene            complement(7218..7721)
                     /gene="trmL"
                     /locus_tag="IV334_001471"
     CDS             complement(7218..7721)
                     /gene="trmL"
                     /locus_tag="IV334_001471"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (uridine(34)/cytosine(34)/5-
                     carboxymethylaminomethyluridine(34)-2'-O)-
                     methyltransferase TrmL"
                     /protein_id="HAP8943544.1"
                     /translation="MNHIVLFEPLIPANTGNIARTCAATNTPLHLIEPLGFSTDDKHL
                     KRAGLDYWNDVNITYHSNLEAFLTFLGNRKLHLISKFAHKVYSDENFADGEEHFFLFG
                     KETTGLPETFMRENEEKCLRIPMNDEHVRSLNLSNTAAMIVYEALRQQGFPNLELTHH
                     YENDKLD"
     gene            complement(7942..8808)
                     /locus_tag="IV334_001472"
     CDS             complement(7942..8808)
                     /locus_tag="IV334_001472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HAP8943545.1"
                     /translation="MLKKIDRGRQFLAENHQLFRCPVCQEEVKKSDNGLICKNNHRFD
                     LSKKGTLYFLSHAVKTEYDQGMFVPRRRMIQSGMYQPVIETIAQHLPEEVSLLDVGCG
                     EGSFLNELSKLKKIQNAVGFDISKEGVYAATEQSAEAFWCVADLTNLPFADQTFNAVL
                     NIFSPSHYQEFQRVLSEDGEVLKVVPQENYLKELRQAFYPNQPEKQSYSNERVVAKFA
                     EAMKLTERQRVTYEFEIPEENQKDLLAMSPLEWQVEERIKADLYKNPLKKITIDIELL
                     KGTKRKRFSSSF"
     gene            8917..9558
                     /locus_tag="IV334_001473"
     CDS             8917..9558
                     /locus_tag="IV334_001473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis protein CutC"
                     /protein_id="HAP8943546.1"
                     /translation="MIKEFCAENYTSIPLAIARGADRIELCDNLAVGGTTPSTGVIEE
                     TLSYAGEKDIPVMTIIRPRGGDFVYNDIELKIMHTDLIEAKKLGTDGVVFGCLTPSGW
                     LDEEALELLIETAEGLQITFHMAFDSIPEDRQFEAIDWLVEHGVDRILTHGGPADKTI
                     VENFPRLKELIAYTDGRLIILPGGGVTAENAAHVAKELNVSEVHGTKIVDLQL"
     gene            complement(9620..10981)
                     /gene="mgtE"
                     /locus_tag="IV334_001474"
     CDS             complement(9620..10981)
                     /gene="mgtE"
                     /locus_tag="IV334_001474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HAP8943547.1"
                     /translation="MDENQELEERFAQLTQDLRENNIQAFRNSFLEMHIYEQGQFYQS
                     LTEENRQLIYSYLSPKELADMFDVIEEDNEHMKEYLDEMRPSYAADMLSEMYTDNAVD
                     LLNTLDKKQIAKYLSLMSADDASEIKELLHYEDETAGAIMTTEFVSIVANQTVRSAMY
                     VLKNEADVAETIYYIYVVNQEGQLVGVISLRDLIVNDDDAMISDLMSERVLSVHVGDD
                     QEDVAQTFRDYDFLALPVTDYDDHLLGIVTVDDIIDVIDDEAASDYSGLAGVNVEEIN
                     ENPVKAASRRLPWLITLLFLGMSTASLISHYEDLVSEASILAVFISLITGTAGNAGTQ
                     SLAVAVRRLAISDEKDHGFVRLVLAEVLTGVVTGAVTGITIFLIVGFWKHNFILGFVI
                     GMAMLFAITVANLAGSLIPMLMDRLGFDPAVASGPFITTLSDLTSVLIYFNIASLFMG
                     YFM"
     gene            complement(10999..11898)
                     /locus_tag="IV334_001475"
     CDS             complement(10999..11898)
                     /locus_tag="IV334_001475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HAP8943548.1"
                     /translation="MQFTWDYEKDHSQQVKYFLKEKGISKGLLAKIKFQGGQIKVNDQ
                     VENVLFSLAKDDKVTIVIPAEGEHETVLLDETPIDIVYEDEHVLVVNKPAGVSSIPAQ
                     YHPNGTMANRVKAYYKKQGYENQVIHVVTRLDRDTSGLMLFAKHGFAHAKLDMQLREK
                     KFVKKYQALVSGRLSDLSAHGHIDLPIARDYSSLLKRMVSDEGRSAQTEYWIEKRLTE
                     IALVDIQLHTGRTHQIRVHFSAIGCALLGDDMYGGRMDLGIKRQALHCYDLRFYHPFT
                     EELLEFKQPLAADMEAVVRRFEE"
     gene            complement(11900..12697)
                     /locus_tag="IV334_001476"
     CDS             complement(11900..12697)
                     /locus_tag="IV334_001476"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD kinase"
                     /protein_id="HAP8943549.1"
                     /translation="MKVAIVHNKEAKTIEVTERLNALLEQAGIQRDDHEPELVISVGG
                     DGTLLSAFHHYSHCLNDVRFLGVHTGHLGFYTDWRDYELEELVDSLRTNREQSVSYPL
                     LDVRISYLDETPDQHFLALNESTIKRANRTMVADVYIKNELFESFRGDGLTISTPTGS
                     TAYNKSVGGAVIHPSINAFQLAEIASLNNRVFRTLGSPIVIAHDEWLEIKLENTEDYL
                     VTVDQLNVAKANIRSIYYRIAEERIHFASYRHMHFWHRVKDAFISED"
     gene            complement(12675..13379)
                     /locus_tag="IV334_001477"
     CDS             complement(12675..13379)
                     /locus_tag="IV334_001477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase family protein"
                     /protein_id="HAP8943550.1"
                     /translation="MERDWEAFLAPYEQTVSELKVKLRGIRKQFREQNRHVPIEFVTG
                     RVKPVDSIVTKSQLRHIPMGRLEEEMSDIAGLRIMCQFVEDIHQVVEIIRKRKDMKVL
                     QERDYITNKKASGYRSYHLVIEYPVQLISGEKKILAEIQIRTLAMNFWATIEHSLNYK
                     YQGVFPEEMSERLQRAAEAAYQLDEEMSSIREEIQEAQRLFSHGRGKSDDVYYRTLLK
                     KESEREEAGDESSDRS"
     gene            13510..14085
                     /locus_tag="IV334_001478"
     CDS             13510..14085
                     /locus_tag="IV334_001478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CYTH domain-containing protein"
                     /protein_id="HAP8943551.1"
                     /translation="MTQMLEIEYKSMLTKQEYEIFIAHYQLTDKDFRVQTNIYFDTVD
                     EQLKKQNCGLRIRFVGHQAEYTLKTPAEKGRLETTDTFITEEAEAFIFEKRLPRSGAV
                     FQKLSDLNIDPASLIQTGKLTTKRAEFPIEEGLLAIDESWGDNLHDFELELEVGDASV
                     GKIAFINFLKRFSLPYRPAKNKIQRMLEAKN"
     gene            14203..14880
                     /locus_tag="IV334_001479"
     CDS             14203..14880
                     /locus_tag="IV334_001479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HAP8943552.1"
                     /translation="MIEIYLFVNPLGEYCFEMEQQLLQFIEEEYGTPSKEKMQFRFLP
                     LVNLQTIGDVMQQKGISQNNLEERNHLFSMTYSAALDCKAAQFQGKKKGRRFLIKLQE
                     AVGCNDIPYSQELAESIFEEIGGDLNMFKEDRQSDFVKEVFFSDQKIAREMGITKHPS
                     AVVYNYACDRDFGVLVEVKGTSSLWRITELCKTTDNSYQVFNGEHLHTNDSQFHSRTS
                     HLHLLSN"
     gene            complement(14959..16767)
                     /gene="pepF"
                     /locus_tag="IV334_001480"
     CDS             complement(14959..16767)
                     /gene="pepF"
                     /locus_tag="IV334_001480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HAP8943553.1"
                     /translation="MEVKQLPKREELPENLTWDLTKIFSSDQEFDEKYLELSEELKQS
                     EKHKGTLDQGASQFLNAIEFVLRVYRQTEVIYVYAHLKNDQDTGNTDYQALYARASSL
                     FSKVSEAVSWFEPEILQLSDDQIWQYFKEEPKLEVYRHYIQQIVDNRAHVLSAEQESL
                     LAGAGEIFDASSDTFAVLNNADLVFPTIEGENGEIVQLSHGVYGQLLESTDRRVREAA
                     FKGLYSVYEQFRNTFASTLGTHIKGHNFKAKVRNYSSAREASLSNNHIPESVYDTLVD
                     VVNKHLPLLHRYMELRKRLLEVEKLHMYDLYTPVLGEAPITFTYEEAKEKALEALKPM
                     GEEYMAIVEKAFSERWIDVVENKGKRSGAYSSGSYDTNPYILLNWHDTLDQLFTLVHE
                     MGHSVHSYFTRSNQPYVYGDYSIFLAEIASTTNENILTEYLLETEKDPRVRAYVLNHY
                     LDGFKGTVFRQTQFAEFEHFMHTEDEKGVPLTSEYLSDSYGKLNAKYYGPAVEEDPEI
                     KFEWSRIPHFYYNYYVFQYSTGFSAASALAKKILNQEPEALENYLAYLKAGNSDYPVE
                     VMKKAGVDMTQAAYIEDAMSMFEQRLNELEELIDRL"
     gene            complement(16943..17941)
                     /locus_tag="IV334_001481"
     CDS             complement(16943..17941)
                     /locus_tag="IV334_001481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein CoiA"
                     /protein_id="HAP8943554.1"
                     /translation="MLLAMNEYNKCCFAKHAEKGKKYYCRGCRKRVILKKGKKKCAHF
                     AHQKSDNCSVFSERESKEHLQLKECFMDWLGQSVEPVFLEAYLPRLRQRPDILLANLA
                     IEIQCSRLSHQRFIERTQNYLNNSYQVWWILGNSFLGQSQFSLIEKSCCYYNRKRGVH
                     CWKADLKKQKLYLYHHITETVSGHISFCSSCWTFSSRDLKEIFTSNEIKMNQMKKIEK
                     VSEDGKKWLTRQLIHKQKNTVSIQEQCYLRHKHLLHLSSWIYQKSRFFFYLQEQVFLY
                     RMLYEEALENQKVPDFNSWLCQIKEHKREWLFPMIDEEMVYQQFFNECIHLSSLKK"
     gene            complement(18167..18820)
                     /locus_tag="IV334_001482"
     CDS             complement(18167..18820)
                     /locus_tag="IV334_001482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adaptor protein MecA"
                     /protein_id="HAP8943555.1"
                     /translation="MEMEHINENTIRVLIGHEDLEERGVSFLDLLGNHKEIENFFYSI
                     LEEVDIEEEFRGSEAVTFQVLPKGDGLELFISKNLPDEEISQSEDSTVDSEDVSDFLR
                     QQIIGSDPEEELSGSALEEQERQATFAFEDFEQVIQLATDVELESAWTDLYLFEERYY
                     LVVHFWMENLNQADVENQIARILEFAEKSDRTPETLNEYGQCLMARNAIERTKFYFV"
BASE COUNT         5525 a         4137 c         3323 g         6145 t
ORIGIN      
        1 acgtaaagct tgctgccaaa ttagcgattg aaactaacga ttgctttcta ggaatcaagg
       61 acaagcgaat accatttgac gattgatttg cttgctgatc ctgtacattg cggacccccc
      121 acatcgtccc gtagagaatc gccattccaa tcatagtgaa gaaataaaaa cttttgaaac
      181 tcatattacg acctgcatga acttcttgaa aagatacttc ttgttcaaaa acagtcatga
      241 tgggcgcgag atcaacgttc tccacttctg ccactttact cgaaattata ccaatacgct
      301 gtaaaaattg atccatgacg tttttcatca acgtctgact cattcctgct tgacccacat
      361 ggagcgaaac agatgaacca tcaatgagat aaaaaccagc caaatcatct gcatttagct
      421 tttcattcgc ttctacttcc gatagttcct gtacttcaaa taatggttta tcattgactt
      481 cgacttgttc taagactgtg gtaaaatttt ccgaaagcgc tgtttgatcc gctttgatta
      541 caattccagt attaatcgtt tcgagattgc caccttgatc aatctcgcca aaggccgcca
      601 taaacatcaa tcctaaaaga attggaaagg ccaatgtcca aaacaacatg gatttatttc
      661 gcagcagtat ttttgtgcga tatttataaa aatgccaaaa cattatttcc cctcctctag
      721 tctctcaaat ccttgccagt aatctctaaa aagacatcat tcaaggacgc ttctttggtt
      781 gctatttttc cgtaactgat cttttcttct tgtaagattg taagaatcgg caacaatact
      841 gtatttagtt cagtagttgt tacttggagc tgaccttttt tataagcgat tccatcagaa
      901 gctattgttc gctgacaaca cgttttctgg gagtaatggg atgtcgatcg tcaaacgatc
      961 cgccgtccgt accatatctt ttagagattc tttggttcct ttagcaatca cctgtccttg
     1021 atccatgatc acgatctggt cgcaaagaat ttctacttct tccatgtaat gtgttgtata
     1081 gacaatggtt gctccttgtc gattcagctc cttgatactt tctaaaattt tattccgact
     1141 ttgggggtct acagctactg tcggctcatc taaaaaaatt aaccgcggct tgtgggcaat
     1201 cccacatgcg atgttcaacc gacgtttcaa gccaccacta agttctcctg gacgaaacga
     1261 aacatatttt tctaatccca ctaattgaat cacctcttca atcaaggctt tccttttctc
     1321 tttttcggaa acatataaac cgcaaaaata atcgatattt tcttgcacac ttagttcgtc
     1381 aaagaccgca acgtcttgag gaacgacccc aatcattgcc ttgacagcat aattttcagg
     1441 agtcattgtt gtcccgaaca atttaatggt tcctttgtca taatttaaca aagaaagcat
     1501 acagttgatg gcagtcgatt tcccactgcc gtttggtcct aacagaccta agacttctcc
     1561 ttcttgtact tgcagatcaa aatgattcaa tgctacaaaa tctccatagc gtttgactaa
     1621 tttatcaatt tcgataatca tcatgaaaaa ctccttccaa atttcttgtt gatctgacta
     1681 taataaagtg caagaaaaga aactagtgac gattggctaa accacacatg acatttgtca
     1741 caatgaagga gtctgctatg cataaatctc ggctactcaa cgaaactagc tttctactgg
     1801 ttatctcatt gcttttgctg gtaagtcaag gattcgttgc tatgcaagtt atctatttat
     1861 tgatcgccgt tttattcact ggtgtcagtt atttactgcc tacaaagcca aattggattt
     1921 tccaagttat tctatttctt ttatgtgcct ttttacctga tcttgtcctg tttctgccag
     1981 cgacgatgcg agtgatcgcc cctgaagaaa gccaccttga ttacttgttt ttgttaggtt
     2041 ttttgttgat ttcgacggtt ggtaatcatc cccccatcaa ggtcgccttt gtcgtaattt
     2101 tgctgttttt ttccttttac ttacgaaaaa aagatcaacg aacgaccgat cttgagaaaa
     2161 agtacattgt ctccaaagat actcattggg aaaaacaaca agagttatcg gaacaaaata
     2221 aagcattggc cctgtcacaa gaaacacaat tagccttgca aattgtggaa gaacggaacc
     2281 gaattgcccg agacatccat gacaatgtcg gtcatctgct gtcaagtgcg attttacaaa
     2341 ttggtgcgct tgaaatgatc aatcaagaac caaaactaaa agaaccatta gaaaatttaa
     2401 gtgccaccgt tcacactggg atggatcgga ttcgtcaaag tgtccatgac ctccatgaga
     2461 catcgatctc ttttcaacac tccttagagt ttctgatcgc tgattttcct tcttctgtta
     2521 cgattgaagg aaacttgttt gaaggattga atgaaacgca gcaaaactgc ttcttaatgg
     2581 tcatcaaaga agccttaacc aacagtatca aacatagtca tgccaaaacc gttgttttac
     2641 attttcgaac gttgccggcc ttttatcgct tgcaaatcac gaatgatggc accagcccac
     2701 aaaaaaattc tgctcccggt attggtttga aaacgatgcg tcaacgtgtt caagaacaaa
     2761 agggacaagt ccatattttc caagaaaacc agcaatttca gttaacgatc attttaccaa
     2821 gggagggaca ccaatgataa cagtagtcat tattgagatg atccatttgt gacgaaatcg
     2881 ttacaaacca ttttagaaag cacgaaagag atccgtgtgt taggcattgg tcattgtgcc
     2941 aaggatgcgc tagtcttata cgaaacccat cgtcccgatg ttttgctgac ggacatccgc
     3001 atgcctaaac aaacgggcat cgatgctgcc aaagaaattt tggctaaatt tcccaaagcc
     3061 atccttttgc ttttgacgac attcaaagat gaagaataca tccgcgaagc attttccatc
     3121 ggtgtcaaag ggtatttaat caaacagaac ttgcaagcga ttattccttc tgtcaaatcc
     3181 gcttataatg gacaggccgt atttggtaat gaaatcatcg agactttcac tcaaatgatg
     3241 aagaataaac cgacgattga tagtcatgct tatgcttctt ttagtgaacg tgagcttgca
     3301 atcatcaaag aagtagctgc cgggaaaaac aataaagaaa ttgcagatgc cttgtacttg
     3361 agtgacggca ctgttcgcaa ttacatcagc caattactag aaaagctaga tttacgggat
     3421 cgcacgcaat tggctatcta tttttatcag catttgcaat aagtcttatc taacacaaaa
     3481 aaacctgaat gatgctcctt cgcatcattc aggttttttt cacatcttct attcaattgt
     3541 cccgcaaacc atttcctcac gattgaaaga atcaaggatt cgcagggctg ccaggattcg
     3601 ttggattgtt cggcgtgtta ggcggaaaaa taggcgcctt gtctaacgtt ttttctagtt
     3661 ttgattcttc acgatcgtct cgttctacat caggactttc attcaatttg aattcttttt
     3721 ccatctcatc taacttttta aagtcattgc cagattcttt tgctaatttc gctaatttat
     3781 cttcggtttc tttagccata aagtaacgcc ccccttttga tttcagctta gcatatcaca
     3841 ctgaaaaaac aaaagaaaac gctaaccaac gatcattttt agctatttct catctttaaa
     3901 atcaagcatt ttttgccggt ataaaatcgt agggagaact gttttccata ccaatcatcg
     3961 gatctactaa ctggttatcg atcacttctg ctgtaagaac gaaatcttta gaaacagcag
     4021 gttctacctc ttctttcttt tcttctccaa caaatgaaga aaacagatct ggttccactg
     4081 tttccattac ttggtttttc gtttcattcg tcccactgtc atcttctaca ggtagctttt
     4141 cttttacaac ccgtggctct tttgtatagt tcccatcttc aaatggatca tttccgacca
     4201 aagaatcttc ataagctgct agtttggtcc ggagagtgat attcatttta tcattatcca
     4261 tgatccttga tttcaacgta gtcatccggc ttgctgattc attcgctaca caagtgacat
     4321 aggcttgctc ttcccctaac aaaatcaaca gttccttact gcgagtcact gccgtgtaga
     4381 gcaaattacg ctgaagcatc cgctgatatt gatggaccat gggcaaaata accatcttga
     4441 attcactgcc ttgtgattta tggattgagc aacaataaga aagagtgatt ttattccatt
     4501 cgtttctttt ataagtgacc tcatttgcat caaactggat aactaattca tccactttat
     4561 cttcagaatc ttttgccaag atgatacccg tgatttctcc catatctcca ttgaatacat
     4621 tcaattcggg agtattgact aattggagga ctttgtctcc tatccgataa acggtgtcgt
     4681 tccaatgtac ttcctttttt ccaggtttcc ctggattgaa tatctcctgc atcattttat
     4741 ttaacgcatc gatccctgca ggtccccgat acattggtgc taatacttgg atatcttgtg
     4801 cacggaatcc cttatttttc gctttttcca caattttaga gatcaagggt tcaatttgat
     4861 aagcatcact tcggaaatag gaacgatctt tttgattctt tgtgaaatct tttggcagac
     4921 gtccttcttt gatctcatgg gcaagaggaa taatactaga accatcacct tgccgataaa
     4981 tctgattgag ttccattttg ggaatttgtt cgatttccaa aagatcatgg agtacttgac
     5041 caggaccgac agagggaagc tgatctttgt ctcctactaa gataacttgc atattcgtcg
     5101 gaatcgcttt gaataatgtg ttagccagcc atgtatcgac catagacatc tcatcaacaa
     5161 tcaaaagtcc gccttctaat tctttcgtac tgactgaagg tgttttttcc cgtccattca
     5221 gccccagaag acgatggatc gtactggcag gcagacctgt cgtttcgttc attcgtttag
     5281 ctgctcggcc agttggtgct gcaagtagaa taggaaaaat ttcttgggta tattttgaag
     5341 gatccaattc cagtccgttc aactctgcaa aaagattgac gattccgttg atcacagtag
     5401 ttttccccgt acctggtcct cctgttaaaa taaatagagg ggaacggatt gcttcagcga
     5461 tagcttgttc ttgcgaaggt ccatactgta tgccgaatat tttttcgata cgccgaatat
     5521 tcttctgcac ttctttttca ggataatgga tttctttttt acgactgaga aggcgctgga
     5581 tcgaattacc gatcccccat tcagaaaagt acaaactgtt ttcgtaaaga ttagtctctt
     5641 cttgttgtat ctttccttct tcaacaagtt ggataattac agtagcaacc tgatcaggct
     5701 ggatttcaat tgggcgactt gtttctaaaa tacggatcgt ttgctgaagc aatgtctccg
     5761 ccggtacgta tgtatctcct gtttgaactg catgctgaaa aatctgatgc aagatagctg
     5821 ctcgaattct tctcggtgaa tctgctgcaa taccgatttg ttcggctaga ttatctgcac
     5881 gtttgaaccc gattccttct atgtcttcga ctagctggta cggattttct tcgatgatat
     5941 ccaaagactc gttcttatag gcctggtaga tactgaaagc taactgacta ccgaacccgt
     6001 aacgattcag tcccatgatc acttgatcca tcccgtggtt tagccgaatc gtctcagcaa
     6061 gcatttctct ttttggcttc gtcaaaccgg agatttgttt taataccgaa ggatcagcaa
     6121 tgattttttc gatagcatct tctcccagca attctacaat tttttctgct gttttcttcc
     6181 cgattcctgg gaatttttcg ctggaaagat acgctacgat cccatttgca gttgttggct
     6241 tttcttgctc atatctttcg agtttaagct gccttccgta tttaggatga tccactaatt
     6301 ctccataaaa acgataagtc tcattttctt gaatatcgcc gaaacttccg gtgactacaa
     6361 tctctttttc acggtaatca gctgtggttt cactgatgtg taccaaaagc actttgtaaa
     6421 aattactagg attttgaaaa aaaatcgcgg caactgtacc aacgaaataa ggcatttctt
     6481 ctggctgcat cggaagccgc ctccttcttc tatagtaaag gttctggtgc tgtttcagtt
     6541 cctaaaaaat ctgtttggtt ccatactttc agttcatatg gcaataatct gcaaggctct
     6601 tcagtctcta aaatcgtcaa actactattg aacaatccac ccatttcacg caactcactt
     6661 agctcttttc ctgtcatcca ttgaatggct gcagttaaag aagctccatg tcctacgaat
     6721 aaaagtgggc catctttatg ttgggcaacc gcatctgcta caacagtttc gatacgtgtg
     6781 attgccgatt caatcgtttc tccgtgaaaa ggagaaggat catacagatc taaatgatta
     6841 cgcaaattcg gcagattatc cggatatttc tggtacattt catcgatcga ctgaccttct
     6901 aaagtaccca gccctagttc tcttagctta tctgtataaa aaatctctac cggacgagca
     6961 agctctttgt tgataccttc tgctgtatca cgtgctcgct ttgctgtact ggaataaatt
     7021 gctttaaaag gaacatattt tacctttttc ccaaatgctt tgatttcttc gtaactttgt
     7081 ggcaaaagcg gagaatcgcc attgctccct tgaaaacgtc tttcctgatt ccattgtgtc
     7141 ttcccatgtc gggtaaaata tagttccata cgcagcaaac tcctgtcttt cttttatttt
     7201 actctataat aaactgtcta atccagcttg tcattttcat agtgatgcgt caattctaaa
     7261 ttagggaatc cttgctggcg aagtgcttca tacactatca ttgcagctgt attcgataaa
     7321 ttcaatgagc ggacatgctc atcattcata gggatgcgta aacacttttc ttcgttttca
     7381 cgcataaatg tttctggcaa accggttgtt tcttttccaa ataaaaagaa gtgctcttct
     7441 ccatcagcaa aattttcgtc actatatact ttatgggcaa attttgagat caaatgtagt
     7501 ttacgattac ctaaaaatgt taaaaaagct tctaaattac tgtggtaagt aatatttaca
     7561 tcattccaat aatccaaccc tgcccgtttt agatgtttgt catcagttga aaaacctagt
     7621 ggctcgatta aatgaagcgg agtattcgtg gctgcacacg tacgggcaat atttccagtg
     7681 tttgcaggga ttaatggttc aaataaaacg atatgattca tcaggatcat tcctctcata
     7741 tttatgttcg tttctatttt ctgacaattt gatttttgca taaaaaaaaa cgtatcaact
     7801 cggtgtcagt cactgcgagt cgttacgtta gtgaagctct tctcactaac ttttatcaga
     7861 acaggaacct gataaaaacc aatgaaaaaa caacttgttg ctaatataac accctcaact
     7921 tttttttgca acctctactc attaaaaact gctagaaaaa cgttttctct tagttccttt
     7981 taataattct atgtcaattg taatcttctt caaaggattc ttatataagt ctgctttgat
     8041 tcgctcttct acctgccatt ctaaaggtga catggccaaa agatcttttt gattttcctc
     8101 tggtatctca aattcatagg ttacacgctg acgttcagtt aatttcattg cttcagcaaa
     8161 cttggctact acacgttcat tggaataact ctgtttttca ggttgattcg gataaaaagc
     8221 ctgacgtagt tcctttaaat aattttcttg tggcaccacc ttcaacactt ctccatcttc
     8281 tgagagtaca cgttggaatt cttggtaatg agaaggcgaa aagatattta aaaccgcgtt
     8341 gaatgtttga tcagcaaacg gcaaattggt caaatcagct acacaccaaa atgcttcggc
     8401 agattgttct gtagctgcgt agactccttc ttttgaaata tcaaatccaa cagcattttg
     8461 aatctttttc aattttgaaa gttcattcaa gaagctacct tctccgcagc caacatctaa
     8521 tagcgagact tcttcaggta aatgctgcgc aatagtctca ataacgggct gatacatgcc
     8581 agattggatc attctacgcc ttggaacgaa cattccttga tcgtattctg ttttaaccgc
     8641 atgggataaa aaataaagtg ttcctttttt ggaaaggtca aaacggtgat tatttttgca
     8701 tatcaagcca ttatcagact tttttacttc ttcttggcaa accggacagc ggaacagctg
     8761 atgattttct gccaaaaact ggcgtcctcg gtcaattttt tttaacatag ttccccctca
     8821 tttctgtttt atcttaccgt tagatgctca ctttggcaac tgctaaaaat atgatacaat
     8881 gcctagaaga aagtgaaaga taaaggagaa gattagatga tcaaagaatt ttgcgctgaa
     8941 aactacacat ctattcctct agcaattgct agaggagccg atcggatcga attgtgtgac
     9001 aacttagcgg tcggtggcac aaccccgagt actggtgtga tcgaagaaac gttaagttat
     9061 gctggggaaa aagatatccc cgtaatgacg attattcgtc ctagaggggg cgacttcgtc
     9121 tataacgata tcgaattgaa aatcatgcat actgatttga ttgaagcaaa gaaattaggg
     9181 acggatggcg tagtatttgg ttgtcttact ccttcaggct ggctagacga agaggcctta
     9241 gaactattga tcgaaacagc agaagggttg cagatcacct tccatatggc ttttgacagt
     9301 attcctgaag atcggcaatt cgaagcaatc gattggctgg tagaacatgg tgttgaccgt
     9361 atcttgactc atggcggtcc tgctgacaaa actattgtag aaaatttccc acggcttaaa
     9421 gaattgattg cctatacaga tggccgctta atcattttgc ctggtggtgg tgtcactgca
     9481 gagaatgcag cacatgtagc taaagaattg aacgtaagcg aagtacacgg aaccaaaatc
     9541 gtggatcttc agttataatc ttataaacaa aaaaacattt gcaacaggca taatgcttat
     9601 tgcaaatgtt tttgtttttt tacataaaat agcccataaa taaagaagca atattgaaat
     9661 aaatcagtac actcgtcaaa tcgctcaaag tcgtgataaa gggaccgctt gctactgctg
     9721 gatcaaatcc tagacgatcc attagcatcg ggattaaact acctgcaaga ttcgccactg
     9781 tgattgcaaa tagcatagcc atccctatga caaaccccaa gataaaatta tgtttccaaa
     9841 aacctacgat tagaaaaatc gtgatccccg taacagctcc tgtgacaacg ccggtcaaga
     9901 cttcagccaa aacaagtcga acaaatccat gatctttctc atcagaaatc gcaagtcgac
     9961 gaacagccac agctagcgac tgggttccag cattaccggc agttcctgta atcagcgaaa
    10021 tgaagactgc taaaatactc gcttcactta ccaagtcttc atagtgactg attaaagaag
    10081 cggtcgacat acccaaaaac aacagagtga tcagccaagg caaacgtcga gacgctgcct
    10141 ttactggatt ttcattaatt tcttccacat tgaccccggc aagtcctgaa taatcgctag
    10201 cagcttcatc atcaataacg tcgatgatat catcgactgt gacgattcct aacaaatgat
    10261 catcataatc ggtaacagga agagctaaga agtcataatc tctaaatgtt tgtgccacat
    10321 cttcttggtc gtcgcctaca tgaactgaaa gcactcgttc gctcatcaga tcagaaatca
    10381 ttgcgtcgtc atcattcacg attaggtcgc gcagagaaat aacccctact agctgacctt
    10441 cttgattaac gacatagatg taatagattg tttcagctac atctgcttcg ttcttcagca
    10501 cgtacatcgc agagcgaaca gtttgattgg ctacgatcga aacaaattca gttgtcatga
    10561 tcgcgccagc cgtttcatcc tcgtaatgga gaagttcctt gatttcactg gcatcgtctg
    10621 ccgacatcaa acttaaatat ttagcaattt gctttttatc cagcgtatta agcaagtcaa
    10681 cagcattatc tgtatacatc tcagaaagca tatcagctgc atagctagga cgcatttcat
    10741 ctaggtattc tttcatatgt tcgttgtctt cttcaatcac atcgaacata tccgctaatt
    10801 ctttaggcga cagataggaa tagatcagct gcctgttctc ttctgtaaga gattggtaga
    10861 attgcccctg ctcatagata tgcatctcta aaaaactgtt tctgaatgcc tgaatattat
    10921 tttcccttaa atcttgagtc aactgggcaa atctttcctc taattcttga ttttcatcca
    10981 aaccaatctc acctcgcctt attcttcaaa ccgtctcaca actgcctcca tgtctgcggc
    11041 taaaggctgc ttgaactcca gcaactcttc tgtaaatgga tggtaaaaac gcagatcata
    11101 acaatgaagg gcttgtcttt ttattcccag atccattcgc ccgccataca tatcatcacc
    11161 gagcagggca cagccaattg cggaaaaatg aactcttatt tgatgtgtcc ttcctgtatg
    11221 cagctggata tcaactaacg cgatttctgt tagtcgcttc tctatccagt actcagtctg
    11281 tgccgaacgt ccttcatcag agaccattcg cttcaatagc gaagaataat ctcgggcgat
    11341 cggcaaatcg atatgtccat gtgccgataa atctgaaagc cttccactga ctaaggcctg
    11401 atattttttc acaaacttct tttcacgaag ttgcatatct aattttgcgt gcgcaaatcc
    11461 atgctttgca aaaagcatca aaccagatgt atcccgatct agtcgcgtca ccacatggat
    11521 tacttggttt tcgtaacctt gttttttata ataagccttt acacgattag ccattgtccc
    11581 atttggatgg tattgggcag gaatagacga aacgcctgct ggtttattta ctaccagaac
    11641 gtgttcatct tcatacacga tatcgatcgg tgtttcatcc aaaagtacgg tttcatgttc
    11701 tccttcagca ggaatcacaa tggttacctt atcatcctta gctagagaaa acaagacatt
    11761 ttctacttga tcattaactt tgatttgacc gccttgaaat tttattttcg ctaacagacc
    11821 ctttgagatc cctttttctt ttaaaaaata ttttacttgc tgagaatgat ctttctcata
    11881 atcccatgta aactgcatat caatcctcac taataaaggc atctttcaca cgatgccaaa
    11941 aatgcatatg tcgataagaa gcaaaatgga tccgttcttc tgctattcga taataaattg
    12001 agcggatatt tgcttttgcg acattcaatt gatcaaccgt tacaagataa tcttctgtat
    12061 tttccaattt tatttctagc cattcatcat gggcaatcac gatcggagaa cccaaagtac
    12121 gaaacactcg attattcagt gaagcaatct ctgccaactg aaaggcattg atactaggat
    12181 ggatcacagc accgccgaca cttttattat atgctgtcga accagtaggc gtagaaatcg
    12241 tcaatccatc tcctctaaaa ctttcaaaca gctcgttttt gatgtataca tcggctacca
    12301 tcgtacgatt ggcccgttta atcgttgatt cattcagtgc taaaaaatgc tggtccggtg
    12361 tttcatccaa ataactgatc cgaacatcca gcaagggata actaacactt tgttcccggt
    12421 tcgttcgtaa gctgtctact agttcttcca gctcgtaatc ccgccaatcc gtgtaaaacc
    12481 ctaaatgacc tgtatgcacg cccaagaaac gcacatcatt cagacaatga ctgtagtgat
    12541 ggaacgccga caataatgtg ccgtctccgc cgactgagat gaccaattct ggctcatgat
    12601 catctcgttg aatccctgct tgttctaaaa gtgcgttcaa acgttctgtc acttctatag
    12661 tctttgcttc tttattatga acgatcgcta ctttcatccc ctgcctcctc tctttcgctt
    12721 tctttcttca aaagcgttcg atagtagaca tcatctgatt ttcctcttcc atgggagaat
    12781 aaccgctgtg cttcttggat ttcttcacgg atactggaca tctcttcatc caactgatag
    12841 gcagcttctg ccgcacgctg aagtcgttca ctcatttctt ctggaaaaac gccttgatat
    12901 ttataattca gcgaatgctc aatcgttgcc cagaaattca tcgcaagagt ccgaatctgg
    12961 atttccgcca aaattttctt ctcaccgctg atcagctgta caggatactc gatcaccaga
    13021 tgataagaac ggtagccact tgctttttta tttgtgatgt aatctcgttc ttggagaact
    13081 ttcatgtctt ttcttttacg gatgatctca acaacttgat ggatatcttc tacaaactga
    13141 cacatgatcc ttaacccggc aatatcagac atttcttctt ctaaacgtcc cattggaata
    13201 tgccgtaatt gtgattttgt tacaatacta tcgactggct tgactctccc agtgacaaat
    13261 tcaatcggga catgtcgatt ttgttcacgg aactgtttgc ggatcccccg cagtttcact
    13321 ttcaattctg atacagtttg ttcatatggt gctaaaaatg cttcccaatc tcgttccata
    13381 gctgatacct ctacttcatt aacattcatt gtttattcta ccatatatgc aaagtttttt
    13441 cgtactcttg atttatgcat tgaccaaaaa aagaaaatta ggcaaaatag acaaaaagga
    13501 gtcgatcaaa tgacacaaat gcttgaaatc gaatataaat ctatgctgac aaagcaagag
    13561 tacgagatat tcatcgctca ctatcaatta acagataaag atttccgtgt acagactaat
    13621 atttatttcg ataccgttga tgaacagctg aagaaacaaa attgtggcct acggatccgt
    13681 ttcgttggtc atcaagctga atatacatta aaaacacctg ctgaaaaagg gcgtctcgaa
    13741 acaacggata cctttataac agaagaagca gaagcattta tcttcgaaaa acgtctgcct
    13801 cgttcaggcg cagtttttca aaaactttct gatttgaata tcgatcctgc ttcattaatc
    13861 caaaccggaa agcttacaac aaaacgggct gaattcccta ttgaagaagg acttttagct
    13921 attgatgaaa gctggggaga caatctacac gattttgaac tggaactaga ggtaggggat
    13981 gcatctgtgg gaaaaattgc ttttataaat tttttaaaac gtttttcttt accttatcgt
    14041 cctgctaaga acaagatcca acgtatgctt gaagctaaaa attagaaacc tttcattttc
    14101 acaaggcact tgagttcatt tttttacttt tttctgttaa gttataggat gtgaaagaat
    14161 ctagtcacta gttgtataga atctattttg ggggagattc atatgatcga aatctattta
    14221 tttgttaatc ctttagggga atactgtttc gaaatggaac aacagttatt acaatttatc
    14281 gaagaagagt acgggacacc atcaaaagaa aagatgcaat ttcgctttct tcctttggtc
    14341 aatttgcaga caattgggga tgtcatgcaa caaaaaggaa tttctcagaa caatcttgag
    14401 gaacgtaatc acttattctc aatgacttat tctgcagcat tggactgcaa agcggcgcag
    14461 tttcaaggaa agaaaaaagg ccgccgtttt ttgatcaaat tgcaagaagc agtcggctgt
    14521 aatgacatac catattctca agaacttgct gaatccatat ttgaagaaat cggcggcgat
    14581 ttgaatatgt ttaaagaaga tcgtcagtct gacttcgtta aagaagtatt cttttctgat
    14641 caaaaaattg ctcgggaaat gggcatcaca aaacaccctt ctgcagtcgt ttacaattat
    14701 gcctgcgacc gtgattttgg tgttttagta gaagtaaaag gtacaagtag cctttggaga
    14761 atcacagagc tgtgtaaaac aacagataac tcatatcaag tttttaatgg tgaacattta
    14821 catacaaatg attctcagtt ccattcaaga actagccatt tacacttgct ttctaattaa
    14881 aaaaatacgt cgactccctt acaagagagc cgacgtattt ttttcgtttc tattcgagaa
    14941 aatcaaaaat agaaagcctt ataaacgatc gatcaattct tctaattcat ttagacgttg
    15001 ttcaaacatc gacattgcat cttcaatata tgcagcctgt gtcatatcaa ctcctgcttt
    15061 tttcattact tccacaggat aatcgctgtt acctgctttt aggtaagcca agtagttctc
    15121 tagtgcctct ggttcctggt ttagtatttt cttcgccagt gctgaagcag cagaaaagcc
    15181 agtcgagtat tggaaaacat agtaattata ataaaaatgc ggtatccgtg accattcgaa
    15241 tttaatttct gggtcttctt cgactgctgg accataatat tttgcattca atttaccata
    15301 actatcactt aggtattcac tagtcaatgg cacacctttt tcatcttccg tatgcatgaa
    15361 atgctcgaat tcagcgaatt gtgtctggcg gaaaactgtt cctttaaacc catccaagta
    15421 atggttgagt acataagccc gtacacgagg gtctttttct gtttccaata aatactccgt
    15481 taggatattt tcattcgttg tcgaagcaat ttctgctaaa aagatggagt agtcgccgta
    15541 cacataaggc tggttcgaac gagtgaaata actatgaaca ctatgtccca tttcgtggac
    15601 aagcgtaaat agctgatcca gcgtatcatg ccaattcaat aaaatatatg gatttgtgtc
    15661 atagcttccc gaagaataag caccgcttcg tttcccttta ttttcgacaa catcgatcca
    15721 acgttcagag aatgcttttt ctacgatggc catgtattct tcacccattg gtttcagtgc
    15781 ttctaaagct ttttcttttg cttcttcgta cgtaaaggta attggagctt cccctaagac
    15841 cggtgtataa agatcataca tgtgcagttt ttccacttct aataaacgtt tccgtaattc
    15901 catgtatcga tgtaacaaag gcaaatgctt gtttaccacg tctaccaaag tatcgtatac
    15961 actttcagga atatgattat tgctcaaaga cgcttctctg gcagagctgt aattacggac
    16021 tttcgcttta aaattatgtc cttttatatg tgtgcctaaa gtagaagcaa atgtatttct
    16081 aaattgttcg taaacactgt acaatccctt aaatgctgct tcgcgcaccc ttcgatccgt
    16141 gctttctagc aactgaccat acacgccatg agataattgg actatttcac cattttctcc
    16201 ttcaatcgtt ggaaaaacta gatctgcatt attcaaaaca gcaaatgtat cacttgaagc
    16261 atcaaagatt tcacctgctc cagcaagaag agattcctgc tcagcagata agacatgggc
    16321 tcgattatct actatttgct ggatataatg gcgatagact tccaattttg gttcttcttt
    16381 gaaatattgc caaatctggt catctgacaa ttgcaatatt tctggttcaa accaggaaac
    16441 ggcttcacta actttcgaaa acagactgct tgctcttgca taaagcgctt ggtaatctgt
    16501 atttccagtg tcttgatcgt ttttaaggtg cgcatataca taaatgactt cagtttggcg
    16561 ataaaccctc aatacgaatt caatcgcatt taaaaattga gaagcgcctt gatcaagtgt
    16621 tcctttgtgt ttttcagatt gttttaactc ttctgataat tccaaatatt tctcatcaaa
    16681 ctcttggtcg cttgaaaaga tcttggtcaa gtcccaagtt aaattttcag gcaattcttc
    16741 tcgttttggc aactgcttta cttccatgaa atccactcct tttcttccct tagctctatc
    16801 tatcaaaata gatcatagtc taacatccac atgtcacagc atctaacttt tctactcatt
    16861 catgtgtctg cttccatcaa ttttagcata actaaatctt gttttacata gaaaaccgat
    16921 tcctagccta tttttatact tactattttt tcaaagaaga caagtgtatg cattcgttaa
    16981 aaaactgctg atagaccatc tcttcatcaa tcataggaaa aagccattcc cttttatgtt
    17041 cttttatttg gcaaagccat gagttgaaat caggaacttt ctggttttcc aaggcttctt
    17101 cgtacaacat tcgataaaga aaaacctgtt cttgtaaata aaagaaaaac cgactttttt
    17161 gatagatcca cgatgataga tgcaaaagat gtttatgacg caagtagcat tgttcttgta
    17221 tggatactgt attcttttgc ttgtgaataa gttggcgtgt cagccatttc ttcccatctt
    17281 cactcacttt ttctattttt ttcatttggt tcattttaat ttcgttacta gtgaatattt
    17341 ctttaaggtc cctactagaa aaagtccagc agctactgca aaaggaaata tgccctgata
    17401 cagtctctgt aatatgatga tacaaataaa gcttttgttt cttgagatca gctttccagc
    17461 aatgcactcc tctttttcta ttatagtagc agcaactttt ttctatcaat gagaattgcg
    17521 attgtccaag aaaagaatta ccaagtatcc accatacttg gtaactgtta ttcaaataat
    17581 tttgggttct ttcaataaat ctctggtgag acagtctaga acattggatt tctattgcta
    17641 gattcgccaa caaaatatcc ggcctttgcc gcaacctcgg caaataggct tccaaaaata
    17701 ctggttctac tgactgtccc agccaatcca tgaagcattc ctttaactgt aagtgctctt
    17761 ttgattctct ttcactaaag accgaacagt tatctgattt ttgatgggca aaatgagcac
    17821 attttttctt tccctttttc aaaatcactc tttttcgaca gccacgacaa taatatttct
    17881 ttcctttttc tgcatgttta gcaaaacagc acttattata ttcattcatt gcaagcagca
    17941 tttttctccc tctgaactta tttacgtcta agaatttatt tacacgaaaa aagagggcgt
    18001 gaaaaagcta ttctgcatca agtaataaga acagctaaac aaaaatagtt cccaatattt
    18061 ttcgtttggt tgggcttgtt accgcagatg caagcttttg aacacattta tacgaaaaaa
    18121 agactgcgac acaatttttg tcacagtctc ttactataat gctgatttat acaaaataaa
    18181 atttcgttcg ctcgatagca ttacgcgcca tcagacattg tccatattca ttcaatgttt
    18241 ctggtgtacg atcgctcttt tcagcaaact ccaaaattcg agcaatctga ttctctacat
    18301 ccgcttggtt gagattttcc atccagaagt gaacgactag gtaatagcgc tcttcgaata
    18361 aatacaaatc agtccaagca gactctagtt cgacatctgt tgcaagttgg atcacttgtt
    18421 caaaatcttc aaaagcaaaa gttgcttgtc tttcctgttc ttctagggcg cttccagata
    18481 attcttcctc cggatcgctg ccaataatct gctgacgaag aaagtcactc acatcttctg
    18541 aatcaacagt agaatcttct gattgagaaa tttcttcatc cggaagattt ttactgataa
    18601 acagttctaa tccgtctcct tttggcaaaa cttggaaagt gacagcttcg cttcctcgga
    18661 actcctcttc gatatcaact tcttccaaaa tactataaaa gaaattttcg atttctttgt
    18721 gattgccaag taaatccaaa aatgaaacgc cccgttcctc taaatcttca tgaccaatta
    18781 gtacacggat cgtattttca ttaatatgtt ccatttccat tttgctacac ctcactttgc
    18841 cacaatgttt aatatagatt cattgtagcg aaatcagctt aaatgtaaag aaaaaaaatc
    18901 tcgctgttaa gcgaatttaa taaaaccagc ggatttttca acctttcaag ctatcttatt
    18961 taactataac atttttggca gacgaatact atatagccga cttttcagct aattttccct
    19021 ctctattttt cgtgcaatca tcatcttcta ctaccttttt atacatctaa tcaagaatac
    19081 tttttgtaaa aacaaaaaaa cttggaaaaa ctatctttgt aatagtcttt
//
LOCUS       DACDKM010000045        19122 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20834_2016
            SAMN12640370-rid9891663.denovo.045, whole genome shotgun sequence.
ACCESSION   DACDKM010000045 DACDKM010000000
VERSION     DACDKM010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640370
            Sequence Read Archive: SRR10040891
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19122)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19122)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742835.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742835.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:04:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,012
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 29 of 75
            Pseudo Genes (incomplete)         :: 33 of 75
            Pseudo Genes (internal stop)      :: 19 of 75
            Pseudo Genes (multiple problems)  :: 5 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19122
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640370-rid9891663.denovo.045"
                     /isolate="E20834_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..562
                     /locus_tag="IV334_001483"
     CDS             <1..562
                     /locus_tag="IV334_001483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000181735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HAP8943556.1"
                     /translation="LKNEFGIEVKDYFIDLDLLQLINSVKRMYSGDDNGVDLYKFVTK
                     HIFVNLNIDEKNYPSIFKHYLFFINEVEDRIKNNSLSSSVSSFKKMFKSRDGITLTTA
                     HKVKGNEYDTVIAINLLNGKIPHWNEIFNVSDKGISSARKLLYVICSRAKNNLFLFSE
                     VGYFTSKGDLLYPTQLLDKIEFDYNI"
     gene            762..1076
                     /locus_tag="IV334_001484"
     CDS             762..1076
                     /locus_tag="IV334_001484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018705633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HAP8943557.1"
                     /translation="MELKYVIPNMEKTFGQLEYAGEGNIEQRRINGRPTILSRSYNLY
                     STIQRADDIVVILPSEAGEKHFESDEKVKLINPHITAEGYKIGNRGFTNYIMHADDMV
                     KA"
     gene            1097..1474
                     /locus_tag="IV334_001485"
     CDS             1097..1474
                     /locus_tag="IV334_001485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001234189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HAP8943558.1"
                     /translation="MRLAEGIVIEKDQTFGTLKFSALRREVRIQNEDGTVSEEIKERT
                     YDLKSKGQGRMIQVSIPASVPLKEFDYNAEVELINPVADTVATATFRGADVDWYVKAD
                     DLVLKGQTKPHQKYKKEASESEK"
     gene            1484..2257
                     /locus_tag="IV334_001486"
     CDS             1484..2257
                     /locus_tag="IV334_001486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002388713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8943559.1"
                     /translation="MTNTPLILKEIPKDEAISFIRQYHYSKILPRLCKYFLGIFSEEK
                     LLGVVELGWGTQPLQTIRKLFPDSSLQTTDYLEIGKMCFLPEMNQTNYFGSQALSALI
                     KWLKEHTDCHFLYTLADGIEGKCGYVYQASNFFYCGYFKTSVYRDKQSWEKIHPRSAR
                     LLLEENARFEQVEKKHWLSQAFCEYKGIEKINGRMFRYLYPLTKEAKKLLGHTLYRRH
                     YYPKEKNLRFEKRIAYQKYEAISQPTFDKQARIYNTQLF"
     gene            2279..3682
                     /locus_tag="IV334_001487"
     CDS             2279..3682
                     /locus_tag="IV334_001487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001130245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF87 domain-containing protein"
                     /protein_id="HAP8943560.1"
                     /translation="MPLLHDRGERIKANDKHLVYHFVLISLLVVFLVVLLPFHWRALQ
                     SVNWQAVQLDTFFYQLHTPYFWSSILTALFVCGLISWLYYHYRIDSIKQLEHRQKLAR
                     MILENHWYEAEETQSEGFFKDLPSKKTKQRIRHFPKIYYRMKDGLIHIHVEITLGKYQ
                     DQLLHLENKLETGLYCELVEKTLKDSYVEYVLLYDTIGNRIGIEDVVAQDGRVKLMES
                     VYWEFDSLPHMLIAGGTGSGKSYFILTLIESLLHTNAKLYILDPKNADLADLGAVMNN
                     VYYRKEDMLACINQFYEDMIDRSETMKQHPNYKTGENYAYLGLSANFLIFDEYVAMMD
                     MLGRESTTVIHKLKQIVMLGRQAGFFLILACQRPDAKYFSDGIRDNFNFRVALGRMSE
                     MGFGMMFGSDTQKQFFLKPIKGRGYVDTGKSVISEFYTPLVPKRYDFLGEIGKVIQKK
                     QSEPVPHEVKGTGSDLL"
     gene            3864..5048
                     /locus_tag="IV334_001488"
     CDS             3864..5048
                     /locus_tag="IV334_001488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000398284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HAP8943561.1"
                     /translation="MENEKIWIKELKEKRLAYGVSQNKLAVASHITRPYLSDIETGKA
                     VPTQQVKEDLLNALERFNPDNPLEMLFDYVRIRFPTNDVAQIIGEVLRLNMDYMLHED
                     FGYYSYPEHYRFGDIVVLVSHDVSKGVLLELKGKGCRQFENFLLAQHRSWYDFFQDCM
                     EHKGIFKRLDLAINDKTGILNIPDLAKKCKQEECISVFRSFKNYRSGELVHRDEKPYM
                     GNTLYIGSLKSEVYFCIYEKDYEQYVKNDVPLEDAEVKNRFEIRLKNDRATQAMKDLL
                     AHQQAEKTAFEIINRYIRFADKDDTKRRSDWKTNERWEWFIGKNRGALRLTTQPEPYS
                     FERTLNWLHHQVAPTLKIASILDVLNGTTIISTMIQEAKLTEKHEKLIEQQHLAMEDL
                     IT"
     gene            5045..5335
                     /locus_tag="IV334_001489"
     CDS             5045..5335
                     /locus_tag="IV334_001489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000055367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8943562.1"
                     /translation="MSLLTKELKKLGFQCGIEFQAYIQNTGKYTSLIIEGKRQAGDTI
                     YTYDFYKVRFYNNYTNRVTVYGEHLTPFQLLRRVKSYIYYREKYLKERRTIT"
     gene            5332..5553
                     /locus_tag="IV334_001490"
     CDS             5332..5553
                     /locus_tag="IV334_001490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002570387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8943563.1"
                     /translation="MNFGQNLYNWFLDNAQSLVLLAIVVIGLFLGFKREFSKLIGFLI
                     IALIAVGLVFNAAGVKDVLLNLFNRIIGA"
     gene            5595..6374
                     /locus_tag="IV334_001491"
     CDS             5595..6374
                     /locus_tag="IV334_001491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000678782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="abortive infection family protein"
                     /protein_id="HAP8943564.1"
                     /translation="MKGFMNMFTNDQFKSLLIKTLERKSSSNYYEGGNEIVSKMKISE
                     VTLDETDDFTYYKGYKKGWNTLCTYLKIRVPFDDLDFFESHKDLITQTASSIYDKQGD
                     NVLVDTILVPLPENYEVINFSQLKISDVVSQAIEDAESFMSNGEYQRAFDRVHTAFHG
                     YLIEILKKYEITVPRDENLSKLYSRIQQLIEKEIQPTELADIVKTTIRSSNGMISSLN
                     EARNRHSLAHPNTNIIGKREAKLIIGISSTVTDYISGYLDK"
     gene            6441..6935
                     /locus_tag="IV334_001492"
     CDS             6441..6935
                     /locus_tag="IV334_001492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000870467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HAP8943565.1"
                     /translation="MKVYVANLGRYNEGELVGAWFTPPIDEEEMAERIGLNEDYEEYA
                     IHDFELPFDVDEYTPISEINRLCEAIQEIEGTPIYNELKEIQGMWFSSLEELLENKED
                     IHCYSDCDSMEDVARYYVEETGQLGEVPSNLQNYIDYQSLGRDMEIEGNYLVTSHGVF
                     EYCQ"
     gene            6991..7629
                     /locus_tag="IV334_001493"
     CDS             6991..7629
                     /locus_tag="IV334_001493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000248477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8943566.1"
                     /translation="MVNVFENLKLLKLDMKDKGWVIDSFYFRYKQQNYIVLVKLFEKE
                     EKVPEYALLKLEFLKENDFSDMLAVYANSVKLYTDTKTIREYFGIEYSSNLGDVLFQF
                     SQTLARFIPTEVSEKKNEDQKEAMCFSLSQSDSEDPRKKYCFSVRRNPLKGNGELGKR
                     SPFNDNKTRLYRPSLYERLGSDTNLSFRYSMNPDDEETDEGIIAKWTKNKIE"
     gene            7701..8096
                     /locus_tag="IV334_001494"
     CDS             7701..8096
                     /locus_tag="IV334_001494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_464632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAP8943567.1"
                     /translation="MKKIRSYTSIWSVEKVIYAINDLQLPFPITFTQMTWFIVSLFVV
                     ILFGSLPPLSLIDGALLKYLGIPIVLTGFMCQKTFDGKKPYGFLKSIITYALRPKVTY
                     AGKQIRLKKETMNESITIVRSEQLDGISD"
     gene            8080..10533
                     /locus_tag="IV334_001495"
     CDS             8080..10533
                     /locus_tag="IV334_001495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HAP8943568.1"
                     /translation="MAYPIKYMEDNLVFNHDGEVFAYYELIPYNYSFLSSDEKIQVHD
                     NFCQLIAQNRDGKIHALQLATESSIRATQEQSKKEITGRLKEIAYEKIDEQTEALISM
                     IGDNQVDYRFFIGFKLYLNEQEVSVKNVGKEIQKAFAEFFHGVNHKLMGDFVSMPMAE
                     IERFSKMEQLLESKLSRRFQIRKLEKDDFGYIIEHLYGQTGVPFDDYDYHLPKRYEDK
                     KVLVKKYDILKPTRCLIEENQRYLRIEQEDTTTYVAYFTINSIVGELDFPNSEIFYYQ
                     QQQFTFPVDTSMNVEIVANKKALSTVRNKKKELKDLDSHAWESDNETSTNVVDALESV
                     DELESNLDQTKESMYKLSYVVRVSAKNLDELKQRCDEVKDFYDDLSIKLVRPFGDMLG
                     LHNEFIPSSKRYINDYIQYVTSDFLAGLGFGATQMIGENSGIYVGYNLDTGRNIYLQP
                     SLASQGIKGSVTNALASAFIGSLGGGKSFSNNMLVYYSVLFGGQATIVDPKAERGEWK
                     ETLPDIAHEINIVNLTSENDNKGLLDPYVILKLPKDSESLAIDILTFLTGISSRDSEK
                     FPVLRKAIRSVTQSKQRGLLLVIDELRKEDTPISNSIADHIESFVDYDFAHLLFSDGS
                     VTQSISLEKQLNIIQVADLVLPDSNSSFEEYTTMELLSVAMLIVISTFALDFIHSDRS
                     IFKIVDLDEAWSFLQVAQGKALSMRLVRAGRAMNAGVYFVTQNADDLLDEKMKNNIGL
                     KFAFRSTDLVEIKKTLEFFGVDKEDENNQKRLRELENGQCLVSDIYGRVGVMQFHPIF
                     EELLHAFDTRPPVRNEVEE"
     gene            10530..12557
                     /locus_tag="IV334_001496"
     CDS             10530..12557
                     /locus_tag="IV334_001496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000192391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8943569.1"
                     /translation="MTQKQKKFLHYSWITLLCVGVILLLLGTLGNAVQATGLVDETID
                     TSNEYSKYGLNQYQLDYYVDNSWGWLPWNWSDGIGQSVMYGLYAITNFIWTISLYLSN
                     ATGYLVKEAYALDFISQTSQAIGENMQTIAGITPNGFSSSGFYVGFLLLFILIVGIYV
                     AYTGLVKRETTKVIRAVLNFVVVFILSASFIAYAPDYIAKINDFSKDVSTASLNIGTK
                     IVLPNTDSQGKDSVDMIRDSLFSVQVKQPWLLLQYGSTDVEALGEERVESLLSTSPDT
                     NNGEDRENVVIEEIEDHDNTYLTLPKTISRLGTVFFLFVFNIGISIFVFLLTGIMIFS
                     QVLFIIFAMFLPISFLLSMLPSFDGMGRHAITKLFNVIMTRAGITLIITVAFSISTML
                     YSLSASSPFFMIMFLQIVTFAGIYFKLGDLMSLFTLQSNDSQNMGRQVFRRPRQMMNR
                     QTRRFQRSMSKIFRGKAGSTKQKTMTTKQNQTNKANHTRQNERTTPKKETALSNVKKQ
                     QGGIGQRLGEKTAHVLDTKNRLVNKRKQAKQQIQATPTHVKYALHQGKENVTHNVTDF
                     KQSMADTKAQRKQQRMNQQEQKRKNIAQKRLELDKAKEKRRKENPSSPVQNNQQHVRK
                     RQQTVTPVLSQNTKRENHQRPLKKEQKQKTPPKQFNQRKLNKRTDKRGQQT"
     gene            12554..13576
                     /locus_tag="IV334_001497"
     CDS             12554..13576
                     /locus_tag="IV334_001497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000768374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase P60"
                     /protein_id="HAP8943570.1"
                     /translation="MKLKITVICSVVFSLFFFLLLFLVIFFADDTDSGENNKDSSIPQ
                     GGVTVSPEVLAHRPLIEKYGKEYGIEDYVSYILAIMQVESGGTAEDVMQSSESLGLPP
                     NSLSTEESIKQGVKYFSELLTSAEQQGVDIDSVIQSYNYGGGFLNYVRSHGKKYTYEL
                     AEQFSKEKSGGQKADYPNPIAIPVNGGWRYNYGNQFYVQLVSQYLTDTSPTEFDDETV
                     QVIMDEALKYEGFPYVFGGASPTTSFDCSGLIQWVYDKAGISLPRVAQDQYDATQEIS
                     MEEAQAGDLIFFHSTYNAGTYVTHVAIYLEGNRFYHAGDPIGYGDLSSRYWQDHLIGA
                     RRVIHN"
     gene            13593..14522
                     /locus_tag="IV334_001498"
     CDS             13593..14522
                     /locus_tag="IV334_001498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017650613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAP8943571.1"
                     /translation="MIIKIERKEKKEKPKKSKKPSKQRKVPMIKVGTHKKLTFVLWVL
                     LIGSVGFGIYKNFTAIDTHTVRETEIIKQQIVDTNQVESFVKSFAKDYFSWQQSQEAI
                     DKRNEKLTHYLTEELQVLNEEMIRKDIPTSSSVNDIQVWQVSQVNENTFEVLFSVEQV
                     ITEDKDKETISSSFHVVVHIDESDNMVIIKNPTMSKKPQKSDYQPKQLESDHTVDTET
                     MDEITSFLETFFQLYPTATEKELTYYVSNHVLPMINKEYVFEELVNPIFTRKDNQVIV
                     NVAVKYLDQETKATQISQFELILEKQDNWKIVK"
     gene            14767..14883
                     /locus_tag="IV334_001499"
     CDS             14767..14883
                     /locus_tag="IV334_001499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance determinant leader
                     peptide"
                     /protein_id="HAP8943572.1"
                     /translation="MILKYPENICMLCIPMVMHKNPSDKSIYHWDFYALLGF"
     gene            14899..15702
                     /locus_tag="IV334_001500"
     CDS             14899..15702
                     /locus_tag="IV334_001500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001820142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HAP8943573.1"
                     /translation="MKIINIGVLAHVDAGKTTLTESLLYNSGAITELGSVDKGTTRTD
                     NTLLERQRGITIQTGITSFQWENTKVNIIDTPGHMDFLAEVYRSLSVLDGAILLISAK
                     DGVQAQTRILFHALRKMGIPTIFFINKIDQNGIDLSTVYQDIKEKLSAEIVIKQKVEL
                     YPNMCVTNFTESEQWDTVIEGNDDLLEKYMSGKSLEALELEQEESIRFQNCSLFPLYH
                     GSAKSNIGIDNLIEVITNKFYSSTHRGPSELCGNVFKIEYTKKRQRLAY"
     gene            15877..16173
                     /locus_tag="IV334_001501"
     CDS             15877..16173
                     /locus_tag="IV334_001501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8943574.1"
                     /translation="MQIGVLKWLQFTKQKGVHFPMQFLEPKNKNAKSVDWEISEQVRV
                     IVKQYAEYAERTESEAVDEFLLNILDDKKFIEWIANKRSNKRIVEKMGIKDRVG"
     gene            16180..18132
                     /locus_tag="IV334_001502"
     CDS             16180..18132
                     /locus_tag="IV334_001502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017431446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insertion element protein"
                     /protein_id="HAP8943575.1"
                     /translation="MTKLKRLATKEDEIVDVKIPISNEELKDRAKQYDLLTPKRFATR
                     YNKMLFLPTSFKWNGSEYPIQYNYCINPFCCNFGKEQHKFKDVKGKPSRYKMTGSSKD
                     KGHKGMYCNDNPIGRGVSQNCTVTPLSNWSVVEEIKRLIEINSIQDVEPDYQFHKEGC
                     SEEESTPFNEPKQFYKRGKSRGKSQRYQCKACKKFTNVLPKREETTTYHQQKNTILPM
                     FAKMVVGRVSVSRTCDILGIGVGTYYHKLEWLYRRCLEFLERYETQPLQTKKFNEMWL
                     NTDKMHYYLNNVRKKGQGSKKYTGFEDLNMQTYIVVSAEVLSRYVFRSDVAYDWNISM
                     DELNEDTRKFKEDHLNTFSRKNDRLDWSYYPQEPSANDSENRNAYLHELGKITNRSRF
                     VDGLNVDAPYTTTAHYWLIKQMVNADEWRMISDDDFSIRNAFYRVFTKELRLSDAHHF
                     ICQVNKTKSRKQCLKEFGQAKAELLDWGDIRGFKTKFLRTLASHYLTELLTSHQFHEE
                     AISKDGERYRKYADNPIKHPLATKDKGFYSVDCRTDLSALEPNEIAKMLLNVNDHSTN
                     SFIQQIRRYISSLERPLTTARGDKKSYIYANFNPKYAQFAITILRTYYNFCRPFKSAD
                     KKVLTPAQRLGITDKQFDWKDIIYFK"
     gene            complement(18204..18701)
                     /gene="dfrG"
                     /locus_tag="IV334_001503"
     CDS             complement(18204..18701)
                     /gene="dfrG"
                     /locus_tag="IV334_001503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000868795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrG"
                     /protein_id="HAP8943576.1"
                     /translation="MKVSLIAAMDKNRVIGKENDIPWRIPKDWEYVKNTTKGHPIILG
                     RKNLESIGRALPDRRNIILTRDKGFTFNGCEIVHSIEDVFELCKNEEEIFIFGGEQIY
                     NLFFPYVEKMYITKIHHEFEGDTFFPEVNYEEWNEVFAQKGIKNDKNPYNYYFHVYER
                     KNLLS"
     gene            18931..>19122
                     /locus_tag="IV334_001504"
     CDS             18931..>19122
                     /locus_tag="IV334_001504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HAP8943577.1"
                     /translation="MKYILSFCTPKLNYLLAYIRLYSGVLHLRDSVRVSEKEKIKVTE
                     MYTSINGELCKIDRAYSGEI"
BASE COUNT         6567 a         3155 c         3699 g         5701 t
ORIGIN      
        1 attaaaaaat gaatttggaa tagaagttaa agattatttt attgacttag atttattaca
       61 gctaataaac agtgtgaaaa gaatgtatag tggagacgac aacggtgttg atttatacaa
      121 atttgttacc aaacatatat ttgttaatct taatattgac gagaaaaatt acccgtctat
      181 ttttaagcac tatctatttt ttattaacga agtagaagat cggataaaaa ataattcatt
      241 atctagtagt gtatctagtt ttaagaagat gtttaaatct cgagatggta tcacacttac
      301 tacagcacat aaagttaaag gaaatgagta tgatactgtt atcgctatta atcttctaaa
      361 cgggaaaatt cctcattgga atgaaatttt taatgtttct gataaaggaa ttagttcagc
      421 gagaaaacta ctatacgtaa tttgttcaag agcaaaaaat aatttatttc ttttctcaga
      481 ggtgggttat tttacgagta aaggagactt gctttatccc actcaattat tagataaaat
      541 agaatttgat tataatattt aaaataccta ggtagtcgtt gttttaaata tagtaggaat
      601 cggacgctca gtacggagcg tctatttttt tacccaattt tgaaaggaag tgaacttatg
      661 aaaataaaaa atcaagaatc aaaaggtcgc tctcccccct ttaaaaccat cacacaaata
      721 agattcattc aaacataaaa acagaaagga taggtaaaaa tatggaactt aaatatgtca
      781 ttcccaatat ggaaaaaaca tttggacaat tagaatatgc tggtgaaggg aacattgaac
      841 aacgtcgaat aaatggcaga ccaaccattc tctctcgtag ttacaattta tactcaacga
      901 ttcaacgagc agatgatatt gttgtcattc ttccctccga agctggcgag aaacattttg
      961 aatccgacga aaaagtcaaa ctcatcaacc cacatattac cgcagaaggt tataaaattg
     1021 gcaatcgagg atttaccaat tacattatgc acgcagacga tatggtgaaa gcataagaaa
     1081 ggaaaggtga aaaaatatga gattagcaga aggtattgtg attgaaaaag accagacatt
     1141 tggcacattg aaattttccg ccttgcgtcg tgaggttcgt atccagaatg aagatggtac
     1201 ggtttccgaa gaaattaaag aacgtaccta tgacttaaaa tcaaaaggac aaggacgcat
     1261 gatacaagtc agtatccccg cttctgttcc tttgaaagaa tttgactaca acgcagaagt
     1321 ggaattaatc aatcctgtgg cagataccgt agcgacggct acttttcgag gggcagacgt
     1381 tgattggtac gtgaaagcag acgatcttgt attaaaagga cagacaaaac ctcatcagaa
     1441 atataaaaaa gaagcgtccg aaagtgaaaa ataggtgttg cttatgacaa acacaccatt
     1501 gattttgaaa gaaattccga aggatgaagc catttctttt attcggcaat accattattc
     1561 aaagatactc cctagattgt gtaaatactt tctagggatt ttttcagaag aaaaattatt
     1621 aggcgttgtg gaattaggct ggggtacgca acccctacaa accatacgca aactctttcc
     1681 cgacagttcc cttcaaacca cagattattt agaaattgga aagatgtgtt ttcttccaga
     1741 aatgaatcaa accaattatt ttggcagtca agctctttca gctctaatta agtggttgaa
     1801 agaacatacg gactgtcatt ttttatatac actggcagat ggaattgagg gaaaatgtgg
     1861 ctatgtctat caagcgtcca acttctttta ctgcgggtat ttcaagacaa gtgtctatcg
     1921 tgataagcag tcatgggaaa agatacaccc tcgaagtgct cgccttttat tagaggaaaa
     1981 tgctcgcttt gaacaggtag agaaaaagca ttggctttcc caggcatttt gcgaatacaa
     2041 agggattgag aaaatcaatg gacgcatgtt tcgctatctc tatccgttga caaaagaagc
     2101 gaaaaaactg ttaggacaca ctctctatcg acgtcattat tatccgaaag aaaaaaattt
     2161 acgctttgaa aagcgaatcg cttatcagaa atatgaagcc atttctcaac caacttttga
     2221 taaacaggct cgtatctaca atacacaatt attttgatag gtggaaagga ggatttttat
     2281 gccattgctt catgacagag gagagcgaat caaagccaac gataagcact tggtctatca
     2341 ttttgtacta atttcattgc tggtagtttt tctagtcgtc ctactccctt ttcattggag
     2401 agcgttacaa tcggtgaact ggcaagcagt tcagttagat acatttttct atcaacttca
     2461 tacgccatac ttttggagca gtattctcac tgctcttttt gtatgcggac ttatcagctg
     2521 gctttattat cactatcgta tagattctat aaaacagtta gagcaccgtc aaaagttagc
     2581 tcgtatgatt ttagaaaatc attggtatga agccgaagaa actcaatcag agggattctt
     2641 taaagattta cccagcaaga aaaccaaaca acgaatccgc cattttccta aaatatatta
     2701 tcgaatgaaa gatggactaa tccatattca tgtagaaatc accttaggga aatatcaaga
     2761 tcagcttttg cacctagaaa acaaactaga aacaggattg tattgtgaat tagtggaaaa
     2821 aacgttgaaa gattcttatg tagagtatgt cctactttat gatactattg gcaatcgaat
     2881 tggcattgaa gacgtggtag ctcaagatgg acgtgtcaaa ttgatggaat cggtctattg
     2941 ggagttcgat tctctccctc acatgttaat tgctgggggc actggtagtg gaaaatccta
     3001 ttttattctc acattgattg aatctctcct gcatacaaat gcaaagcttt atattcttga
     3061 ccctaaaaat gctgatttgg cagacttagg ggcggtcatg aataatgtct attaccgtaa
     3121 agaagatatg ttggcatgta tcaatcaatt ttacgaagat atgattgatc gcagtgaaac
     3181 catgaaacaa caccctaact ataaaacagg agaaaattat gcttatcttg gtttgtctgc
     3241 caattttctt atttttgatg aatatgtggc aatgatggat atgctaggtc gtgaaagtac
     3301 caccgttatt cataaactca aacaaattgt aatgttagga cgacaagcag gcttcttttt
     3361 aattcttgcc tgtcaacgtc cagacgcaaa atattttagt gatgggattc gggacaactt
     3421 taacttccgt gtggctttgg gtcgtatgtc tgaaatgggc tttggtatga tgttcggaag
     3481 cgatacacaa aaacaatttt tcttaaaacc catcaaggga agaggttacg tggatacagg
     3541 aaaaagtgtg attagtgagt tttatacacc ccttgtacca aaaaggtacg atttcttagg
     3601 agaaatagga aaagtcattc aaaaaaagca atctgagcct gtaccgcacg aagtgaaagg
     3661 tacaggttca gacttgcttt gattggtgtg gcgttagcca cgccaatcct ttagccccct
     3721 ctatctaaca ggggggcaca aatacaactg aaaacaggca attctcatca accaatgttg
     3781 atggtacaaa ggattgcgga aaatatgaaa ggtgtaaact caagggtcat agtttacatc
     3841 ttttttagat tggaggtttg cgaatggaaa acgaaaaaat ttggattaag gaattgaaag
     3901 aaaagcgact ggcttatggc gtttctcaaa ataaactggc ggttgcttct catattacca
     3961 gaccttacct atcggatatt gaaacaggca aggcagtccc tactcaacag gtgaaagaag
     4021 atttactgaa cgctttggaa cgcttcaatc ccgataaccc attggagatg ttattcgact
     4081 atgtacggat tcgattccct accaatgatg tggctcaaat tatcggtgag gttttacgtt
     4141 tgaatatgga ttatatgcta cacgaagact ttggctatta ttcttatccc gaacactatc
     4201 gtttcggtga tattgttgtc ctagtctctc atgatgtaag taaaggtgtt ttattagaac
     4261 tgaaaggaaa aggctgtcgg caatttgaaa actttctatt ggctcaacat cggagctggt
     4321 atgatttctt tcaagattgc atggaacata aaggtatttt caaacgcttg gatttagcta
     4381 tcaacgataa aacagggatt ttgaatattc cagatttggc aaagaaatgt aaacaggaag
     4441 aatgcatttc cgtctttcga agctttaaaa attatcgcag tggtgaattg gttcaccgtg
     4501 atgaaaaacc atacatggga aacacacttt atattggctc attaaaaagt gaagtctatt
     4561 tctgtatata tgagaaagat tatgagcaat acgtgaaaaa tgatgttccc ttagaagacg
     4621 cagaagtaaa gaatcggttt gaaattcggt taaaaaatga ccgagccacc caagccatga
     4681 aagatttatt ggcacatcaa caggcggaaa aaacagcctt tgaaattatc aatcgctata
     4741 tccgctttgc agataaagac gacactaaac gtcggagtga ttggaaaact aatgaacgtt
     4801 gggaatggtt tatcggtaaa aatcgtggag cattacgcct aaccacacag ccagagcctt
     4861 attcttttga acgaacattg aactggttac atcatcaagt cgcccctaca ttaaaaatcg
     4921 caagcatttt agatgttctc aacggtacaa ccattatctc caccatgatt caggaagcca
     4981 aactgacaga aaaacatgag aaattaattg aacaacaaca tctagccatg gaggatttga
     5041 ttacatgagc cttttaacaa aagaattaaa gaagttaggg tttcagtgtg gcattgagtt
     5101 tcaagcgtat atacaaaata cagggaaata tacgtctctc attattgagg gaaaacggca
     5161 agctggtgat accatctata cctatgattt ttacaaggtg agattttata ataattatac
     5221 caatcgagta accgtctatg gagagcattt aacccctttt caattattaa ggcgtgtaaa
     5281 aagctatatc tattatcgag aaaaatatct taaggaaagg aggacaatca catgaatttt
     5341 gggcagaatt tgtacaactg gttcttggat aatgcacagt cattggtatt gttagcgatt
     5401 gttgtcattg gactgttttt aggttttaaa cgagaatttt caaaactcat tggcttcttg
     5461 attattgctc taatcgccgt cggacttgtg ttcaatgcag ctggcgtgaa agatgtgtta
     5521 ttaaacttat ttaaccgaat cattggtgct taacagatat ttttcatgat aaaataggaa
     5581 gtatatattt tgttatgaaa ggatttatga atatgtttac aaatgatcaa tttaaatctc
     5641 ttttaattaa aacattagaa agaaagagct cttcaaatta ttatgaagga ggtaacgaga
     5701 ttgtttctaa aatgaaaata tcagaggtaa ctttagatga gactgacgat tttacttatt
     5761 ataaaggata taaaaagggc tggaatacgc tttgcacata tcttaaaata agagttcctt
     5821 ttgatgattt agactttttt gaaagtcata aagatttgat tacccagaca gcaagctcca
     5881 tatatgataa acaaggtgat aatgtattag tggatactat tttagtacca cttccagaga
     5941 attacgaagt aattaacttt agtcagttga aaattagtga tgtggtttca caagctattg
     6001 aagacgctga gtcttttatg agtaatggtg aatatcaacg tgcctttgac agagttcaca
     6061 ctgcttttca tggatattta attgaaatac taaaaaaata tgaaattaca gttcctagag
     6121 acgaaaatct atctaaatta tatagtagga tacaacaatt aattgaaaaa gaaattcaac
     6181 ctactgaact tgctgatata gtgaagacaa cgataaggtc atcaaatgga atgattagct
     6241 ctcttaatga agcaagaaat aggcattcac ttgcacaccc taatacaaat attattggaa
     6301 agagagaagc aaaactaata ataggtattt caagtacagt tacagattat attagcggat
     6361 accttgataa gtagcgtcat taactgattg ctactttttt tatgactatt tttataaaaa
     6421 ggatgtgata agaattggaa atgaaagtct atgtggcaaa tttaggacgg tataatgaag
     6481 gtgaattagt cggagcatgg tttactccac caattgatga ggaagaaatg gcagaaagaa
     6541 tcggtttaaa tgaggactac gaagaatatg caatacatga ttttgagctt ccttttgatg
     6601 tggacgaata cacgccgatt agtgaaatca accgtctatg tgaagccatt caagaaatcg
     6661 aaggtacacc tatttataat gaactcaaag aaattcaagg catgtggttt agtagcttgg
     6721 aagaattact ggaaaacaaa gaggatattc attgttatag tgattgtgat tcgatggaag
     6781 atgtcgcccg ttattacgtg gaagaaacag gacagcttgg tgaagtccct tccaatttac
     6841 aaaattatat tgactaccaa tctttgggac gtgatatgga aatagagggt aattacctag
     6901 ttacctctca tggggtattt gaatattgtc agtaagttta acaatccgtg ttataattaa
     6961 attaataaat agcacggaga ggtataaatt atggtaaatg tatttgaaaa tcttaaacta
     7021 ttaaaactag acatgaaaga taaaggctgg gtgatagatt ccttttactt tcgttacaaa
     7081 cagcaaaatt atattgtact tgttaagcta tttgaaaaag aggaaaaagt tccagaatat
     7141 gctctgttaa aactagaatt tttaaaggaa aacgattttt cagatatgct agctgtctat
     7201 gctaattcgg taaaattata tacagacacg aaaacaataa gagaatattt tggtatcgaa
     7261 tacagtagta acttgggaga tgttttattt caatttagcc aaactcttgc tcgatttatt
     7321 ccgacagaag ttagtgagaa gaaaaatgaa gaccaaaaag aagccatgtg tttctcatta
     7381 agtcaaagtg attcagaaga tcccagaaaa aaatattgtt tttcagttag aagaaatcct
     7441 ttaaagggaa atggtgagct gggaaaacga agtcctttta atgacaataa aacgagattg
     7501 tatagaccca gcttatatga acgattaggg tcagatacca atttaagttt cagatattca
     7561 atgaatccag atgatgaaga aactgatgaa ggtattattg caaaatggac aaaaaataaa
     7621 attgaatagt taaagcattg aaagagcaat cacctatttt gattgttctt tttctatttt
     7681 tacggaaagg acgtgaagac atgaaaaaaa taagaagtta caccagtatt tggtcagtag
     7741 aaaaagtgat ttatgcgatt aatgatttac aactcccttt cccaattacc tttacgcaaa
     7801 tgacatggtt tatcgtttct ttgtttgtcg tcatactctt tggcagttta ccaccacttt
     7861 ctctgattga cggagcacta ctcaaatact tagggattcc cattgttctc actgggttca
     7921 tgtgccaaaa gacctttgac ggaaagaaac cctatggatt tctaaagtct atcattacct
     7981 atgctctacg ccctaaagta acctacgctg gcaaacaaat acgattaaaa aaagaaacaa
     8041 tgaatgaatc gattacgatt gtaaggagtg aacaactaga tggcatatcc gattaaatat
     8101 atggaagata atctcgtatt taatcatgat ggcgaagtat tcgcttatta tgaattaatt
     8161 ccctataatt acagtttttt aagttccgat gaaaaaattc aagttcatga taatttctgt
     8221 caattgattg cccaaaatcg tgatggcaag attcatgctc tgcaactagc aaccgaatca
     8281 agtattcgag ccacacagga acaatcgaaa aaagaaatca ctggacgatt aaaagagata
     8341 gcttacgaaa aaatagatga acagaccgaa gcgttaattt ctatgattgg tgacaatcaa
     8401 gtagactatc gcttttttat tggttttaaa ctctacctca atgaacagga agtatccgta
     8461 aaaaatgttg ggaaagaaat tcaaaaagca tttgcggaat tctttcatgg tgtgaatcac
     8521 aaattaatgg gcgattttgt ttctatgcct atggcggaaa tcgaacgctt ttctaagatg
     8581 gaacagttgt tagaaagtaa attatccaga cgctttcaga ttcgtaaatt ggagaaagat
     8641 gattttggtt atattattga acatctttac gggcaaacag gtgtcccttt tgatgactac
     8701 gactaccatt taccgaaacg atacgaagat aaaaaagtgt tggtaaagaa atacgatatt
     8761 ctcaaaccca ctcgctgttt aatcgaagaa aatcaacgct atttacggat tgaacaggaa
     8821 gataccacta cttatgtggc gtattttaca attaatagta ttgttggtga attggatttc
     8881 cctaatagtg aaatttttta ttaccagcaa cagcagttta ctttccccgt tgataccagt
     8941 atgaatgttg aaattgtcgc caataaaaaa gccttatcta ccgttcgcaa taaaaagaaa
     9001 gaactgaaag atttagatag ccacgcatgg gaaagcgaca acgaaaccag tactaatgtg
     9061 gtggacgctt tagaatctgt cgatgaatta gaaagtaatt tagaccaaac caaagaatct
     9121 atgtacaaac taagctatgt ggtacgggta tcagcaaaga atttggacga gctgaaacaa
     9181 cgttgtgatg aagtaaaaga tttttatgat gatctgtcta tcaaattagt acgacctttt
     9241 ggcgatatgc taggcttgca taatgaattt attccctcaa gcaagcgata tatcaatgac
     9301 tatattcaat atgtcacgtc ggattttttg gctggtcttg gtttcggtgc tacacaaatg
     9361 attggtgaaa attctggtat ttacgtcggt tacaatctcg atacaggcag aaatatttat
     9421 ctccaaccta gccttgcaag tcaaggaatt aaaggctctg taacaaatgc attagcgtcc
     9481 gcttttattg gttcattagg tggcggaaaa tcattctcta acaatatgct ggtctattat
     9541 tcagtactct ttggcggaca ggcaaccatt gttgacccaa aagctgaacg tggtgaatgg
     9601 aaagaaacct tacctgatat tgcccatgaa atcaatattg ttaatctcac gagtgagaac
     9661 gacaataaag ggttacttga cccttatgtg attttaaaac tcccgaaaga ttcagaaagc
     9721 cttgccattg atattctcac gttcttaaca ggtatttcca gccgagatag cgagaagttt
     9781 cctgtcctgc gtaaagctat tcggagtgta actcaaagca aacaacgagg gttgttattg
     9841 gtgattgatg aattgcgaaa agaagatacg cccatcagca atagtattgc tgaccatatc
     9901 gaaagctttg tcgattatga ttttgcccat ctgttattca gtgatggcag tgtcacccaa
     9961 tccattagct tggaaaaaca actcaatatt attcaagtgg cagatttggt cttacccgat
    10021 tccaatagct cttttgaaga atacactacc atggaattat taagtgtggc aatgctgatt
    10081 gtcattagta cctttgcttt agactttatc cactctgacc gttccatttt taaaattgtt
    10141 gaccttgatg aggcttggag ttttttacaa gtggcacaag gaaaagctct atctatgcga
    10201 ttggttcgag caggacgtgc catgaatgca ggggtctatt tcgttactca aaacgcggac
    10261 gatttactag atgaaaagat gaagaataat attggcttga aatttgcctt tcgttccact
    10321 gacctagtgg aaatcaaaaa gaccttagaa ttttttggtg tggacaagga agatgaaaac
    10381 aatcagaaac gcttacgtga attagaaaat ggacaatgtt tagtttcaga catctatggt
    10441 cgtgtagggg ttatgcagtt tcacccgatt tttgaagagt tgcttcatgc ctttgatacc
    10501 agaccgcctg tgcgaaatga ggtggaagaa tgacacaaaa acaaaagaaa tttcttcact
    10561 atagttggat aacgctcctt tgtgtgggcg ttattttact tttattaggc acgctgggca
    10621 atgcagtaca agcgacaggc ttagttgatg aaaccattga tacaagcaat gaatactcca
    10681 agtatggttt gaatcaatat cagcttgatt attatgtgga caatagctgg ggctggcttc
    10741 catggaattg gagtgatggc ataggacaat cggtgatgta tggattgtat gccattacta
    10801 attttatttg gacaatttct ctttatctat cgaacgccac aggttatttg gtgaaagaag
    10861 cctatgcctt agatttcatt agtcaaacat cccaagcaat tggcgaaaac atgcagacga
    10921 ttgcaggcat tacaccgaat ggcttttcca gttcagggtt ttatgtcggt ttcctccttc
    10981 tttttattct cattgttgga atctatgttg cctatacagg attggttaag cgagaaacga
    11041 cgaaagtgat acgagctgtt ttaaactttg tcgtggtgtt tattctatcg gcttccttta
    11101 ttgcgtatgc acccgattat attgcgaaaa tcaatgattt ttccaaagat gtcagtaccg
    11161 ccagtctgaa tattgggaca aaaattgttc ttcctaacac ggatagtcaa gggaaagaca
    11221 gtgtggacat gatacgtgac agcttattct ctgttcaagt aaagcaacca tggctattgc
    11281 ttcaatatgg ctccactgat gtagaagcgt taggggaaga acgtgtggaa agtttattgt
    11341 ctaccagtcc agatacaaac aatggtgaag atagagaaaa tgtagtgatt gaagaaattg
    11401 aagatcatga caatacctat ttaaccctac cgaagacgat ttccagatta ggaacagtct
    11461 tctttttgtt tgtattcaat attggtatct cgatttttgt tttccttcta acaggaatca
    11521 tgattttttc gcaagtgcta tttattattt tcgctatgtt tttgccaatc agtttcctat
    11581 taagtatgtt gccgagtttt gatggcatgg gcaggcatgc cattaccaag ttatttaatg
    11641 tgattatgac aagggctggt attaccttga ttattaccgt tgcctttagt atttccacta
    11701 tgctttattc gctgtcagca agttctccct ttttcatgat tatgttttta caaattgtga
    11761 cctttgcagg gatttatttt aaactgggcg atttaatgag cctattcact ttacaaagca
    11821 acgactctca aaacatgggc agacaagtgt ttcgcagacc tcgtcaaatg atgaatcgac
    11881 aaaccagaag atttcaacgc agtatgagta agatttttcg tggaaaagca gggtcaacga
    11941 agcaaaagac aatgacaaca aagcagaatc aaaccaataa agctaaccat actcgtcaaa
    12001 atgaacggac tacaccaaag aaagaaacag cactttccaa tgtgaaaaag caacaaggcg
    12061 gtatcgggca acgattaggt gaaaagacag cccatgtatt ggacacgaaa aatcgtctag
    12121 tcaataaaag aaagcaggca aaacaacaga tacaggcgac accaacccat gtcaaatatg
    12181 cccttcatca aggaaaagag aatgtcactc ataatgtaac agactttaaa caatctatgg
    12241 cagataccaa agctcaacgc aaacaacagc gtatgaacca gcaggagcaa aaacgaaaga
    12301 acattgctca aaagcgtttg gagctggata aagcaaaaga aaaaagacga aaagaaaacc
    12361 catcatctcc tgttcagaat aaccagcagc atgttcgcaa gcgtcagcaa accgtcacgc
    12421 ctgttttgtc acaaaacact aaacgtgaaa atcatcagcg accattgaaa aaagagcaaa
    12481 aacaaaagac accaccgaag caattcaatc agaggaagct aaataaaaga acggataagc
    12541 gaggacaaca aacatgaagc taaaaataac agtgatttgt agtgtggtct tctctctctt
    12601 cttttttctc ttattattcc tagtcatttt ctttgcagat gatacggaca gtggcgaaaa
    12661 caacaaggat tcttctattc cacaaggcgg tgtgaccgtt tcaccagaag tgttggcaca
    12721 ccgtccgttg attgaaaaat atggtaagga atatgggatt gaagactatg tttcttatat
    12781 cttagccatt atgcaagtgg aatcgggcgg tacagcagaa gatgttatgc aaagttccga
    12841 atctttgggt ttaccgccca acagtttgag taccgaagag tccatcaaac aaggggttaa
    12901 atatttcagt gaattattaa ccagtgcaga acaacaagga gtggatatag attctgttat
    12961 ccaatcttat aattatggcg gtggcttttt aaattatgtg aggagtcatg ggaaaaaata
    13021 tacctatgaa ttagccgaac agttttctaa agagaagtcg ggtggtcaaa aagcagacta
    13081 tcccaatcct atagccatac ctgtgaatgg tggttggcga tataactatg gaaatcagtt
    13141 ttatgttcag ttggtttcac aatacctaac cgacacttcc ccaacagaat ttgatgatga
    13201 aaccgtgcaa gtcattatgg acgaagcctt gaaatacgaa gggtttccct atgtctttgg
    13261 aggggcgtcg cctaccacct cttttgattg tagtggctta atacaatggg tgtatgacaa
    13321 agctggcatt tctctccctc gtgtcgccca agatcagtat gacgctacgc aagaaatctc
    13381 tatggaagaa gcccaagcag gcgaccttat cttctttcat tcaacctata atgcaggcac
    13441 atatgtgact catgtggcga tatatttaga gggaaatcgc ttttatcatg caggcgaccc
    13501 aatcggttat ggcgacttaa gcagtcgtta ttggcaagac catctgattg gggctagacg
    13561 tgttattcat aactaagaaa ggaatgaatc aaatgattat aaaaatcgag cgaaaagaaa
    13621 agaaggagaa accaaagaaa tctaaaaaac cgagcaagca aagaaaagtt ccgatgatta
    13681 aagttggcac gcataagaaa ttaacctttg ttttatgggt tttattgatt ggtagtgtcg
    13741 gatttggaat ctataaaaat tttactgcca ttgacactca tactgtccgt gaaacagaaa
    13801 tcattaaaca gcagattgtc gataccaacc aagtagaaag ttttgtgaaa tcctttgcga
    13861 aagactactt ttcgtggcaa caatcccaag aagccattga taaacgaaat gaaaaattga
    13921 cccattattt gacggaagaa ttgcaggtat taaatgaaga aatgattcgt aaggatattc
    13981 ctactagttc tagtgtgaac gatatacagg tgtggcaggt ttctcaagtg aatgagaaca
    14041 cctttgaggt actgttttct gttgaacaag ttattaccga agacaaagac aaggaaacta
    14101 tatcgtctag ctttcatgtg gttgtccata tagatgaatc agataatatg gttattatca
    14161 aaaatccgac aatgagtaag aaacctcaaa aatcggacta ccaacccaaa cagctagaaa
    14221 gtgaccatac agtagataca gaaacgatgg acgaaatcac tagcttttta gaaacgttct
    14281 tccagctcta tcctaccgcc acagaaaaag aattgactta ctatgtaagt aatcatgtgt
    14341 tacctatgat taacaaggaa tatgttttcg aggaattggt gaatcctatc tttaccagaa
    14401 aagataacca agtaatcgtg aatgtcgctg tgaaatattt agatcaagaa acaaaggcaa
    14461 cccaaatctc gcaatttgag ttgatattag aaaaacaaga taattggaag attgtaaaat
    14521 aacaaatatt ggtacatgat tacagatact ttgtaatcat gtactctttt tgataaaaaa
    14581 ttggagattc ctttacaaat atgctcttac gtgctattat ttaagtgact atttaaaagg
    14641 agttaataaa tatgcggcaa ggtattctta aataaactgt caatttgata gcgggaacaa
    14701 ataattagat gtcctttttt aggagggctt agttttttgt acccagttta agaatacctt
    14761 tatcatgtga ttctaaagta tccagagaat atctgtatgc tttgtatacc tatggttatg
    14821 cataaaaatc ccagtgataa aagtatttat cactgggatt tttatgccct tttgggtttt
    14881 tgaatggagg aaaatcacat gaaaattatt aatattggag ttttagctca tgttgatgca
    14941 ggaaaaacta ccttaacaga aagcttatta tataacagtg gagcgattac agaattagga
    15001 agcgtggaca aaggtacaac gaggacggat aatacgcttt tagaacgtca gagaggaatt
    15061 acaattcaga caggaataac ctcttttcag tgggaaaata cgaaggtgaa catcatagac
    15121 acgccaggac atatggattt cttagcagaa gtatatcgtt cattatcagt tttagatggg
    15181 gcaattctac tgatttctgc aaaagatggc gtacaagcac aaactcgtat attatttcat
    15241 gcacttagga aaatggggat tcccacaatc ttttttatca ataagattga ccaaaatgga
    15301 attgatttat caacggttta tcaggatatt aaagagaaac tttctgccga aattgtaatc
    15361 aaacagaagg tagaactgta tcctaatatg tgtgtgacga actttaccga atctgaacaa
    15421 tgggatacgg taatagaggg aaacgatgac cttttagaga aatatatgtc cggtaaatca
    15481 ttagaagcat tggaactcga acaagaggaa agcataagat ttcagaattg ttctctgttc
    15541 cctctttatc atggaagtgc aaaaagtaat atagggattg ataaccttat agaagttatt
    15601 actaataaat tttattcatc aacacatcga ggtccgtctg aactttgcgg aaatgttttc
    15661 aaaattgaat atacaaaaaa aagacaacgt cttgcatatt aagtagttca gttttggagt
    15721 acaaaaagtg aaaatgtaaa cctaaatgag tatcaaattg caactgaatt tgatactcgt
    15781 tttttattgt taaatcaacg tttgttgtta atttacacca caaaatgtaa gttaaaatgt
    15841 atataaaaat gcgaacgtga aataattatt gtaggaatgc aaattggtgt gttaaaatgg
    15901 ttacaattta ctaagcaaaa aggggtgcat tttcctatgc aatttcttga gccaaaaaat
    15961 aagaacgcta agtctgtgga ttgggaaata tcagagcaag taagggtgat agttaagcaa
    16021 tacgctgaat atgcagagcg tactgaaagc gaagcagtgg atgaattctt attgaacatt
    16081 ttagacgata agaagtttat tgaatggatt gccaacaagc gaagtaataa gcggattgtt
    16141 gaaaaaatgg gtatcaaaga tagagtgggt tgatagacaa tgaccaagct taaaagatta
    16201 gccacgaagg aagatgaaat tgtagatgtt aaaattccga tttcaaacga agaattaaaa
    16261 gatagggcta aacaatatga tttattaact ccaaaacgat ttgcaacaag gtataacaaa
    16321 atgttgttct tgccaacgtc ttttaaatgg aacggttctg aatatcccat tcaatataat
    16381 tactgcatca atccgttttg ttgtaatttt gggaaagaac agcacaaatt caaagatgta
    16441 aaagggaaac caagtcgata taaaatgact ggtagttcca aagataaagg tcataaaggt
    16501 atgtactgca atgacaatcc tattggcaga ggtgtatcgc aaaactgtac agtaacccct
    16561 ttatctaatt ggtctgttgt tgaagaaatt aaacgcctta tagaaatcaa tagcatccaa
    16621 gatgttgagc cagattatca atttcataaa gaaggctgct cggaagaaga atcaacccca
    16681 ttcaatgagc cgaagcaatt ttacaaaagg gggaaaagta ggggcaaatc tcaacgctat
    16741 caatgtaagg cgtgtaagaa gttcactaat gttctaccaa aaagagaaga aaccactact
    16801 tatcaccaac aaaagaacac cattcttcca atgttcgcta agatggttgt tggaagggtt
    16861 tcagtcagtc gtacttgtga tattttagga attggagttg gaacctacta tcataagctg
    16921 gagtggctgt ataggcgttg tttggagttt ttagaacgat atgagacaca accccttcaa
    16981 acaaagaaat ttaacgagat gtggcttaat acggacaaga tgcactacta cctaaataac
    17041 gtccgtaaga aaggacaagg ttcaaagaaa tatacggggt ttgaagacct taatatgcaa
    17101 acgtatatag ttgtatctgc tgaggtctta tcaagatatg tgttccgttc tgatgttgcc
    17161 tatgattgga atatttcaat ggatgaatta aatgaagata caagaaaatt caaagaagac
    17221 catctcaata cttttagtcg taaaaatgat aggttagatt ggtcttatta tccacaagaa
    17281 ccatccgcta acgatagcga aaataggaat gcttatcttc acgaattagg gaaaattact
    17341 aataggagta gatttgtcga tggtttaaat gttgatgctc cttatactac aactgctcac
    17401 tattggttaa ttaaacaaat ggttaatgcc gacgaatggc gaatgattag tgatgatgac
    17461 ttctctatta gaaacgcctt ttatagagta ttcacgaaag aattaaggtt gagcgatgca
    17521 catcatttta tttgccaagt taacaagacg aaaagcagaa aacaatgctt aaaagaattc
    17581 ggacaagcaa aagcagaact gctcgattgg ggagatatca gaggattcaa aacaaagttt
    17641 ctacggactt tggcatctca ttatctaact gaattactaa caagtcatca attccacgaa
    17701 gaggctatta gtaaagatgg agaaagatat cgcaaatatg ccgataaccc tatcaaacat
    17761 ccattagcca ctaaggataa aggattttat tcagttgatt gtagaacgga tttatctgct
    17821 ctggaaccaa acgaaatcgc aaagatgcta ttaaatgtta atgaccattc gaccaatagc
    17881 tttattcaac agataagacg ttatatatca tctcttgaaa gaccattaac aacagctcgt
    17941 ggtgataaga agagctatat ttacgctaac ttcaatccga agtatgctca atttgcgatt
    18001 acaattctaa gaacttacta caacttttgt cgccctttta aatcagcgga taaaaaggtt
    18061 ttgacacctg ctcaaagatt agggattaca gataaacaat ttgattggaa agatataatt
    18121 tatttcaaat gacaaaaaga gcttggagta atcccaagct cttgtgcttt atctttactg
    18181 ttcttcttca attaacgcac ccgttaactc aataagtttt ttctttcata tacatgaaaa
    18241 tagtagttat acggattttt atcattcttt atcccttttt gggcaaatac ctcattccat
    18301 tcctcataat tcacttctgg aaaaaaagta tctccttcga attcatgatg tatttttgtg
    18361 atgtacattt tctcaacata agggaaaaac aaattataaa tctgttctcc tccgaaaata
    18421 aaaatttctt cttcgttttt acataactca aaaacatctt ctattgaatg aacaatttca
    18481 caaccattaa aggtaaaccc cttatctctc gtcagaataa tatttcttct gtcaggtaag
    18541 gctcttccga ttgattcaag gttcttccta cctaatatta tcggatgtcc ctttgtagta
    18601 tttttaacat attcccagtc cttgggaatc ctccaaggaa tgtcattctc tttgccaatc
    18661 actctattct tatccatcgc agcaatcaaa gaaactttca tttataacac agccttttta
    18721 actaatttac catctaattt cttatttcta tcaaaaaacc ttttctcatt atcctgcccg
    18781 ttagttgaat aagcaatttt ttctataagt tatattatca taataccaca aaatcacaaa
    18841 ttttcattca attatcattt tcaattgcat tttataaatc attttgtatg taatttggta
    18901 tgtaagttaa aatgaaaaaa gtagtccatt ttgaagtaca ttttgtcatt ttgtactcca
    18961 aaactgaact acttacttgc atatatacgc ctttatagtg gagtactaca tttacgagat
    19021 tcggttagag tatcagaaaa agaaaaaata aaagttacag aaatgtatac ttcaataaat
    19081 ggtgaattat gtaagattga tagagcttat tctggagaaa tt
//
LOCUS       DACDKM010000046        18990 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20834_2016
            SAMN12640370-rid9891663.denovo.046, whole genome shotgun sequence.
ACCESSION   DACDKM010000046 DACDKM010000000
VERSION     DACDKM010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640370
            Sequence Read Archive: SRR10040891
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 18990)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18990)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742835.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742835.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:04:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,012
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 29 of 75
            Pseudo Genes (incomplete)         :: 33 of 75
            Pseudo Genes (internal stop)      :: 19 of 75
            Pseudo Genes (multiple problems)  :: 5 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18990
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640370-rid9891663.denovo.046"
                     /isolate="E20834_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(83..604)
                     /locus_tag="IV334_001505"
     CDS             complement(83..604)
                     /locus_tag="IV334_001505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HAP8943578.1"
                     /translation="MDISVIDATKTNTQKGILYGSSNAPKKMVEFINLACPYCRQWFE
                     ESYELLEEAVQSGQLQRVIKLFDKEKESLQRGNVMHRYLTISDGQKAIKEIKQIFDTQ
                     DEWKHLSLQEVADFAVDKLKLTEQKDEQLSQAVINEAEQAHIRFVPTVILGKEIFDES
                     ISIEELKELIQTK"
     gene            803..4495
                     /gene="nifJ"
                     /locus_tag="IV334_001506"
     CDS             803..4495
                     /gene="nifJ"
                     /locus_tag="IV334_001506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HAP8943579.1"
                     /translation="MRKMKTMDGNTAAAYISYAFTEIAAIYPITPSSTMAELVDQWSA
                     AGKENVFGQPVKVVEMQSEAGAAGVVHGSLKTGALTTTYTASQGLLLMIPNMYKIAGE
                     LLPSVFHVASRAVTTNALNIFGDHGDVMAARQTGFAMLAESSVQEVMDLAPVAHLASI
                     EGEVPFLNFFDGFRTSHEIQKIEVLEYEELAPLINQEKLASFRKRGMNPNHPTVSGTN
                     QNPDIHFQQRETINPYYDKLPEIVQKYMKEINRLRGTSYDLVTYYGADDAEEVIVSMG
                     SVAQTIEQTIDYLNANGRKTGFLNIHLYRPFPVETFVQKLPHTVKTIAVLDRSKEPGA
                     GGEPLLLDVQSALYDSELRPAVIGGRYGLGSKDVTPDQISAVFDELKKDPSIRKKRFT
                     IGIVDDVTYQSLEPKESLDLTEPQTFQAKFWGFGSDGTVGANKSAIKIIGDHTDKYAQ
                     GYFYYDSKKSGGLTVSHLRFGDTPIRSAYLVEHADLVACHTPAYLHSYDLVKGLKPGG
                     IFLLNTLWSDEQLETHLPLKLKRYLAENNIRFYTINAMRLAQEVGLGRRINTAMETAF
                     FKLADIIPFDEVLPLLKEEALKSYGHKSMAIVEKNIQAIDRTTELLHQVAIPDHWRTL
                     EMPEKINKRNTGYIQEILEPVNAQEGNQLSVGTLVANGMTTGEMPLGTASMEKRGVAL
                     EVPEWISDRCTMCNECAFVCPHAAIRPFLADEEEMEEAPEGFIVREMRGADGVKYRIQ
                     VSVEDCTGCGLCVEACPAKEKALVMKPYEEQKEQAVNWAFAMTLRQKANPVKGKNTVL
                     STQFNQPLLEFSGACSGCGETPYVKLLTQMFGDRMMIANATGCSSIWGGASPASPYTT
                     NECGQGPAWSNSLLEDNAEFGYGMLLAAQTRREALASSIKEVLPKVSEELRELMEDWL
                     VHMFESEGTQQRAAKLQAAIESERSIFPELEDIYKRKDLFVKNSQWMIGGDGWAYDIG
                     FGGIDHVLASGADVNILVLDNEVYSNTGGQTSKATPASAIAKFSASGKYASKKDLGMM
                     AMTYGNVYVAQIASGANQMQTIKAFEEAEKYPGPSLIIAYTPCITHGLLGGMKESLKE
                     AKEAVSSGYWSLYRYNPTMVEKNKNPMTLDYKKPDFSAMPDFMRKQVRFSSLDNANPN
                     LAKALFEKTVHDAKTRFYNYAALTGELEKIKKRLEPEESIDVPVKTRKEKTTDLEAEE
                     RRAARRAARAERRKKMEQATKEKKEK"
     gene            4684..5571
                     /locus_tag="IV334_001507"
     CDS             4684..5571
                     /locus_tag="IV334_001507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00159 family protein"
                     /protein_id="HAP8943580.1"
                     /translation="MSFQIGELFNLDYWQQMFSGDLFSLNFLVNILDILVVWYLVYKL
                     IQLLRGTKAIQLLKGVAIFIVIRIVAEIIGLHTLSWLMNQVITYGVIAAIVIFQPEIR
                     RGLEHLGRSSLFKQSKSEQQEDEKMILSFDKAIQYMSKRKIGALITIERHTGLDEYIE
                     TGIALDADITGELLINIFIPNTPLHDGAVIVKEGKIAVASAYLPLSESMLIPKEFGTR
                     HRAAVGISEVSDAITIVVSEETGDVSITLDNELMAGLSQQEYLAILRRELIAEEKTKN
                     KKNVIQSFLDGMTKGGDRK"
     gene            5568..6737
                     /locus_tag="IV334_001508"
     CDS             5568..6737
                     /locus_tag="IV334_001508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8943581.1"
                     /translation="MISKEKRTSILYSFIALLFSIALYFNANGQSVPNTLSGNEAYNQ
                     TVTGVPIKISYDSKRYYIHGYENAVTVKLSSANRVQLNAEANEDTRMFRVTADITKLG
                     EGTHEVPLKIQNLSSAVTAKIEPSTITVTVEKKVIKDFDVETLIPSTFTPSGYELDDT
                     SVSPKKVSITTGDKTLAEIKRVVADVDASMVTDDGINSEVPIQALNAAGEVLSIVSDP
                     VQVTVRADSIKPSKSVRLYGIQQGTPAAGVESYDFSFSKLEAEVSGSSDLLASIGDSI
                     AVPINVSGINHRTTRKIEIPVEEGLSIKPESVSVEITPVLQTSSSSSASTSSRTQENT
                     STSRSISSADSGTEPSTTTISSSSESGTSSSTTSTEETSETSESTQMSRTNSSEN"
     gene            6761..8116
                     /locus_tag="IV334_001509"
     CDS             6761..8116
                     /locus_tag="IV334_001509"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HAP8943582.1"
                     /translation="MGKYFGTDGVRGEANKELTPELAFKLGRCGGYVLSQHEEEGKRP
                     RVLVGRDTRISGQMLEEALISGLLSVGIEVFQLGVISTPGVAYLTRLQKASAGVMISA
                     SHNPAQDNGIKFFGADGFKLVDDQELEIEALLDAEVDELPRPSAEGLGTVEEFPEGLL
                     KYSQFLVQTINGDLSGLTICVDAANGATSTSVNRLFADLETDFYTMGTNPDGLNINDG
                     VGSTHPERLAEMVVEKGADAGLAFDGDGDRIIAVDELGNIVDGDKIMFICAKYLAEKN
                     RLKKDTIVTTVMSNLGFHKAVEGIGLKDVVTQVGDRYVVEEMRKNDYNFGGEQSGHMI
                     FLDFNTTGDGMLSGIQLLNIMKQTGKKLSELAAEVTIYPQKLVNIRVSDKNGAMDVPA
                     IKKVIEEAEAEMGSEGRILVRPSGTEPLLRVMAEAPTDEKVNYYVDKIAAVVKEEIGL
                     D"
     gene            complement(8188..9993)
                     /gene="glmS"
                     /locus_tag="IV334_001510"
     CDS             complement(8188..9993)
                     /gene="glmS"
                     /locus_tag="IV334_001510"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HAP8943583.1"
                     /translation="MCGIVGMIGLKNVTPGLIDGLEKLEYRGYDSAGIFVSDGTTDYL
                     VKAQGRIQNLKNKINIDTTGNIGIGHTRWATHGQPSEENAHPHTSQSGRFVLVHNGVI
                     ENFEELKIAYLANDHFIGETDTEIIAHLIETFAKDTTTQEAFLKALRVIKGSYAFALI
                     DRTAPDVIYVAKNKSPLLIGLGDGFNVIASDAMAMLAHTKEFVEIEDEEMVTVTSEKV
                     IIQNFAGDIVERSSFEAQVDASDIEKGTYPFYMLKEIDEQPIIMRRIAQKYVTEDNRV
                     SLDQKLVDTLSDSDRIYIVACGTSYHAGLAGKQTLEKLTQIPVEVHLASEFGYNTPLL
                     SKKPFFIFLSQSGETADSRQVLVKINRLGYPSLTITNVAGSTLSREASFTLLLHAGPE
                     IAVASTKAYTAQIAVLTLLAKAIGDKKEFETSLVFDITHELSLVANAMESVIAQKDYL
                     EELAEVYLSDTRNAFYIGRGADYNVSLEAALKLKEISYIQAEGFAAGELKHGTIALIE
                     EGTPVIGIISEEVTGAHTRGNLKEVESRGAKTLVIVSEGLEKESDQLVLPTVHPYLTT
                     LTTVVPTQLLAYYATLLRGFDVDKPRNLAKSVTVE"
     gene            10402..10692
                     /locus_tag="IV334_001511"
     CDS             10402..10692
                     /locus_tag="IV334_001511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8943584.1"
                     /translation="MDSKIEIMTLGMLKKQLSKFEASAGVSDDTKIFLDTGWDSIQEI
                     APDALEVAQAREFTVEDELTKESFSGYAREEKAERFDTSEQSETVIVIKNLY"
     gene            10705..11730
                     /gene="sppA"
                     /locus_tag="IV334_001512"
     CDS             10705..11730
                     /gene="sppA"
                     /locus_tag="IV334_001512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HAP8943585.1"
                     /translation="MNKRRWAAVLIAAGLFVVSLVSASLTKPQENDQIEGLNSWLYGD
                     EELAPNVLEEGDSTQKIVKLSVDGTIAAGGSSGLFASEGYNHKNFMEQLKAIEEDPTV
                     KGIFLEVNSPGGGVYESAEIAKKLDTIRKEHDIPMYVSMKNMAASGGYYISAQADKIF
                     ATEETVTGSIGVIMSGLNYSGLLEKLGVEDTTVKSGALKDMGSGTRPETKEEEAVLQA
                     YIDSAYSRFVAIVSEGRNKSEEEVKKIADGRIYDGVQAKEVGLVDELGFPEDALKAIR
                     KEQQLEDAELVEYSSTPSGFASTWLGSKLAEFQGLKPSETSQILSVLENLGTAESPKA
                     MYYYGGE"
     gene            11733..12392
                     /locus_tag="IV334_001513"
     CDS             11733..12392
                     /locus_tag="IV334_001513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HAP8943586.1"
                     /translation="MSELSNENKEQEKDKQQQPAVGPDSNFSQKVLSSFEREPLTPEE
                     IKKKQRKWKKYTDTRNEPPKANDFPKYFYAGFFIRMFAFLVDLLCIGAITRLTLGIAK
                     NLGWLNFSDSYLSLYGFTALLIYLAYFILLTKFNHGQTIGKMIFGIRVISFDEEELSW
                     STVLVREGACRFILKFPLLMLGYLPAVFNQKKQHLGDFFTNTSVVTINLIKAFNQEVN
                     S"
     gene            12549..13481
                     /locus_tag="IV334_001514"
     CDS             12549..13481
                     /locus_tag="IV334_001514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8943587.1"
                     /translation="MKPIDFPEKYENLMRVAQQALANQQYQQAKELFQRAYELKESFE
                     ANSLLVFCLYELDEKKEALKQALLHEKQYLQNEEFAEFYFDLLISTQDFLYARKLIAS
                     TDFYEAFEQRVIEKIQFAEELSGQMERQKIKALHNKSEELPSLEPARQLASIDEIEQL
                     PYHEFIQTASKLIVSPEVHILARAKLLETLRQLNECHPVFYLTIEEKLVKVIPKDLPK
                     PQQQASYQQLCVFSDRYGNEDALLSSVLKEEFTLQSAIVYPVYDTYIEDPKRWFQLTV
                     EACTGKTMYEGTEDEKQDFLKKREKILQQMIFFH"
     gene            13635..14924
                     /locus_tag="IV334_001515"
     CDS             13635..14924
                     /locus_tag="IV334_001515"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HAP8943588.1"
                     /translation="MTATFEKKGTNDGVLKFTIAQDEIQKGLTTAFNKVKKSLNVPGF
                     RKGKVSRQVFNRMYGEEALYEDALNAVLPEAYEAAVKEAGIDPVSQPKIDVESMEKGQ
                     DWVITAEVTVKPEVKLGQYKDLVVEKQDREVTDADVDARIKREQEAQAELVIKEDAAA
                     ENGDTVVIDFEGFVDGEAFDGGKGENYSLELGSGSFIPGFEDQLVGHKSGESLDVNVT
                     FPEDYQAEDLAGKEAVFKVTIHEVKAKEVPELDDEFAKDVDDEVSTLDELKEKYRKEL
                     VETKEKAADDAKDEAAIRMAVENAEIVELPHVMVHDEVHRSMDEFLNNMQRQGISPEM
                     YYQLTGSTEEDLHKQFEGEAEMRTRTNLVIEAIAAAENLQATEEEINAEIQELSETYN
                     MPIEQIKRVLTEDMLEHDVTMKKAIELITGTATEAKE"
     gene            15171..16421
                     /gene="clpX"
                     /locus_tag="IV334_001516"
     CDS             15171..16421
                     /gene="clpX"
                     /locus_tag="IV334_001516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HAP8943589.1"
                     /translation="MYDNPSSNETVRCSFCGKTQEEVKKIVAGPGVYICNECIDLCKE
                     IIDEEFYEEAVREFTEVPKPLEILEVFNNYVIGQDRAKRSLAVAVYNHYKRVNQQAQE
                     NTDDVELQKSNICLIGPTGSGKTFLAQTLAKTLNVPFAIADATSLTEAGYVGEDVENI
                     LLKLLQSADYNVELAEKGIIYIDEIDKIARKSENVSITRDVSGEGVQQALLKILEGTV
                     ASVPPQGGRKHPHQEFIQIDTTNILFIVGGAFDGIETIVKNRMGEKTIGFGTQNQKLS
                     EDESVMQHIIPEDLLKFGLIPEFIGRLPVMAALEKLTTDDLVRILTEPKNALVKQYQK
                     LLSLDNTELEFEPEALRAIAKKAIERNTGARGLRSIIEEIMMDVMFDIPSNENIEKVI
                     ITKEAAELSGEPTVIYKDSEKKAG"
     gene            16504..17091
                     /locus_tag="IV334_001517"
     CDS             16504..17091
                     /locus_tag="IV334_001517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihA family ribosome biogenesis GTP-binding
                     protein"
                     /protein_id="HAP8943590.1"
                     /translation="MKVHQAEIIISAVAPKQYPDTQLPEIALAGRSNVGKSSFINTLI
                     DRKNLARTSGKPGKTQTLNFYLIENALHFVDVPGYGYAKVSKTERAKWGKMIETYLTT
                     RDQLRAVVSLVDLRHKPSQEDIQMYQFLKYYDIPVIVVATKADKIPRGKWNKHESEIK
                     KALDFDRNDTFILFSSVTKQGKEEAWAAIEQAIES"
     gene            17456..18784
                     /locus_tag="IV334_001518"
     CDS             17456..18784
                     /locus_tag="IV334_001518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8943591.1"
                     /translation="MIGEDKLLMTIQLTDLNEEEKKQEISDWAILTRLLIQPTFIRSF
                     TQQADPYDLRKLQEKIMLASVRDESWLVVGNDENECSFRLEDNQLLIKNVLSISVFKE
                     KQFLIRDFIQKKMIAHGVFAYMRAYSEFIYHNTKQISQRLLFEKKDEIEHLPKMKQQN
                     GEVVVDCNQFPGYDLFYQGLCFTSCWEMYYSRYYHQIIPKPIFLDIQQVEKVKELENE
                     VICIQLYRDPFNWQKPNNQMFQSYFRDQLGFDHLAWDNGVGLLKPPFVEYIYTDHTIQ
                     SVQYQNAQMQPVPKKNASFFVTKSYDIQQGDYKERRVRGTLNAQAYFPWVDENRSRMM
                     CYKVIDPTVALDNGIEAYCYYIREYLEVEVTDEKYQEYLLSLRIYVPSESLSELPLKE
                     IKHRLSDVTFKRIKKKRRSIQFDVKKGEQHLRVQFLDYRELEQLITLQKI"
     gene            18795..>18990
                     /locus_tag="IV334_001519"
     CDS             18795..>18990
                     /locus_tag="IV334_001519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8943592.1"
                     /translation="MDKQLYYRIMVPASLLSVCLFFVPNFPIRSVIGFLVMFGGMGLY
                     YYLLHKERKPASVYMKKTRSE"
BASE COUNT         6200 a         3385 c         4090 g         5315 t
ORIGIN      
        1 ttgcttctgc ttccgccgtt tatacgtgtt tagagcgtga gacaaaagtg ttctttactt
       61 ttgtcccacg ctccttattt ttttattttg tttggatcaa ttcttttaat tcctctattg
      121 aaatagattc atcaaagatt tctttaccca agatgacagt tggaacaaat cgaatatgtg
      181 cttgctcagc ttcgttgatg acagcttggc tcagctgttc gtctttttgt tctgtcagct
      241 ttagtttatc tactgcaaaa tccgctactt cttggagaga aagatgtttc cactcgtctt
      301 gagtatcgaa tatttgtttg atctctttga tcgctttttg gccatcgctg attgtgagat
      361 accggtgcat cacgttccct cgctgtaagc tttctttttc tttatcgaac aatttgatca
      421 ctcgctgcaa ttgcccgctt tggactgctt cttctaatag ctcataagat tcttcaaacc
      481 attgtcggca atacggacaa gctaagttga tgaattcgac cattttttta ggagcattcg
      541 atgaaccata aaggattcct ttttgtgtat ttgttttcgt tgcatcaatc actgatatat
      601 ccatgttaaa acacctcttt cgcttatagt ttatacatgt ctgacaaaaa aaggcaacga
      661 tttagactgt ttgtctaaaa attagatgac caattcaaat agatacatgt aaaatatttt
      721 tataaataaa agaaaaaaat ggcatgtttt acgttataat ggaacagata ataagagaga
      781 atatgaacaa ggatgatgaa tcatgcgaaa aatgaaaaca atggatggca atacggcagc
      841 cgcatatatt tcttatgcat ttaccgagat tgctgcaata tatccaataa cacccagttc
      901 gaccatggct gaattagtag atcaatggtc agcagcagga aaagaaaacg tattcggtca
      961 accagtaaaa gtagtagaaa tgcaatccga agcaggagca gctggtgtgg tccatggatc
     1021 attgaaaact ggtgcactga caactacata taccgcttca caagggctgc ttttgatgat
     1081 accgaatatg tacaagattg ctggggaact actgccaagt gttttccacg ttgctagtcg
     1141 ggcagtgacg acaaatgcgt tgaatatttt cggagatcac ggagacgtga tggctgcaag
     1201 acaaacagga tttgctatgt tagctgaaag cagtgtacaa gaagtgatgg atttagcacc
     1261 tgttgcccat cttgcttcta ttgaaggaga agtccctttt ttgaatttct ttgatggatt
     1321 ccgtacgagt cacgagattc aaaaaatcga agttttggaa tatgaagaat tagctcctct
     1381 gatcaatcag gagaaattag cttcatttcg taaacgaggg atgaatccca atcatccgac
     1441 tgttagtggt acgaaccaga atccagacat ccattttcaa caacgggaaa cgatcaaccc
     1501 ctattatgat aaattacctg aaatcgttca aaaatatatg aaagagatca accgattacg
     1561 aggaacttct tatgacttgg tgacttatta cggagcagac gatgcggaag aagtcattgt
     1621 ttctatggga tcagttgcac agacgattga acaaacgatt gattacttga atgcaaacgg
     1681 acggaaaaca ggttttttga atatccatct ttatcgtcct tttccagtgg aaacattcgt
     1741 gcaaaagttg cctcatacag taaaaacaat tgctgttctt gatcgaagta aagaaccagg
     1801 ggcaggtgga gaacctctct tattagacgt tcaaagtgcg ttatatgatt ctgaacttcg
     1861 cccggctgtt attggtggtc gctacggatt ggggtctaaa gatgtgactc ccgaccaaat
     1921 ctcagcagtt tttgatgaat tgaaaaaaga cccaagcata cgcaaaaaac ggtttaccat
     1981 agggatcgtg gatgatgtga cttatcaatc tttggaacca aaggaaagtc tggatttgac
     2041 agaaccgcaa acattccagg ccaagttttg gggatttgga tctgacggaa cagttggagc
     2101 aaataaatca gctatcaaga ttattggaga tcacaccgat aaatatgcgc aaggctattt
     2161 ttattatgat tcgaaaaaat ctggcggtct gactgtctct catcttcgtt ttggagatac
     2221 gccgatacgt tcagcgtatc ttgtcgagca tgcggacctt gttgcctgtc acacaccagc
     2281 ttacctgcat agctatgact tggtcaaagg attgaagcca ggtggcattt ttttattgaa
     2341 tactctttgg agcgacgaac aattagaaac gcatctgccc ttaaaattaa aacgttactt
     2401 ggctgaaaat aacattagat tctatacgat caatgccatg agattggccc aagaagtcgg
     2461 tttgggacgc cggatcaata cggcaatgga aacggctttt tttaaattag ctgacatcat
     2521 tccttttgat gaggtgcttc cgctgctaaa agaagaagcc ctgaaaagct atggacataa
     2581 atcaatggct atcgtagaaa aaaacataca agcaatcgat cggacgaccg aactgcttca
     2641 tcaagtcgcg atcccagacc attggagaac gttggagatg cctgaaaaaa tcaataaaag
     2701 gaacactggt tatatacaag aaatacttga accagtgaat gcacaggaag gaaatcaact
     2761 ctctgttggt acacttgtag caaatggaat gacaactggc gaaatgcctt taggaacggc
     2821 ttctatggaa aagcggggcg tcgctttaga agtacctgag tggattagtg atcgctgtac
     2881 gatgtgtaat gaatgtgcct ttgtttgtcc tcatgctgcc atccgtccat ttttagcaga
     2941 tgaagaggaa atggaagaag cgccagaagg atttatcgtg agagaaatgc gaggcgcaga
     3001 cggcgtaaaa taccgtatcc aagtttcagt ggaagactgt acaggttgtg gtctgtgtgt
     3061 agaggcttgt cccgcaaaag aaaaagcttt agtgatgaag ccttatgaag aacagaaaga
     3121 acaagctgtg aattgggctt ttgcaatgac attaagacaa aaagcaaatc cagtcaaagg
     3181 aaagaatact gtcttgtcta cacaattcaa tcaaccgctt cttgaattct cgggcgcttg
     3241 ctcaggctgt ggcgaaacac cttatgtaaa attattgaca caaatgttcg gtgatcgaat
     3301 gatgatcgcg aatgcgacag gctgttcttc tatttggggc ggtgcttctc ctgcttctcc
     3361 ttatacgacc aatgaatgtg gtcaagggcc tgcatggtca aattctttat tggaagataa
     3421 tgctgaattt ggttatggta tgcttctagc ggcacagact cgtcgcgagg ctttggcaag
     3481 ttcaatcaaa gaagtattgc caaaagtatc agaagaacta cgagaattga tggaagactg
     3541 gttagtacac atgtttgaga gtgaaggaac gcagcaaaga gcagcaaaac tgcaagctgc
     3601 gatcgaaagc gaacgatcaa ttttccctga actggaggat atatacaaga gaaaggatct
     3661 attcgtaaaa aacagccaat ggatgattgg tggagacgga tgggcttacg acattggttt
     3721 tggcgggatc gatcatgtac ttgccagcgg agcagacgtg aatatccttg ttttggataa
     3781 cgaagtctat tccaatactg gcggtcaaac ctcaaaagca acaccagctt cggcaatcgc
     3841 taaattttcg gctagtggaa agtatgcttc aaagaaagat ctaggaatga tggcgatgac
     3901 atatggaaat gtttatgtgg cgcaaattgc ttcaggtgcc aatcaaatgc aaacgatcaa
     3961 ggcatttgaa gaagcagaaa agtatcctgg tccttctctg attattgcct atacgccttg
     4021 tatcacacat ggcttgcttg gcggaatgaa agaatcgctt aaagaagcaa aagaagctgt
     4081 ttcttctggt tattggtcat tatatcgata taaccctaca atggtcgaaa aaaacaagaa
     4141 cccaatgact ttggactaca aaaaacctga tttctctgct atgcctgatt tcatgaggaa
     4201 acaagttcgc ttttcctcgc tagataacgc aaatccaaat cttgcaaaag ctttatttga
     4261 aaagactgtc cacgacgcta aaacacgttt ttacaactat gctgcactga cgggagaatt
     4321 agaaaaaatc aagaaacgac ttgaaccaga ggaatctatc gatgtacccg ttaagactag
     4381 aaaagaaaaa acgacggacc ttgaagcaga agaaagaaga gctgctagaa gagctgccag
     4441 agcagaacga cgtaaaaaaa tggaacaagc tacaaaagaa aaaaaggaaa aataacagaa
     4501 aaaatataat gaataattct ctgaagttta aaaatattta ttgcagagaa aaataaaacg
     4561 catacttgca attttcttga aaattatgcg tttttctcaa gaaaaatacg agcatatcag
     4621 cctctaaaat taaaacatat gatatactag agaggaaatc tcaaaagaga agagaggctt
     4681 tttatgtcat ttcaaatcgg ggaacttttt aatttggact attggcagca aatgttttcc
     4741 ggtgacttgt tctctctgaa ttttttggtg aacatacttg atattctggt tgtctggtat
     4801 cttgtataca aactgatcca actgctgcga ggcacaaaag cgattcagct tctgaaaggt
     4861 gtagctattt ttattgttat ccgaatcgta gcggaaatca ttggtctgca caccttgtct
     4921 tggctgatga accaagtcat tacgtacggg gtgatagctg ctatcgtgat ttttcagccg
     4981 gaaatccgga gaggactgga acatcttgga cgcagttcgc ttttcaaaca atcaaaaagc
     5041 gaacagcagg aagatgagaa gatgatcttg tcttttgaca aagccatcca atatatgtcc
     5101 aagcgaaaaa tcggtgcgtt gatcactatc gaacgtcata ctggattaga tgagtatatt
     5161 gagacaggga ttgctttaga tgcagatatc actggggagc tgctgatcaa tatctttatt
     5221 cctaatacac cgctgcatga cggtgcagtt attgtaaaag aaggaaagat cgcagtagca
     5281 agcgcctatc ttcctctatc agaaagtatg ttgatcccta aagaattcgg gactcgacac
     5341 cgggcagctg tcggtataag cgaagtcagt gacgcgatta ccatcgttgt ctctgaagaa
     5401 accggggacg tcagcatcac gttagataat gaactgatgg ctggcttatc ccagcaagaa
     5461 tatttggcaa tcttgcgtag agaactaatt gctgaagaga aaacaaaaaa caagaaaaac
     5521 gtgattcaat ctttcctaga cggtatgact aagggagggg atcgcaaatg atctcaaaag
     5581 agaaacggac aagcattctt tacagtttta ttgcattgct gttcagtatt gcgctgtact
     5641 tcaatgcaaa tggccagtcc gttccaaata cgctttctgg aaatgaagcg tataatcaaa
     5701 cagttacggg ggtgccgatc aagatttctt atgattcaaa gcgctactat atccatggat
     5761 atgaaaatgc agtgactgtc aaactttcca gtgcaaaccg agttcagttg aatgcagagg
     5821 caaacgaaga tacacggatg ttccgcgtca cagcagatat cactaagctg ggcgaaggaa
     5881 cacatgaagt tccgctaaaa attcaaaatt tgagcagtgc tgtcacagca aagatcgaac
     5941 catcgactat tacagtaacg gttgagaaaa aggtaataaa agatttcgat gtggaaacgt
     6001 tgattccttc aacgttcaca ccatctggat atgaacttga tgatacttcc gttagtccta
     6061 aaaaagtctc tatcactaca ggtgacaaaa cattggcaga aatcaaacga gtagtagctg
     6121 atgtggacgc ttcgatggtc acagatgatg ggattaatag tgaggtccca atacaggcgc
     6181 tgaatgcagc aggggaagta ttaagtatcg tctctgatcc agtgcaagta acagtgagag
     6241 ctgattcgat caagccaagc aagtcggtac gactttatgg gatacagcaa ggaacaccag
     6301 ctgccggggt agaaagctat gatttcagtt tttcaaaact ggaagcagaa gtaagcggct
     6361 ctagcgattt gcttgcatcg atcggtgaca gtattgctgt accgatcaat gtctcaggta
     6421 tcaatcatcg aacaactcgt aaaattgaaa taccagtaga agaggggtta agtatcaagc
     6481 ccgaatcagt tagtgtggag attacacccg tgttgcaaac gtcaagcagt agctctgctt
     6541 cgacgagcag tcggacacaa gaaaatacaa gtacgagtag aagtatttca tctgctgatt
     6601 caggaacgga accatcgaca acaaccattt cttccagttc ggaatctggg acaagcagct
     6661 cgaccacaag tacagaagaa acatcagaaa caagcgaatc aacacaaatg agcagaacaa
     6721 attcctcaga aaattaagtt aaaggagtaa ttttacaact atgggtaaat attttggaac
     6781 agacggcgta cgcggtgaag ccaacaagga acttacgcca gaacttgcat ttaagctggg
     6841 acggtgcgga ggttatgtcc taagccagca tgaagaagaa ggaaaacgcc cacgcgtgtt
     6901 agtagggcgg gatacaagaa tctcaggtca aatgttagaa gaagcgctga tttcaggact
     6961 tttatcggtg ggtatcgaag tgttccagct aggtgtgatc tctacaccag gtgttgctta
     7021 tctgacacgt ttgcaaaaag caagcgcagg ggtcatgatt tctgcttctc ataatccagc
     7081 acaagataat ggaatcaaat ttttcggtgc agatggtttt aaactagtag atgaccaaga
     7141 gctagaaatt gaagcattat tagatgctga agtggatgaa ttgcctcgtc catcagctga
     7201 aggattggga actgtagaag aatttccaga aggcttattg aaatattctc aattccttgt
     7261 acaaacgatc aacggtgacc tttccggttt gaccatttgt gtcgatgcag caaacggagc
     7321 aacttcaaca agtgttaatc gtttgtttgc cgatcttgaa acagattttt atacaatggg
     7381 cacaaatcca gacgggttaa atatcaatga cggcgtaggc tctacccatc cagaacgttt
     7441 agctgagatg gtcgttgaaa aaggagcaga tgctggacta gctttcgatg gcgatggcga
     7501 tcgtattatt gcggtggatg aacttggaaa cattgtcgac ggtgataaaa tcatgtttat
     7561 ctgtgctaaa tacttggcag aaaaaaatcg attgaaaaaa gatacgatcg ttacgactgt
     7621 catgagtaac ttaggattcc acaaagcggt ggaaggaatc ggtttgaaag atgtcgttac
     7681 acaagttggt gatcgttacg ttgtagaaga aatgcgcaaa aacgattata actttggcgg
     7741 cgagcagtct gggcacatga ttttcttaga ttttaacaca actggagatg ggatgctttc
     7801 aggaattcag ttactgaata tcatgaaaca gacaggtaag aaattatcag agcttgcagc
     7861 agaggtaaca atctatccgc aaaaattagt gaatatccgc gtaagtgata aaaacggtgc
     7921 gatggatgtt ccggcaatca aaaaagtgat tgaagaggca gaagctgaaa tgggatcaga
     7981 aggaagaatc ctagttcgtc cttccggtac agaaccatta cttcgagtga tggcagaagc
     8041 gcctactgat gagaaagtta attactatgt cgataaaatc gcggcagtag tcaaagaaga
     8101 aatcggactg gattaagaga tattttcata aaaaataaaa gaacaaggac cagtgtcaga
     8161 tgtctgacac tggtccttgt tcttttatta ttctactgtc actgatttcg ctaaattacg
     8221 tggtttgtca acgtcgaatc cccgtaatag cgttgcgtaa tatgcaagta attgagtcgg
     8281 tacgactgtg gttagagtag ttagataagg gtggacagtt ggtagcacca gctggtcact
     8341 ttccttttct aatccctctg agacaataac tagtgtttta gcgccacggc tttcgacttc
     8401 tttcaggttt cctcgagtat gcgcaccagt cacttcttca ctgatgattc cgatcactgg
     8461 tgttccttct tcgatcaaag caatcgttcc atgtttcaat tcgccagcgg caaaaccttc
     8521 tgcctggata taactgattt ctttcaattt caaagcagct tcaagagaaa cgttgtagtc
     8581 agcaccacgt ccaatgtaaa aggcatttct tgtatcgctg agatatactt ctgccaactc
     8641 ttctaaataa tctttttggg cgatgaccga ttccatcgca ttagcaacta aactcaattc
     8701 atgagtgata tcgaatacaa gagaagtttc aaattctttt ttatcaccga tagctttggc
     8761 taataaagtt agtacagcta tttgtgcagt ataagctttc gttgatgcta ctgcgatttc
     8821 gggtccagca tgcaatagca gtgtaaagga tgcttcccgc gaaagagtag aaccagcaac
     8881 atttgtaatc gtaagtgaag ggtagccgag acggttgatt tttaccagca cttggcggct
     8941 gtctgctgtt tcgccacttt gactcaagaa aataaagaat ggtttttttg aaagaagagg
     9001 tgtgttatag ccaaattcac tggctaaatg aacctcaaca ggaatctgtg tcaatttttc
     9061 caaagtctgt ttacctgcta accccgcatg gtaactagtt ccgcaagcaa cgatatagat
     9121 tcgatcgcta tcagacaatg tatcgaccag cttttgatca agagagacac gattgtcttc
     9181 tgttacgtat ttttgagcga tacgacgcat gataataggc tgttcgtcga tttctttcag
     9241 catataaaaa ggataggtac ctttctcgat atctgaagca tctacttgtg cttcgaatga
     9301 agaacgttct acgatgtcac ctgcaaaatt ttgaataata actttttctg atgtgacggt
     9361 aaccatctct tcatcttcaa tctcaacgaa ttccttcgta tgagcaagca tagccattgc
     9421 atcgctggca atcacattga atccatcgcc gagtccgatt aatagtggac ttttattttt
     9481 agcgacgtaa ataacatcag gtgctgtccg atcaatcaaa gcgaaagcgt aagaaccttt
     9541 gatgacccgc aaagctttta aaaacgcctc ttgcgttgta gtatccttcg cgaaagtttc
     9601 aattagatga gcgataatct cagtatcggt ctcaccaata aagtggtcat ttgctaaata
     9661 agcaatttta agttcttcaa agttttcgat tacgccgttg tgaacgagca cgaagcgtcc
     9721 actttgggaa gtatgtggat gggcgttttc ttcagaaggt tgcccgtgag ttgcccatcg
     9781 tgtatgacca atacctatat ttcctgtagt atcaatattt attttgtttt tcaaattttg
     9841 aattctgcct tgtgctttga ccagataatc agttgtgcca tctgagacaa agataccagc
     9901 tgaatcataa ccacggtatt ctaatttttc tagaccgtca atcaatcctg gtgttacatt
     9961 ttttaatcca atcattccaa caattccgca cataaaaaac atcctacttt ctataatgaa
    10021 ttcgtatgaa tgcttccagc tgcaggaaaa tccttttgtt ttatctttgc agacattttt
    10081 tgtggatttt ctgtagagtg cagtccctag ggccatccgc cgaatctttc gataaaccct
    10141 ctcctcgtca tctgtttaca cagacctggc gctatcctaa aaggtataac tatcctcctt
    10201 tttgtttttt atctggaaac atttaaaatc ataacctttt gcgaatgttc ttgtcaacaa
    10261 aaaaagaata aaaaataaat tggtatatat caaattcttt gtcaaatgtc ctttttatgt
    10321 caaaaaataa tgggttttta tctatcatct ttttcaggag ttcggtacaa tagaaagtaa
    10381 tcggatagag gaggagtaaa aatggattca aaaattgaga tcatgacact gggaatgtta
    10441 aaaaaacaat tgtccaaatt cgaagcatca gctggtgtat cagatgatac gaaaatattt
    10501 ttagatactg gctgggatag catccaagaa atcgcacctg atgccttgga agttgctcaa
    10561 gctcgtgagt ttacggtaga agatgaatta acaaaagagt cgttttccgg gtatgcgcgg
    10621 gaagaaaaag cagagcggtt cgacacttct gagcagtcag aaactgtgat cgtcataaaa
    10681 aacttgtatt agaaaggaga aagaatgaat aaaagacgtt gggcagcagt tctgattgct
    10741 gcaggattat ttgtggtttc gttagtttca gctagtttga ctaagcctca ggaaaacgat
    10801 caaattgagg gattgaacag ttggttatat ggggatgaag aactagctcc aaatgtgttg
    10861 gaagagggag acagtacgca aaaaatcgta aaattatcag tagatggaac gattgctgcg
    10921 ggagggtctt caggtttatt tgcttctgaa ggttacaatc ataaaaattt tatggaacaa
    10981 ctgaaggcga ttgaagaaga tccaacagtc aaaggtatct ttttagaagt aaattctcct
    11041 ggtggcggtg tctacgaaag tgcggaaatc gctaagaaat tggatacgat tcgtaaagaa
    11101 catgatatcc caatgtatgt cagcatgaaa aacatggcag ctagcggtgg ctactatatt
    11161 tctgctcaag cagataaaat ctttgcaaca gaagaaacag tgaccggctc aattggtgtg
    11221 atcatgtcag gattgaatta ttcaggactt ctagaaaaac tgggtgtgga agatacaaca
    11281 gttaaaagcg gagcgttgaa agatatgggt tcaggaacgc gaccagagac aaaagaagaa
    11341 gaagcggtgc ttcaagcata tatcgacagc gcatatagtc gttttgtagc aatcgtcagc
    11401 gagggacgga ataaatctga agaagaagtc aaaaaaattg ctgatggaag gatttatgat
    11461 ggtgtccaag caaaagaagt aggtttagtc gatgaattgg gatttcctga agatgcttta
    11521 aaagctatac gaaaggaaca acagcttgaa gatgcggaac tagtagaata ttcttccacg
    11581 ccatctgggt ttgctagtac atggttagga agtaaactag cagaattcca gggattgaag
    11641 ccttctgaga ctagtcagat cttatccgtt cttgagaatc tagggacagc ggaatcacca
    11701 aaagcaatgt attattacgg aggtgagtaa gcatgtcaga gctttcaaat gaaaataagg
    11761 aacaagaaaa agataagcaa cagcagcctg ctgtaggacc tgactccaac ttttcccaaa
    11821 aggtactgag ctcttttgaa agagagcctt tgacgcccga agaaatcaaa aagaaacaac
    11881 gtaaatggaa aaagtatacg gacacaagaa atgaaccgcc aaaagcaaat gattttccta
    11941 agtatttcta tgcaggtttt tttatccgaa tgtttgcctt cttggtcgat cttttgtgta
    12001 tcggagctat tacgcgttta acacttggta ttgccaaaaa tctaggttgg cttaattttt
    12061 cagattctta tctgagtctc tacggattca cagccttact tatttatcta gcgtatttta
    12121 ttttattgac gaaattcaat catggccaaa cgattgggaa aatgatcttt ggtattcgag
    12181 tcatcagctt tgatgaagaa gaacttagct ggtcaaccgt tttagttaga gaaggtgctt
    12241 gccggttcat tctaaaattt cctttgctga tgttagggta tcttccagca gtctttaatc
    12301 agaaaaagca acatctgggc gattttttca ctaatacaag tgttgttacg atcaatctga
    12361 tcaaagcgtt caaccaggaa gtcaacagct gatgagcaat aaaaagcagt aacagttgaa
    12421 agcaccttct aaaagtcaga tctaaaatct agcttttaga gggtgcttca gttattttta
    12481 attgtcatgc tttttttgtt ttgctactat tgaacatgag ctgaatatga gcaagaggag
    12541 gacactgcgt gaagccgatt gattttccag aaaaatatga gaatttaatg cgagtggcac
    12601 aacaagcact agcaaaccag caatatcagc aggcaaaaga attatttcaa cgagcttacg
    12661 agttgaaaga aagctttgag gctaatagcc tgcttgtttt ttgtctttac gagttggacg
    12721 agaaaaaaga agcattgaag caagcactct tgcatgaaaa acagtatctt caaaatgagg
    12781 agtttgctga attttatttt gacttgttaa tcagtacaca agatttttta tatgctagaa
    12841 aattgatagc aagtacagat ttttatgaag ctttcgagca gcgggtcatt gaaaaaatcc
    12901 aatttgcaga agaattatcc ggacaaatgg agaggcagaa gatcaaggca ctccacaata
    12961 aatcggagga acttccttcg ttggaacctg cacgtcagct tgcgtctatt gatgagatcg
    13021 aacagcttcc ttatcatgag tttatccaaa cagcgagtaa acttattgta tcgccagagg
    13081 tccatatttt agcaagggca aaattactgg agacacttag gcaattgaac gaatgccatc
    13141 ctgttttcta tttgacgatt gaagaaaagt tagtcaaagt cataccaaaa gatctgccaa
    13201 agccgcagca gcaggcaagt taccagcaat tatgtgtatt ttcagatcgt tacgggaatg
    13261 aagatgcgct gcttagttcc gtgttaaaag aagaattcac gttgcaatct gcaattgttt
    13321 atcctgtcta tgatacatac atcgaagacc cgaagcgctg gtttcaactg actgtagagg
    13381 cgtgtacagg aaaaacgatg tatgaaggta cagaagatga aaaacaagat tttttaaaaa
    13441 aaagagaaaa aattttgcag caaatgatct tcttccatta gattttatca gtgaaaaaga
    13501 taaaaatgtt atgaaaaaca aggattctat ccattcaata tgtttacaat ttgaaagggt
    13561 tagtgtacta tttaaaggta tgcatgaaac gtttttgatg tataaaatga atttctaaac
    13621 tcggagggaa ttaaatgact gcaacatttg aaaaaaaagg cacgaatgat ggtgttttaa
    13681 aatttacgat tgcgcaagac gaaatccaaa aaggattaac aactgcgttt aataaagtga
    13741 aaaaaagctt gaacgtacct ggattccgta aagggaaagt ttctcgtcaa gtattcaacc
    13801 gtatgtacgg tgaagaagct ttatatgaag atgcattgaa tgctgtatta ccagaagctt
    13861 atgaagctgc tgtcaaagaa gctggcatcg atcctgtttc tcaaccaaaa atcgatgttg
    13921 aaagcatgga aaaaggtcaa gactgggtaa tcactgctga agtaacagtg aaaccagaag
    13981 taaaattagg acaatataaa gatctagttg ttgaaaaaca agaccgtgaa gtaacagatg
    14041 ctgacgtgga tgcacgtatc aaacgtgaac aagaagcaca agctgaatta gttatcaaag
    14101 aagatgctgc agctgaaaat ggcgatacag tagtaatcga cttcgaaggc tttgtagatg
    14161 gcgaagcgtt tgatggcgga aaaggcgaaa attactcact tgaattaggt tcaggctcat
    14221 tcattccagg ttttgaagat caattagttg gtcataaatc aggtgaaagt ctagatgtaa
    14281 atgtaacttt cccagaagat tatcaagcag aagatcttgc tggaaaagaa gcagtcttca
    14341 aagtgacaat ccatgaagta aaagcaaaag aagtgcctga attagatgac gaattcgcaa
    14401 aagatgttga tgacgaagta tcaacattag acgaattgaa agaaaaatat cgcaaagaat
    14461 tagtagaaac aaaagaaaaa gcagcggacg atgcaaaaga tgaagcagcg atccgtatgg
    14521 ctgttgaaaa cgcagaaatc gttgaattgc cacacgtgat ggttcatgat gaagtacatc
    14581 gttcaatgga cgaattcttg aacaacatgc aacgtcaagg tatctctcct gaaatgtact
    14641 atcaattaac tggttctaca gaagaagact tgcataaaca attcgaaggt gaagcagaaa
    14701 tgcgcacaag aacaaatctt gtgatcgaag caattgcagc tgctgaaaat cttcaagcaa
    14761 cagaagaaga aatcaatgca gaaatccaag aattatcaga aacatacaac atgccaatcg
    14821 aacaaatcaa acgcgtgttg acagaagata tgctggaaca tgatgtgaca atgaaaaaag
    14881 caatcgaatt gatcacaggt acagcgacag aagcgaaaga atagaaatca agtaataaat
    14941 catttctgaa gaagctactt ctagaaaatt gtttattcga aataagggac tgtgacacga
    15001 ttttgtcaca gttccttatt ttttgttaag agtaaaaaat gcaaaggtat aggtcagttg
    15061 atcctttgtt aaacactttt tctttttact aaactatgtt atgatagtaa aagactgtaa
    15121 agtctttgat agtttcttgc atactttttg caagttaagg ggtgaagacc atgtatgaca
    15181 atccaagtag caatgagacc gtacgctgtt ctttttgcgg gaaaacccaa gaagaagtga
    15241 aaaagatcgt agctggtcca ggggtctaca tctgtaatga atgtatcgac ctatgtaaag
    15301 aaatcattga tgaagaattt tatgaagaag ctgtacgcga attcactgaa gtccctaaac
    15361 cactagaaat tcttgaagta tttaataact acgtgattgg acaagatcgc gcaaaacgct
    15421 cgttagctgt tgccgtatac aatcactata aacgtgtcaa tcagcaagca caagaaaaca
    15481 cagatgatgt tgaactgcaa aaaagcaata tctgcttgat cggacctaca gggtctggta
    15541 aaacattttt agcacaaaca ttagcaaaaa cattgaatgt accttttgca atcgcagatg
    15601 cgacaagttt gacagaagct ggttatgtgg gggaagatgt agaaaacatt ttgcttaaat
    15661 tgcttcaatc tgctgattac aatgtggaac ttgcagaaaa agggatcatc tatattgatg
    15721 agatcgataa aattgcgcgt aaaagcgaaa atgtatctat tactcgtgac gtttccggcg
    15781 aaggtgtcca acaagccttg ctgaaaattc tagaaggaac tgttgcaagt gtgccacctc
    15841 agggcggacg taaacatcca catcaagaat ttattcagat tgatacgacg aacatattgt
    15901 tcatcgtagg tggtgcattt gatggcatcg aaacgattgt gaagaatcgt atgggcgaaa
    15961 agacgattgg atttggtaca caaaaccaaa aactttctga agacgaaagt gtcatgcagc
    16021 atatcattcc tgaagattta ctaaaattcg gattgatccc tgagtttatc ggacgattac
    16081 ctgttatggc agctttagag aaattgacaa cagatgatct ggtacggatc ttgactgagc
    16141 cgaaaaatgc gctagtcaaa caatatcaaa aacttctttc tctagataat accgaacttg
    16201 aattcgaacc tgaagcgttg agagccattg cgaaaaaagc aatcgaacgc aataccggtg
    16261 ctcgaggact tcgttccatc attgaagaaa tcatgatgga tgtgatgttt gatatccctt
    16321 caaatgaaaa catcgaaaaa gtgattatca caaaagaagc agctgaattg tctggtgagc
    16381 caacagtaat ctataaagat tcagagaaaa aagcgggttg atgaaaagag gctgccgtaa
    16441 aagtcatttt ggcttttgcg acagccgctt gctttattca tcaccttgtt aaggagagag
    16501 accatgaaag tccatcaagc tgaaattatc ataagtgcag tggcaccaaa acaatatcca
    16561 gatacacaat tgccagaaat agctctggca ggacgatcaa atgtagggaa atcttctttt
    16621 atcaacacat tgattgatcg aaagaattta gcccgtactt ccggtaagcc aggaaagacc
    16681 cagacattga atttttattt gatcgagaac gcattacatt ttgtcgatgt tccgggatat
    16741 gggtatgcaa aagtatccaa aactgagcga gcaaaatggg gaaagatgat cgagacatat
    16801 ttaacgacta gagaccagct acgtgcggtc gtttctttag tagatttgcg tcataagcct
    16861 tctcaagaag atatccagat gtatcaattt ttgaagtatt atgatatccc tgtaattgtt
    16921 gttgccacta aggcagataa gatccctcgt ggtaaatgga acaagcatga gagcgaaatc
    16981 aaaaaagcgt tggattttga tcgcaatgac acgtttattc tcttttcttc cgttacaaaa
    17041 cagggaaaag aagaagcgtg ggcagcaatc gaacaagcaa ttgaatcata aaaagaataa
    17101 aaaagactta ccagtcaaac tctttcataa aagtttggct ggtaagtctt tttttgagct
    17161 taacttactt ttgtttcttg tcttttttct tttcattttg tggctgtggt tttagaaatc
    17221 gatcgatctc ttcttttggt gcaactgttt tttccggatc gacagcaaat gattcgaaaa
    17281 gtttatctaa attatctgta cgttcaaatt tcaaccccat tgtaaacacc tctttgtttc
    17341 tttttactta gtctatcatg taatcgtcca aattggtttc taagacataa agttttagtt
    17401 tttctttatc attggttata attaaattat aaaaagattt gaaagaggtg agaaaatgat
    17461 tggcgaagat aaattattga tgactatcca gctcacagac ttaaatgaag aagaaaaaaa
    17521 acaggaaata agcgattggg ctattttgac acgtctgttg atccagccaa catttatccg
    17581 ctctttcaca caacaagcgg atccgtatga tcttcgaaag cttcaagaaa aaataatgct
    17641 agcatcggta agagacgaaa gttggttagt tgtaggaaat gacgagaacg aatgtagttt
    17701 tcgcttggaa gataatcaat tattgataaa aaatgtattg tctatctctg ttttcaaaga
    17761 aaagcaattt ttgattcgag acttcattca gaaaaaaatg attgctcatg gggtgttcgc
    17821 ctatatgcga gcttatagcg agtttatcta tcacaataca aaacagatct ctcaacggct
    17881 attgtttgag aaaaaggatg aaatcgaaca tttacctaaa atgaaacaac aaaacgggga
    17941 agtagtagtc gattgcaacc aatttccagg atacgatctt ttttaccaag gtttatgttt
    18001 cacttcgtgt tgggaaatgt attatagtcg ctattatcat caaatcatcc ctaaaccgat
    18061 ttttctcgac atccaacaag tagaaaaagt gaaggaatta gaaaacgaag tcatatgtat
    18121 ccagctttat cgagacccct ttaactggca aaagccaaac aatcaaatgt ttcaatccta
    18181 ttttagggac cagcttgggt ttgatcatct tgcttgggac aatggtgtcg gtttattgaa
    18241 accgccattc gttgaataca tttacactga ccatacgatc caatctgtcc agtatcagaa
    18301 tgcacaaatg caacctgttc ctaaaaagaa tgcttcattt tttgtaacaa aaagttatga
    18361 tatccaacaa ggagattata aagaaaggcg tgttcgcgga acactaaatg ctcaggcgta
    18421 ttttccttgg gttgacgaga atcgatcacg catgatgtgc tataaagtta tcgatccgac
    18481 tgttgctttg gataatggaa tcgaagctta ttgttattat attagggaat atctagaagt
    18541 agaggtaaca gatgaaaaat atcaagagta tcttttatcc ttaagaatct atgttccttc
    18601 agaatccctg tcagaattac cgttaaagga aatcaaacat agactctctg atgttacctt
    18661 caaacgaata aagaaaaagc ggcgaagtat ccaattcgat gtcaagaaag gtgagcagca
    18721 tctgcgtgtg caatttttag attaccgaga attagaacaa ttgattactt tacaaaaaat
    18781 atgaggtgaa gaaaatggat aaacaactct attatcgaat aatggttccg gcatcattat
    18841 tatccgtctg tctctttttt gttcctaatt ttcctattcg atcagtcatc ggttttttgg
    18901 tcatgtttgg cgggatggga ctttattatt atcttcttca taaggaaaga aaacctgctt
    18961 ctgtttatat gaaaaaaaca agaagcgaaa
//
LOCUS       DACDKM010000047        18740 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20834_2016
            SAMN12640370-rid9891663.denovo.047, whole genome shotgun sequence.
ACCESSION   DACDKM010000047 DACDKM010000000
VERSION     DACDKM010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640370
            Sequence Read Archive: SRR10040891
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 18740)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18740)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742835.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742835.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:04:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,012
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 29 of 75
            Pseudo Genes (incomplete)         :: 33 of 75
            Pseudo Genes (internal stop)      :: 19 of 75
            Pseudo Genes (multiple problems)  :: 5 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18740
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640370-rid9891663.denovo.047"
                     /isolate="E20834_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            256..531
                     /locus_tag="IV334_001520"
     CDS             256..531
                     /locus_tag="IV334_001520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8943593.1"
                     /translation="MRESFEQQKKLLHDRYGALSMDDRRQILCKLRKRNILMYRQLER
                     LKHDLLRLESKRVQCELEGNQTQVEVVETKILKKKEQFLKMLTQNKK"
     gene            544..2616
                     /locus_tag="IV334_001521"
     CDS             544..2616
                     /locus_tag="IV334_001521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton antiporter"
                     /protein_id="HAP8943594.1"
                     /translation="MEFAELVIVFAVTITLSNIASRILPMIPAPLIQIFLGVVLGLTE
                     WGQSIDFEPELFLVMIIAPLLFREGEKADISSILKNFGTILFLAFGGVILTLVGVGAT
                     LSFLLPSVPLAACFAFGAALGPTDAVAVSSLSGRVNIPKKAMHILEGEGLLNDASGVT
                     AFQFALGALITGSFSAVNAGMSLVVSSIGGALIGFLLVWFKQKIIHLIEKASAQDVTG
                     YLLIELLLPFLAYVLAEAFDVSGIIAAVAAGILQASGFRKISVFDAELSSLSHSTWTT
                     IAFTLNALVFIFLGIELTQVFSPVWGDGLYPNGLLLAIIVLISVMLFVIRFISISLFY
                     VFKDGSKKFKKQLNEILILTFGGVKGTVSLATIFILPFSINNMMFYQRSLLLFLTAGV
                     ILVTLVIGIIVLPMLTETEEAKSTDLNALMILEEVVEVLRKEIKEIDRNTKEFLATEA
                     VIENYQERIRDLYLEDLTDDEKQEVQEIQALILSIERDGLDESYRSGKLSSNGYRFYS
                     RFLSRFEHSITSQILSFIGFWFIVVRRLMRIVLHPKMFWQRRYADRQTFISDEDIQEI
                     RETYQKNTQLIIESLDNLTDIYDETMVQFFIQQRKLEGIKMNSGNLISSWMIQQDSLF
                     TKKMLRGYYLERKIIDEYEVAEKITTFSANSYRRNINLLESYTMNKPSDNFSFRFAFR
                     AKSKKRVQ"
     gene            2781..4667
                     /locus_tag="IV334_001522"
     CDS             2781..4667
                     /locus_tag="IV334_001522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HAP8943595.1"
                     /translation="MLKELKVTDLKKTYGEKDLFDQISFLVHEKDRIGLIGTNGTGKT
                     SLLNIIAGIDSSDGDRQTVFYPTDYRIGYLSQAAEFSDELTVLQAVFQGNSPLIQTVR
                     AYEEALIELGENGEDPDVQKRYAKAEEQMNKEDAWTTDTNAKIILQKLGISELDKKIS
                     TLSGGQKKRVSLAQVLIDEPDLLLLDEPTNHLDYEAIEWLENYLKQYRGALLMVTHDR
                     YFLDRVANRIFELSFGKLYEYKGNYEAYVLEKAERDRVEVEQEEKRKRLYKQELAWMR
                     AGVQARGTKQQARINRFEDLKENLYQVNQEDDLELNLATQRLGKKVLEIKDGSYRIND
                     QTLLEHLDLLIQSRERLGITGKNGAGKSTLLNILAGRIPLDSGTMSIGETVHLAYYTQ
                     ENEEMAPDKRMIAYLQEAAEEAKTADGSQIGVAELLERFLFPRFMHGTLIGKLSGGEK
                     RRLFLLKLLIQQPNVLLLDEPTNDLDIATLTILEDYFRSFPGAVITVSHDRYFLDKVA
                     DKLLVFQGNGKQELYYGNMSSYLLKQKETQQPAEKAKPKIQSKEPAEKKKLSYMEQKE
                     WETIEDEIAELEEKISLLQEEMNHQGDNFTRLQELQNDVSETEAQLEEKMARWEYLSE
                     WVED"
     gene            4679..5626
                     /locus_tag="IV334_001523"
     CDS             4679..5626
                     /locus_tag="IV334_001523"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HAP8943596.1"
                     /translation="MEQAYLDLGRKLLEEGHRKEDRTGTGTLSLFGYQMRFDLQKGFP
                     LLTTKRVPFGLIKSELLWFLKGDTNIRYLLQNKNHIWDEWAFERYIKSPDYNGPDMTD
                     FGHRCLTDPEFNEEYKKQSKLFCEKILTDDSFAETYGDLGHIYGYQWRHWETKDGGFI
                     DQIKQVIEAIKKTPDSRRLIVSAWNPEDVPSMALPPCHTMFQFYVQEGRLSCQLYQRS
                     GDVFLGVPFNIASYALLTHLIAHETGLAVGEFVHTLGDAHLYINHIEQMKEQLSREIR
                     AFPTLVLNQEKQSVFDFEMEDIQLEGYDPHPAIKAPIAV"
     gene            5646..6173
                     /locus_tag="IV334_001524"
     CDS             5646..6173
                     /locus_tag="IV334_001524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HAP8943597.1"
                     /translation="MLAAIWAQDENGLIGKEDRLPWRLPNDLKFFKQMTEANTLVMGR
                     KTFEGMGSRPLPNRQTIVLTRDSSYKAEGVHVMHSVEDVLALEKETDGIFFIAGGSAV
                     YQEFLPFCTILYRTVIHHVFDGDAYFPPVDWSDWSLINTSQGEMDEKNRYPHQFETYQ
                     RNENPVKSGTGELTI"
     gene            complement(6236..6889)
                     /locus_tag="IV334_001525"
     CDS             complement(6236..6889)
                     /locus_tag="IV334_001525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HAP8943598.1"
                     /translation="MEKTKFNRTYEILNEVFNAVTHGIGTGLSIAGLVVLLVKGAHLH
                     SSLHIVSYAIYGSMMILLFLTSTLFHSLIFTKAKKVFQVFDHSSIFLLIAGSYTPFCL
                     LSIRGWLGWTLFVLIWLFAILGIIYKALTLHKRERVSKVSTIIYIFMGWLCVVAAVPL
                     YHSLGMTGVILMVAGGLSYTIGAFFYSLNSIRYMHVVWHLFVMLGAGFMYFSVLFFT"
     gene            7021..7863
                     /locus_tag="IV334_001526"
     CDS             7021..7863
                     /locus_tag="IV334_001526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HAP8943599.1"
                     /translation="MTNVKIVTDSSCTMEKNVRDALGIRVMPLSIMIDGTVYPDDDQL
                     DGERFMEMMAKAKNLPKTSQPPIGEFISMYDELGEDGSEVISIHMTKGLSGTVEAARQ
                     ASNLTKTKVTVIDSDTTDQGLSFQVIRAAELAKEGKTVEKILEEVKKVQDNTKLYIGI
                     STLDNLVKGGRISRTTGLLSSVLNIRVVMNFAHSELIPVTKGRGKKTFDKWFNNLKNE
                     LKELPNVRQIGISHADAKELAQEFKEGLQELFPDMHIPVLHTNPVIATHTGKDAFAIM
                     YYVD"
     gene            8021..8917
                     /locus_tag="IV334_001527"
     CDS             8021..8917
                     /locus_tag="IV334_001527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HAP8943600.1"
                     /translation="MKLTKRHLILVLFPLLIAIIIYAGLAASIPKAGPLMKPVTTVRE
                     DPNKKEKLHYIAIGDSLTEGVGDQTKQGGFVPLVANDLKDRYNLTAVETENYGVNGER
                     SDQILKRVKTKEEIRNNIKTADFITLTVGGNDLMKVIQNNLFGLTVNSFKKPLKNYQE
                     NVTELLEEFRSLNKTAPIYILGIYNPFYLNFPEITDMQTIVNNWNKGTETVTKKMPNC
                     YFIPINDLLYQGLNQEVGVTEEGTDQTTSTTENLNGIKNNVLYEEDHFHPNNLGYQLM
                     ANAVRDQLIETQNQWLEKEDDQ"
     gene            8920..9633
                     /locus_tag="IV334_001528"
     CDS             8920..9633
                     /locus_tag="IV334_001528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpmS family protein"
                     /protein_id="HAP8943601.1"
                     /translation="MKTRSDVSSNKTKKTNYWKFAFLILLGVVLGSTVFLGSRIFANR
                     EPELPEIPALTERQGDPVLTINSNKEKVNQIISFFLSEYQKDSDIEYKFYLENEALLN
                     GTFEVLGFPIDFYLYFDPYVMENGNVQLKARSLSIGTLSLPIKDVMNMIKRNYKLPDW
                     IEVNTDDLTIMLRLDQFRMQNGMYIKADKIDLVNDDIRFSLYLPKDTSDASNNSNTNK
                     TSTSSTESKTKENKKSSDE"
     gene            9653..10171
                     /gene="msrA"
                     /locus_tag="IV334_001529"
     CDS             9653..10171
                     /gene="msrA"
                     /locus_tag="IV334_001529"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HAP8943602.1"
                     /translation="MERAIFAGGCFWCMIQPFDTLPGIHTVMSGYTGGHVPDPTYEQV
                     KSQTTGHTEAVEIIYDPTLISYEQLVELYWQQTDPTDAFGQFEDRGDNYRPVIFYLTE
                     EQRIIAEKSKKQLAESGRFEQPIVTKIEPAQPFYLAEEDHQEYYKKHPENFKRNHARR
                     AAFIEENWRNEG"
     gene            10168..10395
                     /locus_tag="IV334_001530"
     CDS             10168..10395
                     /locus_tag="IV334_001530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YozE family protein"
                     /protein_id="HAP8943603.1"
                     /translation="MNRSFFHYLMTLRGGGTHDALSVFATEAGKDIQFPKHSSSYEEI
                     SNYLELNVDYLPSMDIFDEAWEKYLENNHDI"
     gene            10547..11098
                     /gene="lepB"
                     /locus_tag="IV334_001531"
     CDS             10547..11098
                     /gene="lepB"
                     /locus_tag="IV334_001531"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HAP8943604.1"
                     /translation="MTKKQRYINRFWLVFKYLLVSVFLAFMLRGFLFIPVPVEGNSME
                     NVLKQGDMVVMEKFSEIRRFDIVVFQLADGTIYIKRVIGLPGENVSYQNDQLKINGKV
                     VKEPYLTKNLKSDHANASYTTDFTLQELTGQSKLPEDNYFVLGDNRRVSKDSRSFGTI
                     NKTDILGKARFVYYPLDEIKWIQ"
     gene            complement(11181..11723)
                     /locus_tag="IV334_001532"
     CDS             complement(11181..11723)
                     /locus_tag="IV334_001532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="folate family ECF transporter S component"
                     /protein_id="HAP8943605.1"
                     /translation="MNTLIAKDVFRLPEMNARLLSTSALLLALDVILYKIAIGPAYFQ
                     ITFGFLSMALMGYLYGPIWAGLLEMLSNTLNFTIFGSGTFQAAFLITAFLGGFINGLF
                     LYHKKINLPSVIAAQLLIMIPVSLFINSWLMSVLFGADFKVIFLGRFIRNMIQIPIQI
                     MVVYSFIKTLEARKVFKRAV"
     gene            complement(11960..12358)
                     /locus_tag="IV334_001533"
     CDS             complement(11960..12358)
                     /locus_tag="IV334_001533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase"
                     /protein_id="HAP8943606.1"
                     /translation="MNSMVFVNFPVADVNRSVAFYEKLGFKKNEEFSTEEAGSMVWDD
                     TFWIMLLSHDFYKKFIKDKEIADNKKTSGALISFSVENADEVKKFAQIAKENGGTFHE
                     VDMGMPEEEMFSLEVQDLDGNTLEPVWMKM"
     gene            12618..13478
                     /gene="ylqF"
                     /locus_tag="IV334_001534"
     CDS             12618..13478
                     /gene="ylqF"
                     /locus_tag="IV334_001534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YlqF"
                     /protein_id="HAP8943607.1"
                     /translation="MTIQWFPGHMAKARREVSEKMKYVDIVFELVDARLPLSSRNPML
                     DQIIQQKPRLVLLNKADLADPQQTKLWQEYFRKQGHLALAITAQESKGIKGLVPAAKE
                     ALKEKRARDAARGIKPRAIRAMVLGIPNVGKSTLMNRLVGKKIAQTGNKPGVTKGQQW
                     LRLGNELELLDTPGILWPKFEDEEIGKKLALTGAIKDQLVHLDDLAIYGLDFFARYYS
                     GRISDRYGLSIEQEEKLAPELLMDITAKRGYKEDYDRGSELVVHDIRQGKLGRYTLDR
                     CEEMEQKSDD"
     gene            13471..14238
                     /locus_tag="IV334_001535"
     CDS             13471..14238
                     /locus_tag="IV334_001535"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HAP8943608.1"
                     /translation="MTESIQSIKERLKSIQRIDDPTLESLRNDPRKGVQQALASFERR
                     LKKEALIKEKYEEMFCFEKQGYAQGHQWIAGIDEVGRGPLAGPVVAAAVILPKNEIIL
                     GLNDSKQLSEKKRELLYQQVQEKALAIGIGIVDQQTIDDVNIYEASKLAMIQAVEKLS
                     IVPSYLLIDAMTLDLPIQQEKIIKGDARSVSIAAASIIAKVYRDQLMKEYDVLYPGYG
                     FSHNAGYGTKEHLEGLERQGITPIHRKSFAPVKKYLQ"
     gene            14294..15151
                     /gene="dprA"
                     /locus_tag="IV334_001536"
     CDS             14294..15151
                     /gene="dprA"
                     /locus_tag="IV334_001536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-protecting protein DprA"
                     /protein_id="HAP8943609.1"
                     /translation="MYQIEENLLKLAYTKGISSQGKWALANWMMQYPYKEIGFEDIIK
                     IGKITKHRELFRKSWQEIHANWGKIQSKQSFITCFDPKYPPQLLHLTYPPIVLFYNGD
                     LSLLSCNMLSVVGGRHTSALAKKTVYYLLKPVIEAGYVIVSGCAKGIDTYGHQAAISH
                     GGRTIAVIGTGIEQVYPAENRDLQEVIANDHLLLSEYPPQSGPKKHCFPMRNRIIAAL
                     SKGTCVFEAKKKSGSLITAQQALDLGRTVFSVPGNILTEHSTGCHHLIQDGAVCTVSG
                     EDILAEFKE"
     gene            15273..17351
                     /gene="topA"
                     /locus_tag="IV334_001537"
     CDS             15273..17351
                     /gene="topA"
                     /locus_tag="IV334_001537"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002380330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HAP8943610.1"
                     /translation="MAYKYLVIVESPAKAKTIEKYLGRNYKVMASVGHIRDLPKSKMG
                     IDFENNYEPHYISIRGKGDVIKSLKAAAKKAQKVYLASDPDREGEAIAWHLAYLLGLD
                     LKEKNRVVFNEITKDAVKAAFKEPRTIDVDLVDAQQARRTLDRIVGYSISPILWRKVK
                     KGLSAGRVQSVALKIIIDREKEIREFVPEEYWSIDGNFKKARKKFKANFWGIDGKKKK
                     LPDAQSVKEVTARIDGTDFEVKKVEKKERKRNPANPFTTSSLQQEAARKLNFRTRKTM
                     MVAQQLYEGITLGKQGTVGLITYMRTDSTRIADTAKAEAAAFIEDTYGKEFSRHDHRK
                     IKNAQGAQDAHEAVRPTSVLRTPDELKQYLDKDQLKLYTLIWSRFVASQMTPAILDTM
                     KVTLEQNGVIFIANGSKIKFKGFMQVYVEGRDDGKEEKENILPELEEGDVVQSVDIEP
                     KQHFTQPPARFSEATLIRALEENGVGRPSTYAPTLDTIQRRYYVKLTQKRFEPTELGE
                     IVNSLICEFFPQIVDIHFTAEMEGDLDKIEEGTEAWVKVVDRFYKPFEKELTNAEEKI
                     EKIQIKDEPAGFDCDVCGHPMVIKLGKYGKFYACSNFPDCRNTKPIVKEIGVTCPVCH
                     EGQVIERKSKKNRLFYGCSRFPECEFTSWDKPVGRNCPKCDGYLVEKKVKGGKQVVCV
                     NGDYEEDIQK"
     gene            17320..18696
                     /gene="trmFO"
                     /locus_tag="IV334_001538"
     CDS             17320..18696
                     /gene="trmFO"
                     /locus_tag="IV334_001538"
                     /EC_number="2.1.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FADH(2)-oxidizing
                     methylenetetrahydrofolate--tRNA-(uracil(54)-C(5))-
                     methyltransferase TrmFO"
                     /protein_id="HAP8943611.1"
                     /translation="MEITKKTSKNNRKEVLSLKMVNVVGAGLAGSEAAWQIAQAGVPV
                     RLYEMRPVKSTEAHHTSNFAELVCSNSLRGNSLANAVGLLKEEMRRLNSVVITSADET
                     AVPAGGALAVDRDSFSETITRKVKEHPLVTVINEEITEIPEGITVVATGPLTSHPLAE
                     SIKAFNGSDGFYFYDAAAPIVDKATIDMDKVYLKSRYDKGEAAYLNCPMTEEEFKAFH
                     EALINAEVAELKSFEKEKYFEGCMPIEVMAQRGFKTMLFGPMKPVGLEDPKTGKRPYA
                     VIQLRQDNAAASLYNIVGFQTHLKWGEQKRVFRMIPGLENAEFVRYGVMHRNSFMNSP
                     ELLKPTYQSTKREDLFFAGQMTGVEGYVESAASGLLAGINAARLAKEMEPVEFPQETA
                     IGSMAYYITHAEGKHFQPMNANYGLFPELPERIRDKKARYEAIAQRALKANEAIKTEL
                     EKTAIPNE"
BASE COUNT         6518 a         3231 c         3899 g         5092 t
ORIGIN      
        1 ttttgtcgca gccccatttt tttgttgtga taaaagaaca agaaaggcag gaatgggaaa
       61 gtaaacatcc ttttgtcaga aacttttatc tttcttaagt tttacttgat atctgatact
      121 atttggaata gaatgaggcg ttgaaaaatt tatggaaatt tctaactagc aataaacgat
      181 gaaacaatga aatatttttc atccaaaaag aactttgcta taatgatgga agtttgtgta
      241 taggagatct ggaaaatgag agagagtttt gaacaacaaa aaaaactgtt gcatgatcgc
      301 tatggcgccc tttcgatgga tgaccgccgt caaatacttt gtaagttgcg taaaagaaat
      361 atattgatgt atcgtcagtt ggaacgtctg aaacatgatc tgcttcgttt ggagtcaaaa
      421 cgtgtacagt gtgaattaga ggggaaccaa acacaggttg aagtagtcga aaccaaaatt
      481 ttaaagaaaa aagaacaatt tttaaaaatg cttacacaaa ataaaaaata aaggggatgg
      541 acgatggaat ttgctgagtt agttattgta tttgcagtaa cgatcacctt atcgaacata
      601 gctagccgca ttttgccaat gattccagct ccactgatcc aaattttcct tggagtggta
      661 ttaggtctga cagagtgggg acaatcaatc gattttgaac ctgaattgtt cttagttatg
      721 attattgcac cattgttatt tagagaagga gaaaaagcag atatttcttc gattctcaaa
      781 aatttcggga caattttgtt cttggcattt ggaggagtca ttttgaccct tgtcggggta
      841 ggtgcgacgt tatcattcct gttgccaagt gtcccacttg ctgcctgctt tgcttttgga
      901 gcagcgttag gtccgacaga tgcggtagca gtcagttctc tttctggacg agtgaatatc
      961 cctaaaaagg cgatgcatat tttggaagga gaaggtcttt tgaatgatgc atctggtgtt
     1021 actgccttcc agtttgctct tggtgcgctg atcaccggat ctttttcagc agtaaacgcg
     1081 gggatgagtc tagtagtttc tagtattggg ggagctttga tcggcttttt gcttgtctgg
     1141 ttcaaacaaa aaataattca tttgatcgaa aaagcatcag cacaagatgt cacagggtat
     1201 ctgctgatcg aacttttgct tccttttcta gcctatgtat tggcagaggc atttgatgta
     1261 tctggaatca ttgctgcagt ggcagcaggt attctccaag cttctggttt taggaaaatc
     1321 tctgtgtttg atgcagaatt gtcgagcttg tcccacagta catggacaac tattgctttt
     1381 acactgaatg cattagtatt tatattttta ggaatcgaat tgacgcaagt gttctcgcca
     1441 gtatggggag acggtttata tccaaacggt cttttattag cgattatcgt attaatctct
     1501 gtcatgcttt ttgtgatcag attcatttct atcagtcttt tttatgtatt caaagatggc
     1561 agtaaaaagt tcaaaaaaca gttgaacgag atcttgatct taacatttgg cggtgtaaag
     1621 ggaacagtca gtttggctac gatttttatt ttgccatttt caatcaataa tatgatgttt
     1681 taccagcggt ctttgctttt gtttctgacg gcgggtgtca tcttagtgac tttggtcata
     1741 gggatcattg tcttgcctat gctgacggaa acagaggaag cgaaaagcac ggatttgaat
     1801 gctttgatga ttttagaaga ggtcgttgaa gttttgcgaa aagaaataaa ggaaattgat
     1861 cgtaatacaa aagaattttt agccactgaa gcagtgatag aaaattatca agaacggata
     1921 cgtgatctgt acttggaaga tttaaccgat gatgaaaagc aagaagtaca agaaatacaa
     1981 gcactgattt tgtcgatcga acgggatgga ttggatgaaa gttatcgttc gggcaagctt
     2041 tcttctaatg gttatcgttt ttactcccgt tttctttctc gattcgaaca ttctatcaca
     2101 agccagattt tgtcttttat cggcttttgg ttcattgttg tccgtaggtt aatgcgaatc
     2161 gttctccatc cgaaaatgtt ttggcaaaga agatatgctg atcgtcagac atttattagt
     2221 gatgaagata tccaagaaat cagagagaca tatcaaaaaa atactcagtt gattattgaa
     2281 agtttagata acttgactga tatttatgat gaaacgatgg ttcaattttt tatccagcaa
     2341 cgcaaacttg aaggaataaa aatgaactcg gggaatttga tttcttcgtg gatgatccag
     2401 caagatagtc tgtttacgaa aaaaatgcta agaggttatt atcttgaaag aaaaatcatt
     2461 gatgaatacg aagttgctga aaaaatcacg acgttttctg cgaacagcta tcgcaggaat
     2521 atcaatttgt tagaatctta tacgatgaac aaaccatctg ataacttttc tttccgattt
     2581 gcgtttcgag caaagtcaaa aaaacgagtc caataaaata aaaaggtaaa aattttcaag
     2641 ttttataaaa aacaaaacat atcagaacag gatcgaatcg gtcctgttct ttttttgaga
     2701 acacaaaaag aacaaatacc aaaaaaattt tcctgaagtt cactattttt atgatacaat
     2761 atccaagata aggagggaac atgttgaaag aattaaaagt aactgacctc aaaaaaactt
     2821 atggtgaaaa agatttgttt gaccagattt cttttttggt ccacgaaaaa gaccgtatcg
     2881 ggttgattgg gacgaacggg acgggtaaaa caagtctctt aaatattatt gccggtatcg
     2941 atagcagcga cggtgatcgg caaacagtgt tttatccaac tgattaccgt attggttatc
     3001 tttctcaagc agctgaattt tccgatgaat taactgttct ccaagcagtc tttcaaggaa
     3061 acagcccctt gatccaaact gttcgagcat atgaggaagc attgatcgaa ctaggggaaa
     3121 acggagaaga ccctgatgtg caaaaacgtt atgctaaagc agaagaacaa atgaataaag
     3181 aagatgcttg gactacagat accaacgcaa agattatttt gcaaaaatta ggaatcagtg
     3241 aattagataa aaaaatcagt actttatcag gtggtcagaa aaaacgagtt agtctggcgc
     3301 aagtgttgat tgatgaaccc gatttactat tactagacga accaacaaac catttagatt
     3361 atgaagcgat cgagtggttg gaaaactatt tgaaacaata ccgtggcgca ctattgatgg
     3421 ttacccacga tcgttatttt ctagatagag tagcaaaccg aatcttcgaa ctttcatttg
     3481 gaaaattata cgagtataaa ggaaattatg aagcttacgt cttagaaaaa gcagaacgtg
     3541 atcgtgtaga agtagaacaa gaagaaaagc gcaagcggct ttataaacag gaattagctt
     3601 ggatgagggc tggcgtacag gcacgtggca ccaagcagca agcacggatc aaccgtttcg
     3661 aagatctaaa ggagaacttg taccaagtaa accaagaaga tgatttagag ctgaacctag
     3721 cgacacaacg tttagggaaa aaagtactgg aaatcaaaga tggcagttac cgtataaatg
     3781 accaaacact gctagaacat ttagatttat tgattcaatc tcgggaaaga ctaggtatta
     3841 ctggtaaaaa tggtgcggga aaatcgacat tgcttaatat tctagctggc agaattcccc
     3901 tggatagcgg tacaatgtct atcggtgaaa cggttcatct tgcgtattat acacaagaaa
     3961 atgaagaaat ggcaccagat aaacggatga ttgcttactt acaagaagct gctgaagaag
     4021 caaaaacagc agatggcagt caaatcggag tagcagaatt acttgaacga tttttatttc
     4081 caagatttat gcatgggaca ctgataggca aactttcagg cggagaaaaa cgccggttat
     4141 ttttgttgaa actgctgatt cagcaaccta atgttttatt attggatgaa ccgaccaatg
     4201 atttggacat agcaacattg acgattttgg aagattattt tagaagtttt ccaggtgctg
     4261 tgatcactgt ttcccatgac cgttatttct tggacaaggt agcagataag ttattagtat
     4321 tccaaggaaa cggaaaacaa gaactttatt acggaaacat gtccagttat ttactgaagc
     4381 aaaaagagac ccagcagcct gctgaaaaag cgaaaccaaa aatacagagc aaggaacctg
     4441 ctgaaaagaa gaaattatct tacatggaac aaaaagaatg ggaaacgatt gaagatgaga
     4501 ttgcagaact tgaagaaaaa atctcattgc tccaagaaga gatgaatcat caaggagata
     4561 atttcactcg attacaagaa ttgcaaaacg acgtatctga aacagaagca caacttgaag
     4621 aaaaaatggc acggtgggaa tacttgagtg aatgggtaga agattaggag gaaaaaggat
     4681 ggaacaagcg tatcttgact taggaagaaa attgttggaa gaagggcatc gtaaagaaga
     4741 cagaacaggc acaggcacat taagcttatt cggataccaa atgcggtttg atctgcaaaa
     4801 agggtttcct ttgttaacaa cgaaacgagt accttttgga ttgatcaaaa gcgaattgct
     4861 ttggttttta aaaggagata cgaatatccg ctatctatta caaaataaaa accatatttg
     4921 ggatgaatgg gcatttgaac gctacatcaa aagtccagac tataatggac cagatatgac
     4981 tgattttggc catagatgtt tgacagatcc tgaattcaat gaagaatata aaaagcagag
     5041 caagcttttt tgcgagaaga tcctaaccga tgattccttt gctgaaacat acggcgatct
     5101 tggacacatc tacggctatc aatggcgtca ctgggaaaca aaagacggtg gctttatcga
     5161 tcaaatcaaa caagtgatcg aagcaatcaa aaagactcct gattctagaa gattgatcgt
     5221 ttctgcatgg aatccggaag atgttcctag catggctttg cctccttgtc atacgatgtt
     5281 tcaattttat gttcaagagg gacggctaag ctgccaattg tatcaacgca gtggggatgt
     5341 gtttctaggc gttccgttca acattgcaag ttatgcgttg ctgactcacc taattgcaca
     5401 tgaaacaggt ttagcggttg gcgaatttgt tcacacatta ggagatgctc atctttatat
     5461 caatcatatc gaacaaatga aagaacaatt gagccgagaa atacgcgcat ttccgacttt
     5521 agttttgaat caggaaaagc agtcagtatt tgattttgag atggaagata tccaattaga
     5581 aggatatgac ccacatccgg cgatcaaagc tcctatcgct gtttagaatc agaaaggatg
     5641 agaaaatgtt agctgcaatt tgggcacagg atgaaaacgg attgattgga aaagaagacc
     5701 gtcttccttg gcgtttgcca aatgatctga aattcttcaa acaaatgaca gaagcaaata
     5761 cactagtgat gggacgaaaa acattcgaag ggatgggtag ccgtccgttg ccgaatcgtc
     5821 agacgattgt tttgacgaga gattcttcct ataaagcaga aggcgtgcat gtcatgcata
     5881 gtgtggaaga cgtgcttgct ctggaaaaag aaacagacgg tatctttttc atcgctggag
     5941 ggtcagctgt ttatcaagaa tttctgccat tttgtacgat attataccgt acagtcatcc
     6001 atcatgtatt cgatggagat gcttattttc cacctgttga ctggtctgat tggtcactga
     6061 tcaatactag tcaaggagaa atggatgaaa aaaatcgtta tccgcatcaa tttgaaacgt
     6121 atcagcgtaa tgagaatcct gtaaaaagcg ggacaggcga attgacgata taaaaaagct
     6181 atggatagac tagctgttat ttagagctgt cccatccata gctttatttt ttttattaag
     6241 taaagaataa aacagagaag tacataaaac ctgcacctaa cataacaaac aaatgccaaa
     6301 caacatgcat atagcggata gaattcaagc tataaaagaa tgcgcctatc gtgtaagaaa
     6361 gcccgcctgc taccatcaaa ataacaccag tcattccaag agagtgataa agaggaacag
     6421 cagccacgac acagagccac cccataaaaa tatagatgat cgtagatact tttgatactc
     6481 tctcccgttt atgcaacgtc aacgctttat atataatgcc aagaatcgca aacaaccaga
     6541 ttaatacaaa taaagtccaa cctagccatc ctcggatact cagcaaacaa aaaggagtat
     6601 aacttcctgc aatcaaaaga aaaatcgaac tatgatcgaa tacttggaac accttttttg
     6661 cttttgtaaa aatcaatgaa tgaaacaaag tagacgttaa aaataaaaga atcatcatcg
     6721 atccatagat agcataagag acaatatgga gggaggaatg caagtgggca ccttttacta
     6781 aaagaaccac tagtcccgcg atactcaatc cagtcccaat tccatgggtg actgcattaa
     6841 acacttcatt taatatttca tacgttcgat taaatttcgt tttttccatg aaaacggctc
     6901 ctttactagg tgtaattaat gaaaaatcat tatttgtctg ttatactaaa aatagaaatt
     6961 gtcttacatg tagtttaaca tgaataaggc atcttgttaa agaaagagag agagaaaata
     7021 atgacaaatg taaaaatcgt aacagattct tcatgtacga tggaaaaaaa tgtacgcgat
     7081 gctttaggca ttcgtgtcat gccgctatcc atcatgatcg acggaacagt atatcccgat
     7141 gatgaccaac tagacggcga aagatttatg gaaatgatgg cgaaagcaaa aaatctgccc
     7201 aaaacaagcc aaccaccaat tggagaattc atctcgatgt atgatgagct aggagaagac
     7261 ggcagtgaag tgatttcgat ccatatgaca aaaggcttga gtggaacagt agaagcagca
     7321 agacaagcca gtaatctaac aaaaacgaaa gtaacggtta tcgacagtga taccactgac
     7381 caaggattat cttttcaggt aattcgagca gctgaattag caaaagaagg taaaacagta
     7441 gagaaaatcc ttgaagaagt caaaaaagta caagacaata caaaattata catcggaata
     7501 tccacactag ataacttggt aaaaggtgga agaatcagcc gaacaactgg tttgctgtct
     7561 agtgttttga acatacgcgt ggtgatgaat ttcgcccatt ctgaattaat ccctgtaact
     7621 aaggggcgag gaaagaaaac gttcgataaa tggtttaata atttgaaaaa tgaactgaaa
     7681 gaattgccaa atgtgcgaca aatcggtatt tcacatgcgg atgcaaaaga attggcgcaa
     7741 gaattcaaag agggtttgca agaattattc ccggacatgc atatccctgt actgcatacg
     7801 aatccggtta tcgctacgca tactggtaaa gacgcgtttg caatcatgta ttatgtagat
     7861 tagtcgaaaa gaggataagc cctcagcctg aaaggcttgc agcttattct ctttttcttt
     7921 ataaaattag gaaaataatt gttttctttt ttcataaaga ctaccttttt gataaaattt
     7981 ttgataacat tagaaccaat cgaatacaag aggtgaaaaa atgaaattaa cgaaacgaca
     8041 tttgatcctc gttttgttcc cattattaat tgcgatcatc atctatgcag gtttagctgc
     8101 ttctataccg aaagctggcc cactaatgaa accagtaact actgtgagag aagatccaaa
     8161 taaaaaagaa aaacttcact acatagctat cggagattcg ctgactgaag gagtaggaga
     8221 tcaaaccaag caaggaggat tcgttcccct tgtcgcaaat gatctaaaag accgttataa
     8281 tttgaccgcg gttgaaacag aaaactatgg tgtaaatggt gaacgaagtg accagatctt
     8341 aaagcgagta aaaacaaaag aagaaattcg aaacaatatc aaaacagctg attttatcac
     8401 actgactgtt ggcggtaatg atttgatgaa agttatccaa aataatttat tcgggttaac
     8461 ggtcaattcc tttaaaaagc cactgaaaaa ctatcaggaa aatgtcacag aactattgga
     8521 agaatttcga tccctcaata aaaccgcacc tatttacatt ttaggcatct acaatccatt
     8581 ttatttgaac tttcctgaga tcactgacat gcagacaatt gttaacaatt ggaacaaagg
     8641 aacagaaacg gtgacaaaaa aaatgccgaa ctgctacttt atcccaatca atgatttgct
     8701 ttatcaagga ttgaatcagg aagtaggtgt cacagaagaa ggaacagatc aaacaactag
     8761 cacgacagaa aatctaaatg gcatcaaaaa taatgtgctc tatgaagaag atcatttcca
     8821 tccaaataat ttaggttatc aattgatggc aaatgcagtg agagatcagc tgatcgagac
     8881 acaaaaccaa tggttagaga aggaggatga tcaataaaca tgaaaacaag atctgacgta
     8941 tcttcaaaca aaacaaagaa aacaaattac tggaaattcg ccttccttat ccttcttggg
     9001 gtagtacttg gttcgaccgt atttttaggc agccgtattt ttgctaatcg ggaaccagaa
     9061 ctcccagaaa taccagctct tacggaaaga caaggagatc ctgtcttaac gatcaattca
     9121 aataaagaaa aagtcaatca aatcatcagc tttttcctta gtgagtatca aaaagatagt
     9181 gatattgaat ataaatttta tttggaaaat gaagcactac tcaatggtac tttcgaagta
     9241 ctaggttttc ctatcgattt ctatttatat tttgatccgt atgtgatgga aaacggaaat
     9301 gtccaactaa aagctagaag tctttctatc ggaacattga gtttgccaat caaagatgtg
     9361 atgaatatga tcaagagaaa ctataaatta ccagattgga tcgaagtcaa tacagatgat
     9421 ctgactatca tgcttaggtt ggatcaattc cgaatgcaaa acgggatgta tattaaagca
     9481 gataaaatcg acttagtaaa tgatgacatt cggtttagtc tatatttgcc aaaagatacc
     9541 tctgatgctt caaacaactc aaataccaac aaaactagca ccagcagtac tgaaagcaag
     9601 acaaaagaaa ataaaaaatc atcggatgag tagtattaga gggggaatca taatggaacg
     9661 agcaatattt gcaggaggct gcttctggtg tatgatccag cctttcgaca cgcttcctgg
     9721 tatccataca gtgatgtcag gatatactgg agggcatgta ccagatccga cttatgaaca
     9781 agtaaaaagc caaacgactg gacatacaga agcagtggag attatctatg atccaacatt
     9841 gatttcatat gaacaattag tagaacttta ctggcagcaa actgatccaa ctgacgcttt
     9901 cggacaattc gaggatcgcg gtgacaatta tcgcccggtg atcttttatt tgacagaaga
     9961 acagcggatc attgctgaaa aaagtaagaa acagttagct gaaagtggac gtttcgaaca
    10021 gccaatcgtt acaaaaatcg aaccagccca accattttat ctagcagaag aagaccatca
    10081 agagtattat aaaaagcatc ctgaaaattt taaaagaaac catgcacgcc gggctgcctt
    10141 tatcgaagaa aattggagga acgaagggtg aatcgaagct tttttcatta tttaatgaca
    10201 ctgcgaggcg gtggcacaca tgatgcttta tctgttttcg ctacagaagc cggtaaagac
    10261 atccaatttc ctaagcacag ctctagctac gaagaaattt ctaattatct ggaactgaat
    10321 gtcgactatt tgccgtccat ggatatcttt gacgaagcat gggaaaaata tttagaaaat
    10381 aatcatgaca tataagagat agttttgtac tttatgatca aaaaagtttt ttgtccaata
    10441 gcctaattga aagaattatt tttatttcag ctatctagtc gcattttttt gatatttttc
    10501 ttataataaa gaaagaatga aaaaaagagg gatggattga tagagattga caaaaaaaca
    10561 acgctatatt aatcgcttct ggcttgtatt caaatactta cttgtttctg tattccttgc
    10621 gttcatgttg cggggatttt tattcatccc ggtaccagtg gaaggcaatt caatggaaaa
    10681 cgtgttgaag caaggtgata tggttgtgat ggaaaaattt tcggagatca gacgatttga
    10741 tattgttgtt tttcaattag cagatggcac gatttatatc aaacgagtaa tcggtcttcc
    10801 aggagaaaat gtcagctacc aaaatgatca attgaagatc aatgggaaag tagttaagga
    10861 accttatcta actaaaaacc tgaaatcaga tcatgcaaat gcatcctata cgacggactt
    10921 tacactacaa gagctaacgg gacaatccaa gcttccggaa gataattatt ttgtacttgg
    10981 agataatcga agagtttcaa aagatagtcg ttcgtttggc acgatcaata aaactgatat
    11041 tctaggtaaa gcaagatttg tctattatcc acttgatgaa ataaaatgga tccagtgaga
    11101 aacaataaag cgtgcagtct gttgagactg cacgctttat tgtttaatcc aaaatcagtt
    11161 tactacctta gtacgtaaaa tcaaacagct cgtttaaaca cttttctagc ctctaaagtt
    11221 ttgataaatg aataaacaac cattatttga atagggattt ggatcatatt cctgatgaat
    11281 ctacctagaa aaattacttt gaaatctgca ccaaacaaaa cagacatcag ccaagaattg
    11341 ataaaaaggc tgactgggat cataatcaat aattgggcag cgatgacgga aggcaggttt
    11401 atctttttat gatataaaaa taagccatta ataaatccac caaggaaagc cgtgataaga
    11461 aaagcagctt gaaacgttcc tgaaccaaat atcgtgaaat ttaaagtgtt cgacaacatt
    11521 tcaagcaaac cagcccagat cggtccatat agataaccca taagcgccat acttaaaaat
    11581 ccgaatgtga tttgaaaata agcaggacca atcgctatct tgtataagat gacatcaagg
    11641 gctaataaaa gagcagaggt cgacaacaat cttgcattca tttcgggcaa tctaaaaacg
    11701 tcttttgcaa tcaaagtgtt caaaatattc tctcctttgt cagttaataa taaaaggata
    11761 ccataaaatc accaatgtgt catgacacat tggtgattta catacactgg catctaacaa
    11821 ttaaaaaaag ggtgagaaga ctgcttttat aaactgtaaa aaagagattg tacgaataaa
    11881 aaaaatagag gcggagaata cttatcgtat catccgcctt tgtttttttg ttataaaaaa
    11941 cgtcagatta gttatctgct tacatcttca tccatacggg ttctaatgta tttccgtcta
    12001 gatcttggac ttctaatgaa aacatttctt cttcaggcat tcccatatct acttcgtgga
    12061 aagtaccacc attttctttt gcaatttgag caaatttttt tacttcatcc gcgttttcaa
    12121 cactaaacga aatcaaagca ccacttgttt ttttattatc agctatttct ttatctttta
    12181 taaatttttt ataaaaatca tgagagagaa gcattatcca aaaagtgtca tcccatacca
    12241 ttgaaccagc ttcttcagta gaaaattctt catttttttt aaaacccagc ttttcataaa
    12301 aagcaacgga acgattgaca tcagcaacag gaaaattaac aaaaaccata ctattcattt
    12361 gtttcgtctc ctttctgttt tgttcgtcta tcatctctta tttaggaaac gctgtcaaaa
    12421 atttagattc atatatagaa accaaaataa aaatttgcat catcaaaaaa gtgaagatgt
    12481 gatagaatga ggactggttc actttcactt aactaataga tggaatttct ttataataaa
    12541 aaagtgaatt tatattgaaa aagaaaaaaa caagtaaagc cggggaatat tccggtatga
    12601 aaggaatgga gagaacaatg accatccaat ggtttccggg acatatggca aaagcccgac
    12661 gagaagtttc tgaaaaaatg aaatatgtgg acatcgtatt tgaattggta gacgcacgat
    12721 tacctttatc ttctcgtaat ccaatgctag atcagatcat tcagcaaaaa cctcgccttg
    12781 ttttattgaa taaagcggat ctggcagatc cacaacagac aaagctgtgg caagaatatt
    12841 ttcgtaaaca aggccatctt gcattagcta tcacggcaca agaaagtaaa ggaattaaag
    12901 gtctagtacc tgcggctaaa gaagcattga aagaaaaacg ggcaagagat gctgctcggg
    12961 ggatcaagcc tcgcgctatt cgggcaatgg tcttaggtat tccaaatgtt gggaaatcta
    13021 cgttgatgaa tcgactagta ggcaagaaaa ttgcacagac aggaaataag cctggcgtaa
    13081 caaaaggtca gcagtggctt cgtttaggta atgagttgga attgttagac acaccaggga
    13141 ttttatggcc caaatttgaa gatgaagaaa tcggcaaaaa actagcgctg acaggcgcga
    13201 tcaaagacca gcttgtccat ttagatgatt tagcgattta cggattagac ttttttgccc
    13261 gatattattc aggaagaatc agcgatcgtt atggtttatc aatcgagcaa gaagaaaaat
    13321 tggcaccgga attactgatg gatattacag caaaacgtgg gtataaagaa gactatgatc
    13381 gtggcagcga attagtcgtg catgatattc gtcaaggaaa acttggacga tacacactgg
    13441 atcgttgcga agaaatggag caaaaaagtg atgactgaat ccatccaaag tatcaaagaa
    13501 cgattgaaaa gcatacagag aatagatgat cctacactag aatcattaag aaatgatcca
    13561 cgcaaaggcg tgcagcaagc actggcttct tttgaacgac gattgaaaaa ggaagcatta
    13621 attaaggaaa aatatgaaga aatgttttgc tttgaaaagc aagggtacgc acaaggtcac
    13681 caatggattg ccggcattga tgaagtagga agaggaccat tagctggacc tgtcgtggca
    13741 gctgcagtta tcttgccgaa aaatgagatc atattagggc tgaatgactc caaacaatta
    13801 tcagagaaaa agcgagaact tctttatcaa caagttcaag aaaaagccct ggcaatcggg
    13861 atcgggatcg ttgatcagca gacaatcgat gatgtgaata tttatgaagc aagtaaatta
    13921 gcgatgatcc aagcagtaga aaaattgtcc attgtaccaa gctatttgtt gatcgatgcg
    13981 atgaccttgg atttgcctat tcagcaggaa aaaataatca aaggggatgc acgatctgtg
    14041 tcgattgctg ctgcaagtat cattgctaaa gtatatcgag accaattgat gaaagaatat
    14101 gacgtgcttt atcctggata tggtttctcg cataatgcag gttatggaac aaaggaacac
    14161 ttagaaggac ttgaacgaca aggaattacg ccaatccatc gtaaaagttt tgcacctgta
    14221 aaaaaatatt tacaataaaa aacagccgct tttacgtatc ttattagaaa gagagacgaa
    14281 aggcggtttt atgatgtatc aaatagaaga aaatttattg aaacttgctt atacaaaagg
    14341 aatcagtagt caaggaaaat gggcacttgc taactggatg atgcagtatc cttataaaga
    14401 aatcggattt gaagacatca ttaagatagg taaaataacc aaacatcggg agctgtttcg
    14461 caaaagttgg caggaaattc acgctaactg gggcaaaatc caatcgaaac agtcgtttat
    14521 cacttgcttt gatcccaaat atccccctca gctgcttcac ttgacctatc cgccaattgt
    14581 actgttttat aatggtgatc tatctttact ttcttgtaac atgttaagcg ttgttggtgg
    14641 aagacacact tctgctttag ccaaaaaaac agtttattat ttactaaaac cagtgataga
    14701 agctggatat gtgatcgtca gtggctgcgc gaaagggata gatacatacg gtcaccaagc
    14761 agcaatctct catggtggac ggacaatcgc agtcattgga acagggatag agcaagtcta
    14821 tcctgctgaa aatcgtgact tgcaagaggt gattgcaaat gatcatcttc ttttgagtga
    14881 atatcctcct caatccggcc cgaaaaagca ttgttttcca atgagaaatc ggattatcgc
    14941 agctttgtct aaaggaacat gtgtctttga agccaagaaa aaaagcggct ctctgataac
    15001 agcacagcaa gcattggatc ttggaagaac agttttttct gttccaggaa atattttaac
    15061 agaacattct accggatgtc atcatttgat ccaagacggt gcagtttgca cggtttcggg
    15121 tgaagatatc ttggcagaat ttaaagaata acaccatact tttcatttgc ttttcttgac
    15181 aaacgttttc tgaatggata tgattagcta cgatttgtat gaatcctctt ttaatagtag
    15241 gaacacgaca atggaaagga gtccattcag taatggcgta taaatattta gtgattgtag
    15301 aatcacctgc aaaagccaag acaattgaga aatatcttgg acgaaactat aaagtaatgg
    15361 ccagtgttgg ccatataaga gatttaccga aaagtaaaat gggtatcgat tttgaaaaca
    15421 attatgaacc ccattatatt tctatacgcg gaaaaggcga tgtcatcaaa agcctgaaag
    15481 ccgcagcaaa aaaagctcaa aaagtttact tggcaagtga cccggataga gaaggagaag
    15541 cgattgcttg gcatttagcg tacctacttg ggttggatct gaaagaaaaa aatcgggtgg
    15601 tcttcaatga aatcacaaaa gacgcagtca aagcagcttt taaggaacca agaacgatcg
    15661 atgtagattt agtagatgca cagcaagctc gtcgtacctt agacagaatc gttggttatt
    15721 cgatcagtcc tattctctgg cgtaaggtca agaaagggtt aagtgcagga cgtgtccaat
    15781 ctgtcgcttt aaaaattatt attgaccgtg aaaaagagat ccgagaattt gttccagaag
    15841 aatattggag catcgacggt aattttaaaa aagctcgcaa gaaattcaaa gcaaatttct
    15901 ggggaatcga cggtaagaaa aagaaattac cagatgcaca aagtgtaaaa gaagtcactg
    15961 ctagaattga tggaacagac ttcgaagtta aaaaagtaga aaagaaagag cgcaaaagaa
    16021 accctgcgaa tcctttcact acaagtagcc tgcaacaaga agctgcccgt aagttaaact
    16081 ttcgaacaag aaaaaccatg atggtagcac agcaattata tgaaggaatc actttgggta
    16141 aacaaggaac agtcggcttg attacataca tgcgtactga ttctacccgg attgccgata
    16201 cagcaaaagc agaggccgca gcatttattg aagatacgta tggaaaagaa ttttctagac
    16261 atgatcaccg aaagatcaaa aatgctcaag gcgcacaaga tgcccatgaa gctgtgcgtc
    16321 caacgagtgt gcttcgtaca ccagatgaac tcaaacaata tttagataaa gatcaattga
    16381 aactttatac attgatctgg tcacgttttg tggctagtca gatgacacca gctattttag
    16441 atacaatgaa agtgacatta gaacaaaatg gtgtcatctt cattgcgaac ggttcaaaaa
    16501 tcaaattcaa aggattcatg caagtgtatg tggaaggtag agatgacggc aaagaagaga
    16561 aagaaaatat tcttccagaa cttgaagaag gcgatgtagt ccaatctgtt gatattgaac
    16621 caaaacaaca ttttactcaa ccaccagcac gttttagtga ggctacgttg attcgtgcac
    16681 tagaagaaaa cggggtaggg cgaccttcca cttatgcacc aacacttgat acgatccaaa
    16741 gacgctacta tgtaaaattg acacaaaaaa gatttgagcc gactgaacta ggagaaattg
    16801 tcaattcatt aatctgtgaa ttcttccctc aaatcgtcga tatccatttc acagcagaaa
    16861 tggaaggaga tttggataaa attgaagaag gcacggaagc gtgggtcaaa gtggttgatc
    16921 gtttctataa accgtttgaa aaagaactga cgaatgcgga agaaaaaatc gaaaaaattc
    16981 aaatcaaaga tgaaccagca ggattcgatt gtgatgtctg tggacatccg atggtgataa
    17041 aattagggaa atatgggaag ttttatgctt gcagtaattt tcctgattgt cggaatacga
    17101 aaccaatcgt aaaagaaatc ggtgtgactt gcccagtatg tcatgaagga caagtcattg
    17161 aacgaaaatc taagaaaaac agactattct atggttgttc ccgtttccca gaatgtgaat
    17221 tcacttcttg ggataaacca gtgggcagaa attgtccaaa atgtgacgga tatctggtcg
    17281 agaagaaagt caaaggcggt aaacaagtag tctgtgtaaa tggagattac gaagaagaca
    17341 tccaaaaata atagaaaaga ggttctttca ttgaaaatgg taaatgttgt tggagcaggg
    17401 cttgccggaa gtgaagctgc ttggcagatc gcacaagcag gagtccctgt ccgtttatat
    17461 gaaatgcgtc cagtcaaatc gactgaggcg catcacacaa gtaattttgc tgaactagta
    17521 tgttccaatt cattaagagg aaacagtttg gctaatgcag tagggttatt gaaagaagaa
    17581 atgcgccgat tgaattcggt agtaatcact tcggcagatg aaacagcagt gccagctggt
    17641 ggtgccttag ctgttgaccg cgattctttt tctgaaacaa ttacccgaaa agtcaaagag
    17701 catccattag taacagtgat aaacgaagaa attactgaaa taccggaagg aatcaccgta
    17761 gtagcaacag gacctttgac ttctcatcca ttagcagaaa gcatcaaagc attcaatggt
    17821 tcagatggtt tttatttcta tgatgcagct gcaccaatcg tagacaaagc tacgatcgat
    17881 atggataaag tatatttgaa gtcccgctat gacaaaggag aagcagctta cttgaactgt
    17941 ccgatgacag aagaggaatt caaagctttc catgaggcac taatcaatgc cgaagtggca
    18001 gaacttaaat cttttgagaa agaaaaatat tttgaaggat gtatgccaat tgaagtaatg
    18061 gcacagcgag gattcaaaac gatgcttttc ggacctatga aaccagttgg attggaagat
    18121 cctaaaactg gcaaacgtcc ttatgctgtt atccagctgc gtcaagacaa tgctgcagct
    18181 tctttataca acatcgtggg ctttcagact cacttgaaat ggggcgagca aaaacgtgtt
    18241 ttccggatga ttcctgggtt agaaaatgct gaattcgttc gctatggtgt gatgcaccga
    18301 aacagcttta tgaattcgcc agaattgctg aaaccgactt accaatcaac gaaacgcgaa
    18361 gatctgttct ttgcaggtca gatgactggt gtagaaggct atgttgaaag tgcagcgagc
    18421 ggactacttg ctggaatcaa cgccgcacgc ttagcaaaag aaatggaacc agtcgagttt
    18481 ccacaagaaa cagctattgg aagtatggct tactacatta ctcatgcgga aggaaaacat
    18541 ttccaaccga tgaatgccaa ctatggtctg tttcctgaac tgccagagcg cattcgagac
    18601 aaaaaagcaa gatatgaagc aattgcacaa cgagctttaa aagctaatga ggcaataaaa
    18661 acagaattag aaaaaactgc aatcccaaat gagtaaggaa actccatgaa gagggtgaga
    18721 cggatagtct cgctcttttt
//
LOCUS       DACDKM010000048        18258 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20834_2016
            SAMN12640370-rid9891663.denovo.048, whole genome shotgun sequence.
ACCESSION   DACDKM010000048 DACDKM010000000
VERSION     DACDKM010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640370
            Sequence Read Archive: SRR10040891
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 18258)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18258)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742835.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742835.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:04:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,012
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 29 of 75
            Pseudo Genes (incomplete)         :: 33 of 75
            Pseudo Genes (internal stop)      :: 19 of 75
            Pseudo Genes (multiple problems)  :: 5 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18258
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640370-rid9891663.denovo.048"
                     /isolate="E20834_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(162..1670)
                     /locus_tag="IV334_001539"
     CDS             complement(162..1670)
                     /locus_tag="IV334_001539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1846 domain-containing protein"
                     /protein_id="HAP8943612.1"
                     /translation="MKKIGFDSEKYIEEQSAYILERVNHYDKLYLEFGGKLVDDKHAK
                     RVLPGFEEDAKIKLLQKLKDQAEILICVYAGDIERNKIRGDYGITYDMDILRLIDELR
                     GYGLLINSVVITRYSGQPATKVFINKLERRNIKVYKHSEIEDYPVNVEKIVSEDGFGK
                     NEYIETTKPIVVVTAPGPGSGKLATSLNQLYHESQKGHAAGYSKFETFPVWNVPLKHP
                     LNIAYEAATVDLKDVNMIDSFHFDAYNKVAVNYNRDVETFPVIKRIIEKITGKESVYQ
                     SPTDMGVNRVGFGIVDDTVVQEASKQEIIRRYFQTECDFKKGLIDEEAVNRIKLIMEE
                     VGLRPEDRNVVVPAHDYADKIQAQSPTKDPSAVIAIELPDEVIITGRTSSLMDASAAV
                     ILNAVKHLAHIADDIPLLSPLVLETIQGLKSKTLHSSLDALSINEVLIALSISAVTNP
                     IAQVAYDKLAELEGAQAHSTVMVNKNDEQTLKKLGIDITCAPVYPSENLYYQ"
     gene            1829..2020
                     /locus_tag="IV334_001540"
     CDS             1829..2020
                     /locus_tag="IV334_001540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8943613.1"
                     /translation="MKKRQKKKNAYKHYIRSIFTGYERMLEDPELEQLTFTYLNEETQ
                     LTRDEHQRIHFTTRDLPSK"
     gene            2223..3140
                     /locus_tag="IV334_001541"
     CDS             2223..3140
                     /locus_tag="IV334_001541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="viral A-type inclusion protein"
                     /protein_id="HAP8943614.1"
                     /translation="MKEPDTNANYEPDELEETNADQDTGQESSSFYDIKEKASMNLSQ
                     LLEDLQAFEKAVREEDIPEIYRLYNGRLNEELKRSSNENHEIDQLLMRKIHDRFLQEF
                     PFMEQVENISPTMSYYKIGTYYHDRPTVGIDASLPEIFVLPKIDEEWEKYSQPESTDY
                     DKKINELDAKVITAQTEIERLDEQIKEINRQMTDLNDNKGFLNRKKIEEEIQELEKKK
                     QVLSNEKLGWLPYIETPETIQQQKEALKQEARADQLRAAIVEKEQRQIRRYFGSKEGF
                     GQAIHEFLMNYLGNENPNNQSNEGGSEYE"
     gene            3133..4206
                     /locus_tag="IV334_001542"
     CDS             3133..4206
                     /locus_tag="IV334_001542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8943615.1"
                     /translation="MNEHDEAEWTKNEEQTEDHEETNTSLEEQTSNPLDDTEEIPMLE
                     ETKIHEKEKVTPTNTNTAENTHAYEITENPGYLEGKEQDHQDIVSLIETAEAELADLR
                     MRKDLIESDFPQLLESYDRFILNEEPISTIGKTSLIEYFTYELLKPLNDIGLERSNSA
                     YDTWKTRHFSINYTLNGQNELVFSFVLPTIDQRYQVESMSLLNVSPETMEINVQDDRV
                     LSLIRHWSVDRVFSAGQITIFNHKLNQLLAHARTLGFTVNQTLLDNTKPLRLNLQSEF
                     ELTNEVLDDIFIVAMKNPSYDMEKINETTYQVRLDKGQSLTITTNEKDQTVLSISSGD
                     YPRSVIDFFINYEFLVPLMVRKS"
     gene            4354..4872
                     /locus_tag="IV334_001543"
     CDS             4354..4872
                     /locus_tag="IV334_001543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8943616.1"
                     /translation="MSTNKIAQLSLLSAACVAGRIAFQFIPNFQPMTAIFLFIVLYLS
                     LKDALIVMSLSIAISSFYFGVGPWVIGQWISYTVVLSLFSIVCLRKTVQRNLWLKGVC
                     FFLAGMVYGIGMTVFDTLLYRLPQPWIYYLQGVSFDLMHSIGNVVFFLVFLPVVKRFY
                     NHSGGKNEKNNL"
     gene            4853..5245
                     /locus_tag="IV334_001544"
     CDS             4853..5245
                     /locus_tag="IV334_001544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4430 domain-containing protein"
                     /protein_id="HAP8943617.1"
                     /translation="MKKIIYSLLAMSVSILILTSCSTTYTQSASNQKEEVKTSQKATI
                     TLQENGQNFSEKEVFFKKGDDLLSILKRNFEVKEDNGFITSLEGHSQDEKNQLYWMFT
                     VDGKSATTGAKEIKLNDGQSVIFNLSKL"
     gene            5321..6256
                     /locus_tag="IV334_001545"
     CDS             5321..6256
                     /locus_tag="IV334_001545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HAP8943618.1"
                     /translation="MENFKKSGMFSVIAALGANILVAISKFVGYAISGSAAMLNESIH
                     SIVDCSNQILLLFGDKQANKGQSELHQFGEGRAKYFFSTIVAMMLFFGGGALGVMEAF
                     EKLTHPSHEVGNPIIVIAILLFGLVIEGSSLRVAMKEIKELNTEKLSTMRFLRESRHS
                     EILIIFTEDLCAVIGLVLALAGTILTYATGIAAFDAISGLLIGFLLMGAAIFLAKEFY
                     SLIIGESVTASDLSKIKLAFERPEISRLIDIKTVHLSPTEILVAAKADIVGNKENEAY
                     SVINDIEKNMRQSLPDKKMYIYIETDKYDPNYSRK"
     gene            6389..7168
                     /locus_tag="IV334_001546"
     CDS             6389..7168
                     /locus_tag="IV334_001546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HAP8943619.1"
                     /translation="MNKKKSAPWLQALHAVMPLCISYIPVGLACGVLLQKVGFDPLLS
                     GLLSILVFSGGAQFLVASMLTTQASFATTLLMVFFLELRYTLLGASLAGFMKKEKRTF
                     LAVFSQSLNDENYAVNYLKFSTDPSWNKRKALYVNWFSMTAWAGSNMIGNFFGSVIRV
                     DADLVHFALTAMFIFMFIMQMKSALLIFTGLFSGVLGVVFMVLFQNTFGLIAATVIAS
                     FAGFMLEKAFKKEKAKKLRDRGKDVNEPLFNFPEQEANHHD"
     gene            7161..7478
                     /locus_tag="IV334_001547"
     CDS             7161..7478
                     /locus_tag="IV334_001547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HAP8943620.1"
                     /translation="MTKLYLLLLVACLFIVAYIPRLFPMLYFTHRKVPSWFSDWMKYV
                     PVALFAALAFKDVFITHEHLDIAWNIKIAAMILVAGVAYKTRSMALSVLTGLASVFLL
                     SML"
     gene            7722..9332
                     /locus_tag="IV334_001548"
     CDS             7722..9332
                     /locus_tag="IV334_001548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HAP8943621.1"
                     /translation="MNKKLMQGTFWLTFANLLCKVLGVVYLIPWLSMMGNNQDGMLAT
                     TLYNVGYLPYGLFLMLGTVGFPNAIAKKVAVATKEGDNAACRLIFRSTINIMFVIGIA
                     SAALMYLFAPLLAGISPIANVNNGILAIRSLCPSLIAIPILSAMRGYFQGKNDLRPYG
                     TSLIIEQAVRVIVILAGTYYLRVLTDGTILEAVLISTVASFFGGLAAIIHMYIVGRRK
                     DFFELKDFLISSRYFERENRSASVSIIRETLPFIFVGSVITIVQMIDQVTMKPLLHFF
                     RPEIADQQLEFLFSRASVNPNKLTLILISMVGTVAISSLPILSTMKKKDRIQIEKTVG
                     DSFSIALLILLPSLTGMSLLAGPLYTLFFGYDPESVGYFQMALLASLFFSLFTILSTM
                     LQSLNHHRFAIRLTVEAIILKVIFQVIGLGLVGGYGMSLSNGLAFGIVFVRGYHFMCK
                     EYRIAPLAKISNFFLKTFRSTLIMLAVCFAVFFLLNQRLSMESKSYAMIYCVAVGSIG
                     GLVFLFSQFGKNGFRMLKNVKHHSRSKE"
     gene            9489..10337
                     /locus_tag="IV334_001549"
     CDS             9489..10337
                     /locus_tag="IV334_001549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HAP8943622.1"
                     /translation="MKLAVVTDSSAYLPERIKNHPDLFVIPIPLIMDGKIYNEGIDIE
                     ADEYYGLLKNSKDFPTTSQPVVGEVLGLYEELKEKGYDTILSIHLSSGISGFVNTLFA
                     MKDDISGVTVIPYDSKITSMPMGHMVEAALDLNDEGQSLDEILAHIDRIRDNTYAYLI
                     VDDLNNLVRGGRLTNGAALIGSLLKIKPILTFSEGKIVLFEKIRSSKKAFARAEEIIG
                     RRNNEIQPPVKLYVIHANNLEVAIAEKEKLQKKYPNAEIEIGHFGPVIGTHLGEKAIG
                     IAISAQ"
     gene            10568..11047
                     /locus_tag="IV334_001550"
     CDS             10568..11047
                     /locus_tag="IV334_001550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8943623.1"
                     /translation="MEIPTNLKHKPVIVVEDYDKVDGRNALNTDAKGLSLGLAQWNDR
                     GKVDISGKVWRHTGEKWSRQSEELPITRILDLAILAAQGSLYFQDAYRYEKFYDPENP
                     VVDIIGLQGNRMTVEVDTKNPKIDEDIILYYDTLQKDGELIGERFRILKRLLDELGY"
     gene            11080..13308
                     /locus_tag="IV334_001551"
     CDS             11080..13308
                     /locus_tag="IV334_001551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA domain-containing protein"
                     /protein_id="HAP8943624.1"
                     /translation="MENFSLTENRTFSRLEKQMIWKKPKSHRTSAEEWRIASEIKGNW
                     QDPEMKISNVLLEGDAGSGKTQLAKALSADLQLPYTKVTCFADMDKSDVFGALLPVVD
                     SNKEQDQELLEAIYQTDTLEAVLTLIQNHYGIDPFSAKEKLAELVQRIEADYPDAIHY
                     SYYPSEIVRAIEKGYLLEIQEPTVIRDASVLVALNSALEPNGMLNLPTGIIRRHPDCV
                     IVITTNRNYQGNRPLNESLRDRMQHAEKMDLPALEVMAERAMAKTMIQKQELLLKMAE
                     IIRLLDETAKANAIKGVAGMRSYFYWANTYKQGQDLLQSIYPKVLYKLSTDSDELAIL
                     EQALSDSGLLLELQDILRKEKWGHPQSEHTKGRTISAEEANERNITENAEEILRLDEK
                     KSLSAEEPNEGEEKQEETIEKNEIPPQMQKERSSDIEGKNQKEQQTGDDSQEMSASDM
                     EAHDKEIKKQLNKEARQIMKETIHEKEGLIVHRPAFQGKTPEARELHMQVMPIVESLS
                     RQILELLDNEQTETYQKGKYEGQRFNASKVAYGDLRNFDKKNPPHEQPSLAVAVRIDE
                     SGSMIRDDRIEAAKKAAIAIAEFSKKVDIPLLIYGDTADRSSREKTSLFSYKEFEDGF
                     QWLNEKLVTMKPRQNNRDGAALRLAAEKLNRQSATTKLLLNISDGQPKALPDYTGKKA
                     KEDIQSVLKEYERQGILFVSAAIGQDKEEIKSIYGESRFVDITDLNEFPKQMIQLIAR
                     YL"
     gene            13437..15080
                     /locus_tag="IV334_001552"
     CDS             13437..15080
                     /locus_tag="IV334_001552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HAP8943625.1"
                     /translation="MIQEQKWWKEAVGYQIYPASFKDSNGDGQGDINGIREKLNYLKD
                     LGIGFIWINPIYQSPFVDNGYDVSDYQDIEERFGTMEDFDLLLKEAHELGIKVIMDLV
                     INHSSDQHQWFEESKKSKESPYRDYYIWVDGVDGKEPNNWTSIFGGSAWEYSHETGQY
                     YLHVFAKEQPDLNWESEKLKEELFNMIRWWLDKGIDGFRLDAISHVKKDEYSVKATEN
                     PFSPFQNVSGIEEHLTDLKHVFEEYDIMTVGEASGVTAEEGPQWVGKDGYFDMIFEFD
                     HIHIWQQEKEGQLDVLKLKLALSAWQTSLDGIGWNALYMENHDVPRAVSVFGDTRPDF
                     WAMSAKAIAMMYFFLQGTPFIYQGQEIGMTNMPFESIDQVDAVDSKRLYKRLLAEGKT
                     REEALDIIRETTRDNSRTPMQWTSEQYAGFSTHEPWLITNPNTKTINVEQQEYEPESV
                     LQFYKNMIRIRQTNKGLIYGSYKEYLHEHPQLYVYERYLEDEQYLIMVNLTESLADYE
                     LPKEADQSWTLLLSNSSSGEFEAKGILAPYEARLYKTNK"
     gene            complement(15153..15698)
                     /locus_tag="IV334_001553"
     CDS             complement(15153..15698)
                     /locus_tag="IV334_001553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01440 family protein"
                     /protein_id="HAP8943626.1"
                     /translation="MKLSEKELKDQLTEIVNDILAEAHLKKGDLFVLGCSTSEVVGGH
                     IGKNSSAEVGQWIIRTLKELLDPKEIALAVQGCEHLNRALVVERTVAEAKNFEIVSVV
                     PALHAGGACSVAAFDQFNDPVEVEHVVGQAGIDIGDTSIGMHIKHVQVPVRPRLKTLG
                     QAHVTALRSRPKYIGGPRANY"
     gene            complement(15721..16206)
                     /locus_tag="IV334_001554"
     CDS             complement(15721..16206)
                     /locus_tag="IV334_001554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HAP8943627.1"
                     /translation="MKTKQLTIYAMLIALTVALSLTILIPVPATNGFVTLCEAGIYTA
                     AFLYGPTGGLVVGASSGLLIDLISGYPQWAVFSLVIHGLQGYVAGSFSKSSTRMWGLG
                     LLLGSLVMIIGYGIAGWILYDWPAGVASIPGNIFQNLIGMGLAFPITASLQRIQARKQ
                     Y"
     gene            complement(16196..17008)
                     /locus_tag="IV334_001555"
     CDS             complement(16196..17008)
                     /locus_tag="IV334_001555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylpyrimidine/phosphomethylpyrimidine
                     kinase"
                     /protein_id="HAP8943628.1"
                     /translation="MKRILTIGGSDPFAGGGIQTDLKTFENFGLFGLSALTSIGALDI
                     NERFMLESISLELLKRQLVSIDKMTSLDGIKIGLLNSSEAIMVVRDFIKTKQNIPIIL
                     DPVLAFKETEAVINQLYISQLIEELFPLVTMITPNLAEAALLSGQALPSSLTEMIHLA
                     KELTASGTKNIVIKGGTRMAGDEAVDLLYSIDHYEVFSHKKIDTTTVNGAGCAFSSAI
                     TANLVLGADFTKAVAESKKFVAHCISNGVLMNDHTGSVWSGGIQKGGMRNEN"
     gene            complement(17147..>18258)
                     /locus_tag="IV334_001556"
     CDS             complement(17147..>18258)
                     /locus_tag="IV334_001556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="acyl-CoA synthetase"
                     /protein_id="HAP8943629.1"
                     /translation="PILYLMEQPALSVSQSFLDKNEISYMTHTSGTTGIPKLICHSAH
                     SMGWRTKWQKTVFTKISEKKLIAFHISPVHSRFNIGISSLMSMGFPMMPLANAQSSKV
                     VHMLEAHRPIALETHPNNFVQWRFTAKEHPEAFSGIRYYHSTFDAINNQTMKTFLRTS
                     MAQDAVFLQVYGQSECGPMILKAHTLESLKTSDARDMGVGLEDMTSARITDSVGNVLP
                     ENTDGHIQLLSKGRALTYYKEDARFQENVYGDWWDSGDYGFMDKHGHLFLKDRQVDLI
                     ETINSTLAIEDFLLDSLDFLAEVVIVRDKNHSPQPIIALAPDKEMDWNRWWEQVHELP
                     RLNVPIIRDFDAIPRTATMKVQRLQIEKELKSQKS"
BASE COUNT         5944 a         3317 c         3646 g         5351 t
ORIGIN      
        1 tcattacttt tttagagtga aacgcattca ttttccatta gaaaataaag cgaacaaaat
       61 aaaaggttta aaaagtgtaa cgtcttagta aagtctataa aaaaacgaaa ctatcgatta
      121 agctaatagt ttcgttttca acatttataa gtaaactttt tctattggta atatagattt
      181 tcggaagggt agacaggtgc gcaggtaata tcgattccta gttttttcaa tgtttgttca
      241 tcatttttat tgaccatcac agtagagtgg gcttgtgctc cctcaagttc agctagttta
      301 tcataagcaa cttgagcgat tggattcgtt actgcactga ttgataaagc gatcaagact
      361 tcattgatgc ttagtgcatc caaagaggaa tgaagtgtct ttgatttcaa tccttgtatt
      421 gtttcaagaa ctaatggtga caataaagga atgtcatctg cgatatgggc taaatgtttt
      481 actgcattta agataactgc cgctgacgca tccatcaatg aacttgtacg cccggtaatg
      541 atgacttcat ctggtaattc gattgcaatc acagcagaag gatcttttgt aggactttgc
      601 gcttgaatct tatctgcata gtcatgggca gggactacca cgtttcggtc ttcaggacgt
      661 aaaccgactt cttccataat cagtttgatt cgattaactg cttcttcgtc gatcaatcct
      721 tttttgaagt cgcattctgt ttggaaataa cgacggatga tttcttgttt agaggcttct
      781 tggacaacgg tatcatccac gatcccaaat cctacacgat tcacacccat gtcagtagga
      841 gattgataaa cagattcttt accagtaatt ttttcaataa tacgtttgat tacaggaaat
      901 gtctcaacat cccgattata gttcacagct actttattgt aggcatcaaa gtggaaggaa
      961 tcgatcatat tgacatcttt tagatcgact gtggctgctt cataagcaat gttcagcgga
     1021 tgtttcaacg gaacattcca tactgggaaa gtttcgaact tggagtaacc agctgcatgt
     1081 cctttttggc tttcatgata cagttggttc aaggaagtag cgagctttcc acttcctggg
     1141 ccaggagctg tcactacaac gattggttta gttgtttcaa tatactcatt ttttccaaaa
     1201 ccatcttctg agacgatttt ttctacgttg actggatagt cttcgatttc tgagtgttta
     1261 tagaccttga tgtttcgtct ttctagttta ttgataaaga ctttcgtggc aggctgtcca
     1321 ctatagcgag tgataacgac actgttgatt aataagccat aaccacgtaa ttcatcgatt
     1381 aaccgcaaga tatccatatc ataagtaatc ccataatcac cacggatctt attacgttca
     1441 atatctccag catagacgca aatcagtatt tcagcttgat ctttcaattt ttgcagcaat
     1501 ttgattttcg catcttcttc aaatcctgga agtacacgtt tcgcatgctt gtcgtcaact
     1561 agcttcccac caaattctaa atataatttg tcatagtggt tcactcgttc caaaatatac
     1621 gctgactgct cttcaatata tttttctgaa tcaaaaccaa tttttttcac taggttccct
     1681 ccttaagtac taaagcattt tttcattttt ttgaggattc gtcaactgga agagcgaaat
     1741 tcctcaaaaa aacaaagaaa atggtttaaa ccaacttgtc tagatagtta ttcattggta
     1801 tgatagtttt tgataaggaa ggtgctacat gaaaaaaaga cagaaaaaga aaaacgcata
     1861 taaacactat atccgttcga tttttaccgg ttatgaaaga atgcttgaag atcccgaatt
     1921 ggaacaattg acgtttactt atctaaacga agaaacgcag ctaactcgtg atgagcatca
     1981 gcgcattcat ttcacaacaa gagatctgcc tagtaaataa aaaataagat aaaggtcatc
     2041 ggattcgatt ctgtcctatc agatggaaag actagttgat cgaaaaaaag gctgtaccaa
     2101 attttggtgc agcctgtttt atggagcatt gcccgctctt ttttataaca attttttaca
     2161 gaaaatggta ctattgaagt attaataagt gaaaaacaga tgagtaagag gaggcaagta
     2221 gtatgaaaga gccagataca aatgcaaatt atgagccaga cgaactagag gagacgaatg
     2281 cggatcagga tacaggacaa gaatcgtctt ctttttacga tatcaaagaa aaagcaagca
     2341 tgaatctttc ccaattatta gaagacttac aggcttttga aaaagctgtc cgcgaagaag
     2401 atatccctga gatctaccgt ttatacaatg gccgattgaa tgaagaatta aaaagaagct
     2461 caaatgaaaa tcacgagatc gatcaattgc tgatgcgtaa gatccatgat cgttttttac
     2521 aggaatttcc ttttatggaa caagtagaaa acatctcgcc aacgatgagc tattacaaaa
     2581 tcggcactta ttaccatgat cggcccacgg tcggtattga tgcaagtctg ccagaaattt
     2641 ttgttttgcc taagatcgat gaagagtggg agaaatattc ccaaccggag tcaacggact
     2701 atgacaaaaa aatcaatgag ctagacgcaa aggtgatcac tgctcagaca gaaattgaac
     2761 gtttagatga acaaataaaa gaaatcaatc gacaaatgac tgatttgaac gacaataaag
     2821 gattcttgaa tcgtaaaaaa atcgaggaag aaattcaaga attagagaag aagaagcaag
     2881 tactatctaa tgaaaaatta ggctggcttc cttatattga gacgcctgaa acaatccagc
     2941 agcaaaaaga agcattgaag caagaagcaa gagcagacca gcttcgtgca gccatcgtag
     3001 aaaaagaaca aaggcaaatc agacgctatt tcggtagcaa agagggattc ggtcaagcaa
     3061 tccatgaatt cttgatgaat tatcttggca atgagaatcc taataatcaa tctaatgaag
     3121 gaggttccga gtatgaatga acacgatgaa gcagaatgga caaaaaatga agaacaaaca
     3181 gaagatcatg aagaaacgaa cacttcattg gaagaacaaa caagtaatcc tctggatgac
     3241 acagaagaaa tccctatgct agaggaaaca aaaatacacg aaaaagaaaa agtaactccg
     3301 acgaatacga atactgcaga aaacacccat gcttacgaaa taactgaaaa tcccgggtat
     3361 ttagaaggaa aagagcaaga tcatcaagat atcgtcagtt tgattgaaac agcggaagca
     3421 gaattagcgg atttacgtat gagaaaagat ttgatcgaat cagattttcc acaattatta
     3481 gaatcatatg atcgttttat attgaatgaa gaaccaatct ccacgatcgg aaagacaagc
     3541 ctgatcgaat acttcacgta tgaattactg aaaccgttga atgatattgg tctggaacgg
     3601 tcaaattcag cttatgacac atggaaaaca agacattttt cgatcaatta tacgttgaat
     3661 ggacaaaacg aattagtctt ttcctttgta ttaccgacca tcgatcaacg ataccaagtg
     3721 gaatcgatga gtctcctaaa cgtatcacca gagacaatgg aaatcaatgt ccaagatgac
     3781 cgggtgcttt ctttgatccg acactggtca gtggatcgag tattctctgc agggcagatc
     3841 acgatattca atcacaaatt gaatcaatta ttagcccatg caagaacact tggttttacc
     3901 gttaatcaga cattactaga caatacgaag ccacttcgtt tgaatcttca gagcgaattt
     3961 gaattgacga atgaagtatt ggatgatatt tttatcgtgg caatgaaaaa tccatcctac
     4021 gatatggaaa agatcaacga aactacttat caagtacgat tagataaagg acagtcttta
     4081 acgataacaa caaacgaaaa agatcaaaca gtcttgtcga tctcatctgg agactaccca
     4141 cgatctgtca ttgatttctt tatcaattac gaattcttgg tcccattaat ggtgagaaag
     4201 tcctgattgg ataagttgga ttaccagatg tcattcagat aattctgttc caaaggactt
     4261 gcggcagtta tccagaataa ttgtcacaag tcttttctgt atgtactaaa tggacgtgaa
     4321 taacttgctg atgaaggagg gatttcatta ggaatgtcca caaataaaat agcacagctt
     4381 agcttattgt ctgctgcttg tgtagcaggt aggatcgctt ttcaatttat tccgaatttc
     4441 cagccaatga ctgctatttt tttatttata gtactttact taagcttaaa agatgcgtta
     4501 attgtcatga gtctttctat tgcgatcagc agtttttatt ttggtgttgg accatgggtc
     4561 atcggccaat ggatcagcta tactgttgtt ctgagtttgt tttcaatcgt ctgcttgaga
     4621 aaaacagtgc aaaggaatct atggttaaag ggagtctgtt tttttctggc gggcatggtt
     4681 tacggaatag gtatgaccgt ttttgatacg ctgttatatc gtttgcctca accttggatc
     4741 tattaccttc aaggggtgtc gtttgatttg atgcacagta taggcaatgt tgtgttcttt
     4801 cttgtttttc tgccagtcgt caaacgtttt tacaatcatt caggaggaaa aaatgaaaaa
     4861 aataatttat agtttgcttg caatgagtgt cagtatactc attttgacaa gttgcagtac
     4921 aacctataca caatcggctt ccaaccaaaa ggaggaagta aaaacatcgc aaaaagcaac
     4981 aatcacgtta caagaaaatg gtcaaaactt ttcggaaaaa gaagtgttct tcaaaaaagg
     5041 cgatgattta ctgagtattt tgaaacgaaa ttttgaagtc aaagaagata acggcttcat
     5101 cacttcgctt gaagggcact cacaggatga gaaaaatcaa ctctattgga tgtttactgt
     5161 ggacgggaaa agtgctacta ctggtgccaa agagatcaaa ttgaatgatg gacaatcggt
     5221 cattttcaat ttgtctaaac tataagtttc ataaaaaact gttaaagggc gaatattgcc
     5281 ataattcagt gagttcagtt gatgaaagaa ggaaaataac atggaaaatt ttaaaaaaag
     5341 cggaatgttt tccgtaattg ctgctctagg ggctaatatt ctagttgcta tcagcaaatt
     5401 tgtcggctat gcaatcagtg gcagtgccgc catgctaaac gaaagcatcc acagtatcgt
     5461 tgattgcagc aatcagattt tgctgttatt cggtgataaa caagccaata aaggacagag
     5521 cgagttgcat caatttggtg aaggacgagc aaaatacttt ttcagtacaa tcgtcgcaat
     5581 gatgcttttc ttcggcggtg gggcacttgg ggtgatggaa gcttttgaaa agttgacgca
     5641 tccatcccat gaagtgggta atccgatcat tgttatagca atactgttat ttggtttggt
     5701 aatcgaagga agttcgttac gcgtggcaat gaaagaaatc aaagaattga atactgaaaa
     5761 actttccacg atgcgctttt tgcgcgaaag tcgacacagt gaaatactga ttatttttac
     5821 ggaagacttg tgtgctgtaa tcggtttggt cttggcttta gcaggaacaa tattgactta
     5881 tgcaaccggg attgctgcat ttgatgcgat cagcggattg ctgatcggtt tcttgctgat
     5941 gggagccgcc attttcctag caaaagaatt ttatagtctg atcatcggtg aaagcgtgac
     6001 agcaagcgat ttatcaaaaa tcaagttagc ttttgaacgt cctgagatca gtcggctgat
     6061 cgatataaaa acagttcatt taagtccaac ggagatttta gttgcagcaa aagcagatat
     6121 cgttggtaac aaagagaatg aagcttactc ggtcatcaat gatattgaaa aaaatatgcg
     6181 tcagtctctt cctgacaaga agatgtatat atacattgaa acagataaat atgacccaaa
     6241 ttattctcgt aaataaaacg tttattcatg aattgaagca aaaatgagaa aaatataatc
     6301 ttgtttttaa ataaaacggc tacaatttag cgaatttatg ttacaatgag tccgctgttt
     6361 atttgttgta attaaaggag atattttaat gaacaaaaag aaaagtgcgc catggcttca
     6421 agctttgcat gcagtgatgc cactttgtat aagctatata ccagtaggat tagcttgcgg
     6481 agtattgttg caaaaagttg gatttgatcc tctcttatca ggactgttat cgattctggt
     6541 cttttccggt ggcgcgcagt ttttagttgc atctatgctt acgacgcaag catcatttgc
     6601 aacgacatta ttgatggttt tctttttaga attacgctat acattattag gagcaagtct
     6661 tgccggtttt atgaaaaaag aaaagcggac gtttttggct gtcttttctc aatcactgaa
     6721 tgatgaaaac tatgcggtga actacttaaa gttttcaact gacccttcct ggaataaaag
     6781 aaaggcttta tatgtcaatt ggttctcaat gactgcatgg gccggtagca atatgatcgg
     6841 taattttttc ggctcggtga ttcgagtaga tgctgacctt gtccattttg ctttgacggc
     6901 catgttcatt ttcatgttta tcatgcagat gaaaagcgca ctactgatct ttacaggttt
     6961 attctcaggc gtattagggg tcgtattcat ggtattattc caaaatactt tcggattgat
     7021 tgcagcaaca gtcattgctt catttgccgg atttatgctg gagaaggcat tcaaaaaaga
     7081 aaaagctaag aaactaagag accgcggtaa agatgtcaat gagccgctct tcaacttccc
     7141 ggaacaggag gccaatcacc atgactaaac tatatttgct acttttagtt gcttgtctgt
     7201 tcatcgtggc atatatcccg cgcttgttcc cgatgcttta ttttactcat cgaaaagtgc
     7261 catcctggtt cagtgactgg atgaaatatg taccggtggc attatttgcg gcgttagctt
     7321 ttaaagatgt tttcattact catgaacatc tggatattgc ttggaatatc aaaatagctg
     7381 caatgattct agtagcaggc gtggcttaca aaacacgttc gatggcgttg tcagttttga
     7441 caggacttgc gtctgtcttt ttgctgtcta tgctttaaac tgttttattt tattaaaaac
     7501 agagattgcg acataagttg ttcaaactga tctatccgaa ctgcatttgc aacagtcaat
     7561 tctggatgca agtttattcg tagggataaa aaagattagt gttggcttat gatgcaagct
     7621 cttttttatt ttaggaatta gagagggatt tgtccttcta tgattttttg ctataatagg
     7681 gaagatgttt attttatgaa agaatagttg agggaattta aatgaataaa aaattgatgc
     7741 aaggaacatt ttggctaacc tttgctaatc ttctatgtaa agttttaggg gtagtctatt
     7801 tgattccctg gctaagtatg atggggaaca atcaagatgg tatgcttgct acgacattgt
     7861 ataatgttgg ctatcttcct tatggattgt tcctgatgct tggtacagtc ggatttccaa
     7921 atgcgattgc aaaaaaagta gctgtagcca caaaagaagg agataacgct gcttgtcggc
     7981 tgatttttag aagtacgatc aatatcatgt ttgtcatcgg tatcgcctct gctgcactga
     8041 tgtatttatt tgctccttta ctagccggaa tcagtccgat tgctaacgtt aataatggga
     8101 tactagctat cagaagttta tgcccatctt tgattgctat cccaattctt agtgcaatga
     8161 gaggatattt ccaaggaaaa aatgatctgc ggccttatgg aacttctttg attatcgaac
     8221 aggctgtgcg agtgatcgtt atcttggctg gtacttatta cttgcgcgta ttgactgacg
     8281 gaaccatatt agaagctgta ttgatcagta cagtcgcttc tttcttcggt gggttagctg
     8341 cgattatcca tatgtatatc gtcggacgca gaaaagactt cttcgaactt aaagattttt
     8401 tgatttccag ccgttatttc gaaagagaaa accgatctgc ctctgtctct atcattcggg
     8461 aaacactacc gtttattttt gtcggctcag tcattactat cgttcaaatg atcgaccaag
     8521 taacgatgaa accactgctt cactttttcc gtccagagat cgcagatcag caactggaat
     8581 tcttattcag ccgtgcttct gtcaatccaa ataaattgac attgatcctg atctccatgg
     8641 taggaacagt agctataagc agcttgccaa tattaagcac gatgaagaaa aaagatcgga
     8701 tacaaatcga aaaaacagta ggagatagtt tttcgattgc gttattgatt cttcttcctt
     8761 cattgactgg aatgtcttta ttagctggac cactatacac attgtttttt ggctatgatc
     8821 ctgaaagtgt cggatatttc caaatggctt tgctagcttc tttgtttttc tctttattca
     8881 cgatcctatc tacgatgctt caatctttga atcaccatcg ttttgcgatc cgattaactg
     8941 tagaagcaat tatcctgaaa gtcatcttcc aagtaatcgg attaggatta gttggcggat
     9001 acgggatgag cttatcaaat ggcttagcat tcggtattgt ctttgttcgc ggctatcact
     9061 ttatgtgcaa agaataccga atagcacctt tagcaaagat cagcaatttt ttcttaaaaa
     9121 ctttccgctc caccctgatt atgcttgcag tatgttttgc cgttttcttt ttgctgaatc
     9181 agcgattgtc aatggaatca aaaagctatg caatgattta ttgtgtggct gtaggaagta
     9241 ttggcggctt agtattttta ttttctcaat tcggaaaaaa tgggtttcga atgttgaaaa
     9301 atgttaaaca tcatagcaga agcaaagaat aggttaacta atcgtttttt gctgtcactt
     9361 gaaaaacggc aactcgtatg attacgcttg tcgtttttct attttccaat tttagtttca
     9421 tatctaatct tacttatgat atagttataa ggtctggaaa aattctttga aacgaggaaa
     9481 ttaaacacat gaaacttgca gtagttacag atagttcggc atatctgccg gaacgtatca
     9541 agaaccatcc cgacttattc gttattccaa tccctttgat tatggatggg aaaatttata
     9601 acgaaggaat cgatatcgaa gcggatgaat attatggact tttaaaaaac agtaaagatt
     9661 ttcctactac ttcacaacct gtcgtagggg aagttttggg tttatatgag gaattgaaag
     9721 agaaagggta tgacacgatt ttaagtatcc atctttcttc agggatttct ggctttgtca
     9781 acacattatt tgcgatgaaa gatgacatca gcggagtaac ggtcattcct tatgactcta
     9841 agatcactag tatgccgatg ggacacatgg tagaagctgc cttagactta aatgatgaag
     9901 gtcagtccct tgatgaaata cttgcacata tcgatcgaat ccgagataat acctacgctt
     9961 atctgattgt agatgatttg aacaatttag tacgaggagg acgtttgaca aatggtgcag
    10021 cgctgattgg cagcttatta aaaatcaaac cgatcctgac attttctgaa gggaaaattg
    10081 ttttattcga aaaaatccgt tcaagtaaaa aagcatttgc tcgagctgaa gaaattattg
    10141 gtagaagaaa taacgaaata caaccgccag tcaaacttta cgtgatacat gcaaataatt
    10201 tagaagtagc gattgcggag aaagagaaat tgcagaaaaa atatccaaat gcagaaatcg
    10261 aaattggtca tttcggacca gtcattggta cacatttagg tgaaaaagcc atcggcattg
    10321 ccatttcagc gcaataattc cttttattca aaaaacatac caaaaatatt ccaacatcat
    10381 cttggacttt ctacatgtta ctaatgagcg tgtaaaagaa cgagatggtg tttttatttc
    10441 tatcaatgaa gctctttttc tttgcaatcc tcacttcaat cctctataat gtcgcatata
    10501 gtttagtaaa tgagtaaact actaatttac ttatttacta aaaataaaag gatgaggagt
    10561 taaaataatg gaaataccaa caaatttaaa acataaacca gtaatcgttg tagaagacta
    10621 tgataaagtt gatggaagaa atgcattaaa tacggatgca aaagggcttt cactgggcct
    10681 tgctcagtgg aatgatcgtg gaaaagtcga tatctcaggc aaggtatggc gacatactgg
    10741 agaaaaatgg tcacgacagt cggaagaatt accaatcaca cgtattttag atttagcgat
    10801 cttagctgca caaggaagtt tatacttcca agatgcttat cgttatgaaa aattttatga
    10861 tccagaaaat ccagtcgtag acatcattgg attacaaggg aaccgaatga cggtagaagt
    10921 agataccaaa aatccaaaga tcgatgaaga catcattttg tattacgata cactacaaaa
    10981 agacggagaa ctgattgggg aacgattccg tattttaaaa cgtcttttag atgagctagg
    11041 atattaatag acagagatga tcaaaagggg aagagaagca tggagaattt ctcactaaca
    11101 gaaaaccgaa ccttttcacg gctagaaaag caaatgatct ggaagaaacc gaaaagccat
    11161 cggacaagtg cagaagaatg gcggatcgcc tccgaaatca aagggaattg gcaagatcct
    11221 gaaatgaaaa tcagtaacgt acttttggaa ggagatgccg ggtcggggaa gacacaatta
    11281 gccaaagcac tatctgctga tctgcaactt ccctatacaa aagtgacctg ttttgcagat
    11341 atggacaaat cagatgtttt cggagcctta ttacctgtag tagattctaa taaggaacaa
    11401 gaccaagaat tactagaagc tatttaccaa acagatacac ttgaagctgt actgacactg
    11461 atccagaacc attacggcat tgatcctttt tctgctaaag aaaaattagc agagctagtt
    11521 caaaggatcg aagctgatta tccagatgca atccattaca gctattaccc ttctgaaatc
    11581 gttcgagcga ttgaaaaagg atatttatta gagattcaag agccgactgt catccgagat
    11641 gcctctgttc ttgtggcact taactcagcg ctcgaaccaa atgggatgtt gaatttaccg
    11701 acaggaatca ttcgtcgcca tccagattgc gtcattgtga tcactacaaa tcgaaattac
    11761 caaggaaatc gcccattaaa tgaatcttta agagatcgga tgcaacatgc ggaaaaaatg
    11821 gatcttcctg ccttagaagt catggcagaa cgtgcgatgg cgaaaactat gatccaaaaa
    11881 caagaacttc tcttaaagat ggcagagatt atccgtttgt tggatgaaac tgcaaaagct
    11941 aacgccatca aaggtgtagc aggaatgcgt tcttattttt attgggcaaa tacctataag
    12001 caaggacaag atttactcca gtcgatttat ccaaaagttt tgtataaatt atccactgat
    12061 tcagatgaat tggcaattct tgaacaagct ttgtctgaca gtggattgct actggaactt
    12121 caggacatat tgagaaaaga aaaatggggt catcctcaat ctgagcatac aaaaggaaga
    12181 accattagcg ccgaagaagc aaatgaacga aacatcactg aaaatgctga agaaattctg
    12241 cgcttagatg aaaagaaatc gttgtctgct gaagaaccaa atgagggaga agagaaacaa
    12301 gaagaaacaa tagaaaaaaa cgagattcct cctcaaatgc aaaaagaacg cagtagcgat
    12361 atcgaaggaa aaaatcaaaa agaacaacaa acaggagatg attctcaaga aatgtctgct
    12421 tctgacatgg aagcacatga taaggaaatc aagaagcaac tgaataaaga agcgcgacaa
    12481 atcatgaagg aaacgattca tgaaaaagaa ggtctcatcg ttcaccgacc agctttccaa
    12541 ggaaaaacgc cggaagcacg tgagctacac atgcaagtaa tgccaatagt agaatcactt
    12601 agccggcaaa ttttagagtt actggataat gaacaaacag agacgtacca aaaaggaaaa
    12661 tacgaaggtc agcggttcaa tgcaagcaaa gtagcatatg gtgatttgag aaattttgac
    12721 aaaaagaacc cgccacatga gcaaccttct ttagcggtag ctgtacggat cgatgaatct
    12781 gggtcaatga tacgggatga tcggatcgaa gcggcaaaaa aagcagcgat tgccattgcc
    12841 gaattttcta agaaagtcga tatcccattg ttgatttacg gagatacagc agaccgttcg
    12901 tcaagagaaa agacttcttt gttttcttat aaagaatttg aagacggatt tcaatggtta
    12961 aatgaaaaat tagtcactat gaaaccaagg caaaacaatc gtgatggtgc tgcacttcgt
    13021 ttagcagcag aaaaattaaa cagacaatcc gcaacaacca aactgttgct taatattagt
    13081 gacggtcagc caaaagcgtt gccggattat acaggcaaaa aagcaaaaga agatatccag
    13141 tctgttttga aagaatatga acgtcaagga atcctatttg tctccgcagc tattggccaa
    13201 gacaaagaag aaatcaagtc gatctacggt gaaagtcgtt ttgtagatat tactgacttg
    13261 aatgaatttc ccaagcaaat gattcaactg attgcccgct atctatgata aaataaagct
    13321 caacgaacgc acttagcaga ttcgttgagc ttttcgtttc tagttaccgg taacaaggat
    13381 acttctaaaa tagaatcggt tataatcaag agggaccgtt aaaggagtga aacgatatga
    13441 tacaagaaca aaaatggtgg aaagaagcag taggctatca aatctatcct gctagtttca
    13501 aagatagcaa tggcgatggt caaggagaca tcaatgggat ccgtgaaaaa cttaattatt
    13561 tgaaagatct tggtatcggc tttatctgga tcaatccgat ctatcaatct ccatttgtcg
    13621 ataatggtta tgacgtaagt gactatcaag atatagaaga gagattcgga acgatggaag
    13681 attttgattt attgctaaaa gaagcacatg aattagggat caaagtgatt atggatcttg
    13741 tcatcaacca tagttcggat cagcatcaat ggtttgaaga atcgaaaaaa tctaaagaaa
    13801 gcccgtatcg cgattattac atttgggtcg acggggtaga tggcaaagaa ccgaataatt
    13861 ggacatcgat tttcggcggt tctgcttggg agtattctca tgaaactgga cagtattatc
    13921 tgcatgtttt tgcaaaagaa caaccagact taaattggga aagcgagaaa ttgaaagaag
    13981 aactattcaa tatgatccga tggtggttgg ataaagggat agatggattt cgtttagatg
    14041 cgatttctca tgtaaaaaaa gatgaatatt cagtaaaagc cacagagaac cctttctcac
    14101 cctttcaaaa tgtctcagga atcgaagaac atttgaccga tttgaaacat gtttttgaag
    14161 agtacgatat catgactgtc ggagaagcaa gtggtgtcac tgcagaagaa ggaccacaat
    14221 gggtagggaa agacggttat ttcgatatga tattcgaatt tgatcatatc catatctggc
    14281 agcaagaaaa agaagggcaa ttagatgtcc ttaaattgaa acttgcatta agcgcttggc
    14341 aaacttcatt ggatggtatc ggatggaacg ctttatatat ggaaaaccat gatgttcctc
    14401 gtgccgtttc agtgtttgga gatactcgtc ccgatttttg ggctatgtct gcaaaagcaa
    14461 tcgctatgat gtatttcttt ttgcaaggta cgccatttat ttatcaagga caggaaatcg
    14521 gaatgacgaa tatgcccttt gaatcgatcg atcaagttga tgcagtagat tcgaaaagac
    14581 tttataaaag gctgcttgcg gaaggtaaga caagagaaga agcattggat atcattcgtg
    14641 aaacaacgag agacaacagc aggacaccca tgcaatggac ttcggaacaa tatgctggtt
    14701 tttctacaca tgaaccttgg ctgatcacta atccaaatac caaaacgatc aatgtagaac
    14761 aacaagaata tgaaccagag tcggtcctgc aattctataa gaacatgatc cgcattagac
    14821 aaaccaataa aggtctgata tacggttctt ataaagaata tcttcatgaa catccacaac
    14881 tttatgtcta tgaaagatat ttagaagatg aacaatatct tattatggta aacttaacag
    14941 aatcacttgc tgactatgaa cttccaaaag aagcagatca gtcctggaca ttactcttgt
    15001 caaattcctc tagcggagaa tttgaagcta aaggcatact agcaccttat gaagctcggt
    15061 tatataaaac aaacaagtaa aacttcctcg ttaaagttca ttagttggac tttgcgagga
    15121 agtttttcag attgctctgt caattactca tttcaataat tcgctctagg accaccgata
    15181 tatttaggac gagagcgaag ggctgtcacg tgagcttgtc caagggtttt caaacgaggt
    15241 ctgacaggta cttggacatg tttgatatgc ataccgattg atgtgtcacc gatatcgata
    15301 ccggcttgtc caactacgtg ttccacttcg acaggatcat tgaactggtc aaaagcagca
    15361 acagaacaag cacctccagc atgtagtgca ggaacaacag agacaatttc gaaatttttt
    15421 gcttctgcca ctgttcgttc tactactaat gctcggttca aatgttcaca accttggaca
    15481 gctagcgcaa tttctttagg atccaacaac tcttttaacg tacggatgat ccattgacca
    15541 acttccgcac tggaattttt tccaatatgt cctccaacga cttcgcttgt gctgcatcct
    15601 aaaacaaaaa gatcaccttt tttcaagtgt gcctcagcca atatgtcatt aactatttca
    15661 gtcaattggt cttttaattc tttttctgac aacttcattt tctaaaacct tctttttcaa
    15721 ttaatattgc ttccgtgctt gaatacgttg aagacttgca gttataggaa aggcaagtcc
    15781 cattccaatc agattttgga agatgtttcc tggtatagaa gccactccag ccggccaatc
    15841 atataaaatc caaccggcga taccatagcc gatgatcata actaagcttc ctaataaaag
    15901 ccctaatccc cacatacgag tagaagattt gctgaaagaa ccagcaacat atccttgtaa
    15961 cccgtggatc actaatgaaa aaactgccca ctgcggatat cctgaaatca aatcgatcaa
    16021 tagaccactg gaagcaccaa caactaatcc acccgttgga ccataaagaa aagcagcggt
    16081 atagatcccg gcctcgcata atgtcacaaa tccattcgta gccggtacag ggatcaaaat
    16141 tgttaatgaa agggcaactg tcaacgcaat cagcatagca tagatcgtta attgcttagt
    16201 tttcatttct cattcctcct ttttgaatac cgccagacca gacacttccg gtatgatcgt
    16261 tcatcagcac gccattagaa atacaatggg caacaaattt tttactttct gctactgctt
    16321 tggtaaaatc tgcccctaac acaagattcg ctgttattgc agaagaaaag gcacaaccgg
    16381 ccccgtttac tgttgtagtg tcgatttttt tatgagaaaa tacctcataa tgatcaattg
    16441 aatagagaag atccacagct tcatctccag ccatccttgt gccgcctttg atcacaatgt
    16501 tttttgttcc tgaagcagtg agttctttag cgagatggat catttctgtt aacgaagagg
    16561 gaagagcttg accagaaagt aaagcagctt ctgccaaatt aggcgtaatc atcgtaacaa
    16621 gtggaaaaag ttcttcgatg agctgcgaaa tatacaattg attgatcaca gcttcagttt
    16681 ctttgaatgc caaaacaggg tctaaaatga ttgggatatt ttgttttgtt tttatgaagt
    16741 ctcgaacaac catgattgct tcacttgaat tcaataaccc aattttgata ccatctaatg
    16801 aagtcatttt atcaatcgaa actaactgtc gtttaagcaa ttccaaagat atagattcca
    16861 gcataaatcg ctcattaata tctagtgccc ctatagaagt caaggcgctt agtccgaata
    16921 gaccaaaatt ctcaaaagtt tttaaatctg tttggattcc tccaccagca aatggatcag
    16981 aaccgccaat tgtcaagatt cttttcataa cattttcctc catgaataag agtataacga
    17041 aattttcaaa aaagaaaaac agccaattgg catattttta tctcatccaa tttaaaaaaa
    17101 caaaaagaaa agaaagacca aaatggatct ttctttcctt aaatagttag gacttttgtg
    17161 attttaattc tttctcgatt tgaagccgtt gcactttcat tgtagctgtt cgcgggattg
    17221 catcaaaatc acggatgata gggacgttca agcgaggaag ttcatggact tgctcccacc
    17281 atcgattcca atccatttct ttatcaggag ctaaagcaat aatcggttgt ggagaatgat
    17341 ttttatccct tacaatcact acttctgcca aaaaatctaa cgaatctaat aaaaagtctt
    17401 caatagctaa tgtactattg atcgtttcga tcaaatccac ttgccgatct ttcaaaaata
    17461 ggtggccatg cttatccata aaaccgtaat ctccgctatc ccaccaatcg ccataaacat
    17521 tttcctggaa ccgtgcatct tccttgtaat aagtcaaagc ccgtccttta gacagtaatt
    17581 gtatatgacc atctgtgttt tcaggtagga cattcccaac tgaatctgta atccgagcgc
    17641 ttgtcatgtc ttccagtcca acgcccatat ctcttgcatc acttgttttc aaactctcta
    17701 gtgtatgagc cttaagaatc attggtccgc attcactttg gccatatact tgaagaaata
    17761 ccgcatcctg tgccatcgac gttcgcaaaa atgtcttcat tgtctggttg tttatagcat
    17821 caaaagtgga gtgatagtat cggatacccg aaaaagcttc tggatgctcc ttagcagtaa
    17881 aacgccactg aacaaagtta ttcggatggg tctctagtgc aattggccgg tgagcttcca
    17941 acatatgaac aacttttgaa ctttgtgcgt ttgctaaagg catcatagga aatcccatgg
    18001 acatcaaaga agagatacca atattgaatc tggaatgcac tggagagatg tgaaaagcga
    18061 ttaatttctt ttccgaaatt tttgtgaaaa ctgttttttg ccatttcgtc cgccatccca
    18121 tagaatgagc agaatgacaa ataagtttag ggataccagt tgtaccagaa gtatgggtca
    18181 tataagaaat ttcatttttg tctaaaaatg attgagaaac agacaaagca ggttgttcca
    18241 ttaaataaag aataggaa
//
LOCUS       DACDKM010000049        17807 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20834_2016
            SAMN12640370-rid9891663.denovo.049, whole genome shotgun sequence.
ACCESSION   DACDKM010000049 DACDKM010000000
VERSION     DACDKM010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640370
            Sequence Read Archive: SRR10040891
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17807)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17807)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742835.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742835.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:04:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,012
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 29 of 75
            Pseudo Genes (incomplete)         :: 33 of 75
            Pseudo Genes (internal stop)      :: 19 of 75
            Pseudo Genes (multiple problems)  :: 5 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17807
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640370-rid9891663.denovo.049"
                     /isolate="E20834_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            203..1048
                     /locus_tag="IV334_001557"
     CDS             203..1048
                     /locus_tag="IV334_001557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAP8943630.1"
                     /translation="MFKLLTTFRAVYETRNFSRAAEVLFLSQPAVSNQIKQLERELNI
                     DLFQRNGRKEMLPTKQADLLYERTLVLLEEWEETQQKLLNEQNEEEVCRIVASHTFAV
                     YLLPQLMKHLIKRFPKVHFEIEITNSHESLEQVAKHESDFGFIEKPLETSGVQRYSLM
                     EDQLVIAGDPKSKLWLVREANSGVFHYTQRYFEEMNIQDKKMIVKSNDVIVALLKEGI
                     GRSILSKRSVPENVPMTPLNFHRYFYFIQRTNLVSDQLLEIAEEILRYNESTETSHSE
                     ENLSW"
     gene            1239..2426
                     /locus_tag="IV334_001558"
     CDS             1239..2426
                     /locus_tag="IV334_001558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HAP8943631.1"
                     /translation="MEKKLKASNDNRIDYGVILPVFLLCLIGLLSLYVALSHDPNNPA
                     VLKGVGMQVVWYLVGAAAIVVIMHLNSKWIWKLTPYLYGLGLVVMGLLLKFYDQNLAA
                     STGSKNWFRFGAFTFQPAELMKIAYILMMALIVTKHNTQVKERTMKSDFWLIGKLLIV
                     TIPVLALIMAQDDFGTMLVFLAIFGGIFLMSGISWRIIAPVVILAVVVGAGTIFLVTT
                     EGGRDLLYKVGFKSYQFARIDSWLDPFHDTSGMSYQPAQGLLAIGTGGLFGKGFNVSN
                     IYVPVRESDMIFTVIGENFGFIGGAFVIFLYFILIYRMIRVCFDTNNEFYAYIASGLI
                     MMLLFHVFENIGANIGLLPLTGIPLPFISQGGSSILGNMIGIGLILSMRYQNEAPARS
                     SRR"
     gene            2432..2788
                     /locus_tag="IV334_001559"
     CDS             2432..2788
                     /locus_tag="IV334_001559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase family protein"
                     /protein_id="HAP8943632.1"
                     /translation="MYTFYWYPKCSTCKKAKAWLDQHGVSYQTVDMIKEVPKASTIET
                     WLETSQVPIRRLFNTSGMKYRELGLKDQVGEFTASKASQVLSTDGMLIKRPLILREDQ
                     LVAIGFNEKIYEGELI"
     gene            2790..3128
                     /locus_tag="IV334_001560"
     CDS             2790..3128
                     /locus_tag="IV334_001560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein H"
                     /protein_id="HAP8943633.1"
                     /translation="MAGKHLKKKDNLWILFNGKEYCVGLTNEAQEELGDITFANLPKA
                     GQSLKKDEPLIEVEAEKAVNEFASPLTGTVSSVNDKIGEDINILNDSDEMNAWILSFK
                     DVDPKEFDEL"
     gene            3523..4557
                     /locus_tag="IV334_001561"
     CDS             3523..4557
                     /locus_tag="IV334_001561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAP8943634.1"
                     /translation="MSLIQLTNVKKTFNGKNGNIQAVNDVSLNVEKGDIYGIVGYSGA
                     GKSTLVRLLNGLELPTSGEVIVNDQDITKLKNKELRTFRKKIGMIFQHFNLLWSRTVL
                     ENIQLPLELAGVPKSKRKERAEELLRLVGLEGRGQAYPSQLSGGQKQRVGIARALAND
                     PEILLCDEATSALDPQTTEEVLDLLLAINKKLNLTIVLITHEMNVIRKICNKVAVMEL
                     GKVVEEGDVLTVFRQPKKEVTKRFVQQDIEPEADSTELLAELIKENPSGRLATLTFSE
                     RNANEPVISQAIRRYNIDVNVVYGKIKQVKEGSFGSLTVLMTGETKELDQAEVFIKEQ
                     GVGIEVIHRG"
     gene            4550..5236
                     /locus_tag="IV334_001562"
     CDS             4550..5236
                     /locus_tag="IV334_001562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP8943635.1"
                     /translation="MDKSFSETYLDFKQINPETMNQAILDTLYMTLISMVLVTIIGLV
                     LGLILYSIGRKNKPYARIIYGIVSIISNIFRSTPFMILMVLIIPFTKAIVGTMLGAQA
                     AIPALVLSAAPFYARLVEIAFREVSPGVLEAADAMGASYWEIIWKVLIPESIPALISG
                     LTVTTISMIGFTAMAGAIGAGGLGGLAWQEGYQRGNLTVTFVATLIILLIVFIVQGIG
                     DFLTKKTDKR"
     gene            5260..6108
                     /locus_tag="IV334_001563"
     CDS             5260..6108
                     /locus_tag="IV334_001563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAP8943636.1"
                     /translation="MKKKIFGFAAALLLTVGLAACGNNSDSKDSANKADDTTLKVGAS
                     PTPHAEILEHVKPLLKDEGIDLEIVKFDDYVLPNQSLEEGDIDANYFQHIPYLNKEIK
                     EKGYDFVNAGAVHIEPMGLYSKKVKDVSELKDGATIITSNSESDWGRIITILQDAGLV
                     KVKDGVDLETATFDDIEENPKNLKFNHTIDPSLLASTYQNDEGDLVAINANFAYGAGL
                     NPLEDAVLLEKDNSPYVNIIATRKGDEDSDKIKKLIEVLHRKEVQEWITDKWGGSVKP
                     VKADAK"
     gene            6305..7078
                     /gene="sufC"
                     /locus_tag="IV334_001564"
     CDS             6305..7078
                     /gene="sufC"
                     /locus_tag="IV334_001564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HAP8943637.1"
                     /translation="MSVLEIKNLHVSIEEKEILKGVNLTMKTGEIHAIMGPNGTGKST
                     LSAAIMGNPNYEVTEGEILFDGKNVLDLEVDERARLGLFLAMQYPSEIPGITNAEFMR
                     AAINAKRDENDKISVMAFLKKLDEKMALLNMPEEMAERYLNEGFSGGEKKRNEILQLL
                     MLEPTFAILDEIDSGLDIDALKVVAKGVNEMRGDNFGALIITHYQRLLNYITPDVVHI
                     MMEGRVVMTGNADLAKRLEAEGYAGISKELGIDYKEEEA"
     gene            7091..8377
                     /gene="sufD"
                     /locus_tag="IV334_001565"
     CDS             7091..8377
                     /gene="sufD"
                     /locus_tag="IV334_001565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HAP8943638.1"
                     /translation="MKEKNWLDYLDAVNDFSLSKGEPDWMRTFRQDALAKADELPLPH
                     IDRVKFHRWSLFDVKETQTISETGTIPAFDAMKDNPVLVQQGSWTIFEQLPVELAEKG
                     VIFTDLFTAMIEYPELVQEYYMKKAVNMNEDQLTALHVAFMNSGIFLYVPKNVVIDEP
                     LESLFIQDGASDEHFFKHVLIVADEHSEFSYLERFQTTKEQVAKSSGNIIVEVIAKAG
                     SKIKYSAVDQLGENITSYMNRRGHILRDASVDWAIGVMNDGHVIADFDSDLAGEGAHA
                     EVKIVAISSGRQIQGIDTRVTNKAPHTIGHILQHGVIREKGTLTFNGIGHILKGAKGA
                     DAQQESRVLMLSDKARGDANPILLIDENEVTAGHAASVGRVDPEEMYYLMSRGLHKEE
                     AERLVIRGFLGSVLTAIPVEQVRKELVEVIEGKLNG"
     gene            8374..9609
                     /locus_tag="IV334_001566"
     CDS             8374..9609
                     /locus_tag="IV334_001566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HAP8943639.1"
                     /translation="MIDAKKLRNDFPILFQDVNDEPLVYLDNAATTQKPKQVLQALAY
                     YYEHDNANVHRGVHTLAERATHDYEQAREKVRAFIHAKETAEVLFTRGTTTSLNWVAR
                     TFGDRYIKAGDEIVLSYMEHHSNVIPWQQLAKRTGAVLKYIELTEEGFLDMEDAAKKI
                     TDKTKIVSIAHVSNVLGVFNPVQELAELVHENGGVIVVDGAQSAPHMAIDVQALDCDF
                     FAFSGHKMCGPTGIGVLYGKRKWLEEMEPVEFGGEMIDFVELYDSTWKELPWKFEAGT
                     PNIAGAIALGHAIDYLQKLGMDNIHRYEEELAAYVLPKLQAIDGLTVYGPQDPNFRTG
                     VVAFNLDGLHPHDVATALDMEGVAVRAGHHCAQPLLKYLEVSATARASFYFYNTTQDA
                     DRLVEAIMATKEFFQHGTI"
     gene            9596..10066
                     /locus_tag="IV334_001567"
     CDS             9596..10066
                     /locus_tag="IV334_001567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUF system NifU family Fe-S cluster assembly
                     protein"
                     /protein_id="HAP8943640.1"
                     /translation="MGLSKLDNLYRQVILDHSSHPHHRGSLTDSTSKIELNNPTCGDV
                     IELQVDVKDGIIKNIAFDGSGCSISTASASMMTDAVIGKSIEEAEHLTVDFLQLVQGK
                     EVKDPDQLGDAAMLGGVAKFPARIKCATLAWKALGKAMENDGTAAFHHYHKEEE"
     gene            10071..11462
                     /gene="sufB"
                     /locus_tag="IV334_001568"
     CDS             10071..11462
                     /gene="sufB"
                     /locus_tag="IV334_001568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HAP8943641.1"
                     /translation="MGVPELEEYKFGFHDDVEPVFSTGEGLTEEVIREMSRIKGEPEW
                     MLEFRLKSLETFNKMPMQTWGPDLSDIDFDAIKYYQKPSDKPARDWEDVPEKIKDTFE
                     KIGIPEAERAYLAGASAQYESEVVYHNMKDEFEKLGIVFTDTDSALKEYPELFKEYFS
                     KLVPPTDNKLAALNSAVWSGGTFIYVPKGVKVDVPLQTYFRINAENTGQFERTLIIVD
                     EGASIHYVEGCTAPTYSSNSLHAAIVEIFTRKDAYCRYTTIQNWSDNVYNLVTKRAKA
                     YEGATVEWIDGNLGAKTTMKYPSVYLDGKGARGTMLSIAFAGANQIQDTGAKMIHNAP
                     NTSSSIVSKSISKDGGEVNYRGQVTFAKNSAGSISHIECDTIIMDEWSKSDTIPFNEI
                     HNSQVSLEHEAKVSKISEEQLYYLMSRGLTESEATEMIVMGFVEPFTKELPMEYAVEL
                     NRLISYEMEGSVG"
     gene            complement(11557..12699)
                     /locus_tag="IV334_001569"
     CDS             complement(11557..12699)
                     /locus_tag="IV334_001569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAP8943642.1"
                     /translation="MAKISKYKKKNGQVAWMYKGHIATDPRTGEKINTTRRGFSSKQE
                     AQKDYDEYRHQILYGVKKKAPDMTFEDLYNEWITHQRTSVKASTIAISVRYANNQILP
                     AFGKLKLSNISVPYCQKVVDEWHSKYESYDYMRKQTAQILRYGVAMQYIDNNPMEKTL
                     LPRKKEYEKNRKFYSKEELNNLLDAFKDFGNMKQYAFFRLLAYTGMRKSEVLALQWKD
                     IDTFNKELHVNKTLAVDEFGKVIIQSPKTRASRRVISLDTETLSILNNWKLQQKEEYL
                     KLGYNTSSKEQHVFTTVKNTLYIPNTVNDWLRYILKKYNLPRITPHGFRHTHASLLLE
                     AGESVKVVQQRLGHENSKVTLDIYAHITNNAPKKTGQDFADMMAHQ"
     gene            complement(12738..13472)
                     /locus_tag="IV334_001570"
     CDS             complement(12738..13472)
                     /locus_tag="IV334_001570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP8943643.1"
                     /translation="MIKRPPINYLERKKILGTKIKAIRKSKKLTQPAFGLMINNGQLI
                     DKKTIYEWEKGTYLPIPERLSRIADLGNMSIEELVCGNVEEYILGIILYRDSIVLDGI
                     TFPDKNLFQHLRQQFPPVHSNLDTWLDRYSKLEPEMQEFIANKTCNKVKNEKISLFNI
                     LKIEELFINAIVEEFDNNILFLTSSIEELLERMVDEWLPIQLKDMSYPEEAVREITDN
                     INKLEQTISSIGKKYTKKKMKGGDTI"
     gene            13704..13979
                     /locus_tag="IV334_001571"
     CDS             13704..13979
                     /locus_tag="IV334_001571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP8943644.1"
                     /translation="MSLQIIDSIVSIQVEQQVKEIITVLKPMLEDIISGVTLEYMDYQ
                     QASEYLGVSVGTIRKYVSQYGLPVIKIDTVIRFKKSDIDSFMERYKG"
     gene            14077..16074
                     /locus_tag="IV334_001572"
     CDS             14077..16074
                     /locus_tag="IV334_001572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8943645.1"
                     /translation="METSMINIPIYVDQEKLLEKPVTKKEKTDVRDRCLDNYREVTRD
                     EFVEILNSSQSFIPSKTKNKGNDKESFVETRVIILDVDNTVKDEKGKVVDLSKDDSRY
                     LSVEKVLSINLVHNSAFAIQKSIRYSENLEKYKIVFLLKEAITDYGKMVAVYEYLKKN
                     IPGCDDNVNASNRMFFGGYKSDAVIIINEDNMLDITELPIDFTMTCSKNCQSDSGREY
                     ELVNETDFVKLIKNDDKEEMKQWFKDCLFDSSDMSFNEIYERLLTIDMNKLLKSSKNL
                     RCLFHNDHNPSASIFTSKTGHSIFYCHSSNCDISENFIGVVMLILKKDSRVETFNWLL
                     ENLDLYPTHFRKLKEESKVLFDTLESMKDKLFRTIRNYLEEMRRVYDVLLSDVNFFDD
                     SKESVTCILSGEQLAKKMSSYYGKTYDIDKCNKLLSLMTFIGLIKKLDDEDVPLKMLE
                     YLNRLKEEKALGNFNHKRSNVYRLDVFNATSVIQKLNEDILPLLEKIHFTYQYFSYEW
                     VKICFNEKEAKRVFPQNSRSELSKEKQLILDEAHEVLQGLSWQGTYLMTEKELINTVC
                     ERLRNVGDNSVKNALIKNRGYFVKQGFTLERASKDIKKLYNVKASSSFLIYLAKGAGI
                     DETIKELILSGAGIVPLYQFTSVTRDDGRRVRKRKDPIVLN"
     gene            complement(16150..16485)
                     /locus_tag="IV334_001573"
     CDS             complement(16150..16485)
                     /locus_tag="IV334_001573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP8943646.1"
                     /translation="MKQKQYAKVIREKRKALGWTQKELAEKIFSTQQAVARWENSVTE
                     PNLDSLTALSRALGTPVSHFLDNVVVDYEEEFLALYRSLSTEDAVRTIDYMKLLKRQE
                     NERNQLLKD"
     gene            16612..16827
                     /locus_tag="IV334_001574"
     CDS             16612..16827
                     /locus_tag="IV334_001574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP8943647.1"
                     /translation="MKKVSNLSRYRKEKGLSQTELAKKMNVTQQCVSSWQTGRTIPKP
                     YQMKMLSEILSVPMNELFSDVFNKVNS"
     gene            16864..17226
                     /locus_tag="IV334_001575"
     CDS             16864..17226
                     /locus_tag="IV334_001575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010717431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HAP8943648.1"
                     /translation="MYCRKCGKEITDESKYCQFCGALQETDETTGTDTVKKSTEQTIN
                     EMKEQIVHGQETGKLYLIIGWISMVVSLAFIPVVFGAVAVIMGYLYREKDEKMGTILM
                     IAGVAGAIFGVLLGMSMY"
     gene            17272..17598
                     /locus_tag="IV334_001576"
     CDS             17272..17598
                     /locus_tag="IV334_001576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8943649.1"
                     /translation="MNKVNMNKKFVIIGIVVLAIIGIIFSKMTGTSGLEKSSKPLVTQ
                     ILQEQYNLNRSCDDVTITHENGDNTYRAKAILDNGSAININIEYYPKKDRIYVEIPYA
                     EVLMLN"
BASE COUNT         5859 a         3075 c         3733 g         5140 t
ORIGIN      
        1 ttagtaccaa gatgaacgaa tacattgtaa aatgaatagg aatgtatgat tacgagcaag
       61 cagacagctg attgtcttca tctaagttac tagttggatc agcaactgtt ttgttgtaca
      121 tttctattat agatggtttt ttgtatattt aaatcaaata gtttttatag taattatcat
      181 aaaaagttat ggaagtggtg ccatgtttaa gttattaacg acttttcgag cagtatatga
      241 aacaagaaat ttttccagag cagcagaagt actgttttta tctcaaccag cagtctcgaa
      301 tcaaatcaaa cagttggagc gggaattgaa tatcgatctt tttcaacgta acggaagaaa
      361 agagatgctt ccaactaagc aagccgactt attatatgaa cggacactgg ttttgttaga
      421 agaatgggaa gaaactcagc aaaaattgct caatgaacaa aatgaagaag aagtatgtcg
      481 gatcgttgct tcccacacct ttgctgtcta tcttttgcct cagttgatga aacatctgat
      541 caaaaggttt cctaaagtgc attttgagat cgaaatcacg aattctcatg aatcattaga
      601 acaagtagcg aaacatgaat ccgattttgg ttttatcgaa aagccattag agacttccgg
      661 cgtacaaaga tactctttga tggaagacca gctagtcatc gctggggacc caaaaagcaa
      721 attgtggctt gtaagagaag caaactctgg tgtttttcat tacacgcagc gttattttga
      781 agagatgaat atccaagata agaagatgat cgtgaaaagt aacgatgtaa ttgttgcgtt
      841 gctgaaggaa ggaatcggtc gctcgatttt gtctaagcgc tctgttccag aaaatgtgcc
      901 aatgactccg ttgaatttcc atcgatattt ttatttcatc caacgaacca atctggtttc
      961 tgatcagctg ctagagattg ccgaagagat ccttcgatac aatgaaagca cagaaacttc
     1021 tcacagcgag gagaatctca gctggtagat aattgcatgt atcaaaaaaa gttctatgat
     1081 acactaaaga taagcagtag gcttttgtac tgcatttgct caagcaactg cacaaacgga
     1141 aagttaattt ttcccaaatg tctatggaaa gactttccat tatctaactg gaccgctata
     1201 ataatcagca gtatggtttt taagaaaagg gatgaaacat ggaaaaaaaa ttaaaagctt
     1261 cgaacgataa tcgtattgat tatggcgtga ttttaccagt ctttctgtta tgtttgattg
     1321 gtctgctctc attatatgta gcacttagcc atgatccgaa caatccagca gtcttgaaag
     1381 gcgtagggat gcaagttgtc tggtatttag ttggagcggc agcaatcgtt gtcatcatgc
     1441 atttgaattc caaatggatc tggaaactga ctccttacct ttatggtttg gggttagtgg
     1501 taatgggact cttgctcaaa ttctatgatc agaatttagc tgcttcaaca gggtctaaaa
     1561 actggtttcg attcggtgcg tttactttcc agccagcaga gttgatgaag attgcgtaca
     1621 tcttgatgat ggcactgatc gtaactaaac ataatacgca agtaaaagaa cggacgatga
     1681 aatcggactt ttggctgatt gggaagctct tgatcgtgac aataccggtt ttagcattga
     1741 tcatggccca agatgacttt ggaacgatgc ttgtctttct tgcgatcttt ggcgggattt
     1801 tcctgatgtc tggaatctct tggcgcatca tcgcacctgt agtaatcttg gcagtagtcg
     1861 ttggagcagg aacgatcttc ttggttacga ctgaaggcgg aagagacttg ctttataaag
     1921 ttggattcaa atcttatcag tttgcacgta ttgattcatg gctggaccct ttccatgata
     1981 cgtcaggaat gagttatcag ccggcccaag gacttttggc aattggtacc ggtggcttgt
     2041 ttggtaaagg attcaacgta agcaatatct atgtgcctgt tcgtgagtcc gatatgattt
     2101 ttaccgttat cggtgaaaac ttcggcttta tcggtggtgc attcgtcatt ttcttgtatt
     2161 ttattttaat ctatcgtatg attcgggtgt gtttcgatac gaataatgaa ttttacgcct
     2221 atatcgcaag cggtttgatc atgatgctgc tgttccatgt ctttgagaat attggagcaa
     2281 atatcggatt acttccttta acagggatcc cattgccttt cattagtcag ggaggatcct
     2341 cgattttagg gaacatgatc ggtatcgggt tgatattatc gatgagatac caaaatgaag
     2401 cacctgcaag atcttcaagg aggtaagaaa attgtacacc ttttactggt acccgaaatg
     2461 ttcaacatgc aaaaaagcca aagcgtggtt agaccagcat ggcgttagtt atcaaaccgt
     2521 tgatatgatc aaagaagtac cgaaggcctc aacgattgag acttggctag aaacgagtca
     2581 agtaccaatc cgtcgcctgt tcaacacaag cgggatgaaa tatcgggaac ttggactgaa
     2641 agatcaagta ggcgagttta cggcttcaaa agcgagccaa gtcttatcga cagatggtat
     2701 gctgatcaag cgaccactga tcttacgaga ggaccagctt gttgcaattg gattcaatga
     2761 aaaaatttat gaaggagaac tgatataaaa tggcaggcaa gcatttgaag aaaaaggaca
     2821 acctttggat cttatttaat ggaaaagaat actgtgttgg tttgacaaat gaagcgcaag
     2881 aagaacttgg agacatcacg tttgccaatc tgccaaaagc aggacaatcg ctaaaaaaag
     2941 atgaacctct gatcgaagtt gaagcggaaa aagcggtcaa tgaatttgcc agcccgctaa
     3001 cgggtacagt ttcttctgtt aatgataaaa tcggtgaaga tatcaatatc ttgaatgatt
     3061 cagatgaaat gaatgcgtgg atcttaagtt tcaaagatgt tgatccaaaa gaatttgacg
     3121 aattataaaa tcagaaaatc acttgaaaaa agttgacact gattcttcca gttgctataa
     3181 tcataagcaa tcctatatga aacgagaaag ctttgaaaag aagagtacat gtcacggacg
     3241 tttgaagaga gccctgtttg ctgaaaatgg gtaacgaaaa tgagatggaa gatggtctta
     3301 gagcagagta agcgagcgta agcaaactta ctacgggagt gcccgttaca gcgcgacagt
     3361 atacggaaag aagctaagtc caagtactgg aagaggctgt tattgcgaag taacggtaaa
     3421 tcaaggtggt aacacggaag ttcaagcttt cgtccttttg cttaaggcaa gagggcgaag
     3481 gcttttttgt ttgaaaaatc ataagagaaa gatgtgaatg gcatgtcatt aatccaacta
     3541 acgaatgtca aaaagacatt caatgggaaa aacggaaaca tccaagccgt caatgacgtt
     3601 tcactaaacg ttgaaaaagg ggatatttat ggaatcgtcg gctattctgg cgcaggaaaa
     3661 agtacattag tccgtctcct gaatggactt gagcttccca cgagcggcga agtgatcgta
     3721 aacgatcaag atattacaaa attaaaaaac aaagaattac gtacattccg aaagaaaatc
     3781 ggtatgatct tccagcattt caacctgtta tggtcaagaa ctgtacttga aaatatccag
     3841 ctgccgctgg aattagctgg cgtaccaaag agcaaacgta aagaacgggc agaagaactg
     3901 ttgcgtttag tcgggttaga aggacgcgga caagcatatc cttcacaact ttccggcgga
     3961 cagaaacaac gcgtcggtat cgcacgagca ttggcaaatg atcctgagat cctgctttgt
     4021 gatgaggcta ctagcgcatt ggacccgcaa acaacagaag aagtattaga cttattgtta
     4081 gcgatcaata agaaattgaa tttgaccatc gtcttgatca cacatgaaat gaatgtgatc
     4141 cgcaaaatct gcaacaaagt cgctgtgatg gagttgggga aagtcgtaga agaaggagac
     4201 gtattgactg tcttcagaca accgaaaaaa gaagtaacca aacgctttgt acaacaagac
     4261 atcgaaccag aagctgattc gacagaacta ttagctgaac tcatcaaaga aaatccaagc
     4321 ggccgtttgg caactttgac ctttagcgaa agaaatgcca acgagccagt catttcccaa
     4381 gcgattcgac gctataacat cgatgtcaac gttgtgtatg gaaagatcaa acaagtgaaa
     4441 gaaggttcct ttggttcatt gactgtcttg atgactggtg aaaccaaaga attagatcaa
     4501 gcagaagtgt tcatcaaaga acaaggagta ggaattgagg tgatccatcg tggataaaag
     4561 cttttcagaa acatatctag attttaaaca aatcaacccg gaaacgatga atcaggctat
     4621 ccttgataca ctatatatga cattaatctc aatggtctta gtgacgatca tcggattagt
     4681 tttgggattg atcctttact ctattggacg caaaaacaaa ccgtatgctc gaatcattta
     4741 tggaatcgta tcgatcatca gtaatatttt ccggtcaacg ccatttatga tcctgatggt
     4801 tttgatcatt ccatttacta aagcaatcgt aggcactatg ctaggtgcgc aagcagccat
     4861 tccagctctt gttttatccg cagcaccatt ttatgcccgt ctagtagaga ttgcttttcg
     4921 tgaagtcagc cctggtgtac tggaagcagc agatgcaatg ggagcaagtt actgggaaat
     4981 catctggaag gtcttgatac ctgaaagtat tcccgcattg atttctggat taacagttac
     5041 aacgatctcg atgatcggat ttactgcgat ggccggcgcg atcggcgcag gtggacttgg
     5101 aggtcttgct tggcaggaag gctaccagcg tggtaatcta acagtgacat tcgttgcgac
     5161 tttaattatt ttactaatcg tatttatcgt tcaaggaatc ggtgatttct taactaagaa
     5221 aacagacaaa agataatagg atataggggg atgtaggata tgaaaaagaa aatttttgga
     5281 tttgcagcag cgcttttgct gactgtagga ttagcagcat gcggtaacaa cagcgacagc
     5341 aaagattcag caaataaagc agacgacacg acattgaaag tcggcgcttc accgacaccg
     5401 cacgcagaga ttttggaaca tgtgaaacca ttattgaaag atgaaggaat cgatcttgaa
     5461 atcgtcaaat tcgatgacta tgtattaccg aaccaatctt tggaagaagg cgacattgat
     5521 gccaattact tccagcatat tccttatttg aacaaagaaa tcaaagaaaa aggatatgac
     5581 tttgtcaatg ccggtgctgt ccacatcgaa ccaatgggct tgtactctaa aaaagtcaaa
     5641 gatgtttccg aattaaaaga tggtgcaaca atcattactt caaattctga atcagactgg
     5701 ggacgcatca tcacaattct acaagatgct ggcttagtca aagtcaaaga tggagtagat
     5761 ctggaaacag caacattcga tgatattgaa gaaaacccta agaacttgaa gttcaaccat
     5821 acgatcgacc catcactttt agcttctacg taccaaaatg atgaaggtga cctagttgca
     5881 atcaacgcaa acttcgctta cggcgcaggg ttgaatccat tagaagatgc cgtattgctt
     5941 gaaaaggaca actctcctta cgtgaatatt attgcgactc gtaaaggtga tgaagacagt
     6001 gacaaaatca aaaaactgat tgaagtgctt caccgcaaag aagtccaaga atggatcaca
     6061 gacaaatggg gcggctctgt taaaccagta aaggctgatg caaaataagg tatggaacaa
     6121 aaaagatgag aaataagtct gtaatggctt atttctcatc ttttttatac ctattctaat
     6181 ttagaaatca aaattgttta ataataaata aaagatattt gattttagta aggaaggtca
     6241 ttgcgcttca ttctcaatta gtctaaaatg agtgagaacg aaagagaatt tgggggtata
     6301 atgcatgtct gtcttagaaa taaaaaatct acatgtgtcg atcgaggaaa aagaaatcct
     6361 taaaggcgta aacctaacaa tgaaaaccgg agaaatccat gccatcatgg gacctaacgg
     6421 cacaggaaaa tctaccttat ctgccgctat catgggcaat ccaaactatg aagtaacaga
     6481 aggcgaaatt ttgtttgacg ggaaaaatgt tttagacttg gaagtagatg aacgtgcgcg
     6541 acttggctta tttttagcta tgcaatatcc aagtgagata ccaggtatca cgaatgcgga
     6601 attcatgcgt gcagcgatca atgcgaaacg tgatgaaaac gacaaaattt ctgttatggc
     6661 atttttgaaa aagttagatg aaaaaatggc tttactaaat atgccagaag aaatggctga
     6721 acgttatctg aacgaaggtt tttctggcgg agaaaagaaa cggaatgaga tcctgcaatt
     6781 gttgatgtta gaacctacat ttgcaatttt agacgaaatc gattcaggtc tggatatcga
     6841 tgctttgaaa gtcgttgcta aaggagtaaa tgaaatgcgc ggagacaatt tcggtgcatt
     6901 gatcatcacc cactatcaac gattattgaa ctatatcaca cctgatgtcg ttcacatcat
     6961 gatggaaggc cgagtagtca tgacagggaa tgctgaccta gctaaacgtc tggaagcaga
     7021 aggctacgct ggtatcagta aagaattagg aatcgactat aaagaagaag aagcgtaagg
     7081 gggagcaaga atgaaagaaa aaaactggct cgattatctt gacgcggtaa atgatttttc
     7141 tttaagtaaa ggcgaacctg attggatgag aaccttccgt caagacgcac tagcgaaagc
     7201 agatgaactg ccattgccgc atatcgatcg ggtgaaattc catcgctggt cgttatttga
     7261 tgtgaaagaa acacaaacca tcagtgaaac aggcacgatt ccggcatttg atgcaatgaa
     7321 agacaatcct gtgcttgtcc aacaaggttc atggacgatt tttgaacaac tgcctgtgga
     7381 attagcagaa aaaggagtca tttttaccga cttgttcact gcaatgatcg aatatccaga
     7441 actagtccaa gaatattata tgaaaaaagc agtaaacatg aacgaagacc aattgactgc
     7501 actccatgta gcttttatga acagcggtat tttcctgtat gtgcctaaaa atgtcgtgat
     7561 cgatgaacca ctcgagtcct tattcattca agatggcgca tccgatgagc acttcttcaa
     7621 gcatgtgttg atcgtggcag atgaacatag tgaattttct tatctggaac gtttccagac
     7681 aaccaaagaa caagtagcaa aatcatcagg taatatcatc gtggaagtca tcgctaaagc
     7741 tggttcaaaa atcaaatatt cagccgtaga tcaattagga gaaaatatta cttcttatat
     7801 gaatcgccgt gggcacattt tacgagatgc ttctgtcgac tgggcaattg gtgtcatgaa
     7861 tgacggtcat gtgatcgctg actttgactc agatttagca ggagaaggtg cacacgcaga
     7921 agtcaaaatc gtggcaatca gctcgggcag acaaatccaa ggaatcgata cacgagtaac
     7981 caataaagcc cctcatacga tcgggcatat tttgcaacac ggagtgatcc gagaaaaagg
     8041 aacattgacc tttaatggta tcggccacat cttgaaaggg gcaaaaggtg cagatgccca
     8101 acaagaaagt cgtgtcttga tgctttctga taaagcacgt ggtgatgcga atcctattct
     8161 tttgatcgat gaaaacgaag taactgctgg acatgctgcc agtgtaggac gagttgatcc
     8221 agaagaaatg tactatctga tgagtagagg attgcataaa gaagaagcag agcgcttagt
     8281 gattcgagga ttcctcggtt ctgtcttaac agcaatccct gtagaacaag ttcggaaaga
     8341 attagtcgaa gtaatcgaag ggaagttgaa tggatgatcg atgctaaaaa gctacgcaat
     8401 gattttccta tcttatttca agacgtcaat gatgaacctt tagtttatct ggataatgca
     8461 gcgacgaccc aaaagccaaa acaagtactc caggcattag cgtactatta cgaacacgat
     8521 aacgccaatg ttcatcgcgg tgtccatacg ttagcagaac gcgcgaccca tgattatgaa
     8581 caagcaagag aaaaagtacg ggcgttcatc catgcaaaag aaacagcaga agtgctgttt
     8641 actcgtggta cgacgacaag cttgaattgg gtagccagaa ccttcggcga ccgctatatc
     8701 aaagcaggag acgagatagt gctctcttac atggaacatc attctaatgt tatcccgtgg
     8761 cagcagttgg caaaacgaac aggtgctgtc ttgaaatata tcgaactaac agaagaaggc
     8821 ttcctggata tggaggatgc agcgaaaaag attacggaca aaaccaaaat cgtctccatt
     8881 gctcatgtat ccaatgtctt aggtgtcttc aatcctgttc aagaattagc tgaacttgtc
     8941 catgaaaacg gcggggtcat cgtagtagat ggtgctcaat ctgccccgca catggccatt
     9001 gatgtccaag cactagactg tgatttcttt gctttcagcg gacacaaaat gtgcggacca
     9061 acaggtattg gtgttttata tggtaaacga aaatggttag aagaaatgga acctgttgaa
     9121 tttggtggcg aaatgatcga ttttgtcgaa ttatacgaca gtacatggaa agaactacct
     9181 tggaaattcg aagcagggac accaaatatc gccggcgcta tcgcattagg ccatgcaatc
     9241 gattatttgc aaaaactcgg tatggacaat atccatcgat atgaagaaga actagccgct
     9301 tatgtgttgc caaaattaca agcaattgat ggattgactg tttatggtcc acaagacccg
     9361 aattttcgga caggagtcgt tgcattcaat ttggatgggt tacatcccca tgatgtcgcg
     9421 acagctttag acatggaagg ggtagccgtc agagcaggtc atcattgtgc gcaaccgcta
     9481 ttgaagtacc ttgaagtatc agcgactgct cgcgcgagct tttatttcta caacacaacg
     9541 caagacgccg accgtttagt tgaagcaatc atggcaacaa aggagttttt ccaacatggg
     9601 actatctaaa ttagacaatt tataccgtca agtgatcttg gatcattctt cccatccgca
     9661 tcatcgtgga agcttgactg actcgacaag caagatcgaa ttgaacaacc ccacatgtgg
     9721 agatgtgatc gaattgcaag tcgatgtaaa agacggaatc atcaaaaata tcgcctttga
     9781 cggcagtggc tgctccatca gtacggcaag tgcctcgatg atgacagacg ccgtcatcgg
     9841 aaaatcaata gaagaagcag aacatttaac tgtagacttt ctacagcttg tccaaggaaa
     9901 agaagtcaaa gatcccgacc aattaggcga tgctgctatg cttggtggtg tagctaaatt
     9961 tcccgctcgg atcaaatgtg cgacacttgc ctggaaagca ctaggaaaag caatggaaaa
    10021 cgatggtacc gcagctttcc atcattacca caaggaagag gagtgagaac atgggcgtac
    10081 cagaattaga agaatataaa ttcgggtttc acgatgatgt cgaaccggtt ttcagtacag
    10141 gagaaggctt gacagaagaa gtgatccgtg agatgtcaag aatcaaaggc gaaccagaat
    10201 ggatgcttga attccgtctg aaatctctag aaacatttaa caaaatgcca atgcaaacat
    10261 ggggccccga cctttcagac atcgatttcg atgccatcaa atattaccaa aaaccaagtg
    10321 acaaacccgc tcgtgactgg gaagacgtac cagaaaaaat caaagataca tttgaaaaga
    10381 ttggtatacc agaagctgaa cgagcttatc tagccggtgc ttccgcacaa tatgaatcag
    10441 aagtcgtgta ccataatatg aaagatgaat tcgaaaagtt aggaatcgtc tttactgaca
    10501 ctgactctgc tttgaaagaa taccctgaat tattcaaaga gtacttttca aaacttgtac
    10561 cgccaaccga caacaaatta gcagcgctga attcagccgt atggtcaggt gggaccttta
    10621 tttacgtgcc aaaaggcgtc aaagtcgatg ttccattaca aacgtacttc cggatcaatg
    10681 cggaaaacac ggggcaattc gaacgtacgc tgatcattgt tgacgaagga gcaagcatcc
    10741 actatgtcga aggttgtacg gcacctactt attcaagtaa cagcctgcat gccgccatcg
    10801 tagaaatctt tacaagaaaa gatgcgtatt gccgttacac aacgatccaa aactggtcag
    10861 acaatgtgta caacttagta accaaacgag caaaagcata cgaaggcgca accgttgaat
    10921 ggatcgacgg aaacctaggt gcaaaaacaa cgatgaaata cccaagtgtt tacttagatg
    10981 gaaaaggtgc acgaggcacc atgctctcca tcgcctttgc aggagcaaac caaatccaag
    11041 atacgggtgc aaaaatgatc cacaatgcac caaatacatc tagctctatc gtctctaaat
    11101 caatttcaaa agacggcgga gaagtaaact atcgaggaca agtaaccttt gccaaaaaca
    11161 gtgcgggttc gatctctcat atcgaatgtg acacgatcat catggacgaa tggtcgaaat
    11221 ccgatacgat cccattcaat gagatccaca acagccaagt ctctttagaa cacgaagcca
    11281 aagtctcaaa aatctcagaa gaacaactct actatctgat gagccgaggc ctgaccgaat
    11341 ctgaagcaac tgaaatgatc gtcatgggct ttgttgaacc attcaccaaa gaacttccaa
    11401 tggaatatgc agtcgaactc aatcgattga tcagttatga gatggaaggc agtgttgggt
    11461 aaacctgtta taggagctag aacccttgat atataagggt tctagctttt ttgttttgcc
    11521 tgtatactag aaatgaccag attttgacca gctcttttac tgatgagcca tcatatcagc
    11581 aaagtcttga cctgttttct taggtgcatt atttgtaata tgtgcataga tgtctaatgt
    11641 cactttacta ttttcatgcc ctaatcgttg ttgaactacc ttgacagatt ctcctgcttc
    11701 taataataaa ctagcatgtg tatgtctaaa cccatgaggt gtaatacgag gcaagttata
    11761 ttttttcaaa atatatctta accaatcatt aacagtgttt ggaatgtata gagtgttttt
    11821 aactgttgta aaaacatgtt gctctttaga gctagtattg tatccaagtt ttaaatactc
    11881 ttctttttgc tgtaatttcc agttgttgag gatagaaaga gtctcagtat ctaaagagat
    11941 aactcgtcta gaagctcttg ttttcggaga ttgaattata actttaccaa attcatctac
    12001 agcaagagtt ttattgacat gtagttcttt attaaacgtg tctatgtctt tccactgtaa
    12061 agcgagtact tcactctttc tcatacctgt gtatgccaat aatctgaaga aggcgtattg
    12121 tttcatgtta ccaaaatctt taaaagcatc cagtaaatta ttaagctcct cttttgagta
    12181 aaatttacga tttttctcat actctttttt tcgtggaaga agtgtttttt ccataggatt
    12241 attgtcaata tattgcattg caaccccgta gcgtaaaatt tgtgcagtct gttttctcat
    12301 ataatcgtaa ctttcatatt tactgtgcca ttcatctacc actttttgac aatatgggac
    12361 agagatattc gatagtttaa gcttaccaaa tgctggaagt atttgattat ttgcgtatct
    12421 tactgagatt gcgattgttg aagctttaac actggtacgc tgatgagtta tccattcatt
    12481 atacaggtct tcaaatgtca tatcaggtgc tttcttttta acaccgtata atatttgatg
    12541 tctgtactcg tcataatctt tttgagcttc ttgtttgcta ctaaaacctc ttctcgtagt
    12601 atttattttt tcaccagttc gtggatcggt ggcaatgtgt cctttataca tccacgccac
    12661 ttgaccgttt ttctttttgt actttgatat ttttgccatt aaatcatctc ctcttagttt
    12721 acaaggagta cttaccacta gatggtatca cctcccttca tttttttctt agtgtatttt
    12781 ttcccaattg atgaaatcgt ttgttctagc ttgtttatat tatcagtaat ttctctcaca
    12841 gcttcttcgg gataagacat gtctttcaat tgaataggta gccattcatc taccattctt
    12901 tctagtagtt cttctatact gctagtgagg aaaagaatgt tattgtcaaa ttcttctaca
    12961 atagcattga tgaataattc ttctattttt aagatattga aaagactaat tttttcattt
    13021 ttaactttat tacaagtctt gttagcaata aattcttgca tctctggttc taatttggaa
    13081 tatctatcca gccatgtatc taaattagag tgtactggtg ggaactgttg acgaagatgc
    13141 tggaacaggt ttttatctgg aaatgttata ccatctagta caatgctatc tctataaaga
    13201 ataattccca aaatatattc ttctacattg ccacaaacta actcctctat actcatattt
    13261 cccaaatcag caattcggga taatcgctct ggtattggta aataggttcc cttctcccat
    13321 tcgtagatag tttttttgtc aattaattga ccgttgttaa tcataagacc aaatgctggt
    13381 tgtgttaatt ttttactctt tcttatagct tttatttttg ttccgagtat cttttttcgt
    13441 tctaaataat ttattggtgg acgttttatc atattgacca cacctcctaa aattagtata
    13501 cctcaaaaaa ggggtgttgc aaataaattt tacaaagctg ttgacacccc ttttttatcg
    13561 aggtattatg tgtatgtcga tgaactggta atcatttttt gtttgattaa tcggttaata
    13621 acaaaatact tttattttga gcaaaaaata cgaagataat tcttagaaaa tttgttcaag
    13681 caatcacgaa aggaggggga aatatgtctc tacaaatcat tgatagcatt gtttctatac
    13741 aagttgaaca acaagtgaaa gagataatta cagtgctaaa gcctatgcta gaagacatta
    13801 tttctggagt cactctagag tatatggact atcagcaggc aagtgaatat ctaggtgtat
    13861 cagtaggaac gattagaaaa tatgtttctc aatacgggtt acctgttata aaaattgata
    13921 cggtcattag atttaaaaaa tctgatattg atagtttcat ggaacggtat aaaggttgag
    13981 taaacaaata agactaaaac atatagtaat aaagagtttg tttttattat ctgtatgttt
    14041 tagtctttaa cagaatttta taaggaggaa acacaaatgg aaacatcaat gattaatatt
    14101 ccaatttatg tagatcaaga aaaattatta gagaagccag ttacaaaaaa agaaaaaact
    14161 gatgtaagag ataggtgttt agataactat agagaagtta caagagatga gtttgtagag
    14221 atactaaatt catcacaatc atttattcca agcaagacaa agaataaagg caatgacaag
    14281 gagagttttg tggaaaccag agtaattatt ctggatgtcg ataacactgt taaagatgag
    14341 aaggggaagg tagtagattt gagtaaagat gatagtagat acttaagcgt agaaaaggtt
    14401 ttatctatca atttagttca taattctgcg tttgcgatac aaaaaagtat tcggtactct
    14461 gaaaatctag aaaaatacaa gattgtcttt ctattaaaag aagcaattac tgactatggt
    14521 aaaatggttg ctgtttacga atacttgaaa aagaacatcc ccgggtgtga tgacaatgta
    14581 aatgcttcga atagaatgtt ttttggagga tataagtcag atgcagtgat aataattaat
    14641 gaagacaaca tgttagatat tactgagctt ccgattgatt ttaccatgac atgttctaaa
    14701 aattgtcaaa gtgattcagg gagagagtat gaactagtca atgaaaccga ttttgttaag
    14761 ttgataaaaa atgacgacaa agaagaaatg aaacagtggt ttaaagactg cctgtttgat
    14821 tcctccgaca tgtcttttaa tgagatttat gaaagattgt taaccattga tatgaataaa
    14881 ttacttaagt caagtaaaaa cttaagatgt ctgtttcaca acgatcataa tccctctgct
    14941 agtattttta ccagtaaaac tgggcattcc attttttatt gtcattccag taattgtgat
    15001 atcagtgaaa attttattgg agttgtgatg ctaattttaa agaaagacag tcgggtagaa
    15061 acatttaatt ggctattaga gaacctagac ttgtatccta cacattttcg aaaattgaaa
    15121 gaggaatcaa aagtactttt tgacactctt gaatcaatga aagacaaact tttcaggaca
    15181 ataaggaatt atctagagga aatgcgacgt gtctatgatg tcttgctttc tgatgttaat
    15241 ttttttgatg atagtaagga aagtgtgact tgtattttgt cgggggaaca attagctaaa
    15301 aaaatgagtt cttactatgg taaaacttat gacatagata aatgtaataa gttactatca
    15361 cttatgactt ttataggatt gataaaaaaa ttagatgatg aagatgttcc tttgaagatg
    15421 ctcgaatacc taaataggtt aaaggaagag aaagcgttag gaaactttaa tcataagaga
    15481 agcaatgtct atcgattaga cgtttttaat gcaacttctg tgattcaaaa attgaatgaa
    15541 gatatccttc cacttctgga aaaaattcat tttacatatc aatatttttc ttatgaatgg
    15601 gttaagatat gttttaacga aaaagaggct aaaagagtat ttcctcaaaa ttcccgatca
    15661 gaactcagca aggaaaaaca attgatttta gatgaagcac atgaagtttt acagggctta
    15721 tcgtggcaag ggacatatct aatgacagag aaagaactta ttaacactgt ttgtgaacgc
    15781 ctacgtaatg ttggggataa cagcgtgaaa aatgctttaa taaaaaacag aggctacttt
    15841 gttaaacaag gattcactct tgaacgagct tctaaggata ttaagaaact atacaatgtg
    15901 aaagcaagta gtagtttttt aatatattta gcgaaagggg cagggataga tgaaacaata
    15961 aaagaattga tattatcagg agctgggata gttcctctgt atcaatttac aagtgtaaca
    16021 agagatgatg gacggagagt aaggaagaga aaagacccaa ttgtattaaa ctagacaggt
    16081 tatccatagc tcttaatctt atttcatctc aatacaccca ccgttaaagt tcggtgggtt
    16141 atttattttt caatctttca atagctgatt gcgttcattt tcttgccttt ttaatagctt
    16201 catatagtca attgttcgaa ctgcatcttc agtgctaaga gaacgataga gagccaaaaa
    16261 ctcttcctca taatctacga caacattgtc tagaaaatgg cttacagggg ttcctagggc
    16321 tctagaaaga gctgttaagc tatcgaggtt aggctctgtt acagaatttt cccatcttgc
    16381 tactgcctgt tgtgtagaga agattttttc agctaattct ttttgtgtcc acccgagagc
    16441 ttttcgcttt tctctaatga cctttgcata ttgtttctgt ttcattaata cacccccatc
    16501 tcagtatagt acaacaagtt gttgtatttg taatggtatt tgaagttttt tatattttat
    16561 gagttgaaat acatcatatt gttgtatata ataaaacagg aggggagaat catgaaaaaa
    16621 gtaagtaatc tcagccgata cagaaaagaa aaaggacttt ctcaaacgga gttagcaaaa
    16681 aaaatgaatg ttacacagca atgtgtaagt tcgtggcaaa cagggagaac gattcctaaa
    16741 ccctatcaaa tgaagatgct ttctgagata ttaagtgtac caatgaatga gctgttttct
    16801 gatgtattta ataaagtaaa cagttaacta ataagcaatt gaatgatttt aggaggagaa
    16861 gacatgtatt gtagaaaatg tggcaaagaa ataacagatg aaagtaagta ttgccagttt
    16921 tgtggagcac tacaagaaac agatgagaca accggaacag acacagtaaa gaaaagtact
    16981 gagcagacaa ttaatgaaat gaaggaacaa atcgtacatg gacaagagac agggaagctg
    17041 tatttgatta ttggttggat ttctatggta gtatcgttag catttattcc tgttgtattt
    17101 ggtgctgttg ctgttattat gggctactta tatcgagaaa aagatgagaa aatgggtacg
    17161 attttgatga tagcaggggt agctggagca atctttggtg tacttttagg gatgtcaatg
    17221 tactgataag agggataaga gcaaattatt ttggagatag gagataaaaa gttgaataaa
    17281 gtaaatatga acaagaagtt tgtcattata ggtattgttg tcttggctat tataggtatt
    17341 attttttcaa aaatgacagg tacatctgga ttagagaagt catcaaaacc attggtaaca
    17401 cagattttac aagaacagta caacttaaat cgatcatgtg atgatgtgac gattactcat
    17461 gaaaatggag ataacacata ccgggctaaa gctatattag acaatggaag tgctatcaac
    17521 ataaatattg agtactatcc aaagaaagac cgtatatatg tggagatacc atatgctgag
    17581 gtgttaatgt taaattgatt taaatgtatt atgcataccg tccaaacagg aactcccaaa
    17641 ggtgtaaatt cactagggag tttttatagt ttcctataaa ttaaatacta atacaggtga
    17701 caaaaaaata ttacaatgat tgtcgggaat ttttatgtta ttcactgtta taacaatata
    17761 aaagaaatga aaaagaaaaa attattatgg atatgtttgg tattttt
//
LOCUS       DACDKM010000050        17344 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20834_2016
            SAMN12640370-rid9891663.denovo.050, whole genome shotgun sequence.
ACCESSION   DACDKM010000050 DACDKM010000000
VERSION     DACDKM010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640370
            Sequence Read Archive: SRR10040891
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17344)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17344)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742835.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742835.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:04:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,012
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 29 of 75
            Pseudo Genes (incomplete)         :: 33 of 75
            Pseudo Genes (internal stop)      :: 19 of 75
            Pseudo Genes (multiple problems)  :: 5 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17344
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640370-rid9891663.denovo.050"
                     /isolate="E20834_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            1..368
                     /locus_tag="IV334_001577"
                     /pseudo
     CDS             1..368
                     /locus_tag="IV334_001577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329268.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
     gene            548..1489
                     /locus_tag="IV334_001578"
     CDS             548..1489
                     /locus_tag="IV334_001578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-related sulfurtransferase"
                     /protein_id="HAP8943650.1"
                     /translation="MDYQVLLYYNYTKIEEPEEFAKNHLAFCKSLDLKGRILVAEEGI
                     NGTVSGTVENTTAYMKAMKADPRFADTMFKIDPSEGHAFKKMFVRYRPELVSLKLEED
                     IDPNQVTGAYLSPQEFKEAILDEQTVVIDARNDYEYDLGHFRGAVRPDIRSFRELPDW
                     IREHKEEFMEKRVVTYCTGGIRCEKFSGWLVREGFKDVGQLHGGIATYGKDPEVQGEL
                     WDGKMYVFDERIAVDINQVDKQIVGRDWFDGTPCERYINCSNPECNRQILASIENEEK
                     HLGACSIECAKHPRNRYVQDHGISPEEAELRFTALIK"
     gene            1558..2289
                     /locus_tag="IV334_001579"
     CDS             1558..2289
                     /locus_tag="IV334_001579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine protein phosphatase"
                     /protein_id="HAP8943651.1"
                     /translation="MKSLIYAIGDVHGKHELFEKLIHYYDPKIHQLVLLGDLNDRGPR
                     TKECFLLGKQLVEETGAIYLRGNHEEYFLQFLQSPEDWYAPYMRNGGKETFESLIHPG
                     ASEEYSPTEISMMIQSRYSELIKFLVQRPLYYEWENYLFVHAGVDLTKKDWRKTSLRD
                     FIWIREPFHEGKNNTGKTIVFGHTITPMLHGDMQTTDLWIQDHKIGMDGGAVFGGSLH
                     GVVFDKEGILQDIEYQNNEGPWHGI"
     gene            2433..4613
                     /locus_tag="IV334_001580"
     CDS             2433..4613
                     /locus_tag="IV334_001580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding transcriptional accessory protein"
                     /protein_id="HAP8943652.1"
                     /translation="MTENLNQTIIQLVQKELSDYRPKQLTTVLNLLNEGNTVPFIARY
                     RKEMTGSLDEVQIREIEERYAYLENLEKRKTEVIRLIDEQGKLTPELEAEITQAVKMQ
                     QVEDLYRPYKQKRRTKATIAKEKGLEPLAKWLMQLTDGEVQSEAEKYIDKEKEVSSAE
                     EALHGAHEIIAEQVSDNAKFRTWIRSYTYNKGMYVSNVKDEQADEKGVYEMYYDFAEP
                     VHKMVSHRILATNRGEKEDILKVFFQVDEAAILAYLDRQIVKNPASPSSSFVREAYQD
                     SYKRFIQPAIERELRNELTEKADEQAIAIFGENLRNLLLQPPLKGKVVLGFDPAYRTG
                     CKLAVVDATGKVLAIEVIYPHKPAAQAKREAAGPAFIQLINKYQVDMVAIGNGTASRE
                     SELFVAEQLKSADHKTYYAIVNEAGASVYSASEIARKEFPHLQVEERSAVSIARRLQD
                     PLAELVKIDPKAVGVGQYQHDVSQKRLAEQLDFVVETAVNQVGVDVNTASPQLLQHIS
                     GLNKTTAQNIVIYREENGEFTARTQLKKVPRLGPKAYEQAIGFLRVPGGKNILDNTGI
                     HPESYSIAKEILTSVQLSEKELGTEEAVEKLTRLSVEKLAESLSVGEETLADILAGLT
                     QPGRDMRDEMPAPLLRTDVLSMEDLKPGMELTGTVRNVIDFGAFVDIGVKQDGLVHIS
                     KLSKKFVKHPTDVVSVGDIVTVWIEQVDTKKGRISLTMLSPYEE"
     gene            4633..5115
                     /locus_tag="IV334_001581"
     CDS             4633..5115
                     /locus_tag="IV334_001581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family protein"
                     /protein_id="HAP8943653.1"
                     /translation="MTQEELQQLVEEISLKSFQLPFKHQAMFNPRLRTTGGRYHLNDH
                     HLDFNAKLFEESDEETIAGIIKHELCHYHLHLAGRGYQHKDMDFKYLLKKTGGLRYAP
                     ALTKKQIKMEYYRCQDCSATICRKRRIDVRKYRCGRCHGEIKWIETKREDFNEAQKIR
                     "
     gene            5131..5892
                     /locus_tag="IV334_001582"
     CDS             5131..5892
                     /locus_tag="IV334_001582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HAP8943654.1"
                     /translation="MEKSEQRNLKDWNQLLNFLAAPEEGNFEELTAPTLILAGNCLPI
                     LADKAAQMYLNGQVDQLFLVGGVGHATRILYENFEKQGFHFEDGMSESEICRQYLKEV
                     YDLPDKAFLIESKSTNSGENATFSLEILHSLGAVPEKILLMNDPTLQRRTRATFEKVW
                     QNEQTIFANAVPFVPEILHFSEEIIFTAKELNHQWPKEYFHALVLGEMERLHDDENGY
                     GPKGKDFIPHIDISEEVWSSYTRIKESIKTDFSRT"
     gene            6023..7357
                     /locus_tag="IV334_001583"
     CDS             6023..7357
                     /locus_tag="IV334_001583"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HAP8943655.1"
                     /translation="MENQNKLYHFVGIKGSGMSSLALVLHEQGLNVQGSDIEKYFFTQ
                     RDLEKANITILPFNADNVKPGMTIIAGNAFPDSHEEIQRAKELGLEVIRYHDFIGHFI
                     QNYTSIAVTGSHGKTSTTGLLSHVLSGVRPTSYLIGDGTGHGDPQAEFFSFEACEYRR
                     HFLAYSPDYAIMTNIDFDHPDYYTSIDDVFTAFQTMAGQVKKAIFAYGDDAYLRKLKA
                     NVPIYYYGVTENDDIQARNIERTTSGSAFDVYHGDEFVGHFTVPAFGKHNILNALGVI
                     AVAYFEKLDLKEVAEEMLTFPGVKRRFSEKIVADMTVVDDYAHHPAEIKATIDGARQK
                     YPDKEIIAVFQPHTFTRTIALMDEFAEALDLADKVYLCDIFGSAREEQGNVKIEDLGA
                     KIKKGGEVIKENNVSPLLDYHDAVVIFMGAGDVQKFEQAYEKLLSSTTKNVL"
     gene            7785..8270
                     /locus_tag="IV334_001584"
     CDS             7785..8270
                     /locus_tag="IV334_001584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dehydratase"
                     /protein_id="HAP8943656.1"
                     /translation="MNIGKPRKLGKTINEIEEGDSLSLTESIEDNQLLLYLGLTNDAN
                     PLYIQHDFAQKTEYQQPIVPSVMLMGIITSAISKHLPGPGSHVVNFSVNFIEPVFHYE
                     TLTFQFEVIKVDKMKDVVTISVEAENTNEDRVLDAVVMVQPPQVPELEIAIESEGELN
                     E"
     gene            8263..8946
                     /locus_tag="IV334_001585"
     CDS             8263..8946
                     /locus_tag="IV334_001585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HAP8943657.1"
                     /translation="MNNPSTSMSGINRFYAKVYGFLGMGLALSAVTSYLALWVFPLQL
                     ATFINNFPLGFVGLWIVELILVMVLGVKAQKNPSLAIGGFIAYSLLNGITLAVTLAYY
                     DIGTVTQAFLTATLMFMGMSVVGVTTKRDLSGVGRAGQAALIGVIIALVLNIFILRSS
                     VVELFISLLLVVIFAGITAYDNQSIRNIYVQTNGTAHSGIAVFMALKLYLDFINLFLA
                     FLRIFGRNN"
     gene            9072..11717
                     /gene="polA"
                     /locus_tag="IV334_001586"
     CDS             9072..11717
                     /gene="polA"
                     /locus_tag="IV334_001586"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HAP8943658.1"
                     /translation="MTKNKLLLVDGNSVAFRAFFALHNSLERFKNRNGLHTNAIYAFN
                     TMFENVMQKEQPTHVLVAFDAGKTTFRTEMYAEYKGGRSKTPGEFKEQMPYIRDLLTG
                     LGVQYYELPNYEADDIIGTLAEKVDKDQFDVVVLSGDRDLTQLASKEVKVDITVKGVS
                     DIESYTPEHVAEKYDGLTPKQIIDMKGLAGDASDNIPGVTKIGEKTAIKLLKQYGSVE
                     GIYEHIDEMKQSKMKENLINDKEQAFLSKKLATINTSAPVDVSIDSLKYEGKNLDKLV
                     PFYKEMDFNQFLSKLNIVEEEVKMDDILFEVVHEFKEEMFTTDMALYVEMMGDNYHTE
                     EIVGVAWGTEKKIYVTNDLSVFENRAFHSWITDPTRLKKVYDAKRTYVALNRYTGKTK
                     GIDFDVLLGAYLLDTNEKSTDIEGIAAHYGYNDIQSDEAVYGKGAKKGLPEEEELFFA
                     HLARKVAAINALTPKLSQELADKNQEDLFRKMELPLSIILAEMEISGIKVDATRLQEM
                     KGEFSARLREIEQKIYEEAGEEFNLNSPKQLGVILFEKMGLPVIKKTKTGYSTAVDVL
                     EQLREQAPIVEDILTYRQIAKIQSTYVEGLLKVIQSDGKVHTRYVQTLTQTGRLSSVD
                     PNLQNIPIRLDEGRKIRQAFVPREKDWLIYSSDYSQIELRVLAHISNDEHLKAAFLEG
                     QDIHASTAMRVFGIEKAEDVTPNMRRQAKAVNFGIVYGISDYGLSQNLGISRKAAQQY
                     IDTYFEKYPGVKEYMERIVREAKDQGYVETLYHRRRYLPDINSRNYNIRSFAERTAIN
                     TPIQGSAADILKIAMIELDKRLKETGLQATMLLQVHDELVFEVPEKELESLDKLVKEV
                     MEQAVSLHVPLITDSSWGKTWYEAK"
     gene            11762..12598
                     /gene="mutM"
                     /locus_tag="IV334_001587"
     CDS             11762..12598
                     /gene="mutM"
                     /locus_tag="IV334_001587"
                     /EC_number="3.2.2.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase"
                     /protein_id="HAP8943659.1"
                     /translation="MPELPEVETVRKGLERLVVGKTIQKVQVLWPKIIEQPETPIFEA
                     SLVGETIQSIGRRGKFLIFHLDHCELISHLRMEGKYQFTKENTPIDKHTHVLFFFEDG
                     SQLRYNDVRKFGRMTIVEKGASATYRGIMKLGPEPLPDSFLLADFANGLKKSHKAIKP
                     LLLDQRLVTGLGNIYVDEALWEAKIHPEQPANTLRPKEVEQLRLSIIDVLDRAIEAGG
                     TTIRSYLNALGESGGFQVALHVYQQTGKPCIRCGTPIVKTKVAQRGTHYCPNCQRLKI
                     AK"
     gene            12562..13191
                     /locus_tag="IV334_001588"
     CDS             12562..13191
                     /locus_tag="IV334_001588"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HAP8943660.1"
                     /translation="MSELSKAKNRKVGFVLGLTGGIATGKSTAAKVFQSHGFPLIDGD
                     VIARETVEPGTLALRKIVSVFGQEVLQPDGQLDRGKLGMIVFPSKEKRQKLDQLLDPF
                     IRKAIKDQIASLSSNHPLVIVDIPLLYEGHYDHYMDAVAVVYTTPETQLQRLMKRNQL
                     TLEQAQQRISSQLPIEEKKQRADILFDNNGTKEKLVGQIENWLFANRFL"
     gene            13243..13575
                     /locus_tag="IV334_001589"
     CDS             13243..13575
                     /locus_tag="IV334_001589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HAP8943661.1"
                     /translation="MGSLKNQKDKVVGNAKEKIGEMLNKENLIDEGRTQSETAEEKEK
                     QYQARIDEIEDQQAEEEAKRQDLTQERKNPTPDRSKTPLPESDHISDEQKIEEQRMKH
                     YTGIEEKL"
     gene            13887..14378
                     /gene="nrdR"
                     /locus_tag="IV334_001590"
     CDS             13887..14378
                     /gene="nrdR"
                     /locus_tag="IV334_001590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor NrdR"
                     /protein_id="HAP8943662.1"
                     /translation="MRCPRCQHNNSRVIDSRQADDGRAIRRRRECENCSFRFTTFERI
                     EAAPLLVIKKNGEREEFNRDKILRGLIRSAEKRPVAMEQMEQIVNHVENRVRSLGENE
                     VSTNLIGEYVMEDLVDLDEIAYIRFASVYRQFKDMSVFLKELQDIVDKAKENNKENNK
                     ENN"
     gene            14401..15774
                     /locus_tag="IV334_001591"
     CDS             14401..15774
                     /locus_tag="IV334_001591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Replication initiation and membrane attachment"
                     /protein_id="HAP8943663.1"
                     /translation="MENAWKELQPKNIYQGRKAAPLREDSKNALLYLYQPLIGGEALS
                     IYFTLLSEISQESGKGPEGMHADLLSSLGCGIPQFYDARKKLEGIGLLDVYFKEDSDL
                     GPRFIYELLEPLIPKAFFKDSVLAFLLLEKVGERRFQKLTQFFEPKRHTAEGYQKITK
                     KFLDVYRFNEAVYAANQENIESLNKSFEESESVALLEADSTFDWQFLNDWLAKKHIAS
                     LDDPIIKQVDMYHQLYGFDELTLGELISQSYDFSEQKVSEKELQKAILAMQRRPVKQT
                     KETVAVSENSNEPSSNEHLTNAALQLIKEAQSIPPMKYLEAIKSEKGGYVSKQENWLL
                     QDLVSQSGLSSSVINVLLNYVLVIKNNASLNASYVNTVANEWAQKKIATAEEAIQHIR
                     QISNQAKQSKQKKQTNYQTGKRNVRREKLPDWVNQPKDEKQISSEKKAEIDRRFKEYL
                     AKKEGDI"
     gene            15774..16700
                     /gene="dnaI"
                     /locus_tag="IV334_001592"
     CDS             15774..16700
                     /gene="dnaI"
                     /locus_tag="IV334_001592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein DnaI"
                     /protein_id="HAP8943664.1"
                     /translation="MEDVGKELKRIISKRNYQEKYAEMIAEVLNDADVQAFLSAHQEE
                     LTQADIEKSYAKLYEFVQEKRKYQKNDPTMIAPGYEPKLTLNFHYVDVTYVPTEALLA
                     RQRQEEIRSRVKAMDMPKDIQSASFENFERTDGRGYASLEALDFVEKYTADPKGYHKG
                     LYLVGSFGIGKTYLLGATARELAIAGFTTTLVHFPTFAVEMKQAIGKDQVAEKLDAVK
                     RSPILMIDDIGADAMSSWIRDEVFGVILQYRMQEQLPTFFSSNFTMDELEQHLSVTQR
                     GDEEPLKAKRIMERIRYLTKEIEMTGRNRRNG"
     gene            16829..17269
                     /locus_tag="IV334_001593"
     CDS             16829..17269
                     /locus_tag="IV334_001593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HAP8943665.1"
                     /translation="MRAIFSSISVVGLFFLLAGCGSADSNTGSVDSIDAGQTVKTSST
                     KTERQSIFVGTIESITQVEAEEETLQLKLVDVMAKEDPANMGTSFSNDGVILNATAEK
                     ISVDINELKIGDKVQFTLVEMPIMTMSIPPQVPGDSIIDVSLNK"
BASE COUNT         5908 a         3032 c         3820 g         4584 t
ORIGIN      
        1 atgtttacga atgaaataaa aatcatgctt tatgtggacg atgtagaaaa aaatgcaacg
       61 ttttggagag cagttggctt tcggaaaaag atcgtcaaga aatggatggg acgcttataa
      121 ttgaagtagg agttgaggac agtcaaacct catttctgtt atatgatcga gcatttgttg
      181 agtcccattc acctggcaca gctactagtg ccccatcttt gatgttttac agtaaagata
      241 tctttggact atataaaaaa atgcagacgc aatcagtcac attaggtgat atggtacagc
      301 ttggagaaga atatgtcttt aactttgctg atcccgatgg caactacttt gcggttacag
      361 gtaaataata gaaataaaga ctttgttttt ctgtgcgaaa caggaaagcg aagtcttttt
      421 aattcaaata ataatattat gtaaacaaaa aactttccat gcgtaattat acacaagcaa
      481 ataaatcgtc aaatgcgaac gccttcttta tactgtttag gaacgaaaag aaggaaggaa
      541 gaaatacatg gattatcaag tactgcttta ttacaattat acaaaaatcg aagaaccaga
      601 ggaatttgcc aaaaatcatt tagccttttg caaatctttg gatcttaaag gtcgtattct
      661 agtagctgaa gaaggaataa atggtactgt gtctggaacc gttgaaaaca cgactgccta
      721 tatgaaagca atgaaagctg atcctcgatt tgctgatacg atgttcaaga tcgatccaag
      781 cgaaggacat gcctttaaga agatgtttgt aagataccgt ccagagcttg taagtttaaa
      841 gctagaagag gacattgatc caaaccaagt gacaggtgcc tatctttcac cgcaagaatt
      901 taaagaagct attttagatg aacagacagt ggtgattgat gcacggaatg attatgaata
      961 tgacttggga cattttcgag gggctgtacg cccagatata cgcagttttc gagagcttcc
     1021 agactggatc cgcgagcaca aagaagaatt tatggaaaaa cgggtagtga cctactgtac
     1081 tggtggcatt cgttgcgaga agttttctgg ttggcttgtc cgtgaaggat tcaaagatgt
     1141 cggacagctg catggcggta ttgctactta tggtaaggat cctgaagtcc aaggagaatt
     1201 atgggacggc aagatgtatg tgtttgatga acggatcgct gtcgatatca accaagtaga
     1261 taaacaaatc gtcggtcgcg actggtttga cggaacacca tgtgaacgat atatcaattg
     1321 tagcaatcct gagtgcaatc gtcagattct tgcttccatt gaaaatgaag aaaaacattt
     1381 aggagcatgt agcatagagt gtgcgaaaca tccgcgcaat cgctatgttc aagaccatgg
     1441 aatctctcca gaagaagcag aactgcggtt tacagcttta attaaataga aaacaatagt
     1501 tttgattgtc gaccatttcc aatatgcgct atactagcgt tgaggatggt gatccacatg
     1561 aaatcattga tttatgctat tggggatgtc catggcaaac atgagctttt tgaaaaactt
     1621 attcactatt atgatccaaa gatccatcag cttgttttat taggggatct caatgatcga
     1681 ggtcctcgta caaaagaatg ttttttatta ggaaagcaat tagtcgaaga aacaggagcc
     1741 atttacctcc ggggaaacca tgaagaatat tttttgcaat ttctgcaatc accggaggac
     1801 tggtatgcgc cttatatgag aaatggtgga aaagaaacgt ttgaaagcct gattcaccca
     1861 ggggcatcgg aggaatactc cccaactgaa atctcgatga tgatccaatc gagatattca
     1921 gaactgatta aatttttagt ccagcgcccc ttgtattatg aatgggaaaa ttatcttttt
     1981 gtccatgccg gtgtggattt gacaaagaaa gactggcgta agacaagtct aagagacttt
     2041 atttggataa gggaaccatt tcatgaaggg aaaaacaata caggtaagac aattgtattt
     2101 ggacatacga ttacaccaat gctccatgga gatatgcaga caacagacct ctggattcag
     2161 gatcataaaa tcggaatgga cggtggggct gtattcggag ggtcattgca tggggttgtt
     2221 tttgacaagg aaggtattct tcaagatatt gaatatcaga acaacgaagg accttggcat
     2281 ggaatctgac taatggaaag aatgaaggag ctgtgacttt gttgcggctc tttttatttt
     2341 tatagagaat attgtttctt tcagtagttt aacccaggat aaaaagtatg atagaataaa
     2401 gaagcaattt ttagcagaaa agaggcattt taatgacgga aaatttgaac cagacgatca
     2461 ttcagcttgt acaaaaagaa ctttcagact atcgtcctaa gcagttgact actgtactta
     2521 atttgttaaa tgaaggaaac actgttcctt ttatcgcgcg ttaccgaaaa gaaatgactg
     2581 gaagtcttga tgaagtccag attcgtgaaa tcgaagagcg gtatgcctat cttgaaaact
     2641 tagaaaaacg taaaactgaa gtgattcggc taatcgatga acaagggaaa ctgactcccg
     2701 agttagaagc agaaatcacc caagccgtga aaatgcaaca agtggaagat ttgtatcgtc
     2761 catacaaaca aaaacgacgg acaaaagcaa cgattgctaa agaaaaagga cttgaaccac
     2821 tcgcgaagtg gctgatgcag cttacagatg gagaggtgca atctgaagca gaaaaatata
     2881 ttgacaaaga aaaagaagtc tcttctgccg aagaagcgtt gcacggtgct catgagatta
     2941 ttgctgaaca agtcagcgat aatgcaaaat ttcggacttg gattcggtct tatacgtata
     3001 acaaaggtat gtatgtcagc aatgtcaagg atgagcaagc agatgaaaaa ggcgtatatg
     3061 aaatgtatta tgactttgca gaacccgtcc ataaaatggt ttctcatcga atattagcta
     3121 ctaatcgtgg agaaaaagaa gatattctga aagtattctt tcaagtagat gaagcagcaa
     3181 ttttagctta cttggatcgc caaatagtca aaaatcctgc ctctccttct tcttcattcg
     3241 tcagagaagc ctatcaggat agttataaac gctttattca gccagcaatc gagcgagaac
     3301 tgcgcaatga attgactgaa aaggcagatg agcaagcaat cgctattttc ggcgaaaacc
     3361 tccgtaatct tttgttgcaa ccgccactaa aaggtaaagt tgtcctggga tttgatccgg
     3421 cttatcgtac tggatgtaaa ctagcagtcg tagatgcgac agggaaagtt ttagcaatcg
     3481 aagttattta tcctcataaa cctgcagcgc aagcaaaaag agaagctgct ggaccagctt
     3541 ttatccaatt aattaataaa tatcaagtag atatggtagc tatcggaaat ggtacagcca
     3601 gtcgggaatc tgaacttttt gtggctgagc agttgaaatc cgccgaccat aaaacctatt
     3661 acgctatcgt caacgaagca ggcgcttctg tttactccgc tagcgaaatt gcacgaaaag
     3721 aatttccgca tctacaagtt gaagaaagaa gtgctgtttc tattgcccgt cgcctgcaag
     3781 atcctttagc tgaattagtc aaaatcgatc ctaaagctgt cggagtaggc cagtaccaac
     3841 atgacgtttc tcaaaaacgt ttggctgaac aattggattt tgttgtggaa acagcggtta
     3901 accaagtagg tgtggatgtg aatacagcaa gcccgcagct cttgcagcat atatcaggat
     3961 tgaataaaac aactgcccaa aacattgtca tttatcgtga agaaaacgga gaattcaccg
     4021 cacgaactca attgaaaaaa gtgccacgct taggaccaaa agcatatgaa caagcaatcg
     4081 gattcttgcg agtccctggt gggaaaaaca ttttggacaa cacaggtatc catcccgaaa
     4141 gttattctat tgcaaaagaa atattaactt cagtccaact ttcagaaaaa gagcttggta
     4201 cagaagaagc agtagaaaaa ttgacacgat tatccgtaga gaaactggct gaaagcctat
     4261 cagtcggaga agaaacgtta gccgatattt tagctgggct gacacaacct ggacgagata
     4321 tgcgtgatga gatgccagcg cccttattac gtacagatgt attgagtatg gaagacttga
     4381 aaccagggat ggaattgact ggaacggtcc gtaatgtcat tgacttcggc gcatttgttg
     4441 atatcggtgt gaagcaagac ggacttgtcc atatatcgaa actcagcaaa aaattcgtca
     4501 agcatcctac agatgtcgtg tcagtaggag atatcgtaac agtttggata gaacaagtcg
     4561 atacgaaaaa aggcagaatc agtttgacga tgctttcccc ttatgaagag taacaatgag
     4621 gagtaacaaa aattgacaca agaagaactg cagcagttag tagaagagat atcattaaaa
     4681 agcttccagc ttcctttcaa gcaccaagca atgttcaatc cacgcttacg tacaactggc
     4741 gggcgctatc atttaaacga tcatcattta gattttaatg cgaagctatt tgaagagtca
     4801 gacgaagaga cgattgcagg aatcatcaaa catgaacttt gccattatca tcttcatcta
     4861 gctggaagag ggtatcagca taaagatatg gatttcaagt atcttttgaa aaaaacagga
     4921 gggttgcgct atgcaccagc cttgacgaag aaacagataa agatggagta ttatcgatgc
     4981 caagattgtt ctgccacgat ctgccgcaag agaagaatcg atgtacgcaa atatcgttgt
     5041 ggaagatgtc atggagaaat caaatggatt gagacaaaaa gagaagactt taatgaagca
     5101 caaaaaatca gatgaaagga tagaaggcct ttggaaaaaa gtgagcagag gaatctaaaa
     5161 gactggaatc agctattaaa ttttttagcc gcacctgaag agggaaattt tgaggaactg
     5221 acggcaccta cacttatttt agctggaaac tgtcttccta tcttagctga taaagcagca
     5281 caaatgtatt taaatggcca agtagatcaa ttgtttttgg tcggaggggt ggggcatgcg
     5341 actagaatcc tttatgagaa ttttgaaaag caaggatttc attttgaaga tggcatgagt
     5401 gaaagtgaaa tctgtcgtca gtatctaaaa gaagtctatg atttgcccga taaggctttt
     5461 ctgatagaat cgaagtctac aaattctgga gagaacgcca ctttttcact ggagattctt
     5521 cattccttag gagctgtacc agaaaaaatc ttattgatga atgatccgac actgcaacgc
     5581 aggactcgag caacttttga gaaagtatgg caaaatgaac aaactatttt cgcgaatgct
     5641 gtgccatttg tgccggaaat ccttcacttt tctgaagaga tcatctttac agcaaaagaa
     5701 ctgaatcatc aatggcctaa ggaatatttc catgcgctag tattaggaga aatggaacgt
     5761 ctccatgacg atgagaacgg ctatggtccg aaaggaaaag attttattcc tcacattgat
     5821 atatcagagg aagtttggtc ttcttacacc agaattaaag agtcaataaa aacggatttt
     5881 tcccgtactt gaccatttta gcaagcgaat cctcctttcc aaataaattt ggttgaaatt
     5941 ttcaatcgtt tataagataa tgaggagcgt gttgaatgta atagctaaaa gaatggacgc
     6001 tagataagag aagaggattc aaatggaaaa tcaaaataaa ttgtatcatt ttgttggtat
     6061 caaaggttca ggaatgagct ccttggctct tgttttacat gaacaaggtc taaatgtcca
     6121 aggatcagac attgaaaaat atttctttac acaaagagat ttagaaaaag caaatatcac
     6181 gattctgcca tttaacgcag ataatgtaaa accagggatg acgatcattg caggaaatgc
     6241 atttcctgat tcacatgaag aaatccagcg agcaaaagaa ttaggtttgg aagtgattcg
     6301 ctatcacgac tttattggtc attttatcca aaattatacg agtatcgctg taacaggctc
     6361 tcacggaaaa acaagtacga ctggactgct ctctcatgta ttgagtggtg tccgtccaac
     6421 aagttatcta attggagatg gaacaggaca tggtgatccg caagcggaat tcttttcatt
     6481 tgaggcctgt gaatatcgcc gtcatttcct tgcctattca ccagactatg caatcatgac
     6541 caatatcgat tttgatcatc cggattatta tacaagtatc gacgatgtat ttacagcttt
     6601 ccaaacaatg gctggacaag tgaaaaaagc tatttttgcg tatggtgacg atgcttattt
     6661 acgcaaatta aaagcaaatg tgccgattta ttattatggt gtcacagaaa acgatgatat
     6721 ccaagcacga aacatcgaac gcacaacttc tggttctgct tttgatgtct atcatgggga
     6781 tgaatttgtt gggcacttca ctgtaccagc attcggtaaa cacaatatct taaatgcttt
     6841 aggtgttatt gcagtagctt atttcgaaaa actggatctg aaagaagttg cagaagaaat
     6901 gctgactttc ccaggagtaa aacgtcgatt cagtgaaaag atcgtagcag acatgaccgt
     6961 agtcgatgat tatgcgcatc accctgcaga gatcaaagca acgattgatg gcgcacgcca
     7021 aaaatatcca gataaagaaa taattgcagt tttccaaccc catacgttta cgcgtacgat
     7081 tgcattgatg gatgaatttg cagaagcact tgatttagct gataaagttt acttatgcga
     7141 tatttttggt tcggctcggg aagaacaagg aaacgttaag attgaagacc ttggagcgaa
     7201 aatcaaaaaa ggtggagaag tcatcaaaga aaataatgta tctccattgt tagattatca
     7261 tgatgcagta gtaatcttca tgggtgctgg agatgttcaa aaatttgaac aagcgtatga
     7321 aaaactttta agcagcacga cgaaaaatgt actgtaatac aattactgca gtgtccttat
     7381 aaaaatagaa agagggggag tggcaaaaat cttgccaagc cccctttttt gcaacttatg
     7441 ataaacggtg ttcaaaagct tgcatctgcg gtaataagcc caatcaaatc aaaaacatga
     7501 gaagctgttt ttcttggctg ttcttattac ttgatgcagg gcagcttttt cacgacctca
     7561 gataaacggt gttcaaaagc ttgcatctgc ggtaataagt ccagccaaat gaaaaatatg
     7621 agaagctgtt ttttcttggc tgttcttatt atttgatgca ggacagcttt ttcacgacct
     7681 ctgtaaacta taggaatctg ttaaatcaag ggagcagaac ttgcctttta cgctactttt
     7741 ggtacaataa tgtatgatta aaaataaact ggagggtttc tatcttgaac ataggtaagc
     7801 cgagaaaact aggaaaaaca ataaatgaaa tcgaggaagg cgactcgtta tccttaacag
     7861 aatctattga agataaccag cttttgctgt atttgggatt gacgaatgat gcaaatcctt
     7921 tgtatatcca acatgacttt gcacaaaaaa cagaatatca acagccaatc gttccatcag
     7981 tgatgctgat ggggatcatc acaagtgcaa tttcaaagca tcttcctgga ccaggatcac
     8041 atgttgtcaa tttttctgtc aattttattg aaccggtttt ccattatgaa acactgactt
     8101 ttcagtttga ggtcataaaa gtagataaga tgaaggatgt ggtgacgatt tctgtagaag
     8161 cggagaatac gaacgaagat cgtgtgttgg atgctgtcgt tatggtccaa ccgccacaag
     8221 ttcctgaatt ggaaatagca atcgaaagtg aaggtgaatt gaatgaataa tccaagtaca
     8281 tcaatgtcag gtatcaatcg tttttatgca aaagtttatg gatttttagg tatgggactt
     8341 gcccttagtg cagtcacttc ttacttggct ctttgggtat ttcctttgca attagcaact
     8401 tttatcaata attttccact aggctttgtg ggtctgtgga tcgttgaact gattttagta
     8461 atggttctag gcgtaaaagc acaaaagaat ccgagcttag caatcggtgg atttattgcc
     8521 tactctcttt tgaatggcat aacattagcg gttactcttg catactacga tattggaaca
     8581 gtaacgcaag ctttcttgac agctactttg atgttcatgg ggatgtctgt tgtcggagta
     8641 acaacaaaaa gagatcttag cggggttgga agagcaggac aggcagcttt aatcggagtg
     8701 atcattgcac tagtcttgaa catcttcatt ttgagaagta gcgtagttga gctgttcatc
     8761 tccctactgt tagtcgtcat ctttgcaggg attacagctt atgataacca aagcatccgt
     8821 aatatctatg tccagacaaa cggaacagca catagtggaa ttgctgtatt catggcattg
     8881 aagctgtatt tggactttat caacttgttc ttggcgtttc tgagaatctt tggacggaac
     8941 aactaaaaaa gaaaaaaatt catcagttgg ggctataccg aattatttgg tatggtcccg
     9001 ctttttcttt tcgcattccg caggattctt tgttagaatg aataagaact ttacagtaag
     9061 gagttaagaa gatgacaaaa aataaattat tactcgttga tgggaacagt gtggcttttc
     9121 gcgcattctt tgcgcttcac aattctcttg aacgtttcaa aaatagaaac ggtcttcata
     9181 caaatgcaat ctatgcattc aatacgatgt ttgaaaatgt tatgcaaaaa gagcaaccga
     9241 ctcatgttct ggttgcgttc gatgcaggaa aaacgacttt tcggacagaa atgtatgcgg
     9301 agtacaaagg tggaagatcc aaaacacctg gcgaattcaa agaacaaatg ccatatatac
     9361 gtgatttgtt gacaggacta ggggtacagt attacgaatt gcccaattat gaagcagatg
     9421 atattatcgg tactttggca gaaaaagtag acaaagatca atttgatgtc gtcgtgcttt
     9481 caggtgatcg ggatttgact caattagcta gtaaagaagt aaaagtggat atcactgtaa
     9541 aaggtgtcag cgatatcgaa agctatacac cagaacatgt agcggaaaaa tatgatgggt
     9601 tgacgccaaa acagattatt gacatgaagg gactagccgg agatgcctct gacaatatcc
     9661 ccggtgtgac taaaatcggt gaaaaaacag ctatcaagct attgaaacaa tatggctcag
     9721 tcgaaggaat ttatgaacat atcgatgaaa tgaagcaaag caaaatgaaa gaaaatctga
     9781 taaatgacaa agagcaggct ttcttatcga aaaaattggc gaccatcaat acttcagcgc
     9841 ctgtggatgt ctctatcgac tcattgaagt atgaagggaa aaatctcgat aaactcgttc
     9901 cgttttataa agaaatggat ttcaatcagt tcctttctaa attgaatatc gtagaagaag
     9961 aagtaaaaat ggatgatatt ttgtttgaag tcgttcatga attcaaagag gaaatgttta
    10021 ctacggatat ggcgttgtac gtggaaatga tgggcgacaa ttatcataca gaggaaatcg
    10081 tcggagtagc gtgggggaca gaaaagaaaa tttatgtcac gaacgatcta tctgtattcg
    10141 aaaaccgagc gttccattct tggatcactg atccgactcg gctgaaaaaa gtatatgatg
    10201 cgaaacgaac ttatgttgct ttaaaccgct atactggaaa aacgaaagga atcgattttg
    10261 atgtcttgct aggtgcatac cttctcgata cgaatgaaaa aagcacagat atcgaaggaa
    10321 tagctgctca ttacggatac aacgatatcc aatcagatga agctgtatac ggcaaagggg
    10381 cgaaaaaagg tttaccagaa gaagaggaac tcttttttgc tcatttagcg cgaaaagtag
    10441 ctgcaatcaa tgcgttgaca ccaaaattga gccaagaact ggcagataaa aaccaagaag
    10501 atcttttccg gaaaatggaa ctgccattgt ctatcatttt ggcagagatg gaaataagcg
    10561 ggatcaaagt agatgcgact cgcttacaag aaatgaaggg cgagttctct gctcgcttaa
    10621 gagagatcga acaaaaaatc tacgaagaag ccggcgaaga attcaatttg aattcaccaa
    10681 agcaattagg agtgatctta tttgaaaaaa tgggattacc cgttatcaaa aaaacaaaaa
    10741 ctggatattc cacagccgta gatgtactgg aacagcttcg tgaacaagca ccaatcgtag
    10801 aggatatcct cacctatcgt caaattgcaa aaattcaatc tacctatgtc gagggactgc
    10861 tgaaagtgat ccaatcagat ggaaaagtgc atacccgtta tgtccagaca ttgacccaaa
    10921 caggacgatt aagctctgtt gatcctaatt tgcagaacat cccgattcga ttagatgaag
    10981 ggagaaagat acgtcaggca tttgttccta gagaaaaaga ctggctgatt tattcttctg
    11041 actattcaca aatcgagtta cgcgtacttg cgcatatctc aaatgatgaa catttgaaag
    11101 ctgccttcct agaaggtcaa gatatccatg ccagcacagc gatgcgtgta tttggaatcg
    11161 aaaaggcaga ggatgtcaca ccaaatatgc gtaggcaggc aaaagcagtg aattttggta
    11221 tcgtgtatgg catctctgat tacggacttt cacaaaatct aggaatcagc cgaaaagcag
    11281 cacagcaata catcgatacg tactttgaaa aatacccagg tgtcaaagaa tatatggaaa
    11341 gaatcgtacg cgaagcaaaa gaccaaggct acgtcgaaac gctgtatcac cgccgtcgat
    11401 atctgccaga catcaattca agaaattaca atatccgttc ttttgcggaa cgaacagcta
    11461 tcaatacacc aatccaagga agtgctgctg atattttgaa aatcgcgatg atcgaattgg
    11521 ataaacgatt aaaagaaaca ggtcttcaag ctacgatgct gttacaagtt catgatgaac
    11581 ttgtcttcga ggtaccggaa aaagaacttg aatcattaga taaattggtg aaggaagtta
    11641 tggaacaagc tgtttctctt catgtaccac tgatcactga cagcagttgg ggaaaaacgt
    11701 ggtacgaagc aaaataaaca ttgtttaaga cagagcaata taatgaaggt gaaaagaaaa
    11761 aatgccagaa ttaccagaag tagaaaccgt tcgtaaagga ttggaacggt tagtagtagg
    11821 aaaaacgatt caaaaagttc aggtcttgtg gcccaagatc atcgagcaac cagagacacc
    11881 catctttgag gcttctcttg ttggcgaaac catccaatcc attggacgta gagggaagtt
    11941 tttaattttt catctcgatc attgtgaact gatctcacac ttgcgaatgg aaggaaaata
    12001 tcaatttacg aaagaaaata caccaatcga taaacatacg catgtacttt tcttttttga
    12061 ggatggaagc caacttcgct acaacgatgt acgaaaattt ggtcgtatga caatcgtcga
    12121 aaaaggagca agtgcaactt accgaggaat catgaaattg ggacctgagc ctttgccaga
    12181 tagcttctta ttagcagatt tcgcaaatgg actgaaaaag tcccacaaag caatcaagcc
    12241 attgctgcta gaccaacgtc tagtgactgg tctaggtaat atttatgtag atgaagcatt
    12301 atgggaagcg aagatccatc cagaacaacc ggcgaacaca ctgcgcccca aagaggtaga
    12361 acaattacgt ctatcgatca ttgatgtttt ggatcgtgca atagaagccg gaggtaccac
    12421 gattcgcagt tatttgaatg cattaggaga atcaggcgga tttcaagtag cactgcatgt
    12481 ctatcagcaa actggcaaac catgtatacg ttgtggaacg ccgatcgtga aaacaaaagt
    12541 ggcacaaagg gggacgcact attgtccgaa ttgtcaaagg ctaaaaatcg caaagtagga
    12601 tttgttttag gtttgacggg tggaatagct accggtaaaa gtacggcagc caaagtcttt
    12661 cagtctcatg gatttccttt gatcgatggc gatgtgattg ccagagaaac tgttgagcct
    12721 ggaacgcttg ctctgaggaa aattgtatca gtgtttggcc aagaggtatt gcagcctgat
    12781 gggcaactag atcgagggaa acttgggatg attgtttttc catcaaagga aaaacggcaa
    12841 aaacttgatc agctattaga tccgtttatc cgaaaagcga taaaagatca aatcgcctct
    12901 ctaagttcca atcatccttt agtaattgta gatatcccat tgctctacga aggtcattat
    12961 gaccactata tggatgcagt agctgtcgtt tatactacgc cagaaactca gcttcagcgt
    13021 ttgatgaaac gtaatcaact gacgctagaa caagcgcagc agagaattag tagtcaactt
    13081 ccaatcgaag aaaaaaagca gcgtgcggat attttgttcg acaacaatgg aacaaaagaa
    13141 aaacttgtgg gacaaatcga aaattggctt tttgctaatc gctttctttg agtatctgtg
    13201 tcaattttgc tatcctgagt atatagaaag tgagggatag cgatgggatc attaaaaaat
    13261 caaaaggata aagtcgtagg taacgcaaaa gaaaaaatcg gcgagatgct caataaagaa
    13321 aatctaatcg atgaaggtcg tacgcaatca gaaacagcag aagaaaaaga aaagcagtac
    13381 caagcaagaa tcgatgaaat agaagaccag caggcagaag aagaagcgaa gcggcaagat
    13441 ttgactcaag aacgaaaaaa tccgacacct gatcgtagta aaacaccgct cccagaaagt
    13501 gaccacattt ccgatgagca aaaaatcgag gaacagcgaa tgaaacacta tacaggaatc
    13561 gaagagaagt tataaaatag taaaaaatcg agatgtgact tgaagcagat gagaaggtgt
    13621 tcatctgctt caagtttttt gtgctagatg actaaatcag cttttgtgta agttatttct
    13681 cagtaatcaa ggtgatcagt attggaaaat tgaaatttag taaggacagg ggacacaatt
    13741 acgcatgaaa gtagaaaaaa ttgttcttat atccgtgttt tttgggtagg acagtcgcgt
    13801 ttgtgatttg tttcgtgtta taatacaact agttgtaaag aaagacacat cctttatata
    13861 aaatgcgatg caagaggtga gacaaaatga gatgtcccag atgtcaacat aataattcac
    13921 gagtaatcga cagccgtcaa gcagatgatg gacgtgcgat ccgtcgccgt cgcgaatgtg
    13981 agaattgttc gttccgattc actacatttg aacgaattga agctgctccg ttattagtaa
    14041 tcaagaaaaa cggggaacgc gaagagttca atcgcgataa aattttaaga ggactcatcc
    14101 gttcagcaga aaaaagacct gtagctatgg aacaaatgga acaaatcgtc aaccatgtcg
    14161 aaaaccgcgt acgcagtctc ggagaaaacg aagtatcgac gaatctaatt ggtgaatatg
    14221 tgatggaaga tttagttgat ttagacgaaa tcgcttatat tcgttttgca agtgtttatc
    14281 gccagttcaa agatatgtct gttttcttaa aagagttgca agatatcgtg gataaagcaa
    14341 aagaaaataa taaagagaat aataaagaaa acaattaaga aacaaaaagg aggcatttcc
    14401 ttggaaaatg cttggaaaga gcttcaacca aaaaatattt accaaggaag aaaagcggct
    14461 ccgctgagag aagacagcaa gaacgccttg ctgtatcttt atcagccact aatcggcgga
    14521 gaagccttga gtatctattt caccctgtta tcagaaatct ctcaagaatc tggcaaaggt
    14581 cctgaaggga tgcatgcaga tctgcttagt agtctaggct gcggtatccc gcaattttat
    14641 gatgcaagaa aaaagctaga aggaatcggc ttactggatg tttattttaa agaagatagt
    14701 gatctgggac ctcgttttat ttatgagctg ttagaaccac tcattcctaa agcatttttc
    14761 aaagactctg ttctggcttt tcttcttctg gaaaaagttg gtgaacgacg attccagaag
    14821 cttacccaat tctttgaacc taaaaggcat acagcagagg gatatcaaaa aatcacaaaa
    14881 aaatttttag atgtttatcg cttcaatgaa gcagtgtacg ctgctaatca agaaaacatc
    14941 gaatcgctga ataaatcttt tgaagaatct gaatcagtag cattacttga agcagattcg
    15001 acattcgatt ggcagttttt gaatgattgg ctggcaaaaa aacatattgc aagtcttgat
    15061 gatccaatca tcaaacaagt cgacatgtat catcagcttt acggtttcga tgaattgaca
    15121 cttggcgaac tgatcagtca atcttatgac tttagtgaac aaaaagtatc tgaaaaagaa
    15181 ctgcaaaaag ccattttggc aatgcagaga cgtccggtga agcaaacaaa agaaacagtt
    15241 gcggtttctg aaaacagtaa tgaaccctcg tcaaacgaac atttgacaaa cgcagcgctt
    15301 cagctgataa aagaagcaca gagcattcca ccgatgaaat atctggaagc aatcaagtca
    15361 gaaaaaggcg gatatgtttc taaacaagaa aactggttac tgcaagatct tgtttctcaa
    15421 tcaggtcttt ccagtagcgt gatcaatgtt ttgctgaatt atgtactagt gatcaagaat
    15481 aatgctagct taaatgccag ttatgtaaat acagtggcaa atgaatgggc acaaaagaaa
    15541 atcgccacag cagaagaagc gatccagcac attcggcaaa taagtaatca agcaaaacaa
    15601 tcaaaacaaa agaagcaaac aaattatcaa actggcaagc gaaatgttcg acgggaaaag
    15661 cttcctgatt gggtcaacca gccaaaagat gaaaaacaga tcagcagcga gaaaaaagca
    15721 gagattgacc gccgttttaa agaatacctg gcaaaaaagg agggtgatat ctagtggaag
    15781 acgtaggcaa agaattgaag aggatcatca gcaaacgcaa ttatcaagaa aagtatgctg
    15841 aaatgatcgc tgaagtctta aatgatgcgg atgttcaagc ttttctaagc gcacaccaag
    15901 aagaattgac acaagcggat attgaaaaaa gttacgctaa attgtatgaa ttcgtgcaag
    15961 aaaagcgtaa atatcaaaaa aacgatccaa cgatgatcgc tcccggctat gagccgaagc
    16021 tgacgttgaa ttttcattat gtggatgtca cttatgtacc aacggaagca ttgttagcac
    16081 ggcaaagaca agaagaaatc agaagccgag taaaagcaat ggatatgcca aaagatattc
    16141 aatcggcttc ttttgaaaac tttgaacgta cagatggacg aggatatgcg agcttagaag
    16201 ccctagattt tgtagagaaa tatactgctg atccaaaagg gtatcataaa ggactgtatc
    16261 tagtaggaag ttttggtatt ggaaaaacat atttattagg agcaacagcg agagaattag
    16321 cgattgctgg attcaccacg acgctagtcc attttccgac atttgccgtt gagatgaaac
    16381 aggcaatcgg aaaagatcaa gtggcagaaa agttagatgc agtgaaacgt tcacctattt
    16441 tgatgatcga tgacattggt gcagatgcga tgagtagttg gatacgagac gaagtattcg
    16501 gtgttatcct tcaatatcgg atgcaggaac agttaccaac cttcttctca tcgaatttta
    16561 caatggatga attggaacag catttgtccg tcactcaacg tggagatgaa gagccgctaa
    16621 aagcgaaacg gataatggaa cgcatacgtt atttgacaaa agaaatcgaa atgactggtc
    16681 gcaaccgacg caatggttga acagttgaca agtccaactg acatttgacc tagtagtaaa
    16741 agcaggtcag cgaatcatta tatggacaga aatgaccgga gacaatcacc gcaacaagta
    16801 atgcagaaac ttaaacggag gattttttat gagggctatt ttttctagta tatctgtagt
    16861 tggtctattt tttcttttag caggctgcgg tagcgcagat agcaacacag gttctgttga
    16921 ttcgattgat gcgggtcaaa cagtaaaaac aagcagtacg aaaacggaga gacaaagcat
    16981 atttgtcgga acgattgaaa gtattactca agtagaagca gaagaagaaa cgcttcagtt
    17041 gaaactcgtc gatgtcatgg ccaaagaaga tccagcgaat atgggaacgt cattcagcaa
    17101 tgacggagtg atcttaaatg ctactgctga aaaaataagc gtggacataa atgaattgaa
    17161 aatcggtgac aaagtccaat ttacattagt tgagatgccg atcatgacga tgtctatccc
    17221 accgcaagtt cctggagatt cgattattga tgtttcattg aacaaataac caataacatt
    17281 ttttcaaaaa taagaaaatg ttgttttata gaacgaaaag gtatattttt taaggagata
    17341 tggg
//
LOCUS       DACDKM010000051        17326 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20834_2016
            SAMN12640370-rid9891663.denovo.051, whole genome shotgun sequence.
ACCESSION   DACDKM010000051 DACDKM010000000
VERSION     DACDKM010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640370
            Sequence Read Archive: SRR10040891
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17326)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17326)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742835.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742835.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:04:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,012
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 29 of 75
            Pseudo Genes (incomplete)         :: 33 of 75
            Pseudo Genes (internal stop)      :: 19 of 75
            Pseudo Genes (multiple problems)  :: 5 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17326
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640370-rid9891663.denovo.051"
                     /isolate="E20834_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            129..566
                     /locus_tag="IV334_001594"
     CDS             129..566
                     /locus_tag="IV334_001594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8943666.1"
                     /translation="MEWFCKFLLFLEVIHVYGFVFLTNKKAGSTMDHLYIKYIHQFSV
                     LVLGTLIIAGIVAFIIQGRDILTVHQEFFSYAFGIPLIAIRSDNKKWKKRYYILLLFL
                     CFYLPPKIFTLLGIQCLSFSFISYIIVLIFLSFIVWMFYLVKK"
     gene            661..1545
                     /locus_tag="IV334_001595"
     CDS             661..1545
                     /locus_tag="IV334_001595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family lipid kinase"
                     /protein_id="HAP8943667.1"
                     /translation="MKKVLLVVNPSSGGEQAKEFEQLAIAKLESVFDEVVVLHTKKAG
                     DAKNFTREAATEGYHSVFVMGGDGTVNEGISGIAEQEHRPNFGFFPLGTVNDLARALG
                     IPLEPEEAINHFSIESVKPLDIGKINDDYFMNVVAIGSIPESINDVDAEKKTKFGKLA
                     YFMSGIKQLASTQSYSFHVEVDGKKEEIESSTLLIGLTNSVGGFETLLPNAKVDDGKL
                     HLVYLKDSSLIDTLKTLPDLLKGVDESTKNLAYLTCEQLVVSLADEEELTTNVDGDEG
                     DKLPVTIKVLPSHLQVYC"
     gene            1769..4936
                     /locus_tag="IV334_001596"
     CDS             1769..4936
                     /locus_tag="IV334_001596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8943668.1"
                     /translation="MNQCVKKLIASILFVFPLLMVTSVFADVEKTNESTQSENQSFDK
                     TTFSEESITSETTESQSEASTNKRQEENKTSETSEVAEQTSDSSEAALARAVSADGYS
                     EAATTEELAQLLADESVTKIRLIQPLTLDRELEIKRDIVIDFGGFAHNFGTHHIYINE
                     IPQSIEFQNFKGTAAHPGGLTPNIDGNAIIIAYMTDFWGSRYHFTGEIKFTGTLDLYD
                     GSKLGLIYAPRATVTLDGVSGVLDVQPVKEGLNAAPGKAYFCRSYQLNVINGSQLYGP
                     YLGKFYGFLDSGDETGSGKTAPGIHILSGSKVSLDYDRADGVSDEGEAVDTLPGNVMF
                     EVSGSGSEFSVNTKINITNDNNRGIIQMRGSGSKVTVSNGGKITINTATTGGFRLQGD
                     SSQIHVSSGGQISVKMAGDDDQPGNNGMRFVGKGLSLIVEGAGSVIDIDKQSGRSSAV
                     RFENGTQKLTVTNGGSLRVRNTGDGKSYVNRLNQGNQAIQFYDASGFLESPGSADFTV
                     TGEKSNIDIRADSGATVDTTGDIDLNFTAGEKTYLVMVGKTGEDKTGIISGDVLNVDL
                     ESPTYFDFQNKRIGSETNGIGGWVFQGNGDSSLKIAHSEIALWRKTGATFDNFEKDPD
                     YFSDMTDLSINGTDLGNFVSSPSAGLTNEFAQTGAGMKNYNRISANNQLPMIESLRVP
                     TNADKKIYGHVVVPEGVEAKPRDAWTGEVEVTLRITYADTTKSSVEITALTQGQTDEN
                     EGAGYNPWGEGEQGGLFEVQVPNDERLKTEDQVQVVAAKKVRGGQEVTELDSAKTTVD
                     VIPPEPANLSTTLINAASRTITGTGEAGALVSLKKNQAVLSETTIDDTGEFVLSIPEN
                     QLFAGDVLEIVLNDQAGEATAKGVQNKPSTNDEIGNHNPSDTPVVYHDAPPFAPATKI
                     VVEGGLSFLAPSKLDFGQIKATGLTQEAFGQMDIQEKLWIYDQRETKNPWILTIKLSQ
                     PFTNDAGFSMQDVLYFKKGEEYQLVNEEELEIVRGQNSTNVQADYSEYLNNGRAFKLV
                     LTKENQIAGNYSAEITWTLADAPTE"
     gene            4956..5501
                     /locus_tag="IV334_001597"
     CDS             4956..5501
                     /locus_tag="IV334_001597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014386985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 domain-containing protein"
                     /protein_id="HAP8943669.1"
                     /translation="MNRIWNKSHRNNCSLLMILLLGFILWQTPVHASEFNFAVDFELP
                     ANQIDKNKTYINVRMEPGKTQEIPYTVFNRTDKSVTVETLIHSATTNSNGVIEYGESK
                     SKFDETLSYDMKNLAKTVGEITVPPQSAVTETLSLKAPEKELAGVVAGGITFQEKKEN
                     VSNKSTVGVVNQFSQVKAILI"
     gene            5529..5984
                     /locus_tag="IV334_001598"
     CDS             5529..5984
                     /locus_tag="IV334_001598"
                     /inference="COORDINATES: protein motif:HMM:NF023225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3324 domain-containing protein"
                     /protein_id="HAP8943670.1"
                     /translation="MKLKKAYADQSDGRNVIKANLQNPNASYMFGLTTSVEIYKKETK
                     EPYLSIKKEKIDVAPNTNFDLLVPLEGKKLAPGHYQAVVKGAWKEKRWNLHTDFEITD
                     DAADQLNEKDIDLKNQPETFDWRWLILVGVILIGLFAGLFILKERRKKK"
     gene            5981..6739
                     /locus_tag="IV334_001599"
     CDS             5981..6739
                     /locus_tag="IV334_001599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HAP8943671.1"
                     /translation="MKKTIALSTVLAGSLLLCTQSVFAENKSSATTEGTISVTKPDAA
                     NPEDVVPVPPVDPNDPDTPFTPDEPNQGTSGLLTIDQAPDLDFGTIKLGTAKTVYAAL
                     QSGTDSTGAQTQVQNFVQVTDKSGNYAGWKLSVKRTEFIGTTSDTNKLTGSKVKFKNA
                     VIRNSATNSSGMPSTVSGTGAEGIEIPADQAVDLVTAGANEGIGTWIYSLGENVEQGR
                     QSVELFVPVSNYAVDNYVSTFTWSLTDAPTEEEA"
     gene            complement(6825..7565)
                     /locus_tag="IV334_001600"
     CDS             complement(6825..7565)
                     /locus_tag="IV334_001600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP8943672.1"
                     /translation="MKKYFIIEDNQFHSEWIIRELVNHHIENIVQLTSISQINQLIQL
                     GTIDNHDVFFIDIDLKQSLTGIDVAEQIRKKNAHSNIVFFTAYTNFAVEIINRKILPI
                     GYIRKDRNLSEQIRQVLEQINWKEQQIVNVDKIFIDQKGGSLVLYPREILYIESIKGL
                     KNQVMVKTTTEDKLVNTSLKQLKTELEKYAYFLLLKSYCINLSQLQKIDSAHYVLEFF
                     NGDSLLVGRKIFEKTKERFHDFQKTASS"
     gene            7626..8879
                     /locus_tag="IV334_001601"
     CDS             7626..8879
                     /locus_tag="IV334_001601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HAP8943673.1"
                     /translation="MVATVTLYLTFTLILDLGIYLFFSKQLLVKNSFGCIALMVVTLL
                     LPIPKVSFPYLVGLLLMFLFYFTTKNLFISCLISNLFFAITGLSFFLVFDLMDYLGKR
                     HLGTASFFALLIAFIFYLLVKSIDKQTRFLNLLFTYPTEHYFGGICILLSWGVIYLVL
                     GSVHFRSSNYLLLSLLSIVISFFSLFFSIMLISFQVKERQHQESLQLYQINEEYYHHL
                     EEFKHDYKSLLFSLKITLQENKKETLEFLEILEDHSAAIFEKPQIQQLTNIVSPAVRG
                     VFLKFLEKAESEKIPIQVVIPVEVKTIPIDLVVFIRCLSIFISNAFDHRVADQSPITI
                     NLLEEKEGSRFSIANKANPTNLTLSEMVKRGKTSKKNGGLGLYSAKKMLDVYENAELK
                     IEFSKQNHYFFVEMYLASRKSKSAY"
     gene            9201..9428
                     /locus_tag="IV334_001602"
     CDS             9201..9428
                     /locus_tag="IV334_001602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HAP8943674.1"
                     /translation="MLTQQLRELEQDESIHRKAYNQVPPKVEYSLTEEGKSLREILVA
                     LSVWEEERIEKQKASREEDTLLHTHGGYLNY"
     gene            9551..9877
                     /locus_tag="IV334_001603"
     CDS             9551..9877
                     /locus_tag="IV334_001603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HAP8943675.1"
                     /translation="MNSMEKVNQFRDERNWRQFHNEKDLAISISLEASELLELFQWKT
                     PEEVKETQLERIKEELADVLIYSYMMADNLHLDLDEIIEEKLIKNNLKYPVEKSAGVR
                     KKYTEL"
     gene            9945..10658
                     /locus_tag="IV334_001604"
     CDS             9945..10658
                     /locus_tag="IV334_001604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A sortase"
                     /protein_id="HAP8943676.1"
                     /translation="MKKKGKAKRWLVNILLFLLLLVGLALIFNEQIKDYFVRETGDKY
                     AIANVTKEDLKKNNDKDVSFDFDAVEPMTTEGVMRSQMRGTDLPVIASIAVPSVSINL
                     PVFKGLDNTSLLYGAGTLSPDQEMGKGNYALASHRATNPELLFTPLENLEMGAKIYLT
                     DLENVYTYKTFFKEKVAPTDTQLLNEVEGKEIVTLITCGDMDAVTRLVVQGELESVTS
                     IKDATDDMRSAFNLETKTF"
     gene            10968..11819
                     /locus_tag="IV334_001605"
     CDS             10968..11819
                     /locus_tag="IV334_001605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAP8943677.1"
                     /translation="MKIDKILNNNVVISKNGFGEEVVCMGKGLAFQKKIGDEISPEAV
                     QKEFVLRDSFAKNQFQQLMADIPAEEIEWVKQVVELAEDKLKVEFSPNIYLTLTDHLH
                     YAITRAKENIQLPNPLLFETKKFYPKEYQAAKEALLFVKQKTGVELSEDEAGFIAFHF
                     VNSQQGNEDMQVTMTATTMVRDILSIISKFFGIVFDEESLNYQRIITHLQFFAQRYLK
                     GEGSDEQDEFLYALVQGKYPKAFHCVERINEYLRQTQKQEMGIAEQIYLTIHIQRVVS
                     EKKSIQS"
     gene            11940..13805
                     /locus_tag="IV334_001606"
     CDS             11940..13805
                     /locus_tag="IV334_001606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP8943678.1"
                     /translation="MDNQAVGRRVWEAVGGQKNVKSLVHCATRLRFRLKDESLADTQK
                     LKEDPDVIQVVQSGGQYQVVIGSNVADVYQSIVDEEGLAVSEDGKEESKNILNRLIDI
                     ISSIFTPFLGAMAAAGVLKGFLSLATVMGWLTADSGTYQILFAAADGVFTFLPVMLAF
                     TAAKKFKANQFLAVAIAMALVYPAITAVAGAGEAISFFGIPVILSPSGYTSSVIPIIL
                     AVWVQSKLEPFVKKVIPQFLQMILVPLVVLVVMVPLTFLALGPIGTVAGNALGGLFNS
                     IYGFSPIVAGLIMGSLWQVFVMFGMHWGFVPIMFLNIEQYGFDVLMPMLLPAILAQGG
                     AALAVALRTKDTKLRALGISSTVTSLFGITEPTVYGVTLPLKKPFIAACISGGIGGAI
                     IGFSGVKAFSSSLVSLLTIPTFINTVDGVESNVTVAVIATGIAFVLAFVGTLILGFDE
                     QTQDNQLENKHANAGKPITSARHTLKSPLTGKVLPLSEVPDQVFSSGVMGKGIAIDPE
                     VGELVAPADGEITTIFPTGHAVGITTTDGAEILIHIGMDTVELNGNGFEILVKQGDLV
                     KAGDLLIRFDIEAIRAAGYSVITPVVITNTDAFADILELDQKEIIANEDVLAIVK"
     gene            13853..15262
                     /locus_tag="IV334_001607"
     CDS             13853..15262
                     /locus_tag="IV334_001607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 1 glycosylhydrolase"
                     /protein_id="HAP8943679.1"
                     /translation="MEQLLPNQLEGAYDRDGKGLSVADAMPGGKQRFAIIGSEEFDWT
                     IDQEKYIYPNHRGIDHYDRFKEDLALFAEMGFKCYRFSIAWTRIFPNGDEGTPNEAGL
                     EFYDQLIDECLKYDIEPVITISHYEMPLHLAKEYGGWKNRKLIDFYERFAQTVLERYS
                     SKVKYWMTFNEINSAFHFPALSQGLVKSNGAGEYQNIFQAWHNQFVASSKAVKIGHEL
                     RSDIQIGCMIIYATTYSIDANPVNQAATMIQNQEFNFFCTDVQVRGEYPAYTARTHKK
                     YGVDPEKLEQTEKDFQLLKEYPVDYIGFSYYMSTAINETDPQAATSEGNLLGGVKNPF
                     LEASEWGWQIDPEGLRIALNELYNRYQKPLFIVENGLGAIDHMEEDGTIIDDYRIDYL
                     RRHIEAMANAVADGVDLMGYTPWGCIDLVSASTGEMSKRYGFIYVDLDDEGNGTLNRS
                     KKKSFDWYKEVIASNGVNL"
     gene            15370..16071
                     /locus_tag="IV334_001608"
     CDS             15370..16071
                     /locus_tag="IV334_001608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAP8943680.1"
                     /translation="MKRTRVLYLEVADKIKEDIFSGKYPVGSMLPTESELEELFQVSK
                     ITVRKAIELLASDEYVAKKSGRGTTVLSNRPYNRLSKAASFTQILENSRYEVRKETLD
                     LQLVHIKKEDPLFSFFGEQAVCFKRLYYLNNQPYIYFVHYLPQEMMQFSREEFGKESL
                     YRLLTKKGYTIDSFSDDFSAVFLTEEEQGILKTDAQIGIKRIRKSSTQIGEMIEYSEA
                     VYHTELHPYHIDYET"
     gene            16230..16709
                     /locus_tag="IV334_001609"
                     /pseudo
     CDS             16230..16709
                     /locus_tag="IV334_001609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015510350.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
BASE COUNT         5783 a         2908 c         3718 g         4917 t
ORIGIN      
        1 ttactacttg atttagttct ttttatttta aaaatttttt ttacttagaa tccagcgacc
       61 ctagatattt ttctgtataa tcttaaataa agaaaacaat atcaaaaaat gcacgtagaa
      121 gtagataaat ggagtggttt tgcaaatttc ttttatttct agaagttatt cacgtatacg
      181 gatttgtttt tctcacaaat aaaaaggcag gaagtacaat ggatcatttg tatataaaat
      241 atattcatca attttccgtt ttggtgttag gcacactaat tatagcgggg attgtcgcat
      301 ttattattca aggccgtgac atattaacag tacatcaaga gtttttcagt tatgcatttg
      361 gtattcctct gatcgctata aggtctgata acaaaaagtg gaagaaacgc tattacattc
      421 tcttgctatt tttgtgtttt tatctgccac ctaagatttt tactttatta ggtatacaat
      481 gcctatcatt ttcttttatt tcctatatca ttgttttaat tttcctcagt ttcatagtat
      541 ggatgtttta tcttgtcaaa aaataaatga atgtttcatg aacagtactt catcacagcc
      601 taattttttt ctttatgcta tcgttatatt aaagaaatca atcagaaggg aaagaaagtg
      661 atgaaaaaag ttttattagt ggtaaacccc agttcaggcg gtgaacaagc aaaggaattt
      721 gagcaattag cgatagcaaa attggaatcg gtgtttgatg aagtggtcgt cttgcataca
      781 aaaaaagcag gggatgcaaa aaactttact cgcgaagcgg ctacggaagg gtatcatagt
      841 gtgtttgtca tgggcggaga tggaacagtc aacgaaggaa tcagtggaat cgctgaacaa
      901 gaacatcggc caaatttcgg atttttccca ttgggtacgg tgaatgactt ggctcgagca
      961 ttagggatac cgctagaacc tgaggaagcg atcaatcatt tttcgattga atcagtcaag
     1021 ccattggata ttggaaaaat aaatgacgac tattttatga atgtcgtcgc aattggctct
     1081 atccctgaat caatcaatga tgtagatgcg gagaagaaga cgaaatttgg aaaactagct
     1141 tatttcatgt caggaatcaa acagttagct tccacacaat cctactcgtt tcacgtagaa
     1201 gttgatggaa agaaggaaga aattgagagc agtaccttgc tgataggact aactaactca
     1261 gtcggcggtt ttgaaacatt acttccaaat gcaaaagtgg atgatggtaa gctccatttg
     1321 gtttatttaa aagattcttc tttgatcgat acgcttaaaa cattgccaga cctattaaaa
     1381 ggagtagatg aatctacgaa aaatctagcc taccttactt gtgagcaatt agttgtgtca
     1441 ttagctgatg aggaagaatt gacgacgaat gttgatggag atgaagggga taaattacct
     1501 gtgacaatca aagtattgcc ctcacattta caggtttatt gttaaaatgg aggttgtgac
     1561 cgaggagttg gcgggagaac accgcttgtt cgggataagg gactgtgaca agacttttgt
     1621 cacagccttt tttttgtaca aaaaagaaat ttacacctaa tagaaaaatt tttccataaa
     1681 aatacatatt ttaaatatga aagtacaatt ggaaatagat taaaacgata aaataaaatt
     1741 gtcaaaacgt cagagaggat ggtggagaat gaatcaatgt gtaaaaaagc tgattgcgtc
     1801 gattttgttt gtcttccctc tcttgatggt gacatccgtt tttgccgatg tagagaaaac
     1861 aaacgaatca acgcaatcag agaatcaaag ttttgacaag actacttttt cagaagaatc
     1921 gattacttct gaaactacag aatctcaaag tgaggcttct actaataaaa ggcaagaaga
     1981 aaataagaca agtgagacct cagaagtagc tgaacaaacg tctgatagca gtgaagcggc
     2041 actggcaaga gctgtttcag cggatgggta cagcgaagct gccacaacag aagaactggc
     2101 gcagcttctc gcagacgaat cagtaactaa gatccgactg attcagcctt tgactttaga
     2161 cagggaatta gagatcaaac gagatatcgt tattgatttt ggtggattcg ctcacaattt
     2221 cggcacacac catatttata tcaatgagat acctcagtcg atcgagttcc agaattttaa
     2281 aggaacagct gcacatccag gtggattgac accaaatatc gatggaaatg ccattatcat
     2341 tgcttatatg accgattttt ggggaagcag atatcatttt acgggagaaa taaaatttac
     2401 cgggacactt gatttgtatg atgggtccaa attaggactg atttacgctc ctcgtgcaac
     2461 tgtcacatta gatggtgttt ccggtgtgtt ggatgtccaa ccagtgaagg aaggactgaa
     2521 tgccgctccg ggtaaagcct atttttgtag aagctatcaa ttgaatgtga tcaatggtag
     2581 tcaactttat ggaccgtatc tagggaaatt ctacggtttt ctagattcag gagatgagac
     2641 tggtagtgga aaaacagcgc caggaatcca tatcttaagc ggttcaaagg tctctcttga
     2701 ttatgatcgg gcagatggcg ttagcgatga aggagaggca gtcgatacgc tacctggtaa
     2761 tgtcatgttt gaagtcagtg gttcgggaag cgaattttcg gtaaatacaa agatcaatat
     2821 taccaatgac aacaatcgcg gaatcatcca aatgagaggt tcaggatcga aagtcactgt
     2881 atcgaatggt gggaaaatca ccatcaatac agcaactacg ggcggatttc gcttgcaagg
     2941 agattctagt caaatccatg tatcaagtgg tggacaaatc tcggtcaaaa tggctggaga
     3001 tgacgatcaa cccgggaaca atgggatgcg ttttgtagga aaaggccttt ctttgatcgt
     3061 agaaggagcc ggatcagtta tcgacatcga taagcagagt gggcgttcca gcgcagttcg
     3121 ttttgagaat ggtacgcaaa agctgacagt aaccaatgga ggctcattga gggtaagaaa
     3181 tacaggtgat ggcaaatctt acgtgaacag actcaatcaa ggaaatcaag cgatccaatt
     3241 ttatgacgct tctggttttc tagaaagtcc aggttcagct gattttacag tcactggaga
     3301 aaaatcaaat atcgatatac gcgcagattc cggagcaact gtagatacaa ctggagatat
     3361 tgatttaaat tttacggcag gtgaaaaaac ttatttagtc atggtcggga aaacagggga
     3421 agataagacc ggcatcatca gtggggatgt gttgaatgtg gacttagaat ctcctaccta
     3481 ttttgatttc caaaacaaac ggattggaag cgagaccaat ggcataggcg gctgggtatt
     3541 ccaaggaaat ggagattctt ctttaaagat cgcccattca gaaatcgctt tatggcgaaa
     3601 aacaggcgca acattcgata actttgaaaa agacccggat tatttttctg atatgacaga
     3661 cttatcgatc aatgggaccg acttaggcaa tttcgtatca agccctagtg caggtttaac
     3721 aaatgaattt gctcagacag gtgccggaat gaaaaattat aatcgaatca gtgccaataa
     3781 tcagctcccg atgattgaat ccttaagagt gccaacgaat gcagataaaa aaatctatgg
     3841 gcatgtcgtt gttcccgaag gtgtcgaggc aaagccaaga gatgcttgga caggcgaagt
     3901 agaagtgact ttacgtatta cttacgcaga tacgacgaaa tcatctgtag aaattactgc
     3961 tctcacgcaa ggacaaacag atgaaaatga aggggcagga tataacccat ggggagaagg
     4021 cgaacaaggc ggcttgttcg aagttcaagt acccaatgat gagagattaa aaacggagga
     4081 tcaagtgcaa gttgttgctg ctaaaaaagt aagaggtggc caagaagtga cagaactgga
     4141 ctctgccaaa acgacagtcg atgtgattcc tcctgaacct gctaatttaa gtaccacatt
     4201 gatcaatgca gcttctcgca cgatcacagg tacaggtgaa gcaggagcct tggtttcatt
     4261 gaaaaaaaat caagctgttt tgtcagaaac aactattgat gatacagggg aatttgttct
     4321 ttctatccca gaaaatcaac tttttgcagg agatgtatta gaaattgtat taaacgacca
     4381 agcaggagaa gctactgcta agggtgtgca gaacaagcca agtaccaacg atgaaattgg
     4441 gaatcacaat ccaagtgata cgccagttgt ttatcatgat gccccgcctt ttgcacctgc
     4501 tacgaagatc gtagtagaag gtggtctctc atttcttgcc ccttccaaac tcgattttgg
     4561 tcagatcaaa gcaactggtt tgacacaaga agcatttggc caaatggata tacaggaaaa
     4621 attatggatt tatgatcagc gagaaacgaa aaatccgtgg atcttaacga taaaattatc
     4681 tcagcctttc acaaatgatg caggattttc tatgcaggat gtcctatatt tcaagaaagg
     4741 agaggagtat caattagtga atgaagaaga actggaaatt gtcagaggac aaaattcaac
     4801 gaatgtgcaa gcagattatt cagaatattt aaataacggt cgggcgttta aacttgtctt
     4861 aaccaaagaa aatcagatag ctggtaatta ctcagcagag ataacatgga cattagcaga
     4921 tgcgccgaca gaataaagat caaggaggat gattgatgaa tcgaatatgg aataaaagtc
     4981 atcgaaacaa ctgctcttta cttatgatcc ttctccttgg attcatcctt tggcagacac
     5041 ctgtccatgc ctccgaattc aattttgcgg tagattttga attaccggct aatcagattg
     5101 ataagaacaa aacatatata aatgtaagga tggagcctgg gaaaacacag gaaatcccct
     5161 acacggtctt taaccgaaca gataaatcag taacagttga gacattgatc cactccgcta
     5221 cgacgaattc aaacggtgtg atcgaatatg gagaatcaaa aagtaaattt gatgaaacat
     5281 taagttatga tatgaaaaac ttagcaaaaa cagtgggaga aataactgtt cctcctcaaa
     5341 gtgcggtcac tgagacactt tcactgaaag cgccagaaaa agagctggct ggtgtggtcg
     5401 caggagggat cacgtttcaa gaaaaaaaag agaacgtttc taacaaaagt acagttggtg
     5461 tcgtcaatca gttttctcaa gtgaaagcaa tcttgatttg aaatagcgaa aaagagtctt
     5521 ccccagattt gaaattaaaa aaagcttatg cggatcaaag cgatggaaga aatgtgatta
     5581 aggcgaatct tcaaaatcca aatgcttctt atatgttcgg cttgactaca tcggttgaaa
     5641 tctacaaaaa agaaacaaaa gagccttacc tttcaataaa aaaagaaaaa atagatgtag
     5701 cacccaatac aaattttgat ttacttgtcc cattagaagg aaaaaaatta gctccgggac
     5761 attatcaagc ggttgtgaag ggtgcttgga aagaaaaacg ctggaatctc cataccgatt
     5821 ttgaaataac tgatgatgca gcggaccaat tgaatgaaaa ggatattgat ttaaaaaatc
     5881 agccagaaac atttgactgg cgatggctga tacttgttgg agtcattctt attggcttat
     5941 ttgctggatt atttatttta aaagaaagaa ggaaaaagaa atgaaaaaaa caattgcatt
     6001 aagtaccgtt ctagctggaa gtctattatt atgtacacaa agtgtttttg ctgagaataa
     6061 gagctcagcc actacagaag gaacgatttc tgtgaccaaa ccagatgctg caaatcctga
     6121 agacgttgtt ccagttcctc ctgttgatcc aaatgatcct gatactcctt ttacacctga
     6181 cgaaccaaat cagggaacat ccggattact gacgattgac caagctccag atcttgattt
     6241 tggaacaatc aagttaggta cagcaaaaac agtatatgct gcattacaaa gcggaacgga
     6301 cagtactgga gcacaaacac aagtacagaa tttcgtacaa gtaacggata aaagcggaaa
     6361 ttatgccggt tggaaattat cagtcaaacg aacagaattt attggtacaa catctgatac
     6421 gaataaacta actggttcaa aggtgaaatt caaaaacgcc gtgattcgaa atagcgcaac
     6481 taatagcagt ggaatgccat ctaccgtttc tggaactggc gctgaaggaa tcgaaatccc
     6541 agcagatcag gcagtagatc tagtaactgc aggagcaaat gaagggattg gcacatggat
     6601 ttattcatta ggagagaatg tggaacaagg gagacaatca gtggaattat ttgttcctgt
     6661 atccaattat gctgtagaca actacgtttc aacattcaca tggtcgttaa cagatgctcc
     6721 tacagaagag gaagcgtaaa atcatatcaa cgatttcagg gagtggagcc gattcaaggc
     6781 ccactccctg aaatctgcta tgcttacgtc tcagctctta ttcatcaact cgaagcagtt
     6841 ttttggaagt catggaaacg ttcctttgtc ttttcaaaga tctttcgacc aacgagtaag
     6901 gagtcaccgt tgaaaaattc taacacataa tgagcagaat caattttctg caattgcgat
     6961 aagttgatac agtaagattt taaaagtaaa aagtaagcat acttttctaa ctctgtcttc
     7021 aattgtttta aagaagtatt cactaattta tcttctgtag ttgttttgac cataacttgg
     7081 tttttcaaac ctttgatact ttcaatatat aaaatttccc gaggatacag gacgagactt
     7141 ccaccttttt gatcaataaa gattttatcg acatttacga tctgctgttc tttccaattg
     7201 atctgttcta gcacttgacg aatttgttca gataaattcc tgtcttttct gatatatcca
     7261 atcggaagga ttttccgatt gatgatctct actgcaaaat tcgtataagc ggtgaaaaaa
     7321 acaatattac tatgtgcatt ttttttacgt atttgttcgg cgacatcaat acctgtgagt
     7381 gattgcttca agtcgatatc gataaagaaa acatcgtgat tatcaatagt gcccaactgg
     7441 atcaactgat tgatttgaga gatagaagtg agctgcacga tattctcgat atgatgattg
     7501 accaattctc tgatgatcca ttcgctgtga aactggttat cttctatgat aaaatacttt
     7561 ttcatatgca ggctcctatt aaaaaaatat gtgtacattt tgaaacaaaa caatggagga
     7621 ataagatggt cgcaacagtg acactttatt tgacctttac tttgatttta gatttaggta
     7681 tctatttatt cttttcgaaa caactgctag taaaaaacag ctttggttgt atagctttga
     7741 tggtggtgac acttttactt cctatcccta aagtgagctt tccttaccta gttggcttat
     7801 tattgatgtt tcttttttat tttactacga aaaacctgtt tatcagctgt ttgatcagta
     7861 atcttttttt tgctatcact ggactcagct tttttctggt ttttgatctg atggattatt
     7921 taggaaagag acatcttgga acagcaagtt tttttgcact acttatcgca tttattttct
     7981 atctattagt aaaaagtata gacaaacaga cgagatttct caacttgctt tttacttatc
     8041 caactgaaca ctattttgga ggtatttgta ttttattaag ctggggcgtg atttacttgg
     8101 tattgggaag tgttcacttc cgctcatcta attatctgct tctatctctt ttgtcgatag
     8161 tcatcagttt tttttctttg tttttttcca ttatgttgat cagtttccaa gtaaaagaaa
     8221 gacaacatca ggaatcgctt caactgtatc aaataaatga agaatattac catcatctag
     8281 aggaattcaa acatgattat aaaagtctac tgttttcatt aaaaattact cttcaggaaa
     8341 ataaaaaaga aacgttggaa tttttagaaa tattagaaga tcattctgca gctatttttg
     8401 aaaaaccgca aatacaacag ctaacaaata ttgtatcccc agctgtcagg ggtgtctttt
     8461 taaaatttct agaaaaagca gaatcggaga aaatcccgat ccaagtagtt attcctgtcg
     8521 aagtgaaaac aatcccgatt gatctggttg tttttattcg ctgtttatct atttttattt
     8581 ctaacgcatt cgatcatcgt gtagccgatc agagtccgat tacgatcaat ctgctggaag
     8641 aaaaagaggg atcacgtttt agtatagcga acaaggcaaa tccaacgaat ttgacgctat
     8701 cggagatggt caaaagagga aaaacctcta aaaaaaatgg cgggttaggt ctatacagtg
     8761 cgaaaaaaat gttggatgtt tacgaaaatg cagaactaaa aatcgaattt tcaaaacaga
     8821 atcactattt ttttgtagaa atgtatctgg catctagaaa gagcaaatca gcctactgac
     8881 ttactgtcaa aggataagtt tgaggaaagt ctacacggag atagagacaa caacacagca
     8941 tattcaaaag aaacagaagc gtgatgaccg ttgtcaccct tctgtttctt ttgctatgta
     9001 gttaaacaaa gtagttgaca cttccgctac ttagtattag tacgaaagta tacttcatga
     9061 tactaagtag ataagaagtg aaaagcggaa cccacgatcg tcaaaatgct aactgctttg
     9121 cttacaccag ataaagggaa gagcaatatc gcttatttta ggcgtggaga agcaagcaaa
     9181 tccctacgat cacaaaaaaa atgctgaccc agcagttaag agagttagaa caagatgaaa
     9241 gtatccatcg aaaagcctat aaccaagtcc cgcctaaagt agagtactcg ctcaccgaag
     9301 aaggaaaatc attaagagag attctagtag cgttgtctgt ttgggaagaa gaacgtatcg
     9361 aaaaacaaaa agcctcaagg gaagaagaca cgctactgca tacacatggt ggctacctaa
     9421 attattgatt ttctatgaca tttattttaa ataaaagaaa acaggagttt ttttaaaagc
     9481 atcatctatt ttttatgtaa aaaaatggta tgattaatca agtaagaagt gattggaaga
     9541 aggcaagcaa atgaatagta tggaaaaagt caaccagttt agagatgaac gtaattggcg
     9601 acaatttcat aacgaaaaag atttggctat ctctatttct ttggaagcat ctgaattgtt
     9661 agaacttttt caatggaaaa cgccggaaga agtaaaagaa acacaattag aacgaatcaa
     9721 agaagaactg gcagatgtat tgatctattc atacatgatg gcagacaacc ttcacctgga
     9781 tctagatgag atcattgaag aaaaattgat caaaaataat ttgaaatatc ctgtagaaaa
     9841 aagcgcaggt gtacgaaaaa aatacacaga gttgtagaaa tgtgtcattc ggctgttgga
     9901 aagagcaaaa gaaaaacagt aaaatgagtc tggagggaga gaaaatgaaa aaaaaaggaa
     9961 aagcaaaaag atggctagtc aatatcctct tgttcctctt gttattagta ggactagctt
    10021 tgattttcaa tgaacagatc aaggattatt ttgtcagaga gacaggagat aaatatgcta
    10081 tagcgaatgt aacaaaagaa gatctgaaaa agaataacga taaagatgtc agttttgact
    10141 ttgatgcggt agaaccaatg acgacagaag gggtcatgag gtcgcaaatg agaggaacag
    10201 atctaccagt gattgctagt atcgcagtcc cttcagtttc aatcaattta ccggttttca
    10261 aaggcttgga taatacgtca ctactttacg gagcaggaac attatctcca gatcaagaaa
    10321 tgggaaaagg aaattatgcc ttagcaagcc atcgtgcaac gaaccctgaa ttgctgttta
    10381 caccgctaga aaatttagag atgggtgcca agatctattt gacagattta gaaaatgtgt
    10441 atacatacaa aacatttttt aaagaaaaag ttgctcctac agatacccaa ttactgaatg
    10501 aagtcgaggg aaaagaaatt gtcacactaa ttacttgtgg cgacatggac gccgtcacgc
    10561 gactagttgt acaaggagaa ctggaaagtg ttacatcgat aaaagatgcg acagatgata
    10621 tgagatctgc tttcaatcta gagacaaaaa cattttagga tactgggaca taagtcgtta
    10681 cagattaaaa acagccaaag gatatgaatc gataaggaaa aaccaatttg ctgaaaaaaa
    10741 gagaaacgca aagaggtgca tctctgattt ggttgttttc tagatagact tgtgttccag
    10801 tttttttatt ttttatatga aaaggcttgc ataaataaaa attcgatggt atgatgattt
    10861 tgaataagaa atggattgtt actgtacggc aggcaaaacc agaattcagc taagaaacag
    10921 taggcgaatt ctaggttttt ttatttttct tgagagggcg aagcaaaatg aaaattgata
    10981 aaattttgaa taacaatgtc gtcatttcga aaaatggatt cggggaagaa gtcgtttgta
    11041 tgggaaaagg actagctttt caaaaaaaga ttggggacga gatttcgcca gaagcggtcc
    11101 aaaaagaatt tgttttaaga gattcttttg ctaaaaacca attccagcag ctgatggctg
    11161 atattccggc agaggagatc gaatgggtca aacaagtagt cgaactggct gaagataaac
    11221 taaaagttga attttcgcct aatatttatc tgacattgac cgatcatctt cattatgcga
    11281 tcacacgagc aaaagaaaat atccaactgc ctaatccatt attatttgaa acaaaaaaat
    11341 tttatccgaa agaatatcaa gcagcgaaag aggctctttt gttcgtgaaa caaaagacgg
    11401 gtgtggaact ttctgaggat gaagccggtt ttattgcctt tcattttgtg aatagccaac
    11461 aaggaaacga agacatgcaa gtgacgatga ccgctaccac aatggtaaga gatatactga
    11521 gtatcatcag taaattcttc ggcatcgtgt tcgatgaaga atctttgaat tatcagcgta
    11581 tcattactca tctgcagttt tttgcccagc gttatctcaa aggggaagga tctgacgagc
    11641 aagatgaatt tctctacgca ttagttcaag gaaagtatcc aaaagcgttt cattgtgtcg
    11701 aacggatcaa tgagtatctt cgacaaacgc aaaaacagga aatggggatc gcagagcaga
    11761 tttatttaac gattcatatt caaagagtag taagtgagaa aaaaagcatc caatcataag
    11821 gattgttact gtatggcagg caaaacctgg attgaaaaaa taggttcacg aatgtttgtg
    11881 actgtttttt tcaatctggg tttttatttt ttaaataaaa aagaaagaag gaaaaaacaa
    11941 tggataatca agcagttggt cgtcgtgtat gggaagcggt aggaggacaa aagaatgtca
    12001 aaagtttagt acattgtgct acgcggttgc gattcagatt aaaagatgaa tctttagcag
    12061 atacgcaaaa gttaaaagaa gacccggacg tgatccaagt cgtacaaagt ggcggtcagt
    12121 atcaagtagt gattggcagt aatgtagcag atgtttatca atcgatcgtg gatgaggaag
    12181 gtcttgcagt ttcggaggat ggaaaagaag agtccaaaaa tattttgaat cgtctgatcg
    12241 atattatctc tagtatattt acaccttttc taggggcgat ggcagccgcc ggggtattga
    12301 aaggattctt atcattggca acggtgatgg gctggctgac agctgatagc ggtacttatc
    12361 aaatattgtt tgctgcagca gatggtgtct ttaccttctt acctgtcatg cttgctttta
    12421 cagcagctaa aaagttcaaa gccaaccagt ttttagcagt agcaattgct atggcacttg
    12481 tgtatccagc tatcacagca gttgctggtg cgggagaagc tatcagtttc tttgggatcc
    12541 cagtgattct ttcaccatct ggttatactt cttctgtcat accaatcatt ctagccgtat
    12601 gggttcaaag taaattagag ccattcgtaa aaaaagtgat tccgcaattt ctgcaaatga
    12661 ttttagttcc gcttgtcgtt ttagtagtga tggttccatt aacatttctt gctttaggac
    12721 caatcgggac ggttgccggt aatgctttag gcggtctctt caactcgatt tatggattta
    12781 gtccaatcgt agcaggtcta atcatgggaa gtttatggca agtattcgtg atgttcggca
    12841 tgcattgggg attcgtaccg atcatgtttt tgaatatcga acaatatggg tttgatgtat
    12901 tgatgccaat gcttcttcca gccatcttgg cacaaggtgg ggctgcttta gctgtagcat
    12961 tgcgaacaaa agatacgaaa cttcgagcat tagggatttc ttctacagtg acttcgttat
    13021 ttggaatcac tgaaccaact gtgtatggtg tgacattacc tttgaagaaa ccattcattg
    13081 ccgcttgtat ttctggaggt attggtggag ctattatcgg attttccgga gtaaaagctt
    13141 tctccagcag tttggtcagc ttattgacga ttccgacttt cattaatacg gtggatggtg
    13201 tagagtcaaa tgtgacagta gcagtaatag ctacaggaat tgcttttgtt cttgcattcg
    13261 taggtacatt gattttagga tttgacgaac aaacacaaga caatcaatta gaaaacaaac
    13321 atgcgaatgc aggaaaaccg attacttcag cacggcatac cttaaaaagt cctttaacgg
    13381 gtaaagtact gccattgagt gaagtaccag accaagtttt ttcttctggc gtgatgggaa
    13441 aaggtatcgc tattgatcca gaagtaggag aactagttgc acctgcagac ggagagatca
    13501 cgactatttt tccaactgga catgctgtag ggatcacaac gactgacgga gcggagattt
    13561 tgattcatat cgggatggat acagtagaat taaatgggaa tggctttgag atcttagtca
    13621 aacaagggga tctcgtcaaa gcaggagatt tattgattcg ttttgacatc gaagcaatta
    13681 gagcagctgg ctatagcgta atcactccag ttgtcattac gaatactgat gcatttgccg
    13741 atatacttga attagaccaa aaagagatta ttgcaaacga agatgttctg gcaattgtaa
    13801 aataaggagg aaatcaggat gcaatcacgt ttttcagaaa aatttttatg gggtggagca
    13861 actgctgcca aatcaattag aaggggctta tgatcgagac ggaaaaggac tatccgttgc
    13921 agatgctatg cctggaggta agcagcgctt tgcgattatc ggcagtgaag aatttgactg
    13981 gacgattgat caagaaaaat atatttaccc gaatcatcga ggaatcgatc attacgatcg
    14041 tttcaaagaa gatcttgcct tgtttgcgga aatgggattc aaatgttatc gtttttcgat
    14101 tgcctggaca cgaatttttc caaatggaga cgaaggtact cctaacgaag cgggattaga
    14161 attttatgat cagctgattg atgagtgctt aaaatatgat attgaacctg tgatcacgat
    14221 ttctcattac gaaatgcctc ttcatttggc aaaagaatac ggtggctgga aaaatcgaaa
    14281 actgatcgac ttctatgaac gatttgctca gacagtatta gagagataca gcagtaaagt
    14341 caaatattgg atgactttca atgaaatcaa ttcggccttt cactttccag ctttaagtca
    14401 aggattagta aaaagcaatg gagcaggaga gtaccaaaat attttccaag catggcataa
    14461 tcaattcgtt gcaagtagca aagccgtaaa aatcggccac gaattgcgtt cggatatcca
    14521 aatcggctgc atgattatct atgcaacaac ttacagcatc gatgccaatc cagtcaatca
    14581 ggcagcaaca atgatccaaa accaagaatt caattttttc tgcacggatg tccaagtacg
    14641 tggagaatac cctgcataca cagcgcgtac ccataaaaaa tatggagtcg atccggaaaa
    14701 attagagcaa acggaaaaag acttccagtt gctaaaagaa tatccagtgg actacatcgg
    14761 atttagttat tatatgtcta cggcaatcaa tgagactgat ccacaagcag caacttcaga
    14821 agggaacttg ctgggaggag taaaaaatcc gtttcttgag gcaagtgaat ggggctggca
    14881 gatcgatcca gaagggctga gaatcgcatt gaatgaatta tacaatcgtt accagaaacc
    14941 attattcatt gttgaaaacg gtcttggagc cattgatcat atggaagagg atggcacgat
    15001 tattgatgat tatcggatcg attatttacg tcgtcatatc gaagcgatgg ctaatgcagt
    15061 agcagacggt gtagatttga tgggctatac gccttgggga tgtatcgatc ttgtcagtgc
    15121 atcaacagga gaaatgagta aacgttacgg ctttatctat gtggatctag acgatgaagg
    15181 aaatgggaca ctgaatcgat caaagaaaaa atcttttgat tggtataaag aagtaattgc
    15241 ctcaaatggc gtaaacttgt aattaaagca gctgaatgaa aaagaacaca cttctcacat
    15301 gtgatttgtg ttcttttttt attttttatg tgctattctt ttagtaatag taaagcaaag
    15361 ggtgacgtta tgaaaaggac acgtgtacta tacttggaag tcgcagataa aattaaagaa
    15421 gatattttct caggaaaata tccagtcggt tcgatgttgc cgacagaaag tgaattagaa
    15481 gaactgtttc aagtcagcaa aatcactgtc cgaaaggcaa tcgaactgct ggcatcagat
    15541 gaatatgtcg caaagaaaag tggtcgtggg acgacggttc taagcaatcg tccttacaat
    15601 cgattatcca aagcggcaag ctttacgcaa atcttggaga actcccgata tgaagtccgg
    15661 aaagaaacgc tggatttgca attggtccat ataaagaagg aagatccttt gttttccttt
    15721 ttcggtgagc aagctgtttg ttttaagcgc ttatattatt tgaacaatca gccttatatt
    15781 tattttgttc attatcttcc acaagaaatg atgcaattca gcagagaaga atttggcaaa
    15841 gaatctttgt atcgtttgct gacgaaaaaa ggatatacta ttgactcatt ttctgatgat
    15901 ttctcggcag tatttttaac agaagaagaa caaggaattc tgaaaacaga cgcacagatc
    15961 ggcatcaaac ggatacggaa atcgtctacg caaattgggg aaatgattga atattcggag
    16021 gcggtctacc atacggagct gcatccttac catattgatt atgaaacata aataaaaaaa
    16081 gaggttgtga aaaagctgcc ctgcatcaag taataagaac agccaaacaa aaatagcttc
    16141 tcatattttt cgtttggtta ggcttattac cgcagataca cggctgtttc tggattacca
    16201 tttattctgg ataagggggt gtggcacgat tttcagaagg aatctatgaa ccaatcactg
    16261 tattgatttt agcttcgatg gtaaccccat tcggcttgac gatcgcttat ttcttaggga
    16321 aaatcattcg taaaaatata ctcaatcgtc aagaaatcga tacattgaag accgcttttc
    16381 ctatggggat ctgtcagatc acagagggat gtttccctat cgtattgaac gacttggcac
    16441 gcaatgtcat agcaacggga gtcggtggga cagtaggtgg cggattaagc atgttttggg
    16501 gcgcagatag ccgtattccg gcatcgggaa tgtttgcagt cgctacgatg accagaccat
    16561 gggcattcat tggtgcattg ttagctggtt cttttgtcac aggaataacc attttattat
    16621 tgaaaaaacc agtagatccg aatgcagaaa tcatccaaga agaaaaagag gaagaagaca
    16681 tatcttggga tgatttgaca attagttagg agaggcagaa tggattacaa gaaaaaagta
    16741 caatagatgt accttgagac aggaatggta tgaaagaatc agagttagaa agaatcgaat
    16801 atacagattt tggtttagga gcgatcgaaa ctgtccagag catcggcacc ctccttgaaa
    16861 tgacgatgaa ggaaaaatga gaacctttcg ttgtcgaaag ggtctggtat accttatgtc
    16921 gaaggaaagg aaacaaaaaa actttcctgt actattccaa aaaggaaaga aagttattat
    16981 acagtaaaaa aagaaattat tcttcatgct agagatcagt acacgttcga gcccaatatc
    17041 aaacactagt tccaggcagg cgcaacaggt gcggttatca ctgaattttc ttccagcagt
    17101 gacgattcaa gtgatatttt tacaaatcca gctatttatc gttaacaaaa agatttttag
    17161 gagaagctga tgaacaaaaa tgatcaaatt ttgatcgttt agttcgtcgg cttttttatt
    17221 tagagaaaca tcatttatca gtgaaacata acacgagaac aaaatgattt tttcgttaat
    17281 aaaagaaaca agtatttgtt gtttaaaata atacacgtta ttggca
//
LOCUS       DACDKM010000052        17156 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20834_2016
            SAMN12640370-rid9891663.denovo.052, whole genome shotgun sequence.
ACCESSION   DACDKM010000052 DACDKM010000000
VERSION     DACDKM010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640370
            Sequence Read Archive: SRR10040891
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17156)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17156)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742835.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742835.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:04:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,012
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 29 of 75
            Pseudo Genes (incomplete)         :: 33 of 75
            Pseudo Genes (internal stop)      :: 19 of 75
            Pseudo Genes (multiple problems)  :: 5 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17156
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640370-rid9891663.denovo.052"
                     /isolate="E20834_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            144..1910
                     /locus_tag="IV334_001610"
     CDS             144..1910
                     /locus_tag="IV334_001610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008271692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glycosidase"
                     /protein_id="HAP8943681.1"
                     /translation="MDTAAIYHRPESEYAFLYTNTRFRIRLRTRKNDIKKVYVLCGDP
                     YTITTEKWYQKQRPMKKCLSTNVHDYWEIEVTSETSRLQYAFHVVGEDNTDCFYGDQG
                     IFPYRENVITEPNFYFRIPYFHQIDRFTIPEWVKETVWYQIFPERFANGDKANDPEHT
                     LPWGSKDPDREDFFGGDLQGVMDHLDYLTDLGINGIYFCPIFKATSNHKYDTIDYYEV
                     DPAFGDKKLLKNLIDEAHKRGIRIMFDAVFNHMGVHSPQWQDVLKNGEKSIYKDWFHI
                     RFFPVDSYQMTDLPETAENIPYDTFAFTPFMPKLNTANPEVQKYLLDIATYWIKEFDI
                     DGWRLDVANEVDHHFWKKFREAVTEIKPDIYILGEIWHSSQAWLQGDEFHAVMNYAFT
                     DSIKDYFAKKKITASQMVSGMNHQQMLYRDQVNEGTFNLLDSHDTARILTLCQGNKEL
                     MKSVMAFMFLQKGSPCIYYGTEIGMTGEDDPDCRKCMIWEKEEQDLELFGFIKELVSL
                     RKQLSKIISEGSTQWLIVNDREDKLYFTRKLEGQIIYVYFNQSKEPWVVEQENEVILS
                     QNCQLLEDGKAEIRQYGFLIMC"
     gene            2012..3271
                     /locus_tag="IV334_001611"
     CDS             2012..3271
                     /locus_tag="IV334_001611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HAP8943682.1"
                     /translation="MKKRSVKQLGLGLISLGILGGLAACGNGNSSAEGEADEGKTLTV
                     SVDSGYKDYINEIKNTFEKENDVKIKLVEKDMFDQLESLALDGPAGKGPDVMMAAYDR
                     IGALGQQGHLAEVKLGNEAAYDETDKAQVTYDGKIYGEPAVIETLVLYYNKDLVDTAP
                     STFKDLENLSKDSRFAFESEAGKNTGFLAKWTDFYYSYGLIAGYGGYVFGDDGTNPSD
                     IGLNNAGAVEGISYATDWFKNVWPKGMQDIKSAGDFASQQFMSNKTAAIIDGPWQAQT
                     YKENNINYGVAKIPTLNNGQPYQPFGGGKGWVVSNYAKNKDLSQKWLDYVTNQENQEK
                     FFEMVNEIPANQQARETAKAKNDELTTAVIEQYETAQAMPNIPEMGEVWTGAENLMFD
                     AASGSKTPKESADEAVKTISEAIEQKY"
     gene            3418..4719
                     /locus_tag="IV334_001612"
     CDS             3418..4719
                     /locus_tag="IV334_001612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP8943683.1"
                     /translation="MQQDKTSNINEKKITLCSLIPGMGQFVNGQAFKGIVFFAIFVLF
                     IGQMLLGGFHALVGLVTLGSIPMEDHSLFILIEGTLQFIITIIFLIFYGLNLLDAYRV
                     AKMRQEGKKINRTMKEVIHNIGDEGFPYLLTLPAYLLMIFTIIFPVLVTLFTAFTNYD
                     FNHIPPASLIDWVGAKNFFSIFFLSSYRNTFFAVFSWTVIWTLCATTLQITLGILTAV
                     VAHQKFIKFKRIFGIIFLLPWAVPAFITIMSFSNIFNDSVGAINTQVIPFINQLPFVD
                     ISAISWKTDPNWTKVAIILVQGWLGFPYIYVMVTGILQSISEDLYEAAKIDGANAWQR
                     FSNITLPAVFLVAAPTFITQYTGNFNNFSMIYLFNNGGPGSVGGGAGSTDILISWIYK
                     LTTGTSPQYSMASAITLIISVIVICVSLLVFKKTNAFNMGD"
     gene            4722..5573
                     /locus_tag="IV334_001613"
     CDS             4722..5573
                     /locus_tag="IV334_001613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP8943684.1"
                     /translation="MKKFHSSARFQKRLSHFFTYFFLLILSVIIIYPLLITASTAFKS
                     GNIAAFSLDLSSDWTLGNFTRLFSETLYGTWYKNTLIISVLTMIVQVTVVTLAGYTYS
                     RYRFIGRKSSLMFFLVIQMVPTMAALTAFYVMAFLLEALDKYWFLTMIYIGGGIPMNT
                     WLMKGYFDTVPIDLDESAKLDGAGHFRIFFQIVLPLVKPMLAVQALWAFMGPFGDFML
                     AKFLLRTPENQTVAVGLQTFISDVTNQRVSLFAAGAILIALPICLLFFFLQKNFVTGL
                     TSGGTKG"
     gene            5586..6401
                     /locus_tag="IV334_001614"
     CDS             5586..6401
                     /locus_tag="IV334_001614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrose utilization protein MalA"
                     /protein_id="HAP8943685.1"
                     /translation="MQTFPLNYFSALRTPTQVFAGRKLLSWPKFFLIFVFLVSLMVMP
                     VTLFYANQIQAIPLEQFLSVHSLIDEQGTQKFSELELSETGLQASQQTIAVTPEILVG
                     VSLSEKQQSDHGTFIDFEKEQWVIQQKDKSGIRRYTMNYSPSFQPDSVRTPEDFQRFL
                     EREFYASNRPTIILSYSLSLGLVLFVMTSLILFGASFFLWMTRKSQLSSIHTFKESAN
                     LMLNVMGIGSMAAAVVGFIHFDFILMLSVQTTITVLLLLWVFVKTRFKDKRVE"
     gene            6552..7718
                     /locus_tag="IV334_001615"
     CDS             6552..7718
                     /locus_tag="IV334_001615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAP8943686.1"
                     /translation="MLEKTLYNQLFSRSFSLPVEVNYWDGTTKQYGDTDTPPQIKINI
                     HEEIPMKEITSNASLALGEAYMDHRIEIEGSIQALINDAYQQANSFLRGKDYLKWLPK
                     KEKHTKQKNKEDIHAHYDLGNDFYKLWLDPTLTYSCAYFNTPEDTLEQAQINKVHHIL
                     DKLFIKEGETLLDIGCGWGTLMFTATKEYNVKATGITLSEEQYDLITKRIKEEHLEDK
                     CRVLLMDYRELKGETFDHITSVGMFEHVGSENLEGYFKVVKDLLKPKGTALIHGISRQ
                     QGGATNAWINKYIFPGGYVPGVTELVDNITKNDLQLIDLESLRRDYQLTLEHWTKNFH
                     QVQDQVTKEMGDSFYRMWDLYLQACAASFESSNIDVIQYLLVQPGNNDIPMHRA"
     gene            7948..8595
                     /locus_tag="IV334_001616"
     CDS             7948..8595
                     /locus_tag="IV334_001616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysis protein"
                     /protein_id="HAP8943687.1"
                     /translation="MKKTVIYTSLFAALFLAACQSNTNQGTSGNTSSSSEQRSSQVSD
                     SSTKASTSSSSQTKSSSEASTQSDIKVSAARAIELFQDKYPDTAITSLELDSDWGSYF
                     YKIEGVDDQKEYEARIDAQNEKMETENPETLDRDEQNGQKKKEDGLDVDKLITIEEAA
                     KIAVDKVGSGTATDWDLDKDLGTTYWEVKVKDGNQSTSVKINSQTGEVLSTEQDD"
     gene            8679..9623
                     /locus_tag="IV334_001617"
     CDS             8679..9623
                     /locus_tag="IV334_001617"
                     /EC_number="1.3.98.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate oxidase"
                     /protein_id="HAP8943688.1"
                     /translation="MSLETTFANHIFANPLMNASGVHCMTTQELDELAHSEAGAFITK
                     SCTINERKGNPEPRYFDVPLGSINSMGLPNLGFSYYLEYALAYEKVQENQNQPLFFSI
                     AGMSVQENLEMLEKIEKSGFNGITELNLSCPNVPGKPQLAYDFEATYETLKEVFSIFS
                     KPLGIKLPPYFDFAHFDQMADILNQFPLTYVNAINSVGNGLYIDTEQEAVVIKPKEGF
                     GGIGGEYIKPTALANVRAFYTRLKPEIQIIGTGGIRTGQDAFEHLLCGASMLQIGTEL
                     HKEGPEIFSRIIKELTQIMSEKGYTSIDEFKGKLRTIS"
     gene            9758..11209
                     /locus_tag="IV334_001618"
     CDS             9758..11209
                     /locus_tag="IV334_001618"
                     /EC_number="3.5.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HAP8943689.1"
                     /translation="MNDATELAKELKEKEFSFQELVTDIQKKIKKQNHELNAFVALEP
                     FESNSHELLIPQSEKGPLAGIPFPLKMLGQEKKGWLATSGSRLFETHRASHTSNYVRQ
                     AEAIGLVPFGQTNAPEFGFKNITDPVIYGPARNPWNLECTPGGSSGGAAAAVSSGIVP
                     LAGASDGGGSIRIPASFCGLIGLKPSRGTMPVGPYAWRGWQGAAIDFGLTVSMRDTEA
                     LFYGMRSIHSGAPYQVSPVEWQTHPRKKRLKIAFCVDSPIHSQISEEAVLAVKKAVDF
                     LGKEGHDLTEIPYPLDGRKLITSYYQMNGAETAAMIRSIETGIQRAVRKEELEPISWT
                     LFQYGQKLSAATYIDSLHVWDEAAIVMENLHQEYDLFLSPTTAFTAPKISEDLQSDAI
                     RAQMEHISDCSEVEGLDVVSSMFEESLKITPYTQLANLTGQPAISLPTHLTKENMPLG
                     IQFMAARGREDLLFQIGYLFEANHQFHLPNPWK"
     gene            11230..12066
                     /locus_tag="IV334_001619"
     CDS             11230..12066
                     /locus_tag="IV334_001619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HAP8943690.1"
                     /translation="MKKKRLFALITSAFFILSACGTAAANQEDNSWSNVEKKKELVVA
                     TSGTLFPASYYNDQNELVGYDVDLAKEVAKRLNVKISFKEYNVDGQITSVSKRESDFA
                     ANDFGITKEREKKFSLSETFKYSFDSMIVRKKDDSGIHSLEDLKGKKAAGEPNTSYMR
                     LAEKHGAKLVTYDNATNDQYLSDVANGRTDVILNDYYLQKMAVSALPDIPVKILENVY
                     FNENESGFLLKEGNDALKKKIDGVLKEMKEDGTTKKISEKYFQTDVSVKPKETMAESV
                     SE"
     gene            12066..12725
                     /locus_tag="IV334_001620"
     CDS             12066..12725
                     /locus_tag="IV334_001620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HAP8943691.1"
                     /translation="MYFPLIDFSLMWTSLPFVLQGLVYTLGIGFVSFLLGNLVGLLLT
                     VLGLLDWLPLNVFIRFYLSFFRGIPALVLLFLLYFGLPYQLSALTASVICFTITSSAF
                     IGEIYRGSLAGVSSGQWDAGLASGFSFFGVLKYIVLPQAFRISVPALSNVAMDLVKGT
                     SLAATITVPEIFQKAKIIGGRTFDYFSLYILVALIYWVICIGIEKVQQYLEKRSMHQS
                     I"
     gene            12851..13540
                     /locus_tag="IV334_001621"
     CDS             12851..13540
                     /locus_tag="IV334_001621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8943692.1"
                     /translation="MKTPLTIEIESSLYQYCIEQGGLVVEEVTMPDDQGIVDTLSCLT
                     KFDGSREWRCYELKVTKADFRSTAKLSFVGHYNYFVLPSKLYEEVKTEIPTGIGVLVY
                     RPYTVEEEMPASGTFLIAKKAAKKELAVPEEALTNRFMASLFREVRKAKQMERGVKYF
                     PTDQLYKELKRRSENQDPFSNERYYERFLEEIASQAINELQEEVIALQHDYEYLRQQQ
                     QIRRLPTEPLE"
     gene            13546..14502
                     /locus_tag="IV334_001622"
     CDS             13546..14502
                     /locus_tag="IV334_001622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sce7725 family protein"
                     /protein_id="HAP8943693.1"
                     /translation="MYYPYLRGKQFDLLALKEALSRGLLSNKIQPVIEPVRDSATLKN
                     VIELFQKKHHPIAVIQNPQVGQFKLFDQHVHSWTVKENSSVVPAQILTPENLSEVLDS
                     PPAFLVFDGQHYPKDTEVWKQLAGVDSKFLIPDTSRFRIWLPENKIVIRDSFQTRKHV
                     ESYADKTDDFFSDDYLFFHADGYIGFSDFTIEGSRYFDKGFPSRALAIHLTYIDAYGN
                     IRVKHFVSDSNDSAKDQALKFLEAGDKMKKWIMRHHHQLLITSGMIELLALYQQQKFP
                     GLGVLKKWSLMHHLELISHLLDHPKDWLKYYSKVQELYEVIQ"
     gene            14546..15058
                     /locus_tag="IV334_001623"
     CDS             14546..15058
                     /locus_tag="IV334_001623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP8943694.1"
                     /translation="MYIRQTRKEEVEEVVEIINAAADFIASQGSPQWQNGQKPTKETI
                     LNDIERKESYVLIVEDQIAGTAALVSSVDPVYTKIDGSWKESTGPYLSIHRVALAEST
                     RGKKLAKPFLEHLLTIAASKNIQDIRIDTYPANEPMEKTIYSAGFTYQGMIEFPFEHG
                     ERKAYQIIIN"
     gene            complement(15314..15862)
                     /locus_tag="IV334_001624"
     CDS             complement(15314..15862)
                     /locus_tag="IV334_001624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAP8943695.1"
                     /translation="MNYCYVFIGPSGSGKTSLAEAIFQPEQKIITYTNRSPRPGEKDQ
                     EDYYFVSDNTFNQMIKGNEFAEWDQYAQHKYGSSKAEIKKKLTVGNCYAVLTANGFWH
                     LYEYFGTVVRPIFITISKETLQSRLKKRGDSSEQISARLAVYVEDLAQLKRLKAIPSL
                     LVVSNDGAFQEAADKIKKGLLI"
     gene            complement(15951..17132)
                     /locus_tag="IV334_001625"
     CDS             complement(15951..17132)
                     /locus_tag="IV334_001625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HAP8943696.1"
                     /translation="MSYLLGIIGLFVTLGLAFLISSNKKAIKLKPLIVMFVLQLVLGF
                     VLLRTTFGTAVVSVLAKMFDHLLAFAGQGVDFVFAGVANKGSAPFFLSVLMPIVFISA
                     IIGILRYIKILPLFMKAVGLGLSKINGMGKLESYNGVASAILGQSEVFISIKKELPFL
                     SEKRLFTMSVSAMSTVSMSIVGSYMALIDSKYVITALVLNLFGGYILASIVNPYVLEE
                     KEDELIIEENKEQTFFQMLGEYILDGFHVAITVAAMLIGFVALIAMINAIFHGIFGIT
                     FQEILGYIFAPLAFISGIPWKEAVDAGSIMATKLVTNEFVAMTELANGNFHFTERTTA
                     IISVFLVSFANFSSIGIISGAMKGLNEEKGNLVAKHGLKILLTATLVSFLSAIVTGLL
                     V"
BASE COUNT         5633 a         3065 c         3444 g         5014 t
ORIGIN      
        1 tttaagtcta tctatttggc aagtttaaaa aataaaaatt cggtatcaaa aaactgttac
       61 cgataacatt tttgaaagcg ttgtcttttg cttctctatc acttatagtg atgacgtcat
      121 attaaaacac aaggaggaaa tcaatggata cagctgcaat ttatcatcga ccggaaagtg
      181 aatatgcatt tctatatacg aatacaagat ttcgtattcg gcttcgtact aggaaaaatg
      241 atatcaaaaa agtatatgtg ctatgtggag atccatatac cataacaact gaaaaatggt
      301 atcaaaaaca acgaccaatg aaaaaatgtc taagtaccaa tgttcatgat tattgggaaa
      361 ttgaagtgac gagcgaaaca agtcgcttac agtatgcgtt ccatgttgtt ggggaagata
      421 acacggactg tttttacggt gatcaaggaa tttttcctta tcgagaaaat gtgattactg
      481 aaccaaattt ttattttcga ataccttatt ttcatcaaat cgatcgattc actattccag
      541 aatgggtaaa agaaacagta tggtatcaaa tttttcccga aagatttgca aacggggaca
      601 aagcaaatga ccccgaacac actttacctt ggggaagtaa agatccagac cgagaagact
      661 ttttcggggg agatttgcaa ggtgtgatgg atcatttgga ttacttgacg gaccttggca
      721 tcaatggcat ctatttttgt cccatattta aagccacatc caatcataaa tacgatacga
      781 ttgattatta tgaggtggat cccgcttttg gcgataaaaa acttttaaaa aacttgattg
      841 atgaagcaca taaaagaggg atacgtatca tgttcgatgc tgtattcaat catatgggtg
      901 tccattcacc acagtggcaa gacgttctca aaaacggaga aaaatcgatc tataaagact
      961 ggttccacat ccgttttttc ccagtagatt cttatcagat gactgactta ccagaaactg
     1021 ctgaaaatat cccatatgat acttttgctt ttactccatt catgccgaaa ctaaatacag
     1081 caaatccaga agtgcaaaaa tatttgctgg atatcgcaac ttattggata aaagaattcg
     1141 atattgatgg atggcgtctt gatgttgcta atgaagtcga tcatcatttt tggaaaaaat
     1201 tcagagaagc agttacagaa atcaaaccag acatctatat ccttggagag atttggcatt
     1261 cctctcaagc ttggctgcaa ggggatgaat tccatgcagt gatgaattat gcatttacag
     1321 attcgattaa agattatttt gcaaaaaaga aaatcacagc ttcacaaatg gtcagtggaa
     1381 tgaatcatca gcaaatgctt tacagagatc aggtgaacga aggaacattc aacttattag
     1441 attctcatga tactgccagg atcttgaccc tttgccaagg taacaaagaa ttgatgaagt
     1501 ctgtgatggc ctttatgttt cttcaaaaag ggtctccttg tatctattac gggacagaaa
     1561 tagggatgac tggtgaagat gacccggatt gccgcaaatg tatgatatgg gagaaggagg
     1621 aacaagatct agagctattc ggttttataa aagaacttgt ttctctaagg aaacagttgt
     1681 ctaagattat ttctgaaggt tctactcagt ggctaatagt aaatgatcgt gaagataagc
     1741 tctatttcac acggaaacta gagggacaga tcatctatgt ttactttaat cagtcaaaag
     1801 aaccttgggt agtagaacag gaaaatgagg tgattcttag tcaaaattgt cagttgttag
     1861 aagacgggaa agcagagata cgacaatacg gatttttgat catgtgttga aaactgttac
     1921 cggtaacaac aagaaagcgt ttgcaaatga agatttgtgc ccattataat aagggtgtaa
     1981 cagcacgttg aatacaaagg aggaaatgaa gatgaaaaaa agaagtgtga aacagttggg
     2041 tttagggtta atctctttag gaatcttagg cggattagcg gcttgtggca atggtaactc
     2101 ttcagcagaa ggggaagccg atgaaggaaa aacgttaaca gtttccgttg attctggata
     2161 taaagattat ataaacgaaa taaaaaacac tttcgaaaaa gaaaacgatg tgaaaatcaa
     2221 gctagtagaa aaggacatgt tcgatcagtt ggaatcatta gcgttagatg ggcctgctgg
     2281 aaagggacct gacgttatga tggcagcata tgaccggatc ggtgcgttag gacagcaagg
     2341 acatctggcg gaagtaaaac tgggaaatga agcagcttac gatgaaacag acaaagccca
     2401 agtgacttat gatggaaaaa tctacggaga acctgcagta atcgagaccc ttgttttgta
     2461 ctataacaaa gatttagtcg atacagcacc atcaacgttt aaagatttgg agaatctatc
     2521 caaagacagt cgttttgctt ttgagtcaga agcaggaaaa aatacaggat ttttagctaa
     2581 atggactgac ttttattatt cctatggttt aattgcaggt tacggtggct atgtatttgg
     2641 tgatgatggc acaaatcctt cagatatcgg tttgaataat gcaggtgctg tagaaggaat
     2701 ctcttatgca acagattggt tcaaaaatgt ttggccaaag ggaatgcaag acataaaaag
     2761 tgcaggagac ttcgccagcc agcaattcat gtccaataaa actgcagcaa tcattgacgg
     2821 tccatggcaa gcgcaaacat acaaagaaaa caatatcaat tatggtgtag ccaaaattcc
     2881 tactctaaat aatggacagc cttatcaacc gtttggcgga ggaaaaggat gggtagtcag
     2941 caattatgct aaaaacaaag acctctctca aaaatggctt gattatgtaa cgaatcagga
     3001 aaatcaagaa aaattctttg aaatggtaaa tgaaatccct gcaaaccaac aagctaggga
     3061 aacagcaaag gctaaaaacg atgaattaac aacagctgtg attgaacaat atgaaacagc
     3121 tcaagcgatg ccaaacatcc cagaaatggg cgaagtttgg acaggtgcag aaaacttgat
     3181 gttcgatgca gcatcaggtt caaaaacacc aaaagaatcc gcagatgaag cagtaaaaac
     3241 aatcagtgaa gccatagaac aaaaatactg attaaaatga taaccgtcca tttttataaa
     3301 aaatggaaca ggtgcttcag ataaaagtgg caagagcgag atggaaaatt atcttgctct
     3361 tgttgccatt atcttaaaag cagtttgcgg tttagcaagt ttagaggggg cattcagatg
     3421 cagcaagata aaacaagcaa tatcaacgaa aaaaaaatca cgctttgttc acttattcca
     3481 gggatggggc agtttgtaaa tggccaagct tttaaaggta tcgttttttt cgccatattc
     3541 gtcctattca ttggtcagat gcttctgggc ggatttcatg cattggtagg attggttact
     3601 ttgggaagta taccaatgga agatcattca ttatttattt tgattgaagg cactttgcaa
     3661 ttcatcatta caatcatttt cttgattttt tacggtttga atcttttaga cgcttatcga
     3721 gtagcgaaga tgcgtcaaga gggcaaaaaa atcaatcgga cgatgaagga agtcatacat
     3781 aatataggtg atgaaggttt tccttatcta ttgacacttc ccgcttatct tctcatgatt
     3841 tttacgatca ttttcccagt cctagttaca ttgttcactg catttactaa ttatgacttc
     3901 aatcatattc caccagccag tttaattgat tgggtagggg ctaaaaactt ttttagtatc
     3961 ttctttttaa gctcatatcg taatactttt tttgcagtct tttcgtggac cgtgatttgg
     4021 actttatgtg caacaacttt gcaaatcacc cttggtattc tcacagcggt tgttgctcac
     4081 cagaaattta ttaaattcaa acgtattttc ggtatcattt tcttattacc atgggcggtc
     4141 ccagctttta ttactatcat gagtttttcg aatatattca acgatagcgt aggtgccatc
     4201 aatactcaag tgattccttt cataaaccag cttccttttg tagatatcag tgcaatctct
     4261 tggaaaacgg acccgaactg gacgaaagta gcaattatct tagtgcaggg ctggttagga
     4321 tttccctata tctatgtgat ggttacagga atcttacaat cgatttcaga agacttatat
     4381 gaagcagcaa aaatcgacgg agcaaatgca tggcagcgct tttcaaacat cacgcttcct
     4441 gctgtcttcc tagtggcagc gccaacattt attacacagt atacggggaa tttcaataat
     4501 ttctccatga tctatctgtt taacaatggc ggtcccggaa gcgtaggcgg aggagctggt
     4561 tctactgata ttcttatttc ctggatctat aaacttacga ctggaacttc tccgcagtat
     4621 tctatggctt cagcaatcac gttgattatc tccgtcatcg ttatctgtgt gtcattgctg
     4681 gtatttaaaa agacaaatgc gttcaatatg ggggattaac aatgaaaaaa tttcattcga
     4741 gtgctcgatt ccaaaaacga ctttctcact tttttactta cttttttctt ttaatactgt
     4801 cagtcattat catctatccg cttttgatca cagcaagtac tgccttcaag tcaggaaata
     4861 ttgcagcttt tagtttagat ctttcaagtg actggacttt aggaaacttt actcggctat
     4921 tcagtgaaac actttacggg acttggtata aaaatacatt gatcatttct gtcctaacaa
     4981 tgatcgtaca agtgactgta gtaactttgg cgggctatac ttatagtcgc tatcgtttta
     5041 tcggccgcaa atcaagtttg atgttctttt tagtgattca gatggtaccg actatggcag
     5101 cattgactgc cttttatgtc atggctttct tattagaagc gctcgataag tactggttct
     5161 tgacaatgat ctatataggc ggtggaattc caatgaatac ttggctgatg aaaggatact
     5221 ttgacactgt accaatagat ttagatgaat ctgctaaact tgatggggca gggcatttca
     5281 ggattttttt ccagattgta ttaccgcttg tcaaaccgat gctcgccgtt caagcattat
     5341 gggcttttat gggacctttt ggggatttca tgttagctaa gttcctgctt cgaacacctg
     5401 aaaatcagac tgttgctgtc ggcttacaaa catttatttc tgatgtgaca aaccaaagag
     5461 tctctctttt tgctgctggg gcaattttga ttgcactacc gatttgtcta ttattcttct
     5521 ttttacaaaa gaactttgtt accggcctga catctggcgg aacaaaagga taggagcaga
     5581 gtatcatgca aacatttccg ttaaactatt tttccgcatt acgtacgcca actcaagtgt
     5641 ttgcgggacg caaactatta tcatggccta aatttttctt gatatttgta tttttagttt
     5701 ccttgatggt catgcctgtg actctttttt atgccaatca gatccaagcg attccattag
     5761 aacaatttct atccgttcat tcgctgatcg atgaacaagg aacccaaaaa ttttctgaac
     5821 ttgaattatc tgaaactggg ttacaagcat cgcaacaaac gatagctgtg acccctgaaa
     5881 ttttggtagg tgtgtcttta tctgagaaac aacaatccga tcatggaact tttattgatt
     5941 ttgagaagga acaatgggtg atccaacaaa aggataaatc tggtatacgc cgctatacga
     6001 tgaattatag cccatctttc caaccagatt ctgtacgtac gcccgaagat ttccaacgat
     6061 ttttagaaag agaattttat gccagtaatc gtccaacaat cattttatct tatagtttaa
     6121 gtcttggtct tgtcctattt gtcatgacga gtctcatttt attcggggct tcctttttct
     6181 tgtggatgac aagaaaaagc caactttcat ccattcatac attcaaagag agtgcaaatt
     6241 tgatgctgaa tgttatggga ataggttcca tggcagcagc agttgtaggg tttatccact
     6301 ttgactttat cttgatgcta agtgtccaaa caacgataac tgtcttgctg cttttatggg
     6361 tttttgtcaa aacaagattc aaagataagc gtgtcgaatg acaagtttaa gcgatttatg
     6421 cgacaatgaa tgtgaaaaaa atgaaaaata aagtagtttt tgaaaaattt ctgaaaaaat
     6481 taaacttctt agttgattct tttctttttt agtattaacg taatagggta gtttttttaa
     6541 ggaggaaaat catgttagag aaaactttat acaatcaatt attttcacgt tctttctcgc
     6601 tccctgtcga agtaaattat tgggacggta caacgaaaca atacggagat acggacactc
     6661 ctccccaaat caaaatcaat atccatgaag aaatcccaat gaaagagatt acaagtaacg
     6721 cttctttggc actaggagaa gcctatatgg atcatcgaat cgagatcgag ggaagtatcc
     6781 aagcattgat caacgatgct tatcagcaag caaacagctt tttacgagga aaagattatc
     6841 taaaatggct gccaaagaaa gaaaaacata cgaaacaaaa aaacaaagaa gatatccatg
     6901 cgcattatga cttaggtaac gacttttata aactttggct agatccaact ttgacttatt
     6961 cttgtgctta tttcaataca cctgaagata cgttggaaca agcacaaatc aataaagtcc
     7021 atcatatctt agacaaactc tttatcaaag aaggcgaaac gttgcttgat atcggttgtg
     7081 gctggggaac attgatgttc actgctacca aagagtataa tgtcaaggca actgggatca
     7141 cactaagtga agaacaatac gatttgatta ccaaaagaat aaaagaagaa catttagaag
     7201 ataaatgccg tgtattatta atggattacc gcgaactaaa aggcgaaacg tttgatcata
     7261 tcacaagtgt aggtatgttc gaacatgtag gttctgaaaa tcttgaaggt tatttcaaag
     7321 tggtaaaaga tttattgaaa ccaaaaggaa cagctttgat ccatggaatc agccgacaac
     7381 aaggcggtgc gacaaatgct tggatcaata aatatatttt ccctggcggt tatgtccctg
     7441 gtgttactga attagttgat aatattacaa aaaacgattt gcaactgatt gatttggaaa
     7501 gcttacgtcg tgactaccaa ttgacattag aacattggac aaagaatttt catcaagtcc
     7561 aagatcaagt cacgaaagaa atgggtgatt ccttctatcg aatgtgggat ctatatttac
     7621 aagcttgtgc agcttcattt gaatcaagta atatcgatgt gattcaatat ttgcttgttc
     7681 aacctggaaa caatgacatc ccaatgcatc gtgcataatt gaggcaaaag tactgtttaa
     7741 agactattta aaaagaagct aagttttatg atgctccaga agtgaacttt tttgtctaac
     7801 ttctaggatt gtctctaaag cttagtttct ttttctttat attttcataa taaaagtttg
     7861 gttctttaat ttttaaacat gacagaaaat tttttcttcg ttatacttgt aatagaatac
     7921 tattttataa caaaggggat cgaatcaatg aaaaaaactg ttatttatac tagtttattt
     7981 gcagctttgt ttttagctgc ttgtcaatca aataccaatc aaggcacatc tgggaatacg
     8041 tcttccagta gcgaacagcg ctcttcacag gtatcggact cctcaacaaa agcaagcaca
     8101 tcttcgtcta gccagacaaa aagttctagt gaagcaagta ctcagtcaga tatcaaagtg
     8161 tctgcagctc gtgcgatcga attgttccaa gataaatacc cagatactgc aattactagt
     8221 ttggaattag atagtgattg gggatcttac ttctacaaga tagaaggtgt cgatgatcaa
     8281 aaagaatacg aagcaagaat tgatgctcaa aatgaaaaaa tggagacaga gaatccagaa
     8341 actctagatc gagatgaaca aaatggtcaa aagaaaaaag aagatggtct ggatgttgat
     8401 aaattgatca cgattgagga agcagcaaaa atcgccgtcg acaaagtagg aagcggtacg
     8461 gcaacagatt gggatttgga taaagattta ggtactactt attgggaagt caaagtaaaa
     8521 gacggcaatc aaagtacatc agtcaagatc aatagccaaa caggcgaagt actgtcaact
     8581 gaacaagatg attaagaatt tcttcggaaa acaagagttg atttttcttt tagtcatgat
     8641 acactagaca aaataacttg tttgaaggag cgatttactt gagtttagaa acgacctttg
     8701 ccaatcatat ctttgccaat cctttgatga atgcatctgg tgtccactgc atgactaccc
     8761 aggaattaga tgaactggct cattcagaag caggagcatt tatcactaag agctgtacga
     8821 tcaatgaacg aaaaggaaat cccgaaccac gctattttga tgttccttta gggagtatca
     8881 attctatggg acttccaaac ttaggttttt cttattatct agagtatgct cttgcgtatg
     8941 aaaaagtgca ggaaaaccaa aatcaaccgt tatttttttc cattgccggg atgagtgtcc
     9001 aggaaaactt ggaaatgctt gagaaaatag aaaaaagtgg gtttaatggg atcactgaat
     9061 taaatttatc ttgtcccaat gtgcctggaa aaccacaact agcttacgat tttgaggcca
     9121 cttatgaaac actgaaagaa gtattcagca tcttttctaa gccgctagga atcaaattgc
     9181 ctccttattt tgattttgct cattttgatc agatggctga tatcttgaat cagtttccat
     9241 tgacctatgt caatgctatc aacagtgtgg gcaatggttt gtatatcgat acagaacaag
     9301 aagcagtagt catcaaacca aaagaagggt tcggcggcat tggcggagag tatatcaagc
     9361 cgactgcttt ggcgaatgtt cgcgcttttt atacacgttt gaaaccagaa atccaaatca
     9421 ttggtacagg tgggattcgg acaggacaag atgcatttga acatctgcta tgtggcgcaa
     9481 gcatgttaca gattggtacc gagcttcata aagaagggcc ggaaattttt tctagaatca
     9541 taaaagaatt aacgcagatt atgtcagaaa aaggatatac gtcaattgac gaatttaaag
     9601 gcaaattacg caccatttca tagcaaagag acgaaggtca tgattgatct tcgtcttttt
     9661 ttataaaaaa cactcgtctg ttcattcctg tgcaagcgta ctcattgtat gttagacgaa
     9721 cttgtgggaa aataacagag gcaaaaagga ggataatatg aatgatgcaa cagaattagc
     9781 aaaagagctt aaagaaaaag aatttagttt tcaagaacta gttacggata tacagaaaaa
     9841 aataaagaag caaaatcatg aattgaatgc gtttgtcgcg cttgaaccat tcgagagtaa
     9901 ttcccacgag ctattgattc cccagtcaga aaaagggcca ttggctggta tcccatttcc
     9961 tttaaaaatg ctcggtcaag aaaaaaaggg atggctagct acttcaggat ctcgtttatt
    10021 tgaaacacac cgagcttctc atacatctaa ttatgtgcgg caagcagaag cgataggtct
    10081 agttcctttt ggacaaacca atgcgccaga gtttggtttc aaaaatatta cagacccagt
    10141 catctatggc ccagcaagaa acccttggaa ccttgaatgt acaccgggcg gatcaagtgg
    10201 aggagctgct gctgcagttt catcaggaat cgttccttta gctggtgcta gtgatggcgg
    10261 tggttctatt agaatacccg cttcgttttg cggattgatt gggctgaaac cttcgcgagg
    10321 gacgatgcca gttggtccat atgcttggag aggatggcaa ggagctgcta ttgatttcgg
    10381 cttgacagtt tctatgagag acacggaggc tttattttat gggatgagaa gtatccattc
    10441 cggtgcgcca tatcaagtat cgccagttga gtggcaaaca catccaagaa aaaaacggtt
    10501 gaagatagct ttttgtgttg attcaccgat ccactcacag attagtgaag aggcagtact
    10561 tgctgtaaaa aaagctgtag atttccttgg aaaagaaggg catgatttga cagagattcc
    10621 ttacccacta gatggtcgta aactgatcac gtcttattat cagatgaatg gcgcagaaac
    10681 agcagcaatg atccgctcga ttgaaacagg catccagcga gctgtcagaa aagaagaact
    10741 tgaacccatt tcttggactt tgttccaata tggtcagaag ctctctgccg ctacttacat
    10801 cgattctctg catgtatggg atgaagcagc aattgtcatg gaaaacctac atcaagaata
    10861 cgatctattc ctttcaccaa cgactgcatt taccgcccct aaaatcagcg aagatttgca
    10921 aagtgatgcg ataagagcac agatggaaca catcagtgat tgttctgaag tagaaggttt
    10981 agacgttgtt tcttccatgt ttgaggagag cttgaagatc acaccttata cacagttggc
    11041 taatctgact ggacaaccag caatcagctt gcctactcat ttgacaaaag aaaacatgcc
    11101 tctaggaatc caatttatgg ctgccagagg aagagaagat ttgctatttc aaatcggcta
    11161 tctatttgaa gcaaatcatc agttccattt accaaatcca tggaaatagc agaaacaagg
    11221 agaagaagaa tgaagaaaaa aagattgttt gctctaataa ctagtgcctt ttttatttta
    11281 agcgcttgcg gtacagctgc cgctaatcag gaagataaca gctggagtaa tgtagaaaaa
    11341 aagaaagaat tagtagtagc gacttctggc acactgtttc ccgcatctta ttataatgat
    11401 caaaatgaat tagttggtta tgatgtagat ttggcaaaag aggtggctaa acggttaaat
    11461 gtcaaaatct cctttaaaga atacaacgtg gatggacaaa tcacttctgt ttcaaaaaga
    11521 gaatcagatt ttgctgcgaa cgattttggt atcacaaaag aaagagaaaa aaaattctcc
    11581 ctttcagaga ctttcaaata ttcatttgac agtatgatcg tacgaaaaaa agatgattca
    11641 ggaatccact cacttgaaga tcttaaaggg aaaaaagcag ctggcgaacc caatactagc
    11701 tatatgcgtt tagcggaaaa acacggagct aaattagtca catatgacaa tgccacgaat
    11761 gatcagtatt taagcgatgt agcaaatggt cgaacagatg ttattctaaa tgattattat
    11821 ctgcagaaaa tggccgtatc tgctttacca gatatccctg taaaaatact ggagaacgtt
    11881 tatttcaatg aaaacgaaag tggtttttta ttgaaagaag gaaatgatgc tctgaaaaag
    11941 aaaatcgatg gcgtattaaa agagatgaaa gaagatggga cgacaaagaa aatatctgaa
    12001 aaatattttc aaactgatgt ttcagtcaaa ccgaaagaaa cgatggctga gtcggtgagt
    12061 gagtgatgta ctttcctcta attgattttt ccctcatgtg gacttcactt cctttcgtac
    12121 tgcaaggctt ggtatacaca ttagggattg gcttcgtgtc gtttctttta ggaaatctgg
    12181 tagggttgct tttgacagtt ttaggtcttt tggattggct gcctctgaat gtatttattc
    12241 gtttttattt atcgttcttt agaggcattc ccgcactagt cctgctgttt ctgttatatt
    12301 ttgggttacc ttatcaatta agtgccttaa cggcttcagt catctgcttt acgattacca
    12361 gcagtgcatt tattggtgaa atctatcgag gatcactagc aggcgtttct tcaggtcagt
    12421 gggacgctgg tttagcttct ggtttttctt tctttggggt attgaaatat atcgttttgc
    12481 cgcaagcttt tcgaatctca gtaccggcac tcagtaacgt agctatggat ctagtgaaag
    12541 gtacttcgtt agcagcaaca atcacagtgc cagaaatatt tcaaaaagca aagatcatag
    12601 gcggtcggac attcgattat ttttctctct atattttagt tgcactgatt tattgggtca
    12661 tttgtatcgg gatcgaaaaa gtgcaacagt atttagaaaa acgttctatg catcagtcaa
    12721 tctgaaaaat agaaatggtg ctagcgtggc tggcaccatt tctatttttt gtttcttgta
    12781 tattctgcct agtattttaa aaccatttca tgtaaaatgt aaatcgaaaa tatgttcgga
    12841 ggtagaaaac atgaagaccc cgctaaccat cgaaatagaa tcctcgttat accaatattg
    12901 catcgagcaa ggcggcttgg ttgtagaaga agtcacgatg cctgatgacc aaggaattgt
    12961 agatacgctt agctgcttga caaaatttga tggaagccga gaatggcgat gctatgagct
    13021 gaaagtgaca aaagcagatt ttcgctctac agccaaactg tcatttgttg gacattataa
    13081 ttattttgtt ttaccaagta aactatatga agaagtaaaa actgaaatcc ctacaggaat
    13141 aggtgtttta gtgtatcgtc cgtacacggt tgaagaggag atgcctgcct ctggtacatt
    13201 tctgattgcg aaaaaagcag caaagaaaga gcttgctgta ccggaagaag ctttgacaaa
    13261 ccgcttcatg gcttctttat tcagggaagt aagaaaagca aagcaaatgg agcgaggcgt
    13321 aaaatatttt ccaacagatc aattgtacaa agaattgaaa agacgttcag aaaatcaaga
    13381 tccattttct aacgaacgat attatgaacg gtttttagag gaaatagcta gtcaggcaat
    13441 caatgagttg caagaagaag ttatcgcgct tcagcatgat tatgaatatt tgaggcaaca
    13501 acagcagatc cgtcggttgc ctacagagcc attggagtga aaaatatgta ctatccttat
    13561 ttaagaggaa aacaatttga cctattagca ttaaaagaag cactgtcgcg aggattgctc
    13621 tccaataaaa tccaaccagt gatcgaacca gtacgtgatt ccgctacgct taaaaatgtg
    13681 atcgaattat ttcaaaaaaa gcaccatcca atcgctgtga tccaaaatcc ccaagtagga
    13741 caattcaaat tattcgatca gcatgtgcac agttggacag tcaaagaaaa tagctctgtc
    13801 gtccctgcac agatcttaac gccagaaaat ctcagtgaag tactggattc acctccagcg
    13861 tttcttgttt tcgatggaca gcactaccca aaagatacag aggtgtggaa acagttggca
    13921 ggagttgatt cgaaattttt gataccagat actagtcgct ttcgcatctg gctgcccgaa
    13981 aacaaaattg tgatccgtga ctcttttcaa acgagaaagc atgttgaaag ttatgcagat
    14041 aaaacagatg acttttttag tgatgactat ctttttttcc acgcagacgg atatattgga
    14101 ttttctgatt tcacgatcga aggtagccgt tatttcgata aagggttccc tagccgagcg
    14161 cttgctatcc atctaacgta tattgatgct tacggcaata tacgcgtgaa acattttgtc
    14221 tctgacagta acgactctgc taaagaccaa gcattgaagt ttcttgaagc aggagataaa
    14281 atgaaaaaat ggatcatgcg tcaccatcat caactcttga ttacttcagg aatgattgaa
    14341 ttattggctc tttaccagca acaaaaattc cctggactgg gagtgttgaa aaagtggtca
    14401 cttatgcatc atttggaact tattagtcac ctgctagatc atccaaaaga ttggcttaaa
    14461 tattattcaa aagttcagga actatatgaa gtgatccaat gattagataa tgtaaatgaa
    14521 aaaggaatag aaaagaggaa actggatgta tattcggcaa acaaggaaag aagaagtaga
    14581 agaagtggta gaaatcatta atgccgcagc ggatttcatt gcttcgcaag gctctcctca
    14641 atggcaaaac gggcagaaac caacaaaaga aacgattttg aacgatattg aaagaaaaga
    14701 aagctatgta ttaatagtgg aagatcaaat tgctggaaca gctgcattag tctcaagcgt
    14761 ggatcccgtt tacacaaaaa tagatggtag ttggaaagaa tcaactggtc cttatttatc
    14821 gattcatcgt gtcgctttag cagaaagtac tcgaggaaaa aaactagcaa aaccgttctt
    14881 agaacatttg ttgacgattg cagccagcaa aaatatccaa gatatccgta tcgatactta
    14941 tccagctaat gaaccgatgg aaaagaccat ctattctgct ggattcactt atcaagggat
    15001 gattgaattt ccttttgaac acggcgaaag aaaagcatat caaataatca tcaattgact
    15061 ataaaatcat ctgagcaaag ataaagacat taacactact gaaaaaaaca atcatatgat
    15121 agccaagagg ttgtaacaga agtggccagc ttcaaaaaac aagaaagaat ttccgaaaat
    15181 gcttctcaaa tttttgtgga attttagcgt attttacgaa atagctgctt tctgaaactc
    15241 ccttcatcga gaggtcgtga catagctttt gtcacgacct ctcggcttat cctacgagta
    15301 ttattcgata catttatatc aataatcctt tttttatttt atctgcagct tcttggaacg
    15361 ctccatcgtt tgacacgact aaaagtgagg ggatagcttt caaacgtttc aactgtgcta
    15421 aatcttctac ataaaccgcc agtctggctg aaatctgttc agatgaatct ccacgttttt
    15481 tcaggcgcga ttgcaatgtt tctttagaga tcgtaataaa gataggacgg actaccgtcc
    15541 cgaaatactc atataaatgc caaaaaccat ttgctgtcaa aacggcgtaa caatttccta
    15601 ctgtcagttt ctttttaatt tctgctttac tggaaccata tttatgttga gcatattggt
    15661 cccactctgc aaactcattt cccttgatca tctgattgaa tgtattgtca gaaacaaaat
    15721 aataatcctc ttgatctttt tccccaggtc ttggtgatcg attcgtgtaa gtaatgatct
    15781 tttgttccgg ctgaaaaata gcttctgcga gtgaagtttt tccagaacca gaaggaccga
    15841 taaatacata gcaataattc attgtcattt ctcctttgat gattgaacaa gtgaaaaagc
    15901 tttggaaaat atttccaaag cttttaaaag taactttgct tttttgtata ttataccagc
    15961 aagccagtca caatagcact taaaaagctg actaaagtcg ctgttagcaa aattttcaat
    16021 ccgtgttttg cgacaagatt tcctttttct tcatttaatc ccttcatcgc accagaaatg
    16081 atcccgatag aagagaagtt ggcaaatgaa acgaggaaga cagatataat tgctgttgta
    16141 cgctcagtaa aatggaaatt accatttgct aattctgtca tagcaacaaa ttcatttgta
    16201 accagtttag tcgccatgat actaccggca tccactgctt ctttccatgg gattccgctg
    16261 atgaaggcaa gaggggcaaa gatgtaacct aggatctctt ggaaagtgat gccgaatatc
    16321 ccgtggaaga tagcattgat catggcaatc aaagcaacaa aaccgataag catagcagca
    16381 accgtgatcg ccacatggaa accatcaaga atgtattctc ccaacatttg gaaaaatgtc
    16441 tgttctttgt tttcttcaat gatcagctca tcttcttttt cttctaatac atagggattt
    16501 acaattgaag ccagaatata accgccaaat aagttcaaaa caagggcagt gatcacatat
    16561 ttagaatcga tcagtgccat ataagatccg acgatcgaca tagaaactgt agacatagcc
    16621 gatacactca tggtgaacaa acgtttctca gataggaagg gtaattcctt ttttatggaa
    16681 ataaaaactt ccgattgtcc taaaatagca gaagctactc cattataaga ttccaatttg
    16741 cccatcccgt tgattttact taatcctaga ccaacagctt tcataaataa tggaagaatc
    16801 ttgatatagc gtagaatacc gataatagca gaaataaata caattggcat caatacggaa
    16861 agaaagaaag gtgcacttcc tttatttgct acaccggcaa aaacaaagtc aacaccttgc
    16921 ccagcaaaag caagaagatg atcaaacatt tttgccaata cagaaacaac cgctgtacca
    16981 aaagttgtac gtaataatac gaaaccaaga acaagctgta aaacgaacat cacaattagt
    17041 ggtttcaatt tgattgcttt tttgttactg ctgatcaaaa aagcaagccc taatgtgaca
    17101 aataaaccaa taatacctaa taaataactc aaataatcac tcctattcca aattcc
//
LOCUS       DACDKM010000053        17070 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20834_2016
            SAMN12640370-rid9891663.denovo.053, whole genome shotgun sequence.
ACCESSION   DACDKM010000053 DACDKM010000000
VERSION     DACDKM010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640370
            Sequence Read Archive: SRR10040891
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17070)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17070)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742835.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742835.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:04:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,012
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 29 of 75
            Pseudo Genes (incomplete)         :: 33 of 75
            Pseudo Genes (internal stop)      :: 19 of 75
            Pseudo Genes (multiple problems)  :: 5 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17070
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640370-rid9891663.denovo.053"
                     /isolate="E20834_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(<1..626)
                     /locus_tag="IV334_001626"
     CDS             complement(<1..626)
                     /locus_tag="IV334_001626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAP8943697.1"
                     /translation="MDNHTRKLLGLTDKHLFFDEEWLIEKEYKGVQAQFIKGKLDDSS
                     SYCEHCRSVRIIRNGSYTTRTQMLKVKEKLTILELKRTRFLCHDCGRTFSAKTDLVDE
                     HHQLTKELKQVILMELYENQSRKLIAKKYFVSDGTVTRILREATKHYQPRMNFLPTVL
                     CMDEFKSMNSVSGSMSFICVDGTTNQLFTILEDRRLYKLTQYFLRFPR"
     gene            899..3370
                     /locus_tag="IV334_001627"
     CDS             899..3370
                     /locus_tag="IV334_001627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HAP8943698.1"
                     /translation="MKNKWINRSKKKKVTQRSSDKVIRLFPSYKEGLTADQVKERIEK
                     GAANNSVDPTFKTNQQIVLENIFTYFNLIFLILAILLCLVESYKNLTFLPVIIANTGI
                     AIFQEIRSKKILDELNVLNTAKVNVIRDGIEQTIDIEDLVIDDIVILETGYQIPADAE
                     VVEGKLQVNEALLTGEADEITKEIGDPLMSGSFIVSGKAYARLDKVGADSYISQLTVK
                     AKTMGEGEQSEMIASLNQLIKWIGIIIIPIGILLFSQSYFFNGNTIKESVVAMEAALI
                     GMIPEGLYLLTTIALALSATKLAKQRVLLHNMKSIETLARVNVLCVDKTGTITENKMS
                     VQKMIVSKKQEIISQSDQLEACIADYAKAMAGDNATMEAVKAYFTKTTDQSYHHVIPF
                     SSVQKFSSVALADKVYVLGAPEMVLRDQLPEYAEEFVSFAEKGYRVLVFGVYDGILEE
                     PVLTEAVTPLGYILIANPIRKEARATFDYFKKQQVAIKVISGDNPLTVSNVAVQAGIS
                     HADQYVDASKLSEEEYEAAVEKYTVFGRVKPEQKKIFVQLIKRNNTVAMTGDGVNDIL
                     AMKEADCSIAMASGNEAAMQASQVVLLDSDFSRMPEVVAEGRRVVNNIERSASLFLVK
                     NIFSFLLSLFSVIFALTYPLEPSQITLISLFTIGLPSFLLALEENKKRIRGKFIMNVM
                     EKAVPGGLTDMIVVGALVICGVTLNLNKTDVSTASTMLLIAVGFLVLYKICSPLNKFR
                     SQIILFCASGNFFSVIFLHKLFSITGISAVSLLLLSILFFSADSIFRHLTTLVEYLFE
                     RKNEKEQKPFSRVAKKFLSIFKREN"
     gene            complement(3473..5209)
                     /locus_tag="IV334_001628"
     CDS             complement(3473..5209)
                     /locus_tag="IV334_001628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component sensor histidine kinase"
                     /protein_id="HAP8943699.1"
                     /translation="MKWKKIYERLFFLILLVSLFFGGWQLISHYFQQQIVEQQESYLT
                     KKAQLLIRQLDVENLQAAQNKTALEEFVHQSNERITLMDATGKILFDTNNETLNEQRN
                     SRPEIKAVLNGGNLGSALRKSTTLNEDLLYVALPVKKSGQLEAILRIAEPTSGFLPRT
                     ESFRRWVFFFFLSFFLVLAGMIYYLIYQKNQPLKTVLPVLKKMVQNPNQAEVIMQTPD
                     QWEELYQTINALSEQMSKMYRACTTTEEQLYTLLNELMIGVFLIDDADSRLLLLNPKM
                     QLHLNVVSYQPEQRYTEVIQEPKLIQLIHQVTPEHPLIHQEITLTEGRQTLDLSLRYF
                     TNSEGTGQILGVAYDLTKVRRLEKLQKDFVGNVSHELKTPVTSLIGFTETLLDGAKDD
                     PQTLTSFLEIMQKDAIRLDKLIREIIQLSKDGENSYEIQTIYTEPYFQQIVQNYQPII
                     EKKRLTIRLIGKNEPFTTKTDILYPIIKNLIENAVQYSKSDSEIIIRYQATDDLSFSV
                     QDFGIGIDIEDQERIFERFYRVDKARSRHSGGTGLGLAIVKDYVQLLNGTITVDSHLG
                     TGSTFTVTIPKM"
     gene            complement(5206..5910)
                     /locus_tag="IV334_001629"
     CDS             complement(5206..5910)
                     /locus_tag="IV334_001629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP8943700.1"
                     /translation="MKKILVVDDEPSIVTLLTFNLEKEGYKVTSATDGGEGLELALEQ
                     SFDFIILDVMLPTMDGMEITQRLRQEKNETPILMLTAKDDQVDRIIGLEIGADDYLTK
                     PFSPREVLARMKAIFRRIEPRKQSKDEAEPEYLSIGQITADLTNYQVTVDDHLIELTP
                     KEFELLVYFMKRKDRVIDRDTLLDRIWNFDFAGQSRIVDVHVSHLREKIERDPKHPKY
                     LVTIRGFGYKFQEPKR"
     gene            6041..6544
                     /locus_tag="IV334_001630"
     CDS             6041..6544
                     /locus_tag="IV334_001630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF523 domain-containing protein"
                     /protein_id="HAP8943701.1"
                     /translation="MIGISACLGGLACRYDGQKKEVPELMELVETGEAVMVCPEVIGG
                     LPIPRDPAEIIGGNGFDVWKDQAKVWTTSGEDVTEAYKNGAIKAYQKLQEKHIDLVIM
                     KEKSPSCGTHSIYDGSFSGIKKNGAGVATAYFIQQKMTVLSDEEWLKTRGEQNGNRKD
                     QSNERAL"
     gene            6504..6842
                     /locus_tag="IV334_001631"
     CDS             6504..6842
                     /locus_tag="IV334_001631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding protein"
                     /protein_id="HAP8943702.1"
                     /translation="MEIEKTNRMNALFEFYSTLLTEKQMNYMELYYADDFSLGEIAEE
                     YEVSRQAVYDNIKRTSKILEDYEKKLHLFSNYIVREQVLENMTAYITDKYPEDKKLLE
                     YVQQIQAIEE"
     gene            6863..8281
                     /gene="ffh"
                     /locus_tag="IV334_001632"
     CDS             6863..8281
                     /gene="ffh"
                     /locus_tag="IV334_001632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HAP8943703.1"
                     /translation="MAFESLTERLQQAMGKIRKKGKVSEADVKEMMREIRLALLEADV
                     NLQVVKDFTKRVRERAVGAEVLESLSPAQQIVKIVDEELTITLGSETAELNKSPKIPT
                     VIMMAGLQGAGKTTFTGKLANYLKKNENARPLLIAGDVYRPAAIDQLKVLGQQLDVPV
                     FDMGTEVSPVEIVRQGMELAKEKKNDYVLIDTAGRLHIDETLMDELKQIKELTQPNEI
                     LLVVDAMTGQDAVNVADSFNQQLGITGVVITKLDGDTRGGAALSIRSVTGAPIKFIGS
                     GEKLTDLEVFHPDRMASRILGMGDMLTLIEKAQQDYDEKKAEELAQKMRENSFDFNDF
                     IEQLDQVMGMGPLEDLIKMIPGMNQVPGIENVKVDPKDVERKKAMVYSMTPAERANPD
                     LLNPSRRRRIAAGSGNSVVEVNRMIKQFKESRKMMQQMSKGDMNIPGMDQMFGSGVKG
                     KLGKMAMNRMIKKNKKKKKKRK"
     gene            8393..8971
                     /locus_tag="IV334_001633"
     CDS             8393..8971
                     /locus_tag="IV334_001633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HAP8943704.1"
                     /translation="MRCPWSEQTESMRQYHDTVWGVPEYDDQKLFRKLMLDINQAGLS
                     WQTILNKSEAFDEAYDRFDIKKVANYGETKISELMENKGIIRNRRKIEAAVQNAKAVL
                     KIQEEIGSFSEYLWSFSNGKIVDSSYHSQEEVPTTSPLSDELAKDLKKRGFKFIGSTT
                     IYAFLEAVGIINDHLDSCFRKEEVVRIGRKGE"
     gene            8975..9496
                     /locus_tag="IV334_001634"
     CDS             8975..9496
                     /locus_tag="IV334_001634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HAP8943705.1"
                     /translation="MRKIIFYGAISLDGYLATKTDDLQWLFDTPTGKQTTYEAFYETI
                     DTTIMGRKTYQEAKKYLETEAIYPEKKNFVFSTHAQLQLPDAEVVNEDPVAFLKNLQM
                     TEGKDIWVVGGGRLIKPLLEHQMIDEWYIQITPVLLGDGIPLFQKGTYEARFEMLDTT
                     RFGEFIELHYVRK"
     gene            complement(9575..10129)
                     /locus_tag="IV334_001635"
     CDS             complement(9575..10129)
                     /locus_tag="IV334_001635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HAP8943706.1"
                     /translation="MTKKIGFFIGSLRKDSYNKKVAEAFAKLLPEGYESVFVKIDDLP
                     FYNEDLETPEQAPAEWTRFREEVRGLDGVVFVTPEYNRSVPAVLKNALDVGSRPYGHS
                     VWDHKPGLVVTASPGGIGGFGANHHLRQSLVFLNVPTLQQPEAYIGNVANLIDENGNI
                     VEGTLSFFQTILDAYLDFSNKLTE"
     gene            10382..10657
                     /gene="rpsP"
                     /locus_tag="IV334_001636"
     CDS             10382..10657
                     /gene="rpsP"
                     /locus_tag="IV334_001636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HAP8943707.1"
                     /translation="MAVKIRLKRMGSKKSPFYRIVVADSRSPRDGRFIETVGTYNPLK
                     DPAEVVLKEDLVLDWLSKGAQPSDTVRNILSKEGVMQKHHEAKFSKK"
     gene            10669..10920
                     /locus_tag="IV334_001637"
     CDS             10669..10920
                     /locus_tag="IV334_001637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HAP8943708.1"
                     /translation="MKDLSDLVLTIVRPLVTYPDQVKLDVVESKDFYEYNLTVAPEDI
                     GRIIGKQGRVAKAIRTIVYGVRINAPKKVRLNIIDDKEK"
     gene            11045..11836
                     /locus_tag="IV334_001638"
     CDS             11045..11836
                     /locus_tag="IV334_001638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8943709.1"
                     /translation="MRKKMLFIPFALLLSACSSVTQESDNTHLSSSISETVTSPEKSS
                     TKTASTTEQTMATSNNEKKTALDQLKEQQPNVPMPLDVPVSSGYLNIAATHTKQGYSI
                     LYYRTDRPLGLNADELNQETPIATYLYQYGFASSQETIQVLQPFEIDTNGQQVDLGSR
                     ITGYQQGAAGSSFLEWQEGNWRIRIRGNNIEGQDPLLLAKEIVAYLEENSLPAPEQFG
                     KITVDMGDTTNRAVEVSWQEPKNAYTITHQDPMSALKMAVSMKRL"
     gene            12006..12527
                     /gene="rimM"
                     /locus_tag="IV334_001639"
     CDS             12006..12527
                     /gene="rimM"
                     /locus_tag="IV334_001639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HAP8943710.1"
                     /translation="MTEYLNVGKIVNTQGIKGEVRVISTTDFPEERYKKGTVLTLFQD
                     GKAPVELTVKSHRKHKNFDLLSFEGHPSINDVEKYRDGILKVSKDNSVDLAENEFYYH
                     EIIGLHVLEDGTELGKVKEILSPGANDVWVVQRPKKADILLPYIASVVKEVDLEAGVV
                     RVEIPEGLIDDED"
     gene            12517..13278
                     /gene="trmD"
                     /locus_tag="IV334_001640"
     CDS             12517..13278
                     /gene="trmD"
                     /locus_tag="IV334_001640"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HAP8943711.1"
                     /translation="MKIDVLTLFPRMFEGPMGESIIGKAVDKGLLDINISNFRDYSDN
                     KHQTVDDYPYGGGAGMLLKVQPIYDNIQAIEKAAPDVKKRVILLDPAGKRFDQKMAEE
                     FSQEEHLVFICGHYEGYDERIRTLVTDEVSLGDYVLTGGELGAMVMIDATVRLLPDVL
                     GNQTSAQTDSYSTGLLEHPQYTRPAEYKGMKVPEVLTNGNHKLIEEWQLKESLRRTYQ
                     RRPDLLETLEWTPQMTKFLEEIKKEEQEKTADEAK"
     gene            13414..13761
                     /gene="rplS"
                     /locus_tag="IV334_001641"
     CDS             13414..13761
                     /gene="rplS"
                     /locus_tag="IV334_001641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002357176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HAP8943712.1"
                     /translation="MNPLIEELTKEQLRSDIPAFRPGDTVRVHAKVVEGTRERIQLFE
                     GVVIKRRGAGISETYTVRKVSNGVGVERTFPLHTPRVAKIEVVRYGKVRRAKLYYLRA
                     LHGKAARIKEIRR"
     gene            complement(13893..15029)
                     /locus_tag="IV334_001642"
     CDS             complement(13893..15029)
                     /locus_tag="IV334_001642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAP8943713.1"
                     /translation="MWIETKTDKNGKKVYKYNERYIDPKTRKRKKVSITYKNKSRETQ
                     KVALLELNKKIDIKLNEKTLQKPDLTFHELVEEWLVIYKRQVKESTYYPTNNILNTIK
                     KKIPETYIVSGINTVDLNNIFEDMIYKDDLSNKYVSVIKSKLNLLFSYAMKKGYVEKN
                     PINEVVIDYKRESKTIKIKDKFLEDDEYKRLVNFTTSHNKRYSLLFQWLYLTGMRPGE
                     AIALSKDDVHITNNNASVVINGTMMYRERSIADMKKSDSTKTAAGMREIDLPKKAIAI
                     YNELLELNPNGQFLFQTTKGTPFQLTAINTYLRNHKADMKIDKKLSSHIFRHTHISKL
                     AELGTPLYAIQDRVGHENSDITEKIYLHVTKGVKEKLKEDIEKL"
     gene            complement(15145..16059)
                     /locus_tag="IV334_001643"
     CDS             complement(15145..16059)
                     /locus_tag="IV334_001643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4352 domain-containing protein"
                     /protein_id="HAP8943714.1"
                     /translation="MKKIVFGLLFLGLVLSGCSTSPNSASTKENTVSSTSEYETKKLT
                     SSDVHKIKVGDDLTQVYQTLGSPLRKWTNDFVYKELDNAITRDELIVSISKDAQGDYY
                     KEHSELIEHGNSAKDIKKLEMLQYTYSTDSDDETMLIWVSPKTEQVVYYNQRTFLDKD
                     GKSKDSSSTTTTTTTSSSEPQTASVAHSIGEAVPFTSENGNSINVTVQSVSKDYGDDF
                     YKPEGLFYAKVDFLVENTGTTPFDASSSYLEFYDSSDIKSDLASRDYFSETIQPGKSA
                     QGTAYFEVKNDGTTFEVFFADTSWKGSY"
     gene            complement(16150..16578)
                     /locus_tag="IV334_001644"
     CDS             complement(16150..16578)
                     /locus_tag="IV334_001644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HAP8943715.1"
                     /translation="MEMDTINLVEELKRKYQSANPFYICEKMGIKIQYVPFIENPKGQ
                     FQEIRGRAIIFLNDELRDSEERFYICAHELGHAIFHRGLSSYYVSTRTSRSKSESEAN
                     CFAANLIVSLYKEDNDQYPKRIEELKNLYGLPESAYRFLI"
     gene            complement(16583..16957)
                     /locus_tag="IV334_001645"
     CDS             complement(16583..16957)
                     /locus_tag="IV334_001645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP8943716.1"
                     /translation="MNLLDRIKELAHKRGISITQLEEELNIPKNTIYQWKNRTPSTKR
                     LQLVADYFNVTTDYLLGRNQVPDWATKDEVVELDKLLDSNVNMSYGGETLTPEQVQRV
                     KDILIGTFWDIVKEDKEKGKKM"
BASE COUNT         5488 a         3086 c         3352 g         5144 t
ORIGIN      
        1 tttctaggaa agcgcaggaa gtattgagtt agtttgtaaa gtctgcgatc ttcaaggatc
       61 gtaaatagct gattggttgt cccatcaaca caaataaaac tcatggaacc agaaacagaa
      121 ttcattgatt tgaactcatc catacacaac accgtcggaa ggaagttcat acgtggctgg
      181 taatgcttcg ttgcttcacg caagatccgt gtcaccgtcc catccgagac gaagtacttc
      241 ttagcgatta gtttacgtga ttgattttcg tacagttcca tgagaatcac ctgcttcagt
      301 tcctttgtta gttgatgatg ttcatcaact aagtccgttt tcgcagaaaa ggtccttcca
      361 caatcatggc agagaaaacg ggtacgtttt aattctaaaa tagttaactt ttctttgact
      421 ttcaacatct gagtgcgtgt cgtataggaa ccgttgcgga taatacgaac cgatcgacag
      481 tgttcacaat aggaagaaga gtcatccaat tttcctttaa taaactgcgc ttgaacgcct
      541 ttatattctt tttcaatcag ccattcttca tcaaaaaata aatgtttatc tgttaatccg
      601 agtaattttc tagtatgatt atccattgag agactccttt ggtaggatga cgttttggtc
      661 gactaaattt taccaaaagt gagtctcttt ttgagttgaa acgaagaaaa agtgttagta
      721 gaaaatcgcc ttcgattttc caccaacaca aaatattata gaaccctttt tttttgctat
      781 atgacaggaa tgaaaggtgt ttgcaagctt aatgtgagga acactttcca tatctgttct
      841 atactggata ggagaagttt ggaaatatcc agaaaacgaa atcaatcgag gtagccacat
      901 gaagaataaa tggataaatc gaagtaaaaa aaagaaagta acacaaagat catcagataa
      961 agtcatccgt ctttttccat cgtataaaga aggattaacg gcagatcaag tcaaggaacg
     1021 aatagaaaaa ggtgcagcaa ataattcagt cgatcccaca ttcaaaacaa atcagcaaat
     1081 cgtattggaa aacatattta cgtatttcaa tttgatcttt cttatcttag ccattttgct
     1141 ttgtttagta gaatcctata agaacttgac ttttttacca gttatcatag ccaatacagg
     1201 aatcgccatt tttcaggaaa ttcgatccaa aaagatcttg gacgaactaa atgttttaaa
     1261 tacagcaaaa gtcaatgtca tacgagacgg gatagaacaa acaatagata tagaagacct
     1321 tgtaatagat gacattgtta ttttagagac tggatatcaa attccagcag atgcagaggt
     1381 cgtggaaggg aaactgcaag ttaatgaggc tttattgaca ggcgaagcag atgaaataac
     1441 caaagaaata ggtgatccat taatgtccgg cagtttcatt gtttccggaa aagcttacgc
     1501 ccgattagac aaagttggag cagattcgta tatttcccag ctgacagtca aagccaagac
     1561 aatgggagaa ggagaacagt cggagatgat tgcttctctt aatcaattga tcaaatggat
     1621 tgggatcatc attattccta tcggtatcct gcttttttct caaagttact tttttaatgg
     1681 aaacacgatc aaagaaagtg tcgttgcaat ggaagcagca ttgatcggta tgatacctga
     1741 aggactgtac ttgctgacga cgattgcatt agcattaagt gcgactaaac tagcaaagca
     1801 aagagttctt ttacacaata tgaaaagcat tgagacattg gcaagggtaa atgtcctttg
     1861 tgtagataaa acaggaacga tcacagaaaa taaaatgtct gttcaaaaaa tgatcgtctc
     1921 taaaaaacaa gaaataatca gtcagtcaga tcaattggaa gcatgcattg ctgactatgc
     1981 aaaagcgatg gcaggagaca atgcaacaat ggaagctgtt aaagcatatt ttacaaaaac
     2041 cactgatcaa tcctatcacc atgttattcc tttttcttct gtccaaaaat tcagcagtgt
     2101 agcgttggca gacaaggtgt atgttttagg agcgcccgaa atggttttgc gcgatcaatt
     2161 gccggaatat gcggaagagt ttgtttcttt cgctgaaaaa ggctatcgcg tgttggtctt
     2221 tggtgtgtac gatgggattc ttgaagaacc agtattgaca gaagcagtca caccattagg
     2281 ctatattctg attgctaatc ctatcagaaa agaagcaaga gccacatttg attacttcaa
     2341 aaaacagcaa gtagccatca aagtgatttc tggagataat ccgctcaccg tatccaatgt
     2401 agctgtccaa gcagggattt cacatgctga tcagtacgtt gatgcatcta aattatcaga
     2461 agaagaatat gaagcagctg tcgaaaaata tacagttttt ggacgcgtca agccagaaca
     2521 aaagaaaatc ttcgttcaat tgataaagcg taacaataca gtagctatga caggtgatgg
     2581 cgtgaatgat attctagcca tgaaagaagc agactgcagt atagcgatgg cttccggaaa
     2641 tgaagccgcc atgcaggcct cacaagtggt cttacttgat tctgactttt cacgcatgcc
     2701 agaagttgtt gcagaaggac ggcgcgttgt gaacaatatt gagcgatcag ccagcttgtt
     2761 tttagtgaag aatattttct cctttttgct ctcactgttt tcagtgatct ttgctctgac
     2821 ctatccttta gaaccatcac aaatcacatt gattagtttg tttactatcg gacttccatc
     2881 tttcttgctt gctctagaag aaaacaaaaa gcgaatcaga gggaaattca tcatgaatgt
     2941 gatggaaaaa gctgtgccag gtggactaac agatatgatc gtagtcggtg cgttggtgat
     3001 ctgcggggtg actttgaatc taaataaaac agatgtttct acagcatcta ccatgctttt
     3061 gattgcagta gggttcctcg ttctctataa aatctgttca ccgctgaata aattcagaag
     3121 tcagatcatt cttttctgtg ctagcggtaa ctttttttcg gtcatctttt tgcataagct
     3181 gttttcaatc accggtatct cagctgtatc acttttgctg ttgagcattc tatttttctc
     3241 ggcagattcg atttttaggc atttgaccac attggtagaa tatttgtttg aacgtaaaaa
     3301 tgaaaaagag caaaagccgt ttagtcgcgt agctaaaaaa tttctatcga tatttaaacg
     3361 agaaaattga cgaaaagtat ctcaaaaaag agtaaaaaag aacaaccact cgtgtatctt
     3421 tcgaagcagg atgagtcatc caactttaaa acgcacgagc agttgctctt ttttacattt
     3481 tcggaatggt aacggtgaaa gtcgaacctg ttccaagatg actgtctaca gtaatcgtac
     3541 cattaaggag ttgcacgtag tcttttacaa tcgctaaccc taatccggtc ccgcctgaat
     3601 gtcggcttcg cgccttgtct acgcggtaaa aacgctcaaa gatccgttcc tgatcctcaa
     3661 tgtcgatacc tataccaaag tcttggacag aaaacgatag atcatctgtt gcctgatagc
     3721 ggataataat ctcgctgtcg ctttttgaat attggacagc attttcaatt agatttttga
     3781 taattggata aaggatatct gttttagtag taaatggttc gttctttcca atcaatcgaa
     3841 tcgtcaaccg tttcttttcg atgattggct gataattttg aacgatttgc tggaaataag
     3901 gttctgtata aattgtctgg atctcgtaag aattctcgcc atcttttgac agctggatga
     3961 tttcccgaat cagtttatct aaccggatcg catctttttg catgatctca agaaaagaag
     4021 tcagagtttg cgggtcatct tttgcaccat ccagtaaagt ctcggtaaaa ccaatcagtg
     4081 aagtcacagg agtttttaat tcgtgagaga cgttgccaac gaaatccttt tgtagctttt
     4141 ctaaacgtcg cactttcgtt aagtcatacg ccacccctag aatctgtccg gtaccttctg
     4201 aattagtaaa gtagcgcaaa ctcaagtcta atgtctgccg accttctgta agggtgatct
     4261 cctgatggat cagcggatgc tcaggagtca cttgatgaat cagctgaatg agttttggct
     4321 cttgaatgac ttctgtgtac cgctgttcag gttgatagct taccacgttt aaatgtagct
     4381 gcatctttgg attaagcagt agtaaacgag aatcagcatc atcaatcagg aatacgccaa
     4441 tcatcagttc gttcagtaac gtatataatt gctcttctgt ggtagtacac gcccgataca
     4501 tcttactcat ttgttcgctc aacgcattaa tcgtctgata caattcttcc cattgatcgg
     4561 gtgtttgcat gattacttcc gcctgattag gattttgcac catttttttt agtacgggca
     4621 gcacggtttt caatggctgg tttttttgat agatcagata atagatcatt cctgcaagaa
     4681 caaggaagaa agacagaaag aagaaaaaga cccaacggcg aaaactctca gttctcggta
     4741 aaaaaccgct tgtcggttct gcgatacgta aaatggcttc taactggcca ctttttttga
     4801 ctggtaatgc gacatataaa agatcctcat tcaaagtagt acttttgcgc aaagcgctac
     4861 ctaggttccc accattcaat acggctttga tttcgggacg ggagtttcgc tgttcattca
     4921 atgtttcatt gtttgtgtca aataagatct ttccagtagc atccattaaa gtaatccgtt
     4981 cgttcgactg atggacgaat tcttccaaag cagttttgtt ttgcgctgct tggaggtttt
     5041 cgacatctaa ttgccggatc agcagttgtg ctttttttgt caggtagctt tcttgctgtt
     5101 ccacgatctg ctgttgaaaa taatgggaaa tcaattgcca gccaccaaaa aacaaactaa
     5161 ctaaaagaat aagaaagaag agacgttcat aaattttttt ccatttcatc gtttgggttc
     5221 ctgaaactta tagccaaatc ctctaatagt caccaagtac tttgggtgtt ttggatctcg
     5281 ctcgattttt tctcgcaaat gactaacgtg tacatctacg attcggcttt gtcctgcgaa
     5341 gtcaaaattc caaatgcggt ctagcaatgt gtctcggtcg atcacacggt cttttcgttt
     5401 catgaaataa acaagtaatt caaattcttt cggtgttagt tcaatcagat ggtcgtctac
     5461 ggtcacttga taattggtca aatcagcagt gatttgtcca atcgatagat attcaggctc
     5521 agcttcatct tttgattgct tacgaggttc aattcgtctg aatattgctt tcatacgggc
     5581 taatacttcg cgaggactaa atggtttggt caaatagtcg tcagcaccta tttctaggcc
     5641 aatgatccga tccacttggt catctttagc cgtcagcatc aaaatcggtg tttcattttt
     5701 ttcttgacgc agccgctgag tgatctccat gccatccatc gttggaagca tcacatctaa
     5761 aatgatgaaa tcaaatgatt gttctaatgc taattctaat ccttcgcctc catctgtggc
     5821 actggttacc ttgtagcctt ctttttccaa attgaacgtc aagagcgtta caatagaagg
     5881 ttcatcatca acaactagta tttttttcat tcaaatctcc tttaaatagg tttcattctg
     5941 tttatatttt agcatagaat cagtacaaag ggctatttta caaaagaaat aaatggtata
     6001 ataggaaggc actttgctga atagaaagga agagcagtca atgattggaa tcagtgcatg
     6061 tttaggcggt ctggcctgtc gctatgatgg acagaaaaaa gaagtaccag aattgatgga
     6121 actagttgaa actggagaag ctgtcatggt ctgtccagaa gtgatcggcg gattaccaat
     6181 tcctagagat cccgctgaaa tcattggtgg aaatggattc gatgtgtgga aagatcaagc
     6241 aaaagtatgg acgacaagcg gcgaagatgt aacggaagcg tacaaaaatg gcgcgatcaa
     6301 agcctatcaa aaattgcagg aaaaacacat tgatctagtc atcatgaagg aaaaaagccc
     6361 ttcatgcgga acccattcga tttatgacgg aagtttttcc ggaataaaaa aaaatggtgc
     6421 cggagtggct actgcttatt ttattcagca aaaaatgact gtgctctctg atgaagaatg
     6481 gctgaaaact agaggtgagc agaatggaaa tcgaaaagac caatcgaatg aacgcgctct
     6541 ttgaatttta ttcgacctta ttgacagaaa aacaaatgaa ctacatggaa ttatattatg
     6601 cagatgattt ttcattaggt gagatcgcgg aagaatacga agtaagccga caagcagtat
     6661 atgacaatat caagcggaca agcaagatct tggaggatta tgaaaaaaaa ctccatcttt
     6721 tttctaatta tattgttcga gaacaggtat tggaaaatat gactgcatat attacagata
     6781 agtacccgga agataaaaaa ctacttgaat atgtacagca aattcaagca atcgaagaat
     6841 aaataaaagg aatgaagaaa atatggcatt tgaaagctta actgaacgtc tccagcaggc
     6901 gatgggaaaa ataagaaaaa aaggaaaagt atcagaagct gacgtaaaag aaatgatgcg
     6961 ggaaatccgc cttgccttac tagaagccga tgttaacttg caagtagtca aagattttac
     7021 taagcgcgta cgcgaacgtg cggtaggggc agaagtgctg gaaagtttat cacctgcaca
     7081 acaaatcgta aaaatcgttg atgaagaatt gacgatcaca cttggttctg aaacagcaga
     7141 gttaaataaa tcaccaaaaa taccaactgt tatcatgatg gctggtcttc aaggggcagg
     7201 taaaacgacc tttactggta aattagccaa ttatttaaag aaaaatgaaa atgctcgtcc
     7261 gctcctaatc gcaggggacg tatatcgacc agctgcgatc gaccaattga aagtattagg
     7321 tcaacagcta gacgttcctg tatttgatat gggaactgaa gtcagcccgg tagagatcgt
     7381 ccgtcaaggg atggagttag caaaagaaaa gaaaaacgac tatgtattga tcgatacggc
     7441 aggacgtctg catatcgatg aaacattgat ggatgagttg aaacaaatca aagaattgac
     7501 gcaaccaaat gagattttgc ttgttgtcga tgccatgact ggtcaagatg ccgtcaacgt
     7561 agctgatagc ttcaaccagc aattaggtat tacaggggtc gtcatcacta aacttgatgg
     7621 ggatactcga ggcggggcag cactatcgat ccgctcagtg acaggcgcac caatcaaatt
     7681 catcggttct ggtgaaaaat tgactgactt agaagtattc cacccagatc gtatggctag
     7741 ccgtatcctt ggtatggggg atatgctgac attgattgaa aaagcgcagc aagactacga
     7801 cgagaaaaaa gcggaagaac ttgcacagaa aatgcgggaa aattcattcg atttcaatga
     7861 ctttattgaa cagcttgatc aagtgatggg tatgggccca ctggaagatc taatcaaaat
     7921 gatcccagga atgaatcaag tccctggaat cgaaaacgtc aaggtagatc caaaagatgt
     7981 tgaacgcaaa aaagcgatgg tttattctat gacgccagca gaaagagcaa atcctgatct
     8041 gttgaaccca agccgtcgtc gccgaattgc tgccggttct ggaaacagtg ttgttgaagt
     8101 gaatcgaatg atcaaacaat tcaaggaatc aagaaaaatg atgcagcaaa tgtccaaagg
     8161 ggatatgaac atcccaggta tggaccaaat gtttggttca ggcgtcaaag gaaaactagg
     8221 caaaatggcg atgaatcgaa tgatcaagaa aaataagaag aaaaagaaaa aacggaaata
     8281 agatgaaaaa aacagcttcg gtacagactg aaaatgtcta ctgccggagc ttttctatca
     8341 gcaattttta ttctttacta atacaattac tctaaacgaa gaggtggcac agatgagatg
     8401 tccgtggagt gaacaaacag aaagtatgcg gcaatatcat gatactgtct ggggtgttcc
     8461 tgaatatgat gaccaaaagc tgttccgaaa attgatgctt gatattaatc aagccggatt
     8521 gagctggcag acgattttaa ataaaagtga agcattcgat gaagcatatg atcgattcga
     8581 tatcaaaaaa gtcgcaaatt atggagaaac aaaaatcagt gaactaatgg aaaacaaagg
     8641 aattattcgt aaccgaagaa aaatcgaagc agccgtccaa aacgccaaag cagtcttgaa
     8701 aattcaagaa gaaatcggca gtttttcaga gtatctttgg tcgttttcta atggaaaaat
     8761 cgttgattct tcttatcatt cacaagaaga agtcccaaca acaagtcctc tttcagatga
     8821 attagcaaaa gatctaaaga aaagaggatt taaatttatc ggaagcacaa caatctatgc
     8881 ttttttagaa gctgtcggga tcatcaatga tcaccttgac agttgtttcc gcaaagaaga
     8941 ggtcgtacgt atcggcagaa agggcgaata aagcatgcga aaaatcatat tttatggtgc
     9001 tataagttta gatggctatt tggcaacgaa aacggatgat cttcaatggc tttttgacac
     9061 tccaacaggt aagcaaacta cttatgaagc tttttatgaa acaatcgata cgaccatcat
     9121 gggcagaaaa acttatcagg aagcaaaaaa atatttggag accgaagcaa tttatccaga
     9181 gaaaaagaat ttcgtttttt ccacccacgc acaattacaa ttgccagatg ctgaggtcgt
     9241 aaacgaagat cctgttgctt ttctaaaaaa tctacaaatg acagaaggaa aagatatctg
     9301 ggttgtgggt ggcggtcgat tgatcaaacc gctattggaa catcaaatga ttgatgaatg
     9361 gtatatccag attacccctg tattattagg ggacgggatt cctttattcc aaaaaggaac
     9421 atatgaagcg cgttttgaaa tgctagacac gacacgattc ggtgaattta tcgaattgca
     9481 ttatgtacga aagtagaagt aaataaaata aaaaaggcaa ctatatgcag aaatgtcctg
     9541 tacatagttg ccttttttat taccgttatg ctttttactc tgttaatttg ttagaaaaat
     9601 ctagatacgc atctaaaatc gtttggaaga aacttaatgt tccttcaacg atattaccat
     9661 tttcatcaat tagattcgca acgtttccga tatatgcttc cggttgctgc aaggttggca
     9721 cattcaaaaa gacaagtgat tggcgtagat ggtggtttgc gccaaagccg ccgatcccac
     9781 ctggtgatgc agtaacgact aaacctggtt tatgatccca aacactgtgt ccataaggtc
     9841 ttgagcctac atctaaagca tttttcaata ctgctggcac agaacggttg tattcaggag
     9901 taacgaaaac gacaccgtct agtcccctca cttcctcacg aaaacgagtc cattcagcag
     9961 gagcctgttc tggagtttcg agatcctcat tgtaaaatgg cagatcatcg atttttacaa
    10021 atacactttc gtacccttca ggaagcaatt tcgcaaaagc ttctgctact tttttgttgt
    10081 aagaatcttt tcttaaacta ccaataaaaa atccaatttt ttttgtcata cgatttttcc
    10141 tccttcaaac tatctataat aatcttacaa aagataagta actggagcaa ataaaaacac
    10201 ttactgacaa aagctacata gaagatagat tacttatttt taacaaaaaa gaagtctaaa
    10261 atcttctatt ttctaaaaaa aatcgaattt aatcaagctg taaagaaaaa aagctttaca
    10321 gggtaaaaaa aaactgttac actacatctt gtgaataaaa caatggaggt ggaaataaat
    10381 aatggcagtt aaaatccgtt taaaacgtat gggttctaaa aagagtccgt tttaccgtat
    10441 cgtcgtagct gattcacgtt ctcctcgtga tggacgtttc atcgaaactg taggtactta
    10501 caatcctttg aaagaccctg cagaagtagt tttaaaagaa gacttagttc ttgactggtt
    10561 atcaaaaggt gcacaacctt ctgacactgt acgtaacatc ctttcaaaag aaggcgttat
    10621 gcaaaaacac cacgaagcta aattctcaaa gaaataaggt gaccggatat gaaagattta
    10681 agcgacttag tcttaacaat cgttcgtccg ctagtcactt atcctgatca agtcaagtta
    10741 gacgtcgtag agtcgaaaga cttttacgag tacaatctaa cagtggctcc tgaagatatt
    10801 ggccgtatca ttggaaaaca agggcgtgtt gctaaagcaa tccgcacaat cgtttacggt
    10861 gttcggatca atgcaccgaa aaaagtgcgt ttgaatatca tcgatgataa ggaaaagtaa
    10921 ataaacgtgc tgtactcgaa ttttcgagtg cagctttttt gtttttttta ttaattgaag
    10981 aaatgattat ttggcaaaac aagcaagaaa gcgtatcctt ataggaggag ggagtgaaca
    11041 gccaatgaga aagaaaatgt tatttattcc atttgctcta ttgttaagcg cttgtagttc
    11101 ggttactcaa gaatcagaca atacacatct atcaagcagt atatctgaaa ctgttaccag
    11161 tccagaaaaa agtagtacca aaacggcttc caccactgaa caaacaatgg ctacctcaaa
    11221 taatgagaaa aagactgctt tagaccagtt gaaagaacaa cagccaaatg ttcccatgcc
    11281 tctagatgta ccagtctctt caggctatct caatatcgca gcgacacata caaagcaagg
    11341 ttattctatt ttatattaca gaacagatcg tcccctcggg ttgaatgctg acgaattgaa
    11401 tcaagaaacg cctattgcaa cctatctata tcaatatggg tttgcttcta gtcaagaaac
    11461 aatccaagtt ttgcagccat ttgagatcga cacaaatggt cagcaagtgg acttaggttc
    11521 tcgaatcaca gggtatcagc aaggtgcagc aggttcaagt tttcttgaat ggcaagaagg
    11581 aaactggcgt attcgaatcc gtgggaataa tatagaaggc caagatccct tactattagc
    11641 taaagaaatc gtagcttatt tggaagaaaa cagtctacca gcacctgaac aatttggtaa
    11701 aattactgtt gatatgggtg atacgacgaa tcgagccgta gaagtaagct ggcaagaacc
    11761 gaaaaatgcc tatacgatca ctcatcaaga tcctatgtct gcgttgaaga tggcagtttc
    11821 tatgaagagg ttgtgaaatc agcgcgctga tctgagccca tcatttaact ccaagtcaca
    11881 ggaagtaatc aaaaatagca cgccgcattt ttgcataatt ttggcttaat accttaaaag
    11941 aaaactagaa ttgcctcttc acacatgcta aaataaaaca gacaaaaaag aaatggggat
    12001 ataagttgac tgaatacttg aatgtaggaa aaatcgtaaa cacacaaggt atcaaaggcg
    12061 aagtccgtgt tatctcaaca acggatttcc cagaagaacg ttacaaaaaa ggaacggtgc
    12121 taacactatt ccaagacgga aaagcacctg tggaattaac agtcaaaagt catcgcaaac
    12181 ataaaaattt tgacttattg agttttgaag gacatccttc tatcaatgat gtggaaaaat
    12241 atcgggatgg catattaaaa gtgtcaaaag acaactctgt agatctagct gaaaatgaat
    12301 tctattacca tgagatcatc ggtctgcatg tattagaaga tggaactgag ttaggtaaag
    12361 taaaagaaat cttgtcacca ggtgccaatg atgtttgggt cgttcaacgt ccgaaaaaag
    12421 ctgatatatt gcttccatat atcgcttctg tcgtcaaaga agtcgattta gaagctggag
    12481 tggtccgtgt agaaattcca gaaggactga ttgacgatga agattgatgt attgacttta
    12541 tttccaagaa tgtttgaagg tcctatgggg gaatccatca tcggtaaggc tgtagataaa
    12601 ggattattag atatcaatat ctctaacttc cgcgattatt cagataacaa acatcagaca
    12661 gtagatgatt atccatacgg cggtggagca ggtatgctgc tcaaagtcca gcccatctat
    12721 gataacatcc aagccattga aaaagctgca ccggatgtca aaaaaagagt aattcttctt
    12781 gacccagctg gtaaacgatt cgatcagaaa atggcagaag aattttcaca agaagaacac
    12841 ttagtattca tttgtggtca ttatgaggga tatgatgaac gtatccgcac attagtgaca
    12901 gatgaagttt ccttaggaga ttatgtcctt actgggggag aattaggtgc gatggtcatg
    12961 atcgatgcaa cagttcggct tttgccagat gtgttaggga atcagacttc agctcaaacg
    13021 gattcttatt caaccggttt attggaacat ccccaatata ctcgcccggc agaatataaa
    13081 gggatgaaag taccagaagt attgacaaat ggaaatcata aattgattga agaatggcaa
    13141 ttaaaagaat cactaagaag gacctatcaa cgtcggcctg atcttttgga aacacttgaa
    13201 tggacacctc aaatgacgaa atttttagaa gaaatcaaga aagaagaaca agaaaaaaca
    13261 gcagatgaag ccaagtgact ttttctaaaa tcttctttac agtaggttga taatatgtta
    13321 gtattttatg gtgagtgcaa aagcacttaa ctattacgat attccgctgt gagaaaagct
    13381 cataagaata tttggaataa ggagaagaaa accatgaatc cattaatcga agaattaaca
    13441 aaagaacaat tacgttctga catcccagcg ttccgccctg gtgacactgt gcgtgttcac
    13501 gcaaaagttg ttgaaggtac tcgtgaacgt atccagttat ttgaaggtgt tgttatcaaa
    13561 cgccgcggtg ctggaatcag cgaaacttat acagtacgta aagtttctaa cggagtgggc
    13621 gttgaacgta cattcccatt gcatacacca cgtgttgcaa aaatcgaagt agttcgctac
    13681 ggtaaagtac gtcgtgcaaa attgtactac ctacgtgcat tacacggaaa agctgctcgt
    13741 atcaaagaaa tccgtcgtta atcatcgtta tcagactgag aaagaaccct tggtattcaa
    13801 gggttctttt tttgttctct gtttttttat attaacgatt tctatttggg acatttttga
    13861 ttaataaaaa ggggcaaaaa aggggcaaca agttacagtt tttctatatc ttctttcaat
    13921 ttttctttta ctccctttgt cacatgcaaa taaatctttt cagtgatatc actattttcg
    13981 tgcccaacgc gatcctgaat agcatacaga ggagttccta gttccgctaa ttttgaaata
    14041 tgggtatgtc taaaaatatg tgaactaagt tttttatcaa tcttcatgtc agctttatga
    14101 tttctcaaat aggtgttaat tgctgttagt tggaaaggag ttcctttcgt tgtttgaaat
    14161 agaaattgac cattcggatt taattctaga agctcattgt aaatagctat cgcttttttt
    14221 ggtaaatcaa tttctcgcat tccagcagca gtttttgtag aatcagattt tttcatatca
    14281 gctattgaac gttctctata catcatcgtc ccatttataa ctactgatgc attattattg
    14341 gtaatgtgta catcgtcttt acttaatgcg attgcttctc caggcctcat tccagtcaaa
    14401 tatagccact ggaaaaggag agaatatctt ttattgtgtg aagttgtgaa atttactagt
    14461 cttttatatt catcatcttc taaaaactta tctttaattt ttatcgtttt tgactctcgt
    14521 ttataatcaa taactacttc gttgatagga tttttttcta catagccttt tttcatagcg
    14581 tatgaaaaaa gaagattcaa tttggattta attacactga cgtacttatt tgacaagtca
    14641 tctttatata tcatatcttc aaaaatatta tttaaatcta ctgtattaat accagaaacg
    14701 atgtaagtct caggtatctt tttctttata gtgtttaaga tattattcgt aggataatat
    14761 gtagactcct taacttgtct tttgtaaata actaaccatt cttcgacaag ctcatgaaat
    14821 gttagatcag gcttctgaag tgttttttcg tttaatttta tatcaatttt tttatttaac
    14881 tctagcaatg ccactttctg agtttctcga gatttatttt tataagtaat gctcactttt
    14941 tttctttttc tagtttttgg atcaatatat cgctcattat atttatatac tttttttccg
    15001 tttttatcag ttttggtttc aatccacatt gttttcatct cctatttgtt gctataatag
    15061 gaatggataa gtaagccaat tatagcaggt ttatttttca tcacgtccac aaactttggc
    15121 gagggagggg gcgtgttttt ttgtttagta gctacctttc catgaagtat ctgcgaagaa
    15181 cacttcaaat gttgttccat catttttcac ttcaaaatat gctgttcctt gtgcggattt
    15241 acccggttgg atggtttccg aaaagtaatc tctactagct aaatcagatt taatatctga
    15301 tgaatcataa aattctaaat aacttgaact tgcatcaaaa ggtgtagtcc ctgtgttttc
    15361 tacaagaaaa tctacctttg cgtaaaatag gccttctggt ttatagaaat catcaccgta
    15421 atcttttgat acagactgga cagttacgtt aatagagtta ccattttcag aagtaaaagg
    15481 aactgcttct cctatactat gtgcaactga tgccgtctgg ggttctgaag acgaagttgt
    15541 agttgtagtt gtagtagagc ttgaatcttt ggattttcca tctttatcta agaaagttcg
    15601 ttgattataa tatacaacct gttcagtttt tggactaacc caaattaaca ttgtttcatc
    15661 atcagaatca gttgaataag tatattggag catctctaat ttttttatat cttttgcact
    15721 atttccatgt tcaattaatt cagagtgctc tttataataa tctccttgtg catcctttga
    15781 aatagataca attaattcat cccgtgtgat agcattgtct aattctttgt aaacaaaatc
    15841 atttgtccat ttacgtaacg gtgaaccaag tgtctgataa acttgagtta aatcatcacc
    15901 aacttttatt ttatgaacgt ctgatgaagt tagttttttt gtttcatatt cagatgttga
    15961 tgaaacagta ttttctttag tgctagcaga attaggacta gtggagcatc cgcttaatac
    16021 caagcctaaa aaaagcaacc caaaaacaat ttttttcatt tttaattcct cacttctatg
    16081 atatgatttt tttgtaggat cttagaaata aggtttttag tccgtgttgc agcacggact
    16141 ttttttacat tatataagaa agcgataagc gctttctgga agtccgtaaa gattctttaa
    16201 ttcctcaatt cgtttaggat attgatcatt gtcttctttg tatagagaaa caatgagatt
    16261 agcagcaaag caattagctt cgctttcaga tttgcttcta gatgttcttg ttgatacata
    16321 gtaactggat aagccacgat gaaaaatagc gtgacctaat tcgtgagcgc aaatgtagaa
    16381 tctttcctca gagtctctca gttcatcatt taagaagatt attgcacgac ctctaatttc
    16441 ttgaaactgt cctttgggat tctcgataaa aggaacgtat tgaattttaa tgcccatctt
    16501 ttcacaaata taaaaaggat tagcggactg gtatttccgc ttcaactcct cgactaaatt
    16561 aatcgtatcc atctccataa gctcacatct ttttgccttt ttctttgtct tctttcacaa
    16621 tatcccagaa ggttccaata aggatatctt ttacgcgttg gacctgttca ggcgtcagtg
    16681 tttctccgcc ataagacata ttgacatttg aatctagtaa tttatcaagt tcaactactt
    16741 catcttttgt tgcccagtca ggaacttggt ttctaccgag taaataatca gtagttacat
    16801 taaaataatc tgctacaagt tgtagtcttt tagtactagg agttctattt ttccattgat
    16861 aaatcgtgtt tttaggtata tttaattctt cttccaattg agttatactt atgcctcttt
    16921 tatgagctaa ttcctttatt ctatctagta aattcatttt cgcacctcac aagctacgaa
    16981 aacactaata aaaaaattag ttaaaggtgt tgacaactaa taaaagtgtt agtatactgt
    17041 tttcgtaagc taaaatatta gctaaaaaga
//
LOCUS       DACDKM010000054        17019 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20834_2016
            SAMN12640370-rid9891663.denovo.054, whole genome shotgun sequence.
ACCESSION   DACDKM010000054 DACDKM010000000
VERSION     DACDKM010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640370
            Sequence Read Archive: SRR10040891
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17019)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17019)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742835.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742835.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:04:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,012
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 29 of 75
            Pseudo Genes (incomplete)         :: 33 of 75
            Pseudo Genes (internal stop)      :: 19 of 75
            Pseudo Genes (multiple problems)  :: 5 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17019
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640370-rid9891663.denovo.054"
                     /isolate="E20834_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..652
                     /locus_tag="IV334_001646"
     CDS             <1..652
                     /locus_tag="IV334_001646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002419316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS30-like element IS6770 family transposase"
                     /protein_id="HAP8943717.1"
                     /translation="GMRTLYRLFSKGIFDIDTLPMKGKRKPNGHQEKRGKQQYQRSIH
                     DRPDNYPDFNSEFGHLEGDTIVGIHHKSAVITLVERLSKVIITIKPNGRKALDIETAL
                     NQWFSRFPKNFFKSITFDCGKEFSNWKAISNQHDIDIYFADPGTPSQRPLNENSNGIL
                     RRNGLPKSMDFRKVNQTFISSVSNQRNHIPRKSLNYRTPIEIFLSYVQEAFYSSLI"
     gene            complement(723..2033)
                     /locus_tag="IV334_001647"
     CDS             complement(723..2033)
                     /locus_tag="IV334_001647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 28 protein"
                     /protein_id="HAP8943718.1"
                     /translation="MRTKKGIINALNFKTNDTQQDDTIILQKAIDQGAKEHLPVFIPK
                     GIYLVGALFLKDKSHLIFEEGAVLKGRTDIEAFPEIDTRVAGVEMKWPAAILNVLSAK
                     DILIEGKGIIDGQGDHWWELYWGKDQKSGTRAEYDQKGLRWIADYAIKRPRACLLYHA
                     EHVVVRDLIFQKSGFWNLQITYSNDVLVEKVIIRNNDGPSTDGIDIDSSTNVRVYECD
                     LACGDDCIAIKSGRDGNGARVNRKSSRIEVARCKIRSGYGVTIGSEVSAGVSDVYIHD
                     INFFQSDCGIRMKSSKERGGVIENIRVENLNMIDVQFPFSWIMDWHNEYNRKNSNDLA
                     QMPESWQAVAEEIPESKQMTKVKDIHIKNVQACLSPGYSLPSRAFDLVAFPEKPIEDV
                     CFSHCQITAKEFGRIEAVQNLCFKSCDLSIETGNTAANNTFDNR"
     gene            complement(2045..3463)
                     /locus_tag="IV334_001648"
     CDS             complement(2045..3463)
                     /locus_tag="IV334_001648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP8943719.1"
                     /translation="MLSQNAVTANTVKKEKKEFGISDKIGYMFGDLGNCFILGLVNSF
                     LMIYYTNVLGVSGAIVGTLFLISRILDAFADVMVGRLCDVSELTETGRFIPWIRKMKY
                     PFCLVTVILFLPFVQHLPMTVKIAYIFVSYIVYGIFLSTINIPYGAMAAAISSNPNDR
                     TSLSTFRSIGSAIGGSTTGFFIPILMYTHNKSGQQVVSGNHFFWISIGCAAIAFVAYM
                     LTCKLTTERVRVVKNEKVSASQLVKGLLANRALIILVIVDIFIVINQILAGTNMTYLF
                     NDYFHNKQAMSVALLFTYGTVVVLAPFATKLTKLFGKKEASVFALLASSCMYLLMYFL
                     HITNAWVYLILLFIATLGSGLFNLMVWAFITDVIDYHQYKTGLREDGTVYGVNSFARK
                     VGQACAGAVGGFMLALIGYQSSTTGGAIQSVLVQERIYTMANLLPAFCLFVSAIILLV
                     GYPLNRSKTVFIGEELKKINER"
     gene            complement(3703..5271)
                     /locus_tag="IV334_001649"
     CDS             complement(3703..5271)
                     /locus_tag="IV334_001649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 43 protein"
                     /protein_id="HAP8943720.1"
                     /translation="MQTIKVKNPILRGFNPDPNILRVKERYYIIVSSFEWLPGIRVYE
                     SKNLVNWSHTTDILTTQVSLQGNPTNGSIWAPQISYADGLFYLVYTDVKSAGRPFKDV
                     HNYLITASEITGPWSEPIYLNSNGFDPSLFHDLETGKKWLVNELWDYRLDTPNKSSGI
                     VLQEYDAEKKKLDGPIYKIFAGTELAKTEAPHLYFRNGYYYLMTAEGGTGRGHSVTVC
                     RAKNITGPYELDPGFPMLTASDKPASTLQCTGHGSLVQTPSGRWYMTYLCTRPLNGAA
                     ILGRETAIQEVYWTSDDWLRLKDSQTTPMEEIMIETNEEVQQEKDHQFMDTFEGELKK
                     NWNARRILPNADWCDTKCRPGFLRLISGESLQSTFEHHLLAIRQTDFTFDAETAFEYM
                     PNNFNQMAGLVLYLNESNYIYCYVTWNEDKGKCLRMIESENGVAQIEDWTIPLTEKKT
                     FLKVTVKKETAQFHLKDVSTEEWKKVGPQKNMCILSGGFTGNFIGIAVHDLNKKRGSY
                     ADFEFFKYDGKDHL"
     gene            complement(5275..5556)
                     /locus_tag="IV334_001650"
     CDS             complement(5275..5556)
                     /locus_tag="IV334_001650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4064 domain-containing protein"
                     /protein_id="HAP8943721.1"
                     /translation="MKRKWELLLGMVGGSLSLIFFGGLAVTLSNMSASEFKKSYQSLA
                     VDHSTLSLENTFGLLQDMTGLFAVVLFISLAFLAAGAFSLYRVRMRKGA"
     gene            complement(5553..6476)
                     /locus_tag="IV334_001651"
     CDS             complement(5553..6476)
                     /locus_tag="IV334_001651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pectin esterase"
                     /protein_id="HAP8943722.1"
                     /translation="MRMKDKLIIGKNAIDTPTDFETIQEGIDYLAGLPDSHPKVLIVK
                     EGRYDEYIESRLSNFKLVGAGTVVISGNRFAKQEYLDGSQLGTFRSATFFLEGSNVVL
                     ENLHIENTAGPGEKVGQAVALFNSGDRVSLKNCRLSGYQDTLCTGPLPDLQKDGTPFC
                     TPASANPEYCRQTYEYCWIEGTVDFIFGGADAVFHQCHIHSLASKNPGYITAASTPKN
                     QKTGYRFESCTLTAEPDTRNVYLGRPWRPYANVLFEACKIGGHVHPTGWDNWDDKKNE
                     QTVKFSERNNNYETSIKRPEWIHLEKQGVQK"
     gene            complement(6473..7351)
                     /locus_tag="IV334_001652"
     CDS             complement(6473..7351)
                     /locus_tag="IV334_001652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HAP8943723.1"
                     /translation="MQLNDFLGAPSSRHTPSGKRLYREKQNGFMLEYYQLYLNELETV
                     PALYAYPEKEGPFPTVLYLHSHGGDFSVGKSELIHGAPYLASPSFAEVLTGMGYAVWS
                     IDAWGFEERGGISESELFKQFLLTGKTLWGMRIYDVISLIDYLETREDTDTQRLATIG
                     LSMGGLLSWWVTALDSRVKVCIDLAAQVDIETLLLHRRLDHHGFYYYVPNLLTAYTTL
                     AIQEKIAPRPRLSLVGKNDTMCLDEGVLKLRKGLDKKYQSMGSPENFSSFSLTGGHME
                     TQEMRNIWKQFIREHL"
     gene            complement(7338..8675)
                     /locus_tag="IV334_001653"
     CDS             complement(7338..8675)
                     /locus_tag="IV334_001653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 28 protein"
                     /protein_id="HAP8943724.1"
                     /translation="MYDILTFGASIDELNTEAIQQAIDAAASDGGGTVVVPAGEFLTG
                     ALFLKSNVELHLSAGAVLKFSDDPKDYPVVHSRWEGVHRKVYASCIYAQNVENISVTG
                     FGTLDGNGKKWWHTFRNEPDNLAYPRPKLMSFHNCHRITVKDIKLIQSPSWTINPILC
                     SNATFDNLTILNPADSPNTDGIDPESCKNVRISNCHIDVGDDCIAIKAGTEDTYERIA
                     CENITITNCTMVHGHGGVVLGSEMSGSIRNITISNCIFQETDRGIRLKSRRGRGGIVE
                     DIRVSNIVMDNVMCPFILNLYYFCGPRGKEPYVWEKKAYPIDERTSAFRRIHFSNITA
                     RNVHASAGFIYGLAEQFIQEITFDNIDVSMAQDAVPGVPAMMTGIEEMSRQGFYIGFA
                     GKVSFNQMSIENHEGPAFLLENTKEISIEKFQSNQSENMDELVVRKNFAYAVE"
     gene            complement(8678..9781)
                     /locus_tag="IV334_001654"
     CDS             complement(8678..9781)
                     /locus_tag="IV334_001654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 88"
                     /protein_id="HAP8943725.1"
                     /translation="MENWSKKLAKSIMERTPRLYEEKWYKGKWSYDYGVVLKGFQLLW
                     ERTQEKIYFDFIKDNIDYFVQEDGTIRGYSVEEYNIDHVNTGKLFFLLYKETGEEKYK
                     KAAELLSQQLANHPRTSEGAFWHKEIYPYQIWLDGLYMGSPFYAEFIQNFSEEKDYQD
                     VLRQFEISYKHLFDPKTNLLIHAWDEKKVQPWADPKTGLSHHFWSRSIGWYLMAAADT
                     YELLHEETDCSLLKEILEKTLAALLPYQEENSGTWYQVTTETKRKGNYLEASGSSMFV
                     YAMAKGIRLGLLNKEEWLPQVKKSHQGLLDEFVLETKEGWLNLNKNCQVAGLGGADQR
                     DGSYAYYISEPIICNDQKGVGAFLQALIEVEAL"
     gene            complement(9855..11363)
                     /locus_tag="IV334_001655"
     CDS             complement(9855..11363)
                     /locus_tag="IV334_001655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HAP8943726.1"
                     /translation="MKKATLVKLGLSFAGILALAACGSSSSNSSSETEDGKAKITWLN
                     ILHHASPPTDTIVNLIEEKTDTELTFNWIPDASKEERLTTALASDELGDIVTLQIAML
                     KNSSVRNALKSGVFWDVSEYLKDYKNLSQISEDRIEAASIDGALYGVPIQKDYARAGL
                     VIRKDWLDNLGLDVPTTMDELYEVAKQFTENDPDGNGVDDTVGFGDRAPSDIRYTSFK
                     LFTSYFGAPNGWAVDDNGKFTPEFDTEEYMKALNFSRDLFENGYLAQDFAVTQKTDQQ
                     EQFAQGRTGIYTGMVDIKNLKTMGQGIQDDMELVPVNKISDGEGGGYHVWSEGNGIGG
                     LLAFPKSQVKDEAQLKKLLQFVDDLLEEDVYKLMTGGIEGTHYELNDDGSIEFIDMDL
                     WQQEVQPLSSSRPSEVTFSFKDTDPEKEFSNQLIKENEEFAVMDPSVPLDSPTNNEVG
                     SEIEKIVIDATVQYIMGQLDEDGFKKAVEDWKAQGGDRITQEYEEAYKAAAQ"
     gene            complement(11329..12021)
                     /locus_tag="IV334_001656"
     CDS             complement(11329..12021)
                     /locus_tag="IV334_001656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HAP8943727.1"
                     /translation="MLQSLDKINGRLNRILQLVYLNFLWGGLVIAGLGVFTFGPASYA
                     MVAVIRKWLRSKETFPLTKTYFTYFKENYRETLLMSWIYGAIGIVLLVDLSFIHNWYL
                     RIALYIAAFIYVLSALYIFPIMAHYDWKGYFFKMKMAFLFGFSHLHYSLVLILSMVGI
                     YLMIIQTMPGVLTFIGGSLFFSAVTWTANSIFCHLEWKNSYTEKTEENKNIIKTVKEK
                     IYEKSNARKIRS"
     gene            complement(12043..13017)
                     /locus_tag="IV334_001657"
     CDS             complement(12043..13017)
                     /locus_tag="IV334_001657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP8943728.1"
                     /translation="MESVKKQEPFQLTLSKKEEKLRRRKKIIAGIKTNKFLYVMILPG
                     VLYFLIFRYLPMGGLVIAFQDYQPFLGIMGSPWVGFKHFIRLFTEPTFFTLLRNTLIL
                     FGLNIFVFFPMPIILSLLLNEVTNKRLKNGIQTIIYIPHFMSWVIIVSITYVFLNVDG
                     GVLNEFLATMGFEKISFLTSPEWTRTVYIGQVIWKELGWSTIIYLSAITAVDTQLYEA
                     AEMDGAGRLRKTWHVTLPAIRPVIITLLILKIGQTLDLGFEHMYLLLNSLNRSVAEIF
                     DTYIYTAGLKNGQLSFSTAMGLFKGVVGLFMVILANKIAKKLGEDGVY"
     gene            13317..14189
                     /locus_tag="IV334_001658"
     CDS             13317..14189
                     /locus_tag="IV334_001658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAP8943729.1"
                     /translation="MKKKVSIGERSFTLFNNTFLILLALICIVPFLNIIATSFASTQE
                     VVAKKFILFPTTFSLDAYRYILSTPTIFRALAVSIGVTGVGTIVSMCATSLMAYGLSR
                     KYLFGRGFVNFLVVFSMLFSGGMIPTFLVVRSLGLVNSYWAMILPVAVNAMNMIIMRN
                     FFQALPDSLEESAKMDGCTDFGVFFKIMLPLALPSIATISLFYAVTYWNTYMTAILYI
                     NDSSKWPIQILLRQIVIVSSGMQAESSAVDVIPPAQTVKMAVIVIATVPMLIVYPFVQ
                     KYFVKGALVGSVKG"
     gene            complement(14771..16969)
                     /locus_tag="IV334_001659"
     CDS             complement(14771..16969)
                     /locus_tag="IV334_001659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP8943730.1"
                     /translation="MKKSTVFYKIFLPMFLLGAVLVIGFSLFIYQNTYESIESSYLTD
                     KKNLLRQVKTNVEWKIRTIEYSFSTYGSTKNFSDIFKHPLTYTDYSTYSEIRKELNFI
                     ETIVMDDNNYDLVSLAGKWGVINGSLSQLTEEEVEKYQQKYINNKNNLFWQKKNKGIE
                     MVISLPMFEKEKYALGIATIEKHTIEQVVGESGEDILSIENEVGNLFTTQTVKNKEQP
                     TVLTDVDLDYNQLKVIREKDKTYIVLKSDYNNWIYRLEVDSSAIGAMIRNLRIGLVAV
                     SLTLVGLIGLFSYLFSDRFARPISQIQERLNLKSKGFSGKELDLVAQSVSRIIGEKEA
                     LSAHLVTQKPQLETLFVLSLFRNRVERRELDQRLQQFGYSSQNDCYYTALVQIDLLED
                     SHGGERDLLLLAINNMITEIVPKENRMLPIVLNEEMQATIYRMDKDDAEASKKVMEYC
                     RLIQKRVKEYLNLTISIGISNRFETLNESKQSVDRAKEALYYRVNTGPSSIIFYQEIV
                     PAAHEKTLIRYPIEEQNRLFEAIRSGNQEVKNLVHELIDALFAQNKNPLSREVVSVRL
                     INELVQLGQLLGVDSKNFDNMKQIYVKALNNYHPKELEKLVLEQLVLPITECSQAITD
                     HEFKNLSENIMHIVYTEYDRDLSLDVIADRLHYNPNYLSNVFKKETGEKFGDFVQNHR
                     LEVAKKWLKETNLSVKEIAERLQYRNPQNFIRFFKKKESITPGEYRKKYL"
BASE COUNT         5036 a         3568 c         2770 g         5645 t
ORIGIN      
        1 cggtatgaga acactttatc gtttattttc taaaggaata tttgatattg acacactacc
       61 gatgaaaggt aaaagaaaac ccaatggcca tcaggaaaaa cggggaaaac aacagtatca
      121 gcgctcaatc catgatcgac ctgataatta tcctgatttc aattctgagt ttggtcacct
      181 tgaaggtgat acgatcgttg gcattcatca taaaagtgcc gtcattactt tagttgaaag
      241 attatctaaa gtcattatca cgattaaacc caacggccgt aaggcattag atattgaaac
      301 tgcccttaat caatggtttt ctcgcttccc taaaaacttc tttaaatcta ttacgtttga
      361 ctgtggaaaa gaattttcta actggaaagc cattagtaac caacatgata ttgatatata
      421 ttttgcggac cctggaacac cttctcaacg cccattaaac gagaattcta acgggattct
      481 gcgtcgtaat ggactgccga aatcaatgga ttttagaaaa gtgaatcaga catttatttc
      541 cagtgtcagc aatcaacgta atcatattcc aagaaaatca ttgaattaca gaacaccaat
      601 cgaaatattt ttgagctatg tacaagaagc attttattct agcttaattt gacaaatcat
      661 atatcattaa aaaaaataaa ggtcaagcta aaatattatt taagcttgac cttattataa
      721 tttcaacgat tgtcaaaagt attgtttgca gctgtgttgc cagtttctat agataaatca
      781 cagcttttga aacataagtt ttgtactgct tcgattcgcc cgaattcttt tgcagttatt
      841 tggcaatgag aaaagcagac atcttctata ggcttttcag gaaatgctac taaatcaaaa
      901 gctcgggaag gaagtgagta acctggagaa aggcatgcct gtacgttttt gatgtgtatg
      961 tctttgactt tcgtcatttg ctttgattca ggtatttctt cagccactgc ttgccaactt
     1021 tctggcattt gagcaaggtc attgctattt tttctattgt attcattatg ccagtccatt
     1081 atccaagaaa aaggaaattg tacatcaatc atattcagat tttctaccct gatattttcg
     1141 atcactcccc ctctttcttt tgaagatttc attcgaatac cacagtctga ctgaaaaaaa
     1201 ttgatatcgt gaatatatac gtcactaact ccagcactaa cttcgctacc aatagttaca
     1261 ccgtatcctg aacggatttt acatctagca acctcgattc ttgatgattt tcgattgact
     1321 cttgcaccat tgccatctct gccagactta atagcaatac aatcatcacc acaggccaaa
     1381 tcacattcat agacacgcac atttgttgaa gaatcaatat caatcccatc tgtacttggt
     1441 ccatcattgt tgcggatgat tactttttct actaatacat cgttagaata agtgatttgc
     1501 aagttccaaa aacctgattt ctgaaaaatc aaatcacgaa caacaacatg ttctgcatga
     1561 tacagtaaac aagcgcgtgg acgtttaata gcataatccg caatccaacg aagtcccttt
     1621 tgatcgtatt cagctcttgt cccgcttttt tgatcttttc cccaatacag ctcccaccaa
     1681 tggtctcctt gaccgtcgat tattcctttt ccttctataa gaatatcttt cgccgagaga
     1741 acattcaaaa tcgctgctgg ccatttcatt tctacgccgg ctactcgtgt atcgatttct
     1801 ggaaatgctt cgatatctgt tctccccttg aggactgcac cttcttcaaa gataagatga
     1861 ctcttatctt ttaaaaaaag ggctccgact aaataaatgc cttttggaat aaatacaggt
     1921 aagtgttctt tcgcaccttg atcgattgct ttttgcagta ttattgtatc atcttgttga
     1981 gtgtcgtttg ttttaaagtt caaagcattg ataatccctt tttttgtccg caatacgttt
     2041 ctccctatct ttcattgatt tttttcaatt cttcaccaat aaatacagtt tttgaacgat
     2101 tcagtgggta acctactagc aaaatgattg cggatacaaa taagcagaat gctggtaaca
     2161 agtttgccat cgtataaatc ctttcttgta ctaaaactga ttggatggct ccacctgttg
     2221 tagacgattg gtaaccaatg agtgccagca taaatccgcc taccgctcct gcacatgctt
     2281 gtcctacttt tctcgcaaaa gaatttactc catatactgt tccatcttct cttaagcctg
     2341 tcttatattg atgataatca atcacgtcag taatgaatgc ccagaccatc aaattgaata
     2401 aaccagatcc taaagtagcg ataaataaaa gaatcagata tacccatgca tttgttatat
     2461 gaaggaaata catcagcaag tacatacatg aagaagcaag taacgcaaat acgcttgctt
     2521 cctttttacc aaataatttt gtcagctttg tagcaaatgg tgctaaaact actactgttc
     2581 cgtaagtaaa taaaagagca acagacattg cttgtttatt atggaagtaa tcattgaata
     2641 aataagtcat attcgtccca gctaaaatct gattgatcac aatgaaaatg tcaacaatga
     2701 ctaaaataat cagtgctcga ttagctaaca aaccttttac caactgagaa gcagatactt
     2761 tttcgttttt gacaactctt actctttcag tagtcaattt acaagttaac atatatgcga
     2821 caaatgctat tgcagcacat ccgattgaaa tccaaaagaa atgattccct gaaacgactt
     2881 gttgtccact cttgttatgg gtatacatta aaatcggaat gaagaatcct gtagttgatc
     2941 cgccaatcgc agaaccaatg cttcggaacg tggacaacga ggtgcggtca ttaggatttg
     3001 agctaattgc tgctgccatt gctccatacg ggatattgat tgttgagaga aaaatcccat
     3061 aaacaatgta tgaaacaaaa atataagcaa tcttcaccgt cattggaaga tgttggacga
     3121 atggcaaaaa caaaatgaca gtcactaagc aaaatggata cttcattttt ctgatccaag
     3181 gaataaatcg gccagtttct gttaactctg agacatcaca taatcttcca accatcacat
     3241 cagcaaacgc atccaaaatt ctcgaaatca agaataacgt acctacgatt gcccctgaaa
     3301 cgccaagaac attcgtataa taaatcatca agaaactatt gactaaacct aaaataaaac
     3361 agttacctag atcaccgaac atgtagccta tcttatcgga aatcccaaat tccttttttt
     3421 ctttttttac tgtatttgct gtaacagcat tttgtgaaag catttgaatt cctcctacct
     3481 cttctagtaa acgttttcaa aattataact ctttttttct tgtttttata ttcacaatgt
     3541 tggcttttta ttgcattatc ttgacagata aaaaacaaaa aataatctaa aaacattgat
     3601 gcataagatt gataactttt tttacataac aatatataca aacaaaaaaa ttcactaact
     3661 agtgaaaata agttagtgaa cctcatttat taaagaatta aattacagat gatcttttcc
     3721 atcatacttg aaaaactcaa aatcagcata acttcctctt tttttattta gatcatgcac
     3781 tgctatcccg ataaaatttc cagtaaaccc tcctgagagt atacacatgt ttttttgcgg
     3841 acctactttc ttccattctt cagttgatac gtctttcaaa tgaaactgtg ctgtctcttt
     3901 ctttacagtg actttcaaaa aagtcttctt ttctgtgagt ggaatcgtcc aatcttctat
     3961 ttgtgctact ccgttttctg attctatcat acgtaggcat tttcccttgt cctcattcca
     4021 agtaacataa caataaatat aattggattc atttaagtac agtaccagtc cagccatctg
     4081 attaaaatta ttaggcatgt attcaaatgc tgtctccgca tcaaaagtaa aatccgtttg
     4141 ccgaatagct aacaaatgat gttcgaaggt agattgaaga gattcaccgg aaatcagacg
     4201 taaaaaacct ggtctacatt ttgtatcaca ccaatcagca tttggcaaaa tgcgtctggc
     4261 attccagttc ttctttagct ctccttcaaa tgtatccata aactgatgat ctttttcttg
     4321 ttgtacctct tcatttgtct caatcattat ttcttccatc ggagttgtct ggctatcttt
     4381 aagtcttaac caatcgtcgg acgtccaata cacttcttga atagcggttt ctcttcccaa
     4441 aatagccgca ccgtttaatg gtctggtaca aagatatgtc atgtaccatc ttcctgacgg
     4501 ggtttgtact aagctcccat gtcctgtaca ttgcaatgta gaagcaggtt tgtcactagc
     4561 tgtcagcata ggaaaaccag gatcaagctc gtaaggacca gtaatattct ttgctcggca
     4621 aactgtaact gaatgtcctc ttcctgtacc gccttctgcc gtcatcaaat aataataccc
     4681 gttacgaaaa tacaaatgtg gtgcctccgt tttcgctaat tctgtacctg caaatatttt
     4741 atagatggga ccatcaagct tcttcttctc tgcatcatat tcttgtaata cgataccgct
     4801 ggatttattc ggtgtgtcca gacgataatc ccatagttca ttgacaagcc atttttttcc
     4861 cgtttcaaga tcatggaaca aagaaggatc aaagccgtta ctatttaaat aaattggttc
     4921 ggaccaagga cctgtgatct cagatgcagt gatcaaataa ttgtggacat ctttaaacgg
     4981 acggcctgca cttttgacat ctgtgtaaac aagataaaac aatccatcag cataactgat
     5041 ctgtggcgcc catatacttc catttgtagg atttccttgc aaagatactt gagtggtcaa
     5101 aatatccgtt gtatgtgacc agttgactaa atttttcgac tcatatactc ggattcctgg
     5161 aagccattca aaagaggaga caataatgta ataccgctct ttcactctta atatattagg
     5221 atctggattg aatcctctta aaataggatt tttcactttg atagtctgca tgattcaggc
     5281 tccttttctc attcttactc gatataaact gaatgcccca gctgctaaaa aagcaagtga
     5341 aataaacaac acaacggcaa acagaccagt catgtcctgt aataatccaa atgtattttc
     5401 taaagacaaa gttgaatgat ctacagcaag cgactgataa ctttttttaa actctgacgc
     5461 agacatatta cttaaagtaa ctgctaaccc gccaaagaaa atcaaagata gactgccacc
     5521 gaccattcct agcaatagtt cccattttct tttcatttct gaaccccctg cttttctaaa
     5581 tgtatccatt caggtctttt gatcgatgtt tcataattgt tattacgttc agagaatttg
     5641 acagtctgtt catttttctt gtcatcccaa ttatcccaac cagttgggtg aacatgccca
     5701 cctatcttac acgcttcaaa taagacattt gcatacggac gccacggtct tcctaaatag
     5761 acgtttctag tatcaggttc tgcagtcaag gtacagcttt caaaacgata ccctgttttt
     5821 tgatttttcg gcgtagatgc tgctgtgatg tatccaggat ttttactggc taagctatgg
     5881 atatgacact gatgaaaaac ggcatctgcc ccaccaaata tgaaatccac cgttccttca
     5941 atccagcaat attcataggt ctgacgacaa tactcaggat tggcagaagc cggtgtacaa
     6001 aaaggagtac catctttttg caaatcaggc aacggacccg tgcataaggt atcctgataa
     6061 ccagatagtc gacagttttt taaagatacg cgatctcctg aattgaataa agcaacagcc
     6121 tgtcctacct tttctcctgg acctgctgta ttttcgatgt gaagattttc taatacgaca
     6181 ttcgatcctt ctaagaaaaa agtagcactt cggaaagtcc ctaactggct accgtcaaga
     6241 tactcctgtt ttgcaaaacg attaccggag ataactactg ttcctgctcc cacaagttta
     6301 aaattggata atctgctttc tatatattca tcatatcttc cttcttttac tatcaagacc
     6361 tttggatggc tatccggcag gcctgccaaa taatcgattc cttcttggat agtttcaaaa
     6421 tccgtcgggg tatctatagc attctttcca attatcagtt tgtctttcat cctcataaat
     6481 gctcccttat gaattgtttc caaatatttc tcatttcttg tgtttccata tgaccgcctg
     6541 ttaaagaaaa agaagaaaag ttttctggac tacccattga ttgatacttt ttgtccagcc
     6601 cttttctcag cttcagtacg ccttcgtcca agcacatcgt atcgtttttt ccaactaagc
     6661 taagacgagg gcgcggagcg attttttctt ggattgctaa cgttgtataa gctgtcaata
     6721 gatttggcac atagtaataa aacccatgat gatctaggcg gcgatgtagc aaaagtgttt
     6781 cgatgtccac ctgtgccgct agatcaatac aaactttgac acgagaatcc aatgctgtca
     6841 cccaccagct taataagcct cccatagaaa gaccaatcgt tgcgagccgt tgagtatctg
     6901 tgtcttccct tgtctccaaa taatcgatca agctgatcac atcataaata cgcattcccc
     6961 ataacgtttt tcctgttaat aaaaattgtt tgaacaactc gctttcagaa atacctccgc
     7021 gttcttcaaa tccccaagca tcaatcgacc aaacagcata tcccattcct gtcagtactt
     7081 ctgcaaaaga tggagaagct aagtaaggag caccatggat caattcactt ttcccgactg
     7141 aaaaatctcc gccgtgagaa tgcaggtaaa ggaccgtcgg aaacggtcct tctttttctg
     7201 gataagcata gagggctggt actgtttcca attcatttaa gtacaattga tagtattcaa
     7261 gcataaaacc gttttgtttt tctcggtata atcgttttcc actaggggta tgtctgctag
     7321 atggagcacc taaaaaatca ttcaactgca taagcaaagt ttttacgaac aaccagttca
     7381 tccatatttt ctgattgatt tgattggaat ttctcaatac tgatttcttt agtattttct
     7441 aatagaaatg ctggtccctc gtgattttca atcgacatct ggttaaagga aaccttaccc
     7501 gcaaagccaa tataaaatcc ttgtctgctc atttcctcta ttcctgtcat catagcagga
     7561 acaccgggaa ctgcatcttg ggccatcgag acatcgatat tgtcaaatgt gatttcttgt
     7621 atgaattgtt ccgctaatcc ataaataaaa cctgcagaag catggacatt ccttgccgtg
     7681 atattcgaaa aatggatcct tctgaatgct gatgtccgtt catctatcgg ataagccttt
     7741 ttttcccata catacggttc tttccctcgc ggaccacaga aataataaag attcagtata
     7801 aatggacaca tcacattatc catgactata ttggatactc gaatatcttc aacgatcccg
     7861 cctcgacctc gtctagattt caatcgtatt cctcgatcag tttcttggaa gatacaattt
     7921 gaaatggtaa tattacgaat actaccactc atttcacttc ctaatacgac accgccatgt
     7981 ccatggacca tcgtgcaatt tgtgatcgtg atattttcgc aagcgatcct ttcgtacgta
     8041 tcttcagtcc ctgccttgat tgcaatacag tcatctccca cgtcaatatg gcaattacta
     8101 atacgcacat ttttgcatga ttctggatca attccgtctg tatttggtga atccgctggg
     8161 ttcaagatcg tcaagttatc aaacgttgca ttactgcaaa gaatcgggtt gatcgtccaa
     8221 cttggtgact ggatcaattt aatatctttc acagtaattc gatgacagtt atggaaactc
     8281 atcaatttag gacgaggata agcgagatta tctggttcat ttctaaaagt atgccaccat
     8341 ttttttccat ttccatctaa tgtaccgaaa ccagtgacag agatgttctc tacattttga
     8401 gcatagatac aggaagcata aacttttcga tggactcctt cccaacgtga atgaacaaca
     8461 ggataatctt ttggatcgtc agagaatttc agaaccgctc ctgcgcttaa atgaagttcg
     8521 acattgcttt ttaaaaataa agctcctgtt agaaattcac ctgctggaac aactacggtc
     8581 ccgcctccgt cacttgctgc agcatcaatg gcttgttgta ttgcttcagt attcaattca
     8641 tcaattgatg cgccaaatgt caaaatatca tacatattta taatgcctcc acttcaatca
     8701 atgcttgcaa gaatgcacca acgccttttt gatcattaca aataattggt tcactgatgt
     8761 agtaagcata tgaaccatct cgctgatctg ctccgcctaa ccctgctact tgacagtttt
     8821 tattcaagtt cagccagcct tcttttgttt ccagaacaaa ttcatcaagt aatccttgat
     8881 gtgatttctt cacttgtgga agccattctt ctttgttcag cagtcccaaa cgtattcctt
     8941 tagccattgc gtaaacaaac atactactgc cggaagcttc cagataattt cctttccgtt
     9001 ttgtttctgt tgttacttga taccatgtcc cgctgttttc ttcttgatat ggaagtaatg
     9061 cagccagtgt cttttctaga atttctttca gcaaagaaca atctgtttct tcatgaagta
     9121 attcataggt atctgctgca gccatcaaat accagccaat cgatcggctc caaaaatgat
     9181 gggatagacc tgtttttgga tcagcccatg gttggacctt cttttcatcc catgcatgaa
     9241 tcagcaaatt tgtttttgga tcgaatagat gtttataact gatttcaaac tgtctcaaaa
     9301 catcttggta atctttttct tcactaaaat tctggataaa ttcagcataa aaaggagaac
     9361 ccatatataa gccatccaac caaatttgat aaggataaat ttctttatgc caaaaagctc
     9421 cttcggatgt gcgtggatgg tttgccaact gctgggaaag gagttctgct gcttttttat
     9481 atttttcttc gcctgtttct ttgtacaata aaaagaaaag ctttcctgta tttacatggt
     9541 cgatattgta ttcttcaacg ctgtagccac ggatcgttcc atcttcttgc acgaaatagt
     9601 cgatattgtc ttttatgaaa tcaaaataaa ttttttcttg ggttcgttcc caaagtaatt
     9661 ggaacccttt aagtacgacg ccatagtcat aagaccattt tcctttatac cacttttctt
     9721 catataagcg aggtgttctt tccatgatac tttttgctaa tttctttgac cagttttcca
     9781 ttatttgctc tcctttaaaa attaaaatag gatttgggac aaataccaag aagtatttgt
     9841 cccaatcgtc tatattactg tgccgctgct ttgtatgctt cctcgtactc ttgtgtgatt
     9901 cgatcgcctc cttgtgcttt ccagtcttct acagcttttt tgaatccatc ttcgtcaagc
     9961 tgtcccatga tgtattgcac cgtagcatca atgacgattt tttcaatttc tgatcctact
    10021 tcattgttcg tcggtgaatc caatggcaca gaaggatcca ttacagcaaa ttcttcattt
    10081 tctttgatca attgattaga gaattctttt tcagggtctg tatctttgaa agagaacgtc
    10141 acttcactag gacgagagct tgataacggt tgcacctctt gttgccaaag atccatatcg
    10201 ataaattcga tagaaccatc atcattcaat tcataatgcg ttccttcgat accgccagtc
    10261 atcaatttat acacatcttc ttctaaaaga tcatcaacaa attgaagtaa tttcttcaat
    10321 tgtgcttcgt cttttacttg agatttggga aatgctagca agccgccaat accatttcct
    10381 tctgaccaaa catgataacc gccgccttca ccatctgaga tcttgtttac aggcactaat
    10441 tccatatcat cttgaatacc ttgacccatc gtcttcagat tcttgatatc caccattcca
    10501 gtgtagattc cagttcttcc ttgtgcaaat tgttcttgct gatcagtctt ttgggtaact
    10561 gcaaagtctt gtgccaaata gccattctca aatagatctc ttgaaaaatt caaagctttc
    10621 atatactctt ctgtatcaaa ttctggagtg aatttgccat tatcgtctac agcccagcca
    10681 tttggtgctc caaaataaga agtgaaaagt ttaaagctgg tataacgaat atcactaggc
    10741 gcacgatcgc caaaaccaac tgtgtcgtcc acaccatttc catcaggatc gttttctgta
    10801 aattgtttag ctacttcata aagctcatcc atggtagttg gaacatctag tcctagatta
    10861 tccaaccagt cttttcgaat gactaagcca gcacgagcgt agtctttttg tatcggtacg
    10921 ccgtacaagg caccgtcgat actcgcagct tcgattctat cttcagaaat ttgacttaaa
    10981 tttttatagt cttttaaata ttctgataca tcccagaata cgcctgattt taacgcattg
    11041 cggacagaag agtttttcaa catcgcaatt tgaagtgtaa cgatatctcc tagttcatca
    11101 gaagccaatg cagttgttaa tctttcttct tttgaagcat caggaatcca gttgaatgtc
    11161 agttccgtat cggttttttc ttcgatcaag ttcacaatcg tgtctgtagg cggtgaagca
    11221 tgatgcaaaa tatttaacca agtgatttta gcttttccat cttctgtctc tgaagaactg
    11281 ttacttgaac tgctgccgca tgcggcaagt gctagaattc ctgcaaaact aagacctaat
    11341 tttacgagcg ttgctttttt cataaatttt ctccttcact gttttaataa tatttttgtt
    11401 ttcttctgtt ttttctgtat atgagttctt ccattccaaa tggcaaaata tagaatttgc
    11461 tgtccacgtg actgcggaaa agaagagact gccaccgata aaggttaaaa cacctggcat
    11521 tgtttggatg atcatcagat aaatccctac catacttaat atcaacacta atgagtaatg
    11581 aagatgtgag aatccgaata aaaatgccat tttcattttg aaaaaatatc ctttccaatc
    11641 ataatgagcc atgattggga aaatatagag tgctgagagc acatatatga acgcagcaat
    11701 atacaaagcg atcctaagat accaattgtg tatgaaagaa agatccacaa gtaaaacaat
    11761 cccgatggcg ccatatatcc agctcatcag caaggtttca cgatagtttt ctttaaaata
    11821 tgtgaagtac gttttcgtca aaggaaacgt ttcttttgag cgcagccatt ttctaatcac
    11881 tgcaaccatc gcatacgatg caggaccgaa agtaaaaact cccaaacctg cgatgactaa
    11941 accgccccac agaaaattca aataaaccaa ctgtaaaata cgatttagcc ttccgtttat
    12001 tttatctaat gactgcagca tatctttccc tctctttcta tcttagtaaa cgccgtcttc
    12061 tcctagcttt ttggcaatct tattagctaa aattaccata aacaatccta cgaccccttt
    12121 aaacaatccc attgctgtac tgaaacttaa ctgtccattc ttcaatccag ctgtataaat
    12181 ataggtgtcg aaaatttctg caacgcttcg attcaatgaa ttcaataaca agtacatatg
    12241 ctcgaaccct aagtctaatg tctgaccgat ttttagaatc agcaaggtaa tgattactgg
    12301 acgaattgcc ggcaatgtaa catgccatgt tttgcgtaag cgtcctgctc catccatttc
    12361 tgcagcttca tacaactgtg tatcgactgc agttatcgct gaaaggtaga tgatcgtaga
    12421 ccagcccaac tctttccaga tcacttgtcc gatatatact gtgcgtgtcc attcagggga
    12481 tgttagaaaa ctgatttttt caaatcccat tgttgcaagg aattcattca atacccctcc
    12541 gtcaacattc aaaaatacat aagtaatcga aacaatgatt acccaagaca tgaagtgagg
    12601 gatgtaaatg atcgtttgaa tcccgttttt caatcgtttg ttcgttactt catttaatag
    12661 taaagacaaa atgattggca tcggaaaaaa cacgaagata tttaatccga acaagatcaa
    12721 cgtatttctt aataatgtaa agaatgtcgg ttctgtaaac aaacgaatga aatgtttgaa
    12781 accgacccaa ggacttccca ttattcctaa aaatggttga taatcttgaa aagcaataac
    12841 gagtcccccc ataggaagat acctaaaaat caagaaataa aggactcccg gcagaatcat
    12901 cacatataaa aatttattcg ttttgattcc tgcaattatt ttttttcgtc ttctgagctt
    12961 ttcttctttt ttcgatagcg tcagttgaaa tggttcttgt ttctttacac tttccataag
    13021 cccctcctta gttttgtgaa tgtttgctgc ttatgacttc atgatagata actatctgaa
    13081 ttacgtatat aatcattttt atcgtaagta aaaacgctta catagcaatg atttttgtgc
    13141 tatacaaaac gataaagtgt attttttcac aaataaacta cgcatagcaa accttctatt
    13201 tttttctatc tgacacttag cataggaaat gattatatac gtaatctttc accgatgata
    13261 aattgaacat atagaaacag aaacatttaa tttattttga ggaggagcct atctatatga
    13321 aaaagaaagt ctctatagga gaaagaagtt ttacactttt caacaatacg tttctaattt
    13381 tgctagcttt gatctgtatc gttccttttt taaatatcat cgctacatct tttgcttcta
    13441 cacaagaagt cgtcgcgaaa aagtttatcc tttttcctac taccttttct ttagacgcgt
    13501 atcgctacat tctttctacg ccgaccattt ttagagcact tgctgtttcg attggtgtta
    13561 caggtgtcgg aacgatcgtc agtatgtgtg caacttcact gatggcctac ggtctttcca
    13621 gaaagtattt gtttggtcga ggtttcgtca actttcttgt tgtattttct atgttgttca
    13681 gcggggggat gattccaacg ttcttagttg ttcgttctct agggctggtc aattcttact
    13741 gggccatgat cctgcctgtg gctgttaatg caatgaatat gatcattatg agaaactttt
    13801 tccaagcctt gccggatagt ttggaagaat cagcaaaaat ggatggttgc acagattttg
    13861 gtgtattttt caaaattatg ctgccattag ctttaccgtc tattgctact atctcgcttt
    13921 tttatgctgt tacttactgg aacacttata tgactgctat tttgtatatc aacgattcat
    13981 ctaaatggcc gatacagatt cttttacgcc aaatcgtcat tgtatcaagt gggatgcaag
    14041 cagaaagtag tgccgttgat gtgattccac cagcacaaac agttaaaatg gctgttatcg
    14101 ttattgcaac agtgcctatg ctgattgttt atccttttgt tcagaaatat tttgtcaaag
    14161 gagcactcgt aggttctgtc aaagggtgat tatgtactga acgcaaaatg gtagttgtgt
    14221 cagaagtggt cagcttcaag aaataagaag gcatttacga aaattgcttc ttaaattttt
    14281 gtgaaatttc ggcttatttt tcgaagaatc tactcctgga acaccgtgta tcagaggttg
    14341 tgaaagagac gttcagcctt gagcgatgat aaaaactctc acaaacattg cgacgagtaa
    14401 cccacaggag caataagaag cgattttgaa aaatagcttc ccatatttca caaaattatg
    14461 ccttattgtc gatagcctgt ctcttgaacg ccatttattc gtaaaaaaga actgtgacaa
    14521 gacttttgtc acagttcttt ttgcgtgctt agccttgaaa tttaactatc cataaataca
    14581 aatactaggg ttattctaac ttcttttatt gcttgaatca gtccacaaga aaaacagcct
    14641 cttgattaaa cggcgtttat cttgttgatt ctgcggcaat aaaatcagcc agcagcaaaa
    14701 catgaagtgc tgttttttgc tgtcctcttt tattgcttga atcagatcac aagaaaaaca
    14761 gcctcttgat tcacaaatat ttttttcgat attctcccgg cgtaatcgat tccttctttt
    14821 taaagaaacg aataaaattt tgcggatttc gatattgcaa tctttctgca atctctttta
    14881 cggagaggtt agtctctttc aaccattttt ttgctacctc taaacgatga ttttgaacaa
    14941 aatcaccaaa tttttctcct gtttcctttt taaatacatt acttaaatag ttagggttat
    15001 aatgcaacct atcagcgatc acatctaaag acagatcacg atcatattct gtgtagacga
    15061 tatgcataat attctccgac aaatttttaa attcatgatc agtgattgct tgagagcatt
    15121 ctgtgattgg cagaactaat tgttctaaaa caagtttttc tagttctttg ggatgataat
    15181 tgttcagtgc ttttacataa atttgtttca tgttatcaaa gttttttgaa tctactccca
    15241 ataactgtcc taattgaacg agttcattga tcaagcggac cgaaactact tcccgactca
    15301 aaggattttt attttgtgca aacaatgcat caatcaattc atgtactaaa ttcttcactt
    15361 cctgatttcc agaacgaatg gcttcaaaca atcgattttg ttcttctatc ggatatctaa
    15421 taagtgtttt ttcatgagca gcaggaacga tttcctgata aaaaataatt gaacttggac
    15481 cagtatttac acgataatac agggcttctt ttgcccgatc aaccgattgc ttgctttcat
    15541 tcagcgtttc gaaccggtta ctgatgccta tactgatagt taaattcaag tattctttta
    15601 cacgtttttg aatgagtcgg caatactcca tgactttttt gcttgcttct gcatcatctt
    15661 tatccattcg ataaatagtg gcttgcattt cttcattcaa aacgatcggc agcattcgat
    15721 tttcctttgg cacaatttcc gtgatcatgt tgttgatcgc taaaagtagc aaatcacgct
    15781 ccccaccgtg actatcttct aacagatcga tttgaaccaa agctgtataa tagcagtcat
    15841 tttgagaact ataaccaaat tgctgcaacc gctgatctag ttctcgcctt tctactcgat
    15901 ttctaaataa actaagtaca aatagcgttt ccagctgtgg cttttgggta actaagtgtg
    15961 cacttaacgc ttctttttct cctatgatcc gactgactga ttgagcgacc aaatctagtt
    16021 ctttcccact gaatcctttt gatttaagat tcagacgttc ctgtatttga gaaatcggac
    16081 gggcaaatct gtccgaaaat aagtaggaaa aaagaccaat gagtcctacc agtgttaaag
    16141 acactgcaac tagcccgatt ctcagattgc ggatcattgc gccaattgca ctagagtcaa
    16201 cttctaatct gtaaatccaa ttattataat ctgatttcag tactatatac gttttatctt
    16261 tttctcttat cactttcagc tgattgtaat ctagatccac gtccgttaat acagttggtt
    16321 gctctttatt ttttactgtc tgagtagtaa aaaggttgcc aacttcgttt tcaatagaaa
    16381 gtatgtcttc tccactttca ccaacaactt gttctattgt atgtttctct atcgtagcaa
    16441 ttcccaatgc atatttttct ttttcaaaca ttggcaaaga aatcaccatt tcgattcctt
    16501 tgtttttctt ttgccaaaat aaattatttt tgttatttat gtacttctgt tgatactttt
    16561 caacttcctc ttcggtcagt tgactcaacg atccattgat tactccccat ttaccagcca
    16621 aactgactaa atcataatta ttatcatcca taacaatcgt ttctataaaa tttagttctt
    16681 tcctgatttc agaataagtg gagtaatctg tatatgtcag cggatgcttg aatatgtccg
    16741 agaaattttt ggtcgaacca tacgtagaaa acgaatattc aatcgttcta attttccatt
    16801 caacgttagt tttaacttgc ctgagcaaat tctttttgtc tgttaaataa ctcgattcga
    16861 tcgattcata ggtattctgg taaatgaata aactaaatcc tatgacaagt acggctccta
    16921 ataaaaacat cggtaaaaat attttataaa atactgtact tttcttcatt ttcttcctct
    16981 cctcctttta aaataaaagg ctttcatttt tctgtaaaa
//
LOCUS       DACDKM010000055        17006 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20834_2016
            SAMN12640370-rid9891663.denovo.055, whole genome shotgun sequence.
ACCESSION   DACDKM010000055 DACDKM010000000
VERSION     DACDKM010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640370
            Sequence Read Archive: SRR10040891
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17006)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17006)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742835.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742835.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:04:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,012
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 29 of 75
            Pseudo Genes (incomplete)         :: 33 of 75
            Pseudo Genes (internal stop)      :: 19 of 75
            Pseudo Genes (multiple problems)  :: 5 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17006
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640370-rid9891663.denovo.055"
                     /isolate="E20834_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            30..404
                     /locus_tag="IV334_001660"
     CDS             30..404
                     /locus_tag="IV334_001660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8943731.1"
                     /translation="MEDKMYLRQKVFTKKDIENQINGFIQKLENANSYLINKELNKAY
                     DQWKEVYDELKLIQNETKLVRTEKKNENSFFFDGYAILMLETVAKQNIKAPKKELSDN
                     IDNALAELRYYVMQIKDVRITR"
     gene            634..978
                     /locus_tag="IV334_001661"
     CDS             634..978
                     /locus_tag="IV334_001661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HAP8943732.1"
                     /translation="MNEEFYRWLLQLIREDRLVKFYQSPKWRRLREKAMKRDHYECQE
                     CRRLGKYHRVENVHHIKEVKDRPDLALDLDNLICLCVEHHNEVHGRYLTALDKQEKKI
                     ESFANFDASERW"
     gene            983..1264
                     /locus_tag="IV334_001662"
     CDS             983..1264
                     /locus_tag="IV334_001662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8943733.1"
                     /translation="MIINDNGREYDTEKIEEYSSYTQGLIKRLIYVRYVGIRDLLSDN
                     CCSKYKVNQVREALNKDNNVERIKNVFGYSIEEINYYIDFAEAFIPMVR"
     gene            1367..1681
                     /locus_tag="IV334_001663"
     CDS             1367..1681
                     /locus_tag="IV334_001663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase"
                     /protein_id="HAP8943734.1"
                     /translation="MFKNELSQNRYREKLRRSLISQLESQKTNIEPFLDNVDRYISLW
                     ETAISLEEDISENGIRLENGKKNESVALLVSVNKQMGLMLDKLAITPELVGEANESIP
                     EL"
     gene            1659..3353
                     /locus_tag="IV334_001664"
     CDS             1659..3353
                     /locus_tag="IV334_001664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HAP8943735.1"
                     /translation="MNQFLSYKHIENWFKAIEEGTIKVCKEQLLLKNYLEERVFTRED
                     IYFDKQMVEDSINIPAQYFPFELIPWEKFLQCFIYGVRWKKDKTLVFNRYLSLMGRGN
                     GKTGFASWNNFFLLTAKHGIKNYDIDIYANNESQAKTSFDDVFKVIKDHPDLDKKVFK
                     ATKEVIQNIATNSKLRYNTANARTKDGKRPGANRFDEIHENEDYSMINVATSGGGKIR
                     DYREFYDTTNGHVRGGPLDDIIEESKMILSGELGIDKDGAEFSSLFPFICRLDNDNEV
                     DDPDMWEKACPTINYNANLKRKMFQEYSQMQRNAGLRLTFMTKRMNRPMEDTRFAVAS
                     YDDVLHTKEKEFPEKMDEVIGTVDFADRRDFASVGLLGKYDKDVYFTQHTFIHESALR
                     LQNIKREVIDISIDQGKSQIVHGKNIEADYIVGWFLEMSNKYYIKKIAMDMYRAKILK
                     PALEEAGFTVEIVRSGSVTHGMLKDLVDDLFINQRLFFGDDAIMRWYCMNVYEEHISN
                     GNIRYEKIEPETRKTDGFFSFLHGLNFLDDIYDSAPVTVTNSSVENTGTGFTPLVF"
     gene            3373..4551
                     /locus_tag="IV334_001665"
     CDS             3373..4551
                     /locus_tag="IV334_001665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HAP8943736.1"
                     /translation="MGIFQKAVGYFTKKATVPLEEYFCKLQVDFVYRKFAIETCIDLI
                     ANAMSKAEFKSYEDGKNKKNDLYYRLNVAPNKKNNATEFRKKLIRRLIFYNEVLIVSP
                     SNNSSEIFIADSWDVTEYALKDDVFSQVQINNIVLDREFLESDVIYIKYADQQIRQLV
                     DAYYQAYGKLISSAMNVYKRSNARRYVLKGNLFRPQDNTTQDQINKMMTSQFKAFMEA
                     DNAGAVFQLQNEYTLEDFSGNFQSNSRDIKNLIDDIFEMTAAAFHVPKNLLKGDMSGL
                     SDQVDAFLMFEIIPIAELIQDAFNASLYEVEEYLSGNFVRVDTTMIKITSFKDLVDAI
                     DVGIRNGVFTINEGRERVGNDRSDKAMADEIFITKNNQQVSKGGEANDDNENISSSKE
                     "
     gene            4514..5200
                     /locus_tag="IV334_001666"
     CDS             4514..5200
                     /locus_tag="IV334_001666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Clp protease ClpP"
                     /protein_id="HAP8943737.1"
                     /translation="MTTMKTFLAVKNEGAVPQIFIQGFIGSSWFFEGNTDKGIKNILD
                     SLGDQEEIEVVINSNGGDVFQGIAIGNLLKSNKAKVNVVINGLAASAASIIAMAGDTI
                     KIYNNAQLMIHRASTYGEGNVDDFRTIADQLESIDKSVKASYKTRFNGTDEALQELLE
                     KESFMDAETALSYGLVDEIIDAENSSGTEAKKEQSVEEILNDVKEKRAEKIAAFTAAL
                     NKTFGQGDVK"
     gene            5200..6360
                     /locus_tag="IV334_001667"
     CDS             5200..6360
                     /locus_tag="IV334_001667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HAP8943738.1"
                     /translation="MTVKNLKGVTAASDQLMKAFKDGNEESFSAAMVSLSKEIQDKIL
                     EEATAKNQDQLVLMNRGQRVLTTQETKFYNEVVKNEGFAGVEELVPATVFERVFEDLE
                     QSHPLLQKITFVNTTGVTEWIVSRGVNPAWWGKLCEAVKKVLDNGFDVINMKQFKLSG
                     YIPVCKAMLDLGPVWLDRYVRTVLVESLRIALEQAIVDGTGKDMPVGMMRDMSKQTSG
                     EYAEKKAEPITALDAVTMGRLMARLSKFNIEGVNDPIYRNVNPSDVVLIVNPTDYWSK
                     VFPAKTVLTANGEYVQVLPVPVSDLQSTAVPEGKAVIGVASDYFMGVGSTLKIEASDE
                     YHFVEDERIYLAKQYANGQPKRNDSFIVLDISALGTTTTTTKPTTTTTTTQA"
     gene            6370..7245
                     /locus_tag="IV334_001668"
     CDS             6370..7245
                     /locus_tag="IV334_001668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8943739.1"
                     /translation="MKYILCQPAINRFKWELEVCLTNLKKLGIKDIVLLFSRHDDQIP
                     IFFEKEYGVEVHVYDDLRDDKEYIPSIKPYLWWKYLEEDHSREDDRYFYIDSDVIFNK
                     RINLRKLPSKDDVWYCSDCCSYLSLDYIRSCENGENILKDMANIVNVTVESLETINTN
                     SGGAQWVINRPKANYWKKVYLDSNRLYRYLRGQKTNIQIWTAEMWARLWNMMYFNIGP
                     KVHEELDFCFATDPIEKVKEVKILHNAGVTTNDEDLFFKGRYVTSTPFDEDLSFVNKK
                     KCSYAYVKAIKAVVR"
     gene            7242..7553
                     /locus_tag="IV334_001669"
     CDS             7242..7553
                     /locus_tag="IV334_001669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8943740.1"
                     /translation="MTPEQVTEELLIAVKDNIYVTWNEEDESIKKMIAKNAVYLQSKV
                     STTLSFSPESLEYGLLIERCRYDWNRALDEFEQNFASELLGFIQHYALQEYIAGDVNG
                     E"
     gene            7543..7896
                     /locus_tag="IV334_001670"
     CDS             7543..7896
                     /locus_tag="IV334_001670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HAP8943741.1"
                     /translation="MANNRRLEETFNDGWLKILTQTTKRNELGKKIGVEDTEITSLKF
                     RNLSMRDSDITAMDAMGSKLTKKVKTPFHPIAKKFNKDQYFIVIDSMRYNVIYADYDN
                     FYIYFYLESVGEYGD"
     gene            7886..8287
                     /locus_tag="IV334_001671"
     CDS             7886..8287
                     /locus_tag="IV334_001671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8943742.1"
                     /translation="MVIDNSKEKERLNKQISAIKTSLEEHFGLKLFQDSVGEDELPDD
                     FNYFILETGEIEMITEPKYSVGQNLYLTFYSENREDLTGDSLDIISLIQNRSIRFQRM
                     DPNHLKLENQDRYIDQLVFTFRRLLKSDCHG"
     gene            8280..8684
                     /locus_tag="IV334_001672"
     CDS             8280..8684
                     /locus_tag="IV334_001672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8943743.1"
                     /translation="MAKNSWELKINGHDELLVRMERYSSESERLINEALKSKGSAIAV
                     DRITEKIPVSEADLRRGHQHAKNSRPLKTQYINLGFIIRPTRKFEYLKYPDLGIGTSK
                     RNQPDEFMRRGLGLALDPITELLIRQFDKLNK"
     gene            8696..9304
                     /locus_tag="IV334_001673"
     CDS             8696..9304
                     /locus_tag="IV334_001673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAP8943744.1"
                     /translation="MAKTKTVVTTFDNVSIKRIAFNFKNAGNAIATNCNGQLDGETEM
                     QTVVKKCGATEVKSKSKPINMTVTITAHVPMEVYRRFNGLKQDGRIKPGIYSYGPDSV
                     GEDFSLAAEIVDDFEENRKLVGMLACTSNTGLTFSIENGADEVAALELETKVMQDEFG
                     KFYHEAIVAELEEDLTDQWMTNLSADVIKKTSVVTTTATQSQ"
     gene            9324..9686
                     /locus_tag="IV334_001674"
     CDS             9324..9686
                     /locus_tag="IV334_001674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8943745.1"
                     /translation="MNEDYSKIELNDGTILNLEPKLNIKKLLMINRDFNTDEFAKMTV
                     GKGSMDISVIQGAKAVYIAYRQANMTDYISFDEFIDKWDFDMATASYIYQLMMFKQAR
                     DAYQKEFEKANKEKKLQK"
     gene            9689..9871
                     /locus_tag="IV334_001675"
     CDS             9689..9871
                     /locus_tag="IV334_001675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8943746.1"
                     /translation="MPKLLVETWVDVYSMLTDVFSMPSDLVLSDICLDDILQMAYNKS
                     AYEGWKNYAINQSQKN"
     gene            9888..13307
                     /locus_tag="IV334_001676"
     CDS             9888..13307
                     /locus_tag="IV334_001676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HAP8943747.1"
                     /translation="MAKKRTEAEVTFIANDDGLKSTLKEISAELTKNRAELKLEQAQL
                     QQTGSESDKLGSKLSSLEKQYELQSQKVEVTSQRLANAKKYYGENSTEVQKLERELIN
                     QQTAQQRLSNEIDKTSNTLAQAKGEIQTYESTMQQLDSEQKNVQASASLIESEYKKWQ
                     VTAGQSASESEKLAKAQEYVSQQSENAEKTIDILRRQLEATQSEFGATSTEAMQMEAK
                     LNDAEREFEELGQAAKNVDTTNLDDIGSKIDMNNLMEASDVLSDIGDKLTELGKQAVD
                     SANSVGSSQSKIQANFGLTKQEAEELTNVARDIYYKGFGESLDQSTDALILVKRNLGD
                     LNNQDLQNITEQAMVLENTMGADMDETLRGVNGLMVNFGLSAQDAMDLMVSGTQNGLD
                     KTHELGDNMAEYSQLWSQMGYSADETFGMLQNGLDAGAYNLDKVNDLVKEMGISLTDG
                     RFEQNMDMFSESTRKAFEEWKNGGGTQKDVINSMIQDFSNMDGQYDQLNKASTIWSAL
                     GEDNAMKVVQSLTDVNHTFDDVSGSAQKMNEDSTTPLQELNGKIAELKDSLAPIGNTI
                     IDALEPVIDFLGKMADAFNNLPQPVQDYAVAIGGLTAAFTLLMPIIVGFMALGGPTTL
                     IIGAVITAIAGVIAIIKNWGAITDWFKGIWSKFTDWLGGTWESIKEGASSVWDGVKET
                     WSGFVDWVQEIWQGVSDWFGELWSGLVEGASNIWQGVQETWQAFVDWVSNIWNGVKEV
                     WSIIWADIVGIVQIPWTLITSLIQAGINIIVGIFDVAGQLLGAAWQAVWTPISDFLKN
                     TWDTMTQWISIAWNGIVTTFHTIFDPVVAWWNGIWTSISTTASNIWNSISATASSIWN
                     SIKNTITSLVQAAATVIQNIWSTVSSWLGGIWNSISSTASNIWSSVTSSISNAINAAK
                     SAIQSVWNSISSWISGIWNGIKNTALNLWNGITSTISSKVNDGKNAISSGWSNLTGIV
                     SDIFNNVKSTIANIWEGIKKTVSAPIDWIRDKISSVFDNLNISIPHIPLPHFKLSGEF
                     NPLKGKIPTLGVDWYAKGSVFNSPNIIGVGESGPEAVLPLKRSVLQEIGDRILSSTSV
                     SSRTQAVQPVNNYEFNFTIDGNADEVTMKQTTQQIIDSITKVQNDNASAWR"
     gene            13358..14095
                     /locus_tag="IV334_001677"
     CDS             13358..14095
                     /locus_tag="IV334_001677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAP8943748.1"
                     /translation="MTDCIHSIIDGFPDYLHKLALAERPTIPSPKRQRVETSVLGRLG
                     GLVQDYSFEDMSFTLHYNYLEDVEDHQAFKQSFYIMRHWLNYAKKLEFSDDPNVYYVI
                     QTIDIGDAENDIVEWGEFDVNITAKPFARVQEDVPITVDKPQSFNLLNNSLEESFPKI
                     IITPSATSCQFILNDYVFSFEGLVAGTDVVIDSDLMLCYEEQSDGDILDRSNKMKTMQ
                     YPTLQVDINHFNCTGLSKIQIYRNGLR"
     gene            14105..16396
                     /locus_tag="IV334_001678"
     CDS             14105..16396
                     /locus_tag="IV334_001678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8943749.1"
                     /translation="MIDNLITIYDKNDANNLAEHLYDTQGLGALSDWLTATVSNKLNG
                     AEIFQGTYPISGTNADLIVEGRIIQCYVDENRAKQRLRIYYAKTSVIGNTIEVKAEPI
                     FNDIRKSVLNKYDSGTEKITATQAWQNAKALAKPAIPSQFSFSSLVDTLANVKIEKAN
                     FLEFFGGKEGSILDRFHGEFLKDNNTLHHEKRLGTDHKIKAIYTKNLTGLDLEIDAQS
                     VLVGVYPFISSSSEGEDEITLPEEVIFTDYVDDYPAGYVSFVDFKDKATDVATLREAA
                     KDWLKTNIDKQKPQVSGSIELVPLRHQRGYEKFVDLEKVSMGDGVDVYHPQLKVNMSA
                     RIVEYTFNVLTNSYDKLVVGNVKTNFLENTENNVSNLINDAIDQLKNGGEISDLINDI
                     VDHQTDMITGQNGGYVLLDPKEAPSRILIMDTPDKNTARNVLQINNAGIGFSKTGING
                     TYETAWTLDGGFNASFITAGEIVGITIRGTTLISDGTDYRTSIANGKMTWYSKKVNKD
                     IMELEARDYVSADAGIVSYTMKTGGGFMIRNPQGNLVFSTWDNGNNRPFLSFGAPNFR
                     YSNASYVTSGDGSSLSINGSAGNSWEFKVAGRTMKFTSDGMLTLPGCFFGSWEDGKLA
                     RFEQSTVQVYKDFTVRGTKNSTVPTEHYGQRLLNAYETPEYYFADYGEAVTGDDGKVR
                     VDIDPMFAETVNLSRYMTHVTPTELVLCAVTHEDIDHFIIETSKPNVLVRWNLVAHRL
                     GYEDIRLKEDTAYDSTVLDQKRF"
     gene            16420..>17006
                     /locus_tag="IV334_001679"
     CDS             16420..>17006
                     /locus_tag="IV334_001679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8943750.1"
                     /translation="MASSLYNLALDFSKELNYTKAIMARQGDKGITVTVKPFLNGLQM
                     DTSGGTFTLKGTTPSNRYVDSVATSVTSEEVTFSLDGTFMSEAGYYKHCYVEYRKDNQ
                     ILTTQDIIFFSLGVSDISQGQADEYVSQLEELIQKYNETFDAFMAEIEGRVDSLNKQI
                     TDLTGQAKTLQDKLDALKEEISKLGNLQVMYSNSI"
BASE COUNT         6081 a         2473 c         3573 g         4879 t
ORIGIN      
        1 taaaaatatt acgaaagggg tgagagtgca tggaagacaa aatgtattta agacaaaaag
       61 ttttcacaaa aaaagatatt gaaaatcaaa ttaatggatt tatacagaaa ttagaaaatg
      121 caaattctta tttgataaat aaggaactaa ataaagctta tgaccagtgg aaagaagtat
      181 atgatgagtt gaaattgatt caaaatgaaa caaaattagt acgaacggag aaaaagaatg
      241 aaaatagttt cttttttgat ggatatgcta tattgatgtt ggaaactgta gcaaaacaaa
      301 atataaaagc tcccaaaaaa gaactatctg acaatattga taacgcatta gccgagttaa
      361 ggtattacgt tatgcaaata aaagatgtta ggatcactcg ttgagtggtc tttttatttt
      421 gcacaaagga ggtaacaaca atgtatagac cacaatactt agaacagaag tatgaagtaa
      481 tcactgtgca aaatggtaac ggagagatag tacgaaagta tagaagacca ataaagagcg
      541 atacatataa acgaaaggaa agcaatgaag ttattccatt gtatggcaaa agaatagcta
      601 agcattaaat aagattgtga aaggagacgg gacatgaacg aggaattcta tagatggcta
      661 ttacagttga taagagaaga tcgtttggtt aagttctatc agtctcctaa atggcgcagg
      721 cttagagaga aagcgatgaa acgagatcac tatgaatgcc aagagtgtag aagactaggt
      781 aagtatcata gagtagagaa cgttcatcat ataaaggaag tcaaggatag acctgactta
      841 gctttagatt tagataatct tatttgttta tgtgttgaac atcataatga agttcatggc
      901 agatatctta cagcattgga taaacaagag aagaagatag aaagctttgc taacttcgat
      961 gcaagtgaaa ggtggtaagt gcatgatcat caatgataat ggcagagagt atgatacaga
     1021 aaagattgaa gagtattcat cttatactca gggattaatt aaacgtttga tatacgttcg
     1081 ctatgtaggt attagggatc tgttatcaga taactgttgt agtaaataca aagtgaatca
     1141 agtaagagaa gcgttgaata aagataataa cgtcgaaaga ataaaaaatg tttttggata
     1201 tagtattgaa gagattaatt attacattga cttcgctgaa gctttcattc cgatggtgag
     1261 ataacccccc cttaaaataa atcgcaaatt ttttgggggt gatgaaacgg agggggctgt
     1321 caggaaaaga gattttttcg aactttatca tgaaaggagg gctaaaatgt ttaaaaacga
     1381 attgtctcaa aatcgctaca gagaaaaatt acgccgctct ttaataagcc aattggaaag
     1441 tcagaaaaca aatattgagc cattcttaga taatgttgat cgttatatca gtttatggga
     1501 aacggcgata tcactggaag aagatatatc cgagaacggc attagactgg agaatggtaa
     1561 aaagaatgaa tcagtagcgt tgcttgtttc tgtcaacaaa caaatgggat tgatgttgga
     1621 taaacttgcc attactcctg aattggtagg tgaagcaaat gaatcaattc ctgagttata
     1681 agcatattga aaattggttc aaagctatag aagaaggcac tatcaaggta tgcaaagagc
     1741 aattattgct aaaaaattat ctagaagaaa gagtctttac tagagaagat atttacttcg
     1801 ataagcagat ggtagaggat tcaatcaata taccagcaca atactttcca ttcgaattaa
     1861 ttccgtggga aaaatttcta caatgtttta tttatggtgt tcgatggaaa aaagataaaa
     1921 cactagtgtt caatagatat ctttcattaa tgggacgtgg taatggtaaa actggttttg
     1981 cttcttggaa caacttcttt ctactaaccg ctaaacacgg tattaaaaat tatgatattg
     2041 atatctatgc caataatgaa agccaagcaa agactagttt tgatgatgta tttaaagtaa
     2101 ttaaagatca tcctgattta gataaaaaag tatttaaagc tacgaaggaa gttattcaaa
     2161 atatcgctac aaacagcaaa cttcgttata acacggcaaa tgctagaaca aaagatggga
     2221 agcgaccagg tgcaaaccgc tttgatgaaa ttcacgaaaa tgaagattat tcaatgataa
     2281 atgtggctac ttctggtggt ggtaaaattc gagattatag agaattttat gatacaacta
     2341 atggtcatgt tcgtggtggt ccgcttgatg acattataga agaatcaaaa atgattcttt
     2401 ctggagaact tggaattgac aaggatggag cagaattttc tagtttgttt ccatttattt
     2461 gtcgcttgga taacgataat gaagttgatg atcccgacat gtgggaaaaa gcttgtccaa
     2521 ctattaatta caatgcaaat ttaaaacgga aaatgtttca agaatactct caaatgcaac
     2581 gtaatgctgg tttaagactt acgttcatga ccaaacgaat gaacagacct atggaagata
     2641 cacgatttgc tgttgcttca tatgatgatg ttctgcatac gaaagaaaaa gaatttcctg
     2701 aaaaaatgga tgaagtgata ggaacagtcg attttgctga tagacgagat tttgccagcg
     2761 ttgggttgct aggaaaatac gataaagatg tttattttac acaacatact tttatccacg
     2821 aatcagccct tcgattacaa aacatcaaac gagaggttat agatatttct atagatcaag
     2881 gaaaatcaca gatcgttcat ggaaaaaata tagaagctga ttatattgta ggttggtttc
     2941 ttgaaatgag taataaatat tatattaaaa aaatcgctat ggatatgtac cgtgcaaaaa
     3001 tattgaagcc cgctttagaa gaagcaggtt ttactgtgga aattgttcga agcggatctg
     3061 ttacacatgg tatgttaaaa gatctggttg atgacctttt tattaatcaa cgtttatttt
     3121 ttggtgacga tgcgattatg cgttggtatt gcatgaatgt atatgaagag catatttcta
     3181 atggaaatat acgctatgaa aaaatagaac ctgaaactag aaaaacggat ggcttttttt
     3241 cattccttca tggtttgaat tttttagatg atatttatga ttctgctcct gtaacagtca
     3301 caaatagctc agtagaaaat acaggaactg gatttactcc tctagtattc taacttgaaa
     3361 ggaggtgaga aagtggggat ttttcaaaag gcggtaggat acttcacaaa aaaagcaacg
     3421 gttcctttag aagaatactt ttgtaaattg caagttgatt ttgtgtatcg aaaatttgcg
     3481 attgaaactt gtattgattt gattgcaaat gcgatgagta aagcggaatt caagtcatat
     3541 gaagatggaa aaaataaaaa gaatgatctt tactataggc tgaatgtagc tcctaataag
     3601 aaaaataatg caacagaatt tagaaaaaaa ctgatcagga gattaatatt ctacaatgaa
     3661 gtattgatcg tttctccgtc taataattct agcgaaatat ttattgcgga tagttgggat
     3721 gtcacagaat atgcattgaa agatgatgtg ttttctcaag tgcaaattaa caacatagtc
     3781 cttgatagag aatttctaga aagtgatgtt atctatataa aatacgcaga tcaacaaatt
     3841 aggcaactag tcgatgcgta ttatcaagcg tatgggaaac tcatttctag tgccatgaat
     3901 gtttacaagc gctctaacgc tcgtagatac gtactgaaag ggaatttatt ccgaccgcaa
     3961 gacaatacaa cacaagatca aatcaataaa atgatgacat cacaatttaa ggcttttatg
     4021 gaagctgata atgcaggtgc ggtatttcaa ttacaaaatg agtacacatt agaagatttc
     4081 agcggaaact tccaaagcaa ttcaagagat ataaaaaact taatagacga catctttgag
     4141 atgacagcag cagcgtttca cgttccgaaa aacctactaa agggagacat gagtgggtta
     4201 tcggatcaag tggacgcttt tttaatgttc gaaatcatac cgattgctga acttattcag
     4261 gatgcgttta acgctagtct ctatgaagta gaagaatact tgtcagggaa ttttgtacgt
     4321 gtggatacaa ctatgatcaa gattactagc ttcaaagatt tggttgacgc tattgatgta
     4381 ggcattagaa atggagtatt tacaatcaac gaaggaagag aacgcgttgg aaatgatcgc
     4441 tctgataagg cgatggcaga tgaaatattt ataactaaaa acaatcaaca agtatcgaaa
     4501 ggaggtgagg cgaatgacga caatgaaaac atttctagca gtaaagaatg aaggcgcagt
     4561 accgcaaatt tttattcagg gatttattgg ttctagttgg ttctttgaag ggaatactga
     4621 caagggaatc aaaaatattt tggatagtct aggtgatcaa gaagaaattg aagtagtaat
     4681 taattcaaac ggtggagacg tatttcaagg gattgctatt gggaacttac ttaagtcaaa
     4741 taaagcaaaa gttaacgttg tgattaacgg cttagccgct agtgctgctt caattatcgc
     4801 aatggctggc gatactataa aaatctacaa caatgcacaa ttgatgattc accgcgcttc
     4861 cacatacgga gaaggaaatg tcgatgactt ccgtacgatt gctgaccaac tggaatcaat
     4921 tgataaatcg gtaaaggctt catataaaac acgattcaat ggcacagatg aagcattgca
     4981 agaacttctt gaaaaagaat cgtttatgga tgcagaaaca gctttgagtt atggattggt
     5041 cgatgaaatt atcgatgcag aaaatagctc aggtactgaa gctaaaaaag aacaaagcgt
     5101 tgaagaaatt ttgaatgacg ttaaagaaaa aagagcagaa aaaattgctg catttacagc
     5161 agcattaaat aaaacatttg gacaaggaga tgtaaaataa tgacagttaa aaatttaaaa
     5221 ggtgtaacag ctgcaagcga ccaattgatg aaagctttta aagatggtaa cgaagaatct
     5281 tttagcgcag ctatggtaag cttatctaag gaaattcagg ataaaatttt agaagaagca
     5341 acagcaaaaa atcaagatca attagtatta atgaaccgtg gtcagcgtgt attaactaca
     5401 caagaaacaa aattctataa cgaagtagtg aaaaacgaag gttttgcagg ggtcgaagaa
     5461 ttagtgccag ctactgtatt tgaacgtgta tttgaagatt tagaacaatc tcatccacta
     5521 ttgcaaaaaa ttacttttgt taacacaact ggtgtaacag aatggattgt gtcacgtgga
     5581 gtcaatccag catggtgggg taaactttgc gaagctgtta aaaaagtttt agataatggc
     5641 tttgatgtaa ttaacatgaa gcagttcaag ctatcaggtt atattcctgt atgtaaggca
     5701 atgcttgatt taggtccagt atggttagat cgttatgtcc gtactgtttt agtagaatca
     5761 ttgagaattg cattagaaca agcaattgtt gatggtactg gtaaagatat gccagtcgga
     5821 atgatgcgtg acatgagcaa acaaactagc ggagaatatg ctgaaaaaaa agcagaacct
     5881 attacagctt tagatgctgt aactatgggc cgtttgatgg cgcgactatc aaaattcaat
     5941 atcgaaggtg tgaatgatcc gatttatcgt aatgtgaatc cttctgatgt ggtcctaatt
     6001 gtgaatccaa ctgattactg gtctaaagta ttcccagcta agactgtact aactgctaat
     6061 ggagaatacg tacaagtatt gccagtacca gtttcagatt tgcagtcaac ggctgtgcca
     6121 gaaggaaaag cagttattgg ggtagcctca gattacttca tgggtgtagg atctacacta
     6181 aaaattgaag cttcagatga ataccatttt gttgaagacg aacgcattta tctagctaaa
     6241 caatatgcaa acggtcaacc taaacgtaac gatagtttca ttgtattaga tattagcgct
     6301 ttgggaacta ctactacaac tacaaaacca acaaccacaa caactacaac acaagcgtag
     6361 gtgatcagaa tgaagtatat tctttgtcag ccggcaatca atcggtttaa atgggagctt
     6421 gaagtttgtt taactaatct gaagaaacta ggaatcaaag atatcgtatt gcttttcagc
     6481 agacacgatg atcagattcc tatttttttt gagaaggaat atggtgttga agttcatgtg
     6541 tacgatgatc tgcgggacga caaagagtat attccttcga ttaaaccata tttatggtgg
     6601 aaatatttag aagaagatca ttcgcgtgag gacgaccgat atttctatat cgattcggat
     6661 gtcattttca ataaaagaat taatttgcgc aaattgcctt ctaaagatga tgtttggtat
     6721 tgtagcgact gctgtagtta tctaagtctt gattatatta gaagctgtga aaacggagaa
     6781 aatattctaa aagatatggc aaacattgta aatgttacag tagaatcttt ggaaactata
     6841 aacactaatt caggaggcgc acagtgggtt attaaccgtc ctaaagcgaa ttattggaaa
     6901 aaggtttatc tggattctaa tcggctatat cgctacctta gagggcaaaa aacaaatata
     6961 caaatctgga cagccgagat gtgggcacgg ctttggaaca tgatgtattt caatattggt
     7021 cctaaagttc acgaggaatt agacttttgt tttgctactg atccaataga aaaagttaaa
     7081 gaagtaaaaa tcttacacaa tgctggagta acaacaaacg atgaagattt atttttcaaa
     7141 gggagatacg tgacttctac gccttttgat gaagatttat catttgtaaa caagaaaaaa
     7201 tgctcttacg catatgttaa agcaattaag gcggtggtta gatgacgcct gaacaagtga
     7261 ctgaagaatt gctaatagct gtgaaggata atatttacgt tacctggaac gaagaagatg
     7321 agtcaattaa aaagatgata gctaaaaatg ctgtttatct tcaaagtaaa gtgagtacaa
     7381 cactttcttt ttctcctgaa agcttagaat acggattgct aatcgaaaga tgtagatacg
     7441 actggaatcg tgctttagat gagtttgaac aaaatttcgc tagtgagtta ttaggtttca
     7501 ttcaacatta tgcgctacaa gaatatattg caggtgatgt gaatggcgaa taatcgtaga
     7561 ctcgaagaaa cattcaacga tggttggtta aagattttga cgcaaactac caaaagaaat
     7621 gaactaggaa aaaagattgg tgtagaagat acagaaatca cttctttaaa atttagaaat
     7681 ctttccatga gagatagtga tataacagct atggatgcga tgggatcgaa attaactaag
     7741 aaagtaaaga ctccatttca tccaatcgcc aagaaattta ataaagatca atattttatc
     7801 gtaatcgata gtatgcgtta caacgttatc tatgccgatt acgataattt ttatatctat
     7861 ttttatcttg aaagtgtggg tgaatatggt gattgataat tctaaagaaa aagaacgttt
     7921 aaataagcaa atttctgcta tcaaaacttc cttagaagag cattttggcc tcaaactctt
     7981 tcaagactcc gtaggcgagg atgagctacc tgatgatttt aattacttca ttctcgaaac
     8041 aggagaaata gaaatgatca ctgagccaaa atatagcgtg ggtcaaaatc tatatctaac
     8101 tttctattca gaaaatagag aagatttaac aggagactca ctagatatta tttcattgat
     8161 tcaaaatcgt tcgattcgtt ttcagagaat ggatcccaat catttaaaac tagagaacca
     8221 agatcgctat atcgatcaat tggtatttac gtttagacga ttattgaaga gtgattgtca
     8281 tggctaaaaa tagttgggag ctaaaaataa atggacatga tgaacttctt gtgcggatgg
     8341 aacgctattc aagcgagagc gaacgactga ttaacgaagc attgaaatca aaaggttcgg
     8401 ctattgcagt ggataggatt acagaaaaaa ttcctgtttc tgaagcagat ttaagaagag
     8461 ggcaccaaca cgcaaaaaat agtcgtccac ttaagactca atacattaat ttgggtttca
     8521 tcattagacc tacaagaaaa tttgagtatt taaaatatcc tgatttgggg ataggtactt
     8581 ctaaaagaaa tcagccggac gaatttatga gaagaggatt aggtcttgca cttgatccaa
     8641 ttacagaact tctgattcgt caattcgata aattaaataa atagggggaa caacaatggc
     8701 taaaacaaaa actgtagtaa caacgttcga taacgtaagt atcaaacgaa ttgcttttaa
     8761 ttttaagaac gcaggaaatg caatcgcaac gaattgtaat ggacaattag atggcgaaac
     8821 agaaatgcaa acggtggtta aaaaatgtgg agcgacagaa gtaaaatcaa aatctaaacc
     8881 aatcaatatg acggtaacaa ttactgcaca tgtaccgatg gaagtttatc gacgtttcaa
     8941 tgggttgaaa caagatggac gtattaaacc aggcatttac tcttacggtc ctgattccgt
     9001 aggcgaagat ttctcacttg ctgcagagat cgtggatgac ttcgaagaaa atagaaagtt
     9061 agttggtatg ttagcatgca cttcgaatac aggattaaca ttctctattg aaaatggtgc
     9121 ggatgaagta gctgcgttag aactagaaac aaaagttatg caagatgaat ttggtaaatt
     9181 ttatcatgaa gcaattgttg cagaacttga agaagactta acagatcaat ggatgacgaa
     9241 tctatctgct gatgtgatta aaaaaacttc agttgtgaca actacggcca ctcaatcaca
     9301 gtaaaaaaaa cggaggtagc gaaatgaacg aagattactc aaaaattgaa ctaaacgatg
     9361 gaacaatttt gaatttagaa cctaaactga atatcaagaa attattgatg atcaatagag
     9421 attttaacac agacgagttt gcaaaaatga ctgtgggaaa aggatccatg gatatttctg
     9481 ttattcaagg tgcaaaggct gtgtatattg cttaccgcca agcgaacatg actgattata
     9541 tttcattcga tgaatttatc gataaatggg attttgatat ggctactgcc agctatattt
     9601 atcaattgat gatgttcaaa caagcacgcg atgcttatca aaaagaattt gaaaaagcaa
     9661 ataaggaaaa aaagcttcaa aagtaaaaat gccaaagctc ttagttgaaa cgtgggtcga
     9721 tgtctattcg atgttgaccg acgttttttc tatgccttca gatttggttt taagcgatat
     9781 ctgtttagat gacattttgc aaatggctta caacaagagt gcttatgaag gatggaaaaa
     9841 ctatgcaata aaccaatctc agaaaaacta aagaaaggag gtaaaaaatg gctaaaaaga
     9901 gaacagaagc agaagtaact ttcatagcta acgatgacgg attgaaatct acgttaaaag
     9961 aaatcagtgc tgaattaact aaaaatagag cagaattaaa actagaacaa gctcaattac
    10021 aacagactgg ttctgaatca gacaagttag gaagtaaatt atcttcttta gaaaagcagt
    10081 atgaattaca aagccaaaaa gttgaagtaa ctagccaacg tttagccaat gccaaaaaat
    10141 attatggaga aaattccacc gaagttcaga aacttgagag agaactgatt aaccaacaaa
    10201 cagcgcaaca acgtttgtca aacgaaattg ataaaacgag taatacacta gctcaagcaa
    10261 aaggcgaaat acagacgtac gagtctacaa tgcaacagtt ggatagtgaa caaaaaaatg
    10321 ttcaagctag tgcttctctg attgaatccg aatacaaaaa atggcaagta actgctggtc
    10381 aatcagcttc tgaatccgag aaattagcga aagcccaaga atatgtttct caacaatctg
    10441 aaaatgcaga gaaaacgata gatatcctga gacgacagtt agaagctaca cagtctgagt
    10501 ttggcgctac atccacagaa gcaatgcaga tggaggcgaa gcttaatgat gctgaacgtg
    10561 aatttgaaga gttaggacaa gctgctaaaa atgtagatac aactaacttg gacgatatcg
    10621 gaagcaaaat agatatgaat aatttaatgg aagcttctga cgttttaagc gacattggcg
    10681 ataagcttac agaattaggg aaacaagcag tggactctgc taacagtgta ggtagttccc
    10741 agagtaaaat acaagctaat tttggtttga ctaaacaaga ggctgaagaa ttaacgaatg
    10801 tagccagaga catttattat aaaggttttg gagaatcgtt agatcagtcc acagatgcat
    10861 tgattttggt aaagcgtaat ttaggcgatt taaataatca agatttacaa aatattacgg
    10921 aacaagctat ggtcctagaa aacaccatgg gcgctgatat ggatgaaacg ttacgtggtg
    10981 taaatggctt aatggtcaac ttcggattga gcgctcaaga tgcaatggat ttaatggttt
    11041 cgggtactca aaacggttta gataaaacgc acgaattagg cgacaatatg gcagaatata
    11101 gccaattatg gagtcaaatg ggatattcag ctgatgaaac gttcggaatg cttcaaaatg
    11161 gtttagatgc gggtgcttat aaccttgata aagtcaatga cttagttaag gaaatgggaa
    11221 tatcgttaac agatggtcga tttgagcaaa acatggatat gtttagtgaa agtactagaa
    11281 aagcttttga agagtggaaa aatggtggag gaacacaaaa agacgttatt aattccatga
    11341 ttcaagattt tagcaatatg gatggtcaat acgaccaatt aaataaagct tctacaattt
    11401 ggtctgcact tggcgaagat aacgcgatga aagttgtcca atctttgact gatgttaacc
    11461 atacatttga tgatgttagt ggatctgcac aaaaaatgaa tgaagattct actactccgt
    11521 tacaagaatt aaacggaaaa atagctgaat taaaggattc attagctcct ataggcaaca
    11581 caatcataga tgcactcgaa ccagtaattg attttctagg aaagatggct gatgcgttta
    11641 ataatcttcc acaaccagta caggattatg ccgtagcgat tggcggattg actgctgcat
    11701 ttactttatt aatgccaata atagttggct tcatggctct aggtggtcct actacattaa
    11761 taataggagc agttattact gctattgctg gagttatagc aattataaaa aactggggcg
    11821 caattactga ctggtttaag ggaatatgga gtaaattcac tgattggttg ggtggtactt
    11881 gggaaagcat aaaagaaggt gcctcatcag tttgggatgg agtcaaagaa acctggtctg
    11941 gatttgtaga ttgggttcaa gaaatttggc aaggagtttc tgattggttt ggagagttat
    12001 ggagcggatt agttgaagga gcttccaaca tctggcaagg agtccaagag acttggcaag
    12061 cattcgttga ttgggtttca aatatttgga acggagtcaa agaagtatgg tcgattattt
    12121 gggcagacat tgtaggaatt gttcaaatac catggacatt aataacgtca ttgattcaag
    12181 ctggtattaa tattatcgtg ggtatctttg atgtagctgg acagttatta ggcgcagctt
    12241 ggcaagctgt ttggacacct atttctgatt tccttaaaaa tacttgggat actatgacac
    12301 aatggataag catcgcttgg aatggaattg taactacatt ccatactata tttgatccag
    12361 tagtggcatg gtggaatggt atatggacat ctattagtac tacggcttca aatatttgga
    12421 actcaattag tgcaacagct tctagtattt ggaatagtat caagaataca atcactagct
    12481 tggtacaagc agctgctaca gtaattcaaa atatttggtc aactgtatct agttggttag
    12541 gtggaatttg gaattcaatc agctctacag catcaaatat ctggagtagc gtgactagta
    12601 gtataagcaa tgctataaac gcagctaaaa gtgccattca aagtgtttgg aatagtatat
    12661 cttcgtggat cagcggaatt tggaacggta tcaaaaacac tgctttgaat ctttggaatg
    12721 gaattacaag cactattagc tctaaagtaa acgatggaaa aaatgcaatt tcaagcggtt
    12781 ggtccaatct aacaggtatt gtttccgaca tattcaataa tgttaaaagc acgatagcta
    12841 atatatggga aggtatcaaa aagactgtta gcgctccgat tgattggatt agagataaaa
    12901 tcagtagtgt ctttgataat ttgaatattt ctataccaca tattccgtta ccacatttta
    12961 aattgagcgg agaattcaat ccattgaagg ggaaaatccc aacattgggt gttgattggt
    13021 atgcgaaagg tagtgtgttt aattctccaa atattatcgg tgtaggtgaa tcaggacctg
    13081 aagcagtttt acctttgaaa agatctgtgc tgcaagaaat tggtgatcgt atcttgagta
    13141 gcacctcagt ttcatctagg acacaagcgg ttcaacctgt gaacaattac gaattcaatt
    13201 tcacaattga tggtaacgca gatgaagtca ctatgaagca aacaactcaa caaatcattg
    13261 atagcattac aaaagttcaa aatgataatg cttcggcatg gcgttaaaca ggagagtatt
    13321 tctcctgttt ttttagtatt aaaaaggatg tgaaaaaatg actgattgta tacattctat
    13381 aatcgatgga tttcctgatt atttgcataa attggcttta gctgaaagac caaccatacc
    13441 ttctccaaaa agacagagag ttgaaacttc tgttttagga aggttaggtg gcttagtgca
    13501 agattactcg tttgaagaca tgtcgtttac attgcactat aactatttag aggatgtgga
    13561 agaccatcaa gcgttcaagc aatcgtttta tatcatgcgt cattggttaa actatgcaaa
    13621 gaaattagaa ttctctgacg atcccaacgt ctattacgtt atccagacta tcgatattgg
    13681 ggatgcagaa aacgatattg ttgaatgggg agagttcgat gtaaatatta ctgcgaaacc
    13741 attcgcaaga gttcaagaag atgtacctat aaccgtagat aaaccacagt catttaactt
    13801 gctgaataat agtttagaag aaagttttcc aaagattatc atcactcctt cagctacttc
    13861 atgccagttc atcttaaatg attatgtgtt tagtttcgaa ggcttagtag caggaactga
    13921 tgtagtcatt gatagtgatt tgatgctttg ctacgaagag caatcggacg gagatatttt
    13981 agatcggtcc aacaaaatga agaccatgca atatccgaca ttgcaagtgg atatcaatca
    14041 ttttaattgc actggtttga gcaaaataca aatttatcgt aacgggttaa ggtaggtgaa
    14101 atagatgatc gataatttaa taactattta cgataaaaat gacgcgaata atttagctga
    14161 acatttatat gatacgcaag gtttaggcgc tttgtcagac tggttaacag ctactgttag
    14221 caataaacta aacggagccg agatatttca gggtacttat ccaataagcg gaactaatgc
    14281 agatttgatt gtagaaggac gtattattca gtgttatgta gatgaaaatc gagcaaaaca
    14341 gcgtctacgg atttattatg caaaaacttc tgtaataggc aatacgatag aagtaaaagc
    14401 tgaacctatt ttcaatgata taagaaaatc ggtgttgaat aaatatgaca gcggaacaga
    14461 aaaaatcact gctactcagg catggcaaaa cgcaaaagct ttagcgaaac cagctatccc
    14521 ttcgcagttt tctttctcgt cattagtaga tacgcttgct aatgtgaaga tagaaaaagc
    14581 gaatttttta gaattctttg gtggaaaaga gggatctatt ctagatcgat ttcatgggga
    14641 atttcttaaa gataataaca cattacatca tgaaaaaagg ctaggtacgg atcataaaat
    14701 caaagcgatt tatactaaaa atttaactgg tcttgactta gagatagatg ctcaaagcgt
    14761 tttagttgga gtttatccat tcattagcag ttcttcagaa ggagaagacg agatcactct
    14821 accagaagaa gtcattttca cggattacgt ggatgattat cctgctggat atgtttcttt
    14881 tgttgatttt aaagacaaag cgactgatgt agccacatta agggaagctg ctaaagactg
    14941 gttgaaaaca aacatagata aacaaaaacc acaagtgagt ggttcgattg aattagtacc
    15001 attgaggcat caaagaggct atgaaaaatt tgttgatcta gaaaaagttt cgatgggtga
    15061 cggagtagat gtgtatcatc cacagttaaa agtgaatatg tcagcgagaa ttgtggaata
    15121 tacgtttaat gttctaacca attcatacga taaattagtt gtaggaaacg tcaaaacaaa
    15181 cttcttagaa aacacagaga ataatgtaag caatttgatt aatgatgcca ttgatcaatt
    15241 gaaaaatggt ggcgaaatca gtgatttaat caatgatatt gtagatcatc aaactgatat
    15301 gattactggt caaaatggtg gttatgtttt attagatcct aaagaagcgc ctagtcgtat
    15361 tttgattatg gacacaccag ataagaatac cgcaaggaat gttttacaaa tcaacaatgc
    15421 tggtattggt ttttctaaaa ctggcattaa tggaacatat gaaaccgcat ggacattaga
    15481 tggcggattc aatgcctcgt ttattacggc tggtgaaata gtaggaatta ctattagagg
    15541 tactacatta attagtgatg gcactgatta tagaacaagt attgctaatg gcaaaatgac
    15601 ttggtactca aaaaaagtta acaaagatat tatggagcta gaagcacgtg attatgtaag
    15661 tgctgatgcc ggtattgtat catacaccat gaaaactggt ggtggtttca tgattagaaa
    15721 tccacagggt aacttggttt ttagtacgtg ggataatggt aataacagac cgtttctatc
    15781 ttttggtgcg cccaatttca ggtatagcaa tgctagttat gtaacttctg gcgacggtag
    15841 ttctttaagc attaatggta gtgcgggtaa ctcatgggaa tttaaggtag ctggtaggac
    15901 tatgaaattt actagtgatg gtatgctaac gttgccaggt tgtttttttg gttcatggga
    15961 agatgggaaa cttgctaggt ttgaacaatc aacggtacaa gtatataaag attttactgt
    16021 tagaggtact aaaaactcaa ccgtaccaac agaacattat ggacaacgac tattaaacgc
    16081 ttatgaaact ccagaatatt atttcgctga ttatggggaa gccgttacag gtgatgatgg
    16141 taaagttcgt gttgatattg accccatgtt tgctgagaca gtaaatctaa gtcgatatat
    16201 gacacatgtg acacctacag aactagtttt gtgtgctgtt actcatgaag atattgacca
    16261 tttcatcatt gaaactagta agccaaacgt attagttaga tggaatttag tggcacaccg
    16321 tctagggtat gaagatatta gattaaaaga ggatacagca tatgatagca cagtgcttga
    16381 ccaaaaacgt ttttaaaacg aagacaagga ggtatataaa tggctagcag tttatataat
    16441 ttggctctag atttcagcaa agaattaaac tacaccaaag ctattatggc tcgtcaaggt
    16501 gataaaggga ttacagtgac tgttaaacca tttttaaatg gcttgcagat ggatacgagt
    16561 ggcggaacat ttactttaaa aggaacaacg ccatctaacc gttacgtaga tagtgtggca
    16621 actagtgtaa ctagtgaaga agtcacgttt tctcttgatg gcacatttat gagtgaagca
    16681 ggatattata aacactgcta cgtagaatat agaaaagaca atcaaatttt aacaacgcaa
    16741 gatatcattt ttttctcact aggagtgtct gacatttcgc aaggccaagc cgatgaatac
    16801 gtttcgcaat tggaagagtt gattcaaaag tataacgaaa cttttgatgc ttttatggct
    16861 gaaatcgaag gtagagtgga cagcttaaat aaacagatta ctgatttaac tggtcaagct
    16921 aaaacactac aagacaagtt agacgctctg aaagaagaaa tttctaaatt aggtaacttg
    16981 caggtgatgt acagtaacag catcga
//
LOCUS       DACDKM010000056        16802 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20834_2016
            SAMN12640370-rid9891663.denovo.056, whole genome shotgun sequence.
ACCESSION   DACDKM010000056 DACDKM010000000
VERSION     DACDKM010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640370
            Sequence Read Archive: SRR10040891
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16802)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16802)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742835.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742835.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:04:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,012
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 29 of 75
            Pseudo Genes (incomplete)         :: 33 of 75
            Pseudo Genes (internal stop)      :: 19 of 75
            Pseudo Genes (multiple problems)  :: 5 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16802
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640370-rid9891663.denovo.056"
                     /isolate="E20834_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(245..1576)
                     /locus_tag="IV334_001680"
     CDS             complement(245..1576)
                     /locus_tag="IV334_001680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAP8943751.1"
                     /translation="MGKSSRKDIVFEKFGIVATKLGNQIHLRTLRDAFATFMPFMMLA
                     GFVTLINSVILEPTGFMGEIVDTSTLTTLQQIGTSIANGTLSITTLLVVAAVSYHMCA
                     SRNYTNHIAAVLVAISTFVVLTPMQMMFTPENADKAVEVTGVIPISYTGASGMFVGIF
                     VGLAATELFIKLSNNKKLQIQLSGNIPPAVLKSFNVLIPIIITITSFALLSFIIIQLF
                     NMDINKLITTIVTEPLSKITTGLPGFLLITSIANLFFGFGIHQAVISGSLLDPFLIQN
                     MQENMAAYANKEQIPHIINMAFKDTFAVMGGSGNTIALLIAIFIFSRRKDYKDFAKLS
                     VTPAIFNISEPIIFGLPIVFNISLIIPFVLAPIFSLTVAYFATAVGLINHVVVQIPWT
                     TPPVISGFLATAGDWRAAVLQILIIAASVFIYLPFLRIDEHVTTKLSENET"
     gene            complement(1725..2438)
                     /locus_tag="IV334_001681"
     CDS             complement(1725..2438)
                     /locus_tag="IV334_001681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016611336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAP8943752.1"
                     /translation="MKKIAKYMEIYLDIKKKIVNGEYKIGEKLPTGDELAVIYHTSKL
                     TVKKGVDLLVSEGVLRSRSGYGTEVVRTPIDNSRVLGPNEGLFSVVGEEHVDSEIHTF
                     SIELPSKKVADKLSISVKDYVYNIVRSRFIDCQPYSIEQTFMPLAVIPGLEPKHLKKS
                     VYAYITNELGLEIKTSHIRIKGDTANEFDAKILKIDPGEFMIEIDKVVALSSGKPFEY
                     SVSRHLYQDFVFEAVFVEK"
     gene            2613..4037
                     /locus_tag="IV334_001682"
     CDS             2613..4037
                     /locus_tag="IV334_001682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015508962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HAP8943753.1"
                     /translation="MTTMKKLTFPDDFWWGSAWSAEQAEGKGETGKAETIWDRWFKEQ
                     PYRFYQRIDSSITTDHIHQYKEDIELMKKTGHNSFRVSISWARMFPNGGTGKVNPKAI
                     SFYRKLFSEMNNNGIKVFANLYHFDMPATLQDKGGWESREVVDAYVHYAETCFKEFGD
                     LVCHWFTFNEPLGPILGTYLEDFHYPNLIDFKRGAQAAFFTILAHAKAIQVFKELALP
                     SKIGVILNLSPTFPRSQNPADVKAAEIADLFYTRSFLDPMVKGNFSKKLILLLQEYNQ
                     LPEDYTKEDLVCIYENTAQILGLNYYEPRRVKSRSSMVDTSSPFLPEWFFEPHIMPGR
                     RMNEYRGWEIYEKGIYDLCMDIKNHYGNIEAFISENGMGVADEERFMDDTGQIIDDYR
                     IEYIKDHLAYLWKAINEGCNIKGYHLWTFIDCWSWINAYKNRYGLVSLDLTTQKRTIK
                     KSGEFYKQLSDTNGFRYDVDLLVE"
     gene            4336..5043
                     /locus_tag="IV334_001683"
     CDS             4336..5043
                     /locus_tag="IV334_001683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HAP8943754.1"
                     /translation="MDEKLDIKTAIKDTLPTVFGYIGIGLAFGIVGKAAGFHPLVVTL
                     MSLLIYAGSAQFITVSMLASHSPLLSIVLSTFLVNSRMILMSMTIAPYFKKNRLLQNL
                     LIGTLLTDESFALGMNKLNYTGQKLNFRWMNTANWISYLTWVGSSLVGALLGNFITDP
                     KKFGLDFAIVAMFIGLLYLQVISDRNTSKRLQLILIGLTLILVYVGLIFIPSNLVILV
                     VTLIGCGLGVWIKHAFF"
     gene            5030..5353
                     /locus_tag="IV334_001684"
     CDS             5030..5353
                     /locus_tag="IV334_001684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HAP8943755.1"
                     /translation="MPSFEFTLLTILGCTLATWLSRILPFVLLKKFDLPQPLLEYLSF
                     VPIVIMSALWFSSLFTQNIGHLPQINLENALASVPTLLAAILSKSLLVIVLVGILSLS
                     LIRLL"
     gene            complement(5442..6479)
                     /locus_tag="IV334_001685"
     CDS             complement(5442..6479)
                     /locus_tag="IV334_001685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase family
                     protein"
                     /protein_id="HAP8943756.1"
                     /translation="MSEKELMKVVGFYEGLSIDDPKSFIDEKEQIPVPSSRDLLVKVN
                     AVSVNPVDTKLRQDNGIRNALRILGFDGVGKVVAVGDEVQKFSVGDRVFYAGTTTRAG
                     SNQEYQLVDERIVALAPKNLSDEEAAALPLTSLTAYELLFEKFGLTPEENANRGKKIL
                     VINGAGGVGSILNQLAHWAGLEVYATASPKNFEWLKKTGVDYPIDYHHDLSKSFRELG
                     IDQVEYVAVLFDITRYFDQIKHLVRPFGHVGTIVGIKEPLDIGVWKNQSISFDWEYMF
                     AKTDYNYEIETQGEALAHIAALANEGIIRSTLSKVYSDGMNAKNLKQATKDVETGHIL
                     GKVVVSGPFNG"
     gene            complement(6800..7783)
                     /locus_tag="IV334_001686"
     CDS             complement(6800..7783)
                     /locus_tag="IV334_001686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-dihydrouridine synthase"
                     /protein_id="HAP8943757.1"
                     /translation="MTTNFWKELPKPFFVLAPMEDVTDVVFRHVVKHAAAPDVFFTEF
                     TNSDSFCHPDGKDSVRGRLTFTEDEQPMVAHIWGDKPEFFREMSIAMAEMGFQGIDIN
                     MGCPVPNVAERGKGSGLILRPEVAAQLIEAAKAGGLPVSVKTRIGYTEMSEMEEWISH
                     LLHQDIANLSVHLRTRKEMSKVDAHWEVIPKIIALRDQIAPQTLITINGDIPDRQTGE
                     ELAEKYGVDGIMIGRGIFKNPYAFEKEPREHTEKELIGLLRLQLDLQDHYSEIIPRSI
                     TGLHRFFKIYIKGFPGANDLRVQLMNTKSTDEVREILDTFEKEHPTLFSEQ"
     gene            complement(8198..8968)
                     /locus_tag="IV334_001687"
     CDS             complement(8198..8968)
                     /locus_tag="IV334_001687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP8943758.1"
                     /translation="MFNLYFTALKSLAIKETNRYLRIWVQTLVPPVITTSLYFVIFGK
                     MIGGRIGDMGGFSYMEFIVPGLIMMSAITSSYANVSSSFFSQKFQKNIEEILVAPVPT
                     HVIIWGFVIGGVGRSILVGSLVTIISLFFVPLHVYSWFMVIITLLMTAILFSLAGLLN
                     GIFAQSYDDVSIVPTFVLQPLTYLGGVFYAISMLPPFWQAVSKVNPIVYMISGFRYGF
                     LGTIDVPVATSMIVLVLFIVVLYAVTWYLINKGRGLRS"
     gene            complement(8961..9896)
                     /locus_tag="IV334_001688"
     CDS             complement(8961..9896)
                     /locus_tag="IV334_001688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP8943759.1"
                     /translation="MANALEINNLKKVYGSGVEALRGIDLSVKEGDFYALLGPNGAGK
                     STTIGIITSLVNKTSGKVNVFGYDLDTDLVRAKQQIGLVPQEFNFNPFETVQQIVVNQ
                     AGYYGVSRKEAMKRSEKYLKQSNLWEKRNVRARMLSGGMKRRLMIARALMHEPRLLIL
                     DEPTAGVDIELRREMWEFLRELNENGTTIILTTHYLEEAEMLCRNIGIIQSGELIENT
                     SMKELLSKLQFETFIFDLAPYETKPVIEGYACTFEDEQTIAVEVERNQGVNGIFDQLT
                     RQGIKVLSMRNKSNRLEELFLKITEENNHTEEKNV"
     gene            complement(10212..11861)
                     /locus_tag="IV334_001689"
     CDS             complement(10212..11861)
                     /locus_tag="IV334_001689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HAP8943760.1"
                     /translation="MGQREFIVLIIIAAVILLVALDIFSRLKLKETVRSKWEKIPYQP
                     RFDKEESLKEAWLTEKKFRSWDSEIDDLTWYDLDMFEVFEGINSTYSSVGSEALYQRL
                     RSFDFGEDQQLEKLIAFYQENPQLREKIQYQFARLGKKDHNFAKQYLADGKSKKIGRV
                     GLFIFLGLLPLVGLGLLLLGQVLGIYLAIGGLVFNTVYYLVKKQTLETELNSMSYLVS
                     TISAANQLAKLPTPLQEELKQQTKPLRDIPKLGFSFRMKSGSEAELIFEYVNIMFMLP
                     FISYHFVLTKLEKHSTEAIKLWELLGELEVAAAILNYRTYMPITCRPEFTEGGVTAKD
                     IYHPLLKEAVVNPVNWQKNTLVTGSNASGKSTYVKSIAISCILAQTIQTAIAEQFTLQ
                     PGHVLTSMAIEDDLFEGDSYFVSEIKSIKRLLDQVASKERCYCFVDEILKGTNTIERI
                     ASSASVVRWLAEYPSLAFVATHDIELTEILKNYCENVHFEEQVTEGKGISFDFKLKEG
                     PSRTRNAIALLKVLDYPETLVETAKTEAALFDEKRQWHVLG"
     gene            complement(11978..14581)
                     /locus_tag="IV334_001690"
     CDS             complement(11978..14581)
                     /locus_tag="IV334_001690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HAP8943761.1"
                     /translation="MVNAHIIQHTHWDREWYFTNEDAIVLSDQVFTEVLDELERNPKV
                     NFCLDGQTSIVDEYLEINPQKLKVIQRLMKTGQLFVGPWYAQTDALLVDAESILRNLM
                     IGISDTILKYGDPMMVGYLPDTFGFNANLPTLLHQVEIDNFMCWRGLNFETMVPSPYF
                     IWKGLGDKFVYAMNFPFGYMTGMMTLDAIKDIPEFAKEKLDEAVEMLHMQGKNKDILV
                     PSGVDQKSMILNFDQVVEKLNKYSKFHNIISDYPSYVEVIRNKKELPEYKGELRQPVY
                     SRVHRSIGSVRTKMKIENFKLEQKILKRVEPLMLIAERNGIHISKGLLRRLWKKILQN
                     QAHDSIGGCVSDNVAEDIFHRIKEANEIADGIENLIVKRMADALKLKSNEVLVFNTTT
                     ENYSGSKIVHVVADSKKIHFKGSPYNIIEKEVYFPERKNAYRMIATGFEYFDEPAYYE
                     LDIKINVNVPALGYTVVEFEKNNEKLETAIEIDKTEISNNKYKLSFKDGQLNLIVGDR
                     QYLDFVHLIDSANDGDTYDYSPLEGDTELSLKLETAKVYKDSLQETLVVYGKAQLPKD
                     LKDRLSEKPEMEEISYEISFSLGESQIVEGTLKIHNTVYSHRMRLAINVLDKSEKSIA
                     QIQNGFIENTPDVIPPNWSETMVEKPVNLEIFDKSVSVAENDHYFTFYADGLKEYERI
                     ENQLIITLFSTTGELGKPNLAWRPGRASGDTTNEGHVMMETPLAQEIGEYKVTFGFNV
                     EEGRFNEFKVAKQAEKRLEQSISYQKQKLNVFIHRLDNKIWPLQNELDVPRKFSLIEL
                     PEDLIVSSIYPSYFHEQGFVLRLANPTEQEKIVPEAILSLGTVVNALENKQELTTIPP
                     YDYLSILINER"
     gene            complement(14593..15642)
                     /locus_tag="IV334_001691"
     CDS             complement(14593..15642)
                     /locus_tag="IV334_001691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HAP8943762.1"
                     /translation="MEFKKQGREILNHFQSGVSYMIPLVVAAGLLTSIAVIFGGTGVW
                     DQTDTFWGVLRMIGQTGLQFIVPMISAYIAYSIADRPGLAPAFITGMMCQNLGMGFIG
                     GMVAGLACGYLANALKKVKVPMQVISLKSIMLIPFVTTLVVGIILWYVLATPIQAMTT
                     GLTNWLEGMSGANAALMSAILGGMMAFDMGGPINKIAYAFGVASFSSGSFGPSTAMLL
                     GIAIPPFGMFLATILNKKLYDESERDNAKTAMIMGLVGITEGTIPFAVKDPLRVIPSI
                     VVGTAVACGLNGFFGVTQETMLSTFMAIPFVTNIPLYLVSIVIGGVVTALMVNALKSI
                     KYKKEQQVNSVEEGK"
     gene            complement(15668..15982)
                     /locus_tag="IV334_001692"
     CDS             complement(15668..15982)
                     /locus_tag="IV334_001692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIB"
                     /protein_id="HAP8943763.1"
                     /translation="MTKIVAVTACIAGIAHTYMAKANLEKFAKKKGLDIKVETQGAMG
                     IENKLSISDVSEADVVIFAVDTNVSERGRFDGKPILTVGTSEVVKDGEETIDEAIALI
                     NK"
     gene            complement(15998..16444)
                     /locus_tag="IV334_001693"
     CDS             complement(15998..16444)
                     /locus_tag="IV334_001693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIA"
                     /protein_id="HAP8943764.1"
                     /translation="MISEKININNIVIDLNASDKKSVVEKLSKLLETNGFVSDHKQFQ
                     KDVFDRENHMTTGIGKNIAIPHGKSLAVIESTVAVAKLKRSVEWGALDDQPVDLVFLL
                     AIKDTDKGTNHLRILAELSGRLMDDEFVKRIKDSRNKKELVKALSF"
     gene            complement(16445..>16802)
                     /locus_tag="IV334_001694"
     CDS             complement(16445..>16802)
                     /locus_tag="IV334_001694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAP8943765.1"
                     /translation="IITKKLVKENYAKEGILEGAIKREKLSSTAMNDFAMPHIEVDFI
                     KKPVICVYVNPEGIDWDGTLVHSVFFFALNEKVKPELNKIYETFNEIASNHELLANLK
                     TSSSFEKLVYILKEGL"
BASE COUNT         5070 a         3423 c         2769 g         5540 t
ORIGIN      
        1 taaaataacg attaataata ttaacagtct ttatttttca ccaccaaaaa cctatctttg
       61 catatactaa atagacgtat atacacagat aggtttgtct tgtgaaaaat aatgattatc
      121 cttaccaaaa aatgtttgtt tttctacatg ttcgtactta tcaaaaaata tgatccttat
      181 tgaaattact ggctatttat attccaacga cttcttgtgt taatttttgt tatctcatag
      241 taaattatgt ttcattttca cttaatttag ttgtcacgtg ttcgtcaatt cgtaaaaacg
      301 gtaaatatat aaacacactt gcggctataa ttaatatttg taaaacagct gctcgccagt
      361 ctccggctgt tgctaaaaat cccgatataa ctggtggcgt agtccaagga atttgcacta
      421 ctacatgatt aattaatccc acagcagttg caaaatatgc tactgtcaaa gaaaaaatcg
      481 gtgcaagcac aaagggaata atcagactga tattaaatac aataggcaat ccaaaaataa
      541 taggttcgct tatattgaat atagcgggag ttaccgataa tttagcaaag tccttataat
      601 cttttctacg actgaagata aatattgcaa tcaataaagc aatcgtattt cccgatccac
      661 ccattaccgc aaaggtatct ttaaaggcca tattgataat atgtggaatt tgctctttgt
      721 ttgcataagc tgccatattt tcttgcatat tctgaatcaa aaacggatct aacaatgaac
      781 ctgatattac tgcttgatgg ataccaaatc caaaaaatag attagcaata gatgtaatta
      841 acaaaaatcc tggtaaacca gttgtaattt tacttaatgg ctctgtaact atcgtggtaa
      901 ttagcttatt tatatccata ttaaacaatt gaatgattat aaatgacaat aaagcaaacg
      961 aggtaatcgt aataatgatt gggattaata cattaaaaga ctttaaaaca gcgggaggaa
     1021 tatttccgga tagctgaatc tgtagtttct tattattaga aagtttaata aataattctg
     1081 tagccgctaa tccaacaaat atacctacaa acattcctga agctcctgta taggatatag
     1141 gtatcacacc cgtcacttca acagctttat ccgcattttc aggagtaaac atcatctgca
     1201 taggtgttaa tacaacaaat gtcgaaatag caactaatac cgctgcaata tgatttgtat
     1261 aatttctgct tgcacacata tgataagata ctgctgctac aactaataat gtagttatgc
     1321 ttaatgtacc attggcaatt gaagtaccaa tttgttgtaa cgtggtcaat gtactagtat
     1381 cgacaatttc ccccatgaat cctgttggtt ctaagatcac tgaattaata agtgtcacaa
     1441 atcctgctaa catcataaat ggcataaatg tagcaaacgc atctcgcaaa gttcttaaat
     1501 ggatctgatt ccccaactta gtcgcaacta taccaaattt ttcaaataca atgtccttcc
     1561 tgctactttt gcccatattt tttctccttt atgcttattg taatcgatta ctttttccac
     1621 ccaaataata catcacttat caaaaaaagt aaatactttt aattaaatat atatatcttt
     1681 tttagtataa aaaaataagc tctgaaaaaa taattttccc gagcttattt ttcaacaaaa
     1741 acagcttcaa aaacaaagtc ttgatataaa tgtctcgata ccgaatactc gaaaggttta
     1801 ccagacgata gagcaacaac tttgtctatc tcaatcataa attcacctgg atctattttt
     1861 aatatttttg cgtcaaattc attcgcagta tctcctttga tacgtatgtg cgaagtcttt
     1921 atttccagac ccagttcatt tgtgatataa gcatatacag atttctttaa atgttttggt
     1981 tccaagcctg gtatgactgc taatggcatg aatgtttgct caatcgagta tggttgacaa
     2041 tcaataaaac ggcttcgtac aatattatat acatagtctt ttacactgat acttaattta
     2101 tcagctactt ttttcgatgg aagttcaatt gagaaagtat ggatttctga atctacgtgt
     2161 tcttcaccaa ccacgctaaa caaaccttcg tttggaccaa gaactcgtga gttatcaatt
     2221 ggtgttctga cgacttcagt cccataccca cttctacttc gcaaaactcc ttcggaaaca
     2281 agtaagtcta cgcctttctt tactgttaac ttgcttgtat gataaataac tgccaattca
     2341 tctcctgtag gaagtttctc accaatttta tactctccat ttacaatttt tttctttata
     2401 tctaagtaaa tttccatata cttcgcaatt tttttcaatg gtgaccctcc aatcaaaaac
     2461 atatcttcat ctatacttta acataaataa accgataaaa tccacatcaa aattatacac
     2521 acaataaaaa agatatatat atttaaacaa aactatttac tttaagttgt aagcggtgtt
     2581 atcattaagt ggaaacagga ggtaaaaaaa ttatgacaac tatgaaaaag ctaacttttc
     2641 cagatgattt ttggtggggt tccgcttggt cagctgagca agcagaagga aaaggagaaa
     2701 caggaaaagc agagactatc tgggatcgct ggtttaaaga acaaccttac cggttttatc
     2761 aacgaatcga ttcctctatt acaaccgatc acatccatca atataaagaa gatatagaac
     2821 taatgaaaaa aactgggcat aactcctttc gcgtttctat ttcttgggca cgaatgtttc
     2881 caaatggcgg taccggaaaa gtaaatccca aagccatttc tttctatcga aaattatttt
     2941 ccgaaatgaa taataacgga atcaaagtat ttgccaactt atatcatttt gacatgcctg
     3001 ctactcttca agataaagga ggatgggaat cacgtgaagt agttgatgct tatgttcatt
     3061 acgctgaaac ttgttttaaa gaatttggtg acttagtttg tcattggttt accttcaatg
     3121 aaccacttgg gcctatttta ggtacttatc ttgaagattt tcattatcca aacctcattg
     3181 actttaaacg cggagcgcaa gcagcattct tcactatact tgcacacgca aaagcaatac
     3241 aagtatttaa agaactagct cttcctagta aaataggcgt tattctaaat ttgagtccta
     3301 cttttcctcg tagtcaaaat cctgctgatg tgaaagcagc agaaatcgct gatttatttt
     3361 atacgcggag ctttttagat cctatggtta aagggaattt ttctaaaaag cttatcctgt
     3421 tattacaaga atataatcag cttcctgaag attatacgaa agaagatctt gtatgtatct
     3481 acgaaaatac cgctcaaatt cttggactaa actattacga gccacgtcgc gtcaaatctc
     3541 gttcttctat ggtggataca tctagtccat ttttaccaga gtggtttttc gaaccacata
     3601 tcatgcctgg acgacgaatg aacgaatacc ggggatggga aatttacgaa aaaggaattt
     3661 atgatttgtg catggatatc aagaaccatt atggaaacat cgaagcattt atctcggaga
     3721 acggaatggg cgttgcggat gaagaacgtt tcatggatga tacagggcaa ataattgatg
     3781 attatcggat tgagtatatc aaagatcatc ttgcttactt atggaaggca ataaatgaag
     3841 gatgtaacat taaggggtat catctttgga catttattga ttgctggtca tggatcaacg
     3901 cctataaaaa ccgatatggg ctggtttcac tcgaccttac tactcaaaaa agaacaatta
     3961 aaaaaagtgg cgaattttac aagcaattaa gtgatacaaa tggatttaga tacgatgtag
     4021 acttgttagt tgaataatat tctaaaattc cacttgaagt gaacacatta gaaaacaaga
     4081 ataatgagaa ataccaataa caaatacttt ttcttcatac ttcatttata cttgtcagta
     4141 taagagactg tgacaaatgt agtgatcctc agaagttagg tctggaaatc taacttgaga
     4201 agattttttt aaactacgtc cagtcttttt tatttgttta aaattgaagt tgacaagtct
     4261 tcattaaaaa tttagaatat tcacaagttt gacgacaatt atgaaaattt atgtcgtgct
     4321 aaaggagttt tgttattgga tgaaaagtta gatataaaaa ctgcgatcaa agacacgtta
     4381 cctaccgttt ttggttatat cggtattgga cttgcatttg gtatcgttgg gaaagctgcc
     4441 gggtttcatc cgctggtcgt cacgttgatg tccctgctga tctatgctgg ttctgcccaa
     4501 tttatcacgg tcagcatgct tgctagccat agtccactgc tctccatcgt tctctcgacc
     4561 tttctagtca attcccgaat gattctgatg agtatgacga ttgctcctta tttcaagaaa
     4621 aatcggctgc ttcaaaatct gctgattggc acattgctga cagatgaaag tttcgcactg
     4681 ggaatgaata aattgaacta tactggacaa aagttaaact ttcgttggat gaatacagct
     4741 aattggattt cttatttgac gtgggtgggt tcttctcttg ttggcgcact tttagggaat
     4801 tttattactg atccgaaaaa gtttggtttg gatttcgcca tcgtcgctat gtttatcggg
     4861 ttactttatc ttcaagtcat ttctgataga aacacctcta aacgattaca gttgatcctg
     4921 attggtctca ctttgattct ggtatatgtc ggactgattt ttatcccaag taacttagtg
     4981 attttagttg taacactgat tgggtgcgga ttgggggtgt ggatcaaaca tgccttcttt
     5041 tgagtttaca ttgctgacta ttttaggttg tactttagca acttggcttt ctaggatcct
     5101 accatttgtc ttactgaaaa aattcgattt gccgcaaccg ttgcttgagt atttaagttt
     5161 tgtcccgatc gtcattatgt ctgctttatg gttcagcagt ctatttaccc aaaatatcgg
     5221 acatctgccg cagatcaatc tcgaaaatgc tttggcttca gttcctacac ttttagctgc
     5281 tattctttca aaaagcttat tggtgatcgt cttggtagga attctttccc tttcgttgat
     5341 tcgcttatta taaataaaaa aacaagagct tatggcaaat tactgtcatg cgctcttgtt
     5401 tttatcaaag tcaaataaga aaaaaatcgg cgtattcttg cttatccgtt gaacggacca
     5461 ctaacaacga cttttcctaa tatatgtcct gtttctacgt ctttggttgc ttgtttcaag
     5521 ttcttcgcat tcatcccatc tgaatagacc ttgctcaatg tactgcggat aatcccttca
     5581 tttgctaaag cagcaatgtg cgctaaagct tctccttgcg tttcgatttc atagttatag
     5641 tctgttttag cgaacatata ctcccagtcg aagctgatag attggttttt ccaaacacct
     5701 atatctaacg gctctttgat ccctacgatc gttcccacat gtccaaatgg tcggacaagg
     5761 tgtttgattt gatcaaagta gcgggtaata tcgaataata cagcgacata ctctacttga
     5821 tcgattccta attcacgaaa acttttactt aagtcgtgat gataatcgat tggatagtct
     5881 acacctgttt ttttcagcca ttcaaaattc ttcggacttg cagtcgcgta gacttctagg
     5941 ccagcccaat gcgctagttg attcaaaatc gaaccgacgc ccccagctcc gttgatcact
     6001 aagatttttt tcccgcggtt ggcattttct tctggagtca gaccaaattt ttcaaataac
     6061 aattcataag cggtcaatga ggtcaacggc aaggctgcgg cttcttcatc tgaaagattt
     6121 tttggtgcaa gagcgacaat acgttcgtca accagttggt attcttgatt gcttcctgct
     6181 cttgtcgttg tacctgcgta aaaaacgcga tcgccgacag aaaatttttg cacttcatca
     6241 cctacagcaa caactttccc gactccatca aaccctaaga tacgtaaagc atttcttatc
     6301 ccgttatctt ggcgaagttt cgtatcgaca ggattaacag atactgcgtt gacttttacc
     6361 agaagatccc gtgaactggg tacaggtatc tgttcttttt catcaataaa gctttttgga
     6421 tcatcgatcg acaacccttc ataaaaaccg actaccttca ttaattcctt ttctgacata
     6481 cgaattcctc cttagcttca tcaagttgga gcatccatac agttttcatt tcaatatata
     6541 tagtatgcta tttgttccac ttttattcat cataattatt ctcttactta aagtaaacta
     6601 tttgcacttt tttggttaaa aaagaggttg cgacaagact tttgtcacaa cctcttttaa
     6661 acggtgttca atcaactgac cctcggtatt cgctcaaaaa acgacaaaat atgaggagct
     6721 atttttgttt ggctgttctt attacttgat gcaggacagc tttttcacga cctctaattc
     6781 acggtgttca agcaagaaat cattgttcac tgaaaagcgt tggatgttct ttttcaaagg
     6841 tgtctaagat ctctcgtact tcatcagtag atttcgtatt catcaactga actcgcaagt
     6901 catttgctcc tggaaatcct ttgatataga ttttaaagaa acgatgtagt cctgtgatgg
     6961 aacgcgggat gatttctgaa taatgatctt gcagatctag ttgcagacga agtagtccga
     7021 tcagttcttt ttctgtatgt tccctcggtt ctttttcaaa tgcataagga ttcttgaaaa
     7081 tcccccggcc aatcatgatg ccatctacgc catatttttc ggccaattct tctcctgtct
     7141 ggcgatctgg gatatctccg ttgatcgtga tcagtgtttg cggagcgatt tggtcacgta
     7201 aagcaatgat tttagggatg acttcccaat gtgcatctac tttactcatc tctttacgcg
     7261 tacgcaaatg gacagaaaga ttggctatat cttgatgaag taggtgactg atccattctt
     7321 ccatctcaga catttccgta tagccgatac gcgtcttgac gctgacaggc aaaccgccag
     7381 ctttagccgc ttcaatcaat tgtgccgcta cttctgggcg aaggatcaag ccactgcctt
     7441 ttccacgttc tgctacatta gggactgggc agcccatgtt gatatcgatt ccttggaagc
     7501 ccatttctgc catagcaatg ctcatttctc taaaaaattc tggcttgtct ccccaaatat
     7561 gggcaaccat cggctgttca tcttctgtaa aggtcaatct tcctcgtaca ctgtcttttc
     7621 catctggatg acagaaactg tctgagtttg taaactcagt aaagaagaca tccggtgctg
     7681 ctgcatgctt tacgacatgg cgaaatacaa catctgttac atcttccatt ggtgctaaaa
     7741 caaaaaaagg ctttggcagc tctttccaaa aattagtagt catttatttg ttactctcca
     7801 ttctatcaag cagtgaagtc gcaatctttc tcaaaaacat tgtcattata ctaggaaaca
     7861 aaaaaagaag caaagtgaac agcttaaaag atgtttctga ttgagcaaaa aaagactgtg
     7921 acaaaaatct tatcacaatt ccttttccgg aataaatggt gttcaaaagc ttgcatctgc
     7981 ggtaataagt ccaaccaaac gaaaaatagg aggagctatt tttgtttggc tgttcttatt
     8041 acttgatgca gaacagcttt ttcacaacct ctccactaaa cggtgttcaa tcagctgacc
     8101 ctcggtatta gctcaacaaa cgacaaaata tgaggaacta ttttcccgtt tgtttctcta
     8161 atactcaggt caaaacgctg atttcacaac ctcttttcta acttctcaat cctcttcctt
     8221 tattgatcaa ataccaagta acggcgtaca aaacaacgat gaatagcaca agtacgatca
     8281 tcgatgttgc tactggcaca tcgattgttc caaggaatcc gtaacggaaa ccggagatca
     8341 tataaacgat cggattgact ttcgaaacag cttgccaaaa tggcggcaac atagagatgg
     8401 catagaaaac gccacctaga taagttaacg gctgcaagac aaatgtaggc acgatcgata
     8461 catcgtcata agactgagca aaaatcccgt tgagcagtcc tgctaacgaa aacaagattg
     8521 ccgtcatcaa taatgtaatg atgaccatga accatgaata cacatgaagt ggtacaaaga
     8581 acaaggaaat gatcgttacg agagagccga cgagaatact gcgacccact ccgccaatca
     8641 caaagcccca aatgatcaca tgcgtaggaa caggcgctac caagatttct tcgatgtttt
     8701 tctggaattt ttgagagaag aaggacgaag aaacgttggc atacgaacta gtgatagcag
     8761 acatcataat caaaccaggc acgatgaatt ccatatatga aaaaccaccc atgtcaccga
     8821 tccgaccacc gatcattttt ccgaaaataa caaagtacaa ggatgtcgtg atcacaggcg
     8881 gaaccagtgt ttggacccag atacgcaaat agcggttcgt ttccttgata gctaaacttt
     8941 ttaatgcagt aaaataaaga ttaaacattt ttctcctccg tatgattatt ttcttctgtg
     9001 attttcaaaa acagttcttc taaacgattc gacttattcc tcatagaaag aactttgatt
     9061 ccttgtcttg tcagctgatc gaagattccg ttcacccctt gatttcgttc aacttcgaca
     9121 gcgattgtct gttcatcttc aaatgtacaa gcgtatcctt caatcacagg tttggtctca
     9181 tatggcgcta aatcaaagat aaatgtttca aattgcagtt tggatagaag ctctttcata
     9241 ctagtattct cgatcagttc acctgattgg atgatcccga tgttgcgaca aagcatttca
     9301 gcttcttcta aataatgcgt cgtcaaaatg atcgttgtac cattttcgtt caattctcgg
     9361 agaaactccc acatctcgcg tctcaattca atatcgactc ctgctgttgg ttcatccaag
     9421 atcagcaaac gtggttcatg catcaatgcc ctagcaatca tcagtcttcg cttcattccc
     9481 ccggaaagca tacgtgcacg gacatttctc ttttcccaaa gattggactg cttcaaatat
     9541 ttttcgctgc gtttcatcgc ttctttgcga gagacaccgt agtagcctgc ttgattcacg
     9601 acgatttgct ggaccgtttc aaagggattg aaattgaact cttgcgggac taatccaatc
     9661 tgttgctttg ctcggactag atccgtatcc agatcataac cgaagacatt cacttttcca
     9721 gatgttttat tgactaaaga agtaatgatt ccgatcgttg tagacttccc agctccattt
     9781 ggtcctaata gcgcatagaa atctccttct ttcacggaaa gatcgattcc tcgcaatgct
     9841 tcgaccccac ttccataaac ctttttcaaa ttatttattt caagcgcatt tgccatttat
     9901 ttcctatctc cttatagaca tgattcttta ttgtatcatg gaatacagca gatgaaaaaa
     9961 caacttggtt ccagcgaaaa atcaggttgc tgggctcagg aaaatctcct aatgaaagac
    10021 atcaatcgga cgaagtcaga caaccttttc agaaaaaaca aatcgaagat aaaggattac
    10081 tattcatatg tgcaacaaaa ggagcgtgag acaaaagtaa agaacacttt tgtctcacgc
    10141 tctaaacacg tataaacggc ggaagcaaaa gccaagattt ttgatccaat aaatactttc
    10201 gatcacttcg cctatcccaa tacatgccac tgccgctttt catcaaataa cgcggcttcc
    10261 gtttttgcgg tttctactaa agtctctggg taatccaaaa ctttcagcaa ggcaatcgca
    10321 tttcgggtcc ttgaaggtcc ttcttttaat ttgaaatcaa aactgattcc ttttccttct
    10381 gttacttgtt cttcaaagtg gacattctca caataattct ttaaaatctc tgtcaattcg
    10441 atatcatgtg tggcgacaaa tgctaaagat ggatactccg caagccaccg aacgactgaa
    10501 gcggaagaag caatccgttc gatcgtattt gtccctttta aaatctcatc tacgaagcag
    10561 tagcagcgtt ctttggaagc cacttggtcc aacaaacgtt tgatcgattt gatttctgaa
    10621 acaaaataac tgtcgccttc aaataaatca tcttcgattg ccatggaagt caacacatgt
    10681 ccaggttgca gggtaaactg ttctgcaatc gctgtttgga tcgtctgtgc tagaatacag
    10741 ctgatggcta tacttttgac atacgtagat tttcctgatg cattggaacc cgtgactaat
    10801 gtattcttct gccagttgac cggattcact accgcttctt ttaacaatgg atgatagata
    10861 tctttcgctg tcacaccgcc ctcagtaaac tctggtcggc aagtgatggg catataagta
    10921 cggtaattca atattgcagc ggctacttct aattctccca gcaattccca cagcttgatg
    10981 gcttcagttg aatgcttttc taatttagtg agaacgaaat gataggagat aaatggcagc
    11041 atgaacatga tgttgacata ttcaaaaatc aactctgctt cactcccact cttcatacga
    11101 aatgaaaaac ctaatttagg gatgtctctt aatggtttag tttgctgttt cagctcttct
    11161 tgaagtggtg tcggcagttt tgctagctga ttggcggctg aaatcgttga aaccaaataa
    11221 ctcatgctat tcagctctgt ttctagtgtt tgtttcttca ctagataata gaccgtattg
    11281 aatactaacc caccgattgc caaatagatt cctagtactt gtcctaaaag aagcaaaccc
    11341 aaacccacca gcggaagcaa gccaagaaaa ataaataaac ctacgcgtcc aatcttttta
    11401 ctttttccat ccgctagata ttgttttgca aaattatgat ctttttttcc taaacgagcg
    11461 aattgatatt ggattttttc tctcagttga ggattttcct gataaaaagc aatcagtttt
    11521 tctaactgct gatcttcacc aaaatcaaag ctgcgtaaac gttgatacaa cgcttctgaa
    11581 cctacactag aataggtact gttgattcct tcgaatactt cgaacatatc caggtcatac
    11641 caagtaagat catcgatttc gctatcccat gaacggaact tcttctccgt gagccatgct
    11701 tccttcaagc tttcttcttt atcaaatctc ggttgataag gaattttttc ccatttgcta
    11761 cgaactgttt cctttaattt caaacgtgaa aaaatgtcta aagcaactaa taaaataaca
    11821 gctgcaataa taattaaaac gataaattct ctttgaccca tatccctctc cgttccttta
    11881 tttgttagct ttagtatagc actgttgcgc tatttgaaaa gagggtaaaa aaacaccttt
    11941 caaaataaaa ggtgtttttt tcttgtatat tacatattta tctctcattt atcaaaattg
    12001 acaaataatc atagggagga attgttgtta attcttgttt attctccagt gcattgacaa
    12061 ctgtcccaag ggaaaggata gcctcaggta ctattttttc ttgttctgtt ggattagcta
    12121 agcgaagaac aaatccttgt tcatgaaaat aagaaggata aatcgatgat acaattaagt
    12181 cttcaggtaa ttcaatcaat gaaaatttac gaggaacatc aagttcattt tgtaatggcc
    12241 aaatcttgtt atctaacctg tgaataaata cattcagttt ctgcttttga taagaaatag
    12301 attgttctaa ccgtttttct gcctgttttg ccactttgaa ctcattaaat cttccttctt
    12361 ctacattaaa tccaaaagtg actttatact cacctatctc ttgagctagc ggtgtttcca
    12421 tcatgacatg cccttcattt gtcgtatctc ctgaagctcg cccaggtcgc caagcaagat
    12481 taggttttcc aagttcccct gttgttgaaa ataaggttat gattaattga ttttctatac
    12541 gttcatactc ttttaaacca tctgcataaa acgtaaaata atgatcattt tctgccacag
    12601 agacactctt atcgaatatt tctaaattaa caggtttctc taccatagtt tctgaccaat
    12661 tcggaggaat gacatcaggg gtattttcaa taaatccgtt ttgaatttgt gcaattgatt
    12721 tttcagattt atctaaaaca ttaattgcta agcgcatacg atgcgagtaa acagtattat
    12781 gaattttgag tgtgccttca acgatttgac tttctcccag actgaacgat atttcataag
    12841 atatttcttc catttcgggt ttttcagata aacgatcttt taaatcttta ggaagttgag
    12901 cttttccgta aacaactaat gtttcttgta aagaatcttt atatacttta gctgtttcta
    12961 attttagaga tagttctgta tctccttcta atggtgaata atcatacgta tcgccatcat
    13021 ttgctgaatc gatcaaatga acgaaatcaa gatattgtct atctccaact atcaaattca
    13081 attgcccatc tttaaaagaa agtttatatt tattgttact tatctcagtt ttgtctatct
    13141 cgattgctgt ttctaatttt tcattatttt tctcaaattc aacaacagtg tatcctagtg
    13201 ctggcacatt tacatttatt tttatgtcaa gttcataata agctggttca tcaaaatatt
    13261 cgaaacctgt agcaatcatc cgataagcat ttttccgttc tggaaaataa acttcttttt
    13321 caatgatatt atagggacta cctttgaagt gtattttctt tgaatcagct actacatgca
    13381 cgattttcga tccactataa ttctcagtcg ttgtgttgaa aacaaggact tcattcgatt
    13441 ttagctttaa tgcatcagcc atccgtttta caataaggtt ttcgattccg tcagctatct
    13501 cattagcttc tttgattcga tggaaaatat cttccgcaac attatctgaa acacagccac
    13561 caatactatc atgagcctgg ttttgcaaaa tctttttcca taaccgacga agtaggcctt
    13621 tactgatatg gataccattt ctttctgcaa tcagcatgag cggctcaact cgttttagaa
    13681 ttttttgttc caatttaaaa ttttctattt tcatttttgt tcttactgag ccaatagaac
    13741 gatgaacacg agagtaaacc ggctgacgaa gttcaccttt atattcaggt aattcttttt
    13801 tgtttctgat aacttctaca taagaaggat aatctgaaat aatattgtgg aatttactgt
    13861 atttattcaa cttctctacg acttgatcaa aatttaaaat cattgacttt tgatctacac
    13921 ccgaaggtac taaaatatct ttgttttttc cttgcatatg aagcatttca actgcttcat
    13981 ccaacttttc ttttgcaaat tcaggaatat ctttaattgc gtctaaggtc atcattccag
    14041 tcatataacc aaatggaaaa ttcatggcat aaacaaattt atctcccaat cctttccaga
    14101 taaaataagg agatggaacc atcgtttcaa agtttaatcc tcgccaacac atgaaattat
    14161 cgatttctac ttgatgcaaa agtgtaggca aattcgcatt gaacccaaac gtgtcaggca
    14221 aataccctac catcattgga tctccatatt taaggatcgt atcactgatt ccaatcatta
    14281 agtttcttaa aattgattca gcatccacta ataaggcatc tgtttgtgca taccacggtc
    14341 ccacgaatag ttgtcctgtt ttcatcagtc gttggataac cttaagcttt tgcggattga
    14401 tctccaaata ctcatcgacg attgaagttt gtccatctaa acaaaagttt actttagggt
    14461 tcctttccaa ttcatctaga acttcagtaa agacctgatc agacaatacg atggcatctt
    14521 catttgtaaa ataccattct ctgtcccagt gcgtgtgttg aattatatgt gcatttacca
    14581 tatcagattc tcctactttc cttcttctac tgaatttact tgttgttctt ttttatactt
    14641 gatggatttt agtgcattga ccattaacgc tgtaaccact cccccaataa caattgatac
    14701 taaatataaa ggtatattcg taacaaaagg aattgccata aaggttgaaa gcattgtttc
    14761 ttgagtaaca ccaaaaaatc catttagacc acatgctact gcagtgccca ccacgatgct
    14821 tggtattact cgtaaaggat ctttcacggc aaacgggatc gtcccttcag taatccccac
    14881 aaggcccata atcatagctg tctttgcatt atctcgttcc gattcatcat aaagtttttt
    14941 gtttaaaatt gttgctaaaa acattccaaa tggcggaata gcaattccta ataacatggc
    15001 tgtactaggt ccaaagcttc cgcttgaaaa agaagcaacc ccaaaagcat atgcgatttt
    15061 attgataggt cctcccatat caaacgccat cattcctcct aaaatagccg acatcaaagc
    15121 agcatttgct cctgacatgc cttctagcca attagtcagt cctgttgtca ttgcttgaat
    15181 aggcgtagct agtacatacc ataatatgat tccaacaact aatgtagtaa caaaaggaat
    15241 cagcataatt gattttagac taataacttg catcggtact tttacttttt tcaatgcatt
    15301 agctaaatag ccacaagcta atccagcaac cattcctcca ataaatccca taccaagatt
    15361 ttgacacatc atcccagtga taaaagctgg tgctagaccg ggacggtcag caattgaata
    15421 tgcgatatag gctgaaatca tcggtacgat aaactgtaat cccgtctgac caatcattcg
    15481 caggactccc cagaatgtat ccgtctggtc ccaaacacct gtgccaccaa aaatgactgc
    15541 aatagaagtt agtaaccctg ctgctacgac aagcgggatc atataggaaa ccccactttg
    15601 gaaatgattt agaatttctc ttccttgttt cttgaattcc attagtaact cccccacgat
    15661 atatttttta tttgttaatt aatgcaattg cttcatcaat cgtctcttca ccatctttta
    15721 ctacctctga ggtccctact gttaaaatcg gtttaccgtc aaagcgacct cgttctgaaa
    15781 catttgtatc tactgcaaat atgacaacat ctgcctcgga aacatccgaa attgaaagtt
    15841 tgttttctat ccccatagcc ccctgtgttt ctactttaat atctaaccct ttcttcttcg
    15901 caaacttttc taaattagct tttgccatat aagtatgggc aatccctgcg atacatgctg
    15961 ttactgctac tatttttgtc atatgaaatt cctcctttta aaatgataga gcttttacca
    16021 attctttttt atttctcgaa tcttttattc ttttaacaaa ctcgtcatcc atcaatcttc
    16081 cagataattc tgctaaaata cgtaaatgat tagtaccttt atcagtgtct tttattgcta
    16141 acaaaaagac gagatcaact ggttgatcat ctaatgcgcc ccattcaaca gaccgcttta
    16201 acttggctac agccactgtt gattcaatta ctgctaggct tttgccatga ggaatggcga
    16261 tatttttccc aattcctgtg gtcatatggt tttctctgtc aaatacgtct ttttgaaatt
    16321 gtttgtggtc agacacaaac ccattagtct ctaacaattt cgataatttt tcgactacac
    16381 tttttttatc agatgcattt aaatctatca ctatattatt tatattgatt ttttcactga
    16441 tcatttataa gccttctttc aatatataaa ctaatttttc aaacgacgaa ctcgttttta
    16501 aatttgctaa taactcatga ttcgaagcga tttcattgaa tgtttcataa attttattca
    16561 attcaggttt tactttttca ttaagtgcaa aaaagaaaac agaatgaaca agtgtcccat
    16621 cccaatcaat tccttctgga ttaacgtaga cacagattac tggctttttt ataaaatcta
    16681 cttcgatatg cggcatagca aaatcattca tcgctgttga agatagtttt tctcttttga
    16741 ttgctccctc tagtatccct tctttcgcgt aattttcttt taccaatttt ttggtaatta
    16801 ta
//
LOCUS       DACDKM010000057        16684 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20834_2016
            SAMN12640370-rid9891663.denovo.057, whole genome shotgun sequence.
ACCESSION   DACDKM010000057 DACDKM010000000
VERSION     DACDKM010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640370
            Sequence Read Archive: SRR10040891
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16684)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16684)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742835.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742835.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:04:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,012
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 29 of 75
            Pseudo Genes (incomplete)         :: 33 of 75
            Pseudo Genes (internal stop)      :: 19 of 75
            Pseudo Genes (multiple problems)  :: 5 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16684
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640370-rid9891663.denovo.057"
                     /isolate="E20834_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(<1..472)
                     /locus_tag="IV334_001695"
     CDS             complement(<1..472)
                     /locus_tag="IV334_001695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HAP8943766.1"
                     /translation="MVTRIFILDDSLVFEKMLVEILEESRILLSPWKFEIVQGLNVIQ
                     FVEFIKNNEIYSSDIFFLDIDLNAYYSGIELGEMLREKNEKCKIVYLTSHPNKAITVI
                     NRKITPSGYLIKEFTYEKNKKSIQSYLLKEKENYPVQHATKKRWTTVRYGNSDHY"
     gene            complement(720..1556)
                     /locus_tag="IV334_001696"
     CDS             complement(720..1556)
                     /locus_tag="IV334_001696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03943 family protein"
                     /protein_id="HAP8943767.1"
                     /translation="MLRFLILSGYFELMMYLQVSGKLDQYINVHYRYLAILSMILSAL
                     LALVQLYLWVKNGKAEEKAHEHDHDHGLSKPYQRGIAYVLLALPVIVGTLFPTVSLDT
                     TIVEAKGFNFPVSKESVGDPEMQTQYLKPDTSIYFNKSDYLDQMNELKEKYGDKQTIK
                     ITDENYLEVMELIYNYPSEFIGKKISYEGFVYQTPNDSNTDIFLFRFGIIHCVADSGV
                     FGLLTHMPEGVTVKNDEWYKIEGTIQSDYYQPFKREIPSVVVTNAEKVSAPKNQYVYR
                     SF"
     gene            complement(1567..2496)
                     /locus_tag="IV334_001697"
     CDS             complement(1567..2496)
                     /locus_tag="IV334_001697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HAP8943768.1"
                     /translation="MFSFIPNSVLQMSTIFLSIVIEALPFVLLGCLISGALQVFLTPE
                     RVKRWLPENRIFSIFTGSILGFFFPSCECGIVPIVHQFVKKGVPVHTAFAFMLTAPII
                     NPIVIFSTFIAFGNSWQMASWRVIGSFFVALVIGVWLAYFQKDAVLKEKITSKVAENH
                     HHHVHEEEQSEKNEPKNQLIHQTGHVLTHSIDEFFDTGRYLIIGGLIAAAMQTYLPTR
                     IMLTLGSTKILAIIIMLLLAFTMSLCSEADAFIGSSLLSLFGTAPVAAFLVFGPMVDI
                     KNLLMMKRYFTGRFILSLIGLIALSVIGFTLFI"
     gene            2688..3041
                     /locus_tag="IV334_001698"
     CDS             2688..3041
                     /locus_tag="IV334_001698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HAP8943769.1"
                     /translation="MKQTTNLTEVQDILQQSSVAVIYLSMPNCSVCHAVRPRLEELLT
                     HYDIPALHLDAFETPEVASKFEVLTAPVVLIFHQGKEVFRQARFIDFKKIRYLLDELT
                     AEDDSLSYEEIFRTK"
     gene            complement(3115..3771)
                     /locus_tag="IV334_001699"
     CDS             complement(3115..3771)
                     /locus_tag="IV334_001699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HAP8943770.1"
                     /translation="MKDHIIPKATAKRLPLYYRYLRILNNAGKKKVSSTELSEAVQVD
                     SATIRRDFSYFGELGKRGYGYDVEDLMHFFAKTLNDDQLTNVALIGVGNLGSALLKYK
                     FHQSNSIRISCAFDVNEEIVGRIVDGIPVYPMEDMSEQIKIQQIEVAILTIPAERAQE
                     VVNQLTEAGIKGILNFTAARLNAPSDVLIQNVDLTNELQTLIYFLHSDANAHSENTAE
                     "
     gene            3997..5931
                     /locus_tag="IV334_001700"
     CDS             3997..5931
                     /locus_tag="IV334_001700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HAP8943771.1"
                     /translation="MILLQANQIARHFGAEVLFENIHLEISTGARVALVGRNGAGKST
                     LLKIIAGIEAPDEGKIAKNKTATLGYLAQDTGLDSNETVWNEMLKAFEQVRQMEERMR
                     ELEIFISEGTPDTPAYSSYLKEYDRLQHDFSDRNGYGYENEIRSVLHGFKFDSSFYDQ
                     KIDTLSGGQRTRLALARMLLQHPDILILDEPTNHLDIETLSWLENYLQSYTGALLIVS
                     HDRYFLDKVVNEVYELSRRKMRHYKGNYSKYLDLKAEQLASEWKAYEKQQTEIHKLED
                     FVARNLVRASTTKRAQSRRKTLEKMDRLDRPQGDEKQANFLFSIEKPSGNVVLQIEDA
                     AIGYDNVTLSEPINLDIRRQEAIALVGPNGIGKSTLLKSIIHQIPFIRGKETLGTNVT
                     IGYYDQGQADLHTNKTILAELWDEHPTTPEKEIRSALGAFLFSGEDVEKPIPLLSGGE
                     KARVALAKLAMNKENFLILDEPTNHLDIDNKEVLENALIDYDGTLLFVSHDRYFINRI
                     ATKVVELSENGSKVYLGDYDYYLEKKQEEEEIAELLAQDEPESAPAPTNKSDYYQSKE
                     QQKLIRSLQRKITQVEEEMARIDEWIDQLEQEMVAPENLEDHVKLNELNQDLEAARQD
                     QETKLTEWEELSLELEEYEN"
     gene            complement(6017..7240)
                     /locus_tag="IV334_001701"
     CDS             complement(6017..7240)
                     /locus_tag="IV334_001701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronyl hydrolase"
                     /protein_id="HAP8943772.1"
                     /translation="MLKAGGEMLDKVMFEEIIHPEKWINECYKELMEKEWNNEIERCR
                     RHTERLIKRISNSQYQFPSECPIKGRYPLRDNIGWTEGFWTGINWLLFEKTGDPLFQQ
                     TAEKADIDFIHRIDCQIITNHHDLGFLYSLSTVADYKITGNRKMKEASIDAAETLLQR
                     FWEKAGVIQAWGDPSDPSQQGRMIMDCCMNLPLLYWASEETGNQKYKKAAQAHIQNAA
                     DYLVREDGSTYHTFYMDVKDGKPRYGSTHQGYSDNSCWARGQAWGIYGFALSYRYLKE
                     QKYFDLSKILANYFMNRLPEDTICYWDLIFDDSSREEKDTSSMVIMVCGLLELIESGL
                     LDKTEKNLYQSAVWKIMRQLKERYATTEEEDGFLTQSVYNKPKNNGVNTPCIWGDYYY
                     LEALVRMTSNWSSYW"
     gene            complement(7234..9132)
                     /locus_tag="IV334_001702"
     CDS             complement(7234..9132)
                     /locus_tag="IV334_001702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2264 domain-containing protein"
                     /protein_id="HAP8943773.1"
                     /translation="MVVKRGENTMKHPVPLLRTRQDYFDLFKKINQPLQPFYRASGAQ
                     IDYIHQGVGYGNKIAGMEGFSRVLWGAGPAIDQLDERWLKLILSGIKAGTDPSHPDYW
                     GEIHERDQRMVEMPAMLLALYHHEQLLWKKLDTGEKSQIIHWFSQIFEYECADGNWQF
                     FKIIVGTIIQKFGYPVKESALKEAFDNIENCYLQNGWYRDSSRGRQDYYNPFAFHYYG
                     LIYSVLEPEKPISQIYKNRAIDFSRDYIHFFAEDGANVPFGRSMIYRFAVSSFWSAML
                     WADLLPFKLGEIKGLVNRNIRWWMQKEIFDEKGILNIGYTYPQLTLTEPYNSTLSPLW
                     LNKIFLLLALPEDHEYWTAKEMPMPIREGTKLLSEANLLIQHDNGHTFFLNAGQLGPN
                     FHTLTNEKYLKFAYSSQFGFSIPRANQLKAEMAMDSMLGIQRADTYITTSFKNESIKT
                     YGQFIVRNNVRDIVCKKEVLASTWEPTLQAKIRTWLIPFEGWQIRVHKVELDTEYVLY
                     ETGFAIECSPEKEGIIIEEDKENYRVSEAGFSGIICLSDDTIKRKSTAIMGLPNTNLM
                     TWENTFIPGLEGTFGKGTHWLGTGVVAHQDRDYSLEVWNNRPKIDFDGTTGLTIQNKN
                     NKKRLKLC"
     gene            complement(9126..10244)
                     /locus_tag="IV334_001703"
     CDS             complement(9126..10244)
                     /locus_tag="IV334_001703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 88"
                     /protein_id="HAP8943774.1"
                     /translation="MNEQTKEWSRKILNVASEKIDAELDRMGNKIPYISYNGKYTDVI
                     KDRGIDWWTNGFWSGILWQLFHKTKREKFKEAAIQQEQLLESALYHFSDVHHDVGFLF
                     LPSAIAHYQQSQNKHSRQLGLHAATVLAGRYNISGNYLVAWNDHKPGWMIIDSMMNLP
                     LLYWASRETGDPRFSKIARQHADTTAKYLVRKDGSTGHIAEFDAESGKYIRLLGGQGY
                     NENSCWSRGNAWALYGFALSYRYTKNPYYLAIAEKIAVNFIQKIEKTGYIPLIDFAVK
                     DSNIDTSAGVCAACGLLELAGYVSSVRSKMYHEAAINILQKTEKHYANWNMDEDGIIG
                     GGAEAYHRELTYNVPLIYSDYFFIEGLLRLEQSELAIW"
     gene            complement(10327..11160)
                     /locus_tag="IV334_001704"
     CDS             complement(10327..11160)
                     /locus_tag="IV334_001704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAP8943775.1"
                     /translation="MTKQKIQSRIFTYVIIVFGALIFLMPYLYMIVASTQSNQEILSG
                     GINFKFGDMLLKNWQAIMARYNYPLIIWNSIVIAVISTVLATASSTMAGYALAKYRFM
                     GNKLLFNTVMLSRMVPFFTTLIPLFYIMSRLGLSNTYAGVIIPSIASTTSVFMMRQYS
                     SQFPTALMEAARIDGANEWVIFTKIAVPILSPTIITSGLLIFMGSWNQYLFPLVMLSD
                     KDKYTVPLIIKNMTVSSTGEPINYGAIMLILATSVIPMVLIYVWAQSKFKENDIGAAN
                     K"
     gene            complement(11153..12070)
                     /locus_tag="IV334_001705"
     CDS             complement(11153..12070)
                     /locus_tag="IV334_001705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP8943776.1"
                     /translation="MMQSSLPSKTIKNSKKKATDTSHKKSAYGLVTPFVIFFIVTILI
                     PIIISFAFSFMKIGYSFEFVGFDNYLAIFRDPLFIKSYTNVLIIMAGSIPITMLLAIY
                     FAVLLNSAQLKGKGFFRTVYYIPTITSTVAVASVFMTFFNPTGLFNGFLNALGINGVQ
                     WLTNPFWIRISMIITMIWLSVGYNTILFLAGLQGISKEIYESAEIDGASKFRQFFLLT
                     IPMLKPIILMATVLATINGLGNFNIPNIFFGTSNGPENSALVVGVNLYKTSFEMVDFG
                     KASAIAWTMVFVSVILSAIQFKFGGDKND"
     gene            complement(12297..13421)
                     /locus_tag="IV334_001706"
     CDS             complement(12297..13421)
                     /locus_tag="IV334_001706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HAP8943777.1"
                     /translation="MKKRYKLVIALLNITLLFSLSACGKTNEEVTANIADPMIKKATT
                     LEVYVANGKLTNYVENAAKIYNRKTGANIDLKIVNVASGTATVQMITPKLVTHEDMPD
                     IISMADTSAAGVLEKFQDAFYSADEYGFYDKYGDQFYDQKLNILRQQTSNETIIPWAS
                     DFTPALCYYQPKKFAEIGVDFEEISSWEEYIQVAKALKEKTGSFGIALPESGDQELFI
                     NMLAQQNQSLLDEEGNIRLNTKEAKHAAKLIKEMIDSDIVHFYGAQDAEKAFQESAMF
                     VAGGWYATNMSLNFPDASDKWRMAPLIPFSKENPGKGAVSGGSSFYVPKDAKNPQVAQ
                     QFLTFMLTDEECLANALELGVATSNSLAYQTEAAEKKFDY"
     gene            13604..14512
                     /locus_tag="IV334_001707"
     CDS             13604..14512
                     /locus_tag="IV334_001707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP8943778.1"
                     /translation="MVLLLTNNQSSNLVKKTLTYSIDTHLHLSAAPYFRLLINESANL
                     YAEFAHKSIELDNPSLLFIDKQQEVQIFSTQKETQTFIIIQFYFDEQLLAHITGQSFS
                     IDQEITSIITFLKNEQFKIDYLKHSKPAISYDQEINSFFILCQQMSIHLLSLLVTYCL
                     QKIQLSSLPRHYQQELALLTIPSNEKVIPKKKHILYSDELVDPIIRYLEEHLSENFLL
                     EQLAQIFFISPASLKKHFRKVNGKSVISYFKELKMEKAKRLVLENELTYTEIADILGF
                     HSIHHFSSAFKKYTGLSPSKYYLSLK"
     gene            14653..16266
                     /locus_tag="IV334_001708"
     CDS             14653..16266
                     /locus_tag="IV334_001708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-disulfide reductase"
                     /protein_id="HAP8943779.1"
                     /translation="MKVVVIGGVAGGPSFATRFRRLNEAHEIIIYERGENISYASCAL
                     PYYLGGVITDRDSLIERTPEILKTKNNIDVFTKHEVTAIDPSTKRLTVKDLSTNEEIK
                     TDYDKLIISSGARPDYPDIPGVFEAENGFVLRSVTDADRIKSFLEEKNPQHVVILGAG
                     VMGLELAENFKHRGLDVTLIDQLPQVAFPYDPEIANLVYDKLLKEGLAVHLETRVTEI
                     RDKGREIVLSDGSVLSADMLIFAVGVSPKNEVVKATGIQLSDTGQIIVDDQLQTNLPD
                     IYAIGDIIETTSVVTGQPIQSMLSSAANRQGHMLADILNGTPMRYRGYIGAGVAKIFD
                     HTASYAGMTEHELKASGITNYKTVFITPFDHAYFYPEATRLNLKLIFDADSGRILGGQ
                     AFGEKGVDKRMGELSVAITGNLTVFDLPDLELPYSPPYSTTRDPLNIAGYVAINQMTN
                     IVETIKASDIPENDLKEAFFLDIREPNKAPSGSISATKNIPMNELRDRINEIPKDKKV
                     YITFRRGLNTYTSARILAGLGIKAILIEE"
BASE COUNT         5280 a         3258 c         2768 g         5378 t
ORIGIN      
        1 tatagtgatc gctattacca taacggacag ttgtccacct ttttttcgta gcatgttgaa
       61 caggatagtt ttctttttct ttcaataagt aactttggat gcttttttta tttttttcat
      121 aagtgaattc ttttatcaaa taaccactag gcgtaatctt tcgattaata acagttattg
      181 ctttattcgg atgagacgtt agataaacaa ttttacactt ttcattcttt tctctaagca
      241 tttcgcctag ttcgataccg ctataatagg cgttcaaatc gatgtctagg aaaaaaatat
      301 ctgaggaata gatttcatta tttttaatga attcaacaaa ttgtataacg ttcaatccct
      361 gaacgatctc aaatttccaa ggacttaata aaattctgga ttcttccaaa atttctacta
      421 gcattttttc aaaaaccaaa ctatcatcca aaataaatat acgcgttacc ataaaaagta
      481 cctccactcg atattttact atcaaaatca tttcatacaa cttttcaaca tacatgttag
      541 gaataaattg taattctctt cacaatcttt tccaacacct ttgaaaccga tatcaaatac
      601 tttcaggatg ctgcttctaa aataaatatc aaacaaatga atacatcatg caaaaaaagc
      661 cagacaaccg acgtccttgc gtgtcagctg tctgagctat gtttatttat tttgttcttt
      721 taaaaagaac gatacacata ctgatttttc ggagcagaga ctttctcagc gtttgtaaca
      781 actacagatg ggatttcacg cttgaatggt tgataatagt ctgattggat ggttccttct
      841 attttatacc attcatcatt tttaaccgtt actccttccg gcatatgcgt caacaaacca
      901 aagactcctg aatctgccac acaatggata atgccgaatc ggaacaagaa gatatctgta
      961 ttgctatcgt tcggtgtttg gtaaacaaat ccttcatagc tgattttttt gccaatgaat
     1021 tcgctagggt aattataaat tagctccata acttctaaat agttttcatc tgtgatcttg
     1081 atagtttgct tatccccata cttttctttc aactcattca tttggtctaa gtagtctgat
     1141 ttgttgaaat aaatactggt atccggcttt aagtattgtg tctgcatctc cggatcccct
     1201 actgattctt tacttactgg gaaattaaat cctttggctt ctacgatcgt agtgtcgaga
     1261 ctaactgttg gaaacaacgt ccccacgatt actggcaaag ctagcagtac ataagcgatt
     1321 ccacgttgat aaggttttga caacccatgg tcatgatcgt gctcgtgtgc cttttcttct
     1381 gcttttccgt ttttgaccca caaatacagc tgaactaaag ccaaaagagc agataaaatc
     1441 attgaaagaa tagctaagta gcgataatgg acattgatat attgatcgag tttgccagaa
     1501 acttgcaagt acatcatcaa ttcgaaatac ccagataaaa tcaaaaaccg cagcatattt
     1561 tccctcctaa ataaataatg tgaatccaat aacgctaagg gcaatcagtc cgatcagcga
     1621 taaaataaag cgtcctgtaa aatatcgttt catcatcagt agatttttga tatcgaccat
     1681 tgggccaaag accaaaaagg cagctacagg tgctgtgccg aacagactta acaaagaaga
     1741 tcctataaac gcatctgctt ctgagcaaag agacatcgta aaggccaata acagcatgat
     1801 gataatggct aaaatctttg ttgagccgag cgttagcata attctagttg gtaaataagt
     1861 ctgcatcgca gctgcgatta atccgccaat gatcaaataa cgacctgtat caaaaaattc
     1921 gtcgattgaa tgtgtcaaca catgcccagt ttgatggatc agctgatttt ttggttcatt
     1981 cttctcactt tgttcttctt catggacatg atgatggtga ttttctgcta ctttagacgt
     2041 gattttttct ttcaatacag cgtctttttg aaaataggca agccagacac caatcacaag
     2101 ggcaacaaaa aagctgccta tcacccgcca gcttgccatc tgccaagaat tgccaaatgc
     2161 gataaaagtg gaaaaaatca cgattggatt gatgatcgga gcagtcagca taaaagcaaa
     2221 cgctgtgtga actggtacgc cttttttaac aaattgatga acgatcggta cgatgccgca
     2281 ttcacatgaa ggaaagaaaa atcctaagat cgaaccagta aaaatagaga agatacgatt
     2341 ttcaggaagc catctcttca cacgttctgg ggtcaaaaat acttgtaagg cgcctgagat
     2401 aagacagcct aataatacga aaggtaatgc ttcaataacg atcgataaga aaatcgtact
     2461 catctgcaaa accgaatttg gaataaaaga aaacatgagt ttcctccttt ttatttctag
     2521 ttcattcgct ttgcaaatat acgcttttat tcaaaaaaaa caagttgctc tgtaaatttt
     2581 tacacaactc ttatttttta ccaaagcact tcaattgact ttttctgtta ttcccattaa
     2641 attaaggtct catacatatc acttctatca taaaagggag aataaaaatg aaacaaacca
     2701 ctaacttgac tgaagttcaa gacatactgc aacagtcatc cgttgccgtc atttatcttt
     2761 cgatgcctaa ttgcagtgtt tgtcacgctg ttcgtccacg actagaggaa cttctcacac
     2821 attatgatat tccggcactt catttggatg catttgaaac accagaagta gctagtaaat
     2881 ttgaagtgct gactgcgcca gttgtcctga tttttcatca aggaaaagaa gtctttcggc
     2941 aagcacggtt catcgacttt aaaaaaatcc gttatctact agatgaactg acagcagaag
     3001 atgattccct ttcttatgaa gaaatcttcc gaacaaaata atcgttgtcc aaacagaaaa
     3061 aagcgattga acaagtgaaa gttttgcctt catttgttca atcgcttttt atttttattc
     3121 tgctgtattt tcggaatgcg catttgcgtc agaatgcaag aaatagatca aggtttgcaa
     3181 ttcattcgtc aaatcaacat tttggatcaa tacatctgac ggagcattca atcgagcagc
     3241 ggtaaagttc aagattcctt tgattcctgc ttccgttaac tgattgacta cttcttgtgc
     3301 tctttcagcg ggtatcgtta aaattgctac ttcgatttgt tggatcttga tttgttctga
     3361 catatcttcc attggataaa cggggatacc gtctacgatg cgcccaacga tttcctcatt
     3421 cacatcaaaa gcacaactga tacgtatgct attactttga tggaatttgt attttagtaa
     3481 agcacttccc aagttcccta caccgatcag cgcaacattt gtcaattgat cgtcgtttaa
     3541 tgtctttgca aaaaagtgca taagatcttc tacatcataa ccatacccac gttttcctaa
     3601 ttcgccaaaa taagaaaaat ctcgacggat cgttgcacta tctacttgaa cggcttcact
     3661 taattcagtt gaagacactt ttttctttcc agcattattt aaaatcctta aatagcgata
     3721 atacaaaggc agtcttttag ctgtcgcctt tggaataata tgatctttca cgaaatgacc
     3781 tccacgtatt gtctaattac atctttgatt tctccatcat agcagtattt ataggaaaaa
     3841 atcaattcca ttgctcagat attgtgaaat tttattcttt attcatttat ttatcatttc
     3901 tgcaaatctc gcagtataat ggaatttatg gtagactact aattgaaaaa tagtttttta
     3961 tttgaggaga attcacaaag tagaaaagag gattccatga tattattaca agccaatcag
     4021 attgctcgcc attttggtgc agaagtctta tttgaaaaca tacatttaga aatcagtaca
     4081 ggcgctcgtg tcgctttagt tggcagaaat ggcgctggaa aatcgacttt attaaagatc
     4141 attgctggaa ttgaagcacc tgatgaagga aagatcgcta aaaataaaac agcgactctc
     4201 ggatacctcg ctcaggacac aggacttgat tccaatgaaa cagtttggaa cgagatgctg
     4261 aaagcttttg aacaagttag acaaatggaa gagcggatgc gcgagctaga gattttcatt
     4321 agcgaaggta cgccagacac acctgcttat agttcttatt taaaagaata cgaccggctg
     4381 caacacgatt tttcagatcg aaacggctat ggttatgaaa atgaaattcg ttccgtattg
     4441 catgggttta aattcgattc ttcattttat gatcagaaaa tcgatacttt atcaggcgga
     4501 cagcgcacac gtttagcttt agctcgcatg cttttgcaac accctgacat tttgattcta
     4561 gatgaaccaa cgaaccattt agatatcgag acactttcct ggcttgagaa ttatctgcaa
     4621 agctatactg gtgcattatt gattgtttcc catgaccgtt attttttaga taaagtagtc
     4681 aatgaagtct atgaattaag cagaagaaaa atgcgccatt acaaaggtaa ctattcgaaa
     4741 tatctagatt tgaaagcaga acagttagca agtgaatgga aggcttatga aaagcagcaa
     4801 acagaaatcc ataaattaga agacttcgtt gctcggaacc ttgttcgcgc ctctaccaca
     4861 aaacgagcgc aaagccgcag aaagacatta gaaaaaatgg accggctgga tcgtcctcaa
     4921 ggggatgaaa aacaagcaaa ctttctattc agtatagaaa agccttctgg aaatgtagtc
     4981 ttgcaaatcg aagatgccgc tatcggatat gataatgtca ccttatctga accaatcaat
     5041 ctagatatcc gtcgtcaaga agcgattgct ctagtaggtc cgaatggtat cgggaaatcg
     5101 acgttgctga aatcaatcat tcaccagatc ccttttatcc gtggaaaaga aacgctaggg
     5161 acaaatgtca ccatcggcta ttacgatcaa ggtcaagctg atctgcatac aaacaagacg
     5221 atcctagcag aattgtggga tgagcatcct accactccag aaaaagagat ccgaagtgct
     5281 ttaggtgctt tcttattttc tggcgaagat gtagaaaaac caatccctct gctaagtggc
     5341 ggtgaaaaag cacgggttgc tttagccaag ttagcgatga acaaagaaaa ttttttgatt
     5401 ttggatgaac caacaaacca tttagatatt gacaacaaag aagtattaga aaatgcattg
     5461 atcgattatg acggcacact gctttttgtt tcccatgacc gatatttcat caatcggatc
     5521 gcgacaaaag tcgtcgaact ctctgaaaat ggcagcaaag tctatctagg agactatgat
     5581 tattatttag agaaaaagca ggaagaagaa gaaatcgccg aattgttggc acaagatgag
     5641 ccagaatctg ctcctgctcc cacgaacaaa tctgattatt accaaagcaa agaacagcaa
     5701 aagcttatcc gtagccttca acgaaaaatt actcaggttg aagaagaaat ggcgcggatc
     5761 gatgaatgga tcgatcagtt ggaacaagaa atggtcgcgc cagaaaacct ggaagatcac
     5821 gtaaaactca atgagctgaa ccaagattta gaagctgcta gacaagatca agaaacaaaa
     5881 ttgacggaat gggaagaact aagcttggaa ttagaagaat atgaaaatta acgaaaaaac
     5941 caaaacagaa accattcatg ctgttttggt ttttcttttt ttattttatc aaacaaaaat
     6001 tccatattta cctttttcac caataagaag accaattact agtcattctc actaaagctt
     6061 ctaaataata gtaatcaccc caaatacaag gtgtatttac cccattattt ttgggcttat
     6121 tataaacaga ctgtgttaaa aagccatctt cttcttccgt tgtagcatat ctttctttca
     6181 attgtcgcat aattttccaa acagcagact ggtataagtt cttttctgtt ttgtctaata
     6241 gacctgattc tattaattct aataatccac aaaccatgat caccatacta gatgtatctt
     6301 tttcttctct gctagaatca tcaaagatca aatcccaata acaaatggta tcttctggca
     6361 accgattcat aaaataattg gctaatattt tgcttaaatc aaaatatttt tgttctttca
     6421 aataacgata acttaatgca aaaccgtaaa tcccccatgc ttggcctcta gcccaacagg
     6481 aattatcact ataaccttga tgcgtacttc catatcttgg ttttccatct ttgacatcca
     6541 tataaaatgt atgataagtt gaaccatctt ctcttactaa ataatctgct gcattttgaa
     6601 tatgagcctg agcagctttt ttatattttt gatttccagt ttcttcactc gcccaataca
     6661 ataatggaag attcatgcaa caatccatga tcattctacc ttgttgtgaa ggatcactag
     6721 gatctcccca agcttgtata actcctgcct tctcccaaaa acgctgaagt aatgtttctg
     6781 cagcatcaat tgaagcttcc ttcatttttc gatttcccgt tattttataa tctgctacgg
     6841 ttgataaaga atacaaaaaa ccaagatcgt gatgattagt gataatttga cagtcaatcc
     6901 tgtggataaa atctatatct gctttttcag ctgtttgttg aaataaagga tcacccgtct
     6961 tctcaaataa caaccaatta attcctgtcc aaaatccttc tgtccaacca atattgtctc
     7021 ttaatggata acgtcctttt ataggacact cgcttgggaa ttgatactga ctgttactga
     7081 tacgtttgat taacctctcc gtgtgtctgc gacaacgctc gatctcatta ttccattctt
     7141 tttccattaa ttctttataa cattcattta tccatttttc tggatgtatt atctcttcaa
     7201 acatcacttt atcaagcatt tccccaccag cctttagcat aattttagtc tttttttatt
     7261 gtttttattt tggatagtta gcccggttgt cccatcaaaa tcaattttgg gacgattgtt
     7321 ccatacttct aaactataat cccgatcttg atgagcaacc actcctgttc cgagccaatg
     7381 ggttcctttt ccaaatgtac cctctaatcc aggaataaaa gtgttttccc atgtcatcaa
     7441 gtttgtattg ggcaaaccca taatagctgt ggattttcgt ttaatagtat cgtcacttaa
     7501 acagataata cctgaaaagc cagcttcact caccctatag ttttccttgt cttcttctat
     7561 gattattcct tctttctcag gagaacattc aatagcaaac cctgtctcat atagaacata
     7621 ttctgtatct aattctactt tgtgtacgcg aatctgccag ccttcaaaag gaataagcca
     7681 agttctgatt tttgcctgta aagtaggttc ccatgtagag gcaagtacct ctttcttaca
     7741 tacaatgtct cgtacattat ttcgaacgat gaactgtcca taggttttga tgctctcatt
     7801 cttaaaactg gtcgtgatat atgtgtccgc tcgctgaatc cctagcattg aatccattgc
     7861 catttctgct ttcaattgat tcgctcgcgg tatcgaaaaa ccaaattgac tagaataagc
     7921 aaattttaaa tatttttcat tggtcaatgt atggaaattt ggccctaatt gtcccgcatt
     7981 caaaaaaaat gtatgtccat tatcatgttg tatgagcaaa ttggcttctg aaagaagttt
     8041 agttccttca cgaataggca taggcatttc tttagccgtc caatactcat ggtcttcagg
     8101 tagcgccaac aataaaaaaa tcttattcag ccataatgga gataacgtac tattataggg
     8161 ttcagtcaat gtcaattgag ggtaggtata accaatattc aggatacctt tttcatcaaa
     8221 tatttctttt tgcatccacc atcttatatt ccgattgact aaccccttga tttcacctaa
     8281 tttaaaaggc aaaagatcgg cccacaacat agctgaccaa aagcttgaaa ctgcaaaacg
     8341 ataaatcatg cttcgaccaa aaggcacatt tgcaccatct tccgcaaaaa aatgaatata
     8401 gtctctagaa aaatcaatag ctcggttctt atatatctgg ctaatgggct tttctggttc
     8461 tagtacgcta taaatcaaac cataataatg aaatgcaaaa ggattataat aatcttggcg
     8521 acctcttgaa ctgtctcgat accagccatt ttgaagataa caattttcta tattatcaaa
     8581 tgcttctttc agtgctgatt ctttaactgg ataaccaaat ttttggataa ttgttccgac
     8641 aattatctta aaaaattgcc aattaccatc tgcacattca tattcaaaaa tttgcgagaa
     8701 ccaatgaata atctggcttt tttctcctgt atctaatttt ttccataata attgttcatg
     8761 atgatataac gctaataaca ttgcgggcat ttctaccata cgctggtctc tttcatgtat
     8821 ttctccccaa taatcgggat gggaaggatc tgttccagct tttattcctg atagaattaa
     8881 tttcaaccat cgttcatcta attggtcaat tgcgggcccc gctccccata aaacccgaga
     8941 gaatccttcc attcctgcaa ttttatttcc atatcctacc ccttgatgta tataatcaat
     9001 ttgtgcgcca cttgctcgat aaaacggctg gagtggttga tttatctttt taaataagtc
     9061 aaagtaatcc tgtctggtcc ttaatagtgg tactggatgt ttcattgtat tttctcctct
     9121 ttttactacc aaatagctaa ttccgactgt tctaatcgaa gtaagccttc aataaaaaaa
     9181 taatcagaat agatcagtgg cacattataa gtcagttctc tatggtatgc ttctgctccg
     9241 cctccaatga tcccatcttc atccatattc caatttgcat agtgcttttc tgtcttttgg
     9301 agtatattga tagcagcttc atgatacatt tttgatcgta cagaggaaac atatcctgct
     9361 aattccagaa gaccgcatgc agcgcatact ccagcactgg tatcaatatt cgaatctttt
     9421 acagcaaaat caatgagagg aatataacca gttttttcga ttttctgaat aaaatttact
     9481 gctatctttt ctgctatagc tagatagtac ggattttttg tgtaacgata acttaacgcg
     9541 aatccatata aagcccatgc atttcctctg ctccaacaag aattctcatt gtatccttgc
     9601 ccgccaagta accgaatata tttccctgat tctgcatcaa attctgcgat atgcccggtt
     9661 gatccatctt tcctcactag atacttagct gttgtatctg catgctgccg agcaatttta
     9721 gagaacctcg gatcaccagt ttccctcgaa gcccaataca gtaatggtaa attcatcata
     9781 ctatcaataa tcatccaacc aggtttatgg tcattccatg ctactaaata gttccctgaa
     9841 atattatagc gtccagcaag taccgtagct gcatgtaacc ctagttgtcg actatgtttg
     9901 ttttgagact gttgataatg tgcaatagcc gatggtaaga ataaaaaacc tacatcatga
     9961 tgaacgtctg aaaaatggta cagggcagat tctaataatt gttcttgttg tattgctgcc
    10021 tctttgaact tttctcgttt tgtcttgtga aagagctgcc ataaaatacc actccaaaaa
    10081 ccattggtcc accagtcgat tcctctgtcc tttattacgt ctgtatattt tccgttgtaa
    10141 gaaatatatg gaattttatt ccccattctg tctaattccg catctatctt ttcagaagct
    10201 acattcaaaa tttttcttga ccattccttc gtctgttcat tcatggatat cccgccattc
    10261 ttttaatttt tatgaatgtg gagttgctcc tagaacgaac tagatggtta acattcgtta
    10321 ttttttttac ttatttgcag ctccaatgtc attctcctta aatttagact gcgcccacac
    10381 ataaatcaag accattggga tcactgaagt tgctaaaatt agcataattg cgccataatt
    10441 aattggctca cctgtactag aaaccgtcat atttttgata attaagggaa ccgtatattt
    10501 atctttatct gaaagcatga ctaatggaaa tagatattga ttccaacttc ccataaagat
    10561 aagtaaacca ctagtaataa tcgtaggtga taaaatcggt acagcgattt tcgtaaaaat
    10621 cacccactca ttggcaccat cgattctggc agcctccatc agtgctgtag gaaattgact
    10681 gctatattgt cgcatcataa acactgaagt tgtactggca atcgaaggga tgatcacacc
    10741 tgcataagta ttcgataatc ctaaacgact cataatgtaa aataaaggaa tcaatgtggt
    10801 aaaaaaaggc accatcctag atagcataac tgtattgaac aacagtttat tccccataaa
    10861 acgatacttt gctaatgcat aacccgccat tgtagaactg gctgtagcta aaactgtact
    10921 gattacagca ataacaatcg aattccaaat aatcaaggga taattatatc tcgccatgat
    10981 ggcttgccag ttttttaata gcatatcccc aaacttgaaa ttgattccgc cacttaaaat
    11041 ttcttgatta ctttgcgttg aagctacaat catatataaa tatggcatca aaaatattaa
    11101 ggcaccaaac acaataatga cgtacgtaaa aattctgctt tgtatctttt gtttagtcat
    11161 ttttatcacc tccgaattta aattgaatag ccgataatat aacagataca aacaccatcg
    11221 tccaggcaat agcagaggct tttccaaaat caaccatttc aaaacttgtt ttatataaat
    11281 tgactcctac gaccaaagct gaattttctg gtccattgga agtcccaaag aatatatttg
    11341 ggatattgaa attacccaag ccattgatag tggctaaaac tgtggccatt aaaatgattg
    11401 gctttagcat ggggatcgtt aataaaaaga attgccgaaa tttcgatgct ccatctattt
    11461 cagcactttc gtatatctct ttagaaatcc cttgaagacc agctaaaaat aaaatagtgt
    11521 tatatcctac actaagccaa atcatcgtga tgatcattga tattcgtatc caaaaaggat
    11581 ttgttaacca ctgtacaccg ttaattccta gcgcattcaa aaatccattg aataacccag
    11641 tcggattgaa aaaagtcatg aatacggaag ctacagccac cgttgatgta atggttggaa
    11701 tataatagac agttctaaaa aatccttttc cttttaactg tgctgaattc aataaaacag
    11761 caaagtaaat agctaatagc atcgttattg gaattgatcc tgccatgata atcaaaacat
    11821 tcgtgtaaga tttaataaac aatggatctc ggaagatagc tagataattg tcaaatccaa
    11881 caaattcaaa tgagtagcca attttcataa aactaaaagc aaacgagata atgatgggaa
    11941 tcaaaatcgt cacaataaag aaaataacaa aaggtgtgac caatccataa gcactttttt
    12001 tatgagacgt atctgttgct tttttcttgc tgttctttat tgtcttgctt ggtaacgaag
    12061 attgcatcat cgttaagtcc tcctaatatt ttttaatcac cagttaattt tacgccgtac
    12121 ttatgagcaa aattttctgc ttcttttaga tatgaatttt caaaatcacc tgtttgccaa
    12181 tagttatatg cagcggtagt caaataagac gtagcatctg aataagtggc tggtaaaaca
    12241 gtatttgatg tattttcatt gattttattc aatagttcat agtatttttg tcctccttag
    12301 tagtcaaact ttttctcagc tgcttctgtt tgataggcca atgagttaga tgtagccacg
    12361 cccagctcca gtgcgttggc taagcattct tcatctgtca gcataaatgt aagaaattgt
    12421 tgtgccactt gtggattttt ggcatcttta gggacataaa aacttgatcc tcctgatact
    12481 gcaccttttc ctggattttc ttttgaaaaa ggaatcagtg gagccatgcg ccatttgtca
    12541 gatgcatcag gaaaattcaa agacatattc gtagcatacc agccgccagc aacaaacatt
    12601 gcagattctt gaaatgcttt ctcagcatct tgtgcaccgt agaaatgtac aatatctgag
    12661 tcgatcattt ctttgattaa ctttgcagca tgtttcgctt ctttagtatt cagacggata
    12721 tttccttctt catcaagcaa agattgattc tgctgggcta gcatgttgat aaacaattct
    12781 tggtccccag attctggcaa ggcaattcca aaactaccag ttttttcttt taatgctttt
    12841 gccacctgga tatattcttc ccaagaactg atttcttcaa aatctacacc tatttcagca
    12901 aatttttttg gttgataata acaaagtgct ggggtaaaat cactagccca aggaataatc
    12961 gtttcattac tcgtttgttg acgcaaaata ttcaattttt gatcataaaa ctgatcgcca
    13021 tatttgtcat aaaatccata ttcatcagca ctataaaaag catcttgaaa tttttccagc
    13081 acacctgctg ctgaagtgtc tgccatggag ataatatccg gcatatcttc gtgtgttacc
    13141 aattttgggg tgatcatttg tactgttgct gttcccgaag ctacattcac aatttttaag
    13201 tcaatatttg ctccagtttt gcggttatag atttttgcag cattttcaac atagtttgtc
    13261 agtttgccgt ttgcgacata tacttctaat gtagtggctt tttttatcat tggatctgcg
    13321 atgtttgctg ttacttcttc gttagttttt ccacatgcac ttaagctgaa taatagggta
    13381 atgtttagca aagctatcac caatttgtat ctttttttca tgtctatccc ttctttcttt
    13441 tcaagttctt atataaaacg ctttcactta tgtatattaa cggaattagt aacacaaata
    13501 tttatataaa aaaatatttt acttgtcgtt ttggcttttc atttttgtta taatagtcat
    13561 taattataaa tagaaagctt tttatataaa aagagaaagg aaagtggttc ttttgttaac
    13621 aaacaaccaa tcctccaatc ttgtcaaaaa aacactaacc tattccatag atacccatct
    13681 tcatttatct gctgctccct attttcgact actaataaat gaaagcgcta atttatatgc
    13741 tgaatttgca cataaatcta tagaactaga taatccttcc cttttattca ttgataaaca
    13801 gcaagaagtc caaatctttt ctactcaaaa ggaaacacaa acttttatca ttatccagtt
    13861 ttattttgat gagcaattat tagcacatat tacgggacaa tctttttcta tagatcaaga
    13921 aataacatct atcattactt ttctgaaaaa tgaacagttc aaaattgatt atctaaaaca
    13981 tagtaagcca gctatttcct atgatcagga aatcaattct tttttcatac tatgccaaca
    14041 gatgagcatt catttgcttt ccttattagt tacttattgt ctgcaaaaaa ttcaattgtc
    14101 ttccttacca aggcattatc aacaggaact tgctttattg acaatacctt caaatgagaa
    14161 agtcattcct aaaaaaaagc atattcttta ttcagatgaa ttagttgatc caatcatacg
    14221 atatttagaa gaacatcttt cagaaaattt tttgcttgaa caattagccc aaattttttt
    14281 tatcagtcca gccagcttaa aaaaacattt tagaaaagtg aatggaaaaa gtgttattag
    14341 ctattttaaa gaactaaaaa tggaaaaagc aaaacgtctc gtgctagaaa atgaattaac
    14401 ctatacagaa attgcagata tattaggatt tcattcgatc catcattttt cttcagcttt
    14461 taaaaaatat acagggttat ccccttcaaa gtattatcta tccttaaaat agaaaaaaat
    14521 ttttaggttt tatccacaaa ttatttctct atattttctg tctattcaaa ataaacaata
    14581 attttccggc acaattattt gctgtttttt accataacat agtacgatca acgtagaaaa
    14641 ggagatgttt ttatgaaagt tgttgtcatt ggcggtgtag ctggcggtcc ttcatttgcc
    14701 actcgtttcc gtcgattgaa tgaagcgcac gaaatcatta tctatgaacg cggagaaaat
    14761 atttcttatg caagttgtgc tttgccttat tatttaggag gtgtgatcac tgaccgtgac
    14821 tcgctgatcg aacgtacacc agaaatattg aaaacaaaaa acaacatcga tgtctttact
    14881 aaacacgaag taacagcaat cgatccttct actaagcgat taacagttaa agacctatcc
    14941 acaaatgaag aaataaaaac agattacgat aagttgatca tctcttctgg tgctagacca
    15001 gattatccgg atattcccgg agtttttgaa gcagaaaacg gctttgtcct ccgtagtgtg
    15061 acggatgcgg atcgaatcaa atcgttcctt gaagaaaaaa atccacaaca tgtcgtcatt
    15121 cttggtgcag gtgttatggg tctggaatta gctgagaatt tcaagcatcg cggcttagac
    15181 gtgactttaa tcgatcaatt gccacaagtc gctttccctt atgatccaga aattgctaat
    15241 ttagtttatg acaaattgct gaaagaagga ttagccgttc atttagaaac aagagttact
    15301 gagatccgtg ataaaggtcg agaaatagta ttatcagatg gctccgtcct ttctgctgat
    15361 atgctaattt ttgctgttgg tgtttctccg aagaatgaag tggtgaaagc aaccggcata
    15421 caattatcag atacaggtca gatcattgtt gatgaccagt tacaaaccaa tctgccggac
    15481 atctatgcga ttggcgatat tatcgaaacg actagtgtag tgactggtca gccgatccaa
    15541 agtatgcttt ccagtgcggc caatcgtcaa ggacatatgc tggcagatat tttaaatggt
    15601 acgcctatgc gctatcgcgg atatattggt gcaggtgtcg caaaaatctt tgatcataca
    15661 gcaagttatg cgggaatgac agaacatgaa ttaaaagcat caggcataac aaattataaa
    15721 actgttttta tcactccctt tgaccatgcc tatttctatc cagaagctac aagattaaat
    15781 ctaaagctga tttttgatgc agatagcggt cgtattttag gtggacaagc atttggagaa
    15841 aaaggtgtcg ataaacggat gggagaactt tctgtagcaa tcaccggaaa cttgacagtc
    15901 tttgatttgc ctgatttgga gttgccttac tctccaccgt attctactac ccgtgacccg
    15961 ttgaatatag ctggttatgt cgcaatcaat caaatgacga atatcgtaga aacgatcaaa
    16021 gcaagtgata tacccgaaaa cgatttgaaa gaagcgttct ttttagatat acgtgaacct
    16081 aataaagcac cttctggcag tatctcggca acaaaaaata ttccaatgaa tgagttacga
    16141 gatcgaatca atgaaatccc aaaagataaa aaagtttaca ttactttcag aagaggattg
    16201 aatacttata cttctgcccg aatcttggca ggtttgggta tcaaagcgat tttgattgaa
    16261 gaataaaaaa acgaccgcgg tgggtagccg cggtctagga gttattttta cttgaggagt
    16321 aaaaatgaaa aagtatatag ggttgttggt tggtatgtat atagaataga agataaatgt
    16381 gaagaaattg tgcattattt cttcacattt attaggttat agtttaagaa agtttaaaca
    16441 ttgcgcaatt taattatttt agctgtttct tttcataaaa aaacatgttg tgactctgag
    16501 acaaatcata gtccatagtg atgtctctag agtaactacg cctaatatta cgaggcatca
    16561 tattaatcta tcagcactag tacctaaact agcatccctt tcgaatattt tttcttatat
    16621 aataaagttt gctttcactc ctctaatctc accaaatcga ctggcttgcg ttgtgtgata
    16681 ctag
//
LOCUS       DACDKM010000058        16573 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20834_2016
            SAMN12640370-rid9891663.denovo.058, whole genome shotgun sequence.
ACCESSION   DACDKM010000058 DACDKM010000000
VERSION     DACDKM010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640370
            Sequence Read Archive: SRR10040891
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16573)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16573)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742835.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742835.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:04:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,012
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 29 of 75
            Pseudo Genes (incomplete)         :: 33 of 75
            Pseudo Genes (internal stop)      :: 19 of 75
            Pseudo Genes (multiple problems)  :: 5 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16573
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640370-rid9891663.denovo.058"
                     /isolate="E20834_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            297..1406
                     /locus_tag="IV334_001709"
     CDS             297..1406
                     /locus_tag="IV334_001709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HAP8943780.1"
                     /translation="MLDEFEKQNPDIKVEMETTPWDQYWVKLEAATTGGNMPDVVTMH
                     SSESYKYMSQNALMGLDDVIKENDIDMGNFTEGIADFYTLEDELYAIPKDASVVGLWY
                     NKELFDNAGVDYPDETWTWETLRQAAIDLTDESKGIYGFASHIGTETGYWPFIYQNGG
                     EVFADDNTKSGLNTPEAQEALAFYRDIALVDHASPTITELQDPDKLSRFQSGRLAMMI
                     EGNWFAPGFLKNDYLVENVASAVLPKGKVNATITNGLGWAASASTKYPEEVKKLMAFL
                     ASEDANKIQAETGASIPALKGFEDEWAASSDTIDLSAFGKMLEYGVPRPYNKDGLRAE
                     QAEVQIINRILSGEVTVEDGTAQIEKEVNAILSGN"
     gene            1489..2385
                     /locus_tag="IV334_001710"
                     /pseudo
     CDS             1489..2385
                     /locus_tag="IV334_001710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008378728.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
     gene            2401..3225
                     /locus_tag="IV334_001711"
     CDS             2401..3225
                     /locus_tag="IV334_001711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002579255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAP8943781.1"
                     /translation="MKNKFFSNAPIHIMLILGGIAMALPFIWMILTSGKTLVESTQIP
                     PTIFPEKFQLSNYQQVLDLLPFMAFYINTFLMVVGRVIGSVLFSAMAAYACARLSFPG
                     KNILFSLVLIQMMIPGSIFITPQYLLAQKLGILNTIPALILPGVVSAFGTFLLRQFFL
                     GLPDELEEAAYLEGANIWTIFFKIMLPLAKSGLVALGIFTALFAFKDLMWPLIVNTSI
                     DKMPLAPGLANLQGQYATNFPQLMAGSLLAIWPIVVIFIVFQRKFIEGIATTGSKL"
     gene            3257..4567
                     /locus_tag="IV334_001712"
     CDS             3257..4567
                     /locus_tag="IV334_001712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005235059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase/alpha-galactosidase"
                     /protein_id="HAP8943782.1"
                     /translation="MTKITFIGAGSTIFAKNVLGDAMLTPSLQDAHIALYDIDEKRLK
                     ESNLMLKNINKNSNENRADIVSYYDRKEALREADFVINAIQVGGYKPSTVIDFDIPKK
                     YGLRQTIGDTMGIGGIFRTLRTLPVMLDFAKEMEEVCPNAWLLNYTNPMAMLTMGVLK
                     ATSIKTVGLCHSVQVCVPELFEHLGIADQYDLNDFQWKIAGINHMAFLLEITKNGEDF
                     YPTIRQLAAEKPNPHPDSVRFELLKRFGYYITESSEHNAEYHPYFIKSKYPELIDELG
                     IPLDEYLRRCEQQINDWESMREDIVNNGELTHTRSREYASYIMDSITTGTPSVIAGNV
                     LNKGLITNLPTDVCVEVPCLVDKNGVQPTYVGELPTQLAALNRTNINVQELTVEAALT
                     LDKSKIYQAAYLDPHLSSELSLTDIKNLVDDLIEAHGDYLPEYN"
     gene            4715..5974
                     /locus_tag="IV334_001713"
     CDS             4715..5974
                     /locus_tag="IV334_001713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HAP8943783.1"
                     /translation="MKWSKKIILACIALGATISLTACGKNQEDASGKVEIEFFNQKKE
                     MTQTIQEIAKDFEAKNPDIHVKVVDVPNAGEVIKTRMLAGDVPDVINLYPQSIELKEW
                     AKAGYLEDLTEEPYLENIKNGYAQRFAIEDRVYSIPLTANVYGFYYNKTAFEEMGIQA
                     PETWAEFEKIVAEIKDQNKVPFAIAGAEGWTLNGYHQLAIATAAGGEEEANNVWRFSE
                     VNGINAESKEMQKDFNRLDLLREPKALQNNWQGAGYNDTVVTFTKGDALIMPNGSWAM
                     PMIHSQNPDFEVGTFPFPADEAGQSLTIGAGDLALSVSATSQHKEEAKRFVEYMTTPE
                     AMQKYYDVDGAPCAVEGVIENTQDSPLSGLTELAFTDRHLVWLAKDWNSENDFYTLTT
                     NYLHNGNQKAMINALNAFFNPMKADGR"
     gene            5984..6856
                     /locus_tag="IV334_001714"
     CDS             5984..6856
                     /locus_tag="IV334_001714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018363968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP8943784.1"
                     /translation="MKIKEILNRYWAIIFVAIPIILQMIFFYFPMVQGAFYSLTNWTG
                     LTYNFDFVGLNNYKILFSDPKFIKSIGFTLIVTLCMIAGQIGFGILIARALNANIKGK
                     TFFRAWFFFPAVLSGVVVSLVFKQIFNYGLPKIGSMLGIEFLQTSLLGTTWGAIFAVI
                     FVLLWQGIAMPVIIFLAGFQSIPQEIIEAARMDGANDKQIFWNIEITYLLPSISMVFI
                     MALKGGLTAFDQIFALTGGGPSDATTSLGLLVYNYAFKNNQFGYANAIALVLFILIGI
                     VSLAQLKLSKKFEI"
     gene            6868..7698
                     /locus_tag="IV334_001715"
     CDS             6868..7698
                     /locus_tag="IV334_001715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000711263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAP8943785.1"
                     /translation="MKTSKEKNISKYLLLLLGGFMILVPLVSTLFGSFKTTPDIMNNF
                     FRFPNPATLENFRRLLADGIGHYFWNSAIITIIAVVAVTAIIPMAAYSLARKMTRGKA
                     YGLMYLFLILGIFVPFQVIMIPITVMMSKIGLANMWGLILLYLTYAVPQTLFLYVGFI
                     KLNVPISLDEAAEIDGASHFTTYFKIVFPMLKPMHATTIILNALWFWNDFMLPLLILN
                     RDSNMWTLPLFQYNYTGQYFNDYGPSFASYTVGIVTITLVYLAFQRSIIAGMSNGAVK
                     "
     gene            7738..9921
                     /locus_tag="IV334_001716"
     CDS             7738..9921
                     /locus_tag="IV334_001716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HAP8943786.1"
                     /translation="MIIFDNHTQTFHLQNKKISYLLGIEENNYLTHLYFGKKITHYSG
                     GYRYPRTDRSFSPNNANAQDRLLSLDTLPLEFPGYGHGDFREPAHNIKLGNGSRVNDF
                     RYDSYEIQKGKPSLAGLPALYTEDDDEAETLIIQLKDAISGLTLQLFYTIYQNHSVII
                     RSTQLVNNSDETVEINRLASQSIDFPNRPLELIHLNGTWARERQMTREKIHTGIKVLD
                     SKRGSSSHQQNPFVALVDAKTDEFQGEAYGFNLVYSGNHETVIQKDPFDQTRVIMGIN
                     SFNFGWQLKTGDSFQTPEVVMVYSNEGLNTLSQTYHELYNHRLIRGNYRLKERPTLIN
                     NWEATYFDFNEEKIMNIVDDAKNLGIEMFVLDDGWFGGRENDFTSLGDWVETEGKLAC
                     GLENLAKQVHDNGMKFGIWFEPEMISEDSELFRAHPDWALGIPGRDRSISRNQYVLDF
                     SRQDVRDNIYQQMTAIFDRVPIDYVKWDMNRNMTEVYSVLLDSTMQGEVAHRYMLGLY
                     EFLEKLTTNYPTILFESCSGGGGRFDAGFLYYMPQTWTSDNTDAVARLKIQYGTSLVY
                     PISSMGAHVSAVPNHQTGRETSLTIRGNAAMAGVFGYELDLTQLTSEEKAEIKEQVAF
                     YKKHRQLLQYGTFYRLESPFEGNDTAWMFVSKDQTEAMVFYFRVLAEAAHPLVTLKLA
                     GLAEEATYQMEDVTFAGDELMNLGFYIDSELHGDYATQRFYLKQI"
     gene            9982..11202
                     /locus_tag="IV334_001717"
     CDS             9982..11202
                     /locus_tag="IV334_001717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligo-1,6-glucosidase"
                     /protein_id="HAP8943787.1"
                     /translation="MFYLHAFTKKQPDLNWENEEVREEIYAMINYWLDKGLGGFRIDA
                     ILNIKKKLEYGIFESDGEDGLAFIGPWILNQSGIEVWLKEMRERTFKPHNSMTVAEAD
                     VPNERLDEYIGEDGYYSMVFDFSYTDIDVPETGEWFKDSQWTWTDMRTNIFTNQLVTQ
                     DKGWGALYLENHDQPRSINKYIPEEWINDYSKKMLATLFMLLRGTPFIYQGQELGMTN
                     IEMASLEDFDDVATHSQYKRALEYGLAPEQALKAVNKRSRDNSRTPMQWNTQKNARFS
                     TSDNVWLKVNPNYPELNAEAQLTNQQSVFNYYHQLIKLRKNSVYKDVLIYGQFLPVKD
                     KNEHILLYERQLEDKIVLVILNVTATEQEYYVDEQYQQVLINNYEDVLLDQSRKLRLR
                     PFESIVLANYGGQL"
     gene            11199..12659
                     /gene="gtfA"
                     /locus_tag="IV334_001718"
     CDS             11199..12659
                     /gene="gtfA"
                     /locus_tag="IV334_001718"
                     /EC_number="2.4.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose phosphorylase"
                     /protein_id="HAP8943788.1"
                     /translation="MKIKNEAMLITYADSLGKNMGELKQVLDKHLQGVIGGVHLLPFF
                     PSTGDRGFAPSDYTTVDPALGTWEDVEALGEDYYLMFDFMINHISRESKFFQDFKKNH
                     EQSPYKEMFIRIHEFFPENRPTQEDIDLIYKRKDKAPFQTVEFADGTTEEVWNTFGEE
                     QIDLDVTKEVVKEFIRETIKDMANHGCSLIRLDAFAYAIKKLDTNDFFVEPDIWELLD
                     EVRQEAAKYDVELLPEIHEHYSIQMKIANHDYYIYDFALPMVTLYSLYSGKSERLANW
                     LKMSPMKQFTTLDTHDGIGVVDARDLLTDEELDYTSEELYKVGANVKKVYSSANYNNL
                     DIYQINSTYYSALGDNDRSYLLARAIQVFAPGIPQIYYVGLLAGKNDIELLEATKEGR
                     NINRHYYDLAEIEEEVQRPVIQQLFALLKFRNESAAFDLDGTIEVATPSESEIVITRK
                     NKNGSDSATLTANLDTKEFIIESKDIKIFTSVALKF"
     gene            12736..13842
                     /locus_tag="IV334_001719"
                     /pseudo
     CDS             12736..13842
                     /locus_tag="IV334_001719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175341.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
     gene            14164..14373
                     /locus_tag="IV334_001720"
     CDS             14164..14373
                     /locus_tag="IV334_001720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 domain-containing protein"
                     /protein_id="HAP8943789.1"
                     /translation="MVVITKNELMKLGYGKYQSERIIRQAKAIMVSKSYTYYENRRLG
                     LVPVESVEEVLGMKIEVEEDFQNHG"
     gene            14366..14515
                     /locus_tag="IV334_001721"
     CDS             14366..14515
                     /locus_tag="IV334_001721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HAP8943790.1"
                     /translation="MVNFKKDKNGKYHFTASLGFDKRTGKRVQKNVEGLKQLKKKKSV
                     YRTCF"
     gene            14550..15334
                     /locus_tag="IV334_001722"
                     /pseudo
     CDS             14550..15334
                     /locus_tag="IV334_001722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922412.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
     gene            complement(15618..>16573)
                     /gene="xerS"
                     /locus_tag="IV334_001723"
     CDS             complement(15618..>16573)
                     /gene="xerS"
                     /locus_tag="IV334_001723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tyrosine recombinase XerS"
                     /protein_id="HAP8943791.1"
                     /translation="YKLFYEWLLSESIVPDTSITSIRQLTAYDLNLYKSHLKRRTKEN
                     VKKETTKTKLKDNNNLGLSTSTINRNITALKVLFKYLSKSSNNPLGKPYLDNNPMDQV
                     ATITDKTTLAARANAIEKKLFLDKDTQNYLDYIANEYKNTLSKRALIYYQRDVERDLA
                     INALILGSGLRLSEVININLDDLSLDKNNVVVTRKGNKRDAVNIAAFAMEYLANYLAI
                     RKERYKVTENEKALFLAIYQGEAKRISGIAIERMVAKYSKGFRIQVSPHKLRHTLATR
                     LYQQTNSLVLTAQQLGHSSTNTTTLYTHIDNAATIDALNAL"
BASE COUNT         5612 a         2518 c         3213 g         5230 t
ORIGIN      
        1 taaaaaagta aacatccaaa aaagggtgtt tacttttttg tttactaata tttttagtaa
       61 aaaatctcga caaaacatta agtaaacagt tatggtgttg ttaacaaaaa gaaaacgcat
      121 tcaaaacagg aggaattaag atgaaacatt tcaaaaaaat agctttaaca gggatagctg
      181 ttttaactgg gacattactc gcagcatgtg gtgggggaga tagtaattct aaagacgggc
      241 aagtaacttt aagctatgcg agttgagata ctgaccaagc tccaggacta agaaaaatgt
      301 tagatgaatt tgaaaaacaa aatcctgata tcaaagtaga aatggagaca acgccttggg
      361 atcaatattg ggttaaatta gaagctgcaa caacgggagg taatatgcca gatgtagtaa
      421 ccatgcactc tagcgaatcc tataaatata tgtcacagaa tgctttgatg ggacttgatg
      481 atgttatcaa agaaaatgat attgacatgg gaaactttac tgaaggaatt gctgatttct
      541 atacgcttga agacgaacta tacgcaatcc ctaaagatgc ttctgtagtg gggctatggt
      601 acaacaaaga attatttgat aatgcaggtg tcgattatcc agatgaaaca tggacatggg
      661 aaacactcag acaagctgca attgatttaa cagatgaatc aaaaggcatc tatggttttg
      721 catcacatat tggaacagag actggctact ggccatttat ttatcaaaat gggggagaag
      781 tatttgccga tgacaataca aaatcaggtt tgaatacccc agaagcacaa gaagctttag
      841 ctttttatcg agatattgca cttgttgatc atgcttcacc aacaattaca gaattacaag
      901 atcctgataa gttaagtcgt tttcaatcag gtagattagc aatgatgatt gaaggaaact
      961 ggtttgcacc gggatttttg aaaaatgact atctagttga aaatgttgct tctgccgtgt
     1021 tacctaaggg aaaagttaat gcaacaatta caaatggact tggttgggct gccagtgcga
     1081 gtacaaaata cccagaagaa gtgaaaaaac taatggcatt tttagctagt gaggacgcca
     1141 ataaaatcca agcggaaacg ggggcatcaa tcccagcact aaaaggcttt gaagacgagt
     1201 gggctgcttc ttcagataca attgatttga gtgcttttgg aaaaatgttg gagtatggag
     1261 tccctcgtcc atacaataaa gatggattac gagctgaaca agcagaagtt caaattatta
     1321 accgtattct gtctggtgaa gtaactgttg aagatggaac cgctcaaatt gaaaaagaag
     1381 tcaatgcgat attgagcgga aattaaatta gttaattata caatatacag gcgaaaggat
     1441 tctatttcgt ctgtatatta atggatgaat accaaaaagg gggatattat gaaaaatcga
     1501 atttttggaa aatatcgtaa agatcaagtg atatggggct atatttttat ttttccaacg
     1561 gcattaggat taattttatt aaatatatgg ccagcaattc aaacagcaat tttaagtttt
     1621 caaaaaacgg tagggttcgg tgatactgaa tgggtcggat taagaaatta ccaaaaaatg
     1681 tttgcagacg gtgaagtttt tcagtcttta ttaaacacat taatttatgc agtggtttcg
     1741 atacctagta ttgttgtatt atccttgttg gtggcagttt taatgaatag aaaagttaaa
     1801 ggattgtcga tttatcggac aatttatttt ctaccagtcg ttgctgcacc atccgcagtc
     1861 gcaatgattt ggcgctggat gttcaataat gattatggac tgattaataa catgttggct
     1921 aaaataggtc tcgatggacc agcatggtta acggatccat cgattgcgat ttattctatt
     1981 atcattgtag gagtttggag tgctattggt tataacatgg tgttattact agccggctta
     2041 taagaaattc ctaaagatta ttatgaagca gcggaaattg atggtgctag tccaatccag
     2101 cagtttttca acattacgtt accattagtt acaccaacat tattttttgt agttgtaaca
     2161 acagttatca atgctttcca agtatttgat gttattttta tgatgattac tcctaatagt
     2221 accgccatgt ttaaatcaca atcattagcc tatttattct ataaacattc atttatttta
     2281 aatgacaagg gatatggttc ggcaatcgtt atgttccttt tagtgattat attaattatt
     2341 actgcaattc aaatgaagtt gcagaaaaag tgggtaaact attaaggagg ctatcaaatt
     2401 atgaaaaata aatttttttc aaatgcgcca atccatatca tgcttatttt aggtggtatt
     2461 gcaatggcat taccttttat ttggatgatt ttaacttcag ggaaaacgtt ggttgaatct
     2521 acccaaattc cacctacaat tttccctgag aaatttcagc taagtaacta tcaacaagta
     2581 cttgatttat taccattcat ggctttttat atcaatacat ttttaatggt tgttggacga
     2641 gtgattggct cggttttatt ttcagcaatg gctgcatatg cttgtgcgcg tctaagtttt
     2701 ccagggaaaa atatcctttt cagcttagta cttattcaaa tgatgattcc cggttccatt
     2761 tttattactc cacagtattt attagcacaa aaattgggta ttttaaatac aattccagca
     2821 ttaatattac caggggtagt tagtgcgttt ggtacgtttt tattaagaca atttttctta
     2881 ggtttacctg atgaattgga ggaagcggcc tatttagaag gtgccaatat ttggacgatc
     2941 tttttcaaaa ttatgttgcc gcttgctaaa tcaggattgg ttgcacttgg aatattcaca
     3001 gcgttgtttg catttaaaga tttaatgtgg ccattgattg tcaatacttc aattgataag
     3061 atgcctttag ctccgggatt agcaaactta caaggacaat atgccacgaa cttcccacaa
     3121 ttaatggcag gttcgttatt agcaatttgg ccaatagttg tgatattcat cgttttccaa
     3181 agaaaattca ttgaaggaat tgctacaaca ggtagtaaat tataaaacta ttaattagtg
     3241 attggagagt aagtacatga caaaaattac atttattggt gcaggaagta ctatttttgc
     3301 aaaaaacgta ttaggcgatg cgatgttaac tccaagtcta caagatgcac atattgcatt
     3361 gtacgatatt gatgaaaaac gactaaaaga atcaaattta atgcttaaaa atattaataa
     3421 aaattcgaat gaaaatcgtg ctgatattgt ttcttattat gatcgcaaag aagcgttaag
     3481 agaggctgat tttgttatca atgctattca agtgggtggt tataaacctt caacagtgat
     3541 tgattttgat attcctaaaa aatatgggtt gcgacaaact attggtgaca ccatggggat
     3601 tggtgggatt tttagaaccc tacggacact accagtaatg ttagactttg ctaaagaaat
     3661 ggaagaagtt tgtccaaatg cttggttatt gaactataca aatccgatgg cgatgttaac
     3721 catgggtgtt ttaaaagcga cgagtattaa gactgttggt ttatgtcata gcgttcaagt
     3781 atgcgtacca gaattgtttg agcatttagg aatagccgat caatatgact taaatgactt
     3841 tcaatggaaa attgcaggca ttaatcatat ggcattcttg ttggaaatta caaaaaatgg
     3901 ggaagatttc tatccaacaa ttcgtcagtt agctgcagaa aaaccaaatc cacatccaga
     3961 ttctgtacgt tttgaattgt tgaaacgttt tggttactat attacagaat caagtgaaca
     4021 caatgcagaa taccatccat actttattaa atcaaaatac ccagaattga ttgacgaatt
     4081 agggattccg ttggatgaat acttacgtcg ttgtgaacaa caaattaatg attgggaaag
     4141 catgcgagag gacattgtaa ataatgggga gttaacacat acgagaagcc gtgaatatgc
     4201 ttcatatatc atggattcta ttacgacagg aactccgtca gtgattgcag gaaatgtctt
     4261 aaataaaggc ttaatcacaa acttaccgac agatgtttgt gtggaagtac cttgtttagt
     4321 cgataaaaat ggcgtgcaac caacctatgt cggtgaattg ccaacacaat tagctgcttt
     4381 aaatcggacg aatatcaatg ttcaagaact gacagtagaa gccgctttaa cactagataa
     4441 atcaaaaatt tatcaagcag cgtatttaga tccacatcta tcatctgaat tgtctttaac
     4501 agatatcaaa aatttagtgg atgatttaat cgaagcacat ggggactatt tacctgaata
     4561 taattaaaaa gaagctgaaa gagtaaggaa ttttcaattc cttactcttt tcttagttaa
     4621 atttagtaaa aatattgaca aaacacttag taaacagtta cattagtgtt aacaaaaaga
     4681 aaacgcattc aaattgtata aggaggagca taaaatgaag tggtctaaaa aaattatttt
     4741 agcttgtata gcactaggag caacgatctc tttgactgct tgtggaaaaa atcaggaaga
     4801 tgcaagcggt aaggtagaaa tagagttttt taatcaaaaa aaagaaatga ctcaaacgat
     4861 tcaggagatt gctaaagatt ttgaagcaaa aaaccctgat attcatgtaa aagttgtgga
     4921 tgtaccgaat gctggtgaag taattaaaac acgtatgctt gcaggcgatg taccagatgt
     4981 cattaatctt tatcctcaaa gtatagaatt gaaagaatgg gcgaaagctg gttatttaga
     5041 ggacttaacc gaagaaccgt atttagagaa tatcaaaaat ggatatgcac aacgttttgc
     5101 tattgaggat cgtgtatata gcataccatt aaccgctaat gtctatggtt tttactataa
     5161 caaaactgct tttgaggaaa tggggattca agcaccagaa acttgggcag aatttgaaaa
     5221 aatagtagct gagattaaag atcaaaacaa agtaccgttt gcgattgcag gagcagaagg
     5281 ctggacttta aatggttatc accaactagc tatagcgact gctgctggtg gtgaagagga
     5341 agcgaacaat gtttggcgtt tctcagaagt taatggaatt aatgctgaaa gcaaagaaat
     5401 gcaaaaagat tttaatcgat tagatttgtt aagagaacca aaagctttgc aaaacaactg
     5461 gcaaggtgcc ggttataacg atacagtagt tacttttaca aaaggtgatg cgttgattat
     5521 gccgaatggt tcatgggcaa tgccgatgat tcattcacaa aatccagatt ttgaagtagg
     5581 aacttttcct tttccagcag atgaagcagg tcaaagtcta acgattggcg caggagacct
     5641 agccttatca gtttctgcaa cttctcaaca taaagaagaa gccaaacgtt ttgtagaata
     5701 tatgacaaca cctgaagcga tgcagaaata ttatgatgta gatggtgcac cgtgtgctgt
     5761 tgagggcgtt atcgagaata ctcaggattc accattaagt ggtttaactg aacttgcttt
     5821 tactgatcgt catttagttt ggttagccaa agattggaat agtgaaaatg atttttatac
     5881 cttaacaaca aattatttac acaatggcaa tcaaaaagcc atgattaacg cattaaatgc
     5941 tttctttaat ccaatgaaag ctgacggaag gtaggaaaat aaaatgaaaa taaaagaaat
     6001 tttaaatcgt tactgggcaa tcatttttgt agcaattcca attattttgc agatgatttt
     6061 tttctacttt ccaatggttc aaggtgcttt ttacagttta actaattgga ctggtctgac
     6121 ttataatttt gattttgtgg gtttgaacaa ttataagatt cttttttctg atccgaagtt
     6181 tatcaaatcg attggcttca ctttaattgt gacgctttgc atgattgctg gacaaattgg
     6241 ttttggtatt ttgattgcgc gggcgttgaa cgcaaatatt aaagggaaaa cattctttag
     6301 agcatggttc ttttttccag cggtcttatc gggcgtggtt gtttctttag tatttaaaca
     6361 aatttttaac tatggtttac caaaaattgg tagtatgtta ggcattgaat ttttacaaac
     6421 cagtctttta ggaacaacat ggggagcgat ttttgcagta atttttgttt tactttggca
     6481 aggaattgcc atgccagtca tcattttctt agcgggtttt caaagtattc cacaagaaat
     6541 tattgaagct gctagaatgg atggggcgaa tgataagcaa attttttgga atattgaaat
     6601 cacctactta ttaccaagta tttcaatggt atttattatg gcattaaaag gtggcttaac
     6661 agcatttgat caaatttttg ctttaactgg tggtggacca agcgatgcca caacatcttt
     6721 ggggttactt gtttataatt atgcttttaa aaacaatcag tttggttatg cgaatgctat
     6781 tgctttagtc ttatttatct taattgggat tgtttcactg gcacaattaa aattatcgaa
     6841 aaaatttgaa atctaggagg tttcccaatg aaaacatcaa aagaaaaaaa tatttctaaa
     6901 tacttgcttt tattattagg gggcttcatg attttagttc ccttagtttc aacgctattc
     6961 ggttcattta aaacaacacc cgatattatg aataatttct ttcgttttcc taatccagcg
     7021 actttggaaa atttccgaag attgttagcg gatggaattg gtcattactt ttggaattcg
     7081 gcaatcatca caatcattgc agtagttgct gtgacagcaa ttattcctat ggctgcttat
     7141 tcactagcaa gaaaaatgac tcgtgggaaa gcatacggat tgatgtattt atttttaatt
     7201 ttaggaattt ttgttccttt ccaagtaatt atgattccga ttaccgttat gatgagtaaa
     7261 attggtttag caaatatgtg gggattaatc ttactctatc tcacttatgc cgtaccacag
     7321 acactatttt tatatgttgg ttttattaaa ctaaatgtgc ctatcagtct ggatgaggct
     7381 gctgaaattg atggggctag tcactttaca acttatttca aaattgtttt cccaatgtta
     7441 aaaccaatgc atgcgacgac tattattctt aatgctctat ggttttggaa tgatttcatg
     7501 ctaccattat taattttaaa tcgtgattct aatatgtgga cactaccatt gtttcaatat
     7561 aattatacgg gacaatattt taacgactat ggtccaagtt ttgcttccta tacagtagga
     7621 attgtcacga ttacactagt ttatcttgct ttccaaagaa gtattatagc tggaatgagc
     7681 aatggagcag tgaaataacc aataaattat tctcattata aaggagttca taaacttatg
     7741 attatatttg acaatcacac acaaacattt catttacaaa ataaaaaaat tagctatcta
     7801 ttaggaatcg aagaaaataa ttatttaacg catctttatt ttggaaaaaa gattacgcac
     7861 tattctggtg ggtatcgcta cccccgaaca gatcgttcct tctcacccaa caatgcaaat
     7921 gcgcaagatc gtttattatc tttagacaca ttaccattag aatttccagg atatggacat
     7981 ggtgatttta gagaaccggc gcataatatt aaattaggaa acggtagtcg tgtaaatgat
     8041 tttcgttatg atagctacga aattcaaaaa ggaaagccca gcttagcggg attgccagcc
     8101 ttatatacgg aagatgatga tgaagctgaa acgctgatta ttcaattgaa agacgcgatt
     8161 agtggattga ctttacagtt gttctacaca atttatcaaa atcacagtgt aattattcgt
     8221 tctacgcaat tagtaaataa tagtgatgaa acagtggaga ttaaccgctt agctagtcaa
     8281 tcaattgatt ttccaaatcg tccactcgag ttaattcatt taaatggtac ctgggcaaga
     8341 gaacgtcaaa tgactagaga aaaaattcat acagggataa aagtattaga tagtaaacgc
     8401 ggatcaagca gccatcaaca aaatccgttt gttgctttag tagatgccaa aacagatgaa
     8461 tttcaaggag aggcatatgg atttaattta gtttatagcg gtaatcacga aacggtgatt
     8521 caaaaagatc catttgacca aacacgggtc attatgggaa ttaattcatt taattttggt
     8581 tggcaactaa aaactggcga ctcatttcaa acgccagaag tcgtgatggt ttatagtaat
     8641 gaaggtctga atacgttatc acagacgtat catgaattgt ataatcaccg tttaattcgt
     8701 ggaaactatc gtttaaaaga acgtcccacg ttaatcaaca actgggaagc aacgtatttt
     8761 gattttaacg aagaaaaaat catgaatatt gtcgatgatg cgaagaactt gggcattgaa
     8821 atgtttgttt tagatgatgg ttggtttggc ggtcgtgaaa atgacttcac ttcattaggt
     8881 gattgggttg aaacagaagg caaacttgct tgtggtttgg aaaatttagc caagcaagtg
     8941 catgataacg ggatgaaatt tgggatttgg tttgaaccgg aaatgatttc tgaagatagt
     9001 gaactatttc gagcacatcc agattgggca ttgggtattc caggtcgtga ccgctcaatt
     9061 agtcgaaatc aatatgttct tgatttttca agacaagatg ttcgtgataa tatctatcaa
     9121 caaatgacgg cgatttttga tcgtgtaccg attgattatg tgaagtggga tatgaatcga
     9181 aatatgacag aagtctattc tgtcttgttg gactcaacga tgcaaggtga agtggctcat
     9241 cgttatatgt tagggttata tgaattttta gaaaagttaa caacgaatta tccaactatt
     9301 ttatttgaaa gttgttcggg tggaggcggt cgttttgatg caggtttctt gtattacatg
     9361 ccacaaactt ggacgagcga caataccgat gccgtggctc gtctgaaaat tcaatatgga
     9421 accagcttag tgtatccaat ttcaagtatg ggtgcgcatg tttcagcagt cccaaatcat
     9481 caaacaggaa gagaaacgag tttaactatt cgtggcaatg ctgcgatggc tggtgttttt
     9541 ggttatgagc tagatctaac gcaattaact tctgaagaaa aagcagaaat taaagaacaa
     9601 gttgcctttt ataaaaagca tcgccaactt ttgcaatatg gaacttttta tcgcttggaa
     9661 agtccgtttg aaggcaatga tactgcatgg atgtttgtct caaaagacca aacagaagcc
     9721 atggtctttt atttccgagt attagcagaa gctgcgcatc cattagtcac attgaaatta
     9781 gcaggattag cagaagaagc aacttatcaa atggaagatg taacatttgc tggcgatgaa
     9841 ttgatgaatc tcggttttta tattgactca gaactacatg gtgattatgc gacacaacgc
     9901 ttttatttga aacagatata gtagcttgaa tttacaatat aagtgcaaca ccaaaagaga
     9961 ctcattccaa acgaagaaaa tatgttttat ttacacgcat ttactaaaaa gcagcctgat
    10021 ttgaattggg aaaatgaaga agtacgtgaa gaaatctatg caatgattaa ctattggtta
    10081 gataaaggac taggcggatt tagaatcgat gcgatattaa acatcaaaaa aaaactagag
    10141 tatggcatct ttgaatctga tggagaagat gggttagcct tcattgggcc ctggattctt
    10201 aatcagtcgg gcattgaagt gtggttaaaa gaaatgagag agcggacatt taaaccacat
    10261 aatagtatga cggtagcaga agccgatgtt cctaatgaac gtttagatga atatattggt
    10321 gaagatggct attattcgat ggtttttgat tttagttata cggatattga tgtacctgaa
    10381 actggtgaat ggtttaaaga tagccaatgg acatggacgg atatgcgtac caatattttt
    10441 acgaatcaat tagttacgca agataaaggt tggggcgcct tatatttaga aaaccacgat
    10501 caaccacgtt caattaataa atatattcca gaagaatgga tcaatgatta tagtaaaaaa
    10561 atgttggcga cgctttttat gttactaaga ggtacccctt ttatttatca aggacaagaa
    10621 ctaggaatga ccaacataga aatggcatca ttagaagatt ttgatgatgt agctacgcat
    10681 tctcagtata aacgggcatt agaatatgga ttggcaccag agcaagcatt gaaagctgtc
    10741 aataaacgaa gtcgggataa ttctcgcaca cctatgcaat ggaatacgca aaaaaatgcc
    10801 agattctcaa cttctgataa tgtatggtta aaagtaaatc ctaattatcc agaactcaat
    10861 gcagaagctc agttaaccaa tcaacagtcg gtattcaatt attatcatca attaattaaa
    10921 ttaagaaaaa atagtgtata caaagacgtg ctaatctacg gacaatttct accagtgaaa
    10981 gataaaaatg aacatatttt gctttatgaa cgccaattag aagataaaat cgtattagtt
    11041 atcttaaacg ttacagcaac agaacaagaa tattatgtag atgaacagta ccaacaagta
    11101 ttaatcaata attatgaaga tgttttatta gatcaaagtc gaaaattgcg tttacgaccg
    11161 tttgaaagta ttgtattagc taattatgga ggacaattat gaaaattaaa aacgaagcaa
    11221 tgctcatcac ctatgcagat agtttaggaa aaaacatggg cgaattaaaa caagtcttag
    11281 acaaacattt acaaggtgtt attggtggtg tacatctgtt gccttttttt ccatcgacag
    11341 gtgatcgagg ctttgcgccg agtgattata cgacagttga tccagctctt ggaacatggg
    11401 aagatgtgga agcattaggt gaagattatt atttgatgtt tgattttatg attaatcaca
    11461 tttctcgtga atcaaaattt ttccaagatt tcaaaaaaaa ccatgagcag tcaccataca
    11521 aagaaatgtt tatccgtatt catgaatttt tcccagaaaa tcgaccaaca caagaagata
    11581 ttgatttaat ctacaagcgt aaagacaaag cgccgtttca aacggtagaa tttgcggatg
    11641 gtacgacaga agaggtttgg aatacctttg gggaagaaca aattgattta gatgtgacca
    11701 aagaagttgt gaaagaattt attcgagaaa ccatcaaaga tatggcgaac catggctgtt
    11761 ctttaattcg attagatgcg tttgcttatg cgattaaaaa attagatacc aatgatttct
    11821 tcgtggaacc agatatttgg gaacttttag acgaagtgcg tcaagaagca gcgaaatatg
    11881 acgtcgaatt attaccagaa atccatgagc attattccat tcaaatgaaa attgccaatc
    11941 atgattatta tatctatgac ttcgccttgc caatggtcac tctgtattca ctttatagtg
    12001 gaaaatcaga acgtttagcc aattggttga aaatgagtcc gatgaagcag tttacaacat
    12061 tggatacgca tgatggaatt ggtgttgtgg acgctcgtga tttattaaca gatgaagaat
    12121 tggattacac ctctgaggaa ttgtataaag tcggggcgaa tgtgaagaag gtctattcaa
    12181 gtgccaatta caataattta gatatttatc aaattaacag cacctattat agtgcattag
    12241 gcgataatga ccgtagctat ctgttagcac gtgcgattca agtatttgcg ccagggattc
    12301 cacaaattta ttacgttggg ttattagcag gaaaaaatga tattgaactg ttggaagcaa
    12361 cgaaagaagg gcgtaatatt aatcgtcatt actatgattt agccgaaatt gaagaagaag
    12421 tgcaacggcc agtgattcaa caattatttg cacttttaaa atttagaaat gaatctgcgg
    12481 cgtttgattt ggatggaacg attgaagtag caacgccatc agaatcagaa atagtgatta
    12541 cacgtaaaaa taaaaacggt agtgatagtg caacattaac ggctaacttg gatacgaaag
    12601 aatttatcat tgagtctaaa gatataaaaa ttttcactag tgttgcatta aaattttaac
    12661 ctattccata gaacaaaaaa tcctttatac tagcacttgg atgacttatc caagaccaat
    12721 aaaaaggact catacatgga taagtataaa acaaatacag catttaataa atggttttct
    12781 gccataaaat taggaaaact ttctgtcaac tcccaaaaat gtatttttga ttacaattcg
    12841 tatcgtaaaa aactgtcttt tgaagctgtg ctcaagctct ttctctacgc agttaatgaa
    12901 gaaaaagaaa gtttgcgtga tttgagtaca gctcttatcg accgatcgct ccaactagaa
    12961 accgatgtgt catcgattag tcatacccaa ttgtctcgag catttaatgc acttgaacct
    13021 aaagtgttag aagaaatttt tcaacaactg ttagaaaaag ttcaacatca aatcaaacca
    13081 accaagcgaa atcatctgta tctgattgat tccagtacgt tttcgcttag tttaaaacgt
    13141 cataagtggg cgactttccg tcaaacaaaa tcaggtgtca agttacactt aaactattgt
    13201 tatatggacg atactacgat gtatccaact gattttacct taacaaatgc ccatgaacat
    13261 gatctcaatc aactaccagt attggtgaat caaccagagg caacttacgt gtttgatcgt
    13321 gggtattagg actttgaaaa gatggatcag atgcattggg atggctattt cttcgtcact
    13381 cggattaaaa aaaacaccaa agttcacgtg atagatactc tggaaacatc gccagaaaca
    13441 gaaattctac gagacgagct ggttcgttta ggatctaaaa cctatctaac cgccaatttc
    13501 cgactagtca ctgttcaaga taagaacgga cgtgtgtttc aatttatcac caatcgaatg
    13561 gacgtctctt caaaagaaat ctctgatatg tatcacgctc gttggcaaat cgaattgttt
    13621 ttcaagcaca tcaaacaaca catgaccatt aaaaccttct tttctcaaag tgaaaaaggc
    13681 gtgcagaatc aattgatttt aacgatgatt tccgctctcc tcacgtttct catcaaattg
    13741 gagacgaaaa cggaaaaatc agtgttccaa atcaaacgat tctttcgtta tttactgttt
    13801 cagccctttg aatgttgtct agaaaaatta attcccacat gagaaaaaag aaatttggct
    13861 cgttactttt ggttttcagt tgaataacag ataaaaaatt ttctggaaaa agtcatcctc
    13921 tgcgtcagca cagttttttt tgacttattt tcagaggaaa tagttttcag aatattctaa
    13981 aatgttttat gcaacactag tgatattttt tagaattaga tttctaatgt gcattttttg
    14041 agtattaagg agcaagtata caaagattta taaagctgat taaatttgaa taaaaatatg
    14101 caggaacgct actattttta gtagtgttct tttttatatt tgaataaaaa atgtggtggt
    14161 taaatggtgg ttataacaaa aaatgaatta atgaaattag gttatgggaa gtatcagtct
    14221 gagagaatca tacgacaggc aaaagcaatt atggtttcaa aaagctatac ctattacgaa
    14281 aatagaagat taggactagt acctgttgaa tctgtagaag aagttttagg tatgaagata
    14341 gaggtagagg aggattttca gaatcatggt taattttaaa aaagataaaa atgggaaata
    14401 tcattttact gctagtttgg gattcgacaa acgaacaggg aaaagagtac aaaaaaacgt
    14461 agaggggtta aaacagttaa agaagaaaaa aagcgtatac cgaacttgtt tctaattatg
    14521 gaaaagaagc agaaacttct caaagtacta tgttgtttga ggagtttttt tataaattat
    14581 tcctacctta ttatcaaggg aaagtagaag aaagaacata taaaaatcgg ttatctacaa
    14641 tcaaaaaaac ttttcttatt tctttaaaat ggagttaaga gaaatacgtc caattgacat
    14701 tcaaaagtgg caaaataatt tattgagcaa atataaaaat tcttatgtaa gaaacatgta
    14761 tgggattttt cagatgtcat tagatcgagc agttgttttg ggactacttg aaaaaaatcc
    14821 tgcaaaagtt attggaaatg tgaagaaaag gaaaactaca gttgattttt ggacaaaaga
    14881 agaatttaca aaagttattt caacttttta tattgatgat ttgtaccaac attttggttt
    14941 tatttgtatg tggttacttt ttatgaccgt tatgcgtttg ggagaagcca ctgcattaac
    15001 ttggaaagat attgattttg aaaataaaaa cttgcgtgtg aataaaacac tctattataa
    15061 gagagccaaa acctatgagt ttgtagaacc cgaaacggct tctagtattc gtacaatcga
    15121 actagacgac aatacaatta cctttttaaa agcttggaag gagcgtcaag tttcaatagg
    15181 aggcatgtcg tttgtacttt cttataatgg aacacctact cagaaatata caatccgtca
    15241 tattatgaga agacatgcaa cgatcgttgg tgttcatata atccgcatac acggattaag
    15301 gcatagtcat gcttctttgc ttatatcaca ataaaaagat gcacttcgaa ttaaagaaag
    15361 gttaaaacat gctgatgtac aaacgacttt aggaacttat ggtcatttgt atctaaattc
    15421 attgagagag ataacaaatg atcccacaga ttatatccaa actaattttt ctaaaatcaa
    15481 accgacacct atttacgata ataaaaaata aatgataaac aaaagtagga tcacaccaaa
    15541 agaaacacac tagaaaaact ataatacagg cttttctagc gtgttgtttc ttatttccgc
    15601 ctatttatac attatactca taaagcattt aatgcatcga tcgtagccgc attgtctata
    15661 tgggtatata aagttgttgt attggtactt gaatgtccca attgttgggc agttaatacc
    15721 aatgaatttg tctgttggta gagtctagta gctaatgtat gacgcagttt gtgagggctg
    15781 acttgaattc taaagccttt tgaatatttt gctaccatcc gttcgattgc aataccggat
    15841 atacgcttgg cttctccttg gtaaattgct agaaataaag ctttttcgtt ttctgttact
    15901 ttatatcgtt ctttccgaat agctagataa ttagctaaat attccattgc gaatgctgct
    15961 atattgacag catctctttt atttcctttt ctagttacta ccacattatt cttatctaaa
    16021 gataggtcat ctaaattaat atttataacc tcggagagtc tcaatccaga tcctaaaatc
    16081 agtgcattga tcgccaggtc tctttctaca tctcgttgat aatagatcaa agctcgttta
    16141 ctcaaagtat ttttatattc attggcaata taatctagat aattttgtgt atccttatct
    16201 agaaataatt ttttctcaat ggcattggca cgtgcagcta acgtagtttt atctgtgatg
    16261 gttgctactt gatccatagg attattgtct aagtatggtt ttcctagagg attattggaa
    16321 cttttcgaaa gatatttaaa tagaaccttt aatgccgtga tatttctatt gatagtagaa
    16381 gtagatagac ctaagttatt attatctttg agcttagttt ttgttgtctc tttttttaca
    16441 ttttcttttg tgcgacgttt caaatgagat ttgtacaagt ttaggtcata ggcagttaac
    16501 tgtcttatcg aggtgataga agtatcagga acgatagact cactcagaag ccattcataa
    16561 aagagcttat aat
//
LOCUS       DACDKM010000059        16491 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20834_2016
            SAMN12640370-rid9891663.denovo.059, whole genome shotgun sequence.
ACCESSION   DACDKM010000059 DACDKM010000000
VERSION     DACDKM010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640370
            Sequence Read Archive: SRR10040891
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16491)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16491)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742835.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742835.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:04:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,012
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 29 of 75
            Pseudo Genes (incomplete)         :: 33 of 75
            Pseudo Genes (internal stop)      :: 19 of 75
            Pseudo Genes (multiple problems)  :: 5 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16491
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640370-rid9891663.denovo.059"
                     /isolate="E20834_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(27..725)
                     /locus_tag="IV334_001724"
     CDS             complement(27..725)
                     /locus_tag="IV334_001724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobyric acid synthase"
                     /protein_id="HAP8943792.1"
                     /translation="MANYELNIAHLYGNLMNTYGDNGNLLMLKYIAEKMGVSCHTEIV
                     SIHEPFDADKYDLVFFGGGQDFEQLIISKDIQKKKDSLINYIENDGVMLAICGGYQLL
                     GHYYMGANGEKIQGISALDHYTLSQENNRFIGDIVIHNEEFNETYYGFENHNGRTFLG
                     EGERPLGKVVQGQGNNGEDQSEGVIYRNVFGSYFHGPILARNENLAARLIRLALEQRY
                     GKELDLPYLEGQTA"
     gene            complement(718..2070)
                     /locus_tag="IV334_001725"
     CDS             complement(718..2070)
                     /locus_tag="IV334_001725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HAP8943793.1"
                     /translation="MGIRSHFAIAVGKTSQWALKTFFKGGSSLPGKLALKVDPHILDS
                     LAKDYQVVVVTGTNGKTLTTALTVNILRQQFDEVLTNPTGANMVQGIVSTFLQAKPKK
                     GQKKFAVLEIDEASLSKVTEYMKPELFLFTNIFRDQMDRYGEIYTTYKMIVDGAAKSP
                     NATIISNGDSPIFNSIETVNPRKYYGFDHEEDREQMAHYNTDGVLCPNCHHILHYKMI
                     TYANLGKYYCPNCDFKRPELDYRLTDMVRMDNVSADFVIDGHEYGIEVGGMYNVYNAL
                     AATAVAEYFNVDPDKIKQGLGYDEKVFGRQEVININGKKCTLVLVKNPVGINQVIDMI
                     GLAPYPFSLVALLNANYADGIDVSWIWDGNFENFADMDIPAVISGGDRHTDMTLRLKV
                     AGIPENKLTETKELPEVIEKIKELPTEHVYILATYTAVLQLRKELAAQGFIKGGMNRG
                     "
     gene            complement(2293..3693)
                     /locus_tag="IV334_001726"
     CDS             complement(2293..3693)
                     /locus_tag="IV334_001726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HAP8943794.1"
                     /translation="MHVDLFFRRLQRRGQKFAANHFSSIQIIVFYYILMTVLSLVLFY
                     MPIFREPDSHVSFVDMLFMAISTVSVTGLSTFDINSVFNDNGIILLEILFQVGGLGIM
                     MISTAFVIFSKRRITLKQRQLIMTDMNQPRLSGIVRLIRITFAILIWFQLLFGTFFSI
                     YFYYRGYFDRWRDAVFYGFYQAISAVTNSGFDVTGDSIKPFAHDYFFLILIMFLIFVG
                     GIGFPVLMECREWLLYKRSNAKLPFRFSLFTKLAVLAFVILFVSGTVLIYLLEKDHLF
                     QDSNMSVKWINSMFYSITTRNAGLQIHDLGDFQITTLIIFSLLMFIGCSPSSVGGGIR
                     TTTVAIIGLYLYSFLKSEDNINIFGRRIDQDDVRKSVVVFMLSLGMCFFCIVFLSATE
                     EQTLISIIIEVTSAFGTTGLSLGITGDLSVVGKITIATLMFIGRIGMLYTLMLFVPKE
                     TRDLGYEYPSEKIIIG"
     gene            3854..4591
                     /locus_tag="IV334_001727"
     CDS             3854..4591
                     /locus_tag="IV334_001727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase QueH"
                     /protein_id="HAP8943795.1"
                     /translation="MINAQQIVEKMKNQKINYDTVLKKMIMQWEKEEVKPKILIHTCC
                     APCSTHTLEFMSQYAEITLFFSNSNIHPKSEYLRRLHEQKRFVDQFNEKTDHHVALIV
                     DEYRPNEFTKMVLTNHLENEKEGGARCSACFNMRLDLVAQKAQELGYDYFGSALTISP
                     KKNAALINQIGMDIQKIYGTQYLPSDFKKNKGYERSLQMCKEYDIYRQCYCGCVFAAK
                     QQGCDLKEINREAKAYLKTSTVVFEEN"
     gene            4716..5195
                     /locus_tag="IV334_001728"
     CDS             4716..5195
                     /locus_tag="IV334_001728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A-agglutinin attachment subunit precursor"
                     /protein_id="HAP8943796.1"
                     /translation="MRKVAPILKPIAVLTISTIVATTTGIVNSSSYRAPSSSSSTVEE
                     TTPTTSVSTSEPTTTESLNETTTTSTWDDTTTSSSYDETYTTDSSSETSNTVPDGTPS
                     DSTNPSTSPDTSASSETIPSSTTVDDNTQNDDAARTAQQNQDSGNTQTYNQQAGDSQ"
     gene            5192..5959
                     /locus_tag="IV334_001729"
     CDS             5192..5959
                     /locus_tag="IV334_001729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8943797.1"
                     /translation="MTFLLDLINGVHKFLGYLDISPKYLNRGYTILSVIPTLYLLRIV
                     YGLWQNQNYLQFFLYGLAFLVLVYFTVLNVFYYFLDKNTKADVTQLFVKYLPDEAFNI
                     QTEESIDNGNSIDKVNTQEVMVSYDEDYQLKLAENMRYLIGNGEIKTNELGRMDGFLV
                     DRNTLYPYYYVKKTGEKEYVLNIGRSYSDMEKVGTIRLDSTEDTLEPVGLFIVGGDFV
                     KDGLRYHEPYRLKLIAKKAQSQEEPVVYSRSQRRRHS"
     gene            6189..6758
                     /gene="lepB"
                     /locus_tag="IV334_001730"
     CDS             6189..6758
                     /gene="lepB"
                     /locus_tag="IV334_001730"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HAP8943798.1"
                     /translation="MKEFLKNWGILILVLAAILLARVYVFTPVTVNGHSMDPTLSDGQ
                     RLISSKISNYERMDIITTKEPGDEERMIVKRIIGMPGDTVKMENDQLTINGKKYDEPY
                     LDEFKKEFSEDKLQGEYAYSSGFQAQAESSSTFTSDFEYTVPKGKYLVLGDNRLISKD
                     SRMFGLVDKDMIQGKVVFRYWPLSEIKIM"
     gene            complement(6862..7464)
                     /locus_tag="IV334_001731"
     CDS             complement(6862..7464)
                     /locus_tag="IV334_001731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HAP8943799.1"
                     /translation="MTKNIFDSIANHYDSPDRQALAKIIREELTTYLPRDSHQKVLLD
                     YGGGTGLVSLPLAERFKELIIADASETMLKMAEEKIQAADLKNVRTIHADASVEFPAV
                     QANLILLSLVLLHIPDTENILTKLYEILAPGGQLIIVDFDKNEQISHPKVHNGFSQEE
                     LNNRLEKTGFVSATSHTFHRGEKLFMNKNASLFLSISQKA"
     gene            7570..8039
                     /locus_tag="IV334_001732"
                     /pseudo
     CDS             7570..8039
                     /locus_tag="IV334_001732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325408.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
     gene            8145..8729
                     /locus_tag="IV334_001733"
     CDS             8145..8729
                     /locus_tag="IV334_001733"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HAP8943800.1"
                     /translation="MAQLFFKYGAMNSGKTIEILKVAHNYEEQNKPVVLMTSGIDTRD
                     GLGVVSSRIGLKRNAMPIFEETDVFDVIQKMESKPYCVLIDEAQFLKKKHVIAFTRIV
                     DELDIPVMAFGLKNDFRNELFEGSKYLLLYADKIEEMKTICWFCHKKAIMNLHYIDGN
                     PVYEGDQVQIGGNEAYYPVCRKHYFHPSMITEGE"
     gene            8732..9805
                     /gene="prfA"
                     /locus_tag="IV334_001734"
     CDS             8732..9805
                     /gene="prfA"
                     /locus_tag="IV334_001734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003128515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HAP8943801.1"
                     /translation="MYDQLQAIEDRYEELGELLSDPEVISDTKRFMQLSKEEANTRET
                     VEVYRRYKEVVQGITDTEELLGEKLDDEMAELAKEELSELKKEKEVLEEKIKILLLPK
                     DPNDDKNIIMEIRGAAGGDEAALFAGDLFNMYQKYAEAQGWKTEVMEANVTGIGGYKE
                     VIMMITGDNVYSKLKYESGAHRVQRVPSTESQGRIHTSTATVVVMPEAEEVEIDIEDK
                     DIRTDIYHASGAGGQHVNKTASAVRLTHLPTGIVVAMQDERSQIKNREKAMKILRARV
                     YDKIQQEAQSEYDANRKSAVGTGDRSERIRTYNFPQNRVTDHRIGLTIQKLDQILAGK
                     LDEIVDALIMYDQTSKLEAMQNG"
     gene            9798..10637
                     /gene="prmC"
                     /locus_tag="IV334_001735"
     CDS             9798..10637
                     /gene="prmC"
                     /locus_tag="IV334_001735"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HAP8943802.1"
                     /translation="MGKMTYREVLSRASSFLEDHGKEAYSIQFLFLERKQWKKLDWLL
                     HMNEEISEEEQRLIETDLRLLLADHPPQYLLGYADFYDHRLKVTEATLIPRPETEELV
                     EWCLDETPGVPLEVIDIGTGTGAIAISLKAARKNWHISAVDLSEEALEVAKENAQKEG
                     TKISFYHGDTLGPVMDQQFDVIISNPPYISRNEWELMDESVRSFEPKMALFAENDGLA
                     IYEKIAVEAPSVLKSNGKIFLEIGFRQGEAVKKIFQQAFPDKKVAIKKDLFGNERMIQ
                     VYG"
     gene            10630..10809
                     /locus_tag="IV334_001736"
     CDS             10630..10809
                     /locus_tag="IV334_001736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8943803.1"
                     /translation="MVEVVDEKFFICFFIIKKHLFDKDVNDFLVYQQVYPLLTNRLST
                     NSGKLEDKLRQNKNV"
     gene            10831..11856
                     /locus_tag="IV334_001737"
     CDS             10831..11856
                     /locus_tag="IV334_001737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonylcarbamoyl-AMP synthase"
                     /protein_id="HAP8943804.1"
                     /translation="METKRYDETELQEAAEALKEGELVAFPTETVYGLGANALLPNAV
                     KKVFSVKGRPQDNPLIVHVASFEQVKEYVDNFHPAAEKIVKNFWPGPLTLIFKIKKDT
                     LPSVVTGGLSTAAFRIPDNKKTLEVIELSGVPLVGPSANTSGKPSPTTADHVYHDLQG
                     KITGIIDDGATRIGVESTVLDLSDPTAMPMILRPGAVTKEQIEAVIESPVAIDQHLVK
                     ENETPKAPGMKYKHYSPDTRVLMVRDGDWSTAVQWAKNKKIRAGVIASPEIADQVRTD
                     TAAVYMYNDNSVEAAAKGLFAGLRGLDEPTLGLDLIFVQVYPETGLGNAYMNRLKKAA
                     GQNYFEK"
     gene            12031..13275
                     /locus_tag="IV334_001738"
     CDS             12031..13275
                     /locus_tag="IV334_001738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HAP8943805.1"
                     /translation="MVDYKTFDPDLWAAIAKEEERQEHNLELIASENFVSEAVMAAQG
                     SILTNKYAEGYPGHRYYGGCEFVDIVENLAIDRAKELFGAKFANVQPHSGSQANTAAY
                     LALVEPGDTILGMDLSAGGHLTHGSPVNFSGKTYHFVAYGVDPTTEVIDYNVVRILAR
                     KHQPKLIVAGASAYGRTIDFAKFREIADEVGAKLMVDMAHIAGLVAAGLHPNPVPYAD
                     ITTTTTHKTLRGPRGGMILTNDEALAKKINSAVFPGIQGGPLEHVIAGKAVAFKEALD
                     PAFKEYSEQIIANAKAMVKVFNQAIGTRVISGATDNHLMLIDVRELGINGKEAESILD
                     SVNITVNKNSIPFETLSPFKTSGIRIGTPAITTRGFKEEDAVKVAELVVKALQAKDDN
                     AQLDEVKTGVRELTEKFPLHKK"
     gene            13428..14375
                     /locus_tag="IV334_001739"
     CDS             13428..14375
                     /locus_tag="IV334_001739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAP8943806.1"
                     /translation="MTDRISLEKEAVAFSDANAPHPRIYELAPEEGRVLLEEVQESPV
                     NKYSVDIEDLMVDTGEWGKLNIRLVRPEGNMDKLPVIFYIHGAGWVFGSAQTHDKLIR
                     ELAVRTNSVVVFPEYSRSPEAKYPTAIEQNYAVLQQLNKLAKSKNLDLENLIVAGDSV
                     GGNMATVMTILTKERKGLPISKQLLYYPVTNADFDTDSYHEFGENYFLTREGMMWFWD
                     QYTTNEEERAEITASPLRANINELNDLPSAMILTGEADVLRDEGEAYARKLRDAGVPV
                     TQVRFQGIIHDFVMVNAMDQTKATRAAMALSTSWIKKKE"
     gene            14509..15138
                     /gene="upp"
                     /locus_tag="IV334_001740"
     CDS             14509..15138
                     /gene="upp"
                     /locus_tag="IV334_001740"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013774406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HAP8943807.1"
                     /translation="MGKFQVIDHPLIQHKLTIIRDKNCGTKVFREVVDEIAMLMAYEV
                     SRDMPLEDIVIETPITETTQKTLSGKKVAIIPILRAGIGMVDGILELIPAAKVGHIGL
                     YRDEETLEPHEYFVKLPEDIDARQLFVVDPMLATGGSAIMAIDALKERGASNIKFVCL
                     VAAPEGVKALQEAHPDIDIYTASLDEKLNEHGYIVPGLGDAGDRLFGTK"
     gene            complement(15277..15519)
                     /locus_tag="IV334_001741"
     CDS             complement(15277..15519)
                     /locus_tag="IV334_001741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8943808.1"
                     /translation="MKMATFGKFDTTIVPSEIGEKFKINTHVMFTIHGKSQTGTISKQ
                     LKNSAVVEIDETAENANLMTQSKGVVIVNYKELKKI"
     gene            15640..15712
                     /locus_tag="IV334_001742"
     tRNA            15640..15712
                     /locus_tag="IV334_001742"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:15673..15675,aa:Thr,seq:cgt)
     gene            complement(15831..16469)
                     /locus_tag="IV334_001743"
     CDS             complement(15831..16469)
                     /locus_tag="IV334_001743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HAP8943809.1"
                     /translation="MNNILGKKIELKKGILGTIVVVLGLLGLLFAPSIISIFSLTITG
                     FIAFTLVFGKKDTKKMFSKPVAPVKNIAKYFFINVVISAAVSVFLQQILKWGLTGNPI
                     NEAFSPLLFIILPIMILGEELFSVYFLAIFSSKFSIPVASFLSAIIFGFIHYSTYDNG
                     NILHTVAHILLIQGVARLLFNQAAIKSNSIITSWAVHVIFDFTAILLVVLFS"
BASE COUNT         5622 a         2947 c         3316 g         4606 t
ORIGIN      
        1 ttgtcacatt cccctttttc gaataactag gctgtttgtc cttcaagata gggcagatcc
       61 aattctttac cgtaacgttg ttctaaagct aggcggatca aacgagctgc caggttctca
      121 tttcttgcaa ggataggtcc atggaaataa gaaccgaaga catttcggta aatgacacct
      181 tcagattggt cttcaccatt atttccttga ccttgtacca ctttccccaa aggtcgttca
      241 ccttcaccta agaaagtccg accattgtgg ttttcaaatc cataataagt ttcattgaat
      301 tcttcgttat ggataacgat atcaccgata aaccggttat tttcctgact taacgtgtag
      361 tgatccagtg cactgattcc ttggatcttt tctccattag ctcccatgta gtagtgacct
      421 agcaattggt agccaccaca aatggctaac atgacgccat cattttcgat atagttgatc
      481 aaagaatctt tctttttctg gatgtctttt gaaatgatta attgctcaaa gtcttgacca
      541 ccgccaaaga aaaccaagtc atatttatca gcatcaaaag gttcatgtat actaacgatc
      601 tccgtatggc aagaaacccc cattttttct gctatatatt tcaacatcaa aaggtttccg
      661 ttatcaccat atgtgttcat caaatttcca taaaggtggg cgatattgag ttcataatta
      721 gccacggttc attcctcctt tgataaatcc ttgagcagct agttctttac gaagctgcaa
      781 tacagcagtg taagtagcta aaatatagac atgctcagtt ggcaattctt tgattttttc
      841 gattacttct ggtaattctt ttgtttctgt caatttattt tctggaatcc cggcaacttt
      901 tagacgtaaa gtcatatctg tatgacggtc tcctccagaa ataacagctg ggatatccat
      961 atcggcaaag ttttcaaaat taccatccca aatccagctg acatcgattc cgtctgcgta
     1021 attggcattt aataaagcca ctaaagaaaa tggataagga gctagaccaa tcatatcgat
     1081 tacttgattg atacctacag gatttttgac aagtactaat gtacattttt tcccattgat
     1141 attgatgact tcttgtcggc cgaatacttt ctcgtcatat cctaaacctt gtttgatttt
     1201 atcaggatcg acattgaagt actctgccac tgcagtagct gctaaagcat tgtagacatt
     1261 atacataccg ccaacttcga ttccgtattc atgaccatcg atcacgaaat ctgctgacac
     1321 attgtccatc cgtaccatat cagttaatcg ataatccaat tcaggacgtt tgaagtcaca
     1381 atttggacag taatatttcc ctaagtttgc ataagtgatc attttataat gcaagatgtg
     1441 atgacaattt ggacataaga caccgtctgt attgtaatga gccatttgtt cacgatcttc
     1501 ttcatggtca aagccataat attttctagg attgactgtt tcgatagaat tgaagattgg
     1561 tgaatcaccg ttactgataa tcgttgcatt tggtgacttt gcggcaccgt ctacaatcat
     1621 tttataggtc gtatagattt ctccataccg atccatttga tcacggaaaa tatttgtaaa
     1681 taagaacagt tctggtttca tatattctgt gactttgctc aaacttgctt catctatttc
     1741 taggacagca aactttttct gtcctttttt tggttttgcc tgcaagaaag tcgatacaat
     1801 tccctgtacc atattcgcac cagtagggtt ggttagaact tcatcgaatt gctggcgcaa
     1861 aatattgact gttaaagcgg tagtcaatgt tttaccgttt gttccggtga caacgacgac
     1921 ttggtagtct tttgccaaac tatctagtat atgtggatcg actttcagcg ctagtttacc
     1981 tggtaaactt gatccgcctt taaaaaaagt ttttaatgcc cattgcgacg tttttccaac
     2041 tgcaatcgca aaatgactgc gtattcccat tatgtaacct ccaaaaacga tttgctgacg
     2101 aataaattca actaatcata ccatatgttg aaaaaaaggt taaattgctt ttcttattct
     2161 taagaaaagg ttacggctga aagtgcatga aatcggacaa aatcgcatga tttaacaaaa
     2221 aaacagcttg aaacaaaatg attgtttcaa gctgctgtga ttttattatt ccctcttacc
     2281 tatattccca ttttaaccga taataatttt ctcgcttgga tattcatagc ctagatctcg
     2341 tgtttctttt ggcacgaata acatcaatgt atatagcatt ccgatccgcc caataaacat
     2401 cagtgtagca atcgtgattt tgcccacaac agacaagtct ccggtaatcc ctaaagataa
     2461 accagttgtt ccaaacgcag aagttacttc gatgatgatc gagatcagcg tttgttcttc
     2521 tgtagcagat aaaaaaacga tacaaaagaa acacatacca agagaaagca taaagacaac
     2581 gactgatttt cgcacatcat cttgatcgat tctccgtcca aaaatattga tgttatcttc
     2641 acttttgagg aaggaatata gatacaaacc gatgattgct acagtagttg ttcgaatccc
     2701 gccgcctaca gaactaggac tgcatccgat aaacatcagt aaagaaaaaa tgatcagtgt
     2761 tgtgatttga aaatcaccca aatcgtgaat ctgcagacca gcattacgag tcgtaatcga
     2821 ataaaacatc gaattgatcc atttgacact catattggaa tcttggaata aatggtcctt
     2881 ttccaataga taaatcaaga cagttcccga aacaaacaag ataacaaaag ctaacacagc
     2941 taatttggta aacaaggaaa aacgaaacgg aagcttcgca ttggaacgtt tgtataatag
     3001 ccattcccgg cactccatca atacaggaaa accaattcct ccgacaaaaa tcaaaaacat
     3061 gatcaggatc aagaaaaaat agtcatgtgc aaagggcttg atggaatccc cagtgacgtc
     3121 aaacccagag ttcgtgacag ccgatatcgc ctgataaaaa ccgtaaaaaa cggcatcacg
     3181 ccaacgatca aaatagcctc tgtaataaaa ataaatagaa aagaatgtcc caaaaagtaa
     3241 ttgaaaccag attaaaatag caaaagtgat ccgaatcagt cggacgatac cagacaaacg
     3301 cggttgattc atatccgtca tgatcagctg tcgctgtttc agtgtgatcc gtcgtttaga
     3361 aaaaatgaca aaagcagtag aaatcatcat gatccccaaa ccaccaactt gaaataatat
     3421 ttccagcagt ataataccgt tatcattgaa cacagaattg atatcaaaag tcgatagtcc
     3481 agtcacactc actgtactga ttgccatgaa cagcatgtcc acaaaagaaa catggctatc
     3541 tggttcgcga aaaatcggca tataaaatag aacgagtgat aagaccgtca tcaatatata
     3601 ataaaaaacg atgatttgga tcgatgagaa atgattggca gcaaacttct gtcctcgccg
     3661 ctgcagcctt cgaaagaata aatcaacatg cacagtacta tcctcctgac aatagtcgtt
     3721 tctaccagta tacaagggga attaggaata ctcaaagaaa ttatcaaaca gataacaata
     3781 cagctttcga ttaaaataga aatggcttat aataaggaaa atcctagaaa aaagaaggag
     3841 gaggaataac aaattgatta atgctcaaca aatcgtagaa aaaatgaaaa atcaaaaaat
     3901 aaattatgat acagttctga aaaaaatgat catgcaatgg gagaaagaag aagtaaagcc
     3961 taagatatta atacatacat gctgtgctcc ttgcagtacg cacacattgg aattcatgtc
     4021 tcaatatgca gagatcacgt tatttttttc aaattcgaat attcatccta aaagtgagta
     4081 tttgcgaaga ttacatgaac aaaaacgatt tgtagaccaa tttaatgaga aaactgacca
     4141 tcatgttgcg ctaatcgttg atgaataccg tccaaatgaa ttcaccaaaa tggttttaac
     4201 aaaccatctt gaaaacgaaa aagaaggtgg tgctcgctgc agtgcatgct tcaatatgcg
     4261 attagatctt gttgcgcaaa aagcgcaaga actaggatac gattactttg gaagtgccct
     4321 gaccatctcg ccaaagaaaa atgcagcttt gatcaatcag atcggaatgg atatccaaaa
     4381 gatttacggg acacaatact tgccaagtga tttcaagaaa aacaaaggct atgaacgctc
     4441 tttgcaaatg tgcaaagagt atgatattta tcgccaatgt tattgtggtt gcgtgttcgc
     4501 tgccaagcag caaggatgcg atctgaaaga aatcaatcgg gaagcaaaag cttacttaaa
     4561 aacatcaacc gtagtttttg aggaaaatta aagaaatctt tcggaaaaac gtaagaaaaa
     4621 aataaagaat ttatggtaga aataaggtag actgtataat ttgtccgtag taaagtaagg
     4681 aaacaagaat aaatttcaat gaaggaagag ataagatgcg aaaagtagca cctattttga
     4741 agccaatcgc tgtattaaca atctcgacga ttgtcgcgac tactacaggt attgtgaata
     4801 gttccagcta ccgtgcacca agttcaagtt ctagtactgt agaagaaaca acaccaacta
     4861 cttcagtctc gacatctgaa ccaacaacaa cggaaagttt gaatgaaacc actacgacaa
     4921 gtacatggga cgatacaaca actagttcgt cgtatgatga gacatataca actgacagct
     4981 caagcgaaac aagtaatacg gtaccagatg gcactccatc ggattcaacc aatccaagta
     5041 caagtccgga tacaagtgct agctccgaaa cgataccaag cagtacgact gtagatgata
     5101 acacgcaaaa tgatgatgca gcacgtacag cacagcaaaa ccaagattca ggaaataccc
     5161 aaacgtataa ccagcaagca ggtgattctc aatgacattt ttactagacc ttatcaacgg
     5221 ggtgcataaa ttccttggct atttagacat cagtcctaaa tatcttaacc gtggatatac
     5281 gattttaagt gtgattccca cgctttattt attgagaatt gtttatggac tttggcaaaa
     5341 ccaaaactat cttcaatttt tcttatatgg acttgcattt ctagtgcttg tttattttac
     5401 tgttttaaat gtattttatt actttttaga taaaaacacg aaagccgatg tcactcagct
     5461 ttttgtgaag tatttacctg atgaagcgtt caacatccag acagaagaat cgatagataa
     5521 tggaaattcg atcgataaag tgaatacaca agaggtcatg gtttcttatg acgaagatta
     5581 tcaattgaaa ttagccgaga atatgcggta tttgattgga aatggtgaga tcaaaacaaa
     5641 tgaactcggt cgcatggatg gatttctggt cgatagaaat acattgtatc cttattacta
     5701 tgtcaagaaa acaggagaga aagagtatgt tctaaatatt ggacgaagct actcggacat
     5761 ggaaaaagta gggaccattc gacttgactc aacagaagac acattggaac ctgtcggatt
     5821 gttcatcgtc ggcggtgatt ttgtcaaaga cgggttacgc tatcatgagc cttatcgtct
     5881 gaaattgatt gcaaaaaagg cacagtcgca ggaagaacca gtggtttata gtcgtagcca
     5941 aagaagaaga cattcataat gtgatgaaac gccgtgtatc agtggttgcg ataaaaacgt
     6001 ttattcggaa taagggggat gtgacacgtc ttttgtcacg ttcctttttt atataacgag
     6061 aaagataaga cgaacacatc acttattctt tcctttcatt tataaacatt gtagaataaa
     6121 gaaagtacaa cgaagtattg tgaaaaaacg taataaataa cgaggaaaat acaagtagga
     6181 ggtaagctgt gaaagaattt ttgaaaaatt gggggatttt gatccttgta ttagcggcta
     6241 ttttattagc tagagtgtac gtgtttacac cagttactgt aaacgggcat tcgatggacc
     6301 ctacattaag tgatggacaa cgattgattt catctaagat ctcaaactat gaacgaatgg
     6361 atattattac tacaaaggaa ccgggggatg aagaacggat gattgttaaa cggatcatcg
     6421 gtatgcctgg agatacagtc aagatggaaa atgaccagtt gaccattaat ggcaaaaagt
     6481 atgatgaacc ttatctggat gagttcaaaa aagaattttc agaagataag ttacaaggag
     6541 aatatgctta tagctcagga ttccaagcac aagcagaaag ttcatccacc tttacgagtg
     6601 actttgaata tactgtgcca aaaggaaagt atcttgttct aggcgataac cgcttgattt
     6661 caaaagacag ccgaatgttt gggttagtcg acaaagatat gatccaagga aaagttgttt
     6721 tccgttattg gcctctttca gaaattaaaa tcatgtgatg aaaaaataaa aagaagctgt
     6781 gacatgtttt ttgtcacagc ttctttttcc gaaaattcct tcttatttct tggagttgct
     6841 cacttttttt gcaatctcat tttaagcttt ttggctgatg gataagaaaa gcgaagcgtt
     6901 tttgttcata aatagctttt ccccacgatg gaaggtatga ctagtagcag agacaaagcc
     6961 cgttttttcc aatcgattat tcaattcttc ttggctaaag ccattatgga ctttcggatg
     7021 actaatttgt tcattcttgt caaagtctac gatgatcaat tgaccgccag gagctaaaat
     7081 ttcataaagt tttgtaagaa tattctccgt atcaggaatg tgtaaaagta caagtgatag
     7141 aaggatgaga ttagcttgga cagcaggaaa ttcgacagaa gcatcagcgt ggatcgttcg
     7201 gacatttttc agatctgctg cttggatctt ttcttctgcc attttcagca tagtttctga
     7261 agcatcggca atgatcagtt ctttgaatct ttccgcaagc ggtaaactaa caagcccagt
     7321 tccgcctccg taatccagca ggactttttg gtggctgtcg cgcggtagat aagtagttag
     7381 ttcttcgcga ataatcttgg ctaatgcttg acggtcaggg gaatcataat ggttagcgat
     7441 ggaatcaaaa atattttttg tcatcaaata gctccttacg taaaactttg aaattttaac
     7501 ttattatagc acgtgaaaag aaagaaagca tgctatactc aattcaaaaa gggagaatct
     7561 agaaagaata tgccgatatc gtttgtaaaa gatagagagg aaaaaggaaa gtgtgtccga
     7621 gaaatattac tagacttgcc tgagtggttt gggcttcctg aatcaacgga aaaatatatc
     7681 gaggaatcaa gtaaactacc tttatggtgt gaaaaaagaa aagaagaata tttaggcttt
     7741 atcactcttt cacagacaag tgaagatact gccgaaatct attctattgt atgggaataa
     7801 aaaaagacta tcatcaccaa ggattaggaa caaaactttt taaagaagca gaaggttacg
     7861 cagcaaaaca ttacaaatat ttgcaagtga agactgtaga cgaggggcac tactctatat
     7921 atgatcaaac tatttgtttc tacgaatcat taggattttc aaggttggaa gtttttccaa
     7981 atttatggga tgaatggaac ccatgtttag ttcttgtcaa aaaattggaa caaaagtgag
     8041 caatagattt aatactaatc tgtgcttgat gaaaattctc actgtaaaaa atgctgaaaa
     8101 tcaagtataa taccatagga acaaactgga aggtgaaaaa gagcatggca caactatttt
     8161 ttaagtatgg cgcaatgaat agcggcaaga cgatcgagat cctaaaagtc gcacataatt
     8221 atgaagaaca aaataagcca gttgtcttaa tgacaagcgg tattgataca agagatggtt
     8281 taggcgttgt ttcaagccga atcggactga aacgtaatgc gatgccgata tttgaagaaa
     8341 cagacgtatt tgacgtaatt cagaaaatgg aaagcaaacc ctattgtgtg ctgatcgatg
     8401 aagcgcaatt cttaaaaaag aaacatgtca ttgcttttac gagaattgtg gacgaattgg
     8461 atattccggt tatggcattt ggtttgaaaa atgattttcg aaacgagtta tttgaaggat
     8521 caaaatattt attgctttat gcagacaaaa tcgaagaaat gaaaacaatc tgttggttct
     8581 gccacaaaaa agcaatcatg aatttacatt atattgatgg aaaccctgtt tatgaaggcg
     8641 atcaagtgca aattggcgga aatgaagctt attaccccgt ttgtcgaaaa cattactttc
     8701 acccatcaat gattacagaa ggagaataag catgtacgat caattacaag caattgaaga
     8761 ccgttatgaa gagcttggag aacttttaag tgacccagaa gtgatttctg atacaaagcg
     8821 ttttatgcag ctttcaaaag aagaagcaaa cacgcgcgaa acagtcgaag tttatcgtcg
     8881 ttataaagaa gtagtacaag gaatcaccga tacagaagaa ctgcttggtg aaaaactgga
     8941 tgatgaaatg gctgaactag caaaagaaga attatctgaa ttaaaaaaag aaaaagaagt
     9001 cttagaagaa aaaatcaaga tcttgctttt accaaaagat ccaaatgacg ataaaaatat
     9061 catcatggaa atccgtggag cagcaggcgg agacgaagca gcattattcg ctggcgatct
     9121 tttcaatatg taccaaaagt atgctgaagc acaaggctgg aaaacagaag tgatggaagc
     9181 taacgttacc ggaatcggtg gatataaaga agtgatcatg atgatcaccg gagacaacgt
     9241 atattcgaaa ttaaaatatg aaagtggtgc gcaccgcgtc cagcgtgttc cttcaactga
     9301 atcgcaaggg cgtatccata cttccaccgc aacagttgtc gtgatgccag aagctgaaga
     9361 agtagagatc gatattgaag acaaagatat ccgtacagat atctatcatg cttcaggtgc
     9421 tggcggacag cacgtcaata aaacagcatc tgctgtacgt ttgactcact taccaacagg
     9481 gatcgttgtt gctatgcaag atgaacgttc tcaaatcaag aaccgtgaaa aagcaatgaa
     9541 aatcttgcgt gctcgtgtgt acgataagat ccagcaagaa gcacaaagcg agtatgatgc
     9601 caatcgaaaa tcagcagtcg gaactgggga tcgttcagaa cggatccgga cgtataattt
     9661 cccgcaaaat cgtgtgactg atcatcgtat cggattaact atccaaaaat tagatcagat
     9721 tttagcaggg aaattagatg agatcgttga tgcattgatc atgtatgatc aaacatcaaa
     9781 actggaagct atgcaaaatg ggtaaaatga cctatcgaga agtcctgtca cgggcttctt
     9841 cttttttaga ggatcatggg aaagaagcat atagtatcca atttttattt ttggaacgaa
     9901 aacaatggaa aaaacttgat tggctccttc atatgaacga agagatttca gaagaagaac
     9961 agcggttgat cgaaaccgat ctgcgacttc tgcttgcaga tcatccacct cagtatttgc
    10021 taggatatgc agatttttat gatcatcggt taaaggtaac tgaagcaaca ctgatccctc
    10081 gaccagaaac agaggagtta gtcgaatggt gtttagacga gacgcctggt gtaccgttag
    10141 aagtgataga tattgggaca gggactggag caattgctat tagtttgaaa gcagcaagaa
    10201 aaaattggca tatatctgct gtggatcttt cagaagaagc attagaagta gcgaaagaaa
    10261 atgctcagaa agaaggaacg aaaatcagtt tttatcatgg agatacatta ggaccagtaa
    10321 tggatcagca atttgatgtg attatttcta atccgccgta catcagtcgg aatgaatggg
    10381 aactgatgga tgaaagtgtc cggtcattcg aacctaaaat ggcccttttt gctgagaacg
    10441 atggattagc tatttatgaa aaaatagcag ttgaagcacc aagcgtacta aaatcaaatg
    10501 gaaagatttt tttagaaatc ggatttcgcc aaggtgaagc agtaaaaaaa atatttcagc
    10561 aagcatttcc agataaaaaa gtggcgataa aaaaggatct ttttggcaat gaacgaatga
    10621 tccaagttta tggttgaagt tgtggatgaa aaatttttta tttgtttttt tattataaaa
    10681 aaacacttat ttgacaagga tgtaaacgac tttttggttt atcaacaagt ttatccacta
    10741 ttgacaaata gattatccac aaacagtgga aaacttgagg ataaactcag acaaaataag
    10801 aatgtatgaa aaaggggttg tggataaact gtggaaacga aacgatatga tgaaacagaa
    10861 ttgcaagagg cagccgaagc actgaaagaa ggagaacttg ttgcttttcc taccgaaaca
    10921 gtatacggat tgggcgccaa cgctctttta ccaaacgctg tcaagaaagt gttttctgta
    10981 aaagggagac cgcaagataa tccattaatc gtccatgtcg cctcttttga acaagtaaaa
    11041 gagtatgtgg acaactttca tccggcagca gagaagattg tgaagaactt ctggcctgga
    11101 ccattaactt tgatatttaa gatcaaaaaa gacactttgc cctcagttgt gacgggagga
    11161 ttatccacag ctgcatttcg tataccggat aacaaaaaaa cactagaagt aatcgaactt
    11221 tccggtgttc cgcttgtcgg tccaagtgca aatacgtcgg gaaaaccaag tccaacgact
    11281 gcagatcatg tatatcatga tctacaagga aaaattaccg ggattattga tgatggagct
    11341 acgcgaattg gtgtagaatc cactgttctt gatttaagtg atccaacagc catgcctatg
    11401 attttacgac caggtgcagt aacgaaagaa caaatcgaag cagtcatcga atctcctgtt
    11461 gctattgatc agcatcttgt aaaagaaaat gaaacaccaa aagcccctgg gatgaaatac
    11521 aaacactact cacccgatac acgtgttctg atggtcagag atggcgattg gtcaactgca
    11581 gttcaatggg caaaaaataa gaagatccgt gcaggtgtca tagcaagccc cgaaattgcg
    11641 gatcaagtgc ggacagatac tgcagctgtt tatatgtata acgacaactc agtagaagcg
    11701 gcggctaaag gattgtttgc aggtctaaga ggattagatg aaccaacact tggattagat
    11761 ttgatttttg tacaagtgta tccagaaact ggattgggga atgcttacat gaatcgcttg
    11821 aaaaaagcag caggacaaaa ttattttgaa aaatgatcaa ttggttattg tcgcattctt
    11881 ttagtatgat acaatgctat caagtaaatc ttctatcagt gaacgaaaaa ctaagcgtaa
    11941 ctaatcgtaa tagaagagat acgaatattt ggcaatttgt ttttgatggg aaaaataaaa
    12001 ttgtaggaaa ataaaaagga gctgttgttt gtggtagatt acaaaacgtt tgaccctgat
    12061 ctttgggctg cgatcgccaa agaagaagaa agacaagaac ataatttgga gctgattgcc
    12121 tctgaaaact ttgtctccga agcagtgatg gctgcacaag gaagtatctt gacaaataaa
    12181 tacgcagaag gttatccagg gcatcgctat tatggtgggt gtgaatttgt cgatatcgtt
    12241 gaaaatttag cgattgaccg tgcaaaagaa ttattcggag cgaaatttgc gaacgtccag
    12301 cctcactccg gctctcaagc caacacggct gcttatctcg ctttggtaga accaggggac
    12361 acgatcttag gaatggatct ttccgcaggc ggacatctaa cacatggatc accagtcaac
    12421 ttcagtggca aaacctatca ctttgttgct tacggtgttg atccgacaac agaagttatt
    12481 gattataatg tggtacgtat tttagctaga aaacatcaac caaaattgat cgtagcaggt
    12541 gccagtgctt acggacgaac gatcgacttt gcgaaattca gagaaatcgc agatgaagta
    12601 ggcgcaaaac tgatggtaga tatggcacat atcgcaggat tagtagcagc aggacttcat
    12661 cctaatccag ttccttatgc agatatcaca acgactacca ctcataaaac attgcgtggc
    12721 cctcgaggcg gcatgatttt gacaaacgat gaagcattag caaaaaaaat aaatagtgcg
    12781 gtatttcctg gtattcaagg cggccctcta gagcatgtga ttgcgggtaa agcagtagca
    12841 ttcaaagaag cattagatcc tgcatttaaa gaatatagtg aacaaatcat cgccaatgca
    12901 aaagcaatgg tcaaagtttt caatcaagca attggaacca gagtcatttc aggggcgaca
    12961 gataatcatc tgatgttgat cgatgttcgc gagttaggta taaatggaaa agaagcagag
    13021 agcattttgg acagtgtgaa tatcactgta aataaaaact cgattccttt tgaaacacta
    13081 agtccattca aaacaagcgg tattcgaatc ggcacgccag caatcactac tcgcgggttc
    13141 aaagaagaag atgctgtaaa agtggctgaa ctagttgtca aagcattaca agcaaaagat
    13201 gataacgctc aactagacga agtcaaaaca ggtgtacgcg aattaactga aaaatttccg
    13261 ttgcataaaa aataaaaatt taaattccat tagtaaacag aaaagttcaa tgttgggctt
    13321 ttctgtttat tttttgggaa aacggattct ttgacaatat cggtgatgct ctctagtata
    13381 tgaggtgtaa ttttagtctt cacactaaat ctcagggagg aaaagtcatg actgatcgta
    13441 tctcgttaga aaaggaagca gtagcattca gtgatgcaaa tgcgcctcat ccgcgtatct
    13501 atgaactagc accggaagaa ggaagagttt tactagaaga agtacaagaa tcacctgtga
    13561 acaaatattc cgtcgatatt gaagacttga tggtggatac tggcgaatgg ggaaaactca
    13621 atattcgtct cgtacgacca gaagggaata tggataaatt accagttatt ttttatattc
    13681 atggagcagg ctgggttttt ggcagtgctc aaacccatga taaattgatc agagaacttg
    13741 cggttcgtac gaattccgtc gttgtctttc cagaatatag tcgatcacca gaagcaaaat
    13801 atccaacagc aattgaacaa aattatgctg tgttgcaaca gctgaataaa ttagccaagt
    13861 caaaaaatct ggatctcgag aatctaatcg ttgcaggaga ttcagtaggt ggtaacatgg
    13921 ctacagtcat gacgatctta acaaaagaac gaaagggcct cccaatatcc aagcaattgc
    13981 tgtattatcc agtcacaaac gctgattttg atacagattc ctatcatgaa ttcggagaaa
    14041 actatttttt gacaagagaa ggaatgatgt ggttttggga ccaatatacg acgaatgaag
    14101 aagagcgtgc tgaaatcaca gcttcgcctt tgagagcaaa tataaatgaa ctaaatgatc
    14161 ttccttcagc aatgatttta acgggagaag cggatgtatt aagagatgaa ggtgaggctt
    14221 atgcgcgcaa acttcgtgat gcaggggttc ctgttacaca agtacgcttc caaggaatca
    14281 tccatgattt tgtcatggta aatgctatgg atcagacaaa ggcaacgcgt gcagcaatgg
    14341 cattatctac ttcttggatc aagaaaaaag aataagcttt gcttgtccgc aggaaaactt
    14401 cttttcaaaa acggattttc ttaggaaaaa tggcgagttt gactagtcaa tcacttgatt
    14461 ttgttttaca attaaaaaga aacgacagaa caaaggagag cttaaaaaat gggcaaattt
    14521 caagtgatcg atcatccatt gattcaacac aaactaacta ttattcggga caaaaactgc
    14581 ggaacaaagg tttttcgtga agtagtcgat gaaatcgcta tgttgatggc atatgaagta
    14641 tcacgggata tgccattgga agacatcgtg atcgaaacac cgatcactga aacaacacaa
    14701 aaaacgttgt ctggtaaaaa agtagcgatt attccaatct tgcgcgcagg tattggaatg
    14761 gttgacggta tcttggaatt gattccagct gctaaagtgg gacatatcgg tctttatcga
    14821 gatgaagaaa cattggaacc acatgaatat ttcgtcaaac ttccagaaga cattgatgca
    14881 cgtcagttgt ttgtagtaga tccaatgttg gcaacaggcg gatcagcaat catggcaatc
    14941 gatgcattga aagaacgtgg cgcgtcgaac attaaatttg tttgtttggt agcagcacct
    15001 gagggagtaa aagctttgca agaagcgcat cctgatattg acatctatac agcttctcta
    15061 gatgaaaaat tgaatgaaca tggttatatc gttcctggac taggggatgc gggtgaccgt
    15121 ttgtttggta caaaataagt aaatacgaaa aagggtgtga cacaactttt gtcacatcct
    15181 tttttggtca tttttaacgt ttgatgcaga acagcttttt cacagctttc gtttgcatat
    15241 aaaaaaacca tcccaaaaat gcagagatgg tcaaattcaa atctttttta gttctttgta
    15301 gttaacgatt actacaccct tactttgcgt catcaagttc gcattttctg cagtttcatc
    15361 aatctcaaca acagcggaat ttttcaattg ctttgaaatc gttccagttt gcgacttgcc
    15421 gtgaattgtg aacattacgt gagtattgat cttgaatttt tcaccaattt ccgatggtac
    15481 gatagttgta tcaaattttc caaaagttgc cattttcata aaacctgcct ttcacatcaa
    15541 ttataccacg ttttatgaaa aatatcatga aattaaacta attttccatc ggactcttta
    15601 caatcagcca aatagagtat ataatttctt ttggataagg ccagcttagc tcagttggta
    15661 gagcaacgca ctcgtaacgc gtaggtcgta ggttcgatcc ctatagctgg cattaaaaga
    15721 gaagacagat atatcaagga tttttaatcc attgatacat ctgtcttttt gtttaaagga
    15781 aataaagtat gctttcttag caatagagca tgtattattt ctataaaaat ttaggagaat
    15841 aaaacgacta gcaatatagc tgtgaaatca aatatcacat gaactgccca acttgtaatg
    15901 atcgagttgc ttttaatggc agcttggttg aataacaatc ttgccacccc ttgtatcaaa
    15961 aggatgtgtg ctacagtgtg taaaatattt ccattatcat aagtactgta atggataaat
    16021 ccaaagatga ttgctgacaa gaaactagcg acaggaatgc tgaacttact agaaaaaata
    16081 gcgagaaaat acactgagaa caattcttct ccaagtatca tgataggcaa aataataaat
    16141 aataacgggc tgaaagcctc atttattgga ttgcctgtta aaccccattt caaaatttgt
    16201 tgcaaaaata ccgagactgc ggcactgatt actacattga taaaaaaata tttggctata
    16261 tttttgacag gagcgacagg tttcgagaac atttttttcg tatctttttt accaaaaaca
    16321 agtgtaaatg ctataaatcc agtgatagtt aaagaaaaaa tgctaataat ggatggcgca
    16381 aatagcaacc ctaacaaacc gagtacaacg acaatagtgc ccaatatccc ttttttaagt
    16441 tctatttttt ttcctaaaat gttattcatg ataataactc ctactattca g
//
LOCUS       DACDKM010000060        16373 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20834_2016
            SAMN12640370-rid9891663.denovo.060, whole genome shotgun sequence.
ACCESSION   DACDKM010000060 DACDKM010000000
VERSION     DACDKM010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640370
            Sequence Read Archive: SRR10040891
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16373)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16373)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742835.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742835.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:04:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,012
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 29 of 75
            Pseudo Genes (incomplete)         :: 33 of 75
            Pseudo Genes (internal stop)      :: 19 of 75
            Pseudo Genes (multiple problems)  :: 5 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16373
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640370-rid9891663.denovo.060"
                     /isolate="E20834_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            4..552
                     /gene="hslV"
                     /locus_tag="IV334_001744"
     CDS             4..552
                     /gene="hslV"
                     /locus_tag="IV334_001744"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002357489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HslU--HslV peptidase proteolytic subunit"
                     /protein_id="HAP8943810.1"
                     /translation="MVESPFHSTTICAVEKDGKFAMAGDGQVTMGESVVMKGTAKKVR
                     RIYNGEVVVGFAGSVADAFTLEEKFEGKLNEYNGNLQRAAVELAQEWRTQQSMQKLEA
                     MLIVMNDKEMLLVSGTGEVITPDDGILAIGSGGNFALAAARAMKNYAQNDMSAKEIAE
                     NALNIAADICIFTNHNIIVEEL"
     gene            566..1966
                     /gene="hslU"
                     /locus_tag="IV334_001745"
     CDS             566..1966
                     /gene="hslU"
                     /locus_tag="IV334_001745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATPase subunit HslU"
                     /protein_id="HAP8943811.1"
                     /translation="MYELKQTPRQIVSELDEYIVGQHAAKKSVAVALRNRYRRLQLDE
                     KMQQDITPKNMLMIGPTGVGKTEIARRLAKIVNAPFVKVEATKFTEVGYVGRDVESMV
                     RDLVENAIQIVEKEQYSRVYTSAKKNAEKRLVKILVPGIKKEQKQSTNQFEQMMNLFN
                     ASQQNTAEPQEEVTEDIRINRRTVLEQLQKGLLDSREVTIEVEDQKKPAPMMNNGLEQ
                     MGIDLNETLGALTPKKKVERTVTVKEALELLIKEESAKLVKDADIHSEAIKLAEESGI
                     IFIDEIDKITSKSQQSGEVSREGVQRDILPIVEGSQVNTKYGIIQTDHVLFIASGAFH
                     LSKPSDLIPELQGRFPIRVELDDLTAEDFVKILTEPNNALVKQYVALIGTENVSVTFT
                     KEAIERIAQIAFDVNRDTDNIGARRLHTILEKLLEDLLFEAPDMQMGEITITEAYVDD
                     KLGSIAANEDLSRYIL"
     gene            1984..2775
                     /gene="codY"
                     /locus_tag="IV334_001746"
     CDS             1984..2775
                     /gene="codY"
                     /locus_tag="IV334_001746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-sensing pleiotropic transcriptional
                     regulator CodY"
                     /protein_id="HAP8943812.1"
                     /translation="MTDLLTKTRKINTLLQQKNTFDMQADLPYDKMAVTLGGILDSNT
                     YIIGSDGVLLGYNERHDVNNERIKTMFVEKQFPESYTDTVAQLMKTEANIPITSDITA
                     FPVESREKYPFGLTTVVPIFGAGERLGTIILSRMEQSFADDDLVLAEYGATVVGMQIL
                     YQKSRSIEADVRSATAVQMAINTLSYSELKAVQAIFEALDGDEGRLTASNIADSIGIT
                     RSVIVNALRKLESAGIIESRSLGMKGTYLKVLNSRFKDELAKHSY"
     gene            2857..3732
                     /locus_tag="IV334_001747"
     CDS             2857..3732
                     /locus_tag="IV334_001747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase family protein"
                     /protein_id="HAP8943813.1"
                     /translation="MTVIIQNDQLIAEIAEHGAELISLKSKEHGLEYIWQGDPAYWGR
                     HAPVLFPFVGRLKDDQYVYQGKTYQMGQHGFARDMDFEVIEHGQEMASFILRSTPETL
                     EKYPFDFELVISYELGGDGITVHYQVANTGEKEMFFSIGGHPAFNVPLEEGLSFEDFY
                     LAFSPLKSRLRLPLKGPFIDMDQKTLGQTNTNLALNRELFEQDALVFETRGLNAYAIR
                     SEKSSHSVTLSYKDMPYVGIWSPYPKEAPFVCIEPWCGIADTVNSSGNLMEKVGINQL
                     GAHEIFKTKYSITVK"
     gene            complement(3864..4502)
                     /gene="plsY"
                     /locus_tag="IV334_001748"
     CDS             complement(3864..4502)
                     /gene="plsY"
                     /locus_tag="IV334_001748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HAP8943814.1"
                     /translation="MKLILLLILAYLLGSIPSGVWIGKLFFKKDIRQHGSGNTGTTNT
                     FRVLGKTAGIVVLLMDILKGTLATCLPMIFHLSGINPLWFGVCAILGHTFPIFAGFKG
                     GKAVATSAGMLLGFNPVFFVYSCIIFIVSLFCTSMVSLSSMISAVLITLSTIVLPYVA
                     PVILAKPNWLLTIIAFLVSSFIFYRHKDNIKRIRNHTESRIPFGLNSSKKKS"
     gene            4629..5063
                     /locus_tag="IV334_001749"
     CDS             4629..5063
                     /locus_tag="IV334_001749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HAP8943815.1"
                     /translation="MSVKNPTQETIFNYLEEAKNIAVVGLSNKPERTSYMVAQVLQDN
                     GYKIIPVNPLLAGEEILGEKVYANLTEIDDPIDIVDVFRRSEFLPEIAGDFLKTDAKV
                     FWAQLGIENKEAAEMLRKAGRTDIVMDRCIKIELGKLKENQK"
     gene            5229..7274
                     /gene="parE"
                     /locus_tag="IV334_001750"
     CDS             5229..7274
                     /gene="parE"
                     /locus_tag="IV334_001750"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HAP8943816.1"
                     /translation="MAKKVNNEYNDSSIQVLEGLEAVRKRPGMYIGSTDSRGLHHLVY
                     EIVDNAVDEALSGYGSEIDVTIHEDNSITVADSGRGMPVGMHASGIPTVEVIFTVLHA
                     GGKFGQGGYKTSGGLHGVGASVVNALSKWLIVTIVRDGVEYQQKFKNGGKPDGTLKKI
                     GKTKKANGTTVHFLPDDTIFSTTKFSYEILAERLRESAFLLKGVKISLSDLRGEEPVK
                     EIFHYEEGIKEFVDYLNEEKDTLTPVVYFSGEKEGIEVEVAYQYNDGYSENVLSFVNN
                     VRTKDGGTHEAGMKAAMTKSYNEYARKVGLLKERDKNLEGSDFREGLAAVLSIRVPEN
                     LLQFEGQTKEKLGTPVARTVVDNVISEQMGFYLQENSEMSQMLVRKAIKAREAREAAR
                     KAREESRNGKKRKKGESLLSGKLTPAQSRNPKKNELYLVEGDSAGGSAKQGRDRKFQA
                     ILPLRGKVINTEKAKMQDILKNEEINTMIYTIGAGVGPEFSIEDCNYDKVIIMTDADT
                     DGAHIQVLLLTFFYRYMKPLIEAGKVYIALPPLYKVSKGQGKKQVIEYAWTDDELAAM
                     IKKVGKGYMLQRYKGLGEMNAEQLWETTMDPTSRTLIRVRIDDAAQAERRVTTLMGDK
                     VEPRRKWIENHVQFTLEEDGSILDKKEDTEISPSVSNDLLDEERADKNENNQLFEVE"
     gene            7286..9736
                     /gene="parC"
                     /locus_tag="IV334_001751"
     CDS             7286..9736
                     /gene="parC"
                     /locus_tag="IV334_001751"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HAP8943817.1"
                     /translation="MENRQEVQELTLEEVMGDRFGRYSKYIIQERALPDIRDGLKPVQ
                     RRILFAMNKDGNTYDKGFRKSAKSVGNIMGNYHPHGDISIYEAMVRMSQDWKLREVLI
                     EMHGNNGSMDGDPPAAMRYTEARLSKLSGEMLADIEKETVDLVWNFDDTEKEPTVLPA
                     RYPNLLVNGSTGISAGYATEIPTHNLAEVIDGTIYMIDHPQASLEKLMEYIPGPDFPT
                     GGILQGKDEIKKAYETGRGKVILRSKTKIEAMKGNKQQIVITEIPYEVNKATLVKKMD
                     EIRLNKKIDGIAEVRDESDRTGLQIVVELKKDANAQGILNYLFKNTELQINYNFNMVA
                     IDHMTPHQVGLKDILRSYIEHRKQVITKRSQFDLAKAQKRQHIVEGLMKALSILDEVI
                     ATIRESKDKKDAKKNLVDVFQFTEEQAEAIVTLQLYRLTNTDITELQKESESLIAQIT
                     ELNKILSNDKELFSVMKKELREVKKNYSSARLTTIEDEIEEIKIDTQVLVAQEDVIVS
                     VTREGYVKRTSLRSYSASKPEEIGMREGDYLLYSGELSTLDHVLLITNKANVIYRPVH
                     ELPDLKWKDAGEHISQTISNLSVDESILAVFPYQKIQAEKTFVFISKNGLIKQTRMTE
                     FEPWRTYKSRPLSCMKLKAEEDELIAVYLKEDQPGLDVFLVTHQGMGLRYPLTDVPVV
                     GAKAAGVKSMNLKDGDRIINGLLVLAEGDTPIVIVTQRGAVKRMLAQEISQTSRAKRG
                     VTVMRELKKNPHRIIYMSEGRSKEITLMNQKGQQLTIDPTDFPIGDRTSNGSFAMDEK
                     KGGEVIEVIDAPEIFIES"
     gene            10013..12244
                     /gene="pflB"
                     /locus_tag="IV334_001752"
     CDS             10013..12244
                     /gene="pflB"
                     /locus_tag="IV334_001752"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HAP8943818.1"
                     /translation="MEQWNGFKGTKWKEGVDTRDFIQNNYTEYRGDDSFLEGPTEATE
                     KLWDELQKLNDIQFERNGVYDMDTEIVSTITSHDAGYLDKDLEKIVGLQTDKPLKQAF
                     MPFGGINMANNALKSNGYTPNDELTHIFTDWRKTHNQGVFDAYTAEMRAARKNKIITG
                     LPDAYGRGRIIGDYRRVALYGIDYLMEQKKKDHDMTGYHTMSEEVIRLREEITEQYRA
                     LAQMKEMAAKYGYDISKPAANAKEAVQWLYFGYLAAIKSQNGAAMSIGRISAFLDIYI
                     QRDLDNGVITEKEAQELIDHLVMKLRMVKFARTPEYNQLFSGFPIWATLSIAGMGLDG
                     RSLVTKNDFRLLHTLTNMGPSPEPNLTVLYSEHLPEGFRTYASRIAIESSSIQFENDN
                     QLREQWGSDDCAIACCVSGTVVGKDMQFFGARANLAKTLLYAINGGVDEVTGAQVGPE
                     YRPITSEILDFEEVKHNFMNMMDWLAELYVNTLNIIHYMHDKYSYEAAQLALMDSDLQ
                     RTFATGIAGISHAADSLSAIKYAKVKAIRNEKGIAVDFEVDGDFPKYGNDDDRADELV
                     SWILEYFMGQIKRHKTYRNSKPTTSLLTITSNVVYGKNTGNTPDGREAGKPLAPGANP
                     SYNAEQSGLLASLNSTAKMNYGCALDGISNTQTINPSGLGKEQDTRIENLRNVLDGYF
                     SKGAYHLNVNVFSNDLLRDAMENPMKYPNLTIRVSGYAVKFRDLTREQQLDVLMRTSH
                     DRM"
     gene            12488..13249
                     /gene="pflA"
                     /locus_tag="IV334_001753"
     CDS             12488..13249
                     /gene="pflA"
                     /locus_tag="IV334_001753"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate lyase-activating protein"
                     /protein_id="HAP8943819.1"
                     /translation="MEEKTIGYVHSIETFGSVDGPGLRFVVFMQGCRMRCQFCHNPDT
                     WNIGGGKEYTADELLDKAERFRPYWGDKGGITVSGGEPLLQIDFLIELFKKAKERKMH
                     TTLDTCGKPFTYEEPFFSRFQELMKYTDLLLFDIKHIDNEEHKKLTHWDNENILEMAQ
                     YLSKINKPVWIRHVLVPERSDYDEYLIRLDNFIQTLSNVDRVEILPYHTMGKYKWETL
                     GLKYPLEGIEPPTKERVENAKRLLHTEEYTKYLTR"
     gene            13334..14260
                     /locus_tag="IV334_001754"
     CDS             13334..14260
                     /locus_tag="IV334_001754"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese-dependent inorganic pyrophosphatase"
                     /protein_id="HAP8943820.1"
                     /translation="MSKILVFGHQNPDTDAIGAAIAFSHLQNELGKETEAVALGTPNE
                     ETQYALDHFQLSAPRVVESAKAENADVMLVDHNEFQQSISDISEVNILAVVDHHRIAN
                     FETANPLYYRAEPVGCTSTIILKLYKENQVTIPKEIAGIMLSAIISDTLLFKSPTCTP
                     EDEAAAKELAAIAEVDLNTYGLEMLKAGTNLSDKSAAVLLDLDAKSFPMGSSNVRIAQ
                     VNTVDLNEVMDRQAELEAAMEDEMKANGYDLFVLVVTNILDSDSELLVAGKGKEKVEE
                     AFQTTLTNNRALLKGVVSRKKQVVPQLTAVFS"
     gene            14442..15335
                     /locus_tag="IV334_001755"
     CDS             14442..15335
                     /locus_tag="IV334_001755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1803 domain-containing protein"
                     /protein_id="HAP8943821.1"
                     /translation="MNYYSKNKENFYQKLTDDPLFSSLTDYLYEHREQETILRELKKE
                     FSQNKFSHFLDLLIDAGLIKREERRYHLNFPIFDPKDYLQQATSAAETIAEQLKRLSV
                     DEQKLAMGEVIWAYCFEDERKEAYFYGVRNSRETELLRTTAGNEKYRFITLSSIEHFP
                     LTLANYFFVQKNQLPVTKAFKELAELIGDVNESYFFDQIEVIVDRIRKNKYKNRRPSI
                     FHQSLLVTNTIKEEESFTLELPIVEKNNFEIELPTLDPSLTMEEMAFLKRQIFSELSK
                     KFIPHAFSYIKEYGTVLVSKT"
     gene            15469..15594
                     /locus_tag="IV334_001756"
     CDS             15469..15594
                     /locus_tag="IV334_001756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP8943822.1"
                     /translation="MQKNGEKCGMTKEVVIRKVRFLNNQYYDSVKYGILWEELAD"
     gene            complement(15728..16360)
                     /locus_tag="IV334_001757"
     CDS             complement(15728..16360)
                     /locus_tag="IV334_001757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8943823.1"
                     /translation="MEIQRRTTNFLHLGSFFATLTILFFLCYSYLKTSHAPSSALGWL
                     LLDHSDITEAEENPFFLILSSLCGLLFSVYFGAYFFHRHPGTLNEASSVLIAFKTFSF
                     LAILFYSFNHNTLFFFIMNGLVVILSIFTYFIFSLIFSQLKCPLFFRLVPVTCFIYSL
                     FSFFVLALMPLTNPSTIQVAGNLLDMLFIVSLGVFMWQRKNKQNKKHFEA"
BASE COUNT         5815 a         2791 c         3492 g         4275 t
ORIGIN      
        1 aacatggtcg aatcaccatt tcattcaacc actatctgtg ccgtcgaaaa agacgggaaa
       61 tttgctatgg ctggagatgg ccaagtaaca atgggtgaat ctgttgtcat gaaaggcact
      121 gcaaagaaag tacgacgtat ctacaatgga gaagttgttg taggtttcgc cggaagtgtt
      181 gcagatgcgt ttactttaga agaaaaattt gaaggcaaac taaacgaata taacgggaat
      241 ctacaacgcg cagctgtcga attagctcaa gaatggcgga cacagcaatc tatgcaaaaa
      301 cttgaagcga tgctgatcgt aatgaatgat aaagaaatgc ttcttgtttc aggtacagga
      361 gaagttatca caccagatga tggtatctta gctatcggat caggagggaa ttttgcgttg
      421 gctgcagccc gagcaatgaa aaactatgct caaaacgaca tgagtgcgaa agaaattgca
      481 gaaaatgcgt taaatattgc cgcagatatc tgtatcttta ctaatcataa tattatcgta
      541 gaagaactgt aagaggtgag tagatatgta tgaattaaaa caaacaccaa gacaaatcgt
      601 aagtgaatta gatgaatata tcgtaggaca gcacgcagca aaaaaatctg ttgccgttgc
      661 tttgcgtaac cgctatcgcc gtttgcagtt agatgaaaaa atgcaacaag atattacacc
      721 aaaaaatatg ctgatgatcg gacctacagg ggtcggtaaa acagaaatcg cacgacgttt
      781 ggcaaaaatc gtcaatgcac catttgtcaa agtcgaagca acaaaattta ctgaagtcgg
      841 ttatgtcgga agagatgtag aatcaatggt tcgggattta gtagaaaatg cgatccaaat
      901 cgtagaaaaa gagcagtaca gccgtgtata cacaagtgct aaaaaaaatg cagaaaaacg
      961 tttggtaaaa atcttggtac ctggtatcaa aaaagaacag aaacaatcca ctaatcaatt
     1021 tgaacaaatg atgaatcttt ttaatgctag tcagcaaaat accgctgaac cgcaagaaga
     1081 agtgacagaa gatattcgaa tcaatcgccg tacagtcttg gaacaactac aaaagggttt
     1141 actggatagt cgtgaagtaa ccattgaagt tgaagaccaa aagaaacctg caccaatgat
     1201 gaacaatgga ttggagcaaa tggggatcga cctaaatgaa actctaggtg ctttgacacc
     1261 gaagaaaaaa gtggaacgga cggtaacagt caaagaagct ttagagttat tgatcaagga
     1321 agaatcagca aaattagtca aggatgctga tatccatagt gaggcaatca aattagcgga
     1381 agaaagtgga attattttta tcgatgaaat cgataagatc acttctaaat cacagcaatc
     1441 tggagaagta tccagagaag gcgtccaaag agacatattg ccaattgtcg aaggttcaca
     1501 agtaaacaca aaatacggga tcatccaaac agatcacgtc ctattcattg cttcaggagc
     1561 tttccatttg tctaaaccaa gtgatctaat tccagaactt caaggacgct tcccgatacg
     1621 ggtagaatta gatgatttga cagctgaaga ttttgtgaag attctgaccg aaccaaataa
     1681 tgctttagta aaacaatacg tcgcgttgat cggtacagaa aatgtgtcag ttacgtttac
     1741 aaaagaagca attgaacgga tcgctcaaat cgctttcgat gtcaaccgtg acacagataa
     1801 tatcggcgca cgcagactgc ataccatttt ggaaaaacta ctagaagatc tattatttga
     1861 agcaccagat atgcaaatgg gagaaataac gatcacagaa gcatatgtgg atgacaaact
     1921 tggctcaatt gcggcaaatg aagatttaag tcgctatatt ttataaaaaa tcggaggacg
     1981 acaatgacag acttgctaac gaaaacccgc aagataaaca cacttttgca gcaaaagaat
     2041 acttttgaca tgcaagcaga tttgccttac gataaaatgg ctgtcacact aggtggtatt
     2101 cttgacagca acacctatat cataggaagt gacggagtat tactaggtta taatgaacgt
     2161 cacgatgtga acaatgaacg aatcaaaaca atgtttgttg agaaacaatt tcctgaatct
     2221 tatactgata cagtggcaca gttgatgaaa actgaagcta atatcccaat tactagtgat
     2281 attactgcat ttccggtaga gtcaagagag aaatatccat ttggtctgac gactgtcgtt
     2341 ccgatctttg gagctggcga acgtttgggt acgattatct tatctcgtat ggaacaatcg
     2401 tttgcagatg acgatctagt cttagcagaa tatggagcta cggttgtcgg tatgcagatc
     2461 ctttatcaga agtctcgaag catcgaagca gatgtacgga gtgcaacggc tgtccaaatg
     2521 gcaatcaaca cattatctta tagcgaattg aaggctgtac aagctatctt tgaagcattg
     2581 gatggagatg aaggtcgtct gactgcttct aatattgccg atagtatcgg catcactcgt
     2641 tctgtgatcg tcaatgcttt acgaaaattg gaatcagctg gaatcattga atctcgttcg
     2701 ttaggcatga agggaactta tttaaaagta ttgaattccc gttttaaaga cgagttagca
     2761 aaacacagtt attagaagta aggggactgg ggcagataga aatgtttgtt ccagccttcc
     2821 cattttaaaa agtaagtcga agttggagga atggcaatga ctgttattat tcaaaatgac
     2881 cagctgatcg ccgaaatcgc tgaacacggt gcggaattga tcagtctgaa atcaaaagaa
     2941 catggtttag agtatatctg gcaaggtgat ccagcgtatt ggggcagaca tgcaccggtt
     3001 ttattccctt ttgttggacg tttaaaagat gatcagtatg tttatcaagg gaaaacatat
     3061 caaatggggc aacacggatt tgctcgggat atggattttg aagtcattga acatggacaa
     3121 gaaatggcta gtttcatctt aaggagtacg ccggagacat tagaaaagta tccatttgat
     3181 tttgaattag tgatcagcta tgaattagga ggagacggca tcacggttca ttatcaagtg
     3241 gcaaataccg gtgaaaaaga aatgttcttt tctatcggtg gacatcctgc tttcaatgta
     3301 cctcttgaag aaggattgtc atttgaagat ttctatctgg cattttctcc attgaaatca
     3361 agattacgcc taccattgaa aggcccattc atcgatatgg atcaaaagac gttaggtcag
     3421 acaaatacga atctggcttt gaatcgtgaa ctttttgagc aagatgcctt ggtttttgaa
     3481 acaaggggtc taaatgcgta cgccattcga agtgaaaaat cttcccatag cgtaacttta
     3541 agctataaag atatgcctta tgtagggatc tggtctcctt atccaaaaga agcgcctttc
     3601 gtttgtatcg aaccgtggtg cggaatcgct gacacggtga acagtagtgg aaacttgatg
     3661 gaaaaagtag ggatcaatca actaggtgcc catgaaatct tcaagacaaa atattccatc
     3721 acagtgaagt aagaggttgt gaccgagaag ttagcaggga acaccgttta ccaaggtgtg
     3781 taaaaaagct atcctgtaag taataaggga gtgtgacatc gatttgtcac actcccgttt
     3841 tatctctttg gaatgaaaaa gtatcaggat tttttcttgc tgctattcaa accaaacggg
     3901 attcgacttt cagtatggtt tctgatacgc ttgatattgt ctttatgccg ataaaagata
     3961 aaggaagaaa ctaaaaaggc aatgatcgtc agtaaccaat tgggcttagc caaaatcaca
     4021 ggagctacat aaggaaggac gatcgtagat aaagtgatca atactgcact gatcatactg
     4081 ctcaaactca ccatacttgt acaaaataaa ctaacgataa agatgataca agaataaaca
     4141 aaaaatacag gattaaatcc taaaagcatt cctgcacttg tcgcaacagc ttttcctcct
     4201 ttgaacccag caaaaatcgg aaatgtatgg ccaagaatgg cgcatacccc aaaccaaaga
     4261 gggttaatcc cagaaaggtg gaagatcatc ggcaaacaag tagccaatgt accttttaaa
     4321 atatccataa gcaggacaac aatcccggct gttttgccta atacacgaaa tgtattggtc
     4381 gttccagtat taccgctccc gtgttgtcgg atatcttttt tgaaaaacaa tttgccaatc
     4441 cagacaccgg aaggaataga acctaaaaga tatgctaaaa taagtaataa gatcaatttc
     4501 attttttacc ctctttcaag ttgaaaacta tcctttattt tagcatgaat aggtaacagg
     4561 aacaattgaa aaaatgaaag atacaagtat aatagatgga ataaaaagaa aagcaagggg
     4621 tggcagaaat gtctgtaaaa aatccaacac aagaaacgat tttcaactat ttggaagaag
     4681 caaaaaatat tgccgtcgta ggcttgagca ataaaccaga acgaacaagt tacatggttg
     4741 cacaggtatt gcaagacaat ggatataaaa tcataccagt aaatccatta ttggctggtg
     4801 aagaaattct aggcgagaaa gtctatgcga atttgacaga aatcgatgat ccaatcgata
     4861 tcgtagatgt atttagacgg agtgaatttt tacctgaaat agcaggtgat tttttgaaaa
     4921 ctgatgctaa agtgttctgg gcacaattag ggattgaaaa caaagaagca gctgagatgc
     4981 taagaaaagc aggacgtaca gatatcgtaa tggatcgttg tattaagatt gaacttggta
     5041 agttgaaaga aaaccaaaaa taaaaatgac cacaaagaag agatgatcga tagaggggaa
     5101 tgtcgaaaaa gcggttagga gataattaaa attgatttct cgtctaccct cttttttttc
     5161 acgacctttt ctagtatgat aatcgtggtg cggttttgcc gcaaagtatt attgaaggag
     5221 cgtttgcctt ggcaaaaaaa gtaaacaacg aatacaatga ctcgtccatc caagtattgg
     5281 aaggattaga ggcagttaga aaaagaccag ggatgtacat tggatccact gacagccgag
     5341 gcttgcatca tttagtctat gaaatcgtcg ataatgcagt tgacgaagca ttatcaggtt
     5401 atggcagtga gattgatgtg accatccatg aagacaatag tataactgtt gcagatagcg
     5461 gacgtgggat gcctgtagga atgcatgctt ccggaatacc aacagttgaa gtgattttta
     5521 ctgtgctgca tgctggcgga aagtttggtc aaggagggta caaaacttct ggtggactgc
     5581 acggtgtagg agccagtgtg gtaaatgcct tatcgaaatg gctaattgta acgatcgtta
     5641 gagatggcgt agagtatcag cagaaattta aaaacggcgg taaacctgac ggtactttga
     5701 aaaagatagg aaaaacaaaa aaagcaaacg ggacgactgt ccatttttta ccagatgata
     5761 caatattcag tacaacgaaa ttttcatatg aaattttagc cgaacggtta agagaatctg
     5821 cctttcttct aaaaggcgtt aaaatctctc tttctgatct tcgtggagaa gaaccagtca
     5881 aggaaatttt tcattacgaa gaaggaatca aagaatttgt tgattatctg aatgaagaaa
     5941 aagatacatt gacaccagtt gtttattttt caggagaaaa agaaggtatc gaagtagagg
     6001 tcgcttatca atacaatgac ggttattcag aaaatgtttt atcttttgtc aataacgttc
     6061 ggacaaaaga tgggggaacc catgaagctg gtatgaaagc ggcgatgacg aaatcttata
     6121 atgaatatgc gagaaaagtt ggtttgctga aagaacggga caagaatctg gaaggtagtg
     6181 atttcagaga agggttagct gctgtacttt ctatccgtgt acctgaaaat cttctgcaat
     6241 ttgaagggca aacaaaagaa aaacttggta cgccagttgc tcgtaccgta gtagataacg
     6301 tcatcagtga acaaatgggc ttttatttgc aggaaaatag cgaaatgagc cagatgttgg
     6361 tccgtaaagc aatcaaagcc agagaagcga gagaagcagc acgtaaagcc agagaagaaa
     6421 gccgaaatgg gaagaagcgt aaaaaaggcg aatctcttct ttcaggaaaa ttgacacccg
     6481 cacaatcccg aaaccctaag aaaaatgaat tgtatttagt cgaaggggat tctgctggcg
     6541 gatctgctaa acaaggaaga gacagaaagt tccaagcaat tctaccttta cgaggaaaag
     6601 tcatcaatac cgaaaaagct aaaatgcaag atatcttgaa aaatgaagaa atcaatacga
     6661 tgatctatac gattggtgca ggagtcggtc ctgagttttc tatcgaagac tgtaattacg
     6721 acaaagtaat catcatgacc gatgcggata cagacggcgc ccatattcaa gtattactgc
     6781 ttactttctt ttatcgttat atgaaaccgc tgattgaagc tgggaaagta tatatcgcgt
     6841 taccgccttt atataaagta tccaaaggac aaggaaagaa gcaagtcatt gaatatgcgt
     6901 ggacagatga cgaattggca gcaatgatta aaaaagtcgg caaaggatat atgctgcagc
     6961 gatataaagg acttggagag atgaatgcgg aacaattgtg ggaaacaacg atggatccga
     7021 cttcccgtac gttgatccgc gtccgtatcg atgatgctgc gcaagctgaa cgtcgagtaa
     7081 ccacactgat gggtgacaaa gtagaacctc gaagaaaatg gatcgaaaat cacgtgcaat
     7141 ttacgctcga agaagatggc agtatcttag ataaaaaaga ggatactgaa atctctcctt
     7201 cagtttctaa tgatttatta gatgaagaac gagcagataa aaatgaaaac aatcagttat
     7261 ttgaagtaga ataggaggtc acctgatgga aaatcgacaa gaagtacaag aattaacttt
     7321 agaagaagtg atgggtgacc gcttcggacg ttattccaaa tatattatcc aagaacgtgc
     7381 actgcctgat atccgtgatg gattgaagcc agtgcaacgc cggatattat ttgccatgaa
     7441 caaagatggc aatacgtatg acaaaggatt ccgtaaatca gcaaaatctg tcggaaatat
     7501 catggggaat tatcatcctc atggtgacat cagtatttat gaagcgatgg tccgcatgag
     7561 ccaagactgg aagttgcggg aagtactgat cgaaatgcac gggaataacg ggagtatgga
     7621 cggtgatcca cctgctgcga tgcgttatac tgaagccaga ttatccaagt taagtggaga
     7681 gatgctagca gatatcgaga aagaaacagt cgatctcgtg tggaattttg atgatacaga
     7741 aaaagaacca acagtccttc cagctcgata cccaaacctt ctcgtcaatg gatcgacagg
     7801 tatatctgct ggatatgcaa cagaaatccc gactcataac ttagctgaag tgatcgatgg
     7861 cacaatctat atgatcgatc atccacaagc gagccttgaa aaattgatgg aatatattcc
     7921 tggacctgac tttccaacag gagggatctt gcaaggaaaa gatgaaatca aaaaagctta
     7981 tgaaacagga cgaggaaaag ttattcttcg ttcgaaaaca aagatagagg caatgaaggg
     8041 taataaacag caaatcgtga tcacagaaat cccatatgaa gtaaataaag cgaccttggt
     8101 gaaaaaaatg gatgagatcc gtttgaataa gaaaatcgat ggaatagctg aagtacgaga
     8161 tgaaagtgat agaacaggtc tgcaaattgt cgtcgaattg aaaaaagacg ccaatgctca
     8221 aggaattttg aattatttgt tcaaaaacac tgagctacag atcaattaca atttcaatat
     8281 ggtcgctatc gaccatatga ctccgcatca agttggactg aaagacattt taagaagtta
     8341 tatcgagcat cgaaagcaag tcataacaaa acgtagtcaa ttcgatttag ccaaagctca
     8401 aaaaagacag catatcgtag aaggattgat gaaagcttta tcgattctgg atgaagtaat
     8461 cgcaacgatt cgtgaaagta aagacaaaaa agacgccaaa aagaatttag tcgacgtctt
     8521 tcaatttaca gaagaacaag cagaagcaat tgtcacattg caattatacc gtttgacgaa
     8581 cacggatatc acagagcttc aaaaggaatc cgaaagcttg attgcgcaaa tcacagagtt
     8641 gaataaaatc ctttcaaatg acaaagagct tttctctgtt atgaaaaaag aattgcgaga
     8701 agtgaaaaaa aattattctt ccgctcgtct aacgacaatc gaagacgaaa tcgaagaaat
     8761 caaaattgat acgcaagtct tagtggcaca agaagacgta attgtcagtg tgacgcgaga
     8821 aggctatgtg aagcgaacaa gccttcgttc ttatagtgct tccaaaccag aagaaatcgg
     8881 gatgcgagaa ggcgactact tgctatactc aggtgaattg agtacattgg accatgttct
     8941 tctgatcaca aataaagcaa atgtgatcta ccgccctgtc catgaactac ccgatttgaa
     9001 atggaaagat gctggtgagc acatttctca aacaatctcc aatctgtctg tagacgaatc
     9061 gattttagct gttttcccat atcaaaaaat tcaagcagag aaaacatttg tattcatcag
     9121 caagaatgga ttgatcaagc aaacacgaat gacagagttc gaaccatggc gcacctataa
     9181 gagccgtccg ctcagctgta tgaagctaaa ggcggaagaa gatgaactga ttgctgtgta
     9241 tctgaaagaa gatcagccag gcttagatgt tttcttagta acgcatcaag gaatgggttt
     9301 gcgctatcct ttaacagatg ttcccgttgt tggagcaaag gcagctggag tgaagtctat
     9361 gaatttgaaa gatggagata ggattatcaa cgggctgctt gtgttagcag aaggtgatac
     9421 gccgatcgta atcgttactc agcgaggagc tgttaaacgc atgcttgctc aagaaatcag
     9481 ccaaacttca cgtgcgaagc ggggagtgac ggtcatgcgt gagctgaaaa aaaatccgca
     9541 ccgtattatc tatatgtcgg aaggaagatc aaaagaaata accttgatga atcaaaaagg
     9601 gcagcagtta acgattgacc caactgattt ccctataggt gatcgaacat cgaatggatc
     9661 atttgcgatg gatgagaaaa aaggtggaga agtcatcgaa gttatcgatg ctcctgagat
     9721 tttcatcgag tcgtaactgt tgtagtattt taatgaagag acgccatctg tattaataca
     9781 ggtggcgtct ctttttcatt catttttaat aaatccttat acgtttgcaa aaaaagatga
     9841 aagtgttcac aatgcttaaa tgttggtatg tcaacgttga tttaaatata tgtaaataaa
     9901 acataaaaat tgttaaataa aaaacaagaa aacccttgca aagatagggt gctgtgttat
     9961 actaatcacg taaaaactgt tacataaaac agaaatttag gaggtccata caatggagca
    10021 atggaatgga tttaaaggta ccaaatggaa agaaggcgta gacactagag actttattca
    10081 aaataactac actgaatatc gcggtgatga cagctttttg gaaggcccaa cagaagcaac
    10141 agaaaaatta tgggatgaat tgcaaaagtt aaatgatatc caattcgagc gtaacggtgt
    10201 ctatgatatg gacacagaaa tcgtatcaac tattacttca cacgatgcag gctatttgga
    10261 taaagatttg gaaaaaatcg tcggtctaca gacagacaaa cctttgaaac aagcattcat
    10321 gccgttcggc ggaatcaata tggctaacaa tgcgctaaaa agcaatggct atacaccaaa
    10381 cgatgaattg acacatattt tcactgactg gcgcaaaaca cataatcaag gtgtatttga
    10441 cgcttatact gctgaaatgc gtgctgctcg taaaaacaaa atcattacag ggcttccaga
    10501 cgcttacggt cgcggacgta tcatcggtga ctatcgtcgt gtggcattgt atggaatcga
    10561 ctatttgatg gaacaaaaga aaaaagatca tgatatgact ggttatcata caatgagtga
    10621 agaagtgatt cgtttacgtg aagaaatcac tgaacaatat cgtgcattag cgcaaatgaa
    10681 agaaatggct gcgaaatatg gctatgatat ttctaaacca gctgcaaatg caaaagaagc
    10741 tgtccaatgg ctatacttcg gatatttagc agcaatcaaa tcacaaaatg gtgctgcgat
    10801 gtcaatcgga cgtatctcag cattccttga tatctatatc caacgtgatt tggataatgg
    10861 agtaatcaca gaaaaagaag cacaagaatt gatcgaccac ttagtaatga aattacgtat
    10921 ggtcaaattc gcgcgtacac ctgaatataa ccaattattc tcaggattcc ctatctgggc
    10981 tactttatcc attgcaggta tgggattaga tggtcgttca ttagttacaa agaatgattt
    11041 ccgtctgctt catacattaa caaatatggg accttcacca gaaccaaact tgactgtttt
    11101 gtattctgaa caccttccag aaggattcag aacatatgct agcagaatcg ctatcgaaag
    11161 ttcttcgatt caattcgaaa atgataatca gcttcgtgaa caatggggtt ctgatgattg
    11221 tgcgattgct tgctgtgtat caggaacagt tgtcggaaaa gacatgcaat tctttggcgc
    11281 tcgtgctaac ttggctaaaa cactattata tgccatcaat ggcggagtcg atgaagtgac
    11341 tggcgcccaa gttggaccag aatatcgtcc aatcacatca gaaatactag actttgaaga
    11401 agtgaaacat aacttcatga atatgatgga ttggttagca gaactatatg tcaatacgtt
    11461 gaacatcatt cattatatgc atgataaata ttcttatgaa gcagctcagc tggctttgat
    11521 ggattctgac ttgcaacgca catttgctac aggtattgct gggatctctc atgcggcaga
    11581 cagcttgtca gcgatcaaat atgcaaaagt aaaagcaatc cgtaatgaaa aaggaatagc
    11641 agttgatttt gaagtggatg gcgacttccc taaatacgga aatgatgatg accgtgcaga
    11701 tgaattagtt tcatggatct tggaatactt catgggacaa atcaaacgtc ataaaactta
    11761 ccgcaactca aaaccaacaa cttcattatt gacgattact tcaaatgttg tttacggtaa
    11821 aaatacagga aatactcctg atggccgtga agctggaaaa ccacttgcgc caggagcaaa
    11881 tccaagttac aatgcggaac aaagtgggtt attagctagc ctgaactcaa cagctaaaat
    11941 gaattacggc tgtgcattgg atggaatttc aaatacccaa acaatcaatc cttcaggtct
    12001 tggaaaagaa caagatacaa gaatcgaaaa cttgcgtaat gtcttagatg gatacttctc
    12061 aaaaggagct tatcatttga atgtcaatgt attcagtaat gatttattgc gtgatgcaat
    12121 ggaaaatcca atgaaatatc cgaacttgac gatccgggta tctggttatg cagttaaatt
    12181 ccgtgacttg acaagagaac aacaattaga tgtcttgatg agaacttcac acgacagaat
    12241 gtaaaaagtt cgtgaaaaaa gtttttcgac ttagcgtcga aaggacgttg aatagccatc
    12301 gcatcaaaat cgaataatca gtatgcttat ttgaaagaaa cgatttacag gggcaggact
    12361 tgtatgttcc gtccctgtaa attttacaca taacgaagcg gcaattgcga aaaattttgt
    12421 tcagaaagct tcgcttcgac agctaacttg tacttaaaat gaaatcaaag aaaagatgtg
    12481 aacttaaatg gaagaaaaaa caatcggcta tgttcattct atcgaaactt tcggttcagt
    12541 agacggacct ggactgcgct ttgtcgtgtt tatgcaagga tgccgcatgc gttgccaatt
    12601 ttgtcacaat ccagatactt ggaatattgg cggagggaaa gaatacacag cagatgaact
    12661 tttagataaa gcagaacgct tcagacctta ctggggagat aaaggtggta ttactgttag
    12721 tggtggagaa cctctattgc agatcgattt tttgatcgaa ctatttaaaa aagcaaaaga
    12781 gcggaagatg catactacat tggatacatg cggaaaacct tttacgtatg aagaaccttt
    12841 cttttcacgt tttcaagaat tgatgaagta tacagattta ctgttatttg acatcaaaca
    12901 catcgataat gaagagcata aaaaactaac gcattgggac aatgaaaata ttttagaaat
    12961 ggctcaatat ttgtcaaaaa tcaacaaacc ggtttggatt cgccatgtac tagttcctga
    13021 gcgaagtgat tatgatgaat atttgatccg actagacaac tttattcaaa ccttgtcaaa
    13081 tgtagacaga gtagagattc ttccttatca tacgatggga aaatacaagt gggaaacact
    13141 tggtttgaaa tatccattag aagggatcga accacctaca aaagaacgtg tcgaaaatgc
    13201 taaacgattg cttcatacag aagagtatac aaaatattta acgcgatagg tcgcatcgga
    13261 aagaagataa gtattgcttc tttccgtttt tttgctatta ttaaatagga gaaatcacaa
    13321 aggagcgaat gacatgtcaa aaattttagt ttttggtcac caaaatccag atacagatgc
    13381 aatcggggca gccattgcgt tttcacactt gcaaaatgaa ttaggtaaag aaacagaagc
    13441 agttgctcta ggtacaccta atgaagaaac acaatacgca ttagaccact ttcagctatc
    13501 tgcaccacga gtagtagagt cagctaaagc agaaaatgca gatgtcatgc ttgtggatca
    13561 taatgaattc caacaaagta tttcagatat cagcgaagta aatattttag ctgtagtaga
    13621 ccaccatcgt attgcaaact ttgaaacagc taatccattg tactatcgtg cagaaccagt
    13681 tggatgtaca agtacgatta ttttaaaact atataaagaa aaccaagtaa caattcctaa
    13741 agaaatcgca ggaatcatgc tctcagctat catttcagat acattgttgt tcaaatcacc
    13801 aacatgtacg cctgaagatg aagcagctgc aaaagaactt gcagcaatcg ctgaagtcga
    13861 tctaaacact tatggacttg aaatgttgaa agcaggaaca aatctaagtg ataaatcagc
    13921 tgcggttctt cttgatttgg atgcaaaaag tttcccaatg ggcagttcca acgttcgtat
    13981 cgctcaagtc aatacagtgg accttaatga agtgatggat cgacaagcag agctagaagc
    14041 agcaatggaa gatgaaatga aagcaaatgg ctacgatctg ttcgtattag ttgtcacaaa
    14101 catcttagac agtgattcag aattacttgt tgctggtaaa ggaaaagaaa aagtagaaga
    14161 agctttccaa acaactttga ccaacaaccg tgctttgctt aaaggtgtcg tttcacgtaa
    14221 aaaacaagtc gtacctcaat tgacggctgt atttagctaa tcaaatggta gtatgaagca
    14281 ttcaaaatga atggataagc gattgatata aaaagaaaca tagaacatac taaaggccga
    14341 agaaatggag gagaataaca gtcaattatg actcttgttc ttcctccttt ttacgtaaaa
    14401 gccttttaag taaaataaaa cggatagaaa agagaacata tatgaactat tattcaaaaa
    14461 ataaggaaaa cttttatcaa aaactgaccg atgatccgct tttttcttca ttgaccgatt
    14521 atctatatga acatcgagaa caagaaacga ttctaagaga attgaaaaaa gaattttcgc
    14581 aaaataaatt ttcccatttt cttgatttat tgatcgatgc aggattaatc aagagggaag
    14641 aacgaagata tcatttgaac tttccaatat ttgatcctaa agattacttg caacaagcca
    14701 cttcggctgc tgaaaccata gctgaacaat tgaagagact ctctgttgat gaacaaaaat
    14761 tagcgatggg agaagttatt tgggcgtatt gtttcgaaga tgaacggaaa gaagcgtatt
    14821 tttatggagt aagaaacagc cgagagacag aattgctgag gacaactgca ggaaatgaga
    14881 agtaccgttt tatcacactt tcctctatag aacacttccc gcttactttg gctaattact
    14941 tttttgtaca gaagaatcag cttcctgtaa cgaaggcatt caaagaatta gccgaactta
    15001 ttggagacgt aaatgaatcg tacttttttg atcaaataga agttatcgtt gacaggatac
    15061 gtaagaataa atataaaaat cgccggccta gtattttcca tcagtctcta cttgtgacga
    15121 atactataaa ggaagaagaa tcattcacat tagaattacc tattgtcgaa aaaaacaatt
    15181 ttgagattga attaccaaca ttggatcctt cattgacaat ggaggaaatg gcctttctta
    15241 aacgacaaat cttttctgag ctgagcaaga aatttatacc gcatgcattt tcttatatca
    15301 aagaatatgg aacagtttta gtgagtaaaa cctaagagag cagtaagcaa aaattatact
    15361 gactcttgca ggaggagcct ttctcttcct ttatggttaa aagaattatt tgagtatttt
    15421 gcctatctac cacctattag attcactaca tggtctggaa acaaaggaat gcaaaaaaat
    15481 ggtgagaaat gtggaatgac aaaggaagta gtgataagaa aagttagatt tttaaataat
    15541 caatattatg attcagtcaa gtatggcatt ttatgggaag aactagctga ttagattagg
    15601 aagtccattt gataatccag aatcactaag aaacgaatgg cgagtcatta ataatttatc
    15661 caatcttggg ttattctttt agattgaagt gtttaaaact aaaaaagcat ttatgaagtc
    15721 gtaatagtca ggcttcaaaa tgctttttat tttgtttgtt ttttctttgc cacataaata
    15781 cacctagact gacgatgaag agcatatcca acagatttcc tgcgacttgg atcgtcgaag
    15841 ggttggtcag tggcattaaa gcaaggacaa aaaaggaaaa caagctgtaa ataaaacagg
    15901 tgacaggaac taatcgaaag aagagcggac atttcaattg gctaaaaatg agtgaaaaaa
    15961 taaaataagt aaaaatggat aaaatcacga ctaaaccatt catgataaag aagaaaagcg
    16021 tattgtgatt aaacgaataa aaaagaattg ctaaaaaaga aaacgtttta aatgcaatca
    16081 atacgcttga agcttcgttt aacgtaccag gatgtcgatg aaaaaaatag gcaccgaaat
    16141 aaacagaaaa cagtaagccg caaagagaag acaaaatcaa aaaaaacgga ttttcttcgg
    16201 cttctgtgat atcagaatga tccaaaagca accagcctaa tgcactagaa ggcgcatgtg
    16261 aggtcttaag gtatgaataa catagaaaaa ataagatcgt caaagttgca aaaaaagagc
    16321 ctaaatgtaa aaaattagtg gttctacgtt gaatttccat aatatctcca aat
//
LOCUS       DACDKM010000061        16192 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20834_2016
            SAMN12640370-rid9891663.denovo.061, whole genome shotgun sequence.
ACCESSION   DACDKM010000061 DACDKM010000000
VERSION     DACDKM010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640370
            Sequence Read Archive: SRR10040891
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16192)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16192)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742835.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742835.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:04:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,012
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 29 of 75
            Pseudo Genes (incomplete)         :: 33 of 75
            Pseudo Genes (internal stop)      :: 19 of 75
            Pseudo Genes (multiple problems)  :: 5 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16192
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640370-rid9891663.denovo.061"
                     /isolate="E20834_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(<1..258)
                     /locus_tag="IV334_001758"
     CDS             complement(<1..258)
                     /locus_tag="IV334_001758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8943824.1"
                     /translation="MERQESIREVQEVPTEQAKETQEGRSFSYGKNFSFLRPSIQISV
                     FVLWRIHNNRYSVGSQLFYQEICHALSKKYSQNAYKEALAFL"
     gene            complement(274..924)
                     /locus_tag="IV334_001759"
     CDS             complement(274..924)
                     /locus_tag="IV334_001759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8943825.1"
                     /translation="MKKRFIYVCVGGLFLFGVGSTFFLIHEKLQLTAKEEKLTVEINK
                     FQEEKKGQEDLVTSLKEQLQQVESEVTTLKKANEELKNNDSTEKGNIQTFQKTVTEAF
                     EVLFNYEPDSFQERKNNCAPYLSDKLMKQYFKEGVSYGDSNGVSSELEKCSVYLKSTQ
                     KATIQGLVVVSYRNKIGDSSWNNAMNIFDVTFDPASKKITFMQNLGSSFNQQLLEE"
     gene            complement(935..2107)
                     /locus_tag="IV334_001760"
     CDS             complement(935..2107)
                     /locus_tag="IV334_001760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HAP8943826.1"
                     /translation="MKKKLLLWCGFGCLPTLLLCMFGFLFMILLISSGSEEEEIDCSP
                     TEIVASTISLTNQGMETNGLQIAKEVQKHIPNTTLEGLAGMLGNFQQESSMSPTAIER
                     PNDPLSGHGIAQWTAGRTTALKNFAKERNKEWSDLGVQVEFLIHELNTTEKASQVALQ
                     AKSVEEATEQWQVKFERAGIPMMGNRITFANQWYAKLGKNDSDPVASITLENGANAGS
                     KDLTDYCLETTGTSSSSDSNVLNVAKSLIGYFHYSQPQRAQFGSVENPDKNGYADCSS
                     FVWLVLTKAGYKTPANVGWFTGSMTSDARGSKQYLQEIPESEAKAGDIIIVNLGSGVG
                     NDGHTAVLAEDWHGLSTKVIEQGGMNTNGVGEGQVNLSFGYLLNGGDICFARPIKK"
     gene            complement(2126..2998)
                     /locus_tag="IV334_001761"
     CDS             complement(2126..2998)
                     /locus_tag="IV334_001761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HAP8943827.1"
                     /translation="MKKMILGSLFSATVLLGMVGTSAYADTTSPGDPSVPPTEIIPPT
                     EETPEVPEEPVLPPTDGGEETPEIPEIPETPETPEIPPTEENPDTGGGTETPEVPENP
                     DTGGGEGTPEVPPVEENPGNGGNTETPEKPSNPGGNEGNTTPPTTPPTNNGGTNTTPP
                     VQDVVVKPDGSIGSQQTGGQNVQIETNNASELTHIPTPSTPIETASGEKIVSVVDGVA
                     YKESGNGLTPISDKVEQLPSGNIAVKGSDGKMKVLPKTGEEVSLVATALGGLLASGSG
                     FAFWRKRKATQKMN"
     gene            complement(3020..3940)
                     /locus_tag="IV334_001762"
     CDS             complement(3020..3940)
                     /locus_tag="IV334_001762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8943828.1"
                     /translation="MMMIRFTEVDNMSLLTDKKIALDKAFIQLKRENEELLARAEIGW
                     CTLEIYTENHEIPVFEGSYDFPVEEFHLISLIEQSLTENGQLSEEAQTFIQTLNSELP
                     KFYQVKKDQKNERRPTKIKKEKKTPLSVETAFTQSNGSLQKKKMSFQWLLFGFIVVVS
                     CGLFIMQLSTQREIEQLTKKEHQLQERIAFAEELATKQPQIDTFSRYFITNYYSGEMA
                     EERYRKKLQSFLTEKVAKQISPQKEKVKSILSWEMKKEGKHWQIAYVITLETEKKTQE
                     TKKVSFQLEEQEKNYLVSTLPELDDFEINQ"
     gene            complement(3940..4209)
                     /locus_tag="IV334_001763"
     CDS             complement(3940..4209)
                     /locus_tag="IV334_001763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8943829.1"
                     /translation="MIIVTGIVLLGIGVLFGYHFAYFPKIGEWQQRQAIQKYFQVKGN
                     EYTYYAEDEDSYIVSLNNQEYRVKFSLNNPIQVVYAVEVVEEVFD"
     gene            complement(4206..4586)
                     /locus_tag="IV334_001764"
     CDS             complement(4206..4586)
                     /locus_tag="IV334_001764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HAP8943830.1"
                     /translation="MITNYKKFWQNSFNYKGKATRKEYWLVALMNALIGFGLGLAIGL
                     AGALNASETIIGCLIGVLIVYCFVVLVPSLSLSVRRLRDAGFHWAFIFFYFVPTIGSL
                     VLLVLYCLPTKESIEDKQVQEETR"
     gene            complement(5053..6516)
                     /locus_tag="IV334_001765"
     CDS             complement(5053..6516)
                     /locus_tag="IV334_001765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HAP8943831.1"
                     /translation="MKKTSKCLSALLLVGQVFGYTPAVLANEQGNNQLTNSQSATITE
                     ETTTNSTGEENQPETPTEDTIDTPDNPEMNAPPTGSEEQPETPNQPDKPEDNQQPEEE
                     PSEEPNEQPSDESSQTPQKPSTETEAEPPTKTERPQKAPEQSVTVPMNDVPQAQSIGS
                     EETKNYHQAPSAPIQELVPSSTQDNEGTIHFEKDASVETFIRKIGESARKIGQENDLY
                     ASVMIAQAILESASGQSQLAQAPNYNLFGIKGTHNGKGVSFATQEDLGNGTLYTTQAT
                     FRQYESYEESLKDYAKLLKEGLVGNTSFYDGVWKSKAKTYQEATKFLTGRYATDTSYD
                     KKLNGLIETYDLIKYDKEVAGPQLSKEGYMVPLKNYSISSSFGWRGEEFHRGLDMAAN
                     QGEPIYASKAGTVIEASYHTLSGNYVTIEHEDGSVALYAHQQEYCVNVGQTVEQGQII
                     GYVGSTGNSTGPHLHLELALDGSLTKDKLINPETVLF"
     gene            complement(6580..9597)
                     /locus_tag="IV334_001766"
     CDS             complement(6580..9597)
                     /locus_tag="IV334_001766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HAP8943832.1"
                     /translation="MFKRMATWLAIFVTFLNPMLGSVTAFAETINESNSTEEVMKTEE
                     SKSSEEAISTETTSETTEETTTQDTTEDVSPPNVMTQEVPEGYEKYFITIEDIQKATS
                     FSEQERLAQESEEWQAILEKNAQTRATNLTLTENGRKDVNMTRITPEGTMYTHDYMVR
                     WQIDGEDVFCIQEGAITSAGIQYDVTPLENLITTSAIRKKISLIGYFGYYSSGSMDNY
                     VLAQTYIWEVLGGSFVNYGTIGQSNYEAFKQTVDRKINNLLEDPSFDSQTVTVKVGEE
                     VEVKDTKGVFSQWDGSVVENTANVQVRKSGNSLFIKANQNSNDGRISVSSLDPNSRGV
                     GSSFAYVDPVAQNLGKLYVPDPNRAGLTIKVLKNGDFKLTKLDAVTGKKVPNVTYEIK
                     RHDTGETKQYTTDAKGEIALKEIPAFYEKDGKYVQNKLDVKEVKAPAGYILDKQTHTI
                     TIEPNKEVTLTAKNDLQQFIPEGKKVKEVYNAEESWNQGKPVYDTVPAENIEFDIIAK
                     TDITAPDKETVLVKKGEVLDSVKTDKDGHWKANKKFYNGDQNKYQLIEKNVPDNYRQP
                     SEEQTTFSVAYGDNTTPVLTFELDVIDNLLKTGEVDFNKVDSINHLQLPGAKFLIEGM
                     TAHNKNVSFVYETKEEPEKLKLPDGTYKFTEIVFPEHYGQASGQTETILVTVKDGEVQ
                     PITVKNEEIVVPEEDQPEISTLFVTTTGGKDFNPKKDQELVDKVSQVFPKSAVGTEKY
                     WVTQFHKITADGKVTVLDTVKETRKVTDEKETFDVTFNYKADMVSDGDTIVATHIVYN
                     DKEEEEEFARHFDLTNKDQTVTAKEPKIETRFITVNGDQTFDPDKDQELIDHVSQEFP
                     ESTVGETKYWVTQFHKIDKNGKDTVVGTVETARKVSKASEEFKVSFDYKAGTLKDGEK
                     LVATHIVYKDKDHKEEEARHFDLKNEKQTLTAKGIKKETPTTPVKQTPTSTPKQSLPK
                     TGETVNDVLIALGLFLFVGVGAVLVRRKMKQSN"
     gene            complement(9748..10587)
                     /locus_tag="IV334_001767"
     CDS             complement(9748..10587)
                     /locus_tag="IV334_001767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HAP8943833.1"
                     /translation="MKMLKVLALGIVVGGVSLAVGTNAEAAENKVWTPRSAEQIKAEI
                     KGNEYTIVWGDTLSAISQATNITVQKLADMNKITNVDLIYAGNKLVFNGNVASVQNSA
                     GETVAQTVIQEKDKVDPNQPVGQGTTSNTQTTPNTTTPTTPAGDQTGTTGGTTTPSTP
                     ENNGGTTQTPTQPGNGGNTGGETTPSNPSTGGGTTTPEEPSNPGGGDQGGNGGTTTPE
                     EPTIVVGEIGNSGKVFNTVDEAYDWANAEVSDPNSQWYNKGFMVDVIIYSDGSQKHTV
                     DFY"
     gene            10732..10977
                     /locus_tag="IV334_001768"
     CDS             10732..10977
                     /locus_tag="IV334_001768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014862476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8943834.1"
                     /translation="MQLNNLNEIHDWAQNNLLNRKQAAKITGQNYDAFSSSLRRNNIK
                     PFLEIGDEPGPSIIRLYLREDIEKYAKQLEEKRQKRQ"
     gene            11040..11462
                     /locus_tag="IV334_001769"
     CDS             11040..11462
                     /locus_tag="IV334_001769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014862359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8943835.1"
                     /translation="MNKKYIVSVGSIVLIVLAIIAFSVSNKTETQPQNTVQSTTTSQS
                     SDHSVYPSLNSLKETHGQDLIDLGSSQITTVLFEKKNIAFITPKEGTDLEFSIKVTNT
                     KENLFSIPDIQYTPNKLKIGDTFGLAKVNEQYFAYSLK"
     gene            complement(11556..11873)
                     /locus_tag="IV334_001770"
     CDS             complement(11556..11873)
                     /locus_tag="IV334_001770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8943836.1"
                     /translation="MPVKGTSNFDFEIFYVDDEICGVFNKQKYTKEQAIEAWKNELFD
                     PEEEISFVVDESFVGWYHGNAEEEEVHYAGWWLEEGEPREQSVPVWVISENPLEEIEK
                     LEK"
     gene            complement(11900..12106)
                     /locus_tag="IV334_001771"
     CDS             complement(11900..12106)
                     /locus_tag="IV334_001771"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8943837.1"
                     /translation="MNWLKNEAELYVFHEEMQEKHLVPNVRNYLFWLEEKNDAQVFGK
                     SAQSPKIKLQLTSKQWNEHEWYIY"
     gene            complement(12087..12269)
                     /locus_tag="IV334_001772"
     CDS             complement(12087..12269)
                     /locus_tag="IV334_001772"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8943838.1"
                     /translation="MTKCERVSVTISFIALLLSVISLLNTAIFIGQVEKQKEISLSEW
                     RYEEVNEGMNNELVEK"
     gene            complement(12283..12633)
                     /locus_tag="IV334_001773"
     CDS             complement(12283..12633)
                     /locus_tag="IV334_001773"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8943839.1"
                     /translation="MKKSYKVFENNEIKEQEAIILSAQTPEEALEQALPFLVESQNIV
                     VEELPEFDSKIVFSFNCKKESEISVSPEFYCRSDISVEGLVMCASAFIEIFANHLQIS
                     VKELLDLLQEITNE"
     gene            complement(12659..12961)
                     /locus_tag="IV334_001774"
     CDS             complement(12659..12961)
                     /locus_tag="IV334_001774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8943840.1"
                     /translation="MLSKQEKKYIRQKMYAMIGNRAITELSSEELLQVQQLAQIIGSN
                     SIFDSNPLPEMTLETLTAKRYKELRAIGYRMIDIRRALRISDTKFREWRTSNGLAN"
     gene            complement(12964..13197)
                     /locus_tag="IV334_001775"
     CDS             complement(12964..13197)
                     /locus_tag="IV334_001775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8943841.1"
                     /translation="MTISVGNKVKYIGTAIPRYTNKLVVVKNILVNGLILEYPKEDRR
                     KVVVEGNGTWNMNSFICGFTEVEVTNDNENRSG"
     gene            complement(14183..14505)
                     /locus_tag="IV334_001776"
                     /pseudo
     CDS             complement(14183..14505)
                     /locus_tag="IV334_001776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347542.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(14628..15743)
                     /locus_tag="IV334_001777"
     CDS             complement(14628..15743)
                     /locus_tag="IV334_001777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8943842.1"
                     /translation="MATKRFTATRKYNELYYQFPKVFITSEKYRSLSDKAKIAYMVFR
                     SRLDLAIQRNQVDSQGFVYFEFTEQELANALNCCRRSVNTIKKELKEYELLEIEDMGF
                     DQQTGKRNKARLYLGELDTTENDVYSLGPVDSTSVDSAQGKEEEESVDNLAIFDDFDE
                     KNPCENSAQLFNNSSNNLDTENLDTKIDTTSPVEMADDWQKEFIAHYSESTFVPKDVL
                     TWIVRSSAEWREAKEQLNMIYDSKRIIEKRQRKYNQTHGLSDGSEYRILGETFAEDLS
                     KTYQRCIQNEKIARHEGKAIQNRKGFYFRALLNFWECCVRELNLDPMATKLSDQAEYS
                     LLQAKRSCSYEDLETQIYGLSQLPKISMHNWLEELDT"
     gene            complement(15749..16147)
                     /locus_tag="IV334_001778"
     CDS             complement(15749..16147)
                     /locus_tag="IV334_001778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8943843.1"
                     /translation="MVKNKFRATDVLTQATFHMPKLFFIKSSKYYAMTAQAKYAYSLL
                     NEYLEESLIEKRIDEKKHVYISYTNQELAILLNVSEEEIEEIKNELEDYDLLTIEFGR
                     IYLKELIATMEDVRVYQHQNSFLIYGQDEV"
BASE COUNT         4274 a         3398 c         2542 g         5978 t
ORIGIN      
        1 caaaaaggca agtgcttctt tgtaggcatt ttgactgtat ttttttgata aagcatgaca
       61 aatttcttga taaaatagtt gactgcctac cgaataccga ttattgtgta tccgccacaa
      121 aacgaacaca ctgatttgaa tcgacgggcg taaaaaagaa aaattctttc cataagaaaa
      181 agaacgtcct tcttgggttt cttttgcttg ttctgttggt acttcttgta cctctcttat
      241 tgattcttgt ctctccataa ccttctcctt ttcttattct tctaaaagct gttgattaaa
      301 gctacttccc aaattttgca taaacgtgat tttctttgac gctggatcaa aggttacgtc
      361 aaaaatattc atggcattat tccaggaact atcaccaatt ttattgcgat acgacaccac
      421 aacgagccct tggatagtcg ctttttgtgt gctttttaaa taaacagaac atttctctaa
      481 ttcagaagac accccgtttg aatcaccata ggaaacgcct tctttgaaat attgcttcat
      541 cagtttgtca gaaagatatg gtgcacaatt gttttttcgt tcttgaaaag aatcaggctc
      601 ataattaaac agaacctcga acgcttctgt cactgttttt tgaaaggttt gaatgtttcc
      661 tttttctgtt gaatcattgt ttttcaattc ttcatttgct ttttttaacg tcgtgacctc
      721 gctttctact tgttgaagtt gttctttgag tgacgttaca aggtcttcct gtcctttctt
      781 ctcttcttgg aacttgttta tctccaccgt cagtttctct tcctttgctg ttagctgtag
      841 cttttcatga ataagaaaaa atgtactgcc tacaccaaat aagaacaatc cgcctacaca
      901 aacataaata aatcgtttct tcatttgaca cctcttattt ctttatcggt cgagcaaaac
      961 aaatatcacc accattaagc aaatacccaa aggataaatt gacttgccct tcacctacac
     1021 cgttagtatt cattccccct tgttcgatta ctttcgtaga aagtccatgc caatcttccg
     1081 ctaaaacagc tgtgtgtcca tcatttccca caccagaacc taagttcaca atgataatat
     1141 cgccagcttt tgcctcgctc tcaggtattt cttgtaagta ttgcttactt cctcttgcgt
     1201 cgctcgtcat tgagccggta aaccacccaa cgttcgcagg cgttttatac cctgctttcg
     1261 ttaaaacaag ccacacaaag ctcgaacaat cggcatagcc atttttatct ggattttcga
     1321 cactaccaaa ttgtgcccgt tgcggttgtg aataatgaaa ataaccaatc agactttttg
     1381 ctacgtttaa tacattacta tcggaagaac tgcttgttcc tgttgtttct agacaataat
     1441 cggtcaagtc tttacttcct gcgtttgccc cattttctaa tgtaattgaa gcgacgggat
     1501 cactgtcatt ctttcctagt tttgcgtacc attgattcgc aaaagtaata cgattcccca
     1561 tcattggtat ccctgcacgc tcaaatttca cttgccactg ttctgttgct tcctctacgc
     1621 tttttgcttg taatgcaact tgtgaagctt tttctgttgt gtttagttca tgaatcaaaa
     1681 actcaacttg tacacctaaa tctgaccact ctttgtttcg ctctttggca aaatttttca
     1741 aagcagtcgt tcgccctgcc gtccattgtg caatcccatg cccagataat ggatcgtttg
     1801 gtcgttcaat cgctgtggga ctcatactcg actcttgttg aaaattgcct aacatacccg
     1861 ctagaccctc taatgtcgta tttggaatat gtttttgtac ttcctttgca atttgaagcc
     1921 cgtttgtctc catgccttgg ttcgttaatg agatagtact tgcgacaatt tcggttggac
     1981 tacagtcgat ttcttcctct tcagaaccac tactaatcaa aagtatcata aacagaaaac
     2041 caaacataca tagtaatagt gtgggtagac aaccgaaccc acaccataaa agaagttttt
     2101 tcttcatgtt tgatccttca ttcgcttagt tcattttttg tgttgctttt cttttgcgcc
     2161 aaaaagcaaa accgcttcct gaagccaata atccaccaag tgctgtcgca acgagagaca
     2221 cttcttcgcc agtcttaggc aatactttca tttttccatc agaccctttc accgcaatat
     2281 ttccgcttgg aagttgttcg accttgtctg aaatgggcgt caagccattt cctgattctt
     2341 tataagcgac accatcaacg acggagacaa ttttttctcc tgaagctgtt tcaattggtg
     2401 tacttggtgt tggaatatgg gttaattcag acgcattatt ggtttcaatt tgaacatttt
     2461 gtccacctgt ttgttggctg ccaatacttc catctggttt tactacgacg tcctgtactg
     2521 gaggcgtagt gttcgtcccc ccattattgg taggtggtgt cgttggagga gtcgtatttc
     2581 cttcattgcc tcctggattc gacggctttt ctggtgtctc tgtattacca ccatttcctg
     2641 ggttttcctc aacgggtgga acttcaggtg ttccttcccc tccgccagta tctggatttt
     2701 ctggtacttc aggcgtttct gtgcctccac ctgtatctgg attttcttcc gttggtggaa
     2761 tctctggggt ttcaggagtt tctggtattt cgggaatttc tggggtttct tctcctccat
     2821 ctgttggtgg taaaacaggt tcttcaggaa cttcaggtgt ttcttccgtt ggtggaataa
     2881 tttctgtcgg tggtacactt ggatcacctg gtgacgtagt atctgcatac gcactggttc
     2941 ctaccatacc tagcaataca gttgcactaa ataaacttcc taaaatcatt tttttcatgt
     3001 gtttttcctc ttctcttatt tattgattta tttcaaaatc atctagttcg ggaagcgtgg
     3061 aaactagata atttttttcc tgttcttcca attgaaaaga cacttttttt gtttcttgtg
     3121 tctttttctc ggtttccaat gtaatgacat acgcaatttg ccaatgcttg ccttctttct
     3181 tcatttccca ggataaaata cttttgactt tttccttttg aggactgatt tgtttcgcaa
     3241 ctttttccgt tagaaacgat tgaagctttt tcctataccg ttcttctgcc atttcacccg
     3301 aataataatt cgtaataaaa taacgactaa atgtatcaat ttgtggttgt tttgtcgcaa
     3361 gttcttccgc aaatgcaatg cgttcttgta actggtgctc ctttttcgtt agttgttcaa
     3421 tctctctttg cgttgaaagt tgcataataa aaagaccaca agatacaacg acgataaatc
     3481 caaacaaaag ccattgaaaa gacatttttt tcttctgaag agagccgttg ctttgtgtaa
     3541 acgctgtttc tacagaaagt ggtgttttct tttccttttt gatttttgtt ggtcgacgtt
     3601 cgttcttttg gtctttcttt acttgataaa acttcggtaa ttcggaattt aaggtttgaa
     3661 taaaggtttg tgcttcttca cttaattgcc cattttctgt taaagattgt tcaatcaatg
     3721 aaatcaaatg aaactcttct actggaaaat catacgagcc ttcaaatact ggaatttcat
     3781 ggttttctgt gtagatttcc aaggtacacc agccaatttc cgctcgtgcc aatagctctt
     3841 cattttctct tttcaattga ataaatgcct tatctaatgc aattttttta tctgttagta
     3901 aagacatgtt atcaacttct gtaaatcgaa tcatcatcat tagtcaaaca cttcttccac
     3961 cacttctacc gcatacacca cttgaatagg gttatttaaa gaaaatttca cccgatattc
     4021 ttgattgttc aaactgacga tatagctatc ctcatcttca gcataatagg tatattcgtt
     4081 gcctttgact tgaaaatatt tttggatcgc ttgtctttgt tgccattcgc caatctttgg
     4141 aaaataggcg aaatgatagc caaataaaac gcctatccct aataaaacaa ttcctgttac
     4201 tataatcatc tggtttcctc ctgtacctgt ttatcttcta ttgactcttt tgtcggtaag
     4261 caataaagaa ccagtaacac aagagagcca atcgttggga caaaatagaa aaaaataaat
     4321 gcccaatgaa aacctgcgtc tcgtaaacgt cgaacgctga gagaaagaga cgggacaaga
     4381 actacgaaac aatacacaat caatacgcct atcaaacaac caattattgt ttcagaagca
     4441 ttcaacgctc ctgctagtcc aatcgctaag cctaggccaa acccaatgag ggcattcatt
     4501 aacgcaacga gccaatactc ttttcttgtg gctttccctt tgtaattaaa gctattctgc
     4561 caaaattttt tataatttgt gatcatatct ttgtcctttc taatgtttat ttttgattct
     4621 agtttcttct gttttactaa caatgaaact tgccataaaa aaggtggcca atacaccacc
     4681 agctagaaat ccacttacaa ataacaaaat tttttcctcc tttctaaaaa atttcgccat
     4741 aaaaaggcaa agtatgaaca cgataaatac catgtacata ctttgccttt ttatgcaata
     4801 tatgttaact ctccaagcaa ttactgattt ttaaattctt tataaataaa ataaaaatcg
     4861 tttgagtaaa gaaataagtc aatgaagacc caaacgataa aaaatattcc taatactata
     4921 tttaataaac tacgttctag atagatacga gatttcaaat taaaaaacga aaatgagaga
     4981 tacaaatata tcaaatattt aaaaagtaac cacattttct ataccactcc ttatcagaaa
     5041 cagccttatt ctttagaata agactgtttc tgggttgatt aatttgtctt ttgttaaact
     5101 accatctaag gcaagttcta aatgtaaatg tggtcctgta ctatttcctg ttgaacccac
     5161 ataaccaata atttgtcctt gttcaactgt ttgtcctacg ttcacgcaat attcctgttg
     5221 gtgcgcataa agagccacac ttccgtcttc atgctcaatt gtgacataat ttcctgacaa
     5281 agtatgatac gaagcttcta tgaccgttcc tgccttgctc gcatagattg gttctccttg
     5341 attggctgcc atgtctaatc ctctatgaaa ttcttctcct cgccaaccga aggaactaga
     5401 aattgaataa ttttttagag gtaccatata gccttctttg cttagttgtg gaccagctac
     5461 ttctttatca tatttgatta agtcataggt ttcgattaac ccatttaatt ttttatcgta
     5521 gctagtgtct gttgcgtatc ttcctgttaa aaatttagtc gcttcctgat aggtttttgc
     5581 cttacttttc caaacaccat cataaaaaga ggtatttcca actaagcctt cttttaacag
     5641 tttggcatag tcttttaagc tttcttcata gctctcatat tgccgaaaag ttgcttgggt
     5701 cgtataaagt gtcccattgc ctaaatcttc ttgtgtcgca aaagagacgc cctttccgtt
     5761 atgtgtgcct ttgattccaa atagattgta gtttggtgcc tgagccaatt gactttgtcc
     5821 gcttgcggat tctaaaatgg cttgcgcgat cataacagaa gcgtataaat cattttcttg
     5881 tccaatttta cgagcagatt caccgatttt acggataaac gtttctacag acgcgtcttt
     5941 ttcaaaatga atagtccctt cgttatcttg tgtgctggaa gggactaatt cttgtatcgg
     6001 tgcgcttggt gcttggtggt agttttttgt ctcttctgaa ccgattgatt gtgcctgcgg
     6061 tacgtcattc ataggtacag tcacactctg ttcaggtgcc ttttgtgggc gttccgtttt
     6121 tgttggcggt tccgcttctg tttcggtact tggtttttga ggagtctgtg agctttcatc
     6181 agaaggttgc tcatttggtt cttctgacgg ttcctcctca ggttgttgat tgtcttctgg
     6241 tttgtctggt tgattcggtg tttctggttg ttcttcagaa cctgttggtg gagcgttcat
     6301 ttctggatta tctggtgtat ctattgtatc ttcagtcggt gtttctggtt gattttcttc
     6361 tccagtggaa tttgttgtcg tttcttcagt gatcgttgca gattgcgaat ttgtcagttg
     6421 attgttgcct tgttcattag ccaaaaccgc tggcgtatac ccaaatactt gacctactaa
     6481 taacaaagca gataaacatt tactcgtttt tttcacaaga ttgcctccat tcttcaatca
     6541 atcatctaga gacagctgat tgctgtctct agtcatactt taattgcttt gtttcatttt
     6601 acgacgaacc agaacagccc ccacaccaac aaaaagaaac agtcccaatg caattaacac
     6661 atcgttcact gtttctcctg tttttggtaa gctttgtttt ggtgtgctcg tcggcgtttg
     6721 tttgacaggg gttgtcggcg tttctttttt aatgcctttt gctgtcaatg tttgtttttc
     6781 attcttcaaa tcaaagtgac gtgcttcttc ctctttatgg tctttatcct tatagacaat
     6841 atgagttgcg accaatttct ccccgtcttt taacgtgcct gctttgtagt caaaggatac
     6901 tttaaactct tcagacgctt tagaaacttt acgtgcagtt tctaccgtac caacgactgt
     6961 atcttttcca tttttatcaa tcttatggaa ttgagtcacc cagtatttgg tttcccctac
     7021 cgtagattct ggaaattctt gcgatacatg atcgatcaac tcttggtctt tgtctggatc
     7081 aaatgtttgg tcgccattca ctgtaataaa gcgtgtttcg atttttggtt ctttcgcagt
     7141 cactgtttga tctttattgg ttaaatcaaa atgacgagcg aattcttctt cctcttcttt
     7201 gtcgttatag acaatgtgcg ttgcgacaat cgtgtcaccg tctgaaacca tgtctgcttt
     7261 gtagttgaac gtcacatcaa atgtttcttt ttcatcggtc acttttcgtg tttcttttac
     7321 cgtatctaat acagtgactt ttccatcggc agtaatttta tggaattgcg tcacccaata
     7381 tttctctgtc ccaaccgcag atttagggaa tacttgggac actttatcca ctaattcttg
     7441 gtcttttttc gggttgaaat cttttccacc tgtcgttgtc acaaacaaag tagaaatttc
     7501 tggttggtct tcttctggaa ccacgatttc ttcgtttttc acagtaattg gttgtacttc
     7561 tccgtctttg accgttacta aaatcgtttc tgtttgtcct gacgcttgcc cataatgctc
     7621 agggaacaca atttccgtaa atttatacgt accgtctggt aattttagtt tttctggttc
     7681 ttcttttgtt tcatacacaa aggacacatt tttattatgt gcagtcatac cttcaatcag
     7741 gaatttcgct cctggtaatt gtagatgatt gattgaatcg actttattga agtctacttc
     7801 acccgttttt aacaagttgt caatcacatc taattcaaac gtaagcacag gcgtcgtgtt
     7861 atcgccatag gccacagaga acgtggtttg ttcttcagaa ggttgacggt agttgtctgg
     7921 cacatttttt tcaatcaatt ggtatttgtt ttgatcgcca ttgtagaatt ttttgtttgc
     7981 cttccagtgt ccgtctttgt cagtttttac tgaatccaag acttctcctt ttttcacaag
     8041 gactgtttct ttatctggcg cagtaatatc tgttttggcg ataatatcaa attcgatatt
     8101 ttctgcagga acggtatcat aaacaggttt gccttgattc caggattctt ccgcattata
     8161 gacttctttg actttctttc cttctggaat gaactgttgt aagtcatttt ttgcagttaa
     8221 cgtcacttct ttgtttggtt caatcgtaat cgtatgagtt tgtttgtcta gaatataccc
     8281 agctggtgct ttgacttctt tgacatccaa tttattttga acatattttc cgtctttttc
     8341 atagaatgca gggatttctt ttaaggcgat ttcccctttg gcatccgttg tatattgttt
     8401 tgtttctccc gtatcatgac gtttgatttc atacgtcaca tttggaactt ttttccctgt
     8461 gacagcgtct agtttggtta gtttgaagtc gccatttttt agaactttaa tagttagtcc
     8521 agctctatta ggatctggca cataaagctt tcctagattt tgagcaacag gatctacata
     8581 ggcaaaactt gaacctaccc ctctagaatt tggatccagt gaagaaacag aaattcgtcc
     8641 gtcatttgag ttttgatttg cttttataaa caaactattt cctgattttc ttacttgaac
     8701 gttagctgtg ttttcaacaa ccgacccatc ccattgagaa aacacacctt ttgtatcctt
     8761 aacttctact tcttccccta ctttaaccgt cactgtttgg ctatcaaaag aaggatcttc
     8821 aagtaaattg tttatttttc tatctacagt ctgtttgaaa gcttcataat tagattgtcc
     8881 aatagttcca taattaacaa atgaaccacc taaaacttcc caaatatatg tttgtgctaa
     8941 tacatagtta tccattgaac cactagaata gtagccaaaa taaccaatta aagaaatctt
     9001 ttttctaata gcacttgtcg taattaaatt ttcgagtggt gtcacatcat attgaatacc
     9061 cgcactcgtg attgcgcctt cttgaatgca aaaaacatct tctccgtcaa tttgccagcg
     9121 aaccatgtaa tcatgagtat acattgttcc ttcaggagta attcgagtca tattcacatc
     9181 tttacgacca ttttcagtca atgttagatt ggtagcacgt gtctgtgcat tcttttccaa
     9241 gattgcttgc cattcttcag attcttgcgc caatcgttct tgttcagaaa aagaagtcgc
     9301 tttttgaata tcttcaatgg ttataaagta tttctcataa ccttcaggga cctcttgagt
     9361 cattacattg ggcggagaaa catcttctgt cgtgtcctgc gtagttgtct cttctgttgt
     9421 ttcacttgtg gtttcagtac tgatcgcttc ttcactactt tttgattctt cagtcttcat
     9481 tacttcttca gtagaatttg attcatttat cgtttctgca aatgccgtta cagatcctaa
     9541 catgggattg agaaatgtaa cgaaaattgc caaccaagtt gccattcttt taaacatatt
     9601 tttttcgttc ctttcatttt atttttcagt cttctaaagt aatttcttat ggacttcgac
     9661 accgaaacat ttttatcctc ctcaaaacaa aaaaagacta gaacttataa ttctagtctt
     9721 catcaaccac ttacttttct tttattttta gtagaaatct acagtgtgtt tttgtgaccc
     9781 gtcactgtaa ataataacat ccaccataaa acctttgttg taccactgcg aatttggatc
     9841 gctaacctct gcattagccc aatcataggc ttcatctaca gtgttaaata cctttccact
     9901 attaccaatc tctccaacta caattgttgg ctcttcaggt gtcgttgtgc caccatttcc
     9961 gccttgatcg cctccaccag gattgcttgg ttcttcaggc gttgttgttc ctccacctgt
    10021 gcttgggtta gaaggagttg tttctcctcc agtatttcca ccattacctg gttgtgtagg
    10081 tgtttgagta gtgccaccat tgttttctgg tgtacttggc gttgttgtac caccagttgt
    10141 tcctgtttga tctccagctg gtgttgttgg tgtcgttgta tttggtgtcg tttgtgtatt
    10201 tgaagtcgtt ccttgtccta ctggttgatt cggatcaact ttgtctttct cttgaatcac
    10261 tgtttgagcc acagtttcac ctgcgctgtt ttgtacactt gctacattgc cattaaatac
    10321 taatttattt cctgcataga ttaaatccac atttgtaatc ttattcatgt ccgctaattt
    10381 ttggacagta atatttgttg cttgactgat tgcgcttagg gtatctcccc agacaattgt
    10441 gtattcattc cctttaattt ctgctttgat ttgttccgcc gaacgaggtg tccaaacttt
    10501 gttttctgct gcttcagcat tcgttcccac agcaagacta actcctccga ctacgattcc
    10561 tagtgccaat actttcaaca ttttcattgt ttttcgcctc cgatttgaat aaatttattt
    10621 acaaaaacat agtaccaaac ttatgtctat cagtcgatat attttttgta gattttttta
    10681 ttttttgata tactaacttt ggtactaaaa taactaaaag gaatgtcaca catgcaactt
    10741 aataatttaa atgaaataca tgattgggct caaaataatt tacttaatcg taaacaagct
    10801 gccaaaatca ctggacaaaa ctatgatgct ttttctagct ctttacgtag aaataatatc
    10861 aaaccatttc tagaaatagg cgacgaacct ggtccttcca tcattcgctt atatctaaga
    10921 gaagatattg aaaaatacgc aaaacagctc gaagaaaaaa gacagaaacg tcaatgaggc
    10981 gttttctgtc ttggatttac aagtaaaaca ataataaaca cttttacaac ggaggttcta
    11041 tgaataagaa atacattgtt tcagttggtt caatcgtact gattgtactc gctattattg
    11101 ctttttctgt atccaacaaa acagaaacac agccacaaaa tacggttcaa tcgactacta
    11161 caagtcaatc tagcgatcat tccgtctatc cttcactaaa tagtttaaaa gaaacgcatg
    11221 gacaagattt aattgatctt ggttcttctc aaattactac tgtcttattt gaaaagaaaa
    11281 atattgcgtt tattactcct aaagaaggaa ctgaccttga attttctatc aaagtaacaa
    11341 ataccaaaga aaatttattc tccattcctg acattcaata tacccctaac aagttaaaga
    11401 ttggggatac gtttggtttg gctaaagtaa atgaacaata ctttgcttac tcactgaaat
    11461 aagtttctaa tagcaagagg aaaaaccctc ttgttatttt tatcacataa tcattttata
    11521 aaaacgcttc gaaaatatca acatttttga agaaactatt tttctaattt ctcaatttct
    11581 tccaagggat tttcgctaat gacccataca ggcacagatt gctctcgagg ctccccttct
    11641 tctaaccacc aacctgcata atgaacttct tcctcctcag cattaccatg ataccaacca
    11701 acaaaggatt catctacaac gaatgaaatt tcttcttctg gatcaaataa ttcatttttc
    11761 catgcttcaa ttgcttgttc tttggtgtac ttttgtttgt taaatacacc acaaatttcg
    11821 tcatctacat aaaaaatttc aaaatcaaaa ttactcgttc cttttactgg catcttaact
    11881 tttcctcctt tgactcactt caatagatat accactcatg ttcattccat tgtttactag
    11941 ttaattggag ttttatctta gggctttgtg cactctttcc aaaaacttga gcgtcgtttt
    12001 tttcttctaa ccaaaaaagg taatttctta cattcggtac taaatgtttt tcctgcattt
    12061 cttcatgaaa aacatataat tctgcttcat ttttcaacca attcattatt cattccctca
    12121 ttcacttctt cataccgcca ttcagatagt gagatttctt tttgtttctc cacttgtcct
    12181 ataaaaatcg ctgtattcaa caagctgatc acacttaata ataaagcaat gaaactaatg
    12241 gttacactta ctctttcaca ttttgtcatt ttgtttcctt ctttactcgt ttgttatttc
    12301 ttgtaatagg tctaataact ccttcaccga aatttgcaaa tggttcgcaa aaatttcaat
    12361 aaaagcactc gcacacatta ccagaccttc aactgatata tcactgcgac aataaaattc
    12421 tggagaaaca ctgatttctg attctttctt acaattgaac gaaaaaacta tttttgaatc
    12481 aaattccggt aattcttcca caactatgtt ttgggattct accaagaaag ggagtgcctg
    12541 ttctaatgct tcttctggtg tttgagcaga tagaataatt gcttcttgtt cttttatttc
    12601 attgttctca aatactttat aacttttttt cattgttagt tcctctttca ttcctctctc
    12661 aattagctaa gccgttactg gtacgccatt ctctaaattt agtatcgcta atccttaatg
    12721 ctcgtcgaat atcaatcatt cgataaccta ttgcacgaag ttctttatat cttttagcag
    12781 ttagcgtctc taacgtcatt tcaggcaatg ggttcgaatc aaaaatagaa ttcgaaccga
    12841 ttatttgggc caattgttgt acttgtaaca gctcttctga acttaattca gtgattgctc
    12901 gattgcctat cattgcatac attttctgtc gaatatactt cttttcttgt ttagacaaca
    12961 tttctatcca ctcctatttt cattatcatt tgttacctcc acttcagtaa atccacaaat
    13021 aaatgaattc atattccaag taccattccc ttcaactaca acttttctcc tgtcctcttt
    13081 cggatattca agaatcagac cgtttaccaa tatgtttttc actactacta atttgtttgt
    13141 ataccgtgga attgctgttc cgatatactt cactttattt cctacgctaa ttgtcatttc
    13201 ctcactccta ttctttttta tccaaaactt tttcctgtcc tattcctgcc aacgaatata
    13261 cttcactgca tgcttaaaca aaacaaaatg ttgccctgtt ttatttccgt cttttacttg
    13321 ttccacatac agttcatatt tggtcacacc ttttaaaacg ccagatactt tttttcatcc
    13381 attgtcacaa ttagtttttt catcaatcga ttgatactgc actgctcatt tttcttgttc
    13441 atatagtatt cctttctttt tcaaaactag cctcttttgt ttactagttt tcatcaatca
    13501 tcaacgattt aacagatttt ttctttcgca gggtttctag cgtcagcaac cccaaactct
    13561 aaaaaagtgc ctttgctccc ttgaaaaacg agcggacgat tgtactcgtc atcatatgtt
    13621 tttcatacta cctgatccac gcaaataacc tttttttgtt ctgtgccaca cccatccata
    13681 cgtatcgctt gagattcacc atactttttt aggtggttta tccttcctaa gtccctgtta
    13741 gacacgctga ttaacttaga aagtcctgtt tgtatccaat tttcaaagat ctcccaaaag
    13801 aaataagtag gtttacgtgt atcgcttctg ttcaaagtac ctgtttgtac ttgagtattt
    13861 ctactcccct cgttcaaggg attccttcca ccagctagct tttcctttca tcatcaaatg
    13921 ttcctgaaaa gcatttacgt ttcgtgtccg tgtgcttact tattcttttt tcgatagcga
    13981 ttatccgctc gctactcgct attttatact ttgtttgtta gctcaaataa aaactaacaa
    14041 acaaaaaagg ctagtttctt tccgttttat ttttttaact gtcgataaat agttgttagt
    14101 ttaattccaa gttctaaaac caatacgccc aacaaaacca caggaaaaac aattggggca
    14161 aacaagtaaa atcggatagt tttcatgtct tccctctttc tttttggtgt cgctgtggtc
    14221 gttcttgccg ttcaatactt cgatacattt tttattcatt atttgctcgt tttgttttcc
    14281 tctggactgc ttttcttgct ttttgaatac tttatacgct tttcgtgcct gaattaatag
    14341 gtcttgtcgg cgtttcattc gtgagaaaac aatatttgaa accatcaaat cctggttcga
    14401 acaaccacga ttcgcaataa ttttcccatc aaaaaaaata gttttcactt gagaaaaaaa
    14461 cagttggaaa tcttgttttg ttgctaattt ttttgtcaaa atcactgtgc ttttccctgt
    14521 tgaatctaca atgattcttc gaacaatccg attcgataac ttccaacgtt gccgattaaa
    14581 caatgttact tcacaacaaa tatgacgtaa gtgattaggg gtcattttca tgtgtccaat
    14641 tcctccaacc aattatgcat cgaaattttg ggcaattgtg acagcccata aatctgcgtt
    14701 tccaggtctt cataactaca agaacgcttg gcttgaagta atgaatattc tgcttgatct
    14761 gatagttttg ttgccattgg atctagattc aattctctta cacaacattc ccaaaaattc
    14821 aatagcgctc taaaataaaa tccttttcga ttctgaattg cttttccttc atgacgagca
    14881 attttttcat tttgaataca ccgttgatac gttttgctta aatcttctgc aaatgtttcc
    14941 cctaaaattc gatactcgct tccatcagat aaaccatgtg tttgattata ctttcgttgc
    15001 cgcttttcta taattcgttt actgtcgtat atcatgttta gctgctcttt cgcttcacgc
    15061 cattcagctg aactgcgaac aatccatgtc aaaacatctt tcggaacaaa cgtactttcg
    15121 ctgtaatgag caataaactc tttttgccaa tcatcagcca tttcaacagg actagtcgta
    15181 tcgatttttg tatctagatt ttctgtatct agattgttag aagaattatt aaataattgt
    15241 gcagaatttt cacatggatt tttttcgtca aaatcatcga aaatcgctaa attatcaaca
    15301 gattcctcct cttcttttcc ttgtgcagaa tctacactgg ttgagtcaac aggacctaat
    15361 gaatacacat cattttctgt cgtatctaat tctcctagat acaaccgagc tttgtttctt
    15421 tttcctgttt gttgatcaaa tcccatatcc tctatttcta gtagctcata ttctttcaat
    15481 tcttttttga ttgtattaac agaacgtctg caacaattca atgcattggc taattcttgt
    15541 tccgtaaatt caaaataaac aaaaccttga ctatctactt gatttcgttg aatggctaaa
    15601 tctaagcgtg accgaaaaac catatacgcg atttttgctt tatcagacaa tgagcgatat
    15661 ttttcacttg taataaatac ctttggaaat tgatagtata attcattgta tttacgagtt
    15721 gcggtaaatc tttttgttgc catcgttatt acacctcatc ttgtccatat attaagaaag
    15781 aattttggtg ttggtagaca cgaacatctt ccattgtagc aatcaattct tttagataaa
    15841 ttcgaccgaa ttcaattgtt agtaagtcgt aatcttctaa ttcatttttg atttcttcaa
    15901 tttcttcttc agacacattg agtaaaatgg ctaattcttg attggtataa gaaatataga
    15961 catgtttctt ttcatcaatc cgtttttcaa ttaaggattc ttctaagtac tcattcagta
    16021 agctataggc gtattttgct tgtgcagtca tcgcataata ttttgacgat tttataaaga
    16081 atagttttgg catatgaaaa gttgcttgtg tcagcacatc tgttgcgcga aatttgtttt
    16141 ttaccatatt ttttcctcct agaaaaacac gcaaaaaaag ctctgtataa at
//
LOCUS       DACDKM010000062        16089 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20834_2016
            SAMN12640370-rid9891663.denovo.062, whole genome shotgun sequence.
ACCESSION   DACDKM010000062 DACDKM010000000
VERSION     DACDKM010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640370
            Sequence Read Archive: SRR10040891
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16089)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16089)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742835.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742835.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:04:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,012
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 29 of 75
            Pseudo Genes (incomplete)         :: 33 of 75
            Pseudo Genes (internal stop)      :: 19 of 75
            Pseudo Genes (multiple problems)  :: 5 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16089
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640370-rid9891663.denovo.062"
                     /isolate="E20834_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            226..894
                     /locus_tag="IV334_001779"
     CDS             226..894
                     /locus_tag="IV334_001779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF421 domain-containing protein"
                     /protein_id="HAP8943844.1"
                     /translation="MIFSITMKLVFGLIGLLLVVRLLGKKSMSEITPFDLVYTLVLGG
                     ILEESTYDDNVHVGHVLFAIALWAVMIYGIERIVQKNEKVNRWVKGEPSVLIKDGVIN
                     MTELTNNHIEMEQLRAILRQQECFSLENAKHVILENAGQMSVLKKSDEDKALSILLVD
                     EGQIQHKVLQSNQLTEAWLMENLKKEGYADVKQLIYVEWSEEKGLYILNKEDVMHESY
                     RIDG"
     gene            complement(1094..1480)
                     /locus_tag="IV334_001780"
     CDS             complement(1094..1480)
                     /locus_tag="IV334_001780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8943845.1"
                     /translation="MRRTAKKFIDYNEYRDRPFGLKWGTAFAMEELVNGIKKNEEEAR
                     KQPLPKQQMTLQEIDSILTEAFLSRKAVIIQLNLKDEFGRLLDEVEGHFIGEAYEDYF
                     MIDHQPILWEDVRYIELKETKKWFVT"
     gene            complement(1452..2834)
                     /locus_tag="IV334_001781"
     CDS             complement(1452..2834)
                     /locus_tag="IV334_001781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HAP8943846.1"
                     /translation="MWANQNPVFVYEKEPSRDILCIDCKSFYASVECVEKDLDPLTTK
                     LVVMSYPSDSTETRGSGLILASSPAAKKAYGITNISRARDLPFPYPSDLYIVAPRMAY
                     YMEKNREINSIYKKYADEANHHVYSVDESFLDVTNSMALFGKTNAYDMAKLIHKDVFE
                     QTGIYTTIGIGDNPLLAKLALDNGSKDGKQMFAEWRYEDVPEKLWKISDITDFWGIGK
                     RTAKRLKQMGIHSVYDLAHADYYHLKSKLGVIGTQLYAHAWGIDRSFLGQEYTPKSKS
                     IGNSQILDRDYTRREEIEIVIKEMADQVGTRLRRSAAKTQVVSLWVGYSLGYLDPNGK
                     TGFHQQMKIEATNASRDLADALLTIFHQHYHYQDIRRVGVNCSDLVYATGLQLNLFED
                     PEKQINEAKVDFVVDTIRKKYGFQSIIHAHSLLQGGRAIARSSLVGGHAGGMSGLEGG
                     KDETDSKKIH"
     gene            complement(2990..4177)
                     /locus_tag="IV334_001782"
     CDS             complement(2990..4177)
                     /locus_tag="IV334_001782"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HAP8943847.1"
                     /translation="MEIKRSLNTKFAETFGPKKTTQYFSPGRINLIGEHTDYNGGHVF
                     PASITYGTQGVAAPRDDNKILVYSTNFDDIGVISFTLDELEYDKKANWANYVKGMVLK
                     LKEAGHSIDHGFELLVEGTIPNGAGLSSSASLELLVGVVLEDLFDLKTDRLELVQTGK
                     KVENEFIGVNSGIMDQFAIGFGELDHAILLDTNTLKYEMVPVKLDGYAIVIMNTNKRR
                     ELADSKYNERRSECEEALARLQKTLPISSLGDLDEEEFESTIDQIGDDTLIRRARHAV
                     SENQRTLKAKAELEAGNLEAFGQLLNDSHHSLRYDYEVTGIELDTLVDAAQKQEGVLG
                     ARMTGAGFGGCAIALVKEENIPAFKNNVYDEYMKVVGYAPEFYVAHIGNGTTKIDPEA
                     VHV"
     gene            complement(4184..5215)
                     /locus_tag="IV334_001783"
     CDS             complement(4184..5215)
                     /locus_tag="IV334_001783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose mutarotase"
                     /protein_id="HAP8943848.1"
                     /translation="MKITEKDFGQGYHLITLENKNKLALSISDLGARIVSLKSNDREL
                     VLGFDTAEEYIEKDPYIGASIGRTAGRIENGRFSLNGKTYQLATDPKTGHNLHGGAPG
                     FELKKWSYVILNGENEASVIFTTTSPDGEHGFPGTMDVEIRYTLTKDNIWRVTSRGTS
                     DQDTLFNPTNHVYFNLTGDASQSIDQHELWLNSEAYAPLRTDSIPIGVKENAAGSAFD
                     FQIPKKLASVFASDLDQKNLVDGIDHPFFLKETGLGKEAARLTSPDKRIQVGIATDAS
                     SVVIFTANFGTETPEMRNRKLAHHGGITFETQTAPGAERFSAFGSIHLKAGSVFETVT
                     EFKIKTRKE"
     gene            complement(5231..6685)
                     /locus_tag="IV334_001784"
     CDS             complement(5231..6685)
                     /locus_tag="IV334_001784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP8943849.1"
                     /translation="MKSKMIQRVSYALGAFGHDTFYVTLSTYFMVFVTSQMFAGTDKA
                     TNAKMIATVTSLVVGIRLVEIIFDPLIGGIIDNTRTKYGKFKPWLVIGGVVSSVLLVA
                     IFTNFFGLATHNTTLFIILFAIVFILLDSFYSFKDIAFWSMIPALSTETKEREKFATF
                     ARFGSSLGANGTTLVVVPIVTFFTYLVTHKHIEGASGWFWFGVIAAVISGGTAIITAL
                     GTKEVDTAIRQQNEKAGIKDVFKAVAQNDQLMWLALAYIAYAIANVATTAVLFYFFKY
                     VLGQPTEFWIVGLVAALLGLVAVPLFPMLTRVISRRYVYLGGIAMMISSYFFFTIAGS
                     SLPIVIIGLVLFYFPQQLIFLSALMTITDSVEYGQWKNGIRNEAVTLSIRPLLDKIAG
                     ALSNGIVGFVAIASGMTGNATAADITAHGVTTFKAYAFYAPAALMIFSAIIFAWKITL
                     TEKKHAQIVEELENKLAPADTMEDELHDAVVPSH"
     gene            6860..7765
                     /locus_tag="IV334_001785"
     CDS             6860..7765
                     /locus_tag="IV334_001785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HAP8943850.1"
                     /translation="MEQSIFLKKEHQQVEKELYFDFCGFSKTMPFHSFGPAIRNDYIL
                     HVVMEGKGSYHVKNQQFQLKKGDLFLIRPGDSTFYLSDGEDPWMYCWLSFGGSAAEEI
                     IRQSLFREDQYTMVSSDIQKYIDIILECMKDTEETLENELQLTALTYRFLSVLLKDGG
                     KVSGGVQKVHSSLAVETVKYIEAHYAEKLSVEEIAQHLAVNRSHLSRVFKNHMGTSIK
                     EYLIGVRINRGAFLLSLTDESVESIAYQVGFNSLVVFSRMFKKSTGETATNYRKRMKN
                     ENFKNIALDTLKKQLEKQTAVSWTT"
     gene            7856..8533
                     /locus_tag="IV334_001786"
     CDS             7856..8533
                     /locus_tag="IV334_001786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HAP8943851.1"
                     /translation="MRTLIIISHPTIEESLNQQFFKEASAGLPATWHHLESSYPDGKI
                     DIETELQLLAQHDRIIFQFPFYWYSSPAHLKIWQDNVLERAAHVLKGKELGIVLTTGV
                     AEKEYQAGGKEEYTISEFLRPYQRIANKFHMTYLPPFVLAQFMYLSQEKRWEKLIAYQ
                     QYLSLEGKPSLTQRIDWFIQRVQENQKMQEEESEKQAYIVEALTEAKEQIEDLTFTLQ
                     EMKGTSL"
     gene            8814..9710
                     /locus_tag="IV334_001787"
     CDS             8814..9710
                     /locus_tag="IV334_001787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HAP8943852.1"
                     /translation="MYDENEWGENVRMTRRRQQKQAARKRMIMIGAAVAVFLIIAGGV
                     AGLYFHGKNSSEAKSTTITETTTKKTETKQTSESSTKTKETQTSEEKTANGLPEQLQA
                     IVNDYSKKIEEKTPALIEEYQTEIQGNQEGIAGLSAVANQKARELQAISDEGIRKLRA
                     AYQAAENKDGVDLDTLINQLSANYTNHVAKISDIYLQTSASLQAESTSTQDTTSSDSE
                     TAESVEASEMQARTTQDTTDSSETNTNSATSQAATTVVLEGEGPNQIAERTGVPVETI
                     LSLNGMSMENYFLTPGETLKLN"
     gene            complement(9765..11135)
                     /locus_tag="IV334_001788"
     CDS             complement(9765..11135)
                     /locus_tag="IV334_001788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAP8943853.1"
                     /translation="MEEEKLTRSLSARHIQMIALGGTIGVGLFMGASSTIRWTGPSVM
                     GAYAIAGFFLYLIMRALGEMLYVDPSTGSFANYASEYIHPVAGYLTAWSNIFQYIVVG
                     ISEVIAVGSYMNYWWPDLPAIIPGIIVVLFLMLANLISVKAFGELEFWFSIIKVITII
                     LMIIAGLGVILFGFGNHGQAVGISNLWKNGGFFTGGVKGFFFALSIVVASYQGIELIG
                     MTAGEAENPKKTIIEAVQSTIGRILIFYIGAIFIIVSIYPWNQLSEIGSPFVQTFSKV
                     GITFAAGLINFVVITAALSGCNSGIFSASRMVYTLAINGKMSKKFLKLSRHGAPFYPV
                     VAISGGILIGLILNYLLPMFINQSENIFVFVYSSSILPGMVPWVVILISEIRFKKKHA
                     DKMHDHPFKMPLSPYTNYVTLAFLAIVLIFMFINPETRISLIIGLVFLLYMTIHYFVR
                     EKRTGK"
     gene            11373..12119
                     /locus_tag="IV334_001789"
     CDS             11373..12119
                     /locus_tag="IV334_001789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HAP8943854.1"
                     /translation="MERTAIVIQDLSAAYQGKTVLKTINLTINPQKITGIIGPNGAGK
                     STFMKSLLELIPATTGKVTFSGQPVNSVRKKIAYVEQRSELDLSFPIDVLGVVLLGTY
                     PSLRIGQRPGKKEKERARQALKKVGMEEYAKRQISELSGGQLQRVFIARALAQGAEWI
                     FLDEPFVGIDALSERKIFDILQELKNSGKTILIVHHDLHKVEEYFDEVILLNKQLIAS
                     GPVQEVFTSKNLQLAYGEIIRHLVKGGEKK"
     gene            12116..12955
                     /locus_tag="IV334_001790"
     CDS             12116..12955
                     /locus_tag="IV334_001790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HAP8943855.1"
                     /translation="MIQSFIDGLMNYQFLQYALVTSMLVGLASGVIGSFIILRGMSLM
                     GDAISHAVLPGVAISYMFGFSYIFGATAFGMLTAAVIGFVTQRSRLKNDTAIGIVFSS
                     FFALGIILISYAQSATDLYHILFGNVLAVRESDLLLTALVSGIVLIFVFFFYKELKIT
                     SFDPTMAKAYGLNTSLIHYLLMFFLTLVAVVSLQTVGTILVIAMLITPAATAYLLTNH
                     LLKMIITAAGIGMLSAVVGVFFSYSYNWPSGATIVLACTAFFILAFLFSPTKGILFQK
                     GSR"
     gene            12956..13906
                     /locus_tag="IV334_001791"
     CDS             12956..13906
                     /locus_tag="IV334_001791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HAP8943856.1"
                     /translation="MKTKKSLFLILAVSFLVLAGCGKQASDQADEGSKEKLSVVATNS
                     ILADMAKEVGTDQIDIHSIVPVGTDPHEYEVLPEDIKKASDADVILYNGLNLETGNSW
                     FDNLMETAKKEEGKDYFAVSKNVEPLYLTSGEEHTKADPHAWLDLSNGIKYVEEIARI
                     FSEKDAENATLYKKNAEAYVEKLKELDTQAKESFASIEENKKLLVTSEGAFKYFSRAY
                     DLPAAYIWEINTESQGTPDQMKAIIDQIRAKEVPVLFVETSVDSRSMERVAKETGLKI
                     YDKLFTDSIAKEGEQGDSYYQMMKWNIETIHEGLSQTKES"
     gene            14047..14799
                     /locus_tag="IV334_001792"
     CDS             14047..14799
                     /locus_tag="IV334_001792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8943857.1"
                     /translation="MNFTRKDKYTIKEEVFRLISQQENTDKDQDLQFLEAELAKLFIA
                     NQNETTEPDEELYIGKWLFDEFQPLDENTGIGYFVFLSPVGFTPGVLVKQNDEITVKA
                     WPALFSVIKAEGVIKKIQSAYYKSQFIANSKLMNMFYNRLVEIARPGKIPQTFTYTQQ
                     KNLYEEVLYSRDLSEFSPWEEVRLHELLSTKPVSLNFFNALDNKRKLISTNYWEVFAA
                     RKKWMEEKKIADQQIQFVQLKKEKLYYGLYSR"
     gene            14818..15969
                     /gene="queG"
                     /locus_tag="IV334_001793"
     CDS             14818..15969
                     /gene="queG"
                     /locus_tag="IV334_001793"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HAP8943858.1"
                     /translation="MTLKEEIIQESKRLGIDKIGFTTAEPFDSLKESLEEQKAAGHTS
                     GFEHPNIDERLYPEMTFDQPKSIISIALAYPTRIHEEVPRDEKRGQFARASWGIDYHD
                     ILRDRLNRLIEFIQTRAEKWQKEEEWRLAPQVDTGELIDVAVAQRAGLGFIGRNGLLI
                     TEEFGSFVYLGEIVTNIVFEPDKPGEFGCGDCMRCVTACPTKALLGDGRMNAQRCLSY
                     QTQTKGMMPEEYRKKMRNVIYGCDICQLVCPYNQGKDFHFHEEMEPKVDEVYPKLKPM
                     LSMSNKDFKQQFGHLAGSWRGKKPLQRNALIALANLGDRTALPEISECLTDVRPVIRG
                     TAAWAIGRLGTKEPEKWLDVLDEILAKETEEVVINELEHAQKLLRRKLK"
BASE COUNT         5219 a         3009 c         3232 g         4629 t
ORIGIN      
        1 cgtccaattt cgtaaaacaa ccgtaacggt tgtgtaaaaa tatcatttta gagcataaaa
       61 aaaaggagtg gatttctcca ctccaaaaaa gtctaacttt tgggggtcac tacagtgtca
      121 cagtcctttt tttagtaaaa ggatacattt cttccttgta tattccatgc gtgttcgtga
      181 gaaagcatat ataatttaat taacagatag ttagggggag aaaaaatgat tttttcaatt
      241 acaatgaaac tggtgttcgg gttgatcggt ttattattag tggtacgttt gcttgggaag
      301 aaatccatgt cagaaatcac accgtttgat ttagtctata cgcttgtttt aggtgggatc
      361 ttagaagaat caacctatga tgacaatgtc catgtagggc atgtcctatt tgccattgcg
      421 ctttgggcag taatgattta tggtatcgaa cgtatcgtcc aaaaaaatga aaaggtcaat
      481 cgttgggtca aaggcgagcc atctgttcta atcaaagacg gcgtcatcaa catgacagag
      541 ttgacaaata accatatcga gatggagcag ttgcgagcaa tcttaagaca gcaagaatgc
      601 ttttctcttg aaaatgccaa acatgttatc ctagagaatg ctggtcaaat gagtgtattg
      661 aaaaaatcag atgaagacaa agctttatcg atcctgcttg tagatgaagg gcaaattcag
      721 cataaagtgc ttcagtccaa tcaattgact gaagcatggc ttatggagaa cttgaagaaa
      781 gaaggctatg cagatgtaaa acagctgatt tatgttgaat ggtcagaaga aaaagggctc
      841 tacatactaa ataaagaaga cgtcatgcat gaatcttacc gaatagatgg atagatagac
      901 agaggttgtg aaatcagcgc cttggcttgt tactcaaaac taagcgactt tttcacaacc
      961 tctgatatct aatatacgtt gttccagaag ttgcttcttc gaaaaataag ccaaaattcc
     1021 acaaaaattt aagaagcaat tttcgaaaaa tcctttctta ttttttgaag ttgatcactt
     1081 ctgtcacaac ctctcatgtc acaaaccatt tttttgtttc ctttaactca atataccgta
     1141 cgtcttccca caatatcggc tgatggtcaa tcataaagta atcttcatag gcttcaccaa
     1201 tgaagtgccc ttcgacctca tccaagagtc ttccaaattc atcttttaaa ttgagctgaa
     1261 tgattactgc tttacgagat aaaaaggctt ctgtcagtat actatcgatt tcttgcaatg
     1321 tcatttgttg tttaggaaga ggctgtttgc gtgcttcttc ttcatttttt ttgataccgt
     1381 tgactagttc ttccatcgca aaagcagtcc cccattttaa tccaaatggc cgatctcgat
     1441 actcgttata gtcaatgaat ttttttgctg tccgtctcat cttttccacc ttctaatcca
     1501 ctcataccac ctgcatgccc gccaacgaga gaactacgtg cgatggcgcg tcctccttgc
     1561 aagagactat gcgcatggat gattgattga aagccgtatt tttttctaat cgtatcgaca
     1621 acaaaatcaa ctttcgcttc atttatctgc ttttcaggat cttcaaaaag gttaagctgt
     1681 aaacctgtcg cgtatacgag gtcggaacag ttcacgccta cacgccgaat atcctggtag
     1741 tggtaatgct ggtggaaaat ggtcaataaa gcatctgcta gatctctgct agcatttgtc
     1801 gcttcgattt tcatttgctg gtgaaacccg gtcttgccat ttggatcaag atagcctaaa
     1861 gagtatccta cccataagct gactacttgt gtttttgctg cagatcgtct caagcgagtg
     1921 ccgacttggt cggccatttc tttgataacg atctcgattt cttctcgtct tgtataatct
     1981 ctatctagga tttgactatt tccgatactt tttgattttg gtgtatattc ttgtcccaaa
     2041 aaacttcgat cgatgcccca agcatgggca taaagctgag taccgatgac ccctagcttg
     2101 cttttcaagt gataataatc cgcgtgagct aaatcataaa cagaatgtat acccatttgt
     2161 ttcagacgtt ttgctgttcg tttgccgatt ccccaaaagt cagtgatatc tgagattttc
     2221 cataactttt ctggtacatc ttcataacgc cattcagcaa acatttgctt tccgtctttt
     2281 gagccattgt ctaaggctag tttagcaagt agcgggttat cgccaattcc tatagtcgtg
     2341 tagattccgg tctgctcaaa cacatcttta tggatcagtt tagccatgtc gtacgcattt
     2401 gttttaccaa agagtgccat gctatttgtt acatcaagga agctttcatc gacggaatag
     2461 acatgatggt tcgcttcgtc tgcatatttt ttatagatag aattgatttc tctgttcttt
     2521 tccatataat aggccatcct tggggcaaca atatataaat cacttggata ggggaaggga
     2581 agatctctgg cacggctgat attggtgatg ccgtaagctt tttttgctgc aggactggaa
     2641 gccaagatta atccgctacc ccgtgtttct gtcgaatcgg acggatagga catgactact
     2701 aatttagttg taaggggatc aagatccttt tcaacgcact ctacggaggc gtaaaaagat
     2761 ttgcagtcaa tacataaaat atctcttgaa ggttcttttt cataaacaaa taccggattt
     2821 tgattcgccc acataacaat cacctcttca acacaattag aacacacgtt ctctttccgt
     2881 gtcaataaaa aacagcaatt ttcaaatacg agcaaaaggc tcacatttgg aaattgctga
     2941 gaggttattt ttatctattt gtttttagct gagatcgttg atttttttat caaacatgaa
     3001 cagcttctgg atcgatcttt gttgtcccat ttccaatgtg tgcaacataa aattccggag
     3061 catagccgac aactttcatg tattcatcat agacattgtt tttgaaggca ggtatatttt
     3121 cttctttgac aagagcaatc gcacagccac caaatccagc tcctgtcatt cgtgcaccta
     3181 agacaccttc ttgtttttga gcagcatcta ctagtgtatc taattcgatc ccagttactt
     3241 cataatcata gcgaagagag tggtgagaat catttaacag ttgaccaaat gcttctaagt
     3301 tcccagcttc taattcagct tttgccttca aagtccgctg attttcagaa acggcatggc
     3361 gagctctgcg tatcaatgta tcatcgccga tttggtcaat agttgattca aattcttctt
     3421 catctaagtc tcctaaagaa gagataggca aagttttttg caaacgtgcc aaggcttctt
     3481 cgcattcgct acggcgttca ttgtattttg aatctgccaa ttcccgccgc ttattcgtat
     3541 tcatgatgac gattgcataa ccatctagtt tcactggaac catttcgtat ttcagcgtat
     3601 ttgtatctaa taagattgca tgatctagct caccgaagcc gatggcaaac tgatccatga
     3661 tacctgaatt gacaccgatg aattcatttt ctactttttt acctgtttgg actagttcca
     3721 agcgatctgt ttttaaatca aacagatctt ctaatacaac acctactaat aattctaatg
     3781 aagcgctact ggaaagtcct gcaccattag ggattgttcc ttccactaac aactcaaacc
     3841 catgatcgat tgagtgtcca gcttctttta atttaaggac cattcctttt acgtaatttg
     3901 cccagtttgc ttttttatca tattctagct catctaaagt aaaactaatg acaccgatat
     3961 cgtcaaagtt tgtggaataa actaatattt tattatcgtc tcgaggtgct gctacacctt
     4021 gcgttccata agtgatggaa gcggggaaga catggcctcc attgtaatct gtgtgttcac
     4081 caatcaaatt gatgcgtcct ggtgagaagt attgtgtagt ttttttaggt ccgaatgttt
     4141 cggcaaattt tgtgtttaag cttcttttga tttccatgat gagttattcc tttcttgttt
     4201 tgattttgaa ttctgtcact gtttcaaata ctgatccagc ttttaaatga atcgaaccaa
     4261 atgctgagaa gcgctctgca cctggagctg tttgagtctc aaatgtgatg ccaccgtgat
     4321 gtgccagttt tcggttgcgc atttctggtg tttctgtgcc gaaatttgca gtgaaaataa
     4381 ccacactaga agcatctgta gcaataccta cttggattct tttatcgggg ctagtcaagc
     4441 gagcggcttc tttacccaag ccggtttctt ttaagaaaaa tggatgatcg attccgtcaa
     4501 caagattttt ttgatccaaa tcactagcaa aaacggacgc taatttttta ggtatttgaa
     4561 aatcgaaagc acttcctgca gcattttctt ttacccctat aggaatacta tctgttcgca
     4621 aaggagcata agcttcgcta ttgagccaaa gctcatgttg atcgattgac tgagaagcat
     4681 ctcccgtcaa gttgaagtaa acatgatttg tcggattgaa taaagtatct tggtcactgg
     4741 tgccgcgaga ggtcactcgc cagatattat cttttgtcaa agtatagcgg atttccacat
     4801 ccattgtacc tggaaaacca tgttctccgt cgggacttgt agtagtaaaa atgacagaag
     4861 cttcattttc gccattcaaa atgacataag accatttttt taattcaaat ccaggagcac
     4921 caccatgcag attatgtcca gtttttggat ctgtggctaa ttggtaagtt tttccattga
     4981 gagaaaagcg gccattttcg attctccctg cggtgcgacc gatagaagca ccgatatacg
     5041 gatctttttc gatatattct tcagcggtgt caaatcccaa aactaattct cgatcatttg
     5101 attttaaact gacgattcta gcacctaaat cagagataga aagtgctagc ttgtttttgt
     5161 tctccaaggt tatcaagtga taaccttggc caaaatcttt ttctgtaatc ttcattttca
     5221 agatcctttc ttaatgggaa ggaactactg catcgtgaag ttcatcttcc attgtatcag
     5281 ctggagccaa tttgttttcc aattcctcaa cgatttgtgc atgttttttc tctgtcaatg
     5341 taatcttcca agcaaaaata atagcggaga aaatcatcaa agcagcagga gcatagaatg
     5401 cataagcttt gaaagtcgtt acgccatgag cagtgatatc agcagcagta gcattaccag
     5461 tcatcccaga agcgatagcg acaaacccta cgatcccgtt agacaaagca ccagcgattt
     5521 tatctagtaa agggcgaatt gatagagtaa cagcttcgtt acggatacca tttttccatt
     5581 gaccatattc aacagagtct gtgatcgtca tcaaagcgga taagaaaatc aattgttgag
     5641 ggaagtagaa caatactagc ccgatgataa cgattggcaa acttgaacct gcgatcgtaa
     5701 agaaaaagta agaagaaatc atcattgcga tccctccaag gtaaacgtag cgacgagaga
     5761 tgacgcgtgt aagcattggg aatagtggaa cagctactaa accaagtaag gcagcaacta
     5821 gaccaacgat ccaaaattca gttggttggc caagtacata tttgaagaag tagaataata
     5881 cggcagttgt tgctacattg gcaatcgcat aagcgatata agcaagggca agccacatca
     5941 actgatcatt ttgagcaact gctttgaaca catctttgat cccagctttt tcattttgct
     6001 gacggatcgc agtatcgact tcttttgtgc ccaaggcagt gataatcgca gtaccgcctg
     6061 aaataacagc agcaatcaca ccgaaccaga accagcctga agcgccttca atatgtttat
     6121 gagtaacaag ataggtaaag aaagtaacga tagggacaac gactagagta gtaccatttg
     6181 cacctagaga agagccgaaa cgagcaaacg tagcaaattt ttcacgttcc tttgtttccg
     6241 tactcaaagc tggaatcatt gaccaaaaag cgatatcctt aaatgaataa aaactatcca
     6301 ataagatgaa aacgattgca aataatatga taaataaagt cgtattatga gtagctaagc
     6361 caaagaaatt ggtaaagatt gctaccaaga gtacggaact aacaacgccg ccgataacta
     6421 accatggttt gaatttaccg tattttgtac gagtattatc gatgatgcca ccaatcaaag
     6481 gatcaaaaat aatttcaact aagcggatac ctacaactaa gcttgtaact gtagcaatca
     6541 tttttgcatt cgttgcctta tctgttcctg cgaacatctg gcttgtcacg aataccataa
     6601 aataagtgct taatgtaaca tagaatgtgt cgtgtccaaa agcgcctaaa gcgtaggaca
     6661 cacgttggat cattttgctt ttcataatct gtattcctct cctgttcaat atataatact
     6721 aagtaaaccg gttacaacga ataacaaaga tcttttaatg acttaaactt aacaagaaat
     6781 cgcttccttt ttaatatatt tctgtgactt ttatttaact ttttgttgca ttatactaaa
     6841 ggagtaagga gggatagacg tggagcaatc gattttttta aaaaaggaac atcaacaagt
     6901 agaaaaagaa ttgtattttg atttttgtgg tttctctaaa acgatgccgt ttcattcttt
     6961 tggtcctgct attcgcaatg attacatcct gcatgtcgtg atggaaggca aaggatctta
     7021 tcatgtgaaa aatcaacaat tccaattgaa aaaaggtgat ctgttcttga ttcgtccagg
     7081 agactcgacg ttttacttat cagatggaga agatccgtgg atgtattgct ggctttcatt
     7141 tggtgggagt gctgcagaag aaatcattcg gcagtcgctt ttcagagaag accaatacac
     7201 gatggtctct tctgacatac aaaaatatat cgacattatt ttagaatgca tgaaagatac
     7261 ggaagaaacc ttggaaaatg aattacagtt aacagcatta acctaccgtt ttttatcagt
     7321 ccttttaaaa gatggcggaa aggtcagcgg tggtgtacaa aaggttcatt cctctttagc
     7381 tgttgaaaca gtcaaataca tcgaggcaca ttatgctgaa aaattgtctg ttgaggaaat
     7441 cgcccagcat ttagcggtca atcggagcca tttgtcgcga gtattcaaaa atcatatggg
     7501 taccagtatc aaggaatatt tgatcggggt acggatcaat cgaggggcgt ttcttctttc
     7561 tctgacggat gaatccgtgg aaagtattgc ctatcaagta gggttcaata gtctagtcgt
     7621 atttagccga atgttcaaaa agagtaccgg agaaacggct acgaactatc gtaaacgtat
     7681 gaaaaacgaa aattttaaga atattgcttt ggataccttg aaaaaacagt tggaaaaaca
     7741 aaccgcagtt tcttggacaa cctgattagt ctggttaaaa tgacaagcat cggcaaaaga
     7801 aacttgtggc ggttatgcta cactgtaaag cgaagactaa aaaggaggaa aagggatgcg
     7861 tacacttatc atcattagcc atccaacgat cgaagaatct ttaaatcagc aatttttcaa
     7921 agaagcttct gctggtttac cagctacttg gcatcatttg gaatcaagtt atccagacgg
     7981 aaaaatagat atagagactg aactacagct cttggcccag catgatcgga tcattttcca
     8041 gtttccgttt tactggtata gttctcctgc tcatttaaaa atttggcaag ataatgtctt
     8101 ggaacgagca gcacatgtat taaaagggaa agaactagga atcgtattga caacaggtgt
     8161 ggcggaaaaa gaataccaag ctggaggaaa agaagaatat acgatttccg aatttttgag
     8221 gccgtatcag cgaatcgcaa ataaattcca tatgacctat cttccgccat tcgttttagc
     8281 acaatttatg tatttatccc aggaaaaacg ttgggagaag ctaattgctt atcagcagta
     8341 tctctcattg gaaggcaaac catcgctgac tcagcggatt gactggttta ttcaaagagt
     8401 acaggaaaat cagaaaatgc aggaagaaga atctgaaaaa caagcatata tcgtagaagc
     8461 actgactgag gcaaaagagc agatcgaaga cttgaccttt acactgcaag aaatgaaagg
     8521 aacttcatta tgacaaatga ttggctgatc caacaattag atgaattaag cgaaaaatca
     8581 gaatatcaag accgtgcgct cttatatgaa ataaagaaac tgatggctga gatagacgaa
     8641 cgaacaatgc agctgcaagg agaaatcgac ggacaatctt ggaatcatct caactggtga
     8701 aattttctta taaataacaa agataaaaat gtgcacagcg acaattattt gctacaataa
     8761 gaatatgaag cgtttgataa cgctattata cataaaaatt ggtggtgaaa aaaatgtatg
     8821 atgaaaatga gtggggagaa aatgttcgga tgacccgaag aagacagcaa aaacaggcag
     8881 ctcgtaagcg gatgatcatg attggggcag cagttgccgt gtttttgatc atagcaggag
     8941 gagttgcggg tctatatttt catggaaaaa attcgtcaga agcgaaaagt acgaccataa
     9001 cggaaacaac gacaaagaaa acagagacga aacaaacaag tgagtcatca actaaaacaa
     9061 aagagacaca aacttctgaa gaaaaaacag caaatggttt gccagagcaa cttcaagcaa
     9121 tcgtaaatga ttattcgaaa aagatcgaag aaaagacgcc tgcattgatt gaagaatacc
     9181 agacagaaat tcagggaaat caagagggaa tagctggttt atcagcagtt gctaaccaaa
     9241 aagcacgtga gcttcaagca atctccgacg aagggatcag aaaattacgt gcagcgtatc
     9301 aggcagcaga aaataaagac ggtgtagact tagatacttt gatcaatcaa ttatcggcta
     9361 attatacgaa tcatgtcgct aagatttccg atatttattt gcagactagc gcctctcttc
     9421 aagcagagtc aacaagcaca caagacacaa catcttctga ttctgagaca gcagagtcgg
     9481 tagaagcttc tgaaatgcaa gctcgaacaa cacaagacac aacagattcc tctgaaacta
     9541 ataccaacag cgcgacttca caggctgcga caaccgtagt tttggaagga gaaggtccaa
     9601 atcaaatagc cgaacgaact ggcgtaccag tagaaacgat cttgtcgctg aatgggatga
     9661 gcatggagaa ttatttcctg actcccggtg agacgctaaa attgaattga taaaagcaat
     9721 aaaaaagaac gaagcttcca ctaaggtgaa gcttcgttct ttttttattt tccggttctt
     9781 ttttcgcgta caaagtaatg gatcgtcatg taaagcaaga aaacgagtcc aataatcaat
     9841 gagattctcg tttcaggatt gataaacatg aaaatcaata cgatagctaa aaaagcaagt
     9901 gtcacataat tcgtataagg agatagtggc attttaaaag gatgatcatg cattttatct
     9961 gcgtgcttct ttttgaagcg aatctcgctg atcaaaatca ctacccaagg aaccatacca
    10021 ggcaaaatac ttgagctgta tacgaaaacg aaaatatttt ctgactggtt gatgaacatt
    10081 ggtagaagat aatttaagat cagaccaatt aagattccac ctgagattgc tacgacagga
    10141 taaaaaggcg caccgtgacg agatagtttc agaaattttt tagacatctt tccatttatg
    10201 gcaagtgtat agaccatacg acttgcgctg aaaatccctg aattgcaacc tgataatgcc
    10261 gctgtaatca cgacaaaatt aatcagtcct gcagcaaatg taatgccgac tttagaaaaa
    10321 gtttgtacaa aaggtgaacc aatctcactc aattgattcc acggatagat actcacaatg
    10381 ataaaaatag caccaatata aaaaatcaag attcgaccga tagtggactg gacagcttca
    10441 atgatcgtct ttttaggatt ctctgcttca ccagcagtca ttccgatcag ttcgattcct
    10501 tgataggaag caacaacgat cgataatgca aagaaaaatc ctttgacgcc tccagtgaag
    10561 aatccaccat ttttccaaag atttgaaatc cctactgcct gaccatgatt accaaatcca
    10621 aataagatta ccccaagacc tgcaatgatc atcaaaatga tcgttatgac tttgatgatc
    10681 gaaaaccaaa attctaattc gccaaacgcc ttgacagaaa taagatttgc cagcataaga
    10741 aacaagacga caatgattcc gggaataatt gccggcaaat caggccacca ataattcata
    10801 taagatccta cagcaatcac ttcacttatt cctacgacaa tatattgaaa aatattactc
    10861 caagcggtca agtagccagc cacaggatgg atgtactcgc tcgcgtaatt tgcaaaagaa
    10921 ccagtcgaag gatcaacgta aagcatttcg cctaaagcac gcataatcaa atacaaaaag
    10981 aatcctgcaa tcgcataggc tcccatcact gagggccctg tccaacgtat ggttgaggag
    11041 gcacccataa acagaccgac accgatcgtt ccacctagtg cgatcatttg gatatgacgg
    11101 gcactaagac ttcgagttaa cttttcttct tccatacaaa aactcctttc gttgttgagt
    11161 caacaagaaa aaataatcaa acgtttcaac acatgttttt cattattagg ttagccttat
    11221 tgaactcttg atgtgattct acagaaaatt ttcataaaat gcgaggaaaa ttcgaaaaaa
    11281 actttttcat cattctactt gaaaatgctt tccattttag ttaaactata tttaaataaa
    11341 aagttaggta aacctaaaaa ggagtgtaag atttggaacg tacggccatt gtaattcaag
    11401 acctttcagc cgcttatcaa gggaaaactg ttttgaaaac tatcaacttg acgataaatc
    11461 cacaaaaaat taccggtatt atcggaccaa acggtgcagg aaaatcaaca tttatgaaat
    11521 ctttgctaga gttgatacca gctactactg gtaaagtcac cttttctgga cagccggtaa
    11581 actcagtccg taaaaaaata gcctacgtgg aacagcgaag tgaattggat ctttcctttc
    11641 caattgatgt attaggcgtt gtacttttag gaacatatcc atctttacga attggacaaa
    11701 gacctgggaa aaaagaaaaa gaacgtgcaa gacaagcttt gaaaaaagta gggatggaag
    11761 aatatgccaa aagacagatc agcgaactat cgggtggaca gcttcagaga gtttttattg
    11821 caagagctct agcccaagga gcagaatgga tctttttaga tgaaccattc gtagggattg
    11881 atgcgttaag tgaacgaaag atctttgaca tcttgcagga attgaagaat tcaggaaaaa
    11941 cgattttgat cgtccatcat gatcttcata aagtagaaga atatttcgat gaggttattc
    12001 ttttaaataa acagctgatc gcttccggtc cagtacaaga agtctttaca tcgaagaacc
    12061 ttcaattggc ctatggtgag atcatcagac atttagtaaa aggaggagag aaaaaatgat
    12121 ccaatccttt attgatggat taatgaatta tcaattcctg caatacgctt tggtcacttc
    12181 catgcttgta ggacttgctt ctggagtcat cggttctttt attattttac gagggatgtc
    12241 tttgatggga gacgctatct ctcacgcagt tcttccaggt gtagccattt cttatatgtt
    12301 tggttttagt tacatctttg gagccactgc ttttgggatg ctaacagcag cagtgattgg
    12361 tttcgtgact cagcggagcc gattgaaaaa tgatacagcg attggaattg tctttagctc
    12421 cttttttgca ttagggatta tcttgatttc atatgcccag agtgcgacag atttgtatca
    12481 tattttattt gggaatgtct tagctgttag ggaaagcgat ttgttgctga cagcactcgt
    12541 tagtggaatc gtcttgatat ttgtcttttt cttttataaa gaattgaaga tcacatcatt
    12601 cgatccgaca atggcaaaag cttatgggct gaacacatcg ctgatccatt atcttttgat
    12661 gttctttttg acattagtgg ctgtagtcag tttacagaca gtaggaacaa tcttggtgat
    12721 tgccatgttg atcacaccag ccgccacggc ttacttgcta acgaaccatt tactgaaaat
    12781 gatcattaca gctgcaggaa tcggtatgct aagtgcagtt gtcggtgtgt ttttcagtta
    12841 tagttacaat tggccatcag gagctacgat cgtgttagca tgtaccgcat tttttatcct
    12901 tgctttttta ttttctccaa caaaaggaat tttatttcag aaaggaagca gatagatgaa
    12961 aacaaaaaaa tcactctttt taatcttggc agttagtttc ttggtattag ccggttgcgg
    13021 aaagcaggct tctgaccaag cggatgaagg aagtaaagaa aagttgtcag tcgtggctac
    13081 caattcgatc ttggcggaca tggcaaaaga ggtcggaaca gatcaaatag atatccacag
    13141 tatcgtacca gtcggaacag atccgcatga atatgaagta ctaccagaag acatcaaaaa
    13201 ggcaagcgat gcagatgtta ttttatacaa cggtttgaat cttgaaacag gtaacagctg
    13261 gttcgataac ttgatggaaa cggctaaaaa agaagaagga aaagattatt ttgcagttag
    13321 caaaaatgta gaacctcttt atttaactag cggtgaagaa catacaaaag cagatcccca
    13381 tgcttggcta gacctatcta acggaataaa atatgtggag gaaatcgcac gtatattctc
    13441 tgaaaaagat gcagaaaatg cgacactcta taaaaaaaat gcagaagcat atgtggaaaa
    13501 actaaaagaa ttagatacac aagcgaagga aagttttgct tctatcgaag agaacaaaaa
    13561 attattagta acaagtgaag gggcattcaa gtatttttca cgagcatatg atttgccagc
    13621 agcttatata tgggagatca atacagaaag tcaaggtacg cctgatcaaa tgaaagcgat
    13681 cattgatcag ataagagcaa aagaagtacc ggttttattc gtggaaacca gtgtagactc
    13741 aagaagcatg gaacgggtag caaaagaaac gggtttgaaa atctatgata aactgttcac
    13801 tgattccata gcaaaagaag gagaacaagg agattcttac tatcagatga tgaaatggaa
    13861 tattgaaacg atccatgaag gtttgtcaca aacaaaagaa agctgaaaag tgcaaggtct
    13921 agcttgagga gcagaccttg cattttttat aagagatatg aaaaaaaatt atgctgtttt
    13981 tcgtcgtaca gatacctttt ttgaagaaat tccgttatag ttagagtaag gaaggaaggt
    14041 gtggctatga attttacacg taaagataaa tatacgatta aagaagaagt ctttcgattg
    14101 atcagccagc aggaaaatac cgacaaggat caagaccttc aatttcttga agcagaatta
    14161 gctaaattat tcatagcaaa tcaaaatgag acaacagaac cagatgaaga gctgtacata
    14221 ggcaaatggt tgtttgatga atttcagcct ttagatgaaa ataccggtat cggttatttt
    14281 gtgttccttt ctccagtcgg cttcacacct ggcgtattag ttaaacaaaa cgacgaaata
    14341 accgtcaaag catggcctgc attattttca gtcatcaaag cagaaggagt aatcaaaaag
    14401 atccagtcgg cctattataa aagtcaattt attgccaaca gcaaattaat gaatatgttt
    14461 tataatcgtc tagtagaaat tgctaggcct gggaaaatcc cgcaaacatt cacgtacaca
    14521 cagcaaaaaa atctctatga agaagtattg tattcccgcg atctgtctga gttttcgcca
    14581 tgggaagagg taaggctcca tgaactgtta agtacaaaac cagtttcatt gaattttttt
    14641 aatgcattag ataacaaaag aaaattaatt tcaacaaatt attgggaagt atttgctgca
    14701 agaaaaaaat ggatggaaga aaagaagatt gccgatcagc agatccagtt tgtccagctg
    14761 aaaaaagaaa aactatatta tgggttatac agccgttaac agcaagcgag gaagaccatg
    14821 acattaaaag aagaaatcat acaagaaagc aaacgattag gaattgacaa aatcgggttc
    14881 accacggcgg aaccttttga ttcattaaaa gaatcgttgg aagaacaaaa agcagctggc
    14941 catacttctg gctttgaaca tccaaatatc gatgagcgtc tttatccaga aatgacattt
    15001 gatcagccga aatcgatcat ttccattgca cttgcgtatc ctactaggat ccatgaagaa
    15061 gtgcctaggg atgaaaaacg cgggcagttt gctcgtgctt cttgggggat cgattaccat
    15121 gatatactca gagaccgttt gaaccgattg atcgaattca tccaaacaag agcagaaaaa
    15181 tggcaaaaag aagaggaatg gcgtctagct ccgcaagtag atactggaga actgatcgat
    15241 gtcgctgtag cacagcgagc tggtttagga tttattggac gaaacggatt gttgatcact
    15301 gaagagttcg gttcatttgt ctatcttggt gagatcgtta ctaatattgt atttgagcct
    15361 gataaaccag gagaatttgg ttgcggtgac tgtatgcgct gcgtgactgc ttgtccgaca
    15421 aaagcattgc ttggtgacgg aagaatgaat gcccagcgat gtttgtctta tcaaactcag
    15481 acaaaaggga tgatgccgga agaatatcgt aaaaaaatgc gaaatgtcat ttatggatgt
    15541 gatatctgcc agttagtctg cccttacaat caaggaaaag attttcattt tcatgaagag
    15601 atggaaccaa aagtagacga agtatatcca aaattaaagc caatgctttc gatgtctaat
    15661 aaagacttca aacagcaatt tgggcattta gctggttcat ggcgtgggaa gaaacctttg
    15721 cagagaaacg cattgattgc tttagctaat ctaggtgatc gaacggcatt gcctgagatt
    15781 tcagaatgtt taacagatgt ccgccctgtc atcagaggaa cagcagcttg ggctattgga
    15841 cgtctaggaa caaaagaacc ggaaaaatgg cttgatgtat tagatgaaat attagctaaa
    15901 gaaacagaag aagtcgttat aaacgaattg gaacatgccc agaaattatt gcggagaaag
    15961 ttaaaataaa aaaatagtta acattattca aagtttttgt ccagtaattg ttgtaacaat
    16021 tactggtttt tttgtatttt ttacattttt ttaagagaat ttgataaaaa tcaaaaattt
    16081 ttttgcttt
//
LOCUS       DACDKM010000063        16035 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20834_2016
            SAMN12640370-rid9891663.denovo.063, whole genome shotgun sequence.
ACCESSION   DACDKM010000063 DACDKM010000000
VERSION     DACDKM010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640370
            Sequence Read Archive: SRR10040891
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16035)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16035)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742835.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742835.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:04:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,012
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 29 of 75
            Pseudo Genes (incomplete)         :: 33 of 75
            Pseudo Genes (internal stop)      :: 19 of 75
            Pseudo Genes (multiple problems)  :: 5 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16035
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640370-rid9891663.denovo.063"
                     /isolate="E20834_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(26..466)
                     /locus_tag="IV334_001794"
     CDS             complement(26..466)
                     /locus_tag="IV334_001794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HAP8943859.1"
                     /translation="MMIKLYTGSGCSSSRRAKRWLIVHDIPFIEQNLIRQPLTKNEFI
                     HLLKLTNNGVTEMLSTHSISYKNLVHRIDELSLSELYIEIRTSPSLLKKPIIYDQSAK
                     LLTGFSEDKIRMFLSKTKRKQELNELLLSGHLVDFKENNLFAMS"
     gene            626..1132
                     /locus_tag="IV334_001795"
     CDS             626..1132
                     /locus_tag="IV334_001795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HAP8943860.1"
                     /translation="MSNTADAIVVIDLQKGVCFDSSPIHELETVTALVNKRIKTYETI
                     GKPVIFVQHEDAQLVPQTKPWELLSELSIPEHSYFVRKTHANSFYKTNLSVLLTELDC
                     HSIELCGAQAEYCIDTTVKFAHGLGYQLMMCRGATTTYDNPFMKAKETIAFYEKIWDK
                     RFLTFLEQ"
     gene            1300..3213
                     /locus_tag="IV334_001796"
     CDS             1300..3213
                     /locus_tag="IV334_001796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HAP8943861.1"
                     /translation="MNSLRTKQVLKGALVILFAVSTSVPQWTVYGESISSESESREVE
                     TTETTDAGNETKSSTSQTETNTETTVETSVTSTDQETTATTDNQQTEEATATKMSNDE
                     SSSAKVSDKENGTENDDIGKGENFNGKEYLNYFGKQMTEILNGPTEDIKSKLIELLVT
                     ELAVNDYGVDKEIIKHLIATYLLYDIAFAKNSVLMSMLGGTDDGTTPTENTGFYPNTK
                     QLHQTVLNKQDGKMMDVYAYYVDQGSDKTVMIHGGFRGNWNNGIVTEEYNDFYKAGYN
                     LLFVDSRATGNSGGDYVTYGQYESDDVLYWINQEVRERPSQKILLYGGSMGAATMMSV
                     LAKDIPVNVKGIIENCGFASIDEQLRFTYSQTVAPALPDAIKNQLDIIGDQEHEDLFM
                     GLLKQYYFDQEMHLDTKAALPTIGMSDSLPKLIIHGTADDVVPVSNAQKLYELSGGYK
                     DLLLVEGAGHGKAQEVDHAAYTKHVTDFLKVVFHDQINVKYVDENNKSLLKDQDEIQL
                     YGAYGENYMTEQKTFEGYELANVEGPTEGIFNETIPTIIFYYKKIPVAPPKKQDPTEN
                     ADNKGKNEPREDQSSDDTQPIQMTVKVQKKTDAKKRMLPKTGEKKHSLLIYSGGFLSV
                     STIITWIWRRKRG"
     gene            3218..4219
                     /locus_tag="IV334_001797"
     CDS             3218..4219
                     /locus_tag="IV334_001797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAP8943862.1"
                     /translation="MKWFKRIVIAVPVIIVVILVTVFLINQITPKPISLLVRKQFDTS
                     EKEQVYARPDDFQQKIEQIDIKKEISYPSKYKNNNMDIYTPKVEKKKLPILFWMHGGA
                     YVGGDKNDCRDYLEYLCSDTQQIIVNIDYERSPEAKHPTPVIQLNEAIQAAKKEIKDQ
                     ADWSNISIGGNSAGAQISGEYLLALQNEEIKKADHLDPTLENKQITKFISLSGLLDPS
                     ALTQVSDKISSFLYEKCGWAYFDNKDFEQSEKVKSLALVRHADRWTQNTFLTDGNTNT
                     FTKQMEEVTSVFEKAGVKVTKVDYAEKNAVLNHEYQFDFSNKQAQETYQKLVMFFKE"
     gene            complement(4421..4627)
                     /locus_tag="IV334_001798"
     CDS             complement(4421..4627)
                     /locus_tag="IV334_001798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3955 domain-containing protein"
                     /protein_id="HAP8943863.1"
                     /translation="MKAIHISLLFFASALILFAIHLCSQPYINNNGILVEPAFFCLPL
                     GFLSLLASAGFGFVAFFKRSKQQI"
     gene            complement(5273..6121)
                     /locus_tag="IV334_001799"
     CDS             complement(5273..6121)
                     /locus_tag="IV334_001799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HAP8943864.1"
                     /translation="MSVSIRLQIKTMYNDFSPKEQAIADYILENPSKVSHSPISNLAS
                     ELGIADSTFFQFTKKLGYNGFKDFKMAMLIQENDLSAISIHENIQKTDTELTMAQKVF
                     ESSISTLNDTKKLLQQKDLKKAATIINESKRVYFFGVGGSEIVATDAYHKFLRSPIAT
                     SHSTDYHIQLMEASLLTEKDCAVLISHTGQSKETIHIAETVRKTGAKTIVITSQANSS
                     LAKLGDVVFISISEETEFRSEALASRISQLSILDSLYVILMFYNEKESRRSIAKVRHT
                     ISEIKE"
     gene            6285..7187
                     /gene="gnd"
                     /locus_tag="IV334_001800"
     CDS             6285..7187
                     /gene="gnd"
                     /locus_tag="IV334_001800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="decarboxylating 6-phosphogluconate
                     dehydrogenase"
                     /protein_id="HAP8943865.1"
                     /translation="MEIGVIGLGKMGLNLALNLKEHHHKVQGLDISSTAVSQARENDI
                     PAYQEISEFLATFEQKKIIWLMLPAGEITENMLRKLFPKLGPGDIIIEGGNSYYKDSI
                     RRAEEFQKNEIGYLDCGTSGGIEGARHQACLMIGGDKETFLSVEQLFKDVAIERGYLY
                     AGASGSGHFLKMIHNGIEYGMMQAIGEGFQLVQKSEYAFDLEQVARVWNHGSVIRGWL
                     MEIMEDQLNEHPNLSNYRGIVSASGEAKWTVETALEMAVPVPVIATSLFMRNLSQEED
                     SFSAKVVSALRNGFGGHEIVEKGE"
     gene            7189..8190
                     /locus_tag="IV334_001801"
     CDS             7189..8190
                     /locus_tag="IV334_001801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacid dehydrogenase"
                     /protein_id="HAP8943866.1"
                     /translation="MPDYHIAIVNSSSFGRVFPHHLERLKSVGDVHFFTVDQEIPGKE
                     LAELLKGFNIIIASVTPFFRRDFFENKDELLLISRHGIGYNNIDLKAAKEHQTIVSII
                     PALVERNAVAENNITNLLSMLRRTTQAQARVKDDRWEDRAEFVGRTLFNKTVGVIGAG
                     NTGSGVIEILRNGFRCDVLAYDPYKSDLYIESFGAKKVELQELLEQSDIICLCANLTE
                     ENYHMISDKEVDQMKENVYLSNSARGALVDEEAMINGLKSGKIAGYATDVLEEEPGRQ
                     DHPYLAFEQVVITPHISAYTRECLEAMGEKCVRDVEQISQHLLPERSVQTKSNYIKG"
     gene            8187..8471
                     /locus_tag="IV334_001802"
     CDS             8187..8471
                     /locus_tag="IV334_001802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HAP8943867.1"
                     /translation="MMKKMVNGLKVKTGPQFYLYEEGGISKVSDLLKSYGAKRVLVTH
                     GTVSWEKALPKLVFLNDETIQFFYHRYSGECSYAEARRIATIIKKMKSIS"
     gene            8504..9301
                     /locus_tag="IV334_001803"
     CDS             8504..9301
                     /locus_tag="IV334_001803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HAP8943868.1"
                     /translation="MGYSANLARIDFGVIPTLASNCAPWTPLAVMYKENGQAEGKTEH
                     FYRQAAFFITDPQLVIDSPVPYFIAGLADTLAKWYESELILEQDFLQDESFLKLAQDT
                     AKICKEEILNHSAKAIQDMAQRKLTSEFKQLSEIIFAVAGLVGGLGDKYARNVAAHAM
                     HDAISKYIPESHRFLHGEKVAYGIFYQLALEGRWEKIDELYPLYRELELPVSLEQMGL
                     FPKDKQIIDKMAEFIDSKEKVHLIPIEVTQKSLIQSMMELEGYIRKI"
     gene            9315..10148
                     /locus_tag="IV334_001804"
     CDS             9315..10148
                     /locus_tag="IV334_001804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAP8943869.1"
                     /translation="METLSVVQSLLIALWVAAVMSRWLGGGATLTLRFSPLMTGLVVG
                     LIMGEVAQAMIVTAALQMIYMGVFSPGGSMPAEPSIAAAIAVPVALLGDLSPEAAIAV
                     AVPVGLLGSYLYQFRFFINTFLGKYTDKAVEDLNTKAMARSIILYPTVASFLLFVPLV
                     FIALYLGAPLIADVIKALEGTVVIHILEVVGGGLAAIGIATTVYVIGRKDYLVFFFLA
                     YFMSVVLKSLDITMVTYAIFGIIIALIFVQSQKGKQVQPATSNNNSASFDDEDDYDDG
                     F"
     gene            10166..11023
                     /locus_tag="IV334_001805"
     CDS             10166..11023
                     /locus_tag="IV334_001805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HAP8943870.1"
                     /translation="MAEIMDNQTGAPEEITKKDVTKAYLRWHFANEIPHSFERYLAPS
                     LLYGMMPILKKLYKDEDSLKAAYKRQLLFFNTQLSWGGGVITGLMSSMEQQRAEEEYE
                     EKEILMQDDLMYNTKAGLMGALAGIGDAIDSGTVQYIFIAIAVPWAQQGSPLGALFPF
                     ICFGLYQVLLGVFFARQAFTMGRNATGLKQNTGVQTAIEMLSVLGLFMMGVLAGNYVK
                     VESSLKFAISGREFVIQEILDQIVPGILPLAVVMGVYLFYTKKGLKVTQALLWLTGIL
                     IVLAGIGIL"
     gene            11045..11554
                     /locus_tag="IV334_001806"
     CDS             11045..11554
                     /locus_tag="IV334_001806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAP8943871.1"
                     /translation="MGVVNLARVDERLIHGQVMVTLSQKSGVNSIFVVDEVVAKDKFM
                     RDLYKNAGSRTGQKTIVITPEKAKFYWDEYQFKEYNCILIAKTVQTIYDLVKHGIPME
                     ELNIGGIAQKNPDTDLLVTKSVYLNKSDAEKLKEMNEQYGVKNIYFQATPSAPKTGLK
                     DVLNKFDLS"
     gene            11591..11992
                     /locus_tag="IV334_001807"
     CDS             11591..11992
                     /locus_tag="IV334_001807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8943872.1"
                     /translation="MKKNELFRDWEFRYRYIYRKRRTKKSKQRFLSALVSDIYSMRTD
                     VTVIAYDTLAYRSKNIYVGDIEKAEKVICTYYDTPVHALGSYFMFDWKDQRKKTIYSI
                     LLSFILLFSLGWWGMMIYNKNPHHVFDLLSV"
     gene            12104..12457
                     /locus_tag="IV334_001808"
     CDS             12104..12457
                     /locus_tag="IV334_001808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8943873.1"
                     /translation="MLEMIRTIDDPSVAYAFVDEGCYGEKGLDSVRSGMKKEAILFYL
                     DSVGADTPLQFSGNYFSNKEQWLKQVDKLKEKNVNYIFSARKKQAQFFYLTKTDLRGK
                     TFNWQNANQIIALFR"
     gene            12491..12934
                     /locus_tag="IV334_001809"
     CDS             12491..12934
                     /locus_tag="IV334_001809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HAP8943874.1"
                     /translation="MLEIMIVTHGKLSEGLKDAAEVIVGTSSHIETMCLESGEDIQTF
                     GEKVKEKLTMINTAEPMLVFVDLISASPYNQALLAVNELTEEKKAATYVIGGVNLPML
                     LEAINHQMLHTPIEEAVEQIVKQGKDNLTVWHASAEEKKLDEDDF"
     gene            12951..14036
                     /gene="uxuA"
                     /locus_tag="IV334_001810"
     CDS             12951..14036
                     /gene="uxuA"
                     /locus_tag="IV334_001810"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HAP8943875.1"
                     /translation="MKWGFRWYGEKGDSIPLKYVRQIPGINGIIGTLLTKMPGDVWEI
                     SEIEELKASVESQGLELLGIESVAIHDAIKAGTDERDYYIDNYIRTIKNLSRCGVRLI
                     CYSFKPIFGWAKTDLFNENNDGSYSLLYDQKIVDQMEPGEMYQLIHSQSKGFKLPGWE
                     EERLKKFAQLEKLYEGMTEEELFENLTYFLEKVIPVCEKADVKMAIHPDDPPWELFGF
                     PRVTKNLGDLKKILNAVDSPYNGLTLCTGSLGANPENDLVEIIHEVGSRIHFVHFRNV
                     EFLGERKFKETAHPSTEGSLDMYHIMKALVQEGFDGPIRPDHGRTVWGEVAMPGYGLY
                     DRAMGLTYMQGLYEAISKEKPLGEHYE"
     gene            14029..14742
                     /locus_tag="IV334_001811"
     CDS             14029..14742
                     /locus_tag="IV334_001811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HAP8943876.1"
                     /translation="MNKIKLFIFDMDGLLLETGRLAYRAYVKSAQKYDYEMRKEVYYL
                     LTGQTEMAIREQMGILYGEDVPYIKWREAINQYKEKIVKEDKRVYTKKGAEEILSFAK
                     ERGIHTIVASSNTRVKVEMYLKMENLYALFDQIISGDDVKKGKPEPEIFLKACSKMNI
                     PPSEALVFEDSIAGIEAARRAGILSFLIKDHLIGLPDHNGKHKLKVDVSLYESNKADY
                     EFYDLHQAKRFLESKGLYL"
     gene            15494..15694
                     /locus_tag="IV334_001812"
     CDS             15494..15694
                     /locus_tag="IV334_001812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HAP8943877.1"
                     /translation="MNNGTVKWFNADKGFGFITGEDGNDVFAHFSAIQGDGFKTLEEG
                     QAVSYDVEDGQRGPQATNIVKA"
BASE COUNT         5554 a         2493 c         3389 g         4599 t
ORIGIN      
        1 gaaaagcaga ttgtttatgc caaaattaag acattgcaaa aagattattt tctttaaaat
       61 cgacaagatg accagaaagc aagagttcgt tgagctcttg ctttctttta gttttagaaa
      121 ggaacatcct gattttatct tcgctaaatc cagtcaaaag ttttgcagat tggtcgtaga
      181 taatcggttt tttcaataaa gaaggggatg tacgaatctc aatatatagt tcactcaaac
      241 tgagttcatc tattctgtga acaagatttt tataactaat agaatgagta gagagcattt
      301 cggtcacgcc gttgtttgtc aattttagta agtggataaa ttcatttttg gttagtggtt
      361 gacgaattaa gttctgttcg ataaacggta tatcatggac gataagccaa cgttttgcgc
      421 gtcttgaaga cgagcagcca gaacctgtat acaatttaat catcatacat tcctccaata
      481 gctaagtatt tatgaaataa taacatgaag aaatagtcga aaagtgccat ttagaggtgt
      541 aaaataagaa aatgttgtca cgtataatgg aactagctag ataagaaata ctattaaatc
      601 aagaaaaaag aaagaaggca caagtatgtc aaacacagcc gacgcaatag tagtgatcga
      661 tttacaaaaa ggggtatgtt ttgattcttc gccgatacac gaattagaaa cagtgacagc
      721 attagtgaat aaaagaatca aaacatacga aacgattggg aaaccagtta ttttcgttca
      781 acacgaagat gctcaattag tgccacaaac aaaaccatgg gagctattgt cggaattatc
      841 tattcctgag cacagttatt ttgtgagaaa aacccatgcg aattcatttt ataagactaa
      901 tttgagtgtt ttattaactg aattggactg tcattcgatc gaattatgtg gggcacaagc
      961 agagtattgt atagatacga ctgtgaagtt tgctcatgga ttaggatacc agcttatgat
     1021 gtgtagagga gcaacgacaa cttatgataa tccttttatg aaagcaaagg aaacaatcgc
     1081 tttttacgag aaaatttggg ataaacgatt tctgacattt ttggagcaat aagttgtttc
     1141 ttcgtttaaa aaaagcagaa tggatactgg tttatccatt ctgctttatt acgtaaaatg
     1201 agaaaataat cctgttctat ttttatggga taaaccattt attttttgta taaaaaatgt
     1261 ttgcgtttac atataattct cctagaaaca taaaaaggag tgaatagctt gagaacaaaa
     1321 caagtattaa agggagcgtt ggtcatctta tttgctgtaa gtacaagcgt cccgcaatgg
     1381 acagtatatg gagagagcat ttcttccgaa tcggaatctc gggaggtaga aaccacagaa
     1441 actactgatg ctggaaatga aacgaaatcg agtacttcac aaacagaaac aaatactgaa
     1501 acaacggttg agacatctgt tactagcaca gatcaagaaa cgaccgctac cacagataac
     1561 caacaaacag aagaagcaac agctacaaaa atgtcaaacg acgaatcctc ttctgctaaa
     1621 gtaagtgaca aagagaatgg gacagaaaat gatgatatcg gtaaaggtga aaatttcaat
     1681 ggtaaagaat atttgaatta cttcggtaaa cagatgacag aaatacttaa tggaccaaca
     1741 gaagacatca agtcaaaatt aatagagtta ttagtcactg agcttgctgt taatgattat
     1801 ggtgtagata aagagattat caaacatctg attgctactt atctattgta cgatatcgct
     1861 tttgcaaaaa acagtgtgtt gatgtcgatg cttggaggta cagatgatgg gacaacgcct
     1921 acagaaaaca caggcttcta tccaaataca aaacaacttc atcaaactgt actaaacaaa
     1981 caagatggca aaatgatgga tgtatatgca tattatgtcg atcaaggttc agataaaaca
     2041 gtcatgatcc atggcggatt tcgagggaat tggaataacg ggatcgttac agaagaatac
     2101 aatgattttt ataaggccgg gtataattta ttatttgttg attccagagc gactggtaat
     2161 agcggaggcg attatgtgac ctatggacaa tatgagtcag atgacgtcct ttactggata
     2221 aatcaagaag ttcgagagcg gccatctcaa aagattttac tttatggtgg ttcgatggga
     2281 gcagctacga tgatgtctgt attagcgaaa gatattcctg tcaatgtcaa aggaattatt
     2341 gaaaattgcg gatttgcatc aattgatgaa caactacgtt ttacgtacag ccagacagta
     2401 gcccccgcac taccggatgc cataaaaaat cagttagaca tcattgggga tcaagagcat
     2461 gaagatttat ttatgggcct tttgaaacaa tactattttg atcaggagat gcatttggat
     2521 acaaaagctg ctctacctac gattggaatg tcagattcat tgcctaaatt gattatccat
     2581 ggaacagcag atgatgtagt acctgtgtct aacgcgcaaa aattatatga gttatcagga
     2641 gggtataaag atcttttatt agtagaagga gcagggcatg gaaaggcaca ggaagttgac
     2701 catgctgcct acacgaaaca tgtaacggac tttttgaaag tagtgttcca tgatcagata
     2761 aacgtaaagt atgtggatga aaacaataaa agtctactca aagaccagga cgaaatccag
     2821 ctttacggag cttatggaga aaattatatg acagagcaaa agacattcga aggatacgaa
     2881 ttagcaaatg ttgaaggacc gactgaaggg atttttaatg agacgatacc aactattatt
     2941 ttctattaca aaaaaatccc agtagctcca ccaaaaaagc aagatccgac agagaatgca
     3001 gacaataagg gaaagaatga acctcgagag gatcaatcaa gtgatgatac gcaaccgatt
     3061 cagatgacag tgaaagtaca aaaaaagaca gatgcaaaaa aaagaatgtt gccgaaaact
     3121 ggagaaaaga aacattcgct tctcatttat tcaggtggat ttttatctgt tagcacgatt
     3181 atcacatgga tttggcgaag aaagagaggg taaatgaatg aaatggttca agcgaatagt
     3241 gattgcagta ccagttatca tcgtagttat tttagtcact gtatttttga ttaatcaaat
     3301 cacaccaaag ccgattagct tgcttgttag aaaacaattt gacacaagtg aaaaagaaca
     3361 agtatatgcc cgaccagatg atttccagca aaaaatcgaa caaatcgata taaaaaaaga
     3421 aatatcctat ccttcaaagt acaaaaataa taatatggat atctacacac cgaaagtgga
     3481 aaagaaaaaa ttaccgattc ttttctggat gcatggaggc gcatatgtag gaggggataa
     3541 aaatgattgt cgtgattatt tggaatatct atgttcagac acgcagcaaa tcatcgtaaa
     3601 tatagattat gaacgatcac cggaagcaaa acatcctaca ccagttatcc aattgaacga
     3661 agccattcaa gcagctaaaa aagagataaa agatcaagca gattggtcga atatttcgat
     3721 tggtggaaat tcggcaggtg cacaaattag tggagaatat ttgttagcac ttcaaaatga
     3781 agaaattaaa aaagctgatc atcttgatcc gactttggag aacaagcaga ttacgaagtt
     3841 tatttctttg tctggtctgt tggatccttc tgcacttact caagtaagtg ataaaatctc
     3901 tagctttctt tatgaaaaat gtggatgggc ttactttgat aataaggact ttgaacaatc
     3961 agagaaggtc aaaagtttag ccttggtcag acatgcggat cgttggacgc aaaacacatt
     4021 cttaacggat ggaaatacaa atacgtttac gaaacaaatg gaagaagtaa cttctgtatt
     4081 tgagaaagcg ggggtgaaag tcacaaaagt agattatgcg gagaaaaatg ctgttttgaa
     4141 ccatgaatat cagtttgatt tcagcaataa acaagcgcaa gaaacatatc aaaagttagt
     4201 aatgtttttc aaggagtaaa gtcttatgat gcaaaagagg ttgtaatctg tcacttagct
     4261 ccaagtcaca aggagcaatt tcaataaaca agcttttcat ggtttatgaa tattgtaact
     4321 tgtgactgag gagttagaga tggcaagggg caccttttat tcgaaatttg ggaatatcac
     4381 acgattattg tcacgatctc ttttacgtaa attttataag tcagatttgt tgttttgatc
     4441 gtttaaaaaa agcaacaaat ccgaagcctg cggaggcaag aagggacaaa aagccgagtg
     4501 gtaagcaaaa gaatgctggt tccactaata ttccgttgtt attgatataa ggctgggaac
     4561 aaagatgaat agcaaataaa atcaatgcac tagcgaaaaa aagcaaagaa atatggatag
     4621 ctttcataag cagcacctcc ttttttataa ttgtatgata aatagattaa gaatctataa
     4681 atgtgacaag tttgttagga tgtgaataaa atgatcatct agcgaacttg aaacaaagat
     4741 ggctaatgat aagcaattaa tgattcagca atcagatagg tacaaagttt accatcatac
     4801 gtctagacca ataatagaaa atatgggaat ataatagaaa agatttagga tacccccaaa
     4861 agaatttccc caaaaattcg atgtatccta aactttttta ttttacataa caatcatatc
     4921 aactgtgaaa taaaattaca aatgataacc taaaatgagg ccaaaagcca gtgttatcaa
     4981 ggaaatactt aattcaccaa aatttttttt aattttttag aaaataagaa acaaaaataa
     5041 taaaaaaaag aaaagataaa ttttttacta ctgtatacta actttgttat cgcatctaac
     5101 aagtaactcg aataatacca atacaacctt ctccaaagat gcgatgacat acgttctaac
     5161 ccaccgacta ttgatgttct gtaccaccct tgcagaacca aaaaagccac taccaaatat
     5221 ttggtagtgg ctttttttgt tttatggatt tttcgctttt tctgttatgg tgctattctt
     5281 tgatttcaga aatagtatgt cgaactttag cgatagaacg acgtgattct ttttcgttgt
     5341 aaaacatcaa aatcacatat agagagtcca aaatgcttag ttgagaaata cgggaagcta
     5401 aagcttcaga tcggaattct gtttcttcag aaatagaaat aaaaacgaca tcgccaagtt
     5461 tagcaaggga agaattagct tggctagtaa taacgattgt ttttgctcca gtttttctta
     5521 cggtttcagc aatatggatt gtttcttttg attgccctgt gtgagaaatc aaaacagcac
     5581 agtctttttc cgtcaataaa gaagcttcca tcaattgaat atggtaatcc gtgctgtgag
     5641 aagttgcgat aggggaacgc aaaaatttat gataagcgtc agttgctaca atctctgaac
     5701 cgccaacacc gaaaaaatag acacgtttag attcattgat aatagtagcc gcttttttta
     5761 gatccttttg ttgtaacaac ttttttgtgt catttaaagt tgaaatacta gactcgaaca
     5821 ccttttgcgc catagtgagc tcagtatctg ttttctgaat attttcgtgg atagaaatag
     5881 cagatagatc attttcttgg atcaacatgg ccattttaaa atctttaaat ccattataac
     5941 caagtttttt tgtaaattga aaaaaagtag agtcagcaat gcccagttca cttgctaaat
     6001 tgctaatagg actatgactt actttgcttg gattttctaa aatataatcg gcgatagcct
     6061 gttcttttgg gctgaagtcg ttgtacattg ttttgatttg tagtcttatg gagacgctca
     6121 tgagaattcc tcattttcat aataagatta tcattttttc cgttctcatt aatgttaaag
     6181 atacaaggag agtaggaaaa aatcaaacaa aaaatatttt tgtaaaatct atttacaaaa
     6241 aaattttttt gtataatgag ggtataagaa atggaggcgc tatcatggaa attggtgtaa
     6301 ttggtttggg aaaaatggga ttgaatttag ctttaaactt aaaagagcac catcacaaag
     6361 tacaaggact cgacatttca agtacagctg ttagtcaggc aagagaaaat gacattcctg
     6421 cttatcaaga gatctctgaa tttctagcta cttttgagca gaaaaaaatc atctggttaa
     6481 tgcttcctgc tggagagata accgaaaaca tgttaagaaa actttttcca aaactcggac
     6541 caggagacat tattattgaa ggcggaaact cttattataa agattcaatc cgtcgagcag
     6601 aagagtttca gaaaaatgaa atcggttact tggattgtgg gacttctgga ggaatcgaag
     6661 gagcacgaca tcaagcctgc ttaatgattg gaggagataa agaaactttt ctatccgtgg
     6721 aacaattatt caaagatgta gcaattgaaa gaggatatct gtatgctggt gcatccggaa
     6781 gcggacactt cttgaaaatg atccataatg gaatcgaata tggaatgatg caagcaatcg
     6841 gtgaaggatt ccaacttgta caaaaaagtg agtatgcttt tgacttggaa caagtcgcac
     6901 gtgtttggaa tcatggttcg gtgattcgcg gctggctgat ggaaatcatg gaagatcagt
     6961 taaacgaaca tcccaattta agtaattatc ggggaatcgt ttctgcatca ggtgaagcaa
     7021 aatggacggt tgaaacagcg ctagaaatgg ctgttccagt tccagtaatt gcaactagtt
     7081 tgttcatgag gaatctttca caggaagaag atagtttttc agcaaaagta gtttcagcac
     7141 taagaaatgg gtttggcgga catgaaatag ttgaaaaggg tgagtaaaat gccagattat
     7201 catatagcaa tcgttaattc cagcagtttc ggaagagtct ttccacacca cttggaaaga
     7261 ttaaagtcag ttggagacgt tcatttcttt actgttgatc aagagattcc aggaaaagaa
     7321 ctagcggagt tacttaaagg attcaatatc attattgcaa gtgttacgcc attttttcga
     7381 cgagattttt ttgaaaacaa agatgaactg ctattgattt cccgacatgg tattggttac
     7441 aacaatattg atttaaaagc agcaaaagaa caccagacaa tcgtatctat catacctgct
     7501 ttagttgaac gaaatgctgt agcagaaaat aatataacta atttgttgtc catgcttcga
     7561 cgcacgacac aagcacaagc aagagtcaag gacgatcgat gggaagatcg ggcggagttc
     7621 gtcggtagga cgctgtttaa caaaacagtt ggtgtgattg gtgcaggaaa taccggaagc
     7681 ggagtaatcg aaattttgcg taacggtttt cgctgtgatg ttctagctta tgatccttac
     7741 aaatcagatt tatacatcga aagctttggt gcaaaaaaag tagaacttca agaattactg
     7801 gaacaatcag atatcatttg tttatgtgcg aatttgacag aagagaacta tcacatgatt
     7861 tctgataaag aagtcgatca gatgaaagaa aatgtttatc tatctaatag tgcaagaggc
     7921 gctctagttg atgaagaagc gatgattaat ggtctgaaaa gcggaaagat cgcaggatat
     7981 gccacagatg ttttagaaga agaaccggga cgtcaagatc atccgtattt agcgtttgaa
     8041 caagtagtga ttacgccaca tatatcggcc tacacaaggg aatgtttaga agcaatgggt
     8101 gaaaaatgtg tcagagatgt cgaacagatc agtcagcacc ttcttcctga acgttctgtc
     8161 cagacaaaga gcaactatat aaaaggatga tgaagaaaat ggtgaacggt ttaaaagtga
     8221 aaacaggccc tcaattttat ttatatgaag aaggtggtat ttcaaaagtt tctgatctct
     8281 tgaaatcata cggcgcgaaa agagtactgg tcacccacgg aacggtatca tgggaaaagg
     8341 cgctacctaa attagtattt ttgaatgacg aaacgattca atttttttat catcgttatt
     8401 ctggagaatg tagttatgct gaagccagac gaatagctac aatcatcaaa aaaatgaaat
     8461 cgatttcgta gttggcgttg gcggaggaaa actgaccgat ctagtgggtt atagtgccaa
     8521 tcttgcaaga atcgatttcg gagtcatccc tactttagct agtaattgtg ctccatggac
     8581 acctcttgct gtcatgtata aggagaatgg acaagcagaa gggaaaacag aacactttta
     8641 cagacaagct gcttttttta taacagatcc ccaattagtg attgattccc ctgttccata
     8701 tttcattgcc gggttagcag atacactagc aaaatggtat gaatctgagc tcatcttaga
     8761 acaagatttt ttgcaagatg aatcattttt gaagctagca caagatactg cgaagatctg
     8821 caaagaagag attttgaatc attctgctaa agcaatccaa gatatggcac aaagaaaact
     8881 gacatcggag ttcaaacagc tttcagagat tatctttgct gttgctggtt tagttggagg
     8941 attaggagat aaatacgcaa gaaatgtggc agcacatgct atgcatgacg ctatcagtaa
     9001 atatattccg gaaagccatc gttttctaca tggagaaaaa gtagcgtatg ggattttcta
     9061 tcaactggca ctagaaggtc gctgggaaaa aatagacgag ttatatcctt tgtatcggga
     9121 attagaactg ccggtttctc tagaacaaat gggattattt ccgaaagata aacagataat
     9181 agataaaatg gctgaattta ttgattcgaa agagaaagtg catttgattc ctatagaggt
     9241 aacacagaaa tcactgatac agtcaatgat ggaactggaa ggttatatca gaaagatctg
     9301 aaggaggagg acacatggaa acattaagtg tagtacaaag tttgttgatt gctttatggg
     9361 tagccgcggt tatgtcccgc tggttaggtg gaggagccac gttgacctta cgtttttctc
     9421 ctttgatgac tggattagtc gtcgggttga tcatggggga agttgcacaa gcgatgattg
     9481 ttacagccgc tttgcaaatg atttatatgg gggttttttc tccaggagga tcaatgccag
     9541 ctgaaccatc tattgcagca gctatcgcag taccagtcgc attattaggg gatctgtcgc
     9601 cagaagcagc gattgccgtt gcggtgccag tcggattgct cggaagttac ttgtatcagt
     9661 ttcgtttttt catcaatact tttttaggta aatatacaga caaagctgta gaagatttga
     9721 atacaaaagc tatggcccga tcaattattc tttatccaac ggttgcctca tttttattat
     9781 tcgttccgct tgttttcatt gctttatatt taggggcgcc attgatcgct gatgtaatca
     9841 aagctttaga gggaacagtc gttatccaca ttcttgaagt agtaggtgga gggttagcgg
     9901 caatcggtat cgcgacaact gtatacgtta tcggtcgaaa agattatctg gtctttttct
     9961 tccttgctta ttttatgagt gttgttttga aatctttaga tattacgatg gttacttatg
    10021 ccatttttgg gattattatc gctttgatct ttgtccagtc gcaaaaaggg aaacaagtac
    10081 agcctgccac aagcaataac aattcagcat cttttgatga cgaagatgac tatgatgacg
    10141 gattttaatt taggaggaat gggaaatggc tgaaataatg gataatcaaa caggtgctcc
    10201 tgaagaaatc acgaaaaaag atgtaacaaa agcctatctg agatggcatt ttgcaaacga
    10261 aattcctcac tcttttgaac gttacttggc gccatcacta ctatacggaa tgatgccaat
    10321 cttgaaaaaa ctttataaag atgaagactc tttaaaagca gcatataaac ggcagttact
    10381 gttcttcaac actcaattaa gctggggtgg cggtgtgatt actggcctaa tgtcttctat
    10441 ggaacagcaa agagcagaag aagagtatga agaaaaagag atcttgatgc aggatgactt
    10501 gatgtataac acgaaagcgg gattgatggg ggcactagca ggaatcggag atgcaatcga
    10561 ttcaggaacc gtccaatata tttttatcgc aatcgcggta ccttgggcac aacagggaag
    10621 tcctttaggc gcactttttc cttttatctg ctttgggttg tatcaggttt tactaggggt
    10681 atttttcgca agacaagctt tcactatggg ccgtaatgct acaggcctaa agcaaaacac
    10741 tggtgttcag acagcgattg aaatgctatc agttttaggg ttgtttatga tgggggtgct
    10801 tgcgggaaac tatgtaaaag tagaatcttc attgaaattt gcgatatccg gacgtgagtt
    10861 tgttatccag gaaattcttg atcaaatcgt gccagggata ttacctttag ctgtcgtaat
    10921 gggtgtgtac ttgttctata cgaaaaaagg attgaaagtc acacaagcat tattatggct
    10981 gactggaata ttaattgtgc tagcaggaat cggtatttta taaaaagaag ggaacgtgaa
    11041 taagatgggt gtagtcaatt tagcgcgtgt ggatgagcgt ttgatccatg ggcaagtaat
    11101 ggtaacgtta tctcaaaaaa gtggagtgaa ttccattttt gttgtagacg aagttgtagc
    11161 aaaagataaa tttatgagag atctttacaa aaatgcagga agccgtacag gacaaaaaac
    11221 aattgtcatt acaccagaga aagctaaatt ctattgggat gaatatcaat ttaaagaata
    11281 caattgtatt ttgatcgcaa aaacagtcca aacgatttac gacttagtaa aacacggaat
    11341 ccccatggaa gaattgaata ttggagggat tgctcaaaaa aatcctgata cagatctttt
    11401 agtgacgaaa tctgtttatt taaataaatc agatgctgaa aaattgaaag aaatgaatga
    11461 acaatatggc gtaaagaata tttattttca agccactcca tctgcaccta aaacaggatt
    11521 aaaagatgta ttgaacaagt ttgatttgtc ataaacaaat gcttagaaaa aagataaaga
    11581 gggcgaaaaa atgaagaaga acgagctgtt tcgtgattgg gaatttcgtt atcgctatat
    11641 ttaccgtaag cgccgtacaa aaaagagtaa acagcgtttt ctttcagcat tagtatcaga
    11701 tatttacagt atgagaacag atgtgacagt cattgcttac gatacgctag cctaccgatc
    11761 aaaaaatatt tatgttggag atatcgaaaa agcagagaaa gttatctgta cttattacga
    11821 tacacccgtt catgcattag gaagttattt catgtttgat tggaaagacc aaaggaaaaa
    11881 aacaatctac tctatattat tgtccttcat tttgttgttc agtttaggtt ggtggggaat
    11941 gatgatatat aacaaaaatc ctcatcacgt attcgatctg ctatccgtat aaacaggtat
    12001 cactgtacta gcatttggga gctacttttt cttattggga aaagcagctc gcggatggcc
    12061 tagcagacaa acttttattc gaaatacttc atccatatta acgatgctag aaatgatccg
    12121 tacgattgat gatccgagtg tagcgtatgc gtttgttgat gaaggctgtt atggagaaaa
    12181 gggcttagac agcgtgagat cgggtatgaa aaaagaggct attctttttt atctagactc
    12241 tgttggggca gatacaccgc tacagttttc gggaaattat ttttcaaata aagaacagtg
    12301 gctgaaacaa gttgataagt tgaaagaaaa aaacgtcaat tatattttta gtgcaagaaa
    12361 aaagcaagcg cagttcttct atttgaccaa aacagattta cgagggaaaa catttaattg
    12421 gcaaaatgcc aatcagatta ttgctctttt tcgataagta aaaagtgcaa aacaaggagt
    12481 gagaagacta atgttggaaa tcatgatcgt tacacatggt aagttgagcg aaggactgaa
    12541 agatgcggcg gaagtaattg tcgggacatc gagccatatt gaaacaatgt gcttagagtc
    12601 tggggaggac atccaaacat tcggagagaa ggtcaaagaa aaattaacta tgatcaacac
    12661 agcagaaccc atgctagtat ttgttgatct aattagcgca agtccgtaca atcaagcgct
    12721 cttggcagtc aatgaactga ctgaagaaaa gaaagcagcc acttatgtca tagggggcgt
    12781 gaatctgcca atgttgttag aagcaatcaa tcatcagatg cttcacacac cgatagaaga
    12841 agcggtggaa caaattgtca aacaaggaaa agataattta actgtttggc atgcttcagc
    12901 ggaagaaaaa aaattagatg aggacgattt ttagaaggaa ggctgaaaag atgaagtggg
    12961 gatttagatg gtatggtgaa aaaggggatt cgatcccatt aaaatatgtt cgccaaatac
    13021 cgggaataaa tgggataatt ggaactttgc tgactaaaat gcctggagat gtttgggaga
    13081 tttcagaaat tgaggagttg aaagcatctg ttgagagcca aggactggaa ctgcttggaa
    13141 ttgaaagtgt agcgatccac gatgcaatca aggcagggac agatgaacga gattactata
    13201 ttgataacta tatacgaaca atcaaaaact tgtctcgatg tggggttcga ttgatttgtt
    13261 atagcttcaa accaattttt ggatgggcga aaactgattt gtttaatgaa aacaacgatg
    13321 gaagctattc tcttttatac gatcagaaaa tcgtcgatca gatggagcca ggagaaatgt
    13381 atcaattgat acacagccag tcaaagggat tcaaattgcc gggttgggaa gaagaacggt
    13441 tgaaaaaatt cgctcagctg gaaaaactgt atgaaggaat gaccgaagaa gaattatttg
    13501 aaaatctgac ttatttttta gaaaaagtga ttccagtgtg cgaaaaagca gatgtaaaaa
    13561 tggctatcca tccagatgat ccgccatggg aactttttgg ttttcctaga gtgacgaaaa
    13621 atcttggcga cttgaagaaa attctaaatg cagtagattc tccatataat ggactaacgc
    13681 tgtgcacagg atctttaggg gcaaatccgg aaaacgattt agttgaaatc atccatgagg
    13741 taggttcgcg catccatttt gtccacttca gaaacgtgga gtttttagga gaaagaaagt
    13801 tcaaagaaac ggcacatccc tctacagaag gctcccttga tatgtatcat attatgaaag
    13861 cgttggtgca agaagggttc gacggtccta tacgccctga tcatggtaga accgtgtggg
    13921 gtgaagtggc tatgcctgga tatggcctgt atgatcgtgc aatgggactc acttacatgc
    13981 aaggtctata tgaggcgata agtaaggaaa aaccactggg agagcattat gaataaaatc
    14041 aagctcttca tctttgatat ggatggcctt ttattagaaa ctggacgttt agcttatcgt
    14101 gcttatgtaa aatcagcaca aaagtatgat tatgaaatgc gtaaggaagt ctattattta
    14161 ctaactggcc aaacagaaat ggcgataaga gaacaaatgg gcattttgta cggagaagat
    14221 gttccatata tcaaatggcg agaagcaatc aatcaatata aagaaaaaat cgtgaaagaa
    14281 gataagagag tatatacgaa aaaaggagca gaagaaattt tatcttttgc aaaagaaaga
    14341 ggaatccata caatcgtggc ttcgtctaat acacgggtaa aagtagaaat gtatttaaaa
    14401 atggaaaatc tatatgcctt gtttgatcaa atcatttcag gggatgatgt aaaaaaagga
    14461 aagccggaac cagagatctt tttgaaagct tgttcaaaaa tgaatattcc tccaagtgaa
    14521 gcacttgtat ttgaagactc gattgcaggg atcgaagcag caaggcgagc gggcattttg
    14581 tcttttctca tcaaagatca tttgataggg ttgcccgatc ataatgggaa gcataagttg
    14641 aaagtagatg taagtttata tgaatccaat aaagcagact atgaatttta tgacctgcat
    14701 caagcaaaac gatttttaga aagtaaaggg ttgtatctct aataaagagg actattttct
    14761 agtcctcttt atttattgtc ctgcacgact gagggaacag atgaaaaaga agtaattttt
    14821 tattttgaag tatctacctt atttgtagtg aacaatttag aacaaacaca aaacgcatct
    14881 actgttatct accagtagat gcgtttaaag agaagaacga gaatcgtcca tatgagggtt
    14941 tccactcctt atgcagattt ttttaattct tgcactgctt gttcgatcgt aacacctgtc
    15001 gctacatgat tggtattttc tttgtcaaaa acgacaaata gattcaaatc tgtatccaag
    15061 gttactcgat attgtatatc tttgttatag aatgtcatag ccattctcct ttctttaaat
    15121 acttgctgaa aggcaacgaa cgcgttgata acgaaattcc atttataagt ataccaccaa
    15181 ttgaaaggtt ttcatagtta agaaatttta aagtcaaaag ttgcagataa aagcaaataa
    15241 acaagaaaac aaaaagtgaa tcaatcgcgg ttattcaact ttttcttcga ttcgaagaca
    15301 atagaagaca atgaatttta tatttcattg cattatcctt gtgttattag aaaaagcatg
    15361 atatattaaa tgagtaatct attttgaaac gtaatttgag cgatatatct acatagataa
    15421 aactcctttt gcctatcaaa tatttattgc aacgaaaaaa cgtacattga tacgtatcag
    15481 gaggaattta tttatgaata acggtacagt aaaatggttc aacgcagaca aaggttttgg
    15541 ctttatcact ggtgaagatg gaaatgatgt atttgcacat ttttcagcta tccaaggtga
    15601 cggcttcaaa actttagaag aaggccaagc agtgtcttac gatgttgaag atggtcaacg
    15661 tggccctcaa gcaacaaaca ttgttaaagc ttaataaaaa ttgactttta accgtctttt
    15721 cgaaagaaaa gacggttttt ttgtacgaaa aaattcattg gtcgttgaat gggctgaata
    15781 gtagtgtata aaacaatttt gtggagcgtg aagcttttat ggtagaggaa aatacgaata
    15841 aagtgatgtt gataaaaaga ttcctatctt gaatatgaat cgagatgtct tgaaaatgaa
    15901 tgagtttgac caatcattat cctatcgaaa tttacagttt cgttcgattg gtctgtcaat
    15961 taatgaaaaa gtaaaaaaga atatgttcta ggaagagggc ggcacatcta aatttttaag
    16021 taacggacag ttcaa
//
LOCUS       DACDKM010000064        15948 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20834_2016
            SAMN12640370-rid9891663.denovo.064, whole genome shotgun sequence.
ACCESSION   DACDKM010000064 DACDKM010000000
VERSION     DACDKM010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640370
            Sequence Read Archive: SRR10040891
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15948)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15948)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742835.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742835.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:04:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,012
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 29 of 75
            Pseudo Genes (incomplete)         :: 33 of 75
            Pseudo Genes (internal stop)      :: 19 of 75
            Pseudo Genes (multiple problems)  :: 5 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15948
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640370-rid9891663.denovo.064"
                     /isolate="E20834_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(387..809)
                     /locus_tag="IV334_001813"
     CDS             complement(387..809)
                     /locus_tag="IV334_001813"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HAP8943878.1"
                     /translation="MDQYLTVNVVTPAGLVYDHHAAIVVARTTAGELGILPKHAPIIV
                     PLTIDEVRVKRTDSDTHVDWIAVNGGIMEVRDNVVSIVADSAERERDIDVSRAERAKQ
                     RAERQIEEAKAKQDTNELKRATVALHRAINRIKVSKHS"
     gene            complement(823..2229)
                     /gene="atpD"
                     /locus_tag="IV334_001814"
     CDS             complement(823..2229)
                     /gene="atpD"
                     /locus_tag="IV334_001814"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HAP8943879.1"
                     /translation="MSSGKIVQVIGPVVDVEFSLDQSLPDINNALVVYKNDENKSKVV
                     LEAALELGDGVIRTIAMESTDGLQRGMEVIDTGKAISVPVGKETLGRVFNVLGDTIDL
                     ETPFPEDAERSEIHKKAPAFDELSTSTEILETGIKVIDLLAPYLKGGKVGLFGGAGVG
                     KTVLIQELIHNIAQEHGGISVFTGVGERTREGNDLYYEMKDSGVIEKTAMVFGQMNEP
                     PGARMRVALTGLTIAEYFRDVEGQDVLLFIDNIFRFTQAGSEVSALLGRMPSAVGYQP
                     TLATEMGQLQERITSTKKGSITSIQAIYVPADDYTDPAPATAFAHLDATTNLERKLTE
                     QGIYPAVDPLASSSSALAPEIVGEEHYKVATEVQHVLQRYRELQDIIAILGMDELSDQ
                     EKVLVSRARRVQFFLSQNFNVAEQFTGLPGSYVPVEETVKGFREILEGKYDDLPEEAF
                     RSVGRIEDVVEKAKTLGY"
     gene            complement(2252..3154)
                     /locus_tag="IV334_001815"
     CDS             complement(2252..3154)
                     /locus_tag="IV334_001815"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HAP8943880.1"
                     /translation="MGASLNEIKTRIASTKKTSQITRAMQMVSASKLTKSEASSQKFQ
                     VYATKVREIVTHLTATQLNDIASENPRGDINYNSMLLSRPVKKTGYIVITADGGLVGG
                     YNSSILKQTMSILEEDHQSPDDYVMIAIGGTGADFFKARGINLAYELRNLSDQPSFDE
                     VRKIVNMATTMYQNEVFDELYVCYNHHINSLTSQFRVEKMLPISDLDPEEATTFEQEY
                     IFEPSKEEILSQLLPQYAESLIYGAIVDAKTAEHAAGMTAMKTATDNAANIIDELTVS
                     YNRARQGAITQEITEIVAGASALE"
     gene            complement(3168..4724)
                     /locus_tag="IV334_001816"
     CDS             complement(3168..4724)
                     /locus_tag="IV334_001816"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HAP8943881.1"
                     /translation="MAIKAEEISALIKEQIENYQNVLSVEEIGTVTYVGDGIARAHGL
                     ENAMSGELLEFSNGSYGMAQNLESNDVGIIILGDFESIREGDKVKRTGKIMEVPVGEA
                     LIGRVVNPLGQPIDGLGEIVTDKARPVEAMAPGVMQRKSVNEPMQTGLKAIDALVPIG
                     RGQRELVIGDRKTGKTSIAIDTIINQKGQDMICIYVAIGQKDSTVRTQVETLKKYGAM
                     DYTIVVNAGASQPAPLLYIAPYAGTAMGEEFMYNGKHVLIIFDDLSKQAVAYRELSLL
                     LRRPPGREAYPGDVFYLHSRLLERAAKLSDDLGGGSMTALPFVETQAGDISAYIPTNV
                     ISITDGQIFLESDLFYAGTRPAVDAGLSVSRVGGSAQIKAMKKVAGTLRLDLASYREL
                     EAFTQFGSDLDAATQAKLNRGRRTVEILKQKLHAPLAVEKQVVILYALTHGFLDSIPV
                     DSILDFEHELFEYLDTNHSDIFETIRTTKDLPEEEALNSAIQEYKDMFLATKGNNSST
                     EDKLKSIQNA"
     gene            complement(4747..5289)
                     /locus_tag="IV334_001817"
     CDS             complement(4747..5289)
                     /locus_tag="IV334_001817"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HAP8943882.1"
                     /translation="MKLDKYTVGRRYGKALFELAIDSNKAEEIYQDLVSLRQIYEQVP
                     GLGNMLSDVRLEPHEKREIMDKLVSGYDGIVKNFLEVVYAYNRMDDLLFMIEEYERRY
                     DEHQGLLLGSVTTAVPLSDEQRIQLEKNVAKTMNYQSVELKQIVDSSIIGGAIVEANH
                     RVIDGSIRTQLENMRNQLNR"
     gene            complement(5276..5800)
                     /gene="atpF"
                     /locus_tag="IV334_001818"
     CDS             complement(5276..5800)
                     /gene="atpF"
                     /locus_tag="IV334_001818"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HAP8943883.1"
                     /translation="MLNQLAIAEVGNAMLGNVIVVSGSFLILLALLKHFAWGPISDIL
                     KKREDKIANDLDSAEQSRINSAKMEQEREQQLLASRSDAADIIKNAKESGELSRQNIL
                     KDAQEEAARLKSKAQADITVERDSALNSVKDDVAELSLQIAEKILNKELSPEMHESLI
                     NQYIEGLGSSNETR"
     gene            complement(5891..6106)
                     /gene="atpE"
                     /locus_tag="IV334_001819"
     CDS             complement(5891..6106)
                     /gene="atpE"
                     /locus_tag="IV334_001819"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit C"
                     /protein_id="HAP8943884.1"
                     /translation="MNYIAAAIAIMGAAIGAGYGNGQVISKTIESMARQPEMSGQFRT
                     TMFIGVALVEAVPILGVVVALILVFGV"
     gene            complement(6159..6878)
                     /gene="atpB"
                     /locus_tag="IV334_001820"
     CDS             complement(6159..6878)
                     /gene="atpB"
                     /locus_tag="IV334_001820"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HAP8943885.1"
                     /translation="MDERSLTFHIGPIWFDGTVCIMVLLTCLIVFFLAYFFTRNLKLK
                     PTGKQNAIEWVIDFSRGIVTDNLPRKELSNFHLLAFTLFLFVFVANNIGLVTKIVLPN
                     ETTLWKSPTADPFVTLTLAFIMILLTHLFGVKKLGFKGYFVNSFLKPYSFMFPMKLIE
                     EFTNLLTLALRLYGNIYAGEVLLTLIAKMVISLGWFSLPLAIPLEMVWIAFSLFIGSI
                     QAFVFVTLSMVYMSHKIEVEE"
     gene            complement(7180..10023)
                     /gene="rnr"
                     /locus_tag="IV334_001821"
                     /pseudo
     CDS             complement(7180..10023)
                     /gene="rnr"
                     /locus_tag="IV334_001821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002418328.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
     gene            complement(10026..10772)
                     /locus_tag="IV334_001822"
     CDS             complement(10026..10772)
                     /locus_tag="IV334_001822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HAP8943886.1"
                     /translation="MRQLPKPLYAKHGKRAVLLLHAYSGSPNDVRMLARFLEKADYTV
                     YAPLFKGHGTMEPYDILQEKAESWWADTKKAIHFLQSEQFSDIAVLGLSMGGIFAVRA
                     LEEESVIGGGFFCSPLSPVKTNVPENFEKYVRQVLKTAGKSEKEINEKAVAYRSLAEQ
                     QLMDIQDQAAIVESRLSDIQQPIFLAQAGKDEMIDPNGVFETARKLSRQRVTLRWYPE
                     SGHVITVGTARRELEKDVLEFLEKLPWNEE"
     gene            complement(10884..11120)
                     /gene="secG"
                     /locus_tag="IV334_001823"
     CDS             complement(10884..11120)
                     /gene="secG"
                     /locus_tag="IV334_001823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HAP8943887.1"
                     /translation="MYNLILGIVIVLSIVMVIAIMMQPSKQNSAASAFTGGADQLFGK
                     QKARGFEAVMQRSTAVMGAIWMILLFVLAYLSSK"
     gene            complement(11238..11516)
                     /locus_tag="IV334_001824"
     CDS             complement(11238..11516)
                     /locus_tag="IV334_001824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HAP8943888.1"
                     /translation="MEIKEEKNRLALFNDEQQEIGEMTWSDAGPDIMIIDHTFVDPAY
                     RGQKLAEKLVYTGVELARRDGKKIIPLCPYAKKEFEKKPEYQDVLRQS"
     gene            complement(11629..12597)
                     /locus_tag="IV334_001825"
     CDS             complement(11629..12597)
                     /locus_tag="IV334_001825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAP8943889.1"
                     /translation="MVIFYFILILIIIGISYHFLSYFYAKKLVRAGVQRGYDWYEDTG
                     HRLMDPAAVTSVEKKRQKLEEECESFWEQGTPKTIKSYDGLKLAGQMFIHPNKQNKWV
                     ICVHDYRSTGKRDMSHIGKRYAEKGFNVLVPDLRAHGESEGEIIGMGWLDRLDLIAWI
                     QLILDEQPNASIILHGGSMGASTIMMASGEKLPSAVKGFILDSGYVSVYAEFRYMLSK
                     ITVFPKKMVMRYANHYAQKYAGYSLKQASATRQLGSNHLPLLVIHGERDHFVPTEAAY
                     TIQNATAGDKALLLVPEAEHLEASMKDPKTYWTVIFSFIEQRITMN"
     regulatory      12812..12856
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00522"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="PreQ1 riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF00522"
     gene            12880..13854
                     /locus_tag="IV334_001826"
     CDS             12880..13854
                     /locus_tag="IV334_001826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside hydrolase"
                     /protein_id="HAP8943890.1"
                     /translation="MRKVIIDCDPGIDDTLALSLAVKSPDIEVVAITVVCGNVPADIG
                     TQNVLKCLERCDRLDIPVYQGMEKPLKQPFISAQDTHGMDGLGETNFPMILRKQAEPL
                     HAVDFLADYFKEKTNTSVIALGPLTNIAFALKVNPNIGKHMDRFVSMGGTYKSHGNCS
                     PVAEYNYWCDPEAASYVFENLKQTIEMVGLDVTREIVLTPTILEYCCQMNPEEGEYLK
                     AITRFYFDFHWKQERILGCVINDPLAVAYFIEGAICEGFKSFTAVETQGISRGQTLVD
                     RYEFWQKPANSKIMTKVDTRLFFRKFLTVLLNAQEETIMKDLERLKMG"
     gene            13859..14434
                     /locus_tag="IV334_001827"
     CDS             13859..14434
                     /locus_tag="IV334_001827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HAP8943891.1"
                     /translation="MNKNKMATRVLTVMALCIGINYIGGTIALWLRLPIYLDSIGTIF
                     AGALLGPVPGMLTGLSSGILSGVTTDIFSLYYSPIQILTGLLAGLILPRYLGKDKNSL
                     KLLLLALILSVPGTIVSSIITVQLFGGITSSGSSMIVQLLHGLGLSQTTSVILVQAGT
                     DYLDRLLSIVVVSLVITMLPQRLIYSKRKIG"
     gene            complement(14501..15640)
                     /locus_tag="IV334_001828"
     CDS             complement(14501..15640)
                     /locus_tag="IV334_001828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArgE/DapE family deacylase"
                     /protein_id="HAP8943892.1"
                     /translation="MKKEEKIAILQEIIRIKSVNGNEGEVAAYLNKLLAKHDITGEIV
                     SYRDGRDNLIARYQKGQSGKVLGLSGHMDVVAAGDESSWTYAPFAAEIHGNRLYGRGA
                     TDMKSGLAAMVIAMIELKESGKPFNGTVKLLATVGEEVGELGGEQLTKAGYVDDLDAL
                     IIGEPTNYSLMYTHMGSINYTVTSHGKEAHSSMPDQGYNAINHLNEFITKANAEMNHL
                     AETIENPVLGKTIHNVTLISGGNQVNSIPSHAQLQGNIRSIPEYPNDKIIALLQSIVN
                     ELNQETDYHLELMIDYNKIPVKADPDSPLIHCIQQQFSQPLPLVGAAATTDAAEFTKA
                     NHSFDFVVFGPGVVTLPHQVDEYVEIDNYLDMIEKYQGIILSYLA"
BASE COUNT         4356 a         3443 c         2856 g         5293 t
ORIGIN      
        1 ctgagccaga atgtggttca ttctgactca gatttgattt tgcacaactc atcagtcctt
       61 attgacaaag agcccaaaag tcttgtcgca gtcccttatt ccaaataaac ggtgttcaat
      121 catctgaccc tcggtattag ctcaaaaaac ggcaaaatat gaggaactat tttccatttg
      181 tttctctaat actcaggaca aaacgctgat ttcacaacct ccgatatacg gtgttccaga
      241 agtagcttct tcgaaaaata agctgagatt tcacaaaaat ttgagaagca attttcgtaa
      301 attccttctt atgtcttgaa gctgaccact tctgtcataa cctcttttta ctgttcttaa
      361 acacggataa acatccgcat ctcattttat gagtgcttag atactttgat acgattgatt
      421 gctcggtgca acgcaacagt tgcccgtttc aattcgttcg tatcttgttt tgcttttgct
      481 tcttcgattt ggcgttctgc gcgttgtttt gcacgttccg cacgagatac atcgatatca
      541 cgttcacgtt ccgcactatc agcaacgatt gaaacaacat tatcacgaac ttccatgatt
      601 ccgccattaa cagcgatcca atcgacgtgg gtatctgaat ctgttcgttt tacacgaact
      661 tcatcaatcg tcaatggaac gatgattggt gcatgtttag gcaaaatccc aagctcacca
      721 gctgtcgtac gtgcaacgac gatagctgca tggtgatcat agaccaaacc ggcaggagtt
      781 accacattta ctgttaaata ttgatccatg gggcacccct ttctagtagc ctagtgtttt
      841 cgctttttct acgacgtctt cgattcggcc aacacttcgg aaagcttctt caggaagatc
      901 atcgtatttg ccttccaaga tttcgcggaa tcctttcact gtttcttcta cagggacata
      961 tgaacctgga agaccagtaa attgttcagc cacgttaaag ttttgtgaca agaagaattg
     1021 gactctgcgg gcacgagaaa ctaacacttt ttcttgatcg gataattcat ccatacctaa
     1081 gatagcaatg atatcttgta attcacgata tcgttgcaat acgtgttgga cttctgttgc
     1141 tactttataa tgttcttctc ccacgatttc aggtgctaaa gcacttgaag aagaagccaa
     1201 tggatctacc gctggataaa tcccttgttc tgttaattta cgttccaagt tggttgtcgc
     1261 atccaaatgg gcgaatgctg ttgccggcgc cgggtcagtg tagtcatcgg ctggcacata
     1321 gattgcttgg atcgatgtga tcgaaccttt tttcgtagat gtgatacgtt cttgcaattg
     1381 acccatttct gttgccaatg ttggttgata accaaccgca gaaggcatcc gtcctaacaa
     1441 ggctgatact tcagatccag cttgagtgaa acggaaaatg ttgtcgataa acaacagtac
     1501 atcttgacct tctacatcac ggaaatattc cgcaatcgtc aaaccagtca aagcaacacg
     1561 catacgtgca cctggtggtt cgttcatttg tccgaacacc atggctgttt tttcgataac
     1621 tcctgaatct ttcatttcat aatacaagtc attaccttca cgagtacgtt cacctacacc
     1681 ggtaaataca gaaataccac catgttcttg ggcgatatta tggatcagtt cttggatcag
     1741 tacggtttta ccaacaccgg caccaccgaa tagtccgact ttcccacctt ttaaatatgg
     1801 ggcaagcaaa tcgataactt tgatccctgt ttccaaaatt tctgtacttg tacttaattc
     1861 gtcaaaggct ggtgcttttt tatgaatttc gcttctttcc gcatcttctg ggaaaggtgt
     1921 ttctaaatcg atcgtatctc ctagtacgtt gaatacgcga cctaatgttt ctttacctac
     1981 aggaacagag attgctttgc ctgtatcaat gacttccatt cctctttgta aaccatctgt
     2041 tgattccatc gcaatggtcc ggatcactcc gtcccctaat tctaacgctg cttcaagaac
     2101 aacttttgat ttattttcgt catttttata aacaactaat gcgttgttga tatctggtaa
     2161 ggattggtct aaagaaaatt ccacgtcgac aacgggaccg attacttgaa caatcttgcc
     2221 tgaactcatc ttttttcctc caatctcaat actattctag tgccgaagct ccggcaacaa
     2281 tttcggtaat ttcttgggta atagcccctt gtcttgcacg gttgtaagaa accgtcagct
     2341 catcaatgat attagcagca ttatcagttg ctgttttcat tgctgtcata ccagcagcat
     2401 gttctgctgt tttggcgtca acgatcgcac cgtagatcaa actttcagca tactgaggca
     2461 ataactgaga caagatttct tcttttgaag gttcaaaaat atattcttgt tcgaaagtag
     2521 ttgcttcttc tggatctaag tctgagattg gcagcatttt ttctacacga aactgactag
     2581 ttagcgaatt gatatgatgg ttataacata cgtataattc atcgaatact tcgttttggt
     2641 acatagttgt tgccatattt acgattttgc ggacttcatc aaagcttggc tgatcagata
     2701 gattgcgcag ctcatatgct agattgatac cgcgtgcttt aaagaagtct gcaccagttc
     2761 cgccaattgc gatcatcacg taatcatcgg gtgattggtg gtcttcttct aaaatagaca
     2821 tcgtttgttt caatatcgag ctgttatatc caccgactaa accgccatct gcagtgatga
     2881 cgatataacc cgttttctta acaggacggc tgagcaacat gctgttgtaa ttgatatccc
     2941 ctctaggatt ttctgaggca atatcattta actgagtagc agtcaaatgc gtaacgattt
     3001 ctcttacttt tgttgcataa acctgaaact tctgtgaaga agcttcagat tttgtcaact
     3061 tagaagcaga caccatctgc attgcccgcg tgatttggct cgtttttttc gttgaagcga
     3121 tacgtgtctt gatttcattt aatgaagcac ccattcatct cacctcacta tgcattttga
     3181 atggatttca atttatcttc tgtagaggaa ttatttcctt ttgtagctaa gaacatgtct
     3241 ttatattcct gaatcgcact gttcaacgct tcttcttctg gaagatcttt tgttgtacgg
     3301 attgtttcaa agatatctga atgattggta tccagatatt cgaataattc atgttcgaag
     3361 tctaaaatac tgtctacagg gatactatct aagaatccat gtgtcaaagc atacaaaatc
     3421 actacttgtt tttcaacagc tagcggtgca tgcaattttt gtttcaagat ttctactgta
     3481 cggcgtccac gatttagttt tgcttgtgtt gctgcatcca agtcagaacc gaattgtgta
     3541 aatgcttcta attcacggta acttgctaag tctagacgta aagtcccagc aacttttttc
     3601 attgctttga tttgcgcaga accccctaca cgagatacag atagtccggc atcaaccgct
     3661 ggacgagtac ctgcgtagaa caagtcgctt tctaagaaga tttgtccatc agtgatcgaa
     3721 ataacgtttg ttgggatata agcagagata tctccagctt gtgtttcaac aaatggtaag
     3781 gcagtcattg aaccgccacc taaatcatca cttaattttg ctgcacgttc caataagcgt
     3841 gagtgtaagt agaaaacatc ccctggataa gcttcacgac ctggtggacg gcgaagcagc
     3901 aaggaaagtt cacgataagc aacagcttgt tttgaaagat catcaaaaat gatcaagaca
     3961 tgtttcccgt tgtacatgaa ctcttcaccc attgcagtac cagcatatgg tgcgatataa
     4021 agcaacggcg ctggttgaga cgcaccggca ttcacaacga ttgtgtaatc cattgcgcca
     4081 tattttttca atgtttcaac ttgtgtacga actgtagaat ctttttgtcc gattgctaca
     4141 tagatacaga tcatatcttg acctttttgg ttgatgatcg tatcgatcgc aatggaagtt
     4201 ttccctgttt tacggtcacc gataactaat tcacgttggc cgcgtccgat tggtacaaga
     4261 gcgtcgattg cttttagacc tgtttgcatt ggttcgttaa cagatttacg ttgcataacg
     4321 cctggtgcca ttgcttctac tggacgagct ttgtcagtaa cgatttcacc tagtccgtca
     4381 attggctgac ctagcggatt aactacccgt ccaatcaagg cctctccaac tggaacttcc
     4441 atgatctttc ctgttcgttt tactttatct ccttcacgaa tggactcgaa atcgccaagg
     4501 ataatgatac ctacatcatt tgattctaag ttttgcgcca ttccgtatga gccatttgaa
     4561 aattcaagca actcaccact catcgcgttt tctaaaccat gtgcacgagc gatcccgtca
     4621 cctacataag ttacggtacc gatttcttcg actgaaagca cgttttgata attttcaatt
     4681 tgttctttaa tcaaggcact aatttcttct gctttgatgg ccattcgatt cacctacctc
     4741 tttgtactat ctatttaatt gattacgcat attttctaat tgcgtgcgaa tgcttccgtc
     4801 gattactcga tggttagctt caacaatcgc gccaccaata atagaagaat caacgatttg
     4861 ttttaattcg actgattgat agttcattgt ttttgcaaca ttcttttcta attgtattcg
     4921 ttgttcgtct gaaagtggaa ctgctgttgt gacacttccc aacagcaaac cttgatgttc
     4981 atcatatcga cgttcatatt cttcgatcat gaacaaaaga tcatccattc ggttatacgc
     5041 ataaacaact tctaaaaagt ttttaacgat tccatcatag ccactaacaa gcttgtccat
     5101 gatttctcgt ttttcatgag gttctagacg aacatcactt aacatattac ctaatccagg
     5161 tacttgttca tagatttgac gtaagcttac gagatcttga taaatttctt ctgctttatt
     5221 tgaatcgatc gccagttcaa ataaagcttt cccgtaacgt cttcctactg tatatttatc
     5281 tagtttcatt tgaagaacct agaccttcga tatattgatt gattaatgat tcgtgcattt
     5341 ctggagataa ttctttgttc aagatttttt ccgcgatttg aagagatagt tctgcaacat
     5401 cgtcttttac agagttcagc gctgaatcac gttctacagt gatatcagct tgggctttac
     5461 tttttagacg agctgcttct tcttgagcat ccttcaaaat attttggcgg cttaattctc
     5521 cactttcttt cgcatttttg atgatatcag ctgcatcaga acgagaggct aacaattgtt
     5581 gttcgcgttc ttgttccatt ttcgctgagt tgatgcgaga ttgttctgca gaatccaaat
     5641 cattggcgat cttgtcttca cgttttttca aaatatcgct gattggtccc caagcaaaat
     5701 gcttcaagag agccagtagt atcaaaaatg agccgctgac aacgatgaca tttcctaaca
     5761 tcgcattgcc aacttctgca attgccaatt gattcagcat aggcgagcct tcctttcttt
     5821 gaagtacttc tagttttcat gtaaaaaagc agtaacaaac caaattgttt ctgccttaat
     5881 actgattcaa ttaaacacca aaaactagaa tcaaggcgac aactacccct aagataggaa
     5941 cagcttcgac tagagctacc ccgataaaca ttgttgtacg gaattgacca gacatttcag
     6001 gttggcgagc cattgattcg attgttttag agataacttg accgttacca taaccggctc
     6061 cgattgctgc tcccatgatt gcgattgctg ctgcgatata attcataatt gttttcctcc
     6121 taaattagat aaatagtagt ttgtttttaa aacgaatatt attcttccac ttcaatctta
     6181 tggctcatat aaaccataga taaagtaacg aagacaaatg cttggatgct tccgataaac
     6241 aaggagaatg cgatccagac catctctaat ggaattgcta acggcaatga gaaccagcct
     6301 aagcttatta ccatttttgc aatcaaggtt agcaaaactt cccccgcata aatattccca
     6361 taaagacgta aagcaagtgt taataagttt gtaaattctt caatcagttt catcggaaac
     6421 ataaaactat atggtttcaa gaacgaattt acgaaatatc ctttgaagcc taactttttc
     6481 accccaaata agtgagtaag taagatcatt ataaatgcca aagttaaagt aacaaacggg
     6541 tctgctgtcg ggcttttcca aagtgttgtt tcgttaggta aaacgatttt tgttactaaa
     6601 ccgatgttgt ttgcaacaaa aacaaataaa aacaatgtaa atgccaacaa atggaaattg
     6661 ctcaattctt ttctaggcag attatctgtt acgattcctc gactaaaatc aatgacccat
     6721 tctatcgcat tttgtttgcc tgttggtttc aacttaagat ttcttgtaaa aaagtaagct
     6781 aagaagaaaa caatcaaaca ggtcagtaac accattatac aaactgtacc atcaaaccaa
     6841 attggtccaa tgtggaacgt tagcgagcgt tcatccaatc catttcacct cttttccccg
     6901 aaattcacat aaaatcaatt catgtgcgta tctggttttt tccaatcggt ttctaaccac
     6961 acggtatcat accaccatga aacagaaaat tcaaaacatt ttcatgaaaa ttagacactt
     7021 ttttatttca gtcaaaaacg aagcgaattt ttctttatac acggtgctcc tgatctaaga
     7081 aaaaacctat gataatcatg ttttatcaga ttaaatgcta acaaatttta tcattaagta
     7141 aaagcaaaag caccgaatat gcgaaaaagg ataatgaaat tatctagaga aattttttaa
     7201 tgttcgatca atttgtcgat cgatatcttt tcgtttcaaa tcttcccgtt tatcataaga
     7261 ttttttccct ttcgccagac caatcaatac cttggcatag ccgttacgaa tatagacttt
     7321 taaaggaaca acagtgattc cagtattttt caattcattt tcaagccgga tgatttgttt
     7381 tttgtgtaat aataactttc tcgtgcgcaa cggatcatga ttaaaaatat ttccttgttc
     7441 ataaggactg atatgaacat tatgcaagaa agcttctccg ttgcggacgc gaataaatcc
     7501 atcttttaga ttgatccggc tgtttcgtat cgacttgatc tcggttcctt gcaagaccat
     7561 ccctgcttcc atcgtgtcga tgatcgaata atcatggcga gctttcttgt tttgtgcaat
     7621 taattttccc tcgccttttg gcatagctgt ttcctccaac tcattttatt ttttcttttt
     7681 cttcttttta acacctttgt aaaatggctt cttccctttt ttcttaccga acgtttgctt
     7741 actatcgcta tatcgtttgt tcttcttgcc atttctttct gggcgttttc ctgcttgttt
     7801 tccttcgcgt tttcctgatt ttccatttcg tgtttttggc cgttccaacg ctgtaacctc
     7861 ttctgcggaa acaagttcaa aatctactgc tcgttcatct ggatcagctt tgactacttt
     7921 tactcgcact ttctgtccga ttttaaaagt cagaccagtt ctttcaccta caagagctaa
     7981 atgattttca acaaaatgga agtagtcctg tttcaattca ttgatatgga tcaatccctc
     8041 tactgtattc ggcagttcga taaacaggcc gaatttcaca acagagctga tgatcccatc
     8101 gaattcttcc cccactttgt ccatcatgaa ttctgctttc ttcatggaat ctacttcacg
     8161 ttctgtttct actgcacgac gttccatctt agaactatga tctgcaattt caggaagttc
     8221 cgtagcccac gaactttgat tgctttcact tttatcttca ctgtaagaac ggatcagacg
     8281 atggacgatt agatcgggat agcgtcgaat aggagatgta aagtgtgtgt agtaatctgc
     8341 cgctaagcca taatgcccaa aattgtcttc tgaatagcgt gcttgttgca tgcttcttag
     8401 caacatagta ttgataactg cagcttctgg tttgtcttct actccttcga tcacatgctg
     8461 taaatctttt ggtgtgatcg ttcctttcgt tcctcggacc agaatgccca aagctgaagc
     8521 aaaatcaaag aagcgctgca ttttttcttc ttttggctgt tcatggattc ggtaaataaa
     8581 cggcagattt aatttattat aatgttcagc taccgtttca ttggccgcaa gcatgaaaga
     8641 ttcgatcaag cgttcaccaa ttcctctgct tcttagctgg atatcatttg ggtgtccttc
     8701 agcatcaaca aggatttttg cttcgcgatc ctcaaaagaa atcgcgccac gtttgattcg
     8761 catcgtttca aggattcgat gcaattcacc catttcttgg aacataggta cgagctgagc
     8821 atagcgttgc atcgtttctt cgtcttgatc ttctaagatt tgattgacag ctgtgtaggt
     8881 catccgttct gttgtttgga tcacacttgg gaaaatatca tgagagacta cttgtccatt
     8941 tggatcaatc tccatttcgc aactcatggt cagccttgga acatggggat tcagtgaaca
     9001 gataccattc gataaacgtt gcgggatcat cggaatcact cgatcagtca aatatacact
     9061 tgtccctctt tcatatgctt ctctgtccaa ttgactgcct tgtgtcacat agtaagaaac
     9121 atctgcaatg tgtactccaa gaaaataatt accgttatct aattttttaa ccgtaactgc
     9181 atcatctaga tcttttgcat cttctccatc aatcgtaaca atgagctgat ttcttaaatc
     9241 tctacgtcca gttatatcct tttcgctgat ttgatctgga acttcatctg cttcagacaa
     9301 tacatcatca ggaaatttcg tagggatacc attagccacg acaattgaga ggatatccat
     9361 gccagggtca ttcttatgac caatcacttg cttgattatc ccttctaaac tagtcgcata
     9421 ccctttttca gggtaatggg tcagttcgat cagcacaata cttccgtcaa ctggacgaac
     9481 tccctctgct gctgcatata ctttaaattg gttgagcttt ctatctttcg ggataacaac
     9541 gccgtaaaga tccgtttctt gtatttcttc ttctgaataa gcaacgaatt cccctgctaa
     9601 ctgagtagtc gcccggtctc gaatctcgac tactttcccc tctgctccgc gatccatatc
     9661 tgtatttgct cgcttcatga tatcgattgc tacggtgtct ccatccattg caaaaccagt
     9721 cgcttctttt gggatataga tgtcatcttc ttcgggatca atcgttacaa atccaaatcc
     9781 tcgttcattt gctcgaaatg ttccttcgat caagattggt tttaaaggaa gtttcacttt
     9841 tcctttttta ttgaaaacta tactctgttc tctttccatt tgagcaatcg tttgtactag
     9901 taatttaaaa tcatcgcttt tttggaaccc taaaccttca gcgatttctt ccatagaaaa
     9961 actgcgtttg cgactgcttt ccatgaaaaa gagaattttc tcttttatcg tttcttttgt
    10021 catatttatt cctcattcca aggtaatttt tctaaaaatt ccaagacgtc tttttctaat
    10081 tcgcgtcttg ctgtaccaac tgtaatcaca tgaccacttt caggatacca tcgaagtgtc
    10141 actcgctgac ggctcaactt gcgtgcagtt tcaaatacgc cattagggtc gatcatttca
    10201 tctttcccag cttgagctaa aaatatcggc tgttggatat ctgacaacct actttctacg
    10261 attgctgcct gatcttgtat atccattaat tgttgttcag ctaacgagcg gtaagcaact
    10321 gctttttcat tgatttcttt ctctgattta cctgctgtct tcagcacttg acgtacgtat
    10381 ttttcaaagt tttcaggcac gtttgttttt acaggagaca aaggcgaaca gaaaaaaccg
    10441 ccgccaatca ctgattcttc ttctagtgct cgtacagcaa agatgccgcc cattgataat
    10501 cctaatacgg caatatcaga aaactgctca gactgtaaaa aatggatggc ttttttagtg
    10561 tctgcccacc agctttctgc tttttcttgc aaaatatcat atggctccat tgttccgtgt
    10621 cctttaaaaa gcggcgcata aactgtataa tctgcttttt ccaaaaatcg tgcgagcatt
    10681 cgaacatcgt ttggacttcc tgagtaagcg tgaagcagca aaaccgctcg ctttccatgt
    10741 tttgcataaa ggggttttgg taattgtctc actcgtctca cctctttcgc ctcattttac
    10801 catagtttct ttcataactt cttcttaagc gttctctttg caataaaaaa agccgcccgc
    10861 cgttttgcga gaggcttatt caactacttc gaagataaat atgctaggac aaataataag
    10921 atcatccaga tagctcccat aactgcagtt gatcgctgca tgacagcttc aaagccgcga
    10981 gctttttgtt tgccgaatag ttgatccgca ccgccggtaa atgcactcgc tgcactattt
    11041 tgcttgcttg gctgcatcat aatcgcaatg accataacaa tcgataaaac gatcacgatt
    11101 cccaaaatta aattatacat atcgcagcct ccttcccaaa agatatcgtc tctttaactt
    11161 tatcatagat aaaagtccgt gacaactcta tataaatgga ataacagctg aaagttcaaa
    11221 actttcagct gttattctta tgactgacgt aagacgtctt gatattctgg tttcttttca
    11281 aactcttttt ttgcataagg gcataaagga atgatctttt tcccgtctcg tcttgctaat
    11341 tctactccag tatacactag cttttcagct aacttttgac cacgatatgc tggatctaca
    11401 aatgtatggt caatgatcat gatgtctggg cccgcatctg accatgtcat ttctccgatc
    11461 tcttgttgtt catcattgaa taaagcaaga cgattttttt cttctttgat ttccatatgc
    11521 cgcactcctc aaataactga tattttttta ttcctacctt attttagcag tctcctgatc
    11581 tataaacaat tatttatcct cgaattactt acaaaaaaga agagaaaatt aattcatcgt
    11641 tattctttgt tcaataaatg aaaaaatcac tgtccaatag gttttcggat ctttcatcga
    11701 tgcttccaag tgttctgctt ctggaacaag cagcagtgct ttatcccctg ccgtagcatt
    11761 ttgaatcgtg tacgctgctt ctgtagggac aaaatgatct ctttcaccat gaatgaccaa
    11821 taaaggcaga tggttgctgc caagctgtct cgtagctgat gcttgcttta aggagtaacc
    11881 ggcgtatttc tgggcataat gattcgcata tctcatgacc atttttttag gaaagacagt
    11941 gattttactt aacatatatc ggaattctgc ataaacagaa acatatccgc tgtctaaaat
    12001 aaatcctttg accgctgatg gaagtttttc accacttgcc atcatgatcg tagaagctcc
    12061 cattgatccg ccgtgtaaaa taatggacgc attcggctgt tcatctaaga tgagttggat
    12121 ccaggcaatt aaatctagcc gatctagcca tcccattccg atgatttctc cttcactttc
    12181 tccgtgcgca cgtaaatcag gtacaagaac gttgaatcct ttctctgcat atcttttacc
    12241 tatatgagac atgtctcttt tccctgtgct tcggtaatca tgtacgcaaa tgacccattt
    12301 attctgctta tttggatgga tgaacatctg accagctagt ttcaacccgt cataactttt
    12361 gattgttttg ggtgttccct gctcccaaaa gctttcacac tcttcttcta atttttgtcg
    12421 ttttttttct actgacgtga ctgcagcagg gtccatcaaa cgatgaccag tgtcttcata
    12481 ccagtcatat cctctctgta ctcccgctct tactaatttc tttgcataaa aataagaaag
    12541 aaaatggtaa ctgatcccta taattatcag aataagaatg aaataaaaaa taaccattgt
    12601 acacccgccg ctttctgcca aactataatc taactattat ccaatgtacc gatccatagg
    12661 attctttatt ttcggcacac gtaaattcca tttctttttc attgtatcat ttacttaaag
    12721 ttctcctcaa tagatttact tgtaagagag catttacatt cgcaataaaa gccagtatac
    12781 taatttcgtg cattataatc aagacgctcg tcttggactg gttcggaaac ttcccagaat
    12841 aaaaaactaa gtatctctaa agaaaggaga ttggaaataa tgcgaaaagt aattattgat
    12901 tgcgatccag ggatcgacga cactttagct ttatcattag ctgtcaaatc ccctgatatc
    12961 gaagtagttg ctattaccgt agtttgtggg aatgtccctg cagatatagg tacgcagaat
    13021 gtgctgaaat gcctggaacg ctgtgatcgt ttagacattc ctgtttatca aggaatggaa
    13081 aaacctttga agcaaccttt catcagtgca caagacactc atggtatgga tggtttaggg
    13141 gaaacaaatt tcccgatgat cttgagaaaa caggcagaac ctctccatgc cgttgatttt
    13201 ttggctgatt atttcaaaga aaagacaaat acatcagtca ttgctttggg tcctttgacc
    13261 aatatcgctt ttgctttgaa agtcaaccca aatattggga aacatatgga tcgttttgtc
    13321 tcgatgggtg gaacttacaa aagccatggt aactgttcgc cagttgctga atacaattac
    13381 tggtgtgatc ctgaagcagc ttcttatgta ttcgagaatc tcaaacaaac gatagaaatg
    13441 gttgggttag atgtgacaag agaaatcgtc ttgacaccga ctatcctcga atactgctgt
    13501 caaatgaatc ctgaagaggg agagtatcta aaagcgatca ctcgttttta ttttgacttc
    13561 cactggaaac aagaaagaat tttaggatgt gtcatcaatg atccgttagc tgttgcttat
    13621 tttatcgagg gggccatctg tgaaggtttc aaaagcttca ctgctgtgga aactcaagga
    13681 ataagtcgtg gacaaacatt ggttgatcgt tatgaatttt ggcagaaacc tgctaacagt
    13741 aaaattatga caaaagtaga tactcgtctt tttttccgaa agtttttaac cgttctttta
    13801 aatgcacaag aagaaacgat tatgaaagat ttggaaaggt taaagatggg ctaacaaaat
    13861 gaataaaaac aaaatggcaa ctcgcgtact tacagtgatg gctttgtgta ttgggatcaa
    13921 ttatattgga ggaacgattg ctttatggct tcgtttgcca atctatctcg attctatcgg
    13981 aacgattttt gctggtgcat tgcttgggcc tgtacctgga atgctgacag gattgtccag
    14041 cggtatttta agtggagtga ctacagatat tttttctctg tattattcgc caatccaaat
    14101 tctcacaggc ttgcttgctg gattgatttt gccaagatat ctcggcaaag ataaaaacag
    14161 cttgaaactg ttattactcg ctttgatttt gtcggtacct ggaacgatcg tttcttcgat
    14221 cattaccgtt cagttatttg gcgggatcac ctcctcagga tccagtatga tcgttcagct
    14281 attacatggg ttaggtttaa gtcagacaac aagtgtgatc ttggtacagg caggtacaga
    14341 ctaccttgat cgtttgttgt ctatcgtcgt tgtttcatta gtcatcacta tgttgccaca
    14401 acgcttgata tattcaaaaa gaaaaatagg ctgaataaac agagctggat tagcaaaata
    14461 atgctaatct ggctctgttt tttcatttat taaaaaaatc ttacgctaag taggataaaa
    14521 taattccttg atatttttca atcatatcca aataattatc gatttcaaca tactcatcta
    14581 cttgatgagg caaagtaacg acaccaggcc caaagacgac aaagtcgaag gaatggttag
    14641 ctttcgtaaa ttcggctgca tccgttgttg ctgccgctcc aactaaaggt aatggctggc
    14701 tgaattgttg ctgaatgcaa tgaatcaaag gagaatctgg atcagctttg acaggtattt
    14761 tgttgtaatc gatcatcaat tccaaatgat agtctgtctc ttgattcaat tcattgacga
    14821 ttgattgcag caatgcaatg attttatcat ttgggtattc tggaatcgat cgaatgttcc
    14881 cttggagctg cgcatggctt gggatgctgt tgacttgatt cccgccactg atcaatgtaa
    14941 cattatggat tgtttttcct aggacaggat tctcgattgt ttcagcgagg tgattcattt
    15001 cagcatttgc tttcgtaatg aattcattta ggtgattgat tgcattataa ccttggtctg
    15061 gcatagagct atgcgcttct tttccgtgcg aggtgactgt atagttgatt gatcccatgt
    15121 gcgtatacat caaactgtaa ttagttggct ctccgatgat caaggcatct aagtcatcta
    15181 catagcctgc tttcgtgagc tgttccccac ctagttcacc cacttcttct ccgacagtgg
    15241 ctaataattt gactgttccg ttaaatggtt ttcctgactc tttcagttcg atcatggcaa
    15301 tcaccattgc tgccaagcca ctcttcatat ctgtagcacc acgtccgtac aaacggttcc
    15361 catggatttc tgctgcaaag ggtgcatagg tccaagacga ttcatcacca gccgccacga
    15421 catccatatg cccagatagg cctaacactt ttcctgattg gcccttttgg taacgagcaa
    15481 tcaaattatc tctaccatct ctgtaagaaa caatctctcc tgttatgtca tgctttgcca
    15541 aaagcttatt caaataggct gctacttcac cttcgtttcc attcactgat ttaattcgga
    15601 tgatctcttg taaaatcgcg attttttctt cttttttcat ctaatcctct cccttgccat
    15661 ttctttttat tgtatttgag tgtgctcgaa ttttcaaata ggagcaacca aagtaaaaat
    15721 gaagaaagag agccacttta tgaaaaatat gctgctactt ccctcctatt ttatttatac
    15781 gagaggactt gctcatagat tcctattcta ctgttctatc cttaaaagct cttatatgat
    15841 gctagttatt gttagctttc ctttttcaag gatgatcttt tcttgtacca ttctttgtaa
    15901 acatcatata cagaagctag ttgcaaaaaa aactctctac ctgctgat
//
LOCUS       DACDKM010000065        15846 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20834_2016
            SAMN12640370-rid9891663.denovo.065, whole genome shotgun sequence.
ACCESSION   DACDKM010000065 DACDKM010000000
VERSION     DACDKM010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640370
            Sequence Read Archive: SRR10040891
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15846)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15846)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742835.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742835.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:04:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,012
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 29 of 75
            Pseudo Genes (incomplete)         :: 33 of 75
            Pseudo Genes (internal stop)      :: 19 of 75
            Pseudo Genes (multiple problems)  :: 5 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15846
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640370-rid9891663.denovo.065"
                     /isolate="E20834_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            130..1557
                     /locus_tag="IV334_001829"
     CDS             130..1557
                     /locus_tag="IV334_001829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HAP8943893.1"
                     /translation="MIEEYIEKNILRQLFLCGQFYVNKEVNLEKLSNLLHVCKTTLLN
                     DINNIKKEFEEQIAYTHREKDRYTLYFSEHIPRCKIMQQLSQNSLFLKTCLLYLEEDE
                     PDYLQLTECEFISVSKAYSLKKQVLAYFNDCGIEIDRYSPRFTEMERRLLLLNVSYRL
                     GGFNSWELPESFFERADRFIESVTENSGRFYDKENKEILSIGFAISFLRQQVCAVTID
                     SKFIEEIKKRPIYNYVESAWENTDFQTYYKKEEFAFILTLFNLCNYGFHSYQLIAEDF
                     QQLHQVFIDNTPEIKELVATFESHFNQELFGNQPFERALIHLMRSAWDNYQLFMPEKF
                     YLLNEEQTNLYKEVQTIFSSWSSQLPYDLRLNPNCMRAFVIELSGILRLTKEHLTIYI
                     VTNSDVHYLIYREALEAVTTFDFQVAPTIYSSISDIKKYAQQSSNRVLCERTLYTPDA
                     VQYENIIPISINTIDRAIISAVQNK"
     gene            complement(1649..2014)
                     /locus_tag="IV334_001830"
     CDS             complement(1649..2014)
                     /locus_tag="IV334_001830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1622 domain-containing protein"
                     /protein_id="HAP8943894.1"
                     /translation="MTTELMQFLLPYFEKLVLGLDIFAICLLVWGVCLSIKDFLKYLF
                     VHTKVQSDRVSENNAIKKMLGGYILLSLEILVSAGIIESIIKPTLQDIFQLAALVVIR
                     TIISYFLNKEIGPLDVQNK"
     gene            complement(2011..2376)
                     /locus_tag="IV334_001831"
     CDS             complement(2011..2376)
                     /locus_tag="IV334_001831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1622 domain-containing protein"
                     /protein_id="HAP8943895.1"
                     /translation="MFRSNLIEFLEWIIAILNICSIGILIVGIVLVLFKLFDWSHVKK
                     NYAERNTRNADTRKLLASYILLSLEVLVVADIIESVIKPTWTDILKLALIIIIRTVIS
                     YFLNQEIEDKEKQRRGEVG"
     gene            complement(2568..2816)
                     /locus_tag="IV334_001832"
     CDS             complement(2568..2816)
                     /locus_tag="IV334_001832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8943896.1"
                     /translation="MKNASLFSLSISKDSSFLLECQMIGREEKGNFDKKEEVSDFPVN
                     KNRVGKKKRFEEVYGSIFRNLGTKKAPRQCFFEKLMTD"
     gene            2815..2943
                     /locus_tag="IV334_001833"
     CDS             2815..2943
                     /locus_tag="IV334_001833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methanol dehydrogenase"
                     /protein_id="HAP8943897.1"
                     /translation="MSKKLIKVGIGLGLLALGAAYLGKKTGLFEDDSHLYDEFESI"
     gene            3000..4694
                     /locus_tag="IV334_001834"
     CDS             3000..4694
                     /locus_tag="IV334_001834"
                     /EC_number="4.2.1.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oleate hydratase"
                     /protein_id="HAP8943898.1"
                     /translation="MRKSKAIMIGAGIANMAAAVYLIQEGKWKGDQITFYSLDDHGSN
                     DGAPTDTVVDEYWNKNHPLENQKGYIARGGRMLNYRTYVDLMDLLSRIPSVTEPGMTA
                     EEDTRDFDAKHRTFDKARLMEGGKGIIDGGKLGFNNKDRILLTKLIAMPDSEEEKLDN
                     ITIEEYFKDDPHFFETNFWFMWETTFAFRTRSSAQELRRYMHQMIYEFTQIEHLVGVN
                     RTRYNQYESIMLPLIEYLKEQGCKIILNRRVTDWEFKETPMQDEITVTGLKITNTLTQ
                     EEEYVVVDEDTAVIFTNGSITDSATLGDYETPAVENMDYGAASSLWKKASERFYNLGN
                     PDKFFADRDASEWVSFTLTTKNHLLLNEITRITTQEPGNALNSFISTTPITPLGQKDV
                     NMSIVVHHQPHFTSQKPNETVIWGYFLYPRRRGEFVDKQYIKMNGKEMLEELIGQLSK
                     VDPGPVNIREKETEIFDSVINNIPVYMPYASALFNNRAKSDRPKVIPKHSTNLAFTGE
                     FVEQPYQMIFTEQSAVRSGEIAAFHFAGIPMSRLVKTPRYDKDIPTLARAAKKMFE"
     gene            4794..4967
                     /locus_tag="IV334_001835"
     CDS             4794..4967
                     /locus_tag="IV334_001835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8943899.1"
                     /translation="MQAFEHRLPRGYEKAALHQVIRAAKLKQLLMFLTWLDLLPQMQA
                     FEHRLPRGYEKGV"
     gene            5345..6478
                     /locus_tag="IV334_001836"
     CDS             5345..6478
                     /locus_tag="IV334_001836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HAP8943900.1"
                     /translation="MKAVTWQGKQKMEIRNVDDPKILEPTDAIIRITATAICGSDLHL
                     YHHGEAVLEKGYVVGHEPMGIVEEVGSQVQTLKKGQRVVIPFNISCGHCHFCLNHMES
                     QCDESNPDQLYGGLFGFGKLNGNHWGGQAEYLRVPYADSTSFVVPDNDLPDEKVLFLS
                     DILPTAYWSVENAGVKKGDTVVILGSGPVGLFAQKFAVMAGAERVIAVDPVQHRLDKA
                     ANYNGVETYFLEDTAQAGTELYELAKGGADVIIDCVGMDGLEPVKEKAKNLVSLQSGT
                     ISPLQMASQAVKKFGTVQVTGVYMTPASSYPLQEFFMRDIAVKHGQAPIIHLMPKIYA
                     MIAEGLFDPTQIITHSLPLSEAAQAYEIFDKKEDKNIKVVLKP"
     gene            6509..6829
                     /locus_tag="IV334_001837"
     CDS             6509..6829
                     /locus_tag="IV334_001837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8943901.1"
                     /translation="MPKIKRNTHEIEIGKEEDIEIRGRRFRFYFQNRYTLISLAVDLL
                     TGIFYIFGSLASLTKIPDRYGTYFYLAGAIFLTIRPILRIVRNIFIYDEKKAMKRTSK
                     KKEA"
     gene            complement(6858..7322)
                     /locus_tag="IV334_001838"
     CDS             complement(6858..7322)
                     /locus_tag="IV334_001838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP8943902.1"
                     /translation="MVTYNWIKLREHPELVKQSANWFSSKWGVPAVVYEESIRTGIAN
                     KESIPQWYVVLDEKETIVGGAGVIENDFHNRPDLTPNLCALFVEPEKRKQGIAKRILA
                     EIRKEMHQLGYEKLYLITDHTTFYERYEWTFLTMVKEESGTTARLYQSDTIG"
     gene            complement(7454..8707)
                     /locus_tag="IV334_001839"
     CDS             complement(7454..8707)
                     /locus_tag="IV334_001839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAP8943903.1"
                     /translation="MDKFVAFMEKHFIPVASKIGAQRHLVAIRDSFMVSMPLMILGAL
                     AVMINNLPIPGFQELMNSIFGGESWKGFGAAAWNGTFAILSVLIAFLLAYHLANGYRK
                     DGVSAGVISLGSFFALGGALGMSSNGLFIAIIVGIISTEIFVRLSGNEKLIIKMPDGV
                     PPAVAKAFASLLPAMITISAFALVAAIFAAFGVDDIVGSFYTVVQEPFMGLANSYPSA
                     LLLAFITPFLWFFGLHGANMVDPLMQTINAPAIEANVNAIAAGHSAPFIVNKPFFDSF
                     VNLGGTGATLGLLLAIYLVGRKNKPYMVVTNLSIAPGVFNINEPTMFGLPIVLNPIMF
                     IPFILTPMVLVSVAYFATSTGLVPAATVMPPWVTPPIIGGVLATKSIAGGVLAAVNLV
                     LSILIYLPFVKVATIQERRKEALDA"
     gene            complement(8736..9053)
                     /locus_tag="IV334_001840"
     CDS             complement(8736..9053)
                     /locus_tag="IV334_001840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HAP8943904.1"
                     /translation="MEDQKNLEAIMGLIMFGGNAKSDAMEAIAAAKKGDFTLADEKIK
                     DAEESLVQAHHSQTEMLTQEAQGNHVEVTLLTVHSQDHLMTSIAFTDLAKEIINLYRR
                     LNA"
     gene            complement(9079..9399)
                     /locus_tag="IV334_001841"
     CDS             complement(9079..9399)
                     /locus_tag="IV334_001841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAP8943905.1"
                     /translation="MEKKTIMLVCSAGMSTSLLVTKMQKAAEAKGMEADIFAVSASDA
                     DNQLESKNVDVLLLGPQVRFMQNQFAEKLAPKGIPLDVINMADYGMMNGEKVLDQAEK
                     LINK"
     gene            complement(9863..11278)
                     /locus_tag="IV334_001842"
     CDS             complement(9863..11278)
                     /locus_tag="IV334_001842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HAP8943906.1"
                     /translation="MKITIPKNFILGAASSAWQTEGWKGKKEGQDSFIDSWYKNEHFV
                     WHNGYGPAVATNFMEQYQQDVDLMKSIHLTHYRTSINWARFFTDYENCIVDEEYAQHI
                     SEMIDSLIEADVEPMICLEHYEVPAFLLEKYDGWSSRKVVDFYAKYAQIAFDRYGDRV
                     KNWFTFNEPIVPQTRCYLDAVRWPHEQNTKKWMMWNYHKVLATAKAVEIYRSGNYGGR
                     IGCILNPEMVYARSNSPEDQKAAYMYDLFYNRVFFDPMVKGYYTEEFLELCQKQSIYF
                     DPATEDLETIQHHTVDFLGINQYYPKRVRAPRYQWNEETPFHPERYYEVFDLPGKKMN
                     NSRGWEIYPKIMYDIAMYLKENYHNIPWLITENGMGREYEEQYMDEKGIVQDDYRIEF
                     IGQHLHYLIKATEEGANCEGYMLWAFTDCVSPMNAFKNRYGLVRIELNDERSRTLKKS
                     ASWYQTLIDKRELDLPDEIYK"
     gene            complement(11295..12611)
                     /locus_tag="IV334_001843"
     CDS             complement(11295..12611)
                     /locus_tag="IV334_001843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAP8943907.1"
                     /translation="MRGSGMSFMDKLANVLGNVATRINNLRYIMVIKNAFAALIPVII
                     TGAFGTLFSAMVFDSENGLAQISFLRFLEELKPIASAISYVTLSFLTIYAVFLIGIEL
                     AKLNKVDGVFPGIIAVMSYLSVNPTVYELVNENQTVIVENVLAKQYTDTKGLFLGMLV
                     SILSIELYCWLRKQKKLQLRMPDTVPTNVSASFSALFPTILTVSGIAAAGFIIKELTG
                     MYLYDIIYNVVQRPLEGVVQGLPGILLLMFIAQVFWVIGIHGNQMVKPIREPLLLAAI
                     AVNTEAFEAGKEVPNIITMPFWDMYMSMGGSGVTIGLLIAVLLVGKREDMKQITKLSL
                     APGIFNINEPVIFGMPIMLNPILAIPFILTPLITGTIGYFATALGFAAKAVVMVPWPM
                     PPIVNAYLATAGNIGAVITQLVCIIVSILIYLPFVKVANRVENDGI"
     gene            complement(12761..13459)
                     /locus_tag="IV334_001844"
     CDS             complement(12761..13459)
                     /locus_tag="IV334_001844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FadR family transcriptional regulator"
                     /protein_id="HAP8943908.1"
                     /translation="MEENKTLVEVTTDKILRYLKEMQLQVGDKLPNEYELSDYLEVGR
                     STVREAVRALASRNILEVRQGAGTFVSDKRGIANDPLGFSLIEDQDRMIRDLFELRYL
                     LEPKMAGLAAEYATKNQIVQMKKLTQKIETSFREGTQEHVQLDIDFHTMIAEASGNVA
                     LLHIIPIINESIALFNKNYDIQHLKIETIDIHTEIVQAIERRNALAAFDAMTVHMAIN
                     RRELQKIIGEKETN"
     gene            complement(13748..14737)
                     /locus_tag="IV334_001845"
     CDS             complement(13748..14737)
                     /locus_tag="IV334_001845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-glyceraldehyde 3-phosphate reductase"
                     /protein_id="HAP8943909.1"
                     /translation="MYIADSARYEKMEYRKSGHSGLKLPILSLGLWQNFGDYDPIHNQ
                     REILRGAFDMGITHFDLANNYGGPAGAAEKNFGRIFREDFQAYRDELIISSKAGYHMW
                     EGPYGEWGSRKSIISSCDQSLQRMGLDYVDIFYHHRPDPDTPLEETAEALMQLVRQGK
                     ALYIGISNYNGEDTKKMTEILKRKEAPFIIHQMRYNMFSRALLEDDLSPVLEEEGLGA
                     ITFSPLAQGLLTNRYLHGIPEDSRAHRKEIPFLSEEQVGSTLEKIKALQTIAVSRGQS
                     LAQMALAWNLRQKSVTSVLVGASRLSQLQESVRMMDNLDFSPEEELRIDQILE"
     gene            complement(14817..15650)
                     /gene="msrA"
                     /locus_tag="IV334_001846"
     CDS             complement(14817..15650)
                     /gene="msrA"
                     /locus_tag="IV334_001846"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HAP8943910.1"
                     /translation="MDEEQIFIQTLYNLILNPNTRDWERKVLIQTKNDIRENISVKEQ
                     LSKLEATLRPLAIRMNLTPDVMDFYLLITEGFDKEQKYDFSKHAMQDADYQERAVFAG
                     GCFWCMVEPFESKKGILSVLSGYTGGHVEKPNYDQVSGGYTGHVEAVEIIYDTREISY
                     SELLTIYWQITDPTDTFGQFQDRGKQYRPVIFYQVERQKELAEQSKQKLDSSGTFHQP
                     IVTKIEPAGTFWPAENYHQQFYKKQPKRYKKIQQARNQFLIYQRVKNKWQKNIRKNHF
                     D"
BASE COUNT         4937 a         3202 c         2702 g         5005 t
ORIGIN      
        1 ttataacata aaaaaccttt tttaacggca tttatatgct ttttcagaaa ttaactgatg
       61 atttcaaaaa gaaaacatgt cataatattt cccatattgt gcaaaaaaat acaaggcgga
      121 ggctctacta tgattgaaga atatattgag aaaaatattt taagacagct gtttttatgt
      181 gggcaattct atgtaaataa agaagtcaat ctagaaaagc tctccaatct cctgcatgtt
      241 tgcaaaacca ctttactcaa tgatatcaat aacataaaaa aagaatttga agaacagatc
      301 gcttatacac atcgggaaaa ggaccgttac actttatatt tttcagaaca tatacctcga
      361 tgcaagatca tgcagcaatt gtctcaaaat tcgttgtttt taaaaacttg tctgctttat
      421 ttggaagaag atgaacctga ttatcttcaa ttaactgaat gtgaattcat ctctgtcagc
      481 aaagcttatt cattaaaaaa acaagtatta gcttacttta atgactgcgg gatcgaaatc
      541 gaccgctact cccctcgatt cactgaaatg gaaagacgtc tgctgcttct taatgtgtcg
      601 tatcgtttag gcggttttaa ttcttgggag ctacctgaat cgttttttga acgtgccgat
      661 cgtttcattg aatccgtaac ggaaaatagt ggcagatttt atgataaaga aaataaagaa
      721 atcttatcga ttggttttgc tatcagtttc ctacgccagc aagtgtgtgc tgtgacaata
      781 gattcaaaat ttatcgaaga aatcaaaaaa cgtcctattt acaattatgt cgaatcagca
      841 tgggaaaaca ctgatttcca aacctattat aaaaaagaag aattcgcctt tattttgaca
      901 ctattcaatc tttgtaatta tggtttccat tcttatcagt tgattgcgga agactttcaa
      961 cagctacacc aagtgtttat tgacaatact cccgaaataa aagaactagt tgctaccttt
     1021 gaatctcact tcaatcaaga attattcgga aaccaaccgt tcgaacgtgc gctgattcat
     1081 ctgatgcgtt cggcttggga taactatcaa ttattcatgc cagaaaaatt ttatctttta
     1141 aatgaggaac agacaaattt atataaagaa gtccagacaa ttttttccag ctggtccagc
     1201 cagttacctt atgatctacg gttgaatcct aattgcatgc gggcttttgt gatcgaattg
     1261 agcggaattc tccgtctgac aaaagaacat ctgactatct atatcgtcac aaattcggat
     1321 gttcattatc tgatttatcg ggaagccttg gaagctgtaa ctactttcga ttttcaagtt
     1381 gcaccaacaa tttattcaag tatatctgat ataaagaagt atgcgcagca gtcctcaaac
     1441 cgggtattat gtgaacgtac attatataca ccagatgcgg tccaatatga aaatattatt
     1501 cctatttcga tcaatacgat cgatcgagct atcatttcag ctgtgcaaaa caaataaggg
     1561 gctgtgacaa aaatcatgtc acagcctctt atttgttgaa gatgcccatt tctgtaacaa
     1621 tctcttgata cacgtcaggc tcacaacctc acttattttg gacatctagg ggtccgatct
     1681 ctttatttaa aaaataagaa atgatggttc ggataactac caacgccgct agttggaaaa
     1741 tatcctgcaa ggtaggtttg ataatcgatt cgatgatccc agctgaaaca agaatttcca
     1801 aacttagaag tatatagcct cccaacatct ttttaatagc attgttctcg cttactcggt
     1861 cgctttgcac ttttgtatgg acaaataaat atttcaaaaa atccttgatt gatagacaaa
     1921 caccccaaac caataaacag atagcaaaga tatcaagtcc cagtacaagt ttttcgaaat
     1981 atggaagaag aaactgcatc aactctgtcg tcatcctacc tctcctcttc tttgtttttc
     2041 tttgtcttct atttcctgat tcaagaaata agaaatgact gttcgaatga taataataag
     2101 tgcaagcttc aaaatatctg tccaagtcgg cttgataact gactcgatga tatcagcaac
     2161 gactagtact tccaagctta acaaaatata acttgctagc agttttcttg tatctgcatt
     2221 acgagtatta cgttctgcat agtttttctt gacatgagac caatcaaaaa gtttgaatag
     2281 aacaagaacg atcccgacaa ttaagatacc gatagaacaa atatttaaaa tagcaatgat
     2341 ccattctaga aattcaatta aatttgagcg aaacatatac gctacctact ttcttttaat
     2401 ttgttcctat tcttttattg taaaagaaag ttaccttgtt cgtctattta aaagttgttc
     2461 taatgataac ggttcgctta aatttttctt tttcactctc ccatttttct ccctccatcc
     2521 ataaatcgtg gaagtatcca aaacaacgta gcaagttaaa ataaggctta gtccgtcatc
     2581 agcttttcaa agaagcactg tctcggtgct ttttttgtac ccaaatttct aaaaatagaa
     2641 ccatacacct cctcaaatct tttcttctta cctaccctat ttttatttac aggaaaatca
     2701 gacacttctt cttttttgtc aaaatttcct ttttcttctc gtcctatcat ttggcattct
     2761 aataaaaacg aactatcctt ggatatagaa agagaaaaca aggaggcgtt tttcatgagt
     2821 aaaaaattaa tcaaagtcgg catcggttta ggtttgcttg cccttggcgc tgcatatcta
     2881 ggaaaaaaga caggactatt cgaagatgac agtcatttat atgatgaatt cgaatcaatc
     2941 tagcatccta tttagttgaa tcactacgga catacttgga agacattgga ggaatcacaa
     3001 tgagaaaaag taaagcaata atgattggtg ccggtattgc caatatggca gcggctgttt
     3061 atttgattca agaaggcaaa tggaaagggg atcagatcac cttttattca ttggatgatc
     3121 acggttcaaa tgatggtgca ccgaccgaca ctgtagtgga tgaatattgg aataaaaacc
     3181 atccattaga aaaccaaaaa ggctatattg ctcgaggcgg tcgcatgctt aattaccgta
     3241 cctatgtaga tttgatggat ctgttaagtc gaataccttc tgttactgag ccagggatga
     3301 ctgctgaaga agatacgaga gattttgatg caaaacaccg aacctttgac aaagcacgtt
     3361 tgatggaagg tggcaaagga atcattgatg gcggaaaact gggattcaat aataaagacc
     3421 gtattcttct gactaaactg attgctatgc ctgattcaga agaagaaaaa ctagacaata
     3481 tcactatcga agaatatttc aaagatgatc cacacttttt tgaaacaaac ttctggttta
     3541 tgtgggaaac aacatttgct ttcagaacac gtagttctgc tcaagaattg cgtcgctata
     3601 tgcatcaaat gatttatgaa ttcactcaaa tcgaacatct agtgggcgtc aatcgaacac
     3661 gttacaatca atacgaaagt attatgcttc ctttgatcga gtacttgaaa gaacaaggat
     3721 gcaaaatcat tttgaaccgc cgtgtcactg actgggaatt caaagaaaca ccgatgcaag
     3781 atgaaatcac tgtgactggt ttgaaaatta ccaatacact gactcaagaa gaagaatacg
     3841 ttgtggtcga tgaagataca gcagttattt tcactaatgg gtccattaca gattcagcta
     3901 ctttaggaga ttacgaaaca ccagctgtag aaaatatgga ttatggtgca gcttctagtc
     3961 tctggaaaaa agccagtgaa cgtttctaca acttgggaaa tcctgacaaa ttctttgctg
     4021 atcgagatgc aagcgaatgg gtcagcttta ccctgacgac gaaaaaccat ttgttgttga
     4081 atgaaatcac ccgtatcacg actcaagaac caggaaatgc cttgaattca tttatttcaa
     4141 ccacaccgat cacgccgtta ggccaaaaag atgtcaatat gtctattgtc gtgcatcatc
     4201 aaccgcattt tacgtctcag aaaccaaatg aaacagttat ttggggttat ttcctttatc
     4261 caagacgacg tggcgaattt gtggataaac agtatattaa aatgaatgga aaagaaatgc
     4321 tagaagaact gattggacaa ctttcaaaag tagatcctgg accagtgaat attcgtgaaa
     4381 aagaaacaga aattttcgac agcgtaataa ataatatccc tgtctatatg ccttatgctt
     4441 ccgcgttgtt caataatcgt gccaaaagcg atcgaccaaa agtcattcct aaacattcga
     4501 ccaatctggc ctttacagga gaatttgtcg agcagcctta tcagatgatc tttactgaac
     4561 aaagtgctgt acgttctgga gaaatcgctg ctttccattt tgcgggtatt cctatgtccc
     4621 gtcttgtcaa aacaccgcgt tacgacaaag atattcctac tctagctcgt gccgcaaaaa
     4681 aaatgtttga ataatcaaga ggttgtgaaa aagctgccct gcatcaagta ataagaacaa
     4741 ccaggctaaa acagcttctt atgtttttga tttggttgga cttattaccg cagatgcaag
     4801 cttttgaaca ccgtttacca agaggttatg aaaaagctgc cctgcatcaa gtaataagag
     4861 cagccaagct aaaacagctt cttatgtttt tgacttggtt agacttatta ccgcagatgc
     4921 aagcttttga acaccgttta ccaagaggtt atgaaaaagg cgtttagttt cgagtatgaa
     4981 gaaaaatttt gtgaaaatgg ttcctcacgt ttttgcataa atttgactta ataccgagaa
     5041 actgatccct tctgtcataa cctcttttta tttatgtctg tttctttttg aaaacaacta
     5101 ttttttcaac gtttactctc ttgccaatcc aacatcatct aacggataca actatttcaa
     5161 tgattaccat tacactaaaa aatcggaaac acacctctcc gaacacaaat acataagact
     5221 atagaatgtt tcatttaaga aaaaaagcgt ttcattatca aaaaggtaag atctccttta
     5281 aaatgaaatt atctgtagta ctaataatgc agacaatttt atgaatctga aaggagcatc
     5341 tccaatgaaa gcagtcactt ggcaaggaaa acagaaaatg gaaatcagaa acgtagatga
     5401 tccaaaaata ttagaaccaa cagatgcaat catccgtatc acagcaacag ccatttgcgg
     5461 ctccgatctg catctttacc atcatggtga agcagttttg gaaaaagggt atgttgtagg
     5521 acacgaacca atgggaatcg ttgaagaagt gggatctcaa gttcaaacat tgaaaaaagg
     5581 gcaacgtgtg gttatccctt ttaatattag ttgtggtcat tgtcatttct gcctgaatca
     5641 catggaaagc caatgtgatg aatcaaatcc agatcagtta tatggcgggc ttttcggatt
     5701 tggcaagttg aacggtaatc attggggtgg tcaagcggaa tatctaagag ttccttatgc
     5761 agactctact tcatttgttg taccagataa tgatcttcct gatgaaaaag ttttatttct
     5821 atcagatatt ttgcccacag cttattggag cgttgaaaac gcaggagtaa aaaaaggcga
     5881 tacagttgtg attctaggtt ctggtccagt cggtctattc gctcaaaaat tcgctgttat
     5941 ggctggagca gagcgcgtaa tcgcagtaga tccagtacag caccgtctag ataaagcggc
     6001 taattacaat ggggttgaaa cttatttctt agaagacacg gcacaagctg gaacagaatt
     6061 gtatgaactg gctaaaggtg gcgcagatgt cattatcgat tgtgtaggaa tggacggatt
     6121 agagcctgtc aaagaaaaag cgaaaaattt agtaagctta caaagcggca caatctctcc
     6181 attacagatg gcatcgcaag ctgtcaaaaa atttggaaca gtccaagtaa ctggtgttta
     6241 tatgactcca gcttcttctt acccgctgca agaatttttt atgcgggata tcgctgtcaa
     6301 gcatggacag gcaccaatta tccatctaat gccaaaaatt tacgcaatga ttgcagaagg
     6361 actattcgat cctacacaaa tcattactca ttcgctgcca ctatcagaag cagcacaagc
     6421 atatgagatc tttgacaaaa aagaagataa aaatataaaa gttgttttga aaccttgaat
     6481 tcagtaaaaa atacaggtga ttttctttat gcccaaaatc aaacgaaata cccatgagat
     6541 cgaaatagga aaagaagaag atatcgaaat acgaggacgt cgcttccgct tctatttcca
     6601 aaatcgttac acattgatct cactggctgt cgatttatta accgggattt tctatatttt
     6661 tggaagcctt gcttccttaa ctaaaatccc cgatcgatat ggcacctatt tttatttggc
     6721 tggtgctatt ttcttgacga ttcgtcctat tctcagaatc gtaaggaaca tttttattta
     6781 cgacgaaaaa aaagcaatga agagaacaag caaaaaaaaa gaggcatgag aatttttcaa
     6841 atctcatgcc tcttttttca ccctatcgta tcactttgat acagacgagc ggtcgtccca
     6901 ctttcttcct tgaccattgt caaaaatgtc cactcgtatc gttcataaaa cgtggtatga
     6961 tctgtaatta agtaaagttt ttcgtaccca agttgatgca tttcttttct gatttccgct
     7021 aatatccgct tggctatccc ctgcttacgc ttttccggtt caacaaacag ggcacaaaga
     7081 tttggtgtca gatcagggcg gttatggaag tcgttttcaa tgacgcccgc tcctccgacg
     7141 atggtttctt tttcatctaa gaccacatac cattgaggaa tactttcctt attagcgatt
     7201 ccagtacgaa tactttcctc atacacaaca gcaggaactc cccattttga tgaaaaccaa
     7261 ttcgcgcttt gttttactaa ttctgggtgt tctcttaatt tgatccagtt atatgtaacc
     7321 aatgtttaaa cctccgacta aaataaaacg tttacaaaaa ttatacaagt ctttatgaaa
     7381 attagcaatg acaacaatag tgatgtaaaa aaaaaaacgg aaaacaatca cttgttctcc
     7441 gttttttgat taatcaagca tctaaagctt cttttcttct ttcttggatc gtagctacct
     7501 tgacgaacgg tagatagatc aaaatagaaa gaaccaaatt gacagcggct agtacaccac
     7561 ctgcaatact ttttgttgcc aatacaccgc caataattgg tggagttacc catggaggca
     7621 taacggtagc tgctggcact aaacctgtag aagtcgcaaa gtaagcaaca ctaactaaga
     7681 ccattggtgt caaaataaat ggaatgaaca tgatcgggtt caacacgata ggtaacccaa
     7741 acatcgttgg ttcattaata ttgaagacac ctggcgcaat cgacaagttt gttacaacca
     7801 tatatggttt gtttttacgt cctactaaat aaatagctaa cagtaaacct aaagttgcac
     7861 ctgttcctcc taagttgaca aagctgtcaa agaaaggttt gttcacgata aacggtgcgc
     7921 tatgtccagc tgcgattgca ttaacgtttg cttcaatggc tggcgcattg attgtttgca
     7981 tcaaaggatc gaccatattc gctccgtgta aaccaaagaa ccataagaat ggtgtgataa
     8041 atgcaagcag taaggcagat ggataagagt ttgcaagtcc cataaatggt tcttggacca
     8101 ctgtatagaa agaacctacg atatcatcaa caccgaatgc agcaaaaatt gcagcaacta
     8161 aagcaaacgc agagatagtg atcattgctg gtagtaatga agcaaatgct tttgcaactg
     8221 ccggtggcac accatcaggc attttgatga tcaatttttc atttccgctt aaacgtacaa
     8281 agatttctgt tgaaataatc ccaacaataa tagcaataaa caaaccgttt gagctcatac
     8341 ctaatgctcc gcctaaagcg aagaaagagc ctaaagaaat cacacctgcg gaaacaccgt
     8401 cttttcggta gccgttagct aaatgataag caagcaagaa agcaatcaag acagaaagaa
     8461 tggcaaatgt accgttccaa gcagcagccc cgaatccttt ccatgattca ccaccaaaaa
     8521 ttgagttcat caattcttgg aacccgggaa tcggtaagtt attgatcatt actgctaatg
     8581 ctcctaagat cattaacggc atactcacca taaatgagtc ccgaatcgca actaaatgcc
     8641 gttgtgcacc aatttttgat gctacaggta taaaatgttt ctccataaat gcaacaaatt
     8701 tatccataaa tcttccctct tttctttact gtagattacg cgttcaatcg acgatacaga
     8761 ttaatgatct cttttgctaa atctgtaaaa gctatagatg tcatcagatg atcttgactg
     8821 tggaccgtca aaagcgtgac ttcaacgtga tttccttgtg cttcttgtgt cagcatctct
     8881 gtttgagagt gatgtgcttg gactaaagat tcctctgcat ctttgatttt ttcatctgct
     8941 aaagtgaaat cacctttttt cgcagcagca atcgcttcca ttgcatcgct ttttgcattt
     9001 ccgccaaaca tgatcagacc catgatggct tctagatttt tttgatcttc catttattgt
     9061 atcctccgat aatttttatt atttgtttat tagtttttct gcttggtcga gtactttttc
     9121 accattcatc atgccatagt cagccatatt gattacatct aaaggaatgc ctttaggtgc
     9181 tagcttttca gcaaattgat tttgcataaa gcgtacttgc ggtcctaaaa gtaaaacgtc
     9241 tacattttta gattctagtt gattatccgc atctgaagca gatacagcaa agatgtctgc
     9301 ttccattcct tttgcttctg ctgccttttg catttttgtg actaggagac ttgtactcat
     9361 tccagctgaa catacaagca tgattgtttt cttttccatt ttaaacacgt tcctttctat
     9421 acttacactt gtatataagc aagtagcgtg ccaactttaa aagcgcttta aaaagttgat
     9481 atcatcacat ttaaggtcta taccaagttg catacacact aaaaaagaaa gcgcacacta
     9541 aacacacaaa ttaattttta ttaccttttt tcttttaacg tgcacaaaaa aagttgtgac
     9601 acaaaacttg tcacaacttt tttcaaataa acggtattca aaagcttgca tctgcggtaa
     9661 taagtccaac caagttaaaa acataagatg ttgtttttac tttgctattc ttattacttg
     9721 atgcaggaca gctttttcac aacctctcaa taaacggtgt tcaatcagct gaccctgggt
     9781 attagctcaa aacgctggat tcacaacctc ttttatttca aataaccagt aaatacaatg
     9841 aacatctaca aaaaacttct atttatttat agatttcgtc aggcaaatcc aattctctct
     9901 tatcaatcaa agtctgatac cacgaagcag attttttcaa tgttctgctt cgttcatcgt
     9961 tcaattcgat tcgaactaat ccatatcgat tcttaaatgc attcattggt gacacacagt
    10021 cagtgaatgc ccataacata tagccttcgc aatttgcgcc ttcttctgtt gctttgatca
    10081 agtaatgcaa atgctgtccg ataaattcga tacggtaatc atcttgcacg attcctttct
    10141 catccatata ctgttcttca tattctcttc ccataccgtt ttctgttatc agccaaggga
    10201 tattatgata attttctttt agatacatgg ctatatcata catgattttc ggatagattt
    10261 cccatcccct ggaattattc attttcttcc caggtaaatc aaagacttca taatatcgtt
    10321 caggatggaa aggtgtctct tcattccatt gataacgagg ggcgcgaacg cgtttaggat
    10381 aatattgatt gattccaagg aaatctactg tatgatgctg aatcgtttct aaatcctctg
    10441 ttgcaggatc gaaataaatc gattgttttt gacacaactc caggaactct tctgtgtagt
    10501 atccttttac catcggatca aaaaatactc gattataaaa caaatcatac atataagcag
    10561 ctttttggtc ttctggagaa ttggatcgag catagaccat ttcaggattt aaaatacagc
    10621 caatccttcc accataattt ccgctgcggt aaatctctac tgctttagcc gttgctaaaa
    10681 ctttatgata attccacatc atccactttt ttgtgttttg ttcatgcggc cagcgaacag
    10741 catccaaata gcaacgcgtt tggggaacga ttggttcgtt gaatgtaaac cagtttttta
    10801 ccctatcacc gtaacgatca aaggcaattt gagcatactt agcataaaaa tcaactactt
    10861 ttcttgaaga ccagccatca tatttttcca aaagaaaagc tgggacttca taatgttcca
    10921 aacaaatcat cggttctacg tctgcttcga tcaaagagtc gatcatttca cttatatgct
    10981 gagcatattc ttcatctact atacagttct cataatcagt aaaaaaacga gcccaattga
    11041 tagaagtccg ataatgtgtc agatggatgg atttcatcag atccacatcc tgctggtatt
    11101 gttccataaa attagttgct acggctgggc catacccatt atgccaaaca aaatgttcat
    11161 ttttatacca gctatctata aacgaatcct gtccttcttt ttttcctttc caaccttccg
    11221 tttgccaagc agaagatgca gcaccaagaa taaaattttt tggaatagta attttcatga
    11281 tttcctcctg ttttttagat cccatcattt tccacacgat ttgctacttt tacaaatggc
    11341 agatagatca aaatagaaac aataatacaa acaagttgcg taataactgc accgatattt
    11401 cctgctgttg ccagataagc attgacgatc ggcggcattg gccaagggac cattacaact
    11461 gctttcgccg caaatcctaa agcggttgca aaataaccaa tcgttccagt gataagaggc
    11521 gttaagataa acggaatagc taaaattgga ttcagcatga tcggcatacc aaaaataact
    11581 ggttcattga tgttgaagat cccaggagca agggataact tggtgatttg tttcatatct
    11641 tcccgttttc caactaacaa tactgcaatc agtaaaccga tcgtgactcc agacccgccc
    11701 atactcatat acatatccca aaatggcata gtaataatgt tcggtacttc ttttccagct
    11761 tcaaaagctt ccgtatttac agcaattgcc gctaaaagca acggttcccg tatcggcttc
    11821 accatttgat ttccatggat accaatcacc caaaagactt gcgcaataaa catcaatagc
    11881 aaaatacccg gtaacccttg tacgacacct tctaatgggc gttgtacgac attgtaaatg
    11941 atatcataaa ggtacattcc tgtaagttct ttgatgataa atcccgcagc cgcgataccc
    12001 gatacagtca gaatcgtcgg gaataaagca gaaaaactag cagaaacatt cgttggcacg
    12061 gtatcaggca ttctcagctg tagtttcttc tgtttcctca accagcaata aagttcaata
    12121 gaaaggatgg agaccaacat tcccaaaaat aaccctttcg tatctgtata ttgtttagcc
    12181 aatacgtttt ctactataac agtctgattt tcattcacta attcataaac agttggatta
    12241 acagataaat aagacatcac tgcgatgata ccagggaata caccatccac tttattcagc
    12301 ttggctaatt cgatccctat cagaaaaaca gcatagatgg tcaagaaact tagtgtgaca
    12361 tagctgatgg cagatgcgat aggctttaat tcttctaaaa aacgcaagaa acttatctgt
    12421 gctaagccat tttccgagtc aaagaccatt gctgaaaaca atgtgccaaa agcacccgta
    12481 ataattaccg gaatcaatgc agcaaatgcg ttttttatca ccatgatata ccttaaatta
    12541 ttgattctcg ttgccacgtt ccctaaaaca ttggctaatt tatccataaa actcattcca
    12601 gatcccctca ttcctgcttc ttctccttga cgtcatctct ttttgtctat tagtcagcgc
    12661 ttacatttcg atgaacagaa actatttact cctctttttt accaaatata aaacaaaaat
    12721 tcatctgatg tatttatcat aaaatacatc agatgaactg tcaattagtt tctttttctc
    12781 ctattatttt ctgtaattct ctccgattta ttgccatatg gacagtcatt gcatcaaacg
    12841 cagcaagtgc attccgcctt tcgatggcct gaacgatctc tgtatgaata tcgatcgtct
    12901 cgattttcag atgctggata tcataatttt tattaaacaa agcaattgat tcattaatga
    12961 ttgggataat atgcagcaag gctacgtttc cgctagcttc tgcaatcatc gtatgaaaat
    13021 cgatgtctaa ttgaacatgt tcttgtgtgc cttctcgaaa agaagtttca atcttttgcg
    13081 ttaatttttt catttggacg atttgattct tcgttgcata ttccgctgcc aaacctgcca
    13141 tcttaggctc caataaatat ctcagttcaa ataagtctcg tatcatccga tcctgatctt
    13201 caatcaaaga aaaaccgagt gggtcattgg caatccctcg cttatcactg acaaatgtcc
    13261 ccgctccttg ccgaacttca aggatattcc tggaagctag agcccgcaca gcttctcgca
    13321 ccgtgcttcg ccccacctct aaataatcgg acaattcata ttcattcggc agtttatctc
    13381 ctacttgcaa ctgcatttcc tttaaatatc taaggatctt gtcagtagtt acttctacta
    13441 gggttttatt ttcttccaaa ccacatacct ccttttcatc tgatgtattt tttcgtaatt
    13501 attataacac gtttgaatca tctgatgatt ttttgtatac aaaattgatt aatccgttca
    13561 tagacataca aacgaagaag atatttacca taaaaaagac taggataaaa ttcactccta
    13621 gccttcgatc atttttattt tgaacgacaa aaaatatgat gttcaataac agacgatctt
    13681 cattcgattc ttgatgtcat cttttgtttt ttttaatata tggataaaaa gtttgattac
    13741 atcgtttcta ttctagaatt tggtcgattc tcaattcttc ttctggtgag aaatctaaat
    13801 tatccatcat tcgaacactt tcttgtaact gacttaaccg acttgctccg accagtacac
    13861 ttgtaacaga tttttgtcgc aaattccatg caagggccat ttgcgctaat gattgtcctc
    13921 gtgatacggc gattgtttgc agtgctttga ttttttccag agttgatcca acttgttctt
    13981 ctgaaaggaa tggtatttcc ttacgatgtg ctctagaatc ttcaggaata ccatgtagat
    14041 aacgatttgt cagcaaccct tgagcaagcg gactgaacgt gattgcccct agcccttctt
    14101 cttctaatac tggagataga tcgtcttcta gtagtgcacg agaaaacata ttgtagcgca
    14161 tctgatgaat aatgaacggt gcttcctttc tttttaaaat ttcagtcatt ttttttgtat
    14221 cttctccatt atagttggat ataccaatat ataatgcttt tccttgtctt acaagttgca
    14281 tcaaagcttc tgctgtttct tccaaaggtg tatcagggtc tgggcgatga tgatagaaga
    14341 tgtcaacata atctagcccc attctttgga gactttggtc acaacttgaa atgatggatt
    14401 tacgagagcc ccattcccca tatggccctt cccacatatg ataacctgct ttgctagaaa
    14461 taatcagttc atcacggtag gcttgaaaat cttctctaaa aatacgccca aagtttttct
    14521 ctgcagctcc agcagggcct ccataattat tcgcaaggtc gaaatgggta attcccatgt
    14581 cgaatgcgcc tcttaaaatc tctctttgat tatggatagg atcataatca ccaaaatttt
    14641 gccataatcc gagagataaa atcggtaatt tcaaacctga atgaccggat ttacgatatt
    14701 ccattttttc ataacgtgca ctatctgcaa tatacaattt ttctcctcct catcaaatct
    14761 ttttaaaacg ctttcttttt tctattttta gtttactaca ttttaaagaa aaggagttaa
    14821 tcaaaatgat tttttcgtat atttttttgc catttgtttt ttactcgttg atagatcaga
    14881 aattgattac gtgcttgctg gattttttta taacgtttcg gttgtttttt gtaaaattgc
    14941 tgatgatagt tttctgctgg ccaaaacgta ccggctggtt cgatttttgt gacgattggt
    15001 tggtggaaag ttcctgaact gtctagtttc tgttttgatt gttccgctaa ttctttttgt
    15061 cgttcgactt gataaaaaat cactggacga tattgcttcc ctctatcttg aaattgtcca
    15121 aatgtatctg tgggatcagt aatttgccaa tagattgtca gcaactcgct ataacttatt
    15181 tctcttgtgt cataaatgat ctccacagct tccacatgac ctgtgtaacc accacttact
    15241 tgatcataat ttggtttttc aacatgtccg cccgtgtatc ctgacaaaac agataggatc
    15301 ccttttttcg attcaaacgg ctcgaccata caccagaaac atccccctgc aaatactgca
    15361 cgttcttgat agtcagcatc ttgcatggca tgcttggaaa agtcatattt ttgctcctta
    15421 tcaaatcctt ccgtaatcaa taaataaaag tccattacat caggcgtcag attcattcgg
    15481 atagctaatg gacgtagtgt tgcttctagt tttgataact gttctttaac ggatatattt
    15541 tctcttatgt cattctttgt ctgaatcaat acttttcgtt cccaatctcg tgtattcgga
    15601 ttcaaaatca gattataaag tgtttgaatg aatatttgtt cctcatccat agattctcct
    15661 cctttccgaa gaagacaagc tagtgacttt agctgtaatt tatcataaag tgaggaaata
    15721 agcgattact tcatccccct acagattttt cgtataaaaa agagagaatc cttatcgaca
    15781 gattctcctt tttaatcaat ttattttata aatgaaaagt taatttacct tttatggaaa
    15841 actatt
//
LOCUS       DACDKM010000066        15670 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20834_2016
            SAMN12640370-rid9891663.denovo.066, whole genome shotgun sequence.
ACCESSION   DACDKM010000066 DACDKM010000000
VERSION     DACDKM010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640370
            Sequence Read Archive: SRR10040891
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15670)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15670)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742835.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742835.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:04:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,012
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 29 of 75
            Pseudo Genes (incomplete)         :: 33 of 75
            Pseudo Genes (internal stop)      :: 19 of 75
            Pseudo Genes (multiple problems)  :: 5 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15670
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640370-rid9891663.denovo.066"
                     /isolate="E20834_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(<1..1158)
                     /locus_tag="IV334_001847"
     CDS             complement(<1..1158)
                     /locus_tag="IV334_001847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HAP8943911.1"
                     /translation="MKIEIIIVHLSCIQEVKIMGIEFSERQMKILYFLIENEHTTYKE
                     LSEQLYVSTKTISKDILILKEIIQKMPDVQMLVQPKKGVSLIGKKETLRNLLSSHKIN
                     IADSPEDRLTYVFAKLLNTNDSLKIQNLADELYVSRSTVESTMKDVRKRLNKHGMSLV
                     SDRNGVRIKADEKSKRRLMSEVVNYYWGGFTASNSKTDLLDLNINFIGEANNFLNKDI
                     LKKVAELLNRYMNKTQLKFTDYEFQTLAIHLAIAIERIETSNFLSRSNNSEEEISKNT
                     LILIKMIEKAFSIIVPIDEQAYVDIHITSIEKNAMNHDLDQTKLSYLDMGVELQELVR
                     IKLNEFYPDEELINNLVLHLNAAVKRLKLGVNIYNPYTDKIKYSFKRSFMIS"
     gene            complement(1316..2212)
                     /locus_tag="IV334_001848"
     CDS             complement(1316..2212)
                     /locus_tag="IV334_001848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAP8943912.1"
                     /translation="MIKWLLLLVVIFMGSFIFFLQREIKRLTKKIATLSERQEYGGRL
                     SVEFREKNLMDLVDALNTWLDENEQKGQAFQEMEENIRLSIVGLSHDLRTPLTAINGY
                     VQLLEQVEDTEKREKYLTIIKQSVARLLSMTDQFYDLARIETKQKEMDLHTLSFSKEV
                     EEVFFSFYEQFSEKNIEVAFAEHSINTTVLADPLMLLRVLQNIIQNLLRYADGQAKVT
                     FSKEDPFIRLTVANPIKPGTQTSVEKVFQRFYTENTSRTNTESSGIGLYLSKKLVEGM
                     GGEMTARLDGGIFSISVKLRRV"
     gene            complement(2205..2885)
                     /locus_tag="IV334_001849"
     CDS             complement(2205..2885)
                     /locus_tag="IV334_001849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP8943913.1"
                     /translation="MVLLETILIIEDDEAVHSLLEEVLEQRYKLLDAYSGTEGKLLLS
                     TYPVDLILLDLMLPGLSGEALLAEIRQTSNVPIIVLSAKSDQRDKVSLLAAGADDYVT
                     KPFDIEELLLRIGIQLRHQTSVQVKDLSQRIYYKEILLDKESRDVNVNGVAVHLTGRE
                     FDLLKLFLEHPKKVFSRANLYETVWQEPYFDSEKTVNMHISNLRGKLAKAHAVYIHTV
                     WGVGFRFD"
     gene            complement(2886..3668)
                     /locus_tag="IV334_001850"
     CDS             complement(2886..3668)
                     /locus_tag="IV334_001850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HAP8943914.1"
                     /translation="MFNLLTAEKIKVWKSRKMWVSLGLMVVLPLFYSWNEWYMHTKYG
                     NELNQATDTVINGATGILMVEKMAAWILLAFAAFACFYIGEEFQNGTIRNTLSLGRNR
                     MTYYVSKLLVTLLITTLGVVLITGLAMLAYTLAFGFGEVEGIKNYGNYVLKVFPVLLL
                     LTLGTLSVPVALTFITRSTSVSLLLSFLYIMGTAFIPGVFAKIKGLEFLTEWFTETWL
                     MYTDFAQQATYSQAPKMILVSLVTIVVSSALGMFIFQKSDIK"
     gene            complement(3661..4578)
                     /locus_tag="IV334_001851"
     CDS             complement(3661..4578)
                     /locus_tag="IV334_001851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAP8943915.1"
                     /translation="MDFGNETVLKATGITKKYGAAKALDKVSIEIKRGMIYGLIGENG
                     AGKSTFMRTIMGLISIDEGSIELFGTTDLQAARRRMGQSIETPALYPELTARDNLRIQ
                     AANGGVSDREIEDLLKMMRLENTGKKKAKNFSLGMRQRLAIANALITNPEFLILDEPT
                     NGMDPAGMAEMREIIQRLVKERGITVLLSSHLLDELSQIATHYGILHEGHLIKELSKE
                     ELAQESRQFIKIDTSATEQAVTVLDSLGYRDYFVQSSRVIQLFEGIDQVAAINQALVE
                     AKVPVDGIHLVGQKLEDYFLQLTGGNPHV"
     gene            complement(4742..5065)
                     /locus_tag="IV334_001852"
     CDS             complement(4742..5065)
                     /locus_tag="IV334_001852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP8943916.1"
                     /translation="MFSFAFYMVWLQREVPTHIQGRLMSTITTITMVASPLGLALFGA
                     LFDWKTTQPRLRDSLIFLGGSLAIVILITGLVKLLKLDLKEAKIFSEEEYRETTLVEK
                     KESGN"
     gene            5853..9080
                     /gene="fss3"
                     /locus_tag="IV334_001853"
     CDS             5853..9080
                     /gene="fss3"
                     /locus_tag="IV334_001853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibrinogen-binding MSCRAMM adhesin Fss3"
                     /protein_id="HAP8943917.1"
                     /translation="MYKKQKKKLCVLTILSLAVQAIVGAIAPTITFADEITHPQTVTV
                     ELDLAHQYAVEGTFSDGRPMSEVTVPHYAVYNGFKQDIFCIEPGVPIYNEFTPGYEKN
                     PLPDMSDKAKLVSVLWKKVGTDADTHIVAQKMIWQEVNGYTLHSIKRSDGSAVNIAAI
                     EAKINQAIDDYQKKPTFHNSTAKTVLGQSTTVTDTNNLNLSEFDEVVENAANIDYRVN
                     GNQLIITPNANSKESGVLTLKKSAGTGTPVAYKKAGQQTLMAGAIDKPNTYTIKIDVE
                     TEGSLKIKKVDKESGDVVPGTVFHLDFGKTLPAKDVTTDKEGIATLDEIPHGTKVTIT
                     EKSVPAPYTIDTTPMTTTIKAGETIYVTSKNAREKGQIILDKSGVETGSDLWNDNYSL
                     AGNTFAIRKDSPTGEIVQEMTTDENGHAETPKEIANALELGTYYVTETKASHGFVNTF
                     KPVKVELKYANQTVAPVTSNVKGQNQEVTGETTLTKEDKDTGDKAQGKAVFEGAEYTL
                     FTAKDGKAVKWSEAFKPELAKGTKGSDETVTLTLDEKNQVAVKHLAINEYYWQETKAP
                     EGYSLDETKYPVSIKKVDDSEKNAVITRNVTAKEQVIRFGFDFFKFAGSAAGTTETGF
                     NDLTFKVSPLEGTNEITGAEDEATTAYNEQLGFDGYGKFENLPYGDYLLEEVEAPEGF
                     QKITPLEIRSTFKENKEDFVKSEYVFTITEQDQKQPIKTVTVPYEKLTNKAFSVSLNR
                     LMLYDLPEEEDSLTSLATWKDGNKELTSLDSTELVDKLSYNLHEIKEDWYVVAQAIDV
                     DATKAAQEKDEKAKPVVIAETSATLANKEKTGTWKIQHKLTAEQVLNKTIVLFNYVYE
                     NKEAFEAGDKPVAKDVSLNNQAQTVSCTVEHHVSIQTKAHLENGSQTFTHGDVVDMFD
                     DVSITHDVLDGSKEAFETILYALLPDGTNKEIWKSGKIDYEVNDKEFTKTVLAKKVDT
                     GKYPEGTKFTFAEINYDKDGNINGKHNEDLKEKSQTLTPKEVPTILSTPKQPETPTVP
                     SGSQESSPTVKTFPQTGEKNSNVLLFIGFTLIFATAGYYFWNRRN"
     gene            9193..9507
                     /locus_tag="IV334_001854"
     CDS             9193..9507
                     /locus_tag="IV334_001854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HAP8943918.1"
                     /translation="MELKFVVPDMAETFGKLSYAGEGEIITEGYGRNTTVIGRSYHLY
                     SSKQRADDIEVVVAAEAGEKDFDQDQPLKAVNPHLVAKGYEIENRGFTDYVLYVDDLV
                     KA"
     gene            9520..9894
                     /locus_tag="IV334_001855"
     CDS             9520..9894
                     /locus_tag="IV334_001855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HAP8943919.1"
                     /translation="MRLTEGIVVDSGLTFGKLRFSALRREVRKQNEDGTIGNEVKERT
                     YNLKSSAQGRMIQVSIPANVPLREFAYDAEVELVNPIVDTVANYVFREGTTVNWFIKA
                     DDLVLKRQPNQGNPTNQNERKK"
     gene            9904..10239
                     /locus_tag="IV334_001856"
     CDS             9904..10239
                     /locus_tag="IV334_001856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="damage-inducible protein J"
                     /protein_id="HAP8943920.1"
                     /translation="MEINVEHILDCLDQYGKGELTEEQLVKALTSDEKMFLIIHQGLL
                     GENSDSEEDSDVLDWLGEEESFFIVVEVNENLCRQAESILEEIGVEMPDAIEGFLNQL
                     VETKQLPSS"
     gene            10322..11662
                     /locus_tag="IV334_001857"
     CDS             10322..11662
                     /locus_tag="IV334_001857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010759325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF87 domain-containing protein"
                     /protein_id="HAP8943921.1"
                     /translation="MYKGHRIRAGDQHLVYHFVLGWLLALFIGWMSVFYFQEFRQFDI
                     SKLSLSTIEIVWSIKDLVCLLGSLAFSGAMILLYIHFFLDHWRSLWHRQKLARMILEN
                     HWYEVKQTQSEGFFKDLNSSRTRETISYFPKICYRMKDGLLSIRVQISLGKYQDQLLK
                     LEKKLESGLYCELVEKELKDSYVEYTLLYDMIANRIGIDEVVAENGTLRLMKNQVWAY
                     DSLPHMLIAGGTGGGKTYFLLTIIEALLKSDAELFILDPKNADLADLGTVMPHVYSQK
                     EEISACVEDFYERMMARSKAMKEMSNYKTGENYAYLGLPPNFLIFDEYVAYMEMLTTK
                     ESAVILNKLKQIVMLGRQSGFFLILACQRPDAKYLGDGIRDQFNFRVALGRMSELGYS
                     MMFGEVEKNFFMKHIKGRGYVDTGGSVISEFYTPLVPKGYDFLDSISKLEKMND"
     gene            11740..12414
                     /locus_tag="IV334_001858"
     CDS             11740..12414
                     /locus_tag="IV334_001858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAP8943922.1"
                     /translation="MESNDSYLEYAKANKDKFISYIIKGKSPEVGRDAVFMAGSPGAG
                     KSEVAIGLAENYDNHVIIDADYFRTQFPDYNGKNSSLFQKASSWLVEQAFRYVLDHGY
                     SFILDATFAILSAEKNISRALKKHFRVTIFYVYQDPKIAWKFTQEREIAEGRHVPKKA
                     FINAFFKSRENIAKVKSKHPDVLLHIIIKDYTNNISEVHYATDNLELFLPLGYSRKDL
                     EVQLHD"
     gene            12647..13081
                     /gene="dcm"
                     /locus_tag="IV334_001859"
     CDS             12647..13081
                     /gene="dcm"
                     /locus_tag="IV334_001859"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA (cytosine-5-)-methyltransferase"
                     /protein_id="HAP8943923.1"
                     /translation="MRFLDLFAGIGGFRLGMEQASHHCIGFCEIDKFARRSYKAIHDT
                     SKEVEMHDITSVSDEFIQSLGPVDILCGGFPCQAFSIAGKRQRFSDTRGTLFFEIARF
                     TTLLRPKLLFLENVRGLLNHEGGATFETILRTLDGLGYDVEW"
     gene            13082..13789
                     /locus_tag="IV334_001860"
     CDS             13082..13789
                     /locus_tag="IV334_001860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA (cytosine-5-)-methyltransferase"
                     /protein_id="HAP8943924.1"
                     /translation="MLNSKAYIPQSRERIFLIGHSRDACTEQVFPIIGPSSTSDQNIR
                     NLVNINPSNRGMGGQVYGSDGVAPTLTGDEGIKIALPARDGISVAGLLPGKFEQGNRV
                     YEVTGTAPTLSTKQGGTKIMIRKNETEYQEVKPEDSVNLAFPNSTSRRGRLGKQSVHT
                     LLTGDQQAIVTDQYQIRKLTPRECWRLQGFPDWAFDRDSQVNSDSQLYKQAGNSVTVP
                     VIFDIARRLKELDVHGL"
     gene            13779..14069
                     /locus_tag="IV334_001861"
     CDS             13779..14069
                     /locus_tag="IV334_001861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002395166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8943925.1"
                     /translation="MVYDELIGEIYWVIGKIQSDPELEEQLRRLKFDIRKNGVKVAGD
                     PYLMNEETDARIEINQVIAEFERIADQAKEPDIRQYLLEMKAELEVNGITDE"
     gene            14328..15512
                     /locus_tag="IV334_001862"
     CDS             14328..15512
                     /locus_tag="IV334_001862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HAP8943926.1"
                     /translation="MAQRNLDYRLLKDRRNEYGVSQNKLATACGLSRPYLNQIENGGV
                     TASIKTMRKIFDQLESFNPDLPLTLLFDYVRIRFPTTDARKIIQEILHLKFEYMLHED
                     YAFYSYQEQYVMGDIVVMLSHEEDKGVLLELKGRGCRQFETFLLAQKRSWYDFFEDCL
                     KAGGVMKRLDLAINDRVGLLDIPELTKKCQKEECISLFRTFKSYRSGELLKADEKDGM
                     GNTLYIGSLKSEVYFCLYEKDYEQYIKLGIPLDQTETKNRFEIRLKNDRAFHAIQDLL
                     KGRSIESTTFSIINRYLRFADKVEGKRRTNWPLNEQWGRFIGRNRKEIQLTSEPKPYT
                     IERTLNWLGRQVAPTWKMAKELDRLNRTTYIQDMVQNARLSDRHKKILEQQSMAIENL
                     II"
     gene            15527..15646
                     /locus_tag="IV334_001863"
     CDS             15527..15646
                     /locus_tag="IV334_001863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3789 domain-containing protein"
                     /protein_id="HAP8943927.1"
                     /translation="MGIVKDIFLVFGGSMLGVTVMCLMNASAAADRAMEKKGK"
BASE COUNT         4987 a         2846 c         3194 g         4643 t
ORIGIN      
        1 acttatcata aatgaccttt taaaagaata tttgattttg tccgtataag ggttatagat
       61 atttactccc aatttgagtc ttttaactgc tgcattgaga tgtaatacta gattattgat
      121 caattcttca tcaggataaa attcatttaa tttaattctt actaactctt gtagctcaac
      181 acccatatct aaataggaaa gcttcgtttg atctaagtcg tgattcatag catttttttc
      241 aatcgaagta atgtgaatgt caacataagc ttgttcatct attggcacaa taattgaaaa
      301 tgctttttca atcattttga ttaaaataag tgtattcttt gagatttctt cttcagaatt
      361 attactacgt gataagaaat tgcttgtttc aatcctttct atcgcaatag caaggtggat
      421 agctaaagtt tgaaattcat aatctgtaaa tttcaattgt gtcttattca tgtatcgatt
      481 aagcaattcg gctacttttt tcaatatgtc cttatttaag aaattattcg cttcaccaat
      541 gaaattgata ttcaagtcta agagatctgt tttactgtta gatgctgtaa agccacccca
      601 ataatagttc acaacttctg acatcaacct tcttttactt ttttcatcag ctttgatgcg
      661 cacgccattt cgatctgaaa ctaaggacat cccatgcttg tttagtcttt ttctaacatc
      721 tttcattgtg ctttctaccg tcgacctgct cacgtataat tcgtcagcaa gattttgaat
      781 ctttagagaa tcattagtat tcaataattt agcaaacaca taagttagac ggtcctccgg
      841 agagtctgcg atattgattt tatgactgga taataaattt cgaagtgttt ctttttttcc
      901 aataagcgaa acgccttttt taggttgaac caacatttgt acatctggca ttttttggat
      961 tatttctttc aaaattaaaa tatctttgct aattgttttc gtagatacat ataattgttc
     1021 tgataattct ttgtaagttg tgtgctcatt ttcaatcaaa aagtatagta ttttcatttg
     1081 ccgttcgcta aattcgatgc ccattatctt cacctcctga atacagctta agtgtacaat
     1141 tattatctct attttcatac agaagaaagt tcttgattaa tgtagaagtt tctaatattc
     1201 ttgattattt caaatcaata aaaaagcctg caatatttat atcacaggct tttttctcta
     1261 ttaaatatat ataagataat acatttttct actagttctc actaatcttg tataattaaa
     1321 ccctccgcaa cttgacggag atggagaaga tgcccccgtc taatctagcg gtcatctctc
     1381 ctcccattcc ttccaccaat ttttttgaca ggtaaagccc gattccgcta gattccgtat
     1441 ttgtgcggct ggtattttcg gtataaaaac gctggaagac tttttcaaca gaggtttgcg
     1501 ttcctggttt gataggattg gccactgtta atcgaatgaa tggatcctct tttgaaaatg
     1561 tcacctttgc ttgtccatct gcataacgca ataaattctg tatgatattt tgcagcactc
     1621 gcagcaacat gagcggatct gctaatacag ttgtatttat cgagtgttca gcgaacgcta
     1681 cctcgatatt tttctcagaa aattgttcat aaaaagagaa aaatacttct tccacctctt
     1741 tagaaaaaga aagcgtatgg agatccattt ctttttgctt cgtctctatt cgagcaagat
     1801 cataaaactg atccgtcata ctgagtaatc ttgcaacaga ttgtttgatg atcgttaaat
     1861 acttctcccg tttctccgta tcctctacct gttccagtaa ttgtacataa ccgttaatag
     1921 cagtcaaagg agttctgaga tcatgagaaa ggccgacgat cgatagtctg atgttttctt
     1981 ccatttcttg aaatgcttgt cctttttgtt cattttcatc caaccatgta ttcagtgcat
     2041 ctactaggtc cattagattc ttttctcgaa attcgactga aagacgacca ccatattctt
     2101 gtcgttcaga aagagtggcg attttcttag ttaatctctt gatttccctt tgcaggaaga
     2161 aaataaaact tcccataaag ataacaacga gaagcaacag ccatttaatc aaatctaaac
     2221 cccactcccc acaccgtatg aatatacaca gcatgtgcct ttgctagctt ccctcgaaga
     2281 ttggaaatat gcatattcac tgttttctca ctatcaaaat atggttcttg ccaaactgtt
     2341 tcataaagat ttgctcgcga aaatactttt ttcggatgtt ctaaaaaaag cttcagcaga
     2401 tcgaattctc ttcctgtcaa atggacagcc acaccattca catttacatc cctcgactct
     2461 ttgtccagta agatttcctt gtaatagatg cgttgcgaca gatcttttac ttggacagaa
     2521 gtctgatgac gaagttggat gccgattcgt aacaaaagtt cttcgatatc aaatggtttc
     2581 gtcacatagt catccgctcc tgctgccaac aaggaaactt tatctctttg atcgcttttt
     2641 gctgaaagaa cgatgatcgg aacattcgat gtctgtcgga tttctgctaa caatgcttca
     2701 cctgaaagcc ctggaagcat gagatctaga aggatcaaat cgacaggata tgtgcttaac
     2761 aataattttc cttccgtacc tgaataagca tcaagaagtt tgtatctctg ttcaagaacc
     2821 tcctccagta aggaatgaac agcttcatcg tcttcgataa tcaaaatagt ttccagcagg
     2881 accacctact taatgtcaga tttttggaaa ataaacattc ctagagctga tgataccacg
     2941 attgttacta gactgaccag aatcattttc ggtgcttgcg aataagttgc ttgttgcgca
     3001 aagtccgtat acatgagcca cgtttccgta aaccactccg ttagaaattc caagcctttg
     3061 atttttgcga aaacaccggg aatgaaggcc gttcccatga tataaagaaa agaaagaagc
     3121 aaactgacac tggtacttct agtgatgaat gtcaaagcca ctggaacaga taatgtccct
     3181 aatgtcagca gaagcaatac aggaaatact tttagaacat aatttccata gtttttgatt
     3241 ccctcaactt caccaaatcc gaaagcaagt gtgtaagcaa gcatggctag tcccgtgatc
     3301 aaaaccacgc caagtgttgt gatcaaaagc gtaacgagaa gtttcgacac ataataagtc
     3361 attcgattcc gtcctaaaga taacgtattg cgaatcgtac cgttttgaaa ttcttcccca
     3421 atataaaagc acgcaaatgc tgcgaaagcc agaagaatcc atgcagccat tttttctacc
     3481 attaaaatcc ctgttgcacc gttgatgact gtatccgttg cttggtttaa ttcattgccg
     3541 tattttgtat gcatatacca ttcgttccaa gaataaaaaa gcggaagtac gaccatcagt
     3601 cctagtgaga cccacatttt acggctcttc catactttta ttttttcagc agttaataga
     3661 ttaaacatgt ggattgcctc cagttaattg taagaaataa tcttccagtt tttgcccaac
     3721 taggtgaata ccatccacag gaacttttgc ttctaccaat gcttgattga tggctgctac
     3781 ttggtcaatt ccttcaaaaa gctggataac acggctagat tgtacaaaat aatccctata
     3841 accaagagag tcgagaaccg ttactgcttg ttccgtcgcc gaagtgtcaa tttttataaa
     3901 ttgacgagat tcttgtgcca attcttcttt cgataattct ttgatcaagt gtccttcatg
     3961 caaaatacca tagtgagtgg cgatctgtga caattcatct agcaagtggc tagaaagcaa
     4021 gacggtgatg ccccgttctt ttaccaaacg ttggatgatt tctcgcattt ctgccatccc
     4081 tgctggatcc atcccattcg tcggttcatc caaaatcaaa aattctggat tcgtgatcag
     4141 tgcatttgca atagctaaac gctggcgcat acctaaagag aaattctttg ctttcttttt
     4201 ccctgtattt tccaatcgca tcattttcag caggtcttcg atttctctat ccgacacacc
     4261 gccgttcgct gcttggatac gtaggttatc tcttgcagtt aattctggat aaagagcagg
     4321 ggtttcaatc gattgaccca tacgtcgtct tgctgcctgc aaatccgtcg ttccaaataa
     4381 ttcgatcgaa ccttcatcaa tcgagatcaa acccataata gttctcataa aggtagactt
     4441 acccgctcca ttttccccaa tcaatccata gatcattcca cgtttgattt cgattgatac
     4501 tttatctaat gctttggcag caccgtattt tttggttata ccagttgctt tcaagaccgt
     4561 ttcatttcca aaatccatac ttatccttct ttcttattga tgagtgaagt atagttgacg
     4621 ggtataaaga aagcagccag aaatgtataa agaaagtgta aattttccaa ttgaatgtca
     4681 aaaaaaacaa tcggacaata cgtgtctttc ctattatcag gcagtttgtt atacgcttgt
     4741 atcagtttcc tgattctttc ttttcgacca aagtcgtttc tctgtattcc tcttcactga
     4801 aaatttttgc ttctttcaga tcaagcttga gtaattttac cagaccggtt ataaggataa
     4861 ctatagcaag actccctccc agaaaaatta gactgtctcg cagacgtggt tgcgttgtct
     4921 tccaatcaaa gagcgcacca aacagtgcta atccaagtgg actggccacc atggtaatcg
     4981 ttgttatcgt tgacataagt cgtccttgta tatgcgttgg aacttctcgc tgaagccaga
     5041 ccatataaaa agcaaaagaa aacattaatc cttaaaaacg gacttatgaa aaataataca
     5101 tgccgtttgg gatttacaat tagctaccaa ataagaggtt atgacaaaag acttttcaca
     5161 atcccttctt ccgaataaac ggtgttctcc tgccaactcc tcggtcacaa gtcacaatat
     5221 ttatcaaaca tgagaagcaa tttttgtaaa ttccttctta tttcttgaag ctgaccactt
     5281 ctgtcacaac ctcagaagcg gataaataat attgtttgat gatactttat ctttttcccg
     5341 tttgtttata gatcgaatga tattatagac caagaatcct acctattaac atagctaaaa
     5401 acacaaaaaa ggcactttcc aaattgtgga aagtgccaga aaagttagga ttaaggtaac
     5461 tgcgctcagc attgcttcgc ccagtacgat tctcgtaact cgtgaactcg aacgataatc
     5521 gcaattaacg ttttgagaac tgtgaagctt tacgggcttt cttaagacct ggttttttct
     5581 tcgtatcatt gaataagttt gaaaaacctt gatttataaa ggttttcccg taaatatatt
     5641 ttattatatt tgattaaatt tgaaatgttt gtgttctaaa atgtgttcta atttaaaggc
     5701 agtatatttc gtattttaga ttaaattttt aggctcattc attctcaaat ccattctatt
     5761 cagtcaataa aaattttaaa aagactttat aaacaaagat gttcaagcga gcatcttatt
     5821 tttttgtgct taatttgaaa ggagaacatt tcatgtataa aaaacaaaaa aagaaattat
     5881 gtgtattaac tattttgagt ttggctgttc aagcaattgt tggagcaatt gctcctacga
     5941 ttacttttgc tgatgaaatt acccatccac aaacggtaac cgtagaacta gatttagcac
     6001 atcaatatgc tgttgaagga acttttagtg atggtcgtcc aatgtcagaa gtcactgttc
     6061 cgcattatgc agtgtacaac ggctttaaac aagatatttt ctgtattgaa ccaggagtcc
     6121 cgatttacaa tgagttcact cctggctatg agaaaaaccc gttaccagat atgtccgata
     6181 aagccaaatt agtttccgtt ttatggaaaa aagtaggtac agatgcagat actcatattg
     6241 tggcacagaa gatgatctgg caagaagtta atgggtatac ccttcactca ataaaacgat
     6301 cagatggtag tgccgtaaat atcgcagcca ttgaggcaaa aataaatcaa gccattgacg
     6361 attatcagaa gaaaccaacc ttccataata gtacggctaa aacagtatta gggcaatcga
     6421 ctactgtgac ggatacgaat aatctgaatt tgtcagagtt cgatgaggtt gtggaaaatg
     6481 ccgcaaacat tgattatcgt gtgaatggta atcagttgat aatcacccca aatgccaatt
     6541 caaaagaaag tggtgtgctg acgcttaaga aatctgctgg taccggaaca cctgtcgcct
     6601 ataaaaaggc tggccaacag actttaatgg ccggggcgat tgataaacct aatacctaca
     6661 caatcaaaat tgatgtggaa acggagggtt ctttaaagat caaaaaagtc gataaagaat
     6721 cgggtgatgt tgtaccagga acggttttcc atttagactt tggaaaaact ttacctgcaa
     6781 aagacgtgac tactgacaaa gaaggcattg ctacattgga tgagattcct catggtacaa
     6841 aagtaaccat taccgaaaaa tcagtaccgg cgccttatac gattgatacc acccccatga
     6901 ctaccaccat taaagcaggc gaaaccattt acgtgacttc aaaaaacgca cgggaaaaag
     6961 ggcaaatcat tctggataaa agcggagttg aaacagggag tgatctttgg aatgacaatt
     7021 attccttagc tggtaatacg tttgccattc gcaaagacag tccaaccggt gaaatcgttc
     7081 aagaaatgac cacggatgaa aacggtcatg cggaaacacc aaaagagatt gctaatgcgt
     7141 tggaattggg aacctattat gtgaccgaaa ctaaagctag tcatgggttc gtgaatacgt
     7201 tcaaacctgt aaaagtcgaa ttgaagtatg ccaatcaaac cgtagcccct gttaccagta
     7261 acgtaaaagg gcaaaatcaa gaagtcactg gcgaaaccac tttgacgaaa gaagacaaag
     7321 atactgggga taaagcacaa ggtaaggcag tctttgaggg tgctgaatac accttgttta
     7381 ctgctaagga tggaaaagct gttaaatgga gtgaggcttt taaaccagag ttagcgaagg
     7441 gaacgaaagg ttctgatgaa acggtcactt tgactttaga cgaaaagaac caagttgccg
     7501 ttaaacattt agctatcaac gagtattatt ggcaagaaac aaaagcgccc gaaggatata
     7561 gccttgatga aacaaagtat cctgtatcca tcaaaaaggt ggatgatagc gaaaaaaatg
     7621 ccgtgattac ccgaaatgtt acggcaaaag aacaagtgat tcgctttggc tttgatttct
     7681 ttaaatttgc tggatcagct gcgggcacta ccgaaactgg cttcaatgat ctgaccttta
     7741 aagtgtcgcc attggaaggg accaatgaaa tcacaggtgc cgaagatgaa gcgaccacag
     7801 cttataacga gcaattaggg tttgatggtt atggcaagtt tgaaaatctt ccttatgggg
     7861 attatttact tgaagaagta gaagccccag aaggttttca aaagattacg ccattagaaa
     7921 tccgttctac ttttaaggaa aacaaagaag actttgtgaa gagtgagtat gtctttacca
     7981 ttaccgaaca agaccaaaaa caaccaatta aaacggtgac cgttccttac gagaagctga
     8041 cgaacaaagc attttctgtt agtttgaacc gtttaatgct ctatgatttg cctgaggaag
     8101 aagatagttt aacttctctt gcgacttgga aagacggaaa caaagaatta acttctctgg
     8161 attctaccga gctagttgat aaattgagtt acaatctaca tgaaatcaaa gaagactggt
     8221 atgtggtagc gcaagccatt gatgtggatg cgacaaaagc tgcccaagaa aaagatgaaa
     8281 aagccaaacc agtggtgatt gcagaaacaa gcgcaacgtt ggcgaacaaa gagaaaacag
     8341 gaacttggaa aattcagcat aaattaaccg ccgaacaagt tttaaataaa actattgtct
     8401 tattcaacta tgtgtatgaa aataaggaag cttttgaagc aggcgataag ccagtagcga
     8461 aggatgttag cttgaataac caagcccaaa ctgtcagttg tacagtggaa caccatgttt
     8521 ctatccaaac aaaggcccac ctagaaaacg gttctcagac ctttactcat ggtgatgtgg
     8581 tggatatgtt tgatgatgtg tcgattactc atgatgtact ggatggctca aaagaagctt
     8641 tcgaaacaat tctgtatgct ttattaccgg atggtacaaa caaagaaatt tggaaatctg
     8701 gcaagattga ttatgaagta aatgataaag aattcaccaa aactgtgctt gcgaaaaaag
     8761 tagataccgg aaaatatcca gaaggaacca agtttacttt tgcggaaatc aattacgata
     8821 aagatgggaa tatcaatgga aaacacaatg aagatttgaa agaaaaatct caaaccttaa
     8881 caccaaaaga agttccaacc atactaagta cgccgaaaca accggaaaca ccaactgttc
     8941 caagtggctc tcaagaatct agtcccacag tgaagacctt ccctcaaacc ggggagaaaa
     9001 attccaatgt tttactgttc attggtttta ctttgatttt tgcgacggcg ggctattatt
     9061 tctggaatcg ccggaactaa ggtgatgaga tgatacaagt aaatcctaag aaccaacgtg
     9121 cgcgaccgcc tcctaaaaaa gtagtcgcgc tatttttgta tccaaaacta acaatgaaaa
     9181 gaggaaaaga agatggaatt gaaatttgtc gtgccagata tggctgaaac ctttggaaag
     9241 ttaagttatg ccggtgaagg agaaattata acagaaggat atggacggaa tactacggtg
     9301 attggtcgca gttatcattt gtattccagt aagcaacggg ccgatgatat tgaggtggta
     9361 gtagctgcgg aagccggtga aaaggatttt gatcaagacc aaccgctaaa agccgtgaat
     9421 ccccatttgg ttgccaaagg ctatgagatt gagaatcgtg ggtttactga ttacgtgttg
     9481 tatgttgatg atttagtgaa agcataggag gaaaagaaga tgagattaac agaaggtatt
     9541 gtcgtagatt caggtttaac atttgggaag ttgcgttttt ccgcattacg ccgagaagta
     9601 cggaagcaaa atgaggatgg aacaattggt aatgaggtaa aagaacgaac ctataattta
     9661 aaatcctctg cacaagggcg aatgattcaa gttagtatcc cagctaatgt acccttacgc
     9721 gagtttgctt atgatgcaga ggtagagttg gtcaatccaa tcgtggatac cgttgccaat
     9781 tatgtttttc gagaaggaac taccgttaat tggttcatta aggcggatga tttagtcttg
     9841 aagcggcagc cgaatcaagg aaatcctacg aaccaaaatg aaagaaagaa ataggtgaag
     9901 attatggaaa ttaatgttga acatatttta gattgtctcg atcaatatgg aaaaggcgag
     9961 ctgactgagg aacaactcgt taaagcatta acctctgatg aaaaaatgtt tctgattata
    10021 catcaaggtt tacttggaga aaatagcgac agtgaagaag actctgatgt actagactgg
    10081 ttaggagaag aagagtcttt ctttatagtt gtcgaagtaa atgaaaactt atgtcggcag
    10141 gcagaatcca ttttggaaga aattggtgtg gagatgccgg atgctattga aggcttttta
    10201 aatcaattgg ttgaaacgaa acaactcccc tcctcgtagc agtaaatgac tagcataact
    10261 ttttgaaatt gcctaatgaa atcctattgg gctttttcga ttggaggtgg aaaccatcaa
    10321 aatgtacaaa ggacatcgca tacgcgcagg agatcagcac ttggtttatc actttgttct
    10381 tggctggcta ctcgctctgt tcatcggttg gatgagcgtc ttttattttc aagagtttag
    10441 acaatttgat atttctaagc tatcactttc tactattgaa atcgtttggt ctataaaaga
    10501 tttagtatgt ctgcttggaa gtctcgcttt ttcaggagct atgatattgc tttatattca
    10561 cttttttctt gatcattggc gaagcttgtg gcatcgacaa aagctagctc ggatgattct
    10621 agagaaccat tggtatgaag tgaaacaaac tcaaagtgaa ggctttttca aagatttgaa
    10681 tagtagccga accagagaga ctatcagtta cttccccaaa atctgttatc gaatgaaaga
    10741 tggattactg tctattcgcg ttcaaatttc acttgggaaa taccaagatc agcttttaaa
    10801 attggaaaag aagttagaaa gcggactgta ttgtgagtta gtagaaaagg aactcaaaga
    10861 ctcctatgtg gagtatactt tactgtatga tatgatcgcc aatcgaattg ggatagacga
    10921 agtagtggca gaaaatggga ctctgcgatt gatgaaaaat caagtatggg cttatgattc
    10981 tttaccccat atgttaatcg ctggtggcac gggtggaggg aagacctatt tcctcctcac
    11041 cattatcgaa gcactattga agtcagatgc agaattgttt atcctcgatc ccaaaaatgc
    11101 agatttagct gatttaggta cggtgatgcc tcatgtttat tcacaaaagg aagaaatttc
    11161 tgcttgtgtg gaagatttct atgaacgtat gatggctcgt agcaaggcaa tgaaagaaat
    11221 gtctaactac aaaacaggag agaattatgc ctatcttgga cttccaccca actttttaat
    11281 ctttgatgaa tacgtggctt atatggagat gttaacgacg aaagaaagtg cggtgatttt
    11341 gaataagctc aaacaaatcg tgatgttggg tcgtcaatct ggtttctttc tgattttggc
    11401 ttgccaaagg ccggatgcga aatatttagg agacggaatc cgcgatcaat tcaactttcg
    11461 cgtggctttg ggtcgtatga gtgaactcgg atattccatg atgtttgggg aggtcgaaaa
    11521 aaacttcttt atgaagcaca tcaaaggtcg aggttatgtg gatacaggtg gcagtgtgat
    11581 tagtgagttt tatacgccac ttgtaccaaa agggtatgat ttcttagatt caattagtaa
    11641 attagaaaag atgaatgatt gagtgatctt caatactgac tttatacttc aagtaacgta
    11701 taatactttt atatagtagg aagaaagggg ataattaata tggaaagtaa tgattcttat
    11761 ttagagtatg caaaagcaaa taaggataag tttatttctt atattatcaa agggaaatcc
    11821 cccgaagttg gacgagacgc agtttttatg gcagggagtc ctggggctgg aaaatcagag
    11881 gttgccattg gattagctga gaattatgat aaccatgtga ttattgatgc ggattacttt
    11941 cgcacccagt ttcctgatta taatggaaaa aactctagtc tctttcaaaa agcatcttct
    12001 tggttggtag agcaggcatt tagatatgtc ttagatcatg gctattcttt tattctggat
    12061 gcaacttttg caatattaag tgctgaaaaa aatatctcgc gggcgttgaa aaaacatttt
    12121 cgagtgacga ttttctatgt ttatcaagac cctaaaatcg cttggaaatt tacacaagaa
    12181 cgtgaaattg cagagggacg tcatgtgccg aaaaaggcat ttattaacgc ttttttcaaa
    12241 tcaagagaga acattgcaaa agtaaagagt aaacatccag atgtattgct tcatatcata
    12301 ataaaagatt atacgaataa tatttccgaa gttcattatg ctacggataa tctagaatta
    12361 tttttaccac tagggtatag taggaaagat ttggaggttc aattacatga ctgagaaaga
    12421 acaagtaacg aaaattgtaa agaaatacaa taagagcatt gcggatttat cggaaaatgc
    12481 tactgcaaaa gagttcaaaa cggtgattaa atatgtagcc gatcaggcca atgaaaaaca
    12541 acggaaatta gttggattaa ataaaaaata gtaggacaaa ttaataatgt atgagccaag
    12601 agcaaaatgt tcttggcttt tttctttgca atgaatgagg taatgtatga gatttttaga
    12661 tttattcgcc ggtattggtg gctttcgttt aggtatggaa caggcgagcc atcattgtat
    12721 cggtttttgt gaaattgata aatttgctag acgtagttat aaagcaattc atgatacaag
    12781 taaggaggtg gaaatgcatg acatcacaag tgtatcagat gagtttattc aatccctcgg
    12841 accagtggac atcctttgcg gcggatttcc gtgccaagct ttttcaattg cgggaaagcg
    12901 gcaaagattt tccgatactc gaggtacttt attctttgaa atcgctcggt tcaccactct
    12961 tctccgacct aagcttttat tccttgagaa cgtccgagga ttgctcaatc acgaaggagg
    13021 ggctacgttc gagacgatcc tccgaacgct ggatggattg gggtatgacg tggagtggta
    13081 ggtgcttaac tcaaaggcct acattcccca aagccgggag cggatattcc ttatcggaca
    13141 ttctcgagac gcatgtaccg aacaagtatt tcctatcatt ggaccgtctt caacatctga
    13201 tcaaaacatc agaaatttgg taaatatcaa tccttccaat cgaggaatgg gtggacaagt
    13261 ctatggttca gatggcgtag cgccaacctt aacaggtgat gaaggaatca aaatcgcact
    13321 gccagcaagg gatggaatct ctgtagcagg actattaccg ggaaagtttg aacaaggtaa
    13381 tcgagtttat gaagttactg gaaccgcccc caccttgtca acgaaacaag gtggtacaaa
    13441 aatcatgatt cgaaaaaatg aaacagaata tcaagaggtg aagccagaag acagtgtgaa
    13501 tctggctttt cctaattcca caagtcgccg aggtcgtctg ggaaaacaga gtgttcatac
    13561 cttactaaca ggagaccaac aagcgatcgt cacggatcag tatcaaattc ggaagctaac
    13621 acctagagag tgttggcgac ttcagggctt tcccgattgg gcttttgatc gagattctca
    13681 agtcaattca gatagtcagt tgtataagca agccggaaac tctgtaacag ttccggtcat
    13741 ttttgatatt gccagaagat taaaagagtt ggatgtacat ggtttatgat gagttgattg
    13801 gagaaattta ttgggtgatt ggaaagattc agtcagaccc agaattagaa gaacaattgc
    13861 gacggttaaa atttgatatt cgcaagaatg gtgtcaaagt cgctggcgat ccatatttaa
    13921 tgaatgagga gacggatgct cgaattgaaa taaatcaagt aattgcagaa tttgagcgga
    13981 ttgcggacca agcaaaagaa ccagatattc gtcaatactt gttagaaatg aaagcagagt
    14041 tggaagtaaa tggtattaca gatgagtaac ctaaaccaac acaactgact gttaggcgct
    14101 gaccgcggag gattcgcgaa gcagaaatcc tccgtggtgc tgtgcctgtt catggacggc
    14161 gttagccgac catgatgtct cacccccgta tctaacaggg gggtacaaaa acacaaaaaa
    14221 taagtaagca accattaaga atgcttgttg tatcaaagat agtaagtaat ttgtataggt
    14281 gttaactcat ttgaaaaagt taacaccttg aatttggagg tgagaacttg gcacaacgga
    14341 atttagatta tcggctatta aaagaccgac gcaacgaata cggcgtttca caaaataaat
    14401 tggctacggc ttgtggactt agtcggccgt acttaaatca gatagaaaat ggtggcgtaa
    14461 ccgcatctat aaaaaccatg aggaaaattt ttgaccaact ggaaagcttt aatcccgatt
    14521 tgcctttgac gttactgttt gactatgtaa ggattcgctt tcccacaacg gatgcacgaa
    14581 aaattattca agaaattctc catttgaaat ttgagtatat gctccatgaa gattatgcct
    14641 tttactccta tcaagaacag tatgtcatgg gtgatattgt ggtgatgttg tctcatgaag
    14701 aagataaagg tgtgctttta gaattaaagg ggcgaggttg ccgacaattt gaaacttttt
    14761 tactcgctca aaagcgtagc tggtacgact ttttcgagga ttgtctaaag gccggtggcg
    14821 tgatgaaacg cttggacttg gcaatcaatg atcgagtggg gctattggat atacctgagc
    14881 taacgaaaaa atgtcagaag gaagaatgta tctccttgtt tcgtaccttt aaaagttatc
    14941 gttccgggga acttttaaag gctgatgaaa aggacggcat gggcaatacc ttatatatcg
    15001 gcagtctcaa gagtgaggtt tatttctgtt tgtatgaaaa agactatgaa caatatatca
    15061 agttagggat tccattagac caaactgaga cgaaaaatcg ttttgagatt cgactgaaaa
    15121 atgaccgtgc ttttcatgcg attcaagatt tgttaaaagg tcgtagcatt gagagcacca
    15181 cgttttccat tatcaatcgt tacttgcgat ttgcggataa agtagaaggt aaaaggcgaa
    15241 ccaactggcc cttgaatgaa caatgggggc gttttattgg acggaatcgg aaagagattc
    15301 aattaacttc cgagccaaaa ccgtatacca ttgaacgaac cttgaattgg ctaggccgac
    15361 aagtcgcccc tacgtggaaa atggctaagg agttggatcg attgaatcgg acaacctata
    15421 tccaggatat ggtacagaat gcacgcttat cagaccgaca taaaaagatt ttggagcaac
    15481 aaagtatggc aattgaaaat ttgattatat gaaaagagga atcgtcatgg gaattgtaaa
    15541 agatattttt ttggtatttg gtggcagtat gcttggtgtt actgtgatgt gcttgatgaa
    15601 tgcaagtgca gcagcggatc gagcaatgga aaagaaagga aagtgacaag atgaatttcg
    15661 gacaaaattt
//
LOCUS       DACDKM010000067        15191 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20834_2016
            SAMN12640370-rid9891663.denovo.067, whole genome shotgun sequence.
ACCESSION   DACDKM010000067 DACDKM010000000
VERSION     DACDKM010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640370
            Sequence Read Archive: SRR10040891
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15191)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15191)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742835.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742835.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:04:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,012
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 29 of 75
            Pseudo Genes (incomplete)         :: 33 of 75
            Pseudo Genes (internal stop)      :: 19 of 75
            Pseudo Genes (multiple problems)  :: 5 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15191
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640370-rid9891663.denovo.067"
                     /isolate="E20834_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            58..1344
                     /gene="efmA"
                     /locus_tag="IV334_001864"
     CDS             58..1344
                     /gene="efmA"
                     /locus_tag="IV334_001864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter EfmA"
                     /protein_id="HAP8943928.1"
                     /translation="MENEQSVVLTNWKRNYLFFLSGQFLSGITSMVVQYAIIWYLTRE
                     TGSATILSFATLLGMIPMVLLSPFVGPLVDRWDKKALLIVTDIIVAIFALILAVVGTI
                     SESFPIWLVFVSLFMRSVAQTFQMPTIQSIMPTIVPSSHITRTNGQLGMVQSANFIIA
                     PALGAALFSVVPVNYLILLDVLGAVFGVGLLIFVKIPKVSPEILEVPLTIFKDAKFGL
                     QQLMDNKGLWYITINGAFVMLLFMPAISLYPLMTLDYFGGSVGQAGAVEVVYAVGMLL
                     GGALISFIGTWKDRMKPIIIAYIIMGLTIGASGLVPNDSQGFLYFLILNAGAGCATPY
                     FNTLLMAMIQQSYESNVLGRVLGNFNSLMNLTGPIGLLFAGPLADRLGVEKMFLFSGI
                     GILLCGIVLFLTSAARNYDKELQKKLVKEHHEQKDE"
     gene            complement(1482..2081)
                     /locus_tag="IV334_001865"
     CDS             complement(1482..2081)
                     /locus_tag="IV334_001865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphatase"
                     /protein_id="HAP8943929.1"
                     /translation="MNYFISDMHFFHERLLGKNDFAPRLFANVDEMNQQLITSWNAVI
                     KETDHVYHLGDLAMHPQYEKANAEILELVTQLQGTIHLIKGNHDSRAFFRYLEKNDPR
                     LTDGSPKFYFYDVGAIVKFDHQQYYLTHYPMLLGPNDTIRNLHGHIHHYSVPIENDVN
                     VGVDAPERELLKEKLPFGTPLSEQQIKEIYEAKRKYDRS"
     gene            2485..3606
                     /gene="mnmA"
                     /locus_tag="IV334_001866"
     CDS             2485..3606
                     /gene="mnmA"
                     /locus_tag="IV334_001866"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HAP8943930.1"
                     /translation="MTDNSKTRVVVGMSGGVDSSVTALLLKEQGYDVIGIFMKNWDDT
                     DENGVCTATEDYKDVAKVAAQIGIPYYSVNFEKEYWDRVFEYFLAEYRAGRTPNPDVM
                     CNKEIKFKAFLDYAMDLGADYVATGHYAQVTRDENGVVHMLRGVDNNKDQTYFLSQLS
                     QEQLSKTMFPLGGMEKSEVRAIAERAGLATAKKKDSTGICFIGEKNFKQFLSNYLPAK
                     KGNMVTLDGEVKGQHAGLMYYTIGQRQGLGIGGGGDSQEPWFVVGKDLATNTLYVGQG
                     FHHPALYATSLDASEIHFTTNEPMPKEFKCTAKFRYRQQDVPVTVRLLDDNRAEVVFD
                     EPVRAITPGQAVVFYDGMECLGGGLIDHAYQETKVLQYV"
     gene            3852..5273
                     /gene="arcD"
                     /locus_tag="IV334_001867"
     CDS             3852..5273
                     /gene="arcD"
                     /locus_tag="IV334_001867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine-ornithine antiporter"
                     /protein_id="HAP8943931.1"
                     /translation="MEKKSTGISKWGLVALVVSSSIGSGVFGITSDLASSAAPGPAIL
                     SWIIVGIGILALVLSLNHLGEKRPDLDGGIFGYAEASFGKLGGFISGWGYWLSAWLGN
                     VAFATMLMSAMGEFFPIFKGGQNVPSILFASIFIWGLTLLVNNGVEGASVINTIVTIC
                     KLVPLFLFLIFVFIAFKVNIFTADFWGNVSDNLVNGTGQQGTIWLQIKGCLMVMMWVF
                     VGIEGASVLANRAKKRSEAQQASIIGLLCLLFIYILASILPYGVLSQEELATISQPAM
                     ANILKGIVGNWGAALINIGLIISIIGSWLSWTMLPAETTMLMARDGLLPKGWGKTNKK
                     NAPTYSLVVTAILTNLFLLTFLVTDYAYQFAYSLCTAAILICYLLVGIYQVQFSLKEG
                     IKKQVVIGGIAVVFELMGIILAGFSYVLLCSIAYLPGFCFYWIACRETKHQITGKEKV
                     MIGLICVAALLSIGLLAVGWIKI"
     gene            5287..6405
                     /locus_tag="IV334_001868"
     CDS             5287..6405
                     /locus_tag="IV334_001868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase"
                     /protein_id="HAP8943932.1"
                     /translation="MKIANFGVEEWLNKWEKKAVYDIAQSSIEALTLDELVGLDGTSV
                     SKFFEKRKNEPLDYGWIEGSDVFKQEVASLYQTVDPENILQTNGATGANLLALYALVE
                     AGDHVVSMLPTYQQLYDIPKSLGAAVDFVHLKEEEDWQFDLEQLEALVKPETKIICLN
                     SANNPTGTLLDRKTLEKIAEIARTVDAYVLIDEVYAPLTDKGEFLSIVDVYEKGIATN
                     SLSKTYSIPGIRIGWTATGPELAEVFRKYRDYTMICGGVLSDDLAVHALKNKEKILAR
                     NKKIITENLVILKQWVANEPKVELVAPNYVSTSFIKLAIEEDDQTFCINLLEETGVLL
                     VPGSAFDLPKHARLGYCCKKETLEKGLFLLSEFLKKQA"
     gene            complement(6465..7757)
                     /locus_tag="IV334_001869"
     CDS             complement(6465..7757)
                     /locus_tag="IV334_001869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HAP8943933.1"
                     /translation="MAARIKEKLFRKRETWEQNLFVLWFGTFMAGIGFSLVMPFMSLY
                     IDTLGNYSTSQLNFWSGLTFSSTFLVTTLISPWWGKLADQKGRKLMLLRASLGMAIVI
                     SLMGCVTSVYQLVALRLLQGIFSGYISNATALVATGTPKEKSGQVLGTLATGSVTGTL
                     LGPLLGGISASAFGYRPTFFITGIILFFVFLLSLFFVHEKFVPVKKENMVSAKQIFKD
                     LKYPHVVIGMFVTTMIIQASNNSISPIISLYIRQLLHGHGNVTLISGIIASIPGIATL
                     IAAPRFGRLGDKIGSERILAIGLGFAILVYIPMAFVTNVWQLAALRFLIGISDACLLP
                     AVQALITKYSPHQAAGRIFSYNQSFQATGNVVGPMIGSSVSSAFGYRGVFLSTSLLVL
                     VNFLLVRHNTQEIHQQETAEEKASGVSIASGHSPSHRQ"
     gene            7907..8467
                     /locus_tag="IV334_001870"
     CDS             7907..8467
                     /locus_tag="IV334_001870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAP8943934.1"
                     /translation="MKKRNLSQEKIIDCFRELAEEMDVQQITFQHLAKRLDIKAPSLY
                     NHFKNIRDVKTALTAKLLQELNDQLRRALVGKSGQEALQIYAETYRSFAFSNQAVYEL
                     LISVPHTNEAVLLDGIHETNQIILQIFEAFQLTKEEKVHRSRELRSMIHGYLSLRFLG
                     YFTKEPTIDPEDSYFWMINNFIATLP"
     gene            8596..8973
                     /locus_tag="IV334_001871"
     CDS             8596..8973
                     /locus_tag="IV334_001871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1033 family protein"
                     /protein_id="HAP8943935.1"
                     /translation="MYQVITMFGDNEPWWFFEDWEEDIEEEETFESFEEARQAYEKQW
                     NEIHQNYEYINAKSNFLSAFWNDGDERWCEECDDDLQQYKGLALLKNHQPITVESRKE
                     FYETTNSSGKTKRCERPKQGAWC"
     gene            8906..10978
                     /locus_tag="IV334_001872"
     CDS             8906..10978
                     /locus_tag="IV334_001872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HAP8943936.1"
                     /translation="MKQLILAEKPSVAKDLSKVLGANQKHKNYYEGPKVIVTWALGHL
                     LGLKMPEDLNKEWQSWQMDTLPMIPKKLGIKPLPKTGHQLKAIKQLAQRKDVSEAVIA
                     TDAGREGELVARWILEYVHFNKPVKRLWISSQTDKAIKTGFKQLRPAKEYDALYESAL
                     ARAKADWLVGLNVTRALTVKYADNLSAGRVQTPTLALVRDQEKKIEQFRPQTYFTITL
                     QAGKEQAKMLQKNQFALKTQEEAQKLVQLISTSKGTVTEVQEKLKTESAPLPYDLTEI
                     QREANQRYGYSAKKTLSLVQSLYETHKIVTYPRTDSKYLTNDMKSTMKERLQAVSDFA
                     PEVKQYLKNGAIVQQTKVFQDSKVTDHHALLPTENRARYEKLSNEERKIYQMIVSRFL
                     GLFAQPHKVSQTKVTVEFGKERFIFRQNRVVQAGWKGETETETETVKWEKGMRINPDF
                     AIKKELSAPPKPLSEASLLGQMEKYSLGTPATRAEIIEKLIKSELMERTPQGLQVSPK
                     GKQLLVLVNPSLVTPDLTESWEKDLEAIAAGKKQAGTFLNGIEKETKRLVNEIKSSKQ
                     EYQDFSITQKKCPECGANLREKNTRDGKIYVCTNSDCNYRRRKDPKVSNHRCPQCHRK
                     MEIIEGKNGAYFRCKYDGTTEKLLDKKEQKKKMTKHEERRLLKKYAQAEEPEESPLAA
                     ALKAAMKK"
     gene            complement(11189..12136)
                     /locus_tag="IV334_001873"
     CDS             complement(11189..12136)
                     /locus_tag="IV334_001873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HAP8943937.1"
                     /translation="MKVSIVVPCFNEEKTVPLFFDEVEKFKGAHEFEYLFIDDGSKDG
                     TLAAIKKLASAYPYVRFVSFSRNFGKEAALYAGLQASTGDLVTVMDVDLQDPPELLPE
                     MINRITTSDIDCVGTRRSTREGEPAIRSFFAKKFYQLINKISDTEMVDGARDFRLMTR
                     QMVDAVLELTEYNRFSKGLFSWVGFKTEYISYENRERVAGETSWSFWKLFNYSIDGIV
                     NFSDAPLTIASFIGALSCVGSGIAILFIILRALLFGDPTSGWPSMVSIFLFIGGIQLL
                     CLGIIGKYIGKIFLETKQRPVYIIRETEKVKPNELKPHQ"
     gene            12382..12846
                     /locus_tag="IV334_001874"
     CDS             12382..12846
                     /locus_tag="IV334_001874"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HAP8943938.1"
                     /translation="MKIHTPIGFLWLDADESGLTAVSFQPISESESANQRILTRTQEQ
                     FEEYFAGKRKEFDVPLSIKRGTPFQQNVWKALSTIPYGEIRTYKEVAVAVDSPKAVRA
                     IGQANRTNPLPIVIPCHRVIGQNGKLTGYMGNAIDGVEIKRQLLLLEGYLQN"
     gene            complement(12908..13951)
                     /locus_tag="IV334_001875"
     CDS             complement(12908..13951)
                     /locus_tag="IV334_001875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative sulfate exporter family transporter"
                     /protein_id="HAP8943939.1"
                     /translation="MKSLSHPIKSVKDSAVREILPGLLLSFAIAAFSRLLAIYLPQLG
                     GATIAILLGILLGNTFFKQPVLAIGTKFAESKLLEFSVFLLGATVTAQTIAQIGVKGI
                     VFVLLQMSVTIIGAYLIGKKLQFSDTSSLMMAGGNAVCGSSAIASIAPSIHAKEEEKG
                     QIITLVNLLGTVMMLTLPFLGLQLFGGNLIEKSALLGGTLQSVGQVVAGASLLDNSVV
                     EFAMLFKILRIMMLVVVVYLFEKFMHKKQPALFETPTDTQKKRKLPLPWYVLGFVIFC
                     LVNSLFSLPVVIDQSAHFISSWFEITALAAIGLRLDFAKFMKEGTRFLSYGLAVGLLQ
                     TVMALLFIWLIKV"
     gene            complement(14328..>15191)
                     /locus_tag="IV334_001876"
     CDS             complement(14328..>15191)
                     /locus_tag="IV334_001876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TDT family transporter"
                     /protein_id="HAP8943940.1"
                     /translation="GNLTGGISMILMIFIFVKLFILFEHTKQTLHDPIIASVSPTFTM
                     SLMVICTYFVSYESIAPIVKFIWLCAVIFQVILVFYFNYHHVIKADLSIEAIYPSWFI
                     IYVGFGVITVTAGNFFPLIGKIFFWISLICYAFLLPIIIKRVFFVRNMAHPTRPLITI
                     VAAPGSLCLTGYLKNFAEPNFYLVVALFALSQVLYFVVLSMLPRLFKLRFYPSYAAFT
                     FPLVISATAIFTTVHYFNSLGIYSSVLDFLKVAECLFASVVVFYVLAHYVRFLIKEHS
                     KHGKFVSKKIA"
BASE COUNT         4975 a         2778 c         3187 g         4251 t
ORIGIN      
        1 cgtataatga ttgttgtgca aacgctatac aaaatgaaaa agaaaagagg gatgtttatg
       61 gaaaatgaac agtcggtcgt tttaacgaat tggaagagaa attatttgtt ctttttatcg
      121 ggacagtttt tgtcaggaat cactagtatg gtcgtccaat atgcgatcat ctggtatttg
      181 acaagagaaa ccggttcggc gacaattttg agttttgcta ctttactagg aatgattccc
      241 atggttttac taagcccatt tgtaggaccg ctagtggatc gctgggataa aaaagctctt
      301 ttgattgtca cggatattat tgtagcgatt ttcgcgctta ttttagcagt agtcggaacg
      361 atttcagaat ccttcccgat ttggctagtt tttgtgtctt tgtttatgcg ttcggtcgca
      421 caaacattcc aaatgccaac gatccagtcg attatgccga caatcgttcc atcttctcac
      481 ataacaagga cgaatggaca gttaggaatg gtccaatcag cgaatttcat cattgcgcca
      541 gctctaggtg cggctttgtt ttcagtcgtt cctgtcaatt accttatttt attagatgta
      601 ttgggggcag tttttggtgt cggcttattg atttttgtga agatcccaaa agtttctcct
      661 gaaatattgg aagttccgct tactattttt aaagatgcaa aatttgggct gcagcaattg
      721 atggataata aagggttatg gtacataacg atcaatggtg catttgtgat gctgttgttc
      781 atgccagcaa tcagtctgta tccattgatg acgctagatt actttggtgg ttccgttgga
      841 caggcgggag cagtggaagt agtctacgca gtcggaatgc tcttaggcgg tgcactgatc
      901 agttttatag gaacatggaa agaccgaatg aaacctatta ttatagccta catcattatg
      961 ggcctgacga tcggtgcaag cggattggtt ccaaacgata gtcaagggtt cttgtacttt
     1021 cttatcttga atgcaggagc aggttgtgca acgccatatt tcaatacact gctgatggca
     1081 atgatccagc agagttatga atcgaatgtt ttaggtcgtg tcttagggaa cttcaactct
     1141 ttgatgaacc tgacaggtcc aattggatta ttatttgcag gaccattagc tgatcgttta
     1201 ggggtagaaa aaatgttctt gttttccgga atcgggattt tattatgcgg aatcgtttta
     1261 tttttaacgt cagctgcccg aaattatgat aaagaattac agaaaaaact ggtaaaagaa
     1321 caccatgagc aaaaagacga atagtatgga atggagttgt gacagaagtc ttgttacaac
     1381 tccatttctg atcctaatgt tcctgtggca ataagcctac tcaaatgaaa aatatgagta
     1441 attatcttta tttgtttaat acttgatata gggcagtttt ttcacgacct atcgtacttc
     1501 ctctttgctt catagatttc tttgatttgt tgctcagata acggagtgcc aaatggtagt
     1561 ttttccttta agagttctct ttccggtgcg tctacgccta cattgacatc gttctcgata
     1621 ggtacgctgt aatgatggat atgtccatga agattgcgga tagtgtcgtt aggcccgagc
     1681 aacatcggat aatgagtcaa gtaatattgt tgatggtcga atttaacgat ggcacctaca
     1741 tcataaaaat aaaatttggg acttccgtca gtaagcctag gatcattttt ttctaaataa
     1801 cggaaaaaag cgcgactgtc atggttccct ttgatcaagt ggattgttcc ttgcagttga
     1861 gtgaccagtt ccaaaatttc tgcatttgct ttttcatatt gcggatgcat agcaaggtcg
     1921 cctagatgat aaacatggtc ggtttctttt attactgcat tccagctagt gatcaactgt
     1981 tggttcattt catccacatt tgcaaagagc cgcggagcaa agtcgttttt tcctaataag
     2041 cgttcatgga aaaagtgcat atcactgata aagtaattca tgtatctttt gcctcccttt
     2101 tttcttagag taaacaaaat cacttatctg agcaactcat aacatctaaa aagaaaaggt
     2161 tgtaaatgag acgtgcagct ttaagcgata agaagcaatt ttgaaaaata gtttttcaca
     2221 atttataaaa ttattcctta ttgccgaaag gctggccctt tttatgagta tgccaatttt
     2281 ttatatcgaa tgctcaaaag atgagtgatt ttttggttga ctatcatgtt tgtgtactga
     2341 ataggtccag tagaacagta tttttacgac ttgataatta agggatgaaa ttttcagaaa
     2401 tatgacgtat aatgtaaaat actgaaaagt tgcgaccttc aactcgtaga tgtttcagaa
     2461 tctaattgaa atgatggtga tatcatgaca gacaacagca aaacacgcgt tgtcgtaggg
     2521 atgagcggcg gtgtcgactc atctgtaaca gcacttttgt taaaggaaca aggatacgac
     2581 gtgatcggaa tattcatgaa aaattgggat gacacggacg aaaacggtgt atgcacggcc
     2641 acggaagatt ataaggatgt agcgaaagta gctgctcaaa tcgggattcc ttattactct
     2701 gtgaattttg aaaaagaata ttgggatcgt gtatttgaat atttcctagc cgaataccgc
     2761 gcaggacgta cacctaaccc agacgtgatg tgcaacaaag aaatcaaatt caaagcattc
     2821 ttggattatg caatggatct tggcgccgat tatgtagcta ctggacatta tgcgcaagtc
     2881 actcgcgatg aaaacggcgt ggtccacatg cttcgtggag tagacaataa taaagatcaa
     2941 acttacttcc ttagccaatt atcacaagaa cagctttcaa aaacgatgtt ccctctaggc
     3001 ggcatggaaa aatcagaagt acgggcaatt gctgaacgcg caggccttgc gactgctaag
     3061 aaaaaagatt caacgggaat ctgctttatt ggtgaaaaga acttcaaaca atttttgagc
     3121 aactatctgc cagcaaaaaa aggaaatatg gtcactcttg atggtgaagt caaaggacaa
     3181 catgctggct tgatgtatta tacgattggt caacgtcaag gactagggat cggtggcgga
     3241 ggagattcgc aagaaccctg gtttgttgta ggaaaagatc tagcgacaaa cacattatat
     3301 gtggggcaag ggttccatca tcctgcattg tatgccacaa gcttggatgc aagtgaaatc
     3361 cactttacga cgaatgaacc aatgccaaaa gaattcaagt gtacagcgaa attccgttat
     3421 cgtcagcaag acgttcctgt gacagttcgc ttattggatg ataatcgtgc agaagtcgta
     3481 ttcgacgaac ctgtacgtgc aatcactcct ggacaagcag ttgtatttta tgatggaatg
     3541 gaatgtctgg gtggtggttt gatcgaccac gcatatcaag aaacaaaagt tttacaatac
     3601 gtttaagcga tattaaatga atcaaaacag ctatggctaa caaaagaaac accttttctt
     3661 ttggatagca tagctgtttt tttgcaataa aattaatttt tattcatttt atacttgaaa
     3721 tacttgtgta aaaagttcat aatatccaat aatatgaata aagttgcttt ttgttaagca
     3781 agagcagttg caaaatagaa gggaatcttt tgcaagtgat ttgttcaatt ttataagtga
     3841 gggtgatgga aatggaaaag aaatcgacgg gtattagcaa atggggactg gttgctctgg
     3901 ttgtcagttc gtcaatcgga agcggtgtgt ttgggatcac aagtgatctg gctagttctg
     3961 cagctccagg gccagcgatc ctttcatgga taatcgttgg gattggtatt ttagctttgg
     4021 tattatcgct gaatcattta ggagaaaagc gtccagatct agatggtggt attttcggtt
     4081 acgcggaagc ttcatttggt aaattaggtg gcttcatcag tggttggggt tactggttgt
     4141 ctgcgtggct gggaaatgtt gcttttgcaa cgatgctgat gagcgccatg ggagaatttt
     4201 tcccgatatt caaaggtgga caaaatgttc cttctatttt atttgcaagt atttttattt
     4261 ggggtctgac tttattagta aacaatggag ttgaaggagc gagcgtcatc aatacaatcg
     4321 tgacgatctg taaattagtg ccattattcc tatttttaat atttgtattt atcgcattta
     4381 aagtgaatat attcactgct gatttttggg gtaacgtatc tgataatcta gtgaatggaa
     4441 cagggcagca aggaacgatc tggttgcaga ttaaaggttg tttgatggtt atgatgtggg
     4501 tctttgtagg gatcgaaggc gcaagtgttt tagccaaccg cgcgaagaaa cgttcagaag
     4561 cccagcaagc atcgatcatt ggtctgcttt gtttattgtt tatctatatc cttgcttcga
     4621 tcttgccata cggcgtctta tctcaagaag agttagcaac tatttcacaa cctgctatgg
     4681 caaatatttt aaaaggaatc gtgggaaatt ggggagcagc gctgatcaat atcggcttga
     4741 ttatttcgat cattggttct tggttgtctt ggacgatgct cccagcagaa acaacgatgt
     4801 tgatggctcg agacggtctg ttgccgaaag gttggggaaa aacgaataaa aaaaatgccc
     4861 caacttattc gttagttgtt actgcaatct tgactaatct attcttattg actttcttag
     4921 ttactgatta tgcctatcaa tttgcctatt ctttatgtac agcggcaatt ttgatttgtt
     4981 atctacttgt cgggatctat caagtccagt tttctttaaa agaaggaatc aaaaaacaag
     5041 tagtgatcgg tgggattgct gtagtttttg aattgatggg catcatttta gcaggatttt
     5101 cttatgtcct tttatgtagt attgcctatc ttcctggttt ttgcttctac tggatcgctt
     5161 gtcgtgaaac caaacatcaa attacgggaa aagaaaaagt aatgattggg ctgatttgtg
     5221 tggccgcact gctttcaatc gggttgcttg ctgtaggctg gatcaagata taagggggaa
     5281 agaaacatga aaatcgcaaa tttcggtgta gaagaatggc taaataaatg ggaaaagaaa
     5341 gcggtatatg atattgcaca aagctcgatc gaagcattga cattagacga actcgttggc
     5401 ttagatggga ctagcgtaag taaatttttc gaaaaacgca aaaacgaacc attggactat
     5461 ggctggatcg aaggatcaga tgttttcaaa caagaagtcg cttctcttta tcaaaccgtc
     5521 gatccggaaa acatcttaca aacgaatgga gcgactggtg ctaatctttt agctctatac
     5581 gcgttagtgg aagcaggaga ccatgttgtt tcgatgcttc ctacttatca gcaattatat
     5641 gacatcccca aatctttagg tgccgctgtt gactttgttc atttgaagga agaagaagat
     5701 tggcagtttg atttagaaca gctagaagcc ttagtcaaac ctgaaacaaa aatcatctgt
     5761 ttgaatagtg caaacaatcc tacaggcaca ttgctagatc ggaaaacttt agaaaaaatc
     5821 gcagaaattg ctcgtacagt agatgcatat gtattgatag atgaagtata tgcgccttta
     5881 acggataaag gtgaattctt gtcgatcgta gatgtctatg aaaaaggtat agcaaccaat
     5941 tccctatcca aaacctactc cattccaggt attcgtattg gctggacagc tactggacct
     6001 gagttggcag aagtttttcg aaagtatcga gactacacga tgatttgcgg cggagtcctg
     6061 tcagatgatc ttgctgtaca tgcattgaag aataaagaaa aaatattggc gcgaaacaaa
     6121 aagatcatta cagagaacct tgtgatttta aaacaatggg tagccaatga accgaaagtt
     6181 gaacttgtcg ctcccaatta cgtctctact tctttcatta aattagccat cgaagaagat
     6241 gaccagacat tttgtatcaa tttattagaa gaaacaggag tactattagt accaggaagt
     6301 gcctttgatt taccgaagca tgcccgacta ggttactgtt gtaaaaaaga aaccttggaa
     6361 aagggattat tcctcttatc tgagttcttg aaaaaacagg cctaaacaaa aacactgcaa
     6421 ggacagtctg tgacaactgt tcaagcagtg tttttcattt acgcttattg tctatgagaa
     6481 ggagagtgcc ctgaagctat ggagactcct gaagcttttt cttctgcagt ttcttgctga
     6541 tggatctctt gtgtattgtg tcgtacaagc aagaagttga ccaaaacaag aagagaagtc
     6601 gaaagaaaga cgccacgata accaaatgca ctggaaacac tggagccgat cataggccct
     6661 actacgttac cagttgcttg gaaagattgg ttataactga agatccgtcc agcagcttga
     6721 tgaggcgaat attttgtgat caatgcttgg acagcaggga gcagacaagc atcagagata
     6781 ccaatcaaaa aacgtaaggc agctaattgc caaacattcg tgacgaatgc cattgggata
     6841 taaaccagaa tcgcaaaacc caaaccaatc gcaagtattc tttcactccc aatcttgtca
     6901 cctaagcgac caaatcttgg agcggctatc aaagtggcaa tcccaggtat cgaagcaatg
     6961 attccgctga tcaacgtaac attcccatgt ccatgcagca attggcgaat gtacagactg
     7021 atgattggac taatggaatt gttggatgct tggatgatca tcgttgtgac gaacataccg
     7081 atgacgacgt gtggatattt caagtctttg aaaatctgtt tggcagaaac catgttttct
     7141 tttttcactg ggacaaattt ttcatggacg aaaaacagac tcaacaagaa gacgaaaaat
     7201 aagatgatcc ctgtaatgaa aaaggtaggg cgatagccaa acgctgaagc agagatcccg
     7261 ccaagaagcg gtccaagtaa tgttcctgta acagaaccag ttgctaaggt tccaagaact
     7321 tgaccacttt tttcttttgg cgtcccagtt gcgacaaggg cagtggcatt actgatataa
     7381 ccagaaaaaa tcccttgaag cagacgcaat gcgactaatt gatacacact agtcacgcaa
     7441 cccatcaaag aaatgacgat tgccattcct aatgaagcac gcaacagcat caattttctt
     7501 cctttttggt cggctaattt tccccaccaa ggagagataa gcgtagtcac caagaaagta
     7561 gatgaaaaag tcaatccgct ccaaaagttc aattgtgacg tactataatt acctaacgta
     7621 tctatgtaaa gtgacataaa aggcatgact aaactaaatc cgataccagc cataaatgtt
     7681 ccaaaccata agacaaacaa gttttgttcc caagtttctc ttttcctaaa taatttttcc
     7741 tttattcttg cagccaatta gactccttct ttctgaaaat tttcatctac agctctttat
     7801 tataaactac aatatgtagt ttatcaatga aagtctcttt tttctctttc tttttcgtgg
     7861 taaactgaaa gagtgaagga tttattttat gagaggaaac atggaaatga aaaaaagaaa
     7921 tctgtctcaa gagaaaatca tcgactgctt tcgagaacta gcagaagaga tggatgttca
     7981 gcaaataacc tttcagcatt tagccaaacg cttggatatc aaggctccct cgctgtataa
     8041 ccatttcaaa aatattcgtg atgtcaaaac agcgctaaca gcgaaacttc tgcaggaact
     8101 taatgaccag ctgcgacggg ctcttgtggg aaaaagtggt caggaagcac tgcagatcta
     8161 tgcagaaact tatcgatcct ttgctttttc aaatcaagca gtgtatgagt tactgatcag
     8221 tgtaccgcat accaatgaag ctgttttatt agatggaatc catgaaacga atcagatcat
     8281 tttgcagata tttgaggctt ttcaattaac gaaagaagaa aaagttcatc gaagcaggga
     8341 acttcgtagt atgatccacg gttatctttc cttacgtttt ttaggatact ttacaaaaga
     8401 accaacaatc gatccagaag acagctattt ttggatgatc aataacttta tcgctacttt
     8461 gccttaaaca atcagattca tttgtaacaa ttttaagatg ttcattaaat gaccctttta
     8521 taagagaaaa ttcttgcaac tttcttgaaa agtgactaag atgagtctag aaaagctgaa
     8581 agaaggagca cgagtatgta tcaagtaatt accatgttcg gggacaatga gccttggtgg
     8641 ttttttgaag actgggaaga agatattgaa gaagaagaaa cattcgagtc tttcgaagaa
     8701 gcacgacaag cgtacgaaaa acaatggaac gaaatccatc aaaattatga atatatcaat
     8761 gcaaaaagta actttctcag cgccttttgg aatgacggag atgagcgctg gtgtgaagag
     8821 tgtgacgatg atttacagca atacaaggga cttgcgttgt tgaaaaatca tcagcccatc
     8881 acagtagaaa gcagaaagga attttatgaa acaactaatt ctagcggaaa aaccaagcgt
     8941 tgcgaaagac ctaagcaagg tgcttggtgc taatcaaaag cacaaaaatt attatgaagg
     9001 acccaaggtg atcgttactt gggcccttgg tcatttatta ggattgaaaa tgccggaaga
     9061 tctgaataag gaatggcaga gctggcaaat ggacacgttg ccaatgatcc ctaaaaaact
     9121 aggaatcaaa ccacttccta aaacaggaca tcaattaaaa gcaatcaagc agctggctca
     9181 gcgaaaagat gtctcagaag cagtcattgc gacagatgcc ggaagagaag gggaacttgt
     9241 tgctcgctgg atcttagagt atgtgcattt caataaacca gtgaagcggt tatggatctc
     9301 ttctcaaaca gataaagcaa tcaaaactgg attcaagcaa ctaagacctg cgaaagaata
     9361 tgatgcattg tatgaatctg ctcttgcccg ggcaaaagcg gactggctgg taggattgaa
     9421 tgtgaccaga gccttaactg tgaagtacgc ggataattta tcagcaggcc gtgtgcagac
     9481 accgacacta gcattagtga gagaccaaga aaagaaaatc gagcagttcc gtccacaaac
     9541 gtatttcacg atcacgttgc aagctggaaa agaacaagcc aaaatgcttc aaaaaaatca
     9601 atttgcttta aaaacacaag aagaagcaca aaaacttgtt caattgatca gtacaagcaa
     9661 aggaactgtc acagaagtcc aagaaaaatt aaaaacggaa tctgcgccat tgccttatga
     9721 tttaacagag atccaaagag aagccaatca acgctatggc tattctgcaa agaaaacatt
     9781 aagtcttgta caaagtttgt atgagaccca taagatcgtt acttatccgc gtacagacag
     9841 caaatatctg acaaatgaca tgaaaagtac gatgaaagaa cgattgcaag cagtcagcga
     9901 ctttgctcct gaggtcaagc agtatttgaa aaacggagcc atcgtccaac agacaaaagt
     9961 attccaagac agtaaagtga ccgatcacca tgcacttttg ccaactgaaa acagagcacg
    10021 ttatgaaaaa ttaagtaatg aagagcgaaa aatctatcaa atgattgtct ctcgtttttt
    10081 aggactgttt gctcaacccc ataaagtcag ccaaacaaaa gttacggttg aatttggtaa
    10141 agaacgattc atttttcggc aaaatcgtgt cgtccaagca ggatggaaag gcgaaactga
    10201 aactgagacg gaaacagtca aatgggaaaa aggaatgaga atcaatcccg atttcgccat
    10261 caaaaaagaa ctatctgcac cgcctaaacc attgtcagaa gcaagcttgc ttggtcaaat
    10321 ggaaaaatac agtctgggaa ccccagctac gcgtgcagag attatcgaga aactaatcaa
    10381 atctgaactg atggaacgta cccctcaagg actgcaagta tctcctaaag gtaagcaatt
    10441 attagtattg gtaaatccct ctttagtaac accagatttg acagaaagct gggaaaaaga
    10501 ccttgaagct attgctgcag gaaagaaaca agcaggaacc ttcttgaatg gaatcgaaaa
    10561 agaaacaaaa cgcttggtga atgagatcaa atcaagcaaa caagaatatc aagatttctc
    10621 gattacccaa aagaaatgcc cagaatgtgg ggccaatctg cgcgagaaaa acacacgtga
    10681 cggcaaaatc tatgtctgca ccaattcaga ttgcaactac cgcagaagaa aagatccaaa
    10741 agtctctaat catcgatgcc cgcaatgtca ccggaaaatg gaaatcatcg aaggtaaaaa
    10801 cggtgcttat ttccgttgta aatacgatgg aacgacagaa aaattgcttg ataaaaaaga
    10861 gcagaagaag aaaatgacca aacatgaaga acgacgcttg ctgaaaaaat atgcacaagc
    10921 agaagaacca gaagaaagcc cattagcagc agcactaaaa gcagcaatga agaagtaata
    10981 agatatgaaa gaggttgtga tagaagtggt cagcttcaag aaataagaag gaatttacga
    11041 aaattgcttc tcaaattttt gtgaaatttc ggcttatttc cgaagaagct acttctggaa
    11101 caccatttat tcggaaaaaa ggactgtgac aagacttttg tcacagtcct ttttcattta
    11161 aaaaaatgtt tacttcattt gacgtatatt attggtgagg cttcaattca tttggcttca
    11221 ctttttctgt ttctcttatg atataaacag gacgctgttt cgtttctaag aagattttcc
    11281 caatatattt cccgatgatt ccaagacata acaattggat tccaccaatg aacaggaaga
    11341 tcgagaccat ggaaggccat cctgatgtag gatcaccaaa caacaatgcc cgcaaaataa
    11401 tgaacaaaat tgcaatacca gaaccgacac aagacaaagc gccgataaat gaagcaatcg
    11461 ttaacggtgc atccgagaag ttcacgatac catcaatcga gtagttgaat aatttccaga
    11521 aggaccaaga tgtttcgcca gccacacgtt cgcggttttc ataagaaata tattctgttt
    11581 taaatccgac ccagctaaat aatccttttg aaaatcggtt gtattctgtt aattctagga
    11641 cggcatccac catttgacga gtcatcaagc ggaaatcacg agcgccatcg accatttctg
    11701 tatcgctgat tttattgatc aactggtaga attttttcgc aaagaaactg cgtatcgcag
    11761 gctcgccttc acgagtcgaa cgccgggtgc cgacacaatc gatatcagaa gtagtgatcc
    11821 ggttgatcat ctcaggaagc agttcgggcg gatcttgcag atcaacatcc atcacggtca
    11881 ctaaatctcc tgtagaagct tgcaatccag cgtacagagc cgcttctttc ccaaaatttc
    11941 tggaaaatga gacaaagcgg acataaggat aagctgaggc tagcttcttg attgctgcta
    12001 acgtaccgtc ttttgatccg tcgtcaataa acaaatattc gaactcatgg gcacctttga
    12061 atttttcaac ctcatcaaaa aaaagaggaa cagttttttc ttcattaaaa catgggacaa
    12121 caattgatac tttcatcgtt tctatccttt caatcgtatg tttatatatg gtttacattc
    12181 aaaatttaat aaaccttaaa taacaccttt aaaatttgat tttacgtaaa agaacaggta
    12241 ttctttttgt aaactaacgt atttagttta ccatgtttcc tcggtttatc aaacttttaa
    12301 gaacattttt tattagttgg caagtagttt tggatagaga aaaaggagga agttccagtc
    12361 tattgactag gagcaaacaa aatgaaaata cacacaccta tcggcttttt atggttagat
    12421 gcagatgaat ccggattgac tgctgtatct tttcagccaa tttcagaaag cgaatcagcg
    12481 aatcagcgaa ttttaacgcg cacacaagaa cagtttgaag aatattttgc gggaaaaaga
    12541 aaagaatttg atgttccttt atctataaag agaggtacgc cttttcagca aaatgtctgg
    12601 aaagctttgt cgaccattcc ttatggagaa atccgaacgt ataaagaagt agccgttgct
    12661 gttgatagtc cgaaagctgt gcgagcgatt gggcaggcaa atcgaaccaa tccattgcca
    12721 atcgttattc cttgtcatcg agtgatcggg caaaatggaa aactgacagg atatatggga
    12781 aacgcaatag atggggttga aatcaaacgc caattgttgt tgttagaagg atatctgcaa
    12841 aattaaaaac agttgtgatt cggacattgt tccatgcgag aatcacaact gttttttcat
    12901 gttcattcta aactttgatc agccaaataa atagtaaagc catcactgtt tggagaagcc
    12961 cgacagctaa tccataagat aagaagcgtg taccttcttt catgaatttt gcaaagtcta
    13021 aacgcagacc aattgccgct aaagcagtga tctcaaacca gctgctgata aaatgtgccg
    13081 attggtcaat aactacaggt aaagaaaaca agctgttgac caaacaaaag atcacaaacc
    13141 ctaatacata ccaaggaagc ggtaattttc gttttttctg cgtatcagtt ggcgtttcaa
    13201 acagagctgg ttgtttcttg tgcatgaatt tttcaaacag atagacaaca acaacgagca
    13261 tcataatacg tagaatctta aatagcatgg cgaattcgac aacagaattg tcaagtaagc
    13321 ttgccccagc gactacttga ccaacagatt ggagtgtacc acccaaaagt gcactttttt
    13381 caatgagatt tccaccaaac aactgtaatc caagaaaagg gagagtcaac atcatcacag
    13441 tacccagtag attgactagt gtgatgatct gtcctttttc ttcctctttc gcatggatgg
    13501 aaggggcgat tgaagcaata gcagaagaac cacaaacagc gtttcctcca gccatcatca
    13561 aagaagaagt atcgctgaat tgcagttttt taccaatcaa gtaagcaccg atgatagtga
    13621 ctgacatttg cagtaacaca aaaacaattc cttttacacc aatttgagca atcgtttgag
    13681 ctgtgacagt tgcacctagt aaaaaaacag agaattctaa tagtttgctt tcagcaaatt
    13741 ttgtgccgat tgctagaaca ggctgtttaa aaaaagtatt tcctaataaa atacccaata
    13801 aaatagcaat agtcgctcca cctaactgtg gcagataaat agcaagaagc ctgctgaagg
    13861 cagcgatggc aaaagaaagc agtaaaccgg gtaagatttc gcggaccgct gaatccttta
    13921 cagattttat tggatgtgac aatgatttca tttgatcggt tcctttcgtc aataaatact
    13981 aaaagcatac tcgaacatag tagcactaaa ccagtatttt gacaaaagaa caagaggtcg
    14041 tgacaaaagt cttgtacgac ctcttgtttc gaataaacgg tgttccagaa gtagcttcta
    14101 cggaaataag ccgaaatttc acaaaaattt gagaagcaat tttcgtaaat tccttcttat
    14161 ttcttgaagc tgaccacttc ttttacgacc tcttggtaaa cggtgttcaa aagcttgcat
    14221 ctgcggtaat aagcccaacc agtacaaaaa catgaacagc tgtttttgtt tggccgttct
    14281 tattacttga tgcagaacag ctttttcaca acctattgaa tcgattatta agcgattttt
    14341 ttagaaacaa attttccatg tttgctatgt tctttaatta agaaacgtac gtaatgagca
    14401 agaacgtaga agacaactac acttgcaaac agacattccg caacttttaa gaaatccaat
    14461 acgctactat agatacctaa actgttgaag taatgaacag tagtaaatat agctgttgca
    14521 gagataacaa gcgggaatgt aaaggctgca tagcttggat aaaaacgaag cttgaacaaa
    14581 cgtggaagca tagacagcac aacgaaatac aatacttgag ataaagcgaa caatgctacg
    14641 acaagataaa aattaggttc agcaaagttt ttaagataac ctgttaggca aagagaacca
    14701 ggtgctgcaa cgatcgtaat cagcggacgt gtcggatgag ccatatttct tacgaaaaag
    14761 acacgtttga tgatgatcgg caataagaac gcatagcaga tcaatgaaat ccaaaagaaa
    14821 atttttccaa taagtgggaa gaagttcccg gcagttacag tgatgacccc aaatccaacg
    14881 taaatgataa accagcttgg ataaattgct tcaattgaaa gatcagcttt gataacatgg
    14941 tgataattga aataaaagac aaggatcact tggaagataa cagcgcacag ccaaatgaat
    15001 ttgacaattg gtgcaatact ttcatatgaa acaaaataag tacaaataac catgagtgac
    15061 atcgtaaaag ttggtgaaac ggaggcgatg attggatcat gcaacgtttg tttcgtgtgt
    15121 tcgaataaga taaacaattt aacaaaaatg aatatcataa gaatcataga gataccgcca
    15181 gttagattgc c
//
LOCUS       DACDKM010000068        15084 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20834_2016
            SAMN12640370-rid9891663.denovo.068, whole genome shotgun sequence.
ACCESSION   DACDKM010000068 DACDKM010000000
VERSION     DACDKM010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640370
            Sequence Read Archive: SRR10040891
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15084)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15084)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742835.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742835.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:04:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,012
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 29 of 75
            Pseudo Genes (incomplete)         :: 33 of 75
            Pseudo Genes (internal stop)      :: 19 of 75
            Pseudo Genes (multiple problems)  :: 5 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15084
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640370-rid9891663.denovo.068"
                     /isolate="E20834_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(167..1246)
                     /locus_tag="IV334_001877"
     CDS             complement(167..1246)
                     /locus_tag="IV334_001877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HAP8943941.1"
                     /translation="MTQTHDNTVSRKGKHLSYSERCQIAILKKEGYSNRQVAKVLGRV
                     PQTINNEIDRGTITQLKRQTQNGKVYDTYYSVYDADAGQAFYEKQRLNCGRRPKWANT
                     NAFIDWADDKMLNEKWSPDVVVGFARKHELFDCAIIPGTTALYQWIDRGIMRTKNMDL
                     LEKLSRKIKAVRPGHRPNKRVLGTSIEERPKEVETRETFGHWEIDTVVGNKTKSDAVL
                     LTLAERQTRFEVILKLNGKDTESVDQAVQQLRERAGANFDQLFKTVTSDNGSEFSGLH
                     DALQDVADVYFSHPYASWERGTSENQHKLIRRFVPKGSPISEVSENQILRIQRWMNDY
                     PRKILGYATPHDTFVQAFKQERLVA"
     gene            1354..2805
                     /locus_tag="IV334_001878"
     CDS             1354..2805
                     /locus_tag="IV334_001878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HAP8943942.1"
                     /translation="MKKSLKLASAVILMSQLLIATPFHVLASENSTYKIAKERTETTE
                     MSNSDVTAESSELLTTSSSESSTTSNAESSTTSSSESSTTSNAESSTTSSSESSTTSN
                     AESSTTSSSESSTISSTESSTTSSSTSEITSSKVTKPNHIESSKKEAPKTEGQTKTFS
                     TPQQIESTESFQSQPILSGNEEIHFDKNESVENFIMKIGESARKIGQKYDLYASVMIA
                     QAILESASGQSQLAQAPNYNLFGIKGTYNGNFVIMVTNEDLGNGTLYTTQSKFRVYEN
                     YEESFEDYAKLLTKGISGNKDFYAGALKANSKTYREATKFLTGRYATDTQYYLKLNEL
                     IKTYDLTNYDKEVKGPQMSKTGYQVPIHNYSITSLFGNRGNEFHRGLDMAATQGEPIH
                     AVKAGKVVKAEFHYSWGNYVVIQHEDGSTALYAHQQQYLVKTGDNVLQGQIIGYVGST
                     GNSTGPHLHLEICKDSSLSQSMLVDPKTLLFGD"
     gene            3154..3786
                     /locus_tag="IV334_001879"
     CDS             3154..3786
                     /locus_tag="IV334_001879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AP2 domain-containing protein"
                     /protein_id="HAP8943943.1"
                     /translation="MKERYGHLTVVEEITHNGYKKYRCLCDCGNETIVYRSNLLSGRT
                     KSCGCGNYKNRHKYKNLTNKTFGKLVAIKPTKLRSEGTVVWECQCLCGRKKLVSAKRL
                     LKGDVKSCGCLKSKKHHIENKKFGLLTAKYPLDNNCSWKTKWVCHCECGSMCEISYSN
                     LFFGHTQSCGCLKRQETRTCVEGTVIECLTSKLPKNNTSGVKGVYRSKEK"
     gene            4374..5813
                     /locus_tag="IV334_001880"
     CDS             4374..5813
                     /locus_tag="IV334_001880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP8943944.1"
                     /translation="MYSYQEDAKVQKNRWWILIAISMFTFMSTLDGSIVNIALPTISD
                     DMGIPMNQAEWVVSVYLMIVCSCLLLFGKIGDSRGKIKVFRIGTLIFVLGSLLCGFNH
                     SLGILLFARVVQAIGASMTMATNAGIITEVFPIHERGRALGAIGAFVSLGSIAGPGLG
                     GLILSQFSWAYIFWINVPVGIVTMLIAEKFLPKDITMSGHTIDGGGFAAIAATIISFF
                     GAIFLGQESGFFHPVPLVLFVISVIAFVSFLKVEKKAASPLISFGIFKNKIFTMSLIT
                     ALLIFSSNFFVNVVIPFYLQNARGLSPSYAGLLMMVFPLMMVVGAPLSGYLTDRVGPE
                     ILVLVGLGLLSVTQVMYMFLSVDSPLWLYIVATAIMGAGNALFQSPNNTMVMSSVTIE
                     NLGLAGSMNSFARNLGMVSGISLSTTVLYQAMSAKMGQQVTNYIANRPDVFLYGMKIT
                     FLGSFVLCFVAFFLTIWRIKKQGGIQKNV"
     gene            6108..6326
                     /locus_tag="IV334_001881"
                     /pseudo
     CDS             6108..6326
                     /locus_tag="IV334_001881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174271.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            6575..6790
                     /locus_tag="IV334_001882"
     CDS             6575..6790
                     /locus_tag="IV334_001882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8943945.1"
                     /translation="MNAGIADMNLIKKTLNDFTSNSISKGTGINLSTIKKLKSGERSV
                     EKLNLLDAIKITEFAMKNGKAEIEIWR"
     gene            complement(7007..7263)
                     /locus_tag="IV334_001883"
                     /pseudo
     CDS             complement(7007..7263)
                     /locus_tag="IV334_001883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287522.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
     gene            complement(7450..8361)
                     /locus_tag="IV334_001884"
     CDS             complement(7450..8361)
                     /locus_tag="IV334_001884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAP8943946.1"
                     /translation="MKIKIERNQKEKSPKKKKERVVSVGKHRKMVLALWLLLSCSLAF
                     GVYKNFTAIDQHTTHEKVVIKEKVVNTSGVESFTEDFAKEYFSWKNNKEAIEKRMSNL
                     EQYLTENGLALIQDMVRADIPTSSEVQSVKILDVEKHSEEFVVSFLVEQHITEGKKTQ
                     SISSAYRVTIFEDENRNHLVTSLPTMIGKPDKAKYEAKQVENDSEIDAKTTEEITDFL
                     ETFFKLYPSASEKELEYYVENGAMRPINIDLKFSELENPVYLQNGKNFLVSTKIKYLD
                     NVSKTTSYFQYKLVLQKNGNWKIVDVK"
     gene            complement(8380..9384)
                     /locus_tag="IV334_001885"
     CDS             complement(8380..9384)
                     /locus_tag="IV334_001885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase P60"
                     /protein_id="HAP8943947.1"
                     /translation="MNLKKVGVISLTFLTISFMGILMCVGLLFGEDSEGNGSSGTSPG
                     GTSVSEEVLKHRAMVEKYAKESDITEYIPILLAIIQVESGGTMEDVMQSSESAGLPPN
                     SLSTEASIKQGCLYFASLVKRSKELGCDQDSIIQAYNYGGGYLDHVGKNGKKHSFALA
                     ESFSKEKSGGQKVDYPNPIAIKENGGWRYNYGNMFYCLLVKQYLTTTQFDDKIVQEIF
                     DEAFKYEGTAYVFGGSSPETGFDCSGLTQWCYAKVDLKLPRVAQDQYDAMTHIDLKDA
                     KPGDLVFFHSTYDAGTYVTHVGIYAGENRMFHAGDPVGWTNLTENYWRQHLIGAGRYK
                     "
     gene            complement(9381..11504)
                     /locus_tag="IV334_001886"
     CDS             complement(9381..11504)
                     /locus_tag="IV334_001886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002407825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HAP8943948.1"
                     /translation="MKKKLFRIVGIVFISILTLIVLLSLVGTVAEATGLVDDTVEAGN
                     LYSKYSLNNYQLDFFVDSSWDWLPWNWGDGLGKSVMYGLYAITNFIWTVSLYLSNATG
                     YVVQEAYKLDFISDTAESIGKNIQTLAGITENGLQTSGFYFGFLLLMILALGVYVAYT
                     GLLKRETTKAVRAVINFVMIFLLSGSFIAYAPTYITKINDFSSDVSEAALTLGTEIVV
                     PNSESQGKDSVDLIRDSLFSIQVQQPWLLLQFDDSNIEEIGEDRVNKILSVSPDENKG
                     KDREEAVKAEIEDNDNANLSITKTMNRLGTVVFLVLFNIGISFFVFLLTGIMLFSQIL
                     FIIFAMFLPISFLLSMLPTYESLGKKAIIRLFNTIMMRAGVTLVITTAFSISTMFFNI
                     SATYPFFMVAFLQIVTFAGIYFKLGDIMSMFNLQSNDSQSMGRRVMRKPQMLMNRKLR
                     QLNRNVGRALAFGGGAAVGNKLAKEQSKSKFKPSGSSLRKNSRLPNDHEILSDLTKEN
                     PVSNNKKQSRMNLAGRKIGKVLDTQALVKDKTKQVKDQVRNTPTNLKYNLHKGIEKSK
                     KAPEEFKRGLFQEKADRVDLREKQRQRRNAKMDEKRKILSEATNRQGKRRANIPIDKA
                     AQPQKDMMLKKEAPKRIVRANLNPEIKRKLTSQEIISKGKSQSASKKNSFQQVKQRST
                     LPKQTNQKVQKRMNHRPKSRSGEKK"
     gene            complement(11509..13956)
                     /locus_tag="IV334_001887"
     CDS             complement(11509..13956)
                     /locus_tag="IV334_001887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HAP8943949.1"
                     /translation="MYPIKYIENNLVFNQEGECFAYYELIPYNYSFLSPEQKYQVHDN
                     FRQLIAQNREGKIHALQIATESSIRATQERSKKEITGRLTEVAKQRIDLQTEALVSMI
                     GDSQIDYRFFIGFKLIATDEEVNLKNLKKSFFSGFQEFVYGVNHHLMGDFVSLSNEEI
                     RRYTKLEKLMESKLARRFKVRRVTPSDLMYLIEHIYGEKGTPFEEYEFQLPKKKLKSE
                     TLVKRYDLLRPSRCLIEEKPRYLCMEHENHESYVAYLTINTIVGEMEFPSSELFYYQQ
                     QQFTFPIDTSMNVEIVTNKKALATVRNKKKELKDLDNHAYQSDNETNSNVLDALDSVD
                     ELETTLDQSKESMYKLSYVVRVSAESVDELKRRCDEVLDFYDDTNVKLVRPFGDMMGL
                     HEEFLPSSKRYMNDYIQYVTSDFLAGLGFGATQMLGELEGIYFGYNVDTGRNVYLKPA
                     LASQGVKDSVTNALAAAFLGSLGGGKSFSNNLLVYYAVLFGGQAVIVDPKGERGGWKE
                     TLPEIADEINILNLTSKPKNQGLLDPYVIMEKKKDSESLAIDILTFLTGISSRDGEKF
                     PVLRKAIRTVTQSNQRGLLQVINELRKEGSPVAENIADHIESFTDYDFAHLLFSDGSI
                     TQSISLDRQLNIIQVADLVLPDAETTFEEYTTMELLSVSMLIVISTFALDFIHSNREI
                     FKIVDLDEAWSFLQVAQGKTLSNKLVRAGRAMNAGVYFVTQNSDDLLDERLKNNIGLK
                     FAFRSTDIHEIKKTLEFFGLDREDESNQKRLRDLENGQCLMQDLYGRVGVVQVHPVFE
                     ELFHAFDTRPPVQERME"
     gene            complement(13943..14332)
                     /locus_tag="IV334_001888"
     CDS             complement(13943..14332)
                     /locus_tag="IV334_001888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAP8943950.1"
                     /translation="MKKIKSYTSIWAVEKVIYAINDFQLPFPVTFNQMAWFVLSLLFV
                     IVFAHVPPLSMIEGAFLKYLGIPVAVTWFMSQKTFDGKKPLGFLRSFISYHLRFKVTF
                     AGKKVKEQKKRWDEPITLVRSVNYVPD"
     gene            complement(14392..14895)
                     /locus_tag="IV334_001889"
     CDS             complement(14392..14895)
                     /locus_tag="IV334_001889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HAP8943951.1"
                     /translation="MEQMRVYIANLGKYNEGELVGAWFTPPVDFDEVKERIGLNDEYE
                     EYAIHDYELPFEIDEYTPIEEINRLCGLAEELEGTPVGEVASEIQHAFFNSFEEMVEH
                     VDDIIYYPDCDDMSDVAYFLIVEAGDFGEVPEHLKTYIDFDACGRDLEIGGNYLVTSR
                     GVFEYPY"
     gene            complement(14908..>15084)
                     /locus_tag="IV334_001890"
     CDS             complement(14908..>15084)
                     /locus_tag="IV334_001890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002570387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8943952.1"
                     /translation="QSLVLLAIVVIGLFLGFKREFSKLIGFLVIALIAVGLVFNASGV
                     KDVLLSLFNRIVGA"
BASE COUNT         4525 a         3032 c         2826 g         4701 t
ORIGIN      
        1 acaggtaact catttgtttg ataccgtaac tgcctcgtta ttctaaatca gtgaggcctt
       61 gataatgaac tgaggtatga tagtcttcag acgattctgg ccccaggcca gctttccaga
      121 catgaagcca tgtcccgagt gaggatcgtt atcaaggaat aatgggtcaa gccaccaacc
      181 gttcttgctt gaatgcctgg acgaatgtat catgtggtgt cgcataaccc aaaattttcc
      241 gcgggtaatc gttcatccaa cgctggatcc gcaaaatctg gttctcgctc acctcactaa
      301 tcgggcttcc cttggggacg aaacggcgaa ttaatttgtg ctggttttcg ctcgtcccac
      361 gctcccaaga agcatagggg tggctgaaat agacgtcagc tacatcttgc agagcatcgt
      421 gtagcccaga aaactccgag ccattgtcgg atgtaacggt cttaaataat tgatcgaagt
      481 tagcgccagc ccgttctctt agctgttgaa ccgcttggtc caccgattcg gtgtccttcc
      541 cgttcaactt taggatgact tcaaagcgtg tctgccgttc agccagcgtc agcagcacgg
      601 catccgactt ggtcttgttt cccactaccg tgtcgatttc ccaatgaccg aacgtttccc
      661 gtgtttcaac ttctttaggg cgttcttcaa ttgacgtccc gagaacacgc ttgttgggac
      721 ggtgtcctgg gcggaccgct ttgattttac gggacagttt ttcaagcaag tccatgttct
      781 ttgttcgcat gattccgcgg tcaatccatt gataaagagc ggttgtgcca ggaatgatgg
      841 cacaatcgaa cagctcgtgt tttctggcaa atccgaccac gacgtccggc gaccattttt
      901 catttaacat tttgtcatct gcccaatcaa tgaaggcatt tgtgttcgcc catttggggc
      961 gtctgccaca gttcagtcgt tgcttttcat agaaggcctg tcctgcatca gcgtcataaa
     1021 cagaatagta ggtgtcatac acttttccat tttgcgtttg gcgttttaac tgggtgatgg
     1081 ttccgcgatc aatctcattg tttatggttt gcggaacacg tcccagcact ttggcaacct
     1141 gacggttgga atagccttcc tttttcagga tagcgatttg gcaacgttct gagtaggaaa
     1201 gatgttttcc cttgcgtgaa actgtgttat catgggtttg cgtcatgtga attcatcctt
     1261 gtctattgag agttgtggta acttcaatat aacatggaat tcacgtggcg ttttttttat
     1321 tttaaatggc taagttcatt ataaaatccg ccaatgaaaa aatctttaaa acttgcatca
     1381 gcagttatat taatgtccca actccttata gcaacacctt tccatgtgtt ggcaagcgaa
     1441 aattcaacat acaaaattgc aaaagaaaga accgagacga ctgaaatgag taattccgat
     1501 gtaacagcag agagtagtga acttttgaca acttcatcaa gtgaaagtag tacgacttca
     1561 aatgccgagt cttcaacaac ttcatcaagt gaaagtagta cgacttcaaa tgccgagtct
     1621 tcaacaactt catcaagtga aagtagtacg acttcaaatg ccgagtcttc aacaacttcg
     1681 tcaagtgaaa gtagtacgat ttcaagtacc gaatcttcaa caacttcaag tagtacaagt
     1741 gaaataactt cttcgaaagt gacaaaacca aaccatatag aatcttcaaa aaaggaagcg
     1801 ccaaaaacgg aaggacaaac aaaaactttt tcgacaccac agcaaataga atcaacagaa
     1861 tctttccaat cacaaccaat actatctggt aatgaagaaa ttcattttga caaaaatgaa
     1921 tctgtagaaa attttattat gaaaattggt gagtctgcga gaaaaattgg tcagaaatat
     1981 gatttatatg cttctgttat gattgcacaa gcgatcttag aatctgccag tggtcaaagc
     2041 cagttagctc aggcaccgaa ttacaattta tttgggataa aaggaactta taatggaaac
     2101 tttgtcatta tggtaacaaa cgaagatttg ggtaatggaa cgctttatac gacccaatca
     2161 aaatttagag tgtatgaaaa ctatgaggaa agctttgaag attatgccaa attattaact
     2221 aagggaatta gtggaaataa agatttttat gcaggagcat taaaagctaa tagtaaaaca
     2281 tatcgagaag caactaaatt ccttacagga cggtatgcaa cagatacaca atactatttg
     2341 aagctaaatg aattaattaa aacctacgat ttaaccaact atgataaaga agttaaaggt
     2401 ccacaaatga gtaagactgg ataccaagtg cctattcata attattcaat aaccagtttg
     2461 tttggaaatc gtggaaatga atttcaccga ggacttgata tggcagctac acagggtgag
     2521 ccgattcatg cagttaaagc tggtaaggtt gttaaagcag agtttcacta ctcttgggga
     2581 aattacgtgg tgatacaaca cgaagatgga tcaaccgcgt tatatgctca ccagcaacaa
     2641 tacctagtaa aaactggaga taatgttttg caaggacaaa taattggcta tgtaggaagt
     2701 actgggaata gtactggccc acaccttcat ttggaaattt gcaaagatag tagtctttct
     2761 cagagcatgt tagttgatcc gaaaacctta ttattcggag actaaaaaag gtaatcaatc
     2821 tgacaaagta acaagaaaat ggttggattc ttagaccttt gatcaggata ttgagactgg
     2881 aattgatatc tcgattacga tgggtttgac aaataaaaca agtccattat cgaatatcga
     2941 gtgtattatt gccgtcttta tgctcatatt ttgagcaggt ttcactagat gaaaacaatt
     3001 tatctatctt gaggatttcg tgtacgtatc gttcagtctt gtattgtaaa ttagtcataa
     3061 aatcagacta agaaaaattg gaaatatttt tttctgattt attaaaagtt aattgaaatt
     3121 atatggtact tctttcttgg gagggtaggt gcagtgaagg aaagatatgg tcaccttact
     3181 gttgttgagg agattactca taatggatat aaaaaatatc gttgcctatg tgactgtggg
     3241 aatgaaacga ttgtatatcg atcaaactta ctatcaggta gaaccaaaag ttgtggatgt
     3301 ggaaattata aaaataggca taaatataaa aatttgacaa ataaaacttt tggtaagtta
     3361 gttgcaatca agccaactaa actgcgaagt gaaggaactg ttgtttggga atgtcaatgt
     3421 ctgtgtggga gaaaaaagtt agtatcggca aagcgacttc taaaaggtga tgtaaaaagc
     3481 tgtggatgtt tgaaatcaaa aaagcatcac attgaaaata aaaaattcgg attacttact
     3541 gcaaagtatc ctttggacaa taactgttca tggaaaacaa aatgggtttg tcattgtgag
     3601 tgtggcagta tgtgtgagat aagttatagt aacttatttt ttggacatac tcaaagttgt
     3661 ggatgtttaa aaaggcaaga aacaaggaca tgtgttgaag ggacagtaat agagtgtcta
     3721 acgtcaaaat taccgaaaaa taatacaagt ggtgttaagg gagtttatcg ttctaaagag
     3781 aaatagattg cgtatataac tttaaaaaaa aagaggtact atttaggatc gtatgattct
     3841 attaagagtg cagctcaagc tagaaaagat gctgaaaata aattgtttca acctttgatt
     3901 gacaaatatc gaggtaatca ttaattattg tggataataa ttaacagtga tatataaaca
     3961 tctataatat attattcata ctattgatat ttagtataat cctatggaat tgaatatcat
     4021 agcaatgtat ttgaaaagca aattggaaaa caaataggtg caacaccata aatatgaaaa
     4081 aattgggaaa aagtagaatg gaaatttgct tacgtctcac gagatagctc acactattaa
     4141 tctgaagaac aatgaagtcg acttttagaa attgctagaa gaaagtaaag cgaattatca
     4201 ctagttacta tagagtaagt tcaaataaag catctccaac ttacatggtt acatgaggat
     4261 atcaaatcga aattactcaa gcttcctatg gctagagtcg cgagaagttg ttgggcagta
     4321 gacataggtt atattgttct tagctacacg atcattagaa aggaaatcct attatgtata
     4381 gttatcaaga agatgcaaaa gtccaaaaaa atcgctggtg gattttgatt gctatatcta
     4441 tgtttacctt catgtccaca ttggatggca gtattgtcaa tattgcacta cctaccatct
     4501 cagatgatat gggcatacca atgaatcaag cggaatgggt agtgtccgtt tatctgatga
     4561 ttgtctgttc ttgtttgttg ctgtttggta aaattggcga tagccgtggg aaaatcaaag
     4621 tatttcggat tggtacgctg atctttgtct tgggatcgct gttgtgtggg ttcaatcatt
     4681 cgctaggtat tttattgttc gcacgggtgg tacaagccat tggtgctagt atgactatgg
     4741 ctacaaatgc cggaatcatc acggaagtct ttcccataca tgaacgtggt cgggcattgg
     4801 gagcaatagg tgcgtttgtt tcattaggct cgattgctgg accaggtctt ggcggcttga
     4861 ttttgtccca attttcttgg gcgtacattt tttggatcaa tgtaccggtg ggaatcgtaa
     4921 cgatgttgat tgctgaaaaa ttcttaccaa aagatatcac catgagcggc catacaatcg
     4981 atggaggggg ctttgctgcc attgcggcta cgattatcag cttttttgga gcaatctttt
     5041 tggggcaaga aagtggcttt ttccatccag tcccccttgt actgttcgta atttcagtga
     5101 ttgcctttgt cagcttttta aaagttgaaa agaaagcagc ctctccattg atttctttcg
     5161 gtatttttaa gaacaagatt tttaccatga gcttgattac agccttgttg attttttctt
     5221 ccaacttctt tgtcaatgta gtgattcctt tttatctgca aaatgctcgc gggttgagtc
     5281 ctagctatgc tgggctgttg atgatggtct ttcctttgat gatggtcgtc ggggcgcctt
     5341 taagcggcta cctgaccgac cgggttggcc cggagatttt ggtattagta ggcttgggct
     5401 tattgtcggt aacccaggtc atgtatatgt ttttgtcagt ggactctcct ttgtggctat
     5461 acattgtcgc tactgccatc atgggtgccg gcaatgcctt gttccagtcc cccaataata
     5521 cgatggttat gagctcggtc actatcgaaa acttgggact ggcaggctcg atgaattcct
     5581 ttgcccgaaa tttggggatg gtcagcggga tttcgctttc tacgacggtc ttatatcaag
     5641 cgatgtctgc taagatgggc cagcaagtaa ctaactatat cgcaaatcgg ccggatgtct
     5701 ttttatatgg aatgaagatt acctttttag gcagctttgt tttgtgtttt gttgcctttt
     5761 tcctgactat ctggcgtatc aaaaaacaag gaggtattca gaaaaatgtc tgaaaaaacc
     5821 actgttgaag aatttttgga ggcctgcgta tgggtgagtg ggatttccat ggatgatgga
     5881 agatgaagtg aacttctaaa agccgaaaaa ctcaagtatt atcttcaaaa tactagtgct
     5941 caatgagatg cttgatttga tcacaagaag actgcgaaag ccgctggcaa ccgcaagcta
     6001 ggtatcccac cgatggacta cgttttagag gctactggtt atgaatacgg agccaacgct
     6061 ctcatctgca taagaataca tcacccagat tatgtaaaga ttttcgatac ttctgtcaaa
     6121 aaatcattaa gatagataaa tgatttccgt ctagtcaaat tggttcagaa tgtagacaca
     6181 cagatggtaa gaagtcactc acaattcgag aatggacttg tcctatttgc cgtatacatc
     6241 ataatcgaaa cgtcaacgat agtatcaata ttttgaccaa gggctatgaa tacaagcatt
     6301 gccttagaaa acatcaagaa ccttaggaac aacggggata gcttggttaa taagagatac
     6361 cgctgtttag gcttgcaagt atactctttt atcaagaatt ccgtggcttt agtcatgaga
     6421 ggcttaatac tatgaaaatt ttctttagca tttttgattt aggttccttt tctaattata
     6481 ataagatgac cactacagta acctactgaa atatataaac agccactgta gtaccatttt
     6541 caaagtagtc tatgagagga gaatcttcat tcttatgaac gctggaattg cggatatgaa
     6601 tttaataaaa aaaaccctta atgattttac ttccaattca atatcaaaag gtactggtat
     6661 caatttaagc acaataaaaa agctgaaaag tggtgaaaga tcagtggaaa agctgaatct
     6721 tttagatgct ataaaaataa cggaatttgc tatgaaaaat ggaaaagctg agattgaaat
     6781 atggagataa ttatcgtcta gtgaattact tgccacttag ctgaatttaa cgatccttgg
     6841 tgcttgaagg gggagattct tgggaataga gcatacttgc aagcctatgt ctttactaac
     6901 aacgatatct cttattaacc aagctatccc cgtagttcta aggcttttac ttttatctaa
     6961 gcaataatcg tattcaaagg aaatttttga cccttagaaa gtctcatttt atcagacgtg
     7021 agcttaggca ttgctgaatt ttgttaattc ggttttaggt tgtgctttga gcataatatg
     7081 aaagtattct ttatcacgat ttcattcatc taaggtaatg tgatgtgatt ctgaaattct
     7141 ttcaaaggca gtttttgcat aagctgacat tacatcatca atgacatgtt tacaatgttt
     7201 cactactaaa ataagggggt aaccaagaag aaaaactgaa tgattatttg tatctaattc
     7261 catggtatat caaccatttc ctatatagac tgattatgac atggattaaa ataaaaaggc
     7321 actatgtctt aattgtcggt tttcgagtta ccgaatggca ttcatcaccc acctatagag
     7381 gatgggcgac ttctgcctga aataagttac actacttata acttctaata aaattagatt
     7441 gagtcattat tatttaacat caacgatttt ccagtttcca tttttttgta atactaattt
     7501 gtactgaaag tagctagtgg tttttgatac attatcaagg tattttatct ttgtactcac
     7561 tagaaaattt tttccgtttt gtagataaac tggattctct aactccgaaa actttaagtc
     7621 aatattgatt ggtcgcatcg caccattttc aacataatac tccaattctt tttcagaagc
     7681 tgatggataa agtttaaaaa atgtttctaa aaaatcggtg atttcttccg ttgttttggc
     7741 atcaatttct gaatcgtttt ctacttgttt tgcttcatat tttgccttgt ctggctttcc
     7801 aatcattgtt ggcaaactag ttactaaatg attcctgttt tcatcttcaa agattgtcac
     7861 tcgatatgca gaagaaattg attgcgtctt ttttccctct gtaatatgtt gctccactaa
     7921 aaatgaaacg acaaattctt ccgagtgttt ttcaacatct agaattttta cagattgaac
     7981 ttcggaactc gtgggaatat ctgctcgaac catatcttgg attaaagcga gaccgttttc
     8041 tgtaaggtac tgttcgagat tactcattct cttttcaatt gcttctttgt tatttttcca
     8101 tgaaaaatat tctttcgcaa aatcctctgt aaaactttca acgccagaag tattcactac
     8161 cttctctttt ataaccactt tttcatgagt tgtatgttga tcaatggcgg tgaaattttt
     8221 ataaacgcca aaagcaagac tacaagacaa taaaagccaa agggcaagca ccatttttcg
     8281 atgcttaccc acggatacaa ctcgttcttt tttctttttt gggctctttt ccttttgatt
     8341 ccgttcaatt tttatcttca ttctttttcc tcttttctct tatttgtatc ggccagctcc
     8401 gataagatgc tgccgccaat agttttccgt tagattcgtc caccccactg gatctcctgc
     8461 atgaaacatg cgattctccc ctgcgtaaat gcccacatgg gtcacataag taccggcatc
     8521 ataggttgaa tgaaagaaaa ctaaatcacc tggtttggca tcttttagat caatatgagt
     8581 catggcatca tattgatcct gagccacccg aggaagtttc aggtcaactt tggcataaca
     8641 ccattgggtc aagccactac aatcaaaccc cgtttctgga cttgagccac caaacacata
     8701 ggccgttcct tcatacttaa atgcctcatc aaaaatttct tggactattt tgtcatcaaa
     8761 ctgagttgta gttaggtatt gcttcactaa taaacagtaa aacatattgc cataattata
     8821 ccgccaccca ccattttcct taatcgcgat tgggttgggg taatcgactt tttgaccacc
     8881 tgatttttcc tttgaaaaag attcagctaa agcaaaacta tgcttcttcc cgtttttccc
     8941 gacatgatct agatacccgc caccatagtt ataggcctga ataatactat cttgatcaca
     9001 ccctagttct ttggaacgct taaccaaaga cgcaaaatag agacagcctt gtttgatgga
     9061 tgcctcggta cttaatgaat taggtggtaa tccggctgac tcagaacttt gcataacgtc
     9121 ttccattgtg ccaccagatt ccacttgaat aatcgccaaa agaattggaa tatactctgt
     9181 tatatccgat tctttggcat acttttccac cattgcacgg tgtttcaata cttcttctga
     9241 gacactagtt ccgccaggag aagtgccaga actaccattt ccctcactgt cttcaccaaa
     9301 caacaaaccc acacacatca aaatacccat aaaagagatg gtcagaaaag ttaatgaaat
     9361 aacacccact ttcttcaagt tcattttttc tcacctgacc ttgattttgg acgatggttc
     9421 attctttttt gtactttttg attggtctgc tttggcagag tggacctctg tttcacttgt
     9481 tggaaagaat ttttcttact tgctgattgg cttttccctt ttgaaatgat ttcttgacta
     9541 gttagttttc gtttgatttc gggattcaga tttgctctca caatccgctt tggtgcttct
     9601 tttttcagca tcatatcttt ctgaggttga gctgctttat ctattggaat attcgccctt
     9661 ctttttccct gacgatttgt tgcttcactc agtatcttgc gtttttcgtc cattttcgca
     9721 tttcttcgtt gtcgttgttt ttctcgcaaa tccactcgat ccgctttttc ttggaacagt
     9781 ccgcgcttga attcttctgg tgcttttttc gacttttcga ttcctttatg gagattgtat
     9841 ttcaaattcg ttggcgtatt ccttacctga tcctttactt gtttagtctt atctttcacc
     9901 aaagcttggg tatccaaaac tttgccgatt tttctcccag ccaaattcat tcgggattgt
     9961 ttcttattat ttgaaactgg attctcctta gttaagtcag atagtatttc gtgatcgttc
    10021 ggtaatcgtg aattctttcg tagagaagaa ccggatggct taaatttgga ttttgattgt
    10081 tcctttgcca acttatttcc gactgctgca ccaccaccaa aagccaaagc tcgtcccaca
    10141 tttcgattga gttgccgcaa ttttcgattc attaacattt gcggttttcg catgacgcgt
    10201 cttcccattg actggctatc attactctgg aggttgaaca tactcataat gtcgcccaat
    10261 ttgaaataga taccggcaaa ggtcacaatt tgcagaaaag caaccatgaa aaatggatag
    10321 gttgccgaaa tattgaaaaa catcgtggag atgctaaatg cagtcgtaat caccaaagta
    10381 acacccgctc gcatcataat cgtattgaat aaacgaataa tcgccttctt tcctaaactt
    10441 tcataagtcg gcaacatgga caataagaaa ctaattggca gaaacatcgc aaagataatg
    10501 aagagaattt gcgagaacaa cataatccct gttagaagaa aaacaaagaa ggaaatgcca
    10561 atattgaaca ataccaaaaa gaccacggtc cctaaacggt tcatggtctt ggtaatactg
    10621 agattcgcat tgtcattgtc ttcaatctct gccttgaccg cttcttcccg atctttgcct
    10681 ttgttttcat ctggactcac agatagaatc ttgttgaccc ggtcttcacc aatttcttct
    10741 atgttggaat catcaaattg cagtagtaac caaggttgtt ggacttgaat cgaaaacaaa
    10801 ctatcccgaa tcaaatccac actatctttt ccttggcttt ctgaattggg tacaacgatc
    10861 tcggttccaa gggtaagggc cgcttcacta acatctgagc taaagtcatt gatttttgtg
    10921 atataggtag gcgcataagc aatgaacgaa cccgatagta gaaaaatcat cacaaaatta
    10981 atcacggctc gaactgcttt cgtggtttct cgttttaata atccggtata cgccacatac
    11041 acccccagag ctaaaatcat caataataga aacccaaaat aaaatccgga ggtctggagt
    11101 ccattttcag taataccagc caatgtttgg atattctttc caatactttc agccgtatcc
    11161 gaaatgaaat ctaatttgta agcctcttga accacataac ccgtggcgtt cgacaaatac
    11221 aaactcaccg tccaaatgaa attagtaatg gcataaagac catacatcac gcttttccct
    11281 aagccatcac cccaattcca aggcaaccaa tcccaagagc tatccacaaa gaaatctagt
    11341 tgatagttgt tcagtgaata tttggaatat agatttcctg cttcaactgt atcatcaact
    11401 agccctgtcg cctcggctac cgtaccaact aaactcaata gaactatcag agtgagtatg
    11461 ctaataaaaa cgattcccac aatccgaaaa agtttctttt tcatcccctc actccattct
    11521 ctcttgaaca ggtggacggg tatcaaaggc atggaagagt tcttcaaaga ctggatgtac
    11581 ctgaaccacg cctacccgac catacaaatc ctgcatcaag cattggccat tttccaaatc
    11641 tcgtaggcgt ttttgattgc tttcatcttc tcgatccaag ccaaagaatt ccaaggtttt
    11701 cttgatttca tgaatatcgg tactgcgaaa cgcaaacttc aacccgatat tattcttcaa
    11761 gcgttcatcc agtaaatcat cactattttg cgtgacaaag taaaccccgg cattcatagc
    11821 ccgcccagca cgaacgagct tattcgataa tgttttccct tgcgctactt gaagaaaact
    11881 ccacgcctca tctaagtcta caattttgaa gatttctcga ttactatgga taaagtccaa
    11941 ggcaaaagtg gaaatgacga ttagcatgga aacactaagc aattccatgg tggtatattc
    12001 ttcaaaagtg gtttctgcat ctggtaaaac caaatccgct acttgaatga tatttaactg
    12061 cctatctaaa ctaatcgact gggtaatcga accatctgag aaaagcaaat gggcaaaatc
    12121 ataatcggta aaggactcga tgtgatccgc gatattttcc gcaactggtg agccttcttt
    12181 tcgaagctca ttaattactt gtaataatcc ccgttgattg ctttgggtaa ccgtccgaat
    12241 ggccttacgc aacactggaa acttctcccc atctcgacta gaaattcctg ttaaaaaggt
    12301 cagaatatca attgctagac tttcagaatc cttctttttc tccataatca cataaggatc
    12361 aagtaatcct tgattttttg gcttactagt taagttgaga atattgattt catccgcaat
    12421 ttctggcaag gtttctttcc agccaccccg ttcccctttt ggatcgacaa tcacagcttg
    12481 tccaccaaaa aggacggcgt aataaactaa gagattgtta ctaaaggact ttccgccacc
    12541 gagtgaaccg aggaatgccg ctgctagcgc attggtaact gaatccttga ccccttgtga
    12601 ggccaatgcc ggtttcaggt aaacattgcg ccccgtatct acgttataac caaagtaaat
    12661 gccttccaac tcccctaaca tctgagtggc gccaaaacca agtccggcga gaaaatcaga
    12721 cgtaacatac tggatataat cattcatgta tcgcttacta gaaggtaaga attcttcatg
    12781 tagccccatc atatcgccaa aaggccggac tagttttaca ttggtatcat cataaaaatc
    12841 tagcacttca tcacaccggc gctttagttc atctactgat tcggcactta ctcgaaccac
    12901 atagctcagt ttgtacatgg attctttgga ctgatccaag gtagtttcca attcatctac
    12961 ggaatccaat gcatctaaga cattggaatt tgtttcattg tcagattgat aggcatggtt
    13021 gtccaagtct tttaattcct ttttcttatt gcgaacggta gcaagtgcct ttttgttcgt
    13081 cacaatctcc acattcatac tagtgtcaat tgggaatgta aattgttgtt gctggtaata
    13141 gaaaagttct gaggagggaa actccatttc ccccacaatt gtattaattg ttaagtacgc
    13201 tacatatgat tcatgattct catgttccat gcataagtac cggggctttt cttcaatcaa
    13261 acaacgactg ggacgtaata agtcgtaacg tttcaccaaa gtttccgact tcagcttttt
    13321 ctttggtagc tgaaattcat attcttcaaa tggtgtaccc ttttcgccat agatatgttc
    13381 aatcaaatac atcagatcac tcggtgtgac tcgtctgact ttaaatcttc gagccaattt
    13441 actttccatt agtttttcaa gcttggtata tcggcgaatt tcttcattgg ataaggaaac
    13501 aaaatcgccc atcaaatgat gattcactcc atagacaaat tcttgaaagc cggagaagaa
    13561 agatttcttg agattcttta gattgacctc ttcatctgtc gcaatcagtt tgaagccaat
    13621 aaaaaagcga taatcaatct gactatcgcc aatcatggat actaatgcct ctgtctgtaa
    13681 atcaattcgt tgtttcgcta cttctgttaa tcgcccggta atttcctttt ttgatcgttc
    13741 ttgcgtggca cgaatactac tttctgtggc aatttgtaag gcatgaatct tgccttcacg
    13801 attttgcgca atcaattgac gaaagttatc gtggacttga tatttctgtt caggtgacaa
    13861 aaaggagtaa ttgtaaggta ttaactcata gtaggcaaaa cattctccct cttgattaaa
    13921 gacaagatta ttttcaatat acttaatcgg gtacatagtt cacactcctt accagagtaa
    13981 tgggttcgtc ccatcgtttc ttctgttctt tgactttctt tcctgcgaaa gtgaccttaa
    14041 atcgtaagtg atagctaata aaagatcgta aaaatcccaa tggtttctta ccgtcaaacg
    14101 ttttttgcga catgaaccaa gtaactgcca ccggtatacc taagtatttt aaaaaggccc
    14161 cctctatcat agatagtgga ggcacatggg caaatacgat gacaaacagc aaggataaaa
    14221 caaaccaagc catctggtta aaagtcacag gaaaaggcag ttgaaagtca ttaattgcat
    14281 aaatgacctt ttcaactgcc caaatactgg tgtagctttt tattttcttc atcaaatcat
    14341 tcctttaaaa agaaggatag tttagaaatc aactaccctt caataatttt gttaatatgg
    14401 atactcaaaa acacctcgac tcgttaccaa ataattgcct ccaatctcca agtctctacc
    14461 acacgcatca aaatcaatat acgtcttcaa gtgttctggt acttctccaa aatcaccagc
    14521 ttctacaatc aagaaatatg ctacatcact catatcatcg caatctggat aatagataat
    14581 atcatccaca tgctctacca tctcctcaaa agaattgaag aaagcatgtt gaatctcaga
    14641 agcaacttca ccaactggcg ttccttcaag ttcctccgct aagccacaca accgattaat
    14701 ttcctcaatt ggcgtatact catcaatctc aaagggaagt tcataatcat gaattgcata
    14761 ttcctcgtat tcgtcattta acccaattcg ttctttcact tcatcaaaat cgacaggtgg
    14821 ggtaaaccac gcccccacca attcaccttc gttatatttc ccgaggttcg caatataaac
    14881 acgcatctgt tccatcgtgc ttccccctta cgcaccgaca attcggttaa acaaactgag
    14941 taagacatcc ttcaccccag aagcattaaa gaccaatccg accgcaatca aagcaatcac
    15001 caaaaagcca atcagtttgg aaaactcccg cttaaatcct aaaaacaaac caatgaccac
    15061 aattgccagt aagactaagg attg
//
LOCUS       DACDKM010000069        14446 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20834_2016
            SAMN12640370-rid9891663.denovo.069, whole genome shotgun sequence.
ACCESSION   DACDKM010000069 DACDKM010000000
VERSION     DACDKM010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640370
            Sequence Read Archive: SRR10040891
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14446)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14446)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742835.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742835.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:04:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,012
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 29 of 75
            Pseudo Genes (incomplete)         :: 33 of 75
            Pseudo Genes (internal stop)      :: 19 of 75
            Pseudo Genes (multiple problems)  :: 5 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14446
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640370-rid9891663.denovo.069"
                     /isolate="E20834_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(143..1345)
                     /locus_tag="IV334_001891"
     CDS             complement(143..1345)
                     /locus_tag="IV334_001891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAP8943953.1"
                     /translation="MKTKYPCVYQDKNGRFSYLVELGTDQVTGKRIQKKGTKDSLGKR
                     FSSAREAHKEVMRIKNEYMVVNGFANYDLTYKEFMEQSYVPHYKASVQKSTWSSRECG
                     LIQITEFFGDKKLREINVRDCENYRIWLLNDSGYSQAYSSLMYGMFRKSLDYAVTLQF
                     LNENISKRTKAIPKGKSIVPYWTKEEFEEVLAKIYKDDFYEHMCFLMIWLYYMTGVRV
                     SEGLALNWNDVDFKRKKLRVHHTLEMTNQKDFIRKPYTKTESGLRIISLDDDTIEVLK
                     KWKVIQKNHGVEQFILSYTDLPLYRSTVQRIIQRYAKLAKVPLIQGKGLRHSHVSYLI
                     NEFNADILVVSQRLGHSSPEITLKHYAHLWSRNDEGIAEQMTGNIKFTCATKSKVANF
                     NGNQAVKI"
     gene            complement(1348..1542)
                     /locus_tag="IV334_001892"
     CDS             complement(1348..1542)
                     /locus_tag="IV334_001892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 domain-containing protein"
                     /protein_id="HAP8943954.1"
                     /translation="MKNTICRKDLEALGFKTHQATTIIRQAKTRMVNQGYPYYNNKRV
                     GVVPRQVIEEIIGVPLSQEE"
     gene            complement(1589..2437)
                     /locus_tag="IV334_001893"
     CDS             complement(1589..2437)
                     /locus_tag="IV334_001893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rep protein"
                     /protein_id="HAP8943955.1"
                     /translation="MQAYNQKIIETPTYIEIYDYEAIITPKRKTIENEVNLERKQKLA
                     WIYENDITLEKRKNYDELSAQGQYDSLKRKQKHYQSMRFEIARLVDTNFDNQTKFLTL
                     TFKENIQDIEYANNEFKKFIKRLNYDLYKTKKANLKYLATWEKQQRGAIHYHIILFDF
                     PFISFNRLTTIWGQGFVKINKIDVDSIENRGRYISKYFDKELELKEHKKKAFFKSRNL
                     KLPTVTKKMVKKPYDSTNQDILYTSEYIVRRPFFYNALALNELGSPEQVMDFQESTVH
                     YTKIKK"
     gene            complement(2642..2992)
                     /locus_tag="IV334_001894"
     CDS             complement(2642..2992)
                     /locus_tag="IV334_001894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002401157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8943956.1"
                     /translation="MPIKITDLSLPKETLEKEYQVVNVTRWQKEGEILGWSYECILPK
                     LRYEKISVKVKSEHPVITPEELTQQGLAIITFTNLVIVPWGRSNGQFVSYGLSATADT
                     ATLIPRKQGTTTQN"
     gene            3587..4033
                     /locus_tag="IV334_001895"
     CDS             3587..4033
                     /locus_tag="IV334_001895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cro/Cl family transcriptional regulator"
                     /protein_id="HAP8943957.1"
                     /translation="MKDYVVQITKEVLEESKTLNKLSSKAKEEAVTILSSGIIDYIEM
                     SGEIKYGEKDRIAKMVKESIKTFDVKQDLTFNDSYLIGKLINILGTDEDTELFLSSLS
                     MIFTDKHFSTRFGGEELVEIFQSMRPALIKLYREKTPDEFYDWFEK"
     gene            4657..5649
                     /locus_tag="IV334_001896"
     CDS             4657..5649
                     /locus_tag="IV334_001896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010748893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP8943958.1"
                     /translation="MYWEEIELEKTLGPGGEIRAVQRDKKNISKWEKLKKQLRRDKWM
                     YLLLLPGIIMTFIFKYVPMYGAVIAFKDYNPMLGILESPWVGLDHFREFISSPNFTLL
                     LSNTLKISIYGLLLGFFPPIILALSLNQIINKKIKQKIQLILYAPNFISVVIIVGMLF
                     LFLSANGPINGLVEKVTGQPIMFMSNPAYFRTIYILSGIWQGIGWSSILYTATLAGVS
                     TDLYEAANIDGANILQKIWHIDLPALRPVMVISFILAAGGIMNVGYEKAYLMQTSMNI
                     PASEIISTYVYKVGLQSGDYAYSAAVGLFNMIINVVLLLIVNNITKRLNDGQGL"
     gene            5665..6549
                     /locus_tag="IV334_001897"
     CDS             5665..6549
                     /locus_tag="IV334_001897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018165023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAP8943959.1"
                     /translation="MNSALSKIDRQILWLNRVIVTFLVVITIVPLIYVLVASFMDPAT
                     LINKGISFNPKDWTIQGYQRVFADDSILRGFVNSLFYSFSFSLLTVFITMITAYPLSR
                     PNLAGKKIIMTFFVITMFVGGGLIPTYLLVKNLGMLDSVWAIIVPGAINVWNIILART
                     YFSALPKELSEAATIDGANDLQILFKVMLPLAKPIMFVLFLYAFVGQWNSYFDAMIYI
                     KDPDLQPLQLVLRNILIQNQPSQDMVGANTAMAEMKQIAELIKYATIVISSLPLIIMY
                     PFFQKYFDKGVLVGSVKG"
     gene            6598..8205
                     /locus_tag="IV334_001898"
     CDS             6598..8205
                     /locus_tag="IV334_001898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HAP8943960.1"
                     /translation="MKAKWMLPIVFTSVLILGACGGSKEKSKSSPDYELENVSFPLKE
                     KVSLKMMSQSAPLAPNDPNDKLIFQRLEEETNLHIDWTNYSSDFAEKRNLDIASGDLP
                     DAIFNAAAGDYDLLNWAESGVIVPIEDLIEDYMPNLKKIFDENPEYRQMSTAPDGHIY
                     SMPWIEELGEGKESIHTVNGMAWINKEWLDNLGLEMPQTTDQLMTVLEAFKTQDPNGN
                     GEADEIPFSFINNGGNEDLKFLMGAFGLGDNDDHLVVDNDGQVQFTANKEGYKEAIKY
                     FNEMNNKGLIDKEAFEQDWNTYVAKGKEHKYGVYFTWDKANITGADDSYDVLPVLAGP
                     DGTKHVTRTNGMGFSRDRFVITSANKNLELTARWIDKMYEPLQSVQNNWGTYGDEAQA
                     NIFELKDGMLKHLPLEGTAPGELRQKTEAGGPLAVLDSYYGTVTTMPDDAKWRLDLMH
                     DTYLPYITEDNIYPRVFLEKEETDRLAKIETDMWDYIYRKRAEWITGGKIDEEWEEYL
                     AELERVGLEEWIQIKQTGYDNFLKGAQ"
     gene            8206..9690
                     /locus_tag="IV334_001899"
     CDS             8206..9690
                     /locus_tag="IV334_001899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016621147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 32 protein"
                     /protein_id="HAP8943961.1"
                     /translation="MLVEETAILDKANRFIEMMRGKVNPQYRQNYHFSAPIGWINDPN
                     GFIYYKGHYHLFYQYYPYDSRWGPMHWGHARSSDLVHWEDLPVALAPDQPYDSGGCFS
                     GSAIERDGKLYLFYTGCQEIDGISHQHQCLAVSEDGIHFSKYSGNPVVAEKQLGENGL
                     IQEFRDPKVIVHNDGYYMVVATKSTQSTGRIVMFQSSDLYTWHFYSVLLEGTSEQGIM
                     WECPDLFHLDGRDVLIMSPIQIKKNGLEYHNISSTMACIGEVNWETGQMIVDNFHEMD
                     FGMDFYAPQTTRDPNGGRVMIAWMQMWDRTLPTHELGHLWAGSMSLPRELRVKKNRLV
                     QKPISTVYSHLVYQQGSENIAVTDTSVVFRNALSDSSYLHLVADLEKAEVFTIELLRS
                     KTDCLKLAYDTATQVFELDRQKLGRELTGAEEDPLNVRKVEVPLIDNQLVLEIFRDTS
                     SIEVFINGLEVMTATFYELEKARDILFHSIGESRIQAFETASVK"
     gene            9867..10859
                     /locus_tag="IV334_001900"
     CDS             9867..10859
                     /locus_tag="IV334_001900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005230454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAP8943962.1"
                     /translation="MKATMKDVAKLAGVGVGTVSRVLNNGSVKASTREKVEAAMTELN
                     YQPDFYARGLKMKHTNTVALILPTVWHPFFGEFAYHVETALERQNYKLYLCNSSGNSE
                     KEQEYIQMVSQNKVDGIIGITYSDIDEYISSNLPFVSIDRYFTEEVIYVTADNAAGGR
                     LAANELYRRGCQNVAYIGGYQETPNETKSRRKYFEIECRQLGISYKALDMLEPLDHLS
                     ERVTNFLLENPDIDGIFTINDPMALAVIDMLEKIGKNVPEDVQVIGFDGQKMTEGDPY
                     LVSTIAQPTKQMAEVAVAKLIAVINGETVEKRTVLPIEFGEGDTTKPLVRNDFA"
     gene            10911..11780
                     /locus_tag="IV334_001901"
     CDS             10911..11780
                     /locus_tag="IV334_001901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010748897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HAP8943963.1"
                     /translation="MLYGGIEAGGTKFVCGIGNEQLQVIERKSFPTTTPEETMEQVFD
                     FFDKYKGNIASIGVGAFGPIDIQIESRTYGYITNTPKLAWQNYHFVGALENALSVPIY
                     WTTDVNAACYGEYMAGYGKGKNSVLYYTVGTGVGGGAVVDGKIVAGFSHPEMGHMLLQ
                     RHNEDQFEGICPFHHDCLEGLASGPAIEKRFGRKAQTISFDHPYWTIEADYLAQCVYN
                     TTLLYAPEVIILGGGVMKQPQLLELIRQSFKEKMGSYVDTPTLEEYLLTPRLADNAGL
                     IGCLALAIKKLNH"
     gene            complement(12200..13765)
                     /gene="guaA"
                     /locus_tag="IV334_001902"
     CDS             complement(12200..13765)
                     /gene="guaA"
                     /locus_tag="IV334_001902"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HAP8943964.1"
                     /translation="MSSPTRFDEEPKIIVLDYGSQYNQLITRRIREFGVFSELMSHRI
                     TAEEVKAMNPKGIILSGGPNSVYDEKAFGIDEKIFDLGIPVLGICYGMQLITYKLGGK
                     VKPAKNREYGKAELEITSAEAALFQNTPEKQTVWMSHGDLVTQAPTDFDVVATSKDCP
                     IASIQNAERKMYGIQFHAEVRHSEYGNDLLRHFTLDVCQCTGDWTMDNFIDMEIAKIR
                     EQVGDKKVLLGLSGGVDSSVVGVLLQKAIGDQLTCIFVDHVLLRKGEAEQVMESLGGK
                     FGLNIIKVDAKERFLSKLAGVSDPEEKRKIIGNEFVYVFDDEATKLAGEEGVSFLAQG
                     TLYTDVIESGTETAQTIKSHHNVGGLPEDMQFKLIEPLNTLFKDEVRELGTQLGMPDS
                     IVWRQPFPGPGLGIRVLGELTEEKLEIVRESDAILREEIANAGLDRDIWQYFTVLPGI
                     RSVGVMGDGRTYDYTVGIRAVTSIDGMTADFARIPWEVLQKISVRIVNEVAHVNRIVY
                     DITSKPPATVEWE"
     gene            complement(13820..>14446)
                     /locus_tag="IV334_001903"
     CDS             complement(13820..>14446)
                     /locus_tag="IV334_001903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3-like element IS1476 family transposase"
                     /protein_id="HAP8943965.1"
                     /translation="LVMDMNASYCQLLKTVFPNAEIVTDRFHIVQHINRSFNQLRVQI
                     MNRFRTSHSENQKKYRRLKRYWKLLLKDSTTLEPLKRHYHRLFKRPISQTEIVDELLS
                     YNEELRTAYHFCQLLRYYFVKRGTEGFQETLKTISSTLPQSFKKKFTIFHRYQSGISN
                     AFKVSHSNGVTEGLNNKIKLIKRIAFGYRNFYNFRARIYLQQGLIFEN"
BASE COUNT         4412 a         2452 c         2991 g         4591 t
ORIGIN      
        1 ttgaaaaacg ttgatttatc ggcattcccc atccatatta tatttacgtc ttattcccac
       61 tcaatatgag tagcctcgta aagaagctat tatatcagtg ctttttcagc ctattcctca
      121 ttttggtagg gaaatggtag ggttagattt ttactgcttg atttccatta aagtttgcaa
      181 ctttagattt tgttgcacag gtaaatttaa tgtttcctgt catttgttct gcaattcctt
      241 catcattacg gctccataga tgagcgtagt gctttaaagt gatttcaggg ctagaatggc
      301 ctaaccgttg cgacacgacc agaatgtctg cgttaaactc attgattagg tagctaacat
      361 gagaatgacg taatcctttt ccttgtatta ggggtacctt agctagtttg gcataacgct
      421 gaataattcg ttgaacagtt gaacgataga gtgggaggtc agtgtagcta agaataaatt
      481 gttctacacc atgatttttt tgaataactt tccacttttt tagcacttca attgtatcat
      541 cgtccagaga aatgatccgc aagccgcttt cagtttttgt gtaaggtttc cgaataaaat
      601 ctttttgatt tgtcatttcc aaggtgtgat gaaccctcaa ctttttacgt ttgaagtcca
      661 cgtcattcca attaagtgct aatccttcgc ttacacgaac accagtcatg taatatagcc
      721 agatcattaa aaaacacata tgttcgtaga aatcatcttt ataaattttt gccaaaactt
      781 cttcaaattc ttcttttgtc cagtaaggaa cgattgattt tccttttgga attgcttttg
      841 ttcttttgga aatattctca ttcagaaatt gcagggtgac cgcatagtct aatgatttac
      901 gaaacatacc gtacataagt gaactgtatg cttgtgagta tccgctatcg ttcaataacc
      961 aaattcgata attttcacaa tctcgcacat tgatttcacg aagcttctta tcaccgaaaa
     1021 attcagtaat ctgaatcaat ccacactcac gagaactcca agtacttttt tgtacgctag
     1081 ctttgtagtg tggaacgtaa gactgctcca taaattcttt gtaagttaaa tcataattcg
     1141 caaatccatt tacaaccatg tattcatttt ttatacgcat gacttctttg tgtgcttcac
     1201 gagctgatga gaatcgtttt cctagagaat cttttgttcc tttcttctgg atcctctttc
     1261 cagtcacttg atctgttcct aactcaacta gataagaaaa gcgaccattt ttatcttgat
     1321 acacacaagg gtattttgtt ttcatagtta ttcctcctgt gaaagaggaa cacctataat
     1381 ttcttcaatt acttgtcggg gtactacccc aactctttta ttattataat atggatatcc
     1441 ctgatttacc attcttgttt ttgcttgtcg gattattgtg gtagcttgat gtgttttaaa
     1501 tcctagagcc tctaaatctt ttcgacaaat tgtatttttc attgtataaa ctcactttca
     1561 aagaaaacac tatcattggg taatagtgtt atttctttat ttttgtataa tgaacggtac
     1621 tttcttgaaa atccatcact tgttcaggac ttcccaactc atttaaagct aaagcattgt
     1681 aaaaaaatgg acgtctaact atatattcgc ttgtatatag tatatcttga ttcgtggaat
     1741 catagggttt ctttaccatc tttttggtga cagttggtag tttgagattc cgggacttga
     1801 aaaaagcttt tttcttatgc tctttcaatt ccagctcttt atcgaaatat ttgctgatat
     1861 agcgcccgcg attttcgata ctgtctacat cgatcttgtt gatcttaacg aaaccctgac
     1921 cccaaatcgt agttaaccgg ttaaatgaga taaaggggaa atcaaacaag ataatatggt
     1981 agtggattgc accgcgttgc tgcttttccc acgtggcgag atactttaaa tttgcttttt
     2041 tagttttata gaggtcataa ttcagtcgtt tgataaattt tttaaattca ttatttgcat
     2101 actcaatgtc ttgaatgttt tctttgaaag tgagagttaa aaatttcgtt tgattgtcaa
     2161 aattcgtgtc tacaaggcgg gcaatctcaa atcgcatact ttgataatgt ttctgtttgc
     2221 gttttaaact gtcatattgt ccttgagcgg ataattcatc atagtttttt cttttttcaa
     2281 gagtaatatc attttcataa atccaagcta atttttgttt tcgttccaga tttacttcgt
     2341 tttcgatcgt tttacgtttg ggagtaatga ttgcttcata atcataaatt tcaatatacg
     2401 tgggagtttc aatgattttc tgattataag cttgcaaaaa tcatcactcc ttgttgcgta
     2461 ttcgattgtc aaagaaccag tcattttctc ctgatgttga tctataaatc aagttaaggg
     2521 ggaaggcctg cgtagcagcc cttctagcct atggggtgtt gggcggtctt aacggacaga
     2581 aacggctctg tgggcgctgc cccgccgttt cgtcctaacc acccaacact ccatagtata
     2641 gttagttttg tgtagtggta ccttgttttc tgggaatcaa agttgccgta tcagcggttg
     2701 ccgataaccc ataactgaca aattgtccat tcgagcgtcc ccaaggtaca atcaccaaat
     2761 tggtaaatgt aattatggct aaaccttgtt gggtaagttc ttccggcgtg atcacaggat
     2821 gttcagattt gactttgaca ctgatttttt cgtagcgaag ttttggtagg atacattcgt
     2881 agctccaacc tagaatttcg ccctcctttt gccagcgagt gacattgacg acttggtatt
     2941 ctttttcgag tgtttctttt ggtaaactga gatcggtaat ttttattggc atcacaattc
     3001 tcctttttta tacgaaagtt tatacgattt gattaaaaaa tatagatggc catctacgac
     3061 ttgtattata tacgagaact tatacgtagt caagagattt tctaaaaaaa tatatacgat
     3121 tttttatacg agtgttataa tgaaaaaaat gataaggagg aatttgaata tgtttggttt
     3181 agtacaacct aataaagtga aggtgggcca acgaatcaaa gaaataaaag acggaatgtc
     3241 tctctctttt acagaattag ggaatcgatt aggtttaaaa aagccgacga tcagttccta
     3301 cgttcaaggc tatgcgttgg ctcctaagag tgtgatcaac caattatcta gtatcagcgg
     3361 aaaacctgtc ggctggtttt attttgggca tgtggaagaa tatatccgag agtatttgtt
     3421 tctcattggt cagaaaaaaa tagtggacga ttatcctgaa atcattgaga aaataaagca
     3481 agaattttat acgggcgagt tcaaaaatcc tgcttgggag aatgagctag gctatccttt
     3541 ggaggaattt atccaagaat gttttgtaga atattcgagt gaaatcatga aggactatgt
     3601 cgtacagatt acgaaagagg tacttgaaga atccaaaacg ctaaacaaac tatcatcaaa
     3661 agcaaaagaa gaagctgtca cgattctttc atctgggatc attgactata tcgagatgag
     3721 tggtgagatc aagtatggag agaaagatcg cattgcgaaa atggtgaaag aatcaataaa
     3781 aacctttgat gtgaaacaag acctgacatt taatgattcc tatttgattg gaaagttgat
     3841 caatattctt ggtacagatg aagatacgga gctgtttctt tcttctttat caatgatatt
     3901 tactgataag catttttcca ctcgatttgg tggggaagaa ttagtcgaaa tatttcaatc
     3961 gatgcgacca gcgttaatca aactctatcg ggagaaaaca ccagatgaat tttatgattg
     4021 gtttgagaag taaaataaga aaagtggctt agattacaag ttggtgaaaa aatttttttc
     4081 agcataacat cagtaatcta taaaggggtt gttgattata ctgtagaagt taagtactca
     4141 agagattttt tgtacgtaaa caaagcaaaa aagggtgcag tttcactaga aaatgtactc
     4201 ttttttgtga tttttcttag ttatagagtt tttttgaaac tttaaaaatg agaaaacata
     4261 ttgattttaa agagtgtaga catagcttct tttgctaaat agattaaact tactggtgag
     4321 tggtcttgaa catactttta aatcacattt tactgagttt tattggattt tataattcat
     4381 tggaaacctc gtacttaatc tgtttttatc tatcgactag aatctgtata tattccagct
     4441 atcaatgagg ccgcagaaaa gggtgcttaa aaataatctg ttgtttaaaa tattattatc
     4501 agaatactta gatacaaaat tctggcacaa agtaaaaaaa atattgactt aaaaaaatgg
     4561 atatggtaat ctataaacga aaatagtggt aacgtttcca aaatgcatct tcgttaaatt
     4621 tttatttttt taaacgaaaa tggaaacgtt tctaaaatgt attgggagga gattgaattg
     4681 gaaaagacat tgggaccagg tggtgagata agggcggtac aacgggataa aaaaaatatt
     4741 tcgaagtggg aaaaattaaa gaaacagttg cggcgtgata agtggatgta cttgttgctc
     4801 ttaccaggta tcatcatgac gtttatcttt aagtatgtgc caatgtacgg tgctgtgatt
     4861 gcatttaaag actataatcc tatgctagga attttggaaa gtccttgggt aggactagat
     4921 cactttaggg aatttatcag ttcaccaaat tttacgcttt tgctgagcaa cacattaaaa
     4981 attagtattt atggtttatt gttaggtttt tttccaccaa ttattttagc actgagcttg
     5041 aatcaaatta tcaataaaaa aataaaacag aaaatccagt tgattctata tgcgccgaat
     5101 tttatctcag tagttattat tgtggggatg ctgtttttgt tcctatccgc aaatggacct
     5161 attaatgggt tagtagaaaa ggttacgggg caaccaatta tgttcatgtc taatccagct
     5221 tatttcagaa cgatttatat tttatcggga atttggcaag gaatcggctg gtcatccatt
     5281 ctttacaccg caacgctggc gggagtcagt accgatttat atgaggcagc aaatatcgat
     5341 ggggcaaata ttttacaaaa gatttggcat atcgatttgc ctgcattgag gcctgtgatg
     5401 gtgatcagct tcatcttggc cgccggcggt attatgaatg taggatatga aaaagcctac
     5461 ttgatgcaaa cttcaatgaa catccctgcc tcagaaatca tttccactta tgtatacaaa
     5521 gtcggcttgc aatcaggtga ttatgcctac tctgctgctg ttggtctgtt caatatgatt
     5581 attaatgtcg ttttattact gattgtcaac aatataacca agcggctaaa cgatggtcaa
     5641 ggcttgtaat gaaaggagaa aattatgaat agtgctttat caaaaatcga ccgtcaaatc
     5701 ttatggttga atcgagtgat tgtaactttt ttagtcgtca tcaccattgt tccgctgatt
     5761 tatgtactgg tagcttcttt tatggaccca gctactttga tcaataaagg tataagcttc
     5821 aatccgaaag attggacaat acagggatat caacgggtat ttgcagatga ttccatttta
     5881 cgcggttttg taaattcgtt gttttactct tttagcttca gcttattgac tgtatttatt
     5941 acgatgatca ctgcgtatcc tttgtctcga cctaacttgg cggggaaaaa gattattatg
     6001 actttttttg tcattacaat gttcgtgggt ggtggcttga tacctaccta tttattggta
     6061 aagaacttgg gcatgttaga tagtgtatgg gcgattatcg taccaggtgc tataaatgtg
     6121 tggaatatta tcttagcccg tacctatttt agtgctttgc caaaagaact ttctgaagct
     6181 gctactatcg atggcgccaa tgacttgcaa atactgttca aagtcatgct tccattagcg
     6241 aaaccaatta tgttcgtatt gtttttatat gcctttgttg ggcaatggaa ctcatacttt
     6301 gatgccatga tttacatcaa agatcccgac ttacagccgt tgcaactcgt attaagaaat
     6361 attttgatcc aaaaccaacc ttcacaagac atggtgggag ccaatactgc catggcagaa
     6421 atgaaacaaa ttgctgagtt gatcaaatac gcaacgatcg tcatttctag tctgccatta
     6481 atcataatgt atcctttctt ccaaaaatat tttgataaag gtgttttagt tggttctgtt
     6541 aaagggtaag tattgttcac tatttgtatg aataaaaatt ataaaggaga ttttaagatg
     6601 aaagcaaaat ggatgctgcc aatcgttttt acttccgtac taattttggg ggcttgtgga
     6661 ggctcaaaag aaaagagtaa atcaagccct gattatgaac tagaaaatgt ctcttttcca
     6721 ttgaaagaaa aagtatcttt aaaaatgatg tctcaaagtg cccctttggc tcccaatgat
     6781 cctaatgaca aattgatttt ccaacgatta gaagaagaga ccaatctcca tattgattgg
     6841 acaaactaca gtagtgattt tgccgaaaaa agaaatttgg atatcgctag tggtgattta
     6901 ccagatgcca tcttcaacgc cgctgcaggt gactatgatt tattaaactg ggcggaaagt
     6961 ggcgttattg taccgattga agatttgatc gaagattata tgccaaatct gaagaaaatt
     7021 tttgatgaaa accccgaata ccgacaaatg tctactgcac ctgatggtca catttactct
     7081 atgccttgga ttgaagaatt aggcgaagga aaagagtcga ttcatacagt aaatgggatg
     7141 gcttggatca acaaggagtg gttggataat ttgggtcttg aaatgccgca aaccactgat
     7201 cagttgatga ctgtgttgga ggctttcaag acgcaagatc caaatggtaa tggtgaagca
     7261 gatgaaattc cttttagctt tattaacaat ggaggaaacg aagacttaaa atttttaatg
     7321 ggggcattcg gtctaggtga taatgatgac cacttagtcg ttgataacga tggtcaggta
     7381 caatttactg ctaacaaaga agggtataaa gaagccatca aatatttcaa tgaaatgaat
     7441 aataaaggtc ttattgataa agaggctttc gaacaagatt ggaatacgta tgttgccaaa
     7501 gggaaagaac ataagtatgg cgtgtacttt acttgggata aagcaaatat cacgggtgca
     7561 gatgatagtt atgacgtctt gccagtctta gcgggaccag atggaacgaa acatgttact
     7621 cgtacgaatg gcatgggttt ctcaagagat cgtttcgtaa ttactagtgc gaataaaaac
     7681 ttggaactta cagctaggtg gatagataaa atgtatgaac cgttacagtc tgtccaaaat
     7741 aactggggaa cctacggtga tgaagcacag gcgaatattt ttgaattaaa ggatggtatg
     7801 ttaaaacatt tgcctttaga ggggacagca ccgggggaac taagacaaaa aaccgaagct
     7861 ggaggaccat tagcagttct tgattcttat tacggcacag tcactactat gccagatgat
     7921 gcgaagtggc gtttagattt gatgcatgac acgtatttgc catatattac agaagacaat
     7981 atctatccac gtgtcttttt agaaaaagaa gaaacagatc gattagcaaa aatcgaaacg
     8041 gatatgtggg attacatcta tcgcaaaaga gcagagtgga tcaccggcgg taaaatcgat
     8101 gaagaatggg aagaatacct tgctgaattg gaacgtgtag gtttggaaga atggattcaa
     8161 ataaaacaaa ctggctatga taattttttg aagggagcac aataagtgtt agtagaagaa
     8221 acggcgattt tggataaagc aaatcgattt attgaaatga tgcgaggaaa agttaaccct
     8281 cagtatcgac aaaactatca tttttccgca cccatcgggt ggataaatga ccccaatggg
     8341 tttatatatt acaaagggca ttaccatctc ttctatcaat actatcctta cgatagtcga
     8401 tgggggccca tgcattgggg gcatgcgcgc agtagtgatt tggtccattg ggaagatcta
     8461 ccagtagcgt tagccccaga tcaaccttat gacagcggcg ggtgtttctc aggaagtgca
     8521 attgaaagag acggaaaact atatttattt tatacaggct gtcaagaaat tgatggtatt
     8581 agccatcagc atcaatgttt ggcggtctct gaagatggta ttcattttag caaatacagt
     8641 ggtaatcctg tggtagcaga aaaacagcta ggtgaaaatg ggctaataca agaatttcgt
     8701 gatcctaaag taattgttca caacgatggt tactatatgg tagtagctac taaatctaca
     8761 cagtctactg gtagaatagt gatgtttcaa tcgtcagatt tgtatacttg gcacttttac
     8821 tctgtgctgc tagaagggac ttcagagcaa ggaataatgt gggaatgtcc tgatttattc
     8881 catttagacg gacgagatgt cttaatcatg tctccgatac aaatcaagaa gaacggactg
     8941 gaatatcata atatcagttc taccatggct tgtattggtg aggtaaactg ggaaacaggt
     9001 cagatgatag ttgataattt ccatgaaatg gattttggaa tggattttta tgcaccacaa
     9061 acaacaagag atcctaatgg tggacgggtg atgattgctt ggatgcagat gtgggatcgt
     9121 acattgccta cacatgagtt aggccatctt tgggcaggct ctatgagttt gccccgagag
     9181 ttaagagtga aaaaaaatcg attggtacaa aaacccatct caactgtata tagtcattta
     9241 gtttatcaac aaggcagtga aaatattgca gtgactgaca catcagtcgt atttcgaaac
     9301 gcgctgtctg attcttcata tttacatcta gtcgcagatt tggagaaagc tgaagttttt
     9361 acaattgaat tactgcggtc taagacggat tgtctcaaac tagcctatga cactgctaca
     9421 caggtttttg aattggatcg tcagaaacta ggaagagagc ttacaggggc ggaagaagac
     9481 cctctgaatg tacgaaaagt tgaagtacct ttgattgata atcagctcgt tcttgagatt
     9541 tttagagata cctcatcaat tgaagtcttt attaatgggt tagaagttat gacggctact
     9601 ttctatgagc tggaaaaagc tcgagatatc ttgtttcatt cgatcggaga aagtcgtatt
     9661 caagcttttg aaacagcgag tgtgaaataa atgtttgatt gcttaggcga tgaatcaatt
     9721 atttaatagg ttttgaagga taatgttttt agaaaagaac agatagctag atttcccatg
     9781 gatttgctat tagtattttg aaaaaattag taatttatga caaaattcat tatactaaac
     9841 ttgtgcagaa atgtgaggga tcaactatga aagcaacaat gaaagatgtc gcaaaattag
     9901 caggtgttgg agtaggaact gtttcccggg tattgaataa cggttctgtt aaagcttcta
     9961 ctagagaaaa agttgaagca gcaatgacag agttaaacta tcaaccagat ttttatgccc
    10021 gggggttaaa gatgaagcat acgaacacgg ttgctttgat tctacccacg gtatggcatc
    10081 cattttttgg cgaatttgcc taccatgtag aaacagcatt agaaagacaa aactacaagc
    10141 tttatttgtg taactccagt ggtaatagtg aaaaagaaca agaatatatc cagatggtca
    10201 gccaaaataa agtagacggc atcattggaa ttacttattc agatatcgat gaatacattt
    10261 cgagtaatct gccttttgtt agtattgacc gttatttcac tgaagaggtg atttatgtga
    10321 ccgctgacaa tgcggccggt ggacgtttgg ctgcaaatga actttatcgc cgaggatgtc
    10381 agaatgtagc atatatcggt gggtaccaag aaacaccgaa tgaaacgaag agtcgtcgaa
    10441 aatattttga aatcgagtgc cgtcaactgg ggatttccta taaagcgtta gatatgttag
    10501 aacctttaga ccatctctca gaacgtgtaa caaatttttt gttagagaat cccgatatcg
    10561 atgggatttt tacgatcaac gatccaatgg cattagctgt cattgatatg cttgaaaaaa
    10621 tagggaaaaa cgtaccagag gatgtccaag ttatcggatt tgatggtcaa aagatgacag
    10681 aaggagatcc ttatctagtc tcaacgattg cccaacctac aaagcaaatg gcggaagtgg
    10741 cagtggcaaa attaattgct gtcattaatg gagagacagt agaaaaacgt accgtattgc
    10801 cgattgaatt tggcgaaggt gacacaacga aacctttagt gagaaacgat tttgcttagt
    10861 gcaaatacgt ttcttttttt gaaaatgagt gaatgaaagg tgtagataaa atgttatatg
    10921 gtggaataga agccggtgga acaaagtttg tctgtgggat tggtaatgaa caattacaag
    10981 tgatagaacg gaaaagtttt cctaccacaa caccagaaga aacgatggag caagtgtttg
    11041 atttctttga taagtacaaa ggaaatatcg catcaattgg tgtaggagca tttgggccta
    11101 ttgatattca aatcgaatca agaacttacg gatatatcac caacacaccg aagttagctt
    11161 ggcaaaatta tcattttgta ggagctttgg aaaacgcctt gtcggttccc atctattgga
    11221 ctaccgatgt aaatgctgct tgttatggag aatatatggc agggtatgga aaaggtaaaa
    11281 atagtgttct gtattacacc gttggtacag gtgttggtgg tggtgctgta gtggatggga
    11341 aaatagtcgc aggttttagt catcctgaaa tgggacatat gctcttacaa cgtcataatg
    11401 aagatcaatt tgaaggaatc tgtccttttc atcatgattg tttggaaggg ttagcatcag
    11461 gacctgcgat tgaaaaacgc tttggaagaa aagcccagac tatctcattt gatcatcctt
    11521 attggacgat tgaggcagat tatcttgctc agtgtgtgta taacacaacg ttactatatg
    11581 caccggaagt aataatttta ggcgggggtg taatgaaaca gccgcaacta ctcgagttga
    11641 ttcgccaatc gtttaaagaa aaaatgggtt cctacgtgga tacgcctact ctcgaagaat
    11701 atctactaac tccaaggctt gctgacaatg cgggcttgat tggctgccta gctttagcaa
    11761 taaagaaact caatcattag aatagtattt attagaacta ttcataacat accggaagtt
    11821 taaaagggta ttctgaaact taaatagtct caattcattc ttctttcttg agaatctgag
    11881 tagagacttt tattctgttt caaattaata taattacgaa atgatattga gtacaagaaa
    11941 gttcttttta ccagatcttt taaatcgaaa aatctgatat atatatttat agtactaaaa
    12001 atctgattag ataaaaagct aattcaatag gaaaataaag gtagtgaata aaagcaaagc
    12061 ccttgttagt agttaatcta acaagggctt tggtagggaa aatggtagga aaccatctta
    12121 ttttttacga aaatcaggaa ctttagtaat tgaataatgt tgatttatag gtattcctca
    12181 tccatattat gttggctctt tattcccact ccactgttgc aggtggtttg ctggtaatat
    12241 catacacgat acggttaacg tgtgcgactt catttacgat acggacactg attttttgca
    12301 agacttccca agggatacgg gcaaagtcag cggtcatgcc gtcaatagaa gtgacagcac
    12361 ggatacctac agtgtagtca tacgtacggc catctcccat aaccccaaca gagcgaatgc
    12421 ctggtaggac ggtgaagtat tgccagatgt cgcgatctaa accggcattg gcgatttctt
    12481 cgcgcaagat agcgtctgat tcacggacga tttctaattt ttcttctgtc aattcaccaa
    12541 ggacacgaat acctaagcct ggtccaggga atggttggcg ccaaacgatg ctgtcaggca
    12601 tgcctaattg ggtacctaat tcacgtactt cgtctttgaa caatgtgttc aatggctcga
    12661 tcagcttgaa ttgcatgtct tcaggtaatc cacctacatt gtgatgtgat ttgatggttt
    12721 gggcagtttc agttcctgat tcgatcacat cggtataaag ggttccttgt gcaaggaaag
    12781 aaacgccttc ttcaccggca agtttggttg cttcatcatc gaagacatat acaaattcat
    12841 ttccaatgat tttacgtttt tcttctggat cagacacgcc agcaagtttt gataagaaac
    12901 gttctttggc atcgactttt ataatgttca agccaaattt tccgccaaga ctttccataa
    12961 cttgttctgc ttcaccttta cgcaacaata cgtgatcaac gaagatacat gttaattgat
    13021 cgccgatcgc tttttgtaaa agaacaccaa caacagagga atctactccg cctgaaaggc
    13081 ctaataaaac ttttttgtca ccgacttgtt cacgaatttt tgcgatctcc atgtcgatga
    13141 agttatccat tgtccagtca ccagtacatt ggcagacatc tagtgtaaaa tggcgcaaca
    13201 agtcatttcc atattcggaa tggcgaactt ctgcatggaa ttggattccg tacattttgc
    13261 gttctgcatt ttggatcgat gcgatggggc aatctttgct ggtagcaacg acatcgaaat
    13321 cagtcggtgc ttgggtaact aggtcaccat gactcatcca aacggtttgt ttttcaggag
    13381 tgttttggaa aagggccgct tctgcagaag tgatttccaa ttctgctttt ccgtattcac
    13441 ggtttttggc tggtttaact tttccgccta atttataagt aatcaactgc ataccgtaac
    13501 agattcctaa aactgggatc cccaaatcaa aaatcttttc atcaatacca aaggccttct
    13561 catcataaac actgtttggt ccgccagaaa ggatgattcc tttagggttc attgccttta
    13621 cttcttctgc ggtaatgcgg tggctcatca actctgagaa aacaccgaac tcacgaatac
    13681 ggcgagtgat cagttggtta tactgactac cgtaatcaag tacgataatt tttggctctt
    13741 cgtcaaatcg tgttggtgaa gacaaatttg aataaaactt agtccgatat gcggcgcgtt
    13801 ctgtgcgccg catatttttt tagttttcaa agatcagacc ttgttgtaaa tagatccgtg
    13861 cccgaaagtt ataaaagttg cgataaccaa acgcaattcg tttaatcagc ttgatcttgt
    13921 tattcagccc ctcagtgacg ccgtttgagt gggacacctt aaaggcattt gaaatacccg
    13981 attggtagcg atggaagatc gtgaatttct ttttaaacga ctgaggtaac gtagatgaaa
    14041 ttgttttaag ggtttcttga aagccttccg tacctcgttt aacgaaataa tagcgtaata
    14101 attgacaaaa atgatacgca gttcgcaatt cttcgttgta gcttaacagc tcatcaacga
    14161 tttctgtttg actaattggt cgtttaaaca gtcgatgata atgccgtttg agtggctcca
    14221 atgtcgtgga atctttgagt aagagtttcc aatatcgctt gagccgacgg tacttcttct
    14281 gattttcaga gtgtgaggtt cgaaaacgat tcatgatctg gacacgtaat tgattgaagc
    14341 tgcgattgat gtgttggaca atatgaaagc ggtcagttac gatctccgca tttggaaaga
    14401 ctgttttaag caactgacaa tagctggcat tcatgtccat cactag
//
LOCUS       DACDKM010000070        14107 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20834_2016
            SAMN12640370-rid9891663.denovo.070, whole genome shotgun sequence.
ACCESSION   DACDKM010000070 DACDKM010000000
VERSION     DACDKM010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640370
            Sequence Read Archive: SRR10040891
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14107)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14107)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742835.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742835.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:04:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,012
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 29 of 75
            Pseudo Genes (incomplete)         :: 33 of 75
            Pseudo Genes (internal stop)      :: 19 of 75
            Pseudo Genes (multiple problems)  :: 5 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14107
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640370-rid9891663.denovo.070"
                     /isolate="E20834_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            66..767
                     /locus_tag="IV334_001904"
     CDS             66..767
                     /locus_tag="IV334_001904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenine-N(1))-methyltransferase"
                     /protein_id="HAP8943966.1"
                     /translation="MNHLDLSQRLAAVGDFVPESARLADIGSDHAYLPVALILRGKIE
                     YAVAGEVVQGPYESARKQVKKDGLEDRITVRLANGLDAIQPQDGITAVTIAGMGGSLI
                     RDILEAGKLNQRLSGQERLILQPNIGERTLRSWLQENQYKIQSEKILEENKKIYEIIV
                     AEKEQKPVTYNEMELMFGPFLLEEKSEIFKSKWTRELQQRKAIREQLKQAANQEARQK
                     QIEQEIQMIEEVLKP"
     gene            764..1885
                     /locus_tag="IV334_001905"
     CDS             764..1885
                     /locus_tag="IV334_001905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HAP8943967.1"
                     /translation="MTISGRTFIQKFESYCPQWLAEEGDPVGLHIGTLDKPIQRVMMT
                     LDVRPEVVEEAIKKKIDLLIAKHPPIFRPVKRLVTDQPQEKMYADLLKHDIAVYAAHT
                     NMDIIWDGLNDWFCELLGIEVESYLVKTHEIHYKKLAVYVPVDHAQKMREVLAAAGAG
                     TQGDYTGTSFTSIGHGRFTPEAGAQPAIGKVGKTEQVQEAKVEVILPETIEKQVIQAM
                     RSAHPYEEPAYDLFAIDEPVEMFGLGRVGELPQEISIEAFVEQVKEAFQLDGLRIVQP
                     KNAKSSVKRIAICGGSGEKFYPQAIAQRADVYITGDIYYHTAHDMQSAGLIAIDPGHY
                     IESLCKQRFIEKFESWKQEENWDINFFVSETNTNPFQFN"
     gene            1900..3132
                     /gene="pepT"
                     /locus_tag="IV334_001906"
     CDS             1900..3132
                     /gene="pepT"
                     /locus_tag="IV334_001906"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HAP8943968.1"
                     /translation="MYENLLPRFLRYVKTETRSDATSKTTPSTQTQVAFAQELKKELE
                     ELGLADVFYNEDNGFVMGTLPSNVDHEVRSIGFISHMDTADFNAVGVNPQIIENYDGE
                     STIVLDAEERYTLNTKDFPNLKNYEGHTLITTDGSTLLGADDKSGIAEIMTAMEILIN
                     NPSIPHGTIKVAFGPDEEIGVGADKFDVEQFDVDFAYTVDGGPLGELQYETFSAAQAN
                     ITIQGKNVHPGTAKDTMINALQLAIDFHNQLPADEVPEKTEGYEGFFHLMALDGNPEE
                     ASMSYIIRDHDREKFEARKAQITHIQEELNQRFDQERIKVDMYDQYYNMKEIIEKDMS
                     IVELAKQAMIELGISPVIEPVRGGTDGSKISYMGIPTPNLFAGGENMHGRFEFVSLQV
                     MEKATDVIVKIAELNTKS"
     gene            3264..4172
                     /locus_tag="IV334_001907"
     CDS             3264..4172
                     /locus_tag="IV334_001907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YegS/Rv2252/BmrU family lipid kinase"
                     /protein_id="HAP8943969.1"
                     /translation="MEKLLVFYNKSSGKDEGEKLARWFQDYVKEKQPELYVELAETGP
                     SIDDAELVKKAEAIEADTLVIIGGDGTIHHIVQAFQKTLSQYHVGLLPGGTVNNLARV
                     LEIPLKKEEAADVILEGNVRKIDYGQVNDEVIISTLTIGILADTAVWISQEEKQRYGS
                     WIFIKKFFKLLLKKKKYHLDLETDEEHWKGKSQLLTVTMSNSVGGFTNFDDSATLDDG
                     KFHVTIVPSLDIFRFIFYLPRIMRGKFYTIPGITYFTAKRMKIQAQEKSVGTRTDGDT
                     TDDLPVELSVVSKGLTVYSPKKEEKA"
     gene            4207..4479
                     /locus_tag="IV334_001908"
     CDS             4207..4479
                     /locus_tag="IV334_001908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1294 domain-containing protein"
                     /protein_id="HAP8943970.1"
                     /translation="MDVLLHNLLWFYLFIVNFYLFCLMGYDKHQARTSGWRVPENNLI
                     FVSLLGGGLGGLISRKVFHHKTRKKKFTIGFAAGVLVDLILIYFFH"
     gene            4490..5536
                     /locus_tag="IV334_001909"
     CDS             4490..5536
                     /locus_tag="IV334_001909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flippase-like domain-containing protein"
                     /protein_id="HAP8943971.1"
                     /translation="MSSKGTKTKIILNLLLLIVIFAIIYYLINQSFADIFAELLSTSV
                     VVLAAAVILGTVYQIAEGRSIKEIAEPFAPNFTTKDGFLTSSYIAFYRIVSFGTGTLI
                     SEIYFYNKKGMKLSQGAGVTALHMIMYKLAVITYALAGLVIQFSLFYSNGPSMIWFII
                     AGILLTIVIVAFLLAMSVSLNVQIFFVSLSNKLFKSERLRKWVDTCNQQIYSLRETVQ
                     TIIQDRTALLRIYLWNMLKLVFWYVLPYLILIENHPKIDFLLTISFISFAVVLSGVIP
                     TPAGIGSFEFVYLLLFRPVVGTVDAVSSLLLYRFSSFILPFLYGFVYVIAERRRVIKE
                     EIIEVKKENKENQG"
     gene            5787..8396
                     /gene="clpB"
                     /locus_tag="IV334_001910"
     CDS             5787..8396
                     /gene="clpB"
                     /locus_tag="IV334_001910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HAP8943972.1"
                     /translation="MNIEKMTTTLQEAIAEAQQIAVTRKHQDIDIAHVWKIFLQPNHF
                     GRNFYTDAGLDVESFEHEIDRLLDEYPVVSGGNVQYGQNLSQNFFRLLNEADQIRESF
                     GDEFLSTEVVILALMKLKNYPLTVYLNKNGLSEKELRKNIEEMRGGERVTSQNQEEQY
                     KALEKYGVDLVQQVKSGKMDPIIGRDEEIRDVIRILSRKTKNNPVLIGEPGVGKTAIV
                     EGLAQRIVRKDVPENLKDKTIFSLDMGALIAGAKFRGEFEERLKAVLKEVKKSDGRIL
                     LFIDEIHNIVGAGKTEGSMDAGNLLKPMLARGELHLIGATTLDEYRQYMEKDKALERR
                     FQKVLVKEPTVEDTISILRGLKERFEIHHGVNIHDNALVAAATLSDRYITDRFLPDKA
                     IDLIDEASATIRVEMNSMPTELDQVTRRLMQLEIEEAALKKESDDASKKRLKNLQEEL
                     AELREEANAMKMQWETEKEEVNSVSAKRAEIDKAKHELEDAENNYDLERAAVLRHGTI
                     PQLEKELKELEAKAKDSEIKMVQESVTENEIAQVVGRLTGIPVTKLVEGEREKLIKLN
                     ETLHKRVIGQDEAVDAVSDAVIRSRAGLQDPNRPLGSFLFLGPTGVGKTELAKALAEN
                     LFDSEDHMVRIDMSEYMEKHAVSRLVGAPPGYVGYEEGGQLTEAVRRNPYTIVLLDEI
                     EKAHPDVFNILLQVLDDGRLTDSKGRVVDFKNTVLIMTSNIGSQLLLEGVTADGTIPE
                     AVAEQVNTLLRGNFKPEFLNRIDDTILFTPLSLDNVKGIVDKMVAQLAQRLEHQEILL
                     TISDEAKTWIAENAYEPAYGARPLKRFITKEVETPLAKEIVAGHVMPKSKVTITLLDG
                     QLHFKTEELEEIV"
     gene            complement(8547..9218)
                     /locus_tag="IV334_001911"
     CDS             complement(8547..9218)
                     /locus_tag="IV334_001911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8943973.1"
                     /translation="MIKEIKKVQIALLAFGVFVVCYNFYEFITQKYSTSQGITFIVES
                     LLGIALIFMPQVILKVFKLKIPAAIVLFYWFFLFISVFLGTGMHLISIISFWDKILHA
                     VSPMVLTALGYGLIGYLMKDAEISKTSPWLFLLFGFAFAGLCGVFWEFWEFLCDQFLG
                     MNLQRFAASDGTLFVGRAALMDTMGDLLTNTIGAALMGLFAWSQSKKDERYFESYKLE
                     KVKNT"
     gene            complement(9211..9705)
                     /locus_tag="IV334_001912"
     CDS             complement(9211..9705)
                     /locus_tag="IV334_001912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deoxyribosyltransferase"
                     /protein_id="HAP8943974.1"
                     /translation="MKIYFAAPMFAKSDLLYNQQLVKEIRELSSELSIYLPQENEAIN
                     DKTAYADSRMIALADTEKVLASDLMIALLDGLTIDAGVASEIGVAYAKGIPVIGLYTD
                     TRQQGGTHPKKIAALQETAENQFHYLNLYTIGLIKLNGKVVSSEIELLSEVKRFLDGG
                     TFSD"
     gene            complement(9725..10945)
                     /locus_tag="IV334_001913"
     CDS             complement(9725..10945)
                     /locus_tag="IV334_001913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP8943975.1"
                     /translation="MDQKDFRENLELKPVTQEHIDQFNELLSYVFQVTEADIEESGFE
                     NKRAFIRSKKPILEVSKVFGWFHENKLISQIAIYPCEVNIHGARYKMGGVTGVGTYPE
                     YANHGLMQDLIIVALDNMRKNKQWISYLYPYSIPYYRRKGWEIMSDKLSFKIRDTQLP
                     KQIPVSGMVERLPVDHPDVFSVYDKFAQQNHGALFRSDFHWEEYWRFENEDERTAAVY
                     YDSNHEPRGVLFYWVAEEIFHVKEMFYLDQEARNGLWNFISAHFSMIYWVHGDIYKNE
                     PLAFLIEDSQIKEQIEPYFMARIVDVKEFLQRFPFVGTADAFHFIIEDPVAPWNNGVF
                     ALTWDEQGQVRVLNEPIGKSVRLDIQTLTCLMMNYRRASYLARIERLETDEETLKSLE
                     RIIPNMEAYFSDYF"
     gene            11092..12927
                     /gene="lepA"
                     /locus_tag="IV334_001914"
     CDS             11092..12927
                     /gene="lepA"
                     /locus_tag="IV334_001914"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor 4"
                     /protein_id="HAP8943976.1"
                     /translation="MDIKKMKERQEQIRNFSIIAHIDHGKSTLADRILEMTHTVTSRE
                     MQDQLLDSMDLERERGITIKLNAVELNYTAKNGETYIFHLIDTPGHVDFTYEVSRSLA
                     ACEGAVLVVDAAQGIEAQTLANVYLALDNDLEILPVINKIDLPAADPERVRQEIEDVI
                     GIDASEAVLASAKSGIGIEDILEQIVEYVPAPSGDLDAPLKALIFDSIYDSYRGVVLN
                     VRITDGMVKPGDKIKLMSNGKTFDVTEVGVFSPKAVARDFLMVGDVGYITASIKTVQD
                     TRVGDTVTLADNPAAEALPGYRKMNPMVYCGLYPIDTSRYNDLREALEKLQLNDAALQ
                     FEPETSQALGFGFRCGFLGLLHMDVVQERLEREFNLELITTAPSVIYHVNKTDGTTAT
                     VDNPADFPEPVTIQDVEEPFVKAQIMVPNDFVGAVMELSQRKRGEFITMDYLDDYRVN
                     VVYNIPLSEIVFDFFDKLKSSTKGYASLDYEMSGYQKSKLVKMDILLNGEKVDALSFI
                     VHRDFAYERGKAIVEKLKKLIPRQQFEVPIQAAIGQKIVARSDIKALRKNVLAKCYGG
                     DVSRKRKLLEKQKEGKKRMKQIGSVEVPQEAFMAVLKMDEDEPKK"
     gene            complement(13021..14103)
                     /locus_tag="IV334_001915"
     CDS             complement(13021..14103)
                     /locus_tag="IV334_001915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017414133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAP8943977.1"
                     /translation="MNDDGSRKKVEKVGGDTRPEAEAALRKVLSDIDETGQYFLGTDT
                     RVKQYLDFWMEEYVKLNLKYNTYENYRFTIKNHINGYLGKKKLTDLSPALLQNFINAE
                     FKKGYSKKTMTITHSVLKNALNMAVYPWGLIKQNPMLYVKIPKYEARPTTKKDLKIIS
                     LEDFDHMLEITPEGHPFYIPLNIGFYTGMRVGEVCGLTWDDVDFSNGTITVEKQMVKN
                     DGAWVYGTPKTSSSNRTIFIGQTLLAILKKHKKQQLENRMKYGKLYIDSNAVCTKEDG
                     ELVTPSVVKWNTRRISNALSLSFNFHSLRHTHATLLLENGAKMKEISERLGHSRISIT
                     MDTYSHVTDKMRNETVDIMENLRKNS"
BASE COUNT         4555 a         2592 c         2919 g         4041 t
ORIGIN      
        1 ttttttattc ctagtctcct tattttattt ggaaaatgct ataattatct agaaggagtg
       61 aagctatgaa ccatttagat ttatctcagc gcttagccgc agtgggtgac tttgtccctg
      121 aatccgctcg tttagcagac attggatcag atcacgctta cttacccgtt gcattgatat
      181 taagaggaaa aatcgaatat gcagttgctg gtgaagtcgt tcaaggtcct tatgaatcag
      241 cacgtaagca agtcaaaaaa gatggattag aagaccgtat caccgtacgc cttgcaaatg
      301 gtctagatgc tatccagcca caagatggta ttacagctgt cacgattgcg ggtatgggag
      361 gttcactgat ccgagatatc ctagaagctg gcaagttgaa tcaacgctta agcggacaag
      421 aacgtttgat ccttcagcca aacatcggag agcgtacatt acgatcttgg ctgcaagaaa
      481 atcagtataa aatccaatca gaaaaaatac ttgaagaaaa taaaaaaata tatgaaatca
      541 ttgtagccga aaaagaacaa aaaccagtta cttacaatga aatggagctg atgttcgggc
      601 catttctgct tgaagagaaa agtgagatct tcaaatcaaa atggacaaga gagcttcaac
      661 aaagaaaagc tatccgtgaa cagctcaaac aagcagctaa tcaagaggca cgtcaaaaac
      721 aaatcgagca ggaaatccaa atgattgagg aggttttaaa gccatgacaa tcagcggacg
      781 cactttcatc caaaagtttg aaagctattg tcctcagtgg ctagctgaag aaggagaccc
      841 agtcggactg catatcggaa cattggataa gccaatccaa cgagtaatga tgacattaga
      901 cgttcgtcca gaagtggtcg aagaagcaat caagaaaaaa atcgatttgc tgattgctaa
      961 acatcctcca atctttcgtc ctgtaaaacg gctggttact gatcagccac aagaaaaaat
     1021 gtatgcagat ttgttgaaac atgatattgc cgtctatgca gcacacacga acatggatat
     1081 tatttgggat ggactgaatg actggttttg tgaacttctc ggtattgaag tggagagtta
     1141 tttagtaaag actcatgaga ttcattataa aaaactggcc gtttatgtac ctgtggatca
     1201 tgcacagaaa atgcgtgaag tattagctgc agctggtgct ggtacacaag gtgattatac
     1261 cggtacaagt tttacctcga ttggacacgg tcgatttact ccggaagctg gtgcacaacc
     1321 agcgattggg aaagtaggga agacagaaca agttcaagag gcaaaagttg aggttatttt
     1381 gccggaaact atcgaaaaac aagtgattca agccatgcgg tcagctcatc cttatgagga
     1441 acctgcttat gatcttttcg ctatagatga accagtcgaa atgtttgggt taggacgagt
     1501 aggtgaattg ccgcaagaaa tttctataga ggcatttgta gaacaagtaa aagaagcgtt
     1561 ccaactagat ggactgagaa tagtccagcc aaaaaatgca aaatcctctg tgaagcgcat
     1621 tgcgatttgc ggtggaagcg gtgaaaaatt ttatccgcaa gcaatcgccc aaagagcaga
     1681 tgtctatatc acgggagata tctattatca tacggctcat gatatgcaaa gcgctgggtt
     1741 gattgcaatt gatcccggtc attatattga atcgttatgc aagcaacgat ttatcgagaa
     1801 atttgaatct tggaaacaag aagaaaattg ggatattaac tttttcgttt ctgaaacaaa
     1861 tacaaaccca tttcaattta attaaaagga gagaatacta tgtacgaaaa tcttttgcca
     1921 cgttttttac gatatgtcaa aacagaaaca cgctccgatg caacaagcaa aacgactcca
     1981 tcgactcaga cacaagttgc ttttgcgcaa gaattgaaaa aagaattaga agagttaggc
     2041 ttagctgatg ttttctataa tgaagataac ggattcgtta tgggaacatt accaagcaat
     2101 gttgatcatg aagtgcgttc cattggattt atttcccaca tggatacagc agattttaat
     2161 gcagtaggag tcaatccaca aatcattgaa aactatgatg gcgagtctac gatcgtacta
     2221 gatgcggaag aacgctatac attgaataca aaagattttc caaatttgaa aaattatgaa
     2281 ggccacacat tgatcacaac agatggcagt acattactgg gagcagatga caaatcaggg
     2341 attgcggaaa tcatgactgc aatggagatc ttgatcaata atccttctat ccctcacggt
     2401 accatcaaag tggcttttgg tcctgatgaa gaaatcggtg tcggagcgga taaattcgat
     2461 gttgagcaat tcgatgtaga ttttgcttat acagtcgatg gtggtccttt aggtgagttg
     2521 cagtatgaaa cgtttagtgc tgctcaagca aatattacga tccaagggaa aaatgtacat
     2581 ccagggacgg caaaagatac aatgatcaat gctctacagt tagcaatcga ttttcataat
     2641 cagcttccag ccgatgaagt tccagaaaaa acggaaggat acgaaggttt cttccatttg
     2701 atggcattgg atgggaatcc tgaagaagct tctatgagtt atatcattcg cgatcatgat
     2761 cgtgaaaaat tcgaagcacg caaagcacaa atcacacata tccaagaaga gttgaatcag
     2821 cgatttgatc aagaacgaat caaagtagat atgtatgatc aatattacaa catgaaagaa
     2881 atcattgaaa aagatatgag catcgtggaa ttagcgaaac aagcaatgat cgagcttggt
     2941 atctctccag tgatcgaacc agttcgtgga ggaacagatg gttcaaaaat ctcttacatg
     3001 ggtattccga cgccaaatct atttgccgga ggagaaaata tgcacggacg attcgaattt
     3061 gtttctttac aagtaatgga aaaagcaaca gacgtgattg taaaaattgc agaattgaac
     3121 acaaagagtt aaaatcgaaa aagaacagct tatgacaagg cgttcgtccc aatgatggaa
     3181 tgtcatctgt ctaaggctaa agggcaggac aatggaattg gttgtcccgc cctctttttt
     3241 gaagagtaaa taggaggtaa agaatggaaa agctgctagt tttttataac aaaagttcag
     3301 ggaaagatga gggtgagaaa ttagcacgct ggtttcaaga ttatgtaaaa gaaaagcagc
     3361 cagaattata tgtagaacta gctgaaactg gcccatcgat tgatgatgcg gaattagtca
     3421 agaaagcaga agccatagaa gctgatactt tagtaattat aggtggagac ggcacgattc
     3481 atcatatcgt ccaagcgttc caaaaaacat tatctcaata ccatgtcgga ttacttccag
     3541 gtggaactgt caacaacctt gcacgtgttc ttgagattcc cctcaaaaaa gaagaagctg
     3601 ctgacgtgat cctggaaggc aatgtacgga aaatagacta tggtcaagtc aatgatgaag
     3661 tcattatctc tacactgaca atcggaatct tagcagatac agcagtttgg atcagtcaag
     3721 aagaaaaaca gcgttacggc tcgtggatct ttatcaagaa gttttttaag ttattgctga
     3781 agaagaaaaa atatcatttg gatttagaaa cagacgagga gcattggaaa ggaaaatccc
     3841 aactgttgac agtcacgatg tctaactccg tgggcggttt tacaaatttt gacgactcag
     3901 cgaccctaga tgatggaaaa tttcatgtaa cgatcgtacc ttccttagat attttccgat
     3961 ttatttttta tttgcctaga atcatgagag gcaaattcta tacgatcccc ggcattactt
     4021 atttcacagc caaacgcatg aaaatccaag cgcaggaaaa atctgtgggc actcgaacgg
     4081 atggagacac gacagatgat cttccagttg aattatctgt tgtatcaaaa ggattaacag
     4141 tttattcacc aaagaaagaa gaaaaagctt agaaatagta gttaacagaa agttggaaga
     4201 agagtaatgg atgtattgtt acacaatctt ttgtggtttt accttttcat tgtgaatttt
     4261 tacttatttt gtctgatggg gtatgataaa catcaggcta gaactagcgg ctggcgtgtt
     4321 cctgagaata atttgatatt tgtcagcctg ttaggcggtg gtttgggagg attgatctct
     4381 cgaaaagttt tccatcataa aactcggaaa aagaagttta ctataggatt cgctgcaggt
     4441 gtacttgttg atttgattct catttatttt ttccattaag gagcaggaga tgtcgtctaa
     4501 aggaacgaaa actaaaatca ttctgaatct gcttttgctg attgtgattt ttgccattat
     4561 ttattattta atcaatcaat catttgcaga tatctttgcc gaattgctgt ctaccagtgt
     4621 tgttgttttg gcagcggctg ttatattagg gacagtttac caaatcgctg aagggcgctc
     4681 aatcaaagaa attgctgaac ctttcgcgcc gaattttaca acgaaagatg ggttcttgac
     4741 ctctagctat attgcgtttt accgaatcgt ttcgtttggt acaggaacgc tgatctcgga
     4801 gatttatttt tataacaaaa aaggaatgaa gctatcccag ggagcagggg tgacagcact
     4861 ccatatgatt atgtacaaac tggcagtgat cacttatgca ttagctggct tggtcatcca
     4921 attctctctt ttttattcta atggtccatc aatgatctgg tttatcatcg ctggtatcct
     4981 tttgacgatc gtgattgttg cctttttatt ggcgatgtcc gtcagcttga atgttcaaat
     5041 attttttgtc agcttatcga ataagttatt taaaagtgag cgtttgcgga aatgggtgga
     5101 tacatgcaat cagcagatct actctttaag ggaaacggtc caaaccatta tccaagacag
     5161 gactgcgcta ttgcggattt atctatggaa tatgttaaaa cttgtatttt ggtatgtact
     5221 tccttatttg attctgattg aaaaccatcc taaaatagat tttctactga ccatttcgtt
     5281 tatcagcttt gcagtagtgc tttcaggggt tattcctacg cctgctggta ttggttcctt
     5341 tgaatttgtc tatttgttat tattcagacc agttgttgga acagttgacg cagtttcttc
     5401 gcttttactg tatcgtttca gttcctttat cttgccattt ctctatggat ttgtgtatgt
     5461 gattgctgaa cggcgaagag tgatcaaaga agaaataata gaagtaaaaa aagagaataa
     5521 agaaaatcaa gggtaggtct tttataagat ctaccctttt tatcaaatca aagaaaaacg
     5581 ttatggataa gataatttta cgtttgtttg gctctttttt tatatacata caatcaattt
     5641 tgttttttta agatttatat aagtcgagaa aaaaagtcaa aaaactatgt gtatattttg
     5701 accaaaattg accaaagcga tataatagtt tttgtaaaga tcaaagaaat aagtgatctt
     5761 acctttaatt aggagggtat acctgtatga atattgagaa aatgactacg acgcttcaag
     5821 aagcgattgc tgaagctcaa cagattgcgg tcactcgaaa acatcaagat atcgacattg
     5881 cccatgtatg gaaaatcttt ttacaaccta atcattttgg tcgaaatttc tatacggatg
     5941 ccggattgga tgtcgaatct tttgaacatg aaattgatcg tctactggat gagtatccag
     6001 ttgtttctgg tggaaacgtc caatatggac aaaacctcag ccagaatttc tttcgattat
     6061 tgaatgaagc agaccaaata cgtgaaagct tcggcgacga gtttctttca acggaagtcg
     6121 ttattttagc gttgatgaaa ttaaaaaatt atccgttaac tgtttatttg aataaaaacg
     6181 gattatctga aaaagaattg cgtaaaaaca tcgaagagat gagaggagga gaaagagtga
     6241 cctcacaaaa ccaagaagaa caatacaaag cattagaaaa atacggcgtt gatttagtac
     6301 agcaagtcaa aagcggaaaa atggatccaa tcatcggtcg tgatgaagaa atccgtgatg
     6361 tcatccgtat tctttcacga aaaacaaaaa ataatcctgt tctgatagga gaacccggtg
     6421 tagggaaaac ggcaatcgtt gaagggcttg ctcaacgtat cgtacgcaaa gatgtgcctg
     6481 aaaatttaaa agataaaacg attttttcat tagatatggg tgcattgatc gctggagcaa
     6541 aattccgtgg tgaatttgaa gaaagactaa aagcagtctt gaaagaagtc aaaaaaagtg
     6601 atggacgtat cctcttgttc atcgatgaaa tccacaatat cgtaggggca ggaaaaacag
     6661 aaggaagtat ggatgccgga aatctgttga aaccaatgct tgctcgcggt gaattacatt
     6721 tgatcggtgc cacaacttta gatgagtacc gtcaatatat ggaaaaagac aaagcgttgg
     6781 aacgtcgctt tcaaaaggta ttagtcaaag aaccaacagt ggaagatacg atcagtattc
     6841 tgcgtggatt aaaagaacgt tttgagatcc accatggtgt taacatccat gacaacgcat
     6901 tagtagcagc agctacttta tctgaccgtt atattactga tcggttctta ccggacaaag
     6961 caatcgattt gatcgatgaa gccagtgcaa cgattcgggt agaaatgaac tctatgccaa
     7021 cggaattaga tcaagttact agacgtttga tgcagttgga aatcgaagaa gctgcactga
     7081 aaaaagaatc agatgatgca agtaaaaaac gcttgaaaaa tttgcaagaa gaattagctg
     7141 aacttcgtga agaagcaaat gcgatgaaga tgcagtggga aacagaaaaa gaagaagtca
     7201 attctgtttc agcaaaacgt gctgaaatcg ataaagcaaa acacgaatta gaagatgcag
     7261 aaaataacta tgatttagaa cgcgctgctg tattgcgtca tggtacgata cctcaattag
     7321 aaaaagaatt gaaggaatta gaagctaaag caaaagacag cgagattaag atggttcaag
     7381 agtccgttac cgaaaatgag attgctcaag tcgttggcag attaacgggt atccccgtaa
     7441 cgaaacttgt agaaggtgaa cgagaaaaac taattaaatt gaatgaaaca cttcataaac
     7501 gagtgattgg tcaagatgaa gcagttgatg cggtcagtga cgcggtaatt cgttcacggg
     7561 ctggattgca agacccaaat cgtcctctag gttctttcct tttcctagga ccaactggtg
     7621 tcggaaaaac cgaattagcg aaagcgttag ctgagaactt gtttgactca gaagatcata
     7681 tggtacggat cgatatgagt gaatatatgg aaaaacatgc tgtgtctcgt ctagtcggag
     7741 ctcctccagg ctatgtaggt tacgaagaag gtggtcaatt aacggaagct gtcagacgaa
     7801 atccttatac aatcgtcttg ttggatgaaa tcgaaaaagc gcatcccgat gtcttcaata
     7861 tcttgttaca agtcctagat gacggacgct tgactgattc aaaagggcga gtagtagatt
     7921 tcaaaaatac ggttctgatc atgaccagca atatcggttc acagttactg ctggaaggcg
     7981 tgactgctga tggaacgatt ccagaagcag tggctgaaca agtcaacacg ctattacgtg
     8041 gaaacttcaa accagaattc ttgaaccgaa tcgacgacac gattttattt acaccattga
     8101 gcctagacaa cgtgaaagga atcgttgaca aaatggtggc gcaattggca caacgtctgg
     8161 aacatcaaga aatcctgctg acaatcagtg atgaagccaa aacgtggatt gctgaaaatg
     8221 cttatgaacc agcatatggt gcaagaccat tgaaacgatt tatcactaag gaagtagaaa
     8281 caccactagc taaagaaatc gttgcaggac atgttatgcc taaatcaaaa gtaacgatta
     8341 ctttacttga tggacaactt catttcaaaa cagaagagct agaagaaatc gtctaatctt
     8401 ccattgagac tataaaagaa aaaaggggtc tgacacaact agtgtcagac ctcctttttt
     8461 caaataaaca gtgttccaga agtagtttct tcgaaaaata agccgaaatt tcttgaagct
     8521 gcccgcttct gtcacaacct cttttattag gtatttttca ctttttctaa tttataactt
     8581 tcaaagtaac gctcatcttt tttactttga gaccatgcaa acaatcccat taaggcagca
     8641 cctatcgtat ttgtcagcaa gtcgcccatc gtatccatca atgctgctcg tccgacaaac
     8701 agtgtgccat cagaagcagc aaaacgttga agattcatgc ctaagaactg gtcgcataag
     8761 aattcccaga actcccaaaa cacgccgcaa agtccagcaa acgcaaagcc gaataagagg
     8821 aacaaccaag gactggtttt tgatatctca gcatctttca tcaagtaacc aatcaatcca
     8881 tagccaagag ctgtcaggac cattggactc accgcatgta agattttgtc ccaaaaagag
     8941 atgatactga tcaaatgcat ccctgtgcct aaaaagacag agatgaacaa gaaaaaccag
     9001 taaaacaaaa cgattgctgc tggtatttta agtttgaaaa ctttcaaaat gacttgtggc
     9061 atgaaaatca atgcaatccc taataaagat tccacgataa atgtgattcc ttgagaagtc
     9121 gaatattttt gtgtgataaa ttcataaaaa ttatagcaga caacgaatac accaaaagca
     9181 agtaaagcta tttgcacctt tttgatttct ttaatcactg aaagtccctc catctaaaaa
     9241 tctttttact tctgaaagca actcaatttc tgaagaaacc acttttccgt tcaatttgat
     9301 caaacctatt gtatataagt tcaggtagtg aaattgattt tcagctgttt cttgtagtgc
     9361 agcgattttt ttcgggtggg ttccgccttg ctgtcgagta tcagtatata agccgatcac
     9421 tgggatgcct ttcgcataag ccacaccgat ttccgatgct accccggcat cgatcgtcag
     9481 cccatctagt aaagcaatca ttaaatcact agccaacact ttttctgtgt cagcaagagc
     9541 aatcattcga ctgtctgcat aagcagtttt gtcattgatt gcttcatttt cttgaggaag
     9601 atagatcgaa agctcagaag agagctctct gatttcttta actaactgtt ggttatataa
     9661 caaatcactt ttagcaaaca ttggtgcggc aaaataaatt ttcatctaaa catcatcctt
     9721 tttatcaaaa ataatcgcta aagtaagctt ccatgtttgg aatgatacgc tctagtgact
     9781 tcagtgtttc ttcatctgtt tctaaccgtt cgattcttgc caaataagaa gcacggcgat
     9841 aattcatcat caagcaagtc aatgtttgga tatccagtcg aacggatttt cctataggtt
     9901 catttaagac acggacttgt ccctgttcat cccatgttaa agcgaagaca ccattattcc
     9961 aaggagcgac aggatcttca atgatgaaat gaaaagcatc agctgttcca acaaacggaa
    10021 accgttgaag gaattctttt acatcaacga tccgagccat aaaatatggt tcgatctgct
    10081 ctttgatctg actatcttca atcaaaaatg ctaaaggctc atttttataa atatctccat
    10141 gtacccagta gatcatggaa aaatgtgcgg aaataaagtt ccataagcca tttcgagctt
    10201 cttgatcaag atagaacatt tcttttacgt gaaatatttc ttcagctacc caatagaaca
    10261 gaacacctct tggttcatgg ttgctatcgt agtagacagc ggctgtgcgc tcatcttcat
    10321 tttcgaaacg ccaatactct tcccaatgaa aatcactgcg gaataaagca ccatggtttt
    10381 gttgggcaaa tttgtcataa acggaaaata cgtctggatg atcaactggt aatcgctcaa
    10441 ccataccact tactgggatt tgtttaggta gctgtgtatc tctgatttta aaggaaagtt
    10501 tatcagacat gatttcccat cctttacgcc gatagtaggg gatactgtaa ggatacaaat
    10561 aagagatcca ttgcttattc ttgcgcatat tatccaatgc aacgataatt agatcttgca
    10621 tcaatccatg gttagcatat tctggataag ttccaactcc agtcacacca cccattttat
    10681 aacgtgcacc gtgaatattg acttcacaag gatagatagc gatttgagag atcagtttat
    10741 tttcgtggaa ccatccgaac actttcgata cttccaaaat cggttttttg gaacggataa
    10801 aagctcgttt attctcaaac ccgctttcct cgatgtctgc ttcagtcact tggaacacat
    10861 aggaaagcaa ttcattgaat tgatcgatat gttcttgagt gaccggtttt aattcaagat
    10921 tttctctaaa atctttttga tccatttttt catctccatt tattagcttg ataacagtat
    10981 agcaaaattc tacaaaaaaa acttctatag ctgactaatt aaaaactctt taaattactg
    11041 cttttcgttg gtataatgga aagtgctgat tatatgaaaa gaggagacgc tatggatata
    11101 aagaaaatga aagaacgcca agagcagatt cgtaattttt cgatcatcgc tcatatcgac
    11161 catgggaaat ctacattggc cgatcgtatt ttagaaatga cgcacactgt gacgtctcgc
    11221 gaaatgcaag atcagctgtt agactcgatg gatttggaac gtgaacgtgg aatcacgatt
    11281 aaattaaatg ctgtagaact caattacaca gcgaaaaatg gagaaacata tatctttcac
    11341 ttgattgaca ccccagggca cgtcgacttc acctatgaag tctctcgaag ccttgctgcc
    11401 tgtgagggtg ccgttttagt ggtcgatgca gcacaaggaa tcgaagcaca aacattggcc
    11461 aatgtttatt tagcgttaga caatgatttg gagatcctgc cagtcatcaa taaaatcgac
    11521 ctacccgcag cagatcccga acgtgtccgt caagaaatcg aagacgtaat cggaatcgat
    11581 gctagtgaag ctgtattagc cagtgccaaa tcagggattg ggattgaaga tattctcgag
    11641 cagatcgtgg aatatgttcc tgcaccaagt ggagacttag atgcaccgct aaaagctttg
    11701 atctttgact ctatttatga tagttaccgc ggtgttgttc taaatgtccg gatcacggac
    11761 ggtatggtca aacccggaga caaaatcaaa ctgatgagta atggcaaaac atttgatgtt
    11821 acagaggtcg gggtcttctc gccaaaagca gttgctagag actttttgat ggtcggtgat
    11881 gtcggttata tcacagcaag tatcaaaact gtccaagata cacgagtagg ggatacagta
    11941 acgttagcag acaatcctgc cgctgaagct ttaccaggtt accgaaaaat gaatccgatg
    12001 gtttattgtg gtctttatcc aattgatact tctcgttaca acgatttgcg ggaagcttta
    12061 gaaaaactgc aattgaatga tgctgcttta caattcgaac cagaaacctc tcaagcatta
    12121 ggttttgggt tccgctgtgg attcctcgga cttcttcata tggatgtcgt ccaagaacgc
    12181 ttggaacggg aattcaatct agaattgatc acgactgcac catccgttat ttaccatgtt
    12241 aataaaacgg atggtacgac tgctacagta gataacccgg cagattttcc tgaacctgta
    12301 acgattcagg atgtagaaga accattcgtc aaagcacaga tcatggtccc aaatgatttt
    12361 gtcggtgcag taatggaact ttctcaacga aaacggggag aattcattac gatggattat
    12421 ttagatgatt accgagtaaa tgtcgtctat aatattccgc tttctgaaat tgtctttgac
    12481 tttttcgaca aattgaaatc aagcacgaaa ggctatgcct cattggatta tgaaatgtct
    12541 ggttatcaaa aaagcaagtt agtgaagatg gacatcttgc tgaatggaga aaaagtcgat
    12601 gcattaagct ttatcgttca ccgagacttt gcctatgaac gtggaaaagc aatcgttgaa
    12661 aaactgaaaa aactaatccc acgccaacaa tttgaggtgc caatccaagc agctattgga
    12721 caaaaaatcg ttgcccgttc agacatcaaa gccttgcgta aaaacgtctt ggctaaatgt
    12781 tatggtggag atgtttctcg taaacgtaaa ctcttagaga aacaaaaaga agggaagaag
    12841 cggatgaaac aaatcggctc cgtcgaagtt ccgcaagaag ccttcatggc cgttctgaaa
    12901 atggacgagg acgaaccgaa gaaatagcaa gggtttgttt gaaaacagtg taaaaaataa
    12961 accatgttta catggatttg ccaccggttt gccaccggtg gtttttttcg gtggcaaaaa
    13021 tcaagaattc tttctaagat tttccatgat atcgaccgtt tcatttctca tcttatctgt
    13081 tacatgcgag taagtatcca tcgtaattga aattctgctg tggcccaatc gttcagagat
    13141 ttctttcatt tttgcgccat tttcgagaag aagtgtagca tgagtatgtc tgagagaatg
    13201 gaagttaaaa gagagggaga gtgcattcga tatcctccta gtattccatt tcactacact
    13261 tggcgtaact agctcaccgt cttccttcgt acatactgca tttgaatcaa tgtagagctt
    13321 tccatacttc attcgatttt ctaattgttg tttcttatgt tttttcagaa ttgctagcaa
    13381 ggtttgtcca ataaaaatcg ttcgattgga actgcttgtc tttggcgtac catataccca
    13441 tgcgccgtca ttctttacca tttgtttctc tacagtaatt gttccatttg aaaaatcgac
    13501 atcatcccac gtcagaccac aaacttcgcc aacgcgcatt cccgtataaa atccaatatt
    13561 caaaggaata tagaaaggat gtccttcagg agtaatttct agcatatgat caaagtcctc
    13621 aagagaaatg atttttagat cttttttagt cgttggtcgt gcttcatatt ttggtatttt
    13681 tacatacagc ataggatttt gcttgattaa tccccaagga taaaccgcca tattcagcgc
    13741 attcttaagg acagagtgag taatagtcat tgttttcttc gagtaaccct ttttaaattc
    13801 agcattgatg aaattttgta aaagagcagg ggagagatcc gtaagttttt tctttcctaa
    13861 ataaccgttt atatgatttt tgatggtaaa tcggtagttt tcataggtat tgtattttag
    13921 atttagttta acgtattcct ccatccaaaa atcaaggtat tgttttactc gagtatccgt
    13981 acctaaaaag tattgtcctg tttcgtcaat atctgataaa acttttcgta aagcagcttc
    14041 ggcctctggt cgggtgtctc cgccaacttt ctccactttt tttcttgagc catcatcatt
    14101 gatatct
//
LOCUS       DACDKM010000071        14045 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20834_2016
            SAMN12640370-rid9891663.denovo.071, whole genome shotgun sequence.
ACCESSION   DACDKM010000071 DACDKM010000000
VERSION     DACDKM010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640370
            Sequence Read Archive: SRR10040891
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14045)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14045)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742835.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742835.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:04:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,012
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 29 of 75
            Pseudo Genes (incomplete)         :: 33 of 75
            Pseudo Genes (internal stop)      :: 19 of 75
            Pseudo Genes (multiple problems)  :: 5 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14045
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640370-rid9891663.denovo.071"
                     /isolate="E20834_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(574..1509)
                     /gene="whiA"
                     /locus_tag="IV334_001916"
     CDS             complement(574..1509)
                     /gene="whiA"
                     /locus_tag="IV334_001916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein WhiA"
                     /protein_id="HAP8943978.1"
                     /translation="MSFAADVKKELTGLEVHREHAKAELAALLRMNGSLGIVNQQFIL
                     NVQTENAAIARRIYSLLKDHYQVRSELLVRKKMKLKKNNVYIVRLKQGTKDVLMDLDI
                     MDGMMFNGHVSDEIMGNSQKMRSYLRGAFMASGSVNNPETSRYHLEIFSIYEQHNQDI
                     CDMLNYYGLHARTLERRNGYISYLKGAEKIADFLTLIGATNSMLRFEDVRIVRDMRNS
                     VNRLVNCETANLNKTIDAASKQIENIELIEAKVGLHALPEKLQEIAELRLQHPEVSLK
                     ELGEMIPSGAISKSGINHRIRKINEFAEKLREEAV"
     gene            complement(1543..2541)
                     /locus_tag="IV334_001917"
     CDS             complement(1543..2541)
                     /locus_tag="IV334_001917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YvcK family protein"
                     /protein_id="HAP8943979.1"
                     /translation="MKMKTYRIRKPKIVVVGGGTGLPVILKSLRNQGADITAVVTVAD
                     DGGSSGAIRESIAMAPPGDLRNVLVALSDMPQFYEDIFQYRFKKEDQFLANHTIGNLI
                     IAAVSEMRGSTYEAIQLLAKMMHVDGHVYPSSETPLTLHAVFKDGTTAVGESKIAINR
                     KTIDRVYVCNTNDKSEAKAARKVVSAIMDADMVVLGPGSLFTSILPNLVIPEIGEAML
                     KTTAETVYICNIMTQKGETEHFTDADHIRVLHKHLNQPFIDTVLVNTEKVPEDYMDPE
                     IYDEYLVQVAHDFNGLRNEGCRVISTDFLELKDGGVFHDGDKVVEELFRLVFGSKY"
     gene            complement(2538..3422)
                     /gene="rapZ"
                     /locus_tag="IV334_001918"
     CDS             complement(2538..3422)
                     /gene="rapZ"
                     /locus_tag="IV334_001918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HAP8943980.1"
                     /translation="MTDNLQLVIITGMSGAGKTVAIQSFEDMGYFCIDNMPPRLIPKF
                     WELIKESGKVTKMALGVDLRSRSFFEEIQNMLIEIENTNFIDTSILFLDASDEELVAR
                     YKETRRAHPMAMDGLITEGIRKERAILDDLKAQASVIIDTTTLTPRQLREKINESFKS
                     KGDQGFRIEFVSFGFKYGLPIDSDIVMDVRFLPNPHYIPELRPLTGKDQPVYDYVMSF
                     DETQSFYHKFLDLLETVLPGYIKEGKSSLTVAIGCTGGQHRSVALTERLANALGKNYK
                     VNVTHRDKDKRKETVNRS"
     gene            complement(3557..4288)
                     /locus_tag="IV334_001919"
     CDS             complement(3557..4288)
                     /locus_tag="IV334_001919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate racemase"
                     /protein_id="HAP8943981.1"
                     /translation="MENFFSILGGMGTMATESFVRLINHRTKATKDQEYLNYVLFNHA
                     TVPDRTAYILDRSEENPMPFLLDDIEKQNLLRPNFIVLTCNTAHYFFEELQAATDIPI
                     LHMPREAANELVRQHTTGRVAILGTEGSMKAGIYEREVKNLGFETVIPDTALQEKINY
                     LIYHEIKESDYLNQELYYEILEEAVERLNCEKVILGCTELSLMHEFAEDNHYPVIDAQ
                     SILADRTIERALAERSEALDTASEK"
     gene            complement(4292..5557)
                     /locus_tag="IV334_001920"
     CDS             complement(4292..5557)
                     /locus_tag="IV334_001920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-aspartate ligase"
                     /protein_id="HAP8943982.1"
                     /translation="MMNSIENEEFIPILLGSDMNVYGMARSFNEAYGKICQAYASDQL
                     APTRYSKIVNVEVIPGFDKDPVFIETMLRLAKERYSDKSKKYLLIACGDGYAELISQH
                     KQELSEYFICPYIDYSLFERLINKVSFYEVCEEYDLPYPKTLIVREEMLVNGHLEQEL
                     PFEFPVALKPANSVEYLSVQFEGRKKAFILETREEFDLILGRIYEAGYKSEMIVQDFI
                     PGDDSNMRVLNAYVDEDHQVRMMCLGHPLLEDPTPASIGNYVVIMPDYNEKIYQTIKA
                     FLEKIEYTGFANFDMKYDPRDGEYKLFEINLRQGRSSFFVTLNGLNLARFVTEDRVFN
                     KPFVETTYGTNQSDKARLWMGVPKKIFLEYARENEDKKLAEQMIKENRYGTTVFYEKD
                     RSIKRWLLMKYMFHNYIPRFKKYFHVKEG"
     gene            complement(5820..8636)
                     /gene="uvrA"
                     /locus_tag="IV334_001921"
     CDS             complement(5820..8636)
                     /gene="uvrA"
                     /locus_tag="IV334_001921"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HAP8943983.1"
                     /translation="MANDKITIHGARAHNLKNIDVTIPRDKFVVVTGLSGSGKSSLAF
                     DTLYAEGQRRYVESLSAYARQFLGQMDKPDVDSIDGLSPAISIDQKTTSKNPRSTVGT
                     VTEINDYLRLLFARVGHPICPNDHIEITSQSVDQMADQVMALPERTKIQVLAPIVVKK
                     KGQHKKIFERIQKEGYVRVRVDGEIYDLSEVPELEKNKKHDIAIVIDRIIVKEGIRSR
                     LFDSLEAALRLADGYALVDVIGEEEMLFSEHYACPYCGFTVGELEPRLFSFNAPFGAC
                     PDCDGLGVKLEVDQDLVIPDRTKTLSQGAIVPWNPISSQYYPQMLAQACESFGIDLDT
                     PFEELPEEHQEIILNGTDDLFHFHYENDFGGVRDVETTFEGVLKNIERRYHDTNSDFT
                     REQMRLYMTELTCQTCKGYRLNPQALSVQINGTNIGQTNELSIQKAVDFFSNLTLSEQ
                     EKVIAKPILKEVNDRLSFLENVGLDYLTLSRASGTLSGGEAQRIRLATQIGSNLSGVL
                     YILDEPSIGLHQRDNDRLIGSLKKMRDLGNTLVVVEHDEDTMWAADYLIDVGPGAGEQ
                     GGQIVAAGTPEEVANDENSLTGKYLSGKKSIPVPKERRKGNGKAIKITGASENNLKNI
                     SVEFPLGEFVAVTGVSGSGKSTLVNSILKKSLAQKLNKNSAKPGKFKTISGYESIEKI
                     IDIDQSPIGRTPRSNPATYTSVFDDIRGLFAQTNEAKMRGYKKGRFSFNVKGGRCEAC
                     RGDGIIKIEMHFLPDVYVPCEVCHGKRYNSETLEVHYKGKSIADILEMTVEDAVEFFK
                     HIPKIHRKLQTIVDVGLGYVTMGQPATTLSGGEAQRMKLASELHKISNGKNFYILDEP
                     TTGLHSDDIARLLHVLQRLVDAGNTVLVIEHNLDVIKTADYIIDLGPEGGEGGGTILA
                     TGTPEEIINVKESYTGHYLKKIMV"
     gene            complement(8646..10640)
                     /gene="uvrB"
                     /locus_tag="IV334_001922"
     CDS             complement(8646..10640)
                     /gene="uvrB"
                     /locus_tag="IV334_001922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HAP8943984.1"
                     /translation="MIERDTSRHFELVSKYQPAGDQPEAINQLVDGVVGGKKAQILLG
                     ATGTGKTYTISNLIEKVNKPTLIIAHNKTLAGQLYGEFKEFFPNNAVEYFVSYYDYYQ
                     PEAYVPSSDTYIEKDSSVNDEIDKLRHSATSSLLERNDVIVIASVSCIFGLGSPFEYQ
                     KQVVSIRQGAELDRNQLIRDLVSIQFERNDIDFQRGRFRVRGDVVEIFPASRDERALR
                     VEFFGDEVERIREVNALTGEVLGETEHVAIFPATHFVTNDEHMEHAVANIKAELEQRL
                     TVLRNENKLLEAQRLEQRTNYDIEMMLEMGYTSGIENYSRHMDGRKEGEPPYTLLDFF
                     PEDFLIVADESHVTMPQIRGMYNGDRARKQMLVDYGFRLPSALDNRPLRLEEFEKHVN
                     QIIYVSATPGPYEHEQTDTVIQQIIRPTGLLDPVIEVRPIMGQIDDLVGEINERVEKD
                     QRVFVTTLTKKMAEDLTDYFKELGIKVKYLHSDIKTLERTEIIRDLRLGEFDVLVGIN
                     LLREGLDVPEVSLVAILDADKEGFLRSERSLVQTIGRAARNEEGKVIMYADKVTDSMR
                     LAMDETSRRRTIQQKYNEEHGIVPKTIIKEIRDLISITKESEDDTKEAVQVSYEEMTK
                     EEKDTLLMKLEKEMKDAAKALDFETAANVRDMILELKASK"
     gene            10899..12401
                     /locus_tag="IV334_001923"
     CDS             10899..12401
                     /locus_tag="IV334_001923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294446.1"
                     /note="The N-terminal region of this protein, as described
                     by TIGR01726, is a three transmembrane segment that
                     identifies a subfamily of ABC transporter permease
                     subunits, which specificities that include histidine,
                     arginine, glutamine, glutamate, L-cystine (sic), the
                     opines (in Agrobacterium) octopine and nopaline, etc;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HAP8943985.1"
                     /translation="MKRKHLFHSFVFLMTGLVTFAFGHVAQADNTTTAESSTSETMQK
                     IEPKKDVYVIASDSAFAPFEFQNAKGEYEGIDVKLMENIAKLQGFNIRMDYRGFSAAL
                     QALESGQADGMIAGMTVTDERKKSYDFSDSYFDSGIQLAVKKGDTSIKSYEDLKGKTV
                     GAKVGTESAEFLSDHESEYGYSIKYFDAADQLYDGLKVGAIDAMMDDYPVIGYAIKQG
                     QELETPIKKESGGKYAFAVKKGQNPELLNMFNEGLKELKRTGEYDKIIKEYVADGSET
                     SSESSTADESTFLGLIQNNYQALLKGLWRTILLTLISFALAIVIGVIFGLFSVAPIRA
                     LRTIASIYVDIIRGIPMMVLAFFIFFGLPGIVGFTIPDFLAGVITLTLNASAYIAEIV
                     RGGINAVPVGQMEASRSLGLSYNRTMQKIILPQAIRIMIPSFVNQFVISLKDTTIISA
                     IGVVELLQTGKIIVARTTQSTYVYLIIAIMYLILITALTKLAKVLEKKVK"
     gene            12403..13140
                     /locus_tag="IV334_001924"
     CDS             12403..13140
                     /locus_tag="IV334_001924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010760672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HAP8943986.1"
                     /translation="MAEKILVEHLVKKYGDNTVLNDINVSINEGDVVCIIGPSGSGKS
                     TFLRCLNRLEEPSDGEIIIDGAHLMDKSTDINLVRQHIGMVFQHFNLFPHLSILENII
                     LAPMDLKKESREEAEKKAIKLLQTVGLEDKKDMYPEMLSGGQKQRVAIARALAMNPDI
                     MLFDEPTSALDPEMVGDVLNVMKKLAEQGMTMVIVTHEMGFAKEVANRVMFIDDGNFL
                     EDGTPQQIFENPQNERTKDFLNKVLNI"
     gene            complement(13185..13358)
                     /locus_tag="IV334_001925"
     CDS             complement(13185..13358)
                     /locus_tag="IV334_001925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HAP8943987.1"
                     /translation="MEKKNQAQLSEGGIDYVLDIINEPMVLSYAMEQVERLLVCAGKQ
                     AMRLIRLGEKIRR"
     gene            13334..13999
                     /locus_tag="IV334_001926"
     CDS             13334..13999
                     /locus_tag="IV334_001926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar diacid utilization regulator"
                     /protein_id="HAP8943988.1"
                     /translation="MELDFSSPYYVVVGQLSDNQQLCQERINPLLELLERVFSYYGQA
                     LLTVHRQQIIILVNRISRASLLSLLDKIQTKFNKMYQLQLNFGIGSLCYTEQETPQSF
                     LHAKQVCEWIAFHQSVNEIRFFEDLDLGIVLPAIPSDQRTLYVKRILKSLTEEEVHLF
                     KKTLACFSKNNGSIKNCSEELFIHKNTLQYRLNKFHSLTGYTPRNYDDYHILKLAFLL
                     VQT"
BASE COUNT         4046 a         3031 c         2436 g         4532 t
ORIGIN      
        1 agcacatttt ttgccctccc tttataaaca tcgattctca aattctaaac caaatcttga
       61 tagttcaagt tcaggtataa cgtataccaa ccatacatcc caattactat ttatgaactt
      121 atgattatca ctgaataaat cactccatcc ccttgttaaa acttctacat taattacacc
      181 tggttgtttt cgactagcta atccaatgaa cttatcaacc atccttatcc accaaccacg
      241 agaatcttga ccagaagtaa gaaaaacagt tctcaaggtt gatcgagcag ttccttccag
      301 tttttaatta atctccagtt atagataaat acttgatcct atctatatca ggacttctat
      361 attaagcaaa agtttatgta taaaactaaa atcttcattc agttgtctcc ccgcttttta
      421 tacgattgta ttaaaaaaca atagaagcct ttcaacatta tgttactcca gtaaagttgg
      481 aattatagtt tgacggaaga aaagcaagta tagcttttaa aaaaaactga tacattcgca
      541 tctcctgctt catgtatcag ttttttcttt ggattaaact gcttcttcac gcagtttttc
      601 cgcaaattca tttattttgc gaattcggtg attaattccc gattttgata ttgcacctga
      661 gggaatcatt tcccctaatt ctttcaagct gacctctgga tgttgaagcc gaagttctgc
      721 gatttcctga agtttttcag gaagggcatg tagaccgacc tttgcttcga tcaattcgat
      781 gttttcgatt tgtttagaag ccgcatcaat cgtcttgttc agatttgctg tctcacaatt
      841 gactagacga ttgaccgaat ttctcatatc ccgtacgatt cgaacatctt caaatcggag
      901 cattgagttg gttgcaccaa tcaatgttaa gaaatcagca attttttctg ctccttttaa
      961 atacgaaata tagccattgc gccgttcaag cgttcgagca tgtagaccgt aataatttaa
     1021 catatcgcaa atgtcttggt tatgctgttc ataaatagaa aatatttcca aatgataacg
     1081 actcgtttcc ggattattga ctgaacctga ggccataaaa gcaccacgta agtaagaacg
     1141 cattttttgc gaatttccca taatctcgtc cgaaacgtgt ccattgaaca tcataccatc
     1201 catgatatca agatccatca acacatcttt tgtgccttgc ttcaaccgga cgatatagac
     1261 attgttcttt tttaatttca tttttttacg aacgagtagc tcacttcgta cctgataatg
     1321 atctttcaaa agagaataga tacgtctggc aattgcagca ttttctgttt ggacgttcaa
     1381 aataaactgc tggttcacaa tacctaaaga gccgttcatt cgcagcaaag cagctaattc
     1441 cgctttcgca tgttctcgat gaacttccaa accagttagt tctttcttca catctgcagc
     1501 aaatgacatc ctattctcct cctttctcca acaataaatt gcttaatatt ttgaaccgaa
     1561 aactaaacgg aacaattctt ctactacttt gtctccatcg tggaagacac caccatcttt
     1621 caattctaaa aagtcggtgg aaatcactcg acatccttca tttctcaatc cgttgaagtc
     1681 gtgtgcgact tgtactagat actcatcata gatctctggg tccatataat cttcaggcac
     1741 tttttccgta ttgaccaata ctgtatcgat aaaaggctga ttcaaatgct tgtgtaaaac
     1801 acgtatatga tcggcatctg tgaaatgttc cgtttcccct ttttgggtca tgatattaca
     1861 gatatagacg gtttcagcag ttgtcttgag cattgcttcg ccgatttctg gtatcactaa
     1921 atttggaagg atactggtga ataaacttcc gggtcccaaa acgaccatgt cggcgtccat
     1981 aattgctgaa acgacttttc gagccgcttt ggcttcactt ttatcatttg tgttgcagac
     2041 atacacacgg tcaatggttt tacggttgat cgcgatttta gattctccta ctgcagtcgt
     2101 tccgtcttta aaaacagcat gcaaggtcaa aggagtttct gaagaaggat aaacatgtcc
     2161 atctacatgc atcatttttg ctaataattg aatcgcctca taagtgcttc cccgcatctc
     2221 ggaaactgcg gcaatgatca gattaccgat cgtatgattg gctaaaaact gatcttcctt
     2281 tttgaaacgg tattggaaaa tatcttcata gaattgtggc atatccgata acgccactaa
     2341 gacatttctc aagtcaccag gaggagccat agcgatcgat tctctgattg caccgctgct
     2401 tccaccatca tctgcaacag tcactactgc tgtgatatct gctccttgat ttcgtaaact
     2461 tttcaggata actggcagac cagtgccacc gccgaccact acgatctttg gttttcttat
     2521 ccggtaggtt ttcattttca cgaccggttc accgtctctt tccttttatc cttgtcccga
     2581 tgtgtcacat tgactttata gtttttccct aaagcatttg ccaaacgttc agtcaatgcc
     2641 acagaacggt gttgaccgcc tgtacagccg attgcaactg tcaagctgct ttttccttct
     2701 ttaatgtatc caggcaatac agtttccaga agatccaaaa atttatggta gaatgattgc
     2761 gtttcatcaa aggacatcac ataatcatag actggctgat cttttccggt caatggtctt
     2821 agttccggga tataatgagg gttgggcaaa aaacgcacat ccatcacaat atccgaatcg
     2881 atcggcaacc cgtatttaaa accaaatgat acaaattcga tccgaaatcc ttggtctccc
     2941 tttgatttga atgactcatt gattttttca cgtaattgtc gtggtgtcaa tgttgtcgta
     3001 tcaatgatca cggaagcttg ggctttcaag tcgtctagta tcgcccgttc tttacgtatc
     3061 ccttcagtga tcaaaccgtc catcgccatt ggatgtgctc gtcttgtttc tttgtagcga
     3121 gccactagtt cttcgtctga agcatctaaa aataagatag aagtgtcgat gaagttcgta
     3181 ttttcgattt cgatcagcat gttttggatt tcttcaaaga aggaacgaga acgcaaatcc
     3241 actcctaaag ccatttttgt gactttacct gattctttga tcaattccca gaattttgga
     3301 atcaatcgag gcggcatatt atcgatacaa aaatagccca tatcttcaaa actttggata
     3361 gctactgttt tccctgcacc actcatacct gttatgatga ctaattgcaa attatctgtc
     3421 atatttacga acccctctca cattgtgcat aataaaatta tatcatcccg ggcgtatcat
     3481 taccagtttt ctttttcttt tacaaaaaga tttggaccaa atatctttta ggatatttgc
     3541 cccaaatcca atttttttac ttttccgatg ctgtatccaa tgcctcgctt ctttcagcta
     3601 atgcacgttc gatcgttcga tccgctaaaa tagattgagc atcgatcact gggtaatgat
     3661 tatcttcagc aaattcatgc atcaaagata attctgtgca acctaggatg actttttcac
     3721 agtttaggcg ctctaccgct tcctctaaga tttcgtaata gagctcttga ttcaaataat
     3781 ccgattcctt gatctcatga tagatcaggt aattgatttt ttcttgaagc gcagtatcag
     3841 gaatcactgt ttcgaaacca agatttttga cttcgcgttc atagattcca gctttcatac
     3901 ttccttctgt tcctagaatc gctactctcc ctgtcgtatg ctgacggaca agttcatttg
     3961 ctgcttctct tggcatatgc aagataggaa tatccgttgc cgcttgtaac tcttcaaaaa
     4021 agtagtgtgc ggtgttacaa gtcaacacaa tgaagtttgg tcgcaataaa ttttgtttct
     4081 caatatcatc cagcagaaac ggcattggat tttcttctga tcgatctaaa atataagctg
     4141 tacgatcagg aactgttgca tgattgaata agacataatt caaatattct tgatcttttg
     4201 tagctttcgt tcggtggttg atcaagcgaa caaaactttc tgtcgccatc gtgcccattc
     4261 cgcctaaaat actgaaaaaa ttctccatga actagccttc tttcacatga aaatactttt
     4321 tgaatcttgg aatatagtta tggaacatat atttcatcaa caaccagcgc ttgattgatc
     4381 ggtctttttc ataaaataca gtagtaccgt aacgattttc tttgatcatt tgctcagcta
     4441 gttttttgtc ttcattttcc cgagcatatt ctaaaaagat ttttttgggc acacccatcc
     4501 ataatcttgc tttatctgat tgattcgttc cataggtcgt ttcaacaaag ggtttgttga
     4561 acactcgatc ttctgtgaca aaacgagcca gattcaagcc gttcaatgtc acaaagaaac
     4621 tgctacgacc ttgtctcaaa ttgatttcaa ataatttata ttctccatct ctaggatcat
     4681 acttcatatc gaaattggca aagcctgtat attcgatttt ttccaaaaat gccttaatgg
     4741 tttgatagat tttttcgtta taatctggca tgatcacaac gtaattacca atcgatgctg
     4801 gtgtaggatc ttctaatagt ggatgaccaa ggcacatcat ccgaacttga tgatcttcat
     4861 ctacatacgc atttagcaca cgcatattac tatcatcacc cgggataaaa tcttgtacaa
     4921 tcatttcact tttataaccg gcttcgtata tccggcctaa aattaaatcg aactcttctc
     4981 tagtctctaa aataaaagct ttttttcttc cttcaaattg gacagaaaga tactccacac
     5041 tgtttgcagg tttcaaagca actgggaatt caaaaggcaa ttcctgttcc aaatgtccat
     5101 ttactaacat ttcttctctg acgatcaatg ttttaggata cggaagatca tattcttcac
     5161 aaacttcata aaagcttact ttattgatca gcctttcaaa taaagaataa tcaatatatg
     5221 gacagataaa atattctgac agctcttgtt tatgttggga gatcaattct gcatatccat
     5281 caccacaagc aatcaaaaga tattttttac tcttatcaga atatctttct tttgccaaac
     5341 gaagcatcgt ctcgataaat acagggtctt tatcaaatcc tggaataact tctacattga
     5401 caattttact gtagcgggta ggggctaact gatccgaagc atatgcttgg cagattttgc
     5461 cgtatgcttc attaaaagaa cgtgccatac cataaacgtt catgtcgctt cctaataata
     5521 tagggataaa ttcttcattt tcaatactgt tcatcattat ctctcactta ctcataatct
     5581 cttgtttttc ttttccaact acacaaggaa tatcatacca taaagaaaaa agcaatcaaa
     5641 ggaaagtcct ccgattgctc aattcttttt tgaacggatt gaacttatag gaagttttta
     5701 tctataccaa tcagtgtaga aagaactacc atgtaatgat tctagaacac tgatattaat
     5761 aaaacaagca aaaattcatt tttttatgtt tttttaacta tctcagctat ctatcctctt
     5821 tataccatta tttttttcaa atagtgacct gtataacttt cttttacgtt aatgatttct
     5881 tctggtgttc cagtcgcaag gatcgttcct ccaccttctc caccttctgg tcctaaatca
     5941 atgatataat ctgctgtttt gattacatct agattgtgtt caatcactaa aactgtgtta
     6001 ccagcatcta ctaatctttg taatacatgc aacaagcggg cgatgtcatc gctatgaagt
     6061 cctgtcgttg gttcatctag tatatagaaa ttctttccat tagagatttt gtgcaattca
     6121 ctggcaagtt tcatccgttg tgcctcacca ccggacaatg tcgttgctgg ttgccccatc
     6181 gtcacataac ctaagccaac atcaacaatc gtttgcagtt tgcgatgaat ctttggaatg
     6241 tgcttgaaga attctacagc atcctctact gtcatttcca aaatatcagc aatgcttttt
     6301 cctttgtaat gcacttctaa tgtttcagaa ttatatcgtt taccatgaca tacttcacaa
     6361 ggaacataga catcaggcaa aaagtgcatt tcgatcttaa taattccatc cccgcgacaa
     6421 gcttcacaac gaccgccttt tacgttgaaa ctaaaacgcc ctttcttata accccgcatt
     6481 tttgcctcgt tcgtttgagc aaataaccca cggatatcat caaatacact tgtataagtc
     6541 gctggattac ttctcggcgt ccggccgatt gggctttgat cgatatcgat gatcttttcg
     6601 atactttcgt agccggaaat tgtcttgaat ttacctggct tagcagaatt cttatttaat
     6661 ttttgcgcta acgatttctt taagatacta ttgaccaatg tactctttcc agaaccagac
     6721 acacctgtga ctgctacaaa ttctcctaat ggaaattcaa cactaatatt tttcagattg
     6781 ttttctgaag caccagtgat cttgattgct ttcccattac ctttacggcg ttcttttggt
     6841 acagggattg attttttccc tgaaagatat ttccctgtca aagaattttc atcattagct
     6901 acttcttcag gagtgcctgc agccacgatt tgtccgcctt gttctccggc cccaggtccg
     6961 acgtcaatca aataatctgc tgcccacatc gtatcttcat catgttcgac tacaacgagg
     7021 gtgttgccaa gatcacgcat tttctttaat gagccaatca gtcgatcgtt gtcgcgttga
     7081 tgcaatccga tcgatggttc atctaaaata tacaaaactc cagaaagatt cgatccaatt
     7141 tgtgtagcca atctgattcg ctgcgcttct cccccagata atgttccgga agcgcgactc
     7201 aaagttagat agtctagtcc cacattttcc aagaatgaaa gtctgtcatt tacttctttt
     7261 aaaatcggtt tggcaattac tttctcttgt tcagataacg ttaagtttga gaagaaatct
     7321 actgcttttt gaatagacaa ctcatttgtt tgcccgatat tcgttccatt gatttgaaca
     7381 gatagagctt ggggattcaa tcgataacct ttacatgtct gacaagttaa ttctgtcata
     7441 taaagacgca tttgctcacg cgtgaaatca ctgttcgtat catgataacg acgctcgata
     7501 ttcttcaata ccccttcgaa agtcgtttct acatcgcgaa cgccaccaaa atcattttca
     7561 taatggaaat ggaaaagatc atctgtccca ttcaagatga tctcctgatg ctcttccggt
     7621 aattcttcga atggtgtatc taaatcgata ccgaaacttt cacaagcctg cgctaacatt
     7681 tgaggataat actgagagct gattggattc catggaacga tagcaccttg actaagtgtc
     7741 ttcgttctat ctggaatcac taaatcttga tcgacttcta atttgacacc taaaccatca
     7801 cagtctggac aagctccaaa aggcgcattg aatgagaaca gtcttggttc tagttcgcca
     7861 acagtaaatc cgcaataagg acaggcatag tgttcgctaa acaacatttc ttcttcaccg
     7921 atcacgtcaa ccagagcata gccgtctgct aatcgcaggg ccgcctctaa agaatcaaat
     7981 aggcgagaac gaatcccttc tttcacaatg atccggtcga tgacgatcgc gatatcatgt
     8041 tttttgtttt tctccagctc aggcacttca cttaaatcat agatctcacc atcgactcga
     8101 acgcggacgt atccctcttt ttggattcgt tcgaatattt ttttatgttg tccttttttc
     8161 tttaccacga tcggtgccaa cacttggatc tttgtgcgct caggcaaagc cattacttga
     8221 tctgccattt gatctactga ttgactggta atctcgatat gatcgttggg acaaatcgga
     8281 tgtcctacac gtgcaaacaa caagcgtaaa tagtcattga tttccgtcac agtacctact
     8341 gtcgaccgag gatttttact tgttgttttc tgatcaatag aaatcgccgg actcaatccg
     8401 tcgatgctat ctacgtccgg cttatccatt tgtcctaaaa actgacgagc ataagcagaa
     8461 aggctctcta cgtatctccg ctgcccttca gcgtacagcg tatcaaatgc tagggaactt
     8521 tttccagaac cggacaaacc tgtaacgacc acgaatttat ctcgtggaat cgttacatct
     8581 atatttttca agttatgggc acgtgcacca tgaatcgtaa ttttatcgtt agccatgttg
     8641 tcctcctact ttgaagcttt taattctaaa atcatatctc gcacattcgc ggctgtttca
     8701 aaatccaaag cttttgccgc atctttcatt tctttttcta atttcatcaa taacgtgtct
     8761 ttttcttcct tcgtcatttc ttcgtacgag acttgtaccg cttcttttgt atcatcttcc
     8821 gattctttcg taatagaaat caaatctcgg atttctttaa tgatcgtttt tggaacgatc
     8881 ccatgttctt cattgtactt ctgctgaatc gtacgacgtc ttgacgtttc atccatcgct
     8941 agacgcatag aatctgttac tttgtctgcg tacatgatta ctttaccttc ttcattccga
     9001 gcagctcgtc caatcgtttg gactaaggaa cgttcactac gtaaaaagcc ttctttatcc
     9061 gcatccaaga tagcaacaag ggaaacttca ggtacgtcta atccttcacg aagcaaattg
     9121 attccaacta acacatcaaa ttcacctaat cgaagatctc gaatgatttc tgttcgttcc
     9181 agcgttttga tatcactatg caaatatttg actttgatcc ctaattcttt gaaataatct
     9241 gtgaggtctt ctgccatttt tttcgtcaat gtcgttacaa acacccgctg gtctttttcg
     9301 actcgttcat ttatttcgcc tactagatca tcgatttgtc ccatgattgg acgaacttcg
     9361 atcactgggt caagcagccc tgtcggacgg atgatttgtt ggataacggt atctgtttgt
     9421 tcatgttcat atggtcctgg cgtcgcagag acataaatga tctgattgac atgtttttca
     9481 aactcttcta accgaagtgg ccgattgtct aaagcagatg gcaaacggaa accgtaatct
     9541 actagcatct gttttctcgc tcggtcacca ttatacattc ctctgatttg aggcatggtt
     9601 acgtgcgatt cgtccgccac gatcaaaaaa tcttcaggaa agaaatccaa tagcgtataa
     9661 ggcggttcac cttctttacg tccgtccata tgccgagaat aattttcaat tcccgacgtg
     9721 tagcccattt ctaacatcat ttcaatatcg taattggttc gctgttctaa acgttgagct
     9781 tccagaagtt tgttttcatt tcgcaagaca gttaaacgct gttctaattc cgctttgata
     9841 ttcgctacgg catgctccat atgttcatca ttcgtcacaa agtgagtcgc cgggaagatt
     9901 gctacgtgtt ccgtttctcc taaaacttca cctgtcagtg cattgacttc tcggatacgt
     9961 tcgacctcat ctccaaaaaa ctccacacgt aaggcacgtt catctcttga agccggaaaa
    10021 atctccacga catcaccgcg cactcgaaaa cgaccgcgct gaaaatctat atcatttctt
    10081 tcgaattgga tactgactaa gtcacgaatc aactgattgc gatctagttc tgctccctgt
    10141 cgaatcgaaa ctacttgttt ttggtactca aaaggcgagc ctaatccaaa aatacatgaa
    10201 acggatgcaa tgacgatcac atcatttctt tctaataatg aactagtagc cgaatgtcgt
    10261 aatttgtcga tctcatcatt tacacttgaa tctttttcga tatacgtatc gcttgaaggc
    10321 acataagctt caggctgata ataatcataa taagaaacaa aatattcaac agcattgtta
    10381 gggaaaaatt ccttgaattc cccgtacagc tgaccagcca atgttttgtt atgggcaata
    10441 atcagtgttg gtttattcac tttctcaata agattggaaa tcgtatatgt tttccctgtt
    10501 ccagtcgcac ctaataagat ttgtgctttt ttcccgccga ctacgccatc cactaattgg
    10561 ttaatcgctt caggctgatc accagccggt tggtatttcg agacaagctc aaagtgtcgg
    10621 gaggtatctc tctcaatcat tcttcgtcct ccttttcatt cattcctttt cattttacga
    10681 ccaataaaaa gaaaaggaaa gcaaaggcat tccttactaa aaaattatac cacaccgaac
    10741 atactttcgc cactttttta tcttattata catgttatgt tttataacat aaaatgttag
    10801 aaactcgcat ttttttcatt ttttcgagct tacagtcttg tctgattcag cacttttcgc
    10861 ctataataat aaaatattgg aaatttagga ggttgattat gaaaagaaag catttatttc
    10921 actcatttgt tttcttaatg acaggacttg tgacatttgc gtttggtcat gttgcacaag
    10981 ctgacaacac tacaacggca gagtcaagca caagcgagac aatgcaaaaa atagagccca
    11041 aaaaagacgt atacgtaatt gccagtgact ctgcttttgc tccatttgaa tttcaaaacg
    11101 caaagggcga gtacgaaggc atcgatgtaa aattgatgga aaacatcgcc aaactgcaag
    11161 gatttaacat ccgaatggac taccgtggtt tctctgctgc tttgcaagct ttggaatcag
    11221 gacaagcaga cggaatgatt gccggaatga ctgtaacaga tgaacgaaag aaaagctatg
    11281 atttttctga ttcttacttt gatagcggga tccaattagc tgtcaaaaaa ggagatactt
    11341 cgatcaagtc ttatgaagat ttgaaaggaa aaacagtcgg agcaaaagtt ggtaccgaaa
    11401 gtgctgaatt tttaagcgac catgaatctg aatatggcta ttctatcaaa tattttgatg
    11461 ctgctgatca attgtacgac gggttaaaag ttggcgcaat cgatgcaatg atggatgatt
    11521 atccggtaat cggttatgcg atcaaacaag gacaagaatt ggaaacaccg atcaaaaaag
    11581 aatctggcgg aaaatatgca tttgccgtta aaaaaggaca aaatcctgag cttcttaata
    11641 tgttcaatga aggattgaaa gaattaaaaa gaacaggcga atacgataag attattaaag
    11701 aatatgtagc ggatggttca gaaacttcca gtgagtcttc cactgctgat gaatcaacat
    11761 ttcttggact tatccaaaac aactatcaag cattgttgaa aggactttgg agaacaattc
    11821 tcctaacttt gatctccttt gctttggcta tagtaatcgg tgtgattttt ggattattca
    11881 gtgtagcacc aatacgcgct cttcgcacaa ttgctagtat ttatgtcgat atcatccgag
    11941 gaattccaat gatggttctt gcgttcttta ttttctttgg gttgcctggt atcgttggat
    12001 ttacgattcc tgacttcctt gctggtgtta tcaccttgac tcttaacgca agtgcttata
    12061 tcgcagagat tgttcgaggc gggatcaacg ccgttcctgt cggacaaatg gaagcttcca
    12121 gaagtttagg tctttcttat aatcggacaa tgcaaaaaat cattttgccg caagcgatac
    12181 gcatcatgat cccatcattc gtcaatcagt tcgttatctc attaaaagac acaacgatca
    12241 tttccgcaat cggtgtagtc gaattgctgc aaactggtaa gatcattgtt gcacgtacaa
    12301 cacaaagtac gtatgtttac ttgatcattg cgatcatgta cttgattttg atcacagcat
    12361 tgacaaaatt agcaaaagtt ttagaaaaga aggtgaagta agatggcaga aaaaatctta
    12421 gttgagcatt tagtcaaaaa atacggcgat aacacagtat taaacgacat caatgtatcc
    12481 atcaacgaag gagatgtcgt ctgtatcatt ggcccttcag gttcaggaaa aagtacattc
    12541 ttgcgttgct tgaatcgttt agaagaacct tctgacggtg aaattattat tgacggcgcc
    12601 cacttgatgg ataaaagcac agatatcaac ttggtgagac aacatatcgg aatggtattc
    12661 caacacttta acctattccc tcatctttct attttggaaa acatcatttt agcaccgatg
    12721 gacctgaaga aagaatctag agaagaagca gagaaaaaag caatcaaatt actgcagaca
    12781 gttggtctag aagataaaaa agatatgtat cctgaaatgc tttctggtgg acaaaaacag
    12841 cgtgtggcta ttgctcgtgc tctagcgatg aaccctgaca tcatgctttt tgatgagcct
    12901 acttctgcac tcgatcctga aatggtcggt gatgtcttga acgtcatgaa aaagttagca
    12961 gaacaaggta tgacgatggt tatcgttacg cacgaaatgg ggttcgctaa agaagtggcc
    13021 aatcgcgtca tgttcattga cgacggaaac tttttagaag atggaacgcc tcaacaaatc
    13081 tttgaaaatc cgcaaaatga acggacaaaa gatttcttga ataaagtatt aaatatttaa
    13141 taaaaaccaa aaatgcacgg tcgccatgaa gggaccgtgt atttttatct tcttattttc
    13201 tcacctaatc gtatcagccg catcgcttgt tttcctgcac aaacaagcaa tcgctctacc
    13261 tgttccatcg cataagaaag taccatcggt tcattgatga tatcaagtac atagtcgata
    13321 ccaccctcac ttaattgagc ttgatttttc ttctccatat tatgtagttg tgggacaact
    13381 ttctgacaat cagcaattgt gtcaagaacg gatcaatcct ttattggagt tgctcgaaag
    13441 ggtcttctct tattacggac aggctttgct cactgtacat agacaacaaa tcattatcct
    13501 agtcaatcgg atttccagag cctcattgct atcattgctt gataaaatcc agacaaagtt
    13561 taataaaatg tatcagttac agctaaactt tggaattggg tcgctttgtt atacagagca
    13621 agagacaccg caatctttcc ttcatgccaa acaagtttgc gaatggatag ctttccatca
    13681 atctgtgaac gagatccgct tttttgaaga tttagacctc gggatcgttt taccagcaat
    13741 cccgtcagat caacgaacat tatatgtaaa acgtattttg aaaagcctga cagaagaaga
    13801 agtacatttg ttcaagaaga ctttggcatg cttcagtaaa aataacggaa gcatcaaaaa
    13861 ttgctcagaa gaattattta ttcataaaaa caccttgcaa tatcgattga acaagttcca
    13921 ttctctcact ggttataccc ctagaaacta tgacgattat catattctaa agctggcttt
    13981 tttgttggta caaacatgac aactacaaaa aaaccaggcg aacatatgat tgttcgtctg
    14041 gtttt
//
LOCUS       DACDKM010000072        14001 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20834_2016
            SAMN12640370-rid9891663.denovo.072, whole genome shotgun sequence.
ACCESSION   DACDKM010000072 DACDKM010000000
VERSION     DACDKM010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640370
            Sequence Read Archive: SRR10040891
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14001)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14001)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742835.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742835.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:04:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,012
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 29 of 75
            Pseudo Genes (incomplete)         :: 33 of 75
            Pseudo Genes (internal stop)      :: 19 of 75
            Pseudo Genes (multiple problems)  :: 5 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14001
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640370-rid9891663.denovo.072"
                     /isolate="E20834_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(<1..276)
                     /locus_tag="IV334_001927"
     CDS             complement(<1..276)
                     /locus_tag="IV334_001927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP8943989.1"
                     /translation="MKKRKGFNHFFMNLWRYKALVLMAVPGMLWMIFFFYIPVMANVV
                     AFKNFHISADGFLASLRESPWVGWENFKFLFSSDQAFLITKNTILYNV"
     gene            complement(755..1474)
                     /locus_tag="IV334_001928"
     CDS             complement(755..1474)
                     /locus_tag="IV334_001928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HAP8943990.1"
                     /translation="MRLDKFLADMGLGSRKEVKGLIKKGTIEVNGQVVKSDKAQVNEF
                     EDAVTYLGEIITYQKDFYYILHKPAGVISATQDNHDQTVIDLLEDQDYREDLFPVGRL
                     DKDTEGLLILTNDGVFAHQLLSPKKHVEKEYLAEVSGIMTEEDIRLFAEGLIIDGDEQ
                     TLPAKLQIDQVDQETETSIIRLILHEGKFHQVKRMVKAVGKEVLYLKRIRMGKFWLPK
                     DLNKGQYRPMTKEELEQVREK"
     gene            complement(1523..3169)
                     /locus_tag="IV334_001929"
     CDS             complement(1523..3169)
                     /locus_tag="IV334_001929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HAP8943991.1"
                     /translation="MDNQRKPALDQLTPQEKMARGSAWMTASNIISRLLGAVYIIPWY
                     AWMGENAKAANGLFNMGYNIYALFLLISTAGIPAAIAKQTARYNSLNEYGTSRRLFIR
                     ALQMMGGLGLLFALFMYIASPWLAHASGGGEELIPTMRSLSIAVLVFPCMSVIRGYLQ
                     GNQEMMPYALSQIVEQVARIFYMLLATFIIMKVMDGDYVTAVTQSTFAAFIGMLASIL
                     VLLYFLKKHQAYTSAFIHYSENKINIATKELLLDTVKEAIPFIIVGSGVTIFKLVDQF
                     TFMKIMSASTEYSNAQLLDLFSIFSANPDKLTMVVIALATSIASTGLPLITEAVTLKD
                     RVGLAKLTSGNLQLFSFFMFPATFGVMLLAYPLNTLFYTPDSLGSNVLIQASFVGLFL
                     GLYMLVSNMLQGMFENKAAINYLVIGFLVKLLLQYPAIRLFEVYGPLLATMIGFAVSC
                     TLILKRIHEVARFKPSFVWRRTLLIFILTLIMLLASWITKMIFGAFLNEDSKFQSLLL
                     ILVVAGIGALVYGYLALKIRLAERLLGPGIARLRHKLKIK"
     gene            3348..3938
                     /locus_tag="IV334_001930"
     CDS             3348..3938
                     /locus_tag="IV334_001930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HAP8943992.1"
                     /translation="MTQEIKKLPVIFLGLFFITVGLNWFLLPHDIASAGVGAIGHLVE
                     TAFSIQRSVTVWSINLMMLLLAAVLLGKSVFFKTVAGSLLFPVVLEIVPKVEMISSHF
                     FSLISGSLLFSLGVYTLYTVGASNGGITIPPIIFHKFFRLPMPQGLLLTNSLIVFLNY
                     YVFGLLETLFVLLSITVSSLFMKILIRLSPVSRKFE"
     gene            4030..5535
                     /locus_tag="IV334_001931"
     CDS             4030..5535
                     /locus_tag="IV334_001931"
                     /EC_number="6.3.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--L-
                     lysine ligase"
                     /protein_id="HAP8943993.1"
                     /translation="MSFSLEDIRHLLLKENLLKEFVSTRGWHLDVPEKAEFLQLSYDS
                     RKADASTLFFCKGMNFKEEYLDSAIEQGIQYYVSEQPYENSAVGIIVTDIRKAMALLA
                     MAFYDYPQNKLKVIGFTGTKGKTTAAYFTKAILDHTTNKKTALLSTMNTTLDGKTYFK
                     SHLTTPESLDLYRMMSEAVENGMTHLVMEVSSQAYKTQRVYGLTLDVGIFLNISPDHI
                     SPIEHPTFDDYFYCKRQLILNSKTVVLNHESDYFDLLRETADLFQIPTITYGRSEESN
                     YQVIRSDKGTHGFTLSSHEDRLAICDTAYDILLAGGFNQENAASAIIAAALTGASSQD
                     AQDGLKEARVPGRMDQLIQSNGAHVYVDYAHNYLSLKTLLKFAKNEHPDGRVIVVLGS
                     PGNKAISRRHDFGKVLSETADIAFLTADDPAFEDPKKIAEEINEAITNPDLIVHYEMD
                     RPEAIRRALAESAPQDSVVIAGKGVDPYQKINGVDEPYEGDYAIVKRLIEE"
     gene            complement(5619..6971)
                     /gene="celB"
                     /locus_tag="IV334_001932"
     CDS             complement(5619..6971)
                     /gene="celB"
                     /locus_tag="IV334_001932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIC"
                     /protein_id="HAP8943994.1"
                     /translation="MFDFLQKYLMGPMGKIAQLKIVRAVMAAGMASIPFTIVGSMFLV
                     LNILPLPFPFLEGFFNATFFKVSDLYMIVNTMTMGILSIYFAIVFAYELTSIERDEQK
                     LNVNPLTGALLSVFAFFMCIPELIMTDGKISLIASINETETVVNGIRMGSFVERLGTS
                     GIFTAIIMSIIAVELYCMCVKRNWVIKMPDVVPPGVSRSFTALIPTFVIAFVVMIING
                     LLVALGTDIFKMIAIPFGFVTQLTNTWLGVMVIYFLIHALWIVGIHGANIITSFLTPI
                     VLANMAANAQGANYPLAGEFNNSYVTVGGSGATLGLIIFIAFLAKSDQLKVLGKASLV
                     PGLFNINEPIIFGMPIVYNPYLAVPFFLAPMASATLAYFAIRLEIVKPMLAQMPWPSP
                     VGIGAFVGSGGDWKAAVLAVICAIIAFIIWFPFIKFYDNKLLNEEREKAAELASEKLK
                     "
     gene            complement(6971..7489)
                     /locus_tag="IV334_001933"
     CDS             complement(6971..7489)
                     /locus_tag="IV334_001933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8943995.1"
                     /translation="MQDTVKRKIELEKKQLSLKNMYFNRYLIVRYLTAFFFFMNMQWM
                     ILLLSAKSLGSSLPMILILAILPAVGEQVKLYRKHQTNVPWTKWYFLFQSVFNLLVIP
                     VLFTSGFTFLYPFMANNNGGQLFVFILVVSGIFASAFIQYRLKKISLNQDQQYIRIKQ
                     YEKALYLGKENN"
     gene            complement(7505..7831)
                     /locus_tag="IV334_001934"
     CDS             complement(7505..7831)
                     /locus_tag="IV334_001934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIA"
                     /protein_id="HAP8943996.1"
                     /translation="MAEKINSETLQVTSFDIIFHSGNARTLIHEAFRSMRKEMFEEVE
                     EKLRQANEEILHAHQSQTQLLKEYASGTKIEMEIIMVHAQDHLMTTMTLLEVAQEMNY
                     LYQKNK"
     gene            complement(7844..9823)
                     /locus_tag="IV334_001935"
     CDS             complement(7844..9823)
                     /locus_tag="IV334_001935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HAP8943997.1"
                     /translation="MKQKEKLLLRYLIDHKKEYVTSQRLASELLLSDRTIRNYLQRIK
                     ELVEKNGGKIIAKPGYGYQLHILQRLTFDLFLSRQEIVPGYAREPQGFYESEDRQKYI
                     LNQLLLEDAILFMDDLAEELYISRSSLTKDMQEIKERLIPYSLKIVSKHGQGTWIEGE
                     ERNRRHFIMDTFFGNHYGNSLKEYLGQSRFFPDISFEELVIIILDETREAKLKVSDFI
                     IQNLTLHLALGIKRLREGFEIKELGIEQEVSKRVEYQVAQRIVQRIEVIAGVSFPEEE
                     IAYLTLHLMAKSNHKENQNDQELSKELAKVLKRLSQEFHFSLVEDLQLFNGLLDHLSP
                     MMIRLSRGIRIENPLTEEIKKENPKVFDAVKRHFSNMPALKNYTINEDEWAYLALHLM
                     AALEKERAAHKLHALIICATGYGSAQLLKNRVVSEFGKNITVVSVKGYYEINETTLKG
                     IDLIISSIDLSTMFFKIPVLHVSIFLNEDDVRKIRKVVGESIPYHSSDRPIPAFSLQQ
                     KKQIYTEQLSEKFFKTYTYEPKKEEILHDLLQKLSINEEEGYIREMRKQIQQRENMGQ
                     IIFSDTVAVPHPAVPVGIETKVAVALIPSGMQWDQYQEIHFVFLVSPSYVENEGITVV
                     TKAIVRLVDQLKVQQEILAEPIFENFSSKFIKLIE"
     gene            complement(9844..10158)
                     /locus_tag="IV334_001936"
     CDS             complement(9844..10158)
                     /locus_tag="IV334_001936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIB"
                     /protein_id="HAP8943998.1"
                     /translation="MKKALIICAAGMSSSMMASKTTDFFKQKREEVLVDAVSATEGDN
                     MIKNSDFDLFLISPQTTMFLDKFKKLGESVGKPVVSIPFQAYVPIPTGIQKLAEVIEE
                     NI"
     gene            10326..10619
                     /locus_tag="IV334_001937"
     CDS             10326..10619
                     /locus_tag="IV334_001937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HAP8943999.1"
                     /translation="MSITVNILYTSTNGQARKFAEEMVERNIVKAIRAEEGNEKYDYY
                     FPLEDPESLLLIDRWKDEEAIEKHHKSEMMAQINELRKKYKLKMKVERFTDLP"
     gene            complement(10697..11809)
                     /locus_tag="IV334_001938"
     CDS             complement(10697..11809)
                     /locus_tag="IV334_001938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HAP8944000.1"
                     /translation="MTVKNKSKKKGRQQVDFYQSLQLDPFILKQKIREAASKKEKCMY
                     ICSLFLRSLFIVLFAICFIIIITTLFESKHKPYAVVLFCMLMSIRFVDFGYKISHSII
                     GLAIVMFSLLVAPYVQLIKWSVMGMLIHFILLSSILMATASDPKMGNASLYGFSYLFI
                     VYSLPKDSLNKNFFTQTSSLLFLFFCWFSVLLYRKHREKNKDKSLFKEIFLKGMYSQE
                     KIWMLIYAFGISLLIVAGEYVPFQRLMWAGFAFSSIVSSYGLMSIGFKERAVDRIIGS
                     LIGCVLFIGISQFIPFNWVGILGGLALGVCSTYRYKTVFNSFGALAITASLFGVPGAV
                     TIRIFENILGVCLGIMYIGVTEILIRKIREKHGLNH"
     gene            12196..12375
                     /locus_tag="IV334_001939"
     CDS             12196..12375
                     /locus_tag="IV334_001939"
                     /inference="COORDINATES: protein motif:HMM:NF026041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8944001.1"
                     /translation="MPMVIRLKKQRYTCKNCRSHWNAQSYFIRPRHSISNHVRHKITS
                     LLTEKVSLFFISKSC"
     gene            12569..12739
                     /locus_tag="IV334_001940"
     CDS             12569..12739
                     /locus_tag="IV334_001940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016615560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAP8944002.1"
                     /translation="MPRLTNYFLSCTNPKEIEFLVTDMNATYFQLTKHVLPNAKLVID
                     RLLGFSTPFKEA"
     gene            complement(13163..>14001)
                     /locus_tag="IV334_001941"
     CDS             complement(13163..>14001)
                     /locus_tag="IV334_001941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HAP8944003.1"
                     /translation="YQLVETQAPTGYELDATPIEFEIERSQTAVVELTKENRLTPGGV
                     VLTKIDDQSGEVLQGAVFELQDANGTVLQSGLTTGADGKLAIDGLAPGVYQLVETQAP
                     TGYELDATPIEFEIERSQTAVVELTKENRLTPGGVVLTKIDDQSGEVLQGAVFELQDA
                     NGTVLQSGLTTGADGKLAIDGLAPGAYQLVETQAPTGYELDATPVTFKIEKEQEVAIF
                     LTKENRKVADSSDIRGNNPTSSGNKHLPKTGETIISQFILSILGVLLVFISIKFRKKR
                     NI"
BASE COUNT         4374 a         2856 c         2394 g         4377 t
ORIGIN      
        1 tacattatat aaaattgtat ttttagtgat gagaaatgct tgatccgaag aaaataaaaa
       61 tttgaaattt tcccaaccca cccaaggact ttccctcaag ctagccaaga acccatctgc
      121 tgaaatatga aaatttttga atgctacgac attcgccatt acaggtatgt aaaagaaaaa
      181 aatcatccaa agcatacccg gtacagccat caggacaagt gctttataac gccatagatt
      241 cataaaaaaa tgattgaacc cctttcgttt tttcatctaa aaaccccttt cgattcattc
      301 gcaacgtaat tatagcgctt tcaataatga gaataaataa acaaagtata gggaaaatga
      361 aacaaagtat aggagaggtt gtgaaatcag cgttttggcc tgagtattag agaaaaaaac
      421 gggaaaatag ttcctcatat tttgccgttt tttgagctaa taccgagggt cagctgattg
      481 aacaccgttt atcagaggtt gtgaattcag cgttttggcc tgagtattag ggagatcgtg
      541 tccttactat ctaactctga gtcacaagga acaatgggga ctgtaacaaa agtcttgtca
      601 cagtccctta ttccgaacaa acggtgttcc agaagtagct tctacggaaa taagccgaaa
      661 tttcacaaaa aagccatccg aaagtcagac ggacaagtct gcttttcaga tggcgctaca
      721 actactctgc ttattctttt agctatccat tcaattattt ttctctcact tgttctagct
      781 cttctttggt catgggtcga tattgccctt tattcaaatc tttaggcagc caaaattttc
      841 ccatacggat tctttttaaa tataagactt cttttcctac tgctttgacc attcgcttaa
      901 cttggtgaaa ctttccttca tgcaaaatca gacggataat tgaagtttct gtctcttgat
      961 ctacttgatc aatttggagt tttgccggca atgtctgctc atcgccatca ataatcagtc
     1021 cttctgcaaa taaacggata tcttcttctg tcattatacc gctaacctca gctagatatt
     1081 ctttctccac atgtttttta ggtgatagta actgatgggc aaacaccccg tcattcgtta
     1141 aaatcaagag gccctctgtg tctttatcta atcggccgac tggaaaaaga tcttcccgat
     1201 aatcctggtc ttccaataaa tcgattacgg tttgatcatg attatcctga gtagcagaaa
     1261 tgactcctgc aggtttatgc aaaatgtagt aaaaatcttt ttgatacgta atgatttctc
     1321 ctaaataagt aaccgcatct tcgaattcat ttacttgcgc tttatcgctt ttcaccactt
     1381 gtccattgac ttcgatcgtt ccttttttga ttaatccttt gacttctttg cggctgccaa
     1441 gtcccatatc tgctaaaaac ttatctaaac gcatgcgttt cccccatttc aaaagagcat
     1501 caatctagct cttctgattc acttatttta tttttaattt atgtcgtaaa cgcgctattc
     1561 ctggtcctag tagtctttct gccaagcgga ttttcaaagc taaataacca tacactaaag
     1621 cccctatacc tgctactaca agaatcaaca atagagactg gaatttgcta tcttcgttta
     1681 aaaaagcacc aaaaatcatc tttgtgatcc acgaagccaa aagcataatc aaggtaagaa
     1741 taaagatcag caatgtcctg cgccagacaa aacttggttt aaagcgggct acttcatgga
     1801 ttctctttaa gatcaaggta caagacacag caaatccgat cattgtcgcc aataaagggc
     1861 cgtatacttc aaataaacga atggctgggt attgaagaag gagtttcact agaaacccaa
     1921 taaccaaata attaattgcc gccttgtttt caaacattcc ttgcagcatg ttggaaacta
     1981 gcatatatag tcctaggaag agtcctacaa aacttgcttg gatcagtaca ttacttccta
     2041 gagaatccgg tgtataaaat aatgtattta gcggataggc aagtaacata acaccaaatg
     2101 tcgccggaaa cataaaaaat gaaaacaact gtaaatttcc gcttgtcaat tttgccagac
     2161 ctactcgatc tttcaacgta acagcttctg taatcaacgg taacccagta gacgcgatcg
     2221 atgttgccaa tgcaataact accatcgtca acttatctgg attggcacta aaaatcgaaa
     2281 aaagatccag taactgtgca ttggaatact cggtggatgc agacatgatc ttcataaaag
     2341 tgaactgatc gactaatttg aagatcgtta cccctgaacc cacaataata aacggaatcg
     2401 cttctttaac ggtatctaac aacaattcct tcgttgcaat atttattttg ttttcgctgt
     2461 agtgaataaa cgccgatgta tacgcttgat gtttcttcaa aaaatacaaa aggacaagaa
     2521 tacttgctag cataccgata aatgccgcga aagttgattg cgtgaccgcc gtcacatagt
     2581 caccatccat gactttcatg atgatgaatg tagccaatag catataaaaa atacgtgcca
     2641 cctgttctac aatctgcgac agcgcataag gcatcatttc ttgattgcct tgcaaatacc
     2701 ctcgtatcac actcatacaa ggaaaaacca atacagcgat actcaaagaa cgcatcgttg
     2761 gaatcagttc ttctccgcca ccagaagcat gagctagcca aggagaggcg atgtacatga
     2821 acaaagcaaa caacaggcct aacccgccca tcatctgtaa tgcacggata aataatcgtc
     2881 gtgaagtgcc atattcattc aatgaattat agcgtgcagt ttgttttgca atcgcagcag
     2941 gtattcctgc tgtagagatc agtagaaaca atgcatatat attgtatccc atattgaata
     3001 atccattggc agccttagca ttttcgccca tccaagcata ccagggaatg atatagacag
     3061 cgcctagcaa tcttgaaatg atattactgg cggtcatcca agcagagcct cgtgccattt
     3121 tttcttgcgg tgttaattga tctaatgctg gttttctttg attatccatc ggtcagattc
     3181 caactttcta caaaacatta ctatcatttt aattggtaaa acagaaactg gcaatcgatt
     3241 ttatataact tttataagaa caattgtttt tttgaattcg gtttcgtttt tttggtaaag
     3301 taaagaaata tacagccttt tatggctaac atttatagga gatatcaatg acacaagaaa
     3361 taaaaaaact acccgttata tttttaggtc ttttctttat cacagttgga ttgaattggt
     3421 tcttgctccc tcacgatata gcttccgccg gagtgggcgc catcggacat ttagttgaaa
     3481 cggccttttc tatccaacgc tcagttactg tttggagcat caatcttatg atgctgcttc
     3541 tagccgctgt cctattagga aaatcagtat tttttaagac tgtagcggga agtctgcttt
     3601 ttcctgttgt tttagagatt gttccaaaag tggaaatgat ctcctcacat ttcttttcac
     3661 tgatttcagg aagtcttttg ttcagtcttg gggtttacac attgtacaca gtcggggcct
     3721 caaatggcgg tataacgata ccgccgatta ttttccacaa attttttcgg cttccaatgc
     3781 cgcaaggact tttacttacc aatagtctta tcgtcttttt gaattattat gtttttggat
     3841 tgttggagac actattcgtt ctcctatcta ttacagtcag ttcattgttt atgaaaatac
     3901 tcatccgctt gtcccctgtg tcaagaaaat ttgaataaag ctggattttg tcatagcgga
     3961 aacaaacgat atattaaaaa gagtgtgcta taataaacca agaaattgaa taaagcgagg
     4021 gatcaatcaa tgagcttctc tttagaagac atccgtcatt tactattaaa agaaaatcta
     4081 ttaaaagaat ttgtttcaac aagaggctgg catcttgatg taccagaaaa agctgaattt
     4141 cttcagttaa gttatgattc aagaaaagct gatgcttcta ctttgttttt ctgtaaagga
     4201 atgaatttca aagaagaata tttggattca gcaatcgaac agggtattca atattatgta
     4261 tcagaacaac catacgaaaa ttcagcagta ggaatcatcg taacggatat ccgtaaagca
     4321 atggcgcttc ttgctatggc tttttacgac tatccgcaaa ataaattgaa agtgatcgga
     4381 ttcactggaa caaaaggtaa aacaactgct gcgtacttta caaaagcaat cttggatcac
     4441 acaacaaata aaaaaacagc attgctttct actatgaata caacgttaga tggcaaaaca
     4501 tatttcaaat cccatttgac aacgcctgag tcattagatt tatatcgtat gatgtcagaa
     4561 gcagttgaaa atggtatgac tcatctagtg atggaagttt cttcacaagc ttacaaaaca
     4621 caacgcgtat acggcttgac tttagatgtc ggaatcttct tgaatatctc gcctgaccat
     4681 atcagtccga tcgaacatcc gacatttgat gactatttct attgcaagcg acaattgatc
     4741 ttgaattcaa aaacagttgt tttaaatcat gaaagtgact attttgattt acttagggaa
     4801 acagccgatc tattccagat tccaacgatc acttatggtc gttcggaaga aagcaattat
     4861 caagtgatcc gttcagataa aggtactcac ggttttactt tatcttctca tgaagatcgc
     4921 ttagctatct gcgatactgc ttatgatatt ttgctggctg gaggattcaa ccaagaaaat
     4981 gcagccagtg cgatcattgc cgcagcacta acaggtgcct ccagtcaaga tgcacaagac
     5041 ggattaaaag aagctcgtgt accaggccga atggatcaat tgatccaatc caatggagcg
     5101 catgtctatg tggactatgc acataattat ctaagcttga aaacattgct gaaatttgcc
     5161 aaaaatgaac atccagatgg ccgtgtaatt gttgtattag gcagcccagg aaacaaagca
     5221 atctcccgtc gacatgattt tggaaaagtg ctatcagaaa cagctgacat cgcctttttg
     5281 acggcagatg acccagcatt tgaagatccg aaaaaaatcg cggaagaaat caatgaagca
     5341 attactaatc cagatttgat cgttcattat gaaatggatc gtcctgaagc gattcgacgc
     5401 gctttagcag aaagcgctcc gcaagattca gtcgttattg cgggaaaagg cgtggatccc
     5461 tatcaaaaaa tcaatggtgt agatgaacct tatgaagggg attatgcaat cgtgaagcgg
     5521 ttgattgaag aataatggta tttatatact agtggtttcc catttctaag aaaaaaaggg
     5581 actgtgattt tttgtcacag ttcctttttt cttagaaatc attttaactt ttcactagct
     5641 aattctgctg ccttttctct ttcttcattt aataatttat tatcatagaa tttgataaat
     5701 gggaaccaga taatgaatgc gatgattgca caaatgactg ctaacacagc agctttccaa
     5761 tcaccaccac taccaacaaa tgctccgatc ccgactggtg aaggccacgg catctgtgca
     5821 agcatcggtt tgacgatttc cagccgaatg gcaaaataag cgagtgtagc agaagccatc
     5881 ggagctaaga aaaacggaac cgctaaatag ggattgtaga cgattggcat cccgaaaatg
     5941 attggttcat tgatattgaa taatcctggt acaagtgagg cttttcctaa cactttcagc
     6001 tgatcagatt tcgcgaggaa tgcaataaag atgatcaatc ctaaagtcgc acctgatcca
     6061 ccaacggtta cataagaatt gttgaattct cctgccaacg gataatttgc gccttgtgcg
     6121 ttagctgcca tattagctaa tacgatcggg gtcaagaaag aagtgatgat attcgctcca
     6181 tggatcccta cgatccacaa tgcatggatc aagaaataaa tcaccatgac accaagccat
     6241 gtattcgtca actgcgtaac aaacccaaat gggatagcaa tcattttgaa gatatccgtt
     6301 cctagtgcaa ctaataatcc attgatgatc atcaccacaa acgcaatgac aaatgttggg
     6361 atcagagcag tgaaagaacg agaaactcct ggcggaacaa catctggcat ttttatgacc
     6421 caatttctct tgacacacat acaatacaat tcgaccgcaa taatcgacat aataatagct
     6481 gtaaagattc ctgaagttcc taaacgttcg acaaaagagc ccatcctaat accatttact
     6541 acagtttctg tctcattgat agaagcaatc aagctgattt taccatcagt catgatcaat
     6601 tcagggatac acataaagaa tgcaaaaaca gatagtaatg cccccgtgag cggattaaca
     6661 tttaattttt gttcatctcg ttcaattgac gttaattcat aagcaaacac aatggcaaag
     6721 taaatagata aaatccccat cgtcattgta ttgacgatca tgtacaagtc actaactttg
     6781 aaaaaagttg cattaaaaaa tccttctaaa aatgggaacg gtaatggcaa aatatttaaa
     6841 accaaaaaca tcgagcctac aatcgtgaaa ggaatagaag ccatccccgc tgccatgaca
     6901 gcccgaacga ttttaagttg cgcaattttc cccataggtc ccatcaggta tttctgcaaa
     6961 aaatcaaaca ttagttattc tcctttccta aatataacgc tttttcatat tgtttaatac
     7021 ggatatactg ttggtcttgg ttgagtgaaa ttttctttaa acgatactgt ataaatgcac
     7081 tggcaaaaat accgcttaca acaagaatga aaacaaataa ctgtcctcca ttgttatttg
     7141 ccataaaagg atacaagaat gtaaatcctg aggtaaacaa cactggaatc actaataaat
     7201 taaagacact ctggaacaaa aaataccact ttgtccatgg aacgttcgtt tggtgttttc
     7261 gataaagctt tacttgttct cctactgcag gcaaaatagc taaaatcaga atcatcggta
     7321 aactacttcc taaactttta gctgatagaa gcagaatcat ccattgcatg ttcatgaaaa
     7381 agaaaaaggc agtcaggtaa cgtacgatta gataacgatt gaaatacatg tttttcaaac
     7441 taagttgctt tttttctaat tcgattttcc tttttactgt atcctgcaca atgatccttc
     7501 ctttctattt atttttttga tacaaataat tcatttcctg tgcaacttcc aataatgtca
     7561 tcgtcgtcat cagatgatct tgtgcatgga ccatgataat ttccatttct atttttgtcc
     7621 cgctggcata ttctttcaga agttgagttt gtgactgatg cgcatgaaga atctcttcat
     7681 ttgcttgtcg caacttctct tctacttctt caaacatctc tttacgcatc gagcgaaatg
     7741 cttcatgtat cagtgtccgt gcatttccgc tatggaaaat gatatcaaaa gaagtcactt
     7801 gtagtgtttc actgtttatt ttttcagcca tctgttttcc tccttattcg atcagtttga
     7861 taaatttact gctgaaattc tcaaaaatag gttcagctaa gatttcctgc tgtactttta
     7921 gctggtcaac taacctaacg attgctttag tcacgaccgt aattccttca ttttcgacat
     7981 aagaaggcga aactaaaaaa acaaaatgaa tttcttgata ttgatcccac tgcatcccag
     8041 aaggaatcaa tgcaacggct acctttgttt cgattccaac tggaacagcg ggatgtggga
     8101 ctgccactgt atcactgaat atgatttgtc ccatattttc acgttgctgg atctgctttc
     8161 gcatttctct tatatatcct tcttcttcat ttatgctcag cttttgcagc aaatcatgaa
     8221 gaatttcttc ctttttcggt tcgtacgtat aagtcttgaa aaatttttca gacaactgtt
     8281 cggtgtaaat ctgctttttt tgctgtagag aaaatgctgg tatcggtcta tctgaggaat
     8341 gatagggtat agattctcca actacctttc gtattttacg aacatcatct tcattcaaaa
     8401 aaatactgac atgtaaaact ggaattttga aaaacattgt cgaaagatca atagaagaaa
     8461 taatcaggtc gattcctttt aaagttgttt cattgatctc ataatagcct tttacactta
     8521 ccactgtaat atttttaccg aattcactga ctacgcgatt ctttaatagt tgggcactgc
     8581 cgtaacccgt tgcacagata atcaatgcat gtagtttatg cgcagctctt tctttttcca
     8641 atgccgccat gagatgcaaa gccaaatagg cccattcatc ttcattgatg gtatagtttt
     8701 tcaaagccgg catgttgctg aaatgccttt ttactgcgtc aaagactttg ggattttctt
     8761 tctttatctc ctcagtcaat gggttctcaa tgcgaatacc tcggctcaat cgaatcatca
     8821 tcggtgacaa atgatcgagt agtccattga aaagctggag atcctctact aatgaaaaat
     8881 gaaattcctg agacaatctt ttcagaactt tcgctaattc ctttgatagt tcctgatcat
     8941 tttgattttc tttgtgattc gacttagcca tgagatgtaa tgtcaaataa gcaatttctt
     9001 cttcgggaaa cgatacacct gcgattacct caatacgttg aacgatccgc tgcgccactt
     9061 gatactctac tcgcttagaa acttcttgtt cgatgcctaa ttccttgatt tcaaaccctt
     9121 ctcggagccg tttgatcccc aaagccaagt gtaaagtcag attctgtatg ataaaatccg
     9181 aaacttttaa cttagcttct cttgtttcat cgagaatgat aatgactagc tcttcaaaac
     9241 tgatgtctgg gaaaaaacgg ctttgcccta aatattcttt caatgaattt ccataatggt
     9301 ttccaaaaaa agtatccatg ataaaatgcc gtcgatttct ttcttcccct tcgatccatg
     9361 tcccttgacc atgcttagaa acgattttta gtgaatatgg aatcaatcgc tccttgattt
     9421 cttgcatgtc ctttgtcaaa ctggaacgac tgatataaag ctcttctgcc aaatcatcca
     9481 taaaaagaat ggcatcttcc aaaagcaatt ggttcaatat atacttttgt cgatcttcgg
     9541 actcataaaa tccttgcggt tctctagcat atccaggtac tatttcttgc cgactcaaaa
     9601 acaagtcaaa tgtcagtctt tgtaaaatat gcagttggta gccataacct ggtttagcaa
     9661 tgatcttacc tccgtttttt tctactaact ctttgatccg ctgcaagtaa ttacggattg
     9721 tccgatcaga taataacaat tcagaagcta aacgttgact ggtgacatac tccttcttgt
     9781 gatcaatcaa atatctcaac agcaattttt ccttctgttt cattatcatc cactcctatt
     9841 ccatcatatg ttctcttcga tcacctctgc taacttctgg atgcctgtag ggattgggac
     9901 atacgcttgg aaagggatac taacaacagg ctttccaaca gattctccta attttttgaa
     9961 tttatccaaa aacatcgtcg tttgcgggct gatcagaaat aaatcaaaat cactattctt
    10021 aatcatatta tcgccttctg tagctgaaac tgcatctacg agtacctctt ctcttttttg
    10081 cttaaagaaa tccgttgtct ttgaggccat catcgaggat gacattcctg ccgcacaaat
    10141 aatcaatgct tttttcataa atcaatcact ccttttattt ttataaacca agcatatctt
    10201 gtaagcgttt tctattcaaa atatatattt ccgtagcatt gcggaaaaaa tgtgtatcat
    10261 atcttgaata ataatttctg gcaccgtgat atactagtga tagaattttt tcaggagggg
    10321 atttagtgag tatcacagtg aatattttgt atacaagtac aaatggacaa gccagaaaat
    10381 ttgcagaaga aatggtcgaa agaaacattg tcaaagcgat tcgtgcagaa gaaggaaacg
    10441 agaaatatga ctattacttt ccactagaag atccagaatc tcttttactg atcgaccgtt
    10501 ggaaagatga agaagcaatc gaaaagcatc acaaatccga aatgatggct caaataaacg
    10561 aactccgtaa aaagtataaa ttaaaaatga aagtcgaacg tttcactgat ttaccgtaaa
    10621 aagatacaca tggaatgata gcaatttatc atccatgtgt atcttcactt tttccttatt
    10681 ttttattcga tctcatctaa tgattcaaac cgtgtttttc tcttattttt cggataagta
    10741 tttcagtcac cccaatatac atgattccta aacatacccc aaggatattc tcaaaaatgc
    10801 ggatcgttac tgctcctgga accccgaata aactggcagt gattgccaaa gcaccaaaag
    10861 aattaaaaac tgttttatat ctataggtag aacaaacacc taaagctaga ccacctaata
    10921 taccgaccca gttaaaagga ataaattggc tgataccaat aaataaaaca catccaatca
    10981 atgagccaat gattcggtct actgctcgtt ctttgaaacc aatcgacatc aatccatagc
    11041 tagatacaat agaagaaaat gcaaaccctg cccacatcaa tcgctgaaaa gggacatact
    11101 ctcctgcaac gatcaaaaga ctaatgccaa atgcatagat gagcatccaa attttttcct
    11161 gcgaatacat gcctttcaaa aaaatctctt taaacaagct tttgtctttg ttcttttctc
    11221 gatgttttct gtaaaggaga actgaaaacc aacaaaaaaa caaaaataat aagctacttg
    11281 tttgtgtaaa gaaattcttg ttcaacgaat ctttaggtaa cgaataaaca ataaagagat
    11341 atgaaaagcc gtacaaagaa gcattcccca ttttgggatc actagcggtt gccattaata
    11401 tgctgcttag taaaataaaa tgaatcaaca tgcccataac tgaccatttg atcagctgga
    11461 catacggtgc tactaatagt gagaacatga cgattgctaa acctatgatc gaatgggaaa
    11521 tcttataacc aaagtcaaca aaacgaatac tcatcaacat acagaataaa accacagcgt
    11581 atggtttatg cttcgattca aataatgttg taattataat gataaaacaa atggcaaaca
    11641 atacgatgaa taatgatcgt aaaaacaatg aacaaatata catgcacttt tcttttttag
    11701 aagctgcttc tcgtattttt tgttttaaaa taaacggatc tagttgtaga gactgataaa
    11761 aatccacctg ttgtcgtcct ttctttttac ttttattttt tacagtcatc tatgacaggt
    11821 tgtaaatact atggaacatc atatggcgtt gtcattttct tgtcaactat ttttcctatt
    11881 catgttttta taaaaaaaca tatcgttttc ttgtatgatt aaatcaccac aaaataaaca
    11941 actgagagaa cgatatgctt tatgaatggt tctatcaaaa aaattctgag atcaatagac
    12001 aaaaatttga tgattacaga ggtctcttac gaaaccttac agaagaaaaa aacattgatc
    12061 gttgctgcag ttctcttccc tgctccatgt gtctgtagaa attgtggttc tgctgtggta
    12121 gatggaaata ggaaattaat ggtggtgaaa aacaagaaga aggaaaccat tgtccacttt
    12181 gaataatata atcatatgcc aatggttata cgcctaaaaa agcagcgcta tacctgtaaa
    12241 aactgtcgaa gccattggaa cgctcaaagt tattttatcc gtccaaggca ctcgatttca
    12301 aatcatgtca gacataaaat cacttcttta ctgactgaaa aagtgtcgtt attttttatt
    12361 tcgaaaagtt gttaggtatc tttgacaacc gttattcgca cattgaaaga gttgagaagc
    12421 tatttaccaa agtcttccaa aagaattctg tcttgagtgt tgagggttgt gaatttcgtt
    12481 ctcacgtttc cgttgaggat aaactgagtt ttatttccgc ggatggcgag acagaaaaat
    12541 taatcgagaa aatttaccta cacaaaaaat tcctcgatta acaaactatt tccttagctg
    12601 cactaatcct aaagaaatag aattcttagt tacagacatg aacgccacct actttcagct
    12661 caccaaacat gttctaccaa atgcgaaatt ggtgattgat cgattacttg gtttttccac
    12721 gcctttcaag gaggcgtaac ctttattcac gagttggttg aagctttccg agacaaaaga
    12781 cccgacttgt ttttctcctt attgacaaag agccattaaa actagagaaa aaattacaac
    12841 tgatcgctga ttatttgtag aaagaaaatc aaagtattac ccaataaact tgagtcatta
    12901 ggcaaaaaac actgcgaaac tcaagaatga ttttcgcagt gtttttgtat attttttatt
    12961 aactagtatt actacaaagg tttatttttc acttctcaac gcagcacaaa attgtcttta
    13021 cttatttact ggttgggttc aaccttctct actccgatac taatatttgt ttgtataccc
    13081 cttctaaaat cggtagtttc cattcaattg atgggtacta gttgatttta aaaactatgc
    13141 ttattcaatt ttttatttta gctcatatat ttcgtttctt tctaaatttt atactgatga
    13201 aaacaagtag cacacccaat attgacaata taaactggct aatgatggtt tctccagttt
    13261 ttggaagatg cttatttcca ctgcttgttg gattgtttcc tcttatatct gagctatcag
    13321 caacttttct attctctttt gttaaaaaga tcgccacttc ttgttctttt tcgatcttga
    13381 aagttactgg tgtcgcatcc aattcatacc ccgttggcgc ttgagtttct accagctgat
    13441 atgctcctgg tgctaagcca tctatcgcca gttttccatc tgctcctgtg gttagacctg
    13501 actgcagaac ggttccattg gcatcttgta actcaaaaac agctccttgc aggacttctc
    13561 cgctttggtc atcgatcttt gtcaacacga caccgcctgg tgtaagtcga ttttctttcg
    13621 tcaattctac caccgctgtc tgacttcttt cgatctcaaa ctcgattggt gtcgcatcca
    13681 attcataccc cgttggcgct tgggtttcta ccagctgata cactcctggt gctaagccgt
    13741 caatcgccag ttttccatct gctcctgtgg ttagacctga ctgcagaacg gttccattgg
    13801 catcttgtaa ctcaaaaaca gctccttgca ggacttcccc gctttggtca tcgatctttg
    13861 tcaacacgac accgcctggt gtaagtcggt tttctttcgt caattctacc accgctgtct
    13921 gacttctttc gatctcaaac tcgatcggtg tcgcatctaa ttcatacccc gttggcgctt
    13981 gagtttctac cagctgatat g
//
LOCUS       DACDKM010000073        13791 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20834_2016
            SAMN12640370-rid9891663.denovo.073, whole genome shotgun sequence.
ACCESSION   DACDKM010000073 DACDKM010000000
VERSION     DACDKM010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640370
            Sequence Read Archive: SRR10040891
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13791)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13791)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742835.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742835.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:04:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,012
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 29 of 75
            Pseudo Genes (incomplete)         :: 33 of 75
            Pseudo Genes (internal stop)      :: 19 of 75
            Pseudo Genes (multiple problems)  :: 5 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13791
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640370-rid9891663.denovo.073"
                     /isolate="E20834_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(225..728)
                     /locus_tag="IV334_001942"
     CDS             complement(225..728)
                     /locus_tag="IV334_001942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HAP8944004.1"
                     /translation="MRYVSKFLLGIYLFFLLWLILFKLSFNLPQFLTYSYSNVNLVPF
                     STFSFENTTVLRETTYNLIVFFPFGVLLNVNFKRLSFSKKLGIIFLVSFLAELIQFLF
                     GIGVADITDLITNTTGGLIGLWAYQLLNKHLSTNKLDRLAIILGYILFLFLIVAFLFL
                     TRTVRLQ"
     gene            1087..2112
                     /locus_tag="IV334_001943"
     CDS             1087..2112
                     /locus_tag="IV334_001943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HAP8944005.1"
                     /translation="MFKTYFRFNKIQDNPFRIMNLMLLIFIVLWLGYINHDFLISSFS
                     SLGIFTFFYYQNIPMKYLLKRMFLIGCGLLVAFVLGILSTYVLWLEPFAVALVAFSSR
                     FVLRLFHISKPGGLFFAMLSAMGTSMQLPIAQLPIVSLYFFMGVVFALIAAVITKLLD
                     SRPEQTIEKATLKERFHEEPLVIIDSVFYSAALFLSVYVSHGLNLHNPYWLTLSCASI
                     LLAENLDAMKHRQVQYLIGSMGGLCVSAFLSFVPFTQLQTIFLITFLYGIAQFLVARN
                     YAVANIFLNPMALMLSTLIRGAYLISLIEYRFLGIVIGSFIGLGVAWVMTVGLQHYLT
                     VVRQKLE"
     gene            2435..3313
                     /locus_tag="IV334_001944"
     CDS             2435..3313
                     /locus_tag="IV334_001944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8944006.1"
                     /translation="MRFKDMKRGNLMKKISNEIQILAETLTLPKVVIEQMIVQAKNIL
                     DEVFEQYSHELMDPNKAKATAKKIDKVYEEEPFAALAIYLYAASQSWKKIYQPLKIPR
                     TIYLATMNAFTRFIHEHFQVTGSYRFDRGFWIWHYTSGLIFRIGELEYEMTYFPKGYT
                     DLRLEGKQCLSLHIPSDADLSQNKIRDSYESAIRFFELYFPDYHYQVMYTDTWLLSPN
                     LTKWLKKESKICLFAADYRLLSVDEQDDSGVPWIFGRVDAQIHDYPESTSLQRQAKEQ
                     LLAGEHIGSGLGILTS"
     gene            3789..3905
                     /locus_tag="IV334_001945"
     CDS             3789..3905
                     /locus_tag="IV334_001945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002383181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative metal homeostasis protein"
                     /protein_id="HAP8944007.1"
                     /translation="MEKTDISSAYRRLKSPNIKTRKRALKIIKDVKRNSGKR"
     gene            4833..5171
                     /locus_tag="IV334_001946"
     CDS             4833..5171
                     /locus_tag="IV334_001946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8944008.1"
                     /translation="MTQPVSSEEHDNLFRATEEFKKYQENIVPETPLASEPITDIDGN
                     QIGWYIQYEVMVVNNDSIVKNTVGLELTPIEKVSSLLTYIYTDESDETEIILTDYSKI
                     ATDKKYILWM"
     gene            5265..5495
                     /locus_tag="IV334_001947"
     CDS             5265..5495
                     /locus_tag="IV334_001947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8944009.1"
                     /translation="MLVEKGKENIYYVNVAKVREDENEWKEFKSRYSINSTPTFTVYR
                     EGSIEKTVFWTKESGMSLAEVEEFLDYVSMQQ"
     gene            5895..6266
                     /locus_tag="IV334_001948"
     CDS             5895..6266
                     /locus_tag="IV334_001948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cro/Cl family transcriptional regulator"
                     /protein_id="HAP8944010.1"
                     /translation="MRDYVTDKTQEVINQSQSLNELSDKAKEEAVAIISSGISDYIDM
                     SGEINYGDKDKIVRMIKAFIKTFDVKADLKFNDTYLVGKLINILGTEKDTETFISSLS
                     MLFTDKHFSTQLGGEELVEIF"
     gene            complement(6540..7232)
                     /locus_tag="IV334_001949"
     CDS             complement(6540..7232)
                     /locus_tag="IV334_001949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HAP8944011.1"
                     /translation="MKGIIFDFNGTMFLDSHLHEAAWLHMIHDHTKDGLSDEDILKNI
                     HGRTNTEILKHFISEHLTASEIAVLSEEKESYYRMLCLQNENELQLTKGLEATLDQLT
                     DQQIPLTIATATVKENVAFYFDIFDLARWFDWDKIVYDDGSFPGKPQPDIFLKAADKL
                     SLAPADCLVIEDAYSGLLAAKRAGIGTIIAIDPFGKNKTVFEQAQLNKDGTIKDFTGF
                     WTEHLSATSVNS"
     gene            complement(7235..8020)
                     /locus_tag="IV334_001950"
     CDS             complement(7235..8020)
                     /locus_tag="IV334_001950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HAP8944012.1"
                     /translation="MLKKERLLTIVEMVNKKGILTVNEIINQLDVSDMTVRRDLDELE
                     KSGKLLRVHGGAQSLSYTLDQELSHTEKATLQIQEKKEIAATAASLIHEGETIFLGPG
                     TTIELLATHLLNKKIRIITNNYPVFDILAQADGPEILLIGGDFRKNTGAFVGPITNDN
                     LRRLKFTKAFISSNGVHNEAISTYSTEEGEAQQIALNNSRKKYLLVDNKKFNRDDFYV
                     FYNLHDFDLLITDRQISREVQEHYAQYVDLQIADSTENTTIGA"
     gene            8257..9657
                     /gene="lacG"
                     /locus_tag="IV334_001951"
     CDS             8257..9657
                     /gene="lacG"
                     /locus_tag="IV334_001951"
                     /EC_number="3.2.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-galactosidase"
                     /protein_id="HAP8944013.1"
                     /translation="MLKLPEDFIFGGATAAYQVEGATKEGGKGAVAWDDFLEEQGRFS
                     PDPASDFYHQYAKDIELCERFGVNGLRLSIAWSRIFPDGAGKPNPEGIAFYHRVFEEC
                     KKRNVTPFVTLHHFDTPKRLFDHGDFLNRETIEAFVSYAIFCFHEFKEVKVWSTFNEI
                     YPVATNQYLLGVFPPGIKYDFTKIVACLHNMMVAHARVVNYFKENELPGEIGVVHSLE
                     TKYAATDAPEDKHAAFLDDALSIRFLLDATYLGYYSTETLTALDEICEANQASYHFPE
                     EDFVELKKASTRNDYLGINHYQCHFVKAYDGENAIHHNGTGEKGTSVYKVKGIGERIY
                     KEGIPRTDWDWLIYPEGLYDLLLRIKSDYPHYNKIYITENGMGYKDQFEDGIIMDQPR
                     IDYLRVYLESLSKAITAGVNVKGYFLWSLMDLFSWTNGYNKRYGLFYVDFETQKRYPK
                     ESAYWYKLVSETKTII"
     gene            9682..9999
                     /locus_tag="IV334_001952"
     CDS             9682..9999
                     /locus_tag="IV334_001952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010749967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HAP8944014.1"
                     /translation="MNKEELQMLGFEIVAYSGDARSTLLKLLKEVRQGNFEHVDSALK
                     EADENLTLAHNSQTKILAEEASGKEMEMGFIFIHGQDHLMTTLLLRDLIQDFIVLYRQ
                     NQG"
     gene            10012..11685
                     /locus_tag="IV334_001953"
     CDS             10012..11685
                     /locus_tag="IV334_001953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP8944015.1"
                     /translation="MDGIIKQIEKGKPFFEKISRNSYLGAVRDGFLTAMPAILFSSIF
                     ILIAAVPEIFGYQWPESVSTWLWKVYNYSMGVVGLLVSATTARCLADSMNRRLPKNKV
                     INTTSVMLASVVSFLLLSVSPIEGGFSTEYMGTKGLLASFVAAFLTVNLYKFCVLKDI
                     TIKMPKEVPGTISQTFRDIFPFSFSVFAAVLIDLGIRQIFQISFAEAIITVLQPLFTA
                     ADGYLGIAIIWGAMALFWFVGVHGPSIVEPAIAAIIYANVETNLQLFNSGEQASKVLT
                     VGLGNFVGTMGGTGATLVVPFIFLLFAKSKQLKAVGKASFIPVSFAVNEPLLFAAPMI
                     LNPYFFIPFLLTPVVNVCLFKFFVDVLGMNSFMYVLPWATPAPIGLVLGTGMGILAFV
                     LAAVLIVVDFLIYLPFCKAYDAVLVEKEAQKAEVIATEEGASADVREEVPVAASQVIS
                     DANKEINVLVLCAGAGTSAMLANALKKGASESKAPISAMAGAYGSHYEMLQDFDMVVL
                     APQVNSYYDDIKEDTDRLGIKLVATKGAEYIGLTRDPEKAVQFILNQFQ"
     gene            11699..13330
                     /locus_tag="IV334_001954"
     CDS             11699..13330
                     /locus_tag="IV334_001954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016608433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinogalactan endo-1,4-beta-galactosidase"
                     /protein_id="HAP8944016.1"
                     /translation="MKMGIWLRKAVLFAAVLGALGAVSKTVEASASEFIRGADISILS
                     DMEKSGAKYYEDDISKDALEILKNNGVNYVRLRLWQDPYNSNGESYGAGTNDLETTID
                     LAKRAKNLGLKVLLDFHYSDFWVDPGKQNLPKAWQGLTFEEMNTALYDYTKNVLSEMK
                     QLDVYPDMVQIGNELNSGMLWPYGKSWGEGGGEFDRLAAFLKSGIQAVRDTQPKTTPV
                     MLHLADGGDTGAFTWWFDEITSRGVSFDLIGVSYYPYWHGSMNELQTNMNAISQRYGK
                     EVIVVETAYGHTTANADTMPNAFGEAEAAAGGYEPTPTGQAEYLLDLADRIQAVPNNR
                     GAGFFYWEPLWYNGNVSWATQAGMNYLSVQSTMGNEWDNQAMFDFAGNALPSLRAFKQ
                     AGAQINLVKNASFESDGPTTSPSNWQTWFQQGTAKGTIKTEAGTFFGDYKLTFWQDQA
                     YEASAYQNVSGISKGTYTLSAYVMSGGEFDTNQLYIKNYGGAELNTAVVKTSEWTKIT
                     IKDIPITTGVCEIGVYSKASGNAWTNVDFVSLVKQ"
BASE COUNT         4352 a         2243 c         2839 g         4357 t
ORIGIN      
        1 gactaggaag aatgttaaga ttttaagcct ttaagctaaa aaaaaaccct ttttgttaaa
       61 taaggggaat atagcctgtc agtttcaaac tgcaagtcgt cctgctgcaa taatatagta
      121 gattagagat gaaaatagat ttttttaaac caatgactga ataaaaagac cgtcatagat
      181 acacattgga atgtgtctat tacggtcttt ttttacgaac gattttattg aagacggaca
      241 gttcgggtca aaaataaaaa agctacgatc agaaataaga ataaaatgta tcccaaaata
      301 atggctagac ggtctaactt atttgtcgat agatgtttgt ttagaagttg ataggcccat
      361 aaacctatca gtcctcctgt tgtattagtt atcaaatcag tgatgtccgc tacgccaata
      421 ccgaatagga attgaatcaa ttctgccaag aagcttacaa ggaaaataat ccctaatttt
      481 ttagaaaaag aaagtctctt gaaattgaca ttcaaaagca ccccaaaagg aaaaaagaca
      541 atcaagttat aagtggtctc acgtaatacc gtcgtatttt caaaagaaaa agtagaaaaa
      601 gggacgagat tgacattgct gtaagaataa gtgagaaatt gtggaagatt aaatgatagc
      661 ttgaataaaa tcaaccataa tagaaagaat aaataaatcc ctaataaaaa ttttgataca
      721 taccgcatag acatctcctc catttaaaaa gtataccatc gaaaaccgct gaaaaatcat
      781 aaaaaagtat gaagtttaag gaccaacgta gttatcgtac gaaaattaga agcttttttt
      841 tgttgggaat ccggtttctt taacaaagct aagatagcga gttgcttgga aaagttgcta
      901 tcttttttct gtcaaaacga ctcggaatgt tcgttttttg ttctaatttc tccaagttgt
      961 ttactttttg atactaagtt tatgaaaaat caagaaatag ttgttatact aggtaatgct
     1021 gacaaacggg attggcattt catcttggtt cagctttttt tccaattact aggaaggagg
     1081 gatagattgt tcaagactta ttttcgtttc aataaaatac aagataatcc ttttcgtatc
     1141 atgaatctga tgctacttat ctttattgta ttatggttag gttacataaa tcatgatttt
     1201 ttgatttcaa gtttttcatc attagggatc tttacttttt tctattatca aaacataccg
     1261 atgaaatatt tgctgaaacg tatgtttctg atcggttgcg gactgttggt tgctttcgtt
     1321 ttggggatct tatcgactta tgttttatgg cttgaaccat ttgccgtagc tttggttgct
     1381 ttttccagca gattcgtgtt gcgattattc catatttcta aaccaggtgg cttgtttttt
     1441 gcgatgcttt ctgccatggg aacgagcatg cagctgccaa tcgctcaatt accgatcgtc
     1501 tcactttatt tttttatggg tgtcgttttt gccctgattg cggcagtgat tacaaaatta
     1561 ctggattcac ggcctgagca gacaattgaa aaagcaactt taaaagaaag atttcacgaa
     1621 gagcctttag tgatcattga ttctgttttc tacagcgctg ccttatttct ttccgtttat
     1681 gtcagtcacg gcttgaatct gcataatcct tattggttga ccttgtcatg tgcatcgatt
     1741 ttgctagcag aaaacttgga tgcgatgaag catcgacaag tccaatatct gattggatcg
     1801 atgggtgggt tatgtgtgtc ggcattcttg tcatttgtgc cgtttacgca gttgcagacc
     1861 atttttctga tcacttttct atatggaatc gcacaatttc tagttgctag aaattatgca
     1921 gttgctaata tttttcttaa tccgatggcg ttgatgctat ctacacttat tcgtggagca
     1981 tacttgattt ctttgatcga atatcgtttt ttagggatcg tcataggaag tttcatcggt
     2041 ttaggtgttg cgtgggtaat gacggttggt ttgcaacatt atttaacggt tgttcgtcaa
     2101 aaattagaat agaaggacaa atatatgtgc caaaagaggt tatgacaaga tttttgtcac
     2161 aacctctaat tcaaggtgtt caaaagccag attctcagca ttaagtcaaa ttcacacaaa
     2221 aatgtggaaa ggaactttca aaaaaatttt atatttattc tatcaaaaaa ttttgtttat
     2281 gatggtaact gaataaactt tcctgagatg gtcaatgctt tccttgaaag tcttacagat
     2341 attctttctt catagaaagt ttaagtttta tagttagtgt acaataaaag tatgtttata
     2401 ccatatggtg tccggataat cagtcattta aacaatgagg ttcaaagata tgaaaagggg
     2461 aaatttgatg aagaaaataa gcaatgaaat ccaaattctg gcagaaactt taactcttcc
     2521 caaagtcgtt atagagcaaa tgattgtaca agcaaaaaac atccttgatg aggtgtttga
     2581 gcaatatagt catgaactaa tggatcctaa taaagcaaag gcgactgcaa agaaaataga
     2641 caaagtctat gaagaggaac cttttgcagc attagctatt tatctatatg cagcttctca
     2701 aagttggaaa aaaatttatc aaccattgaa gattccaaga actatctatt tggcaacaat
     2761 gaatgctttt actagattta ttcatgaaca tttccaagta acaggaagct atcgctttga
     2821 ccgtggtttt tggatttggc attacactag tggcttgatc tttcggattg gtgaattgga
     2881 gtacgaaatg acatattttc cgaaagggta cacggatcta agattagaag gaaagcagtg
     2941 cctttctctt catattcctt ccgatgcaga tttatctcag aataagataa gagatagtta
     3001 cgaaagtgcc attcgttttt ttgaacttta ttttcctgat taccattacc aagtgatgta
     3061 cacagatact tggttactat cacccaatct tactaaatgg ttgaaaaaag agtcaaaaat
     3121 ctgtcttttt gcagccgatt atcgattatt atcagttgat gaacaggacg atagtggagt
     3181 cccttggatc tttggacgag tagatgccca aatacatgat tatccagaaa gtacgagttt
     3241 acagcgacaa gcaaaagagc aattgcttgc aggcgagcac attggctctg gcttaggtat
     3301 tcttacttca taagtttagt acttcgaaat agtttaaaag agcgtgagac aaaagtaaag
     3361 aacacttttg tctcacgctc taaacacgta taaacggcgg aagcagaagc aactccttcg
     3421 gaaataagct gaaattcaca aaaatttgaa aagcaatttt cgtgaatttc ttcttatttc
     3481 ttggagttaa acgcttctgt cccagcctct tttttagtgt tctatcgaac acacagtacg
     3541 ttcaataggt aaactagcta taacaataga tattaaagtt aacggacaag agggcctttt
     3601 ttaggtagct aaaacgtaat aatgaaaatt tatttccaga gataggagtc aatcagctag
     3661 tctgatcgac tgttttaaca agttgacttt cattttaact tttgatacca tttaaaacac
     3721 aaatcgtaat tgtttcgatt tgtgtttttt agcatgacta caatcaaaga agaaagtggg
     3781 tcgtccatat ggaaaaaaca gatatctcaa gtgcataccg ccgcttgaaa agcccaaata
     3841 tcaaaacacg aaaacgtgcg ctgaagatca tcaaagatgt gaaacggaat agtgggaaaa
     3901 ggtaagaaga aggaaactta attaaaaaaa tagccgaaaa acgtttttcg gctattttca
     3961 ataatcggtt tttgtgtaag gtttccgaaa gaactgctgt tgattttcca tttttaacat
     4021 atgataaatt ggtagaatct ttcttttcaa attgatacca ttccctaatt ccagttatat
     4081 agttaactac agaaccacaa agcacgtacg tttataaaag tattatctgt agttattttt
     4141 caattccctt tcaacctctt attttgacca gtagtattga tcactttacc ttttaacacc
     4201 tttgttcgtt tttgaaacat ttttagtcaa gaatggaaac gtaactacat aatcaaaggt
     4261 tttgcgaaat atttcatgaa atatttcatg tagaagcgag caatacggtt gcaaataacc
     4321 attatcgttt aataagcagt tacgaaagct cccaatatct ctaacgttca gctcactcca
     4381 tttcttttct tcaaaaaaat cagtgattcg tatctctttc tatttgtcga aaaagttaac
     4441 tttaaaaagt aacgccgaaa aaatatgcaa aaaaataaaa aagcagttac tattgctaga
     4501 ataacatcaa ggaataataa gttttggttt tcagataata gtcgataagt tgtgtattgg
     4561 aattttagat acagtaataa agtatttttc atgaaatttt gtgagaatca ctattacaag
     4621 tatctttaat aaaattaatg gttaaggagg aacttaaagt agaatgtaaa tgaatagttg
     4681 tagtcagtcg attgtgatac tagactcaaa gttgcaggaa taagtttttc aatttgtgat
     4741 tggaagataa aaaatgaaaa agaagggctt ttttttatac tttgttgatt gtagtgttta
     4801 ttttccctct gtttagccaa gtaacatttt ctatgactca accagtttca agtgaggaac
     4861 atgataacct ttttcgtgct acagaagagt ttaagaaata tcaggaaaat attgttccgg
     4921 agactccatt agctagtgag ccaattactg atattgatgg aaaccaaata ggatggtata
     4981 tacagtacga agttatggtg gtaaataatg actcaatagt aaaaaataca gtaggcttag
     5041 agctaacacc gattgaaaag gtgagttcat tgttaacgta tatttatact gatgaaagtg
     5101 acgagactga gatcatttta acggattact caaaaattgc tactgataaa aagtatatat
     5161 tgtggatgta gacactagtg aaatacttta tgatatagaa attgatgaaa atttactaga
     5221 tgtagcacaa gaaatcgatc atcaaaagca ggaagctatt cgatatgctg gtagaaaagg
     5281 gaaaagaaaa tatttattac gtgaatgtag caaaagttag agaagatgaa aatgagtgga
     5341 aagaattcaa gagtcgctac tcaattaact cgactccgac atttacagtt tacagagaag
     5401 gatcgataga aaaaactgtt ttttggacta aagaaagtgg aatgtcttta gctgaagtag
     5461 aagagttttt ggattatgtt agtatgcaac agtaagggag ttcttattat gaaaaatttt
     5521 gacacatatt atgttattgt tttttcgata tctttgctgt taatattact aactttaatg
     5581 cagtataaag agtatccaga gtattttatt ttaacttttg tatatttagt aagtagctac
     5641 acacttctat ataaattcag aagttcttct agagtttcta aagtttgttt cttaattagc
     5701 ttattgattc tataatattt ttgatttata taggaaatta aaaatattat agaatcaaca
     5761 ctcagagaac gggttggttt ttaatagatt taataagtac tgataatgaa atttctcctg
     5821 gttgggaaaa cgaagttggt taccctgtag aagaattttt tcaagattac tttgcggagt
     5881 atgccgatag gataatgaga gattacgtaa cagataaaac tcaagaagtc atcaatcaat
     5941 cccagtcatt aaatgaatta tctgataagg cgaaagaaga agcagttgcc attatttctt
     6001 cagggattag tgactatatt gatatgagtg gtgaaatcaa ttatggcgat aaagacaaaa
     6061 ttgtacgcat gataaaagct tttatcaaaa cttttgatgt gaaggcagat cttaagttta
     6121 atgatactta cctagtagga aaattaatta atatccttgg gacagagaaa gatacggaaa
     6181 cgtttatttc ctctttgtct atgttgttta cagataagca tttttcaact cagcttggtg
     6241 gagaggaact agtggaaatc ttttaaccat tacgcccagc attaataaaa gtatatcaaa
     6301 aaagtactcc tgatgatttt taagattggt ttgagaaata atgtgaaaac actattttta
     6361 atgttaatta tagatagaga tgaacgcgtc atatggttaa aagaatgaaa aagtcaaaga
     6421 tacactgcta tatttcacta aggatatagc agtgtatctt tcatgtcgtt tcactggcag
     6481 ttttttataa aaggaggtgt gcatttcagt tgcttttctt tggtagttag ttgaatcgtt
     6541 taagaattaa cggatgtggc agacaggtgc tctgtccaaa aaccagtaaa atctttgatg
     6601 gttccatctt tatttagctg tgcctgttca aatactgttt tgtttttacc gaatggatca
     6661 attgcaataa ttgttccgat ccccgcacgt ttggcagcaa gtaatcctga gtaagcatct
     6721 tcgataacaa gacaatcagc cggagctaag ctcagtttgt ctgccgcttt aagaaaaata
     6781 tccggttgcg gtttcccagg gaaagagccg tcgtcataga caatcttgtc ccagtcaaac
     6841 cagcgggcta aatcgaaaat atcaaaataa aaagcaacat tctctttgac agtagccgtg
     6901 gcaatcgtta atggaatctg ttggtcagtc agctggtcaa gagtcgcttc aagccctttt
     6961 gtcaactgaa gctcgttttc attttgcaag caaagcatac gataatagct ttctttttct
     7021 tctgacaata cggcgatctc agaggcggtc aaatgctctg aaatgaaatg tttcaaaatc
     7081 tccgtattcg tccgaccatg aatatttttt aaaatatcct cgtctgataa accatctttt
     7141 gtatgatcat gtatcatatg cagccaagca gcttcatgca gatgagagtc caaaaacatt
     7201 gttccgttga aatcaaatat aatccctttc atagttacgc tcctatagtg gtattttcgg
     7261 ttgaatcagc aatttgcaga tcgacatatt gtgcatagtg ctcttgaacc tcccgcgaga
     7321 tctggcgatc cgtaatgagc agatcgaaat catgcaaatt ataaaacaca taaaaatcat
     7381 ctcgattgaa ttttttatta tcaacgagta gatatttctt acgggaatta tttagagcga
     7441 tctgctgtgc ttctccttct tcggtactgt aagtagagat cgcttcgttg tgaacgccat
     7501 ttgaactgat aaatgctttt gtaaacttta gtcttcttaa attgtcgttg gtgatcggtc
     7561 cgacaaatgc gccggtattt ttccggaaat caccgccaat cagtagaatt tctggtccat
     7621 ctgcttgagc aagaatatca aaaactggat aattatttgt aatgatccga attttcttgt
     7681 tcagtaaatg cgtcgccaat agttcgattg ttgtacctgg tcctaagaaa atcgtttctc
     7741 cctcgtgaat caaggaggct gctgtggcag caatttcttt tttttcttga atctgcagag
     7801 tggctttttc agtgtgagaa agttcttgat ctaaagtgta ggaaagactt tgagcccctc
     7861 catggacacg cagcagcttg cccgattttt ctaattcatc taaatctctg cgaacggtca
     7921 tatcagaaac atctaattga ttgatgattt cattaacggt aaggattcct tttttattga
     7981 ccatttctac gatagtaagc aatctttctt ttttcaacat tgtcttcacc tctaatttaa
     8041 ggataatcta tcatataaac ggacaacagt caatctaatt cgaacaaaag taaacattta
     8101 gggtggtttt tatgtttgtt acaatagatt ttattgctgt tttctggttt ttatgattat
     8161 ttgtgtttgt ttttgttgac aaatgttttg gatgcgttta caatgcaatc agttagaaag
     8221 agcgaaaact gattatagat ttgaggagag atgtacatgc ttaaattacc agaagatttt
     8281 atttttggcg gagccacagc agcctatcag gttgaaggag ccactaagga aggtggcaaa
     8341 ggcgctgttg cttgggatga ctttttagag gaacaagggc gattcagtcc cgatccggca
     8401 agtgattttt atcatcaata tgcaaaggac attgaattgt gtgaacgttt tggtgtaaat
     8461 ggtctaagat tatccattgc ttggagccgc atttttcctg atggagctgg gaaaccaaat
     8521 ccagaaggaa tcgcttttta tcatcgtgtg ttcgaagaat gtaagaaaag aaacgtaacg
     8581 ccgtttgtga cgttgcatca ttttgatacc cctaaacgtt tgtttgacca tggagatttt
     8641 ttgaatcgag aaacaataga agcatttgtt tcctatgcca ttttttgttt tcatgaattc
     8701 aaagaagtca aggtgtggag tacctttaat gaaatctatc cagttgcaac caatcaatat
     8761 ttgttaggtg ttttcccacc aggaatcaaa tatgatttta caaagatcgt cgcctgtctt
     8821 cataatatga tggttgctca tgcccgtgta gtgaactatt tcaaagaaaa cgaacttccg
     8881 ggtgaaattg gtgtagttca ctcattagag acgaaatatg cggcaacaga cgcgccggaa
     8941 gataagcatg ctgccttttt agacgatgca ttgtcgatcc gtttcttatt agatgctacc
     9001 tatttaggct attattcaac tgaaaccttg actgctcttg atgagatatg tgaagcaaat
     9061 caggccagct atcattttcc tgaagaagat ttcgttgaat tgaaaaaagc atccacgcgt
     9121 aatgactact tagggatcaa tcactaccaa tgccattttg tcaaagctta cgatggagaa
     9181 aatgcaatcc atcataacgg tacgggtgaa aaagggacct ctgtttataa agtgaaggga
     9241 atcggagaac gtatttataa agaaggaatt cctcgaactg attgggactg gctgatttat
     9301 ccagaaggtc tatatgactt gttgctgcga attaaaagcg attacccaca ttacaacaaa
     9361 atctatatca ctgagaacgg gatggggtat aaagatcagt ttgaagatgg cattattatg
     9421 gatcagccga gaatcgacta tttgagagtc tatttggaat ccttaagcaa ggcgataaca
     9481 gccggggtaa atgtgaaggg gtattttttg tggtcgctta tggatctttt ctcatggaca
     9541 aatgggtata acaaaaggta tggcttgttt tatgttgatt ttgaaaccca gaagcgttac
     9601 ccaaaagaat cagcttactg gtacaagttg gtcagtgaaa ccaaaacgat tatttaataa
     9661 gtgataatgg aggaagaaaa gatgaataaa gaggaattac aaatgcttgg ttttgagatc
     9721 gttgcatatt caggcgacgc tcgcagtaca ttattgaaat tattgaaaga agtaagacaa
     9781 ggaaactttg aacatgttga ttcggcgttg aaagaagccg acgaaaattt aacccttgca
     9841 cataattcgc aaacaaaaat tttagcggaa gaagcatctg ggaaagagat ggagatggga
     9901 ttcattttta tccatgggca ggatcattta atgaccactt tattgctgag agatctgatt
     9961 caagacttta ttgtactgta cagacagaat caaggatagg agaagaacgc tatggatgga
    10021 atcattaaac aaatcgaaaa agggaaacca ttttttgaaa aaatctcacg taatagttat
    10081 ttaggagccg ttcgtgacgg atttttaaca gcgatgccag ctattttatt ttcaagcatt
    10141 ttcattttga ttgctgcggt tcctgagatt tttggttacc aatggcctga atctgtctcc
    10201 acatggctat ggaaagtcta taattattcg atgggtgttg tagggttact cgtttcagca
    10261 accactgctc gctgcttggc tgattcgatg aatcgtcgct taccgaaaaa caaagtaatc
    10321 aacaccacct ctgtgatgct ggcctctgtg gtaagtttcc tgttgttaag cgtctctccg
    10381 attgaaggtg gattttcaac ggaatatatg gggacaaagg gactactggc ttcctttgtc
    10441 gctgcatttt taacagttaa tttatataag ttttgtgtgc tgaaagatat taccattaaa
    10501 atgcctaaag aagttcctgg aacgatttcg caaaccttcc gagatatttt tcctttctct
    10561 ttttcagtat ttgcggccgt tttaattgat ttaggcattc gacaaatctt ccaaatctca
    10621 tttgcagaag caatcattac ggtgctgcag ccgctattta cagcagctga cgggtactta
    10681 ggaatcgcta ttatctgggg agcgatggcg ctgttttggt ttgtcggtgt tcatggaccg
    10741 tcgatcgtgg agccggcaat tgcggcaatt atctatgcca atgtggaaac caatctgcaa
    10801 ttgttcaatt ccggtgaaca agcttctaaa gtactgacgg tgggtttagg gaattttgta
    10861 ggaactatgg gaggaactgg ggcaacattg gttgttcctt ttatcttcct actctttgct
    10921 aagtctaagc agttgaaggc agttgggaaa gcttccttta ttccagtaag tttcgctgtc
    10981 aacgaaccat tgctatttgc ggcacccatg attttgaacc cttatttttt tattccattt
    11041 ttgttaacac ctgttgtaaa cgtttgcctg ttcaagtttt ttgtcgatgt attgggcatg
    11101 aacagcttta tgtacgtttt gccttgggca actccagcac cgatcggttt ggttctagga
    11161 acgggcatgg gtatcttagc ttttgttcta gcagcggttc taatcgtcgt tgacttcctg
    11221 atctatctac cattttgcaa agcatacgat gctgtcttag tagagaaaga agcacaaaaa
    11281 gcagaggtga tcgcaaccga ggaaggtgct tctgccgatg tacgtgagga agtacctgtg
    11341 gcagcgagtc aagttatcag cgatgcaaat aaagaaatca atgtgttggt actttgtgct
    11401 ggcgcaggga caagtgcgat gttagctaat gctttaaaaa aaggtgcatc tgaatcgaaa
    11461 gcaccgatca gtgcaatggc gggtgcttat ggttctcatt acgaaatgct gcaggatttt
    11521 gatatggtgg tcttagcacc gcaagtcaat tcttactatg atgatatcaa agaagacacc
    11581 gatcgcttag gaatcaagct ggttgcgaca aaaggagcag aatatatcgg attaacgaga
    11641 gatcctgaaa aagctgttca gtttattctg aaccaatttc aataaaggag gagccataat
    11701 gaaaatggga atttggttgc gtaaagctgt tttatttgca gctgtgttag gggctttggg
    11761 ggctgtttca aaaactgttg aagcatctgc cagcgaattt attcgcggag cagatatttc
    11821 gatcctgtca gatatggaaa aatctggtgc aaaatattat gaagacgata tttcaaaaga
    11881 tgccttagag attttaaaaa ataatggtgt caactatgtt cgactgcgtt tgtggcagga
    11941 tccttataat agtaatggag aaagttatgg agcagggaca aatgatctgg aaacgacgat
    12001 tgaccttgca aaacgagcaa aaaatctggg acttaaggtg ctgctggatt ttcactacag
    12061 tgatttttgg gttgatcctg ggaaacagaa tcttcctaaa gcatggcaag gtctgacctt
    12121 tgaagaaatg aatacggcat tgtacgacta tacaaagaac gttctgtcgg aaatgaagca
    12181 gctggacgtt tatcctgata tggttcagat cggcaatgaa ctgaatagcg gaatgctctg
    12241 gccttatggc aagtcttggg gagaaggcgg gggagaattt gatcgattgg ctgctttttt
    12301 gaagtctggt atccaagctg ttcgagacac tcaacctaaa acaactccag taatgctcca
    12361 cttagctgat ggtggcgata caggagcatt tacttggtgg tttgatgaaa tcacgagtcg
    12421 aggtgtgtca tttgatttaa ttggagtgtc gtattatccg tattggcacg gttcaatgaa
    12481 tgaacttcaa accaatatga atgcgatcag ccaacggtat ggtaaagaag tgatcgtagt
    12541 tgaaacagct tatgggcata caacagcgaa tgccgatact atgccaaatg cttttggtga
    12601 agccgaagca gctgcggggg gatatgaacc aacaccgaca ggacaagcag agtatttgct
    12661 ggatcttgcc gatcggattc aggcagtacc gaataatcgc ggggcgggct tcttctattg
    12721 ggaacctctt tggtacaacg gaaatgtgag ttgggcaaca caagcaggta tgaattatct
    12781 cagtgttcaa tccactatgg gaaacgaatg ggacaaccaa gcgatgtttg attttgctgg
    12841 aaacgccttg ccaagcttgc gtgcgtttaa acaagcaggg gcgcaaataa atctagttaa
    12901 aaatgcaagt ttcgaaagcg atggaccaac gaccagtcct tccaattggc aaacctggtt
    12961 tcaacaagga accgctaagg gtacgatcaa aacggaagcg ggcacttttt ttggggacta
    13021 caaactaact ttttggcagg atcaagcata cgaggcatca gcttatcaaa atgttagtgg
    13081 cataagcaaa ggaacttaca cattgagtgc ctatgtaatg tctgggggag agtttgatac
    13141 taatcagtta tatatcaaaa attatggtgg agcggaacta aatactgcgg ttgttaaaac
    13201 aagtgaatgg acaaaaatta caatcaaaga cattccaatt acaacaggtg tttgtgaaat
    13261 tggggtttat agcaaggctt caggaaatgc ttggacgaac gttgattttg tttcattggt
    13321 gaaacaatag actgcggatc gtttcgattc aagatcggag gttcccttca gccacatcgt
    13381 tagcgtagaa ggcaggaatc gaacctgcac ctcgcctttg ccaaaaataa ccagaaacga
    13441 tttccagtca tttttaccaa aacgagtaaa gcaccagact tcatctacac tttgtattat
    13501 gctaaagaga gttcaacgat ttcgtaagaa gcaatgaaaa aattgcaaaa agttgtgcag
    13561 ataacataaa gtttgtttaa gaaagggtaa ccttagaatt ccgtcgtgaa tatgatttga
    13621 gcacttagat ggatagaaaa aagttctcgg aaaacagttc gtttttcgag aacttctttt
    13681 tatgataaga atgaaacaaa ttaacttata ttatagtctc ttttggtagg gaaccatctt
    13741 atttttaatg aaaatcaagc attttagcta ttgaagaatg ttgatttata g
//
LOCUS       DACDKM010000074        13747 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20834_2016
            SAMN12640370-rid9891663.denovo.074, whole genome shotgun sequence.
ACCESSION   DACDKM010000074 DACDKM010000000
VERSION     DACDKM010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640370
            Sequence Read Archive: SRR10040891
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13747)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13747)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742835.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742835.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:04:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,012
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 29 of 75
            Pseudo Genes (incomplete)         :: 33 of 75
            Pseudo Genes (internal stop)      :: 19 of 75
            Pseudo Genes (multiple problems)  :: 5 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13747
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640370-rid9891663.denovo.074"
                     /isolate="E20834_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            206..544
                     /locus_tag="IV334_001955"
     CDS             206..544
                     /locus_tag="IV334_001955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8944017.1"
                     /translation="MPDYKQKAGYSFCSDKFQLGKFTKLAGEEVSTVRIKECPLQIET
                     LVQRISTRQDFAIVEREIVAIFVEEGILHDPTHINVDKWQPLIYKFREYTTANQSLTL
                     NFRFQEFKES"
     gene            complement(749..1324)
                     /locus_tag="IV334_001956"
     CDS             complement(749..1324)
                     /locus_tag="IV334_001956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF159 family protein"
                     /protein_id="HAP8944018.1"
                     /translation="MCGRYFFDLESELLQNYYREAQTKQPKQAAELAAGEVFPSNLVV
                     TLRKEEENILTPEIMKWGFTGFKKGQLMINARAETVEEKKTFHQPFLQNRCVFPMSGF
                     YEWNEEKDKFMFSNKEKIVYIGGFYRSHQDEKMAESIIMTTEPNRTVAPIHDRMPLVL
                     TEEQIEPWVTDISFARKIITQQMPELVMEKV"
     gene            complement(2056..2637)
                     /locus_tag="IV334_001957"
     CDS             complement(2056..2637)
                     /locus_tag="IV334_001957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAP8944019.1"
                     /translation="MKTKEKILEATKDLIYKKGYHETSIRDILAASDTGKGQFYYYFS
                     SKKEVCLAVIHAHMDVWQTELFEDILNAGKQPEEALEEMLDWIFADHETQITYHGCPV
                     GNLIIELSTVDEEYRLLLLELVNQWVKYTKQNILALSEEKLSDEEADRQAKALIAEIQ
                     GSILLLKVTQDLSLLRQNFQLIKERLLTSQKVL"
     gene            complement(2820..3446)
                     /locus_tag="IV334_001958"
     CDS             complement(2820..3446)
                     /locus_tag="IV334_001958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HAP8944020.1"
                     /translation="MFNNKNTAVVLTDPQVEFLKPAGGGFGLTKDILEKYNTIENMIE
                     MVKQAKEKGYKLFISPHYFYPHDHKWQFGGAGEHMMLEQQMFARKSQYESVEEGSGAD
                     FIEELKPYLDENTIISSPHKIFGPESNDLALQLRKNGIDTVILGGMNGNLCSDSHMRE
                     LVESGFNVVVATDAIGAPGQEAMDAALTNFGFIADQVMTTEEVLEQLS"
     gene            complement(3468..4799)
                     /locus_tag="IV334_001959"
     CDS             complement(3468..4799)
                     /locus_tag="IV334_001959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-containing oxidoreductase"
                     /protein_id="HAP8944021.1"
                     /translation="MMEKYQNIVIGFGKGGKTLAKALASKGESVLVVEKSTRMYGGTC
                     INIGCIPSKSLIFNGERGIDFTEAVARKEKLTGMLRAKNYHMISDEATGTVMDGTARF
                     LSNHQIEVTNNGEKVIVEGERIFINTGSEPIILPIKGLNTSRYLIDSTQAMDQEQLPE
                     KLVIIGAGYIGLEFASMFNEYGSKVVVLDAHSEFLPREDEDIAQMILEDMTNAGIEFH
                     LGVSVDQVADQESTAAVTFTEDGQEVTIQASKVLAATGRKPSTASLGLENTDVKLTDR
                     GAIAVDEYLRTDADNIWAIGDVKGGLQFTYISLDDYRIIMDQLKGENKRTTNNRKAVP
                     YSVFITPTLSNVGLTEKQAKAQGVNYKLFKYMTSGVPKAQVLEDPKGVFKVLVDPETD
                     LILGASIYAEDSHEVINLISLAMNGKLPYTLLRDQIYSHPTMSEALNDVLK"
     gene            complement(4954..5355)
                     /locus_tag="IV334_001960"
     CDS             complement(4954..5355)
                     /locus_tag="IV334_001960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HAP8944022.1"
                     /translation="MKKAYETAMINHGGRNGEVEAPNGSMHMKITPPGIHAEGTNPEQ
                     LFAAGYASCFNGAVQHMIKEAGMTAESTVKARVSLYNLDDGGYQIGVVLEVHIDGISE
                     EEAQKIAEDAHEFCPYSKATRGNIDVEVTIV"
     gene            complement(5441..5647)
                     /locus_tag="IV334_001961"
     CDS             complement(5441..5647)
                     /locus_tag="IV334_001961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8944023.1"
                     /translation="MVTNMVIFLTVLVYGVNFLVYMYLKINHIKDYVEKIAIYFGTNM
                     TLLFTSSIFLFFGKIVEDGILVFE"
     gene            complement(5742..7244)
                     /locus_tag="IV334_001962"
     CDS             complement(5742..7244)
                     /locus_tag="IV334_001962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HAP8944024.1"
                     /translation="MKDMKALQNGSDIRGIALPVEGYEVNLTTDMVKKIGWGLANWIK
                     KKKKSTDPNKKWTIGIGHDSRISGPALKEALIQSLKEQGIHVIDFELATTPAMFMSTQ
                     FEQFSCDAAVMLTASHLPYYYNGIKFFTSEGGAEKEDIAFILSQQQKIKSEQTGSVEK
                     ADLLTPYAQDLVTKIKKGIGTLESKPLNGWKIIVDAGNGAGGFFAEKVLNSLGADSTG
                     SQFLEPDGRFPNHIPNPDNKEAMESIKQAVLANEADLGIIFDTDVDRSAVVSASGTVI
                     NRNNLIAVLSTIVLKEHPGTRIVTNSPTSSHLQRFIEEKGGIQVRYISGYRNVINKAL
                     ELNRLGKDTQLAIETSGHAAFKENYFLDDGAYVIAKILMLLPELKRKGITLDEQIKEL
                     KQPVEVQEIRLKLFSDEPREQGNKILEEMKRLLEEEKGMNFDPENEEGIRYDLEEPYG
                     NGWFLIRLSLHEPLLVLQIENDEFGKNKLALVKLAKLFGHFSDLESLTAE"
     gene            complement(7262..7717)
                     /gene="argR"
                     /locus_tag="IV334_001963"
     CDS             complement(7262..7717)
                     /gene="argR"
                     /locus_tag="IV334_001963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HAP8944025.1"
                     /translation="MKKKQRQALIRQIITEQPIGTQEELLARLHEAGADVTQATISRD
                     IREMKLVKSQNENKIVRYTLFNQPSVSLNEERLRTAIRREVLRIQSVQFMVIVLTERN
                     GADVVTNWLDEVAYPEVVGTMAGVDTFIIICRSEEEAQRFAEKLEKMRE"
     gene            complement(7815..8432)
                     /locus_tag="IV334_001964"
     CDS             complement(7815..8432)
                     /locus_tag="IV334_001964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8944026.1"
                     /translation="MEKIEWRKDKSYSMKKSPQILEVPKKPFFSIRGIGDPNQEDFKR
                     RVACLYAVSYAIRMSPKKNWLIPDYQMYTVYPLEGQWGLQEKYLHEPVMKKEHFSYQL
                     MIRQPDFVTEAIAQEAIQRSSKLPEDLKEQLVFKKMEEGLCAQILHVGPYAEEPATFE
                     KLEAFIAKEGYERASKEHKEIYISDPRKSAPEKMKTILRVKIEKG"
     gene            8653..10026
                     /locus_tag="IV334_001965"
     CDS             8653..10026
                     /locus_tag="IV334_001965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HAP8944027.1"
                     /translation="MTKQKQLLLVPAVIATGMMSFAGVLIETAMNVTFPTLIRQFDLT
                     TAQVQWVTTIYLLMISIIVPLSTYLNRNFSLKKLFLASNLLFLAGIMIDFFSPTFMVL
                     LFGRLLQGAATGIALPLMFHIILTFAPLNRRGAMMGVGTLTTAIAPAIGPTYGGIMTS
                     HFTWNHIFLFLVPVLILSLIIGWFAIPEMAVQKSGYLDWISVLGICLLFSGSLTFLST
                     LGTVSSWIALIIGGIGWFLFYYRSKKVEHPLIRLSILSNKTFRLFLIGFLVYQFLLLG
                     VSFVLPNFLQIVQGSSPFVAGLAMLPGAAVGALLSPFSGKMLDQYGPKKPIFAGLILS
                     LIGWLALFLLIGHASVGVLIACHVFYMIGIGLSYSNMMTTGMNALNEELQGDGNAMFN
                     TLQQFSGAVATSLVAVIINYVQHHTSHNYEVSTTLGSKAALGVLLLLLLVSFARFAYY
                     LFFAKKA"
     gene            10101..11213
                     /locus_tag="IV334_001966"
     CDS             10101..11213
                     /locus_tag="IV334_001966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HAP8944028.1"
                     /translation="MEKIAIIGGGIIGMTLASYLDTSKFNVALFDEGTGQATKASAGI
                     ISPWLSKRRNKQWYRLAKDGAAFFPKLIKDFQLDQTIYAQSGTLLLRPEKDLAELAVL
                     AEKRKEEAPEIGNIEWLSPEQTSQALPLLKPAVSLRISGGGRLDGKAYLEHLGQRLAQ
                     KQMEQIQAKVTLHKKSDSWLIKGPFGEYTADRVILTPGPALKPLLQELGYAADIRPQK
                     GQLLAFQTSYKMSQDWPVAMLDGEADFIPFMDGKILLGATHENDQGWDLAPTSEAYKG
                     LTKSTEQFLTDPDQIKNWPYEFRVGTRAYTSDFSPFFGVLPDDPSLLAASGLGSSGLT
                     TGPYIGYLLANFLNTGHWSTEDYQKDISTYITKNPS"
     gene            complement(11285..12922)
                     /locus_tag="IV334_001967"
     CDS             complement(11285..12922)
                     /locus_tag="IV334_001967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HAP8944029.1"
                     /translation="MSTKESLELNEITSGNPLLSSIRSIVETTFYGNNVHEVFDRKTA
                     YQLAKGSPGTIITDLTISHAEELDLPADVRTLVFNDGSIVGRTASARRIFEDLDKEQS
                     KYEKILREAVYQSRKRQFYHTKVIVGLSEEFSVQSHLLIPEGFENNLYSYLLNFQPAN
                     SKILETYQKSVSFDEGDLYIFCDPEWTHETFPNGLILFDALHNTAAVLGLRYFGELKK
                     ATLTLAWALGNRNGFIACHGGMKQYASNDQTYTMAVFGLSGSGKSTITLAKHTEGHSV
                     SILHDDAFVISRKNGFATALEPSYFDKTQDYPSDDPAISSFLTCQNVGVTCTSKGEKV
                     LVTGDIRNGNGRTVKSRYATPDRVDHLSEKIDAVFWIMKDDSLPPIIKINDPVLAASF
                     GATLATKRSDAENITTKRQLDELVIEPFANPFRVYPLREDYQAFRELFLKQQTACYVL
                     NTGYFDDKKIKPETTLSIVERIVQQTAEFKSFGPFEDIHYLPIPDFPVSFEDTSYKNL
                     VKQRLDIRLRFIEAQIGKDLLPDEAKESLENLIQKLN"
     gene            complement(13090..13698)
                     /locus_tag="IV334_001968"
     CDS             complement(13090..13698)
                     /locus_tag="IV334_001968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HAP8944030.1"
                     /translation="MNSFKKIVLGTTFAAGATAMFVGTTNAHADEVYTVKSGDSLSKI
                     SQKFAGDNSMIDAIAEKNSIANINRIYVGEQLTIPTSNDSSATTENKTTENTASTTET
                     ATQEHTYVAPVETVEVAPAAPAAATAPTSSSAKEWIAQKESGGSYTATNGRYIGRYQL
                     DASYLNGDYSAANQERVAEQYVTSRYGSWDAAKTFWLANGWY"
BASE COUNT         3708 a         2891 c         2463 g         4685 t
ORIGIN      
        1 cctttggaag tatttttgag ttacgtaagt attgatgatc tgtctaactt aatttgacaa
       61 ttaagaaact aaaaaaataa tccaagaaac attgaaccta tggatccaaa agctacccaa
      121 atactatagc taaccccccg ctaccttcaa ttttgcagat aaaacgcttt gggagaaaat
      181 ttcaaagaca acaggccatc ctatcatgcc cgattataaa caaaaagctg gctattcctt
      241 ttgctctgac aaatttcaat tgggaaagtt cacgaaacta gcaggtgaag aagtttcaac
      301 tgttcggatc aaagaatgcc cgcttcaaat agaaacattg gttcaacgaa tttcaactag
      361 acaagacttt gctattgtag aacgtgagat cgtagctatt tttgtagaag aagggatttt
      421 acatgatcca actcatatca atgtggataa atggcaacct cttatctata aattcagaga
      481 atatacaact gccaaccagt cgttaacttt gaattttaga ttccaagaat tcaaagagtc
      541 ataatgataa acatcgttag gtataaataa aaaagactcg cagcacgagt aaaaaacaaa
      601 tagaagctat acattagaaa ggtcgactgt gaccctatag atgcagtata tgcagcttat
      661 gagcatttct tctgttctct ttgtttttgg gatctgtgct gaagtctttt tcgttttttg
      721 ccgctctttc tttgaaaaat cgattatgtt ataccttctc cattactaat tcaggcatct
      781 gctgagtaat gatttttcgc gcaaaagaaa tatctgttac ccaaggttcg atttgttctt
      841 cagtcaagac aagcggcatt cggtcatgga ttggtgcgac tgtgcggtta ggttcagtag
      901 tcattatgat tgattctgcc attttttcat cctgatggct acgataaaaa ccgccaatat
      961 acactatttt ttccttattt gaaaacataa atttgtcttt ttcttcattc cactcataaa
     1021 agccactcat tgggaaaaca caacgatttt gcaaaaatgg ttgatgaaag gtcttttttt
     1081 cttctactgt ctctgcacgg gcattaatca tcaattgtcc ttttttaaat ccggtaaatc
     1141 cccacttcat gatttccgga gtgaggatgt tttcttcctc ttttcttaac gtcacaacaa
     1201 gatttgacgg aaaaacttct ccagctgcta attctgctgc ctgcttcggt tgtttcgtct
     1261 gggcttctcg ataataattc tgaaggagct ctgactctag atcaaaaaaa taacgaccac
     1321 acatcctaat ccctcccaat ctttttttct atcatacact gcaacaagaa gttcgcacca
     1381 acaaaacgct ttcaattttt gtagaactaa aaaagttgtg acaagtgaca aactctccag
     1441 tgaatggtat tctcctgcca actcctcggt cacaagtcac aatattcaac aaacatgaga
     1501 agctgctttt tgaaattgtt ccttgtgact cggagtgaaa cggcaggctt acgacctctg
     1561 ggtaaacggt gttcaaaagc cagtttctcg gtattaagcc aaatttatgc aaaaatgtag
     1621 aaagccattt tcacaaaatt tttcttaata ctcgaaacta agcgactttt tcacaacctc
     1681 ttccttaaac ggtgttcaaa agcttgcatc tgcggtaata agcccaacca aacgaaaaat
     1741 atgagcagct atttttattt ggctgttctt attacttgat gcagggcagc tttttcacaa
     1801 cctctccact aaacggtgtt caatcagcta accttcggta ttagctcaaa aaacggcaaa
     1861 atatgaggaa ctattttgcc gtttgtttct ctaatactca ggtcaaagcg ctgatttcac
     1921 aacctctaat acacggtgtt ccagaagtag cttcttcgaa aaataagccg aaatttcaca
     1981 aaaatttgag aagcaatttt cgtaaattcc ttcttatttc ttgaagctga ccacttctgt
     2041 cacaacctct aattattaaa gaaccttttg cgaagttaac aatctttctt tgatcaattg
     2101 aaaattctgc ctaagtagtg atagatcctg tgtgactttc aacaataaga tacttccctg
     2161 gatttcagca atcaacgctt tggcttgacg gtctgcttct tcatcggaca gtttttcttc
     2221 agaaagtgcc aggatatttt gtttcgtata cttgacccat tgattgacta attctagtag
     2281 caacagacgg tattcttcat ccactgtact caactcaata atcaaattac ctacaggaca
     2341 gccatgataa gtgatctgtg tctcatgatc agcgaaaatc cagtccaaca tttcttctaa
     2401 tgcttcttct ggctgttttc cagcatttaa gatgtcttcg aacaattctg tctgccatac
     2461 gtccatatgc gcatggatca ccgctagaca aacttctttt tttgaagaaa agtaataata
     2521 aaattgacct tttcctgtat cagaagctgc tagaatatct cggattgacg tttcatgata
     2581 gcctttttta taaataagat ctttggtcgc ttccaagatc ttttcctttg ttttcaatga
     2641 tattctcctt tttcatttct catagtccta tattatacag taaattcatc attttttctt
     2701 cttaatcgtt taaaaaagac tgtgccaaat tatctgacac agccctcact ataaaactgt
     2761 tctcatatag ctagatgttc attgattcaa gaactgcgag gacaaaagca attcctatct
     2821 tatgatagtt gctctaatac ttcctctgtt gtcatcactt gatctgcaat aaagccaaag
     2881 ttcgtcaacg ctgcatccat tgcttcttgg ccaggagcac caattgcatc tgtcgctacc
     2941 acaacattga atccactctc taccaattca cgcatatggg aatcgctaca caagtttccg
     3001 ttcattccac caagaattac tgtgtcgatt ccattttttc tcaattggag cgccaaatca
     3061 ttgctttctg gtccaaagat tttatgagga gagctgatga tcgtattttc atccaaataa
     3121 ggtttcaatt cttcgataaa atcggctcct gaaccttctt ccactgattc atattgactt
     3181 tttctagcaa acatctgctg ctcaagcatc atatgttctc ctgcaccgcc aaattgccat
     3241 ttatggtcat gtgggtaaaa ataatgagga gaaataaaga gtttgtatcc tttttctttt
     3301 gcttgtttta ccatttcaat catattttcg atcgtattgt atttttctaa gatatctttt
     3361 gtcaaaccaa agccaccgcc tgctggtttc aagaactcga cttgcgggtc agtgagaact
     3421 actgctgtat ttttattatt gaacatggtg gattctccat tcgttattta ttttaataca
     3481 tcatttagag cttcactcat tgtcggatga gaatagattt gatctctcaa taacgtataa
     3541 ggaagttttc cattcatagc tagcgaaatc aagttgatga cttcatgaga atcttccgca
     3601 tagatacttg cccctagaat caagtcagtt tcagggtcga ctaaaacttt gaatacacct
     3661 ttaggatctt ctaatacttg tgctttaggg acaccgcttg tcatatactt gaataatttg
     3721 taattgacgc cttgtgcttt ggcttgtttt tctgttaaac ctacattgga taaagtcgga
     3781 gtgataaata cactgtaagg cacagctttc cggttattcg ttgttcgttt attctcgccc
     3841 ttcaactgat ccataatgat tcgataatca tctagagaga tataagtgaa ctgcaatccg
     3901 cctttgacgt ctccaatcgc ccaaatgttg tctgcatctg tacgaaggta ttcatccaca
     3961 gcgattgctc cgcgatctgt tagtttaaca tctgtatttt ctaatccaag tgatgctgta
     4021 cttggttttc ttccagttgc agccaaaact tttgacgctt ggattgttac ttcttggcca
     4081 tcttctgtaa aggtgacggc agctgttgat tcttggtctg ctacttgatc tacagataca
     4141 cctagatgga attcgattcc cgcatttgtc atgtcttcta aaatcatttg cgcgatatct
     4201 tcatcttcac gtggaagaaa ttcagaatgg gcatcaagaa cgacaacttt agatccgtat
     4261 tcattgaaca ttgatgcaaa ttccaagcca atatatccag ccccgatgat cactaatttt
     4321 tccggtaatt gttcctgatc catcgcttgc gtagaatcaa tcaagtagcg acttgtattc
     4381 aatcctttga ttggtaaaat gattggttca gaaccagtat tgatgaaaat ccgttcgcct
     4441 tcaacaatca ctttttcacc attattcgtc acttcgattt gatggttaga aagaaaacgt
     4501 gctgtaccgt ccatcactgt cccagttgcc tcatcgctga tcatatgata atttttggcg
     4561 cgaagcatgc ccgtcaattt ttctttccga gcaacagcct ctgtaaagtc gattcctctt
     4621 tctccattga aaatcaatga tttagaagga atacagccga tgttgataca tgttccgcca
     4681 tacatacgtg ttgacttttc aacgactaaa accgattcac cttttgaagc taacgccttc
     4741 gctaaagtct taccgccctt tccaaaaccg atcacaatat tttgatattt ttccatcatt
     4801 tttccctcgc ttctttttta cctaccgatt ggtacaaaaa tatgataacc gataattgtc
     4861 tattctccaa atcatctgct ttatttgtca aaaaaaacca gacaaaaagc ggtcctttca
     4921 cgtttttagt ctggttcgat ttcctttatt cttctaaaca atcgttactt ctacatcgat
     4981 atttcctcta gttgctttgg agtaagggca gaattcatgc gcatcttctg caattttttg
     5041 ggcttcttct tcgctgatgc catcgatatg cacttctaat actacaccga tctgatagcc
     5101 gccatcatct aaattataaa gagagacacg tgcttttact gtggactctg ctgtcattcc
     5161 tgcttctttg atcatatgtt gtaccgcccc attgaaacaa gaagcataac ctgctgcaaa
     5221 taattgttct ggatttgttc cttctgcatg gattcctgga ggagtgattt tcatatgcat
     5281 cgagccattt ggtgcctcga cttctccatt gcgcccgcca tggttgatca ttgctgtttc
     5341 gtatgctttt ttcattgtac tgctcctttc gattgaccat ttgctacttg gctataatta
     5401 tagtgtatga caagtaaggc gctttctaca aaaattctgc ttattcgaat accagaatgc
     5461 cgtcttctac tatttttcca aaaaacaaaa aaatactact cgtgaacaac agcgtcatat
     5521 ttgttccaaa ataaatagca atcttttcta cataatcttt tatatgattg attttaagat
     5581 acatatatac cagaaagttg accccataaa ccaatactgt taaaaaaata accatattcg
     5641 ttaccatttt ttcctccttt tgctggtctc cctaccagca ctcgatttaa ccataccact
     5701 ttttttgccg tccaagcaac tagcctttca aaaagactgg tttattctgc tgttaatgat
     5761 tcaagatcag aaaaatgtcc gaataatttt gctaatttta ctaacgccaa cttgttcttt
     5821 ccgaattcat cattttctat ctgtaatact agtaacggct catgtaagct taaacggatc
     5881 aaaaaccagc cgtttccgta tggctcttct agatcatatc gaataccttc ttcattttcc
     5941 gggtcgaagt tcatcccttt ttcttcttcc aacagtcgct tcatttcttc taatatcttg
     6001 tttccttgtt cgcgtggttc atcgctgaac aattttaatc ggatttcttg tacttctacg
     6061 ggctgtttca actctttgat ttgttcatct aatgtgatac cttttctttt taattctggc
     6121 aaaagcatca ggatctttgc gatgacatat gcaccatcat ccagaaaata gttttccttg
     6181 aatgcagcgt gtccgcttgt ttcgatggct agctgtgtat ctttcccaag acggttaagt
     6241 tctaatgctt tattgattac atttcgatag ccggaaatat aacgaacttg gatgcctcct
     6301 ttttcttcaa tgaatctttg tagatgagaa gaagtagggg aattcgtcac gattcttgtt
     6361 cctggatgct cttttaatac gattgtactt aatactgcga tcaaattatt tcgattgatc
     6421 actgttccag aagcactgac aactgcagaa cgatccacat ctgtatcgaa aatgatccca
     6481 agatctgctt catttgctaa aacagcctgc ttgatacttt ccatcgcttc tttattatca
     6541 ggatttggga tatgattagg aaatcggcca tctggttcca aaaattggga acctgttgaa
     6601 tccgcaccta agctattcaa tactttttca gcaaagaatc caccagcacc atttccggca
     6661 tcaacaatga ttttccaacc atttagtggt ttgctttcta gtgttccaat ccccttttta
     6721 atttttgtga ctaaatcttg cgcataaggt gtcaaaaggt ctgctttttc tacgcttcca
     6781 gtttgttcgc tttttatttt ttgctgctgt gataggataa aagcaatatc ttctttttct
     6841 gctcctcctt ctgaggtaaa gaattttatt ccattataat aatagggaag atggctagca
     6901 gtcagcataa ccgcagcatc gcacgagaac tgttcaaatt gagtggacat aaacatagca
     6961 ggcgtagtag ctaattcgaa atcgatgaca tgtatccctt gttctttcag actttggatc
     7021 aacgcctctt tcagtgctgg tccgcttatc ctactgtcat gaccaatccc aatcgtccat
     7081 tttttatttg gatcggttga tttcttcttt ttctttatcc agtttgctag tccccagccg
     7141 atctttttta ccatgtctgt cgtcaagttt acttcatatc cttcaactgg taatgcgatt
     7201 cctcgaatat ccgatccatt ttgcagcgct ttcatatctt tcatataaaa gtcctcctca
     7261 tttattccct cattttttcc agcttttctg caaatcgctg tgcttcttct tctgagcggc
     7321 aaataataat gaatgtatct acaccggcca tcgttccgac aacttcaggg taagccactt
     7381 catctagcca attcgttacc acatcggcac catttcgttc agtcaatacg ataaccatga
     7441 actgaacaga ctggatacgc aaaacctcgc gtctaattgc tgtacgcaaa cgttcttcat
     7501 ttaaagaaac agatggctgg ttaaataatg tatagcgtac tattttgttt tcattttgac
     7561 tttttactaa cttcatttct cgaatgtctc gagaaatcgt tgcttgagtc acatcggcgc
     7621 ctgcttcatg cagccgcgca agcagctctt cctgtgtccc aataggttgt tctgtaatga
     7681 tctggcggat cagtgcttgt cgctgtttct ttttcatatt agacacctct caatgttgat
     7741 tatagcaaaa gataagtaat atttcttcat attttggtat tttttacttt tattttttgt
     7801 tttcattcag gttgctaacc tttttctatt ttaacacgca aaatcgtctt cattttttca
     7861 ggtgctgatt ttcttggatc gctgatgtaa atctctttgt gttctttaga agctctttca
     7921 tagccttctt ttgcgatgaa tgcttctaat ttctcaaacg ttgctggttc ttcggcatag
     7981 ggcccgacgt gtaagatttg ggcacaaaga ccttcttcca tctttttgaa tactagttgt
     8041 tctttcaagt cttccggaag tttgctagaa cgctgaatgg cttcttgtgc tatcgcttct
     8101 gtaacaaagt caggctgtcg gatcatcaat tgataagaaa aatgttcttt tttcatcact
     8161 ggttcatgca aatacttttc ttgcagtccc cattgtcctt ccagtggata gacagtatac
     8221 atctgataat caggaatcag ccaatttttt ttaggcgaca tgcgtattgc atagctgact
     8281 gcatatagac aggctactct tcgtttaaag tcttcctgat tcgggtcgcc gatccctctg
     8341 atgctaaaga atggtttttt aggaacctct agtatttgcg ggcttttttt catactatag
     8401 ctcttgtctt tacgccattc aattttttcc atttttcttc ctccgcgaaa ttccatttag
     8461 ttatttatta tcatacctct ttttgagtga cttggctaat cgtgttgaca ctatttttca
     8521 gtttgtgcta gaataagtga agtaaataaa tcgtacagat aacacgtttc ggcgtgtcga
     8581 gagagaggcg gtttctattt ttataataga aatcactctc tttttttacg aaatttttta
     8641 gaggtgatgt aaatgacaaa acaaaaacag ctgcttttag tacctgcagt aattgcaaca
     8701 gggatgatgt cttttgcggg cgtattgatc gagacggcca tgaatgtaac tttcccaact
     8761 cttatcagac agtttgattt aacgactgca caagtccaat gggtaacaac gatttatctt
     8821 ttgatgattt cgatcattgt tccgctgtct acttatttga atcgcaattt tagtctcaaa
     8881 aaactctttt tagcttcaaa tctgcttttc ttggcaggta taatgatcga tttcttctct
     8941 cctaccttca tggtcctatt gtttggtcga ttgctccaag gtgcagcgac aggtatcgct
     9001 cttccgttga tgttccatat tatcttgaca tttgcccccc tcaatcgacg aggcgcgatg
     9061 atgggtgtcg gtactttgac aacggccatt gctccagcaa ttggccccac ttatggaggc
     9121 attatgactt cacattttac atggaatcat atcttcctgt ttcttgtgcc cgttttgatt
     9181 ctttctttga ttatcggctg gtttgctatt cccgagatgg cagtacaaaa aagtggttat
     9241 ttggattgga taagcgtttt aggaatttgt cttttgttta gtggaagtct aacatttttg
     9301 agtacgttgg gaacagtttc cagctggatt gctttaatca tcggcggtat tggctggttc
     9361 cttttttact atcgaagtaa aaaagtggag catccactga tccgactgtc tattctttca
     9421 aataaaacat tccgcttgtt tttgattggc ttcttagttt atcagttttt attacttggt
     9481 gtatcttttg tcttgccgaa tttccttcag atcgttcaag gaagtagtcc ttttgtcgct
     9541 ggattagcga tgcttcccgg cgcagcggtt ggtgctttgc tttctccttt ttccgggaaa
     9601 atgctggatc aatatggtcc gaagaagcca atttttgctg ggctgattct ttctttgatt
     9661 ggttggctag cattattcct actcatagga cacgcttcag tgggtgtatt gattgcttgt
     9721 catgtgtttt atatgatcgg tatcggatta tcttacagca atatgatgac caccggaatg
     9781 aatgcactga atgaagaact acaaggagac gggaacgcaa tgttcaatac cttgcagcag
     9841 ttttctggcg ctgtggctac ttcattagta gcggtcatta tcaattacgt ccagcatcat
     9901 acttcccata actatgaagt gtctactaca ttaggatcaa aagcagcatt aggcgtcttg
     9961 ttgctgttgc ttctcgtaag ctttgctcga ttcgcctatt atctgttctt tgctaaaaaa
    10021 gcgtagattc catatacggc aggtgttgca tctgccgttt ttttgtgtga aaatggttgt
    10081 ttgtaaaagg aggctttttt atggaaaaaa ttgccattat cggtggcggg atcattggta
    10141 tgactctagc cagttactta gatacatcaa aattcaatgt tgctttattt gatgaaggga
    10201 caggacaggc aactaaagct agcgctggga ttatctcccc ttggttgtcg aaacgcagaa
    10261 acaaacaatg gtatcgtctg gctaaagacg gcgctgcttt tttccctaaa cttataaaag
    10321 atttccaact tgatcaaacg atttacgcac aatcagggac acttttgctt cgacctgaaa
    10381 aagatttagc tgaattggct gttcttgccg agaaacgaaa agaagaagcg ccagaaattg
    10441 gaaacatcga atggctttca cctgaacaga catctcaagc acttccgtta ttgaaaccag
    10501 cagtttcact gcgtatcagt ggtggtggtc gtctggatgg aaaagcatat ttagaacatc
    10561 ttggtcagcg tttagcacaa aaacaaatgg agcagatcca ggcaaaagtc actttgcata
    10621 agaagagcga ttcttggctc atcaagggac cgtttggaga atacacagct gatcgtgtaa
    10681 ttttgacacc tggcccggca cttaagcctt tattacaaga attaggctat gctgcagata
    10741 tacgtccgca aaaagggcag ctcttagcat ttcagacatc ttataagatg agtcaagact
    10801 ggcctgttgc tatgcttgac ggagaagctg atttcattcc tttcatggat ggaaagatcc
    10861 ttttaggtgc tacccatgaa aacgatcaag gctgggattt ggctcctact agcgaagctt
    10921 ataagggatt gactaagagt acagaacagt tcttgactga tccagatcag ataaaaaact
    10981 ggccatacga gttccgtgta ggtacccgtg cttatacgtc agatttttcc ccatttttcg
    11041 gcgtactgcc agatgatcca agcttactgg ctgctagcgg tctaggctct tctggcttga
    11101 ccacaggtcc ttatatcggc tatctattag ccaacttctt aaatactggt cattggtcta
    11161 cggaagatta tcaaaaggac atctccacct atattacgaa aaatccatca tagaaaaaca
    11221 gctttttcaa gtaaggatcc cgagcgatat tgcgcatgga ttacttcttg aaaaagctgt
    11281 tattttagtt caatttctga atcaaattct ccaaactttc ttttgcttca tctggcaaga
    11341 gatcttttcc tatctgagct tcaatgaatc gtaaacgaat atcaagtcgt tgtttcacca
    11401 gatttttata ggacgtatcc tcaaaagaaa caggaaaatc tggtataggt aaatagtgga
    11461 tgtcttcaaa tggaccaaac gacttgaatt ctgccgtttg ttgcacgatt ctttcaacga
    11521 ttgacaatgt cgtttcaggt ttgatttttt tatcatcgaa atatccagta ttcaggacat
    11581 agcaggctgt ttgctgtttc aaaaacaact ctcgaaaagc ttgatagtct tccctaagag
    11641 gatagacacg gaaaggatta gcaaatggtt caatgactag ctcatctagc tgtctttttg
    11701 tagtgatatt ttccgcgtct gatcgttttg ttgctaatgt tgcaccaaaa gacgctgcta
    11761 aaactgggtc attgatcttg ataattggcg gaagcgaatc atctttcatg atccaaaaaa
    11821 cagcatcgat cttttcgcta agatgatcga ctcggtcggg tgtcgcataa cgtgatttga
    11881 ctgtccgccc atttccgttt cgtatatctc ctgttactag gactttttct cctttacttg
    11941 tacatgtcac gcccacattc tgacaagtaa ggaaagaaga aatggccgga tcatcagacg
    12001 gatagtcttg tgttttatca aagtaagaag gttccaaggc agtagcaaaa ccattttttc
    12061 gagaaataac gaaagcatcg tcatgcaaga tcgaaacaga gtgaccttcc gtatgttttg
    12121 ctaaggtaat cgttgatttt ccagaaccgg acaacccaaa tacagccatc gtataggtct
    12181 ggtcattact agcatattgt ttcatcccac cgtggcaggc aataaagcca ttgcgatttc
    12241 ccaatgccca agcaagcgtc aaagtcgctt tttttaattc tccaaagtaa cgtaatccta
    12301 gcacagccgc tgtattatgg agagcatcaa acaaaatgag tccatttgga aatgtttcat
    12361 gtgtccattc gggatcacaa aagatataaa gatctccctc atcaaaggaa acagactttt
    12421 gataagtctc taagatcttc gaattcgctg gctgaaaatt cagcaaataa gaatacaaat
    12481 tgttctcaaa tccttctgga atcagcaaat gactttgcac agaaaattct tcagataggc
    12541 caacgattac ttttgtatgg taaaactggc gcttgcgact ttgatacaca gcttctctta
    12601 aaattttctc gtatttgctt tgttccttgt ctaagtcctc aaagatccgc cgagcacttg
    12661 ctgttcttcc cacaatgctt ccatcattga atactagtgt cctaacatca gctggtaaat
    12721 ctagttcttc agcatgtgag attgttaaat ctgtgatgat cgttcctgga ctcccttttg
    12781 ctagttgata agctgtcttc ctgtcaaaaa cttcatgaac attattaccg taaaatgtcg
    12841 tttctacaat cgatcgaata ctggataaca gtggatttcc acttgtgatt tcgtttaatt
    12901 cgagactctc ttttgtactc atggctcctc ctcattccct aaacaaaaaa acgttttctt
    12961 tattatacat tattcgagtt tttatttgta ctgctgcgct gtgggaagca aacgagttat
    13021 ttttcacaca aaaaaaccga gaacttgaat aaacaagttt ctcggtttca tcattttcct
    13081 tttatctgct tagtaccagc cgtttgctaa ccagaatgtt ttagcagcat cccatgaacc
    13141 ataacgagat gttacatatt gttctgctac acgttcttga ttggcagctg aatagtcgcc
    13201 gtttaagtat gaagcatcta attgataacg gccgatgtaa cgtccgttag tagctgtgta
    13261 tgaaccgcct gattcttttt gtgcgatcca ttcttttgca gagcttgaag ttggcgctgt
    13321 tgcagcagct ggggcagcag gagctacttc aactgtttca acaggtgcaa cgtatgtatg
    13381 ttcttgcgtt gctgtttcag ttgtagatgc tgtattttct gttgttttgt tttctgttgt
    13441 tgctgaagaa tcatttgaag ttgggattgt taattgttca ccaacataaa tgcggttgat
    13501 gtttgcgatc gaattttttt cagcaattgc atcgatcatt gagttgtctc ctgcaaattt
    13561 ttgtgagatc ttagataatg aatcacctga ttttactgta taaacttcat cagcgtgagc
    13621 atttgtagtt cctacaaaca ttgcagtagc accagcagca aaagttgtac ctaaaacaat
    13681 tttcttaaat gaattcatat taaactcctt tttttctccg ttttttattc tttcgacgct
    13741 attaact
//
LOCUS       DACDKM010000075        13552 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20834_2016
            SAMN12640370-rid9891663.denovo.075, whole genome shotgun sequence.
ACCESSION   DACDKM010000075 DACDKM010000000
VERSION     DACDKM010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640370
            Sequence Read Archive: SRR10040891
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13552)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13552)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742835.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742835.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:04:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,012
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 29 of 75
            Pseudo Genes (incomplete)         :: 33 of 75
            Pseudo Genes (internal stop)      :: 19 of 75
            Pseudo Genes (multiple problems)  :: 5 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13552
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640370-rid9891663.denovo.075"
                     /isolate="E20834_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(370..930)
                     /locus_tag="IV334_001969"
     CDS             complement(370..930)
                     /locus_tag="IV334_001969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAP8944031.1"
                     /translation="MKKTDRRVKKTEKALAETLSTLLVNKKIQAITIRELTETADVHR
                     STFYTHYKDIYDLYDQLESNFFRDLNEILSYDPAHSYEELYTRLIDYVYANTFLYRLF
                     ILNHGDISFQKKVKQVIEENYLNIWLYEDKKTIITEEMRFFTTYHVQGCLSVLNRWVE
                     SNYKYSKKELLELLQKLNDHIEKIMP"
     gene            1072..1686
                     /locus_tag="IV334_001970"
     CDS             1072..1686
                     /locus_tag="IV334_001970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP8944032.1"
                     /translation="MKLTFKPLEKKDYKKAIYFAVEGMNFERYMENELLLNLYGRYFL
                     YMELNRATQILALYAEDELAGVLLAEIKGEKKKHRSFGQQIYVKIFDFLQNIFAKESK
                     GAYETANEQMLAAYLKKHSPDGEIIFLAANPDLKTKGIGTKLLTEFEKREKGKEIFLF
                     TDSGCTYQFYERRGFERLKEKISKIKILDKEIDLRCMLYRKIIQ"
     gene            1875..3755
                     /locus_tag="IV334_001971"
     CDS             1875..3755
                     /locus_tag="IV334_001971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HAP8944033.1"
                     /translation="MKLQKWIQEHRNHITALSAVLIIFGFIGKFTGFVLFYNIALAIA
                     SIIAVIPIMIHAYQAVKVKVISIDLLVTIAVLGAFMIGEYKESAIVTFLFLFGSYLEQ
                     KTLTKTRESIKALTEMAPSTASVVQEDGTTEEVEIDDVEEGDIVLVKTGGAIPVDGRV
                     IDGKGYADEAAVTGESRQVVKISGDEVFSGTILADGYVTIEATKVGDDTTFAKIIELV
                     EEAQDTKSHAEKFIDRFSQYYTPTVLLIALVVGLISRDFRLAITVLVLGCPGALVIGA
                     PVSNVAGIGNGAKRGILVKGGEAMDTFSKIDTFVFDKTGTLTKGTTAVSTIKNYTEDE
                     GDSLALVARLETLSDHPLGRAVIHYAQEKDIDFQQLAVKDNQTIKGQGITAEIDGHTV
                     CAGNTKLIAAHKLSLTPKQVQDLHQLQQTGSSVIIVAIDKKITQIIGVSDVIRPEVAE
                     QLAKLKQAGAKQLVMLTGDNQMTADYVADMLGIDEVHAELLPDEKVQFVKKYQEQGLR
                     VAFIGDGINDSPSLAAADIGIAMGSGTDVAIETSDVVLMQSNFESLVHAYRLAKKTVL
                     NTRENVVIAIGVVLFLLNGLFAGFIYMASGMFVHEASILIVIFNAMRLLYFGHESKEE
                     KS"
     gene            3834..4061
                     /locus_tag="IV334_001972"
     CDS             3834..4061
                     /locus_tag="IV334_001972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper resistance protein CopZ"
                     /protein_id="HAP8944034.1"
                     /translation="MKKIIMQLDTLACPSCMQKIEHAVSQQPGVSNTKVLFNASKIKA
                     NFDPDEVHPEQLAAVVEKLGYEVQSAKVKEA"
     gene            4066..4608
                     /locus_tag="IV334_001973"
     CDS             4066..4608
                     /locus_tag="IV334_001973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HAP8944035.1"
                     /translation="MNIVEEKFVQETKQADIDHHKPTAGAMVGHVLANFKLEEMKLIQ
                     AQLYVLGTSSQELSTLFSEYADQEARWFNKISHELVIENEIVPSTLEEVMRYGKIYEH
                     GQNKYLPASTMLENFAKDFDFQNVFITRAIKLAEKEEKYGLQQLLVELLSFNKEIIYQ
                     VQRLQGKTVRQDLDEEDDDD"
     gene            4835..5551
                     /locus_tag="IV334_001974"
     CDS             4835..5551
                     /locus_tag="IV334_001974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4767 domain-containing protein"
                     /protein_id="HAP8944036.1"
                     /translation="MKKRILVTIGLAFLLAGCTNGNESKKESTKETAKATVATTTTTS
                     TESKSTAASTETADTSTSSESSKTTETTQTSTSSSSVEASSSSSESSEQVEQTLWNAD
                     KSAQLEAFMTKWGQTMGQQYKSYTNQMSVDLYGLKVPQAILNGEWKMAIGGVPVSAEW
                     SESGTGQADYQITAVYSDAETEPYLKKHVYLFGFQQNQPKVLVTQQNQGNPDNYLYFN
                     ETANNELKNGFNQIVYGQAN"
     gene            complement(5611..7629)
                     /locus_tag="IV334_001975"
     CDS             complement(5611..7629)
                     /locus_tag="IV334_001975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HAP8944037.1"
                     /translation="MSLLVIFLFYIQKIRFIHRFIEYFAFDSIEQLTQRWKKRIHWLF
                     VHKSYFLVAAFFYCFTFVQIFVLEPKEGWKETIQVALKIFTQRQAPMILTIIIIMGFF
                     FLLLLISNRFWYALTATLIINLLLTISTVIKMEMREEPVFPSDLKMLTGLSELLSMVS
                     PVLLIVGGLILLLLLITSIIVQRRLQKQYSLKIHWKRRFISIAVLLGCFSGVFFINHK
                     NSPSFLLFNLFKVNKTFFNQQGAVKDNGPVIQFLNNIDIKIMEEPAGYSKEKINGIMK
                     KYNTKADEINQTRSDWLNNQTIVLNLSESFSDPARVPNLTVPTDPIPYIKSIITEGTG
                     GLMLSVGYGGGTANMEWEGLTGLSISNLSASLVTPYTQLVERQKISPNITDLFDEKIA
                     IHPFTAALYRRKQVFDKFGFDRFYYLDSPDKLTYTDKIEQSPRISDDSAYKETLKALQ
                     SNTETSQFIQLSTMQNHMPYDDYYSELNYTAEGTAVIPNRRKELQTYMQGLHYTDTAV
                     KKFIQEIDKIDKPITFVFYGDHLPAIYSGNDSKKYGLEQHQTDYFIYNNKYSREQAAT
                     LNKEVVAPNNFGAMALEHANIKVTPYYALLTEVSNHLPALTIDPTESLSNRFNGKQVF
                     VTEDSQIIQEKNLTEKQKEILEDYRLIQYDLVAGNQYSATWATQKIKD"
     gene            complement(7642..8640)
                     /locus_tag="IV334_001976"
                     /pseudo
     CDS             complement(7642..8640)
                     /locus_tag="IV334_001976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302657.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            8838..10079
                     /gene="sstT"
                     /locus_tag="IV334_001977"
     CDS             8838..10079
                     /gene="sstT"
                     /locus_tag="IV334_001977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine transporter SstT"
                     /protein_id="HAP8944038.1"
                     /translation="MLEKFLDMSLVQRIVVGILVGILFGIFFPTWTFISILGTLFVSC
                     LKAIAPILVFFLTMASIAKHKIGNKTFVKPILILYLVGTLLSALVAVIVSSIFTVPIT
                     LQETVSEKAPQSLESVLSTMLTNVTQNPVQSLIDANYLGVLFWAVLLGLAFRARSEST
                     KELVDQISIALSQVVQVIIAFAPIGILGLVYQSIATTGIAGLAEYLQLLLVLIGTMLF
                     VALVVYPFLTFLFIKENPYPLIFFCLKESALPAFFTRSSAANIPINMMLAERLKLTKE
                     SYSISIPLGATINMGGAAITISLMTLTAVHSLGIEAPFVLKVLLCILSALAACGASGV
                     AGGSLLLIPLACSLFGISNDVAMQIVGIGFIIGVIQDSMETALNSSSDLLFTAIGELG
                     ARKRSGEKIKLKDCLDGLSER"
     gene            10226..10861
                     /locus_tag="IV334_001978"
     CDS             10226..10861
                     /locus_tag="IV334_001978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF998 domain-containing protein"
                     /protein_id="HAP8944039.1"
                     /translation="MDFLRKFGFFFLLLGVLSDFLTPYVLGLYYPELDQMKSVISVFG
                     EVSSPVRKAFLIWSVVSGIFFILSLPALYAAFVSTSRVLATLVTAAIAFYGIGDCIFT
                     GLFSINTHESSWNVSTWVHNIGSGLGYSGFLLFPVLLFLIYRKNGQMDVSHLYLGLSV
                     ISLLFAAIYGLARIPSINSWPLLNKIGFCQRISFFFNYVPIARLSILHLKQ"
     gene            11052..11792
                     /gene="truA"
                     /locus_tag="IV334_001979"
     CDS             11052..11792
                     /gene="truA"
                     /locus_tag="IV334_001979"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HAP8944040.1"
                     /translation="MRNIKLTIEYDGKRYSGWQRLGDDDKTIQGKIEKVLHQMTNEEI
                     EIIGSGRTDAGTHARGQVANFKTNTEIGLAEMIDFMNRYLPRDIVIKRIEEMPERFHS
                     RYNAVGKKYSYYVWNNVIPSAFERNHSFYFPQELDMDKLNAACEKLVGTHDFIGFSAL
                     KKSKKSTVRTIDELSIERKGEMLHFTFVGDGFLYKMVRIIMGTLLEIGTGAMPIETID
                     EIFESKVRQHAGETVPAQGLFLDEVYYR"
     gene            complement(11937..13535)
                     /locus_tag="IV334_001980"
     CDS             complement(11937..13535)
                     /locus_tag="IV334_001980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhO family protein"
                     /protein_id="HAP8944041.1"
                     /translation="MLFLSFAYVIPLFMSKGIYHSVNQDTYFHLSRIIGLDNVWSSPV
                     NFNNFDHHGTMMNIFYPWLTLYPAFLFYKMMGNLVLSYNIYYFFITFLTMVVSYFSMK
                     QIKNNRYISLLFSIIYTFSAYRAIDIFHRASLGEAVALTFLPLILMGCYEIYIRDYQK
                     WYWLSIGMTLVVYTHLLSVAMVSVFIGGTLFLSFYFWDQKIARLLSLLKATVLTFFLS
                     AGFLIPFIQQSRAQELKVPLGKELSGMAPSDMLTHILNNNYNNYTIGLFLFLGLIGAF
                     IFIKKLTADDLFIFFLGVFVLFCSTNLFPWQLFNHTPVKSLQFVWRLNGFSSLFIAYT
                     MSIVIYYIFSTKNNSWKIMVFTFLALILHLSGVSNSIHANKDSLTLIAPEDAVAIAEN
                     YNHTDYANKESIYHPDMINNDQYLLGNQEINPTTHFMSNKLTIELDNSNNKNTVLTTP
                     IYRYKGQVASINGKLVQTKLSKFGTTELTIPPGINKVVITYQYTKLAIASRYLSIVTL
                     ILFLLYRFTFSKQKQPRREVQHSH"
BASE COUNT         4542 a         2139 c         2643 g         4228 t
ORIGIN      
        1 tgaagcgagc atggcgtaaa atgttagatg atgagctgat tccccttgaa aatgaattga
       61 ggtaaacgag aagaattcaa taaactggat gataaaaata taatatgact ataaattggt
      121 tacagcttca acctataagt tgtaataaat tagcctaaaa gtaatttatt aatgatgagg
      181 tgaatttgat tggtacaaca agatatatca cttagagagg gagaagcagc actacaaact
      241 acttttcaat aagtaggaat tatagaaatt ttactcaaaa aatcttcagt aaacaatttg
      301 gctaaataag attcagttgt tatacgagaa ggttacggtt cttcttaact aagcttagta
      361 tgtttttatt caaggcatga ttttctctat gtgatcattc agtttctgta agagttctaa
      421 taattctttt ttggagtact tgtaattgct ttctacccac cgattgagaa cggatagaca
      481 accttggacg tggtacgtag taaagaatct catttcttct gtgataatcg ttttcttgtc
      541 ttcatatagc catatgttca gatagttttc ttcaataact tgtttaactt tcttctgaaa
      601 cgaaatatca ccatggttga ggataaataa gcgatataaa aaagtatttg cgtaaacata
      661 gtcaatcaag cgagtgtata gttcctcata gctatgggct ggatcataag agagaatttc
      721 atttagatcc cgaaaaaagt tgctttctaa ttgatcatat agatcataga tatccttata
      781 atgtgtataa aaagtcgaac gatgtacatc tgccgtttct gttagttcgc gtatagtgat
      841 tgcttgaatc tttttattta ctaataatgt ggatagtgtt tcagccaaag ctttttctgt
      901 ttttttgacg cgtctatctg tctttttcat ttacctatct ccttatccga catatcaaag
      961 caaagctgtc gcttaatcga caaacttata gatgattgat tatagatagt agacactgtg
     1021 tctattataa tgtaagtata acacaatcat tgaagttgag gaggcgaaat gatgaaacta
     1081 acttttaaac ccttagaaaa gaaagattac aaaaaagcta tctattttgc tgttgaagga
     1141 atgaactttg agagatatat ggaaaatgaa cttctgctga atctttacgg aagatatttt
     1201 ttgtatatgg agttgaatcg agcaactcaa attcttgctt tgtatgcaga ggacgagttg
     1261 gcaggtgttt tgctggcgga gataaaagga gaaaaaaaga aacatcgttc ttttggacag
     1321 caaatatatg ttaagatatt cgatttttta caaaatatat ttgctaaaga aagtaaagga
     1381 gcttacgaga cagcaaatga acaaatgctt gcagcatatt taaagaagca ttcgccagat
     1441 ggagagatca tttttttagc tgcaaatcct gatttgaaaa caaaaggcat cggtaccaag
     1501 ttgctaacag aatttgaaaa aagagagaaa ggaaaagaaa tattcctttt tacagatagt
     1561 ggctgtacgt atcaattcta tgaacgacgt ggatttgaaa gactaaagga aaaaattagc
     1621 aagataaaaa tcttagataa agaaatagat ctccgatgta tgctgtacag gaaaatcatt
     1681 caatgaaaat ttcgatagaa acgacaatag ttggcaagat cacttttagt gagtcttctt
     1741 gctaactatt ttttgtagtg tattctacca aaagaaaacc gcctttttga ctaaacttga
     1801 tagtggtcaa ttttttatga tggataagta tctatactta gtctcgtaag aatgaaagga
     1861 aaagagggag aaagatgaaa ttacaaaagt ggatccaaga gcatcgaaat catatcaccg
     1921 ccttatcagc tgtgttgatt atttttggtt ttatcggaaa atttactggt tttgtcttgt
     1981 tctataacat tgctctagcc atagcttcta ttattgcggt catacctatt atgatccatg
     2041 cgtatcaggc agtgaaagtc aaagttatct caattgattt actcgttact attgctgtgc
     2101 ttggtgcctt tatgatcgga gaatacaagg agtcagctat cgttacattc ttgtttttgt
     2161 ttggtagcta tctggaacaa aaaacattga ccaaaacgcg ggagtcaatc aaggctttga
     2221 ccgagatggc cccatctact gctagtgttg tccaagaaga tggaacgact gaagaagtgg
     2281 aaatcgatga cgtggaagaa ggcgatatcg tcttagtaaa aactggtgga gctattcctg
     2341 tggacggacg agtcattgac gggaaagggt atgcggatga agccgcagtg actggtgaat
     2401 cacgacaagt cgtcaaaatc agtggagatg aagtcttctc tggaacgatt ttagcagatg
     2461 gctacgtgac gattgaagca actaaggtcg gagatgatac gacctttgcc aaaattatcg
     2521 aattagtaga agaagcacaa gataccaaat cccatgcgga aaaattcatt gatcgatttt
     2581 ctcagtatta tactccaacc gttttactta ttgcgttggt tgttggtctg attagtcgtg
     2641 attttcgttt agcaatcact gttttggttc ttggttgtcc tggcgcttta gtcattggtg
     2701 cgcctgtttc gaatgtagca ggaatcggta atggagcgaa acggggcatc ttagtcaaag
     2761 gcggtgaagc aatggatact ttttccaaaa tcgatacttt tgtctttgat aaaactggta
     2821 cattgaccaa aggaactact gctgtatcca ctatcaagaa ctatacagag gatgaagggg
     2881 acagcttagc tttagtggct cgattagaaa cgttatccga tcatcctctg gggcgcgccg
     2941 tgatccatta tgctcaggag aaagacattg attttcaaca attagcagtc aaagataatc
     3001 agacaatcaa gggacaagga atcacagctg agattgatgg tcatacagtt tgtgcgggca
     3061 acaccaaatt gattgccgct cacaaacttt ctctcacacc aaagcaagta caagatttac
     3121 atcaattaca acaaacaggc agctcggtca ttattgtagc aatcgacaaa aaaatcactc
     3181 aaatcatagg tgtgtcagat gtgattcgac cagaagtcgc tgaacaatta gctaagttga
     3241 aacaagcagg agcaaaacag ttagtgatgc tgacaggtga taatcagatg actgctgatt
     3301 atgtagctga tatgttaggc attgatgaag tccatgctga attattacca gacgaaaagg
     3361 ttcaatttgt gaaaaaatac caagaacaag gattgcgcgt tgcttttatt ggagatggaa
     3421 tcaatgattc tccctctttg gctgcggctg atatcgggat tgctatggga tcaggaacgg
     3481 atgttgctat cgaaacgagt gacgttgtct tgatgcaatc taattttgaa tcgttggttc
     3541 atgcctaccg cttggctaag aaaactgttt taaatacccg tgaaaatgta gtgattgcca
     3601 ttggtgtcgt cttattttta ctcaacggct tatttgccgg gtttatttac atggctagtg
     3661 ggatgttcgt tcacgaagct agtatcttga tcgttatctt taacgcgatg cgtttattgt
     3721 attttggaca cgaaagcaaa gaagaaaaat cgtaaaactt gataagaatc aattttttta
     3781 tggaagctgt aatctagaat aagactatca agaaaaagga aggaagaact acgatgaaaa
     3841 aaattattat gcaattagat acactagctt gtccaagctg tatgcaaaaa atcgaacatg
     3901 cggtttctca acagcctggg gtaagcaata cgaaagtttt gtttaatgcg agcaaaatca
     3961 aagcaaactt tgatccagat gaagtccacc ctgaacaatt agctgcagtt gtggaaaaat
     4021 taggttatga agtccaatca gcaaaagtaa aagaggcgta aatcaatgaa tatagtggaa
     4081 gaaaaatttg ttcaagaaac aaaacaagca gatatcgacc atcataaacc aactgctggc
     4141 gccatggtgg ggcatgtatt agcaaatttt aaattagaag aaatgaaatt gatccaagca
     4201 caattgtatg ttttgggtac atcttcgcaa gaattaagta cccttttttc agaatatgct
     4261 gatcaagaag cgagatggtt caataaaatc tctcatgagt tagtgattga aaatgaaatc
     4321 gttccgtcta ctttagaaga agtcatgcgt tatggaaaaa tttacgaaca tggtcaaaac
     4381 aaatatttac cagcctcaac aatgttggaa aactttgcaa aagattttga tttccaaaat
     4441 gtctttatca cacgggcaat caaattagct gaaaaagaag aaaaatatgg cttgcaacaa
     4501 ttgcttgttg aacttttaag tttcaataaa gaaatcatct accaagtaca aagactccaa
     4561 ggaaaaactg tccgacaaga tttggatgag gaagacgatg atgactaaaa gtctatgttt
     4621 cttcaaatac aaatataaag cgtgaggtaa attggaaaat cacgaagatt aaaataaaag
     4681 aagcacccgc tgatgtatta cagacaacat cagcgggtgc ttctcagttt ttagaagatt
     4741 tgaaagattg atgagaaaat acgaaaaatc aataaaatca cattgcttat ttgatagaat
     4801 aaagaagtgg gagtaatcga aaggagtttt ttaattgaaa aaaaggattt tagtaacaat
     4861 tggtctagct tttttgttag ctggttgtac caacgggaat gaatcgaaaa aagaatcaac
     4921 aaaagaaaca gcgaaagcaa cagttgcaac tactacaaca actagcacag agagcaagtc
     4981 aactgcagca agtacagaaa cagcagatac ctctacttcg tcagagtctt ccaaaacgac
     5041 tgaaacaact caaacatcta cttcaagttc ttctgtagag gcttcatctt cctcttcaga
     5101 aagcagcgaa caagttgaac aaacactgtg gaatgcagac aaatccgcac aattagaggc
     5161 atttatgacc aaatggggcc aaacaatggg acaacaatac aaaagttata cgaatcaaat
     5221 gagtgtggac ttatatggtc ttaaagtccc tcaagctatt ttgaacgggg agtggaagat
     5281 ggctattggt ggagtgcctg tttctgcaga atggtcagaa agtgggacag gtcaagcaga
     5341 ctatcaaatc acagccgtct attccgatgc agagacagaa ccatatctga aaaagcacgt
     5401 ctatctattc gggtttcagc aaaatcagcc aaaagtattg gtgacacaac aaaatcaggg
     5461 gaatcccgat aattatcttt acttcaacga gacagcgaat aatgaattga aaaatggctt
     5521 taatcaaatc gtttatggac aagcaaatta atgagataaa caaggggttg tgacatgtct
     5581 tttatcacaa ccccttgctc agtttatttg ttagtctttt attttttgtg tcgcccatgt
     5641 tgcactgtac tggttacctg caacaagatc atattgtatc agacgataat cttctagaat
     5701 ttctttttgt ttctcagtta aatttttttc ttggataatt tgactatcct ctgtaacaaa
     5761 gacctgtttt ccattaaaac gattagacaa gctttctgta ggatcaattg ttaatgcagg
     5821 tagatgatta gatacttctg tcaacaacgc atagtaagga gttactttga tattcgcatg
     5881 ctcaagagcc atagcaccaa agttattagg agcaacaact tctttattta acgtagctgc
     5941 ttgttcacga ctatacttgt tgttataaat gaaataatct gtctggtgct gttctaatcc
     6001 atattttttc gaatcattcc cactatagat tgcgggtaaa tggtcaccat aaaaaacaaa
     6061 ggtaatcggt ttatcgattt tgtcgatttc ttggatgaat ttttttactg ctgtatcggt
     6121 atagtgaagg ccttgcatat aagtttgtaa ttcttttctt ctattaggaa tgacagcagt
     6181 tccttcagca gtgtagttca attcactata gtaatcatca taaggcatat ggttttgcat
     6241 agtggataat tggatgaatt gtgacgtttc cgtattgctt tgtaaagctt tcaaggtttc
     6301 tttgtatgct gaatcatccg atatgcgtgg gctttgttct attttatccg tataagttaa
     6361 tttatccgga ctgtctaaat aatagaacct gtcaaaacca aatttatcaa acacttgttt
     6421 tctgcgataa agagcagctg taaaaggatg gatggctatt ttttcatcga atagatcggt
     6481 aatatttgga cttattttct gacgctccac tagctgggta taaggtgtaa ctaacgaagc
     6541 agacaggtta ctgatactca agcctgtcag tccttcccat tccatgttag ctgttccgcc
     6601 accataaccg acggataaca tcaatcctcc tgttccttct gttataatgc ttttgatgta
     6661 aggaataggg tctgtaggta ctgtcagatt agggacacgt gcaggatcac tgaagctttc
     6721 acttaaattg agaacgatcg tctgattgtt taaccagtca cttcttgttt ggttgatttc
     6781 atctgccttt gtgttgtact ttttcattat tccattaatt ttttctttcg aatagccagc
     6841 aggttcttcc atgattttaa tgtcgatatt gtttaggaat tgaatgactg gtccgttatc
     6901 ttttacggcg ccttgctggt taaagaaagt cttgtttact ttaaataaat taaacagcaa
     6961 gaaagaaggc gaatttttgt gattaatgaa aaagacaccc gaaaaacatc ctaataatac
     7021 agcgatactg ataaatcgac gtttccaatg aatcttcaag gaatactgtt tttgtagtct
     7081 acgttgtaca ataatactag taatcaataa taggagaagg atcaatccgc ccacgattaa
     7141 taagactggg ctaaccatcg ataatagttc tgacaatccg gtcagcattt ttaaatctga
     7201 aggaaagaca ggctcttccc gcatttccat tttgattact gtcgaaatag tgagcagtaa
     7261 gttaatgatc aaggtagcag tcaaggcgta ccaaaaacga ttggaaatca acaatagtaa
     7321 gaagaaaaat cccataataa tgatgattgt caggatcatg ggtgcttgtc gctgagtaaa
     7381 tatttttaag gcaacctgga tagtttcttt ccaaccttct tttggttcta aaacgaaaat
     7441 ctgcacgaag gtaaagcagt aaaaaaatgc tgcaactaaa aaataacttt tatgaacaaa
     7501 aagccaatga attctttttt tccaacgctg agttaattgt tcaatcgaat caaaagcgaa
     7561 gtattcgatg aaacgatgaa tgaaacggat tttttgtata taaaataaaa agataactag
     7621 gagggacact gctaataaat atcaaataaa aaactgcccg atatttatca gccatgcctt
     7681 ccctgagttt ggaaattcac gtttcataaa tgtagatacg cgttaagaaa ctaaagggaa
     7741 attacatata acttgagtga caatcaaaaa tacagcaaga aaatttcctc ttccttttat
     7801 acctaattta tcagatagca tttctaaaaa taaaaacgaa gagaaagtat aatacattgc
     7861 gaagagagaa tatggtttag aaaacctcag cgctgtatct gtatttcctc tgatgatgag
     7921 tgaaatttgt gtcatcaaaa taatcaaacc gaataaaaca atcaagctga taaatacttg
     7981 aggaagctta tagcggacct tttctttcca gttccattct ttcaaaccat aacccattaa
     8041 aaataaataa aaaatccata aaatactctg tccattctgt aatgcaaacg ggtctttatt
     8101 aaatagagta gagcagccaa ctaatccaat tgttaaaaat aatagtagat atttaattgt
     8161 tgttttcgat aatttattta aaaatccaac tgtataagga atactacaaa aaagcaaagt
     8221 acaagaaaca gcataagtaa agtaattctg actgataggt gttaggataa gccaataatc
     8281 acgaatattc aagctgccag cttgtacgta aaaaaagata ttgcaaacta gtgtggctat
     8341 taggaatata aaggcaaaac gacttaaata atgtaaaact tttaaacggg cgattttttg
     8401 atattggtaa cccatgtaaa gtgggatcaa atttgcacca ataacaacgg aagtttgtaa
     8461 aaatgtatag atgtagaatc gaccagggtt tgttgcatat gtaggaatag tgactgctga
     8521 aatcaatata cgctgtaaaa ccatcattat acaagcgaca aagattgtca ggaagccata
     8581 actctccttc tttatttttt tcaagaataa acacctcaca aaaaaattgg acaaaaacat
     8641 aagaaaacgt ttcctgataa gttgcccatt aataacatcc taacttagaa tatctcaaaa
     8701 aacaacgtgt agacagaaaa agtacaaaat tagcaagaaa tggttgtttg tattgttaga
     8761 atttttagaa aattatacct ttagtttctt tgcataaaat gatacaatga ttgttaaaat
     8821 tgtgaaaggg tggtcatttg ttagagaagt ttttggatat gtcgttagtt caacgtattg
     8881 ttgttggtat tttagtcgga atattgtttg gtatcttttt ccccacatgg acatttattt
     8941 caatattggg aacgttgttt gtcagttgct tgaaggcaat cgcacctatt ctggtttttt
     9001 tcttgacgat ggcgtcaatt gccaagcata aaattgggaa taagacattc gtgaagccta
     9061 ttttgatctt atatttagtg ggaacattgc tttctgcact tgtagcagtt attgtaagtt
     9121 caatttttac tgttcctatt actttacaag aaacagtatc tgaaaaagca ccgcaaagtt
     9181 tggaaagtgt tttatctact atgttgacga atgtcacaca aaatcctgtt caatctctta
     9241 ttgatgcgaa ttaccttggc gtcttattct gggctgtatt gttgggacta gcttttcgag
     9301 cacgttcaga atcgacaaaa gagttggtgg atcagatatc tatagcgttg tcacaagtcg
     9361 tacaagtgat tattgcattt gctccgatag ggattttagg attggtttat caatcaattg
     9421 ctacgactgg aatcgctgga ttggcagagt atctgcagct tttacttgtt ttgatcggta
     9481 cgatgctgtt tgtcgcatta gttgtgtacc cattcctcac gtttttgttt attaaagaaa
     9541 atccctatcc attgattttc ttttgtttga aagaaagcgc acttcccgct ttctttaccc
     9601 gaagttctgc cgcaaatatc ccgataaaca tgatgttggc tgaaagattg aaattgacaa
     9661 aagagtctta ttccatatcg atcccgttag gtgctacgat caatatgggc ggtgccgcga
     9721 ttaccatttc cttgatgacg ttgactgctg tacattcttt agggattgaa gcaccttttg
     9781 ttttgaaagt ccttttatgt attttgtctg ctttagctgc ttgtggagct tcaggtgttg
     9841 cgggcggttc actgttattg attccattag catgtagttt gtttggtatc tctaatgatg
     9901 tcgcgatgca aatcgtcggt attggattta ttatcggtgt tatccaggat tcaatggaaa
     9961 ctgccttaaa ttcgtcaagt gatctgttgt ttactgcaat aggagagcta ggcgcgcgta
    10021 aaagaagtgg ggaaaaaatc aaattgaaag attgtttaga tggattaagt gaacgttgaa
    10081 cgttagatat ttagtatttt taaaatgatt cgagttttta atgagttata tagatgttcg
    10141 tgtaaataga ggtttctgtt aaatcaagat aaagtcgttt ataataacaa ttaaaagcat
    10201 aggtaattgt tattggaggc gtatcatgga ttttttacgt aaatttggtt ttttcttttt
    10261 attattaggc gtgctgagtg attttttgac tccgtacgta ttaggattgt attatcctga
    10321 attagatcaa atgaagagcg tgatcagtgt atttggagag gtttccagtc ctgtaagaaa
    10381 agcgtttctt atttggtctg tggtttcagg gatctttttc atcctgtcat tgcctgcatt
    10441 gtatgctgca tttgttagta catctcgtgt gttagctacc ctagttacag cagccatcgc
    10501 gttctatggt ataggtgatt gtatctttac aggtttgttc agtatcaata cccatgaatc
    10561 ttcgtggaat gtctcgacat gggtccataa tatcggatca ggtttaggtt acagtggttt
    10621 tttattgttt ccagttcttc ttttcttgat ctatcgaaaa aatgggcaaa tggacgtaag
    10681 ccacttatac cttggactgt ctgtgatcag tttgcttttt gcggcgattt atggactggc
    10741 aagaattcct tctataaatt cttggccatt actgaataaa ataggttttt gccaaagaat
    10801 cagttttttc tttaattatg tgcccattgc caggttgagt atcttgcatc tcaaacagta
    10861 aaacttgagc cgtcaaattg aacagtcagc ggatacttca tccggtggct gttttttgtt
    10921 tttctgaaat atatatttcc acagttttct tgtatagctt ctgtcttaga gaagcaagga
    10981 gctatatttt gtgctacaat cttaaaaagg tagagagaag accaaatgga agacagagga
    11041 gccactaaaa tatgagaaac atcaagttga caatcgaata tgacggaaaa agatattcag
    11101 gatggcaaag acttggagat gacgataaaa ccattcaagg aaaaatcgaa aaagtattgc
    11161 atcagatgac aaatgaagag atagaaatta ttggatcagg aagaacagat gctggcaccc
    11221 atgcgcgagg acaagtagca aattttaaaa caaacacaga aattggactg gcagaaatga
    11281 tcgactttat gaatcgttac cttccgcggg atattgtcat caaaagaatc gaagaaatgc
    11341 ctgagcgctt ccattcaaga tataatgcgg tgggcaaaaa atacagctat tatgtttgga
    11401 acaacgtgat tccttcagca tttgagcgaa accacagctt ttatttccct caagagttgg
    11461 atatggacaa gctgaatgca gcttgcgaaa aattagtggg tacacatgat ttcattggtt
    11521 tttctgcatt gaaaaaatca aaaaaatcca cagtgcgtac gattgatgaa ttatctatcg
    11581 aacgaaaggg agaaatgctt cattttactt ttgtcggaga cggattctta tataaaatgg
    11641 tgcggattat catggggaca ttactagaaa tcggcacagg tgccatgccg atagaaacaa
    11701 tcgatgaaat atttgaaagc aaagtgcggc aacatgcagg agaaaccgtc cctgcacaag
    11761 gtctgttttt ggatgaagtt tattatcgat aaaaatactt tgataatgga tgaaacgcat
    11821 gtttcacagt ataaatatcg atgttaaata atgtatgaag caaattaaag aaatataaaa
    11881 aaagactttt tagcctgttt atgtgtacag ctaaaaagtc tttcttaaat aaaaatctag
    11941 tgagaatgtt gtacttctct tctcggttgt ttttgtttag aaaaagtaaa tcgataaagt
    12001 aaaaataaaa tcaaagtaac gatagataaa tatcttgaag caattgctag ttttgtgtac
    12061 tgataagtaa taacgacttt atttatacct ggaggaatag ttaattcagt tgtgccaaac
    12121 ttagataatt ttgtttgtac tagtttgcca ttaatagaag ccacttgccc tttataacga
    12181 taaataggtg tcgtcaagac agtattttta ttattcgaat tatctaattc aattgtaagt
    12241 ttgttactca taaaatgagt tgttggattt atttcctggt tacctagtaa atattgatca
    12301 ttattaatca tatcaggatg atagattgac tctttgtttg cataatcagt atgattgtaa
    12361 ttttcagcta tagcaacagc gtcttcaggt gctataagtg ttaatgaatc tttgttagca
    12421 tggatagaat tgcttactcc tgagagatgc aatattagtg caagaaacgt aaagaccatt
    12481 attttccatg aattattttt agtagaaaaa atataataga tcactatact catcgtatag
    12541 gctataaata aagaagaaaa accatttaac cgccagacaa attgtagact cttaactggt
    12601 gtatggttaa aaagttgcca aggaaataaa ttggttgaac aaaaaagaac aaatacacca
    12661 agaaagaaaa taaataaatc atcagcagtt aattttttta taaaaataaa agcaccaatc
    12721 aatccgagaa ataagaataa tccaatagta tagttgttat agttattatt taaaatatgt
    12781 gtcagcatat cactaggagc cataccactt aattcttttc ccagtgggac tttcaattct
    12841 tgtgctctag attgttgaat aaaaggaatt aagaatcccg cacttaaaaa aaaggtgagg
    12901 acggtagctt ttaaaagact taagagtcta gcgatttttt ggtcccaaaa ataaaaagaa
    12961 agaaacaacg ttcctccaat aaatacagaa accatagcga cagaaagtaa atgagtatac
    13021 actactagcg tcatgccaat agacaaccag taccattttt ggtaatctct aatataaatt
    13081 tcgtaacacc ccattagtat caaaggcaaa aatgttaaag cgacagcttc tcccaatgaa
    13141 gcacgatgga atatgtcaat ggcacgatag gcggaaaatg tataaataat cgaaaataac
    13201 agcgaaatat aacgattgtt ttttatttgt ttcatagaaa aataagaaac gaccattgtt
    13261 aaaaaagtga taaaaaaata gtagatatta taacttaaaa caagatttcc catcatttta
    13321 taaaataaaa aagctggata aagagtaagc catggataaa aaatattcat cattgttcca
    13381 tgatgatcga agttattgaa gtttactgga gaactccata cattatctag tcctatgatt
    13441 cgagataaat gaaagtaagt atcctgattg acactatgat agattccctt agacatgaat
    13501 agagggatga cgtaagcaaa acttagaaat aacataaaaa tgatatctat ta
//
LOCUS       DACDKM010000076        13398 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20834_2016
            SAMN12640370-rid9891663.denovo.076, whole genome shotgun sequence.
ACCESSION   DACDKM010000076 DACDKM010000000
VERSION     DACDKM010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640370
            Sequence Read Archive: SRR10040891
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13398)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13398)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742835.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742835.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:04:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,012
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 29 of 75
            Pseudo Genes (incomplete)         :: 33 of 75
            Pseudo Genes (internal stop)      :: 19 of 75
            Pseudo Genes (multiple problems)  :: 5 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13398
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640370-rid9891663.denovo.076"
                     /isolate="E20834_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(14..706)
                     /locus_tag="IV334_001981"
     CDS             complement(14..706)
                     /locus_tag="IV334_001981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HAP8944042.1"
                     /translation="MQIELQHTHDKETFEKNYKVYFELARDSAMKYGIPLTLDTPYNQ
                     PGIKSHLWVTQNIWGDHTDPYGYLSEMGVSKEKLAYDLAHGFTDDNPTTSEDKPVIDP
                     TRAGAANSTLTDGTNYAHIDQFGEIENANLHVAGWHIANYKYEYIFIMDYNTGKELAR
                     VRADGIYRPDVNQAYNTLGNVGYHVSFNMRDFPNKKVYVMMRATNDPEGNTKGGAQDF
                     HDKRWYLNIPQR"
     gene            791..967
                     /locus_tag="IV334_001982"
                     /pseudo
     CDS             791..967
                     /locus_tag="IV334_001982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348541.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase"
     gene            complement(1143..1712)
                     /locus_tag="IV334_001983"
     CDS             complement(1143..1712)
                     /locus_tag="IV334_001983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HAP8944043.1"
                     /translation="MELKKLEVPTSSITEVKRSPMDVFAQARDAGTGVYIFNREKVAG
                     VMLTQEQYETLLQELDVLRQTVKVERKEPKEINEEPINKTASVVSNAEGMEELAQTLQ
                     HSLITGVTITAKNLDERMVALGFITKKTGFGGVVDMLSELMETGKINYQLRRKPQNRT
                     IIAEIIGEQNSQSQLMDKIIIKKIFLHEL"
     gene            complement(1786..2109)
                     /locus_tag="IV334_001984"
     CDS             complement(1786..2109)
                     /locus_tag="IV334_001984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8944044.1"
                     /translation="MLLLKTLQERGYLDVHPPEVKIVFFFRYEHQEARRLAGVLNKTL
                     RHRKRISIHVCTRNKPPRYADLVIIDHFFPLDHNEDQKTYLYHPSFGIERLLTDINYW
                     LGKNR"
     gene            complement(2263..3075)
                     /locus_tag="IV334_001985"
     CDS             complement(2263..3075)
                     /locus_tag="IV334_001985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008274430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8944045.1"
                     /translation="MRWQKLLSKNAQNLFSLINLMKDLIWRKADLAEELKIDVKTVDR
                     HLKRLAESDCPIQLILHKKTVQLVYDQGYSEPYLLFVFLIQSPPFCLLKNLLLNEPYE
                     QKYDRSTQERLRKWFGEYHLSFSYKKAQIFGSERKVRYLLFCFLKDFPHFFADDGEQS
                     FSEMFIQLLKQRKDPAEIEILGDSRTLFFELFPELLFREEQILPIKEKNYLAILRLSC
                     NQITEKRWKRIEETQDYRKLTIVTEKLDSLFWLNSEFESESKSKLTKVLYQE"
     gene            3429..4277
                     /locus_tag="IV334_001986"
     CDS             3429..4277
                     /locus_tag="IV334_001986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008274428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HAP8944046.1"
                     /translation="MIVALLAGGHVLFEDIPGVGKTLLVKALAKAVQGTFSRVQCTPD
                     LLPSDILGFSVYNSQSKEFEFRPGPIFTTILLADEINRTTPRTQSALLEAMAENHATI
                     DNNTYPLDNHFFVLATQNPIEYEGTYPLPEAQLDRFLFRLQIGYPSFDDELLLLLDKF
                     EKVLDNLNEVLNSYEIEEIKTNVANVFVSPEVASYALQLVSATRSHAAIQLGISPRGS
                     LSFIQAAKAYALIHGRNYVTPKDLQDLVPYVFSHRLIFYDRSLKEDEKKQIIHTLVAQ
                     IPIPVR"
     gene            4422..5363
                     /locus_tag="IV334_001987"
     CDS             4422..5363
                     /locus_tag="IV334_001987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HAP8944047.1"
                     /translation="MELDFIKQLSKETFGYLYIAATRLSQKKYAGYFFHSKKENFFFT
                     SPSFTNRLPSNLCRKKQEILILTNKPQEVQLLWQPKERGCHETLTVIFTAYDSLGLFR
                     KSAKREISFPVTVLPAFCKIQAEKLQRVLEKKQQLNAYQRGLDFREHRAYRPGDSFNR
                     IDWKLSAHTQEWLYREYDYQEEECSPLFCFWGLPSPKFEPLLDLYFSLWHLRKEKQPE
                     LLILGNRAFHGKDPGHTYFASLEAGDEKAFFAHLKKYAKKQIVLFVPENSPEVSEAIE
                     TLSKDRTVYLVYFVEKQLMVQEKDKICSLPGGEWIDD"
     gene            5353..7524
                     /locus_tag="IV334_001988"
                     /pseudo
     CDS             5353..7524
                     /locus_tag="IV334_001988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343611.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase domain-containing protein"
     gene            complement(7636..9807)
                     /gene="copB"
                     /locus_tag="IV334_001989"
     CDS             complement(7636..9807)
                     /gene="copB"
                     /locus_tag="IV334_001989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper/silver-translocating P-type ATPase CopB"
                     /protein_id="HAP8944048.1"
                     /translation="MNGNDMEKKHEHKKEVVNKESTESHEKQMEMKHDHSQMDHSMHM
                     GHNHGDIANSKQMDHSMHMDHEHGGMDHSMHMGNFKQKFWLSLILAIPIILFSPMMGM
                     EFPFQVTFPGSDWLVLILATILFIYGGQPFLSGAKMELKQKSPAMMTLIAMGITVAYI
                     YSVYSFIANLLNPHTHVMDFFWELATLIVIMLLGHWIEMNAVSNASNALQKLAELLPE
                     SVKRLTKDGKEETVSLKEVNEGDRLIVRSGDKMPTDGVILKGETIVDESAVTGESRGI
                     KKQANDKVIGGSINGDGTIEIEVTGTGENGYLAKVMEMVRKAQGEKSKLESLSDKVAK
                     WLFYVALIVGILAFIAWLFLTDLPNALERMVTVFIIACPHALGLAIPLVVARSTSIAA
                     KNGLLLKNRNALEQANDLDVIMLDKTGTLTEGKFTVTGVEVLDDAFNKNEILQYLGAL
                     EANANHPLAVGIMNYLKEHEIKPYQAENLKNLSGVGLEATVKKQQVKIVNEKEVERLG
                     LNVEQALLKPYQEQGNTISFLILENHLAAIVALGDVVKTEAKEFIRTLKERKITPVML
                     TGDNKNAAQAVADYLGIEEYYGGLLPDDKEAVVQKYLDQGKKVIMVGDGINDAPSLAR
                     ASIGMAIGAGTDIAIDSADVVLTNSDPKDILHFLDLAKQTRKKMIQNLWWGAGYNILA
                     IPLAAGILAPIGIILSPAVGAVLMSLSTVVVALNALTLKIR"
     gene            complement(9827..12013)
                     /locus_tag="IV334_001990"
     CDS             complement(9827..12013)
                     /locus_tag="IV334_001990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper-translocating P-type ATPase"
                     /protein_id="HAP8944049.1"
                     /translation="MATAETKMDTFVITGMTCANCSARIEKELNGQPGVVNATVNLAT
                     EKASVKYEGTTTEKLIQSVENIGYGAILYDEAHKQKIAEEKQAYLKKMLFDLILSTVL
                     TLPLMLSMIAMMLGSHAAIVHFFHFPIVQLVLSAPVQFYVGARFYKGAYHAIKTKAPN
                     MDVLVAIGTSAAFALSIYNGFFRGHPQDLYFESSSMIITLILLGKYLEHTAKTKTGNA
                     IKQLMSLQTKTAQVIRNGKEETLAIEEVVVGDQLVIRPGEQIPADGRIISGSSAIDES
                     MLTGENLPVEKNPDDTLFGGTINTNGLLHMEVTQVGKQTVLAQIIQMVEDAQGSKAPI
                     QKIADRISGIFVPIVLVIAFITLIATGLITGDWQLALIHSVSVLVIACPCALGLATPT
                     AIMVGTGVGARNGILIKGGEALEAAAHLDSIVLDKTGTITEGKPKVTDLVGSKEVLSI
                     FYTLEQASEHPLGKAIVEYGKLQEAATYDMIDFTAHPGAGISGTINGVRYFAGTRKRL
                     IELNLSFDEYQEHALELEQQGKTVMFLADEKQVIGLIAVADQIKLEVKQAIKQLQNKG
                     LDVFMLTGDNKLAAETIGKQVGIDPKHIFAEVLPEDKAAYVEKLQKDGKKVGMAGDGI
                     NDAPALALADVGMAMGSGTDIAMETADVTLMNSSLASIAQTIELSRLTLRKIKQNLFW
                     AFVYNTIGIPFAALGFLNPIIAGGAMAFSSVSVLLNSLCLNRHMKK"
     gene            complement(12013..12222)
                     /gene="copZ"
                     /locus_tag="IV334_001991"
     CDS             complement(12013..12222)
                     /gene="copZ"
                     /locus_tag="IV334_001991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper chaperone CopZ"
                     /protein_id="HAP8944050.1"
                     /translation="MKQQFSIEGMSCNHCAARVEETVSALDGVQKVKVNLKKANGTVK
                     FDETKVQSEKICQAINGLGYKAEVI"
     gene            complement(12235..12675)
                     /locus_tag="IV334_001992"
     CDS             complement(12235..12675)
                     /locus_tag="IV334_001992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopY/TcrY family copper transport repressor"
                     /protein_id="HAP8944051.1"
                     /translation="MEKVKTPVKISDSEWEIMRVIWTLGQTTAQEITQILADSMDWKP
                     ATIKTLLGRLVKKEVIWTEQEGKKFIYYPAVSEMETVRSATENLFSHICAKRIGETIA
                     DLVEEATLTQEDINEILKQLEQKQPVDTIECNCIPGQCECKQHQ"
     gene            complement(12749..13288)
                     /locus_tag="IV334_001993"
     CDS             complement(12749..13288)
                     /locus_tag="IV334_001993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAP8944052.1"
                     /translation="MKIIVIGQSGSGKSTLARKIKEITDFPLLPLDLLWHTTDYSIQA
                     KKWFLQEQQLFMVSNPSWIVEGNYTSTLEERIKEADKIIWLKVPRYLAIYRVVWRSLK
                     RKINKKSRPDMPESFSEKLNREYLEFLSFIWHFEENNEPKIQQLIQVANARNKLIILK
                     TRREKQEFLTKLKIESKNN"
BASE COUNT         3765 a         2807 c         2380 g         4446 t
ORIGIN      
        1 agggggggat tttttatcgt tgtggaatat ttaaatacca acgcttgtca tgaaaatctt
       61 gtgctccacc cttagtattt ccttctggat cgtttgtcgc acgcatcatg acgtatactt
      121 tcttattagg gaaatcacgc atattgaaag atacatgata gccaacattt cctaaagtat
      181 tataagcttg atttacgtct ggtctataaa tcccatcagc tcttactcta gctagttctt
      241 ttccagtatt gtagtccata atgaaaatat actcgtattt ataattagca atgtgccatc
      301 cagctacatg caagtttgcg ttttcgattt ctccgaactg atcaatgtgg gcgtaatttg
      361 ttccatctgt cagcgtagaa tttgcagcac ctgctcgagt tggatcaatt actggtttat
      421 cctccgaagt agttggatta tcatccgtaa aaccatgagc caaatcatag gctagttttt
      481 ctttacttac acccatttca gaaagataac cgtaaggatc tgtatgatcg ccccaaatat
      541 tttgtgttac ccataaatgc gatttgattc ctggttggtt ataaggagtg tctaatgtta
      601 atggaatacc atatttcatt gctgaatctc ttgccaattc aaagtatact ttatagttct
      661 tttcaaaagt ttctttatca tgtgtatgtt gtaactcgat ttgcacagga cttttggctc
      721 acgaggttgt cctactctac atcttgctga tagatatgct gaaaggaaaa caccaacatt
      781 atgttttgct ttttcagcta gaacgagaca agcaggaata cctttagaag atatcaaagg
      841 ttttttaggt cataaagatg tttccactac acaagtctat gcccatattt ctcccgaagt
      901 gaaaaaaaga tcaatgaatc aactagaaaa ctatatagaa gagcaaataa aaaagcactc
      961 aaattgagtg cttttttaag ctcactatca ctaaaactat cactaatttt atttataagc
     1021 gaacatgttt ctatcactga ttttttaaat ctagcgaaaa acccttatct taaaggcgtt
     1081 cgttcaattc tttggcaagg tcttcaaagc ctggcttgcc tagaagtgcg aacatggttt
     1141 tcttatagct catgtagaaa aattttcttg atgatgattt tatccatcaa ttgagattga
     1201 ctgttttgct ctcctatgat ttcagctatg attgtccgat tttgcggttt tcttcttaac
     1261 tggtagttga ttttcccagt ttccatcaac tcgctaagca tatccacgac tccgccaaat
     1321 ccggtctttt tcgtaataaa acctagtgct accattcgtt catctagatt ttttgctgta
     1381 atcgtcacac cagtaatgag cgagtgctgt aatgtttggg ccagctcttc catcccttca
     1441 gcattggata cgacggaagc tgttttgtta ataggttctt cgtttatttc ttttggttct
     1501 ttcctctcca ctttcactgt ttgacgaagg acatctagtt cttgcaagag cgtttcgtat
     1561 tgttcttggg tcagcatcac gccagctact ttttcacgat taaatatata tacgccagtt
     1621 cccgcgtctc gcgcttgagc aaatacatcc atcggcgatc gtttcacttc tgtgatcgaa
     1681 gatgtgggaa cttctaattt ctttaattcc aatggatttc ctcctttact aatcgctttt
     1741 tattatagca cagactgact tgttttgatc ttttttccaa aggttttatc ggtttttgcc
     1801 taaccagtaa ttgatatcgg taagaagtct ttcgatacca aatgaaggat ggtacagata
     1861 cgttttttga tcttcgttat ggtctagtgg aaaaaaatga tcgatgatca ctagatctgc
     1921 ataccgaggc ggcttatttc gagtacatac atgtatagag atccgttttc gatgtcttag
     1981 cgttttgttc agtaccccag caagtcttct tgcttcttgg tgttcatagc gaaaaaaaaa
     2041 gacgattttc acttcaggag ggtggacatc taaataccct cgttcctgga gtgttttcag
     2101 taaaagcatc ccccactcta ctgcggggtg tgcaagtgct tgttggatcg ctagccgaaa
     2161 ttgatatagt ttatctttgg aattgggaaa tttttctaat gtttcctgat aataacgaac
     2221 ttcttctatc agccggtcat ggataccaat tacgtaacga agtcattctt ggtaaagtac
     2281 ttttgtcagc tttgatttgc tttcgctctc aaattcgcta ttcagccaaa acagcgaatc
     2341 aagcttttca gtcacaatcg tcaatttccg atagtcttgt gtctcttcta tccgtttcca
     2401 ccgcttttca gtaatctggt tacagctcaa gcgcaaaatt gctagataat ttttttcttt
     2461 tattggcaaa atctgctcct ctctaaatag caattcagga aacaattcaa agaatagtgt
     2521 tcgactgtct cctaaaatct cgatttctgc cggatccttt ctttgcttca ataattggat
     2581 gaacatctca gaaaaggact gctctccatc atctgcaaaa aaatgcggaa aatctttcaa
     2641 aaagcaaaac agcagatatc ttacttttct ttctgaacca aagatctgtg cttttttata
     2701 agaaaaagaa aggtggtact cgccaaacca ctttctcaat cgctcttgcg tagaacgatc
     2761 gtacttctgc tcgtaaggtt cattcaatag cagatttttc agtaaacaaa acgggggtga
     2821 ttggatcaga aaaacaaaca agagataagg ttcgctatat ccttgatcat aaactaactg
     2881 aacggttttc ttatgaagaa tcagctgaat gggacagtcg ctttccgcca aacgtttcag
     2941 atgccgatcc actgttttaa catcgatttt cagttcttca gctaaatcag cttttcgcca
     3001 gatcaaatct ttcattagat tgattagtga aaataaattc tgtgcatttt ttgataatag
     3061 tttttgccat ctcatcgatc atttctcacc tgatttctaa acatattgtt tgatcaaaat
     3121 gggttcactc ctaaaaagat aaaaaactcc ctttctattt atttaaatac gcaaatcttc
     3181 ctcattattt tttattttac tcaatttaat cgtattctca tttcaaaacg aatgtacttt
     3241 aaaaaaacat aataagatca ctttgcttgt atgatgtatt cccttccatt acaatgaaat
     3301 aaatagcgat tttatgagaa ggtgatgtga aaatggaaga aaaaatagaa gaatccaaac
     3361 aaaaaatcga tcagttgata taagaattag aaaaagcggt catcagcaaa cgttcaacta
     3421 tccaattgat gatcgtggcc ctattagcag gtggtcatgt cttattcgaa gatattcccg
     3481 gtgtaggtaa aacgttactg gtcaaagcac tagcaaaagc tgtacaggga accttcagca
     3541 gagtccaatg tactcctgat ctgctgccta gtgatattct tggtttttcc gtatacaatt
     3601 cacaatcaaa agagtttgaa tttcgtccgg gaccgatctt taccacgatc ttgttagcag
     3661 atgagatcaa tcgtacgact cctcgtactc agtcagcact tttagaagca atggccgaga
     3721 atcatgcaac gatcgacaac aatacatacc ctctagacaa tcattttttt gtcttagcta
     3781 cgcaaaatcc aatcgagtac gaaggaacat atcctttgcc ggaagcacag ctcgatcgtt
     3841 ttttattccg actacaaatc ggttatcctt cttttgatga cgaacttttg ctacttttgg
     3901 ataaattcga aaaagtgttg gataacttaa atgaagtatt gaacagttat gaaatagaag
     3961 aaataaaaac aaatgtggcg aatgtctttg tcagtcccga agtggcatct tatgccttgc
     4021 agttagtgtc agcaacaaga agtcatgctg ccatccagct tggcatcagt ccacgaggaa
     4081 gcctttcctt tattcaggca gcaaaagctt atgctttaat ccatgggaga aattacgtca
     4141 cgcccaaaga tttgcaggat ttagttcctt acgtgttttc ccatcgcctg attttttacg
     4201 atcgttcatt gaaggaagac gaaaagaaac aaatcattca tactcttgtt gcacaaattc
     4261 ctattcctgt gaggtagatt gaggtagaca atggaaaaaa tcaaagatta tttcattaat
     4321 tttgtgttat ttctggtata tttcatcatt gtgctttttg ctcttgtatt caacaattct
     4381 ttaggctggt tcttgttggc cttttttact ctttatgtat catggagctt gatttcatta
     4441 agcagctttc taaagaaact ttcggttact tgtacattgc agccactcgt ttatcgcaaa
     4501 aaaagtatgc aggttatttt ttccatagca aaaaagagaa ctttttcttt acctctccct
     4561 cgtttacaaa tcggcttccc agcaatcttt gcagaaaaaa acaagagatt ttgattctta
     4621 caaataaacc gcaagaagtc caattgcttt ggcagccaaa agaaagagga tgccatgaaa
     4681 cattaactgt catttttaca gcctatgata gtctaggact tttcagaaaa agtgcaaaaa
     4741 gagagatctc ttttcctgtg actgttctgc ctgctttttg taagattcaa gcagaaaaat
     4801 tacagagagt tctggaaaaa aaacagcagc tgaacgcgta ccaaagaggt cttgattttc
     4861 gggaacatcg cgcttatcgt ccaggtgatt ctttcaaccg aatcgattgg aaattgtctg
     4921 ctcatactca agagtggtta taccgtgaat acgattatca agaagaagaa tgttctcctc
     4981 ttttttgctt ttggggtttg cccagtccaa aatttgaacc tctgctagac ttgtattttt
     5041 ctttatggca tttgagaaaa gaaaagcagc cggaacttct gatattaggc aaccgcgcct
     5101 ttcatggcaa agaccccggt catacctatt ttgcttcttt ggaagctggc gatgagaaag
     5161 cttttttcgc acacttgaaa aaatatgcaa aaaaacaaat cgtcctcttt gttcctgaga
     5221 attctcctga agtctcagaa gctattgaaa cattgtcaaa agatcgaacg gtttatcttg
     5281 tttatttcgt agaaaaacaa ctcatggtcc aagaaaaaga caagatatgt tcattgccag
     5341 gaggtgaatg gattgacgat taaacgggaa aaatggctgc gtggcgttta ctcctttgtg
     5401 atactaagtg taggtgctcc gatatttgta gtagctaatc aattgcaaaa cagctacctt
     5461 cttatcctaa ctattggtgt cctatgcctt attgtctgct gtattcctaa aaaatgggta
     5521 cgtttgctgc tttctttgcc agtaatgata ggttgtttgt ttttatattt tccttcgaag
     5581 acattttcca tattatggat cttacagttt ttttcgtcag tcacagagga attcttccat
     5641 ctatcccaag gtaaacttta ttatttgcca gaaaagacag ctttttttct cataatgttg
     5701 ctgatctatt tatttattac attgaccgtg gattacgact attggtacgc accctttctt
     5761 tctttgatgg cttacttcat gatgcttacc gtttttcgga aaaaagattt actgtacgaa
     5821 atgattgcgg tagtaactgt tttgttcgtg tatctggcag caaggcaatt tttctccatc
     5881 cggttgttgt cgggaaacac tcgattccag tcattgacta cagttctgct tatttgtttc
     5941 ttaactttta gcgctatctt gcctttatat cttccgaaag tacaccaatc tctcgaggaa
     6001 acgagtgaac cgatccgaga atatcttaat gatgaagggc tttatgacac actccatgag
     6061 tatcaattaa ccggaagtgc aagaacaggc ttcagtgaga atgatgaaca gcttggtgga
     6121 cctttatatg ataatggcga agttgttttt acagcgaacc aaaaaggaaa tggctattgg
     6181 aagatcgaaa ataaaaatca ttacaccggt tctggatgga tagaagatca ggacgcaccc
     6241 atccaaccaa tccaagaatt ccctgttgac atcacgagct accaaaccgt tatagaagac
     6301 aaagaaaaca ttacattgac cctaaaaaaa caagttttct taccttatcc ttatggaaat
     6361 actaccttga ctgatttttc attagctagt ggacaaacgc tttactattt gcctgaaagc
     6421 cagcgattca ttcctgtccc gaatgaatcg acacgttctg caactttaca aacagaaagg
     6481 ttagttttta cgagagaaga attaaaaagc agtcagttaa aagctgatga tttagaaaat
     6541 cagcagacaa atcttcaatt gccaaaaagt ttgcctaaac gtattgagga cttggcggtc
     6601 gaattgacca aaaatgaagc aactttaatt cgtaaagtcg aagctgtcta acagtattta
     6661 aaaacaagcg gaaatttccg ctattccaaa acggataccg ggcatactcc aaaagaacac
     6721 gattatgtgg attatttcct ctttgattca aaagtcggct attgtgacaa cttttcaact
     6781 tctatggtcg tccttcttcg aacacttggt atcccagctc gctggacaaa aggttttaca
     6841 aatgggctca gaacaggcac agataatcaa gggaatgagg aatacagcat tctcaatagt
     6901 catgctcatt cttggccaga ggtatacttt cctggatttg gctggcttcc atttgaacca
     6961 accccttcat ttgccaatcc aatgggttca caaccagttg ctgaaacaac cgaaaccgtc
     7021 cagcctgcta tcgaacaacc aaatgaaagc gaaaatatgg cagaaactat ttccagtgag
     7081 agatctgagg tcattccatc agaagaaaca acactcacac cagcttataa aatcgtctta
     7141 ttcagccttg gagtgtgttt atccgtcttg ttgttctttc tattgttatt cagaaaatct
     7201 ctgcgctgga caagttatct tatctatctt catttttttg cttctactaa tacgattgca
     7261 tatcaaaaac ttcttaaccc tttggaaaca ttggtaccgc gcaaacccgc agaatctttg
     7321 caggaatata gcctgcgaat agaacaagaa ttcaagctag atctgcgttt cattcgatta
     7381 actactcaat atgaagcaga aatatatgga cgacaagcag atccggtaaa attcgataaa
     7441 aaaacctatt tagaaataac gcatataata agaaaaatgg tatacaaaaa aattctgaca
     7501 aaagggagcc gcttcaaaaa ataagataaa atttctggag aatctacttc tggaatgccg
     7561 agaaccagct gattgagtat cgattactcg aaaaaaaggt tgtgacacgt tttttgtcac
     7621 aacctttttt cattattatc tgattttcaa tgtcaaagca tttagagcta caactaccgt
     7681 acttaacgac atcaagacag caccaactgc tggacttaat atgataccaa ttggcgcaag
     7741 aattccggct gctagcggaa ttgcaagaat attgtaacct gccccccacc agaggttctg
     7801 gatcattttc tttcgcgttt gcttggctag atcaagaaaa tgcaaaatat cttttggatc
     7861 gctgtttgtc aatacgacat ccgcagaatc gattgcgata tctgtccctg caccgattgc
     7921 catcccgatt gaagcacggg ctaaacttgg cgcatcattg atcccatcac ccaccatgat
     7981 tacttttttc ccttggtcaa ggtatttctg gacaacagct tctttatcat ctggtaataa
     8041 accgccataa tattcttcga tccctaaata atctgctact gcttgggcag cgtttttatt
     8101 gtcaccagta agcatgacag gagtgatttt tctttctttc aatgttcgga taaattcttt
     8161 tgcttctgtt tttactacgt cacctaaagc aacgattgca gctagatgat tttctaaaat
     8221 caggaagctg atggtatttc cttgttcttg gtaaggtttc aataatgctt gttctacgtt
     8281 taatccgaga cgctcaactt ctttttcatt aactattttc acttgttgtt ttttgactgt
     8341 tgcctctagc ccgactcctg aaagattttt tagattttca gcttgataag gcttgatttc
     8401 atgttctttc aaataattca taatgccaac agccaatgga tgattggcat tcgcttccaa
     8461 ggctcctaga tattgaagga tttcattttt attgaacgca tcatcaagta cttcgacacc
     8521 cgttacagta aattttcctt ctgttagtgt tcctgttttg tccagcatga tcacatctag
     8581 atcattggct tgctccagtg cattgcggtt tttcaaaagt aatccgtttt tagctgctat
     8641 tgatgtggaa cgagcaacaa ctaatgggat agctaaacct agagcatgag gacaggcgat
     8701 gataaagact gttaccatac gttctaatgc attaggaaga tctgtcaaga acaaccaggc
     8761 gatgaatgcc aggataccca caattaatgc gacataaaac agccattttg ccactttgtc
     8821 agataatgac tctagcttgg atttttctcc ttgtgctttt cgcaccattt ccataacctt
     8881 cgctagataa ccattttcac cagttccagt tacttcgatt tcgatcgttc catccccatt
     8941 gatagaaccg ccaatcactt tgtcattggc ttgttttttg attcctcttg attcgccagt
     9001 cacagctgat tcgtcaacaa ttgtttcacc tttcaagatt acaccatctg ttggcatttt
     9061 atcaccagac cggacgatca aacgatcacc ttcgtttact tctttcaatg aaactgtctc
     9121 ttcttttcca tctttcgtca agcgtttcac tgattctggc agtaattctg ctagtttttg
     9181 taaagcattg ctggcattcg acacagcatt catttcaatc cagtgaccta gtagcataat
     9241 aacgatcaaa gtagccaatt cccagaagaa atccatgacg tgtgtatgtg gattgagcag
     9301 atttgcgata aacgaataga cactataaat atatgcaact gtaatcccca tcgcgatcag
     9361 tgtcatcata gcaggacttt tttgtttcaa ttccatctta gctccactta aaaatggctg
     9421 accgccatag ataaatagta tagttgctaa gatcaatacg agccaatcag aaccaggaaa
     9481 cgttacttga aatggaaatt ccattcccat cattggtgaa aaaagaatga ttgggatagc
     9541 taggataagc gatagccaaa atttttgttt gaaattcccc atgtgcatgg aatgatccat
     9601 gccgccgtgt tcgtgatcca tgtgcatcga atggtccatt tgtttgctgt ttgctatatc
     9661 tccatggtta tgtcccatgt gcatggaatg atccatctga ctatggtcat gtttcatctc
     9721 catctgtttt tcatgagatt ctgtactttc tttgtttact acttcttttt tatgctcatg
     9781 ttttttctcc atatcgttcc cattcatttt tactccatcc cttctcttac tttttcatgt
     9841 gtcgatttaa gcacagggag ttcaacaata cactgacgga actaaacgcc atcgctcctc
     9901 ctgcaatgat tggattcaaa aagcctagtg cggcaaaagg aataccaatc gtattataga
     9961 caaaagccca gaaaagattt tgtttgattt ttctcaatgt caacctagac aactcgattg
    10021 tttgtgcaat ggatgccaaa ctgctattca tcagcgtcac atcggctgtt tccatcgcaa
    10081 tgtccgttcc acttcccatt gccataccga catcagctaa tgctaaggcc ggtgcatcat
    10141 tgattccatc gcctgccata ccgacttttt tgccatcttt ctgaagtttt tctacgtagg
    10201 ctgctttatc ctctggaagt acttcagcaa agatatgctt tggatcaata ccaacttgtt
    10261 taccaatcgt ttcggctgcc agtttgttgt ctcctgtcag cataaaaaca tctaatcctt
    10321 tattctggag ctgcttgatt gcttgtttga cttctagttt gatttgatca gccacggcaa
    10381 tcaacccgat cacttgcttt tcatcagcta aaaacatgac tgtttttccc tgctgttcta
    10441 attctaacgc gtgttcttgg tattcatcaa atgataggtt caattcaatt aatcgtttac
    10501 gtgtacccgc aaaatagcgt actccgttaa tcgtaccgct gattccagca cctgggtggg
    10561 ctgtaaaatc aatcatatcg tatgttgctg cttcttgcag ttttccgtat tccacgattg
    10621 ctttacctag tggatgttca gaagcttgtt ctaaagtata aaaaatagat aggacttctt
    10681 tagaaccgac tagatctgta actttgggct ttccttctgt aatcgttcca gttttatcca
    10741 aaacaatgct gtctaagtga gcagctgctt ctaatgcttc tccgccttta attaaaattc
    10801 catttcgtgc accaacaccc gttcctacca taatagctgt tggagtcgct aaacctaatg
    10861 cacatggaca cgcaatgact aatacagaga cactatggat caatgcgagt tgccaatcac
    10921 cagtgatcaa acctgtagct atgagcgtga taaacgcgat gactaatacg atcggaacaa
    10981 aaatccctga gatccgatct gcgatttttt ggattggggc tttgctccct tgagcatctt
    11041 ccaccatttg aatgatttgc gctaatactg tctgcttgcc gacttgtgta acttccatgt
    11101 gaagtagacc attcgtattg attgtcccgc caaagagcgt gtcatccgga ttcttttcta
    11161 caggaagatt ttcaccagta agcatacttt catctatcgc actgcttcct gaaataattc
    11221 gaccatctgc tggaatctgc tcaccaggac ggatgactag ttgatcacca acgactactt
    11281 cttcaatcgc taacgtttct tctttcccgt ttcttatcac ttgggcggtt tttgtttgaa
    11341 gagacatcaa ttgtttgatt gcattacctg ttttcgtttt tgctgtatgt tccaaatatt
    11401 tgccgagcaa aatcagcgtg atgatcatac tgctgctttc aaaataaaga tcttgggggt
    11461 gtccacggaa aaatccgttg taaatgctca atgcaaaggc agccgatgtt ccaattgcta
    11521 ccaatacatc catatttggt gctttcgtct tgattgcatg gtacgcacct ttataaaaac
    11581 gagcaccgac ataaaattgg acaggagcag acagcactaa ctggacgatt ggaaaatgaa
    11641 aaaagtgaac aatcgccgca tggcttccta acatcatagc aatcattgac aacatcaacg
    11701 gcaaagtcaa aaccgtactc aatataagat cgaatagcat ttttttcaaa tacgcttgct
    11761 tttcttctgc aattttttgt ttgtgggctt catcgtacaa aattgcacca tagccgatgt
    11821 tttctacgct ttggattaat ttttcagtgg tcgtcccttc atatttgaca cttgcttttt
    11881 cagtggccaa gttgactgtg gcattgacca caccaggttg tccgttcagc tctttttcga
    11941 tcctagcaga acaatttgca caagtcatac ctgtaatgac gaacgtatcc attttggttt
    12001 ctgctgttgc catcaaatca cctctgcttt ataacccaat ccattgattg cttgacaaat
    12061 cttttcggat tgaacttttg tttcatcaaa cttgaccgta ccgtttgctt ttttcaaatt
    12121 tactttcact ttttgcacgc cgtcaagtgc gcttactgtt tcttctactc ttgctgcaca
    12181 atgattgcag ctcatacctt cgattgaaaa ttgttgtttc attttttctt cctcctattg
    12241 atgttgttta cattcacatt gacctgggat acagttacat tcgatcgtat ctactggttg
    12301 cttttgttcc agttgtttca aaatctcatt gatatcttct tgagtcaaag tggcttcttc
    12361 tactagatcc gcaatcgtct ctccaatcct ttttgcacag atatgggaaa aaagattttc
    12421 cgtcgcactg cgaactgttt ccatctccga gacagctgga taataaatga atttttttcc
    12481 ttcttgttct gtccagatca cttctttttt taccaaacga cccagcaaag ttttgatcgt
    12541 tgctggtttc caatccatcg aatcagctaa gatctgcgtg atttcttggg ctgtcgtctg
    12601 tccaagtgtc caaataacac gcatgatctc ccactctgaa tcactgatct ttactggcgt
    12661 tttgactttt tccatttatt tcacaccttt cgtttacaaa tgtaatcatt atcttatgaa
    12721 tacagattac acttgtaaac aaaaactgtc aattattttt gctctctatt ttcaatttag
    12781 ttaaaaattc ttgtttctct cgtcttgttt taaggatgat cagtttattt cttgcattag
    12841 ctacttggat cagctgctgt attttcggtt cattgttttc ttcaaaatgc cagataaaag
    12901 ataaaaattc taagtattcc ctgtttagtt tctccgaaaa tgattcaggc atatctggcc
    12961 gagatttttt gtttatcttt ctttttagag aacgccaaac tactcggtag atggcgagat
    13021 aacgcggcac tttaagccaa atgatcttat ctgcttcttt aatcctttcc tctaatgtag
    13081 aagtataatt tccttcaacg atccatgacg gattagatac cataaacaat tgctgttctt
    13141 gcaaaaacca cttttttgcc tgaatactgt agtctgttgt atgccataaa aggtctagcg
    13201 gcagtagcgg aaagtcggtg atttctttga tcttccttgc gagggtcgat tttcccgacc
    13261 cactttgtcc aatcacgatg atcttcattc ttctccctca cttctctttg gaatttggtt
    13321 cgatcatacc acttataacg ccgcaaaaaa acaagggact gtgacaaaag tcgtgtcaaa
    13381 gtcccttgtt tcgaataa
//
LOCUS       DACDKM010000077        13154 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20834_2016
            SAMN12640370-rid9891663.denovo.077, whole genome shotgun sequence.
ACCESSION   DACDKM010000077 DACDKM010000000
VERSION     DACDKM010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640370
            Sequence Read Archive: SRR10040891
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13154)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13154)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742835.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742835.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:04:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,012
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 29 of 75
            Pseudo Genes (incomplete)         :: 33 of 75
            Pseudo Genes (internal stop)      :: 19 of 75
            Pseudo Genes (multiple problems)  :: 5 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13154
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640370-rid9891663.denovo.077"
                     /isolate="E20834_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..267
                     /locus_tag="IV334_001994"
     CDS             <1..267
                     /locus_tag="IV334_001994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8944053.1"
                     /translation="LKQFPQEEHFMYKEAISHKVTEYLEHNFFRIKPVRTRSAKGIYE
                     MKVKINKKNYRIAFALKKNHVFIFYISSNLQKIVFDKEVSKKIK"
     gene            complement(364..798)
                     /locus_tag="IV334_001995"
     CDS             complement(364..798)
                     /locus_tag="IV334_001995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP8944054.1"
                     /translation="MNIDSLMIDEISELYKSVGWTNYTKDTARLARAFEQSESLIKRN
                     GEGKIIGIVRWITDFATIAFIQDILIHPRYQRQGIGKVLLNEALEKITSYGPVQIELL
                     TDDTEKTKKFYESVGFVQVKEMGGISYVIDTRRKKVLFGNDK"
     gene            complement(1030..1740)
                     /locus_tag="IV334_001996"
     CDS             complement(1030..1740)
                     /locus_tag="IV334_001996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010780519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HAP8944055.1"
                     /translation="MSCEEKIAAMKQRVLEANLALPRYGLVKLTWGNVSEVDRELGVI
                     VIKPSGVRYECMQADQMVVTDLSGNIIEEDSLKPSSDLPTHVVLYQTFEDITAITHTH
                     STHSVMWAQAGRDLPAYGTTHADAFYGKVPCTRQLTKEEVREAYEVHTGNVIVETFKE
                     RKLDPNEVPGVLVYGHGPFTWGDSPMKAVENSLILDEICLMAKENELINPNICEIPQY
                     LLDKHYFRKHGKYAYYGQ"
     gene            complement(1730..2590)
                     /locus_tag="IV334_001997"
     CDS             complement(1730..2590)
                     /locus_tag="IV334_001997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 3-epimerase"
                     /protein_id="HAP8944056.1"
                     /translation="MAQIGIYEKALPKDISWKERFSLVKEMDFDFIEMSIDETDERLA
                     RLDWSEEKIAELREEMFSSGVRIHSICLSAHRRFPFGSADPEKKKAAKQLMKKAIHLA
                     HNLSVKVIQIAGYDVYYEEKSMNSREDFIEGIKESVKEASKYGIILSVEIMDDPFMNS
                     ISKFLEIKEQIHSPFLQVYPDLGNLSAWPENNPARELEKGIDYITAIHLKDTYPVTKE
                     YSGQFRDVPFGSGCVDFLGLLRHLKRLDYDGTFLIEMWSEKSQDFRREIQQAKDYLSS
                     KLKEAGYDVL"
     gene            complement(2595..3233)
                     /locus_tag="IV334_001998"
     CDS             complement(2595..3233)
                     /locus_tag="IV334_001998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010740138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-L-gulonate-6-phosphate decarboxylase
                     UlaD"
                     /protein_id="HAP8944057.1"
                     /translation="MSRPNLQIALDHNDLEHALGDVMKVGDVVDIIEVGTILCLQEGQ
                     KAIRCIRSMFPDKKLVADTKCADAGGTVASNVAKAGADWMTVICCATIPTMEAAQKEI
                     GELQVELYGNWTFEQAMDWHNIGIRQVIYHQSRDALLSGETWGEKDLSKIKKLIELGF
                     NVSVTGGLNPHTLHLFEGIDVYTFITGRGITAANDPMKAAQNFKDEIIRIWG"
     gene            complement(3248..3547)
                     /locus_tag="IV334_001999"
     CDS             complement(3248..3547)
                     /locus_tag="IV334_001999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAP8944058.1"
                     /translation="MRILVSCANGSGTSLMMMRSVEKAMKELNVPITKIHHCAISEGK
                     SSAGQYDVVFTPVNFLNMFTQAEKKGVTVIGIKNVMSVKEISEKFAETKWYQKNK"
     gene            complement(3590..5074)
                     /locus_tag="IV334_002000"
     CDS             complement(3590..5074)
                     /locus_tag="IV334_002000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HAP8944059.1"
                     /translation="MGEFLLSIWTYFATNILTQPAYLIGFIVLLGYILLKRPLHECIA
                     GFLKATVGYFILTVGSGGLVNNFRPILVGLKERFNLDAMVTDPYFGQNAVDAGLMETF
                     GRTFGDVMILLLIAFVLNILLVRFQKYTKLRSVFTTGNVQVQQAATAFWILLFCFPDL
                     GRIQALIFMGLILGCYWTVASNLTVGITQELTEGAGFAVAHQQMFGIFIFAKLAEWMK
                     KRDEKKNRSIKQDKKIEDIKLPGFLSIFNENMVATSLLMLFFFGIILIVLGKDYLIQA
                     QFMQEGQSFLFYIMTTSLNFAVYLAILQLGVRTFVDELTQSFQGISNTILPGAVPGID
                     VAATFGFGSPNAVTIGFLFGALGQFLMIGLLILLKSPTIVIAGFIPLFFDNAVIAVYA
                     NNRGGFKAACLFPFLSGIIQVGGSALFATWIGMSTYGGYIGMFDWATAWPLFTVIMKV
                     LGYAGIAVVVIGLLAIPQLQYRKNPDGYFLIVEDYDTYAEKQRV"
     gene            complement(5094..5555)
                     /locus_tag="IV334_002001"
     CDS             complement(5094..5555)
                     /locus_tag="IV334_002001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAP8944060.1"
                     /translation="MLKYFFDHQLIHYPDKEITDWREAIAESCLVLIDKEIIDETYVE
                     EIIHCVEKYGPYIVIAPQVAMPHSSEKSDGVFGTAISFTKLKQPIRFESEGENEEKTA
                     SLFFTLAAKDPEEHLENIQKLSELLMTDGLVERLISTNSIDDYQVVMAEYN"
     gene            complement(5573..6640)
                     /gene="ulaG"
                     /locus_tag="IV334_002002"
     CDS             complement(5573..6640)
                     /gene="ulaG"
                     /locus_tag="IV334_002002"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ascorbate 6-phosphate lactonase"
                     /protein_id="HAP8944061.1"
                     /translation="MSKTVNEVTKEKWLVETFPEWGTYLNEEIETEVVDPGTFAMWWL
                     GCTGIWLKSHEGTNILCDLWCGTGKRSHGNGMMKKGHQMMRMSGVQNMQPNLRTQPFV
                     IDPFAIKNVDALIVTHIHSDHLDIHTAAAVHQNCPEATFIGPKEVVRTWQKWGIPEEK
                     TITVKPGDAVKIKDINVVALEAFDRTALVTCEDPEEVLKGKMPQEMDQIAVNYLFETS
                     GGNLYHAGDSHYSNLFAKHGNEHKIDVCLGAYGENPRGITDKVTSVDMLRMAESLNAK
                     VVIPVHYDIWSNFMADPKEITEIWKFKKDRLKYGFKPYIWQVGGKFIYPTNKDDLEFN
                     FYRGFEDVFSIENDTPFPSFL"
     gene            6858..7628
                     /locus_tag="IV334_002003"
     CDS             6858..7628
                     /locus_tag="IV334_002003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HAP8944062.1"
                     /translation="MKNSLVNIEKRQKEILELLQQTEQLSTFELAESLDVSISTIRRD
                     LNLLEEKNEIIRNYGYCSYNYKNQTDFDQSGPERIKQAIAREASKYINDYDTLFINSS
                     STALKTLDFLQSNHLTVITNNVKINYSPHKDNCSYILTGGEMRFPKEALVGDIALNTI
                     SSVNADICIIGCSGVDVVHGVTTKILNESTINEMMVNKTIKCKILVADHRKIGLTSKF
                     KIADISVFDYLITDKFCSEKVLEEIRNTGIKTIQVDSF"
     gene            complement(7901..8728)
                     /locus_tag="IV334_002004"
     CDS             complement(7901..8728)
                     /locus_tag="IV334_002004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP8944063.1"
                     /translation="MIKVDGALLKEYRKKKGLTQVQLAEGICEQATVSNIENKNMSPG
                     INILSALCKKLNVEISDILVETEEDKIRKKINEIQSLNSVSKYQESYKILQTIDQEKV
                     SNSDLLNQILFYQGLLDHLYAKKPDAALFYFNQVLEQTTETDIHHLQAAANVAMIYLS
                     KGELDFAKVYVERTLKILSETDFDNLMVCIVYYDIATYYRKIEDYDKAIQLCEKGIEY
                     NKKHKSTYALEYLLYEIASCHKQLGEDDYLERYMDAKKIARFNGNDYAVKVIENDLK"
     gene            9296..10531
                     /locus_tag="IV334_002005"
     CDS             9296..10531
                     /locus_tag="IV334_002005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="HAP8944064.1"
                     /translation="MKTILFAPAVFNLAETTRMIEVAKAMKDRYYCVFFGFSKTFAPL
                     ITESGFEYHLLAPILTKKQEEQIIKFDQLRTLKNPFTKEMVASRITSEQSLIAKFEPR
                     LIVTGSNVTIFLSARNKKIPLVYVKPYALSEPFFQSEHALMPHELRFFGPLSNPLWHW
                     TKKRIVNTRWIPNAFKHAAQKEMLPLPKTSLQMMDADLNLITTPKLFLEEEQLPENYR
                     IVGPIFAKLSTSLPEEVLTFIQRKQQERKRIIYFAMGSSGNPRMLRKILFFLQKQPDL
                     AVIAPIDYLLDDIQSDSEDLLITEYLSSHLLHEFIDFSFIHGGEGTVQTACESGKPFI
                     GVGMQFEQSCNIDYCVKYGNAIALTKPVTERKLQIALREVSSPKIRQQARTLQAVFPI
                     NGPQQAIQEIETFLSNHPI"
     gene            complement(11530..11790)
                     /locus_tag="IV334_002006"
     CDS             complement(11530..11790)
                     /locus_tag="IV334_002006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HAP8944065.1"
                     /translation="MYWLILFFVFIFLLTASHLILNMLATYHIQINRWIWALASFLIV
                     ILPKIIVPHMNVLFSWGTYVLCGIFAINFMIEQHRWFVTSKL"
     gene            complement(11817..13118)
                     /locus_tag="IV334_002007"
     CDS             complement(11817..13118)
                     /locus_tag="IV334_002007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAP8944066.1"
                     /translation="MEKLMKWVEEKLVPPMAKIGTQRHLLAIRNGVVSTLSLILIGTF
                     FMVFINLPFPGWNEFIAPYSATIVLPFRMTMGLMAIYATFVMGSDLAKSYGLDSVTGG
                     ILSLGTFFMLQVPVNVLTPEEAPLGWVLPMSSLGASGMFAGILSMIFAVEVYRFFKNR
                     NITIKMPEQVPPAVARSFEALIPGAVILTITWLIRSVAGFDVNAALLSLFEPLTNILG
                     NNLLGVLLPMFLIHLLWSFGIHGMSIVGAVVRPMWLIMLDENAKALADGTAATQLPYI
                     APEQFYQWTVTIGGAGATIVVSVLFIFFCKSKFLKEVGRFSIIPGIFNINEPIIFGAP
                     MMLNPYMFIPFNLVPLVLTIISYFAVKLEMVNRFTVLPTWTLPAPIGGYLSAGNDWRV
                     VILIMINTLIAFIIYYPFVKAYDKKMLSDEQSRGENEVQTV"
BASE COUNT         4165 a         2749 c         2069 g         4171 t
ORIGIN      
        1 cttaagcaat ttccacaaga agaacatttc atgtataaag aagcaatttc ccacaaagtg
       61 acagagtatt tggagcataa tttttttcgt ataaagccag tgcgtactcg ttcggcaaaa
      121 ggaatatacg agatgaaagt caaaattaat aaaaaaaatt atcgcatagc atttgcttta
      181 aaaaaaaatc atgtgttcat tttttatatt agttcgaatt tgcaaaaaat tgtatttgat
      241 aaagaggtga gtaaaaaaat aaaatagctg agcattggag gatagattga gtgatactaa
      301 gtctatcctc ttgtttttct aaagaactaa gctagaattt tttcttttgt acgttattat
      361 atgttatttg tcattaccaa aaagcacctt ttttcttcta gtatctatga cataagaaat
      421 accacccatt tcctttactt gaacaaatcc tactgattca taaaattttt ttgttttctc
      481 agtatcatct gtcaataatt cgatttggac tggtccataa ctagtgatct tctccaaagc
      541 ttcattcaaa agcactttgc ctattccttg gcgctgatac ctcggatgaa tgaggatatc
      601 ttgaataaat gcgatcgtcg caaaatcagt gatccaacga acgattccga taatcttccc
      661 ttccccatta cgctttatca gagattcaga ttgttcaaat gctctagcta accttgcagt
      721 gtcttttgtg taattcgtcc aaccaacgct cttatataat tcagaaatct catcgatcat
      781 aagggaatca atattcattt ttttcttcct ttcttataca attctccctc taataataca
      841 gttctttgac tatttaatct atcacatata actctcctgt aaagattctc tcgattcctt
      901 aaaattcctc tttcctttta gaatgcatac tttttgataa ataaaaaggt tgtgataaat
      961 tttcttcaca acctctgttt ctctctatca agtgttatct tttaattttc tacccttacc
     1021 ctagcatctc tattgcccgt agtatgcata ctttccatgt ttgcgaaaat aatgtttatc
     1081 tagtaaatat tgcgggattt cacatatatt ggggttgatc agctcgtttt ctttggccat
     1141 cagacaaatt tcatctaaga tcaaactgtt ttctactgct ttcataggtg aatcccccca
     1201 tgtgaacggt ccatgtccat agacaagtac tccagggact tcgtttggat ctaattttct
     1261 ttccttaaac gtctcgacaa tcacattccc tgtatgaact tcgtaagctt ctcttacttc
     1321 ttctttcgtt aattggcgtg tacaaggaac cttaccataa aaagcatcag catgagtcgt
     1381 gccataagct ggcaaatcgc gtcctgcttg cgcccacatg acggagtgcg tggaatgagt
     1441 atgagtaata gcagttatat cttcaaaagt ttgatacaaa acaacatgtg tggggagatc
     1501 tgaagatggt tttaagctat cttcttctat tatatttccc gacaaatctg ttaccaccat
     1561 ttgatctgct tgcatgcatt catatctgac accgcttggc ttaatgacga tgactcctag
     1621 ctcacggtcc acttcactca cattgcccca agtcaatttg actaatccgt atcgcggcaa
     1681 tgccagattt gcttcaagga cacgttgctt cattgctgca attttttctt cacaagacat
     1741 catagccagc ctccttcaat ttagaactta agtagtcttt tgcctgctgg atttctcttc
     1801 gaaagtcttg gcttttttca ctccacattt caatcagaaa tgttccatca taatctagac
     1861 gtttcaaatg tcttaataat ccaagaaaat caacacaacc gctaccaaaa ggaacatcac
     1921 gaaactggcc tgaatattct tttgttacag ggtaagtatc ttttaagtgg atagcagtga
     1981 tataatcgat ccctttctca agttctcgag ccggattatt ttccggccat gcggacaaat
     2041 tgcctaggtc tggatatact tgtagaaaag gtgaatgaat ctgttctttg atttctaaaa
     2101 attttgaaat ggagttcata aatggatcat ccatgatttc cacggaaaga atgatgccat
     2161 atttagaagc ttcctttacg ctttctttta ttccttcgat aaagtcttct ctggaattca
     2221 ttgatttctc ttcataataa acatcgtatc cagctatttg gattactttg acgcttagat
     2281 tatgcgccaa atggattgct tttttcatta gttgtttcgc tgcttttttc ttttctggat
     2341 cagcagaacc aaacggaaat cgccgatgcg cacttaaaca aatcgaatgg atacgtacac
     2401 cggagctgaa catctcttct ctcagctcag ctatcttttc ttcagaccaa tctaatcgtg
     2461 ctaaccgctc atccgtctca tcaatcgaca tctcgataaa atcgaagtcc atttctttta
     2521 caagggaaaa acgttctttc catgaaatat ctttgggtaa cgctttttca taaatcccaa
     2581 tttgagccat ttccttaccc ccagatacga atgatctcat ctttgaagtt ttgtgcggcc
     2641 ttcatcggat catttgctgc tgtaatacca cgaccagtga taaaggtata cacatcgatc
     2701 ccttcaaata agtgtaatgt gtgtggattt aatccaccag ttacagaaac attaaagcct
     2761 agttcaatca gttttttgat cttcgataaa tctttttcac cccaagtttc accagataac
     2821 agcgcatcac ggctttgatg gtagattacc tgccggatac cgatgttatg ccaatccatg
     2881 gcttgttcaa aagtccaatt gccgtagagt tctacttgca attcacctat ttctttttgt
     2941 gctgcctcca tagttggaat cgttgcacaa caaatgactg tcatccagtc tgctcctgct
     3001 tttgcaacat tactcgccac tgttcctcca gcgtctgcac atttcgtatc agcgactagt
     3061 tttttatctg ggaacatact gcgaatacag cgaatcgctt tttgtccttc ctgtaagcaa
     3121 agaattgtgc ccacttcaat aatatctact acatcaccaa ctttcataac atctcctaat
     3181 gcatgttcta agtcattgtg atctaacgca atttgcaaat tcggcctgct catcttagtt
     3241 ccttccttta cttgtttttt tggtaccact ttgtttcagc aaatttttca ctgatttctt
     3301 tgactgacat cacatttttg ataccgataa ctgtaactcc ttttttctct gcctgagtaa
     3361 acatattcaa aaaattgact ggcgtaaaaa ctacatcgta ttggccagct gaactttttc
     3421 cctctgaaat ggcacaatga tggattttgg taattggtac gtttagttct ttcattgctt
     3481 tttccacact tctcatcatc atcaagcttg ttcctgatcc atttgcacac gatactaaaa
     3541 ttctcatctt aatccctcca ttatgagtgt tgtctaatca gattttcttt tatactctct
     3601 gcttttctgc atatgtgtca tagtcttcta caatcaaaaa ataaccatca ggatttttcc
     3661 gatattgtaa ctgcggaata gccaataaac caatgacaac tacggcaatt ccagcataac
     3721 ctaggacttt catgattact gtaaacaatg gccaagctgt tgcccagtcg aacatcccga
     3781 tataaccacc gtatgttgac atcccaatcc atgtagcgaa taatgctgaa ccgcctactt
     3841 gtatgattcc tgaaagaaac gggaacaggc aagcggcttt gaatccacct cggttattgg
     3901 cataaactgc tataacggca ttatcaaaga acaacggaat gaaaccggct atgacgatcg
     3961 ttggtgattt caataaaatc agtaaaccaa tcatcaaaaa ctgtcccaaa gcaccaaaca
     4021 agaacccaat cgttaccgca tttggcgaac caaagccaaa agtagcagct acgtcaattc
     4081 ctggaacagc tcctggtaaa atcgtgtttg agattccttg gaaagattga gttagttcat
     4141 cgacgaatgt ccgaacacct aattgcaaga tcgccagata taccgcaaaa tttaaagaag
     4201 tcgtcataat gtagaataag aaactctgcc cttcttgcat gaattgcgct tgaatcaaat
     4261 aatctttgcc tagtacaatc agaatgatcc caaagaaaaa tagcatgagt aacgaagtag
     4321 caaccatatt ctcattgaaa atcgacaaga atcctggtaa tttgatatct tcaatttttt
     4381 tgtcttgctt gattgatcta tttttctttt catcccgctt tttcatccac tcagctaatt
     4441 ttgcaaatat aaagattccg aacatttgct gatgtgccac tgcaaatccg gcaccttctg
     4501 tcaattcctg cgtgattcct actgttaagt tagaagctac tgtccaataa caacctaaaa
     4561 tcagtcccat aaaaatcaat gcttgtatcc tacctagatc aggaaagcaa aacagtaaaa
     4621 tccaaaaagc ggtagctgct tgttgaacct gaacgtttcc ggtagtgaaa acggaacgca
     4681 gtttcgtata tttctgaaaa cggactaata agatatttaa aacaaatgca atcagtaata
     4741 aaatcatcac atctccaaag gtacggccaa atgtctccat taatccagca tccacagcat
     4801 tttgtccaaa ataaggatct gtaaccatgg catccaaatt gaaccgttcc tttagtccaa
     4861 ccaagattgg ccgaaaatta ttgaccaaac caccagagcc aacagtcaaa ataaagtaac
     4921 cgaccgttgc ctttaagaat ccagcgatgc attcatgaag cgggcgtttc agcaaaatat
     4981 agcctaataa cacgataaaa ccaatcaggt atgctggctg cgtcaagata tttgttgcaa
     5041 aatacgtcca aatgcttaac aaaaattccc ccatcttttt tctcctctat caattaatta
     5101 tattctgcca tcacaacttg gtaatcatcg atggagttcg tagagatgag acgctcaacc
     5161 aatccatctg tcattaataa ttcggataat ttttggatat tttctagatg ttcttctgga
     5221 tcttttgctg cgagtgtaaa aaacaaacta gccgttttct cctcattctc cccttcactt
     5281 tcaaaacgga ttggctgttt caacttggta aatgaaatag ctgttccaaa aacaccatca
     5341 cttttttctg aagaatgcgg cattgctact tgaggtgcga tcacaatata aggaccgtat
     5401 ttttctacac aatggatgat ctcctccaca tacgtttcat caataatttc cttatcgatc
     5461 aatacaagac agctttcggc gatagcttca cgccaatccg tgatttcttt atctgggtaa
     5521 tgaatcaatt gatggtcaaa aaagtatttc agcaagaaac tccccttctt tcttataaaa
     5581 acgacggaaa cggtgtatca ttttcaatcg agaagacatc ttcaaatcct cgataaaaat
     5641 taaactcaag atcatctttg tttgtcggat agatgaattt cccgcctact tgccagatat
     5701 aaggtttaaa tccatacttc aggcgatcct ttttgaattt ccaaatttca gtgatctctt
     5761 ttggatcagc cataaaatta gaccaaatat cgtagtggac aggaatcact acttttgcat
     5821 tcaatgactc cgccatgcgc agcatatcaa cagaagtaac tttgtctgtt atccctcgtg
     5881 ggttttcacc ataggcccct aaacagacat cgattttgtg ttcattacca tgtttggcaa
     5941 ataaattgga gtaatgtgaa tctcctgcat gataaagatt ccctcctgat gtttcgaata
     6001 aatagtttac tgcaatttga tccatctcct gaggcatttt tccttttaat acctcttctg
     6061 gatcttcaca agtgaccaac gctgtccgat caaacgcttc taaagctact acattgatgt
     6121 ctttgatttt gactgcatca cctggcttta ctgtaatcgt tttttcttca ggaattcccc
     6181 atttttgcca cgtgcgaacc acttcttttg gcccaatgaa tgttgcttca ggacaatttt
     6241 gatgaactgc tgcggctgta tggatatcta gatgatcgga atggatatgt gtaacgatta
     6301 aagcgtctac attttttatc gcaaaaggat cgattacgaa aggctgagta cgtaaattag
     6361 gctgcatatt ttgaacgcca ctcatacgca tcatctgatg gccttttttc atcatgccat
     6421 tcccatgact acgttttccc gtcccacacc atagatcaca taaaatattc gttccttcat
     6481 gacttttcag ccaaattccc gtacagccca gccaccacat agcaaaagtt ccaggatcca
     6541 ctacttctgt ttcgatttct tcattcaagt atgtgcccca ctcaggaaat gtttctacta
     6601 accacttttc ttttgttact tcattgacag ttttactcat ttgcttgctc cccccatatc
     6661 gttgttccaa cttacaagta aatattaatg tgtatcagca tccatgaaaa cccttttata
     6721 tgaccgtttg ttttttatat gattttcttt cagtaatcaa tgattgtttc agtataataa
     6781 agacagatat gaatgtttaa atgaacaact agtcaaataa atccatggat caagatgtcc
     6841 ctcaggaggc aaaagtcatg aaaaactcac ttgtcaatat tgaaaaacgt caaaaagaaa
     6901 ttttggaact gcttcagcag actgaacaac tatcaacttt cgaattagct gaatcccttg
     6961 atgtatctat tagtacgata cgaagagatt taaatttatt agaagaaaaa aacgagatca
     7021 tacgcaatta cggttattgt tcttataatt acaaaaacca gactgatttt gatcaatctg
     7081 gtcctgaaag gattaaacaa gcgattgcca gagaagctag taaatacatt aacgattatg
     7141 atacgctgtt tatcaattct agctcgactg cgttgaaaac actggatttt cttcaatcta
     7201 accatttgac agtcattaca aacaacgtga aaatcaatta ctctccgcac aaagataatt
     7261 gtagttatat tttaactggt ggagagatgc gctttccaaa agaagcacta gtgggcgaca
     7321 tcgcattaaa tacgatttct tcagtcaatg cagacatttg tatcattggc tgtagcggag
     7381 tcgatgtagt acatggggtc acaacaaaga tattaaatga atcaacgatc aacgaaatga
     7441 tggtcaacaa gacaatcaaa tgtaagattt tagtcgctga tcatcgaaaa atcggactga
     7501 catcaaagtt caagattgct gatatttcag tctttgatta tttgatcact gataaatttt
     7561 gttctgaaaa agtattagag gagatcagaa atacggggat aaaaaccatt caagtagatt
     7621 cattttaaga accaggacgt aaatatgtaa ataggattat caaaaaaatg aataacaaat
     7681 ttatcgataa atgaaaaaca cgctacgctc aaattgataa aggaatccca agatgtatcc
     7741 atccaatcga taccatcata atctattgct tcttttataa ccgttatatg ttgcctaaat
     7801 aggaatcaaa caatatcgaa aaaaggaggt atgacaaaag tcacacctcc ttttttcgaa
     7861 taaacggtat tcaaatcaga acactgattt cacaaccact ttatttcagg tcgttttcaa
     7921 tcacctttac cgcgtaatca tttccgttga aacgcgctat tttttttgcg tccatatatc
     7981 tttctaaata atcatcttct cctaattgct tatggcaaga agcaatttca taaagcaagt
     8041 attccaatgc ataagtcgat ttatgtttct tattatactc aatacccttc tcacataatt
     8101 ggattgcttt atcataatcc tcgattttac ggtaataggt ggcgatatca taatatacaa
     8161 tgcagaccat cagattatca aaatcagtct cactcaatat tttaagtgta cgctccacat
     8221 aaactttcgc aaaatctaat tctccttttg acaagtagat cattgccacg tttgcagctg
     8281 cttgtaagtg atggatatct gtttcggtcg tttgttccaa cacttgatta aaataaaata
     8341 atgctgcatc tggttttttc gcataaagat gatcaagcag tccttgataa aataaaattt
     8401 gatttaaaag atcagaatta cttacttttt cctgatcgat cgtttgtaaa attttataag
     8461 attcttggta ttttgataca gaattcaatg attgaatctc atttattttt ttcctgattt
     8521 tgtcttcttc tgtttctact aatatatcgg agatctcgac attcagcttt ttgcaaagcg
     8581 cacttaagat attgatgcct ggcgacatat tcttgttttc gatattgctg acagttgctt
     8641 gttcacaaat gccttctgct aattgtactt gtgttaaccc ctttttcttt cggtattctt
     8701 ttagtaatgc tccatctact ttaatcaaaa caatttcctt ccctttaaaa aattatttct
     8761 cctttatatc atacacaaaa aaaagttcta aacaaccaaa aatgtttaga acagaataaa
     8821 aattttaatt ttaaaataat attcaaatta aacaactggc gtatctgctc cttgatttct
     8881 ttttgtgtta tctgaataat tatcgtttac agttataaca catagtgaaa attgtcatgg
     8941 cgagaaagtc taagaatatt tttaaacata actatttctt tcttacgtgt gtataatgac
     9001 gacagcacac ctatcaatta atataattat tttatttttt tatgttctca tgtttatttt
     9061 taatgacaca acttctcgta tttatctggc acaacatata aatcgatcaa aaaattgttg
     9121 cgtgtttttt tgaaaattgg agtttcaact tgattacaaa atttaaattt agaagttgtg
     9181 aaaacagcga gagacagttt aatattcaaa atatacatag gctactcgaa cgctgtgttt
     9241 tttagtatac tagtaagaaa cgaagggata gactcattcc aataaggagg atttcatgaa
     9301 gacaattctt tttgctcctg cagtattcaa tttagctgag acaacacgaa tgattgaggt
     9361 tgccaaagct atgaaagacc gttattactg tgtattcttt ggcttttcta aaacttttgc
     9421 tcctttgatc acagaaagtg gctttgagta tcatttacta gcgccgatcc ttacgaaaaa
     9481 acaagaagaa caaatcataa aatttgacca gttgcgtaca cttaaaaacc cttttaccaa
     9541 agaaatggtt gcctctcgta tcactagtga acagagtctg attgctaagt ttgagcctcg
     9601 actgattgtg acaggttcga atgtgacaat ttttttatcg gcccgcaata aaaagattcc
     9661 tttagtatat gttaaaccct atgctttgag tgaacctttt tttcaatcag agcatgcgct
     9721 gatgccccac gagttacgtt tcttcggccc tttaagcaat cccctttggc actggacaaa
     9781 aaagcgtata gtaaataccc gatggatccc caatgccttc aaacacgctg cacaaaaaga
     9841 aatgcttcct ttaccaaaaa caagtttaca aatgatggat gctgatctaa atctcatcac
     9901 aacgcctaaa ctatttttgg aagaagaaca gctgccggaa aattatcgaa tcgttggtcc
     9961 aatttttgct aagctcagta cttccttgcc tgaagaagtg ctaactttta tacagcgaaa
    10021 gcaacaagaa agaaaacgta ttatctattt cgcaatgggt agttcaggca atccgcgtat
    10081 gttacgcaaa attttgttct tcttacaaaa acaaccggat ctagcggtta ttgcacctat
    10141 tgactatctg cttgatgaca ttcaatctga ctcagaagat cttttaataa cagaatatct
    10201 gtctagtcat cttttgcatg aattcattga tttttctttt atccacggcg gggaagggac
    10261 cgtccaaact gcctgtgaat ccggcaaacc gtttatcggt gtggggatgc aatttgagca
    10321 aagctgtaat attgactatt gtgtaaaata cggaaacgca atcgccctca caaaaccagt
    10381 gactgaaaga aaactgcaga ttgctctgcg cgaagtttct tcacccaaga ttcgtcagca
    10441 ggcacgcacg ctgcaagccg tttttccaat aaatggtcca caacaagcaa ttcaagaaat
    10501 cgaaaccttc ctgagcaacc acccaatata gtttcaagta gaaaaagctt ttttaataga
    10561 gactcatcag taatttcgct attcgagacc cagacagtga aaatacaaaa aaagtaaata
    10621 gtggattgaa aagttagagt tacttaaatt acggtgtcta aaaaacacaa aatcatcctt
    10681 aaaataaaag atctgacgct atccaaatga gcataggtag cgtctgccat ttattcttag
    10741 tcaaattctt tgacaccttt ctctggttgg atcatctcaa cgtttcttca aaagaaagac
    10801 cataataatt aggcaaatac attttcttaa aatgtgtcat atagatctag aagcatcgct
    10861 ttgtagagat ttgtgccgct agtcccctca acaattacta aatcaaatgt aggatttacc
    10921 actttatctt tgataatcaa cctccctctt ctgttgagac aatgatctcg caatccgatg
    10981 catactccta tataaactgc cagcaatccc cacatattga gtagatcatg tcagaatgtt
    11041 ctatgtcgtc agcaacgata cactaaaagt cacgttcacc atgactgatt gctattccca
    11101 gcacaattat ttctatacac gtttcatgat ttgaataaac actcacatca tacatattac
    11161 cattcataca ccatactgca gcccctacta tatggttgta agctcccaaa tctatatttg
    11221 acttgatcac tttggtagta gatctactaa ttctaaatcc attgctgata aaatttccat
    11281 tttttcctcc ttctttctgc caaaacagtt aaaacaataa taatctttat gtaaaaaagg
    11341 gctgtgacaa aagccttgtc acagtcccat attccgaata aacggtggtc aaaagcttgc
    11401 atctgcggta ataagttcaa ccaaacgaaa aataagccaa catttcacaa aaatttaaga
    11461 agcaattttc gtaaatttct tcttatttct tgaagctaac cacttctgtt acaaccactt
    11521 ttagaacatt taaagcttcg atgtgacaaa ccatctatgc tgctcgatca taaaattgat
    11581 agcaaaaatt ccacaaagta catatgtccc ccatgaaaac aaaacattca tatgcggtac
    11641 aatgatcttt ggaagaatca cgatcaaaaa tgatgccagt gcccaaatcc atcgattgat
    11701 ttgaatatga tatgtagcta gcatattcaa tataaggtga ctagccgtta ataaaaagat
    11761 aaaaacaaaa aataaaatca gccaatacat tgtttcccac cttctaaaaa ccaaacttag
    11821 acagtctgga cttcgttttc tccacgactt tgttcatctg acagcatctt tttatcataa
    11881 gcttttacaa atggataata gataataaat gctatcaatg tgttgatcat gatcaagata
    11941 acaacacgcc agtcatttcc tgctgaaaga taaccgccga ttggagctgg taatgtccat
    12001 gttggcaaaa cagtaaatct attgaccatt tctaatttga ctgcaaaata actgataata
    12061 gtcaaaacta aaggaactaa gttgaacggg atgaacatgt aaggattcaa catcatagga
    12121 gctccaaaaa ttatgggctc attgatattg aagataccag gaataataga aaaacgacca
    12181 acctctttca agaattttga tttacagaag aatataaaca aaacagatac cacgatcgtt
    12241 gctcctgcac caccaattgt taccgtccat tgataaaact gttcaggagc gatatagggt
    12301 aattgggtgg cagctgtacc atctgccaat gcctttgcat tctcatccaa catgatcagc
    12361 cacattggac gaacaacagc ccctacaata ctcatgccat ggataccaaa tgaccacaac
    12421 aaatggatca aaaacattgg tagtaaaaca ccaagcaaat tattccctaa aatattggtt
    12481 aaaggctcga ataacgataa taaggcagcg tttacatcaa agcccgcgac gcttcgaatc
    12541 agccaagtaa ttgttaaaat gactgcacct ggaatcaatg cctcaaaact acgggcaaca
    12601 gcaggtggca cttgttctgg cattttgata gtaatatttc gatttttaaa gaaacgatat
    12661 acttccactg caaaaatcat agataagatc cccgcaaaca tcccagatgc tcccaaagaa
    12721 ctcataggta agacccagcc aagaggtgcc tcttcaggag ttaaaacgtt tacaggaact
    12781 tgtaacataa agaacgttcc taaagataat ataccacctg tcacactatc taatccatat
    12841 gatttagcta gatccgaacc cataacaaaa gttgcataaa tagccatcaa tcccatcgtc
    12901 atcctaaagg gtaaaacaat cgttgccgaa taaggggcaa taaattcatt ccatccagga
    12961 aacggtaaat tgataaagac cataaaaaat gtcccaatca aaatcaatga caatgttgaa
    13021 acaaccccat tacgaattgc taataaatga cgctgtgtac caatttttgc cattggtggg
    13081 acaagtttct cttcgaccca tttcatcagc ttttccacta aaaccactcc ttttcaataa
    13141 aatatgaacc attc
//
LOCUS       DACDKM010000078        13113 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20834_2016
            SAMN12640370-rid9891663.denovo.078, whole genome shotgun sequence.
ACCESSION   DACDKM010000078 DACDKM010000000
VERSION     DACDKM010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640370
            Sequence Read Archive: SRR10040891
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13113)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13113)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742835.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742835.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:04:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,012
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 29 of 75
            Pseudo Genes (incomplete)         :: 33 of 75
            Pseudo Genes (internal stop)      :: 19 of 75
            Pseudo Genes (multiple problems)  :: 5 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13113
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640370-rid9891663.denovo.078"
                     /isolate="E20834_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            2..1741
                     /gene="yfcC"
                     /locus_tag="IV334_002008"
     CDS             2..1741
                     /gene="yfcC"
                     /locus_tag="IV334_002008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative basic amino acid antiporter YfcC"
                     /protein_id="HAP8944067.1"
                     /translation="MKKTNSSGKPKRDFTKLSTPHTYVIIFGVVIFAWILTFVVPAGK
                     FSTQDIEYKDANGETSTRTVLRQDSFRYAYELDKSYVFDQLEELQDHPAEREKLDVPE
                     KGLEKVIADGEKNLTQEKLDEISLTDDVLYDQYGENIYDTSKKLHKTAKIWGTDDFGG
                     FGFLNFVFEGLVSGDKYGSAVGIAALILVVGGAFGIIMRTGAIDAGIYAFISKTKGLE
                     RLALPLLFFAFSFGGATFGMAEEVIPFSMVMVPFVIALGYDSIVAVTVTYVASQVGNA
                     TSWMSPFSVAVAQGIAGIPVLSGATFRLIMWVVVTALAAGYMMIYAEKIRKKPESSLT
                     YKSDDYFRSHIKKTSDENKKFMLGHKLILLEMLVVLVWIVWGVTQKGYYIPEIASQFF
                     VMGLVAGIIAVLFKLDGMKVNDIASSFQSGAADLAGTAIVVGMAKGILLVLGGSDASV
                     PSALNTILHGIGTALTGVPAVIGAWAMYIFQSLFNLVVTSNSGQAALTMPIMAPLADL
                     VGVSRQVAVLAYQLGSGFMDAFTPVSASLIGVLGVARIEWAKWARFQIKMQGFLFVLG
                     TIFIMIAIAIGLQ"
     gene            1898..3082
                     /gene="iadA"
                     /locus_tag="IV334_002009"
     CDS             1898..3082
                     /gene="iadA"
                     /locus_tag="IV334_002009"
                     /EC_number="3.4.19.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
                     /protein_id="HAP8944068.1"
                     /translation="MKIIRQIEVFAPDYLGKMDVLIAGGKILAVEEQLEGGYEGVEVE
                     ELSGEGKVLTPGFIDCHFHILGGGGEGGYQNRTPEVTLSQLTTAGVTTVVGCLGTDGE
                     GRDMTALISKAKGLEAEGISTYVYEGSYRLPVKTVTDSIIKDFLTIDKIIGIGEIAVS
                     DHRSSQPSFEEFARAVADARVGGMLSGKAGIINVHLGGGKRKLELLTRIVEETEIPIT
                     QFLPTHANRTPELFEACVAFAKRGGTIDFTASEDPDFWEKTDGEVRFSKALKRLIEED
                     VSLDNFTMTSDGQGSLPYFDENNHFLGLGVGSAKALLVGIKEAVQKESIPLEIALRAI
                     TSNPARILKLDKKGKIEIGADADLCILDKETLDIDTVIAKGEIMVQEKEVKVWGTFEK
                     SF"
     gene            3193..3396
                     /locus_tag="IV334_002010"
     CDS             3193..3396
                     /locus_tag="IV334_002010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HAP8944069.1"
                     /translation="MADLKGRSKDMKDKVVGEGKEAYGKATDDKSKETEGKAQSLGAD
                     IKEKARNLGDEVKDKFSRKDEKK"
     gene            complement(3497..4159)
                     /locus_tag="IV334_002011"
     CDS             complement(3497..4159)
                     /locus_tag="IV334_002011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP8944070.1"
                     /translation="MLRTEQLGFWYQDPQDALYKDVELNFEAGKMYAILGASGSGKTT
                     FLSLISGLDAPKEGKVYYQDRSLNKIGLRSYRKNDVSIIFQAYNLLPYLSALDNVIAA
                     MEITKTKQENYRQVALDRLARVGIDEILAKKQVTKLSGGQQQRVAIVRAICCEHQLIV
                     ADEPTGNLDETTSQDIVKLFQEIAHEQNRCIIIVTHEIEVAKSCDEVFELKNKKFQKI
                     EV"
     gene            complement(4172..5674)
                     /locus_tag="IV334_002012"
     CDS             complement(4172..5674)
                     /locus_tag="IV334_002012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP8944071.1"
                     /translation="MNFMKRAWKSTKVKWGRSLLLFAVFTAILIFVLAGLTIRSAAEE
                     AANQAQKEVGATVTLSANREASFKKQEQTSSSGSTTEERPDPGSFSLTPVSLTDAEKI
                     AELDNVKSYSFESSASAMAKDGITAITSSDSSEDSSETTDAGGEPAGGMGGNPQMTQA
                     DFQISGVSATAQNSNFSDGTAKITDGEGIDDSDKGTNNVVIDSTLAETNDLAVGDTFT
                     ITSTEDEDTTYEMTIKGIYESSETSGSMGMKFNFMNPSNTLYTSYTFANELNGTSDDN
                     TIDSAIYTLSDPDKMDEFLKEAEKLIDTDTFSLQSNDSMYQSMLEPLNNVASFSKNVV
                     LLVAVAGIIILTLIIMITIRERRHELGVLLSLGESRSKIILQLFTEVAICMILALGVA
                     SLSGNVVANAVGQQLLDQQTETTTQNQQAPGGGQMPGNRQEQGGQRDGQQGTTNNPFA
                     VSDQVSELEINVKPAQLGLLAGVAFGISLFSVFLASIGILRLNPRKILLN"
     gene            6222..7901
                     /locus_tag="IV334_002013"
     CDS             6222..7901
                     /locus_tag="IV334_002013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HAP8944072.1"
                     /translation="MSTIKIIPLGGVRENGKNMYVAEVGEDIFILDCGLKYPENELLG
                     IDVVIPDFTYLEENIDRVAGVFLTHGHADAIGALPYLLSKISVPVFGTELTIELAKLN
                     VSRNEAAKGFKDFHVVDEHTEIDFGETVVSFFRTTHTIPDSVGINLKTKEGSIVYTGD
                     FKFDQTAIPMYQTDYARLAEIGKEGVLALLSESANAENPQPVASEAQIADEVFDTIKY
                     WEGRIIVACVASNLQRVQQVLDAAEKAQRKVVLTGQDFERIIRTAMRLEKLQLPSEDL
                     LVTLKDMKNYDPEELIILETGRMGEPIKSLQKMANGTHRNVKIEEGDLVYITTTPSIA
                     METIVAKTEDIIYRAGGTVKLISENMRVSGHANPNDLQLMINLMKPTYFVPVQGEYRE
                     LAAHADLAHAVGMPYKNIYITGRGDVLEYKNKRMSVAGTTSAENVMIDGLGIGDIGNI
                     VLRDRKILSEDGIFVAVVTISRREKKIISKPQITSRGFVYVKASRDLMKESSNMIETI
                     VEKHLESNDFEWSKLKQDIRDQLGRYLFEQTKRRPVILPVIMEATQRRNKK"
     gene            8028..8507
                     /locus_tag="IV334_002014"
     CDS             8028..8507
                     /locus_tag="IV334_002014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA deacylase"
                     /protein_id="HAP8944073.1"
                     /translation="MMSQTKVERYLEEQKISYQPLDFSHVVEESFTEELQKRGIDPAL
                     ICKTLVVKGNKTGVIIALIPLNDHLDYKKTRQVTKDRKVGFPGMDFVLMHTGYPHGAN
                     TPIGIKLAHPDYCFLADSSLKEKEEVIVSSGEIGRSLRIKVADLEKIVHPIYADLIS"
     gene            8726..9406
                     /locus_tag="IV334_002015"
     CDS             8726..9406
                     /locus_tag="IV334_002015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HAP8944074.1"
                     /translation="MKITYHGHSCLTIEMENGQRVMIDPFITGNPLTDLTAEEVKTDW
                     LLITHGHSDHIGDMIPIAKENQATIISIVEIADYANSRGVDSFGMNIGGKHAFPFGVV
                     KFVHAQHSSSYEVDGIVQYMGEPSGIIIQAEGKTIYHAGDTAYFSDLGLLAEEFDIDV
                     AFLPIGDNYTMGPEDAAKAAQTIRAKKVVPIHYNTFPVIEQDPESFVELLEDSEGKIM
                     SVSETLEV"
     gene            9414..10745
                     /locus_tag="IV334_002016"
     CDS             9414..10745
                     /locus_tag="IV334_002016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HAP8944075.1"
                     /translation="MATKHDLILEYIESLPVGNRISVRSIAKELNVSEGTAYRAIKDA
                     ENVGLVSTIQRVGTIRIERKLKKHIERLTFGEVVRIIEGDVLGGAAGLDKVLNKFVIG
                     AMTKHAMERYITPGSLMIVGNRAEVQKLALEDGAAVLITGGFNTTAEIAKLADDLEMP
                     ILRTSYDTFTVATMINRALSDQLIKKDIMLVSDIYMPAEKTHYLQQTDTISDYQRLSE
                     ETQHSRFPVVNRHHRLMGIVTAKDVLGKSPNQIIDRVMTKEPISVKKTMSIASVSHQM
                     IWDGLEVMPVVSDDLTLEGLITRQDVMKAMQLVQRQPQIADTISDQISGNIHTIDTDR
                     EGNRLENPKFKFVVAPQMVNGVGTISFGVLSEIISDVAQKTMVMNQKRNILIEQVNLH
                     YLRLIQLESELDIRPRVLEIGRRSAKLDIEVFIENTIVSKAIVVCQVMERS"
     gene            10760..11722
                     /locus_tag="IV334_002017"
     CDS             10760..11722
                     /locus_tag="IV334_002017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional oligoribonuclease/PAP phosphatase
                     NrnA"
                     /protein_id="HAP8944076.1"
                     /translation="MTVQQEIVDTIKKYDKIIIHRHLRPDPDALGSQVGLAELLRESF
                     PEKEIRQVGETIEGLRFLAEMQEVEDVFYQGALVIVTDTANSPRISDQRYQLAEKLIK
                     IDHHPNDDPYGDLVWVNTKASSCSEMIAEFALMFPESLKMNVKAARLLYAGIVGDTGR
                     FLYPSTTSRTLEIASILRNYPFDASALNREIEQMPMKVAKLSGYLYQNIQVDENGAGR
                     IILPQEILDKYGIDDSETASIVSLPGVIDEVLAWGIFVQQPSGYYRVRLRSKGPIINE
                     LAKRHHGGGHPLASGANAKDLAEVEEIYKEIQDICQSYKSRKTE"
     gene            11833..12171
                     /locus_tag="IV334_002018"
     CDS             11833..12171
                     /locus_tag="IV334_002018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkylphosphonate utilization protein"
                     /protein_id="HAP8944077.1"
                     /translation="MTLPNCPECGSEYTYEDRGLLICPECGNEWNKEEQETVIEDGLV
                     VLDANGNRLLEGDSVTVIKDLKVKGASGAIKQGTKVKNIRLVEGDHNIDCKVEGFGPM
                     KLKSEFVKKN"
     gene            12250..12957
                     /locus_tag="IV334_002019"
     CDS             12250..12957
                     /locus_tag="IV334_002019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metallopeptidase"
                     /protein_id="HAP8944078.1"
                     /translation="MYGYGMYGFLDPTFLLVILGLVISGIASAYVNSTFRKYDQVRSK
                     NNVTGTQAAQYILQSQQINNVGVQQIAGDLTDNYNSGNKMLSLSQATAQSTSVAAIGV
                     AAHECGHAVQDASGYVPLKIRAALVPAANFGSMISFPLIMVGVLLSWNSTLINIGIFA
                     FSLALLFQLVTLPVEFNASRRAIQILSEGGLLTEEEVPMARHVLFAAALTYVAAALST
                     FLQLLRLILLFGGNRRD"
BASE COUNT         4153 a         2325 c         2890 g         3745 t
ORIGIN      
        1 tttgaagaaa acaaattctt caggaaaacc gaaacgcgac ttcactaaat tatcgacacc
       61 ccatacgtat gtcattattt ttggtgtagt catctttgca tggatactga cttttgtcgt
      121 tccagcagga aagttcagca cgcaagatat tgaatacaaa gatgcaaatg gcgaaacaag
      181 tactcgtact gttttacgtc aagattcctt ccgttatgct tacgagctgg ataagtcgta
      241 tgtgtttgat caattagaag aactgcaaga tcatccagca gaacgtgaaa aactggatgt
      301 tccggaaaaa ggattggaaa aagtcattgc tgacggagaa aaaaatctta cgcaggaaaa
      361 actagatgaa atctccttga cagatgatgt gctttacgat cagtacggcg aaaatatcta
      421 tgatacttct aaaaaattgc ataaaacagc caaaatatgg ggaacagatg attttggcgg
      481 ctttggtttc ttaaattttg tttttgaagg actagtatct ggagataagt atggatctgc
      541 tgtagggatc gctgcattga tccttgttgt tggaggtgct tttggtatta ttatgcgtac
      601 cggagcgatc gacgcaggta tctatgcgtt tatcagtaaa actaagggac tggagcgttt
      661 agctttaccg ctattattct ttgctttttc attcggtggc gctacattcg ggatggcaga
      721 agaggtaatt ccattctcaa tggtgatggt tccatttgtc attgcgttag gatatgactc
      781 gatcgtagca gtaaccgtga cttatgtcgc atctcaagtc ggaaatgcga cctcttggat
      841 gagcccgttt agcgtagcgg tggctcaagg gattgctgga atccccgtgt tgtctggtgc
      901 gacattccga ttgattatgt gggtagttgt tactgcatta gccgctggtt atatgatgat
      961 atatgcagaa aaaatccgta aaaaaccaga aagttcgttg acttacaaat cagatgatta
     1021 tttccgttct catatcaaaa agacatctga tgaaaataaa aaattcatgc ttgggcataa
     1081 attgatctta ttagagatgt tagttgtcct tgtctggatc gtttggggcg tgactcaaaa
     1141 aggctattac attccagaaa tcgcttctca attctttgtg atgggattag ttgctggaat
     1201 cattgctgtt cttttcaaat tagatggaat gaaagtcaat gacatcgctt cttctttcca
     1261 atctggtgca gctgatttag ctggtacagc aatcgtagta ggtatggcca aagggattct
     1321 tcttgtcttg ggaggatctg atgcatctgt tccttcagct ttgaatacga ttttgcatgg
     1381 gatcgggacg gcgttgaccg gtgttccagc tgtcattggt gcttgggcga tgtacatctt
     1441 ccaaagtttg ttcaacttgg ttgttacatc aaattcagga caagcagctt tgactatgcc
     1501 aatcatggct ccactggcag atttagtagg tgtatccaga caagtagcag ttcttgctta
     1561 tcagcttggt tcaggtttta tggacgcttt tacacccgta tcagccagct tgattggtgt
     1621 gctaggtgtt gcacggatcg aatgggcaaa atgggcaaga ttccaaatca agatgcaagg
     1681 attcctgttt gttttaggaa ctattttcat catgattgcc attgcaatag gtctgcagta
     1741 gtactggaga cctgtaaata aaaaagtaat ccaaacagta gataaggact ccaagaaccg
     1801 tcattttacg gtaaaatcaa aggagtaaag atcctatcgt ttggattatt ttttaagatc
     1861 ctatcatcag ttatacttaa agaaagagga aaaatgtatg aaaatcattc gacaaattga
     1921 agtgtttgca ccagattact taggaaaaat ggatgtactg attgccggtg ggaagatttt
     1981 ggctgtagaa gaacagctag aaggcggcta cgaaggagtg gaagtagaag aactttcagg
     2041 tgagggaaaa gtactaaccc cgggtttcat tgactgtcat ttccatattt taggtggagg
     2101 aggagaaggc ggttatcaaa atcgaacgcc agaagtcaca ttgagccaat tgactacagc
     2161 tggtgtaacg acagttgtcg gttgtttggg aacagacgga gaaggtagag atatgactgc
     2221 gttgatcagc aaagcgaaag gtctggaagc agaaggaatc tccacttatg tatacgaagg
     2281 ttcgtaccga ttgccggtaa aaacagtaac agattctatc atcaaagact ttttgacaat
     2341 tgataaaatt atcggaatcg gagaaatcgc agtatccgat caccgttctt ctcagccaag
     2401 ttttgaagag ttcgctcgtg cagtagcaga tgctcgtgta ggaggcatgt tatccggaaa
     2461 agctgggatc atcaatgtcc acttaggcgg aggaaaaaga aagctagagc tattgacacg
     2521 aatcgtagaa gaaacagaaa ttccgatcac acaatttttg cctactcatg caaatcgcac
     2581 accagaatta tttgaagctt gcgtggcatt tgccaaacgt ggtggcacga ttgacttcac
     2641 tgctagcgaa gatcctgatt tttgggagaa gacagatggt gaagttcgct tcagcaaagc
     2701 actgaaacga ttgatcgagg aagatgtttc tttagataat ttcacgatga cctcagatgg
     2761 acaaggaagc ttgccttatt ttgatgaaaa caatcatttc ctagggttag gagtaggaag
     2821 tgccaaggca ttgctagtag ggatcaaaga agctgtgcaa aaagaaagta tccctttgga
     2881 aatcgcctta cgagctataa ccagtaatcc agcacgtata ttgaaattgg ataaaaaagg
     2941 aaaaatcgaa attggtgctg atgcagatct gtgtatcttg gataaagaaa cgcttgatat
     3001 tgatacagtt atcgctaaag gagaaataat ggtccaagaa aaagaagtca aagtatgggg
     3061 gactttcgaa aaaagttttt aatcaacagc tctaactatc ttttttagaa aacgagcgtt
     3121 ctgtttgtct gtatctgttg ttgatgttac cctgaaggta gatcaataaa caaactaaga
     3181 ggaggaagca caatggcaga tttaaaagga cgttctaaag atatgaaaga taaagtagtc
     3241 ggtgaaggaa aagaagcata cggcaaagca acagacgaca agagcaaaga aacagaaggg
     3301 aaagcacaat cactaggtgc tgacatcaaa gaaaaagctc gaaatttagg cgacgaagtc
     3361 aaagacaagt tttctcgaaa agacgaaaaa aaataaaaac cacttattac taattgaatc
     3421 aagcaaataa aaaatccgaa ccatatctgc gagacagata tggttcggat tttttatttg
     3481 atcattatcc tgttttttaa acttcgattt tttgaaattt tttatttttt agttcaaaga
     3541 cttcatcaca gctttttgct acttcaatct catgagtcac gatgataata cagcgatttt
     3601 gttcatgggc gatttcttgg aaaagtttga cgatatcttg tgaagttgtt tcatctagat
     3661 ttccagtagg ctcatccgct acgattagtt gatgttcaca gcagatagca cgaacgatag
     3721 cgactcgctg ctgctgacct cccgaaagtt tggtcacctg tttttttgct aatatttcat
     3781 caattcctac acgagccagt cgatctaaag caacttgtcg ataattctct tgcttggtct
     3841 tagtgatttc cattgctgct atgacgttgt ctagagccga taaataagga agcaagttgt
     3901 aagcttggaa aataatcgag acatcatttt tacgataact tcttaacccg attttgttca
     3961 gtgaacgatc ttgatagtat acctttcctt cttttggtgc atctagtcca gaaataagcg
     4021 aaagaaaagt cgtttttcca gaaccacttg ctcctaaaat agcgtacatt tttccagctt
     4081 cgaaattcag ttctacatct ttataaagcg catcttgcgg atcttgatac caaaatccca
     4141 attgttctgt ccgtaacatc aaattcctcc tttagtttaa taggattttt cttgggttta
     4201 atcttaatat ccctatagaa gctaaaaaga cagaaaacag actgattcca aatgctactc
     4261 cggctaatag tcccaattgt gcaggtttga cattgatttc caattcgctg acttgatcag
     4321 aaacagcaaa tggattattc gtggttcctt gttgaccatc gcgttgaccg ccttgttctt
     4381 gtctgttacc tggcatttgt ccaccgccag gagcttgctg gttttgtgtc gtggtttctg
     4441 tctgttggtc aagtagctgc tgtcccacgg cattcgcgac gacgtttccg ctaagcgaag
     4501 caactcctaa tgcaagaatc atacaaatag ccacttcggt aaacaattgg agaatgattt
     4561 tacttctgga ttctcctaaa gatagcaaga cgcctaactc atgccgacgc tcccgaatgg
     4621 taatcataat gatcagagta agtatgataa taccggctac agccactagt aaaacaacat
     4681 ttttagaaaa acttgccacg ttgtttaaag gttccaacat tgactgatac atggaatcat
     4741 ttgattgtaa gctaaatgta tctgtgtcga tcagcttctc agcttctttg agaaactcat
     4801 ccattttatc tggatcagat agagtgtaaa tagcagaatc tatcgtgtta tcgtcagatg
     4861 tcccatttag ttcatttgca aaagtgtaag aagtgtaaag tgtattcgac ggattcatga
     4921 agttgaattt catacccata ctgccgcttg tttcgctgga ttcataaatt cctttgatcg
     4981 tcatttcata agtggtatct tcatcttcag tactcgtgat tgtgaaagta tctccgactg
     5041 ccaggtcatt tgtttctgct agagtagaat caatgactac attgtttgtg cctttgtcgc
     5101 tgtcatctat tccttcacca tccgtgattt ttgctgttcc atcagaaaaa ttactatttt
     5161 gtgcagttgc tgacacacca ctgatttgaa agtctgcttg agtcatttgc ggattcccgc
     5221 ccattcctcc agcaggttca ccgcctgcat cagtagtttc tgaactgtct tcacttgaat
     5281 cagaactagt gatggcagtt attccatctt ttgccatagc tgaggcggaa ctttcaaagg
     5341 aataactctt tacattatct aattcagcta ttttttccgc gtccgttaag gaaactggtg
     5401 taagagaaaa actgcctgga tctggacgtt cttcagtggt agaaccgctt gaagaagttt
     5461 gttcctgttt tttaaatgaa gcttctcgat tggcacttaa agtcactgtt gctccaactt
     5521 ctttttgtgc ttgattcgct gcttcttctg cagcgcttcg aatcgtgagg ccagctagca
     5581 caaaaataag aatggctgtg aacacggcaa acaataacaa tgaacgtccc cattttactt
     5641 ttgtactttt ccatgcacgt ttcataaaat tcatattttg catccctcct agtagatgca
     5701 tttgataata gacaacctat cttaaagcta gcttaaatct tatgctgttt gcacaccatc
     5761 aacatgaggt tctgtatata taaaaagtca ctatagttat tgcaaaaaat gtggtaaaat
     5821 gaaaaaagta tgtttcaaac cgacgaagaa gaaatgaaag aggaatagca aaagaaatcc
     5881 tagatgctac agagggattt ttacgtgcat agttgttcta acaattgctg atactagcga
     5941 tttagtttat ttgttgtata ataatgggga acagctgaat acttatataa gtattctggc
     6001 tatagcgttt cgcaaaagat ccattttgtt aatgaagtat atccaaaata aaatgcccat
     6061 ccaactgtca gaacgcggat gtgctggtaa ttttcctgct gattctccat aagttgagaa
     6121 atagtgattt tgcatatgct tcattggaat ggctcatata tttagataaa gaagaaaatc
     6181 gacatcttta aacttggatg tcataagaag aggtgaagtt tgtgagtacc attaaaatta
     6241 tcccattagg cggcgttcgt gaaaatggaa aaaacatgta tgtcgcagaa gtgggcgagg
     6301 atatttttat tttagattgt gggctaaaat atcctgaaaa tgagttgttg gggatcgacg
     6361 tcgtaatccc tgattttacg tatttagaag aaaacattga tcgtgtagct ggtgtctttt
     6421 taacacatgg acatgcagat gcgatcgggg cacttcctta cttgttgtca aaaatttctg
     6481 taccagtatt cgggacagag ttgacgatcg agttagcaaa attgaatgtc agtcgtaatg
     6541 aagcagccaa aggatttaag gatttccatg tggtagatga gcatacagaa atagactttg
     6601 gtgaaacagt agtgagcttt ttccgtacca ctcatacgat accagattca gtggggatca
     6661 atctgaaaac aaaagaaggt agtatcgttt atacgggaga ttttaaattt gaccaaacgg
     6721 ctatcccaat gtatcaaaca gattatgcac gattggcgga aatcggcaaa gaaggagtct
     6781 tagccttact aagtgaatcg gcaaatgcag aaaatcctca gcctgtagct tctgaagccc
     6841 aaatagcaga tgaagtattt gatacgatca aatattggga agggcgtatc atcgtcgctt
     6901 gtgtagcaag taatttgcaa cgagtgcaac aagtattgga tgcagcagaa aaagcacaac
     6961 gaaaagttgt cttgactggt caagatttcg aacgaattat tcgcacagcg atgcgtttag
     7021 aaaaacttca attaccaagt gaagatctgc ttgttacgtt gaaagacatg aaaaactatg
     7081 atccagaaga actgatcatt ttagaaactg gccgaatggg tgaacccatc aagtctctgc
     7141 aaaaaatggc aaacggtaca caccgtaatg tcaagattga agaaggagat ttggtttata
     7201 tcactactac gccaagcatc gcgatggaaa ccatcgtggc gaaaacggaa gatatcattt
     7261 atcgagctgg tggaacagtg aagttgatct ctgagaatat gcgcgtttct ggccatgcca
     7321 atcctaatga cttgcaatta atgatcaatt tgatgaaacc aacatatttt gtgccagttc
     7381 aaggagagta tcgtgaatta gctgctcatg cagatttggc tcatgcagta gggatgccat
     7441 acaaaaatat ctatattact ggacgcggag acgtgctgga atataagaac aagcgtatga
     7501 gtgttgcagg aactacaagt gcagaaaatg taatgattga tggattaggc ataggtgata
     7561 tcggaaacat tgttttacgt gatcgaaaaa tcttgtcaga agacgggatt ttcgtagcgg
     7621 ttgtcactat cagtcggcga gaaaagaaaa tcatttcaaa acctcaaatt acttcacggg
     7681 gattcgttta cgtaaaagct agccgtgatc tgatgaaaga aagcagcaat atgattgaaa
     7741 caatcgttga gaaacattta gaaagcaatg attttgagtg gagcaaatta aagcaagaca
     7801 tccgagatca attaggccgt tacttatttg aacaaacaaa acgccgccca gtgatcttgc
     7861 cagtcattat ggaagctact caacgtcgaa ataaaaaata aacaaaaaga gtaagatgaa
     7921 cctctctata gagtgctcgt cttgctcttt ttttgcgaaa aacaaataat ttttgaatac
     7981 agcatccctc gctttataat aaacgataga ataaagggag tgaatcaatg atgagtcaaa
     8041 caaaagtcga acgttattta gaagaacaaa agatcagcta tcaaccactt gattttagtc
     8101 atgtggtaga agaatctttc acagaagaac tacaaaaaag agggattgat ccagctttga
     8161 tatgtaaaac tctcgtagtg aaaggaaaca aaactggggt gatcatcgca ttgattcctt
     8221 tgaacgatca cttagattac aaaaaaacga gacaagtgac gaaagatcgt aaagttggtt
     8281 ttcctggaat ggattttgta ttaatgcata caggatatcc ccacggagca aacaccccta
     8341 ttggaataaa actcgcacat cctgattatt gttttttagc agacagttct ttaaaagaaa
     8401 aagaagaagt aattgtgtca agcggtgaaa tcgggagaag ccttcggata aaagtagccg
     8461 atttagaaaa gatcgtccat cctatttacg ctgatttaat tagttaaggg acgagaagag
     8521 aagctaagca gagcagagac ctcaaacttg agattatatc tgcttggtac ttggtgcatc
     8581 ctgtattatc tgttacaatt gctttgtaca tactgtacta tcaatccaat gaaaatatat
     8641 ttagcagcta tattttttca ttgatgtgca agtgatgaaa tcgtttttaa taaaaaagat
     8701 acagagaaaa cgcgaggtgt aaaatatgaa aattacgtat catggacatt cctgtttaac
     8761 aattgaaatg gaaaatggcc aacgcgtgat gattgacccg tttattacag gaaatccatt
     8821 gacagactta actgcagaag aagtaaagac agattggttg ctgattactc atggtcatag
     8881 tgaccacata ggagatatga ttcctattgc aaaagaaaat caagccacaa ttatcagtat
     8941 tgttgagatt gctgattatg cgaacagcag aggagtagat tcattcggta tgaatatcgg
     9001 tggaaaacat gcgtttccat tcggcgtggt aaaattcgta catgcacagc atagttcaag
     9061 ctatgaagta gacggtatcg ttcagtatat gggcgagcct agcggaatta ttattcaagc
     9121 agaaggaaaa acgatttatc atgcagggga tactgcttat ttcagtgatc taggattgct
     9181 tgcagaagaa ttcgatattg atgtcgcttt cttgccgatt ggcgataact atacgatggg
     9241 gccagaagat gcagcaaagg ctgcgcaaac aattcgtgcc aagaaagtag ttccaatcca
     9301 ttacaatact ttccctgtca ttgaacaaga tcccgaatct tttgtagagt tactggaaga
     9361 tagtgaagga aaaataatga gcgtctcgga aacgctggaa gtgtaggcag aaaatggcga
     9421 caaaacatga tttgatttta gaatatattg aaagtttacc tgttggcaat cgaatttctg
     9481 taagaagcat cgcaaaagaa ttgaatgtca gtgaaggaac agcgtaccgt gcaatcaaag
     9541 atgcagaaaa tgtcggacta gtttcaacaa ttcaacgagt agggaccatc cgtatcgaac
     9601 gcaaattaaa aaaacatatt gaacgtttga catttggcga ggtcgtcagg atcattgaag
     9661 gggacgtttt aggcggggcg gcaggattag acaaggtctt gaataaattc gtcatcggcg
     9721 cgatgactaa acatgccatg gaacgctata ttacgccagg atcactaatg atcgtcggca
     9781 accgggcgga agtccaaaaa ttagcattgg aagatggcgc cgcagtattg atcacaggag
     9841 gattcaatac gactgctgag attgcaaagc tagctgatga tcttgaaatg ccgatcttac
     9901 gcacctctta tgatacattt acagtcgcaa ccatgatcaa ccgagcgtta agcgaccaat
     9961 tgatcaaaaa agatatcatg cttgtcagtg atatctatat gccagctgaa aagactcatt
    10021 atttgcagca gacagatacg atttctgatt atcagcgttt gtcagaagaa acgcagcatt
    10081 cgcgttttcc agtcgtcaac cgccatcatc gtttgatggg gattgttacg gcaaaagatg
    10141 ttttagggaa atcgccaaac cagatcatcg atcgggtgat gacgaaagaa ccaatcagtg
    10201 taaagaaaac aatgagtatt gcctctgtta gccatcaaat gatctgggat gggctagaag
    10261 tcatgccggt cgtttccgat gacttgacat tggaaggctt gattacccgg caagatgtaa
    10321 tgaaagcgat gcagcttgtc cagcgacaac cgcagattgc agatacgatt tcggatcaga
    10381 tatctggaaa tatccatacg attgatactg accgtgaagg aaatcgtttg gaaaacccta
    10441 agttcaaatt cgtagtagca ccacaaatgg tcaatggcgt tggaaccatt tcttttggcg
    10501 tacttagcga aatcatttct gatgtcgcac aaaaaacaat ggtgatgaac caaaaacgaa
    10561 atatcttgat tgaacaagtg aacctacatt atttgcggct gatccaattg gaaagcgaat
    10621 tagacattcg tccacgtgta ttggaaatcg gcagacgttc cgctaaactg gatatcgaag
    10681 tgtttattga aaacaccatc gtttccaaag caatcgttgt ttgtcaagta atggaacgtt
    10741 cttagaaagc aggagtgaaa tgacagtaca gcaagaaata gtagatacaa taaaaaaata
    10801 cgataaaatc attatccaca ggcatttgcg ccctgatcct gatgcattag gatcacaagt
    10861 aggattagcc gaactattac gggaaagttt cccagaaaaa gaaatacgtc aagtaggtga
    10921 aacgattgaa gggcttcgct ttttagcaga aatgcaagaa gttgaagatg tcttctacca
    10981 aggagcgcta gtcatcgtga ctgatacagc taattcaccc agaatcagtg atcaaagata
    11041 tcagttggca gaaaaactga tcaaaatcga ccatcatccc aatgatgacc cttatggaga
    11101 tcttgtgtgg gtaaatacaa aagcaagtag ctgtagtgag atgattgcag aatttgcttt
    11161 gatgtttcct gaatctttaa agatgaacgt taaggctgca cggttacttt atgcaggaat
    11221 cgtcggagat acagggcgct ttttgtatcc ctccacgacg tcccgtacat tagagattgc
    11281 ttcgatattg agaaattatc catttgatgc aagtgctttg aacagagaga ttgaacaaat
    11341 gccgatgaaa gtcgctaaac tttccggtta tctttatcaa aatatccagg tggatgaaaa
    11401 tggtgcagga agaataatcc ttccgcaaga aattcttgat aaatatggaa tcgatgattc
    11461 agagacagct tcgatcgttt cacttcctgg agtgattgac gaagtgctgg cttgggggat
    11521 ttttgttcag cagccttcag ggtattaccg cgtacgttta cgttctaagg ggccgatcat
    11581 caatgaattg gctaaacgcc atcatggagg aggccatcca ttagcaagtg gtgcgaatgc
    11641 aaaagattta gcagaagtag aggaaatcta taaagagatc caagatatat gccaatcgta
    11701 taagagcaga aaaacagaat gatacttcgt tcgaaaacgt gccttttgta gtaaaaatca
    11761 caaatgtatc ttttcaagat cttctttttc cgttacaata gtgctcacta aagattttaa
    11821 aggagaaaga agatgacatt accaaattgc ccagaatgtg gctcagagta cacatatgaa
    11881 gatcgaggct tgttgatttg cccagaatgc gggaacgaat ggaacaaaga agaacaagaa
    11941 actgtcatag aagacggatt agtggtatta gatgcaaatg gaaaccgtct gttagagggc
    12001 gatagcgtga cagtgattaa agacttgaaa gtaaaaggtg cgtcaggtgc cattaaacaa
    12061 ggaacaaaag tcaagaacat ccgtttagta gaaggcgatc ataatatcga ttgcaaggta
    12121 gaaggatttg gaccgatgaa actgaaatcg gaatttgtga aaaagaatta aagaatattc
    12181 aaggtctttt cacattcttt tcctactact aatgataaaa taaagtaaac gaaaaggagt
    12241 gaaacgaaaa tgtatggtta tggaatgtat ggttttttag atccgacatt tcttttggtc
    12301 attcttggat tagttatttc cggaattgcg tcagcgtacg tcaatagtac tttccgaaaa
    12361 tacgatcaag tgagaagcaa aaacaatgtg actggtacac aagcagcaca atatatatta
    12421 caaagtcaac agataaataa tgtcggtgtt caacaaattg ccggagatct cacagataac
    12481 tacaattcag gtaataagat gttgagtttg tctcaagcaa ctgcacagtc tacttcagtt
    12541 gctgcgatag gtgtagcagc tcatgaatgc ggacatgcgg tacaagatgc ttcgggttat
    12601 gtcccattga agattcgtgc agcacttgtt ccagcagcta actttggttc gatgatttcg
    12661 ttccccttga ttatggtagg tgtattgctt agctggaact ctacgttaat caatatcggg
    12721 attttcgctt tttctttagc attacttttc caattggtga cattaccagt cgaatttaac
    12781 gcgtctcggc gagcaatcca aattttgagt gaaggcggac tactgacgga agaagaagta
    12841 ccgatggcaa gacacgtatt atttgcagca gcactgactt atgttgcagc agcgctttcg
    12901 acattcctgc agctgttgcg cttgatctta ttgttcggcg gcaaccgaag agattaaaat
    12961 aaagaagttg tgaaatcagc gctttgatct gagctaatac cgagagttag ctgattgaat
    13021 accatttatt tggaataagg gctctttgtc aataaggact gatgggttgt acaaaatcaa
    13081 atctgggtca gaatgaacct cattctggct cag
//
LOCUS       DACDKM010000079        12919 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20834_2016
            SAMN12640370-rid9891663.denovo.079, whole genome shotgun sequence.
ACCESSION   DACDKM010000079 DACDKM010000000
VERSION     DACDKM010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640370
            Sequence Read Archive: SRR10040891
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12919)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12919)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742835.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742835.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:04:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,012
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 29 of 75
            Pseudo Genes (incomplete)         :: 33 of 75
            Pseudo Genes (internal stop)      :: 19 of 75
            Pseudo Genes (multiple problems)  :: 5 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12919
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640370-rid9891663.denovo.079"
                     /isolate="E20834_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(548..910)
                     /locus_tag="IV334_002020"
     CDS             complement(548..910)
                     /locus_tag="IV334_002020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8944079.1"
                     /translation="MASKRVAGTIMLHLEDGTKKFLVHSIDDKLEFALAELSEGKTGL
                     ANILNFLKEIVHIDVSKISLMELTNTHIKEENVPLFVFETEQKNQREELGEGYIWEEP
                     GQMRSVLGTYEVEGVPFF"
     gene            complement(984..1364)
                     /locus_tag="IV334_002021"
     CDS             complement(984..1364)
                     /locus_tag="IV334_002021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HAP8944080.1"
                     /translation="MTYKIGQIIEGKVTGIQPYGAFVSLDNETQGLIHVSEVQSGYTK
                     NIHSLLKVGQPVTVQIIDIDEYSKKISLSLRTLEKTNPAVPYRRKRYFTNKNRKIGFT
                     TIAEQLPIWIEEALSELERKENKK"
     gene            1446..2621
                     /locus_tag="IV334_002022"
     CDS             1446..2621
                     /locus_tag="IV334_002022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HAP8944081.1"
                     /translation="MIEFDKQISRKDTESVKWDAIAETYQMDDLLPLWVADMDFLSPG
                     GVAKAFSEYIKHGIFGYATLSDKLYQAIIHWERQHHAVELTKENIVFTSGVLTSLAAA
                     VQTFTKPGDSILIHDPVYPPFSSIIETNQRHIVRSSLLEKNNHFIMDLSDMEKKIEEN
                     NVKAMILCNPHNPGGRVWSKEELKQLSELCLKHQVILFSDEIHQDLTLFDHTFTSMLT
                     IDPNLDDLLVAFTSATKTFNLAAIKNSMVFIKNPEMKADFEKHLVMNQQHEINTFGLI
                     GTQAAYETGGEWLSQLIPYLEKNVQTVNQFFEQYLPKVRIMNPEGTYLMWLDFTAYIS
                     DDQELENTLVRKGKVVLNPGITFGPSGHGHMRLNIACPEETLLEGLNRIKYALETID"
     gene            complement(2698..3282)
                     /locus_tag="IV334_002023"
     CDS             complement(2698..3282)
                     /locus_tag="IV334_002023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HAP8944082.1"
                     /translation="MAFPQLDLKSEKGPKAVIKTNRGDITVQLFPELAPKTVQNFIEL
                     SKKGYYDGVIFHRVIPDFMIQGGDPTGTGMGGESIYGESFEDEFSRELFNLRGALSMA
                     NSGPNTNGSQFFIVNNTNVPANMLGQLEGAGFPSEIIEAYKGGGTPWLDFRHTVFGHV
                     LEGMDTVDEIANVQRGPQDRPVHDVVIETIEISE"
     gene            3483..4481
                     /locus_tag="IV334_002024"
     CDS             3483..4481
                     /locus_tag="IV334_002024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HAP8944083.1"
                     /translation="MEIYDITIIGAGPVGMFAAFYAGMRQAKTKIIDSLPQLGGQLAT
                     LYPEKYIYDIPGYPAIKASELIDQLEKQLTTFNHTFHLKEEVLSLTREDEVIEITTNK
                     GIHYSKAVILALGNGSFQPRKLNLDNAESFENHGLDYFVNDLMKYAGKKVAIAGGGDS
                     AIDWTLMLEPIASEVYLIHRRPEFRAHEHSVSRLKSSSVNLLTPYLIDGLSGNNGELT
                     DIRLKKVKSDETIDLMIDSLIVNYGFSSNLEHLSSWGLDSTRNAITVKSDMSTSIPGV
                     YAAGDICTYEGKVKLIATGLGEAPTAVNNALHYINPKERTQPGHSTSLYDKNLRPS"
     gene            4593..5102
                     /locus_tag="IV334_002025"
     CDS             4593..5102
                     /locus_tag="IV334_002025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HAP8944084.1"
                     /translation="MVVKTETLEEKARELLKERGVTVEDIAQLVMFLQKDYISDLTLA
                     DCTESVNSVLTKREVHNAIITGIQLDILAEKKKLMSPLQHIIEDDEGLYGIDEILALS
                     IVNVYGTIGFTNYGYIDKVKPGILKELNSHDGVHVHTFLDDIVGAIAAAAASRLAHKE
                     PEKRSRLTQ"
     gene            complement(5132..5791)
                     /locus_tag="IV334_002026"
     CDS             complement(5132..5791)
                     /locus_tag="IV334_002026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01906 family membrane protein"
                     /protein_id="HAP8944085.1"
                     /translation="MKNSRWQWMEYAGLFSLFLTLISLAVGVTINFRPLYVFDIGHLQ
                     ILDYTSLDQETLLKNFDHLMNYLNNPFKTILSLPDFPVSASGAHHFYEVKILFLVDYA
                     VFFITLIPSILFIKYLQKNDRLWRLIRPFQIGMLLPVVFGFFMMIGFDRFFILFHETF
                     FNNDDWLFDPVTDPIINVLPEEFFMHSFILFFVLLELFFAVFLFLGKNSLKQTKKKEL
                     V"
     gene            complement(5793..6557)
                     /locus_tag="IV334_002027"
     CDS             complement(5793..6557)
                     /locus_tag="IV334_002027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01457 family HAD-type hydrolase"
                     /protein_id="HAP8944086.1"
                     /translation="MDYKGYLIDLDGTIYRGTQPIPAGKRFVEELQKRKLPFLFVTNN
                     TTKSPETVANRLADEFDIHVAPETVYTATLATIDFMKADGKGKKVYVIGEAGLIDLIL
                     AAGFTWEEEAPDYVVVGLDNYLTYEKVVKATLAIQKGATFIGTNPDKNIPTERGLLPG
                     AGSVISFVETATQTPPIYIGKPEAIIMDKAVEVLGLQKEEVIMVGDNYETDIQAGIRN
                     NIDTLLVLSGFTKEEDVPGLPIPATYVKQSLDEWSF"
     gene            complement(6565..7242)
                     /locus_tag="IV334_002028"
     CDS             complement(6565..7242)
                     /locus_tag="IV334_002028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YutD family protein"
                     /protein_id="HAP8944087.1"
                     /translation="MSAKQNEQQNVEHKTEIAKKKSSAEQETTVTEELTAALEEIVEE
                     PKAEKVKGELVTLLDDSNFLIGERHYRLVSDYREGFNAEKLGERYSDVLARYDYIVGD
                     WGYEQLRLKGFFQADNRRAHPDQRIDSLEDYLYEYCNFGCAYFVIERIGGKKEKTTQR
                     RKKKKNHNRAYIDEKKGPVASNRKKPVIKNRKTESAKTSEKVKKQEQPKQVEKTKTAF
                     TIRKREE"
     gene            complement(7279..8664)
                     /locus_tag="IV334_002029"
     CDS             complement(7279..8664)
                     /locus_tag="IV334_002029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional metallophosphatase/5'-nucleotidase"
                     /protein_id="HAP8944088.1"
                     /translation="MEKIVILHTNDLHSHLENWPRIRRFLDQRKRENEKKEKTTTIAV
                     DLGDFVDRWHPLSEATNGQENILLMNEVGYDAATIGNNEGVGNSKEELNHLYDHAEFD
                     IILDNLFDKNTLQPPAWTKPYKIITTKHQTKIGLIAFTAPFPLTYNPNGWDIRNPYDI
                     LPELVEDLRSQVDVLVLMSHLGIQDDFQIAKEIPAIDVILGSHTHHLFREGHVINDVQ
                     IAAAGKYGQYVGEVHLTIDEEKKILKHSAKAIPTETMTAFTEDEQEINSYLERGHELL
                     KEKEVADLPFSLSLDIFNEHSFIQVAFEAVKERGKTEAAILNSGLFLTEIPKGRVNQD
                     QLHTALPHPMHLLNVTLGGNDLIRLVLEIEKNRNFLRNYPMKGMGFRGKIFGQMVYNG
                     ITYDSINHQVFWKNKPIDLNKNYTFTTVDHLMFVPFFPTIEIAGKNEFLFPEFIRSVV
                     GDYLKAHYPIK"
     gene            complement(8874..8958)
                     /gene="ffs"
                     /locus_tag="IV334_002030"
     ncRNA           complement(8874..8958)
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="IV334_002030"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            complement(8981..9496)
                     /gene="tadA"
                     /locus_tag="IV334_002031"
     CDS             complement(8981..9496)
                     /gene="tadA"
                     /locus_tag="IV334_002031"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HAP8944089.1"
                     /translation="MVNTNLDAVEKEKWMRLALAEAKKAEMLHEVPIGAVVVLDGKVI
                     GRGYNLRETTQDATTHAEMLAIKEACEKVGSWRLEDAALFVTLEPCPMCSGAMILSRV
                     AEVYFGAYDPKGGTAGTLMNLLEDERFNHQAYVEGGILEDECGMILTDFFRKLRQRKK
                     KLKKDWSTNEK"
     gene            9603..10460
                     /locus_tag="IV334_002032"
     CDS             9603..10460
                     /locus_tag="IV334_002032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fis family transcriptional regulator"
                     /protein_id="HAP8944090.1"
                     /translation="MKMDKLLALYPQAQKKNSPASEADILSFAIDDAFIWIKQDSLSQ
                     QETSLLKALFPVINDQKKHPWYRYLFKDASCVQEKSFRLIQLHVESKGEFLKKEWQDT
                     ILELFRYSADFFFYTDTDALLVEQINRDYLDATEINGIFLSLDADFDTTTSVFVGSFH
                     SPGTDLVPLFAEERKIFLTEQNNLYTHSRTFSMQDVALHYYTKDTVKDSKLIKSYIEL
                     IKQNEMEVIIQELWNELGNVSSAAKTLFLHRNTLKYRIEKFQEQSGFNLKKANDLLFC
                     YLLLLHEHN"
     gene            complement(10541..10876)
                     /locus_tag="IV334_002033"
     CDS             complement(10541..10876)
                     /locus_tag="IV334_002033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8944091.1"
                     /translation="MKTSTKVTIGLSVAAAASVATAVVVSGKVMEKIHHMTTRTKVKK
                     FVYDKFDGNEKLLEIVDGLSDSDLDSLMGMLAKIKSGKKKISVYGDSIKDSTEDVKDR
                     LMHFVEKMM"
     gene            complement(10943..12313)
                     /locus_tag="IV334_002034"
     CDS             complement(10943..12313)
                     /locus_tag="IV334_002034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NOL1/NOP2/sun family putative RNA methylase"
                     /protein_id="HAP8944092.1"
                     /translation="MKEEATTLPQQFIKKYRLLLGEEASDFFSALEQGSVKKGFRWNP
                     LKPAGLDMVQTYHSEELQPAPYSNEGFLGTVNGKSFLHQAGYEYSQEPSAMIVGTAAA
                     AKPGEKVLDLCAAPGGKSTQLAAQMKGKGLLVTNEIFPKRAKILSENIERWGVSNAIV
                     TNHAPAELVPHFSGFFDRIVVDAPCSGEGMFRKDPNAIKEWTEESPLYCQKRQQEILS
                     SAIKMLKNKGQLIYSTCTFAPEENEEIISWLVENYPVTIEEIPLTQSVSSGRSEWGSV
                     AGLEKTIRIWPHKDQGEGHFVAKLTFHGQNQMHKEKKTRKKSKVQMTKEQEKLWTEFS
                     NDFHYEATGRLLVFNDHLWEVPELAPSLDGLKVVRTGLHLGDFKKNRFEPSYALALAT
                     KKIENIPCLPITQKEWQSYTAGETFQRDGNQGWVLLVLDKIPVGFGKQVKGTVKNFFP
                     KGLRFH"
     gene            complement(12391..>12919)
                     /locus_tag="IV334_002035"
     CDS             complement(12391..>12919)
                     /locus_tag="IV334_002035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAP8944093.1"
                     /translation="RVTNYSKEYLLFKSMVYVEEYGMKSITARELAEFCGCSTYPIYT
                     HFKSISGLKEKILEEITVYFENYLAECSSDDVLSIVYLLKDFFSIHESIREIIAKSDL
                     DMDSLFKRPFFSYVKGHTKIKEEKSLELVWLNALGSLRSDDSSQDFLEMMNAVWDKNE
                     VTDEYLENILKEQSP"
BASE COUNT         3829 a         2607 c         2159 g         4324 t
ORIGIN      
        1 ggagtgtttc ccgattcgtt tttggctgcc gtagcaactt ttatatcata tcagagagac
       61 aagcgattgt caactctttt ttaaaagttt ttttcgattt cgtttttcga aatctttcag
      121 caactcattt atcataactt atcgtttgtt atttgtcaag aacttttttg aagttttttt
      181 cgatagaatc aagcttcgca agtggttctt ttttaacgac tcagttatct tatcatgtcg
      241 ttagtaacat gtcaataact attttttaaa ttttttagaa atgtgatata acatccactt
      301 cagaatcaac gctgttaaat ttatcatgta tttcttcctt ggtcaactat aaattaagca
      361 ttactttgtg aaatgatttt tcgatcaatt attattattc gttatacaac cgttttcaac
      421 atattttgaa aataaaatgc actaaaaaac tatctttagc agaacatttc attgtatttt
      481 ttggaccaaa caataaaaaa agaagtttga aaaaaattca aacttctttg ttcgcttaat
      541 ctgtatttta gaagaaaggc actccctcta cttcgtaagt tccaagaaca gagcgcattt
      601 gacctggttc ttcccaaata tatccttcgc ctaattcttc tctttgattc ttttgttcag
      661 tttcaaagac aaacaacgga acattttcct ctttaatatg tgtatttgtc aattccatca
      721 aactaatctt gctaacatcg atatgaacta tttcttttaa gaaattaagt atattcgcta
      781 aaccagtctt tccttcagaa agttccgcta acgcaaattc caacttgtca tcaattgaat
      841 ggaccaaaaa ttttttagta ccatcctcca aatgcaacat aattgtacca gcaactcttt
      901 tgcttgccaa aatttccacc tcgatcaact tgttctatgg cttacataaa aattctagca
      961 cattttcaga aaaaatacac tttttatttc ttattttctt tccgttctag ttcagataat
     1021 gcttcttcta tccaaatagg taactgctcc gcaatagtgg taaatccgat cttacgattt
     1081 ttattcgtga aatacctttt tctgcgatat ggaacagctg gatttgtttt ttctaacgtt
     1141 cttaaagata aactgatttt tttggaatat tcatcaatgt cgatgatctg aactgttact
     1201 ggctgtccga cttttaacaa tgaatggata tttttcgtat aaccggattg tacttctgaa
     1261 acatgaataa gtccttgcgt ctcgttatca agagaaacga atgcgccata aggctgtatc
     1321 cctgtcactt ttccttcaat tatttgtcca attttataag tcatctactt ccctctcttc
     1381 gcatttatca ttatactgta aatagctcaa aaaaagaagc gacgaaaatg actggaggtc
     1441 ttaagatgat cgaatttgat aaacagattt caagaaaaga tacagaaagt gtaaaatggg
     1501 acgcaattgc tgagacatat caaatggatg acttacttcc tttgtgggta gcagatatgg
     1561 acttcttgtc tcccggtggg gtagctaaag cgtttagtga atacatcaaa catggaatct
     1621 ttggttatgc cactctgtct gataaactat atcaggctat cattcattgg gaacgccaac
     1681 accatgctgt ggagttgacg aaagaaaata tcgtttttac aagtggtgtg ttaaccagtt
     1741 tagctgcagc agtacaaact tttacaaaac caggcgattc tattttgatt catgacccag
     1801 tttatcctcc attttcctct attatagaaa caaatcaacg tcacatcgtt cgaagctctt
     1861 tgctagaaaa aaataatcat ttcatcatgg acttgtctga catggagaaa aaaatcgaag
     1921 aaaacaatgt aaaagcaatg attttatgca atcctcacaa tcctggaggg cgtgtttgga
     1981 gcaaagaaga attgaaacag ttaagcgaat tgtgtttaaa acatcaagtg attctgttta
     2041 gtgacgagat ccatcaagat ttaactttat tcgatcatac atttacttcc atgctaacga
     2101 ttgatcctaa tttagatgat ctactagttg cttttacttc cgcaaccaag actttcaatc
     2161 tagctgcaat caaaaattca atggtattta tcaaaaatcc agagatgaaa gctgattttg
     2221 aaaaacattt agtgatgaac caacagcacg aaatcaacac ttttggactg attggaaccc
     2281 aagcggctta tgagacgggt ggagaatggt taagccaact gattccttat ctagaaaaaa
     2341 atgttcaaac agttaatcag ttttttgaac agtatcttcc gaaagtgaga ataatgaacc
     2401 ctgaaggaac ttacttaatg tggttagatt ttaccgccta tatttcagac gaccaagaat
     2461 tggagaacac tcttgttcgg aaaggaaaag tcgtattaaa tcctggcatc acgtttggtc
     2521 cttccggtca tgggcatatg cgcttgaata ttgcttgtcc agaagaaact ttactggaag
     2581 gacttaatcg aatcaagtat gctttagaga caatcgatta aatcatttat tcaaaataaa
     2641 aggacgagac cttactaggt ttcgtccttt ttcataatta ctccgtacca taccaaacta
     2701 ttctgaaatt tcaattgttt caatcactac atcatgtaca ggtcggtctt gtggaccacg
     2761 ttgtacattt gcgatttcat ctactgtgtc catcccttct agtacatgtc caaagactgt
     2821 atgtcggaaa tctagccaag gagttccgcc acctttatac gcttcaatga tttctgatgg
     2881 gaatccagca ccttctagtt gtccaagcat attagctggc acatttgtgt tatttacaat
     2941 aaagaattgg cttccgtttg tgtttggacc tgaatttgcc atagataaag ctccgcgaag
     3001 gttgaacagt tcacgactga attcatcttc aaaactttcg ccataaatac tttcgccacc
     3061 cattcctgtt ccagttggat cgccgccttg gatcatgaaa tcaggaataa cgcggtgaaa
     3121 aatcacgcca tcataataac cttttttgga aagttcaatg aaattttgaa ccgtcttcgg
     3181 tgctagttca gggaataatt gaacagtaat gtcaccacgg tttgttttga taacagcttt
     3241 tggtcctttt tcacttttta aatctagttg tgggaatgcc atattgtttc ctcctaatat
     3301 ttgcatactt cataataata ataacataat acctaacctc tcgtaaaaat actagatttg
     3361 ttttctttta tttttcatca ctagatacat tctataactt accgtttcga tttcgaattc
     3421 aaacattttt gagaagcatt ctcaattaaa ttctgctata gtgtgaaaag ggaggaattt
     3481 ttatggaaat ctatgacatt acaatcatcg gtgcaggtcc tgtagggatg tttgccgctt
     3541 tttacgcagg aatgcgccaa gctaaaacaa aaatcattga cagtctacct caacttggtg
     3601 gtcaattagc aactttgtat cccgaaaaat atatttacga tattcctggg tatccagcaa
     3661 taaaagcaag tgaattaatc gatcaattag aaaaacaatt aactactttt aaccatacat
     3721 tccatttaaa agaagaagtt ctctcgctta ctcgtgaaga cgaagttatc gagattacta
     3781 caaataaagg catccattat tccaaagcag tcattttagc tttaggaaat ggttcattcc
     3841 agccaagaaa attgaattta gataatgctg aatcatttga aaaccacgga ttggattatt
     3901 tcgtgaatga tttgatgaag tatgctggaa aaaaagtcgc tattgctgga ggtggtgatt
     3961 ctgctatcga ttggacgttg atgttagagc ctattgcaag cgaagtttac ttgattcatc
     4021 gtcgcccgga atttcgtgcg cacgaacata gtgtatcacg cttgaaatct tcatctgtca
     4081 acttgcttac tccctatctc atcgatggat tatcaggcaa taatggggaa ctgacagata
     4141 tccggttaaa aaaagtgaaa agtgacgaga cgattgattt gatgattgat tctctgatcg
     4201 tcaattatgg attctcttct aatttagaac atctttcttc ttggggactt gatagtacca
     4261 gaaatgccat tactgtaaaa tcagatatgt ctacttctat tccaggcgtt tatgcagctg
     4321 gtgacatctg tacctatgaa ggaaaagtga aactgattgc tactgggctc ggtgaagcac
     4381 ctactgcagt gaacaacgct ttacactata ttaatccaaa ggaacgaact cagcctggtc
     4441 acagcacaag cttatacgat aaaaatcttc gtcctagttg attttttata tctctttgca
     4501 tttcaagtta aacccgaaag cagtttcttg catttttttc atgttccgtt aacatgaaat
     4561 agagaaagaa aaaataaagg gggagcaata atatggtcgt caaaactgag actttagaag
     4621 aaaaggcaag agaactattg aaggaacgtg gcgtaactgt agaagatatc gcacaacttg
     4681 tcatgttttt acaaaaagac tatatttctg atttgacttt ggcggattgt actgaaagtg
     4741 tcaacagcgt gctaacaaag cgtgaagtac ataacgccat tatcactgga atccaattag
     4801 atatattagc agaaaagaaa aaattgatga gtccgttgca acatattatc gaagatgacg
     4861 aaggccttta cggtatcgat gagatcctag ccttgtcaat cgtgaatgtt tacggtacga
     4921 tcggctttac gaattatggt tatatcgaca aggtgaaacc tggtatttta aaagaactaa
     4981 acagccacga tggcgttcat gtccatacct tcttagatga tatcgtcgga gcgatcgccg
     5041 ctgctgctgc cagtcgatta gcacacaaag aaccagaaaa aagaagtcgc cttactcaat
     5101 aattcaacaa aaaaagaaag aaaacgtcta tttagacgag ttctttcttt tttgtttgtt
     5161 tcaagctatt ttttcctaag aaaagaaaaa cagcaaaaaa taattctaat aatacaaaaa
     5221 ataagataaa gctatgcatg aaaaattctt ctggaagtac attgatgatt ggatcagtaa
     5281 cgggatcgaa taaccagtca tcgttgttga aaaacgtttc atgaaaaagg ataaaaaagc
     5341 gatcaaatcc tatcatcatg aaaaaaccga aaacaactgg cagtaacatg ccgatttgaa
     5401 acggacgaat caaccgccaa agccgatcat tcttttgcaa atatttgata aacaaaatac
     5461 tcgggatcaa agtgatgaaa aacacagcat aatccactaa aaacaaaatt ttcacttcat
     5521 aaaagtgatg ggctccgcta gcagaaactg ggaaatctgg caagctgagg atggttttga
     5581 agggattatt gagataattc attaggtgat caaaattttt cagtaacgtc tcttgatcta
     5641 aagaagtata atccaaaatt tgcaaatgtc cgatatcaaa tacgtatagt ggtcgaaagt
     5701 tgatcgtcac accaactgcc agactgatca gtgtcagaaa taggctgaac aatccagcat
     5761 attccatcca ctgccatctc gaatttttca tattagaagc tccattcatc cagtgattgt
     5821 ttcacatagg tagctgggat aggcaaccca ggtacatctt cttcttttgt aaaacctgat
     5881 aaaacgagca aagtatctat attattgcgt atcccagctt ggatatctgt ttcatagtta
     5941 tctccaacca tgattacttc ttctttttgc agccctaata cttcaacggc tttatccata
     6001 atgatggctt ctggttttcc gatgtagatc ggcggcgttt gggttgctgt ctctacaaaa
     6061 gagatgactg accctgctcc aggaagaagg ccgcgttcag taggtatatt cttatctggg
     6121 ttcgttccaa tgaaagtagc acctttttga atagcaagtg ttgctttaac tactttttca
     6181 taggtcagat aattatctaa gcctaccact acatagtcag gagcctcttc ttcccatgta
     6241 aatccggcag ctaaaatcaa gtcaatcaat cctgcttctc cgataacata gacttttttc
     6301 ccttttccat ctgctttcat aaaatcgatt gttgccaaag tagctgtgta gactgtctct
     6361 ggagctacat gaatgtcaaa ctcatctgct agacgattag ctactgtttc tggtgactta
     6421 gtcgtgttat tggtaacaaa caagaaaggt aattttcttt tttgcaattc ttccacaaat
     6481 cgtttaccgg ccggtatggg ttgtgtccca cgataaatcg ttccgtctaa atcaattaaa
     6541 tatcctttat agtccattca tccactactc ttcccttttt ctgatcgtaa atgctgtttt
     6601 tgttttttct acttgtttag gttgttcttg tttttttact ttttcagagg tttttgctga
     6661 ctctgttttt cgatttttaa taaccggttt tttgcgatta gatgcgactg gtcctttttt
     6721 ctcatcgata tacgcacgat tatggttttt tttctttttt cggcgctgag ttgtcttttc
     6781 ttttttaccg ccgattctct cgatgacaaa atacgcacaa ccaaaattgc aatattcgta
     6841 aagatagtct tccaaggaat cgattcgctg atctggatgc gcccgtcgat tatccgcttg
     6901 gaaaaatcct tttaggcgta gttgttcata accccaatct cctacgatat aatcatatcg
     6961 ggctagtaca tcactgtaac gttctccaag cttttctgca ttaaatcctt cacgataatc
     7021 ggacacaagc cgataatgtc tttcacctat caaaaaatta ctgtcatcta acagtgtcac
     7081 tagctcgcct ttgacttttt ctgctttagg ctcctcaacg atttcttcca aagcagctgt
     7141 cagttcttcg gtgactgttg tttcctgttc agctgatgat tttttcttag caatttctgt
     7201 tttatgctca acgttctgct gttcattttg ttttgcagac atgcgttcac cacctatctt
     7261 ttttattata ccttgttttt atttgattgg gtagtgagct ttcaagtaat ctcctaccac
     7321 acttcggata aattcaggga acaaaaattc attttttcca gcaatctcaa tcgttggaaa
     7381 aaatggaaca aacatcaaat gatctacggt agtaaaagta taatttttat tcagatcgat
     7441 tggcttgttc ttccaaaaga cttgatggtt aatagaatca tatgtgattc cattatagac
     7501 catctgccca aaaatcttac cgcgaaatcc cattcccttc ataggatagt tacgtaaaaa
     7561 gttacgattt ttttctattt ctaaaactaa gcggatcaga tcatttccac ccaatgtgac
     7621 atttaacaag tgcatcggat gcggcaacgc agtgtgaagc tgatcctgat tgacccgccc
     7681 ttttggaatt tcagttaaaa acagaccact gtttaatatt gctgcttccg tctttcctcg
     7741 ttctttcacc gcctcaaagg ctacttggat aaacgaatgc tcattgaaga tatctagact
     7801 cagagaaaat ggcaaatctg ctacttcttt ttcttttaat aattcatgcc cacgttctag
     7861 ataactattt atttcttgtt catcttctgt aaaagctgtc attgtttcgg tagggatggc
     7921 tttggctgaa tgcttaagga tttttttctc ttcatcgatg gttaaatgga cttcaccgac
     7981 atactgcccg tactttccag cggctgctat ttggacatca ttgattacgt gaccttctct
     8041 gaataaatga tgggtgtgcg atcctaaaat aacatcaatt gctggtattt cttttgcaat
     8101 ttggaaatca tcctgaatcc cgagatgact catcaaaact aaaacatcca cttgtgaacg
     8161 taaatcttcg actaattctg gcaaaatatc ataaggattc cgtatgtccc agccattcgg
     8221 attataagta agcggaaatg gagcagtaaa tgcaattagt ccgattttcg tctggtgttt
     8281 cgttgtaatg attttatatg gtttcgtcca tgccggtggc tggagcgtat ttttatcgaa
     8341 taagttatcc aggatgatat caaactcagc atgatcgtat aaatgattca actcttcttt
     8401 ggaatttcct acgccttcat tgtttcctat cgtcgctgca tcataaccaa cttcattcat
     8461 cagcaagata ttttcctgtc cattcgttgc ttctgacaat ggatgccaac gatccacaaa
     8521 atcacctaga tctactgcaa tcgttgtcgt tttttctttc ttttcgtttt ctcgtttacg
     8581 ttgatccaaa aaccttctga tccttggcca attttctaaa tgagaatgca gatcattcgt
     8641 atgtaaaatc actatttttt ccatgtaccc tctcgcctct ctttatcttt atcataccat
     8701 aggcagcttt ttcttccatt gaaacaagct ctatttcaga gtttttcctg ccttgaagca
     8761 aattgtataa gtacaatatt cgataaatca gtctggctaa aaaatcagga acattcattt
     8821 ttacagtact ataaaaataa aaaaaacaga cccgaaagtc tgttgtcgtt tcattataat
     8881 cagacacatg gcggaacctg cttagtgctg cttccttcca gacctgacac gcttcacaag
     8941 cccaccattg tcctagcctt tcggcaaaga atagtatacc ttatttttca ttcgttgacc
     9001 agtctttttt taatttcttt tttcgctggc ggagctttcg gaaaaagtca gtcaaaatca
     9061 tcccacattc gtcttctaaa atgccacctt ctacatatgc ttggtggtta aaccgctcgt
     9121 cctccaaaag attcatcaag gtaccggctg ttccgccttt tggatcatac gcgccgaaat
     9181 aaacttctgc tacacgagac aaaatcatcg cgccgctgca cataggacaa ggctctaagg
     9241 taacaaaaag tgcagcatct tccaagcgcc agcttcccac tttttcacag gcttccttga
     9301 ttgcaagcat ttctgcatgt gtagtagcat cttgcgtggt ttctctaagg ttatatcctc
     9361 ttccaatcac ttttccatcc aatacaacca ctgctccaat cggaacttca tgaagcattt
     9421 ctgctttctt tgcttctgcc aatgcaaggc gcatccattt ttctttttct actgcatcaa
     9481 gatttgtatt taccaattac ccttccctgc ctttgttttt tcttgtttta ctttagcata
     9541 tgataaaaag gctgtaacta actttttttt ctatggtaaa ataaatacaa gaggtgaaca
     9601 agatgaaaat ggataaatta cttgctcttt acccacaagc acagaaaaaa aactctccag
     9661 catcagaagc ggatatcctt tcttttgcaa ttgatgatgc ttttatctgg atcaaacaag
     9721 attcattatc tcaacaagaa accagccttt tgaaagcgtt attccctgta atcaacgatc
     9781 aaaaaaaaca cccatggtat cgctatcttt ttaaagatgc ttcttgtgtt caagaaaaaa
     9841 gctttcgttt gatccagctt cacgtcgaat caaaaggaga attcttaaaa aaagaatggc
     9901 aagatactat tttggaatta tttcgctatt ctgctgattt ctttttttat acagacacag
     9961 atgcgctctt agtcgaacaa ataaaccgtg attatcttga tgctactgaa atcaacggta
    10021 tttttctatc tttagatgct gattttgata cgacaacgtc tgtattcgtt ggttctttcc
    10081 attcacctgg caccgacctc gtccctttgt ttgctgaaga aagaaaaatt tttttaacag
    10141 agcaaaacaa tctttatact cacagccgta ctttttctat gcaagatgtg gctttacatt
    10201 actatacaaa agatactgta aaagatagta agttgatcaa aagttacata gaactgatca
    10261 aacaaaatga aatggaagtg atcattcaag aattatggaa tgaattaggc aatgtcagct
    10321 ctgctgcaaa aacactgttt cttcatcgta atactctaaa atatcgaatc gaaaaatttc
    10381 aagaacagtc tggtttcaat ctaaaaaaag ccaacgattt actgttttgc tatcttttat
    10441 tgcttcatga acataattaa agatagatta aaggggttgt gacaataatt ttgtcacaac
    10501 cccttatttc gaatgaatag tgttcaaaag ctagtttctc ttacatcatt ttttcaacaa
    10561 aatgcattag ccggtctttt acatcttcgg ttgaatcttt gattgaatca ccataaacag
    10621 agattttctt ttttcctgat ttgatttttg ctaacattcc catcaaagaa tccaagtcac
    10681 tatctgaaag accatctacg atttctagta atttttcatt tccatcaaat ttgtcataga
    10741 cgaatttttt tactttcgtc cgagtagtca tatgatggat cttttccatt acttttcccg
    10801 atacaacgac tgctgtcgct acactggcag cggctgctac gcttaatccg attgttactt
    10861 ttgttgatgt tttcataagt atcagctcct ttttatctat ctccatcata gcatagacaa
    10921 aaaaataaac gaactaatcc actcagtgaa atctcaatcc tttagggaaa aagttcttta
    10981 ctgttccttt tacttgtttc ccaaatccga caggtatctt atctaatact agcaaaaccc
    11041 atccttgatt cccatcacgt tgaaatgttt ctcctgctgt gtaagattgc cattcctttt
    11101 gagtgatcgg caagcaaggt atattctcta tcttttttgt agcaagcgct aatgcatagc
    11161 taggttcaaa tcgattcttt ttgaaatctc ctaaatgcaa acctgtacgt acgactttta
    11221 aaccatcgag agaaggggct aactcaggaa cttcccaaag atggtcgttg aacactaaaa
    11281 gtcttccagt agcttcataa tgaaaatcat ttgaaaactc cgtccagagt ttttcctgct
    11341 cttttgtcat ttgcacttta cttttttttc gtgtcttctt ttctttatgc atctgatttt
    11401 gtccatgaaa agtcagcttt gcaacaaaat gtccttcacc ctgatcttta tgaggccata
    11461 tacgtattgt tttttccaaa ccagctacgc ttccccattc agaacgtcct gaactaacgg
    11521 attgagtcaa aggaatctcc tcaatcgtta ctggataatt ttctacaagc cagctaatga
    11581 tctcttcatt ttcttctggt gcaaacgtac aagtcgaata aatcagttgt cctttatttt
    11641 ttagcatttt tattgctgaa gacaaaatct cttgctgacg tttttgacag tatagcggag
    11701 attcttctgt ccactctttt attgcatttg gatcttttcg gaacattcct tctcctgaac
    11761 aaggagcatc aaccacgatc cgatcaaaaa agcccgaaaa atgagggaca agttctgcgg
    11821 gtgcatgatt tgtcactatt gcgttcgaaa ctccccatcg ttctatattt tctgataaaa
    11881 tcttcgcacg tttcggaaag atctcattcg tcactaataa ccctttcccc ttcatttgag
    11941 cagctaattg ggttgattta ccaccgggag ccgcacacaa atccaaaact ttttcaccgg
    12001 gttttgctgc agcagctgtt ccaacgatca ttgcacttgg ttcttggcta tattcatacc
    12061 cagcttgatg gaggaaagac tttccattga cagtgccaag aaatccctca ttgctgtagg
    12121 gagcgggttg taattcttcg ctatggtaag tttggaccat gtccaatcct gccggtttta
    12181 gtggattcca ccgaaatcct tttttcacgc ttccttgttc aagcgcagaa aaaaaatcgg
    12241 atgcttcttc gcctaaaaga agacgatatt ttttgataaa ttgctgtggt aacgttgtcg
    12301 cttcctcctt cattccaatg tttcgttaga cattttacaa tcttttttac gaaaaataaa
    12361 ggtggctgat ctactttttc atgttgctgc ctaaggtgac tgttccttta agatattttc
    12421 caagtattca tccgtcacct cgtttttatc ccaaacagcg ttcatcattt caagaaaatc
    12481 ttgagaagaa tcatctgagc gcaaacttcc caatgcatta agccatacca attcaagcga
    12541 cttttcttct ttgatcttcg tgtgaccttt gacatatgag aaaaatggcc gcttaaatag
    12601 cgaatccatg tctaaatcag attttgcaat gatttcacga atactttcat ggatagaaaa
    12661 aaaatctttc aataagtaga caatgcttaa aacatcatcg gaactgcatt cagctaagta
    12721 gttttcaaaa taaacagtga tttcttctaa tatcttctct tttaacccgc tgattgattt
    12781 gaaatgtgta taaatcgggt atgtagaaca accgcagaac tctgctaact ctcttgcagt
    12841 aattgacttc attccatatt cttccacgta taccatactt ttgaacagca aatattcttt
    12901 actataattt gttactctc
//
LOCUS       DACDKM010000080        12811 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20834_2016
            SAMN12640370-rid9891663.denovo.080, whole genome shotgun sequence.
ACCESSION   DACDKM010000080 DACDKM010000000
VERSION     DACDKM010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640370
            Sequence Read Archive: SRR10040891
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12811)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12811)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742835.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742835.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:04:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,012
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 29 of 75
            Pseudo Genes (incomplete)         :: 33 of 75
            Pseudo Genes (internal stop)      :: 19 of 75
            Pseudo Genes (multiple problems)  :: 5 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12811
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640370-rid9891663.denovo.080"
                     /isolate="E20834_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(<1..715)
                     /locus_tag="IV334_002036"
     CDS             complement(<1..715)
                     /locus_tag="IV334_002036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS982 family transposase"
                     /protein_id="HAP8944094.1"
                     /translation="MRSSKYTEHYTDTFITFKEIMRTVSNIYHNCVPDKIKNRRNTDQ
                     LKQRDTVIIACVIWGIINGYTSQRATYRAVCSVLFPNGDFPSRSRFTRLSSNLAYTIK
                     IIRYFFIKKLTKGELVGIIDSFPSPLCKPVRNRQAKLLNQIAKVGYNSTKKSYFYGLK
                     IHMIVTKTGFPVTYSITNPGVHDVKVLETLSEEANLPNILGDKGYIRHKIHEKLALKG
                     ITISVPPRKNMDKSEKLDHS"
     gene            1142..1921
                     /locus_tag="IV334_002037"
     CDS             1142..1921
                     /locus_tag="IV334_002037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8944095.1"
                     /translation="MEKPFVKSLTAVAVGAGAIAICLFGYHINNQRQHQQRINYAESA
                     ITNQKDTLTSLSKEVDKLYSTKEKIFLNPEITEETITSLSNKLSSVKLSADDYDIKES
                     ELPKEAVAIQDEKKAIVTQLDSAESKLKIQTAVNDLFTKNVSNWQQAVDDVIIKEKVT
                     SADVARVRENMSFFKDSPWKTVVMQYLGFADIQIAQVTQLDQLFDTMLKDGQVTATAT
                     YDQYLTVLSQIEQIRNEKISAAYATKAQTVAEQMGYSNISY"
     gene            1967..2911
                     /locus_tag="IV334_002038"
     CDS             1967..2911
                     /locus_tag="IV334_002038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAP8944096.1"
                     /translation="MARRRRKSRGKKIFLIILAVLIVLVLAIVGVGAKLYLDLSHSIE
                     KTYESVERTQGERTKPVDLDKQESFSVLLMGIDTGDLGRVDQGRSDTMMVATVSPEDK
                     QTTIVSIPRDTYVDIVGRDTKDKINHAYAFGGAAMAMDTVQKYVDIPIDHYVSINMKG
                     LKELVDAVGGIEVNNDLTFSQDGYDFPIGKITLNGDQALSYSRMRYEDPNGDYGRQGR
                     QRKIVEGVAKKVLSLNGVSNYQSVLNALESNMKTDMNFDEMKKIALDYREAFGTIKQD
                     QLQGEGFMQDNISYQKVSDQELDRVKKELKDQMNLENK"
     gene            2927..3706
                     /locus_tag="IV334_002039"
     CDS             2927..3706
                     /locus_tag="IV334_002039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine protein kinase"
                     /protein_id="HAP8944097.1"
                     /translation="MEETISLKEIFETLRKHLVIIIISMFAGLAISGIVTFFILTPKY
                     SSQAQLIVTLPQSDTANVNDVNTNLQMINTYKDMIVSDLVLNEVKDRLETEDNVKMTA
                     EQIKDAISVNQAENSQMFSIQAISTNAVAAQQIANTTSQVFQENAKDVMNVDKISIIS
                     GAVANTTPVSPNNKLNLMTGLMLGMMVGVGLAFLLELLDRTVKDEKYVTDTLGFPILG
                     TVPEMSAKELNAGIRKKGPAQTQPKATNDKTAPSRRSRSRV"
     gene            3718..4416
                     /locus_tag="IV334_002040"
     CDS             3718..4416
                     /locus_tag="IV334_002040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CpsD/CapB family tyrosine-protein kinase"
                     /protein_id="HAP8944098.1"
                     /translation="MARTQKQKAAQTHAVSLVTLTNPSSPIAEQYRTIRTNIQFASAA
                     GQQIKTIVVTSSGPGEGKSTTAANIAVVFAKSGQRVLLVDADLRKPVIYKTFKLNNAS
                     GLSTVLSSSGSVADEIQRTPVDNLSILPSGPKPPNPSELLSSPRMDQILAEARQLFDV
                     VIFDMPPVVAVTDAQIMSSKTDGTLLVVRENTSRKESLTKAKELLDMVQARVLGVVYN
                     GAEHSKDAGYYYGN"
     gene            4444..5208
                     /locus_tag="IV334_002041"
     CDS             4444..5208
                     /locus_tag="IV334_002041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine protein phosphatase"
                     /protein_id="HAP8944099.1"
                     /translation="MIDLHCHILPGIDDGAENLEASIAMAEKAIQQGITHILCTPHHN
                     NGKYSNEKSQVISLVASLQAELEKRQLPLTLLEGQEVRITGTLIEDIHRDEILFTDLD
                     DTYLLIEFPTLEVPLYAERLFFELLELGKIPVIVHPERNAHFREDPNHLLPFLDMGCL
                     AQLTAPSYVGVFGKDIQKTAKTMVKHNLVQMAASDAHGVSKRTFYLKECYEQIAKEFG
                     KEKVVQMKQVAKDLINGDEINYPIYEEVKKKKFGLF"
     gene            5252..6703
                     /locus_tag="IV334_002042"
     CDS             5252..6703
                     /locus_tag="IV334_002042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HAP8944100.1"
                     /translation="MYRKNSSGWSKHIDFILLDLFCVQLAFYISYVMRQGDWNPYVIP
                     IYRHMAIFIELENLTIIFLFEAYKNVLKRGYYKEAVASIKQSFMLLLVCSLYLITMQD
                     GNDYSRVSLFIMGVVYGLLTYIIRILWKGLLHHRMKNGEDVSLVIVTSQKIAKNVVRN
                     LKEKNYGMYKLSGVIVVDRDMYGTTIGGVPVVANADNAAEYLLQNWVDEVFINVDESV
                     PYPKELIARCSEMGLAVHMNLTKVSDSTRGKQFIGRVGDYTVITSSINVMTMRQAFIK
                     RTIDILAGIIGCIATGVIFIFVAPVIYIKSPGPIFFAQERIGQNGKTFKMYKFRSMYM
                     DAEERKAELMKENKMSNNLMFKMDFDPRIIGNKILPDGTKKTGIGQFIRSTSLDEFPQ
                     FYNVLMGKMSLVGFRPCLKSEYEKYDFHHRARIAMKPGITGMWQVSGRSDITDFEEVV
                     KLDAEYIRNWSMGLDFRILFRTVKTVLHRNGSM"
     gene            6735..7871
                     /locus_tag="IV334_002043"
     CDS             6735..7871
                     /locus_tag="IV334_002043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HAP8944101.1"
                     /translation="MARKKLKICMLGHKRIPSREGGIEIVVEELATRMVALGHEVTCY
                     NRSGHHVSGKEFDQHKNKEYKGVKLKTIFTLNIKGIAAMSSSVFGGIRAAFGKYDVVH
                     FHAEGPCAMLWLPKLFGKRCVATIHGLDHQREKWNKLASTYIMLGEKCAVKFADEIIV
                     LSESVQNYFEDIYGRKTRFIPNGVKKIEIKSAGLITEKYGLTKDSYILFLGRLVPEKG
                     IRYLIEAFKDVQTDKKLIIAGGSSDTDEFANELKELAKGDERIIFTGFVQGQELEELY
                     SNAYIYTLPSDLEGMPLSLLEAMSYGNCCIVSNISECTEVVEDKAMIFKKSDVSDLKI
                     RLEEACNQSEMVKVLKNQATEFICSKYNWDKIVQETLNLYRGNI"
     gene            7871..8977
                     /locus_tag="IV334_002044"
     CDS             7871..8977
                     /locus_tag="IV334_002044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HAP8944102.1"
                     /translation="MIRILQCVNDMHRAGLETMLMNYYRNIDRTKIQFDFLTHRPYKS
                     DYDEEIISLGGKVYYAPRLYPQNYPKYFKWMEQFFKDHPEYEIVHSHIDAMSYLPLKA
                     AKKAGIPIRIAHSHNTSIDRDFKYLLKQYYRCKLTSVLTHEFSCGKVAGDFLFRNDKF
                     TLIPNAVDAKKFYYDESIRNDVRMKLGISSQTFVMGHIGRISYSKNHRFLIEIFKEFH
                     AIKNNSVLLIIGTGDMEEEIKNYAKKSGIDDDIKFLGNRNDIEKFYQAFDVLMLPSLF
                     EGVPLVGIEAQFADLPCFFSEKVPTEVAFSDKTNFISLNQSAKEWAREIANVDISHRD
                     SERSILIKADYNIQTAYKILESKYYELFELIQRG"
     gene            8983..10005
                     /locus_tag="IV334_002045"
     CDS             8983..10005
                     /locus_tag="IV334_002045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HAP8944103.1"
                     /translation="MTQKHSSIKRIIHRISEELNATRRIAKITSWKEAFATLQAKIDI
                     QVMNRNGYNESKKQKEHLLKKHEVMLKYYEKTFSDFLKTYDYSQNQIINQSKSPYSNC
                     IWVCWWQGLDQSPEIVKACVKSIKRNAGKHTVIILTEDNYRQYVDIPKWVEEKKNKGI
                     ITRTNYSDLLRLSLLAKHGGLWLDATFYCTKPIPDEYFDIDIWSIRRPDYAHASVASG
                     YFAGYSLACNQESRWMFAIIRDFFLNYWKNNDMMVDYLMIDYMIVLAQRINHEIAQTF
                     DKIPSNNPLCDELIKNLNNIYDEKVWNLLKKDTLLFKLTWKQSFKKQKDDKVTFYGML
                     VENKLL"
     gene            10027..11082
                     /locus_tag="IV334_002046"
     CDS             10027..11082
                     /locus_tag="IV334_002046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HAP8944104.1"
                     /translation="MKHKVSVIVPIYNVEKYLRKCVDSLLSQTLKEIEIVLVDDGSPD
                     ASGEIADEYQKKYSNVKTIHRENGGLGPARNTGIENATGEYVAFLDSDDWVESDMYEK
                     LYLVASKENADIVVSGHCDFANDKAMVKKQHPLSGNVYDTPNSIKNVRKNLFGHSPKD
                     QEVEAFPMSVCMSLYNKNLLDKYRLRFEKILSEDTIFNLNAYDVANKIAFTEYTDYCY
                     RKEEQESITKSLSPNKQTEFKEFLITLKAIASKEDLECQIRAKRMAIDYCRLYVGLVD
                     DANLGIKEKRKYIKKFIEDEGLIQCWKGYPTNTLPFQQKFFHKMLECKCYTILLLLSR
                     IRKIIKNMTRKKLFIIL"
     gene            11700..>12811
                     /locus_tag="IV334_002047"
     CDS             11700..>12811
                     /locus_tag="IV334_002047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8944105.1"
                     /translation="MSVKKMLFMVSTIIVLTSRFWGINTSLSMRYFIMAIWDLYFMLS
                     YFSYREKTYKKYVIKNNFWLTIMPFVIFSIYTLIIWGIGSNAEFENFIKLCSTLLYLI
                     LAYGYACTAFYLFKAEGIDFIFWSGVISYMSGSVFYLIVSYGLNGLISYIKSLITGET
                     NTANFAMEVHDLTFAMGVFFLYYVFFENKNTKKHKIKIIVSLILIFMGLKRIELLALS
                     GAILVYYVLLKWGKKIQIRALVCTICATVISLVFIYIIDTGILALLMSNLGITDDGAR
                     LSFYLYSSKFYELGLGYVGTGWTWFSKYFFNLYLSHFRLNGGRIAASLHSDILTIYIE
                     VGCIVFIIWMFWLFFVKPIRLTKKFGLKTGECVLLLT"
BASE COUNT         4591 a         1749 c         2550 g         3921 t
ORIGIN      
        1 agctgtggtc tagtttttct gatttatcca tgtttttacg tggcggaacg gatatagtaa
       61 tgcccttaag ggcaagcttt tcatggattt tatgcctaat atatcctttg tcccctagga
      121 tattgggaag attcgcttct tccgataaag tctctaacac tttcacgtca tggacacctg
      181 gattcgtgat tgagtaagta accggaaagc cagtcttagt aacgatcata tgaattttaa
      241 gaccataaaa ataagatttt tttgttgagt tatagccaac tttggctatt tgattcaata
      301 gttttgcttg tctatttcta actggtttac ataatggact aggaaaacta tctataattc
      361 cgactagttc acctttggtg agcttcttga taaaaaagta tcgaataatc ttgatagtgt
      421 aagctaagtt tgaacttaag cgagtgaatc gactcctact aggaaagtca ccattaggaa
      481 ataagacgga acaaaccgct ctatacgtgg ctctttggct agtatagcca ttaattatgc
      541 cccatataac acaagcaata atcactgtat cgcgttgctt cagttgatca gtatttcttc
      601 gatttttaat cttatctggt acacaattgt ggtatatgtt agaaacagtt ctcataattt
      661 ccttgaatgt tataaaagta tctgtataat gttcagtata ttttgatgaa cgcatatgta
      721 gtcctcattt cttgggagtt ttgagactac tatatgcgtt tttttgttga tttaccaaca
      781 tgtttagaca ttttcctcta ggaaaaaatt aactagcacc acgggtataa ctattattaa
      841 ttcaatgtta atttttttgt tattctattt tttgtgtaac ttttaaaaac acctatgaaa
      901 attatggaaa taaaaatgaa atcgattaat ttttatctta ttaatgtttt atttactttt
      961 aaatcgtaaa ttgccctagt caagaaaaca gtttgtggta tgattatata gggaaaactt
     1021 gtttacaaat atttctgtga atatattagt ttaattatgt ttttttaaat aaaaagctgt
     1081 catttttatg taagtgaatt atatcttaat ttatctatca tcaatactta ggagtgaatc
     1141 tatggaaaag ccattcgtca agtcattgac tgctgtggcg gtaggtgcag gtgctattgc
     1201 gatctgtttg tttggatacc atatcaataa tcagcggcaa catcagcaac ggatcaatta
     1261 tgcagaatct gccatcacca atcaaaaaga caccttgact tctttatcaa aagaagtaga
     1321 caagctgtat agtacaaaag aaaaaatttt tctaaaccct gaaataacag aagaaacaat
     1381 cacaagtttg tcaaataagc tatcctctgt caaactttca gcagatgatt acgacatcaa
     1441 agaatcagaa ttgcctaaag aagctgttgc aatccaagat gagaagaaag caatcgtgac
     1501 acagttagat agtgcagaat caaaactgaa gatccagaca gctgtcaacg atttatttac
     1561 taaaaatgta tcaaactggc agcaagctgt agacgatgtc atcatcaaag aaaaggtgac
     1621 aagtgcagat gtcgctcgtg tacgtgaaaa tatgagcttt tttaaagata gtccttggaa
     1681 aacggttgtt atgcaatatc taggattcgc tgatattcag atcgcgcaag tcacgcaact
     1741 agatcagtta ttcgatacga tgttaaagga tggtcaagtg actgccaccg ctacttacga
     1801 tcaatatttg acggtgctca gtcaaataga gcagatccgg aatgaaaaaa taagcgcagc
     1861 atacgcaaca aaagcacaaa ctgttgctga gcaaatgggc tatagcaata taagctattg
     1921 acagttaaaa cgttgattca agcaaggtta gcaaacggag gaaactatgg caagacgaag
     1981 aagaaaatca agagggaaaa aaatatttct gattatttta gcagttctta tcgtattagt
     2041 attagcaatt gtaggagtag gagcaaaatt atatctggat ctctcacatt ctattgagaa
     2101 aacttacgaa tcagtggaac gaacccaagg agaacgaacg aaaccagttg atctggacaa
     2161 acaagaatct ttctctgttc tattgatggg gattgatact ggagatttag gtcgggtcga
     2221 tcaaggaaga tcagatacga tgatggttgc cacagtcagt cctgaagaca aacagacaac
     2281 gatcgtcagc attcctcgtg atacgtatgt cgacatcgta ggtcgtgaca caaaagacaa
     2341 gatcaaccac gcttacgcat tcggcggagc tgccatggcg atggataccg tacaaaaata
     2401 tgtagacatc ccaatcgatc actatgtctc tatcaatatg aaagggctaa aggaactcgt
     2461 tgatgcagtt ggtggtatcg aagtcaataa tgacctcact ttctcacaag atggctatga
     2521 ctttccaatc ggtaaaatta cattgaacgg ggatcaggca ttatcttatt ctcgtatgcg
     2581 ttatgaagac ccaaatggtg attacggtag acaagggcgc caaagaaaga tcgtagaagg
     2641 cgtagcgaaa aaagtattga gtctaaacgg tgtcagcaac tatcaatccg ttttgaatgc
     2701 tctagagtcg aatatgaaaa cagacatgaa ctttgatgaa atgaaaaaaa ttgctttaga
     2761 ttatcgggag gcatttggta cgattaagca agatcagctg caaggagaag gcttcatgca
     2821 agataatatc tcctatcaaa aagtcagtga tcaagaactt gatcgtgtga aaaaagaatt
     2881 aaaagatcaa atgaatctag agaataaata ggaaagtgga gaaaaaatgg aagagacaat
     2941 cagcttgaaa gaaatttttg aaacattacg caaacacttg gtaataatca tcatctcaat
     3001 gtttgctggc ttggccatct caggaatcgt aacatttttc atcttgacac caaaatacag
     3061 ttcacaagca caattgatcg tgacactgcc acaatcagac actgcaaatg tcaatgatgt
     3121 caatacgaac ctgcaaatga tcaatacgta taaagatatg atcgtcagtg atttagtgct
     3181 caatgaagtc aaagatcgtt tagaaacaga agacaatgtg aaaatgactg ctgaacaaat
     3241 caaagatgcc atctcagtta atcaggcaga aaattcacaa atgttctcga ttcaggcaat
     3301 aagtacgaat gcagtggctg ctcaacagat cgctaacaca acttcacaag ttttccaaga
     3361 gaacgcaaaa gatgtgatga atgttgataa aatctcgatc atttcaggtg cagttgccaa
     3421 tacgacacct gtttcgccaa ataataaatt gaatttaatg actggcttaa tgttaggaat
     3481 gatggtaggt gtaggtctgg cattcttatt ggaattactc gatcggacag tgaaagatga
     3541 aaaatatgtg acagatacat taggattccc aatcttagga acagttccag aaatgtcagc
     3601 gaaagaattg aatgctggta tccgtaaaaa aggaccagct cagacacaac caaaagctac
     3661 aaacgataaa acagcgccat cacgcagaag ccgctcaaga gtctaggagg aaagaaaatg
     3721 gcacgaacac agaaacaaaa agcagcccaa actcatgcag tcagtttggt cacactcacc
     3781 aatccctctt caccaatcgc tgaacaatat cgtacgattc ggacgaatat ccagtttgcc
     3841 tccgcagctg gtcagcaaat caaaacgatt gttgtgactt catcaggacc tggagaaggg
     3901 aaatcgacca ctgcagcaaa cattgccgtt gtttttgcca aatctggtca acgggtgctg
     3961 ctagtcgatg cagacttacg taagcccgtc atctataaaa cgttcaagtt gaacaacgca
     4021 agcggattaa gtactgttct tagttcatca ggaagtgtag cagatgagat ccaacgaaca
     4081 ccagtcgaca atttgtcgat tctgccaagt ggacctaaac caccaaatcc atcagaacta
     4141 ctaagttcgc caagaatgga tcagatttta gcagaagcaa gacaactatt tgatgtagtg
     4201 atcttcgaca tgccgccagt cgttgccgta acagatgcac agatcatgtc ttcaaaaacg
     4261 gatggcacct tattggtcgt acgggaaaat acgtcacgaa aagaatcatt gactaaagca
     4321 aaagaactgt tagatatggt acaagcacga gtcttaggtg tcgtatacaa cggagcagaa
     4381 cacagcaaag atgctggtta ttactacgga aattaaggaa taagaagaga ggagacaaga
     4441 gaaatgatcg atctacattg ccatatccta ccaggaatcg atgacggggc agaaaactta
     4501 gaagcaagta ttgcaatggc tgaaaaagcg attcagcaag gcattacgca tattttatgc
     4561 acaccacacc acaataatgg aaaatacagc aatgaaaaat cacaagtcat ttctctagtc
     4621 gcttcgttac aagccgaatt agagaaaaga cagttgcctt tgacgttatt ggaaggacaa
     4681 gaagtacgta ttaccggaac gctcatcgaa gacatccacc gagatgaaat tttatttaca
     4741 gatttagatg atacatatct tctgatcgaa tttccgacac tggaagtgcc gttatatgca
     4801 gaacggcttt tttttgagtt gttagaacta ggaaagatac cagtgatcgt ccatccagaa
     4861 cgcaacgcgc attttcgaga agacccaaat catttgcttc cgttcctaga tatgggatgt
     4921 ttggcccaac ttactgcgcc aagttatgtt ggtgtatttg gcaaagatat ccagaaaaca
     4981 gccaaaacaa tggtcaaaca taatctcgtg cagatggctg cttctgatgc acacggggtc
     5041 tctaagcgca ccttttactt aaaagagtgc tatgagcaga ttgccaagga gtttggtaag
     5101 gaaaaagtag tacagatgaa acaagtcgca aaggatctga tcaacgggga tgagatcaat
     5161 tatccaatct atgaagaggt aaaaaagaag aagtttggtt tattttagtt ctttattcgt
     5221 agataaaaag gaaatgagga gagagtgaga gatgtataga aaaaactcaa gtggttggtc
     5281 taaacatatt gattttattt tattagactt attttgtgtc cagcttgctt tttatatatc
     5341 atatgtaatg cgacagggtg actggaatcc gtatgtgatt cctatttatc gccacatggc
     5401 aatttttata gaacttgaga atcttacgat tatattttta tttgaggcat ataaaaatgt
     5461 tttaaaaaga ggatattata aagaggctgt agcttctata aagcaatctt ttatgctgtt
     5521 acttgtgtgc agtttatacc ttattacaat gcaggatgga aatgattatt ccagagtttc
     5581 tttatttata atgggggtgg tgtatggatt attaacatat ataattcgta tactttggaa
     5641 agggttgttg catcaccgca tgaaaaacgg tgaagatgta tcgttagtta ttgtaacatc
     5701 tcaaaagatt gcaaaaaatg tagttcgtaa tcttaaagag aagaattatg gcatgtataa
     5761 acttagtggt gtgattgtag tagacagaga tatgtatgga actaccatcg gaggtgtccc
     5821 agtagttgca aatgctgata atgcagcgga atatctttta cagaattggg tagatgaagt
     5881 attcatcaat gttgacgaaa gtgttccata tcccaaagaa ttaatcgcca gatgttcgga
     5941 aatgggactt gctgtacata tgaatcttac aaaagtgtct gatagtacac gtggaaaaca
     6001 atttattggc cgagttggtg attatactgt tattacatct agtattaatg ttatgacgat
     6061 gaggcaagca tttataaaga gaacgattga cattcttgct ggaattattg gatgtattgc
     6121 aacaggtgtt atttttatct tcgtagcacc agttatttac atcaaatcac cagggccgat
     6181 tttctttgca caagaacgta ttggtcaaaa tggtaagaca tttaagatgt ataagttccg
     6241 tagtatgtat atggatgcag aagaacgcaa agcagagttg atgaaagaaa ataagatgag
     6301 taataatctc atgtttaaga tggattttga cccaagaatc attggcaata aaatacttcc
     6361 tgatggaaca aagaagacag gaattggaca atttattcga tctacatcat tggatgagtt
     6421 tccacaattt tataatgtcc taatgggaaa aatgagcctg gtggggttta gaccttgctt
     6481 gaagtcagaa tatgagaagt atgattttca tcatcgtgcg agaattgcga tgaaacctgg
     6541 gattactgga atgtggcagg tgagtggtag aagtgatatc acagacttcg aagaggttgt
     6601 aaaacttgat gcagaatata tcaggaattg gagtatggga ttagatttta gaattttgtt
     6661 taggacagtc aaaactgtgc ttcatagaaa cggttccatg taagataaag atactataga
     6721 aggagtaaaa cgtgatggca agaaaaaaat taaaaatctg tatgctaggt cataaaagaa
     6781 ttccatcaag agaaggtgga attgagatag tggtagaaga attggcaacg cgaatggttg
     6841 cacttgggca tgaagtgact tgctataatc gaagtggtca tcatgttagc ggaaaggaat
     6901 ttgaccagca taaaaataaa gaatataaag gtgtaaaatt aaagactatt tttacattaa
     6961 atataaaagg aattgcagca atgagttctt cagtatttgg tggaattaga gcagcattcg
     7021 gaaaatatga tgttgttcat tttcatgcag aaggaccatg tgctatgttg tggttgccaa
     7081 aactttttgg gaaaagatgt gttgcaacaa ttcatggtct agatcatcaa agagaaaaat
     7141 ggaacaagct tgctagtaca tacatcatgt taggggaaaa gtgtgctgta aaatttgcag
     7201 atgaaataat tgtacttagt gaaagcgtcc aaaattattt tgaagatata tatggaagaa
     7261 aaacaaggtt tataccaaat ggcgttaaaa aaattgaaat aaaatcagca ggattgatta
     7321 cagaaaagta tggattaaca aaagattctt atattttgtt tttggggcga ttggttcccg
     7381 aaaaaggaat tcggtatcta attgaggcat ttaaagatgt tcagacagat aaaaaactta
     7441 taatagccgg aggaagctct gatacagatg aatttgcaaa tgaactaaag gaattagcaa
     7501 aaggtgatga gcgaattata tttactggat ttgtacaagg acaggaatta gaagaattat
     7561 atagtaatgc gtatatctat acacttccaa gtgatttaga aggaatgcca ttaagtttgt
     7621 tagaagcaat gagttatggg aactgttgta tagtttcaaa tatctcagag tgtacggaag
     7681 tagtagaaga caaggctatg atttttaaga aatcggatgt aagtgattta aagataagat
     7741 tagaagaggc atgcaatcaa tcggaaatgg ttaaagtttt gaagaatcaa gctactgagt
     7801 ttatttgtag taaatataat tgggataaaa tcgtacaaga aacattaaat ttgtataggg
     7861 gaaatatata atgattcgaa tattacagtg tgtgaatgat atgcatcgtg cagggttgga
     7921 gacgatgcta atgaactatt ataggaatat agatcgaact aaaattcagt ttgatttttt
     7981 gacacatcgt ccatataaaa gtgattatga tgaggaaata ataagtcttg gaggaaaggt
     8041 ctattatgca ccgagacttt atccacaaaa ttatcctaaa tattttaaat ggatggaaca
     8101 attttttaag gatcatccag aatatgagat tgttcattct catattgatg cgatgagtta
     8161 tttaccttta aaagctgcaa aaaaagctgg gattccgata cggatagcac atagccataa
     8221 tacatcgata gatagagatt ttaaatatct tttaaagcaa tattatcgat gtaagttaac
     8281 gtctgttttg acacatgaat tttcatgtgg aaaagtagca ggtgattttt tgtttcgaaa
     8341 tgataaattc actttgattc caaatgcagt ggatgctaaa aaattttatt atgatgaaag
     8401 tattagaaat gatgtacgaa tgaaattagg aatttcaagt cagacgtttg taatgggaca
     8461 tattggaaga atttcatatt ctaaaaatca tcgttttttg attgaaattt ttaaagaatt
     8521 tcacgctatt aagaataatt ccgtattatt aattattgga actggtgata tggaagaaga
     8581 aataaaaaat tatgcaaaga aaagtggcat agatgatgat attaaatttt taggaaatag
     8641 aaatgatata gaaaaatttt atcaagcatt tgacgtatta atgttgccat cattatttga
     8701 aggggttccg ttagtgggta ttgaagctca atttgcagat ttaccgtgtt tcttttcgga
     8761 gaaagttcct acagaagttg ctttcagtga taagaccaat ttcataagtt taaatcaatc
     8821 ggcaaaagaa tgggcgagag agattgcaaa tgtagatatc tctcatcgag attcagaacg
     8881 ttcgatttta ataaaggcag attataatat tcaaacagca tacaaaatct tagaatcaaa
     8941 atattatgag ttatttgaat taatacaaag aggttaaaga atatgacaca gaaacattca
     9001 agtataaaaa gaattataca tcgcatatcc gaagaattga atgcaacaag acgaattgca
     9061 aagataacat cttggaagga agcatttgcc acgttacaag ctaaaattga tattcaagtc
     9121 atgaatcgta atggatataa tgaaagtaaa aaacagaagg aacatttatt aaaaaaacat
     9181 gaggtaatgc tgaagtatta tgaaaagact ttttcagatt ttttgaaaac atatgattat
     9241 agtcaaaatc aaataattaa tcaatcaaaa tcaccatatt ctaactgcat ttgggtttgt
     9301 tggtggcagg gattggatca atcgccggaa attgtaaaag cttgtgtaaa atcaataaaa
     9361 cgaaatgcag ggaaacatac tgttataatt ttaacagaag ataattatag acaatatgtt
     9421 gatataccaa aatgggtgga agaaaagaaa aacaaaggaa tcattacgag aacaaattat
     9481 tctgatttat taagattatc tttgctagca aaacatggag gtttgtggtt agacgccaca
     9541 ttttattgta caaaaccaat accagatgag tattttgata tcgatatttg gtctattagg
     9601 aggcctgatt atgctcacgc atccgtagca tcaggatatt ttgcgggtta ttcattagca
     9661 tgtaatcaag agtctagatg gatgtttgct ataattagag actttttttt aaattattgg
     9721 aaaaataatg atatgatggt ggattatttg atgatagatt atatgattgt tttagcacag
     9781 agaataaatc atgaaatagc acagacattt gataaaatac ctagtaacaa tccattatgt
     9841 gacgaattga ttaaaaattt aaataatata tatgatgaaa aagtatggaa cttattaaag
     9901 aaagatactt tactgtttaa gttgacatgg aaacaatctt ttaaaaagca aaaagatgat
     9961 aaagttactt tttatgggat gttggtagaa aacaaattat tataaatatt agaggtataa
    10021 aaagttatga aacacaaggt aagtgtgata gtaccaattt acaatgttga aaaatattta
    10081 agaaaatgtg tggatagttt attgagtcag acattaaaag agatagaaat cgtattagtt
    10141 gatgatggtt cgccagatgc ttcgggggaa atagcagatg agtatcaaaa aaaatattct
    10201 aatgtaaaaa caatacatag agaaaatggg gggttaggac cggcaagaaa tacaggaatt
    10261 gagaatgcga caggagaata tgtggctttt cttgattcag atgattgggt agaaagtgat
    10321 atgtatgaaa aattatactt agttgcgtcc aaagaaaatg ctgatatagt agttagtgga
    10381 cattgtgatt ttgcgaatga caaagctatg gtgaaaaagc aacatccttt gagtgggaat
    10441 gtatatgata cgcctaatag tattaaaaat gtacgaaaaa atttatttgg acatagtcca
    10501 aaagatcaag aagtagaggc cttcccgatg tcagtatgta tgtctttgta taataaaaat
    10561 ctgctagata aatatagact aagatttgaa aaaattttat ctgaagatac aatttttaac
    10621 ttgaatgcat atgatgtcgc aaataaaatt gcttttacag aatatacaga ttattgttat
    10681 agaaaagaag aacaagagtc tattacaaaa agtttatctc caaataagca gacggaattt
    10741 aaagaatttt taataactct aaaagctata gcatcgaaag aagatttaga atgtcaaatc
    10801 agagcaaaac gtatggcaat tgattattgt aggctttatg tgggcttagt tgatgatgca
    10861 aatttgggaa ttaaagagaa aagaaagtat ataaaaaaat ttattgaaga tgagggatta
    10921 attcaatgtt ggaagggata tcctacaaat acgctaccat ttcaacaaaa attttttcat
    10981 aaaatgcttg aatgtaagtg ttatacaata ttgttgttgt taagtagaat tagaaagata
    11041 attaaaaata tgaccagaaa aaaattgttt ataatattat aaactaaaag tatatcaaat
    11101 aatagaaatt aacaagttac aaaaaaatat tataaagaat aatattttct aaaaagtaaa
    11161 ctatagtaat gtggattatc accttccaac agtatataaa atgagcgtaa aaacggctat
    11221 ttttgcctta acaacgattt ttttgtatac aaatacgata tgctcattaa ttattggaat
    11281 ttgtagtgct gtttaaatta atgaaggtgt aattatatga gtgatcattt tgaaaaagtt
    11341 tgttgagaag atgtgcttct agtcatgtga tgggagaaaa taatttaact gttagagtga
    11401 atagttaaat tattctggtt atatgaaaaa agtagtttct aaaataatct tgcagggatt
    11461 aacttaaaga aattgagaga atagtttctt tgaaatttct atatacaatg agaagctgaa
    11521 atttactttt atttgatgac tggaaataga aattaaatat attgtattgt tacattaaag
    11581 aaaacttgta tggaaaaatc agtacaatac cattatttaa tgttcttcac ttaaagcgca
    11641 aaaaatatca gtttattaaa ctcgtatata gaacaaatat atgtagacgg ggcgaaacga
    11701 tgagtgtaaa aaaaatgcta tttatggtaa gtacaataat tgtattaaca tcccgatttt
    11761 ggggaattaa tactagttta tctatgcgat attttattat ggcaatatgg gatttatatt
    11821 ttatgttaag ctatttttct tatcgtgaga aaacatataa aaaatacgtt atcaagaata
    11881 acttttggtt gactataatg ccgtttgtga tattttctat ctatactcta ataatatggg
    11941 gaattggttc aaatgcagaa tttgaaaatt ttataaagtt atgttccacg ttgctttatt
    12001 taattcttgc atatggttac gcatgtacag cattttactt atttaaggca gaaggaattg
    12061 attttatttt ttggtcaggt gttatttcat atatgagtgg ttcagttttt tatttaattg
    12121 tatcttatgg tttaaatggt ttaatttctt atataaaaag tcttataaca ggtgaaacaa
    12181 atactgcaaa ttttgctatg gaggtacatg atttaacttt tgcgatgggg gtttttttcc
    12241 tttattacgt attttttgaa aataaaaata caaaaaaaca taagataaaa attatcgtat
    12301 ctttaatttt aattttcatg ggattgaaga gaattgaact gttagcttta agtggtgcaa
    12361 ttctagtgta ttatgtttta ttaaagtggg gaaaaaagat tcaaatccgt gcattagtgt
    12421 gtactatttg tgcaactgtt atttcgttag tgtttattta cattatagat acaggaattt
    12481 tggcattgct tatgtcaaat ttgggcatta cagatgacgg tgcgaggctt tcattttatc
    12541 tatattcatc taaattttat gaacttggtt taggatatgt aggaacagga tggacatggt
    12601 tttcaaaata cttttttaat ctttatttat cgcactttag gttaaatggt ggaagaattg
    12661 cagcaagcct acacagtgat atattaacta tttatataga agttggatgt atagttttta
    12721 ttatatggat gttttggtta ttttttgtga aaccgataag actaactaaa aaatttggat
    12781 tgaaaacagg ggagtgtgta ttattattaa c
//
LOCUS       DACDKM010000081        12733 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20834_2016
            SAMN12640370-rid9891663.denovo.081, whole genome shotgun sequence.
ACCESSION   DACDKM010000081 DACDKM010000000
VERSION     DACDKM010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640370
            Sequence Read Archive: SRR10040891
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12733)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12733)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742835.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742835.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:04:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,012
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 29 of 75
            Pseudo Genes (incomplete)         :: 33 of 75
            Pseudo Genes (internal stop)      :: 19 of 75
            Pseudo Genes (multiple problems)  :: 5 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12733
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640370-rid9891663.denovo.081"
                     /isolate="E20834_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(84..263)
                     /gene="rpmF"
                     /locus_tag="IV334_002048"
     CDS             complement(84..263)
                     /gene="rpmF"
                     /locus_tag="IV334_002048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HAP8944106.1"
                     /translation="MAVLARRTSKAKKAKRRTHYKLTIKGLNECPNCGEMKKSHHVCA
                     NCGYYDGKDVMSKEA"
     gene            complement(321..890)
                     /locus_tag="IV334_002049"
     CDS             complement(321..890)
                     /locus_tag="IV334_002049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HAP8944107.1"
                     /translation="MKWSLLELRKYQETPLTFHETLDLKAALMKRDNLILDVAPVEVE
                     GLVSVDKKGYIVHYTVQTTLTVPSTRSLEPVELPMDFSVDEVFMTEEQFQSRDEQMSA
                     DEILLIEGPLLNLAESIEDNILLAIPMRVLTKEEEAATELPKGNDWEVVSEEEYQRRK
                     MEEAENKIDPRLAKLSELFDSKSKDDDNK"
     gene            1076..1978
                     /locus_tag="IV334_002050"
     CDS             1076..1978
                     /locus_tag="IV334_002050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAP8944108.1"
                     /translation="MNTKDIEYYVQIVKQKNFTKVANYFRVSQPTITYALQRLEKEFD
                     TVLITRDRSHHELIVTPSGKQLFLHAQTILQEISTARAEITQLQKKKLRFGMPPIIGN
                     EYFPKLSSYFIKHELMNQIEITDGGSRDLYGMIRQGRIDLAVLGSTQPIVDQYLETEL
                     LLDKRFMIVVPPTHPLSKQDTVSFKDIADEQFVLLNEHYVHPAAFTKLSKQTHTHPKV
                     VYQSNDLTILKSMVRENVGIGFLAEIAVHPEDHLVCLPLSDEVQPRFLISLAKRTQQI
                     VTPLQEQVVQVIHEFVTAQKNSSS"
     gene            complement(2013..3797)
                     /gene="pyk"
                     /locus_tag="IV334_002051"
     CDS             complement(2013..3797)
                     /gene="pyk"
                     /locus_tag="IV334_002051"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HAP8944109.1"
                     /translation="MKKTKIVSTLGPASNSVEIISQLIESGANVFRFNFSHGDHEEQL
                     SRMTMVREAVKKTGKDVGILLDTKGAEIRTTVQGTTEADFGRAGYIKFEVGDQTRISM
                     DPEHVGSKEKIAVTYPGLFDDVHVGGHILFDDGLIDMQIIEKDEANRELVVEVKNAGM
                     LGSRKGVNAPGVSISLPGITPKDADDIRFGLDNDIDFIAASFVRKAQDVLDIREILEE
                     KDMTHVQIFPKIESQEGIDNIDEIIKVSDGVMIARGDMGVEIPAELVPMVQKRIIKKC
                     NAAGIPVITATQMLESMQENPRPTRAEASDVANAVFDGTDATMLSGESANGDYPVEAV
                     STMARIDQEAENALSELGTFQLNEFDKTDVTETIGLSVARAAKNLGVKTIVAATESGY
                     TAKMISKYRPDADILAVTFDERTKRGLMLNWGVYPTVAEKPSTTDEMFELAAKKAVEL
                     GFASEGDLILITAGVPVGERGTTNVMKIQMIGSKLLEAQGVGQKSVVANAVIASSAEE
                     AVKKAKDGMVLVVPSTDKEYMPAIEKAAAVIVEEGGLTSHAAVVGIAQDIPVIVGAKG
                     ATTTITEGELITVDSRRGIVYRGETTAI"
     gene            complement(3871..4833)
                     /gene="pfkA"
                     /locus_tag="IV334_002052"
     CDS             complement(3871..4833)
                     /gene="pfkA"
                     /locus_tag="IV334_002052"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HAP8944110.1"
                     /translation="MKRIGILTSGGDAPGMNAAVRAVVRKGIYEGLEVYGINYGFAGL
                     VAGDIRRLDVADVGDKIQRGGTFLYSARYPEFATEEGQLKGIEQLKKFGIEGLVVIGG
                     DGSYHGAMALTKRGFPAVGIPGTIDNDIPGTDFTIGFDTAINTVLESIDRIRDTATSH
                     VRTFVIEVMGRNAGDIALWSGVAGGADEIIIPEHDFDMASVAKKIQEGRDRGKKHCLI
                     ILAEGVMGGNEFAEKLSEFGDYHTRVSILGHVVRGGAPSARDRVLASKFGAYAVELLQ
                     EGKGGLCVGIHDNEIVASDIIDTLENNKHKPDLSLYDLNNSLSF"
     gene            complement(5001..8315)
                     /gene="dnaE"
                     /locus_tag="IV334_002053"
     CDS             complement(5001..8315)
                     /gene="dnaE"
                     /locus_tag="IV334_002053"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HAP8944111.1"
                     /translation="MFFPQLYTITSYSLLQSTIRIREYVQEAKKQGYTSLAITDKDVL
                     YGAPEFYRICVEEGVQPIIGLHLSYTYEEKNYGLLAYAKDEIGYQQLMKLSSRKMIQE
                     KLELAEMTETDHLAFVVPANHSVSEAFNKEDNFFGHWQFLLENVFTRNFYIGLTENTP
                     DQLPEWYQYVIKENYSLCALHPIASLQQEELFALEVMKHIKEGSQIPLENLSASLPLT
                     ANVYLLSEQEMIKRFEKAGLSQAVIGAQRLAESISFEFQFHQKLLPHYPVPNGKQAGE
                     YLRELCYSQVERRITDYTKEYQERLDYELSVIHQMGFDDYFLIVWDVMAFAHRNKIVT
                     GAGRGSAAGSLVAYVLEITDVDPLEYDLLFERFLNPERFTMPDIDLDIPDNRREEVLN
                     YVREKYGQYHMAQIATFGTMAAKMVLRDAARVFGLSQSEANRWSKAIPNQLKITLTQA
                     YKTSHQLVELVQASEKNQLLFKTAVILEGLPRHVSTHAAGVVISDKNLLELVPLQRGS
                     DEAFLTQYTMNEVEAIGLLKMDFLGLRNLSIIDFTLKAVKRVEGYEIRLKEIPLNDAK
                     TLLLFQRGETSGVFQFESAGIRNVLRRLDPENIEDIAAVNALYRPGPMQNIDTFIARK
                     KGKEAIRYPDDSLIPILKNTYGVIVYQEQIMQIASTMAGFTLGQSDILRRAVSKKKKD
                     VLDEEKSHFVSGALQQGYSEKTAATVYEYIEKFANYGFNRSHAFAYSFVGFQMAYLKA
                     HHPGAFFTSLMNSVRHNTSKLKEYIAEARKNKLKLSSPSINQSNSSFELVNKEEIRFG
                     LTAIKGIRRDFVEDILNERKQNGPFQSVDQFLIRIDKRWLKLELLQSLTAVGVFDELS
                     PNRKQLMLDLEGKIQNIVYSGGSLDLLGIMALKEEEVADYTLEERLKLEEEYLGIYLS
                     GHPAEGFDRLRLAKQVQLITDIVPNQPVNLLVMIKEVREIRTKKGELMAFVEGTDSSG
                     EISVTVFPTLYRKYRNLFAVDRVIFVQGRSEISKYNQELQLIGESFADPNQLEAQYPS
                     QTCYLRITEEREDPEIMKQLQVIFQKHPGYIPVVMYHEKTQRKVVLSDAYWVDDSKSI
                     KGQLIYLLGEDNVVFK"
     gene            8546..8731
                     /locus_tag="IV334_002054"
     CDS             8546..8731
                     /locus_tag="IV334_002054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjzD family protein"
                     /protein_id="HAP8944112.1"
                     /translation="MRYIVTLFWAVVLGQVVGYIGAALTSGTYDFTLTTIISFIAGVI
                     ILLIGAVAPRKETSAHS"
     gene            complement(8793..9989)
                     /locus_tag="IV334_002055"
     CDS             complement(8793..9989)
                     /locus_tag="IV334_002055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HAP8944113.1"
                     /translation="MEINWRKNLVIAWIGCFFTGASISLVMPFIPVYVEQLGTPKDQI
                     ELFSGLAISVTAFASAVVAPIWGNLADRKGRKLMMIRAAAGMTLTMGSLAFVPNAYWL
                     LIMRFWNGILSGYIPNATAMIASQAPREKSGWALGTLSTGAIAGNLIGPSMGGALAQW
                     FGMENVFLITGALLMITTVLTIFLVKEDFHPIEKKDLISTKEIFSKMDHLSILIGLFI
                     TTLILQIGITTISPILTLYIRELSGSTENILFVSGLIVSIAGVSAVFSSPKLGKLGDK
                     IGNQKVLIGGLILSFCCYLPMAFVTTPLQLGILRFILGFSTGALMPSVNTLISKITPQ
                     EGVSRIYSYNQMFSNFGQVLGPMLGSTVARAYNYSAVFIVTSLFVLSNILLSLFNFRK
                     VLRSRL"
     gene            complement(10159..11595)
                     /locus_tag="IV334_002056"
     CDS             complement(10159..11595)
                     /locus_tag="IV334_002056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HAP8944114.1"
                     /translation="MEKFFGLKKNNTNVSTEIMAGVTTFFAMSYILFVNPTILSASGM
                     PFQAVFLATIIASIIGTLVMGLFANVPYAQAPGMGLNAFFTFTVVFGLGYSWQQALAM
                     VFICGLINIFITVTNIRKMIIRAIPESLQHAIGGGIGIFVAYVGIKNAGFLSFSADQS
                     AISSSVVEGGKATNVTINGGIVPALANFDNAPILLAVIGLVLTAILVVKNVRGAILIG
                     IVATTVLGIFMGVVDLSSIDWQTNSLGNSFKELGTTFGAAFGSEGMQSLFSDSSKIPQ
                     VLMTIIAFSLSDTFDTIGTFIGTGRRTGIFSKEDEIALEDGRGFKTKMDKALFADAIA
                     TSVGAIFGTSNTTTYVESAAGIGAGGRTGLTSVVVAVLFALSSLFSPLIAIVPNQATA
                     PALILVGVMMMASFADIKWLDMEEALPAFFASIFMGLCYSISYGIAAGFIFYTIVKVI
                     KGKYNQVSVALWIVDILFIINFIILATI"
     gene            11888..12697
                     /locus_tag="IV334_002057"
     CDS             11888..12697
                     /locus_tag="IV334_002057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAP8944115.1"
                     /translation="MNKKLIAIDLDGTTLNQDSMITSKTKETLKKAINAGHHVSIATG
                     RPFRMSHQFYQQLELTTPMVNFNGALVHIPNQHWDGERETLINREIAFEILSQKKQLN
                     LDFIAAENRDTFFIDSFDFFDEKIFASSRPGEKNLLSPKNLTTNPTSLLVRTDKRFAG
                     AVSAELTRQFGSYVDVRTWGGPTAILEIVSKGIQKAKGVQEIANYLSIDQKDVIAFGD
                     EHNDLELLDYAGWGVAMANGTDQLKGIANDVTPLSNQEDGLAVYLEKLLKL"
BASE COUNT         3802 a         2674 c         2249 g         4008 t
ORIGIN      
        1 ttttttattt accgactaca aaaaaataaa cctcaaggct ccatggttag ccttgaggtt
       61 ttacattttc taattaaaat gtattaagct tcttttgaca ttacgtcctt accatcatag
      121 taaccacaat ttgcacatac gtgatggctt tttttcattt caccacagtt tggacattcg
      181 tttaaccctt tgattgtcaa tttgtagtgt gtacgacgtt ttgctttttt agcttttgat
      241 gttcttctag ctagtactgc cattttgcta cacctccttg taaagtcttg tatctaaatt
      301 agacacaata tattccaatc ttacttgtta tcgtcgtctt ttgatttaga atcaaaaagt
      361 tcagatagtt ttgcaagacg aggatcaatt ttattctcgg cttcttccat ttttctgcgc
      421 tggtattctt cttcggaaac aacttcccaa tcattgcctt ttggaagttc tgttgctgct
      481 tcctcttctt ttgtcaatac ccgcattgga atcgccaata agatgttgtc ttcaatcgac
      541 tctgctaaat tcaataatgg accttcaatt agcaaaatct catctgcaga catttgttca
      601 tctctacttt ggaattgttc ttctgtcatg aatacttcgt ctacagaaaa atccatgggc
      661 agttcaacag gttctagtga acgtgttgaa ggaactgtca aagtcgtctg cacagtgtaa
      721 tgaacgatat agcctttctt atcaactgag accaaacctt cgacttccac tggcgcaaca
      781 tcaagtatta aattgtctcg cttcatgaga gcggctttca agtcgagcgt ctcatgaaaa
      841 gttaacggtg tctcctggta ttttctcaat tccaataaag accatttcat ttttcatcac
      901 tcctaagcaa caaaagcaat tatacagggc aaaaagcatg ttgtcaatga aattttcttg
      961 acaccttgca gttcataaaa aaacagaaaa atctttatta gaaagcctct gtttgctatc
     1021 atcctcagaa tcatttacaa taatcattat tccattaaag gagcggatga tcttcatgaa
     1081 cacgaaagat atcgaatact atgtccaaat cgtcaaacaa aaaaatttta cgaaagtggc
     1141 taattatttc cgtgtgagcc aacctactat tacatatgca ttacaacgtc ttgaaaaaga
     1201 gtttgacacc gtacttatta caagagatcg atcgcatcac gaattaatcg tcacgccttc
     1261 tggaaagcag ctgtttctcc atgcacaaac cattctccaa gagatttcta ctgcaagagc
     1321 tgaaatcact cagttgcaaa agaaaaagct tcgttttggc atgcctccta ttatcggcaa
     1381 tgaatacttt cctaaactgt caagttactt tatcaagcat gaattgatga atcaaattga
     1441 gattaccgat ggaggctctc gggacttata cgggatgatc cgacaaggga ggatcgattt
     1501 agctgtatta ggctcaacac agccaatcgt ggatcaatat ttagaaacag agttactttt
     1561 ggacaaacgt ttcatgatcg tcgtgccacc tacacatcct ctttccaagc aggatactgt
     1621 ctctttcaaa gatatagcag atgaacagtt tgtactattg aacgaacatt atgtccaccc
     1681 cgcagcattc actaagcttt ctaaacaaac acatactcat ccaaaagttg tttatcaaag
     1741 caatgacttg acgattttaa agagtatggt ccgagaaaat gtgggtatcg gttttttggc
     1801 agagattgct gtccatcctg aagatcattt agtttgcctc cctctttcag atgaagttca
     1861 gcctcgcttc ttgatttcat tggcaaaacg aacacagcag atcgtcacgc ctcttcaaga
     1921 acaagtagtc caagttatcc atgaattcgt tacagcacaa aaaaacagct cttcgtaaaa
     1981 gaagagctgt ttttattttt tattaagcta aattagatag ctgttgtttc gccacggtaa
     2041 acgatgccgc gacgtgaatc aacggtaatc aattcacctt ctgtaattgt tgttgttgcg
     2101 cctttagcac caacgattac aggaatatct tgtgcaattc ctacaactgc agcgtgagaa
     2161 gtcaatccgc cttcttcaac gataacagca gctgcttttt cgattgcagg catatattct
     2221 ttgtcagttg atggtacaac tagaaccatt ccgtcttttg ctttttttac agcttcttca
     2281 gcagatgaag caataactgc atttgcaacg acagattttt gtccaacgcc ttgagcttct
     2341 aataatttag aaccaatcat ttgaattttc atcacgttag ttgttccgcg ttcaccaact
     2401 ggtacaccag cagtgatcaa gattaaatcg ccttctgatg cgaaacctaa ttcaacagct
     2461 tttttagctg ctagttcaaa catttcatca gttgttgatg gtttttcagc aactgttggg
     2521 taaacgcccc agtttaacat caaaccacgt tttgtgcgtt cgtcaaaagt aacagccaag
     2581 atatctgcat ctggacgata ttttgaaatc atttttgcag tgtatcctga ttcagttgca
     2641 gcaacgattg ttttcacgcc tagatttttt gcagcacgag ctacagataa accgattgtt
     2701 tcagttacat ctgttttatc gaattcgttc aattggaatg tacctaattc tgataatgca
     2761 ttttcagctt cttggtcgat acgtgccatt gttgaaacag cttcaactgg gtagtccccg
     2821 tttgcagatt caccagatag cattgttgca tctgtgccat caaatacagc gttagcaacg
     2881 tcagaagctt ccgcacgtgt tggacgtggg ttttcttgca ttgattctaa catttgtgta
     2941 gcagtgataa ctgggatacc agcagcatta cattttttga taatgcgttt ttgaaccatt
     3001 ggtactagtt cagctgggat ttcaacaccc atatctccgc gggcaatcat aacaccgtca
     3061 gaaactttga tgatttcgtc aatgttgtcg ataccttctt gagattcgat tttagggaag
     3121 atttgaacat gagtcatgtc tttttcttct aagatttcgc ggatatctag aacgtcttgt
     3181 gctttacgta caaaacttgc agcgataaag tcgatatcgt tgtctaaacc aaaacgaata
     3241 tcgtcagcat cttttggagt aatacctggc aaactaatag aaacgcctgg tgcatttacc
     3301 cctttacgtg atcctagcat acctgcattt ttaacttcta cgactaattc acggtttgct
     3361 tcgtcttttt caatgatttg catatcgatc aaaccatcat caaataaaat gtgtccgcca
     3421 acgtgaacat cgtcaaataa tcctgggtaa gtaaccgcga ttttttcttt tgaacctaca
     3481 tgttcaggat ccatagagat gcgtgtttga tctcctactt caaatttgat gtatcctgca
     3541 cgaccaaaat cagcttctgt tgtgccttga acagttgtac ggatttcagc tccttttgta
     3601 tctaaaagaa taccgacatc ttttcctgtc tttttaactg cttcacggac catagtcata
     3661 cgtgataatt gttcttcatg gtcaccatga gaaaaattaa agcggaagac atttgcccca
     3721 gattcaatca attgagaaat aatttctact gagttacttg ctggtcctaa tgtactaacg
     3781 attttggttt ttttcattta aaaaactctc ccattcaatt ttgtttattt aaagctgtac
     3841 acagcttaaa tagcttggtt tctttcactc ttaaaatgaa agtgaattat ttagatcata
     3901 tagtgataag tctggtttat gtttgttgtt ttcaagtgta tcaatgatgt cagacgcaac
     3961 gatttcgttg tcatggatac cgacacataa tccacctttt ccttcttgaa gcaattcgac
     4021 agcgtaagca ccgaatttac ttgccaacac gcggtcgcgt gcgcttggtg ctccaccacg
     4081 gacaacgtgt cccaagatag atacacgtgt atgatagtca ccaaattctg aaagtttctc
     4141 agcgaattca tttccgccca ttacaccttc agccaagatg atcaagcaat gtttcttccc
     4201 gcgatcgcgg ccttcttgga tttttttagc aactgaagcc atatcaaaat catgttctgg
     4261 gatgatgatt tcatccgctc cgccagccac acctgaccat aaagcgatgt ctcctgcatt
     4321 gcgtcccatt acttcgataa cgaatgtacg aacatgagaa gtcgctgtgt cacgaatacg
     4381 gtcaattgat tctagtactg tattgattgc tgtatcaaaa ccgattgtga aatcagtccc
     4441 aggaatatca ttatcgattg ttccaggaat acctactgct gggaaaccgc gttttgtcaa
     4501 agccattgcg ccatgataag agccgtctcc tccgataacc actagacctt cgattccgaa
     4561 ttttttcaat tgttcgattc cttttaattg accttcttca gttgcaaatt ctggataacg
     4621 tgcagaatac aagaatgtac caccacgttg gatcttgtca cctacatctg ccacatcaag
     4681 acgacgaata tctccagcaa ctaaaccggc aaaaccataa ttgatcccgt atacttccag
     4741 tccttcgtaa atacctttac gaacaacggc acgaacagct gcattcattc caggtgcgtc
     4801 tccgccactg gtcaaaattc cgatgcgttt cataataaac attcacctca ttaaaatact
     4861 ttatcctaag tatgacttag gtttacaacc atttttgcgt taaccacgac taacattcta
     4921 acattatttt tctcacttgt cttgatttac ggataaaaaa atgaaaaaaa atgaaagcaa
     4981 gtagtatttt ttaccgtaag ttatttgaaa accacattat cttcgccaag cagataaata
     5041 agttgcccct ttatactctt gctgtcatcc acccaataag catctgacag aactactttt
     5101 cgttgcgttt tttcgtgata cataactact ggtatatatc ctgggtgttt ctgaaaaatc
     5161 acttgtaatt gtttcattat ctcgggatct tcccgttctt ctgtaatacg caaatagcaa
     5221 gtttgtgaag gatactgggc ttctaactgg ttaggatcag caaagctttc tccaattagt
     5281 tgcagttcct gattatattt gctgatttcg ctccgccctt gaacaaagat cactcgatca
     5341 acggcaaaca aattacgata tttacgataa agagttggaa aaactgtgac agaaatttct
     5401 ccactactgt cggttccttc aacaaaggcc atcagttctc ctttttttgt ccgaatttct
     5461 ctaacttcct tgatcatcac taataaattg actggttgat tagggacgat atctgtaatc
     5521 agctgaactt gtttagctaa acgaagacga tcaaaccctt ctgcaggatg acctgacaaa
     5581 taaataccta agtactcctc ttccagcttc aagcgttctt ctagtgtata gtcagcgact
     5641 tcttcttctt ttaatgccat gattcccagc aagtcaagac ttccaccact gtaaacgata
     5701 ttttggattt ttccttcgag atcaagcatc aattgtttcc tgttaggaga taattcatca
     5761 aaaactccta ctgctgtcag tgattgcagt aactctaatt tcagccaacg cttatcgatc
     5821 cgtatgagga attgatccac tgactggaac gggccatttt gttttctttc gtttaatata
     5881 tcttctacaa aatcacgccg aatacctttt atcgctgtca gtccgaatct gatttcttcc
     5941 ttattgacca actcaaaact ggaattactt tgattaatgg acggcgagga aagtttgagt
     6001 ttatttttac gtgcttctgc aatatattct ttcaattttg atgtgttatg ccgaacagaa
     6061 ttcatcaggc tggtaaagaa agcacctgga tgatgggctt ttagatacgc catttggaat
     6121 cccacgaatg aataagcaaa tgcatgtgac cgattgaatc catagtttgc aaatttttcg
     6181 atatattcat atacggtcgc tgctgttttt tccgaatatc cctgttgtaa tgctcctgat
     6241 acaaaatgcg acttttcttc atctaatacg tcttttttct ttttactcac tgcccgtcgc
     6301 agaatatctg actgccctaa tgtaaatcca gccatagtcg aggcgatctg catgatttgt
     6361 tcctgataga cgatcactcc atatgtgttt ttcaaaatcg ggatcagtga atcatctggg
     6421 tagcgaatgg cttcttttcc tttcttcctg gcaatgaagg tatctatatt ttgcataggg
     6481 cctggtcgat acaatgcatt gactgcggca atatcttcga tgttttctgg atctaatctt
     6541 cgtaaaacgt tccggatacc agcagattca aattgaaata ctcctgaggt ttcgcctcgt
     6601 tgaaacaaga gcagcgtttt tgcatcattt agcgggatct cttttaatcg gatctcatac
     6661 ccttctactc gcttaacagc tttcaaagta aaatcaataa tcgataagtt gcgtagtcct
     6721 aaaaagtcca ttttcaaaag accgattgct tctacctcat tcatcgtata ctgggtcaga
     6781 aaagcttcat cagaaccgcg ttggagcgga actagttcaa gtagattctt atcgctgata
     6841 actacacctg ctgcgtgagt cgaaacatgt cttggcaatc cttctaaaat cactgcagtt
     6901 ttgaataaaa gttggttttt ttcgcttgcc tgaactaatt ctactaattg atgcgaagtt
     6961 ttataagctt gggtcaatgt gattttcaat tgattgggta ttgcttttga ccagcggttt
     7021 gcttcacttt gcgataaacc aaacactcta gctgcatctc taagaaccat ttttgctgcc
     7081 attgtgccaa atgttgcgat ttgtgccata tgatattgtc catacttttc tcgaacataa
     7141 ttcaatactt cttcacgtcg attgtcagga atatccaaat cgatatctgg catcgtaaag
     7201 cgttcggggt ttaaaaagcg ttcgaacagc aaatcatatt ctaatggatc cacatctgtg
     7261 atttctaaca cataggctac taatgaaccc gcagcagaac cacgacctgc accggtaacg
     7321 attttgtttc tatgagcaaa agccattaca tcccatacga tcaagaaata atcatcgaat
     7381 cccatttgat ggatgacaga taactcgtag tccaaacgtt cttggtattc ctttgtatag
     7441 tcagtgattc ggcgttccac ttgcgaatag cataactctc ttaaatattc tccagcttgt
     7501 tttccattag gaactggata atgcggaagt aatttctgat ggaactggaa ttcaaatgaa
     7561 atcgattcag ctaaacgctg ggcgcctatt acagcttggg aaagacccgc tttttcgaat
     7621 cgtttgatca tttcttgttc tgaaagaaga tagacatttg ctgttagtgg taaagatgca
     7681 gataaatttt ctaaaggtat ttggcttccc tctttgatat gtttcatcac ttcaagtgca
     7741 aaaagttctt cttgttgtaa actagcgatg ggatgtaatg cacaaagcga ataattttct
     7801 tttatcacat actgatacca ttcaggcaat tgatcaggcg tgttttctgt caagccgata
     7861 taaaaatttc tagtaaaaac attttcaagt aaaaactgcc aatgaccgaa aaagttatcc
     7921 tctttattga aagcctcaga aacagaatga tttgccggaa caacaaacgc tagatggtcg
     7981 gtttctgtca tttctgcaag ttccaatttt tcttggatca tttttcggct ggaaagtttc
     8041 atcagctgct gataacctat ttcatctttt gcgtacgcaa gcaagccata atttttttct
     8101 tcataagtat aactaagatg taatccaatg attggctgta ctccttcttc tacacaaata
     8161 cgatagaatt caggtgcacc gtataagaca tctttatccg ttatggctaa tgaagtgtag
     8221 ccttgttttt ttgcttcttg tacatattct cgaatacgta tcgtactttg taaaagagaa
     8281 taagaagtaa ttgtataaag ttgtgggaaa aacatcttgc attccgcctt ttttttcgtt
     8341 aaaattcact agcgttaaaa ggtttgatcc ttttactttc attaagatag caggcgctga
     8401 agttgattat tccacttcat ttgcttgctt gtaatctgta ggaatgagtt ctttcgcaag
     8461 aactcaaatt ttattgagat ttggtctcgt ttctttacat tcaaagaaaa tctggtaaaa
     8521 ttaagataga aaagaggtgt tctttatgcg atatatcgtt acattattct gggctgtcgt
     8581 tctaggtcaa gtagtcggtt atattggtgc tgcattaaca agcggaacat atgattttac
     8641 tttgacaaca atcatttcat tcattgcggg tgtcattatc cttttgattg gtgcagttgc
     8701 gccaagaaaa gaaacctctg cccattcgta aaaaaacagc caagagcttg agatttctca
     8761 agctcttttt tattttctac tcctcaccac cattataatc tgctgcgaag aactttacga
     8821 aagttgaata aagaaagaag aatatttgac agaacaaaca aacttgtcac aataaaaaca
     8881 gcactgtaat tataagcacg cgcgactgtc gatcctagca tgggacctaa aacttgtcca
     8941 aagttgctaa acatctgatt gtagctatag attcggctga ctccttcttg aggcgtaatc
     9001 ttactgatca gtgtattgac agaaggcatc aaagcgccag ttgaaaagcc taaaataaag
     9061 cgaagtatac ctaattggag cggtgtagtg acgaatgcca taggtaaata acagcaaaat
     9121 gataaaatga gtcctccaat tagaactttt tgattcccaa tcttatcccc tagttttcct
     9181 agtttcggtg aagaaaagac agcggaaaca ccagctatcg aaacaatcaa gccgctgaca
     9241 aaaaggatat tttctgtact tccgctcaat tcacgaatat ataaggtcaa aatagggctg
     9301 atcgtagtga ttcctatctg taaaatcaag gtcgtaatga agagaccgat caaaatagag
     9361 agatgatcca tttttgaaaa aatttctttg gtgcttatca gatctttttt ctcgattgga
     9421 tggaaatctt ctttgactaa gaaaatggtc aataccgttg tgatcatcaa caaagcccct
     9481 gtgatcaaga atacattttc cataccaaac cactgagcca gtgcaccgcc cattgaaggt
     9541 ccgatcagat ttccagcaat cgctccggta gacaatgttc ctaacgccca tccgctcttt
     9601 tctctaggtg cctgtgaagc aatcatcgct gtggcatttg gaatatatcc agacaaaatc
     9661 ccattccaaa accgcatgat caaaagccaa tacgcgtttg gtacaaaagc caaagatccc
     9721 attgttaatg tcatccctgc tgctgcacgg atcatcatga gttttcggcc ttttcgatca
     9781 gctagatttc cccagattgg tgcaaccacg gcagatgcaa aagcagtcac agagattgct
     9841 agtcccgaaa acaattcgat ttgatctttt ggtgttccta gttgctcaac ataaactggt
     9901 atgaatggca taactaaact aatacttgca ccagtgaaaa aacagccaat ccacgcaatc
     9961 actaaatttt ttctccaatt aatctccatc tatcatccct ctttcgtctc ttttatcata
    10021 cctattttcg aataaacaag caacgatctc gccagtaaaa agagaaaaat cccgcaaagg
    10081 tcattttata tgtaacgttc tttttatgta aaacaaaaag agatcagaac aaatactgtt
    10141 ccaatccctt ctctttagtt aaattgttgc tagaatgatg aagttgataa tgaataaaat
    10201 atcaacgatc cacaaagcaa cagaaacttg attgtatttt cctttgatta ctttcacaat
    10261 tgtatagaaa ataaatcctg ctgcaatccc ataggaaatg ctatagcaca atcccataaa
    10321 gatcgatgca aagaaggccg gtaaagcttc ttccatatct agccatttga tgtctgcaaa
    10381 agaagccatc atcattactc caactaaaat caatgctggc gcagttgctt gatttggtac
    10441 aatggcgatc agtggagaga ataaactact taatgcgaat agcactgcca ctactactga
    10501 agtcaaacca gtacgtccac cggccccgat cccagctgca gattcgacat aagttgttgt
    10561 attcgatgta ccaaagatag cccccacaga agtagcgatc gcatcggcaa atagtgcttt
    10621 atccattttt gttttgaacc cacgaccatc ttccaacgcg atctcgtctt cttttgagaa
    10681 aatacctgtg cggcgtcctg taccgataaa tgttccgatc gtatcgaaag tatcagataa
    10741 actgaatgcg atgatcgtca tcaatacttg aggaatcttg gaagaatcac tgaataaaga
    10801 ttgcatgcct tcagaaccaa atgctgcacc gaaagttgtt cctaattctt taaatgaatt
    10861 acctaacgaa ttcgtttgcc aatcaatgga acttaaatca acaacaccca taaaaatacc
    10921 aaggactgtg gtcgctacga taccgataag aatagcccca cggacatttt ttacaactaa
    10981 aatggctgtc agtaccaaac caatcacagc caataagata ggagcgttgt caaagttggc
    11041 taacgcaggg acgattccac catttatcgt aacatttgtc gcttttccgc cttcaacgac
    11101 agagctcgaa atagctgact ggtctgctga aaaagataag aatcctgcat ttttgattcc
    11161 gacatacgca acgaaaatac cgatcccgcc accaatcgca tgttgaagac tttcgggaat
    11221 cgcccgaata atcatttttc gaatatttgt gacagtaata aaaatattga ttaatccgca
    11281 aataaacacc attgccaagg cttgttgcca agaataacca agcccaaaaa cgaccgtaaa
    11341 agtgaaaaat gcgttcaatc ccataccagg tgcctgagca tatggtacat ttgcgaacaa
    11401 tcccattacc agcgtaccaa taatcgatgc aatgattgtc gctagaaata ctgcttggaa
    11461 gggcatacct gatgctgaca aaatcgtcgg atttacgaat aagatataac tcatggcaaa
    11521 gaatgtcgtt actcccgcca ttatttcagt tgatacattt gtattgtttt tctttaaacc
    11581 gaaaaacttt tccataaaaa gttccactcc ctgaattttt ttctgatgga aaactataat
    11641 aataaaaaat aagtaaaata gggcaaatcc atcgaacaat ttcaaattat atagacgttt
    11701 cattcgcttt tcaacgtttt tttaacaaaa aaagcgaaaa taatacttaa cgttcgtgtt
    11761 tagcttatgt ttagctattt atatagccat aactgtagtt tttgttagga atagctgatt
    11821 gctcttgtta attacctaaa atatgctaga ctaaaacaaa tgctagttga ggaaggaagg
    11881 acccttattg aataaaaaat taattgccat cgatctggac ggaactaccc tcaatcaaga
    11941 ttcgatgatt acatctaaaa caaaagagac tctaaaaaaa gcaatcaatg ccggtcatca
    12001 tgtcagtatt gctacaggac gcccattccg aatgagccat cagttttacc aacaattaga
    12061 attaacaaca ccgatggtta attttaacgg cgcattagta cacatcccta atcagcactg
    12121 ggatggtgaa agagaaacgt tgatcaatcg tgaaatcgct tttgaaatct tatcgcaaaa
    12181 aaagcagcta aatttagatt ttatcgctgc tgaaaatcgt gatacttttt ttattgacag
    12241 ttttgatttc tttgatgaaa aaatttttgc ttcttctcgt ccaggagaga agaatttgct
    12301 ttctcctaaa aatttgacga caaatcctac atctttgctt gtccgaacag acaaacgttt
    12361 tgcaggagct gtatctgctg aattaacacg tcaatttggc agttatgtcg atgttcggac
    12421 atggggtgga cctactgcca tcttagagat tgtttcaaaa ggaatccaaa aagcaaaagg
    12481 cgtgcaagag attgctaact acctgtctat cgatcaaaaa gacgtcattg cctttggtga
    12541 cgagcataat gatttggaat tgttggatta tgcaggttgg ggagtagcta tggctaacgg
    12601 tactgaccaa ctcaaaggca ttgcaaatga tgtcactcct ttgagcaatc aggaagacgg
    12661 tttagctgtt tatctggaaa aactacttaa actataatag agaattagat tccacaaaaa
    12721 agaggttgtg aca
//
LOCUS       DACDKM010000082        12208 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20834_2016
            SAMN12640370-rid9891663.denovo.082, whole genome shotgun sequence.
ACCESSION   DACDKM010000082 DACDKM010000000
VERSION     DACDKM010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640370
            Sequence Read Archive: SRR10040891
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12208)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12208)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742835.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742835.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:04:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,012
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 29 of 75
            Pseudo Genes (incomplete)         :: 33 of 75
            Pseudo Genes (internal stop)      :: 19 of 75
            Pseudo Genes (multiple problems)  :: 5 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12208
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640370-rid9891663.denovo.082"
                     /isolate="E20834_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            11..733
                     /locus_tag="IV334_002058"
     CDS             11..733
                     /locus_tag="IV334_002058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HAP8944116.1"
                     /translation="MLLLTLVVGYFSIEFIHGFSSAKQSSTVKKVEPKSIPTTLNVAL
                     IGSDARSKDENGRSDSLLVAQYNQKTQQAKLISIMRDSYVDIPGYGMNKINAAYSYGG
                     VDLLNQTLKENFKFEAPYYASITFQDFIDCVNELFPGGVKIDAEKSLDLDGVYINKGE
                     QVMDGNTLLQYARFREDKEGDFGRIRRQQQVIKAISQQLKDVTSIFKLPKAVGKLLGS
                     IQTNLPESVLLDCGMDFLKDIC"
     gene            791..2830
                     /locus_tag="IV334_002059"
     CDS             791..2830
                     /locus_tag="IV334_002059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HAP8944117.1"
                     /translation="MKRSDKHGKNRTGAYIAGAVILIAAAGGGYFYYQHYQETQAVEA
                     GEKTVEQFVQALNKGDYNKAAEMTSKKAANKSALSEKEILDKYQNIYGAADVKGLQIS
                     NLKVDKKDDSTYSFSYKAKMNTSLGELKDLSYKGTLDRNDGQTTINWQPNLVFPEMEG
                     NDKVSLTTQEAARGNIIDRNGEPLATTGKLKQLGVVPSKLGDGGEKTANIKAIASSFD
                     LTEDAINQAISQSWVQPDYFVPLKIIDGATPELPAGATIQEVDGRYYPLGEAAAQLIG
                     YVGDITAEDIDKNPELSSNGKIGRSGLEMAFDKDLRGTTGGKLSITDADGVEKKVLIE
                     HEVQNGKDIKLTIDAKAQKTAFDSLGGKAGSTVATTPKTGDLLALASSPSYDPNKMTN
                     GISQEDYKAYEENPEHPFISRFATGYAPGSTFKIITAAIGLDNGTIDPNEVLTINGLK
                     WQKDSSWGSFQTTRVSSDVSQVDLKTALIYSDNIYAAQETLKMGEKKFRTGLDKFIFG
                     EDLDLPISMNPAQISNEDSFNSDILLADTGYGQGELLINPIQQAAMYSVFTNNGTLVY
                     PKLIADKETKDKKNVISETAVQTIVPDLREVVQDVNGTAHSLSALGIPLAAKTGTAEI
                     KEKQDVKGQENSFLFAFNLDNQGYMMVSMLENKEDDDSATKRASELLQYLNQNYQ"
     gene            complement(2939..4090)
                     /locus_tag="IV334_002060"
     CDS             complement(2939..4090)
                     /locus_tag="IV334_002060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton antiporter"
                     /protein_id="HAP8944118.1"
                     /translation="MEFIGILCLILISTTIGSHISRRLGIPAVIGQLLVGVLLGQAGL
                     NWVKPDILVHDFSEIGVILLMFLAGLESDLSLLKKYFRPGMFVALLGIIFPVVFGWLT
                     GKAFQVSGNEAIFFGIILAATSVSISVEVLKELNVVNTKEGSTILGASVVDDILVVLV
                     LSFSLSFLTGEASSNASLPLVLIEQLIYFVFIFLLVKWIAPFLLSFAEKVYANSSIII
                     VSLVICLGMSYVADLVGLSSVIGAFFAGIAVSQTKVKEEVYHNVEALGYAVFIPVFFV
                     SVGLEVDFSDFSEQLLFISILTLVAILTKLLGGYFGAQIAGFSQHSSLMVGAGMISRG
                     EMALIILQIGQQSQLIEPQYYSPLVIVILLSTLISPLVLKYFTKKVYEV"
     gene            complement(4244..4774)
                     /locus_tag="IV334_002061"
     CDS             complement(4244..4774)
                     /locus_tag="IV334_002061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8944119.1"
                     /translation="MFETIKQNIQKYALLRAAVYIISGIAIVLNPSAVFHFIGYLITA
                     YLVLLGLLNLFEAYRFKRKTGSWSIGLFSGLFFLILALIVYAFAPAIVSILPILLGLA
                     VIINSLFQLFFSMNTKAKSWSVYSSILLLIGGFVLLFNPFKSLMLLFQIFGFILIFMG
                     IQEIINFIRIRKMTIR"
     gene            complement(4873..5223)
                     /locus_tag="IV334_002062"
     CDS             complement(4873..5223)
                     /locus_tag="IV334_002062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2200 domain-containing protein"
                     /protein_id="HAP8944120.1"
                     /translation="MAQNRIFTTSFASVYPMYVAKAERKDRIKEEVDEIICWLTGYSQ
                     TELEYQLQKQVTFEQFFAEAPVPNPSRSLITGVVCGVRVEDIEDNLMKEIRYLDKMID
                     ELAKGKKMEKILRS"
     gene            complement(5380..6909)
                     /gene="ahpF"
                     /locus_tag="IV334_002063"
     CDS             complement(5380..6909)
                     /gene="ahpF"
                     /locus_tag="IV334_002063"
                     /EC_number="1.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit F"
                     /protein_id="HAP8944121.1"
                     /translation="MLDKETKQNLEQYLALIESPIVFSVSLDNSENSQKLAEFTKEIA
                     EMSPKISWEKRDSMRTPSFSINPVGKESGIVFAGIPLGHEFSSFLLAMLQISGRAPKI
                     SESLSLKIKKITQTLHFDSYVSLTCHNCPDVVQALNILAVLNPNITHTMIEGGMFQKE
                     IDDKKIMAVPTVFLNGEEFSSGRMTIEQIVEKITGPLIEEELFEKEPYDVLVIGGGPA
                     ASSAAIYAARKGIRTGLVADSFGGQVVETLGIENMIGTSYTEGPKLMQQVESHVRSYP
                     IDIMMNQQAVSLNKEQHFINIGLANGISLKAKTAIIATGAHWRSINVPGEKEFKNKGI
                     AYCPHCDGPLFKGKEIAVIGGGNSGVEAAIDLAGLAKHVYVLEFLPELKADQILQDKL
                     VSLANVTVIKHAATKEISGTNQVESLSYIDRRTMEMHTLAVSGVFILVGLLPNTDWLD
                     GTIEMNPRNEIVTEKNGATNIPGVFAAGDCTDSPYKQIIVSMGSGATAALGAFDYLMR
                     T"
     gene            complement(6919..7482)
                     /gene="ahpC"
                     /locus_tag="IV334_002064"
     CDS             complement(6919..7482)
                     /gene="ahpC"
                     /locus_tag="IV334_002064"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003756453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HAP8944122.1"
                     /translation="MSLIGKKIEEFNAHAYHQGEFLEVSEKDLMGNWSILCFYPADFT
                     FVCPTELEDLQEQYSTLKSLGVEVFSCSTDTHFTHKAWHDTSDAIGKIEYVMIGDPSH
                     QISRIFDVLDEEQGLAQRGTFIIDPDGIVQAMEINADGIGRDASALIDKIRAAQYVRT
                     HPGEVCPAKWKESGETLKPSFDLVGKI"
     gene            complement(7626..8288)
                     /locus_tag="IV334_002065"
     CDS             complement(7626..8288)
                     /locus_tag="IV334_002065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAP8944123.1"
                     /translation="MKSSLQSQAYQAIRKQIIYADLEPGRKISEKALEEHLAIGRTPI
                     REALIQLRQQELVYTIPQSGTYVSKINLVSAENARFVREQLEEQIMIECCSKLDTQTR
                     IVLETILEEQEKAVKINDERAFFRADNLFHEACFEIAGRKEVWNWLDDHNTHLERFRW
                     LRVTIQGLPWETIMEQHYDLLKALVDKKPEEARFQTGLHLHMMLKEQETVTQRYPEYF
                     QQ"
     gene            8472..10118
                     /locus_tag="IV334_002066"
     CDS             8472..10118
                     /locus_tag="IV334_002066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HAP8944124.1"
                     /translation="MIDLNNWRNFIPELSQKEVEIPHFDRASLRENGQKNPVWIHFGG
                     GNLYRGFHAEIAQRLADLGELSTGVIVCETFDEEIIERAYQDFDDLILEVVMPSSGEF
                     VKRILAATADSLYCHPDCSEHFDKMIRHFENPSLQLVTFAITEKGYRLKDTNNKYIGP
                     IAVDIKNGPKKPSHTMSILAALLYKRFLSGAWPIALVSTDNFSQNGSQLRTSLLTIAK
                     EWQKEGYVQNTFIQYLSNEEIVAFPWTMIDRITPNPSLSVSKMLAKEGFSNMSIFQTA
                     KGNNIAPFVNTEDIHYLIMEDHFPNGRPKFEKAGVFLTDRATVDKADTMKVTTCLNPL
                     HTSLAIMGCLLGYRSIAEEMKDPDLSLLVSEIGYNEGLPVVESPGIIDPKIFLEEVIT
                     VRFSNPNIPDMPQRIATDTSQKVAIRYGETIKKYMEIEVKDPQTLVFIPLTLAAWLRY
                     LIGIDDQGKPFIPSPDPLLTDFQHKLSDIQLGTQDTSHIHEKMAPILSNKEIFGVDLY
                     QAELGRKIEEMFIKMVAGPNAVRKTLQDYLQEHGGNLSWK"
     gene            10109..10237
                     /locus_tag="IV334_002067"
     CDS             10109..10237
                     /locus_tag="IV334_002067"
                     /inference="COORDINATES: protein motif:HMM:NF015727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8944125.1"
                     /translation="MEMTFRWYGSQEEKITLDEIRQIPAVKGIVGTLMDHRKKRLP"
     gene            10204..11268
                     /gene="uxuA"
                     /locus_tag="IV334_002068"
     CDS             10204..11268
                     /gene="uxuA"
                     /locus_tag="IV334_002068"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HAP8944126.1"
                     /translation="MDGSQEEKITLDEIRQIPAVKGIVGTLMDIPVGEVWPRERIKEL
                     KEEIESRGLTLKVIESVNVHDAIKIGLPSREQYIENYKQTIRNLAEYGIEVICYNFMP
                     VFDWVKSDLDYRLEDGSSTLAFISDDIPDDPREIVARIEHSSNEFELPGWEPERLAHI
                     KSLLEAYASVDEEKLRENFAYFLQSIIPTCEEVGVKMAVHPDDPPYSIFGLPRIVKNR
                     EDLDWLCRIVDSPYNGITLCTGSIAEDPENDVYGILAEFCQRDRIPFAHVRNIKFLAN
                     RDFYEAPHKSEYGSLDMYRILKTMYDNGFNEYIRPDHGRMIWNETGRPGYGLYDRALG
                     AVYLNGLWEAIEKGNKSKDN"
     gene            complement(11376..>12208)
                     /locus_tag="IV334_002069"
     CDS             complement(11376..>12208)
                     /locus_tag="IV334_002069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="M protein trans-acting positive regulator"
                     /protein_id="HAP8944127.1"
                     /translation="TEYEYIDFFIDPSVDILRPNLPICREQRILVNILARLSITKIES
                     EETSLEQYKLLKKANVPHVLYSREMVESFCTVFDLFIPEKEKILYSLAFARNKLYNGF
                     LNFNQPNTPLPTFMLYQEHSVLKEVQTAIEQFYMDFREQNQHLLPVILEKENVQWMVE
                     DLVHLYDRYKKQPRIVIGVNYTRDFYISKDLIVKIEQIFSNESIKIQNDFMENCNIVI
                     SDCPLKQLPSHIKKIYILKGIITPEDWKKVIMRISEYIFELKEEALMDQSKKENQVTS
                     "
BASE COUNT         4081 a         2263 c         2292 g         3572 t
ORIGIN      
        1 attatctatt ttgttactgc ttacattagt agttggctat ttttcgattg agtttatcca
       61 tggattttcg tctgcaaaac agagttcaac tgtgaaaaag gtagagccga aaagtatccc
      121 taccacacta aatgtggctt tgattggttc agatgctcgt tcgaaagacg aaaacggtcg
      181 ctcggattca cttctggttg cacaatacaa ccagaaaaca caacaagcaa aactgatctc
      241 tatcatgaga gattcatatg tcgatattcc aggttacgga atgaataaga taaatgcagc
      301 ttactcttat gggggagttg atttattaaa tcaaacatta aaggaaaatt tcaaatttga
      361 agctccctat tatgcaagta tcacatttca agattttatc gattgcgtta atgaactgtt
      421 tcctggtgga gtcaagatcg atgcagaaaa atctctagat ttagatggcg tatatataaa
      481 caaaggagag caagtgatgg atggtaatac tttactccag tatgctcgat ttcgggaaga
      541 caaagaagga gacttcggga ggatcagaag gcagcagcaa gtgatcaaag ctatctctca
      601 gcagctgaaa gacgtaactt caatattcaa gctacccaaa gcggttggga aattacttgg
      661 tagtatccaa acgaaccttc cagaaagtgt gcttctggat tgtggtatgg atttcctcaa
      721 agacatatgt tagaataaac aggtataaat agtgaaataa aggaatgaca agcaagagaa
      781 ggaggaaaaa atgaaaagaa gtgacaagca cggcaaaaat cgaacaggcg cttatattgc
      841 cggcgcagtg atcttaatag cagctgcggg tggcggttat ttttactacc agcactatca
      901 agaaacccaa gcagtagaag ctggagaaaa gacagttgag caatttgtcc aagctttgaa
      961 caagggagat tataacaaag ctgcagaaat gacatcaaaa aaggcggcaa ataaaagtgc
     1021 attatctgaa aaagagatct tagataaata ccagaatata tacggtgctg ctgatgtcaa
     1081 aggacttcag atatcgaatc taaaagtaga taaaaaagac gattctactt acagtttttc
     1141 atataaagca aagatgaata cctcattagg tgaattgaaa gatctttctt ataaaggaac
     1201 attagacaga aatgatggtc aaaccacgat caactggcaa cccaacttgg tttttccaga
     1261 aatggaagga aatgacaaag taagcctgac cacacaagaa gcagcaagag ggaatattat
     1321 agatcgaaat ggtgaaccat tagcaacaac aggcaaacta aaacaattag gagtcgttcc
     1381 aagtaaactt ggagatggag gcgaaaaaac agccaatatc aaagcgattg cttcctcctt
     1441 cgacttaaca gaagatgcta tcaatcaggc gatttcacaa agctgggtac aacccgatta
     1501 ctttgtccca ttgaaaatca ttgatggagc aacgccagaa cttccagctg gagctaccat
     1561 tcaagaagta gacggcagat attatccttt gggtgaagca gctgctcagc tgattggtta
     1621 cgtgggagat atcacagcag aagatattga taagaatcca gaattaagca gtaatggaaa
     1681 aatcggtcgc tcaggtttgg agatggcttt tgataaggat cttcgtggga caacaggtgg
     1741 gaaattaagc attacagatg cagacggtgt agagaaaaaa gttctgatcg aacatgaagt
     1801 tcaaaatggc aaagatatca aattgacaat cgatgcgaag gcacaaaaaa cagcttttga
     1861 cagtctagga ggaaaagctg gatcaactgt tgcgacaacg ccaaaaaccg gtgatcttct
     1921 tgcgcttgct agctctccaa gctatgatcc aaacaaaatg acaaacggga tctcacaaga
     1981 agattacaaa gcttatgaag aaaatcctga acatccattc atcagccgat ttgcgacagg
     2041 ttatgctcct ggatctacgt ttaaaataat tacagcagca atcggtctcg acaacggcac
     2101 tatcgatcca aatgaagtgt tgacgatcaa cgggcttaaa tggcaaaaag acagttcttg
     2161 gggatctttt caaacaacgc gtgtcagtag tgatgtatca caagtagact taaaaactgc
     2221 tttgatctat tccgataata tatatgcggc acaagaaacg ttgaaaatgg gtgagaaaaa
     2281 atttcgtaca ggcttagata aattcatttt tggtgaagac cttgatttgc caattagtat
     2341 gaatccagca caaatttcta atgaagatag ctttaactca gatatcttgc tagctgatac
     2401 tggatatgga cagggcgaac ttctaattaa tcctatccag caagcagcaa tgtattctgt
     2461 ttttaccaac aatggcacac ttgtctatcc taaattgatt gcagataaag agacaaaaga
     2521 taagaagaat gtcatcagcg aaacagcagt acaaacgatc gtgccagatc tgagagaagt
     2581 tgtgcaagat gtaaatggta cagcacattc tctttctgct ttagggattc cgttggcagc
     2641 gaaaactggt acagcggaaa tcaaagaaaa acaggatgta aaggggcaag agaacagttt
     2701 cttgtttgct ttcaatcttg ataaccaagg ttatatgatg gtcagcatgt tggaaaataa
     2761 agaagatgat gattcagcaa ccaaacgagc atcggaacta ttgcaatacc tcaaccaaaa
     2821 ttatcaataa aaaattgctt tttaagtaca gtaaaaggac tgtggcgtta tcgtcacagt
     2881 ccttttttca aataaacggt tttcacgatg tctctttcac aacttctttt tgatttactc
     2941 acacttcata aacttttttt gtaaaatatt tgagaacaag tggagaaata agtgtactta
     3001 ataaaatcac gataactaaa ggtgaatagt actgtggttc gatcaattga ctttgctgtc
     3061 caatttgtaa aatgatcaaa gccatttctc cacgcgaaat cataccagca ccgaccatca
     3121 atgaactatg ttgagaaaaa ccggcaatct gagcaccaaa ataacctcca agcaatttgg
     3181 tcaaaatcgc tacaagcgtc aatatagaaa taaagagtag ttgttcacta aagtcagaaa
     3241 aatcaacttc taatcctaca ctgacaaaaa atactggtat aaatactgca tatcctaaag
     3301 cctctacatt gtggtatact tcttctttaa cctttgtctg actgacagct atcccagcaa
     3361 aaaaagcacc aatgacagag ctaagcccaa caagatccgc tacgtaagac attcctaaac
     3421 aaataactag cgatacgatg ataatagaag aatttgcgta cactttttct gcaaatgaaa
     3481 gtaggaaagg cgcaatccat ttgactaata agaaaataaa aacaaaataa attagctgtt
     3541 caatcaaaac aagcggtaaa gaagcattgc tagaagcttc ccccgttaga aaagataggc
     3601 tgaaacttag aactaaaacg actaaaatgt catctaccac agaagcaccc aaaattgttg
     3661 atccttcttt tgtattcact acgtttagtt cttttaatac ttctactgaa atactgacag
     3721 aagttgccgc aagaataatc ccaaagaaaa tcgcttcatt tcccgaaact tggaatgctt
     3781 tgcctgtcaa ccagccaaaa acaaccggga agatgatccc aagcaaagca acgaacatac
     3841 caggtcggaa atattttttc aaaagagata aatcactttc cagaccagct aaaaacatta
     3901 ataagatcac accaatctcc gaaaagtcat ggactagaat atcaggtttg acccaattca
     3961 atccagcttg gcctagcaaa acaccaacaa gtaattgtcc aatcactgct gggataccta
     4021 atctccgcga gatatgtgag ccaatcgttg ttgaaatcaa tatcaggcat aaaataccaa
     4081 taaattccat tctcatcatt cctttgtata aaatatagcc cgaacttagc ataacataca
     4141 ttattgaaaa aataggaaaa tatactcaca aaaaaaccaa caatccaaaa gatttttttg
     4201 gattgttggt ttttatacgg tctattttaa taactaaaaa tgattaacgt atagtcattt
     4261 ttcttattct gatgaaattg ataatctcct ggatgcccat gaaaatcaaa ataaagccga
     4321 atatttggaa tagaagcatc aaacttttga atggattgaa caataagacg aatccgccaa
     4381 tcaacaacag aatactacta taaacactcc aacttttagc ttttgtattc atgctgaaga
     4441 acaactggaa caagctgttg atgataacag ctaatccaag aagaatcggc aggatagaaa
     4501 cgattgcagg tgcaaaagca tagacgatca gagctaagat caggaaaaaa agaccactga
     4561 acagaccgat actccaagaa cctgttttcc ttttaaaacg gtaagcctca aaaagattca
     4621 acaacccaag caaaacaaga taggcggtga tcagataacc gataaaatga aaaacagcag
     4681 aagggtttag cacaatcgca atcccagaaa taatataaac agcagctctt aataaggcgt
     4741 atttttgaat attttgttta atcgtctcaa acatataaga aaacctcctt tgacttattt
     4801 taacgtgaac tagtcatttt accaaattaa atgttacaaa atgagcgaag gagaagaata
     4861 tctctggaaa aatcaacttc tgaggatctt ttccattttt ttgccttttg ctagctcatc
     4921 gatcatttta tctaaataac gaatttcttt catcaagtta tcctcgatgt cttctactcg
     4981 tacaccacaa acaacacctg taatcaagga acgtgaagga ttggggacag gggcttccgc
     5041 aaaaaattgt tcgaaagtca cttgtttttg tagttgatat tctagttctg tttgggaata
     5101 acccgtcagc caacaaatga tttcatctac ttcctctttt atacgatctt ttctctccgc
     5161 ttttgctaca tacataggat aaacgcttgc aaaacttgtc gtaaaaatac gattttgcgc
     5221 cataatcagt cacctttctt tttttcaaat tatttactac ttatagtata taagaaaaag
     5281 agaagaaaaa gcgattattc aaaaaaacaa aaaggattgt gacaaaattc ttgtcaccat
     5341 ccttttttcc gctctctgac tttttacaat cttcatagct caagtgcgca tcagataatc
     5401 gaatgctcct aaagctgcag tagctcctga acccattgaa acaatgattt gtttgtatgg
     5461 actatctgta caatcaccag ctgcgaaaac gccggggata ttcgttgcgc catttttctc
     5521 ggtcactatt tcatttcttg gattcatttc gatcgttcca tctagccaat ccgtattagg
     5581 gagtaagccg actagaatga atactcctga aacagctagt gtatgcatct ccatcgtacg
     5641 gcgatcgata taggagagag attctacctg attcgtaccg ctgatttctt tcgtcgctgc
     5701 atgtttgata acagtgacat tcgcaagaga gactagttta tcctgtaaga tttgatctgc
     5761 ttttaattct ggtaagaatt ctagaacata tacatgtttt gctaacccag ccaaatcgat
     5821 tgctgcttca acaccagaat taccaccacc gataacagca atctcttttc ctttaaataa
     5881 agggccgtca cagtgtgggc aataagcaat tcctttattt ttaaactctt tttcgcctgg
     5941 tacattgata gaacgccagt gcgcacctgt cgcgatgatg gcggtttttg ctttaagtga
     6001 tataccgtta gctaagccaa tattaataaa atgttgctct ttatttaagg aaacggcctg
     6061 ttgattcatc ataatgtcga tagggtaact acgcacatga ctttctactt gctgcatcaa
     6121 ttttggacct tctgtgtaag aggtaccaat catattttca atccctagtg tttcaacgac
     6181 ttgtccgccg aaagaatcag cgacaagtcc tgtacggatt cctttacgtg ctgcatagat
     6241 agctgcacta ctagctgctg gtccaccgcc aattactaat acatcataag gttctttttc
     6301 aaacaactct tcttcaatca aagggccagt gattttttca acaatttgtt cgatcgtcat
     6361 gcgtccgcta ctaaattctt ctccatttaa aaacacagtt ggcactgcca tgattttttt
     6421 atcatcgatt tctttttgga acatcccacc ttcaatcatc gtatgagtaa tatttggatt
     6481 taatacagct aatatattca atgcctgtac aacgtcgggg caattatgac aagtcaagct
     6541 aacataactg tcaaaatgca atgtttgtgt tatttttttg attttaagac tcaaagattc
     6601 actgattttc ggtgcccgtc cactaatttg taacatagcc agtaaaaaag aggaaaattc
     6661 atgaccgagg gggataccag caaaaacgat tccactttct ttgccaactg gattgataga
     6721 aaaactaggt gtgcgcatcg aatctctttt ttcccaagag attttaggag acatttctgc
     6781 tatttccttc gtaaattcag ctagtttttg actattttca ctattatcca aactaacaga
     6841 aaaaacaata ggagattcaa tcagtgctaa atattgttcg agattttgtt tagtttcttt
     6901 gtctaacata tccaccacct aaatctttcc aacaagatca aaacttggtt tcaatgtttc
     6961 gccactttct ttccattttg ccggacaaac ttcacctggg tgggtacgta catattgagc
     7021 agcgcgaatc ttatcaatca gtgcactagc gtcacgaccg attccatctg cattgatttc
     7081 catagcttga acgattccgt ctggatcaat aataaatgtt cctcgctgag ctaatccttg
     7141 ctcttcatcc aaaacatcaa aaatacgaga aatctgatga gacggatcac cgatcatcac
     7201 gtattcgatt ttaccgatag catctgatgt atcatgccat gctttgtgag taaagtgggt
     7261 atcggtagag caagaaaaga cttcaacacc taaagatttc aatgtactgt attgttcttg
     7321 taagtcttct aattcagtag gacaaacaaa agtaaaatca gctggataga agcaaagaat
     7381 actccagttc cccataagat ctttttcact gacttctaaa aattctcctt gatgataagc
     7441 atgtgcatta aattcttcga tttttttccc aatcaatgac atagtaaaac ctccaataat
     7501 aattatttat ttataattat tgtaatttag ttggagttgc aaagtcaatg gatattttgt
     7561 tatttaataa agattactca aaaacgaagg ttaattataa aaatagaaga ggtcgcaaga
     7621 aaatgctact gctggaaata ctcgggataa cgttgagtaa cagtttcctg ttctttcagc
     7681 atcatgtgta agtgcaatcc cgtttgaaat cgagcttctt ctggtttttt atcaactaaa
     7741 gccttgagta aatcataatg ttgttccatg atcgtttccc atggaagccc ctgaatggtc
     7801 actcgtaacc agcggaaacg ttctaaatgg gtattatggt catctaacca gttccaaact
     7861 tcttttcttc ctgcaatttc gaaacaagct tcatgaaata aattatctgc acggaaaaaa
     7921 gcgcgttcgt catttatttt gactgctttt tcttgttctt ctaaaatcgt ttctagcact
     7981 attcgtgttt gtgtgtccaa tttggaacaa cattcaatca tgatttgctc ttccaactgt
     8041 tctcggacaa agcgagcatt ttctgcagaa acaagattaa ttttggaaac atacgtacca
     8101 ctttgaggta tcgtatacac gagttcttgc tgacgtagtt gaatcaatgc ttcgcgaatg
     8161 ggagtccggc cgatagcaag atgttcctcc agtgcctttt cagatatttt ccttccaggt
     8221 tctaagtcag catagataat ttgtttacga atcgcttgat aagcttgtga ttgcaagctg
     8281 cttttcatct ttattcctcc aaaatcattt ctatgaacgt agtataacag aagaggtctg
     8341 aaaaacaatc tggtatgtca cattaactag taaatttgta taaatagtaa aattttggtt
     8401 gacatagcga tttttattgt ataaaattca cttgaataca aaaactaata tgttagttta
     8461 ggaggggaaa aatgattgat ttaaacaact ggaggaactt tatccctgaa ttatctcaaa
     8521 aagaagtaga aatacctcat tttgaccgtg catcattaag agaaaatgga caaaaaaatc
     8581 ctgtctggat ccatttcggt ggcggaaatc tttatcgagg atttcatgca gaaatcgctc
     8641 aacgtttagc ggatctaggc gaattgtcca ctggtgtcat tgtttgtgaa acattcgatg
     8701 aagaaataat tgaaagggct taccaagatt ttgatgattt gattcttgaa gtggtcatgc
     8761 caagcagtgg agagttcgtc aaacgtatat tagctgcgac agctgacagt ctgtattgtc
     8821 atccagattg ttctgaacac tttgataaga tgattcgaca ctttgaaaat cctagtttac
     8881 aattagttac ttttgctatt actgaaaaag gttatcgatt gaaagataca aataataaat
     8941 atataggacc gattgcagtc gatataaaaa atggtccaaa gaagccatca catacaatga
     9001 gtattcttgc tgcgctactt tataaacgtt ttttatctgg agcttggccg attgcacttg
     9061 tctctacaga taatttttca caaaacggta gtcaattacg tacttctttg ctaacgatcg
     9121 caaaagaatg gcaaaaggaa ggttatgtac aaaatacatt tatccaatac ttatcgaatg
     9181 aagaaattgt tgcgtttcca tggacgatga ttgatcgtat caccccgaat ccttccttgt
     9241 cagttagcaa aatgttggca aaagagggct tttcaaatat gtcgattttc caaaccgcaa
     9301 aaggaaataa tattgctcca tttgtcaata cagaggacat ccattattta atcatggaag
     9361 atcattttcc caatggacgt ccgaaattcg aaaaagctgg ggtattcttg actgatcgtg
     9421 caacggtaga taaagcagat acgatgaagg tgacgacttg ccttaatcct ttgcatacgt
     9481 ctttagctat tatgggttgt ttactggggt ataggagcat agctgaggag atgaaagatc
     9541 cagatttatc gctgctagtt tctgaaatcg ggtacaatga aggcctccca gttgtggagt
     9601 ctccaggtat cattgatcca aagatctttc tggaagaagt aattactgtg cgcttttcta
     9661 atccaaatat tcctgatatg cctcagcgaa tcgccacgga tacctctcaa aaggtggcga
     9721 ttcgttatgg tgagacgatt aaaaaatata tggaaataga agttaaagac ccgcagacac
     9781 ttgtattcat accattgact ctcgctgctt ggcttcgata ccttattggc attgatgatc
     9841 aaggaaaacc attcatcccg agtccagacc cactattgac agactttcag cataagcttt
     9901 ccgatattca actgggtacg caagatacat cacatattca tgagaaaatg gctcctattt
     9961 tgtcaaataa agaaattttt ggtgtagatc tttaccaagc agagcttggc agaaaaatag
    10021 aagaaatgtt catcaagatg gtagcaggac cgaatgccgt acgaaaaaca ttacaggatt
    10081 acttacaaga acatggagga aatttatcat ggaaatgact tttagatggt acggatcaca
    10141 ggaagaaaag attaccttag atgaaatcag acagattcct gcggtcaaag ggattgtagg
    10201 cacattgatg gatcacagga agaaaagatt accttagatg aaatcagaca gattcctgcg
    10261 gtcaaaggga ttgtaggcac attgatggat ataccagtag gagaggtatg gcccagggag
    10321 aggatcaaag aactaaaaga agaaattgag tctcgtgggt taacactaaa agtaattgaa
    10381 agcgtcaatg tgcatgatgc cattaaaatt ggattacctt cccgtgagca gtatatcgaa
    10441 aactataagc agacaatccg aaacttggca gaatatggaa tagaagtcat ctgctataat
    10501 tttatgcctg tatttgactg ggtgaaatct gaccttgact accgcttaga agacggctca
    10561 tccacgttag cttttatatc tgatgatatt ccagatgatc caagagaaat cgtcgcgcgc
    10621 atcgaacatt ccagcaatga atttgaattg ccgggatggg aacctgagcg cttagctcat
    10681 atcaaatcct tgcttgaagc ctatgcatca gtagacgaag aaaaacttcg agaaaatttt
    10741 gcctattttc tccaatcaat catcccaaca tgtgaagagg taggggtcaa aatggctgta
    10801 catccagatg atccgccata ctcgattttt ggattacctc gaatcgtaaa gaatcgagaa
    10861 gatttagatt ggttgtgccg catagtagac agcccttata acggaatcac tctttgtaca
    10921 gggagtattg cagaagatcc agaaaatgat gtatatggga ttttagcaga attttgtcaa
    10981 agagatcgaa tcccatttgc acatgttcga aatattaaat ttttagctaa tcgagatttc
    11041 tatgaggcac cgcataaatc cgaatacggt tcattagaca tgtaccgcat actaaaaaca
    11101 atgtatgaca acggatttaa cgaatacatc cgtcctgatc atggaagaat gatctggaat
    11161 gagacaggaa gaccaggata cggcttatat gatcgagcat taggtgctgt ttatctaaat
    11221 ggattatggg aagcaatcga aaaaggaaac aaaagtaaag ataattagtg aaaaaaactg
    11281 taattttatt atgtaaataa gaaggatgtt caaattcttg atacataaat ccggaggttg
    11341 ttaataagca ttcggattta tgtatcttat ttggtctagg aagtaacttg attttctttt
    11401 ttcgattgat ccatgagtgc ttcttctttt aattcaaaga tatattctga gatcctcata
    11461 atcacttttt tccagtcttc tggtgtgatg attcctttta aaatatagat ttttttgata
    11521 tgagaaggca attgtttcaa agggcagtcg ctgataacga tgttgcagtt ctccataaaa
    11581 tcattttgta ttttgatcga ttcatttgaa aaaatttgtt ctatcttaac gatcagatct
    11641 ttactgatat aaaaatcacg cgtatagttc acaccaatca caattcgcgg ctgttttttg
    11701 tagcgatcat acaaatgtac taggtcttca accatccatt gcacattttc cttttctaaa
    11761 ataactggta acaagtgttg attttgttct ctgaaatcca tataaaattg ttcaatggcg
    11821 gtttggactt ctttcaacac gctatgttct tgataaagca tgaaagtagg aaggggcgta
    11881 tttggttgat tgaagttaag aaatccatta taaagtttat ttcgtgcgaa tgccaatgag
    11941 taaagaatct tttctttttc aggtataaac aaatcaaata cagtacaaaa ggactccacc
    12001 atttctcttg aataaagaac atgaggaacg tttgcttttt ttaaaagttt gtattgctcc
    12061 aagcttgttt cttccgattc gattttcgtg atagaaagtc gagctaaaat attcactaag
    12121 atgcgttgtt cgcgacaaat tggcagatta ggtcgcaaaa tatctacaga gggatcaata
    12181 aaaaagtcaa tatattcata ctctgtga
//
LOCUS       DACDKM010000083        12118 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20834_2016
            SAMN12640370-rid9891663.denovo.083, whole genome shotgun sequence.
ACCESSION   DACDKM010000083 DACDKM010000000
VERSION     DACDKM010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640370
            Sequence Read Archive: SRR10040891
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12118)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12118)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742835.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742835.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:04:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,012
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 29 of 75
            Pseudo Genes (incomplete)         :: 33 of 75
            Pseudo Genes (internal stop)      :: 19 of 75
            Pseudo Genes (multiple problems)  :: 5 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12118
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640370-rid9891663.denovo.083"
                     /isolate="E20834_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(81..638)
                     /gene="frr"
                     /locus_tag="IV334_002070"
     CDS             complement(81..638)
                     /gene="frr"
                     /locus_tag="IV334_002070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HAP8944128.1"
                     /translation="MADAIMTEAKEKMKKAAQNLQRELGQIRAGRANASLLDRITVNY
                     YGAPTPLNQMASIQIPEARVLMITPFDKSILQDVEKAIMASDIGISPTNDGNVIRLVI
                     PQLTEERRKELAKDVKKEAENAKIAIRNIRRDAIDEYKKQQKNGDITEDDLRGLEKDV
                     QTLTDNSIKEVDKIAADKEKELLEV"
     gene            complement(641..1363)
                     /locus_tag="IV334_002071"
     CDS             complement(641..1363)
                     /locus_tag="IV334_002071"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010761216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HAP8944129.1"
                     /translation="MVTPKYQRVVLKLSGEALAGDAGFGIKPPVIKEIIQEIKEVHEL
                     GVEMAIVVGGGNIWRGQIGAQMGMERAQADYMGMLATVMNALALQDTLENVGVPTRVQ
                     TSIEMRQIAEPYIRRKAERHLEKGRIVIFAGGTGNPYFSTDTTAALRAAEIGADVILM
                     AKNNVDGVYSADPKVDANAVKFEELTHLEVIAKGLQVMDSTASSLSMDNDIPLLVFNL
                     NEHGNIRRAILGENIGTTVRGK"
     gene            complement(1499..2380)
                     /locus_tag="IV334_002072"
     CDS             complement(1499..2380)
                     /locus_tag="IV334_002072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Ts"
                     /protein_id="HAP8944130.1"
                     /translation="MADVTAKMVKELREMTGVGMMDAKKALVEVEGDMEKAVDLLREK
                     GMAKAAKKNDRIAAEGLASVAVKGNTAAIVEVNSETDFVSKNEMFQDLVKEIAELVAE
                     NKPADMEAAMKIKTDKGTIESDLIEATQVIGEKISFRRFEVVEKDDNAAFGGYLHMGG
                     RIAVLTVLDGTTDETVARDVAMHVAAINPRYVNESQIPEAELEHEKTVLTEQALNEGK
                     PANIVEKMVEGRLKKFKAEIALVDQPFVKDPDMTVEKYVASKGATVKTFVRFEVGEGI
                     EKREDNFVEEVMSQVKK"
     gene            complement(2479..3261)
                     /gene="rpsB"
                     /locus_tag="IV334_002073"
     CDS             complement(2479..3261)
                     /gene="rpsB"
                     /locus_tag="IV334_002073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HAP8944131.1"
                     /translation="MAVISMKQLLEAGVHFGHQTRRWNPKMKKYIFTERNGIYIIDLQ
                     KTVKLVDAAYDYMKSVAEEGGVALFVGTKKQAQEAIKEEATRAGQYFVNHRWLGGTLT
                     NWDTIQKRISRLKQINAMEEDGTFEVLPKKEVAGLNKERERLEKFLGGIADMPRIPDV
                     MYIVDPRKERIAVQEAHKLNIPIVAMVDTNCDPDEIDVVIPSNDDAIRAVKLITSKMA
                     DAFIEGNQGEDQVVEEDFTEENTATSIEEIVDVVEGDNSSAE"
     gene            3620..4099
                     /locus_tag="IV334_002074"
     CDS             3620..4099
                     /locus_tag="IV334_002074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArgR family transcriptional regulator"
                     /protein_id="HAP8944132.1"
                     /translation="MRKAQRHLLIKQVIEEFPIRTQEELLNKLGHYGVTATQATISRD
                     IRDLKIVKAPDENGVSRFVLFREKGTPETRTEEEDRLVQMIEDIVLKVERVHFLTIVN
                     TLPDNAHLFASVLDEIKPPSIVSTIAGFDTIMVISENEEDAKRVEEFLHHPTEVSLF"
     gene            4316..5407
                     /gene="phnW"
                     /locus_tag="IV334_002075"
     CDS             4316..5407
                     /gene="phnW"
                     /locus_tag="IV334_002075"
                     /EC_number="2.6.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-aminoethylphosphonate--pyruvate transaminase"
                     /protein_id="HAP8944133.1"
                     /translation="MYKLLTSGPLTTSETVKNAMLIDHCTWDQEYKEITQWIRKKLLD
                     LAQVSEGIYTTVLMQGSSSFGVESVLSSVIRPEDTVLICSNGAYRQRMINMCECLKLN
                     YTVYEVSEHQILQAHVITQLIEENPSISALAMVHSETTSGILNNLEEIAAVTQERDLL
                     FIVDAMSSFGGIPICVAELGIDFLISSSNKCIQGVPGFSFVLANKAELEKRKNVSRSL
                     SLDLYDQYRVMENENGKWRFTSPTHTVLAFQQALKELEAEGGIETRYSRYLANNHVLR
                     QKMTEMGFQPFIQEYQGPFITSFLYPKDWRFDFNHFYQYLKQNGFVIYPGKISDHECF
                     RIGNIGEIYPEDILELTAIIKDYKENVND"
     gene            5400..5528
                     /locus_tag="IV334_002076"
     CDS             5400..5528
                     /locus_tag="IV334_002076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonoacetaldehyde hydrolase"
                     /protein_id="HAP8944134.1"
                     /translation="MIKTVIFDWAGTTVDFGCMAPVHAFRNAFLEKGIQLTDKEIR"
     gene            5532..6077
                     /locus_tag="IV334_002077"
     CDS             5532..6077
                     /locus_tag="IV334_002077"
                     /EC_number="3.11.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonoacetaldehyde hydrolase"
                     /protein_id="HAP8944135.1"
                     /translation="MGKLKWDHIQQLLSLPSVKKQWIAIYGQLPQEKEVEELYQQFEH
                     YLFKDLVKHSTLKPDTLETIERLRNAGINIGSTNGFNAPMMTIVAETAKKERYSPDFM
                     ATFEDVEGYGRPFPYMIHKNMQYFKNKSVDEVIKVGDTVSDIQEGKNAGVLTVGVIEG
                     SSLMGISYDEYQKLEMGKKIV"
     gene            complement(6531..8222)
                     /locus_tag="IV334_002078"
     CDS             complement(6531..8222)
                     /locus_tag="IV334_002078"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HAP8944136.1"
                     /translation="MNNKDLVAKAVYDAVKDDLTLEQVEQLLENPKSAEHGDVAFPAF
                     SLAKVYRKAPQQIAADLAEKIDSANFEKIEVVGPYLNFFMNKELISKKVLQTVVKEKE
                     HYGDSNIGNQGTVPIDMSSPNIAKPISMGHLRSTVIGNSIGFIMEKIGYQPIRINHLG
                     DWGTQFGKLIVAYKKWGTEEAVKAEPINELLRLYVQFHEVAETEPELNEEARAWFKRL
                     EEGDKEAIQLWQWFRDESMKEFNKIYDLLEVRFDSLNGEAFYNDKMDEIVKLLEEKHL
                     LNEDKGAEIVDLSAYDLNPALIKKSDGATLYITRDLAAALYRKRTYDFKQSLYVVGNE
                     QSYHFKQLKAVLKEMGFDWSDDMHHIPFGLITQGGKKLSTRKGKIVLLEEVLNEAIQS
                     AKEQISEKNPDLENKDAVAKQVGVGAVIFHDLKNDRLNTFDFNLEEVVRFEGETGPYV
                     QYTHARAVSLLEKAGFVPSETADYALNDDTSWEVVKLVQKYPETVLSAGEKYEPSVIA
                     KHAIKLAQAFNKYYAHTKILADDEQKEARLALVYAVTVLLKEDLRLLGLHAPDKM"
     gene            complement(8642..9589)
                     /gene="arcC"
                     /locus_tag="IV334_002079"
     CDS             complement(8642..9589)
                     /gene="arcC"
                     /locus_tag="IV334_002079"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HAP8944137.1"
                     /translation="MAKRKVVVALGGNAILSTDASAKAQQEALMETAKYLVKFIEQGD
                     ELIISHGNGPQVGNLLIQQQAADSEKTPAMPLDTCVAMTEGSIGYWLQNAMGEVLKEK
                     GIDKDVVSLVTQVIVDENDPSFKNPSKPVGPFYTEEEAKEQMNADSTVTFKEDAGRGW
                     RKVVASPKPISIKEARVIETLVDQGVITVSVGGGGIPVVETATGLEGREAVIDKDFAS
                     EKLAEIIDADLLIVLTGVDNVYVNYQKPDQKKLETVTVSEMKQYIDEKQFAPGSMLPK
                     VEAAIAFVEAKPNAKAIITSLENIENLLASEEGTIIVAD"
     gene            complement(9704..10723)
                     /gene="argF"
                     /locus_tag="IV334_002080"
     CDS             complement(9704..10723)
                     /gene="argF"
                     /locus_tag="IV334_002080"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003073325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HAP8944138.1"
                     /translation="MKESVFQGRSLLAEKDFTKEELQYLIDFSEHLKDLKKRGIPHHY
                     LEGKNIALLFEKTSTRTRSAFTTAAIDLGAHPEYLGANDIQLGKKESTEDTAKVLGRM
                     FDGIEFRGFSQKMVEELAEFSGVPVWNGLTDEWHPTQMIADFLTIQENFGTVEGITVA
                     YCGDGRNNMANSLLVTGAILGANMRIVAPKELQPEEEIVKMAEGFAEKSGAQLMITDD
                     VDKGVDGADVLYSDVWVSMGEEDKFEERIKLLKPYQINMEMVEKTHNTDRLIFLHCLP
                     AFHDTNTVYGEQMKERFGITEMEVTDEVFRSKYARQFDQAENRMHSIKAIMAATLGNL
                     FIPRV"
     gene            complement(10814..12043)
                     /gene="arcA"
                     /locus_tag="IV334_002081"
     CDS             complement(10814..12043)
                     /gene="arcA"
                     /locus_tag="IV334_002081"
                     /EC_number="3.5.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine deiminase"
                     /protein_id="HAP8944139.1"
                     /translation="MDKPIHVFSEIGKLKTVLLKRPGQEVENLTPDIMDRLLFDDIPY
                     LPIAQEEHDNFAKTLQNEGVETLYLEKLAAEAIDAGNVKEQFLNKMLDESHIASNAVR
                     EGLHEFLLSMETQEMVDKIMAGVRTKDIEVRSSSLYDLSADDDYPFYMDPMPNLYFTR
                     DPSASMGNGMTVNKMTFEARRRESMFTEYILKHHPRFANKGIEVWLDRENPDHIEGGD
                     ELILSDKVVAVGISQRTNAKALEKLARHLFAKNSGFEKVLAIKIPNNRAMMHLDTVFT
                     MVDHDKFTIHPAIQSKDGKMDVFTIEPDGDDIKITHSDNLHETMKAALGLDDLVLIPT
                     GNGDEIVAPREQWNDGSNTLAIAPGVVVTYNRNYVSNELLRSYGIKVLEINSSELSRG
                     RGGPRCMSQPLVREDLK"
BASE COUNT         3311 a         2517 c         2197 g         4093 t
ORIGIN      
        1 ttttttaatt tttaaataaa agaaatataa ataaaaacag aggaagcgga atgatccgcc
       61 cctctgtttt tatgatttta ttaaacttcc aataattctt tttctttatc agcagcgatc
      121 ttatctactt ctttgatgct gttatctgtc aatgtttgta cgtctttttc taatccgcgc
      181 aaatcatctt cggtaatatc accatttttt tgttgttttt tatattcatc gatcgcatca
      241 cgacggatgt tacgaatcgc gatttttgca ttttctgctt cttttttaac atctttagct
      301 aattcttttc tgcgttcttc tgtcagttgc gggataacta agcgaatgac atttccatca
      361 ttggttggac tgattccgat atcacttgcc ataatagctt tttcaacatc ttgaagaatg
      421 ctcttgtcaa aaggtgtgat cattaaaaca cgtgcttctg gaatttggat tgatgccatt
      481 tggttcaatg gtgttggcgc accgtagtag ttcacagtaa tacggtccaa aaggctagcg
      541 ttcgcacggc ctgcgcggat ttgcccaagt tcacgttgaa gattttgtgc tgcttttttc
      601 attttttctt ttgcttctgt cataattgca tctgccattt ttatttcccc cttacagttg
      661 ttccgatatt ttcgcctaag attgcacggc gaatatttcc gtgttcattt aaattgaaga
      721 caagcagtgg aatatcatta tccatgctta gtgaacttgc agttgaatcc atgacttgta
      781 atcctttggc aataacttcc agatgagtca gttcttcaaa ttttactgca tttgcatcta
      841 ctttcggatc agcagaataa acaccgtcta cattgttttt agccatcagg ataacatctg
      901 cgccgatttc tgccgcgcgt aatgctgctg ttgtgtctgt cgagaagtac gggttgccgg
      961 taccgcctgc aaagataacg atccgtcctt tttctaaatg acgttctgct ttacggcgaa
     1021 tatatggctc tgcgatttga cgcatttcaa tcgaagtttg cacgcgtgtc ggtacaccca
     1081 cgttttccaa cgtatcctgt aaagctaagg cattcataac tgtagctaac atacccatgt
     1141 aatcagcttg agcacgttcc atgcccattt gtgctccgat ttgtccgcgc cagatatttc
     1201 cgccgcccac aacgattgcc atttcaacgc ccagctcgtg aacttctttg atttcttgga
     1261 tgatttcttt gattactggt ggtttgattc caaatcctgc gtccccagct aaagcttctc
     1321 cactcaattt taagactaca cgctgatatt tcggtgtgac catgttcatt cctccaatat
     1381 tttctattgc cattttacca tacttagatg atttcgacac tctaagactg gtttttcttg
     1441 ttttgcttaa aaaaagggag cgcacaaacc gtgcgttccc gtctgttatt caacagaatt
     1501 attttttcac ttggctcata acttcttcta caaagttatc ttcgcgtttt tcgatacctt
     1561 cgccaacttc gaaacgtacg aatgttttaa cagttgcgcc ttttgatgca acgtattttt
     1621 caacagtcat atcaggatct ttaacgaatg gttggtccac caaagcgatt tctgctttga
     1681 atttcttcaa gcgtccttct accatttttt caacgatatt cgctggtttg ccttcgttca
     1741 atgcttgttc tgtaagaaca gttttttcat gttctaattc agcttctgga atttgtgatt
     1801 cgtttacata acgaggattg atagctgcta cgtgcatagc tacgtctctt gcaactgttt
     1861 cgtcagttgt tccatctaga acagtcaata cggcaatacg tccgcccata tgcaagtatc
     1921 caccaaatgc agcattgtcg tctttttcta caacttcaaa gcgacggaag ctgatttttt
     1981 ctccgataac ttgagttgct tcgatcaagt cagattcaat tgtaccttta tctgttttga
     2041 ttttcattgc agcttccatg tctgctggtt tgttttcagc aacaagttct gcaatttctt
     2101 ttactaaatc ttggaacatt tcgtttttag aaacaaagtc tgtttctgag tttacttcaa
     2161 cgattgctgc agtgttacct ttcacagcaa ctgaagccaa tccttcagct gcaatgcggt
     2221 catttttctt cgcagccttt gccattcctt tttcacgtaa aagatcaact gctttttcca
     2281 tgtcgccttc tacttcaact aatgcttttt tcgcatccat cattcctacg ccagtcattt
     2341 cgcgtagttc ttttaccatt ttagctgtaa cgtctgccat gtttaactcc tcctgtgtgt
     2401 ttttcttttt aaaaaaagct gcttcaaagg agagtgaggc ttgtcgccca gcctttgaaa
     2461 cagctccatc attcaaaatt attctgctga agaattgtcg ccttcaacaa catctacgat
     2521 ttcttcgatt gatgttgctg tgttttcttc tgtgaaatct tcttcaacta cttgatcttc
     2581 accttggttt ccttcgatga aagcatcagc catttttgaa gtaatcaatt taacggcacg
     2641 aattgcatcg tcgtttgaag ggattactac gtcgatttca tctggatcgc agtttgtatc
     2701 aaccatagct acgattggga tatttaattt gtgtgcttct tgaacagcaa tacgttcttt
     2761 acgtgggtca acgatgtaca ttacatctgg gattcttggc atatcagcga taccgcctaa
     2821 gaatttttca agacgttcac gttctttgtt caaaccagca acttcttttt taggtagaac
     2881 ttcaaaagtt ccatcttctt ccattgcatt gatttgtttc aaacggctga tacgtttttg
     2941 gatagtatcc cagtttgtca atgttccgcc taaccaacga tggttaacga agtattgtcc
     3001 tgcacgagtt gcttcttctt tgattgcttc ttgtgcttgt ttttttgtac ctacgaataa
     3061 tgctacgccg ccttcttcag caacgctctt catgtaatca taagctgcat ctactaattt
     3121 aactgttttt tgcaagtcaa tgatgtagat tccgtttctt tctgtgaaga tatatttctt
     3181 catttttgga ttccagcgac gagtttggtg accaaagtgt acgccggctt ctagtaattg
     3241 tttcatagaa attactgcca tgtttgtttc ctccgatttg gttttatttt tcctcttctt
     3301 atcttctacc ttgccggcaa actttttcaa gcacctaccg acaatccaat aagaatgtgg
     3361 atttgctgat gcataaagca ccctcagcta ttatacagac aaaagatccg tatcgcaaga
     3421 gcaaatatac ttttctgctc aacattcaat agtttacctg aaagataaaa agaattcaag
     3481 aaaaacagta gatttctctt tgtttcttta ttatcctttt tccataaaat aattttaggt
     3541 ataaaagacg aatattattg ggcaatcctt cttttttttg ttatgataca tgtgaaatct
     3601 ttcgtaaagg agaaagaaaa tgcgcaaagc tcaaagacat ttgctgatta aacaagtcat
     3661 tgaagaattc ccgatccgta cgcaagaaga actattaaac aaacttgggc attatggcgt
     3721 taccgccacg caggcaacga tttctcgaga tatccgtgat ctaaaaatcg tgaaggcacc
     3781 tgatgaaaat ggcgtttccc gcttcgtatt gtttcgcgaa aaaggaactc ctgagactag
     3841 gactgaggaa gaggatcggc tggttcaaat gattgaagac attgtattga aagtcgaacg
     3901 agtccacttt ttgacgatcg tcaatacctt gccagacaat gctcatttat ttgcatctgt
     3961 tttagatgag atcaaacctc cctctattgt cagtacaatt gctggattcg atacgatcat
     4021 ggtcatctcg gaaaatgagg aggacgcaaa acgagtagaa gaatttcttc accatccaac
     4081 tgaagtatca cttttttaat cagagcacgt attcctaata tggaatgcgt gtttttttat
     4141 tttcaatatt ttccttattg aataaacctt tacactcttt acaaaaatac atcatttttt
     4201 attcaaattt ttcaacacct tgattgaatc acaataatca tcatttatat tggatgttaa
     4261 caagagcagc ttcttgctct ttgtgtattt caaaaaagaa atgaaaggaa atcatatgta
     4321 taaattatta acttctggac cattgacgac ttctgaaaca gtgaaaaatg ctatgctgat
     4381 cgatcattgt acttgggatc aagaatacaa agaaattaca caatggataa gaaaaaaatt
     4441 attggatctt gctcaagtat cggaaggaat atacacaacg gtcttaatgc aaggtagcag
     4501 ctccttcggg gtagaatcgg tattaagttc tgtgatcagg ccagaagata ctgtactgat
     4561 ttgttcaaac ggcgcatata gacaacgaat gatcaacatg tgcgaatgtc tgaaactgaa
     4621 ctataccgtt tatgaggtat cggaacatca aatcctccaa gctcatgtga ttacacagct
     4681 gattgaagag aatccgtcta tctccgcact agcaatggtc catagcgaga ctacttccgg
     4741 tattttgaat aatttggaag agattgctgc tgtcacacaa gaaagagatc ttttgtttat
     4801 agtggacgcg atgtcaagct ttggtgggat accgatttgt gtagccgaac tagggattga
     4861 ttttttgatt tccagctcaa acaaatgtat tcaaggtgtt ccaggctttt cttttgtact
     4921 tgctaataaa gcggaactgg aaaaacgaaa aaatgttagc cgaagtttgt cacttgatct
     4981 atatgaccag taccgcgtga tggaaaacga gaatggcaaa tggcgtttca cttcccctac
     5041 ccatacggtt ttggcttttc aacaagcgct gaaggaacta gaggcagaag gaggaatcga
     5101 aactcgatat tcacgttatc tcgccaataa tcatgtgcta cgacaaaaaa tgacagagat
     5161 ggggttccag cctttcattc aagaatatca ggggccattc atcacctctt ttctttaccc
     5221 taaggactgg cgatttgatt ttaatcattt ttatcagtat ttaaagcaaa atggatttgt
     5281 gatctatccc gggaaaatca gcgatcatga atgttttcgt atcggaaata ttggcgagat
     5341 ctatccggaa gatattttgg aactgacagc aattatcaaa gactataagg agaacgttaa
     5401 tgattaaaac tgtaattttt gattgggcag gaacaactgt ggactttggc tgtatggctc
     5461 ctgtccacgc ttttagaaat gcttttctag aaaaaggaat ccaactgact gacaaagaaa
     5521 tccgctaacc gatgggaaaa ttaaaatggg atcatatcca acagcttctt tccctccctt
     5581 ctgtcaaaaa acaatggata gctatttatg gacaactacc tcaggaaaaa gaggtggagg
     5641 aattgtatca acaatttgaa cattatctgt ttaaagattt ggtcaagcat tctaccttga
     5701 aacccgacac attggaaacg atcgaacggt tgagaaacgc tgggatcaat attggttcca
     5761 cgaatggatt taatgctcct atgatgacaa tcgtagcaga aaccgcaaaa aaagaaaggt
     5821 attcaccaga cttcatggca acttttgaag acgtcgaagg gtatggtcga ccgtttcctt
     5881 atatgatcca taaaaacatg cagtacttca aaaacaagtc ggttgatgaa gtaatcaaag
     5941 tcggagatac ggtctcagat atccaggaag ggaaaaatgc cggagtgttg acagtaggtg
     6001 taattgaagg tagttcgcta atggggattt cttatgatga gtaccaaaaa ttagagatgg
     6061 ggaaaaagat cgtttaaaac agtctgttgc tcttagattc caacaagccg gcgctgatat
     6121 ttgtgtaaac aatttaaaag agttaacaga atacattttg tacgctgaca tgaatccaga
     6181 atgactttta tacaaaataa gaggttgtga aaaagctgtc ctgtatcaag taataaaaac
     6241 agccaagcaa aaatagctcc tcatattttt cgtttggtta ggcttattac cgcagatgca
     6301 agcttttgaa caccgtttat tcgaaaaaaa aaagggagct gtgacacgac ttttgtcaca
     6361 gtccccttat ttgcaaacaa acagtgttta agagacaacc tctccactaa acggtgttca
     6421 ttcagctgac tctcggtatt tgctcaaaaa acgataaaat ttgaaaagct attttctctt
     6481 ttgtttctct aatactcgga tcagaacgct gatttcacat tctctttttc ttacatctta
     6541 tctggtgcat gaagtcctag caaacgaagg tcttctttca ataatacagt cactgcataa
     6601 actagagcta agcgtgcttc tttttgttca tcatcagcta aaatcttcgt atgagcatag
     6661 tatttgttga atgcttgtgc taatttgatt gcatgtttcg caatcactga cggttcgtat
     6721 ttttccccag cagacaagac cgtttcaggg tatttttgaa ctaatttaac cacttcccag
     6781 ctggtatcgt cattcaatgc atagtctgct gtttctgaag ggacaaatcc tgctttttcc
     6841 aacaggctga ctgcacgagc atgtgtatat tgcacataag gtccagtttc tccttcaaat
     6901 cgaacaactt cttccaggtt aaagtcaaat gtattcagtc gatcgttttt caaatcgtgg
     6961 aaaatgactg ccccaacccc aacttgttta gctacagcat ctttgttttc caaatcaggg
     7021 tttttctcgc tgatttgttc tttagcagat tggattgctt catttaatac ttcttctaat
     7081 aagacgatct ttcctttacg agttgataat tttttaccac cttgcgtgat caaaccaaat
     7141 ggaatatggt gcatgtcgtc agaccagtca aagcccattt ccttcaagac agctttcaac
     7201 tgtttaaaat gatagctttg ttcattccct actacatata gagattgttt gaagtcgtaa
     7261 gtacgtttac ggtacaatgc agcagctaag tcacgtgtaa tgtatagtgt cgcaccgtct
     7321 gacttcttaa tcaacgctgg attcaggtca taagcagata ggtcaacgat ctctgcacct
     7381 ttatcctcgt tcagcaaatg tttttcttct aaaagtttga cgatttcatc catcttgtca
     7441 ttgtagaaag cttcaccgtt caacgaatcg aaacgtactt ccagcagatc atagatttta
     7501 ttgaattctt tcatcgattc atcacggaac cattgccata attgaatagc ctctttatcg
     7561 ccttcttcca gacgtttgaa ccaagcacga gcttcttcat ttaattctgg ttctgtttca
     7621 gctacttcat ggaattggac gtataaacga agcaattcat tgattggttc tgctttaaca
     7681 gcttcttctg taccccattt tttgtaagcg acgatcagtt ttccaaactg tgttccccag
     7741 tcacctaaat ggttgatgcg gattggctga taaccgattt tttccataat gaaaccgatc
     7801 gaattaccga taactgtcga acgcaaatgt cccattgaga ttggtttggc aatattagga
     7861 gaagacatgt cgattggtac tgttccttga ttcccgatgt tgctatcccc ataatgttct
     7921 ttttctttca ctactgtttg caaaactttt ttgctgataa gttctttatt catgaaaaag
     7981 ttcaagtaag gtccgacaac ttcgattttt tcaaagttgg cagaatcgat tttttctgct
     8041 aaatctgcag cgatttgctg tggcgctttt cggtaaactt ttgctaatga aaaggctgga
     8101 aatgcaacat ctccgtgttc agctgatttt ggattttcta gtaattgctc gacttgttcc
     8161 agtgttaaat catctttgac agcgtcatag acagcttttg cgacaagatc tttattattc
     8221 atctttattc ctcctaaagt gttcccttga ttgttaaaca aaaaaaatct ctagcgacgg
     8281 catacttagt atgccatcac tagagacgag atttcccgcg gtaccactcc aattgtccta
     8341 aaaaaggacc agctccatct gatatcggta gacgaccgtc tgtttaaaac agaattctcc
     8401 aagtgcgctt cgttgtatct tcttcattcg gcttccacca cttccgaatt cgctagccaa
     8461 gaatgataca agtactctct tgttcaacaa atcttagtct tattaaactt tcttgaatta
     8521 tatcagaaaa aaaaacgaaa ggctagtcgt ttcataaaaa aacaatccag atggcctaaa
     8581 ggagaagaaa taccacctgg atagctataa atcatttcgt aaatacgaaa gacttaacaa
     8641 attaatcagc tacgataatc gttccttctt cagaagctaa aagattctcg atattttcta
     8701 aagaagtgat gattgcctta gcgtttggtt ttgcttcaac aaatgcaata gctgcttcta
     8761 ctttcggaag catgcttcct ggagcgaatt gcttttcatc aatgtattgt ttcatttctg
     8821 aaacagtaac agtttctaat ttcttttgat ctggtttttg gtaattgaca tacacattgt
     8881 ctactcctgt caatacgatc agcaaatccg catcgatgat ttcggctaat ttttctgaag
     8941 caaaatcttt atcgattacg gcttctcgtc cttctaatcc agtagctgtt tctacaacag
     9001 gaatcccacc gccaccaaca gagactgtga taactccttg atcgaccagc gtttcgatga
     9061 cacgtgcttc tttaatcgag atgggtttag gggaagcgac tacttttctc catccacgtc
     9121 cagcatcttc tttgaatgtc acagtggagt cagcattcat ttgttctttg gcttcttctt
     9181 ccgtgtagaa tggaccgact ggtttacttg gatttttgaa tgaaggatca ttttcatcca
     9241 caataacttg agtgactaaa gaaaccacat ctttatcgat ccctttttcc ttcagtactt
     9301 cgcccatggc gttttgtaac cagtaaccga tagatccttc tgtcattgca acacatgtat
     9361 ctaaaggcat tgccggtgtt ttctcagaat cagcagcttg ctgctgaatc aataggttac
     9421 cgacttgcgg accatttccg tgggagatga tcaattcatc tccttgttcg ataaatttca
     9481 ctaaatactt tgctgtttcc attaacgctt cttgttgggc ttttgcactt gcatccgtag
     9541 acaagatggc attgccccct aaagcgacaa ctacttttcg tttggccatt tcattcgtcc
     9601 tttcttcagt tctaccgaat tttctagata acaaaacgag ccagcgtttt gtgattttcc
     9661 ctcaatgctg actcgttttt tcgtgagatg aatgtggtta tgcttaaacg cgagggataa
     9721 acaagttacc taatgttgct gccatgatcg ctttgattga gtgcatacga ttttctgctt
     9781 ggtcaaattg acgagcatat ttgctgcgga agacttcatc tgttacttcc atttcagtga
     9841 tgccaaagcg ttctttcatt tgttcaccgt aaacagtgtt tgtatcatgg aaagctggca
     9901 agcagtgcaa gaagatcaaa cgatctgtgt tgtgtgtttt ttctaccatt tccatattga
     9961 tttggtaagg tttcaacaat ttgatacgtt cttcgaattt atcttcttca cccattgata
    10021 cccatacatc agaataaagg acgtctgctc cgtctacacc tttatcaaca tcgtcggtaa
    10081 tcatcaattg tgcaccggat ttttcagcaa agccttctgc cattttgacg atttcttctt
    10141 caggttgtaa ttcttttggt gcaacgatac gcatattcgc tcctaaaatt gcacctgtga
    10201 ctaataaaga gttagccata ttgtttcgac catctccgca gtaagcaaca gtgatacctt
    10261 ctactgttcc aaagttttct tggatagtta aaaagtcagc gatcatttgt gttggatgcc
    10321 attcatcagt caagccattc caaactggaa caccagagaa ttctgctagt tcctctacca
    10381 ttttttgact gaatccacgg aattcgattc cgtcaaacat acgtcctagt actttagctg
    10441 tatcttctgt ggattctttt ttacctaatt ggatatcatt tgcacctaga tattcaggat
    10501 gtgcacctag atcgatcgct gcagtagtaa atgcagaacg agtacgtgta gatgtttttt
    10561 caaataaaag cgcaatattt tttccttcta gatagtgatg tgggatacca cgttttttca
    10621 agtctttcaa gtgttctgaa aaatcgatca aatattgtaa ttcttctttt gtaaagtctt
    10681 tttctgctaa taagcttctt ccttggaata ctgattcttt catattaaac attctccttt
    10741 agattgtttt tatttaagat taaagaggaa atcccgacgg cagctgacag taagactgcc
    10801 gtcaggaaac aaattacttc aagtcttcac ggacaagtgg ttggctcatg cagcgtggac
    10861 caccacgacc tcgtgacaat tcacttgaat tgatttctaa cactttgatg ccatagctac
    10921 gaagcaattc gttcgataca taatttcggt tataagtgac tacaacacct ggagcgattg
    10981 ccaatgtgtt tgatccatca ttccattgtt ctcgaggtgc aacgatctca tctccattac
    11041 cagttgggat cagtacaaga tcatctaatc caagagcagc tttcatcgtt tcgtgtaagt
    11101 tatctgaatg agtaatcttg atatcatcac catctggttc gattgtaaag acatccatct
    11161 taccgtcttt actttgaatt gctggatgga tagtgaattt atcgtgatct accattgtaa
    11221 atacggtatc caaatgcatc attgcacggt tgttcggaat tttgattgcc aacacttttt
    11281 caaatcctga attttttgcg aataggtggc gtgctaattt ttctaaagct ttcgcattag
    11341 ttcgttgaga aatcccaaca gctactactt tatcgcttaa gatcaattcg tctccgcctt
    11401 cgatatgatc tggattttca cgatccaacc atacttcgat tcctttattt gcaaatcttg
    11461 gatgatgttt caaaatatat tctgtaaaca tcgattcacg acgacgtgct tcaaaagtca
    11521 tcttattgac tgtcatccca tttcccattg atgcggaagg gtcgcgtgta aagtacaagt
    11581 ttggcattgg gtccatgtaa aatggatagt catcgtctgc tgacagatca tatagactgc
    11641 tagaacgtac ttcgatatct tttgtacgaa ccccagccat gattttatct accatttctt
    11701 gcgtttccat tgatagaagg aactcatgaa gaccctctct tacagcattc gatgcaatat
    11761 gagattcatc caacatttta ttcaagaatt gttctttgac attgcctgcg tcaatcgctt
    11821 ctgccgctaa tttttccaag taaagagtct cgacgccttc attttgcaat gtcttcgcaa
    11881 agttgtcatg ttcttcttga gcaatcggaa gatatggaat atcatcgaac aacagacgat
    11941 ccataatatc tggtgtcaaa ttttccactt cttgtccagg ccgtttcagt aaaactgtct
    12001 ttaatttccc tatttcagag aaaacgtgaa taggtttatc catgttgtgt tgcctccttc
    12061 tttttgatta catgtatagg atatcctcct tttgtaatca ctttcataat tttgtctc
//
LOCUS       DACDKM010000084        11995 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20834_2016
            SAMN12640370-rid9891663.denovo.084, whole genome shotgun sequence.
ACCESSION   DACDKM010000084 DACDKM010000000
VERSION     DACDKM010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640370
            Sequence Read Archive: SRR10040891
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11995)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11995)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742835.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742835.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:04:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,012
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 29 of 75
            Pseudo Genes (incomplete)         :: 33 of 75
            Pseudo Genes (internal stop)      :: 19 of 75
            Pseudo Genes (multiple problems)  :: 5 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11995
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640370-rid9891663.denovo.084"
                     /isolate="E20834_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(<1..490)
                     /locus_tag="IV334_002082"
     CDS             complement(<1..490)
                     /locus_tag="IV334_002082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YSIRK-targeted surface antigen transcriptional
                     regulator"
                     /protein_id="HAP8944140.1"
                     /translation="MFSDKEVLLKSLHVCLKLPIVVFNSEFSIIKEYRSDRTTYFFYD
                     YKKLLTDNIKKTKDFHFFTGDFNEMFLLYMFKTRYYLFGPFRANNIDKVFFKLKMNNL
                     NVAMADRERLYNSLQNLTLYSLGDIRDILILVHYFFTGKIEDLFHEPLIEYTENLSKT
                     IEQ"
     gene            1184..1570
                     /locus_tag="IV334_002083"
     CDS             1184..1570
                     /locus_tag="IV334_002083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HAP8944141.1"
                     /translation="MSDNDERVNAQLIRFFEKVILNTANTYYKKQEEIQKHEQLDQLP
                     PYLFTKDTIKFKQPINVLNVTLLVEDIKLAKILPLLKEPEQIFLIEKFIFDKTDKEIG
                     KVLGITRQGATNLKHRLYKKIQKMLV"
     gene            1664..1855
                     /locus_tag="IV334_002084"
     CDS             1664..1855
                     /locus_tag="IV334_002084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP8944142.1"
                     /translation="MNSLKEDFILAKAGNEEAVEAILKRFSSLIHKQSWRTGKYDQDC
                     YQECMLAIYLAISKFEIKE"
     gene            2369..2701
                     /locus_tag="IV334_002085"
     CDS             2369..2701
                     /locus_tag="IV334_002085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAP8944143.1"
                     /translation="MRKTYDRKFKLKISQDILEKKVTTKKIAEEYNISRPTISRWVSE
                     YRRYGKNAFAGQGKRLPDKADFYILEQENKRLKEEDECSTNTKIDTDLQGSLKNFTMK
                     ESKSINVL"
     gene            2997..3152
                     /locus_tag="IV334_002086"
     CDS             2997..3152
                     /locus_tag="IV334_002086"
                     /inference="COORDINATES: protein motif:HMM:NF012870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
                     /protein_id="HAP8944144.1"
                     /translation="MPTVIHSDRGSQHRSFIYQELLNDHHITHSVSQTGTPVDNAVIE
                     AFHRSIK"
     gene            3428..4768
                     /locus_tag="IV334_002087"
                     /pseudo
     CDS             3428..4768
                     /locus_tag="IV334_002087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303667.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
     gene            complement(5009..6625)
                     /locus_tag="IV334_002088"
     CDS             complement(5009..6625)
                     /locus_tag="IV334_002088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002406986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HAP8944145.1"
                     /translation="MKKIPITILTGYLGSGKTILLNHILYGDHGKEIAVIVNDIGEVN
                     VDGVLVDKKGFKRTGEKLVSMPNGCICCTLREDLIVELDQLVQFSDLNQIVIEASGIS
                     KPIPIIQAIILAKNPLDIDLTQRLEIGAIIIVVDVKRFWDNFETGEKIQERKAEGNVV
                     EEEQDIRGLLIDQFECCNILLLNNSDLVSTKTIYKINGLIAKLQSNAKIIRTIKGEVD
                     LSKILNTNLFNIDETSYSVGWFKELELGYQNHIPETEEYGISSFIYRNRLLLDAQKFA
                     HLTDKNFPGNITRAKGFLWFAQHSDFSTIFETSGSYKSIESLNYWVASLPKIECQMII
                     QKNPELKEAWHPLFGERINKLVFIGMKMDQQCIKEQLEGCLTDSLNADNFRKISPFPW
                     KKSNMFSQISLMKFLRKAGVKIGDVIIIGILIITSFIPLFVFSIEKENETTEDAIKYA
                     IVRIDGKEMDRFDLDKINHKLVNYHPAKGQYNIVEIKDGRIRVKEDNSPDQIAVRTGW
                     ISKPGQTSICLPHKLVISIEKKESTDYYIY"
     gene            6743..6961
                     /locus_tag="IV334_002089"
     CDS             6743..6961
                     /locus_tag="IV334_002089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP8944146.1"
                     /translation="MSQYRHLTILERERIFLLHEEGFSISIIGNDIQHSPFTILHELR
                     RNKKKTIHLPKRKKIMLIEEKNADVITF"
     gene            7156..7617
                     /locus_tag="IV334_002090"
     CDS             7156..7617
                     /locus_tag="IV334_002090"
                     /inference="COORDINATES: protein motif:HMM:NF033563.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HAP8944147.1"
                     /translation="MHHRGKTRHTKNHVESRGKICLTHSIHGRPKSADDRSDFGHWKA
                     DIVAGKTGGTCLVTLTDRKSCFLLAGKKVPRKKAAYVSEKMIELLSSIPNEAVKTITP
                     DRGKEFSNHFAVTEALNNMPFYFPDPHALGNNEQIKIRIDSFENTCQNRKR"
     gene            complement(7810..8049)
                     /locus_tag="IV334_002091"
     CDS             complement(7810..8049)
                     /locus_tag="IV334_002091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8944148.1"
                     /translation="MVRKVLLSMDSKNIDAYRRYRFAKQMRLNGSTLADIAATLHVSI
                     DTVHRVLKRDPDCCFIKVNTGRSRRLAKRHNSLAN"
     gene            complement(8073..9596)
                     /locus_tag="IV334_002092"
     CDS             complement(8073..9596)
                     /locus_tag="IV334_002092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     AdcA"
                     /protein_id="HAP8944149.1"
                     /translation="MKKISVGLIGIAALGLLGACSSTNDAKVSNDKDGKLEIVTTFYP
                     MYDFTKNIVGDEANVDLMVPAGSEPHDYEPSAKDMAKAHDADVFVYHNENMESWVPKA
                     KESWKKAGPNVVEGTKDMILLPGSEEEDHDHGEEDHHHELDPHTWVSPKMAIKEVSNI
                     KDQLVKLYPKKAKVFETNAEKYLTKLKRLDADYTTSLKEAKQKSFVTQHAAFGYLALD
                     YGLKQVPIAGLSPEEEPSSGRLAELKEYVKKNKINYIYFEKNANDKIAKTLANEAGIK
                     LEVLNPLESLTKEQMDNGEDYVSVMEDNLKALEKTTMVAGEEVVPEKEAKDEKTVANG
                     YFKDADVKDRELSDYTGEWQSVYPLLKDGILDEVFDYKAKINKDMTAAEYKDYYTTGY
                     KTDIDTINIKDNTIDFVVNGEHHQYTYKYVGYKILNYEKGNRGVRFNFETDDAGAGRF
                     KYIQFSDHGIAPSKAEHFHIFFGGESQEKLYNELHNWPTFYPASLSKHEIAQEMMAH"
     gene            complement(9614..9736)
                     /locus_tag="IV334_002093"
     CDS             complement(9614..9736)
                     /locus_tag="IV334_002093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative metal homeostasis protein"
                     /protein_id="HAP8944150.1"
                     /translation="MEKTDISSAYRRLKSPNIKTRKRALKIIKEHKRNKMKKLA"
     gene            complement(9766..10749)
                     /locus_tag="IV334_002094"
     CDS             complement(9766..10749)
                     /locus_tag="IV334_002094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HAP8944151.1"
                     /translation="MGIPVTIVSGFLGAGKTTLINQALQVSPYPREEIIIIENEFGEV
                     GIDHKLLVHSEERVLQLNNGCMCCSLRSDLLAILISLLETIEEHQQPISQIIIETTGI
                     ADPQPIIQTLLTAPQLRGRVYIDSLLTVIDSDSFELIEREPQALKQLVMADRIFVSRK
                     QNLNLSALKKLQREIKSINPLSDIRMFTHSETIQKKDFYNLKKFNHPLVFESDSSENH
                     YNGKYYHQHHQVHEFKSILLTSDQDIKKDFLLQWIDWLILTNQGSLYRFKGLIALQEQ
                     DFIVAMQEVNEQVNFQYTTRQKDQTTIILIGKMLDEERIKQSFDKLLAESK"
     gene            complement(10773..10922)
                     /gene="rpmG"
                     /locus_tag="IV334_002095"
     CDS             complement(10773..10922)
                     /gene="rpmG"
                     /locus_tag="IV334_002095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003550836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HAP8944152.1"
                     /translation="MRQNIILECVETGERLYLTSKNKRNNPERLELKKYSPKLRRRAI
                     FKEVK"
     gene            complement(10943..11320)
                     /locus_tag="IV334_002096"
     CDS             complement(10943..11320)
                     /locus_tag="IV334_002096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8944153.1"
                     /translation="MEIIYPPLVEEGLKYYLETTQQSLDKCTFYRSMVERGIITETGL
                     PTQQAIENGLVKDYYEDQGLSFDDFLRIYPIFEEYDEELFQCIDGYWEIPVDMKENLV
                     SQLESGELTFEDAQQIQAYLEDR"
     gene            complement(11352..11531)
                     /gene="rpmF"
                     /locus_tag="IV334_002097"
     CDS             complement(11352..11531)
                     /gene="rpmF"
                     /locus_tag="IV334_002097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018345355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HAP8944154.1"
                     /translation="MAVPARKTSKAKKRLRRTHQTVVKPEISFDEANGDYRRSHHVSL
                     KGYYNGKKVIKGASK"
     gene            complement(11546..11815)
                     /gene="rpsN"
                     /locus_tag="IV334_002098"
     CDS             complement(11546..11815)
                     /gene="rpsN"
                     /locus_tag="IV334_002098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HAP8944155.1"
                     /translation="MAKKSKIAKAKKQMAMIEKYADKRQELKAAGDRTALAKLPRDSN
                     PNRLRLRDQTDGRPRGYMRKFGMSRIKFRELAHQGLIPGVKKASW"
BASE COUNT         3847 a         2280 c         1858 g         4010 t
ORIGIN      
        1 tttgctcgat agttttactt agattttctg tatattcaat taacggttca tggaatagat
       61 cttctatttt tccagtaaaa aaataatgaa ctaaaatcag aatatctcga atgtctccta
      121 aagaatacaa agtcaaattt tgcaatgaat tatagagtcg ttctctatca gccattgcaa
      181 cattcaaatt gttcattttt agtttaaaaa aaactttatc aatattattt gctctaaatg
      241 gaccaaataa gtaatatctg gttttaaaca tgtatagaag aaacatctca ttaaaatcac
      301 cagtaaagaa atggaaatct ttagtttttt taatattatc agttagtaac tttttataat
      361 cataaaaaaa ataagtagtt ctatctgagc gatactcttt tataatactg aattcggaat
      421 taaaaaccac gataggtaac tttaaacaaa catgaagaga ctttaataag acttctttat
      481 ctgaaaacac ttttgtttct ctctttcctt tctacatcat taaatagtat tttatacgtt
      541 atctaaaata ctattcctct gtcaaaataa gctaacttac ttcattataa aatatccact
      601 tatcgctttc atgtatattc caccgtaaaa agtacatttt atcaccgaga actcatcatt
      661 tttcatcatg atgaatacta tttacatata tctagttcaa agacaataat tattatgatc
      721 cgttattatc taagcactat acagataata ggaatttttc gtttctaaat attccaattt
      781 gggaatccaa taaaaatttt cgttcaaata cgtgtctatt cgaactcttt tctagttgtt
      841 aattatgaag ggggaagaaa taaaaaatac aatctataca cgaataagaa tattatccaa
      901 aaggaggttt tagaaaagat ttattatacg taaaaagctc aagacatttt cttagccata
      961 aggacatgtt tgatatttaa gaagatatat gaattttctt ttctaagtat aaagataact
     1021 atccattatc aactaaaaaa acatttttga aaataaacat cagcagtcct atcaatgcat
     1081 gtgagcttat agaagttatt cgcagcgcag cataattgca ttagcttctt ttcgtgtttt
     1141 ttgcctcttt tccctttaaa tcttaatgga ggaggtgtgc aatatgtcag ataacgacga
     1201 acgcgtaaat gcccagttga ttcgattctt tgaaaaggtc attttgaaca ccgctaatac
     1261 ttattataaa aaacaagaag aaattcaaaa acatgaacaa cttgatcaac tcccaccata
     1321 tttgtttact aaagatacta taaaatttaa acaaccgatt aatgttttaa atgtgactct
     1381 ccttgttgaa gatataaaat tagcaaaaat ccttcctttg ttgaaagaac ctgaacaaat
     1441 ttttcttatc gaaaaattta tctttgataa aactgataaa gaaattggaa aggttctggg
     1501 gattacaaga cagggtgcga caaatttaaa acatcgactt tacaaaaaaa ttcaaaaaat
     1561 gctggtttaa tatctgcata ataactaatt ttgtatgctt ctttgctcgt tctcctcctt
     1621 cccaaagaaa tagattataa gtccatggga agaaggtaga tttatgaatt ctttaaaaga
     1681 agatttcatt ttagcaaagg ctggaaatga agaagctgtt gaggcaattc tcaaaagatt
     1741 tagctctttg attcataagc aatcctggag aactggaaaa tatgatcaag attgttatca
     1801 agaatgtatg cttgcaatat atctagctat ctcgaaattc gaaattaaag aataactcaa
     1861 ctaagggagt ctgtgacata agtaaaaaga tagtttcaaa tttcgaacaa taaatttgaa
     1921 actatcttct ttataattat aaagtatcga ttttacagcg ttctataatg attcatagaa
     1981 tctaaaacga atgattttaa ttatcaacgt tttttaattc gtttttagct taaaaataac
     2041 ttttgtcaca gtctcactcc taaaattact ttatttacgt atgaagtggg caggaggtcg
     2101 aatccagaac tgggctatgg tacttcaacg aggacttctc ctctagaatt gaaaagcatg
     2161 cgatttacct tccgtaacga cggtagattt attcaactat gtgtccaatc aaaatttgtc
     2221 tgatttcaaa aatagtcaag agaattttgg ttgttaaaaa ctcttatcta tcaacttcca
     2281 cagtttagtt gacaaaccct gatactaagt tgggctatga tacacttttc ttgactatca
     2341 gttacgttac cggaaggaat gaactctatt gagaaaaaca tatgatcgta aatttaaact
     2401 aaagatttca caggatatcc ttgagaaaaa agtaactacc aagaaaattg ctgaagaata
     2461 caatatctct cgtccaacaa tttcccgatg ggtttcagaa tatcgtcgat acgggaaaaa
     2521 tgcttttgct ggacaaggaa aaagattacc tgataaagca gatttctaca ttcttgaaca
     2581 ggaaaataaa cgactaaaag aggaagacga gtgttctacg aacacaaaga tcgatacgga
     2641 tctccaggga tcactcaaaa actttacgat gaaggaatcg aaatcaataa acgtgttgta
     2701 gctgttctta tgcgtgaaat gaatttatgc acaagaggat ttcatcgacg caaatcttct
     2761 catggtagag aaaaaacaat caaagaaatg gtaaaaaaat gttgaatcga taatttgaac
     2821 agaaccaaat tgatgctgtt tgggtgatgg atattactta cattccatgt aatgacggtc
     2881 gcctctattt gtccacctat attgacttag caactcgtat tctacgttac tatatggtag
     2941 attcacacat gaaaaaagag attgtcgtcc gtccattaca aatttattaa ggagaattgc
     3001 ctacggtaat tcattccgat agaggtagcc agcatcggtc ttttatttat caagagctgt
     3061 tgaacgacca ccacatcaca cattcagtga gccaaactgg gactcctgtt gacaatgcag
     3121 taattgaagc gtttcatcga tcaatcaaat gagaattaat cgactccaac aaatataaaa
     3181 gtaaaataga gatgaaggtg ttaatccaag attatttaga agactactac ccaaacaaaa
     3241 gaatccatac aaaatttatg atgactcctt gacaatttga agcgaagaaa ataagggatc
     3301 tataatatct agtgttggtg acgccttctg ttgccaatac tttttctttt ttctaataaa
     3361 cgcaaacaga caccttaaaa tcctttagaa taaagtcgac gaaaaccatt caaaggagtg
     3421 tttcaagatg tcctattcag agtttaccaa agagttacta gatattctag acctaaatct
     3481 tatctttcat gaagatgcct ttcgcaaaga gcgaatcaat gacgaaacat gttttgtttt
     3541 tgatggcaca ttgacctatc aaccggagga atgctttcat cgccactatc aaaatgaaca
     3601 aacgatcatt aaatagggct ggaaaaaagt atccatctta ttgaatgatg tcagtaacta
     3661 caagaccatt ctgcggatta acaaacagcg cttcaaatgt aaacactgtg gaaagacttt
     3721 tttagcagaa gattcagtta gtgatcgtcg ctgctccatt gctcgcagag tcaaacaagc
     3781 cattcttgag ctattaagcg aacctctttc tatgtctttg atcgcacgta tgaagcacat
     3841 ttctccgacc actgtcattc gaatacttcg cagccttcgg cctaaaacca tttctttgag
     3901 ccagctgtta ccagaagtgg tgtgtttcga tgaattcaaa tcagtgaaaa atgtctcggg
     3961 agccatgagc tttgtcatga tggacggcaa gacccatcaa ctgattgata ttgtggaaaa
     4021 tcggcaactc aatcctttac gcgactattt cttgcgctac cctataaaag tccgtgaaca
     4081 agtgcgtcta gtggtttcag atttctatac accctatcgc acattggtga aagagtgttt
     4141 tccgaatgcc cgaatcgtcg ccgatcgctt tcatattagc caacacattg gtcgggcttt
     4201 taccaaccac cgaattcaag taatgaagac ctttaaaaaa ggggatcgtc ggtcgaagca
     4261 cttaaagaaa tactggaaac tacttcaaaa gaatgcgtgg gaattgaaag gtcagcatcg
     4321 ctactggcgt ccgtcatttc gagatcatct gacagaagca gagatagtcg atcggctatt
     4381 atcttatgat gattccttaa agcgaggcta cgaagtctac caggattttc tttccgttat
     4441 cagaagacaa gatgttcctg aattcgtcgc gctcctaaaa gaagactaca aggagttgcc
     4501 agagcactac caaccagtgt tcacgacctt caagaaatac caaacagaaa tcaaacgagc
     4561 gttgcgcgtt ccctattcca acggccctat agaatgtttg aataaccata tcaaagtact
     4621 aaaacgaatt gcctatggct atagaaactt tcaaaactac cgagaacgaa tctttttata
     4681 tcgaggaaaa tattttaaga aaactaagaa tactacccaa ctgactaaag ccagaacaac
     4741 aacacggcta gacaagatag ctgtctagcc gtgtgagtat ctcgattatt ctattgaatt
     4801 tcttcaccaa cactcattga cgaagagcca aaataagtaa tctaaccgac tgttaactaa
     4861 accattgcag gtcccgttaa agatacttgt gcctgttgtc accgcttctt tttcttgtcc
     4921 acaagcgctt aacacactta ataaagcccc tgctattatt aataataata tctttttctt
     4981 attaaaaacc tcctcatcta tttccagatt agtaaatgta gtaatctgtg ctttcttttt
     5041 tctcaatact aataactaac ttatggggta aacaaatact cgtttgaccg ggttttgaga
     5101 tccatccagt tctaaccgcg atttgatcag gactattatc ttctttaact cgaattcttc
     5161 catctttaat ctcaacaata ttatattgtc ccttcgcagg atgataattc acgagtttat
     5221 gattgatctt atccaaatcg aagcgatcca tttccttgcc atcaattcga acgatggcat
     5281 atttgatagc atcttcagtt gtttcattct ccttttctat agagaataca aataagggaa
     5341 taaaagaggt aataatcaaa atgccaataa ttatgacatc gcctatcttg acaccagctt
     5401 ttcttaaaaa tttcattaac gatatctggc taaacatatt agactttttc catggaaatg
     5461 gtgaaatttt tcggaaatta tctgcattaa gagaatcagt taaacaaccc tcaagttgtt
     5521 ccttaataca ttgttgatcc atcttcatac caataaaaac tagtttatta atgcgttcac
     5581 caaacaacgg atgccaggct tccttcaatt cgggattttt ttgtataatc atttgacact
     5641 caattttagg taaactagct acccaataat ttaatgattc aatactttta tagctacccg
     5701 atgtttcaaa gatcgtcgaa aaatcactat gttgggcaaa ccaaagaaac ccttttgctc
     5761 ttgtaatatt gccaggaaaa tttttatccg taagatgggc aaatttttgt gcgtcaagga
     5821 gtaaccgatt tcgatagata aaacttgaga taccatattc ttctgtttca ggaatatgat
     5881 tttgatagcc aagctctaac tctttgaacc agcccactga ataggaagtc tcatctatat
     5941 taaaaagatt tgtatttaga attttcgata agtctacttc acctttaatt gtcctaatta
     6001 ttttggcatt ggactgtaac ttagcaatta gaccgttaat tttgtaaatc gtcttggttg
     6061 acactaagtc actattattt aaaagtaata tgttacaaca ctcgaattga tcaattaaca
     6121 agccacgaat atcctgttct tcttcaacta cattaccttc agcttttctt tcctgaatct
     6181 tttcacccgt ttcgaaatta tcccaaaaac gttttacatc cactactata ataatggcac
     6241 caatctctaa tcgttgggtc aaatcaatgt ctaaagggtt tttagctaat ataattgctt
     6301 gaataattgg aattggtttg ctgattcctg aagcttcaat aacaatttga ttcaagtcag
     6361 aaaattgcac taattgatct agttcaacaa tcaaatcttc gcgtaatgta caacaaatgc
     6421 atccattcgg catcgaaaca agtttttctc cagttctttt aaaacctttc ttgtcgacta
     6481 ataccccatc aacatttact tcccctatgt catttacaat gacagcaatt tcctttccgt
     6541 gatcaccgta cagaatatga tttagaagta tcgtttttcc tgaaccaaga tagccagtta
     6601 agattgtaat aggtattttt ttcataggga acctctttct ataccttgaa tttacacttt
     6661 aagtgcaaca cataaaaatg agtcataatc gatatccaac tagtataagt ttgtcgagaa
     6721 cactcctaga aggaaaataa ttatgagcca gtatcgtcat cttactatac tcgaacgaga
     6781 aagaatcttt ctgcttcacg aagaaggatt ttctattagt ataattggca acgacattca
     6841 gcatagcccg tttactattt tacatgaact gagaagaaac aagaaaaaaa ctattcacct
     6901 tccaaagcgg aaaaaaatta tgcttatcga agaaaaaaat gcggacgtta tcacattcta
     6961 aatgatccgt gagtttggtc aattgttcgt catttatttg tcgattttca gtggtcgcca
     7021 gaggaaatct cgaatcgttt gaagcatgaa catatggact tttcaattag ttttatcacg
     7081 atctaacgtg caatttgcaa tgatcttttc gatacagaac ccctctcaca cggcaataaa
     7141 ggcctgatcc gttcattgca ccatcgagga aaaacaagac atacaaaaaa tcatgtcgag
     7201 tctcgtggaa agatttgcct tactcattcg atccatggcc gtccaaaatc cgcagatgat
     7261 agaagtgatt ttggtcattg gaaagccgat attgtagctg gcaaaactgg tggtacttgt
     7321 ttagtgactt tgacagacag aaaatcatgc ttccttttag caggaaagaa ggtccccaga
     7381 aagaaagccg cttatgtgag cgagaaaatg attgaacttt taagcagtat tccaaatgag
     7441 gcggtcaaaa cgatcactcc agatagagga aaagaattct ccaatcattt cgcagttaca
     7501 gaagcattga ataatatgcc cttttatttt ccagatcctc atgcccttgg caacaatgaa
     7561 caaattaaaa tacgaataga ctcattcgag aatacttgcc aaaatcgaaa gagatagcta
     7621 cggtagataa ctcaacgata aatcagatga ttgaaaaact gaataacaga cctagaaagt
     7681 gtttagattg gagaacacct tatgaagtct tctttgataa agtgttgcgc ttgatttgac
     7741 aattcaagac ataataaaaa gcagatcaaa tccaaataag ggaaaaatga tctactctgg
     7801 tattatcaat taattggcca agctattatg ccgttttgct aaacgtctac ttcttccagt
     7861 attgaccttg ataaaacaac aatcaggatc ccgttttaat actctatgaa ctgtgtcaat
     7921 actaacatgt aaagttgcag caatatcagc taatgtactc ccatttaatc tcatttgctt
     7981 tgcaaatcga taacgccgat aagcatcaat attcttggaa tccatagaca ataatacttt
     8041 tcttaccatg tatttcctcc aaaaattgaa ttttaatgag ccatcatttc ttgagcaatt
     8101 tcatgtttag acaatgaagc tggatagaaa gtaggccaat tatgcaattc attatagagt
     8161 ttttcttgac tttctccacc aaagaaaata tggaaatgct ctgccttact tggtgcaatt
     8221 ccatgatcac taaattgaat atatttaaaa cgaccagcac ctgcatcatc agtttcaaaa
     8281 ttaaaacgaa caccacggtt tcctttttca taattcaaaa ttttgtatcc aacatatttg
     8341 tatgtgtatt gatgatgttc accgttgact acaaaatcaa tggtgttgtc tttaatgttg
     8401 atagtatcaa tatctgtctt atagccagta gtataataat ctttgtactc ggcagctgtc
     8461 atgtctttat tgatttttgc tttgtaatcg aaaacttcat ccaaaattcc atcttttaac
     8521 aacggataaa cagattgcca ttcgcctgta taatcactca attcgcgatc cttcacatcg
     8581 gcatctttaa agtacccatt agcaactgtc ttttcatcct tcgcttcttt ttctggaact
     8641 acctcttcac cagcaaccat agttgttttt tctaacgctt ttagattgtc ttccattact
     8701 gaaacataat cttcaccatt atccatttgt tcttttgtaa gactttcaag aggattcagt
     8761 acttccaatt ttatcccagc ttcgttggcc aaagtttttg caattttatc attagcattc
     8821 ttttcaaagt aaatataatt gatcttattt tttttaacat actcttttaa ttcagctaat
     8881 cgaccagaag atggctcttc ttcaggactt agtccagcaa taggaacttg tttcaatcca
     8941 taatccaaag ctaaatatcc aaaggctgcg tgttgcgtca caaaactttt ttgtttcgct
     9001 tctttaagac tagtggtata gtcagcatcc aaccgtttca gtttagtcaa atacttttca
     9061 gcatttgtct caaatacttt tgcttttttc ggatacaatt taactaactg atccttgata
     9121 tttgaaactt ccttaattgc cattttgggt gaaacccacg tatgaggatc tagttcatga
     9181 tggtgatctt cttctccgtg atcatgatct tcttcttcac tacctggcaa taaaatcatg
     9241 tcttttgttc cttctactac attaggccca gctttcttcc aactttcttt agcttttggt
     9301 acccatgatt ccatgttttc gttatgatat acaaaaacat cagcatcatg agcttttgcc
     9361 atatccttag ctgagggctc atagtcatga ggttctgatc ccgctggaac cattaaatcc
     9421 acgttcgctt catcgccgac gatgtttttg gtgaaatcat acattggata gaatgttgtt
     9481 acaatttcta attttccatc tttatcattc gatacttttg catcattagt gctggagcaa
     9541 gctcccaata atcctagagc tgctatccca ataagtccta cactgatttt cttcattctt
     9601 tttccttcct taattatgct aatttcttca ttttatttcg tttatgttct ttgataattt
     9661 ttaatgccct ttttctagtt ttaatattcg ggcttttaag tcgtcgatat gcactagata
     9721 tatccgtttt ctccattaga aattaccccc aatcactact tatcattatt tgctttccgc
     9781 cagtaattta tcaaaactct gtttgatcct ttcttcatcc aacatttttc cgattagaat
     9841 aatagtggtt tggtcttttt gtcgagtggt gtattgaaaa ttaacctgtt cgtttacctc
     9901 ttgcatggca actataaaat cctgctcttg taaagcaatc aatcctttaa aacgatacag
     9961 gctcccttgg ttggttaaaa tcagccagtc aatccattgg agtaaaaaat ctttttttat
    10021 gtcttgatct gaagttaaga gaattgattt aaactcatgt acttgatggt gttgatgata
    10081 gtatttgcca ttatagtgat tttctgatga atcactttca aaaactaagg gatgattaaa
    10141 ttttttcaaa ttataaaaat cctttttttg aatcgtttca ctatgtgtga acattcgaat
    10201 atcggataaa gggttaatac ttttgatttc tctctgtaat ttttttagtg cagataaatt
    10261 caagttttgt tttctagaca caaaaatgcg atctgccatg acaagttgct taagggcttg
    10321 tggttctctt tcaataagtt caaaactgtc actatcaata acagtcaata aactatcgat
    10381 atatactctt cctcttagtt gaggcgctgt taaaagtgtt tgaatgattg gttggggatc
    10441 tgctatacct gtagtttcaa tgatgatttg tgaaattggt tgttgatgtt cttctattgt
    10501 ttccagtaaa gaaatcaata ttgctaataa atcactgcga agactgcaac acatacatcc
    10561 attatttagt tgtaacaccc gttcctcgct atgaactaat aatttgtgat caatcccaac
    10621 ttcaccaaac tcattttcaa ttataataat ttcttccctg gggtagggtg agacttgcaa
    10681 ggcttgattg attagcgttg tttttccagc ccctaaaaac ccagaaacga ttgtaactgg
    10741 tatgcccatt ttgtcacctt tttaccactt agctatttca cttctttaaa aattgcccgt
    10801 ctgcgcaatt ttggcgaata cttttttaat tctaaccgtt cagggttatt ccgtttattt
    10861 ttgctggtga gatacagacg ttctccagtt tcgacacatt ccaaaataat attttgacgc
    10921 ataaatcttc ctccttcaat acttatcgat cttcaagata tgcttgaatt tgttgtgcat
    10981 cttcaaaagt taactcgcca gattccaatt gtgaaactaa attttctttc atatcaacag
    11041 gtatctccca ataaccatcg atacattgaa ataactcctc gtcatactct tcaaaaattg
    11101 gatagattct taaaaaatca tcaaatgata agccttggtc ctcatagtaa tctttgacta
    11161 atccattttc aattgcctgt tgggtcggta aacccgtttc agtaataatt cctctctcta
    11221 ccatcgaacg ataaaaagtg catttatcta gcgattgctg ggtcgtttca agataatact
    11281 ttaatccttc ttcaactaaa ggtgggtaaa taatttccat tttgattccc tcctaaaagt
    11341 tatatattaa actattttga agctccttta attactttct ttccattata gtaccctttt
    11401 aaagatacat gatggctacg tctataatca ccattcgctt cgtcaaatga aatttctggt
    11461 tttactacag tttgatgagt tcttcttaac cgtttcttcg ccttagatgt ttttcttgct
    11521 ggtactgcca tgatatttcc tccttttacc aacttgcttt ttttactcca ggaatcaacc
    11581 cttgatgggc aagttctcta aacttaatac gggacatacc gaactttcgc atataccctc
    11641 ttggacgacc atccgtctga tcacgaagcc tcagacgatt cggatttgaa tctcttggca
    11701 atttagcgag agcggtgcga tctcctgcgg cttttaattc ctgacgttta tctgcatact
    11761 tttcaatcat cgccatttgt ttctttgctt tagcaatttt cgactttttt gccatatacc
    11821 tttatactcc ttttattttg atttagacac ctcaaatcgt aatcgttacg ttttgacact
    11881 gatactttac caacaagagt tgctcttgtc aacaataaat aacatgaaat aaaactcata
    11941 tgaatagata actatttgaa aacgaattta tcggtacata gagataatca aatca
//
LOCUS       DACDKM010000085        11668 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20834_2016
            SAMN12640370-rid9891663.denovo.085, whole genome shotgun sequence.
ACCESSION   DACDKM010000085 DACDKM010000000
VERSION     DACDKM010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640370
            Sequence Read Archive: SRR10040891
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11668)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11668)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742835.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742835.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:04:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,012
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 29 of 75
            Pseudo Genes (incomplete)         :: 33 of 75
            Pseudo Genes (internal stop)      :: 19 of 75
            Pseudo Genes (multiple problems)  :: 5 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11668
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640370-rid9891663.denovo.085"
                     /isolate="E20834_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(38..217)
                     /locus_tag="IV334_002099"
     CDS             complement(38..217)
                     /locus_tag="IV334_002099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8944156.1"
                     /translation="MPIAIGNKRLPVTLDEKRQKELQQLKQKYGKSESRIMCIALDLL
                     IAQEKAGFEVPALKK"
     gene            complement(598..1305)
                     /locus_tag="IV334_002100"
     CDS             complement(598..1305)
                     /locus_tag="IV334_002100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8944157.1"
                     /translation="MNKVVYQLTTEDVSQFLTNKEHLNEVIRRIQKDFSCMEEMKYFI
                     QRVEEQLYMESETSQTKVTVSHNRLDVYKRRPDTLKKGAELLRLETEKYIMNVYLERS
                     ELVENFVSYTICRKHYYSESEYYSIFESMELMEEEMEELNSKDATVDDYYLVFTRFLT
                     CGTESIVLMGMDYELTVPEAFLREYVEEVDNPGDDWYTTLTDFLDTYTYDDTEEIQNE
                     LDRKRMPYQRFERKKGQ"
     gene            complement(1298..1735)
                     /locus_tag="IV334_002101"
     CDS             complement(1298..1735)
                     /locus_tag="IV334_002101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8944158.1"
                     /translation="MAQNYYDEFVKLPLDKMAQKMEDMTFLYHETRVPKKHYKEKLSV
                     AVEEMIESGVEMNLIATYYRTLEELKKQNGKWFFQALLCLEVGVKPSTIKPSEYQALE
                     LTYAKFAQIKKAKAMASDWLDTFENIEKNGAFYTIESEEKDNE"
     gene            complement(1750..2001)
                     /locus_tag="IV334_002102"
     CDS             complement(1750..2001)
                     /locus_tag="IV334_002102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8944159.1"
                     /translation="MKTQHKLLTVKEVVQILVDKEVISEKVAEALLIPRYWTIETPLV
                     EDAPTMLFEDLVKIAGYELHYQENMETYEIEYWLTKLEN"
     gene            complement(2001..2207)
                     /locus_tag="IV334_002103"
     CDS             complement(2001..2207)
                     /locus_tag="IV334_002103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase UvrA"
                     /protein_id="HAP8944160.1"
                     /translation="MRKKFDYWGVPFSELFPNYHAPHTVECDCGERAKCIKSYRLYQC
                     PTCGKKYTLSYGDYVLIDEKVKKR"
     gene            complement(2644..2904)
                     /locus_tag="IV334_002104"
     CDS             complement(2644..2904)
                     /locus_tag="IV334_002104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8944161.1"
                     /translation="MNKYVGLLDKIRVIKTQPLLVRFTLQTIHESINCVVADIEIIDK
                     LLIMDDGKYNIAVTGHFNKRNQLVIESMQIRNPDHFTRSMGI"
     gene            2979..3182
                     /locus_tag="IV334_002105"
     CDS             2979..3182
                     /locus_tag="IV334_002105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative holin-like toxin"
                     /protein_id="HAP8944162.1"
                     /translation="MIKTVKFVTVAIRLKVRWCVCAYFQNLTERRSLLPVETALGLMI
                     SFGSLIATIIFGILAVVNNNQKK"
     gene            complement(3323..3808)
                     /gene="ssb"
                     /locus_tag="IV334_002106"
     CDS             complement(3323..3808)
                     /gene="ssb"
                     /locus_tag="IV334_002106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016186236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HAP8944163.1"
                     /translation="MINNVTLVGRLTKDPDLRYTASGTAVATFTLAVNRNFTNQNGNR
                     EADFINCVIWRKPAETMATLAKKGSLIGVVGRIQTRTYDNQQGQRVYVTEVVADNFQL
                     LESKAATESRAHADQSSSSPSPTTFEQRDVATPNNNGLNASQNPFGGQSIDISDDDLP
                     F"
     gene            complement(3811..4710)
                     /locus_tag="IV334_002107"
     CDS             complement(3811..4710)
                     /locus_tag="IV334_002107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HAP8944164.1"
                     /translation="MTPTQFAAKTRNLAKSTGLNAQLVQRHFMMDRLIEQISESKYKN
                     DFILKGGFLLGSKYGIDRRTTIDIDTTFRNSKLTEEKLKSIFNDLTRKPTKEGIVFSI
                     QGMKETREADFYPGFQIKLVANLEKVRVPFKMDVTTGDSIYPEVDTHYHQLMFEDKKV
                     EIPAYPTEQIIAEKLSATFSFGTDNSRAKDYYDLFTIPKLENIDTKELYKSVRNTFTK
                     RGDTKPLSFYYEREMDKIRGNDYLEKQWEKYRSVNTFANSISFTDTVNEIDHLMKSVI
                     KIENQERKRQFKRSQQMNQEMER"
     gene            complement(4723..5316)
                     /locus_tag="IV334_002108"
     CDS             complement(4723..5316)
                     /locus_tag="IV334_002108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="abortive infection protein"
                     /protein_id="HAP8944165.1"
                     /translation="MQDKLKELLAIRDGNLSMKEARKFGIAATTVQRLVNQGKLIKVD
                     RGYYVEDGHGIDDLFWLQQRFSRGIFSHETTLDIYQLSTNMPKFIHLTFPHGYNVDRS
                     ITKEQQLQFHFVKKEVYELGKVEGTSFQGNPITMYDKERTLCDIWNPRYEFEYEMKLE
                     ALKEYMEDPLRDPSKLRDYMTKLHVEKEMKYHMQSLY"
     gene            complement(5557..7761)
                     /gene="topB"
                     /locus_tag="IV334_002109"
     CDS             complement(5557..7761)
                     /gene="topB"
                     /locus_tag="IV334_002109"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HAP8944166.1"
                     /translation="MKTVILAEKPSQAKAYADSFSKATRKDGYFEIQDRLFSGETVIT
                     YGFGHLVELDSPDMYDENWKQWSLEHLPIFPTHYHYHVPKDKKKQFKVVKQQLQSADT
                     IIIATDSDREGELIAWTIIQQAGADHGKIFKRLWINSLEKEAIYQGFQQLRDAEETYP
                     KFEEAQARQIADWLIGMNGSPLYSLLLQQKGIPGSFSLGRVQTPTLYMIYQLQEKIRN
                     FQKEPYFEGKAQVIAQNGAFDAKLDPNETQATQEAFEDYLKEKGVQLGKQPGTIHQVE
                     TEKKSAASPRLFSLSSLQSKMNQLMKASAKDTLEAMQGLYEGKYLSYPRTDTPYITEG
                     EYAYLLDHLDEYKHFLKAEAIPTPIHTPNSRYVNNKKVQEHYAIIPTKTVMTAAAFEQ
                     LSPLQQAIYEQVLKTTVAMFAEKYTYEETTILTQVQQLQLKTVGKVPVDLGWKKLFGK
                     ESDRKEKEEEPLLPKVTKGEAVTVDLQVLEKETKPPQPYTEGTLITAMKTAGKTVDSE
                     EAQSILKEVEGIGTEATRANIIETLKQKEYIKVEKNKLVVTNKGILLCQAVEKEPLLT
                     SAEMTAKWESYLLKIGEQKGTQATFLANIQKFVSHLLEVVPGQIQSTDFGSTLQEVKA
                     ASEKQEATRHLGVCPKCREQEVLLYQNVAACTSEACDFKLWTTIAKKKLTATQLKEII
                     QNGRTSQSVRGLKGQKGSFEATIVLKEDFTTGFEFSEKKKTNYKKRTRRTTK"
     gene            complement(7772..9232)
                     /locus_tag="IV334_002110"
     CDS             complement(7772..9232)
                     /locus_tag="IV334_002110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HAP8944167.1"
                     /translation="MTEKKMSLIDRCKQIDIVDFARNNGMAVVNKGRDYRLEDHDSFV
                     FDRRKQRFYWNSQNISGDIIELAKLFFIDKEIQDPKQQFKAATDFILKNEDKTERVEN
                     LHFETEKYKDHPVDYQPLTEKGRNYLKEERKLPEWLIDYAEKEGLIAELKPKHERQNF
                     LVRDDRLDHAVAFLWKDPQTNETVGASYQGTFIDYERFGERGTYKHIDKNSTANHGFN
                     LKIGDPKQLKFFESSIDLLSYAALNRDQLNDTWLVSMEGLKHHVISHYFGEAVSELRK
                     KQAFPQSIEICVDNDRAGHIFYEKEQLMGAVDPFTNQKVRCERGIANDWQVPKEYKVI
                     YEEVAKEEKITPEAIMAIHKTENNLQLTNQLVSAHKVNASFGQQLSVNDSIEAINLKD
                     ICREVAKELKACERVDGTYDFDRFYQEKGDINAQILFSYKAEQYYKGYKNHEHEFVPE
                     VKKDWNDQLKHEIYQQEIRKQKRAMLFQQGRQQERE"
     gene            complement(9293..11665)
                     /locus_tag="IV334_002111"
     CDS             complement(9293..11665)
                     /locus_tag="IV334_002111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8944168.1"
                     /translation="MAEEKEPLDNSRLGKSKRKLVRLQNELNEQIEKMFEHQRKTNGQ
                     PMNDKRNGHSWFRQQERLENKVHSLREEIKQQEKQVEKLERQEELKEMGYNKYGGLDM
                     TIENIPRIKEEIERFEKGESTFSAATIRKYQRKLETLEQLKERSEKGKENLLPEVQAI
                     IDSGRVTQWKKNPTIYFLKDYRKVALELNENGGFEESKKYQATSEEEKAAVRELLEEA
                     APTDSLKEKENDHFYRVEFNENDPEKGLKSYGGEIVTLELIDQLKTLDNQYAAEAGYY
                     KFYFEEVKNGEVVDQIRMDLGDGVNSNQPIYLELAKLCEQVPEKEQKQTVKNEPVSKE
                     QMQSKETKTKQKGYQKRTAEEIKQEVKSLSQQALDAVKKHTHSPEDVKELLNFMSRFP
                     ERSFRNQLLIEEQFPGATACLGRAALKKEGIYIKKGEQGNKIFVRKTVKGFYEKGRGF
                     VRETEATPEDKKRIESGQIEVIKKPYYTIEKVFDITQTQLKPEDYPKIFPNRVFDFQL
                     DKKGQSELQAGIQAVANNIGIQIRDMTESEVYQRELGQARGAYVRNEFTGEEEIVMNT
                     RNTPTQHLATSIHELAHARMHKFSELDTATKELQAEMTSYIVCKHFGMDTSEKAIPYI
                     AAWTKNGQTLDDKEAAERGKIMNDVSRVANEFIQTISNEINQYREREPEIQPAEVKQE
                     HKNTVETAEQKNIVPVGSLWIDKKDGKVMEEDTGRTLHLKDAAFIEDPQHNQVILKGK
                     SYGKEIQTALPSEEFALGNSFRFSLETVSEVAKRKNEIYYEQQLSQKERV"
BASE COUNT         2892 a         2432 c         1958 g         4386 t
ORIGIN      
        1 gtgctatcac ttttagtttt taggataaaa agtgacgtca ctttttgagt gctggtacct
       61 caaatcctgc tttttcttgg gcaatcaata aatctaacgc aatacacata atcctggatt
      121 cacttttgcc gtacttctgc tttagttgct gcaattcttt ttgtctcttt tcatccaacg
      181 tcacgggtaa ccgtttattt cctattgcaa ttggcattag tactctctcc tttcatcgaa
      241 ataaccctat taaaaatatg gctgcaatca acttgaatat tagactcaca agggttaaca
      301 caaagcgcca aaagatcaaa caaaatacca gtattcctaa gaatactacg aagtttaaat
      361 gtagagctgc accatgacgc ataagtatct cgccaacaaa cagtacgaac aatagaggaa
      421 tgattttatt catatggatc tccttctttc tcttaggtta tcgggctttg cccgcttatt
      481 tcgcaccacc taagcggcgg gggcggttgg atcgattggt gcgaaataag aaccattccc
      541 ttaatttcaa ggttttttgt tcttatttca cacatcctca cttttcggta taaatggtta
      601 ttgacctttt ttcctttcaa atcgttgata tggcattcgt ttacggtcaa gttcattttg
      661 gatttcttct gtatcgtcgt aagtgtaggt gtctaaaaaa tcagttaaag tcgtgtacca
      721 gtcgtcgcct gggttatcta cttcttctac atattccctc aaaaatgctt cgggtacagt
      781 gagttcataa tccattccca ttaacacgat agattccgtc ccacacgtca aaaatctagt
      841 aaatacaagg taataatcgt ctaccgtcgc atctttgctg tttagctctt ccatttcttc
      901 ttccattaat tccatgctct caaaaattga ataatattct gattctgagt aatagtgttt
      961 cctgcaaatc gtgtagctga caaagttttc gaccagctca ctcctttcta ggtaaacatt
     1021 catgatatat ttttctgttt ctagtcgtag aagctccgct ccttttttta atgtgtcagg
     1081 acgacgttta tacacgtcta aacgattatg gctgactgta accttcgttt gactcgtttc
     1141 actttccata tacaactgtt cttccactct ttgaataaag tacttcattt cttccataca
     1201 actaaagtct ttttgaatcc gtctgattac ttcattcaag tgttctttgt ttgttaaaaa
     1261 ttgcgaaaca tcttctgttg ttagctgata aaccacctta ttcattatct ttctcctccg
     1321 attcaatcgt ataaaatgca ccattttttt cgatattttc aaaagtatct agccagtcgc
     1381 tagccattgc tttagctttc ttaatctgtg cgaattttgc ataagtcagt tctaaggctt
     1441 gatattcgga aggtttgata gtgcttggtt tgacacctac ttccaagcat agtaaggctt
     1501 ggaaaaacca ttttccattt tgttttttca gttcttccag tgttcgatag tacgtcgcaa
     1561 tgagattcat ttcgacacct gattcaatca tttcttccac tgcgactgac aatttttcct
     1621 tgtaatgttt tttgggtaca cgtgtttcat ggtataggaa cgtcatatcc tccatttttt
     1681 gtgccatttt atccaaaggt aatttgacaa attcgtcata gtaattttga gccatatttt
     1741 ttctcctttt tagttctcta atttggttaa ccaatattcg atttcataag tttccatgtt
     1801 ttcttgataa tgcagttcat atcctgcaat cttcactagg tcttcaaata gcatagtcgg
     1861 tgcatcctct actaaagggg tttctatcgt ccagtaccgc ggaataagta aagcttctgc
     1921 tactttttca gaaatgactt ctttgtccac caaaatttga acgacttctt ttacagtcaa
     1981 taatttgtgt tgtgttttca tcaccgtttc tttactttct catcaatcaa aacataatcg
     2041 ccataagaca aggtatattt tttcccgcat gttggacatt gatacaaacg atagcttttg
     2101 atacatttcg ctctttctcc acaatcgcat tcgacagtat gtggtgcatg atagtttggg
     2161 aataattcgc tgaagggaac accccaataa tcaaatttct ttcgcatgat tgattctgcc
     2221 tcctcttagg aaaggtttta tttcctttcg ttagttcttt ctttattgat ttgttggcca
     2281 gaatgaatca acgaaaagcg acaaggacaa gggcgcgcag cgtgccattt acccttgtcc
     2341 ttgtcgcttt ttggtgataa aaggcaactt gttgccggtc tggcaaatca ataaagagat
     2401 agcgaacagg aacgaacctt ttggtgagag gaagtttttt ctattttgtc ttgctttgca
     2461 agacaccata tcttagtgaa cgtgcaacgt tcacttcctt tcttctcgaa cggcggtggc
     2521 ggttggagtg agagaaaggt ttttcaattg agaaggcact tgtgtcttcc taaaaaggtc
     2581 ttttcctgtt tctatgtaaa acaaaaagag ctgcaatcct catttaggac tgcagctctt
     2641 cttttatatg cccatcgatc gcgtaaaatg atcggggttt ctaatttgca tcgattcaat
     2701 gactaattga ttccgtttat tgaaatgacc tgtgacggca atattgtatt ttccatcatc
     2761 cataattaac aatttatcga taatttcaat atctgcaact acacaattaa tcgattcatg
     2821 aattgtttgt aaagtaaagc gaaccaataa tggttgtgtt tttattactc ggattttatc
     2881 aagcaaacca acatatttgt tcatcacgat cacctcgcta aaattatagc aaaaacagct
     2941 aaaattgaga atttttattc gattttagat atgctataat gatcaagaca gtaaagtttg
     3001 tgacggtggc tatccgattg aaagtgaggt ggtgcgtatg tgcgtacttc caaaatctta
     3061 cagaaaggag aagccttttg cctgttgaaa cggcgctagg attaatgatt agtttcggtt
     3121 ctttgatcgc aacaatcatt tttggaatct tagcagttgt aaacaacaat caaaaaaaat
     3181 aaccgtcagc tttagccggt cgacgattat ttttcaaaaa taatattaag ctggtcaccg
     3241 tcttaaacgg tgctgttagg ggagttgtta gcgcaactcc tcttttcgta tccattataa
     3301 caactttatt gatcttttac aatcagaatg gtaaatcatc atcactaata tcgattgatt
     3361 gtccgccaaa tggattttgt gaagcgttca atccattgtt gttaggcgta gctacatccc
     3421 gttgttcaaa ggtggtaggg cttggactgc tggacgattg atcagcgtgc gcacggcttt
     3481 ctgtcgctgc tttactttct aatagttgga aattatcggc cacaacttct gtcacataca
     3541 cacgttgtcc ttgttggtta tcgtaggttc gggtttgaat ccgtccaaca acgccgatca
     3601 aactgccctt tttagctaat gtcgccatcg tttctgctgg tttgcgccaa atcacacagt
     3661 taataaaatc agcttctcga ttgccgtttt gattcgtgaa atttcggttg acagctaggg
     3721 taaaagtcgc tactgcagta ccactcgcgg tataacgtaa atctggatct ttggttaagc
     3781 gtcctactaa agttacatta ttgatcatct ttatctctcc atttcttgat tcatttgttg
     3841 tgatctttta aactgccgtt ttctttcttg attttcaatt ttgatcacac ttttcattag
     3901 gtgatcaatt tcgttgactg tatctgtaaa tgaaatggaa ttcgcaaacg tgttaactga
     3961 gcgatatttt tcccactgct tttccaaata gtcatttcct ctgatcttat ccatttctct
     4021 ttcataataa aaggacaacg gttttgtatc cccacgtttt gtaaatgtat ttcgaacact
     4081 tttgtataac tctttcgtat cgatattttc tagtttcgga atggtaaata agtcgtagta
     4141 atcttttgct ctcgaattat ctgtgccaaa gctgaacgta gcacttagct tttctgcaat
     4201 aatttgttct gttggataag ctggaatctc cactttttta tcttcaaaca ttaattggtg
     4261 atagtgtgta tctacttcag gataaataga atctcctgtt gtcacgtcca ttttaaacgg
     4321 tacacgtact ttctctaaat tagccaccaa tttaatttga aatccaggat aaaaatcggc
     4381 ttctcttgtt tccttcattc cttgaataga gaaaacaatg ccttcttttg tgggttttct
     4441 agttaaatca ttaaaaatgc tcttcaattt ttcttcagtt aatttgctat ttctaaatgt
     4501 cgtatcgata tcaattgttg tccttctatc gattccatat tttgatccta ataaaaagcc
     4561 gcctttcaaa atgaagtcat ttttgtactt tgattcagaa atttgttcaa ttagacgatc
     4621 catcataaaa tgccgttgca ctaactgtgc atttaatcct gtactctttg ctaaatttct
     4681 tgtttttgct gcaaattgtg tcggtgtcat ctttttcctc catcaatata agctttgcat
     4741 gtggtatttc atttcttttt ctacatgtag ttttgtcatg taatctctta atttacttgg
     4801 atctctcaat gggtcttcca tgtactcttt taatgcttct aatttcattt catattcaaa
     4861 ttcatatctc ggattccaaa tatcacaaag tgtacgctct ttatcataca tagtaatagg
     4921 atttccttga aaggaagttc cctctacttt tcctagttca taaacttctt tcttcacgaa
     4981 atgaaattgt aactgctgct cttttgtaat tgaacgatca acgttatagc catggggaaa
     5041 agttaagtga atgaatttcg gcatattcgt agaaagctgg taaatatcta aagtcgtttc
     5101 atgagagaag attcccctgg aaaatcgttg ctgaagccaa aataaatcat ctattccatg
     5161 accatcctct acataataac cacgatcaac tttgatcaat tttccttgat tcaccaatct
     5221 ttgtacagtg gttgctgcga ttccaaattt cctggcttcc ttcatagata aatttccatc
     5281 gcgaatagcc aacaattctt tcagtttatc ttgcatttac ttcccctccc ttcgttacaa
     5341 atacactaca tcaaatatat catagtagtt tatttgtaac aagattaacg tctatcgatc
     5401 taattcgatt tctttttgaa tgcgctgctg gttctttaat tgttgcagtc ttcttttatt
     5461 tgcacgtaat ttctcttgtt ctaaacgaat ttttgctttt tgattttcga tttcttgttg
     5521 tttgtgtgtt gtatttcgca taggataacc ccttcattat tttgtagtgc ggcgtgttct
     5581 tttcttatag ttcgtttttt tcttttcaga aaattcaaag cctgttgtaa agtcttcttt
     5641 taaaacgatc gttgcttcaa acgaaccttt ctgacctttt agtcctctga ctgattggct
     5701 tgttcggccg ttttgaatga tctctttcag ttgtgtggct gtgagtttct ttttggcaat
     5761 cgttgtccat aatttaaaat cacaggcttc agacgtacat gccgccacat tttgataaag
     5821 caagacttct tgttcccgac attttggaca aacacctaaa tgacgcgttg cttcctgttt
     5881 ttcactggct gctttcacct cttgtaacgt tgatccaaaa tctgttgact ggatttgtcc
     5941 cggtacgact tccaacaagt gagagacaaa tttttggata ttagctaaga aagtagcttg
     6001 tgttcctttc tgctcaccaa tctttaataa gtaactttcc catttggcgg tcatttcggc
     6061 acttgttagc agcggctctt tttccacggc ttggcaaagt aaaatccctt tatttgtcac
     6121 cacaagctta tttttttcta ccttgatata ttccttttgt ttcaaagttt caataatatt
     6181 tgctcgtgtt gcttctgtac cgattccttc tacttctttc aatatggatt gtgcttcttc
     6241 gctatccacg gtttttccag ctgttttcat cgctgtgatc aacgtacctt cggtataagg
     6301 ttgtggtggc ttcgtttctt tttcaaggac ttgtagatct accgtcaccg cttctccttt
     6361 tgtcacttta ggaagtagcg gttcttcttc cttttctttt ctgtcgctct ctttgccaaa
     6421 cagctttttc caacctagat ctactggtac ttttccgact gtctttagct gaagctgctg
     6481 aacttgcgtc aagatcgttg tttcttcata ggtatacttt tccgcaaaca tggccaccgt
     6541 tgtttttaac acttgttcat aaatagcctg ctgcagtgga cttaattgtt caaaagccgc
     6601 tgccgtcatg accgtctttg taggaatgat cgcgtaatgt tcctggactt tcttattatt
     6661 cacatatcga ctattcggtg tatgaatagg agtaggaatg gcttcggcct ttaaaaagtg
     6721 tttgtattcg tctaagtgat ccaacagata cgcatactcc ccttctgtaa tataaggggt
     6781 atctgttcgt gggtagctta gatatttccc ttcatacaac ccttgcatgg cttccaaagt
     6841 atctttggca ctagctttca tgagctgatt cattttagat tgaaggctgg atagagaaaa
     6901 aagacgtgga cttgccgcac tttttttctc ggtttcaact tggtggatcg tacctggctg
     6961 ttttcccaat tgaaccccct tttcttttaa ataatcttca aatgcttctt gcgtcgcttg
     7021 ggtttcattc ggatcaagct tcgcatcaaa tgcgccattt tgagcgatta cttgggcttt
     7081 tccttcaaaa tatggttctt tttggaagtt tctaatcttt tcctgtaatt gataaatcat
     7141 gtaaagcgtt ggggtttgga ctctgcctaa ggaaaaactt cctggtatgc ctttttgctg
     7201 caagagtaaa ctatacagcg ggctaccgtt catgccgatc aaccaatcgg cgatctggcg
     7261 tgcttgtgct tcttcaaatt ttgggtaggt ctcttctgca tctcgtaact gctggaatcc
     7321 ctgataaatg gcttcttttt ctaaggagtt gatccaaagt cttttaaata tctttccatg
     7381 atcggcgcct gcttgttgaa tgatcgtcca agctatcagt tccccttcac gatcgctatc
     7441 tgtcgcaata ataatggtgt cggccgattg aagttgctgt ttcaccacct tgaattgctt
     7501 tttcttatct ttaggaacat gatagtgata atgtgtaggg aaaataggca aatgctctaa
     7561 tgaccattgt ttccaatttt catcgtacat atcgggcgaa tctaactcga ctaaatgacc
     7621 aaaaccatac gtgatcacag tttctcctga aaacaatcga tcttggatct caaaataccc
     7681 atcttttcgg gtagctttag aaaagctatc ggcataagcc ttcgcttgtg acggtttttc
     7741 tgctaaaata acggttttca tcatgacgat ttcactccct ttcttgctgt ctaccttgtt
     7801 gaaacaacat ggcacgtttt tgctttctga tctcttgttg gtagatttca tgcttcaatt
     7861 gatcattcca atcctttttt acttcaggta caaattcatg ttcatgattt ttatagcctt
     7921 tgtagtattg ttctgcttta taagagaaaa gaatctgagc attaatatcc cctttttctt
     7981 gataaaaacg atcaaaatcg tacgtaccat ccacgcgttc acaagctttt aattcttttg
     8041 cgacttctct acaaatatct ttcaaattaa tagcttctat tgaatcgtta acagaaagct
     8101 gctgaccaaa agacgcattt actttgtgag cggaaaccaa ttggttcgtt aattgcagat
     8161 tgttttctgt cttgtgtatg gccataatag cttctggtgt gatcttttct tcttttgcga
     8221 cttcttcata aatgactttg tattcttttg gcacttgcca atcattagcg attcctcgtt
     8281 cacaacgtac tttctgattc gtaaatggat caactgcgcc cattaattgt tctttttcgt
     8341 agaaaatatg tcctgcacgg tcattatcca cacaaatctc gatggattga ggaaaggctt
     8401 gtttctttct caattcagat actgcctcac caaaataatg actgatcaca tggtgtttta
     8461 acccttccat cgaaactaac cacgtatcgt ttaactgatc acgatttagt gcagcatagc
     8521 taagtaaatc aatactactc tcaaaaaatt ttaactgctt agggtcaccg atctttaggt
     8581 taaagccatg attcgctgtc gaatttttat ctatatgctt atatgtgcct ctctcaccaa
     8641 aacgttcata atcaatgaac gttccttgat aactcgcccc tactgtctca tttgtctgtg
     8701 gatctttcca taaaaaggcg accgcatgat ctaaacgatc gtctcgaact aagaaatttt
     8761 gccgttcatg ttttggcttt agttcggcaa tcaacccttc tttttctgca taatcaatca
     8821 gccactctgg tagttttcgt tcttccttca gataattcct tcctttttct gtgagtggtt
     8881 gataatcaac ggggtgatct ttgtactttt ctgtctcaaa atgaagattt tccactcgtt
     8941 ctgttttgtc ttcattcttt agaataaaat cagtggctgc tttaaattgt tgcttagggt
     9001 cttggatctc tttgtctatg aaaaaaagtt tagctaattc aataatgtcg ccactaatat
     9061 tttgactgtt ccaataaaaa cgttgttttc ttcgatcaaa aacaaatgaa tcatggtctt
     9121 ctaagcgata gtctcgtcct ttattgacta cagccattcc attgtttcga gcaaaatcca
     9181 ctatatcgat ctgtttacat cgatcaataa gactcatttt tttctcggtc attttaaaac
     9241 ctcccaaaag aaaaaagact atccgttttt cagatagtct tttttacttg ttttatacac
     9301 gttctttttg tgacaattgt tgttcataat agatttcatt ttttcgtttt gccacttctg
     9361 acactgtctc aagtgaaaat cggaatgaat ttcctagcgc aaattcttct gaaggtaacg
     9421 ccgtttggat ctcttttcca taactttttc cttttaaaat tacttgatta tgctgtggat
     9481 cttcaataaa cgccgcatcc ttcaaatgta acgttcgacc cgtatcttct tccattactt
     9541 tcccgtcttt cttgtcgatc caaagtgaac caacaggaac aatattcttt tgttctgccg
     9601 tttctacagt atttttatgc tcctgcttca cttctgctgg ttggatctct ggttctcttt
     9661 ctctgtattg attgatttca ttagaaatgg tttgaatgaa ttcatttgcg acacggctga
     9721 catcattcat gattttccca cgttccgcag cttccttatc atccaacgtc tgaccattct
     9781 tcgtccatgc tgcaatataa ggaatagctt tctccgaagt gtccattcca aaatgtttgc
     9841 acacaatata agaagtcatt tctgcctgca attctttagt tgcggtatct aattctgaaa
     9901 atttgtgcat tcttgcatgg gctaattcat gaatacttgt agctaaatgc tgcgtaggag
     9961 tatttcgagt attcatgacg atttcttctt cgccagtaaa ttcgttacga acatatgccc
    10021 cccgtgcttg tcctaattct ctttgataga cttcactttc tgtcatatct cgaatttgta
    10081 ttccaatatt gttggctacc gcttgaatcc cagcttgaag ttctgactgt ccttttttat
    10141 ccaattgaaa atcaaagaca cggttcggaa agattttcgg atagtcttca ggttttaact
    10201 gtgtttgtgt gatatcaaac actttttcaa tcgtgtagta aggttttttg atcacttcga
    10261 tttgaccact ttcgattcgt tttttatctt ctggtgtagc ttctgtttct cgaacaaagc
    10321 cacgtccttt ctcataaaat cctttgaccg tttttctgac aaagatcttg ttcccttgtt
    10381 cccctttttt gatatagatt ccttcttttt tcaacgcagc acgacctaaa caagccgttg
    10441 cacctggaaa ttgttcctca atcaataatt gatttctaaa ggatcgttct ggaaaacgag
    10501 acataaaatt caaaagttct ttcacgtctt caggagaatg ggtatgcttt ttcaccgcat
    10561 ccagtgcctg ttgcgaaagg gatttgactt cttgcttaat ctcttctgcc gttcgttttt
    10621 gatagccctt ttgttttgtt tttgtttctt tcgactgcat ttgttctttc gaaacaggtt
    10681 catttttgac cgtttgtttt tgttcctttt caggtacttg ttcgcacaat tttgctaact
    10741 ctaaatagat gggttgatta ctgttgacgc catcccctag atccattcgg atctgatcga
    10801 ctacttctcc gttcttcact tcttcaaaat agaatttata ataacctgct tctgcggcgt
    10861 attggttatc caacgttttg agttgatcga tcaattctaa tgttacgatc tccccaccat
    10921 aacttttcaa tcctttttct ggatcatttt cgttgaattc tactcgatag aaatgatcgt
    10981 tttctttctc cttcaaagaa tcagttggcg cagcctcttc aagtaattca cgaacagctg
    11041 ctttttcctc ttccgacgtg gcttggtatt tttttgattc ctcaaatccc ccgttttcat
    11101 tcagttctaa agcaactttt cgatagtctt tcaaaaaata aatggttgga ttttttttcc
    11161 attgcgtcac tcgtccgctg tcaataattg cttgtacttc aggaagaaga ttttcttttc
    11221 ctttttctga tctttctttc agctgttcta atgtttccaa tttccgttgg tattttcgga
    11281 tcgtagcggc tgaaaaagtc gattcacctt tttcaaagcg ttcgatttct tctttgattc
    11341 ttggaatatt ttcgattgtc atatccaggc cgccgtactt gttatagccc atttccttta
    11401 gctcttcttg tctttcaagt ttttctacct gtttttcttg ttgcttgatc tcttctctca
    11461 gactatgcac tttgttttct aaacgttctt gttgcctaaa ccaagaatgc ccatttctct
    11521 tatcgttcat tggctgaccg ttggtctttc tctggtgttc aaacatcttc tcaatttgtt
    11581 cgtttaattc attttgcaat cgtactaatt ttcttttgga ttttcccaag cgactattgt
    11641 ctagtggttc tttttcttct gccatgtc
//
LOCUS       DACDKM010000086        11438 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20834_2016
            SAMN12640370-rid9891663.denovo.086, whole genome shotgun sequence.
ACCESSION   DACDKM010000086 DACDKM010000000
VERSION     DACDKM010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640370
            Sequence Read Archive: SRR10040891
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11438)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11438)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742835.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742835.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:04:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,012
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 29 of 75
            Pseudo Genes (incomplete)         :: 33 of 75
            Pseudo Genes (internal stop)      :: 19 of 75
            Pseudo Genes (multiple problems)  :: 5 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11438
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640370-rid9891663.denovo.086"
                     /isolate="E20834_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            106..1620
                     /locus_tag="IV334_002112"
     CDS             106..1620
                     /locus_tag="IV334_002112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavocytochrome c"
                     /protein_id="HAP8944169.1"
                     /translation="MKKLVAGLLTAVALLAMSACGADQKNTAQTSSSTKESAEVSSGA
                     SEQVYTDPKEMKESYDVVIIGAGGAGMAAAIEAKDAGLNPVILEKMPVAGGNTSKSSS
                     GMNASETKFQKEEGIKDSNDAFYEETLKGGGGTNDKEMLRYFVDHSADAVDWLDQNGI
                     KLDNLTITGGMSEKRTHRPSDGSAIGGYLVEGLLNNVHEREIPIFVNADVTDIKKNGE
                     TVNEVTVHVQGEEPKEVTGKAIVVTTGGFGASKEFIEKYRPDLADYITTNQEGSTGDG
                     ITMIEKLGGQTVDMDKIQIHPTVQQDEGVLIGEVVRGEGAILVDQEGKRFVNEMDTRD
                     KVSAAITALPEKSAYLIFDQGVRDRATAIEFYDQKGYVTTGKTMEELAEKIDLPKETL
                     AKTVDGWNTDVAEKKDAQFNRATAMEHDLSSPNYYAIKIAPGIHYTMGGVKINTNTEV
                     LDKENQPIRGLYAAGEVTGGLHGNNRIGGNSVAEIIIFGRQAGIQAARFASENE"
     gene            complement(2023..3006)
                     /locus_tag="IV334_002113"
     CDS             complement(2023..3006)
                     /locus_tag="IV334_002113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-quinone oxidoreductase"
                     /protein_id="HAP8944170.1"
                     /translation="MRAISIKHPGSVDVLEQIEVPVPKPKQGELLIKVHTAAVNRTDI
                     MKRESVSLKPPYPILGVEVAGEVVENKSDISTFTPGTKVYGLVNLGGYAEYAVMPADR
                     AILLPDTLDYVSAAGISEVFLTAYQTLYWLGKLQEGETVLIHAGASGVGTAAIQLARQ
                     LSHAKIIVTAGSEEKLAFCKELGADELINYKKQDFQEEVRKITEGRGVDVILDFIGAS
                     YWEKNLASIAVDGRWVLIGMLGGTIVPEIDLGVLIGKRVQLLGTLLTPRSDQYKAELA
                     QTFMQKAGPFLENGKIRPIIDRTFQFSAVKQAHEYMEANKNIGKIILKITE"
     gene            complement(3011..3658)
                     /locus_tag="IV334_002114"
     CDS             complement(3011..3658)
                     /locus_tag="IV334_002114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAP8944171.1"
                     /translation="MKIFVVGANGQIGRHLIKDLAPSSHEIFAGVRDVASQTLVKKEN
                     VSYVSFDLTWSVEKMTEAFKGIDVLIFAAGSQGKNLLQVDLDGAIKTVIAAENAHVSR
                     YLMVSAVYADEPAKWPESMTDYYITKHYADEWLKRTNLDFVILQPVTLTNDEEVTSIQ
                     LTKPNEKASKTITRSTVAAVLAALVEETDISRTTLVLSEGSKELNTAFQEWAKEE"
     gene            complement(3743..4375)
                     /locus_tag="IV334_002115"
     CDS             complement(3743..4375)
                     /locus_tag="IV334_002115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HAP8944172.1"
                     /translation="MKNTKNFTLTAMFLAILILLAVTPLGFIPIGPINATTMHIPVII
                     ASIVLGPRLGAFLGGTFGLISMIRSTVIQTPLSFVFSPFIPVIGTEHGSWKALLIAFI
                     PRILIGVVPYFVYKGLRKLMKQKADSVSLFLAGLSGSLTNTILVMNMIYFLFQQDYAK
                     VIGTNLNAVYSAILAVIFTSGIPEGIVAGLATAAVCNVLLRYFKHPSLTN"
     gene            complement(4391..4942)
                     /gene="coaC"
                     /locus_tag="IV334_002116"
     CDS             complement(4391..4942)
                     /gene="coaC"
                     /locus_tag="IV334_002116"
                     /EC_number="4.1.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenoylcysteine decarboxylase"
                     /protein_id="HAP8944173.1"
                     /translation="MKKILLGVTGSISAYKSADLTSQFVKLGAQVDVIMSKSSTHFIT
                     PLTLQSLSKRAVHTDVMQESNPQVINHIELAKQADLFLIAPATANIIGKLANGIADDL
                     LSTVAMALPVGTPKLIAPAMNTNMYQNPITQRNLETLRSVGYQEIEPRESLLACGDFG
                     KGALADIQTIIEQVMLTLNERNA"
     gene            complement(4939..5676)
                     /locus_tag="IV334_002117"
     CDS             complement(4939..5676)
                     /locus_tag="IV334_002117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014386989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenate--cysteine ligase"
                     /protein_id="HAP8944174.1"
                     /translation="MKILITAGGTTEKIDQVRAITNHSTGRLGQALADYLAANPDTTI
                     DYVTTKQALKPKRHSNITIYTIESALDLFLQLEELTKKEHYDAIIHSMAVSDFTPAFS
                     FSEEQLAKNLPTSSTQEELDNWFAENEQTDTTVSKISSNTEHLVLVLKKTPKIISYLR
                     EWQPKAKIIGFKLLVDVPKESLLAVAKNSLINNKTDFIFANDLTEIHGETHHGYLLSK
                     DGTVEEAQSKSEIAALITEKIRLEESK"
     gene            complement(5673..6662)
                     /locus_tag="IV334_002118"
     CDS             complement(5673..6662)
                     /locus_tag="IV334_002118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HAP8944175.1"
                     /translation="MNAFRSFQVIKEPVFHTAISDLPFTELAENEVLVKIAYSDVNYK
                     DALAMSESGQVIRTYPMTPGIDLSGTVVQSNNPRFSKEDLVLATGFGLGVTHPGGYSQ
                     YQKVPGDWLVPLPKNMTLRQAMILGTAGFTAMLCVNALIRQGMTSDKKVVVTGASGGV
                     GSTAIALLHKLGFQSIIAFSRKEESVTWLKSLGALQVVRPDEFLPETTKALGKQQIDY
                     VIDTVGGEQLSSLLPLISYNGAVALCGNAGGIKLNATVLPFILRNIQLIGIDSVNVPI
                     DQRLSLWQQMADLQIADELVVQEITLDQLPETASKLLAGTHQGRTLVNVGDHK"
     gene            6920..7462
                     /locus_tag="IV334_002119"
     CDS             6920..7462
                     /locus_tag="IV334_002119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP8944176.1"
                     /translation="MEIGEKLRNLRIQKNLTQEELGERTDLSKGYISQLERDLSSPSM
                     ETFFSILEVLGVTPEEFFHQEAANLQVVYSKEDHTVYFDEENGYELEWLVADSNEKEM
                     EPVLLTFEGAGEYKTFEPSLSETFIFVLEGELELSLGETVYVAKAGQSIYYQATKQHQ
                     LRNHLKKRTKVLIVATQSYL"
     gene            7479..8564
                     /locus_tag="IV334_002120"
     CDS             7479..8564
                     /locus_tag="IV334_002120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016182253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP8944177.1"
                     /translation="MGKPIISFDQVVKRYDDETVLKKVSFEIEQGKFYTLLGPSGCGK
                     TTILRIIAGFTEATEGDIYFEGKRLNDLPANKRQVNTVFQDYALFPHMNVFDNVAFGL
                     KIKKMSKSDIEKKVKDALRMVQLPGYEKREISEMSGGQRQRVAIARAIVNEPKVLLLD
                     EPLSALDLKLRTDMQYELRDLQQRLGITFIFVTHDQEEALAMSDEIFVMNKGKIVQSG
                     TPVDIYDEPINHFVADFVGESNIVDGVMIEDNLVEFVGKQFECVDGGMRLNEPVEVVL
                     RPEDLTITTPDKGKLVVTVDTQLFRGVHYEIICYDEQQNEWMVHSTKKAKEGSKVGLA
                     FEPEDIHVMRFNESEEEFDARLDSYEE"
     gene            8576..9382
                     /locus_tag="IV334_002121"
     CDS             8576..9382
                     /locus_tag="IV334_002121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP8944178.1"
                     /translation="MKTMRRIYSVPYILWLGLFVIAPVLMIIYQSFFDMNGQFTLNNY
                     LSYFTSGTSLSMTINSVWYAFLITFFTLIISYPTAYFLTKLKHKQLWLMLIILPTWVN
                     LLLKAYAFIGIFSVHGSVNHFFDFIGIGPKQILFTDFSFLFVATYIEIPFMIMPIFNA
                     LEEMNPSLISASRDLGANSFETFRRVIFPLSLNGVKSGVQAVFIPSLSLFMLTRLIGG
                     NRVITLGTAIEQHFLVTQNWGMGSTIGVILIIAMFIVMLLTGEKKKGGRS"
     gene            9383..10210
                     /locus_tag="IV334_002122"
     CDS             9383..10210
                     /locus_tag="IV334_002122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP8944179.1"
                     /translation="MKKFKWSYLYLGIVFLFLYAPIFYLIFYSFNEGGTMNSFTGFTW
                     ENYEAVFEDTRLIIIVLNTFMLAFLSALIATIIGTFGAMGIYYTKKRRARTALLSMNN
                     ILLVSPDVIIGASFLIFFTLLGFGLGFQSVLLSHIAFSIPIVVLMVLPKLQEMNDSMV
                     DAARDLGANNFQVVKNIILPYLSPGIIAGYFMAFTYSLDDFAVTFFVTGNGFTTLSVE
                     IYSRARQGISLEINALSALVFLFSMILVIGYYFISKENRPKRLKKNRRVREGVARLR"
     gene            10207..11280
                     /locus_tag="IV334_002123"
     CDS             10207..11280
                     /locus_tag="IV334_002123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAP8944180.1"
                     /translation="MKKLQSLFLGILIIIAVLLLGVRQLEKTSGMSGAKVLTIYNWGD
                     YIDPSLITKFEKEYGYKVNYETFDSNEAMFTKIQQGGTNYDIAIPSEYMIQKMIKEKL
                     VLPLDHSKIKGLNNIDPRFLDLDFDPDNQYSIPYFWGTLGIVYNDKFFDANQIKHWDD
                     LWKPELKDSLMLIDGAREVMGLSLNSLGYSLNSKNMAQLTEAANKLNHLTPNVKAIVA
                     DEIKMYMINEEASVAVTFSGEAADMMWENEHLHYVIPPEGSNLWFDNIVMPKTVKNKE
                     GAYDFINFMLEPENAAQNAEYIGYSTPNEEAKALLPKDISSDEQFYPSDDTISHLEVY
                     KDLGSKYLGIYNDLFLEFKMYRR"
BASE COUNT         3529 a         2075 c         2417 g         3417 t
ORIGIN      
        1 actaagaagc gtgtgtttac caaagctata tgacttaaag aaaaacagag ttgatattgt
       61 gaaaaaaatc acttaatata gtataaggag aaaaggggga tacaaatgaa aaaattagtt
      121 gctggtttat taacagccgt tgcacttctt gctatgagcg catgcggcgc agatcagaaa
      181 aatacagcgc agacaagttc tagtacgaag gaatctgccg aagtatcatc aggcgcttct
      241 gaacaagtat atacagatcc aaaagaaatg aaagaatctt atgatgtcgt gattattgga
      301 gctggtggtg cagggatggc agcagctatc gaagcaaaag atgctgggtt gaatccagta
      361 attttggaaa agatgcctgt tgccggagga aacacatcta aatcatcaag cggtatgaat
      421 gcttcagaaa caaaatttca aaaagaagaa ggaatcaaag actcgaatga tgccttctat
      481 gaagaaacgc taaaaggtgg tggaggaacg aatgataaag aaatgcttcg atactttgtc
      541 gatcactctg ctgatgcggt cgattggcta gatcaaaacg gtatcaaact ggacaactta
      601 acgatcactg gagggatgag tgaaaagagg acacatcgtc cgtctgatgg ctcggctatt
      661 ggtggatact tagtggaggg gttattgaat aacgtccatg agcgggaaat ccctattttt
      721 gtcaacgcag atgtgacaga cattaagaaa aatggggaaa cggtcaatga agtgactgta
      781 catgttcaag gagaagaacc aaaagaggtc accggaaaag caatcgtagt taccacaggc
      841 ggttttggtg ccagcaaaga atttattgaa aaatatcgtc ccgatttagc tgattatatt
      901 acgacaaatc aagaaggatc aactggagat ggtatcacca tgattgaaaa attaggtgga
      961 caaaccgttg atatggataa aatacaaatt catccaactg tgcagcagga tgagggggtc
     1021 ttgattggtg aggttgttcg cggagaagga gcaatcttgg tcgatcaaga ggggaaaaga
     1081 tttgtcaatg aaatggatac aagggataaa gtatctgcag caattacagc attgcctgaa
     1141 aaatcagctt atttgatttt tgaccaaggc gtgcgtgatc gtgccacagc tattgaattt
     1201 tatgaccaaa aaggatatgt cacaactgga aagacaatgg aagaattagc agaaaaaatc
     1261 gatttaccaa aagaaacact agcaaaaaca gttgatggat ggaatacgga tgttgcggag
     1321 aaaaaagatg ctcaattcaa ccgtgccaca gccatggaac atgacttatc gtctccgaat
     1381 tattatgcga tcaaaatcgc accaggaatc cattacacga tgggcggtgt gaaaatcaat
     1441 acgaacactg aagtattaga taaagaaaac cagccaatta gggggttata tgctgctggt
     1501 gaagtcacag gtggcttaca tggaaacaac cggatcggtg gaaattcagt tgcggagatc
     1561 attatttttg ggcgacaagc gggcatacaa gcagctcgat ttgcttcgga aaatgaatga
     1621 aatagaacgt aaataaaaag aggttgtgaa atcagcgttt tgagctaata ccgagggtca
     1681 gctgattgaa cactgtttag tgaagaggtt gtgaaaaagc tgccctgcat caagtaataa
     1741 gaacagccaa gtaaaaacag cttcttatgt ttttaacttg gttggactta ttaccgcaga
     1801 tgcaagcttt tgaacaccgt ttattcggaa aaagagcgtg ggacaaaagt aaagaacact
     1861 tttgtctcac gctctaaaca cgtataaacg gcggaagcag aagaaattcc ttcggaaata
     1921 agctgaaatt cacaaaaatt tgaaaagcaa ttttcgtgaa tttcttctta tttcttggag
     1981 ttaaacgctt ctgtcccagc ctcttttttg atcagcatga ttttattcag ttatcttcaa
     2041 aatgattttt ccgatgtttt tgtttgcttc catatattca tgcgcttgtt tgactgcgct
     2101 gaattggaat gtacggtcaa tgattggccg aatcttgcca ttttctagaa aaggcccagc
     2161 tttttgcata aaagtttgtg cgagctctgc tttatactga tcacttctag gtgtcaataa
     2221 tgtaccaagc agttggacac gtttcccaat caatacacca aggtctatct caggcacgat
     2281 ggtcccacct agcataccaa tcaagaccca acgtccatca acggctatag aagctaaatt
     2341 cttttcccag taagaagcgc cgatgaaatc caaaataaca tcgactcccc gaccttcagt
     2401 gatttttcgt acctcttctt gaaagtcttg ttttttatag ttgatcaatt catctgcacc
     2461 aagttcttta caaaaggcga gtttttcttc tgatcctgca gtaacgatga ttttcgcgtg
     2521 agaaagctgt ctagctaatt ggatcgctgc tgttcccaca ccacttgctc ctgcatggat
     2581 caagacagtt tcgccttctt gtaattttcc aagccagtaa agtgtttgat aagcagtcaa
     2641 aaaaacttct gagattccag cagcagaaac atagtctagc gtatcaggga gaaggattgc
     2701 acgatcggca ggcatcacag catattcggc ataaccacct agattgacta atccgtatac
     2761 ttttgtccca ggagtaaaag tggagatatc acttttattc tcaactacct ctccagctac
     2821 ttcgactccc aaaatagggt agggaggttt caaagagact gattctcttt tcataatatc
     2881 tgttcgattg acagctgctg tatggacttt gatcagtaat tcgccttgtt ttggtttagg
     2941 aacaggcact tcaatttgtt ctaatacatc tacagaacca ggatgcttga ttgaaatggc
     3001 acgcatggat ctattcctcc tttgcccatt cttggaatgc tgtattcaac tctttgctac
     3061 cttcagaaag tactagagtc gtacgagaaa tatccgtttc ttccactaga gcggcaagta
     3121 cagcagcaac tgtagagcga gtgatcgttt ttgatgcctt ctcattaggc ttagttaatt
     3181 gaatggaagt aacttcttca tcgttagtca atgtgactgg ttgaagaatg acaaaatcta
     3241 agttagttcg tttcagccat tcatctgcat aatgtttagt aatataataa tctgtcatgg
     3301 actcaggcca ttttgctggt tcatctgcat aaacagcact gaccatcaga tagcgagaga
     3361 catgcgcatt ttcagctgcg atcactgttt tgattgcgcc atccaagtct acttgcagaa
     3421 gattctttcc ttgagagccg gcagcaaaaa tcaatacatc gatgcctttg aatgcttcgg
     3481 tcattttttc cacggaccaa gttaaatcaa aagagacata cgaaacattc tcttttttta
     3541 ctaatgtttg ggaagcaaca tctctcaccc ctgcaaaaat ttcatgggaa cttggagcta
     3601 aatcttttat caggtgtcgg ccaatttgtc cgtttgcacc tacaacaaaa attttcattt
     3661 ggatcatcct ttcgacgtca aatctttcca ttcaatagta acaccaaaag agaaaaaaat
     3721 cagagatcgg ctacgatgac tatcagttag taagagaagg gtgcttgaag tatcttaaca
     3781 agacattgca tactgctgca gtcgcaagac ctgcgacgat tccttcaggt ataccacttg
     3841 taaaaataac cgctagaata gctgaataaa cagcatttaa atttgttccg atgactttgg
     3901 cataatcttg ctggaataaa aagtagatca tattcatcac taaaatcgta ttggtcaatg
     3961 aaccggaaag tccagctaag aataaagaaa cggaatctgc tttttgtttc attagtttgc
     4021 gcagaccttt ataaacgaaa taaggaacca ccccaatcaa gatgcgagga ataaacgcga
     4081 tcagcaaggc tttccagctt ccatgttctg ttccgatgac tgggataaac ggagaaaaga
     4141 caaaagacaa aggtgtttgg ataactgtgc tacggatcat gctgatcagt ccaaatgtac
     4201 cgccaagaaa agcacctagt cttggtccta aaacgatcga agcaatgatt acaggaatat
     4261 gcatggttgt tgcattgatt ggaccgatag ggataaaacc taaaggcgta acagctaata
     4321 aaatcaaaat agctaaaaac atcgcagtta atgtaaagtt ttttgtattt ttcataagta
     4381 ctcctttttt ctaagcattt ctttcattaa gagtcagcat gacctgttcg atgatcgtct
     4441 gaatatcagc taaggcacct tttccaaaat ctccacaagc taaaagagat tctcttggtt
     4501 caatttcctg atagccgaca gatctcaatg tttccagatt acgctgagtg atcgggtttt
     4561 gatacatatt cgtgttcatg gcaggggcaa tcaattttgg tgttcccact ggaagcgcca
     4621 ttgcgactgt tgaaagaaga tcgtctgcaa tgccgtttgc tagcttgccg ataatgtttg
     4681 ctgtagcagg cgcgatgaga aacaagtcag cttgttttgc tagttcgata tgattgatta
     4741 cctgcggatt tgattcttgc atgacgtctg tatggactgc gcgcttcgac agagattgca
     4801 aggttaatgg cgtgataaaa tgtgtgctgc ttttagacat aatcacatct acctgggcac
     4861 ctaatttgac gaattgactt gtaagatcag cgcttttgta agcagaaata cttccagtaa
     4921 cgcctagtaa gatctttttc attttgattc ctccagtcta attttctcag taatcaatgc
     4981 agcaatctca gatttacttt gagcttcttc cactgttcca tctttagata agagatagcc
     5041 gtggtgtgtc tcgccatgga tttccgtcaa atcatttgcg aaaataaaat cagttttgtt
     5101 atttatcaga ctattttttg cgactgccag aagagattcc tttggtacat ctactaatag
     5161 tttgaaacca atgatttttg cttttggctg ccattcacgt agataagaaa tgatttttgg
     5221 tgttttcttc aaaacgagca caagatgctc ggtgttagaa gagatctttg aaacagtggt
     5281 atctgtctgt tcattttccg caaaccaatt gtccagttct tcttgcgttg aagaagtggg
     5341 taggtttttt gccagctgtt cttctgaaaa actaaaagca ggagtaaaat cactgactgc
     5401 catactgtgg ataatcgcat cgtagtgttc ttttttcgtt agttcttcta attgtaaaaa
     5461 taagtccagt gcagattcga tcgtataaat cgttatattg gagtgtcttt tcggtttcag
     5521 tgcttgtttc gtagtcacgt aatcgattgt ggtatccgga ttggcagcta aataatcagc
     5581 taatgcttgc cctaatcgtc ctgtcgaatg atttgtaata gcacgtactt gatcgatttt
     5641 ttcagtcgtt cctccagctg tgattagaat cttcatttat ggtcacctac atttactagt
     5701 gttcttcctt ggtgtgttcc agcaagtagt ttagatgctg tttcaggcaa ttgatccaga
     5761 gtaatctctt ggacgaccag ttcatctgca atctgcaaat ccgccatctg ttgccataaa
     5821 gaaagacgtt gatctattgg tacattgaca gaatcaattc caatcaactg gatgtttcgc
     5881 agaataaaag gcaagacagt cgcgtttagt ttgattcccc cagcattgcc acataaagct
     5941 actgcaccgt tataagagat caatggaaga agactgctta gctgctcgcc acctactgtg
     6001 tcgatgacat agtcgatttg ctgttttcct aaagcttttg ttgtctctgg cagaaattca
     6061 tcaggtcgta cgacttgcaa agcacctaga ctttttagcc atgtgacaga ctcttctttt
     6121 ctggaaaagg caatgattga ctggaatcct agtttatgta acagggctat cgctgtactg
     6181 cctacaccgc cagaagcacc agtaacgaca acttttttat cggaggtcat accttgacgg
     6241 attaaagcgt tcacacacaa catagcagta aaccctgctg taccaaggat catcgcttga
     6301 cgcagcgtca tattttttgg tagaggaact aaccagtctc ctggtacttt ttgatattgg
     6361 ctataaccgc ctggatgggt gacgccaagc ccaaaaccag tagcaagtac aagatcttct
     6421 tttgaaaaac gtggattgtt actttggacg acagttccgg ataaatcaat ccctggagtc
     6481 atcggatatg ttcgtatcac ctgaccagac tcgctcattg ccaatgcatc tttataattg
     6541 acatcagaat aggcaatttt taccaagact tcattttcag ccaactcagt aaatggcaga
     6601 tcactgattg ctgtatggaa aacaggttcc tttattacct gaaaagatcg aaaagcgttc
     6661 attcgtgaga cactcctttt aaaattttct tttatcatac cataaagcct actcttttat
     6721 tcttgttctt ccgtaaattt agacaaaaat taggtgaaaa ataaagaatc aaacaactag
     6781 aatttattcc aaaagataaa gaaatattca gcaaaaaaaa ttttacaaag ggttgccttt
     6841 ttcaataaat ttcgtatact aatacgagtg gtagtaaaca ggaagtttac tattagaaaa
     6901 cttcgattcg gaggaaatta tggaaattgg tgagaagtta agaaatttgc gcatccaaaa
     6961 gaatttgaca caagaagaac taggcgaacg cacagatttg tcaaaagggt atatttccca
     7021 gttggaacga gatttaagtt caccatcaat ggaaacattc ttttctattc ttgaagtctt
     7081 aggtgtgact ccagaagaat tttttcatca agaagcagcg aatctgcaag tggtttattc
     7141 aaaagaagat catacggttt atttcgatga agaaaacggg tatgaattgg aatggctcgt
     7201 ggctgattcg aatgaaaaag aaatggagcc tgtcttgctg acttttgaag gtgcaggaga
     7261 atataagacg tttgaacctt cattatctga aacgtttatt tttgtattag aaggggaatt
     7321 agagttaagc ttaggagaga cggtctatgt ggcaaaagca ggacagtcga tctattatca
     7381 agcgacaaaa caacatcaat taaggaatca tctcaaaaaa cgaacaaaag tattgattgt
     7441 tgcaacgcaa tcatatttgt aatatagggg gtaaaaacgt ggggaaacca atcatttcat
     7501 ttgatcaagt agtgaaacgc tatgatgatg aaacagtatt aaaaaaagta agttttgaga
     7561 tcgaacaagg aaaattttat acactgcttg gtccttccgg ctgtggtaaa acaacgatcc
     7621 tacgtattat agctggattc accgaagcta cagaagggga catctatttt gaagggaaac
     7681 ggttgaatga tttgccagct aataagcgac aagtaaatac agtcttccaa gattatgcac
     7741 tttttcctca tatgaatgtt tttgataacg ttgcttttgg attgaagatc aagaaaatgt
     7801 ccaaatcaga tatcgaaaag aaggtaaaag atgctttgcg tatggtccag cttcctggat
     7861 acgaaaaacg ggagattagt gaaatgtctg gtggtcaaag acagcgtgta gcgattgcac
     7921 gagcaattgt aaatgaaccg aaagtattgc tattagatga accgttatcg gcgcttgatt
     7981 tgaaactacg tacagatatg cagtatgaac tgcgggatct gcaacaacga ttaggaatca
     8041 cgtttatttt tgttactcat gaccaagaag aagcgttggc aatgagtgat gagatttttg
     8101 tcatgaataa agggaaaatc gtccaaagtg gtacacccgt agatatttat gatgagccaa
     8161 tcaatcattt cgttgctgat tttgttgggg agagcaatat cgtggacggt gtaatgatcg
     8221 aagataatct agttgagttt gttggtaagc aatttgaatg tgtcgatggc gggatgcgtc
     8281 taaatgaacc agttgaagtt gttttacgcc cagaggattt gacgatcacg acaccagata
     8341 aaggaaaact agtcgtaacg gtcgatactc agctgtttcg tggtgtccat tatgaaatca
     8401 tctgttatga tgaacagcaa aatgaatgga tggtccattc aacgaaaaaa gctaaagaag
     8461 gaagcaaagt aggattggcc tttgagccag aagatatcca tgtcatgcgt ttcaacgagt
     8521 ctgaagaaga attcgatgca cgtcttgaca gctacgaaga atagggggga caagaatgaa
     8581 aacaatgcgc aggatctatt ccgtccctta tattttgtgg ctcggtttat tcgtgatcgc
     8641 gcctgtatta atgatcattt atcaatcttt ttttgatatg aatgggcaat tcacgctgaa
     8701 taattatcta tcttatttta cgtcggggac atcgctttct atgacgatca attctgtatg
     8761 gtatgcattt ttgatcacgt ttttcacgct gatcatcagt tacccgactg cttatttttt
     8821 aacaaaactg aagcacaagc aattatggct gatgctgatt attttgccta catgggtgaa
     8881 tctgctgctt aaagcttacg catttattgg gattttcagt gtgcacggca gcgtgaatca
     8941 tttctttgat tttatcggga tcgggccgaa acagatatta ttcacagact tcagttttct
     9001 gttcgtggca acgtatatcg agattccatt tatgattatg ccaattttca atgcattgga
     9061 agagatgaat ccttctttga tcagtgctag tagagattta ggcgcgaata gctttgagac
     9121 cttccgacga gtgatttttc cgttatcatt aaatggtgtg aaaagtggtg tccaagcagt
     9181 atttattcct tctctttctt tattcatgct gactcgtctg attggcggaa atcgggtgat
     9241 cactttagga acggctatcg aacagcattt cttggttaca caaaactggg ggatgggttc
     9301 tacaatcggt gtgattttaa tcattgctat gttcatcgtg atgctcctga caggcgagaa
     9361 gaaaaaagga ggtcgctcat aaatgaaaaa atttaaatgg tcgtatcttt atttagggat
     9421 cgtcttttta ttcttatatg caccgatctt ctatctgatt ttttattcct tcaatgaagg
     9481 aggaacgatg aacagcttta caggatttac ttgggaaaat tatgaagctg tttttgaaga
     9541 tacacgtttg atcattatcg tattgaatac gttcatgctg gctttcttat ctgcattgat
     9601 cgccacgatc atcgggacat ttggggctat gggaatctat tacacgaaaa aacgacgtgc
     9661 acggacagcg ttgttgagca tgaataacat cttattagtc tctcctgatg ttattatcgg
     9721 tgctagtttc ttgattttct ttacactact aggattcgga ttaggttttc agagtgtatt
     9781 gctttcccat attgctttca gcattccaat cgtcgtcttg atggtcttgc caaaattgca
     9841 ggaaatgaac gattcaatgg tcgatgcggc tagagacctt ggtgctaata atttccaagt
     9901 agtcaaaaat atcattttgc cttatctttc tccaggaatc atagctggat attttatggc
     9961 atttacctat tctcttgatg attttgcggt tacatttttc gtcacaggaa atggctttac
    10021 gacgctctca gtagagatct attctcgtgc aagacaagga atcagtttag aaatcaatgc
    10081 gctaagtgcg ttagttttct tgttctccat gatattggtc attggatact actttattag
    10141 taaagaaaac cgtccaaaac ggctgaagaa aaacagaaga gtacgcgagg gggtggcacg
    10201 gctgcgatga aaaaattaca atctctgttt ttaggtattc tcatcattat tgcggtgctg
    10261 ctgcttggtg ttcgtcaact tgaaaaaaca agtggcatga gtggagcaaa ggtacttacc
    10321 atctataact ggggagatta tatcgatcct agtttgatca cgaaatttga aaaagaatat
    10381 ggatacaaag tgaattatga aacatttgat tctaatgaag cgatgttcac aaaaatccag
    10441 caaggtggga caaactacga catcgctata ccaagtgaat atatgatcca aaaaatgatc
    10501 aaagaaaaac tagtacttcc attagatcat tcgaagatca aaggattaaa taatattgat
    10561 ccacgatttt tagacttgga ttttgatcca gataatcagt attcgattcc atatttttgg
    10621 ggaactttag ggatcgtata taacgataaa ttctttgatg caaatcaaat taagcattgg
    10681 gatgatttgt ggaagccgga attaaaagac agtttgatgc tgattgacgg tgcacgagaa
    10741 gttatggggc tttctttgaa tagtttaggc tattctttga atagtaagaa tatggcacag
    10801 ttgacagaag cagcaaataa attgaatcat ttaacaccaa atgtcaaagc aatcgtggca
    10861 gatgagatca aaatgtatat gatcaatgaa gaagcctccg tggcagtgac attttctggc
    10921 gaagctgccg atatgatgtg ggaaaatgaa catttacatt atgtgatccc accagaagga
    10981 tccaatcttt ggtttgataa tatcgttatg ccaaaaaccg tgaaaaataa agaaggtgcc
    11041 tatgatttca tcaactttat gctagaaccg gaaaatgcgg cacaaaatgc agagtatatt
    11101 ggttattcta caccaaatga agaagcaaaa gcgttattgc caaaagacat ttcgtcggac
    11161 gaacaattct atccatctga tgacacgatc tcccatcttg aagtctacaa agatctagga
    11221 tcgaaatacc taggcatcta taatgaccta ttcttagaat tcaagatgta taggagataa
    11281 tggcaaaact cagtaagaat ggggacttat caacgttcta aagagctaaa aatcgtcctt
    11341 tttctatctg agttttccaa tcttctacca aaagcggatg aaacgccgct ttttttgtgt
    11401 tattttattt tttctaccac gtttttctac cagagaaa
//
LOCUS       DACDKM010000087        11390 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20834_2016
            SAMN12640370-rid9891663.denovo.087, whole genome shotgun sequence.
ACCESSION   DACDKM010000087 DACDKM010000000
VERSION     DACDKM010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640370
            Sequence Read Archive: SRR10040891
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11390)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11390)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742835.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742835.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:04:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,012
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 29 of 75
            Pseudo Genes (incomplete)         :: 33 of 75
            Pseudo Genes (internal stop)      :: 19 of 75
            Pseudo Genes (multiple problems)  :: 5 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11390
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640370-rid9891663.denovo.087"
                     /isolate="E20834_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(472..1158)
                     /locus_tag="IV334_002124"
                     /pseudo
     CDS             complement(472..1158)
                     /locus_tag="IV334_002124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374310.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
     gene            complement(1294..1488)
                     /locus_tag="IV334_002125"
     CDS             complement(1294..1488)
                     /locus_tag="IV334_002125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8944181.1"
                     /translation="MKRKKQVIPMYVVKVMHGYIDKTGCRTREKNLDNLLIFKDKKES
                     EAFAKRIGGRVKPIQEVRPD"
     gene            complement(1538..1978)
                     /locus_tag="IV334_002126"
     CDS             complement(1538..1978)
                     /locus_tag="IV334_002126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HAP8944182.1"
                     /translation="MEKFTGYVREPFYYETDQMGIIHHSNYIRWFEEARVALLVHLGF
                     PYKKIEESGIIIPVLEVNCSYKEMIHYGEKVSIHPTIQKYTGTRLDFSYEIVGTEDKK
                     LKTTGSSKHCFLLSDSQRLVKLSKVNPELDQLFQAYASLDDVNS"
     gene            complement(1982..2827)
                     /locus_tag="IV334_002127"
     CDS             complement(1982..2827)
                     /locus_tag="IV334_002127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HAP8944183.1"
                     /translation="MLINEQLVKRVITPRPKISHKGTFGRIVLIGGNEQYGGAIIMSA
                     LAAVHSGAGLTTVVTDEKNRSALHSHLPEAMIVVWTDTDQFLSVIKQADVLLIGPGLG
                     TSSKSLERFKQVLENQSKEQWLVIDGSALSLLAANHLLLPYPEQTVLTPHQMEWQRLS
                     SLPIQEQRDKENKKKQQEIGAVLVLKSHETKIYGHQHFYTNPLGSAAMATGGMGDTLA
                     GMITGFLAQFPQNDQTIAAAIYLHSFIGDQLAKEQYIVLPTQISEALPKWMNYFCKKS
                     MENEA"
     gene            2976..4394
                     /gene="pepV"
                     /locus_tag="IV334_002128"
     CDS             2976..4394
                     /gene="pepV"
                     /locus_tag="IV334_002128"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase PepV"
                     /protein_id="HAP8944184.1"
                     /translation="MTIDWQKEVEARKEDLLEDLKNLLRVNSERDDSKVTPDAPFGPG
                     PRDALKHMLAYGERDGFKVKNVDNYAGHIDLGEGDETLGIFGHMDVVPAGDGWDTDPY
                     EPVIKDGKIFARGSSDDKGPSMAAYYAMKIIKELDLKLSKKVRFVVGSDEESGWGDMA
                     YYFEHEEEPDFGFSPDAEFPIINGEKGNVSLALRFKGDNAGDYVLKSFVSGLRENMVP
                     GTATAALQVPSADAAIAMEEAFYQFIEANPVSGTIEADNTYVKIELVGKGAHGASPQS
                     GINAGSFLALFLDNYEFLGSAKQFIHVAAAYVHEDFYGEKLGVAYEDEKMGKLTMNAG
                     LFAFEENGTEEANFINMNFRFPKGVTVDGLQSDIEQTVGQEGATVTRGARVMEPHYVP
                     MDDPLVATLLQVYEDHTGEKGYEQIIGGGTYGRLLKRGVAYGAMFPGYTDTMHQANEF
                     MSLDDLFRATAIYADAIYRLAK"
     gene            complement(4451..5398)
                     /locus_tag="IV334_002129"
     CDS             complement(4451..5398)
                     /locus_tag="IV334_002129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HAP8944185.1"
                     /translation="MEQQEKIAVLIPCYNEEATISTVIADFKRELPEADIYVYDNNST
                     DRTYELAVQGGAIVKKEPRQGKGNVIRQMFFDIDADYYLMVDGDDTYPAEAVHGLLEK
                     LRSGEADMVIGDRLSNGTYFDENKRPFHDFGNNLVRNTITRMYKTKILDVMTGYRGFN
                     RIFVKSFPIMSSGFQIETELTIHALDKKFKFVEIPIDYRDRPEGSESKLNTFSDGFKV
                     IMMIIKMFKDYKPLLFFGIWTIFFFLFGLVTGIPVIREYMLTSFITRIPSAILSTGLM
                     TLALLSLVTGLILDTVVTNAKKEYELNLYHVYHEYHSRK"
     gene            complement(5621..5971)
                     /locus_tag="IV334_002130"
     CDS             complement(5621..5971)
                     /locus_tag="IV334_002130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HAP8944186.1"
                     /translation="MYEESELSYFKGGLAVGIGLGLLSGVASTIWYHRKKVLSADQVL
                     ENIKSSFLKEGAIEGSWISFEKEPIRKFAVRSSAYRGGLSRIEDGETVYYEFLADAHT
                     GTVLEISRKKGATD"
     gene            6171..7250
                     /gene="pepA"
                     /locus_tag="IV334_002131"
     CDS             6171..7250
                     /gene="pepA"
                     /locus_tag="IV334_002131"
                     /EC_number="3.4.11.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl aminopeptidase"
                     /protein_id="HAP8944187.1"
                     /translation="MEEKTFQRIKELTELQGTSGFEQDVRAYMESHMEPLVDELQLDG
                     LGGIFGLRHHEEADAPRVMVAAHMDEVGFMLTQIQENGLFKVVPLGGWNPYVVSAQRF
                     TLKTSTGKNYPCISSSVPPHLLRGTSGQKSVEVTDILFDAGFESREEAMSYGVLPGDT
                     IVPYAETIKTANGKNIISKSWDNRYGCTMVLEALEALQNETLGHTLIAGANVQEEVGL
                     RGSKASVNKFKPDLFFAVDCSAADDTVTKNGTFGHLGEGTLMRIQDPGLIMLPRLREY
                     LLDIAETNNIPYQYFVSKGGTDAGAAHTQNEGIPSTVIGVVGRYIHTHQTMFSIRDFE
                     AAREMLIQTLKGLDKSTVNTIVYGK"
     gene            7394..7714
                     /locus_tag="IV334_002132"
     CDS             7394..7714
                     /locus_tag="IV334_002132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HAP8944188.1"
                     /translation="MIIPNSLEELAGYVETGKSVFFFTAGWCGDCRFIKPQMPEIEND
                     FSDWQFIEVDRDKYIDVAAEWNIFGIPSFVVIQDGKELGRLVNKDRKTKQEIESFLES
                     VTKG"
     gene            7736..8200
                     /locus_tag="IV334_002133"
     CDS             7736..8200
                     /locus_tag="IV334_002133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HAP8944189.1"
                     /translation="MNQEYKNILVGIDGSEQALAAFKKAVEVARRNNGKVYVANVIDQ
                     QFYNFMGYAPIDQNLVDQQTETAKEMIEECKEYGKSVDYTNIEGIIAYGSVRESMAVK
                     LPKKYEIDLIMVGQSGLNAVERFMTGSVASYVIRHSPCDVLIVSDEEEQPSN"
     gene            8405..9010
                     /locus_tag="IV334_002134"
     CDS             8405..9010
                     /locus_tag="IV334_002134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4479 domain-containing protein"
                     /protein_id="HAP8944190.1"
                     /translation="MIFAYNKAHVGDTLMVIIADDKGTENTVERKWNVARIKDEQGQV
                     VAWNFFHISDHLTIEGNGQVSLTDEELAKLNRLIKEAGFEEQLEADHEPKIVVGYVKT
                     CVPHPDSDHLSITETEVDNGTVLQIVCGAPNIEAGQKVVVAKPGAMMPDGMMIWPGTL
                     RGVDSYGMICSARELHLPNAPAKKGILVLPEDAVVGEAFPK"
     gene            complement(9049..10338)
                     /locus_tag="IV334_002135"
     CDS             complement(9049..10338)
                     /locus_tag="IV334_002135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HAP8944191.1"
                     /translation="MDRKNVTPKMKKSKNNSLWRKLGLGLVGGIVGGLLTAGIFYAAM
                     GTGNNSSTATSGNQNSAGETVVENVKVNVDSDITKAVDKVQDAVVSVINLQSQNQSSG
                     FGGLFGQQEESSSSDDSNLEAYSEGSGVIYKKDGNTAYVVTNNHVVDGQQGLEVLMKD
                     GTKVKAELVGTDAYSDLAVLKINSDKVETVASFGDSSALKVGEPAIAIGSPLGSEYAN
                     SVTSGIISSLNRQVTSTNESNETVNINAIQTDAAINPGNSGGPLVNIEGQVIGINSSK
                     IASTSESTSNVSVEGMGFAIPSNDVVNIINQLEKDGKVTRPALGITMVDLSAVSTQQQ
                     EQILKIPSSVTNGVIVTNVQTATPAEKAGLKQYDVITKIDDKEVSSGVELQSALYKKK
                     VGDSVKITYYEGSKEKTTTVELTIDQSALKQNQNSEN"
     gene            10766..11245
                     /gene="rlmH"
                     /locus_tag="IV334_002136"
     CDS             10766..11245
                     /gene="rlmH"
                     /locus_tag="IV334_002136"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HAP8944192.1"
                     /translation="MNIKIISVGKLKEKYLIQGINEYVKRLNAYAKIELIEVPDEKAP
                     ENLSEAQMRQVKEKEGERILAKIKEQEYVYALAIEGKNPTSESFAKQIDQLGIQGKSH
                     LVFVIGGSLGLSEAVMKRSNAQISFGKMTYPHQLMRLILVEQIYRAFRINAGAPYHK"
BASE COUNT         3587 a         2150 c         2240 g         3413 t
ORIGIN      
        1 gatcttagaa gaaataaaaa aaatagaaaa aagatgtacg gagtatgatt tagattacaa
       61 ataaagagac tgtgacaaaa gtcgtgtcac agtccttttt ttccgaataa acggtgttct
      121 cctgtcaact cctcggtcac aagtcaaaat attcaacaaa catgagaagc tgttttttga
      181 aattgttcct tgtgactcgg agatgaacgg caggctcacg acctcttgga aaacggtgtt
      241 caaaagcttg catctgcggt aataagtcca gccaagttaa aaacataaga agctattttt
      301 acttggctgt tcttattact tgatgcaggg cagctttttc acaacctctc aataaacggt
      361 gtgcaatcag ctgaccctcg gtattagctc aaaaaaaacg gcaaaatatg aggaactatt
      421 ttgccgtttc tttctctaat actcaggtca aaacgctgat tccaaaacct cttatttgat
      481 gggtctgtcc tgaatatctt caaaataatc ggggtaatta tgaatcacgt attcttgctc
      541 ctcgataatc atatgtaaat gcatcgtgca aagaaatccg acttcatcaa agttatgatc
      601 aatcattgat tgaagcattg tctgatgttc atctaatact cgaccccagt ctaattctga
      661 aatcgtcaaa cgtaaccatc tgaaacggtt caagtgcgta ctataacttt ctagccaatc
      721 ccaaatttct cgttttccag caatttcata acaagttcgg tgaaaatcag tatccaaatc
      781 aaaaaaagct tttttatttt gagcgattac tgcctgatcg gtttgttcta gtattttttc
      841 tagtttagct tgatcttact tggtcatttt cgcactgcat tcttgcagga tcggtttttc
      901 caacttttcc cgcgtataac gggctaatga agcagatcgg atatcgattt ttgaaacata
      961 agtgccggac tgtggaatgg tttttacaag ctcttgattg cgcagttgga tcagtgcttc
     1021 tcgaatcggt gtacgcccga tagctagttg ttcttctaac actttatcgg agattttact
     1081 tccaggtgct aactcggaat aaatgatttg tttacgtata gattcataag cttgctgttg
     1141 caaattattg ataaccattt aaccttcctt tctatgcaga tataatctat tataacataa
     1201 atcgcttaca aatcgtgtgg gcataaaaac acagtctgca aaaaaagccc tgataggcat
     1261 gcctattaga gcctcgaaaa gaaaaatatt gaatcaatct ggtcgtacct cttggatcgg
     1321 tttgacacga ccgccgattc ttttagcaaa agcttcggat tcttttttgt ctttgaaaat
     1381 gagcagattg tctaagtttt tttcccgagt gcgacagcca gttttatcga tgtaaccgtg
     1441 cattacttta acgacataca tggggatcac ctgctttttt cttttcatta tacgaactac
     1501 tcgacacaat acaagaaaaa tgcttagacg gtctttatta agaatttaca tcatccaaag
     1561 aagcgtacgc ttggaataac tgatctaact ccggattgac tttgctcagc ttcactaatc
     1621 gttgcgaatc agaaagtaaa aaacagtgtt tactactgcc agtagttttt aattttttgt
     1681 cttcagttcc cacaatttcg tagctaaaat ccaaccgtgt ccccgtatac ttttggatag
     1741 ttggatggat gctcactttt tctccgtaat ggatcatttc tttatagctg caatttactt
     1801 ccagaacggg gatgatgatc ccagattctt cgattttttt atagggaaac cctagatgta
     1861 ccaataaggc tacccgagcc tcttcaaacc agcggatata attgctgtga tgaataatcc
     1921 ccatttgatc tgtttcataa taaaaaggtt ctcgcacata acctgtaaat ttctccatga
     1981 ctcatgcctc gttttccatt gattttttac aaaaataatt catccatttc ggaagtgctt
     2041 cgctgatttg tgtcggcaaa acaatatatt gttcttttgc cagctggtct ccaatgaaac
     2101 tatgaagata aatagctgct gcaatcgttt ggtcattttg aggaaactgg gctaaaaatc
     2161 ctgtgatcat cccagctaaa gtgtcaccca tgccgcccgt tgccatagca gcagaaccta
     2221 aaggattggt gtagaagtgc tggtgaccat aaatttttgt ctcatgactt ttcaaaacga
     2281 gcacagcacc gatttcctgt tgcttctttt tgttttcttt atcccgttgt tcttgtatcg
     2341 gtagagaact caaccgttgc cactccattt gatgaggtgt aagtacagtt tgttcgggat
     2401 agggtagcaa aagatgatta gcagcaagta aacttaaagc agagccatca atcaccagcc
     2461 attgttcttt tgattggttt tctagtactt gtttgaaacg ttccaaactc ttagaagaag
     2521 tacccaatcc gggaccaatc aagagtacat ctgcttgctt tattacggaa agaaactggt
     2581 cagtatccgt ccaaactaca atcattgctt ctggcagatg actgtgaaga gcactccgat
     2641 ttttttcatc agttactaca gtcgtgagac ccgctccgct gtgtacggca gctaaagcac
     2701 tcataatgat tgctccgcca tattgttcat tgccgccaat caaaacgata cgtccaaatg
     2761 ttcctttatg tgaaattttt ggacgaggtg tgatgactct tttcactagt tgttcgttta
     2821 tcagcatcta aaaacctcct tttactcatt atagcgtata ttttttttag agaaaaacag
     2881 ataagaaaag gaattgtcag attttttgaa ctattttttt ctgtaatttg tggtatcatg
     2941 aacacgaaga cttaaataag tggaggatta actttatgac aattgattgg caaaaagaag
     3001 tagaagcacg taaagaagac ttgcttgaag acttgaaaaa tcttttacgt gtcaacagcg
     3061 agcgcgatga ctcaaaagta acaccagatg caccatttgg acctggacct cgcgacgcat
     3121 tgaaacatat gttagcttat ggcgaacgtg atggatttaa agtaaaaaat gtcgataact
     3181 atgcagggca tattgatctt ggcgaaggag acgaaacgct agggatattt ggacacatgg
     3241 atgtcgttcc tgcaggagat ggctgggaca cagatcctta tgaaccagta atcaaggatg
     3301 gaaagatctt tgcacgcgga tcaagcgatg acaaaggtcc aagtatggca gcttactatg
     3361 cgatgaagat cattaaagaa cttgacttga aactatcaaa aaaagttcgc ttcgtagtcg
     3421 gcagcgatga agaaagcggc tggggcgata tggcttatta tttcgaacat gaagaagaac
     3481 cagattttgg tttctcacca gatgctgaat tcccaatcat caatggtgaa aaaggaaatg
     3541 tttctttagc tttacgcttc aaaggcgaca atgcaggaga ttatgtcttg aaatcttttg
     3601 tttccggact tcgcgaaaat atggtgccag gaacagcaac tgcagcactt caagtaccaa
     3661 gtgcagatgc agctatcgcg atggaagaag cattttacca attcatcgaa gcaaatccag
     3721 taagcggaac gattgaagcg gacaatacgt atgtaaaaat cgaattagtt ggtaaaggcg
     3781 cacatggtgc aagcccacaa tctggtatca atgcaggttc tttcttggca ttgttcttag
     3841 ataactatga attcctagga tctgctaaac aatttatcca tgtagcagct gcttatgtcc
     3901 atgaagattt ttatggagaa aaactaggag tcgcttatga agatgaaaaa atgggcaaat
     3961 tgacaatgaa tgctggacta tttgcttttg aagaaaatgg tacagaggaa gcgaacttca
     4021 tcaatatgaa tttccgtttt ccaaaaggtg taacagtaga cggattgcaa agtgacatcg
     4081 aacaaacagt ggggcaagaa ggcgcaacag ttactcgagg tgctcgcgta atggaaccac
     4141 attacgtacc aatggatgat ccattggtgg ctacattatt gcaagtctat gaagaccaca
     4201 ctggagaaaa aggctacgaa caaatcatcg gtggcggaac ttacggacgt ttgttgaaac
     4261 gtggcgttgc ttatggagcg atgttcccag gatatactga tacaatgcat caagcaaacg
     4321 aatttatgag tcttgatgac ttgttcagag caacagcaat ctatgcagat gccatctatc
     4381 gtttggcgaa ataattaatt aaaaagagga tcaagaattg caaattattt ttgcagtctt
     4441 gatccttttt ttactttcta ctgtggtatt catgataaac atgatataga ttcaattcat
     4501 attctttctt agcgttcgtg acgactgtat ccaaaatcaa ccctgttact aatgaaagaa
     4561 gtgctaaagt catcaatcca gtacttaaaa tcgcagaagg gatgcgtgta ataaagcttg
     4621 tcagcatata ttcgcgaatc acaggaatcc ccgtgaccaa tccaaataaa aagaagaaga
     4681 tggtccaaat cccaaagaaa agcaaaggct tataatcttt aaacatttta ataatcatca
     4741 tgatcacttt gaatccgtca gagaatgtat tcaattttga ttcgctgcct tcaggacgat
     4801 cacgataatc gattgggatt tcaacgaact tgaatttttt atctaaagca tggatcgtca
     4861 attccgtttc gatttgaaaa ccagaactca taataggaaa acttttgaca aagatacgat
     4921 tgaatcctcg atagccggtc atgacatcaa gaatttttgt tttatacatt ctagtaattg
     4981 tatttctaac aaggttattt ccaaaatcgt ggaaaggtct cttattctca tcaaagtagg
     5041 ttccatttga taggcgatca ccaatgacca tatctgcttc accagaacgt aatttctcga
     5101 gcaaaccatg tacagcttct gcaggataag tatcatcgcc atctaccatt aaataatagt
     5161 cggcatcgat atcaaagaac atttggcgga tcacatttcc ttttccttga cgaggttctt
     5221 tttttacgat tgctccacct tgaacggcca attcgtatgt tcgatcggtc gaattattat
     5281 catagacata aatatctgct tctggtaatt ctcgtttaaa gtctgcaatg actgttgaaa
     5341 ttgttgcttc ctcattgtaa caaggaatca gaacagcaat tttttcttgt tgttccattt
     5401 ttcactctcc ttaactatag aaaacaaaag ctaaattaaa aatacctcat aatcaaagga
     5461 aatgctagtt tttcccgcta aatatcattt atttaaaaag aaaatactgg aatatatgtt
     5521 tggaaagctc tatctaaaaa accacccact acaaaagaag gacatgatac ctctcttttg
     5581 aatagtgggt gcgtattcgt tctctcactt ttttgacgta ttaatctgtt gcaccttttt
     5641 ttcttgaaat ttctaatacg gttcctgtat gagcatctgc taaaaattca taataaacag
     5701 tttcgccatc ttctattcga gaaagacctc cccgataagc actcgaccga acggcgaatt
     5761 tcctaatagg ttccttttca aaagagatcc aagaaccttc gattgcacct tcttttagaa
     5821 aagatgactt gatattttca agtacttggt ctgctgacaa tacttttttg cgatgatacc
     5881 aaatcgttga agctaccccg ctcaataatc ctagaccgat tccaactgct aacccgcctt
     5941 taaaatagct tagttcgctt tcttcataca tgtggacata cctccttctt ctcacttgtt
     6001 actccttgtg cttattttac cacactctca tattttcttt gaaataaata accgctacca
     6061 gaaaaaataa gctataataa atacgatcgg tctgcagaaa aaatcatatt ttatccgcag
     6121 atcaatataa gtctgagagg agtttagtct gaaatttaga cgaatagcat atggaagaaa
     6181 aaacatttca acgaatcaaa gagttaacag aattacaagg aacaagcggt tttgaacaag
     6241 atgttcgcgc ttatatggaa agtcatatgg aaccgctggt agatgagtta cagttagatg
     6301 gtttgggcgg catttttggt ctgcgccatc atgaagaagc ggatgcaccc cgtgtaatgg
     6361 tcgcagcaca tatggatgaa gtcggattca tgctgacaca aatccaagaa aacgggcttt
     6421 ttaaagtcgt acctctaggc ggctggaatc cttacgttgt ttctgctcag cgttttacac
     6481 ttaaaacctc tactggtaag aattatccat gtatttcttc ttccgttccc ccacatttat
     6541 tgcgaggaac aagcggacaa aaatcagtag aagtaaccga tattttgttc gatgctgggt
     6601 ttgaatcaag agaagaggca atgagttacg gtgtccttcc aggagacaca atcgttcctt
     6661 acgctgaaac gatcaaaaca gccaacggaa agaatattat cagtaaatca tgggacaacc
     6721 gctatggctg tacaatggta ttagaagctt tagaagcgtt gcaaaatgaa acattaggtc
     6781 atacattgat tgcaggcgcg aatgtccagg aagaagtagg cttgcgtggt tctaaagctt
     6841 ctgtcaataa attcaagcca gatctgttct ttgcagtaga ctgctctgca gcagatgata
     6901 cagtgacgaa aaatggcaca ttcggacatt taggagaagg tacattgatg cgtatccaag
     6961 atccaggttt gatcatgctt cctcgtttgc gcgagtactt gctggatatc gctgaaacaa
     7021 acaatatccc ttatcaatac tttgtttcaa aaggcggtac agatgcaggt gctgctcata
     7081 cacaaaacga aggaatccca agtacagtga ttggcgtagt gggacgttat atccatacgc
     7141 atcaaacgat gttcagtatt cgcgattttg aagctgcacg agaaatgttg attcaaacac
     7201 tgaaaggttt ggataaatca acagtaaata cgatcgtata cggaaaataa tgcgatagtg
     7261 aaactataaa atctggcaaa agattgcttt ttgccagatt ttttttataa aaagaggatc
     7321 aatgtttgtt agttcgaaag aaaggtgtat aatttaattg aaaaatacat aaacagaaag
     7381 tagaggacaa agaatgatta tcccaaatag tttagaagaa ttagcaggct acgtagaaac
     7441 tggaaagagt gtatttttct ttactgccgg ctggtgcgga gactgccgtt ttatcaaacc
     7501 gcaaatgcct gaaattgaaa atgacttttc tgattggcaa tttattgaag tagaccgtga
     7561 caagtacata gatgtagccg ctgaatggaa catttttggt atcccaagtt ttgtcgtgat
     7621 ccaagacgga aaagaattag gtcgtctagt gaataaagat cgtaaaacaa aacaggaaat
     7681 cgaatcgttc ctagaaagtg taacaaaagg ctgacatctg ttaggagggg tcaagatgaa
     7741 tcaagaatat aagaatattt tagtaggtat cgacggtagt gaacaagcac ttgctgcttt
     7801 caaaaaagcg gtagaagtgg ctcgccgtaa taatgggaaa gtatatgtag caaacgtaat
     7861 cgatcagcaa ttttataact ttatgggcta tgcacctatt gatcaaaatc tagtcgatca
     7921 gcaaactgaa acagccaagg aaatgatcga agaatgtaaa gaatatggga aatctgttga
     7981 ttacacaaat atagaaggaa ttattgcata tggttctgtt cgtgaatcca tggcagtcaa
     8041 attgccaaaa aaatatgaga tcgatctaat catggttggg cagtcaggtc taaatgcagt
     8101 cgaacgcttt atgacaggaa gtgtagccag ctacgtgata cgtcattcac cttgtgatgt
     8161 cttgatcgtt tctgacgaag aagaacagcc aagtaattaa gcaagtggaa cttgtacgac
     8221 ctttttaatc aattaactaa aacagtaaag ggactgtgac aaaagtcttg tcacagtccc
     8281 ttgttacgaa taaatggtgt tccagaagag gcttcttcgg aaataaagca tgaaaaaaat
     8341 ttcatgcagt gaatgcaatt ttttgttaag ataaatctca gcaaaggata atggaggaaa
     8401 gaacatgatt tttgcttata acaaagcaca cgtcggagac accttgatgg ttattatagc
     8461 cgacgataaa ggaacagaaa acactgttga acgcaaatgg aatgttgctc ggatcaaaga
     8521 tgaacaaggt caagttgttg cttggaactt tttccatatc tccgatcatc taacaatcga
     8581 agggaacggc caagtaagct taacagatga ggaattagcg aaattgaatc gattgatcaa
     8641 agaagcagga ttcgaagaac agcttgaagc tgatcatgaa ccgaagatcg ttgtcgggta
     8701 cgtaaaaaca tgcgtcccac atcctgattc tgaccattta tcgattactg agacagaagt
     8761 agacaatggt acagtattgc aaatcgtttg cggtgcaccc aatatcgaag ctggacaaaa
     8821 agttgtcgtt gcaaaaccag gagctatgat gccagatgga atgatgatct ggccaggaac
     8881 tttgcgaggg gtagatagct atggaatgat ctgttccgct cgtgaattgc atttgccaaa
     8941 tgctccagca aaaaaaggaa tcttagtatt gcctgaggat gccgtagtcg gagaagcatt
     9001 tccaaaataa agaggttgtg acaagtcttt gtcacaacct cttttttatt aattttcact
     9061 attttgattt tgttttaatg ccgattgatc gatcgtcaat tcaacagtag ttgttttttc
     9121 tttcgagcct tcatagtaag tgattttgac agaatctccc acttttttct tatacaaagc
     9181 agattgaagt tctacaccag aagaaacttc tttgtcatcg atcttagtaa tgacgtcgta
     9241 ttgtttcaat ccagcttttt cagccggtgt agctgtttgg acatttgtca cgatgacacc
     9301 gttagttaca gatgaaggaa tcttcaagat ttgttcttgt tgctgagtag aaacggcaga
     9361 taagtcgacc attgtgatac ctagagctgg acgtgtcact tttccgtctt tttccagttg
     9421 attgatgata ttcactacat cgttacttgg aatagcaaat cccattcctt ctacgcttac
     9481 attagaagta gattcagaag tacttgcgat cttacttgag ttgataccga taacttgtcc
     9541 ttcgatatta actaaaggac caccagaatt tccagggttg attgctgcat ctgtttggat
     9601 cgcattgata ttgactgttt cgttcgattc attcgtactt gtcacttgtc ggttcaagga
     9661 agaaatgatt cctgaagtga cagaatttgc atattcagat cctagtgggg aaccaatcgc
     9721 aatcgcaggc tcaccaacct tcagagcact tgagtcacca aaagaggcaa ctgtttcgac
     9781 tttgtctgaa ttgatcttca acaccgctaa atctgaataa gcatctgttc caactaattc
     9841 agcttttacc ttcgtaccgt ctttcatcaa gacttcaaga ccttgctggc catctactac
     9901 gtgattattc gtgactacat aagcagtatt cccgtctttt ttgtagatca cgccgcttcc
     9961 ttcactataa gcttccaaat tactatcatc tgagctgcta ctttcttctt gctgcccgaa
    10021 tagtccgcca aaaccgcttg attggttttg actttgtaag ttgataacag aaacgacagc
    10081 gtcttgaact ttatctaccg ctttggtgat atcagaatct acattgactt tgacattttc
    10141 cacaactgtt tcaccggcag aattctgatt gccgcttgta gcagtagaac tattgttgcc
    10201 agtgcccatc gctgcataaa aaatgccagc tgtcagtagt cctccaacga tccctccaac
    10261 aagccccaga cctaacttac gccagaggct attattttta ctttttttca tctttggtgt
    10321 cacatttttt ctatccatgt gttcctcctc caattacatt catttatcta ctgttagtat
    10381 agtcttattt gttgacttta gtctactttg agactgtgaa ctttttatga aaaactttag
    10441 gaaaaatcat tgttttcaga tgaaaatatt tgattttctt tttccacaga tactgtgtaa
    10501 aactacgcgg ataggattgg aaaaataagg gataaaacat atccacagcc tgtgtataaa
    10561 gtggataaat tcttataaaa cgtatgtttg cctaatgaat gtgatgaaat aaggtaaaat
    10621 aacactgttt tctgtggata aacctgtgga tgttgtgcat aactgttggg gaaatctttt
    10681 taacaaaaat agccaaaata aacatataga cgaaaaaatt gattataata aacaaagtga
    10741 gcatgaagac caaaggagag aaaacatgaa tatcaaaatt atctcagtag gaaaattaaa
    10801 agaaaaatac ctgatccaag gtatcaatga gtatgtcaaa cggctgaatg cctatgcaaa
    10861 aatcgaattg atcgaagttc ctgacgaaaa agccccggag aatttaagcg aagcgcaaat
    10921 gagacaagta aaagaaaaag aaggggaacg gatcttagcg aaaatcaaag aacaagaata
    10981 cgtctatgcc ttagcaattg aagggaaaaa tcctacaagt gaatcttttg ctaaacaaat
    11041 cgaccaatta ggtatccaag gaaaaagcca ccttgttttt gttatcggcg gctctttagg
    11101 actcagcgaa gccgtcatga aaagaagcaa tgctcagatc tcttttggaa aaatgacgta
    11161 tccacatcaa ttgatgcgtc tgatccttgt cgagcaaatc taccgtgcct tccggatcaa
    11221 tgcgggggct ccataccaca aataatgata ttttttaaga gaaaaaatta tccctatttt
    11281 aaataatagc aataggtagt ggtagggaga aatttataaa taactttttt atttgctttg
    11341 gtagccaagt aagttgcaaa agaaaaatag atactgttaa aaagataatg
//
LOCUS       DACDKM010000088        11274 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20834_2016
            SAMN12640370-rid9891663.denovo.088, whole genome shotgun sequence.
ACCESSION   DACDKM010000088 DACDKM010000000
VERSION     DACDKM010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640370
            Sequence Read Archive: SRR10040891
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11274)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11274)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742835.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742835.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:04:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,012
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 29 of 75
            Pseudo Genes (incomplete)         :: 33 of 75
            Pseudo Genes (internal stop)      :: 19 of 75
            Pseudo Genes (multiple problems)  :: 5 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11274
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640370-rid9891663.denovo.088"
                     /isolate="E20834_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            236..1495
                     /gene="lysA"
                     /locus_tag="IV334_002137"
     CDS             236..1495
                     /gene="lysA"
                     /locus_tag="IV334_002137"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HAP8944193.1"
                     /translation="MTELIIGGVTASTLAEKYKTPLYVYDEEKMRQTMLSFKRGFVSK
                     RFDTKVLYASKAFQTIEMLHLVREYGFGLDVVSGGEIYTALQADFPRDTIYFHGNNKT
                     PEELIYALENDLLHIVADNLMEVELLAELSQRYLKKICIMLRLNVGVEAHTHEYIVTA
                     HIDSKFGMSYESKECQQALQLIKESSYLELEGFHAHIGSQIFDVTAWLAEIDKLTNYL
                     TDFDQPLSLNLGGGFGIRYTQNDQPIPVEEALKKLIEYTEEALEKRKLTIRQLLIEPG
                     RSIVGEAGTTLYTIGFIKKTPHKKYYFVDGGMTDNIRPALYQAEYDCDLAAKLSEEKT
                     EKVTIAGKMCESGDVVIKEAYLPKAESGDLLAVYSTGAYGYSMSSNYNRATRPAVVFV
                     NNGQSRLIVRRQAFSDLLRGEVSYDPS"
     gene            1482..2282
                     /gene="dapF"
                     /locus_tag="IV334_002138"
     CDS             1482..2282
                     /gene="dapF"
                     /locus_tag="IV334_002138"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HAP8944194.1"
                     /translation="MILHKYNGCGNDFILLDYAEDTDYSQLAASLCTKEKYDTDGLIA
                     VKIDPLEMIYYNKDGSRAPMCGNGIRCFARYVNDQNMMNEQEFNVETLAGTRKITVLD
                     PSPFYCSVDMGVPDDSPEKIKANQTYPIKNQEIEINGEKVTITSLFLGTIHTVVFVEN
                     AREEVWRKRGELICHHPLFKEKTNVNFVEVVNEQELIVRTYERGVGWTLACGTGCCAS
                     YVVAKETGKISADQVVVHLEQGDLMISGEDAIQMAGPAVHEWTRILEE"
     gene            2285..3166
                     /locus_tag="IV334_002139"
     CDS             2285..3166
                     /locus_tag="IV334_002139"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HAP8944195.1"
                     /translation="MIKGSIVALITPMNEEGSVDYAGLEKLIQFHLDEQTDGLLVLGT
                     TGESSTLTQSEEEQILQLTVKKVAGRVPVIAGAGTNNTKETIEKAKHFASLGADALLV
                     ITPYYNKTSDAGLAAHFTAIAEASPIPLILYNVPSRTGMSIPIHVLVNLAEHPNIIGL
                     KEASGDMAYVMDAARLIGEEFFLYSGNDDLILPVMSVGGSGVISVWANIQPKIVHELV
                     KDTQDGRWQQAKEKQLNALELIHALFSETNPIPVKAAMSLLDLPSGPLRLPLVSLSKE
                     KKKQLAQLLLKERTVEK"
     gene            3163..3879
                     /locus_tag="IV334_002140"
     CDS             3163..3879
                     /locus_tag="IV334_002140"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HAP8944196.1"
                     /translation="MKIGLIGSGQMGNRVEEVAQERGDTVLLLRTAPKEATTLAFTEL
                     PELLIDFSHPDNLEKILSYSLSYQLPLVIGTTGYTQSQFQEIKEHAKHVPVMYSANFS
                     FGIMVMNQLIKQAAAMLQGWQIELIEKHHSRKKDAPSGTANMLLQTIQEVQKLQPVYN
                     WQGKKREENQIGVHSIRAGSLPGEHDVLFAATDEIFTIKHESFSNRIFAAGAVEAADW
                     LKDQSPGYYKLEDLLMDKGV"
     gene            3885..4580
                     /gene="dapD"
                     /locus_tag="IV334_002141"
     CDS             3885..4580
                     /gene="dapD"
                     /locus_tag="IV334_002141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-acetyltransferase"
                     /protein_id="HAP8944197.1"
                     /translation="MEAQEIIRYIQTASKKTPVKVYLNVTEPIEFKESKVFGEGSSFV
                     VFGDYETIAPVLEAESEKIIEIEIETAARYSAVPLLDIKKINARIEPGAIIRDQVSIG
                     NNAVIMMGAIINIGAVIGENTMIDMGAVLGGRATVGKNCHIGAGAVLAGVIEPASAKP
                     VIVEDGVLVGANAVIVEGVHIGKDAVVAAGAVVLEDVAAETVVGGIPARVLKISDDQT
                     KENTALIAALREL"
     gene            4596..5597
                     /locus_tag="IV334_002142"
     CDS             4596..5597
                     /locus_tag="IV334_002142"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HAP8944198.1"
                     /translation="MREIDTCIVGATGLVGQTMLKVLEEYDFPVGRLKLLASKRSAGK
                     VCVFKKKKHTVQELTETSFEGYDLALFSAGASVSKEFAPIARSQGLFVIDNSSAWRED
                     SDIPLIVPEVNLEDYTLNRLIANPNCSTIQAVLPLKALQEAFGIQRVNYSTYQAVSGS
                     GQKGIEDLHRTQNDEEPVLYPYNISQTVIPEIDQPLENGYTKEEQKMINETRKILHQP
                     ALPVSATCVRVPIENGHGVSVAVQLQKEFTTEEIRECLSQFPGIQLEDDLAAHKYPTS
                     ILARGTDMVYVGRIRKDTSLENGLLFYTTADNIRKGAAANAVQIAAALMQKIAEVAR"
     gene            5594..6955
                     /locus_tag="IV334_002143"
     CDS             5594..6955
                     /locus_tag="IV334_002143"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HAP8944199.1"
                     /translation="MSNTLKVAKFGGTSMSHEEQFKKVRTIVLSDPNRQVIVVSALGK
                     RTSDDDKVTDLLYLIYAYLQHHVDWTPLWKRICDRFIQVRDVLALQLPIEDELARIQK
                     QLEDEKITEDYLVSRGEYLTAQLMAEYLGYQFIDAKDLIFFDYDGKIDQEKTAQEITK
                     AFKRYPKMVVPGFYGANPAGKEKLLGRGGSDITGAILAKMLQAESYENWTDVSGIMMA
                     DPRIIDHPKIIEEISFRELREMAYMGANVLHEAAVFPVQEADIPIQIKNTNDPFAQGT
                     KISNHEIEKENGLTGIAGRKDFLSITLFKRHMSDEIGFIWKAMSIFAKHEISIEHIPS
                     GIDNIGVIVSAEAIADKLFLITKELKETLGVEEIVVIEDLALISVVGGPHKELIGLSG
                     KVLSILNKLEIRTSILSQGAQELNLIIGVPNNQYETVVKGIYEGMVNTDAERISNGTP
                     SNS"
     gene            6915..8066
                     /locus_tag="IV334_002144"
     CDS             6915..8066
                     /locus_tag="IV334_002144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HAP8944200.1"
                     /translation="MLKEYRMALHQIPEIGFHEFKTKKYLYEQVKNSGAVIHEIGETG
                     LLLYFDNQQEETIAFRTDIDALPVKEETNLSFASKHEGYMHACGHDGHMAMLLGLTDF
                     ITKHIEDLIYNVVLIYQPSEEISGGAQSVIQSGLLDKYEVQAIFGFHLWPGLAKGEVF
                     SRPGPLMAQSSETDIIIKGQSAHIASSEKGIDSLEAAVRFLKNVYDFDRQLPAEWNHL
                     MKFGQIQGGTIRNVLAGEVIVSGSIRSYSSDMQNYLKRGLDHLAEQFHSTATISFRYN
                     DGYPAVINHRGLYAALLDSNKLHELDAPVLQAEDFGIYTQQYPCVFFFLGVGDTPALH
                     DPTFDFDMDVLENGVEWYKTILFTENLLVKIDSSSKEERVNNDEFIQSI"
     gene            8041..9210
                     /locus_tag="IV334_002145"
     CDS             8041..9210
                     /locus_tag="IV334_002145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HAP8944201.1"
                     /translation="MMNLSNRFSPRLSRIEVSKIRQFDQQISSIPDVIKLTLGEPDFP
                     TPEHVKQAGIAAIEEDFSHYTGMRGLEELREAACIFQQQRYGLTYDPQTEVLTTVGAT
                     EAIASALLSVLEEGDKVLIPAPAYSGYQPLVELAGAELIPIDTSDTGFVCQPEQFERA
                     FEQYGSAVKAVILNYPNNPTGTTLSAKQLKAIAEVLKKYPVFVISDEVYAELTYSGTH
                     MSIATYLPEQTIVISGLSKSHAMTGWRVGFIFAQKALIDELIKVHQYLVTAATTMSQK
                     AAAEALINGVKDSENMKEQYQTRRDYLVQQLAPLGFEVTQPNGAFYLFCKLPAAIQTD
                     SWQFCVELAEQAKVACIPGIAFGPEGEGYIRISYASSMEKLHEACKRIKDFLTEQ"
     gene            9385..10764
                     /locus_tag="IV334_002146"
     CDS             9385..10764
                     /locus_tag="IV334_002146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HAP8944202.1"
                     /translation="MEKIKVVIVGASHGGHQSILELLTRYGENVDITLFEAGDYISFM
                     SCGMELYLEDKVTDVNDVRNFRPENFPQSNVHILNNHEVKTIDADKKTVTVTSASDNE
                     TKEYPYDKLILSSGVKPNNLPVPGADLENVYLMRGYNWATKIKEKLNDPAIKKIAVVG
                     AGYIGIEAAEASRKAGKEVVLLDVIDRPLGTYLDAEMTDILEQHLNENGIEVLTNAKI
                     EAFTGNGKVEAIKTSEKEIPADLVIQAAGVKANTEWLKGIVDLDERGWIQTNEYLQTN
                     LPDVYAVGDATLAYSIPARKKMPIALATVARREARYVVKHLFEEVPNKPFGGVVGSSA
                     LSVFDYHFAASGLNSFTAAKAGVDVRVSYYEDTLRPKYVPEEQGNPKVSIQLFSDPMT
                     HQLLGGAVLSTYDVTGQGNVLALAIQNKLTLEELAEADFFFQPGFDRQWSMLNLAAQQ
                     ALGEEPFVE"
     gene            11045..11274
                     /locus_tag="IV334_002147"
                     /pseudo
     CDS             11045..11274
                     /locus_tag="IV334_002147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611166.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         3753 a         1959 c         2509 g         3053 t
ORIGIN      
        1 aaaatatttt ttcatcacta aatagtgttg aatttatgtt tttatgtata aatagcttct
       61 aatagttcgg gtattttgtt tttataacca tttttattct tatttttaaa aaaatatttc
      121 gaaatatact agacaattca gaaaattttg tttaatatta ttccaataaa attcaaatct
      181 ttatcaaatg aaattccaat aaaaacggaa aaaaagtgaa aagaggaatg aaaaaatgac
      241 agaattgata attggcggag tcacagcaag cacattggca gaaaagtata agacacctct
      301 gtatgtgtat gatgaagaaa agatgagaca aacgatgctg tcattcaagc gaggctttgt
      361 atcgaaacgt tttgatacaa aagtgcttta cgcctctaaa gcattccaaa cgattgagat
      421 gctgcattta gttcgtgaat atggattcgg gctagatgtt gtcagtggtg gggaaatcta
      481 taccgctttg caagctgatt ttcctagaga tactatctac ttccatggta acaacaagac
      541 gccagaggaa ctgatctatg cacttgaaaa tgatttgttg catattgttg ccgataattt
      601 gatggaagtt gaattattag cagaattaag ccaacgctat ttaaaaaaaa tctgtatcat
      661 gcttcggttg aatgtcggag tagaagcaca tactcatgaa tatatcgtca ctgcgcatat
      721 tgattcaaaa ttcggcatga gctatgaaag taaagagtgc caacaagccc ttcagttgat
      781 aaaagaaagc agctatctag agttagaagg atttcatgct catatcgggt cacaaatttt
      841 tgatgttact gcttggttag ctgaaatcga caaattgacg aattacttaa ccgattttga
      901 ccaacctctt tccttgaatc ttggcggagg gtttgggatt cgttatacac aaaatgatca
      961 gccgatccca gtcgaagagg cactaaaaaa actgattgaa tatacggaag aagctttgga
     1021 aaaacgaaaa ctgaccattc gtcaattact gatcgaaccg ggtagaagta ttgttggaga
     1081 agctggtaca acactataca cgattggttt catcaaaaag acaccacata aaaaatatta
     1141 ctttgtagat ggcgggatga cagataatat ccgtccagct ctgtatcagg cagaatacga
     1201 ttgtgattta gcagcaaaac tgtcggaaga aaaaacagag aaagtgacga ttgcaggaaa
     1261 aatgtgtgaa tcaggcgatg tggtaattaa agaagcttat ttgccgaaag cagaatcggg
     1321 tgatctttta gctgtttatt ccacgggagc ttatggatac tcgatgagca gcaactacaa
     1381 tcgagcgaca cgaccagcag ttgtttttgt caataatggc caatcaagac tgattgttcg
     1441 cagacaagca ttttctgatc tactaagggg ggaagtttcc tatgatcctt cataaatata
     1501 atggctgcgg aaatgatttt atcttattag attacgcaga agacacagat tatagtcaat
     1561 tagccgcttc tttatgcaca aaagaaaaat atgatacaga cggattgatc gccgtaaaaa
     1621 tcgatccgtt ggaaatgatc tattacaaca aagatggcag ccgcgcgccg atgtgcggaa
     1681 atggcattcg ctgttttgct cgatacgtaa atgatcagaa catgatgaat gaacaggaat
     1741 tcaacgtaga gacattagca ggcacaagaa aaattacggt gttggatccc tcaccttttt
     1801 attgcagcgt agatatggga gtgccagatg attctccgga aaaaatcaaa gctaatcaaa
     1861 cttatccaat aaaaaaccag gaaatagaga tcaatggtga aaaagtaacg atcactagtt
     1921 tatttttagg aacgattcat acagttgttt ttgttgaaaa tgcgcgtgag gaagtctgga
     1981 gaaaaagagg tgaactgatc tgtcatcatc ctttgttcaa agaaaaaacc aatgtcaact
     2041 ttgtagaagt agtcaatgaa caagaattga tcgtccgtac ctatgaaaga ggagtaggtt
     2101 ggacacttgc ttgcggtaca ggttgctgtg cctcttatgt ggttgctaag gaaactggga
     2161 aaatttcagc agatcaggta gttgttcact tggaacaagg agacttgatg atctctggtg
     2221 aggatgcgat acaaatggct ggcccagcag ttcatgaatg gacaagaata ttggaggaat
     2281 gaagatgata aaaggttcga tcgtggcgct gatcacgccg atgaatgaag agggatcagt
     2341 agattatgct ggtttggaaa aattgatcca attccattta gatgaacaaa cagatggatt
     2401 attagtttta ggtacaacag gagaatcttc tacgctcaca caatctgagg aggagcagat
     2461 ccttcaactg acagtaaaga aagtggctgg ccgtgtgccg gtgattgcag gagctggaac
     2521 gaataatacg aaagaaacga ttgaaaaagc gaagcatttt gcttctttgg gggcagacgc
     2581 acttttggtc atcacccctt actacaacaa aacaagtgat gcaggactag ctgctcattt
     2641 tacagcaatt gcagaagcct caccgattcc gcttattttg tataatgttc cttctagaac
     2701 aggcatgtcg attccaatcc atgtattagt aaatttagca gaacatccta atatcattgg
     2761 tttgaaagaa gcctcaggag atatggctta tgtgatggat gccgctcggt tgattggaga
     2821 agaattcttc ctttacagtg gaaatgatga tttgatccta ccagtaatga gtgtaggagg
     2881 aagcggtgta ataagtgtat gggcgaatat ccaacctaaa atagtccatg aattagtaaa
     2941 agatacgcag gacggaagat ggcagcaagc aaaagaaaag caattaaatg cacttgaact
     3001 gatccatgcc ttgttcagtg agaccaatcc tattcctgtc aaagcagcca tgtcattatt
     3061 agacttacct tccggacctc tacgcctgcc tttagtttct ctttcaaaag agaaaaagaa
     3121 acaattagct cagcttttgt taaaagaaag gacggttgaa aaatgaaaat cggactgatt
     3181 ggcagtggac agatgggaaa tagagtcgaa gaagttgcac aagaaagagg agatacggtc
     3241 cttcttttac ggacggcacc taaagaagca actacgcttg cttttacgga actgccagaa
     3301 ctgcttattg atttttctca tccagataat ttagagaaaa tcctttcgta tagcttatca
     3361 tatcaacttc cgttagtcat tggcacgaca ggttatacac aatcacagtt ccaagaaata
     3421 aaagaacatg ccaaacatgt acctgtgatg tatagtgcta atttttcgtt cggcatcatg
     3481 gtgatgaatc aactaatcaa gcaagcagct gcaatgttgc aaggctggca gatcgaactg
     3541 attgaaaaac atcatagtcg taaaaaagat gcaccatctg gtactgcaaa tatgctgctt
     3601 caaacaatcc aagaagtgca aaagctgcag cccgtataca actggcaagg gaaaaaaaga
     3661 gaagaaaacc aaattggtgt acacagtatt cgtgcaggtt ctttacctgg tgaacatgat
     3721 gtattgtttg ctgctactga tgaaatattt acgatcaaac atgaatcttt ttctaatcga
     3781 atctttgctg caggcgcagt agaagcagcg gactggttga aagatcagtc accaggttat
     3841 tacaaattag aagatttatt aatggacaaa ggggtgtaaa aaacgtggaa gctcaagaaa
     3901 tcatccgcta cattcaaacg gcatctaaaa agacgcctgt gaaagtctat ctgaatgtaa
     3961 ccgagccaat cgaatttaaa gaaagtaaag tttttggtga aggaagcagc ttcgttgttt
     4021 ttggtgacta tgagacgatt gctccggttt tagaagctga atcagaaaaa atcattgaaa
     4081 tcgaaatcga aactgctgca agatacagcg cagttccttt gctagatatt aaaaaaatta
     4141 atgctcgtat cgaaccaggt gctattatca gagatcaagt gtcaatcggc aacaatgccg
     4201 tgatcatgat gggagcgatt atcaatattg gtgcagtaat cggtgaaaac acgatgattg
     4261 atatgggtgc tgtcttaggt ggacgagcga cagtgggcaa aaactgccat attggtgcag
     4321 gagctgtctt agcaggtgtg atcgaaccag catcggccaa accggttatc gtagaagacg
     4381 gcgtactggt aggtgcgaat gcagtgattg ttgaaggtgt ccatatcgga aaagatgcag
     4441 tcgtggcagc aggagcagtg gttttagaag atgtggcagc agaaactgtt gtcggtggaa
     4501 tacctgcacg tgtattaaaa ataagtgatg atcagacaaa agaaaataca gcattgattg
     4561 ctgcgttacg cgaattatag aggagatgga gaaaaatgcg cgaaatagat acctgtattg
     4621 tgggggcaac cggattagtc ggccaaacga tgctcaaagt gttagaagaa tatgattttc
     4681 cagtaggacg attgaaatta ttagcttcaa aacgttcagc cggtaaagtt tgtgtattca
     4741 agaaaaagaa acacactgta caggaattga cagaaacttc atttgaagga tatgatttag
     4801 cgctattttc tgcaggtgct tctgtatcca aagagtttgc gccaatcgca cgaagtcagg
     4861 gactatttgt catagataat tcctcagcat ggcgggaaga ttcggatatt ccattaatcg
     4921 ttccagaggt caatctggaa gactatacat tgaatcggct gattgcaaat ccaaattgtt
     4981 caaccattca agcggtactt ccgcttaaag ctttacaaga agcctttggt atccagcgag
     5041 tcaattattc gacctatcaa gcagtttctg gttccgggca aaaaggcatt gaagatcttc
     5101 atcgtacgca aaatgacgag gaaccagttt tgtatcctta taatattagc caaacggtga
     5161 ttccggaaat cgatcagcct ttggaaaatg ggtatacaaa agaagaacag aaaatgataa
     5221 atgagacccg taagattctg catcagcctg ctttgccagt atcagcaacc tgcgtacgtg
     5281 tgccaatcga aaatggacat ggagtctctg tggcagttca actgcaaaaa gagtttacga
     5341 cggaagaaat tagagagtgt ctcagccagt ttccagggat tcaattagaa gatgatctag
     5401 cagcacataa atatccgacg agcattcttg ctagaggaac agatatggtt tatgttggga
     5461 ggatcagaaa agacacttct ttagagaacg gattgttgtt ctatacgact gccgataaca
     5521 tacgcaaagg agccgctgcg aatgccgtac agattgctgc agctttaatg cagaaaatag
     5581 cggaggtggc aagatgagta atacgctgaa agtagcgaaa ttcggcggaa catcgatgtc
     5641 tcatgaagag caattcaaaa aagtccgtac aattgttctt tcagatccca atcgccaagt
     5701 gatcgttgtt tctgctttag gcaaacgtac gtcagatgat gacaaagtaa cagacttact
     5761 ttatttgatt tatgcttacc ttcagcatca tgttgattgg acaccccttt ggaaaaggat
     5821 ttgtgatcgt ttcattcaag taagagatgt tcttgccctg cagttaccta ttgaagatga
     5881 actagcacgc atacagaaac aactggaaga cgaaaagata actgaagatt atttagtaag
     5941 tagaggtgaa tatctgactg ctcaactgat ggctgagtat cttggttacc agtttattga
     6001 cgcaaaagac ctgattttct ttgactatga tgggaaaatc gatcaggaaa aaacagcaca
     6061 ggaaatcaca aaagcattta aaagatatcc gaaaatggtt gttccagggt tttacggggc
     6121 gaaccctgca ggaaaagaaa aattattggg ccgtggggga agcgacatta ccggcgcgat
     6181 actggcgaag atgttgcaag cggaaagtta tgagaattgg acggatgttt caggaatcat
     6241 gatggctgat ccgcgaatca tcgatcatcc taaaatcatc gaggaaatct cttttagaga
     6301 actgagagag atggcttata tgggggctaa cgttcttcat gaagcagctg ttttcccagt
     6361 tcaagaggct gatatcccca tccaaataaa aaataccaat gatcctttcg ctcaagggac
     6421 aaaaatatcg aatcacgaaa tagaaaagga aaatggactg acaggaatag ctggacgcaa
     6481 ggattttctt tccatcactt tattcaaacg tcatatgtcc gatgaaatcg ggtttatctg
     6541 gaaagcaatg agcatttttg ctaaacatga gatatcaatc gaacatattc cttcagggat
     6601 cgacaatatc ggagtgatcg tctcagcaga agcgattgcc gataagttat ttctcatcac
     6661 gaaagaatta aaagaaacac ttggcgtgga agaaatcgtc gtgatcgaag acttagcttt
     6721 gatctctgtc gttgggggtc cgcataaaga attgattgga ttatctggga aagtgttatc
     6781 gatattgaac aaattagaaa ttcgcacatc gatcttatca caaggagcac aggagttgaa
     6841 tctgatcatc ggtgtaccga ataatcaata tgaaacagta gtgaagggta tctatgaagg
     6901 gatggtgaat acggatgctg aaagaatatc gaatggcact ccatcaaatt cctgaaatcg
     6961 ggtttcatga attcaagaca aaaaaatatt tatatgaaca agtaaaaaac agcggggccg
     7021 ttatccatga aatcggcgaa acaggactac tcctttattt tgataatcag caagaagaaa
     7081 cgattgcttt tcgaacagat atcgatgcac tgccagtcaa agaagaaacg aatctctctt
     7141 ttgcttctaa gcatgaaggt tatatgcatg catgtggtca tgacggacat atggcgatgc
     7201 ttttagggtt gactgacttt ataactaaac acattgagga tcttatttat aatgtagtac
     7261 tgatctatca gccatcagaa gaaatttccg gtggcgcaca aagcgttatt cagtcaggat
     7321 tgttggataa atatgaagta caagcaatat ttggctttca tctatggcct ggactagcta
     7381 agggagaagt tttctctcga ccaggacctt tgatggcaca aagcagtgaa acagacatta
     7441 tcattaaagg ccagtctgca catattgctt ctagtgaaaa aggaatcgat agtctggagg
     7501 cagctgtacg atttttgaag aatgtttatg attttgaccg gcaattaccg gcagagtgga
     7561 atcatctaat gaaatttggc caaatccaag gtggaacgat ccgcaatgtt ttagctggag
     7621 aagttattgt atccggaagt atccgttctt acagttcaga tatgcaaaat tatttgaaga
     7681 ggggattaga ccatttagca gaacagtttc actctacagc tactatttct tttcgctata
     7741 atgacggcta tccagctgta atcaaccata gaggcttata tgcagcactg ttagattcaa
     7801 acaaactaca tgaattagat gctccagttc ttcaagccga agattttggc atatatacgc
     7861 aacaatatcc atgtgtattt ttctttttag gtgtcggtga tacccctgct ttacatgatc
     7921 cgacgttcga ttttgatatg gatgttttag aaaatggtgt agaatggtac aaaacgattc
     7981 tttttacaga aaatcttctt gtaaaaatcg atagcagttc aaaagaggag cgagtaaata
     8041 atgatgaatt tatccaatcg atttagtcct aggctttcta gaatagaagt atcaaaaata
     8101 cgacaattcg atcagcaaat atcgtcgata ccagatgtga tcaaactcac cctgggagaa
     8161 ccggattttc caactcctga acatgtcaag caagcaggaa tagcagcgat tgaagaagac
     8221 ttttctcact ataccggaat gagaggatta gaggaactgc gggaagcggc ttgtatattt
     8281 cagcaacagc gttatggatt aacttatgat ccgcaaacgg aagttttgac gacagttggt
     8341 gcaacggaag cgattgcctc tgcgttgtta tctgttttgg aagaaggaga caaggttctt
     8401 attccagcgc ctgcttattc aggatatcag ccattagtgg aactagctgg tgctgaattg
     8461 atcccgatag atacttctga cacagggttt gtctgtcagc cggaacaatt tgaacgagca
     8521 tttgaacaat atggatcagc tgtcaaagca gtcattttaa actatcctaa caatcctact
     8581 gggacgacat tgtctgcaaa acaattgaaa gcaatcgctg aggttttaaa aaaatatccg
     8641 gtatttgtta taagtgatga agtatatgct gaattgactt atagtggaac gcatatgtcc
     8701 atagctactt atcttccaga acaaacaatc gtgataagcg ggttatccaa gtcacatgcg
     8761 atgactggtt ggcgagtggg ctttattttt gctcaaaaag cccttattga cgaactaatt
     8821 aaagtccatc aatatctggt gactgccgct acaacgatgt cgcaaaaagc tgcggctgaa
     8881 gcgctaataa atggggtgaa agatagcgag aatatgaaag agcaatatca aacacgaaga
     8941 gactatctgg tccagcaatt ggcgccttta ggatttgaag tgacccagcc aaacggtgca
     9001 ttttacttgt tttgcaaact cccagctgcc attcaaactg actcttggca gttttgtgta
     9061 gaattagcgg aacaggcaaa agttgcgtgt atcccaggaa ttgcatttgg cccagaagga
     9121 gaaggttata tccggatttc ttatgctagc agcatggaaa aattacatga ggcctgcaaa
     9181 cggatcaaag actttttaac agaacagtga ttcatagatt ttatcaaaaa aacaatcgtt
     9241 aattttgcgg tatattctgc aaggagacga ttgtttttta aatttagttc aaatgaacgc
     9301 gtttcttttg attcataact aatgtaatgt taagtttaaa ttgtttagaa tgattttaaa
     9361 agaatattca aggaggtttc atcaatggaa aaaatcaaag ttgtcattgt aggtgcatct
     9421 catggagggc atcaatcgat cttggaacta ttgacacgat acggtgaaaa tgtagatatc
     9481 acgctatttg aagcaggaga ctatatttcc ttcatgtcat gtggtatgga gctgtattta
     9541 gaagacaagg taacagatgt aaatgatgta cgtaatttca gaccagaaaa ctttccgcaa
     9601 tccaatgttc atattttaaa taatcacgaa gtaaaaacga tcgatgccga taaaaaaaca
     9661 gtaacggtta ctagcgcatc agataacgaa acaaaagaat atccttatga caaattgatt
     9721 ttgagttctg gtgtcaaacc aaacaatctg ccagtaccag gagcagatct agaaaatgtc
     9781 tacttgatgc gtggatacaa ttgggcaacc aaaatcaaag aaaaattaaa tgatccggct
     9841 attaaaaaaa tcgctgtcgt aggtgcgggt tacattggga tcgaagcagc agaagcaagc
     9901 cgaaaagctg ggaaagaagt cgtattgctt gatgtgatcg atagaccttt aggcacttat
     9961 ttagatgctg agatgacaga tattttagaa caacacctaa atgaaaacgg tattgaagtt
    10021 ttgacaaatg caaaaatcga agcttttact ggtaacggaa aagttgaggc aatcaaaaca
    10081 agtgaaaaag aaatcccagc agatctagtc attcaagctg ccggtgtgaa ggcaaataca
    10141 gaatggttaa aagggatcgt cgacttagat gaacgtggct ggatccaaac aaatgaatac
    10201 ttgcaaacga atcttccaga tgtgtatgca gtaggagatg ctacacttgc ttattctatc
    10261 ccagcacgca aaaaaatgcc gatcgcttta gcgactgttg cacgtagaga agcacgttat
    10321 gtagtaaaac atctttttga agaagtacca aacaaaccgt tcggcggagt cgtaggttcc
    10381 tctgcattaa gtgtctttga ctatcatttt gcagcaagcg gattgaatag ctttacagca
    10441 gcaaaagcag gagtagatgt acgagtgtct tattatgaag atacgttacg accaaaatat
    10501 gtaccagaag aacaaggaaa tccaaaagtg tccatccaat tattctcgga tccaatgacc
    10561 catcaattgc taggaggagc agtattatct acctatgatg ttacaggtca aggaaatgta
    10621 ttggcactag cgatccaaaa caaactgact ttagaagaac tagcagaagc tgacttcttc
    10681 ttccagccag gctttgaccg acaatggagc atgttgaatc tagccgctca acaagcgtta
    10741 ggagaagaac catttgtaga ataagaaaac gaaatatttg aaaagaggtt gtgaaaaagc
    10801 tgccttgcat caagtaataa gaacagccaa gtaaaaacag cttcttatgt ttttaacttg
    10861 gctggactta ttaccgcaga tgcaagcttt tgaacaccgt ttattttcaa aaaagggact
    10921 gtgacacgat ttgtgtaccg acccccaaaa gttagaccaa aaaatctaac ttttggaggt
    10981 cggtattttt tatggttaaa tatacttttg aacttaaatt aaagatcgtt catgattatt
    11041 tggatggcaa aggcgattgg accgtaaaaa tccagatcaa gagattgaag cggaaatgct
    11101 taaaattcgg gagaagcaca aggattatgg ctgtttacgg atgaccaatg aactaagaaa
    11161 ccgcggtttc tctatcaata aaaagaaagt tcagcgcttg atcaagaagc taggaataaa
    11221 agtaacggca tacacgcgta aatcacgtcg ctacaattct tatcgaggaa aagt
//
LOCUS       DACDKM010000089        11254 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20834_2016
            SAMN12640370-rid9891663.denovo.089, whole genome shotgun sequence.
ACCESSION   DACDKM010000089 DACDKM010000000
VERSION     DACDKM010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640370
            Sequence Read Archive: SRR10040891
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11254)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11254)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742835.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742835.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:04:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,012
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 29 of 75
            Pseudo Genes (incomplete)         :: 33 of 75
            Pseudo Genes (internal stop)      :: 19 of 75
            Pseudo Genes (multiple problems)  :: 5 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11254
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640370-rid9891663.denovo.089"
                     /isolate="E20834_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(17..1246)
                     /gene="hflX"
                     /locus_tag="IV334_002148"
     CDS             complement(17..1246)
                     /gene="hflX"
                     /locus_tag="IV334_002148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HAP8944203.1"
                     /translation="MEEKVIIVGVETEANQRYFSESMEELVQLTNTASGEVVFTITQK
                     RPQVDRQTIIGKGKLQELVQQADAHEADLIIFNHELTPRQSQLITDAVGLPVIDRVQL
                     ILDIFAMRARSKEGKLQVELAQLEYLLPRLVGQGKTLSRLGGGIGTRGPGETKLETDR
                     RHIRNKIFAVKKELKEVEAHRERNRQKRKNSEIFQIGLIGYTNAGKSTILNLLTQADT
                     YSKDQLFATLDPLTKRWRFAEGFEITVTDTVGFIQDLPTQLIDAFHSTLEESQNMDLL
                     LHVVDASSPDRILQEQTVLKLMDELNMKEMPILTVYNKADQIDPAMFTPTLFPNVLIS
                     TQTKEGKMALIEAIKRQLMELMTPYTLSVPSNEGQKLSELRRQTMVLDEAYMEETNEY
                     EVKGFAKTNSKWLRKNQ"
     gene            complement(1248..2165)
                     /gene="miaA"
                     /locus_tag="IV334_002149"
     CDS             complement(1248..2165)
                     /gene="miaA"
                     /locus_tag="IV334_002149"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HAP8944204.1"
                     /translation="MEKVLVIVGPTAVGKTALSVELAQLFGGEIISGDSLQIYKNLDI
                     GTAKIKEEEMNNVPHHLIDVIEPTQRYSAADFQHSGRQLITEIANRGHLPIIAGGTGL
                     YIQSLLYDYQLGATEEANPEIRKKYETLAETIGSQALWNQLKEKDPAAAEKIHWNNQR
                     KVIRALEVLETTGHSITAPKEEPKQLYDYLMIGLDTKRSILYERINQRVDLMLAEGLV
                     AEARKVYELGEVQASQGIGYKEFFPYFEGKETLEEATEQVKLHSRRYAKRQLTWFRNR
                     LDAQWFDLISEPNQKSQIEQTIKKWLEAE"
     gene            complement(2169..2906)
                     /locus_tag="IV334_002150"
     CDS             complement(2169..2906)
                     /locus_tag="IV334_002150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HAP8944205.1"
                     /translation="MLQIYAHRGSSGTHPENTLPAFAEAVRVGADGIELDVHLSKDGY
                     LIVMHDEEVDRTTNGKGLIREKTLEELKKLNAGGKFSKEFPAAKVPELREVLGLLLQK
                     NYRGMLMIEIKTDQYEYPGIEEKLAETLKSRDWPFQHAYCSFHLDSLEKIALLEPEIQ
                     LDLLMSTSDKKIQLAAETPYIEGVHPKNTWLFEHPVQAADFPKAIRAWTINDEEEMAK
                     CQELKIDGIITDFPEKALRWKQSTKSR"
     gene            complement(2997..3524)
                     /locus_tag="IV334_002151"
     CDS             complement(2997..3524)
                     /locus_tag="IV334_002151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP domain-containing protein"
                     /protein_id="HAP8944206.1"
                     /translation="MIVGITGLIGVLTGAVVVYIGMILQMRHLENKEQRRRELELKVK
                     EIETLNQLNKKINEILQKRTTLMEEYVSFDAFDDCYITIDDFAYLQSFSAQNSFYLPN
                     YILDELFKNISHRKVILSPDETMKIGGYAYKGGRVILENFTDSLTEMINEKKAQLKTM
                     TRQPIGFFSKDDVWS"
     gene            3704..4297
                     /gene="clpP"
                     /locus_tag="IV334_002152"
     CDS             3704..4297
                     /gene="clpP"
                     /locus_tag="IV334_002152"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HAP8944207.1"
                     /translation="MNLIPTVIEQSSRGERAYDIYSRLLKDRIIMLSGQVTDDLANSI
                     IAQLLFLDAQDSEKDIYLYINSPGGSVTAGMAIYDTMNFVKADVQTIVMGMAASMGSF
                     LLTAGTKGKRFALPNAEIMIHQPLGGAQGQATEIEIAARHILQTRERLNKILAERTGQ
                     PLEVIEKDTDRDNYMTAEQAKAYGLIDEVMENSSSLN"
     gene            complement(4401..4886)
                     /locus_tag="IV334_002153"
     CDS             complement(4401..4886)
                     /locus_tag="IV334_002153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HAP8944208.1"
                     /translation="MERFIASNATVIGDVTLSEDVTIWYQAVLRGDSNWIKIGQRTNI
                     QDGTIIHVDHDAPVDIAENVTVGHQCMLHGCTIEKGALIGMGTTILNHAVIGENSLIG
                     AGSLVTEGKVIPPNVLAFGRPAKVIRPLTKEEIQKNKENIQHYVEIGRMHAQGKFKEQ
                     R"
     gene            complement(5039..5236)
                     /locus_tag="IV334_002154"
     CDS             complement(5039..5236)
                     /locus_tag="IV334_002154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8944209.1"
                     /translation="MEQFLNEKITEAKREVDRLTHVCQTKPFDSADFFENEFQIERLA
                     AQIEVYELILKKVIEENIDVQ"
     gene            complement(5331..7091)
                     /gene="efrB"
                     /locus_tag="IV334_002155"
     CDS             complement(5331..7091)
                     /gene="efrB"
                     /locus_tag="IV334_002155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_029485765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux ABC transporter subunit EfrB"
                     /protein_id="HAP8944210.1"
                     /translation="MKKTFSSLKRLARYIRPYRETFLLVILFTVLTVAFNVALPYVTG
                     LPTTEISRNIANGESINFSYIFKCLIWITVVGIGYCISQLLSGVLMTNVVQSAMHDLR
                     RDIEEKINRLPVSYFDKNQQGNILSRVTNDVDAVSGALQQAFIGIVNAILGITLAAAM
                     MFYIQPMMALISMIMIPTSIWISKRVINASQKYFQSMQNSLGELNGYVQENMTGFSVL
                     KVYGREKETLEGFKKVNHSLKYFGFRAAFISGLMMPLVQLTAYATYIGMAVLGSFYVI
                     TGAIVVGQLQAFIQYIWQISQPMGNVTQLSSVLQSASAATKRVFEILDEPEEKENKVD
                     ASLPETILGDVTFDHVDFAYDPKKPLIQDLNFEVKAGQTVAVVGPTGAGKTTLINLLM
                     RFYDVDKGAIRIDGIDTKSMSRSDVRSLFGMVLQDAWLYQGTIADNIRFGKLDATDYE
                     VVDAAKTANVDHFIRTMPDGYDMAINSEGDNVSLGQKQLLTIARAVVSDPKILILDEA
                     TSSVDTRLEALIQKAMDRVMEGRTSFVIAHRLSTIRDADLILVMDQGQIIEKGTHESL
                     LAQGGFYEKLYNSQFAEEAE"
     gene            complement(7088..8818)
                     /gene="efrA"
                     /locus_tag="IV334_002156"
     CDS             complement(7088..8818)
                     /gene="efrA"
                     /locus_tag="IV334_002156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux ABC transporter subunit EfrA"
                     /protein_id="HAP8944211.1"
                     /translation="MKLMWHYTMRYKKFLFFNFICVFGFILIELGLPTILARMIDVGI
                     RNDDFDYVKQQGIFMIVITVIGIILNILLGYFGSRITTNIVADIRDDLFEKIQSYSHQ
                     EYENLGVSSLITRTTNDAYQIMLFLQNILRIGFMAPMMFIVSLYMVMRTSVTLSLYVV
                     GALPLLLLAVVAIAKVSEPLSKKQQKNLDKINSILRENLSGLRVIRAFVNEKFEEKRF
                     AVVNENYASSSKSLFRLMAVAQPGFYFLFNIVMVLIIWSGAVQIDAGELRVGDLIAFI
                     EYIFHALFSFMLFASVFMMYPRAAVSAGRIQEALDAKPSIVEDPNGISDTKTKGYIEF
                     QNVTFAYPGHSQEPVIRNVSFTASPGETVAFIGSTGSGKSTLIQLIPRFYDVSEGKIL
                     IDGVDVRDYRLSKLRDKIGFIPQKALLFTGTIADNLRYGKEDATQEEMERAAEIAQAS
                     EFISRKPDGFNEHLSEGGSNFSGGQKQRLAIARAIIRRPEIYIFDDSFSALDYQTDAK
                     LRARLKKETTESTVLIVAQRVGTIMHADKIIVLNEGEVVGMGTHRELLEKCPIYYDIA
                     ASQLSKEELA"
     gene            9211..9423
                     /locus_tag="IV334_002157"
     CDS             9211..9423
                     /locus_tag="IV334_002157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-dependent RNA polymerase auxiliary subunit
                     epsilon family protein"
                     /protein_id="HAP8944212.1"
                     /translation="MIYKVYYQETKVRNPKREETKSLYIEAKSDVDARQRVEENTPYN
                     IEFVQLLEGSHLEYEKENADFSLVEF"
     gene            9425..11104
                     /locus_tag="IV334_002158"
     CDS             9425..11104
                     /locus_tag="IV334_002158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HAP8944213.1"
                     /translation="MKLSIKNNETGVFAIGGLGEIGKNTYGVQFQDEIILIDAGIKFP
                     EDDLLGIDYVIPDYSYIIQNLHKVKALVITHGHEDHIGGIPYLLKQANIPIYAGPLAL
                     ALITNKLDEHGLLRDAELHEINEDTIIRFRKTSVSFFRTTHSIPDALGIVVKTPSGNI
                     VATGDFKFDFTPVGEPANLHKMARIGEEGVLCLLSDSTNAEIPTFTKSEKTIGHSILK
                     IFSKIEGRIIFASFASNIFRLQQAADAAVATGRKIAVFGRSMENAIVNGQRLGYIKVP
                     DGTFVDAHEINRLPANETMILCTGSQGEAMAALSRIANGTHRQIQIQPDDTVIFSSSP
                     IPGNTTSVNHLINLLSEAGAEVIHGKINNIHTSGHGGQEEQKLMLRLMKPKYFIPVHG
                     EFRMLKVHASLAQDVGVPAENCFIMENGDVLALTSDSARPAGHFVAGDVYIDGSGIGD
                     IGNVVLRDRHLLSEEGLVLAVATVDIKKKEILAGPDILSRGFVYMRESGEMINEAQRI
                     LFHALRECLNGPDCSEYKLKEAMTGALQPFLFERTERHPMILPMVMTPDEE"
BASE COUNT         3265 a         2429 c         1971 g         3589 t
ORIGIN      
        1 ttaaaaaaat aaaattttat tggttttttc ttaaccattt tgaattggtt tttgcaaacc
       61 ctttaacttc atattcgttt gtttcttcca tgtacgcttc gtccaaaacc attgtttgtc
      121 ttctcaactc gcttaatttc tgtccttcgt tgcttggcac agaaagtgta taaggggtca
      181 ttagttccat cagctgacgt ttgattgctt cgatcaacgc catcttacct tctttcgtct
      241 gagtagaaat caatacattt gggaatagcg taggcgtgaa catcgctggg tcgatctggt
      301 ctgccttatt gtaaacagtc agtatcggca tttctttcat attcagttca tccatcaatt
      361 ttaagacagt ctgctcctgt aaaatacgat ctggagaact agcatcgact acatgtagca
      421 aaagatccat attttgactt tcttctaatg tagaatggaa agcatcgatc agttgcgtag
      481 gcaggtcttg gatgaacccg acggtatccg ttacagtaat ctcaaatcct tcagcaaaac
      541 gccatctctt agtcaatgga tctaacgttg caaacaattg atctttcgaa taagtatccg
      601 cttgtgtcaa tagattcaag atagttgact tcccggcatt cgtgtagcca ataagtccaa
      661 tctgaaaaat ctcgctattt tttctttttt ggcgattgcg ctctcgatga gcttctactt
      721 ctttcaattc tttcttaaca gcaaaaattt tattccgtat atgtcggcgg tctgtttcta
      781 actttgtttc acctggccct cttgttccga tcccaccgcc aagacgagat aatgttttcc
      841 cttgccctac taaacgaggc agcaggtatt ctaactgagc aagttcaacc tgcagcttcc
      901 cttctttcga acgagcacgc atcgcaaaaa tatctaagat aagctgcacg cgatcaatta
      961 ccggaagacc aacagcatcg gtaatcagtt ggctctgtct cggagttagc tcatgattaa
     1021 agatgatcaa atccgcttca tgcgcatccg cttgttgaac taattcttgt aatttccctt
     1081 taccgataat cgtttgccga tccacttgtg gtctcttttg tgtaattgtg aatacgactt
     1141 ctcctgatgc ggtattcgtc agctgaacta gctcttccat tgactctgaa aagtaacgct
     1201 ggtttgcttc ggtttctacc cctacgatga tcactttttc ttccatttta ttccgcctcc
     1261 agccattttt tgattgtttg ctcaatctga cttttttgat ttggctcact tataagatca
     1321 aaccactgtg catccaaacg gttacgaaac cacgtcaact gacgtttcgc gtatcttctc
     1381 gaatgtagtt tgacttgttc tgttgcttct tccaatgttt ctttcccttc aaaataaggg
     1441 aaaaattctt tatatccaat accttgactg gcctggactt ctccaagctc atacacctta
     1501 cgcgcttctg ctactaaacc ttctgctagc atcaaatcga ctctttgatt gattcgttca
     1561 taaagaatac tacgcttcgt atctaatcca atcatgaggt aatcatacaa ttgctttggc
     1621 tcttctttag gtgccgtgat actatgtcct gtcgtctcta aaacttccag agctcttatc
     1681 actttgcgtt gattgttcca gtggattttc tcagcagctg ccgggtcttt ttctttcaac
     1741 tgattccata atgcttggga accaattgtt tcagctaacg tttcatactt ttttctaatc
     1801 tcaggattcg cttcttctgt cgctcctaac tgataatcat atagaaggga ttggatatag
     1861 agacccgtac caccagctat gataggtagg tgccctcggt tcgcaatctc ggtaatcaat
     1921 tgtcgacctg aatgctgaaa atcagcggct gagtaacgtt gagtaggttc gatcacatca
     1981 atcaaatgat gcggaacatt attcatctct tcttctttga tttttgctgt accgatatcc
     2041 agatttttat agatttgcaa tgaatcacca ctgatgatct ctcctccaaa tagttgtgcg
     2101 agttccacac ttagcgctgt tttaccgact gctgtcggac cgacgatcac taatactttt
     2161 tccatatttt acctactttt cgttgattgt ttccaccgaa gagctttctc cggaaaatca
     2221 gttattattc catctatttt gagttcttga catttggcca tttcttcttc atcgttaatc
     2281 gtccatgcac gaattgcttt tggaaaatca gctgcctgga ctggatgttc gaacaaccaa
     2341 gtattttttg gatgtactcc ttctatataa ggggtttctg ctgctagttg gatcttttta
     2401 tcagaagtac tcatcaataa gtccagctgt atctccggtt ccagtaatgc gattttttcc
     2461 aaagaatcca aatgaaagct gcaatatgca tgttgaaaag gccaatctct actttttaat
     2521 gtttcggcta atttttcttc gataccggga tattcatact gatcagtttt aatttcaatc
     2581 attagcatcc ctcggtaatt tttttggagg agcagtccta acacttctct caattcagga
     2641 actttagctg ctggaaattc tttgctgaat ttgccccctg cattcaattt tttgagttct
     2701 tccaatgttt tttctcgaat cagcccttta ccgttagtcg tccggtccac ttcttcgtcg
     2761 tgcataacga tcaaataacc atctttgctt agatggacat ctagttcgat accatctgca
     2821 cctactcgta cagcttctgc aaatgcagga agcgtatttt ctgggtgggt gccgctgctt
     2881 ccgcgatggg catagatttg taacatcttt cttcactccg ttcagttcta ttgtatcatt
     2941 aagaaaaaaa gaaagcatta ccacgctctc atgtgcaacg gcaatgcttc aaaatatcag
     3001 ctccaaacat catctttcga gaagaaacca atcggctggc gcgtcatagt cttcaactga
     3061 gctttttttt cattgatcat ttcagtcaga ctatccgtaa aattctctaa aatcacgcgt
     3121 ccgcctttat aagcatatcc accaatcttc attgtttcat ctggagataa aatcactttt
     3181 cgatgggaga tattcttaaa tagctcgtct aaaatataat ttggcagata aaaactattt
     3241 tgcgctgaaa atgattggag atacgcaaaa tcgtcaatcg tgatataaca atcatcaaat
     3301 gcatcaaagc tgacatattc ttccatcagc gttgtccgtt tttgcaagat ctcgttgatc
     3361 tttttattta attgattcag cgtctcgatt tctttgactt tcaattccaa ttcacgtcta
     3421 cgctgttctt tattttccaa gtgacgcatt tgcagtatca tgccgatata gacaactaca
     3481 gcacctgtca gcactccgat aaggccagta ataccaacta tcaatctgtt gttcctcctt
     3541 gttttgtttc tctgcttatc ataccataaa gcgggtttat gcgactaatt tgcctaagtt
     3601 ttttatttga ccttttttga ccaaaaatgt atactacatt ttaggtatta gaaactaaat
     3661 tatggtacac tatttctaaa cgaaatcaag gaggaactct attatgaatt taattcctac
     3721 agtcattgaa caatcttcac gaggtgaaag agcttacgat atctactcac gtttattgaa
     3781 agaccgtatc atcatgttaa gcggccaagt aacagatgat cttgctaatt cgatcatcgc
     3841 ccaattgctt ttcttagacg ctcaagattc tgaaaaagat atctatcttt atatcaattc
     3901 tcctggtggt tcagttacag caggtatggc tatttacgac acaatgaact ttgtcaaagc
     3961 agatgtccaa acaattgtta tgggtatggc tgcttcaatg ggaagcttct tgctgacagc
     4021 tggtacaaaa ggtaaacgat ttgctttacc aaatgctgag atcatgatcc atcaacctct
     4081 tggcggcgct caagggcaag caactgaaat cgaaattgcg gcacgccata ttttgcaaac
     4141 acgcgaacgc ttaaacaaaa ttttagctga acgcactgga caaccgcttg aagtgattga
     4201 aaaagataca gatcgtgaca actatatgac agctgaacaa gcaaaagcat atggattgat
     4261 cgatgaagtc atggaaaaca gttcttcatt aaattaatct gtttcaaagg aagctttttt
     4321 aagctctctt atctaaacca aataaaaaag gatggtcaac tcaaactgac catccttttt
     4381 tattttatgg atgctactgc ctatctttgc tccttgaatt ttccttgcgc atgcatccga
     4441 cctatctcga cataatgctg gatgttttct ttgttttttt gtatttcttc ttttgtcaaa
     4501 ggacggatca ctttagctgg gcgaccaaaa gccaagacat taggaggaat aacttttcct
     4561 tctgttacta atgatccagc accaatcaaa ctattctcac caataaccgc atgattcaat
     4621 atcgtggtcc ccatcccgat cagcgcccct ttttcgattg tacagccatg tagcatgcac
     4681 tgatgtccta ccgtcacatt ctccgcaata tctacaggtg catcatgatc gacatggatg
     4741 attgtaccat cttggatatt cgttctttgt cctattttta tccaattact gtccccgcgt
     4801 aaaacagctt gataccagat cgtcacatct tctgacaatg tcacatcacc aatcactgtg
     4861 gcatttgatg ctataaaccg ttccatattt atttttctcc tttcattttt tgtagaaact
     4921 aatcctattg aataaaggta tcatagcata tatttatttc ataagaaaaa aaacaggtaa
     4981 aacctgctca ggccagatgt ctatttacag tatctggtaa ctgacgtttt acctgttttt
     5041 attgtacatc tatattttct tctattactt tttttaaaat taattcgtac acttctattt
     5101 gtgctgccaa gcgttcgatt tgaaactcat tttcaaaaaa atcagctgaa tcaaaaggct
     5161 tcgtctgaca gacgtgcgtt aaacgatcaa cttctctttt ggcttcagta attttttcat
     5221 ttaaaaactg ttccatctcc gtgtcctcac ttttcttgat aactttactt taccattatc
     5281 aagaatagag aacaaaaaat aagttttaaa ctgttttatt cttatatgac ttattctgct
     5341 tcctcagcaa attggctgtt atacagtttt tcataaaatc caccttgtgc caataaactc
     5401 tcatgcgttc ctttttcaat gatctgacct tgatccatca ctagaatcag atctgcatca
     5461 cggattgtcg ataaacggtg agcgatgacg aaacttgtcc gtccctccat cacgcgatcc
     5521 attgcttttt gaatcaacgc ttctaaacgg gtgtctactg aacttgttgc ttcatctaaa
     5581 atcaagattt taggatcgga gaccactgct cgggcaatcg tcagcaactg tttttgtcct
     5641 aaagacacat tgtctccctc agaattgatg gccatatcat atccatctgg catcgttcgg
     5701 ataaaatgat ctacgtttgc tgttttcgca gcatcaacta cttcataatc tgtcgcatca
     5761 agctttccga agcggatatt atctgcaatc gttccttgat aaagccatgc atcttgcaag
     5821 accataccaa atagcgaacg cacatcactg cgactcattg atttggtgtc gattccatca
     5881 atccttattg ctcctttgtc tacgtcataa aaacgcatca atagattgat caatgtcgtc
     5941 ttgccggcac cggtcggacc gaccacagct acggtttgac cagctttcac ttcaaaattc
     6001 aaatcttgga ttaatggttt cttcggatca tatgcaaaat cgacatgatc gaatgtcaca
     6061 tctcctaaaa tcgtttctgg cagtgaagca tctactttgt tttctttttc ttctggctca
     6121 tctaaaatct cgaatacacg tttcgttgcc gctgatgcac tttgcaaaac tgaagacaat
     6181 tgtgtcacgt tccccattgg ctgactgatt tgccaaatat attggatgaa tgcttgtagt
     6241 tgaccgacaa cgatcgcccc agtaataaca taaaagcttc ctagaacagc cataccaata
     6301 taagtagcat aggcagttaa ttgaactaga ggcatcatca agccagaaat gaatgctgca
     6361 cgaaacccaa agtatttcaa gctgtgatta acttttttga atccttccaa tgtttctttt
     6421 tcgcgtccat atactttcaa tacactaaaa cctgtcatat tttcttggac gtagccgttt
     6481 agctcaccaa gagaattctg catgctttga aaatatttct gcgaagcatt gatcactcgt
     6541 tttgagatcc agatcgatgt tggaatcatg atcatagaga tcagcgccat catcggctgg
     6601 atataaaaca tcattgctgc agccaacgta attcccaata tcgcatttac gataccaata
     6661 aaagcttgtt gtaaagcacc gctgactgca tctacatcat tcgtcacacg agacaaaata
     6721 tttccttgct ggtttttatc gaaatatgaa accggaagac gattgatttt ttcttctatg
     6781 tctcttctca aatcatgcat ggcactttga acgacatttg tcatcaaaac accagaaagt
     6841 aattgcgaaa tacaataccc tattccaaca actgtgatcc aaatgagaca tttgaatata
     6901 taagaaaaat tgattgattc gccattggca atattacggc tgatttctgt agtaggaagt
     6961 cctgtaacat atggcaaagc cacgttgaaa gccactgtca gaactgtaaa taggatcact
     7021 aaaagaaaag tctcacgata cggtctgata tatcgagcta aacgttttaa ggaagaaaag
     7081 gtttttttca tgctaattcc tcctttgata attgagaagc ggcaatatca taataaatcg
     7141 ggcatttttc caacaactcc cgatgcgtcc ccataccgac gacttctcct tcatttaata
     7201 cgatgatctt atctgcatgc atgatcgttc ccacacgttg tgctacgatc aatacagtag
     7261 attctgtcgt ttcttttttc agacgagcac gtaacttcgc atccgtttga taatctaatg
     7321 cagaaaagct gtcatcaaaa atatagattt ccggacggcg tatgatggct ctggctattg
     7381 ccaatcgttg tttttgtcca cctgaaaaat tggaaccacc ttctgataaa tgttcattaa
     7441 atccatctgg ttttcgagaa atgaattctg aagcttgagc aatctcagct gcacgttcca
     7501 tttcttcttg cgtagcatct tctttcccgt atcgcaaatt atctgcaatc gttccagtaa
     7561 atagcagcgc tttttgcgga ataaacccaa tcttatctcg caacttgctt agccgataat
     7621 ctctcacgtc tactccatcg atcaagatct ttccttcact cacatcataa aagcgaggga
     7681 tcaattggat caacgtagat tttccgctac cggtacttcc aataaaagca acggtctctc
     7741 ctggagaggc tgtaaaactg acatttcgta tcactggctc ttgcgaatgg cctggataag
     7801 caaatgttac attttgaaat tcgatatacc ctttagtttt tgtatcagat ataccatttg
     7861 gatcttctac aatcgaaggt ttggcatcta gggcttcttg gatacgtcca gctgaaacag
     7921 ctgctcttgg atacatcatg aacacactag caaataacat gaatgagaac aaggcatgga
     7981 atatatactc gataaacgca atcaaatctc ctacacgaag ctcgcctgca tcgatttgca
     8041 ctgccccgct ccagatgatc aataccatta cgatattaaa taagaaataa aaacccggtt
     8101 gtgcaacagc catcaaacgg aataagcttt ttgaactaga agcataattc tcattgacta
     8161 cagcaaaacg tttttcttca aacttttcat tgacgaatgc acgaatcaca cgcaatcctg
     8221 aaagattttc ccgcaagata ctgttgattt tatctaagtt tttttgttgc tttttagata
     8281 acggttcaga caccttagca atcgctacga ctgccaataa tagtaaaggt aatgcaccta
     8341 ctacatacaa actgagtgtc acacttgtac gcatgaccat gtacaaactg acaatgaaca
     8401 tcattggtgc cataaaaccg atacgtaaaa tattttgcag gaacaacatg atctgataag
     8461 catcgtttgt tgtacgagtg atcaatgaag atacgcctag attttcgtat tcctgatgcg
     8521 aataactttg gatcttttcg aacaaatcat ctcgaatatc tgctacgata ttagtcgtaa
     8581 tcctcgaacc aaaataacct aacaagatat tcaaaataat accgattaca gtaataacga
     8641 tcataaatat cccttgttgc ttcacataat caaaatcatc attgcggatt cctacatcga
     8701 tcatacgtgc taaaatcgta ggaagtccta attcaatcaa aataaaacca aatacgcata
     8761 tgaaattgaa aaacaagaat ttcttataac gcatggtata atgccacatt agtttcactt
     8821 tctcatctcc ttttaatctt ttgttacatt ttcattacta aaaatgatag cacaacgagc
     8881 ctattaatag aacacttctc tcataaaaaa gtcattatga ttatagcggg atgataaaca
     8941 ttgatttttt ctttcaatct agttgatttc tatacttatt tgctgtttta tgctagaata
     9001 acaattaatt gttgtatatt ttttatacag aaggtacggt tgaaaaaata tccgaacaga
     9061 taagcaaaaa cgaggcttgt ctctttgact cgaatccagt attctagtca aagcatatga
     9121 aaaaccaggt ggcttgtccc ctcgttttgt gttgtctaaa aaaatcaact agctaaatca
     9181 ctgctttacg cagataaagg agacaaaaca atgatctata aagtttatta tcaagaaaca
     9241 aaagtacgca accctaaacg ggaagaaaca aaatctcttt acattgaggc aaaaagcgat
     9301 gtagacgctc gccaacgtgt ggaagaaaat accccataca atattgaatt cgttcaactt
     9361 ctagaaggaa gtcatctaga gtacgaaaaa gaaaatgctg atttctcatt agtggagttt
     9421 taatatgaaa ctttccatca aaaataacga gacaggcgtc tttgctattg gtggattagg
     9481 tgaaatcggg aaaaatacgt atggggtaca attccaagat gaaatcattt tgattgatgc
     9541 cgggatcaaa ttcccggaag acgatttact tggaattgac tatgtcattc ccgactacag
     9601 ctacatcata caaaacttac acaaagtaaa agccttggtg attactcacg ggcacgaaga
     9661 ccacatcggc ggaataccct acttgctgaa acaagcaaat attcctatct atgctggtcc
     9721 tctcgcatta gcgttgatca caaacaaatt agatgagcat ggcttgcttc gagatgcaga
     9781 gctccatgag atcaatgaag atacgatcat ccgttttaga aagacttctg tcagcttttt
     9841 cagaacgacc catagtatcc cagatgcatt agggatcgtg gtgaaaacac cttctggaaa
     9901 tatcgtagcg actggagact tcaaattcga tttcactcct gttggcgaac cagcaaactt
     9961 gcataaaatg gctcgtatcg gggaagaagg agtcttgtgc cttttatctg atagtacaaa
    10021 tgctgagatt ccaacattta ctaaatcaga aaaaacgatt gggcattcta ttttgaagat
    10081 cttttcaaaa atcgaaggac gtatcatttt tgccagcttt gcttctaaca tcttccgttt
    10141 gcagcaagca gcagatgccg ctgtggcaac tggtcgtaaa atcgctgttt tcggacgaag
    10201 catggaaaat gccatcgtca acggtcaacg tttaggatat atcaaagttc cagacggcac
    10261 atttgtagat gcacacgaaa tcaatcgctt gcctgccaat gaaacgatga tcctatgtac
    10321 aggttctcaa ggagaagcga tggccgcgtt gagccgcatc gcgaatggta ctcaccgtca
    10381 aatccaaatc cagccagacg atacagtgat cttttcaagt tcacctattc ctggtaatac
    10441 aacaagtgtc aatcatttga tcaatttgct gtctgaagct ggtgcagaag tgattcacgg
    10501 aaaaatcaat aacatccata cttccggtca cggtggacaa gaagaacaaa aattaatgct
    10561 tcgtttgatg aagccaaaat attttattcc tgtccacgga gaattccgca tgttgaaagt
    10621 acatgcttct ctagcacaag atgtaggtgt tcctgcagaa aactgcttca tcatggaaaa
    10681 tggcgatgtt ttagcgttaa ctagcgattc tgcgcgtcct gctgggcatt ttgtggctgg
    10741 agatgtgtat atcgatggta gcgggatcgg tgatattggt aatgtcgttt tacgtgaccg
    10801 tcacctgctt tcagaagaag gtcttgtatt agctgtcgcg acagtggata tcaagaaaaa
    10861 agaaatcttg gctggtccag atattctctc tcgtggattt gtctacatgc gagaatcagg
    10921 agaaatgatc aatgaagctc agcgaatctt attccacgct ttaagagaat gtttgaatgg
    10981 acctgactgt tcagaatata aattgaaaga agcgatgact ggcgcgcttc agcctttctt
    11041 attcgaacgt acagaaagac atccaatgat tttgccaatg gtaatgacac cagatgaaga
    11101 ataaataaga ggttatgaaa tcagcacctt gtcctgaata taagagaaaa caaatgaaaa
    11161 acatgagaag ccatttttgt ttgttcttat tccttgatac aaggtggctc tttcatagcc
    11221 tcttaaattt atttactttt tcatacaaaa aaat
//
LOCUS       DACDKM010000090        11231 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20834_2016
            SAMN12640370-rid9891663.denovo.090, whole genome shotgun sequence.
ACCESSION   DACDKM010000090 DACDKM010000000
VERSION     DACDKM010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640370
            Sequence Read Archive: SRR10040891
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11231)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11231)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742835.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742835.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:04:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,012
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 29 of 75
            Pseudo Genes (incomplete)         :: 33 of 75
            Pseudo Genes (internal stop)      :: 19 of 75
            Pseudo Genes (multiple problems)  :: 5 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11231
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640370-rid9891663.denovo.090"
                     /isolate="E20834_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            6..305
                     /locus_tag="IV334_002159"
     CDS             6..305
                     /locus_tag="IV334_002159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAP8944214.1"
                     /translation="MKNRLIVACGSGVATSQTIASKIQSMLEDDGIAFPVEAVDYKSI
                     QNELLTAGIYVYVAQPDEEVLEQAKDLGIEVFPGIPFLTGMGVEPIYDSIKELVQ"
     gene            378..1739
                     /locus_tag="IV334_002160"
     CDS             378..1739
                     /locus_tag="IV334_002160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HAP8944215.1"
                     /translation="MEVFQTIINYVLNLGSAIFVPLIILILGLLAGMKLKKAFMSALT
                     LGIAFSGMSMVIGYMSNAVSPASEALAKNTGISLPALDLGWTGAASITWSWSYAFIFF
                     AVTIGVNFVLLLFNWTKTLNVDMWNVWGKALTAYLVYYVSGSLAAGFLTAMVQVILEL
                     KLGDMFQKHIQDLTGIPLVTVTHFMTSAAVLLLPFNMIMDKIPALNKRADTNALKKKI
                     GIFSENSVMGFIIGLGLGFAAAYGVSGSLNLAIQTATALSLFPMISKMFMQSLSPLAD
                     AMSEFMKKRFKDREVYIGLDWPILAGRSEIWVTAILLVPVFIGYAIILPGNQVLPLAG
                     IINYSIAVGGLLLTGGNLARMLILGIITMPIYLYGATYLAPILSDLAEKSGAVEGIKK
                     GQLITWSSIEGPEFRVLFAEAFNRNILAIVGCVIFLGLFVLLYKYMIKAKVPSERYAE
                     LQK"
     gene            1761..2021
                     /locus_tag="IV334_002161"
     CDS             1761..2021
                     /locus_tag="IV334_002161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8944216.1"
                     /translation="MKREETDKIKWTVALCGTLLLFLYGLFTQNIIINLLVIFFALVI
                     YKYGNHVLFREYDEKRKRKIEESMKIKEATKEILREKSFIKR"
     gene            2035..2787
                     /locus_tag="IV334_002162"
     CDS             2035..2787
                     /locus_tag="IV334_002162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase"
                     /protein_id="HAP8944217.1"
                     /translation="MSDGRMLFERERQDMARIVRVIFERKNTNVAGGNFSFKTTDDQG
                     KDYIIMTPTMMSEAYLGEISPAQVLVVEPRTRRIVDGVGHLTREINMHEAMYETNPAI
                     KAVLHAHAPNAMFWATTGLPMPNLTEATAKVKEIPVMDFEPNCSEELAELTSDYVKKQ
                     HLELPHMLLLNSHGVLINTGGESGIEAIHKALQILDTVEWNAEIAYKQTIFQALGITD
                     GFYSKGEKIGGLEELKHKTAIYNKSVIAAGGD"
     gene            complement(2881..3486)
                     /locus_tag="IV334_002163"
     CDS             complement(2881..3486)
                     /locus_tag="IV334_002163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAP8944218.1"
                     /translation="MTYIFEKGTSGKHLILLHGTGGDEHSLLDIAHFLAPNSTLLSFR
                     GTVQEDGMNRFFKRNGLNQFDLDSLEKETDKLHEKIKEISEKELIPLSDWLLVGYSNG
                     ANIAAHLLLERETELNKGLFFHPMSLGVHQSRSTLEDKTVWISYGDNDPIVSPASFKE
                     LADQFETRQANVTIQLTVTGHQLTMDEIKQAREWTDHLLTK"
     gene            complement(3539..4513)
                     /locus_tag="IV334_002164"
     CDS             complement(3539..4513)
                     /locus_tag="IV334_002164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ring-cleaving dioxygenase"
                     /protein_id="HAP8944219.1"
                     /translation="MDTQIRGIHHVTAMTSSATKIYRFFTDILGMRLIKKTVNQDDIE
                     TYHLYFTDDTGAPGTDMTFFDFPNQPKGKKGTDTISRASFRVPSDAALDFWLERFEKY
                     DVTHSAINERFGKKYIEFEDFDNQYYQLISDENNQGVAAGIPWKNSNVPTEFAITGLG
                     PVFVRVSQLEHIRLILEKILGFKETDHSGDFYLFEIGEGGNGASIIIEHRTDLPKAIE
                     GFGNIHHLALRVADKEALEYWIQVINKIELPNSGFVDRFYFQSEYFLAAHHVLFELAT
                     DGPGFLEDEPAESAGEKLSLPPFLEERREAIEAYVRPFDTSDANKKRE"
     gene            complement(4891..5670)
                     /locus_tag="IV334_002165"
     CDS             complement(4891..5670)
                     /locus_tag="IV334_002165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAP8944220.1"
                     /translation="MLLRVVGSIIFRYTLEEVNNVPKYEEIANILRERIRSKEYPLDS
                     FLPNQVDLVKEFGASRMTIKKAINILAMEGLVYSQRGSGTKILNHPFMQKDTSPANEY
                     EGLSLQMKRRHRSLKSQIIHFDVEFPNQYIQERLMIDAEQPVYNIHRLRILDGEPYVL
                     EHTFMPVHLVPGLKKEHLLASIYDHLHQELNVHFAGAYRAISADKSDEYDQQYLNCAE
                     NDPVLEVQQVVYLKDGQPIEYSSSRNRFDVRNYSVLDVRST"
     gene            complement(5737..7008)
                     /gene="celB"
                     /locus_tag="IV334_002166"
     CDS             complement(5737..7008)
                     /gene="celB"
                     /locus_tag="IV334_002166"
                     /EC_number="2.7.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIC"
                     /protein_id="HAP8944221.1"
                     /translation="MNGFMDKLSEKIMPLANLLGQNRYLTVLRDAFMLSFPLTMFGSI
                     VVVINNLPFFSDATKGTLSNLFGNGQNATMSIMSVFVTFGIGYYLSKSYDVEGIFGGA
                     VSFASFLILTPFAMKTAGGEDVTGVLSLDRLGAKGMFIGMIAAFLAGEIYCRITKRGW
                     QIKMPDGVPPAVTKSFAALIPAVVTLTIFLVINAVMTGIFNANLHDVVYEVIQKPLTG
                     LGSSLPATLIALFFVQFLWFFGLHGQIIVNSVMDPIWNTLMLDNLEAYQHGKELPHII
                     TKPFMETFTVGIGGSGMTLAVVLLMAFVLKKKQYRDVGRLALAPGIFNVNEPAIFGLP
                     IVLNATILIPWVVAPLIVTTFNYLVMAAGLVPAPTGVSVPWTVPIIASGVLATNSWLG
                     GMLQVIDFVIVAFIWYPFLKVLDKQPDLDIA"
     gene            7161..8558
                     /locus_tag="IV334_002167"
     CDS             7161..8558
                     /locus_tag="IV334_002167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HAP8944222.1"
                     /translation="MKTYTFPENFWWGSAASGPQTEGRVSDDGKGENIWDHWYELEPE
                     KFFDQVGPEKTSQVYTKYKEDVQLMKETGHNTFRTSIQWSHLIPEGTGEINQKAVSFY
                     RDYFTELLDNGIEPFVNLYHFDMPLPLQEKGGWLSRETVDAYVSYAAACFELFGDLVK
                     KWFTHNEPIVPVEGGYLYQFHYPNHINMKEAVQVGYHENLASAKVIKAYHEGGHDGEI
                     GIILNLTPSYPRDETNPEDVKAAKIADAFFNRSFLDPAVKGEFPQELIEIVKELAIMP
                     EIHEGDLELIRENTIDLLGINYYQPRRIKAKETPINHEHSPMPDDYFDNYDMPNKKMN
                     PYRGWEIYEKGIYDILTNTRENYGNIKCYISENGMGVEGEERFVNSEGIIEDDYRIEF
                     VTDHLKYVHQAIQEGTNCVGYHMWTCMDNWSWTNAYKNRYGFIAVDLAQDGKRTIKKS
                     GRWFKTVSEKNGFDA"
     gene            8639..9079
                     /locus_tag="IV334_002168"
     CDS             8639..9079
                     /locus_tag="IV334_002168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HAP8944223.1"
                     /translation="MVDTYRNILVAIDGSEKAEKAFLEAITIAKRNQATLHILYVNEV
                     TGNYFGDFAFVTTSLQEELDEVAENQMKEHRNLAIEKGLTDIETYVLYGYPKTLIANF
                     HESKEPIDLIVMGATGLNAVERALVGSTTSYVVNHASCNVMVVK"
     gene            9227..9553
                     /locus_tag="IV334_002169"
     CDS             9227..9553
                     /locus_tag="IV334_002169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HAP8944224.1"
                     /translation="MISSDGIRGYNDAIILSILNKNDSYGYEISKTIRERTNDSYVIK
                     ETTLYSVFARLEKNQLIVSYPGEVTHGKKRTYYKITEIGRNLLEEKKQEWLTTKDVVD
                     QFLEGN"
     gene            9554..10303
                     /locus_tag="IV334_002170"
     CDS             9554..10303
                     /locus_tag="IV334_002170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8944225.1"
                     /translation="MDTIRNYLDSLFIGVPQSTEIDKLKTDLLANMEDHYHELMGEGK
                     NEQEAIGTVISTFGSIDELLEELDVEKKHQADETETNTASIYLSEAENYWKEYRAASL
                     QVASGVLFISLSFASFLFFCSAGYVFMGISCLIFGIALAVGFFIASGMKITRLNHFLH
                     HRKIPEKVLAEAKEKEEEYQRSFGFSLIAGIGLCIFSLFPLLASLMWYMDGSIGASIF
                     FVTVGTGVFLIIYGSLVRHSYRQFTQSAYYW"
     gene            complement(10383..10838)
                     /locus_tag="IV334_002171"
     CDS             complement(10383..10838)
                     /locus_tag="IV334_002171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HAP8944226.1"
                     /translation="MLTIYSSRTHSYHLMANWLKDHQMPYRERIFTPKQPLTRKEIFY
                     LLSLTENGFDDLLAKRSIDYKNLKDQIDQCTTSELVDLIVSHFTLLQRPIITDGKKLL
                     VGFNEDSMRLFIPVQWRRSQLKSVYNDFTWVSNLENSTDLDDEFLNYYI"
BASE COUNT         3562 a         1960 c         2302 g         3407 t
ORIGIN      
        1 aaaaaatgaa aaatcggtta attgtcgcat gtggaagtgg agtagcaacg agtcagacaa
       61 tcgctagtaa gatccaaagt atgttagaag atgacggcat cgcatttcct gtagaagcag
      121 ttgattacaa atcgattcaa aatgagcttc taactgctgg aatctatgtg tatgtcgcac
      181 aacctgatga ggaagtactt gaacaagcaa aagatttggg tattgaagta tttccaggaa
      241 ttccattttt aactggaatg ggtgtagaac ctatttatga cagtatcaaa gagttagtac
      301 agtaaaggag gattgttgga taaaaaagtt ggttcatagt tttatttact atatttagaa
      361 agaaagaagg tttatttatg gaagttttcc aaaccattat caattatgtt ttgaacttag
      421 gatcagccat ttttgttccc ctgattattt tgatacttgg attgttggca gggatgaaat
      481 taaaaaaagc attcatgtct gctttgactt tagggattgc ttttagcgga atgtctatgg
      541 tcattggcta tatgtctaac gcagtaagtc cagcatcaga agcattggct aaaaatacgg
      601 gaatcagttt gcctgcactt gatcttgggt ggacaggtgc agcaagcatc acatggtcgt
      661 ggagttatgc ttttatattt tttgcagtaa cgattggggt caattttgta ctgttacttt
      721 ttaattggac gaagacgtta aatgttgata tgtggaatgt ttgggggaaa gctttaactg
      781 cttatttagt ttattatgtt tccggtagtt tggctgcagg ctttcttact gcaatggtcc
      841 aagttatatt ggaattaaag cttggtgata tgtttcaaaa acatatacaa gatctcacag
      901 ggattccatt agtaacagtg actcatttta tgacatctgc cgctgtcttg ttgctgcctt
      961 tcaatatgat catggataaa attcctgctt taaataaacg agcggatacg aatgcattga
     1021 agaagaaaat tgggatcttc tctgaaaact ctgtaatggg attcattatt ggattggggc
     1081 ttggctttgc tgctgcgtat ggcgtttcag gttctcttaa tctagcgatc caaacagcta
     1141 cagcattaag tttgtttcct atgatctcta aaatgttcat gcaatctttg tctcctctag
     1201 cagacgctat gtcagaattt atgaaaaagc ggtttaagga tcgtgaagta tatatcggat
     1261 tggactggcc gatattagct ggtcgttcag aaatttgggt tactgctatt ttacttgttc
     1321 ctgtgtttat tggttatgcc attattttgc caggaaacca agtattgcca ttagcaggga
     1381 ttatcaacta ttcgattgcg gtcggcggat tacttttaac aggaggaaac ttggctcgta
     1441 tgctgatctt gggaatcatc actatgccta tttatttgta cggggccact taccttgcgc
     1501 caatactcag tgatttagca gaaaaaagcg gagcagtcga gggaatcaag aaaggacagt
     1561 tgatcacatg gtcatccata gaagggccgg agttcagggt tttatttgcg gaggcattta
     1621 ataggaatat tctcgctatt gtaggatgcg tgatttttct aggactgttt gttctacttt
     1681 ataaatatat gataaaagcg aaagtaccga gtgaacgtta tgctgaacta caaaaatagc
     1741 attgaaatgg tgggaaagta atgaaaagag aagagacaga taaaataaaa tggacagtcg
     1801 ctctttgtgg tacacttctg ctatttctat atggactctt tactcagaat atcatcatca
     1861 atctattggt catttttttc gcactagtga tctataaata tggaaatcac gttttattca
     1921 gagagtacga tgaaaaaaga aaacggaaaa tagaagaatc aatgaaaata aaagaagcta
     1981 ccaaagaaat attgagagaa aaaagtttta taaaaagata atggaggggt cattatgtca
     2041 gatggacgta tgttatttga gagagaacga caggacatgg cacgtatcgt aagagtgatt
     2101 ttcgaaagga aaaacacaaa tgtcgcagga gggaattttt cctttaaaac cactgatgat
     2161 caagggaaag actacatcat catgacgccg accatgatgt cagaagccta tctaggagag
     2221 atttctcctg cacaggttct agtagtagaa ccaagaacgc gtagaatcgt tgacggagtc
     2281 ggtcatttaa ctcgtgaaat caatatgcat gaagcgatgt atgagactaa tccagctatc
     2341 aaagctgtcc tacatgctca tgcgcctaat gcaatgttct gggcgactac tgggttgcct
     2401 atgcctaatt tgacagaagc cacagctaaa gtaaaagaga ttcctgtcat ggactttgag
     2461 cctaattgct ctgaagaact tgctgaattg acaagcgact atgtaaaaaa acagcatctc
     2521 gaattaccgc atatgttgct gctcaactct catggcgtat tgatcaacac aggtggagaa
     2581 tcaggaatcg aagctatcca taaagcactg caaatccttg atacagttga atggaatgca
     2641 gaaattgcct acaagcagac gatttttcaa gcattaggga ttactgacgg tttttattca
     2701 aaaggtgaga aaatcggggg attggaagag ttgaaacata aaacggctat ttataataaa
     2761 tctgttattg cagctggtgg agactaacct atctgacaca aaaaactacg actttcacaa
     2821 aatctgatcc agatcgatat ctgcttcagg ttgtattatg taagtcgtag ttcttttctt
     2881 ttatttagtt aagaggtggt ctgtccattc cctcgcttgc ttgatttcgt ccatcgtcaa
     2941 ctgatgacct gttacagtta gctgaatagt cacatttgct tggcgtgttt cgaattgatc
     3001 ggccaattct ttaaaggaag caggggaaac gattggatcg ttatcaccat aagaaatcca
     3061 gacggtttta tcctctaatg tggaacggct ttgatgaaca cctagagaca tcggatggaa
     3121 aaacaaccct ttatttagct cggtctcacg ctccaataat aaatgagcag cgatgtttgc
     3181 gccatttgaa tagccgacaa gcagccagtc acttaacggt atcagttctt tttcgctgat
     3241 ttctttgatt ttttcatgga gtttatctgt ttctttttcc aaactgtcca ggtcgaattg
     3301 attcaatcca ttacgcttga aaaaacgatt catcccatct tcttggaccg tgccacgaaa
     3361 agaaagcaat gtactgtttg gtgcaagaaa gtgagcgata tccagtaaag aatgttcatc
     3421 gccacctgtg ccatgaagta agatcaaatg cttgccactt gttccttttt caaaaatgta
     3481 ggtcattttg ttccctcttt tctgtttcta tagatgcaaa tgaagtagca tgaagagatt
     3541 attcacgttt tttatttgca tcacttgtat cgaaaggacg gacataagct tcaatggctt
     3601 cacgacgttc ttctaagaat ggaggaagag atagtttttc tcctgctgat tctgctggtt
     3661 catcttcaag gaatccagga ccatcagttg ctaattcaaa cagtacgtga tgagcggcta
     3721 ggaagtactc agattggaaa tagaaacgat cgacgaaacc agaatttggc aattcaatct
     3781 tattgattac ttgaatccag tattccaatg cttctttgtc tgctacacgc aatgctaagt
     3841 gatggatatt tccaaatcct tcgattgctt ttggcaagtc tgtccgatgt tcaataatga
     3901 tgcttgcacc atttccccct tcaccgattt caaataagta gaaatctcct gaatggtctg
     3961 tttctttgaa tcctaagatt ttttcaagaa tcaagcggat atgctctaat tgactgacac
     4021 ggacaaagac aggtccaagt cctgtgatcg caaactctgt cggtacattg ctgtttttcc
     4081 aaggaatgcc agctgctaca ccttgattgt tttcgtcaga gatcaattga taatactgat
     4141 tatcgaaatc ttcaaattcg atatattttt tgccgaaacg ttcattgatt gctgaatgag
     4201 taacgtcata tttttcaaaa cgttccaacc aaaagtctaa tgctgcgtca cttggcacac
     4261 ggaaagaagc acgagagatc gtatctgttc ctttttttcc ttttggctga tttggaaaat
     4321 caaagaaagt catatctgtt cctggcgcac cagtatcgtc tgtaaagtaa agatggtaag
     4381 tttcgatgtc atcttggtta actgtttttt tgattaagcg cattcctaaa atatctgtaa
     4441 aaaagcggta gatttttgta gcgctagatg tcattgctgt gacgtggtga attcctctga
     4501 tttgtgtatc catgataaat ttcctcctgt taacttacaa aaagtaagta gttgatttag
     4561 acatagtata gagggtgcat ctctattttg caattaattt gtcttctaaa gtatcaggaa
     4621 ggaatcaaga atggaaaaga ggagggacgg atcaataaaa taaaaagact tacgactagc
     4681 tgtatcgatt tcaaaaaaac tagctttaaa aagctaactt tagaatcatt acgggtggtc
     4741 ataagtcttt ttatgctgag ttattttttc atttttgagc taatgcttag gatagaatag
     4801 aatgttgatt tcacgatctc ttcttgttac ttgaaactgc ttaccactgt cacgatctct
     4861 tggtaaacgg ctttttcaca acctctttgg ttaagttgaa cgcacatcca gcacagaata
     4921 gttccgaaca tcaaatcggt ttcggctgga agaatattca atcggctgtc catctttgag
     4981 atagacgact tgctgaactt ccagtacagg atcgttttct gcacagttca aatattgctg
     5041 atcatattca tcacttttat ctgcagaaat agcacgataa gcccctgcaa aatggacatt
     5101 caattcttga tgaagatgat catagataga ggctaacaaa tgttcttttt ttaaacccgg
     5161 cacaagatgg actggcataa aggtatgctc taaaacatat ggctcgccat ctaagatcct
     5221 taaccggtga atattgtaga caggttgctc ggcatcgatc atcaatcgtt cttggatata
     5281 ttgattaggg aattcaacat caaaatgaat gatttgactc ttcaaagaac gatgtctgcg
     5341 tttcatttgg agactcaaac cttcatactc gttagcgggc gatgtgtctt tttgcataaa
     5401 tggatgattc aagattttcg ttcctgatcc tcgctgcgaa tagacaagtc cttccattgc
     5461 taagatattg atggcttttt tgattgtcat ccggctcgca ccaaattctt ttaccaagtc
     5521 gacttgatta ggcaaaaaac tatcgagagg atactctttt gaacggatac gttctcttaa
     5581 aatatttgcg atttcttcgt attttggcac gttattcacc tcttctagag tataacgaaa
     5641 gattatgctt ccgacaactc gaagtagcat ctggaacatt gtttattcga aaaaagaggt
     5701 catgacaagt ttttgtcaca accccttaat gatctattaa gcaatatcta aatctggttg
     5761 tttatctagt actttcagga atgggtacca gataaaggca acgatgacaa agtcgatgac
     5821 ttgcagcata ccaccaagcc atgaatttgt tgctaagaca ccgcttgcaa tgattggcac
     5881 agtccatgga acagagacgc cagttggtgc agggacaagt ccagcagcca ttaccaaata
     5941 attgaacgtc gttacgatta gaggtgccac aacccaagga ataaggattg ttgcattcaa
     6001 tacgatcggt agaccgaaga tcgctggttc atttacattg aaaataccag gtgctaaagc
     6061 taatcgtcca acatcacgat actgtttctt tttaagaaca aaggccatca ataaaacgac
     6121 agctaaagtc attccagagc caccaatacc aacagtgaag gtttccatga atggttttgt
     6181 gatgatgtgt ggtaattctt ttccatgttg ataagcttct aagttatcaa gcatcagtgt
     6241 attccaaatc gggtccatta cagagttgac gatgatttgt ccgtgaagtc cgaagaacca
     6301 taagaattgg acaaagaaca aagcaatcaa tgttgcaggt aaactacttc ccaaaccagt
     6361 taatggtttt tggatcactt cataaacgac gtcatgtaag ttagcattga aaataccagt
     6421 catcacagca ttgattacta agaaaatagt tagtgtcact acagcaggga tcaaagcggc
     6481 aaatgatttt gttactgcag gcggaactcc atcaggcatt ttgatctgcc agccgcgttt
     6541 cgtgattctg cagtagattt ctccagccaa gaaagcagcg atcattccaa tgaacattcc
     6601 ttttgcgcca agacgatcaa gtgaaagcac accagtcaca tcttcaccgc cagcggtttt
     6661 cattgcaaaa ggcgtaagaa tcaagaagct ggcaaaagac acggcaccac cgaaaatacc
     6721 ttcaacgtca taagattttg ataaataata gccgatacca aaagtaacaa aaaccgacat
     6781 gatgctcatt gttgcatttt gcccattacc gaataaattc gataaagttc cctttgttgc
     6841 gtcactgaaa aaaggcaagt tattgataac gacaacgatc gaaccgaaca tcgttaaagg
     6901 gaaggaaagc atgaaagcat cccgcagcac tgtaaggtaa cgattttgcc ctaataaatt
     6961 agctaagggc atgattttct cagaaagttt gtccataaaa ccgttcattt ttattcacct
     7021 caaatttatt tgcaatcgat tacatacaaa gtataacgcc aaaaatttta aatgtctagt
     7081 cattttcata aaaagtattg atttattttt tagggttcgt tatgattacg gtatcaagaa
     7141 aaaagagagg aagtaaaaaa atgaaaacct acacattccc agaaaatttt tggtggggtt
     7201 ctgctgctag cggaccgcaa acagaaggaa gagtaagtga tgatggtaaa ggagaaaata
     7261 tttgggatca ttggtatgaa ttagagcctg aaaaattctt cgatcaagta ggacctgaaa
     7321 aaacttcgca agtttacaca aaatacaaag aagatgttca gttaatgaaa gaaactggcc
     7381 ataacacatt tcgtacatct atccaatgga gtcatttgat tccagaaggg acaggtgaaa
     7441 tcaatcaaaa agctgtttct ttttatcgag attattttac tgaattgctg gataatggta
     7501 ttgaaccttt tgtgaacctt taccatttcg atatgccact accactgcaa gaaaaaggcg
     7561 gatggttatc aagagaaacg gtcgacgctt atgtttctta tgcagcagcg tgctttgaac
     7621 tgtttggaga tctggtcaaa aaatggttta ctcataatga accaatcgta cctgtagaag
     7681 gaggctactt gtatcaattc cactatccaa atcatatcaa tatgaaagaa gcggtccaag
     7741 taggctatca tgagaatcta gcaagcgcaa aggtaatcaa agcctaccat gaaggcggac
     7801 atgatggtga gatcggaatc atcttgaatc tgacaccaag ctatccgaga gatgaaacga
     7861 acccagaaga tgtaaaagcg gcgaagatcg ctgacgcatt cttcaaccga tcattccttg
     7921 accctgcagt gaaaggagag tttccacaag agttgatcga aattgtcaaa gaattggcta
     7981 taatgccaga gatccatgaa ggagacctag aactcatccg tgaaaacacg attgacctgc
     8041 taggaatcaa ttactatcag cctcgtcgaa tcaaagcaaa agaaactcca atcaatcacg
     8101 aacatagccc catgccagat gattattttg ataattacga catgcccaat aaaaaaatga
     8161 atccatatcg tggatgggaa atttacgaaa aaggaatcta tgatatttta acgaatacaa
     8221 gagaaaacta tggtaatatc aaatgctata tttctgaaaa tgggatgggg gtcgaaggag
     8281 aagaacgatt tgttaacagc gaaggaatta ttgaagatga ttaccggatc gagtttgtta
     8341 cggatcattt gaaatacgtc catcaagcaa tccaagaagg aaccaattgt gtgggttacc
     8401 acatgtggac atgtatggac aactggtctt ggacaaatgc ttataagaat cgctatggct
     8461 ttatcgctgt tgatttggca caagacggta aacggacgat caaaaaatca ggtcgctggt
     8521 ttaaaacggt ctctgaaaaa aacggatttg atgcctgata ttttttgata aaacgttcac
     8581 aaaatgtttg ttctaacact gtaatcgtgc tacactaaca gtgaaggagg cgtttacaat
     8641 ggtagatact tatcgtaata ttttagtagc aattgatggt tctgaaaagg cagaaaaagc
     8701 atttcttgag gcaatcacga ttgccaaacg aaatcaggca acgttgcaca tcctgtatgt
     8761 caatgaagta acaggcaatt actttggtga ttttgcattt gttacaacaa gccttcagga
     8821 agaattggat gaggttgcgg aaaaccagat gaaagaacat cgaaacttgg caattgaaaa
     8881 aggcctgaca gatatcgaga catatgttct ttacggttat ccaaagacac tgattgctaa
     8941 tttccatgaa tcgaaagaac caatcgatct gattgtcatg ggggcgacag gtctgaatgc
     9001 ggtagaacgt gcactagtag gttccactac ttcctatgta gtcaaccacg catcatgtaa
     9061 cgtcatggtt gtaaaataaa taaattaagg gaacagaaag caaaaaagca taacgcttct
     9121 tttgctttct gttcctttta tttttttgta aaaaattact ttacctgatg aagtaatcat
     9181 gctattctaa ttacaacgac agatggagta atatggagga aagaaaatga tttcaagtga
     9241 tggaatacgc ggatacaatg atgcgattat tttatcgatc cttaacaaaa acgattctta
     9301 tggatatgaa atttctaaaa cgattcgtga gaggacaaat gattcctatg tgatcaaaga
     9361 gacaacactt tactccgttt ttgcccgttt agaaaagaat caactgattg tttcgtatcc
     9421 tggagaagtg actcatggaa aaaagcggac atattataaa atcactgaaa tagggcgtaa
     9481 tcttttagaa gaaaaaaagc aagagtggct gacgacgaaa gacgtagtcg atcaattttt
     9541 ggagggaaat taaatggata caatccgtaa ttatttagac agtcttttta tcggagtacc
     9601 tcaaagtacc gagatagata agctgaaaac tgatttgttg gcaaatatgg aagaccatta
     9661 tcatgaattg atgggagaag gaaaaaatga acaggaggcc atcggtacag taatttcgac
     9721 atttggttca atcgatgaat tattagaaga actagatgtc gaaaaaaaac atcaagctga
     9781 cgagacggaa acaaacacag cttctattta tttaagcgaa gcagaaaact actggaaaga
     9841 atacagagcg gcaagtctcc aagtggcatc aggtgttcta ttcattagct tatcttttgc
     9901 aagttttctt ttcttttgtt cagcaggata tgtgttcatg ggtatttctt gtctgatttt
     9961 cggtatagca ttagctgtcg gtttttttat tgcttctggt atgaaaatca cacgtttaaa
    10021 tcactttttg caccatcgga aaatacctga aaaagtatta gcagaagcaa aagaaaaaga
    10081 agaagagtat cagcgaagtt ttggcttctc actgattgca ggaattggat tatgtatttt
    10141 ttcacttttc ccacttttgg cttctttaat gtggtatatg gatggtagta tcggtgcctc
    10201 tatatttttt gttacggtag gaacgggtgt cttcttgatc atttatggca gtctagtccg
    10261 tcattcttat cgtcaattca ctcaaagcgc ttattactgg tgaatgtatt cagatttgta
    10321 aaaatattct atttatccgg cgtagaaaaa aagactgtga cacgcttttg tcacagtctt
    10381 ttttatatat aataatttaa aaattcatca tctaaatccg tagaattttc tagattgctt
    10441 acccatgtaa aatcgttata gacagatttc agttgacttc tgcgccattg tacagggata
    10501 aaaagacgca tgctatcttc attgaatccg actaataatt tctttccgtc agtaatgatg
    10561 ggacgttgta acaaagtaaa atgcgagacg atcagatcga caagctctga ggtcgtacat
    10621 tggtcgattt ggtctttcaa atttttataa tcaatcgagc gtttagctaa tagatcgtca
    10681 aatccgtttt ctgttaatga caataaataa aatatctctt tccttgttaa aggctgttta
    10741 ggtgtgaaaa ttcgttctct gtaaggcatt tgatggtctt tcagccagtt tgccatcaga
    10801 tggtaactat gtgtgcgaga agaatagatc gtaagcataa aaaaactcct taaatagtcc
    10861 attaatttgt tagatctgtt gagataaata ataggtaagg agctgtgcat aagcattgat
    10921 caaaacaacc caaacatcat tagaaataac tttctaaagt cttaaacttg cgacgagaaa
    10981 caactagact aatagaaaaa atgtcttagc catttggctt cctgtttgtt tttttgctta
    11041 tgctgcagct cctcgggaaa gggttctaac aatttgaaga aggttgatga ttgatctagt
    11101 ttaaattgtg tgaaatttga aatctggtgt ttttactaga tgaatcacct aacaactatg
    11161 attataactt atagaacttc tgttttgcat tcggttttgt tatagttgtt ataaagtttt
    11221 attttattac a
//
LOCUS       DACDKM010000091        11060 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20834_2016
            SAMN12640370-rid9891663.denovo.091, whole genome shotgun sequence.
ACCESSION   DACDKM010000091 DACDKM010000000
VERSION     DACDKM010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640370
            Sequence Read Archive: SRR10040891
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11060)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11060)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742835.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742835.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:04:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,012
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 29 of 75
            Pseudo Genes (incomplete)         :: 33 of 75
            Pseudo Genes (internal stop)      :: 19 of 75
            Pseudo Genes (multiple problems)  :: 5 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11060
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640370-rid9891663.denovo.091"
                     /isolate="E20834_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(132..323)
                     /locus_tag="IV334_002172"
     CDS             complement(132..323)
                     /locus_tag="IV334_002172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8944227.1"
                     /translation="MEAGKVLQEVKALCADKKYEEAKSLIEKNKENLEEKYPIAQEFI
                     ELKQGTILHRFKHFFGITE"
     gene            complement(464..784)
                     /locus_tag="IV334_002173"
     CDS             complement(464..784)
                     /locus_tag="IV334_002173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8944228.1"
                     /translation="MVRPQEVRAPKEKIEILAIIEDGTQTKKGYSIALVKWKGKKGVA
                     LRWDGDNQQDKGFPVTANGYHPAWFVLPDKFMELYSYDYARAAASIKALDKLTEEQNE
                     MEEK"
     gene            complement(807..1241)
                     /locus_tag="IV334_002174"
     CDS             complement(807..1241)
                     /locus_tag="IV334_002174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HAP8944229.1"
                     /translation="MSKRYQNIMVAVDGSRQSIKAFAEALDLAKDNEAHLFIVSIINK
                     VELTHSAYAFSKIYADEKQRTEVAMLKKINDAKEYGIAEIHAIVETGDPRNLIANVIP
                     GQENIDLIVMGATGKGAIQQALVGSTASYVVSHAPCSVLVVK"
     gene            1576..2214
                     /locus_tag="IV334_002175"
     CDS             1576..2214
                     /locus_tag="IV334_002175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HAP8944230.1"
                     /translation="MTSLKTLLFGTTLAAGAAFFMGTTAHADEAYTVQSGDTLSTISQ
                     KYVGDNSLINAIAESNSISDINLIYSGQQLTIPTEGSAQAAAEPQAAVQEAPVQAEPV
                     QAEQPVVQETVQTETQAAPVAETQPAPAVTETAATPASTSSAKEWIAQKESSGSYTAT
                     NGRYIGRYQLDSSYLNGDYSAANQERVAEQYVTSRYGSWEAAKAFWEANGWY"
     gene            complement(2538..2621)
                     /locus_tag="IV334_002176"
     tRNA            complement(2538..2621)
                     /locus_tag="IV334_002176"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2585..2587),aa:Leu,seq:cag)
     gene            3149..3649
                     /locus_tag="IV334_002177"
     CDS             3149..3649
                     /locus_tag="IV334_002177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP8944231.1"
                     /translation="MKLIKLWEADLKKAYQLQTSFGPNESGFINNAYGYTFAEFLDYV
                     KKCEKTSLGIDLPESYVPATVFILESDEHEYVGIFNLRHKLNETLRNGAGHIGYGISP
                     NYRKKGYATQGLALVLQEAKKIGIDEALLSVNKKNLGSLKAQQKNGAAIYREDEKEYY
                     TKIKLM"
     gene            complement(3839..4183)
                     /locus_tag="IV334_002178"
     CDS             complement(3839..4183)
                     /locus_tag="IV334_002178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sulfur cluster assembly factor"
                     /protein_id="HAP8944232.1"
                     /translation="MSEANQERSGQEIELIKERILAALEMVIDPELGIDIVNLGLIYD
                     IEFNPENGETVIKMTLTTMGCPLADILTESIHDALKEVPEVTKAEVKLVWYPAWTTDK
                     MSRYARIALGIR"
     gene            complement(4337..5257)
                     /locus_tag="IV334_002179"
     CDS             complement(4337..5257)
                     /locus_tag="IV334_002179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HAP8944233.1"
                     /translation="MTAYHKNERLKKIVVILITGLTAAVALNYFLIPANVFSAGMNGI
                     AQIISSLLYEWFHIKIDTGTFIFLLNIPVFVLGFLKVGKRATILSFVNVVCVSVMTML
                     LPVGQITDNILMNALVGGVLLGLGVGLSLKMGFTTGGMDIISLVLSQTTGKTVGNYML
                     ILNGIIVVIAGFFFNWESALYTIISIYAMTQVIDAIHTSHQKVTAMIVTVKPEEVTEE
                     LAKRMVRGMTLLPSVGGYSKKQGNMIMMVITRYEMYDLDQIVHEIDDDAFINILPTHS
                     VFGRFANENEQKMYRTTGVLPEIKNRSQKK"
     gene            5444..5935
                     /locus_tag="IV334_002180"
     CDS             5444..5935
                     /locus_tag="IV334_002180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YajQ family cyclic di-GMP-binding protein"
                     /protein_id="HAP8944234.1"
                     /translation="MAKDASFDIVSEVNTEEVKNAIQQTTKELSNRFDFKDSTVEIKL
                     ENSNLVVLSDDDFKIEQIKDVLFGKLIKRNVPIKNIHFSESEHALGGKARQTADLVNG
                     IDRENAKKITTAIKNEKLKVKAQIQEDQIRVTGKNRDDLQAVIALLRKLDLPIELQFT
                     NYR"
     gene            complement(5959..8334)
                     /locus_tag="IV334_002181"
     CDS             complement(5959..8334)
                     /locus_tag="IV334_002181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HAP8944235.1"
                     /translation="MIKQSKISVRHLVEFILRRGSIDNRKKSNHTALEGARIHRKLQK
                     DAGENYEKEVFLKTTVQLDEYYLIVEGRADGIFKRDGIYYIDEIKTSEPRFEDLEPEQ
                     VELFFHQARVYAYIYSQEHDLQEINLQLTYFQTAEEVITRKVEHQTREQLETFFKKLT
                     EDYKEWLVFQENWRTVRNASLMALKFPHDEYRKGQRELAVAAYKTIRTKQKLFVEAPT
                     GTGKTISTLFPALKAVGEEEGEKIFYLTAKTITRQVAEDAMTALKDTGAEVKSVTLTA
                     KDKICFLDETTCNPDQCPYANGYYNRINEGLWDLLNHENQITREVIETYARKHTLCPF
                     ELSLDVSIWCDVIIGDYNYLFDPTVYLRRFFEDEKNEDYLFLIDEAHNLVNRSREMYS
                     AELSYEKTKRSKEAIPKEFKKLHRRFNKLLKEFDSIREIAKEDHWDYHHQKAPAESLV
                     KAGYQLSEKIKEWLAEFPEHPSQEQLLPYYFDLLHFLKVSEFYDDHYETTVEKTYRDL
                     IVKEFCIDPSLFLEQSLDKGKSSILFSASFSPLSYYQETLGGQKSLAYKLPSPFPEGN
                     QQVFIANYLETTYRKREHSLAKLVETIHAFADGKVGNYFVFFPSYQYLDLAVEAFRQQ
                     YPDSNVLIQETDMNEEERERFLTKFQTNPAETLIGFCVLGGIFSEGIDLRGDRLIGSM
                     IVGVGLPQMNHEQELIKSYYDEKEHLGFAYAYQLPGMNKVLQAGGRVIRGMADQGIVV
                     LADQRFSSFSYRRLFPQHWQTAREVRSVGELEEGIQRFWLSKEAVNQKNEE"
     gene            8660..9286
                     /locus_tag="IV334_002182"
     CDS             8660..9286
                     /locus_tag="IV334_002182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HAP8944236.1"
                     /translation="MHIERLKTGMIEENCYLVYNEEALLIIDPGEDAERIKQEIKKTN
                     QQPVAILLTHTHYDHIGAVEPLRAYYQIPVYVSPLEQKWLGDPILNLSGLGRHDDIAD
                     VIVSPAEYEFEMKTYTLGNMSFRVVPTPGHSIGSVSFIFDDFVVSGDALFRGSIGRTD
                     LYTGNLEQLLHSIRTQLFVLPDEFAVYPGHGDATTIEQEKRTNPFFNR"
     gene            9309..10013
                     /locus_tag="IV334_002183"
     CDS             9309..10013
                     /locus_tag="IV334_002183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HAP8944237.1"
                     /translation="MTETTLYIVRHGKTMFNTIERVQGWCDTPLTKQGQEGIHYLGRG
                     LKNVDFSFAYSSDSGRAIETARIILGEHSKGKEIPYFIEKRIREWCFGSLEGGYDMEM
                     WGVIPRVLNFKTYDEMFTTEVSFEQIANAVYNLDTAGWAETYETLRDRVWTGFEDIAH
                     HCEKNGGGNVLVVSHGLTIAFLLNLIDPKQPVRAGLLNGSVTKVTYANGRFSIHGIND
                     TSYIDNGKTHSRDSFL"
     gene            10205..11038
                     /locus_tag="IV334_002184"
     CDS             10205..11038
                     /locus_tag="IV334_002184"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HAP8944238.1"
                     /translation="MFFANIIKAVILGIIEGITEWLPISSTGHLILADEFIKLDASSQ
                     FMSMFNVVIQLGAILAVVVLYFHKLNPFSPSKSTVEKKDTWTLWFKVIVACIPAIIIG
                     LPLDDWLEANFHKFLPVAIVLIVYGIAFIIVEKRNKNKTPRWTSLNDFTYQAALLVGL
                     FQVLALVPGTSRSGATILGSILIGASRFVATEFSFFLGIPVMFGASGLKIVKYLAEGN
                     SFKMEETVVLLVGTFVSFVVSVLAIKFLLSYLKKNDFTAFGWYRIILGIILIGYWFIA
                     M"
BASE COUNT         3293 a         2162 c         1916 g         3689 t
ORIGIN      
        1 gacgctttca gtaatttttc ttattatttg gatctatttt ttatataaga atcagaattg
       61 aatccctcgc atgtttaaaa agaaaacgat taaagatgca gcaaatttct gccgatcttt
      121 aatcgttttt tctattctgt tataccaaaa aaatgtttga atcggtgaag gatcgttcct
      181 tgttttaatt ctataaattc ttgtgcgatc gggtactttt cttccaaatt ttctttgttc
      241 ttttcgatca ggcttttcgc ttcttcatat tttttatctg cacatagtgc ttttacttcc
      301 tgtaatactt ttcctgcttc catttgcctc accctcttgc cattttattg ctttaagttt
      361 tatttaagtt atacgcatta ttctagtttc atcccaaata accttttttc atttttactc
      421 ctcgaactaa tcagttttct aaaggctgat tggttcgtct tttttatttt tcctccattt
      481 cattctgctc ttctgttaat ttatctaatg ctttgattga tgctgcggct cttgcataat
      541 catagctgta taattccata aatttatcag gcagaacaaa ccacgctgga tgataaccgt
      601 tagctgtgac agggaatcct ttatcttgtt ggttatctcc atcccatctc agagcaacac
      661 ctttttttcc tttccacttt actaaagcga tcgagtatcc cttctttgtt tgtgtgccgt
      721 cttcaataat tgctaaaatt tctatttttt cttttggcgc acggacttct tgaggacgaa
      781 ccattttcta ttcctccctt atctcattac ttcacgacca acacactgca aggggcatgt
      841 gatacgacat aagaagcggt cgatccaact aatgcttgtt gtatagcacc ttttcctgtc
      901 gctcccataa cgatcaaatc aatattttct tgtccaggga tgacattggc aatcaaattt
      961 cttggatctc cagtttcaac tattgcgtgg atctcagcaa tgccgtactc tttggcatca
     1021 ttgatttttt tcagcatggc tacttctgtt ctttgttttt catctgcata gatttttgaa
     1081 aaggcatagg cactatgggt cagctcaact ttgttgatga ttgacacgat aaataagtgg
     1141 gcttcattgt cctttgctaa gtcaagggct tcagcgaacg ctttgatcga ttgtcttgat
     1201 ccgtcaactg ctaccatgat gttctggtat cttttactca ttggaaccgc ctcctttttc
     1261 ttttattgta acatctcaag caaaaataca aaaaatatac gcaatatgta aataaaatga
     1321 tgaacaaaag caatttcttt caagttattt atacatttct tttctcatca gaacacctgt
     1381 tctaatccca ttatttctgc attatgtgaa attcctcatc atttttcaca tcgagcgaac
     1441 atcattctgt tacaaaaaac gtggatttgt cataaattgt catgcttctt ttacctatga
     1501 aatattttca cgtgttaaag tattcctcgt tgaacacaac aaataaaact ttttaacgaa
     1561 taaaggagat ttttcatgac atcacttaaa actttacttt ttggaacaac tttggctgcc
     1621 ggcgctgcat tcttcatggg aacgactgct catgcggacg aagcttatac cgttcaatca
     1681 ggtgatactc tatcaacgat ttctcaaaaa tacgttggtg ataactcctt gatcaatgcc
     1741 attgcagaat caaattcaat ttctgatatt aacctgatct actcaggaca acaattaaca
     1801 atcccaacag aaggctctgc acaagcagct gctgaacctc aagcagccgt tcaagaagca
     1861 ccagtccaag ctgaacctgt tcaagctgaa caacctgttg tacaagaaac ggttcaaacg
     1921 gaaacacaag cagcgcctgt tgctgaaaca caacctgctc ctgctgtaac agaaactgct
     1981 gctactccag caagtacaag ctctgcaaaa gaatggatcg cacaaaaaga atcaagcggt
     2041 tcttacacag caacgaacgg acgttacatc ggccgttacc aattagattc ttcttactta
     2101 aatggtgact actctgctgc aaaccaagaa agagtagcag aacaatatgt tacttcacgt
     2161 tatggttctt gggaagcggc gaaagcattc tgggaagcaa acggttggta ctaagattca
     2221 ttatttgaac gtttaaacac attcaaaata tagtaagagg tcatgcgatt tattctttcg
     2281 catgacctct ttttgagttt aagtttagtc tttattcctt aactatcgtt tgccagtatt
     2341 tcttgtatca gaatctataa atctactatt tgaatcaatc tattaggttt taaactgtcc
     2401 caccttcaca caaatttttt ttgatttatt tgaatctggt tccgttgttt aaaatttttc
     2461 ctatattctt ttagataata tgttattttc tttctcatcg ctccaggtgt accttctctt
     2521 tataaaaaaa caaattatgc ggctaagaag actcgaactt ccacgaggtc accctcacta
     2581 gcgcctgaag ctagcgcgtc tgccaattcc gccatagccg catcacatct tttagtttag
     2641 gattacatgt gttttttgtc aagaacatat ttttgttctt tttcatttct gtacgccctc
     2701 ccttatgcca tctacacaaa taattcatcc gagcaactcc atcatctatt gagggttggt
     2761 gaaaaaatct gttttatcca tatgatcgat ttcaatgtta aacgaacgac tctgggatta
     2821 gtaactattt tatttttttc ttcatttctt ttttcctctt tttctcgatt cagctatctt
     2881 ctttcatttg gatattgatt atttcttctg ttgattacta tccttctgga attctagacg
     2941 ctgaagtaat tcacgttttc tttcttctag agatcataag taacgaatga acgaacttca
     3001 aataattttt ttattgtaat tcatatttgt attgttcgat gattcttaat acgatatgac
     3061 acaataaata aaaataggca tggctattta caacaatagt gctatagtag aaaacaaatg
     3121 aataaggtta cttaaagagg tgaatacgat gaaactgatc aaactatggg aagcagattt
     3181 aaagaaagct tatcagctac aaactagctt tggaccaaat gagagtggat ttatcaacaa
     3241 tgcttatggc tatacatttg ctgaattttt agattatgta aaaaaatgcg aaaaaacgtc
     3301 tttaggaatc gatcttcctg agagttacgt tcccgcaacc gtctttattt tagaaagtga
     3361 tgaacatgag tatgtaggaa tttttaattt gcgccataaa ctgaacgaaa cattaagaaa
     3421 cggcgctggc catattggtt acggcatctc ccctaattat cgtaaaaaag gatatgccac
     3481 acaaggatta gcattagtac tacaagaggc aaaaaaaatc ggcattgatg aggcactttt
     3541 gtctgtaaat aaaaaaaatc ttggcagtct taaagcacaa caaaaaaatg gggctgcgat
     3601 ttatcgtgaa gatgagaaag aatactatac gaaaatcaaa ctgatgtaaa aaagaaggat
     3661 tccaaaagga aagggatttg aatgtccttt atcaatagaa cacatttatt taattaactt
     3721 ataatatagc agacaaaatt ttataaacaa agaaaccttt tatctctttt cttctagaaa
     3781 ggataaaagg cttttttgtt tttcctattt gcttctcata gctagtcagc caagatttct
     3841 atcgtatccc caacgcaatc cgtgcataac ggctcatctt atctgtcgtc catgctggat
     3901 accagactag tttgacttct gctttcgtta cttctggcac ttcttttaat gcgtcatgga
     3961 tagattctgt taaaatatct gctaatggac agcccatagt tgttagagtc atcttaatga
     4021 ctgtttctcc attttcagga ttgaattcta tatcataaat caagcctaaa ttgacgatgt
     4081 cgattcctaa ttcaggatca atcaccattt ccaaggcagc aagaatacgt tctttgatta
     4141 gttctatttc ttgtccagac cgttcttggt ttgcttcgct catcgtacga cactccttaa
     4201 cacgattttt atttatcata ctcttttttt agctgcttgt aaatgagaat ttagaagtaa
     4261 gattctcgtt taaatttata aaaatgttct tattagatgt aatagattaa atttattcta
     4321 aaataaacct gattcctcat tttttttgtg aacgattttt tatttctggc aatacgccag
     4381 ttgttcgata cattttttgt tcgttttcat ttgcaaaccg gccaaatact gaatgagttg
     4441 gtaaaatatt gataaatgca tcgtcgtcga tttcatgaac gatctgatca agatcataca
     4501 tttcgtagcg ggtgatgacc atcatgatca tgttcccttg tttttttgaa tagccaccga
     4561 cagacggcag taatgtcatt ccacgtacca tccgttttgc tagttcttct gttacctctt
     4621 ctggcttgac ggtaacgatc attgctgtta ctttttgatg actcgtgtgg atggcatcaa
     4681 ttacttgagt cattgcataa atggaaataa tcgtatacaa ggcactttcc cagttaaaga
     4741 agaagcctgc gatgactaca atgataccat ttagaatcag catgtagttt ccgactgttt
     4801 ttccagttgt ttgtgaaagt acaagagaga tgatatccat cccaccagtt gtaaagccca
     4861 tttttaaaga taatcctacg ccaagaccta ataaaacacc gcctactagg gcattcatca
     4921 aaatattatc agtgatctgt ccgactggaa gcagcatcgt catcaccgat acacatacaa
     4981 cattcacaaa actaaggatc gttgctcgtt ttcctacttt gagaaaccct aatacaaaca
     5041 cagggatatt caaaagaaaa ataaatgttc ctgtgtctat cttgatatga aaccattcat
     5101 ataaaagcga ggaaatgatt tgagcgatcc cattcatccc tgcagaaaag acattggctg
     5161 gaatcaaaaa ataatttaaa gcgaccgcag cggtcaatcc ggttatcaaa ataaccacta
     5221 ttttctttaa tctttcattt ttatgatacg cagtcataat tttactcaca agcacctctc
     5281 ccctactttg gtattccaaa tgtaagaata acagtttatc tattaaaata aaatacttcg
     5341 aactcaaatg agaagaattt tagcaaacag gtaagaatct gccctaatcc tatgataaga
     5401 tagatgtatg gtaaatattt atatgtatat caggagggtt cctatggcaa aagatgctag
     5461 cttcgacatt gtatcagaag tcaatacaga agaagtaaaa aatgcgattc agcaaacaac
     5521 aaaagaattg tccaatcgct ttgacttcaa ggattctaca gtagaaatca aattagagaa
     5581 cagcaatcta gttgtattga gcgatgatga tttcaaaatc gaacagatca aagatgtatt
     5641 atttggtaag ctgatcaaaa gaaatgtgcc aatcaaaaat atccattttt ccgaatcaga
     5701 acacgctcta ggtggcaaag ctcgacaaac tgccgatttg gtcaacggaa tcgatcgtga
     5761 aaacgcgaag aaaatcacta cagctatcaa aaatgaaaaa ctgaaagtaa aagcacaaat
     5821 ccaagaagat cagattagag tgacaggtaa gaaccgtgat gatctacaag ctgtgattgc
     5881 tttattacgt aaacttgact taccaatcga gttgcaattc actaactatc gttaatcaaa
     5941 aaacgaggct gacgtctgtc attcctcgtt tttttgattt actgcttctt ttgataacca
     6001 aaaacgctgt attccttctt ccaattcccc aacacttcgt acttctcttg ctgtttgcca
     6061 atgttgtggg aataaacgtc gataagaaaa actagaaaat cgttgatctg ccaatacaac
     6121 gattccttga tccgccatgc cacgaatcac tcgaccacca gcttgcaaga ctttgttcat
     6181 tccaggaagc tgataagcgt aagcaaatcc aaggtgttcc ttctcatcat aatatgattt
     6241 gatcagttcc tgctcatgat tcatctgcgg caaacctaca cctacgatca tactgccgat
     6301 caatcggtcc cctctcaaat cgattccttc tgaaaaaatc ccgccaagta cgcaaaatcc
     6361 aatcaatgtt tcagcgggat ttgtttgaaa ttttgttaaa aatcgttcgc gttcttcctc
     6421 attcatgtcc gtttcttgga ttagcacatt gctgtcggga tattgctgtc tgaatgcttc
     6481 caccgcaaga tctaaatatt gataagaagg aaagaaaacg aaataattcc cgacctttcc
     6541 atcagcaaaa gcatgaatcg tctctactaa ttttgccaga ctgtgttccc tttttcgata
     6601 agtcgtctct aggtaatttg cgatgaaaac ttgctggttt ccttctggga aaggactcgg
     6661 cagtttgtaa gccagacttt tttgtccccc cagtgtttcc tgataataag agagtggtga
     6721 aaaactagca gaaaaaagaa tactgctttt tcctttatcc aacgactgct caagaaacaa
     6781 gcttggatca atacaaaact cttttactat cagatcgcga tacgtttttt cgactgttgt
     6841 ttcgtagtga tcatcataaa attcactgac cttcaaaaaa tgaagcaaat caaaataata
     6901 aggaagcaat tgttcttgtg atgggtgctc aggaaattct gccagccatt ctttaatttt
     6961 ttcgcttaat tgataacctg cctttactaa agattcagcc ggtgcttttt gatgatgata
     7021 atcccaatga tcttcttttg ctatttctcg gatcgaatca aattctttta atagtttgtt
     7081 gaaacggcga tgaagttttt tgaattcctt tggtatagct tccttacttc gtttcgtttt
     7141 ttcataagat agttctgccg aatacatctc tcgggaacga ttcaccaaat tatgtgcttc
     7201 gtcaatcaaa aacagatagt cctcgttttt ttcatcttca aaaaaacgtc ttaaataaac
     7261 agttggatca aataaataat tgtaatcccc aatgattacg tcacaccaga tactgacatc
     7321 taatgatagt tcgaaaggac aaagtgtatg ctttcttgca taagtttcga tgacttcacg
     7381 agtgatctga ttctcatgat tcaaaagatc ccataaacct tcattaatcc gattgtaata
     7441 accattggca tatgggcact gatctggatt acaggtcgtc tcatctaaaa agcagatctt
     7501 gtctttcgcc gtcaaagtga cactttttac ttcagctcct gtatctttta atgctgtcat
     7561 agcatcttca gctacttgtc tagttattgt tttagccgtt agataaaaga tcttttctcc
     7621 ctcttcttcc cctacagctt tcaaagcggg gaataacgta gagattgttt ttcctgttcc
     7681 ggtaggtgct tctacaaaca atttctgttt cgtacggatc gttttataag cagctaccgc
     7741 tagttcacgc tgacctttcc tatactcatc atgggggaat ttcaaagcca tcaaagaggc
     7801 attgcgtaca gtgcgccagt tttcttgaaa aacaagccat tctttataat cttcagtcag
     7861 ctttttaaaa aaagtttcta actgctctct tgtctgatgt tcaacctttc tcgtgatcac
     7921 ttcttctgcc gtctggaagt aagtcaattg cagatttatt tcttgtaaat catgttcttg
     7981 actatagata taggcataca cccttgcttg atgaaagaaa agttccactt gttctggttc
     8041 taaatcttca aatcgtggtt cggaggtttt gatttcatct atataataaa taccgtctct
     8101 tttgaaaata ccgtctgcac ggccttcaac gatcaggtaa tattcatcta attgaactgt
     8161 tgtttttaaa aagacttctt tttcatagtt ttctcccgca tctttttgga gcttgcggtg
     8221 gattctcgct ccttctaatg cagtatggtt acttttttta cgattgtcga tacttccacg
     8281 tcgcaaaata aattctacta agtgccgcac agatattttt gactgtttga tcatttccac
     8341 cacctctttc tctctaagat tacctgaaaa aacctagttt agcaaaaaga gctctctttc
     8401 caaacataaa aaaacaccct gctgattagg cagggatata ggatatagaa tttggatttt
     8461 tggatgaatc taatgtaaca aggacatatt aaaatgctat gaaaacttac tttccttctc
     8521 tttagaatat ccaaatactt ttcatatagt ctgataattc agataagtaa agagaaataa
     8581 gtacctgttt ttgtttccta gcaatgaatg cgttatacta atggatgaaa gaagttttgg
     8641 ctatcaagga ggaatgcaca tgcatatcga aagattaaaa acaggaatga ttgaagaaaa
     8701 ctgttatttg gtctacaacg aagaagcact tttgatcatt gaccctggag aagatgcgga
     8761 acgaatcaaa caagaaatca aaaaaacaaa ccaacagccg gtagcgattt tgcttaccca
     8821 tactcactac gatcatatcg gtgcagtgga acctttacgc gcgtattatc aaatcccagt
     8881 ttatgtcagc ccactagagc aaaaatggct aggtgatccg attttgaatc tttctggact
     8941 cggtagacat gatgatattg ctgatgttat tgtctcgcct gcagaatatg aatttgaaat
     9001 gaaaacttat actttaggaa acatgtcttt tcgcgtcgtc cctaccccag ggcattctat
     9061 aggaagcgtc agctttattt ttgatgattt tgtcgtttct ggcgacgccc tgtttagagg
     9121 aagtatcggt agaaccgatc tgtatacagg gaatttagaa caattacttc atagtattcg
     9181 gacccagctg tttgtattac ctgatgaatt tgcagtctat ccaggacatg gcgatgcaac
     9241 gacgattgaa caggaaaaaa ggacaaatcc ttttttcaat cgttaaaacg acttaggagg
     9301 agaataaaat gacagaaact acactttata tcgtacgaca cggaaaaacg atgttcaata
     9361 caattgaacg agtacaaggc tggtgtgata cacccctcac aaagcaagga caagaaggca
     9421 ttcattattt aggtcgcggg ttaaaaaatg tcgacttttc atttgcttat tctagtgaca
     9481 gtggtcgtgc aatcgaaact gcccgtatta ttctaggaga acattctaag ggaaaagaaa
     9541 ttccttattt tattgaaaaa aggattagag aatggtgttt cgggtctctt gaaggtggat
     9601 atgacatgga aatgtggggt gtgatccctc gtgtattgaa ttttaaaaca tatgatgaaa
     9661 tgtttaccac agaagtttct tttgaacaaa ttgctaatgc tgtatacaac ttagataccg
     9721 ctgggtgggc ggaaacttat gaaacattgc gagaccgtgt atggacagga tttgaagata
     9781 tcgcccatca ctgcgaaaaa aatggtggtg gaaatgtttt agtcgtatct catggtttga
     9841 cgatcgcctt tttgcttaat ctgatcgatc ctaaacagcc tgttcgtgct gggcttttaa
     9901 atggcagcgt aacaaaggtc acttatgcaa acggtcgatt ttctatccac ggcatcaatg
     9961 atacgtctta tatcgataac gggaaaacac atagtcgcga ctcgtttcta taatggggat
    10021 attttaattt ttaccagaga atcataaaag gcaaagtctg aacgagggct ttgtcttctt
    10081 ttttctcttc gtgtacttat ctgctttatt tctcaagttt ttcttaatat taaaaaacag
    10141 attgggtttc tcaaaaaata acggtatact ataagatgta tcttaattgg gaaggaagga
    10201 atttatgttt tttgcaaata ttatcaaagc agtgattctc ggtattattg aaggaataac
    10261 ggaatggctg cccatcagta gtactggtca tttgatccta gcagatgaat tcatcaaact
    10321 ggatgccagt tcacaattta tgtcgatgtt caatgtcgtt atccaattag gggcgatctt
    10381 ggctgtcgtc gttctttatt ttcacaagtt aaacccattt tctccaagta aatcaacggt
    10441 agaaaaaaaa gatacgtgga cactttggtt taaagtgatc gttgcttgca taccagccat
    10501 catcattggt ttgcctttgg atgattggtt agaagcaaat ttccacaagt tcttaccagt
    10561 tgccattgtt ttgatcgttt acggtattgc ctttattatt gtcgaaaaac gcaacaaaaa
    10621 caaaacacct cgttggactt ctctgaatga ctttacgtac caagcagctc ttcttgttgg
    10681 gttattccaa gtcttggcac ttgttcccgg aacatcccgt tctggcgcaa cgatcctagg
    10741 atctatcttg attggtgctt cgcgttttgt cgctacagag ttttcatttt tcttagggat
    10801 tcctgtcatg tttggcgcaa gcggcttgaa gatcgtcaaa tatttagctg aagggaattc
    10861 attcaaaatg gaagaaactg ttgttcttct tgtaggaaca ttcgtatctt ttgtggtttc
    10921 cgtcttagct atcaaattct tactaagcta tttgaagaaa aacgacttta ccgcttttgg
    10981 ctggtatcgt attatccttg gtatcatttt gattggttat tggtttattg caatgtagaa
    11041 aaaaccagaa cacttctctt
//
LOCUS       DACDKM010000092        11052 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20834_2016
            SAMN12640370-rid9891663.denovo.092, whole genome shotgun sequence.
ACCESSION   DACDKM010000092 DACDKM010000000
VERSION     DACDKM010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640370
            Sequence Read Archive: SRR10040891
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11052)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11052)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742835.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742835.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:04:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,012
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 29 of 75
            Pseudo Genes (incomplete)         :: 33 of 75
            Pseudo Genes (internal stop)      :: 19 of 75
            Pseudo Genes (multiple problems)  :: 5 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11052
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640370-rid9891663.denovo.092"
                     /isolate="E20834_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            949..1422
                     /locus_tag="IV334_002185"
     CDS             949..1422
                     /locus_tag="IV334_002185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2975 domain-containing protein"
                     /protein_id="HAP8944239.1"
                     /translation="MKFKTLLLKFVVLGISAVFILFGVLFYTQIATSEKGFNFDWQTT
                     LFVSVIYFALLLGIVIAYFLLKLLKKIEDNQAFSTQSLNILKKIERTILFIGFAFFGL
                     LPKFMKVADLDDAPGLMLVGIAVVFIPFAVYTLTSVLEKLLEHAILLKTDHDLTV"
     gene            1431..1658
                     /locus_tag="IV334_002186"
     CDS             1431..1658
                     /locus_tag="IV334_002186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP8944240.1"
                     /translation="MAIIVNLDVVLAQKKMSVTELSEKVGITMANISILKNGKAKAIR
                     FSTLEKICQVLECQPGDLLTYKNDSNTIPEE"
     gene            2294..2665
                     /locus_tag="IV334_002187"
     CDS             2294..2665
                     /locus_tag="IV334_002187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8944241.1"
                     /translation="MPFIVYFFLSLLVIYIPLPTFFLLVNRLAEQHDTIAALTKLCLL
                     LFVCLDYFTSYYWLKQTRVKKRYYFFLVFFNLFEFLIHCFLLHQYHTIHLWIIWVFKV
                     VVLFCMFTFPISRKFREALFY"
     gene            complement(2921..3163)
                     /locus_tag="IV334_002188"
     CDS             complement(2921..3163)
                     /locus_tag="IV334_002188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HAP8944242.1"
                     /translation="MGFIWSLIVGGIIGAIAGAITNKGSSMGIIANVVAGLVGSALGQ
                     ALLGSWGPSIAGMAIVPSLIGAIILVLIVSFFARKL"
     gene            complement(3195..4097)
                     /locus_tag="IV334_002189"
     CDS             complement(3195..4097)
                     /locus_tag="IV334_002189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HAP8944243.1"
                     /translation="MNNNNKNNNNKMNNQKPVSAHPDPTTPNQSNGVNDEKNKTAPVS
                     AHPDPVVPNVQQGVEEKKHDAEKHAPHHPGTPESKVPEQHDAIDPSKPVEEQKHPSDH
                     AKAQEKLSSDHTNGQGKLGSDHTNNQGKHDSEQEDSMRGELNYEDKVVQKIIGIAIEQ
                     VDGLLSANGGFFSNVAGKLVNTDNVTAGIETEVGKKQVAVDMDVIVEYGKDIEKIFEE
                     MQEVIGKEVKNMTHLEVIEVNANVVDIKTKEEFEKDQETVQDKVTDAAKKTGEFASNQ
                     TDKVKSAAGSGAQKVKENTEPRVQ"
     gene            complement(4110..4298)
                     /locus_tag="IV334_002190"
     CDS             complement(4110..4298)
                     /locus_tag="IV334_002190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2273 domain-containing protein"
                     /protein_id="HAP8944244.1"
                     /translation="MKELFSTYKLPIIFGVLGLILAVLWITVGFFKTLLILIMAAIGV
                     AIGFYLKQTKILDHYFKS"
     gene            complement(4312..4875)
                     /gene="amaP"
                     /locus_tag="IV334_002191"
     CDS             complement(4312..4875)
                     /gene="amaP"
                     /locus_tag="IV334_002191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline shock response membrane anchor protein
                     AmaP"
                     /protein_id="HAP8944245.1"
                     /translation="MNKGVKTIGVIGTLLLLSVLLFAILLSATYPLPFQLEKFRFFSI
                     TNSYVQQYVFWLAVAFAILTIITLLVFLFYPKTIGNFLLKHGDGELTLDKKAIEGMVR
                     SHLHEDEFIHSPKVNVKATKNKIAVNVKGELKRTSALIGKTGTLMKEIENEVQHILGT
                     KEPVKVSVKYTGFEEPAKRSNDRKRVE"
     gene            complement(4913..5806)
                     /locus_tag="IV334_002192"
     CDS             complement(4913..5806)
                     /locus_tag="IV334_002192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAP8944246.1"
                     /translation="MTEPKLIDPRNLYHTEKFPKQDQDTPALQQNMIPVPDCGEESYE
                     GNNQLENRRVLITGGDSGIGRAAAIAFAREGADIALQFFPGEEKDAEEVAKYVKVAGR
                     KIVLLPFDLRDEQAPKEIIDKAVSELGGLDTMVLNAGQQISCQSIAELPLEQVKDTFM
                     INIIAMFALVKEAVPHIPAGGAIVTTTSVQAFNPSEHLLDYAATKASIANFTIGLAKQ
                     LAPKGIRVNGVAPGPIWTPLQLDEGQPSDSIPEFGQQSLLERAGQPGELAPVYVFLAS
                     NKASYVTAQIYGVTGGEAINL"
     gene            complement(5884..6822)
                     /locus_tag="IV334_002193"
     CDS             complement(5884..6822)
                     /locus_tag="IV334_002193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HAP8944247.1"
                     /translation="MRYYFVDEEKNVIETDEKKYNWLVLDSAKPEEIEQAIEEFELPD
                     DIFVGTEYPEEVSRMERLYETKLEHPFSLVVINLDQSDNRIEKKLTPISFVLSDNLLI
                     TCIDRKTEFVDRLLTKHGNHLNSFEKIITYTLLDIYTHYVKELREMKKRIDALDKAAR
                     KTTENEELYKQADLERDIVYIDHTLRDQRETMDQLWNSETFIKRLDDEQLLYDVHLRQ
                     RQTEKMIEIYRDLLESIGDLFNGMMDNNLNHIMKYLDSAALIISVPALFAGIWGMNTG
                     GLPGKSSALGFILVVFGSLIAAVVLGYHLYRKDYTN"
     gene            complement(6856..7206)
                     /locus_tag="IV334_002194"
     CDS             complement(6856..7206)
                     /locus_tag="IV334_002194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HAP8944248.1"
                     /translation="MEITMSKNAVETLIEKVGENTKIALALINDSDPFLRDKGAFAKG
                     SFFQIIPFVSEFGEYATKIEHPLLDIYTSKLEQNYFGKRLNMDFNKQLDSFSLENEIA
                     VLDYNIKLKNCFFS"
     gene            complement(7239..8141)
                     /locus_tag="IV334_002195"
     CDS             complement(7239..8141)
                     /locus_tag="IV334_002195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HAP8944249.1"
                     /translation="MNKKEIAVVASCNTKFVPHLAALFVSVLDNCNPSKFVRFYVIDD
                     DIDFESKQLLRFSVKNARMNSDVEFLKINKEFFTNVVISDRIPETAYYRIAIPELFRG
                     TEVERILYMDCDMIALQDISKLWRLDFGDSIVAAVEDAGFHQRLEKMKISAKSMRYFN
                     SGLMLINVKKWLDENITQKVLDFIEHNPEKLRFHDQDALNAILHDRWLPLHPRWNAQG
                     YIMAKAKKHPTAAGEREYEETRNNPYIIHFSGHVKPWSKDFEGPTKKYYEKYAGMTAF
                     RCVAKFPKYPKYAKVQPKTDVVVK"
     gene            complement(8134..8991)
                     /locus_tag="IV334_002196"
     CDS             complement(8134..8991)
                     /locus_tag="IV334_002196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HAP8944250.1"
                     /translation="MEKRYGVVPVVTASDENYAPYLSVMIATALENCNKTRRIKFYVI
                     DDGLSEYSKEGLEETVNKYSSNASIQFLTVEKDIYEDFLVSDHITTTAYLRISLPNLL
                     AKEDYKKVLYLDSDVLVLDDIVKLYDEPLNGKTIGAIIDPGQVKALERLGIDSDDLYF
                     NSGVMVIDIDQWNKKEITEKTIHYLSENGDRIIYHDQDALNAVLYEDWEQLHPKWNMQ
                     TSLIFERHPAPNEKYERQYKEGNEKPSIVHFTGHDKPWNTLKDHPYTNLYLKKLAHST
                     LTKVGEINE"
     gene            9325..10134
                     /locus_tag="IV334_002197"
     CDS             9325..10134
                     /locus_tag="IV334_002197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAP8944251.1"
                     /translation="MKLAAIDLDGTLLDRNGKIPEENIQALRKFDENGGIVTVATGRN
                     SISAKDIFSELGIGGYLISSNGSLIAEMKDGKMAKVLRRSKIDSDTLRKAFFYAQEAK
                     ISIIASRETQDDQLTFDESNLVKDDPYYEHFNLQNHSFDQILNMLEDPSLSYLKLALT
                     DKSEEKLQKLQRDLQADGIQSVFSDPHFLEITPTGITKAHSLVFLADYLGIESSDIMA
                     FGDQENDIAMLNYSGLSIAMGNAQRQVKEIANQVTLTNEEAGVAYILKNFY"
     gene            10174..10671
                     /locus_tag="IV334_002198"
     CDS             10174..10671
                     /locus_tag="IV334_002198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HAP8944252.1"
                     /translation="MLVIIDMQNHILDPNSEFYIPESDQLAERIAQRLAKARQNNEYI
                     LFTRDIPVELKDKEEEREDLQLIPLLSPQENERVIKKYYFTIPPETLAEIQETLFESK
                     EEQKEIEVVGIETNLCVLSNLIGLQSAFPEADFFVDPMLVSSRKHGESALELLKDFNV
                     SIVES"
BASE COUNT         3235 a         2066 c         1854 g         3897 t
ORIGIN      
        1 ttttcggaga attaaaaaat acaataaata actttagtta ctttaatgcg ttcttacttt
       61 atttttatat tatcattttt cttattcact tctttggttt atactattct tataaataaa
      121 aacgaggtat gtcctatgat gaaaagattg tactattcat tgatcataac gattggctat
      181 ttgattgtat caaatctagg aaatatggta tttggtatca gtaaagaatt cagttggact
      241 actacattat gggaatctct attctttttc atcttcgtat ttttgttaca aaattatcga
      301 aaaaaataaa catgtgccat ctatctgttg gcacatgttt taggatttat ggtacttttt
      361 attcggcaat ccattttatt tttctacttc ttcttaatac ctctaacact tttgatttat
      421 aaactaatcc aatgcatcta cttgcgctta ttaagctatt tcagctagat agtaccaccc
      481 catgcgtcaa tgcttgattt tccttttgta taacattgta taagcaataa aggacaaata
      541 agtggtgatt acaccaaacc attgaataag aaaaattctg atacgtaaat aaacatgaac
      601 ttgaaaaaat taatgaatcg gttagctact acgtagttgt cttttttctc atacagttct
      661 gttaacttac acttctcttc gctagataat aaaaaatcct ccaactgtat tgtccaatat
      721 aatcccaggc atctaaacta gcattataga agagacacac atagaatcta aaataactgg
      781 ttttaaatgt ttcttacaat caaaaaaagc tgttaaatgc actccacacc ttcatactct
      841 atcatttttt ataaacgata tatttttatt gtttttcgat aaaaaatagt tgttttacaa
      901 taattattct gctatatttc agctatcaaa gataaaagag ggataaaaat gaaatttaaa
      961 acattgctat taaaatttgt cgttttaggt ataagtgccg tatttatttt gttcggcgta
     1021 cttttttata ctcagatcgc tacaagcgaa aaaggattca attttgattg gcagaccaca
     1081 ttatttgttt ctgtcattta ctttgccctt ctcctaggga tcgttattgc ttatttctta
     1141 ttgaaactat tgaaaaaaat cgaagataac caagcttttt ccactcaaag cttaaacata
     1201 ctaaaaaaaa tcgaacgaac cattttgttt attggtttcg ccttttttgg cttacttcct
     1261 aaatttatga aagtagcgga tttagacgat gctccaggtt tgatgttggt aggaatcgct
     1321 gtcgtattca ttccttttgc agtatacaca ttaacaagcg tgctggaaaa actgcttgaa
     1381 catgcgattt tacttaagac agatcatgat ttgacagtgt gaggtgcgac atggcaatta
     1441 tcgttaattt agatgtagta ttagctcaaa aaaaaatgag tgtgactgaa ttgagtgaga
     1501 aagtgggcat tacgatggca aacatctcta ttctaaaaaa tggaaaagca aaagccatcc
     1561 gtttttccac tctcgaaaaa atctgccaag ttttggagtg tcaaccaggt gatttgttga
     1621 cctataaaaa tgactcgaat actataccag aagaataaaa taaagtagaa caaacagtaa
     1681 ataaaaaata atctgaatga tagatgtgtg agacttttag aagttattta cgtctattat
     1741 tcagattttt tatttcgatt gaataccgtt ttatctataa ataagatatg tactgactca
     1801 tacaaagggg gccttttctt ctccatgtac tatcactttt cctgatacaa ctggtttcct
     1861 gttacgttgt agttggatct gcttttagta taatacttta ttcattccac cagactgata
     1921 gcagtttgag cagtctaaat aatcatgtag tattacaggc cattctgttt cataccttga
     1981 agtgcttaaa acttcagcta atggcccatt gtgttaactc ttactccatt cttttttagt
     2041 aagctcccat atatactgta aagccttcta tatattatgt catgttgctt tttaacattt
     2101 actatgtagt tttgataaaa tgatttgaat aacagtgatg gacaattctt ttttattttt
     2161 aacattattt tgataataat gattatttat ttaaaaatac attagataac atttgttacg
     2221 acacgaaaaa gcttcacaaa caaatatcgt tattttaaaa tgagggcgaa ctcacacaat
     2281 taggaggaat agtatgccat ttattgttta cttttttctt tcattactcg tgatctatat
     2341 tccattacca acatttttcc tgctcgtcaa tcgtctagca gagcaacatg atacgattgc
     2401 tgctcttacg aaactttgtt tacttctatt tgtttgtcta gattacttta cgagctatta
     2461 ttggctaaaa cagacaagag taaaaaagag atattatttc ttccttgtct ttttcaatct
     2521 ctttgagttt ctgatccatt gctttttact tcatcagtat catacgatcc atttgtggat
     2581 catctgggtt ttcaaagtgg tcgtattatt ttgtatgttc acctttccta tctcaagaaa
     2641 atttagggag gcattgtttt attaaagaag cttctctgta tcaataaaga cacatttaag
     2701 gaaaaacttt taaaaatcta attgggaatt tgcccgcata aataagaggc tgtgacaaaa
     2761 gtcttgtcac agcctcttat tccgtataaa cagtgttcaa aagcttgcat cttcgataat
     2821 atgcccaacc aaatgaaaaa tatgagaagc tatttttgtt tggctgttct tattacttga
     2881 tgaagaacag ctttttcacg acctcttttt aaaagtcttt ttacagtttt cgagcaaaga
     2941 atgatacgat tagtactaaa atgatggcac caatcaatga tggcacgatt gccattcctg
     3001 caatacttgg tccccaagaa cctaataatg cttgaccaag tgctgatcca actaaacctg
     3061 ctacgacatt tgcaataatc cccatcgaag aacctttatt cgtgattgct cctgcaattg
     3121 ctccgataat tcctccgacg ataagtgacc aaataaatcc catgtttcgt tcctcctaat
     3181 gttttacttc cttattactg aacccgtggt tctgtatttt cttttacttt ttgtgcgccg
     3241 cttccagctg ctgattttac tttatctgtt tgattcgaag caaattcacc ggtttttttc
     3301 gctgcgtcag ttactttgtc ttgcaccgtt tcttgatctt tttcaaattc ttcttttgtt
     3361 ttgatgtcca ctacgttagc gttgacttcg atcacttcta aatgtgtcat attttttact
     3421 tctttgccga ttacttcttg catttcttca aagatttttt cgatatcttt gccatattcc
     3481 acaatgacat ccatatcaac tgctacttgt tttttaccga cttctgtttc gatcccagcc
     3541 gttacattat ctgtattcac aagttttcct gccacattag agaagaaacc accgtttgca
     3601 gataataaac cgtctacttg ttcaattgcg attccgatga ttttttgcac aactttatct
     3661 tcataattca gctcaccgcg cattgaatct tcctgttcag aatcatgctt tccttggtta
     3721 tttgtatgat cagaaccaag ttttccttgt ccgtttgtgt ggtcagaact aagtttttct
     3781 tgtgctttcg catggtcaga aggatgtttt tgctcttcca caggtttaga aggatcgatt
     3841 gcatcatgtt gttctggaac tttagattct ggagttcctg gatgatgagg tgcatgcttt
     3901 tctgcatcat gtttcttctc ttctactcct tgttgaacgt tagggactac tggatctgga
     3961 tgtgcagata ctggtgctgt tttgttcttt tcatcgttca caccatttga ttggtttggt
     4021 gtcgttgggt ctggatgtgc agacactggt ttttggttgt tcattttatt gttattattt
     4081 ttgttattgt tattcatgga tgtttcctcc taagatttaa aatagtgatc taagattttc
     4141 gtttgtttca gataaaaacc gatagcgacg ccgatcgctg ccatgatcaa gatcaataag
     4201 gtcttgaaaa agcccacggt gatccataaa acggctaata tcaatccaag taccccaaag
     4261 atgataggga gcttgtatgt cgaaaatagt tctttcattt ttggtccccc cctattctac
     4321 tcgtttgcgg tcattgcttc gtttagctgg ttcttcaaag cctgtgtatt tcaccgatac
     4381 tttcactggc tcttttgttc ccaatatgtg ttgtacttca ttttcgattt ctttcatcaa
     4441 tgttcctgtt ttaccaatga gtgctgaagt tcgtttcaac tcccctttta cattgacggc
     4501 gatcttgttt ttcgttgctt tgacatttac ttttggtgaa tggatgaatt catcttcatg
     4561 caagtgagaa cgaaccattc cttcaattgc ttttttatcc aacgtcaact ccccatcacc
     4621 atgtttcagt agaaagtttc cgattgtttt tggataaaac aggaaaacaa gtaacgtgat
     4681 aattgtcaaa atagcaaatg caacggcaag ccaaaataca tattgttgca cgtatgaatt
     4741 agtgatggaa aagaatcgaa acttctctag ctgaaaaggc aatggatagg ttgcacttaa
     4801 tagtatcgca aacaaaagca cagatagcaa aagtaatgtt ccgatcactc cgatcgtttt
     4861 tacaccttta ttcattttcg cacctcctca taggggtgaa acgatcaact tcttataagt
     4921 tgatcgcttc tccaccagtt actccgtaaa tttgggctgt aacataactt gctttattcg
     4981 atgctaagaa aacataaact ggtgctaatt ctcctggctg acctgcacgt tctagtaaac
     5041 tttgctgacc aaattcaggg atcgaatcag aaggttgtcc ttcgtctagt tgtaatggtg
     5101 tccagattgg accaggtgct acaccattta cacggattcc tttaggtgca agctgttttg
     5161 ctaaaccgat cgtaaagtta gcgattgaag cttttgttgc tgcgtaatct aataaatgtt
     5221 cgcttggatt gaatgcttga acagaagttg ttgttacgat tgctcctcct gctgggatat
     5281 gcggaacagc ttctttcact aaagcaaaca tagcgatgat gtttatcata aatgtgtctt
     5341 tgacttgctc aagtggtaac tcagcaatag actgacaaga aatttgctgt cctgcgttta
     5401 gtaccatcgt atctagcccg ccaagttcac tcacggcttt atctatgatt tctttaggtg
     5461 cttgttcgtc acgaagatca aaaggtagta agacaatctt acgtcctgca actttcacgt
     5521 attttgctac ttcctctgca tctttttctt ctcctgggaa gaattgaaga gcgatatctg
     5581 ccccttctct agcaaatgca atcgctgctg cacgaccgat ccctgaatct ccaccagtga
     5641 ttagtacacg gcggttttct agttgattat tgccttcata ggattcttct ccacaatcag
     5701 gaacaggaat catattctgc tgtaaagctg gtgtatcctg atcttgttta ggaaatttct
     5761 ccgtatggta caagtttcgc gggtcgatta attttggttc tgtcattgct gtattcctcc
     5821 tagtttttaa attgtattgc caaaacaaat ttctttgttt gatacctgtt ttaaatagat
     5881 tctttaattt gtataatctt tccggtataa atggtaacct aaaactactg ctgctatcaa
     5941 agagccaaag actacgagta taaagcctag agcagaactt ttacccggta atcctcctgt
     6001 attcattccc caaatccctg cgaacaacgc cggaacggaa atgattaatg cagctgaatc
     6061 tagatatttc atgatatgat ttagattatt gtccatcatc ccgttgaata gatcaccaat
     6121 actctctaat aaatctcgat agatctcgat cattttttct gtttgtcgct gtctcaaatg
     6181 gacatcatag agtagctgtt catcgtctaa acgtttgatg aaagtttcag aattccataa
     6241 ttgatccatt gtttcccgtt gatctcttaa ggtatgatcg atatatacga tgtctctttc
     6301 aagatctgcc tgtttataca gttcttcatt ttccgttgtt ttacgagctg ctttatccaa
     6361 agcgtcaatt cgctttttca tctctcttag ttctttcacg taatgggtgt aaatatctaa
     6421 taatgtatac gtgatgatct tttcaaacga gtttaaatga tttccatgct ttgtcagcag
     6481 ccgatccaca aattcggttt ttctatcgat acaagtaatc aagagattat cggaaagtac
     6541 aaaagatatt ggcgttaatt ttttttcgat acgattatct gactgatcta gattaatcac
     6601 taccaaagaa aatggatgct ctagtttcgt ctcataaagg cgctccatcc gagaaacttc
     6661 ttccggatat tcggttccta cgaaaatatc atcaggaagc tcgaattctt cgattgcttg
     6721 ctcaatctct tcgggctttg cactatcaag tacgagccaa ttatattttt tttcatctgt
     6781 ttctattaca ttcttttctt catccacaaa gtagtatctc aatgtatacc tcctttttgg
     6841 actttctcga aactattagc tgaagaagca gttctttaat ttgatattat aatcgagtac
     6901 tgcgatttca ttttctaatg aaaagctgtc aagctgtttg ttgaagtcca tgttcaatcg
     6961 tttaccgaaa taattttgct ctaatttaga tgtatagatg tccaataatg gatgctcgat
     7021 tttagtcgca tattctccaa attccgaaac aaaaggaatg atttgaaaaa agctgccttt
     7081 tgcaaaagca cctttgtccc ttagaaatgg atcggaatca ttgataagcg cgagtgcgat
     7141 ttttgtattt tccccaacct tttcaattaa cgtctctact gcatttttag acattgtaat
     7201 ttccattcct actccccctc ttcagttgtt gtcttctatt atttaacaac tacatctgtt
     7261 tttggttgaa cttttgcata ttttggatat ttagggaatt tagctacaca acggaaagcc
     7321 gtcatccctg catatttttc gtaatatttc ttcgttggcc cttcaaaatc cttgctccac
     7381 ggtttcacat gtcctgaaaa atggataatg taaggattgt tgcgagtttc ttcgtattca
     7441 cgttcacctg ctgctgttgg atgttttttt gcttttgcca tgatatagcc ttgtgcattc
     7501 caacgcggat gaagtggtaa ccaacggtca tgtaagatag catttaatgc atcttggtca
     7561 tggaatcgta atttttctgg attatgttcg atgaaatcta atactttttg cgtaatattt
     7621 tcatctaacc attttttcac attgatcagc atcaaacctg agttgaaata acgcattgat
     7681 ttagctgaaa ttttcatttt ttccagtcgt tgatggaacc cagcgtcttc aacagctgct
     7741 acgattgaat caccgaagtc aagacgccat aatttagaga tatcttgtaa agcgatcatg
     7801 tcgcaatcca tatataagat acgttcaact tctgtgccgc ggaacaattc cggaatcgca
     7861 atacgataat aagctgtttc tggaatacga tcgctgatta ccacatttgt aaagaattct
     7921 ttattgattt tcaagaattc cacatctgag ttcattcgtg cgtttttcac tgagaagcgt
     7981 aaaagctgtt tactttcaaa atcgatatca tcgtcaatca catagaaccg aacaaattta
     8041 gacgggttgc aattatctaa tactgagaca aataatgcag ctaaatgtgg tacgaatttt
     8101 gtgttacaac ttgcgactac tgctatttct tttttattca ttgatctcgc ctactttcgt
     8161 tagagtactg tgtgctaatt tttttaagta taaattggta tacggatgat ctttcaacgt
     8221 attccaaggt ttatcatgtc ctgtaaagtg aacgatcgaa ggtttttcat ttccttcttt
     8281 atactgacgt tcatattttt catttggtgc tggatgacgt tcaaaaatca gcgaagtctg
     8341 catgttccat tttggatgaa gttgttccca atcttcataa agtacagcat tcaaagcatc
     8401 ttggtcgtga tagatgattc gatcgccatt ttcactgagg taatggatag ttttttctgt
     8461 aatttctttt ttattccatt gatcaatgtc aatcaccatc actcctgaat tgaagtacaa
     8521 gtcatcggaa tcgattccta atctttccaa agctttcact tggcctggat cgataatagc
     8581 gccgattgtt tttccattta acggctcatc atatagtttt acgatatcgt ctagtactaa
     8641 cacatctgaa tctaagtaaa ggactttctt ataatcctct ttagctaata agtttggtaa
     8701 agaaattctt aagtaagcgg tagttgtgat atggtcactg actaggaagt cttcgtagat
     8761 atctttttcc actgtcagga attggatact tgcattactt gaatatttat ttaccgtttc
     8821 ttccaatcct tctttactat attctgatag accatcgtca atcacataaa attttatgcg
     8881 tcttgtttta ttgcaatttt ctaatgcagt tgcgatcatc acgctcaagt aaggagcata
     8941 attttcgtct gaagcagtta ccacaggaac tacgccgtat ctcttctcca tcaaaatcac
     9001 tcctttttct aatctttttc taacctgttc tacatctaga atattcctca tttggtttta
     9061 aagcaaaata tatgctttgt tttcattgaa tattttaaaa tttttttatc acaaaacgca
     9121 tcggtaagcg ctaactattc gtttttcatg gctttttatt caaaggtatt gaccaaaaac
     9181 tactatatat agccaccttt ttcttaagaa tgttttttga aaaaattcat aatgacaaag
     9241 caagttagtt tcattttttt aaattctcat ttaatcttaa ggtaacagga aaatagatat
     9301 gtaaaaagaa agtaggaaga taaaatgaaa ctagcagcga ttgatttaga cggtaccttg
     9361 cttgatcgaa atggaaagat accagaagaa aatatccaag cacttcggaa atttgatgaa
     9421 aatggcggaa tcgtaaccgt cgctaccgga aggaacagca tttctgctaa agatattttt
     9481 tccgaattag gaatcggtgg ctatttgatt tcttccaatg gttcactgat tgctgaaatg
     9541 aaggatggaa aaatggcgaa agttctcaga cgttcaaaaa tagatagcga tacactgaga
     9601 aaagccttct tctatgctca agaagcgaaa atatccatca ttgctagccg agaaacccaa
     9661 gatgatcagc tgacattcga tgaatcaaat ctagtcaaag atgaccctta ttatgaacat
     9721 ttcaatttac agaaccattc ttttgatcaa attttaaata tgttggaaga tccttctctt
     9781 agctatctta aattagcatt aacagataag agtgaagaaa aactacagaa gctacaaaga
     9841 gatctgcaag cagatggtat ccaatcagta ttctctgatc cgcatttctt agaaatcacg
     9901 cctacaggta tcactaaagc tcattcactt gtttttctgg cggattacct tggaatcgaa
     9961 tcgagtgaca tcatggcttt tggcgatcaa gaaaacgaca ttgctatgtt gaattacagc
    10021 gggttgagta ttgcgatggg gaatgcacag cgacaagtaa aagaaattgc aaaccaagta
    10081 acactgacga atgaagaagc tggtgttgct tatattttaa aaaacttcta ttaaaaagaa
    10141 taaaatgacg atatgaatgt aggaggaaag aaaatgttag ttattattga tatgcaaaat
    10201 catattcttg atccaaacag cgaattttat attccagagt cagatcaatt ggcagaacgt
    10261 attgctcaaa gattagctaa agcacgccaa aataatgaat atattctctt cacacgggat
    10321 ataccagttg aactgaagga caaagaagaa gaacgagaag atttgcaatt gatccctctt
    10381 ctttctccac aagaaaatga acgagtgatt aaaaagtatt actttacgat tccacctgaa
    10441 actttagctg aaatccagga aactttattt gaaagcaaag aagaacaaaa agagatcgaa
    10501 gtagtcggga ttgaaacaaa cttgtgtgta ctctctaatc tgataggcct gcagagtgct
    10561 tttcctgaag cagacttttt tgttgatcca atgttagtct ccagtagaaa acacggagaa
    10621 tccgctttag agctgttgaa agatttcaac gtctctatcg tcgaatcata aaaatatcta
    10681 tcttcattta cgatatttaa cacacaataa aaaaagcacg cagaactgtt ttagtcctac
    10741 gtgctttttc tatcaaagta gattggcttt tttatgacac agagtgaaac ggcaggctca
    10801 cgacctcttt cttaaatggt gttcaatcag ctgaccctcg gtattagctt aacaaacggc
    10861 aaaatatgag gaactatttt tccgtttgtt aagctaatac tcaggtcaaa acgctgattt
    10921 cacaacctct tccttaaacg gtattcaaaa gcttgcatct gcagtaataa gaacagccaa
    10981 gtaaaaacat aagaagctgt ttttacttgg ctgttcttat tacttgatgc agggcagctt
    11041 tttcacaacc tc
//
LOCUS       DACDKM010000093        10510 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20834_2016
            SAMN12640370-rid9891663.denovo.093, whole genome shotgun sequence.
ACCESSION   DACDKM010000093 DACDKM010000000
VERSION     DACDKM010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640370
            Sequence Read Archive: SRR10040891
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10510)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10510)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742835.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742835.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:04:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,012
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 29 of 75
            Pseudo Genes (incomplete)         :: 33 of 75
            Pseudo Genes (internal stop)      :: 19 of 75
            Pseudo Genes (multiple problems)  :: 5 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10510
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640370-rid9891663.denovo.093"
                     /isolate="E20834_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            9..1127
                     /locus_tag="IV334_002199"
     CDS             9..1127
                     /locus_tag="IV334_002199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HAP8944253.1"
                     /translation="MFEKLRQSKLMFWSLELLIIAALIMISSEINFIFEPIGTFFSTL
                     FAPVLIAGFLYYLLNPIVKLLMKAKIKRIWAVAIVLLLLVAAIIWILLSVIPSLVQQI
                     SSLASNMPDFIKQVESWLKEVAELPLFKEVDINKYFEQLDISYGTIIQQFLSGVSNSL
                     GSIVGTIASATVVIITAPFILFYMLKDGEKLVPNIQQFFPEKRRNQIVELLGQLNKTL
                     ANYISGQAIECLFVGTFTFLGYMVIGVDYAFLFGVIAGLTNLIPYLGPYLGLAPAFLV
                     TVFNDPVKALLCCVVVLIVQQLDGNIIYPNVIGKSLKIHPLTIIIVLLVAGNIAGLLG
                     IFLGVPFYAICKTIISYIIKIVKEDKQNENKKKIATTS"
     gene            1255..2631
                     /locus_tag="IV334_002200"
     CDS             1255..2631
                     /locus_tag="IV334_002200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010767236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HAP8944254.1"
                     /translation="MNADPESQSLLAQILLLIVLTLINAFLAASEIAVVSINKNRIEQ
                     KADEGDVKSQKLLKILQNPNNFLSTIQVGITLVNILSGASLANTLSERLAPVLGGGTA
                     AKNIANIIVLAILTYVSIVFGELYPKRIALNKSEEVAKFTSGLIRLIGVVAKPFVWLL
                     SASTSLLARITPMTFDDEDSKMTRDEMRYMLENEGVLNNEELEMLQGVFSLDTKVARE
                     VMVPRTDAFMIDINDTVEENVNEVLSENYSRIPVYNEDKDKVVGILHTKNLLKAAHKF
                     GFDNLDIKKIMQEPLFVPETVFIDDLLYEMKKTQNQMAILLDEYGGVVGLATLEDLLE
                     EIVGEIDDESDEVENLYEKIDEHEYIIQGRMLIDEFNEAFDSDLHMSDVDTMAGYLIT
                     ALGMIPDEGEKLSFDVDNITLVSEEMEGSRILKIRVIFHDPEETEAEPEEERRYFRKE
                     FEDDEPRR"
     gene            3032..4612
                     /locus_tag="IV334_002201"
     CDS             3032..4612
                     /locus_tag="IV334_002201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HAP8944255.1"
                     /translation="MNNPKLKQQVDNRRTFAIISHPDAGKTTITEQLLLFGGAIRQAG
                     TVKGKKTGNFAKSDWMEIEKQRGISVTSSVMQFDYQGKRVNILDTPGHEDFSEDTYRT
                     LMAVDSAVMVIDSAKGIEAQTKKLFQVVKKRGIPIFTFINKLDRDGREPLELLEELEE
                     LLDIESYPMNWPIGMGKGLEGLYDIYHNRVEFYRPENYQDERLVELDEDGLLPENHPL
                     TENSLYEQVLEEVELIKEAGDAFNQEKIARGDQTPVFFGSALTNFGVQTFLETFVELA
                     PAPYGHKTQDDQMVSPYEEEFSGFVFKIQANMNPAHRDRIAFVRICSGTFERGMDVWL
                     ERTNKKLKLSNVTQFMADSRENVEKAVAGDIIGVYDTGNYQIGDTLFEGKLKVAYEEL
                     PSFTPELFMKVTAKNVMKQKSFHKGIYQLVQEGAIQLYKTYLTEEYIIGAVGQLQFEV
                     FQYRMLNEYNAEVIMSPMGHKIARWIDPEDLDEKMSSSRNILARDRFDQPLFLFENQF
                     AERWFADKYPNVKLKSLM"
     gene            4810..5033
                     /locus_tag="IV334_002202"
                     /pseudo
     CDS             4810..5033
                     /locus_tag="IV334_002202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287661.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein"
     gene            complement(5238..5558)
                     /locus_tag="IV334_002203"
     CDS             complement(5238..5558)
                     /locus_tag="IV334_002203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1827 family protein"
                     /protein_id="HAP8944256.1"
                     /translation="MKLVNVTNSHSRLVLNQLENTDAHLVKVYTAGNTTIVYTEAPEH
                     NEILLINDKRKIQPKEIEDAKNYFLDKIKDAVYHEDEIKVIELAGLVEISIPKHELGA
                     TLAV"
     gene            complement(5706..7940)
                     /locus_tag="IV334_002204"
     CDS             complement(5706..7940)
                     /locus_tag="IV334_002204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA domain-containing protein"
                     /protein_id="HAP8944257.1"
                     /translation="MLCQNCGKNEATIHLYATVNGQRTQLDYCQSCYQKIKNQQNGGL
                     TNMAQNDPFGFGSLDDLFRSMSRQMQQQGNYEQTPPTQFGGNNNFNGGQPPQGNTEGL
                     LGEYGINITEQARQGDIDPVIGRDDEIKRVIEILNRRTKNNPVLIGEPGVGKTAVVEG
                     LAQKIVDGDVPQKLMDKEVIRLDVVSLVQGTGIRGQFEERMQKLIEEIRQAENVILFI
                     DEVHEIVGAGSAGDGNMDAGNILKPALARGELQMVGATTLNEYRIIEKDAALERRMQP
                     VRVDEPTVEETISILKGLQKRYEDYHHVKYTDEAIEAAATLSNRYVQDRFLPDKAIDL
                     LDETGSKKNLTIQIVDPKTIEKKLQEAEEQKVLASREEDFEKAAYYRDQINKLQKMKE
                     RQLTEEETPVITEKDMEKIVEQRTGIPVGELKEKEQTQLKNLADDLKAHVIGQDNAVD
                     RVAKAIRRNRVGLNKQNRPIGSFLFVGPTGVGKTELAKQLAYELFGSQDSMIRFDMSE
                     YMEKHSVSKLIGSPPGYVGYEEAGQLTEKVRRNPYSLVLLDEVEKAHPDVLHMFLQIL
                     DDGRLTDAQGRTVSFKDTIIIMTSNAGTGKVEANVGFGAAREGVTRSVLNQLNNYFTP
                     EFLNRFDGIIEFSALSKENLMTIVTLMLDDVNQMLAAQQLHIEVPTNVKEKLVDLGYD
                     PSMGARPLRRTIQEQIEDGIAEFYLDHPSIHELKAKLDKDGKIIVTSKPERLAKESAE
                     ETAE"
     gene            8419..8685
                     /locus_tag="IV334_002205"
     CDS             8419..8685
                     /locus_tag="IV334_002205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010773230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocarrier protein HPr"
                     /protein_id="HAP8944258.1"
                     /translation="MEKKEFHVVAETGIHARPATLLVQTASKFNSDVNLEYKGKSVNL
                     KSIMGVMSLGVGQGSDVTITAEGADEADAMAAIVETMKKEGLSE"
     gene            8685..10412
                     /gene="ptsP"
                     /locus_tag="IV334_002206"
     CDS             8685..10412
                     /gene="ptsP"
                     /locus_tag="IV334_002206"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HAP8944259.1"
                     /translation="MVEMLKGIAASDGVAVAKAYLLVQPDLTFSKATVEDTAAEEARL
                     DAALAKSTEELQQIREKAAQSLGEAEAQVFDAHLMVLSDPEMVGQIKQNIKDNSVNAE
                     SALKEVTDMYIGMFEAMEDNAYMQERAADIRDVAKRILAHLLGVTLPNPSMINEEVVV
                     VAHDLTPSDTAQLDRNFVKAFVTDIGGRTSHSAIMARSLEIPAIVGTKEITAKVKEGD
                     ILAVNGIEGDVIIDPTDEQKAEFEKAGADYAAQKAEWEKLKNAETVTADGKHFELAAN
                     IGTPKDLVGVHNNGGEAVGLYRTEFLYMDSPDFPTEDDQYEAYKAVLEGMEGKPVVVR
                     TMDIGGDKELPYLQLPHEMNPFLGYRALRISLSEQGDEMFRTQMRALLRASVHGNLRI
                     MFPMVATLKEFRAAKAIFEEEKQKLISEGKEVSDTIQVGIMIEIPAAAVLADKFAKEV
                     DFFSVGTNDLIQYTMAADRMNERVSYLYQPYNPSILRLIKNVIDAAHAEGKWAGMCGE
                     MAGDQTAVPLLVGMGLDEFSMSATSILKTRSLMKRLDTAKMAELADRALKECDMMEEV
                     VELVHEYVK"
BASE COUNT         3343 a         1836 c         2148 g         3183 t
ORIGIN      
        1 gaaatctatt gtttgaaaaa cttagacagt ctaaactaat gttttggtcg cttgaattat
       61 tgatcatagc tgcattgatc atgatttcat cagaaataaa ctttattttc gaaccgatcg
      121 ggacattttt ttccacgtta tttgcacctg tactgattgc aggctttctt tactatttat
      181 tgaatcctat tgtcaagctg ttgatgaaag caaaaatcaa aagaatatgg gctgtcgcaa
      241 ttgtgctgct ccttttagtt gcagcaatta tttggatttt acttagtgtc atcccaagtc
      301 tagtacagca aatttcctca ttggcatcta atatgcctga tttcatcaaa caggtagaaa
      361 gctggttaaa agaggtagcc gaattgcctt tgttcaagga agtagatatc aataaatatt
      421 ttgagcaatt agacatttct tacgggacaa tcattcagca attccttagt ggagtatcca
      481 atagtttagg ttcgatcgtt ggaacgatcg cttcggcaac cgtagtaatc attacagcac
      541 cattcatctt gttctatatg ttgaaagacg gagaaaaact agtgccaaac atccaacagt
      601 ttttcccaga aaaaagaaga aaccaaattg tggagttatt ggggcaactg aataaaacat
      661 tggctaatta cattagcgga caagcaatcg aatgtttatt cgttggtacc ttcacatttt
      721 taggatatat ggtaattggt gtggattatg ctttcttatt tggggtcatt gcgggattga
      781 caaacctaat tccatattta ggaccttatc taggactagc acctgcattt ctagtaacag
      841 tcttcaatga tccggtaaaa gcattacttt gttgtgtggt tgtgctaatc gtccaacagt
      901 tggatgggaa tatcatttat cctaatgtaa ttgggaaatc actgaaaatc catccattaa
      961 cgatcattat cgttcttcta gtagctggaa atattgccgg attgcttggt atcttcttag
     1021 gtgttccttt ttacgcaatc tgcaagacga tcatttccta tataatcaaa atcgtcaaag
     1081 aagacaagca aaatgaaaac aagaagaaaa ttgctacgac ctcgtaggta gttttagtac
     1141 acttgttttt attgcaaaga aaattgcttt gtgataccat tttaacgtgg ctatagtgcc
     1201 acgttttttt ctatcgtaaa ctaaaaatta attttataag gagagaatgc gattatgaat
     1261 gctgaccctg agagtcagtc gctgttagcg cagattttat tattgatcgt tttgacattg
     1321 atcaatgctt ttttagcagc ttccgaaatt gctgttgttt cgatcaataa gaatcgaatc
     1381 gagcaaaaag ctgatgaagg agatgtgaaa tctcaaaagc ttttaaaaat attgcagaat
     1441 ccaaataatt tcctatcgac aatccaagta ggaatcactt tggtcaacat tttgtctggt
     1501 gcttcactag ctaatacttt atcggaacgg ttagctccgg tattaggtgg cggcacagcg
     1561 gcaaaaaata ttgcaaatat cattgtgctg gctattttga cctatgtatc gattgttttt
     1621 ggagaattgt accctaaacg tattgcttta aataaatcag aagaagtcgc aaagttcact
     1681 tcagggctga tacgcttgat tggtgtagta gctaagccct tcgtctggct gctttctgct
     1741 tccacaagtt tattggcgcg tatcacgccg atgacttttg atgatgaaga ttcaaaaatg
     1801 accagagatg aaatgcgtta tatgcttgaa aacgaaggcg tattgaataa tgaagaatta
     1861 gaaatgctcc aaggggtttt ttctcttgat acgaaagttg cccgagaagt catggtccca
     1921 cgaacggatg catttatgat tgatatcaac gacactgtgg aagaaaatgt gaatgaagtt
     1981 ttatccgaaa actattcaag aatccctgtt tataacgaag acaaagataa agtagtcgga
     2041 atccttcata caaagaattt actaaaagct gcccataaat tcggctttga taatttagat
     2101 atcaagaaaa tcatgcagga gcctttattc gttcctgaaa cggtctttat tgatgatctg
     2161 ttatatgaaa tgaaaaagac gcagaatcag atggctattt tattagatga atatggcggc
     2221 gtcgttgggt tagcaacact agaagacctt ttagaagaaa tcgtcggaga aatcgatgat
     2281 gaatcagatg aagttgagaa tctttatgaa aaaatcgatg aacacgaata tatcatccaa
     2341 ggacgtatgc tgattgatga gttcaatgaa gcttttgaca gtgatctgca tatgagtgat
     2401 gtagatacga tggctggtta tttgattaca gcattaggca tgattccgga tgaaggcgaa
     2461 aagttatcgt ttgatgtcga taatattacg ctggtttctg aagaaatgga aggttctcga
     2521 atccttaaaa tccgtgtcat ttttcatgat ccggaagaaa cagaagcgga acctgaagaa
     2581 gaacgaagat acttcagaaa agaattcgaa gatgacgagc caagaagata attggaaact
     2641 tggttatgaa agacgtgttg cttgaataaa caagcaacac gtcttttctt ttagtcagct
     2701 gacaaaaaaa tatttgactc ttatggtttg gtggcttttt cattcgctga atccatgcta
     2761 tactgattca gttgtagtag ttgacaaagg ttgtgaaaaa gctatcctgc attaagtaat
     2821 aagagcagcc aagcaaaaac agcttttcgt atttttggct tgggtgggct tattgctgat
     2881 aggctgtctc tagaacgccg tttactcgga aaaagagact gctacacggc ttttgtcaca
     2941 gccctatatg aaattgtctt tctttaagag atcggacaat ctgtgcctag agaaaatata
     3001 aataattgaa tggaagaaat ggagttaggt tatgaataat ccaaaattaa agcaacaagt
     3061 ggacaatcgc agaacttttg cgatcatttc ccatccggat gctgggaaaa caacgattac
     3121 agaacaatta ctattgtttg gtggggctat ccgtcaagcc gggaccgtaa aaggcaaaaa
     3181 aacaggtaat tttgccaaat cagactggat ggaaatcgag aaacaacgtg ggatttctgt
     3241 tactagttct gttatgcagt ttgattatca agggaaacga gtaaatatct tggatacacc
     3301 aggacatgaa gatttttctg aagatactta tcgtactttg atggccgtgg atagtgcggt
     3361 catggtaatc gatagtgcaa aagggatcga agcacagaca aagaaattat tccaagttgt
     3421 aaaaaaacgt ggcatcccga tatttacttt tatcaacaaa ctagatcgtg atggtcgtga
     3481 accattggaa ttgttagaag aattagaaga gctattagat atcgaatctt atccgatgaa
     3541 ttggccaatc ggtatgggta aagggctaga aggtctttat gatatctacc ataatcgtgt
     3601 agagttttat cgtccggaaa attatcagga tgaacgatta gtagaattgg atgaagatgg
     3661 actgcttccc gaaaatcatc ctctaactga aaattcctta tatgaacaag tcttagaaga
     3721 agtagaacta atcaaagagg ctggagatgc atttaatcaa gaaaaaattg ctcgtggcga
     3781 tcagacacca gtattttttg gttctgcatt gactaacttc ggtgtacaga cttttttaga
     3841 gactttcgtt gaattggcac cagctcctta tggacacaaa acacaagatg atcagatggt
     3901 aagtccttat gaggaagaat tttcaggttt cgtattcaag atccaagcaa atatgaatcc
     3961 agcccatcgt gaccgaatcg cttttgtccg aatctgttct ggaacattcg aacgaggaat
     4021 ggatgtctgg ttggaacgta caaataaaaa actgaaatta agcaatgtca cacaatttat
     4081 ggcagattcg agagaaaacg tcgaaaaagc agtagcaggc gatatcatcg gtgtatatga
     4141 tacaggcaat tatcagattg gggatacatt gttcgaagga aaactaaaag ttgcttatga
     4201 agaactccct tcatttactc cagagctgtt tatgaaagtg actgcaaaaa atgtcatgaa
     4261 acaaaaatcc ttccacaaag gaatctatca attagttcaa gaaggagcga ttcagcttta
     4321 taaaacatat ctaacggaag aatatatcat tggagctgtt ggtcagctac aattcgaagt
     4381 attccaatat cgtatgttaa atgagtataa cgccgaagta atcatgtctc caatgggaca
     4441 caaaatcgct cgctggatcg atccagaaga tttggatgag aagatgagtt caagtcgcaa
     4501 tatcttggct cgtgaccgtt tcgaccaacc gttgttctta ttcgagaacc aatttgcaga
     4561 acgctggttt gcggataaat atcccaatgt aaaattaaaa agtttgatgt aaagaagtct
     4621 tgacacaagc tgccctgcat caagtaataa gaatagccaa acaaaaacag cctctcatat
     4681 tttgtttaga gccgtgacgg attttccgtg acggctcttt ttactatatt aaaatcatag
     4741 aaatgtctaa tcaaaaaaat ataaaaatta ttttgcgtac atatgattaa agaaaggaga
     4801 gtgtgacaca tggaaacaga atggcaaaga cgtgaaagaa aaaaacagaa aaagaagaga
     4861 tgttacgatt atgtcgatta ttagatgtaa aagactggga caaaaacgag caaattgcac
     4921 aaaaagtacg atcaatcatc cataaagagg aaaaagttaa aaataaagac acgcgtagaa
     4981 ctgaaaatga aaagagaaaa gtacaaaaga aaagagaaaa atcaaaaagc tagaaagaaa
     5041 aagaggctga gacagaagcg tttaactcca agaaataagc tggaattcac aaaaatttga
     5101 aaagcaattt tcgtgaattt cagcttattt ccgaaggagt tgcttctgct ttcgccgttt
     5161 atacgtgttt agagcgtgag acaaaagtgt tctttacttt tgtcccacgc tctttaatta
     5221 ttatgttcaa aatttgttta cacggctaat gtagcgccta attcatgttt tggaatcgag
     5281 atttcaacaa ggcctgccaa ttcgattact ttgatttcat cctcgtggta aacggcgtct
     5341 ttgattttat ctaagaaata atttttagca tcttcgattt ctttcggctg gattttacgt
     5401 ttgtcattga tcagcagaat ttcattatgt tcaggggctt cagtgtagac aatagtggta
     5461 tttccggctg tgtagacctt gactagatgg gcatcagtgt tttcaagctg gtttaataca
     5521 agacgggaat ggctgtttgt tacatttacc aatttcatgg cgctcacctc ttttatgtat
     5581 ttttaattgc ttgtttctct tattataatt tcttttttat aaaatgaaca gtattttgac
     5641 ttttctgaaa aaagaatgag acggcatttc tcaaagaaat accgtctcac gctataaaac
     5701 tggatttatt cagccgtttc ttcggctgat tctttagcca aacgttctgg tttagaagta
     5761 acgatgattt ttccatcttt atccagtttt gctttcaact catggataga agggtgatca
     5821 agataaaatt cagcgatacc atcttcgatt tgttcttgga tcgtgcgtcg aagaggacga
     5881 gcacccatag atggatcata gcctaagtca actaattttt ctttgacatt cgtaggaact
     5941 tcgatgtgaa gttgctgtgc tgccagcatt tggttgacat cgtctagcat tagtgtgacg
     6001 attgtcatca agttttcttt tgataatgca ctgaattcga tgatcccgtc aaaacggttc
     6061 agaaattctg gtgtaaagta gttgtttaat tggttcaata cagaacgagt aacaccttcg
     6121 cgtgccgcac caaaaccaac gtttgcttct actttaccag ttccggcatt acttgtcata
     6181 atgatgatcg tatctttaaa gctgaccgtt cttccttgtg catctgtcaa tcgaccatca
     6241 tctaagattt gcaagaacat atgcaaaaca tctggatgag ctttttctac ttcatctaac
     6301 aagacaagac tgtaaggatt tctccgtact ttttctgtta attgaccagc ttcttcgtat
     6361 ccgacataac ctggaggaga accaatcaat ttagagacac tatgtttttc catatattca
     6421 gacatatcga aacgaatcat tgaatcttgt gaaccgaata gttcgtaggc aagttgtttc
     6481 gcaagctctg ttttacctac accagttggt ccaacaaata agaaggagcc gattggacga
     6541 ttctgtttgt ttaatccaac acggtttcgg cgaatcgctt tcgctactcg atctacagcg
     6601 ttatcttgtc cgatgacatg agctttcaag tcatctgcta agtttttcag ttgtgtctgt
     6661 tctttttctt ttaattcacc cactggaata cctgtccgtt gttcaacgat tttttccata
     6721 tctttttcgg taatcacagg tgtttcctct tcagtgagct gacgttcttt cattttttga
     6781 agtttattga tctgatcgcg ataataagct gctttttcga aatcttcttc tcgagaagct
     6841 aaaacttttt gttcttctgc ttcttgcagt tttttctcga ttgttttagg atcaacgatt
     6901 tggatcgtca agtttttctt agaacctgtt tcatctagca aatcgatggc tttatccggc
     6961 aagaagcgat cttggacgta acgattggat aaagttgcgg ctgcttcgat ggcctcatca
     7021 gtatatttta catgatgata gtcttcataa cgtttttgca aacctttcaa aatactgatc
     7081 gtttcttcga cagttggttc atctacccgt acaggttgca tacgacgttc taaagcagcg
     7141 tctttttcga tgatgcggta ctcatttaag gtagtagcac caaccatttg caattctcct
     7201 cgagctaaag ctggttttaa aatattacca gcatccatat ttccatcacc agcagaacca
     7261 gcaccgacga tttcgtgaac ttcatctatg aataggatca cattttctgc ttgacggatc
     7321 tcttcaatca atttttgcat acgttcttca aattgtcctc gtataccagt accttggact
     7381 aaagatacga catctaaacg aatgacttct ttgtccatta atttttgcgg tacatcacca
     7441 tctacgattt tttgtgctaa accttcaaca actgctgttt taccaacacc tggttctcca
     7501 atcaagacag gattgttctt ggttcggcga tttaaaattt caatgactcg tttgatttcg
     7561 tcatcgcgtc cgatcacagg atcgatatca ccttgtcgtg cttgttctgt gatattgatt
     7621 ccgtattctc ccaataaacc ttctgtattg ccttgaggtg gctgaccacc attgaagttg
     7681 ttgtttccgc caaattgtgt tggtggtgtt tgctcataat ttccttgctg ttgcatttgg
     7741 cgagacatgg aacggaaaag atcgtccaaa ctgccaaatc caaatggatc attttgggcc
     7801 atattagtca gacctccatt ttgttgattt ttgatttttt gataacagct ctgacagtaa
     7861 tcgagttgtg tccgctgacc attgacagtg gcatacaagt gaatcgttgc ctcattcttg
     7921 ccgcagtttt gacaaagcat gaatcattca cgtcctttca caaaaatggg tacatccata
     7981 ttttacatct cgtttttcat gaggtaaaag aataacccct agttttttaa gagatttatg
     8041 aggtagaaaa ggtgaaatct ctattgacca ttcctgacct ttgactctta ttatactaaa
     8101 aataaaaagg gaagcaagta attcgtttca tatgaaaaca aagagggtca aaggaaagaa
     8161 tgataaacgt caggagaaga gaaagcgaca agaaaaagga aataaacacg tttgttataa
     8221 aatgtgaaga aaaggatgaa aaagaatccg gaataatttg aatactttta tctgaaattg
     8281 aatgaaaact gttgtatcta acgaaaaaaa atgttaatct ttacagatga aagggtggca
     8341 tttgttagga ttctctgaat tactaaaaaa gaaaaccctc acataaaaac aacactcact
     8401 taaaggagac cgattaatat ggaaaagaaa gaatttcacg tagtagcaga aactgggatc
     8461 cacgctcgtc cagctacact attagtacaa acagcaagca aatttaactc tgatgtaaac
     8521 ttagagtata aaggtaaatc agtaaacttg aaatcaatca tgggcgttat gtctttaggt
     8581 gtaggtcaag gttctgatgt tactattact gcagaaggtg ctgacgaagc tgatgcaatg
     8641 gcagcaatcg ttgaaacaat gaagaaagaa ggcttatctg aataatggtt gaaatgctaa
     8701 aagggattgc cgctagtgac ggtgtagccg ttgcaaaagc ttacctgcta gttcaaccgg
     8761 atttaacatt cagtaaggca acagtagaag atactgcggc tgaagaagct cgtttggatg
     8821 ctgcattagc taaatctact gaagaattgc agcaaattcg tgaaaaagca gcgcaaagct
     8881 taggtgaagc agaagcgcaa gtatttgatg cccatttgat ggttctttca gatccagaaa
     8941 tggtaggtca aatcaagcaa aatattaaag acaacagcgt aaatgctgaa tcagcgctaa
     9001 aagaagtaac ggatatgtat atcggtatgt tcgaagcaat ggaagataat gcttatatgc
     9061 aagagcgtgc tgcagatatt cgtgacgttg ctaaacgtat tttagcgcac ttattaggcg
     9121 ttactttacc aaatccatca atgatcaacg aagaagtggt cgttgttgca catgacttga
     9181 ctccaagtga tacagcacaa ttagatcgca attttgtaaa agcgtttgta acagacattg
     9241 gtggacgtac ttcacattct gctatcatgg cacgttcttt agaaattcca gcaatcgttg
     9301 ggacaaaaga aattactgct aaagtcaaag aaggcgatat tttggctgtc aacgggatcg
     9361 aaggcgatgt aatcatcgat cctacagatg aacaaaaagc tgaatttgaa aaagcaggtg
     9421 cagattacgc agcacaaaaa gcagaatggg aaaaattaaa aaatgcagaa actgtcacag
     9481 ctgacggcaa gcattttgaa ttagctgcaa acatcggaac accaaaagat ctagttggtg
     9541 tccataataa tggtggagaa gcagttggtc tgtaccgtac tgaattcctg tatatggatt
     9601 ctccagactt cccaacagaa gatgaccaat atgaagctta caaagctgta ttagaaggta
     9661 tggaaggaaa acctgtagtt gttcgtacaa tggacatcgg tggagataaa gaactaccat
     9721 atctgcaatt acctcatgaa atgaatccat tcttaggata tcgtgcattg cgtatcagtt
     9781 tatctgaaca aggcgacgaa atgttccgta cacaaatgcg tgcattgctt cgtgcatctg
     9841 ttcatggtaa tttacgtatc atgttcccta tggtagctac actcaaagaa ttccgcgcag
     9901 caaaagcgat ctttgaagaa gaaaaacaaa aattgataag tgaaggcaag gaagtatctg
     9961 atacgatcca agtcggcatc atgatcgaga ttccagcagc agcagtctta gcagacaagt
    10021 ttgcaaaaga agtagatttc ttctctgtag gtacgaacga tttgattcaa tacacaatgg
    10081 cagcagaccg tatgaacgaa cgcgtttctt acttgtacca accatataac ccatcaattt
    10141 tacgtttgat caaaaatgta attgatgcag cacacgctga aggaaaatgg gcaggtatgt
    10201 gtggagaaat ggctggagat caaacagctg ttccattgct agtaggtatg ggtctagatg
    10261 agttctcaat gagtgcgaca tctattctta aaacgcgtag tttaatgaaa cgcttggaca
    10321 cagcgaaaat ggctgaactt gctgatcgtg cattgaaaga atgcgatatg atggaagaag
    10381 tcgttgaact tgttcatgaa tatgtaaaat aaacaacgta tcaaaagctg gtgtggcttg
    10441 taaaagagct acgccagttt ttttgttctt ttttaagaaa acgtttttgt cagtaagaat
    10501 agacaaaaat
//
LOCUS       DACDKM010000094        10498 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20834_2016
            SAMN12640370-rid9891663.denovo.094, whole genome shotgun sequence.
ACCESSION   DACDKM010000094 DACDKM010000000
VERSION     DACDKM010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640370
            Sequence Read Archive: SRR10040891
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10498)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10498)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742835.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742835.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:04:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,012
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 29 of 75
            Pseudo Genes (incomplete)         :: 33 of 75
            Pseudo Genes (internal stop)      :: 19 of 75
            Pseudo Genes (multiple problems)  :: 5 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10498
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640370-rid9891663.denovo.094"
                     /isolate="E20834_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(219..704)
                     /locus_tag="IV334_002207"
     CDS             complement(219..704)
                     /locus_tag="IV334_002207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl-tRNA synthetase associated
                     domain-containing protein"
                     /protein_id="HAP8944260.1"
                     /translation="MSRATEQEAYELLKKLGISFQKVDHPAITSVKNVPFSLPGPQVK
                     NLLLKSRKGKQIYLVILPDEKHADLKQLAEQLKEKRLSFVSEEQLPDLLGVPAGTVTP
                     LALMHDKEKKIQVVIDAAIDRQDTVGFHPNINTTTLIMDFSDFEKILVYLEHLPVYEN
                     L"
     gene            1184..1825
                     /locus_tag="IV334_002208"
     CDS             1184..1825
                     /locus_tag="IV334_002208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HAP8944261.1"
                     /translation="MEYLIEVMPQLLVGALTTLKLFLFTLIGSLPLGIVLALGLSLHF
                     KPLRYLLQTYVWVMRGTPLLLQLIFIFYGLPTIGIVFDRFEAALIAFILNYAAYFAEI
                     FRGGIQSIPQGQYEAAQVLQLSPFQTVTKIVIPQVTKIVLPSVGNEVINLVKDSSLIY
                     ILGLGDVMRAGKIAMERDATLLPLLGVAIIYLTLTGILTIIMKQVEGKLNYYR"
     gene            1844..2479
                     /locus_tag="IV334_002209"
     CDS             1844..2479
                     /locus_tag="IV334_002209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HAP8944262.1"
                     /translation="MIELKKVNKAFGEKKIIKDLNLIIPDGKTLAIVGPSGGGKTTLL
                     RILAGLESSDSGEFYLDGVPFNPFELKMKEQVVGVVFQDFQLFPHLNIFENITLAPKM
                     VLKQTKEEYTKKAEELAASLGISDLLNIYPYQLSGGQKQRVAIARALAMNPRVLAYDE
                     PTSALDPELRQQVERLILSLKEDGVTQIIVTHDLVFAENVGDQLLKVSPIH"
     gene            2497..3312
                     /locus_tag="IV334_002210"
     CDS             2497..3312
                     /locus_tag="IV334_002210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HAP8944263.1"
                     /translation="MKKTSFLFALIAGLLLFAGCSNKKTSADNWATINEEKKIVIGLD
                     DTFVPMGFRDKDGKLTGFDIDLAKAVFEQYGITVDFQPIDWSMKEFELNNGTIDLIWN
                     GYSKTPAREKKVQFTKPYMENDQVLITPKESGITNFGQMNNKRLGAQNGSSGYDVFTK
                     QPEVLKEIVKNQDAVLYDSFNEALIDLKSRRVDGLLMDKVYADYYLKQRNELEDFNIT
                     KGSYESEDFAVGARKSDSTLVKKINEALDQLEKTGEFQKISDKWFGEDVTPRN"
     gene            3631..5067
                     /locus_tag="IV334_002211"
     CDS             3631..5067
                     /locus_tag="IV334_002211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HAP8944264.1"
                     /translation="MEHKELTSFPKDFLWGSASAAYQVEGAWQEDGKGESVWDRFVRI
                     PGKTFKGTNGDLAVDHYHRYKEDIALMKEQGLKAYRFSVAWTRIFPNGRGEVNQAGLA
                     FYERLIDELIENDIEPMLTLYHWDLPQALQDEYNGWESRQIIEDFTHYAETLFEAFRG
                     KVHYWISLNEQNIFTSLGYLLAAHPPGVTDPKRMYEVNHIANLANASVINKFHELEMP
                     GKIGPSFAYTPNYPIDSNPENVLAAENAEDLMANYWMDVYMWGKYPIAAMRFLEEKGW
                     APTIEAGDAELLESAKPDFLGINYYQTATNAFNPLNGVGAGKMNTTGKKGSSEETGVP
                     GMYKKVENPFVERTNWDWEIDPEGLRIGLRRITSRYRMPVLITENGLGEYDKLTEDKQ
                     IHDDYRINYLQSHVKAIKEAISDGAEVLGYCTWSYTDLLSWLNGYQKRYGFVYVDQDE
                     TQEGSLERYKKDSYYWYQKLIEENGENL"
     gene            5256..7703
                     /locus_tag="IV334_002212"
     CDS             5256..7703
                     /locus_tag="IV334_002212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HAP8944265.1"
                     /translation="MAQKKRPTKKRKKTKKTKKQKQQQEQMLTIGVGFALILFAVFGF
                     LKLGFLGILIANGFRIIAGNTYQILCLLLAVLGFWIVIKNTEFSIGKNRRWFGGILFY
                     FGILLLLHAHLFGKLHTGEPNIMGTTWDLLASDIKQSQVDNNVGGGMIGAILYHFTYF
                     LIAQPGSYLAAVLLLAGGAFLFSNLEGYQLLNGIQSIGERVQELLEGDPAKQARKQAA
                     KEERMKQRAEAKEARRLAAKEAAEKEAAEYEKKKAVSQQRENTADEWQEPENTEPEQL
                     SFVPIDSFQENIHPANLEKPVPDTPKQTNTAEGFSDELPEDDGTSLEFEIEAEQENQD
                     YELPTVDLLDSIPTVDQSDEYKKIEKNIGVLEQTFQSFGVDAKVVKASLGPSVTKFEV
                     QPAVGVKVSKIVNLTDDIALALAAKDVRMEAPIPGKSLIGIEVPNGKISMVSFREIIE
                     AQPNHPDKLLEVPLGRDVSGRVQTADLSKMPHLLVAGSTGSGKSVAINGIITSILMRA
                     KPHEVKLMMIDPKMVELNMYNGIPHLLTPVVTNPRKAAQALQKVVQEMEERYEKFAAT
                     GVRNISGYNEFVQQKNLENGTKHPTLPFIVVIVDELADLMMVASNEVEDAIIRLAQMA
                     RAAGIHMILATQRPSVDVITGIIKANVPSRMAFAVSSGTDSRTIIDSNGAEKLLGRGD
                     MLFLPMGENKPIRVQGAFISDHEVERVVQFVTDQQEAHYEEKMMPTDEVETTGAPEQP
                     QDELFEEAKALVVEMQTASISLLQRRFRIGYNRAARLVDELEAHGVVGPSEGSKPRKV
                     FIEQEETSETLPDDNDM"
     gene            7933..9000
                     /gene="rlmN"
                     /locus_tag="IV334_002213"
     CDS             7933..9000
                     /gene="rlmN"
                     /locus_tag="IV334_002213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HAP8944266.1"
                     /translation="MEKQSIYGLTNEELINWFIENGEKKFRAAQVWEWLYQKRVSNFT
                     EMTNLSKQLIEKLSEHFIINPLKQMVVQEASDGTVKYLFELPDKNMIETVLMRQEYGL
                     SVCVTTQVGCNIGCTFCASGLLKKNRDLTAGEIVAQIMMVQHYFDERNLGERVSHVVV
                     MGIGEPFDNYDNVMDFLHIINDAKGLAIGARHITVSTSGLAHKIKEFANNGLQVNLAI
                     SLHAPNNEVRTSIMRINRSFPIEKLMEAVDEYLEKTNRRITFEYIMLNQVNDRPEHAQ
                     QLADLLKDKKKLAYVNLIPYNPVSEHDQYSRSPKADVLRFYDVLKKNGVNCVIRKEHG
                     TDIDAACGQLRSKQMKKTVAK"
     gene            complement(9193..10296)
                     /locus_tag="IV334_002214"
     CDS             complement(9193..10296)
                     /locus_tag="IV334_002214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HAP8944267.1"
                     /translation="MSQSVFLPNYTIGTDAYEKANGIVKEYGTKVVLIGGKTALSKAE
                     NKIKVALTQEIEVVASLWYGGNSTYGNIEQLIQHESVQKADVLFAVGGGRACDTVKVI
                     GEKLAKPVFTFPTIGSNCSPVTAVCVIYNEEGVMDGLFFPSKPPVHTFIDTAIIAEAP
                     DKYLWAGIGDALSKEVESTFSARGDDLGYTDRLGIQIGKMCIDRLLTYGTQALKDSAD
                     DRPSYAIEQVVSDIIVTTGLVSVLVNNNYNSAMAHSFYYGTTVLEKMEGYLHGASVSY
                     GVLTLLMMDQQFDLFDKIYVFMKKNQLPVSLTDMNLNVTTDLEAILDKAMATNDIKHV
                     PYEITREKFKQAILDVETYHATQESQQGYKKIS"
BASE COUNT         3469 a         1794 c         2283 g         2952 t
ORIGIN      
        1 cgatttataa tcgaaaaaag aagcattttt atatatgctt cttttttcgt agaatcaata
       61 aatatggatt tggctaacta tggaagaata ctcgtattag ttttcagcgt tcatactagt
      121 tgttacattg tttgattggc caaaagaatt tctgatatat tttgtgctaa ataaagaaaa
      181 agacgcttga attgggaaat aagaaaacta ctctatcatt acaaattttc ataaactggc
      241 agatgttcta agtaaacaag tatcttttca aagtcggaaa aatccataat caacgtagtg
      301 gtattgatat ttggatgaaa gcctacggta tcttgacgat caatagcagc atcgatcacg
      361 acttggattt ttttctcttt gtcgtgcatc aatgctaatg gagtcactgt acctgctgga
      421 acgcctagga ggtcaggaag ctgttcttca gaaacaaaag ataagcgctt ttcttttaat
      481 tgttcagcta gttgttttaa atcggcgtgc ttttcatcag gcaagatgac aagatagatt
      541 tgttttcctt ttcttgattt caataataga ttttttactt gcggaccagg tagggaaaaa
      601 gggacatttt tgactgacgt aatcgcagga tgatcgactt tttgaaaaga gatacccagt
      661 ttttttagta gttcataagc ttcttgttcg gttgcacgac tcatcgcgca tcaccctcct
      721 ttttttatta gtgtatcata aaaaagagtt gacaaatgaa aaataatcat gtaaattaca
      781 cgtaaagtta aaacaaatga catcgaaaag aagagtagcc gttaggtgtt tttagagagt
      841 ctctggttgg tggaaagagg cagcagttaa ccgtgaaaca catcttggag tcgattttct
      901 gaagcgtata gtaggaaaat ccggggagcc cgttacagct gcggtcgttg ctagatgacc
      961 agataaggct ggcttttccg tgaggagtcg gtaaaaaaag gtggaaccac gtgattaacg
     1021 tcctttgatc ttcggatcaa aggacttttt gttttcatct gaacggcaac gactgtgtga
     1081 ggttttttct gcaaggaaaa aataaacaga ggtggaacca cgtataatac gtcctcttgg
     1141 catttgccaa gaggattttt ttattttaaa ggaggaaaag aagatggagt atttgattga
     1201 agttatgccg caattattag taggagcact cacaacattg aaattatttt tatttacctt
     1261 gattggctca ttaccattag ggattgtttt agcattagga ttgtctttgc attttaagcc
     1321 gctacgctac ttgcttcaaa catatgtatg ggttatgcgg ggaacaccgc tgttgttgca
     1381 actgattttt attttttatg ggttgccgac gattgggatc gtttttgatc gtttcgaagc
     1441 agcactcatt gcctttatct taaactatgc agcttatttt gcagagatct ttcgtggagg
     1501 gatacaatcg attcctcaag gtcaatatga agcagcgcaa gttttgcaat taagtccctt
     1561 tcaaacggtg acgaaaatcg tcatcccaca agtgacaaag attgttttac cttctgtagg
     1621 gaatgaagtt atcaacttgg tcaaagattc ttccttgatc tatatattag gtttaggtga
     1681 cgtaatgcgt gctgggaaaa tcgcgatgga acgcgacgcg actttactgc ctttgctagg
     1741 cgtggcaatc atctatctta cattgactgg tattctaaca atcataatga aacaagttga
     1801 aggaaaatta aattattacc gctaactgat aggaggaaga aatatgattg aattgaagaa
     1861 agtgaacaaa gcatttggtg aaaaaaaaat aatcaaagat ttgaatctaa tcattccaga
     1921 tgggaaaaca ttagctatcg taggtccttc cggtggcggt aaaaccacgc ttttacgtat
     1981 cttggctggc ttagaaagtt ctgacagcgg agaattttat ttagacggtg taccgttcaa
     2041 cccattcgag cttaaaatga aagaacaagt tgtaggagtc gtattccaag acttccagct
     2101 atttccacat ctgaatattt ttgaaaacat taccttagca ccaaaaatgg tattgaaaca
     2161 gacaaaagaa gaatacacca aaaaagcaga agaattagcg gcttcattag ggattagtga
     2221 tttattgaat atctatcctt atcagctttc tggcggacaa aaacagcggg tagcgattgc
     2281 cagagcattg gctatgaatc cacgggtatt ggcttatgat gaaccaacaa gtgctttaga
     2341 tcctgaatta cgccagcaag tcgagcgttt gatcctatca ttgaaagaag atggggtaac
     2401 ccaaattatt gtcacccatg atttagtatt tgcagaaaat gtcggtgatc aattactaaa
     2461 agtttcccca attcattaag aaaggagaag atagagatga aaaaaaccag ttttctattc
     2521 gctttgatcg caggtcttct tctttttgca ggctgttcga acaaaaaaac ttcagcagat
     2581 aattgggcga ctatcaacga agagaaaaaa atcgttatag gtttagacga tacatttgtt
     2641 cctatgggat ttagagataa agacgggaag ctcacaggat ttgatattga tttagccaaa
     2701 gcagtctttg aacaatatgg gattacggta gactttcagc ccattgactg gtcaatgaag
     2761 gaatttgaac tgaacaatgg aacgatcgat ttgatatgga acggctattc caaaacgccg
     2821 gcaagagaaa agaaagtgca atttacaaaa ccttatatgg aaaacgatca agtgctgatc
     2881 acacctaaag aatcaggaat cacaaacttt ggacaaatga ataataagag attaggtgcg
     2941 caaaatgggt catctggtta tgatgtattt acgaaacaac cagaagtatt aaaagagata
     3001 gtcaaaaacc aagatgctgt tttgtatgat agttttaatg aagcattgat cgatctgaaa
     3061 agcagacggg tcgatggtct gctgatggat aaagtatacg cagactatta tttgaaacaa
     3121 agaaacgaac tagaagactt caacataaca aaaggttctt atgagagtga ggattttgca
     3181 gttggtgcac gcaagtcaga tagtacatta gtgaaaaaaa tcaatgaagc cctagatcaa
     3241 ctagagaaaa caggtgaatt ccaaaaaata tccgataaat ggtttggcga agatgttacg
     3301 ccgagaaatt aacaaatagg ggactgtgac aaaagacgtg tcacagtccc tttttccgaa
     3361 taaacagtgt tctcctgcca actccttgtg actcggagct gaatggcagg ctcacaacct
     3421 cttggtaaac ggtgttcaaa agccagtttt tcggtattaa gccaaattta tgcaaaaatg
     3481 tagaaagcca ttttcacaaa atttttctta ctactcgaaa ctaagcgact ttttcacaac
     3541 ctcctttaca taattttatt ttgttgtgga aggggtttcg tgttattcta aaagaggaac
     3601 gattgaaggt tataggaaag gaagagatga atggaacaca aagaacttac ctcatttcca
     3661 aaagattttc tgtggggctc tgcttctgca gcgtatcaag tagaaggagc ttggcaagaa
     3721 gatgggaaag gagaatcagt ctgggatcga tttgtacgga tacctggaaa gacatttaaa
     3781 ggaacgaacg gtgatttggc agtagaccac tatcatcgtt ataaagaaga tattgcttta
     3841 atgaaagaac aaggtctgaa agcttaccgc ttctctgtag cttggacacg gatttttcct
     3901 aatggacgag gagaagtcaa tcaagctggg ctcgcttttt atgagagatt gatcgacgaa
     3961 ttgattgaaa acgatattga acctatgttg actttatatc attgggatct tccacaagca
     4021 ttgcaagatg aatataatgg ttgggaatca cgtcagatca ttgaagattt cactcattat
     4081 gcagaaacat tatttgaggc attccgcggg aaagtccatt actggattag tttgaatgaa
     4141 caaaatatct tcacttcatt aggttatttg ttagcagcac atccgccagg tgtgacagac
     4201 ccgaaacgaa tgtatgaagt taaccatatt gctaatttag caaatgcatc tgtcatcaat
     4261 aaatttcacg agttagaaat gcctggaaaa atcggaccaa gctttgcgta tacgccaaat
     4321 tatccaattg acagtaatcc tgaaaatgtt ctggctgctg aaaatgcaga agacctgatg
     4381 gccaattatt ggatggatgt ctatatgtgg ggaaaatatc cgattgctgc catgaggttc
     4441 ttagaagaaa aaggatgggc accaacgatt gaagctggcg atgcagaatt gctagaatct
     4501 gctaaaccag attttcttgg gatcaattac tatcaaacgg cgacgaatgc attcaatcca
     4561 ttaaacggag ttggtgctgg aaaaatgaat acgacaggaa aaaaaggttc atcagaagaa
     4621 actggagttc ctggaatgta taaaaaagtt gagaatccat ttgtcgaacg aacaaattgg
     4681 gactgggaga ttgacccaga aggattaagg atcggactgc gtcgtatcac aagccgttac
     4741 cgcatgccgg tactgattac ggaaaatggc ttaggagagt atgacaagtt gacagaagat
     4801 aaacaaatcc atgacgacta tcgaatcaat tacttacaaa gccacgtcaa agccatcaaa
     4861 gaagcaattt cggatggagc agaagtccta ggctattgca cctggtctta tacagatttg
     4921 cttagctggc tgaacggtta tcaaaagcga tatggatttg tatatgttga tcaagacgaa
     4981 acacaagaag gatctttgga acgatataag aaagacagtt attactggta ccaaaaactg
     5041 attgaagaaa atggagagaa cctttagttc acatacttaa aacaagaagg tacggctgga
     5101 tatggccgtg ccttttcttg ataaaatcat ttagaaatta agaaaaactg taaaataagc
     5161 tgaaaaatga aagaaagcgt cagttctttc aaacaaaact ttagttccct tacttttttt
     5221 tgtataatga aacttgatga aatgagggat aataaatggc acaaaagaaa cggcctacta
     5281 aaaaaaggaa aaagacgaaa aaaacgaaaa agcaaaaaca gcagcaggaa caaatgctga
     5341 cgattggtgt tgggtttgcc ttgattttat ttgctgtctt cggcttttta aaattaggtt
     5401 ttttaggaat attgattgca aacggctttc ggatcattgc aggaaatact taccaaatcc
     5461 tctgtctttt attagccgta ttaggatttt ggattgtcat taagaataca gagttttcaa
     5521 ttggtaaaaa ccgacgctgg tttggcggca tactctttta tttcggtatc ttattattgt
     5581 tacatgccca tttatttggc aaactccata ctggggaacc aaatatcatg gggacgacat
     5641 gggatttgtt agccagtgat ataaaacaaa gtcaagtaga caataacgtt ggtggtggga
     5701 tgattggagc aatcctttat cattttactt acttcttgat tgcacagcct ggaagttatt
     5761 tagcagcagt gctattactg gcaggaggag cattcctgtt cagtaatctg gaaggctatc
     5821 agctgttaaa tggtatccaa tcaattggag aacgagtaca ggaattattg gaaggtgatc
     5881 ctgccaagca agctcgaaag caagcggcaa aggaagagag aatgaaacag cgcgcagaag
     5941 cgaaagaagc aaggcgctta gctgcaaaag aagctgcaga aaaagaagct gcagaatatg
     6001 agaagaaaaa agcggtgagc cagcaaaggg aaaatactgc tgatgaatgg caggaaccag
     6061 aaaatacaga gccggagcag ctaagttttg ttccaatcga cagttttcaa gaaaatatcc
     6121 atcctgcgaa tctagagaaa ccagtaccag atacaccaaa acagaccaat acagcagaag
     6181 gtttttcaga tgagttgcca gaggatgacg gtacctcatt agaatttgag attgaagcag
     6241 aacaggaaaa tcaagactat gaattaccga cagttgattt gcttgattct ataccaactg
     6301 tggatcaaag tgatgagtat aaaaaaattg aaaaaaatat cggcgtatta gaacaaactt
     6361 tccaaagttt cggtgtcgat gccaaggtcg tcaaagccag cttaggacca tccgtcacaa
     6421 aattcgaagt ccagccggct gtaggggtaa aagtaagtaa aattgtgaac ttaacagatg
     6481 atatcgcgct tgctttagcg gcgaaagatg tccggatgga agcaccgatt ccaggtaaat
     6541 cactgattgg tattgaagta ccaaatggaa agatcagtat ggtttccttt agagaaatca
     6601 tcgaagcaca accaaaccac ccagataaat tactagaagt acctttagga cgtgatgttt
     6661 ctggacgggt acaaacggct gatctttcga agatgcctca cttactcgtg gctggttcga
     6721 ctggtagtgg gaagtcagta gcaattaatg ggattatcac aagtatatta atgcgtgcta
     6781 agccccatga agtaaaactg atgatgatcg atccgaaaat ggttgaattg aatatgtaca
     6841 acggcatacc tcatctgttg acgccggtcg tgacgaaccc tagaaaagca gcgcaagcct
     6901 tacaaaaagt agtacaagaa atggaagaac gatatgaaaa attcgcggca accggagtaa
     6961 gaaatatttc tggttacaat gaatttgttc aacagaaaaa cttggaaaac ggaacgaaac
     7021 atccaacctt accattcatc gtagtgatcg tagatgaatt agctgatttg atgatggtag
     7081 ccagcaatga agttgaagat gcgattattc gtttagcgca aatggctcgg gcagcgggga
     7141 ttcatatgat cctagctact caacgtccaa gtgtggatgt tatcacagga atcatcaaag
     7201 caaatgtacc ttccagaatg gcatttgcgg tatcaagcgg tacagattct cggacgatca
     7261 ttgattctaa tggggcagaa aagctcttag ggcgaggaga tatgctattc ttgccaatgg
     7321 gtgagaataa accgatccgt gtgcaaggag cattcatttc tgaccacgaa gtagaacggg
     7381 tcgttcaatt tgttacagat cagcaagaag ctcattatga agagaaaatg atgccgacag
     7441 atgaagttga aacgacaggt gctccagaac agccacaaga tgagctgttt gaagaagcaa
     7501 aagcacttgt cgttgaaatg caaacggcaa gtatttccct tctccaaaga agatttcgga
     7561 tcggttacaa ccgagcagca cgtttagtag atgaactgga agctcatgga gttgttggcc
     7621 cgtcagaagg aagcaagcct cggaaagtat tcattgagca ggaagaaaca tcagaaacgt
     7681 tgccggacga taatgacatg taaaacttgt gtgatgaagg gttgaaaaac gaataaccaa
     7741 taaaaagaca aggcatgatg ataccaaaag aaaaaatggt ttttttccat cttttctaat
     7801 acggtatgat gtcttgtctt tttagttttt gtgagattaa agttctattc gatttatttg
     7861 tgaagtattg cattaaggaa atgaatcatg tatagtatgt agcgaaactt tgagaaaaag
     7921 agggaaaata ctttggaaaa acagtccatt tatggcttga cgaatgaaga attaatcaat
     7981 tggtttatag aaaacggcga gaaaaaattc cgagctgccc aagtttggga atggttgtat
     8041 caaaaacgtg taagtaattt cacagaaatg acgaatctat cgaaacaatt gatcgaaaaa
     8101 ttatcggagc attttatcat caatcctcta aaacagatgg tcgtccaaga agcttcggat
     8161 ggtacagtaa aatatttgtt tgaactccct gataaaaaca tgattgagac tgtactgatg
     8221 agacaagaat atggcttgtc agtttgtgtg acgacacaag taggatgtaa tatcggctgt
     8281 acgttttgtg caagcgggct gctcaaaaag aaccgtgatc tgactgctgg agaaattgtt
     8341 gcacagatca tgatggtcca acattatttt gatgagcgca atcttggcga acgcgtttca
     8401 catgtcgttg taatggggat cggtgagcca tttgataatt acgacaatgt aatggacttt
     8461 ttgcatatca tcaatgatgc caaaggattg gcaattggtg ctcggcacat tacagtttcc
     8521 actagtggat tagcacacaa aatcaaggaa ttcgctaata acggcttgca agtcaatctg
     8581 gctatttcct tgcacgcgcc gaataatgag gtgcgaacat cgatcatgcg gatcaaccga
     8641 agtttcccaa ttgagaaatt gatggaagct gtggatgagt atttagagaa aacaaaccgt
     8701 cgaatcacgt ttgaatatat catgttgaat caagtaaatg atagaccaga gcatgcgcaa
     8761 caattagcag atctattaaa agataagaaa aaattagcat atgtcaactt gattccatat
     8821 aatccagtca gtgaacatga tcaatattct cgcagtccaa aagctgatgt attgagattt
     8881 tatgatgtat tgaagaaaaa cggcgtaaac tgtgtgatac gtaaagaaca tggtacagat
     8941 attgatgcag cttgcggaca attgcgaagc aagcaaatga aaaaaacagt tgccaaataa
     9001 tcgtatccta ctcgtttttt caattaccat aaaaggagca gttttcttaa ttaaggaact
     9061 gttcaactta aataagagaa aagattctat agcgaaagct ataaagcttt ttttgacaag
     9121 aaaaaccctc atcaagcaaa gaaatgtcct caatttgatg acagcctgct tgatgggggt
     9181 tttagcaatt atttaactga ttttcttata tccttgctgg ctttcttgtg tagcatgata
     9241 agtttctacg tcaagaatgg cctgtttaaa tttttctcgg gtgatttcat aaggcacatg
     9301 tttaatatca tttgttgcca tggctttatc taagattgct tctaaatcgg tcgtgacatt
     9361 gagattcatg tcggttaacg atactggaag ttggttcttt ttcataaaca catagatttt
     9421 atcaaacaaa tcgaactgct gatccatcat caataaggtc agtacaccat aactgacaga
     9481 tgctccgtga aggtatccct ccattttttc tagaacagtc gtaccgtaat agaatgagtg
     9541 tgccatagca gaattatagt tattgttaac taaaacagaa accaatccgg tagtaacaat
     9601 gatatccgaa acgacttgtt caattgcata tgagggacga tcgtcggcac tatctttcaa
     9661 tgcttgcgta ccataagtca agagacgatc aatgcacatt tttccgattt ggattcctaa
     9721 tctatccgtg tagcctagat catcgccacg agcagaaaaa gtagattcga cttctttgga
     9781 taaagcatcg ccaatacctg cccataagta tttatcaggt gcttcggcaa taattgctgt
     9841 atcaatgaag gtgtgtacag gtggttttga aggaaagaag agtccgtcca ttacgccttc
     9901 ttcgttatag atgacacata ccgcagtgac gggcgaacag ttcgaaccaa tagtgggaaa
     9961 ggtgaagact ggtttagcta gtttttcacc aatgactttt acagtatcgc aagcacggcc
    10021 accaccgact gcaaacagga cgtctgcttt ttggacactt tcatgctgga tcaactgttc
    10081 aatatttcca taagttgaat tacccccgta ccagagactg gcgacaacct ctatttcctg
    10141 tgtcaaagct acttttattt tgttttctgc ctttgaaagt gcagtcttcc ctccaatcag
    10201 cactactttt gtcccatatt ctttgacgat tccatttgct ttttcatacg catctgttcc
    10261 aatggtataa tttggtaaga aaacactttg actcatattc atttccttct ttcttcgttg
    10321 aaacgggtta aaattttaaa gagtgatcga tctgactgcg gctgtagatg gagtagaggt
    10381 gaacctaaga caggataagt gatgaataag gtggcatgaa caccttgaaa tcatcaataa
    10441 gtatcctaaa aacgaacaag tacaaaaaca ttgaaaacga caataatgaa tttttgtt
//
LOCUS       DACDKM010000095        10488 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20834_2016
            SAMN12640370-rid9891663.denovo.095, whole genome shotgun sequence.
ACCESSION   DACDKM010000095 DACDKM010000000
VERSION     DACDKM010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640370
            Sequence Read Archive: SRR10040891
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10488)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10488)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742835.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742835.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:04:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,012
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 29 of 75
            Pseudo Genes (incomplete)         :: 33 of 75
            Pseudo Genes (internal stop)      :: 19 of 75
            Pseudo Genes (multiple problems)  :: 5 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10488
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640370-rid9891663.denovo.095"
                     /isolate="E20834_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(<1..751)
                     /locus_tag="IV334_002215"
     CDS             complement(<1..751)
                     /locus_tag="IV334_002215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HAP8944268.1"
                     /translation="MEEGIVPTQPQRSSKKLFGVVVLNAVITLSEFIGGIMSGSLSLV
                     SDAFHNLSDTLAIIFSYGAQKMARKEANKKRTYGYQRLEILSAFINSFILIILSLFLT
                     VEAFKRFTSPEKINSHLMLTVAVIGLLANLFSVLLLRQEADESLNIKSSYLHLLSDTL
                     SSVSVIIGAILIRFFDIYWIDPVITLIISIYILIEAIMVIKKAAAILIQSAPAIDYEK
                     MEQEIEAIDGVKDVHHVHIWQYSEKIIIFDGH"
     gene            897..1700
                     /locus_tag="IV334_002216"
     CDS             897..1700
                     /locus_tag="IV334_002216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1189 domain-containing protein"
                     /protein_id="HAP8944269.1"
                     /translation="MTTKQLIISSFIRFKDLKEARNVPFWKAIVYLLVLSIIMALPIS
                     FQVFQVLNNIQLDGQKIATRIPDFTIQNGQLQTEEKEGFIYQTNSIIFTFDPEGKRTE
                     QDISTDLMGNFVSVGMLKNKLIIALPNTGTTSALLNNNQLELPYTNESLKNLTGKQLR
                     SFLSEASIPLWIKVLTFIFSIYPSFLNLLITLLFTNIAAFLYARFRLTKATFLDCLKT
                     LIYSISLPTIIATILMVFLPSFDTSAFIAFAGIFIFAQAVKGWAKISIN"
     gene            1878..2825
                     /locus_tag="IV334_002217"
     CDS             1878..2825
                     /locus_tag="IV334_002217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacid dehydrogenase"
                     /protein_id="HAP8944270.1"
                     /translation="MSERIIYADRPFRDEFIEEIHTIAPGYIFKTELQPEDLQNVEIS
                     LGWNSDYQEKLLASSTLKWVQSISAGVDQLPLETFSKKHILLSNGSGIHSESITEHVL
                     GIILGYSRGLFQAQKAQSDMKWLGSSVHYQSIQGKKILIIGTGHIGKMIAKKCKALDM
                     NCYGINTSGHPAEGMEKTFSLNQLKEVLPEVDFVVNILPLTEETTGLFDQHMFEHFDP
                     KSVFINVGRGASVKTQDLVQALNNQQLSFAALDVFEEEPLPQDHPLWKMDNVLITSHI
                     AGLTPDFQKKLMAIFLTNLKSYFATHELQTNQVKLSAGY"
     gene            complement(2893..3186)
                     /locus_tag="IV334_002218"
     CDS             complement(2893..3186)
                     /locus_tag="IV334_002218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis protein"
                     /protein_id="HAP8944271.1"
                     /translation="MKLTVTPKAQKWFDSEIDLPETYGIRFFGKVYGKTEVHDGFSIG
                     MSVEQPEHPVKKVEMDGTLFFIEEADEWFFKGYDLLVDYDPNLEEPTYHFKKQ"
     gene            complement(3214..3879)
                     /locus_tag="IV334_002219"
     CDS             complement(3214..3879)
                     /locus_tag="IV334_002219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HAP8944272.1"
                     /translation="MKQNFAIVGLGRFGGSICRTLIEAGQEVLAIDSNEDRVNEYMNI
                     ATHAVVANAQDEMTLRSLGIRNFDHVIVAIGEDIQASILVTLMVKEMGVPNILAKAQN
                     EYHARVLEKIGADRVVHPERDMGIRIAHNLVSKNILDYLELSDKYSLAEIRVSNPKFF
                     NKTLAELNFRQRFDLTVVAIRRVDQTVIASPAADEYVRENDNLLVIGETGDVDILDDK
                     MNQ"
     gene            complement(4019..4651)
                     /locus_tag="IV334_002220"
     CDS             complement(4019..4651)
                     /locus_tag="IV334_002220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP8944273.1"
                     /translation="MIKVLLVDDHEMVRLGVSSYLSIQEDIEVIGEAENGRQGYEKAM
                     TLRPDVILMDLVMEEMDGIESTKAILKDWPEAKIIIVTSFIDDEKVYPAIEAGAAGYL
                     LKTSTAHEIADAIRATQRGERVLEPEVTTKMMEKMSRRNEPVLHEELTNRENEILMLI
                     SEGKSNQEIADELFITLKTVKTHVSNILAKLEVEDRTQAAIYAFKHGLVK"
     gene            complement(4658..5725)
                     /locus_tag="IV334_002221"
     CDS             complement(4658..5725)
                     /locus_tag="IV334_002221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HAP8944274.1"
                     /translation="MMGKISRAMLAVYSGIAAFLIILFSLFTYFYASNQSHWWGELLR
                     ARLLYVPLIFHLLAISLGVGLIVFLLLSLIQKTKYGKIEEKLRALSSGNYESKLLLLP
                     IPSASDDLYIKDIDKEITKIKEKMIEVSSELQIVTSRPQYVDGQTKEEILELERHRLA
                     RELHDSVSQQLFAAMMMMSALTEQAEKSETPEMFRKQLKMVAEIINASQSEMRALLLH
                     LRPVNLEEKSLKQGIEQLLKELQNKIQISLKWDVEDVKLSSSIEDHLFRIVQELLSNT
                     LRHAKANELEVYLHKIDNNLLLRIIDDGTGFNMNETKTGSYGLNNIKERVAGIGGTVK
                     IISFKGQGTSVEIKVPLMKEA"
     gene            complement(5722..6453)
                     /locus_tag="IV334_002222"
     CDS             complement(5722..6453)
                     /locus_tag="IV334_002222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-active antibiotics response protein"
                     /protein_id="HAP8944275.1"
                     /translation="MNSSWRFFVVVEALLLLFAVWQIVNNTGLLLLVIFGIFNIYLAM
                     RKHPRTKFQNFQLILGSLVIFFSLVNSPALWMMAVFAVLFIGLKGVEISGIDLTKNAF
                     WRKKQIMMIQTEQLKTHNNERKRQQLFGNQRIGNDVYEWDDINIAVISGDTIIDLGNT
                     LLPKDDNIVIVRKGIGRTRILVPLGVAIRLEHATLVGNVLFEEEQFSLKNEQIKIYSS
                     DYDENPRRLKIITNTLIGDVEVIRI"
     gene            complement(6622..7101)
                     /gene="greA"
                     /locus_tag="IV334_002223"
     CDS             complement(6622..7101)
                     /gene="greA"
                     /locus_tag="IV334_002223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HAP8944276.1"
                     /translation="MVEKVYPMTLEGKEKLEKELEELKTVKRGEIIERIKIARGFGDL
                     SENSEYESAKDEQAFVEGRITTIENMIRFAQIIDNDGVDADEVSIGKTVTFIELPDGE
                     EEEYTIVGSAEADPFSGKISNDSPIAQALIGKRKNDEVTIATPGGDMLVKIVKVEQA"
     gene            complement(7237..8412)
                     /gene="mltG"
                     /locus_tag="IV334_002224"
     CDS             complement(7237..8412)
                     /gene="mltG"
                     /locus_tag="IV334_002224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HAP8944277.1"
                     /translation="MSGNKQHPSKDKKEASKAATADIIDKRRNLRKKEDKIVRKIILV
                     IALTLLIIGGFLGFTVYRYVDSGLKPLDKSDDQLVQVEIPSGSSNKQIGEILEKDNII
                     KSGIVFNYYTKFKNLTGFQAGYYQLAPNMTLDEIGKQLQEGGTSEPTKVADGKIAIPE
                     GYDIDQIAERVAKVTGKDKKEFLDLVNDETFFNELHQKFPELLESASKAENTKYRLEG
                     YLFPATYDYYTSTSLKDLVIEMVNKTNTVMQNYYSAIKQKNLTVQEVLTLASLVEKEG
                     VKENDRKNIAQVFFNRIKANMPLQSDISVLYALGEHKELVTYEDTAVDSPYNLYTNTG
                     YGPGPFDNPSEEAIKAVLEPAENDYYYFVADTSTGNVYFAKTYEEHMELVEKYVNNS"
     gene            8616..9785
                     /locus_tag="IV334_002225"
     CDS             8616..9785
                     /locus_tag="IV334_002225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase family protein"
                     /protein_id="HAP8944278.1"
                     /translation="MKKGKHAKKRKYSFLMIVVVLFSLSAFSTGTILGWQFYNNEHSQ
                     IVAHAQSAQKKLILTTNEQQLVEKIDQLLRKNDYIGSIYVKKNNRIIMEKGYGYANKL
                     TQQPNDPSLYYQIGSVQKALTTLLILKQVERGHLSLDTKLAQFYPQIAGSQQITIRDL
                     LYMRSGLHRTASPTIPMTDEEVVQFAIQHLKFTDYQTSHYDSLNFTILTGILIKLTNQ
                     PYEKLLTDDIIEPLGLQHTKFYDQVKNSSNHALSYQMSKEDDYFKPLTESETEIHNEL
                     GTGNISMSVYDLNTLFTQVLSGKLVSKDLLFSLWRENANGHPYSGGVYSGDNYILSQG
                     NIDSFDTSAIFSKDSQDAIIMESNIKADKTIKLPTADLRNQIYDLMEGTTKLANS"
     gene            complement(10052..>10488)
                     /locus_tag="IV334_002226"
     CDS             complement(10052..>10488)
                     /locus_tag="IV334_002226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8944279.1"
                     /translation="TYSNEQMENKLEEFENFDYVPIGYKKDQLLLKNHQVTEDGQVIY
                     SSFSPNKDEITFTFKSSKGKTIVLPVFRYKGLTVTVNSKNATVLKQGGPFVTVKSESG
                     INKVTIRYSYTFFSKISLFISIITLIVLLFYCIKLKIRTSHE"
BASE COUNT         3227 a         2087 c         1626 g         3548 t
ORIGIN      
        1 tatgtccgtc gaaaataatt atcttttcgc tatactgcca aatatggaca tgatgaacat
       61 cctttacccc atcgattgct tcaatttctt gttccatttt ttcatagtcg atagcaggtg
      121 cactttgtat caaaatagcg gcagcttttt tgatcaccat tatcgcttct atcaatatat
      181 atatcgaaat gatcagtgta ataactggat cgatccagta gatatcgaaa aaacggatca
      241 aaattgctcc gataatcaca cttaccgaag acagtgtatc acttaacaaa tgcaaataac
      301 tagattttat attcaaactt tcatctgctt cttgtcgtaa taataatact gagaacaaat
      361 tcgcgagcaa tccaataact gctactgtta gcatcaaatg actatttatc ttttctggtg
      421 aagtgaaccg cttgaatgct tcaacggtta aaaacaagga taatatgatc aatatgaatg
      481 agttgatgaa agcactcaat atttctaaac gctggtagcc atacgtacgt tttttatttg
      541 cttcttttcg ggccattttt tgtgccccgt aagaaaaaat aattgccaat gtatcactaa
      601 ggttatggaa agcatctgaa acaagagaca aactccctga cataatccct ccaataaatt
      661 ctgataaagt gatcaccgca ttcaaaacca ctactccaaa tagttttttt gatgatctct
      721 gtggctgtgt aggcactatt ccttcctcca ttttatttat attggatagt ctttagacaa
      781 gaataacatt tttttgatat ttcaccaatc attcagctgt ttccttaaag cacttgtatt
      841 tcacttgata cttcgctaga attgcaagtg atgacactac tagatgagga gtacttatga
      901 caacaaaaca attaattatc agttccttta tacgattcaa agacctaaag gaggcgagaa
      961 atgtcccatt ttggaaagcc attgtctatc ttttagtttt gagtataatt atggctttgc
     1021 caatctcttt tcaagttttt caagtcttaa acaacattca attggatggg caaaagattg
     1081 ctactcgcat tcctgatttt acaatccaaa acgggcagct tcaaaccgaa gaaaaagaag
     1141 gattcattta ccaaacaaac tccattatct tcacatttga tccagaggga aaacgaacag
     1201 aacaggacat ctctactgat ttgatgggga attttgttag tgtcgggatg ttaaaaaaca
     1261 aacttatcat agctcttcct aatacaggca cgacttctgc tttgttgaac aataaccaat
     1321 tagaacttcc ctacaccaat gaatctttaa aaaacttaac aggtaagcag ctccgttctt
     1381 ttttaagtga agcatctata ccactttgga taaaagtcct gacatttata ttttctattt
     1441 atcctagttt tctgaatctg ctgatcactt tgctatttac caacattgct gcttttctct
     1501 atgcacgctt tcgactaaca aaagctactt ttcttgattg tttaaaaaca ttgatctatt
     1561 caatttcatt gcctactata attgcaacaa tcttgatggt ctttcttcct tcttttgaca
     1621 ctagcgcctt cattgctttt gcaggaatct ttatttttgc tcaagctgtc aaaggatggg
     1681 caaaaatatc gatcaattaa gaaataaaga caactcgtta tttctacttc aatagaccct
     1741 tttagaggtt gtgacatttt tttgtcacag cccctttttt atttgaaaaa tatttctttc
     1801 tcaaaaaaca agcaaggcgc ttcaaagttc ttcttgtatg cgctacactt agagtaaaga
     1861 aaggcgggaa attaactatg tcagaaagaa tcatttatgc agatcgtcca tttcgtgatg
     1921 aatttatcga agaaatccat actatagcgc ctggttatat ctttaaaaca gaattacaac
     1981 cagaggatct acaaaatgta gagatcagtt tgggctggaa ctcagactac caagaaaaac
     2041 tattagcctc ttctaccttg aaatgggtcc aatctatctc agctggtgtt gatcagctac
     2101 cccttgagac tttttcaaag aaacatattt tactttcaaa tggtagtggc atccacagtg
     2161 aatcgattac ggagcatgtc ttaggaatca tccttgggta ttctcgtggt ctttttcaag
     2221 cacaaaaggc acagagtgac atgaaatggt taggttcatc ggtccattac caatctatcc
     2281 aagggaaaaa aatactaatc attggaactg gccatattgg aaaaatgatt gcaaaaaaat
     2341 gcaaagcatt agacatgaac tgctatggaa tcaatacatc cggtcatcca gcagaaggaa
     2401 tggaaaagac tttttcttta aatcaactaa aggaagttct tcctgaagta gatttcgtag
     2461 tcaatatctt acctctaacg gaggaaacaa ctgggctctt tgatcagcat atgtttgaac
     2521 actttgatcc aaaatctgta ttcatcaatg ttggccgtgg tgcttctgtt aagacacaag
     2581 atctggtgca ggctttaaat aaccagcaac tttcctttgc agcattagac gtttttgagg
     2641 aagagcctct tccacaagat catcctcttt ggaaaatgga caatgtactg attacttccc
     2701 acattgcagg tttgacacct gattttcaaa agaaattaat ggctattttt ttaaccaatc
     2761 tgaaaagcta ctttgccacc catgagcttc aaaccaatca agtaaaattg tctgcaggat
     2821 attaatagct gaaaacaata ttttcaaaag aaaaaagaga aggtgacaaa aattgtgtca
     2881 ccctctcttt ttttattgtt ttttaaagtg ataggttggc tcctccaggt tgggatcata
     2941 atcaacaagt aaatcatatc ctttaaagaa ccattcatct gcttcctcga taaaaaaaag
     3001 tgtcccatcc atttccactt tctttacagg atgttctggc tgttcaactg acattccaat
     3061 cgaaaaacca tcatgaacct ctgttttgcc gtagactttt ccaaaaaaac gtattccgta
     3121 agtttctggc agatcaattt ctgagtcaaa ccacttttgt gcttttggcg ttaccgttaa
     3181 tttcatccca acaacctact ttctcttttc tttctattga ttcatcttat cgtctagaat
     3241 atccacatcg cctgtctctc caataaccag tagattatca ttttcacgaa catattcatc
     3301 tgctgcagga gaagcgataa ccgtttgatc tactcttcgt atggcgacta ctgttaagtc
     3361 aaaacgttgc ctgaaattta attcagctaa tgttttgttg aaaaatttag gattggagac
     3421 tcggatttct gctaaagaat atttatctga caactctagg taatctaaaa tattctttga
     3481 cactaaatta tgcgcaatcc ttattcccat gtctcgctcg ggatggacga cgcgatcagc
     3541 tccgatcttt tcaagtactc tggcatgata ctcattttgg gcttttgcta aaatatttgg
     3601 tacgcccatt tcctttacca tcagagtaac taagatactg gcttgaatat cttctccaat
     3661 agcgacaatt acatgatcga aatttcttat gcctaatgat cgaagtgtca tttcatcctg
     3721 tgcatttgcc acaacagcat gagtggctat attcatatat tcattgaccc gatcttcatt
     3781 actatcaata gccagtactt cttgaccagc ttcgatcagt gttcggcaga tacttccgcc
     3841 aaatcgtcca agacctacaa ttgcaaaatt ttgtttcacc gttttcctcc tttagtatct
     3901 tcttttctca gtataacaaa gcctttacaa aaacaaaagc aagcatcatt attgtaaaaa
     3961 agccaagaaa aataatagtt attttctcag cttttgatta tcctctttat catcgttttt
     4021 atttcaccaa accatgtttg aatgcataga tagcggcttg tgtacggtct tccacttcta
     4081 gcttagccaa tatattggaa acatgtgttt ttactgtttt cagtgtaata aacaattcgt
     4141 ccgctatttc ctgattactt tttccttcag aaatcagcat taaaatttca ttttcccgat
     4201 tcgttagttc ttcatgtaat acaggctcat ttcgccgact cattttttcc atcatttttg
     4261 ttgtcacttc tggttctaat actcgctcgc cacgttgcgt tgcccttata gcatcagcaa
     4321 tttcatgtgc ggtagaggtc ttcaacaaat accctgctgc acctgcttca atggcaggat
     4381 aaactttttc gtcatcaata aaactggtta cgataatgat tttagcttct ggccaatctt
     4441 ttaagattgc ttttgttgat tcgataccat ccatttcttc catgactaag tccattaaaa
     4501 tgacgtccgg tcgaagtgtc atcgccttct catagccttg tcgtccattt tcagcttctc
     4561 caattacttc aatgtcttct tgaatagata aataagaaga gacgcctaag cgcaccattt
     4621 catggtcatc tactaataaa acttttatca tccttatcta tgcctccttc atcaaaggga
     4681 ccttgatttc tacacttgta ccttgacctt taaaactaat gatttttact gttccaccaa
     4741 taccagctac tcgttctttg atattgttca atccataact tcccgttttc gtttcattca
     4801 tattaaaccc tgttccgtcg tcaataatac gtaacaaaag attattgtct attttgtgca
     4861 aatatacctc taattcattg gctttagcat gtcttaatgt atttgataac aattcttgaa
     4921 cgattcggaa caagtgatcc tcaatggaac tagatagttt tacatcttct acatcccatt
     4981 tcagcgaaat ctggatttta ttttgcaatt ccttcaataa ctgctcgata ccttgcttta
     5041 aacttttctc ttctaaattg actggacgta gatgaagaag cagcgcacgc atctcagact
     5101 gggaagcgtt gatgatctct gctaccattt tcaactgctt gcgaaacatt tctggtgtct
     5161 cgcttttttc tgcttgctcc gtcaatgccg acatcatcat cattgctgca aacaattgtt
     5221 gcgaaactga atcatgcagc tcacgggcta atcgatgtct ttctaattct aaaatttctt
     5281 ctttggtttg tccatctaca tattggggac ggctcgttac aatttgtaat tcactagata
     5341 cttcaatcat tttttctttt atcttagtga tttccttatc aatatctttg atgtataaat
     5401 cgtccgatgc acttgggatc ggaagaagca acaatttgga ttcataattg ccagaagaaa
     5461 gtgcacgcag cttttcttcg attttcccgt attttgtctt ttgaataagt gataataata
     5521 gaaagacaat taatcctaca cctaaggaaa tggctaacaa atggaagata agcggaacat
     5581 ataataaacg tgcacgcagt aattctcccc accaatgact ttgattgctg gcataaaaat
     5641 aagtgaaaag tgaaaataga ataataagga aagccgcaat ccccgaataa acagctagca
     5701 tcgctctgga tatttttccc atcatattcg tatcacctcg acatctccaa tcaaggtatt
     5761 agtaataatc ttcaatctac gagggttttc atcataatca ctgctgtaga ttttgatttg
     5821 ttcatttttc aaagaaaatt gctcttcttc aaacaagaca tttcctacta atgtcgcatg
     5881 ctctaatcgg atggctacgc ccaaaggtac taagatccgt gttctgccta tcccttttct
     5941 tacaatcaca atattatcat cttttggcaa aagcgtattg cccaaatcta tgatagtatc
     6001 accagaaatg acggcgatgt tgatatcgtc ccattcatag acgtcgtttc caatacgttg
     6061 gttaccgaat aattgctgtc ttttccgctc attattatgt gtttttaatt gctctgtttg
     6121 gatcatcatg atctgtttct ttcgccaaaa agcatttttg gttaaatcta tccctgaaat
     6181 ctctacacct ttcagaccaa taaataaaac ggcaaataca gccatcatcc ataaagcagg
     6241 actgttgact aagctaaaaa aaatgaccaa acttcctaat atcaattgaa aattttgaaa
     6301 tttagtacga ggatgtttgc gcatagctag atatatattg aaaataccga atatcactaa
     6361 aagcaaaagt ccagtattgt ttacgatttg ccaaacagca aataacagca gtagtgcttc
     6421 aactaccaca aaaaaacgcc aagaactatt catcgctctt tctccctcct gacacattct
     6481 catttcatca tacctatttt tttaactgtt ggctatagga ctttagatgt aagatcgttt
     6541 gttgaaatct aaaatacaca aagagaaaaa gatctagagt gtatcatcag taagatgaac
     6601 tctctagatc tttcagtcag actacgcttg ttctactttg acgattttaa ctaacatatc
     6661 gccacctgga gtagcaatag ttacttcatc atttttacgt ttgccaatca atgcttgcgc
     6721 aataggagaa tcattggaaa ttttgccaga aaacggatct gcttcagcgc ttccgacaat
     6781 cgtgtactct tcttcttctc catctggtag ttcgatgaat gtaactgttt taccaattga
     6841 tacctcatct gcatctacac cgtcgttatc gatgatttga gcaaaacgaa tcatattttc
     6901 aattgttgtg attcttcctt caacaaatgc ttgttcatct ttcgctgatt catattcaga
     6961 gttttcggaa agatctccga aaccgcgggc aattttgatt cgttcaatga tttctccgcg
     7021 tttgactgtt tttagttctt ctaattcttt ttctaatttt tctttacctt ctagagtcat
     7081 tggataaact ttctctacca tgtgtaacgt ctcctttttt cgataaaata ccgcaaaagg
     7141 taatcttttg aggaatatag tccaaaaaag attaccacgc ttttcttcaa atgtaaatgt
     7201 ttttctaaaa atgatcaatg tctagagcct gtttttctaa gaattattta catatttttc
     7261 cactaattcc atatgttctt cataagtttt agcgaaataa acatttccag tgctcgtatc
     7321 tgctacgaaa taataatagt cattttctgc tggttctaat actgccttga ttgcctcttc
     7381 gcttggattg tcaaaaggtc caggtccata gccggtattg gtatacaaat tatatggtga
     7441 atctacagct gtatcttcat acgtcactaa ttctttgtgt tcacctaatg cgtataaaac
     7501 agagatatct gactgaagtg gcatatttgc tttgattcgg ttaaagaaga cttgtgcaat
     7561 gtttttacga tcattttctt ttacgccttc tttttcaact agagaagcta gagttaaaac
     7621 ttcttgtaca gtcaaatttt tctgcttgat agctgaataa tagttttgca tgaccgtatt
     7681 cgtcttgttc accatttcaa tcactagatc cttaagcgaa gtactcgtgt aataatcata
     7741 tgtggctggg aataagtaac cttccaaacg gtattttgtg ttctcagctt tagatgcact
     7801 ttccaacagt tctgggaatt tttggtgcaa ctcgttaaag aaagtctcat cattcactaa
     7861 atctaaaaat tctttcttgt ctttaccagt taccttcgct acccgttcag caatctgatc
     7921 gatatcgtac ccttctggaa tggcaatttt cccatctgca acttttgttg gctcagaagt
     7981 accgccttct tgcaactgtt tgccaatctc gtcaagagtc atattaggcg ctaactgata
     8041 atagccagct tgaaagccag ttaaattttt aaattttgta taataattga aaacaatccc
     8101 gcttttaata atattgtcct tctccaaaat ctcaccaatc tgcttgttag aagagccact
     8161 cgggatctcg acttggacta attggtcatc tgatttatct aaaggcttca acccgctatc
     8221 tacataacga tacacggtaa atccaagaaa tccgccaata atcaataatg taagagcaat
     8281 cactaaaatg atcttcctca caatcttatc ttctttttta cgcagatttc tccgcttgtc
     8341 gattatatct gctgtagctg ccttactggc ttctttttta tctttacttg ggtgttgttt
     8401 gtttcctgac aaaacaactc ctccttacta tcaagttcgt aactttattc attatacatg
     8461 atatttgaat tggcgtgaat gattttattt aaatattcta tcaaaaatgt aagtagtaaa
     8521 tccattacta taagattaaa ataaatgtta tcaagataca agctatttta gctttattct
     8581 ttctgtaaaa aagaaaggag actaaataaa ctattatgaa aaaaggaaaa catgctaaaa
     8641 aaagaaaata ttcattttta atgatcgttg tcgtgttgtt tagccttagt gcgttctcta
     8701 caggaactat tcttggatgg caattttaca ataatgaaca ctctcagata gttgcccatg
     8761 ctcaatcagc tcaaaaaaag ttgatactga ctaccaatga acaacagctc gttgaaaaaa
     8821 tcgatcaact attaagaaaa aatgattata ttggctcaat ttacgtaaag aaaaataatc
     8881 gcatcatcat ggaaaaaggc tatggctatg cgaataaact cacgcaacaa ccaaatgatc
     8941 cttctcttta ttatcagatt ggttcggttc aaaaagcatt gactactctt ttgattttaa
     9001 aacaagtcga gcgtgggcac ctttctttag atactaagct ggctcaattc tatccacaaa
     9061 ttgctggcag ccagcaaatt actatccgtg acttgctgta tatgcgctca ggcttgcacc
     9121 gaactgcttc tccaaccata ccgatgactg atgaagaagt cgttcaattt gcgattcagc
     9181 acttaaaatt tacagattat cagacctctc attatgattc attgaacttt accatattga
     9241 ctggaatttt gatcaaatta acaaatcaac cgtatgaaaa attattgacc gatgatatca
     9301 tcgaaccttt aggattacag catacaaaat tttatgacca agtcaaaaat tcatccaatc
     9361 atgcactatc ctatcaaatg tctaaagagg atgattactt taaaccactg acagaatctg
     9421 aaacagagat ccataacgaa ttagggactg gtaatatcag tatgtccgtc tatgatttaa
     9481 atacgctgtt tacacaagtt ttatctggaa aactagtttc caaagactta ctcttctcat
     9541 tatggcgtga aaatgcgaat ggacatccat atagtggtgg ggtatatagc ggagataatt
     9601 atatcctgtc gcaaggaaat atcgactctt ttgatacatc tgctatattt agcaaagatt
     9661 cacaggatgc tatcatcatg gaaagcaata taaaagctga caaaactatc aaactgccta
     9721 ctgctgatct aagaaaccaa atctatgacc tgatggaagg aactactaag ttggcaaata
     9781 gctgatatga tgaaaagata gcaaatacta cttctttgaa tagcatttgc ttaaaaaaag
     9841 tactcgacta aacgtaggaa agagtgtggg acaaatctaa aaatcaattt ttgctacaga
     9901 ctttaattcc aaataaacag aacagaaaga ggtgttcttc tttctgttct gtccaaatta
     9961 ttctaagatt tctctttacc aacattattt gacaaagggc ctattaaatc taaaatagta
    10021 tctttagatt gctaaaatat gtgctagcaa atcattcatg gcttgttctt atttttaatt
    10081 ttatgcaata aaaaagaaga acaattagcg tgattataga aataaataat gagattttag
    10141 aaaaaaatgt gtatgaatac ctgattgtta ctttatttat tccactttca ctttttactg
    10201 taacaaaagg acctccttgt tttagtactg tagcattttt tgaattaaca gtaaccgtta
    10261 atcctttata ccggaataca ggtaaaacta ttgttttgcc ttttgaagat ttaaacgtaa
    10321 atgtaatttc atctttattt ggagaaaagc tactataaat aacttgacca tcttctgtaa
    10381 cttgatggtt tttcaaaagc aattgatctt ttttatagcc tataggtaca taatcaaaat
    10441 tctcaaattc ttcaagttta ttttccattt gttcattaga ataagttc
//
LOCUS       DACDKM010000096        10409 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20834_2016
            SAMN12640370-rid9891663.denovo.096, whole genome shotgun sequence.
ACCESSION   DACDKM010000096 DACDKM010000000
VERSION     DACDKM010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640370
            Sequence Read Archive: SRR10040891
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10409)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10409)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742835.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742835.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:04:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,012
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 29 of 75
            Pseudo Genes (incomplete)         :: 33 of 75
            Pseudo Genes (internal stop)      :: 19 of 75
            Pseudo Genes (multiple problems)  :: 5 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10409
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640370-rid9891663.denovo.096"
                     /isolate="E20834_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(25..144)
                     /locus_tag="IV334_002227"
     CDS             complement(25..144)
                     /locus_tag="IV334_002227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3789 domain-containing protein"
                     /protein_id="HAP8944280.1"
                     /translation="MEIVKDILLVFGGSMLGVTVMCLMHASAAADRAMEKKGK"
     gene            complement(159..1343)
                     /locus_tag="IV334_002228"
     CDS             complement(159..1343)
                     /locus_tag="IV334_002228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HAP8944281.1"
                     /translation="MAQRNLDYRLLKDRRNEYGVSQNKLATTCGLSRPYLNQIENGGV
                     TASTKTMRKIFDQLESFNPELPLTLLFDYVRIRFPTTDARKIIQEILHLKFDYMLHED
                     YAFYSYQEQYVMGDIVVMLSHEEDKGVLLELKGRGCRQFETFLLAQKRSWYDFFEDCL
                     KAGGVMKRLDLAINDRVGLLDIPDLTKKCQKEECISLFRTFKSYRSGELLKPDDKDGM
                     GNTLYIGSLKSEVYFCLYEKDYEQYIKLGIPLDQTETKNRFEIRLKNDRAYHAIQDLL
                     KGRSIESTTFSIINRYLRFADKVEGKRRTNWPLNEQWGRFIGRNQKEIQLTSEPKPYT
                     IERTLNWLGRQVAPTWKMAKELDRLNQTTYIQDMVNNTRLSDRHKKILEQQSMAIENL
                     II"
     gene            complement(1599..1853)
                     /locus_tag="IV334_002229"
     CDS             complement(1599..1853)
                     /locus_tag="IV334_002229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8944282.1"
                     /translation="MIGKIQSDPELEEELYRLNFDIRKNGVKVPDDPYVIDEETDARM
                     ELNQVISELERIADFAKEPDIRQYLFEIKAELEIEGITAE"
     gene            complement(1878..3020)
                     /locus_tag="IV334_002230"
     CDS             complement(1878..3020)
                     /locus_tag="IV334_002230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016613853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="HAP8944283.1"
                     /translation="MTFLDLFAGIGGFCLGMEQAGHQCIGFCEIDDFARQSYKAIHDT
                     SKEVEMHDITSVSDEFIQSLGPVDILCGGFPCQAFSIAGKRQGFSDTRGTLFFEIARF
                     AAILQPKFLFLENVRGLLNHEGGATFETILRTLDGLGYDVEWQVLNSKAYVPQNRERI
                     FLIGHSRDACTEQVFPIIGSSPTSDQNIRNLLNINPSNRGMGGQVYGSDDVAPTLTGD
                     EGIKIALPVDDGISVAGMLLGNFEQGNRVYEITGTAPTLSTKQGGTKIMLRKNETEYQ
                     EVKPGNSVNLAFPNSTSRRGRLGKQSVHTLLTGDQQAVVTDQYQIRKLTPRECWRLQG
                     FPDWAFDRASQVNSDSQLYKQAGNSVTVPVIFDIARRLKEVKTDDL"
     gene            complement(3080..4432)
                     /locus_tag="IV334_002231"
     CDS             complement(3080..4432)
                     /locus_tag="IV334_002231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016613852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF87 domain-containing protein"
                     /protein_id="HAP8944284.1"
                     /translation="MYKGHRIRAGDQHLVYHFVLGWLLALFIGWMSVFYFQELRQFDI
                     SKLSLSTIEIVRSIKDLIYLLGSLVLSGSTMLLYIHFFQDHWRSLWHRQKLARMILEN
                     HWYEVKQTQSEGFFKDLNSSRTKETISYFPKIYYRMKDGLLSIRVQISLGKYQDQLLK
                     LEKKLESGLYCELVEKELKDSYVEYTLLYDIIANRIGIDEVVAESGALRLMKNQVWAY
                     DSLPHMLIAGGTGGGKTYFLLTIIEALLKSDAELFVLDPKNADLADLGTVMPHVYSQK
                     EEISACVEDFYERMMTRSKAMKEMPNYKTGENYAFLGLPPNFLIFDEYVVYMEMLTTK
                     ESAVILNKLKQIVMLGRQSGFFLILACQRPDAKYLGDGIRDQFNFRVALGRMSELGYS
                     MMFGEVDKKFFMKRIKGRGYVDTGGSVISEFYTPLVPKGYDFLREISDIGGLNVNTEG
                     "
     gene            complement(4503..4847)
                     /locus_tag="IV334_002232"
     CDS             complement(4503..4847)
                     /locus_tag="IV334_002232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="damage-inducible protein J"
                     /protein_id="HAP8944285.1"
                     /translation="MEINVEHILDCLDQYGKGELTEEQLTKALTYDEKMFLIMHQGLL
                     EVGNDAEEDFDVLNWLGEEESFFMVIEVNESLCREAETVLEEIGVEMPDAIEVFLKQL
                     VETKQLPIAVND"
     gene            complement(4857..5231)
                     /locus_tag="IV334_002233"
     CDS             complement(4857..5231)
                     /locus_tag="IV334_002233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HAP8944286.1"
                     /translation="MRLTEGIVVDSGLTFGKLRFSALRREVRKQNEDGTVSNEVKERT
                     YNLKSSAQGRMIQVSIPANVPLREFAYDAEVELVNPIMDTVANYVFREGTTVNWFIKA
                     DDLVLKRQPDQGNSTNQNEGKK"
     gene            complement(5244..5558)
                     /locus_tag="IV334_002234"
     CDS             complement(5244..5558)
                     /locus_tag="IV334_002234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HAP8944287.1"
                     /translation="MELKFVVPDMAETFGKIRYAGEGEVLTEGYGRNTTVIGRSYHLY
                     SSKQRADDIEVVVAAEAGEKDFDQDQPLKAVNPHLVAKGYEIENRGFTDYVLYVDDLV
                     KA"
     gene            complement(5672..8897)
                     /gene="fss3"
                     /locus_tag="IV334_002235"
                     /pseudo
     CDS             complement(5672..8897)
                     /gene="fss3"
                     /locus_tag="IV334_002235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286924.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fibrinogen-binding MSCRAMM adhesin Fss3"
     gene            complement(8976..9638)
                     /locus_tag="IV334_002236"
     CDS             complement(8976..9638)
                     /locus_tag="IV334_002236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010740290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8944288.1"
                     /translation="MKQNDGRIIWKNQSELKLILTINEFIEKHGITSSRQYQKKLSEN
                     PNSAPSMWFINKKYGSWENLLISIGRENTGYGKWARMSEQELLEIVEAFIKCEKITSQ
                     RMYEQKSVGKNIPSLSTIKKMLGDIRPLFKEKNDGSRFTDFELLLELKNEIIRLKLQD
                     DLSMTKFRKLVQSPKLPSVDTIMKRTNKNWEELMAEIGFDYRRIKIYKQRNNLSKTKK
                     TK"
BASE COUNT         2847 a         2330 c         1765 g         3467 t
ORIGIN      
        1 aaattttgac cgaaattcat ttcatcactt tcctttcttt tccattgctc ggtccgccgc
       61 tgcacttgca tgcatcaagc acatcacagt aacaccaagc atactgccac caaacaccaa
      121 taaaatatct ttcacaattt ccatcacgat tcctcttttc atataatcaa attttcaatt
      181 gccatacttt gttgctccaa aatctttttg tgccggtctg atagtcgagt attatttacc
      241 atatcctgaa tgtacgttgt ctgattcaac cgatccaact ccttggccat cttccacgta
      301 ggggcgactt gtcgtccaag ccagttcaac gttcgttcaa tggtataagg cttcggttca
      361 gaagttaatt gaatttcctt ctgattccgt ccaatgaagc gcccccattg ttcattcaag
      421 ggccagttag ttcgcctttt gccttccacc ttatccgcaa atcgcaagta ccggttaata
      481 atggaaaatg tggtactttc aatactgcga ccttttaaca aatcttgaat tgcatgataa
      541 gctcgatcat ttttcagtcg gatttcaaaa cgatttttcg tttcggtctg gtctaaagga
      601 atccctaact tgatatattg ctcatagtct ttttcgtaca aacagaaata gacctcactc
      661 ttaagactgc caatatacaa agtattgccc ataccatctt tatcatcagg tttcaacagt
      721 tccccggaac gatagctttt gaaggtacga aacaaggaga tacattcttc cttctgacat
      781 tttttcgtta aatcaggaat atctaacagg ccaactcgat cattgattgc caggtctaag
      841 cgtttcatca cgccaccggc ttttagacaa tcttcgaaaa agtcatacca gctacgcttt
      901 tgggcgagta aaaaagtttc aaactgccga caaccacgac cctttaattc taaaaggacg
      961 cctttatctt cttcatgtga caacatcacc acaatatctc ccataacata ttgttcttga
     1021 taggagtaaa aggcgtaatc ttcgtggagc atataatcaa atttcaaatg gagaatctct
     1081 tgaatgattt tcctcgcatc cgttgtggga aagcgaatcc ttacatagtc aaacagtaac
     1141 gttaaaggta actcgggatt gaaactttcc agttggtcaa aaattttcct catggttttt
     1201 gtcgatgcgg ttacgccacc attttctatc tgatttaagt acgggcgact aagtccacaa
     1261 gtcgtagcta atttattttg tgaaacgcca tattcattac gccggtcttt taatagccga
     1321 taatctaaat tcctttgtgc caagttctca cctccaaatt taaggtgtta actttttcaa
     1381 atcagttaac atctacacaa attgcttatt atccttgata ggacagccat tccttatcgt
     1441 tgcttgctta ttttttgtgt ttttgtaccc ccctgttaga tacggggggt gagacatcat
     1501 ggccggctaa cgccgtccat aaacaggcac agcaccacgg aggatttctg cttcgcaaat
     1561 cccccgcggt cagtgcctat cagtggttta tttggccgtt actccgcggt aataccctca
     1621 atttccaact ctgcttttat ctcaaacaag tattggcgga tatcaggttc tttagcaaag
     1681 tccgcaattc gttccaattc actgatgact tgattcagct ccattcgagc atccgtttcc
     1741 tcatcaatta catacggatc atcagggact ttcaccccgt ttttgcgaat atcaaaattg
     1801 agccggtaca gttcttcttc caattccgga tctgactgaa tcttgccaat cacccagtag
     1861 attccccaat caactcatca taaatcatct gtttttacct cctttaatct tctagcaata
     1921 tcaaaaatga ccggaactgt tacagaattt ccagcctgct tatacaactg actatctgaa
     1981 ttgacttgag aagctcgatc aaaagcccag tccggaaagc cctgaagccg ccagcactcc
     2041 ctaggtgtta actttcgaat ttgatactga tccgtgacga ctgcttgttg gtctcctgtt
     2101 aataaggtat gcacactttg ttttcccaga cggcctcgac gacttgtgga attaggaaaa
     2161 gccaggttca cactgttccc tggcttcacc tcttgatatt ctgtttcgtt tttccgaagc
     2221 atgatcttcg ttccgccttg tttcgttgac aaagtaggcg ctgttccagt tatctcataa
     2281 acccgattgc cttgttcaaa atttcccagt aacatacccg ctacagaaat tccatcatct
     2341 accggcaatg cgattttgat tccttcatca ccggtcaagg taggcgctac gtcatctgac
     2401 ccatagactt gtccccccat tcctcgattg gaagggttga tatttaataa gtttctgatg
     2461 ttttgatcag atgttggaga cgatccaatg ataggaaata cttgttcggt acatgcgtct
     2521 cgagaatgtc cgataaggaa gatccgctca cggttttggg gaacgtaggc ctttgagtta
     2581 agcacttgcc attccacatc ataccccaat ccatccagcg ttcggaggat cgtctcgaac
     2641 gtagcccctc cttcgtgatt gagtaatccc cggacgttct caaggaataa aaacttaggt
     2701 tggagaatag cggcgaaccg agcgatttca aagaataaag tacctcgagt atcggaaaat
     2761 ccttgccgct ttcccgcaat tgaaaaagct tggcacggaa atccaccgca aaggatatcc
     2821 actggtccga gagattgaat aaactcgtct gatacacttg tgatgtcatg catttccacc
     2881 tccttacttg tatcatggat tgccttataa ctctggcgag caaaatcatc aatttcacaa
     2941 aagccgatac actggtgacc cgcttgttcc atacctaagc aaaagccacc aatcccagcg
     3001 aataaatcta aaaatgtcat aaatcacctc gtaaaaaaag cccaggattc ttgacactcc
     3061 taggctacat actattattt tatccttccg tattaacgtt taatccacca atgtcgctaa
     3121 tttcccttaa gaaatcatat ccttttggta caagtggcgt ataaaactca ctaatcacac
     3181 tccctcccgt atctacatag cctcgacctt tgatacgctt cataaagaat tttttatcaa
     3241 cctcgccaaa catcatggaa tatcccagtt cactcatacg acccaaagcc acccggaaat
     3301 taaattgatc gcgaatcccg tctcctaaat atttcgcatc cggtctttga caagctagaa
     3361 tcagaaagaa accagattga cgacctaaca tcacgatttg tttgagctta ttcaaaatca
     3421 ccgcactttc cttcgtcgtt aacatttcca tataaaccac gtattcatca aagattaaaa
     3481 agtttggtgg aagtccaaga aacgcataat tctctcctgt tttgtagttg ggcatttctt
     3541 tcattgcctt gctacgagtc atcatgcgct catagaaatc ttccacacaa gcagaaattt
     3601 cctccttttg ggaatagaca tgaggcatta ccgtacctaa atcggctaag tccgcatttt
     3661 tgggatcaag aacaaaaagc tcggcatcag attttaataa agcctcgatg atagtgagta
     3721 aaaagtaagt cttcccacca cctgtgccgc cagcgattaa catatgaggt aaagaatcat
     3781 aagcccatac ttgatttttc atcaatcgca aagccccact ttctgccact acttcgtcta
     3841 tcccaattcg attggcaatt atatcataca gtaaagtgta ctccacatag gagtctttga
     3901 gttccttttc tactagttca caatatagac cactttctaa cttcttttcc agctttaaaa
     3961 gctgatcttg atattttccc agtgaaattt gaacgcgaat agaaagtaaa ccatccttca
     4021 tacggtaata gattttgggg aagtaactga ttgtctcttt ggttcgacta ctattgaggt
     4081 ctttgaaaaa tccttcactc tgagtttgtt ttacttcgta ccaatgattt tctagaatca
     4141 tccgggctag cttttgtcga tgccacaaac tgcgccaatg atcctgaaaa aagtgtatat
     4201 aaagcaacat cgtgcttcct gaaagaacga gacttcctag caaatagatt aaatctttta
     4261 tagaacgaac gatttctata gtagaaagtg atagcttaga aatatcaaat tgtctaagct
     4321 cttgaaaata aaagacgctc atccaaccga tgaacagagc gagtagccag ccaagaacaa
     4381 agtgataaac caagtgctga tctcctgccc gtatacgatg ccctttgtac atttttatgg
     4441 tttccacctc caatcgaaaa aagcttcata ggatttcatt gatccaattt caagcagtta
     4501 tgctagtcat ttactgctat agggagttgt ttcgtttcca ctagttgctt taaaaaaact
     4561 tcaatggcat ccggcatttc cacaccaatt tcttccaata cggtttcggc ttctcgacat
     4621 agactttcat ttacttcgat aaccataaag aaggactctt cttcccctaa ccaattcagt
     4681 acatcaaaat cttcttcggc atcgttacct acttccagta acccttgatg cataatcaga
     4741 aacatttttt catcataggt taatgcttta gtgagttgtt cctccgttag ctcacctttt
     4801 ccatattgat cgagacaatc taaaatatgt tcaacattaa tttccatacg cttcacctat
     4861 ttctttcctt cattttggtt tgtagaattt ccctgatctg gttgtcgttt caagactaaa
     4921 tcatcggcct taatgaacca attgacagta gttccttctc ggaaaacata attggcgacc
     4981 gtatccatga ttggattgac caactctacc tctgcgtcat aggcaaactc gcgtaagggt
     5041 acatttgctg ggatactaac ttggatcatt cgaccttgcg cagaggattt caaattataa
     5101 gtccgttctt ttacctcgtt actaaccgtt ccatcctcat tttgcttccg tacttctcgg
     5161 cgtaatgcgg aaaatcgtaa tttcccaaac gttagtcctg aatctacaac aataccttct
     5221 gttaatctca tcttcttttc ctcctatgct ttcactaaat catcgacata caacacgtag
     5281 tcggtaaacc cacgattctc aatctcatag cctttggcaa ccaaatgggg attcacggct
     5341 tttagtggtt gatcttgatc aaaatccttt tcgccggctt ccgcagctac caccacttca
     5401 atatcatcgg ctcgttgctt actggaatac aaatgataac tacgaccaat tactgtagta
     5461 ttccgcccat atccttcagt taaaacttcg ccttcgccgg cataccttat ctttccaaag
     5521 gtttcggcca tatctggcac aacaaatttc aattccatct tcttttcctc ttttcatttt
     5581 tagttttgga tacaaaaata gcgcgaccac tttttttagg aggcggtcgc gcacgttggt
     5641 ttttcctatt tagttgtttc atcacatcac cttagtttcg gcgattccag aaataatacc
     5701 cagcagtcgc aaagatcaag gtaaacccaa taaacagcag aacattagaa tttttctccc
     5761 ctgtttgtgg gaatgtcttt actgtaggac tagattcttg agagtcactt ggaacagttg
     5821 gtatttccgg ttgttctggt gcacttggtg ttgtcggaac ttttttaggt gttaaagttt
     5881 gagttttttc ttttagatcc tcattgtgtt tcccattgat gtttccgtct ttgccatagt
     5941 taatttcttt gaaggtaaaa cttgtacctt ctggatattt acttgtgtcc actttttccg
     6001 caagtacggt tttagtgaat tctttgtcat tcacttcata atcaattttg ccagatttcc
     6061 aaatttcttt ggtcgtatta tccggaagta atgcatacag aattgtttca aaagcttctt
     6121 ttgagccatc cagtacatca tgggtaatcg acacatcatc gaacatatcc accacatccc
     6181 catgagtaaa ggtttgcgaa ccatcttcta ggtgggcttt tgtttggatg gaaacatggc
     6241 gttccaccgt acaattgaca gtttgggctt gattgttcaa gctagcatct ttcgctactg
     6301 gatcatcacc tgcttcatag ggtttcttgt tttcatacac atagttgaat aagacgatgg
     6361 ttttatccaa aacttgttct gcggttaatt tatgcaaaat tttccaagtt cctgttttct
     6421 ctttattcgc caacgttgcg gttgtttcgg caattaccac tggtttggct ttttcatctt
     6481 tttcttgggc agcttttgtc gcttccacat caatggcttg cgctaccaca taccagtctt
     6541 ctttgatttc atgcaagtta tagctcaatt tatcaactag ctcggtagaa tcaaggtctg
     6601 tcaattcttt gcctccgtct ttccaagtcc caagagaagt taagctatct tcttcttcag
     6661 gtaaatcata gagcatcaaa cgattcaagc taacagaaaa tgctttattc gtcagtttct
     6721 cgtaaggaac ggtcaccgtt ttaattggtt gtttttgtcc tttttcggta atcgtaaaaa
     6781 tgtattcact cttagtgtaa tccgctttat tttctttaaa agtagaacgg atttctaatg
     6841 gcgtaatttt ttgaaatcct tctggagcct ctacttcttc aagtaaataa tccccataag
     6901 gaagattttc aaacttgcca tagccatcaa aacctagttg ctcgttataa gctgtaattg
     6961 cttcatcttc ggcacctgta atctcactgg ttccttccaa tggcgatact ttaaaggtca
     7021 gatcattgaa gccagtttcg gcagtcccag cagctgatcc agcaaattta aagaaatcaa
     7081 agccaaagcg aatgacttgt tcttttgccg taacatctcg ggtaatcacg gcattttttt
     7141 cgttatcatc cacctttttg atggaaacag ggtactttgt ttcatccaag gtatatcctt
     7201 caggtgcttt ggtttcttgc cagtaatact cattaatcgc taagtgttta acagcaactt
     7261 gattcttttc atctaaagtc aaagtcaccg tttcatcaga agctttcgtt cccttcacta
     7321 actctggttt aaaagcctca ctccatttga ccgcttgacc atcttttgca gtaaagagag
     7381 tatattcagc tcctttaaac tcagctttac cttgagtttc attaccggta tctttgtctt
     7441 cttttgtcaa agtggtttca ccagtgattt cttggttttg cccttttacg ttactggtaa
     7501 caagagccac ggtttgattg acatagttca attcgacttt tactggttta aaggtattca
     7561 caaagccatt actagctttg gtttcggtca cataataggt tcccaattcc aacgcattgg
     7621 caatctcttt tggtgtttcc gcatgacctt tttcatccgt ggtcatttct tggacaattt
     7681 caccggttgg actgtctttg cgaatggcaa acgtatttcc ggctaatgag tatttgtcgt
     7741 tccaaagatc agttcctgtt tccaccccca ttttattcaa aatgatttga cctttttcgc
     7801 gagtattttt tgaagtcaca gaaatagtct caccagattt aatggttgcg gtgattggtg
     7861 tagtgtcaat cgtataagga gctggcactg atttttcagt aatggttact ttcgcgccat
     7921 gaggaatctc atccaatgtc gcaatgcctt ctttatcggt ggtcacgtct tttgcaggta
     7981 atgtttttcc aaagtctaaa tggaaaacgg ttcccggtac aacatttcct gtttctttat
     8041 cgactttttt aatcttcaaa gaaccctcag tttccacatc aattttgatt gtgtaggtat
     8101 tgggcttatc aattgccccc gccatcaaag tttgttgacc agccatttta taagctaccg
     8161 gtgttcctgt attagctgac ttctttaggg ttaattcacc actctctttt gaattggcat
     8221 ttggagtaat cactagttga ttgccgttca cacgataatc aatatttgcg atattctcga
     8281 caacttcatc aaactccgat aaattcaagc cgttcgtatc tgtcacagta gtcgattgac
     8341 ctaacacggt tttcactgtg gagttatgga agcttggttt cttttgataa tccgcaatgg
     8401 cttgattgat ttttgactca atggctgcga tatttactgc actaccatca gaacgtttaa
     8461 ttgagtgaag cgtagccatt aacttcttgc caaatcatct tctgggccac aatatgtgta
     8521 tccacatccg taccagcttc cttccataaa acggaaacta atttcgcttt ctccgacata
     8581 tcaggcaatg gatttttctc ataaccagga gtgaactcat tgtaaatcgg cacccctggc
     8641 tcaatacaga aaatatcttg cttcacacca ttgtaaactg catagtgcgg aacggtaact
     8701 tctgacatgg gacgaccatc gctaaatgta ccttcgacag catactggtg agccaaatcc
     8761 aattccacag ttaccgtttg tggatgagta atttcatcag caaaggcaat cgttgggcca
     8821 agcgatccac caattgtttg gcataataga ctcagtgttg cgagcaaacg ccaattctta
     8881 aatcttggct ttttcataaa ataaatctcc tttcgttttt gggtaataaa aaagacgtcc
     8941 aaattttaat tttgaacgtc tgatttagag tgatactatt tagttttctt tgtcttggat
     9001 aggttgtttc tctgcttata aattttgatt cttcgataat caaatccaat ctctgccatc
     9061 aattcctccc aatttttgtt cgttcttttc ataattgtat caactgatgg tagttttggg
     9121 gattgaacta gttttcgaaa ctttgtcatt gataaatcat cctgtagttt taatcggatt
     9181 atttcatttt ttagttctaa cagcagttcg aaatcagtaa atcgagatcc atcatttttt
     9241 tctttaaaaa gtggacgtat atctcctaac atctttttaa tggtactaag tgaaggaata
     9301 ttcttcccta ctgatttttg ttcgtacatt cgttgagagg ttatcttctc gcacttaata
     9361 aaggcttcta ctatttccaa taattcttgt tctgacattc tagcccattt tccataccca
     9421 gtattttctc gtcctatact aatcaataaa ttctcccatg atccatattt tttgttaatg
     9481 aaccacatac ttggtgcaga atttggattt tctgatagct ttttctggta ttgacggcta
     9541 gatgtaattc cgtgtttttc gatgaattca ttaattgtta aaatcagttt taactcactt
     9601 tgatttttcc aaatgatacg tccatcattt tgcttcacga taaaaactcc taatagttat
     9661 ttagatagaa gcctatcaca tcaacttttt agtagaaaag tacatgtttt gtcttttttg
     9721 ctaatagctg accctttgag aaacttgggt tcaaaagttg ctaaattcgg taaatgtttt
     9781 ggtatttttc gtattatatt tgtcgtcaag ctgactacta aattttgccc tattttgacg
     9841 acactgatag tcaatgtcgg acaatctagg acaattttta agggaatatg attgaaaaat
     9901 ggtccaaaac agccaaaaat ggcttaaaac tgtgatagag aaaaaagaaa aagcccttga
     9961 tatacaagga cttttcacaa atttcagatt tccaaggaag ttttccgtgt ttatcttctg
    10021 atttctttga tacgagcagc ttttccgtgt aatgcacgta aacaaaacca ctatattcta
    10081 ttatgtatct aataccctca aaacagctat aaatcaacaa taccgacata taataaacac
    10141 tatgttcaat ctgagatata tcaaagataa acgtattttt gccccctttt tgcccctcaa
    10201 atatcgcttt acaacgagaa cgtaagttcg tataatcgtt ttgaggtgat ttttatgatg
    10261 gaagacttta ttagaaaaaa tatcagtgat gaatatgcag acttttatga gcaaagcagc
    10321 aaaaaagata aattccagat ggatgtttca attttagcta tattagcttt ttccgaaaat
    10381 aaacaacctg taactgcaaa aaaagaaac
//
LOCUS       DACDKM010000097        10403 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20834_2016
            SAMN12640370-rid9891663.denovo.097, whole genome shotgun sequence.
ACCESSION   DACDKM010000097 DACDKM010000000
VERSION     DACDKM010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640370
            Sequence Read Archive: SRR10040891
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10403)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10403)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742835.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742835.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:04:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,012
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 29 of 75
            Pseudo Genes (incomplete)         :: 33 of 75
            Pseudo Genes (internal stop)      :: 19 of 75
            Pseudo Genes (multiple problems)  :: 5 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10403
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640370-rid9891663.denovo.097"
                     /isolate="E20834_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(<1..399)
                     /locus_tag="IV334_002237"
     CDS             complement(<1..399)
                     /locus_tag="IV334_002237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8944289.1"
                     /translation="MNDEYKNDEDKMLFEEIENRCRLNFELRGKMSLIQQKKYLANKS
                     EFTLGHVEKLISDWISSRSEFTKIKQPIKFDMKKLLLNKSEIGNRDQYIRAKGQEIID
                     SLGEMRSYNYLYVTHRADGMVITVGKSSSND"
     gene            complement(499..1008)
                     /locus_tag="IV334_002238"
     CDS             complement(499..1008)
                     /locus_tag="IV334_002238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1456 family protein"
                     /protein_id="HAP8944290.1"
                     /translation="MNNNDRLLRLRYAIDLKDTDLIKAFELGGVPLTKEEAQAVLTKV
                     QDKNKDNAENNVYEKTINNQVFDAFLNGLILLARGPQKDKPIVDTPQKSKEIKYINNV
                     LLKKLKIALSMTTDDILDVFAEAEIYPSKGEIGAFLRKEGQRNFKPCGDKYMRNFLKG
                     LGIYNRRKV"
     gene            complement(1209..2078)
                     /locus_tag="IV334_002239"
     CDS             complement(1209..2078)
                     /locus_tag="IV334_002239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HAP8944291.1"
                     /translation="MYGSIEAGGTKFVCAIGNDDLKVLERVSFPTTTPNETMSLVIDF
                     FNHYKEQLESIGVGSFGPIDIHRESKTYGHITSTPKTAWKNFDFVGTLNKHFEIPIAW
                     TTDVNAACYGEYVSGHGKGLSSVVYYTVGTGIGGGAIQDGIFVEGFSHPEMGHTLVKR
                     HSGDTFSGTCPFHHDCLEGLASGPAIEMRTGTKGQDLSIEDPFWEIEADYIAQCACNT
                     TLMLSPDIIIFGGGVMQQEHLKKKVQRRFLELINGYVDTPNIEEYIVTPKLADNAGTI
                     GCLTLAKDVRINS"
     gene            complement(2098..3219)
                     /locus_tag="IV334_002240"
     CDS             complement(2098..3219)
                     /locus_tag="IV334_002240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2961 domain-containing protein"
                     /protein_id="HAP8944292.1"
                     /translation="MNCLNNIWLHKGEESRTISPENKNGEKGKAAMTASHLGIGRKGS
                     PSVPINAGETITLAEMEGPGILRHFWMTVTDKTIAGSFVLRDLILRIYWDDESTPSVE
                     SPLGDFFCNGFGTRCEVNSLPIVVNPTSGMNCYFEMPFRKKAKITLTNEHPEFIKHIF
                     YTFNYALTEVPDDAMYFHAKWNREPITTKGKDYTLIDGIKGTGYYVGTYFALCALERY
                     WWGEGEFKFYIDGDEDFPTITSTGAEDYFGGAWAFHKKNFGERAYAQNFNTLFMGYPF
                     QSKRDHTRDYFSAGKPNPNPVHGFGDDALPMHGLYRWHLPDPISFKENLRVTFQTLGN
                     DDIKLYERQDDISSVAYWYQKEPHEFFEKLLDREERIPR"
     gene            complement(3221..3616)
                     /locus_tag="IV334_002241"
     CDS             complement(3221..3616)
                     /locus_tag="IV334_002241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HAP8944293.1"
                     /translation="MRRLIIASHSILAEGFKETLEFLTGMHNQISSLCAYVEEIDDID
                     EQIKELVDTTDEIIVLTDMAGGSVNQKFFTYISDRVHVIAGMNLPLAMSIALQLGNDN
                     LDIGAIIAEAQQQIIYMNNQKSEERIEDE"
     gene            complement(3635..4432)
                     /locus_tag="IV334_002242"
     CDS             complement(3635..4432)
                     /locus_tag="IV334_002242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010238514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HAP8944294.1"
                     /translation="MSEKYQVTKKDLRKANYRWLMSVCTFNYQTQQGASVTYALSPIL
                     RKLYKDDDEYIEALNNHFQYYNTQPWLAAIILGACVAMEEKQGLEAKDAINDFKVGTM
                     GPIAGIGDSLLMTMIPTIMGSIAAYMALENNPVGIFLWFILIMVIFFFRMRGFEFGYK
                     QGMKIVTEYGEKINYLTEAASVLGLTVVGSLIASVISVSTPLKFTFGEVSMKIQPMLD
                     KILPTLIPVIITAAAYYLLSKKKISMTILILLVIVFAMVMAAFGILA"
     gene            complement(4434..5222)
                     /locus_tag="IV334_002243"
     CDS             complement(4434..5222)
                     /locus_tag="IV334_002243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAP8944295.1"
                     /translation="MHFTIFQIIMLTILAFIKHVDYYGIPMIFVNYAVFWGLFTGILM
                     GDIKTGLIVGGTVQLMSLGVAGFGGSSVPDYGTCAIIATAFGSQLGVEAGLAIGLPVG
                     MLGVQLDVIVKILNGFVVRKSQEYANKREFKKMNSVLWLGPIFFGLSAAIPVFIAVTL
                     GQSAVNYVLDVLPNWFMDGLTLAGKMLPAVGIAMLLRFMPTGRYISYLLVGFFLAAFL
                     KVPIIGAAIIGFALAFNVYHRAEKDAQITASAGASDVGFIGEDE"
     gene            complement(5236..5712)
                     /locus_tag="IV334_002244"
     CDS             complement(5236..5712)
                     /locus_tag="IV334_002244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAP8944296.1"
                     /translation="MSIVMSRVDNRLLHGIIVTQWAPMSKANRVMVIDDSVANNDIQK
                     SSMRLARPAGMAVSIITEETAIANFKKGKYDQEKVFVIVKEPETILHLVDNGIRIDRL
                     IVGGTVVKDNALQLSSRAYASDENISTYKKLLDSGVEIVTQFVPADKAQEIQSKLG"
     gene            complement(5784..8441)
                     /locus_tag="IV334_002245"
     CDS             complement(5784..8441)
                     /locus_tag="IV334_002245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAP8944297.1"
                     /translation="MEELKERIVNYVIENSEIKNKWGNNTAQKISEKLNVSRNIVSQY
                     LNEFVKDGEFGKINTRPVIFFSLPEKVPNQQIFSSSEEAEKWYDSSFRVGIERVIGSQ
                     GSLRNVIAQIKAAIYYPPNGLPILLHGETGTGKSYLANYVYKDLLANNYIEEKASFIS
                     VNCSEYANNPELFLSNFFGHVKGAYTGAEDGRKGLAELANNGVLFLDEIHALSNECQE
                     KLFQFMDTRKFHRMGDNENWYTSSCWIIFATSEPPEDKLLSTLLRRIPVKLTLPTLDS
                     RSLYERKQLIKIFFKSEEERLAKKILISQQLLDFLLNYRFSENVGNLKNIIQLTCANA
                     FYFLDDLEELYIQMYHLPQWVNFNGMKVMKRQNELKYIQMDDLEKVDEVFNINELIEY
                     LYQTWKKDTDKQQLFEKYDRYFRQKNVDIQNNSALTYPTYQEIRFRQYVKEIERDFQI
                     EFDYYYLQITSIFLNSWIYQIQLTSDDKLYSFINDINSWCSREVQLVNRFIQIGDIYL
                     TKIQKNVLVILFKLMCSKEFQQKRLALILAHGDLTATCIASTANRIVERYIYDAIDMP
                     LMTSSSEIADKINRYLQECTGYEEIILLVDMGSLEKIYLNLELDAQKKFGLLNNVSTR
                     MAIDVANQILLDLPLKQILEQVKENQNVDYIYESSNIKKDMIVCSCASGLGTSFKLKK
                     ILEDSFPRDTDLVIETCDYYSLVNENYLSELRKENNILFIVGTLNPHLEDVEFFSIED
                     MILGMNISKFSNQLNRHFNDNELKVLNNNILKNFSLTNLMEQLTILNPTKLLERVSDA
                     IYILQNDLGISFDNNTCFGLYVHISCLIERLVKQNTLEDEIYFNETSEEFQKFQTHFK
                     QSFSVVEHYYSVDIPIHEVKYVYDYVKRA"
     gene            complement(8460..8864)
                     /locus_tag="IV334_002246"
     CDS             complement(8460..8864)
                     /locus_tag="IV334_002246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit M"
                     /protein_id="HAP8944298.1"
                     /translation="MGAELNQKLFSAADNLRSKMDASEYKNYLLGLIFYKYLSDRLLE
                     QVVLLADESLEEYDTVSKQTMLYRELLSDEESKEDLIATIVDILGYAISPEYLFNVLA
                     DQAKQATFQLNDLNKAFLASHIYGKILSAKFE"
     gene            complement(9096..9353)
                     /locus_tag="IV334_002247"
     CDS             complement(9096..9353)
                     /locus_tag="IV334_002247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase"
                     /protein_id="HAP8944299.1"
                     /translation="MEDSPELFPMLKDRLNELTEKRRQLQIRENEILGIFQQQGEPIQ
                     VKDVQQILTSLDCFLIQSEKKQVKALYHTFIEKITFDPNQK"
     gene            complement(9478..9810)
                     /locus_tag="IV334_002248"
     CDS             complement(9478..9810)
                     /locus_tag="IV334_002248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8944300.1"
                     /translation="MPLDLSTGRKIRFSEIQYEGIGFTLIDLKNSTFEIKEGLDLKNL
                     LCADDKIKKIQLYKVPILTPTVLTMLEMENNINYIDSGSDLLIRWQVITNFLMELKKY
                     SNTQTKFS"
BASE COUNT         3232 a         2111 c         1455 g         3605 t
ORIGIN      
        1 atcattagaa gaagatttcc ccacagtaat gaccattcca tcagctctat gtgttacata
       61 caaataattg tatgacctca tttcacctaa tgaatcaatt atttcttgcc ctttagctct
      121 aatatattga tctctattac ctatctcact cttatttaac aaaagttttt tcatgtcaaa
      181 tttaatcggc tgttttattt ttgtaaattc gcttctacta ctaatccaat ccgagattaa
      241 tttttcaaca tgtcccagag taaactctga tttattcgct aaatactttt tttgttgtat
      301 taaagacatc tttccccgaa gctcaaaatt gagcctacat cgattctcta tctcttcaaa
      361 aagcatctta tcttcatcat tcttatattc atcattcata aaaccaatcc agctcctcga
      421 ttaagataat attttaatct catacaaaaa ctagctctca tcaaaacaac cattgttttg
      481 atttctcatg aaatttcttt atacttttct ccgattataa attcctaacc ccttgagaaa
      541 attacgcata tatttatctc cgcaaggctt aaaatttcgt tggccttctt ttctcaaaaa
      601 tgccccgatt tcgccttttg atggatagat ttctgcctca gcaaaaacgt ctaaaatatc
      661 atcagtggtc atagacaagg cgatttttaa tttttttagc aacacattgt tgatatattt
      721 gatttccttg cttttttgcg gcgtgtctac tatcggctta tctttttgcg gaccacgcgc
      781 caagagaatc aggccattta ggaatgcgtc aaaaacttgg ttgttgatgg ttttttcata
      841 cacattattt tctgcattat ctttgttctt atcctgtact ttggtcagta ccgcctgagc
      901 ttcttcttta gtcagtggta caccgcctag ctcaaaagct ttaatcaaat ccgtatcttt
      961 caaatcaatc gcataacgca atcgcaataa cctatcattg ttattcattt gttcctccga
     1021 agtatttagt ctttactttc ttacgtactt tttgtcttgc taagattttt aggatcaatg
     1081 ataaaaaccg catcacttat gataaggcgc tgggtatcat gagtgaatga tattgctgaa
     1141 tacaaatagt ccgaccacat taagttgatc ggacttcata agcattttat tgactaatac
     1201 ttttttgctt aagaattgat tctaacatct tttgctaaag ttaagcatcc tatagttcct
     1261 gcattatctg ctaacttagg tgtgacaatg tattcttcga tatttggcgt atcgacatag
     1321 ccgttaatta gttctaaaaa acgtctttgt actttctttt tcagatgttc ttgttgcatg
     1381 acaccaccac caaaaattat aatatctggt gaaagcatca gggtcgtatt acaggcacat
     1441 tgtgcaatat aatccgcctc gatttcccag aacggatctt ctatagaaag atcttgtccc
     1501 ttggttcccg tacgcatctc gatagcgggt ccagatgcca acccttcgag acaatcatga
     1561 tgaaaaggac aagtaccaga gaaagtatct cctgaatgtc tcttaactaa cgtatgcccc
     1621 atttctggat ggctaaaccc ttcaacaaaa atcccatctt gtatagctcc tccaccaatt
     1681 ccagttccaa cagtgtaata aacaacactg gataaacctt ttccatgacc tgaaacatac
     1741 tccccgtaac aagcagcatt cacatcggtt gtccaggcaa ttggaatttc gaaatgctta
     1801 ttcaacgttc caacaaaatc aaaatttttc caggcagttt ttggagtaga ggtgatatgt
     1861 ccataggtct ttgattcacg atggatatca atcggtccaa atgaccctac tccaatgctt
     1921 tctaattgtt ctttatagtg attaaagaaa tcaattacca aactcattgt ttcattcggt
     1981 gtagttgtag gaaaactcac tctttctaaa acctttaaat catcattgcc aatggcacaa
     2041 acaaatttag tcccacctgc ttctattgag ccatacattt cttactccac cgtcctttta
     2101 tctaggaatt cgttcttccc tgtctaatag tttttcgaaa aactcatgtg gttccttttg
     2161 gtaccaatag gcaacagaag aaatatcgtc ttgacgctca taaagcttga tgtcatcatt
     2221 tcctagggtt tggaaagtaa cacgtaaatt ttctttgaaa ctaatggggt cgggtaaatg
     2281 ccagcggtac aacccatgca tcggtaaagc atcatcgcca aaaccatgaa ctggatttgg
     2341 attaggtttt ccagcactaa aataatcccg ggtgtgatct cttttagatt gaaaaggata
     2401 gcccataaat agcgtattaa aattttgagc atatgccctt tctccaaagt tctttttatg
     2461 gaaagcccaa gcgccgccaa aataatcttc tgctcctgta gatgttattg tagggaaatc
     2521 ttcatctcca tctatataaa acttaaattc tccttccccc caccagtatc tttccaatgc
     2581 gcacaaagca aagtaggttc ctacataata cccagtgcct ttgattccat caataagggt
     2641 gtaatctttt ccttttgtcg tgatgggttc acggttccat ttagcatgaa aatacatcgc
     2701 atcatccggt acttcagtca atgcataatt aaatgtataa aaaatgtgct taataaactc
     2761 aggatgttca ttcgttaaag taatcttagc tttttttcga aatggcatct caaaatagca
     2821 attcatccct gatgttggat tcactacgat tggtaaggaa ttcacttcgc atcttgtccc
     2881 aaacccatta caaaaaaaat ctcctaatgg actttctaca gaaggagtgg attcatcatc
     2941 ccaataaata cgcaaaatta aatcacgtaa gacgaaactc ccagcgattg ttttgtctgt
     3001 cactgtcatc caaaaatgac gtaaaattcc agggccttcc atttctgcta aagtaattgt
     3061 ctctccagca tttatcggaa cagaaggtga cccttttctc ccaattccta gatgactcgc
     3121 tgtcattgcc gctttccctt tttcaccatt tttattttct ggtgaaatag ttctactttc
     3181 ctctcccttg tggagccata tattatttaa acaattcatt ttattcgtcc tctatccttt
     3241 cttcactttt ttgattattc atatagatga tttgttgttg tgcctccgca ataattgctc
     3301 caatatctaa attatcattt cctaactgca aagcgattga cattgccaag ggaagattca
     3361 ttcctgcaat tacgtgcact ctgtcagaaa tataggtaaa aaacttttga tttacacttc
     3421 ctccagccat gtcagttaaa acaattattt cgtctgtagt atccactaac tctttaattt
     3481 gttcatctat atcatcaatt tcttcgacat atgcacataa gctactaatt tgattatgca
     3541 tacctgttaa aaactctagt gtctctttaa aaccctcagc tagtattgaa tggctagcaa
     3601 tgatcaatct tctcataatt caaatccttt tcctttacgc taatattcca aatgcagcca
     3661 taaccatcgc aaaaacaata acgagtagaa tcaaaatggt catactgatt tttttcttac
     3721 tgagtagata atatgctgca gcagtaataa ttactggaat taatgttggt aaaattttgt
     3781 ccaacattgg ttggattttc atactaactt caccaaaagt gaatttaagt ggcgttgaaa
     3841 cactgataac tgaagcaatt aatgatccaa ctactgttag ccccaatact gaagcagctt
     3901 cagttaaata attaatcttt tcaccgtatt cagtaacaat cttcattccc tgcttataac
     3961 caaattcaaa gcctctcatt cggaaaaaga agattaccat gattaaaatg aaccataaaa
     4021 agattcctac aggattattt tctaaagcca tataagccgc aattgatccc atgatggttg
     4081 ggatcatcgt catcaaaaga gagtccccaa taccagcaat tggacccatc gtaccaactt
     4141 taaagtcatt aatcgcatct tttgcttcta atccttgctt ttcctccatt gcaacacaag
     4201 cacctaaaat aatggctgcc agccaaggct gtgtattgta atactgaaag tgattattta
     4261 aagcttcgat gtattcatca tcatctttgt ataattttct taaaataggt gacaatgcgt
     4321 aagtcacaga tgcaccttgc tgtgtttgat aattaaaggt acaaactgac atcaaccaac
     4381 gatagttcgc tttcctaaga tcctttttcg ttacttgata tttttcgctc attttattca
     4441 tcctccccaa taaacccaac atcagaagct cccgcagaag cagtgatttg tgcatctttt
     4501 tcagctctat gatacacatt aaatgctaaa gcgaatccta tgatcgccgc tccaataata
     4561 ggtaccttca aaaaagcggc taagaaaaat ccaacaagta aataactaat gtatcgacct
     4621 gtaggcataa agcgtaacaa catcgcaata ccaacagcag gcaacatttt tccggctaat
     4681 gttagcccat ccataaacca attaggtaaa acatccaaaa cgtaattaac agcagactgt
     4741 cctaaagtaa cggcaataaa tactggaatt gctgcagata aaccaaagaa aattggcccc
     4801 aaccacaaaa cactattcat tttcttaaat tctcgtttat tcgcatattc ctgtgatttg
     4861 cgaacaacaa aaccatttaa gattttgaca attacatcta gttgcacccc taacatacct
     4921 acaggtagtc ctattgctaa tccggcttct actccaagct ggctaccaaa tgccgttgca
     4981 ataattgcgc acgttccata gtccggtaca gatgagccac caaaaccggc aactcctaat
     5041 gacatcaatt gaactgtacc gccaactatc aaacctgttt ttatatcacc catcaaaatt
     5101 cctgtgaata atccccaaaa aactgcgtaa tttacaaaga tcatcggaat cccataataa
     5161 tcaacatgtt taataaatgc taatatagtc agcataataa tttgaaaaat agtaaaatgc
     5221 attatcctta ctcctttatc ctaattttga ttgaatttcc tgtgccttat ccgccggaac
     5281 aaattgtgta acaatttcta caccagaatc caacaatttt ttatacgtgc tgatattttc
     5341 atctgatgca taggctctag aagatagctg taatgcatta tctttaacaa cagtaccgcc
     5401 aacaattagt cggtcaattc taataccgtt atctactaaa tgaaggattg tttccggctc
     5461 tttcacgatg acaaatactt tttcctgatc gtatttcccc tttttaaaat tcgcaatcgc
     5521 tgtttcttca gtgataattg atacagccat tccagcaggt ctagctaaac gcatacttga
     5581 tttttggatg tcgttatttg cgacagaatc atcgatcacc atcactctgt ttgctttaga
     5641 cattggtgcc cattgcgtga caataatccc atgcaataaa cggttatcaa ctctcgacat
     5701 aacaatactc ataaatactt cctcctttat ctttcatttt cttacaagca attaccatgc
     5761 caaaacaaaa agaacttgat aaatcaagct ctttttacat aatcataaac gtatttaacc
     5821 tcatgaatcg ggatgtcgac actataataa tgttcaacga cactgaaaga ttgcttaaaa
     5881 tgtgtctgaa atttttgaaa ttcctcactc gtttcattga aataaatttc atcttccaaa
     5941 gtattttgtt ttactaatcg ttcaattaag caactaatgt gaacatataa tccgaaacac
     6001 gtattattat caaatgaaat tcctagatca ttttgtaaga tgtatatagc atctgaaacc
     6061 cgttctaata atttagtcgg atttaatatg gtcaattgct ccattaaatt cgttagacta
     6121 aaattcttca gaatattatt gttcaaaact ttcagttcat tatcattaaa gtgtctattc
     6181 aattgattgg agaatttact tatattcatt cctaaaatca tatcttcaat tgaaaagaat
     6241 tctacatctt ctagatgtgg atttagtgtt ccaacaataa aaagaatatt gttctcttta
     6301 cgaagctccg ataaatagtt ttcattcact aaagagtaat agtcacacgt ttctatgaca
     6361 agatcagtat cccttggaaa gctatcttca agtatctttt ttaatttaaa cgaagttcct
     6421 aatccgcttg cacaagaaca gacaatcata tccttcttaa tatttgatga ttcataaata
     6481 taatccacat tttgattttc ttttacttgt tcaagaatct gtttaagcgg taaatctaat
     6541 aatatctgat ttgcaacatc aatagccatt cttgttgaaa cattgtttag tagtccgaat
     6601 tttttttgag cgtctagttc caaatttaga taaattttct ccagtgaacc catatcaaca
     6661 agtaaaataa tttcttcata tccggtacac tcttgtaagt atctattaat tttgtctgca
     6721 atctcactac ttgaagtcat tagtggcata tcaatggcgt cgtatatata tctctcaacg
     6781 atcctattag cagtacttgc tatacaggtt gccgttagat ctccatgagc caaaatcagt
     6841 gctaaacgtt tttgctgaaa ttctttacta cacattaact taaataaaat aaccaaaaca
     6901 tttttttgaa tttttgttaa ataaatatcc cctatttgaa taaaacgatt gactaattga
     6961 acttctcgac tacaccaact attaatatca ttgatgaagg aatataattt atcgtccgat
     7021 gttaattgga tttgataaat ccaagaattc aaaaagatag acgttatttg aagataataa
     7081 tagtcaaatt ctatttgaaa atctctctct atttccttaa catactgtct aaatcgaatc
     7141 tcttgatagg tgggatatgt taaagctgaa ttattttgga tatcaacatt cttttgtcta
     7201 aaatatctat catatttctc aaaaagctgc tgcttatcag tgtctttctt ccacgtttga
     7261 taaagatact ctataagttc gttgatatta aaaacttcgt ctactttttc caaatcatcc
     7321 atttgaatat attttaattc attctgtctt ttcataacct tcatgccgtt gaaattcacc
     7381 cattgtggta aatgatacat ctgaatataa agttcttcca aatcatctaa gaaataaaag
     7441 gcattggcac aggtcaattg aatgatattt ttcaaattgc caacattttc actaaagcga
     7501 tagtttaata aaaaatctaa taattgttga gatatcagta ttttctttgc tagtctttct
     7561 tcttctgatt taaaaaatat cttgattaac tgttttcttt cataaagaga acgactgtct
     7621 aatgttggaa gtgttagctt tacaggtatt cttctcaaca aagttgataa tagtttatcc
     7681 tcaggtggtt ctgatgttgc aaatataatc cagcaacttg atgtgtacca gttttcatta
     7741 tcacccatcc gatgaaattt cctcgtatcc ataaactgaa aaagtttctc ctgacattca
     7801 ttgctcaatg catgaatttc atctaaaaat aagactccat tattagctaa ttctgctaaa
     7861 ccctttcttc catcttcggc tcctgtatat gcacctttta catgcccaaa aaaattggac
     7921 aagaaaagtt ctggattatt ggcatattca ctacaattaa ctgaaatgaa agatgccttc
     7981 tcttctatat aattattggc caacaaatcc ttatacacat aatttgctaa atagcttttt
     8041 ccagttccag tctcgccatg aagtaaaatg ggtaagccat ttggcggata gtagattgct
     8101 gctttaattt gagcaattac atttcgcaga cttccttgac ttccgataac cctttctatt
     8161 ccaaccctaa aagagctatc ataccatttt tccgcttcct ctgaagagct aaaaatttgt
     8221 tgatttggaa ctttctccgg taaagaaaag aagattactg gacgagtatt gatttttcca
     8281 aattctccat cttttacgaa ttcattcaaa tattgagaga caatattacg actaacattt
     8341 aatttttcag atattttctg tgctgtattg ttcccccatt tatttttaat ctcagagttc
     8401 tctatgacat aattgacaat gcgttctttt aattcttcca tcacagcacc tcctcgacac
     8461 tattcgaatt ttgccgacag tatttttcca taaatatgac ttgctaaaaa ggctttgttt
     8521 agatcattca attggaacgt tgcttgtttt gcttgatccg ctaagacgtt gaaaagatac
     8581 tctggcgaaa tcgcataccc caaaatatca acgattgttg caatcagatc ttcttttgat
     8641 tcttcatctg aaagtaattc acgatacaac atcgtttgtt tgcttactgt gtcatattcc
     8701 tctaaggatt catctgctaa aagaaccact tgttccaaca aacggtcgga taaatattta
     8761 tagaaaatca gccccaataa ataattttta tattctgacg catccatttt actacggaga
     8821 ttatctgccg cactaaataa cttctgattt aattctgcac ccaaaaagat acactcctat
     8881 ctcttattta aagttgctat ataccctctt attttagcat actcagtttt agaaaaagca
     8941 aaaaaataaa gaaacttatt cttgataaca agctacgtga cgtatatccc taacacaaaa
     9001 atagccatat cctttggttt ggatacggct atttgatatt actcattcaa ttatcaaatc
     9061 acagtaggtg tgaatctcat cgggatcggc aatatctact tctgattagg atcaaaggtg
     9121 attttctcaa taaaggtgtg atacaaagct ttaacttgct tcttctcact ctggataaga
     9181 aagcaatcca aactagtcaa tatctgttgg acatctttga cttgaatcgg ttccccttgt
     9241 tgttgaaaga tccctaagat ttcattctct cgaatttgta actgtcttcg tttttcagtc
     9301 aattcattta aacgatcttt taacatcgga aacaattcgg gactatcttc caaagcagct
     9361 tcctgttttt gctctaatag ttagattttg cgtaccattt tcttctttta ttttccttcg
     9421 attgctgatt ttaatatagt ctaaaggaaa tttatattta ttgtattctt tataacttca
     9481 agagaacttc gtttgagtat tactgtattt ttttaattcc ataagaaaat tagttattac
     9541 ctgccaccta atcagcaaat ctgaacctga gtcaatataa ttaatattat tttccatctc
     9601 caacattgta agtactgttg gtgttaatat agggactttg tacaattgta tttttttgat
     9661 tttatcatct gcacaaagga gattttttaa atctaatcct tcttttattt caaaagttga
     9721 attttttaaa tctataagag taaaaccaat tccttcatac tgaatttctg aaaatcgaat
     9781 ttttctccca gttgatagat caaggggcag aaaacctttt attacattgt gagtgtcacc
     9841 atcatttaca atgtcattag gaattttgat tttagaattc tgtggagcac ctattggaag
     9901 ataataattc attataattt tgtagtcttc atgaggaaat aaagtacttt taggattgct
     9961 aagatattct cgtaccgaat ctaagaatgg cttatccatt gaatcttcat ccccatttaa
    10021 actaaaaaac attcctacaa ttgcttttgc aactctaagt gaattcattt tctttattga
    10081 atgtgttaaa tcagacttac cattctttaa acttgtattg ttataaagga aatttttatt
    10141 gtcttctgca aatctaatat attcctcaac atactcacta ccaaaaaaac tattacattt
    10201 actacataac gtattggaat ggcaacagtt tttttgacaa attttataag gtgcagaact
    10261 tctttccgta tgctattccg attgtccttg acaatgagcc tcctcggatg tgacgatctt
    10321 cggcaggagc taacagttaa agttagactg cctcgctagc gttagcacat ccgagctcat
    10381 tatcaaggat tcgctgcgcc aat
//
LOCUS       DACDKM010000098        10377 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20834_2016
            SAMN12640370-rid9891663.denovo.098, whole genome shotgun sequence.
ACCESSION   DACDKM010000098 DACDKM010000000
VERSION     DACDKM010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640370
            Sequence Read Archive: SRR10040891
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10377)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10377)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742835.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742835.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:04:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,012
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 29 of 75
            Pseudo Genes (incomplete)         :: 33 of 75
            Pseudo Genes (internal stop)      :: 19 of 75
            Pseudo Genes (multiple problems)  :: 5 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10377
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640370-rid9891663.denovo.098"
                     /isolate="E20834_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            245..2941
                     /locus_tag="IV334_002249"
     CDS             245..2941
                     /locus_tag="IV334_002249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAP8944301.1"
                     /translation="MEYKYRIDILQPIVYYAFRHIKLEEKMKKSAKDRVFDELIRLTG
                     NMSSVTAEKISKNLGISRQNASHYLTRLVEDKKVEKLPGKPVLWKPLDEYAVVDNTEQ
                     INEAFHSVVGHDGSLREVIQKCIAAVKYPPNGLSVLINGATGVGKSFLATKIFEYAIH
                     EQIIEKDAPFAILNCADYADNPELLSATLFGYKKGAFTGAEKDTDGLLATANNGYLFL
                     DEVHRLSKENQEKLFLFIDTGNYRPVGENVNWHSAKVRFIFATTEKGENYLLDTFDRR
                     IQISVMLPTFEDRPIRERLELIQLFFQNEADVLQKDIIVSREALSLMLSHPFSGNIGK
                     LKNIIKISCADVYSRTQEEPLTIGKNEILIQLNMLESEVESLPIASLLIKKNQKIKTD
                     RIKPFDYDSILEKITQLINEGNFNENFSSIKNEVQHLVHHIDKDSFLSMSRKLMIQMF
                     RKVWVSVLGKTYGLTTSGPVAEIISRSYVISPSYKTDLTGINRTLAANLPRTTYLCNQ
                     FLEHLPPLSEKDENFLRLLIVVTLSDYVDESIELKGLLLAHGDSTASSIQAVVNQLCG
                     NYVFEALDMPVESNVSEIVESTRNFVKRQVNTDSLVLIVDMGSLNQIYTQIKNDLKGE
                     LLLLNNLTTSIALDIGLKMTMNAPFKQIAEDAADKYSINVQYFEGFSQSSNIIISCMS
                     GLGISDKLKDVFRHHLSDESIEVFTKDYRELRELIDQNDETYFDKTKLVITTSDLPRS
                     FSIPNINVYDILDGDGAANLEHILSPEMKPAEFNRLIQELVKFFSLEGIADRLNFLNP
                     TIVMSEVETVISKYENYYHFTLNGRVKLNLYMHTALMMERLFLARTEKSEEPNYDLKP
                     DEDEFYYVSRSIFQSMEMKYNFKVNAYELSLLYELLGPYIQK"
     gene            3018..3506
                     /locus_tag="IV334_002250"
     CDS             3018..3506
                     /locus_tag="IV334_002250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAP8944302.1"
                     /translation="MFLKVGTGIAYIKVNRKKGINHMKKILIATHGHLASGFLSSIQL
                     LTGKVQEITVINAYVDECDFEQELNVFVNQVNEQDQIFVFTDLFGGSVNQKITKTFLE
                     KEIAVTILSGFNLPILLEIVLATKELTKEDIQELVNKCQQELKMTILDEVHVVNEEED
                     FF"
     gene            3518..4012
                     /locus_tag="IV334_002251"
     CDS             3518..4012
                     /locus_tag="IV334_002251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAP8944303.1"
                     /translation="MIKAVRIDERLIHGQVAMTWTKSLNLTGLVVASDEAATNDIQKM
                     TLKMAAPSNVKVIIKTVDDAIHLLKDPRAKDMRLMVLVPNVKDAVKVAKAFPTEIELM
                     NVGNAGKMTPPSEDKKTLSKEVMLTTSEIQDLKELVELYPDTVFQPTPAMEKRSAISI
                     LSDF"
     gene            4033..4842
                     /locus_tag="IV334_002252"
     CDS             4033..4842
                     /locus_tag="IV334_002252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAP8944304.1"
                     /translation="MYDAFVVALAVFIGIAGHEMFGMSMLSRPIVVAPLAGALMGDLQ
                     TGLIVGASLEAIFMGVVNIGISSTAEPSLAAGLATAFAIKMGGNVDAIIPIVFPLAVL
                     GLQLMNMIFSFVCGPMAAKFFKFAKSGDQKGIINLHFFMWFVHYALYALIPFFAVLFG
                     ADVVKSVLDNIPEVIMNGLTVAGNLLPAVGMAMLLRMLWSKNIAVWFFFGAVVMAYLE
                     LPLIAIAAVGTIIAIVIAQRDLQLKNISVHAVQSDAAVNATLDQEEEDFFQ"
     gene            4839..5660
                     /locus_tag="IV334_002253"
     CDS             4839..5660
                     /locus_tag="IV334_002253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010755513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HAP8944305.1"
                     /translation="MKLADGISKDEKKLVRKMFWRSMTMYASVNPVTMGGGGFAYSMM
                     PFINHFYKSEEDRAAALERHTQYFSTTIPCASFVMGIAASMEKENSEKAGFDVESINA
                     IKTSLMGPLAGIGDSLFWGVWRVVTAGLAINLANAGNILAPIIFLVMFNIPNYLMRYY
                     GGVLGYSLGSKYIEKLYANGLIEILTKAASILGLIMVGAMTISMVKFNTVLSFSMKGE
                     ELMNVQEILDSIFKGIVPLLVTLSCFKLLDKKVGVIKIIFGMVVLGILLSLLGIA"
     gene            5711..7810
                     /locus_tag="IV334_002254"
     CDS             5711..7810
                     /locus_tag="IV334_002254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase"
                     /protein_id="HAP8944306.1"
                     /translation="MTINEVIYSPKHPFFNAELNDGNVTTSNEYSYYRNIPDVKGLTF
                     DVSNQKTLANLDVCGTIQTLTFYRENLLTEEKPGVWVNKQLSQTNSLSLKVEVNGIVY
                     HLSESDHRIEVDVIQDCLPRYIHHYSTFKVIVVSFAPILANKRLSMLVQQVYIVNETH
                     ESLQVSVKDVPSYQEKYSDQQNVLISQKGNKDTIAQQEVASFAIAFVDPNAFEEVMTF
                     QESDIEKWLKETLNYFAQLFGQLSMPNDRVVHLYRRALYQSFSSFGMNRQGQVVGSNW
                     GSYPATNRIWNKDMYYASLPFTMFEPELCQKTVLWFDQYGVKFPGSKFTGGINHSLSN
                     SLSSALLASLYFEHTGDTKFFERCPQVLLNASRIIEDILAQRRSGEPMLFPSVWLSDA
                     FALGKYHTGSNLCLWKACIGLAEIFEALEQLSLSKRYKNIACKLKESICKQMTIDGPF
                     GEQFLEGIGDEEKTTYSVKHYQKPILEQGLIFLSDVIVDGKINLLMHDGEESDTTLMP
                     FYQFLDNKDHLYQNTMAFSASSFNPTYSEEIKGITWGLESGATFPGFITVLMSELHNP
                     QAFVTRLEELICLADLDGSWWWWPYRLEAQQGEVVRDFGCGKCGWASGLFVSLMISQY
                     FGLKFKKGVLQIDPVDNLTFSWKNLKFGQAFISIECTQSELSISNLSEKPLKISDTKK
                     ILHVEKGKTIHIQRRKR"
     gene            7807..8925
                     /locus_tag="IV334_002255"
     CDS             7807..8925
                     /locus_tag="IV334_002255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2961 domain-containing protein"
                     /protein_id="HAP8944307.1"
                     /translation="MSILKNITQFKKIQSRAITPENPTGEKGKAAMLPSELGPSRKGR
                     GAIPLTSGEETVIADITGTGIIRHMWMTIRDRTEFGSFVLRDLILRIYWDGSDSPAVE
                     SPLGDFFCNGFGERCEVNSLPIVVNPTGGFNSYFEMPFQTSAKITIENLHPKDMSSFF
                     YTINYALVDELEENSLYFHANWHRERITKLQEDYKLIDRLKGQGYYVGTYLALTCLER
                     YWWGEGEFKFYIDGDSEYPTVTSTGSEDYFGGAWAFHEYDEFGRPHAKNYSTAFLGYP
                     YQSNRDATRDRFETGKLNAVHAFGDDAMPRHGLYRWHLQDPICFTEDLSVTLQQIGND
                     DVRLFERQDDVASVAYWYSTEASGNTKRHFTDRERLPR"
     gene            8964..9842
                     /locus_tag="IV334_002256"
     CDS             8964..9842
                     /locus_tag="IV334_002256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HAP8944308.1"
                     /translation="MIYGAIEAGGTKFVCAVGTEELTILERVSFPTTTPVETMKQVID
                     FFKPFKEQLAGIGVGCFGPIDIQCKSTSFGHITSTPKLAWQNFNFVGTLKQYFNIPIE
                     WTTDVNAACYGEYVAGSGKGLAGVAYFTVGTGIGGGALFNGTFVEGFSHPEMGHMLIK
                     RHPKDNFSGNCPFHQDCLEGMAAGPAIEKRLGVKGQNLLADDSFWQIEAFYLAQCAYN
                     TTLMFSPDRIIFGGGVMKQEHMKKKVQDKFVELINGYVEIPPIDSYIITPELGDNAGI
                     IGGLALARKAVRNKQP"
     gene            10033..10185
                     /locus_tag="IV334_002257"
     CDS             10033..10185
                     /locus_tag="IV334_002257"
                     /inference="COORDINATES: protein motif:HMM:NF016526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8944309.1"
                     /translation="MRIFTGGVHLNSNILCYFMSVVIVIVAYQCMFIFRNVDFIILGL
                     MFEFFW"
BASE COUNT         3209 a         1697 c         2307 g         3164 t
ORIGIN      
        1 ggcccgagac gggctagtaa atctaagatt gaaaaagtta cagaaggata aagtaattgg
       61 aaactatgca aatatactta acccttctgt aatctattaa taaaactaat atatgaatcc
      121 agttcaatat ttgtagctat gaaaaaacat aaaaatcata ttagataaaa actaaattgg
      181 tagataaatt attttgtcag atatctgagg caaatattcg actatttgtg tttcaaagta
      241 aaaattggaa tacaaatata gaatagacat attgcagccg atagtttatt atgcttttag
      301 acacattaaa ctggaggaaa aaatgaaaaa gagcgctaaa gatcgagttt ttgatgagct
      361 gatacgacta acagggaata tgtcttctgt cactgccgag aaaatttcga agaatctagg
      421 tatctctcgt cagaatgcca gccattattt gacgagatta gtggaagata aaaaggtcga
      481 gaaattaccg ggaaagcctg ttttgtggaa gcctttagat gaatatgcgg ttgtagataa
      541 tacggagcaa ataaacgaag cctttcattc tgtagttggt catgacggaa gtctgagaga
      601 ggtcattcaa aaatgtatag ccgctgtcaa atatccgcct aacggactca gtgtattaat
      661 aaatggagcc acgggtgttg gaaaaagttt tttagccaca aaaatttttg aatacgctat
      721 ccatgagcaa attattgaaa aagatgcacc atttgctatc ttaaactgtg ccgattatgc
      781 ggacaatccc gaactgcttt cggcaacact gtttggatac aagaaggggg cctttacggg
      841 ggctgaaaag gataccgatg gtttattggc aacggccaac aacggctatc tttttctcga
      901 tgaggtccat cgcctatcga aagaaaatca agaaaagctc tttctattta ttgatactgg
      961 aaactatcgt ccagtcggtg aaaatgtaaa ttggcattct gctaaggttc gttttatttt
     1021 cgccacgacg gaaaaaggtg aaaattattt attagacacc tttgatcggc gtattcaaat
     1081 tagtgttatg ttgccaactt ttgaagatcg ccccattcga gaacgattag agctgattca
     1141 attatttttt caaaatgaag cggatgtttt acaaaaagat attattgttt caagagaagc
     1201 cttgtcttta atgttatctc atccctttag tgggaatatt ggtaagctga agaatatcat
     1261 taaaatcagt tgtgcggatg tgtatagtcg aacacaagaa gaaccattaa cgattgggaa
     1321 aaatgagatc ttgattcagt taaatatgct ggaatccgaa gtagaaagct tgccgattgc
     1381 tagtttattg ataaagaaaa atcaaaagat aaaaacggat cgaatcaaac cgtttgatta
     1441 tgattcgatt ttggaaaaaa ttactcagct gataaatgaa ggtaatttca atgaaaattt
     1501 ttcttcgata aagaatgaag tacagcattt ggtgcatcac attgataagg attctttttt
     1561 atcaatgagt agaaaattga tgattcagat gtttagaaag gtctgggtat ccgttttagg
     1621 aaaaacgtac ggattgacga cgagtggacc tgttgcagaa attattagca gatcctatgt
     1681 catttcccca agctataaga cagacttaac cgggataaat cgtacattag ctgcaaatct
     1741 acctcggacg acttacttat gtaatcagtt cttggagcat ttaccaccgc tctcagaaaa
     1801 ggacgagaac tttctaagac tgcttattgt cgtgacttta tctgattatg tagatgaatc
     1861 catcgagctg aagggcctgt tgcttgctca cggagatagt acggctagca gcattcaagc
     1921 agtagtgaat caattatgcg gcaattatgt ttttgaagcg ttggatatgc cagtagagag
     1981 taatgtgtca gagattgtcg aatcgacaag gaatttcgtg aaacggcaag tgaatacaga
     2041 tagtctagtg ctgatcgtag atatgggctc tttaaatcaa atttacacac agatcaagaa
     2101 cgatttaaaa ggggagttac ttcttctgaa taatttgacg acttccattg cacttgatat
     2161 tggcttgaag atgacgatga atgcgccatt taaacagatt gcggaggatg cagcagacaa
     2221 gtattcaatc aatgttcaat actttgaagg cttttctcaa agtagcaaca ttatcatttc
     2281 ctgtatgtcg ggtttgggga tttcggacaa attgaaagat gtttttcgtc atcatttatc
     2341 ggatgaatcc atcgaagttt tcacgaagga ctacagagaa ctacgtgaat taatcgacca
     2401 aaatgatgaa acttattttg acaagacaaa attggtcatc acgacttcgg atcttccacg
     2461 ttccttttca attcctaata ttaatgttta tgacatctta gacggtgatg gagcagccaa
     2521 tttggagcat attttgagtc ctgaaatgaa gccggcagaa tttaaccgct tgattcagga
     2581 gctagtaaaa ttcttttcgt tggaaggaat tgcggaccgt ttgaactttc tgaacccaac
     2641 gatcgtgatg agtgaagtgg aaacagtcat ttctaaatat gaaaattact atcatttcac
     2701 tttaaatggc cgtgtaaaac taaatctcta tatgcatacg gcattgatga tggaacgttt
     2761 attcttggct cggacagaaa aatctgaaga accgaattat gatttgaaac cagatgaaga
     2821 tgaattttat tatgtttcta gaagtatttt tcaatcaatg gagatgaaat ataattttaa
     2881 agtcaatgct tatgagcttt cgctgctcta tgaattatta ggaccgtata ttcaaaaata
     2941 aagtgtctaa aaaagtgtct aatctgtttt ttttaggtta gacacttttt ttaatgtgaa
     3001 ttaacgtgat taaaccaatg tttttaaaag ttggcacagg aattgcttat ataaaagtga
     3061 acagaaagaa aggaattaac catatgaaga aaattctaat agcgactcac ggtcacttag
     3121 cttcaggctt tcttagcagt attcaattac tgacggggaa ggttcaagag attacggtga
     3181 ttaatgctta tgtggatgag tgtgattttg aacaggagtt gaatgtattt gtaaaccagg
     3241 tgaacgagca ggatcagatt tttgttttta ccgatttatt tggcggaagt gtcaatcaga
     3301 agattaccaa aacctttttg gagaaagaga ttgctgtaac tattttgtct ggctttaatc
     3361 tgccaattct gctcgaaata gtattggcaa ccaaggaatt aacaaaggag gatattcagg
     3421 aactggtaaa taaatgccaa caggaattaa agatgaccat tcttgatgag gtacatgtag
     3481 taaatgaaga ggaagatttt ttttaggagg aagaaaaatg attaaagcag tgcgaattga
     3541 tgaacgatta attcatggac aagtagcgat gacttggaca aagagtctaa atttaaccgg
     3601 gttagtagta gcaagcgatg aggcggctac caacgatatt caaaagatga cattgaagat
     3661 ggctgcacca tcgaatgtca aagtcattat taaaacagtt gatgacgcga ttcatttgtt
     3721 aaaagatccg cgggcgaagg acatgcgctt gatggtttta gtccctaatg tcaaagatgc
     3781 ggtaaaagtt gcgaaagctt tcccaacgga aatcgaatta atgaacgtgg ggaatgctgg
     3841 gaaaatgaca ccgccatcgg aagataagaa aaccttatca aaggaagtga tgttaacgac
     3901 gtcagaaatt caagacttaa aagaattagt tgaactttat ccagataccg tttttcagcc
     3961 tacaccggcg atggaaaaac gttcagctat ctctattttg agtgattttt agaaaaagga
     4021 aggagaaaaa ttatgtatga tgcatttgta gtcgctttgg ccgtttttat tggaattgct
     4081 ggacatgaaa tgttcgggat gtccatgctg agtcgtccga ttgttgttgc tccgttagca
     4141 ggagcgttga tgggggattt gcagacgggg ttgattgtcg gggcgtcact agaagccatc
     4201 tttatgggtg tcgtcaatat cggaatctct agtacggcgg agccttcttt ggctgctggg
     4261 ctggctacag cgttcgcaat caaaatggga ggaaacgtgg atgcgattat tcccattgtc
     4321 tttccactag cagtattagg attacagtta atgaatatga ttttttcttt tgtatgtgga
     4381 ccgatggctg ctaaattttt caaatttgca aaatctggtg atcaaaaagg aatcatcaat
     4441 ctgcacttct ttatgtggtt tgtccactat gcgctttatg ctcttattcc attctttgcc
     4501 gttttatttg gagcagatgt tgtcaaaagc gtcttggata atattccaga ggtcatcatg
     4561 aacggattaa cagtcgctgg aaatctgctg ccggcagttg ggatggcgat gctgttacgg
     4621 atgctttgga gtaaaaatat tgctgtgtgg tttttctttg gggccgttgt tatggcgtat
     4681 ctcgagttac cattgattgc aattgccgca gttggaacga ttattgcaat tgttatagcc
     4741 caaagagatc tacaactaaa aaatatatct gttcatgcag ttcaaagcga tgctgctgta
     4801 aatgcaacgc ttgaccaaga agaggaggat ttctttcaat gaaattagct gatggaattt
     4861 ctaaagatga gaaaaaattg gttcggaaga tgttttggcg ctcgatgact atgtatgctt
     4921 cggtgaatcc ggtgacgatg ggcggcggtg gtttcgccta ttctatgatg cccttcatta
     4981 atcatttcta caaaagtgaa gaagatcgag ctgcggcgct ggaacgtcat acacagtatt
     5041 tttcgacgac cattccttgc gcgtcctttg tcatggggat tgcggcatcg atggagaagg
     5101 aaaattctga aaaagctggt tttgacgtgg aaagtatcaa cgcgatcaaa acgagtttga
     5161 tgggaccatt ggctggaatt ggcgattcat tgttctgggg agtttggcgt gtcgttaccg
     5221 cgggactagc gatcaactta gccaatgccg gaaatatttt ggcaccaatt atctttttag
     5281 ttatgttcaa tatacctaat tacttgatgc gatactatgg cggcgtttta ggctattcat
     5341 taggctctaa atatattgaa aagctgtatg cgaacgggtt aattgaaatt ctgacaaaag
     5401 ctgctagtat cttgggtctg atcatggtag gtgcaatgac gatcagtatg gtcaaattca
     5461 ataccgtttt gagcttttca atgaagggcg aagaactaat gaatgtccaa gaaatcctcg
     5521 atagtatttt taaaggaatt gtaccgttat tggtaacatt gagctgtttc aaattactag
     5581 ataaaaaagt cggcgtcatc aaaattattt ttggaatggt cgtcttggga attttattgt
     5641 cattacttgg aatcgcatag taggaagctg cagctactag gctgcagttt ttttagcaag
     5701 gagggaaaag atgactatta atgaagtaat ttattcaccc aaacacccat tttttaatgc
     5761 tgagttaaac gacggaaatg tgactacttc aaacgaatac agctactatc ggaatattcc
     5821 tgatgtaaag ggattgacct tcgatgtaag taatcaaaag acattagcaa accttgatgt
     5881 ttgcgggacg attcaaacgt tgacctttta tcgagaaaat ttactaactg aggaaaaacc
     5941 aggtgtttgg gtcaataaac agctctctca aaccaattcg ttatccttaa aggttgaagt
     6001 aaacgggatt gtctaccatt tgtcagaatc ggatcatcgt attgaagtcg atgtgattca
     6061 agactgtttg cctagatata ttcatcatta ttcgacgttt aaggtcattg tagtatcctt
     6121 tgctcccata ctggctaata agcgtttaag tatgcttgtt caacaggttt acattgtgaa
     6181 tgaaacacac gaaagccttc aagtttcagt gaaagatgtg ccttcgtatc aagagaagta
     6241 ttcggatcag caaaatgtgt tgatttctca aaaaggaaat aaggacacga tcgcacaaca
     6301 ggaagtggca agcttcgcca ttgcttttgt ggacccaaat gcttttgaag aagtaatgac
     6361 ttttcaggaa tctgatatcg aaaaatggct gaaggaaaca ttgaactatt ttgctcagct
     6421 atttgggcag ctgtcaatgc caaacgatag agttgtccat ctctatcgtc gtgcacttta
     6481 tcaaagcttt tctagtttcg gtatgaatcg acagggacag gtcgtcggca gcaattgggg
     6541 cagctatcct gctactaatc gaatttggaa taaggatatg tattacgcat cattgccatt
     6601 caccatgttt gaaccggagt tatgtcaaaa aacggttcta tggtttgatc aatatggcgt
     6661 taagtttcct ggttcaaaat ttacaggagg tatcaatcat tctctgagta attcgttatc
     6721 cagtgcgtta ctggctagtc tttattttga acatactggg gatacaaaat tctttgagcg
     6781 gtgtccgcag gttctactga atgcttcaag aattattgag gacatattag ctcaacgtcg
     6841 gtcaggggaa ccaatgcttt ttccatctgt ttggttatca gacgcctttg cacttggaaa
     6901 gtatcatacc ggcagcaatc tttgtctttg gaaggcctgt attggacttg cagaaatttt
     6961 tgaagcccta gaacaactga gtcttagcaa gcgatacaaa aacattgcct gtaaactaaa
     7021 ggaatcaatt tgtaaacaaa tgactatcga tggtcctttt ggtgagcaat ttttggaggg
     7081 aatcggtgat gaggagaaaa caacctattc agtgaagcac tatcaaaaac cgattcttga
     7141 gcaagggtta atctttttaa gtgatgtgat tgtggatgga aaaattaatt tattgatgca
     7201 tgatggagaa gaatcagaca ctacgttgat gcctttttat caatttttag ataataaaga
     7261 tcatttatat cagaacacca tggccttttc tgctagttct ttcaatccaa cctatagcga
     7321 ggaaatcaaa ggcatcactt ggggattaga atccggcgcc acttttcctg gctttatcac
     7381 tgttttaatg agcgagctgc acaaccctca agctttcgta acccgattag aagaactaat
     7441 ttgtttggca gatttagacg gttcgtggtg gtggtggccg tatcgcttag aggctcaaca
     7501 aggagaagtc gtgcgagact ttggctgcgg caaatgcggc tgggcatcgg gtctttttgt
     7561 cagcctaatg atcagtcaat atttcggatt gaaattcaaa aaaggagtat tgcaaattga
     7621 tcctgttgat aatttaacgt tttcttggaa gaatttgaag ttcggtcaag ccttcatttc
     7681 gattgaatgt acgcaatccg agttgagtat ttcaaatttg tcagaaaagc cattgaaaat
     7741 aagtgatacg aaaaaaattc ttcacgtaga aaagggaaaa acgattcaca ttcaaaggag
     7801 aaaaagatga gtatactaaa aaatattacc caatttaaaa aaatccagag tcgagcaatc
     7861 actcccgaaa atccaacagg cgagaaaggt aaagctgcga tgttgccaag cgagctagga
     7921 ccaagccgca aaggtcgtgg cgcaattccg ctgacttctg gtgaagaaac tgtgattgcg
     7981 gatattactg gaacgggaat aattcgtcat atgtggatga cgattcgtga tcgaacagaa
     8041 tttggcagtt ttgttttacg tgatttaatc ttaagaattt attgggatgg gtcggatagc
     8101 cctgctgttg aaagtccttt aggggatttc ttttgtaatg ggtttggaga gcgttgcgag
     8161 gtgaactcgt tgccgatagt ggttaatcct acagggggct ttaattccta tttcgaaatg
     8221 cctttccaaa catccgctaa aattacgatc gagaatttac atccgaagga tatgtcctca
     8281 tttttttata cgataaatta cgctttggtg gacgaattgg aagaaaatag cctatatttt
     8341 cacgcaaatt ggcatcggga acgcattacc aaattacagg aggattataa gttaattgat
     8401 cgattaaagg gtcaaggcta ttacgtgggt acttaccttg cgcttacctg cctagaacgt
     8461 tattggtggg gcgaaggcga gtttaaattc tacattgatg gggatagtga gtatccgacc
     8521 gtaacctcaa caggttcaga agattatttt ggcggggcgt gggcctttca tgagtacgat
     8581 gagttcggac gtccacatgc gaaaaactat tcaacagcct ttttagggta tccttaccaa
     8641 tcaaatagag atgccacaag agatcgtttt gaaacgggaa aattgaatgc cgttcatgcc
     8701 tttggtgacg acgcaatgcc acgacatggt ctttatcgtt ggcatcttca agaccccatc
     8761 tgttttaccg aagatttatc ggtcacttta caacaaatcg gcaacgatga tgttcgctta
     8821 tttgagcggc aagacgatgt tgctagtgtc gcttactggt attcaacaga agcttcagga
     8881 aatactaaac gtcatttcac tgatcgagaa cgcttgccgc gttaatgaag tgtttttcac
     8941 atattcgaag ggaggaacct atgatgatat atggggcaat tgaagctgga gggactaaat
     9001 ttgtctgtgc agtcggtact gaggaattaa cgattttgga aagagtcagt tttcccacaa
     9061 caactccagt tgaaacaatg aagcaggtca ttgatttttt taaacctttt aaggaacaat
     9121 tggcaggcat cggggtcggc tgctttggtc caattgatat tcaatgcaaa tcaacgagtt
     9181 tcggtcatat cacatcaaca ccaaaacttg cttggcaaaa tttcaatttt gtaggaacgc
     9241 taaagcaata tttcaatatt ccgattgaat ggacaacaga tgtcaatgcg gcttgctatg
     9301 gtgaatatgt tgctggttct ggcaaagggt tagcaggtgt tgcatacttc actgtgggaa
     9361 caggtatcgg aggtggtgcc ttgttcaatg ggactttcgt ggaagggttc agtcatccgg
     9421 aaatggggca tatgttgatc aagcgacatc caaaggacaa tttttcagga aactgtccct
     9481 tccaccaaga ttgtttagag gggatggcag caggaccagc gattgaaaaa cggttgggtg
     9541 taaaaggcca gaatttattg gctgacgatt cgttttggca gattgaagcc ttttaccttg
     9601 cccaatgtgc atacaataca acactgatgt tttcgccaga tagaattatt tttggcggtg
     9661 gtgtaatgaa acaagaacac atgaagaaaa aagttcagga taaatttgtt gagcttataa
     9721 atggatatgt tgaaatacca ccaattgata gctacattat tacgcccgaa ttaggtgata
     9781 atgccggaat aatcggcgga ttagcattgg ccagaaaagc ggtaagaaat aagcaaccat
     9841 aattaatgaa tgtttcattg atactcatgt caaaagtatt taaccgagta atcaaatgat
     9901 caaatgaagt tggataaagc gaaaatgaat tcaaataaaa aggctctaag tcaaatcgtg
     9961 ttgttttcat taaatcactg aaaggaaatg cttctgcgtt tccttttttc tttttgttgt
    10021 gcgctgtata ccttgaggat attcactggt ggcgtacact taaatagtaa tatattgtgt
    10081 tactttatgt ctgtggtaat agttatagtt gcttaccaat gtatgttcat ttttcgaaat
    10141 gttgacttca taatccttgg cttaatgttt gaattttttt ggtgatagat tttttcagcc
    10201 gtataaaata aatcgtaatt ctattataga gagttgtgat tataaaaaga tagcaagaaa
    10261 aaaaaagaaa gtttctatac attttaaata ttttattcct atttctattg ctaaaaaagt
    10321 agaaattcta tatttacctt taactctcgc ttctcttgca aatgtagtta gcatttt
//
LOCUS       DACDKM010000099        10193 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20834_2016
            SAMN12640370-rid9891663.denovo.099, whole genome shotgun sequence.
ACCESSION   DACDKM010000099 DACDKM010000000
VERSION     DACDKM010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640370
            Sequence Read Archive: SRR10040891
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10193)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10193)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742835.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742835.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:04:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,012
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 29 of 75
            Pseudo Genes (incomplete)         :: 33 of 75
            Pseudo Genes (internal stop)      :: 19 of 75
            Pseudo Genes (multiple problems)  :: 5 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10193
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640370-rid9891663.denovo.099"
                     /isolate="E20834_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            121..627
                     /locus_tag="IV334_002258"
     CDS             121..627
                     /locus_tag="IV334_002258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HAP8944310.1"
                     /translation="MIFTGYKKKRQIEKYRHLLNGLIETDYQKMFRITMNYVHDKEEA
                     LDVMQDSFHKALNYFEKEKEIEHFSAWFYRILIRTALDSWRRQKRTPVDLFEIQDIPQ
                     QLPLETSVAELHMILDKIDSPEKEILILYFFEGFRLKEIATILDMNENTVKTKMYRSL
                     NALRQILE"
     gene            644..1543
                     /locus_tag="IV334_002259"
     CDS             644..1543
                     /locus_tag="IV334_002259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3298 and DUF4163 domain-containing protein"
                     /protein_id="HAP8944311.1"
                     /translation="MYSQKENLKKLKKTYKEQAIPKGLKEEIRQRFIIEEQSFIKAKR
                     RKRRLQGFGVSLAIVMITTSSLLFNSQVRSFAEDLPILGSVIELILGERFTDRSEKID
                     IQVPKINTQNEKENKTIHGLNQKYFREGQADFEKAEKEYGNFETDHYQVLGDYQKIVD
                     DNRFLVIERQITQTAADSHVEKRYDTIDKKNSVQLSLPLLFKDDTYLSVLTKEVKRQM
                     AEQVKEDPSKYYWTEQDIQEGTIEKPTLVTPTRSFYLNKEHQLVLTFSQYEIAPGYMG
                     TPEFVIPKSVTKTILASEDYLDH"
     gene            1630..2514
                     /locus_tag="IV334_002260"
     CDS             1630..2514
                     /locus_tag="IV334_002260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HAP8944312.1"
                     /translation="MKRNYLVISIIALSVLLFFEITSVASLITEVKGQTHSSEHSSQS
                     FKQSEQSTIKSQSTETANVKEAIQESAEKVQSQSSQSTQDSTKTSRDPAVKSVAISFD
                     DGPGATTTPQLLRILKEKNVHATFFVLGENTAQHPEIVKQTAEAGHEIGNHTYDHQNL
                     ATLSAQSMTEEVTKADTEIKKVTGKTPTFVRPPYGSVTNVGATIIQRPIIEWSVDSED
                     WKTRNPDLILQKIQATVYDGAIILFHDIYPETIRAVPQVIDYLKEQGYRITTVGDLLG
                     HPTAVENYYGRNDHRPVQ"
     gene            complement(2706..2777)
                     /locus_tag="IV334_002261"
     tRNA            complement(2706..2777)
                     /locus_tag="IV334_002261"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2742..2744),aa:Arg,seq:ccg)
     gene            2837..4171
                     /gene="rpoN"
                     /locus_tag="IV334_002262"
     CDS             2837..4171
                     /gene="rpoN"
                     /locus_tag="IV334_002262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HAP8944313.1"
                     /translation="MKFEQNFSQKQQQTQKLAMTQQLQQSIQVLQYNSEELLAFVENQ
                     AMENPLVEVVEPEWQPDYIKASSSSYEGEETNYLNQIPDTKGSLFDSLIEQVHLNYRD
                     TFLRKIVLYLVEYIDLNGFLTISLEEAGKEIGATPIQMLDALTLIQQLEPAGVGARSL
                     QECLMLQTERDDYAPELAYIVLEECFEELVERKWKEIAQRFSVDLHAVQQIFDYLQTL
                     SPSPGRIFDRSSELYIIPDVRVLVDDDKNVQVISNRKNQPNIRFQESYFKQMSQQADK
                     ETENYLKERKQEFEWLKKTILQRGDTILRVAQVIVSRQKAFFIDKERPIKPLTLKEVA
                     TEIDVHESTVSRAVNGKYLETDFGVFELKKFFTTRIPTNSTEQTEDLSADTAKKKLQE
                     LVDQEDKNKPLSDQKLVELLKKDEIAISRRTVAKYRDLLGIPSSSKRKRYDN"
     gene            4374..5411
                     /locus_tag="IV334_002263"
     CDS             4374..5411
                     /locus_tag="IV334_002263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SorC family transcriptional regulator"
                     /protein_id="HAP8944314.1"
                     /translation="MLDEIKMIESVAPDIIEVLQERYKILRNIYWMQPIGRRSLSESM
                     GITERVLRTETDLLKNLNLIDTSKSGMTLTTKGEEVYQSLENFMDQLLGTHQTEQQLA
                     EYFGNQRCIVVSGNSEEQTKVADAFGEALSEALDRLLPEGENIIAVMGGTTMAVVAEQ
                     LSNLENKKRHNLFVPARGGIGEAITVQANSVSARMAAKANGNHRALYVPEQLSLATYN
                     SLLNEPSIQEVLNLISEANCVIHSIGRALHMAARRKMTEKELVMLKQANAVAESFGYF
                     FNEKGEVVYKVPRIGIELQDLEKVPIIMAIAGGKSKAKAIRAYMKNAPKQTWLITDEA
                     AANEILKGVTL"
     gene            5452..6453
                     /gene="gap"
                     /locus_tag="IV334_002264"
     CDS             5452..6453
                     /gene="gap"
                     /locus_tag="IV334_002264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HAP8944315.1"
                     /translation="MTVKVGINGFGRIGRLAFRRIQDVDGIEVVAINDLTDAKMLAHL
                     LKYDTTQGRFNGTVEVHEGSFNVNGKEVKVLANRNPEELPWGELGVDIVLECTGFFTS
                     KSAAEKHLTAGAKRVVISAPGGNDVPTIVYNTNHETLTGKETVISGASCTTNCLAPMA
                     KTLNDKFGVVEGLMTTIHAYTGDQMTLDGPHPKGDFRRARAAAANIVPNSTGAAKAIG
                     LVIPELNGKLDGAAQRVPVPTGSLTELVTVLEKEVTVDEINAAMKEASNESYGYNTDE
                     IVSSDIVGMTYGSLFDATQTKVMTVGDKQLVKTVAWYDNEMSYTAQLVRTLEYFANL"
     gene            6563..7753
                     /locus_tag="IV334_002265"
     CDS             6563..7753
                     /locus_tag="IV334_002265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005228679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HAP8944316.1"
                     /translation="MAKKTVKDIDLKDKKVLVRVDFNVPLKDGVITDDTRIKAALPTI
                     NYVLEQGGKAILFSHLGRVKTEEDKEGKSLAPVAKRLGELLGKEVTFVPETRGEQLEE
                     AIRNMNDGDVVVFENTRFEDIDGKKESKNDTELGKYWASLGDVFVNDAFGTAHRAHAS
                     NVGIASTGIPTVAGFLMEKEIKFIGEAVEEPKRPMIAILGGAKVSDKIGVIENLIPKA
                     DKILIGGGMTYTFYEAKGIKIGNSLVEADKVELAKELIEKAGDKLVLPIDNVCAPEFS
                     NDVETQVIEGDIPDGLMALDIGPASVKLFADTLQGAKTVVWNGPMGVFEMSNFAKGTI
                     GVCEAIANLEGATTIIGGGDSAAAAEQLGFADKFTHISTGGGASLELLEGKELPGLAA
                     INDK"
     gene            7857..8612
                     /locus_tag="IV334_002266"
     CDS             7857..8612
                     /locus_tag="IV334_002266"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HAP8944317.1"
                     /translation="MRKPIIAGNWKMNKTASEAKEFAEAVKNKIPSNDKVDSVIGSPA
                     LFLQELMTSAEGTELKISAQNCYWENSGAFTGETSPAALADLGVHYVIIGHSERREYF
                     HETDEDINKKAKAIFANGMTPILCCGESLETYEAGKTAEWIEGQITAGLADLSAEQVS
                     NMVIAYEPIWAIGTGKSADAQIADEICGVVRQTVEKLYGKEVSEAVRIQYGGSVKPEN
                     IAEYMAKENVDGALVGGASLQADSFLSLLDAVK"
     gene            8728..10026
                     /gene="eno"
                     /locus_tag="IV334_002267"
     CDS             8728..10026
                     /gene="eno"
                     /locus_tag="IV334_002267"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HAP8944318.1"
                     /translation="MSIITDVYAREILDSRGNPTIEVEVYTESGAFGRGMVPSGASTG
                     EYEAVELRDGDKARYGGKGVTKAVDNVNNIIAEAIIGYDVRDQMAIDKAMIALDGTPN
                     KGKLGANAILGVSIAVARAAADYLEVPLYHYLGGFNTKVLPTPMMNIINGGSHADNSI
                     DFQEFMIMPVGAPTFKEALRMGAEVFHALASILKARGLATSVGDEGGFAPNLGSNEEG
                     FEVIIEAIEKAGYVPGKDVVLAMDAASSEFYDKEKGVYVLADSGEGEKTTDEMIKFYE
                     ELVSKYPIISIEDGLDENDWDGFKKLTDVLGDKVQLVGDDLFVTNTQKLSEGIEKGIA
                     NSILIKVNQIGTLTETFEAIEMAKEAGYTAVVSHRSGETEDSTISDIAVATNAGQIKT
                     GSLSRTDRIAKYNQLLRIEDQLGEVAEYKGLKSFYNLKNK"
BASE COUNT         3565 a         1886 c         2111 g         2631 t
ORIGIN      
        1 tttttgtcac accttctttt ttgtatataa aaatcccaag taaatacata aaaaataaaa
       61 aaattgaaac ttttagcgca ccttgtacgt catattaagt gaggcagaaa ggagtggaga
      121 atgattttta ctggatataa gaaaaaacgg cagatcgaaa aataccgtca cctgctgaat
      181 ggactcatcg aaactgatta tcaaaaaatg ttccgtatca cgatgaatta cgtacatgat
      241 aaagaagagg ccttagatgt gatgcaagat agttttcata aagccctgaa ctattttgaa
      301 aaagaaaaag agatcgagca ttttagtgca tggttttatc ggatattgat ccgcacggca
      361 ttagatagtt ggcgcagaca aaaaaggacg cctgtggact tatttgaaat tcaagatatc
      421 ccccaacagt tacctttaga aacttccgtt gcagagctcc atatgatttt agataaaata
      481 gacagtccgg aaaaagagat tctgatcctc tacttttttg aaggatttcg tttgaaagaa
      541 attgcgacta tcttggatat gaatgaaaac acagttaaaa cgaaaatgta ccgctcattg
      601 aatgctttac gtcaaatcct agaatgaagg agagaagagt gccatgtatt cccaaaaaga
      661 aaatttaaaa aaactaaaga aaacttataa agaacaagca attcccaaag ggcttaaaga
      721 ggagatccgc cagcgtttta ttatcgaaga gcaatcattt ataaaagcaa agcggagaaa
      781 gagacgtctc caagggttcg gagtatcttt ggctatcgta atgattacaa caagtagcct
      841 tctttttaat agtcaagttc gaagctttgc agaagattta cccatcttag gatcagtcat
      901 tgaattgatc ttaggcgaac gctttactga tcgatctgaa aaaatcgata ttcaggtacc
      961 gaagatcaat acacaaaatg agaaagaaaa taaaacaatt catggattaa atcaaaaata
     1021 ctttcgcgaa ggacaagcag attttgaaaa agctgaaaaa gaatatggaa attttgaaac
     1081 ggatcattac caagttttgg gagattatca gaaaatcgta gacgataacc ggtttctcgt
     1141 gattgaaagg cagatcaccc aaacagcagc agatagccat gttgaaaaaa gatacgacac
     1201 aatcgacaag aaaaacagtg tccagctctc acttccactt ttattcaaag atgacaccta
     1261 tctatctgtt ttgacaaaag aagtcaaacg gcagatggcc gaacaagtca aggaagatcc
     1321 atctaagtac tattggactg agcaagatat tcaagaaggg acgatagaaa aaccaacact
     1381 tgttacgcct acacgaagtt tttatttgaa caaagagcat caattagttt tgactttttc
     1441 tcaatacgaa attgctcctg gttatatggg tacaccagaa tttgtcattc ctaagtccgt
     1501 gacaaaaacg attttggcat cggaagatta tttggatcat tagacgagaa gtagttgcga
     1561 ctagcagttt caattttcaa gtaagtacaa gtaaataata agaagagaaa aagagaaaga
     1621 agagatatag tgaaaagaaa ttatttagtt atcagtatta ttgctttatc cgtgcttttg
     1681 tttttcgaaa ttacttcggt ggcctccttg atcaccgaag taaaaggaca aacacactct
     1741 tcggagcatt cgtcacaatc tttcaaacaa tcagaacaat caaccattaa aagccaatca
     1801 actgaaacag ctaacgtcaa agaagccatt caagaatcag cggaaaaggt acaaagtcag
     1861 tcatcacaat caacacaaga cagtacaaaa acgagccgag atccggctgt caaaagcgtc
     1921 gcaatcagct ttgatgatgg accaggagcg accacaacac cgcagcttct gcgaatctta
     1981 aaagaaaaaa acgtgcatgc cacttttttc gtcttaggag aaaatacggc gcagcatcct
     2041 gaaatcgtga agcagacagc tgaagcaggg cacgagattg gcaatcacac atacgatcat
     2101 cagaatctgg ccactctttc tgcccagtcg atgacggagg aagtgacgaa agcagacact
     2161 gagatcaaaa aagtcactgg caaaaccccc acttttgttc gtccgcccta tggttctgtc
     2221 acgaatgtag gagcaacgat catccagcga ccgatcatcg aatggtctgt tgattcagaa
     2281 gattggaaga caagaaatcc tgatttgatt ttgcagaaga tccaagcaac agtatacgat
     2341 ggggcaatta ttttattcca tgatatctat cctgaaacga ttcgtgccgt tcctcaagtg
     2401 atcgattatc tgaaagagca agggtatcgc atcacgacag ttggggattt attagggcat
     2461 ccaacggctg tcgagaacta ttatggcaga aacgatcatc ggccagttca gtaagtgaga
     2521 caagtatctt taataaataa cacattttaa taagtagttg aacacataac taccttactt
     2581 gcaaaggctt aacaatatct atacaggtac aacaaaaaac tgtccaccaa ttttctttgg
     2641 cggacagttt tcacttaaaa ataaagaagc aactattcgt aaattatgct aaaatagcag
     2701 tagaacgcgt cctgagggaa tcgaaccccc gtctcaagaa ccggaatctt acgtgatatc
     2761 cactacacta aggacgcaag cactgggata attgtaacta aacagataaa aaattacaag
     2821 gggattggac aaaaaaatga aatttgagca aaatttttca caaaaacagc agcagacaca
     2881 gaaactagcc atgacgcaac agttgcaaca gtccatccaa gttttacaat ataacagtga
     2941 ggagctgttg gcattcgttg agaatcaagc aatggaaaat ccattggtgg aagttgttga
     3001 gccggaatgg cagccagatt acataaaagc ttcttcatct tcttatgaag gagaagagac
     3061 aaattaccta aatcagattc ctgatacaaa agggtcttta tttgattcat tgatcgaaca
     3121 agtccatttg aattaccgag atactttttt gaggaaaatc gttctttatc ttgtagaata
     3181 catcgatttg aacggatttt tgacgattag tttagaagaa gccggaaaag agattggagc
     3241 tacgcctatc caaatgctcg atgcgttgac tctgattcag caattagaac cagctggagt
     3301 aggtgcacgt agtttgcaag aatgtctcat gcttcaaaca gaacgagatg attatgcgcc
     3361 ggaattagct tatatcgtgt tggaagaatg ttttgaggaa ctggtggagc gaaaatggaa
     3421 agaaatcgca caacgttttt ctgttgatct acatgctgtt cagcagatat tcgactattt
     3481 acaaacgttg agtccaagcc ctggccggat tttcgaccgt agcagcgaat tgtacattat
     3541 tcctgatgtc cgggtattag tggacgacga taaaaatgta caagttatct caaaccgaaa
     3601 aaatcagcca aatatacgct ttcaagagag ttacttcaaa caaatgtcac aacaagcaga
     3661 taaagaaact gaaaattatt taaaagaacg gaaacaggaa ttcgaatggt tgaagaaaac
     3721 gatcctacaa cgtggggata caattttgcg ggttgctcaa gtcattgttt cccggcaaaa
     3781 agctttcttt atcgacaagg aacgtccaat caagcctctt acgttaaagg aagttgcaac
     3841 tgagatagat gtgcatgaat ccacggtcag tcgtgcagtc aacgggaaat atttggaaac
     3901 agacttcggt gtatttgaat taaaaaaatt tttcactaca cgaattccta cgaatagtac
     3961 agaacagaca gaagaccttt cagcagatac agcgaagaaa aagctgcagg aattagtcga
     4021 tcaagaagac aaaaacaagc cgttgtctga ccagaaattg gttgaattgc tgaaaaaaga
     4081 tgagatagcg atctcaagac gaacagtagc aaaatatcgt gatttattag gaatcccgag
     4141 ttcatcaaaa agaaaacgtt atgataacta atcagtcaaa agactagaaa aagtatttca
     4201 ctttttctag tctttttagt tgaaaaacga gagcaaaact gttagactta cagtcgtgag
     4261 gttgagattt ccctttattg atgtgaaatt cgaatagtta atttcttttt ttatcgtata
     4321 tgggtcgttt taagtctaca ttggacgtaa agtgtccaac taaggagatc gctatgctgg
     4381 atgaaataaa aatgattgaa tctgttgcac cagatattat cgaagtgttg caagaacgct
     4441 ataaaatttt acgaaatatc tattggatgc agccgattgg acgtcgaagt ttatcggaaa
     4501 gtatggggat cacagaacga gtattgcgaa cagaaacaga tcttttgaaa aatctgaatt
     4561 tgatcgacac ctcaaaaagc ggtatgacgc tgacaactaa gggcgaagaa gtttatcaaa
     4621 gccttgaaaa cttcatggat cagcttttag gtacgcatca aaccgaacaa cagttggcag
     4681 aatattttgg taaccaacgc tgtatcgttg tttcaggaaa cagcgaagag cagaccaaag
     4741 tggctgatgc attcggcgaa gcgctgagcg aagccctaga ccgactactt ccagaaggcg
     4801 agaatatcat cgctgtgatg ggaggaacta ctatggctgt ggtagccgaa caattgtcta
     4861 atctggaaaa taagaaacgg cataacctgt ttgtccctgc aagaggcggg attggtgaag
     4921 cgataactgt acaggcaaat tctgtcagtg caagaatggc tgctaaagca aacggtaacc
     4981 accgtgcact ttatgttccg gaacaattaa gtctggcaac ttacaattct ttactcaatg
     5041 agccatctat ccaagaagtc ttaaacttga ttagtgaagc gaattgtgtt attcatagta
     5101 ttgggcgtgc attacacatg gcagcacgtc gtaagatgac tgaaaaagaa ttagtcatgc
     5161 tgaaacaagc aaatgctgtt gcggaatctt ttggctactt tttcaatgaa aaaggtgaag
     5221 tcgtttataa ggtccctaga atcggcatag aattgcaaga tttggaaaaa gtcccgatca
     5281 tcatggcaat cgcgggcgga aaatcaaaag ccaaagcaat tcgagcttat atgaaaaatg
     5341 caccaaaaca aacgtggctc atcactgatg aagctgctgc aaatgagatt ttaaaagggg
     5401 taacccttta aaataaatat ttttttgatt ttcataagga ggaaatctta aatgacagtt
     5461 aaagtaggta ttaatggttt tggacgtatt ggacgtctag cattccgtcg tatccaagat
     5521 gtagatggaa tcgaagtagt agcaatcaac gacttgacag atgctaaaat gttggcacac
     5581 ttgttaaaat acgacacaac tcaaggacgt ttcaacggaa cagttgaagt tcatgaaggt
     5641 tcattcaacg taaacggaaa agaagttaaa gtattagcta accgcaaccc agaagaatta
     5701 ccatggggcg aactaggcgt agatatcgtt ctagaatgta ctggtttctt cacttctaaa
     5761 tcagcagctg aaaaacattt aacagctggt gctaaacgcg ttgttatctc tgctcctggc
     5821 ggaaacgatg ttccaacaat cgtttataac acaaaccacg aaacattaac tggtaaagaa
     5881 acagttatct caggtgcttc atgtactact aactgtttag ctcctatggc taaaacattg
     5941 aacgacaaat ttggtgttgt tgaaggatta atgacaacta tccacgctta cacaggtgac
     6001 caaatgactc tagacggacc tcatcctaaa ggtgacttcc gccgtgcacg cgctgctgct
     6061 gcaaacatcg ttcctaactc aacaggtgct gctaaagcta tcggtttagt aattcctgaa
     6121 ttgaacggta aattagacgg agctgctcaa cgtgttcctg taccaacagg ttcattaaca
     6181 gaattagtaa cagttcttga aaaagaagta actgttgacg aaatcaatgc agcaatgaaa
     6241 gaagcttcaa acgaatctta cggatacaac acagacgaaa tcgtttcttc agatatcgtt
     6301 ggtatgactt acggttcatt atttgatgct acacaaacta aagtaatgac agttggcgac
     6361 aaacaattag ttaaaactgt tgcttggtac gacaacgaaa tgtcatacac tgcacaatta
     6421 gtacgtactt tagaatactt cgctaactta taagatagac aaacgtacaa gtgtaaatta
     6481 tagacatgta catttaaaag cggggaagca atcgcgcttc ttccgctttt tttgataata
     6541 agtttattca ggaggaaata aaatggctaa gaaaacagtg aaagatatcg atttaaaaga
     6601 caaaaaagtt cttgtccgtg ttgacttcaa cgttcctttg aaagacggcg tgatcactga
     6661 cgacactcgt atcaaagcag cattgccaac aatcaactac gttctagaac aaggcggaaa
     6721 agcaatcctt ttctctcacc ttggacgtgt aaaaacagaa gaagataaag aaggaaaatc
     6781 tttagctcca gttgcaaaac gtctaggcga attattaggt aaagaagtaa cattcgtacc
     6841 tgaaactcgc ggagaacaat tagaagaagc aatccgcaac atgaatgacg gcgacgttgt
     6901 cgtattcgaa aatacacgat ttgaagatat tgacggtaaa aaagaaagca aaaatgatac
     6961 agaactaggt aaatactggg cttcattagg cgatgtattt gttaacgatg ctttcggtac
     7021 agctcaccgt gcacatgctt ctaacgtagg tatcgcttca actggtatcc caacagttgc
     7081 tggattcttg atggaaaaag aaatcaagtt catcggtgaa gcagtagaag aaccaaaacg
     7141 accaatgatt gcaatccttg gtggcgcaaa agtttctgat aaaatcggcg taatcgaaaa
     7201 cttgattcct aaagcagata aaatcttgat cggtggcgga atgacttaca cattctacga
     7261 agcaaaagga atcaagatcg gtaactctct agtagaagct gataaagtgg aactagcaaa
     7321 agaattaatc gaaaaagcag gcgacaaact tgtcttgcca atcgacaatg tctgtgcacc
     7381 agaattttca aatgatgttg aaacacaagt aatcgaagga gacattcctg atggtttgat
     7441 ggctttagac atcggcccag catcagtaaa attatttgct gatacattac aaggtgcaaa
     7501 aacagttgtt tggaacggac ctatgggtgt atttgaaatg agtaactttg ctaaaggtac
     7561 aatcggtgtt tgtgaagcaa tcgctaactt agaaggcgca acaacaatca tcggtggcgg
     7621 agactcagcc gcagcagcag aacaattagg atttgctgac aaattcacac acatctcaac
     7681 aggtggcggc gcaagcttgg aattgcttga aggtaaagaa ttaccaggac tagcagcaat
     7741 caacgataaa taaacgacca agtttgctca attctgacga gtaatcgatc aaaaggtgaa
     7801 gggagttttc atgactccct agccattttt caataaatta aggagtgtgt tcctcaatgc
     7861 gtaaacctat tatcgcaggt aactggaaaa tgaacaaaac tgcatcagaa gcaaaagaat
     7921 ttgctgaagc agtaaaaaac aaaatccctt caaatgacaa agttgattca gtcatcggat
     7981 ctcctgcatt attcttacaa gaattaatga catcagctga aggtacagaa ctaaaaatct
     8041 cagcacaaaa ctgctactgg gaaaactcag gtgcgttcac tggtgaaact tcaccagcag
     8101 ctcttgctga tctaggtgtt cattatgtga tcatcggaca ttcagaacgt cgtgaatatt
     8161 tccatgaaac agacgaagac atcaataaaa aagcgaaagc tatctttgca aatggtatga
     8221 caccaatcct atgctgtggc gaatctttag aaacctatga agcaggcaaa actgctgaat
     8281 ggatcgaagg acaaattaca gctggtttag ctgatctttc agctgaacaa gtgtcaaaca
     8341 tggttattgc ttatgaacca atctgggcaa tcggtactgg taaatcagct gacgcacaaa
     8401 tcgctgacga aatttgtgga gttgtacgtc aaacagttga aaaactttat ggaaaagaag
     8461 tatcagaagc agttcgtatc caatacggcg gttctgtaaa accagaaaat attgctgaat
     8521 acatggcaaa agaaaatgtt gacggtgctt tagtcggtgg cgcaagcttg caagcagatt
     8581 cattcttgtc attgttagac gctgtaaaat aattgttgaa aatttgcgaa aaatgcaaga
     8641 aatgtttgca ctaatgagca aaattaacta aaatcgtata tgagggaaat cccttaggaa
     8701 aaaacaaact caaaggagag acaaaacatg tcaattatta ctgatgttta cgctcgcgag
     8761 atcttagact cacgtggtaa cccaacaatc gaagtagaag tatacacaga aagcggagct
     8821 tttggccgcg gaatggttcc ttcaggtgct tcaactggtg aatacgaagc agttgaatta
     8881 cgtgacggag acaaagctcg ttatggcggt aaaggtgtaa ctaaagctgt cgacaacgta
     8941 aacaacatca ttgctgaagc aatcattggc tacgatgttc gtgaccaaat ggctatcgac
     9001 aaagctatga tcgctttaga cggaactcct aacaaaggta aattaggtgc gaacgctatt
     9061 ttaggtgttt ctatcgctgt tgcacgtgca gctgctgact acctagaagt acctttatac
     9121 cactacctag gcggattcaa cacaaaagta ttgccaacac caatgatgaa catcatcaac
     9181 ggcggatcac atgctgataa ctcaatcgac ttccaagaat tcatgatcat gcctgttggc
     9241 gctccaacat tcaaagaagc tctacgtatg ggtgctgaag tattccacgc attagcttca
     9301 atcttgaaag ctcgcggatt agcaacttct gttggtgacg aaggtggatt tgctccaaac
     9361 cttggttcaa acgaagaagg ttttgaagta atcatcgaag caatcgaaaa agctggctat
     9421 gtacctggta aagacgttgt tcttgctatg gatgctgctt cttcagaatt ctacgacaaa
     9481 gaaaaaggtg tttacgtact agctgattca ggcgaaggcg aaaaaacaac tgacgaaatg
     9541 atcaaattct acgaagaatt agtttctaaa tatccaatca tctcaatcga agacggatta
     9601 gacgaaaacg actgggacgg attcaagaaa ttaactgacg tattaggcga caaagttcaa
     9661 ttagttggtg acgacttgtt cgtaacaaac actcaaaaat tatctgaagg tatcgaaaaa
     9721 ggaattgcta actcaatcct tatcaaagtg aaccaaatcg gtacattaac agaaactttc
     9781 gaagctatcg aaatggctaa agaagctggc tacacagcag ttgtatctca ccgttctggt
     9841 gaaacagaag attcaacaat ctctgatatc gctgttgcaa caaacgctgg ccaaatcaaa
     9901 actggttctc tatcacgtac tgaccgtatt gctaaataca accaattact acgtatcgaa
     9961 gaccaacttg gtgaagttgc agaatacaaa ggtttgaaat ctttctacaa cttaaaaaac
    10021 aaataattct tttctaatga agatacatga tcgactattg aaagatagtc gagtatgatc
    10081 acttagttaa gtaatcgaac tctctacttg ctgaaatatc ggctctttgt caataaggac
    10141 tgatgagttg tgcaaaatca aatctgagtc agaatgaacc acattctggc tca
//
LOCUS       DACDKM010000100        10086 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20834_2016
            SAMN12640370-rid9891663.denovo.100, whole genome shotgun sequence.
ACCESSION   DACDKM010000100 DACDKM010000000
VERSION     DACDKM010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640370
            Sequence Read Archive: SRR10040891
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10086)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10086)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742835.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742835.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:04:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,012
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 29 of 75
            Pseudo Genes (incomplete)         :: 33 of 75
            Pseudo Genes (internal stop)      :: 19 of 75
            Pseudo Genes (multiple problems)  :: 5 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10086
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640370-rid9891663.denovo.100"
                     /isolate="E20834_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(247..759)
                     /locus_tag="IV334_002268"
     CDS             complement(247..759)
                     /locus_tag="IV334_002268"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HAP8944319.1"
                     /translation="MDLKDYIASIPDYPSKGIVFRDISPLMADGDAYREATKQIVNYA
                     KEKRIDMVVGPEARGFIVGCPVAYELGVGFAPVRKKGKLPRETIEVTYGLEYGTDTLT
                     LHKDAIKPGQRVLICDDLLATGGTIKATIELIEQLGGVVVGCAFLIELMDLHGRDKIK
                     GYDIITLMEY"
     gene            complement(773..3070)
                     /gene="recJ"
                     /locus_tag="IV334_002269"
     CDS             complement(773..3070)
                     /gene="recJ"
                     /locus_tag="IV334_002269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HAP8944320.1"
                     /translation="MRQANFDWKLSETEPSESFLQMIRELKLSPFVGKLLWQRGYHEE
                     KDINRFLHPKEQELHDPYLIHDMDKAISRIQEAVISGEKILVYGDYDADGITSATVMK
                     ETLELLGAEVEVFLPNRFEHGYGPNQSVYQEKIEEGIQLIITVDNGVAGNDAVAYAQQ
                     AGVDVIITDHHELPENLPDAYAIVHPRHPAGHYPFPDLAGVGVAFKVASALLEEPPAE
                     FLDLVAIGTIADLVSLTDENRILVSLGIDAIHHSERIGLQALFEESGVKMRDADETTI
                     GFSIAPRLNAIGRMGDPRPAVSLLATFDEEEASVQAKKLNEINEERKAIVEQITQEAL
                     AMVNKENHIHLLVNRGWHEGVLGIVAGKIMNETGKPTLVLTLKEDGSAKGSGRSIEAL
                     NLFEMLDQMRDLFTYFGGHHAAVGLTMPSENVTILQEKMNQYIVDHQIDLMRGPELRI
                     DEVLLPNEVTVERIDELKLLAPFGTDNPLPQFLFRQVQAENIKRIGANQQHLKFVLSD
                     ASSQLDAVAFGFGTQEEELLNNQVDVVGKLSINEWNGRKKPQLMVSDFSVGGLQVFDW
                     RAKRFREQSRTSEGSLYLAFDSSTLKFVPEIIQEAAIVFEGLDSVKAIIEKRKPNSLV
                     VMDCPDDLADLKEVFRSYSFNRVYLMGISPDEAYLNGVGTREQYAKLFKLIHSQERID
                     IRHKIKAIAQYLRIPEKLLIFMIQVFFELKFVTIENGVLQKVASPESHPLTESRLYQR
                     RLNKIKVEEFLLLSDIPTIKKWLTT"
     gene            complement(3212..3646)
                     /locus_tag="IV334_002270"
     CDS             complement(3212..3646)
                     /locus_tag="IV334_002270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1049 domain-containing protein"
                     /protein_id="HAP8944321.1"
                     /translation="MKKQSNVILGFVLVLIIVLFAVLNNKNVPVSFGFTSFSAPLILV
                     IIGSALIGALIVFLTASATLWQQKKQIKQLTQEMSEYQTELQKKIEEAKEEQQREFRN
                     ERAELEAAYQAELQAKTAQISQLKAPEEQKTQSPKQSFNYFD"
     gene            complement(3656..4444)
                     /locus_tag="IV334_002271"
     CDS             complement(3656..4444)
                     /locus_tag="IV334_002271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAP8944322.1"
                     /translation="MNLEDKVVLVTGSSGGLGAQICYEAAKQGAIVISCARRMAFVEG
                     VRDECRRLSGKEAYAFKVDVSNPESVDELYEKVMEEVGRVDILVNNAGFGIFEDFLTF
                     DLGKAYDMFEVNILGMMVLTQKFAIDMAERRQGHIINIASMAGKMATAKSTVYSATKF
                     AVLGFSNALRLELKPLGVAVTTVNPGPIETNFFDKADPSGSYLEKVGQIVLEPAKLAK
                     MIVRNMRHPKREINQPFILEVASKFYTLFPTIGDYLASGIFNKK"
     gene            complement(4441..5382)
                     /locus_tag="IV334_002272"
     CDS             complement(4441..5382)
                     /locus_tag="IV334_002272"
                     /EC_number="3.1.26.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Z"
                     /protein_id="HAP8944323.1"
                     /translation="MELQFLGTGAGVPAKHRNVTSIALKLLDERNEVWLFDCGEGTQM
                     QILRTTIRPRKIGKIFITHLHGDHIFGLPGLISSRSFQGGDTPLEIYGPKGIEEYIKV
                     SLGISQTRLSYPLKFIELNETAPIFTDQQFSVYTKKLNHGIDSFGYRVVEHDHKGELQ
                     VDRLKELGIPAGPLYGKLKQGETIQLEDGRTINGKDFVGPDKKGRIVTILGDTRKTHN
                     SVVLAENSDILVHESTFNKDEARMAHNYFHSTTHQAAEVAKEAQAKRLLLTHISARYL
                     GKAALELEKEAQEVFENTTIMKDFDSIEIPFREEEEA"
     gene            complement(5559..6146)
                     /locus_tag="IV334_002273"
     CDS             complement(5559..6146)
                     /locus_tag="IV334_002273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1269 domain-containing protein"
                     /protein_id="HAP8944324.1"
                     /translation="MSKRVIILNFQVESQAYQAFSEIKKLHAVRSIKGEQMAVVSHDP
                     NGTHQFKIEDFIDFTGNNKSSTGGLIGMLVGVLGGPLGILLGWFAGGMIGATQDAKEI
                     KDAQTVFDFVGNKIGEGATGLILIADEEDNRPLNQLVMMELGGEITRLDYEDVEKDVH
                     DAKEAENHAKEAAQKTWKDKHPQTSNDNESSGSSN"
     gene            complement(6367..7680)
                     /gene="obgE"
                     /locus_tag="IV334_002274"
     CDS             complement(6367..7680)
                     /gene="obgE"
                     /locus_tag="IV334_002274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003126661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HAP8944325.1"
                     /translation="MSMFLDQVTIDVKAGKGGDGMVAFRREKYVPDGGPAGGDGGRGG
                     DVILIVDEGLRTLMDFRFNRHFKAQPGENGMSKGMHGRGSEDTYVKVPQGTTVRDAET
                     GALLGDLIENGQTLVVAKGGRGGRGNIRFASPRNPAPEIAENGEPGQERKIELELKVL
                     ADVGLVGFPSVGKSTLLSVISSARPKIGAYHFTTLVPNLGMVTTSDGRSFAAADLPGL
                     IEGASQGVGLGTQFLRHIERTRVILHVIDMSGMEGRDPYEDYLAINKELSTYNLRLLE
                     RPQIIVANKMDMPDAPENLVKFKEQLNKEKEDEFADDIPVFPISGVTRQGLDALLNAT
                     ADLLEVTPEFPLYEEELEEETVHYGFNPEGPEFQIDRDSDATWILSGEKIEKLFQMTN
                     FDHDETVMRFARQLRGMGVDEALRARGAKDGDLVRIGEFEFEFVE"
     gene            7968..8978
                     /gene="gap"
                     /locus_tag="IV334_002275"
     CDS             7968..8978
                     /gene="gap"
                     /locus_tag="IV334_002275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HAP8944326.1"
                     /translation="MTVKVGINGFGRIGRLAFRRIKEVSDDIEVVAINDLTSPTMLAQ
                     LLQFDSTHGTYPGTVTATEDSIVVDGEATRVYAEPDASKIPWAKENGVDIVLECTGFY
                     TSEEKAKAHLDAGVKRVVISAPAGAMKTIVYNVNDDTLDENDKIISAGSCTTNCLAPM
                     AYFLNNEFGIEVGTMTTVHAYTSTQMLLDGPVKGGNLRAARSAADNTIPHSTGAAKAI
                     GLVIPELQGKLQGHAQRVPVVDGSLTELVSVLKTKVTADQVNEAIKKHTVDNPSFGYD
                     DRQIVSSDVIGTTQGSIFDPTQTEVTTAGDFQLVKTVAWYDNEYGFTCQMIRLLEKFA
                     NL"
     gene            complement(9063..9998)
                     /locus_tag="IV334_002276"
     CDS             complement(9063..9998)
                     /locus_tag="IV334_002276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAP8944327.1"
                     /translation="MKLWMKILLGVALALIVALVFAGNYFYNYAVVPSEKDFLDEDTP
                     GTTQTSKETDAHKWFSSKENRSDWTITSKDGLKLSAIYLPAETKSEKTALVAHGYMGN
                     AETMTNYAKMFHDMGYNVLVPDARGHGKSEGDYIGFGWPERKDYVQWINKVLENNGKS
                     QEIVLYGVSMGAATVMMTSGEKLPDNVKAIIEDCGYSSVHDELAYQLDDMFSLPAFPL
                     MQVTSLVTKVRAGYFFGEANAVEQLKKNQRPMLFIHGDEDTFVPFEMLDKVYRATKGP
                     KEKYVVKGAEHAEAYKTDPEKYQQVVQQFLSQYVH"
BASE COUNT         2778 a         2298 c         1746 g         3264 t
ORIGIN      
        1 ttttacgggg gtgtgacaaa aatcggtcac atcctttttt tcgaataaac ggtgttctaa
       61 agccagtttt tcggtattaa gtcaaattgc ggaaaaagtg attttttaaa cttctactgg
      121 tctttgcttg tttccattta taaaaaaata gcgaccctca aaagtcagat agagaatctg
      181 acttttgagg gtcgctttaa aatcgcttct ttaagaataa catcaagaat agcttctttt
      241 ttctgcttaa tattccatta atgtaatgat gtcatatcct ttgattttgt cgcgtccatg
      301 gagatccatc aattcgatca aaaatgcgca gcctacgact acaccgccta attgttcgat
      361 cagttcgatc gtcgctttga ttgttcctcc agtagctagc aaatcgtcac aaatcagcac
      421 acgttgacct ggtttaatgg catccttatg caatgtcaat gtatctgttc catattctaa
      481 tccataagtc acttcgatgg tttctcgagg taacttccct tttttgcgga ctggggcaaa
      541 accgactcct aactcataag caacaggaca tcctacgatg aatccacggg cttctggccc
      601 aactaccata tcaattcgtt tttcttttgc ataatttacg atttgtttag tagcttcccg
      661 ataagcgtca ccatctgcca tcagtgggga aatatctcga aacacaattc cttttgacgg
      721 atagtcggga atactggcaa tataatcttt taaatccact ctttgtttcc tcctaggttg
      781 ttaaccattt ctttatggta ggaatatcgc tcaaaagcaa aaattcctca actttgattt
      841 tattgagccg tcgctggtat aatctgctct cagtcaaagg atgactttct gggctagcga
      901 ctttttgtaa aacaccgttc tctattgtaa caaatttcaa ttcaaaaaac acctgtatca
      961 taaaaattaa taatttttca gggatacgca aatattgagc tattgctttt attttatgac
     1021 gtatatcaat tcgttcttga gaatggatca atttaaacaa ttttgcgtac tgttctcttg
     1081 tgcctacccc attcaaatag gcctcatcag gtgaaatccc catcagataa acacgattga
     1141 atgaataaga ccgaaaaact tctttcagat ccgctaaatc atctggacaa tccataacaa
     1201 ctagagagtt tggttttctt ttttcaatta ttgcctttac agaatctagt ccttcgaaaa
     1261 caatcgcagc ttcctggata atctcaggca cgaatttcaa cgtcgaagaa tcaaatgcta
     1321 aataaaggct accttccgac gttcggctct gttcacggaa acgttttgcc cgccaatcaa
     1381 atacttgcag accacccaca gaaaaatcag ataccatcag ctgcggtttc ttacgcccgt
     1441 tccattcatt gatcgaaagc tttcctacca catctacctg attattcagc agttcttctt
     1501 cttgagtacc aaaaccaaaa gcgaccgcat caagctgact cgatgcatcg gaaaggacaa
     1561 atttcagatg ctgttggttt gctccgattc ttttgatatt ttctgcttgg acttgtcgaa
     1621 ataaaaactg aggcaaagga ttatccgtac caaatggcgc caatagtttc agctcatcaa
     1681 ttcgctctac tgttacttca tttggcagca atacttcatc aattctcaac tcaggccctc
     1741 tcatcaagtc aatttgatga tcgactatat attggttcat cttctcctgt agtatagtga
     1801 cattttcact tggcatcgtt aaaccgactg ctgcatgatg tcctccgaag taagtaaata
     1861 aatctcgcat ctggtcaagc atttcaaaaa gattcaaggc ttcaatacta cgtccagatc
     1921 ctttagctga tccatcttct tttaaagtaa ggacaagcgt tggtttaccc gtttcattca
     1981 ttattttccc agcaacaatg cccaaaacgc cttcatgcca tcctcggttt accaatagat
     2041 ggatatgatt ctccttattg accatcgcta atgcttcttg tgttatctgc tctacgattg
     2101 cttttctttc ttcattgatc tcattcaatt tttttgcttg aacggatgct tcctcttcat
     2161 caaacgtcgc aagtaacgag acggcaggac gtggatcgcc catccgaccg attgcattta
     2221 gtctgggagc aatcgaaaaa ccgatcgttg tttcgtctgc gtccctcatt tttacgccgc
     2281 tttcttcaaa tagcgcctga agtcctatcc gttctgaatg atgaatggca tcgattccta
     2341 atgaaacaag gattcgattc tcatcagtca atgaaactaa atcggcgatc gttcctatcg
     2401 ctactaaatc caaaaattct gcaggcggtt cttccaataa tgcagaagct actttgaatg
     2461 ctactccgac tcctgccaaa tctggaaatg gatagtgccc cgctggatga cgagggtgaa
     2521 caatcgcata agcgtctggt aaattctcag gtagttcgtg atgatctgta atgatcacgt
     2581 ctacaccagc ttgctgagcg tatgctaccg catcatttcc tgctaccccg ttatcaacag
     2641 tgatgatcaa ctggattcct tcttcgatct tttcttggta taccgattgg tttggtccat
     2701 acccatgctc aaatcgattt ggcaggaata cttctacttc tgcaccaagt aattccagtg
     2761 tttctttcat taccgttgca ctggtgatcc catctgcgtc ataatcaccg taaacgagga
     2821 ttttttctcc actgattaca gcttcttgga ttcggctgat tgctttatcc atatcgtgta
     2881 tcagatacgg atcatgtaat tcttgttcct ttggatgtaa aaatcggttg atatcctttt
     2941 cctcatggta acctctttgc cacagtaact taccgacaaa tggagataat ttcagttcac
     3001 ggatcatttg gagaaaggat tcagaaggct ctgtctcgct taacttccaa tcaaaatttg
     3061 cttgtctcac tttctcactc ctaacttagt tattatagca aaggaaatca gcaaaaaaaa
     3121 gcagaaagaa agaataaaaa cagatcagat ccgctagaca gatgttcagc aggacctgat
     3181 cttttgtacg accatttatg aaatatccct attagtcaaa ataattgaag ctttgtttgg
     3241 gcgattgagt tttttgttct tccggcgctt tcaattgact gatttgtgcg gttttcgctt
     3301 gtaactccgc ctgataagca gcttccaact ccgcacgttc gtttctaaac tcgcgttgct
     3361 gttcttcttt tgcttcttca attttctttt gaagttctgt ttgatattct gacatttcct
     3421 gagtcagctg tttgatttgc tttttttgct gccatagtgt tgccgaagca gtcaaaaata
     3481 caatcagtgc accaatcagc gcagaaccga tgatcactaa gatcaatggc gcagaaaagg
     3541 aagtgaagcc aaaacttaca ggaacatttt tattgttcaa tacagcaaaa agaacaataa
     3601 tcaaaactaa aacaaatcca agaatcacat tgctttgctt tttcaattcc ttcacctact
     3661 ttttgttaaa aattccgctg gctaaataat caccaatcgt tggaaacagc gtataaaatt
     3721 ttgatgcaac ttctaaaata aacggttgat tgatttcccg ttttggatgt ctcatattgc
     3781 ggacgatcat ttttgccaac ttggcaggtt ctaaaacgat ttgtccgact ttttccaaat
     3841 agcttccgct tggatcagcc ttatcgaaga aatttgtctc gattggacct ggattcactg
     3901 tcgtaacagc tacgccaagt ggtttcaatt ctaatcgcaa agcgttggag aagccaagga
     3961 ctgcaaattt cgtggcagaa taaaccgttg attttgcagt tgccattttt ccagccatag
     4021 aggcgatatt aatgatatga ccttgtcttc tttctgccat atcgatcgca aatttttgtg
     4081 tcaaaaccat cattcctaag atgttgactt caaacatatc gtatgctttt ccaaggtcga
     4141 atgtaaggaa gtcttcgaaa atcccaaaac ccgcattgtt taccagaata tcgacgcgcc
     4201 cgacttcttc catcaccttt tcatataatt cgtccacgct ttcaggattg ctgacgtcta
     4261 ccttgaaagc ataagcctct ttgccgctca agcgtctgca ctcgtctctg acaccttcta
     4321 caaatgccat tcttcgtgcg caacttataa cgattgctcc ttgctttgct gcttcataac
     4381 agatttgtgc gcccagaccg cctgaactac ctgttactaa aacaacttta tcttctaaat
     4441 tcatgcctct tcctcctcgc gaaatggaat ctcaatgcta tcaaaatctt tcataatcgt
     4501 cgtgttttca aacacttctt gtgcttcttt ttcaagctct aaagctgctt ttcctaaata
     4561 tctcgcactg atatgagtaa gtagcagccg ttttgcctgc gcttcttttg caacttcagc
     4621 agcttgatga gtcgtggaat ggaaatagtt gtgcgccatt cttgcttcat ctttattaaa
     4681 tgtgctttca tgtaccagaa tatcgctatt ttccgcaagc acaacggaat tatgtgtttt
     4741 tctcgtatcg cctaaaatag tcacgattcg tcctttttta tctggaccta cgaaatcttt
     4801 tccattgatc gtccgcccat cttctagttg gatcgtttca ccttgcttca gtttgccgta
     4861 cagtggtcca gctggaatac ctaattcttt tagacgatcc acctgcaatt ctcctttatg
     4921 atcatgttca accacacgat aaccaaaact gtcgatccca tgatttagtt tttttgtata
     4981 aacagaaaat tgctgatcag taaaaatcgg ggctgtttca tttagctcaa tgaatttcag
     5041 cggataactt aaacgcgttt gcgaaatacc taaagagact tttatgtatt cctctatccc
     5101 ttttggcccg taaatctcta atggcgtatc cccaccttga aatgaccggc tgctgatgag
     5161 cccgggaagt ccaaaaatat gatcgccgtg taaatgtgta atgaaaattt taccgatttt
     5221 tctcggacga atagtcgttc gcaaaatctg catttgcgtg ccttcgccac agtcgaacaa
     5281 ccaaacttca ttgcgctcgt ctaaaagttt caatgctata cttgtcacat ttctatgttt
     5341 tgcaggaaca cctgcacctg ttcctaaaaa ttgtaattcc ataatctacc tgactttctt
     5401 taagttcctc tttaatttta gggtaaaaag aagggataat caaccgtaag tcgagaatgg
     5461 gttcaaatta aaaataacgt tctaaagcga tagcgttttc tttgaaaatt actataaaaa
     5521 cagcagcgaa cccgtttgag atcctctgct gttttttatt aatttgaaga tccagaactt
     5581 tcattgtcgt tggaagtttg cggatgtttg tccttccaag tcttttgtgc agcttctttc
     5641 gcatgatttt cagcttcttt cgcatcatgc acatcttttt cgacatcttc ataatccaag
     5701 cgagtaattt ctccgcctaa ttccatcatt accaattgat tcagtgggcg attgtcctct
     5761 tcatcagcaa tcaagatcag tcctgtagct ccttcaccga ttttatttcc tacaaagtcg
     5821 aacacggtct gggcatcttt tatttctttg gcatcttgtg tcgcaccgat cattcctccg
     5881 gcaaaccatc ctaataaaat ccctagcgga ccacctaata caccaaccaa cattccgatt
     5941 aatccacccg tagaagattt gttattaccg gtaaagtcga taaaatcttc tattttgaat
     6001 tgatgtgtcc cattaggatc atggctgaca actgccatct gttctccttt aatcgaacga
     6061 acagcatgta actttttgat ttctgaaaat gcctgatatg cctggctttc tacttgaaaa
     6121 tttaaaatga ttactcgttt gctcattaaa aacgcccctt tcataccttc ttgttattta
     6181 ttttaccttt aattgaaaaa taagaaaaga aaagcactct ttttttccaa taaaataggg
     6241 ctgtgacaaa agtcgtatca cagctccttt ttccgaataa acggcgttct tgaagctgat
     6301 cacttctgtc acaacctccc ttaaatctga atctatattt tttggtattt gtttttattt
     6361 gcttgcctat tcgacaaatt caaattcaaa ctcaccgata cgtacaaggt cgccatcttt
     6421 cgcaccgcgt gcccgaagag cttcatccac acccatacca cgtaattgac gagcaaaacg
     6481 catcactgtt tcatcatgat cgaaattcgt catttggaat aatttttcga tcttctcacc
     6541 agataagatc cacgttgcat ctgaatcacg atcgatttgg aactcaggac cttctggatt
     6601 gaatccatag tgtacagttt cttcttccag ttcttcttcg tacaatggga attctggcgt
     6661 tacttctaac aaatctgctg tagcattcaa taacgcgtcc aatccttgtc gagtcactcc
     6721 agaaattgga aatactggga tgtcatcagc aaattcatct tctttttcct tgttcagctg
     6781 ttctttgaat ttcactagat tttccggtgc atccggcata tccattttgt ttgccacgat
     6841 gatttgcgga cgctctagca agcgtaagtt ataagtagat aattctttgt tgatggcaag
     6901 atagtcttca taaggatctc gtccttccat cccactcata tcgatcacat gtaaaattac
     6961 tcgtgttctt tcgatatgac gaaggaattg tgtacccagc ccaacacctt gcgaagctcc
     7021 ttcaatcaag cctggcaagt ctgctgcggc aaaacttctg ccatcacttg tagtaaccat
     7081 tcctagatta ggtacgagcg tcgtgaaatg ataagcgccg atttttggac gagcagatga
     7141 aataacggat agtaaagtcg atttcccgac agatggaaaa cctacaagtc caacgtctgc
     7201 caatactttc aattctagtt cgatttttcg ctcttgtcct ggttcaccgt tttcagcaat
     7261 ttccggtgca ggattccgag gagaggcaaa acggatattc cctcgtccac cgcgtccacc
     7321 ttttgccaca actagtgttt gaccattttc aattaaatct cctaaaagag caccagtctc
     7381 cgcatctcgg acagtggttc cttgtggtac tttaacatag gtatcttctg aaccacgacc
     7441 gtgcatccct ttactcatcc cattttcacc tggctgcgct ttgaaatggc gattgaaccg
     7501 aaaatccatc aatgtacgta acccttcatc gacgatcaag atgacatcgc cgccacgacc
     7561 accgtctccg ccagccggtc cgccgtcagg aacgtatttt tcgcgacgaa atgcaaccat
     7621 tccgtctccg cccttgccag ctttcacatc gatcgttact tgatctaaaa acatggacat
     7681 aattaagtcc tcctattatt catttattta tttgactgcc tgaatttcca caataaacaa
     7741 aagtcttact tattttatcg gtgaaatctg attttgtcta tcatttcagc aaaaagattg
     7801 tttaccttct tcttttttta tcatttggta aggtttccaa ccttttttct gctaccaagt
     7861 ataatttttt gacaaaatat tctttgcgta gcagaataac agtgtattaa ttgtattaat
     7921 tgaggatgcc gtttcacagc acccctaata aaggaggaac ttttgatatg acagtaaaag
     7981 taggtattaa cggatttggg cgtattggcc gtctggcttt ccgccgcatc aaagaagtct
     8041 cagatgatat tgaagtagta gcaatcaacg acttaacaag tccaactatg ttagcacaat
     8101 tattgcaatt tgactctact catggtactt atcctggcac agttactgca acagaagact
     8161 caatcgtagt ggatggcgaa gcaactcgtg tttatgcaga acccgacgca agcaaaatcc
     8221 cttgggcaaa agaaaacggt gtagatatcg tactagaatg tactggtttt tatacatctg
     8281 aagaaaaagc aaaagcgcat ttagatgctg gtgtcaaacg agtagttatt tctgccccag
     8341 ctggtgctat gaaaaccatc gtctataacg tcaatgatga tacattagat gaaaacgaca
     8401 aaatcatctc agcaggttca tgtacaacaa actgtttagc acctatggct tatttcttaa
     8461 ataatgaatt tgggatcgaa gttggtacaa tgacaacagt tcatgcctac acttccacac
     8521 aaatgctctt agatggacct gtaaaaggcg gtaatttgcg tgctgctcgt tcagctgctg
     8581 acaatactat cccacattct actggtgctg cgaaagcaat cggcttagtt atccctgagc
     8641 tacaaggaaa attacaagga cacgcacaac gtgttccagt cgttgatggt tcattaactg
     8701 aattggtttc agtattgaaa acaaaagtaa ctgctgatca agtaaacgaa gcaatcaaaa
     8761 aacacacagt tgacaatcct tcattcgggt atgatgaccg tcaaatcgtt tctagcgatg
     8821 tcattggaac aactcaaggt tcgatctttg acccaacgca aacagaagta acaactgctg
     8881 gtgatttcca acttgttaaa actgttgcat ggtatgacaa tgagtatgga tttacttgcc
     8941 aaatgattcg cttactagaa aaattcgcta acttgtaatt tgatagatca atcgataaaa
     9001 gaagcctttg actggatcca gtcaaaggct tctttctttt catcccctac ttttaaactc
     9061 gttcaatgaa catattggct taaaaattgt tggactactt gctgatattt ttcaggatct
     9121 gtcttgtatg cttccgcatg ctcggctcct tttacaacat acttttcttt tggtcccttc
     9181 gttgcacgat agaccttatc taacatttca aatggtacaa atgtatcttc gtctccatgt
     9241 ataaagagca ttgggcgctg gtttttcttc aattgttcca ctgcatttgc ttcaccgaaa
     9301 aagtagccag cacggacttt tgtcactaga ctcgttactt gcataagcgg aaatgctgga
     9361 agactaaaca tatcgtctag ctgataagca agctcatcat gaacggaaga ataaccgcaa
     9421 tcttcaatga ttgctttcac attatctggt agtttttctc cgctagtcat cataactgtt
     9481 gctgctccca tgctgacacc gtatagaacg atttcttgcg attttccatt gttttctagc
     9541 actttgttta tccactgtac atagtcttta cgctctggcc atccaaatcc aatgtaatcc
     9601 ccttcgcttt ttccatgtcc tcttgcatca ggaacaagga cattgtaacc catatcatga
     9661 aacatctttg cgtaattggt catcgtttca gcgtttccca tatagccatg agccacaaga
     9721 gctgtttttt cacttttcgt ttcagcagga agataaatcg ctgataattt cagaccgtct
     9781 tttgaagtga tggtccaatc gcttcggttt tcttttgaac taaaccattt atgtgcatcc
     9841 gtttctttgg atgtctgtgt tgtaccaggt gtatcttcgt ctaaaaagtc tttctcagaa
     9901 ggcacaacag cataattata aaaataattc cctgcaaaca ctaacgcaac aatcagagct
     9961 aaagcgacgc ctaaaaggat tttcatccaa agtttcataa atcatccatc tcatttctct
    10021 tttttcatat aaccttgaat tagtataatc aaagaatctc tactatgcaa tgttcatttt
    10081 tataac
//
LOCUS       DACDKM010000101         9823 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20834_2016
            SAMN12640370-rid9891663.denovo.101, whole genome shotgun sequence.
ACCESSION   DACDKM010000101 DACDKM010000000
VERSION     DACDKM010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640370
            Sequence Read Archive: SRR10040891
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9823)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9823)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742835.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742835.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:04:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,012
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 29 of 75
            Pseudo Genes (incomplete)         :: 33 of 75
            Pseudo Genes (internal stop)      :: 19 of 75
            Pseudo Genes (multiple problems)  :: 5 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9823
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640370-rid9891663.denovo.101"
                     /isolate="E20834_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(<1..195)
                     /locus_tag="IV334_002277"
     CDS             complement(<1..195)
                     /locus_tag="IV334_002277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8944328.1"
                     /translation="MLKISYKPSTDSKEMKKEYETVNDFLQGQYLEVPPLQDHFVVTT
                     VTLDGKEIEMPDQTISGLFNY"
     gene            complement(211..825)
                     /locus_tag="IV334_002278"
     CDS             complement(211..825)
                     /locus_tag="IV334_002278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HAP8944329.1"
                     /translation="MFERSPSELTERENYKLLIGSVIPRPVAVVTTKSAKDIVNIAPF
                     SYFNIVSSNRPILSLAIQRKQDAVKDTAKNILETREAVIHILDEDNVEEVNKTAANLP
                     NEESELYLTDLTLTDSVIISVPALTEAKVRFETSLYEHVEIRNKNKVTADLFLLEVQK
                     YHLNETVYDDKTGRIDPRKLNAVSRLAGNDYAAIGEIFTIARPE"
     gene            complement(972..1655)
                     /locus_tag="IV334_002279"
     CDS             complement(972..1655)
                     /locus_tag="IV334_002279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HAP8944330.1"
                     /translation="MDTLEQIKGGVVVSCQALENEPLHSSFIMGRMALAAKEGGAVGI
                     RANTTEDINEIKRIVDLPVIGIIKRDYDGSEVFITATEKEVEELLETQAEIIALDATS
                     RKRPTEKTTAELVQMIHEHGRLAMADISTFEEALQAQEDGFDLLSTTLAGYTEYSRKT
                     QTPDFDLLNQIIEQVDIPVIMEGHTDSPEQVEKAYELGAFSVVVGSIITRPQLITKRY
                     VEAASKANN"
     gene            complement(1764..2222)
                     /locus_tag="IV334_002280"
     CDS             complement(1764..2222)
                     /locus_tag="IV334_002280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH domain-containing protein"
                     /protein_id="HAP8944331.1"
                     /translation="MYQQVEEYVKKFQEKYPSYQSEKLGFFSFGDTEKMADELSELVV
                     KKIKTGTSSGYDLYKQENEPLPKVGQLDVVLNGKNEPVCIIKNINVTILPFSEVTKEH
                     AWKEGEGDRTLAYWRKVHAEFFKHAYKEALNIEFTEEKLVVYEEFEVIFF"
     gene            complement(2306..4069)
                     /locus_tag="IV334_002281"
     CDS             complement(2306..4069)
                     /locus_tag="IV334_002281"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HAP8944332.1"
                     /translation="MSTVLHVLDAINSPADVKKLSVEEMKQLAEEMRQKILQKDSDVG
                     GHVGPNLGVVELTIAFHYVFDSPKDKIVWDVSHQSYGHKLLTGRKNGFEKGHYREVSG
                     YSNQHESKHDYFTVGHTSTSISLATGLAKARDLKKESGNVVAFIGDGSLSGGLAFEGL
                     NNGGVLQSNLIVLVNDNEMSIDENHGSLYKNLAELRQTNGKSSNNLFKAMGFDYRYVD
                     NGNDLETVIRLFEEVKDIDHPIVLHVHTEKGRGYQPAIDNKMKYHWHVPFDLSTGEAK
                     QVTSGETFNMIIMDYLDKKMQTSTAPIAAVNAAIPGAFGLKTFQEKHPDHYFDVGIAE
                     QHSITFASALASQGVRPVVFQSSTFLQRAYDQLSHDLAINENPVIILVGGGTISASDR
                     THQGTFDIPYLSSIPNIKYLAPATKEDMISMLDWAFEQTAQPVAIRIPTHGVEHGTAI
                     ANDYSIPAYQTVNNGGKVAILALGGFLELGKETVKAFRKKGIEATLINPGTITENDVE
                     TLDKLKETHELVITLEDGSLSGGFGENISRYYGDSSMNVLNFGAEKEFTDSVPVGQLY
                     ERYHLTPKQIVEDSLRVLNIQ"
     gene            complement(4290..5750)
                     /locus_tag="IV334_002282"
     CDS             complement(4290..5750)
                     /locus_tag="IV334_002282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8944333.1"
                     /translation="MLENYIERNIFRKVYLCEQLFEFQEIDIEQTAISLRVTTPTILH
                     DLESLAECLEYCIKEQVREKHKYKLVFKHGIALSELTQFLYGQSYFLKFLSYYFNGIF
                     TSTELADLEFISLSKVYTIKKIVLDFFKENDYLKNKEIIIPEFDSRNILLALVRYVNW
                     EGYENKNPYVEEACNELIDYVETHFFKRRYSEDEKSFIIRGIEIAIGRKAFPLGLKEE
                     DKKSAETKPLFQLVCQGLEEQKGILDLQEEDVYYIFSLFNSRNYTNENMELLRKDFEV
                     VYKNFVLNDLSLNELILQIISIAEIDDAENFIFKQSFLQFVRTLWADGQVFLPERIYL
                     LSEKEQLLYGKIVEILEEWKKKNQIQLRWNENFIRKFVKSLSLINSEQAGRQEIEIFV
                     VSESSVKQFFYRTQFHLYAEEGIAEINPILFRSLEELPDECLCAKKRVVLCDAASYQN
                     GLETEKTKIYPISLKGANIHLYHIAQELHLLKKHIS"
     gene            complement(5958..6884)
                     /gene="glsA"
                     /locus_tag="IV334_002283"
     CDS             complement(5958..6884)
                     /gene="glsA"
                     /locus_tag="IV334_002283"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase A"
                     /protein_id="HAP8944334.1"
                     /translation="MEVKELEQLIGKNFHYYQTGQVADYIPALAEVDPKQLGMAIYDL
                     DTKQLIEGGDADVRFAIESMSKVPVLLLAIKDNGIEKVFQTINTEPTGFAFNSIMNME
                     INHRKHPMNPFVNAGAIATTSLIAGKDADEKFERILSFMKEICDDPNITLNEEIYHSE
                     SRTGDINRSLAYYMKGNQMIEGDVPEILDAYFRQCSLNVTATGIAKLAAVLANKGIAP
                     WNGKQIITEESATIVKSIMTTAGLYDESGEFSVHVGVPAKSGVGGGLMAAVPNRYGIG
                     VFSPALDPFGNSAAGIQLLKDVVKELDADIFE"
     gene            complement(6907..8334)
                     /locus_tag="IV334_002284"
     CDS             complement(6907..8334)
                     /locus_tag="IV334_002284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAP8944335.1"
                     /translation="MQQSKTKIPAKKLTFFGFLAMTISMVVSLYEYPTFATSGFSLVF
                     FLLLGGLLWFIPVALCAAEMATVKGWEKGGVYTWVSRTLGKRFGFAAIFFQWFEITVG
                     YLTMLYFLTGALSYATGIGAIQNNKYLKLAILLIIFWAILLSQLKGTKYTSLIARIGF
                     VAGILLPALVLFGLGIHYVASGAPLQTILSMKTLIPDFSKLPTLVVFVSFILAYMGVE
                     TSASHANEMENPKKDYPLAMFVLVILAIILDTFGGLTVATTVPQHGLSLNTGVIQSLE
                     FLLRHIDPNLAWIAKILAILVCLGVIGEIASWVTSPSKALHVAAEEGLLPEYFAKENT
                     HGVPAHLMIANGIVATIWAAVFTLSGGGNNLSFLAAMSLTVVIYLMLYFMFFAGYLKL
                     VFKDDSLERAYQVPGGKVVKVIVACLGLLTSLFAFCISFVPPASIAAGQHGIYETILI
                     VSYLIVLVIPFTIYACRHKFQHAEK"
     gene            complement(8365..9579)
                     /locus_tag="IV334_002285"
     CDS             complement(8365..9579)
                     /locus_tag="IV334_002285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HAP8944336.1"
                     /translation="MNTLPINTGDTAFMILCTAMVCLMTPGLAFFYGGLARKNNILTI
                     MSQSFLSVGITTIMWVFGGFGLAFGRDIGGVIGNPADFFLMRHVTSFPNMFHGATIPF
                     LMFFLYQLMFIVITVPLMTGAFAGRLNLKGYIILVIFWNLLIYFPVCHWIWGGGFLDQ
                     MGFRDFAGGAVIHTTAGFGSLACILTLGQRKIPLGERSQHSSLMAAAIGTGLLWFGWF
                     GFNSGGALRADEQAVNAFGSTFIALAFAMITWLIIAKVKGNGFDFVDVLTGSVAGLAA
                     ITPCAGYVEAKSAMLIGIIAGIVCHAAVDFRKKKQWDDALDVWGVHGMGGFTGTILIG
                     IFASNSHLLTNPSSWYFLGIQVIGVIITAVYAYVLTTVILKSAMHFTTITTTKEEQEE
                     GLDFFFFGNKLN"
BASE COUNT         2915 a         2041 c         1628 g         3239 t
ORIGIN      
        1 gtaattaaac aatccgctaa tcgtttgatc tggcatttct atttccttac catctaaagt
       61 aacagtagta acgacaaaat ggtcttgcaa tggcggaact tctaagtact gcccttgcag
      121 aaaatcattc actgtttcat attctttttt catttctttt gaatctgtcg atggtttata
      181 gcttattttt aacataacta gtgctccttt ttattcggga cgtgcaatcg tgaaaatttc
      241 tcctattgct gcataatcat ttcctgccaa acggcttaca gcattcaatt ttcttggatc
      301 gatccttcct gtcttatcat catagactgt ttcatttaaa tgatatttct gtacttctag
      361 caaaaataaa tcagctgtta ctttattttt attccgtatc tcgacatgtt catataagga
      421 tgtttcaaaa cgaacttttg cttctgtcaa tgctggtact gaaatgatta ctgaatcagt
      481 taaagtcaag tcagtcaggt acagttcgct ttcctcatta ggcagattgg ctgctgtttt
      541 atttacttct tctacgttgt cctcatcaag gatatggata acagcttctc ttgtctcaag
      601 aatattcttt gctgtgtctt ttactgcatc ttgttttctc tggatcgcca aggataagat
      661 gggtcgattc gaactgacga tattgaagta actgaagggt gcgatattca ctatatcttt
      721 tgctgattta gtcgttacta ctgctactgg tctagggatg actgaaccaa tcagcaactt
      781 gtaattttca cgttctgtca attctgaagg actccgttca aacatggttt cgccttcttt
      841 ctataaaatt cgttaaaaag cagatcttga agacaaaatg aaaagtaatg cttttttcat
      901 cttcttagaa tctgctttaa actatctatc aatttttctt ttttgtcaag aatctgtgcc
      961 gtgcgtgttt tttagttatt tgctttactt gcggcttcga catatctttt agtgatcaat
     1021 tgcggacggg tgataataga tccaactacg actgaaaaag cacctaattc atatgctttc
     1081 tctacttgtt caggagagtc agtatgtcct tccataatta ctgggatatc gacttgttct
     1141 atgatttgat tcaataaatc aaaatcaggt gtctgcgtct ttcgtgaata ttctgtatag
     1201 cctgctaaag tcgtagacaa aagatcaaaa ccatcttctt gcgcttgaag cgcttcctca
     1261 aaagtcgaaa tatctgccat agctaaccga ccgtgttcgt gaatcatttg gacaagttca
     1321 gcagttgttt tttctgtcgg acgtttacga gaagttgcat ccagtgcaat gatttctgct
     1381 tgcgtctcca aaagttcttc cacttctttt tctgtcgcag taataaacac ttctgaccca
     1441 tcgtaatctc ttttgataat ccctattacc ggtaaatcaa cgattcgctt gatttcattg
     1501 atatcttctg tcgtatttgc tcgaatacca actgctccgc cttcttttgc agccaaagcc
     1561 attctcccca taataaaaga actgtgtagc ggctcgtttt ccaaagcttg acaagaaacg
     1621 acgactccgc ctttgatttg ttccagtgta tccacaatat ctcctccagt aatgttatat
     1681 aaaaatacag cttcatataa aaatgtaacc cttttctatt caggagacaa taatgagcta
     1741 tttctttcag cagacctttc ttactaaaag aaaattactt caaattcttc gtatacgacc
     1801 agcttctctt ctgtaaattc tatgttcaac gcttctttat aagcatgttt aaaaaactca
     1861 gcatggactt tccgccaata agctaatgtc cgatcccctt ctccttcttt ccaagcatgc
     1921 tctttcgtga cttcactaaa aggcaagatg gtcacattta tatttttgat gatacaaact
     1981 ggttcatttt ttccattcag gaccacgtct aattgaccaa cttttggtaa aggctcattc
     2041 tcttgtttat acagatcgta accacttgat gtaccagtct ttatcttttt tacaactagt
     2101 tcgctcaact cgtctgccat tttttctgta tccccaaatg aaaagaaacc taatttttct
     2161 gattgataac taggatattt ttcttggaac tttttgacat attcttctac ttgctgatac
     2221 ataacatcca cccctttgct ttttataatg aaaaaaccat ccagatacta gtataaacaa
     2281 ttatctggat ggtgagcata atgaattatt gaatattcaa gacacgcaga ctatcttcaa
     2341 cgatttgttt tggtgttaaa tgataacgtt cataaagctg ccctacagga acgctatccg
     2401 tgaattcttt ctctgcacca aaatttaata cgttcattga agaatcacca taataacgac
     2461 tgatattttc gccaaatcct cctgacaaac taccatcttc taatgtgatg actaattcgt
     2521 gtgtttcttt taatttatct aatgtttcta cgtcattttc tgtgatcgtt cctggattga
     2581 tcaacgttgc ttcgattcct ttttttctaa aagcttttac tgtttctttt cctaattcaa
     2641 gaaaaccgcc taaagctaaa attgctactt ttcctccatt atttactgtt tgataggctg
     2701 gaatactata atcatttgcg atagctgtac catgttctac accatgtgta ggaattcgga
     2761 ttgctacagg ctgcgctgtt tgttcgaacg cccaatctag catagagatc atatcctctt
     2821 ttgttgctgg agcaagatat ttgatgttcg ggatacttga taagtaagga atgtcaaatg
     2881 ttccttggtg tgttcggtca ctagctgaga tcgttccgcc accgactaaa ataatcactg
     2941 gattctcgtt gatcgctaaa tcatgtgata attgatcata tgcccgttgc aagaaagtac
     3001 ttgattggaa gacgactgga cgtactccct gagaagccaa agcagaagcg aaagtgatag
     3061 agtgctgttc agcgatcccc acgtcaaaat aatgatctgg atgtttttct tggaatgttt
     3121 tcagtccaaa ggcgcctggt atcgctgcat tgactgcggc aataggagct gtacttgttt
     3181 gcattttctt atccaaataa tccatgatga tcatattaaa tgtttctcca gaagttactt
     3241 gctttgcctc cccggtggac agatcaaatg gtacatgcca atgatatttc attttattat
     3301 cgattgccgg ctgataaccg cgaccttttt ccgtatggac gtgaagtaca atcggatgat
     3361 caatatcttt tacttcttca aataaacgga tcaccgtttc cagatcgttt ccattatcca
     3421 cgtaacgata atcaaatccc attgctttaa ataggttatt gctgctttta ccgttggttt
     3481 gacgaagctc agctaagttt ttatataaac tgccatgatt ctcatcaata gacatctcat
     3541 tatcgtttac taatacgatt agattggatt gtaaaacacc accattgttc aatccttcaa
     3601 aagctaagcc accacttagg gagccgtcac cgataaatgc taccacattt cctgattctt
     3661 tcttcagatc tctcgcttta gctaaacctg ttgctaagct gatagatgta gatgtgtgcc
     3721 ccactgtaaa atagtcatgt ttactttcat gttggttgga atatccgctc acttcacggt
     3781 aatgtccttt ttcaaaacca ttttttcgac cagttaaaag cttatgacca taagactgat
     3841 gggaaacatc ccaaacgatt ttatcctttg gtgaatcaaa tacataatga aacgcaatcg
     3901 ttaattctac tacacctaga ttcggaccaa cgtgcccacc tacatcgctg tccttttgca
     3961 agattttttg gcgcatctct tctgccaact gtttcatttc ctcaacagat aattttttga
     4021 catctgcggg gctattgatt gcatccaata catgtaatac tgtactcata atacattctc
     4081 tcctccgtac tatcttcttt taatgaaaat agatttgtta cactttatct attctactac
     4141 ttagagttga ctctaagtca atagcatttt aaaaaaattt cagagaaagc ttgttatata
     4201 agtagatctt cgcacgaatc aggactcaaa taaaaacctt ctacagacaa ttaaaaagct
     4261 ttgtagaagg tctcctaatt aaggtctcat caagaaatat gtttttttaa taggtgaagt
     4321 tcctgagcaa tatgatacaa atggatgttt gcacctttta atgagattgg atagatcttt
     4381 gtcttttcag tttctaatcc attttgatag ctagcagcat cgcacaaaac cactcttttt
     4441 ttcgcacata gacattcatc aggcaattct tctaatgaac gaaataaaat aggatttatt
     4501 tcagcgatgc cttcttccgc atacaaatga aattgggttc gataaaaaaa ttgtttgact
     4561 gaactttctg aaacaacaaa aatttctatt tcctgtctcc ccgcttgttc actgttgatc
     4621 aaagaaagac ttttgacgaa ttttcgaatg aaattttcat tccatcttag ttggatttga
     4681 tttttctttt tccactcttc caatatttct acaatttttc cgtacagaag ttgttctttt
     4741 tctgatagaa gatagatcct ttccggtaaa aatacttgtc catctgccca caacgtacgg
     4801 acaaattgta aaaaggattg cttgaaaata aaattttctg catcgtcgat ttcagcgata
     4861 ctaataatct gtaaaatcaa ttcattcagt gataggtcat tgagaacaaa atttttataa
     4921 acaacttcga aatcttttct taacagctcc atgttctcat tggtataatt tctactgttg
     4981 aacaaagaaa aaatataata gacatcttct tcttgtaagt ctagaattcc tttttgctct
     5041 tccaatcctt gacagactaa ttgaaataac ggcttcgttt ctgcgctttt cttatcttct
     5101 tctttcagtc caaggggaaa tgcctttctt ccgattgcga tctctattcc tcgtatgata
     5161 aaggactttt catcttccga atatcttctt ttaaaaaaat gagtctctac ataatcaatc
     5221 aactcgttac aagcttcttc tacatacgga tttttatttt catagccttc ccaattgaca
     5281 taacgtacta aagctagcaa gatgttccgc gagtcaaatt ctgggataat gatttcttta
     5341 tttttcaaat aatcattttc tttaaaaaaa tccaatacga tttttttgat tgtatatact
     5401 ttgctcaaag aaataaattc aagatcagcc agttctgtac tcgtgaatat accattaaaa
     5461 taataactta aaaactttaa aaaataagat tgaccgtaca gaaactgtgt gagctcagaa
     5521 agagcaatac catgtttgaa tacgagttta tacttatgct tctctcttac ttgttctttt
     5581 atacaatatt ctagacactc ggctagactt tctagatcgt gaaggatcgt tggtgtagtc
     5641 acccttagag aaatagctgt ttgttcgatg tctatttctt ggaattcaaa taattgttca
     5701 cacagataca cttttctaaa tatatttcgc tcgatgtaat tttccagcat tcctgataca
     5761 cgcctccagt ttttcttatg attttattta atcatatctg taaatcgtat ttttgtttcc
     5821 tttttattta gaaaagccct ttcaaaaacc aataaaaaaa gaaactggga caaaagtcaa
     5881 acaagctcac aatttcagcc tcattgtagg tagctttgag ccgtttggac ttttacccca
     5941 gcctctttca agaatggtta ttcaaagatg tcagcgtcta gttctttgac tacatctttc
     6001 aacaattgga taccagctgc gctgttacca aatggatcta gtgccgggct gaagacacct
     6061 atgccgtaac gatttggcac agctgccatc aacccgccgc ctacaccgct ttttgcaggt
     6121 acaccaacat ggacggaaaa ctctcctgat tcatcataca aaccagcagt tgtcataatt
     6181 gatttgacga ttgtagcact ttcttcagta atgatttgtt tgccattcca aggagcaatt
     6241 cctttatttg ccagcacagc agctaatttt gctatgcctg tagcggtgac attgagggag
     6301 cattgtctga aataagcatc gagtatctcc ggtacgtctc cttcgatcat ttgattacct
     6361 ttcatatagt aagcaagaga gcgattgata tcacctgttc ttgattcaga atgatagatt
     6421 tcttcattca atgtgatatt aggatcatca cagatctctt tcataaatga caagattcgc
     6481 tcaaattttt catctgcatc tttaccggca atcaacgagg ttgttgcaat cgctcctgca
     6541 ttaacaaaag gattcattgg atgtttgcga tgattgattt ccatattcat gatagaattg
     6601 aaggcaaaac cggttggttc cgtgttgatt gtttgaaaga ctttttctat tccgttgtct
     6661 ttgattgcta ataacaatac gggaactttt gacatgcttt cgatggcaaa gcgcacatcg
     6721 gcgtctcctc cctcaatcag ttgttttgta tccaaatcat aaatcgccat acctaattgt
     6781 ttgggatcca cttcagctaa tgctggaatg taatccgcca cttgtcctgt ttgataatag
     6841 tggaaatttt ttccaatcaa ttgttctaat tcttttactt ccatttgaat aaccagcttt
     6901 cattttttat ttttctgcat gctggaattt atgacggcat gcgtagatcg taaatggaat
     6961 aactaagacg ataagataag aaacaatcag aatcgtttca taaatcccat gctgtccagc
     7021 tgcgatacta gcaggtggaa caaatgagat acaaaaagca aaaagtgaag tcaaaagacc
     7081 taaacaagca acgattactt ttacaacttt tccgcctgga acctgatagg ctctttcaag
     7141 actgtcatct ttaaaaacca gcttcaaata gcccgcgaaa aacataaagt acaacatcag
     7201 gtagattaca acagtcaatg acatcgctgc aagaaatgaa agattattcc ccccgccgct
     7261 taatgtaaag acggctgccc agattgttgc aacgatccca tttgcgatca tcaaatgagc
     7321 aggaacaccg tgtgtgtttt cttttgcaaa atattcagga agtaatcctt cttctgctgc
     7381 tacgtgaagt gcttttgaag gagaggttac ccaagaagca atttctccga tgacacctaa
     7441 acaaacaaga atcgcaagaa ttttcgcaat ccatgcaaga tttggatcaa tgtgtcttaa
     7501 taggaattct aacgattgaa tcacccctgt attaagagac aaaccatgtt gaggtactgt
     7561 agtagcgacg gtcaatccgc caaaagtatc taaaatgatt gccagaataa ccaatacaaa
     7621 cattgctaat ggataatcct ttttagggtt ttccatttca tttgcatgac tggcagatgt
     7681 ttctacgccc atatatgcta aaataaagga aacaaaaaca accagcgtag gcaatttcga
     7741 aaaatcagga atcaatgttt tcatagacag tatcgtttgt agtggagcgc ctgaagcgac
     7801 ataatggatc cctaagccaa acaagactaa tgctggcaac aagataccag ctacaaaacc
     7861 gatccgagca atcaatgaag tgtatttggt tccttttaac tgtgagagta aaatagccca
     7921 gaaaataatc aataggattg ccaatttcag atatttattg ttttgtatag caccaatacc
     7981 cgttgcatag gataatgcac cagttaagaa atacagcatg gtcaagtagc caactgttat
     8041 ctcaaaccat tggaaaaaga ttgcggcaaa accgaatcgt tttcccaatg tgcgactgac
     8101 ccaagtatag acaccgcctt tttcccatcc tttcaccgtt gccatctctg ctgcgcacaa
     8161 tgccacaggg ataaaccata gcaaaccgcc taataacaag aaaaatacta agctgaagcc
     8221 tgaggtcgcg aatgtgggat attcatataa tgaaacgacc attgagatgg tcatagccaa
     8281 aaagccaaag aacgttaatt tttttgcagg aattttcgtt ttgctttgtt gcatgaatct
     8341 tcctcttttc tatttgaatt tgtttcaatt gagtttgttg ccaaagaaga aaaagtccaa
     8401 tccttcttct tgttcttctt ttgtagtagt gatcgttgta aagtgcatcg ccgattttaa
     8461 aatcactgtt gtcagcacat aggcatacac ggcagtaatg atgacaccaa tcacttgaat
     8521 ccctaagaag taccagctgc ttggattcgt tagtaaatgg ctgttagatg caaagatccc
     8581 tatcagaatc gtacctgtga atccgcccat gccatgaaca ccccacacat caagtgcatc
     8641 atcccattgt ttctttttac gaaaatcaac agcagcatga cagacgatac ccgcaatgat
     8701 cccaatcagc atggcacttt ttgcttctac ataaccggca caaggcgtaa tggctgccaa
     8761 tccagcaacg gatcctgtta gtacgtcgac aaaatcaaag ccatttcctt ttacttttgc
     8821 aatgatcagc caagtaatca tcgcaaatgc aagagcgatg aatgttgaac caaatgcatt
     8881 gactgcttgt tcatctgctc gaagtgcacc accggaatta aaaccgaacc agccaaacca
     8941 aagcagtcct gtaccgatag cagctgccat aagactgcta tgctgacttc tttctcctaa
     9001 aggaatcttt ctttgaccaa gtgtgaggat acatgctaaa gagccgaatc cagctgtcgt
     9061 gtggatgact gctccaccag cgaaatctct aaatcccatc tgatccaaaa atccgccacc
     9121 ccatatccag tggcaaacag ggaaataaat cagcagattc caaaaaataa caagtataat
     9181 atagcctttt aaattcaatc tccctgcaaa agctcctgtc atcaaaggaa cggtaatcac
     9241 gataaacatc agttggtaaa gaaagaacat taaaaatgga atcgttgcac catgaaacat
     9301 gttggggaag cttgtaacat gtctcatcaa aaagaaatct gccgggtttc caataacacc
     9361 gccgatatcc cgtccaaagg ccaatccgaa tccaccaaat acccacataa tcgttgttat
     9421 cccaacggag agaaaagatt gagacatgat cgttagtata ttattcttcc gtgctaatcc
     9481 gccgtaaaaa aaagctagac caggagtcat caggcaaacc attgctgtac ataaaatcat
     9541 gaatgctgta tctcctgtat tgataggtaa tgtattcatt tttttcgcct cctttctacg
     9601 attacgagta taaaagaagg tgtagttgtt tttttcttct gtttttttga attaatactt
     9661 gcatttataa aagacagaca attaataaaa gcttgatgtg ttgtgctctt tttcgttttt
     9721 cacacaaata aagacaaaaa ataaacggtc ttctttttat agaaaaccgt ttattttcgt
     9781 ctttttttat ttttaaagat attttttcat aaggaataaa gaa
//
LOCUS       DACDKM010000102         9612 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20834_2016
            SAMN12640370-rid9891663.denovo.102, whole genome shotgun sequence.
ACCESSION   DACDKM010000102 DACDKM010000000
VERSION     DACDKM010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640370
            Sequence Read Archive: SRR10040891
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9612)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9612)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742835.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742835.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:04:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,012
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 29 of 75
            Pseudo Genes (incomplete)         :: 33 of 75
            Pseudo Genes (internal stop)      :: 19 of 75
            Pseudo Genes (multiple problems)  :: 5 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9612
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640370-rid9891663.denovo.102"
                     /isolate="E20834_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(226..648)
                     /locus_tag="IV334_002286"
     CDS             complement(226..648)
                     /locus_tag="IV334_002286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HAP8944337.1"
                     /translation="MRNKKVIVRILIVSFLLFGCFKLIKTNEESNGKLINSNLEEVLE
                     LQKTNQSFFVYLARNDCKKCQEVESFLERYDKKFSRNVYRIETRKEPRQEELQTFLQR
                     YHVKSVPSFLVIHDRNVKKIERKTFFFEKTLNQFLPNF"
     gene            complement(638..2812)
                     /locus_tag="IV334_002287"
     CDS             complement(638..2812)
                     /locus_tag="IV334_002287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF853 family protein"
                     /protein_id="HAP8944338.1"
                     /translation="MMNAPKRPTHFEQMFSVDRKRKQEKETPKSRFQQLYFIVTLLGV
                     CLYPFFIVGSIPLAIVFMIDKKETRAHIYDFEYESFLKRRSSLFFIFALGFTLLNLFC
                     FIFVLPRGYLSCYLLFPLNLFSHALQFNGQTIFALLLGGTGAGSCLIAYASFIEKRKV
                     VSKKERQTALLNSKEYKERRKNKFQESQKYTAEYQQAYQVAIVIKDVALRNKQLASLA
                     KVILLGTDEYGLPYVMNFSELNQHVIIPATTGSGKTTVIQLLAEHACKFRIPVILVDG
                     KGAGETLQALKEVAEAYNRPIEVFVDGGDLRYNPVKNGNDIAIRDKLTNLAETESVYY
                     STAAKALLQVTIQLLDEFQKSHKIERNLVWLQHYLLPRNVLHLFADRIQLKNPDLFKE
                     KVVVEKKKTTKKKTKKNEAMDKTERDFADVEQLSEERIVEHDRKTVDLETYYLLLKEN
                     ISLMTEIEQTLFKRLFVRYEHKKNPFYLYATSENLQTNINMLLDSELGELFDTSGGKR
                     ELDIQEVVKEGKIVYISLNGLIYSEFIRTLAQMLVGDINFFASEVYKQAGETANRSKV
                     LVIFDEPASYLNDNLIDLVNKGRGAGVHAAYAPQTLADIDRIDPVLRKQLIGNVNTFF
                     IGKTNDPTEIDYWADLMGTEEDIELTEMTTQEAGYSDMGKVDWTGERGTKRDVDRFKF
                     NPNRIRALRTGEFVVYRTAEDVQEPPRVVYIRKPNLEKQYAK"
     gene            complement(2828..5170)
                     /locus_tag="IV334_002288"
     CDS             complement(2828..5170)
                     /locus_tag="IV334_002288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8944339.1"
                     /translation="MRKKSLKWWTLLLLGGLFFCLQGATVSADQMNDGAGVTETDRSE
                     GENANDKVEVKEVTTDVDQLYKNYKKNSFELMTNDYESMNLKKKLSYNVAGVMKNFVW
                     SGVKMLGEFNASMVEFLFELDIISAIREPIQNLTQKIADNMLSIAGTIGISFSAIMMA
                     IKYAGEARMKQALRVFLMTILIFTGLSVLKDSGTNKNLFDVLFSLDSTVETAFVKVNP
                     VLTPEEETNYETKETEEVTADKKVETAGKLMAARVFYSNVYEPYLLLNYGTTNVDTIR
                     EKKVKYAGKEYDRINLLLDNDLGTKIGEKIHDKVTEYEADTLKNKNIQYYNSWKNTAF
                     ALFYFVVNLIQTVVYFVLSMFRLIIGTMQLLLVPMLPFLLFLGLFLVTMNVFGNYAKV
                     FGILTFLKGMTGFACILFSSFLSYGFQLSSTQDDPWEKILLIIVYLFAPLGIYLFRGF
                     LGSLFTGRMGVRGALAFAGNPFGAEAKLRREAKQRLKERKEARKAQKQKEKENARKRQ
                     ERQRKRGGGSDVRMPQTAKQATRSAFRRESAQQPRHKAPNRMERMAQKARDLHDQAKQ
                     AEEQSQERMQRTAMEKQARKTADLLQRELQGNPSLRERSRKGASRSPYRNQGSAMVVS
                     LANPKQKHKSYGQKHTPRTELNDRKRRQGQTSRRRKEALTPVTKTSSMKTRTVAGQKH
                     AVQKPLRKGNVPRATGSTTPKSVRRPEGVREHMSAVNRVTAQTKAPSTVSKAYSTMKQ
                     TKSPKQAPKTRSEFRATQAKMKRKREKQPIYRREMKQRKR"
     gene            complement(5192..7735)
                     /locus_tag="IV334_002289"
     CDS             complement(5192..7735)
                     /locus_tag="IV334_002289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion protein"
                     /protein_id="HAP8944340.1"
                     /translation="MAFLFLLVVFIIGIWQFKKKKTKLPRLEFPIDAIIRNFVLTKND
                     DVWVGYQLSSQVFPLNDIDFFKNYLQDGEGILGHDEYDYHFINIPRQFNLDTHIEETI
                     RDLVKGECADLGKVYFEQAGKILKDEVQLNEYRTYLFVRFTTEMKVSDPIEYFQLLKH
                     VFKKFIYRITGQRVPISSVLPTYETLEKELYTELSNYKPIERLSAQEIGQIYYYFFHR
                     SEEKLPERQLTPMEICEGVISNEAGYIAIEQLNQTHYSTFLSQTDAPLAMYGSSFIQN
                     LQDSLSFPIDTHIRLRFEHSKSDERKINKMAGRIYEQEKEYETVDGILDSDDVLNFGT
                     ERLMDVKDKLKRKEKRLCYSTVTLVVSASSKEELEERVKDIEFAIESTDFKFYRSVAD
                     QLTLFNQCLIGSTQRFRSYEQVATTGFVADYGLDLKKEVGNKKGFPLGRIVIAKKFKS
                     AKEALALSSKLVWYYPNLTKKSIEGAEHTNGNTLIIGPPGKGKSVLVKYIFLWLMFLG
                     QKILYIDPKNETQKFFLKALEKFGHIPEFVAMYERINFISLSSDESCRGMLDPLLFLP
                     KEEAIQTAKNVLGMLGEVDTNRETASHKKTVIQDAIDVVYTMPGKHSLSQVIEEIRKS
                     DKELAKLILGHSVGLGKILIGNEHSTPIRFDSQINVLGTQGIRIPTQKEIDSGRLNSE
                     QLAGMAIMEVIMKFTYIFSTNKEEDAAIIYDEAKGYEDTAQGSYLIDDSLRKGRANNT
                     DIYLVVQAFKDYDQEDKKELISYKFAFRPKQKEAREKLLSFFDMEVNSANLEMMEQLQ
                     TGTCLFQDHLGRNQPIAIDVLFDSWLLAVSSTENQDEKIQAMLENEKQR"
     gene            complement(7745..8143)
                     /locus_tag="IV334_002290"
     CDS             complement(7745..8143)
                     /locus_tag="IV334_002290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAP8944341.1"
                     /translation="MEYNYTKEFKHPHKIYGIKGQKIPWISNGIRMEQLVIGGVFLFI
                     LLILFLIAYVNQISFLQTLFRKSWLIMIFGIGVLVWTLFSMKWDNKHFFEYLIGRTSY
                     YQKRKQRFEHEMYVPFFHEKVTYSLHKWKE"
     gene            complement(8224..8508)
                     /locus_tag="IV334_002291"
     CDS             complement(8224..8508)
                     /locus_tag="IV334_002291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8944342.1"
                     /translation="MKLFDLKSLFDDVSGQVIWLLAIVAIVLMVGAYATQGFARAAFA
                     IIGVFILIMLVLLLSNAVDIGEWLKNIIFIKDSEENVGVTQMIVPWIQLS"
     gene            complement(8532..9530)
                     /locus_tag="IV334_002292"
     CDS             complement(8532..9530)
                     /locus_tag="IV334_002292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAP8944343.1"
                     /translation="MSNAKFNPQAFQTKPPKQQKEKKQKKRFLFQKKKTEPSDRMIQF
                     SQKSAMSVFSGAFFCLFLLMGVVLLLNFGRVDTLTRLAVKKQVNPETLLTEVNQTLSS
                     TEQLKYEGQHFIERLFTYSPEKRNEWEASVNHYLAQGLEASDLGFVTTATNRVAKDVR
                     FIKLEEIDLTQAMYRLFYEVTFTEGEHWKSSQLSFPVSYQNNVLKVLDGVQFLNLSES
                     KNTNSVNYQENRFYSSGQAVTESEEIKLEAFVKRFFELYVKNDEKLALISNEKGLEGG
                     ELINVQLDHAVKNKEGNYFLQGKYRFYFEKGNEFQSNFSMEVKTSGDSYFVTTLNE"
BASE COUNT         2753 a         2035 c         1492 g         3332 t
ORIGIN      
        1 gatttcaaac aaaatacgtt tattttttct attgatttta gctcaaaatc gattcaattc
       61 gagtgaaatt tgcttcaatt ttcaattggt ttttgttcaa attttgaaac atttttgctc
      121 aaaaaatgag tgaatttgtt tcaaaagaaa aacaaatacg atcgaaaaaa atttgaactc
      181 ataacaattt tttgtttttt tcaacgtatt ctctttcaat tttcactaaa aattaggaag
      241 aaattgattc aatgtctttt caaaaaagaa tgtttttctc tcaatttttt tgacgtttct
      301 atcatgaatc acgagaaaac ttgggacact tttaacatgg taacgttgta aaaaagtttg
      361 aagttcttct tgtcttggtt cttttcttgt ttcaattcga tatacattcc tagagaattt
      421 tttgtcgtat ctttccaaga aagattcaac ttcttggcat tttttacaat catttcttgc
      481 tagatacaca aaaaatgatt gattcgtttt ttgaagttct agtacttctt ctaaattact
      541 attgattagt tttccatttg attcttcgtt agtttttatc agtttaaaac aaccaaataa
      601 gaggaaacta acaataagta tcctaacaat aactttctta tttcgcatat tgtttctcca
      661 aatttggttt ccgaatatag acgactcgcg gtggttcctg gacgtcttca gctgttcgat
      721 acaccacaaa ttcgcctgtt ctcaatgctc gaatgcgatt cggattgaat ttaaagcggt
      781 ctacgtctcg tttcgtgcct ctttctcctg tccaatcaac ctttcccata tcagaataac
      841 ctgcttcttg tgtcgtcatt tccgttaatt caatgtcttc ttctgtcccc attaaatctg
      901 cccaataatc aatttctgtc gggtcatttg tcttcccaat aaagaacgta ttcacattcc
      961 caatcaactg ttttcgaagg acaggatcaa ttctatcaat gtccgcaagc gtttgagggg
     1021 catacgccgc atgcacccca gcgcctcgtc ccttattgac taagtctata aggttatcat
     1081 ttagataaga agctggttcg tcaaatatta ctaggacttt cgagcgattg gctgtttctc
     1141 ctgcttgttt atacacttct gacgcaaaga aattaatatc tcccacaagc atttgagcca
     1201 atgtccgaat aaactcacta taaatcaatc cgtttaacga gatataaaca atctttcctt
     1261 ctttaacaac ttcttgaata tctaattctc tttttcctcc agaagtatca aagagttcac
     1321 caagctcact atccaataac atattgatat tggtctgtaa attctcagaa gtcgcataca
     1381 aataaaacgg gttcttctta tgttcgtaac gaacaaacaa ccgtttaaac aacgtttgct
     1441 cgatttctgt catgagagaa atgttttctt ttaataatag ataatacgtt tctaagtcaa
     1501 cggttttacg atcatgttca acgattcgct cttcacttaa ctgttccaca tcggcaaaat
     1561 cacgttctgt tttgtccatc gcttcattct ttttcgtttt tttcttcgtg gttttcttct
     1621 tttctacgac cactttttct ttaaataaat ctggattttt caactgaata cgatccgcaa
     1681 acaaatgtaa gacgtttcgt ggcaataaat aatgttgtaa ccaaactaga tttctttcaa
     1741 ttttatggct cttttggaac tcgtctaaaa gctgaatcgt tacctgtaat aacgctttgg
     1801 ctgctgtcga ataatacacg ctttctgttt ctgctagatt ggttaattta tctcgaatgg
     1861 caatatcatt gccatttttt acaggattat aacgcaaatc gccaccgtcc acaaagactt
     1921 caatcgggcg attgtatgcc tctgccactt cttttaacgc ttgtaaagtc tctcctgctc
     1981 ctttcccatc gactaagata acggggatac gaaacttaca cgcatgttct gctagtaatt
     2041 ggatcaccgt cgtttttcct gaacctgtag tcgctggaat aatcacatgt tggttcaatt
     2101 cactaaagtt catcacataa gggagcccat actcatctgt tcctaataga atcactttgg
     2161 ctaatgaagc cagttgttta ttccgcaaag caacgtcttt tatgacaatt gctacctggt
     2221 acgcttgctg atactctgct gtatattttt gggactcctg aaatttattc ttccgacgtt
     2281 ccttgtattc tttactattt agcaaagctg tttgccgttc tttctttgaa acaacttttc
     2341 ttttttcaat aaaagacgca tacgcaatca aacaacttcc tgcacctgtc ccacctaaaa
     2401 ggagggcaaa aatcgtttgt ccattaaact gcaacgcatg agaaaacaag tttaatggaa
     2461 ataataagta acacgacaaa taccctctag gaagaacaaa aataaaacaa aatagattta
     2521 atagcgtaaa acctaacgca aaaataaaaa aaagagagct tcttcgtttt aagaagctct
     2581 catactcaaa gtcataaata tgtgcacgtg tctctttttt atctatcata aagacaatcg
     2641 ctaaaggaat actgcctaca ataaaaaatg gatatagaca aacacctaat aaagtgacga
     2701 taaaataaag ttgttgaaac cgactttttg gcgtttcttt ttcttgtttt cttttgcgat
     2761 cgacagaaaa catttgctca aaatgtgtcg gtcgttttgg tgcattcatc atactatcga
     2821 tttcctccta tcgttttcgt tgtttcattt ctcttcgata aatcggttgt ttttctcgtt
     2881 ttcgtttcat ttttgcttga gttgctcgaa actcactccg tgtcttcggt gcttgttttg
     2941 gtgattttgt ttgtttcatg gttgagtatg ctttagatac ggttgaaggc gctttggttt
     3001 gtgctgttac tcgattgact gctgacatat gttcccgaac cccctctgga cgtcgaacag
     3061 atttcggtgt ggtagaacct gttgctcgtg gtacattccc ttttcttagt ggtttttgta
     3121 ccgcatgttt ttgtcctgca actgtccggg ttttcatcga acttgttttt gttacgggag
     3181 taagtgcctc cttccgacga cgacttgttt gtccctgacg acgttttcga tcgttcaatt
     3241 ctgttctagg cgtatgcttc tggccataag acttgtgttt ttgctttgga ttcgctaatg
     3301 acaccaccat tgcagaaccc tgattgcgat acggactacg agacgcacct tttctacttc
     3361 tttctcttag actaggattg ccttgcaatt ctcgttgtaa taaatcggct gtttttcttg
     3421 cctgtttctc cattgctgtt cgttgcatac gttcttgact ttgctcttct gcttgtttcg
     3481 cttgatcatg caagtctctt gctttttgtg ccatacgttc catacgattt ggtgctttat
     3541 gacgaggttg ttgtgcggat tcacgccgaa aagcacttcg tgtggcttgt ttcgccgttt
     3601 gtggcatacg aacgtctgag ccacccccac gttttcgttg acgttcttgt ctcttccgtg
     3661 cgttttcttt ttctttttgt ttctgtgctt ttctcgcttc tttgcgttct ttcaaccgtt
     3721 gtttggcttc ccttctcaat tttgcttctg ccccaaatgg atttcccgca aaagctaacg
     3781 ctcctcgaac acccattctt ccagtaaata gactgcctaa aaagcctcgg aacaaataaa
     3841 ttcccaacgg cgcaaacaaa taaacaatga tcaagagaat tttttcccat ggatcgtctt
     3901 gtgtactact taattggaac ccgtaactaa gaaaagaaga aaaaaggata catgcaaaac
     3961 ctgtcattcc ttttaaaaac gtcaaaatac caaaaacttt ggcatagtta ccaaacacat
     4021 tcattgtgac aagaaacaat cctaaaaaaa gtaagaacgg caacatcggt actaacaata
     4081 gttgcatagt tccaataatc aatcgaaaca tacttaagac aaaataaaca acggtttgaa
     4141 tcagattcac gacaaaataa aacaaggcaa atgctgtatt tttccaacta ttgtaatatt
     4201 gtatgttctt gtttttcagt gtatctgctt catactctgt gactttgtca tgaattttct
     4261 cccctatttt tgtacctaaa tcattgtcca ataaaagatt gattcgatca tattcttttc
     4321 cagcatattt gactttcttc tcacgaattg tgtctacatt ggttgtgcca taatttaaca
     4381 acagatatgg ttcataaaca ttactataaa agacccgagc cgccatcaat ttgcctgccg
     4441 tttccacttt tttatccgct gttacctctt ctgtttcttt tgtttcataa tttgtttctt
     4501 cttcaggtgt taacactgga ttgaccttca cgaacgccgt ttccactgta gaatctaaag
     4561 aaaaaagaac atcaaaaaga ttcttatttg tcccagaatc ttttaatacg cttaacccag
     4621 taaaaataag gatcgtcata agaaagacac ggagtgcttg tttcattcga gcctctccag
     4681 catatttgat tgccatcata attgccgaaa aactgattcc aatagttcct gcaatactta
     4741 acatattgtc cgcaattttt tgtgttaagt tttgaatggg ttctcgaatg gcggaaataa
     4801 tatctaattc aaagagaaat tcaaccattg aagcattgaa ttctcctaac atttttacgc
     4861 cactccaaac aaaatttttc atcactcctg caacattgta cgatagcttt tttttaaggt
     4921 tcattgactc atagtcattg gtcatcaatt caaacgaatt ctttttgtaa tttttataca
     4981 attgatccac atcagtcgtt acttctttta cttctacttt atcgttggca ttctctcctt
     5041 cagaacgatc ggtttcagtg acacccgcac catcattcat ttgatcggca gaaacagtag
     5101 ccccttgtag acagaaaaaa agacccccta ataaaaggag cgtccaccat ttcagagatt
     5161 tttttcgcaa aaaaaccacc tcttttttgt tttatcgttg tttttcattt tctaacattg
     5221 cttgaatttt ttcatcttga ttttcagtag aagaaacagc caataaccaa ctatcaaaca
     5281 acacatcaat ggcaatcggt tgatttcttc ccaaatgatc ctggaataaa caggttcctg
     5341 tttgtaattg ttccatcatt tctaaattgg ctgaattcac ttccatatca aagaaggaca
     5401 atagtttctc tcgtgcttct ttttgtttcg gacgaaacgc aaatttgtaa gaaattaact
     5461 cttttttatc ttcttgatcg taatctttaa acgcttgcac aacaaggtaa atatccgtat
     5521 tattcgcacg tccttttcgc aagctatcat caatcaaata gcttccttgt gcggtatctt
     5581 catacccttt tgcctcgtca taaataatcg cagcgtcttc ttctttgttt gtagaaaaga
     5641 tatacgtaaa cttcataatg acttccataa ttgccattcc tgcaagttgt tcgctattta
     5701 atcgacccga atcaatctct ttttgtgtcg gaatgcgtat tccttgtgtc cctaacacgt
     5761 tgatttgtga atcaaaacgt atcggcgtac tgtgctcatt cccaatgaga atctttccta
     5821 aaccaacgct atgccctaaa atcaatttag ctagttcttt gtcacttttt cgaatttctt
     5881 cgataacttg acttaacgaa tgcttacctg gcatggtgta aactacatcg atggcgtctt
     5941 ggatcaccgt tttcttgtgg ctcgctgttt ctcgattggt atctacttct cctaacattc
     6001 ctaacacatt tttagcggtt tgaattgcct cttcttttgg taaaaacaat agaggatcaa
     6061 gcatccctcg acagctttca tctgaagata acgaaataaa attaattcgt tcatacatag
     6121 ccacaaactc tggtatgtgt ccgaattttt caagtgcctt taaaaagaac ttttgggttt
     6181 catttttcgg atcaatataa agaatttttt gacctaaaaa catcagccat aaaaaaatat
     6241 attttacaag gaccgatttt cctttacctg gtggaccaat aatcaacgta tttccattgg
     6301 tatgttccgc gccttcaata cttttctttg ttaaattggg ataataccat actagtttac
     6361 tagatagcgc taacgcttct ttcgcagatt taaacttttt tgcaataaca atccgtccta
     6421 aaggaaatcc tttcttattt cctacttctt tctttaagtc caagccataa tctgccacaa
     6481 aacctgtcgt agcgacctgc tcgtaactac gaaaacgttg cgtacttcca attaagcatt
     6541 gattaaacaa cgttagctga tccgctaccg atcgataaaa tttgaaatct gtagactcaa
     6601 tcgcaaattc aatatctttg actcgttctt ccaattcttc ttttgaagaa gcagatacaa
     6661 ccagcgtgac ggtagaataa cacaatcgtt tttctttgcg cttcagctta tcttttacat
     6721 ccattaaccg ttctgtacca aaatttaata catcgtctga atctaaaatg ccgtcaacgg
     6781 tctcatattc tttttcttgt tcataaatcc gtccagccat tttattaatt tttcgttcat
     6841 cagattttga atgctcaaat cgaagtcgaa tatgcgtatc aattggaaaa gataagctat
     6901 cctgaagatt ttgaataaag ctactcccat acatggctaa aggagcgtcc gtttgactta
     6961 aaaacgtcga ataatgcgtt tgattcaact gttcaatcgc aatgtatcct gcttcattcg
     7021 aaatgacgcc ctcgcaaatt tccattggcg taagctgtct ttcaggcaat ttttcttctg
     7081 atcgatggaa aaaataataa taaatttgtc caatctcttg ggcagataag cgctctatcg
     7141 gcttataatt ggacaattcc gtatagagct ccttttctaa tgtttcatac gttggtaaca
     7201 cactacttat aggaactcgt tgtcccgtaa tacgatagat aaacttttta aaaacatgct
     7261 taagcaattg aaaatattcg atcggatcag acactttcat ttctgtagtg aaacgcacaa
     7321 acagatacgt tcgatactcg tttaattgca cttcgtcttt taaaatcttt cctgcctgtt
     7381 caaagtacac tttcccaaga tctgcgcatt ctccctttac caaatctcga atggtttctt
     7441 caatgtgcgt atctaggtta aattgacgtg gaatattgat aaaatgatag tcatattcat
     7501 catgtccaag tatgccttcg ccatcttgaa gatagttctt gaaaaaatca atgtcattta
     7561 aaggaaatac ttgggaagaa agttgatagc ctacccacac atcatcattt ttggtcaaaa
     7621 caaaatttcg gataatggcg tctatcggaa attctaatcg aggaagcttc gtttttttct
     7681 ttttgaactg ccaaatcccg ataataaaga cgaccaaaag aaacaaaaac gccatttact
     7741 cgactcactc cttccatttg tgtagggaat acgtcacttt ttcatggaaa aacggcacat
     7801 acatttcatg ttcaaagcgt tgtttccgtt tctgataata ggacgttcgc ccaattaaat
     7861 actcgaaaaa gtgtttattg tcccatttca ttgaaaacaa tgtccaaacg agaacaccaa
     7921 tcccaaaaat cataatcaac caggactttc gaaacagtgt ctgcaagaaa ctaatttgat
     7981 tcacatacgc aatcaaaaat aggatcaaaa gaatgaagag aaatacccca cctatgacta
     8041 attgttccat gcgaatgcca ttagaaatcc agggaatttt ttgtccttta atcccgtaaa
     8101 ttttgtgagg gtgcttaaac tcctttgtat agttgtattc catgttcact ctacctcctt
     8161 ttacaaaaaa agaagcgacc agaagttcta atcttctgat cgcttttgca gtttgtcttt
     8221 ttcttatgat aattgtatcc agggcacaat catttgcgtt acccccacgt tctcttcact
     8281 atccttaata aagataatgt tctttagcca ttcaccaata tcaacggcat tcgataacaa
     8341 taagacgagc attatcaaaa taaagacgcc aataatggca aacgctgccc gcgcaaagcc
     8401 ttgggtcgca taagcaccaa ccattagaac aatcgcaacg attgccaata gccaaatgac
     8461 ttgtccagat acatcatcaa ataacgattt aagatcaaaa agtttcattt ccattctcct
     8521 ctaagttcat tttattcatt taacgtagta acaaaataac tatcgccact tgtttttact
     8581 tccattgaaa agtttgattg gaactcgttt cctttctcaa aatagaaacg atacttccct
     8641 tgcaaaaagt agtttccttc tttatttttt actgcatgat ccagttgtac attgatcaat
     8701 tcgccgcctt ctaacccttt ttcgttagaa atcaatgcca atttttcatc atttttgaca
     8761 tataactcaa aaaaccgttt cacaaatgct tccagcttga tttcttcgct ttcagtgacc
     8821 gcttgcccag aagaataaaa tcgattttcc tgatagttca cagaattggt attcttactc
     8881 tcagaaagat tcaaaaattg aacgccatca agtactttta aaacattgtt ttgataactg
     8941 actggaaaag aaagttgact gcttttccaa tgttctcctt ctgtaaaagt aacctcataa
     9001 aataggcgat acattgcttg tgtcaaatcg atttcttcta gtttaataaa gcgaacatct
     9061 ttcgctactc gattggtagc cgtcgttaca aagcctagat cagacgcttc caatccttgt
     9121 gctaaataat gattgacgga agcctcccat tcgtttcgtt tttctggcga ataagtaaat
     9181 aacctttcaa taaaatgttg tccctcgtac ttcaattgtt ccgtagaaga caaggtttgg
     9241 ttgacttcag ttaacaatgt ttctggattg acttgttttt tcactgctaa acgcgttaat
     9301 gtatccactc gtccaaagtt taggaggagc acgacaccca ttaacagaaa caaacaaaaa
     9361 aaagcgcctg aaaatacact catggcactt ttttgcgaaa attggatcat tcgatctgac
     9421 ggttctgttt tctttttttg gaacaaaaaa cgtttctttt gtttcttttc cttctgttgt
     9481 ttcggtggct tagtttggaa cgcttgtgga ttaaattttg cattactcat gagaaatgcc
     9541 ataccgttct aacaataatt tcggtatttt gtccacaatc actacttcgt ggacaaccac
     9601 acttgccccc tc
//
LOCUS       DACDKM010000103         9428 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20834_2016
            SAMN12640370-rid9891663.denovo.103, whole genome shotgun sequence.
ACCESSION   DACDKM010000103 DACDKM010000000
VERSION     DACDKM010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640370
            Sequence Read Archive: SRR10040891
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9428)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9428)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742835.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742835.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:04:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,012
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 29 of 75
            Pseudo Genes (incomplete)         :: 33 of 75
            Pseudo Genes (internal stop)      :: 19 of 75
            Pseudo Genes (multiple problems)  :: 5 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9428
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640370-rid9891663.denovo.103"
                     /isolate="E20834_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..152
                     /locus_tag="IV334_002293"
     CDS             <1..152
                     /locus_tag="IV334_002293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP8944344.1"
                     /translation="NFAKNQGYNTILLGAQSTAETFYEKLGYTAYGDPFEDAGMPHIY
                     MKKTL"
     gene            complement(230..763)
                     /locus_tag="IV334_002294"
     CDS             complement(230..763)
                     /locus_tag="IV334_002294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF402 domain-containing protein"
                     /protein_id="HAP8944345.1"
                     /translation="MGVPKEGEFVTIQSYKHDGKLHRTWRDTMVLKTSEHSMIGVNDH
                     TLVTESDGRRWVTREPAIVYFHKKYWFNVIAMIREKGVSYYCNLASPFLLDDEALKYI
                     DYDLDIKVFPDGEKRLLDVDEYEMHSKMMNYPNDIDFILKENVKILVDWINNGDGPFS
                     EGYIDIWYNRYKQLSRK"
     gene            complement(857..2035)
                     /gene="mutY"
                     /locus_tag="IV334_002295"
     CDS             complement(857..2035)
                     /gene="mutY"
                     /locus_tag="IV334_002295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HAP8944346.1"
                     /translation="MNKEWEKWTDKETKEFQDQFIQWYEQEKRNLPWRYNRDPYRIWI
                     SEIMLQQTRVDTVIDYFYRFMEWFPTIEELANAPEEKLLKAWEGLGYYSRARNIQAAA
                     KQIMSEFDGEMPQTPEEISSLKGIGPYTTGAIASIAFGLPEPAVDGNVMRVVSRLFCI
                     EADIAKASSRKIFDEAMRKIIDEKHPGEFNQAMMDLGSAICTPTSPKCETCPIQAFCL
                     ANKRGIQTSFPVKTKKAKPKDVYYISAALQNHSGAYYFEERDSQKLLANMWTFPMMEV
                     TQEEYERLKKEWETKPEIDLFDDLVAEEEQNLPFEKQELFVWQTRHLGEVTHIFSHLK
                     WHVLLFYGRATEGAEQEFTENKTSKWLKPDAFDSVVFPKVQMKLVDQLEKNRNNRNPF
                     "
     gene            complement(2028..2825)
                     /gene="recX"
                     /locus_tag="IV334_002296"
     CDS             complement(2028..2825)
                     /gene="recX"
                     /locus_tag="IV334_002296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HAP8944347.1"
                     /translation="MLTVTRISKGKGPFYRVDLSEGEALQVSEDVLVRYRLLKGRELD
                     EETIQEIKKSSGADFGFHLAMNYLSYQLRSEKEIRTYLKEKEILQEDRNKIVARLKEL
                     DLVDDLVYGESYVRTNMRLSDKGPKKLAQQMQQKGLKPEIIEQALSQYTFEDQVQNAH
                     QTAEKAFGKNHGKSQKELLRKIQQTLMTKGFSQEVIQQAIADLPQEADQEIEYDYLVK
                     QGDKLWRRNLRFEPKKRNLKVKQSLYQKGFDLDMIQRFITEKEEESE"
     gene            2932..4314
                     /gene="rlmD"
                     /locus_tag="IV334_002297"
     CDS             2932..4314
                     /gene="rlmD"
                     /locus_tag="IV334_002297"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HAP8944348.1"
                     /translation="MAETIVKTGQTVPLKIKRLGINGEGIGYFKRLIIFVPYALPKEE
                     VLVKITKATPRYAEGELIKVKKTSKDRVVAPCPVYYECGGCQLQHLAYQAQLDFKKDL
                     LLQALEKFKPAGFRSYQLLPTIGMDEPWHYRNKAQFQLRKNKQTQKIEAGLYEANSHQ
                     LVPITDCLVQEPRTQKVMNTVVQLLNKFDAPIYDERTNSGIFRTIMVRIGVKTGELQV
                     VFITRSKKFPQKNAMIREINERLPEVVSIMQNVQAQKTSLVMGDETIHLWGKEAIEEQ
                     INEVTFDLSPRAFFQLNPQQTELLYGEGIKALDVQPNETIVDAYCGVGTIGLSVAKQA
                     KEVRGMDTVPQAIADAKKNAERLGYHNTRYEVGTAEQVLPKWLNEGFQPDGIIVDPPR
                     TGLDNALIQSLLKYPPKKLVYISCNVSTLARDLVNLSKKFKIEYLQSVDMFPQTARCE
                     VVVKMTRLAD"
     gene            complement(4488..5066)
                     /locus_tag="IV334_002298"
     CDS             complement(4488..5066)
                     /locus_tag="IV334_002298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chitin-binding protein"
                     /protein_id="HAP8944349.1"
                     /translation="MKKISTLLFIGLVSLGLFGFSIPGFAHGYITSPGSRAYLGTSAA
                     GNLNQNVGRAQWEPQSIEATKNTFIDGKIASAGVSGFEPLDEQTSNRWHKNMVNPGTL
                     NITWNLTAQHRTSTWDYYITKPTWNPNQPLKFSDFELITKIDDKATVPPKTVNQTITL
                     PQDRKGYNVILAVWNISDTTNAFYQVIDVNIQ"
     gene            5388..5858
                     /locus_tag="IV334_002299"
     CDS             5388..5858
                     /locus_tag="IV334_002299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan-rich sensory protein"
                     /protein_id="HAP8944350.1"
                     /translation="MKMIKDIRFWVCVIGIVILGFLSGLLSGNPGKYYYSLQLPPFAP
                     PSWIFGPMWTLLYILMGISLYLLLNHNNKKQRNNLVGLFVIQFIFNFIWSALFFNLRN
                     IFIAAIDITLLVIFLSVLMYQLWLHHRLTMWLMIPYYLWVLFATLLNYSIYFLN"
     gene            complement(5966..7192)
                     /locus_tag="IV334_002300"
     CDS             complement(5966..7192)
                     /locus_tag="IV334_002300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LytR family transcriptional regulator"
                     /protein_id="HAP8944351.1"
                     /translation="MKRWQKVLLTLLGVFVLLIAGLSAYGIRFMGEASQTVNKISKNS
                     NRISSKRSERVSIDDKEPFSVLLLGLDTGGLGRTEQGRSDTMMVVTVNPKQKKSTIIS
                     LDRDIYTNIVGYGTVDKLNHAYAFGGVEMAMDSIEQLLDIPIDHYVTINLDGMEDLIN
                     AVGGIKVNNKIDFTLDGVHVPVGEQTLNGEKGLAYSRMRHEDPEGDIGRQRRQREVVT
                     KIVNKVLSLDGVSNYRKILKAVENNVTTDLDWDDMLDVATNYTPAFETIKQDQLQGEG
                     QMINDIYYQILGKNDLLSIQNELKKQLNIKTSDTLPNLKNENASMMFFDDSEDGDSTN
                     GDGSNSEGYTDYSSYSENTYDQYQDPNTYYQQTEDTSQYYTPSQDTQSYDPYAGQEQN
                     GYGYDQNNEYGTTSGY"
     gene            complement(7946..8146)
                     /locus_tag="IV334_002301"
     CDS             complement(7946..8146)
                     /locus_tag="IV334_002301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HAP8944352.1"
                     /translation="MEHGTVKWFNNEKGFGFITVDGGDDVFVHFSAIQGDGFKSLEEG
                     QEVEFTIVEGARGPQAAEVTKL"
     gene            complement(8280..8888)
                     /locus_tag="IV334_002302"
     CDS             complement(8280..8888)
                     /locus_tag="IV334_002302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HAP8944353.1"
                     /translation="MTYTLPDLPYAYDALEPYIDEETMHLHHDKHHNTYVTNLNSAIE
                     KYPELGEKTIEELLSDMDAIPTDIKTAVRNNGGGHANHSFFWEIMAPNAGGEPTGEIK
                     EAINEAFGDFSSFKEEFKKAAAGRFGSGWAWLVMENGKLAITSTANQDSPLMEGKTPI
                     LGLDVWEHAYYLKYKNVRPDYIAAFWNVINWDEVNKRFEAAK"
     gene            complement(8973..>9428)
                     /locus_tag="IV334_002303"
     CDS             complement(8973..>9428)
                     /locus_tag="IV334_002303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HAP8944354.1"
                     /translation="QLKGYLEAKGYWEILVYLFFGGLATIVNFVSFALARQYFDLSMA
                     LSNSISWFCSVLFAFVTNKLWVFHSKSPNFTHALIECGKFFFYRILSYGLDMGAMVLL
                     INVMNSNEYVAKIITQIIVILANYIFSKLFIFKETEVFEEEVGQSEKDD"
BASE COUNT         2797 a         1890 c         1560 g         3181 t
ORIGIN      
        1 aaaactttgc taagaatcaa ggctataata cgattttatt aggggcacag tccacagctg
       61 aaacttttta cgaaaaacta ggatatacag cttacggcga tccttttgaa gatgctggca
      121 tgcctcatat ttatatgaaa aagactttat aaaaatttat ttcgaaaaaa gcaaaaaaca
      181 gcaagctttt gagaaaaatc tctcactggc ttactgttct tttttgtgtt tacttacgcg
      241 acaattgctt gtaacggtta taccaaatat caatatagcc ttccgagaac gggccatctc
      301 cattattgat ccagtcaacc agaatcttca cattttcttt taggatgaag tcaatatcgt
      361 tcgggtaatt catcattttg ctgtgcatct catattcatc gacatccaat agtcgtttct
      421 cgccgtcagg aaataccttg atatccaagt catagtcgat atatttcaag gcctcatcgt
      481 ccagcaaaaa aggtgaagct agattacaat aataggaaac ccccttttct ctgatcattg
      541 ctattacatt gaaccagtat tttttatgaa aataaacaat cgctggttcg cgcgttaccc
      601 atcggcgtcc atcagattca gtgactaatg tgtgatcatt cacgccgatc atagagtgtt
      661 cgcttgtttt taataccatg gtatctcgcc acgttcgatg caatttgccg tcgtgcttgt
      721 aactttggat cgtcacaaac tctccttctt ttggaactcc catagcaacc cttccttaag
      781 cagcattttg tgctttgtgt ttgggtgttc attacaccga catcataatt attatatcaa
      841 taaaaatgag ctttgcctag aaaggattcc tattgtttcg atttttctct aactgatcta
      901 ccagtttcat ttggacctta ggaaaaacga ccgaatcaaa tgcatcaggc ttcaaccact
      961 tcgaagtttt gttctcggta aattcttgtt ctgctccctc ggttgcccgt ccgtaaaaaa
     1021 ggagaacatg ccatttcaga tgactaaaaa tatgagtaac ttctcctaaa tgtcgtgtct
     1081 gccaaacaaa gagttcctgc ttttcaaatg ggagattttg ctcttcttct gctactaggt
     1141 catcaaacag atcgatttct ggctttgttt cccattcttt tttcaatcgc tcatactcct
     1201 cttgtgtcac ttccatcatt ggaaatgtcc acatattcgc cagcaatttt tggctatctc
     1261 tttcttcaaa ataatacgct ccagaatgat tttgaagagc tgcactgata taataaacgt
     1321 cttttggctt tgcttttttt gtttttacag gaaaactcgt ttggattcct cgtttgttag
     1381 ctaaacaaaa ggcttggatc ggacaagtct cacactttgg agaagttggt gtacagatag
     1441 ctgaacctag atccatcatt gcttggttga attcacctgg atgcttttca tcaatgattt
     1501 tccgcatcgc ttcatcaaag attttcctgc tagaagcttt tgcgatgtct gcttcaatgc
     1561 aaaacaatct gctgactact cgcatcacgt tgccgtcaac tgcaggttct ggaagtccaa
     1621 atgcgatact tgcaatcgcg cctgtcgtat atggtcctat tcccttcaat gagctaattt
     1681 cttcaggcgt ttgcggcatt tccccatcaa attctgacat gatctgttta gcagcagctt
     1741 ggatgttccg ggctcttgag taataaccaa ggccctccca tgcttttaaa agcttttctt
     1801 ctggagcatt tgctagctct tcaattgtag gaaaccattc catgaaacga taaaaataat
     1861 cgatcactgt gtctactctg gtttgctgaa gcatgatctc agagatccag atacgataag
     1921 gatcacgatt atagcgccaa ggcaggtttc ttttttcttg ttcataccat tgaatgaact
     1981 gatcttgaaa ttcttttgtt tctttatctg tccatttttc ccattcttta ttcactttcc
     2041 tcttcctttt ccgtaatgaa tcgctggatc atatccaaat caaatccttt ttgatataag
     2101 ctttgcttta cttttagatt tcttttttta ggttcaaacc ttaaatttct tcgccatagt
     2161 ttatctcctt gtttgactaa ataatcatat tctatttctt gatctgcttc ttgcggaaga
     2221 tcagctatcg cttgttggat cacttcttga gaaaagcctt tcgtcatcaa tgtttgttgg
     2281 atttttctca ataattcttt ttggcttttt ccgtgatttt ttccaaacgc tttttcggct
     2341 gtctgatgtg cattttgaac ttggtcttca aatgtatatt ggctgagcgc ttgttcaatg
     2401 atttcaggtt tcaaaccttt ttgctgcatc tgctgcgcta atttctttgg tcctttgtca
     2461 cttaagcgca tattcgtgcg cacataactt tccccataga ccaaatcatc gacaaggtcc
     2521 aattctttta agcgggctac gattttattt cgatcttctt gaaggatttc cttttctttt
     2581 aagtatgttc ggatttcttt ttccgatcgc aactgataac ttaagtaatt cattgctagg
     2641 tgaaaaccga aatcagctcc gctactcttc ttgatctctt ggatagtttc ttcgtctaat
     2701 tcccgtcctt ttaataatcg atagcgtact aatacatctt cagatacttg taaagcttct
     2761 ccttcagata aatctacgcg atagaaaggg cctttgcctt tactgattct cgtaacggtt
     2821 aacataaaca tcctcctcga acatatattc tctatcatac cacagaaaat ccccgtaatc
     2881 aggtattata tgttagaata aaccacagaa aaaagaaaaa gggagatact tatggcagaa
     2941 acaattgtca aaactggaca aacggttcct ttgaaaatca agcgtttagg cataaatggt
     3001 gaaggaatcg gttatttcaa acggttgatc atatttgttc catatgcact accaaaagaa
     3061 gaagtactag tgaaaatcac gaaagcaact cctcgctatg cagaaggaga attaataaaa
     3121 gtcaaaaaaa caagcaaaga tcgcgtagtg gcaccatgtc ctgtttacta tgaatgcggt
     3181 ggctgtcaat tacaacactt agcttatcaa gctcaattgg attttaaaaa ggatctgctt
     3241 ttgcaagcat tggaaaaatt caaaccagca ggctttcgat cttatcaact tttgccgacg
     3301 atcgggatgg atgagccttg gcattataga aacaaagcac aatttcagtt acgaaaaaat
     3361 aagcagactc aaaaaattga agctggttta tacgaagcaa actctcatca gttagtccct
     3421 ataacagact gcttggttca agaacctcga acacaaaaag taatgaatac tgttgttcaa
     3481 ctattgaata aatttgatgc acccatctat gatgaacgga caaacagtgg aatcttccgc
     3541 acaatcatgg ttagaatcgg tgttaaaact ggggagttac aagttgtttt tattactcgc
     3601 agtaaaaaat tcccgcaaaa aaacgcaatg atccgtgaga tcaatgaacg actgcccgaa
     3661 gttgtttcaa tcatgcaaaa tgtccaggca caaaaaacgt ctctcgtgat gggggatgaa
     3721 acgatccact tatggggaaa agaagccatt gaagaacaaa tcaatgaagt gactttcgat
     3781 ctttctcctc gtgccttctt tcaactcaat cctcagcaga ccgaattgct ttatggcgaa
     3841 ggaatcaaag cattagatgt ccagcctaat gaaacgatcg tggatgctta ttgcggagtt
     3901 gggacgatcg gcttaagtgt agcaaaacaa gccaaagaag tacggggaat ggataccgtt
     3961 cctcaagcaa tcgctgatgc aaaaaaaaat gcggaaagat taggatatca caacactcga
     4021 tatgaagtcg ggacagcaga gcaagttctg cctaaatggt tgaatgaagg ttttcagcct
     4081 gatggtatca tcgtagatcc accacgtacg ggtttggata acgcattgat ccaatcatta
     4141 ttgaaatacc ctccaaaaaa acttgtttat atctcctgta atgtttctac acttgccaga
     4201 gatctggtta atttaagtaa aaagttcaag attgaatatt tacagtccgt cgacatgttt
     4261 cctcagactg cacgttgtga agttgtcgtg aagatgacga gactcgctga ctaaatgaaa
     4321 gaggttgtga aaaagtctgc cctgtatcaa gtaataagaa cagccaaaca aaaatagctc
     4381 ttcatgtttt atgaatactg tacttgtgac cgaagagtta acaggaggac actatttatt
     4441 taaaaaaagg gttgtgacat agtttttgtc acagcccttt ttgattatta ttggatattc
     4501 acgtctatca cttgataaaa tgcatttgtc gtatcgctga tattccatac tgctaaaatc
     4561 acattatatc cttttcgatc ctgcggaagt gtgatcgttt ggttgactgt ttttggtgga
     4621 acagttgctt tgtcgtctat ttttgtgatc aattcaaaat cactaaattt caaaggttga
     4681 ttaggattcc aagttggttt ggtaatgtag taatcccaag tacttgtccg atgttgtgct
     4741 gttaaattcc aagtaatatt taatgttcct ggattaacca tgtttttatg ccatctgtta
     4801 gatgtctgtt catctaaagg ctcaaagccg ctgactcctg cactagcaat ttttccatca
     4861 atgaatgtat ttttggtagc ttcgatactt tgcggttccc attgtgctct tccgacattt
     4921 tgatttaagt ttcctgctgc tgatgttcct aggtatgcac ggcttcctgg agaagtaata
     4981 taaccatgcg caaaacctgg aatgctaaaa ccgaacaacc ctaaactaac taacccaata
     5041 aacaataacg tactaatctt tttcatgtag aaacctcctt catttgataa ccacattgta
     5101 aacgctttat tgtattaaca caaacattat gcttttataa ttttatgttg aagtaaaaaa
     5161 ggtgaaaaaa gtaagatgca gattataaca gaagtagacg agaaaagtac aaatagaaaa
     5221 catgagaatc aaatcatatg atctttttca aaaacatcat tcatataaac aaatgttcaa
     5281 ctttttaata acaacaatta tcaatttttt tcttacttca caaactaaat acaggtcttg
     5341 ctgtgagcct tcatggtaaa ctaatattat agaaaggagt tagtaaaatg aagatgataa
     5401 aagatattcg tttttgggtt tgtgttatag gaattgtcat attaggcttt ttatcagggt
     5461 tgcttagcgg aaatcccgga aaatattact attctttgca acttccacca tttgcaccac
     5521 ctagctggat atttgggccg atgtggacct tactgtatat attgatgggt atttctcttt
     5581 atttgttact taatcacaac aacaaaaaac agcgaaacaa tcttgtcggt ctttttgtga
     5641 ttcagttcat ttttaatttt atatggtctg ctctattttt taatttaaga aatattttca
     5701 ttgcagcaat cgatattaca cttttagtta ttttcttgtc agtgcttatg tatcagttgt
     5761 ggctgcatca tcgcctgaca atgtggctca tgattcctta ttatttatgg gtactattcg
     5821 caactctatt gaattattca atttattttt taaactaagc gctaaaaaga gacgtcctgt
     5881 aatttaacac atagaaaaag tgccacctaa gttagatttt ttgtctaatt taggtggcac
     5941 tctttattaa ccaaactatt tttttctagt acccacttgt tgttccatac tcattattct
     6001 ggtcataacc ataaccattt tgttcctgtc cagcgtaagg atcataactt tgagtatctt
     6061 gacttggtgt atagtactga cttgtatctt ctgtttgctg ataataggta ttcggatctt
     6121 ggtactgatc atatgtgttt tctgaatagg aagaataatc ggtgtaccct tcactattcg
     6181 atccatcacc gtttgtcgaa tcgccatctt ccgaatcatc aaagaacatc attgaagcat
     6241 tctcattttt caaattaggt aatgtatcgc ttgtttttat atttaattgt ttcttcagtt
     6301 cattttggat cgaaagaaga tcattttttc ctaaaatttg atagtagata tcattgatca
     6361 tttgtccttc gccttgcaac tggtcttgtt tgattgtttc aaacgccggg gtgtagttcg
     6421 tcgctacatc aagcatgtca tcccaatcta agtccgtcgt cacattgttc tcaactgctt
     6481 ttaaaatttt tctataattg cttacgccat ctaaacttaa aactttattg acgattttcg
     6541 tcactacttc ccgctgacga cgttgtcgac cgatatcgcc ttctggatct tcgtgtcgca
     6601 ttcgagaata ggctaaacct ttttctccat tcaatgtttg ttcacctact ggtacatgga
     6661 caccatccaa agtaaagtca attttattat tcactttgat gccaccgacc gcattgatca
     6721 aatcttccat gccatccaaa ttgatcgtta cataatgatc aataggaata tcgagcagtt
     6781 gctcaattga atccatggcc atttcgacac caccaaatgc gtaggcatga tttaatttat
     6841 ctaccgttcc ataacctacg atattcgtat aaatatctcg gtccaaactg atgatcgttg
     6901 attttttttg tttcggatta actgttacta ccatcatcgt atcagaacgt ccttgttccg
     6961 ttcttcctaa tccaccggta tctaaaccta atagcaaaac cgaaaaaggt tctttgtcat
     7021 caatgcttac tctctcagaa cgtttggagg atattcgatt agagttttta gaaattttgt
     7081 ttactgtttg gctcgcttct cccataaatc tgattccgta tgctgataat ccggcaatca
     7141 gcaaaacgaa tacccctaac aaggttaata gaactttttg ccatcgcttc ataatacacc
     7201 tctgtttcac attttctaaa atattgtctc atataactgt tcaatcagca aattcacttt
     7261 ttaatcataa gaaaacttta aaaaacattt ttgtgtaacg attattattt tttaaaattt
     7321 ctcaaaattc ttaactatca taccataccg tgaaaaagat cgaaagaccg aatacacgca
     7381 gttcgcaatc gttttatctt tttttatgta ttttttccag aaaaagcgaa caacaaggta
     7441 ttttccaaat aatcgtgcag taaaatgatc gcacatacct ttcccatctt tttaacagtc
     7501 tttgcctacc gttcagatta tcattcgatt tcacgacaat ttcttcctat gactggtcct
     7561 tttgttaaac ccatcttcta ttcgttttat ttccagctca ctgcatggct cttaaaatga
     7621 ctcgaactag tgattgcatt accatcatct tttaatggat agatatgctc ctcttcaaag
     7681 taaaacgatt ttcttttaaa gtaatagaat tgacatggta atgagtatgt atactagtgg
     7741 tttcacaaat ggtcttttat tcaacatcca aaaactgaaa tttctttaaa atccattact
     7801 aacgaagaaa atgttccttg cttgataatt agttaggaca tgtcaagtga acaaatgcat
     7861 ttacttagcc aaaaaatcta aaaacttcat aaatagacaa aaagacacga ctctctaaga
     7921 agtcgtgtct ttattcaaca aattattata atttagtcac ttcagcagct tgaggtccgc
     7981 gtgcaccttc aacaatcgtg aattctactt cttgaccttc ttctaggctc ttgaagccgt
     8041 ctccttgaat tgctgagaaa tgaacaaata cgtcatctcc gccgtctact gtaataaacc
     8101 caaatccttt ttcattgttg aaccatttaa ccgttccgtg ttccataaat gtatatcctc
     8161 caaaaaagtt cttgcaagta accttgcaag aacattatac ccaatttaga aaacgctagc
     8221 aagctttcgc tcttttattt tccaaattat ttttaagact gatatgacgg aggaaatggt
     8281 tattttgctg cttcgaaacg tttgtttact tcatcccaat taataacatt ccagaatgct
     8341 gcaatataat ctggtcggac atttttatat ttcaaataat aagcatgttc ccaaacgtcc
     8401 aaacctagaa ttggtgtctt tccttccatc aatggagaat cttgatttgc agtagaggta
     8461 atagctaatt ttccattttc cattacaagc caagcccatc cagaaccaaa tcgtccagcg
     8521 gctgcttttt tgaattcttc tttaaaagaa gaaaaatcac caaaagcttc attaatcgct
     8581 tcttttattt ctcctgtagg ttcgccacca gcatttggtg ccatgatttc ccagaaaaat
     8641 gaatggttag catgtccgcc accattgtta cgtacagctg tcttgatatc tgttggaata
     8701 gcgtccatat cagataataa ttcttctatt gttttttcgc ctaattctgg gtatttctca
     8761 attgctgaat ttaaattcgt cacataagta ttgtgatgct tatcatgatg cagatgcatc
     8821 gtttcttcgt caatataagg ttccaatgcg tcatatgcat aaggtagatc tggtaatgtg
     8881 taagtcataa aaaaattcct cctcaatgtt ttcttaataa tgtctacatt acaaagagta
     8941 acaaagagaa ggaaattctt caattgttat gcctagtcgt ctttttcact ttgacctact
     9001 tcttcttcaa aaacctcagt ttctttaaaa ataaacaact tgctgaaaat atagttcgca
     9061 aggataacaa taatttgagt gatgattttc gccacatact cattgctgtt cataacattg
     9121 atcaacaata ccattgcccc catgtccaat ccgtaagaaa ggatacggta gaagaagaac
     9181 ttaccgcatt caataagtgc atgtgtaaaa ttcggtgatt ttgaatggaa tacccaaagt
     9241 ttgttcgtta caaaagcaaa caatacagaa caaaaccacg aaatagaatt ggataaggcc
     9301 atacttaaat caaaatattg gcgtgcgaga gcaaaactaa caaaatttac aatcgtagcc
     9361 aaaccaccaa aaaagagata gactagtatc tcccaatatc cttttgcttc taaatatcct
     9421 tttaactg
//
LOCUS       DACDKM010000104         9421 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20834_2016
            SAMN12640370-rid9891663.denovo.104, whole genome shotgun sequence.
ACCESSION   DACDKM010000104 DACDKM010000000
VERSION     DACDKM010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640370
            Sequence Read Archive: SRR10040891
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9421)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9421)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742835.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742835.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:04:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,012
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 29 of 75
            Pseudo Genes (incomplete)         :: 33 of 75
            Pseudo Genes (internal stop)      :: 19 of 75
            Pseudo Genes (multiple problems)  :: 5 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9421
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640370-rid9891663.denovo.104"
                     /isolate="E20834_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(<1..432)
                     /locus_tag="IV334_002304"
     CDS             complement(<1..432)
                     /locus_tag="IV334_002304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAP8944355.1"
                     /translation="MDNHTRKLLGLTDKHLFFDEEWLIEKEYKGVQAQFIKGKLDDSP
                     SYCEHCRSVRIIRNGSYTTRTQILKVKEKLTILELKRTRFLCHDCGRTFSAKTDLVDE
                     HHQLTKELKQAILMELYENQSRKLIAKKYFVSDGTVTRILRE"
     gene            633..1847
                     /locus_tag="IV334_002305"
                     /pseudo
     CDS             633..1847
                     /locus_tag="IV334_002305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611665.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
     gene            complement(2026..2361)
                     /locus_tag="IV334_002306"
     CDS             complement(2026..2361)
                     /locus_tag="IV334_002306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010756787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HAP8944356.1"
                     /translation="MKMNTNDIATVYVAFTAAEGGKRRPVYILKNGLDTISFLSITSK
                     YHTKSDKIKLKYVEIRDWKQAGLKKESWIDVGTLNELPKNNKKVKWKKIGELSPSDLQ
                     RLEERMEEL"
     gene            complement(2345..2617)
                     /locus_tag="IV334_002307"
     CDS             complement(2345..2617)
                     /locus_tag="IV334_002307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelB/DinJ family
                     antitoxin"
                     /protein_id="HAP8944357.1"
                     /translation="MEKMAKLQISMDEKLKNEAESIIDELGMSPKTAVTVFYKQIVEQ
                     GKIPFSIELSEKSKRIHNIQRLAEKLPVQKLDTDEKIEEWFNEDEY"
     gene            complement(3068..3871)
                     /locus_tag="IV334_002308"
     CDS             complement(3068..3871)
                     /locus_tag="IV334_002308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HAP8944358.1"
                     /translation="MRFLHLSDIHFRENYQTNLFTAELFPTIKNPQLALEKLLQELSL
                     TVYDFILLTGDLVHEGGISDYQVLRKMLDQYFPTTPYYFCRGNHDRRSVFFEGMSVES
                     NETGEYIHCTNQNGLRIISLDSAQDTQHEGKISIGQMIQLQEWLGTPAEKGTILLVHH
                     PLSWEDPSIVTEVPEGFAELIAESDILGIFVGHIHQGSSAEFAGKKQFMAESLSFGVD
                     EYPDYSLFTNRTGFTSGSLIDGKIFTYQHYLTPHQIEIGRMKKPITGNH"
     gene            complement(3849..4973)
                     /locus_tag="IV334_002309"
     CDS             complement(3849..4973)
                     /locus_tag="IV334_002309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP8944359.1"
                     /translation="MGETLELRNIGKKYGEHTILKEIDLAVAAGERIVLLGPSGSGKS
                     TLLRMIAGLEKITSGELFLGTQLANKLPCGEREIAMVFQNYALYPHMTVKENIIFALR
                     ANKIKTADIQHRLEEALKMLGLTPYQDRLPKDLSGGQRQRVALARAVVKRSNYFLLDE
                     PLSNLDVRLRLDARKELVKIHQRYKQTFVYVTHDQIEAMTLADRIVLLNEGRIQMIDT
                     PKNVYTKPKNTFTANFIGSPGMNIMPVTYDQGTLKVASQFLTLNPAWQKQLLQVSGSL
                     YLGIRPEHVTFSKETSDLHGQVKYRELLGQSYAYTVKVDGIEMIVLNEKPTWQIGDAV
                     NLRIEPERMHFFDYQTQENLGYPDYFIEERGNSHALFASL"
     gene            complement(4989..5804)
                     /locus_tag="IV334_002310"
     CDS             complement(4989..5804)
                     /locus_tag="IV334_002310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006739361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAP8944360.1"
                     /translation="MKIKRWLPHLLFMFIIIIIIYPLLFIMANSFKPLKDAYQTITSL
                     IPQPFTLENFCYLFENLPIIKITRNTFFIATIITVVKLGVAFFAAYSFTYCRIKGKKI
                     WYFIIIGTMFIPFTVAMIPNYLLIAKLNLIDTTWGVIIPQFADAMGIFLLTQTMREIP
                     VSLLDVAALDNIKQTTVMSKIVFPLTRHAITSTGIWFFITSWNEYVWPVLILRTVDNY
                     TLPLAMQTYISSEGGTNFTVAMAISLITMFVPLVLYAIFQKYIIGTFVSAGIK"
     gene            complement(5794..6675)
                     /locus_tag="IV334_002311"
     CDS             complement(5794..6675)
                     /locus_tag="IV334_002311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006739050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP8944361.1"
                     /translation="MSEMKRYVGYLYLAPAIILLGVFVFYPLIYTIYLSFFDWNMVAP
                     TKEFVGFANYVKVLTDPVFHKVLKNTLSYTVLFVLLSCVFPYITSFLLGIVIEKGQNL
                     YKPLFFIPAVISLVVAGMVFTWILNPVSGPIAVLLGNFGLRLPMWMNLQGWVIAVIAG
                     ITAWKVFGYNFIVIFAAIVGIDQEMIEAAKLDRVPIWRIFFTIVLPMSSSTGLYLLVM
                     TIVQGLQYVFTPIKIITRGGPNNGSANVIYYAYQKAFEMYKVGESSAISVLTLLIFAV
                     FLGVIFKFVEKGVYYEN"
     gene            complement(6751..8040)
                     /locus_tag="IV334_002312"
     CDS             complement(6751..8040)
                     /locus_tag="IV334_002312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002581297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAP8944362.1"
                     /translation="MLKKNLVIGVVSVLSVISLAACGSGNTKTTKKSEGRVTIEYWHV
                     NADTLGGKTVNELVDKYNQSQDKVKVVAKYNPDMYKGLMQNLQASVASKEIPDVVQVG
                     WAFKDYFAENFKYTNPQSLVEEVDPDNADYFKDHFLDNIMALSEKDGKQVGIPYSISN
                     PVLYLNQDILKAAGLDENGPKTWEEVQTFSKIIKEKTGKYGVYIQEPADNWAQQAILE
                     SNGASVIKDGKATFASKDGIAAYELYQRMVKDQTALHTTWEQGVQSFIDGNVAMVYTT
                     IAQRDNIQKNSTFTAKAVESPSWEGKKVKLPAGGAMLAVTSQTDKKKEATWDFLKYLY
                     SVESLAKWTEGTGYLPPRKDVADAQNGLKAFLAENQMMIPAIDQMDKMVSWSSFPGDA
                     GLEAEQKLLDMRDKILSGNSVNETMKATQKEINELLK"
     gene            complement(8268..8912)
                     /locus_tag="IV334_002313"
                     /pseudo
     CDS             complement(8268..8912)
                     /locus_tag="IV334_002313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303552.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
BASE COUNT         2909 a         1991 c         1515 g         3006 t
ORIGIN      
        1 ttcacgcaag atccgtgtca ccgtcccatc cgagacgaag tacttcttag cgattagttt
       61 acgtgattga ttttcgtaca gttccatgag aattgcctgc ttcagttcct ttgttagttg
      121 atgatgttca tcaactaagt ccgttttcgc agaaaaggtc cttccacaat catggcagag
      181 aaaacgggta cgttttaatt ctaaaatagt taacttttct ttgactttca atatctgagt
      241 gcgtgtcgta taggaaccgt tgcggataat acgaaccgat cgacagtgtt cacaatagga
      301 aggagagtca tccaattttc ctttaataaa ctgcgcttga acgcctttat attctttttc
      361 aatcagccat tcttcatcaa aaaataaatg tttatctgtt aatccgagta attttctagt
      421 atgattatcc attgagagac tcctttggta ggatgacgtt ttggtcgact aaattttacc
      481 aaaagtgagt ctctttttga gttgaaacga agaaaaagtg ttagtagaaa atcgccttcg
      541 attttccacc aacacaaaat attatagaag caaaatcaat tctaccttag atgttcttat
      601 atcactctat cagtcctatt tgacaaagag ccaagaaaaa gacgttggtc gtcgatgctg
      661 ttctctcgcc tactcctcgt gcttgtagaa gttgtggttc tactgtggta gatggaaacg
      721 ggaaagcaat tatagtgaaa aatgggaaga aggaaacgat tgtccgtttt gaacaataca
      781 atcatatgcc tttggttacg cgcctaaaaa agcaacgcta tacctgtaaa aactgtcgta
      841 cccattggac ggctcaaagt tattttgtcc gactcagcca ttcaatcgca gatcatgttc
      901 gatataaaat tgcttcttta ctgactgaaa aagtatcttt gtcttttatt gcgaaaagtt
      961 gtcaggtatc tttgactacc gttactcgta cattgaaaga gtttaaaagc tatttaccaa
     1021 agccttctaa gaagattctc cccagagtat tgatggttga tgaatttcgt tcgcacgctt
     1081 ccatagaaga taaaatgagc tttatttgcg cagatggtga aacaggaaac ttaattgctg
     1141 tcttgcctac gcgtaaatta cctcgattaa caagctattt cttaggctgc accaatccag
     1201 aagaagtaga attcttagtc acagacatga acgccgctta cttccagctt accaaacgtg
     1261 ttctgccaaa tgcgaaagta gtgattgatc ggtttcatat tgtcaaacac atgaatcagg
     1321 cactcaatga gttgcgtatc cgtgaaatga atgagcttcg taaagcagga cagaaaagtc
     1381 aggcagaaaa actgaaaaag aactggcgct ttctgctaaa aaatcgtgca agcatcaacc
     1441 attatgagta caaaacatgg aaaagtttcc gagcaccaaa atacccattt ctcactgaag
     1501 caatgatgat tgatcgattg cttgagtttt ctgcgcctct aaaggaggcg tatccctttt
     1561 ttcatgaatt agtcgaagcc tttcgagaca aagaccctga cttatttttc tccttattgg
     1621 cagaacttcc cgaaacgtta gatgacggct ttcgggagaa gcttcaaaac cttctgacct
     1681 atgaagaagg tatcaccagc gcaatgatct atccttattc caatggaaaa attgaagcga
     1741 agaataccca cataaagaca atgaaacgag tatcctacgg atttaaatca ttcgagaaca
     1801 tgagaattag aatctttttg atcaatcaat taatcaatgt aagataacaa aaaatctgaa
     1861 ccagaatgtg gttcattctg acccggattt gattttgcac aactcatcag tccttattga
     1921 caaagagcca aaaatctgag ccagaatgag gttcattctg acccagattt gattttgtac
     1981 aacccatcag tccttattga caaagagccc ttctttttag tattattata attcttccat
     2041 gcgttcttcc agtcgctgta aatcgcttgg acttaattcc ccaatttttt tccatttgac
     2101 ctttttgttg tttttgggta attcgtttag agttcctacg tcaatccaag attctttttt
     2161 tagtcctgcc tgtttccaat cccttatttc aacgtatttc agctttattt tatcggattt
     2221 agtatgatat tttgaagtaa ttgacagaaa cgaaattgtg tccagtccat tttttagaat
     2281 atagacaggc cggcgtttac cgccctctgc agctgtgaaa gcaacgtaga cggttgctat
     2341 atcattagta ttcatcttca ttgaaccatt cttcaatttt ttcgtcagtg tctagttttt
     2401 ggacaggcaa tttttccgct aatctttgaa tattatggat tcgtttactt ttttccgaca
     2461 attcaataga aaatgggatt tttccttgct caacaatttg cttataaaaa acggtgacgg
     2521 ctgttttggg gctcatgcct agttcatcaa taatgctttc tgcttcattt ttcaattttt
     2581 cgtccataga aatttgtaac ttagccattt tctccacctc tttctgacaa acacattata
     2641 tcacggaaag agttcaaatg gaattaatac aactactttt gaactccttg aaatgcagtg
     2701 tatttatcta aatataagga aaggatatag tgctaaatgg ccttgataga tagaaaagtt
     2761 tcaaaatccg ccacagcgat aattgaagcg aacactcaac gacacacaga acaggaaaag
     2821 actcctaaaa gaaccaaatt aactaggacg tgtctgaaaa ctccacaaag atgtggtatg
     2881 ctctggtaaa aaacgaatgg cacaaccacg aagaccgata tggttcttgg aaaactttct
     2941 ccaccctttt tgtaattgga gggatagtgg tttattgata gcgattttcc aagttcttca
     3001 agtggaacca gacattgagc accacggcta aaaagtgaat gaagtcttta tttttaatca
     3061 ataattttta atgattacca gtaattggtt ttttcatgcg ccctatttct atttgatgtg
     3121 gagttaaata atgctgataa gtaaaaattt ttccgtcaat taaactgcca ctagtgaatc
     3181 ctgttctatt ggtaaaaaga gaatagtctg ggtattcatc tacaccaaat gataaagatt
     3241 cagccataaa ctgtttttta ccagcaaatt cagcactgct accttgatgg atatgtccta
     3301 caaaaatacc taaaatatca ctttcggcaa tcaattcagc aaaaccttcc ggaacttcag
     3361 ttacaataga tggatcttcc cacgacaatg gatgatgaac caataaaatc gttccctttt
     3421 cagcaggtgt acccaaccac tcttgtaatt gaatcatctg tcctatcgat atttttcctt
     3481 cgtgctgggt atcttgagca gaatccaagg aaataattcg taacccattt tgattcgtac
     3541 aatggatata ctctcccgtt tcattgcttt caacactcat tccttcaaag aaaacactgc
     3601 gacgatcatg attcccacga caaaaatagt aaggtgtggt tgggaaatat tgatccaaca
     3661 ttttccgtaa gacttgataa tcagaaatcc ccccttcatg tactaaatca cctgttaata
     3721 gaataaaatc gtacactgtt aacgagagtt cttgtaacaa tttttccaat gccaactgtg
     3781 gatttttaat tgttggaaat agttcagcag taaacaagtt agtttgatag ttttctctaa
     3841 aatggatatc agagagatgc aaaaagcgca tggctattcc ctctttcttc aataaaataa
     3901 tcaggatatc ctaaattttc ttgcgtctga taatcaaaaa aatgcattct ttcaggttct
     3961 atgcggagat tgactgcatc accaatctgc caagtaggtt tttcattaag gacaatcatt
     4021 tctataccat cgactttaac ggtgtacgca taactttgac ctaatagctc acgatacttc
     4081 acttgaccgt gtaaatcgct tgtttctttt gaaaaggtta cgtgttctgg tcttatgcct
     4141 aaatacaatg agccactaac ctgaagaagt tgtttttgcc aagctggatt caatgttaaa
     4201 aattgactgg ctacctttaa tgttccttga tcataagtaa ctggcataat attcatgcct
     4261 ggcgaaccaa taaaatttgc agtaaaagta tttttaggtt tcgtataaac atttttcggc
     4321 gtatcaatca tctgaattct accttcattc aataaaacaa tccggtcagc taacgtcatc
     4381 gcttcaattt gatcatgagt gacgtagaca aaagtttgtt tataacgttg atgaattttt
     4441 accaattctt ttcgtgcatc caatcgtaaa cgcacatcca aatttgataa cggttcatct
     4501 aataaaaaat aattactccg tttaaccacc gcacgagcca atgctacacg ctgccgttgc
     4561 cctcctgaca aatctttagg aagtcgatct tggtatggcg ttaaacctaa catcttcaaa
     4621 gcttcttcca aacggtgctg aatgtcagct gtcttaattt tattcgccct taatgcaaaa
     4681 ataatattct cctttacagt catgtgagga tataaagcat aattttgaaa aaccattgcg
     4741 atctctcgtt ctccacaagg tagcttattt gctagttgtg tacctaaaaa aagctctcca
     4801 ctcgtaattt tttccaaacc agcaatcatt ctcagcaaag tagattttcc tgaaccagat
     4861 ggtcccagta acacaattcg ttctccagcg gccacagcta aatcaatttc ttttaaaatg
     4921 gtatgttctc catatttttt tccgatattt cttaattcaa gcgtttctcc catgatttgt
     4981 cctccaattt atttaatacc tgcagaaaca aaagtcccga taatatattt ttgaaaaatt
     5041 gcatacaaga ctaacggtac aaacattgta attaaactaa tcgccattgc tacggtaaaa
     5101 tttgtcccac cttcagaact aatataggtc tgcatcgcca acggcaaagt ataattatct
     5161 actgtccgta aaattaatac cggccaaaca tactcattcc aagaggtaat aaaaaaccaa
     5221 ataccggtag aagtaatcgc atgtctagta agaggaaaaa caattttact catgacggtt
     5281 gtttgtttga tgttatccaa tgccgccaca tctagaaggc tcactggaat ttcccgcatt
     5341 gtttgcgtca ataaaaaaat gcccatcgca tcagcaaatt gtggtattat cacaccccaa
     5401 gtagtatcaa ttaaattaag ttttgcaatc aacaaataat taggaatcat cgcaacagta
     5461 aaaggaatga acatcgtacc aatgatgata aagtaccaga ttttcttccc tttgattcgg
     5521 caataagtaa aactatatgc cgcaaaaaaa gctacgccta acttaacaac cgttataatt
     5581 gtagcaataa aaaaagtatt tctcgtaatt ttgataattg gaagattttc gaacaaatag
     5641 caaaaatttt ccaaggtaaa cggctgtggt atcaaactag taatcgtctg ataggcatct
     5701 ttcaatggct taaaagaatt ggccataata aacaataatg gatagataat aatgattatg
     5761 atgaacataa ataagagatg tggcaaccag cgtttaattt tcatagtaaa cccccttttc
     5821 cacaaattta aatattaccc ctaagaaaac agcaaatatt aataaagtta aaactgaaat
     5881 tgctgaagat tctccgactt tatacatttc aaaagccttt tgataagcat aatagatcac
     5941 gtttgcacta ccattattcg gtccaccacg agtgatgatc ttaatcggag taaacacata
     6001 ctgcagacct tgaacaatgg tcatcaccaa taaatatagt cctgtcgaac tactcatagg
     6061 caacacaatt gtaaaaaaaa tgcgccagat gggtacacga tccaacttag ctgcttcgat
     6121 catctcttga tcaattccca caattgctgc aaaaatgaca atgaaattat aaccaaaaac
     6181 tttccaagca gtaatacctg caatcacagc aattacccaa ccttgtaaat tcatccacat
     6241 aggcagtctt aaaccaaaat ttcccaacaa gacagcaatt ggtcccgata ccggatttaa
     6301 tatccatgta aaaaccatcc ccgccactac caaagaaatc acagctggaa taaaaaataa
     6361 tggtttatac aaattctgcc ctttttctat aactattcct aataaaaagg atgtaatata
     6421 tgggaacaca cagctcagca agacaaatag aacggtatag gacaaagtat tcttcaacac
     6481 tttatgaaag actggatcag tcagcacctt tacatagttg gcaaagccaa caaattcttt
     6541 agtgggggcc accatattcc agtcaaaaaa actcaaatag atcgtataaa tcaatggata
     6601 aaaaacaaac acgcccaaca aaataattgc tggcgccaga tacaaataac ctacatagcg
     6661 cttcatttcc gacagcctcc tcatccttat gtcaaaagca gacgaccttg tttttccttt
     6721 cttacaggcg ctgtcgtctg cttgctttgc ttatttgagt aactcattaa tttccttttg
     6781 agtagctttc atcgtttcat tgacggaatt acctgataaa attttatccc gcatatccaa
     6841 caatttttgt tcagcttcta atccagcatc tccaggaaat gaactccaac tgaccatctt
     6901 atccatctga tcaattgctg gtatcatcat ctgattttca gctaagaaag cttttaatcc
     6961 attttgggcg tctgcaacat ctttacgtgg tggtaaataa ccagtacctt ctgtccattt
     7021 agctaaagat tcaacactat aaagatattt taagaaatcc caagttgctt cctttttctt
     7081 atccgtttgt gatgtaacag ctaacattgc gccaccagca ggtagtttaa cttttttccc
     7141 ttcccaactt ggtgactcaa ctgctttagc agtaaaagta ctattttttt gaatgttgtc
     7201 cctttgagca atagtggtat agaccatcgc gacgttacca tcaataaagc tttgcacacc
     7261 ttgttcccac gtagtgtgta atgccgtctg gtcttttacc atcctttgat ataattcata
     7321 agcagcgata ccatctttag aagcaaaggt agctttgcca tcttttatta cacttgcccc
     7381 attactttct aaaattgctt gttgtgccca attatcagca ggttcttgaa tatacacgcc
     7441 atactttcca gttttttctt ttattatctt agaaaaagtt tgaacttctt cccaagtttt
     7501 tggaccattt tcatccaagc ccgctgcttt taatatatct tgatttaaat ataaaaccgg
     7561 attactaatg gagtaaggaa tacccacttg tttaccatct ttttcagaca gtgccatgat
     7621 attgtctaaa aaatgatctt taaaataatc agcattatcc ggatccactt cttctactaa
     7681 agattgagga ttagtatatt tgaaattttc tgcaaaataa tctttaaaag cccatcccac
     7741 ttgtactacg tccggtattt ctttggaggc aacagaagct tgtaaatttt gcatcaagcc
     7801 tttatacata tcaggattat attttgctac aaccttcact ttgtcttgag attgattgta
     7861 tttatctact aattcattca ctgtttttcc acctagggta tccgcattaa catgccaata
     7921 ctctatcgtt acacgaccct ccgatttttt tgttgtcttg gtattcccac taccacaagc
     7981 tgccaaagag atgacactta aaacagacac aacccctata acaagatttt tctttaacat
     8041 aacattaact tcctcctaat ttctttatgg ccatttttct aatcaagaaa tattatgaac
     8101 taccaatgta aatttctagt taattttctg taaataattc gaaatttgtg catttcgggg
     8161 tacagggtca ctggacatat ccatcaagct aagaataaag cccccatatg gtcatgtatc
     8221 ccatctccaa atctcttaac tcctctgttt tgattataaa atagtttatt ctgtgctcct
     8281 ttcttccata aataggccca tgcacaacgt gaggttatat tataaacacc acagcgcgca
     8341 aggtactcaa tcgtttctag ttcattgact gtccatacag aaaatgtcaa tcctagctgc
     8401 tgccccactt cacgggccaa cttagttaac catccttcat gaatattgac gatttgaacg
     8461 ctttgatagg ctaatttctg taaggtatcg atcaaatctg cttcggtaat atccccaagt
     8521 tttttccctt cgaactgatt ttcaatatta taaaatatat gctttcccca aatcgcagcc
     8581 caagataatt gtacatagcc actaagaaca atttgtggcc agatttgata ggtttgtgcc
     8641 agcgaaaaaa cggccatttc caatccctct tcttttaaat cacagttaat ggtcatggtt
     8701 gccttctcat tggccactgc ttgaaacacc tgctcaaggg ttaccgaatc tgggacaact
     8761 tcttacctgt catgggctag tattagcgta ccatctgttt gctgacgcac atctacttca
     8821 aaacaaagct ggcgatccaa acaaaagcga agaaatgccc agctattctc ctccgtattg
     8881 tcactaccag agtgtgcagt gatcatggtc attttgaccc ctcctttaat tccgtgattt
     8941 tctcccacac gtccactata ccgatgaagc gttaatttct tgttaatttc ttgtaaattc
     9001 cctgcgtttt ttcagtaaac tagttgtaaa tcttgcatgc tccttcctac gaactcatga
     9061 atcgtcgatt ttgcccttct agctagtttc accaattaag tagttctaca ttccccgctt
     9121 ttaacctact acaccttttt ttgttttcag ctattccttc ttcttgctga aagtccttga
     9181 ggttctttct tacaggatac catcaaggtg ttcaatggct tattcccatt gaatgaccga
     9241 gacttactat cagatttctg ctcgttgaac caagtctttg atacaggatt tcttccgtca
     9301 tcttctcaca aataccacct tgcgtttctc tagcgattgg tcgatgtgca ccctcccagt
     9361 ggactgtcac tacccctttt tgctatgcct agcccacaaa aaaagctgtc ctcacagaca
     9421 g
//
LOCUS       DACDKM010000105         9352 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20834_2016
            SAMN12640370-rid9891663.denovo.105, whole genome shotgun sequence.
ACCESSION   DACDKM010000105 DACDKM010000000
VERSION     DACDKM010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640370
            Sequence Read Archive: SRR10040891
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9352)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9352)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742835.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742835.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:04:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,012
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 29 of 75
            Pseudo Genes (incomplete)         :: 33 of 75
            Pseudo Genes (internal stop)      :: 19 of 75
            Pseudo Genes (multiple problems)  :: 5 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9352
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640370-rid9891663.denovo.105"
                     /isolate="E20834_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(93..761)
                     /locus_tag="IV334_002314"
     CDS             complement(93..761)
                     /locus_tag="IV334_002314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HAP8944363.1"
                     /translation="MKKLNLFTELCGIILGAGIYGLAVTGINIPSKLADGGVTGIALL
                     LHNLFGFAPSVTSLLFNLPLLLISLFLFGKHAFLRTIVGTFALVFFLHLWESLHIHFA
                     VDSLLINSLMTGILSGIGCGLVFRFGGSTGGTDIIYQAIEKYFHIKIGKSLFLITFGI
                     LVVSLLYLDLTHFLYTLLSCSILSYTLNKVKYLEIGSVLPQLEAKQTTETFEPLDPLE
                     QLEH"
     gene            895..1182
                     /locus_tag="IV334_002315"
     CDS             895..1182
                     /locus_tag="IV334_002315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8944364.1"
                     /translation="MRAADLLTILTKSPKKTEYQGVFLAQEQKLVPVTMVQINKANQF
                     IFLFEHGKAPLAMKDIFLFLMKNREKAVYYQSKEELIPLYGVKEIENKVVI"
     gene            1598..3136
                     /locus_tag="IV334_002316"
     CDS             1598..3136
                     /locus_tag="IV334_002316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease/substrate-binding
                     protein"
                     /protein_id="HAP8944365.1"
                     /translation="MNNFIQTLSERKGELLEAAFQHLSISLISLLIAALIAIPLAIWA
                     ANHKKMAEVLLQITGVLQTIPSLALLGLLIPFVGIGTVPALIALVIYALLPIFQNTYI
                     GLAEIDPSIEEAAVAFGMSRMRRLLKVELPIALPVIISGIRTALVLIIGTATLAALIG
                     AGGLGTFILLGIDRNTPVLTLIGAISSALLAIIFSGLIRWLQHKKPRYSIITLAAVVC
                     LFGGVSLYQSALFKNETITIAGKLGAEPEILIQMYKELIEDNTKTEVELKPNFGKTSF
                     LFNALDQGQIDIYPEFTGTVLESLVKVPEAMKSADLTKEKTYDEAEELLNKQFDMTLL
                     KPMAYQNTYALAVKRSFANEHQLKKISDLAAISDQIKAGFTLEFIDRSDGYKGIQEAY
                     GLNFSSVQSMEPSLRYQAINNGDVNIVDAYSTDSQLKEYDLVTLEDDRGLFPAYQGAP
                     LMKKDFAKEHPEIVKALNKLSGKITEDQMIDMNYQVNVEKKKPTEVARIFLKNEGLVK
                     EEDQ"
     gene            3133..4098
                     /locus_tag="IV334_002317"
     CDS             3133..4098
                     /locus_tag="IV334_002317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP8944366.1"
                     /translation="MTKLIEFKHVQKKYDGKYVIDDLNLAIKKGEIFVLVGPSGSGKT
                     TTLKMINGLSKPSAGDIYFKGKSLNEYNLQKMRWNMGYVLQQIALFPTMTVKQNIEVI
                     PEMLGWEKQKRADRVDELLQKVGLSPDIYRDRMPRELSGGEQQRIGIIRAIAASPDVI
                     LMDEPFSALDPISRNSLQELVLSLHEELGTTIVFVTHNMEEAIKLGDRIAFMKDGEII
                     QCDTPEQLLMNPKNDYVRHFFDEPKQTKEWRVEDLVVNGYFLNEIPENARQQVCFDTP
                     MKEVYSLLSVYPSIVIVEKQRSIGSLTSKEIFAFLSREEEGNENI"
     gene            4085..5407
                     /locus_tag="IV334_002318"
     CDS             4085..5407
                     /locus_tag="IV334_002318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HAP8944367.1"
                     /translation="MKTYDAIIIGSGVSGLSAAYGLKEAGKTVLVVEEDLWGGTCPNR
                     GCDPKKVLLSAVEARNRVKQLSGKGFNEIPTANWEELQKFKRTFTDPVPESRKKQLAE
                     AEIDHLSGTARFLDDSSIEVNEEVFHADYLVLATGQRPTILPVEGKEYLKTSADFLSL
                     PVLPKEIIFIGGGYIAFELATIANAAGSKVTIVHHNQRPLKEFEASLVEEAVHQMEAS
                     GIQFAFGVETQKIISEGTRYRLVGKETELVADMIFCATGRQPNTESLALEQANIVFDK
                     HGIAVNDYLQTSNPKIFACGDIVSRKTPKLTPVATFEGNYVAKRITDATSEPIKYPII
                     PTIVYASPKLAEVGVTKSHASSSDQVVEMDLTSWFTYHRVNEPVAKAELTFDQQNYLI
                     GAAVISEQADELIDDLTLVINQKLTKKELDSYIMGYPTLASDLSYLLK"
     regulatory      5470..5574
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            5648..6391
                     /locus_tag="IV334_002319"
     CDS             5648..6391
                     /locus_tag="IV334_002319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP8944368.1"
                     /translation="MKKNLYPAVSMAFILIIWQIVVIVWKIPAFILPGPAAVFSALAA
                     DAQVLLSHSVTTLNEAVLGLVIASVLSFITALAMDYWKFLEMSFYPLLVISQMLPIMV
                     LGPLLTLWFGFGMLPKVILVVLISYFPIVVSFSDTLRKVSKEQIIFLKTMGADTGKIY
                     RIYKIPAGLSGFFSGLKVAATYCVSGAVVGEWLSAQAGLGYYMIRVKNSYQIDKVFAA
                     IICVILLSLLLNGACSLLKKGYYRMLYIF"
     gene            6406..7398
                     /locus_tag="IV334_002320"
     CDS             6406..7398
                     /locus_tag="IV334_002320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAP8944369.1"
                     /translation="MKKLWLLLPLLLLLSACGTAKETSSKQEIKDLKKVTLVLDYVPN
                     TNHTGIYLAKEKGYYKEAGLNVQIIEPGDNSTSIGLVGADKAQFGVSYQEDVTYAHAD
                     GQNIPVKAIATVIKHNTSGFATLSDSNIHSPKDFEGKTYAGWQSPSEEAVLKAVMEKD
                     GGDFSKLTMVGSNGEGPESLGKSSDIQWYFEGWDMIKAKEAGIELNYIPLKELDERLD
                     YYTPVIITNDQLIKSDPELVQSFMDATKKGYQEAIKDPNDSAKLLQKYAKENDRTFLE
                     ESQAFLSKNYTDDPENWGLMEEKVWRNYTAFMQENGLIKQDVPSQELFTNQFIK"
     gene            7410..8147
                     /locus_tag="IV334_002321"
     CDS             7410..8147
                     /locus_tag="IV334_002321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP8944370.1"
                     /translation="MSIQISHLNVVIENLKILEDIHFTIPKDTFTSIVAPSGAGKSTL
                     LKTLTGVQPMTSGTIKVDDQKVTGLHTAFSYMPQEDMLLPWLSVYQNVTLYQKINHLT
                     INEEQVRDYLDIFGLNGYEHFLPEQLSGGMKQRTALLRTIMNPSSYLLLDEPFGALDA
                     MTRGQMQDWMLKLPKKAKRTTLLVTHDIEEAIYLSDRILVLSARPAHVIAEIQVPEKR
                     RSREWLLQQSELKQTIYQLLAGELDVK"
     gene            8137..8859
                     /locus_tag="IV334_002322"
     CDS             8137..8859
                     /locus_tag="IV334_002322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HAP8944371.1"
                     /translation="MLSDAHCHLDGSQSLALLQQEHTILTIINCDSPEEWKENRQLAA
                     SKTQALSYGIHPWKADSYTFEQVEPFLKKARIIGEIGLDNIWTNVPMTTQKKVFERQL
                     AFAAINEKPVVLHTKGCEKEILNYIQAYPNHYLIHWYSSLDYQQDYIDLGCYFSIGLD
                     LKKNPAVWQLAKAVPIDHLLIETDGLHAAEWALGGIFSEKDYVSLLEEQLKTIAEIKE
                     LSVEEVKKNTYQNLERFIRLGK"
     gene            complement(8943..>9352)
                     /locus_tag="IV334_002323"
     CDS             complement(8943..>9352)
                     /locus_tag="IV334_002323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="peptidase M23"
                     /protein_id="HAP8944372.1"
                     /translation="QLTIPTEGSAQAAAEPQAAVQEAPVQAEQPVVQETVQTEKQAAP
                     VAETQPAPAVTETAATPASTSSAKEWIAQKESSGSYTATNGRYIGRYQLDSSYLNGDY
                     SAANQERVAEQYVTSRYGSWEAAKAFWEANGWY"
BASE COUNT         3067 a         1605 c         1969 g         2711 t
ORIGIN      
        1 atttttttat aaagaagatt ttactgaata atatcatttt agtttacata atgatatttc
       61 tgtaaattaa ataaagtagt gaaaaataag aatcagtgct ctaactgttc aagaggatca
      121 agtggttcaa atgtttctgt agtctgtttt gcttccaatt ggggcaaaac agatccaatt
      181 tccagatact tcactttatt caatgtataa gatagaatag aacagctaag taatgtatac
      241 agaaaatgtg tgagatccaa ataaagcaag gagacaacta aaataccaaa agtaatcaag
      301 aaaagacttt tgccaatctt aatatgaaaa tatttttcaa tggcttgata aatgatatct
      361 gttccgcctg tgcttccgcc gaatcgaaag acgagaccgc aaccaatgcc agaaagaata
      421 cctgtcatca aactattgat cagtagacta tctacagcaa aatgtatatg aaggctctcc
      481 cataaatgta aaaagaaaac tagcgcaaat gttccgacga tcgtccggag aaaagcgtgt
      541 tttccaaaaa gaaacaatga gataagcaat aaaggtaaat taaaaagcag tgaagtgaca
      601 gaaggagcga atccaaaaag attatgcaaa agcaaagcaa taccagtaac cccaccatct
      661 gccagcttag atggaatatt gattcctgtc acagctaatc cgtagattcc tgcgcctaaa
      721 atgattccgc acaattctgt aaataaattt aattttttca tttctagcct cttttctttt
      781 gaaaattttc agcaacttct ttattatact gacttatact cttacttcaa gaggaaaatg
      841 aaaaaaaatt atttttggct gtttcttttt atttatcaaa taaggagtgg aaaaatgcga
      901 gcagcagact tattgacaat tcttacaaaa tcacccaaaa aaacagagta tcaaggagtt
      961 tttttggctc aagaacaaaa actggtgcct gtgacaatgg tccagataaa taaagcaaat
     1021 caatttatct ttttatttga acacggcaaa gcaccacttg ctatgaaaga catctttctt
     1081 tttttgatga agaaccgtga gaaagctgtt tattatcagt caaaagaaga actcattcct
     1141 ctttatggag tgaaagagat agaaaataaa gtggtgatat aagtaaaaat aagaaaacaa
     1201 gacgtttatg atgtcaaaac aaccattttt tacatttttg gcaaaaaagt gtcatgatac
     1261 agtataatat tcgttgaaca ggccaataaa ggtgttctcc cccacgagat ttctacttta
     1321 ttggctgttt ctttttatta aaataaaagt tgtgtgtgta aaaaagatat agctgattcc
     1381 aagtgacaat ttcgtataga tttttatggt attcgtagtg aaaaaacgag aaccaaaaag
     1441 ttgatccgac tttgttgctt ggattctttt tttgtcagca tatttagagg tatttttttc
     1501 ttttttttgt tcgtgctgta aggttaaggc aagacaagtt agttgggaaa aaggaaagaa
     1561 atgtttatac ttaaagaata acagaaagga aggtcgcatg aataatttta tacaaacatt
     1621 aagtgaaaga aaaggcgagt tgctggaagc tgcatttcaa catctgtcca tttctctcat
     1681 ttctttgctg attgcagcac tgattgctat tcctttagcg atttgggcag ccaatcataa
     1741 aaaaatggca gaagtcttat tgcagatcac gggtgtgctc caaacgattc cttcacttgc
     1801 cttattaggt ttacttatcc catttgtagg gatcggaact gttccagcat tgattgcact
     1861 tgttatttac gctttgcttc cgattttcca aaacacgtat attggtctag ctgaaattga
     1921 cccgtctatt gaagaagcag cggtagcatt tggtatgtcc agaatgagac gcttgctgaa
     1981 agtagagctt cccatcgcat tacctgtcat tatctcaggt atccgaactg ccttagtttt
     2041 aatcatcggt acggctactt tggcagcatt gatcggagca ggtggattag ggacatttat
     2101 tttgttaggt attgaccgaa atacacctgt tttgacattg attggtgcga tcagttctgc
     2161 cttgcttgca attatattca gcggactgat tcgctggttg cagcataaga aacctcgata
     2221 ttccatcatt actttagcag cagtggtgtg tttatttggc ggcgtttctt tatatcagtc
     2281 tgccttgttt aaaaatgaaa ccatcacgat tgctggaaaa ttaggagcgg aacctgaaat
     2341 cctgatccaa atgtataaag aattgattga agacaacaca aaaacagaag ttgaattaaa
     2401 accgaatttt ggcaagacga gttttttgtt caatgcactg gatcaaggtc aaatcgatat
     2461 ctatcctgag ttcacaggta ctgtcctaga aagcttggtg aaggtacctg aagcaatgaa
     2521 aagtgctgat ttgacaaaag aaaaaacata tgacgaagca gaagaattat tgaataagca
     2581 attcgatatg acattattaa aaccaatggc ttaccaaaat acgtatgcct tggcagtcaa
     2641 gagatctttt gcgaacgaac atcaacttaa aaagatttcc gatttggcag ctatctctga
     2701 tcagatcaaa gcgggattca cactggaatt tattgatcgt tcagatggat ataaaggaat
     2761 ccaagaagca tatggcttga atttttcaag tgtacaaagc atggagccaa gtttgcgtta
     2821 ccaagcaatc aataatggag atgtcaatat tgtggatgcc tattccacag atagccagct
     2881 caaggagtac gatctagtga ctctagaaga tgatcgaggg ttattcccag cttatcaagg
     2941 cgcaccgctg atgaaaaaag attttgctaa agaacatcct gaaatcgtta aagcactgaa
     3001 taaattaagc ggaaaaataa cggaggatca aatgattgat atgaattacc aagtcaatgt
     3061 cgaaaagaaa aagccgacag aagtagcacg aatcttcctg aaaaatgaag gattagtaaa
     3121 ggaggaagat cagtgactaa attaatcgag ttcaaacatg tgcaaaaaaa gtatgatgga
     3181 aagtatgtca tcgatgacct aaatctggca atcaaaaaag gagaaatctt tgttttggtt
     3241 ggtccttccg gtagtggaaa aacaactact ttgaaaatga tcaatggatt atctaaacca
     3301 tcagcaggag acatctactt caaaggaaaa tcgttaaatg aatataatct gcagaaaatg
     3361 cggtggaata tgggatatgt gctgcagcaa atcgctttgt tcccgaccat gacagtcaag
     3421 caaaatattg aagtcatccc tgaaatgtta ggttgggaaa agcagaaaag agcagaccgt
     3481 gtagatgaac ttcttcaaaa agttggctta tctcctgata tttatcgaga tcgtatgcct
     3541 agagagttgt ctggaggaga acagcaacgg atcgggatca tccgagctat tgctgcaagc
     3601 ccagatgtga ttttgatgga cgagccattt agcgcattag atccgatttc aagaaactca
     3661 ttacaagaat tggttttgtc cttacacgaa gaattgggca cgaccatcgt tttcgttacg
     3721 cacaatatgg aagaagcaat caagctaggt gatcgtattg catttatgaa ggatggagaa
     3781 attatccaat gtgacacacc agagcagtta ttgatgaatc ctaaaaacga ctacgttcgg
     3841 catttctttg atgaaccgaa acagacgaaa gagtggcgag tagaagattt agtcgtcaat
     3901 ggatatttct taaatgaaat accagaaaat gctcgtcaac aagtttgctt tgatacgcct
     3961 atgaaagaag tatattcgct tctttcggtg tatccaagca ttgtgatagt agaaaagcaa
     4021 agaagtattg ggtcgctgac aagtaaagaa atctttgctt ttttaagtcg agaggaggaa
     4081 ggaaatgaaa acatatgatg caatcatcat cggttcaggt gtgagcggct taagtgctgc
     4141 ttatggattg aaagaagcag gtaaaacagt actagtggtc gaagaagatc tatggggcgg
     4201 gacttgtcct aaccgtggat gtgatcctaa aaaagtcttg ttaagtgcag tagaggcacg
     4261 taatcgggta aaacaattga gtggaaaagg ttttaacgag atcccaactg ccaactggga
     4321 agaacttcaa aaattcaaac gaacatttac ggatccagtc ccagaaagcc gaaaaaaaca
     4381 attagcagaa gctgaaatcg atcatctttc aggaaccgct cgttttttgg atgattcatc
     4441 tattgaagtc aacgaagaag tcttccatgc ggattactta gttttggcaa ccgggcaaag
     4501 acctacgatc ttaccagtgg agggaaagga atacttgaaa actagtgcag actttttatc
     4561 attgcctgta ctgcctaaag aaatcatttt tatcggtggt ggttatattg cttttgagct
     4621 agctacgatt gccaatgctg cagggagcaa agtaaccatt gtccatcata atcaacggcc
     4681 attaaaagaa tttgaggcgt ctttggtaga ggaagccgtc catcaaatgg aagcatctgg
     4741 cattcagttt gcttttggtg ttgaaaccca aaagatcatt tcagaaggta ctcgctatcg
     4801 tttagtcgga aaagagacgg aattagtagc agatatgatt ttttgtgcga ctgggagaca
     4861 gccgaataca gaatctttag cacttgagca agcgaatatc gtctttgata aacacggaat
     4921 tgcagtcaat gattatctgc aaacgagcaa tccaaaaatt tttgcgtgtg gagatattgt
     4981 ttcaaggaaa accccaaaat tgacaccagt cgccactttt gaaggaaatt atgtagctaa
     5041 gcggataacg gatgcaacaa gcgaacctat caaatatccg attataccta cgatcgtcta
     5101 cgccagtcct aaacttgctg aagtaggagt tacaaaaagt cacgcctctt cgtcagatca
     5161 agtggtagag atggatctta ccagttggtt cacctatcat cgtgtcaatg aaccagttgc
     5221 taaggctgaa ttgacctttg accagcaaaa ttatttgatt ggtgctgcag tgattagcga
     5281 gcaagcagac gaattgatcg atgatttgac actcgtcatc aatcaaaaat tgactaaaaa
     5341 agagttggat agctatatca tggggtatcc gacattggca agtgatctct cctatttatt
     5401 gaaataatca gcatgataac taaagcgctt gttgcttttt ccttcttgtg ctacacttta
     5461 aaaaatgaat acctgtttct ggggatgtcg tagacgacat gagagtaagt tgtgaactta
     5521 gacccattga acctgtcagt taatactggt gaagggagaa acatagcaga cgcttgaagc
     5581 gggttactat gtcgtgccca tttcaagcgt tttttgttat tcatattaga agaaaggagg
     5641 taacaaaatg aagaaaaatc tttatccagc agtcagtatg gcattcattt taattatttg
     5701 gcagattgtt gtcatagttt ggaaaatacc tgcctttatt ttacccggac ctgctgctgt
     5761 attttctgca ttagcggcag atgcacaagt attactttcc cattctgtca cgacattaaa
     5821 tgaagcagtg cttggtttgg taatagctag cgttctttct tttattactg ccttggcaat
     5881 ggattattgg aagtttttag aaatgtcttt ttatccgtta ctagtgattt cacaaatgtt
     5941 gccaatcatg gtgttagggc cattacttac cctatggttt ggtttcggta tgcttccaaa
     6001 agtgatcctt gttgtcttga taagctattt tccgatcgta gtttcttttt cggatacatt
     6061 gaggaaagta tcaaaagagc aaatcatctt tttgaaaacg atgggggcag atacagggaa
     6121 gatttatcga atatataaaa taccagcggg tttatccggc ttcttttccg gtttaaaagt
     6181 ggctgctact tattgtgtca gcggagcagt cgttggagaa tggctaagcg ctcaagcagg
     6241 cttagggtat tacatgatac gcgtaaaaaa cagctaccag atcgataaag tttttgctgc
     6301 gatcatttgt gtcattctat tgagtttatt gctgaatggc gcttgttcat tattgaaaaa
     6361 agggtattat cgtatgcttt atatttttta agagaagggg atcaaatgaa aaaattatgg
     6421 cttttattac cgctactgtt gcttttatcc gcttgtggca ctgcaaaaga aacaagtagc
     6481 aaacaagaaa taaaggattt gaaaaaagtg acacttgttt tggattatgt accgaatact
     6541 aatcatactg gcatctacct agcaaaagaa aaaggctatt ataaagaggc tggtttaaat
     6601 gtccaaatca tcgaaccagg agataattcg acaagtattg gtctagtagg tgcggacaaa
     6661 gcacaatttg gtgtaagtta tcaagaagat gtcacttatg ctcacgcaga tggacagaat
     6721 ataccagtca aagcaattgc tacagtgatc aaacataata cttctggatt cgccacccta
     6781 tcagacagta acatccattc accaaaagat tttgaaggaa aaacatatgc aggttggcaa
     6841 agtccaagtg aagaagctgt cttaaaagct gtgatggaaa aagacggcgg ggacttttca
     6901 aaactgacaa tggtaggaag caatggagaa gggccagaaa gcttaggaaa atcctcagat
     6961 atccaatggt actttgaagg ttgggatatg atcaaagcaa aagaagcggg tattgaacta
     7021 aactatatcc cattgaaaga attagatgaa cggttagatt actatactcc ggtaattatt
     7081 acgaatgatc aactgatcaa aagtgatcca gaattggtac agtcttttat ggatgccacg
     7141 aaaaaaggct atcaggaagc tattaaggat ccaaatgaca gtgctaaact gttgcaaaaa
     7201 tatgcaaaag aaaatgatcg gacgttttta gaagaatcac aagcattctt gtcaaaaaat
     7261 tatacggatg atcctgagaa ttggggattg atggaagaaa aagtttggcg caattataca
     7321 gcttttatgc aggaaaacgg attgatcaag caagatgtac cttcgcaaga actattcact
     7381 aatcagttta taaaatagga gtgatagata tgtcaatcca aatcagccac ttgaatgtag
     7441 tcattgaaaa cctgaaaata ttagaagaca tccatttcac tatccctaaa gataccttta
     7501 catcgattgt cgcgccaagc ggagccggta agtcaacatt gctgaaaact ttaacaggag
     7561 tccagcctat gacttctgga acgatcaaag tagacgatca aaaagtaact ggtctccata
     7621 cagcatttag ttatatgccg caagaagata tgttacttcc ttggctgagc gtctatcaaa
     7681 acgttacgtt atatcaaaaa atcaatcatc taacaatcaa tgaagaacaa gtaagggatt
     7741 atctggatat ctttgggtta aacggctatg aacatttttt gccggaacaa ttatctggcg
     7801 ggatgaagca gcgaacggcc ttgttacgaa caatcatgaa tccttcttct tacttgcttc
     7861 ttgatgaacc atttggagcg ttagatgcta tgaccagagg gcagatgcaa gactggatgt
     7921 tgaagctgcc taaaaaagca aagcggacta ctttacttgt gacgcatgat atcgaagaag
     7981 caatttattt atcagacagg attcttgtgc tttccgcccg tccggcacat gtgattgctg
     8041 agattcaagt gcctgaaaaa cgacggtcac gtgaatggct actgcagcaa tcagagttga
     8101 agcaaacgat ttatcagtta ttggcaggtg aattagatgt taagtgatgc tcattgtcat
     8161 ttagatggga gtcagagttt agcactcctt cagcaggaac acacgatttt gacgatcatc
     8221 aattgtgatt cgccagaaga atggaaggaa aacagacagc ttgcagcaag taagactcaa
     8281 gccttaagtt acggtatcca tccttggaaa gcagatagct atacgtttga acaagttgag
     8341 ccttttttga aaaaagcaag gatcatcggt gaaatagggt tagataatat ttggacgaat
     8401 gtaccgatga ctacacaaaa aaaagtattt gagcgacagc ttgcttttgc agccataaat
     8461 gagaaacctg ttgtactgca cacaaaagga tgcgaaaaag agatcttaaa ttatatccaa
     8521 gcttacccta atcactattt gatccattgg tattcttctt tggactatca acaagactat
     8581 attgatttag gctgttattt cagcattggt cttgatttga aaaagaatcc agcagtctgg
     8641 caactagcta aagcagtccc aatcgaccac ttacttattg aaacggatgg actacatgca
     8701 gcagagtggg ctttaggggg gattttttca gaaaaagatt atgtctcact gcttgaagaa
     8761 cagttaaaga caatcgctga gatcaaagag ctatcagttg aagaagttaa aaaaaatact
     8821 tatcaaaact tggaacgttt cattagatta ggtaaataaa aaagagatgt gacacaacta
     8881 gtgtcgcacc tctttttggc tttctttgta taaaagtaaa ctgtttgatt aataaatgaa
     8941 tcttagtacc aaccgtttgc ttcccagaat gctttcgccg cttcccaaga accataacgt
     9001 gaagtaacat attgttctgc tactctttct tggtttgcag cagagtagtc accatttaag
     9061 taagaagaat ctaattggta acgtccgatg taacgtccgt tcgttgctgt gtaagaaccg
     9121 cttgattctt tttgtgcgat ccattctttt gcagagcttg tgcttgctgg agtagcagca
     9181 gtttctgtta cagcaggagc aggttgtgtt tcagcaacag gcgctgcttg tttttccgtt
     9241 tgaaccgttt cttgtacaac aggttgttca gcttggactg gtgcttcttg aacggctgct
     9301 tgaggttcag cagctgcttg tgcagagcct tctgttggga ttgttaattg tt
//
LOCUS       DACDKM010000106         8919 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20834_2016
            SAMN12640370-rid9891663.denovo.106, whole genome shotgun sequence.
ACCESSION   DACDKM010000106 DACDKM010000000
VERSION     DACDKM010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640370
            Sequence Read Archive: SRR10040891
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8919)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8919)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742835.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742835.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:04:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,012
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 29 of 75
            Pseudo Genes (incomplete)         :: 33 of 75
            Pseudo Genes (internal stop)      :: 19 of 75
            Pseudo Genes (multiple problems)  :: 5 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8919
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640370-rid9891663.denovo.106"
                     /isolate="E20834_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..776
                     /gene="menA"
                     /locus_tag="IV334_002324"
     CDS             <1..776
                     /gene="menA"
                     /locus_tag="IV334_002324"
                     /EC_number="2.5.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoate
                     polyprenyltransferase"
                     /protein_id="HAP8944373.1"
                     /translation="YMDYEKAHSEVYKYEENVIGRRNLSPVLIRNMILAMIAFVFVIG
                     VILTINTGWLLLLMGFICCFIGVFYTFGPIPLSRMPLGEIFSGVTMGLGIFAMVVYVN
                     TFGSDVYDLTVNFSSGQFDLVGNLWAIAAIVLASLPLVFTIANIMLANNLRDLDRDIE
                     NHRYTLVFYIGRPAGTVLFQLLMYACYFVILMGLLSGIYEWPILVTFLTLPIVKKHLD
                     QFKQTLPQPISFSYSIKNMVLFNASYVLGLLLTIIMQAI"
     gene            complement(1711..3057)
                     /gene="gor"
                     /locus_tag="IV334_002325"
     CDS             complement(1711..3057)
                     /gene="gor"
                     /locus_tag="IV334_002325"
                     /EC_number="1.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-disulfide reductase"
                     /protein_id="HAP8944374.1"
                     /translation="MQIYDYIVIGGGSGGIASANRAGMHGAKVLLIEANELGGTCVNV
                     GCVPKKVMWQASTILETIERDASSYGIQADLKNVDFAGLVERREKYIDFLHGAYQRGL
                     DSNHVEAIKGYATFIDSQTIEVNGETYRAPKILIATGGRASKMTIPGGEYAIDSNGFF
                     ELTELPKRAIVLGAGYIAAELSGVFRGLGSEVMWAYRKERPLRTFDKMLSDNLIQMYQ
                     EAGIKTYAHHIAKEITKNNDEYTVLFENGETLTGDCVLFAGGRVPNTEKLGLENTNVE
                     LDKKGFIKVDKYQNTTDEHIFAVGDVIGKLDLTPVAIAAGRRLSERLFNGKKDSYLDY
                     KLVPTVVFTHPPIATIGLTEEEALEKYGENELKVYRSRFTPMYFALNDYRQKCEMKLI
                     CVGKEERIVGLHAIGVGVDEMLQGFAVAIKMGATKEDFDNTVAIHPTGAEEFVTMR"
     gene            complement(3191..4432)
                     /locus_tag="IV334_002326"
     CDS             complement(3191..4432)
                     /locus_tag="IV334_002326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HAP8944375.1"
                     /translation="MTVKRKLKRPEILAPAGTLEKLKTAIHYGADAVYIGGNAYGLRS
                     RAGNFTYEEMAEGVAFAKEHDAKVYVAANMVTHEGNEDGAGEFFRSLRDVGISAVIVS
                     DPALIEICATEAPGLPIHLSTQASATNFETLEFWKEEGLERVVLAREVSMEEVAEIRK
                     NTDTEIEAFIHGAMCISYSGRCTLSNHMSMRDANRGGCSQSCRWKYGLFDMPFGQERN
                     SLTDTGEVEEEFSMSAVDMSMIEHIPELIENGVDSFKIEGRMKSIHYVSTVSNVYKAA
                     VDSYMEDPENYVCKQEWIDELWKVAQRELATGFYYNTPTENEQLFGERRKIPVYKFVG
                     EVLSYDEATQIATIRQRNLFSVGDEIEFYGPGFSHFSQTVEVMHNEEGESIDRAPNPM
                     MILTMPVAQPVKPGDMIRKRK"
     gene            complement(4450..5373)
                     /locus_tag="IV334_002327"
     CDS             complement(4450..5373)
                     /locus_tag="IV334_002327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HAP8944376.1"
                     /translation="MIELITTVETIEQAEQLLAAGADTLYFGEEQFGLRLPASFTREE
                     QRQLVELAHKYGKKANIAVNGIIHPEKMKNVPEYLAFLKEINVDVITVGDTGIIYTMK
                     KNPELRIPYIYDAETLVTSARQINFWAKKGAVGAVLAREVPFEEMKAMEEKLDIPVET
                     LVYGATCIHQSKRPLLQNYYNYTKQDEQKDRERGLFISEPKKEETHYSIYEDSHGTHI
                     FASNDLNLSNELNELYQHHYCTWKLDGLFTPGEKFVNITRLFAEAKKQILEGSWDQEK
                     AEKMTSEISKLHPENRELDTGFFYIDPSAIK"
     gene            complement(5594..8086)
                     /locus_tag="IV334_002328"
     CDS             complement(5594..8086)
                     /locus_tag="IV334_002328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HAP8944377.1"
                     /translation="MEELFTERAQAVLEIAQEEAKRLKHQSVSSEHVLLALVVEQNGI
                     AGKVLREMDLNETEIYEEIEHLIGYGGIRSYPKGVFLPYSPRMKQVFALASSEVKKTG
                     SQKVGTEHILMSLLKDESIMATRIMINLGISLSKARQVLKQKMGLAGDSAGKGMNRRR
                     NVNVQDKRQTPQGTPTLDSLARDLTKLAKENKLDPVVGRSREVKRLIQILSRRTKNNP
                     VLVGEPGVGKTAIAEGLAQKIILGEVPEDMQEKRLMMLDMGSLVAGTKYRGEFEDRMK
                     KVIDEIYNDGQVILFIDELHTLIGAGGAEGAIDASNILKPALARGELQTIGATTLDEY
                     QKYIEKDSALERRFARIQVDEPTPEEAEVILQGLRTRYEEHHGVEITDEAVRAAVNLS
                     VRYITSRQLPDKAIDLIDESAAKVRLDQTDHLTKSTVIKLEIDELVQEKEAAIQKQDF
                     ENAAQLRRQEKALRKKLQKVSAIEAKQEQGYSDRVTEEDVATVVSEWTGVPLQQLEKK
                     ESERLLELEGLLHERVVGQDEAVKAVSRAIRRARSGLKDPDRPIGSFMFLGPTGVGKT
                     ELAKALSEVMFGSEDALIRVDMSEFMEKYSTSRLIGSPPGYVGYDEGGQLTEKIRQKP
                     YSVILLDEVEKAHPDVFNLLLQVLDDGHLTDSKGRKVDFRNTIMIMTSNIGATQIREE
                     KNVGFNVQDVTKDHKAMQKRILEELKKAFRPEFLNRIDETVVFHSLSKDEIHTIVQIM
                     SKAIIKRLKEQDIQVKITPSAIDVIGKAGFDPEYGARPIRRALQKEIEDRLSEALLGG
                     EIRLGDHVTVGASKGKITLNVRGPKKETVGSL"
     gene            complement(8093..8560)
                     /locus_tag="IV334_002329"
     CDS             complement(8093..8560)
                     /locus_tag="IV334_002329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CtsR family transcriptional regulator"
                     /protein_id="HAP8944378.1"
                     /translation="MAHQNTSDLIESYIKKILENSEMIEIRRIEMADLFNCVPSQINY
                     VINTRFTIQRGYLVESKRGGGGYIRIAKVRISDKKQLLEQINQLFDDTISEKNAFAII
                     QKLYEDQIITKKEGNLMLSAIAKNTLNFNEYEDHTRARILRAFLERLSYEDGK"
     gene            complement(8696..8769)
                     /locus_tag="IV334_002330"
     tRNA            complement(8696..8769)
                     /locus_tag="IV334_002330"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(8733..8735),aa:Arg,seq:acg)
     gene            complement(8827..8900)
                     /locus_tag="IV334_002331"
     tRNA            complement(8827..8900)
                     /locus_tag="IV334_002331"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(8864..8866),aa:Pro,seq:tgg)
BASE COUNT         2478 a         1884 c         1485 g         3072 t
ORIGIN      
        1 attacatgga ttatgaaaag gctcattctg aagtatataa gtatgaagaa aatgtcattg
       61 gccgcagaaa tctatctcct gtattgattc gaaacatgat tttagctatg attgcatttg
      121 tttttgttat cggcgttatt ctaacgatca atacgggttg gcttttactt ttgatgggat
      181 ttatctgttg ttttattgga gtattctata cattcggacc gattccttta tctcgaatgc
      241 ctttagggga aattttcagt ggggtgacta tgggccttgg aatttttgcc atggtcgttt
      301 atgtcaatac attcggttca gatgtctatg atctgacagt caacttttca tctggacaat
      361 ttgatttggt aggaaatctt tgggctatag cagcaatcgt attagcttcc ttacccttgg
      421 tgtttacgat tgccaatatc atgttggcta ataatttgcg ggatcttgac cgtgatattg
      481 aaaatcatcg ctatacacta gttttttata tcggacgtcc tgcgggaaca gtcttattcc
      541 agctattgat gtatgcttgc tatttcgtga ttttaatggg gttgttgagc ggtatctatg
      601 aatggccgat cttggtcacc ttcttgacct tgccaattgt gaagaagcat ttggatcaat
      661 tcaagcaaac gttaccccag cctatcagtt tcagctattc catcaaaaat atggtcttat
      721 tcaatgccag ttatgtattg gggctattac taacaatcat tatgcaagcc atttaacagc
      781 atgaagaggt tgtgaaaaaa ctgccctgca tcaagtaata agaacagcca aataaaaata
      841 gctccttata tttttcgttt ggttgggctt attaccgcag atgcaagctt ttgaacaccg
      901 tttatcagga ggttgtgaaa aagtcgctta gtttcgagta ttaagaaaaa ttttgtgaaa
      961 atggctttcc acatttttgc ataaatttgg cttaataccg agaaactggc ttttgaacac
     1021 cgtttatcag aggttgtgaa aaagtcgctt agtttcgagt attaagaaaa attttgtgaa
     1081 aatggctttc cacatttttg cataaatttg gcttaatacc gagaaactgg cttttgaaca
     1141 ccgtttatca gaggttgtaa aatcagtgtt ttgtcctgag tattagagaa acaaatggaa
     1201 aaatagttcc tcatattttg ccgttttttg agctaatacc gaaggacagc tgattgaaca
     1261 ctatttgttc ggaaaaagtg actaactatg acacaatttt tttcacagtc ccttttttca
     1321 cttgatttga ttcgtaccag cgaacttgac gagaactttc ttggtttggt aactatatgg
     1381 tattctttta acatcctcgt ttgcttcttc taatttattt atacaaagtc acactttttt
     1441 aaacatctgt tcttcgatac atttctattt cttttaaaca tcgataccaa tattttgaac
     1501 ttaagagtgc ataccaaatg gattcattta ctcttcgtca gttacgaaga taatcaaaat
     1561 ctccaacttc cctacttatt tcgttttgag aattatcgaa gcaatcttac ataaataact
     1621 aaaaaacaaa aaaccagaaa atcagtatac tgattttctg gttttacatg gtttgttcaa
     1681 taaaaagctg ttggaactgt taatattaaa ttatcgcatc gttacgaatt cttccgctcc
     1741 tgttggatgg atggctactg tattgtcaaa gtcttcttta gttgcaccca tcttgatagc
     1801 cacggcaaaa ccttgcagca tctcatcgac accaacacca atagcatgta agccgacgat
     1861 tctttcttct tttccgacac agatcaattt catttcgcat ttttggcgat aatcatttaa
     1921 tgcaaagtac attggggtaa agcgtgaacg ataaactttc aattcatttt cgccatattt
     1981 ttccaatgct tcttcttctg tcaaaccaat tgttgcaatt ggtggatgcg taaagacgac
     2041 tgtcggaaca agtttatagt caagataaga atctttcttt ccattaaata aacgttctga
     2101 taaacgtcta ccagctgcga ttgcaacagg agtcagatct aatttaccga tcacatcacc
     2161 aacagcaaaa atatgttcat ccgttgtatt ttggtattta tctactttga taaacccttt
     2221 tttgtccaat tccacatttg tattttccag acctaatttt tcggtattcg gtacacgtcc
     2281 accggcaaaa agtacacaat caccagttaa ggtttcacca ttttcaaata gcaccgtata
     2341 ttcgtcattg tttttagtaa tttctttagc aatatgatgt gcgtaagttt ttattccagc
     2401 ttcttggtac atttggatca aattgtccga aagcatttta tcaaacgttc taagtggtcg
     2461 ttcttttcga tatgcccaca tgacttcact acctaaacca cgaaatacac ctgaaagttc
     2521 agccgcaatg tatccagcac ctaaaacgat tgctctctta ggtaactctg ttaattcaaa
     2581 aaatccattt gaatcaattg cgtattctcc accaggaatg gtcattttac ttgcacggcc
     2641 tccagtagcg atcaagattt taggagcacg ataggtttca ccgtttactt cgatcgtttg
     2701 actatcgata aaagtagcgt aaccttttat agcctcgaca tgattgctat ctaatcctct
     2761 ttgataagca ccgtgtagaa aatcaatata tttttctcgt ctttctacta gaccggcgaa
     2821 atcaacattt tttaaatctg cttggatacc gtaacttgag gcatctcgtt caattgtttc
     2881 caaaatcgta cttgcttgcc acataacttt tttaggtaca caaccgacgt tgacacatgt
     2941 tccgcccaat tcattcgctt caatcagcaa aacttttgcg ccatgcatcc ctgctctgtt
     3001 tgctgaagca ataccgccag agccgccacc aattacgatg taatcatata tttgcatttt
     3061 gttcctcctt attgaagaga tcaatcaaaa cctttattta acgatccttt ttctacagaa
     3121 aacaagtgta acacttttct tgaattttgt atgcgaagat gcttaaaaca gggatatatc
     3181 agctttttcc ttatttacgt tttcggatca tatctccagg tttcaccggc tgtgcgactg
     3241 gcatagttaa aatcatcatt ggatttggtg ctcggtcaat ggattcgcct tcttcattat
     3301 gcataacttc aacggtttga ctgaaatgac tgaatcctgg accataaaat tcgatttcat
     3361 ctcctacaga aaacaagttt ctctgacgta tcgtagcaat ctgtgttgcc tcatcataag
     3421 ataatacttc accgacaaat ttatatactg ggattttacg acgttcaccg aataattgtt
     3481 cgttttcagt tggtgtattg tagtaaaaac ctgtagctaa ttctcgttgt gctactttcc
     3541 ataactcgtc gatccattct tgcttgcaga catagttttc aggatcttcc atataactgt
     3601 ctacagccgc tttatagaca tttgacaccg tagatacata atggatcgat ttcattcttc
     3661 cttcgatctt gaagctgtct acgccgtttt caatcaattc tggaatatgt tcgatcattg
     3721 acatatccac tgcactcatc gagaattctt cttccacttc gcctgtatca gttaaactgt
     3781 ttcgttcttg accaaaaggc atatcgaaca atccatactt ccagcgacaa gattgagaac
     3841 atccgccacg gtttgcatcc cgcatcgaca tatggttgga caatgtgcat cgaccagaat
     3901 aagagataca catggcacca tggataaatg cttcgatttc ggtatccgta tttttacgaa
     3961 tctctgccac ttcctccatt gaaacttcac gagctaaaac gacacgttcc aacccttctt
     4021 ctttccagaa ttccaatgtc tcaaaattag ttgctgaagc ttgagtggat aaatggatcg
     4081 gcagacctgg ggcttctgtc gcacaaattt cgatcaaagc tggatcagaa acaatcactg
     4141 cagaaatacc gacatcacgc aaactgcgga aaaattcccc tgctccatct tcatttcctt
     4201 catgggtgac catattagca gcaacgtata ccttcgcatc atgttctttt gcaaaagcaa
     4261 ctccttcagc catttcttca taagtaaaat tacctgcacg gcttcttaaa ccatatgcat
     4321 tcccgccaat atatactgca tctgctccat aatggatcgc ggtttttaac ttttccagtg
     4381 ttcctgctgg cgctaggatt tctggtcgtt tcaattttct ttttactgtc atttctttca
     4441 tcatactcct tatttgatag cagaaggatc aatataaaag aatcctgtat ctaattcacg
     4501 gttttcagga tgcaatttac tgatttcgct agtcattttt tccgcttttt cttgatccca
     4561 gctaccttct aaaatttgtt tttttgcttc ggcgaatagt cgagtgatgt ttacgaattt
     4621 ttcgcctgga gtaaataatc cgtccaattt ccatgtacag taatgatgct gataaagttc
     4681 attcaattca ttcgagagat tcagatcatt gctggcaaaa atatgtgtgc catgactatc
     4741 ttcataaata gaataatgtg tctcttcttt tttaggttcc gagataaaaa gacctctttc
     4801 acgatctttt tgttcgtctt gtttcgtata gttatagtaa ttctgtagca gtggacgttt
     4861 ggattgatgg atgcatgtag caccatacac aagggtttct acaggaatat cgagtttttc
     4921 ttccatcgcc ttcatctctt caaaaggaac ttcacgagca agtactgctc caactgcgcc
     4981 ttttttcgcc cagaaattga tttgacgtgc gctcgttact aatgtttcag catcataaat
     5041 atacggtata cgcaattcag gatttttttt catcgtataa ataatacctg tatcaccaac
     5101 tgtgatcaca tctacgttta tttcttttaa aaaagccaaa tattcaggta catttttcat
     5161 tttttcagga tgaatgatcc catttacagc aatattcgct tttttaccat acttgtgtgc
     5221 taattctacc aattgtcgct gttcttctcg tgtaaatgaa gcaggcagac gcaatccaaa
     5281 ttgctcttca ccgaaatata gtgtatctgc acctgcagca agcaactgtt ctgcttgttc
     5341 aatagtttct actgtggtta tgagttcgat cataattttc tcctcctaca acttcataat
     5401 agtagcatag ctctaaaaac tttccaatac ttcttctatt taaagtcgga tttttgaaaa
     5461 ttatttcaca aaaaatcaaa ataaagttcg atatattctg tccaaatata aaaagagatt
     5521 gtgacaaaaa tcttgtcaca acctcttaat caagtcttac tattataaaa attcagctac
     5581 acaggccaag taactatagg gaacctactg tttccttttt aggtccgcga acattcaatg
     5641 tgatcttacc ttttgaagca cctactgtta cgtggtcacc taaacgaatt tctccgccaa
     5701 gcaaagcctc gctcaatcga tcttcaattt ctttttgaag tgcgcgacgg attggacgtg
     5761 caccatattc aggatcaaat ccagctttcc cgatcacatc gattgcagat ggtgtgattt
     5821 tcacttgaat atcttgttct ttcaatcgtt tgataatcgc tttgctcatg atttggacga
     5881 ttgtatggat ttcatcttta ctcaatgaat ggaagacgac agtctcatcg attcgattca
     5941 agaattcagg tcggaaagct ttctttaact cttctaagat acgtttttgc atggctttgt
     6001 gatctttagt aacatcttgg acattgaatc cgacattttt ttcttctcga atttgtgtcg
     6061 ctccgatatt tgaagtcata atcatgattg tatttctaaa gtccactttt ctacctttag
     6121 aatctgttaa atgaccatca tccaatactt gcagaagtaa attgaaaacg tctggatgtg
     6181 ctttctccac ttcatctaag agaatcactg aatatggttt ttgacgaatt ttttctgtca
     6241 actgtccacc ttcatcatac cctacgtatc ctggaggaga cccgattaat cggcttgtac
     6301 tgtatttttc cataaattct gacatatcaa cacggatcag ggcatcttca cttccaaaca
     6361 tgacttctga aagtgctttg gccaattctg tttttccgac accagttggt ccaaggaaca
     6421 tgaatgaacc aattggacga tcaggatctt tcaatccgct tcgtgcacgg cggatcgcac
     6481 gtgaaactgc tttgacagcc tcatcttgac caacgacacg ttcatgcaga agtccttcca
     6541 gttctaacaa gcgttcgctt tcttttttct ccaactgctg tagagggaca ccagtccact
     6601 ccgatacaac tgtagcaaca tcttcttctg ttacacgatc agaataacct tgctcctgtt
     6661 ttgcttcaat tgcagaaact ttttgcagct ttttacgtag tgctttttct tgtctgcgta
     6721 attgagcagc attttcaaag tcttgttttt ggatcgctgc ttccttttct tgtactagtt
     6781 catcgatttc tagtttgatc acagttgatt ttgttaagtg atcggtttga tccaaacgga
     6841 ctttcgctgc tgattcatcg atcaaatcaa ttgctttatc gggtagttga cgagaagtga
     6901 tataacgaac agaaagattg actgctgctc gtacagcttc atctgtgatc tccactccat
     6961 ggtgctcttc atatcttgta cgtaatcctt gaagaatcac ttctgcttct tcaggagttg
     7021 gctcatctac ttgaatgcga gcaaaacgtc gctctaatgc agaatctttt tcgatatatt
     7081 tttgatattc atctagggtc gttgctccga tcgtttgtaa ttctcctcga gctaacgccg
     7141 gtttcaagat attcgaagca tcaattgctc cttctgctcc acctgcacca attaaggtat
     7201 gcaattcatc aatgaataag attacttgac catcattata gatttcgtcg atgacctttt
     7261 tcattcgatc ttcaaattca ccgcgatatt tcgtaccagc aaccaaagat cccatatcaa
     7321 gcatcatcag gcgcttttct tgcatgtctt ctggcacttc acctaaaatg attttttgag
     7381 ctaacccttc agcaatggct gttttgccta caccaggttc acccaccagt acagggttat
     7441 tctttgtccg tcggctaaga atctggataa gccgtttcac ttctctcgaa cgaccaacaa
     7501 ctgggtccag cttgttttct tttgctaatt tcgtcaaatc acgtgctaat gaatctagcg
     7561 taggtgttcc ttgcggtgtt tgccgtttat cttgtacatt tacatttcta cgacgattca
     7621 ttccttttcc agcagaatca ccagctagcc ccattttttg tttcagaact tgacgggctt
     7681 ttgaaaggct gatacctaag ttgatcatga ttcgagtagc catgatactt tcatctttta
     7741 ataagctcat caaaatatgt tctgttccaa ctttttgaga accagttttc ttcacttcgc
     7801 tgctagctaa agcaaaaact tgtttcattc ttggtgaata tggaagaaag actcctttag
     7861 ggtaagaacg gataccacca taaccaatca gatgttcaat ttcttcataa atttccgttt
     7921 cattcaaatc catttcacgt aatacttttc cagcaattcc attttgctcg accactaagg
     7981 ctagtaaaac atgttctgaa ctaactgatt gatgtttcaa ccgttttgct tcttcttgtg
     8041 caatttctaa tactgcttgg gctctttcag taaaaagttc ttccatctaa tctcacttcc
     8101 catcttcata acttaaacgt tccagaaatg ctcggagaat ccgagcacgt gtatgatctt
     8161 catattcatt gaaattcaat gtgttttttg caattgcact caacatcaag tttccttctt
     8221 tttttgtaat tatttggtct tcataaagtt tttgaataat agcaaaagca tttttctcac
     8281 tgattgtatc atcaaataac tgatttatct gctcaagtaa ttgtttttta tctgaaatcc
     8341 ttacttttgc aattcgtata taaccaccac caccacgttt gctttctact agataaccac
     8401 gttggatggt aaaacgggta ttaatcacat agttgatttg tgaaggaaca cagttaaaga
     8461 ggtctgccat ttcaatcctt cgaatctcga tcatttcact attttccaga attttcttta
     8521 tatatgattc aattaaatcc gaggtattct gatgtgccat cttattcatt ccctttgact
     8581 aatattgacc ttaattataa cgaatttgat aatagaaaga aagaagaacg ctttttgtta
     8641 aataaatcct ttagaatcaa caaaaaaaaa tacggaatat actattccgt atcgacgcgc
     8701 ccaagaggag tcgaacccct aaccttttga tccgtagtca aacactctat ccagttgagc
     8761 tatgggcgca tattcaattt aaaaaaatta taaatcattt gaaaacaaaa agcaagataa
     8821 aaaaaatcgg gaagacagga ttcgaacctg cgaccccttg gtcccaaacc aagtgctcta
     8881 ccaagctgag ctacttcccg tttaaaaaat aaaaaagac
//
LOCUS       DACDKM010000107         8905 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20834_2016
            SAMN12640370-rid9891663.denovo.107, whole genome shotgun sequence.
ACCESSION   DACDKM010000107 DACDKM010000000
VERSION     DACDKM010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640370
            Sequence Read Archive: SRR10040891
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8905)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8905)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742835.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742835.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:04:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,012
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 29 of 75
            Pseudo Genes (incomplete)         :: 33 of 75
            Pseudo Genes (internal stop)      :: 19 of 75
            Pseudo Genes (multiple problems)  :: 5 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8905
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640370-rid9891663.denovo.107"
                     /isolate="E20834_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(305..661)
                     /locus_tag="IV334_002332"
     CDS             complement(305..661)
                     /locus_tag="IV334_002332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase"
                     /protein_id="HAP8944379.1"
                     /translation="MEVKVYGSAHCVTSLKAKQWLEHHQLPYQFVDLEERDLSTEEAE
                     ELCSFEEVQAEQLFATWSEAFHNIAIDLSCVGKKQLIFLCTQHTNLLRRPLIIIDEVL
                     FIGYNEQLMEQLILNK"
     gene            complement(675..1085)
                     /locus_tag="IV334_002333"
     CDS             complement(675..1085)
                     /locus_tag="IV334_002333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HAP8944380.1"
                     /translation="MIKVYTSPSCTSCRKARAWLQANQLEFEEKNIFAEPLTESEIKK
                     ILAATEGGVQDIISTRSKIYEKLGIDFNELTLKQMIVLFKEYPSLLRRPILIDEKRIL
                     IGFNEDEIRSFVPREIRCVARNLIYLQNNNILNS"
     gene            complement(1432..3618)
                     /locus_tag="IV334_002334"
     CDS             complement(1432..3618)
                     /locus_tag="IV334_002334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HAP8944381.1"
                     /translation="MNEKEILRLQKELKRWENKYKKLQQVFRLQEEIIATYEAAYGEA
                     PGLIEEEVQVKESEGNSSFFKMMEKRSYNRFNQEQKAAITYDMKKHLRIIAGAGSGKT
                     QTICAKAVYLMTQKQVDEERILMITFTRNAANELKKRVDNFSQRKTAVHIGTFHSIFF
                     RIYNEICRKFPEVAMQGIQGDFSKDTAQKVNAVLQQLIRKYNLYIFDQYGEKTIASRL
                     DYWQNMNLSVEEMVQLVKEKYDSIDKNARQPISERLYGLLTELQEQKRSQQLLEFNDV
                     LQNLKSALENEEIQHYIGQKYDYLFIDEFQDTNPLQWEIVKRMTKENRIKLIVVGDDD
                     QSIYGFRGSEPAYIKNFEKEYPTKTLFLLTNYRSRAAIVQGANRLISYNKNDRIPKAM
                     IPAQKEAGVMEAYLFSDTKAESQWLIKQIQTFIQKNGKYKESIILYRSASQTQQLVQS
                     LLKTNIPFVLEADSPYEGIFGIKSFQHFYQKIVHWQTALNLQKKNQAYQQLLRQLMAD
                     CYLKKSECDQYFSPKYQNIAVADYILSISPNLKSKSAEIREFEEALRKTDQHQEIYSL
                     VQSYLHLPRIAKEIDQSDQEWIREEVKQHATFRRLQALSQETQATAKELKTRLIAYRQ
                     GKLDALCIQSIHKSKGLSYRNVFLIGCNEGSLPYNGATEKKVIDFNEIKAEPVTTIEE
                     ERRLFYVAMTRARNRLYLCVPQMKNSRKLKVSRFIKETGSRVKKGK"
     gene            3836..4519
                     /locus_tag="IV334_002335"
     CDS             3836..4519
                     /locus_tag="IV334_002335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1275 domain-containing protein"
                     /protein_id="HAP8944382.1"
                     /translation="MQTSFHEGRTIGLLLTFIGGAMDSYTYIQYGAFASAQTGNIILS
                     IIQAFDGEWLSVGKKVLSTLFFFLGILLTKYLIDYFRKKEKHFWRLFVLYYEAVIFFF
                     VSLTPVHEHPALVTILIAFTASIQWVAFDKINGRAYTNLFTTGNLKGVATNLYDYCIS
                     KEKADFDRFFHYLSVVLAFIAGAIVLVFCHHLFGAHSILIVSILFLIMALTQTFHLWQ
                     FYHTNRFTI"
     gene            complement(4600..5319)
                     /locus_tag="IV334_002336"
     CDS             complement(4600..5319)
                     /locus_tag="IV334_002336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HAP8944383.1"
                     /translation="MPIIVIIVAGTIGAGKSTLTEMLAQDLETKPFYENVEDNEVLPL
                     FYSNPEKYTFLLQIFFLNKRFLAIKDAFSHDDNVLDRSIYEDSMLFHLNADLGRVSEV
                     EVKQYEGLLETMLKELEEISPQKKPDLLVYIRVSFETMLARIKKRGREYEQLEQDPEL
                     YSYYKELNRRYEEWYEQFDICPKIVIDGDKYDFVADPACGEQIVQEIKMRAKKMTEEA
                     DAQSIRYTTNNPKAKGIFTNK"
     gene            complement(5631..5837)
                     /locus_tag="IV334_002337"
     CDS             complement(5631..5837)
                     /locus_tag="IV334_002337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8944384.1"
                     /translation="MENEQLFEVGETYAFKSDLFESPIKGKIEKIYENSVMIRVMKCA
                     VEDNDTAHNLHWQLVVRKSSTIEE"
     gene            6082..6684
                     /locus_tag="IV334_002338"
     CDS             6082..6684
                     /locus_tag="IV334_002338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory regulator AgrB"
                     /protein_id="HAP8944385.1"
                     /translation="MKDKVVNKLLEALFSGDKSEEDMVYVQVKFALEVLLNNLGKLAV
                     VLVFSLVTGSWAETGITFLSYICIRRYAYGLHSDSEFVCLLWTLLYLWGVPLVMKHLQ
                     LTISFPMMVFLLISCFLLLLRYGSRGTAINPIEPEKRPPLLKKAISMFLIFSLITLFF
                     SASYFSTYLLLGIVLEIATLLPITNYLMNFGGIFHEKNNH"
     gene            6825..8105
                     /locus_tag="IV334_002339"
     CDS             6825..8105
                     /locus_tag="IV334_002339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HAP8944386.1"
                     /translation="MFLWSIALQYLIYTICILLIDHHIRYKYLFAGVILISCILIGWV
                     YSILGIFTALIAIGMLMLTFFFFSTSRQQLVYALPFGLLTSLLGDHLTSIDDFFVLKS
                     SVIEPGDSLQYFHIVLSAFLSALLAFCLKKSLETWFHTVDRRMIGTIGTLILFTYYII
                     IFYTRFSGETPDVLALNTSFFILYLCVGLIILVYYWKISNKKLADQLLEQQVRMQQDY
                     IKDLEKNYQDLREFKHDYQNLLFSLHSYISEGDLEGLRSYYDQTILPTRQMMNLFPAN
                     LSMLDNMKVPEIRSLLSLKLMMAQEKGLIVQLHFPEPIELDTKHTVNLVRMLGIILDN
                     AIEGASAAKKKKIELTILKKNHSVMIRVINTTTNTLPLNQLKQKGFSTKTNPKNEGLG
                     LFILDELTKTNPYLFLETSIENQRFSQTIYIQIK"
     gene            8118..8846
                     /locus_tag="IV334_002340"
     CDS             8118..8846
                     /locus_tag="IV334_002340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP8944387.1"
                     /translation="MIPIYICEDDPKQLEVLSTVIKNRIMIENLDSYVHISTSDPEEL
                     LNAAKIRTSSFGIYFLDIELEDSEMDGVAIGKLIRELDPLGKIIYVTSHTEMSMKILK
                     SNIEPTDYIVKEDLYDLKENVEHILSKIFEDFQMSQIQKDIFKIEFNDEIKFLPIKDI
                     QYFSTSLGAPHKLEVHLTHAQLQFYEKIKEIEKMHRNFIRCHKSYVINTQNVRSINKK
                     TREVTLANGETIPASIRGLKKLIS"
BASE COUNT         2587 a         1667 c         1463 g         3188 t
ORIGIN      
        1 actcagttat cttatcatgt catttctaac atgtcaacaa ctattttata tgttttttaa
       61 aatactattt tattgctttg ctacttaaaa caacagaaat gaatatatca tgtaaattta
      121 cacatgtcaa tatattttta actctatatc cccacctaga tttgaatttt gaccacataa
      181 attgagcatg tctctatagt agaaaaataa agtcaaaaaa agaacacata acacgcttga
      241 tgctaagtgt tcgactgtta caactgtatg aaatgctcag tgacttgatt aaaacagtta
      301 aagtctattt atttaagata agctgttcca tcaactgctc attgtatcca ataaatagaa
      361 cttcatcgat aataatcaaa ggacgtctga ggagattggt atgttgtgtg cataaaaaaa
      421 tcagttgttt ttttcctaca caggaaaggt caatcgcaat gttgtgaaaa gcctcagacc
      481 aagtcgcaaa tagctgttct gcttgcactt cttcaaaact gcataattct tctgcttctt
      541 ctgtacttag atcgcgttct tctaagtcaa caaattgata aggcaattga tggtgttcta
      601 accattgttt cgccttcaga cttgttacac agtgcgctga tccatagact ttcacttcca
      661 cgaaggctcc ctccctatga atttaagatg ttattgtttt gtaaatagat cagatttctg
      721 gcaacacatc gaatttcacg agggacaaat gaccggatct catcttcatt aaatccgatt
      781 agtattcttt tttcatcaat caaaataggt cgtctcaata atgaaggata ttctttgaat
      841 aagacgatca tttgctttaa agttaattcg ttaaaatcta tgcccagctt ttcgtaaatt
      901 ttcgaccgag tggaaataat atcttgaact cctccttcag tcgctgccaa gatctttttg
      961 atctcacttt ctgttaacgg ttcggcaaaa atattcttct cttcaaattc aagttgattc
     1021 gcttgcaacc atgctcttgc ttttctacag gaagtacagc taggcgaagt atatactttt
     1081 atcattttct cactcatttt taattataat ccaattctac agtaatttct tttaaaatac
     1141 caattattag ctaataatat ataaaaaaaa atcaaaataa ctgattattt acgtttgaat
     1201 aatgagaatt tttctatata ttctctattt tgataatagt attctcattt tcaaaataaa
     1261 taagggaggt gtgacaaaat tcttgtcaca cctccctttt ccgaataaac tgtgttccag
     1321 aaatagcttc ttcgaaaata agctgaaatt tcacaaaaat ttgagaagca attttcgtac
     1381 attccttctt ctttcttgaa actgaccact tctgtcacac cctctcttct atcactttcc
     1441 ttttttcaca cgactcccgg tttctttgat aaatctcgat actttcaatt ttcttgaatt
     1501 tttcatttga ggtacacata gatacagacg atttcttgct ctagtcatcg caacataaaa
     1561 tagtcttcgt tcttcttcta tcgtcgtcac tggttctgct tttatttcat taaagtcgat
     1621 tactttcttt tctgtcgctc cgttataagg caaacttcct tcgttacaac caatcaaaaa
     1681 gacatttcgg taactaagac ctttactttt gtgaatgctc tggatacata gtgcatcaag
     1741 ttttccttgt ctgtaagcaa tcaagcgggt ttttaattct ttagctgtcg cttgggtttc
     1801 ttgcgataag gcttggagtc ttctgaaagt agcatgctgc tttacttctt ctcgtatcca
     1861 ttcttgatca ctctggtcga tttccttagc tatacgtggc agatgaagat agctttgaac
     1921 aagcgaatag atttcttgat gctggtctgt tttacgcaac gcttcctcaa actctcggat
     1981 ttctgcagat ttcgatttta aattggggct tatcgacaaa atataatctg caacggctat
     2041 attttgatat ttaggagaaa agtattgatc gcattcactt tttttcaggt agcagtctgc
     2101 catcagctgc cggagcaatt gctggtaggc ctgatttttc ttttgcagat tcagtgctgt
     2161 ttgccaatga acaatttttt gatagaaatg ttggaaagac ttgataccaa agataccttc
     2221 atatggacta tctgcttcca gtacaaaagg aatattcgtt ttcagcagag attggacaag
     2281 ctgctgcgtt tgtgaagctg atcggtataa tatgattgat tccttatatt tcccattttt
     2341 ttgaataaat gtctgaattt gtttgattag ccactgactt tctgctttag tatcagaaaa
     2401 caaataggct tccataacac ctgcttcttt ttgtgctgga atcatcgcct ttggtatccg
     2461 atcattttta ttatagctga tcaatctatt tgctccttgg acaatagctg cccgagaacg
     2521 ataattagtc aataagaaaa gagttttggt aggatattct ttttcaaagt tcttgatgta
     2581 tgctggctct gatcctctga atccatagat actctgatca tcatctccaa caacaatcaa
     2641 cttgatcctg ttttcctttg tcatccgttt cacgatttcc cattgaagtg gattagtatc
     2701 ttgaaattca tcaatgaata gataatcata tttttggccg atataatgct ggatctcctc
     2761 attctccaaa gcgcttttca gattttggag tacatcattg aactctagca attgctggct
     2821 tcttttttgt tcttggagtt ccgtgagcaa tccatacagt cgttctgata tcggctgtcg
     2881 tgcattctta tcaatggaat catatttttc tttaactaac tgaaccattt cttctactga
     2941 aaggttcata ttctgccaat agtctaaccg agaagcaatc gttttttctc catattgatc
     3001 aaatatataa agattatatt tacgtatcag ctgttgcaaa acagcattca ctttttgtgc
     3061 agtatccttt gaaaagtctc cttgtattcc ttgcattgct acttcaggaa attttcgaca
     3121 aatttcattg tatatcctaa aaaagatact atggaatgtt ccgatatgga cagcggtttt
     3181 tctctggctg aaattatcta ctcgtttttt tagttcattt gcagcgttcc ttgtaaaagt
     3241 gatcattagt atgcgttctt cgtctacttg tttttgcgtc atcagataaa cagcttttgc
     3301 acagattgtt tgcgttttgc cgctcccagc tcctgctatg attcgaagat gtttcttcat
     3361 atcatatgtg atagctgctt tttgttcttg attgaagcga ttataggaac gtttctccat
     3421 cattttaaag aaagagctgt ttccttcact ttcttttact tgtacttctt cctcaataag
     3481 tccgggtgct tcgccataag ccgcttcata ggtagcaatg atttcttctt gtagccgaaa
     3541 gacttgttga agctttttat acttgttctc ccagcgtttc aattcttttt gtaatcggag
     3601 aatttctttt tcattcaact gattgatttc cttcccatct ttcttctttg aatttgttta
     3661 tttcaagttg gttatttaaa gtttcgttgt ctattttttc tttatactaa cagacctttc
     3721 taaaaatgaa aatgggaagc gatatgattt tagttttctg aaaatttttc ctttttttca
     3781 atcgtttatg ctaagataag gaagacaaaa atattcataa aaggtgatga aaaaaatgca
     3841 gacttccttt cacgaggggc gtacaatagg acttttgctg acatttatcg gaggagccat
     3901 ggacagttat acttatatcc aatatggtgc ttttgcctct gctcaaactg gcaatatcat
     3961 tctatcgatc atccaagcat ttgacggaga atggctaagt gttggtaaaa aagtattgtc
     4021 tacccttttc tttttcttag ggattcttct aacaaagtat ctaattgatt attttagaaa
     4081 aaaagaaaaa catttctggc gcttgtttgt tctatattat gaagcagtga tttttttctt
     4141 tgttagtttg acacccgtcc atgagcatcc cgcattagta acgattttga ttgcttttac
     4201 tgcatcgatc cagtgggtag cttttgataa aatcaacggg cgtgcctaca ctaatttatt
     4261 tacaactgga aatttaaaag gggtagcaac gaacttatat gattattgta tttctaaaga
     4321 aaaagcagat tttgatcgat ttttccacta tctaagtgtc gttcttgcct ttatcgcagg
     4381 agctattgtc cttgttttct gtcatcatct gtttggcgcc cattcgattt tgatcgtttc
     4441 cattttattt ttgatcatgg ccctcactca gacatttcat ttatggcaat tttatcatac
     4501 gaatcggttc actatttgat ctcaatgaat caaaaaaact aagcaagatg ctgctcacag
     4561 agaaatatct gcgagtgagc aatttgctta gtttttatat tatttatttg taaagattcc
     4621 ctttgctttc gggttatttg tagtgtagcg gatcgactgg gcatctgcct cttctgtcat
     4681 tttctttgcc cgcatcttga tttcttgtac gatttgttct ccacatgccg gatcggctac
     4741 gaaatcatat ttgtccccat cgatcactat tttgggacat atatcaaact gttcatacca
     4801 ctcttcgtat cgtcgattga gttctttata gtaggaatag agttcaggat cttgttctag
     4861 ctgttcatat tcccggcctc tttttttgat tctcgctaac atagtttcaa aggatacacg
     4921 aatataaacc agtagatctg gtttcttttg tggcgaaatt tcttctaact ccttcagcat
     4981 cgtttctaaa agaccttcat actgctttac ttctacttca gaaacacgcc ctaaatctgc
     5041 attcaaatga aacagcatac tatcttcgta gatggagcgg tctagtacgt tgtcatcatg
     5101 gctgaaagca tctttgattg ccaaaaagcg tttgttcaaa aagaaaatct gtaataaaaa
     5161 agtatacttt tctggattag aataaaatag cggcaatact tcattatctt ccacgttctc
     5221 ataaaaaggt tttgtttcta aatcttgggc aagcatttca gttagtgtgc ttttccctgc
     5281 accgatcgtt cctgcaacaa tgatcacaat tatcggcatc cttcctgtaa taaaatctgt
     5341 tcttcatttt actacttcat ttatagaaat aaaagccttg actttatctg ctccttaaaa
     5401 gctacttctc taaaagtagc caaaagtaac tcctattcat gaaaataaag gctgtgacaa
     5461 aagtcttgtc gcagcccttt tttcaaataa acagtattct cctgccaaca cctcggtcac
     5521 aaggcaatat tcataaaaca tgaaaagctg ttttttaaca ttattccttg taactcagag
     5581 ctaaacggga gactcacaac ctctttatgc tctcaacatt ttatctgttt ttattcttct
     5641 attgtacttg atttgcgtac aactaactgc cagtgcagat tatgagcggt atcattgtct
     5701 tctactgcgc atttcatgac acgtatcatc acactatttt catagatttt ttctattttt
     5761 ccttttattg gtgattcaaa taaatcagat ttaaatgcat atgtttcgcc gacttcaaac
     5821 aattgttcat tttccataag aatccctcca tcacctttta taaggtcagt aattttttaa
     5881 cataaatcac tcgaaaaagc acaaacttgt cataaaaagt acttttcgta tcaaaactgt
     5941 cacttttttt ggtaaggttc ttggaaaacg aagatttttt taggagatca tgacggtttt
     6001 gatgtcaaaa ccacttttta tgaaacaaaa gacgtttttt atttttttta gcataaaatt
     6061 tgtggtggag gtacgtatga gatgaaagat aaagttgtga acaaattatt agaagcctta
     6121 ttcagcggag acaaatcgga agaagatatg gtgtatgtcc aagtaaaatt tgccttagaa
     6181 gttttattga ataatcttgg aaaactagcg gtcgttctag tcttttctct ggtgacaggc
     6241 tcatgggcgg aaacaggaat cacctttctt tcttatatct gcatccgcag atatgcttat
     6301 ggattgcatt ccgactctga attcgtttgt ctgctgtgga cactacttta tttatggggc
     6361 gttcccttgg tgatgaaaca tctgcaattg actatctcct tcccaatgat ggtctttttg
     6421 ttaatcagct gctttttgtt actgcttcgc tatggatcaa gaggtacagc aatcaatccc
     6481 attgaacccg agaaacgtcc cccattatta aagaaggcga tcagcatgtt tctaattttt
     6541 tctctcatta ctttattttt ctcagcttct tacttttcta cctatttgct acttgggatt
     6601 gttttagaaa tagctacgtt gcttcctatt acaaactact tgatgaattt tggaggaatc
     6661 tttcatgaaa aaaacaatca ttaaacaatt aactaactta tctgactttt ttgtatctgg
     6721 ctgctgcttt ggtatgagtt ctgaattcaa aatccctgaa aaattaaaaa aataatacgc
     6781 taattgtgct ataatttggt aaaaatcaat gggagctgta ctttatgttt ttatggtcca
     6841 tagcgttgca atatttgatt tacacaattt gtatcttatt gattgaccat cacatccgat
     6901 acaaatatct gtttgcggga gtcattctga tttcttgtat tttgattgga tgggtctatt
     6961 cgattttagg gatttttact gctctaattg ctataggaat gctgatgctt acttttttct
     7021 tcttttcgac ttcgcgtcaa caattggttt acgcgcttcc ttttgggtta ttgacgtcgc
     7081 ttttaggaga tcacttaaca agcatcgacg atttttttgt tttgaaatct tcggttattg
     7141 aaccaggaga ttcattgcag tattttcaca ttgtcttatc cgctttttta tctgctttat
     7201 tagctttttg tttaaaaaaa tcgttagaga cttggtttca cactgtggac cgccgtatga
     7261 tcggtacgat cgggacattg atattattta catactatat cattattttt tatacccgtt
     7321 tttctggtga aacacctgat gtcttagctt tgaatacgag ttttttcatt ctatatctgt
     7381 gtgttggcct gatcatcttg gtttattatt ggaaaatctc taataagaaa ttagctgatc
     7441 agttattaga gcaacaagtc aggatgcaac aggattatat caaagatcta gaaaaaaact
     7501 atcaagactt gagagaattc aaacatgatt accaaaatct gcttttttct cttcacagct
     7561 atatttcaga aggcgactta gaaggattac ggtcttatta tgatcaaacg atattaccta
     7621 cacgccagat gatgaacctt tttccggcca atctaagtat gttggataat atgaaagttc
     7681 ctgagatccg tagtttactg tcattaaaac tcatgatggc acaggaaaaa gggttgatcg
     7741 tccagcttca ttttcctgag ccaatcgaac tggatacgaa acatacggta aaccttgtac
     7801 gtatgcttgg tattatcttg gacaatgcga ttgaaggagc tagtgctgca aagaagaaaa
     7861 aaatcgaatt gaccatactc aagaagaatc attccgtgat gattcgtgtg atcaatacaa
     7921 caacaaatac attaccgtta aaccagttga aacaaaaagg gttctcaaca aaaacaaatc
     7981 caaaaaacga aggccttggc ttgtttatct tggatgaatt gacaaagacc aatccttatc
     8041 ttttccttga aacatcaatt gaaaaccagc gtttttctca aacgatttat atacaaataa
     8101 aataaaggag taatgatatg attcccattt atatttgtga agacgatcca aaacaattag
     8161 aagttttatc aacggtgatc aaaaatagaa tcatgattga aaacttagac agttatgtcc
     8221 atatttctac ctctgatccg gaagaacttc tcaatgcagc caagatcaga acttcttctt
     8281 ttggtattta ctttttagat atcgaactag aagacagtga aatggatggt gtagcgatcg
     8341 gtaaattgat ccgtgaatta gatcctttag gaaaaataat ctatgtgact tcccatacgg
     8401 aaatgtcgat gaaaatctta aaaagcaaca tcgaaccgac tgattatatc gtcaaagaag
     8461 atctttatga tttaaaagaa aatgtcgagc atatcctgag caaaattttc gaagattttc
     8521 aaatgtccca aatccaaaaa gatatcttca aaatagagtt taatgatgag atcaaatttt
     8581 taccgatcaa agacatccaa tatttttcta cttcgctagg agcgccacat aaactggaag
     8641 tccatttgac tcatgcccaa ttacaattct atgagaagat caaagaaatc gagaaaatgc
     8701 atcggaattt tatccgttgt cataaatcct atgttatcaa tacacaaaat gtgcggtcga
     8761 ttaataaaaa aaccagagaa gtcacgttgg caaacggcga gaccatccca gcctctatcc
     8821 gtggattaaa gaaattgatt tcataaaaag aaccgaatag tttggaccga ctaaatgacc
     8881 agccgatcca aacccttcgg ttctt
//
LOCUS       DACDKM010000108         8494 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20834_2016
            SAMN12640370-rid9891663.denovo.108, whole genome shotgun sequence.
ACCESSION   DACDKM010000108 DACDKM010000000
VERSION     DACDKM010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640370
            Sequence Read Archive: SRR10040891
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8494)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8494)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742835.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742835.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:04:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,012
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 29 of 75
            Pseudo Genes (incomplete)         :: 33 of 75
            Pseudo Genes (internal stop)      :: 19 of 75
            Pseudo Genes (multiple problems)  :: 5 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8494
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640370-rid9891663.denovo.108"
                     /isolate="E20834_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(<1..173)
                     /locus_tag="IV334_002341"
     CDS             complement(<1..173)
                     /locus_tag="IV334_002341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009325336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP8944388.1"
                     /translation="MATKIADELENHVLETGMQKITTHASITAKPFFEKRGYKVINEQ
                     TVELRGQLFTNFL"
     gene            complement(593..1714)
                     /locus_tag="IV334_002342"
     CDS             complement(593..1714)
                     /locus_tag="IV334_002342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010730282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
                     /protein_id="HAP8944389.1"
                     /translation="MDKYNVKTSFQKWFSAINFSGLSENSQSLISDFDFYSKKLDFQT
                     TLKVLLHAVYEELPSYREIDRAFLDQRLCKELGIDSLCYSSLSRRAPEIKQEVLMEIF
                     TQLVARISAQQPSSKTTSLQLIDSTTIPLNKAWFPWAKFRKTKSGIKLHLNLCYLDKT
                     NQYPESFTMTNASEHDRNHLEVLVDKTQATYVVDRGYFDYKLLDKLNRDGYFFVTRTK
                     SNTKITILDQIEVADTTTRDGTIISDQQVILVGGVNHVTERFRLVTVLTKGQKILRMV
                     TNLFDVSPNEVADMYQARWQIELLFKHLKQNLTIKRLYSHSEQGAINQVILTLIATLL
                     TYVIKIELNTTATLFQLKRTFHYLMFEPAEEWFKRYRPG"
     gene            2042..3451
                     /locus_tag="IV334_002343"
     CDS             2042..3451
                     /locus_tag="IV334_002343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS21 family transposase"
                     /protein_id="HAP8944390.1"
                     /translation="MDDIKTIKHLRNNRDQSIDQIRKAMNINWRTAKKYADSDQLFIT
                     DPTRKNKSMMDDGWGEIIGSWLEEDLRLRRKLRRTAKAIHEQLKPLGFPGSYRTVCVY
                     ISQWKKQRYDETNYQAVDRLDHPIGEAQVDFGTMEVMHEDHMKDIQLLIMSFPYSNAT
                     SASAMPAQNQECFLEALKQLFNQCGGVPKTIRIDNLTPAVKKTKSKFEEAQLTDEFLR
                     FTIHYGCDVQVCNPRSGHEKGHVENKVGYVRYNFFSTSPIMDSFESLNKQLEEQLKAD
                     RKRTHYSKHQLIEDLWKKENKALHSLPQEDYPIFKEVEAKLNRYHEMKLDQTVIHIPK
                     AYGYSQLTVVLTWNKFKVLTPHGEILMNEYRPYMNKKRLIPWSDIMKQWSNKLNVVSY
                     SRYWKYLPARLQNYLAIDNNHLKYRRIKQLLSLLATYDIQQINEQFYELVVQEDAPSD
                     FDVNWSNYDALTKTGGVQK"
     gene            3448..4176
                     /locus_tag="IV334_002344"
     CDS             3448..4176
                     /locus_tag="IV334_002344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HAP8944391.1"
                     /translation="MIGQTLEDYCKKLRLSHIITNIPRASFEAPEQFITDLFRLELET
                     RQQIKLERMEKLAKFPVPKSLKNYEWHEDIFLPSHLDKETLVSLDFLKRQENIVCVGS
                     PGTGKTHLAIALGRKACSEGVSTKFFRVNNLVEQLENALKKQQLASFKKRFETTELII
                     LDEMGYLPLTKEGAELLFQLISDWYEQKSIIITSNLEFSQWNRVFMDSRLTAALVDRL
                     IHHAHIISFKGESFRLKNSLSKSY"
     gene            complement(4304..5215)
                     /locus_tag="IV334_002345"
     CDS             complement(4304..5215)
                     /locus_tag="IV334_002345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAP8944392.1"
                     /translation="MKIKIERSNKEKIPKKKKERVVSVGKHRKMVLALWLLLSCSLAF
                     GIYKNFTAIDQHTTHEKVVVKEKIVNTSGVENFTVDFVKEYFSWKNNKEVIEKRMTNL
                     EQYLTEEGLALSQDMVRADIPTSSEVVSVKILDVEKHSEEFVVSFVVDQKITEGKKTQ
                     SISSAYRLMIFEDEKGNHIVTSLPTMIGKPEKAKHKVRQVKSDSKIDSKTTEEITEFL
                     ETFFRLYPAASEKELEHYVEKDAMQPINTNLKFVELVNPIYKQKGKAIQVIVGVEYLD
                     ELSKTTSVFQYRLKLYKNENWTISNCE"
     gene            complement(5232..6239)
                     /locus_tag="IV334_002346"
     CDS             complement(5232..6239)
                     /locus_tag="IV334_002346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HAP8944393.1"
                     /translation="MNLKKLSIISLTFLTISFVGILMCVGLLFGEDGEGSGSSGTSPG
                     GSSVSEVVLKHRSMVEKYAKESDITEYVSILLAIIQVESGGTMEDVMQSSESAGLSPN
                     SLSTEASIKQVCLYFSSLVKCSKELGCDQDSIIQAYNYGDGYLDYVAKNGKKHSFALA
                     ESFSKEKSGGQKVDYPNPIAIKENSGWRYNYGNMFYCLLVKQYLTTTQFDDKTVQGIF
                     DEAFKYEGTAYVFGGSSPGTGFDCSGLTQWCYAKVGLKLPRVAQDQYDAMTHIDLKDA
                     KPGDLIFFHSTYDAGTYVTHVGIYAGENRMFHAGDPVGWTNLTDSYWQQHIIGAGRYK
                     Q"
     gene            complement(6236..8365)
                     /locus_tag="IV334_002347"
     CDS             complement(6236..8365)
                     /locus_tag="IV334_002347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HAP8944394.1"
                     /translation="MKKKLFRIVGIVFISIVTLTVLLSLVGTVAEATGLVDDTVEAGN
                     LYSKYSLNNYQLDFFVDSSWDWLPWNWGDGLGKSVMYGLYAITNFIWTVSLYLSNATG
                     YVVQEAYKLDFISDTAKSIGKNIQTLAGITENGLQTSGFYFGFLLLMILALGVYVAYT
                     GLLKRETTKAVRTVINFVMIFLLSGSFIAYAPTYITKINDFSSDVSEAALSLGTKIVV
                     PNSESQGKDSVDLIRDSLFSIQVQQPWLLLQFDDSNIEEIGEGRVNKILSVSPDENKG
                     KDREEAVKAEIEDNDNANLSITKTMNRLGTVVFLVLFNIGISFFVFLLTGIMLFSQIL
                     FIIFAMFLPISFLLSMLPTYESLGKRAIIRLFNTIMMRAGVTLVITTAFSISTMFFNI
                     SATYPFFMVAFLQIVTFAGIYFKLGDIMSMFNLQSNDSQSMGRRVMRKPQMLMNRKLR
                     QLNRNVGRTFAFGGGAAVGGKLATSSTKGKGSSKINRVSNRKNHSARSINELNSDLKK
                     QEQPVGTKKESRINLVGRKVGKVLDTKEIVKDKAKQAKNQVTDAPTNLKYSLHKGMEK
                     TKKVPEDFKRGPFEEKANRGELREKQRQRRDAKMDEKRKVLNEAWNRHEKGRENVPIK
                     KEIKPQKNELPENRSLKPRVQVHSNPEIKRKLSSQEVIPNGSTQPLSKNNSFQQVKQR
                     FTFPKRTNQQVQRKKNTRPKPKPGEKR"
     gene            complement(8370..8477)
                     /locus_tag="IV334_002348"
     CDS             complement(8370..8477)
                     /locus_tag="IV334_002348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8944395.1"
                     /translation="MQDLYGRVGVVQVHPVFEELFHAFDTRPPVQERME"
BASE COUNT         2599 a         1728 c         1486 g         2681 t
ORIGIN      
        1 attaaaaagt tagtaaataa ttgccctcta agttccacgg tctgttcatt aataacttta
       61 tatcctcgtt tttcaaaaaa tggctttgcg gtaatggaag cgtgggtagt gattttttgc
      121 ataccagttt ctaaaacatg gttttccaat tcatctgcaa ttttagtcgc aatacctttt
      181 ccttgatggc tatgtttttg tcacaacttc agtattcctg taacaatacg gatttcagta
      241 tacaacaaca gcttttgaaa agatacttgc aaaaggatta agatatctat gacctgctat
      301 gctcactgat gttgaaaaga atgttttatt aggattgagg taagcaagat agtgaatatg
      361 tccggaaagc ctgtaaaata aggcttttcg gacatatgtg aagttataaa aagatagcga
      421 aaaatctatt tagtatcact aatgttgcga acatatcgtc agaatagtct gacatttatt
      481 tcttgctaaa aatttcaaaa aagtcaagtg ctgacgcagc ggatgactat tttccagaaa
      541 attttagtat gtattcaaaa atcaaacgtg acaacagcta ttaactacat cctcatccag
      601 gtctgtaacg tttgaaccac tcttctgcgg gctcaaacat taagtaatgg aaggtacgtt
      661 tcaattgaaa cagcgtcgct gtcgtattca actcaatttt tatcacataa gtgagaagcg
      721 ttgcgattaa ggtgaggatg acttgattta tcgccccttg ttcactatgt gaatatagtc
      781 gtttaatggt cagattttgt tttaagtgtt tgaacaacaa ttcaatctgc caacgggctt
      841 gatacatgtc ggccacttcg tttggcgata catcaaagag attggtcacc attcgcagga
      901 ttttttgtcc cttggtaaga accgttacca acctaaaacg ctctgtcaca tggttgacac
      961 cgccgacaag aatcacttgt tggtcactga ttatcgtccc atcacgggtt gttgtatctg
     1021 ctacttcaat ttgatccaag atggtaattt ttgtattaga ttttgtccgt gtaacgaaga
     1081 aatagccgtc acgatttagt ttgtctaaca acttgtaatc aaagtagccg cgatccacca
     1141 catacgttgc ttgcgtttta tcaacaagaa cttcaaggtg attacggtca tgctcactcg
     1201 catttgtcat cgtaaaactt tcaggatact gattggtttt atcaagataa cagagattca
     1261 aatgtagctt gatgccagac tttgttttcc gaaacttggc ccatggaaac catgctttgt
     1321 tcaacggaat agtcgttgaa tcaatcaatt gtagactggt tgtttttgag cttggctgct
     1381 gagcagaaat tctggcgact aattgcgtaa aaatttccat caatacttct tgcttgattt
     1441 ctggcgctct acgagataac gagctgtaac acagggaatc aatgccaagt tccttgcaga
     1501 ggcgctgatc taaaaaggcg cgatcaatct cgcgatagct tggcaattct tcataaaccg
     1561 catgaagtaa cactttaagc gtcgtttgga agtctagttt tttactgtag aaatcaaagt
     1621 cgctaattaa gctttgagag ttttctgata aaccagaaaa atttattgca gaaaaccatt
     1681 tttgaaatga ggtttttacg ttatacttat ccatgcgtga gtccttttta ttggtcttgg
     1741 ataagtcatc cgagaatagt ataaaggact tttttgcgtt tggatagctt tgtattaggc
     1801 ttttgatggg ttaaaaagtc tttgcaacac tagtaattta gtatttatgt ataaattgaa
     1861 agatatttta ccaccactta gtcttcttaa aagtgatcca acattttgtt tgtggcagaa
     1921 tttaactaca cactaatgca gccgaaagta cacaactatg cagcagtttg actgcgaact
     1981 ggtttccttc catcataagt ttgaatgact tagtgatgga ggtacagaaa ggttgttaac
     2041 tttggatgat attaaaacaa taaaacattt gagaaataat cgtgatcagt caattgatca
     2101 gataagaaaa gcaatgaata tcaattggcg aacagcgaaa aaatacgccg atagtgatca
     2161 actattcatt acagatccca ctcgaaaaaa caaatccatg atggatgatg gttggggaga
     2221 aatcatcggt agttggctag aagaagacct acgtttacgc agaaaacttc gtagaacggc
     2281 caaagctatc catgaacaat tgaaaccttt gggtttccct ggctcctatc gaacagtttg
     2341 tgtatacatt tctcaatgga aaaagcagcg ttacgacgaa acgaattacc aagctgtcga
     2401 tcgtcttgat catccgatcg gagaagcaca ggtcgatttt ggaacaatgg aagttatgca
     2461 tgaagatcac atgaaagata tacaacttct tatcatgtca tttccctata gtaatgcgac
     2521 gtccgcaagt gccatgccag ctcaaaacca agagtgtttc ctggaagcat taaaacaact
     2581 atttaatcaa tgcggcggcg ttccaaaaac gatcagaata gataacttaa ctccagcggt
     2641 gaaaaaaaca aaatcaaaat ttgaagaagc acaactaacc gatgaattct tacgtttcac
     2701 cattcattat ggttgtgatg ttcaagtctg taacccacgt agtgggcatg aaaaaggaca
     2761 tgtcgaaaat aaagtgggtt atgtccgata caatttcttc tcgacttcgc caatcatgga
     2821 ctcttttgag tccctaaata agcaacttga agaacaatta aaagcagatc gaaaaaggac
     2881 gcattattca aaacatcaat taatcgaaga tttgtggaag aaagaaaata aagcgttaca
     2941 ttctcttcca caagaggatt acccaatttt taaagaagtg gaagctaaac taaatcgtta
     3001 tcacgaaatg aaattggatc aaacggttat tcatattcct aaagcatatg gctattctca
     3061 attgactgtt gttttgactt ggaataaatt caaagttctt acgcctcatg gagaaatatt
     3121 aatgaacgaa tatcgcccct atatgaacaa aaaaagattg atcccatgga gtgacatcat
     3181 gaaacaatgg agtaataaat tgaatgtcgt tagttattcg aggtattgga agtacttgcc
     3241 tgcacgcctt caaaattatc tagcgattga taataatcac ctgaaataca gacggataaa
     3301 acaattacta agtttattag cgacctacga tatccaacaa attaatgaac aattttacga
     3361 attagtcgtt caagaagacg ccccctctga ctttgatgtg aattggtcaa actatgatgc
     3421 attaacaaaa acaggaggtg tgcaaaaatg attggacaga cgttagaaga ttattgtaaa
     3481 aagcttcgat taagccatat catcacgaac ataccaagag cttctttcga agctccagaa
     3541 caatttatta ccgatttatt ccgtttagaa ctggagacca gacagcagat caaattagaa
     3601 cggatggaaa aattagccaa atttcctgta ccaaaatcgc taaagaacta tgaatggcac
     3661 gaagatattt ttctaccatc tcatctagat aaggagaccc ttgtctctct tgattttcta
     3721 aaaaggcaag aaaacattgt ttgtgttggc tctcctggta cagggaaaac acatctagcg
     3781 atcgcattag ggagaaaagc ctgtagtgaa ggtgtttcaa ccaaattttt ccgagttaac
     3841 aatctcgtag agcaattaga gaatgcatta aaaaaacaac agctagccag tttcaagaag
     3901 cgattcgaaa caacagaatt aattattctt gatgaaatgg ggtacttacc attgacaaaa
     3961 gaaggagctg aacttctctt ccaactgatt tcagattggt atgagcaaaa gagcattatt
     4021 attacctcta atttagaatt cagtcaatgg aatcgagtgt ttatggattc tcgattaaca
     4081 gctgctctcg ttgatcggct aatccatcac gctcacatta tcagcttcaa aggcgaaagt
     4141 ttcagattga aaaattcatt atctaaatca tattgaagga aaaaactagt tgcagcaaaa
     4201 ctctgcactt tcggctgcat ttttttgtaa aaagctattg cacaacacaa acatttgaac
     4261 gacgtaattc caaatgttgg attgctttta gataatcttt tatttattca caattgctaa
     4321 tagtccaatt ttcatttttg tatagtttta atctatactg aaaaacactt gttgttttgg
     4381 acaactcatc aagatactca acaccaacaa tcacttgaat cgctttaccc ttttgtttgt
     4441 aaataggatt cactaattcc acaaatttca aattagtatt aattggttgc atcgcgtctt
     4501 tttcaacata atgctctagt tctttttctg aggctgctgg ataaagcctg aaaaacgtct
     4561 ctaaaaactc ggtgatttct tccgttgtct ttgaatcaat ttttgaatca ctttttactt
     4621 gtcttacttt atgttttgcc ttttctggct taccaatcat agtgggtaaa ctcgttacaa
     4681 tatgattgcc tttttcatct tcaaagatca tcagacgata tgcagaagaa attgattgtg
     4741 tctttttccc ttctgtaatc ttttgatcta ccacaaatga aacgacaaat tcttccgagt
     4801 gtttttcaac atctagaatt tttactgata caacctcgga actagtggga atatccgctc
     4861 gaaccatatc ttggctcaaa gcgagccctt cttctgtaag gtactgctca agattagtca
     4921 ttctcttttc aattacctcc ttgttattct tccatgaaaa atattctttc acaaaatcta
     4981 cagtaaaatt ttctactcca gaggtattca ctattttctc tttcacgacc actttctcat
     5041 gagttgtatg ctgatcaatg gccgtgaagt ttttgtagat tccaaaagca aggctacaag
     5101 acaataaaag ccaaagggca agcaccattt ttcgatgctt gcccacggat actactcgtt
     5161 ctttcttctt ttttggaatc ttttccttgt tacttcgctc aatttttatc ttcattttcc
     5221 attcctcttt tctattgttt ataccgacca gcaccaataa tgtgctgttg ccaatagcta
     5281 tcggtaagat tggtccaacc caccggatct cctgcatgaa acattctgtt ctctcctgca
     5341 taaatgccca catgagtcac ataagtaccg gcatcataag ttgaatgaaa gaaaatcaaa
     5401 tcgcctggtt tggcatcttt taagtcaata tgagtcatag catcatattg atcctgagcc
     5461 acccgaggga gtttcaaacc aactttggca taacaccatt gagttaaacc actacaatca
     5521 aacccagttc cgggacttga cccaccaaat acataagccg ttccttcata cttgaacgcc
     5581 tcgtcaaaaa ttccttggac tgttttatca tcaaactgtg ttgtagttag gtactgcttc
     5641 actaataagc aataaaacat attgccatag ttataccgcc acccactatt ttccttaatc
     5701 gcgatgggat tgggataatc gactttttga ccacccgatt tttcttttga aaaagattcg
     5761 gctaaagcaa aactatgctt ctttccgttt ttcgcgacat aatccaaata cccgtcacca
     5821 tagttataag cctgaatgat actatcttga tcgcatccta gttctttgga acacttaacc
     5881 aaagacgaaa aatagagaca cacttgtttg atggatgcct cggtacttaa tgaattcggt
     5941 gataaacctg ctgattcaga actttgcatg acatcttcca ttgtacctcc agattcgact
     6001 tgaataatcg ccaaaagaat cgaaacatac tctgttatat ctgattcttt ggcatacttt
     6061 tccaccattg aacggtgttt caaaactact tctgagacac tactacctcc aggagaagtt
     6121 ccagaactac cacttccctc accgtcctca ccaaacaaca aacccacaca catcaagatg
     6181 cccacaaaag agatggtcag gaaggttaac gaaataatac tcaatttttt taagttcatc
     6241 ttttctcacc tggctttggt tttgggcgag tgttcttttt tctttgtact tgttgattgg
     6301 ttcgctttgg aaaagtgaat ctctgtttca cttgttgaaa ggaattgttc ttgcttagtg
     6361 gctgagtgct tccgtttgga atgacttctt gactagaaag tttccgtttg atttcaggat
     6421 tcgaatgtac ttgtaccctt ggttttaaag acctattttc tggtagttca tttttctgtg
     6481 gttttatctc cttttttatc ggaacatttt cccttccttt ctcatgacga ttccatgctt
     6541 cgttcaatac tttgcgtttt tcatccattt ttgcgtctct tcgttgtcgt tgtttttctc
     6601 gcaactcccc tcgatttgct ttttcttcga aaggccctcg cttaaagtcc tctggtactt
     6661 ttttcgtctt ttccatgcct ttatgaaggc tgtatttcaa gttagttggt gcatcggtta
     6721 cttgattttt tgcttgtttc gctttgtcct tcacgatttc ttttgtatcg agtacttttc
     6781 caacttttct accaactaaa tttattcgtg attctttctt cgtgcccact ggttgttctt
     6841 gttttttcag atcagaattt agctcgttaa tgcttctcgc ggaatggttc tttctattgg
     6901 acactctatt gatttttgaa ctacctttcc cttttgtaga actggtagct agtttcccac
     6961 caactgctgc accgccacca aaagcaaaag ttcgacccac atttcgatta agctgtcgca
     7021 attttcgatt catcaacatt tgcggtttcc gcatgactcg ccttcccatt gactgactat
     7081 cattactttg aaggttaaac atgctcataa tgtcacctaa tttgaaatag ataccggcaa
     7141 aggtcacaat ttgtagaaaa gctaccataa agaagggata ggtggccgaa atattgaaaa
     7201 acatcgtgga gatactgaat gcagtcgtaa tgactaaggt aactcccgct cgcatcataa
     7261 tcgtattgaa caaacgaata atcgcccttt tgcccaaact ctcatacgtc ggcaacatcg
     7321 acaataagaa actaatgggc agaaacatcg caaagataat gaaaaggatt tgtgagaaca
     7381 acataatccc tgtcagaaga aacacaaaaa aggaaatccc aatattaaac aacactaaaa
     7441 agaccacggt tcctaaacgg ttcatggtct tggtaatact gagattcgca ttgtcattat
     7501 cttcaatctc tgctttgact gcttcttccc gatccttacc cttattttca tccggactca
     7561 cggataaaat cttattaacc cgaccctcgc caatttcttc tatattggaa tcatcgaatt
     7621 gtaatagtaa ccagggttgt tgaacttgaa tcgaaaataa actgtctcga atcaaatcca
     7681 cactatcttt tccctggctt tctgaattag gtacaacaat cttggttcca aggctaagag
     7741 ccgcttcact aacgtctgag ctaaagtcat tgatttttgt aatataggtg ggcgcatacg
     7801 caatgaaaga acccgagagt agaaaaatca tcacaaaatt aatgacggtt cggactgctt
     7861 tggtagtttc tcgttttaat aatccggtat acgccacata aactcctaga gctaaaatca
     7921 tcaataatag gaacccaaag tagaatcccg aagtctgtaa tccattttca gtgataccgg
     7981 ctaaagtttg gatattcttt ccaatacttt tagccgtatc cgaaatgaaa tccaacttgt
     8041 aagcttcttg aaccacatag cccgtggcgt ttgataaata cagacttact gtccaaataa
     8101 aattggtaat ggcataaaga ccatacatca cgcttttccc taagccatcc ccccaattcc
     8161 aaggtaacca atcccaagaa ctatccacaa agaaatccag ttgataattg ttcagagaat
     8221 atttggaata aaggtttccg gcttcaactg tatcatcaac cagacctgtc gcctcagcta
     8281 ccgtaccgac taaactcaat agaactgtca gagtgacgat gctaataaaa acgattccca
     8341 caatccgaaa aagtttcttt ttcatcccct cactccattc tctcttgaac cggcggacga
     8401 gtgtcaaaag catggaagag ttcttcaaag actggatgaa cttgaaccac acctactcgc
     8461 ccatacaagt cttgcatcaa gcattgaccg tttt
//
LOCUS       DACDKM010000109         8404 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20834_2016
            SAMN12640370-rid9891663.denovo.109, whole genome shotgun sequence.
ACCESSION   DACDKM010000109 DACDKM010000000
VERSION     DACDKM010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640370
            Sequence Read Archive: SRR10040891
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8404)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8404)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742835.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742835.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:04:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,012
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 29 of 75
            Pseudo Genes (incomplete)         :: 33 of 75
            Pseudo Genes (internal stop)      :: 19 of 75
            Pseudo Genes (multiple problems)  :: 5 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8404
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640370-rid9891663.denovo.109"
                     /isolate="E20834_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(158..616)
                     /locus_tag="IV334_002349"
     CDS             complement(158..616)
                     /locus_tag="IV334_002349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HAP8944396.1"
                     /translation="MHFFKTRNILKAVASGQIIPLTAVKDEVFSSKMIGVGYAITNHD
                     GYIHAPVKGIIDNIFPTRHAITIESESGRKILIHMGLDTVELEGSPFQIRVESNQEVK
                     EGTLLAIIDLKKLEQAQKDTVIIVVSPGIFEGNLLKQNQEVKIKDSCFEF"
     gene            complement(629..1954)
                     /locus_tag="IV334_002350"
     CDS             complement(629..1954)
                     /locus_tag="IV334_002350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005227470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose-6'-phosphate glucosidase"
                     /protein_id="HAP8944397.1"
                     /translation="MKKQIITIAGGGSTYTPGIVQAILNNEKRLPLSEIRLYDIDEER
                     NMDMYLIVKFMLKRKGFSNIRIRATDDPKLAFTGCDFVFSQIRVGGLEMREKDEKIPL
                     KHGLVGQETCGLGGFAYGMRSIKGLLEIVDHIQDYAPKAWILNYTNPESIVSEAVRRK
                     YPNVKMINACDMTISIEETIAVNYGYDRKNWIVTYYGLNHFGWYTSIYDKELQREIMP
                     EIIEKLITKEMQVADFNIGDKTWQKTFQMMSVITKNFPSNIPNNYLEYYLYPDMVVEH
                     TDKEYTRANMVMDGREKNTKEMADKIRRGIVEEVLNFNFGEHGQYIVDIAISLLNDDR
                     RRFMLIVPNQGAIPNLRSDAVVEIPAYVGATGVEPITLRKPISDFHKGLMEAQVAVEK
                     LLVDAYFEGSYQKALQAFTLNQTVPNARVAKKILDEMIKVNKKYWPALK"
     gene            complement(1971..3383)
                     /locus_tag="IV334_002351"
     CDS             complement(1971..3383)
                     /locus_tag="IV334_002351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP8944398.1"
                     /translation="MNVKSNVQKLGRAMLLPVAAMPLAGLIMRLSADDMLNIPVIGAA
                     GNAVFGNLDILFAIGVAIGFVKNKDKGIPALTGFLAIATLKKGLEIMDPEVNMGIFGG
                     IISGLVAAWTYNRFKEQKLPVVFSYFAGEKFPLTMVMILQTIAAVFLGFIWPLIQIGI
                     DNFAKLLVDMGALGVGIFMFLNRLLIPFGLHHVLNTYIYYDLGSYTSPSKEVFRGEMT
                     RFINGDPQAGLFLSVFFVVMMFGIPAICLAIYKAAYKENKEMVKGIMGSGAGTSFIAN
                     ITEPVEFSFMFLSPMLYVIHALFAGLAGIVCYLFNIRIGFTFGACIVDYLINFRIATN
                     AILILPIGIFFFALYYVTFYYLINKRNIQTLGREAKAEFGNEVTLEETELGLASKNYE
                     YMATKMLQAFGGKANILDVYSCNTRLRVEVVDPTMVEEQRIKQLGISGIIKPTEKNYQ
                     IIIGLEVTYVMAEFNKLLEE"
     gene            complement(3400..4164)
                     /locus_tag="IV334_002352"
     CDS             complement(3400..4164)
                     /locus_tag="IV334_002352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate deacetylase"
                     /protein_id="HAP8944399.1"
                     /translation="MGKVIFNSDDFGYSHGVNYGIMDAYQRGILTSTTLMANMPGFEH
                     AVKLRKEMPRLGVGVHLTLTCGKPLLKTVDTLIESGEFRSLSYYQHPFNIDKDQLYQE
                     WNAQIQKIYHAGIIPTHLDSHHHTHTLDENQEVVVTLAKKYDLPVRSNFERKDEVRHV
                     DYFEPCFDVVGETDEKKRQINTSVEEYLASLLMILREDKTIEVMCHAAYLDQELLNGS
                     SFVYPRTHQVEFLIHSAFAQKVKNDPLIELVTYEDI"
     gene            complement(4268..5143)
                     /locus_tag="IV334_002353"
     CDS             complement(4268..5143)
                     /locus_tag="IV334_002353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAP8944400.1"
                     /translation="MKESASCFVLSYRGGELTVYVMKKLNHNAVLIDDDGIEKIAMGK
                     GIGFSAKVSKPFDVEDADKLFVLDSKEKIKRFSEIVSQIPIEFMDFAEEVIELITARI
                     SSPLDSNIYITLTDHIYFAIQRKREDRDVTAIMLPEMRFLYPNEYDIAVEVVERINHR
                     YQTELGSNEVGFITMHIINAELGEPNSLKSLKIVEMTTTILSHLEKSYSNVYDKESLT
                     YHRLMVHIKFLVKRLIYQEETPVENIGFFTETFKHSKAYCTALKIKDIIEQQFQLAIQ
                     ENEIIYLAIHLARIY"
     gene            complement(5398..5482)
                     /locus_tag="IV334_002354"
     tRNA            complement(5398..5482)
                     /locus_tag="IV334_002354"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5446..5448),aa:Leu,seq:caa)
     gene            complement(5500..5571)
                     /locus_tag="IV334_002355"
     tRNA            complement(5500..5571)
                     /locus_tag="IV334_002355"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5537..5539),aa:Gln,seq:ttg)
     gene            complement(5581..5653)
                     /locus_tag="IV334_002356"
     tRNA            complement(5581..5653)
                     /locus_tag="IV334_002356"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5618..5620),aa:His,seq:gtg)
     gene            complement(5715..5799)
                     /locus_tag="IV334_002357"
     tRNA            complement(5715..5799)
                     /locus_tag="IV334_002357"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5763..5765),aa:Leu,seq:caa)
     gene            complement(5820..5890)
                     /locus_tag="IV334_002358"
     tRNA            complement(5820..5890)
                     /locus_tag="IV334_002358"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5856..5858),aa:Cys,seq:gca)
     gene            complement(5936..6007)
                     /locus_tag="IV334_002359"
     tRNA            complement(5936..6007)
                     /locus_tag="IV334_002359"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5973..5975),aa:Gln,seq:ttg)
     gene            complement(6019..6091)
                     /locus_tag="IV334_002360"
     tRNA            complement(6019..6091)
                     /locus_tag="IV334_002360"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6056..6058),aa:His,seq:gtg)
     gene            complement(6094..6167)
                     /locus_tag="IV334_002361"
     tRNA            complement(6094..6167)
                     /locus_tag="IV334_002361"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6133..6135),aa:Trp,seq:cca)
     gene            complement(6174..6254)
                     /locus_tag="IV334_002362"
     tRNA            complement(6174..6254)
                     /locus_tag="IV334_002362"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6218..6220),aa:Tyr,seq:gta)
     gene            complement(6256..6331)
                     /locus_tag="IV334_002363"
     tRNA            complement(6256..6331)
                     /locus_tag="IV334_002363"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6296..6298),aa:Phe,seq:gaa)
     gene            complement(6367..6439)
                     /locus_tag="IV334_002364"
     tRNA            complement(6367..6439)
                     /locus_tag="IV334_002364"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6404..6406),aa:Asp,seq:gtc)
     gene            complement(6449..6521)
                     /locus_tag="IV334_002365"
     tRNA            complement(6449..6521)
                     /locus_tag="IV334_002365"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6486..6488),aa:Val,seq:tac)
     gene            complement(6537..6608)
                     /locus_tag="IV334_002366"
     tRNA            complement(6537..6608)
                     /locus_tag="IV334_002366"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6573..6575),aa:Glu,seq:ttc)
     gene            complement(6623..6712)
                     /locus_tag="IV334_002367"
     tRNA            complement(6623..6712)
                     /locus_tag="IV334_002367"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6676..6678),aa:Ser,seq:gga)
     gene            complement(6752..6825)
                     /locus_tag="IV334_002368"
     tRNA            complement(6752..6825)
                     /locus_tag="IV334_002368"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6789..6791),aa:Asn,seq:gtt)
     gene            complement(6835..6950)
                     /gene="rrf"
                     /locus_tag="IV334_002369"
     rRNA            complement(6835..6950)
                     /gene="rrf"
                     /locus_tag="IV334_002369"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            complement(7044..>8404)
                     /locus_tag="IV334_002370"
     rRNA            complement(7044..>8404)
                     /locus_tag="IV334_002370"
                     /product="23S ribosomal RNA"
BASE COUNT         2404 a         1972 c         1447 g         2581 t
ORIGIN      
        1 cctgaaaata agttttctaa atcataggag caacaaaaaa attcgataat taacaaaaat
       61 caaaatgatt aaatctagtg attgaaacat atcatttcaa aaaaattttg taaattcacc
      121 cataaatatc accctattta acttttttca tcgtacacta aaattcaaaa caactatctt
      181 taatctttac ttcttggttt tgttttaata aatttccttc aaaaatgcct ggagaaacta
      241 caatgatcac cgtatctttt tgtgcttgct ccaacttctt caaatctata atagctaaca
      301 gtgtcccttc ttttacctcc tggttacttt caacccgtat ctgaaacgga cttccttcga
      361 gttcaactgt atctaaaccc atatggatca gtatttttct gccagattca ctttcgatcg
      421 ttatggcatg tcgggtagga aatatattat cgattattcc tttaactggc gcatggatat
      481 atccatcatg attcgttata gcgtatccaa ctcctatcat ttttgaagaa aagacctcgt
      541 cttttactgc tgttaatgga ataatttgac cactagctac agcttttaga atgtttcttg
      601 ttttaaagaa atgcactttt tatcacctct actttaatgc tggccaatat tttttgttta
      661 ctttgatcat ttcatctagt attttttttg cgactctggc atttggtacg gtttgattca
      721 atgtaaatgc ttgtaaagct ttttgataag aaccttcaaa gtaagcatct actagtaact
      781 tctctactgc aacttgtgcc tccatcaatc ctttatgaaa atcactgatt ggttttctta
      841 gtgtaattgg ctccacacca gttgcaccaa catatgccgg tatttcaaca actgcatctg
      901 accgtagatt cggaatcgcc ccttgattag gaacgattag cataaagcga cgacgatcat
      961 cattcaacaa ggaaatagct atatcgacga tatattgtcc atgctcccca aaattaaagt
     1021 tcaatacttc ttccacaata ccgcggcgta ttttatcagc catttctttg gtatttttct
     1081 ctcgtccatc catgaccata ttagcacgtg tatactcttt gtcagtatgc tcaacgacca
     1141 tatcaggata taagtaatac tctaagtaat tattcgggat attacttgga aaatttttcg
     1201 tgatgactga catcatttga aaggtttttt gccaagtttt atctcctata ttaaaatcgg
     1261 cgacttgcat ttcttttgtt attagttttt caataatctc tggcataatt tctcgttgca
     1321 attctttatc ataaatcgat gtataccagc caaagtggtt taaaccataa tacgtaacga
     1381 tccaattctt acgatcataa ccgtaattca ctgcaatcgt ttcttcaatt gaaatagtca
     1441 tatcacaggc attaatcatt tttacattcg gatatttacg acgaacagct tcagaaacga
     1501 tcgactctgg atttgtataa ttcaaaatcc atgctttggg ggcatagtct tgtatatgat
     1561 cgacaatttc caataaacct ttgattgagc gcatcccata agcaaatcct ccgagtccac
     1621 acgtttcttg tcctactaat ccatgtttaa gtggaatctt ttcgtccttt tctcgcatct
     1681 ccaatcctcc aacacgaatt tgtgagaaaa caaaatcgca tcctgtaaaa gcaagctttg
     1741 gatcatccgt tgcacgaatg cgaatatttg aaaaaccttt tcttttcagc ataaatttaa
     1801 caatcaagta catatccata tttctttctt catcaatatc atataaacgt atttcagata
     1861 atggcaaccg tttttcattg ttcaaaatgg cttgaacgat tcctggtgta tacgtactcc
     1921 cgcctccagc tatagtgata atctgttttt tcatcatcat cttttccttt ctattcttct
     1981 aataatttat tgaattctgc cataacataa gtcacttcta agccgatgat aatttgataa
     2041 tttttttcag ttggtttaat gatcccgctt atccctaatt gcttgattcg ctgttcctcg
     2101 accatggtgg gatccactac ctctacccgt aggcgtgtat tacaactata gacatccagg
     2161 atattggctt ttcctccaaa cgcttgtagc atttttgttg ccatatattc ataattttta
     2221 cttgctaacc ctaactctgt ttcttctaag gttacttcat tgccaaactc tgccttagct
     2281 tcacgtccta atgtctgaat gtttctctta ttgatcaaat aataaaaagt aacgtaatac
     2341 aaggcaaaaa agaaaattcc gattggaaga atcaaaatag cattggtagc gattcgaaaa
     2401 ttgatgagat aatcaacaat acatgctccg aatgtaaaac caattcgtat attgaataag
     2461 taacagacaa tccctgctaa accggcaaaa agtgcatgga tcacgtacaa cataggagaa
     2521 agaaacatga aactaaattc aacaggctca gtgatatttg caataaatga agttcctgca
     2581 ccgcttccca tgattccttt gaccatctct ttattttctt tataagctgc tttatagata
     2641 gctagacata tagctggtat accaaacatc attactacaa aaaaaactga taaaaacagt
     2701 ccagcctgag gatctccgtt gataaagcgt gtcatctctc ctctgaaaac ttccttgcta
     2761 ggagatgtat agcttcctaa atcgtaataa atatacgtat tcaacacatg gtgcagtcca
     2821 aaggggatca gcaaacgatt caaaaacata aatatcccca cacctaatgc tcccatatca
     2881 acgagcagtt ttgcaaaatt atcaatccca atttggatga gcggccagat aaagcccaaa
     2941 aaaacagctg cgattgtttg tagaatcatc accatagtca atgggaattt ttcgcccgca
     3001 aaataagaaa aaactactgg taatttttgt tctttaaagc gattatacgt ccatgccgct
     3061 actaaaccgg aaataattcc accaaaaatc cccatattca cttctgggtc cataatttct
     3121 aagccttttt tcaaggtagc aatcgctaaa aaaccagtaa gtgcaggtat tcctttgtcc
     3181 ttatttttga caaaaccgat tgcaactcct atagcaaata agatatccaa gtttccaaaa
     3241 acggcatttc cagctgcacc aattactggg atgttcagca tatcgtcagc tgataaacgc
     3301 atgatcaatc ccgctaatgg catcgctgca actggaagta gcatcgctct tccaagcttc
     3361 tgcacatttg atttaacatt catttttatt cctcctctat taaatatcct catatgttac
     3421 taactcaatc aaaggatcat ttttcacttt ttgagcaaat gctgaatgaa ttaaaaattc
     3481 gacttggtgg gtacgaggat acacaaaact tgaaccattt aacaattcct gatccaaata
     3541 cgctgcgtga cacatgactt cgatcgtttt atcttctctc aatatcatga gtaaacttgc
     3601 caaatattct tccacactcg tattgatttg tcgttttttt tcatcagttt ctcctacgac
     3661 atcgaaacaa ggctcaaaat aatcgacatg acgaacttca tcttttcgtt caaagttgct
     3721 ccgaaccggc aaatcatatt tttttgctaa tgtaacaact acctcctgat tttcatccaa
     3781 agtatgagta tgatgatgac tgtctaaatg agttggtatg atccccgcat gataaatttt
     3841 ttgaatctga gcattccatt cctgatacag ttgatctttg tcaatgttaa atggatgctg
     3901 ataataagat aaggatctga actctccgct ttcgataaga gtatcgacag ttttgaggag
     3961 tggtttgcca cacgtcaatg ttaaatggac tcctacacct agcctaggca tttcctttct
     4021 tagtttcaca gcatgctcga aacccggcat atttgccatc aatgtagttg atgtcaaaat
     4081 tcctcgctga taagcatcca taatcccata attcacacca tggctatagc caaaatcatc
     4141 cgaattaaaa atcacttttc ccatttcatt ctccttcttt tttctactaa aataggcgca
     4201 accaaataat catagtccat ttactatgtg tcatttggtt gcgcctatag atagttacgc
     4261 ccttatttta atagattctt gctagatgaa ttgccaaata gatgatttca ttttcttgaa
     4321 tggcaagttg aaattgctgt tcgatgatat ctttaatctt tagtgctgta caatacgctt
     4381 tgctatgttt aaatgtttca gtaaaaaaac caatattttc tactggcgtt tcttcctgat
     4441 aaatcaagcg tttaaccagg aatttgatat gaaccattaa gcgatgataa gtcagtgatt
     4501 ctttatcata aacgtttgaa taggattttt ctagatggga caaaatcgtg gtcgtcattt
     4561 ctactatttt taagctcttt aaactattag gctctcctag ctcagcatta atgatatgca
     4621 tagtgatgaa tcctacttca tttgacccta actcggtttg atatcgatga ttgattcgct
     4681 cgaccacttc tactgctata tcatattcat ttggataaag gaatctcatt tctggcaaca
     4741 taattgctgt gacatctcta tcttctcttt ttcgttgaat agcaaaataa atatgatctg
     4801 tcaatgtaat atagatattt gaatctaatg gagaggagat cctagctgtt atcagttcaa
     4861 tcacttcttc cgcaaaatcc ataaattcga ttggaatctg cgagactatc tcactgaatc
     4921 gtttgatctt ttctttagaa tctaatacaa ataatttgtc agcatcctca acatcaaatg
     4981 gcttactcac tttagcagaa aagccaatcc ctttccccat cgcaattttt tctattccat
     5041 cgtcatcgat caatacagca ttatgattca gctttttcat cacatacacc gttaattcac
     5101 ctcctctata tgataaaaca aaacaacttg cagactcctt catacatatt tttgtctcca
     5161 aacaattttt aaagacaaaa aacgacctaa gaaagaaccc tttcttaggt cgtgcctaat
     5221 tgaatagtag caccctacat aatttacaaa aatagtttaa cacataataa aagcgttatc
     5281 aatatctttt ttactcgttt tatgataata cttgtatcga attgatttgg aggtaattac
     5341 ataaaaacaa aaaaaatata gcaactctct ataagaaagt tgctatattg ataattatac
     5401 cggcggccgg ggtcgaaccg gcacgtcctc aacggacact ggattttgag tccagcgcgt
     5461 ctgccaattc cgccacgccg gcaaatttaa aaataataac tggggtagct ggattcgaac
     5521 caacgcatga gggagtcaaa gtcccttgcc ttaccgcttg gctatacccc aagaataaag
     5581 ggcgaatgat gggaatcgaa cccacgaatg ccggagccac aatccggtgc gttaaccact
     5641 tcgccacatt cgccatcata aaagatgtta tttaatttct caataaaaaa ctaagatgaa
     5701 gacatcttag tcaataccgg cggccggggt cgaaccggca cgccctcaac gggcactgga
     5761 ttttgagtcc agcgcgtctg ccaattccgc cacgccggca agttgattta ttagtactaa
     5821 ggcggtaacc ggatttgaac cggtgatgaa ggttttgcag acctctgcct taccacttgg
     5881 ctataccgcc attatactat tatcaatcta taaaataaag aataaattat agaaactggg
     5941 gtagctggat tcgaaccaac gcatgaggga gtcaaagtcc cttgccttac cgcttggcta
     6001 taccccaaga ataaaaaggg cgaatgatgg gaatcgaacc cacgaatgcc ggagccacaa
     6061 tccggtgcgt taaccacttc gccacattcg ccatggcagg ggcagcagga attgaaccca
     6121 cactaacggt tttggagacc gctgttctac ctttaaacta tgcccctata aaatggagga
     6181 gagtggattc gaaccactga accctaagga acggatttac agtccgtcgc gtttagccac
     6241 ttcgctactc ctccatggtg gcgcgggaca gaatcgaact gccgacacac ggagcttcaa
     6301 tccgttgctc taccaactga gctaccgcgc catattttaa aattattaat tgaataacaa
     6361 gaacgacggt tccgacggga atcgaacccg cgatctcctg cgtgacaggc aggcatgtta
     6421 acccctacac cacggaacca gttcattatg gaggttaacg ggatcgaacc gctgaccctc
     6481 tgcttgtaag gcagatgctc tcccagctga gctaaacctc caacattcaa ttaaaatgac
     6541 ccgtacggga ttcgaacccg tgttaccgcc gtgaaagggc ggtgtcttaa ccgcttgacc
     6601 aacgggccag gtaaatttgc tacggagagt aagggattcg aacccttgag acagtttggc
     6661 accatctaca tgatttccaa tcatgctcct tcggcctctc ggacaactct ccagagtgaa
     6721 agaagctcaa tactctttct caaaatgaga actccggcag taggactcga acctacgaca
     6781 tcatgattaa cagtcatgcg ctactaccaa ctgagctatg ccggaataac atcagcgtgg
     6841 cgacgtccta ctctcacaag gggcaacccc tcactacaat cggcgctaag aagcttaact
     6901 tctgtgttcg gcatggttac aggtgtatcc ttctcgctat cgccaccaca ctgtggtgtt
     6961 atcttttatt gagtaaattt tgttcactca aaactggatt tgaagtcaca taagtttttt
     7021 tccgagtact tttcttttaa cctattggtt aagtcctcga tcgattagta tcagtccgct
     7081 ccatacatca ctgtacttcc actcctgacc tatctacctg atcatctctc agggatctta
     7141 ctttcttaaa gaaatgggaa atctcatctt gaggtgggct tcacacttag atgctttcag
     7201 cgtttatccc ttccctacat agctacccag caatgccctt ggcagaacaa ctggtacacc
     7261 agcggtaagt ccatcccggt cctctcgtac taaggacagc tcctctcaaa tttccaacgc
     7321 ccgcgacgga tagggaccga actgtctcac gacgttctga acccagctcg cgtgccgctt
     7381 taatgggcga acagcccaac ccttgggacc gactacagcc ccaggatgcg acgagccgac
     7441 atcgaggtgc caaacctccc cgtcgatgtg gactcttggg ggagataagc ctgttatccc
     7501 cagggtagct tttatccgtt gagcgatggc ccttccatgc ggaaccaccg gatcactaag
     7561 cccgactttc gtccctgctc gacttgttgg tctcgcagtc aagctccctt ctgcctttac
     7621 actcttcgaa tgatttccaa ccattctgag ggaacctttg ggcgcctccg ttacctttta
     7681 ggaggcgacc gccccagtca aactgcccat ctgacactgt ctcccaccac gattagtggt
     7741 gcgggttaga gtggccataa cgcaggggta gtatcccacc agcgcctcca tcgaaactag
     7801 cgttccgatt tctacggctc ctacctatcc tgtacatgcg gtacagacac tcaatatcaa
     7861 actacagtaa agctccatgg ggtctttccg tcctgtcgcg ggtaacctgc atcttcacag
     7921 gtactaaaat ttcaccgagt ctctcgttga gacagtgccc aaatcgttac gcctttcgtg
     7981 cgggtcggaa cttacccgac aaggaatttc gctaccttag gaccgttata gttacggccg
     8041 ccgtttactg gggcttcaat tcgtaccttc gcttgcgcta agcactcctc ttaaccttcc
     8101 agcaccgggc aggcgtcagc ccctatactt catcttacga ttttgcagag acctgtgttt
     8161 ttgataaaca gtcgcttggg cctattcact gcggctgatc gtatgatcag caccccttct
     8221 cccgaagtta cggggtcatt ttgccgagtt ccttaacgag agttctctcg ctcaccttag
     8281 gattctctcc tcgactacct gtgtcggttt gcggtacggg ccgctgtttt ctcactagaa
     8341 gcttttctcg gcagtgtgac atcaggaact tcggtactat tatttccctc cccatcacag
     8401 cttg
//
LOCUS       DACDKM010000110         8308 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20834_2016
            SAMN12640370-rid9891663.denovo.110, whole genome shotgun sequence.
ACCESSION   DACDKM010000110 DACDKM010000000
VERSION     DACDKM010000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640370
            Sequence Read Archive: SRR10040891
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8308)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8308)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742835.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742835.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:04:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,012
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 29 of 75
            Pseudo Genes (incomplete)         :: 33 of 75
            Pseudo Genes (internal stop)      :: 19 of 75
            Pseudo Genes (multiple problems)  :: 5 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8308
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640370-rid9891663.denovo.110"
                     /isolate="E20834_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            351..1400
                     /locus_tag="IV334_002371"
     CDS             351..1400
                     /locus_tag="IV334_002371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAP8944401.1"
                     /translation="MKNKRLITVVVIILVYLAGAFIYGKASGEKESTSTNQTKKTTVV
                     GVLQYVSHPALDEIYRGIKDGLKEEGLKEGENLEIKFQNGQADQSKLATMSQQLVQSD
                     PDALIGIATPAAQSLANTTSSIPVVLGAVTDPVGAGLVASLEKPGGNVTGVSDQPPVT
                     SQIKLASDLLPNAKKVGILYASSEDNSKYQVAQAEEAAKKEGLESVKYAVPSTNEIAQ
                     TVQVMSRQVDFIYVPLDNTIANAMQTVVKEANKANIPVIPSVDTMVEQGGLATIGINQ
                     YQLGLQSGKMAAELASGKEKPETTPVYTFDQGDTVINQSQADRLGIIVPQSMKEKAKI
                     ITDDSQQETSKEDDK"
     gene            1397..2278
                     /locus_tag="IV334_002372"
     CDS             1397..2278
                     /locus_tag="IV334_002372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP8944402.1"
                     /translation="MIVSAISQGMLWAILGLGIFMTYRILDFPDMTTEGSFPLGAAVC
                     VTAITHGVAPIVATLLGVGAGMIAGLVTGLLYTKGKIPVILAGILVMSGLNSVILFVM
                     RTPNLSLLNHPILQDSFHRFGLPDYFDTVLLGFISLGILIAVLLFFFNTDLGQGYIAT
                     GDNETMARSLGIKTDRMKILGLTLSNGVIALSGALIAQNDGYADVNKGIGVIVIGLAS
                     IIIGEVIFQELTLAERLIAIVVGSIIYQLLILVVIKLGFDTTYLKLFSAIILAICLMI
                     PQLKKALNLKTGFEKEA"
     gene            2281..3051
                     /locus_tag="IV334_002373"
     CDS             2281..3051
                     /locus_tag="IV334_002373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAP8944403.1"
                     /translation="MTDILTIKNGRKVVNNGMNEEKVLLDEINLTIREGDFITVLGGN
                     GAGKSTLFNTIAGTLQLSQGSISFKDQLITKDSEEKRARFLSRVFQDPKMGTAPRMTV
                     AENLLLAQKRGQKRKFRLRNLKNQKEDFFRLCQEVGNGLEQHLDTPAGELSGGQRQAL
                     SLLMATITAPELLLLDEHTAALDPKTAKALMRLTNQRINEQKLTCLMITHRMEDALKY
                     GNRLIVLQKGKIVQDISEKEKQHLTMGDLLQFFEEVVD"
     gene            3330..4184
                     /gene="aroE"
                     /locus_tag="IV334_002374"
     CDS             3330..4184
                     /gene="aroE"
                     /locus_tag="IV334_002374"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HAP8944404.1"
                     /translation="MISGQTKLAGLFADPASHSLSPLMHNTAFEANNIDAVYLAFQVN
                     PMNLKQAVESIRTFDMLGVNLSMPNKTAVIPYLDEVSQEAQLIGAVNTIVHEKGRLIG
                     YNTDGAGFMQSVQEAGISIKKKKMTLLGAGGAAKAVTVQAALDGAEEIVVYKRNNQTF
                     AQVKETFEQLSYRTSCKIKVRDFADTHQLRKDLKESCLLVNATDIGMGKKINQSPLLD
                     ISMMHENLAVFDLIYAPRETRLLKEAKKVGAKGYNGLGMLIYQGAIAFELWTKEKMPI
                     HVIEKLFD"
     gene            4362..5525
                     /locus_tag="IV334_002375"
     CDS             4362..5525
                     /locus_tag="IV334_002375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HAP8944405.1"
                     /translation="MSRMDRYKDIHKKAKPLKRDTGSIFARRERKRTRMENEEDATRI
                     YEREQHSFEHLNQYEQQIYRDAPRAEEQQKNKKFFNRQKKNKPLKPRRKRSWKKIILS
                     ILLVLILYSGISFFIGKYVAEHDTSLEAATSETFHGQKATNGAHNILILGSDTRGEDA
                     GRADTIMVLQLDGPSHKPKLISFMRDSFVTIPGVGQNKINSAYAYGGADLVRQTLVEN
                     FGIDCQYYAKVDFKSFEKVIDALFMNGVKIDAEKDLNLDGVDIKKGVQKMDGHVLLQY
                     ARFRMDEQGDFGRVRRQQQVMNAIFSQLKNPLNLIRAPYAAGKALGYTSTDVTAWFLT
                     KNLFSIARGAGGIDRISVPVDGSWNYGQSDYAGSILEIDNQMNQSAITDFLNK"
     gene            5642..6052
                     /locus_tag="IV334_002376"
     CDS             5642..6052
                     /locus_tag="IV334_002376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HAP8944406.1"
                     /translation="MDIYLNFKNESREAIAFYEKVFQTEASGITTYGEMPGIEQMNLP
                     EEAKNLITNARLNIYDTLVMFADTPEGIGPELVVGNNVSLVIQTRNEEETKRLFESLA
                     EGGKIEMPLDAVPWAQLYGMVTDRFGINWQINQE"
     gene            6226..>8308
                     /gene="topB"
                     /locus_tag="IV334_002377"
     CDS             6226..>8308
                     /gene="topB"
                     /locus_tag="IV334_002377"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HAP8944407.1"
                     /translation="MKTVILAEKPSQAKAYAETFQQQKRHEGYYEISDPLFAGEVTIT
                     YGFGHLVDMVAPGAYEERWAKWSLENLPIFPETFRYEVPMDKRAQFSIVKRELQSADT
                     IIIATDGDREGEAIAWSIIRQANAFSKEKNYLRLWINSLEKEAIYEGFKNLQPGKNYF
                     PKYKEAQARQNADWLIGMNGSPLYSLLLQRKGIDGSFSLGRVQTPTLYMIYQLQEEIK
                     HFKKETYFEGEGLITTNKGQFSGKIVPKKAFKTQEELTKEIEKLGAHLGKQNGRILEV
                     TKKEKRLHSPRLFSLSSLQTKMNQLMKASAKATLDAAQGLYESKFLSYPRTDSFYITE
                     NEHQYLVKHLQNYKKFLGIEEVQTTEIKAKKRYVDAKKVQEHHAIIPTKTIPTPARFA
                     KLSKLQQAIYLQVLRTTVAMFAADYIYEETTVMTGVQQLKLKSIGKIPLQQGWQEILR
                     IKSKKKEAQTLPPIVENEEAEVELKSMEKETQPPKPYTEGTLITAMKTAGKTLDNDEA
                     QEILKEVEGIGTEATRANIIENLKQKHYIEVNKNEITVTPKGITLCKAVANEPLLTSA
                     EMTARWEGYLRKIGKQEGTPSVFLENIKKFILHLLAEVPNQIDKVDFSDEISGTRQIK
                     AMEKKNDQLGRCPKCKKGIVMLYPKIATCLNPECDFKLWPTVAKKKLTKTNLRDLLSK
                     GKTSKVVKGFTG"
BASE COUNT         2851 a         1445 c         1808 g         2204 t
ORIGIN      
        1 atatagttga cgaaattcaa aaattcgatt agacttaaat caattcttaa ggaaagggtg
       61 acctgtaatg aacttatcaa aacgtatttc agcagaaaac aaaaaaaatt ggcgcagctg
      121 gcgtagataa gttgtattaa tggtgagact ttcccataga tacaactttt tagagtgcat
      181 ttctaaagct tgtatctatg ctagacatga acattgactg ccgcatagat tagaatctat
      241 gcggtttttt gtgtaaagac aatgaccgct ttggccattg tctttttttg tagactggat
      301 acttgcctga ttataataaa taaaaagatt gactttgcag gaggaaaaaa atgaaaaaca
      361 aacgattaat aacagttgta gtaattattt tagtttattt agcaggtgca tttatttatg
      421 gaaaagcctc aggcgaaaaa gaaagtactt cgacgaacca gacaaaaaaa acaacagttg
      481 ttggggtatt gcaatacgtg agccatccgg cattagatga gatttatcga ggaatcaaag
      541 atggtctgaa agaagaagga ttaaaagaag gagaaaatct tgagatcaaa tttcaaaatg
      601 gacaagccga tcagagcaag ctagccacaa tgagtcaaca gctagtacaa tctgatccag
      661 atgccttgat tggaatcgca acacctgccg ctcaatcatt agcaaacacg acttcatcga
      721 tcccagttgt gttaggtgct gtaacggatc ctgtgggggc tggattagtc gcttctttag
      781 agaaacctgg tggtaatgta acaggtgttt ctgatcagcc gcctgttact tcacaaatca
      841 agcttgcttc tgatttgttg ccaaatgcaa aaaaagtcgg aatcttgtat gcttcttcag
      901 aggataattc aaaataccaa gtagcacaag cagaagaagc tgcaaaaaaa gaagggttag
      961 aatcagtaaa atatgcggtg ccttccacta atgagattgc acaaacggtt caagtgatga
     1021 gccgacaagt cgattttatc tatgttccat tagataatac cattgccaat gcgatgcaaa
     1081 cagtggtaaa agaagcaaac aaagcaaata tccccgtcat tccatctgtg gatacaatgg
     1141 tggaacaagg tggactagca acaatcggga tcaatcaata tcaattaggg cttcaaagcg
     1201 gtaagatggc ggcagaactt gcgagtggaa aagaaaaacc ggaaacgacg cctgtttata
     1261 catttgatca aggggataca gtgatcaatc aatctcaagc tgatcgctta ggaatcattg
     1321 ttccccaatc gatgaaggaa aaagccaaaa tcattacaga tgactcgcag caagaaacat
     1381 ctaaggagga tgacaaatga tcgtatcagc tatttcacaa ggcatgcttt gggcaatatt
     1441 aggattaggg attttcatga cttatcggat cttagacttt cctgatatga cgacagaagg
     1501 gtccttccca ttaggtgctg ctgtctgtgt gacagctatt actcatgggg tagcgccgat
     1561 tgtagctaca ttattaggcg taggtgctgg catgatagca ggactcgtta cggggttact
     1621 ttatacaaaa ggaaagatac cggttatttt agcagggatt ttagtgatgt caggattgaa
     1681 ctcagttatt ttgtttgtga tgcgaacacc aaatctttct ttattgaatc atccgattct
     1741 acaagacagc ttccatcgct ttgggttacc tgattacttt gacacagtcc ttcttggctt
     1801 catctctttg ggcatcttga ttgctgtact gctattcttc ttcaatacag atttgggtca
     1861 aggctatatt gcgacaggag acaacgaaac gatggctcgg tcgttaggga taaaaaccga
     1921 tcgtatgaaa attttaggtt taactttgtc gaacggtgtc attgcattat ccggtgcgct
     1981 gattgcccag aacgacggat atgcagatgt aaataaaggg attggcgtaa tcgtcatcgg
     2041 tcttgcttcc atcatcatcg gtgaagtgat cttccaagaa ttgacgctag cagaaagatt
     2101 gattgctatt gttgtcggca gtatcattta tcagctattg attttagtag taatcaaact
     2161 agggtttgac acgacttatc tgaagttgtt ctccgctatc atcttagcta tttgtctgat
     2221 gatcccgcaa ttgaagaaag cactgaactt aaaaacgggt tttgagaagg aggcatagta
     2281 atgactgata tattgacaat caaaaatggc agaaaagtcg taaacaatgg aatgaatgaa
     2341 gagaaagtac tattggatga aattaacctt acaatcagag aaggggactt catcactgtc
     2401 cttggcggga atggtgctgg aaaaagcaca ctgttcaata cgatcgctgg tacgcttcaa
     2461 ctcagtcaag gatcgatctc ttttaaagat cagctgatca caaaagattc agaagaaaaa
     2521 agagcacgat tcctttctcg agttttccaa gaccccaaga tgggaacggc tcctcggatg
     2581 acagtagcag aaaatttgct acttgcacaa aaaagaggtc aaaaacggaa attccgttta
     2641 cgtaacttga aaaatcaaaa agaagatttt ttcagactat gtcaagaagt cggtaatggc
     2701 ctagagcaac acttggatac tccagcgggt gaattatctg gtggtcaacg ccaagcatta
     2761 agcttgctga tggcgacgat cacagctcct gagttactat tattagatga acatactgca
     2821 gctttagatc ctaaaacagc taaagcatta atgcgattaa caaatcagcg gatcaatgaa
     2881 caaaaattga cttgtctcat gatcacgcat cgaatggaag acgccttgaa atacggaaat
     2941 cgtctgatcg tactccaaaa agggaaaatc gttcaagata tttctgaaaa agaaaagcaa
     3001 catttgacaa tgggcgacct tttgcagttc tttgaagaag tagtggattg atttcgacag
     3061 aaaaattcct ttcagtagaa ggacttgaaa actcattgac atcttccttt gattcatcct
     3121 atacttaaaa caataaacaa ttaaacatat ttgggtgttt tgtaggatca cgtagtcaat
     3181 gattttaaca attaaataga atattctttg ctctgacaag taaacgatga gaaaagaatt
     3241 tgctactttg ccacctagac gtttcgtact ttttcgtatc aaacgctaag tggctttttt
     3301 tattgaagta agaacaaagg aggaataaga tgatttctgg acaaaccaaa ttagctgggc
     3361 tctttgctga tccggcttct cacagtttat ccccattgat gcacaatact gcttttgaag
     3421 caaacaacat cgatgcggtg tatttagctt tccaagtaaa tccaatgaat ttgaagcaag
     3481 cagtcgaaag tattcgaact tttgatatgt taggtgtaaa tctttcgatg ccgaataaaa
     3541 cagcggttat tccttatctg gatgaggtaa gccaggaagc gcaattgatc ggagcagtga
     3601 atacgatcgt gcacgaaaag ggtcgtttga ttggctataa taccgatggt gcgggcttca
     3661 tgcaatccgt gcaagaagct gggatttcga tcaaaaagaa aaaaatgact ctactgggag
     3721 ctggaggtgc agcaaaggca gtcactgttc aagctgcgtt agacggcgct gaggaaatcg
     3781 tagtttataa acgaaacaac caaacattcg ctcaagtaaa agaaacattc gaacaactca
     3841 gctatcgaac ttcttgtaag ataaaagttc gtgactttgc cgatacgcat caattaagaa
     3901 aagacttgaa agaaagctgt cttctagtca atgcaacaga tatcgggatg ggaaagaaaa
     3961 tcaatcagtc accgctctta gatataagca tgatgcatga gaatctagcg gtttttgatt
     4021 tgatttatgc acctagagaa acacgtttat taaaagaagc aaaaaaagta ggagcaaaag
     4081 gatacaatgg acttggcatg ttgatctacc aaggagcaat cgcgtttgaa ttatggacaa
     4141 aggaaaagat gccgatccat gtgattgaaa agctgtttga ttaaaaaagg ataatagata
     4201 aagatctttt attctcgcat aaacggtctc aagaaaagta atcctttatg tatcgaagca
     4261 tttatttcgt tattttttat taaattatca aaaaatgata ggcttttgtc gattggttat
     4321 cgtatccttt atttatcagt aagaaacgag gaggaaccac aatgagtcgg atggatcgct
     4381 ataaggacat ccataagaag gcgaaacctc tgaagcggga tacggggagt atttttgcta
     4441 gaagagaacg aaaacgaacg cgtatggaaa atgaggaaga tgctacccgt atttatgaaa
     4501 gagagcagca ttcatttgaa catttgaacc aatacgagca acagatatac agagatgcac
     4561 caagagcaga agaacaacaa aaaaataaaa aatttttcaa tcggcaaaag aagaacaaac
     4621 cgttaaaacc tcgaagaaag cgatcgtgga aaaaaatcat tctatcgatt ttgcttgttt
     4681 tgatccttta ttcaggtatc tcatttttta ttggtaaata tgtagctgaa catgacacaa
     4741 gcttagaggc agcgacttca gaaacctttc atgggcaaaa agcgacgaat ggtgcgcata
     4801 atattttgat ccttggaagt gatacacgcg gagaagatgc cgggcgagcc gatacgatca
     4861 tggtcttaca gttggatggt ccttcccata agccgaaact gatttctttt atgcgtgaca
     4921 gcttcgttac gatcccaggg gtaggacaaa ataagatcaa ttcagcttat gcatacggag
     4981 gagccgatct agtgcgtcaa actttagtag aaaattttgg aatcgattgt caatactatg
     5041 cgaaagtcga tttcaaatcg tttgaaaaag tgatcgatgc cttattcatg aacggagtga
     5101 agatcgatgc agaaaaagat ctgaatctgg atggtgtaga tattaaaaaa ggggtccaga
     5161 agatggatgg acatgttctt ttgcagtacg cacgtttccg aatggatgaa caaggagact
     5221 ttggccgtgt cagacgccaa cagcaagtaa tgaacgctat tttcagccaa ttgaagaatc
     5281 cattgaatct gatccgtgca ccttatgcag ctggtaaagc tttaggatat acatccacag
     5341 atgtcactgc ttggttcttg acgaaaaacc tcttttcgat tgcacgagga gctggtggca
     5401 ttgatcgaat aagtgtgcca gtagacggtt cgtggaatta tgggcaaagt gattatgctg
     5461 gcagtattct tgaaattgat aatcaaatga atcaatctgc tattacagat tttttgaata
     5521 aatgatcata aaaaagatca agaagaagct gcgcaaggct tttgtcatga atcaatcaaa
     5581 cgattcactc gtaaaaaacg ctttcttatg gtagatttaa ccaagagagg agctgtttac
     5641 attggatatc tatctgaact tcaaaaatga gtcgcgtgaa gccatcgctt tttatgaaaa
     5701 agtttttcag actgaagcat caggtatcac cacatacggt gaaatgcctg gaatagaaca
     5761 aatgaacttg ccagaagaag caaaaaatct aatcacgaat gcacggctga acatttatga
     5821 tacattagtt atgtttgcag atacgccaga aggaattggt cctgaattag ttgtgggaaa
     5881 taatgtttct ttagtcatcc agacaagaaa tgaagaagaa acgaagcgcc tatttgaaag
     5941 cttagctgag ggtggtaaga ttgagatgcc actggatgct gttccgtggg ctcagttgta
     6001 tgggatggta actgatcgat ttggcatcaa ctggcagatt aaccaagagt gacagctaaa
     6061 gatgaatatt ttctggaaac atcaagcagc aaacggatca tcgtttgttt gcttgatgtt
     6121 tttgtgtcta tttcgactat acggtatctt ttttttattg ccagatgcga aaatatgttc
     6181 cataatagag aagtccgcaa aaaattaagt aacggaggtc agaagatgaa gacggtgata
     6241 ctagctgaaa aaccttcaca agcaaaagcc tatgccgaaa ccttccaaca gcaaaagcgg
     6301 catgaagggt attatgaaat cagcgatcca ttgtttgctg gtgaagtaac catcacttat
     6361 ggttttggac atttggtaga tatggttgct ccaggagctt atgaagagcg ttgggcaaaa
     6421 tggtctttag aaaatttgcc tattttccca gaaaccttcc gttacgaagt tccaatggac
     6481 aaacgtgcac aattttcgat cgtaaaacga gaattgcagt cagccgatac catcattatt
     6541 gcaacagatg gtgatcgaga aggtgaagcc atcgcgtggt cgattattcg acaagcaaac
     6601 gcattttcta aggaaaagaa ctatttacgt ttatggatca attctcttga aaaagaagca
     6661 atctatgaag gttttaagaa tctccaacct ggaaagaact attttccaaa atataaagaa
     6721 gcgcaagcaa ggcagaacgc tgattggctg attggaatga atggaagtcc attatatagc
     6781 ttgctacttc aacgaaaagg gattgatggc agtttttctc ttggtcgtgt acaaacaccg
     6841 acactctata tgatttatca gctgcaagaa gaaatcaaac acttcaaaaa agaaacgtac
     6901 tttgaaggag aaggtttaat tacgacaaac aaagggcagt tttctggaaa aatcgtacca
     6961 aaaaaagcat ttaaaaccca agaagagtta acgaaggaaa tagaaaaact tggagcgcat
     7021 ttaggtaagc aaaacggacg gattcttgaa gttacgaaaa aggaaaagag gttgcatagt
     7081 ccacgtttat tttcgctatc tagtttgcag acaaagatga atcagctaat gaaagcaagt
     7141 gccaaagcta cacttgatgc tgctcaagga ttgtacgaaa gcaagtttct aagctatcca
     7201 agaacggatt ccttttacat tacagaaaat gagcatcagt atctggttaa acatctgcaa
     7261 aattataaaa aatttttagg aatagaagaa gtacagacaa ctgagatcaa agcaaagaaa
     7321 cgatatgtcg atgcgaaaaa agttcaagaa catcatgcaa ttattccgac aaagacgatt
     7381 ccgactcctg cacgctttgc gaagctttcc aaattacagc aagcaatcta tttgcaagtt
     7441 ttgcgcacta ctgtggctat gtttgctgca gattatatct acgaagagac gaccgtaatg
     7501 acgggcgtac agcaattaaa gctaaaaagc atcggaaaga tcccattaca gcaaggttgg
     7561 caggaaatct tacgcatcaa gtcaaaaaag aaagaagctc agactcttcc tcctatcgta
     7621 gaaaatgaag aagcagaagt agaactgaaa agtatggaga aagaaacaca acctccaaaa
     7681 ccctataccg aaggaacgtt gatcacagca atgaaaactg cgggaaaaac gctggataat
     7741 gatgaagcgc aagaaattct caaagaagta gaaggaatag ggacagaagc tacacgagcc
     7801 aatatcattg aaaatcttaa gcagaagcat tacatcgaag tcaataaaaa cgaaataact
     7861 gttacgccaa aaggaatcac tctttgtaag gcagtagcca atgaaccatt actgacgagc
     7921 gcagaaatga ctgcgcgctg ggaagggtac ttgagaaaaa ttggtaaaca agaggggaca
     7981 ccatctgttt ttttagagaa tatcaaaaaa ttcatcctac atttattggc agaagttcct
     8041 aatcaaatcg acaaggtaga cttttcagat gagattagcg ggacacgtca gataaaagcg
     8101 atggagaaga aaaacgatca attaggaaga tgtccaaaat gtaaaaaggg aatagtcatg
     8161 ctttatccta aaatcgctac atgcttgaat ccggaatgcg acttcaaatt gtggccaaca
     8221 gtagctaaaa agaagctgac taaaacgaat ctcagagatt tgctttcaaa aggaaagaca
     8281 agtaaagttg tcaaaggttt tactggta
//
LOCUS       DACDKM010000111         8058 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20834_2016
            SAMN12640370-rid9891663.denovo.111, whole genome shotgun sequence.
ACCESSION   DACDKM010000111 DACDKM010000000
VERSION     DACDKM010000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640370
            Sequence Read Archive: SRR10040891
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8058)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8058)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742835.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742835.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:04:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,012
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 29 of 75
            Pseudo Genes (incomplete)         :: 33 of 75
            Pseudo Genes (internal stop)      :: 19 of 75
            Pseudo Genes (multiple problems)  :: 5 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8058
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640370-rid9891663.denovo.111"
                     /isolate="E20834_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            278..787
                     /locus_tag="IV334_002378"
     CDS             278..787
                     /locus_tag="IV334_002378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAP8944408.1"
                     /translation="MVQGIERGVYVVDKIQFNQVIEEFIKVNNLMTHIQKRPIRITND
                     LKLSTSMIHLIDVIGNYPETSITELADRLGVTKGAVSQQIPTLEKMNLISISQKKGNK
                     KTKLVSLTKYGRKVYESHNYLHEELYSSINEALESFSPDQISTIKQILEKVSYTINEY
                     QVSLTRKED"
     gene            880..2042
                     /locus_tag="IV334_002379"
     CDS             join(880..1156,1156..2042)
                     /locus_tag="IV334_002379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HAP8944409.1"
                     /translation="MSRRQRRTYSKEFKQQVVDLYLAGKPRAEIIRDYELTTSAFDKW
                     VKQAQTTGSFQEKDNLIPEQVELIALRKKNKQLEMENDILKQAALILGPKRQVIDTNK
                     HKYSISAMCKVLNISCQTYYYQAKPVEDESELEELVQEEFIRNRKAYGTRKLKQCLAN
                     LGLQVSQRRIGRMMKQRGLRSTYTLAHFKFHSSTCNEAKTANILDGTFLQEKSLEAIV
                     TDLTYVRVGKKWQYICLILDLFNREIIGYSCGEKKDATLVKEAFAKIPYSLNNVQLFH
                     TDRGKEFDNQTIDEILWAFNITRSLSKKGCPYDNAVAESTYKSVKVEFVHQYRFETLA
                     NLRLELFDYVHWWNTIRLHGTLGYETPISFRQQRLAQRILDNELGCDTGGEAP"
     gene            2322..3302
                     /locus_tag="IV334_002380"
     CDS             2322..3302
                     /locus_tag="IV334_002380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016179831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAP8944410.1"
                     /translation="MKKIGIREVAKEANVSPTTVSRVLNDRGYLSEETKRKVFDAMKK
                     LEYYPNELARALFKNETYTVGLIFPTITNPFHAELIQDIEFELSSRGYKVLLCNSLNN
                     PTKEKEYLTMLRKNQVDGIIVGTHNKDIADYDIPGLPIVAIDRSLGENTVTVSCDNYR
                     GGELATQLLVNHGCQNILCIRGDSKIKLPANSRTLAYKDVLNKFMLEPLVLEVPFVKE
                     INEKNEIIKTYLETHPNIDGVFAGDDLLASLAINNLGSSGKRVPKEVKVIGFDGAQQT
                     LIYNPELSTIQQPIDQISKVAVKKLLNMIKGEKETDELSLPVKLIKKNTA"
     gene            3424..4842
                     /locus_tag="IV334_002381"
     CDS             3424..4842
                     /locus_tag="IV334_002381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-fructofuranosidase"
                     /protein_id="HAP8944411.1"
                     /translation="MEKLLIKSNNHQLYYQPKDVWVGDVMPYGKEGKFFIYHQRDTRN
                     PVPFGEPFGWALATTKDFVHFEDFGESLLRGDDNERDQFIYAGSVFEANGKVHAFYTG
                     YNREFLAQGKTSQVLLHAESDDFVHWEKSETALELEPQEGYDIRNWRDPFVVWDDEKE
                     EYLLILGARKGQDKHLQTGRLVEFTSKDLTNWEFKGDFWAPGLYTMFEMPELFKIGDW
                     WYLVYSEYSDGNKILYRMSQSLEGPWIKPKDEAFDGRAYYAGRTAYDGKRRVLFGWVP
                     TKDQENDKNNYLWGGTLVPHEVYQKEDFSLGVKPIDEVWNAFINLHDIADVEIETADT
                     REEVTLVKNSGTLFNLETTISFDENLSDFSLRLYKNLETDESYEYRFNLDEGRLTFDK
                     NPCYPWFQYMDKGLIRPISLKPNQSYKLKVIVDDNIMTLYIDGIALNARVHGCFGQAI
                     TITATNGSMTAINTKFSNEIKK"
     gene            4913..6382
                     /locus_tag="IV334_002382"
     CDS             4913..6382
                     /locus_tag="IV334_002382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016179833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP8944412.1"
                     /translation="MDYKKIAEDVLENVGGKENISSATHCITRLRLILKDKSKANMEM
                     LEEIEGAKGVLFNSGQLQVIFGTGIIEKVYKEFVELTGAKEVSVSEMKDEGVNNSGNR
                     LQRAFKVFSDIFIPIIPAFIGAAMVLGIRSLITTPGMFGMDGSFADHNVFLNDLSKFL
                     GIIATTFDYLPVLVMYSATKRFGGNPILGLLVGFVMIHPGLANRNDFVMGTIKPDYWH
                     LFGLSIPAVAFQGGVFPAILTSWFLAKVEKITNKYSPQVLAYILVPTVTIVLANFALF
                     LVFGPLGNFIGNVLGGIIDFLYMKMGAFGAFVFAGALQPLVVTGTQHAIQGIEANLVA
                     TTGFNYIQPIWSVSIIAQGGGCIGMYLLFKKKSKDRDIAISSFIPTLVGITEPAIFAV
                     NLKYSIVPFVCSFIGAGFGGAFMKIFDVKAIGQGLTVLPGLTIANPVLPYIFGNLIAF
                     ILPIIFILAYDKYKVFIPEAKHRKFAEKTDSKKLPVQVK"
     gene            6392..6859
                     /locus_tag="IV334_002383"
     CDS             6392..6859
                     /locus_tag="IV334_002383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HAP8944413.1"
                     /translation="MLNFFRKNEISVVAPATGVFEKLEKLSDPVFSKGMMGQGFAIDA
                     KNETIVSPIDGFVSSIFPTKHALGIKTKSGIEILVHVGIDTVELNGEGFDIKVQEGQK
                     VKAGDILMTINFVVFEKNHKTKDVIVVFPDYQDEIFIPKLEEVKQGQELFVLG"
     gene            6895..7767
                     /locus_tag="IV334_002384"
     CDS             6895..7767
                     /locus_tag="IV334_002384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HAP8944414.1"
                     /translation="MVFGAIEAGGTKFVCAIGDKDLNIFEKVSFPTTTPKETMNLVLD
                     FFNHHKDDLEGIGVGSFGPIDIHCESKTYGFITSTPKLAWQDFDFIGSLKKQLNIPTA
                     WTTDVNASCYGEYVSGNGRGLSSVVYYTVGTGIGGGAIQNGNFIEGFSHPEMGHMLVK
                     RHNKDNFSGTCPFHGDCLEGLAAGPAIERRSGKKGQEVTENDVTWEIEAYYIAQCAYN
                     TTLMLSPDVIIFGGGVMKQKHLKKKVQDKFIELINGYVAIPDVDNYIMTPKLLDNSGI
                     VGCLALARDVKEKS"
     gene            7848..>8058
                     /locus_tag="IV334_002385"
     CDS             7848..>8058
                     /locus_tag="IV334_002385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transcriptional activator"
                     /protein_id="HAP8944415.1"
                     /translation="MKKKMKDEVLEYIKNNLKYYDFSAQDIAMKFCIKRNVASHYLNQ
                     LFSDGKLLKNDSVRPVMFKYNQQKPK"
BASE COUNT         2769 a         1207 c         1633 g         2449 t
ORIGIN      
        1 ttttttattt gtctcgctta attatacaaa cggcgtgata aaaaacgaga aatgaattat
       61 aggtttagac ctttatcatt tctcgtttta ttttattgta tcaaggctct atcataagtg
      121 gtaaaatcta cttttaaatc acatgtaatc attgataagt ccagtgtttt agcggataat
      181 cagcttttta tttatttttt cttataaatt tgataaatag aaattagtat agccctaaac
      241 aaattatttg tgttttattg tttagggcta tactatattg gtacaaggaa tagaaagagg
      301 tgtttatgtg gttgataaaa ttcagtttaa tcaagttata gaagaattca tcaaagtaaa
      361 taatttaatg acccatattc aaaaacgacc aatcagaata actaatgatc taaaactcag
      421 tacgagtatg attcatttga ttgatgtgat tggaaattat cctgaaacta gtattacgga
      481 attagctgat cgattaggcg ttaccaaagg agctgtatcc caacagattc ccactttaga
      541 gaaaatgaat cttataagca tttctcaaaa aaaaggaaat aagaaaacta aacttgtatc
      601 gttaacaaaa tatggtcgaa aggtgtacga atcacataat tatttacatg aagagcttta
      661 tagctcaata aatgaagcgt tagaatcatt ctcaccagac caaatttcaa ccattaaaca
      721 gattctagaa aaagtatctt atacaatcaa tgaatatcaa gtaagtctga caagaaagga
      781 agattgacat gaaaaaaatg atttttgaat cggctacact agactagaca gaaaaaataa
      841 ggtgtgtaaa ataaatgaaa acacactgga ggaattttta tgtcaagacg tcaacgtaga
      901 acctattcaa aagaattcaa acaacaagtc gtcgatcttt atctcgcagg aaagccccga
      961 gctgaaatca ttcgagatta tgagctgact acctcggcct ttgataagtg ggtcaagcaa
     1021 gcgcaaacta ctggctcgtt tcaggaaaaa gataacttga tccctgaaca agtagaacta
     1081 attgcattac gaaagaaaaa taaacagctt gaaatggaga atgacatttt aaagcaagcg
     1141 gcgctgatat tgggacgaaa agacaagtaa ttgataccaa taagcacaaa tactcaatat
     1201 cagcgatgtg taaagtgtta aacatctctt gccaaaccta ttattatcaa gccaaaccag
     1261 ttgaagatga atcagaactg gaagaactcg ttcaagaaga atttattcgc aatcgaaaag
     1321 cttatggtac caggaagctg aagcagtgct tggccaatct aggtcttcaa gtgagtcaac
     1381 gcagaatcgg ccgtatgatg aaacagcgtg gcttgcgttc aacatacaca ttggctcatt
     1441 tcaaattcca ttcttctact tgtaacgaag ccaagaccgc caatattcta gacggaacct
     1501 tcctacagga aaagtcatta gaagcgattg taaccgacct tacctatgtt cgtgttggga
     1561 agaaatggca gtatatctgc ctgattcttg atttgtttaa tcgagaaatc atcggctatt
     1621 catgcgggga gaagaaagat gcgacgttag tcaaggaagc ttttgcaaag attccgtact
     1681 cgttgaataa tgtacaactt tttcacacgg atcgtggaaa agaattcgat aaccagacga
     1741 ttgacgaaat tttatgggcc tttaatatca cgcgttcctt aagcaaaaaa ggctgcccct
     1801 atgataacgc cgttgcagaa tccacctata aatctgtcaa agtagaattc gttcatcaat
     1861 accgctttga gacattggct aatttgcgat tagagctatt tgattacgtc cattggtgga
     1921 ataccatccg gctgcacggc acgttaggct atgagacacc gatcagtttt cgacagcaga
     1981 gattggcgca gcgaatcctt gataatgagc tcggatgtga taccggtggc gaggcacctt
     2041 aattatttga atgagcttct gccttagaga atcagattcg aggaggctcg ttgtcaaggg
     2101 cagtcggaac accagcagca agcttacgca ataccttata atttttgtca aaaaaagtgt
     2161 tgccattcca catatatctt taaacagcta ttgccagata aaaataaatg atattatata
     2221 tataaatact tatccgaaaa ggggaatgag agagtcaagg ttcttggaat gtcgaatagt
     2281 cgagtaggta gattttctat ctacaacctt gttgtttaaa aatgaaaaaa attggaatta
     2341 gagaagttgc gaaagaggca aatgtttctc ctacgactgt gtcgagggta ttgaatgatc
     2401 gtggatattt gagtgaggaa acaaaaagaa aagtttttga tgccatgaaa aaattagaat
     2461 actatcctaa tgaactggcg agagcacttt ttaaaaacga aacatatacc gtaggtttga
     2521 tttttcctac aattactaat ccatttcacg ctgagttaat tcaagatatc gagttcgagc
     2581 tttccagcag gggatataaa gtgttgctgt gcaatagctt aaacaatcca acaaaagaaa
     2641 aagagtactt gacgatgttg agaaagaatc aggttgatgg aattatagta ggaacgcaca
     2701 ataaagatat tgcggattat gatattccag ggctacctat tgtagcgatt gatagaagtc
     2761 ttggtgaaaa tacagttaca gtatcttgtg ataattacag gggtggggaa ttagctactc
     2821 aactacttgt aaatcatggc tgtcagaata tactgtgtat tcggggagat agtaagataa
     2881 agctacccgc gaatagtagg actttagcat ataaagatgt tttgaataaa ttcatgctgg
     2941 aaccgttagt cctagaagta ccatttgtaa aagaaatcaa tgaaaaaaat gaaataatta
     3001 agacatatct ggaaactcat ccaaatattg acggggtttt tgcaggagat gatttattgg
     3061 cttctttggc gattaacaac ttaggaagtt cgggcaaaag agttccaaaa gaggttaaag
     3121 tgattggatt tgatggagct caacaaacat tgatttataa tccagaatta tcaactattc
     3181 aacagcctat agatcaaata tctaaagtag cagtaaaaaa attattgaat atgattaagg
     3241 gcgaaaagga gacggatgaa ctttcacttc cagtaaagct cataaaaaaa aatacagcat
     3301 aagttaaatc ggcgaaaaat tccttcgtcg atttaaaaat atttttatga aacgggttga
     3361 catatgaaac gggttgacat atattagttg tgaagataaa aaccttttca ctggaggaag
     3421 aaaatggaga aattattaat taaaagcaat aatcatcaac tttactacca acctaaggat
     3481 gtttgggtcg gagatgttat gccttacggg aaagaaggaa aattctttat ttatcatcaa
     3541 cgtgatacta gaaatccagt tccttttgga gaaccgtttg gctgggcttt agcaactacg
     3601 aaagattttg ttcattttga agactttggg gaatctcttt taagagggga tgacaatgag
     3661 agagaccagt ttatctacgc tggttctgta tttgaggcaa atggaaaggt tcacgcattt
     3721 tatacagggt ataatcgtga attcttagcg cagggaaaga catctcaagt gttgcttcat
     3781 gctgagagtg atgattttgt tcattgggaa aaatcagaaa cagcattgga gttagaacca
     3841 caagaagggt atgatattcg aaactggaga gatccatttg tcgtttggga tgatgaaaaa
     3901 gaagaatatt tgctaatttt aggggcgcga aagggtcagg ataaacatct acaaaccgga
     3961 cgtttagtgg aatttacatc aaaagattta actaattggg aattcaaagg tgatttttgg
     4021 gctccgggat tgtataccat gtttgaaatg ccagaacttt tcaagattgg tgactggtgg
     4081 tatcttgtct attcagaata ctcagatgga aataaaatac tctatcgaat gagtcaaagt
     4141 ttggaaggtc cttggattaa gcctaaagat gaagcttttg atggacgggc ttattatgca
     4201 ggtagaacag cttatgatgg aaaacgtcga gtcctatttg gatgggtacc aacaaaagat
     4261 caagaaaatg ataaaaacaa ttatttatgg ggaggtactt tggttcctca tgaagtctat
     4321 caaaaagagg atttttcact tggggtaaaa cccatagacg aagtgtggaa tgcattcata
     4381 aatttacatg atattgccga tgtagagata gaaacagcag acacaagaga agaagtaacg
     4441 cttgtcaaaa atagtggaac tttgtttaat ttagaaacaa caatttcatt tgatgagaat
     4501 cttagtgact tttcactgag attatataaa aaccttgaga cagatgaatc ttacgaatat
     4561 cgcttcaatt tagatgaggg gcgtcttaca tttgataaaa acccttgtta cccttggttt
     4621 caatatatgg ataaaggact tattagacct atatctttaa agcctaacca atcttataaa
     4681 ctaaaagtga ttgttgatga caatataatg acactttata ttgatggaat cgctcttaac
     4741 gctcgtgtac atggttgttt tggtcaagca attactatta cagcaacaaa tggatcaatg
     4801 actgcaataa ataccaagtt ttcaaatgaa ataaagaaat aataaacgtc aatcatgtgg
     4861 caggtctgaa aatcaaacat gacctgtcac atttaaaagg gaggataaat gaatggacta
     4921 caaaaaaatt gccgaagatg tattggaaaa cgttggagga aaagagaata tctcaagcgc
     4981 tacacactgc attactcgtt tacgtttaat tcttaaagac aaaagcaaag cgaatatgga
     5041 aatgcttgag gaaatagaag gtgcaaaagg tgttcttttc aatagtggac agttacaagt
     5101 aattttcgga acaggtataa ttgaaaaggt ttacaaagag ttcgttgaat tgacaggagc
     5161 taaggaagta tccgtttctg aaatgaaaga cgaaggagtc aataattcag gaaatagact
     5221 tcaacgagct tttaaagttt tctcggatat ctttattccg atcattccag cttttattgg
     5281 ggcagcaatg gttttaggaa ttcgttcgtt gattaccact cctggaatgt ttggaatgga
     5341 tggctcattc gctgatcaca atgtattcct aaatgattta tccaaatttc tgggtattat
     5401 tgcaaccaca tttgattatt taccagtttt agttatgtat tctgcaacaa aacgttttgg
     5461 cggaaaccct attttaggat tacttgtagg atttgttatg attcatccag gactagccaa
     5521 tcggaatgat ttcgtcatgg gaactataaa gcctgattat tggcatttat ttggattaag
     5581 cattccagca gttgcgtttc aagggggtgt ttttccagca attttgactt cttggttttt
     5641 agcaaaagtt gagaaaatta caaataaata ttctcctcaa gtacttgctt acattttagt
     5701 cccaactgtg acaattgttc ttgccaactt tgcactattt ttagtgtttg gtccattggg
     5761 gaattttatc ggtaatgttt taggtggaat cattgatttc ctatatatga agatgggtgc
     5821 ctttggtgct tttgtttttg caggcgcgtt acaaccatta gttgtaacag gaacacaaca
     5881 tgctattcaa ggtattgagg caaacttagt cgcaacaact ggattcaatt atatccaacc
     5941 tatttggtca gtttcaatta ttgcccaagg tggcggttgt attggtatgt atttactctt
     6001 taagaaaaaa tctaaagata gagatattgc gatttcaagc tttattccaa cactggtagg
     6061 tattactgaa ccggctatct ttgctgttaa tttgaaatat tcaatcgttc cttttgtttg
     6121 ctcgtttatt ggtgctggtt ttggtggagc atttatgaaa atttttgatg taaaagccat
     6181 tggacaagga ttgactgtat tgccaggtct gactattgct aatccagttc ttccttatat
     6241 atttggtaat ctcatagcat tcattttacc aatcattttt attctcgctt atgataaata
     6301 taaagtgttt atccctgaag ctaaacaccg taaatttgca gaaaaaactg actcaaaaaa
     6361 attgccagtt caagtaaagt gagatgagag catgttaaat ttttttagaa aaaatgaaat
     6421 aagcgttgta gcacctgcaa ccggtgtttt tgaaaaactg gagaaattaa gtgatccagt
     6481 cttttcaaaa ggaatgatgg gacaaggatt tgcaattgat gcaaaaaacg aaacgattgt
     6541 atctccaatc gatggttttg tttcaagtat ttttcctaca aaacatgcct taggaataaa
     6601 aacaaaatca ggaattgaga tacttgttca tgtaggaata gatacagttg agctaaatgg
     6661 tgagggattt gatattaaag ttcaagaagg acaaaaagtt aaggctgggg atattcttat
     6721 gaccattaat tttgtagttt ttgaaaagaa tcacaagact aaagatgtca ttgttgtttt
     6781 tccagactac caagacgaaa ttttcattcc gaaacttgaa gaagtaaaac aaggacaaga
     6841 attgtttgtt cttggctaat gataagtaat taataataga atcgaggaaa atgtatggtt
     6901 tttggggcta ttgaagctgg tggaacaaaa tttgtttgtg ccattggaga taaagatttg
     6961 aacatatttg aaaaagtcag ctttcccacg actacaccca aagaaacaat gaatttagtt
     7021 ttagattttt ttaatcatca taaagatgat ttagaaggta tcggagtggg atcgttcggt
     7081 cctattgata ttcattgtga atctaaaacg tatggcttca ttacatcaac gccaaaactt
     7141 gcttggcagg attttgattt tattggaagt ttaaaaaagc aacttaatat tccaactgct
     7201 tggacgactg atgtaaatgc ctcttgctat ggtgaatatg tttctggaaa tggcagaggc
     7261 ctttcaagtg ttgtttacta taccgtagga acgggcatag gaggtggcgc tatccaaaac
     7321 ggaaacttca ttgaaggttt tagtcatcca gaaatgggac atatgcttgt aaaacgacac
     7381 aataaagaca acttttctgg gacatgtcca ttccacggag actgtttaga gggcttagct
     7441 gctggtccgg caatcgaaag aagatcaggg aaaaagggac aagaagtgac tgaaaatgac
     7501 gttacttggg aaatagaagc ttattacatt gctcagtgtg catataatac aacattgatg
     7561 ttatcacctg atgtcattat atttggtggc ggagtaatga aacaaaaaca cttaaaaaag
     7621 aaagtacaag ataagtttat tgagttgatc aacggatatg tcgctattcc cgatgttgac
     7681 aattatatta tgacaccaaa attgttagat aattctggta ttgtaggttg tttagcttta
     7741 gcaagagacg ttaaagaaaa atcttaattt ggaaaaaaga tcgtctcgac tatttgtttg
     7801 gttgatgcga tctttttttc aattagtaat tgaagggacg cctaaaagtg aaaaaaaaaa
     7861 tgaaagatga agtattagag tatatcaaaa ataatttaaa gtattacgat ttttctgctc
     7921 aagatattgc aatgaaattt tgtattaaaa gaaatgtcgc ttcccactat ttgaatcagt
     7981 tattttcaga tggaaaatta ctaaagaatg attctgttag accagtaatg ttcaagtata
     8041 atcaacaaaa gccaaaag
//
LOCUS       DACDKM010000112         8025 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20834_2016
            SAMN12640370-rid9891663.denovo.112, whole genome shotgun sequence.
ACCESSION   DACDKM010000112 DACDKM010000000
VERSION     DACDKM010000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640370
            Sequence Read Archive: SRR10040891
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8025)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8025)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742835.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742835.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:04:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,012
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 29 of 75
            Pseudo Genes (incomplete)         :: 33 of 75
            Pseudo Genes (internal stop)      :: 19 of 75
            Pseudo Genes (multiple problems)  :: 5 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8025
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640370-rid9891663.denovo.112"
                     /isolate="E20834_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            94..1248
                     /locus_tag="IV334_002386"
     CDS             94..1248
                     /locus_tag="IV334_002386"
                     /EC_number="2.3.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA synthase"
                     /protein_id="HAP8944416.1"
                     /translation="MKIGIDRLSFFIPNLYLDMTELAESRGDDPAKYHIGIGQDQMAV
                     NRANEDIITLGANAASKIVTEKDRELIDMVIVGTESGIDHSKASAVIIHHLLKIQSFA
                     RSFEVKEACYGGTAALHMAKEYVKNHPERKVLVIASDIARYGLASGGEVTQGVGAVAM
                     MITQNPRILSIEDDSVFLTEDIYDFWRPDYSEFPVVDGPLSNSTYIESFQKVWNRHKE
                     LSGRGLEDYQAIAFHIPYTKMGKKALQSVLDQTDEENQERLMARYEESIRYSRRIGNL
                     YTGSLYLGLTSLLENSKSLQPGDRIGLFSYGSGAVSEFFTGYLEENYQEYLFAQSHQE
                     MLDSRTRITVDEYETIFSETLPEHGECAEYTSDVPFSITKIENDIRYYKI"
     gene            complement(1270..2022)
                     /locus_tag="IV334_002387"
     CDS             complement(1270..2022)
                     /locus_tag="IV334_002387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAP8944417.1"
                     /translation="MNEKEWDEFAEEYYEIQQESQVTIAEDVKNYLKTEKVLPAETAA
                     DVAGGSGRFLSLAKEINRYDLIDFSSGMLENAKKEAEKNELPNVSFIKQSLEEFIQSN
                     ETYEMVFTAANPALVHLIQLEKLMKKSSKWCVIIRVVESIDDVFYPIEKQLGMIEEDP
                     NTSPVVMDQFETYLIDEGFKVKKKDFTYFTQESITEELMKLYYDAYAKDPVVTRYIRK
                     LFSEKREILSTTKLTYRLLLWSADKKRTFHDS"
     gene            complement(2386..5052)
                     /locus_tag="IV334_002388"
     CDS             complement(2386..5052)
                     /locus_tag="IV334_002388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HAP8944418.1"
                     /translation="MENKEQAQQAVYKETIDSLMQQTQSSTDGLSTTQAKEKLERDGL
                     NQLKEKPKKPTWKLFLETFKDAMVIVLLIVALIQMVMGDYIEFLVIFAVLMLNSIISV
                     VQTKKAEGSLEALKNLSAPDARVIRDGKEKTIPANELVVGDIVLLDAGDYVPADGRLL
                     DAGSLKVDEGMLTGESTSAEKEVTDITSTVPIGDRTNMVFSGTIVTYGRGKFLVTSTG
                     NNTEIGQVAGLLESTTEQVTPLQRGLDKFSKKLSLAILALSLLILGIQLFRIYLGEGT
                     GDMTADIVSAVMFAVAVAVAAIPEALQSIVTIVLSLGTNKMAKRHAIIRKLPAVETLG
                     STSIICTDKTGTLTQNKMTVVDYFLGNGDTGDFTNDPSKWTADERRLIEISVLANDAS
                     ISEDGTKLGDPTEVAFIDFSEKLNQPYQEIRNNYPRQAELPFDSDRKLMSTGHTIDNK
                     NYLFVKGGPDIVFQRSKKVLLNGEVVPMTDELMKKFQEQNEAFSTRALRVLAFAYRPL
                     DNFDLSFEDEDDLILVGLLAMIDPPRAEVTQAVTEARSAGIKTIMITGDHKTTAVAIA
                     KQIGIFEEGNLALTGTELDELTEDQLNEVLEKVTVYARVSPENKIRIVRAWQNKGHIS
                     AMTGDGVNDAPALKQADIGIAMGTGTDVAKDAAAMVLTDDNFASIVSAVEVGRNVFDN
                     IKKAISYLFAGNLGAIIAIIFALVADWSNPFTALQLLFINLVNDSLPAIALGTEKAEP
                     ATMNRPPRDPNSGIFTDKTLISVTYRGVLIGIAVIFAQWLGMQHSPEFGVAMSFSTLI
                     WCRTLQTFPARSNTQTAIQAGFFSNRSVLLAVVVCSALYCLTMIPGLKEVFSIPSAFG
                     LTEVFTCMGIALVTIVLMEITKLIIVRVQGNK"
     gene            5393..6844
                     /gene="cls"
                     /locus_tag="IV334_002389"
     CDS             5393..6844
                     /gene="cls"
                     /locus_tag="IV334_002389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HAP8944419.1"
                     /translation="MVSSIITALYLLNALIALVAILIKPRDVAAIWAWLLVLFALPGV
                     GFVLYLFFGRGLTDKKKFYLRQSDLKELENFQSFRGDTIEHYDPDMGDKDKQQFVDFF
                     SSLNRMPLTRMNSVTLLTDGQEKLDSLLQDLKKAKHSIHIEYYAFVTDNIGQQVLHVL
                     EEKAAEGVEVRILYDAFGSHGTKAKDFNRLIKNGGHVHTFVTSQRALLRFRLNYHDHR
                     KIVVIDGKISYTGGFNIANQYVNTTKKFGYWRDTHIRIFGAASSLLQLRFLTDWNVSV
                     PEEKKVGYHLNYFFKKADRDESKLADTSIQLVSSGPNNEREQIKLSFIKLITSAKKRV
                     WIQTPYLVPDESVIAALKIATASGVDVKIMIPNKPDHPFIYRATQYYARQLIKENVQI
                     LVYENGFLHAKTLIMDDEICMVGSANQDIRSYRLNFETSAVIYDPEFLEELATQFKED
                     ETHCSSMTTETVKEMSNWLLFKQQISRLFSPIL"
     gene            complement(7173..7988)
                     /gene="thiD"
                     /locus_tag="IV334_002390"
     CDS             complement(7173..7988)
                     /gene="thiD"
                     /locus_tag="IV334_002390"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HAP8944420.1"
                     /translation="MEDQYVQAMTIAGSDSDGSAGMQADMHTFFTRNVYGVSVMTACV
                     AGNSYGIGASVTLPTDFIDKEFELIAKDFQIRAAKTGMLADSKLIETVVKNYQKYDFG
                     PLVVDPVIVTKHGNLLLEESALQSLKEKLVPLAEVLTPNFYEAEKLTGRTLAAEEDFV
                     QAAKELQDMGAKNVMIKGHHAKNAQQETVNDYVLFESGKSFWLNAPFYPTDRINGTGD
                     SLSACIAAELAKGKTVEQAIRLAKDYVNRAIREEINVGHKYGPINHWAAGELE"
BASE COUNT         2324 a         1674 c         1441 g         2586 t
ORIGIN      
        1 aatccttaaa tattctcttt tttatatttc ttttaatctt tttatgatac cttagtaaag
       61 taagtttttt atcataagta aagaggtgtt gaaatgaaaa tagggattga tcgtctttcc
      121 ttttttattc ctaatttata tttagatatg acagagttgg cagaaagccg tggggatgat
      181 cctgcaaaat accatatagg gattggtcaa gaccaaatgg cagtcaatcg tgcaaatgaa
      241 gacatcatta cactaggagc aaatgctgcc agcaaaatcg taacagaaaa agaccgtgag
      301 ctaatcgaca tggtcatcgt cggaacagaa tccgggattg atcattcaaa agcgagtgcg
      361 gtaattatcc accatttact gaaaatccaa tcttttgctc gttcttttga agtaaaagag
      421 gcttgctacg gtggcaccgc agctttgcac atggcgaaag aatatgtcaa aaatcatcca
      481 gaacgaaaag tactagtcat agcaagtgat attgctcgtt acggcttggc aagcggtggt
      541 gaagtgacgc aaggtgtcgg tgctgttgcg atgatgatca ctcaaaaccc gcgtatttta
      601 tcgattgaag acgacagcgt atttctgaca gaagatatct atgatttctg gcgtccagat
      661 tatagcgaat ttcctgttgt tgatggtcct ttatctaatt ctacgtatat cgaatcattc
      721 caaaaagttt ggaatcgaca taaagaattg tcgggtcgag gactcgaaga ttatcaggcg
      781 attgctttcc atattccgta tactaagatg ggaaaaaagg cattgcaaag cgtattagac
      841 caaacagacg aagaaaatca ggaacgtctt atggctcgct atgaagaaag catccgttac
      901 agccgacgaa tcggtaatct ttacactggt tcattatacc tggggctaac ttctctactg
      961 gaaaattcga aatcactaca gccaggagat cgcatcggtc tattcagcta cggttctggt
     1021 gcagtaagtg aattttttac aggttatctg gaagaaaact atcaagaata tctcttcgca
     1081 caatctcatc aagagatgct agattctcga acacggatca ctgttgacga atatgaaacg
     1141 attttcagtg agacgcttcc tgaacacgga gaatgtgcag aatatacttc agatgtacct
     1201 ttttcgatta caaagatcga aaatgatatt cgttactaca aaatataata aaatcataaa
     1261 aagaaccctt cacgagtcgt gaaaggttct ttttttatct gcggaccaaa ggagcagtct
     1321 ataggtcagc ttagtcgtac taaggatttc tcgtttttca ctgaatagtt tgcgaatgta
     1381 cctggtcaca acaggatctt ttgcgtacgc gtcatagtat agcttcatca attcttcagt
     1441 aattgattcc tgtgtaaaat aagtaaaatc ttttttcttc actttgaatc cttcatcaat
     1501 taagtatgtt tcaaattgat ccattacaac tggcgatgta ttaggatctt cttctatcat
     1561 tcctagttgc ttttcgatag gataaaaaac atcatcgatt gattccacta ctctgattat
     1621 cacgcaccat ttgcttgact ttttcatcaa tttttctaat tgaataagat gtaccaatgc
     1681 tggatttgct gctgtaaaaa ccatttcata tgtctcattt gattggataa attcttctaa
     1741 cgactgtttg ataaaggaaa catttggcaa ctcatttttt tctgcttctt tttttgcatt
     1801 ttctagcatt cctgaagaaa aatcgatcaa atcatagcga ttaatttctt ttgctaagga
     1861 aagaaatctg ccagatcctc cagcaacgtc tgctgctgtt tcagcaggca ggactttctc
     1921 cgttttcaga taatttttta catcttcagc aatagttact tgcgattctt gctggatttc
     1981 ataatattct tcagcaaatt catcccattc tttttcattc atttgatttc ctctaatttt
     2041 ttgttatatg ttttcacttt tcggaataaa tcattctcct cttttatcta ttctatctct
     2101 tttgatgaat aataaaatga ttggctttcc gtctatcata aataaaaaaa gggactgtga
     2161 ccaaagtcgt gtcaaagtcc ctctttccga ataaacagtg tccaatcaga tgaccctcag
     2221 tattagctca aaatgctgat tttacaactt cttccttaaa cggtgttctc ctgccaactc
     2281 ttcggtcaca agtcacaata ttcaacaaac atgagaagct gtttgttgaa attgcacctt
     2341 gtgactcaga attaaacggc aggctcacaa cctcgttgat tcgttttatt tatttccttg
     2401 tactcgtaca atgatcaatt tagtgatttc catcaaaaca attgtcacaa gtgcgattcc
     2461 catacatgta aagacttctg tcaatccaaa tgctgatggg atagagaaga cttctttcag
     2521 tcctgggatc attgttaagc agtaaagcgc gctacataca actacagcta gtaagacact
     2581 tctgttagag aagaaccctg cttggatagc tgtttgtgtg tttgaacgtg ctgggaatgt
     2641 ttgcaatgtc cgacaccaaa tcaatgttga aaatgacatg gctacaccga attcaggtga
     2701 atgctgcatg ccaagccatt gagcaaagat aacagcaatc ccaatcagca caccacgata
     2761 cgttacagaa atcaatgttt tgtccgtaaa gattcctgaa tttggatctc ttggcgggcg
     2821 gttcatcgta gcaggttcag ctttttctgt acctaatgca attgctggca gtgaatcatt
     2881 gactaaattg atgaacaaca gttgtaatgc tgtaaatgga ttggaccagt cagcaactaa
     2941 ggcaaagatg atcgcaatga tcgcacctaa atttcctgca aatagataag aaatagcttt
     3001 cttgatatta tcaaacacat tacgtcctac ttctactgcg ctgacaatag aagcaaagtt
     3061 atcatccgtc aataccatcg ctgctgcatc ttttgccaca tctgtacccg ttcccatagc
     3121 aattccgatg tctgcttgtt tcaatgctgg tgcgtcattt acaccatcac ctgtcatagc
     3181 tgagatatga cctttatttt gccaagcacg aacgatacga attttattct caggtgaaac
     3241 acgtgcataa acagtgactt tttctaaaac ttcgtttagt tgatcttctg tcaattcatc
     3301 tagttccgtc ccggtcaaag ccaggtttcc ttcttcaaaa atcccgattt gttttgcgat
     3361 agcaacagct gttgttttgt ggtcaccagt aatcatgatt gttttgattc ctgcacttct
     3421 tgcttctgtt accgcctgag tcacttcagc acgtggagga tcgatcatag caagcaaacc
     3481 aacaaggatc aaatcgtctt catcttcaaa tgataaatca aaattatcaa gtggacggta
     3541 agcaaaagct agtacacgta atgcgcgtgt tgaaaaagct tcattttgct cttggaattt
     3601 tttcatcaat tcatccgtca tcggaacgac ttcaccattt agcaatactt ttttactgcg
     3661 ttggaagacg atatctggtc cgcctttaac aaacaagtaa tttttgttat caattgtgtg
     3721 tccagtagac attaattttc gatctgagtc aaaaggaagt tctgcttgtc ttgggtagtt
     3781 gtttcggatt tcctgataag gctggtttaa tttttcacta aagtcgataa acgctacttc
     3841 cgtagggtcg cccaatttag taccgtcttc actgatcgat gcatcattcg ctaaaacact
     3901 gatttcgatc aagcgacgtt cgtcagctgt ccattttgaa gggtcatttg tgaaatcccc
     3961 agtatcgcca ttacctaaaa agtagtcaac aactgtcatt ttgttttgtg tcaatgtacc
     4021 agtcttatct gtacagatga tacttgtcga accaagtgtt tcaactgctg gaagcttccg
     4081 aataatggca tgacgttttg ccattttatt cgttcccaaa gataacacga ttgttacgat
     4141 agactgtaat gcttctggaa tagcagctac ggctacggct acggcaaaca tcactgcgct
     4201 gacgatatct gcagtcatat ctcctgtacc ttctccaaga taaatacgaa acagttggat
     4261 acctaaaatc aataaagaca atgccaaaat agccaaactt agttttttac tgaatttatc
     4321 cagacctcgt tgtaacggag tgacttgctc agttgttgat tccaaaaggc cagcaacttg
     4381 tccgatctcc gtattattcc cagttgaagt cactaagaat ttaccgcgtc cgtaagttac
     4441 gatcgttcca ctgaatacca tatttgtacg atctccgatt ggtaccgtac ttgtgatatc
     4501 cgttacttct ttttctgcag aagtcgactc tcctgtcagc ataccttcat ctacttttaa
     4561 tgatcccgca tctagtaaac gtccatcagc aggtacataa tcgcctgcgt ctaacaacac
     4621 gatatctcct accacaagtt cgtttgccgg aatcgttttt tccttaccat cacgaatcac
     4681 tcgagcatcg ggcgctgaaa ggtttttcaa cgcttctaaa gaaccttctg ctttttttgt
     4741 ttggacaaca ctgattatag aattcaacat caatactgca aaaataacta agaattcgat
     4801 gtagtcaccc atgaccatct gaatcagtgc gacaatcagc aatacgataa ccattgcatc
     4861 tttgaaagtt tctaagaaca atttccaagt tggcttcttc ggcttttctt ttagctggtt
     4921 caagccatct ctttctaatt tttccttcgc ttgcgtggtg ctcaatccat cggttgagct
     4981 ctgcgtttgc tgcatcaacg agtcgatcgt ttctttatag accgcttgtt gtgcttgttc
     5041 cttgttttcc aaatttcatc ccccattttt tgatcttggc cctagtcctg tctgaaaaaa
     5101 aagagaccta agacaaatac gcatttgtct taggtctcac catttcatac aataccagaa
     5161 aaccatttgg ttaacttact gttgatatta tcgcaacaaa cgttgccggt tactccccta
     5221 aggagatatt caactgtact aatatcctac cttattaacc tacaaaaagc aactacttta
     5281 gcccgattta cctacatttt catgaaagga gtgaaaagta tattcacctt ggtaggcttc
     5341 tttcttctat catgctatac taatgttatt gtatgtaagg aggaacattt ttgtggtatc
     5401 tagtattata accgcccttt atctattaaa tgcacttatt gctttggtgg ctattttgat
     5461 caaaccccga gatgtagcag ccatttgggc atggctctta gtattgtttg cccttcctgg
     5521 ggtgggcttt gtattatatt tatttttcgg acgtggatta acggataaga aaaaattcta
     5581 tctccgacaa agtgacttga aagaattaga aaactttcag tcttttagag gagataccat
     5641 tgaacattac gatcctgaca tgggcgataa agacaaacag caatttgttg acttcttctc
     5701 ttcattaaat cgtatgccgc tgacaagaat gaattctgtc actcttctca cagacggaca
     5761 agagaaattg gattcactgc ttcaagatct aaaaaaagcc aaacattcga tccatatcga
     5821 atattacgca tttgtgacag ataatatcgg ccagcaagtc ttacatgttt tagaagaaaa
     5881 agccgcagaa ggcgtggaag ttcgaatatt atatgatgca tttggctctc atggcacaaa
     5941 agcaaaagat ttcaatcgtc taatcaaaaa tggtggacat gtccatacat ttgttacctc
     6001 acaaagggca ttacttcgtt tccgattgaa ttaccatgat caccgaaaaa tcgttgtgat
     6061 cgatggaaag attagttata ccggtggttt caatattgcc aatcaatatg taaatacaac
     6121 aaaaaaattc ggctattggc gcgatacgca tatacggatt ttcggtgccg cttcttcttt
     6181 gctccagctt cgcttcttaa cagactggaa cgtctcggta cctgaagaaa aaaaggtcgg
     6241 ctatcatttg aattatttct ttaaaaaagc agatcgagat gaatctaagc ttgctgatac
     6301 atccatccag cttgtttcaa gcggaccgaa taacgaaagg gaacaaatca agctttcatt
     6361 tatcaaattg attacttctg ctaaaaaacg tgtttggata cagacacctt accttgttcc
     6421 tgatgaaagt gtcattgctg ctttaaaaat cgcaactgcc tctggtgtag atgtgaaaat
     6481 catgattccc aacaaaccgg atcatccttt tatttatcga gcaacacaat attatgctcg
     6541 gcagctgatc aaggaaaatg tacaaatcct tgtctatgag aacggcttcc tccatgcaaa
     6601 aacattgata atggatgatg aaatctgcat ggtaggttca gcaaatcaag atattcgtag
     6661 ctaccgattg aattttgaaa caagtgctgt catttacgat cctgagtttt tagaagaact
     6721 tgctactcag ttcaaagaag atgagacaca ttgttcatcc atgacaactg aaacagtcaa
     6781 ggaaatgtct aactggctat tattcaagca acaaatttct cgattatttt ctccaatcct
     6841 ataatttttt tattcaagta gagaaaaata tactaagatt aaagggactg tgacaaaaat
     6901 cttgtcacag tcccttcttc cgaacaaacg gtgttctcct gccaactcct cggtcacaag
     6961 tcacaatatt caacaaacat gagaagctgt tttttgaact tgttccttgt gactcggagc
     7021 tggacggcag gctcacgacc tctttcttaa acggtgttca aaagcttgca tctgcggtaa
     7081 taagtccaac caagttaaaa acataagaag ctgtttttac ttggctgttc ttattacttg
     7141 atgcagggca actttttcac aacttctttc ttttactcta attcaccagc tgcccaatga
     7201 ttgatggggc catatttgtg ccctacattt atttcttcac gaattgcacg gtttacatag
     7261 tcttttgcca atcggatcgc ttgttcaact gtttttcctt tcgctaactc agctgcaata
     7321 catgcagata gcgaatcgcc agttccgttg atccggtcgg taggataaaa tggtgcattt
     7381 aaccaaaatg attttcctga ttcgaacaat acgtagtcat tgactgtttc ttgctgcgca
     7441 tttttagcat gatgtccttt gatcataacg ttttttgcac ccatatcttg cagttctttt
     7501 gctgcttgca caaaatcttc ttcagctgct aatgtccgtc cagtcagctt ttctgcttca
     7561 taaaaattag gtgtcagtac ttcggccaaa gggacaagct tttctttcaa gctttgcaaa
     7621 gcagattctt ctagtaaaag atttccatgt ttcgttacga ttacgggatc cacgactaat
     7681 ggtccaaaat catatttttg gtaatttttt acaactgtct caatcaactt tgaatctgct
     7741 agcatacctg ttttagctgc acgtatttga aagtccttcg caatcagttc aaattctttg
     7801 tcgataaaat ctgttggcaa tgtcacactt gctccgattc cgtaagaatt acctgcaaca
     7861 catgcggtca tgacagaaac accgtaaaca tttcttgtaa agaatgtatg catatctgct
     7921 tgcatccctg cgcttccatc gctatctgat cctgcgatcg tcattgcttg tacatactga
     7981 tcttccatca aatcgattcc tccattttta taaaaagatt tttca
//
LOCUS       DACDKM010000113         7745 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20834_2016
            SAMN12640370-rid9891663.denovo.113, whole genome shotgun sequence.
ACCESSION   DACDKM010000113 DACDKM010000000
VERSION     DACDKM010000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640370
            Sequence Read Archive: SRR10040891
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7745)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7745)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742835.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742835.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:04:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,012
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 29 of 75
            Pseudo Genes (incomplete)         :: 33 of 75
            Pseudo Genes (internal stop)      :: 19 of 75
            Pseudo Genes (multiple problems)  :: 5 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7745
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640370-rid9891663.denovo.113"
                     /isolate="E20834_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(<1..152)
                     /locus_tag="IV334_002391"
     CDS             complement(<1..152)
                     /locus_tag="IV334_002391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
                     /protein_id="HAP8944421.1"
                     /translation="MNHFKGKQFKKDVIIVAVGYYLRYNLSYREVQELLYDRGINVCH
                     TTIYRWV"
     gene            228..1697
                     /locus_tag="IV334_002392"
                     /pseudo
     CDS             228..1697
                     /locus_tag="IV334_002392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304880.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="family 20 glycosylhydrolase"
     gene            1825..4806
                     /locus_tag="IV334_002393"
     CDS             1825..4806
                     /locus_tag="IV334_002393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 42"
                     /protein_id="HAP8944422.1"
                     /translation="MLSDYYQNLSVISVNTEPLRNYYIPYASKEEAKQELEREKTSQF
                     IDLNGTWDFQYYPSVQQLPENFFQEAFTESYDRISVPGVWQTEGFDLPQYINVSYPIP
                     FDPPYVPNDTPCGVYRRQFQIEQTDARFYLNLEGADSNCFVWINRNFVGYDQVSHSTS
                     EFDVTKYLQVGVNEIQILVMKWCDGTYFEDQDKWRMSGLFRPVYLLQRPTMHLTSYQL
                     RTLTDPKKGSGTLMVSFQRFTPIEVSLPISYELFHPDGRLIAKGECYSEQQINLDVED
                     CLFWTAETPKLYTLLLTCNGEYFKEKVGFRALTIKNRKLLLNGTPIKLYGVNYHDVNQ
                     QGTPVMTREQLRADLLLMKKLNVNTVRTAHYPKSPDFYELADEIGLYVINEADLECHG
                     VVNLYDTEANYQLIANDPTFEKPIMDRVIRSILPYQNRSSIIMWSMGNESGYGVNFEK
                     AQALARKLDDSRLIHNERSIEPVIDQVNDFSNLDVISQMYPPLEDVHRHCQRSDTEKP
                     FFMCEYSHAMGNGPGDLKTYFDLIQQYDSFLGGCVWEWTDHAINRGTLETPSYLYGGD
                     FGEKYHDGNFCVDGLLTPDRQLTVGALEYRNIHSPIQLIAVDEELQEVTLSNITAFTA
                     GSAFNLSLEWYIDGKKTVYEVMNPFILEPGKSCSLPITVPTTIHGAHTVAVSLSVYKN
                     DELYGQQQLFLKKEKMRLPRTAHHNDAPQISVKGTELIIKGESFIYIYQLAKAAFTSV
                     KINQKELLERPTQFSIWRAPTDNDRNLVNKWKAAGYAEMSLEIRKKDWKFVDDHLLIE
                     TSYRGVIPGLQPIFTMHIQLKINGAGVINQKVSVEKNPIFPDFPRFGMDWPLNDTFSK
                     VSYCGYGPYESYPDKHYAAMFGIYEMEIEEFDQPYIKPQEYGNRWQCDWVSIKNHQGS
                     TMTVYGNSNFSALPYSQAQLTEKSHHYELIKENRTWFNLSYKQNGIGSHSCGPYLEDR
                     FRFMEDTFDWNIVITFKGENR"
     gene            4803..6464
                     /locus_tag="IV334_002394"
     CDS             4803..6464
                     /locus_tag="IV334_002394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hyaluronate lyase"
                     /protein_id="HAP8944423.1"
                     /translation="MKETKINQVKKKLSRYQEVVYRNHCLKWSKNKLNVRFNGLFYRF
                     EQLEEVWKPVTNFQSCPVQLINDPKETYDLCITHLYNQHLKNDGFYLKVTNDNQLTIE
                     SANLRGFQYAMEALLESFFSVGDQLLLPELILKHEPSVKIRGIIEGFYGIPWTHEMRL
                     SLFSYMKDNQLNTFMYAPKDDELLRKKWRELYDAQELDKFKELLSAAEASNIDFWYLI
                     SPGNDIDITCEEDIQVLLKKLEQLIELGVFQFGLLMDDIDYQLKGAAKRRFREPAFAH
                     AYLVNRVEEYLSTRLSNHELVICPTEYDNRHGSRYLATLSQEIPEQLPFFWTGPSTLA
                     ASISTEELQEMASVYQRPMLIWDNIPVNDYLEDKELLFMSPYENRTPNLSKERYQVTG
                     VVSNPMAQLEASKFTINSMANYLWNCERFDPLETWTSVVEKVVGSKLQPAYLTLTNAF
                     PNHYTSSLLSDEELLLAKDPEWVTKEMAALVQAVKWIQQEQHTSRLRTELEPWLQAIT
                     ESWTFWQEWQLKKDPKEAEEWLAKKKTHRIGRDLVTAHLEQIIKS"
     gene            6504..7436
                     /locus_tag="IV334_002395"
     CDS             6504..7436
                     /locus_tag="IV334_002395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="haloacid dehalogenase-like hydrolase"
                     /protein_id="HAP8944424.1"
                     /translation="MVKRLISANYSDVKKMTSKELKQSIKASEGRVIVCETVANTLPQ
                     SGEITNAEVASAFGADLILLNGFDCYRPAVFGLPNVTIEKVFDLQQTIENPIPKLKEL
                     VGRPVGINLEPIAPKTNMMSEKVEISQGRASSVETIQKAEELGVDFICFTGNPGTGVT
                     NLEITKSVKMAKKYFSGLIIAGKMHSAGSEEPVVNKEAIISYAEAGADILLVPSVGSI
                     PGFLEQDLIEAVRLAKKYDLLTMSAIGTSQESARPETLRQLAIMNKSCGVDLQHIGDA
                     GYGGLAPAENIFETSVAIRGMRHTINCVSRSINR"
BASE COUNT         2578 a         1281 c         1585 g         2301 t
ORIGIN      
        1 acccaacgat aaatcgtagt atgacaaaca tttattccac gatcatataa caattcctga
       61 acttcacgat agcttagatt gtaacgcagg tagtaaccaa cagcgacaat aatgacgtct
      121 tttttgaatt gtttgccttt aaaatgattc attactctgt cctctctgtc ttttttctca
      181 attttacact aaaatagatt ttttggaaaa ctttgcaaca gaacccaaaa gaagaagtga
      241 atgaaattgt tgaatttgct tctggttttg gcatagaagt agttccatcg atccagaccc
      301 ttgctcactt gacccaattt ttgaagtggt atgctcaaca agaactgatg gatgatcaaa
      361 atactctact gatcggtgaa gaaaaggttt atgcggcaat cgaaaagatg attattgcct
      421 gcaaagaaat gtatcagaca gagcgtattc atttgggaat ggatgaagcc tatcaagcag
      481 gtctgggacg atatttaagc ctgtatgggt attgtgatcg gacaacttta atgattcaac
      541 atatggaccg tgtgttatca attgttgata aatataagat gaaaccaatg atttggagtg
      601 atttcattta taagctatta gatgaaaccg gtaaagatag gtattacaat cctgatttta
      661 aactcaaagc attagcagct aaaggattac cagcaggatt aacctatgtt cattgggact
      721 atggttcaga tgaagtttcc tcctatgaga aagtaattga taaccatttg aaattttgtg
      781 acattgatca ttacgtttta gctagtggtg cccatatctg ggggagacta gccccgaatc
      841 atgggaaaac agaagcaatt gtttctgcag ctgtaaaagc ttctaaaaat aaaggtgtac
      901 gttctgtgat gttaactact tggggagatg aagggcagga aacagagcat tggcattcct
      961 taattggggc attaaaatta gcagattatg tctattctga agaagagtgg aatgattttg
     1021 aggaaatcta cgatcgttta ttaggaacag gtagttatca attaatgtcc gaattacgat
     1081 atttcgatga agtggatacc attacaaata acaacttaca aatggcttcc gtgagtaaac
     1141 tgctactttg gcaagatccg ttagttggta tatatgactt tcatgtagcc gctcataaca
     1201 aaattgccac tagaacaatt ggtacttatt atcatgagtt agctcagaaa ttagatatga
     1261 ataaaaattg gaatccaaaa caaccaattt taacaactat tcgtcaacga tatatacttt
     1321 tagctgaaat tttagcacaa aaggcagaac taggcatcta tttacaacac tgctgtctgg
     1381 tagaagacct tgcaggatta atggaattag cgaatcggtg tgaagcacta gccaatcgag
     1441 taaatgaatt ggcatctatt catgaaataa tttggtatca aacatacaaa acctatggtt
     1501 gggaagtatt agaacaacga tatgctgcat tagctcatcg tctgaaaacc gttgcgcgtc
     1561 gtattaaaaa tcatcttcag gggcaagatg ccctagagga gcttttggaa gaacgattac
     1621 cgttttgtca atatcaagtg ccgcttgaaa tttctggctt taattatcgg caaagctcgg
     1681 tgtcaggcta caattaacga aggaatcttc ccgttgaacg gaccacttgc tcatctaatg
     1741 ggaaagtggt tcgttttcta ttcataagta gcttaccact aactctttta ttaagaagag
     1801 aaaaaaagta ggaagtgaga aaatatgtta tcggactatt atcaaaatct gtcagttata
     1861 agtgtcaata cagaaccatt acgtaattat tatattcctt atgcaagtaa agaagaagca
     1921 aaacaagagt tagaaaggga aaaaacgagc cagtttatcg atctcaatgg tacatgggat
     1981 tttcagtatt atccttctgt ccaacagtta ccggaaaatt tttttcaaga ggcctttact
     2041 gaaagctatg atagaatttc tgttcctgga gtttggcaaa cagagggatt tgacttacct
     2101 cagtatatca atgtatctta tccaattcct tttgatccgc cttatgtacc taatgatacc
     2161 ccttgtggtg tatatcggcg tcagtttcaa attgagcaaa cagatgcacg cttttactta
     2221 aatctagaag gagcagatag taattgtttt gtttggataa atagaaactt tgtaggctat
     2281 gaccaagtga gtcatagtac atctgaattt gatgtgacca agtatttaca ggttggggtt
     2341 aatgaaattc aaattctagt gatgaagtgg tgtgatggta cctactttga agatcaggat
     2401 aagtggcgaa tgagcggtct atttcgtcca gtgtatcttc ttcaacgacc aactatgcat
     2461 ctgacaagtt atcaactacg gacgttgact gatcctaaaa aaggaagcgg aacattaatg
     2521 gtttcttttc aacgatttac tcctattgaa gtatcgttac ctatttctta cgaattattc
     2581 catcctgatg gacgtctcat agcgaaaggg gagtgttact cggaacaaca aataaatttg
     2641 gatgttgaag attgcctttt ttggacagca gaaacgccta aattatatac attactttta
     2701 acatgtaatg gtgaatattt taaagaaaaa gttggctttc gagcactgac gatcaagaat
     2761 cgtaagttat tgctgaacgg aacacctatt aaattatatg gagtcaacta tcatgacgtg
     2821 aatcagcaag gcacgccagt gatgacccga gagcagttac gtgcagattt gttattaatg
     2881 aaaaaattaa atgtgaatac tgtacgaacg gcacattacc ctaaatcacc agatttttat
     2941 gaattggcgg atgagattgg tctgtatgta atcaatgaag cagatttgga gtgtcatggt
     3001 gtggttaatc tatatgatac tgaagcgaat tatcaactaa ttgctaatga tccgacattc
     3061 gaaaagccga ttatggaccg agtgatccgt tctattcttc cttatcaaaa tcgaagctct
     3121 atcattatgt ggtctatggg gaatgaatct ggttatggag taaattttga aaaagctcaa
     3181 gctttagcac ggaaattgga tgattcacgg ttgattcata atgaacgctc gattgaacca
     3241 gttattgacc aagtgaatga ttttagtaac ttggatgtta ttagtcaaat gtatccccca
     3301 ctggaagatg ttcatcgtca ttgtcaaaga agtgatactg aaaaaccttt ttttatgtgt
     3361 gaatatagcc acgctatggg caacggtcct ggagatctta aaacgtactt cgatttgatt
     3421 caacagtatg attcctttct tggcggttgt gtctgggagt ggactgatca tgcaatcaat
     3481 agaggaactt tagaaacgcc atcttattta tacggtggtg attttggtga aaaatatcac
     3541 gacggcaatt tttgcgtaga tggtttattg accccagatc ggcaattaac agtaggtgca
     3601 ttggagtacc gtaatattca ttcgcctatt caactgattg ctgtagatga ggagctgcaa
     3661 gaagtaacct tgagcaatat aactgctttt acagccggtt cagcatttaa cctttctctt
     3721 gaatggtata tagatggaaa aaaaacagtt tatgaagtaa tgaatccttt catacttgag
     3781 cctggcaaaa gttgttcatt gcccatcaca gtacctacaa cgattcatgg tgctcatacg
     3841 gttgctgttt cgctatctgt ttataaaaat gatgagcttt atggacagca acagctcttt
     3901 cttaaaaaag aaaaaatgag attgccaaga actgctcatc ataatgatgc accacaaatt
     3961 tcagtaaaag gcacagagct gataatcaaa ggagaatcct ttatttatat atatcaatta
     4021 gcaaaagctg cgttcacttc tgtaaagatc aatcaaaagg aacttttgga acgtcctact
     4081 caattttcaa tttggcgtgc accaacggat aatgatcgta acttagtaaa caaatggaaa
     4141 gcagctggat atgctgaaat gtctttagaa atcagaaaaa aagactggaa attcgtagat
     4201 gatcatctcc tgatcgaaac gtcttatcga ggagttattc caggattaca gccaattttt
     4261 acaatgcaca tacagttgaa aataaatggt gcaggcgtaa tcaaccaaaa agtatcagtg
     4321 gagaaaaatc cgatttttcc agatttccct cgctttggga tggactggcc gttgaatgat
     4381 actttttcga aggtttctta ttgtggctat gggccgtatg aaagttatcc tgataagcac
     4441 tatgctgcga tgtttggtat ttatgagatg gagattgagg aatttgatca gccatatatc
     4501 aaaccacaag aatatggtaa ccgctggcaa tgtgattggg tatcaatcaa aaatcatcaa
     4561 ggatctacca tgacggttta tggaaactct aatttttctg ctctgcctta tagtcaagct
     4621 caattaaccg aaaagtcaca ccattatgaa ttaataaaag aaaatagaac ttggttcaat
     4681 ttaagttata aacaaaatgg tatcggctca cacagttgcg gtccttattt agaagatcgt
     4741 ttccgtttta tggaagatac ttttgactgg aatatagtga ttactttcaa aggagaaaat
     4801 agatgaaaga aacaaagatc aatcaagtca aaaaaaaatt atctcgttat caagaagttg
     4861 tttatcgaaa ccattgctta aaatggtcaa aaaataagct gaatgttcgt tttaatggat
     4921 tattttatcg tttcgaacag ttagaagaag tctggaaacc ggtaacgaat ttccaatcat
     4981 gtcctgtaca gctcataaat gatccaaaag aaacatacga tttatgtata acacaccttt
     5041 ataaccagca tttgaaaaat gacggatttt atttaaaggt tactaatgac aatcaactta
     5101 cgattgaatc agcaaacctt cgagggtttc aatatgctat ggaagcctta ttagagagtt
     5161 ttttttcggt aggagatcaa ttattattac cggaattgat tctgaaacat gaaccatcag
     5221 tgaaaattcg aggaatcatt gaagggtttt atggtatccc ctggacacat gaaatgcgcc
     5281 tctctctttt ttcatatatg aaagataacc aattgaatac ttttatgtat gcccctaaag
     5341 atgatgaatt attacgtaaa aaatggcgtg aactctacga tgcgcaagaa ttagacaaat
     5401 ttaaggagct tttatctgct gctgaggcta gcaatatcga tttttggtac ttaattagtc
     5461 ctggaaatga tattgatatt acgtgtgaag aagacattca agttcttctg aaaaagttag
     5521 aacaactgat tgagcttggc gtatttcaat ttggtctatt aatggatgat attgactatc
     5581 aattaaaagg tgctgctaaa cgccgttttc gagagccagc attcgctcat gcctatttag
     5641 tgaatcgagt agaggaatat cttagcactc ggttgtccaa tcatgaatta gtcatttgtc
     5701 ctacagaata tgataatcgt catggttctc gctatttagc aacgttaagt caagaaatac
     5761 cagaacaatt accgttcttt tggactggcc caagcacgtt agcggcgtca atttctacag
     5821 aagagctgca ggaaatggct tctgtctatc aacggccgat gctgatttgg gataatattc
     5881 cggtaaatga ttatttagag gataaagaac tattgttcat gagtccatat gagaatcgaa
     5941 caccgaatct ttctaaggag agataccagg ttacaggagt agtttcgaat ccaatggctc
     6001 aattagaagc ttcaaagttt acgatcaata gtatggctaa ttacttatgg aactgtgagc
     6061 gttttgatcc tttggaaact tggacgtcag tcgttgaaaa agtagttgga agtaaactac
     6121 aaccagctta tcttacctta accaatgctt ttccaaatca ttacacaagc agtctgcttt
     6181 cagatgaaga attactgcta gccaaagacc cagaatgggt cacaaaagaa atggctgctt
     6241 tagtccaagc agtaaaatgg atccagcaag aacaacatac atctagattg agaacagaat
     6301 tggagccttg gttacaagca atcacagaaa gttggacttt ctggcaagaa tggcaattaa
     6361 aaaaagatcc aaaagaagca gaagaatggt tggctaagaa aaaaacacac cgcataggac
     6421 gtgatttagt aactgcccat ttagagcaaa ttatcaaaag ttagatatct taaaaaataa
     6481 aaagcaagaa aaaggagaac aaaatggtca agagattaat tagtgcaaac tacagtgatg
     6541 tcaagaaaat gacgtcaaaa gagttgaagc aatccatcaa agcgagtgaa ggacgggtga
     6601 ttgtttgtga aacggttgct aatactcttc cacaatctgg agaaattacg aatgcagaag
     6661 ttgcgtcagc atttggtgca gatttgattc ttttaaatgg tttcgattgt tatcgtcctg
     6721 cagtttttgg actaccaaat gtgacgattg agaaggtttt tgatctacag caaacgattg
     6781 aaaacccgat tcctaaatta aaagaactag tcggtcgtcc tgtgggaatt aatttagaac
     6841 ccattgcccc taaaacaaat atgatgagtg agaaagtaga aatcagccaa ggaagagcaa
     6901 gctctgttga aacgatacaa aaagcagaag aactaggagt cgattttatt tgctttaccg
     6961 gtaatccagg cactggagta accaatttag aaattactaa atccgtaaaa atggcaaaaa
     7021 agtatttttc gggattgatt attgctggga aaatgcatag cgccggctca gaagagccag
     7081 ttgtgaataa agaggcgatt atttcttatg cggaagcagg cgcagatatt ttactagttc
     7141 cttcagtagg gtctatccca ggctttttag agcaagacct aattgaagcg gttcggttgg
     7201 caaaaaaata tgatttgtta acgatgtctg caatcggtac tagtcaggaa agtgcccgac
     7261 cagaaacatt gcggcaatta gccattatga ataaaagttg tggtgttgat ttacaacata
     7321 ttggagatgc tggatacggg ggattggcac cagcagaaaa tattttcgaa acctcggttg
     7381 caattagagg aatgcgacat accatcaact gcgttagccg ctcgatcaat cgctaacaaa
     7441 attaaataaa aaatggttaa attgattatt taagtgggaa catacttgag atatcagcta
     7501 acttctgtca ctttaaaaaa gggactgtga cagaagtgaa aatagacaaa aaatatccga
     7561 acaagagact aggatagcag aaaaactatc gttttgcaac gaggaccaac tggaccgtag
     7621 gagcaattct cttattgttc ggatatttcg cttgttcaag gacttatgtc tgtagtgccc
     7681 ttttttggta ggaaaaatgg tagggaacca tatcattttt aatgaaaatc aagcatttca
     7741 aaagt
//
LOCUS       DACDKM010000114         7627 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20834_2016
            SAMN12640370-rid9891663.denovo.114, whole genome shotgun sequence.
ACCESSION   DACDKM010000114 DACDKM010000000
VERSION     DACDKM010000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640370
            Sequence Read Archive: SRR10040891
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7627)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7627)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742835.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742835.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:04:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,012
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 29 of 75
            Pseudo Genes (incomplete)         :: 33 of 75
            Pseudo Genes (internal stop)      :: 19 of 75
            Pseudo Genes (multiple problems)  :: 5 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7627
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640370-rid9891663.denovo.114"
                     /isolate="E20834_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            304..660
                     /locus_tag="IV334_002396"
     CDS             304..660
                     /locus_tag="IV334_002396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HAP8944425.1"
                     /translation="MKLEVTKEAQEVLKNKLEADDQLLLDIENGDGPFAESQVSCQLD
                     TAFRLIIVKKDTQTDLSLYSVVADTPVGPIKIKDSAILYMDDPSTLALEPTYNSLQLK
                     GPSGLRKGNLQVVRKD"
     gene            670..1569
                     /locus_tag="IV334_002397"
     CDS             670..1569
                     /locus_tag="IV334_002397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohydrolase"
                     /protein_id="HAP8944426.1"
                     /translation="MGKLAIISDLHVDINKFGESELMQLWQVLQKHKITRLHLAGDTA
                     NKVDRCLAVVEFFVNKGLSTTFHFGNHELADVPNEQMMDHFPDPHFLNERYIALNEQT
                     VLLGMNGWYDYQFSELKDYEAIVRMKRLYWYDRFIQRGKTDLEVNAEMLAAAKNLLDQ
                     LEAKKLEVILSTHFVPKRAFIMYQSAPYERWNKLNAFLGSESFGELLDRYSNIKQVVF
                     GHTHRRFEDKSIGHTIYSCRPFGYYYEWQLTRDFVLKEQLLDSYDPMKLRVLLRAHHP
                     AFQAYQTQHLQEEFEQAMTIVPY"
     gene            complement(1966..3045)
                     /locus_tag="IV334_002398"
     CDS             complement(1966..3045)
                     /locus_tag="IV334_002398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006908283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HAP8944427.1"
                     /translation="MTQNHDNTVSRKGKHLTYSERCQIAILKKENYSNRQVADVLGCV
                     PQTINNEIKRGTVTQLNRQNQNGKVYDYYHDVYDADAGQAAYEKNRLNCGRRPLWADT
                     DAFIEWADDMMLLEKWSPDVVVGFARKHELFDPAIIPGTTTLYQWIDRGIMKTKNMDL
                     LEKLSRKPKSSRHGNRPNKRVLGKSIEERPEAVGTRKTFGHWEVDTVVGNKTKSDAVL
                     LTLVERQTRFEVILKLAGKDAESVNQAVRQLRERAGAYFGQLFKTVTSDNGSEFAGLH
                     DALQDVANIYFSHPYASWERGTSENQHKLIRRFIPKGSPIGNVSENQILRIQRWMNDY
                     PRKILGYATPHDTFVQAFRQERLTA"
     gene            3475..4206
                     /locus_tag="IV334_002399"
     CDS             3475..4206
                     /locus_tag="IV334_002399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HAP8944428.1"
                     /translation="MMKKEIVLTAAMMLFSMVASTTFVSATEVYPKEYNTEGTITFEA
                     GDEGVTPPVDPENPDPNKPVDPSDPPSPGTGGALSIDYGSKFKFGTQKISTADKTYYA
                     AADVMNDGSRKPTYVQVTDRRSTLSGWKLSVSQPEQFKTASGDELVGAQLKFTKGQAV
                     SLVDPTYTPQTVNSELTLTPGGNNTLAINAKSGTGVGTWVYRFGANENENQDAVQLSV
                     PGKSVKLAQQYSTKLVWTLEDTPNN"
     gene            4351..6378
                     /locus_tag="IV334_002400"
     CDS             4351..6378
                     /locus_tag="IV334_002400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lectin"
                     /protein_id="HAP8944429.1"
                     /translation="MKKRRIVLILSSLLVTNIFPIIGYSLELNQLEHGNRRDEESKLT
                     STFDSLIESNQTQESSEDLSISSSGETTDNVEAEGSDKQGLEDAPISAVELPDDPNII
                     SIDKIFQTPIGTSTSILENGKLLQLNPAERSQRGAIWSNKKISLLSDFTFKSYLYLGN
                     QRDRAGDGMTFTLTGDPRMASNPSQVIGSPGMGIGSYSTRSGQPYIANALSIEFDTYK
                     NSGSSDRMDREITADNRGHGHVAFVTPKPNNNNYSGEHTGVTVAPTFLSNGTWRLLII
                     KWDAEQRSLTYDLEGVGHSSRIIPDLIRQFGSTEVNWGFTSSTGTFFQENAVAVTRLP
                     SNVTSTADVKVNDSDYGAIAEAGGNDKITIRNTLKVEGDFLEYKKMQTSIDLPQELTI
                     PEHILINGNQIDAKDILITGNRVTLNLTDYFIAGDTLIIELETTLQEVIPEKVLTMHF
                     EYLENGNLIEQSNEINLKIAKLKEIDIHVYYKDKDSQIDIAEMKTITGKIGEKYKETP
                     IDIEGYVFDSDSANTEGIFSENTEDIYFYYRKGKLYLSTAPQYVNFGKHKISSRPLSE
                     FGQFSGGLKIIDERAFGSWKLQLKQEKVLTNGDFELPEALSFVTSENTTVIGTSAVTI
                     FESDQKGESDLSSLLDSTAHRGIRIDIPVENQRIGTFEGKLSWILADVPGN"
     gene            6486..7583
                     /locus_tag="IV334_002401"
     CDS             6486..7583
                     /locus_tag="IV334_002401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 and DUF3324 domain-containing protein"
                     /protein_id="HAP8944430.1"
                     /translation="MIKGAIFLRPKQQLFITLLVILGALFNNISVSASAFNFSVTPIT
                     SENQIDKRKTYFDLQLDPDQEVEVKAELRNDTEKEVKIDISVNSATTNSNVMVEYGKN
                     EIEKDMSLIFDLVDYVSYPQIVTLKPKSVQTVAFHVRMPNERFDGVLAGGITFQEIQT
                     EKDQTETKDQSLSIENEYTYIIALLMQQTLNEVAPNLTLHEVKPDQINARNVILANVQ
                     NDQKTYINQVVIETKITKKGHSEVLYQEEKEGLQIAPNTNFSFPTALNGQPLTPGEYH
                     LTMTVLGNENENGKFSRKKGNTTINYTNQWVFEKDFTIDGKVAKELNTTDVTIKKDNN
                     WIYILVASLFLLFILLCILLFVLRKKEEKEK"
BASE COUNT         2436 a         1390 c         1644 g         2157 t
ORIGIN      
        1 gttgttaata atccgtgaac aaaaagtaat tctttttcca atagttgtta aaggtttttt
       61 attttttata gtattttttg ttatttaaga aaggaataag gttaaacaaa taacaggttg
      121 ctttttgggt ctttttccgt tctagaaact gaacgaagaa aagggaaagt tgtctctttt
      181 gtgataagag aaatcataat aaacgaatga cttactttcc aataaattga gaacagtcag
      241 aaccagacaa attagttgca tctgatgaat aaagtgatta ttctatgaca tgaggtgata
      301 cagatgaaat tagaagtgac aaaagaagca caagaagtat tgaagaataa attagaagca
      361 gacgatcaac ttttattaga tatcgaaaac ggtgacggtc catttgcaga aagccaagtc
      421 agttgccaat tggatacggc atttcgattg atcattgtca aaaaagatac acaaacagat
      481 ctaagtcttt attctgtagt ggctgataca ccagttggac caatcaaaat caaagacagt
      541 gcaatacttt acatggatga tccatccaca cttgctttag aaccaactta taatagttta
      601 caattaaaag gaccaagcgg cttgagaaaa ggcaacttgc aagttgttcg aaaagactag
      661 agggataaaa tgggaaaatt ggcgatcatc agcgacctac atgtggacat caataaattc
      721 ggcgaatcag agctgatgca gctttggcaa gttttgcaaa agcacaagat cactcggctt
      781 catctagccg gagatacggc taataaagta gaccgttgcc tagctgttgt cgaatttttc
      841 gtcaataaag gattatctac gacatttcat ttcggcaatc acgagttggc agatgtgccc
      901 aatgaacaga tgatggacca ttttccagac ccacattttt taaatgaacg gtatattgct
      961 ttaaatgagc agaccgttct tttagggatg aatggctggt acgactatca gttttccgaa
     1021 ttgaaagatt acgaggcaat cgtacgaatg aagcgtttgt attggtatga tcggttcatc
     1081 cagcgtggga aaacagatct cgaggtcaat gcagaaatgc ttgcagctgc aaaaaatttg
     1141 ctagaccagc tggaagcaaa aaaacttgag gtgattttat ccactcattt cgttcctaaa
     1201 cgggcattta tcatgtatca atctgctcct tatgagcgtt ggaacaaatt aaatgcattt
     1261 ttaggatcag aatcattcgg agaattactt gaccgttatt caaatatcaa acaagtagtt
     1321 tttggccata cgcatcgtcg ctttgaagat aaatccatcg gtcacacgat ttacagctgc
     1381 agaccttttg gttattatta tgaatggcag ctgacccgag attttgtctt gaaagaacag
     1441 ttgctagaca gctatgatcc tatgaaatta cgtgtcctct tgcgtgccca tcacccagct
     1501 tttcaggcct atcaaacaca acatttgcaa gaggaatttg aacaagcgat gacaatcgtt
     1561 ccgtattgag acagcattag taatgtgtaa aagaaaatac cttgtaaaac ataaggataa
     1621 gtaagagcag tagatccatt tatcgtggat atgctgcttt ttttgcggaa aataatcagc
     1681 gaatgtttat ttgatcggat gatttgtaac tcgcagagta ctagatgcaa gcgtgtattc
     1741 tttttttgaa cgataatcac aaaaaaaaat ataatcgtaa atttcaagtt taagttagcc
     1801 acccatggtg acccggttgc tcgttaccac caaccgtcta atttttctaa tcgagtgggc
     1861 ccttgacaat gagctgaggc atggtagtct cccgactggc tggccccggc cagctttcca
     1921 gacacaagcc atgccgaggg aggctcgttg tcaagggccc acgtgtcaag ccgtcaaccg
     1981 ttcctgccta aatgcctgga cgaacgtgtc atgcggcgtt gcatagccca aaatctttcg
     2041 cgggtaatcg ttcatccaac gctgaatccg caagatttgg ttttcgctca cgttgccgat
     2101 tgggctgccc ttggggatga aacggcgaat cagcttgtgt tggttttcgc tcgtcccgcg
     2161 ctcccaggaa gcataggggt ggctgaagta gatgtttgcc acatcctgca aggcatcgtg
     2221 cagtcccgcg aactccgagc cgttgtccga ggtaaccgtc ttgaacagct ggccgaagta
     2281 cgcaccagcc cgttctctca gctgtcggac cgcttggttg accgattcgg catccttccc
     2341 ggccagcttc agaatgacct cgaagcgtgt ctgccgttcc accaacgtca gcagcacggc
     2401 atccgacttg gtcttgtttc ctacgaccgt gtcgacttcc caatggccga aggttttccg
     2461 ggtaccaacc gcctccgggc gctcttcaat ggatttccct agcacccgtt tattcgggcg
     2521 gttgccatga cgggacgatt tcggtttgcg ggacagtttt tctagcaggt ccatattctt
     2581 cgttttcatg atgccacggt cgatccactg atagagggtg gtggtcccag ggataatggc
     2641 cgggtcgaac aattcgtgtt ttctggcaaa gcccaccacc acgtccgggg accatttttc
     2701 caacagcatc atgtcgtccg cccattcaat gaaggcgtcc gtgtcggccc ataagggccg
     2761 tctgccgcag ttcaagcggt tcttctcgta ggccgcctgc ccggcatcgg cgtcgtagac
     2821 gtcatgataa tagtcgtaga ccttcccgtt ctgattctgg cggttaagct gagtaacggt
     2881 cccgcgtttg atttcgttgt ttatggtctg cggcacgcag cccaggacat ccgcaacctg
     2941 acggttggaa tagttttcct ttttcaggat ggcgatttgg caacgttctg agtaggttag
     3001 gtgttttccc ttgcgtgaaa ctgtgttatc atggttttgc gtcatgtgaa ttcatccttg
     3061 tctattgaga gttgtggtaa cttcaatata acatggaatt cacgtggcgt ttttttattt
     3121 ccaaatggct aagttaatta taaaatccgc caaaaaaata taatatacat tttttattta
     3181 attataatta atattgattg aagtctaggc agtcaagtca cattttgata gataggaggg
     3241 ggacttacga attattttaa aggctatgac agctgtattt ctagattgtg tttgcatcaa
     3301 gttgcaataa aaactagaaa tcaaatcggg ctgataatca tttataacta atcgatacat
     3361 aggaccaaat ataggtctat gttcttttat ttcataagta tcgtaaaaca gaattttgct
     3421 atggattaat ccaatcgact gatatgttta gtaaataaag aaagaggaat gtcaatgatg
     3481 aaaaaagaaa tcgtattaac agcagcaatg atgttattca gcatggtagc aagtacgact
     3541 tttgtttctg caactgaagt atacccaaag gaatataaca cagaaggaac gattacattt
     3601 gaagcaggag atgagggtgt aactcctcct gttgatccag agaatcctga tcccaataaa
     3661 cctgtcgatc catcagatcc tccaagccct ggaacaggag gggcattatc gattgattat
     3721 ggatcaaaat tcaaatttgg gactcaaaaa atttctactg cggataaaac gtattatgca
     3781 gctgctgatg tgatgaacga tgggtctaga aaaccgacat atgtccaagt gacagatagg
     3841 agaagtacct tatctgggtg gaaattaagt gtctctcaac cagaacagtt taaaactgcc
     3901 agtggggatg aactagttgg agcacagctg aaatttacga aagggcaggc tgtttctttg
     3961 gttgatccaa catatacgcc gcaaacggtg aattctgaat taacgttgac accgggagga
     4021 aacaatacgt tagcaattaa tgctaaatca ggaacaggtg tggggacttg ggtctatcga
     4081 tttggtgcaa atgaaaatga aaatcaagat gcggtacagc tttcggtacc cggtaaatct
     4141 gtcaagttag cgcaacaata ttccactaaa ttagtttgga cattagaaga tacacctaac
     4201 aactagttta ctgaaaagaa ctgttaaaaa taaatacgta gtcatattgt cttgcgaggt
     4261 aatcgagcaa aaattgtcaa aggctaggct aaatttgttt ggatacgaat agttcatcta
     4321 ggaatttaac gatcaggagg aaaagatata ttgaaaaaga ggaggatcgt gctcatttta
     4381 agcagtctct tagtgactaa tatctttccg atcatagggt actctttgga attgaatcag
     4441 ttggagcatg gaaatagaag agacgaagaa agcaaactca cttcgacatt cgacagttta
     4501 atagaatcca accagacaca agaaagcagt gaggatttaa gtatttcgag tagtggagag
     4561 accactgaca acgtagaagc tgagggttcg gacaaacaag gactagaaga tgcaccaatt
     4621 tctgcggttg agctgcctga cgatcccaat attatttcta tcgataaaat ttttcaaaca
     4681 cctatcggta ctagcacgtc catattggaa aatgggaagt tactgcagtt gaacccagca
     4741 gaaaggtctc aaagaggagc tatttggtca aataagaaga ttagtttact ctcggatttt
     4801 acatttaaat cttatttata cttaggaaat caacgagata gagcgggaga tggcatgacc
     4861 tttacattga ctggcgatcc tagaatggct agtaatccca gtcaagttat tggatcgcca
     4921 ggtatgggaa ttggctcata ttctactcgt tctggccaac cttacattgc gaatgcgctc
     4981 tcgattgagt ttgatacgta taaaaatagt ggttcttctg atcgaatgga tcgagagata
     5041 actgctgata atagagggca tggacatgtc gcttttgtca cgcctaaacc aaataataat
     5101 aattattctg gagagcatac tggcgtgacg gtagctccta catttctttc taatggcaca
     5161 tggcgcttgt taataattaa atgggacgcg gagcaaagaa gtcttactta tgatttggaa
     5221 ggtgtcggtc actcttctcg gattatacct gatctcatta ggcagtttgg ttctacagaa
     5281 gtaaattggg ggtttacttc ttcaacaggt actttttttc aagaaaatgc tgtcgctgtc
     5341 acgcgacttc catcgaatgt cacatcaaca gctgatgtaa aagtcaacga tagtgattat
     5401 ggggccatcg ctgaagctgg aggaaacgat aagataacta tcagaaatac attgaaggtc
     5461 gagggggatt ttcttgaata taagaaaatg cagactagta tcgacctccc acaagaatta
     5521 actattcccg agcatatctt gatcaatgga aatcaaattg atgccaaaga tatcttgata
     5581 accggaaatc gagtcacgct aaatctgaca gattatttta ttgctggaga tacgttaata
     5641 atcgaattgg agacaacttt gcaggaagta attcctgaaa aagtactcac tatgcatttt
     5701 gaataccttg aaaacgggaa tttgattgag caatcgaatg aaataaacct aaagatcgca
     5761 aagctgaagg agatagatat ccatgtttat tataaagata aagacagtca gattgatatt
     5821 gctgaaatga aaacaatcac tgggaaaata ggagagaagt ataaagaaac tcctattgat
     5881 attgaagggt atgtttttga ctcggattca gcgaatacag aaggcatctt ctccgaaaat
     5941 acagaggata tctattttta ttacagaaaa ggcaaacttt atctctcaac tgctcctcaa
     6001 tatgtcaatt ttggaaaaca taaaatatcc agtagaccat taagtgaatt cggacagttt
     6061 tcaggtgggt tgaaaattat agatgaacgg gcttttggaa gttggaaatt acaattgaaa
     6121 caagagaaag ttcttacaaa tggagatttt gagcttccag aagctctttc ctttgttacc
     6181 tctgaaaata caacggttat agggacatcc gctgttacga ttttcgaaag tgatcaaaag
     6241 ggtgaaagcg atttgtcctc tcttttagat tctacagctc atcgtggaat cagaatagat
     6301 attcctgtag aaaaccaaag aatcggaacc tttgagggga aactatcttg gatacttgca
     6361 gatgtcccag gaaattaaca ggaagagaag aatataacaa aattattgag tttcaagaag
     6421 ggcacaggta ccataggctc ttgtgtccca cagagacaag attttgttat agtcttctct
     6481 tacatatgat taaaggagcg attttcttga gaccaaaaca acaattattt ataacactcc
     6541 tagttatttt aggggcactt tttaacaata tatcagtgtc agctagtgca tttaattttt
     6601 cagtgacgcc gataacttca gagaatcaaa tcgacaagag aaaaacttac ttcgatcttc
     6661 aacttgatcc ggatcaggaa gtagaagtaa aagcggaatt gagaaatgat acagaaaaag
     6721 aagtgaaaat cgatatttct gtgaatagcg caaccaccaa ttcaaatgtg atggttgaat
     6781 acggaaaaaa cgagattgaa aaagacatga gcctgatctt tgatctggta gattatgtga
     6841 gctatccaca gatagtgacg ctaaaaccta aaagtgtcca aaccgttgct tttcatgtca
     6901 ggatgccgaa tgagcgattt gatggtgtat tggctggtgg gatcacattc caagaaatcc
     6961 agacggaaaa ggaccaaaca gaaactaagg atcaaagctt atcgattgag aatgaataca
     7021 cctatattat tgctttgttg atgcaacaga cactgaacga agttgctccg aaccttactc
     7081 ttcatgaagt taaaccagat caaatcaatg caagaaatgt catcttagca aacgttcaaa
     7141 atgatcaaaa aacctatatc aatcaagtag ttattgaaac aaaaattaca aaaaaaggac
     7201 attctgaagt tttatatcaa gaggaaaaag aaggattgca gattgcgccg aatacaaatt
     7261 tttcttttcc aactgcttta aatggtcaac cgctaacccc aggtgaatat caccttacaa
     7321 tgactgtttt aggaaatgaa aatgaaaatg gaaaattttc gagaaaaaaa ggaaatacta
     7381 cgatcaacta taccaatcaa tgggtatttg aaaaagattt tacaatagac ggaaaagtag
     7441 ccaaggaatt gaacacgacg gatgttacga ttaaaaaaga caataattgg atttatatct
     7501 tagtagcttc acttttctta ttgtttattt tattatgcat acttttgttt gttttgagaa
     7561 aaaaagaaga gaaagaaaag taaggttaag ataacaaata gtaaattttt attattgtag
     7621 gatggtt
//
LOCUS       DACDKM010000115         7607 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20834_2016
            SAMN12640370-rid9891663.denovo.115, whole genome shotgun sequence.
ACCESSION   DACDKM010000115 DACDKM010000000
VERSION     DACDKM010000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640370
            Sequence Read Archive: SRR10040891
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7607)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7607)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742835.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742835.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:04:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,012
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 29 of 75
            Pseudo Genes (incomplete)         :: 33 of 75
            Pseudo Genes (internal stop)      :: 19 of 75
            Pseudo Genes (multiple problems)  :: 5 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7607
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640370-rid9891663.denovo.115"
                     /isolate="E20834_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(239..916)
                     /gene="nth"
                     /locus_tag="IV334_002402"
     CDS             complement(239..916)
                     /gene="nth"
                     /locus_tag="IV334_002402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HAP8944431.1"
                     /translation="MLSKQKTMEALETMYGMFPEAHGELKHNNPFELLIAVILSAQAT
                     DVSVNKATPDLFASFPTPDALAEASIDEIILKIKTIGLYRNKAKNIKACAQQLIERFD
                     GQVPTSREELMSLPGVGRKTANVVLGDAFGIPAIAVDTHVERVSKRLRICKLDATVME
                     VEETLMRKVPQELWVKTHHTLIFFGRYHCTARNPKCEVCPLLSICQDGKNRMRLKEKA
                     LKKKPRL"
     gene            complement(923..1621)
                     /locus_tag="IV334_002403"
     CDS             complement(923..1621)
                     /locus_tag="IV334_002403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain protein"
                     /protein_id="HAP8944432.1"
                     /translation="MLNLENYLSAGQTVISNLLLKNYHRIGLQSEEFLFVLQLHMAQL
                     EGDTFPDLQMIAMDMGIKQDQIFQILDRLVTNGFIKIETTIVNGKKADRYNLYPIYDL
                     LGEYLKTQEKKYEEKTQEKEIQSVYQLFEQEFGRPLSSIEFQRIGQWLEEDHYQPEIL
                     KLALREAVLNQAYSFNYVDRILLSWERKNLRTKQQVEEDQKRRKQQLLQREVEQSAQQ
                     EELPKVSLKNWLEE"
     gene            1889..3073
                     /locus_tag="IV334_002404"
     CDS             1889..3073
                     /locus_tag="IV334_002404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance MFS efflux pump"
                     /protein_id="HAP8944433.1"
                     /translation="MYSFTNKHSKVFGYVSVLFTGLGFTIVSPVLPFLTLPYSHSIHQ
                     QAFYITLLMSVYALAAFLSAPILGSLSDHFGRHPILIISLLGSSIGYLLFGLGNSIWM
                     LFLGRIIEGLTAGEISTLYAYFADITEPNERTKVFGWMGALVGIGTTLGPIIGGLLAE
                     LGNSVPIFIGALFTFLNAVYGYTFMLESLPIKKRSVDLSFSHVRPFHQLQQLFKISSV
                     IPLLTAGFAVWLAAGSLQSIFSQFSIDAFQWKAGLVGLSFSLIGILDTVSQLFIMPRL
                     LKKFSEQQITRIGMFSEILAYLFITLSGILLLPILFLFGIICYGFGDSIFTPVFNGQL
                     SNSVSDNQQGLVMGGTQSIQSLSRVIGPLIAGQLYAAAPFLPTVFGFLLMIFAYYHYN
                     KK"
     gene            complement(3079..3522)
                     /locus_tag="IV334_002405"
     CDS             complement(3079..3522)
                     /locus_tag="IV334_002405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAP8944434.1"
                     /translation="MDKRQLLIEEYLDLANEIIHRNKPEMEKEFAGFTLNELEVIEHI
                     GKIPDPNVTKLANASYMTRGAISKLTKKLIARGIINTYQSEENKKEIYFQLTPIGQKI
                     RNRHEQLHQKWVQRDATVFENMSKEEFDTVFRFIGRYREFIRSSK"
     gene            complement(3748..4035)
                     /locus_tag="IV334_002406"
     CDS             complement(3748..4035)
                     /locus_tag="IV334_002406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8944435.1"
                     /translation="MNKEYFVQQDAFLEDYKSASKKLTINIDELADQVLSQLNETQEE
                     YYTMPANDAKDNVSHRFPFSRRIYSDENDGTLNTEYTYEGQPYVLETDDDL"
     gene            complement(4206..5558)
                     /locus_tag="IV334_002407"
     CDS             complement(4206..5558)
                     /locus_tag="IV334_002407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH dehydrogenase"
                     /protein_id="HAP8944436.1"
                     /translation="MKVVVIGCTHAGTAAVKSILKNHPEAEVTVYERNDNISFLSCGI
                     ALYVGGVVKDPAGLFYSNPEELSSLGATVKMEHDVTDVDTENKTVTAKDLKTGETETV
                     SYDKLVMTTGSWPIIPPISGIDAKNVLLCKNYNQANEIIAQAKEAKRVVIVGGGYIGI
                     ELVEAFVESGKQVTLIDGLDRILNKYLDKPFTDILEKELTDRGVTLALGENVTEFITD
                     EEGKVKQVATPTDTFDTDMVILCVGFRPNTKLIEGKVDTLPNGAIKVNEYMQTSNPDI
                     FAAGDSAVVNYNPSGTQNYIPLATNAVRQGLLVGNNLTDHKMAYRGTQGTSGLYLFGW
                     TIGSTGVTKESATLNDLEVQATVFEDNYRPEFMPTTEKVMMELVYEKGTNRIVGAQFM
                     SKYDITQSANTMSLAVQNKMTVEDLALSDFFFQPHFDRPWNYLNLLAQAALGEIETAQ
                     "
     gene            complement(5831..6277)
                     /locus_tag="IV334_002408"
     CDS             complement(5831..6277)
                     /locus_tag="IV334_002408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HAP8944437.1"
                     /translation="MENKLVEEVIEKLKKENIRITPQRYAVLEYLIEHHSHPTADEIY
                     KALEHRFPNMSVATVYNNLRLFTSIGFVQEMKYGDASSRFDFSSKRHYHVICENCGKI
                     VDFHYPGLEDIEMAASRLTDFEINEHRLELYGLCPDCKESAKKAQM"
     gene            complement(6513..7439)
                     /gene="cysK"
                     /locus_tag="IV334_002409"
     CDS             complement(6513..7439)
                     /gene="cysK"
                     /locus_tag="IV334_002409"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HAP8944438.1"
                     /translation="MTAIYNSVTELIGKTPIVKLNKIVPEDSADVFVKLEFFNPGGSV
                     KDRIALSMIEKAEHDGLLKPGDTIIEPTSGNTGIGLSMVGVAKGYKVIIVMPETMSIE
                     RRLLMKGYGAELILTPGADGISGSIREAERLAKENGYFLPLQFENEANPLVHEKTTGP
                     EIHQAFGVNGLDAFVAGIGTGGTITGAGRELKRVYPKIELIGVEPAESAILEGKEAGP
                     HKIQGIGTGFVPKTLDTSVYDKVLSISGDEAMETAREVGRKEGILVGISSGAAIAAAL
                     KVAKELGKGKKVLAVVPDNGERYLSTALYQEI"
BASE COUNT         2053 a         1556 c         1252 g         2746 t
ORIGIN      
        1 atcctttgga atactggact tattttatag gtatattgga aggcattatt ttctacatcg
       61 ggtattttcc caataaaaat aacaactttt atttttatcg caataaattg taataagtta
      121 ccatcctagc tactgagtat gtacatttac tttttcatgc aaaaaagata aacgaaacag
      181 cttttctcca taataacaag ataattcata catttatttg caattaaaga aaccacgtct
      241 acaggcgtgg tttctttttt agcgcctttt cttttagacg catccgattt ttcccatctt
      301 gacatattga tagcaaagga catacctcgc acttgggatt tcttgccgtg caatgatatc
      361 taccaaaaaa aatcagtgta tgatgtgtct ttacccataa ttcttgtggt actttccgca
      421 tcagtgtttc ttctacttcc atgaccgtag catctagttt gcagatcctc aggcgttttg
      481 agacacgttc cacgtgggta tctactgcga ttgctggtat cccgaatgca tcccccaata
      541 cgacatttgc tgtctttctg cctacaccag gaagagacat caattcttct cttgacgttg
      601 gcacttgccc atcgaaacgt tcaatcagct gctgtgcaca cgctttgatg ttttttgctt
      661 tatttcgata caaaccaatc gtttttattt taagaatgat ttcatcgatt gaagcttctg
      721 ctaacgcatc gggtgtcgga aaagaggcaa acaaatctgg cgttgcttta ttcactgata
      781 catctgtcgc ctgggcactt aagatcactg caatcaataa ctcaaatgga ttattgtgct
      841 tgagttctcc atgtgcttcg ggaaacattc catacatcgt ttctagagct tccattgttt
      901 tttgtttgct taacatattc cgctactcct ccaaccaatt tttcagtgag actttcggca
      961 attcttcttg ttgagctgac tgttctactt ctctttgaag aagctgctgt tttcttcgtt
     1021 tttggtcttc ttccacttgt tgttttgtac gtagattttt tctttcccac gatagcaaaa
     1081 tgcggtcaac gtaattaaaa ctataagcct gatttaatac ggcttcccgc agtgctagct
     1141 tcaaaatttc cggctgataa tggtcttctt ctagccattg tccgatccgc tggaactcga
     1201 tcgaagaaag cgggcgtcca aattcttgtt caaataattg atacactgac tggatttctt
     1261 tttcttgtgt tttttcttcg tatttttttt cttgtgtttt taaatactca cctaatagat
     1321 catagatggg gtaaagatta tatcgatctg cttttttacc gttgacaatc gtcgtttcta
     1381 tcttgataaa tccatttgtt acgagacgat ccagtatttg aaaaatctga tcttgtttga
     1441 ttcccatatc catagcaatc atctgcaaat cagggaaagt atctccttca agctgtgcca
     1501 tatgcaactg tagaacaaat aaaaattctt cagattgtag accgatccta tgataatttt
     1561 taagtaaaag attagatatg actgtttgtc cagcgcttaa gtaattttct aaattcaaca
     1621 tgatttctcc tccgctcaat cacagtatac gtaaaaaaaa catgggaagc aagaacaggt
     1681 ttcttttcta tttacttcac tgatcggtca gatttacaat gtcgtctact tgatttttgt
     1741 ttgttcatgt ttttgatgcg tatcttcact tttcggaata aataaattaa gtagatttcg
     1801 ctgttcctta gaatgttgac aaggaaacaa aattactata cactattttt gtttccaagt
     1861 taacattatt gcataaagga gtttttacat gtacagtttc acaaataaac attctaaagt
     1921 ttttggatac gtctctgtct tattcactgg attaggattt actatcgtca gtcctgtact
     1981 tccgttttta acgctgcctt attctcactc catacatcag caagctttct atatcacttt
     2041 gctcatgtct gtttatgctc tagctgcctt cttatctgcc cccattctcg gtagcttaag
     2101 tgatcatttt gggcgtcatc cgatccttat tatcagttta ttaggatcaa gtatcggata
     2161 tttgcttttt ggtttaggaa attcgatatg gatgttgttc cttggtcgta tcattgaagg
     2221 attgacggca ggagaaatct ccaccttata tgcttacttt gccgacatca cggaacctaa
     2281 tgaaagaacc aaagtttttg gatggatggg tgctttagtc ggtataggaa caacgctggg
     2341 tcctattata gggggtctat tagctgagtt ggggaattct gttcctatat ttattggagc
     2401 actctttact ttcttaaatg ccgtttatgg ctacacattt atgctagaaa gccttccaat
     2461 aaaaaaacgt tcagtagatt taagtttctc ccatgtaaga ccttttcacc aattacaaca
     2521 attattcaag atttcatccg ttattccttt attgacagca ggttttgctg tttggttggc
     2581 agctggctct cttcaatcta tcttttctca attttcgatt gacgcctttc agtggaaggc
     2641 tgggttggtt ggtttaagct tctctttgat tggaatcttg gatactgtct cgcaattgtt
     2701 cattatgcct cgattgctca aaaaattttc agaacagcag atcacaagaa tagggatgtt
     2761 cagtgaaatc ctcgcctatc tattcatcac attatctgga attcttctac tgccgatttt
     2821 gtttttattt gggattatct gttatggttt cggtgattct atttttactc ctgtattcaa
     2881 tggccaacta tccaattctg tctcagataa ccagcaaggt cttgtaatgg gcggtaccca
     2941 aagtatacaa tctctatcgc gcgtcatcgg cccgctaatc gcaggacagc tttatgctgc
     3001 agcaccattt cttccgacgg tattcggatt tttgcttatg atttttgcct attatcacta
     3061 caataaaaaa tagtagcttc atttgctact tcgtataaat tctctgtatc gaccgataaa
     3121 acgaaatact gtgtcaaatt cttcttttga catattttca aatacagtag catctcgctg
     3181 tacccatttt tgatggagtt gctcgtgtct attccttatt ttttgtccga ttggcgtcaa
     3241 ttgaaaatag atttcttttt tattctcttc gctttgataa gtgtttatga tccctctagc
     3301 tatcagtttt tttgttagct tactgatggc tcctctagtc atgtaactag cattcgctaa
     3361 ttttgtcacg ttaggatcag ggattttccc gatatgttca atcacttcta gttcatttag
     3421 agtaaatcct gcaaattctt tttccatttc tggtttatta cgatgaataa tctcattagc
     3481 taaatccaga tattcttcta ttagcagttg tcttttatcc atttctacac cttctttttc
     3541 tttcattata ttgttttttt gtttcctatt caacaaagat aaaaatattt tgtaggaaaa
     3601 cccaatctga aaccaagaaa aaaaggggtt gacaaagctt gtcataaccc ctttttctga
     3661 ttaaaagata gtaaagagac agtcatcggt aattgtgatg attttttgtc atcacttaag
     3721 gatgagcggc cttttcaaaa actgtcttta aagatcatca tcagtttcta gcacatatgg
     3781 ctgaccttca tatgtgtact ctgtattcaa tgtgccgtca ttttcatctg agtagatgcg
     3841 tctactgaaa ggaaaacgat gagaaacatt atctttggcg tcatttgctg gcatcgtgta
     3901 atattcctct tgcgtttcat tcaactgaga gagtacttga tctgccaact catcaatatt
     3961 gatcgttaat ttcttcgaag cacttttata atcttctaaa aatgcatctt gttggacaaa
     4021 atattcttta ttcatcttct ttctcacctt tcttttcaaa atcaaataaa gctgcttgtt
     4081 cttttatgta acagctttat ctttgctgct tattgtgaca aaaaattcga agaaagacaa
     4141 ctaattgtac ttttcacaaa aaaagacaga gggtcgtgac aaaattgtgt cacaaccctt
     4201 tttctttatt gtgcagtttc gatttcgcct agcgctgctt gtgcaagtag gttcaagtaa
     4261 ttccatgggc ggtcaaaatg tggttggaag aagaaatctg aaagtgcgag atcttctaca
     4321 gtcattttat tttgaacagc taaagacatg gtatttgctg attgtgtaat gtcgtatttg
     4381 gacatgaact gagcacctac gatacgattc gttccttttt catagactag ctccatcatt
     4441 actttttcag tagttggcat gaactctgga cgataattat cttcaaatac agtcgcttgg
     4501 acttctagat cgttcaaagt tgcgctttct ttggtcacac ctgtcgaacc gatcgtccag
     4561 ccaaataggt aaagtcctga agttccttga gtaccacgat acgccatttt atgatctgtc
     4621 aagttatttc ccacaagcaa accttgacga acggcatttg ttgccaaagg aatatagttt
     4681 tgcgtgccac ttggattgta gttgactact gcagagtctc cagctgcaaa aatgtctgga
     4741 ttacttgtct gcatgtattc attcactttg attgccccgt ttggcagtgt atctactttt
     4801 ccttcgatca gttttgtatt tggacggaag cccacacata aaatgaccat atctgtatca
     4861 aatgtatctg ttggtgtagc tacttgtttc acttttcctt cttcatctgt gataaattca
     4921 gtaacatttt cgcctagcgc aagagtaaca ccgcggtctg tcaattcttt ttctaagata
     4981 tctgtgaatg gtttatctag atatttattc aagatgcgat ctaatccatc aatcaatgtc
     5041 acttgttttc cagattcgac aaaagcttct actaattcaa taccgatata tccaccacca
     5101 acaattacta cacgttttgc ttctttcgct tgtgcaataa tttcatttgc ttgattgtag
     5161 tttttgcata ggagtacatt tttcgcatcg attccactaa tcggtgggat aataggccat
     5221 gaaccagttg tcatcactaa tttatcataa gaaaccgttt ctgtttctcc tgttttcaga
     5281 tcttttgccg ttactgtttt attttctgta tcgacgtctg ttacatcgtg ttccatttta
     5341 actgttgctc ctaatgaaga taattcttca ggatttgagt agaacaagcc tgctgggtcc
     5401 tttactactc cgccgacata aagtgcgata ccgcaagata agaaagaaat attgtcattt
     5461 ctttcatata ctgtcacttc tgcttctgga tgatttttta aaatactttt tacagctgca
     5521 gtacctgcat gagtacagcc aattactact actttcattt atggtccctc cgattaacat
     5581 tttgtccttc gacaaaacta ctataacaga aataaaacgg atacaacagt ttaatgcttg
     5641 tgaattcaca aaggtttgtt tgtagtttca ttccttttca agcataagaa cccaatatct
     5701 acaagattct tattttaata atattcactt ttatatctaa gattgtgaat aaaagacaaa
     5761 aaaataaaaa agctagcgtt ttttttacac agctagcttt ttttaggtat aacacttgta
     5821 gaaactgttt ttacatttgc gccttttttg ctgactcttt acaatccgga cagagtccat
     5881 acaattccaa ccgatgttca ttaatctcaa aatcagtcag tctgcttgca gccatttcta
     5941 tatcttcaag ccctgggtaa tgaaaatcaa caatcttacc gcagttttca caaattacat
     6001 gataatgacg ctttgaactg aaatcaaatc ggctagaagc atcgccgtac ttcatttctt
     6061 gaacaaatcc aatcgaagta aataagcgaa ggttattgta aacagtcgca acactcatat
     6121 ttggaaaccg atgttcaagt gctttataaa tctcatcagc cgttggatgg gaatgatgtt
     6181 caatcaaata ttctaaaacc gcgtaccgtt gaggtgtgat tcgaatgttc tcttttttta
     6241 acttttcgat aacctcttcg actaacttat tttccatgta aacaccccac gttgttattc
     6301 tcgtcttatc atactatttt tacattttaa aatccactta acatgaaaag aaaattcaat
     6361 tttattgtat tttaatgaaa aaagacgtaa aacagtcgcg ttcactattc tacgtctcta
     6421 ttcaaataat ttttctcatc ataaattttt gtgtgttctt ttcatcttta aaagtttttt
     6481 acataaacct atctggagaa aaaccaagtg ccttatatct cttggtaaag ggctgtagat
     6541 aaataacgtt caccattgtc gggaaccaca gctaatacct tcttgccttt tcctaattct
     6601 tttgcgactt ttagcgcggc agcaattgca gcgccagatg agatgcctac taaaatccct
     6661 tcttttcgcc caacttctct agctgtttcc atcgcttcgt ctccactaat agaaaggact
     6721 ttatcataca cagaagtatc cagtgtttta ggaacaaaac ctgtaccgat tccttggatt
     6781 ttgtgaggtc ccgcttcttt cccttctaaa atagcagatt ctgctggttc taccccgatt
     6841 aattcgattt tcggatacac acgttttaat tctcggccag cgccagtgat cgttcctcct
     6901 gtaccgatac ctgcaacaaa cgcgtccaag ccatttactc caaatgcttg atgtatctca
     6961 ggaccagtcg tcttctcatg cacaagagga tttgcttcat tttcaaattg taatggtaaa
     7021 aagtagccgt tttcttttgc caaacgttct gcttctctga ttgatccaga aataccatcc
     7081 gcaccaggag tcaaaatcaa ctctgcgccg taccctttca tcaataaacg tctttcgata
     7141 ctcatcgtct caggcatcac gatgattact ttatatcctt ttgcaactcc taccatcgac
     7201 aatccgattc ctgtatttcc agaagtaggt tcaatgatag tatcacctgg tttcagtaga
     7261 ccatcgtgtt ctgctttttc tatcatactt aaagcaatac gatcttttac acttcctcct
     7321 ggattaaaaa actcaagctt tacaaaaaca tctgctgaat cttctggtac gattttattt
     7381 aatttgacga ttggcgtttt gccaatcaat tcggtgactg agttatagat agctgtcatc
     7441 ttcaattcct ccttgtcttt gctcttctta cttatcataa tacaatctgt tcatttatgg
     7501 cgagtgattc gtctttgact agcaaaaaaa agacaaagat ataccatata tggtaatttc
     7561 ggctctataa tttatgggac tgatgaatca gaattgattc ttcagcc
//
LOCUS       DACDKM010000116         7559 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20834_2016
            SAMN12640370-rid9891663.denovo.116, whole genome shotgun sequence.
ACCESSION   DACDKM010000116 DACDKM010000000
VERSION     DACDKM010000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640370
            Sequence Read Archive: SRR10040891
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7559)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7559)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742835.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742835.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:04:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,012
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 29 of 75
            Pseudo Genes (incomplete)         :: 33 of 75
            Pseudo Genes (internal stop)      :: 19 of 75
            Pseudo Genes (multiple problems)  :: 5 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7559
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640370-rid9891663.denovo.116"
                     /isolate="E20834_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(50..1528)
                     /locus_tag="IV334_002410"
     CDS             complement(50..1528)
                     /locus_tag="IV334_002410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M protein trans-acting positive regulator"
                     /protein_id="HAP8944439.1"
                     /translation="MKLDYLIEEEDREKLLLARYLEFEKKNYVVLPKLYETLGLSKFK
                     TRNYIIELNKEFTEFKTKPEIQISEEAEIHVERLNLTDIKNLRRIYFQRSKIARLLTE
                     IVDSKVTIEKFAENHFLSISRAYVKRRELVSFLEQHQIKLKKNILVGEEINIRNVLFG
                     VYFGVFNGFSMPFRESVMNEAKSIVQYFEYLFNLKLSATKRIKFNLLTTISLLRAYGN
                     QPIGKRFFSDEYFKSSEGMKIIKDLSHISFLHIPKEQIIDEISYIMLFLITEEEFELD
                     GAFGLAEAHFHDIHMISQKMSVELIDQLPFSIEISEEERLYIRKELRMGMMHVNRKNW
                     IFDFEMTSFTSKKQLQFFFETYPIFSTAIQKVIQENKRYFLGKDERFMIRLFYDYLFL
                     LVGICPVSYFEAPIHVCIDFSQGSAYTDYIISQVKGFKNYNIVIESRITSRTNIYLSD
                     CLLEKFNKEQIIWKNPPTPDDWEYFGNTIIREKNRHLTEKIS"
     gene            complement(1826..2761)
                     /gene="murB"
                     /locus_tag="IV334_002411"
     CDS             complement(1826..2761)
                     /gene="murB"
                     /locus_tag="IV334_002411"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HAP8944440.1"
                     /translation="MNKEEIVESLATITILFDEPLMNYTFTKTGGPADVLAFPKKQEE
                     VKQIIDYCRIHDIPWMVLGNASNLIVQDGGIRGVVIMLTEMKQIHVKGTMVIAEAGAS
                     LIDTTYAALAESLTGFEFACGIPGSVGGAVYMNAGAYGGEIKDVFAEVDLLLEDGTLK
                     TLTKDEMVFSYRHSKVQELRAIVLEARFSLQTGDYEAIKARMDELTELRQSKQPLEYP
                     SCGSVFKRPVGHYTGQLIQQAGLQGLKWGGAQVSEKHAGFIVNIDHATATDYIELIAH
                     IQEVIKEKFDVSLETEVRIIGEKQAISETSRSLAN"
     gene            complement(2834..3754)
                     /locus_tag="IV334_002412"
     CDS             complement(2834..3754)
                     /locus_tag="IV334_002412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HAP8944441.1"
                     /translation="MKIGVIGVGNIAEKAYLPTYAKKQGTLDFYFATRNKETKERIQK
                     EYGFQHLYETIDELLEEKIEACMIHAATKVHYELAKKCLENGIHVYIDKPLSVHLNEV
                     RELQELADKNQIILMIGFNRRFAPMVEELKRIPDKRLIHLQKNRIAAKETTEFVLYDL
                     FLHLIDTAVYLLDEPILRTKYQIRETKEFLEYAILQLETQNQTAILTMDLASGANIEK
                     YQVTSKNGTYEVNDLTQLVIQEADGKKVKEFGDWTNTLVKRGFEPMVEAFFSQIRLGK
                     SETELLKQKEVVRSHELCEEVLKDHYRHQL"
     gene            3867..4619
                     /gene="xth"
                     /locus_tag="IV334_002413"
     CDS             3867..4619
                     /gene="xth"
                     /locus_tag="IV334_002413"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HAP8944442.1"
                     /translation="MKFISWNVNGLRAIVNKNFLEVFHELDADFFCLQETKLQAGQID
                     LDLPGYYQYWNYAERKGYSGTAIFAKKPALNATYGMGIDIHDTEGRLITLEYSDFFLV
                     TCYTPNSQSELKRLDYRLEWEEAFYNYLENLKKQKPVIVCGDLNVAHQKIDLKNWKTN
                     QKNAGFTPEERAALSRLLDNGFIDTFRYFYPTQEGVYSWWNYRFNSRKNNAGWRIDYF
                     LTSKDLEPGLADAKIHTDIMGSDHCPVELDLK"
     gene            5483..6016
                     /locus_tag="IV334_002414"
     CDS             5483..6016
                     /locus_tag="IV334_002414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease"
                     /protein_id="HAP8944443.1"
                     /translation="MNFVAMDFETANHQPYSACSLALVMVKNSQIVDEFYTLIQPETP
                     FFWRNVQIHGIHQEDVRNAPKFPEVWQTIQPYFQKNRLVVAHNAAFDTKVLAGCLDYY
                     HLEQPNYLSLCTVKSSRRLFPEFPNHRLNTVCENLNIQLDHHHDALEDSRACAEILLY
                     QEKKFGTDPLKKLVTIK"
     gene            complement(6506..7030)
                     /locus_tag="IV334_002415"
     CDS             complement(6506..7030)
                     /locus_tag="IV334_002415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP8944444.1"
                     /translation="MSEEVGIILREAVPGDAKDILLMMGQVNKETEFLVLDEAELLLP
                     PETLEEELDYIYESNNNLLLLAIYEGTIIGTASVKADSQFRLSHVGEVGISILQEYWG
                     MGLGTLMLEEIISWAKEMGVLFRLELDVQVRNERAVHLYRKMGFQIEAVMPRGARTDL
                     GEFLDVYKMSYLIE"
     gene            complement(7023..7496)
                     /gene="tsaE"
                     /locus_tag="IV334_002416"
     CDS             complement(7023..7496)
                     /gene="tsaE"
                     /locus_tag="IV334_002416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HAP8944445.1"
                     /translation="MIELSGLTMTEQFAEAIGHSAMPGDNLILTGDLGAGKTTLTKGI
                     AQGLGITQMIKSPTYTIIREYSQGRIPLYHMDIYRVAASGADLGLDEYFEGDGLSVVE
                     WGNLLEEALPEDYLELILEKSDTDLEKRYVKLISYGEQSETFKQRILEKWAENHE"
BASE COUNT         2229 a         1516 c         1238 g         2576 t
ORIGIN      
        1 acataaatca gctaataaat atgtgagaca aagctgccat ccataaatac tagctgattt
       61 tttcagttaa atgtctattt ttttctctga tgatggtatt tccaaaatat tcccagtcat
      121 ctggagtcgg tggatttttc caaattatct gttctttatt aaatttttca agtaaacaat
      181 cagataaata aatattcgta cgagaagtga ttcgactctc aatcacaata ttgtaatttt
      241 taaatccttt cacttgagaa ataatgtaat cggtataagc agatccttgc gaaaaatcaa
      301 tacagacatg aatcggtgct tcaaaatatg ataccggaca aataccgacc aataaaaaga
      361 gataatcata aaataagcga atcatgaaac gttcatcttt tcccaagaaa tagcgtttat
      421 tctcttgtat gaccttttgg attgctgtac tgaatattgg atacgtctcg aagaaaaact
      481 gtagctgctt tttagacgta aagctagtca tctcaaagtc gaatatccaa ttttttcgat
      541 tgacatgcat catacccata cgaagttctt ttcgtatata tagtctttct tcttcagaga
      601 tttcaatcga aaatggtaat tgatcaatca attccactga cattttttga ctaatcatgt
      661 ggatatcatg aaaatgagct tccgcaagtc caaatgcgcc atctaattca aattcttctt
      721 ccgtgatcaa aaacaacatg atataagaaa tctcatcaat gatctgctct ttagggatat
      781 gaagaaaaga aatatgtgat aaatccttga tgatcttcat tccttctgag ctcttaaagt
      841 attcatctga aaagaaacgt tttccgattg gttgattccc ataggccctc aacaaactaa
      901 tagtggttag caaattgaat ttgattcgtt ttgttgcaga cagttttaaa ttgaacagat
      961 attcaaaata ttgcacgata ctctttgctt cattcatgac gctttctcta aatggcatag
     1021 aaaaaccatt aaagaccccg aaatataccc caaataatac gttccgtatg tttatttctt
     1081 ccccaactaa tatgtttttc tttaacttta tttgatgctg ttcaagaaaa gagactaatt
     1141 ctcttctttt tacataggct cttgatatgc ttaaaaaatg attctctgca aatttttcga
     1201 tcgttacttt tgaatccacg atctccgtta acagtcgagc aatttttgat cgttgaaagt
     1261 agattcttct taaatttttg atatccgtca aattcagacg ttcgacatgg atctctgctt
     1321 cttctgaaat ttggatctct ggctttgttt tgaattcagt aaattcttta tttaattcta
     1381 tgatataatt tcttgtttta aatttagaca aacctaatgt ttcatataac ttaggcagca
     1441 ctacataatt ctttttctca aattctaaat atctagctag cagcagtttt tctctatctt
     1501 cctcttctat aaggtaatcc aacttcatat cctacacctc aaaaaatgcc tcataccaat
     1561 tacttgatat gcagatgaaa gttcatcttt tcacctatac tgattttatg atggattatg
     1621 cttgtttttt catcagttat tctcattttt tatctttttt ttgagaatat tgcaaaaaat
     1681 ggaaaaattt atgaggaaat attctatatt ttttgtttta ttgtgttaaa atgtacaaga
     1741 tttgcgccat ttgcgtaaac aaccacaaaa aagagcagga cggccaccgt cctgctcttt
     1801 tacttatttt aagttccatt acttattaat tggctaaact tctgcttgtt tctgatattg
     1861 cttgtttttc tccaatgatc cgcacttctg tttctaaaga cacatcaaat ttttctttga
     1921 ttacttcttg gatgtgcgca atcaattcaa tataatctgt cgctgtcgca tgatcgatat
     1981 tgacgatgaa tccagcatgt ttttcagaga cttgtgcacc gccccatttc aggccttgca
     2041 atcctgcttg ttggatcagc tgacctgtat agtgtcctac tgggcgctta aagacacttc
     2101 cgcatgaagg gtattctaat ggctgttttg attgacgcaa ttcggtcaac tcatccattc
     2161 ttgctttgat tgcttcataa tcccctgttt gcaaactgaa tcgagcttcc aatacgatag
     2221 cacgtaattc ttggacttta ctgtgacgat aagaaaatac catttcgtct tttgtcaaag
     2281 ttttcaatgt tccatcttca agcaaaagat ccacttcagc aaaaacatct ttgatctctc
     2341 cgccatacgc acctgcattc atataaaccg ctccaccgac acttcccggg atgccgcaag
     2401 caaattcaaa tccagtcaat gattctgcca aagctgcata agtcgtatca atcagtgagg
     2461 cacctgcttc cgcaatgacc atcgttcctt tgacatgaat ctgtttcatt tcagtcagca
     2521 tgatgactac accgcgaatc ccgccatctt gaacgatcag attgcttgca tttcctaata
     2581 ccatccaagg tatatcatgg attcggcaat aatcgatgat ttgctttact tcttcttgtt
     2641 ttttcgggaa tgcaaggaca tcagctggtc cacctgtttt cgtaaacgta tagttcatca
     2701 atggttcatc aaacaatata gtaatcgtgg ctaagctttc aactatttct tctttattca
     2761 ctgaggattt tcctttcttt tctcaaacgt cctcattatt ctatcacgct taatacaatc
     2821 ttgccagtct tgttcaaagt tgatgacggt agtgatcttt aagcacttcc tcacataatt
     2881 catgacttct tacaacttct ttttgtttaa gcaattctgt ttccgattta cctaaacgga
     2941 tctgagaaaa gaaggcctct accattggct caaacccgcg tttgactagc gtgtttgtcc
     3001 agtcgccaaa ttctttcact ttttttccat cagcctcttg aatgacaagc tgggtcagat
     3061 cgtttacttc ataagtacca tttttacttg ttacttgata tttttctata ttagcgccac
     3121 ttgctaaatc catcgttaaa atagccgttt gattttgtgt ttctaattgt aaaatggcat
     3181 attccaaaaa ttcctttgtt tctcttatct ggtattttgt tctcaaaatg ggttcatcta
     3241 ataaatacac agctgtatca ataagatgta aaaacaaatc ataaaggacg aactctgtcg
     3301 tttcttttgc cgctattcga tttttttgca ggtggatcaa ccgcttatct gggatacgtt
     3361 tcaattcttc taccatagga gcaaagcgcc ggttgaaacc gatcatcaaa attatttggt
     3421 tcttatcagc taattcttgc aattcacgaa cttcatttag atggacactc agtggtttgt
     3481 caatataaac atggatacca ttttctagac attttttggc taattcataa tgtacttttg
     3541 ttgctgcgtg gatcatacat gcttcgattt tttcttctaa aagctcatcg atcgtttcat
     3601 atagatgttg gaacccgtac tctttttgaa ttctttcttt tgtttcttta tttcttgttg
     3661 caaaataaaa atcaagtgtt ccttgttttt tcgcataagt tggtaaatat gctttttctg
     3721 cgatattccc tacaccgatt acaccaatct tcatctactt tcctcctgga tcatttagtt
     3781 actttcatct tacaccattt tttactaaaa tgaggtacaa tagtatcgca aaggtaagga
     3841 aattaattcg aaggagtgtg cttttattga aatttatctc ttggaacgta aacgggcttc
     3901 gtgccatcgt aaataaaaat tttttagaag tttttcatga gttagacgcc gattttttct
     3961 gtctgcagga aacgaaactc caagcaggac aaattgattt agatcttcca ggttattatc
     4021 aatattggaa ttacgcggaa cgaaaaggct attccggcac agcgattttt gctaaaaaac
     4081 cagcattgaa tgccacttac ggaatgggta tcgatatcca tgatacagaa ggccgtctga
     4141 ttactttgga atattccgat ttctttcttg taacctgtta cacgccaaat tctcaaagcg
     4201 aattaaaacg tttagattac cgattagaat gggaagaagc cttttataat tatttggaaa
     4261 atctaaaaaa acaaaaacca gtcatcgtgt gtggggatct aaatgtcgct catcaaaaga
     4321 ttgatttgaa aaactggaaa acgaatcaaa aaaatgctgg attcactcca gaagaaagag
     4381 cggctctttc tcgactgttg gataacgggt ttatcgatac attccgttat ttttatccaa
     4441 cccaggaagg tgtctactct tggtggaact accgattcaa ttcaaggaaa aacaatgctg
     4501 gttggcggat cgattacttt ttgacgagca aagatttaga gccaggacta gctgatgcaa
     4561 agatccatac ggacattatg ggcagtgatc attgtccagt agaattagat ttgaaataaa
     4621 gaggttgtga aaaagtcgtt tagtttcgag tattaagaaa aattttgtga aaatggcttt
     4681 ccacattttg cataaatttg gcataatacc gagaaactgg cttttgaaca ctgtttatcg
     4741 agagattgtg aaaaagctgt cctgcatcaa gtaataagaa cagccaaaca aaaatagttc
     4801 ctcatatttt tcgtttggtt gggcttatta ccgcagatac aagcttttga acaccgttta
     4861 ttaagaggtt gtgaaaaagc tgcactgcat caagtaataa gaacagccaa acaaaaatag
     4921 ttcctcatat ttttcgtttg gttgggctta ttaccgcaga tgcaagcttt tgaacaccgt
     4981 ttattaagag gttgtgaaaa agctgcactg catcaagtaa taagaacagc caaacaaaaa
     5041 tagttcctca tatttttcgt ttggttggac ttattaccgc agatgcaagc ttttgaacac
     5101 cgtttattga gaagttgtga aatcagcgtc ctgacctgag tattagagaa acaaatggca
     5161 aaatagttcc tcatattttg ctattttttg agctaatacc gagggtcagc tgattgaaca
     5221 ccgtttagtg gagaagttgt gaaaaagtcg cctactttcg agtcgtaaga aatttttttg
     5281 aaaatggttc ctcacaactt tgcataaatt tgacttaata ccgagaagtc atgatcccca
     5341 tctaactcca aatcacaagg aatgagttca aaaactagct tttctcaaca aatattacat
     5401 aaaaaattct atgttttttg ttaaatttat ttgctttttt caaaagtatg ttacagtaat
     5461 ttcccaagga ggttttacag aaatgaattt tgttgccatg gattttgaaa ctgccaatca
     5521 tcagccttat agtgcttgtt ctcttgcgct agtcatggta aaaaatagcc agatcgttga
     5581 tgaattctat acattgatac agcctgagac tccttttttc tggcgaaatg tccaaattca
     5641 cggaatccat caagaagacg tacgcaatgc gccgaaattc ccagaagtct ggcaaactat
     5701 ccaaccatat tttcaaaaaa atcgtttagt cgttgcacat aatgctgctt ttgatacaaa
     5761 ggtcttagcg ggatgcttag attattatca tcttgaacag cctaattatc tttctttatg
     5821 tacagtgaaa agtagccgtc gcctgtttcc tgaatttcca aatcatcgat tgaacactgt
     5881 gtgcgaaaat ttgaacatcc aattagatca tcaccatgat gcattggaag atagtcgtgc
     5941 atgtgcggaa atcctgcttt atcaagaaaa aaaattcggg acagatccat tgaaaaaact
     6001 tgtgactata aagtaaaaga atacttatga aacacacaaa gaggtcgtga gcctgccgtt
     6061 tcactccgag tcacaaggag caatttcaaa aaatagcttc tcatgtttat taaatattgt
     6121 gactggtgac cgaggagctg gcaggagaac accgtttatt ggagaggttg tgaaaaagct
     6181 gttctgcatc aagtaataag aacagccaaa caaaaatagc ttctcatatt tttcatttgg
     6241 ttggacttat taccgcagat gcaagctttt ggacaccgtt taccaagagg ttgtgaaatc
     6301 agcgttttga cctgagtatt agagaaacaa acggcaaaat agttcctcat attttgccgt
     6361 tttttgagct aataccgaag gtcagctgat tgaacaccgt ttattcaaaa aaaatggact
     6421 gcgacacgac ttttgtcaca gtcccttcgt gtgtttttct gccttctcaa aaggatgaca
     6481 gtttctcact cttttcctat ttcttctatt caatcaaata actcatttta tatacatcta
     6541 aaaattcacc tagatctgtt cttgcccctc taggcatgac cgcttcgatt tggaagccca
     6601 tttttcggta aaggtgtacg gctctttcat tacgaacttg aacgtcaagc tccaatcgaa
     6661 aaagtacacc catttccttc gcccaactga tgatttcttc tagcataaga gtgcctagtc
     6721 ccattcccca atattcctgc agaatactga taccgacttc tcccacgtga gacaaacgaa
     6781 attgactgtc tgccttgaca gatgctgttc caataatcgt cccttcatag attgcaagca
     6841 gcaataaatt attattgctt tcgtatatat aatccaactc ttcttctaag gtttctggtg
     6901 gtaacaggag ttctgcttca tccaaaacta aaaattctgt ctccttatta acttgtccca
     6961 tcatcaaaag aatgtctttt gcatctcctg gcactgcttc ccgcaagatg atcccaactt
     7021 cttcactcat gattttcagc ccatttttct agaattcgct gtttgaaggt ttcactttgc
     7081 tccccgtatg agattagttt cacataccgc ttttctaaat ctgtatcact tttttctaaa
     7141 atcagttcca aataatcctc cggcagtgct tcttctaata gatttcccca ttcaacaacg
     7201 gaaagcccat caccttcaaa atattcatct aaccctaaat ctgctccgct agctgctacc
     7261 cgataaatat ccatatgata caacggaatc cgaccttgac tatattctct aataatcgtg
     7321 tatgtcggac ttttgatcat ttgcgttatc cctaatcctt gggcaattcc tttcgtcaac
     7381 gttgttttac ccgcacctag atcaccagtc aatatcagat tgtcacctgg cattgcggaa
     7441 tgaccaattg cctctgcgaa ttgttctgtc attgttaatc ctgatagttc gatcattttt
     7501 catcttctcc cgctcttctt tctttttgcc agtatatcgc gtaatgaagt ttttttaaa
//
LOCUS       DACDKM010000117         7466 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20834_2016
            SAMN12640370-rid9891663.denovo.117, whole genome shotgun sequence.
ACCESSION   DACDKM010000117 DACDKM010000000
VERSION     DACDKM010000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640370
            Sequence Read Archive: SRR10040891
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7466)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7466)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742835.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742835.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:04:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,012
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 29 of 75
            Pseudo Genes (incomplete)         :: 33 of 75
            Pseudo Genes (internal stop)      :: 19 of 75
            Pseudo Genes (multiple problems)  :: 5 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7466
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640370-rid9891663.denovo.117"
                     /isolate="E20834_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            285..758
                     /locus_tag="IV334_002417"
     CDS             285..758
                     /locus_tag="IV334_002417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein A"
                     /protein_id="HAP8944446.1"
                     /translation="MQTLAQAKQNFVYQVERIETEEKTKRHLQNIGIVAGSKIVVVNL
                     SGNNAILLVKSHRVAVSREILGQIFVNKKTASSESWTSLDQLKAGETGIVVAIHGQGA
                     VKRRLMDMGLTRNVKIVIRKLAPLGDPIELTVRGYELTLRKSEAELILIQREEKE"
     gene            755..2887
                     /gene="feoB"
                     /locus_tag="IV334_002418"
     CDS             755..2887
                     /gene="feoB"
                     /locus_tag="IV334_002418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HAP8944447.1"
                     /translation="MNIALVGNPNSGKTSLFNLLTGSNQYVGNWPGVTVEKKEGSLKK
                     NPNIHIQDLPGIYSLSPYTPEEVIARDYLVQEQPELIINIIDATNLERNLYLTTQLLD
                     LDIPMIIGLNMMDVIKKEGKKINLEKLSYGLGVPVLPISVLKKQGIEKLIEKIQESAK
                     KRPSIPVPCTYDPRLEAALHEIEDVLGEQSGKKRWYAMKYFERDQKVREDYEIAVSQQ
                     KEIEQLIQLTEKLLDDDSETILVNERYEFITQLCTLCVVSNDSFQLSMSDKIDQIATN
                     RWLALPIFAFVMWLIYYLAIQTVGTMGTDWINDTLFGTWLPEHVGRLLAEWQVAGWMQ
                     ELILNGIIAGVGAVLGFLPQLIILFLCLSFLEDCGYMARIAFVMDRLFRKFGLSGKSF
                     IPMLIATGCGVPGVMASRTIENEQDRRLTIMVTTFMPCSAKLPIIALVAGAFFPHQSW
                     VAPSAYFLGMTAIIFSGISLKKTRLFAGDTAPFIMELPAYHFPQWSAVLRQTYDRSKS
                     FVKKAGTIIFVSSIVIWFSSSYNFYAQPVSEDQSILAFFGRILAVVFQPLGWGNWQGT
                     VAAITGLVAKENIIGTFGILFGHAEVSENGREIWQVLQHTYTPAAAYSLLAFNLLCAP
                     CFAAIGAIHREMNAKKWTWIAIGYQCGLAYLVSFVIYQLGHLLEGGQVALGTYLAILL
                     MIGLVYVLFRQPKSKNQFYTILSLEGEE"
     gene            2889..3023
                     /locus_tag="IV334_002419"
     CDS             2889..3023
                     /locus_tag="IV334_002419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FeoB-associated Cys-rich membrane protein"
                     /protein_id="HAP8944448.1"
                     /translation="MATFLLSVLIFGSAGAIVYTRLKSGKNCDDCQTACPVKKEQIKR
                     "
     gene            3117..3761
                     /locus_tag="IV334_002420"
     CDS             3117..3761
                     /locus_tag="IV334_002420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAP8944449.1"
                     /translation="MSGKKKIVRPRYQQIAVDLAERIVENRYKVGEKIHARSTLASTY
                     NASPETTRKAINVLVDLGIMEVRHGSGAFVASKEKAKDFLLQYQDVQSIQDTKAEIMA
                     SVEKQQDELNHFSQLLDQLVNQTKRIHKMNPLLPFELSLSEKAMHLDRSISELNIWQA
                     TGATVIAISHNEELLVSPGPYAKFTAGDIVHFIGNEYTLQRMTNFFYPEHQLAE"
     gene            3948..5141
                     /locus_tag="IV334_002421"
     CDS             3948..5141
                     /locus_tag="IV334_002421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     ATP-binding protein"
                     /protein_id="HAP8944450.1"
                     /translation="MVKVEVKHLTKIFGKKTQAALDMMNDHQPKTEILKKTGATVGVY
                     DVNFDVKEGEIFVIMGLSGSGKSTLIRLLNRLIEPTSGSIYIDGEDVSKLSKEELREV
                     RRHKINMVFQNFGLFPHRTILENTEYGLEVRGVPKEERQEKAEKALENSSLLSFKDQY
                     PSQLSGGMQQRVGLARALANDPEILLMDEAFSALDPLIRREMQDELLDLQANVQKTII
                     FITHDLNEALRIGDRIALMKDGEIMQIGTGEEILTNPANDYVREFVEEVDRSKVLTAQ
                     NIMVPALTTNIESDGPNVALTRMRNEEVSMLMAVDRKRHLKGIITADQALEARKQKRP
                     LIDFLDENVTVIGKDMVVSDIFNIIYDSPTPLAVVEDGKLKGVVIRGSVIEALAETSE
                     VSEHE"
     gene            5134..6861
                     /locus_tag="IV334_002422"
     CDS             5134..6861
                     /locus_tag="IV334_002422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HAP8944451.1"
                     /translation="MNNLLNYQLPVANWVENITEWFTTTFSGLFSFLQTIGQAVMSGI
                     TNLLLVIPAPLFILLLTVAAFFISKKRPGLTLFTLIGLWFIYNQGLWNDLMNTVTLVL
                     LSSVISIIIGVPLGILMAKSSKAQSIIKPILDFMQTMPGFVYLIPAVAFFGIGMVPGV
                     FASVIFALPPTVRFTNLGIRQVPKELVEASDSFGSTSRQKLFKLELPLAKSTIMAGIN
                     QTTMLSLSMVVIASMIGAPGLGRGVLSALQRAQVGNGFVNGVALVILAIIIDRFTQHL
                     NQPNNKKAAGATTQKSKKRQGLIIGAVVVVILGAIGIGSFSSAKETKRINLSYVEWDT
                     EVASTNVVGEVLKQMGYDVTMTPLDNSIMWKSVSNGESDAMVSAWLPKTHGSQYAQYK
                     DQVEDLGANLTGAKVGLAVPAYMDVNSIDELTDQAGKKIIGIEPGAGVVTAAENTIQK
                     YDNLKDWKVETSSSGAMTVALGQAIKKHEPIVVTGWTPHWMFAKYDLKYLEDPENGMG
                     SEEQIHTMVRKGLKEDQPEAYKVLDNFHWSEKDMEKVMLEINNGKDPQQAAKDWIKEN
                     QELVESWKK"
     gene            7255..7452
                     /locus_tag="IV334_002423"
     CDS             7255..7452
                     /locus_tag="IV334_002423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterocin"
                     /protein_id="HAP8944452.1"
                     /translation="MKHLKILSIKETQLIYGGTTHSGKYYGNGVYCTKNKCTVDWAKA
                     TTCIAGMSIGGFLGGAIPGKC"
BASE COUNT         2435 a         1275 c         1654 g         2102 t
ORIGIN      
        1 gttttgtgtt tttgaaaaaa gagctgaaaa caagtatcgt gtgggaaaat agcaaattta
       61 atagagaaaa aattgtggaa aactatgaag aaatattttt ttctcatttt tatctatcaa
      121 tactaaagat tcgctcataa tatcacaagt ataattgaca ttctcaatta ccgagagtat
      181 tctatgtgag aatgattctc atttatttgc gtaacacgta agaaaattag aacatataat
      241 gaatgatttt aatacatgat ttatttcaca agagaggaat caatatgcag accttagcgc
      301 aggcaaagca gaatttcgtt tatcaagtcg aacgaatcga aacagaagaa aaaacaaaac
      361 gccatttgca aaatatcggg atcgttgccg gaagtaaaat cgtcgtcgtc aatttatcag
      421 gcaacaatgc cattttactg gtaaaaagtc atcgagtggc agtaagtcgg gaaatactag
      481 gtcaaatctt tgtcaataaa aagactgctt cctctgaaag ctggacttct cttgatcaat
      541 taaaagcagg agagactggg atcgtcgtag caatccacgg acaaggcgca gtgaagcgac
      601 ggttaatgga tatgggattg acgcgaaatg taaaaatagt tatccgaaaa ctagcccctc
      661 tgggagatcc aatcgaactg acagtgcgag ggtatgaact gacgttacga aagtcagagg
      721 ccgaactaat cttgatacaa agggaggaaa aagaatgaat atcgcgttag tcggaaatcc
      781 aaacagcggg aaaactagct tgttcaatct cttgactggt tcgaatcaat atgtagggaa
      841 ttggccgggc gtaacagtag aaaaaaaaga aggatcgttg aaaaaaaatc cgaacattca
      901 tattcaggac ctacccggca tctattcttt gtctccttat acaccagaag aagtgattgc
      961 aagagattat ttggttcagg aacagcctga actgatcatc aacattattg atgctaccaa
     1021 tttagaacga aatctttatt taactacgca gttattggat ttagacattc ctatgatcat
     1081 tggtctgaat atgatggatg tcatcaaaaa agaaggaaaa aaaatcaatc tagaaaagct
     1141 ttcctatggt cttggtgtac cagttttacc aatcagcgta ttgaaaaagc aaggaatcga
     1201 aaaactaatc gaaaagattc aggagtctgc aaaaaagcgg ccttctattc ctgttccctg
     1261 tacatatgat ccacgtttag aagcagcttt acatgaaatt gaagatgtac taggagaaca
     1321 aagcggcaag aaacgctggt atgcaatgaa atattttgaa cgagatcaga aggtaagaga
     1381 ggattacgag atagcagttt ctcagcaaaa agagatcgag caactgatcc agttaacaga
     1441 gaaactattg gacgacgata gtgagacgat tctggtcaat gaacgttatg aatttattac
     1501 ccaactatgt acactctgtg ttgtgtcaaa tgattctttc caattatcga tgagtgacaa
     1561 aatcgatcag attgcaacga atcgttggtt agccttgccg atcttcgcat ttgtgatgtg
     1621 gctgatctat tatctggcta ttcaaacagt aggaacaatg gggacagatt ggatcaatga
     1681 cacattgttt ggtacatggt taccagaaca tgttggcaga ttgttagctg aatggcaagt
     1741 agcgggatgg atgcaggagc tgatcttgaa cggcatcatc gcaggcgtgg gagcagtgtt
     1801 aggatttcta ccgcaattga ttatattgtt tttatgcttg agctttctgg aagactgcgg
     1861 atatatggcg aggatcgctt ttgtcatgga tcgacttttc aggaaattcg gtctttcagg
     1921 aaaatccttc attcctatgc tgattgctac aggatgtgga gtgccaggcg tcatggccag
     1981 tcgcacgatt gaaaacgagc aagatcgtcg tttgacgatc atggtcacaa ccttcatgcc
     2041 ttgttctgca aagttgccga ttattgcctt agtagcaggg gccttttttc ctcatcaatc
     2101 ttgggtagct ccttctgcct attttcttgg tatgactgca attattttct ctggaatcag
     2161 cttgaaaaaa acacgtttgt ttgctggaga tacagctcct tttatcatgg aattgcccgc
     2221 atatcatttt cctcaatggt cagcggtcct aagacaaacc tatgaccgca gcaagtcttt
     2281 tgttaaaaaa gcaggaacga ttatctttgt ttctagtatc gtgatctggt tcagctcttc
     2341 ttataatttt tatgctcaac ctgtttctga agaccaaagt atcttagcct ttttcgggcg
     2401 aatcctggca gttgtttttc agccgcttgg ttggggaaac tggcaaggaa cagtcgcagc
     2461 gattacagga ttggtcgcta aagaaaatat tattggcaca tttggtattt tgtttggtca
     2521 tgcggaagta tccgaaaatg gcagagaaat ctggcaagtg ctacaacaca catatactcc
     2581 cgcagcagct tattcattgt tagccttcaa tttactttgt gcgccttgtt ttgcggcaat
     2641 tggggctatc catcgggaaa tgaatgccaa gaaatggact tggatcgcca taggctatca
     2701 atgcggttta gcttatttag ttagttttgt gatttatcag ttgggacact tattggaggg
     2761 tggacaggta gcgttaggga cgtatcttgc tattttactg atgataggac tcgtttatgt
     2821 tttgttccgt cagccaaaat ccaagaacca gttttatact atattgtcat tagaaggaga
     2881 ggaataggat ggcgacattc ttattaagtg tgttaatctt tggaagtgca ggcgcaattg
     2941 tctatactcg tttaaaatcc ggtaagaatt gtgatgactg tcaaacagct tgcccagtaa
     3001 aaaaggaaca gatcaagcga tgatcagtcg ttgcaagagg ctattctccc cttttttgct
     3061 tattttgcta gagcatagta agatgaggga aagtaaaaaa agaaagagga aagaaaatgt
     3121 ctggaaagaa aaaaatcgtc cgcccgcgct accagcagat tgctgttgat ttagccgaac
     3181 gtatcgttga aaaccgatat aaagtgggtg agaagatcca tgcccgctca acacttgcca
     3241 gtacgtacaa tgcttctcct gagactaccc gcaaggctat caatgtgctg gtagatcttg
     3301 gaatcatgga agtacggcat ggaagtgggg catttgttgc ttcgaaggaa aaagcaaaag
     3361 atttcctatt gcaatatcaa gatgtacaat ccatccaaga tacaaaagca gaaatcatgg
     3421 caagtgtgga gaagcaacaa gatgagctga atcatttttc tcaattattg gatcagctgg
     3481 tcaatcaaac gaagcgaata cataagatga atcctttact tccgtttgaa ttatctttat
     3541 cagaaaaagc aatgcatctg gaccgttcaa ttagcgagtt gaatatctgg caggcgactg
     3601 gcgccacagt tatcgcaatt tctcataatg aagaactgct tgtttcacct ggcccctatg
     3661 ctaaatttac ggcaggggac atcgttcatt ttattggaaa tgaatataca ctacagcgta
     3721 tgacaaactt tttttacccg gagcatcagc ttgcagaatg attgtgtgaa atattaaaaa
     3781 aatattaact tggctaaacc ttttatttac aaagatttag ccaagttttt ttcagtttgg
     3841 acaagcaaat ttctttcatt tttgacaaag tgactacatg atgttaacat agtgtggtca
     3901 cttttttcag aaagaaaaat gaatttacat aaaaaaggag aatttgtttg gtaaaagtag
     3961 aagtaaaaca tttaacaaag atatttggta aaaagacaca agccgcttta gatatgatga
     4021 atgatcacca gccgaaaacg gagattttaa agaaaaccgg tgcaacggtt ggtgtgtatg
     4081 acgtgaattt tgacgtaaaa gaaggagaaa tcttcgtgat catggggctt tccgggagtg
     4141 ggaaatccac attgatccgt ctattgaatc gtctgattga gccaacttcg ggcagtatct
     4201 atattgatgg agaagatgta tcgaagttaa gcaaagaaga attgcgagaa gtccgtcgtc
     4261 ataaaatcaa tatggttttc caaaacttcg gtctgtttcc acatcgaacg atcttggaaa
     4321 atacagaata cgggttagaa gtccgtggtg tacctaaaga agaacgacaa gaaaaagcag
     4381 aaaaagcatt agaaaattct agtttgcttt catttaaaga ccaatatcct agtcagttat
     4441 ctggtgggat gcaacaacgg gttggcctag ccagagcttt agcaaatgat cctgagatct
     4501 tgctgatgga cgaagcgttc tcggctcttg atccgctgat ccggcgagaa atgcaagacg
     4561 aattactgga cctgcaagcc aatgttcaaa aaacgattat tttcattacc catgatttga
     4621 atgaagcttt acggattggg gacagaatcg ccttgatgaa agacggcgaa atcatgcaga
     4681 tcggaacagg tgaagaaata ttgaccaatc cagcaaatga ttatgtacgt gaatttgtgg
     4741 aagaagtcga tcgttccaaa gtgcttacgg ctcaaaacat catggtacct gcattgacga
     4801 cgaatatcga atctgatgga ccaaacgttg cattgacaag gatgcgtaat gaagaagtca
     4861 gcatgctgat ggctgttgac agaaagagac acctgaaagg gatcatcact gcagatcaag
     4921 cgttagaagc taggaagcaa aaacgtccat tgatcgactt cctagatgaa aatgtgacgg
     4981 tgattgggaa agacatggtg gtaagtgata ttttcaatat tatttatgac tcaccgactc
     5041 cacttgcagt tgtagaggat ggaaaattaa aaggtgtcgt catccgaggt agtgtgattg
     5101 aagcattagc agaaacaagc gaggtgagtg aacatgaata atctattgaa ctatcaatta
     5161 ccagtggcaa actgggtaga aaatatcaca gaatggttta cgacaacttt ttctggccta
     5221 ttttcatttt tacaaacgat tggacaggca gtgatgtcgg ggattacaaa tttactgtta
     5281 gtgattccag ctcctttgtt tatcttgtta ttgacggtag cagctttctt tatttctaag
     5341 aaacgtccag gacttacgtt gttcacatta atcggtctat ggtttattta taaccaaggt
     5401 ctatggaatg atttgatgaa tactgtcaca cttgttcttt tatctagtgt gatctcgatc
     5461 atcattgggg taccattagg gatcttgatg gcaaaaagca gtaaagccca aagtattatc
     5521 aaaccgatcc ttgactttat gcagacaatg ccaggattcg tttatttgat tccagcagtt
     5581 gcatttttcg gtatcgggat ggttccaggg gtatttgcct cagtcatctt tgctctgcca
     5641 ccaaccgttc gttttacgaa tttagggatt cgacaagttc ctaaagaatt agtagaagct
     5701 tctgattcat tcgggagcac aagtcgtcaa aaactattca aactagaatt gcctttagct
     5761 aaaagtacga tcatggcagg gatcaatcaa acaacaatgc tgtcactttc aatggtcgtt
     5821 attgcctcta tgatcggtgc acccggctta ggacgcggcg tattatcagc attgcaaaga
     5881 gcacaagtcg ggaatggatt tgtcaacggg gttgcattag ttatcttagc aatcatcatt
     5941 gaccgtttta cacagcattt gaaccagcca aacaataaaa aagctgcagg agcaacaaca
     6001 cagaaaagta aaaaacgtca aggactgatc attggtgctg tagtagtagt gatcttaggt
     6061 gcgattggta tagggagttt ttcatctgcc aaagaaacaa aacgtatcaa cttatcttat
     6121 gttgaatggg atacagaagt tgcttcaaca aatgtagtcg gtgaagtatt gaaacaaatg
     6181 ggctatgatg taacgatgac gccattagat aattcaatca tgtggaaatc tgtttcaaat
     6241 ggcgagtcag atgccatggt ttcagcttgg ctacctaaaa ctcatggttc acaatatgca
     6301 caatataaag atcaagtcga agacctaggt gccaatttga caggagcaaa agttggttta
     6361 gcagtgccag cctacatgga tgtaaattcg atcgatgagt taaccgatca ggcaggtaag
     6421 aaaatcatag gaatcgagcc aggagctggt gtagttacgg ctgctgaaaa cacgatccaa
     6481 aaatatgaca acttgaaaga ctggaaagta gagacatctt cttcaggagc aatgaccgtt
     6541 gcactaggtc aagcgatcaa aaaacacgaa ccaattgtag ttacaggatg gacacctcat
     6601 tggatgtttg ccaaatatga tctgaaatac ttagaagatc ctgaaaacgg aatgggaagc
     6661 gaagagcaga tccatacgat ggttcgaaaa ggattgaaag aagaccagcc agaagcttac
     6721 aaagtattag acaatttcca ttggtcagaa aaagatatgg aaaaagttat gttggaaatc
     6781 aacaatggaa aagatccaca gcaagcagca aaagactgga tcaaagagaa ccaagaatta
     6841 gtcgaaagct ggaaaaaata aaaagacaga cacgaaataa atggagcttc caaaattgga
     6901 tcaaatatcc aaatttggaa gctccatgtt cgtctctatc ttttttgtct ataaaaaaag
     6961 agaatgcgta gctcatcttc gaaacgtcca aagacgccga caaactatta tgaatttatg
     7021 ggtgcgagtc aaagagatcg atcgtaggta tgaaagacta atgtgattaa agtttattat
     7081 gtacgaagtg cattctcaaa aaatattaaa gaatatacat agtttatata cttaaaagtg
     7141 attaaattct tattcaggaa ttattttgct acgtttcaag aaggaattgt tttttgaaat
     7201 ttaatgaatg ttagaataat agtctaaaaa cattaattta ggggtgatta gattatgaaa
     7261 catttaaaaa ttttgtctat taaagagaca caacttatct atgggggtac cactcatagt
     7321 ggaaaatatt atggaaatgg agtgtattgc actaaaaata aatgtacggt cgattgggcc
     7381 aaggcaacta cttgtattgc aggaatgtct ataggtggtt ttttaggtgg agcaattcca
     7441 gggaagtgct aaaatgaaaa aaaatg
//
LOCUS       DACDKM010000118         7111 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20834_2016
            SAMN12640370-rid9891663.denovo.118, whole genome shotgun sequence.
ACCESSION   DACDKM010000118 DACDKM010000000
VERSION     DACDKM010000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640370
            Sequence Read Archive: SRR10040891
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7111)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7111)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742835.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742835.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:04:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,012
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 29 of 75
            Pseudo Genes (incomplete)         :: 33 of 75
            Pseudo Genes (internal stop)      :: 19 of 75
            Pseudo Genes (multiple problems)  :: 5 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7111
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640370-rid9891663.denovo.118"
                     /isolate="E20834_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            6..689
                     /locus_tag="IV334_002424"
     CDS             6..689
                     /locus_tag="IV334_002424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage regulatory protein"
                     /protein_id="HAP8944453.1"
                     /translation="MLKNKEAVTTSLQVAESFEKKHQHVLRDIDALKKDVSNFGQMFV
                     EGNEPDSYGRNRRVFFISRDGFFLLAMGFTGKKAIYFKQKYIEAFNEMEDVIRKNTVP
                     QTIEDMMIYQLEEMKDVKKDVSMLKDTMRISGQQEFEIKQKGNMKVMGVLGGKESRAY
                     EEISKKVFSKFWSEFKRTFSIPRYGELPRKRFDDAVSFIEMWLPETAIRMEIDQLNRQ
                     QRLFGDDNE"
     gene            867..1208
                     /locus_tag="IV334_002425"
     CDS             867..1208
                     /locus_tag="IV334_002425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8944454.1"
                     /translation="MEKELSTLDQYLIDPDWGKPKIEETSGRKIRRNLLTNEELAWDQ
                     DDLGNHVTIWDHVYLIHLSKHSNKPEYIYVIEDGLIDALEEYDRDNLIDISYYGPGKK
                     YIAEMEAEFDE"
     gene            1201..1872
                     /locus_tag="IV334_002426"
     CDS             1201..1872
                     /locus_tag="IV334_002426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1071 domain-containing protein"
                     /protein_id="HAP8944455.1"
                     /translation="MSEIKGTTNFEKLFSRKLNKILKKKGNFDYLSWAHAWEIMKKND
                     PQATVTINEYKHYRVVSGTHQDFLVEEYKPFLMDETGTYVSVSVTVKGHTETELFPVL
                     DYRNQPVVKPNAMQINNSLKRCFVKALALHGLGLYVFQGEDIPTPPRIDTKKLNMLET
                     ILEAFNEQMGKDMTKTLIEYVNEQTDKLGLLADNVETIEQLSYEQCALMERAIAAKRK
                     ELDKK"
     gene            1878..2564
                     /locus_tag="IV334_002427"
     CDS             1878..2564
                     /locus_tag="IV334_002427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8944456.1"
                     /translation="MFKPLIDSYSAVLKKFKGKDISATINEEVNIDRLKTMYDGYDGD
                     RVIEIRFIDPRRFTVQQRNFIYALIGDIFIDTGMPTDFWKEFFYFRFEGVTGREISLK
                     DESNTTVSDANILANIILDFIFEHHIPFKEGYEILPANQEYYFYKCITKRVCCICGKT
                     GADIDHFDKALGRRKRKEVDHAEYTFAALCRIHHTEKHKIGVINFKNKYQIKGIKLNQ
                     KTIKKLNIGG"
     gene            2567..3316
                     /locus_tag="IV334_002428"
     CDS             2567..3316
                     /locus_tag="IV334_002428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP8944457.1"
                     /translation="MTEHRSYYAIIPANVRYDKRLKPNTKLLYGEITALCNERGFCWA
                     GNEYFADLYGVNKETISRWVSDLIKFGYLNREIIYKEGTNQIINRYLRINQYPIDEKR
                     NTPIDEKVKDNNTSINNTFNNTKEYIRELPPSKKSKAKPVRHKYGEYKNVLLSDEQME
                     KLKTEFPNDYQERIERLSEYCESSGKTYKNYLATIRSWARKEKSEPKNASSGYKRTGR
                     REKLPEWAIDQEAYLKKKALERANRQSKAPF"
     gene            3328..3597
                     /locus_tag="IV334_002429"
     CDS             3328..3597
                     /locus_tag="IV334_002429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8944458.1"
                     /translation="MKIDYLELINEIANYKKGEELDVLRDVYDQLEEAGIEGIKNDRS
                     SWSKLRYYFALYIDTTQLRNLAYTKLLFVDCVKGLQKHLNELEQV"
     gene            3759..4061
                     /locus_tag="IV334_002430"
     CDS             3759..4061
                     /locus_tag="IV334_002430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8944459.1"
                     /translation="MDKKAAMQRIIELTYSEDWQNDKEAASEVMRLGRAMWADKSNKP
                     RPRKIAIWHGDKLLVIGTAEQLASLTGLHEKIVRKRARCGYTDAKKRTFRYVEESS"
     gene            4058..4219
                     /locus_tag="IV334_002431"
     CDS             4058..4219
                     /locus_tag="IV334_002431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HAP8944460.1"
                     /translation="MTTEEVIQMRIRSLQREIDDLERTKAVMVNETARKAIDLHIENL
                     RREIHRLEE"
     gene            4216..4521
                     /locus_tag="IV334_002432"
     CDS             4216..4521
                     /locus_tag="IV334_002432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8944461.1"
                     /translation="MSVDKKAAMKRIAELTKSESWQEDKEIVAEVQKLGKSMWTEKPK
                     RRTPRKIAIWHDDRILVTGTAEQLSEITGLSKNIIWDRARSLWIDSKGRQFRYVEER"
     gene            4521..4877
                     /locus_tag="IV334_002433"
     CDS             4521..4877
                     /locus_tag="IV334_002433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1140 family protein"
                     /protein_id="HAP8944462.1"
                     /translation="MDLITQYSDIILKKIMMKIQKDKKSKERAELVKLEMAETGAGVR
                     SSRHWKAAANIEFYYNEIQKGFDQMRELDRQTNWSKKLHQDRFKFVEKYKEILEEYLR
                     RTANDKKTRSIQHGFI"
     gene            4837..5082
                     /locus_tag="IV334_002434"
     CDS             4837..5082
                     /locus_tag="IV334_002434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8944463.1"
                     /translation="MIKKLVQFSMDLYDIESGATLSVESDHLIINFGGKRQIILWVVD
                     DVLFPEIVHDFEESKAVEFEIVKKVMELIEKYEEDSE"
     gene            5079..5498
                     /locus_tag="IV334_002435"
     CDS             5079..5498
                     /locus_tag="IV334_002435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-packaging protein"
                     /protein_id="HAP8944464.1"
                     /translation="MIPKFRAWDKRKNVMRDVAVLHFTKNGKTNFIEYWINPTELKSY
                     HVRNIDLMQSTGMKDKNGVDIFEGDIVLVSVRNGFDYLDNKVCIVKNSIDYSGLVCAT
                     VDEDLEYQIFNTELFEEYTYEVTGNIYENSELLEEQR"
     gene            5495..6052
                     /locus_tag="IV334_002436"
     CDS             5495..6052
                     /locus_tag="IV334_002436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1642 domain-containing protein"
                     /protein_id="HAP8944465.1"
                     /translation="MNKQEAIKKLESIKAIGNDAIAACYNESINSGITLMKKIDEPQK
                     PVIPQLVAGWLEKSTDPFTKAEKIAYLIKSKDGDSYYFCDWFVRDGIVTQEQGEELLA
                     WATRQSYETLLSLYNGYEVEKEPLWAIKNADGNYLTKCALWGKDGVNYSFECNPSHRL
                     LFTDKATADAAALLVNGTVEEVVER"
     gene            6049..6345
                     /locus_tag="IV334_002437"
     CDS             6049..6345
                     /locus_tag="IV334_002437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8944466.1"
                     /translation="MKLKDGFYASSHGIGGLMLDMPTKNPKTRKKSKFKVGDMVRCEA
                     EEFVYPFRGYVKKILSNSAIIRIENTMKCDRSTAKHKHYLAVARLVDMEVIKSK"
     gene            6422..6835
                     /locus_tag="IV334_002438"
     CDS             6422..6835
                     /locus_tag="IV334_002438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autolysin"
                     /protein_id="HAP8944467.1"
                     /translation="MQLLREVDFKQTRCNARDVLKNFRRLERMAGRSLIDIKSPIITD
                     MPKAPKHGNKAEDAIIQMMDIEAERDAILAALMALSLISRQILYYSFCVPDSFSNYRI
                     SREVGYSERSIQRMKSEALIEFAEAYKHGRIIAYK"
BASE COUNT         2629 a         1105 c         1586 g         1791 t
ORIGIN      
        1 tagtaatttt gaaaaataaa gaagctgtga ctacgagctt acaagtggca gaaagcttcg
       61 agaaaaaaca tcaacatgtt ttaagagata ttgatgcact aaaaaaagat gtgtccaatt
      121 ttggacagat gtttgtagaa ggtaatgaac cagattcata tggcagaaat cgacgagttt
      181 tcttcattag tagagatgga tttttcttgc tggctatggg gtttacagga aagaaagcta
      241 tctacttcaa acaaaaatac attgaagcat tcaacgaaat ggaagatgtt attcgcaaga
      301 atactgttcc tcaaacaatt gaagatatga tgatctatca attagaagaa atgaaagatg
      361 ttaaaaaaga tgtttccatg cttaaagata ctatgcgaat tagcggacaa caagagtttg
      421 aaattaagca aaaaggaaat atgaaagtta tgggagttct agggggaaaa gaaagccgag
      481 cttatgaaga aatcagcaaa aaagtattct caaaattttg gtctgaattt aaacgtacct
      541 tttcaatccc aagatatggc gagttacctc gtaagagatt cgatgatgct gtttcattta
      601 ttgaaatgtg gttgccagaa actgcgatcc gtatggaaat tgatcaactg aacagacaac
      661 agagactttt tggtgatgac aatgaataga gctgaagcgc taagaatagg gacggcaatt
      721 gctaattgct ggtggaaata ctataaacca atcatcctaa gccaacaaca tattgacaag
      781 caaaaagcat ggcaacaaat aaaaaagtga ctccgccggc aagcaaagag tcacaaagaa
      841 aacacatcat aaggagattt tagcatatgg aaaaagaact ttccactcta gatcaatatt
      901 tgattgatcc tgattggggc aagccgaaaa ttgaggaaac aagtggtcga aaaatcagac
      961 gaaatctttt gacgaatgaa gaactagctt gggatcaaga tgatttaggc aaccatgtaa
     1021 ctatttggga tcatgtttat cttatccatc tatcgaagca ttcgaataaa cctgaatata
     1081 tttacgtcat cgaagatggc ttgattgatg cgctagaaga gtacgacaga gataacttga
     1141 ttgatatctc ttattacgga ccaggtaaga aatacattgc tgaaatggag gcagaatttg
     1201 atgagtgaaa tcaaagggac aacgaacttt gaaaaacttt ttagtcgtaa gttaaataaa
     1261 attctcaaga aaaaaggaaa ttttgattat ttatcttggg ctcacgcgtg ggagattatg
     1321 aaaaagaatg atccacaggc aacggtaact attaatgagt acaaacacta cagggttgtt
     1381 tctggaactc atcaagactt tcttgttgag gaatataaac cttttcttat ggatgaaact
     1441 gggacttatg tatctgtctc agtaacggtt aaaggacaca cggaaaccga attatttcct
     1501 gttttagatt atcgaaacca accagttgtt aaaccaaatg caatgcaaat caataactca
     1561 ttgaagcgat gctttgtgaa agcattggct ctacacggac tgggattata tgtatttcaa
     1621 ggggaagata ttccaacacc acctagaatc gatacaaaga aattaaacat gctagagacg
     1681 attctagaag ctttcaatga gcagatgggt aaagatatga ccaaaacctt aatcgaatat
     1741 gttaatgagc agacagataa attagggctc ttagctgata acgttgaaac tattgaacag
     1801 ttaagctatg agcaatgtgc cttgatggag cgagcaatag cagctaagag aaaagaatta
     1861 gataagaagt gatatgagtg tttaaaccat taatcgattc atattcagcg gttctgaaaa
     1921 agttcaaagg aaaagacata agcgcaacca tcaatgagga agtgaacatt gatcgactaa
     1981 agacgatgta tgacggctac gatggtgatc gagtcattga aattcgtttt attgatccta
     2041 gacgtttcac cgtacagcaa cgaaacttca tctatgcgct gataggcgat atttttatcg
     2101 atacaggcat gccaacggac ttctggaagg aattcttcta cttccgtttt gaaggtgtca
     2161 cagggcgcga aataagcctc aaagacgaat cgaatacaac cgtgagtgat gccaatatct
     2221 tagcgaatat catcctagat ttcatctttg aacatcatat tcctttcaaa gaaggatatg
     2281 agattttacc agcgaatcaa gagtattact tctacaaatg cattacaaaa agagtctgct
     2341 gcatctgtgg caaaacagga gctgacatcg atcactttga caaagcgcta ggaagacgaa
     2401 agcgcaaaga agttgatcat gcagagtaca catttgcagc actctgcaga atccatcaca
     2461 cagagaagca caaaataggt gtgattaatt tcaaaaataa atatcaaatc aaagggatca
     2521 aattaaacca gaaaacaatc aaaaagttaa atattggagg gtaaaaatga cagaacatcg
     2581 aagttattat gcgattatac cagccaacgt aaggtacgac aaaagactta aaccaaatac
     2641 taagttgtta tacggagaga taacggcctt gtgtaatgaa agaggctttt gttgggcagg
     2701 caatgagtac tttgcagatt tatatggtgt gaataaagag accatatcgc gatgggtaag
     2761 tgatttgatt aagtttggat acttgaatcg ggaaatcatt tacaaagagg gtaccaatca
     2821 aataatcaat aggtacctac gaattaatca ataccctatt gacgaaaaac gcaatacccc
     2881 tattgacgaa aaagtcaaag ataataatac atctattaat aatacattta ataatacaaa
     2941 agaatatata agagagttac cgccttcgaa aaaatcgaag gctaagcccg tccgtcataa
     3001 atacggagag tataaaaatg ttcttttgtc agatgagcag atggagaaac tcaaaacaga
     3061 attccctaat gattaccaag agcgaatcga acgactgtca gagtattgtg aatcatctgg
     3121 taagacttat aaaaactatt tggcaactat tcgaagttgg gcaaggaaag aaaaaagtga
     3181 acctaagaac gcaagcagtg gatacaagcg cacaggaaga cgagagaagc ttcctgaatg
     3241 ggcaatcgac caagaagcct atctcaagaa aaaagcgcta gaacgagcta atagacaatc
     3301 aaaagcacca ttctaagagg tggaaaaatg aagatcgatt atctagaact aattaatgaa
     3361 atagcgaatt ataaaaaggg cgaggaatta gacgtcctga gagacgtata tgatcaactc
     3421 gaagaagctg gaattgaagg aattaagaat gatcgttcga gttggagtaa gctcagatac
     3481 tatttcgcac tttatatcga tacaacacaa ttaagaaatt tagcatatac caaattacta
     3541 tttgttgatt gtgttaaagg cttgcaaaaa catcttaatg aacttgagca ggtgtaatca
     3601 gatggaccta aagacattta cagcacagat tgaactaatg catcaagaag ctttaagaca
     3661 aagtgtatcg tacgaagaca agtggctcaa cacgttccat ggcggacgtg agagcgcact
     3721 tgatcaagta ctcaaattac tgaaaggaga atgtcgggat ggataagaaa gcggcaatgc
     3781 agcgaattat cgaattgact tattcagaag attggcaaaa tgacaaagaa gctgcttcag
     3841 aagtgatgag gcttggaaga gcgatgtggg cagacaagag caacaagcca agaccacgaa
     3901 aaatcgcaat ttggcacggt gacaaacttc tagtgatagg gacagctgaa cagttagcaa
     3961 gtctcacagg cttgcacgag aaaatcgtga ggaaaagagc aaggtgtggc tacacagacg
     4021 ctaagaagag aacgtttaga tacgtggagg aatcatcatg acaacagaag aagtgattca
     4081 aatgcgtatt cgaagccttc agcgtgagat tgacgatctg gaacgaacaa aggcagtgat
     4141 ggtcaatgaa acggcgagaa aggcaatcga tttgcacata gagaatttaa gaagggaaat
     4201 ccatcgattg gaggaatgag cgtggataag aaagcagcaa tgaaaagaat tgctgaatta
     4261 accaagtcag aatcttggca agaagacaaa gaaatagttg cagaagtcca aaagctcggt
     4321 aaatcaatgt ggactgaaaa gcccaaacgg agaacgccga gaaaaattgc aatctggcat
     4381 gatgaccgaa ttttagtaac aggtactgct gaacagttat ctgaaattac tggattaagc
     4441 aaaaacatta tctgggatag agctaggagc ttatggattg attcaaaagg acgacagttt
     4501 aggtatgtgg aggagagata atggatctca ttacacaata cagtgacatc atcctcaaga
     4561 aaatcatgat gaagattcag aaagataaaa aatcaaaaga acgagctgaa ttagttaagt
     4621 tagaaatggc tgaaacagga gcaggagtgc gaagtagcag gcattggaaa gcagcagcaa
     4681 acattgaatt ttattacaac gaaattcaaa aagggttcga tcagatgcgt gagctggatc
     4741 ggcaaacaaa ttggagcaag aaacttcatc aagatcgttt caaatttgta gagaagtata
     4801 aagaaatatt agaagagtat ttgaggagga cagcaaatga taaaaaaact cgttcaattc
     4861 agcatggatt tatatgatat cgaatcagga gcaacactat ctgtggaatc ggaccatcta
     4921 atcataaatt ttggtggaaa gcgccagatt attttgtggg tagttgatga tgtactgttt
     4981 ccagaaattg ttcatgattt cgaagaatca aaagcggttg agtttgaaat agtgaaaaaa
     5041 gtaatggaat tgattgaaaa atacgaggag gacagcgaat gataccgaag tttagagcgt
     5101 gggataaacg aaagaacgta atgagagatg tagccgtctt gcattttact aaaaacggca
     5161 aaacaaactt tattgaatat tggataaatc ctaccgaatt gaaatcatat cacgtgcgaa
     5221 acatcgacct catgcaatcc acaggaatga aagataagaa tggtgtggat atatttgaag
     5281 gggatatagt attagtcagc gtgcgaaatg gcttcgatta cttagataat aaagtctgta
     5341 ttgtcaaaaa ttcaatagat tattccggat tagtttgtgc cactgttgat gaagacttag
     5401 agtatcaaat ttttaacaca gagctgtttg aagaatacac gtacgaagtc accggaaata
     5461 tatacgagaa tagcgagtta ttggaggaac agcgatgaat aaacaggaag caatcaaaaa
     5521 gttagaaagt attaaagcga taggaaatga tgcaatagct gcttgctata acgagagtat
     5581 aaattcaggt attacgttaa tgaaaaaaat agacgaaccg cagaaaccag ttattccaca
     5641 actcgtggcc ggttggcttg agaaatctac ggaccctttt acaaaagctg aaaaaatagc
     5701 gtatttaatc aaatctaaag atggtgattc atattatttc tgtgattggt ttgtacgaga
     5761 tggcatagtg acgcaagagc aaggagaaga attactcgct tgggcaacaa gacaatcata
     5821 tgaaacacta ttgagcctat acaacggcta cgaggttgag aaagagccgt tatgggcaat
     5881 aaagaatgcc gatggaaact atcttactaa atgtgcttta tggggaaaag atggagtaaa
     5941 ttatagtttt gaatgcaatc catctcatcg attgcttttc actgataaag caacagcgga
     6001 tgctgcagca ttgttggtga atggaacagt ggaagaggtg gtagaaagat gaaactaaaa
     6061 gacggatttt acgctagcag tcacggtatc ggcggtttaa tgctagatat gccgacaaag
     6121 aaccctaaaa cacgcaagaa atcaaaattc aaagtcggtg acatggtccg ctgtgaagca
     6181 gaagagttcg tttatccgtt cagaggatac gttaaaaaga tactgtcaaa ctcagcaatc
     6241 attcgtattg aaaatacaat gaagtgtgat cgttcgactg caaaacacaa gcattattta
     6301 gctgtagcga gattagttga catggaagta atcaagagca aataaaaaaa gccggatcgc
     6361 tccgactgat tcaataaatc caacacattt attatatcac ataaaggagc ggtttgactt
     6421 gatgcaattg ttacgagagg tagatttcaa acagacaaga tgtaatgcga gagatgtgct
     6481 gaagaacttt cggcgtttgg agcggatggc aggtcgctct ttgatagata ttaagtcgcc
     6541 gattattacg gatatgccga aggcaccaaa gcacggcaat aaggcagaag acgcgatcat
     6601 tcagatgatg gatatagaag cggagagaga tgcgattcta gcggctttga tggctcttag
     6661 tctaattagt cgccagatac tctactacag cttctgtgtg ccagatagct tctcaaacta
     6721 caggattagc cgtgaagtgg gttattcaga aagaagtata caacggatga agtcggaagc
     6781 tctaatagag tttgcagaag catataaaca cggaagaata attgcttata aataatttgg
     6841 cggttttttg gcggaatgat ggcggttttt agctatttac cagtgatatt atggtagtgt
     6901 cgaaagatta gtgataggtc taagacaaaa taaaatgtaa gggaggaaat ctccctcatc
     6961 gtttaattaa gcttcgatag acagcagcaa ataaactaaa ggatgtgggg gtcagctcct
     7021 acagttagtt catgtgttgc tgtctattaa ttgcaactct ttcggtttta ttgagtattt
     7081 actcaactta aaaaaactgt tgtttgtaga t
//
LOCUS       DACDKM010000119         6654 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20834_2016
            SAMN12640370-rid9891663.denovo.119, whole genome shotgun sequence.
ACCESSION   DACDKM010000119 DACDKM010000000
VERSION     DACDKM010000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640370
            Sequence Read Archive: SRR10040891
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6654)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6654)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742835.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742835.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:04:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,012
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 29 of 75
            Pseudo Genes (incomplete)         :: 33 of 75
            Pseudo Genes (internal stop)      :: 19 of 75
            Pseudo Genes (multiple problems)  :: 5 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6654
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640370-rid9891663.denovo.119"
                     /isolate="E20834_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(<1..262)
                     /locus_tag="IV334_002439"
     CDS             complement(<1..262)
                     /locus_tag="IV334_002439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4064 domain-containing protein"
                     /protein_id="HAP8944468.1"
                     /translation="MNKRKTEFILILISGITGILCSLLALMGGMFLSSIDSTTLNTTE
                     LTSKDIDMLVNLSGNVVLFGGITLVFSIMIFIIGFLVRKNQKT"
     gene            complement(259..780)
                     /locus_tag="IV334_002440"
     CDS             complement(259..780)
                     /locus_tag="IV334_002440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HAP8944469.1"
                     /translation="MNKKSGIETLPNYVFAGFWLRFFAFLIDLICISIITHATIGLFY
                     KIMGLESDKSIITFYGIASLAIYLTYFVLLTKLNSGQTIGKMIFGIRVVSFEEKELSW
                     QTIFIRECICRFILKNFVFQIGYLVAAFTPKKQHIGDYFSNTSVVVIRSLEAINYGQK
                     ISNSKTLNLERIK"
     gene            complement(782..1816)
                     /gene="sppA"
                     /locus_tag="IV334_002441"
     CDS             complement(782..1816)
                     /gene="sppA"
                     /locus_tag="IV334_002441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HAP8944470.1"
                     /translation="MNKRRWIAVGVACGLLLLSIIVALIPGKDKEEASNTTLTGINKI
                     FYGSNEITEETLEEGASNKKIVKLSVNGVIADTGESNLFSREQYNHQNFLTQLKKIQE
                     DKAVKGVLLEVNSPGGGIYESAEIAKEMAKIKKLDIPIYTAFKNTAASGGYYISAGSD
                     KIFATEETTTGSIGVIISGLNYSGLLEKLGVTDATYKSGALKDMMSPQHKPSEEENKV
                     IQEFVMSAYDRFVNVVAKGRNMDTNAVKELADGRIYDGNQAVENGLVDQIGYSEDALD
                     SLKKEKKLTDATVIEYKNDTTGFASSWLGNKIAEWQGLKATTSDRVISVFEKLGFSDT
                     PKPMYYYGGL"
     gene            complement(1849..3264)
                     /locus_tag="IV334_002442"
     CDS             complement(1849..3264)
                     /locus_tag="IV334_002442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HAP8944471.1"
                     /translation="MVSIKNYGKSRRGKQFMQNNNWFEHSSIYTKQHHSFYRWVLYPV
                     ILFLCIIGLFLTFAKKEVVIRTTAQLNPEKIEKLQVPLEAKIMENFLKENQFVHKDDV
                     LVRLDCSLIENEKAQIEQENQRITQQIKMAQLFIESISKGKNLFSTDDSFGYSNQLKS
                     MLSEKESLRYALKQSELNDQKQLEVYEKTKRQLEKQIESSDSKLQEWQQVQVAWSNNQ
                     SLKDFSKEMMANYENWQEQLNNVSDDQKNQVKLTISASINEQIEQLKKEVEQYQSEKA
                     KLVKPTTSENDRISQTEKGKQELEQAITTVKQTVTELNEKQEKNQSVIKSLDDQLSKG
                     ILKAPVSGTVHLNEETKGQAELAKGTVLAEIYPIHEKSKMKFTALLPANESTYIKEGM
                     KVHFKLDQKGGAPITIDGTLDEISENSTATEKGVFYAVKGTLKTTQSFPFRYGLTGEL
                     SLIVGEKTYWQQIVDVFLNKK"
     gene            complement(3233..5386)
                     /locus_tag="IV334_002443"
     CDS             complement(3233..5386)
                     /locus_tag="IV334_002443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide cleavage/export ABC transporter"
                     /protein_id="HAP8944472.1"
                     /translation="MIKSFPFVQQQDEKDCGVACLSMILKYFKTDIPIHKLRDLSGTD
                     LEGTSAFGLKKTFEKLSFDCMAIQADSEIWKERDLILPLIAHILVDNSYMHYVVIYKT
                     KGNVLWIADPANGKNRKTIEAFQKEWTGILLLPMPRETYIPIKEKVAGLSSFLPIIWK
                     QKGLVFHIVLASIFITFFGIGSSYYFQGILDFFIPNQARSTLNIISIGLIAVYLFRVL
                     FEYSRSYLLIILGQRMSMAIMLQYFKHVLTLPMNFFATRKSGEIISRFLDASKIIDAL
                     ASATLSIFLDIGMVLLVGVTLAIQNSTLFLITLCSLPFYVVAIFAFVKSYEKSNREEM
                     AAGAAVNSSIIESLKGIETIKAYSGEEKVYNRIDHEFVKLMKKSFRTVTLDNIQQGIK
                     HLIQLISSALILWLGSYFVMDNRISLGQLITYNALLVFLTDPLQNIINLQVKMQTAQV
                     ANNRLNEIFAIDSEKSNQSFEYMLSEQLFQQDITVKNVSFSYSMKAPTLNEISCTIPS
                     HSKTAIVGVSGSGKSTLAKLFVRFYEASEGSIHYGNINHLDIPFQILRDRVTYLPQES
                     FFFSGTIIENLIFGLDSPPSFEQIIEVCEIVQLNEFINQQPLRYETLLEEGGNNLSGG
                     QRQRLALARALLKNPDILILDEATSGLDTLLEHAIMNYLLNLKEKTIIFIAHHLPIAK
                     ACDQILVLHEGHLVEKGTHDELRFADGIYQKLWEI"
     gene            5644..5880
                     /locus_tag="IV334_002444"
     CDS             5644..5880
                     /locus_tag="IV334_002444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin"
                     /protein_id="HAP8944473.1"
                     /translation="MFKHAKCKGTKYCGNAQTVGGGNNAWGKLGQVVGGLTTGAVGGA
                     GLGTAICGPACGVVGGLYGAVAGGAAAGWDARKK"
     gene            5899..6114
                     /locus_tag="IV334_002445"
     CDS             5899..6114
                     /locus_tag="IV334_002445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin"
                     /protein_id="HAP8944474.1"
                     /translation="MHIKNTKTTFILSSEELKNIQGGSAVGVLGTTFSGATAGVKLCS
                     AGGPYAIAACGVGGALLGAGFSMWTGA"
BASE COUNT         2105 a         1204 c          996 g         2349 t
ORIGIN      
        1 ctgttttttg attttttctt actaaaaaac ctattatgaa aatcattatc gaaaaaacta
       61 gggtaattcc tccaaataaa acaacattcc cgcttagatt gactaacata tctatatcct
      121 tactggttag ttctgtagta tttaaagtag ttgaatcaat agaagataga aacataccac
      181 ccatcaatgc taaaagacta cataaaatcc ctgttattcc actaattaaa atcaaaataa
      241 actctgtttt tctcttattc atttaattct ctccagatta agtgttttag aattagaaat
      301 tttttgacca tagttaatag cttctaatga acgaataaca accactgacg tgttagaaaa
      361 ataatcccca atatgttgtt tcttgggcgt gaaagcagcc accaaatacc caatttgaaa
      421 tacaaaattt tttaatataa acctacatat acactcacga ataaagatag tttgccaact
      481 tagctctttt tcctcaaagc tcactactct tatcccaaaa atcattttcc caattgtttg
      541 tcctgaattc agcttagtca aaagtacaaa ataggttaaa taaatagcta atgacgctat
      601 accataaaaa gtaataatgg atttgtcaga ttccagtccc atgattttgt aaaagagacc
      661 tatagtagca tgggtgatga tgctaataca aattaaatca ataagaaaag caaaaaatcg
      721 taaccaaaat cccgcaaata cataattagg caaagtttca atgccactct tcttattcat
      781 tttatagccc tccatagtaa tacattggtt ttggagtatc agaaaatcca agtttttcaa
      841 aaactgaaat tacacgatct gatgtagttg cctttagccc ttgccattca gcaatcttat
      901 tacctaacca agaagaagca aagccagttg tgtcattttt atattctata accgtcgcat
      961 cagttaattt cttctctttt tttagggaat ctaatgcatc ttcagagtaa ccaatttgat
     1021 ccaccaaccc attttctact gcttggttac catcataaat tcttccatcc gcaagttctt
     1081 tcactgcatt agtatccata tttcttcctt ttgctacaac attgacaaaa cgatcataag
     1141 cactcatcac aaattcctgt ataactttat tctcttcctc tgaaggtttg tgttgaggtg
     1201 acatcatatc ttttaatgca ccacttttat acgtcgcatc agttacgcca agtttttcta
     1261 ataagccaga ataatttaaa ccagaaataa tgacaccaat tgatccagtt gttgtttctt
     1321 ccgtagcaaa tattttatct gaaccagcag aaatgtagta ccctccactt gccgctgtat
     1381 ttttaaatgc agtatagata ggaatatcta gctttttaat ttttgccatt tctttagcaa
     1441 tctcagcact ttcatatatt cctcccccag gtgaatttac ttctaataac actcccttaa
     1501 ccgctttgtc ttcttgaatt ttctttagct gtgttaaaaa attctgatgg ttatattgtt
     1561 ccctactaaa aagattgctt tcacctgtat ctgcaataac tccattgacg gatagtttga
     1621 caatcttttt atttgatgcg ccttcttcta acgtttcttc tgtgatttcg ttagatccgt
     1681 aaaaaatctt atttattcct gttagggttg tatttgatgc ttcctcttta tcttttcctg
     1741 gaattagcgc gacgataatg gatagtaaca ataatccgca cgctactccc acagcaatcc
     1801 atcttctttt attcatataa actcctccta tattcttagt aaaaataatt attttttatt
     1861 tagaaacaca tcgacaattt gttgccaata tgttttttct cctacaatta aagacaactc
     1921 tccagttaat ccataacgaa acggaaacga ttgagttgtt tttaacgttc ctttcactgc
     1981 ataaaaaaca cctttctcag tggcagtaga attttctgaa atttcatcta aagttccgtc
     2041 gatcgtaatc ggagccccac ctttttgatc caatttaaaa tgaaccttca tcccttcttt
     2101 aatataggta ctttcattcg caggtaaaag tgctgtaaat ttcatttttg atttttcgtg
     2161 gatcggataa atttctgcaa gcactgttcc tttggctagt tctgcttgtc cctttgtttc
     2221 ttcatttaaa tgaaccgttc cagatacagg agcttttagt attccttttg aaagttgatc
     2281 gtccaaactt ttgataactg actggttctt ctcttgtttt tcatttagct ctgtaactgt
     2341 ttgttttaca gttgttattg cttgttctag ctcttgcttt cctttttccg tttgactaat
     2401 tctgtcattc tcagaagtag ttggtttaac taatttagct ttttctgact gatactgttc
     2461 tacttctttt tttagttgct caatttgttc atttatgctt gctgaaattg tcagtttcac
     2521 ttgatttttt tgatcatcag aaacattatt tagttgttct tgccaattct catagtttgc
     2581 catcatttct tttgaaaaat cttttaatga ttgattatta ctccaagcta cctgtacttg
     2641 ttgccattct tgtaatttac tatctgaact ctcaatttgt ttttctagtt gtctttttgt
     2701 cttttcgtat acttctaatt gcttttgatc atttaattca ctttgcttca aagcgtagcg
     2761 gagtgattct ttttctgaca acatgctctt taattgatta ctgtagccaa aactgtcatc
     2821 cgttgaaaac aaattttttc ctttacttat actttcaata aatagctgag ccatctttat
     2881 ttgttgagta atacgttggt tttcttgttc tatctgagcc ttttcattct ctattaaaga
     2941 acaatccaat ctaaccaaca catcgtcctt atgaacaaat tgattttctt ttaggaagtt
     3001 ttccattatt ttagcttcta atggcacttg taacttctct attttttcgg ggttcaattg
     3061 agccgttgtc cgaataacaa cctctttttt agcaaaggtt aaaaacaatc caataataca
     3121 taaaaaaaga attacagggt ataacaccca acggtagaag ctatgatgtt gtttagtata
     3181 gatactagaa tgttcgaacc aattattatt ctgcataaat tgtttccctc tcctagattt
     3241 cccataattt ttgatagata ccatcggcaa aacgaagttc atcgtgcgtt cctttttcta
     3301 ccaaatgccc ttcatggaga actaaaattt gatcacatgc ttttgctatt ggtaaatgat
     3361 gcgcaataaa aataatcgtt ttttctttta aattgaggag ataattcata attgcatgtt
     3421 ctaacaaggt atctaatcca ctagttgctt catctaaaat taatatgtcc ggatttttta
     3481 ataaagcccg tgctaaggct aatcgttgtc gttgccctcc tgaaagatta tttccacctt
     3541 cttccaacaa tgtctcgtaa cgtaatggct gttgattaat aaattcattt agttggacta
     3601 tctcacaaac ttctattatt tgttcgaaac ttggtgggct atctaaaccg aatataagat
     3661 tctcaatgat tgttccacta aagaaaaatg actcttgagg caaatatgtc actcgatcgc
     3721 gtaatatctg aaaaggaatg tctaagtgat taatgtttcc ataatggatc gatccttctg
     3781 atgcttcata aaaacgaaca aataactttg caagagttga ttttccagaa ccactaactc
     3841 caactatagc cgttttactg tgcgaaggaa ttgtacatga aatttcgttc aacgttgggg
     3901 ccttcatgct atacgagaat gaaacgtttt ttacagtgat atcttgttga aataattgtt
     3961 cagaaagcat atattcaaaa gactggttcg atttttctga atcaatcgcg aaaatttcat
     4021 ttaaacgatt atttgcaact tgagccgttt gcattttcac ttgtaaatta ataatgtttt
     4081 gtaaaggatc tgtcaaaaat actagtaaag cattgtatgt gataagttga cctagactaa
     4141 ttctattatc cataacaaaa taagaaccta gccataaaat taatgcactg ctaattaatt
     4201 gaatcaaatg tttaattcct tgttgaatat tatcaagcgt aaccgtacga aatgattttt
     4261 tcattaattt aacaaattca tggtctatac ggttatatac tttctcttca ccgctatatg
     4321 ctttaattgt ttctattcct ttaagacttt ctatgatact agaatttaca gctgcaccgg
     4381 ctgccatttc ttccctgttc gatttttcat agcttttcac gaaagcgaat attgcaacta
     4441 cataaaaagg caaagagcat agagtgatta aaaacaacgt actattctga atagctaacg
     4501 taaccccaac aagcaacacc ataccaatat ctaaaaatat tgataacgta gcacttgcta
     4561 aagcatcaat aatcttactg gcatcaagaa aacgagaaat aatttcccct gattttctcg
     4621 tagcaaaaaa attcattggt aaagttaaca catgtttgaa atactgaagc ataattgcca
     4681 tactcattcg ctgtcctaaa ataataagaa gataactacg actatattca aacaataccc
     4741 gaaataaata gacagcgatc aagccaatgg aaattatatt taaagttgaa cgtgcttgat
     4801 tagggatgaa aaaatcaaga ataccttgaa aataatatga gcttcctatg ccaaaaaacg
     4861 taatgaaaat cgatgctaaa acgatgtgga atactaatcc tttttgtttc caaattatag
     4921 gcaaaaaaga acttaatcca gctacttttt ctttaatagg aatataagtt tctctaggca
     4981 taggtaataa aagaatacct gtccattctt tttgaaacgc ttcaatcgtt tttctatttt
     5041 ttccattagc gggatctgca atccatagta cgtttccctt cgttttataa attacaacgt
     5101 agtgcatata gctgttatca accaatatat gtgcaattaa tggtaagata agatctcttt
     5161 ctttccagat ctcactatct gcttgaatag ccatacaatc aaaagacaat ttttcaaatg
     5221 tctttttcaa tccaaatgct gaagtaccct ctaaatcagt cccagatagg tcccgtaatt
     5281 tatgaatcgg gatatcagtt ttaaaatatt ttaaaatcat cgaaaggcaa gcaacaccac
     5341 aatctttttc atcttgttgc tgcacaaaag gaaatgattt tatcacttat ctaacacaac
     5401 tccttctaaa aaatttttat agaaaaattc tcaatatagc tttaatacag tgaaaatatt
     5461 gtctcataat tccacaaaaa ctttgaaaac agttcttgaa cactaacttt ttcattcctg
     5521 aatcaaattt attttggaaa ttttactatt caagaataaa cttgataagt ttcaggaaga
     5581 tttttactgt taatcaaata cgtatgttac agtagcaccg taaaaaatat tatttaaaga
     5641 agcgtgttta aacatgcaaa atgtaaagga actaagtatt gtggaaatgc acaaactgtt
     5701 ggtggaggca ataatgcttg gggaaaattg gggcaagttg taggtggact taccacaggg
     5761 gcagtaggtg gtgcaggttt aggaacagca atttgcggac cagcttgcgg tgttgttgga
     5821 gggttatatg gagctgtcgc tggaggtgct gcagctggct gggatgctcg aaaaaaataa
     5881 aaaaggagaa taagtagtat gcatattaaa aacacaaaaa caacatttat attatcttca
     5941 gaagagttga aaaatattca aggtgggagt gccgtgggag ttttaggcac tactttttct
     6001 ggggcaacag caggagtaaa actttgttca gccggaggac cttatgcaat agctgcttgt
     6061 ggtgtaggtg gagctttatt aggtgctgga ttcagcatgt ggaccggtgc gtaaacaatt
     6121 aaaaaatatc aaacctcaag attggtttgt gtcgattacg aacataggta tgttctttat
     6181 cttttcaaaa gtttggaatg gacagaactt tttactaaag cttcttgtgc tagtttttat
     6241 aattatatta aatttttatc ttttattaaa gtcagataaa aattctttgg aaatgtaatg
     6301 gtagagatat ttttacaaaa ggacttgcag cgctgtagtt ggtgctgcag gaggacctat
     6361 ttcggccgtt acaggaggta ttggtaatgt acttgtcgga tgtgcattaa agcctgttcc
     6421 tgcaagttaa atttataaaa acaggaaagt aattatctca aatcctatca agtacaatca
     6481 taggattgca cttgacagga caaatttttt aactaaaaaa tgaaaaacac tttctaaaaa
     6541 ttagtagaat ttgccaaatc tttcgcatct tccaccatac atttaatttt aattcccctt
     6601 tatggtactt aataaaaaca agaaaagaac tattcatttg gccctttttt ggtt
//
LOCUS       DACDKM010000120         6535 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20834_2016
            SAMN12640370-rid9891663.denovo.120, whole genome shotgun sequence.
ACCESSION   DACDKM010000120 DACDKM010000000
VERSION     DACDKM010000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640370
            Sequence Read Archive: SRR10040891
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6535)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6535)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742835.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742835.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:04:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,012
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 29 of 75
            Pseudo Genes (incomplete)         :: 33 of 75
            Pseudo Genes (internal stop)      :: 19 of 75
            Pseudo Genes (multiple problems)  :: 5 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6535
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640370-rid9891663.denovo.120"
                     /isolate="E20834_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            51..2726
                     /locus_tag="IV334_002446"
     CDS             51..2726
                     /locus_tag="IV334_002446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF927 domain-containing protein"
                     /protein_id="HAP8944475.1"
                     /translation="MKMNHTSIQKHLQLLNTLGISYVNLRTFSTLNKKTVSHIFSTAD
                     SQAIINYLEKQKLSEGTVNITYNPIKQEVITQKKHSVRDNDIEKIRFVFIDIDPKRKE
                     GSATDSEKKKAENVMEQVERYLKEKRIESFVKVDSGNGYHIFLPINEQPNNHETILTI
                     QNFLQLLHRRFGVEDEVDIDTSVYNPSRLCKLVGTPAVKGKSTKERPHRLSSFLSIPD
                     NIDRSNCFKILEQIVEENSMMSTSRVKSHPSFREVLPFIQADYKQWLAHYPELTYTIK
                     EGDHKGVTLLIFDECPLRKHTNNSNGSCLSVTADNKIRFHCLHASDKNKDIQDFVKKY
                     PLPKPVNPSVESLESNQEYYFDQFKLNQEGVFLVKEGQEEKVACPMFIKQIRKNIEQK
                     VITLVLAYYVDHCWDEIEIEGGCLQTNNFKQLAKHGLIIKARREAEVVDFLQIQRENA
                     PIFFEHESLGWKKENEAMIFQLQDSFGREIPSVLTKNSCYQLTTNGTEAEFDALVHKY
                     VLGTNLELAWSIGIASIVLGYLNVTKANTFLSLLINLQGKSTTGKTTTLHFIASLFGN
                     PTTVLRNMNATNKAIIKLASNNQGGVPLILDELGAATQNNFSSLIYQLASGEERLRLD
                     SNCQLMPQETFSVLTLTSSEERLENYFNEKLTGLKVRYIEFSDIKFTKDANHAEKVKS
                     LSSNTFGWGIKLFLLKLFEQGITKIEATFVEAQKKLWAVLPEHSLKQRIGNNLALIYS
                     GALLIQDILGYPLRLEHIQTVLINSYEQLIVDTSLGSEQTVFDRVKTLLVMNSHKFLT
                     NSDNKHTPSTIWGKVSIQKNGDIKVNIFPIPFEDLLRREFQVKDLTYLFRQLLDSGNM
                     CAEKGRRTKRIHMNRQVLPTYEILLPSSLKSYFRL"
     gene            2908..3147
                     /locus_tag="IV334_002447"
     CDS             2908..3147
                     /locus_tag="IV334_002447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8944476.1"
                     /translation="MTKNKMTLKAEVLLYIQEHFSNQAFFTKPIYLDFEIRGVSAGSI
                     GGTLQALKNEGYLENHFVQRSFNRRVVKKWYLVHS"
     gene            3163..4536
                     /locus_tag="IV334_002448"
     CDS             3163..4536
                     /locus_tag="IV334_002448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAP8944477.1"
                     /translation="MRVACYDRTSVYEEAKISNDAQYDAVKEYVLEKYEQIPQRFSDK
                     GESGEKAERPAFRQLRAWVCASEEEGVIYVWRYDRLARQAKIALEFIELCQQHQVQVY
                     SISEPLPASFSHASTNKLFVQLLFTFAEQQRNVIIENVRAGLSYKKQQRQYLSSAVPY
                     GYRLVNGKIQQEVHEARIVRYIFQLYLTKKYGYKKLCQRLTQQKLFFRERPFQPYHIY
                     SILKNPLYYGEIKGGSLGKYLGTFEPILSKATFLQAQEIRQSRCTAKKDTYLYLLRQK
                     IRCPFCGRHLSSKYQWNTKKTKTLHYYHCTDRSCQGIFLRAERVESEVLEKIQAFFQY
                     EGVYQALIAQLTQQLAEKRKDEKRKVIQQKKQEAQWIKAFEEGNCSLEELKQQLQALQ
                     KPMSTSSFLSLQQCEAKVKEWLQLKDLSSRQLLCDYVCRIEVTKDKTVCGIYFRQLTQ
                     NILERNE"
     gene            4540..6174
                     /locus_tag="IV334_002449"
     CDS             4540..6174
                     /locus_tag="IV334_002449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAP8944478.1"
                     /translation="MEMIQTKRVALYIRVSTQEQALEGHSIQAQEQNGRQFAERMGYE
                     VVEVYADEGISGKSTKNRFAYQRMMNDARNHRFDLLVIWKLTRLGRNMLDVLQTVEEL
                     LRYDVGLHSISEQFDVTTSTGKLMLQLLGSFGEFERNQIAENVQMSMQSLVKDKKRYA
                     GGRRLGYVSGIDTNGQKQLVIEPEEAKIVRLIYAKYLGGAGYRAIANFLNRQGYQTVK
                     KNAFSTTAVKDILRNKIYAGYLEYARYINWETKRRKGKNPRPILVKGTHEPIISEEDY
                     QKVQERLALEGKQPKWNHTGENVLTGLLRCPECGAPMAASNVTNTLKDGTKKRIRYYS
                     CSVFRNKGASVCHANSIRAEQAETFVADRLKEIVQLPEVLPRLVAALNEEIVRQSQPL
                     EQELVVLLERKEELKTKIEKWEAALEDSPELFPMLKDRLDELTEKRRQLHIRENEILG
                     IFQQGEPIQVKDVQRILTNLDRFLAQSEKKQIKALYRTFIEKITFDPNHKEMLEITMK
                     FTHAVVQQLNEQYQIAVSKTKDTAIFVLKTPFTLTI"
     gene            6337..>6535
                     /locus_tag="IV334_002450"
     CDS             6337..>6535
                     /locus_tag="IV334_002450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002395661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8944479.1"
                     /translation="MKKMGKCILCEKYTELTKEHVPPKKSGNTGGKKTRTGNLDDFLK
                     SDFTKGDFPKGIKRKPQGNVYY"
BASE COUNT         2294 a         1062 c         1311 g         1868 t
ORIGIN      
        1 ggtaatgtag tcatacaaat aaaaattttt tagaaagtga gtggtaatta atgaaaatga
       61 atcacacttc aattcagaag catttacaac tgttaaatac actggggatt tcttatgtta
      121 atttgagaac tttttctaca ttaaacaaga aaaccgtttc tcatattttt tctactgcag
      181 attctcaagc aattattaac tatttagaaa aacagaaatt gtctgaaggt acagtaaaca
      241 taacttataa tcctattaaa caagaggtga ttacacaaaa aaagcattct gtgagagaca
      301 atgatattga aaaaattcgg tttgtattta ttgatattga ccctaaaagg aaagagggaa
      361 gtgcaacaga tagcgaaaag aaaaaggcag aaaatgttat ggaacaagta gaacgttatt
      421 tgaaagaaaa gagaatagaa tcatttgtaa aagttgatag tgggaatggc taccatattt
      481 ttcttcccat aaatgaacaa ccaaataatc acgagacgat tcttactatt cagaattttc
      541 ttcaattact tcaccgaaga tttggagtag aagatgaagt tgatattgat acttctgttt
      601 ataatccttc aagattatgc aaattagttg gtacaccagc ggtcaaagga aagtccacaa
      661 aagagcgtcc gcatcgttta tctagtttcc tttctattcc cgataacata gatagaagta
      721 attgttttaa aattcttgaa cagatagttg aagaaaattc catgatgtca acctctagag
      781 taaaaagcca tccatctttt agagaagtgt taccttttat tcaagcagac tataaacaat
      841 ggttagctca ctatcctgaa ttaacttata ccataaaaga gggcgatcat aaaggagtta
      901 ccttacttat atttgacgaa tgtccattaa gaaaacacac gaataattct aatggttctt
      961 gtttgtctgt tacggctgat aataagattc gatttcattg tctgcatgct tcggataaaa
     1021 ataaagatat tcaagacttt gtaaaaaaat atccgttgcc aaaacctgta aatccttctg
     1081 tagaatcatt agaatccaat caagaatatt attttgacca gtttaaactg aatcaagaag
     1141 gagtattttt ggtgaaagaa ggtcaagaag aaaaagttgc ttgtccgatg tttattaagc
     1201 aaatacgcaa aaatatcgaa caaaaagtca ttacacttgt tcttgcctat tatgtcgatc
     1261 attgttggga tgaaatagaa atagaaggtg gatgtttaca aactaataat tttaaacaat
     1321 tagcaaaaca tggcttaatc attaaagcta gaagagaagc tgaagtcgtt gactttttac
     1381 agattcaacg ggagaatgct ccaatctttt ttgaacatga atctcttggc tggaaaaaag
     1441 aaaatgaagc aatgattttt cagttacaag attcttttgg acgtgaaatt ccttctgtat
     1501 taactaaaaa ctcatgctac caattaacaa ctaatggtac agaagcggaa tttgacgcat
     1561 tggttcataa gtatgtttta ggaacgaact tagaacttgc ttggtcgatt ggtattgctt
     1621 ctattgtgtt aggctattta aacgttacga aggctaatac ttttttgagt ttactaatta
     1681 acctacaggg caagagcaca acgggtaaaa ccactacgtt acactttatt gcctctttat
     1741 ttggaaatcc aactacggtt cttagaaata tgaatgctac caataaagcc attataaagc
     1801 tagcatcaaa caatcaggga ggtgttccac taatactaga tgagttagga gcagcaactc
     1861 aaaataactt tagctcgttg atctatcaat tagcttcagg agaagaaagg ctaagattag
     1921 attctaattg tcagcttatg ccacaagaga ctttttctgt tttgaccctt acctcttctg
     1981 aagagcgttt ggaaaattat ttcaatgaga aattaactgg tttgaaagtt cgatatatcg
     2041 aattttctga tatcaagttt acaaaggatg cgaatcacgc agagaaagtt aaaagtcttt
     2101 cttctaatac ttttggttgg ggtataaagt tatttctttt gaaactcttt gaacaaggga
     2161 ttacaaaaat tgaagcaact tttgtcgaag ctcaaaaaaa actatgggct gttttacctg
     2221 aacattcttt gaagcaacgc attggtaata atctagcttt gatttatagt ggagccctcc
     2281 taattcaaga tatactgggc tatcctttac gattagagca tatacagacg gtgcttatta
     2341 attcgtacga acagttaatt gtagatacta gtttaggaag tgagcaaacg gtatttgacc
     2401 gagtgaaaac attgttggtg atgaatagcc ataaattttt aacaaacagt gacaacaaac
     2461 atacgcccag tacgatttgg ggaaaagtat caattcaaaa aaatggggat attaaggtaa
     2521 atatttttcc tatcccgttt gaggacttgt taaggcgaga atttcaagta aaggatctaa
     2581 cctatctttt taggcaattg cttgattcgg gtaatatgtg tgcagaaaaa gggagaagaa
     2641 cgaaacgtat tcacatgaat cgtcaagttc ttccaacata cgagattcta ttaccttctt
     2701 cactcaaatc gtattttcga ttgtaattta gaataaacca aagtatgaaa gtgaggcacc
     2761 atgttatgga aatatttctt attgatttag caaaacaact gattacttat ctgtatgata
     2821 gcgagcaaac ccaaacggaa gtaaatgaag cttgatctat gcttcattta cttcattcaa
     2881 tcaaacatga aagaaaggaa aattctaatg actaaaaata aaatgacact aaaagcagag
     2941 gttcttctgt atattcaaga acatttttca aatcaagcct tttttacaaa accaatttac
     3001 ttggatttcg aaataagagg ggtatctgct ggatctatcg ggggtactct tcaagcgttg
     3061 aagaacgaag gatatttaga aaatcacttt gtgcaacgtt cgttcaatag aagagtcgtg
     3121 aaaaaatggt atttagttca ttcatagaga ggggaatcaa gcatgcgtgt ggcttgttat
     3181 gatcgaacgt ctgtttatga agaagcgaaa attagtaatg acgctcaata tgatgcagtg
     3241 aaagagtatg ttttagagaa atatgaacaa attccccaac gattttcaga caaaggagaa
     3301 tcaggggaaa aagcggagcg tccagctttt cgacagctta gagcttgggt ttgtgcgtca
     3361 gaagaagagg gggtgatcta tgtttggcgt tatgatcgat tggcacgtca agccaaaata
     3421 gccttagaat ttattgagtt gtgccaacaa catcaggttc aagtgtattc gatttctgaa
     3481 cctcttcctg cttctttctc acacgcgtca acgaataaac tatttgtgca attattgttc
     3541 acttttgcag aacaacaacg aaacgtcatt attgagaatg ttcgtgccgg actttcctat
     3601 aaaaagcaac aacggcaata tttgtcttct gcggtaccat atgggtatcg tttagtgaat
     3661 gggaaaatcc aacaagaagt acacgaagca aggattgttc gctatatttt tcaactttat
     3721 ttgaccaaaa aatatggcta caaaaagctt tgtcaacgat tgacgcaaca aaagcttttc
     3781 ttccgcgaac gtccttttca gccctatcat atttatagta tcctaaaaaa tccattgtac
     3841 tatggcgaaa tcaaaggagg atctttgggg aagtatctag gaacctttga accgattctc
     3901 tcgaaagcaa cctttttaca agcccaagag attcgccaat caaggtgtac agctaaaaaa
     3961 gatacgtatc tgtacttatt acgtcaaaaa attcggtgtc ccttctgtgg tcgtcatctg
     4021 agttcgaagt atcaatggaa tacaaagaag acgaagacgc ttcattatta tcattgtacc
     4081 gaccgaagct gccaaggaat ttttcttcgg gcagaaaggg ttgagtcaga agtattggaa
     4141 aagatacagg ctttttttca atacgaaggc gtctatcaag ccttgattgc ccaattaacg
     4201 caacaattag ccgaaaagcg aaaagatgaa aaacgcaaag taatccaaca aaagaaacaa
     4261 gaagcacaat ggattaaggc atttgaagag ggcaattgtt cactggaaga actcaagcaa
     4321 caactgcaag ctcttcaaaa gccgatgtct acttcttcgt ttctttcact ccaacagtgt
     4381 gaagcaaaag tgaaagaatg gcttcaacta aaagacctct ctagtcgaca attattatgt
     4441 gactatgttt gtcggattga ggtaacaaag gataaaacag tttgtgggat ttacttccga
     4501 caactgacac aaaatatttt agaaaggaac gaatgatcta tggaaatgat acaaaccaaa
     4561 cgagtcgcat tgtatattcg cgtgagtaca caagaacaag ctttagaagg acatagtatt
     4621 caagcacaag aacaaaatgg acgacagttt gccgaacgga tgggctatga agtggtagaa
     4681 gtttatgccg atgaaggaat cagtgggaaa tccacaaaaa atcggttcgc ctatcaacga
     4741 atgatgaacg atgctcggaa ccatcgtttt gatctactag tgatttggaa attgacccgt
     4801 ctcggacgca atatgttaga tgtcttacaa acagtcgagg aattattgcg atatgacgta
     4861 ggattacatt cgatctcgga acaatttgac gtgaccactt cgacaggtaa attgatgtta
     4921 caactccttg ggagtttcgg tgaatttgaa cgcaaccaaa ttgcggagaa tgttcaaatg
     4981 tcaatgcaaa gcttagtgaa agataaaaag cgttatgcag gaggccgacg tttaggctat
     5041 gtcagtggta ttgacacgaa tggtcaaaaa caattggtca ttgaaccaga agaggcgaaa
     5101 attgttcgtc tgatctatgc aaaatattta ggcggagcag ggtatcgagc gattgccaat
     5161 tttttgaatc gtcaaggata tcaaacagtc aaaaagaatg ctttttctac cacggcagtc
     5221 aaagatatct tacgcaataa aatttatgca gggtatttag aatatgcgcg ctacatcaat
     5281 tgggaaacca aacgacggaa ggggaagaat ccgagaccga ttttagtgaa aggaacccat
     5341 gaaccgatta tttcggaaga agattatcaa aaagtacaag aacgccttgc tttagaagga
     5401 aaacaaccca aatggaacca tacaggtgaa aatgtattga caggcttgtt acgttgtcca
     5461 gaatgtggcg caccgatggc agcgagtaat gtcacaaata ccctaaagga tggcacaaag
     5521 aaacggattc gatattattc ttgttcagtc tttcggaata aaggagcaag tgtgtgtcat
     5581 gccaattcga ttcgtgccga acaagcggaa acatttgtcg ccgaccgatt aaaggagatc
     5641 gttcagctac cagaagttct tccccgattg gtggcagcgt taaatgaaga aatagtgcga
     5701 caaagccaac cattggaaca agaactcgtg gtgttactgg aacgaaagga agaattgaag
     5761 actaaaattg aaaaatggga agctgctttg gaagatagtc ctgaattgtt tccgatgtta
     5821 aaagaccgct tagacgaatt gactgaaaaa cgacgacagt tgcacattcg agaaaatgaa
     5881 atcttaggga tctttcaaca aggagagccg attcaagtca aagatgtcca acggatattg
     5941 actaatttgg atcgttttct tgcccagagt gagaagaagc aaatcaaagc cttgtatcgt
     6001 acctttatag agaaaataac ctttgatcct aaccataagg agatgttgga aattacgatg
     6061 aaattcacgc atgccgtggt acaacaattg aatgagcaat atcaaatagc cgtatccaaa
     6121 acaaaggata cggctatttt tgtgttaaaa acacccttta cgttaacgat ctagtctagg
     6181 ttatatcaaa caacgttaga ggggcgctaa cgcttctcta gcgatttgaa atcgttagag
     6241 aagtagttag ggaggtcctt atcataaata gggatattta ttaatgaata tttgaaatat
     6301 aattgtatat aagaattaaa aagagtgggg atagatatga aaaagatggg taagtgtatt
     6361 ttatgtgaaa aatacactga acttactaaa gaacatgtac cacctaaaaa atcagggaat
     6421 actggaggta aaaagaccag aactggtaat ttagatgatt tccttaagag cgattttaca
     6481 aaaggtgatt ttccgaaggg gattaaaagg aaacctcaag gaaatgttta ttata
//
LOCUS       DACDKM010000121         6490 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20834_2016
            SAMN12640370-rid9891663.denovo.121, whole genome shotgun sequence.
ACCESSION   DACDKM010000121 DACDKM010000000
VERSION     DACDKM010000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640370
            Sequence Read Archive: SRR10040891
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6490)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6490)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742835.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742835.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:04:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,012
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 29 of 75
            Pseudo Genes (incomplete)         :: 33 of 75
            Pseudo Genes (internal stop)      :: 19 of 75
            Pseudo Genes (multiple problems)  :: 5 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6490
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640370-rid9891663.denovo.121"
                     /isolate="E20834_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(182..1144)
                     /locus_tag="IV334_002451"
     CDS             complement(182..1144)
                     /locus_tag="IV334_002451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018375965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAP8944480.1"
                     /translation="MVAKLTDVAELAGVSPTTVSRVINNKGYLSEKTKQKVHEAMKTL
                     GYKPNNLARGLQGKSPQLIGLIFPNISNIFYSELIEYLEIELFKHGYKTIICNSQNDP
                     AKEREYIEMLEANQVDGIISSSHNLGIADYERVRAPIVAFDRNLAPNVSIVSSDNFEG
                     GKLAAKTLQKNGCQNTIMITGNDNTDSPTELRALGFSFQNPNGKIFKIPNNLSTIRRE
                     MEIKSTIINHKPDGIFVSDDLTAILTMKIAHQLKLNIPEDLKIIGYDGTSFIEKFFPQ
                     LTTIRQPIDEIASLIVDILLKKIKGEKTNKDYILPISILSGGSI"
     gene            complement(1146..2585)
                     /locus_tag="IV334_002452"
     CDS             complement(1146..2585)
                     /locus_tag="IV334_002452"
                     /EC_number="3.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose-6-phosphate hydrolase"
                     /protein_id="HAP8944481.1"
                     /translation="MNLPREIRYRAYSDWTKDEMDKIKDNVKRSPWRASYHIEPKTGL
                     LNDPNGFSFFNGKYTLFYQNWPFGAAHGLKEWVHNESDDLVHFHVTGAELLPDTKRDS
                     HGAYSGSAYEIEGNLFLLYTGNVRNENWERDTLQIGAWMDKDYNITKGENVLIHKPAD
                     VTDHFRDPQMFNYEGQLYAIVGAQNLEKSGFIKLYKADDNNVETWEEVGNLDFGGTGS
                     EYMIECPNLVFVDKKPVLIYCPQGLDKSELNYGNIYPNTFKICQSLDIEKVKLVGASE
                     IQNLDYGFEAYATQGFNTPDGRALIVSWIGLPDIDYPTDKYDYQGAMSLVKELSIKDG
                     KLYQYPVEAITSLRASSEQFTAKAETNNTYELELTFPPNQKSELLLFSDSKGNGLSLI
                     VDTKEGKIILDRSKAGIQYATDFGTIRECSIDTKETTANIFVDNSIIEIFINKGEKVF
                     TSRVFPENHQNGIQIKSGEPTGKYFELKY"
     gene            2770..4716
                     /locus_tag="IV334_002453"
     CDS             2770..4716
                     /locus_tag="IV334_002453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS beta-glucoside transporter subunit IIBCA"
                     /protein_id="HAP8944482.1"
                     /translation="MNYSKVASDVIQAVGKDNLIAAAHCATRLRLVLKDDTKVNQKAL
                     DENPDVKSTFKINGQYQVIIGAGDVNFVYDELIKKTGLSEISTDDLKQIASKDKKFNP
                     IMALIKLLSDIFVPIIPALVAGGLLMALQNFLTAAGLFGPKSIEEMYPAIEGISSMIQ
                     LMSAAPFMFLPILVGISAAKRFGANQFLGAAIGMIMTTPDLGGSTEYWNIFGYHVSQT
                     NYAYQVIPVLAAVWILSILEKFFHKKLPSSIDFTFTPLLSVMITGFLTFTVIGPVMLL
                     LSNGITDIIVWLYNTTGFIGMGIFGGTYSLIVMTGLHQSFPAIETQLLSAWTNGIGHG
                     DFIFVVASMANVAQGAATFAIWFLTKNSKTKSLASSAGVSALLGITEPALFGVNLKYR
                     FPFFCALIGSGIATAVAGLLKVVAVSLGSAGFLGFLSINATSIPFYVLCELISFVVAF
                     AITYFYGKTKAAGIFKAEEIAEQTPVNSSKVATTDPISTDVESTQTKVSSETIVSPIS
                     GKVISLSSVNDPVFSSEAMGKGIAIKPNGNTVHSPVDGTIQLTFETGHAYGIKSNNGA
                     EVLIHIGIDTVSMGGKGFTQKVTTNQKVRKGDILGAFDRSMIEKEGLDDTTMIIVTNT
                     SDYSEVNIVAENTVTEGTDLLEIK"
     gene            4980..5852
                     /locus_tag="IV334_002454"
     CDS             4980..5852
                     /locus_tag="IV334_002454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HAP8944483.1"
                     /translation="MLYGAIEAGGTKFVCAIGDEEMTIKERVSFPTTTPEETMPLVID
                     FFKQYQADLAGIGIGSFGPIDIHRDSATYGYITSTPKLAWQNFDFIGTMKKEFPIPIS
                     WTTDVNAAAYGEYVFGSGKGLSSVVYYTIGTGVGGGALQEGRFIEGFSHPEMGHMLVV
                     PHPKDDFAGSCPFHGNCLEGMAAGPAIEKRLGKKGQEVAEDDPYWEIEASYIAQCAYN
                     TTLMFSPDVIIFGGGVMKQRHMLQNVHDAFAKLVNGYVETPELSKYIVTPALEDNAGT
                     LGCLALAREAVLHS"
     gene            6148..>6490
                     /locus_tag="IV334_002455"
     CDS             6148..>6490
                     /locus_tag="IV334_002455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HAP8944484.1"
                     /translation="MTYTHLTPNELVMIEAYFHQETPVAIVAKQLKRGRQTIYNVYNF
                     LKCGGTALECFEQYKENKRRCGRTEIIFPAEEKEYIAKRSTEGWTPDVIIGRAERTFS
                     CSVSTLYRRFKT"
BASE COUNT         2074 a         1129 c         1155 g         2132 t
ORIGIN      
        1 acagttgtgt aaaaatatca ttttagagca tgaaaaaagg agtggatttc tccactccaa
       61 aaaagtctaa cttttggggg tcactacaag ttgacaactc ctttttgttt acaaatcaat
      121 tggatatcta caaattgtat tttctaacga attttataga cattaaaaaa ttaatttaac
      181 gttaaatact tcctccagaa agtattgaga ttggtaaaat ataatcctta tttgtctttt
      241 cgcctttaat ttttttcaga agaatatcaa caataagact tgcaatttca tcaattggtt
      301 gtcttattgt tgtcaattgt gggaaaaatt tctcaataaa agaggtacca tcgtatccaa
      361 taatttttaa atcttctggg atattaagtt taagttgatg agcaattttc atagtgagaa
      421 tggctgttaa atcatcagaa acaaaaatac catctggttt gtgatttata atagttgact
      481 ttatttccat ttcacgacgg atagtagaca gattattagg aattttgaaa atttttccat
      541 ttggattttg aaaagaaaaa cctagtgcac gtaattctgt gggagaatcc gtgttatcat
      601 ttcctgtaat cataatggta ttttgacaac catttttttg taaagtctta gctgcaagtt
      661 ttcccccttc aaagttatct gaagaaacaa tggaaacatt aggagctaaa tttctatcaa
      721 aagcaacaat aggtgctctc acacgctcat aatcagcaat tccaagatta tggcttgatg
      781 agattatgcc atcaacttgg ttggcttcaa gcatttcaat gtattctctc tctttagcag
      841 gatcattttg actattgcaa ataatcgttt tatatccatg tttaaataac tctatttcga
      901 gatactcaat gagttcagag taaaagatat tactaatatt aggaaaaata agaccaataa
      961 gctgaggtga ctttccttga agtccacgtg ctaaattatt tggtttataa ccaagagttt
     1021 tcatagcttc atgaactttt tgttttgttt tttctgataa atatccctta ttattaatga
     1081 cgcgtgaaac ggttgttggg ctaactccag ctaattcagc gacatcagtt aattttgcta
     1141 ccattttaat attttaattc aaagtatttt ccagtaggtt cgcctgattt gatttgaata
     1201 ccattttggt gattttcagg gaatacacga ctagtaaaca ctttttctcc tttattaata
     1261 aaaatctcaa tgattgagtt atctacaaaa atgttagcag ttgtttcttt agtatcaatt
     1321 gagcattcac gaatcgtacc aaagtcagtg gcatattgaa tgcctgcttt gctgcgatct
     1381 aaaataattt ttccttcttt ggtatctaca atcaagctaa gtccatttcc tttactatca
     1441 gagaaaagaa gtaactccga tttctgattt ggaggaaaag ttagttcaag ctcataagta
     1501 ttatttgttt ctgcttttgc tgtaaattgt tcagaactag cacgtaatga tgtaatagct
     1561 tctactggat attggtaaag tttaccatct tttattgaaa gttcttttac aagactcatt
     1621 gctccctgat aatcatactt atcagttgga taatcgatat caggtagtcc aatccaactg
     1681 acaatcagag cacgaccatc aggagtattg aatccttgag tcgcatacgc ttcaaatcca
     1741 taatcaagat tttgaatttc tgaagcgccc acaagtttta ctttttctat gtcaagcgat
     1801 tgacagatct taaaagtatt cggatagata ttcccataat ttagctcaga tttatccaac
     1861 ccttgaggac aataaatgag aacaggtttt ttgtctacaa atactagatt tggacactca
     1921 atcatgtatt ctgaaccagt gccgccaaaa tcaaggttgc caacttcctc ccatgtttcc
     1981 acgttattgt catccgcttt gtaaagtttg ataaagcctg atttttctaa attttgtgct
     2041 ccaacaatcg catacaattg tccttcataa ttaaacattt gtggatcacg aaaatgatca
     2101 gttacatcag caggtttatg aataagaaca ttctcacctt tcgtaatgtt gtagtcttta
     2161 tccatccatg ctccgatttg aagtgtatca cgttcccaat tttcatttcg aacgtttcct
     2221 gtatagagta agaatagatt cccctctatt tcatatgcag aaccggaata agcgccgtgg
     2281 ctatctcgtt ttgtgtctgg aagaagttct gctcctgtta catgaaaatg aactaaatca
     2341 tctgattcat tgtgtaccca ttctttcaac ccatgagcag ctccaaatgg ccaattttga
     2401 taaaaaagcg tatattttcc gttaaaaaaa gaaaaaccat taggatcgtt taacaatcct
     2461 gttttaggtt cgatatgata gctagcacgc caaggtgatc gttttacatt atccttaatt
     2521 ttatccatct catctttagt ccaatcagaa taagcacgat atcgtatttc tcgaggtaag
     2581 ttcatatttt actccttcaa tttatctttt tacaactagt atggtaaacg ttttccgttg
     2641 aaaaatcaat catttcattt aaaaaaataa actatatgac aaacgtttga catattttta
     2701 agatatgcta aaataaattt gcatttcagt gaaccgtttt cacgtaaaaa taaaaaagga
     2761 gtgattcgaa tgaattacag taaagtagct tccgacgtaa tacaagcggt cggaaaagat
     2821 aaccttattg cagctgcaca ctgcgctaca cgtcttcgtc tcgtactaaa agatgacacg
     2881 aaagtgaatc aaaaagcttt agacgaaaat ccagatgtga aaagcacatt taaaattaat
     2941 ggacaatatc aagtgattat cggtgcaggt gatgttaatt tcgtttatga cgaacttatc
     3001 aaaaaaactg gactttcgga aatttcaaca gatgatttaa aacaaattgc tagtaaagat
     3061 aaaaaattca accccattat ggcattaatt aaattattat cagatatttt tgttccaatt
     3121 atacctgccc ttgttgcagg tggtttatta atggcattac aaaacttttt aacagctgct
     3181 ggactttttg gaccaaaatc aattgaagaa atgtatcctg cgattgaagg tatttcctca
     3241 atgatccaac tgatgtccgc tgcaccattc atgttcttac caattttagt aggtatttca
     3301 gctgctaaac gttttggagc aaaccaattt cttggtgctg caattggtat gatcatgaca
     3361 acaccagatc ttggtggctc aactgaatat tggaatattt ttggttatca tgtttcacaa
     3421 acaaactatg cttatcaagt tattccagtt ttagcagctg tttggatatt atctatatta
     3481 gaaaaattct ttcataaaaa attgccatca tctattgact ttacattcac gccattatta
     3541 tcagtaatga ttactggatt tttgactttc acagttattg gccctgtaat gttattgttg
     3601 tctaatggaa ttacagacat tattgtttgg ctatataaca ctactggatt tattggaatg
     3661 ggtatttttg gaggaacata ttcacttata gtcatgactg gtttacatca atcatttcca
     3721 gctattgaaa ctcaattact ttcagcgtgg acaaatggta tcggacatgg agactttatc
     3781 tttgtagttg catctatggc aaacgttgct caaggtgctg caacatttgc catttggttc
     3841 ttaactaaaa attctaagac aaaaagtctt gcttcctctg cgggtgtctc agcacttctt
     3901 ggaattacag agcctgcctt gttcggagtt aacttaaaat accgctttcc attcttttgt
     3961 gctttaattg gttcaggaat tgctacagca gtagcaggat tacttaaagt agttgctgtt
     4021 tcacttggtt cagcaggttt cttgggattt ctttcaatta atgcaacatc tattccattt
     4081 tacgtattgt gtgaattgat tagctttgta gttgcttttg caattactta cttctacggt
     4141 aaaacaaaag ctgctggtat ctttaaagca gaagaaattg ctgaacaaac accagttaat
     4201 tcttcaaaag tagcaacaac agatccaata tctactgatg ttgaatcaac acaaacaaaa
     4261 gtttcatcag aaacaattgt tagtcccatt tctggtaaag tgattagtct ttcttcagta
     4321 aatgatccag tcttttcatc agaagctatg ggaaaaggga ttgccattaa accaaatgga
     4381 aacacggtcc attctccagt agatggtact attcaactca cgtttgaaac tggacatgct
     4441 tatggtatta aatcaaataa tggtgctgaa gttctaattc atattggtat tgatactgta
     4501 tcaatgggag gaaaaggatt tacacaaaaa gttactacta atcaaaaagt tagaaaaggc
     4561 gatattcttg gagcatttga ccgttcaatg attgagaaag aaggtcttga tgatacaaca
     4621 atgattattg tgaccaatac ttcggattat tcagaagtaa atatagtagc tgaaaataca
     4681 gtcactgagg gtactgattt acttgaaatc aaataaatat atttccctga gtaaagatgg
     4741 aattttcttt ccaaaatcaa ataatataga ttaatcaaat atttgagaat aacattttat
     4801 ttaatctttt catatatcaa ttgtatgaaa aattattaga attttaaaaa atgataaaaa
     4861 ttcaaaaaga aaaaatagta gttatttact tgtcatgtta ctaaataaaa aaataattta
     4921 aatatgatat atagtcattt atttagtgat ttcattctaa aataaaggga gaaatgtcta
     4981 tgctatacgg tgcaattgaa gcaggcggca caaaatttgt gtgtgccatt ggcgatgaag
     5041 aaatgacgat caaggaaaga gtgagctttc cgacaacgac acctgaggaa acaatgcctt
     5101 tagtcattga cttcttcaaa caataccaag cagatttagc agggattggg attggctcat
     5161 ttgggcctat cgatatccat cgcgattccg ccacttatgg gtacattacc tcgaccccga
     5221 aattggcatg gcaaaatttt gatttcatcg gtaccatgaa aaaagaattt ccgatcccga
     5281 tttcttggac aacagatgtc aatgcggctg cctatggtga gtatgtcttc ggcagtggca
     5341 aaggattgag cagtgtcgtt tactatacga tcggtacggg tgttggcggc ggtgccttac
     5401 aagaagggcg ctttatcgaa gggtttagtc atccagaaat ggggcatatg ctcgtcgtcc
     5461 ctcatccaaa agatgacttt gctggcagct gtccatttca tggcaactgt ttagaaggga
     5521 tggccgctgg acccgccatc gaaaaacgct tagggaaaaa aggacaagag gttgcagaag
     5581 atgatcctta ttgggaaatc gaagcctcct atattgctca atgtgcctac aatacaacct
     5641 tgatgttttc tccagatgtg atcatttttg gcggtggtgt gatgaaacaa cgccatatgc
     5701 tgcaaaacgt ccacgacgca tttgcaaaat tggtcaatgg gtatgtcgaa acacctgagt
     5761 taagcaaata tatcgtgacc ccagcattag aagacaacgc tggaacgctc ggttgtttgg
     5821 cattggctcg agaagcggtc ttacattcat aaacgaaaaa aggctcttcg acatttagtg
     5881 ttggtagaga aagatacaac tgaaaattta gtcataacca gttaggcaga gtgcttaact
     5941 ggtttttgtt ttacttggaa gatcaagcct tgaatgagat aaattcttgc tcgaaagtta
     6001 tggaaatttc gatagccgta ggccacttgt attgtgcaat agttttttac acgccgtttg
     6061 tataattaag cgagacaaat aaaaaagcca tttatgttac actcaaatcg taatacccgc
     6121 taaagcatac aaggagagtg aacataaatg acttataccc atcttacacc aaacgagctt
     6181 gtaatgatag aagcatattt tcatcaagaa actccggttg ctatcgttgc gaagcagctt
     6241 aaacgtggac gccaaacaat ttacaatgtc tataactttc tcaaatgtgg tggaacagca
     6301 cttgaatgct ttgaacaata caaagaaaat aagcgacgtt gtgggagaac cgaaatcatt
     6361 tttcctgctg aggaaaaaga atacattgca aaaagatcaa ctgaaggttg gaccccagac
     6421 gtcattattg gccgtgcaga gcgaaccttc tcttgttcag taagtaccct ctatcgccgg
     6481 tttaagacgg
//
LOCUS       DACDKM010000122         6402 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20834_2016
            SAMN12640370-rid9891663.denovo.122, whole genome shotgun sequence.
ACCESSION   DACDKM010000122 DACDKM010000000
VERSION     DACDKM010000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640370
            Sequence Read Archive: SRR10040891
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6402)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6402)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742835.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742835.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:04:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,012
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 29 of 75
            Pseudo Genes (incomplete)         :: 33 of 75
            Pseudo Genes (internal stop)      :: 19 of 75
            Pseudo Genes (multiple problems)  :: 5 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6402
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640370-rid9891663.denovo.122"
                     /isolate="E20834_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..1402
                     /locus_tag="IV334_002456"
     CDS             <1..1402
                     /locus_tag="IV334_002456"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type I restriction-modification system subunit
                     M"
                     /protein_id="HAP8944485.1"
                     /translation="LYRELLSDEESKEDLIATIVDILGYAIAPEYLFNVLADQAKQAT
                     FQLNDLNKAFVQLASTYNQFNGLFDDVDLQSKKLGTDEQQRNVTITEVIKKLNDVDVL
                     EHDGDVIGDAYEFLISQFASEAGKKAGEFYTPHMVSDMMAQIVTLDQKERRFFSVFDP
                     TMGSGSLMLNVRNYLTHPDNVKYHGQELNTTTYNLAKMNLILHGVDAEEMNLRNGDTL
                     NKDWPTDEPYTFDAVVMNPPYSANWSADTTFLDDSRFNRYGKLAPKSKADFAFLLHGF
                     YHLKETGTMAIVLPHGVLFRGAAEGVIRQKLLEDGSIYAVIGMPANLFFGTSIPTTVI
                     VLKKNRQTRDVLFIDASREFVKGKNQNKLSEENIQKILETYAERKDVEKYAHLATFDE
                     IKENDYNLNIPRYVDTFEEEEPIDMVHVGNDIKKIRQEQQVLEKKLLEAISSLQTTPE
                     NEAWLQGALEVFKHEQ"
     gene            1392..2609
                     /locus_tag="IV334_002457"
     CDS             1392..2609
                     /locus_tag="IV334_002457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HAP8944486.1"
                     /translation="MSNDTQPEIRFPGFTDDWEQRKLGDFVVDYVEKTSVQNQFPMLT
                     SSQQKGIVLQEDYFANRQVTTENNIGYFVLPRGYFTFRSRSDNDVFVFNRNDIIDRGI
                     ISYFYPVFTLKSADSDFFLRRINNGIQRQLSIQAEGTGQHVLSLKKFKNIVAMFPSEE
                     EQQKIGTFFKQLDDTIALHQRKLDLLKETKKGFLQKMFPENGAKVPEIRFPGFTEDWE
                     ERKLGELGKTQSGIGFPDAEQGGSEGTPFFKVSDMNNIGNEYEMRNANHYVSNEQIER
                     KKWKPIKDVPAIIFAKVGAAIMLNRKRLVTSPFLIDNNTMAYLFNNTWDIYFGKILFE
                     TINLPRYSQVGALPSYNSSDIENISVKVPVKDEQQKIGTFFKQLDDTIALHQRKLDLL
                     KETKKSFLQKMFV"
     gene            2622..5771
                     /locus_tag="IV334_002458"
     CDS             2622..5771
                     /locus_tag="IV334_002458"
                     /EC_number="3.1.21.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HsdR family type I site-specific
                     deoxyribonuclease"
                     /protein_id="HAP8944487.1"
                     /translation="MKISHREEAEVEEQLIRVLGEGHNQWTYRPDLKSEEDLWVNLRQ
                     KIISNNQAELNDFPLTDKEFETIKTELLLRTKTPFDAAKWLKGENGMARITIEREDSQ
                     LGSVSLILYSNQDIGGGISTYEVVHQIAKRGSNIEARDRRFDVTLLINGLPIVQIELK
                     QVTAKDGVYQAFNQIKKYAEEGMFRNNIFSTLQLFVVSNEQTTRYFANALPKDLHPKF
                     LFSWRTKDNEKVENLYEFCKQVLNIPDAHRLIADYTIVSEDQDNKTLMVLHPYQVHAI
                     QALFIAANKHQSGYVWHATGSGKTLTSFVSTKLLARKSGIDRTIMLVDRKDLDNQTTT
                     EFTKFASEFNTGISSGNAKANSLIVGTGSAKELSETLLADANANVVIITTRQKLDAAL
                     KYAKKQEEKKGTNRFQKLMGQHIVFVVDECHRALSAENMEEIKKMFPKSTWFGFTGTP
                     IFPENRKQAKGQLARTTHDQYGEVLHTYTIKNALEDGSVLGFQVEHENTVEPTSLENK
                     IYRKLKEVETYAEYSSEQINRMIDQMEPVKKESYLDPAVFEADEHIQKVIHKMFRPDN
                     AYTKFDFRNGRPTKSAILTTSSIDMAKKYYRAIKEMTKEPDWLTKEFPNQLIREGRTM
                     EDPDFPRIAITYSLEENSRDSSAQQEEMQKIIEEYNQYYDTSWSLADIERYNGDINNR
                     LARKRAEFKQFGKQIDLAIVVDRLLTGFDAPTIQTLFVDRNLEYAGLIQAFSRTNRTY
                     PDKTKGLIVTFRKPATMEKNVEDATKLYSKAKEESGLIYPSYQESKKRFKQAYGKLKE
                     VSSMESIDEHTPLEIRIEYVKAFQELNRAYESLVTYDDYNDDMVKSPTLNNQIQLLEE
                     QIGVYETVKGSLIEEEPEKEGEDFSTIEFYSENTTKLYDIDSTYIDQLLGTYAANSSD
                     AREEIEKALAKLNKTEGVKEVYRQILNAFDVGTLDSNEDIFVIKRRYFTQASDDLIHV
                     FSNEWFVSEKELHASNLQYLPGEDPIPNMKAIINSKDYEGYKAKHPEAKPFKYPQEMK
                     RAWRKMLDDELIPLENELR"
BASE COUNT         2229 a         1062 c         1317 g         1794 t
ORIGIN      
        1 gttgtatcgt gaattacttt cagatgaaga atcaaaagaa gatctgattg ccacaatcgt
       61 tgatattttg ggatatgcaa ttgcaccaga gtatcttttc aacgtcttag cggatcaagc
      121 aaaacaagca acgttccaat tgaacgatct aaacaaagcc tttgtgcaat tggcatctac
      181 ctacaatcaa tttaatgggt tatttgatga tgtggatctc caatcaaaaa aactaggaac
      241 agatgagcaa caacgaaatg tgacgattac cgaagtcatc aagaaattga atgatgtaga
      301 tgttttagaa cacgatgggg acgtgatcgg ggatgcgtat gagtttctga tcagtcaatt
      361 tgcttcagaa gcagggaaga aagccggaga attttacaca cctcatatgg tttctgatat
      421 gatggcgcaa atcgtaacgc tcgatcaaaa ggaacgacga ttctttagtg tttttgatcc
      481 aacgatgggt tcaggttcat tgatgttaaa cgtacgtaac tacttaacac acccagataa
      541 cgttaaatat catgggcaag aactaaatac aacgacatac aatctagcaa aaatgaactt
      601 gattttacat ggtgtcgacg ccgaagaaat gaatctccgc aatggggata cgttgaataa
      661 ggactggcca acagatgaac cttacacatt tgatgcagta gtaatgaacc caccttattc
      721 ggctaattgg tcagcagata cgactttctt agatgattct cggtttaatc gttatgggaa
      781 attagcgcca aaatccaaag cagattttgc gtttcttctt catgggttct atcatttgaa
      841 ggaaacaggt accatggcca tcgtcttacc acacggagtt ttattccgtg gcgctgcaga
      901 aggggtcatc cgtcaaaaat tactagaaga tggcagtatt tatgctgtga tcggtatgcc
      961 tgcgaacttg ttctttggga catcgattcc aacgacggtg attgtgttga agaagaatcg
     1021 tcaaactcga gatgtattat tcattgatgc tagtcgtgaa tttgttaaag ggaaaaatca
     1081 aaataaacta tcggaagaaa acattcaaaa aatcctcgaa acctacgctg aaagaaaaga
     1141 tgtcgagaaa tacgctcatt tggcaacctt tgatgaaatc aaagaaaatg actataactt
     1201 aaatatccca cgctatgtag atactttcga agaagaagaa ccaattgata tggttcatgt
     1261 cggaaatgac atcaagaaga tacgacaaga acagcaagtg ctagaaaaaa aacttttaga
     1321 agctatctct tccctgcaaa caacacctga aaatgaagcg tggttacaag gtgcgctgga
     1381 ggtgttcaaa catgagcaat gatacacaac cagaaatccg tttcccagga tttacagatg
     1441 attgggaaca gaggaagttg ggggacttcg ttgtagatta tgttgaaaag acaagtgtac
     1501 aaaatcaatt tcctatgcta acttcatctc aacaaaaagg gatagttttg caagaagact
     1561 attttgcaaa tcggcaagta actacagaaa ataatattgg ttactttgta ctacctagag
     1621 gatatttcac gttccgtagt cgaagtgata atgatgtgtt tgtgtttaac cgtaatgaca
     1681 ttattgatag agggattata agctattttt atcctgtatt tactttaaaa tctgctgatt
     1741 ccgacttctt cttaagacgt attaacaatg gaatccaacg acaattatct attcaagcag
     1801 agggaacagg tcaacatgtt ctgtctctaa aaaagtttaa aaatatagta gcaatgtttc
     1861 ctagtgagga agaacaacaa aaaatcggca ccttcttcaa acaacttgac gacacgatcg
     1921 ctcttcatca gcgtaagtta gatttgctga aggaaacgaa aaaaggattc ttacaaaaaa
     1981 tgttccctga aaacggagca aaagtgccag aaatccggtt tcctggtttt acggaagatt
     2041 gggaagagcg taagttggga gaattaggta aaactcaatc gggaattgga tttcctgatg
     2101 ccgagcaagg tggttctgaa ggtactccat tttttaaagt ttccgacatg aataacattg
     2161 gtaatgagta tgaaatgaga aatgcaaatc attatgtatc taatgagcaa attgagcgta
     2221 aaaaatggaa gccaataaaa gatgtgccag cgattatatt tgctaaagtt ggcgcagcaa
     2281 taatgcttaa tagaaaacgt ttggtcacaa gccctttctt aatagataac aataccatgg
     2341 cttatttatt taataatact tgggatatct attttggtaa aatattgttt gaaactataa
     2401 atttgccaag atactcccaa gtaggggcat tgccttctta taacagttct gatattgaga
     2461 atatcagtgt taaggttcct gtaaaggatg aacaacaaaa aatcggtact ttcttcaaac
     2521 agctagatga tacgatcgct cttcatcaac gtaagttaga tttactaaaa gaaacgaaaa
     2581 aaagcttctt acaaaagatg tttgtctagg agggaagaag aatgaaaatt agtcaccgtg
     2641 aggaagcaga agtcgaagaa cagttgattc gtgtacttgg agaagggcat aatcagtgga
     2701 cgtatcgtcc ggatctaaaa tcagaagaag acctttgggt caatttacgc caaaaaatta
     2761 tctcaaataa tcaagcagaa ctgaatgatt ttcctttgac cgataaagaa tttgaaacga
     2821 tcaaaacgga attactgctg cgaacgaaaa ccccatttga tgctgcgaaa tggttaaaag
     2881 gtgaaaatgg gatggctcgg atcacgattg aacgggaaga ttcgcaattg ggttctgtct
     2941 cacttatcct ttattccaat caagacattg gtggaggaat ctctacttat gaagtggtcc
     3001 atcaaattgc aaaacgtgga agtaacatag aagcacggga tcgacgtttt gacgtcacct
     3061 tattaatcaa tggattaccg attgtccaaa tcgagctgaa acaagtaact gcaaaagatg
     3121 gtgtttatca ggcatttaac caaatcaaaa aatatgcaga agaaggtatg tttcggaaca
     3181 atatcttttc gaccttacag ttgtttgtgg tttccaatga acaaaccact cggtattttg
     3241 caaatgcctt accgaaagac ttgcacccta agtttttatt tagttggcga acgaaagaca
     3301 atgaaaaagt agaaaactta tatgagtttt gtaaacaagt gttaaatatc ccggatgctc
     3361 atcgtttgat tgcggattat acaatcgtca gtgaagatca agacaataaa accctcatgg
     3421 tgttacatcc ttatcaagta catgcgattc aagccttgtt tattgccgca aataaacatc
     3481 aatcagggta tgtgtggcat gcgactggtt caggaaagac gttgactagt tttgtatcta
     3541 ctaagctttt agcacgaaaa tcggggatcg atcgaacgat catgttagtg gaccgcaaag
     3601 atttagataa tcaaaccacc acagagttta caaaatttgc ttcggaattt aatactggga
     3661 tttcttccgg gaacgccaaa gcgaatagct tgatcgttgg aacgggaagt gccaaagaat
     3721 taagtgaaac cctgttggca gatgccaatg caaatgttgt aatcattaca acccgtcaaa
     3781 aactagatgc ggcgttgaaa tatgcaaaga aacaagaaga gaaaaaagga accaatcgct
     3841 tccaaaaatt aatgggccaa catatcgtgt ttgtagtaga tgagtgtcat cgagcgttaa
     3901 gtgctgaaaa tatggaagaa atcaaaaaaa tgtttccgaa atccacttgg tttggcttta
     3961 caggtacacc gatttttcct gagaatcgga aacaagccaa agggcaattg gcacgtacaa
     4021 cccatgatca gtatggagag gtcttgcata cctataccat taagaatgcg ctagaagatg
     4081 gctctgtctt aggatttcaa gtagagcatg aaaacaccgt ggagccgact tcactagaga
     4141 ataaaatcta tcggaaatta aaagaagtag aaacatatgc agagtattca tcagaacaga
     4201 tcaatcgtat gattgatcaa atggaacctg tcaaaaaaga aagttatttg gacccagctg
     4261 tttttgaagc agatgaacat atccaaaaag tcattcataa aatgttccgt ccagataatg
     4321 cctatacaaa atttgatttt cgaaatggca ggccaacgaa atctgcaatc ttaaccacga
     4381 gttccattga catggccaaa aagtattatc gggccatcaa agagatgacg aaagagcctg
     4441 attggttaac aaaagagttt cccaatcagc tcattcgtga aggacgaacg atggaggatc
     4501 ctgattttcc acgaatcgct attacctatt cattagaaga aaatagccga gattcaagtg
     4561 cccaacaaga agaaatgcaa aaaattattg aggaatataa tcagtattat gatacttcct
     4621 ggtcattagc agacattgag cgttataacg gagacatcaa taatcgtttg gcacgaaaaa
     4681 gagcggaatt caaacaattt gggaaacaaa tcgatttagc gattgtagtg gatcgtctat
     4741 taacaggttt tgacgcccct acgattcaaa ccctatttgt ggatcgtaat ctagagtatg
     4801 caggattgat ccaagctttt tcacgtacga atcgtacgta tcctgataag acaaaaggat
     4861 tgattgtgac gtttcgtaag ccagcgacaa tggagaagaa tgtcgaggat gcgacaaaac
     4921 tgtattcaaa agcaaaagaa gaatcaggtt tgatttatcc aagttatcag gaatccaaaa
     4981 aaagattcaa acaagcatat ggtaagttaa aagaggtttc atccatggaa tccattgatg
     5041 aacatacgcc acttgaaatc cgtatcgagt acgtcaaagc atttcaagaa ttgaatcgtg
     5101 cttatgaatc gttagtgact tatgacgatt acaatgatga catggtgaaa tctccaactt
     5161 tgaacaatca aattcaactt ttggaagaac aaattggcgt gtacgagacc gtgaagggtt
     5221 cattaataga ggaagaacca gaaaaagaag gagaagattt ttcaacgatc gaattttata
     5281 gtgaaaatac cactaagttg tatgatattg attcaactta tattgatcaa ttactaggaa
     5341 cttatgctgc gaatagttca gatgcacgag aagaaattga aaaagctttg gctaaattga
     5401 ataaaacaga aggagtcaaa gaagtttacc gacaaatttt aaatgcattt gatgtgggga
     5461 ctttagatag taatgaagat atctttgtca ttaaacgtcg ctattttaca caagcgagtg
     5521 atgatttgat tcatgtcttt tccaatgagt ggtttgtttc tgagaaagaa ttacatgctt
     5581 ctaatctgca atatctacca ggtgaagatc caattcctaa tatgaaagca attatcaaca
     5641 gtaaggatta cgaaggatat aaagccaaac atccagaagc gaagccattt aaatacccac
     5701 aagaaatgaa gcgagcatgg cgtaaaatgt tagatgatga gttgattcca cttgagaatg
     5761 aattgaggta aacgagaaga gttaagaaaa ttgtacgatt caaagatggt agtcaagtgg
     5821 aagtgtagaa aatagggata gatgtctttt atttgttcac attttataac gaagtattta
     5881 agacagctat tgaataatgt ttcctccacc tttttcattc atttacccca ccccgttgtt
     5941 atatgaccgt gttttgagag gaaccaataa aaagcatagg ctaactaggg gttccttttt
     6001 cttttgtttt tttggaatcc gcttaagtga aatgtatctg aaatgaaacg gagataaagt
     6061 gattttttgc tataatgaca aggttttagc tgtttcagat accatgtaac ccaaaatagt
     6121 ttattgagaa aataggagag tatttgatac taactagtag gaaaaaatga gataaaaatt
     6181 aaatggaatc atttcaggta atctcaaata aaaaaatata cagtcctgtg gcactttacc
     6241 tatgggactg tatatttttt actctcagcg ggcttgtttt gactccgtgt ctgactccgt
     6301 cgtattgatt ttaaatgata ttcgatgacc atttaacagg tcatttttga aatttttgtc
     6361 tctatatgac tcagtttgat aattattgat agatcaattt gg
//
LOCUS       DACDKM010000123         6303 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20834_2016
            SAMN12640370-rid9891663.denovo.123, whole genome shotgun sequence.
ACCESSION   DACDKM010000123 DACDKM010000000
VERSION     DACDKM010000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640370
            Sequence Read Archive: SRR10040891
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6303)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6303)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742835.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742835.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:04:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,012
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 29 of 75
            Pseudo Genes (incomplete)         :: 33 of 75
            Pseudo Genes (internal stop)      :: 19 of 75
            Pseudo Genes (multiple problems)  :: 5 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6303
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640370-rid9891663.denovo.123"
                     /isolate="E20834_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            334..714
                     /locus_tag="IV334_002459"
     CDS             334..714
                     /locus_tag="IV334_002459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="HAP8944488.1"
                     /translation="MSEQHQNLASDHSKKYTYRSEPKQISFRVSESEFAKLKQSAEAL
                     QMSVPAFVKAKAQNARLVTPKVAPDIAQAIARDLAKAGGNINQIAKWCNTHQHDVAPD
                     DAQRLSENLKIMQKELQKIWQQLK"
     gene            696..1610
                     /locus_tag="IV334_002460"
     CDS             696..1610
                     /locus_tag="IV334_002460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HAP8944489.1"
                     /translation="MATVKVSRTTSCSRAINYAEPRATVKTGINCDIDYAKSEMKQIR
                     TLYGKDDRVQAHLLIQSFRPGEITAEKANQLGAEYAKKIAPNHQIAIYTHTDKDHIHN
                     HIVINSVNLETGKKFQAHGQAFLNKCYDLNDEICLEHDLTITERGKKTEKRTMSEIKL
                     KEKNEPVWKDEIRFAIDQTMKNKKTRTYTQFCDSLKTFGIHCLNRGKNFTYELVHKKK
                     KVRSNKLGKDYEKETILHELDRRERTYNQQRTNELRRARASIRETAEEVRREQRTFTP
                     EQSEIRPTRRNHEQHVERGTDHQQELSL"
     gene            1855..2544
                     /locus_tag="IV334_002461"
     CDS             1855..2544
                     /locus_tag="IV334_002461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8944490.1"
                     /translation="MAFYLPYLLISASILGIIWLSYKAYQTRYSLKGSKIRFKRFLLV
                     GVIFALSIVLSGLFGVLEGNKRVSRSILLGDITQKYEAACDKKKKEQALAQKMEEFTA
                     CYEDMNNIFVDQEKRLTDKNMKKLTRFYQNLPEKQQKEVQEKYEQVKKDVQYFKDTQT
                     EEACCNLFSDTIPFSTSEQERKERQQSVTYERYETLLQQATNIQNPTKKETALNYLES
                     VKEWLDQQQQN"
     gene            complement(2665..2751)
                     /locus_tag="IV334_002462"
     CDS             complement(2665..2751)
                     /locus_tag="IV334_002462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011302749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="HAP8944491.1"
                     /translation="MFERFICPLLVEVCVSLFSYWLNNRNKK"
     gene            3427..4377
                     /locus_tag="IV334_002463"
     CDS             3427..4377
                     /locus_tag="IV334_002463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RepB family plasmid replication initiator
                     protein"
                     /protein_id="HAP8944492.1"
                     /translation="MANEIVKYHHELNTIPLRKFTPVEMNLFFSIVSRMREKGDKTVR
                     LTFDQLKDLSNYKATANNRFINDLEMTYDKLMDLRFGKRSANGLDIDRFVMFSEFKIR
                     GTAKDPYVDIQVYEKALPLLNNLDEWVRYSLQQFNELQSSYAKTMFRLLKQYRTKGFA
                     YFSKEDFHELLDIPKSYWNNQANVDNRVIKPIRQELTAIFKGLSIKKKYGKGRGKPVV
                     GYQFTFKPEIKKADDFNKHAPTKEEQNKALALADKQKEYEEPATTINEKIKQKKRELE
                     LLQQIKQLEKEKEALEKQTESALSKKEKKSLLSDLDNLFQ"
     gene            4398..4931
                     /locus_tag="IV334_002464"
     CDS             4398..4931
                     /locus_tag="IV334_002464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="HAP8944493.1"
                     /translation="MQPQETFTIKELSELFKMSRQAISKHIQKLDSSMIAKNERGYKV
                     VLRSGVLQLARNLGNQMLLDRLHEYEQDNDKNVNIPSKNVVTNKLLDQLREENEFLRE
                     QLALREQNNSHIQKLLDQQQQLTLQANKQIEKLQEKLQLTYTEEAPENDSTTLSEATE
                     TIDKQIDTEKKWWQLWK"
     gene            5567..6100
                     /locus_tag="IV334_002465"
     CDS             5567..6100
                     /locus_tag="IV334_002465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8944494.1"
                     /translation="MKKFTLGVALLTIGAVVLPSTTIVYASENSQVVSLNENNATENV
                     VIEYTEDGMVVTKYEDGYSAEDFANSSDIMTRAAVARWGKKEYTNIAVTTGVAANAIN
                     AAFWAGGKASTSMFGIPGWAIGALLTGASWTKKGASPGNAVAKEWDKNGNGWIGFYYQ
                     RGYDAAGRVVATRYSTL"
BASE COUNT         2323 a         1102 c         1174 g         1704 t
ORIGIN      
        1 aaaaaaaaat aaatcgatac attttagaag aatagggttg tttgaatatt acaaagcaac
       61 aatttcacct tcggtgaaat aagcgaaact ttacaaaaag tggctagcaa atgctagcct
      121 ttttttgtat tgtggagcgt ggattttgag ggggattcaa gggggagctt gcgctccttt
      181 gacaagcggt tatttgcacc aaatacggag gtatttggct agcaaatata gagcttgcca
      241 aaccctttta gtgagcttta ctcattgtac ctttttaggt acaatggtat tgcgaacgat
      301 acgtccgtag gacgtactga aagggtggga cttatgagcg aacaacatca aaatttggct
      361 agcgatcatt ctaaaaaata tacctatcga tccgagccaa aacaaatcag ttttcgagtg
      421 agcgaatccg aatttgcaaa gctgaagcag tcagctgaag ctttgcaaat gagtgtgccg
      481 gctttcgtga aagccaaggc acaaaacgcg cgtctggtga caccgaaagt ggcgccagac
      541 attgcccagg caatcgcgcg cgatttagcc aaagctggag ggaatatcaa tcaaattgcc
      601 aagtggtgca atacacacca acacgacgtt gctcctgatg atgctcaacg tttatccgaa
      661 aacctaaaaa tcatgcaaaa ggagctacaa aagatatggc aacagttaaa gtaagtcgaa
      721 ccacttcatg tagccgagca atcaattacg cagaaccacg agcaaccgtc aaaacaggaa
      781 taaactgtga cattgattat gccaaaagtg aaatgaaaca aatacgcacg ctttatggaa
      841 aagacgatcg tgtacaagca cacttattga ttcagtcctt tcgaccagga gaaattaccg
      901 ctgaaaaagc caatcaacta ggggcagagt atgcgaaaaa aatagcccct aaccaccaaa
      961 tcgcgattta cacacataca gacaaagacc atattcataa tcacattgtc attaactccg
     1021 ttaatcttga aactgggaaa aaatttcaag cccatgggca agcattttta aacaaatgtt
     1081 atgacctaaa tgacgagatt tgtcttgaac atgacttaac aatcacggaa cgaggaaaga
     1141 aaaccgaaaa acgaaccatg tctgaaatta aattaaaaga aaaaaatgaa ccggtttgga
     1201 aagacgaaat tcggtttgcg attgatcaaa caatgaaaaa caaaaaaact cgaacgtata
     1261 cccaattttg tgattcactt aaaacattcg gcattcattg cttgaatcgc ggaaaaaatt
     1321 ttacttacga attggtccac aaaaagaaaa aagtacgatc caacaaacta ggaaaggatt
     1381 acgaaaagga gacgatttta catgagctgg acagacgaga acgaacctac aatcaacaac
     1441 gaaccaacga gctacgaaga gctcgagcta gcattcgcga aactgcagaa gaagtacgaa
     1501 gagaacaacg aacttttacg cctgaacaat cggaaattag accgactcga agaaaccatg
     1561 aacaacacgt tgaacgagga acagaccacc aacaagaatt atcgctctaa aacgttaaaa
     1621 gacatcgaaa acgaaataaa gagacaacgt gcctttcttg cgccttatac ccaagaaagg
     1681 atacaaacta cgatggattc acaagtcaaa aaactccacc aagcgctatt agaccagcga
     1741 aaccaagaac aaaaaaaggc tttaattcaa cgatcgatgg atattcttgc ccccttatgt
     1801 tgcttagtac taacattatt agccttttgg ttggtatggg gagaataaac agacatggca
     1861 ttctatttac cctatctact catttctgcc agtattttag gaattatttg gcttagctat
     1921 aaggcttatc aaacaagata ttcattaaaa ggctctaaaa ttcgatttaa gcgattttta
     1981 ttggtcgggg ttatatttgc cttatcaatc gttttaagtg gtttattcgg cgttttagag
     2041 ggtaataaac gtgtttcaag aagcatttta ttaggggata tcactcaaaa gtatgaagca
     2101 gcatgtgata aaaagaaaaa agagcaagca ttagcacaaa aaatggagga atttaccgct
     2161 tgttatgagg acatgaacaa tatttttgtg gatcaagaga aacgtttaac agataaaaac
     2221 atgaaaaaac tcactcgttt ttatcaaaac ttaccagaaa aacagcaaaa agaggtacaa
     2281 gaaaaatatg agcaagtaaa aaaagacgtg caatatttta aagatacgca gacagaagaa
     2341 gcttgttgta acttatttag cgatacaatc cctttttcaa ccagtgaaca agaacgaaaa
     2401 gaacggcaac aatcggtcac atacgaacgt tatgaaacac tgcttcagca agcaacaaac
     2461 atacaaaatc caacgaaaaa agaaacggca ttaaattatc tagaaagtgt caaggaatgg
     2521 ttggatcagc aacaacaaaa ttgaatttga ttagcaatca tgttaaactg aacctgcaaa
     2581 tgatcaaaca ttagatacac gaaagcccca gagaggtcgg attctctggg gctttttttc
     2641 aggttgatgt cgcctttatt gattttactt cttgtttcgg ttattcaacc aataactgaa
     2701 taaagagaca caaacttcta caagaagggg gcaaatgaac cgttcaaaca ttagatacac
     2761 ctccttccag tcgcaagact gtcggatagg cgacaagtta ttatacctta caaaataaaa
     2821 tctgtgtggt gtttttaagt atcccttatt ttcgggttgt tagggcttgc cctgaccgcc
     2881 tgtaaggcgc tttaagaaca agataaggtt ttttacccaa gactgtctta aaacgactta
     2941 gacgagctat cagaggcttc ccccacctcc gttactgact tgtttttagc aaaggagcct
     3001 ttaggcgact gcgcagtaca atgtgagagc atagcgatca cactttttta gcgaccgtag
     3061 ggagcaaggt tttgaattgg cagttttgaa aaatgcgcga acctgttggt agaaaaaaga
     3121 agtggttttt aggcgggaaa ttttgtcacc ctcttttata atctcttttt aaaccttttt
     3181 ttaaacctct ttaccgtcgg ctgtgtcctt actccttcaa tggatacagc gttttataaa
     3241 ctacattttg tctgctttta aactacattt tgtctgcttt taaactacat tttgtctgct
     3301 tttaaactac attttgtctg cttttaaact ctttttttgc actcacgtat gttataatat
     3361 gcattatcac agacaaaacg ttgtttataa cgtctgtatc cattgaagga gtaaggagga
     3421 atatctttgg caaatgaaat cgtgaaatac catcatgaat taaataccat tccgttgcgg
     3481 aaatttacac cagttgaaat gaatctattt ttctctattg tgtctagaat gcgcgaaaaa
     3541 ggcgataaaa cagtccgtct tacttttgac caactaaaag atttaagtaa ttataaagca
     3601 actgctaata atcgtttcat taatgattta gaaatgactt atgataaatt aatggattta
     3661 cgttttggta aaagaagtgc taatgggctc gatatagata gattcgttat gtttagtgag
     3721 tttaaaatac gtggaactgc taaagatcca tatgtagaca tacaagttta cgaaaaagct
     3781 ttacctttac taaacaattt agatgagtgg gttcgctatt cactccaaca atttaacgaa
     3841 ttacaaagta gctatgcaaa gactatgttt cgattactaa aacaatatcg aactaaaggt
     3901 tttgcttatt tttctaaaga ggattttcat gaactattag atattccaaa aagttactgg
     3961 aacaaccaag caaacgttga taatcgtgtt ataaagccta tacgacagga attaaccgca
     4021 atcttcaaag gattatcaat caaaaagaaa tacggaaaag ggcgaggaaa acctgtcgtt
     4081 ggctatcaat ttacgtttaa accagaaatc aaaaaagcag atgattttaa taaacatgca
     4141 ccaaccaaag aagaacaaaa taaagcattg gctttagctg ataaacaaaa agaatacgaa
     4201 gaacctgcta caacaattaa cgaaaaaatc aaacagaaga aacgagaact tgaactcctc
     4261 caacaaatca aacaactaga aaaagagaaa gaagctcttg aaaaacaaac agagtccgca
     4321 ctttcgaaaa aagagaaaaa aagtttatta tctgatttag ataatttatt ccagtagcaa
     4381 cctaggaggc aaccagtatg caaccacaag aaacctttac aataaaggaa ttgtcagaac
     4441 tttttaagat gagtaggcaa gcaatatcga aacatatcca aaaattagat tcttccatga
     4501 tagctaaaaa tgagcgtggt tataaggttg tattgcgttc tggagtattg caacttgctc
     4561 gcaacttagg caaccaaatg ttgctagata ggttgcacga gtacgaacaa gacaacgata
     4621 aaaatgttaa tataccaagc aaaaacgtgg ttacaaacaa gttgctagat cagttgcgtg
     4681 aagaaaatga atttttacga gaacaacttg ccctcagaga gcaaaataat tctcatatac
     4741 aaaaactact cgaccagcaa caacaactca ccctacaagc caacaaacaa atcgaaaaac
     4801 tgcaagaaaa actacagttg acttatacag aagaagctcc agagaatgac tcaacgactc
     4861 tttctgaagc aacagaaaca atagacaaac aaattgatac tgaaaaaaaa tggtggcaac
     4921 tttggaaata actggaggga acattcatga caagaaaaaa tcttctattc ggttctattt
     4981 tactaggaat tctaggattc agtccgatgt tattgtcagt gatcaataaa gggaataaac
     5041 atggtgaatt gaaattgaat ccaaattatt acaacagtcc taacgaagaa ttgtttgtaa
     5101 aagaaacaga taaataataa aaaagactgc tctttcgagc agccagttaa aaaataatga
     5161 gtgtttattc ttgaaaaagc aacgcgctta tgaagcgcta ccaaaaattt atcatatttt
     5221 attaaaaata acaacccagt aaaaaatgac tggtaaaaat tcacaaagaa acggaaagaa
     5281 gcaaccgcag aaaatcaagc ttaaaaagag cttttgacct agaccaatcg aaaatcacaa
     5341 agtgagcgcc tgtatctatt gctacaggcg ctacttggca tagtgtacat tatgtcaaca
     5401 agaatttaat ataacatatt ttcctaatgc attctaacta taccacatta tcaaaaatat
     5461 aaattatctt tactaaataa aatattgaca agcctatacc ttttgtttta tgattttcac
     5521 aaagaaaacg ttttctatat atttttctgt atcaggagga aattctatga aaaaatttac
     5581 tttaggggta gctttgttaa ctattggggc agttgtttta ccgtcaacta ctatagttta
     5641 tgcttcagaa aattcccaag tggtatcatt aaacgaaaac aatgctactg aaaatgtagt
     5701 tattgagtac actgaagatg gtatggtcgt aactaaatat gaggatggtt atagtgcgga
     5761 agattttgca aattcttcag atatcatgac aagagctgca gttgcaagat ggggcaaaaa
     5821 ggaatataca aatattgctg taaccactgg agtagctgct aatgctatta atgctgcctt
     5881 ttgggcagga gggaaagcta gcacttctat gtttggtatc ccaggttggg caattggtgc
     5941 tttgttaaca ggtgcaagtt ggactaaaaa aggtgcttct ccagggaatg cagttgcaaa
     6001 agaatgggac aaaaatggta atggctggat tggattttat taccaaaggg gatatgatgc
     6061 agctggaaga gttgtagcta ctagatattc aacgttataa gttaaggagt gatgtttttg
     6121 atatctaaat cattaaaatc attaaaaaca tttactaaaa agaatccgca aatagctact
     6181 attcgtattt ttatatttgc tatagctgtt gctataattg ttaatatgct aagcggaggg
     6241 caattataca aatctggttt atttttagtg ttaatcttag ttgtcttaga cataataaag
     6301 tct
//
LOCUS       DACDKM010000124         6173 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20834_2016
            SAMN12640370-rid9891663.denovo.124, whole genome shotgun sequence.
ACCESSION   DACDKM010000124 DACDKM010000000
VERSION     DACDKM010000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640370
            Sequence Read Archive: SRR10040891
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6173)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6173)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742835.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742835.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:04:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,012
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 29 of 75
            Pseudo Genes (incomplete)         :: 33 of 75
            Pseudo Genes (internal stop)      :: 19 of 75
            Pseudo Genes (multiple problems)  :: 5 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6173
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640370-rid9891663.denovo.124"
                     /isolate="E20834_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            725..811
                     /locus_tag="IV334_002466"
     CDS             725..811
                     /locus_tag="IV334_002466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011302749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="HAP8944495.1"
                     /translation="MFELFICPLLIEVCVSLFSYWLNNRNKK"
     gene            complement(932..1621)
                     /locus_tag="IV334_002467"
     CDS             complement(932..1621)
                     /locus_tag="IV334_002467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8944496.1"
                     /translation="MAFYLPYLLIFASILGIIWLSYKAYQTRYSLKGSKIRFKRFLLP
                     GVIFTLSIVLSGLFGVLEGNKRISRSILLGDITQKYEAACDKKKKEQVLTQKMEEFTT
                     CYEEMNDIFVNQEKRLTDKNMEKLTRLYQNLPEKQQKEVQDNYERTKKDVQYFKDTQT
                     EEACYNLFSDTIPFSTSEQERKERQQSVTYERYKALLQQATNIQNPTKKETALNYLKS
                     VKEWLDQQQQN"
     gene            complement(1628..1906)
                     /locus_tag="IV334_002468"
     CDS             complement(1628..1906)
                     /locus_tag="IV334_002468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8944497.1"
                     /translation="MKEEQTTNKNYRSKTLQDIENEIKKQRAFLAPYTKERIQTTIDS
                     QVKKFHHALLNQRNKEQKKVIMQRSMDILAPLCCLIMTLLAFWLVWGE"
     gene            complement(1866..2780)
                     /locus_tag="IV334_002469"
     CDS             complement(1866..2780)
                     /locus_tag="IV334_002469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HAP8944498.1"
                     /translation="MATVKVSRTTSCSRAINYAEPRATVKTGINCDIDYAKSEMKQIR
                     MLYGKDDRVQAHLLIQSFRPGEITAEKANQLGAEYAKKIAPDHQIAIYTHTDKDHIHN
                     HIVINSVNLETGNKFQAHGQAFLNKCYDLNDEICLEHDLTITERGKKPEKRTMSEIKL
                     KEKNEPVWKDEIRFAIDQTMKNKKTRTYNQFCDSLKTFGIHCLNRGKNFTYELIHKKK
                     KVRSNKLGKDYEKETILHELDRRERAYNQQRTNELRRARASIRETAKEVRREQRTLTT
                     EQPEIRPTRRNHDQHVERGADYQQELSF"
     gene            complement(2762..3142)
                     /locus_tag="IV334_002470"
     CDS             complement(2762..3142)
                     /locus_tag="IV334_002470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="HAP8944499.1"
                     /translation="MSEQNQYLASNHSKKYTYRSEPKQISFRVSESEFAKLKQSAEAL
                     QMSVPAFVKAKAQNARLVTPKVAPDIAQAIARDLAKAGGNINQIAKWCNTHQHDVDPG
                     DAQRLSENLKIMQKELQKIWQQLK"
     gene            complement(3592..3879)
                     /locus_tag="IV334_002471"
     CDS             complement(3592..3879)
                     /locus_tag="IV334_002471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HAP8944500.1"
                     /translation="MDFTKEEKLLNAISKVYNEATIDDYPDLKEKLFLYSKEISEGKS
                     VGEVSMKLSSFLGRYILKHKFGLPKSLIELQEIVSKESQVYRGWASIGIWS"
     gene            complement(3899..4123)
                     /locus_tag="IV334_002472"
     CDS             complement(3899..4123)
                     /locus_tag="IV334_002472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin hiracin-JM79"
                     /protein_id="HAP8944501.1"
                     /translation="MKKKVLKHCVILGILGTCLAGIGTGIKVDAATYYGNGLYCNKEK
                     CWVDWNQAKGEIGKIIVNGWVNHGPWAPRR"
     gene            complement(4763..5296)
                     /locus_tag="IV334_002473"
     CDS             complement(4763..5296)
                     /locus_tag="IV334_002473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="HAP8944502.1"
                     /translation="MQPQETFTIKELSDLFKMSRQAISKHIQKLDSSMIAKNERGYKV
                     VLRSGVLQLARNLDNQRLLDKLHKHEQNNDKNAYIPNKNVVANKLLDQLREENEFLRE
                     QLALKEQNNSHIQKLLDQQQQLTLQANQQIEKLQEQLQLTYTEESSENSSTTLSEATE
                     TMDEQTKNWKKWWQLWK"
     gene            complement(5317..6159)
                     /locus_tag="IV334_002474"
     CDS             complement(5317..6159)
                     /locus_tag="IV334_002474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RepB family plasmid replication initiator
                     protein"
                     /protein_id="HAP8944503.1"
                     /translation="MREKGDKTVRFTFDQLKDLSNYKATANVRFVEDLKATYEKILSL
                     RFGRRSESGLSYDMFVMFTEFKINGDVENPYVDIRIYEKALPLLNNLDEWVRYSLQQF
                     NELQSGYAKTMFRLLKQYRTKGFAYFSKEDFLELLDIPKSYKQPDIDKRVIKPIRQEL
                     TAIFKGLTIKKKYGKGRGKPVIGYQFTFKPEIKKADDFNKHAPTKEDQNKALALADKQ
                     KEYEEPATTINEKIKQKKRELELLQQIKQLEKEKKALEKQTESALSEKEKKSLLSDLD
                     NLFQ"
BASE COUNT         1633 a         1151 c         1042 g         2347 t
ORIGIN      
        1 aaaaaaacaa attcatttca accggtgtaa attttcttaa aggaattgta tttaattcat
       61 ggtggtattt tacaatctca ttagccatat aatcacctca atccaattat gaatgacatt
      121 ttttaaatgt caagacattc atatttgacg ttcaaacaga caaaatgtcc ttcataaaca
      181 gacaaaatgt ccttcataaa cagacaaaat gtccttcata aacagacaaa atgtccttca
      241 taacattctg tatccattgg tagagtaagg acacagctta cggtaaagag gtttaaaaag
      301 aggtttaaaa agagatataa aaagaggggg gctaattttc ccgcctaaaa accacttctt
      361 ttttctttaa acaggttcgc gcatttttca aaactgccaa tccaaaacct tgcttcctac
      421 ggtcgccaaa aaggtgtgat cgctacgctc tcacattgta ctgcgcagtc gcctaaaggc
      481 tcctttgcta aaaacatgtt ggtaaagagg cctttgagcg gttctgaatg cttatctaag
      541 ccgttttaag acagtcttgg ataaaaatcc ttatctagct ttaaagcgcc ttacaggcgg
      601 tcagggcaag ccctaacaac cctaaaataa gcgatactta aaaacaccac acaagtttaa
      661 ttttttaaga tataataact tgtcgcctat ccgacagtct tgcgactgga aggaggtgta
      721 tctaatgttt gaattgttca tttgccccct tcttatagaa gtttgtgtct ctttattcag
      781 ttattggttg aataaccgaa acaagaagta aaatcaataa aggcgacatc aacctgaaaa
      841 aaagccccag agaatccgac ctctctgggg ctttcgtgta tctaatgttt gatcatttgc
      901 atgtttagtt taacatgatt gccgatcaaa ttcaattttg ctgttgctga tctaaccatt
      961 ccttgacact ttttagataa tttaatgccg tttctttttt cgttggattt tgtatgttcg
     1021 ttgcttgctg aagcagtgct ttataacgtt cgtaggtaac tgattgttgc cgttcttttc
     1081 gttcctgttc actagttgaa aaagggattg tatcactgaa taaattataa caagcttctt
     1141 ctgtctgcgt atctttaaaa tattgcacgt cttttttggt tcgctcatag ttgtcttgaa
     1201 cctctttttg ctgtttttct ggcaagtttt gataaagacg agtgagtttt tccatgtttt
     1261 tatccgtcaa acgtttttct tgattcacaa aaatatcgtt catttcctca taacaagtgg
     1321 taaactcttc cattttttgt gttaacactt gctccttttt ctttttatca catgctgctt
     1381 catacttttg agtgatatcc cctaataaaa tacttcttga aatacgttta tttccctcta
     1441 aaacgccgaa taaaccactt aaaacgattg ataatgtaaa tataacccct ggcaataaaa
     1501 acctcttaaa tcgaatttta gagcctttta atgaatatct tgtttgataa gccttatagc
     1561 taagccaaat aattcctaaa atactggcaa aaatgagtag atagggtaaa tagaatgcca
     1621 tttctattta ttctccccat accagccaaa aggctaacag tgtcatgatt aagcaacata
     1681 agggggcaag aatatccatt gatcgctgca taattacttt tttctgttct ttatttcgct
     1741 ggtttaatag cgcatggtgg aattttttga cttgtgaatc aattgtagtt tgtattcttt
     1801 ctttggtata aggtgcaaga aaagcacgtt gtttctttat ttcattttct atgtcttgta
     1861 acgttttaga acgataattc ttgttggtag tctgctcctc tttcaacgtg ttggtcatgg
     1921 tttcttcgag tcggtctaat ttccggttgt tcagtcgtaa gagttcgttg ttctcttcgt
     1981 acttcttttg cagtttcgcg aatgctagct cgagctcttc gtagctcgtt ggttcgttgt
     2041 tgattgtagg ctcgttctcg tctgtccagc tcatgtaaaa tcgtctcctt ttcgtaatcc
     2101 tttcctagtt tgttggatcg tacttttttc tttttgtgga tcaattcgta agtaaaattt
     2161 ttcccacgat tcaagcaatg aatgccgaat gttttaagag aatcacaaaa ttggttatac
     2221 gttcgagttt ttttgttttt cattgtttga tcaatcgcaa accgaatttc gtctttccaa
     2281 acaggttcat ttttttcttt taatttgatt tcagacatgg ttcgtttttc gggtttcttc
     2341 cctcgttcag tgattgttaa gtcatgttca agacaaatct cgtcatttag gtcataacat
     2401 ttgtttaaaa atgcttgacc atgggcttga aatttgttcc cagtttccag attgacggag
     2461 ttaatgacaa tgtggttatg aatatggtct ttgtctgtat gtgtgtaaat cgcgatttgg
     2521 tggtcagggg ctattttttt cgcatactct gcccctagtt gattggcttt ttcagcggta
     2581 atttctcctg gtcgaaaaga ctggatcaat aagtgtgctt gtacacgatc gtcttttcca
     2641 tacagcatgc gaatttgttt catttcgctt ttggcataat caatgtcaca gtttatccct
     2701 gttttgacgg ttgctcgtgg ttcggcgtaa ttgatcgctc ggctacaaga agtggttcgg
     2761 cttactttaa ctgttgccat atcttttgta gctccttttg catgattttt aggttttcgg
     2821 ataagcgttg ggcgtcgcca ggatcaacgt cgtgttggtg tgtgttgcac cacttggcaa
     2881 tttgattgat attcccgcca gctttggcta aatcgcgcgc gattgcctga gcaatgtctg
     2941 gcgccacttt cggtgtcacc agacgcgcgt tttgtgcctt ggctttcacg aaagccggca
     3001 cactcatttg caaagcttca gctgactgct tcagctttgc aaattcggat tcgctcactc
     3061 gaaaactgat ttgttttggc tcggatcgat aggtatattt tttagaatga ttgctagcca
     3121 aatattgatt ttgttcgctc ataagtccca ccctttcagt acgtcctacg gacgtatcgt
     3181 tcgcaatacc attgtaccta aaaaggtaca ataagtaaag ctcactaaag ctcactaaag
     3241 ggcttggcaa gctctatatt tgctagccaa atacctacgt atttggtgca aataaccgct
     3301 tgtcaaaggg gcgcaagctc ccccttgaat ccccctcccc cccactctcc acaatacaaa
     3361 aaagactagc aaatgctagc cactttttgt aaagtttcgc ttgtttcacc ttcggtgaaa
     3421 ttgttgcaaa tcaataatta ggtagagcta catttttttc gttgttcaaa gattcttatg
     3481 ccatttacta ttaataaatc tataaatatg ataatttttc ggctctataa tttatgggac
     3541 tgatgaatca gaattgattc ttcagtccca ttttcatttt attccaaaga attaactcca
     3601 aataccaata gaagcccatc ctctatatac ttgagattcc ttactaacaa tttcttgtaa
     3661 ttctattaaa gatttaggta atccaaattt atgttttaaa atatatcttc caagaaaact
     3721 acttaatttc atactaactt caccaacact ttttccctca ctgatttctt tagaataaag
     3781 aaagagcttt tcttttaagt caggatagtc atctatagtt gcttcattgt atactttact
     3841 aattgcattt aaaagttttt cttctttagt aaaatccatt ataataacct ccctaccact
     3901 accttctagg tgcccatgga ccatgattaa cccaaccatt aacaataatt tttccaattt
     3961 ctcctttagc ttgattccaa tctacccaac atttttcttt gttacaataa agaccatttc
     4021 catagtaagt agctgcatca acttttattc ctgtaccgat gccagctaga caagttccta
     4081 atattcctag aataacacaa tgttttaata ctttcttttt catgattgtt tttccttttc
     4141 gtttttttaa aattgcttgt tatattaaat aatcttagta attttattat ataacatttt
     4201 attatataac aatgattaac gtcataaaaa gtagttttga attcataatc gtttaaataa
     4261 aattataaga tcagttattt aaacagctag tagacataat gtgtatacta ccaagtaacg
     4321 cctgtaccaa gggatccagg cgctgatttt gtgaattttt attggtctag gttaaaagct
     4381 ctttttaagg cttggttttc tgcggttgtt tctttctaat tctttgtgaa tattgatcgg
     4441 tcatttttta aatacttgtt atttttgttt gtgtttgtta tgctttttac atatctcata
     4501 ttcatttatt attcttttct aaggttaata ataggataat ctctttcaga aagactgctc
     4561 gtacgagcag tcttttctta tttatcaagt ttttctacaa atacttcctc attttgactg
     4621 ttgtagtaat taggattgag ttttagttcc ccttttttat ttcctttgtt ggccactgat
     4681 aataacattg ggctaaagct taatattcct agtaggatag aaccgaatag caagtgtttt
     4741 tttgtcataa gtatttcctt cttcatttcc atagctgcca ccattttttc caattctttg
     4801 tttgttcgtc cattgtttct gttgcttcag aaagagtcgt tgaactattc tctgaagact
     4861 cttccgtata agtcaactgc agttgctcct gaagtttttc gatttgttgg ttggcttgca
     4921 gggtgagttg ttgttgctgg tcgagtagtt tttgtatatg agaattattt tgctctttga
     4981 gggcaagttg ttctcgtaaa aattcatttt cttcacgcaa ctgatctagc aacttgtttg
     5041 caaccacgtt tttgtttggt atataagcat ttttatcgtt gttttgttca tgtttatgca
     5101 acttatctag caacctttga ttgtctaagt tgcgagcaag ttgcaatact ccagaacgca
     5161 atacaacctt ataaccacgc tcatttttag ctatcatgga agaatctaat ttttggatat
     5221 gtttcgatat tgcttgccta ctcatcttaa aaagatctga caattccttt attgtaaagg
     5281 tttcttgtgg ttgcatactg gttgcctcct aggttgctac tggaataaat tatctaaatc
     5341 agataataaa ctttttttct ctttttccga aagtgcggac tctgtttgtt tttcaagagc
     5401 ttttttctct ttttctagtt gtttgatttg ttggaggagt tcaagttctc gtttcttctg
     5461 tttgattttt tcgttaattg ttgtagcggg ttcttcgtat tctttttgtt tatcagctaa
     5521 agccaatgct ttattttggt cttctttggt tggtgcatgt ttgttaaagt cgtctgcttt
     5581 tttgatctca ggtttgaacg taaattgata gccaataaca ggttttcccc gtccttttcc
     5641 gtatttcttt ttgattgtta gtcctttgaa gattgcagtt agttcttgac gtataggttt
     5701 tataactcgt ttatctatat caggttgctt ataactttta ggaatatcta ataattcaag
     5761 aaagtcttct ttggagaaat atgcgaaacc tttggttctg tattgcttta acagacgaaa
     5821 catagtcttt gcatagccac tttgtagctc gttgaattgt tgtaaagaat agcgtaccca
     5881 ctcgtctaaa ttatttaaca aaggcagtgc tttttcgtaa attcgtatat ctacgtaagg
     5941 attttctacg tccccgttga ttttaaattc tgtaaacatt acaaacatat cgtaagaaag
     6001 cccagactca cttctacgac caaagcgtaa agataagatt ttttcataag ttgcttttaa
     6061 atcttctaca aatcgtacat ttgcagttgc tttgtagtta cttaaatctt ttagttggtc
     6121 aaacgtaaaa cgaactgtct tatccccttt ttcacgcatt ctagacacga tgg
//
LOCUS       DACDKM010000125         6097 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20834_2016
            SAMN12640370-rid9891663.denovo.125, whole genome shotgun sequence.
ACCESSION   DACDKM010000125 DACDKM010000000
VERSION     DACDKM010000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640370
            Sequence Read Archive: SRR10040891
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6097)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6097)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742835.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742835.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:04:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,012
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 29 of 75
            Pseudo Genes (incomplete)         :: 33 of 75
            Pseudo Genes (internal stop)      :: 19 of 75
            Pseudo Genes (multiple problems)  :: 5 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6097
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640370-rid9891663.denovo.125"
                     /isolate="E20834_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(309..794)
                     /locus_tag="IV334_002475"
     CDS             complement(309..794)
                     /locus_tag="IV334_002475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8944504.1"
                     /translation="MHKGIYAGLLGTVILFSACGYKTKNSVKESSAAISTTEVGSTKS
                     TSTTSITNTSTGNVDSQHSQSSIQSFSISSTSEMQESTVPSLPLENSDKALLFLIAHR
                     SELQSDDIVISFYQKIDRDYLFTASSKQIRSQGGSGSVGFYRVSPEGSITMTDANGTP
                     F"
     gene            complement(933..1148)
                     /locus_tag="IV334_002476"
     CDS             complement(933..1148)
                     /locus_tag="IV334_002476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF378 domain-containing protein"
                     /protein_id="HAP8944505.1"
                     /translation="MKTLDAIALTLLIVGGLNWLLVGLFELDLVAMIAGGSTTIFAKI
                     IYIVVGICAIYCLKFFPMITRKVDERY"
     gene            1372..2172
                     /locus_tag="IV334_002477"
     CDS             1372..2172
                     /locus_tag="IV334_002477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAP8944506.1"
                     /translation="MSDWLDIAGKVVIVTGGSSGIGRSIVENLLKQNAQVANFDVTEC
                     RIQHENLLSLKVDVSSKTDIKEGIYKVMKHFKTIDGLVNNAGINIPSLLIDRNHPKSK
                     YELSEQVFDKMIAVNQKSVYLMSQAVGRILVQKGSGVIVNLSSESGLEGSEGQSCYAA
                     TKAAMNSFTRSWAKELGKRNVRVVGVAPGILEETGLRTQEYEEALSYTRGISVEQLRN
                     GYSRTSTIPLGRSGKLQEVADLVCYYLSERSSYITGVTTNISGGKTRG"
     gene            2191..4059
                     /locus_tag="IV334_002478"
     CDS             2191..4059
                     /locus_tag="IV334_002478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HAP8944507.1"
                     /translation="MTLVNRWYQLLKLLVTHKKVSIQEVQKEIKTSNQTIKKDIGELN
                     EEIAGIAKIIQKNKHFELEIYDFDFFDEIMQGKLKTASDFNSASKRIAYLLNRLIETE
                     DFLRMDDLSEEVGVSRGTVVKDLKTLKEMINDFDVKITGTPNKGLRIIGDELELRLLL
                     FYFVSPYFSETKTEQFLEEKQAIKHFQKKTNASKQEITLLTKVIGISLNRISSNYLLK
                     KPINRYSGCFEYIQEFNELINQLEEIYELTLSQYEKDFISFPLNIINTSITMIRKANS
                     DLRLYFDPMMMRIRRLDLIDLDESFLYQEMKQHLSLLINRVIFRYRLTDLFYGEIEKK
                     YPFSYQIASECIQALEAILQHKISKVEISYLTLYLELAFRYQKEHFSSKKVAIVHTTG
                     KGTALMIQRQIKRILGRDIQITHVSEEQYEKVDLNKYFAVFTTIPLKNVNEETPVIHL
                     TNLFDDSWLRAQWQKAKKKRLAGFHSTVFFYQIWKEKKTYQEYLNKLVAPLIRGYYVD
                     EEFEKRILKREYQQSTFVNKEFAFPHTTNQLSQHIVLSVGLITDQGLAETGGNGVIFL
                     FAIPEEMTAEVENELFELYDILFELIKMGIDEFIELAPTQESFLKLIKSKGERLHE"
     gene            4052..4543
                     /locus_tag="IV334_002479"
     CDS             4052..4543
                     /locus_tag="IV334_002479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR family transcriptional regulator"
                     /protein_id="HAP8944508.1"
                     /translation="MNDMYLFIVFAASAYVLQAVISWKQLKHFNQTYSELRRKGKVAI
                     GKRPGKIRSGTIVMLAIDDHDQVLEVRKMQGVTSLAKFHSLPVYAGEDIHYIDRYHPS
                     VRAEDRLTQAALENAREVYLRIEAGNEKSEQPSLPFIAMLNNFRIWKSTIKEKRSDKR
                     WIS"
     gene            4531..5085
                     /gene="srlA"
                     /locus_tag="IV334_002480"
     CDS             4531..5085
                     /gene="srlA"
                     /locus_tag="IV334_002480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174078.1"
                     /note="catalyzes the phosphorylation of incoming sugar
                     substrates along with their translocation across the cell
                     membrane; the IIC domain forms the PTS system
                     translocation channel and contains the specific
                     substrate-binding site; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIC"
                     /protein_id="HAP8944509.1"
                     /translation="MDIITKFAEGFMRLFQTGAETFISWMTGIVPVVLMLMVAMNTLI
                     AFLGEDRVTKVAKLSAKNPLTRYLVLPFLSAFMLGNPMSFTMARFLPEYYKPSYYAAQ
                     AQFCHTSNGVFPHINPGELFVWMGIAQGIETLGLNSMELAIRYLLVGLLMNFIGGWIT
                     DFTTGFVCRQQGIVLSKKVELSVD"
     gene            5103..>6097
                     /locus_tag="IV334_002481"
     CDS             5103..>6097
                     /locus_tag="IV334_002481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIB"
                     /protein_id="HAP8944510.1"
                     /translation="MYGDIKIVRGNGGYGGPLIIRPTKDKHKFIYVTGGGERPMIVDK
                     IEELTGMESINGFKTSIPDSEIALAIVDCGGTLRCGIYPKKGIPTINIVPTGKSGPLA
                     QYITEDIYVSAVGLEQIQPLMETDDNKETVNTASPDKKYKYDTSKKITEQKAEQQNWI
                     AKIGMGAGKVVATFNQSAREAVDTMIRTVIPFMAFVSLLIGVIQGSGIGNVFANIMAP
                     LAGNAVGLTIIGFICSLPFLSPLLGPGAVISQVIGTLIGVEIGKGNIPPQLALPALFA
                     INTQNACDFIPVGLGLEEAKAETVEVGVPSVLYSRFLNGIPRVIIAWVASFGLYS"
BASE COUNT         2156 a          968 c         1219 g         1754 t
ORIGIN      
        1 tcattaaaat aataagaaga aaatatgtca aaaggtaaaa aagtaaattc ttctatccaa
       61 atatagaaaa attgaatcta gaaaaccgcc cgacaataaa tttcattaac tcagttgacc
      121 gaaaatgaag tagataccaa aaatttatca agagagcaag tagagaagtg agtatattca
      181 taagagaaat agctgtaacg aattattagg ttaatgcaaa tgcttattta tactccgtat
      241 aactttctca gaaaaaatga taccaataat gacttgctag tactgtaaat ccgtataata
      301 tcatctaatc aaaatggtgt tccatttgca tctgtcatcg taatcgatcc ttcgggtgac
      361 actcgataaa aaccaacgct tccagatcct ccttgggaac ggatttgttt gctggaagct
      421 gtgaacaaat aatctcgatc aattttttga taaaagctaa taacaatatc gtcactttgt
      481 aattcggaac ggtgggcaat aagaaataga agtgctttgt cagagttttc taatggaaga
      541 gaaggtacag tgctttcttg catttcagat gttgaagaaa tgctaaacga ctgaatactt
      601 gattggctat gttgcgaatc tacattacca gtagaagtat tagtaatgga agtagtagaa
      661 gtacttttag ttgaaccaac ctcagttgtt gaaatggctg ctgaagattc tttaacagaa
      721 ttctttgttt tatatccaca ggcagaaaaa agtattacag ttcccaacaa gcctgcgtaa
      781 atccctttat gcatgatagc tcctcctaaa atagattatc tttattcagt atatcactta
      841 aacaaagaaa agcagatggt ttaaaatgaa aaaaccttcc caataaaggg aaggttcatg
      901 aagataataa cttttttaat aattatagct ttctaatatc tttcatcaac ttttctagta
      961 atcattggga aaaatttcaa gcagtagatc gcacaaatac ctacgacgat ataaatgatc
     1021 ttcgcaaaaa tagttgttga accaccagca atcatagcta ctaaatcaag ttcgaacaag
     1081 cctaccaaca gccagttcaa ccctcccaca atcaataaag ttaatgcaat agcatctaaa
     1141 gttttcatta gaattctcct ttcttaaatg ataaaacaat tatatatcaa aaagagaaac
     1201 attgcttata taacgctcag gaattttagc aatctatgca aaaagatgaa aatttttttt
     1261 gctcttccat tagtacacat tttttagctg aagctgtaaa tttcatgtct taattttttt
     1321 tagtgagctt ttatactgga tttgtaagaa aaaagaagga gaggatcaca tatgtcggat
     1381 tggttggata ttgcaggaaa agtagtaatt gtaactggag gttcctcagg aattggaaga
     1441 agtatcgtcg aaaatttgtt gaagcagaat gcacaagtag cgaattttga tgtaactgaa
     1501 tgccgtatac agcatgaaaa cctactatct ttaaaagtcg atgtgtcttc taaaacggac
     1561 ataaaagaag ggatatataa agtaatgaaa cattttaaga cgatcgatgg tctagtcaat
     1621 aatgcaggaa taaatattcc ttctcttcta atagatagaa atcatccaaa aagcaagtat
     1681 gaattatcag aacaagtttt tgataagatg atcgctgtca atcaaaaaag tgtatatttg
     1741 atgagccaag cagttggaag aatcttggtg caaaagggat caggagtgat cgtgaatctt
     1801 tcttctgaat caggattgga aggatcagaa ggacagagct gttatgctgc tacaaaagca
     1861 gcgatgaaca gttttactcg ttcttgggca aaagagttag gaaagaggaa tgttcgtgtg
     1921 gtaggtgttg caccagggat actggaagaa accggtttga gaacacaaga atatgaagaa
     1981 gctctttctt atacgagagg tataagcgtc gaacagctga gaaacggtta cagtcgaaca
     2041 agtacgatcc ctctaggaag gagtggtaag ctgcaagaag tggcggatct agtctgctat
     2101 tatctttcag aaagatcaag ttatatcact ggagtcacga ctaatatttc aggaggtaaa
     2161 acaagaggat aaaggatagg ggggacgcac gtgacgctag taaacagatg gtaccaatta
     2221 ttgaaattac tagtcactca caagaaagtt tcaatccagg aagtgcaaaa agaaatcaaa
     2281 accagtaatc aaaccataaa aaaagatatt ggggagttga atgaagaaat tgccggtatt
     2341 gcaaaaatca tccaaaaaaa caaacatttt gaacttgaaa tttatgattt tgattttttt
     2401 gatgagatca tgcaaggaaa attgaaaaca gccagtgatt tcaattcggc atccaaaaga
     2461 atcgcttatt tactaaatag gctgatcgaa acagaggatt tcttacgtat ggatgatttg
     2521 tctgaagaag tcggtgtcag cagaggaaca gtggtcaagg atctaaagac attaaaagag
     2581 atgatcaatg attttgatgt aaagattaca ggcacaccaa ataaaggact aagaataata
     2641 ggagatgagc tagaattacg actactttta ttttattttg tttctcccta cttttcagag
     2701 acgaaaacag aacagtttct agaagaaaaa caagcaatca aacattttca gaagaagacg
     2761 aatgctagta aacaagaaat caccctttta acgaaagtta tcggtatttc tttgaataga
     2821 atctctagta actatctgtt gaagaagccc atcaatcgtt acagtgggtg ttttgaatat
     2881 atccaagaat tcaatgaact gatcaatcag ttggaagaaa tctatgaact aactttgagc
     2941 caatatgaaa aagattttat cagttttcct ttgaacatca tcaacacaag tataacgatg
     3001 ataaggaaag ctaactctga tttgcgctta tatttcgacc ccatgatgat gcgtattcga
     3061 cgattagatc tcattgatct ggatgagtcc ttcttatatc aagagatgaa acagcacctc
     3121 tctttattga taaatcgggt aatttttcgt tatcgattga ctgatttatt ttatggtgag
     3181 atagaaaaaa agtatccttt ttcttaccaa atcgctagcg aatgcataca agcattagaa
     3241 gctatcttgc aacataagat ctctaaagta gagatcagct acctaacgtt gtatttggag
     3301 ttagcatttc gatatcaaaa agagcatttt tcctctaaaa aagtagccat tgttcatacg
     3361 acaggaaaag gaacagcatt aatgatccaa agacaaatca aaaggatatt ggggagggat
     3421 atacaaataa cacatgtttc agaagaacaa tatgaaaaag tagatctaaa caaatatttc
     3481 gctgttttta caacgattcc gttaaaaaat gtaaatgaag agacaccggt gatccatttg
     3541 actaatcttt ttgatgactc ttggttaagg gcacaatggc aaaaagcaaa aaaaaagcga
     3601 ttagccgggt ttcattctac tgtttttttc tatcaaatat ggaaagaaaa aaagacgtat
     3661 caagaatatt taaacaaatt agtcgctcct ttgatcagag gctattatgt ggatgaagaa
     3721 tttgaaaaaa ggatcttgaa aagagaatat cagcaaagca catttgttaa caaagaattt
     3781 gcttttcctc acacaaccaa ccagctatct caacacattg ttttatcagt agggctaata
     3841 actgatcaag gtttagcaga aacaggagga aatggagtta tttttttatt tgccatacct
     3901 gaagaaatga cggctgaagt tgagaatgaa ttatttgagc tatacgatat cctatttgaa
     3961 ctcataaaaa tgggaataga tgagtttatt gaattagcac cgacacaaga atcattttta
     4021 aaactaatca aaagtaaagg agagagactt catgaatgat atgtatctat tcatagtttt
     4081 cgcagcttca gcttatgtgc tgcaagcagt tatcagttgg aagcaactaa agcatttcaa
     4141 tcaaacatat agcgaactgc gaagaaaagg aaaagtagcg attggtaaac gacctgggaa
     4201 aatccgttca ggcacgattg ttatgctagc tatagacgat catgatcaag ttttagaagt
     4261 cagaaaaatg caaggggtta catctttggc aaaatttcat tcattacctg tctatgcggg
     4321 tgaagatatc cattacatcg atcgttacca tccctcagtt cgagcggaag atcgtttgac
     4381 acaagcagct ttagaaaatg ccagagaagt atacttacga atagaggcag gaaatgaaaa
     4441 aagtgaacaa ccttcattgc cttttattgc tatgctcaat aattttcgga tatggaaaag
     4501 tacgatcaaa gaaaaaagga gtgacaaacg atggatatca taacaaaatt tgctgaggga
     4561 tttatgaggc tgttccaaac aggtgcagaa acattcataa gctggatgac aggaatcgta
     4621 ccagttgtct tgatgctgat ggttgcaatg aatacgctga ttgctttttt aggtgaagac
     4681 cgagtaacga aagtagccaa attatcagcg aaaaatccgc ttactcgtta ccttgtgtta
     4741 ccgttcttat ctgcttttat gttgggaaat cctatgtcat ttacaatggc tcgtttcctt
     4801 ccagaatact acaaaccaag ctattatgct gctcaagctc aattttgtca tacgagcaat
     4861 ggtgtattcc cccatattaa tcctggagaa ttgtttgtat ggatggggat tgctcaaggg
     4921 atcgaaacct tagggctaaa ttcgatggag ctggctatcc gatacttgct tgtgggactg
     4981 ctgatgaact ttattggtgg ttggataact gattttacta ctggatttgt atgcagacag
     5041 caaggaatcg ttcttagcaa aaaagtagag ctttcagttg attagagaaa ggagagtttt
     5101 aaatgtatgg tgatattaag atcgtaagag gaaacggcgg gtatggaggt cctttgatta
     5161 ttaggccgac aaaagataaa cacaagttca tctatgtcac aggtggaggt gagaggccaa
     5221 tgatcgtaga taaaatagaa gaactgacag gtatggagtc tatcaatggg ttcaaaacat
     5281 caatacctga tagtgaaatc gcgcttgcaa tcgttgactg tggagggact cttagatgtg
     5341 gaatttatcc taaaaaaggg ataccaacca tcaatatcgt tcccacagga aaaagtggcc
     5401 cattagcaca atatatcact gaagatattt atgtttcagc agtagggtta gaacaaatac
     5461 agccactaat ggaaactgat gataacaaag aaacagtaaa tactgcatct ccagataaaa
     5521 agtacaaata tgatacttct aaaaagataa ccgagcaaaa agcagagcag caaaactgga
     5581 tagcaaaaat tggaatgggc gcgggaaaag tagttgctac attcaatcaa tcagcaagag
     5641 aagcagtaga tacgatgatc cggacagtga tcccttttat ggctttcgtt tcccttttga
     5701 ttggtgtgat acaaggttct gggatcggta atgtatttgc taacattatg gcacctttag
     5761 caggaaatgc agttggatta acaatcattg gctttatttg ttcactgcca tttttatctc
     5821 ctttgcttgg tccaggggca gttattagcc aagtgattgg aacattgatc ggtgtagaga
     5881 ttgggaaagg aaatattcct ccccaattag cattaccggc actatttgct atcaatactc
     5941 aaaatgcatg tgattttatt ccagtcggtc taggtttgga agaagcaaaa gctgagacag
     6001 tagaagtagg tgtaccatca gttctttatt caagatttct taacggcatc ccacgagtta
     6061 tcattgcttg ggtagcaagt tttggattat atagctg
//
LOCUS       DACDKM010000126         6002 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20834_2016
            SAMN12640370-rid9891663.denovo.126, whole genome shotgun sequence.
ACCESSION   DACDKM010000126 DACDKM010000000
VERSION     DACDKM010000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640370
            Sequence Read Archive: SRR10040891
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6002)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6002)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742835.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742835.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:04:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,012
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 29 of 75
            Pseudo Genes (incomplete)         :: 33 of 75
            Pseudo Genes (internal stop)      :: 19 of 75
            Pseudo Genes (multiple problems)  :: 5 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6002
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640370-rid9891663.denovo.126"
                     /isolate="E20834_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..395
                     /locus_tag="IV334_002482"
     CDS             <1..395
                     /locus_tag="IV334_002482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8944511.1"
                     /translation="ISLKDESNTTVSDANVLANIILDFIFEHHIPFKEGYEILPANQE
                     YYFYKCITKRVCCICGKTGADIDHFDKALGRRKRKEVDHSEYTFAALCRIHHTEKHKI
                     GVINFKNKYQIKGIKLSHETIKKLRIGG"
     gene            398..1228
                     /locus_tag="IV334_002483"
     CDS             398..1228
                     /locus_tag="IV334_002483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HAP8944512.1"
                     /translation="MAEISWIKLKTTMFDDEKIRLIQAVPESDAIIVIWIRLLVLAGK
                     TNDDGLIYIQRNMPYTEEMLATLFGKNVNTVRLALTTLANFNMIDLSSDGLIAISNWE
                     KHQNIEGMDKVRLKNAERNRKYRERKRQERLKLENDVSVTSRDGTDKDIEEDKDIDKE
                     EKKGKYSDEHLRLAKKMQRNLTEDFPKEMNKVDIEKWADTIRLMEERDKASIEAIEYV
                     INWLPTNEFWFGNIRSAKKLREKFEKLKFEIKADKKNHKKQSQKLQYSNPSEYDDLPI
                     "
     gene            1244..2095
                     /locus_tag="IV334_002484"
     CDS             1244..2095
                     /locus_tag="IV334_002484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HAP8944513.1"
                     /translation="MESLANAMEKLIRRVLVQSGKCPECSEPLYSWRAKNKDGSERCK
                     PTCMSCGYKALRVKEDIQTERIYNDSLKARALSFFQNGSVLTDKTLFKCKMENYHVVD
                     QETKIALERAKSYVNDVLLNHPAHFILSGKSGSGKSHLSMATAWEILERSNYDKKILF
                     ISYQELLEQIKFSYNNAELRKEIEGSLIADIKTTDLVVFDDIGAELGSGVSNSRQFTN
                     NTLNTLLEARQNKATIITTNLSGPELREAYGERIVSRIFKNSEGYALKFQQTADKRIK
                     PVKGSIA"
     gene            2092..2451
                     /locus_tag="IV334_002485"
     CDS             2092..2451
                     /locus_tag="IV334_002485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1064 domain-containing protein"
                     /protein_id="HAP8944514.1"
                     /translation="MNKYRNKKTVHRGIKFDSIAEAEYYDLALWQAEANGWKVKLQER
                     FELMPKFELDGKKYRKIEYIPDFTFYKNGKLVKVVDVKGMQTKDFKIKAKLFCSQYRV
                     PLILAKKYRNTFKEERF"
     gene            2466..2627
                     /locus_tag="IV334_002486"
                     /pseudo
     CDS             2466..2627
                     /locus_tag="IV334_002486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752308.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
     gene            2624..2929
                     /locus_tag="IV334_002487"
     CDS             2624..2929
                     /locus_tag="IV334_002487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8944515.1"
                     /translation="MSVDKKAAMKRIIELTHSENWQEDKEIVAEVQKLGKSMWAEKPK
                     RKTPRKIAIWHGDRILVTGTAEQLSEITGLSKNIIWDRARSLWIDSKGRQFRYVEEK"
     gene            2929..3240
                     /locus_tag="IV334_002488"
     CDS             2929..3240
                     /locus_tag="IV334_002488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8944516.1"
                     /translation="MNELITKVEQWAKDKGLDQADPKAQFLKVAEEFGEIASAMARSN
                     DELFKDSVGDVIVTLIILSMQKGANIQECLEMAYNEIKGRTGKMVDGVFVKSSDLEDS
                     E"
     gene            3407..3823
                     /locus_tag="IV334_002489"
     CDS             3407..3823
                     /locus_tag="IV334_002489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-packaging protein"
                     /protein_id="HAP8944517.1"
                     /translation="MIPRFRAWDKRKNVMRDVAVLHFTKNGKTNFIEYWINPTELKSY
                     HVRNIELMQSTGLKDKNGIEIFEWDIVSVSVRNGFDYLDNKVCVVKNSIGHSGLVCAT
                     VDEDLEYQIFNTELFEEYTYEVIGNIWENSELLEEQ"
     gene            3823..4491
                     /locus_tag="IV334_002490"
     CDS             3823..4491
                     /locus_tag="IV334_002490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1642 domain-containing protein"
                     /protein_id="HAP8944518.1"
                     /translation="MNKQELIDELAKYVKRYENVMDEHGQGRYGAYEVSLKLVKRLNE
                     SKITDEQAWNKVAEAYPESAQSLRNTLDNAVFGKTGEPQKHVMPKFVAEWIEYAKKKG
                     DSLAISFKPWNLYGVEYSKADRWIGDNQETFARAWLDGYEVEKEPLYYVPLPYEVWDE
                     EAAELKTEYLYLHYEITSDETRIFPTKEPRKGFVAKLDELTIKSADENYWPFAVPVEE
                     VVEG"
     gene            4488..4865
                     /locus_tag="IV334_002491"
     CDS             4488..4865
                     /locus_tag="IV334_002491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8944519.1"
                     /translation="MKKLHQLISEKESELQNLEDSLGLGFPIVEQVKMVQISHLQLEL
                     EDLRQIEDPYQLNDNQQIVLEWLKLTASTGKPMQVVFWMMNNAAWGHLDELRDPLMEL
                     TDKQQFEVLTAFAQWGLEQEEKE"
     gene            4862..5161
                     /locus_tag="IV334_002492"
     CDS             4862..5161
                     /locus_tag="IV334_002492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8944520.1"
                     /translation="MKLKDGFYASSHGIGGLMLDMPTKNPKTRKKPKVKVGDMVRCEA
                     EGFIYPFRGYVEHVYNHSAIIRIENTMECDKLLAKSKDNLAVVRLVDMEVINNEI"
     gene            5403..5819
                     /locus_tag="IV334_002493"
     CDS             5403..5819
                     /locus_tag="IV334_002493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autolysin"
                     /protein_id="HAP8944521.1"
                     /translation="MQLLREVDFKQTRCNARDVLKNFRRLERMAGRSLIDIKSPIITD
                     MPKAPKHGNKAEDAIIQMMDIEAERDAILAALMALSLISRQILYYSFCDVNKHSNYEI
                     GQLIRGYGEKNVEKLKSIALIEFAEAYKKGVLVQYR"
BASE COUNT         2231 a          899 c         1391 g         1481 t
ORIGIN      
        1 aaataagcct caaagatgaa tcgaatacga ctgtgagtga tgctaatgtc ttagcaaata
       61 tcatcttaga tttcatcttt gaacatcata ttcctttcaa agaaggctat gagattttac
      121 cagcgaatca agaatattac ttctacaaat gcattacaaa aagagtttgt tgcatctgtg
      181 gcaaaacagg agctgacatc gatcactttg acaaagcgct aggaagacga aagcgaaaag
      241 aagttgatca ttcagagtac acatttgcag cactctgcag aatccatcac acggagaagc
      301 acaaaatagg tgtgatcaat tttaaaaata aatatcaaat caaaggaatc aagttaagtc
      361 atgaaacgat taaaaagtta aggataggag gataaatttg gctgagataa gttggatcaa
      421 acttaaaact actatgtttg acgatgaaaa aatacggtta atccaagctg ttcctgagtc
      481 ggatgccatc atcgttatat ggattcgatt actagtttta gcaggaaaga ctaacgacga
      541 tggtctgata tatatccaga ggaacatgcc ttataccgaa gaaatgcttg ctacattgtt
      601 tggcaaaaac gtaaatacgg ttcgcttagc gctaactaca ttggcaaatt tcaacatgat
      661 tgatctaagc agtgatggac taattgccat cagtaattgg gaaaaacatc aaaatatcga
      721 gggtatggat aaagtaaggc taaaaaatgc tgaaagaaac cgtaaataca gggaaagaaa
      781 gagacaggaa cgtctcaaat tggaaaatga cgttagcgtg acgtcacgtg acggtacaga
      841 taaagatata gaagaagata aagatataga taaagaagaa aagaaaggta agtattctga
      901 cgaacactta cgccttgcta aaaaaatgca aaggaattta actgaagatt ttccaaaaga
      961 aatgaacaaa gtagatatcg aaaaatgggc agacacaatc aggttgatgg aagaaagaga
     1021 taaagcatct atagaagcga ttgagtatgt gatcaattgg ctacctacaa atgaattttg
     1081 gtttggaaat attagaagtg ctaagaaatt gagagaaaaa tttgagaagc tcaaattcga
     1141 aatcaaagca gacaagaaga atcataaaaa gcaaagtcaa aaactacagt acagcaatcc
     1201 tagtgaatat gacgacttgc caatttaaaa ggagatgcat cacatggaaa gcctagcaaa
     1261 tgctatggag aagctaataa gaagagtatt agtgcaaagc ggaaaatgtc cagaatgtag
     1321 cgaacctttg tatagttggc gagctaaaaa taaggatggt tcagaacgtt gtaaaccaac
     1381 atgtatgagt tgtggttata aagcgttacg tgtgaaagag gatatacaga ccgaacggat
     1441 atataacgac agcttaaaag cacgagcatt gagttttttt caaaatggtt cggtattaac
     1501 agataaaact ttgtttaaat gcaaaatgga gaattatcac gtagtggacc aagaaacgaa
     1561 aattgcttta gaaagagcta aaagctatgt aaatgatgtc ctactgaacc atcctgcaca
     1621 tttcattcta tcagggaaat caggaagcgg aaaaagccac ttatcaatgg cgacagcttg
     1681 ggaaatactt gagcgctcaa attatgacaa gaaaatactt tttataagct atcaagagtt
     1741 attagagcaa ataaagtttt cttataacaa tgctgaactg agaaaagaaa ttgaaggatc
     1801 gcttatagcc gatatcaaaa caactgattt ggtggttttt gacgatattg gagctgaatt
     1861 aggtagcggg gtatcaaata gtaggcaatt tacaaacaac acgttaaaca cgctcttaga
     1921 agccagacag aacaaggcaa cgatcatcac aacaaactta tctggtcctg aactaagaga
     1981 agcctacggt gaaagaattg tttctaggat atttaagaat tcagaaggtt atgcgctgaa
     2041 attccaacaa acagcagaca agcgcataaa accagtgaaa ggtagtatcg catgaataaa
     2101 taccgtaata aaaaaactgt tcatcgaggt atcaagtttg attctatcgc agaagcagag
     2161 tattacgatc tagccttgtg gcaagctgaa gcgaacggct ggaaagtaaa acttcaggaa
     2221 agatttgagc tgatgccgaa atttgaacta gacggaaaga agtatcgcaa gatcgagtat
     2281 attcccgact tcacatttta taaaaacggc aaactagtca aagtcgtaga tgttaaaggg
     2341 atgcagacaa aagatttcaa aatcaaagcg aaactcttct gcagtcaata tcgagtacca
     2401 ctgatcttag cgaagaaata tcggaatacg ttcaaggaag aacggtttta acgaggtgat
     2461 ccatcatgac aacagaagaa gtgattcaaa tgcgtattcg aagccttcag cgtgagattg
     2521 acgatctgta acggacaaag gcagtgatgg tcaatgaaac ggcgaggaag gcaatcgatt
     2581 tgcacataga aaatttaaga aggaaaatcc atcgattgga ggaatgagcg tggataagaa
     2641 agcagcaatg aaacgaatca tcgaactgac acattctgag aattggcaag aagacaaaga
     2701 aatagttgca gaagtccaaa agctcggtaa atcaatgtgg gctgaaaagc ctaaacggaa
     2761 aacgccgaga aaaattgcaa tctggcatgg tgatcgaatt ctagtaacag gtactgctga
     2821 acagttatct gaaattactg gattaagcaa aaacattatc tgggatagag ctaggagctt
     2881 atggattgat tcaaaaggac gacagtttag gtatgtggag gagaaataat gaacgaacta
     2941 atcacaaaag tagagcagtg ggctaaagac aagggactgg atcaagcgga tccaaaagca
     3001 caatttttga aagtagctga ggaattcgga gaaattgcat cggcaatggc aagaagtaat
     3061 gatgagctat ttaaagatag cgtaggagac gttatcgtca cgctgattat tctttccatg
     3121 caaaaagggg caaacataca agagtgttta gaaatggcgt ataacgaaat caaaggacgc
     3181 acaggaaaaa tggtagatgg tgtattcgtg aagtcgagtg atttggagga cagcgaatga
     3241 tgatgccaaa agaaatctat tcagatgcat taagaggtgc tagaaatcaa ttgaaaatgg
     3301 caaaaagagc atacgaaatt cggcccacaa tcgaaaatga acgcagagta aaagcaattc
     3361 gccgtagatg ttcaatttac ggcgagttac agaaggagga cagcaaatga taccgagatt
     3421 tcgagcttgg gataaacgaa agaacgtaat gagagatgta gccgtcttgc attttactaa
     3481 aaacggcaaa acaaacttta ttgaatattg gataaatcct accgaattga aatcatatca
     3541 cgtgcgaaac atcgagctca tgcaatcaac agggctgaaa gataagaatg gcattgagat
     3601 tttcgagtgg gatattgtat cagtcagcgt gcgaaatgga ttcgattatt tagataataa
     3661 agtttgtgtt gtcaaaaatt caataggaca ttcgggatta gtgtgtgcca ctgttgatga
     3721 agatttagag tatcaaattt ttaacacaga gctgtttgaa gaatacacgt atgaagtcat
     3781 cggaaatatt tgggagaata gcgagttatt ggaggaacag tgatgaataa acaggaattg
     3841 attgatgaat tagctaaata tgtaaagcgt tatgagaacg ttatggatga gcatggccaa
     3901 ggaaggtacg gcgcttatga agtatcttta aagttggtga aaagactaaa tgaatcaaaa
     3961 attacggacg aacaagcttg gaataaggta gctgaggctt atcctgaatc ggcacaaagc
     4021 ttgagaaaca ctttagataa tgctgtattt ggtaagactg gtgaaccgca gaagcacgtt
     4081 atgccgaagt ttgtggcgga gtggatagaa tacgctaaaa agaaaggtga tagtctagct
     4141 atttcattca agccgtggaa cctctatggt gttgagtata gcaaggctga tagatggatt
     4201 ggagataatc aagaaacgtt tgctcgtgct tggctagacg gctacgaggt cgagaaagag
     4261 ccgttgtatt atgtcccgct accatatgaa gtttgggatg aagaggcagc ggagcttaaa
     4321 acagagtatt tgtatctaca ttatgagatc acaagtgatg aaactagaat atttcctaca
     4381 aaagaaccta gaaaaggttt tgtcgcaaaa ttagatgaac ttacgattaa atcagcagat
     4441 gaaaactact ggccgtttgc tgtaccagtg gaagaggtgg tagaaggatg aaaaaattgc
     4501 accaactcat atctgagaaa gaaagcgaac tccaaaattt agaagatagt ttgggattgg
     4561 ggtttccaat tgttgagcaa gttaaaatgg ttcaaatcag tcatctacaa cttgaattgg
     4621 aagatttaag acaaatagaa gatccgtatc aactcaacga taaccagcaa atcgtgttgg
     4681 agtggttgaa actaactgcg tcaacaggaa agccaatgca agttgtattt tggatgatga
     4741 ataatgcagc atgggggcat ttagacgaat tgagggatcc tttgatggaa ttaactgaca
     4801 aacagcaatt cgaagtccta acagcattcg ctcaatgggg attagaacag gaggaaaaag
     4861 aatgaaacta aaagacggat tttacgctag tagccacggt atcggcggtt taatgctaga
     4921 catgccgaca aagaacccta aaacacgtaa gaagccaaaa gtcaaagtcg gtgacatggt
     4981 tcgctgtgaa gcagaaggat tcatctatcc atttcgtgga tatgtagaac acgtctataa
     5041 tcactcagcg atcattcgca ttgaaaacac gatggaatgc gacaagttat tagcgaaaag
     5101 caaagataat ttagcagtgg tgagattggt ggatatggag gttataaaca atgaaattta
     5161 aaatctttga agaggacact cgctataaat tagaaaaaaa attaaatgaa tttgcaaaga
     5221 ataatgaaat tcagcatata tctttaacgg cttctaagtc aggttataca acttactatg
     5281 cagctgttgt gagctacgta attcaataaa aggaactcgg caaataaaaa agccggatca
     5341 ctccgactga tgtaataaat ccgacaagtt tattatatca cataaaggag cggtttgact
     5401 tgatgcaatt gttacgagag gtagatttca aacagacaag atgtaatgcg agagatgtgc
     5461 tgaagaactt tcggcgcttg gagcggatgg caggtcgctc tttgatagat attaagtcgc
     5521 cgattattac ggatatgccg aaggcaccga agcacggcaa taaggcagaa gacgcgatca
     5581 ttcagatgat ggatatagaa gcggagagag acgcgatttt agcggctttg atggcgctta
     5641 gtctgattag ccgtcagata ctctactaca gcttctgtga cgtaaacaag cactctaatt
     5701 atgaaatagg gcaattgata cgaggatacg gagagaagaa tgtagagaag ctgaaatcca
     5761 tcgcattgat cgaatttgct gaagcataca aaaaaggcgt gttagttcag tatcgttaat
     5821 tttgtagggt ttttgtaggg atagtgtagg gtttttgagc ggtttaacgt gatattatga
     5881 tagtgtcgaa agattagtga taggtctaag acaaaataat aataaaagga acatcgtttt
     5941 attattgttt cacaattaag cttcgataga cagcagcgga aatattaaga ataaggatgt
     6001 ga
//
LOCUS       DACDKM010000127         5988 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20834_2016
            SAMN12640370-rid9891663.denovo.127, whole genome shotgun sequence.
ACCESSION   DACDKM010000127 DACDKM010000000
VERSION     DACDKM010000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640370
            Sequence Read Archive: SRR10040891
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5988)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5988)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742835.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742835.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:04:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,012
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 29 of 75
            Pseudo Genes (incomplete)         :: 33 of 75
            Pseudo Genes (internal stop)      :: 19 of 75
            Pseudo Genes (multiple problems)  :: 5 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5988
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640370-rid9891663.denovo.127"
                     /isolate="E20834_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(<1..607)
                     /locus_tag="IV334_002494"
     CDS             complement(<1..607)
                     /locus_tag="IV334_002494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M protein trans-acting positive regulator"
                     /protein_id="HAP8944522.1"
                     /translation="MQTQEAIKTFLLKKKSNTKLDIFLFLSEHRFFITTQYLADHFHM
                     SESNFLLYIKELEQDFERLNLTELHIDKQKPFLKLNFEGIDPAYCYYRLFGRYCNESV
                     SYQILTSLFSCQTNSIISLSQQTNYSASYLYTKMKKINAFLALYGLSISFSNNGRKSF
                     SGKEVQIQYAALDIYWNIFSSTATEFYDEDPQVVAFMMNTFF"
     gene            755..2043
                     /locus_tag="IV334_002495"
                     /pseudo
     CDS             755..2043
                     /locus_tag="IV334_002495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318369.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
     gene            complement(2169..3248)
                     /locus_tag="IV334_002496"
     CDS             complement(2169..3248)
                     /locus_tag="IV334_002496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HAP8944523.1"
                     /translation="MNNVFIFCYLEVVDLTVYQRFLANEKIRRLTVLLFVVFVLFLVR
                     SMITTILLTFIFTYLMIHLVQIIQRFVRIPTGVLAIMIYTLIVYLIYLAFTKYIPMLI
                     HQTFDMIQSVINFYEHQPKDADPVLQYIHTYIERNDFFEQLQNGASIALGYLQDIGKM
                     AVAFAMSFILSFFFMIEKKKTIVFSRLFLKSEFSWFFQDIYYFADKFVNTFGLVLEAQ
                     FVVALLNTLLTTIALAVFGFHQLLSLAIMIFILSLIPVAGVIISCIPLSFIAYSQGGI
                     RDVVYILITILIVHLIESYVLNPKLMSSKTELPIFYTFVVLLVSERFFGVWGLIVGIP
                     IFTFFLDVLKVKKIPNHLPKETDIP"
     gene            complement(3351..3791)
                     /locus_tag="IV334_002497"
     CDS             complement(3351..3791)
                     /locus_tag="IV334_002497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAP8944524.1"
                     /translation="MEDLYLVNQVCFPLYAASKEIIRHYTPLLKPLNLTYTQYLVMLV
                     LWEKKKTSVKELGNDLCLDSGTLTPLLKKLAEKNYINRQRETGDERIVNVTLTAQGAA
                     LQKQAEGIPQKIKSYLPLEQEELDTLQHLTRKMLDNFHQDQKKG"
     gene            complement(3848..4258)
                     /locus_tag="IV334_002498"
     CDS             complement(3848..4258)
                     /locus_tag="IV334_002498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HAP8944525.1"
                     /translation="MKKMYSTKMINTGGRSGEVHNPDRTFELNIAAPGKRVENATNPE
                     QLFAAGFSACFNSALELIKQQKNISGASTVSAQVSLYSESETSFVLGVEIEGSIEGLS
                     LEETQELLEAAHKVCPYSKATAGNIEVTLKAVSE"
     gene            complement(4438..4872)
                     /locus_tag="IV334_002499"
     CDS             complement(4438..4872)
                     /locus_tag="IV334_002499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HAP8944526.1"
                     /translation="MHKGTEIDFVLNDSLKALELYQTIFDEIQLIEATDYPRGNNEVI
                     FTLYGTRFHMLDENTSLGLTAPTAEHPNTIWFNIIVSDIEDTHNKALRNGCVEIQEMT
                     EMMDFGVTNSMFRDPFGYLWMLHQVHREVIFEERKQLFEKYM"
     gene            complement(4972..5820)
                     /locus_tag="IV334_002500"
     CDS             complement(4972..5820)
                     /locus_tag="IV334_002500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HAP8944527.1"
                     /translation="MKETRQFIWTYIKIIFALFILGVAINMFLGPHHIAAGGVSGLGI
                     LLEAALGFDRAIVILVLNIVMLVLALLFLGKKPFFKVLFGSLVFPLIIAIVPETMITS
                     DRLLSVIFGSAIFALGVAILYKNNSSSGGTTIPPLIFKKYFHLNTSIGLFFTDAFVVS
                     LNLFIFGIEEFLYAILSIAITSIVMTYIETGINRKKSVMVMSEHYLEDIRLRLSAEIG
                     RGLTLLEAKGGYNRKSKEVLLIVVTDHEFSRMKPLIEEIDPSAFVIVSSVAEVMGRGF
                     TYHPIE"
BASE COUNT         2193 a         1119 c          987 g         1689 t
ORIGIN      
        1 taaaaaaagt attcatcata aaagccacta cttgcggatc ttcatcataa aattctgtag
       61 cagtacttga aaagatgttc caataaatat ctaatgctgc atattgaatt tgtacctctt
      121 ttccagaaaa agactttctg ccattattag aaaaggagat agacaaaccg tacaatgcta
      181 aaaatgcatt aatctttttc attttcgtat acaaataaga ggcactatag tttgtttgct
      241 gacttaaact gataatcgaa tttgtctgac agctgaacaa agaagtcaag atctgataac
      301 tgactgattc gttgcagtat cttccaaata gacgataata gcagtaagcc ggatcaattc
      361 cttcaaaatt caactttaaa aaaggttttt gcttatcaat atgcagttct gttagattga
      421 gacgctcaaa gtcttgttct aattctttga tgtatagtaa aaaatttgat tcagacatat
      481 ggaaatgatc tgctaaatat tgtgtagtaa taaagaaacg atgttcagat aggaaaagaa
      541 aaatatctaa ttttgtattg ctttttttct tcaagagaaa cgttttaatc gcttcttgag
      601 tttgcatgaa agaggctcct ttttacttaa attgttaaaa cttgaaaata ttactgtttt
      661 tttcataaat ttagacaaaa aaacagtaaa catataggta gaatctctac tatcttattt
      721 cttgcataca aaaaaatcaa ggagtgagag agttatgaga tattcaacca tcttaatatc
      781 aacatttgca gccttaagct tatcgcttac tataaatcag acagtccaca cagaggagat
      841 agaaacagcc aaatggacag aaaccaagtg ggccaacgtc aatcgaatta cctttcagga
      901 tgaattttca tccttcaatt caggagtcgc aaaattaaga aaaggacaaa cagcgaccat
      961 caaagcaaaa attgactatt caggagactt agcacctata ctacaatcag ctgttacatt
     1021 tgagaatgtt gatcccgctt tgtcgattgg ctatgacgag aacactttga ctatccaaca
     1081 caagcaagcg atatttacct tcaccgttac attaaatcag gacttgttaa aaccagcgct
     1141 ttttactgtc aaggtatcag atggactacc gaacgatcat catcatttaa cgccgtatag
     1201 tcagcggcag acaattgagc aagattcagc tatcgactca ggaggagatc ttgttgaaga
     1261 accaacagat aaaccagaaa atgaaaataa gccagaagta ccgcctacag aaaatccaga
     1321 cggagaacag aaaccggaaa tagaacctgg agaagaaccc gatacagaaa ctcaaccaga
     1381 gccagacaat gaatcaaaac ctgaaataac ccctggagaa aaaccagatg tagacccaga
     1441 agaaaaacca gatgtgactc cagaaccaga tacggattct ggaaaccaaa cagtaccgga
     1501 aacaaatcca gacacagata atgaaactga aaatccagaa aaaccagagg tagccccaga
     1561 agaaaaacca gatgtaactc cagaaccaga tacggattct ggaaaccaaa cagtaccgga
     1621 aacaaatcca gacacagata atgaaactga aaatccagaa aaaccagagg tagatccaga
     1681 agaaaaacca gatgtgactc cagaaccaga tacggacgca agagaccaag gaataccaga
     1741 aaaaataaac aaaaaaacaa ttcaagagga tggaaaaaaa gagagtaaaa aatcaaatct
     1801 agcaattcta aaaataaatg aagagcagct gaataaaaaa tcacgaatat ttgatagcgc
     1861 ccaatcagct gaaaccttaa aaagtagcaa ggatacaacc tttgctgtcc tgaaacgaaa
     1921 aataaacagc taccaaaatc cggagagtca caaaacaaag ttatactgtg gagcggaatc
     1981 attctattaa gtatagcaac aatgctttct gcaaaaagat ttaaacaaaa cagatctctc
     2041 taacatttga ataatgtctg tgaacgcatt tttcaaataa aaagaaatat ccagaaaatg
     2101 accccaaaat accagattga aatctgatat ttggggtcgt tcatacttta tctttcttca
     2161 tatttatttt aagggatatc tgtttctttt ggaagatggt tcgggatctt tttcactttc
     2221 aatacatcta aaaagaaggt aaagatcgga atgccgacaa tcaagcccca aacgccaaag
     2281 aaccgttcgc taacaagtaa gaccacaaat gtataaaaaa tcggcagttc tgttttgctg
     2341 gacatcaact tcggattcag cacataagac tcaattagat ggacaatcaa aatcgtaatc
     2401 aggatataaa caacatctcg gatgcctcct tgggaataag ctatgaaaga tagaggaata
     2461 caagaaatga taacacctgc tactgggatc agacttaaaa tgaaaatcat gatcgctaaa
     2521 ctcagcagct gatgaaagcc aaatacagcc aaggcgattg tagtgagcaa cgtatttaaa
     2581 agagcgacaa caaattgagc ttctaatacc aaaccaaatg tattgacaaa cttatcggca
     2641 aaatagtaaa tatcttgaaa gaaccaagaa aattcacttt ttaaaaacaa tcttgagaat
     2701 acaattgttt ttttcttttc aatcataaaa aagaaactta agatgaatga catagcaaac
     2761 gccactgcca tttttcctat atcctgtaaa tagccaagcg caatcgaagc gccattttga
     2821 agctgttcaa aaaaatcgtt tcgctcaata taagtatgga tatattgtaa aacaggatct
     2881 gcatcttttg gctgatgctc gtaaaaatta ataacggact ggatcatgtc aaatgtttga
     2941 tgaataagca tcgggatata ctttgtgaat gccagataga taaggtaaac gatcagtgta
     3001 taaatcatga tagctaaaac acctgtaggg attcggacaa atcgctgaat gatttgaaca
     3061 agatgaatca tcaaataagt aaagataaat gtgagcaaaa tggttgtaat catacttcta
     3121 actaagaaaa gaacaaaaac gacaaataat aaaacagtga gacggcgtat tttttcattc
     3181 gccaaaaaac gctgataaac ggtcaaatcg acaacctcca aataacaaaa aataaacacg
     3241 ttattcatta tatgatggaa tatgaaaata agaaagagca aaaagaaaaa ccgctaaatg
     3301 acgttatctt atgagttggt tttcctcata aaatcgttca tctagcagtt tcagcccttt
     3361 ttctggtctt gatgaaaatt atctagcatt tttcgggtca gatgttgcag tgtatccaat
     3421 tcttcctgtt caagtggaag ataagacttg atcttttgtg gaataccttc tgcttgtttt
     3481 tgcaacgcag caccttgagc agtcagcgta acatttacga ttctttcatc acccgtttca
     3541 cgctgacgat taatataatt tttttctgct aactttttta gtaagggagt cagcgtacca
     3601 gaatccagac acaaatcatt tcctagctct ttcactgaag tctttttctt ttcccataaa
     3661 actaacatca caagatactg ggtataggta agattcagcg gcttgaggag gggagtgtag
     3721 tgtctaatga tttctttaga ggcggcatat aaaggaaaac atacttgatt aactaaatat
     3781 agatcttcca tttttccacc tccgctgttt tactatagtg tatcatagcg gatttacaca
     3841 aaaaagtcta ttcactaact gctttcagag taacttctat atttccagct gtagcttttg
     3901 aatacggaca cactttatgc gctgcctcta ataattcttg cgtttcttcc agagaaagac
     3961 cttcgataga tccttcaatc tctacaccta agacaaagct cgtttcactt tctgaataaa
     4021 gggaaacttg tgcactgaca gtagaagccc cagagatatt tttttgctgt ttgatcaatt
     4081 ctaatgcact gttaaaacat gcactgaaac cagctgcaaa caattgttca gggttggttg
     4141 cattttcgac gcgtttacca ggagctgcaa tattcaattc gaatgtacga tcgggattat
     4201 gcacttctcc agagcgtccg cctgtgttga tcattttcgt agaatacatt tttttcataa
     4261 aattaccacc tttaattata ttttatacaa ttgaatttta cacaatataa ttgtggaaag
     4321 caaagaaatg gattggcata agataaataa gagaaagcac atgaaacaga cgcgctttct
     4381 cgaatagcaa acatatacaa aaaatatcag agacaaaaaa taatctcatc ataaacatta
     4441 catatatttc tcaaaaagtt gttttctttc ttcaaaaatt acttcacgat ggacctgatg
     4501 aagcatccat aaataaccaa aaggatctct gaacattgag ttagtcacac caaagtccat
     4561 catttctgtc atttcttgaa tctctacaca gccatttcgt aatgctttat tatgtgtgtc
     4621 ctcgatatca gagacgatga tattgaacca gatcgtattt ggatgttcag cagtaggagc
     4681 agtcaaacca agagacgtgt tttcatccag catatggaat ctcgtaccat acaaggtaaa
     4741 aatgacttca ttattgcctc tagggtaatc tgttgcctca atcagttgta tttcatcaaa
     4801 gattgtttga tacagttcta gtgctttcaa actatcattt aatacaaaat cgatttccgt
     4861 tcctttatgc ataaagatct cctctccttt tcagtcgaac agttactaga ttcttttatt
     4921 ataccagcta aagaaaacgc attcaaaaaa atagcggatt tacttaccaa ctcattcgat
     4981 tggatgataa gtaaatccgc gtcccattac ttctgctacg ctgctgacaa tgacaaaggc
     5041 acttggatcg atttcttcta tcaatggttt cattcgggaa aattcatgat cagtcacaac
     5101 gatcaataaa acctcttttg actttcgatt atacccgcct tttgcctcta gcagggtaag
     5161 gccacgtcca atctcagcac ttaagcgaag gcggatgtct tccaaataat gttcactcat
     5221 gaccataaca gatttctttc gattgatacc tgtttcgata taagtcatga caattgaagt
     5281 gattgcaata gataaaatag cataaagaaa ctcttctatt ccaaaaataa acagattaag
     5341 agaaacaacg aatgcgtctg taaaaaataa gccaatcgaa gtattcaagt ggaaatattt
     5401 tttaaaaatg aggggaggga tcgtcgtacc gccgctagaa gagttgtttt tatataaaat
     5461 cgcaactcct aaagcaaaaa tcgcacttcc aaaaatgaca gacaacaacc gatctgaagt
     5521 aatcattgtt tctggaacta ttgcaatgat taaaggaaaa accaagcttc cgaaaagtac
     5581 tttaaaaaag ggttttttac ctaaaaatag aagagctagt accaacataa cgatattcaa
     5641 cactaaaata actatagctc ggtcaaagcc taaagcagct tccagtaata ttcctagacc
     5701 gctgacacct ccagcagcaa tatgatgagg ccctaaaaac atattgatag cgactcctaa
     5761 tatgaagagg gcaaaaataa tcttaatgta tgtccatata aattgtctcg tttctttcat
     5821 ttattcactt ccttaattca ttaataatac tcttatctta ttataactta cttttcacct
     5881 taagttaact ttaattaata aaaaaattag gctagtctaa attattatgt ttcattcttc
     5941 tagaatatac ttctacgcca aaaactgtta aaaaactttt actttaga
//
LOCUS       DACDKM010000128         5974 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20834_2016
            SAMN12640370-rid9891663.denovo.128, whole genome shotgun sequence.
ACCESSION   DACDKM010000128 DACDKM010000000
VERSION     DACDKM010000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640370
            Sequence Read Archive: SRR10040891
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5974)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5974)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742835.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742835.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:04:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,012
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 29 of 75
            Pseudo Genes (incomplete)         :: 33 of 75
            Pseudo Genes (internal stop)      :: 19 of 75
            Pseudo Genes (multiple problems)  :: 5 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5974
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640370-rid9891663.denovo.128"
                     /isolate="E20834_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(170..3319)
                     /locus_tag="IV334_002501"
     CDS             complement(170..3319)
                     /locus_tag="IV334_002501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease subunit R"
                     /protein_id="HAP8944528.1"
                     /translation="MKISHREEAEVEEQLIRVLGEGHNQWTYRPDLKSEEDLWSNLRQ
                     KIISNNQAELNDSPLTDKEFETIKTELLLRTKTPFDAAKWLKGENGMARITIERENPQ
                     LGSVSLILYSNQDIGGGISTYEVVHQIAKRGSNIEARDRRFDVTLLINGLPIIQIELK
                     QVTAKDGFYQAFNQIKKYAEEGMFRNNIFSTLQLFVVSNEQTTRYFANALPKDLHPKF
                     LFSWRTKDNKKVENLYEFCKQVLNIPDAHRLIADYTIVSEDQDNKTLMVLHPYQVHAI
                     QALFIAANKHQSGYVWHATGSGKTLTSFVSTKLLARKSGIDRTIMLVDRKDLDNQTTT
                     EFTKFASEFNTGVSSGNAKANSLIVGTGSAKELSETLLADANANVVIITTRQKLDAAL
                     KYAKKQEEKKGTNRFQKLMGQHIVFVVDECHRALSAENMEEIKKMFPKSTWFGFTGTP
                     IFPENRKQAKGQLARTTHDQYGEVLHTYTIKNALEDGSVLGFQVEHENTVEPTSLENK
                     IYRKLKEVETYAEYSPEQINRMIDQMESVKKESYLDPTVFEAEEHIQKVIHKMFRPDN
                     AYTKFDFRNGRPTKSAILTTSSIDMAKKYYRAIKEMTKESDWLIKEFPNQPIREGRTM
                     EDPDFPRIAITYSLEENSRDSSAQQEEMQKIIEEYNQYYDTAWSLADIERYNGDINNR
                     LARKRAEFKQFGKQIDLVIVVDRLLTGFDAPTIQTLFVDRNLEYAGLIQAFSRTNRTY
                     PEKTKGLIVTFRKPATMEKNVEDATKLYSEAKEESGLIYPSYQESKKRFKQAYGKLKE
                     VSSMESIDEHTPLEIRIEYVKAFQELNRAYESLVTYDDYNDDMVQSSTLNNQIQLLEE
                     QIGVYETVKGSLMEEEPEKEGEDFSTIEFYSENTTKLYDIDSTYIDQLLGTYAANSSD
                     AREEIEKALAKLNKTEGVKEVYRQILNAFDVGTLDSNEDIFVIKRRYFTQASDNLIHA
                     FSNEWFVSEKELHASNLQYMPGEDPIPNMKAIINSKDYEGYKAKHPEAKPFKYPQEMK
                     RAWRKMLDDELIPLENELR"
     gene            complement(3333..4550)
                     /locus_tag="IV334_002502"
     CDS             complement(3333..4550)
                     /locus_tag="IV334_002502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HAP8944529.1"
                     /translation="MSNDTRPEIRFPGFTEDWEERKLGDMMDVTSVKRIHQSDWTNSG
                     IRFLRARDIVSAAKNEEPSDYLYISEEKYNEYSKNSGKVSQGDLLVTGVGSIGVPLLI
                     TDDNPIYFKDGNIIWFKNEHKIDGNFFYYSFINNKIQKYIRDVAGIGTVGTYTIDSGK
                     KTPISLPTYDEQIKIGSFFKQLDHTIMLHQHELDLLKETKKGFLQKMFPKNGAKVPEV
                     RFPGFTGDWEERKLGEVVVFLNGRAFKQQELLDSGKYRVLRVGNFNTNDRWYYSNLEL
                     EEDKYANKGDLLYLWATSFGPEMWNEERVIYHYHIWKLKFLSKELDKQYLYTWLLTDK
                     EKIKESTNGTTMVHVTKGGIEQRTFQFPPDINEQQKIGAFFKQLDDTIALHQRKLDLL
                     KETKKGFLQKMFV"
     gene            complement(4540..>5974)
                     /locus_tag="IV334_002503"
     CDS             complement(4540..>5974)
                     /locus_tag="IV334_002503"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type I restriction-modification system subunit
                     M"
                     /protein_id="HAP8944530.1"
                     /translation="EEYDTVSKQTMLYRELLSDEESKEDLIATIVDILGYSISPEYLF
                     NVLADQAKQATFQLNDLNKAFVQLASTYNQFNGLFDDVDLQSKKLGTDEQQRNVTITE
                     VIKKLNDVEVLGHDGDVIGDAYEFLISQFASEAGKKAGEFYTPHMVSDMMAQIVTLDQ
                     KERRFFSVFDPTMGSGSLMLNVRNYLTHPDNVKYHGQELNTTTYNLAKMNLILHGVDA
                     EEMNLRNGDTLNKDWPTDEPYTFDAVVMNPPYSANWSADTTFLDDSRFNRYGKLAPKS
                     KADFAFLLHGFYHLKETGTMAIVLPHGVLFRGAAEGVIRQKLLEDGSIYAVIGMPANL
                     FFGTSIPTTVIVLKKNRQTRDVLFIDASREFVKGKNQNKLSEENIQKILETYAERKDV
                     EKYAHLATFDEIKENDYNLNIPRYVDTFEEEEPIDMVHVGNDIKKIRQEQQVLEKELL
                     EAISSLQTTPENEAWLQGALEVFKHEQ"
BASE COUNT         1630 a         1269 c          998 g         2077 t
ORIGIN      
        1 tgcaacagaa ccggtaaatg aatgaaaaag atggaagaat cattattcaa tagctttctt
       61 aaatactccg ttataaaatg tgaacaagta aaaagacatc tatccctatt ttctacactt
      121 ccactttact actatctttg aatcgtacaa ttttcttaac tcttctcgtt tacctcaatt
      181 cattttcaag tggaatcaac tcatcatcta acattttacg ccatgctcgc ttcatttctt
      241 gtgggtattt aaatggtttc gcttctggat gtttggcttt atatccttcg taatccttac
      301 tgttgataat tgctttcata ttaggaattg gatcttcacc tggcatatat tgcagattag
      361 aagcatgtaa ttctttctca gaaacaaacc actcattgga aaaggcatga atcaaattat
      421 cacttgcttg tgtaaaatag cgacgtttaa tgacaaagat atcttcatta ctatctaaag
      481 tccccacatc aaatgcattt aaaatttgtc ggtaaacttc tttgactcct tctgttttat
      541 tcaatttagc caaagctttt tcaatttctt ctcgtgcatc tgaactattc gcagcataag
      601 ttcctagtaa ttgatcaata taagtagaat caatatcata caacttagtg gtattttcac
      661 tataaaattc gatcgttgaa aaatcttctc cctctttttc tggttcttcc tccatcaatg
      721 aacccttcac ggtctcgtac acgccaattt gttcttccaa aagttgaatt tgattgttca
      781 aagttgaaga ttgcaccatg tcatcattgt aatcatcata agtcactaac gattcatacg
      841 cacgattcaa ttcttgaaat gctttgacgt actcgatacg gatttcaagt ggcgtatgtt
      901 catcaatgga ttccatggat gaaacctctt ttaacttacc atatgcttgc ttgaatcttt
      961 ttttggattc ttggtaactt ggataaatca aacccgattc ttctttagct tctgaataca
     1021 gttttgtcgc atcctcgaca ttcttctcca ttgtcgctgg ctttcggaaa gtaacaatta
     1081 gtccttttgt tttttcagga tacgtacggt tcgtacgtga aaaggcttgg atcaatcctg
     1141 catactctag attacgatcc acaaataggg tttgaatcgt aggggcgtca aaacctgtca
     1201 atagacgatc cactacgatt actaaatcga tttgtttccc aaattgtttg aattccgctc
     1261 tttttcgtgc caaacgatta ttgatgtctc cgttataacg ctcaatgtct gctagtgacc
     1321 aggcagtatc ataatactga ttatattcct caataatttt ttgcatttct tcttgttggg
     1381 cacttgaatc tcggctattt tcttctaatg aataggtaat agcgattcgt gggaaatcag
     1441 gatcctccat cgttcgtcct tcacggatag gctgattggg aaactctttt attaaccaat
     1501 cagattcttt cgtcatctct ttgatggccc gataatactt tttggccata tcaatggaac
     1561 tcgtggttaa gatcgcagac ttcgttggac ggccatttcg aaaatcaaat tttgtatagg
     1621 cattgtctgg acggaacatt ttatgaatga ctttttggat atgttcctct gcttcaaaaa
     1681 cagtagggtc caaataactt tcttttttga cagactccat ttgatcgatc atacgattga
     1741 tctgttccgg tgaatactct gcatatgttt ctacttcttt taatttccga tagattttat
     1801 tctctaatga agtcggctcc acggtgtttt catgctctac ttgaaatcct aaaaccgagc
     1861 catcttctaa tgcattcttg atggtatagg tgtgcaagac ctctccatac tgatcatggg
     1921 tcgtacgtgc caattgccct ttggcttgtt tccgattctc agggaaaatc ggtgtacctg
     1981 taaagccaaa ccaagtggat ttcggaaaca tttttttgat ttcttccata ttttcagcac
     2041 ttaacgctcg atgacactca tcaactacaa acacgatatg ttgccccatt aatttttgga
     2101 agcgattggt tccttttttc tcttcctgtt tctttgcata tttcaacgcc gcatctagtt
     2161 tttgacgggt tgtaatgatc acaacattcg cattggcatc tgccaacaga gtttcactta
     2221 actctttggc acttcctgtt cccacgatca agctattcgc tttggcatta ccagaagaaa
     2281 ccccagtatt aaactccgaa gcaaatttcg taaactctgt ggtggtctga ttatctaaat
     2341 ctttgcggtc cactaacatg atcgttcgat cgatccccga ttttcgtgct aaaagcttag
     2401 tagatacaaa actagtcaac gtctttcctg aaccagtcgc atgccacaca taccctgatt
     2461 gatgtttatt tgcggcaata aacaaggctt gaatcgcatg cacttgataa ggatgtaaca
     2521 ccatgagggt tttattgtct tgatcttcac tgacaatcgt ataatccgca atcaagcgat
     2581 gggcatctgg gatatttagc acttgtttgc aaaactcata taagttttct acctttttat
     2641 tatctttcgt tcgccaacta aataaaaact taggatgcaa atccttcggt aaggcatttg
     2701 caaaataccg agtcgtttgt tcattggaaa ccacaaacaa ctgtaaggtc gaaaagatat
     2761 tgttccgaaa catgccttct tcagcatatt ttttgatttg attaaacgcc tgataaaaac
     2821 catcttttgc agttacttgt tttagctcga tttggataat cggtaagcca ttgatcaata
     2881 aggtgacgtc aaaacgtcga tcccgcgctt ctatgttact tccacgtttt gcaatttgat
     2941 ggaccacttc ataagtagag attcctccac caatgtcttg attggaataa aggataagtg
     3001 agacagaacc caattgcgga ttttcccgtt caatcgtgat ccgagccatc ccattttcac
     3061 cttttaacca tttcgcagca tcaaatgggg ttttcgtccg tagcaataat tcggttttga
     3121 tcgtttcaaa ctctttatcg gtcaaaggag aatcattcag ttctgcttga ttatttgaga
     3181 tgattttttg acgtaaattg ctccaaagat cttcttctga ttttagatcc ggacgatacg
     3241 tccactgatt atgtccttcc ccgaggacac gaatcaactg ttcttcgact tccgcttcct
     3301 cacggtgact aattttcatt cttcttccct ccctaaacaa acatcttttg caagaagcct
     3361 tttttcgttt ccttcagcaa atctaactta cgttgatgaa gagcgatcgt atcatctagc
     3421 tgcttgaaga aggcaccaat tttttgttgt tcatttatat ccggtggaaa ctgaaatgtc
     3481 ctttgttcta taccaccttt agtcacatgt accattgtcg ttccgttagt tgattcttta
     3541 attttctctt tatcagtcaa tagccaagta tatagatact gtttgtccaa ttccttagat
     3601 aaaaatttaa gtttccaaat atgataatgg tagattacac gttcttcatt ccacatttca
     3661 ggaccgaagc ttgttgccca taaatataag agatctcctt tattagcata cttgtcttcc
     3721 tctaattcta agtttgaata ataccatcta tcattagtat taaaattacc tactcttaaa
     3781 accctatatt ttccagaatc aagtaattct tgttgtttaa aagctctacc attcaaaaaa
     3841 actaccactt cccccaactt ccgctcttcc caatctcccg taaatcctgg aaaacgaact
     3901 tctggcactt ttgctccgtt tttagggaac attttttgta agaatccttt tttcgtttcc
     3961 ttcagcaaat ctaattcgtg ctggtgaagc atgatggtgt gatcaagttg tttgaaaaat
     4021 gaaccgatct tgatctgttc gtcataagtt ggaagtgaga taggtgtctt tttaccacta
     4081 tcaattgtat aggttccgac ggttccaatg cctgcaacgt cacggatata cttctgaatt
     4141 ttgttattta tgaacgagta gtaaaagaag tttccgtcaa ttttatgctc attcttgaac
     4201 caaatgatgt ttccatcctt aaagtaaatt gggttatcat cagtgattaa taaggggaca
     4261 cctattgatc cgacaccagt tacaagcaag tctccttgag aaactttacc tgaatttttc
     4321 gaatattcat tatatttttc ttctgaaatg taaagataat cggatggttc ctcatttttt
     4381 gcagctgaga caatatcacg agcacgtaaa aatctaatac ctgagttagt ccaatctgat
     4441 tgatgaattc gcttcacaga agtaacatcc atcatatctc ctaacttacg ctcttcccaa
     4501 tcttccgtaa aaccaggaaa ccggatttct ggtcgtgtat cattgctcat gtttgaacac
     4561 ctccagcgca ccttgtaacc acgcttcatt ttcaggtgtt gtttgcaggg aagagatagc
     4621 ttctaaaagt tctttttcta gcacttgctg ttcttgtcgt atcttcttga tgtcatttcc
     4681 gacatgaacc atatcaattg gttcttcttc ttcgaaagta tcgacatagc gtgggatatt
     4741 taagttatag tcattttctt tgatttcatc aaaggttgcc aaatgagcgt atttctcgac
     4801 atcttttctt tcagcgtagg tttcgaggat tttttgaatg ttttcttccg ataatttatt
     4861 ctgatttttc cctttaacaa attcacgact agcatcaatg aataatacat ctcgagtttg
     4921 acgattcttc ttcaatacaa tcaccgtcgt tggaatcgat gtaccaaaga acaagttcgc
     4981 aggcataccg atcacagcat aaatactgcc gtcttctaat aatttttgac gaatgacccc
     5041 ttctgcagca ccacggaata aaaccccgtg tggcaagacg atagccatag tccctgtttc
     5101 cttcaaatga tagaaaccat gaagaagaaa cgcaaaatct gctttggatt ttggcgctaa
     5161 tttcccgtag cgattaaacc gagaatcatc taagaaggtc gtatctgctg accaattagc
     5221 cgaataaggt ggattcatca ctactgcatc aaatgtgtaa ggttcatctg ttggccagtc
     5281 cttattcaac gtatccccat tgcggagatt catttcttcg gcatctaccc catgtaaaat
     5341 caagttcatt tttgctagat tgtatgtcgt tgtatttagt tcttgcccat gatatttaac
     5401 gttatctgga tgagttaagt agttacgtac atttaacatc aaggaacctg aacccatggt
     5461 tgggtcaaag acactaaaga atcgtcgttc tttttgatcc aacgtcacga tttgtgccat
     5521 catatcagaa accatatgag gcgtgtaaaa ttctccggct ttcttccctg cttctgaagc
     5581 aaattgactg atcagaaatt catacgcatc cccgatcaca tccccatcgt gacctaaaac
     5641 ttccacgtca ttcaatttct tgataacttc ggtaatcgtc acatttcgtt gttgctcatc
     5701 tgttcctagt ttttttgatt ggagatccac atcatcaaat aacccattaa attgattgta
     5761 ggtagatgcc aattgcacaa aggctttgtt tagatcgttc aattggaacg ttgcttgttt
     5821 tgcttgatcc gctaagacgt tgaaaagata ctctggtgag atcgaatacc ccaaaatatc
     5881 aacgattgtg gcaatcaggt cttcttttga ttcttcatct gaaagtaatt cacgatacaa
     5941 cattgtttgt ttgcttactg tgtcatattc ctct
//
LOCUS       DACDKM010000129         5878 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20834_2016
            SAMN12640370-rid9891663.denovo.129, whole genome shotgun sequence.
ACCESSION   DACDKM010000129 DACDKM010000000
VERSION     DACDKM010000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640370
            Sequence Read Archive: SRR10040891
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5878)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5878)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742835.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742835.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:04:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,012
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 29 of 75
            Pseudo Genes (incomplete)         :: 33 of 75
            Pseudo Genes (internal stop)      :: 19 of 75
            Pseudo Genes (multiple problems)  :: 5 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5878
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640370-rid9891663.denovo.129"
                     /isolate="E20834_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(45..1511)
                     /locus_tag="IV334_002504"
     CDS             complement(45..1511)
                     /locus_tag="IV334_002504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide MFS transporter"
                     /protein_id="HAP8944531.1"
                     /translation="MEKQQDKGFFGQPKGLSTLFFTEMWERFSYYGMRALLIYYMYDT
                     VANGGLGLPRATALAIMSIYGSLIYMSSIVGGWVSDRVLGAKRTVFFGGVFIMLGHIV
                     LALPFGVSTLFVSMALIIIGTAFLKPNVSGMVGHLYSKTDLRRDAGFSIFYMGVNLGA
                     LLAPLIVGTIGQEYNYHLGFSLAAIGMFFGLLQYIIQGRKTLDGVGTTPPNPLTTEER
                     KGFIRNLIIALVVIAAVFGGAQLTGHLTIDFFVNTISVLGIILPLYYFIKMLTSKDVT
                     AEEKPRVWAYIPLFLAAIIFWSLQEQGSSILALFASERTQSTLFGVTIQASWYQSLNP
                     IFIFILTPVFVTMWTKLGERQPSTVVKFSLGLLFAGLSYLLLMLPGMLYGTSGKVSPL
                     WLLGSFFIVVIAELCLSPVGLSVTTKLAPRAFESQTIAIWFLADASSQAINAQIARFY
                     TPGTESAYFGIIGAVAIVGGILLFTVKEPIKKLMGSIH"
     gene            1898..2494
                     /locus_tag="IV334_002505"
     CDS             1898..2494
                     /locus_tag="IV334_002505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HAP8944532.1"
                     /translation="MTTFTDTLKNRRSIYHLGRNVSLSNEELTTLIKEAIKESPTAFN
                     AQSTRAVILFGDAHEKLWEITEEALRPLTPAEAFPNTQNKLAGFKNGYGTVLFFKDTD
                     VVKGLQEQFELYADNFPDWSEQSNGIATANTWVALVDKGLGANLQHYNPVIDEAVAKE
                     WNIPSNWKLRSQLVFGSPETPAGEKEYMNDADRFRVFG"
     gene            complement(2552..3034)
                     /locus_tag="IV334_002506"
     CDS             complement(2552..3034)
                     /locus_tag="IV334_002506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8944533.1"
                     /translation="MVTFFTVLLFLFVVVMFLFLWLVRKEIIYRTVRNRWVYLVIPFL
                     VSLVIWYTLISHPTADELAKGILSAMIFISFLLDSRGITEEGLVLNSFDKKGVPFSEI
                     NKIVLYQPKGSKIVKMNFFRNGWRGPMLKFSASLEELVPFLSQRLNDEAEIDIMIDPE
                     "
     gene            complement(3140..3337)
                     /locus_tag="IV334_002507"
     CDS             complement(3140..3337)
                     /locus_tag="IV334_002507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8944534.1"
                     /translation="MTEEMINLGEQYACKPIGFTKTVIGEVVSKMTNCAVVKVAQCAA
                     EDQELLDEKASMVVAKYDTFE"
     gene            3632..4390
                     /locus_tag="IV334_002508"
     CDS             3632..4390
                     /locus_tag="IV334_002508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase C39"
                     /protein_id="HAP8944535.1"
                     /translation="MKKKTFKKLLFLGGTAALLLSGTSYYLHIHSPEHTMNTAQKSST
                     QKTKKTKEKAEVLLDVPLESQFDSPSLENGCEVTSLSMLLSFYGYETTKNQLAEQLDY
                     VPVFNADGTHGDPNEGFVGDISGGDWAMVVYVPPVASLAQQIVQTDYHTFPKTDAQLD
                     DIKKALSKGNPVWTSVTINFEVPDETDFMTWTTNNGEVRVTPLVHACVVTGYDKENIY
                     VNDPYGVKNRAVPINDFSAIFTAMGGQMLTLEKS"
     gene            4450..4878
                     /locus_tag="IV334_002509"
     CDS             4450..4878
                     /locus_tag="IV334_002509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8944536.1"
                     /translation="MEKFTHKKMDPNEIPIIFVRDCKGNVQGKVSINEWNERRRPATL
                     NELEIKLYRQSLVYYADQEYEKATDLLKFLIARTEYTRFEYIERLANIYHIMNEPVKE
                     YQLLDTVLSVAELIALPAGLEKKLVRRLLRVKQQLSDQEK"
     gene            4924..4996
                     /locus_tag="IV334_002510"
     tRNA            4924..4996
                     /locus_tag="IV334_002510"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4957..4959,aa:Gln,seq:ctg)
     gene            complement(5146..5517)
                     /locus_tag="IV334_002511"
     CDS             complement(5146..5517)
                     /locus_tag="IV334_002511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HAP8944537.1"
                     /translation="MKVIKYLIGLAAISGILLFGLKYYTEGSYGEIPGILDQLNPLVE
                     KGEVYVKTQKPEEVNEYGTARYVQKAADANGKERTVEFNGLSVLKENHYLKISNKGAH
                     IETYEEVPREKVPEKALAIIG"
BASE COUNT         1979 a         1140 c          976 g         1783 t
ORIGIN      
        1 aggaactgct gcaaataagc aattcctgtt tttatgttca ttgattaatg aatagagccc
       61 attagttttt tgattggttc tttcacagta aatagcaaga taccaccgac gattgctact
      121 gcaccgataa taccgaagta agcagattct gttcccggtg tgtagaagcg tgcaatttgt
      181 gcattgattg cttgtgaaga agcatcagct aagaaccaaa tagcgattgt ttgtgattca
      241 aatgctcgtg gtgctaattt tgttgtcaca gacaaaccaa ctggtgaaag acacaattca
      301 gcaataacca cgatgaagaa acttcccaac aaccacaatg gacttacttt tccacttgta
      361 ccataaagca tacccggcaa catcaatagc aagtacgata atcctgcaaa taaaagtcca
      421 agagagaatt tcactactgt agatggttga cgttcaccaa gtttcgtcca catcgttacg
      481 aatactggtg tcagaataaa gatgaaaatt ggattcaatg attgatacca gcttgcttgg
      541 atcgttacac caaatagcgt agattgtgta cgttcactag caaataaagc taagatagat
      601 gaaccttgtt cttgtaatga ccagaaaatg attgctgcaa ggaataatgg gatataagcc
      661 catactcttg gtttttcttc agcagttaca tcttttgaag tcagcatttt gataaagtaa
      721 tacaacggta agataatccc caatacactg attgtattga caaaaaagtc aattgtcaga
      781 tgaccagtta gttgtgcccc gccgaatact gcagcgataa caacaagtgc gatgatcaag
      841 ttacggatga atcctttacg ttcttcagtt gtcaaaggat tcggcggtgt tgtacccact
      901 ccgtctaatg tcttgcgtcc ttggatgatg tattgtaata atccaaagaa catcccaata
      961 gcagctaaag agaaccctaa atgatagtta tattcttgac cgattgtacc aacgattagt
     1021 ggtgctaata atgcccctaa gttgacaccc atgtaaaaaa tcgaaaatcc tgcatctctt
     1081 cgcaagtcag tctttgaata aagatgtccg accatgccag aaacatttgg tttcaaaaag
     1141 gcggttccga taataatcaa tgccatcgaa acaaacaatg tacttacacc aaatggtaaa
     1201 gccaagacga tgtggccaag cataatgaaa actccaccga agaatacagt gcgttttgct
     1261 cctaacactc ggtcagatac ccatccgccg acgatactag acatgtagat caaagatccg
     1321 tagatcgaca tgatagctaa tgctgttgct cgtggtaatc caagtccccc gttggcaact
     1381 gtatcgtaca tataatagat aagtaatgca cgcataccgt aataactaaa tcgttcccac
     1441 atctcagtga agaacaaagt tgacaatccc ttcggctgcc cgaagaagcc cttgtcttgc
     1501 tgtttttcca taatctatac cccaattctt taaattttca gaaaatatta tacatcttta
     1561 ccgaaaaatt taactaaaaa acttatttaa gaaatataaa ctaattttct ataaaagcaa
     1621 gtaattttta atggcaatat cataaaaaaa agaagataga gatttaataa ttatatatca
     1681 gctgtttttt tcaaataagc aaaaattcct aaaacacaac aatgaaagcg ataactaaga
     1741 aaatccttac ataacgattt ttcttattaa aattgttttt tgaacttcgc ttattttttg
     1801 tgagcatatc tgtttactta ctttttgtaa ggtgctatac ttcatttcgt aatcaattaa
     1861 aaaaatgcaa aacgcaaatc aaaggagaaa ttttattatg acaactttta ctgacacttt
     1921 gaaaaaccgc cgttcaatct atcatctagg acgtaatgtt tcactttcta atgaagaatt
     1981 aacaactttg atcaaagaag caattaaaga aagcccaact gctttcaatg ctcaatcaac
     2041 acgcgcagtt atccttttcg gagatgcaca tgaaaaactt tgggaaatca ctgaagaagc
     2101 acttcgccca ttgactccag cagaagcttt cccaaataca caaaacaaat tagctggttt
     2161 caaaaatggt tatggtacag tattattctt caaagatact gacgtggtaa aaggtctaca
     2221 agaacaattt gaattatatg cagacaactt cccagattgg tcagaacaat caaacggaat
     2281 cgcaacagct aatacttggg tagcattagt tgataaagga ttgggcgcaa acttgcaaca
     2341 ttacaaccca gttattgacg aagcagttgc aaaagaatgg aatatccctt caaactggaa
     2401 actacgttcg caattagtat tcggttcacc agaaacaccg gctggggaaa aagaatacat
     2461 gaacgatgcc gatcgcttcc gcgtatttgg ataattttat ttgaaataaa aaacagaggt
     2521 tgtgacaaac atcttgtcgc aacctctgtt tttattctgg atcaatcata atatcaatct
     2581 ctgcctcatc gtttaagcgt tgggacaaaa atggaactaa ttcttctaat gatgcagaaa
     2641 atttcagcat cggtcctcgc catccgtttc ggaaaaaatt catttttacg attttagaac
     2701 cttttggctg atataataca attttattga tctcagaaaa aggaacacct tttttgtcaa
     2761 agctattcaa aacaagtcct tcttctgtga ttcctctact gtccaacaaa aaactaataa
     2821 aaatcattgc tgacaagatc ccttttgcca attcatctgc tgtaggatga ctgatgagtg
     2881 tataccaaat gactagtgac actaagaagg gtatcaccaa gtagacccat cgatttcgca
     2941 cagtccgata aataatttct tttcgaacca gccaaagaaa taaaaacatc acaacaacga
     3001 ataaaaacaa tagaaccgta aagaatgtta ccatgacaac tcgcctcgct ttttcttctc
     3061 cagaaaatag gaaaagaata ctgatttacc ttctcaaaga aattggactt gcagcacgcc
     3121 gcacgtccaa ttgttttctt tattcaaatg tatcatattt tgcaacaacc atgcttgctt
     3181 tttcatctaa taattcttgg tcctctgcag cacattgggc aactttgacc acggcgcagt
     3241 ttgtcatttt tgatacaact tcaccaatga ccgtttttgt aaaaccaatt ggtttgcatg
     3301 catattgttc tcctaaatta atcatttctt ccgtcatcaa aatcctccta aactgctgga
     3361 acttttctcc acattaccac tgctatttct ttggagattt aactcattga tttttattta
     3421 cagttaaaaa tcaaccttac aggcattata gcgtactgtt ttcgttttca caattaaaac
     3481 agctgttgta tttagaagaa tttttttcaa aaaattaaaa catattcctt aaaaactaat
     3541 tatttcctac atattaaaat ataatacttc catttccaat aaaaatacat ttataatagg
     3601 aaagataaac agctgaaaag ggagatttca aatgaagaaa aaaacgttca aaaaactatt
     3661 gtttctaggg ggcacagctg ctcttttatt aagcggtaca agttattacc tacatatcca
     3721 ttctccagaa catacaatga acactgcaca aaaaagttca acacaaaaaa cgaaaaagac
     3781 aaaagagaaa gcagaagttt tactagatgt tccattagag agccagtttg attctccttc
     3841 tttagaaaat ggctgtgaag tcacttcgct ttctatgctt ttatcttttt atggttatga
     3901 aacaactaaa aaccagttag ctgagcaatt ggattacgtc cctgttttca atgcagatgg
     3961 gactcatggc gatcctaatg aaggatttgt tggtgatatc agtggcggag actgggctat
     4021 ggtcgtctat gttcccccag ttgcctccct tgctcagcaa atcgtccaaa cggattatca
     4081 cacttttcca aaaaccgatg ctcaactcga tgatatcaaa aaagcactat ctaaaggaaa
     4141 tcctgtctgg acttctgtga ctatcaattt tgaagtccca gatgaaactg attttatgac
     4201 ttggaccaca aataatggag aagtaagagt cactcctctt gttcatgctt gtgtagtcac
     4261 tggatatgac aaagaaaata tttatgtcaa cgatccctat ggtgtaaaaa atcgagcagt
     4321 accgatcaac gatttttcgg cgattttcac tgcaatgggt ggacagatgc taactttgga
     4381 gaaaagctag taattcaaac aaaaatccct tatactcaag ataccaaacc tttgaaggga
     4441 gcgtagataa tggaaaaatt cacacataaa aaaatggatc caaatgaaat tccgattatt
     4501 tttgtccgtg actgcaaggg aaatgttcaa ggtaaagtct ctattaatga gtggaacgag
     4561 cgtcgcagac ctgctaccct caatgaactg gaaatcaaat tgtatcgtca atctcttgtt
     4621 tattacgcag atcaagagta cgagaaagcc accgatttat tgaaatttct aattgctcga
     4681 acagagtata ctcgctttga atatattgaa cgtttagcaa acatctatca tattatgaat
     4741 gaacctgtca aagaatatca gttattggac accgtcttgt cagtagcaga actgattgcc
     4801 cttcctgctg gattagaaaa gaaactagta cgccgccttt tacgagtaaa gcaacaacta
     4861 tccgatcaag aaaaataact tgcgtttttt taggaaagtg ctatgattat tttgttgaat
     4921 aaatgggatg tagccaaact ggtaaggcac ttgactctga atcaagcaat tgtaggttcg
     4981 aaccctgcca tcccaatttg atgtgcttgc tgaaaaaatg aaccccctgt aaaagaacaa
     5041 tctttttgca gggggattat tgtattattt ttactacttg tatttagttt acataattaa
     5101 attatgaata ataaaatcgg gcgtattctt aatacaaaaa ctgatttacc caataattgc
     5161 taatgctttc tccggaactt tttctctagg aacttcctca tacgtttcta tgtgtgctcc
     5221 tttattgcta attttcaaat aatgattttc ttttaacaca gaaagaccat taaattcaac
     5281 cgttcgttct ttcccattgg catcagcagc tttctgcaca taccttgctg taccatattc
     5341 atttacttcc tctggttttt gtgtctttac atagacctct cctttttcaa ctaatggatt
     5401 tagctgatca agtataccag ggatttctcc ataacttcct tcagtataat atttcagtcc
     5461 aaatagcaaa ataccactaa tcgccgccag tccaatcaaa tatttaatca ctttcatctt
     5521 tctacctcct tgttatatat tcagtatatg ggtcagttaa agaaattcca tccgtcccaa
     5581 gttggagatt tttctcctac tttagaagcg agaatcgagt gtttttttag taaaaatgat
     5641 cgttatttga tattattgac gtgtggataa atcagttttt ctcgttttgt ccatgtaaaa
     5701 aatcatgttg atagcaaagg atgtgttcaa aatgaatcaa gaagaattat ttcaaaaagt
     5761 caaggaaatg attaagaacg gcaatttcga tggcgcaaaa cgatttatcg aagaacataa
     5821 agaacaatta ggaccttata aagagaaagc ccaaaatctt ttaaaagatg taaatatc
//
LOCUS       DACDKM010000130         5809 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20834_2016
            SAMN12640370-rid9891663.denovo.130, whole genome shotgun sequence.
ACCESSION   DACDKM010000130 DACDKM010000000
VERSION     DACDKM010000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640370
            Sequence Read Archive: SRR10040891
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5809)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5809)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742835.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742835.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:04:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,012
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 29 of 75
            Pseudo Genes (incomplete)         :: 33 of 75
            Pseudo Genes (internal stop)      :: 19 of 75
            Pseudo Genes (multiple problems)  :: 5 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5809
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640370-rid9891663.denovo.130"
                     /isolate="E20834_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            450..1604
                     /locus_tag="IV334_002512"
     CDS             450..1604
                     /locus_tag="IV334_002512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase C47"
                     /protein_id="HAP8944538.1"
                     /translation="MIILKPKNKIYLGLLFSLMIGIFSIGITNTYASENSNFNINSQP
                     IEEQAINFAKTDFEHSITSILLMENNTETDINYTLGSPFKMLDGENTANSSIFFPVFS
                     DADNTIKYVYTVKKNTNGEYSGSISQFLAKELQNLVDSNITDTTLFFQDNSIYYSDNG
                     QIKLLWKSPLSTEKNGVSSFTEKNDLISVNIKNKIKNFSKKQNFPAVKRVKSVSSGDN
                     FIMINWRIAETQTSFPWCAAYVEAAILCNKTDVRATSARAIMHYTYPNLNNSQIESKS
                     LNQDQIIKYANYCGVYPKKVARILNLSEVQTQLRKGNAIYMGTAGYGNYSNSRHALAL
                     FGWVNQGGYQTYYVWNPWYNYPTVISANAGPVTIPVPGGGFTWDNTITNW"
     gene            1598..2020
                     /locus_tag="IV334_002513"
     CDS             1598..2020
                     /locus_tag="IV334_002513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8944539.1"
                     /translation="MVNKPIVLVMGLVLVLFLTSCSKKQEIQKTELIPFLGTWQTKDH
                     SERLVITNLNTSKNIEQYTILVKENNHKIQIIDKNLNINEQVFIYKATNNLLTLRSNK
                     KQLDYTFSLDSPKKLVFILTTNDGTEGTAKPIEFFKAD"
     gene            3143..4075
                     /locus_tag="IV334_002514"
     CDS             3143..4075
                     /locus_tag="IV334_002514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HAP8944540.1"
                     /translation="MANEIVKYKPELNTIPLRKFTPIEMNLFFSIVSRMRDKGNQKIR
                     FYFDQLKDLSAYKQTAGEVFIKDLTRTYDHLMDLRFGKKSNGGLTIERFTMFTDFEIH
                     GEADNPYVDIKVHEKALPLLNNLESWVRFSLTEFRDLNSSYAKTMFRLLKQYRTQGWA
                     EFSKEDFLELLDIPKSYRQTNINQFVLKPIKEELTPLFKGLTIRKKYGKGRGKPVIGY
                     RFTWKAEINHADDFSKGKQEDLRTKLFNIEHNGELTQEEKWRAKDRILDLPLGTHEAN
                     FKKQQQTEKEEVEKQTLSNELKQDLLGNLQNLFD"
     gene            4092..4607
                     /locus_tag="IV334_002515"
     CDS             4092..4607
                     /locus_tag="IV334_002515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="HAP8944541.1"
                     /translation="MADKTIKDLAEELGVSKTAINKKVSDENRKLWFAKIGNKFVINE
                     IGQKAIKSMFFTENENSNQQPVGEKSKTENHKNANQVSELELFFFLEKQLNQKDIQLQ
                     EKDEQLKQMQKLLDQQQQLTLQANQQIKKLHEQLQLTYAEESSEKEPLSVTEETQKGQ
                     NKEKKWWQFWQ"
     gene            4903..5184
                     /locus_tag="IV334_002516"
     CDS             4903..5184
                     /locus_tag="IV334_002516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8944542.1"
                     /translation="MDILVRNINPKIITRVDKRCQELTTKTGEKWSRNKYLKVLIESD
                     FDRQLMNYKKEQFDLLLEKFIDIQTKNTETLNDYVHTNNQLIETLIGGI"
     gene            5186..5602
                     /locus_tag="IV334_002517"
     CDS             5186..5602
                     /locus_tag="IV334_002517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001232859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8944543.1"
                     /translation="MRKTSVQKHVTLYPSHIDVLDSIIEKNPDINNYSEAIRFLCMSY
                     EPDTNTTTTSNQAKLNAMGKEISIMTEIIAEFADLYLKDTGVLIGTNSKVYQEVKEKI
                     EKQLNPSQRNQPKNRQKETSQTFTSKNFDPTNFLKR"
BASE COUNT         2228 a          961 c          930 g         1690 t
ORIGIN      
        1 aaaaaaaaag actgctcgaa agagcagtct caagcaaaaa ataaacgaac aaacgcaatt
       61 actcacgaac acaaacaaat acaataaaat acctaaataa tgaaaataag tgataaaaaa
      121 caagggtatt tatagtttaa caagcgctca taaaaataac aagtctttaa aaaatgacag
      181 ataaaaatgc acaaagaatc ggacacagat aaccgcgaaa aatcaagctt tagaaagttg
      241 tttttagcta gaccaatgaa aaatcacaaa gtccgcccct gtatccgttg gtagaggggc
      301 tacttggttt agtatacatt atgttaacta gctcgttaaa taacgattat gttcccaaaa
      361 caatatttta tgaactataa caaaaatttg ttatcgcata ttgtatattt taattatagg
      421 tgtatatcac ttaattttaa cacaaaggcg tgattatttt gaaacccaaa aataaaattt
      481 atctaggtct cctcttttcc ttgatgattg gaatttttag tataggaatt acaaatacct
      541 atgcttcaga aaattctaat ttcaatatca actcccaacc aattgaagaa caagcaatta
      601 attttgcgaa aactgatttt gaacactcca taacttcaat tcttttaatg gaaaataaca
      661 ctgaaactga tattaactat actcttggtt cgccatttaa aatgctcgat ggagaaaata
      721 cggcaaatag ttctattttc tttccagtat tttcagacgc tgataatacg attaaatatg
      781 tttataccgt caaaaaaaat acaaatggag aatattcagg aagtatttca caatttttag
      841 caaaagaact tcaaaatcta gtagatagca atataacaga tacaacctta ttttttcaag
      901 ataatagcat ttattattcc gataatggcc aaattaaact actttggaaa tcacctttaa
      961 gtacagaaaa aaatggtgtc tcttccttta cagaaaaaaa tgatcttata tccgtaaata
     1021 taaaaaataa aattaaaaat ttctctaaaa aacaaaattt tcctgcggtt aaacgtgtaa
     1081 aaagtgtctc ctccggagat aactttatta tgattaattg gcgtatagca gaaacccaaa
     1141 caagttttcc gtggtgcgct gcttacgtag aagctgctat cttatgtaat aaaacagatg
     1201 tacgcgctac ttcagctaga gccattatgc attataccta tcctaattta aacaacagtc
     1261 aaattgaatc aaaaagtctt aatcaagatc aaataataaa atatgcaaat tactgtggtg
     1321 tttatcctaa aaaagtagca cgaatactaa acctttcaga ggtccaaaca caactaagaa
     1381 aaggaaatgc catttatatg ggaactgctg gttatggaaa ttatagtaac agccgtcatg
     1441 cacttgcatt atttgggtgg gtcaatcaag gtggttatca aacttactat gtgtggaatc
     1501 cttggtataa ctacccaact gtaataagtg caaatgcagg gccagttaca attcctgtac
     1561 ctggaggtgg ttttacttgg gacaatacca ttacaaattg gtaaataaac ccattgtttt
     1621 agttatgggt ctagtgttag ttcttttctt aactagttgt tcaaaaaaac aagaaataca
     1681 aaagactgag ctgattcctt ttctaggaac ttggcaaact aaagatcact cagaacgtct
     1741 agtaatcact aatttaaata cttcaaaaaa tatagaacaa tataccatct tagtaaaaga
     1801 gaacaatcat aaaattcaaa tcattgataa aaatttaaat ataaatgaac aagttttcat
     1861 ttacaaagca acaaataatt tactaacact gcgctctaat aaaaaacaat tagattacac
     1921 ttttagtttg gattctccaa aaaagctcgt atttattttg acaacgaatg atgggacaga
     1981 agggacagct aaaccaattg aattttttaa agctgattga tgaatagttg tttgatatta
     2041 caaaacaata atttcaccga aggtgaaaca agcgaaactt tacaaaaagt ggctagcatt
     2101 tgctagcctt ttttgtattg cggagcgtga gttttgaggg ggattcaagg gggagtttct
     2161 cccctttgac aagcggttat ttgcaccaaa tacgaaggta tttggctagc aaatatagag
     2221 cttgccaaac cctttagtgt gttttgtaga aagggacctt atagtcactt tcacattaca
     2281 aacgatacgt cctatggacg tatcaaaagg gtgggactta tgagcaaatt aaagcaaaat
     2341 ttggctagca atccttctaa aaaatacacc tattgatcag agtcaaaata aatcagtttt
     2401 cgagtgagcg aatccgaatt tgcaaatctg aagctttgca aatgagtgtg ccggcttccg
     2461 taaaagtcta ggtaacatta gtcgatatat acataaggct ctaatttgcg ttttaaggcg
     2521 ttttaaacgt tctggtatgt aatttatacc aaagcgactt taaaacgcct taaaacgaat
     2581 tttataggtc ttgtaggacg tttcagggtt gttagggctt gccctgaccg cctgtaaggc
     2641 gctttaaaaa caagataagg gattttatct agacttgtct taaaacgccg tagatgagcg
     2701 ctcagagctt cttaaaggac tccttacgaa ctgtttttaa gcaaaggagc ctttaggcga
     2761 ctgcgcagtt caatgtgaga gcgtagcgat cacaccttta aagcgaccgt agggagcaga
     2821 ttcccagttt aggaaatttc gcgaacctgt ttggagaaaa aaagtaggaa atttttaggc
     2881 gggaaatttt gcccccctct tttataatct cttttataac ctctttttaa acctctttac
     2941 cgtcggctgt atccttactc tcccaaggcc tgtagctttt ttagggcatg aaattatctg
     3001 tttataggca tgaaattatc tgtttatagg catgaaatta tctgtttata ggcatgaaat
     3061 tatctgtttt aggtattgtt ttaagacata atatacctta aaattatgtc aataaaatta
     3121 gttagactgg agtatcaaat aaatggcaaa tgaaatcgta aaatataaac cagaattaaa
     3181 taccattcct ttaaggaaat ttactccaat agaaatgaac ttgttttttt ctattgtttc
     3241 tagaatgcgt gataaaggta atcaaaaaat acgcttttat ttcgatcagc ttaaagattt
     3301 aagtgcttat aagcaaactg ctggagaagt ctttataaaa gatttaacaa gaacatacga
     3361 tcatttaatg gatttacgtt ttggaaaaaa aagcaatggt ggattaacca tagaacgatt
     3421 tacaatgttt actgattttg aaattcatgg agaagccgat aacccttatg tggatataaa
     3481 agttcacgaa aaagctttgc ctttacttaa caatttagaa agttgggtac gtttctcttt
     3541 aacagaattt cgagatttaa atagcagtta tgctaaaaca atgtttagat tactaaaaca
     3601 ataccgaaca caaggatggg ctgaattttc caaggaagat tttttagaac tattagatat
     3661 tcctaaaagc tatagacaaa caaatataaa tcagtttgta ctcaaaccca taaaagaaga
     3721 actcacacca ttatttaaag gattaacgat tcgtaaaaaa tatggtaaag gccgaggcaa
     3781 acctgttatt ggttatcgtt ttacttggaa agctgaaatt aatcatgctg acgatttttc
     3841 aaaagggaag caagaagatt tacgtacaaa attatttaat attgaacaca atggagaatt
     3901 aacgcaagaa gaaaaatggc gcgctaaaga tcgaatacta gatcttccgt taggtactca
     3961 tgaagctaat tttaaaaaac aacaacaaac tgaaaaagaa gaagtggaaa aacaaacact
     4021 ttcaaacgaa cttaaacaag atttattagg aaatttacaa aacttattcg attaaagtat
     4081 aaggagtatt tatggcagat aaaacgatca aagaccttgc tgaagaatta ggagttagta
     4141 agacagctat taacaaaaaa gtttctgacg aaaatcgaaa actttggttt gcgaaaatcg
     4201 gaaacaagtt tgtgattaac gaaatcggtc aaaaagctat aaaatcaatg ttttttaccg
     4261 aaaacgaaaa ctcaaatcaa caaccagttg gcgaaaaatc gaaaaccgaa aaccataaaa
     4321 acgccaacca agtttccgaa ttagaactct tttttttcct agaaaaacaa ttaaatcaaa
     4381 aagatataca gctacaagaa aaagacgaac aattaaaaca aatgcaaaaa ttactcgacc
     4441 agcaacaaca actcacctta caagccaatc aacaaatcaa aaaacttcac gaacaactcc
     4501 agctaaccta tgcagaagaa tcttcagaaa aagaacccct ttctgttact gaagaaactc
     4561 aaaaaggaca aaacaaggag aaaaaatggt ggcaattctg gcaataaccc aacaattttt
     4621 attcataaaa attgttgggt tactttcttt ttttgctttt acaagcatag cgacatgatc
     4681 tcacgtcgct attttttaaa gagcataagg aaacgcggta tcactttctt acgaaaggtg
     4741 ctaggtaggc tgtgcctacc acgtttctaa gaaaaaaatt tgctatcact ttttcgccat
     4801 tgtggctagc ttatgtatag tatttcggta aaaaacagct atacaaatac tatatttgta
     4861 tgatatacat aaattataca cacataagaa aggacgtttt ctatggacat tttagttcga
     4921 aatatcaatc caaaaattat tacaagagta gataaacgtt gtcaggaact tacaaccaaa
     4981 acaggagaaa aatggagtcg aaataaatat ttaaaagtgc taatcgaaag tgattttgac
     5041 cgtcaactta tgaattacaa aaaagagcaa ttcgatttat tactcgaaaa atttatcgat
     5101 attcaaacaa aaaatactga aacattgaac gactatgttc atacaaacaa ccaactcatt
     5161 gaaacactta tcggaggaat ataacatgag aaaaacttct gttcaaaaac acgttaccct
     5221 ttatcccagt cacattgacg ttttagattc catcatcgaa aaaaatccag atatcaataa
     5281 ctactcggaa gccattcgct ttttatgtat gagttatgaa cctgatacca atacaacaac
     5341 taccagtaac caagccaaac taaatgcgat gggaaaagaa atttctatca tgaccgaaat
     5401 tatcgcagaa tttgctgatt tgtatttaaa agatacaggc gttcttatcg gtacaaactc
     5461 gaaagtatat caagaagtaa aagaaaaaat tgaaaaacag ctaaatccct cccaacgaaa
     5521 tcaaccaaag aatcgtcaaa aagaaacgag tcaaacattc acttctaaaa atttcgaccc
     5581 aaccaacttt ctaaaaagat aaaaaaataa accaggaaat ttttaatttc ctggtttatt
     5641 ttctagagag cggtcttcca ataagatttg caaatcgatc ccaatttgcg taaaaaaaaa
     5701 gctgctcgta cgagcagcta aacaagaaaa agtcagcttg ttttaagggt aaaataagtg
     5761 taacaaattt tgcgaatgca acgatctttt ttattatacg caaacaatc
//
LOCUS       DACDKM010000131         5791 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20834_2016
            SAMN12640370-rid9891663.denovo.131, whole genome shotgun sequence.
ACCESSION   DACDKM010000131 DACDKM010000000
VERSION     DACDKM010000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640370
            Sequence Read Archive: SRR10040891
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5791)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5791)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742835.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742835.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:04:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,012
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 29 of 75
            Pseudo Genes (incomplete)         :: 33 of 75
            Pseudo Genes (internal stop)      :: 19 of 75
            Pseudo Genes (multiple problems)  :: 5 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5791
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640370-rid9891663.denovo.131"
                     /isolate="E20834_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(99..1229)
                     /locus_tag="IV334_002518"
     CDS             complement(99..1229)
                     /locus_tag="IV334_002518"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HAP8944544.1"
                     /translation="MSKNRPFTVDHVGSFLRPQSIKEARKKVQEGLLSKEELRAIEDQ
                     EIKKLVEDQKAVGIKGITDGEFRRGFWHLDFLEELNGIEGYVPETGYNQTFHGKAAPA
                     YNIRVVDKLSFNPNHPFLKDFAFLKEVVETDDTFVAKATIPSPTMILRQEILSNDGSS
                     KIKEIYPDLSDFYEDLSSTYKAAIHAFYEKGCRYLQFDDTNWAFLADQTKREALLQKG
                     IDPKEMAHICTEIINNALEDKPEDLTITTHICRGNHASSWLFSGGYEPIAEELFSTNY
                     DGFFLEYDSDRAGDFTPLRHWSNKESKVVLGLVTSKFPDLEDTEKIKERIKEAQQFVP
                     LENLSISPQCGFASTEEGNHLTEEEQWKKIQLLQQVAKEVWL"
     gene            complement(1877..3079)
                     /locus_tag="IV334_002519"
     CDS             complement(1877..3079)
                     /locus_tag="IV334_002519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAP8944545.1"
                     /translation="MPKIKNIYQDKKTKKWFFRAYLGIDENGRKVQKTKRGYASQKDA
                     KIAYDKYMETHDFNKTIPNQLCSTNQMTFEEFYKDRFVKWYERQVKSQTYENAQFIFE
                     KRMQFFYHFRVRDITSHDIEDWLFELSQTESRNSRKKVNGETLSKSYINRIRGHLKIV
                     LDRAVKEGVIAKNPVEDVSSLPSENKKVEFWEHSEFQKVMNELKDDKIQTHHRKIVYE
                     MLFYTGIRIGELEALSWSNVDFEKNTITIEKTLIYNTKDDWYFSTPKTKCAYRTIGLG
                     KKLSLKLRQWYDLQKRIGNFEYVAQLDGTFTPPYSFANWLKEAAKKAGVKPIKLHALR
                     HSHVAFLIEQNIQPLSIQERLGHANIQITLGTYGHLYAKSDQQVVSAIDNVQEGAILS
                     KNDNLLPV"
     gene            complement(3139..3342)
                     /locus_tag="IV334_002520"
     CDS             complement(3139..3342)
                     /locus_tag="IV334_002520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 domain-containing protein"
                     /protein_id="HAP8944546.1"
                     /translation="METKCDFMVNRAILIEMGFKPSQAARMIKESKAYLARIEGIDFY
                     NNRQVGVVPSRVIEHLFHIQVAE"
     gene            complement(3484..4716)
                     /locus_tag="IV334_002521"
                     /pseudo
     CDS             complement(3484..4716)
                     /locus_tag="IV334_002521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173388.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
     gene            4879..5691
                     /locus_tag="IV334_002522"
     CDS             4879..5691
                     /locus_tag="IV334_002522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002406512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP8944547.1"
                     /translation="MEIDKQAVGNRIRQIRQELKLSMEKFGKLIGDLPRSTVNNWERG
                     INLPKTETLHQIAEIGHVTNEYLLYGDQENQYILEMLQKKAGKLDPKIEGLIVDEIKH
                     ADLVSEKEMDRMIEFFITNLIPPTEQDQFTFQCIDEKKQLYLGSTNFGKEAQLYLQHD
                     NQNHILHMMPFTFSNFSVDRILVYLANQKSYSYFGKHLPKELGEKAILLYSINQLTSD
                     VRIAPLVYSEETASYQYTEDNQYLLEQQYLYLPFVMEVEKERLLNAAYPSSK"
BASE COUNT         1717 a         1189 c          922 g         1963 t
ORIGIN      
        1 aaataaataa tataaaaggt aaataaaaaa gagtggattt ctccactcca tgaaaagccg
       61 actttttggg acgctttttt ttgccaagcc tcacattttt atagccaaac ttcttttgcg
      121 acttgctgca ataattggat tttcttccat tgttcttctt ctgttaaatg gtttccttct
      181 tctgttgaag caaacccaca ttgaggactg atacttagat tttccaatgg tacaaattgt
      241 tgtgcttctt ttattcgttc ttttattttc tccgtatctt ccaaatctgg aaacttagaa
      301 gtaactaatc ctaaaacaac tttactttct ttgttactcc aatgtcttaa aggagtaaaa
      361 tctcctgctc ggtcagaatc gtattccaaa aagaaaccat cataatttgt tgaaaacaac
      421 tcttctgcaa tgggttcata tccaccggaa aacagccaag aagaagcgtg attcccgcgg
      481 cagatatgtg ttgtgatcgt caaatcttct ggtttgtctt ccagtgcgtt attgataatc
      541 tctgtacaaa tatgggccat ttctttcgga tcaattcctt tttgaagaag tgcttctctt
      601 ttagtttgat cagctagaaa cgcccaattt gtatcatcga attgcaggta acggcaacct
      661 ttttcataaa aagcatgaat cgctgctttg taagtagagg acaagtcctc ataaaaatca
      721 gatagatcag gataaatctc cttgattttt gaagaaccat cattagatag gatctcttgt
      781 cgtaaaatca tcgtgggact agggatcgtt gcttttgcaa caaaagtatc atctgtttcc
      841 actacttctt tcagaaaagc aaaatctttt aaaaatgggt gatttggatt gaaagataat
      901 ttatctacta cccgaatatt ataagctggt gctgcttttc cgtggaatgt ttgattatat
      961 ccagtttctg gaacatagcc ttcgatacca tttagttctt ctaaaaaatc aagatgccaa
     1021 aatccacgcc gaaattcacc atctgtaatt cctttgatgc cgactgcttt ttggtcttcc
     1081 actaattttt ttatttcctg gtcttcgatt gcacgaagtt cttcttttga aagtaagcct
     1141 tcttgaactt tttttcttgc ttctttgatc gattgcgggc gtaagaaact tccgacatga
     1201 tctactgtaa atggtctatt tttgctcatt tcactcactc ttttctttgt tttttgtaaa
     1261 aaaaatcctt ttactagcaa atactagtaa aaggacgatt atactcgtgg taccaccttt
     1321 tttcagaaaa aacattcttt ctctcagcct gacaataaaa tcagtggtct tggtaacgaa
     1381 agactgttcg tttttgccta cctttcgaca aaaaatcctc taaggccatt ttcgacagtc
     1441 aacttctagc tttttacacc aaccaaagcc tctcttgaac cattttctgt ctactttcct
     1501 tctcatcgga tactattaga ataaaattag aatagcatgt ttgaaagaaa agtcaatagg
     1561 gaataagaac aaacaaaaac taatttgtta ttcttagtaa tttgtttgtg gaatacaata
     1621 aagcttttct aatatgataa ttgttcagtc gtcctttcat cttagatcac ttccttaaaa
     1681 tgatcatacc cgtttcaacc acataaaaaa ggacttaatt ttgaaaaatt tcccatcaaa
     1741 actaagtcct ttttattata gttatgcaaa tttgcttatt ttctcgctat ttccgataca
     1801 tcttgaacag gagcaattcg actgaaaaca ctggtgcaaa agcatttatt tgtcttatgg
     1861 tagaaccgtg gaaaactcag actggtaata aattgtcatt ttttgaaaga atagcgccct
     1921 cttgaacatt atcaatcgca cttaccactt gttgatctga ctttgcatat aagtgaccgt
     1981 aagttcctaa agtgatttga atattcgcat ggcccaatcg ttcttgaata gatagcggtt
     2041 gaatattctg ctcaattaga aatgcaacat gagaatgtcg tagcgcgtga agcttaatcg
     2101 gtttgacacc cgctttttta gcagcttctt ttaaccagtt cgcaaaagaa taaggtggcg
     2161 taaatgttcc gtccaactga gcaacatatt caaaatttcc aatacgtttt tgcaagtcat
     2221 accactgtct gagttttaag gaaagcttct tccccaaccc aatcgttcga taagcacatt
     2281 tagttttggg agtcgaaaag taccaatcat cttttgtatt ataaatcaat gtcttctcaa
     2341 tcgttatcgt gtttttctca aaatctacat tcgaccaaga caaagcttca agttctccaa
     2401 tccgaatgcc agtataaaag agcatttcat acacaatctt ccgatggtgg gtttgaatct
     2461 tatcatcctt caattcattc atcacttttt gaaactcact gtgttcccaa aactcaactt
     2521 tcttattctc agaaggaagc gaagaaacat cttcaacggg atttttagca atcaccccct
     2581 ctttaacagc ccgatccaat acaattttta ggtgccctcg aatccgattg atataagatt
     2641 ttgataacgt ttcgccatta acttttttcc gtgaattacg tgactctgtc tggcttagtt
     2701 caaataacca atcctcaata tcgtgacttg taatatctcg aacacgaaaa tgatagaaaa
     2761 actgcatacg cttttcgaaa atgaattgcg cattctcata tgtttgactt ttgacctgtc
     2821 tctcgtacca ttttacaaac ctatccttgt aaaactcttc gaaagtcatt tgattggtag
     2881 aacacagttg attaggaatt gtcttgttaa aatcatgtgt ttccatgtac ttgtcataag
     2941 caattttggc atctttttgt gaagcataac ctcgtttagt cttctgaacc tttcttccat
     3001 tttcatctat acctagatac gctcggaaga accatttttt tgtcttttta tcttggtaaa
     3061 tatttttaat ttttggcatc gttccattcc tccctgtact tgagaaaagc tactttaata
     3121 gtaccgcgct tgtactgttt attccgcaac ttgaatgtga aaaagatgtt caattactcg
     3181 ggatggcacc acaccaactt ggcgattatt ataaaaatca ataccttcaa tccttgcaag
     3241 atatgccttg ctctctttaa tcattcgtgc agcttgacta ggtttaaagc ccatctctat
     3301 caaaattgca cgatttacca tgaaatcaca ttttgtttcc attctctcac ctcctgattc
     3361 tttactcctt ttaaaataat cctctttata atctattgtt tctcattcga tggttgtgtt
     3421 atactcgtct tgtcgaggcg gagtacgttg cacaacttgt tgtgtaagca gtactttgct
     3481 tttttagtag cttgttttct catttgatat actttgtaat tttcgttcat aatactcaac
     3541 ctcctcctga taatactccg ggtctgcctt catattttgg agaagtttga tctgtcttgg
     3601 attcatttct ccttgttcaa taatcaaagg caacaaggaa gaatgaaacg tttgatcgac
     3661 ctgtgcaata aaaactaatg atggtgaaac accatgaatt aaccaactca tagttttctc
     3721 aaaactttga ggaattgtat ctaaagaaat ggtgattgcg ccataattgc taataaaggc
     3781 tgcccatttg gcatcaactt ttgcacctag atcatttgga tcacggtcat aaaaacaaat
     3841 tgctccatta agtaactcaa agaacagttt ggttaaatca tgggtttttg aaataatctt
     3901 tgccaagctt tgcgctttct cttttcgata ccgtagttca aagcgattct ttacttttgc
     3961 ctcttctaaa ggaattcctc gtcgcttccg ttgttcatgg tccttctgat aaaagcaaaa
     4021 acgacactga ctcttctttg agcctaaata caaggtctca ccatttgatt cattgtttat
     4081 gatatccgta ccgccatgag attcactcac ttgaaaagtg gtccacacat ggcctagtat
     4141 taccttatgc ttaagttctg gaatgctgag actccccaca aaatcatcta aagtaaaatc
     4201 aatccgtgta aagttcccct gataatctaa taccttacga aaaaactgtt gccaatttgt
     4261 ttttcgtgtt tttagccatc ttgctaagtg tttacagcct tgacctgaaa actccatcac
     4321 agttccttta accgcatcgt caatggaata ccgaatcgtg attacttgat tccacacgta
     4381 ttggccaata tagccctttg gcgcataatc cagttcctca aaacgttcaa tgggcatttc
     4441 aataatctct tcaattagct ttaaatgttg attcgagaaa aagtgaatgg ttagataatc
     4501 aatcaaaaca cggttaactg gaacgacctc ttcttgcaaa ctatccaaca caagattcaa
     4561 tttttcttca cttaattgtc tcgttccttt ttctactttc gataataact atctcgaaat
     4621 ccccactttt tctgctaaat atcgttgtgt ccaccccctt tcttctcgag aaactcgaat
     4681 ggcttcaccg agatcatgtt gattaacttg gttcatcgac aaacctcctt ctgctattta
     4741 cttgtcaaag aacctattga acttatttta agtacatttt tattgtacgc ctgtatttat
     4801 tttaagtcaa tatgttttga ctttttttaa gtacacacgt atactaaatt taagaactta
     4861 tcctgaggag gagttttcat ggagatagac aagcaagcag tcggcaatcg aattcggcag
     4921 attcgccaag aattaaagtt gagtatggag aaatttggca aactaattgg tgatttaccg
     4981 cgaagtacag tcaataactg ggaacgtggg atcaaccttc caaaaaccga aacccttcac
     5041 caaatcgcag agataggtca tgtcaccaat gaatatcttt tgtatggtga tcaagagaat
     5101 caatacattt tagaaatgct ccaaaaaaag gcaggtaagc ttgatcctaa gattgaaggt
     5161 ctcatagtag acgaaatcaa acacgctgac cttgttagtg aaaaagaaat ggatcgaatg
     5221 attgaatttt ttattacaaa tctcattcca ccaactgaac aagaccagtt tactttccaa
     5281 tgtatcgatg aaaaaaagca gctttatctt ggttcaacta attttggtaa ggaagcccag
     5341 ctctatttgc aacatgataa tcaaaatcat attctgcaca tgatgccctt taccttctcc
     5401 aactttagtg tggatcgcat actcgtctat ttagcaaatc aaaaatccta ttcctatttt
     5461 gggaaacatt tacccaagga actaggtgag aaagccatcc ttctatactc aattaatcaa
     5521 ttgaccagcg atgtgcgaat tgccccgttg gtctattcag aagaaaccgc ttcttatcag
     5581 tacacagagg acaatcaata tctactagaa cagcaatatc tttatctacc attcgtgatg
     5641 gaagtcgaga aagaacgcct gttaaatgca gcgtatccat catcaaaatg aggtgacaaa
     5701 attgataagc acgtgacaaa gaaaatactg ctaggacact ccccaatggg tagttgtcac
     5761 agtttcctgt tctttgttag ccccctgtta g
//
LOCUS       DACDKM010000132         5778 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20834_2016
            SAMN12640370-rid9891663.denovo.132, whole genome shotgun sequence.
ACCESSION   DACDKM010000132 DACDKM010000000
VERSION     DACDKM010000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640370
            Sequence Read Archive: SRR10040891
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5778)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5778)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742835.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742835.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:04:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,012
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 29 of 75
            Pseudo Genes (incomplete)         :: 33 of 75
            Pseudo Genes (internal stop)      :: 19 of 75
            Pseudo Genes (multiple problems)  :: 5 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5778
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640370-rid9891663.denovo.132"
                     /isolate="E20834_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            91..672
                     /locus_tag="IV334_002523"
     CDS             91..672
                     /locus_tag="IV334_002523"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HAP8944548.1"
                     /translation="MKELVERIQKDGRILGEGVLKVDSFVTHQVDPVLMEQMGKRFAE
                     VFKEQGITKVVTIEASGIAPALFAARELDVPMIFARKAKSLTMDEELLTASVYSFTKQ
                     VTSTISISRKFLSSEDKVLIIDDFLANGQAAKGLIELCQQAGAQVEGIGIVIEKSFQD
                     GRQLLEEMGLRVVSLARIASLTNGQVEFLEEDA"
     gene            675..1982
                     /locus_tag="IV334_002524"
     CDS             675..1982
                     /locus_tag="IV334_002524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine permease"
                     /protein_id="HAP8944549.1"
                     /translation="MEKETQNGKAAVLGLQHLLAMYAGAVAVPLLIGTGLGFNQEQMT
                     YLISIDIFMCGLATLLQLTVNRFFGIGLPVVLGCAIQAVAPLILIGTNEGVGAIYGSI
                     IASGIFVVLVSGVFSKIKKLFPPIVTGTVITVIGLTLIPVAIEKMGGGNATAADFGDK
                     TNLLLAFVTILLIVGVQMLAKGFIRSIAVLIGLVGGSILAAFMGMIDLSAIGDAPLFH
                     VPQPFYFGKPTFDVWSILLMIIISMVSMVESTGVYFALGDITGKNVGEEELKKGYRAE
                     GLAVILGGIFNTFPYTGFSQNVGLVQLSGIKTRKPIYFSAFFLIVLGLFPKIGALAQI
                     IPEPVLGGGMLVMFGMVAVQGIRMLLEVDFSNDKNLLIVAVSVGFGLGFNVTPTLFSQ
                     MPQTLQMFTGNGIVMSSITAIILNLIFNGLKKDEEILEEAFKK"
     gene            2275..2763
                     /gene="purE"
                     /locus_tag="IV334_002525"
     CDS             2275..2763
                     /gene="purE"
                     /locus_tag="IV334_002525"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HAP8944550.1"
                     /translation="MTAKISVIMGSTSDWETMKEACQILDEFGVSYEKKVVSAHRTPD
                     LMFEYAQNARKKGIKIIIAGAGGAAHLPGMVASKTTLPVIGVPVQSRTLNGLDSLLSI
                     VQMPGGVPVATTAIGKAGAINAGLLAIQMLSMYDLELEAKLAERRSMLAKTVIESSDQ
                     LG"
     gene            2747..3880
                     /gene="purK"
                     /locus_tag="IV334_002526"
     CDS             2747..3880
                     /gene="purK"
                     /locus_tag="IV334_002526"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HAP8944551.1"
                     /translation="MINLVKPLLPGSTIGIVGGGQLGRMMAISAKEMGFKVGVLDPVS
                     DCPAAQVADWHIESVYDDTFALKELARRTDVITYEFENVSVEALNAILPMSFIPQGTD
                     LLAITQDRLLEKSFLETNNIVIAPYATIVSPTDIQDAIDGIGYPCVLKTTRGGYDGKG
                     QYVLKSRADLAPAMDLLREGTCVLEAWIPFEKEISIMVAGNGQGDFTTFPVVENIHHN
                     NILHETIAPAAIDQDVIEEAERIARVIAEAVSLSGVLGVEMFLTKTGGLYVNELAPRP
                     HNSGHYSIEACSMSQFDAHIRGICGWPLGEKVRLISNAVMVNVLGEQLLESYRLIENK
                     PDWQFHFYGKKQAKKGRKMGHITILTEDIQQTLEEIKQTNIWD"
     gene            3893..5188
                     /gene="purB"
                     /locus_tag="IV334_002527"
     CDS             3893..5188
                     /gene="purB"
                     /locus_tag="IV334_002527"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HAP8944552.1"
                     /translation="MLERYTRPEMGNIWTEENKYRAWLEVEILADEAWAELGEIPQED
                     VKKIREHASFDIDRILEIEKLTRHDVVAFTRAVSETLGEERKWVHYGLTSTDVVDTAY
                     GYLIKQANDILREDLKRFTEIIGEKAKEHKYTVMMGRTHGVHAEPTTFGLKLATWYSE
                     MKRNIERFEHAAKGVEAGKISGAVGTFANIPPFIEEYVCKQLGIRPQEISTQVLPRDL
                     HAEYFSAMALIATSIERFATEIRGLQKSETREVEEFFAKGQKGSSAMPHKRNPIGSEN
                     MAGLARVVRGHMVTAYENVTLWHERDISHSSAERIILPDTTILLNYMLNRFGNIVKNL
                     TVFPENMKRNMSATFGLIYSQRVMLKLIDKGMTREEAYDLVQPKTAYAWDNQVQFRPL
                     LEEDPTITSVLSKEEIDDAFDYHHHLKNVDVIFKRVGLE"
     gene            complement(5368..5745)
                     /locus_tag="IV334_002528"
     CDS             complement(5368..5745)
                     /locus_tag="IV334_002528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8944553.1"
                     /translation="MEDAIKQGQLFLSELTAEERSCLYNDLLSIKDVYLCNKDTSFYT
                     FRKIRRATKRCAECRKRTFLYQTEHKGELSISQIHEQKRFKNHYRIQILFNGQEMEYA
                     FLENLLQHLHNQHFSYNSQAIYA"
BASE COUNT         1789 a          995 c         1332 g         1662 t
ORIGIN      
        1 tggagggtgt ttttctttaa cagaaacata gtaggttttt gcacaaaagc ggttatttcg
       61 gtattaataa atgataaagg agcaaataaa gtgaaagaat tagttgaacg tattcaaaaa
      121 gacggacgta tattaggcga gggcgtatta aaagtcgaca gcttcgtcac tcaccaagta
      181 gaccctgtat tgatggaaca aatgggaaaa cggtttgcag aagtatttaa agaacaagga
      241 atcacgaaag ttgtgaccat tgaagcttct gggatcgcac cagctttatt tgctgcaaga
      301 gaattagatg ttccaatgat cttcgctaga aaagcaaaaa gtttgacgat ggatgaagaa
      361 ttattgacag cttctgttta ttcttttacg aaacaagtga caagtacgat ttctatttct
      421 cgtaaatttt tatcttcaga agacaaagta ttgattatcg acgatttctt ggcaaacgga
      481 caagcggcaa aaggattgat cgaattatgc cagcaagctg gagcgcaagt agaaggaatc
      541 gggatcgtca tcgaaaagtc tttccaagat ggacgtcagt tacttgaaga aatgggattg
      601 agagtcgttt ctcttgcgcg tattgcttcc ttgacgaatg gacaagttga gtttcttgag
      661 gaggacgcat aatcgtggaa aaagaaacac aaaatggaaa agctgcggtt ttagggctgc
      721 agcatctgct agcgatgtat gcaggagcag tagcggtgcc tcttttgatc ggtacaggac
      781 taggctttaa tcaagaacag atgacttatt tgatttcaat tgatatcttt atgtgtggat
      841 tagcgacatt attgcaattg acagttaacc gatttttcgg aattggcttg cctgtcgttt
      901 taggatgtgc gatccaagca gtagctccac tgatcttgat cggaaccaat gaaggagtag
      961 gagctatcta cggatctatt atcgcttctg ggatctttgt tgtgttagtt tctggtgtct
     1021 tttctaaaat taaaaaacta ttccctccta tagtaacagg aacggttatc accgttattg
     1081 gtttgacttt gattcctgta gcaatcgaga aaatgggtgg aggtaatgca acagcagctg
     1141 attttggtga caaaacaaat ctgctacttg catttgtgac gattctccta attgtaggcg
     1201 tgcaaatgct ggcaaaaggg ttcattcgtt caatcgcggt attgattggt ttagtcggag
     1261 gaagcatctt agctgctttc atgggaatga ttgatctgag cgcgattgga gatgcgccgt
     1321 tgttccatgt tccacaaccc ttttattttg gtaaaccaac tttcgacgta tggtcgattt
     1381 tactaatgat catcatttcc atggtcagca tggtagaatc aacaggtgta tattttgcac
     1441 ttggtgacat tactgggaaa aatgttggag aagaagaatt gaaaaaggga tatcgtgcag
     1501 aaggacttgc cgttatctta ggcggtattt tcaatacatt cccttatact ggcttttctc
     1561 aaaatgtcgg attggttcaa ctttcgggaa tcaaaacaag aaaacccatc tatttctctg
     1621 cattcttctt gattgtttta ggattattcc ctaaaatcgg tgcgttagca caaatcatcc
     1681 ctgagccggt acttggtgga ggaatgttag tcatgttcgg gatggtggca gtccaaggaa
     1741 tccgtatgct gttggaagtt gatttcagca atgataaaaa tctattgatt gttgctgttt
     1801 ctgttggatt tgggctgggt ttcaatgtta cgccgacatt attcagtcaa atgccgcaaa
     1861 cattgcaaat gttcactgga aatggcatcg taatgagtag cattacggct atcatattga
     1921 atttgatctt taatggattg aaaaaagatg aagaaatatt ggaagaagcc ttcaagaagt
     1981 aatagagcaa aagaaaggtt gtgaaatcag cgttttgacc tgattgttag agaaacaaac
     2041 ggaaaaatag ttcctcgtat tttgccgttt gtttgagcta ataccgaggg tcagctgatt
     2101 gaacaccgtt tatccataaa aaggaatgtg acctaacttt tgtcacattc ctttttttca
     2161 tctttaataa acacgaacaa taaaatgttt ttctttgtta acattcgaaa tttctttgac
     2221 atcaaaaaaa gtgattgtta aaatatgaat gtagaaaaac tgggaggaaa aacaatgaca
     2281 gcaaaaatct ctgtgattat ggggagtact tcagattggg aaacaatgaa agaagcatgt
     2341 caaatattgg atgaattcgg tgtgtcttat gaaaaaaagg tcgtttctgc tcatcgaacc
     2401 ccagatttga tgtttgaata tgcacaaaac gctcgaaaaa aaggaatcaa aatcattatt
     2461 gcaggagcag ggggagccgc gcatctgccg ggtatggtcg catcaaaaac aactttgccg
     2521 gttatcggtg taccggtcca atcacgaaca ttaaatggtt tggattcgct tctttcgatt
     2581 gtccaaatgc ctggaggagt acctgtagct actacagcaa tcggaaaagc gggagcaatt
     2641 aatgctggac tacttgcgat acagatgctt tccatgtatg atttagaatt ggaggctaaa
     2701 ttggctgaaa gacgttcaat gttggctaaa actgttatag aaagtagtga tcaacttggt
     2761 taaacctttg ttgcctggtt caacgatcgg gatcgtcggt ggcggtcaat taggtcgtat
     2821 gatggcgatt agtgcaaaag aaatgggctt taaagtaggt gtgctggatc ctgtgagcga
     2881 ttgtccagcc gcacaagttg cagattggca cattgaaagt gtctatgatg acacctttgc
     2941 gctgaaagag cttgccagaa gaacagatgt cattacctat gaattcgaaa acgtcagtgt
     3001 agaggcgtta aatgcgattt tacccatgtc atttattcct caagggacag acttgcttgc
     3061 gattacgcaa gaccgcctgt tagaaaaatc ttttttggaa acgaataaca tcgtgattgc
     3121 tccttatgca acgatcgtca gtccgacgga tatccaagat gcgattgacg ggatcggcta
     3181 tccttgtgtt ttgaaaacca ctcgaggcgg ctatgatggc aaggggcagt atgttttgaa
     3241 aagtcgggcg gatctagctc ctgccatgga ccttttaaga gaaggaacct gtgttttaga
     3301 agcatggatc ccgtttgaaa aagagatttc gatcatggtg gcaggaaatg gtcaaggaga
     3361 tttcacgact tttccggtcg tggaaaacat ccaccacaac aatattcttc atgaaacgat
     3421 tgctccagcc gcgattgatc aggatgtgat cgaggaggct gagcgtattg cccgtgtgat
     3481 tgcagaagca gtgagccttt caggtgtttt aggggtagaa atgtttttaa caaaaacagg
     3541 cgggctatat gtgaatgaat tagctcctcg tcctcacaat tctggacatt attctattga
     3601 agcttgttct atgagtcagt tcgatgccca tattcgaggt atttgtggtt ggccattagg
     3661 agaaaaagta cgtttgataa gcaatgcagt aatggtcaat gttttgggag aacaactgct
     3721 ggaaagctat cgcttgattg agaataagcc agactggcaa ttccatttct atggaaaaaa
     3781 acaagcaaaa aaaggtcgca agatgggaca tatcactatc ttgacagaag acatccagca
     3841 aacgctggaa gaaatcaaac aaacgaatat ttgggattag aggagaaaaa acatgttaga
     3901 acgttataca cgccctgaaa tggggaatat ctggacggaa gagaacaaat accgtgcgtg
     3961 gctcgaagtc gagattttag cagatgaagc ttgggcagaa cttggtgaaa tcccgcaaga
     4021 agatgtgaaa aaaattcgtg agcatgcatc atttgatatc gatcggatct tggaaatcga
     4081 aaaattaaca cgccatgatg tcgttgcgtt tacacgagcg gtttctgaaa cattgggaga
     4141 agaaagaaaa tgggtccatt atggtctaac aagtacggat gtcgtagata ctgcttatgg
     4201 ctatctcatc aagcaagcaa atgatatttt acgggaagac ttgaaacgtt ttacggaaat
     4261 cattggcgaa aaagcaaaag aacacaaata tacagtgatg atgggtcgga cacacggtgt
     4321 ccatgctgaa ccaactactt ttggattgaa acttgccaca tggtattcag aaatgaaacg
     4381 gaacatcgaa cgatttgaac atgcggcaaa aggagtagaa gcaggaaaaa tctcaggagc
     4441 agtgggaacg ttcgcaaata tcccgccatt tattgaagag tatgtatgta aacaattggg
     4501 gatccgtcca caagaaatct ctactcaagt tctccctcgg gatctacatg ctgaatattt
     4561 ctcagcgatg gcgcttattg ctacaagtat tgaacgtttt gcaacagaga tacgcggatt
     4621 gcaaaaatcc gaaacacgtg aagtagaaga attttttgcg aaaggtcaaa aaggttcttc
     4681 tgctatgccg cacaaaagga atccgatcgg ttcagaaaat atggctggtc tagccagagt
     4741 ggtgcgcgga cacatggtca ctgcctatga aaatgtcaca ttatggcatg aaagagatat
     4801 ttctcattct tcagcagaac gaatcatctt gcctgatacc acgattttat tgaattacat
     4861 gttaaatcgt tttggcaata tcgttaaaaa tctaacggtc tttcctgaaa acatgaagcg
     4921 gaatatgtct gcaacatttg gtttgattta tagccagcgc gttatgctga aattgatcga
     4981 taagggaatg acaagagaag aagcttatga tcttgttcag ccaaaaactg cgtacgcttg
     5041 ggacaaccaa gtgcagttcc gcccgctgtt agaagaagat ccaaccatca catcggtatt
     5101 atctaaagaa gaaatagacg atgcctttga ttatcatcat catttgaaaa acgttgacgt
     5161 gatattcaaa cgagtaggtt tggaataaag aagagtgagt tgcaaaattc aattaaaata
     5221 gaatagcaaa aataaccaaa aaagaagcag atccgttagc ggttctgctt cttttagttt
     5281 attatatttt cttatcaata ataggaacct gattttaaaa gcggctacct ctttaccgtg
     5341 cctctttaca tacaaaaaat agaggaatta agcgtaaatg gcttggctat tataactaaa
     5401 atgctggtta tggagatgtt gcagcaagtt ttcgagaaaa gcgtattcca tctcttgtcc
     5461 attgaataag atctggatac gataatgatt tttgaaacgc ttctgttcat gaatctgact
     5521 gattgaaagt tcgcctttgt gttctgtctg ataaagaaaa gtccttttac ggcattcagc
     5581 acagcgcttg gttgctcggc ggatttttcg gaatgtataa aaactagtat ctttattgca
     5641 taaataaacg tctttgatcg acagcaaatc attatataga caggaacgtt cttcagctgt
     5701 taattctgat aaaaaaagct gaccttgttt tattgcatct tccatcggca agtgcctcct
     5761 cgaacccatt atccaaat
//
LOCUS       DACDKM010000133         5715 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20834_2016
            SAMN12640370-rid9891663.denovo.133, whole genome shotgun sequence.
ACCESSION   DACDKM010000133 DACDKM010000000
VERSION     DACDKM010000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640370
            Sequence Read Archive: SRR10040891
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5715)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5715)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742835.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742835.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:04:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,012
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 29 of 75
            Pseudo Genes (incomplete)         :: 33 of 75
            Pseudo Genes (internal stop)      :: 19 of 75
            Pseudo Genes (multiple problems)  :: 5 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5715
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640370-rid9891663.denovo.133"
                     /isolate="E20834_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(198..980)
                     /locus_tag="IV334_002529"
     CDS             complement(198..980)
                     /locus_tag="IV334_002529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8944554.1"
                     /translation="MENSSNSAEVNVSTSTSEVVKCSQETTSLPEELLGDWQINNKDA
                     GIEISDSKAVFRIGRSQVESKDYYVDVNNKMLLHVYFSFEYDSVKYIEVSIERTGYSE
                     FISKSVGILTMHTKDGQYISLGVKRVSGENNETEAVTEQTMDERIDSSKSNETEVDTG
                     KTIDERIDSFIETYTAIPPTWKLVNEDKGITINTDTEISTDAQSLSDLLANLTGISNA
                     VTEQVGKDIPLRLLENGKKDYSVVFKNGQVTDYNEVFKKVIE"
     gene            complement(1045..1881)
                     /locus_tag="IV334_002530"
     CDS             complement(1045..1881)
                     /locus_tag="IV334_002530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAP8944555.1"
                     /translation="MRVIKTLNNSLILCLDKQGKEVILMGKGIGFQLKKGDLVPADKI
                     QKVFKLDSLSDALKKDYIKLFEAAPDDLIICVKRIIDYANAKFDFKLNKSLFFTLVDH
                     LKHAIERSQKGIVFQNRLLIEIQKYYPKEFEIGCYAIQLINKKMNVQLPEEEAGNIAF
                     HIVNAQSEQANMEETMLSVRALKDILAMLGYLFPEKKVDKDSMLYLRFVTHLQFFIAR
                     MIKKEELPEKDDFLLASVKQRFPKEYRAALKIKEYVQEEMGASANQDELLYLTLHISR
                     VY"
     gene            complement(1899..3308)
                     /locus_tag="IV334_002531"
     CDS             complement(1899..3308)
                     /locus_tag="IV334_002531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010757186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HAP8944556.1"
                     /translation="MQYKQLSAFPEKFLWGASISAYQAEGAYQEDGKQPSIIDKYQHT
                     EGVANFEVASDFYHHYKEDIQLFKELGLKAFRFSIAWTRIMDIENNKTNQKGISFYHQ
                     VIDECLNQGIEPIVTMYHFDLPYFLEEQGGWLNRATIDYFVDYVQVLLTEYGDKVNYW
                     LTINEQNTMILHPGAIGLPPSGELPSKKELFQINHHVLLAQARVIHLYHQLNLKGKIG
                     PAINLTAMYQATSSPEDAIAAHNWETLRGWSFLDVAVRGKYNYLFENYLNDRGLYPKI
                     EAEDQAILSSGKPDFIAINYYSTATIAASKNDGSDVSARAGDQQIMLGEEGVYRAAEN
                     PYVDKTPYGWVVDPTGLRLTLRKLYDRYDLPILITENGYGAPDIVEEDRRINDQDRID
                     YLAKHILAIQAALTDGVDVFGYLPWSAIDVVSTHQGFNKRYGFIYVDRTDENLKELKR
                     IKKESFFWYQKVIARNGLA"
     gene            complement(3322..5145)
                     /locus_tag="IV334_002532"
     CDS             complement(3322..5145)
                     /locus_tag="IV334_002532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP8944557.1"
                     /translation="MDYREVGRNVLQQVGGKENVVSLTHCATRLRFELKDKRKADTKA
                     LEKTLGVISVVDSGGQYQVVIGNEVQTAFKEIQKEVGTSIKKGPSSDNQESIISRLIS
                     VISTTFTPMIPAITGAGMVKAILAVLTLTGVLSDSSQTYIILNTIADAAFYFMPVLLA
                     YGAAIKFDVNPILAITVAGILLHPNISQLFSTGDAVSFLGIPVLAADYAGSVLPIIFT
                     VWIMSYVEPFADRISPSFIKFFTKPMLVFLIMGPLALVVIGPFGTLLNDLIAMGAEWI
                     NGKAAWLIPFLMGGLQPFLVVTGTAWAMTPIATMQLSSTGSEMINGPGMLASNIAQSG
                     ATFAVAAKTKNKELKQLAVSTGITALMGITEPSLYGVTLKLKKPLVASMIGGAIAGLY
                     AGLSGLVRYAFVSPGLAALPAFIGENPMNIVHAIITCVIAFVATFIIAYLIGFDDTSD
                     ESEQTAIAEAVIVENPVKGTVCPISEVNDAVFSKGLLGKGVAIHPEENSIYSPVEGEV
                     ITILESKHAIGLRSKEGIELLIHVGIDTVNLKGEPFEVFVKAGDQVSAGQLLINADFA
                     AIRNAGYDDTVIVVVTNSENYAGISEASGSLKEHEPLMQIS"
BASE COUNT         1745 a         1248 c          943 g         1779 t
ORIGIN      
        1 ctgaaacagt ctaaaacact tatatatcaa cgtttaatga aactatctcc gtttcatttc
       61 agatacaaat aaatatagtc gggctccaaa tccgaaaaaa aataccccca gtagcttatc
      121 ctaagctact ggagatgtat aagtacggtc atataacaac ggggtagggt aaataaatga
      181 aaaagatgga agaatcatta ttcaataact tttttaaata cttcgttgta atcagttacc
      241 tgtccgttct taaataccac agaataatct tttttcccat tttcaagtaa ccgaagtgga
      301 atgtctttac caacttgctc tgtcactgca ttactgatac ctgttagatt agctaataaa
      361 tctgataaag attgagcatc tgtagaaatc tctgtatcag tatttatcgt gattccttta
      421 tcttcattta caagcttcca agtaggtggt atagctgtgt atgtttcgat gaatgaatca
      481 attctttcat ctatcgtctt cccagtatct acttctgtct cattactctt agatgaatcg
      541 attctttcat ccatcgtctg ctccgtaact gcttctgtct cattattctc accggaaact
      601 ctcttcacac ccaaacttat atattgtcca tctttagtgt gcatagttaa aattcccacg
      661 cttttactaa taaattcact ataaccagtt ctttcaatag agacttcaat gtatttcaca
      721 ctatcatact caaaagaaaa atagacatgt aaaagcattt tgttgtttac atctacataa
      781 taatcctttg actctacttg agaccttcct atacggaata cagctttact gtctgatatc
      841 tctataccag catccttatt attaatctgc caatctccaa gtagctcttc tggcaagcta
      901 gtagtttctt gtgaacactt taccacttca ctggtagagg tcgaaacatt cacctccgca
      961 ctgttagaag aattttccaa ggaatcagga gccttagcac aaccaacaag tctttatata
     1021 ttgtgcagcc gatattttct tcattcagta cacccttgaa atatgcagtg ttagataaag
     1081 cagttcgtcc tgatttgctg aggcacccat ctcttcttga acgtactcct tgattttcaa
     1141 ggccgcccga tactctttcg gaaaacgctg cttcactgaa gccagcaaaa aatcatcctt
     1201 ctccggcaac tcttccttct taatcattcg ggcaataaaa aactgtaaat gcgtcacaaa
     1261 tcttaagtaa agcatcgaat ctttatcaac ctttttttcg ggaaacagat atcctagcat
     1321 agccaagata tcctttagtg ctcttaccga gagcatggtt tcttccatat ttgcttgttc
     1381 actttgggca tttacaatgt gaaacgcaat gtttccagcc tcctcttctg gcaactgaac
     1441 attcattttt ttgttgatca attggatagc atagcagccg atctcaaatt cttttgggta
     1501 atacttttga atttcaatca gcaggcgatt ttgaaaaaca atgccttttt gactgcgttc
     1561 aatggcatgc ttcaagtgat caaccaaggt aaaaaataga cttttattca gcttaaagtc
     1621 aaacttggcg ttggcataat cgataattcg cttgacgcaa ataattagat catccggagc
     1681 agcctcaaac agtttaatgt aatccttttt tagagcatca gaaagtgaat ctaatttaaa
     1741 tactttttgg attttgtcag ccggaaccaa atcgcctttc tttagttgaa atccaatccc
     1801 tttccccatc aaaattactt ctttgccttg cttatctaag caaagaatta aactgttgtt
     1861 taacgtttta attactctca ttagattcac cgttctctct aagctaagcc atttcttgcg
     1921 attacttttt gataccaaaa gaagctttct ttttttattc gttttaattc tttaagattc
     1981 tcatcggttc ggtcaacata tataaaacca taacgcttat tgaagccttg atgggtacta
     2041 acaacatcaa tcgctgacca aggtaaataa ccaaaaacat ctacaccatc tgttaaagct
     2101 gcttgaatag ccagaatatg tttggctaga taatcaattc gatcctggtc attaattcgt
     2161 ctatcttcct caactatgtc gggtgcccca tagccatttt cagtaattaa aataggtaaa
     2221 tcatagcgat cgtacaattt cctcaatgtt aatcgtagtc cagtagggtc aactacccag
     2281 ccatatggcg ttttatctac ataaggattt tcagctgccc ggtagacacc ttcttctccc
     2341 aacatgatct gttgatcccc cgctctagcg gaaacatctg atccgtcatt tttgctggca
     2401 gcgatggtgg cagttgaata ataattaatc gcaataaaat cgggtttacc ggaggataaa
     2461 attgcctgat cctctgcctc aattttagga tataatccac gatcattcaa atagttttca
     2521 aacaaataat tgtattttcc ccgaactgct acatctaaga aagaccagcc tctcaatgtt
     2581 tcccaattat gggctgcaat tgcatcttct ggactggagg tagcttgata catagccgtt
     2641 aaattgattg ctggaccgat ttttccttta agattcaact gatgatacaa gtgaattact
     2701 ctagcttgag ctaataaaac atggtgatta atttgaaaga gttctttttt tgaaggcagc
     2761 tctccacttg gtggcaaacc aatcgctcca ggatgaagga tcatcgtgtt ttgctcatta
     2821 attgtcagcc aatagttcac cttgtcacca tactcagtaa gcaatacctg tacatagtcc
     2881 acaaaataat cgatagttgc tcgatttaac caaccgccct gctcctctag aaaataaggt
     2941 aaatcgaaat ggtacatggt gacaatcggt tcgattccct gattcaggca ttcatcaatt
     3001 acttgatgat aaaaagaaat tcccttttga ttggttttat tattttcaat atccataatt
     3061 cgcgtccaag cgatggaaaa gcgaaaagct tttaagccta attctttaaa cagctgtata
     3121 tcttctttat agtgatggta aaaatccgag gcaacttcaa aattagctac cccttctgta
     3181 tgctgatact tgtcaataat agacggttgt ttcccatcct cctgataggc accctctgcc
     3241 tggtaggcac taatggaggc accccataaa aacttctctg gaaatgctga aagctgtttg
     3301 tattgcacaa aaatcctctc cttaactaat ttgcatcaat ggttcgtgtt cctttaagct
     3361 tccgcttgct tcactaattc ctgcgtaatt ttcactgttg gtcactacca caatcactgt
     3421 atcatcgtag ccagcgtttc taatggcagc gaaatcggcg ttgattaata attgtcctgc
     3481 tgacacctga tctcctgctt ttacaaatac ttcaaagggt tcccctttta aattcacggt
     3541 gtcgatcccc acatggatca ataattcaat accttcttta gaacgtagac caatcgcatg
     3601 tttagattct aaaatagtaa tgacttctcc ctcaacaggt gaataaatgc tgttttcttc
     3661 tggatgaatg gctacgccct ttcccaatag tcccttagaa aagacagcat cgttcacttc
     3721 agaaatagga cacacggttc cttttaccgg attttcaaca atgactgctt cggctattgc
     3781 cgtttgctct gattcatcag aagtatcgtc aaatccaatt agataagcaa tgataaaggt
     3841 tgcaacaaag gcgattacac acgtaataat tgcgtgaaca atattcattg gattttctcc
     3901 aataaaggct ggtaaggctg ctaagccagg ggaaacaaag gcatatctga ctaaaccgct
     3961 caagccagca tacaaaccag caattgcacc accaatcatg gaagcaacta atggtttttt
     4021 cagcttcaag gttactccat agagtgaagg ctcagtaatt cccattaaag cagtgatccc
     4081 agtggacaca gctagctgct tcaactcttt gtttttagtt ttcgcggcta cagcaaaggt
     4141 agcacctgat tgagcaatat tagaagccag cattcctggc ccattgatca tttcagaacc
     4201 cgtactgctc aattgcatag tagcgattgg cgtcattgcc caagctgtac ccgtaaccac
     4261 caaaaatggc tgcaaaccac ccatcaaaaa tggaattaac caagctgctt tgccgttaat
     4321 ccattcagcc cccattgcta taagatcgtt cagcaacgtt ccaaacggtc caatgacaac
     4381 caacgctaaa ggccccataa ttaaaaatac cagcatcggc ttagtaaaga atttaataaa
     4441 cgaaggcgaa attcgatccg caaagggctc tacataggac atgatccaaa cagtaaagat
     4501 aatcggtaaa actgatcccg cataatctgc agctaatacc ggaattccta gaaaagaaac
     4561 ggcatcgcca gtagaaaaaa gctgtgaaat atttggatga agcaaaatgc cagcaacagt
     4621 aatcgccaaa attgggttga catcaaactt tatggctgcc ccataggcta atagtactgg
     4681 cataaaataa aaggctgcat cagcaatagt attcaaaatg atgtaagttt gactactgtc
     4741 tgatagtaca ccagtcaaag tcaagacggc caaaatggct tttaccatcc cggcacctgt
     4801 tatagctgga atcatcggtg taaaggtggt agaaatgaca ctaatcaatc ttgaaataat
     4861 actttcttga ttgtctgaag aggggccctt tttaattgaa gttcccactt ccttttgaat
     4921 ttccttgaag gctgtttgga cttcattgcc aatcaccact tggtattgtc caccgctatc
     4981 caccacacta attacaccaa gtgttttttc taaggctttc gtatcggctt tccttttatc
     5041 tttgagttca aaacgcagtc tggtggcaca atgggttaag ctgaccacat tttcttttcc
     5101 cccaacctgt tgcaatacat ttcttcctac ttctctgtaa tccattattc tctctcctta
     5161 aagcagtaaa aaagaccaag tcaaaccacg ccccaatcaa cattgaagtg ttagtttgtc
     5221 ttggtcttgc ctgcataatc agtaacatgc caataacgtt attttgctgt aatccaataa
     5281 tagcacatta tgatagcgca atcaacaggg aatttaattt tttatctttt cgtttgaccg
     5341 tatagggtaa tataacagtt agatcacatc aactagggta tatctgcgta tacttactgc
     5401 taaaataagt atatctaata ttaactatac cagtaaaacc attcctacat aaccagcggg
     5461 aaattcccaa aatcgtgaaa gcatcaaaaa tgcatataac ggatcacaca acttcaacaa
     5521 ttctgctctt gggaaaaatt gatgaatttg gtgagtaaag gatggaataa acagcattcc
     5581 aacaatatag gcaacaatag tgatacttgt ttttttggta aaaatcagta agcaatagcc
     5641 aatttcaatc attccgagat ataaaggtat cgatagaatg atttgggcta gtattcttaa
     5701 aaattaacta gcaaa
//
LOCUS       DACDKM010000134         5633 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20834_2016
            SAMN12640370-rid9891663.denovo.134, whole genome shotgun sequence.
ACCESSION   DACDKM010000134 DACDKM010000000
VERSION     DACDKM010000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640370
            Sequence Read Archive: SRR10040891
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5633)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5633)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742835.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742835.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:04:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,012
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 29 of 75
            Pseudo Genes (incomplete)         :: 33 of 75
            Pseudo Genes (internal stop)      :: 19 of 75
            Pseudo Genes (multiple problems)  :: 5 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5633
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640370-rid9891663.denovo.134"
                     /isolate="E20834_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            146..1138
                     /locus_tag="IV334_002533"
     CDS             146..1138
                     /locus_tag="IV334_002533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAP8944558.1"
                     /translation="MVTIKDIAKAAGVSHTTVSRALNNSSLIKNETKQKIRQLAKEMN
                     YSPNYSAKGLVNQKKYLIGLFFSSLHKGTSSNFLADSIAGIHSVLDTNYSLSVESIDV
                     VDLTEINLQRYDGLIVMSQSDDDQPFIDYLKEKEFPFVVVNRHINDPAVINVTADDAA
                     GVMQAVDYGIGCGHTKIAYIGGKKNFRSTKEREKGVLKSLKKASIPVNLAYFLTGDYS
                     LESGLQNMEYLLSLSDLPTLVFCGNDDIAIGALRAVHLAGLRVPQNISLIGFDDSPVV
                     SYLNPPLTTVRKPLKEMCQEGTKLLLDLIEGRTIEQSKIELSTLLILRESINYL"
     gene            1198..2619
                     /locus_tag="IV334_002534"
     CDS             1198..2619
                     /locus_tag="IV334_002534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagaturonate reductase"
                     /protein_id="HAP8944559.1"
                     /translation="MKKLTKELVKKNEAPVKVLQFGEGNFMRGFIDWQIQQLNNQQLF
                     KGNVAIIQPLEQGLGNMMNQQDNLYTVILQGLSNGEIVDTSEIITVVEQMINPYDEWD
                     AYLSLAENDDLAYIVSNTTEAGIQFVETDNLTDTPPQSFPGKLTAFLYRRFKLNKAGF
                     TIIPCELIDRNGERLKEIILRYAEKWDLEPAFVEWLEKENVFCCSLVDRIVPGYPRED
                     AGVFNQRHNYEDQLMVKAEPFMLWVIEGPKELENQLPFKQAGLNVIITDDMTPYRERK
                     VHLLNGPHTAMVPLALLAELETVEEVMKDPLFSAYVDQLFNLELIPMLSLPKDELAIY
                     ADQIKERFLNPFAHHKLEAISLNSVSKFSTRLLPVFKKYIEEQNQVPPLITVSLAALL
                     LMYRGDQVKPHDDEKTISEFTDAWSDNGTAIPRLLQNAALWGEDLSQIPNVTDTVQEI
                     AAKIDQKGVRSVLQEMKGTMVHA"
     gene            2612..4111
                     /locus_tag="IV334_002535"
     CDS             2612..4111
                     /locus_tag="IV334_002535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009224330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="altronate dehydratase"
                     /protein_id="HAP8944560.1"
                     /translation="MLETLQPTMKLNRRDSVVVALTPIPKQTQLTIDQQTITTLEDIP
                     QGHKIALGDLKAGDNVIKYGYPIGHVTTDVTAGQWLHTHNVKTNLSGELDYRYEKDVH
                     PSHYLFENRTFQGYLRKNGKVGIRNDLFLVPTVGCVNGIAELIVKQFKEKHPDLGQFD
                     HITILKHPYGCSQLGKDHQNTREILADAVNHPNAGGVLVFGLGCENNTVPEFKKILGE
                     YDEERVKFLVAQDVYDEIEQGVALLEELLTAAENDQRISVPLSKLNVGLKCGGSDGLS
                     GITANPLLGAFSDYLIAQGGSTILTEVPEMFGAEQVLMARAENEEVFDSIVDLINDFK
                     QYFLSYGEPVYENPSPGNKAGGITTLEDKSLGCTQKSGSSPVVDVLQYGEKIRKPGLS
                     LLQAPGNDLVAASALASSDCQLVLFTTGRGTPFGSYVPTVKVSTNTTLFERKGHWMDF
                     NAGVLLEQPMEVILESFVQKIIAVASGEETKNEQNDVREIAIFKNGVTL"
     gene            4150..5544
                     /gene="uxaC"
                     /locus_tag="IV334_002536"
     CDS             4150..5544
                     /gene="uxaC"
                     /locus_tag="IV334_002536"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HAP8944561.1"
                     /translation="MFLSDDFLLKDDWAKKLYHSYAKNMPIIDYHCHLDPKEIYENNN
                     FSNLTEAWLGGDHYKWRLMRACGVPEEKITGNASDFEKFLAWCQTVPKIIGNPLYSWS
                     HLELKRFFGIDLLINEENAEKIWTQANEILAAPDFRRREIAKKSNVEVICTTDDPADD
                     LHYHQLLAHEEPDLKVLPSFRPDKALNIEKEGFNAWVTLLEEAADQTITTYQELIEVL
                     GQRIEYFHQHGCRVSDHALDILRYKEADETVLEEIFQKARANKLLSSDEIDAYLTETL
                     IHLTHFYHSHNWTMQLHIHAYRNCNTQMLEKLGPDTGYDGMNDLSLTIPLQKLLNRAE
                     ETDQLPKTILYSLNPNDYPAILALMGCFQKEQNGKLQLGSGWWYNDTRAGMRDQLTQF
                     ADGSALGNFVGMLTDSRSFLSYTRHEYFRRVLCEFLGEMVMRGEAPEDEQLLGSLVQA
                     ISYTNAKNYFGFFA"
BASE COUNT         1850 a         1042 c         1160 g         1581 t
ORIGIN      
        1 tcaagaatag cataattaaa aataaaaatc caagtaattt ttaacgtgtg caatcttttc
       61 atatcaaaga tctattgact tatcgtagtt atcgtaatat gatgaatgcg ttaacaaaaa
      121 gtgaaaaagt aaggaaaatg atggaatggt aacgatcaaa gatatcgcta aagcagctgg
      181 cgtttctcat acgacagttt ctcgtgcttt gaataacagc agtttaataa aaaatgaaac
      241 aaaacaaaaa atacgacaac tagctaagga aatgaattat tcgccaaact atagtgcaaa
      301 aggtttagtg aatcaaaaaa aatatctaat cggactattt ttctcaagtt tgcataaagg
      361 aacttcatca aatttcctag cagattccat tgcaggcatc cattcagtat tagacacaaa
      421 ttacagttta tctgttgaaa gtatcgatgt tgttgattta acagaaatca acctgcaacg
      481 ctacgatgga ctgatcgtta tgagtcaaag tgatgatgat cagcctttca tcgactatct
      541 caaagaaaaa gaatttcctt tcgttgttgt caatcgtcac atcaatgatc cagcggttat
      601 caacgtcact gccgatgatg cggccggtgt tatgcaggca gtagattacg gtattggatg
      661 cggacatacg aagatcgctt atattggcgg aaagaaaaat tttcgttcga caaaagaacg
      721 ggaaaaaggg gtattgaaaa gtctaaaaaa agcgagcatc cctgtcaatc tagcctattt
      781 tttaacagga gattacagct tagaaagcgg cctccaaaac atggaatacc tattatcact
      841 ttctgacctg cctactctgg ttttttgcgg caacgacgac atagcgattg gtgcattgag
      901 agctgtacat ttagctggcc tccgtgttcc ccagaacatt tctctgatcg ggttcgatga
      961 ttctccagtc gtctcctatc tcaatccgcc gcttaccacc gtacgtaagc ctttgaaaga
     1021 aatgtgtcaa gaaggaacaa agcttttact agatttgatc gaaggcagga cgatcgaaca
     1081 atctaaaatt gagctctcca ccttgttgat ccttcgtgag tcgatcaatt acttgtaaac
     1141 tacaagtaat tttatataaa aatgttaacg tgtgcataac acagaaagga agtttatatg
     1201 aaaaaactga caaaagaatt agtgaaaaaa aacgaagcac ctgtaaaagt tcttcagttt
     1261 ggtgaaggca attttatgcg tggatttatt gattggcaga ttcagcagct gaataaccaa
     1321 caattgttta aaggtaatgt tgcaatcatt caacctttag aacaaggtct tggaaatatg
     1381 atgaaccagc aagataattt atatacggtc attttacaag gactcagcaa tggcgaaatc
     1441 gttgatactt ccgagattat caccgtagta gaacaaatga tcaatcctta tgatgaatgg
     1501 gatgcttatc tatctctagc agaaaatgac gacttggcct atatcgtctc caatacaaca
     1561 gaggcaggaa tccaattcgt ggagactgat aatttaacag atactccccc acagagcttc
     1621 ccgggcaaac tgacagcctt tttataccga cgtttcaaat taaacaaagc tggctttacc
     1681 atcattcctt gcgaactgat tgacagaaac ggcgaacgtc taaaagaaat cattttacgc
     1741 tacgcagaaa aatgggattt agaacctgct tttgttgagt ggttagaaaa agaaaatgtt
     1801 ttctgttgta gtttagttga tcgcatcgtc ccaggttatc ctcgcgaaga tgcaggtgtt
     1861 ttcaatcaac ggcataacta tgaagatcaa ttgatggtca aagcagaacc attcatgctt
     1921 tgggtgatcg aagggcctaa agaattggag aatcaactac cattcaaaca ggctggcttg
     1981 aatgtcatca tcacagatga tatgactcct tatcgtgaac gtaaggtgca tttgcttaat
     2041 ggaccgcata cagcgatggt ccctcttgct ttgttggcgg aattggaaac tgtagaagaa
     2101 gtaatgaaag atcctttatt ctccgcatat gttgatcagt tattcaatct agaattgatt
     2161 cctatgcttt ctttacctaa agatgaatta gccatttatg ctgaccaaat caaagaacgt
     2221 ttcttgaatc cttttgccca tcacaaatta gaagcgattt cattaaatag tgtttcaaag
     2281 ttcagcaccc gtctgcttcc agtgttcaag aagtatatcg aagaacaaaa tcaggtacca
     2341 ccgttaatca ctgtttcact agctgcgcta cttcttatgt atcgaggcga tcaagtaaag
     2401 ccacatgatg atgaaaagac gataagcgaa tttactgatg cttggtctga taatggaaca
     2461 gctattcctc gattattgca aaacgctgcg ctttggggag aagatttgtc tcaaataccg
     2521 aatgtgacag atacagttca agaaatagct gcaaagatcg atcaaaaagg tgtccggtca
     2581 gtattacaag aaatgaaagg aacgatggtc catgcttgaa accctgcaac caacaatgaa
     2641 attaaatcga cgagacagtg tggtcgtcgc attaacccct attccaaaac aaacacaact
     2701 gacgattgat cagcaaacga tcaccactct tgaagatatc ccgcaaggtc ataagattgc
     2761 attgggtgat ttgaaagctg gagacaacgt gatcaaatat ggctatccaa tcggtcatgt
     2821 gacgacagac gtaactgccg gtcaatggct gcacactcat aatgtaaaaa ccaatctttc
     2881 tggtgaattg gattatcgat atgaaaaaga tgtccatcca agtcattacc tttttgagaa
     2941 ccgaacattt caaggttact tgcgaaaaaa tggaaaagta gggattcgta atgatttgtt
     3001 tttagtacct accgttgggt gtgtgaatgg cattgctgaa ctgatcgtca aacagttcaa
     3061 ggaaaaacat ccagatctgg gacagtttga ccatattacg attttaaagc atccttatgg
     3121 ctgttcgcag cttggaaaag atcatcaaaa tactcgagaa attttagcgg acgcagtaaa
     3181 tcatcccaat gccggaggag ttttagtatt tggattaggt tgtgagaaca atacagttcc
     3241 tgaattcaaa aaaattttag gtgaatatga tgaagaacga gtgaaatttc tagtggctca
     3301 agatgtttac gatgagatcg aacaaggtgt ggctctatta gaagagcttt taacagcggc
     3361 ggaaaatgac cagcgcatat cagtcccgct ctctaagttg aatgtcggtt tgaaatgcgg
     3421 cggttctgat ggtctatcgg gaataactgc taatccttta cttggtgcat tttctgacta
     3481 cttgattgct caaggaggca gcacgatctt aacagaagtg ccagaaatgt ttggcgcaga
     3541 acaagtattg atggcacgag cggaaaatga agaagttttt gactcaatcg ttgacttgat
     3601 caatgacttc aaacagtatt tcttgtctta cggggaacca gtttacgaaa acccatctcc
     3661 tggtaataaa gcaggtggga tcactacatt agaagataag tctttaggct gcacgcaaaa
     3721 atcaggaagc tctcctgtcg ttgatgtgct acaatatggc gaaaaaatca gaaaaccagg
     3781 gcttagttta ttgcaagctc ccgggaatga tcttgtagct gcctctgctc ttgcttcatc
     3841 cgattgccag ttggtcttgt ttaccacagg aagagggact ccgtttggct catatgtccc
     3901 aacggtcaag gtatctacga atacaactct ttttgaacgc aaaggtcatt ggatggattt
     3961 taatgcaggc gtcttattag aacaaccaat ggaagtaatc ttagaatcgt ttgttcaaaa
     4021 aatcatcgct gtagctagtg gagaagaaac gaaaaatgaa caaaatgacg ttcgagaaat
     4081 tgccattttt aaaaacggag taacactttg attttcataa aacagtataa aaagaaagaa
     4141 ggaagattta tgtttttatc tgatgatttt ttattaaaag acgattgggc gaaaaagctt
     4201 taccattcct acgcaaaaaa catgcccatt attgattatc actgtcactt agatccaaaa
     4261 gaaatttacg aaaacaacaa tttcagcaat ctgacagaag cttggttagg aggcgatcat
     4321 tacaaatggc gcttgatgcg agcttgcggc gtgcctgaag aaaaaatcac aggaaatgct
     4381 tctgattttg aaaaattttt agcttggtgt caaaccgtac caaaaattat cggaaatccg
     4441 ctttacagtt ggtcacatct tgaattgaag cgtttcttcg gtattgactt gttgataaat
     4501 gaagaaaatg cagagaaaat ttggacacaa gcaaatgaaa ttttggcagc tcctgatttt
     4561 cgccgtcggg aaatcgcaaa aaaatcgaat gtagaagtga tttgtacaac agatgatccc
     4621 gcagacgact tgcactatca tcagttgctt gctcatgaag aaccagattt gaaggttcta
     4681 ccttcattcc gtccagataa agcactcaat attgaaaaag aaggatttaa tgcatgggtc
     4741 acactactgg aagaagcagc tgaccaaacg atcaccacct atcaagaact gatagaagtc
     4801 ttagggcagc ggatagaata cttccatcaa catggatgtc gtgtttccga ccacgcttta
     4861 gacatcttac gctataaaga agcggatgaa acagtattag aagagatttt tcaaaaagca
     4921 cgagctaaca aactattatc ttcagatgaa atcgatgctt atcttacaga aacactgata
     4981 catttgaccc acttctatca ttctcataat tggacgatgc agctgcacat ccatgcttat
     5041 cgtaactgta atacacaaat gttggaaaaa ttagggcccg ataccggcta tgatggaatg
     5101 aatgatcttt ctttgactat tcctctgcaa aaattattga accgtgcaga agaaacggat
     5161 cagttgccaa agacgatcct ttattcattg aatccgaatg actatcctgc gattttagct
     5221 ttgatgggct gtttccaaaa agaacagaat ggaaaactgc agttaggttc tggatggtgg
     5281 tacaacgata ctcgagctgg tatgcgcgat caattgacac aatttgctga tggtagtgct
     5341 ttggggaatt ttgtcggcat gctgacagat tcaagaagtt tcctgtccta cactagacat
     5401 gaatatttcc gtcgtgttct ttgtgaattt ttaggcgaaa tggtcatgcg aggagaagca
     5461 cctgaagatg aacaactttt aggaagtcta gttcaagcga ttagttatac aaatgctaaa
     5521 aattactttg gcttttttgc ctaattgatt aatagtaaca gtaacgaatc aaaaacagga
     5581 tagtgaaata acggaatccg tttatccacg ttttagagaa gaagacggac caa
//
LOCUS       DACDKM010000135         5536 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20834_2016
            SAMN12640370-rid9891663.denovo.135, whole genome shotgun sequence.
ACCESSION   DACDKM010000135 DACDKM010000000
VERSION     DACDKM010000135.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640370
            Sequence Read Archive: SRR10040891
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5536)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5536)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742835.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742835.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:04:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,012
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 29 of 75
            Pseudo Genes (incomplete)         :: 33 of 75
            Pseudo Genes (internal stop)      :: 19 of 75
            Pseudo Genes (multiple problems)  :: 5 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5536
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640370-rid9891663.denovo.135"
                     /isolate="E20834_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(365..2000)
                     /locus_tag="IV334_002537"
                     /pseudo
     CDS             complement(365..2000)
                     /locus_tag="IV334_002537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294751.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(2555..3118)
                     /locus_tag="IV334_002538"
     CDS             complement(2555..3118)
                     /locus_tag="IV334_002538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HAP8944562.1"
                     /translation="MITVVHKERQEKSSSDVRLWVYLGSISTKIQYGYTDSAKKQGNV
                     KFLRITDIQEGRVNWSSVPYCDISNSKLVDLRLEENDILIARTGGTMGKSFLVKEISE
                     ESVFASYLIRIRLVEKLLSEYVDCFLDSPLYWKLLEKISYGTGQPNVNGTNLSKLLIP
                     LPPLEEQQRMTTKIEMIRRSIRRINFE"
     gene            complement(3133..4176)
                     /locus_tag="IV334_002539"
     CDS             complement(3133..4176)
                     /locus_tag="IV334_002539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8944563.1"
                     /translation="MTQRVAIMILVLLVIGLLVYYVLKFKHWKQQRIHQDIEKKLKRY
                     PIVQAAWEKAEAKQYNIPGLTETRMVVPETGENEVCQWMTPQGLAFSQDFVFISAYCY
                     DHQHHSIIHVLDRETGQPIKLLILPKRPHVGGLVYDTKRELLWLTITGSATGRVAALR
                     LIDILADTSEETGQPIAYWLTTDLSEIPQASYLTQNNDQLVSGNFTLKGEGQLTFYLL
                     PTIAEMKTAIRRKDKIQPTVTMSRKTSDKIQGVAFYGHYLLASRSYGPYTSELLVSER
                     DSPSRILKRIKFPPYLEQIVVVEDRLAVLFESGAAAYREKANPVLANVLLLDLATLLH
                     ANKRPKKIAATKK"
     gene            complement(4309..4713)
                     /locus_tag="IV334_002540"
     CDS             complement(4309..4713)
                     /locus_tag="IV334_002540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HAP8944564.1"
                     /translation="MKKMYETAMINRGGRDGEVEAPNGSMHMKIDRPGIHSEGTNPEQ
                     LFAAGYASCFNGAVQHMLEENNLESDSEVKARVSLFQLEDGGYQIGVVLEVSLPGLEK
                     DQAEKIAEEAHAFCPYSKATRGNIDVEVTIVE"
     gene            complement(4946..>5536)
                     /locus_tag="IV334_002541"
     CDS             complement(4946..>5536)
                     /locus_tag="IV334_002541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF998 domain-containing protein"
                     /protein_id="HAP8944565.1"
                     /translation="VMSDLSTPYILGLFYPKLNQMTTVISVFGDVDSPVRRAFLVWSV
                     VSGLFFVLSLPALYHLFVGTSKTLAILVVATVGLYGIGDCIFTGLFSINTNESSCNLS
                     TWIHNIGSGLGYAGFLLFPLLLVLLYRQSGQGTLSHFYLVLTVISLLIAGLYGLARIP
                     SISQFAFFKQLGFFQRLSFFFNYLPMICLSFFQLRK"
BASE COUNT         1771 a         1079 c          897 g         1789 t
ORIGIN      
        1 tttctcagaa acaaaccact cattggaaaa gacatgaatc aaatcatcac ttacttgtgt
       61 aaaatagcga cgtttaatga caaagatatc ttcattgcta tctagtgtac cttcatttct
      121 ttgtgcttaa attcaaagat aattttctca ataaagattt ttgattccag taagattcct
      181 ttactccttt tacaacatag cgttgtcctt tgttttgaaa catcttcttc ccctctttgc
      241 cattaaaaat agcccaattc tctataaaga agattaagct tcattattat aatataaagt
      301 ttaaatttgg tttattactt actttttctt attctctagt aagctaatat tgaaaaaaat
      361 gtaatcagat cattattttt atatgttgta acttcctgat aagaaaacta attaaaagaa
      421 agaggtaact acataaacta attaaaaaag ctaacttcat agttggagga taaatggaag
      481 aaacttcaat gattgtttct ccttgtgttc cttcaatcga cactagtcct attatcaatc
      541 tctctttttt gtccatttac ttttgcgact gttccccaat aatcaaatac aggaagcgtt
      601 attttttgtc ctttagattc atgtactgta tagactattt tcgaatccat cacttgataa
      661 ggcatagtat gccattcctt gccaattctt acttcatgat tctgcaaatg ttctttgtat
      721 tgtaatgctt ccgtgggtgc atagtcataa agtcctccgg gaaggagtgt cgtgtaacga
      781 ataagatttt cttgattcat taccaagtga tctctctgag tgacaacttt atgttcatag
      841 tttgcttttg ttgcagcaga tacagccaac agtaataaaa caattgccgt tatattaact
      901 acttttcttt gcttagctga tgtgctcttc atgaaaacga acgataaaat caacgagcca
      961 aacagtactt gaaagcctaa gattcgccaa ggaaattgaa tcatttgaat gggtgtattt
     1021 tgtaataaag accatggaaa aaatttggtg gataagagcc aagagactac cgcactaagg
     1081 taaatcactt tttctttgcg attaaatcct ttaaaaaaga agagtggaca gatcaaagaa
     1141 ataataccaa tgattcctac cgtaaaggtt gtcaggtcat tattcatcgc accaatcaaa
     1201 ctatccgaga catttaatgc ttggtgttgt aaggtcgttt tatctggctc attaatcgat
     1261 tgaaccatga tttgtcggaa catcggatac caaaagtatg ctgttaaaag aatcgtcatt
     1321 cctactgcct ttgacaactc cacaatcaac cggatgcttt ctttttttct tgtttcactt
     1381 actacaaagc cgcctaagac tagtaagcca acggctatta cactcataaa agtggaaaga
     1441 acatgtgtat aaatgagtaa gctcatactg actgctaatg gaattgcaca tggctttttc
     1501 tcataaatca gtgcgtaagc ataatacata actatcggta taaccgtaag tgcaatcgct
     1561 tcagcaatag cagaacgatg ataaatatca atcgtacggt acccataaaa tgtatataaa
     1621 cagctaaata taaaagccgc atcgattctt tttaaaaatt ttagtccata atgaaacatc
     1681 agcatgatag tacaaacatt caatagccaa acatataaga tataactaac gattaaatta
     1741 ttacttatcc aataaaaaat gactgccgga aaaaaggtca agtagggata aaaataattc
     1801 actccgctcc cataatgatt aaacgtcgta aaattgattg gaccagaaaa aattgaagat
     1861 aaccctttga ctctagcaaa atggaaggca atatcccccg atgtagaaat agtatgaaca
     1921 tttctaaaat aattgattga ataaagtcca gcaataattg ctaggagaat ggacaatatt
     1981 atttttttct ttacttgcat tacttaatta tatactcctt tcatggattg ctttatctag
     2041 tataacttaa attttaaata aatcgttatt tatttttact ctcacttaaa aaagcactaa
     2101 aattataaga gagggaggta atggtctgaa caccaagaat tgttccaaca tcgaattgct
     2161 taactaatga gttcaaagct taattatgtg gctcttatat ttaccgagcg tggattgcga
     2221 tctctttagc gaaacaaaca tctttcataa aaaaaacagc catatccgct ttcttggata
     2281 tgactattta ctactacttt actatctttc attcttcttg tctcacttat gataaggcgc
     2341 tgcgtatcat gagtgaatga tattaggaaa acttcataaa ataaatcttt tatacatttc
     2401 cacttctaga tggtcattca tgttagaaga atcaaactat ttttttactg tcgtaataat
     2461 attaaaacac tagaacaaat caattaaatt ccataaaata taaataatag acctagcatc
     2521 atctggtata aaatggatgc taggtccgtt atatttactc aaaattgatt ctacgaatag
     2581 atcttcttat catttctatt ttagtagtca tgcgttgttg ttcttctaaa ggtggtaatg
     2641 gtataagtag tttagataaa tttgtaccat ttacatttgg ttgcccagtt ccgtaagaaa
     2701 ttttttctaa taacttccaa tataatggcg agtctaagaa gcagtctacg tattctgata
     2761 ataatttctc aactaaccta attcgaatca agtaagaagc aaaaacagat tcctcactta
     2821 tttccttaac caagaaactt tttcccatag ttcctcctgt ccttgctatc aaaatgtcat
     2881 tttcttccaa tcttaaatca actagtttag aattactaat atcacagtaa ggcacactgg
     2941 accaatttac acgtccctct tgtatatctg tgattctcaa aaacttaaca ttgccttgtt
     3001 ttttcgccga atctgtatag ccatattgaa tcttagtaga aatacttcct aaataaaccc
     3061 atagtcgaac atcgctagat gatttttctt gtcgttcttt atgaacaaca gtgatcatct
     3121 tgagttgtcc gactattttt tagttgcagc aatctttttg ggacgcttat ttgcatgaag
     3181 caacgttgct aagtctagta ataaaacatt ggctaacaca ggattggctt tctcgcgata
     3241 cgctgcggcg ccactttcaa acaatactgc tagacgatct tctacaacaa cgatttgttc
     3301 caaatacggt ggaaatttga ttcgttttag gatccggcta ggagagtccc gctcggacac
     3361 taagagttcc gaggtataag gaccataaga acgactggct aagagataat ggccataaaa
     3421 agccacacct tgaatcttat cagatgtttt tctgctcatt gtcacggttg gttggatttt
     3481 gtctttgcgg cgaattgctg ttttcatttc agctatcgtc ggcaatagat agaaagtcaa
     3541 ctgtccttca ccttttagtg tgaagttgcc agataccagt tgatcattgt tttgagtcaa
     3601 atacgatgct tgtggaatct ctgagagatc agttgtcagc caataagcga ttggctgtcc
     3661 cgtctcttca gatgtatctg ctaagatatc gatcaaacgt aaagcggcta ctcgtccagt
     3721 agcagatcct gtgattgtca accataaaag ttcccgtttt gtatcatata ctaagccccc
     3781 tacatgtggc cgtttaggta aaatcagcag tttgatcggc tgtcctgttt ctctatccag
     3841 tacatgaata atagaatgat gctgatggtc atagcagtag gcagaaataa acacaaaatc
     3901 ttgagaaaaa gccaaccctt gcggcgtcat ccattgacaa acctcatttt cgccggtttc
     3961 cggcaccacc attcttgttt ctgttaatcc aggaatgtta tattgttttg cttcagcctt
     4021 ttcccaagct gcttgaacaa taggataccg ttttaacttt ttttcaatat cctgatgtat
     4081 ccgttgctgc ttccaatgct taaactttag gacatagtaa acaagaaggc caatgaccag
     4141 caacactaaa atcattattg ctacccgctg tgtcattttt ttgcctcctt gttctattcg
     4201 atcaatcgtc catataccgc tattaattac cgtaactaca tgtttctttt actagcgttt
     4261 ctttatagga tctttcataa tctcatttat agtaacatta agaatccctt attctactat
     4321 agtcacttcc acatcaatat tgcctcttgt tgctttagag tatgggcaaa atgcgtgggc
     4381 ttcttctgcg attttctctg cttggtcctt ctcaagtcct ggcaaagaaa cttctaaaac
     4441 gaccccgatt tggtagcccc catcttctaa ttgaaataaa gatacccgtg ctttgacttc
     4501 agaatcagac tcaagattat tttcttccag catgtgttga accgcgccgt tgaagcaaga
     4561 tgcgtatcca gccgcaaaaa gttgttccgg atttgtccct tcactatgga ttcctgggcg
     4621 gtcgatcttc atgtgcatac ttccgtttgg tgcctcaact tcgccatcac ggcctccacg
     4681 attgatcatt gctgtttcgt acattttttt cattgagatc atcctttctt tcgtcgttct
     4741 atattaagtg taacgagtat tttttaatct gacaaaggta atgcttgcat tcggcgacat
     4801 cagttagaca acatcgatga tcagtgatag aagcagttta tctatacaaa aaaagaaggg
     4861 ttctatgata tttggtgtcc ttactcgcct tgagtaacaa caccaaatat catagaacca
     4921 cttaatcagc ttcacactgc tttttctact ttcgtagttg aaaaaaactt aaacaaatca
     4981 tcggcaaata gttaaagaaa aaacttaatc gttgaaagaa gcccaattgc ttaaagaagg
     5041 caaactgact aatcgacgga atccgtgcta atccgtaaag acctgcaatt agtaaactaa
     5101 taactgttaa aacaagataa aaatggctaa gcgtaccttg accactttga cgataaagca
     5161 gaacaagtaa caatgggaaa agtagaaagc ctgcatagcc tagcccagaa cctatattat
     5221 ggatccaagt cgatagatta caagagcttt cattcgtatt gatactgaat aagccagtaa
     5281 aaatacaatc acctatccca tataaaccaa ctgttgcaac aacaagtatt gcaagtgttt
     5341 ttgatgttcc aacaaatagg tgatacaacg caggcaacga aagaacaaaa aaaagtccag
     5401 atacaactga ccagaccaag aaagcacgtc tgactggact atctacgtca ccaaacacac
     5461 taattacggt agtcatttga ttcagtttgg gataaaaaag gccaaggata tacggtgtcg
     5521 ataaatcact catcac
//
LOCUS       DACDKM010000136         5520 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20834_2016
            SAMN12640370-rid9891663.denovo.136, whole genome shotgun sequence.
ACCESSION   DACDKM010000136 DACDKM010000000
VERSION     DACDKM010000136.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640370
            Sequence Read Archive: SRR10040891
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5520)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5520)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742835.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742835.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:04:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,012
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 29 of 75
            Pseudo Genes (incomplete)         :: 33 of 75
            Pseudo Genes (internal stop)      :: 19 of 75
            Pseudo Genes (multiple problems)  :: 5 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5520
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640370-rid9891663.denovo.136"
                     /isolate="E20834_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(<1..212)
                     /locus_tag="IV334_002542"
     CDS             complement(<1..212)
                     /locus_tag="IV334_002542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP8944566.1"
                     /translation="MDFQDYEKIFESYPSILKITDLTKMLNIGKNKAYNLLKDRSIEN
                     IKIGARYKIPKKAVIEYILKIQDEGK"
     gene            288..360
                     /locus_tag="IV334_002543"
     tRNA            288..360
                     /locus_tag="IV334_002543"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:321..323,aa:Lys,seq:ctt)
     gene            522..743
                     /locus_tag="IV334_002544"
     CDS             522..743
                     /locus_tag="IV334_002544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8944567.1"
                     /translation="MRNEIANITIIRETLQNHTANEISKHTGLNLSTIKKLKSGERSI
                     EKLNLHDAICLTEFGLKNNRKNVEINIWK"
     gene            complement(1067..1312)
                     /locus_tag="IV334_002545"
     CDS             complement(1067..1312)
                     /locus_tag="IV334_002545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002412373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP8944568.1"
                     /translation="MVDNPKKRQYPLVPLSIIQSALEEDPISIKMILKHYHSYIAKLC
                     LTNGFNEAGQFITYVDEYMLRQLEIKLIEAILKFKIN"
     gene            complement(1306..1707)
                     /locus_tag="IV334_002546"
     CDS             complement(1306..1707)
                     /locus_tag="IV334_002546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010818872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HAP8944569.1"
                     /translation="MQQNYNEKSIQHQFDAYCKKVLRNETKSIRKRNINITDNEEPLT
                     NSNEGKYSQTHFDEQDVYFLFGMEILISDQKLSAAIDHLSEIRRKVILLYYFAGFNDT
                     EIGKILNMSTSGIWYQRKKAVEQLKMGYDLW"
     gene            complement(1879..3687)
                     /gene="uidA"
                     /locus_tag="IV334_002547"
     CDS             complement(1879..3687)
                     /gene="uidA"
                     /locus_tag="IV334_002547"
                     /EC_number="3.2.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003479936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucuronidase"
                     /protein_id="HAP8944570.1"
                     /translation="MLYPIMTETRQVIDLSGLWRFKLDTNDDLQEELALEPMVEKESY
                     PISVPSSYNDLFETENIRNHVGWVWYEKEVILPKKLLEERLVLRFGSATHEAKVFLNG
                     KLINQHKGGFTPFESEINQFIKDGSNRITVAVNNVIDDSTLPVGILKETIQEDGDIKK
                     ENLVNFDFFNYAGINRPVKLYTTPKDYIKDVTINTKIIDSDANVEFNIDTEWTGEILI
                     AIYDEEAQLIAEAKGKEGVIQLKNPTLWEPLNAYLYSMKVSLINENKVIDTYTEEFGI
                     RSIQVSNGKFLINNKPFYFKGFGKHEDTYINGRGLNEAVNIKDFNLMKWIGANSFRTS
                     HYPYSEEIMRLADREGIVVIDEVPAVGLHLNFMATLLDDNVEKHDTWKEIRTENAHKM
                     AIKELIERDKNHPSVVMWSIANEPDSDSEGAKEYFEPLVELTKELDPQKRPVTIVTYL
                     KSTPDICKVGDIVDVLCLNRYYGWYVSGGDLKHAKYLLKKELDGWMERCPDTPIMFTE
                     YGADTVAGLHDTVPTMFTEEYQVGYYEANHEVIDKCDNFVGEQVWNFADFETSQGILR
                     VQGNKKGIFTRDRKPKMIAHYLKKRWSSIPNFSYKK"
     gene            complement(3715..5481)
                     /locus_tag="IV334_002548"
     CDS             complement(3715..5481)
                     /locus_tag="IV334_002548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-hexosaminidase"
                     /protein_id="HAP8944571.1"
                     /translation="MTIKVDLRKKPYHLDEDAISWIEETIDAMTLEEKIGQLFVNMGS
                     SRDKNYLSSMVNQYHIGAVRYNPGTADEVYDQNYILQTESKIPLLIAANTEAGGNGAC
                     TDGTEVGVEVKIGATNDAKYAYELGRVSGIEASAIGCNWSFAPIVDINRNWRNPIISS
                     RSFGQSPDKVLELSLAYMKGIQESGILPAAKHFPGDGIDERDQHLSFSINSLSTEEWD
                     NSFGKVYSGLIEAGLPSIMAGHIHMPAYSKHFNLDLKDEDLLPATLSKELITDLLRGK
                     LGFNGLVVTDASHMLGMTGAMKRSELLPTSIAAGCDLFLFFNDPDEDFNYMMEGYYKG
                     IISEERLRDALRRILGLKAKLGLHKKEKNKILLPKQEAMEKIGLIENKSIFKEVADQA
                     ITLVKDKQDIWPIVPEKYKRILLVDVHGVKGGFGEVVAGNRPQAIDLLKQILSAKGFE
                     VEKYISPEEELKGLSQEEKIKNIAGIYAAKRPIKDLTEKYDLIINIANVNPGTVQRIV
                     WPASKGTPDIPFYIHEIPTIFVSVQCPFHLADVPQVKTYINTYDGKQETLEILVDKLM
                     GLSEFKGISPIDAYCGLKDTFI"
BASE COUNT         1719 a         1043 c          721 g         2037 t
ORIGIN      
        1 tttttacctt catcttgtat tttcaatata tactcaatga cagctttttt aggtattttg
       61 taccttgctc caatttttat attttctatt gatctatctt tcagcaaatt ataagcttta
      121 ttttttccaa tatttaacat tttagttaaa tcagtaattt tcaatatcga tggataactt
      181 tcaaatattt tttcataatc ttgaaaatcc ataaaattca ctctcaattc tgataataag
      241 tattgcactt ttttatttta ggtgttagaa taacaaagta ttaatttggg ctgttagctc
      301 agttggtaga gcagatgact cttaatcatc gggtcgcggg ttcgagcccc tcacagccca
      361 ttgggtgcca aacccttaaa tacagtgtaa acgttggtgc ttagcattta gaaaacgtat
      421 atactgtatt tttttgtttt caaaagagac tgtagtaccc ctttttttca ctaaaagccc
      481 actgtagtca ttgatatttt tgataagaaa ggaacataat tatgcgtaat gaaattgcta
      541 atataacaat aatccgagaa actttacaaa accatactgc aaatgagatc tctaaacata
      601 caggattaaa tttgagtact ataaaaaaac taaaaagtgg agaacgttca atagaaaaac
      661 taaatttaca tgatgctatt tgcctcactg aatttggtct taaaaataat agaaaaaatg
      721 ttgaaatcaa catatggaaa taattcttaa tcagctgctt ttgtcaaaag cacataggaa
      781 tttcacctct ctccaagttt acggcgagcc ttacggaagt atcattatcc caacttcaaa
      841 tcatcgcttt ttattgacta taaaaattat tttagaatgt ttatcgctcc ttctttacag
      901 taagaaatca tggcgcaact aaaatatgct ccttgaagtg ttaacgtact gatagaatgt
      961 atttaaattt caatgatcaa taggttttct gtttaaaaga atctactaat cctttaacta
     1021 ttagaagatt cttttaaaca gaataagtgg aaaggggact ttttagttag ttaattttaa
     1081 attttaagat cgcttctatc aatttaattt ctagctgtct tagcatatac tcatctacat
     1141 aggtaataaa ttgccccgcc tcattaaatc catttgtcaa acataactta gctatataac
     1201 tatgataatg tttgagtatc atcttgattg aaattggatc ttcttctaga gcagattgga
     1261 taatggatag aggaactaat ggatactgcc gttttttggg gttgtctacc ataagtcata
     1321 ccccattttt aattgttcaa ctgccttctt tcgttgatac cagataccac ttgtactcat
     1381 attgaggatt ttaccgattt cagtatcatt aaaacctgca aaataataaa gtaggataac
     1441 ctttcttctt atttccgata aatgatcaat ggctgccgat aacttttgat ctgaaattaa
     1501 tatttccatt ccaaataaga aatagacatc ttgttcatca aaatgagttt gggagtactt
     1561 accttcattt gaattcgtca atggttcctc attatctgtt atgttaatgt ttcgttttcg
     1621 aatagactta gtttcgtttc gtaatacttt tttacagtag gcatcaaatt gatgttgtat
     1681 agatttctca ttgtaatttt gttgcatgat aaatatgctc cttttttccc ctttccacta
     1741 atacaacaat cagaaagcgc ctttttttcg aaaaaactag aaaaaaatat ccttctttaa
     1801 acagaaaatc tttttataaa aaaaaggagt tggcaattaa gcagccactc cttttttata
     1861 ttccttataa aaggtaaatt attttttata actgaaattt ggtattgatg accatcgttt
     1921 ctttagatag tgagcaatca ttttaggctt tctatctcta gtaaagattc ctttcttatt
     1981 tccttgcact cttaaaatcc cttgacttgt ttcaaaatct gcaaaattcc acacttgttc
     2041 accaacaaaa ttgtcacatt tatctattac ttcgtgattt gcttcgtaat aacccacttg
     2101 atactcttct gtaaacattg ttggtactgt atcatgaagt ccagctactg tatctgctcc
     2161 atattctgta aacattattg gcgtatcggg acatctttcc atccatccat ctagctcttt
     2221 tttcaacaga tattttgcat gttttaggtc tccacctgaa acataccaac cataatagcg
     2281 atttaaacat aaaacatcga caatatctcc tactttacaa atatctggtg ttgactttaa
     2341 gtaagtaacg attgttacag gtctcttctg agggtcaagt tctttagtca attcaactaa
     2401 aggttcaaaa tattctttag cgccttcaga atccgaatct ggttcatttg caatagacca
     2461 cattacgaca ctcggatggt tcttgtcgcg ttctatcaat tccttaatcg ccattttgtg
     2521 agcattttct gtccgtattt ctttccaagt atcatgtttt tctacgttgt catctaataa
     2581 agtagccatg aaattcaaat gtaatcctac tgcaggtact tcatcaataa ctacaatacc
     2641 ttctcgatct gctaagcgca taatttcttc tgaataagga taatgtgaag tcctaaatga
     2701 attagcaccg atccatttca ttaaattaaa atctttgata tttactgctt cattcaaacc
     2761 acgaccatta atatacgtgt cctcatgctt cccaaatccc ttaaagtaaa aaggtttatt
     2821 attaatcaaa aacttcccat tcgaaacctg aatactacga atgccaaatt cttctgtgta
     2881 cgtatcaatc actttatttt cgttaattaa actaactttc atactatata agtaagcatt
     2941 tagaggctcc caaagtgttg ggttcttaag ttgaatcact ccttctttcc ccttagcttc
     3001 tgctataagt tgagcttctt cgtcatagat tgctattaga atttctccgg tccattctgt
     3061 atcgatatta aattctacat ttgcatctga atcaattatt tttgtattaa tagtgacatc
     3121 ctttatatag tctttcggag ttgtatacaa tttaacgggt ctattaatgc ctgcataatt
     3181 gaaaaagtca aaattaacca aattttcttt ttttatgtct ccatcttcct gtatcgtttc
     3241 tttcaaaatc cctacaggta aagtgctatc gtcaataaca ttgtttacag ccactgtaat
     3301 tctgttactt ccatctttta taaactgatt aatttctgat tcaaatggcg taaaccctcc
     3361 tttgtgttga ttaatcaatt ttccattcaa aaatactttt gcttcatgtg ttgcagatcc
     3421 aaaacgaaga actaatcttt cttctaataa ttttttagga agaatcactt ctttttcata
     3481 ccacacccat ccaacatgat ttctaatgtt ctctgtttca aataaatcat tataagaaga
     3541 aggaactgaa attggataac tctctttttc aaccattggt tctaaagcaa gttcttcttg
     3601 taagtcatca tttgtatcta acttaaatct ccaaagaccg cttaaatcaa ttacttgcct
     3661 cgtctctgtc ataatgggat ataacatgta aatcactcct atataattta aatattaaat
     3721 aaatgtatcc tttaacccac aataagcatc tattggactt attcccttaa attcagaaag
     3781 gcccattagt ttgtccacta atatttctaa agtttcttgt tttccatcat aagtattgat
     3841 ataagttttt acttgaggca catcagctaa atgaaacgga cactgtacag atacaaaaat
     3901 tgttggaatt tcatgaatat aaaatggaat atccggagta cctttacttg ctggccaaac
     3961 aattctttga actgtacctg ggttaacatt agctatatta ataatcaagt catatttttc
     4021 agttaaatct ttgattggtc tcttagcagc atatattcca gctatatttt taattttttc
     4081 ttcttgcgat aatcctttca attcttcttc aggtgaaatg tatttttcaa cttcaaatcc
     4141 ttttgctgac aaaatctgtt tcaataaatc aatggcctga ggtctatttc cagcaactac
     4201 ttcaccaaac ccacctttta ccccatgtac atctaccagt aaaatccttt tatatttttc
     4261 tggtacaata ggccaaatat cttgtttatc tttaactagt gtaattgctt gatcagctac
     4321 ctctttaaaa attgatttat tttcaattaa accaattttt tccatagctt cttgtttcgg
     4381 taacaaaatt ttattctttt cttttttatg caaacctagt ttagccttta agcctaaaat
     4441 tcttcttaat gcatcacgta aacgctcttc tgaaataatt cctttgtaat aaccttccat
     4501 catataatta aagtcttcat ctggatcatt aaaaaatagg aatagatcac atccagccgc
     4561 aatagaagtg ggcaataatt ctgaccgttt cattgcccca gtcattccta acatatgact
     4621 agcatcagta actactaatc cattaaaccc gagttttcct ctaagtaagt cagtgattaa
     4681 ttcttttgat aaagttgcag gaagtaaatc ttcatctttc agatcaagat taaaatgttt
     4741 tgaataagct ggcatatgga tatgacctgc cataattgaa ggtaatccag cttctattaa
     4801 gcctgagtat acttttccaa aagaattatc ccattcttct gtacttaaac tattaatact
     4861 aaaagataga tgttgatctc gttcgtcaat accgtcacca gggaaatgtt tagctgcagg
     4921 caaaattcca ctctcttgaa tacctttcat ataagctaaa ctaagttcta aaactttatc
     4981 agggctttgt ccaaaagatc gactagaaat gataggattt ctccaatttc tattaatatc
     5041 tacaatagga gcaaaactcc agttacatcc aatagccgat gcttcaattc cagaaactct
     5101 ccctaattca taagcatatt tcgcatcatt tgttgcacca atttttactt ctacaccaac
     5161 ttcggtacca tctgtacaag ctccgttacc acctgcttct gtattagcag caattaataa
     5221 aggaattttg ctttctgttt gtaggatata gttttgatca tatacctcat cagcagttcc
     5281 tggattatat cgtacagctc caatatggta ctgattgacc atactactta agtaattttt
     5341 atcacgacta gatcccatat taacaaataa ttgacctatt ttttcttcta aagtcatagc
     5401 atcaatcgtc tcttcaatcc aagaaatagc atcttcatct aaatgatatg gtttttttcg
     5461 taagtctact tttatagtca ttttctttct cctctactta ttagtaagat ttttccaaaa
//
LOCUS       DACDKM010000137         5143 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20834_2016
            SAMN12640370-rid9891663.denovo.137, whole genome shotgun sequence.
ACCESSION   DACDKM010000137 DACDKM010000000
VERSION     DACDKM010000137.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640370
            Sequence Read Archive: SRR10040891
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5143)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5143)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742835.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742835.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:04:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,012
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 29 of 75
            Pseudo Genes (incomplete)         :: 33 of 75
            Pseudo Genes (internal stop)      :: 19 of 75
            Pseudo Genes (multiple problems)  :: 5 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5143
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640370-rid9891663.denovo.137"
                     /isolate="E20834_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            367..555
                     /locus_tag="IV334_002549"
     CDS             367..555
                     /locus_tag="IV334_002549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8944572.1"
                     /translation="MELIVFTNNGQTYHFYEVENFKPTTTGFSFIYTGKATGVTRSAT
                     FNNTSTAGYALAEVQNES"
     gene            545..805
                     /locus_tag="IV334_002550"
     CDS             545..805
                     /locus_tag="IV334_002550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8944573.1"
                     /translation="MNHEKFIEKCKAIVRERIENEIADLSGAVPEFSIFIVWSCKTLQ
                     NSKALVSASLKGAPYFEITLNGDKGEIYVDTYLKKSNECIKV"
     gene            846..1073
                     /locus_tag="IV334_002551"
     CDS             846..1073
                     /locus_tag="IV334_002551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002370649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8944574.1"
                     /translation="MRNYWYVSLTNEYPRTIGDCSVRVVRSVQIKGKYSIVEMLREAT
                     PNEVDKCKLIYCGHGYWKDEYIQYNIERYIK"
     gene            1138..1710
                     /locus_tag="IV334_002552"
     CDS             1138..1710
                     /locus_tag="IV334_002552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8944575.1"
                     /translation="MKLKDYIREGYNVVTAPNLAYKIQEDYPNALVFTDRALDAIPIG
                     KLLVDRFYSNNPAVLSAKPLQNAYAIERFTCEFTGYETVVPMTGETGRKVIQQIKKRI
                     AELTLDDGSNTNLLEIKLRDTDSVPEVYYKGQRMDEMPIGLVDITYHWDTEEYECQVP
                     KGANDITIEYYDSHNNKYLDRKIIGHKRDM"
     gene            1711..2022
                     /locus_tag="IV334_002553"
     CDS             1711..2022
                     /locus_tag="IV334_002553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HAP8944576.1"
                     /translation="MIDVSSKQARARFYGSTEWRKLRSQCLERDHYECQWCKAEGRLT
                     TQHDSILEVDHIKELEYHPDLALDINNLRTLCKDCHNKRHNRMKYRGQQKKRKWDDEW
                     W"
     gene            2013..2273
                     /locus_tag="IV334_002554"
     CDS             2013..2273
                     /locus_tag="IV334_002554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8944577.1"
                     /translation="MVVRKTANRDDVKRAEAIAMVDKAIDKLVLFKSQLIEGTTTVED
                     ISINNRLIGNANEVTGTFNINVDIDYTLSPFAHIVESNEQDG"
     gene            2434..2793
                     /locus_tag="IV334_002555"
     CDS             2434..2793
                     /locus_tag="IV334_002555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8944578.1"
                     /translation="MADLQERNRLVSAEERRLNKLFKDIDKDKKKVVSGLITQAARLK
                     ILLDEMWIDISEKGDYELFSQSENQKPYERERPVAKQYNSRDQAYQRVIKQLTDYLPE
                     EKREPTKNAALDGSDLL"
     gene            2790..4433
                     /locus_tag="IV334_002556"
     CDS             2790..4433
                     /locus_tag="IV334_002556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HAP8944579.1"
                     /translation="MTTLKPFYFDEYVDLYEKGVIPFNKERIQLITYLKNEVLVRDDI
                     YFDEEMIQKFIKYTEKNFFPLAKYQKFITPFIFCYQKEDDEVFFDEILNSIARGGGKN
                     GFMSARDSFFISPLYGVRNYDVTITANSEKQGKVSFKEVYEMVQMRKLEKQFYLTKMA
                     IVNNVTNSIFSYRTNNPKTMDSARDGCLEFDEIHQFESSDLVDIQRSGLGKIKHPRTF
                     YNGTNGHVREGFYDKMLERAQKIFSGENKNDRLFPFICKLDNIDEMDNPKMWSKANPM
                     FEEDSAYSKRLFSTVMKEYKKLEDEPSGRREFVVKRMNFTEGDAESDITTHEKLMATK
                     QELPNLNGRSCVAGFDYASIRDFATVGLLFKIDDKFIWQQHSFARKKFLDTFKLKAPI
                     KDWEKKGLVTIVDEPSIDPRHLIDWLNEKRKIHQIEIVCADGFRMDLLKPLLEKESYN
                     FEFIRNIRGVQSKVAPIIEDGFANERFIFGDDPMMRWYTNNSFIKEDKLGNKTFLKKE
                     PVRRKTDGFHAFLAALYKREEITDMDYEEAFDMLDEIEF"
     gene            4468..>5143
                     /locus_tag="IV334_002557"
     CDS             4468..>5143
                     /locus_tag="IV334_002557"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8944580.1"
                     /translation="MKKRYFLFVTIFIIIAICVLTVFLPQITEYLNYRIFTTKVDDSV
                     TSLSRLEFIKICASVFGPILTVMVFVNTLNMQKKTEQKQNELEVKQAKAENERAKKEL
                     LTQIDREFYFLLNMFIEIQKDQNAKQAVIALQKLAIFNEKGFNQDYAFTSKDLSDSEI
                     GSTIEQSGPLKGTVEYIKFETFDVSKSMGNYQTKTKDVATSLHDQKVAILNEQYEKMQ
                     IYLGRYF"
BASE COUNT         1833 a          713 c         1082 g         1515 t
ORIGIN      
        1 cttctaaatt gttcttatta tctatcatca gttgctgtct attgtcatta tgtcactgtg
       61 gtggaaaggt gtatcgctca tctaaaattt aggtgcaaac tgcaaggttc gattccttgc
      121 cagtgacttt ggtaaccgag gcatcggcgg tttaaaaata taggggtgcg caatttcgta
      181 cgcgttttgt gcatcttgta ggttgctatt acataattgg ttgggttaga ttgagttttg
      241 ggattcggta caaatgaatc gtcaaatgac tcaagcacag gatcggaaac gtccctgcct
      301 gtgcattaca tattagatca ctctttgagt ggtcttttta ttttaaaaaa gagaggattt
      361 tgaaaaatgg aattgattgt atttactaat aacggacaaa catatcattt ttatgaagtg
      421 gagaatttta agccaacaac aacaggattt agttttattt atactgggaa ggctacggga
      481 gttactcgtt cagctacgtt taacaataca tcaacagctg gttatgcgct tgcggaggta
      541 caaaatgaat catgagaagt ttatcgaaaa atgcaaggct attgtacgtg aaagaattga
      601 aaatgagatt gctgacctaa gtggagcagt gcctgagttt agtattttca tagtctggtc
      661 atgcaaaaca ctgcaaaata gcaaagcatt agtcagcgct agcttaaaag gagcaccgta
      721 ttttgaaatt acgttgaacg gggacaaagg tgaaatctat gtagatactt atctcaaaaa
      781 atcaaatgaa tgtatcaaag tctagcaggt gctaggcttt ttctttacat aaaggaggct
      841 gcataatgag aaactactgg tatgtatcac taactaatga atatcctcga accattggtg
      901 attgttcagt gcgtgttgtg cgttctgtac aaatcaaagg gaagtactct attgtcgaaa
      961 tgctaagaga agctacacca aacgaagtgg ataaatgcaa gctgatatat tgcggtcatg
     1021 gctattggaa agacgagtat atccaataca acattgagag gtacatcaaa tgaatgataa
     1081 ctatgattac atcaagttgt tagcaagtaa ttattagatg tttttaggag gaaaactatg
     1141 aaattaaaag attatatcag agaagggtat aacgttgtaa ctgcacccaa tctagcttac
     1201 aaaattcaag aagattatcc caatgcttta gtgtttactg atagagcttt agatgccatt
     1261 cctataggga agttattagt agatcgtttt tatagcaata atccagctgt tcttagtgct
     1321 aagccacttc aaaacgctta tgctattgaa cgctttactt gcgagtttac tggatatgag
     1381 acggtagtac caatgacagg tgagacagga agaaaagtaa ttcaacagat caaaaagagg
     1441 attgctgaat tgacgttaga tgatggaagt aatacgaacc ttctagaaat caaactaaga
     1501 gatactgact cagtaccaga agtttattat aaaggtcaac gaatggatga gatgccgata
     1561 ggtcttgtcg atattactta tcattgggat acggaagaat atgaatgcca agtaccaaaa
     1621 ggagctaacg acatcactat tgaatattat gattcacata ataacaagta cttagataga
     1681 aaaatcattg ggcacaagag agatatgtaa atgattgatg tttcaagtaa acaagcaaga
     1741 gctaggttct atggatcaac tgagtggagg aaacttagaa gtcaatgctt agagcgagat
     1801 cattatgagt gccaatggtg taaagcagag ggtagactaa caactcaaca tgattcaatt
     1861 cttgaagtgg atcatataaa ggagctggag tatcatccgg atttagctct agatataaac
     1921 aacctaagaa ccttatgcaa agattgccat aacaaaaggc acaaccgtat gaaatatcgt
     1981 ggccaacaaa agaaaagaaa gtgggatgat gaatggtggt aagaaaaaca gcaaacagag
     2041 atgatgtcaa acgagcagaa gcaatcgcta tggttgataa ggctattgat aagttggttc
     2101 tatttaagag tcagctcatt gaaggaacaa caactgtaga ggatatatct atcaacaata
     2161 gactaatagg taacgctaat gaagtaacag ggacatttaa tattaatgta gacattgatt
     2221 ataccttgag tccatttgca catattgtag aaagcaacga gcaagacggc tgataaatca
     2281 gtataaagcg aagatatata gagtgaaata cccccggtca aaagattttg agaagaaata
     2341 aaatttttgg gaaccggtgg atggggtcaa ctctgcaaat aaatgatgct attttcacac
     2401 gaccccctcc ccacgtagaa aggaagtgag ttgatggctg atctgcaaga gagaaatagg
     2461 cttgtttctg ctgaagaaag gcgtctaaat aaattgttta aggatattga taaggataaa
     2521 aagaaggtcg tttctggttt gattacgcaa gcggcacgct taaaaattct tcttgatgaa
     2581 atgtggattg atatttccga aaaaggtgat tatgagcttt tctctcagtc agaaaatcaa
     2641 aaaccttacg agcgggaacg accagttgct aaacagtata attcgagaga ccaagcatat
     2701 caacgtgtaa ttaagcaact tactgactat ttgccagaag aaaagcgcga acctacaaaa
     2761 aatgcagcgc tagatggtag tgatttgctg tgacaacatt aaagccattc tattttgatg
     2821 aatacgtaga tttatacgaa aaaggggtaa tcccttttaa taaagaaaga attcagttaa
     2881 ttacttattt aaaaaacgaa gtgctagttc gggatgatat ctattttgat gaagaaatga
     2941 ttcaaaagtt tatcaagtac actgaaaaaa atttttttcc tttagctaaa tatcaaaaat
     3001 ttatcacacc tttcattttc tgctatcaaa aagaggacga cgaggtgttt tttgatgaaa
     3061 ttctaaatag tattgcgcga ggcggcggga aaaatgggtt catgtcagca agagactcat
     3121 tttttattag ccctctctac ggtgtcagaa attatgatgt aacgattaca gcgaattctg
     3181 agaagcaggg aaaggtatct tttaaagaag tttatgaaat ggtacaaatg cgcaaactgg
     3241 aaaaacaatt ttatttgact aagatggcta tagtcaacaa tgtaactaat tcgattttta
     3301 gttataggac aaacaatcct aagactatgg atagtgcaag agatggctgc ttagaatttg
     3361 atgaaatcca tcagtttgaa tcttcagacc tagttgatat tcagcgtagt gggctaggga
     3421 aaataaaaca tcctcgaact ttttataatg gtacaaatgg tcatgttaga gaaggttttt
     3481 atgacaaaat gttggaacga gcacaaaaga tttttagtgg agagaacaag aatgatcgct
     3541 tgtttccctt tatttgtaaa ctggataaca ttgatgaaat ggataatcct aaaatgtggt
     3601 caaaggctaa tccaatgttc gaagaagatt cagcatattc aaaacgtctg ttttctacag
     3661 tgatgaaaga atataaaaaa ctagaagatg agccatcagg taggcgtgaa tttgttgtta
     3721 aacgaatgaa ttttacagaa ggtgatgcag aatcagatat tacgactcat gaaaaattaa
     3781 tggctactaa acaagaattg ccaaatttga atggtcgttc ttgtgtggct ggatttgatt
     3841 atgcaagtat tcgtgatttt gcaacagtgg gtttgctttt taaaattgat gataaattta
     3901 tctggcagca acatagtttt gctaggaaaa agtttctaga tacattcaaa ctgaaagcgc
     3961 ctattaagga ttgggagaaa aaaggtttag tgacaatcgt tgatgagcca tcaattgatc
     4021 ctcgtcattt aattgattgg ttaaatgaaa aaagaaaaat ccatcaaata gaaatcgttt
     4081 gtgccgacgg atttcgtatg gatttgctga aacctcttct agagaaagaa tcatataatt
     4141 ttgagtttat cagaaatata agaggtgtgc agtcaaaagt tgctcctatt attgaagatg
     4201 gttttgcaaa tgaacggttt atttttggtg atgatcctat gatgcgttgg tatacaaata
     4261 attcgtttat caaagaagac aaattaggga ataaaacatt tttgaaaaaa gagccagtaa
     4321 ggcgtaaaac agatgggttt catgcttttt tggcagcttt atataaacga gaagaaataa
     4381 ctgatatgga ttatgaggaa gcatttgata tgttggatga gattgaattt taaggtatac
     4441 tccttattga aaggggtagt tatagaagtg aaaaagagat attttctatt tgttacaata
     4501 tttataataa tagcaatttg tgttttgaca gtatttttac cacaaattac tgagtattta
     4561 aattatcgga tatttaccac aaaagtagat gatagcgtta ctagtttaag taggctagaa
     4621 tttataaaaa tatgtgcatc tgtgtttggt cctattttga ctgtaatggt atttgtgaat
     4681 actttaaata tgcagaaaaa aacagagcaa aagcaaaatg agcttgaagt aaagcaagct
     4741 aaggctgaga acgagagagc aaaaaaagag ctgttaacac agatagatcg tgagttctat
     4801 tttttattaa atatgtttat tgaaatacaa aaagatcaaa atgccaaaca agctgttatt
     4861 gctttacaga agttggcaat ttttaacgaa aagggtttta atcaagacta tgcatttact
     4921 tctaaagatt tatcagatag tgaaattggg agtacaattg aacaatcagg accgctgaag
     4981 ggcacagttg aatatataaa atttgaaaca tttgatgtaa gcaagtcgat ggggaattat
     5041 caaacaaaaa caaaagacgt tgctacaagt ttacacgatc agaaagtagc tatattgaat
     5101 gaacaatatg aaaagatgca aatatatctt ggacggtact tca
//
LOCUS       DACDKM010000138         5075 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20834_2016
            SAMN12640370-rid9891663.denovo.138, whole genome shotgun sequence.
ACCESSION   DACDKM010000138 DACDKM010000000
VERSION     DACDKM010000138.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640370
            Sequence Read Archive: SRR10040891
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5075)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5075)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742835.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742835.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:04:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,012
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 29 of 75
            Pseudo Genes (incomplete)         :: 33 of 75
            Pseudo Genes (internal stop)      :: 19 of 75
            Pseudo Genes (multiple problems)  :: 5 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5075
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640370-rid9891663.denovo.138"
                     /isolate="E20834_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(1135..1800)
                     /locus_tag="IV334_002558"
     CDS             complement(1135..1800)
                     /locus_tag="IV334_002558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP8944581.1"
                     /translation="MNTGEKIRTYRRNCKLTQKELGEKIGVSDKTISSWENSRTMPDL
                     EMLSLLHHTLGLPIDFPSIESATESIEPTLPDEPLVNKKSYSSKQPFSLKKTLSFFLL
                     VFCLVYLFLINYQYKNETIDRFNPFLKPQIGYTTLPVGYVDNSIERWVCNDIFGHGTW
                     LSFHAGEVEKEKNHAVIKHKGKHVMNVRLLTWEAIPSDYQEILSQDYDDALKRSPQEP
                     DRH"
     gene            1926..3962
                     /locus_tag="IV334_002559"
     CDS             1926..3962
                     /locus_tag="IV334_002559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1430 domain-containing protein"
                     /protein_id="HAP8944582.1"
                     /translation="MIIKKKFTVLLTIIVTGIMSGFALVSINKQVLYRTNEFFHQTDT
                     YTPIRLPQHFIGQEEYEQLIMVFENVSSETELSYMKKTVNESNEVDTSWNFKIQPKLE
                     MTLYYQGDVKSSHEKVPYSNTSFRVTSIEESIKIKDFEGEFFLMTREKEAYERFISLF
                     TDRFNQAFHTDYATNIFEDFENSYSYSVISNTDVYNYRVYLTIGLILLFLLLSSFFLL
                     MNKEISLLLNNDFSLHQVLWCLLGKKKIIITFIIAGIIGGFIFMYYKEQFLYLFQMYV
                     FLFSGIYGISLISLLLVEKIASLREAFNYIQYILFTFGLLFSLLLTVANVELATIVFS
                     SSQLAVSHEPPKEIAGEDYHVFYPVTIGKNHVDFIYSNRFASAADQELYETLNKNGSI
                     LVNVSDYLNEENEQFGRGIKINLNYLKKFPLIDVSGRLIQITEKETHPVILIPERYRH
                     TDLKNDLAQITEYYQEISEKEPKFLFIKNNQPIYTFIPSIPWIEHYPVVEILTLKNSM
                     YLERNILSGEIYPPLKIKIGSLSKERLFELIEESQLRDNLQLSFPYTQTEEIAVKVLS
                     RSFHYLIQIFLLTFFSFFLLSYVMLCIYFVYNCRKIAIFRSSGYSLFETYKDFFMMNL
                     IKWGTTSVIFLFLIEREPKYLFNIFFFSFIDFILSVVFILSTEKKSQLLLMNGG"
     gene            3966..4604
                     /locus_tag="IV334_002560"
     CDS             3966..4604
                     /locus_tag="IV334_002560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP8944583.1"
                     /translation="MEPLIVIKGAKKIIRNKTILHLDRFTADAGDLVTVVGESGAGKS
                     TLLNILGLNDQFSSGQFYLFDQETKQLTGREKRLIKRNTISFLFQDFGLIEYETIDFN
                     LDIGLKFSNLNVSDKRTAKYEALKKVNINKKLSTPIYTLSGGEKQRIACARVLLKNSK
                     IILADEPTGSLDKRNKDKVVEILSQQASQGTLVIVVTHDPYLTKIGNKKIML"
     gene            4632..5060
                     /locus_tag="IV334_002561"
     CDS             4632..5060
                     /locus_tag="IV334_002561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8944584.1"
                     /translation="MKKAVIICALGIGFVGGNAMSSEVEANSVHGEIYYKSLKPGEIH
                     FDAKGNIIKSNLPVDREISRTAINHSSGRWVYYSDTTCTRKTGHSNHYSRVYNRHGAK
                     AKVGNSIRSANATKGVWAYASATGNRTDTFSCWYNPTGWY"
BASE COUNT         1671 a          778 c          935 g         1691 t
ORIGIN      
        1 ccttgtttgt tttagtttaa actagtgtgt aatttaaaaa tctatatgga agggtctgtg
       61 gggtaatact taaataaagg ctaggaacct gataacatca aggttcctag cctttttata
      121 ataaaatgaa gcgaaaataa tttcttttaa agagccgaga ttatcagcta tataagagtg
      181 aatttgatgc aaaaaacaga gccgaaacag tcttggtgtt cgtagctctg cttatatgtt
      241 tcgaatttaa gacaaagagt tggcaaaaga tgttgataag gaagataaga taactgtgtt
      301 atataaacta ggtaactaac tgtaacaagc taattttaag agattcttat tagaagacgt
      361 ttaggaatat aactaagaaa atgattatac aggtaattgt catttcaata atgttttcta
      421 attttcgcgt ctttgtttta agaaaaaggc cagaatcagt tggaaaaaag ttcgtacgat
      481 agtgaagagg ggcaaaattt cctactaata ttcccactaa caagagataa aacggccagg
      541 ttggtgtgat tcctctataa aaaaatgccc atgctaccga aatcccatag attaacaact
      601 gaacaatttt tacaatgaaa aaattgttct gccattactc aaagtctctt cctccttgta
      661 tcgtttattt gtttcatttt atcattatga taggcgcttg aacaatccat tttgtatttt
      721 attgagtact cttggataaa ctaccagcga ttatcatatt tatttatgtt actgtaagcc
      781 atcatataaa agtaaatgag cagatcatag aaatataaaa aagaactaaa attgatctaa
      841 attatgtgca gcttgactac gtaccaatct atattactta ctagattggt cgttaatcta
      901 ttcggatcga atgagcaaaa tatttttgtg aatgtccatc cttgattatg attccaaaaa
      961 gaatcattgg gaggctgtgg cgtaaatatt atactataag ccagaaatac ctatcattct
     1021 gaactttttt atttaggaac tttcatctca aggctttttc aattaatttg tacccctgtt
     1081 ttttggataa gatcatgtgc attgattttt tctatctcta cgttcatctc ctacttaatg
     1141 cctatctggc tcctgagggc ttctttttaa ggcgtcgtca tagtcttggc tgaggatttc
     1201 ttggtaatct gagggaatgg cttcccaagt gagcaggcga acgttcatga cgtgttttcc
     1261 tttgtgtttg attacagcat ggtttttctc tttttctacc tctccagcgt gaaaagacag
     1321 ccatgtacca tggccaaaga tatcattaca tacccaacgt tcgatggaat tgtccacgta
     1381 tcctacaggg agtgtggtat agccgatttg tggtttcaaa aaaggattga atcgatcgat
     1441 cgtttcattt ttgtattgat aattgataag aaaaagatag accaaacaaa agacgagcag
     1501 aaagaaagat agtgtctttt tgagtgagaa gggttgtttt gatgaataag atttcttgtt
     1561 aacaagaggt tcatctggca atgttggttc tattgattcg gtagctgatt caatagaagg
     1621 aaaatcaatc ggcagaccta aagtatgatg taaaagtgac agcatttcaa ggtctggcat
     1681 tgtacgagaa ttttcccaac tagaaatcgt tttatcggaa acaccaattt tttcgcctaa
     1741 ttctttttgc gtaagcttac aatttctgcg gtaagttcgt attttttctc cggtgttcat
     1801 cttcttctcc tttcgattct acagtatata gatttattct acatgaccta tacacttgat
     1861 agatacgttt ttttgatgaa aaacggtatg attttatcaa agaatcattt aagtaaggga
     1921 gagtggtgat aatcaaaaaa aagttcacag ttttactcac aatcatagtg accggtatca
     1981 tgagtggttt tgccttggtt agtataaata aacaggtact ctatcggaca aatgaatttt
     2041 tccatcaaac agatacttat actccgattc gattgccaca acattttatt gggcaagaag
     2101 aatatgagca gctaatcatg gtatttgaaa atgtctcatc agaaactgag ctttcttata
     2161 tgaaaaaaac agtaaatgaa tccaatgaag tagatacatc ttggaacttc aagatacagc
     2221 caaagcttga aatgacattg tattatcaag gggatgtcaa atcttctcac gagaaggtac
     2281 cctattctaa tacctccttt cgcgtaacgt ccattgaaga atcgatcaaa ataaaagatt
     2341 ttgaaggtga atttttcttg atgacgagag aaaaagaagc ctatgaacgg ttcatttcac
     2401 tattcactga tcgattcaat caagcatttc acactgatta tgccactaat atttttgaag
     2461 actttgaaaa tagctattcg tattcggtta tttcaaatac agacgtatat aattatcgag
     2521 tgtatttaac tattggattg attcttttat tccttttgtt atcatccttt ttcttattga
     2581 tgaataaaga aatcagttta ttgttgaata atgacttttc attacatcaa gtattgtggt
     2641 gtttattggg gaaaaagaaa atcattatta cattcataat tgcaggaatc attggtggtt
     2701 ttatttttat gtattataag gagcaatttc tttatttgtt ccagatgtac gttttcttgt
     2761 tcagcggaat atatggtatc tctttgattt ctcttttatt agttgaaaaa atcgcttcgt
     2821 taagagaagc gttcaactat atccaataca ttctttttac atttggattg ttgttttctc
     2881 ttcttttgac cgttgccaat gtcgaattag caactatcgt tttttcttct tcacagttag
     2941 ctgtttccca tgaaccacca aaagaaatcg ctggtgagga ctatcatgta ttttatccag
     3001 taaccatcgg gaaaaaccac gtggatttta tttattccaa tcgttttgca tcagcagcag
     3061 atcaggaatt atatgaaact ttgaataaaa acggaagtat attagtcaat gtatcagatt
     3121 acttgaatga agaaaatgaa cagtttggca gagggataaa aataaatctg aattatctta
     3181 agaaatttcc tttgattgat gtctctggaa ggttgatcca aataaccgaa aaagaaacgc
     3241 accctgttat attgatccct gaacgatata gacatacaga tttaaaaaat gacctggcgc
     3301 aaataacaga atattatcaa gaaatcagtg aaaaagaacc gaaatttcta tttataaaaa
     3361 acaatcaacc catttataca tttattcctt ctatcccctg gatcgaacat tatccggttg
     3421 ttgaaatact gacattgaaa aacagtatgt atttggaaag aaatattctc agcggagaaa
     3481 tctatccacc gttaaagatc aagataggct ctttgtcgaa agaaaggtta tttgagctga
     3541 ttgaagaaag tcagttacgt gataacttgc agttatcatt tccgtatact cagactgaag
     3601 agatagctgt caaagtgtta agcagatcat ttcattactt gatacagata tttttactca
     3661 ctttcttttc tttcttcttg ttgagctatg tcatgttatg catatatttt gtctacaatt
     3721 gtcggaaaat cgctatcttt cgttcaagtg gttattcgct ctttgaaacg tataaagatt
     3781 tcttcatgat gaacctcatc aaatggggaa cgacaagtgt aatcttcctt tttcttatag
     3841 aaagagagcc taaatatctt tttaatattt ttttctttag cttcattgat tttattcttt
     3901 cagttgtttt tattttgtct acagaaaaga aaagtcagtt attgctgatg aatggaggtt
     3961 aacaattgga gccgttgata gtgataaaag gtgcgaaaaa aatcattcga aacaaaacga
     4021 ttcttcattt ggatcgtttc actgcggatg ctggggatct tgtgactgtt gttggagaaa
     4081 gtggagcagg taaaagtacg ttattgaata tattaggatt gaatgatcag ttcagttcag
     4141 gacaattcta tttgttcgat caagagacga aacaattaac tgggagagag aagcgcttga
     4201 taaaaagaaa taccatatct tttctttttc aagattttgg gttgattgaa tatgaaacaa
     4261 tcgattttaa tttagatatt ggattgaagt tttcgaattt aaatgtttca gataagcgaa
     4321 cagcaaagta tgaggcattg aaaaaagtaa atataaacaa gaagctaagt acgcctatct
     4381 acacattatc tggtggtgag aagcaaagaa tcgcctgtgc gagagtctta ttgaagaata
     4441 gcaaaatcat tttagccgat gaaccgactg ggtcattgga taaaagaaat aaggataaag
     4501 tagttgaaat cttatcgcaa caagcttccc aaggtacgtt agttatcgta gtcacgcatg
     4561 atccatatct gaccaaaata ggaaacaaga agatcatgct ttaaaaatca aaaaggaaag
     4621 gatgtcgttt tatgaaaaaa gcagttatta tttgtgcact agggataggt tttgttggag
     4681 gaaacgctat gtcatctgaa gtggaagcaa attcagttca cggtgaaatc tattacaaat
     4741 ctttaaagcc aggagagatt cattttgatg ctaaaggtaa tattattaag tcaaatttac
     4801 cagtagaccg ggaaatttct agaacagcaa ttaatcatag ctctggaaga tgggtttatt
     4861 atagtgatac aacttgtacg agaaaaacag gtcattctaa ccactatagt cgtgtctata
     4921 atcgtcacgg tgcaaaagcg aaagttggaa actctattcg atcagcaaat gcaacaaaag
     4981 gagtatgggc ctatgcaagt gcgacaggaa atagaactga cacattctca tgttggtaca
     5041 atccaaccgg ttggtattaa gtatccaaaa gttta
//
LOCUS       DACDKM010000139         5059 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20834_2016
            SAMN12640370-rid9891663.denovo.139, whole genome shotgun sequence.
ACCESSION   DACDKM010000139 DACDKM010000000
VERSION     DACDKM010000139.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640370
            Sequence Read Archive: SRR10040891
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5059)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5059)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742835.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742835.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:04:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,012
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 29 of 75
            Pseudo Genes (incomplete)         :: 33 of 75
            Pseudo Genes (internal stop)      :: 19 of 75
            Pseudo Genes (multiple problems)  :: 5 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5059
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640370-rid9891663.denovo.139"
                     /isolate="E20834_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(2..1027)
                     /locus_tag="IV334_002562"
     CDS             complement(2..1027)
                     /locus_tag="IV334_002562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAP8944585.1"
                     /translation="MVGIRDVAKRAGVSIATVSRVLNEDPTLSVTDETKRKIAESVNF
                     YHYVKKSPVAKNLTNIALITTVSEVDELEDPYFRTIRRGIQVEAEQSKVNLKKIIRLS
                     ETPLEQDDLLKCQGVLIIGQVLSSVIEEVSAINSNVVVIDDPSIEAEVDAVYTDLTKA
                     TNQHLDRLYKKGHRNIAFIGGGRVRLDSQGNKHVSDDDYRKIAYETWMKQKGLEKYSQ
                     IYLEGWTTLDGMQAADLLLKKHAKDKLPTAIMVGNDPIAVGVYRSLQKNGLHIPEDIS
                     IVSFDNIEVAEFLTPALNTVNVNTEEIGKLAVRMVNERIKEVRNVSIKVIVSNNIIVR
                     ESEKNID"
     gene            complement(1272..2543)
                     /locus_tag="IV334_002563"
     CDS             complement(1272..2543)
                     /locus_tag="IV334_002563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HAP8944586.1"
                     /translation="MKLSTQEKQLKNVSLKIPKNKISIFTSVSGSGKSSIVFDTITQE
                     AGRQLNDTYDSFTRLFLPKYSRPEVDEIDNLSTAIVVNQKKIGGSARSTLGTITNINA
                     LFRVLFSCFATPSLGYANAYSFNDPSGMCSVCQGIGKTIAFLIGLLYFSAIDNADFVF
                     SDPFTAEVLSGIEEQAKNHGYFVLVHNVNSAEDVKTIQRNWRFEGFIVVGVRVKEFLE
                     LNNVFDGPVVYIDTHLSKETLRELEQQMNRLFVNTNDYQASTLAVEHLIHYGHEKIAF
                     LSFDYIVGEPSVIQERHQAYIDTLKKYHLTFKSHWIYNNSEFERIYQELAEFTAIVVT
                     GDYLAAKLVKFLKNKNKRVMEQLSIIGFDDITFAELMDPALTTIRLNPGNKGAIAFHQ
                     LIGLAKQEEIKNQVLLLEGELISRDSVHKIK"
     gene            complement(2827..3774)
                     /locus_tag="IV334_002564"
                     /pseudo
     CDS             complement(2827..3774)
                     /locus_tag="IV334_002564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016634498.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ring-cleaving dioxygenase"
     gene            3786..4259
                     /locus_tag="IV334_002565"
     CDS             3786..4259
                     /locus_tag="IV334_002565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HAP8944587.1"
                     /translation="MRIKPIKDGEQLLFSSEENKPLYLDYVNHNLKIIIKENNLKHIT
                     LHGFRHTHCSLLFESGASVKEVYVRLGHTDIKTTMDDIYTHIAKKQTEETAIRFADFM
                     SSTEEKQGSLQKVKSMVKNEKSRLSLLDSLLNVELSVFFLFRSVCVFETVLILFF"
     gene            complement(4432..4986)
                     /locus_tag="IV334_002566"
     CDS             complement(4432..4986)
                     /locus_tag="IV334_002566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HAP8944588.1"
                     /translation="MSYNVQPLRTNEEIQDFQFWLRRTSNPERDSFLFLFGINNGLRM
                     SDIIGLKVGDIRGKSKPIIVEHKTGKRKPIFIDNLREEILLYTEGKEENDWLFPSRQQ
                     GRHITRDRVYQIYADIAEKLGRDDIGTHTLRKTFGYHYYKKTRDIATLMFIFNHSSQA
                     ITKRYIGITEDEIGASLRGFKLGV"
BASE COUNT         1603 a          959 c          747 g         1750 t
ORIGIN      
        1 tttaatcgat atttttttca ctttctctaa caataatatt attagaaaca atcactttga
       61 tcgaaacatt tctaacttct tttattcgtt cattaaccat gcgaactgct agttttccaa
      121 tttcttctgt attcacattc accgtattta atgcaggtgt taaaaattca gcaacttcaa
      181 tattatcaaa actcacaatc gaaatatctt ctggaatatg tagaccattt ttttgaagtg
      241 aacgataaac ccccacagca ataggatcat ttcctaccat gatagcagtc ggcaacttat
      301 ctttagcatg ttttttcaaa agtaaatctg ctgcctgcat tccgtcaagt gtggtccagc
      361 cttctaaata aatttgacta tatttttcta atcctttttg tttcatccac gtctcatagg
      421 ctatttttcg ataatcatca tctgacacgt gtttatttcc ttggctatct aaacgaacac
      481 gtcctcctcc gataaacgca atgtttcggt gtcctttttt atacaatcga tctaaatgct
      541 gattcgttgc ttttgttaaa tcagtataaa ctgcatctac ttcagcttca atactagggt
      601 catcaatcac tacgacattc gaattaattg ctgatacttc ttcaatcaca gaagatagca
      661 cctgcccaat aatcaacaca ccttgacatt ttagcaaatc gtcttgctcc aacggtgttt
      721 ctgacaaacg aataattttc ttcaaattga ctttgctttg ttctgcttcg acttgaatcc
      781 ctctgcgaat cgttcgaaaa tagggatctt ccaactcatc tacttcagaa actgtcgtta
      841 ttaatgcaat atttgttaaa ttttttgcaa cgggactttt tttgacataa tgataaaaat
      901 taaccgattc tgcaatcttt cgtttggttt catcggttac tgaaagtgtc ggatcctcat
      961 ttagcacgcg cgatactgtt gcaatggaca cacccgcccg ttttgcaaca tccctaattc
     1021 ctaccataat agctcctctt ttcatatttg aaagtgttta ctaacatata atcatacatt
     1081 ttgaaaataa gcaaatttcg ttaaaaatag gcactgtaat cataattaaa aacaagaaaa
     1141 aatatccgaa caatttagag taaataatgc taattctagt gaatttgcga cgaggaactg
     1201 ctagatcaca gttcattctc tctatcgttc ggatatttta ttgttaacta tacttatttc
     1261 acagagcttt tttatttgat tttgtgaaca gagtctcgag aaatcaattc tccctctaat
     1321 agtagtactt gattttttat ttcttcttgt ttggccagtc caatcagttg gtgaaaagca
     1381 atcgccccct tgttacctgg atttagtcgt attgttgtca acgcaggatc cattaattct
     1441 gcaaaagtaa tatcatcaaa tccaataatc gataattgtt ccatcactcg tttattctta
     1501 ttttttaaaa attttactaa tttagccgct aaataatctc cagtaacaac aattgcagta
     1561 aattcagcta attcttgata aattcgctca aattcagagt tgttatatat ccaatgcgat
     1621 ttaaaggtta aatgatattt ctttaatgta tcgatataag cttgatgtcg ttcttgaata
     1681 acacttggtt cacctacaat gtagtcaaaa gaaagaaacg ctattttctc atgaccataa
     1741 tgaatcaaat gttccactgc caatgtacta gcttggtaat catttgtatt tacaaataaa
     1801 cgattcattt gttgttcaag ttctcttaat gtctcttttg aaagatgcgt atcaatataa
     1861 acaacaggac catcaaaaac gttatttaat tccaaaaatt cttttacacg tacaccaaca
     1921 acaatgaacc cctcaaaacg ccaatttctc tgaatagttt taacatcttc tgctgaatta
     1981 acattatgca ccaatacaaa ataaccatga ttctttgcct gttcctcaat tccagacaaa
     2041 acttctgcgg taaatggatc agaaaataca aaatcagcat tatcaatagc cgaaaaataa
     2101 agcaatccta ttaaaaatgc aatcgtctta ccgattccct gacagacaga acacattcca
     2161 ctaggatcat taaatgaata agcatttgca tatcctaaac taggtgttgc aaaacatgaa
     2221 aacaacacac gaaataacgc attgatgtta gtaatcgtcc ctaacgttga gcgcgcactg
     2281 ccaccaatct tcttttgatt gaccacaatc gccgtagaaa gattatcaat ctcatcaacc
     2341 tctggacgac tgtatttagg taaaaataaa cgggtaaaac tatcataggt gtcattcagt
     2401 tgacgaccag cttcttgggt aatagtatca aaaacaatcg atgattttcc tgaaccagat
     2461 acgctggtaa aaatactaat tttatttttg ggaattttga gactgacatt tttaagttgt
     2521 ttctcttgcg tggataattt caatatactc gttcatttgg tactcccctt tattttgaaa
     2581 tagttgagaa tgtttcaatg agacacggta tttttggcag atgtttagtt tacctagaaa
     2641 gaggtataaa aatcaagaaa aaaagagagg ctgactcata agtagtttta ttagtcgaaa
     2701 atgatagttt ataaaaatag gaaccgcatc aaatcaacat tctttagaat ggatttgatg
     2761 cgtttttctt tattttttga cttatgagtc aacctcttac tcttcgtcat ggatttgatt
     2821 agctaattat tgctccactt gttcacgaat gaaatttaag tactgcggta aaaataaagg
     2881 aatttcttca aagcgtggac tttgattttc atacgctttt gcatctaggt ttccttcccc
     2941 tgtagcaatt tcaatcaata attgatttgg gtcacgataa taaagtgata caaaaaattc
     3001 acgatctttt attcctgaat ttataaaatt gcgctcttgt aattgtttgt cgatgcgttg
     3061 taaatcagat ttattttcta cgccaaacgc aacatgatgg acgctaccaa tcccttcaat
     3121 ggcaactgga ttctcacgat cttgaataat ctgaacttct tgatagaact gagtgtcttg
     3181 attttctaag attgtcactc gttgatgacc cgaaaagaaa ctgacgtatt ctttgtgcta
     3241 ccagcctacc gacaataact ctcgttcagt cgcatcggaa tattgtacgc gtagttgaat
     3301 ggcatcaatt cctaaaatgg catgctctgt agggatctgg tctgtgacat acgggaaata
     3361 atcatcaata tcttcaaatt cgcgtaaggg aactaaagct aatcgtgttg catctggccc
     3421 ttcaaaacgt aagataggtc gtccagaaaa tgattcgatt ccataatggc aaacgccttg
     3481 tgtttcaaaa tatatttccc aaaaatgcaa tgatgcttcg gaaggaactt ttagtatcgt
     3541 tcgttcaatc gtattggttc caaaaaactg gttgttccct tcttgtactt caaaaaaagt
     3601 cagttcagtt cccacacggt ggtgattatc tgaaaaaaac aggtgataca tggtatgatc
     3661 gtcttgatta attgttttca ttagtaattt taaaccgagt atattatgat aaaagtcaaa
     3721 tgtcggtcga ataaagcgat tcacaataga aatgtgatga atacgtgcgg gcataatatc
     3781 taaaaatgag aatcaaacct ataaaagatg gtgaacaact tttgttttca tcagaagaaa
     3841 ataagcccct ttatttagac tatgtaaatc acaatttgaa aatcatcatt aaagagaata
     3901 acttaaaaca tatcacactt catggttttc gtcataccca ttgtagtctg ctctttgaat
     3961 ctggtgcgtc tgtcaaagaa gtgtatgttc gacttggaca tacagatatt aaaaccacta
     4021 tggatgatat atacacccat atcgcgaaaa agcagacaga ggaaacggca attcgttttg
     4081 cagatttcat gagttctaca gaggaaaagc aaggaagttt gcagaaggtc aaaagtatgg
     4141 tcaaaaacga aaaaagcaga ctatccctat tagatagtct gctaaacgtt gagttatcag
     4201 tatttttctt attccgcagc gtttgcgttt ttgagaccgt attgattctc tttttttaat
     4261 ttcatccttt tccaacctaa aaatgttatt taattactta gtttatataa cacgaaccta
     4321 taccaagtac cagtaacccc agcttttatt ccgcgaactt tatgaaataa aatcgttcta
     4381 gataaaaata gcttttaatc tttgtcttta ctgtttataa aaatttttaa attatacacc
     4441 tagtttaaat ccacgaagtg atgctcctat ttcatcttca gttataccaa tgtacctttt
     4501 tgtaattgct tgcgagctat gattaaaaat aaacattaag gtagcaatgt ctctagtttt
     4561 cttgtagtag tgatacccaa aagtttttct taatgtatgt gttcctatat catctcgtcc
     4621 taatttttct gctatatctg catatatctg atagacacga tcccttgtta tatgtcgacc
     4681 ttgttgccga ctcggaaata accaatcatt ttcttcttta ccttcagtat ataataaaat
     4741 ttcttctctc aaattatcta taaaaatcgg ttttcgctta cctgttttat gttcaacaat
     4801 aataggctta cttttgcctc gtatatctcc aacttttaat cctataatat ctgacatacg
     4861 taacccatta ttgattccaa acaaaaataa aaaactatct ctttccggat tacttgttct
     4921 tcttagccaa aactgaaaat cttgtatctc ttcatttgtc cttaacggtt gtacattata
     4981 gctcactatt gtttcccttc ttaaaaatat tctgagttaa tgattatgta tatattttac
     5041 cataatcatt aacttatat
//
LOCUS       DACDKM010000140         4972 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20834_2016
            SAMN12640370-rid9891663.denovo.140, whole genome shotgun sequence.
ACCESSION   DACDKM010000140 DACDKM010000000
VERSION     DACDKM010000140.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640370
            Sequence Read Archive: SRR10040891
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4972)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4972)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742835.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742835.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:04:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,012
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 29 of 75
            Pseudo Genes (incomplete)         :: 33 of 75
            Pseudo Genes (internal stop)      :: 19 of 75
            Pseudo Genes (multiple problems)  :: 5 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4972
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640370-rid9891663.denovo.140"
                     /isolate="E20834_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(406..780)
                     /locus_tag="IV334_002567"
     CDS             complement(406..780)
                     /locus_tag="IV334_002567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016179839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HAP8944589.1"
                     /translation="MKLNGLQHIGIPTKDFGASKIFYESLGFSLINEEMNVGSKVGFF
                     ELNGTIIEIWEDKTTECRGAIDHLALDTDDIDEVFNSIQDLGYELLDKNIMELPFWEN
                     GIRYFNFYGPNHEIIEISQMKK"
     gene            complement(803..2203)
                     /locus_tag="IV334_002568"
     CDS             complement(803..2203)
                     /locus_tag="IV334_002568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-fructofuranosidase"
                     /protein_id="HAP8944590.1"
                     /translation="MQKIFYKPKDFWFGDCMPFYKDGTFYLFHQRDTRNPKPLTDPFG
                     WSLVTTTDFVKFEEYGEVIHRGGNDDIDQYIYAGSIVQGINEYLALYTGHNRKAKLDG
                     KTSEVLMIASSKDLIHWKKDGEAAKLVPQEGYDKNDWRDPFAIFDQDHGKYVLILGAR
                     KPASKKKPTGRLVYFESEDLKDWNFKGDYWANNEFNMIEMPDIFEIDGTWYLLFSEYC
                     EDKKTKYRVGAGLFSDWKSPVDEAFDGKAYYAARTVGTNETGRFLTGWVATKEGENDQ
                     NNWDWGGALVPHQIIQKTDKTLGVKLPEKIIENFKEITPFSSFKLEKEFGKKERVIVP
                     KIEDYFLFETTLTITSLTKEFALKLFVDSDTQEGYEFRISLINNQLTFGKTPNYPWPQ
                     MFDKGLQRPLFLEIGKEYELKLIVDDTIAVLYIDNVALSTRMYDKVGTALSMTVTEGA
                     IKVDKLDYSINYECGF"
     gene            complement(2207..2617)
                     /locus_tag="IV334_002569"
     CDS             complement(2207..2617)
                     /locus_tag="IV334_002569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAP8944591.1"
                     /translation="MEFIITSHGNIAIGFVDTLKMFFGDNLNCHVVTLGDAGIEEFRE
                     NIEKIVSQVKNQEVMVFADLIGGTPFNEFAKRSEVFQNGFQLLGGMNLGILIEAVNLR
                     LQGKKSDDIIENLRNMNTIACLADMQRHTNEEDE"
     gene            complement(2635..3126)
                     /locus_tag="IV334_002570"
     CDS             complement(2635..3126)
                     /locus_tag="IV334_002570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAP8944592.1"
                     /translation="MDEKMKGIIHVRIDDRMIHGQVATQWTNQLSANRIMVINNEVAN
                     DDVKKAVVRLAAPPNVRTSIITREVAVKNILSGKYEGQKVLIVAVSPIDVKFLIDNGL
                     PITSVNVGNLSRRNGTERIRPTINITEEEREAFKELISDGVEVTVIQTPRDSKEFLKD
                     FIY"
     gene            complement(3136..3927)
                     /locus_tag="IV334_002571"
     CDS             complement(3136..3927)
                     /locus_tag="IV334_002571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HAP8944593.1"
                     /translation="MNKKLTKIDKQVIFRWILNGATSLNYEKMEGLGYAYSMLPFIKD
                     TYKDDPEGMKQSVLAHLQFFNTTPYTAPYIIGMNVGIEKEEKNKSLEAVSALKTGLMG
                     PLAGIGDSVFVVIPWTIFGAIAANMALEGSFVGIILWILASILLRITAYPLYLAGKES
                     GASLLTQIETKLKSITDAVSILGLMVVGALIATVVKVNVGFEFKQGDLTMTGNEIFDQ
                     VMPGLLPACVVAIVYYLLGRKVKPVYVILLILVVSIALRALNILK"
     gene            complement(3920..4693)
                     /locus_tag="IV334_002572"
     CDS             complement(3920..4693)
                     /locus_tag="IV334_002572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAP8944594.1"
                     /translation="MAFWQTFLVVLYGFFINWDKEGPKIGTSQPVVAGFLTGLIMGDI
                     QTGLYIGGTLQLMTLGITSFGGASVPDYQTAALIGTYLAISTNQDASIGITLAIPVAM
                     LIVQLDVLKWSTNIFFQQKAEKFAQKEDYQKLEWMQYLASINTSFVSGIPVLLTVILG
                     PTLVGSAINYIPEWVTGGLKVAGGLLPAVGIGMLLKYLPTKDYFAYLLVGFVLAVYFQ
                     IPILGISLLGAAIALIVYKNRSNNTQVAPAATGGFDEDE"
BASE COUNT         1587 a         1008 c          655 g         1722 t
ORIGIN      
        1 ctaggagtga gcatttttcg aaaaatacta aaaatttttc aaaataaaaa aaggcataca
       61 gacaataaac tgtctatacg cctcacagac tctattctat acatctaatt ttggctgtcc
      121 tgcaaaaaat atgtgttagc ctccatcgaa agatcaaact ttggaaaaca tcattataac
      181 tttgcttggt aagagctttg ctaaaacatg atatgacttg tatacaaaat gtggagtgta
      241 ttccaataaa taggaaggat tacaaaattt gaaaccaaat aagtaaaaaa tactgtcact
      301 ttgaattaat ttgaaatagt aaattctcat taaactattg aaatgctgat accaaaaaat
      361 gaagtacttt tcgttcatac caacttagag cacttcgtta tctatttatt tcttcatttg
      421 gcttatctct ataatctcat gatttggtcc gtagaaatta aagtaacgga ttccattttc
      481 ccagaacggt aattccataa tatttttatc taataattca tatcctaaat cttgaattga
      541 attgaatacc tcatctatgt catccgtatc taatgctaga tgatcaatgg ctcctctaca
      601 ctcagtagtt ttatcttccc atatttctat tatcgttcca ttcaactcga aaaagccgac
      661 tttacttccc acattcattt cttcattaat cagactaaac cctaaggatt cataaaagat
      721 ttttgaagct ccaaaatctt ttgttggaat tccaatatgt tgtaaaccat ttaatttcac
      781 ttacatcatc ccttcaattg ttttaaaatc cacattcata atttattgaa tagtctagtt
      841 tatctacctt gattgctccc tccgtaacag tcatacttaa tgctgttccg actttatcat
      901 acattctagt acttaaagca acattatcaa tatataaaac agctatcgta tcatcaacaa
      961 ttaactttag ttcatattct ttgcctattt ccaaaaatag tggtcgttgc agccccttat
     1021 caaacatttg aggccaagga tagttcggag tttttccgaa agtaagctga ttattaatca
     1081 atgaaatacg aaattcgtat ccttcttgag tatcactatc tacaaataat tttaaagcaa
     1141 actcctttgt taacgatgta atagtcaaag ttgtttcaaa tagaaaataa tcttcgattt
     1201 taggaacaat tactctttct ttttttccaa attctttctc taacttaaaa cttgaaaatg
     1261 gagtgatttc tttaaaattc tcaattattt tttcaggcag tttaacacca agagttttgt
     1321 ctgtcttttg aataatttga tgtggaacaa gtgcaccgcc ccaatcccag ttattttgat
     1381 cgttctcccc ttcttttgtt gccacccagc cagttagaaa acggccagtt tcattagttc
     1441 cgactgttct tgctgcataa taggcttttc cgtcgaaagc ttcgtctact ggtgatttcc
     1501 aatctgaaaa taatcctgct ccgactcgat atttagtctt tttatcttca caatactctg
     1561 aaaaaagtaa ataccacgtt ccatcaattt caaatatatc aggcatttca atcatgttaa
     1621 attcattatt agcccagtag tctcctttga aattccaatc ttttaaatct tcggattcaa
     1681 aatagaccaa tctccccgtc ggctttttct tacttgctgg ttttctagct cctagtatca
     1741 aaacgtattt tccatgatct tgatcaaaaa ttgcaaaagg atcgcgccaa tcattcttat
     1801 catatccttc ttgaggaaca agttttgctg cttcgccatc ctttttccaa tgaattaaat
     1861 ctttagaact tgcaatcatt aacacttctg aagtttttcc atccaactta gctttgcgat
     1921 tatgtccagt atacaaagct aaatactcat tgattccttg gacaatactt ccagcgtaaa
     1981 tgtattgatc aatgtcatcg ttgccaccac gatgaattac ttccccgtat tcttcaaatt
     2041 tcacaaaatc agtggttgta actagtgacc acccaaaagg atcagttaaa ggtttaggat
     2101 ttctcgtatc tcgttgatga aatagataaa aagttccatc tttataaaaa ggcatgcaat
     2161 ccccaaacca aaaatcttta ggcttgtaaa aaattttttg catttatcac tcatcctctt
     2221 cgttagtatg tctttgcata tctgcaagac acgcaattgt attcatattt cttagatttt
     2281 cgatgatatc atcgcttttt tttccttgta atctcaaatt gacagcctca atcaaaattc
     2341 ctaaattcat gccaccaaga agttgaaacc cattttgaaa tacttcactt cttttagcaa
     2401 attcgttgaa aggtgttcca ccaatcaagt cagcaaatac cataacttct tgatttttta
     2461 cttgagaaac tattttttca atgttttcac ggaattcttc aatgcctgca tctccaagtg
     2521 ttaccacatg acaatttaaa ttatcgccaa aaaacatttt taatgtatct acaaatccaa
     2581 tggcaatatt tccatgagat gtaattataa actccatcca ctcatcctct gttcttaata
     2641 aataaaatcc tttaaaaatt cttttgaatc tctaggagtt tgtatcactg tcacttcaac
     2701 tccatcacta attagttctt taaaagcctc tctctcttct tcagtaatat ttatcgtcgg
     2761 acggatgcgt tctgttccat tccttcttga taaatttcct acgtttacac ttgttattgg
     2821 taaaccgtta tcaatcaaaa atttaacatc gattggagaa actgcaacaa taagaacttt
     2881 ttgtccttca tatttaccag aaagaatatt tttaactgct acttcccttg taattattga
     2941 agttctaaca ttaggtggtg cagcgaggcg aactacagct ttttttacgt catcattggc
     3001 tacttcatta ttaataacca taattctgtt agcactcaat tgatttgtcc attgtgttgc
     3061 tacttgtcca tgaatcattc gatcatcaat acgaacatgt ataatacctt tcattttttc
     3121 atccatattc aacacctact tcaaaatatt taacgctctt aacgcaatag aaactactaa
     3181 gattaataga attacataaa ctggtttaac ctttcgtcct aacaaataat acacaattgc
     3241 tactacacat gcaggtaata aaccaggcat aacttgatca aagatttcat ttccggtcat
     3301 tgttaagtcg ccttgtttaa attcaaagcc aacattcact tttacaactg tggcaatgag
     3361 tgcaccgacc accattaacc ctaaaattga tactgcatca gtaatagact ttaattttgt
     3421 ttcaatttga gttagtaggc tcgcacccga ttctttccca gcaaggtata aaggataagc
     3481 agtaatcctt aataaaattg atgccagtat ccacaaaata attccaacaa aactaccttc
     3541 caatgccata ttagctgcaa ttgctccaaa aatagtccat ggaataacta caaatacact
     3601 atctccaatt cccgctaaag gacccattag acctgttttc agagcactta ctgcttccaa
     3661 agacttattt ttttcttctt tttcaattcc aacattcata ccaataatgt aaggcgctgt
     3721 atacggcgta gtgttaaaaa attgtaagtg agctaaaacg gattgtttca ttccttcagg
     3781 atcatcttta tatgtgtcct tgataaaagg aagcatagaa tacgcataac ccaacccttc
     3841 cattttttca taatttaatg aagttgcacc attgagaatc caccggaaaa ttacttgttt
     3901 gtcgattttt gtcagtttct tattcatctt cgtcaaaacc tcctgttgca gctggtgcta
     3961 cttgagtatt atttgatcta tttttatata cgattagtgc gattgcagcg ccaagtaacg
     4021 aaatccctaa aattggtatt tggaaataaa ctgccaaaac gaaaccaact aacaaatagg
     4081 caaagtaatc ttttgtaggt aaatatttaa gtagcattcc tatcccaaca gcaggtaata
     4141 gtccccctgc tacctttaaa ccaccggtaa cccattcagg aatataattg attgcacttc
     4201 ccactaacgt tgggcctaat atcacagtta acaaaacagg aattccagat acaaagcttg
     4261 tattaattga tgccaaatat tgcatccact ctaatttttg ataatcttct ttttgtgcga
     4321 atttttctgc cttttgttga aaaaaaatgt ttgttgacca ctttagaaca tctagttgta
     4381 caattaacat tgctaccggt attgctaaag ttattcctat agaagcatcc tggttagttg
     4441 agattgctaa ataagtaccg atcaatgctg ctgtttgata atccggtact gatgcaccac
     4501 caaaactagt aatacctagt gtcattaatt gcaaagtccc accgatataa agtccagttt
     4561 gaatatctcc cataataagt ccggtcaaga aacctgcaac gacaggttgg gaggttccga
     4621 ttttgggccc ttccttatcc cagttaatga aaaatccata caacacaact aaaaatgttt
     4681 gccaaaacgc cattttgttc tctcctttct tcttatactt ttatataagc aattactgtg
     4741 ccaactaaca ttatccaata aagcgggaaa atacagtaaa tattaattaa atgagcgtct
     4801 aaatatactt tggacaccct ttcatttttt tagacgctag tatgctattt agaaaatagt
     4861 ctgctcaaaa attgtataga ttaactcata ctctcctttg ggtatttcaa tacagtaaat
     4921 atctttgatt tctgaaaaaa aatcttttga tacatttaaa aaagtatcaa at
//
LOCUS       DACDKM010000141         4947 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20834_2016
            SAMN12640370-rid9891663.denovo.141, whole genome shotgun sequence.
ACCESSION   DACDKM010000141 DACDKM010000000
VERSION     DACDKM010000141.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640370
            Sequence Read Archive: SRR10040891
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4947)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4947)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742835.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742835.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:04:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,012
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 29 of 75
            Pseudo Genes (incomplete)         :: 33 of 75
            Pseudo Genes (internal stop)      :: 19 of 75
            Pseudo Genes (multiple problems)  :: 5 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4947
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640370-rid9891663.denovo.141"
                     /isolate="E20834_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(62..280)
                     /locus_tag="IV334_002573"
                     /pseudo
     CDS             complement(62..280)
                     /locus_tag="IV334_002573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391850.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(546..740)
                     /locus_tag="IV334_002574"
     CDS             complement(546..740)
                     /locus_tag="IV334_002574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8944595.1"
                     /translation="MMGFFLRFYIIELTGRLTVFFGNFAIKPFAEVTAHYICKWLDCK
                     NKIKKVFHYISSSPCQIRPG"
     gene            2092..3132
                     /locus_tag="IV334_002575"
     CDS             2092..3132
                     /locus_tag="IV334_002575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein RepA"
                     /protein_id="HAP8944596.1"
                     /translation="MTDFKFFKADRVYNELFYQFPKVFIVSDEYKKMKDSTKIAYMLL
                     KARLEIAISKRQIDEEGNVYFTYTTNELCRVLNCQKQKAIAIKKELESFGLLLQKQMG
                     FNKQLGKNNPNRLYLAELKVSENDIYLLEKFDRENRENVDKSEGMKIIPTLDEKSDAE
                     SLGAQEGMKIIPCQNVDKSEGMKIIPELNNNILDTNRHNIDTEKDRLQDQLLLDNFET
                     IMTNDSIATFVPERVLNLIKTFSSSYSEAQKTVQTIHNAKKKAEIESGISIVFEELDS
                     YYVNAEQELYTTLLKAYQKLKTEKVENIQNLIFVYVKNWFIEKPIAAKVSSEKRLNYE
                     SSPSTITKDWLE"
     gene            3956..4288
                     /locus_tag="IV334_002576"
     CDS             3956..4288
                     /locus_tag="IV334_002576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraE"
                     /protein_id="HAP8944597.1"
                     /translation="MSYTGILSLKDICHYGKRCTATEKITKKLSTGQSKTVVQCKKYI
                     IQKDKVSEEMIYYIGKQKQIILKDPIPLKELYPTIKHVYDQNGVLIGRRKNGVLRCTA
                     KGMGRLIG"
BASE COUNT         1803 a          690 c          807 g         1647 t
ORIGIN      
        1 taggtatttt tattatatat agatttaaca tatgtcggtc ggttgattga tatagctaaa
       61 gttataactc aaccgaagcc gattcatcta cacgaataaa gttgcgagtg ttctcggcta
      121 attcataaaa attgttatgt gttccatata gcaatactgg aataacttta ttttcttgaa
      181 tctcataagc aactctatac acggtgaccg catacttaaa tccattagac caattacctg
      241 ataaacctcc agttttttgc tcttacttaa atggatcaat ggcaatttca tcacaaacct
      301 atagaggatg agtgacttct gcctaattac gttaaaaaat attgaaagta actctatgta
      361 gcaaaaaata tctagaattt catttcctca aaactgatcg atattgtatc taaaaatata
      421 tactacctaa aataggtagt atatattaaa atatgtgctt ttgttagaaa aatcaataca
      481 taaattatca tatctgtata tgtcatttag gtatttcagt tatgttataa tagttttgct
      541 gtctgctacc ccggcctaat ctggcaaggg gaggaggaga tataatgaaa tacctttttg
      601 attttgtttt tgcaatcaag ccacttgcag atgtagtgtg cagttacctc tgcaaatggc
      661 ttgattgcaa aattgccaaa aaagacagta agcctacccg ttaattctat aatatagaaa
      721 cgtaaaaaaa accccatcag cctgcacgct gatggggttt ggtgatataa tgaaatacct
      781 ctttgttact cataattgta ccataattag tcacgaatgc aaccaataga aaaaactact
      841 tagaacggta gatcatcgct aataacaatg cattgtccac caaatggatt ttgtgaagca
      901 ttaaatacat tgttattaat tgtcgctgct tttaactagg cctctcccct tctattataa
      961 aagaagatat atatcatttc ttaattacct actaccttaa accaaaattt aagtactacc
     1021 taatttaagt attacttaaa caatctaaaa aatagttatc ccaattgtta tgcttttata
     1081 atacgattat ttgtaacaaa atgaatatta tataattaaa cactaccata tttaggtagt
     1141 gtttaattat tagcaaaaca aaaaatattt attctattct cttaacttca actaagttat
     1201 gctgcacacc tttatataaa tcatatgcaa ctcattagct agacaaacta caagcatgta
     1261 gttttatctg ttaattatgt actacctaaa taaagtagtt tataattagc aagtatatat
     1321 atttaaaaat ttaagattaa tttttttatg acttgtacaa atttatctga tattctctta
     1381 ttaagaacta cctaatttgg gtagttctta ttttttacat tttaagtagt atttaattgc
     1441 aatttggtag tattttttta ttatcttatt cttagaacgg tagtacttaa tataaatata
     1501 ggtagtatct aatttaagta ttaatggtag tatctaaatt aacaggtagt atgtaactac
     1561 tttaaagtaa tttttttcag attaacacag tagtatctta ttaaataagt taattttaat
     1621 ggtagtacat aattattata tatatctgac attggtagta cttattgtat tttttagtag
     1681 tatataatct attgttttct agcctaaagc cttgtcttat aagaataata taatatatac
     1741 tactgaaata aggtagtata tattactagt aatggtagta tgttttatat gcataaggta
     1801 gttcttaatt tattttttgt tttgacactg gtagtactta attatcttta attttaaaac
     1861 taaattttat tcatacgttt ttctggtagt atttaattat aaataatatt tatttaaagt
     1921 agttcttaat gactttaaag gtagtacata attcatttta tttctgattt taggtagtat
     1981 ttaattaaaa aacagtttta atgcgtttgc attttttatt tatctttgct attttcgaaa
     2041 agagtaggta attttacata aataaaacaa cgtgaaagta gaggatctac aatgactgat
     2101 tttaaatttt ttaaggcgga tagagtttac aacgaattat tttatcaatt tccaaaagtc
     2161 tttattgttt ctgacgaata caaaaaaatg aaagattcaa ctaagattgc ctatatgctt
     2221 ttaaaagcaa gattagagat cgcaatcagc aaacggcaaa tcgatgaaga aggtaatgtt
     2281 tattttactt atacgacaaa tgaactatgt agagtattaa actgccaaaa acaaaaagcg
     2341 atagcaatca aaaaagagtt ggaatccttt ggtttattat tacaaaagca gatgggattt
     2401 aacaaacagt tagggaaaaa taatcctaat agactatatc tagcagaatt aaaagtctca
     2461 gaaaatgata tctacttact cgaaaaattt gatagagaga atagggaaaa cgttgataaa
     2521 tcagagggta tgaaaatcat acccaccctc gacgaaaaat cagacgctga atcccttggg
     2581 gctcaagagg gtatgaaaat cataccgtgc caaaacgttg ataaatcaga gggtatgaaa
     2641 atcataccag aacttaataa taatatatta gacactaata gacacaatat agacactgaa
     2701 aaagaccgcc tacaagatca attgttgtta gacaattttg agacaattat gacaaacgac
     2761 agcattgcta cgtttgtccc tgaacgagta ttaaatttga taaaaacatt ttcttcaagt
     2821 tacagtgaag ctcaaaaaac cgtccagact attcataatg caaagaaaaa agctgaaata
     2881 gaaagtggta tttcgatagt ttttgaagaa ctcgatagtt attatgtcaa tgcagaacaa
     2941 gaattataca cgacactgtt aaaagcctat caaaaattaa aaaccgaaaa agtcgaaaat
     3001 atccagaacc tgatttttgt ctatgtaaaa aattggttta tcgaaaaacc aatagctgct
     3061 aaagtatcaa gtgaaaaacg tttgaattat gaaagctccc caagcactat tacgaaagac
     3121 tggttagagt gaaactttac tgacaaagtg attgttgctt catggagtaa cattaaaatt
     3181 aaaaattttt tattattcta attatattac gaatattaca aaataatagc tattttaata
     3241 gaatttataa gttaaaatat tatgataaaa atcacaaacg tagcctttta cgtcataagt
     3301 gttaaaagaa ataattaatt tcaaatatag aaaagggaac gtatgtttgc atactccttt
     3361 tttttgagtt ataataatac gcgaagagag ataatttcat tatttaaaca agctaaaaat
     3421 cttgatatta cttcttttca ggcaatacga atattattcg cttacatttt ttgtataaat
     3481 ttaattgtct gctatgcttg tgatgaattg attttcgttc tgcacttttg gatgataaat
     3541 ttggacaatt gaaaatacaa aaaaaagaaa ggcggtatga actcggaagg ctggtaacct
     3601 tcgtcagcgt tcatacacgg cattaagatt tggcccttaa tgcgttaacc tttcagtgtt
     3661 acggtattac ccgcaatgga tttagtataa cagatattca aaagtgatac cacttttttt
     3721 ggctgtgtta aggttaaaaa tacgtgatat tacgatgaat ctatgcctaa gcacaatgtt
     3781 ataaaaatgt ccgtgtggct ctttgccgta tgacgaacgc tgaaattaga gggaacgcat
     3841 gtttggatgc gttccctctt ttttgtttgt catgaacgaa aatcaattct ctatttttga
     3901 aaaataacaa gaaaaacttg ttaaaaatat ttaaagaaat tgggtggttt attttatgtc
     3961 atatacagga attttgagtc tcaaagatat ttgtcactac ggtaaacggt gtaccgctac
     4021 agagaaaatc acgaagaaac tatcaactgg ccaaagtaaa accgttgtgc agtgtaagaa
     4081 atatataatc cagaaagaca aagtctcaga ggaaatgatt tattacattg ggaaacaaaa
     4141 gcaaataatt ttaaaagatc ctatcccctt aaaagagcta tatccaacaa tcaaacatgt
     4201 ttatgaccaa aacggtgtac tgattggtcg aagaaaaaat ggagtcttgc gttgtaccgc
     4261 aaaagggatg gggcgtttga tcggctgaat tgatcattct cataacttgt attttatcgt
     4321 ttttcgcttg cttgaaaata ttgtcgagac ttctaaagga acttagagtc actagaaaaa
     4381 taaggagtag acttgatcta tcaacaattc ccttcgggga gtgacggggt aggttttaac
     4441 gattggtaac gtaaaaaatt ggtcaatcga gtaaatgcgc ttgtttggat agtggtgaaa
     4501 tgaatttgtg taaataataa gcgcagagta aacaagaggg aatttagaag ttagacaagt
     4561 tagaatagcg tctagattaa tcatgagcag ctactaaggg atctagactt tgacgcaaac
     4621 tcatggaata aggagtaggt cttctcgtaa tggggaggac gcacaagaaa ttgtgtcgtg
     4681 tgctgcagta catgtagata cttgcgagga cgttgattat tggatcaatc gagcatttac
     4741 cgctgtctac tagggtttct cgtttcggac gccataaacg agtcgaaatt ttttatgtat
     4801 ttttaagtaa acgaaaaacg ataaaatatg aacaagtcct gaataaagtg aaaaaattga
     4861 tataaaacat agagaaaatg aaatttacgt aatggaggaa tgacaaatga aaaattatga
     4921 tccgaatata cgattgggca cacatac
//
LOCUS       DACDKM010000142         4935 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20834_2016
            SAMN12640370-rid9891663.denovo.142, whole genome shotgun sequence.
ACCESSION   DACDKM010000142 DACDKM010000000
VERSION     DACDKM010000142.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640370
            Sequence Read Archive: SRR10040891
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4935)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4935)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742835.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742835.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:04:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,012
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 29 of 75
            Pseudo Genes (incomplete)         :: 33 of 75
            Pseudo Genes (internal stop)      :: 19 of 75
            Pseudo Genes (multiple problems)  :: 5 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4935
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640370-rid9891663.denovo.142"
                     /isolate="E20834_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(117..1889)
                     /locus_tag="IV334_002577"
     CDS             complement(117..1889)
                     /locus_tag="IV334_002577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002841847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP8944598.1"
                     /translation="MRMQMIGGLTMKSFYKNKFALSDQGAEALTKASISSFFVYCINM
                     VPAFIIMMLIDELVLENTKPRWLYFAVSFVTLLFMYWLLDREYENLYNSTYKESAHLR
                     VQIADDLSNLPLSYFSKHNLSDLSQTIMSDVEGIEHAMSHAIPKAGGMALFFPFISVM
                     LLVGNVKMGLAVILPTLFSFVLILLSKKSQTKANTKYYDTLRENSEEFQETIELQQEI
                     NSFNLSKKVQDRLFKKMEESERIHLKVELSTFSVMALSSIFSYVSLAVVILVGVHLLL
                     TGEVTILYVVGYLLAAIKIKDSFDSMKEAVLEIFYLAPKIQRIRAMKETSIQEGSDSP
                     LKSFDIELRDVSFSYDNNTPILDHISFTAKQGEVTALVGASGSGKTSILKLVSRLYDY
                     DEGCILIDGYDIKRVSPASLFSKIAIVFQEVTLFNTSILENIRIGNSQASDEEVKKAA
                     RLANCEDFIEKLPDGYHTLVGENGSSLSGGERQRLSIARAFLKNAPILILDEITASLD
                     AENEKRIQESLNRLIQDKTVLIISHRLKSIEKVNKIVVMDQGKLVDQGTHNELYRRSE
                     IYKNLIKKTKLSEKFVYEKEAQSR"
     gene            complement(1843..3597)
                     /locus_tag="IV334_002578"
     CDS             complement(1843..3597)
                     /locus_tag="IV334_002578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016180774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP8944599.1"
                     /translation="MKVYKKLFSYVPKMKYYGWLATLFSDFSVVLTVYGYYSIYKFLQ
                     SLIIVGDEYLAKSYAVQTVAWLTLGALFYIFSGLFSHILGFRLETNLRKKGISGLTEA
                     SFRFFDLNSSGYIRKTIDDNAAKTHMAIAHLIPDSAQAIVTPVLSVALGFFINFKIGL
                     VLIAMLVIGVFLLKKMTENTNFIQKYQESLDVLSSETVEYIRGIQVIKIFGAKVTSFK
                     ALNKAINDYAKFAYKYSLSGKTPFVLFQWLFFGIVCILIVPITFFITSLTTPKILAVD
                     LLMLFFLSGIIFVSFMRIMYVSMHLFEARYAVETLDSLYLEMKQDQLKYGNETQIKNC
                     DIAFENVSFSYKEECVLENFNLVLEQKRIYALVGKSGSGKSTIAKLISGFYNVDKGQI
                     KIGGKPLNHYSKDTIINTIAFVFQDTKLFNQSIYDNVSIANPQAPASKVFDALRLAGC
                     ESILEKFPEREHTLIGSKGVYLSGGEKQRIAIARAILKDAKIVIMDEASASIDPDNEY
                     ELQKAFQHLMKEKTVIMIAHRLSSIKSVDEILVLSEGKIVERGNHNELMAKHQVYKQL
                     VTNYENANDWRLDNEKFL"
     gene            complement(3707..4276)
                     /locus_tag="IV334_002579"
     CDS             complement(3707..4276)
                     /locus_tag="IV334_002579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MptD family putative ECF transporter S
                     component"
                     /protein_id="HAP8944600.1"
                     /translation="MKTKDYIFIGLATVISLVIYFFSIMVSSIGGAFGHSISPGIFGL
                     LSGIIFVYISYNYPRKGIFTIYTIVLLLFFTLMGGAYLPWFISSISMAILADIILSVF
                     GYDRAIPQVASWALMQLGSAAGQWIPIWFFTDRFRQDWIDKGQSAATMDAMIHYAVGI
                     WGIISVLVVASLSMIGVLIGRKVLKKYKK"
     gene            complement(4294..>4935)
                     /locus_tag="IV334_002580"
     CDS             complement(4294..>4935)
                     /locus_tag="IV334_002580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAP8944601.1"
                     /translation="KVEEITYENILKDINFDVKVGDVIGLVGKNGVGKTTLLRILMKI
                     IRPTKGKIIENKKNLSSPFLVMQEMDYQFFTESVRSELALGNEIVSKDQQEKILKKME
                     LYKMKDQLPFDLSGGEKQRLLVSIASLSKTNLFLFDEPTSGLDYINMERIADLIKDLK
                     EKGAVVIATHDPEFLYKTCNGIIYLKNGGIKKDIKLRVEDSTLIEQIFNDITK"
BASE COUNT         1642 a          932 c          668 g         1693 t
ORIGIN      
        1 atcatactga ttctttcgtg tattcaaaat tagatgctgt ttaataaaat tattcttttt
       61 gaaaaagaat tataactata tcccaaaaat tgtaggttaa tttaaagcct atttcatcat
      121 cgtgattgag cttctttttc atagacgaat ttctcagata gtttagtttt ctttatgaga
      181 tttttgtata tttctgacct tcgatacaac tcattatgag taccttgatc aactagtttt
      241 ccttgatcca tcactacaat tttatttacc ttttcaatag acttcaagcg atgcgatata
      301 attagtactg tcttatcttg tattaaacga tttaaacttt cctgaatacg tttttcattt
      361 tccgcatcta aactggcagt aatttcatct aaaattaaaa ttggcgcatt tttcaaaaaa
      421 gcccgtgcta tggataggcg ttgtcgctct cctccagaaa gtgaacttcc gttttctccg
      481 accaatgtat gataaccgtc aggtagtttt tcaataaagt cttcgcagtt agctaaacga
      541 gctgcttttt tcacttcctc atctgacgct tgactatttc caatacgtat attttccaaa
      601 atacttgtat taaacaaagt tacctcttga aaaacaatag cgattttgct aaacaaagaa
      661 gctggagata cacgtttaat atcataccca tcaattaaaa tacagccttc atcataatca
      721 tacagtctag acactaattt caagatacta gttttcccgg aaccactagc acctactaac
      781 gcagtcactt ctccttgttt agccgtaaaa gagatatgat ctaaaattgg ggtattgtta
      841 tcatatgaga aagagacatc tcgcaactca atgtcaaaag atttcagagg actatctgat
      901 ccctcttgga tactggtttc cttcatagct ctaattcgct gtattttagg agctaaataa
      961 aatatttcaa gcactgcttc tttcatagaa tcgaaggaat cctttatttt tattgcggct
     1021 agtaagtaac caacgacgta gagtatagtc acttcacccg ttaacagtaa gtgaacacct
     1081 actagaatga ctactgctaa actaacatat gagaaaatag aggataaggc catgactgaa
     1141 aaagtactta attctacctt taaatgaatc ctttcactct cttccatttt tttgaaaagt
     1201 ctgtcttgaa cttttttaga tagattaaag ctattaatct cttgctgcaa ttctatagtt
     1261 tcttgaaatt cttccgagtt ttctctcaaa gtatcgtaat atttagtatt ggctttcgtt
     1321 tgggatttct ttgataacaa gattaagaca aaactaaata acgttggcaa aataacagct
     1381 aatcccattt tgacattacc aaccaaaagc atcactgaaa taaaagggaa aaacagagcc
     1441 ataccgccgg ctttaggtat tgcatgactc atcgcatgct caataccttc aacgtcagac
     1501 atgatagttt gagataaatc tgataaatta tgttttgaaa aataggataa tggtaaattt
     1561 gacaaatcgt ctgcaatttg cactcttaaa tgcgcacttt ctttataagt actattatat
     1621 aagttttcat actccctatc taacaaccaa tacatgaaaa gcaaagtaac aaacgatacg
     1681 gcaaaataaa gccaacgggg ctttgtattt tctaaaacta attcgtctat cagcatcatt
     1741 ataatgaatg ctggtaccat attaatgcag tatacaaaaa aactagaaat agaagcttta
     1801 gttaaagctt ctgcgccctg atcacttaaa gcaaatttgt ttttataaaa acttttcatt
     1861 gtcaagcctc caatcatttg cattctcata attagtgacc aattgtttat acacttgatg
     1921 tttagccatt aattcattat gatttccacg ttctactatt ttcccttctg acaagacaag
     1981 aatttcatct acagatttaa tgctggataa tcgatgagct atcataataa ctgttttttc
     2041 tttcatcaga tgttgaaaag ccttttgtag ttcatactca ttatctggat caatagatgc
     2101 actggcttca tccataataa caatctttgc atcttttaaa attgctctgg caatagctat
     2161 acgttgtttt tctcctcctg acagataaac tcctttacta ccaattaagg tatgctctct
     2221 ttcgggaaat ttttctaaaa tcgattcgca accggctaat cttagcgcgt caaatacttt
     2281 acttgctggc gcttgaggat ttgctataga aacgttatcg taaatactct gattgaatag
     2341 tttcgtatct tgaaagacaa aagcaattgt gttaataatt gtgtcttttg aatagtgatt
     2401 taaaggctta ccaccaattt taatctgtcc cttatccaca ttgtagaaac ctgaaattag
     2461 tttagctatc gtactctttc cgcttcctga ttttcctact aatgcataaa ttcttttttg
     2521 ttctaaaact aagttgaaat tttctaaaac acactcttct ttgtatgaaa agctgacatt
     2581 ttcaaatgca atgtcacaat ttttaatctg tgtctcgttc ccatatttta gttggtcttg
     2641 cttcatttct aaataaaggg agtctagtgt ttcgactgca tatcttgctt caaacaagtg
     2701 catacttaca tacattattc tcatgaaaga aacaaaaata attccactta aaaaaaacaa
     2761 catcaacaag tcaacagcta atatcttggg agtggtaaga gaagttataa aaaaggttat
     2821 aggaactatc aaaatgcaaa ctatgccaaa aaatagccat tgaaaaagaa caaaaggtgt
     2881 tttcccactc aaagaatact tatatgcaaa cttagcataa tcgttaattg ctttattcaa
     2941 ggctttaaat gatgttactt tagcaccaaa tatctttata acttgaattc cgcggatata
     3001 ttctaccgtt tcagagctta acacatctaa cgattcttga tatttttgaa taaaatttgt
     3061 attctctgtc attttcttta acaagaatac accaattact aacatggcga ttagaaccaa
     3121 tccaatttta aagttaatga aaaagcctaa ggcaactgat aaaacaggtg ttactatggc
     3181 ttgagcacta tctggaatca agtgagcaat tgccatatgt gttttagccg cgttgtcatc
     3241 aattgtcttt cgaatatatc ccgaagaatt taaatcaaaa aaacgaaaac ttgcttctgt
     3301 taaaccgcta attccctttt ttctcaaatt agtctctaag cgaaacccaa ggatatgaga
     3361 aaataagcca gaaaaaatat aaaataaagc tcctaaagtt aaccaagcaa cagtttgaac
     3421 cgcataactc ttagctaaat attcatctcc tacaataatt agagactgaa gaaatttgta
     3481 aatactgtaa tatccataga ctgttaatac gacagaaaaa tcagaaaata aagtggctaa
     3541 ccatccgtag tatttcattt tagggacata actaaataat tttttgtata ctttcatttt
     3601 ttcctccccc gcattcaatt tcttctctta ttcgtttaca actgttaatg ctttccgtaa
     3661 tagcgtaaat tagagatgta caaacagata aaaggtaaac tacgttctat tttttgtatt
     3721 tttttaagac ttttcttcca atcaatacac caatcataga taaacttgct acaactaaca
     3781 ctgaaatgat tccccatata cctaccgcat agtggatcat tgcatccatt gttgcagcag
     3841 attgtccttt atctatccaa tcttgacgaa aacgatctgt aaagaaccat ataggaatcc
     3901 actgtccggc agcactgcct aattgcatta gagcccaaga cgcaacttga ggaattgctc
     3961 gatcatatcc aaatacagat agaataatat cagctaagat cgccatacta atcgaactga
     4021 taaaccaagg taagtacgcc ccgcccatta acgtaaaaaa taacaataaa acaattgtgt
     4081 aaatagtaaa aattcctttt ctagggtagt tatagctaat ataaacaaat atgattccag
     4141 ataataatcc aaaaatcccg ggggatatgc tgtgaccaaa cgccccacca atactactaa
     4201 ccattattga aaaaaagtaa attactaggg aaataactgt agctaaacca ataaaaatat
     4261 aatcttttgt tttcatatat attctcctca attctatttc gttatgtcat taaaaatctg
     4321 ttctatcaaa gttgaatctt ctactctaag ttttatatct ttttttattc caccattttt
     4381 taaataaata attccattgc atgttttata aagaaattcc ggatcatgag tagctataac
     4441 tactgcaccc ttttctttta agtctttaat aaggtcagca atcctttcca tattaatata
     4501 atccaaccca cttgtaggtt catcaaatag aaataaatta gtttttgaca atgaagctat
     4561 agaaaccaat aatctttgtt tttctccacc ggataaatca aacggtaatt gatctttcat
     4621 tttataaagt tccatttttt ttaaaatttt ttcttgctga tctttactaa ctatttcatt
     4681 ccccagggct aactcgctcc tcactgattc tgtgaaaaat tggtaatcca tttcttgcat
     4741 tactaaaaac ggcgatgaca aatttttttt attttcgatt atctttcctt tggtagggcg
     4801 aataatcttc atgagtattc ttagtaaggt tgttttcccc acaccgtttt tccctaccaa
     4861 accaataaca tctccaactt tgacatcaaa atttatgtct tttagaatat tttcataagt
     4921 aatttcttca acttt
//
LOCUS       DACDKM010000143         4800 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20834_2016
            SAMN12640370-rid9891663.denovo.143, whole genome shotgun sequence.
ACCESSION   DACDKM010000143 DACDKM010000000
VERSION     DACDKM010000143.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640370
            Sequence Read Archive: SRR10040891
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4800)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4800)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742835.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742835.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:04:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,012
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 29 of 75
            Pseudo Genes (incomplete)         :: 33 of 75
            Pseudo Genes (internal stop)      :: 19 of 75
            Pseudo Genes (multiple problems)  :: 5 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4800
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640370-rid9891663.denovo.143"
                     /isolate="E20834_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..479
                     /locus_tag="IV334_002581"
     CDS             <1..479
                     /locus_tag="IV334_002581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HAP8944602.1"
                     /translation="TYAMTGWRIGYAVGNPVIINGMIAVASQSTSNPTAVSQYAAVEA
                     LKGEQDTVEEMRNAFEERLNTLYPLVAELPGVSLEKPQGAFYLFPNVKETLRMCKYEN
                     VTQWVEDLLEETGVALVTGEGFGAPENVRMSYATDRATLEEAVRRIKQFIESKSQN"
     gene            519..1817
                     /gene="asnS"
                     /locus_tag="IV334_002582"
     CDS             519..1817
                     /gene="asnS"
                     /locus_tag="IV334_002582"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016180901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HAP8944603.1"
                     /translation="MEQIQIIDSKNHVGETVTIGAWVANKRSSGKIAFLQLRDGTAFF
                     QGVVVKSEVSEEVFQTAKNLHQETSVLVTGEIREDSRSKFGYEIGITNVEVVSESHDY
                     PITPKEHGTDFLMDHRHLWLRSSRQHAIMQIRNEIIRATYEFFNQHHFVKIDPPILTG
                     SAPEGTTDLFETNYFDQKAYLSQSGQLYMEAAAMAFGKVFSFGPTFRAEKSKTRRHLI
                     EFWMIEPEMAFTHQEESLEVQEQYVAFLVQNVLDHCEYALDVLGRDKEVLKTYTKLPF
                     PRITYDDAIELLKKNGFDDIEWGEDFGSPHETFIANSFEQPVFILNYPKAIKPFYMKP
                     HPTREDVVICADMIAPEGYGEIIGGSERATDYDFLLEQIRNHGLDEKEYSWYLDLRKY
                     GSVPHSGFGLGLERTVTWIAGIDHVREAIPFPRLLNRIYP"
     gene            2993..3667
                     /locus_tag="IV334_002583"
     CDS             2993..3667
                     /locus_tag="IV334_002583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HAP8944604.1"
                     /translation="MKKRILFIGDSITDAGRDRAEATDLGKGYPLLIAGKLISQYPTM
                     KFECMNRGIGGDKIADLQVRWQEDCLALQPDYVSILIGINDTWHNTSDQAIFGTEKSA
                     QQFESVYRKLLNELQTAEICNIILMEPFVLPITDDRHGWRKDLDPKIQIVRKLAKEYG
                     ALLVPLDGIFNALGIAYGFSKYTREDGVHPTIGGHEIIAQSWIDAIAAVDFFSGDKNE
                     RKKSEK"
     gene            3664..4005
                     /locus_tag="IV334_002584"
     CDS             3664..4005
                     /locus_tag="IV334_002584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8944605.1"
                     /translation="MMEIRWAVKEDLPQINQLITDHPESFVKAKTAADLNDKNKLLLV
                     AVTKEKRIIGFAQADVQQSEERVDQRPSGKLIQHTLVTKENTEEIQKKFAEYLMLEAK
                     KRRIKSIFAQF"
     gene            4035..4394
                     /locus_tag="IV334_002585"
     CDS             4035..4394
                     /locus_tag="IV334_002585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccF domain-containing protein"
                     /protein_id="HAP8944606.1"
                     /translation="MSCLGNVIWFIFGGLIGGLSWLAAGILWCITIIGIPIGMQCFKI
                     ASLSFAPFGKEVIYHQSGMSLIVNIIWLIVSGLPIALAHVVSGILLCITIIGIPFAKQ
                     SFKLARLALMPFGAEIV"
     gene            4598..4777
                     /locus_tag="IV334_002586"
                     /pseudo
     CDS             4598..4777
                     /locus_tag="IV334_002586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308695.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
BASE COUNT         1608 a          835 c         1037 g         1320 t
ORIGIN      
        1 aaacatatgc gatgacaggc tggcgcattg gttatgcggt gggaaatcct gtgatcatca
       61 atggaatgat cgctgttgct tcccaatcaa ctagcaatcc gacagcagtc agtcaatatg
      121 cagctgttga agcattgaaa ggtgagcaag atacagtaga agaaatgcgg aacgcctttg
      181 aagaacgcct gaatacgtta tatccgcttg tggcagaact gccgggtgtt tctcttgaaa
      241 agccgcaagg agctttctat ctttttccta atgtaaaaga aacgctacgc atgtgtaagt
      301 atgaaaatgt cacacaatgg gtagaagatc ttttagaaga aacaggggta gcgcttgtga
      361 ctggcgaagg ctttggggca ccagaaaatg tccgcatgag ttatgccact gatcgcgcta
      421 cgttagaaga agcggtacgc agaatcaagc aatttattga gagtaaaagt caaaattaat
      481 cctgaagaaa ttcaaggaat ataaaaggag agtcatacgt ggaacaaatt caaatcatcg
      541 attcgaaaaa ccatgtggga gaaacagtaa caatcggcgc ttgggtcgca aataagcgat
      601 caagcggaaa aatcgcattt ttacaattaa gagacggaac agcctttttc caaggtgtag
      661 ttgtaaaaag cgaagtgtcg gaagaagttt tccaaacagc taaaaatcta caccaagaaa
      721 catccgtgtt agtaactgga gaaatccgtg aagacagtcg ttcaaaattt ggttatgaaa
      781 tcggaattac aaatgtggaa gtggtaagcg agagccacga ttatccgatc acaccaaaag
      841 aacacggaac agacttcttg atggatcatc gtcatttatg gctgcgttct tcaagacaac
      901 atgcgattat gcagatcaga aatgaaatca ttcgtgcaac gtatgaattc tttaatcaac
      961 atcattttgt caaaattgat ccgccgatct tgactggttc tgcaccagaa ggcactacgg
     1021 atctattcga aacaaattac tttgatcaaa aagcttattt atctcaaagt ggccaattat
     1081 acatggaagc tgctgctatg gctttcggaa aagtcttttc attcggtccg actttccgtg
     1141 ctgaaaaatc aaaaacacga cgtcatttga tcgaattctg gatgatcgaa ccagaaatgg
     1201 ctttcactca tcaagaagaa agtttagaag tacaggaaca atatgtagca ttcttagtac
     1261 aaaatgtttt agatcattgc gaatatgcat tagatgtact aggacgagac aaagaagtct
     1321 taaaaactta tacaaaattg ccattccctc gtattactta cgatgatgca atcgaattac
     1381 tgaagaaaaa tggatttgat gacatcgaat ggggcgaaga ctttggttca cctcatgaaa
     1441 cgttcattgc gaattcattc gaacagcctg tctttatcct aaactatcca aaagccatca
     1501 agccattcta catgaaacca catccaacaa gagaagacgt ggtaatttgt gcagatatga
     1561 tcgcaccaga aggttacggt gaaattattg gcggaagcga acgggcaaca gactatgatt
     1621 tcttgctaga acaaatccgt aatcacggtt tggatgaaaa agaatattca tggtatcttg
     1681 atctacgtaa atacggttct gttccgcact ctggttttgg tctaggcctt gaacgaacag
     1741 tcacttggat tgctggtatc gaccatgtac gtgaagctat tccattccca cgtttgttga
     1801 accgaattta tccataagag gtgatgaaaa agctgttctg catcaagtaa ttagaacagc
     1861 caagcaaaaa cagctgttca tgtttttgaa ttggcgggac ttattaccgc agatgtaagc
     1921 ttttgaatac cgtttagtgg agaggttgtg aaaaagctgt ttagtttcga gtattaagaa
     1981 aaattttgtg gaaatggctt tccacatttt tgcataaatt tggcttaata ccgagaaact
     2041 ggcttttgaa caccgtttaa ggaagaggtt gtgaaatcag tgccttgacc tgagtattag
     2101 agaaacaaaa cggaaaaata gcttctcata ttttaccgtt tttttagcta atatcgaggg
     2161 tcagctgatt gaacaccgtt tatatcaaga ggttgtgcgc ctgccgttca gctccgagtc
     2221 acaaggagta atttcaaaaa acagtttttc atgtttgatg actattgtga cttgtgaccg
     2281 aggagttggc cggagagcaa cgtttattta aaaaaaagaa ctgcgaactc tcgcagttct
     2341 ttttttaaaa tacgggataa aggaagtttc cagcagacaa gagaagaaag caaaagaaat
     2401 tgctatttga ttcacaaggt tctataacga aatcgcaatc aatcaaacat atacaaagaa
     2461 ggttattcaa atggaaatct gatttgatga tgcttctgct gttgaaataa agcagcatct
     2521 agtacctgaa aacaaactaa tagtaacatt cgaagatgga gtaggtccgt attttcagca
     2581 tgcaatgatc catatgtaag tttaattcat ccttgaaatc atcggaccag ataaagttat
     2641 gaccaatcta tcccaagcaa tctagaagag atattagtaa aagaacattt actggaagtc
     2701 ttagatgagc ctataaatat atgttctgat ggcgtcatca gtggtctgat tgacggaagc
     2761 ttaggtgtga tcgatttctc tggaccaaac aacgaaggaa tcaaggggaa tccggcacgt
     2821 taagaaaaaa agtataaaaa actagacact tgatgagcgt tcattcaatt gattgaaggg
     2881 actaggctgg tgtcattttt tatacatata ttttctcttt cgtcttgata gggcttatgg
     2941 gataatataa ggaagaaaat catcgtttta ttcaaaaagg agtttttgac aaatgaaaaa
     3001 acgtatatta tttatcggtg acagcattac agacgcagga agagaccgag cagaagcaac
     3061 tgatctaggt aaaggttatc cacttttgat tgccggaaaa ctaatctccc aatatccaac
     3121 gatgaaattt gaatgcatga accgtgggat aggaggagac aaaattgcag atctgcaagt
     3181 acgctggcaa gaagattgtt tagctttaca acctgactat gtatcgattt tgatcggaat
     3241 caatgatacc tggcataata ctagcgatca ggctatattc ggaactgaga aatctgcgca
     3301 gcaatttgaa tccgtttatc gaaaattact aaatgaactt caaacagcgg aaatctgcaa
     3361 catcatcttg atggagccgt ttgtacttcc tataaccgac gaccgtcatg gctggcgtaa
     3421 agacttagac cctaaaatcc aaattgttcg taaacttgct aaggaatatg gcgcattgct
     3481 ggttccttta gatggcatat tcaatgcttt agggattgct tatggttttt cgaaatatac
     3541 aagagaagat ggggtccacc caacgattgg cggacatgag atcattgcac aaagctggat
     3601 agatgcaatt gccgcagtcg atttttttag tggagataaa aatgagcgaa agaaaagtga
     3661 gaaatgatgg aaatacggtg ggcagtaaaa gaagatttac ctcaaatcaa tcagctgatt
     3721 actgatcatc cagaatcatt cgtaaaagca aaaacggcag ctgacctaaa tgataagaat
     3781 aaactattgc tcgttgctgt aacaaaagaa aaacggatta tcggatttgc gcaagcggac
     3841 gtgcaacagt cagaagaacg ggtggatcag cgaccatcag gaaaactaat ccaacacaca
     3901 ttagtaacaa aagaaaatac ggaagaaatc caaaaaaaat ttgcagaata cttgatgctg
     3961 gaagctaaaa aaagaagaat aaaatcaatt tttgcccagt tttaatcaaa aacagtaata
     4021 gaaggagaaa tacaatgagt tgtttaggaa atgtcatctg gtttatattc ggcggactaa
     4081 tcggtggtct tagctggtta gcagcgggga tactttggtg tatcacgatc ataggtatcc
     4141 caatcggtat gcaatgtttt aagatcgcct ccctttcttt tgcgcctttt ggcaaagaag
     4201 taatttatca ccagtctggc atgtcattga tcgttaatat catctggctg atcgtttcag
     4261 gcctaccaat tgctctcgca catgtcgtca gcggcatcct tctatgtatc acgatcatag
     4321 ggataccttt tgccaagcaa tctttcaaac ttgctcgatt ggcgttgatg ccattcggtg
     4381 cagaaattgt ataaaaagag aataataaag gttagaaaac gcatagagtc aggtgggata
     4441 catgtatttc acctgactct atgtgttttc ctgtataagt tctcccatgt gacttttacg
     4501 tcttttattt atatgggatt actgtttatg gaaagttgaa ataattttca ttaaagaact
     4561 ctttctatat ttttgaaagc gtagtggagc ttcgaatatc tgggaaggac attttgcagg
     4621 gattgaaaaa agatattgtc ctatcgaaaa attaatgaat ttagacaaat tgccagcaac
     4681 aggcttcaaa cttagttgct atcctgtcaa gatcaagaag gcaagtgcgg gttggataag
     4741 agcaggggca atgatcgaag agaaaaagaa agaataagcc aaaacatctg tatggaagaa
//
LOCUS       DACDKM010000144         4798 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20834_2016
            SAMN12640370-rid9891663.denovo.144, whole genome shotgun sequence.
ACCESSION   DACDKM010000144 DACDKM010000000
VERSION     DACDKM010000144.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640370
            Sequence Read Archive: SRR10040891
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4798)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4798)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742835.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742835.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:04:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,012
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 29 of 75
            Pseudo Genes (incomplete)         :: 33 of 75
            Pseudo Genes (internal stop)      :: 19 of 75
            Pseudo Genes (multiple problems)  :: 5 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4798
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640370-rid9891663.denovo.144"
                     /isolate="E20834_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..960
                     /locus_tag="IV334_002587"
     CDS             <1..960
                     /locus_tag="IV334_002587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019117172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HAP8944607.1"
                     /translation="LKLNSVLGDTKLLPQRKKIENPHPLLQTTVEPSKPEQREMLLDA
                     LLEISDSDPLLRYYVDSTTHEIILSFLGKVQMEVISALLQEKYHVEIELKEPTVIYME
                     RPLKNAEYTIHIEVPPNPFWASIGLSVSPLPLGSGMQYESSVSLGYLNQSFQNAVMEG
                     IRYGCEQGLYGWNVTDCKICFKYGLYYSPVSTPADFRMLTPIVLEQAFRKAGTELLEP
                     YLSFKVYAPQEYLSRAYNDAPKYCANIVNTQLKNNEVIIIGEIPARCIQDYRNDLTFF
                     TNGLSVCLAELKGYQVTTGEPVCQTRRLNSRIDKVRYMFNKIT"
     gene            1058..1246
                     /locus_tag="IV334_002588"
     CDS             1058..1246
                     /locus_tag="IV334_002588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011988843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAP8944608.1"
                     /translation="MINYKWILCPICGNKTRLRIRVDTILKNFPLYCPKCKNETLINV
                     QKMNIITIKEPDAKTQSR"
     gene            complement(1304..1657)
                     /locus_tag="IV334_002589"
     CDS             complement(1304..1657)
                     /locus_tag="IV334_002589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001227349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP8944609.1"
                     /translation="MRKKEDKYDFRAVGLAIKEARMKRGLTREQVGTMIEIDPRYLTN
                     IENKGQHPSTQVLYDLVSLLHVSIDEFFLPTDNLIKSTRRLQVEKYMDSFTDKELSLM
                     EALAKGINEARNIEG"
     gene            2188..2610
                     /locus_tag="IV334_002590"
     CDS             2188..2610
                     /locus_tag="IV334_002590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000804881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HAP8944610.1"
                     /translation="MKPSSFQETIENQFDYICKQVIEDERKDYFKHLDRLSAKEVSFS
                     DIDNYLVNSFSTVDTYVTDFQLFKLFGLRINVESDLLSEALRHLSEKKRNIILLHYFM
                     DMSDVEIAELLNLNRSTVYRHRISGLAMIKEFMKECKE"
     gene            2607..2837
                     /locus_tag="IV334_002591"
     CDS             2607..2837
                     /locus_tag="IV334_002591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002411750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP8944611.1"
                     /translation="MKTAYPRVPFPLIVKATDGDVEAINQIVKHYRGYTSKRSLRRMT
                     DEYGNSHMVIDETLRGRMETKLISKILSFEIK"
     gene            3335..3535
                     /locus_tag="IV334_002592"
     CDS             3335..3535
                     /locus_tag="IV334_002592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008475635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 domain-containing protein"
                     /protein_id="HAP8944612.1"
                     /translation="MITVTKKDLVELGYGTSFSADIIKKAKELMITKGHIYYQSRKLD
                     RVPREAVEEILGIRFSNENRSE"
     gene            3562..4755
                     /locus_tag="IV334_002593"
     CDS             3562..4755
                     /locus_tag="IV334_002593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002388733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAP8944613.1"
                     /translation="MVKDPIKKAKNGTYYFRANLGYDSKGKKIQKYRSGFTTKKEARE
                     EYSKLLLMKPEELSENKDKMTFKHYIFEIFLPWYKTQVKLRTYENRLPTIKKHFSYFD
                     KMAVSDIEPIDVQNWQLKLSKKCKSSYVRAVQGLFSVAMDRAIVLGITTTNPSKIIGN
                     VKKQKSKIEFWTKEEFEKVISLIYKEDYYQHFLFISLWFLFMTGMRIGEATAIQWEDI
                     DFDSGVLTINKTLYYKNQSNYRFVEPKTKASVRHIVLDKCTLTYLSEWKEVQQNLIQT
                     DFVMSYNGIPTQKHTLSYAIERYAKLAGIHRIRIHGLRHSHASLLISMGENPLIIKDR
                     LGHEDIETTLGTYGHLYPNSNFEVANKLNGAISFKEAKQNYDTSPKNQFTVDYLRNKE
                     MKKVQ"
BASE COUNT         1649 a          787 c          883 g         1479 t
ORIGIN      
        1 ttgaagttaa atagtgttct tggagataca aaactattgc cacagagaaa aaagattgaa
       61 aatccgcacc ctctactaca aacaactgtt gaaccgagta aacctgaaca gagagaaatg
      121 ttgcttgatg cccttttgga aatctcagat agtgatccgc ttctacgata ttacgtggat
      181 tctacgacac atgaaattat actttctttc ttagggaaag tacaaatgga agtgattagt
      241 gcactgttgc aagaaaagta tcatgtggag atagaactaa aagagcctac agtcatttat
      301 atggagagac cgttaaaaaa tgcagaatat accattcaca tcgaagtgcc gccaaatcct
      361 ttctgggctt ccattggttt atctgtatca ccgcttccgt tgggaagtgg aatgcagtat
      421 gagagctcgg tttctcttgg atacttaaat caatcatttc aaaatgcagt tatggaaggg
      481 atacgctatg gttgcgaaca aggattatat ggttggaatg tgacggattg taaaatctgt
      541 tttaagtacg gtttatacta tagccctgtt agtactccag cagattttcg gatgcttact
      601 cctattgtac tggagcaagc ctttagaaaa gctggaacag aattgttaga gccatatctt
      661 agttttaaag tttatgcacc acaggaatat ctttcacggg catataacga tgctcccaaa
      721 tattgtgcaa atatcgtaaa tactcaactg aaaaataatg aggtcattat tattggagaa
      781 attcctgctc gatgtattca agattatcgc aatgatttaa ctttttttac aaatgggctt
      841 agtgtttgtt tagcagagct aaaaggatat caggttacca ctggcgaacc tgtttgccag
      901 acccgtcgtc taaatagtcg gatagataaa gtaagatata tgttcaataa aataacttag
      961 tgcgttttat gttgttatat aaatatggtt tcttattaaa taagatgaaa tattctttaa
     1021 tatagatttg aattaaagtg gaaaggagga gattgttatt ataaactaca agtggatatt
     1081 gtgtcctatt tgtggaaata aaacaagact acgaatacga gtggatacta tacttaaaaa
     1141 tttcccttta tactgcccca aatgtaagaa cgaaacttta attaatgttc aaaaaatgaa
     1201 tataataaca atcaaagagc cagacgccaa gacgcagagc cgataatttg agaaatgaaa
     1261 ctctcatctt atcggctctt tttgtttatc tgaattttac tgactagcct tcaatatttc
     1321 ttgcttcatt gatacctttt gctagtgctt ccattaagga tagttctttg tctgtaaagc
     1381 tatccatgta tttttctacc tgtaaccttc gagtgctttt aatcaaatta tctgtaggta
     1441 agaaaaattc atcaatagat acatggagta atgatacaag atcatacagc acttgtgtac
     1501 ttgggtgttg tcctttgttt tctatatttg ttaaataacg tgggtcaatt tcaatcattg
     1561 ttcccacttg ttcacgagtg agaccccgtt tcattcgagc ctctttaatc gctaaaccaa
     1621 cggctctaaa atcgtattta tcttcttttt tacgcatact aaatcacctc tatacatttt
     1681 actgttccta tataaccata aacaggtata gaaaaacatg ttatatagtt tatgagttcc
     1741 tatagaacac tgtggaattt attaatgcga tgaatccaat ttcaaataga aaggtcgctg
     1801 attttattta cctatacaac tttataaaat ttagtcatac tcctagaaag tagataaaat
     1861 tggcgactta atggagggaa aagtcgccat gagaaagata atctgtcatc tagttctctt
     1921 aattaagttc ttatcaaaaa aacttatcca cctgtactgg atagatatga ccatcaatgt
     1981 ggttttgcag attacataga tagaaaaatc aaagcatatg aacagagaaa acaatctgtt
     2041 tatgtgcttt tttggtcact taataaatat cttttaggat ttctcttcat ttatgcaaca
     2101 aatcccccgt tcgtgtcggg gtttagtatg aagggagata aagaagcaca tgccactccc
     2161 tttcacgaca gaaagggggt gaaaagcatg aaaccatctt cttttcaaga aaccatagaa
     2221 aatcagtttg actatatctg caaacaagtt attgaagatg aacgaaaaga ttacttcaaa
     2281 catctagaca gactatcagc aaaagaagtc tcgttttctg atatagataa ctaccttgtc
     2341 aactcttttt ctactgttga tacgtacgtc actgatttcc aactatttaa attatttgga
     2401 ttaagaatca atgttgaaag tgatttatta agtgaagccc tgcgtcatct ttcagagaaa
     2461 aaacggaaca ttattttact tcattacttt atggatatga gtgatgtgga gattgcggag
     2521 ttactaaatt tgaatcgttc gactgtttat cgtcatcgaa taagtggact agccatgatt
     2581 aaagaattca tgaaggagtg taaagaatga aaacagctta tccaagggtt cccttccccc
     2641 tcattgtaaa ggcgacagat ggcgacgtgg aagcaattaa tcagattgtt aaacattatc
     2701 gagggtatac ctcaaaacgt tcattacgtc gcatgacaga cgaatatgga aattctcata
     2761 tggttattga tgaaacccta cgtggcagaa tggaaacgaa actcatctct aagattttat
     2821 cgttcgaaat taaatagtcc cattttttcg tggaagcgtg tcgaatcgta tccacgcttc
     2881 ccgatttggg aggtttgtta ttccatcaaa gcatattgtg tgttcagtag gttttgatag
     2941 gctaacaagc caaacgttct ttgacaacca aataaaggca atcgaatacg tttcggtaag
     3001 aaaagagctg atgaactaac acgccatgac ctacaaaatt agtgagcgat tcatgttagt
     3061 aattcgaaag taatctttag caggattgct gccatgactt tcttatcgtg ataatgatac
     3121 tcccgtatag ccatagtccg agcgttagaa gcgtcacagg caatgggtac ggctacatga
     3181 gaaccatgca gaagtgaaat tcctgtgagc tttgctagag ctgttcgatt gctaagatat
     3241 tcttctcttg tcagtgtatt attataaaac tatttgatat aattataggt gaatattgga
     3301 gttttcctag cctataattg ggaaaggagt aataatgata acggtcacaa aaaaggattt
     3361 agttgagcta ggatatggaa cttctttttc agcagacatc ataaaaaaag caaaagaatt
     3421 gatgattaca aaagggcata tctactatca atctcgaaag ctagatagag tccctcgtga
     3481 agctgttgaa gaaattctag gaattcggtt ttcaaatgaa aacaggtctg agtagttttt
     3541 gcacagtaag gagtaaaaac tatggttaaa gacccaataa aaaaagcaaa gaatggaacc
     3601 tattattttc gtgcaaatct aggttatgat tctaaaggga aaaaaattca aaaatatcgt
     3661 agtggattca ctactaaaaa agaagcaaga gaagaatatt cgaaactgct attgatgaaa
     3721 ccagaagaat taagtgagaa taaggataaa atgacgttta aacattatat ctttgaaatt
     3781 ttccttccat ggtataagac acaggtaaag ctcagaacat atgaaaatcg cttaccaact
     3841 attaagaagc atttctctta ttttgataaa atggctgttt ccgatattga gcctattgat
     3901 gtgcaaaatt ggcaactaaa attatcaaag aagtgtaagt cttcttatgt aagagctgtt
     3961 caaggactat tttcagttgc tatggatcga gcaattgtcc ttgggataac caccacaaat
     4021 ccatctaaga ttattggcaa tgtgaaaaag caaaagtcta aaattgaatt ttggacaaaa
     4081 gaagaatttg aaaaagtaat ctctctaatt tacaaagaag attactatca acacttttta
     4141 tttatctctc tgtggttttt atttatgact ggaatgcgaa ttggtgaagc tactgccatt
     4201 caatgggaag atattgattt tgactcagga gtactaacca tcaacaaaac actctattac
     4261 aagaatcaat cgaattatag atttgttgaa ccaaaaacaa aggcgagcgt tcgtcatatt
     4321 gtattagata aatgcacatt aacttattta tccgaatgga aggaagtaca acaaaatcta
     4381 attcagacag actttgtaat gagctataac ggtattccta cccaaaaaca tacactatca
     4441 tatgctattg aacgctatgc taagctggct ggtatccatc gaatcagaat ccatgggtta
     4501 cggcattctc atgcttcctt gttaatcagt atgggagaaa atccacttat cattaaagat
     4561 cgtctaggac acgaagatat tgagacgact ttaggaacgt atggacatct ttatcctaac
     4621 agtaatttcg aggtagctaa caaattaaat ggagccattt cctttaaaga agcaaaacaa
     4681 aattatgata cttcaccaaa gaatcaattt actgtagact acttgcgaaa caaagaaatg
     4741 aaaaaagtgc aataaaagtg cagtaaaaag ataagaagag ccagaaaccc ttggtata
//
LOCUS       DACDKM010000145         4788 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20834_2016
            SAMN12640370-rid9891663.denovo.145, whole genome shotgun sequence.
ACCESSION   DACDKM010000145 DACDKM010000000
VERSION     DACDKM010000145.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640370
            Sequence Read Archive: SRR10040891
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4788)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4788)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742835.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742835.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:04:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,012
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 29 of 75
            Pseudo Genes (incomplete)         :: 33 of 75
            Pseudo Genes (internal stop)      :: 19 of 75
            Pseudo Genes (multiple problems)  :: 5 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4788
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640370-rid9891663.denovo.145"
                     /isolate="E20834_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            564..989
                     /locus_tag="IV334_002594"
     CDS             564..989
                     /locus_tag="IV334_002594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8944614.1"
                     /translation="MICKNKKKIIAIIASFSLIFSTACTVLLTNNANVEAASSSYKHN
                     HAGYRCYKMSYYVSPSKCKQLARQTNKLNTVSAIANFIGLSGLTPGIISLVFGSAVSA
                     NNVFVTAARRGKGVQLNYIAHFSNTTTYNYSSNQNYVIK"
     gene            2141..3118
                     /locus_tag="IV334_002595"
     CDS             2141..3118
                     /locus_tag="IV334_002595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016186668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HAP8944615.1"
                     /translation="MANEIVKYHHELNTIPLRKFTSVEMNLFFSIVSRMRDVGDKKVQ
                     FTFEQLKDLSNYKATANVRFIDDLETTYDKLMDLRFGRRSADGLQRERFVLFNQFKID
                     GKADIPFAEIQVHEKALPLLNNLEEWVRYSLQQFNELESSYSKTMFRLLKQYRTKGIA
                     YFSKEDFHELLDIPKSYRESDINKKIIKPIRQELTAIFKGLTIKKKYGKGRGKPVIGY
                     QFTFKPEIKKADDFNKHAPTKEEQNKALELVNQQIYSEKDPTSLQEKIKEQKRKLELL
                     QQLEELERKQNNLNKENKEINEQIPDNNSDIPPELKKGLLDNLQNLFKE"
     gene            3128..3625
                     /locus_tag="IV334_002596"
     CDS             3128..3625
                     /locus_tag="IV334_002596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP8944616.1"
                     /translation="MTDKTIKELADILGVSKDKVKYQLRKLPSNFTYKKGNIIYLKKE
                     AVTTITKVLSGNEPGNLPSELPDSLPTDYLLKEIETKNNHIEEKDRQIEKLQLALETQ
                     QKLLDQQQQLTLQANQQIEKLQEQLQLTYTEESSENKSTTLSEETESIEKQSKTEKKW
                     WQFWQ"
     gene            4001..4282
                     /locus_tag="IV334_002597"
     CDS             4001..4282
                     /locus_tag="IV334_002597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8944617.1"
                     /translation="MDILVRNINPKIITRIDKRCQELTTKTGKKWSRNQYLKILIEND
                     FDHELMNYKKDQFDLLFEKFIAIQAENTNILNDYVHTNNQLIETLIGGI"
     gene            4284..4700
                     /locus_tag="IV334_002598"
     CDS             4284..4700
                     /locus_tag="IV334_002598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001232859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8944618.1"
                     /translation="MRKTSVQKHVTLYPSHVDVLDSIIEKNPDVNNYSEAIRFLCMSY
                     EPATNTTTTSNQAKLNAMGKEISIMTEIMAEFADLYLKDTGVLIGTNSKVYQEVKEKI
                     ENQLNPSRRNQPKNRQKETNQTFTSGNFDPTNFLKR"
BASE COUNT         1757 a          816 c          786 g         1429 t
ORIGIN      
        1 aaaaaaaaaa gaaaaatccg cttctgcgaa ttcttctttt ttcattttgg ggcgctgccc
       61 taatccctaa gtcccggtta ttcaatagca tggaaactca aagaataaga cgttccctgg
      121 aataaaaaaa aggctgctcg ttcgagcagt ccatacatag gaaaatgaaa aaacgacgtc
      181 ccttactgag aaacgctatt aaacttttaa caaatcctct aaaaaataac aagtctttaa
      241 aaagtgaccg gtaaaaatgc acaaagaact aaaaaagaga aattgcatca aatcaagcat
      301 tagaagctag taactagcta gaccaattga aaatcacaaa gtgagcgcct gtatctattg
      361 ctacaggcgc tacttggcat agtgtacatt atgtcaacaa gaatttaata taacatattt
      421 ttctagtttg ttataacaat gccactctaa caaaaatgta agcattatcc ttattaaaag
      481 ctccttgaca aggactatac cttttatttt atgattttta catagaaaac gttttctcta
      541 ttttttgttt agaggtgatt tttatgattt gcaagaacaa gaaaaaaatt attgctatta
      601 ttgctagttt ctctttgatt tttagtactg catgtactgt cttattaaca aataatgcta
      661 atgttgaagc tgcaagctct tcttacaaac ataatcatgc tggttataga tgttacaaaa
      721 tgagttatta tgtaagtcct tctaaatgca aacaattggc tcgccaaact aataaactta
      781 ataccgtctc agctattgca aactttattg gactatctgg attaacaccg gggattatat
      841 cattagtttt cggatcagca gttagtgcta ataatgtatt tgttacagcc gctagaagag
      901 gaaaaggcgt tcaattaaat tatattgccc attttagtaa taccactacc tataattata
      961 gttctaacca aaattatgtt ataaaataat taaaacttta aactaggagg aatgctttat
     1021 gaaattaaca aaaattgctg taattttagc tgttattgct ggcattattt tatattttgt
     1081 attgcctgct aatcaattct tcctttactt tggaatacct gcgattttaa tagtaaatgg
     1141 cgtaagaatc tttgaacaac gaaaaaaatg tagctctacc taatcgttac aaaacaacaa
     1201 tttcaccttc ggtgaaataa gcgaaacttt acaaaaagtg gctagcaaat gctagccttt
     1261 tttgtattgt agagcgtgaa ttttgagggg gattcaaggg ggagcttgcg cccctttgac
     1321 aagcggttat ttgcaccaaa tacctccgta tttggctagc aatccttcta aaaaatacac
     1381 ctatcgatca gagtcaaaac aaatcaattt tcgagtgagc gaatccgaat ttgcaaagct
     1441 gaagctttgc aaatgagtgt gccggcttcc gtaaaaatac atatggctat aatttgcgtt
     1501 ttaaggcgtt ttaaacgttc tggtatataa ttataccaaa acaactttaa aacgccttag
     1561 atctaatttt agaggtctta tagaacattt tagggttgtt agggcttgcc ctgaccgcct
     1621 gtaaggcgct ttgagaacaa gataagggtt ttatactaag actgtcttaa aacggcttaa
     1681 acaaacgctc agaacttttc aaaggagttc tttatgaact gtttttaagc aaaggagcct
     1741 ttaggcgact gcgcagtata atgtgagagc gtagcgatca cacattttta gtgaccgtag
     1801 ggagcgagtt tcccagttta gaaaaattcg cgaacttgtt tgaggaaaaa agcaggagtt
     1861 ttttaggcgg gaaattttgc ctcctctttt ataatctctt tttaaacctc tttttaaacc
     1921 tctttaccgt cggctgtatg cttactctcc caaggtgtgc agaatctttt gggggtcttt
     1981 ttgtctgttt ttaggggtct ttttgtctgt ttttaggggt ctttttgtct gtttttgggg
     2041 gtctttttgt ctgtttttag gggtctttaa ttaggggctt gacccctatt agaaagctct
     2101 ttataatttt gttaagtagt taatcttgaa ggaggatttt atggcaaatg aaattgtgaa
     2161 ataccaccat gaattaaata cgattccttt acgcaaattt acgtcagtag aaatgaattt
     2221 attcttctct atagtatcta gaatgcgtga cgtaggcgat aaaaaagtac aatttacatt
     2281 cgaacaatta aaagatttaa gcaattataa agcaactgca aatgttcgat tcatagatga
     2341 tttagaaaca acgtatgaca aattaatgga tttacgtttt ggtagaagaa gtgctgacgg
     2401 tttgcaacgt gaacgatttg ttttatttaa tcaatttaaa atcgatggaa aagctgatat
     2461 tccttttgct gaaatacaag tccatgagaa agccttaccc ttacttaata atcttgaaga
     2521 atgggttcga tattctctcc aacaattcaa tgaattagaa agcagttatt ccaaaaccat
     2581 gtttagatta ttaaaacaat acagaaccaa aggaattgcc tatttttcaa aagaagactt
     2641 tcatgaatta cttgatatcc ctaaaagtta tagagaaagt gatataaata aaaaaattat
     2701 aaagcctatt cgccaagaac taaccgcaat cttcaaaggt ctgacaatca aaaagaaata
     2761 cggaaaagga agaggaaaac ctgtcattgg ctatcaattc acgtttaaac ctgaaataaa
     2821 aaaagcagac gactttaata aacacgcacc aacaaaagaa gaacaaaata aagctttaga
     2881 actagttaat caacaaattt actctgaaaa agaccccact tcattacaag aaaaaatcaa
     2941 agaacagaaa cgaaaattag aacttttaca acaattagaa gagctagaaa gaaaacaaaa
     3001 taatctgaat aaagaaaata aggagattaa cgaacaaata ccagataaca attccgatat
     3061 tcccccagaa ttaaaaaaag gcttactaga taatcttcaa aacttattta aagaataagg
     3121 ggtttttatg acagataaaa ctatcaaaga attagcagat attctaggtg tttcaaaaga
     3181 taaagtgaag tatcaactga ggaaattacc tagtaatttt acctacaaaa aaggaaatat
     3241 tatctacctg aaaaaagaag cagtaacaac aattacaaag gttttatcag gtaatgagcc
     3301 gggtaactta cctagtgaat tacctgattc attacctacc gattacctac tcaaggagat
     3361 tgaaactaag aataatcata ttgaagaaaa agataggcaa attgaaaaac ttcaactagc
     3421 tttagaaaca caacaaaaat tactcgacca gcaacaacaa ctcaccctgc aagccaacca
     3481 acaaatcgag aaactgcagg aacaactaca gttgacttat acggaagaat cttcggaaaa
     3541 taaatcaacg actctttctg aagaaacaga atcgatagaa aaacaatcaa agacggagaa
     3601 aaaatggtgg caattctggc aataaaacca gcaatttaaa ttaataaaaa ttgctggttt
     3661 tattcttctt tttgcttttg cgagcagagc aacatgggcc catgttgcga tattttggtg
     3721 gcgccccaat ccccgttttt tcgttgaatc ttactcggac tgcactcgtt ttacagctga
     3781 tttttctgaa ttttgaaaga gcataaggaa acgtggtatc actttcttac gaaaggtgct
     3841 agataagctg tgcctatcac gtttctcaga aaaaaatttg ctatcacttt tttgccattg
     3901 tggctaggct atgtatagtg ttttggcgaa aaatagctat acaaatacta tatttgtatg
     3961 atatacataa attatatact gatacgaaag gaaaatttcc atggacattt tagttcgaaa
     4021 tatcaatccc aaaattatta caagaataga taaacgttgt caggaactaa caaccaaaac
     4081 gggcaaaaaa tggagtcgaa atcaatacct aaaaatttta atcgaaaacg attttgacca
     4141 cgaacttatg aattacaaaa aagatcaatt cgatttatta ttcgaaaaat ttattgccat
     4201 tcaagcagaa aatacgaaca tactaaatga ctatgttcac acaaacaacc aactcattga
     4261 aacacttatc ggaggaatat aacatgagaa aaacttctgt tcaaaaacac gtaacccttt
     4321 atcccagtca cgttgacgtt ttagattcca tcatcgaaaa aaatccagac gtcaacaatt
     4381 attcagaagc cattcgcttt ttatgtatga gttatgaacc tgctaccaat acaaccacaa
     4441 ccagtaacca agcaaaactc aatgccatgg gaaaagaaat ttctatcatg acagaaatca
     4501 tggcagaatt tgctgattta tatctaaaag atactggtgt tcttatcggt acaaattcta
     4561 aagtatatca agaagtgaaa gaaaaaattg aaaatcaact caatccttcc cgacgaaatc
     4621 aacccaaaaa tcgtcaaaaa gaaaccaatc aaacctttac ttctggaaat tttgacccaa
     4681 caaactttct aaaaagataa aaaaagaaac cgaaaaataa aaaaattcct cgatttcttt
     4741 ttagggatcg gtcttccagc aagatttgca agttggtacc aacttgcg
//
LOCUS       DACDKM010000146         4711 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20834_2016
            SAMN12640370-rid9891663.denovo.146, whole genome shotgun sequence.
ACCESSION   DACDKM010000146 DACDKM010000000
VERSION     DACDKM010000146.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640370
            Sequence Read Archive: SRR10040891
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4711)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4711)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742835.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742835.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:04:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,012
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 29 of 75
            Pseudo Genes (incomplete)         :: 33 of 75
            Pseudo Genes (internal stop)      :: 19 of 75
            Pseudo Genes (multiple problems)  :: 5 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4711
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640370-rid9891663.denovo.146"
                     /isolate="E20834_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            39..1118
                     /locus_tag="IV334_002599"
     CDS             39..1118
                     /locus_tag="IV334_002599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HAP8944619.1"
                     /translation="MLYYISEGGRKVKVLLYFESEKMLAKSGIGRALDHQKRALTEVG
                     ISYTLDEKEDYDILHINTYGINSHNMVNKARREGKKVVYHAHSTEEDFRNSFIGSNQL
                     SPIVKKYLVGLYQKADYLITPTPYSKQLLESYGIRVPIQAISNGIDLEKYHPDPMKEQ
                     KFREYFKLSPDQKVIICVGLFFERKGIIDFVEIAKKMPEYTFIWFGHVPMYSIPRNIR
                     KIVKEDHPENVLFPGYIRGEIIEGAYSGADLFFFPSYEETEGIVVLEALASKQNVLVR
                     DIPVYNGWLEDSKNCYMGKTNEEFIRLIQQITNQELPSTTQAGYQTAKERSINKIGEE
                     LKNVYESVLNEKVSSKLKKVNQVKD"
     gene            1135..2358
                     /locus_tag="IV334_002600"
     CDS             1135..2358
                     /locus_tag="IV334_002600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HAP8944620.1"
                     /translation="MKIGFFTDTYFPQVSGVATSIKTLKQELERHGHEVYIFTTTDPN
                     ADKLEKDVIRMPSVPFISFKDRRVVVRGMWYAYLVAKELELDLIHTHTEFGAGILGKM
                     VAKKLKIPLIHTYHTMYEDYLHYIGKGKVIRQSHVKYLSRLFANHTTGVVCPSERVID
                     TLRSYGVIAPLRVIPTGIDIEKFKRSDITQQDISKLRESLGLQENHLMILSLSRISYE
                     KNIQALINGFPQVISAYPNARLVIVGKGPYVEELEELISELQLEEFVQFTGEVDNNDV
                     ALYYKAADYFVSASTSETQGLTYTEAMAAGTPCVVEGNAYLNRLFDHPSLGKTFETDE
                     DFAPALIDYISSEVPKDPKVLEAKLYDISSTHFGEAMIEFYQDMVVYHEEEMRRKEEE
                     DSIERIKIKLNSFKR"
     gene            2447..3334
                     /locus_tag="IV334_002601"
     CDS             2447..3334
                     /locus_tag="IV334_002601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HAP8944621.1"
                     /translation="MKLGFIGTGVMGSAVARHLLEAGHEVAVYNRTKAKADPLVTEGA
                     IWADTPKAVAEQSNILFTMVGYPKDVEEIYYGQSGIFSADISGHILVDLTTSTPSLAE
                     KIAKTAKEKGADALDAPVSGGDLGAKNGTLTIMVGGEEAVYDQVLPLFKEFGTTFTLH
                     GSAGKGQHTKMANQIMIAGTMTGMTEMLVYAQKNGLDLKKVIETLSGGSAANWSLSNY
                     SPRILKEDYTPGFFVKHFIKDLKIALEEAEKMDLVLPATTQALKLYEELADKGFENDG
                     TQALIKLWWPEGKIPEKKS"
     gene            3420..3650
                     /locus_tag="IV334_002602"
     CDS             3420..3650
                     /locus_tag="IV334_002602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkuJ family protein"
                     /protein_id="HAP8944622.1"
                     /translation="MTHSQLVAIIKRLEAMVESADNELQVRRFEKEGVEKCTVTYDKA
                     TDTFELTETDTHQQYEFDNIDIVAMEIYDLIQ"
     gene            complement(3707..4672)
                     /locus_tag="IV334_002603"
     CDS             complement(3707..4672)
                     /locus_tag="IV334_002603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HAP8944623.1"
                     /translation="MLKEKMKTLPMLPGVYLMKDRDGKVIYVGKAKRLKNRVSSYFHQ
                     NKQHSKKVLRMIHHITDFDFVVVDTELDALLLECQLIQHYRPFYNRQMNYFSNYNYVH
                     ITNKGFVLTDTPTARTYGPFRLYKKMPSILRIMEETYQMPWLSEISLLALRVQLPDLQ
                     EMTFEQKKKELQGLFQGRNKKLLTYLKKRQQHFIYQLNFEKAGMLQKDIELVTYFIRR
                     IQEQKQFLRTPSLTFSMPLAADESQKKHYLVCYGQLAETMITSGDVSPDFYYEKKEAH
                     LSLKRQLSKEEIDPVQILISYRKKLEKEQFEIELLNKKEAEKQLN"
BASE COUNT         1591 a          787 c          959 g         1374 t
ORIGIN      
        1 aaaaataggg cttaagtttg agtgacagtt tgtaggctat gttatactat ataagtgaag
       61 ggggacgtaa ggtgaaggta ttattatatt ttgaaagtga aaagatgctt gcaaaatctg
      121 gaatcgggcg tgcgcttgat catcaaaaac gtgctctgac ggaagtcggt atctcttata
      181 cactagatga aaaagaagat tatgacatcc tgcacatcaa tacatacgga atcaatagtc
      241 ataatatggt caataaagcc agaagagaag gaaaaaaagt tgtctatcat gcgcattcta
      301 cagaggaaga tttccgaaat tcctttatcg gatcgaacca gctttctcct atcgtaaaaa
      361 aatatctggt aggattgtac caaaaagcgg actatctgat cacgccaaca ccttattcaa
      421 aacagttgtt ggaaagctat ggtatccgtg tgccgattca agctatttcc aatgggatcg
      481 acttagagaa atatcaccct gatccaatga aagaacaaaa gttcagagaa tatttcaaac
      541 tgtctccgga tcaaaaagta atcatttgtg tcggtttgtt tttcgaacgt aaaggaatca
      601 ttgatttcgt agaaattgct aagaagatgc ccgaatacac atttatttgg tttggtcatg
      661 ttcccatgta ctcgattcct cggaacatcc ggaaaattgt aaaagaagat catcctgaaa
      721 atgtcctgtt tcctggttac attcgtggag agatcataga aggtgcgtat tcaggtgcag
      781 atctattctt tttcccttct tatgaagaga cagaagggat cgttgtttta gaagctttag
      841 caagcaaaca aaacgtactt gttcgagata ttccagtgta taatggctgg ttagaagata
      901 gcaagaactg ctatatgggg aagacaaacg aagaatttat ccgactgatc caacagatta
      961 ccaatcaaga actaccgagt acgacacaag ccggttatca aacagcaaaa gagcgaagta
     1021 tcaataaaat cggtgaagaa cttaaaaatg tatacgaaag tgtactgaat gaaaaagtct
     1081 ctagcaaatt aaaaaaagta aatcaggtca aggactagtt tggaggcgaa aaacatgaaa
     1141 atcggctttt ttaccgatac ctactttccg caagtcagcg gagttgctac atcgatcaaa
     1201 acattaaaac aagaattaga aagacatggg catgaagtct atatttttac aacgaccgat
     1261 cctaatgcgg ataagttgga aaaagatgtg attcgaatgc caagtgtacc atttatttct
     1321 ttcaaggatc gaagagttgt tgttagaggc atgtggtatg cttatttggt tgccaaagaa
     1381 ctagaactag atcttattca tacccataca gaattcggag cagggatact tgggaagatg
     1441 gtagctaaga agttgaaaat acctctgatc catacttacc atacgatgta tgaagactac
     1501 cttcattata tcggtaaagg aaaagtgatc aggcaatccc atgtaaaata cttgtctaga
     1561 ttatttgcaa atcatacgac aggtgtagtt tgtccaagtg aacgtgttat tgatacatta
     1621 cgcagctatg gtgtaatagc accgcttcgc gtgattccta ctggtatcga cattgaaaaa
     1681 ttcaaacgtt ctgatatcac tcagcaggat atcagcaaat taagggagtc actaggtctt
     1741 caggaaaacc atttgatgat tctttcttta agcaggattt cttatgagaa aaatatccaa
     1801 gccttgatta atgggtttcc tcaggtcatc tccgcttatc ctaacgccag attagttatc
     1861 gtaggaaaag gaccttatgt agaagagtta gaggaattga tctcagaact gcagttagaa
     1921 gaatttgtgc agttcacagg agaagtggac aacaatgatg tagcacttta ttataaagcg
     1981 gcagattatt ttgtcagtgc ttctacttca gaaacacaag ggcttactta tacagaagcg
     2041 atggcagccg gaacaccttg tgtcgtggaa ggaaatgctt atttgaaccg attgttcgat
     2101 catccttcat tagggaaaac ttttgaaacc gacgaagact ttgcaccagc acttattgat
     2161 tatatttcca gtgaagttcc taaagatcca aaagttttag aagctaaact ttatgatatt
     2221 tcatctacac atttcggcga agctatgatc gaattttatc aagacatggt tgtttatcat
     2281 gaagaagaga tgcgaagaaa agaagaagaa gactcgattg aacgaatcaa aataaaatta
     2341 aattcattta aaagataaaa ttcaaacatt ttttatgaaa aaatccttgt ttctagcgta
     2401 tactagcata gaagcaagga tttttgattt ttaggaggtc gaaaaaatga aacttggttt
     2461 tatcggaaca ggcgtcatgg gaagtgctgt cgcacgccat ctactagaag caggacacga
     2521 agtagctgtc tataatcgga caaaagcaaa agctgatcct ttagtaacag aaggagctat
     2581 ctgggcagat acaccaaaag cggtagcaga acaaagtaat attctcttta caatggtagg
     2641 ttaccccaaa gatgtagaag aaatctacta tggacagtcg gggatttttt ctgcagatat
     2701 ctcaggacat attttagttg atttgactac aagtacacca agcctggcag aaaaaattgc
     2761 aaaaacagca aaagaaaaag gcgcagatgc gttagatgca ccggtatccg gtggtgactt
     2821 aggagcaaag aatggtacac tgacgatcat ggtcggtgga gaagaagccg tttatgatca
     2881 agtactgcct ttattcaaag aatttggtac gacatttaca ttgcatggtt ctgctgggaa
     2941 aggtcagcat acaaaaatgg ctaatcagat catgattgca ggtacgatga caggaatgac
     3001 ggaaatgctt gtctatgcac aaaaaaacgg acttgattta aagaaagtga ttgagacgct
     3061 ttcaggtgga agtgcagcta actggtcttt aagcaattat tctcctcgta ttttaaaaga
     3121 agattacaca cctggttttt ttgtcaaaca ttttatcaaa gatttaaaga ttgcattaga
     3181 agaagcagaa aaaatggatc ttgtattgcc agcaactacc caagcattga aactatatga
     3241 agagttggca gataaaggtt ttgagaatga cggaacccaa gctttgatca aactttggtg
     3301 gcctgaagga aaaataccag aaaaaaaatc ataaaaaacg agttttttta gagaaaatca
     3361 atgcaagtag gctatgcttt tgatacaata taacagaaat aagaaaagga ggacctccga
     3421 tgacacattc acaattagtc gcaatcataa aacgactgga ggctatggtc gagtcagcag
     3481 ataatgaact gcaagttcgc cgttttgaaa aagaaggcgt agagaagtgt acagtaactt
     3541 atgataaagc gaccgataca tttgaattaa ctgaaacaga tactcatcag caatacgaat
     3601 ttgataatat cgatatcgta gcgatggaaa tctatgactt aatccaataa attttaggat
     3661 aattgacatt tgaagttgaa gatgcctaaa agaagccgag aagcaactaa tttagttgct
     3721 tttcagcttc ttttttgttt aacagttcaa tttcaaactg ttctttttct aattttttgc
     3781 ggtaactgat caagatttgt acaggatcga tttcttcttt tgacaattga cgtttcaaag
     3841 ataaatgagc ttctttcttt tcataataga agtcaggaga aacgtcacca gaggtaatca
     3901 ttgtttctgc aagttgacca taacaaacga ggtagtgctt tttctgactc tcatctgctg
     3961 ctaatggcat gctgaaggtg agcgatggtg ttcttaaaaa ctgcttttgt tcctggatcc
     4021 gtctaataaa ataagtaact aattcgatat ctttttgaag cataccggct ttttcgaaat
     4081 tcaactgata gatgaaatgt tgttgtcttt ttttcaagta agtcagtagc tttttatttc
     4141 ggccttgaaa cagcccttgg agttcctttt ttttctgttc aaaagtcatt tcctgtaggt
     4201 caggaagctg gactcgcaaa gctaacaaac taatttcaga caaccaaggc atttgataag
     4261 tttcttccat tatacgcaaa atagacggca tttttttata gagacgaaaa ggcccgtatg
     4321 ttcgagccgt aggagtatct gttaatacaa atcccttatt ggtaatatgt acataattat
     4381 aattactgaa ataattcatt tgtcggttat aaaaaggacg gtaatgttgg atcaattgac
     4441 actcgagcaa taaagcatct aattccgtgt ctactacgac gaaatcaaaa tctgttatat
     4501 gatgaatcat acgtaatact ttttttgaat gctgtttatt ctgatgaaaa tagctgctta
     4561 ctctattttt gagccgtttt gctttaccaa catagatgac cttaccatct cggtctttca
     4621 taagatagac acccggcagc atggggagag ttttcatttt ttcttttaac ataggatctc
     4681 cttttacgct ttgaataaaa agactataac a
//
LOCUS       DACDKM010000147         4709 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20834_2016
            SAMN12640370-rid9891663.denovo.147, whole genome shotgun sequence.
ACCESSION   DACDKM010000147 DACDKM010000000
VERSION     DACDKM010000147.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640370
            Sequence Read Archive: SRR10040891
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4709)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4709)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742835.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742835.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:04:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,012
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 29 of 75
            Pseudo Genes (incomplete)         :: 33 of 75
            Pseudo Genes (internal stop)      :: 19 of 75
            Pseudo Genes (multiple problems)  :: 5 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4709
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640370-rid9891663.denovo.147"
                     /isolate="E20834_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            105..356
                     /locus_tag="IV334_002604"
                     /pseudo
     CDS             105..356
                     /locus_tag="IV334_002604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627290.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
     gene            449..1243
                     /locus_tag="IV334_002605"
                     /note="aph(3')-IIIa"
     CDS             449..1243
                     /locus_tag="IV334_002605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase
                     APH(3')-IIIa"
                     /protein_id="HAP8944624.1"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLK
                     MTDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEY
                     EDEQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWE
                     EDTPFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKW
                     YDIAFCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     gene            1653..1764
                     /locus_tag="IV334_002606"
                     /pseudo
     CDS             1653..1764
                     /locus_tag="IV334_002606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008787626.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-binding protein"
     gene            1785..1868
                     /locus_tag="IV334_002607"
     CDS             1785..1868
                     /locus_tag="IV334_002607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA methyltransferase attenuator leader
                     peptide ErmL"
                     /protein_id="HAP8944625.1"
                     /translation="MLVFQMRYVDKTSTVLKQTKNSDYADK"
     gene            1993..2730
                     /gene="erm(B)"
                     /locus_tag="IV334_002608"
     CDS             1993..2730
                     /gene="erm(B)"
                     /locus_tag="IV334_002608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004292767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(B)"
                     /protein_id="HAP8944626.1"
                     /translation="MNKNIKYSQNFLTSEKVLNQIIKQLNLKETDTVYEIGTGKGHLT
                     TKLAKISKQVTSIELDSHLFNLSSEKLKLNTRVTLIHQDILQFQFPNKQRYKIVGNIP
                     YHLSTQIIKKVVFESRASDIYLIVEEGFYKRTLDIHRTLGLLLHTQVSIQQLLKLPAE
                     CFHPKPKVNSVLIKLTRHTTDVPDKYWKLYTYFVSKWVNREYRQLFTKNQFHQAMKHA
                     KVNNLSTVTYEQVLSIFNSYLLFNGRK"
     gene            complement(2993..3856)
                     /locus_tag="IV334_002609"
     CDS             complement(2993..3856)
                     /locus_tag="IV334_002609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin zeta"
                     /protein_id="HAP8944627.1"
                     /translation="MANIVNFTDKQFENRLNDNLEELIQGKKAVESPTAFLLGGQPGS
                     GKTSLRSAIFEETQGNVIVIDNDTFKQQHPNFDELVKLYEKDVVKHVTPYSNRMTEAI
                     ISRLSDQGYNLVIEGTGRTTDVPIQTATMLQAKGYETKMYVMAVPKINSYLGTIERYE
                     TMYADDPMTARATPKQAHDIVVKNLPTNLETLHKTGLFSDIRLYNREGVKLYSSLETP
                     SISPKETLEKELNRKVSGKEIQPTLERIEQKMVLNKHQETPEFKAIQQKLESLQPPTP
                     PIPKTPKLPGI"
     gene            complement(3858..4130)
                     /locus_tag="IV334_002610"
     CDS             complement(3858..4130)
                     /locus_tag="IV334_002610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HAP8944628.1"
                     /translation="MAVTYEKTFEIEIINELSASVYNRVLNYVLNHELNKNDSQLLEV
                     NLLNQLKLAKRVNLFDYSLEELQAVHEYWRSMNRYSKQVLNKEKVA"
     gene            complement(4147..4362)
                     /locus_tag="IV334_002611"
     CDS             complement(4147..4362)
                     /locus_tag="IV334_002611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000260238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-binding protein"
                     /protein_id="HAP8944629.1"
                     /translation="MIVGNLGAQKAKRNDTPISAKKDIMGDKTVRVRADLHHIIKIET
                     AKNGGNVKEVMDQALEEYIRKYLPDKL"
     gene            complement(4454..>4709)
                     /locus_tag="IV334_002612"
     CDS             complement(4454..>4709)
                     /locus_tag="IV334_002612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015543629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="chromosome partitioning protein ParA"
                     /protein_id="HAP8944630.1"
                     /translation="IGFVPYLVDTDSATIKSNLEELYKQHKEDNLVFQNIIKRSNKVS
                     TWSKNGITEHKGYDKKVLSMYKNVFFEMLERIIQLENEKE"
BASE COUNT         1445 a          796 c          865 g         1603 t
ORIGIN      
        1 tcttgggagt tttgagacta ctatatgcgt ttttttgttg atttaccaac atgtttagac
       61 attttcctct aggaaaaaat taactagcac cacgggttat cttttatata gaagatatcg
      121 ccgtatgtaa ggatttcagg gggcaaggca taggcagcgc gcttatcaat atatctatag
      181 aatgggcaaa gcataaaaac ttgcatggac taatgcttga aacccaggac aataacctta
      241 tagcttgtaa attctatcat aattgtggtt tcaaaatcgg ctccgtcgat actatgttat
      301 acgccaactt tgaaaacaac tttgaaaaag ctgttttctg gtatttaagg ttttagaatg
      361 caaggaacag tgaattggag ttcgtcttgt tataattagc ttcttggggt atctttaaat
      421 actgtagaaa agaggaagga aataataaat ggctaaaatg agaatatcac cggaattgaa
      481 aaaactgatc gaaaaatacc gctgcgtaaa agatacggaa ggaatgtctc ctgctaaggt
      541 atataagctg gtgggagaaa atgaaaacct atatttaaaa atgacggaca gccggtataa
      601 agggaccacc tatgatgtgg aacgggaaaa ggacatgatg ctatggctgg aaggaaagct
      661 gcctgttcca aaggtcctgc actttgaacg gcatgatggc tggagcaatc tgctcatgag
      721 tgaggccgat ggcgtccttt gctcggaaga gtatgaagat gaacaaagcc ctgaaaagat
      781 tatcgagctg tatgcggagt gcatcaggct ctttcactcc atcgacatat cggattgtcc
      841 ctatacgaat agcttagaca gccgcttagc cgaattggat tacttactga ataacgatct
      901 ggccgatgtg gattgcgaaa actgggaaga agacactcca tttaaagatc cgcgcgagct
      961 gtatgatttt ttaaagacgg aaaagcccga agaggaactt gtcttttccc acggcgacct
     1021 gggagacagc aacatctttg tgaaagatgg caaagtaagt ggctttattg atcttgggag
     1081 aagcggcagg gcggacaagt ggtatgacat tgccttctgc gtccggtcga tcagggagga
     1141 tatcggggaa gaacagtatg tcgagctatt ttttgactta ctggggatca agcctgattg
     1201 ggagaaaata aaatattata ttttactgga tgaattgttt tagtacctag atttagatgt
     1261 ctaaaaagct ttaactacaa gctttttaga catctaatct tttctgaagt acatccgcaa
     1321 ctgtccatac tctgatgttt tatatctttt ctaaaagttc gctagatagg ggtcccgagc
     1381 gcctacgagg aatttgtatc gataagaaat agatttaaaa atttcgctgt tattttgtac
     1441 atttaacttg acggtgacat ctctctattg tgagttatta gtggtacagt tttcaaccgt
     1501 tttaattata aaaaagtggt gcatttttaa attggcacaa acaggtaacg gttattgcag
     1561 gtgtatttct tatctatggg tttaacatgg attttatcat taaaatcatg agtattgtcc
     1621 gagagtgatt ggtcttgcgt atggttaacc ctaaagttat ggaaataaga cttagaagca
     1681 aacttaagag tgtgttgata gtgcattatc ttaaaatttt gtataatagg aattgaagtt
     1741 aaattagatg ctaaaaattt gtaattaaga aggagggatt cgtcatgttg gtattccaaa
     1801 tgcgttatgt agataaaaca tctactgttt tgaaacagac taaaaacagt gattacgcag
     1861 ataaataaat acgttagatt aattcctacc agtgactaat cttatgactt tttaaacaga
     1921 taactaaaat tacaaacaaa tcgtttaact tctgtatttg tttatagatg taatcacttc
     1981 aggagtgata acatgaacaa aaatataaaa tattctcaaa actttttaac gagtgaaaaa
     2041 gtactcaacc aaataataaa acaattgaat ttaaaagaaa ccgataccgt ttacgaaatt
     2101 ggaacaggta aagggcattt aacgacgaaa ctggctaaaa taagtaaaca ggtaacgtct
     2161 attgaattag acagtcatct attcaactta tcgtcagaaa aattaaaact gaatactcgt
     2221 gtcactttaa ttcaccaaga tattctacag tttcaattcc ctaacaaaca gaggtataaa
     2281 attgttggga atattcctta ccatttaagc acacaaatta ttaaaaaagt ggtttttgaa
     2341 agccgtgcgt ctgacatcta tctgattgtt gaagaaggat tctacaagcg taccttggat
     2401 attcaccgaa cactagggtt gctcttgcac actcaagtct cgattcagca attgcttaag
     2461 ctgccagcgg aatgctttca tcctaaacca aaagtaaaca gtgtcttaat aaaacttacc
     2521 cgccatacca cagatgttcc agataaatat tggaagctat atacgtactt tgtttcaaaa
     2581 tgggtcaatc gagaatatcg tcaactgttt actaaaaatc agtttcatca agcaatgaaa
     2641 cacgccaaag taaacaattt aagtaccgtt acttatgagc aagtattgtc tatttttaat
     2701 agttatctat tatttaacgg gaggaaataa ttctatgagt cgcttttgta aatttggaaa
     2761 gttacacgtt actaaaggga atgtagataa attattaggt atagggcacc tctaataact
     2821 accaattaat tcacctcaaa atgaaattag aaaaaaacag cgaaattttt aaatctattt
     2881 cttatcgata caaattcctc gtaggcgctc gggaccccaa catgaggaaa tgtttttata
     2941 aaaaaagagt agttaccgtt ttttggtaac tactcttttc cttttttaag gtttaaatac
     3001 ctggaagttt aggtgttttg ggtattggtg gtgtaggtgg ctgcaagctt tccaattttt
     3061 gttgaattgc tttaaattca ggtgtctctt ggtgtttatt tagaaccatt ttttgctcta
     3121 ttcgttctaa agtcggttga atttctttcc ctgatacttt acgattcaat tctttttcta
     3181 aggtttcttt tggactaata gaaggtgttt ccaaacttga atagagtttt actccttctc
     3241 tgttatatag ccttatatcg ctaaataagc ccgttttatg aagggtttct aaattggtcg
     3301 gtaagttttt gacaacaata tcatgcgctt gttttggtgt tgccctggct gtcattggat
     3361 catctgcata catggtttca tatcgttcaa ttgttcctaa atatgagttg attttaggta
     3421 ctgccatgac atacattttt gtttcataac ctttggcttg aagcattgtt gcggtttgaa
     3481 taggaacgtc tgttgttcgt cctgtacctt cgatcaccaa attataccct tgatcgctca
     3541 aacggcttat gatcgcttct gtcatgcgat tagaataagg ggtaacgtgt tttactacgt
     3601 ctttttcata aagtttcact agttcatcaa aattagggtg ctgttgttta aaggtatcat
     3661 tatcaatgac aataacattc ccttgtgttt cttcaaaaat tgctgatcgc aaactggttt
     3721 tccctgaccc tggttgccca ccaagtaaaa aagcggttgg cgattcaacc gctttttttc
     3781 cttgaatcaa ttcttctaaa ttatcattta agcgattctc aaattgtttg tcagtaaaat
     3841 tgactatatt tgccatatta agccactttc tctttattca aaacttgttt tgagtaacga
     3901 ttcattgacc gccaatactc atgaacggct tgtaattctt ctaaagaata atcaaaaaga
     3961 tttacacgtt ttgcaagctt taattggttt aataaattga cttccaataa ttgagagtca
     4021 tttttattta attcatggtt caaaacatag tttaatactc gattataaac gcttgccgat
     4081 aattcgttaa tgatctctat ttcaaatgtt ttttcatacg taactgccat ttcattttca
     4141 ctccttttaa agtttgtcag gtaaatattt ccgtatatat tcttctaagg cttgatccat
     4201 aacttctttt acgtttccgc cattctttgc tgtttcgatt tttatgatat ggtgcaagtc
     4261 agcacgaaca cgaaccgtct tatctcccat tatatctttt tttgcactga ttggtgtatc
     4321 atttcgtttt gctttttgtg cgcctaaatt tcccacaatc actcacttct ttctatttct
     4381 tcttactctt attttatcat caacaatcac aaatcacaag tgatttgtga ttaatcactt
     4441 gtgatttgtg attctattct ttttcgtttt ctaattgaat aattcgctca agcatttcaa
     4501 aaaatacgtt cttatacatg gataaaactt ttttgtcata gcctttatgc tctgtaatgc
     4561 cgtttttaga ccaggtactt actttattac ttcgcttgat aatattttgg aaaaccaagt
     4621 tatcttcttt atgttgctta tacagttctt ccaggtttga ttttatcgtt gcgctgtccg
     4681 tatcaactaa ataaggaaca aaaccgatc
//
LOCUS       DACDKM010000148         4639 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20834_2016
            SAMN12640370-rid9891663.denovo.148, whole genome shotgun sequence.
ACCESSION   DACDKM010000148 DACDKM010000000
VERSION     DACDKM010000148.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640370
            Sequence Read Archive: SRR10040891
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4639)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4639)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742835.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742835.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:04:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,012
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 29 of 75
            Pseudo Genes (incomplete)         :: 33 of 75
            Pseudo Genes (internal stop)      :: 19 of 75
            Pseudo Genes (multiple problems)  :: 5 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4639
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640370-rid9891663.denovo.148"
                     /isolate="E20834_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..269
                     /locus_tag="IV334_002613"
     CDS             <1..269
                     /locus_tag="IV334_002613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016179791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glutamate:gamma-aminobutyrate antiporter"
                     /protein_id="HAP8944631.1"
                     /translation="SVFALLISFVPPASIAASEHRTYQIILLISFVVTVLLPFIIYEV
                     HDKSAHTTIEEPTHVKAEDVNPAVYPAARGEHHVTKNDEHILKH"
     gene            282..1682
                     /locus_tag="IV334_002614"
     CDS             282..1682
                     /locus_tag="IV334_002614"
                     /EC_number="4.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003134257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate decarboxylase"
                     /protein_id="HAP8944632.1"
                     /translation="MLYGKDNQEEKNYLEPIFGSASEDVDLPKYKLNKESIEPRIAYQ
                     LVQDEMLDEGNARLNLATFCQTYMEPEAVKLMTQTLEKNAIDKSEYPRTTEIENRCVN
                     MIADLWHAPNNEKFMGTSTIGSSEACMLGGMAMKFAWRKRAEKLGLDIQAKKPNLVIS
                     SGYQVCWEKFCVYWDVELREVPMDEKHMSINLDTVMDYVDEYTIGIVGIMGITYTGRY
                     DDIKGLNDLVEAHNKQTDYKVYIHVDAASGGFYAPFTEPDLVWDFQLKNVISINSSGH
                     KYGLVYPGVGWVLWRDQQYLPEELVFKVSYLGGEMPTMAINFSHSAAQLIGQYYNFVR
                     YGFDGYRDIHQRTHDVAVYLAKEIEKTGIFEIINDGSELPVVCYKLKEDPNREWTLYD
                     LSDRLLMKGWQVPAYPLPKDLDQLIIQRLVVRADFGMNMAGDYVQDMNQAIEELNKAH
                     IVYHKKQDVKTYGFTH"
     gene            complement(1847..3287)
                     /locus_tag="IV334_002615"
                     /pseudo
     CDS             complement(1847..3287)
                     /locus_tag="IV334_002615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328084.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
     gene            3680..4273
                     /locus_tag="IV334_002616"
                     /pseudo
     CDS             3680..4273
                     /locus_tag="IV334_002616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328085.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ParB-like nuclease domain-containing protein"
     gene            complement(4257..>4639)
                     /locus_tag="IV334_002617"
     CDS             complement(4257..>4639)
                     /locus_tag="IV334_002617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HAP8944633.1"
                     /translation="FSRIKQPLTEVEIFHTDRGKEFDNQAIDELLTTFDINRSLSHKG
                     CPFDNAVAESTYKSLKVEFVYQYTFETLQQLDLELFDYVNWWNHLRLHGTLGYETPVG
                     YRNQRLAQRILDNELGCANASEAV"
BASE COUNT         1530 a          770 c          867 g         1472 t
ORIGIN      
        1 tatcagtgtt cgcattgctt atctcttttg taccaccagc ttcaattgca gctagtgagc
       61 accgaacata ccaaatcata ctacttatta gctttgtagt gactgttctt ttacctttta
      121 tcatttatga agtacatgat aaaagtgctc acacaactat tgaggaacct actcacgtga
      181 aagctgaaga cgttaatccc gctgtttatc cagctgctag aggagagcac cacgttacaa
      241 aaaatgatga acatatttta aaacattagg aggaattcat tatgttatac ggaaaagata
      301 atcaagaaga aaaaaactat ttggaaccaa tttttggctc tgcaagtgag gatgttgact
      361 tgcctaaata taagttaaac aaagaatcca ttgaaccacg aattgcttat caattagtac
      421 aagacgagat gttggatgaa ggaaatgcgc gattaaactt agctactttt tgtcaaacgt
      481 atatggaacc tgaagcagtg aaattgatga cccaaacgtt agaaaaaaat gcaattgata
      541 aatcagaata cccacgaaca acggaaattg aaaaccgctg tgtaaatatg attgctgatt
      601 tatggcatgc tccaaataat gaaaaattca tgggaacttc aacgatcggc tcttcagaag
      661 cctgcatgct gggtggtatg gccatgaaat ttgcttggcg taaacgtgct gaaaaattag
      721 gtcttgatat tcaagcaaaa aaacctaacc tggtgatctc ttctggttac caagtttgtt
      781 gggaaaaatt ctgtgtatat tgggatgtgg aactgagaga agtcccaatg gatgaaaaac
      841 atatgtcaat taatctagat actgtcatgg attatgtgga tgagtacaca attggtattg
      901 taggtattat gggtattact tacactggtc gttatgatga tatcaagggt ctgaatgatt
      961 tagttgaagc tcacaataaa caaactgact ataaagtata cattcatgtt gacgctgcat
     1021 cgggtggctt ttatgcacca tttactgaac ctgatctagt ttgggatttt caattgaaaa
     1081 atgttatctc aattaattct tcaggtcaca aatatggttt ggtatatcca ggtgtgggtt
     1141 gggtcttatg gcgtgaccaa caatacttac cagaagaatt agtatttaaa gttagttact
     1201 taggtggaga aatgccaact atggctatca acttctctca tagtgcagca caactaattg
     1261 ggcaatacta caactttgta cgctatggct ttgatggtta tcgtgatatt caccaaagaa
     1321 ctcatgatgt tgctgtttat ttagccaaag agatcgaaaa aactggtatt tttgaaatca
     1381 ttaatgatgg atcagaattg ccagttgtgt gctataagct gaaagaagat cccaatcgcg
     1441 aatggacact atatgattta tctgatcgtc tgttaatgaa gggatggcaa gtcccagcct
     1501 acccattacc taaagacttg gatcaattaa ttattcaacg cttagttgtt cgagcagact
     1561 ttggaatgaa catggctggt gattatgtac aagatatgaa ccaagcaatt gaagagttga
     1621 ataaagctca tattgtttat cataaaaaac aggatgtgaa aacgtacggc tttactcact
     1681 aaaattcttc agagaaatat aaaagaagtt aattaactaa taaaaggagt tgtgattacc
     1741 acacagctcc ttaaatctat ttgaataaat atatgatatt catttgttac actctcaatc
     1801 ttagctgttg ttctattcat cattactctt attgtaagta gttcagtcaa atttgcttgt
     1861 tcaattgatc gataacagta tgaattgttt gaaaagtaat cggatatata attgttttct
     1921 cactacctaa gccttcttgg tataaagaac tatcgcaaag tataatccgc tgagtacaaa
     1981 agaaagtgtc gtctactatt tctctaagtt gtttacaaat gatattacta atagaaaagt
     2041 gttctcccaa aatgtcttct aactgttgtc gatagtataa atacctaaag tctgacggag
     2101 ctactatgaa cacttcaatt ggagatttcc gtttatgctc atttaaaaga cttaaactaa
     2161 tggttagttt acgaatagaa ttctcattcc aacgcagaaa taaattgttt tgttttcgcc
     2221 attgttcaag aatttcatga acttctaagt acaaagcgcg ttgctcttca tttaacaagt
     2281 aagtcttatc aggttgaaat atctgaatat cagcccaaag tgagcggata aacgggagaa
     2341 gtgctttatc aaaaagtatc tgattttctt cagatatatt cagagataag ataagttttt
     2401 gactgagctc aatcaccaaa gggttatctg ctacatggtt ttgataaaca acgttcaagt
     2461 ctttttgtaa caattcaaag ttatcgcata gataatttct agagttgaat agaaagtaga
     2521 tataaaggca ttcatcttcg tcatgacata aagagaaaga aagttgctct aatcctttct
     2581 gtattagctg aaacaaggga gtatgtcggg caactctttt ttcttcctga ctaaaatata
     2641 ttgggttagt cttagatctc tgcaaaccga tagcgatacc taataaaata aattgttttt
     2701 caactgtagg gtaacttctt gaaaaaaaat gtttttctac ataatcaatc aattgttgac
     2761 cgctttgaat aattgattta tttagctgga tcaactctgt ccgatttgtc tcatagatta
     2821 tcgttagtaa caaacttcga taatcctttt caggaatttc aaaatggcca tcagcattta
     2881 agtactgcat ttgtttaaaa aaattaatca actcatttcg taagttgtaa actttgctta
     2941 acgaaatgaa ggtagtatca gctatattac tccaattgtg ttcccctgat aaataatgtc
     3001 ctagtacatt gaaaaagtca gagcgctgat agatgagttg agtgagtgcc aacctcgaaa
     3061 acgaagagtc gaatgcgact gtaaagctac ttctagattt ctccactgaa ataatttgct
     3121 gatggagtat gtcggtaatg cattccaagt cgttagtgac agacagtgag tgggcaaacg
     3181 ttcaattgat ctgctatttc gcgaatctga atggtccctt tttcgtgtaa ttgctcacaa
     3241 atgtaaactt gacgaaaaat acttttctcc agataggttt ccaacatagt actcctccta
     3301 gtcattttat acataactat tataactcta ccaatcggaa tagtcctgta atatattgaa
     3361 tcaaaaatat aaattataaa ataattagat ggggtatttg gtttaagtat cccttatata
     3421 gtaataaatt tataggtgtg attctcgaat taatacccga aaatgtgaga gaatttttat
     3481 ggatttttta ggatacaaat taattatttt tgcttttatc ctgattacca gtccagtact
     3541 gtcggtatcc cattttgcag caaaatcatg ttgataaagt gacaagaaga ctgatgagat
     3601 atattatcca aattgagaag ataattaatc ggattcttga gtatacccta ctttttaaga
     3661 aagtagggta tacaagcgta tgaataaaga cataactttc cctgtgatgg acgttcggct
     3721 tgttccaatt accaaaatac aggcgaacga ttataatccc aatcgagtag cgtctccgga
     3781 aatgaactta ttggagcttt ctatcattga agatggcttt actcaaccat tagtttgtta
     3841 ttacaaggaa aaagaagaga agtatatctt ggttgatgtg cttcataggt atcgtgttgc
     3901 taaggaaaga ctcaacttaa aagttgtacc ggtaacagtt attgataaac ccatataaga
     3961 acgtatggct tcaacaattc gacacaatcg tgcaagaggg acccatgaaa taagaggaat
     4021 ggaaattgtc atccaaagat tagtaaaaaa tggatggtca gacaagagaa tatccaaaga
     4081 attaggcatg gacattgaag aaatttttcg ctttaagcaa gtgtctggtg gaatggcaac
     4141 agtttttttg acaaatttta taaggtgcag aacttctttc cgtatgctat tccgattgtc
     4201 cttgacaatg agcctcctcg gatgtgacga tcttcggcag gagctaacag ttaaagttag
     4261 actgcctcgc tagcgttagc acatccgagc tcattatcaa ggattcgctg cgccaatctc
     4321 tggttacggt aaccaaccgg tgtctcgtag ccaagtgtac cgtgcaaccg aaggtggttc
     4381 caccaattta catagtcaaa taactccaaa tccaattgtt gtaaggtttc aaatgtgtat
     4441 tgatagacaa attctacttt caacgactta taagttgatt cagctacggc attatcaaaa
     4501 ggacagcctt tatgactcaa tgatcgattg atgtcaaaag ttgttaataa ttcatcaata
     4561 gcttggttat caaactcttt tccacgatca gtatgaaaaa tctcaacctc tgtcagaggt
     4621 tgtttgatac ggctaaatg
//
LOCUS       DACDKM010000149         4635 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20834_2016
            SAMN12640370-rid9891663.denovo.149, whole genome shotgun sequence.
ACCESSION   DACDKM010000149 DACDKM010000000
VERSION     DACDKM010000149.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640370
            Sequence Read Archive: SRR10040891
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4635)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4635)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742835.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742835.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:04:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,012
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 29 of 75
            Pseudo Genes (incomplete)         :: 33 of 75
            Pseudo Genes (internal stop)      :: 19 of 75
            Pseudo Genes (multiple problems)  :: 5 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4635
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640370-rid9891663.denovo.149"
                     /isolate="E20834_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            527..1438
                     /locus_tag="IV334_002618"
     CDS             527..1438
                     /locus_tag="IV334_002618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8944634.1"
                     /translation="MKYLLHGYFIDYSTRNSSTLAILCLLHELRVVTRKQLYKLLNFD
                     RSISKQGVTNALVRLYEKQLIDRKKYGTEMYYYLTKEGHQSIGGYYTLPKVPEYNLNH
                     HLQINNYLIKGMALAKDHPHLKRIISERRTVYETKDLNPKTKGRKFFVPDFIFRFRDK
                     ENHEINWSFEIELTLKTRRRYREAIFPKYIRELRTRPESHLIYVTPSGAIQEELHAFK
                     RIFFKRETEKDASQQELFERLHIFSALEFEEGLKALLENDPAVNWRTVEREKIYPNGA
                     VQSRLNLFEESTENEEKPKVKFTPEVL"
     gene            1925..2191
                     /locus_tag="IV334_002619"
     CDS             1925..2191
                     /locus_tag="IV334_002619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001245899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8944635.1"
                     /translation="MTTKNEQLKEQARKELQERGLIIEGSFEGDFETYIGCYARPINK
                     PTALDPMNEQEVKEQEKHAVNGFPQDFTEWYEWEINNGKLENFL"
     gene            2263..2604
                     /locus_tag="IV334_002620"
     CDS             2263..2604
                     /locus_tag="IV334_002620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8944636.1"
                     /translation="MAKNTPYQVGDIFYSSWGYEQTNVTFWQIVKLTEKTAWFQPIQK
                     KTVEHYSSMSGKVVPMKDKFIEYPLAKTKDSIVKKRIDPETYPDMLCGYRSWDTFHRY
                     KNQAVYESSYY"
     gene            2659..3078
                     /gene="ssb"
                     /locus_tag="IV334_002621"
     CDS             2659..3078
                     /gene="ssb"
                     /locus_tag="IV334_002621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HAP8944637.1"
                     /translation="MMNSVNLVGRLTKEVDLKYTPKGNATGTFILAVNRNYTNANGER
                     EADYIRCVIWRKAAETLAKYTGKGSLIGINGRLQTRSYQNQQGQTMYVTEVLVTDFYL
                     LESKEINEQRSKSMANNQNNSSIPGNEIQISDSYLPF"
     gene            3155..3451
                     /locus_tag="IV334_002622"
     CDS             3155..3451
                     /locus_tag="IV334_002622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8944638.1"
                     /translation="MKIIDSYDQRTMQYLGSFDYTDENIINYVASLPPYQSVRLVDFA
                     SDELVLTTMGNFLDYVGDKKWLEKILSKLIAKQTGEEPINAIQLFNRYEQENND"
     gene            3466..3897
                     /locus_tag="IV334_002623"
     CDS             3466..3897
                     /locus_tag="IV334_002623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8944639.1"
                     /translation="MKRLKRSIFIVLALIDFVGTGCEAIDVQKAPLSEIKQAITKQDE
                     EQPLNTQVEIVNQNKPSFSEEDFSLENGTRQFFSDLDRLNRVGVASALLHKSRMPTEE
                     RDRLYIKPTGWKQKKMKNGDYLYNRCHLIGYQRATSFPISM"
     gene            4079..4384
                     /locus_tag="IV334_002624"
     CDS             4079..4384
                     /locus_tag="IV334_002624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8944640.1"
                     /translation="MKLDMPGRYKLLRVRLFLIKLTNLRMYGSITDLYSKDIRLRMQR
                     TLSKSKNVGHEIRDGRLAVYLSRVGKTCLIDSDESGNATKRFTEEIEQLAELNIRYF"
BASE COUNT         1723 a          719 c          972 g         1221 t
ORIGIN      
        1 aaaacctaag cgggaagtct ccgaataaca taccaagcgc cgacagccaa cagataaaga
       61 atacactact aaaaatagta gtagcataaa atttaaaagg gaaaaaatca acccctaccc
      121 cagtcttggg gtctcaaaac taactccccc ctaaccccct ctttcagcct cttttgaaag
      181 agggggagaa tcaaccccac ggccaacgcc ccagtgttct tggggctgtc cagaacagaa
      241 acttgcgaga gagtgggggt aaaacaagtg tagaggaaac ttgaagactt gttcgaacag
      301 aaacttgcga gagagccgga gtaaaacaag tgtagaggaa acttgaagac ttgttcgaac
      361 agaaacttgc gagagagttg gagtaaaaca agtgtagagg aaacttgaag acttgttcga
      421 acacaaactt acaagagagg ggagtaaagc agttgcagag gaatcaggaa gatttgttcg
      481 aacagaaact tgcaagaaag cattattaaa ttgaaggtga tgaaatatga agtatttatt
      541 gcatggctat tttattgatt attctactcg caatagtagt actttagcga ttctatgttt
      601 gcttcatgaa ttgagagtgg ttactagaaa acagctatac aaactattga attttgacag
      661 atcaattagt aaacaaggag taacgaatgc tttagtgaga ttatatgaaa aacagttaat
      721 cgatcgaaaa aaatatggaa cagaaatgta ttattatttg acgaaagagg gacatcaaag
      781 cataggtggg tattatacgt tgccgaaagt tcctgaatat aatttgaatc accatttaca
      841 aattaacaat tatttgatta aaggaatggc attggcaaaa gaccacccac atttaaaacg
      901 gattatttca gaaagacgta ccgtctatga aacgaaggat ttaaatccta agacaaaagg
      961 aagaaaattt tttgtgccag attttatttt tcgttttcga gacaaagaaa atcatgagat
     1021 caattggtcc tttgaaattg agctaacgtt gaaaacacgt cgtcgctacc gtgaagcgat
     1081 ttttccgaaa tatattcgtg agttgcgaac acgtcctgaa tcacatttaa tttatgtgac
     1141 acctagtggc gcaattcaag aagaacttca tgcgtttaag cgtatttttt tcaagcgcga
     1201 aactgaaaaa gatgcgagtc aacaagaatt gtttgagcgg ttgcatattt tttcagcttt
     1261 agagtttgaa gagggattaa aagcattatt agagaatgat ccagcagtca attggcggac
     1321 agtagaacga gaaaaaattt atccgaatgg cgctgttcaa agtcgtttga atttatttga
     1381 agaatcaact gaaaatgagg aaaaaccaaa agtgaagttt acgcctgaag tattgtaaaa
     1441 gagggtgttc gcttcgctct ttttttgtgc cagtttgctg gcaccgttca gacgcaagcg
     1501 tctgaaccgg ctcgttttta ggaaggacta acttgtttga tttatccgtt gtcaaagaag
     1561 tggacgttcg agagtcccta atcgtatagc agtcattcgg cgagtataac ctaaaacata
     1621 acctgccaat tgccaagggt aaaatgcacg ctgcgcgccc ttggcaatcg tcagaacatg
     1681 ttttcgagcc ggttatgccg aacgaccgct atacgaaaag ggcctctaaa cagaagtcca
     1741 ctttgatcct tgacaactga aacccaaaat caaacaagca ggccttcgtc taaaaacgag
     1801 ccgaatgaag accttggggg ttgcaccccc aaacccccgt gcccccgaca cactaccgaa
     1861 aaggtggggg tgggtggcga aaaaatcaaa taaaacattc tattcaagaa aggaagaaac
     1921 aaccatgaca actaaaaacg agcaactgaa agaacaggca agaaaagagt tgcaagaaag
     1981 aggtttgatt attgagggga gctttgaagg agattttgaa acctatattg gttgttatgc
     2041 aagaccgata aacaaaccta cagccttaga cccaatgaac gaacaagaag taaaagaaca
     2101 agaaaaacat gcagtgaatg gttttccgca agatttcact gaatggtatg agtgggaaat
     2161 taacaacggg aagcttgaaa acttcttata aaagtcagat gacgtgcaaa gagaatgatt
     2221 ctctttgtac ggtaaaaata taaagaaaga aggaaaaaga aaatggcaaa aaacacacct
     2281 tatcaagtag gagacatttt ttatagtagt tggggctacg aacaaacgaa cgtgactttt
     2341 tggcaaattg tgaagttaac agaaaaaaca gcatggtttc agcctattca aaagaaaacg
     2401 gtcgagcatt attcgtccat gagtggaaaa gttgttccaa tgaaagacaa gtttatagag
     2461 tatcctttag caaaaactaa agacagtatt gtaaaaaaac gaattgatcc agaaacctat
     2521 cctgatatgt tatgtggtta tcgtagttgg gacacgtttc atagatataa aaatcaagct
     2581 gtttatgaaa gtagttatta ttaaggggat agtttcccct ttcaaaaata ataaatagaa
     2641 gaatggagag aaaaaatcat gatgaattca gtgaatttag taggacggtt aacaaaagaa
     2701 gtggatttaa aatacacacc aaaaggcaat gcgacaggca catttatttt agctgttaat
     2761 cgaaattata cgaatgcaaa cggcgagcgt gaagcagatt atatccgctg tgtcatttgg
     2821 cggaaagctg cggaaacttt agcaaaatat actggaaaag gaagtttgat tggcattaat
     2881 ggacgtcttc aaacgaggag ctatcagaat cagcaaggac agaccatgta tgttacggaa
     2941 gttctagtga cagatttcta tttgttagag agtaaagaaa tcaatgagca acggtcaaaa
     3001 tcaatggcga ataatcaaaa taattcttca attccaggaa atgagattca aatttcagat
     3061 agttatttgc ccttttaagt gaaaaataat tttgttctat acctgatttc ttcaggtata
     3121 gaacaaaaaa taaatagaga tgaggcgatt ttttatgaaa atcattgaca gttacgatca
     3181 acgaaccatg caatatttgg gctcgtttga ttatacagat gaaaatatta ttaattatgt
     3241 tgcaagttta cctccttatc aaagcgttcg cttggtagat tttgcaagtg atgaattggt
     3301 attaacaacg atgggcaact ttttagatta tgtcggagac aaaaaatggt tggaaaaaat
     3361 tttgtctaaa ttgattgcga aacaaacagg agaagaaccc ataaatgcga ttcaattgtt
     3421 caatcgatat gaacaagaaa acaatgatta aagaggagag aaaaaatgaa aagattaaaa
     3481 cgaagtattt ttattgtgct agcattgatt gattttgttg ggacaggttg tgaagcaatt
     3541 gacgttcaaa aagctccctt atcggaaata aaacaagcaa taacaaaaca agatgaagaa
     3601 caaccgctaa atacacaagt agaaatagtg aatcaaaata aaccgagttt ttctgaagaa
     3661 gatttttcgt tagaaaatgg aaccaggcaa tttttttcag atttggatcg tttaaatcgg
     3721 gtaggtgtag caagtgcact tttgcataaa agcaggatgc caacagaaga acgagaccga
     3781 ttatacatta agccaactgg ctggaaacaa aagaaaatga agaatggcga ttatctttat
     3841 aatcgttgtc atttgattgg ctatcaacgt gcgacaagtt tccccataag tatgtaatac
     3901 gaaacgggag gattaatctt aagattaatc acaactaacc tatttgacag ttggaatgaa
     3961 agctatcatg tctgctgaat caactggtct gatactccta catgcaataa agtctactag
     4021 gaagttattg tatgaagcta ggtaaaggcg gcagaacaat ttctaagtgt ggaatcatat
     4081 gaagttggat atgccgggac gctataaatt actcagggtg agactgtttt taattaaact
     4141 gacgaacttg cgaatgtacg ggtctataac cgacttatat tcgaaagata taagattgcg
     4201 aatgcaaaga acattatcga aaagtaagaa tgttggtcat gaaattcgag atggaagact
     4261 agcggtgtat ctgtcaagag ttggtaaaac atgtttgatt gattcagatg agagtgggaa
     4321 tgcgacaaaa cgttttacag aagaaataga gcaactagca gaactgaata tacgatattt
     4381 ttaaatactt tagcggagaa taaaaaaatc ctctacagaa aactacggag ggcaacatgt
     4441 gctattgttt caacaataca tttttaattt ttaccataac aaaaaagtac ttgaaaaaaa
     4501 acagaaatat gttaaattaa aaatgaaaaa atggaagcgg ttacttattc ttttgaatct
     4561 aagataaaaa ttcaaaagaa taacataacc caaatatgat tatacttgag attctaaata
     4621 tttatcgaaa aggaa
//
LOCUS       DACDKM010000150         4450 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20834_2016
            SAMN12640370-rid9891663.denovo.150, whole genome shotgun sequence.
ACCESSION   DACDKM010000150 DACDKM010000000
VERSION     DACDKM010000150.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640370
            Sequence Read Archive: SRR10040891
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4450)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4450)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742835.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742835.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:04:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,012
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 29 of 75
            Pseudo Genes (incomplete)         :: 33 of 75
            Pseudo Genes (internal stop)      :: 19 of 75
            Pseudo Genes (multiple problems)  :: 5 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4450
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640370-rid9891663.denovo.150"
                     /isolate="E20834_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(<1..721)
                     /locus_tag="IV334_002625"
     CDS             complement(<1..721)
                     /locus_tag="IV334_002625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HAP8944641.1"
                     /translation="MNSWIFCIDSDGCVMDTMTYKHNLFFGPIAAEIFDVHHREKFLQ
                     DWNNINLYSETRGINRFLGLVKTLEKNDVPSINRLKHWVSVTKELSNPSLKREIEYSQ
                     SEDLLKALKWSEEVNRQISQAKGLDKPFLSARDTIKALKKYGKIIIVSSANKEAIQEE
                     WERHELLSLVDELCCQDKGKKEDIIRSVIENGCDLDKILMIGDSPGDLEAANKNKVFF
                     YPILVNKEKESWENLRTDILFK"
     gene            complement(732..2108)
                     /locus_tag="IV334_002626"
     CDS             complement(732..2108)
                     /locus_tag="IV334_002626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP8944642.1"
                     /translation="MERKFGLRDKIGYMFGDFGNDFTFIFASSFLMIFYTKVLGISGA
                     TVGTLFLVARFIDAVTDITMGRIVDGSKPSKTGKFKPWILRMSGPVALASFLMYQTSM
                     ASASMSIKVIYMYVTYILWGSIFYTSINIPYGSMASAITSVAEERTALSTFRSIGATL
                     AGMFIGVLTPIFIYTRDSSGAQIVRGGSTFTIVAGVFSVCAVICYVICYKLTVERVKS
                     DESQLKVSLLKSLKGLAKNRALLSIIAAAIFLLLAQLLTMSMNNYVFPDYYNNAQGIV
                     LMNFINPILMLVLVSPVSLIFSKKYGKKELASVGMFFSAIVYLLLFLIKPSNMYVFLV
                     LTSVGYMGLGIFNTVIWANITDVIDDQEVKSGQREDGTIYAVYSFARKIGQALAGGVG
                     GWTLSIIGYDSLAKVQSGEVLQKLYNSSTLIPAVCFFVVGLILFFVYPLSKEKVLENY
                     NLLNQKHN"
     gene            complement(2237..2884)
                     /locus_tag="IV334_002627"
     CDS             complement(2237..2884)
                     /locus_tag="IV334_002627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HAP8944643.1"
                     /translation="MKKVEILSKLEAAGVIAVVRGKNKDEAIKAINAITAGGIKGIEL
                     TFTVPNAEEIIKEIAKKYADQKDVIIGAGTVLDAFTARIAIMAGAEYIVSPSFDRQTA
                     EICNLYQIPYLPGCMTITEIKEALTAGVEIIKLFPGSAFGPSVVKTYQAPLPYVNIMP
                     TGGVSLDNLEEWFEAGVVAVGVGGNLLAPVEKGDFEEVTRIASKYIDKFYEIKNY"
     gene            complement(2968..4395)
                     /gene="uxaC"
                     /locus_tag="IV334_002628"
     CDS             complement(2968..4395)
                     /gene="uxaC"
                     /locus_tag="IV334_002628"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HAP8944644.1"
                     /translation="MFLDKNFLLENEIAKRLFHKFAEHQPIIDYHCHLDPACLLENKQ
                     YKNLTQVWLNDKGAGDHYKWRLMRANGIEEELISGAGNDYEKFLAFVKTVEKAPGNPI
                     FEWSHLELQRYFDIDLTISEKNAPEIWRRANEKIQTDSYRPKELLKMMDVKAICTTDD
                     PTDDLRYHTELSKQDNSFKVLPTFRPDKVWSIKSSGFSNYILKLSQAAGIPIKNFNGL
                     KQALSQRVDYFHQIGCRLADHGLNTYFYEIADEKILDNLLIKALAGKTDFHLSEIAQF
                     VTGIQLHLMSEYTRYGWTMQMHLNALRNNSSILKEQIGVDVGGDSMGDQASLTQNVVA
                     LFDTAEKSGCLPKTILYSLNPTDWLPLATAMQSFQGGMIQKIQLGCAWWFNDTAEGMR
                     QQLTTMAQQSLLSNFTGMLTDSRSFLSYPRHEYFRRVLCQLLSEWVKAGRIPDDEMYL
                     SQIVENICYRNAYNYFNFFEIESRK"
BASE COUNT         1437 a          903 c          568 g         1542 t
ORIGIN      
        1 atttaaataa aatatcagtt cttaagtttt cccatgattc tttttcttta ttaaccaaaa
       61 taggataaaa gaaaacttta ttcttatttg cagcttctag atcaccagga gaatcaccaa
      121 tcattaatat cttatccaaa tcacaaccat tttcaattac acttcgaata atgtcttctt
      181 tttttccttt atcttgacaa cacagttcat caactaagga taataattca tgtctttccc
      241 attcttcttg aatagcttcc ttattagctg agcttacaat aattatcttc ccatattttt
      301 tcagagcttt tatagtatct ctagcactta aaaagggttt atctaatcct ttagcttgac
      361 taatctgtcg attgacttct tccgaccatt ttaaagcttt taataaatcc tctgactgag
      421 agtattctat ttccctcttc aaagatggat tagacaattc ttttgttaca gatacccagt
      481 gctttaatcg attaatcgat ggtacatcat ttttttctaa tgttttaact aaacctaaaa
      541 atctgtttat tccacgagtc tctgaatata aattaatatt attccaatcc tgtaggaatt
      601 tttccctatg atgaacatca aaaatctctg cagcaattgg cccgaaaaag agattgtgtt
      661 tataagtcat tgtatccatg acacaaccat ctgaatcaat acaaaatatc caactgttca
      721 tgttttttct cctagttatg tttttgattt agcaaattat aattttctaa tactttttct
      781 ttagataatg gatatacgaa aaataaaatc aatcctacaa caaagaaaca aactgctgga
      841 atcaacgttg aagagttata cagcttttgt aaaacttctc cactttgtac ttttgctaac
      901 gaatcatacc ctattataga aagagtccaa cctccgacac cgccagctaa agcttgtcca
      961 atttttcggg caaaagaata tactgcataa atagttccat cttctctttg gccagatttc
     1021 acttcttgat catcgattac atcagtaata ttcgcccaaa tgactgtatt aaaaatacct
     1081 aaccccatat atccaacaga agtaagaact aagaatacat acatattact aggctttatc
     1141 aaaaataaaa gtaaatatac aatagcagag aagaacatcc ctactgacgc caattctttt
     1201 ttaccgtatt ttttactgaa aattaaactt acaggtgaaa caagtaccaa catcaaaata
     1261 ggattgataa agttcattaa aactattccc tgtgcattat tataataatc tggaaataca
     1321 taattgttca tactcattgt taaaagctga gcaagcaaca aaaaaatagc tgcagctatt
     1381 atagaaagca acgctctatt tttagctaat ccttttaaag atttcaacaa tgaaactttt
     1441 aactgcgatt catcactttt aacccgttct acagttaatt tataacatat tacgtaacaa
     1501 ataactgcac acactgaaaa tactcctgcc acaattgtaa aagttgatcc accacgtaca
     1561 atttgagctc cagaactatc tcttgtataa ataaaaattg gtgttaacac gccaatgaac
     1621 atacctgcta aggtagctcc tatactacga aatgttgata acgctgtcct ttcttctgca
     1681 acagaagtga tagctgatgc cattgaaccg taaggaatat taatagatgt ataaaaaata
     1741 cttccccata gtatatatgt tacatacata tatataactt taatactcat agaagctgat
     1801 gccattgatg tttgatacat taaaaaactt gctaaagcga ctggaccact catccgtaaa
     1861 atccacggtt taaatttgcc agttttagat ggttttgatc catccacaat acgccccatt
     1921 gttatgtctg taactgcatc aataaatctt gctactaaaa ataatgttcc tacggtagct
     1981 ccgcttatcc ccaatacttt tgtataaaaa atcattaaaa aagaactagc aaaaatgaaa
     2041 gtgaaatcat tcccaaaatc tccaaacata tatcctattt tgtctcttag accaaatttt
     2101 ctttccatat aatttgcgct ccttcctatt gttaagcgtt ttcattctgt tgaactgcca
     2161 acttacgtac tatactctga agtataaaga gttagtattt attttttaca ttttatagta
     2221 ttatttattt tttctcctaa taattcttta tctcataaaa cttatctata tatttactag
     2281 ctattcgagt tacttcttca aaatcacctt tttcaacagg agccaacaaa ttcccaccta
     2341 caccaactgc aacaacgcct gcttcaaacc actcttctaa gttatctaga cttactccgc
     2401 cagtaggcat tatatttaca tatggtaatg gagcttgata cgtttttaca acagatggac
     2461 caaaagcaga acctggaaat agtttaatga tttctactcc agcggtaagt gcctccttta
     2521 tctcagtaat tgtcatacaa cctggtaaat aaggaatttg atataaatta caaatctctg
     2581 ctgtttgtcg atcaaaagat ggacttacga tatattctgc tccagccata attgcaatac
     2641 gagcagtaaa cgcatctaaa actgtaccgg ctccaattat tacatctttt tgatcagcat
     2701 attttttagc aatttctttg attatttctt cagcattcgg aactgtaaaa gttagttcaa
     2761 tccctttaat tccacccgca gtaattgcat ttattgcttt aatggcctca tctttatttt
     2821 ttccacgtac aaccgcaatg actccagccg cttctaactt acttaaaatt tctacttttt
     2881 tcatatctat ctccttcttt tatggataca tactctagct agttgtacta acttacaata
     2941 aataaattta agcttttatt ttttgtatta ttttctactt tcgatttcaa aaaaattaaa
     3001 ataattatac gcattacgat aacaaatatt ctccactatc tgactaagat acatttcatc
     3061 atctggaatt cgacctgctt taacccactc gcttagtaat tgacataata ctcgacggaa
     3121 atactcatgg cgaggatagc ttagaaaact tcgagaatca gtcaacatac ctgtaaaatt
     3181 cgacaacaaa ctttgttgcg ccatcgtagt caactgttga cgcattcctt cagctgtatc
     3241 attgaaccac caagcacatc ccaattggat tttttgaatc ataccccctt ggaagctttg
     3301 cattgctgtt gctagtggaa gccaatcggt tggatttaat gaatataaaa ttgtttttgg
     3361 taagcaccca ctcttttcag cagtgtcgaa caatgctaca acattttgtg tcaaactagc
     3421 ttgatccccc atcgaatcac caccgacatc tactccaatt tgttccttta atatacttga
     3481 attatttctt aaagcattta aatgcatttg catcgtccaa ccatatcttg tatactcaga
     3541 catcaaatgt aattgaatac ctgtaacaaa ttgtgcaatt tctgataaat ggaaatccgt
     3601 ttttcctgct aaagctttta ttaacagatt gtcgagtatt ttttcatctg ctatttcata
     3661 aaaataagta ttcaaaccgt gatctgctaa tcgacagcca atttgatgaa aataatccac
     3721 tcgttggctc aatgcctgct tcaacccatt aaagtttttg attggtatac ccgctgcttg
     3781 actaagtttt aaaatatagt tcgaaaatcc tgatgatttg atactccaga ccttatctgg
     3841 acggaaagta ggtaatacct tgaaagaatt atcctgttta gaaagctcag tatgatatcg
     3901 caagtcatct gtaggatcat ccgttgtaca aatggctttc acatccatca tcttcagcaa
     3961 ttcttttggg cgataggaat ccgtttggat tttttcgttg gcacgtcgcc aaatttctgg
     4021 tgcattcttc tcagaaattg tcaaatcaat atcaaagtaa cgttgtaatt ccaaatgaga
     4081 ccattcaaaa ataggattac ctggcgcctt ctccactgtt tttacaaaag ctaaaaattt
     4141 ttcataatca ttacctgcac cactgatcaa ctcttcttct atcccattag ctcgcatcaa
     4201 tcgccattta taatgatctc cagctccttt atcatttaac catacctgag tcaaattctt
     4261 gtattgctta ttttctagca aacatgctgg gtctaaatga caatgataat cgatgattgg
     4321 ttggtgttct gcaaatttat gaaaaagtct tttagctatt tcattctcta ataaaaagtt
     4381 cttatctaaa aacatattta agcactcctt tccttccctt agtttttaga agagaaaatt
     4441 tttcttctaa
//
LOCUS       DACDKM010000151         4425 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20834_2016
            SAMN12640370-rid9891663.denovo.151, whole genome shotgun sequence.
ACCESSION   DACDKM010000151 DACDKM010000000
VERSION     DACDKM010000151.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640370
            Sequence Read Archive: SRR10040891
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4425)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4425)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742835.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742835.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:04:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,012
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 29 of 75
            Pseudo Genes (incomplete)         :: 33 of 75
            Pseudo Genes (internal stop)      :: 19 of 75
            Pseudo Genes (multiple problems)  :: 5 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4425
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640370-rid9891663.denovo.151"
                     /isolate="E20834_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            690..1658
                     /locus_tag="IV334_002629"
     CDS             690..1658
                     /locus_tag="IV334_002629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HAP8944645.1"
                     /translation="MDVAKTVNAIVEKNDGEISGIYLVACGGSLVDMYVSDYFLKAEA
                     SKCITGLYTANEFNHVTPKQLDDKSVVILCSHGGNTAETVKAGEVAQARGAVTVGLTH
                     NKTADLLKVSDHSFLYEWGDDAKIVNNPMAIILDLTVSLVNVTETFEHYGKFREGMKV
                     IDTVIENGIKQVQDRIKVFADKYKDETMFYILGSGPSYGHAYGFSICSLMEMQWLDST
                     SVHSGEFFHGPFEVTDKDTNFILLMSIGRTRALDERAKRFLDKYAERVEIVDAKELGL
                     DMISDEVSEFFNPILFYSILSEYRSALADVRNHDLDVRRYMGKVEY"
     gene            1669..2484
                     /locus_tag="IV334_002630"
     CDS             1669..2484
                     /locus_tag="IV334_002630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 6-kinase"
                     /protein_id="HAP8944646.1"
                     /translation="MKVLGLGDNVVDKYENLGIMYPGGNSLNFAVFAKKLGHESSFMG
                     VFGSDEEAAHVLSAIKEIGLDNSHSRFEKGENGCARVKIDEGDRIFLGSNSGGVTREY
                     PIQLTEEDREYLNQFELIHMGLYSHVNHLLEELQNVSGKISYDFSDDFTEEEVQRAID
                     KVDFGFFSIEDFSDEEVKQFLMKHFCERNDVLIATRGEKTAIGYDGAKFYEVNPVLEE
                     PVDTMAAGDSFLTAFLLYFLEGDDIVTAMKKGNEFAGKSCLVDGSFGFGIHYD"
     gene            2552..3262
                     /locus_tag="IV334_002631"
     CDS             2552..3262
                     /locus_tag="IV334_002631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAP8944647.1"
                     /translation="MLDTNAQLPLYLQLKEILKNKINTGEYPENTKIPAESELIESYE
                     VSRITVRKAVEELVEEGYLLKKQGKGTFVNRHKVFRKIEYIMGFSESCKSNGFTPTSI
                     VLERKIIPATEGMAKKLDIAQGDDVLYIQRKRMADNIPIMLENNYFSYERYSFLMEEE
                     LTDSLYEVLKVQGTLAINPGETALEIVTADDQIAKIMEVAVGTPFFFMDTLINDQNNI
                     PIHVGNQYYLGEVYRFYL"
     gene            3298..>4425
                     /locus_tag="IV334_002632"
     CDS             3298..>4425
                     /locus_tag="IV334_002632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HAP8944648.1"
                     /translation="MKQILRSKMIFDGEQPPFKGYIVINEGIIEKVENNWNYSDLLNE
                     NTELIAYDDQFVMPAIHDNHVFFSGYLAMNAGIDLSDTSSANEAVERIKAVMNHKKAD
                     EPIYAHGWDAALWKTDPSEELLNEINHFGPITAIDKNRSHCWMNAIAKQTYGFTEKET
                     SAESRVLLIKEMLANKKLLAEVYAEFEALLLSKGVISMKEIVFDDAEFLSNIPQRKIL
                     SNLYVQAVQEPLQHELLYEYKEKVFPEKVRFGGVKIMVDGVVADRSGDIYGVYQSGVA
                     SPEIDYEAIKAEVAAFNEVEIPCCLTTEGDKAGYKAASILVEFGKRLPEGVFNSISDL
                     EMVTQKTAEKMNEGQVVAEVYPQILGLNQTLEEAYMSTVIKN"
BASE COUNT         1490 a          661 c          921 g         1353 t
ORIGIN      
        1 atacaaataa ttcaccaaaa aggtgagtaa tgccattttt gaaaattgag cttgttttat
       61 gtctacatct gtatccgtat ataatccaaa ggattataaa ctcagaagag tctttatttt
      121 ttctgaatat aactactgta tcaaaaacaa tcgattatga gacaaaacaa aatgacctct
      181 tttatttaag aaaggaggtc attttttgta tcataaattg tattaaaatc aatgtatcac
      241 tttttaatca gttttaagac cataaattgc gtactaaaag aaattctgca attcaaaata
      301 aagcatttta agccattttc aaatttaagt gcacccatct acccgtcaaa tcactttatt
      361 ttccactcca gtagtttcct aattcaactg tgtgcaaaaa cgatttcttc taaacaaatc
      421 aagacaaact tataaacctt catcccagca ccataattat ttaaatcata ggcatttttc
      481 gaaattagta tatgtactca agtttcactg cagattattt atatttttta gagaaataaa
      541 tttttcctat tgacaaacac aaagaaagcg gatacaatgc aattatgtca catgacgtta
      601 tatgacgtta tgtgatatct ttacatttaa tcagttactt ttattttgga taaaattaac
      661 atttttaaaa ttttaaggag gaaatgttga tggatgtagc aaaaacggtt aacgcaattg
      721 tagaaaaaaa tgatggagag atttctggaa tttatttggt ggcttgtggc ggttcgctgg
      781 tagatatgta tgtctcagat tattttctta aagccgaagc atcgaagtgt attacaggct
      841 tatatacagc gaacgaattt aaccatgtaa ccccaaagca attagatgat aaatctgttg
      901 tcattctatg ttctcatgga ggaaatacag cagaaacggt aaaagcagga gaagtcgctc
      961 aagctagagg agctgtgact gttggtctga ctcataataa aactgcggat ttattaaagg
     1021 tttctgatca tagtttcctt tacgaatggg gagatgatgc aaaaatagtc aataatccta
     1081 tggccatcat tcttgattta actgtatctt tggtcaatgt taccgaaaca tttgaacatt
     1141 acggtaaatt cagagaaggc atgaaggtta tcgataccgt aattgaaaat ggcattaagc
     1201 aggtgcagga tcgtattaaa gtgtttgctg ataagtacaa agatgaaaca atgttttata
     1261 tcttaggtag tggaccatca tatggacatg cttatggttt ttcaatttgc tctttgatgg
     1321 aaatgcaatg gcttgattca acttctgttc attctggaga attcttccat ggaccttttg
     1381 aagtaacaga caaagatacg aattttattc tcttgatgag cattggacgc actcgtgctt
     1441 tagatgaacg ggcaaaacga ttccttgata aatatgcaga aagagtagaa atcgttgatg
     1501 caaaggaact aggattggat atgatatcag atgaagtttc tgaattcttc aatcccattt
     1561 tattctacag tattctttca gaatatcgtt cagctttagc agatgtacgc aatcatgatt
     1621 tagatgttcg tcgctatatg gggaaagtgg agtactagga ggattaaaat gaaggtatta
     1681 ggcttaggtg ataacgtcgt cgataaatat gaaaatctgg gaattatgta tcctggagga
     1741 aattcattga attttgcagt ttttgcaaaa aaactgggac atgaaagtag ctttatggga
     1801 gtttttggct cagatgagga ggcagctcat gttttatcag caataaaaga aataggactg
     1861 gacaacagtc actctcgctt tgaaaaaggc gagaatggct gtgccagagt gaagattgat
     1921 gagggcgacc ggattttcct tggcagcaat tcgggaggtg ttactcgtga atacccgatt
     1981 cagcttactg aagaagacag agaatattta aaccagtttg aattgattca tatgggatta
     2041 tatagccatg tcaatcatct attagaagag ttacaaaatg tttctggaaa gatttcttat
     2101 gatttttctg acgactttac ggaagaagag gttcagcggg caattgataa agttgatttt
     2161 ggcttctttt ctatagaaga cttttccgat gaagaagtaa agcagttttt gatgaagcat
     2221 ttttgtgagc gaaatgatgt attgattgca acccgaggag aaaaaacggc tattggttat
     2281 gatggagcaa agttttatga agtgaacccc gtgctagaag aaccagttga tacaatggca
     2341 gcgggagatt ctttcttaac agctttccta ctttattttc tcgaagggga cgatatagtc
     2401 accgcgatga aaaaggggaa tgaatttgct gggaagtctt gtctggttga cggttcgttc
     2461 ggctttggta ttcattacga ttgagcttgt tttaaattcc tgcatttgat aacctaaaga
     2521 aagaaatccc ggtaaagtgg aggttaaaaa aatgctggat acaaatgcac aattaccatt
     2581 gtacttacaa ttaaaagaaa tattaaaaaa taaaatcaat acaggggagt atcccgagaa
     2641 tacgaaaatt cccgctgaat cagagctgat agaaagttat gaggtcagcc gaatcacagt
     2701 gagaaaagca gtagaagaac tggtagaaga agggtaccta cttaaaaaac aagggaaagg
     2761 aacttttgtc aaccggcata aagtttttcg taaaattgaa tatattatgg gtttcagtga
     2821 gagttgtaaa tctaatggtt ttacaccgac tagcattgtt ttggaacgca agatcattcc
     2881 agcaactgaa gggatggcaa aaaaactcga tatcgctcaa ggtgatgatg ttttatatat
     2941 ccagagaaag cgaatggcgg ataacatccc cattatgtta gaaaacaatt atttttctta
     3001 tgaaagatat agtttcttaa tggaggaaga gctgactgat tctttgtatg aagtacttaa
     3061 agtccaaggt accttagcca tcaatcctgg ggaaactgca cttgaaattg tcacagctga
     3121 tgaccaaatt gccaaaataa tggaagtagc tgttgggacg ccctttttct ttatggatac
     3181 tttaatcaac gatcaaaaca acataccaat tcatgtaggt aatcaatatt acctgggaga
     3241 agtatatcgt ttttacttat gatttcctgt ttggatttca aggagtagat tttatttatg
     3301 aagcaaatat tgcggtcaaa aatgattttt gatggagagc agccaccttt caaaggttat
     3361 attgtaatca atgaagggat cattgaaaag gtggaaaaca actggaatta cagtgatcta
     3421 ttaaatgaaa acacggaatt aattgcttat gatgatcaat tcgttatgcc agcaattcat
     3481 gataatcatg tatttttcag tggttatttg gctatgaatg ctggcataga tttaagcgat
     3541 acatcttcag ctaacgaggc tgtagagcgt attaaagctg taatgaacca taaaaaagca
     3601 gatgaaccaa tttatgctca tggctgggat gctgccctgt ggaaaactga cccatcagaa
     3661 gagctattga atgaaataaa ccactttggt ccaatcacag cgatcgacaa aaatcgcagc
     3721 cattgttgga tgaatgctat tgctaagcag acttatggtt ttacagaaaa agaaaccagt
     3781 gctgaaagtc gagtattatt aattaaggaa atgctagcaa acaaaaagct tttagcagag
     3841 gtttacgctg aatttgaagc attactgctt tcaaaaggtg ttatatcaat gaaagaaatc
     3901 gtttttgatg acgcagaatt tctttctaat atacctcaga ggaaaatact tagtaaccta
     3961 tatgtgcagg cagtccaaga acctctacag catgaacttc tatatgagta taaggaaaaa
     4021 gttttccctg aaaaggtccg ctttggtgga gtgaagatca tggtagatgg tgttgttgcg
     4081 gatagaagtg gagatattta cggtgtttat cagtcaggag tagcttcacc cgagattgac
     4141 tatgaggcaa ttaaagcaga agttgctgca ttcaatgaag tagagattcc ttgctgtttg
     4201 acaacggaag gagataaagc gggttataaa gctgcgtcaa ttcttgtcga atttggaaag
     4261 cggttacctg aaggcgtttt taattcgatt agtgatttgg aaatggttac tcaaaagaca
     4321 gcagaaaaaa tgaacgaagg acaagtagta gcagaggtat atccacaaat tctcggcttg
     4381 aatcaaacgt tagaagaggc ttacatgtca actgttataa aaaat
//
LOCUS       DACDKM010000152         4310 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20834_2016
            SAMN12640370-rid9891663.denovo.153, whole genome shotgun sequence.
ACCESSION   DACDKM010000152 DACDKM010000000
VERSION     DACDKM010000152.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640370
            Sequence Read Archive: SRR10040891
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4310)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4310)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742835.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742835.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:04:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,012
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 29 of 75
            Pseudo Genes (incomplete)         :: 33 of 75
            Pseudo Genes (internal stop)      :: 19 of 75
            Pseudo Genes (multiple problems)  :: 5 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4310
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640370-rid9891663.denovo.153"
                     /isolate="E20834_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(65..1285)
                     /locus_tag="IV334_002633"
     CDS             complement(65..1285)
                     /locus_tag="IV334_002633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015509263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HAP8944649.1"
                     /translation="MKLIGIVGSAAELSYNRKLLTFIQKQYHELFNLEIIEIKDLPLF
                     NQDDDQTNSPAIQQLNRKILMADGVIIATPEHNHTVPAGLKSALEWLSFKIHPLENKP
                     VMVVGASYYDQGTSRAQLHLRQILDAPGVNALVMPGNEFLLGKAKEAFDQEDRLIDPR
                     TVDFLGSTLASFVKFIKVVSTLAGPKKLPPEDLHATGTIATTIPDVDMSDDHWLEKAS
                     EAVGAVSGNTYVKLNRGLLTVDQINYFLDSMPMELTFADNNNQFLYYNLRQEAKEMLA
                     ARVPGQVGNPLANCHPEKSYKNVEWVIQQLRSGATDAVRVHVPKQVPEKYIVHNYQAM
                     HDENGEYAGINEYILDFKPIIEWYLQQTGQQLVGNEDAVSSASVHGGKTDAVTSASTH
                     DETAVDAVSSASVK"
     gene            complement(1305..1910)
                     /locus_tag="IV334_002634"
     CDS             complement(1305..1910)
                     /locus_tag="IV334_002634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005234653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HAP8944650.1"
                     /translation="MKFIGLVGTNSTRSTNRELLQFMKNYFVNEAEIEIVEIKELPLF
                     NKPENTELPNVVKEIAGKIEQADGVIISTPEYDHAVPASLINALNWLSYGIFPFVDKP
                     VMITGASYGTLGSSRAQAHLRQILDSPELKARIMPSSEFLLGHSLQAFDEQGRLIDEE
                     QVAKLRELFKDFLLFVTITSQLQHAHQANKREAESFSWETI"
     gene            complement(1959..2891)
                     /locus_tag="IV334_002635"
     CDS             complement(1959..2891)
                     /locus_tag="IV334_002635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HAP8944651.1"
                     /translation="MNQVIEETKIWQMMGTTITLQVGHEEPSRLLAELGEWLHVYEHR
                     FSAHDATSELMAINQAAGRQAVIVHPELFELIKLGKAHSCARNSYLNIAIGPVVQKWH
                     IGFEDALVPTKAEITELLLLTDPHQIQLDEKMKTVFLTKPGMMIDLGALAKGYIADKL
                     ITHCRERNAAFALVNLGGNIVVFGESPRRSDGAWRIGLRNPKGSREESLAVVKLNNQS
                     LVTSGIYERYLQNGSSVYHHIIDPKTGFPVKNDVASLTILSDSSVDGEIWTTRLFGAS
                     SLEVLKALEEQPGIEGAVVTKENQFLYTKGFEKI"
     gene            complement(3312..4172)
                     /locus_tag="IV334_002636"
     CDS             complement(3312..4172)
                     /locus_tag="IV334_002636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP8944652.1"
                     /translation="MNIEKFITARKAANLSQKEVSEGICTQATLSKFENNGQVPSLKI
                     LIKLCNRINLPLVELFSSMNVINTQLDEQLEKAEFSLITSEYEQLTQILAAIDPKVID
                     ESELIARYYYLKGFEMIFTNAPITDVLFHFDQLLMAETANTLFRLLAYTGIGMVYARE
                     KELEKAEVYFNRVFEKIYTYPIRTTEDIWRVLNIVFHCGVFFAQDGDLETSDALLNYA
                     IDICSDNHLTYYLARAAAQLAKNAQAKNKSKAEVLELIQDALAFAKINKNKKLIDELN
                     HMKKMILLIN"
BASE COUNT         1230 a          866 c          707 g         1507 t
ORIGIN      
        1 agcttaaatc atagataccg taagggactt tattctttat ttaaaacttt gcaacagaac
       61 ctttttattt tacagaagca ctcgaaactg catctactgc agtttcatcg tgtgtgctgg
      121 cgctggttac tgcatctgtt tttccaccat gaacagatgc acttgaaacc gcatcttcat
      181 ttccaacaag ttgttgaccc gtttgttgta aataccactc aatgattggt ttgaaatcaa
      241 gaatgtattc attgattcca gcatactctc cattttcatc atgcattgct tggtaattat
      301 gcacaatata tttttctggc acttgttttg gtacatgaac acgtaccgca tctgttgctc
      361 cgcttcgcag ttgttgaatt acccactcta catttttata cgatttttct gggtgacagt
      421 tggctaatgg attccctacc tgaccgggca cacgagcagc taacatctct ttcgcttctt
      481 ggcgcaaatt ataatataaa aattggttat tattatctgc aaaagttaat tccatcggca
      541 tcgaatctaa aaagtaattg atttgatcca ctgtcaataa cccacgattt aatttgacgt
      601 aagtatttcc agaaacggca ccgactgctt cagaagcttt ttccaaccaa tgatcatctg
      661 acatatcaac atcgggtatt gtcgtagcaa ttgttcctgt cgcatgcaaa tcttctggtg
      721 gaagcttttt aggccctgct aaggtagaca cgactttaat gaattttaca aaacttgcta
      781 atgtactgcc taaaaagtca acagttcgtg gatcaatcaa gcgatcttct tggtcaaatg
      841 cttcttttgc ttttcccaat aaaaattcat ttccgggcat cactaatgca ttcacgcctg
      901 gtgcgtccaa aatttggcgc aagtgtaatt gcgcgcgtga agttccttga tcgtagtaag
      961 aagcacccac aaccattaca ggtttatttt caagtggatg gattttaaat gatagccatt
     1021 caagtgcact tttcaatcct gctggaaccg tatgattgtg ttctggcgta gcaataatta
     1081 ccccatcagc cattaagatt ttgcgattta gctgttgaat agcaggacta tttgtttgat
     1141 catcatcttg attgaacaga ggtaaatctt tgatttcaat gatttctaaa ttaaatagtt
     1201 catgatattg tttttggata aatgttaata atttacggtt atatgaaagt tcagctgctg
     1261 atccgacgat acctattaat ttcatctttg tttgctcccc tcgattaaat cgtttcccat
     1321 gaaaagcttt cggcttcacg tttatttgct tggtgcgcat gttgcaattg actagtgatt
     1381 gtgacaaaga gtaagaaatc tttgaatagt tcacgtaatt ttgcgacttg ttcttcatca
     1441 atgagtcgtc cttgctcatc aaaggcttgg agtgaatgac ccaataaaaa ttctgagctt
     1501 ggcataattc gtgcctttaa ctctggtgaa tccaatattt ggcgcaaatg tgcttgtgcg
     1561 cgtgaagaac ctaacgtgcc ataagacgct ccggtaatca tcactggttt atccacaaac
     1621 ggaaaaatac catatgaaag ccaatttaat gcgttaatca atgaagcagg aaccgcatgg
     1681 tcatactctg gtgtgctgat gattacgcca tctgcttgtt caatttttcc agcaatttct
     1741 ttaacaacat ttggtagttc tgtgttttca ggtttgttaa atagtggcag ctccttgatt
     1801 tcgacaattt cgatttctgc ctcatttaca aaataatttt tcataaactg taataattca
     1861 cggtttgttg accgcgttga atttgttcct actaatccaa taaatttcat cttcattctc
     1921 tttcctttct aacatcgatt tctgactgtt cgcacgtact aaattttttc aaatcctttt
     1981 gtatacaaga attgattctc ttttgttaca actgctccct cgatacccgg ctgttcttca
     2041 agcgctttca acacttctaa agaagatgct ccaaataaac gtgtagtcca gatttctcca
     2101 tctacagaag aatcagacaa aatcgtcagg cttgctacgt catttttaac tggaaagcca
     2161 gttttcggat caatgatgtg atgatacact gacgaaccat tttgtagata acgttcgtag
     2221 attccagagg taactaaaga ttggttgttt agtttaacca ccgcaagcga ctcttcacgt
     2281 gaacctttag gatttcgcaa accaatacgc caagcaccat ccgaacgtct tggtgattca
     2341 ccaaaaacaa cgatatttcc tcctaagtta actaaagcaa acgctgcatt tcgctctcgg
     2401 caatgtgtaa ttaatttatc tgcaatgtaa ccttttgcta acgcccccaa gtcaatcatc
     2461 attcctggtt tggttaaaaa aacggttttc attttttcat ctaattgaat ttggtgtgga
     2521 tccgttagca gaagtaactc agttatctct gcttttgttg gaaccaatgc atcttcaaat
     2581 ccaatgtgcc atttttgtac aaccggacca atagcaatat tcagataact atttcttgca
     2641 caactatgtg cttttcctaa tttgatgagc tcaaaaagtt caggatgtac aatcacagct
     2701 tgtcgtccag cagcttgatt gatggccatt aattcagaag ttgcatcatg tgcgctgaag
     2761 cgatgttcat aaacgtgcaa ccattcgcct aactctgcta gtaatcgact aggctcttca
     2821 tgcccaactt gtaatgtaat tgttgtcccc atcatttgcc agattttcgt ttcttcgatc
     2881 acctgattca ttttgtacct tctttacttg aactacggat tacatgagta attagttttt
     2941 atttttgtta ctgttttcac aaccagatga tacgcctgct agatttattt gtcaaccgat
     3001 tttatatatt tttttatata ataaggtaat tttaatataa aattttatat tgtaaaaaaa
     3061 caaataaggg aaacaaacaa cacatttttg tgtgttgctt atttccctct aactatcgtt
     3121 ttctatttga tggttaacct acttaaaata aatactgcaa cgacaaacgg agatcaagca
     3181 tgctttgtaa cgtattttac gataattacg actttatttt attactctat cttgctgcca
     3241 tacccgtcta tccctaatca tctttggtga attttttcaa agttagcata acaaaaataa
     3301 gtattttttc cttagttaat caatagtatc atttttttca tatgatttaa ttcatcaatt
     3361 aattttttgt ttttattaat tttagcaaaa gctagtgcat cttgaatcaa ttcgagcact
     3421 tctgccttcg acttattttt agcctgtgcg ttcttcgcta attgggcagc agctcgtgct
     3481 aagtaataag tcaaatggtt atccgaacaa atatcgattg cgtaatttag tagtgcatca
     3541 cttgtttcca aatcaccgtc ttgcgcaaaa aaaacaccac aatgaaagac gatatttaac
     3601 acccgccaga tatcttctgt tgtacgaatc ggatacgtat aaattttttc aaacactcga
     3661 ttgaaataaa cttctgcttt ttccaactct ttttctctag catagaccat tccaatacct
     3721 gtatatgcca ataaacgaaa caacgtgttg gctgtttctg ccatcaacaa ctgatcaaaa
     3781 tggaacaaca catccgtaat tggtgcattc gtaaaaatca tttcaaatcc ttttagatag
     3841 tagtaacgtg caatcagctc agactcgtca attaccttgg gatcaatggc tgctaaaatc
     3901 tgtgtcaatt gttcatattc actcgtaatc agtgaaaact ctgctttttc tagctgttca
     3961 tcaagttgtg tattgatcac gttcatcgaa gaaaaaagtt caactaacgg aagattgatt
     4021 cgattacata atttgattaa aatttttaac gaagggactt gaccattgtt ctcaaattta
     4081 cttaatgttg cttgtgtaca aattccttca gacacttctt tttgagataa gttggcagcc
     4141 tttcttgccg taataaattt ttcaatgttc attattttct ctccaatcag acctcacacg
     4201 aaaatcattt ctgtctcgat tttagcatgt tttctttggg aatgctttag gttctgttgc
     4261 aaagttttaa ataaagaata aagtccctta cggtatctat gatttaagct
//
LOCUS       DACDKM010000153         4272 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20834_2016
            SAMN12640370-rid9891663.denovo.154, whole genome shotgun sequence.
ACCESSION   DACDKM010000153 DACDKM010000000
VERSION     DACDKM010000153.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640370
            Sequence Read Archive: SRR10040891
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4272)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4272)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742835.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742835.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:04:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,012
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 29 of 75
            Pseudo Genes (incomplete)         :: 33 of 75
            Pseudo Genes (internal stop)      :: 19 of 75
            Pseudo Genes (multiple problems)  :: 5 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4272
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640370-rid9891663.denovo.154"
                     /isolate="E20834_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            1..492
                     /locus_tag="IV334_002637"
                     /pseudo
     CDS             1..492
                     /locus_tag="IV334_002637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338872.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            712..1008
                     /locus_tag="IV334_002638"
                     /pseudo
     CDS             712..1008
                     /locus_tag="IV334_002638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176405.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2479 domain-containing protein"
     gene            1171..1617
                     /locus_tag="IV334_002639"
     CDS             1171..1617
                     /locus_tag="IV334_002639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8944653.1"
                     /translation="MKNIWKYGRTGGEYAGKVLDDMLVSVPYTDQPPLEGIRADGEPL
                     TIADQMFDPKLNQWIILANALDHNDLNNLKAMYESLENENGDLKQINAKLMLSDVAIK
                     QENTALKEKADSLAQINSKMMLASLQNSKDISEIKEQLNPASKGGE"
     gene            1619..1756
                     /locus_tag="IV334_002640"
     CDS             1619..1756
                     /locus_tag="IV334_002640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XkdX family protein"
                     /protein_id="HAP8944654.1"
                     /translation="MFSFSDVKMMYDWGCFTNEQVMVFVPLCITEEKADKIISKEESA
                     S"
     gene            1794..2087
                     /locus_tag="IV334_002641"
     CDS             1794..2087
                     /locus_tag="IV334_002641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8944655.1"
                     /translation="MINLGEWGAIAGSITAIVSLILLVIKPITASFSKITETLSKVSH
                     NLDLLTKDLESSKSDRLMIHEELKKHDERLDTHAEKLVEHTQQIKTLFRERRK"
     gene            2084..2308
                     /locus_tag="IV334_002642"
     CDS             2084..2308
                     /locus_tag="IV334_002642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holin"
                     /protein_id="HAP8944656.1"
                     /translation="MNNKTFEVLKWFALVIIPALATFVGLVGKALNWQYTDICVVIIT
                     GFGAFLGSVLGVSNRTYKMFSAESEEGGNK"
     gene            2305..3330
                     /locus_tag="IV334_002643"
     CDS             2305..3330
                     /locus_tag="IV334_002643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HAP8944657.1"
                     /translation="MKKKITITAMSLLTALFLLPINTFAYTINDEYNLAPNQGDSRLA
                     IPNKIILHETGIDAPARNVAANMKNNYNGSNSYTTDVIGDGGIVYRVGEQGYVSWGAG
                     NANPYAPVQIELQRTYDKALFEKNYRAYIEYTRDSAKKYGIPLTLDQGTSLFTKGIIS
                     HLWVTNYVWGNHTDPYGYLSQMGVSKEKLAYDLAHGFTDENPTTSENKPVIDPTRAGA
                     ANPTLTDGTNHAHIDQFGEIENANLHVAGWHIANYKYEYIFIMDYNTGKELARVRADG
                     IYRPDVNQAYNTSGNVGYHVSFNMRNFPSKKVYVMMRATNDPEGNTKGGAQDFHDKRW
                     YLNIPKR"
     gene            3520..3593
                     /locus_tag="IV334_002644"
     tRNA            3520..3593
                     /locus_tag="IV334_002644"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3554..3556,aa:Ile,seq:cat)
BASE COUNT         1484 a          729 c          858 g         1201 t
ORIGIN      
        1 ctacttgatg caccgtatta tttgagtaag gtggctttgg gtgagaatct aattaaacca
       61 gaatcacaac aaccagttac taatagtaac tatcttatta acacctataa cattaaacca
      121 atggtaaaag gtaagaagta taccatcaca cttgaaggaa ctaagccagc aacacaggtt
      181 tttagaccat ttttcacacg agcaacagga gatgcatggg aggttggtga cttacaacca
      241 gtagaagggt taactgatgt ttggtctgga acatttacag cagcagctga cccacaccct
      301 accagcccac aagttcagat ttgtcaggta ccaagcacaa gtttaggaaa ctgtacaatt
      361 aaatggttaa aactagagga aggcgacaca cgaaccccga atattgagca atataaatac
      421 cgaggaatcg gcatgcgaga ctcaaacaat ccaaaagatt acgtctggga tctagcacca
      481 gaatatgtcg aagacaatct ggccacagat attaaaattt ctgaaattac tggtaaagca
      541 aacaattata ccgatgggaa agtatcggag attaattcgc agttgactgc ttcaattaat
      601 gaagtagaca ccacagctaa ggatgctcaa acaaaagcga atgctaatgc gactgctata
      661 gatgaattag acaataagat cgatgaacgc attaatgata cagctactac cacattaaca
      721 gttacaaacg ggaataccgg atcagcaaag ctttatcgtg aaggaaaaac agtttctata
      781 tattttgtgg ctttaaacgg aagaagcagt ggtggaaatg attcaacgat actaacaatt
      841 ccagaaggct atcggccacc aattagtttt gagcaactgg ttggctcgat agaccgttct
      901 actttgaaca gtgctcagtt atctattggt gcagatggag ccattaaatg gcgaagaaac
      961 tcaagttatg gatcggatta tacctttgca attacttaca cgatttagaa aagcgtgaat
     1021 cgatatgaag gcagcatata gaccaattga accttacgga ttcgagcaaa tcattgtgaa
     1081 tgatgaagaa catttaccgg aagaatgcac agaagtcgaa ccaccgattc caaattggaa
     1141 accgaaattc aattactggg agggaaataa atgaaaaaca tttggaaata tggacgtact
     1201 ggcggagagt acgcaggaaa agtattggac gacatgcttg tatccgttcc ttacacagat
     1261 cagccaccgc ttgaaggaat tcgtgctgat ggcgaaccgt taacaatcgc tgatcaaatg
     1321 tttgatccta aattgaatca atggattatt ttagcgaacg cactagatca caacgattta
     1381 aacaatctca aagcaatgta tgagtcgtta gaaaatgaga acggcgattt aaaacagatc
     1441 aatgccaaac tcatgctaag cgatgtagca attaaacagg aaaatactgc attgaaagaa
     1501 aaagcggata gtttagcaca aatcaattca aaaatgatgc ttgcttcgtt acaaaatagc
     1561 aaagacattt cagaaattaa agagcaacta aatccagctt caaagggagg tgagtagtat
     1621 gtttagtttt agcgatgtga aaatgatgta tgattggggc tgtttcacta acgaacaagt
     1681 aatggttttc gttccgttgt gcattactga agaaaaagca gataaaatca ttagcaaaga
     1741 agagagcgca tcttaattga tgtgcttttt attttgattc aaggagttgt cacatgatta
     1801 atttagggga atggggagcg atagcaggat caataaccgc tatcgtttct ttgattttat
     1861 tagtaataaa accaattact gcatctttct cgaagattac tgagactctt tcaaaagtaa
     1921 gtcacaattt agatttgctg actaaagatt tagaatcgag caaatcagat cgattgatga
     1981 ttcatgaaga actaaagaaa cacgatgaaa gattagatac acatgcagaa aaattggtag
     2041 aacacacgca acaaattaaa actttattta gggagagaag aaaatgaata ataaaacgtt
     2101 cgaagtacta aaatggttcg cactggtaat tattcccgca ctagctactt tcgtggggtt
     2161 agttggtaaa gcgctcaatt ggcagtacac agatatctgt gttgtcatca ttactggttt
     2221 tggcgcgttt ttagggagtg tgttgggtgt atcaaatcga acctacaaaa tgttctcggc
     2281 tgaaagcgaa gaaggaggaa acaaatgaaa aagaaaatta ctattactgc gatgagcctg
     2341 ttaacggctc tttttttatt acccattaat acgtttgctt atactattaa tgacgagtat
     2401 aatttagcgc cgaatcaagg agactccaga ttagcaattc ctaacaaaat tattttgcac
     2461 gaaactggaa tagatgcacc agcaagaaac gtagccgcca acatgaaaaa taattataac
     2521 ggaagcaatt cttatactac agatgttatt ggtgacggtg ggattgttta ccgtgtgggt
     2581 gagcaaggat atgtttcgtg gggagctggt aacgctaatc cttatgcgcc tgtacagatt
     2641 gaattacagc gcacatatga taaagcattg tttgaaaaaa actatcgagc ttatattgaa
     2701 tatacaagag atagtgcaaa aaaatatgga attccattga ctcttgatca aggaacttct
     2761 ttatttacaa aaggaatcat ttctcatttg tgggtgacaa attatgtttg ggggaaccac
     2821 acagatccat atggttactt atcgcaaatg ggagttagca aagaaaagct tgcttatgat
     2881 ttagctcatg gatttaccga tgaaaatcca acaacttctg aaaacaagcc tgtcattgat
     2941 ccaacacgag ctggtgcagc taatcctacg ctgacagatg gaacaaatca cgcccacatt
     3001 gatcagtttg gggaaatcga aaacgcaaac ttgcatgtgg ctggatggca cattgctaac
     3061 tataaatacg agtatatttt cattatggac tacaatactg ggaaagaatt agctcgagta
     3121 agagctgatg gaatttatag accagatgta aaccaagctt ataatacttc tggaaatgtt
     3181 ggttatcatg tatctttcaa tatgcgtaat tttcctagta agaaagtcta tgtaatgatg
     3241 cgggcaacga atgatccaga gggaaacact aaaggcggtg cgcaagattt ccatgacaaa
     3301 cgttggtatt taaatattcc taaacgataa aaatagctcc tcgttgagga gcagtacata
     3361 actatattga caactataaa aattattcga taaaatagtg atgttatcgc atatcttcac
     3421 tatcacccat aaatagtcac actccaagct atgcgataac aggtttgttg ccacacattc
     3481 tactggttga ttgtttatgg ctttatgtgg caacaaccag tacccttagc tcagttggtc
     3541 agagcagacg gctcataacc gtccggtcgt aggttcgagt cctacagggt acattaacgt
     3601 agccatttga atcgttctgt gttagaattt ttttgaagag tattatacaa gctaaagctt
     3661 ttcttcattg ccactcaaat gagtggcttt tttatgtatc cttttatgga ttaatgaaag
     3721 gatgtttcac atagttatac ttctgtatat ttgaaaagtt ttactttgat ttttaaatag
     3781 aaagacattt gggttaaatt gtgagataat aataaagaag agtttaaagc gcaccccaaa
     3841 ccacttcccc ataagtgtgt tacgctttaa actcttttat atttgaagcc attaaaaagc
     3901 ataccatatt tttgaaaaaa agtgagaaaa aaggcttaca attggagtgg tagttaatta
     3961 gtgacttatt tttgatttta tagcactgat actataaaat atagatatca tcatattaca
     4021 caatcttaat actaacttaa aaaatatctc ctttcacaag tatggtgata aaattcgttc
     4081 cgggctacct ttttaggtag cctactttaa tctttatacc tttctggatc aacgaaagta
     4141 tactttatat agtcataacg ccgatgatcg ctacgtgcgt ccggcacgtc agtcatgaat
     4201 cggctacact agactagaca gaaaaaataa ggtgtgtaga ataataaaaa acacactgga
     4261 ggatttttca tg
//
LOCUS       DACDKM010000154         4223 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20834_2016
            SAMN12640370-rid9891663.denovo.155, whole genome shotgun sequence.
ACCESSION   DACDKM010000154 DACDKM010000000
VERSION     DACDKM010000154.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640370
            Sequence Read Archive: SRR10040891
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4223)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4223)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742835.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742835.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:04:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,012
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 29 of 75
            Pseudo Genes (incomplete)         :: 33 of 75
            Pseudo Genes (internal stop)      :: 19 of 75
            Pseudo Genes (multiple problems)  :: 5 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4223
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640370-rid9891663.denovo.155"
                     /isolate="E20834_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            14..1087
                     /locus_tag="IV334_002645"
     CDS             14..1087
                     /locus_tag="IV334_002645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8944658.1"
                     /translation="MLANTYYQIIVAIQMKNTIFSDEYVEIIISDHSKNTKEIAKKIE
                     EKKVFDGCHYLESKSFDDNIGYFQKIKDCLQISCGKSNRYAYYLKNCKSEYFDEFICF
                     SMGEVWLEGIYACLYRNNKNVQVSILEEGILSYGVDIIPTARTKAIAVLNYLKKRTPL
                     YNKSYNFYCFFPNIYTGRENTIQIPLVTAGSRTALQLSDIFNPMIDIYKEKKYIFFTS
                     VYDFEGGEPVGEYELVCKIADLVGKDNLIVKTHPRDVRTIYKDNGFIEDKNSSIPWEA
                     IQLSGDFNDKIFMTINSGSVISGSTMSAKPVRTYYMYKLCNIEGNPSCKKNAYDIERL
                     LKQKTMKNILKNVKIAESLDDIL"
     gene            1084..2505
                     /locus_tag="IV334_002646"
     CDS             1084..2505
                     /locus_tag="IV334_002646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HAP8944659.1"
                     /translation="MSKTKYLIKNMGILTISNFSSKILVFLLVPLYTSVLSTTEYGTY
                     DLTISTATLLFPILTLNIVDAVMRFLMDKDKNKKNIASIAIKVIFLSVIVFGSVMYLL
                     NITKLWQDVYGLEKYIFLYYISYVWNQFLIQFAKGLEKVKAMGIAGIISTIVTIIANI
                     FFLLILKWGLIGFFIANILSQIISATYLSVLVRIWEYIDFEAKDPQLKREMLNYSIPL
                     IATVVGWWINSTADRYVVSFIVGVSANGLLSVAYKIPQIINTLQGIFIQAWQISAIKE
                     YGDNETAKFYGNTFVMINLLMCITCSSLIILTRPFSHILYAKDFYEAWKYVPFLLIAN
                     VLNCASGLLGPILSAKKDSKSMMWSAIIGALVNVIMSVVLVYKIGVQGATIATVICSY
                     VIYVVRKRAVGKDIFIKHYHIVVESWGLLFVQAIIEIYFKNYVIEIIIMIILIIINIS
                     YIKQLCYRGRNIISMIKSREHKR"
     gene            2598..3257
                     /locus_tag="IV334_002647"
     CDS             2598..3257
                     /locus_tag="IV334_002647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylneuraminate cytidylyltransferase family
                     protein"
                     /protein_id="HAP8944660.1"
                     /translation="MKIVAIVPMKLNNKRLPQKNTKAFTNGKPLCYYILSTLLKVKQI
                     DKVYVYCSNPDIKEFIPEGVTFLKRSSSLDQDTTSITQVLAGFTKEVEADVYVMTHTT
                     APFISEKSIEKGLDAVLTGEYDSAFAAKKLQDFLWKDGEPFNYELDNIPRTQDLPPLY
                     EETSGFYIYNRNVMTELHRRIGKKPCIVEVGEIESVDIDEAEDFMIADAIYNHLILQG
                     S"
     gene            3276..>4223
                     /locus_tag="IV334_002648"
     CDS             3276..>4223
                     /locus_tag="IV334_002648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxy-3-methylglutaryl-CoA lyase"
                     /protein_id="HAP8944661.1"
                     /translation="MNNVQILDCTLRDGGYCNNWEFGFDNTKKIISGLIESGIEIIEC
                     GFLTNKTVYNKNVTKYTTLEELAQIIPEKREGKLFVAMMNYGEYNIEDLPDYDGSSID
                     GIRIAFHKKNCNEALKLCREIKKKGYLVFVQAMVSLNYTDEEFLAVIKNVNQFKPYAF
                     YIVDSFGMMKGKDLTRLFYMIEHNLEKDIQIGFHSHNNMQLAYSNAQKLITVQTNRSL
                     IIDSSVYGMGRGAGNLNTELFVEYLNDNAGKKYQLKPLLYIIDEILNSFYQKSYWGYS
                     LPNYISAVHNAHPNYASYLSYKNTLTIEAMNEIFEMMDEE"
BASE COUNT         1577 a          492 c          758 g         1396 t
ORIGIN      
        1 gaaaaaaatt ttagtgttag caaatacata ttatcaaata atagtagcaa tacaaatgaa
       61 aaatacaatt ttttccgatg agtatgttga aattattatt tctgatcatt ctaaaaacac
      121 taaagaaatt gcgaaaaaaa tagaagaaaa aaaagtgttt gatggctgtc attatttaga
      181 aagcaaatct tttgatgata atattgggta ttttcaaaag attaaggact gtcttcagat
      241 ttcttgtggt aaatcaaatc gatatgccta ttatttgaaa aactgtaaaa gtgaatattt
      301 tgatgaattt atttgttttt caatgggaga ggtttggttg gaaggaatat atgcttgctt
      361 atatcgaaat aataaaaatg tacaggtatc aattttagaa gaaggtattt taagctatgg
      421 agttgacatt attccaacag caagaacaaa agctatcgca gtactcaatt acttaaaaaa
      481 aaggactcca ctatataaca aaagctataa tttttattgt ttttttccta atatatatac
      541 aggaagagag aatacaatac aaattccatt agttacagct gggtcaagaa ccgcattgca
      601 actttcggat atttttaacc ctatgataga tatttataaa gaaaaaaaat atatattttt
      661 tacgtctgtt tatgattttg agggcggaga acctgtcgga gaatatgaat tagtatgcaa
      721 aatagcagac ttagtaggaa aagataatct aattgttaag acgcatccta gagatgtaag
      781 aacaatttat aaagataatg gatttatcga ggataaaaat tcatcaatac cttgggaggc
      841 gattcaattg tcaggcgatt ttaacgataa aatatttatg acaattaatt caggaagtgt
      901 tatttcaggt agtacaatgt ctgcaaaacc tgtacgcacg tattatatgt ataaattgtg
      961 taacatagaa ggaaatccgt cttgcaaaaa gaatgcgtat gatattgagc gattgcttaa
     1021 gcaaaaaacg atgaaaaaca ttttaaagaa tgtgaaaata gcagaaagct tagatgatat
     1081 actatgagca aaactaaata tttaattaaa aatatgggaa tattaacaat aagtaatttt
     1141 tcttcaaaaa ttttagtttt tttgttagtc cctttatata caagtgtatt atccacaaca
     1201 gaatatggca catatgattt gacaatttca acagccacat tattgtttcc aattttaacc
     1261 ttaaacattg tagatgctgt gatgcgcttt ttaatggata aagataaaaa taaaaaaaat
     1321 atagcatcta tagcgattaa agttatattt ttaagcgtga tagtctttgg atccgtaatg
     1381 tatttgttaa atattacaaa attatggcaa gatgtttatg gattagaaaa atatattttt
     1441 ttgtattata tatcctatgt atggaatcaa tttctaatac aatttgctaa agggttagaa
     1501 aaagttaaag ctatgggcat agcaggtatt atcagcacaa ttgtaacaat aattgccaat
     1561 attttcttct tgcttattct gaaatgggga ttaattggtt ttttcattgc aaatatttta
     1621 tcacagatta tttcagctac ttatctgtca gtattagtga gaatatggga atatatcgat
     1681 tttgaggcta aagatcctca gctaaagaga gaaatgctaa attactcgat tccattaatt
     1741 gcaactgttg ttggatggtg gattaatagt acagctgata ggtatgtggt ttcctttata
     1801 gtaggtgtct cggcgaatgg cttattgtct gtggcatata aaatacctca aataattaat
     1861 actttgcagg gaatatttat acaggcctgg caaatttctg caattaaaga atatggagat
     1921 aatgagactg caaaatttta tggaaataca tttgttatga ttaatttgtt aatgtgcata
     1981 acctgttcaa gtcttatcat attaactaga ccgttttcac atattcttta tgcgaaagac
     2041 ttttatgaag cgtggaaata tgttccattt ttattaatag ctaatgtttt aaattgtgca
     2101 tcaggtctgt taggacctat cttatcagca aaaaaagatt ctaaatccat gatgtggtct
     2161 gcaatcatag gggcattagt taatgtgatt atgagtgtag tccttgttta taagataggg
     2221 gttcaaggtg ccacaatagc cactgttata tgttcatatg taatatatgt tgttcgtaaa
     2281 cgagctgtgg gaaaagatat ttttataaag cattatcata ttgttgtgga atcttgggga
     2341 ttattgtttg ttcaggcaat catagaaata tattttaaaa attatgtaat agaaattata
     2401 attatgatta ttttaattat tataaatatc agttatatta agcagttgtg ttatagagga
     2461 agaaacatta tttcaatgat taagagtcga gagcacaaac ggtagacgat gttattttta
     2521 taaaaagata taatttatta atggtcgata attatgaaag gaaaaatatt cttaaaaaaa
     2581 agagtatagg atgtcgtatg aaaatagtcg ctatagtacc aatgaagtta aataacaaaa
     2641 gattgccaca aaaaaacacg aaagctttta ccaatggaaa accattgtgt tattatatac
     2701 tttcaacact tttaaaagta aaacagattg ataaagtgta tgtctattgt agtaacccag
     2761 atattaaaga atttatacca gaaggtgtaa catttttaaa gagatctagt tccttagatc
     2821 aggatacaac gagtattaca caggtattag ccggtttcac aaaagaagta gaggctgatg
     2881 tatatgtaat gacacacaca acagcaccat ttattagcga aaaatctatt gaaaaaggac
     2941 tagatgccgt gctgacagga gaatatgatt ctgcatttgc tgcaaaaaaa ttgcaagatt
     3001 tcttatggaa agacggtgaa ccatttaatt atgaattgga caatattcct agaacacagg
     3061 atttgccgcc tttatacgaa gaaactagtg gattttatat ttataatcgc aatgtgatga
     3121 ccgagttgca tagaagaatt gggaaaaaac cttgcatagt tgaagtaggt gagattgagt
     3181 cagttgatat tgatgaagcc gaggatttta tgattgctga tgcgatttat aatcatctta
     3241 ttttacaagg tagttaagac agggggaagg ttaggatgaa caatgtacag attttagatt
     3301 gcacacttag agatgggggc tattgtaaca attgggaatt tggatttgat aatacaaaaa
     3361 aaattatttc tggacttata gaatcaggaa ttgagattat agaatgtggg tttctcacaa
     3421 ataaaacagt atacaataaa aatgttacaa aatatacgac cttagaagaa ttagcacaga
     3481 taattcctga aaaaagagaa ggaaaattat ttgtagcaat gatgaactat ggcgaatata
     3541 atatagagga tcttccagat tatgatggtt catctattga tggaattagg attgcgtttc
     3601 ataaaaaaaa ttgcaatgaa gcgttgaaat tatgtcgtga gataaagaaa aaaggctact
     3661 tagtttttgt tcaagctatg gtatctctca attatacaga tgaagagttt ttagcagtaa
     3721 taaaaaatgt gaatcagttt aaaccatacg ctttttatat tgttgatagt tttggaatga
     3781 tgaaaggaaa agatttaact cgtctttttt acatgattga gcataatcta gaaaaagata
     3841 tacagatagg ttttcactca cataataaca tgcagcttgc atattctaat gcacaaaaac
     3901 ttataaccgt acaaacaaat cgaagcctga ttattgattc ttcggtgtac ggaatgggaa
     3961 gaggagcagg taatcttaac acagaactat ttgtagaata tttgaatgat aatgcaggaa
     4021 aaaaatatca attaaaacca ttattatata taattgatga aatactaaat agtttctatc
     4081 agaagagtta ttggggatat tcattaccaa actatatttc agcagttcat aatgctcatc
     4141 ctaattatgc aagctatctg tcatataaga atactttaac aattgaagca atgaatgaaa
     4201 tattcgaaat gatggatgaa gaa
//
LOCUS       DACDKM010000155         4218 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20834_2016
            SAMN12640370-rid9891663.denovo.156, whole genome shotgun sequence.
ACCESSION   DACDKM010000155 DACDKM010000000
VERSION     DACDKM010000155.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640370
            Sequence Read Archive: SRR10040891
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4218)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4218)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742835.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742835.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:04:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,012
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 29 of 75
            Pseudo Genes (incomplete)         :: 33 of 75
            Pseudo Genes (internal stop)      :: 19 of 75
            Pseudo Genes (multiple problems)  :: 5 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4218
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640370-rid9891663.denovo.156"
                     /isolate="E20834_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            138..1475
                     /locus_tag="IV334_002649"
     CDS             138..1475
                     /locus_tag="IV334_002649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate transporter"
                     /protein_id="HAP8944662.1"
                     /translation="MSDIIIGILLVISFFFMVWYCVKGYNLMVGFAIMATVWMGLALV
                     GNTFSPNPAMEGQGVIDVLTHIYTTGPAEYAKSILVNVFFGAFFGRVLVDSGIAATLI
                     RKVVELGGDKPRITMSLLCIVTAVIFMSMTGIGPVISIAVIVLPILMSLGISVPVALF
                     SFMGSIMAGIFANIVNFKQYQTIYAGFNPAAESYTYNDYFQIGMIGMIVSLVVVLTVA
                     NISMNKKKRYAMAANVPAEGGDAPMISWLAVLLPVLGVVLLDLPIILGFILAGIWALL
                     FTGKLRGGYKEICRQFAKLFTDGAVDVAPMVGFLMTLAMFNNSAAYASPYFSAIFGDW
                     IPQSPLVLAIVFAILTPLGFFRGPMNLVGSGSAILAVVLAVNPTMSPAFLFPLFAITT
                     IAPQHLDITQSWVAWGLGYTKVTSREYMKKSIPTGWIIGAILCLITFLLYGNA"
     gene            1523..3652
                     /locus_tag="IV334_002650"
     CDS             1523..3652
                     /locus_tag="IV334_002650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase family protein"
                     /protein_id="HAP8944663.1"
                     /translation="MKRFVRMGIDVGGTHTKAVAIDNATHEIIGKSSVKTTHDDVRGV
                     AAGVVQSFQNCLLENNISPEDVVFVAHSTTQATNALIEGDVAKVGVIGMAKGGLEGFL
                     AKRQTRLNDIDLGNKKKIEIVNAFLPVKHLNVDRVSETISSLERERAEVLVSSMAFGV
                     DNGEPERVVYEAASVKSIPTTMASDITKLYGLTRRTRTAAINASILPKMLDTATSTED
                     SVREAGVNVSLMIMRGDGGVMEINEMKKRPVLTMLSGPAASVMGSLMYLRASNGVYFE
                     VGGTTTNIGVIKNGRPAIDYSIVGGHPTYISSLDVRVLGVAGGSMVRANQSGIIDVGP
                     RSAHIAGLDYAVFTETEKIKGPKVEFFSPKEGDPADYVKVVMEDGEEVTITNTCAANV
                     LGLVQEEHFSYGNVPSARKAIQALADYCHTTVEDIAEQIMEKSYAKIEPVILELADKY
                     HLEKDQISLVGVGGGAASLITYFSNKMGVKYSIPENAEVISSIGVALAMVRDVVERII
                     PSPSKEDIRSLKNEAMNKAIESGATPESIEVHVEIDPQTSKVTAIATGSTEVKATDLT
                     KEITTEEALELAAEDMRLNKNEVCLLENTPFFYVCGEQNRSKNAGSLRIIDQKGFIKV
                     QRGHASCMKTTAANYMTAVEQLWEDMAVYQTELIARPEFYLCLGARVSDFTATDLEQL
                     QLLMDLEVSTMEPEEEVIVVAGNIKQT"
     gene            3627..>4218
                     /locus_tag="IV334_002651"
     CDS             3627..>4218
                     /locus_tag="IV334_002651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8944664.1"
                     /translation="MPEISNKHKLEELLNELQQIPEEIWGFYQFRRDLFWKKIPLSKQ
                     KILIQQSIDCGIETACSIKKKYPFADVGEICEQMAVPIVSCESEQINERITFATYAED
                     EGIRLMTEPLEKLKCSGLASISKETAQALIIGHELFHHIEASVKGIYTQSEKIVLWRL
                     PFYTHQSNIRALSEIAAMSFSKEMNQSDFSPYVLEAV"
BASE COUNT         1298 a          753 c          957 g         1210 t
ORIGIN      
        1 cataattaag gttttttaat tgaaaactga acatttatgg tcacccttta atgaaagcgc
       61 taactacata agttagaatg agtttgactg atatattagt ttgcttatca gaaaaacaaa
      121 atggacggag gaataacatg agtgatatta ttattggtat tttgttagtt atctcattct
      181 ttttcatggt ctggtactgt gtaaaaggct acaacttgat ggtcggcttt gccatcatgg
      241 ctactgtatg gatgggatta gcgctagtcg gaaacacgtt ttcaccaaac ccggcgatgg
      301 aagggcaagg agtaattgat gtgttgacgc atatctatac gacggggcca gctgagtacg
      361 ccaaatcgat cttggtaaat gtcttctttg gtgccttttt cgggcgtgtt ctagtagata
      421 gcggaatcgc agctaccctg attcgaaagg tagttgaact tggaggggac aagccaagaa
      481 tcacgatgag cttactatgt attgtaaccg ctgttatatt catgtcgatg acaggaatcg
      541 gaccagtgat ttctattgca gttattgtgt tgccaatctt gatgtcatta ggtatttcag
      601 taccggttgc actgttctca tttatgggct cgattatggc tggaatcttt gctaatatcg
      661 tcaatttcaa acaatatcaa acgatttatg ctggtttcaa tccagcggct gaaagctata
      721 cttataatga ctatttccaa attggtatga ttgggatgat tgtaagctta gttgtggtac
      781 taacggtcgc taatatatca atgaacaaaa agaaacgcta tgcaatggca gcaaacgtac
      841 cagctgaagg tggcgacgca ccaatgatct catggttagc cgtattatta ccagttttag
      901 gggtcgttct tttagatctt ccaatcatct taggttttat tctagcaggt atctgggcat
      961 tgctgtttac tggaaaattg cgcggaggtt ataaagaaat ctgtcgtcag tttgcgaaat
     1021 tatttacaga tggcgcagta gatgttgcgc caatggttgg tttcttgatg accttagcga
     1081 tgttcaataa ttcagctgcg tatgcttctc cttatttttc agctattttt ggagattgga
     1141 taccgcaatc accattagtc ttggcaatcg tatttgctat attgacacca ctaggatttt
     1201 tccgcggtcc aatgaatcta gtcggttcag gctcagctat tttagctgtc gtattagctg
     1261 ttaatccaac gatgtcgcca gcctttttgt tcccgttatt tgcaatcaca acgattgctc
     1321 ctcagcattt agatattaca caatcttggg ttgcttgggg attaggttat acaaaagtaa
     1381 cttctcgaga atatatgaag aaatccatcc caactggttg gatcatcgga gcgatcttat
     1441 gcctgatcac gttcttattg tacgggaacg cctagtaaac aaaacgtcga taaaaacgat
     1501 tagatgaatg gaggaaaaga aaatgaaacg ttttgtgcga atgggaattg atgttggcgg
     1561 tacacatacg aaagcagtcg caatcgataa tgcgacgcac gaaatcatcg gaaaatcttc
     1621 agtgaaaaca acccacgatg atgtccgtgg agtagccgca ggtgtcgttc agtcttttca
     1681 aaattgtttg ctggaaaata atatcagtcc agaagatgtt gtgtttgttg cacattctac
     1741 cacacaagcg actaatgcac tgattgaagg agacgtagca aaagtcggcg taatcgggat
     1801 ggcaaaaggt ggattagaag gttttttagc aaaacgacaa actagattga acgatatcga
     1861 tttagggaat aagaaaaaaa tagagattgt caatgcattc ttacctgtca aacacttaaa
     1921 tgtagaccgt gtttcagaaa cgatttcttc tttagaaaga gaaagagcgg aggtcttggt
     1981 ttcttctatg gcatttggag tagataatgg ggaaccagaa cgagttgttt atgaagcagc
     2041 atcggtaaaa tcgatcccta caacgatggc ttctgacatc acaaaattat atggattgac
     2101 aagaagaact cggacagcag caatcaatgc atctatttta ccaaaaatgc tagatacggc
     2161 aacctctaca gaagattccg tacgagaagc aggggtcaat gtttctttga tgattatgcg
     2221 cggagacggc ggagtaatgg aaatcaatga aatgaaaaaa cgacctgttt taaccatgct
     2281 ttccggtcca gcagcatcag tcatgggctc tttgatgtac ttgagagcgt ctaatggtgt
     2341 ttattttgaa gtaggcggaa caacaaccaa tatcggggtc attaaaaatg gacgtccagc
     2401 aatcgattat tcaattgtag gtggccaccc aacatatatc tcttcattag atgttcgggt
     2461 tctaggtgtg gcaggcggtt cgatggtacg ggctaatcag tcaggaatca tcgatgtcgg
     2521 tccacgttct gcacatattg ccggtctaga ttatgcagta tttacggaga cagaaaaaat
     2581 caaaggtcct aaggttgaat ttttctctcc taaagaagga gaccctgctg attacgtcaa
     2641 ggtagtgatg gaagacggag aagaagtgac tattacaaat acatgtgcag ccaatgtcct
     2701 tggattagtg caagaagaac atttttctta tggaaatgtc ccatctgcac gaaaagccat
     2761 ccaagcatta gctgattatt gtcatacgac ggttgaagac atcgcagaac agatcatgga
     2821 aaaatcctat gctaagattg aaccggtgat tttagaattg gcagacaagt atcacttgga
     2881 gaaagatcag atttctcttg taggtgtagg gggcggcgct gcctcattga ttacttactt
     2941 tagtaataaa atgggtgtga aatattcgat tcctgaaaac gccgaagtga tttcttctat
     3001 tggtgtagca cttgcaatgg tccgagatgt tgtagaacgt atcatccctt cgccaagtaa
     3061 agaagatata cgatcgttga aaaatgaagc gatgaataaa gcgattgaat caggcgctac
     3121 accagaatcg atcgaagtcc acgtagaaat cgatccacag acttcaaaag taacggctat
     3181 cgctactggt tctacggaag tgaaagcaac cgatctaacc aaggagatca caacagaaga
     3241 agcactagag cttgctgcag aggatatgcg attaaataaa aatgaagttt gcttattgga
     3301 aaatacacct ttcttttatg tatgcggcga acagaatcgt tcaaaaaatg ctggaagtct
     3361 tcggatcatc gatcaaaaag gatttatcaa ggtccaacgt ggacatgctt catgtatgaa
     3421 aacaactgca gccaattata tgacagctgt cgaacaactt tgggaagata tggcagttta
     3481 tcaaactgaa ttgattgccc gaccagaatt ttatctctgc ttaggagcaa gagtctctga
     3541 ttttacagcg acggatctgg aacaactaca gcttttgatg gatctggaag tatctactat
     3601 ggaaccggag gaagaagtga tcgtcgttgc cggaaatatc aaacaaacat aagttagaag
     3661 agttactaaa cgaattacaa cagatccccg aagaaatttg gggattctac cagtttcggc
     3721 gagatttatt ttggaaaaag attcctttgt ccaagcagaa aattcttata caacaatcaa
     3781 tcgattgtgg tatcgaaacg gcatgtagca tcaaaaaaaa gtaccctttt gccgatgtag
     3841 gagaaatttg tgaacaaatg gctgttccaa tcgtttcctg tgaatcagag caaataaacg
     3901 aacgaatcac ttttgcaact tatgcagaag atgaagggat tcgattgatg acggagccgt
     3961 tagaaaaatt aaaatgttca ggactagctt cgatctctaa agaaacagcg caagctttga
     4021 ttattggaca tgaactattt caccacatcg aagcttctgt taagggaatt tatacgcaaa
     4081 gcgaaaaaat tgtgctgtgg cggttgcctt tttatacaca tcaatcaaac attcgtgcat
     4141 tgagcgagat agcagcgatg agtttttcaa aagagatgaa tcaaagcgat ttttcgcctt
     4201 atgtattaga ggcagtct
//
LOCUS       DACDKM010000156         4164 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20834_2016
            SAMN12640370-rid9891663.denovo.157, whole genome shotgun sequence.
ACCESSION   DACDKM010000156 DACDKM010000000
VERSION     DACDKM010000156.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640370
            Sequence Read Archive: SRR10040891
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4164)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4164)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742835.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742835.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:04:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,012
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 29 of 75
            Pseudo Genes (incomplete)         :: 33 of 75
            Pseudo Genes (internal stop)      :: 19 of 75
            Pseudo Genes (multiple problems)  :: 5 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4164
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640370-rid9891663.denovo.157"
                     /isolate="E20834_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..511
                     /locus_tag="IV334_002652"
     CDS             <1..511
                     /locus_tag="IV334_002652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phosphoesterase"
                     /protein_id="HAP8944665.1"
                     /translation="EYKNEIETCVNEIRKLNWDIVSVKKEIQDKAKVELEIEDLKKQV
                     EKLSHKSYKDVYDEYSKETDIYRELKRDVEALKEIPKNLTDSVDKIDFGNGIKVTDEI
                     DEHRGELIKNLVTNRAKIQTIINEMSDEIDSYRAELNKSDWKVNYNEVSKRYQRGIKN
                     LVKSYLKMN"
     gene            514..1410
                     /locus_tag="IV334_002653"
     CDS             514..1410
                     /locus_tag="IV334_002653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAP8944666.1"
                     /translation="MLQNLDSLYQSLSKTEERLEEIIQQEIKLKDYQVEVDSAMSRMN
                     KSYQNLYSERSSSSREIFSDITGIEATIQPNRDFNNYVKELRACINKEDTFDSEFGSL
                     IDKLTKNSITNQDIYNDILDIRVSGESSIFSDKKLINSIRKLNDAQVLELQTLKPNDL
                     VVIRLELNGKKVSLSNASAGQKTSAILSMILAYGNSPLILDQPEDDLDSQLINNLIVR
                     SIITKKENRQILIVTHNANIPVNGDSEWLISMGDSKEISADQCGSVDEPNIKPRICTV
                     MEGGEDAFTNRAKRYGFKQIVE"
     gene            1460..1849
                     /locus_tag="IV334_002654"
     CDS             1460..1849
                     /locus_tag="IV334_002654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAP8944667.1"
                     /translation="MKKIKSYTSIWAVEKVIYAINDFQLPFPVTFNQMAWFVLSLLFV
                     IVFAHVPPLSMIEEVFLKYLGIPVAVTWFMSQKTFDGKKPLGFLRSFISYHLRFKVTF
                     AGKKVKEQKKRWDEPSTLVRSVNYVSD"
     gene            1836..>4164
                     /locus_tag="IV334_002655"
     CDS             1836..>4164
                     /locus_tag="IV334_002655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HAP8944668.1"
                     /translation="MYPIKYIENNLVFNQEGECFAYYELVPYNYSFLSPEQKFQVHDN
                     FRQLIAQNREGKIHALQIATESSIRATQERSKKEITGRLKEVAKQRIDIQTDALVSMI
                     GDSQIDYRFFIGFKLIATDEEVNLKSLKKSFFSGLQEFVYGVNHHLMGDFVSLSNEEI
                     RCYSKLEKLMESKLARRFKVRRVTPSDLTYLIEHIYGEKGIPFEEYEFQLPKKKLKSE
                     TLVKRYDLLRPNRCLIEEKPRCLRMEHENHESYVAYLTINTIVGEMEFPSSELFYYQQ
                     QQFTFPIDTSMNVEIVTNKKALATVRNKKKELKDLDNHAYQSDNETNSNVLDALDSVD
                     ELETTLDQSKESMYKLSYVVRVSAESVDELKRRCDEVLDFYDDTNVKLVRPFGDMMGL
                     HEEFLPSSKRYMNDYIQYVTSDFLAGLGFGATQMLGELEGIYFGYNVDTGRNVYLKPA
                     LASQGVKGSVTNALAAAFLGSLGGGKSFSNNLLVYYAVLFGGQAVIVDPKGERGGWKE
                     TLPEIADEISILNLTSKSENQGLLDPYVIMKKKKDSESLAIDILTFLTGISSRDGEKF
                     PVLRKAIRTVTQSDQRGLLQVINELRKDGSPIAENIADHIESFTDYDFAHLLFSDGSI
                     TQSISLDKQLNIIQVADLVLPDAETTFEEYTTMELLSVSMLIVISTFALDFIHSNREI
                     FKIVDLDEAWSFLQVAQGKTLSNKLVRAGRAMNAGVYFVTQNSDDLLDERLKNNIGLK
                     FAFRSTDIHEIKKTLEFFGLDQEDQSNQKRLRDLEN"
BASE COUNT         1427 a          645 c          877 g         1215 t
ORIGIN      
        1 agagtataaa aatgagattg aaacatgtgt aaacgaaata agaaaattaa attgggatat
       61 tgtttctgtg aaaaaagaaa tacaagataa ggcgaaggta gagcttgaaa ttgaagattt
      121 aaaaaaacaa gtagaaaagc tatctcataa gagttataaa gatgtttatg atgagtattc
      181 aaaagagaca gatatttata gggaactgaa acgtgatgtg gaggcactta aggaaattcc
      241 caaaaatcta actgatagtg ttgacaaaat tgactttggc aatggtataa aagttacaga
      301 cgaaattgat gaacaccgcg gagaattgat taagaattta gtgaccaatc gggctaagat
      361 tcaaacaata attaatgaaa tgtcagatga aattgatagt tatagagcag agctgaataa
      421 atcagattgg aaagttaact ataatgaggt ttcaaagagg tatcaaagag gtatcaaaaa
      481 cttagtgaag agttatctca agatgaatta aaaatgttac aaaaccttga ttctttatat
      541 caatctttat caaaaactga agagcgattg gaagaaatta ttcaacaaga aataaagctt
      601 aaagattacc aagttgaggt tgactcagca atgtcaagaa tgaataaatc atatcaaaat
      661 ctctattctg agagaagtag ttcctcgcgc gaaatttttt ctgatattac aggaatagag
      721 gctactattc aaccaaatag ggatttcaat aattatgtta aagagctcag agcttgtatt
      781 aataaggagg acacttttga cagtgagttt ggatctctta ttgataagct gacaaaaaat
      841 tccattacta atcaagatat atataatgac atacttgata taagagttag tggggaaagt
      901 agtatttttt cagataaaaa attaatcaat tctataagaa aattgaatga tgcacaagtt
      961 cttgaattac aaacccttaa gccaaatgat ttagtagtta tccgacttga actaaatgga
     1021 aaaaaagtta gtttatcgaa tgctagtgct ggtcaaaaga catctgcaat tctttctatg
     1081 attttggcgt atggaaattc accactcata ttggatcagc ctgaagatga tttggatagt
     1141 caattaatta ataacttaat cgtaagaagt atcattacaa aaaaggaaaa cagacaaatt
     1201 ttaattgtaa ctcataatgc aaatatccca gttaatggtg attcagaatg gttgattagc
     1261 atgggagatt caaaagaaat atcggctgat cagtgcgggt cggtagatga acccaatatc
     1321 aagccaagaa tatgtactgt gatggaaggt ggcgaggatg cttttacaaa tagagcgaaa
     1381 cgctatggtt ttaaacaaat tgtagaataa cactagaggg tgagacatat tattatgtcc
     1441 gcccttttta ggaggaaaaa tgaaaaaaat aaaaagctat accagtatct gggcagttga
     1501 aaaagtcatt tacgcaatca atgattttca gttgcctttt cctgtgactt ttaaccaaat
     1561 ggcttggttc gttttatctt tactgtttgt catcgtattt gcccatgtgc ctccactatc
     1621 tatgatagag gaggtctttt taaaatactt aggcataccg gtggctgtca cttggtttat
     1681 gtcgcaaaag acgtttgatg gcaagaaacc actgggattt ttacgatcct ttatcagcta
     1741 tcacttgcga tttaaggtga cttttgcagg aaagaaagtc aaagaacaga agaaacgatg
     1801 ggatgaaccc agtactcttg taaggagtgt gaactatgta tccgattaag tatattgaaa
     1861 ataatctcgt ctttaaccaa gaaggcgagt gttttgccta ctatgaatta gtgccttaca
     1921 attactcctt tttgtcaccc gaacagaaat ttcaagtgca tgataacttt cgccaactaa
     1981 ttgcgcaaaa tcgtgaaggc aagatccatg cgttacaaat tgccacagaa agtagtattc
     2041 gcgccacaca agaacgatca aaaaaagaaa ttaccggtag actcaaagaa gtagcgaaac
     2101 aacgaattga tatacagaca gatgcattag tatccatgat tggcgatagt cagattgatt
     2161 atcgcttttt tattggcttt aaactaattg cgacagatga agaagtcaat ctaaagagtc
     2221 tcaagaaatc tttcttttct gggcttcaag aatttgtcta tggagtgaat catcatttga
     2281 tgggcgattt tgtttcctta tccaatgaag aaattcgctg ctattctaag cttgaaaaat
     2341 taatggaaag caaattggct cgaagattta aagtcaggcg agtcacaccg agtgacctga
     2401 cgtatttgat tgaacatatt tatggcgaaa aagggatacc gtttgaagaa tacgaatttc
     2461 agctaccaaa gaaaaagcta aaatcagaaa cactggtgaa acgttatgac ttattacgtc
     2521 caaatcgttg tttaattgaa gaaaaaccaa ggtgcttacg gatggaacat gagaatcatg
     2581 agtcgtacgt agcgtattta acaattaata caattgtggg ggagatggaa tttccttcct
     2641 cagaactttt ctattaccag caacaacaat ttacattccc aatcgacaca agtatgaatg
     2701 tagagattgt gacgaataaa aaggctttag ctaccgttcg taataagaaa aaagaactga
     2761 aagatttaga caatcatgct taccaatctg acaatgaaac gaattccaat gtcttagatg
     2821 ctttggattc cgtggatgaa ttggaaacca ccttggatca gtcaaaagaa tctatgtaca
     2881 aactgagtta tgtggttcga gtgagtgccg aatcagtgga tgaactaaag cgacgctgtg
     2941 atgaagtgct cgatttttat gatgatacca atgtaaaact agtccggccc tttggcgata
     3001 tgatggggct acatgaagaa ttcttaccct caagtaaacg atatatgaac gactatattc
     3061 agtatgttac gtctgatttt ttggcggggc ttggttttgg ggctactcag atgctaggag
     3121 aattagaagg gatttacttt ggctacaatg tggatacagg tcgtaatgtt tacctgaaac
     3181 cggcattggc ttcacaagga gtcaagggtt cagtaaccaa tgcgctggcc gccgctttcc
     3241 tgggttctct tggtggaggt aaatccttta gtaacaatct cttagtctac tatgctgttt
     3301 tgttcggtgg tcaagctgtg attgtcgatc caaaagggga gcgaggaggg tggaaagaaa
     3361 ccttgccaga aattgcggat gaaatcagta tcctcaactt gacgagtaag tcagaaaatc
     3421 aaggtctgct tgatccttat gtgattatga agaaaaagaa agactcggaa agtttagcaa
     3481 ttgatattct gactttttta acaggaattt caagtcgaga cggggagaag tttcctgtgt
     3541 tacgtaaagc cattcgaacg gtgactcaaa gtgatcaacg ggggttatta caagttatta
     3601 acgaattgcg aaaagacggg tcaccaatcg cggaaaatat cgccgatcat atcgagtcct
     3661 ttaccgatta tgattttgct catttgcttt tctcagacgg ctcgattacc caatcgatta
     3721 gtttagataa acagctaaat atcattcaag tggcggattt ggttttaccg gatgcagaaa
     3781 cgacttttga agaatatacc accatggaat tgcttagtgt ttcgatgcta atcgtcattt
     3841 ccacttttgc cttggacttt atccatagta accgagaaat cttcaaaatt gtcgatttgg
     3901 atgaggcttg gagttttctt caagtggctc aaggaaaaac gttatcgaat aagcttgttc
     3961 gtgctggtcg ggccatgaat gctggggtct atttcgtcac tcaaaatagc gacgatttat
     4021 tagatgaacg tttaaaaaat aatatcgggt tgaagtttgc ttttcgcagt accgatattc
     4081 atgaaatcaa gaaaaccttg gaattctttg gtttggatca ggaggatcaa agcaatcaaa
     4141 aacgcctgcg agatttggaa aacg
//
LOCUS       DACDKM010000157         4130 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20834_2016
            SAMN12640370-rid9891663.denovo.158, whole genome shotgun sequence.
ACCESSION   DACDKM010000157 DACDKM010000000
VERSION     DACDKM010000157.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640370
            Sequence Read Archive: SRR10040891
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4130)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4130)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742835.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742835.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:04:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,012
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 29 of 75
            Pseudo Genes (incomplete)         :: 33 of 75
            Pseudo Genes (internal stop)      :: 19 of 75
            Pseudo Genes (multiple problems)  :: 5 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4130
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640370-rid9891663.denovo.158"
                     /isolate="E20834_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(652..1608)
                     /locus_tag="IV334_002656"
     CDS             complement(652..1608)
                     /locus_tag="IV334_002656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8944669.1"
                     /translation="MLILANQASTFVQTQQLRGRGLRLGAQNKVTNIWHLAIGLPGVP
                     LSEQPDLKRMLRRLGNISGLTFESSPKIESGGDRFALPLEYTTTTMETYTQSMFYFSC
                     ERSRLKEQWSTALADGWHLSMPIVVRKVDQVKQKQETSLPIVVKNIEPLSFWSAIFKG
                     NLLEYFQIRRRQKLWEKECARKKSLAICVQTTLQRLGEVSDRKVIVAADQQEFRCYLE
                     NAGYREGQLFTEAMRELLAPLENPRYLIEGKKIVLTVPERFGRNKQIANLFLNELNQS
                     SLPKCQLVYTRNPEGRQKLVTYRMRQVFQTRDEVIIQKNIWT"
     gene            complement(1717..1962)
                     /locus_tag="IV334_002657"
                     /pseudo
     CDS             complement(1717..1962)
                     /locus_tag="IV334_002657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347587.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(2107..2529)
                     /locus_tag="IV334_002658"
     CDS             complement(2107..2529)
                     /locus_tag="IV334_002658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8944670.1"
                     /translation="MVKEEVLCPHQDFLYLIPANQETTQHYFTFLEKQNELVEALMES
                     QETVDYLLSLEFVQHPLEHSKEIYENFELFTSALIYLNHHNYELIPEHWQLLGSSKQQ
                     GKKASVPSLGKKELITIYQQMFEDQPELPIFYQLKKAG"
     gene            complement(2668..3098)
                     /locus_tag="IV334_002659"
                     /pseudo
     CDS             complement(2668..3098)
                     /locus_tag="IV334_002659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347687.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
     gene            complement(3414..3677)
                     /locus_tag="IV334_002660"
     CDS             complement(3414..3677)
                     /locus_tag="IV334_002660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8944671.1"
                     /translation="MFIKYGADTVATMFTEEYQVDYYEVIDKCVHFVYEKVWNFMNFA
                     ISEEILRVQENRTGIFTRDRKLNLIAHYIRNRWSSIPNFNYKK"
BASE COUNT         1263 a          852 c          635 g         1380 t
ORIGIN      
        1 gcagactgat aatagcaaaa gaaaacttat aatcaaataa tatcaacatt tgatcacata
       61 atttgagatt gaatgctgat ttacctatgt tttacgtaat ggtctttagt tatttttgct
      121 cataaaatgc ataaaacacg cgtttttatg tgtgcttgaa atttcttggc tctataattt
      181 ctaagactga taaaaatatt ctatcggttc tttttgcaaa taaaaaaaca tatagaagct
      241 ccattagatt gaatcaccac aacacaatca aaggagcatt ccgtatgctt gattttcatt
      301 ctataaaact acttttagga atcaaagaaa atcacaacgg ttcaaactaa aagcaagaat
      361 atccgaagta ccattgtttc atcgatcatg agaaaagcat tcacctctgt ctgcagatgt
      421 tcttatatcg ctctgtcagt cctattaata aagtactgtt ttgttttcaa aaaaactatt
      481 acgagtactt tttaagagaa tgccttgcaa atattcatca cacaccgtta tataagtcaa
      541 atagtttctt tcagtcgtca agaaaagatt ctcctgtcct tggcattatc agttaagtag
      601 caacttaatt ctcggacttg ttctactcta gcaaaattta ttttcatcaa cttacgtcca
      661 aatgtttttt tgaataatta cttcatcgcg ggtctggaag acttgcctca ttcgataagt
      721 cactagcttt tggcgtcctt caggatttct agtatacacc aactgacatt tgggcagcga
      781 cgattgattt aattcgttta gaaaaaggtt agcgatttgc ttatttcggc caaagcgttc
      841 tggtaccgtc agaactatct ttttcccttc gattaaatac cgcggattct ccagcggtgc
      901 taaaagctcc ctcatagctt cagtaaaaag ctgtccttct cgatagccag cattctcaag
      961 atagcatcta aattcctgct gatcagccgc tacgattact tttctatcac taacttcacc
     1021 aaggcgctgc aacgtcgttt gaacgcatat ggccaagctt tttttgcggg cacattcctt
     1081 ttcccatagc ttttgtcgcc tacgaatttg gaaatattcc agcaagttac ctttaaatat
     1141 agctgaccag aagcttagag gctctatatt ttttactact atcggcaatg aagtttcttg
     1201 tttttgcttt acctgatcga ctttcctaac gacgattggc atagataggt gccagccatc
     1261 agctaaagca gtactccatt gttctttcaa ccgacttcgt tcacaagaaa aatagaacat
     1321 cgactgagta taagtttcca tcgtggtggt cgtatattcc agtggtaaag caaagcgatc
     1381 gccaccactc tcaatttttg gtgaagattc aaaggtcaga cctgaaatat tgcccaaacg
     1441 ccgtagcatt cttttcaaat caggctgttc agacagagga actcccggta atccaatcgc
     1501 taaatgccaa atgttcgtta ctttattttg tgcacctagg cgtaagcccc gccctcgtag
     1561 ttgttgtgtt tgcacaaaag tggaagcttg attagccaag atcaacagat taattgcagg
     1621 acaatcccag ccttctccta gcaaactaac ggttcctacc aaaatattca acttcttttc
     1681 ggttaatagg cgagtaactg cttgcatcaa tgcttggcgg tttttgtcat ccaccgtcaa
     1741 ataaaaataa cccgacatat aaggatgttc attcataccc gtcgctagtt ctgccagctc
     1801 aacagttact agcattttcg gtaacagtaa gaactctcca cagatggcac cgatttgctg
     1861 agtgttacct aatagacgaa caacttcatc aaacaaagga accacgccat aagctaaggt
     1921 accatcactg cctgctaata cctccttgca aatacgatcc atcaaaatga ccgctgtcag
     1981 ttcttgcttc agctgaactt cttcttttaa caaaatttga caaactgctt gctttttctc
     2041 aggaatcgcc tgactaccaa cagtgtctgg atactctgga aacaaatgca gcgactcatc
     2101 aaacaatcat ccagcttttt tcaattgata aaaaataggc aattccggtt gatcttcaaa
     2161 catctgctga taaatagtga tcagctcttt tttacctaaa gatggaactg aagctttttt
     2221 accttgctgc ttagaactgc ctaatagctg ccaatgttcg ggtatcaatt catagttatg
     2281 atgattcaag taaattaatg cactagtgaa cagctcaaaa ttttcataaa tttccttcga
     2341 atgctcaagc ggatgctgaa caaattctaa agaaagcaaa taatccaccg tctcttggct
     2401 ttccattaac gcttccacca gctcattttg tttctctaaa aaggtaaaat aatgctgagt
     2461 agtttcctga ttagccggaa tcaaatatag aaaatcctga tgaggacata gaacctcctc
     2521 ctttaccaac tcgggaatgc taatttcttc gtcaatcagg ccattttaac tcaatatatt
     2581 gcttccactc cgtctgagag ctatcaatcg gaggcgtggc tgtcaaggaa atcgtcaaca
     2641 gattcggttg ttttttcttc aagtcaatca acaattcacc ccagcttttt tttaaatgat
     2701 gagcttcatc aaaaaactaa cagctccagc tgatcaccat aagttggctc ctccttcagt
     2761 cggttgtaaa tggtctgata cgtttccact gtaatatgac taggatgttc aatcgcccca
     2821 gaaagatagc aactatctgc tggatttact aagaaatatt cctcgattgt tgttaaccac
     2881 tgtttcttca atattaggga aggaaccaaa attaaggttt tacaatccaa acgtcggatc
     2941 atctcaattc ctaaaaaggt cttgccagcc cctggtggag ccactagatg gactgaattg
     3001 ttatttaaat aagtagcaaa gttagtcaat atacgcttct gatagctacg ccactgaatt
     3061 ttttcatcag atagccacat cccaggtccc cctctcacgt attttctcta tcataatcta
     3121 aatgaataca gcaaaaaaaa gatttcttca agaatatgtg tttttatagt aatattttac
     3181 ttactctaaa ctcaaatgaa aaggtaaaaa aactggtagt acaatctttt aaattctcat
     3241 tgttttgtac gttctttact atcaaaaaaa caaaaaaaac gcgtttctac gcgtgaccaa
     3301 aatttttagc tctataattt ctaagactga tagaaaatca ttctgttttt gaagtctttt
     3361 ttcataaata ctacttgatt tattgtgtat attgtataaa agattgttca taattatttt
     3421 ttataattaa aatttggtat tgatgaccag cgattccgta tataatgcgc aatcaaatta
     3481 agctttctgt ctctggtaaa tattcctgtt ctgttttctt gtactcgtaa gatttcttca
     3541 cttatcgcaa aattcataaa attccacact ttttcataaa caaaatgtac acatttatct
     3601 attacttcat aatagtctac ttgatattct tctgtaaaca tggttgctac tgtatctgca
     3661 ccatatttga taaacatttg ggctactccc ttaaattgag aaaagcccat tagtttatct
     3721 actaatactt ctaaaatttc ttgctttctt ctttaacatc gtcccgtaag gaatcatgta
     3781 gtatgctgga ggaagttcat tttctaaata attgtatcgc ttcaattgct caaaggctat
     3841 tctctacaat taattacacc aattacttta tcttcactta tataagattt ctaaacattt
     3901 ttcgtcaaat gaagagaaaa tattttttta aatgatcttg ctcaagattt gtcaacgaat
     3961 ctttgaattc gttttgaagt tctgaggaag gtatcattta aaaactcaat ctgactttca
     4021 tcatttcgtt tactcccatt ttgatcacct tctaccaaca atttattaaa gtgattatac
     4081 taggttttta tttttttaat tcacaattgt ttattcactt catgaacaat
//
LOCUS       DACDKM010000158         4041 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20834_2016
            SAMN12640370-rid9891663.denovo.159, whole genome shotgun sequence.
ACCESSION   DACDKM010000158 DACDKM010000000
VERSION     DACDKM010000158.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640370
            Sequence Read Archive: SRR10040891
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4041)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4041)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742835.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742835.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:04:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,012
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 29 of 75
            Pseudo Genes (incomplete)         :: 33 of 75
            Pseudo Genes (internal stop)      :: 19 of 75
            Pseudo Genes (multiple problems)  :: 5 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4041
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640370-rid9891663.denovo.159"
                     /isolate="E20834_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(64..606)
                     /locus_tag="IV334_002661"
     CDS             complement(64..606)
                     /locus_tag="IV334_002661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2179 domain-containing protein"
                     /protein_id="HAP8944672.1"
                     /translation="MIDPKMLGMIFLINFAYITLNTLRFMLTMKGYRLIAPLVSMVEI
                     TIYIVGLSLVLDRLDNPLNLFVYALGYAVGISVGILIEDKLALGYIMVTTILPANSAE
                     DKSLPGILREHGYGVTQTSAMGLEGERLVLEILSPRKSERELYRLIKEIEERAFIISY
                     EPKYISGGFWTKKVRKRQGK"
     gene            741..1130
                     /locus_tag="IV334_002662"
     CDS             741..1130
                     /locus_tag="IV334_002662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8944673.1"
                     /translation="MKTVNRVFCASHVLSLLLSIYLGVYSIQQPIEVENIVFQMSLTD
                     GLILSVFFFLILFIGNIFGSVASFLNFSIYPLLSLALGVVGLCSLCLFPEPFSPAFIL
                     FGTLNLFQTTVGAWLLWRSGNLMKIGE"
     gene            1134..1898
                     /locus_tag="IV334_002663"
     CDS             1134..1898
                     /locus_tag="IV334_002663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HAP8944674.1"
                     /translation="MNPIILKRSVHHIPVLEVVLKELREERIPVVIYYHGWQTSKELV
                     LTQGRKIAQQGIRVILPDAMNHGERKQPVSSIPSYTFWDSIYGNLFEFDTLIKHLEKR
                     GLLHDQLAVGGVSMGGMTTCALLAKHPHIVAGVCLMGSPAPLEYGKEIVRRASEYNYV
                     VPKDYFDLISWVKSYDLSLQPEKLAGRPLFFWHGQADEKIPYIQTERFVEKNRHKSYG
                     KEIVFRSSKTQRHMVQIPLMEEASTFLAKNLIKKYK"
     gene            2027..2974
                     /locus_tag="IV334_002664"
     CDS             2027..2974
                     /locus_tag="IV334_002664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAP8944675.1"
                     /translation="MPTLADVAKRANVSKMTVSRVINHPEQVTDELKELVFSAMAELD
                     YRPNIAAKALVTNRSQIIKLFILEEIDTTEPYYMNLLMGIAKRIGKAHYSLQLVTNDA
                     FDVGSCDGYIITGVRKKDFDWIDRLEKPVVLFGENSEGYDYVDSDNEYGTAKATQYAL
                     ERDYQTIIYIGIEVDEPFEISRETGYLSVMKEVDLPSKILRFPNHSTEVERYMTENWA
                     DFTDKTCFVCSSDRLALGAVRGIINAGGHLPEEFGVIGFDGVFLDQISSPKLTTIKQD
                     IIRMGEVCGEMLLKKIEEKGASQGYRRFFPELVIRETTN"
     gene            3104..3844
                     /locus_tag="IV334_002665"
     CDS             3104..3844
                     /locus_tag="IV334_002665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8944676.1"
                     /translation="MKEIRRKHFRFPAYDDELGVKLTNDKKRVLFDGQEDLLTEQNQS
                     PFEQMKEFHFTNTNTEKIRRKRRATNGLKSAPRKENLEKHKGKLPDYTGHYQSEITST
                     GRKKLFGDSSRSVRQSETRTESKHTTRNASVDTKVRERSYFVPKYVPASIIPDPRESK
                     ISEDHLIESMKKTDDSYLLFDTEPTPYQEKRNGEPSVRTFNRTKTLEETKQSEEKKSK
                     KSHGILERSLQGLIDDQGNSLGENSYFK"
BASE COUNT         1346 a          687 c          815 g         1193 t
ORIGIN      
        1 aaataaaaac tgtctaacat ttaaaattta atttagatat tagacagttt ttaagtttgt
       61 gagttatttt ccttgtcgtt ttctcacttt tttagtccag aaaccacctg aaatatattt
      121 tggctcataa gaaatgataa aagcacgctc ttcgatttct ttgatcaggc gatacagttc
      181 acgttcgctt tttctaggag aaagtatttc gagaactagc cgctcacctt ccagccccat
      241 tgcagaagtc tgtgtcacac cataaccgtg ttctctcagt atacccggca aacttttatc
      301 ttctgcgcta tttgctggca aaatcgttgt gaccatgatg tatcctaatg ccaatttgtc
      361 ttcaatcaaa attccgacac tgatacctac tgcgtaacct aacgcataga caaataaatt
      421 cagtggatta tctaaacgat ctaaaacaag ggataagccg acgatataaa tcgtgatctc
      481 gaccatactg acaagtggag caatcagtcg atagcctttc attgtcaaca tgaaacgtaa
      541 cgtgttcaga gtgatataag caaaattaat gaggaagatc atccccaaca tttttggatc
      601 aatcatttgt aaaactcctt tcatttatat aacaacaata tcaaaaaaaa tcgtaagtga
      661 agaaaaattg aaactttata catacaaaaa aatgataaaa tgattataat agaattataa
      721 agataggtta ggtgattaaa atgaaaacgg tcaatcgtgt gttttgtgcc agccacgtgc
      781 taagtctcct tctcagtata tacttaggtg tctattcgat tcaacagcca attgaggtag
      841 agaacattgt ttttcagatg tcgctgacag atggtttaat tttaagcgtt tttttctttt
      901 tgatactgtt tattggtaat attttcggta gcgttgccag ttttttgaat ttttctattt
      961 atccactcct ttcgctagca ttaggagtgg tgggactttg ttctctgtgc ttatttcccg
     1021 agccattcag tcctgcattt atattatttg gtacactgaa cctgttccag acaactgttg
     1081 gagcatggtt gctatggcga tcaggaaatt taatgaaaat aggtgagtaa caattgaatc
     1141 caattatatt gaaacgttct gtccatcata ttccagtgtt agaagttgtt ctgaaagaat
     1201 taagagagga acgtattccc gttgtcatct attatcacgg atggcagaca agtaaggagt
     1261 tagttttgac gcaaggaaga aaaattgcgc agcagggcat tcgtgtgatc ctgccagatg
     1321 cgatgaatca tggggagcgc aaacagccgg tttcttctat tccatcctat acattttggg
     1381 atagtatcta cggaaatctg tttgaatttg acacacttat caagcattta gaaaaaagag
     1441 gtcttctaca tgatcagctt gcagttggcg gggtttcgat ggggggaatg acaacatgcg
     1501 ccttgttggc taaacatcct catatagtag cgggcgtgtg tttgatgggt tcccctgcac
     1561 ctttggaata tggaaaagaa atcgtaaggc gcgcatcgga atataattat gtcgtaccaa
     1621 aagactattt tgatttgatt tcatgggtta agagctatga tttgtcattg cagccggaaa
     1681 agttagctgg acgtcctctg ttcttttggc atggacaagc tgacgaaaaa atcccttata
     1741 tacaaacgga acgatttgta gaaaaaaatc ggcataagtc ttacggaaaa gaaattgttt
     1801 ttcgttcctc aaaaacacag cgacatatgg tacaaatacc actaatggaa gaagcttcca
     1861 cttttttagc aaaaaatttg ataaaaaagt ataaataatt gaaacggaaa atcactcatg
     1921 acgatagagg attttccgtt ttttattttg ggctataata aactctgtta ggaaaatttg
     1981 gtataattga tagtaagaat gacaacatgt tagaaaggaa gagagcatgc ccactttagc
     2041 agatgttgca aaacgtgcca atgtttcaaa aatgacggtc tctcgagtca tcaatcatcc
     2101 ggaacaggtg actgacgaat tgaaagagtt agttttctca gcaatggctg aactggatta
     2161 ccgtccaaat attgctgcaa aagcacttgt cacgaatcgg tcgcagatca tcaaattatt
     2221 tatactggaa gaaatcgaca caactgagcc ctattacatg aatttgctga tgggaatcgc
     2281 taaacggatc ggtaaagcgc attattctct ccaattagtt acaaatgatg ccttcgacgt
     2341 cggttcttgt gatggataca tcatcacagg tgtcagaaaa aaagacttcg attggatcga
     2401 ccgtttggaa aaaccagttg tcttgtttgg ggaaaattct gaaggatatg attatgtaga
     2461 cagcgataat gaatatggta cagcaaaagc aacgcaatat gccttagaaa gagattatca
     2521 aacgattatt tatatcggta tcgaagtgga cgagcctttt gaaatttcaa gggaaactgg
     2581 ttatttatct gtgatgaagg aagttgatct tcctagtaaa atcttgcggt ttccaaatca
     2641 ttcgacagaa gtagaaagat atatgacaga aaactgggca gattttacag ataaaacttg
     2701 ttttgtatgt agttcagatc ggctagcact aggagcagta agagggatta taaatgctgg
     2761 aggacacttg cccgaagaat ttggtgtgat tggatttgat ggggtatttt tagaccaaat
     2821 cagttcacct aaattgacaa cgatcaagca agatattata cgtatgggtg aagtatgtgg
     2881 agaaatgttg ttaaaaaaaa tagaagaaaa aggggcgagt caaggttatc gtcgtttttt
     2941 tcctgaacta gttatcagag agacaacgaa ttaagtctta ttactatata tatatttatg
     3001 aaattcatcg ctattttctt aaaataatca gcgcattgct actgaaaaca atgaatctgt
     3061 gctattattg agaaaagttc catttttgag aaggatggtt tgaatgaaag aaatcagacg
     3121 aaaacatttc cgttttcctg cgtacgatga tgagttaggc gtaaaattaa ccaacgacaa
     3181 gaagcgagta ttatttgacg gacaagaaga tcttttgact gaacagaatc agtccccgtt
     3241 tgaacagatg aaagaatttc atttcacgaa cacgaacacc gagaaaatcc gtcgcaagcg
     3301 tcgtgcaaca aatggattga agtcagctcc gcgtaaggaa aatttggaaa agcataaagg
     3361 aaaattaccg gattataccg gccattacca atcagaaatc acatcgacag gacgaaaaaa
     3421 actttttggt gattcctctc gttctgtcag acaaagtgaa acaaggacag agtccaagca
     3481 tactacacga aacgcatctg ttgacacgaa ggtgcgggag cgttcttatt ttgttcctaa
     3541 atatgtaccc gcatcgatta tcccagaccc tagagaaagc aaaatttctg aggatcattt
     3601 aatcgaatcg atgaaaaaaa cggatgactc ttatctgttg ttcgatacag aaccgacgcc
     3661 gtatcaagaa aaaagaaatg gtgagccgtc tgtacgaaca ttcaatcgaa caaaaacgtt
     3721 agaggaaaca aagcagtcag aagaaaaaaa atcgaaaaaa agccatggta tcttagaacg
     3781 ctcattacaa ggattgatcg atgatcaagg aaactctttg ggagaaaata gctattttaa
     3841 ataagtgata tacaaattta tattcggcta cagttggaaa ggaaacgctg cggcgtttcc
     3901 ttattttata ctctatccga aagtttatat cgtatcctca aatttttttg agtttaagcg
     3961 aattttttct cccaataagt aagaaaataa tggcaaaata aaaaaacaaa ctattttcgc
     4021 agatcgtttg caaaaatagt t
//
LOCUS       DACDKM010000159         4029 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20834_2016
            SAMN12640370-rid9891663.denovo.160, whole genome shotgun sequence.
ACCESSION   DACDKM010000159 DACDKM010000000
VERSION     DACDKM010000159.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640370
            Sequence Read Archive: SRR10040891
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4029)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4029)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742835.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742835.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:04:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,012
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 29 of 75
            Pseudo Genes (incomplete)         :: 33 of 75
            Pseudo Genes (internal stop)      :: 19 of 75
            Pseudo Genes (multiple problems)  :: 5 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4029
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640370-rid9891663.denovo.160"
                     /isolate="E20834_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            121..513
                     /locus_tag="IV334_002666"
     CDS             121..513
                     /locus_tag="IV334_002666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8944677.1"
                     /translation="MLDDRLTCSNCGTLFYYEEVRNIVKHRKKEMPIRCPNCNEVIKR
                     KLSHGYFVSYELYEESKINLKKKPKYYKNLKTGAIITEKEFKNLVTEEESRFYNLSGN
                     ICPGFMDQIIENESIESIENYVPVDLPK"
     gene            complement(686..868)
                     /locus_tag="IV334_002667"
                     /pseudo
     CDS             complement(686..868)
                     /locus_tag="IV334_002667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324322.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
     gene            855..1052
                     /locus_tag="IV334_002668"
     CDS             855..1052
                     /locus_tag="IV334_002668"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8944678.1"
                     /translation="MKDSIVEPEGMENTFELYNKTTVDKLIPILVPTDLNGHDVLLRF
                     NGFLGEDYRYLKISDPQPLTE"
     gene            complement(1095..1670)
                     /locus_tag="IV334_002669"
     CDS             complement(1095..1670)
                     /locus_tag="IV334_002669"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HAP8944679.1"
                     /translation="MAQLFYRYGTMNSGKSIELLKVAHNYEEQGKRVIIMTSAIDTRS
                     GFGKVESRIGVSKPAIAITSEMDVYGELVEIIDQYGKVDCILVDESQFLSRENIIDLS
                     LIVMNEEVPVICYGLKNDFQNNLFKGSEALLTFADKIEEIKTICHFCNKKAVMNARIV
                     DGIPVYSGEQIVVGGNEDYLSVCRNHYYNED"
     gene            complement(1825..2351)
                     /locus_tag="IV334_002670"
                     /pseudo
     CDS             complement(1825..2351)
                     /locus_tag="IV334_002670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324322.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
     gene            2808..>4029
                     /gene="gadC"
                     /locus_tag="IV334_002671"
     CDS             2808..>4029
                     /gene="gadC"
                     /locus_tag="IV334_002671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_465885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate:gamma-aminobutyrate antiporter"
                     /protein_id="HAP8944680.1"
                     /translation="MNKKKLSLFGFFALTASMVLTVYEYPTFATSQLHLVFFLLLGGI
                     LWFLPVALCAAEMATVEGWKDGGIFSWVSQTLGERFGFAAIFFQWFQITVGFITMIYF
                     ILGALSYVINFPELNTDPFIKFIGVLVIFWVLTFSQLGGTNRTAKIAKAGFVIGIVIP
                     SIILFGLAAAYFIGGNPIQMAINAKAFIPDFSQLSTLVVFVSFILAYMGVEASASHIN
                     ELENPQRNYPLAMFLLVILAIALDAIGGFSVAAVIPQHDLSLSAGVIQTFETLILHFN
                     SHLHWLVKVIALMIACGVMGEVSSWVVGPSRGMFAAAQRGLLPVFFRKTNKQDVPVPL
                     IMIQGIIVTIWAAVLTFGGGGNNLSFLVAISLTVVIYLVGYLLFFLGYFVLVFKKKDL
                     KRTYNVPGKTVGKTI"
BASE COUNT         1225 a          676 c          694 g         1434 t
ORIGIN      
        1 ttttttcttt taagtaaaaa atataggtga tttttttgac ttcatattta taataaagac
       61 acgtaaatca attatttttc attttacttt aactaataat gtttatttgg agggattatt
      121 atgttagatg atagattgac gtgttctaat tgtggtacat tattttacta tgaggaagtt
      181 agaaatattg taaaacatag aaaaaaagaa atgcctatac gttgtccaaa ttgcaatgaa
      241 gttattaagc gaaagttgtc tcatggttat tttgtcagct acgaactata tgaagaaagt
      301 aaaataaatc tgaaaaaaaa accaaaatac tataaaaatt tgaaaactgg agctattata
      361 actgaaaaag aatttaaaaa tttagtgact gaagaagaaa gcaggtttta taatttgtct
      421 ggtaatattt gccctggatt tatggatcag attattgaaa atgagtcaat tgaatcaatt
      481 gaaaactatg ttcctgttga cttaccaaaa tagaaaaaat aatcctgttt ttacagtgaa
      541 ttattttaat tttgattttc aataaaaata tattacaata ataatcgtaa tagctaatga
      601 aacaatttct caatcttttt accactccaa acagggagcg tcaataattt tgtgtaaata
      661 actcgtcctt ctgcaaaata atgggttact cagtaaacat tgaagctaat gtatccgtca
      721 cttgttggaa gcctttatgg cttctattta agaatttttg attgtatgta tcaaagatgg
      781 atactaggaa acgctctagt gattcttcat tttgaaactg ctcttttctg cggctgtatt
      841 tcttgatttg tttattgaaa gattcgattg tagaaccaga aggaatggag aacacttttg
      901 aactttacaa caaaactact gttgataaat taattccaat tttagttcca actgatttaa
      961 atggtcatga tgttttactt cgttttaacg gatttttagg tgaggattat agatacctaa
     1021 aaatttctga tcctcaacct ttaacagaat aataaaaaac gtgtaatcaa ttatttgatt
     1081 acacgttttt tatttcaatc ttcgttgtag tagtggttgc gacataccga aaggtaatct
     1141 tcattacctc ctactacaat ctgttcacct gaataaactg gaataccatc aacaatacga
     1201 gcattcatta cagccttttt attgcaaaaa tggcaaatag ttttaatttc ctcaatttta
     1261 tcagcaaatg tcaataatgc ttcggatccc ttgaagagat tattttgaaa atcattcttc
     1321 aatccatagc aaattactgg tacttcctca ttcattacga taagtgatag atcaataata
     1381 ttctctctac ttaaaaattg agactcatcg accaaaatac aatcaacttt tccgtactga
     1441 tcaataatct ccactaattc tccgtacaca tccatttcag aagtaattgc aatagctggt
     1501 ttcgaaacac ctatacgact ctcaactttt ccaaatcctg atcgagtatc tattgcgctt
     1561 gtcattatga taactctctt cccttgttct tcataattat gagccacttt cagtagctca
     1621 atactctttc cactattcat tgttccatat ctgtaaaata attgtgccat taaatttatt
     1681 cccttctttt acaattcaat tattccttaa ttccatctgc ttttatgcag ctgctcgttt
     1741 attccttgat atgtctataa catctactat ccctaaaacc aatttataag ccattagaga
     1801 tggtattcct gaacatctct catattgttt tttgagtggt atatcatctg aaaaactgaa
     1861 ggaatcatga aaatagcgac atacttatca tttagagttc taactttaaa tgcatttact
     1921 tcttcagtca gcactttagt catgttggac atggtttggg gcgtgtagtg atgcccatac
     1981 attttctcaa tcaagtctgc gatttccgac atggtaatac cttttcggaa gaggtgaatg
     2041 acggtctcct ctaatgtgtc attcgtccgt ctataagcag gaacagtctg ttgcttgaac
     2101 tcgccattgc ggtcgcgcgg aatctggaga tgaagttccc catactcggt cttgaccgta
     2161 cggtcatagg agccgttacg agaattaccc gtgttaaaac caatgcgatc gtacttttcg
     2221 taatcgagga aagccgttaa ctccgttttg agaagcgtat tgacagcttt ttccaaatgg
     2281 acacggaata atccattcaa atcgcctttg ttcgctagag tctttaggat ttctgtagta
     2341 aaatcgttca tatggaagtc ctcttttctg tgaatgtgtt gtggtaactc tattctacag
     2401 aagggacttc cttttttcta tttacacaaa atattttaca ctctcaacaa aagtatcttc
     2461 gattaatact tgctcaagtt atgtgcagct acgaaaaagt aggtaccgag gtactttctt
     2521 tttcgcagct ttttttggac ttatgagaag tacattcgct ggaagaatac gatataaaac
     2581 gagctttttc actaaagata ttaatagagt gaaagaaaca agcttttgtt gtcagttttt
     2641 tcacttttct aaaaatgaaa ataaaaaatt ctggaagtgg agaagaattt tgcttgctat
     2701 actttgtctt gtagaaaaga ggtggacaaa ataatgcaag ctcaaataag cgattttcca
     2761 taaccttggt aggaatgaca aattaaatag gaaagggaag taataatatg aataaaaaaa
     2821 agttatctct attcggtttt tttgccctga cagcttccat ggttttgact gtttatgagt
     2881 atccgacctt tgcaacatca caattacatt tggttttctt tcttcttttg ggaggtattt
     2941 tatggtttct accagttgct ttatgtgctg cagaaatggc aacagttgaa ggatggaaag
     3001 atggtgggat tttcagttgg gttagtcaaa ctttaggaga acgttttggt tttgcagcta
     3061 tttttttcca atggtttcaa attacagtag gttttattac aatgatctac ttcatactag
     3121 gagcgctgtc gtatgtaatt aattttccag agcttaatac agatccattt atcaagttta
     3181 ttggtgtatt agtcattttc tgggtactga ctttttcaca gttgggcggg accaatcgta
     3241 ccgctaaaat tgctaaagca ggatttgtca ttggaattgt tattccttcg atcattctct
     3301 ttggtttagc agcagcctac tttatcggcg gtaatccgat tcaaatggct attaatgcga
     3361 aagcttttat tcctgacttt tctcagcttt caactttagt agtatttgta tcttttatat
     3421 tggcttatat gggcgtagaa gcttcagcat cacatattaa tgagctggaa aatccccaaa
     3481 gaaactaccc attggcaatg tttttactag tgattttggc catcgcctta gatgcaatcg
     3541 gcggtttctc agtagcagcg gttattccac aacatgactt gtctctcagt gctggtgtaa
     3601 tccaaacatt tgaaacgctt attttacatt ttaacagtca tttacattgg ttggtaaaag
     3661 tgattgctct catgattgcc tgtggcgtta tgggtgaagt tagttcttgg gtagttggtc
     3721 catcacgagg gatgtttgca gctgctcagc gaggtctgtt accagttttc ttccggaaaa
     3781 ctaataaaca agacgttcca gttccgttga ttatgataca aggaattatc gttactattt
     3841 gggcagcagt cttaactttt ggtggaggag gcaacaattt atcattctta gtagcaatct
     3901 ccttaactgt tgtcatttat ctcgtaggat atttactatt tttcctaggt tactttgtat
     3961 tagtttttaa gaaaaaagac ttaaaacgaa cttacaatgt accaggaaaa actgttggca
     4021 aaacgatta
//
LOCUS       DACDKM010000160         3940 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20834_2016
            SAMN12640370-rid9891663.denovo.161, whole genome shotgun sequence.
ACCESSION   DACDKM010000160 DACDKM010000000
VERSION     DACDKM010000160.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640370
            Sequence Read Archive: SRR10040891
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3940)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3940)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742835.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742835.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:04:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,012
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 29 of 75
            Pseudo Genes (incomplete)         :: 33 of 75
            Pseudo Genes (internal stop)      :: 19 of 75
            Pseudo Genes (multiple problems)  :: 5 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3940
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640370-rid9891663.denovo.161"
                     /isolate="E20834_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            421..654
                     /locus_tag="IV334_002672"
     CDS             421..654
                     /locus_tag="IV334_002672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="addiction module antitoxin"
                     /protein_id="HAP8944681.1"
                     /translation="MAIKERKFREVGNSVVLTISKEFLKENGLNPGDTVFLDEEKLRA
                     AITKKQALSDEIDLAISQALTEYDTTFRELVDK"
     gene            651..1058
                     /locus_tag="IV334_002673"
     CDS             651..1058
                     /locus_tag="IV334_002673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system death-on-curing
                     family toxin"
                     /protein_id="HAP8944682.1"
                     /translation="MSIHYLEAKDIIKMNVIQIKKYSPNEPLGVKDANALEMCVGQLR
                     ASAFGEEVYPSIYEKAAILLIQLIKKHPFHNANKRTAFLATFVFLKINGQLLAIDQKD
                     AVNLAVYIATYDKDFDQLKYEVINTIKTHTQPI"
     gene            complement(2195..2713)
                     /locus_tag="IV334_002674"
     CDS             complement(2195..2713)
                     /locus_tag="IV334_002674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013852041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClbS/DfsB family four-helix bundle protein"
                     /protein_id="HAP8944683.1"
                     /translation="MRTYKNKEELKKEINRTYTKYILEFETIPEELKDKRVEEVDRTP
                     AENLAYQVGWTNLILKWENDERNGLSVKTPSDQFKWNQLGELYKWFTNTYAHLPLKEL
                     EEILNRNIDDINMMIDSMSDEDLFTAHKRKWADEATKTAVWEVYKFIHVNTVAPFGTF
                     RTKIRKWKRLAL"
     gene            3831..>3940
                     /locus_tag="IV334_002675"
     CDS             3831..>3940
                     /locus_tag="IV334_002675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAP8944684.1"
                     /translation="MSKRTRRTFSQEFKQQIVNLYLAGKPRVEIIREYELT"
BASE COUNT         1357 a          648 c          603 g         1332 t
ORIGIN      
        1 ctcacgatct tgaacaaaat cttttatata atcgggcata tgaagtatga ttgtatctat
       61 tttttgatat atttctgtat ttttcatgtc attcatcctt tcaataagta ggggagaaga
      121 gtatctctat aattggacta taccaattat agaatgtttt gtcaattggt atgctctaaa
      181 atttattaat ttgtttcaaa aaaagcatat aaacaggtat acgtttaata atttaaaata
      241 aatggtatac ataaataggg aaggaaatag aagggagcaa tctaaatttt ttgaattttc
      301 ttaattaatt atatagaaag aacaatcttt ctatagagtt agacatgaga gggactatgc
      361 ttgatttaaa atcaacaagc gtatatactg tatatacaaa gaaaaaggag ggagaagact
      421 atggcaatta aagaaagaaa atttagggaa gtggggaact cagtagtatt aactatttca
      481 aaggagtttc ttaaagaaaa tgggttaaat cccggagata cggttttttt agatgaagaa
      541 aaacttcgag cggcaattac aaaaaaacaa gccttatctg atgaaattga tttagcaata
      601 tctcaagcat taactgaata tgatactact tttagagagc ttgtcgataa atgagtattc
      661 attacctaga agccaaagat attataaaaa tgaatgtaat ccaaataaaa aaatactctc
      721 ctaatgaacc ccttggtgta aaggatgcga atgctttaga aatgtgtgta gggcaacttc
      781 gagcctcagc ttttggtgaa gaggtttatc cttcgattta tgaaaaagca gctatcctac
      841 ttatacaatt gataaagaaa catccttttc acaatgcaaa taaacgtact gctttcttag
      901 ctacttttgt ctttttgaaa atcaatggac agcttttagc tattgatcaa aaagatgctg
      961 taaatttagc ggtatatatt gctacgtatg ataaggattt cgatcaacta aaatatgaag
     1021 tgatcaacac tataaagaca catacgcagc ccatttaagg agttactgta tgtgtcttta
     1081 tagggttatt agctgctata gcgaaataat tgaagtaaat tacaaatagc tataattcaa
     1141 atccaactat atagaaaacg taaattttct atatagttgg attaagataa cgactattta
     1201 ttttatcata tttttactat accctatact tttacttggt atatttatga ataatcgcaa
     1261 atttacccct atactactag tttgtaagtt agattaataa aaatactaaa gttatttgtt
     1321 cttttattaa atacacctac aacttataaa ttgatggtat ttagataaca cgaaagaata
     1381 aaaataacag ctgatagtaa agtttgttga cgattgctac tcaatcgaaa gtgagttagt
     1441 gcgtatgaac ttgcttctga aaacttatga aatgaaagac cttttacatc ttgcagaaat
     1501 cggtactggg attaaactaa gcaataaaaa ctaatgttcc cctcattctt tgaacttcta
     1561 ctatttgatt tatttattct gataaatgac gatttcacct tcaggatctt caaatcccgt
     1621 atagaattta tttaagatct cttcagtatc agtaatagga taggcgatag gccacatacc
     1681 ctttgatggt tagaataaac tgctcaaaat ctgtatcgta tgttggcttt agcatattaa
     1741 caaaggctat ctccaaatta taaaccataa gcgttgaatc gttcttaaca atttcaattc
     1801 ttcctaaccc aaagtcagaa gatataatta ccggacttgc cttacccttc atgtgggttg
     1861 ttgagattcc atatttaaag ggaattgtcg gtactatcgg cggtctgaaa aacatcccaa
     1921 aaataaaaaa atctttcccc tgctagtctc ctaagtccct ttgtagcgga tatggcacca
     1981 taatctcatt ttatcgaagt gagtcatggt ttatctgttg gccaaccaaa attgatacaa
     2041 tctaacgatg caccctctac ggcattctag gggagtgcac ttgcattcac ccaataaaaa
     2101 ccggcagtta gcaaagaact tatgtagtcg tatgttctta ctgttaacat acaagaggtc
     2161 tcctttccac aattgcttag cagtaaacgg gaatttacaa agctaatctc ttccattttc
     2221 ttatctttgt tctaaaagtc ccaaatggag caactgtatt tacatgaata aatttatata
     2281 cttcccatac agccgttttg gttgcctcgt ctgcccattt tcttttgtgt gcagtgaata
     2341 gatcttcatc actcattgaa tcaatcatca tgttaatatc atcaatatta cgattgagaa
     2401 tttcctctaa ttctttcaat ggtagatgtg cataggtatt tgtaaaccac ttatataact
     2461 ctcctaactg attccattta aattgatcag aaggcgtttt aacagataat ccatttcttt
     2521 catcattttc ccattttaga attaaattcg tccaaccaac ttgatatgct aaattttcag
     2581 caggtgttct atccacttcc tcaactcttt tatccttcaa ttcttcagga atagtttcaa
     2641 attctaaaat atatttagta taagtcctat ttatttcttt ttttaattct tctttatttt
     2701 tatatgttct cattatatta cctccataaa ttcctaattg tctcctttaa ttcatctata
     2761 atttcatcca cgatatttta atccgttcta caatgcatat ctgatatgca ttgactgtct
     2821 aactcatatg tgaatgattt ataaaatatc ccaacgtctc atctaaagaa atgttgatcg
     2881 tcagcaagga gctaaaaaat acgaatatat ttcttttgta gtaaaatcgg actggtttgc
     2941 atatcatttt cttcgtaaat atcaaaatca tgttaactac tatcaattac taataaaaac
     3001 taccacttta agcgttaaga actgttaagt ttagttaagt gaggatagtt cacaaaatcc
     3061 attgttaaac ttcacgaaag tggtaaaact caaaatgctt tatataggat tacagcatcg
     3121 ctctttcttc gattgcgcgt tgggtaaaaa atattatgaa gtaaaaattg acgatgacac
     3181 gttccttacg gctcatcaga tgaaacagct acaaaaacga aatgatcaac tcgaagaaaa
     3241 tttaattcta aaaaagcgat tgccatattc acgcctcgct cgacgaacta ttaaaggtca
     3301 agatttacct gaagatcatc ctttttagga aattgaagct ttttatgttg cacaatgcat
     3361 ttataatact acaatgatgt tttcaccaga tattcttcta gggtgcggtg taatgaaaca
     3421 gatacacttg caaaagaaaa ttcaccgcga atttactaaa attatgaata attatgttaa
     3481 tcttccgaaa tttttttcca aaattaaggg atcaagctgg tgttatagat tgtttaaggt
     3541 tggcaaaaaa ataacttctt tttaaacaat tatagaaaac cgatactctg ttaagttggt
     3601 tgacacggtg tcgattttta tgactttctt attctgaaac ttcactaacg gacagtacct
     3661 aaaaagtcta tagtatggac gtttacgagt actattttat caaaaatgct gctcatttca
     3721 acttatatgt tttagtgaac tacccccact tagcttggat aggctacaat taactagaca
     3781 gaaaaattaa ggtgtgtaga ctagaagaaa acataccagg aggaattttt atgtctaaga
     3841 gaacacgaag aactttttca caagaattca agcaacaaat cgtcaatctt tacttagctg
     3901 gaaagccacg tgtagaaatc attcgagaat atgaactaac
//
LOCUS       DACDKM010000161         3812 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20834_2016
            SAMN12640370-rid9891663.denovo.162, whole genome shotgun sequence.
ACCESSION   DACDKM010000161 DACDKM010000000
VERSION     DACDKM010000161.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640370
            Sequence Read Archive: SRR10040891
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3812)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3812)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742835.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742835.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:04:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,012
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 29 of 75
            Pseudo Genes (incomplete)         :: 33 of 75
            Pseudo Genes (internal stop)      :: 19 of 75
            Pseudo Genes (multiple problems)  :: 5 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3812
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640370-rid9891663.denovo.162"
                     /isolate="E20834_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            119..2179
                     /locus_tag="IV334_002676"
     CDS             119..2179
                     /locus_tag="IV334_002676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8944685.1"
                     /translation="MCSKRRSKQLNKHACRLDKERSCSEKHYVKFMMRTMLISLSVGV
                     YMHVPVQATTQTIKVVRTSVSETTDSPITITHTFTDANGKQLLPALTSYNKVSYPPTI
                     NHYWLIPNKTLWTSETGSSVPLSIFSDMTDPTKGDQLKQITDILNHALTDIKDGGSAQ
                     VSYVYEKDYSQFERKKIDVLAGTKVSISDLVERLKNADGTQGDMSKVISNNGNIVDTT
                     NTGTSTISLSYFDPIALKTMTASAVIHVRADQTAIKGKDLHLRTGESYAPKDLVEYVT
                     KSDGTSGNKEEIKMNGTVDTTTPGEYPVVLTYIDSKTSNVAETTAVVYVEASAASSTV
                     ITHFIDSQTNKELQAETHMSSVNRIPDLRLPAEFSDRVPNIDLSTYEQNGKVTSISDW
                     MQANGLDRKSHWSEILEVLHSELMNNRKQSGGEITFTYVYIPDNRSLEGHDLIVHPNE
                     KINKEELIQSATNSLGKPVPLKDVSVTINEVPLEDGYLTKQTGDFTIMYSYHDPYSLQ
                     ELHAQSLLHVLPLNKQIDQKEQKRREESLSEKKQQMAHAVSPTGKVKQTRSLELARIS
                     RKISESNKTIRQEAHLAPQVSGLENEILEPKIKQQSEKEFVAKQSEDQKESDDQKETK
                     KKRLSTIDSTDEHLQPQVPLPSPGTPAPGGGIPSSALGDFMRGMAGTWVYANRNEFDA
                     DDWF"
     gene            2262..2831
                     /locus_tag="IV334_002677"
     CDS             2262..2831
                     /locus_tag="IV334_002677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP8944686.1"
                     /translation="MLDKTLPYAEIWMTRPLQQNVPITPLAEGFEFRFYQKEDESAWA
                     EIETSVGEFQTTDEALEYFQQNFAPYPEELERRMFFVETKKGEKVATCTAWRKKSREQ
                     VYPVFHWLAVKPDYQGKGLAKALTARVLQEFPTLHTDGPIYLHTQTWSHQAIALYKKM
                     GFTFIAENLDGTPNPDYEKVMQVLSKLDK"
     gene            complement(3006..3812)
                     /locus_tag="IV334_002678"
     CDS             complement(3006..3812)
                     /locus_tag="IV334_002678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8944687.1"
                     /translation="MNHLKTFFTNSTKTAGRITNGIYILLRIIVVIMLVDQVWNQHWG
                     NTMLLLLTLILFEIPYFLEKILKIQIPNMLEIILLSFIFSSTVLGELSDFYGYFSMWD
                     TFLHAFSGFLAGGVGFSLVYLLNKNTKTMNLTPLLLAVVSFCFSMTVGVMWEFVEFSA
                     DKLLQFDMQKDTIIQQINSVELSKDGNTVYHVPDITRTIIESQNADGKTDKTIIQNGY
                     LDVGIMDTMKDLFVNFIGAVVFSVFGYLYSRNDYQKFTFIKNFIPKKIKR"
BASE COUNT         1357 a          685 c          696 g         1074 t
ORIGIN      
        1 aatcaaaaaa aatactctcc taatagtcca tcttcttttg attttattta tataatgaaa
       61 tcatcttatg aaaattacta cttcatgttt agacaagagt agggaggact aagaataaat
      121 gtgttcaaaa agacgttcaa aacaactaaa taaacacgct tgtcgtttag ataaggaacg
      181 gtcgtgttct gaaaaacact acgttaagtt tatgatgcgc acgatgctga tcagtttatc
      241 tgtaggtgtc tatatgcatg tacccgttca ggcaacgaca caaaccatca aggtcgtacg
      301 tacctctgtt tctgagacca ctgattcccc tatcactatt actcatactt tcactgatgc
      361 aaatggaaaa cagctacttc ctgcattgac ttcctataat aaagtcagtt atcctccaac
      421 gataaaccac tactggttaa ttcctaacaa aacattatgg acatcggaaa ccggaagcag
      481 tgtgccgctt tctatctttt ccgatatgac tgatcctaca aaaggtgatc agttaaaaca
      541 gatcactgat attttgaatc atgctttgac agatataaag gacggtggaa gcgcacaagt
      601 ttcttatgta tatgaaaaag attattcaca gtttgaaaga aagaaaatcg atgttctggc
      661 agggacaaaa gtttcgatat ctgatctggt agaaaggctg aagaacgcag atggaacaca
      721 aggagatatg tccaaagtaa tttcaaacaa tgggaatatc gtggatacga ctaatacagg
      781 aacatctact atttccttgt cctattttga tccaattgct ttaaaaacaa tgactgcatc
      841 tgctgtgatc catgttcgtg ctgatcaaac agctatcaaa ggaaaagatc ttcacttaag
      901 aacaggagag agttacgccc ctaaagatct tgttgaatat gtaacaaaaa gcgatggaac
      961 ttcaggaaat aaagaagaaa tcaaaatgaa cgggacagta gatactacaa ctccgggaga
     1021 atatcccgtt gtattaactt atatagactc gaaaacatca aatgtggcag aaacaacagc
     1081 tgtagtttat gtagaagcat ctgctgcttc atcgactgtc atcactcatt ttatagatag
     1141 tcaaacaaat aaagaacttc aagcagaaac acatatgagt agtgtaaatc gtattcctga
     1201 tttgcgccta cctgctgaat tttctgatcg agtgccaaat atagatttat cgacttatga
     1261 acaaaatggg aaagtcactt ctatttctga ttggatgcaa gcaaacggat tagacagaaa
     1321 aagtcactgg tcggagatcc ttgaagtact tcactcagaa ctgatgaata ataggaagca
     1381 atcaggaggg gaaattacct ttacttatgt ttatatacca gacaatcgtt cattagaagg
     1441 tcatgatctt attgttcatc caaacgaaaa aataaacaaa gaagagttga tccaatcagc
     1501 aacaaattcc ttaggtaaac ccgtaccgtt aaaggatgta tcggttacaa tcaatgaagt
     1561 accattggag gatggttatt tgaccaaaca aacaggtgat tttacgatta tgtatagtta
     1621 tcatgacccg tattcactcc aagaactcca tgctcagtca ctattacatg ttttaccatt
     1681 aaacaaacag atagatcaaa aagaacaaaa aagacgagaa gaaagcttgt cagaaaaaaa
     1741 acaacaaatg gctcatgcag tttctccaac agggaaagta aaacaaacaa gaagcctaga
     1801 gttagcaaga atatctagaa aaatctcaga atctaataaa acgataaggc aagaagctca
     1861 tttggcccca caagtttctg gattggaaaa tgaaatttta gagcctaaaa taaaacaaca
     1921 atcagaaaaa gaatttgttg ctaaacagtc ggaagatcag aaggaatcag atgaccagaa
     1981 ggaaacgaag aaaaaacgct tgtcaactat tgattccacc gatgagcatc tgcagccgca
     2041 agtaccactt cctagtccgg gaacacccgc acctggtgga ggtattcctt cttctgcttt
     2101 aggagatttc atgcgaggta tggcaggaac atgggtatac gctaatcgaa atgaatttga
     2161 cgcagatgac tggttctgat ttatatctct atctgttata gtcaaataag tagctgagtc
     2221 atatgttatt tggcataatg gagacgagga gggatagaaa aatgttagat aaaacgttac
     2281 cttacgcaga aatctggatg acaagaccac ttcaacaaaa tgttccaatc acgcctttag
     2341 cggaaggttt tgaatttcga ttttatcaaa aagaagatga atctgcttgg gcggagattg
     2401 agacttccgt tggggaattt caaacgacag atgaagcact tgagtatttt caacaaaact
     2461 ttgctcctta tcctgaagaa cttgaaagaa ggatgttttt tgtagagaca aaaaaaggag
     2521 aaaaagtagc aacctgtacc gcttggcgaa aaaaatcaag agagcaagtg tatccggttt
     2581 ttcattggtt agcagtgaaa ccagattacc aaggaaaagg gctcgcaaaa gcattgactg
     2641 ccagagtctt gcaagaattt cctaccctcc atacggacgg accaatctat cttcacacac
     2701 agacatggag ccatcaggcg attgctcttt ataaaaaaat gggttttact tttattgctg
     2761 aaaatctaga cggcacacct aatccagatt atgaaaaagt tatgcaggtt ttaagtaaat
     2821 tagataaata acgaaatggt gaaataagtc ctcagtattg ggataaaaaa cgaaatagtc
     2881 attcctcata tcttactttt ttgagttaat actatcggat aactaatggc atgccataca
     2941 ttcaaaaaag aaggggcagt gacaaaagta ttgtcactgc cccttctctt gttttttagc
     3001 atgttttatc gtttgatttt tttaggaata aaattcttga tgaacgtaaa tttttggtaa
     3061 tcgtttcgac tatagaggta accaaataca ctgaaaacaa ctgctccaat aaaattaaca
     3121 aataaatcct tcatagtatc catgattccc acgtctaagt aaccattttg gatgattgtt
     3181 ttatctgttt taccatctgc attttgactt tcaatgattg tccgagtgat atctggaaca
     3241 tgatagacag tgtttccgtc tttacttaac tccacactat tgatttgctg gatgatcgta
     3301 tctttttgca tatcgaattg cagtaattta tctgctgaaa attccacaaa ctcccacata
     3361 acaccaactg tcatcgaaaa acagaaggaa acaactgcta gtaataaagg agtcaagttc
     3421 atggtttttg tatttttatt gagcaaataa acaagggaaa agccgacacc cccagctaaa
     3481 aatccgctga atgcatgtag aaaagtatcc cacattgaaa aatagccata aaaatcactt
     3541 aactctccta aaactgttga actaaaaata aaagataaca aaatgatttc tagcatattt
     3601 ggaatctgta tctttaatat cttttccaga aaataaggga tttcaaataa aattaatgtc
     3661 agtaataaaa gcattgtatt tccccagtgt tggttccaga cctgatccac tagcataata
     3721 acgacgatga tacgtaataa aatatagatt ccgtttgtga ttctacctgc agtttttgta
     3781 ctgttcgtaa aaaaggtttt caaatgattc at
//
LOCUS       DACDKM010000162         3792 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20834_2016
            SAMN12640370-rid9891663.denovo.163, whole genome shotgun sequence.
ACCESSION   DACDKM010000162 DACDKM010000000
VERSION     DACDKM010000162.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640370
            Sequence Read Archive: SRR10040891
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3792)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3792)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742835.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742835.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:04:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,012
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 29 of 75
            Pseudo Genes (incomplete)         :: 33 of 75
            Pseudo Genes (internal stop)      :: 19 of 75
            Pseudo Genes (multiple problems)  :: 5 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3792
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640370-rid9891663.denovo.163"
                     /isolate="E20834_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            127..579
                     /locus_tag="IV334_002679"
     CDS             127..579
                     /locus_tag="IV334_002679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000751236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8944688.1"
                     /translation="MKKTILSIFSVFTILIGGYFLLNLDSVNSNAQIKDQRVAEHNNK
                     KMSLTKNIYSMSDEELYKEFGDLSGEQLEESIPGISDSDKIVVLSAGGQLMGDGKSEL
                     VSYKQNHRQFIDVNTDSNAISIKDLKELLNSKRNELKQIKETNSVELE"
     gene            593..1282
                     /locus_tag="IV334_002680"
     CDS             593..1282
                     /locus_tag="IV334_002680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000718009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8944689.1"
                     /translation="MKKIFGVSVGLLMMLTFGNAVYAAEKEVISDPEQYASRYFADFE
                     EEDKVLALPEGGFLKGEAKIVSMDNPDIIQGSYNSETDPASVSVTEAKKIVAEKFKND
                     IETTPIMPRGAFPPDVGTSGHLLVLAGGGSYKSQPFSGAGWRFGGMGVKPADGTGNFL
                     RWKSYIDDGRVGNVNQANATKSGSIQGMALYAREGFKYFAPNGPGWTMNIGIYYTYNP
                     VAGTYYVVQNI"
     gene            1407..3758
                     /locus_tag="IV334_002681"
     CDS             1407..3758
                     /locus_tag="IV334_002681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000344083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1906 domain-containing protein"
                     /protein_id="HAP8944690.1"
                     /translation="MDEMVLSTQKWLNKKYSNVTGFDKVPENGRTGWPTIYGLIEGLQ
                     VELGITNLVANFGPTTEKMYDNQVTPKWGKNLPKNIVFLIQGAFWCKGINPGGFDGVY
                     TPYLDTAVKELQTDAGFNGSAVTGVLDAKWAKALFDMSAFVLVPGGDSKIRKMQQWLN
                     VNYLEYTGIMPCDGIYQRNSNQALIFALQAELGYSPSEATGSYGNGTTSKTYPVSEGN
                     SGNYVRIIQYGLYVNGYFEDGTFDGIFTKYMGLEVLAFRKFMVLPEYTEIADVVVIKG
                     LLSSAGDIRRSADGADTSTQLTRSQIQTLVDNDIKIVGRYLTGTVGIGKDERNKYLTT
                     EELNNIFSKNLSVFPIYQDGGAALAYFTYDRGLSDGKKAIDAAKNLNIPLTTVIYFAV
                     DLDMLGEEILAYAGEYFKGVSAVMSYSGYQTGVYGTRNVCSQIINNGYASFAFVSDMS
                     TGYSGNLGFPMPRQWSFDQFVEFTIGSGNGAVGIDLIATSGRDSGFNELSNDTNNDNY
                     IAKYNQKVINQMVRAYQYLSQAGLDNPWNPHLTFYRYVNYSGLSWDIISSPVTEHDRK
                     IYDEYRNKLTNSEGLYNYFIDPNTGSIIGLPHLIVTLQSQMFLADTINDSVSDFAGWL
                     GDLMTCWGEVKKLGIQMEQGVFALVGTAAGGTFSLEDLYQDVDAVNVYTLAKRNSDST
                     ALSALKTYYCDKGAIDRFNQFVKNRFGSSDKIASVTNSLLENDETVDILGVGIISFFI
                     DKIVNNSLGTDFTYLDFIGDEGKVANGFSEKIIQFQENRSKLN"
BASE COUNT         1284 a          499 c          779 g         1230 t
ORIGIN      
        1 attcctcctg ctatcttttt ctattattct atcttatttg atagtagatt taaaactttg
       61 caacagaacc aattatctta tcgtagatat atattataaa tttaataaga aagcgaggtg
      121 agaatcgtga aaaaaacgat actaagtatt ttttcagtat ttacaatatt aattgggggc
      181 tattttcttt tgaatttaga ttctgttaat tcaaacgctc aaataaaaga tcaaagagta
      241 gctgaacata ataataaaaa aatgagtttg accaaaaata tctattctat gtcagatgaa
      301 gaattgtata aagaatttgg tgatttatcc ggggaacagt tagaagaaag tattccagga
      361 attagtgact cagataaaat tgttgtcttg tctgctggtg ggcaattaat gggagatggc
      421 aaatccgaat tagttagtta taaacaaaat cacagacagt ttattgatgt taatactgat
      481 tccaacgcta tttcgattaa ggatctaaaa gagcttttaa atagtaaaag aaatgaatta
      541 aagcagataa aagaaactaa ttcagtggaa ttagagtaaa ggaagattca ttatgaaaaa
      601 gatttttggt gtttctgttg ggctacttat gatgttaaca tttggaaatg cagtatatgc
      661 tgctgagaaa gaagttattt cagatccaga gcagtacgcc tcacgatact ttgctgattt
      721 tgaagaagaa gataaagtac ttgcattgcc agaaggtggt tttttaaaag gggaagctaa
      781 aattgtatct atggataatc cagatataat tcaaggttca tataattcag aaactgatcc
      841 tgcttcagtt tcagtgacag aagcaaaaaa aattgttgct gaaaaattca aaaatgatat
      901 tgaaactact ccaattatgc ctcgaggagc atttccaccc gatgttggta ctagcggaca
      961 ccttcttgta ctagcaggag gaggaagcta taaatcacag ccatttagtg gtgcgggatg
     1021 gcgttttgga ggtatgggtg taaaaccagc tgatggtaca ggcaactttt tacgttggaa
     1081 atcctacatt gacgatggaa gagtaggaaa tgtaaatcaa gccaacgcaa ctaaatctgg
     1141 ttctattcaa ggaatggcat tgtatgctcg tgaaggattc aaatactttg caccaaacgg
     1201 gcctggttgg acaatgaata taggtattta ttacacttat aatcctgttg caggtacgta
     1261 ttatgttgtt caaaatattt aataaaacag cacaaagaga tttctttgtg ctgttttatt
     1321 atttttagtt gttgacaaac actaaaatat aaaatataat caacacataa acgaacgtat
     1381 gttcggcaag aaagcagggg atttttatgg atgagatggt tttaagtact cagaaatggc
     1441 taaacaagaa atacagtaat gttacagggt ttgataaggt tccagaaaat gggcgaacag
     1501 ggtggcctac aatctatgga cttattgaag gactacaagt agaacttggt attactaatc
     1561 tagtagctaa ttttggacct accactgaaa aaatgtacga taatcaggtt actcctaaat
     1621 ggggcaaaaa tctacctaaa aatattgtat ttcttattca aggtgccttt tggtgtaagg
     1681 ggattaatcc aggtggcttc gatggggtat atactccata tttagatact gctgtaaaag
     1741 agttgcagac tgatgcaggg tttaatggaa gcgctgtaac tggagttttg gatgcgaaat
     1801 gggccaaagc gttatttgat atgagtgctt ttgttcttgt tcccggcgga gatagtaaaa
     1861 ttagaaaaat gcagcaatgg ttaaatgtaa attatttaga atacacggga ataatgcctt
     1921 gtgatggtat ttatcaaaga aattcaaatc aagcattaat ttttgcatta caagctgaac
     1981 taggatattc tcccagtgaa gcaacagggt cttatggtaa tgggacaact agtaaaactt
     2041 atcctgtgtc tgaaggtaac tcgggaaact atgtaagaat aattcaatat ggattatatg
     2101 ttaatggtta ttttgaagac ggtacattcg atggtatttt cactaaatat atgggtttag
     2161 aagtactggc attccgaaaa tttatggtat tgcctgaata tactgaaata gcagatgttg
     2221 ttgtaataaa aggactattg tctagcgctg gagatattag aagatctgca gatggcgcag
     2281 atacttctac gcaattaact agatctcaaa tccaaacatt ggttgataat gatataaaaa
     2341 tagttggtag atatcttact ggtactgttg gaattggaaa agatgaacga aataagtatc
     2401 taactacaga ggaattgaat aatatttttt ctaaaaattt atctgtgttt cctatctatc
     2461 aagatggagg tgcagcatta gcttatttta cttatgatcg tggcttatca gatggtaaga
     2521 aggctataga tgcagcaaaa aatctaaata taccattaac aacagttatt tattttgctg
     2581 ttgatctaga catgttaggg gaagaaattc ttgcatatgc tggagaatat tttaaggggg
     2641 tatctgctgt aatgtcatat agcgggtatc aaacgggtgt ttacggaaca agaaatgttt
     2701 gttctcaaat aatcaataac gggtatgctt cgtttgcatt tgtttcagat atgtcgactg
     2761 gttatagtgg gaatttaggt tttcctatgc ctagacaatg gtcttttgat caatttgttg
     2821 aatttaccat aggaagtggc aacggagctg ttggtatcga tttaatcgct acatcgggta
     2881 gagatagcgg atttaatgag ttatcaaatg atactaataa tgataattac attgccaaat
     2941 acaatcagaa ggttataaat cagatggtac gtgcgtatca atatctatca caagctggtc
     3001 ttgataatcc ttggaaccct catttgacat tttatagata tgtaaattat tctggtttgt
     3061 catgggacat catttcttct cctgtaacag agcatgatag aaaaatttat gatgaatata
     3121 ggaataaatt aactaatagc gagggcctgt ataattattt tatagatcct aatactggtt
     3181 caataatagg tttgcctcac ttaattgtga cgttacaatc acagatgttt ctagccgaca
     3241 caatcaatga tagtgtttct gactttgcag gttggttagg agatttaatg acttgctggg
     3301 gtgaagttaa gaaattagga atacaaatgg agcaaggtgt atttgcatta gtaggtacgg
     3361 ctgcaggtgg aacatttagt ctagaagatt tataccaaga tgttgacgcg gtaaatgttt
     3421 ataccttagc aaaaagaaat tctgattcaa cagctttatc ggctttaaaa acgtactatt
     3481 gtgataaggg tgctattgat agatttaatc aatttgttaa gaacagattt ggttcttcag
     3541 ataaaatcgc ttcagtaaca aatagtttat tagaaaatga cgaaacagtt gatatcttag
     3601 gtgtagggat aatttcattt ttcattgata aaatagttaa taattcactt gggacagatt
     3661 ttacttatct tgattttatt ggagatgagg ggaaagttgc aaatggattt tctgaaaaaa
     3721 ttattcaatt tcaagaaaat agaagtaaac ttaattaaga atcgtttact atttaaacag
     3781 tgtccttttc ct
//
LOCUS       DACDKM010000163         3713 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20834_2016
            SAMN12640370-rid9891663.denovo.164, whole genome shotgun sequence.
ACCESSION   DACDKM010000163 DACDKM010000000
VERSION     DACDKM010000163.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640370
            Sequence Read Archive: SRR10040891
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3713)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3713)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742835.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742835.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:04:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,012
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 29 of 75
            Pseudo Genes (incomplete)         :: 33 of 75
            Pseudo Genes (internal stop)      :: 19 of 75
            Pseudo Genes (multiple problems)  :: 5 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3713
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640370-rid9891663.denovo.164"
                     /isolate="E20834_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(162..2159)
                     /gene="tkt"
                     /locus_tag="IV334_002682"
     CDS             complement(162..2159)
                     /gene="tkt"
                     /locus_tag="IV334_002682"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HAP8944691.1"
                     /translation="MFDKIDQLGVNTIRTLSIEAVQKANSGHPGLPMGAAPMAYALWT
                     KHLKVNPTTSKNWADRDRFVLSAGHGSAMLYSLLHLAGYQVTIDDLKQFRQWDSKTPG
                     HPEVHHTDGVEATTGPLGQGIAMAVGMAMAEAHLAATYNKENFNVMDHYTYAICGDGD
                     LMEGVSQEASSMAGHMKLGKLIVLYDSNDISLDGPTSKAFTENVGARYEAYGWQHILV
                     KDGNDLEAISKAIEEAKAETDKPTLIEVKTVIGYGAPKEGTSAVHGAPLGEDGIKMAK
                     EVYGWEYPDFTVPEEVAARFHQTMIEEGQKAEDAWNEMFANYKKAYPELAQQFEDAFD
                     GKLPENWDAELPTYEVGSSQASRVSSKEVIQELSKAIPSFWGGSADLSGSNNTMVAAD
                     KDFTPEHYEGRNIWFGVREFAMASAMNGIQLHGGTRIYGGTFFVFVDYLRPAVRLAAI
                     QHTPVVYVLTHDSVAVGEDGPTHEPIEQLASVRCMPGVQVIRPADGNETRAAWKVAME
                     TTDAPTILVLSRQNLPVLPSTKEVADEMVKKGAYVLSPSQGETPEGILIATGSEVDLA
                     VKAQKELAEKGKDVSVVSMPSFDLFEKQSSEYKESVLPKSVKKRVAIEAAASFGWERY
                     VGTEGATITIDHFGASAPGTKILEEFGFTVENVVNTYNQLS"
     gene            complement(2261..2512)
                     /locus_tag="IV334_002683"
     CDS             complement(2261..2512)
                     /locus_tag="IV334_002683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF896 family protein"
                     /protein_id="HAP8944692.1"
                     /translation="MLSPEKIERINELARKKKENGLSKAEEQEQINLRKEYLEAFRSG
                     MRHHIEGMKVVDPEGNDVTPEKLKEIQKKKGLHNRNDNF"
     gene            2659..3282
                     /gene="lexA"
                     /locus_tag="IV334_002684"
     CDS             2659..3282
                     /gene="lexA"
                     /locus_tag="IV334_002684"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HAP8944693.1"
                     /translation="MARQTESRQIEVLRYIHEQVTQKGYPPTVREIGEAVQLSSTSTV
                     HGHLARLEKKGFIQRDPTKPRAIELTKQGLEKIGIRPTTIPVLGVVTAGEPILAVEDA
                     SDFFPIPPNLSSEEGSLFMLTIRGDSMINAGILDGDNVIVRKQETAQNGDIVIAMTAE
                     DEATCKRFYKEKNFFRLQPENDLLEPIILDQVSILGRVVGLYRSHIY"
     gene            3462..3680
                     /locus_tag="IV334_002685"
     CDS             3462..3680
                     /locus_tag="IV334_002685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8944694.1"
                     /translation="MKSMRRLGLVLTVLFLGIIIGNFGIRPALLVFVPLFTLWFMLWD
                     EKKYRQSERRRIHEDHFYHEPYYRSRQS"
BASE COUNT         1025 a          797 c          688 g         1203 t
ORIGIN      
        1 ctcaagaaac acaaactaat ccgaagaatt ctttgttttg tcatttaaat gtaacatagc
       61 cgaaaacgaa aagtaatatt gatttattct tctaacacat aaagaacagc tgtctccaac
      121 ttgcagacag ctgttcttat cagtcaaaca attgcttgga tctatgatag ttgattgtac
      181 gtattcacga cattttctac tgtaaatcca aattcttcta aaattttagt gccaggggca
      241 gatgcaccga aatgatcgat tgtaatcgtt gcgccctctg ttcctacgta gcgttcccat
      301 ccaaatgatg cagcagcttc gatagccaca cgtttcttca ctgatttagg aagtacactt
      361 tctttatatt cagaagattg tttttcaaag aggtcaaagc ttggcataga aacaacagaa
      421 acatctttgc ctttttcagc caattctttt tgagctttca cagccaaatc aacttcagaa
      481 cctgttgcaa tcaaaatacc ttctggtgtt tctccttgtg atggagaaag aacatacgcg
      541 ccttttttca ccatttcatc tgctacttct tttgtagaag gcaatactgg aaggttttga
      601 cgactcaata caaggatagt aggcgcatct gttgtctcca tcgctacttt ccaagcagca
      661 cgtgtttcat ttccgtctgc cggacgaatc acttgaacac caggcatgca gcgaacagat
      721 gctagttgtt caatcggttc gtgcgttggt ccgtcttctc cgacagcaac cgagtcatgt
      781 gttaagacgt aaacaacagg agtatgttgg attgctgcta aacgaactgc tggtcttaga
      841 taatcgacaa agacaaagaa tgttccaccg tagatacgag ttccgccatg aagttggata
      901 ccattcatag ccgaagccat tgcaaattcg cggacaccga accagatgtt gcgtccttca
      961 taatgttcag gagtgaaatc tttatctgca gccaccattg tattattgga acctgacaag
     1021 tccgctgaac caccccagaa gcttggtata gctttcgata attcttggat gacttcttta
     1081 cttgaaacac ggcttgcttg actgcttcca acttcataag ttggcaattc tgcatcccag
     1141 ttttctggta gtttgccatc aaaagcatct tcaaattgtt gtgctaattc aggataagct
     1201 tttttataat tggcaaacat ttcattccat gcatcttcag ctttttgtcc ttcttctatc
     1261 atcgtttgat ggaagcgggc agctacttct tctggaacag tgaaatctgg atactcccaa
     1321 ccataaactt ctttagccat cttgatgcca tcttcaccaa gaggagcacc atgaacagca
     1381 gatgttcctt ctttaggagc accatagccg atgactgttt ttacttcgat caatgttggt
     1441 ttgtctgttt cagcttttgc ttcttcaatc gctttagaga ttgcttctag gtcatttccg
     1501 tctttcacta agatatgttg ccagccatat gcttcatagc gagcaccaac gttttctgtg
     1561 aaagcttttg aagttggacc gtctagtgaa atatcgtttg aatcgtataa aacgatcaat
     1621 ttccctagtt tcatatgacc tgccatcgaa cttgcttctt gagatacccc ttccatcaag
     1681 tctccgtcac cgcatattgc atacgtatag tgatccatca cattaaagtt ttctttgtta
     1741 tacgttgctg caaggtgcgc ttctgccatt gccataccga cagccattgc aatcccttgt
     1801 cctaatggcc ctgtagtggc ttctacacca tctgtatgat gtacttcagg atgtcctggt
     1861 gttttagaat cccattgacg gaattgtttc aaatcatcga ttgtcacttg atagccagct
     1921 aaatgaagca agctatacaa cattgctgac ccatgtcctg ccgaaagaac gaaacggtcg
     1981 cggtcagccc agttttttga tgtcgttgga tttactttca ggtgttttgt ccataacgca
     2041 tatgccatag gtgctgcgcc catcggcaat ccaggatgtc ctgagtttgc tttttggact
     2101 gcttcaatac ttaatgtacg aattgtgtta acgccaagtt ggtcaatttt gtcaaacaaa
     2161 ataacccctc ctattaattt aggttttttt tacctttatt tcttacactt tcattctata
     2221 agatgtcaca cgaaaaagca atctttttca gtatttttta ctaaaagttg tcatttcggt
     2281 tgtgtaagcc ttttttcttt tggatttctt ttaatttttc cggggtcacg tcattgccct
     2341 caggatcgac gactttcatt ccttcgatat gatgacgcat accggaacga aaagcttcca
     2401 aatactcttt gcgcaagttg atttgttctt gttcttcggc ttttgagaga ccattttcct
     2461 tctttttacg tgcaagttca ttgatccgct ctattttttc aggtgacagc atgctcagcc
     2521 ctccttttac tctccttcat attatataaa aactgaaaaa aaaacaacta gataaactaa
     2581 ataaaatacg aacattcgtt tgatttagcg ctttttatat ggtactattt atttatcaag
     2641 tgaaagaagg tgtaccttgt ggcccgacaa actgaatcaa gacaaatcga agtcttacga
     2701 tatatccatg aacaagtaac ccaaaaagga tacccgccga ctgtccgtga aatcggggaa
     2761 gctgttcagc tttcttctac ttctactgtt cacggtcatc ttgcacggct tgaaaaaaaa
     2821 gggtttatcc aaagagatcc gacgaaacct cgtgcaatcg agttgacaaa acaaggttta
     2881 gaaaagattg ggattcgtcc aacaactatt ccagtacttg gcgtcgtaac agctggagaa
     2941 cccattttag cagtagagga tgcttcagac ttctttccaa tccctcctaa tctttcttcc
     3001 gaagaaggca gtctctttat gctgacaatt cgtggagata gtatgatcaa tgctgggata
     3061 ttggatggtg acaacgtgat cgttcggaag caagaaactg ctcaaaatgg cgatattgtc
     3121 attgcaatga ctgccgagga cgaagcgact tgcaaacgat tctataaaga aaaaaacttt
     3181 ttccgtctgc agcctgaaaa cgacttgctc gaaccaatca tcttagatca agtatctata
     3241 ttaggacgag tagtaggact ttatcgtagt catatctatt aattgctact aattaaaaga
     3301 aaacttttta aaaagagttt gagacgttgg tctcaaactc ttttttcgcc cgggttcttc
     3361 ttctttcaaa tttttatcac ttccctcttt acaaaccgaa caaatattcg tatactaaat
     3421 atacgaacaa ttgttcgcat aaattaaaga ggtgagaatt catgaaatca atgagacgtc
     3481 ttggcttagt attaactgtt ttgtttttag gtattattat tggtaatttc ggcatacgtc
     3541 cagctttact tgtgtttgtt ccgttattta ctttgtggtt catgttgtgg gatgaaaaga
     3601 agtaccgtca atctgagcgg cgcagaattc atgaagatca tttctatcat gagccctact
     3661 acagatccag gcaatcataa taaggattga ctgctaatta ttcaaaagac aag
//
LOCUS       DACDKM010000164         3590 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20834_2016
            SAMN12640370-rid9891663.denovo.165, whole genome shotgun sequence.
ACCESSION   DACDKM010000164 DACDKM010000000
VERSION     DACDKM010000164.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640370
            Sequence Read Archive: SRR10040891
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3590)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3590)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742835.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742835.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:04:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,012
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 29 of 75
            Pseudo Genes (incomplete)         :: 33 of 75
            Pseudo Genes (internal stop)      :: 19 of 75
            Pseudo Genes (multiple problems)  :: 5 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3590
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640370-rid9891663.denovo.165"
                     /isolate="E20834_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(<1..1015)
                     /gene="uxuA"
                     /locus_tag="IV334_002686"
     CDS             complement(<1..1015)
                     /gene="uxuA"
                     /locus_tag="IV334_002686"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015694718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HAP8944695.1"
                     /translation="MEMTFRWYGANEEKISLQEIRQIPAMKGIVGTLMDIPAGEVWPR
                     ERIKALKDEVEANGLTLKVIESVNVHDDIKIGLPTRDQYIENYKQTIRNLAEFGIEVI
                     CYNFMPVFDWVKSDLDYHLDDGSSTLAYISKNIPTDPEEIIRKVENSTNNFELPGWEP
                     HRLSQIKQLLEAYKEVDEAKLRENFAYFLKAIIPTCEEVGIKMAVHPDDPPYSIFGLP
                     RIVKNRDDLEWLCNVVDSPSNGITLCTGSIAERPDNDVYEILSEFCQRDRIPFAHVRN
                     IKYLSDNGLDFYEAPHKSEYGSLDMYKILKTMYDNGFNGYIRPDHGRMIWGETGRPGY
                     GLYD"
     gene            complement(1018..2649)
                     /locus_tag="IV334_002687"
     CDS             complement(1018..2649)
                     /locus_tag="IV334_002687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HAP8944696.1"
                     /translation="MVLIKDWQDSIEGLNKMQVQLPYFNVEAVKQEGQKHPVWLHFGG
                     GNLYRGFHAEIAQCLADQNELESGVVVCETFDEEVVEKAYHDYNNDILEIVMGEDGEL
                     KKRLLAVTADSLYCNPKNITDYQRVVKYFEDPTLQFTTFTITEKGYSLKDSKGQFFSV
                     VEADIKSGPTNPQHTMSIVTSLLYSRFNAGELPIAMVSTDNFSQNGQRFQDSIFMIAE
                     AWQKKGYVSEKFIAYLKDKEKVTFPWSMIDRITPNPSESVKMDLLKAGFEDLDIIHTA
                     KNTNIAAFTNTEKVHYLVIEDSFPNGRPKLEAAGVIMTDRETVDKADIMKVTTCLNPL
                     HTALAITGCLLGYKSIQAEMQDDDLVGLIKGIGYVEGLPVVESPGVIDPKQFIDEVIN
                     KRLPNPYIPDTPQRIATDTSQKVAIRYGETIKKYVEKDGNAENLTFIPIAIAAWLRYL
                     LAVDDQGKNFVPSPDPLLVELQEKFSVLKLGEQDPAKIHCAVQEILENKEIFGLDLYQ
                     VEIGKKIEKIFCEMLVGPQAVRKTINKYITEVGGK"
     gene            2966..>3590
                     /locus_tag="IV334_002688"
     CDS             2966..>3590
                     /locus_tag="IV334_002688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAP8944697.1"
                     /translation="MTIKTSLQNQAYQSIRQQIIYADLEPGKKVSEKKLEETLSIGRT
                     PIREALIQLRHQGLVDTIPQSGNYISLIDLDLAKNARFVREHLERQIMLECCAKMNNQ
                     KKQVLETIIAEQEKAVSQKNARSFFHNDNLFHKACFEIAEREEVWQWLEDNNTHLERF
                     RWLRVKTEGLKWEKIMEQHHQLFKALTMQDPEEANFLTSLHLHLMLDE"
BASE COUNT         1153 a          694 c          519 g         1224 t
ORIGIN      
        1 ggtcatataa cccataacca ggtctaccgg tttctcccca aatcatccga ccatgatctg
       61 gacgaatata gccattaaat ccattatcgt acattgtttt taatatctta tacatatcta
      121 gagaaccata ttctgattta tgcggcgctt cataaaaatc gagtccatta tcacttaaat
      181 atttgatatt acgaacatgt gcaaaaggaa tccgatcccg ttgacaaaat tcagatagaa
      241 tttcatagac atcattatct ggtcgttctg caatacttcc agtacataac gtaataccat
      301 tagaaggaga atccactaca ttacacaacc attctaaatc gtcacgattt ttaacaatac
      361 gaggtaatcc gaaaatagaa tatggtggat catctggatg aactgccatt ttaatcccaa
      421 cttcttcaca tgtcgggata attgctttga gaaaatatgc aaagttctct cgcaatttcg
      481 cttcatcaac ttctttatag gcttctaata attgcttgat ttgtgataac cgatgaggtt
      541 cccaacccgg caattcaaaa ttattagtag aattttctac ttttctaata atttcctctg
      601 gatcagtagg aatatttttt gaaatatagg ctaacgtaga agacccatca tctaaatgat
      661 aatccaaatc tgattttacc caatcaaaga ctggcataaa attataacag ataacctcaa
      721 tcccaaattc tgccaaattg cgaatcgtct gtttataatt ctcgatgtat tgatctcgtg
      781 ttggcaaacc aattttgata tcatcatgaa cattaacact ctcaataact ttcaaggtaa
      841 ggccattggc ttcgacttca tcttttaaag ctttaattcg ttctctcggc cagacctctc
      901 cggctggtat atccattaat gttccaacaa ttcctttcat tgctgggatc tgacgaattt
      961 cttgtagtga gattttttct tcatttgccc cataccatct aaaagtcatt tccatagtta
     1021 ctttcctcca acttctgtta tatatttatt gattgtttta cgaactgctt gaggacctac
     1081 tagcatttcg caaaagattt tttcaatttt ctttcctatc tcaacttgat ataaatctaa
     1141 accaaatatt tccttatttt ctaagatttc ctgtaccgca caatgaatct ttgcaggatc
     1201 ttgttcacct aatttcaata ctgagaattt ttcctgcaac tctaccaata aaggatcagg
     1261 acttggcaca aaattttttc cttgatcatc gactgccaat aaataacgta accaagcagc
     1321 aatagcgata ggaataaatg ttagattttc tgcatttccg tctttttcga catacttttt
     1381 aattgtttca ccatagcgaa tcgctacttt ttgagaagta tctgttgcaa ttcgttgcgg
     1441 tgtatctgga atatagggat taggcaaacg tttattgatt acttcatcaa taaattgttt
     1501 tggatcaata acgcctggac tttcaactac tggtaagcct tctacataac ctatcccttt
     1561 aattaagcca accaaatcat catcttgcat ttctgcctga attgatttat accccaacaa
     1621 acaacctgtt attgctaatg ccgtatggag cggatttaaa catgtcgtaa ccttcatgat
     1681 atctgcttta tcaactgttt ctctatctgt cataatcaca ccagccgctt ccagtttagg
     1741 tcgaccattt ggaaaactat cttcaatgac tagataatgg actttttcag tattggtaaa
     1801 agcagcgata ttggtatttt tcgcagtatg aattatatct aagtcttcga aacccgcctt
     1861 caataaatcc attttgacac tttccgaagg atttggcgta attctatcaa tcatactcca
     1921 aggaaaagtc actttttctt tatcctttaa ataagcaata aatttttcag aaacgtatcc
     1981 ttttttttgc caagcttctg caatcataaa aatgctatct tgaaaacgtt gaccattctg
     2041 tgagaaatta tcagtagaaa ccatcgcaat cggtaactct ccagcattaa acctgctata
     2101 caacaaggat gtcacaatac tcatcgtatg ttgaggattg gtaggtccag attttatatc
     2161 tgcttccact accgaaaaaa attgtccttt agaatctttt aaactgtatc ctttttcagt
     2221 aatagtaaac gttgtaaact gtaatgtagg atcttcaaaa tatttaacca ctctttgata
     2281 gtccgttata ttctttggat tgcaatataa actatcagct gttactgcta aaagtctttt
     2341 tttgagttca ccatcttcac ccataacaat ctccaaaata tcattgttgt agtcatgata
     2401 agctttttct actacttctt catcaaatgt ctcacacaca actactccgg attctaattc
     2461 attttgatct gctaagcatt gagcaatttc tgcatgaaat cctcgataaa gatttcctcc
     2521 accaaaatga agccacactg gatgtttctg accttcttgt ttgaccgctt cgacattaaa
     2581 atacggtagt tgaacttgca ttttgttcaa tccttcgata gaatcttgcc agtctttaat
     2641 caaaaccata tttacccctc catactgata tgttagtttc cgttacaatt gtagtataaa
     2701 atcgaatgta atcgctgtca ataagaaaaa actaaaaata ttcttttttc taccatgtca
     2761 gaattattgt gtcttttttt agtaaaagca caccaatata tattttttag aactaactta
     2821 aattaaagct taatttctct ttgatcttat ttgagtattt tttgtttttt taatagctag
     2881 tatgttactt tgaaatttga tattatataa cgagcataga actaaaatat caaattcaaa
     2941 aatgaaattt tgagagggat taaatatgac aattaaaact agtctccaaa atcaagctta
     3001 tcaatctatt cgtcagcaaa ttatctacgc tgacttagaa cctggaaaaa aagtatcaga
     3061 aaaaaaattg gaagaaacgc tttctatcgg tcgtactcca atacgtgaag cattgattca
     3121 actccgtcat caaggacttg ttgatacaat tcctcaaagt ggaaattata tctcattaat
     3181 cgacttagat ttagcaaaaa atgctcgttt tgtcagagaa catttagaac gacaaattat
     3241 gctagagtgt tgtgccaaga tgaataatca aaagaaacaa gttttagaaa ctataattgc
     3301 agaacaagaa aaagctgtta gtcaaaagaa tgctcgtagt ttttttcata atgataattt
     3361 atttcataaa gcttgttttg aaattgcaga acgggaagaa gtatggcaat ggttggaaga
     3421 taataatacg cacttggaac gatttcgttg gttacgagta aaaacggaag gattaaaatg
     3481 ggaaaaaatt atggaacaac atcatcaact atttaaagcc ttgaccatgc aagaccctga
     3541 agaagctaac ttcttaacat ccttgcatct tcatttaatg cttgatgaac
//
LOCUS       DACDKM010000165         3385 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20834_2016
            SAMN12640370-rid9891663.denovo.166, whole genome shotgun sequence.
ACCESSION   DACDKM010000165 DACDKM010000000
VERSION     DACDKM010000165.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640370
            Sequence Read Archive: SRR10040891
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3385)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3385)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742835.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742835.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:04:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,012
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 29 of 75
            Pseudo Genes (incomplete)         :: 33 of 75
            Pseudo Genes (internal stop)      :: 19 of 75
            Pseudo Genes (multiple problems)  :: 5 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3385
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640370-rid9891663.denovo.166"
                     /isolate="E20834_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            89..1510
                     /gene="gndA"
                     /locus_tag="IV334_002689"
     CDS             89..1510
                     /gene="gndA"
                     /locus_tag="IV334_002689"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent phosphogluconate dehydrogenase"
                     /protein_id="HAP8944698.1"
                     /translation="MSKQQIGVVGMAVMGKNLALNIESRGYSVALFNRTGAKTTAVVE
                     EHPDKNFKATYTIEEFVESIEKPRRILLMVKAGPATDATIQELLPHLDKGDILIDGGN
                     TFFKDTMRRNEELANSGINFIGTGVSGGEEGALKGPSIMPGGQKEAYALVAPILEQIS
                     AKAEDGAPCVTYIGPNGAGHYVKMVHNGIEYGDMQLIAESYDLMKNILNLSVEEMADI
                     FKEWNQGELDSYLIEITADILTRKDDEGTGKPVVDVILDAAGNKGTGKWTSQSALDLG
                     VPLPLITESVFARYISAYKEERVQASKILSRTNDFEFTGDKKELVEKIREALYFSKIM
                     SYAQGFAQLRVASKEFDWDLPFGEIAKIWRAGCIIRARFLQKITDAYDKNPEIENLLL
                     DDYFVEITKKYQQSVRDVVALAVQAGVPVPTFSSAIAYFDSYRAERLPANIIQAQRDY
                     FGAHTYERVDKEGIFHYSWYHEE"
     gene            1703..2389
                     /locus_tag="IV334_002690"
     CDS             1703..2389
                     /locus_tag="IV334_002690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010763438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP8944699.1"
                     /translation="MSNILIIEDEKNLARFVELELKHEGYNAEVHYNGRTGLDAALNN
                     EWDAILLDLMLPELNGLEVCRRVRQVKNTPIIMMTARDSVIDRVSGLDHGADDYIVKP
                     FAIEELLARLRALLRRIDIEGDKNVAKQTTITYRDLTIEKENRVVRRGNEVIELTKRE
                     YELLLTLMENVNVVLARDVLLNKVWGYETEVETNVVDVYIRYLRNKIDVPGEESYIQT
                     VRGTGYVMRS"
     gene            2386..>3385
                     /locus_tag="IV334_002691"
     CDS             2386..>3385
                     /locus_tag="IV334_002691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing protein"
                     /protein_id="HAP8944700.1"
                     /translation="MIKPKKSEKRELKGPSLTIKWAFASSFFIFVVFTIFAVITYKSS
                     INLIVAKERNNVERTISEVASRLANSDEELTLVNTYRNLTKATNDGDEVVDHTSLVEG
                     NLMKIDSFIAELGQPSMDLYVYDLDENLIFKTHENSRNLIQTTRKVPTIVTLEGKTGF
                     LSIQPIYSKGTREKIGYAQSFYELSSFYDIRNKLLLTLIVLEVVSLILSSILGFFLSS
                     YFLKPLKVLRDTMDTIRKDPQSDIHMPEIDTNDELADLAEIFNEMLDRMRLYIEQQEQ
                     FVEDVSHELRTPVAIIEGHLSLLNRWGKDDPEILEESLSASMQEISRMKSLVQEMLD"
BASE COUNT         1129 a          594 c          734 g          928 t
ORIGIN      
        1 aatatgttgt tctttagttt gcaatgtagc cgtgttaagc tataatgtaa gagattaggg
       61 agaaatcccg tatgtaggag gagttcatat gtcaaaacaa caaatcggcg ttgtcggtat
      121 ggccgttatg ggtaaaaatc tagccttgaa catagaaagc cgcggctatt cagtggcttt
      181 attcaatcgt acaggggcta aaacaaccgc tgtagtagaa gaacacccag ataaaaattt
      241 caaagcaaca tatacaatcg aagaatttgt agaatctatc gagaaaccac gtcggatctt
      301 gctaatggtc aaagcaggac cggcaacaga tgcaacaatc caagagttac ttcctcacct
      361 tgataaaggc gatattttga ttgacggagg aaatacattc tttaaagata cgatgcgtcg
      421 taatgaagag ttagccaact ctggcatcaa ctttatcggt acaggcgttt ccggtggtga
      481 agaaggagct ttaaaaggac cttcaatcat gcctggtgga caaaaagaag cctatgcgct
      541 agttgcacca atcttagagc aaatttctgc aaaagcagaa gacggagcac catgtgtcac
      601 atatattggc ccaaatggag ccggacatta tgtgaaaatg gtacacaacg gtattgagta
      661 tggagatatg caactgattg ctgaatctta tgacttaatg aagaatattt taaacctttc
      721 agtagaagaa atggctgata tctttaaaga atggaatcaa ggcgaattag acagctattt
      781 gatagaaatc actgctgaca ttctgacacg taaagacgat gaaggaacag gtaaaccagt
      841 agtagatgtg atccttgatg cagctgggaa taaaggaaca ggtaaatgga caagccaaag
      901 cgcgcttgac ttaggtgtgc cacttccatt gatcactgaa tctgtatttg cccgctatat
      961 ttctgcttat aaagaagaac gcgtacaagc aagcaagatt ttgtctcgta cgaacgattt
     1021 tgagttcacg ggagacaaaa aagaattggt cgaaaaaatc cgagaagcgt tgtatttcag
     1081 taaaatcatg agttatgccc aaggatttgc tcaattacgt gtcgcttcaa aagaatttga
     1141 ttgggacttg ccatttggtg aaatcgcaaa aatctggcgc gctggatgta tcatccgagc
     1201 tcgattcttg caaaaaatta cggatgccta tgacaaaaat cctgagatcg aaaatttatt
     1261 gctagatgat tacttcgttg aaatcacgaa aaaatatcag caatcagtca gagacgtcgt
     1321 tgccttggct gtccaagctg gcgtacctgt accgactttc tcatcagcca tcgcttactt
     1381 cgattcatac agagcagaac gtctgcctgc aaatataatc caagcccaac gcgattactt
     1441 tggcgcacat acctatgagc gtgtcgacaa agaaggaatc ttccattact catggtacca
     1501 tgaagaataa tagtctttcg gctgttagga aatgagaaaa taaaaagagg acgaaagagc
     1561 tgaagtcgat tgttatgagt agacttcagc tcttttttaa aattgctatc catgataaaa
     1621 tgactataga tttttaagca tattatcggt ataatgatag agtttttaaa aagccagtag
     1681 aatagaaagg ataaaataat aaatgagcaa tattctaatc attgaagatg aaaagaactt
     1741 ggcacgattt gtcgagctag agttgaagca tgaagggtat aacgcagaag tccactacaa
     1801 cggccgaact ggtttagatg cggcattaaa taatgagtgg gatgccatat tgttggatct
     1861 gatgctccca gaattgaacg gtttagaagt ctgcagacgt gtacgccaag tcaaaaatac
     1921 accaatcatt atgatgactg caagagattc tgtgatcgat cgtgtatctg gacttgacca
     1981 cggagcagat gactacatcg tcaaaccatt tgctatcgaa gaattattag cacgtcttcg
     2041 tgcattgttg cgtcgaatcg atattgaagg cgataaaaat gtggctaaac aaactactat
     2101 tacttatcgc gatttgacta tcgaaaaaga aaaccgtgtc gtacgtcgcg gaaatgaagt
     2161 aattgaacta acaaaaagag aatacgagtt gctgcttact ttaatggaaa acgtgaacgt
     2221 ggtattagca cgtgacgttt tactaaataa agtgtgggga tacgaaacag aagttgaaac
     2281 aaacgtagtg gacgtatata ttcgttatct tcgcaacaaa attgacgttc ccggggagga
     2341 aagctatatc caaactgtac gcggaacagg ttatgtgatg cgctcgtgat aaagccgaaa
     2401 aaatcggaaa aaagagaact gaaagggccc tccttgacaa tcaagtgggc ctttgcgagt
     2461 tctttcttca tattcgttgt gtttacaatt ttcgctgtca ttacctataa atcatctatc
     2521 aatctgattg tggcaaaaga aagaaacaat gtagagcgta cgatttcaga agtagcttct
     2581 cgtttggcta actcggatga agaactgaca ttagtaaata cgtatcgtaa tctgacgaaa
     2641 gcgacaaatg acggagatga ggtcgtagat catacttcac tggtcgaagg aaacttaatg
     2701 aagatcgatt cctttatagc tgagctaggg cagccatcaa tggatcttta tgtttatgat
     2761 ttggacgaga acttgatttt taaaacccat gaaaacagtc gaaacctgat tcagactaca
     2821 cgtaaagttc caacgatcgt tacactagaa ggaaaaactg gctttttatc tatccagcct
     2881 atatattcta aaggtacaag agaaaagatc ggctatgcgc aaagcttcta tgaattatct
     2941 tctttctatg atattcgaaa taagctatta ttgactctta ttgttttaga agttgtttct
     3001 ttgatcttaa gtagtatttt aggtttcttt ttatcttctt atttcttaaa accactcaag
     3061 gtactcagag atacaatgga tactatacgt aaagatccgc aatcagatat ccacatgcct
     3121 gaaatcgaca caaatgatga attggcagat ttagctgaaa tattcaatga gatgcttgac
     3181 cgtatgcgtt tgtatattga acaacaagag caattcgtgg aagacgtatc tcatgagtta
     3241 aggacacctg ttgcaattat tgaagggcat cttagtctgc taaatcgttg ggggaaagat
     3301 gatcctgaga tattagaaga atcattaagc gccagcatgc aagaaatcag ccgtatgaaa
     3361 agcttagttc aagagatgct agact
//
LOCUS       DACDKM010000166         3275 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20834_2016
            SAMN12640370-rid9891663.denovo.168, whole genome shotgun sequence.
ACCESSION   DACDKM010000166 DACDKM010000000
VERSION     DACDKM010000166.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640370
            Sequence Read Archive: SRR10040891
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3275)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3275)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742835.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742835.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:04:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,012
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 29 of 75
            Pseudo Genes (incomplete)         :: 33 of 75
            Pseudo Genes (internal stop)      :: 19 of 75
            Pseudo Genes (multiple problems)  :: 5 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3275
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640370-rid9891663.denovo.168"
                     /isolate="E20834_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(<1..2303)
                     /locus_tag="IV334_002692"
     CDS             complement(<1..2303)
                     /locus_tag="IV334_002692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8944701.1"
                     /translation="MRNKFHKLLSMFLVMLIVINQFGNITVLAESLAEQPERSLTIEV
                     DQEEAALGEQFQLRVRGTPDLLNALVIDKSEDLIEGEVKQLNDYERLITYSSQHSGKY
                     ELTARSGDIQSNTVSIEISVPEESTSESFNVPEESTSESFNIPEESLSESFNEARSSE
                     PSITQESQIERQEELLNYDIGISAERPTLPSRHTQTLKGYIQGGAGDILEEYGEMVVR
                     VPRASIGLIEFIRVEVADDSPLKPLAFYQEGTDVVFPFTFDYEGLNEAAQSYTFSITF
                     EVAFFQSGGTEPNNDFQVSLTYAERAANTEFSITPIPSSRPIFSKLYYGSREIDSQGN
                     LGNLLIDGQDVTNSNKFILQVNYEGSRKDGVVVTDVLPQGTTLAPNPTTWSSTGNREP
                     ISGGVRIAEVTNPGEANVRTHYVSEEFVDKVSFNKETNTLVVDFGNLAEGQSYFIEYA
                     LEVTDVDLFNEGRQEDGFSRNRASMTYNNQVMSRDYRLRIPESNLPAISLRKSVDKNV
                     INLNENELTYTLHVSMFSGTRNTLVIEDVLEEGMEFVSFLSDIPSFIDAEVNNGIVRF
                     SANNTIGGPTSFTLSFKVNVEAYAVGDQILNFATLVNSDNRLNTSTVRTRKIDGRIKV
                     IKTDENGQRLAGAKFEILSSDSQVIQEGITAENGEFLSEPLPVGSYQVRETEAPEGYL
                     LDATVHDVFINEYSIEEVTLTIENILDTGGVELIKHAEGSGEVLQGAVFELQNREGET
                     LQTGLTTGEDGKLAIDGLAPGAYQLVET"
     gene            complement(2975..3275)
                     /locus_tag="IV334_002693"
                     /pseudo
     CDS             complement(2975..3275)
                     /locus_tag="IV334_002693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013720767.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="IS607 family transposase"
BASE COUNT          895 a          738 c          535 g         1107 t
ORIGIN      
        1 gtttctacca gctgatatgc tcctggtgct aaaccgtcaa ttgccagttt tccatcttct
       61 cctgtagtta aaccggtttg tagcgtttct ccttctcggt tctgtaactc aaaaacagct
      121 ccttgaagga cttcaccact gccttcagca tgtttaatta gttcaacacc acccgtatca
      181 aggatgtttt caatggttaa cgtcacttct tctatggagt attcatttat aaaaacatca
      241 tgtaccgtcg catctaaaag atacccttcc ggtgcttctg tttctcggac ttgatagcta
      301 cctacaggaa gcggttcact taaaaattct ccgttttctg ccgttatacc ttcttgtatc
      361 acttgtgaat cgcttgataa aatttcaaat tttgctcctg ccagcctttg gccattctca
      421 tcagttttaa ttactttaat acgcccatcg attttccttg ttcttacagt actcgtattc
      481 aatcgattgt cactattcac aagcgttgca aagtttaaaa tttgatcgcc gaccgcatac
      541 gcctctacat tgaccttaaa agagagggta aaacttgtcg gaccaccaat cgtattattc
      601 gcactgaaac gaactattcc attattgact tcggcatcaa tgaaagaggg aatatctgat
      661 agaaatgaca caaattccat gccttcttcc aaaacatctt caataacaag cgtatttctt
      721 gttccagaaa acatcgagac atgcagagta tacgttagtt cattttcatt gagattgata
      781 acatttttgt ccacactttt tcttaaagag attgccggaa gattactttc agggattctc
      841 aagcggtagt ctcggctcat tacctgatta ttgtaggtca tactcgctcg attccgagaa
      901 aacccatcct cttgtcttcc ttcattgaac aagtcgacat cggtcacttc aagagcatat
      961 tcaataaaat acgattgacc ctctgctaga ttaccaaagt ccacaacaag tgtattcgtt
     1021 tccttattaa aagatacttt gtccacaaat tcctcggaaa cataatgagt tctaacgttt
     1081 gcttcacctg gattcgttac ttctgcgatc cgaaccccac ctgaaatagg ttctctattt
     1141 ccagtggaac tccatgtagt cggattcggt gccaaagttg ttccttgcgg tagtacatcg
     1201 gtaaccacta caccgtcttt tcgtgatcct tcataattta cctgcaaaat aaatttatta
     1261 ctgttggtca cgtcttgccc gtcaatcagc agattcccaa gatttccttg gctgtcgatt
     1321 tctctagagc catagtataa tttcgaaaaa atgggtcttg atgaaggaat aggcgtaatt
     1381 gaaaattccg tgtttgctgc tcgctcagca taagtaagac tcacttgaaa gtcattattt
     1441 ggctcagtac cgcctgattg aaagaaagca acttcaaacg tgatactgaa tgtatacgac
     1501 tgagcggctt catttaatcc ttcataatca aaggtaaatg gaaaaacaac gtctgtgcct
     1561 tcttggtaaa atgccagtgg ctttaaaggt gaatcatcag cgacctccac tctaatgaac
     1621 tcaattaagc caatcgatgc tctaggaact cgtacgacca tctccccata ttcttcaaga
     1681 atatcgccag cgcctccttg aatgtatccc tttaacgttt gtgtatgcct gctcggcaaa
     1741 gtggggcgct cagcagagat gccgatgtca taattcaaga gctcctcttg tctttcaatc
     1801 tgagattcct gcgttataga tggctcagat gatctggctt cattaaaaga ttcacttaag
     1861 ctttcttctg gtatattaaa agattcactt gtgctttctt ctggtacatt aaaagactca
     1921 cttgtgcttt cttctggtac actgatttct attgagaccg tatttgattg aatatcaccg
     1981 cttcttgcgg tcaattcgta tttacctgaa tgctgggaag aataagtaat cagacgttcg
     2041 taatcattga gttgcttcac ttccccttca atcaaatcct cactcttatc aatgaccaat
     2101 gcatttagca ggtctggtgt tcctctgact cgcaactgga actgctcacc caaagcagcc
     2161 tcctcttgat caacttcgat cgtcagcgaa cgctctggct gttctgctaa agactcagct
     2221 agaaccgtta tgttcccaaa ttgattgatc acaataagca taactagaaa catacttaac
     2281 agtttatgaa atttgttcct cactattact cccccttata attttgacta aacaacaagg
     2341 tacccgataa ttttctaaaa agattcccca actcaaaatg gctgcgttac ctccctcgta
     2401 ttttcggttc aactcaagcg ttgcaatgta agctgtaaac aaagctaatg attgccgatt
     2461 gtcttctgtt gattgattcg atagctttgt atgactttta gttgtccgtg tggcttaacc
     2521 ataattatga tttcacgatt cctcctgcca gtgccttgca caagctggag cgtaacccta
     2581 atagttattt tgttaatgta cttatatact tttatacttt tccattagaa acaatcacta
     2641 aatcgttgat accaaggtca gactctagaa tttcatcagt gaatccctgt attggttaac
     2701 cttcatcaat cccaaaaaaa tatatcaatg cttagcatca aaatgaatat atagtttaga
     2761 atacttggga ttcatcgata actgatcgct tatttggcca tattatttgg cacttggttc
     2821 aactaaacgc atttaggatg ttccttcatt ctcgtcatgg atgttctcaa accgtcatta
     2881 ctaataaact gtccaccgaa tcgatggttc atttcttgca ttttccacgt ccaattactc
     2941 tttttgctct ttatttgggc gtagtttcac tttcttaact tgtatcgtct tccatcaact
     3001 ccttaatcat cttcttagct ttattggctc ggtttccttg taaaaggtat ctacaaatag
     3061 tgacaatctg tattaaatct tcatcatttt ctgtaatgtc aataatttct atattctgta
     3121 ccatatattg caaagggttt cactaagttc ataaccgaag cgaattaaac aacttttgta
     3181 tagaataact accttttcta tttttccatt ggtaattcta tctattaatt ggtttaatcc
     3241 ttttgttgta gttgattcca ctaccaagat gattc
//
LOCUS       DACDKM010000167         3204 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20834_2016
            SAMN12640370-rid9891663.denovo.169, whole genome shotgun sequence.
ACCESSION   DACDKM010000167 DACDKM010000000
VERSION     DACDKM010000167.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640370
            Sequence Read Archive: SRR10040891
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3204)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3204)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742835.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742835.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:04:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,012
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 29 of 75
            Pseudo Genes (incomplete)         :: 33 of 75
            Pseudo Genes (internal stop)      :: 19 of 75
            Pseudo Genes (multiple problems)  :: 5 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3204
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640370-rid9891663.denovo.169"
                     /isolate="E20834_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(<1..758)
                     /locus_tag="IV334_002694"
     CDS             complement(<1..758)
                     /locus_tag="IV334_002694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1182-like element ISEfa7 family transposase"
                     /protein_id="HAP8944702.1"
                     /translation="MQGVIAMMSKNNTNGRNQFAMLTIDDLVPQDHLVRKIDAALDFE
                     FIYPIVEATYSDLGRPSIDPVILIKLVFIQYLFGIRSMRQTIKEVDTNVAYRWFLGYS
                     FEEKIPHFSTFGKNYVRRFRETTVFEDIFAYILEQAVKAGFVTEDNLYLDSTHIKANA
                     NKHKFTKEMTHDEAKAYQDELEDEINRQRIEAGKRPFTLDLEKEVKLKERKISKADPE
                     SGYYVKGEREKQFAYSAHTSCDDNGFILSTIITPG"
     gene            complement(730..1929)
                     /locus_tag="IV334_002695"
     CDS             complement(730..1929)
                     /locus_tag="IV334_002695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002389929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HAP8944703.1"
                     /translation="MARKKRNPDAEKLAESILNTYQPESVEDMQDVLKDVFGPLFEKM
                     LQGELNNHLGYDAHSKEPKKHDNRRNGYGNKTLKTSFGKVNIDVPRDREASFEPELIP
                     NRKRDVSDIEGKVLSMYARGMSQCDIAATVEDIYGFDISHEMISDITDAVLPELEEWQ
                     ARPLAKCYAFLFVDCMYVTLRENYEAKEYAVYTILGYDLKGNKEILGLWLNQTESKNR
                     WMQVFDELKVGGVEDVFFISMDGVSGLKEGAKAIFPSVIVQRCIVHLVRNALRYLPSK
                     DYKEVCRDMKKFYSASSLNAAHAAFDSFQNRWSHYSGAVDVCKRNFSHVEQLFDYGSA
                     IRKIMYTTNAVESIHSSFRKVTKKGVFSNENAQTVDKVHFMDDVYSLFFYNRFMKTNA
                     RSDSYDV"
     gene            complement(2059..>3204)
                     /locus_tag="IV334_002696"
     CDS             complement(2059..>3204)
                     /locus_tag="IV334_002696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP8944704.1"
                     /translation="DIKKISQTDSIDSYVKRINSVADLVDYDIIETQETLANQSPERA
                     KNFKRTVMLTGVNDSTKETKFVSEAYKLVEGKHLENEDKNKILMHKDLAEKNNLKVGD
                     KIKIKSNLFDADNEKGADETVEVEIKGLFDGHNSGGVSAAQELYENTLITDIHTAAKV
                     YGNTEDTAVYQDATFFVKGDKNLDSVIKDLGKLDINWREYNLIKSSSNYPALQQSISG
                     IYSISNKLFFGSLIFAGVVVSLLLFLWMNARKKEIAVLLSLGISKLEIFGQFLIEMVF
                     ISIPAFVGSYFLAQYTADKLGNNILNKVTGDIAKQIAKQSASSQLGGGAEAEGFNKTL
                     SSLDINVLPKFMIYVVIFMSLVLLVSLIISSFNILRRNPKELLIDNN"
BASE COUNT          965 a          625 c          506 g         1108 t
ORIGIN      
        1 ccaggagtga tgattgtgct aagaataaat ccattgtcgt cgcatgaagt atgggcagaa
       61 taagcaaatt gtttttctcg ttcgcctttg acgtagtagc cgctttccgg atcagcttta
      121 cttatttttc tttctttcag cttcacttcc ttttctaagt ccaaagtaaa agggcgtttt
      181 ccagcctcta ttcgttgacg attgatctca tcttctaatt catcctgata ggctttcgct
      241 tcatcatggg tcatttcttt cgtaaactta tgtttattgg cgttagcttt gatgtgggta
      301 gaatccagat acaaattatc ctctgttaca aaacctgctt taactgcttg ttctaaaata
      361 taggcaaaaa tatcctcaaa caccgtcgtt tctctaaatc ggcgaacata attctttcca
      421 aaagttgaga aatgggggat tttctcctcg aaggagtagc ctaagaacca gcggtaagcg
      481 acattggtat cgacttcctt aatggtttgt cgcatggaac ggataccgaa taaatactga
      541 ataaacacca atttgatcag aatcactgga tcgatacttg ggcgacctaa atcggagtaa
      601 gtggcttcga cgattggata aataaattca aaatcaagcg cggcatcaat ctttcgaacg
      661 agatgatctt gaggaactag atcatcaatc gtaagcatcg caaattgatt tctaccattc
      721 gtattgtttt tagacatcat agctatcact ccttgcattc gttttcataa atctattata
      781 aaagaaaaga ctgtagacat catccataaa atggactttg tctacagtct gcgcattctc
      841 attagagaac actccctttt ttgtgacttt tctgaagcta gagtgaatac tttcaactgc
      901 attagtagtg tacatgattt ttctaatggc gctgccataa tcaaagagtt gttcaacatg
      961 agaaaagttt cgtttgcata catctactgc accagaataa tgagaccacc gattttgaaa
     1021 actgtcgaag gcagcgtgtg cagcgtttaa agaagaagca ctgtagaatt ttttcatgtc
     1081 tcgacagact tccttataat ccttacttgg gaggtatcgt aacgcatttc gaacaagatg
     1141 aacaatacag cgctgcacaa tgacagaagg aaaaattgct tttgcgcctt ctttgagacc
     1201 ggaaacaccg tccatcgaaa tgaaaaaaac atcttctaca ccacctactt tcagttcatc
     1261 aaatacttgc atccagcgat ttttagattc tgtttgattg agccataggc ctaaaatctc
     1321 cttgtttcct ttaagatcat agccaagaat ggtgtacaca gcatattctt tagcttcata
     1381 gttttcgcgt aaagtgacgt acatacaatc aacgaataga aatgcataac acttggctaa
     1441 tggccgagct tgccattctt ccaattcagg aaggacggca tccgtgatat cagaaatcat
     1501 ttcgtgggaa atatcaaagc cgtaaatatc ttcaacggtt gcggcgatat cgcattgact
     1561 cattcctcgt gcatacatgg aaagaacctt accttcgatg tcggagacat ctcgttttct
     1621 attaggaatc aactcaggtt caaaagaagc ctcccggtct ctgggaacat caatgtttac
     1681 tttaccaaag ctcgttttaa gcgtcttatt gccataaccg tttcgtcgat tgtcatgttt
     1741 ctttggctct ttagaatggg cgtcataccc taaatggtta ttcaattctc cttgaagcat
     1801 tttttcaaat aagggaccaa acacatcttt taaaacatct tgcatatcct ctacagattc
     1861 gggttgataa gtattcaaaa tggattcagc taacttctca gcatcaggat ttcttttctt
     1921 tctagccatc gtgtaatcac ctcttgatct tattttagaa aaataaaaac cgtgaagcaa
     1981 ccacccgcct aggattgatg gttacttaca cggtttatat tacactctca tttttggtgc
     2041 taaaaagcac cgaaaatttt aattattgtc aattaataat tcttttggat tccttcttaa
     2101 tatattgaat gaagaaataa tcaatgatac aagaagtact aaactcataa atataactac
     2161 ataaatcatg aatttaggta atacattgat atctaaactt gataatgtct tgttaaatcc
     2221 ttcagcttct gccccaccac ctaattggct agatgcagat tgtttagcta tttgtttagc
     2281 tatatcacca gtaaccttat tcaatatatt attcccaagc ttgtcagctg tatattgagc
     2341 taagaaatag gaaccaacga atgcggggat tgatataaat accatctcaa ttaggaattg
     2401 accaaaaatt tctaattttg atatacctaa tgatagtaat actgctattt ctttctttct
     2461 agcattcatc cataagaata ataataatga aactacaact cctgcaaata ttaatgagcc
     2521 aaaaaataat ttatttgaaa ttgagtaaat acctgatata gattgttgta atgcagggta
     2581 atttgatgaa cttttaatca aattatattc tctccaattt atatctaatt tcccaagatc
     2641 ttttattaca ctatcaagat tcttatcacc ttttacaaag aatgttgcat cttggtatac
     2701 agctgtatct tcggtattac cataaacttt agcagctgta tgaatatcag tgattaatgt
     2761 attttcgtat agttcttgtg ctgcacttac tccaccgcta ttatgcccat cgaatagacc
     2821 tttaatttct acttctactg tttcatctgc acctttctca ttatcagcat caaataaatt
     2881 agattttatt tttatcttat ctccaacttt aaggttattt ttttcagcta aatctttatg
     2941 cattaagatt ttatttttat cttcattttc taaatgcttt ccttctacta atttatatgc
     3001 ttctgataca aattttgttt cttttgttga gtcgttaacc ccagttaaca taactgttct
     3061 tttaaaattc tttgctcttt caggtgattg attcgcaaga gtctcttgag tttcaataat
     3121 atcataatca actaaatctg caacactgtt tattcttttt acataggagt ctatactatc
     3181 tgtttgagat atctttttaa tatc
//
LOCUS       DACDKM010000168         3199 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20834_2016
            SAMN12640370-rid9891663.denovo.170, whole genome shotgun sequence.
ACCESSION   DACDKM010000168 DACDKM010000000
VERSION     DACDKM010000168.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640370
            Sequence Read Archive: SRR10040891
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3199)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3199)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742835.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742835.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:04:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,012
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 29 of 75
            Pseudo Genes (incomplete)         :: 33 of 75
            Pseudo Genes (internal stop)      :: 19 of 75
            Pseudo Genes (multiple problems)  :: 5 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3199
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640370-rid9891663.denovo.170"
                     /isolate="E20834_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..565
                     /locus_tag="IV334_002697"
     CDS             <1..565
                     /locus_tag="IV334_002697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="3-hydroxy-3-methylglutaryl-CoA lyase"
                     /protein_id="HAP8944705.1"
                     /translation="EEYMEELRKFLSDKKILLIAPGKSSVDEVERIKEFVTKEEVVII
                     SVNFEYSHIDIDYVFLSNLRRFHELPKESRTKCIVTSNIQASDVYLRTSYKKLLSERE
                     VVRDNAGLMAIRFLADMNVDEIFLAGFDGYSHNEDENYGEQSMEIITKFALLDAMNVQ
                     MRDELSELAKKVKVTFLTDPRWVRIDK"
     gene            700..1278
                     /locus_tag="IV334_002698"
     CDS             700..1278
                     /locus_tag="IV334_002698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8944706.1"
                     /translation="MISILCIVAYHANLRLNYNADGIIVHGSLTANQIFLNYIGIWGQ
                     LGVNCFIFISAYFLSEKRNIHFENVLGIVLECIFYSVLVYGILCACGIETFSLNSFVK
                     EILSFALGRYWFITSYLALYILSPYLNIMLNNLEKLQLQKISMLLFIGVCCYRMLFKT
                     SLFGDCAVLIALYVICYTLKKTIIIFLRDTES"
     gene            1224..1742
                     /locus_tag="IV334_002699"
     CDS             1224..1742
                     /locus_tag="IV334_002699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8944707.1"
                     /translation="MLYTEKNDNNIFERHRIIGTIVCITVIPLLISIGILFKFIPNAM
                     VNQMVHIGSPLMVIASIFLFYVFKNFSFYSKYINKLARTSLGVYILHENHYFIDGMKS
                     RGLIWDGIFGLGKRFLDRDFWIWFIIAVVVVCIICMAIDVVREKFERQIFKIKSVKNL
                     CKLMNHVINIYP"
     gene            1750..2790
                     /locus_tag="IV334_002700"
     CDS             1750..2790
                     /locus_tag="IV334_002700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HAP8944708.1"
                     /translation="MRRKEVKVISFGELLLRLAAPGYSKLFQRDSLETTFCGGEANVA
                     VSLAIFGYQSEFITKLPDSDVGYAAANALRYFGVNVDNIIFGEGRMGLYYLEKGAAQR
                     PSKVIYDRKFSAISLADSDEFKWKEILNDANWFHWTGINPALSENLFETSLDACQAAK
                     KRGITISCDLNYRSKLWTSEEAKEKMEQILPLVDICICNEEDAEKVLGIKAEHTNINS
                     GELNKDGYVQVAREIHKKFGCRYVATTLRRSYSASRNGWSAMLYEAEKDKAYFSTEYN
                     IQIVDRVGGGDSFSAGIIYALCENMSGQKAIEFATASSCLKHTIEGDFNRTTIEDVYN
                     LMNSGGNGRVLR"
     gene            3039..>3199
                     /locus_tag="IV334_002701"
     CDS             3039..>3199
                     /locus_tag="IV334_002701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HAP8944709.1"
                     /translation="MTYTYLTTDELVIIESYLKINQSVAKTAHCLNRSSQTIHKVYLF
                     FKQRKSALE"
BASE COUNT         1141 a          356 c          643 g         1059 t
ORIGIN      
        1 ggaggaatat atggaagaac tgagaaaatt tctctcagac aaaaaaattc tactgattgc
       61 accaggaaaa agttctgtag atgaggtaga aagaataaaa gagtttgtta ccaaagaaga
      121 agtcgtaata attagtgtaa attttgaata ttctcatata gatatagatt acgtattttt
      181 aagtaattta agacgttttc atgaattacc gaaagaaagc agaactaaat gtattgttac
      241 atcaaatata caagcatcgg atgtatattt acggacgagt tataaaaagt tgttatctga
      301 aagggaagtt gtaagagaca atgcaggatt aatggcaatt agatttttag ccgatatgaa
      361 tgtggatgaa atattcttag ctggatttga tggatattca cataatgaag atgagaatta
      421 tggtgagcaa tccatggaaa ttataacaaa atttgcatta ttagatgcaa tgaacgtgca
      481 aatgagagat gaattgtcag aattagctaa aaaagtaaag gtgacatttt taacggatcc
      541 acgttgggtt cgaattgata agtagtagta ataatgtttt taaaattttg ataaagagat
      601 agtgagagaa taaaaatggg taaagtctta aataaaaaag aagaaaaata tttaagggac
      661 gatggggcga gatgaaactc taattttgaa cttttaagaa tcatttctat attgtgtatt
      721 gttgcttacc atgctaattt gcgtttaaat tataatgcag acggaattat agtacatggt
      781 tcattaactg ctaaccagat tttcttgaat tatattggta tttggggtca gctaggagtt
      841 aattgtttta tatttatttc ggcatatttt ttaagtgaga aaagaaacat tcactttgaa
      901 aatgttttgg gaattgtatt agaatgtatt ttttatagtg tattagttta tggaattctt
      961 tgtgcatgtg gaatagaaac attttcattg aacagctttg taaaagaaat attatcattt
     1021 gctctaggta gatattggtt tattacatcc tatttagcat tgtatattct aagtccgtat
     1081 ttaaatatca tgcttaacaa tctggaaaaa cttcagttgc aaaaaataag tatgttattg
     1141 tttataggtg tatgctgtta tcgtatgtta tttaaaacat ctttgtttgg ggattgtgct
     1201 gttttaattg cactatatgt catatgttat acactgaaaa aaacgataat aatatttttg
     1261 agagacacag aatcatagga acaattgtat gtataacagt tataccactt ctcatatcca
     1321 taggaatact tttcaaattt ataccaaatg caatggttaa ccagatggtt catattggtt
     1381 ctccattgat ggttatagca agtatatttt tattttatgt atttaaaaat ttttcatttt
     1441 acagtaagta tataaataaa cttgcgagaa ccagtttagg ggtatatatt ttacatgaaa
     1501 atcattattt cattgatggt atgaagtcta gaggcttaat atgggatggg atatttggat
     1561 taggcaaaag gtttttagat agggattttt ggatttggtt tataattgca gttgtagttg
     1621 tgtgcataat ttgcatggct atcgatgttg taagagaaaa atttgaaaga cagattttta
     1681 aaattaagag tgtaaagaac ctgtgtaaac ttatgaatca tgttataaat atatacccat
     1741 aattaataaa tgaggagaaa agaagtgaaa gttatatcat tcggagaatt attattgaga
     1801 cttgcagcac ccggttattc aaagttattt caaagagata gtttggagac tacattctgc
     1861 ggaggggaag ctaatgtagc ggtatcctta gcaatatttg gatatcaatc agaatttata
     1921 acaaagcttc cggattcaga tgttggatat gcggcagcta atgctttgcg ttattttgga
     1981 gtaaatgttg ataatattat atttggagag ggacgcatgg gactgtatta tttagaaaaa
     2041 ggagcagctc agagaccatc taaagtaatt tatgacagaa aattttctgc aatttcgtta
     2101 gctgatagtg atgaatttaa gtggaaggag atcctaaacg atgcaaattg gtttcattgg
     2161 acagggataa atccagcatt gtctgagaat ctctttgaga cgtccttaga tgcatgtcag
     2221 gctgccaaaa aaagaggtat aacaatttct tgtgatttaa attatcgatc caagctttgg
     2281 acatcagagg aagcaaaaga aaagatggaa cagatactac cattggttga tatttgtatt
     2341 tgtaatgaag aagatgcaga aaaagtgcta ggaattaaag ctgagcatac aaatattaat
     2401 agtggagaat taaataaaga tggctatgtg caggtggcta gggagattca caagaagttt
     2461 ggatgtaggt atgtagctac aactctgcgt agaagttatt cggcaagtag aaatggttgg
     2521 agtgcaatgt tatacgaagc agaaaaagat aaagcgtatt tttccacaga atacaacata
     2581 caaattgttg atcgcgttgg tggtggagac agcttttctg ctggtataat atatgcgtta
     2641 tgtgaaaata tgtccggaca aaaggctatt gaatttgcga ctgcatctag ttgtttaaaa
     2701 catactatag aaggtgattt taatcgaact acaattgagg atgtatataa tttaatgaat
     2761 agcggtggaa atggtcgcgt gttgcgttag tcaacatata aagataaatc acagtaattt
     2821 ttatggtgat tatctgtctt agtagtgatt agaataacgt gaaaataaat tgttgagcgt
     2881 ttatactgtt ttgaaaaata aatttaacat attgaaaaat acaaatatat acgtatatta
     2941 taaagcgccg attgtaaaat taagctagac aactaaaaca gtcatttgtg ctacactcaa
     3001 aatagttccg accaaagaat tataaggagt gagtgcaaat aacctatacc tatcttacaa
     3061 cggatgaact tgtaataata gagtcttatt tgaaaattaa ccaatctgtt gctaaaactg
     3121 cgcattgctt gaatcgttca agtcaaacga tccataaagt atatctgttc ttcaagcaaa
     3181 gaaaatcagc cttagaata
//
LOCUS       DACDKM010000169         3155 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20834_2016
            SAMN12640370-rid9891663.denovo.171, whole genome shotgun sequence.
ACCESSION   DACDKM010000169 DACDKM010000000
VERSION     DACDKM010000169.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640370
            Sequence Read Archive: SRR10040891
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3155)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3155)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742835.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742835.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:04:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,012
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 29 of 75
            Pseudo Genes (incomplete)         :: 33 of 75
            Pseudo Genes (internal stop)      :: 19 of 75
            Pseudo Genes (multiple problems)  :: 5 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3155
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640370-rid9891663.denovo.171"
                     /isolate="E20834_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            94..783
                     /locus_tag="IV334_002702"
     CDS             94..783
                     /locus_tag="IV334_002702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HAP8944710.1"
                     /translation="MDKKRFLDLISKGLIVSCQALPGEPFYKEEESLMPYFALAAQRA
                     GAVGIRANSVRDIIEIRKCVSLPMIGIIKRDYPPESPFITATMREVDELVETGVEVIA
                     LDCTLRNRHDGLSINSFITKIKEKYPHQLFMADISTFEEGKNAYKAGIDFVGTTLSGY
                     TEESKKQDGPDIDLIKKLVEEGICVIAEGRIHTPEQAKEIKKIGVAGIVVGGAITRPQ
                     EIASRFVKALS"
     gene            797..2299
                     /locus_tag="IV334_002703"
     CDS             797..2299
                     /locus_tag="IV334_002703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010759843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP8944711.1"
                     /translation="MFKKFSQLGRAFMLPIAILPVAGLLLGLGGALTNESAINAYPFL
                     DQPWLHTILSIMSYAGNAVFANLALIFAIGIAVGLANGDKGTAGLAGGVAYLVYTATI
                     SGFLELFSAKDATLDTGVVGSIVVGGTVAFLHNRYRKIELPQFLGFFGGSRFIPIVSS
                     LAAIIIGSFFYLIWPPIQNGLVVAGEQIAQMGSLGTFLYGFLLRLTGAVGLHHTIYPL
                     FWYTSLGGTETVAGSTIAGAQNIFFAQLADPNHTGLFTYGTRFFAGRFATMMFGLPAA
                     CYAMYRAIPKKNRKKNGGLYFSGALTSFLTGITEPVEYMFLFVAPWLYVIHAFLDGLS
                     FYFADILNIRVGNSFSGGLIDFLLFGVLQGNDKTNWLKLIPFGIAWAFIYFFVFTFFI
                     KKFKVAIPGMEEESEVHSEKVINKNTSLHEESLIIIEALGGKENIESVEACATRLRVA
                     VTNGEIIQKQTIKDTGATAVFEVKGGIQAVFGGKADLLSQEINQILGKDN"
     gene            2312..2788
                     /locus_tag="IV334_002704"
     CDS             2312..2788
                     /locus_tag="IV334_002704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HAP8944712.1"
                     /translation="MFEIFKKKKQETFYAPCSGKIISITEVNDPVFSQKLLGDGFAVI
                     PDGSKIYAPLAGRVTNIFPTKHAINIKSNSGIDYLIHIGIDTVELQGEPFSICVQEND
                     EVTPETSLVDVDFDKIIKSGKDTSVIVVFPEKNQFNDLIIDNGRIAHGNSCGKITF"
     gene            complement(2885..2995)
                     /locus_tag="IV334_002705"
                     /pseudo
     CDS             complement(2885..2995)
                     /locus_tag="IV334_002705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252604.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         1035 a          422 c          618 g         1080 t
ORIGIN      
        1 ctccattttt atctttactt ttatgaaagc ggatgctata ttgtttttgg agttgttatc
       61 caaaaataat aaaaaattgg agtgtttagg ataatggata aaaagaggtt tttagatctt
      121 atttcaaaag gtttgattgt ttcctgtcaa gcattacctg gtgaaccatt ttacaaagag
      181 gaagaaagtt taatgcctta ttttgctttg gctgctcaaa gggctggagc agttggtata
      241 cgtgcaaatt ctgttcgaga tattattgaa attagaaagt gtgtatcatt accaatgatt
      301 ggaattatta aaagagatta tccaccagaa tctccattca ttactgccac tatgagagaa
      361 gtagatgaat tagtagaaac cggtgttgag gtaatcgctc ttgattgcac tcttagaaat
      421 cgacatgatg gactatctat aaattctttt attactaaaa taaaagaaaa atatcctcat
      481 cagttattta tggcagatat ttctacattt gaagaaggca aaaatgcata taaagcagga
      541 atagattttg taggaactac tttaagtgga tatactgagg aaagtaaaaa acaagatggg
      601 ccagatatag atttgattaa aaaattggta gaagaaggga tttgtgtaat tgctgaggga
      661 aggattcata ctccagaaca agcaaaagag ataaaaaaaa taggagttgc aggtattgtt
      721 gtgggtggtg ctattactcg accacaggaa attgcatcaa gatttgttaa agcattaagt
      781 tagggggaga aataaaatgt ttaaaaaatt ttcacaatta ggacgcgctt tcatgttacc
      841 aattgctata ttaccagtag ccggattact attgggactg ggaggagcgt taacaaatga
      901 gagtgctata aatgcttatc catttttaga tcaaccgtgg ttgcatacaa ttttaagtat
      961 tatgagttat gctggaaatg cagtttttgc aaacctagca ctaatttttg caattggtat
     1021 tgctgtagga cttgcaaatg gagataaagg aacagctggt ttagctggtg gagtagctta
     1081 tctagtatat actgcaacta ttagtgggtt tcttgaactt ttttcagcaa aagatgcaac
     1141 actagataca ggtgtagtgg gatcaattgt tgttggtggt actgttgcat ttttacacaa
     1201 tcgttataga aaaattgaat taccacaatt cttaggtttt tttggaggat caagatttat
     1261 tccgattgtt tctagtttag ctgcaatcat tattggttct ttcttttatt taatttggcc
     1321 accaattcaa aatggtttag tagttgctgg tgaacaaatt gcccaaatgg gtagtttagg
     1381 aactttttta tatggttttt tattgcgttt aacaggtgct gttggactac accacaccat
     1441 ttaccctttg ttctggtata catctttagg tggtaccgaa actgtagctg gatcaacaat
     1501 tgctggagcc caaaatattt tctttgccca actagctgat ccaaaccaca ctggtctgtt
     1561 tacttatggt actagatttt ttgcaggaag atttgcaaca atgatgtttg gattaccagc
     1621 agcatgttat gcaatgtatc gtgcaattcc taagaaaaat cgtaaaaaga atggtgggct
     1681 ttattttagc ggggccctta catcattttt aactggaatt actgaaccag ttgaatatat
     1741 gtttttattt gttgctcctt ggctttatgt gattcatgca ttcttagatg gattatcttt
     1801 ttattttgct gatatcttaa atattcgtgt agggaattcc ttttcaggtg gtttgatcga
     1861 ctttttatta ttcggtgtac ttcaagggaa tgacaaaact aattggttaa aattaattcc
     1921 ttttggtatt gcatgggcat ttatatattt ctttgttttt acattcttta tcaaaaaatt
     1981 taaggtagct attcctggaa tggaagaaga atcagaagtt cattctgaaa aagtaataaa
     2041 caaaaataca tcattacatg aagagtctct tatcattatt gaagctcttg gtggaaaaga
     2101 aaatattgag tctgttgaag cttgtgcaac tcgtttgcgt gttgctgtta cgaatgggga
     2161 gattattcaa aaacaaacaa taaaagacac tggtgcaact gctgtcttcg aagtgaaggg
     2221 tggcatccaa gctgtttttg gagggaaagc tgacttatta agtcaagaaa tcaatcaaat
     2281 attaggaaaa gataattagg aggtttttaa tatgtttgaa attttcaaaa agaaaaaaca
     2341 agaaactttt tatgcacctt gttccggaaa aatcatttcc atcacagaag taaatgatcc
     2401 tgtcttttca caaaaattac taggtgatgg ttttgccgtc atacctgatg gttcaaaaat
     2461 ttatgctcca ttggctggtc gagtaacaaa tatatttcct actaaacatg ctattaatat
     2521 aaaaagcaac tcaggcattg attatttgat tcatattgga attgatacag ttgaattaca
     2581 aggagagcct ttttcgattt gtgttcaaga aaatgatgag gttactcctg aaacctcgtt
     2641 ggttgatgtg gattttgaca aaataataaa atctggtaaa gatacttctg ttatagttgt
     2701 ttttccggag aaaaatcagt tcaatgattt aataatagat aatggtcgaa ttgctcatgg
     2761 gaattcatgt ggaaaaatca ctttttaaaa aagtgtatat aaaaagctat aaaataagct
     2821 aaataggata tttgtcaaat gaagttggtg aacagaaaga actgttttct ttctgttcat
     2881 ttttttattt tattagtaat gattccccat gccaaatcat gatccgtaat ttaaagttat
     2941 aaaagcttcg aaatccattg gcatttcgtt ggaatacctt gatatgatta ttcaaatata
     3001 aaatatagag agaagagaga gagggaagat atttataaag ggcaaagtta tgtttactca
     3061 acttaggaaa tagaaataga tcaatttata ttctcttgtc aatcgaatat gaaccttagc
     3121 actatatacc aaatatagta aaatgctgaa agatg
//
LOCUS       DACDKM010000170         3137 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20834_2016
            SAMN12640370-rid9891663.denovo.172, whole genome shotgun sequence.
ACCESSION   DACDKM010000170 DACDKM010000000
VERSION     DACDKM010000170.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640370
            Sequence Read Archive: SRR10040891
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3137)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3137)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742835.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742835.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:04:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,012
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 29 of 75
            Pseudo Genes (incomplete)         :: 33 of 75
            Pseudo Genes (internal stop)      :: 19 of 75
            Pseudo Genes (multiple problems)  :: 5 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3137
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640370-rid9891663.denovo.172"
                     /isolate="E20834_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            104..1021
                     /gene="glyQ"
                     /locus_tag="IV334_002706"
     CDS             104..1021
                     /gene="glyQ"
                     /locus_tag="IV334_002706"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HAP8944713.1"
                     /translation="MSKKLTVQDMILTLQKFWSSNGCMLMQAYDTEKGAGTMSPYTFL
                     RAIGPEPWNAAYVEPSRRPADGRYGENPNRLYQHHQFQVVMKPSPENIQELYLESLRL
                     LGIDPLEHDIRFVEDNWENPSMGCAGVGWEVWLDGMEITQFTYFQQVGGLPCKPVTSE
                     ITYGLERLASYIQEVESVYDLEWADGVKYGEIFKQPEYEHSKYSFEVSDQDLLLSNFD
                     RFEKEAKRCIDENLVHPAYDYILKCSHTFNLLDARGAVSVTERAGYLSRIRNMARAVA
                     KIFVAEREKLGFPLLNKNTANPENMKEAE"
     gene            1023..3098
                     /locus_tag="IV334_002707"
     CDS             1023..3098
                     /locus_tag="IV334_002707"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010728817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HAP8944714.1"
                     /translation="MAKDLLLEIGLEEMPAHVVTPSMKQLEEKTAKFLDENQLTYDTI
                     ETFSTPRRLAIKVTNIPERQADSEEEVKGPAKKIALDADGNWTKAAEGFVRGQGLTTD
                     AITFRELKGVEYVYVTKYTHGKEAKEVLTGLADVIKSLTFPVTMHWAEYDFEYIRPIH
                     WIVALLDDEVIPFEILDVKTGRASRGHRFLGEDVTFAHAKEYEEKLNEQFVIASPQKR
                     QEMIVTQANELAEKNHWVIDLDEDLLEEVNNLVEYPTVFSGDFEEKYLSVPEEVLVTS
                     MKEHQRYFEVRNDQGMLLPHFISARNGDRVKLENVVRGNQKVLTARLEDAEFFYNEDK
                     KASIDQYVEKLKTVTFHEKIGSIYEKMQRVQVIARIIGEQVGLSEEELMDLQRAAEIY
                     KFDLVTNMVGEFPELQGIMGEKYALLKGEKPAVATAIREHYMPISSEGELPQSTVGSV
                     LAIADKLDSIFSFFAVGMIPSGSNDPYALRRQAYGVVRIIESKKWAFPLSVLQETISD
                     VISKDTDRFGIGLSEGQQQVIDFIKGRLRQLLTTKNIRHDVIEAVLNAEQKDLTKVFA
                     AAQLFKQHLADEDFKPSMEALTRVVNLAKKAELEKQSEVDPELFENEAEKELHKAVEA
                     MKKSFADQTINENYDQLVSLRPLIENYFDQTMVMAEEPAVRKNRLTQLSQIAAMALSL
                     ASLDQIITK"
BASE COUNT         1056 a          583 c          680 g          818 t
ORIGIN      
        1 ttattcgtcc ctatgtctgt aatcagacat agggactttt tgtcgttttc tttcctgtat
       61 tcttattgac agcaacgctt aaaaattgaa aggggcaaaa cagatgagta aaaaattgac
      121 cgtccaagat atgattttaa cattacaaaa attttggtca tcaaacggct gcatgctgat
      181 gcaagcttat gacacagaaa aaggagcagg tacgatgagc ccatatacat tcttacgggc
      241 aatcggaccg gaaccatgga atgctgctta tgtagagcca tcccgccgac cagctgacgg
      301 gcgttatgga gaaaacccaa accgtttgta tcagcatcat caatttcaag tcgtaatgaa
      361 accttcacct gaaaatatcc aagagcttta tttagaaagc ttgcgtcttt taggaattga
      421 tccattagaa cacgatattc gttttgttga agacaactgg gagaacccat cgatggggtg
      481 tgccggtgtg ggttgggaag tatggcttga tgggatggag atcacacaat tcacgtactt
      541 ccagcaagtc ggaggactac cttgtaaacc agttacttct gagatcactt atggactaga
      601 acgtcttgct tcatatattc aagaagttga aagtgtctat gatctggaat gggctgatgg
      661 tgtgaaatat ggcgagatct tcaaacaacc agaatatgaa cattccaaat attcttttga
      721 agtaagtgac caagacttgc tactaagcaa ctttgaccgt tttgaaaaag aagctaaacg
      781 ctgtatcgat gaaaatcttg ttcatccagc gtatgattat attttgaaat gcagccatac
      841 attcaattta ttagatgctc gcggtgcagt atccgtaaca gaacgagcag gttatctttc
      901 ccgtatccgc aacatggctc gtgctgtagc caaaatcttt gtggcagaac gcgaaaaatt
      961 aggattccct ttattgaata aaaacacagc aaatccagaa aacatgaagg aggctgaata
     1021 agatggcaaa agacttatta cttgaaatcg ggctggaaga aatgcctgct catgttgtca
     1081 caccaagcat gaaacaatta gaagaaaaaa cagcaaaatt tttagatgaa aaccaattga
     1141 cttatgatac gatcgaaacg ttttctacac cgcgccgctt agcaatcaaa gtaacaaaca
     1201 ttccagaacg tcaagcagac agtgaagaag aagtcaaagg accagccaaa aaaattgctt
     1261 tggacgcaga tggaaactgg acgaaagcag ctgaaggatt tgtccgcgga caaggtctga
     1321 cgacagatgc tatcactttt agagaattaa aaggcgtgga atatgtctat gtaacgaaat
     1381 acactcacgg caaagaagcg aaagaagtat tgacaggttt agcagatgtc attaaaagcc
     1441 tcactttccc agtaacgatg cattgggcag aatacgattt tgaatatatc cgccctattc
     1501 actggatcgt ggcattgtta gatgacgaag taatcccgtt tgagatctta gatgtcaaaa
     1561 caggcagagc ctctcgagga catcgtttct taggcgaaga tgtgacattt gcgcatgcaa
     1621 aagaatatga agaaaaattg aatgaacaat tcgttattgc ttcaccgcaa aaaagacaag
     1681 aaatgatcgt tacacaagca aatgaattag ccgaaaaaaa tcactgggtt attgatctag
     1741 acgaagattt actagaagaa gtcaacaatt tagtcgaata tccaactgtc ttttctggcg
     1801 acttcgaaga aaaatatctt tctgttcctg aagaagtact agtcacttct atgaaagaac
     1861 atcaacgtta tttcgaggta cggaacgatc aaggtatgct gcttcctcat tttatttctg
     1921 ctcgtaatgg tgatcgggta aaattggaaa atgtggttcg aggcaatcaa aaagtattga
     1981 cggctcgttt agaagatgcg gaattctttt ataatgaaga caagaaagct tcaatcgacc
     2041 aatacgttga aaaactaaaa actgttactt tccatgaaaa aatcggtagt atctatgaga
     2101 aaatgcagcg ggtacaagtc attgcccgta tcattggcga acaagtagga ctatctgagg
     2161 aagaattgat ggatctgcaa cgagctgctg aaatctataa atttgatcta gtaaccaaca
     2221 tggtagggga atttcctgaa ctacaaggga tcatgggcga aaaatatgct ctgctgaaag
     2281 gcgagaaacc tgctgtagca acagcgattc gtgagcatta catgcctata tccagtgaag
     2341 gagagctacc tcagtctaca gtaggttctg ttttagcgat tgcagataaa ttagatagca
     2401 ttttttcttt ctttgcagtt gggatgattc caagcgggtc aaacgatcct tatgccttac
     2461 gtcgccaagc atacggtgtc gttcggatca tcgaatcgaa aaaatgggcc tttcctttgt
     2521 ctgttctgca agaaacgatt agtgatgtta tcagtaaaga tacggatcgt ttcggcattg
     2581 gtttatcaga agggcaacaa caggtgatcg actttatcaa aggccgtctg cgccaattat
     2641 tgacaacgaa gaatattcgt catgatgtca tcgaagctgt tttgaatgca gaacaaaaag
     2701 atctgacaaa agtatttgca gcagcacaat tgttcaagca gcatcttgca gatgaagact
     2761 tcaaaccatc gatggaagct ttgacacgtg tcgtgaactt agcgaaaaaa gcggagcttg
     2821 aaaaacaatc agaagtagac cctgaactgt tcgaaaatga agcggagaaa gaactgcaca
     2881 aagcagtaga ggcaatgaaa aaaagctttg ctgaccaaac gatcaacgaa aattatgatc
     2941 agcttgtaag ccttcgccca ctgatcgaaa attattttga tcagacgatg gtcatggcag
     3001 aagaaccagc agtacgtaag aaccgattaa cgcaattaag tcaaattgct gccatggctt
     3061 tgtcgctagc aagtttagat caaatcatca caaaataatt tctaatatgt aaataaacca
     3121 gagaataggc acaaaat
//
LOCUS       DACDKM010000171         3128 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20834_2016
            SAMN12640370-rid9891663.denovo.173, whole genome shotgun sequence.
ACCESSION   DACDKM010000171 DACDKM010000000
VERSION     DACDKM010000171.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640370
            Sequence Read Archive: SRR10040891
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3128)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3128)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742835.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742835.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:04:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,012
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 29 of 75
            Pseudo Genes (incomplete)         :: 33 of 75
            Pseudo Genes (internal stop)      :: 19 of 75
            Pseudo Genes (multiple problems)  :: 5 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3128
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640370-rid9891663.denovo.173"
                     /isolate="E20834_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            103..1347
                     /locus_tag="IV334_002708"
                     /pseudo
     CDS             103..1347
                     /locus_tag="IV334_002708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729806.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
     gene            1360..1992
                     /locus_tag="IV334_002709"
     CDS             1360..1992
                     /locus_tag="IV334_002709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HAP8944715.1"
                     /translation="MTMGVIFDMDGVLIDSETFYFERRMNFFQELEIDPGSNQIDDYV
                     GKTEAAIWETLVPEDKSLREELYPKYVDYRKTHPIDFAKALRKEVKPLLDFLKKRDIP
                     LALASSSPAVEIESMLIQCELTEYFSFVISGESLKQSKPHPEIYLKAKQQLDCDFFIA
                     VEDSTIGIKSAKAANIYTAALKQPMQIDQSEADIQIESLQELEQIVLEKG"
     gene            2197..2424
                     /locus_tag="IV334_002710"
     CDS             2197..2424
                     /locus_tag="IV334_002710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009320234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
                     /protein_id="HAP8944716.1"
                     /translation="MLQQNQNTNSYQAAILTAVNLGGDTDTTAAVTGALAGILYGGEV
                     IPENWRKSLQNDQLLKTISQRFCDTVLSQRI"
     gene            2493..2759
                     /locus_tag="IV334_002711"
     CDS             2493..2759
                     /locus_tag="IV334_002711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002383791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HAP8944717.1"
                     /translation="MLVQMMNGGRIMSQSTITFRIPDDEKELVLEYAKAHNSSLTELY
                     RNAVLDKIEDEIDLRTLRSAIEVSKEKKESGISQEEMEKLLNEL"
     gene            2749..3018
                     /locus_tag="IV334_002712"
     CDS             2749..3018
                     /locus_tag="IV334_002712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HAP8944718.1"
                     /translation="MNYKVIYMPQAQKQLKKMDRNQAKIIVAWIRKNLENTTDPRQHG
                     KGLTGNKSGEWRYRIGNYRILANICDSEIEIQIFSIGHRSTIYKR"
BASE COUNT         1101 a          466 c          617 g          944 t
ORIGIN      
        1 tgaaattgct ttcctttaaa atgactcatc gtcattcctc ctgctatctt tttctattat
       61 tctaccttat ctgatagtag atttaaaact ttgcaacaga accagaaaac caaagatcag
      121 cttaacgaat tttttgctaa tgttcttgaa accccttatt ggtatgcaaa aaaaatctta
      181 agccttttat ctgagtgttt agatcaacct gtgtatgaag aggacatttt ctttattacg
      241 ctatattttt atagtttgaa tgtcacgaat gttaaaacag gtattaagag tattgtgcta
      301 gctcatggtt attctacagc tagtagtatg gcgaatgttg ccaaccgtat gctgaataag
      361 aacgtttttc aagcttatga tatgccttta gatattacat tagagaaaat tgaagcaaaa
      421 ttgattcaat tccttggaga ttatcgaaca gattcaggct tgattttatt ggtagatatg
      481 ggttcactaa accaactagg cggtttatta gtgaatcatc taaatggacc actgttaatc
      541 attgatcata tcaatacacc gttattattg gaagtcggcc aacaagtatt aaacaataaa
      601 agtatcaccg aaataggagc agaaattgaa acaagtctta ctttcaaaaa gcagttacta
      661 cttcccaaga aaaagaaaaa gaaagccatc attacttgtt gttatacagg gattggtagt
      721 gcgacccaga ttcaggagat tttaacgaaa tgcttgaaag atgcagttag agaattagta
      781 attattccgt atgattacaa aaagctgtta gaaaacaaga attttgagac tccttttcaa
      841 ttatacgatg tcatcatgat tgtaggtaca gaagatccca aaattaatca gattccatat
      901 attggattag atcgcctgat taatggggga agcattggcg aatttgttca ttacttggaa
      961 aaagagctga ctattgaagt gaacgattta caaaaagaat taattttcaa tttttcaatt
     1021 agtaaaattg tcgaaaattt aaccattttg gatccagata aggtgctatc ttttgtacaa
     1081 aaagcaatta aagaattatc gaagttatta aattttgatt tttctaataa tcagctgttt
     1141 ttgttgtaca ttcactgtag ttgtatgatt gaacgaattg tacgtaaaga aagtgtggat
     1201 gagcaagagg acattgaaga atatatgcaa gcaaatggag aaataatgga taaaattcat
     1261 agcgcttttt ttgagattga aagggaatac acaattgaaa taccagattt agagttgcga
     1321 ttattgagcg atattgttca aggataggag tgaattcgaa tgacaatggg agtaattttt
     1381 gatatggatg gggtactcat agatagcgag acattttatt ttgagagaag aatgaacttc
     1441 tttcaagaac tggagataga tccaggatca aaccagattg atgattatgt ggggaaaaca
     1501 gaagctgcaa tatgggaaac attagttcct gaggacaaat cattaagaga ggaactttat
     1561 ccgaaatatg tagattatcg aaaaacgcat ccaattgatt tcgctaaagc tttacgaaaa
     1621 gaagtgaaac cgttacttga cttcctcaag aagcgggata taccattggc actggcatct
     1681 tcttctccag ctgtcgagat agagagtatg ctcattcaat gtgaacttac tgaatatttt
     1741 tcctttgtta taagtggtga gtcattaaaa caaagcaaac cacatcccga aatatattta
     1801 aaagcgaaac agcaattaga ctgtgacttc tttattgcag tagaggattc aacgattggt
     1861 attaaatcag caaaggcagc aaatatctat acggcggcac taaaacaacc aatgcaaatc
     1921 gatcagagtg aagcagacat acaaattgaa agtctacaag aactggaaca aattgtttta
     1981 gaaaaaggtt agtgaattaa tggaaataag gctatatatc tgtgctagct attttgacta
     2041 accaaaaaaa cttgctataa ttcaggtgta gtaaaaggtg ctggtaacac cttttacgaa
     2101 aatgcttcgg cagtgataat gggattggct ttttaagttt tttattttgt tcgcaatccc
     2161 taaggtcatg tgaatgatcc tagggattgc tagaatattt tgcaacagaa ccaaaatacg
     2221 aatagttatc aagcagccat attgactgcg gtcaatttag gaggggatac tgatactact
     2281 gctgccgtaa ctggtgctct tgcgggtatt ctttacgggg gtgaagtgat ccctgaaaat
     2341 tggagaaaat cgcttcaaaa tgaccagctt cttaaaacga tcagtcagag attttgcgat
     2401 accgtgctat ctcaaagaat ttgaataatg gatatgatgc aaatcttgtt tgtactaacg
     2461 ttgtattgac aaaaaggatt ttatttagta caatgttagt acaaatgatg aacggaggta
     2521 gaatcatgtc tcaatcaacc attacattta ggattcccga cgatgaaaaa gaactggtat
     2581 tagaatatgc aaaagcacac aattcttcat tgaccgaact ttatcgaaat gctgtactgg
     2641 ataaaatcga agatgaaatc gatctacgga ctttacggag tgcaattgaa gtttccaaag
     2701 agaaaaaaga gagtggtatt tctcaagagg aaatggagaa actgctaaat gaattataaa
     2761 gtcatctaca tgccgcaagc ccagaagcaa ttaaaaaaaa tggacagaaa ccaagctaaa
     2821 ataattgtcg cctggatcag aaagaattta gaaaacacga ctgacccgcg tcagcacgga
     2881 aaaggtttga ctggaaataa gagtggggaa tggcgctatc gaattggaaa ctatcgaata
     2941 ttggctaata tttgtgattc tgaaattgaa atccagatat tctcaattgg acatagaagt
     3001 acaatatata aaagataacc gtttttggaa cgaagtagag tttcgaaagt caatgtataa
     3061 tcttttgaat tttgcataat accacaggta gaaactaccc gatactatcg gttggtttct
     3121 gctttttt
//
LOCUS       DACDKM010000172         3089 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20834_2016
            SAMN12640370-rid9891663.denovo.174, whole genome shotgun sequence.
ACCESSION   DACDKM010000172 DACDKM010000000
VERSION     DACDKM010000172.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640370
            Sequence Read Archive: SRR10040891
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3089)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3089)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742835.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742835.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:04:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,012
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 29 of 75
            Pseudo Genes (incomplete)         :: 33 of 75
            Pseudo Genes (internal stop)      :: 19 of 75
            Pseudo Genes (multiple problems)  :: 5 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3089
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640370-rid9891663.denovo.174"
                     /isolate="E20834_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            1581..2548
                     /locus_tag="IV334_002713"
                     /pseudo
     CDS             1581..2548
                     /locus_tag="IV334_002713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002419316.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
     gene            2871..2987
                     /locus_tag="IV334_002714"
     CDS             2871..2987
                     /locus_tag="IV334_002714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010768364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HAP8944719.1"
                     /translation="MIGLNAISAKYCKFKAFVEKFTERQTDKIIQYHITYTD"
BASE COUNT         1165 a          414 c          561 g          949 t
ORIGIN      
        1 attatcgaac acctttggaa gtatttttga gttacgtaag tattgatgat ctgtctaatt
       61 taatttgaca attaagaaag tattaacttg ttttgtgaaa aagagaattt gaaaaacgat
      121 tggcgaacaa atgctcggta ggtatggtat aaatacagag ctaattggtg tgatgaaatc
      181 agatattcgc gtgagattca gtaaagtagg agttcgaaaa cagctaggtg acaaacagta
      241 ttgctttggt acttcggaat ggtttatcaa attcttatca acagttcact tctctttatg
      301 tttgagtgat gattcgttag gttattgctt ggaaagaccg gatgagagta gaagagttta
      361 attttataaa gtgaaaagag gtaatgtacg atatacgtag ggctgaacca atagaggata
      421 agcaaacgat gttacaggcg caaaattcat agcttactgt tttggagata acagaagagg
      481 aaataatgca gaagtaaggc gaaaaaagga cggaagccta acagcatatg aaataaagaa
      541 aaatattata actactgata aatcggtatt gagcaaagta ctaataggag tcaaaaacat
      601 tcataagcta tgagtgtttc ttggctactt ttttattttt atgtatctac aaaagtttcc
      661 aggggcagaa cctgtgatta ttaatggaag aacctcaagt attgtcttaa atcattgtta
      721 tgaaaatgga ttaaaagagt tcatcaaggt gtgattaaca agttactttt cattacatcg
      781 aatttttcag aagttgctta tattggtgat gatattaatg atttatcatg tatgacatct
      841 gtgaagaagc gggaggaata gtagaatgtc caaaaaatgc cacaagaaat gagattgaat
      901 tatctgattt tattgtaact cacagtggtg gtgacgaatc tgtgatagag tttattgagt
      961 ggatattgga atcgatatag tgtagtagta cagaaaaatt atcatacaat taatactagg
     1021 cataagaata ttggcatatt ctagagttaa aagtaactat tcgaaaagga taaggttaga
     1081 attttttgtt ttgccagtgg aagaatataa aaagggcatt ggaaattaat atattagaat
     1141 gctaagcgga gaaaaaaata tatgaaaagt acgtccttgt gatgggatgg aaaaatacac
     1201 ttatttcaaa caacgataat ataattatgt gaaccgaagt atactcgctt ctaattcaat
     1261 cctcaaatcc cgacagtcgc gacttcaaaa aagcgtatag ttttaatgta tccccatatg
     1321 atccgaaaaa tggtaattta caattaacag tttgatgatg ttgaagatgt ggaaatgctg
     1381 aaattaccat gatgagaaac acattaatta aagggaatta attaactaga atataatctg
     1441 attcaatttg aattaagcaa aaaaaggctg agttttaaat aatcgcgaat tgtaaaatta
     1501 agttagaaaa ataaaaaggc atttatggta cactcaaatt gtatttccga cgaaagaaaa
     1561 caaaggagtg taacccataa atgacctaca aacatcttac catagacgaa ctgacaatga
     1621 tagaatcata ttatcttcaa cataataagc cggttgaaat cgctaaccga atgggacgtg
     1681 ctatacaaac tatttataat gtagtcaata agttcaagca aggcaagact gctcttgatt
     1741 attggcacca gtataaagaa aataagaaaa aatgtggtag aaaagtcatt caattacctg
     1801 ctcatgaagt agattacatt aaagagaaag tcactcttgg ttggacgcct gacgtcatta
     1861 tcgggcgaaa agaaaggcct gtttcatgcg gtatgagaac actttatcgt ttattttcta
     1921 aaggaatatt tgatattgac acactaccga tgaaaggtaa aagaaaaccc aatggccatc
     1981 aggaaaaacg gggaaaacaa cagtatcagc gctcaatcca tgatcgacct gataattatc
     2041 ctcatttcaa ttctgagttt ggtcaccttg aaggtgatac gatcgttggc attcatcata
     2101 aaagtgccgt cattacttta gttgaaagat tatctaaagt cattatcacg attaaaccca
     2161 acggccgtaa ggcattagat attgaaactg cccttaatca atggttttct cgcttcccta
     2221 aaaacttctt taaatctatt acgctattac gtttgactgt ggaaaagaat tttctaactg
     2281 gaaagccatt agtaaccaac atgatattga tatatatttt gcggaccctg gaacaccttc
     2341 tcaacgccca ttaaacgaga attctaacgg gattctgcgt cgtaatggac tgccgaaatc
     2401 aatggatttt agagaagtga atcagacatt tatttccagt gtcagcaatc aacgtaatca
     2461 tattccaaga aaatcattga attacagaac accaatcgaa atatttttga gctatgtaca
     2521 agaagcattt tattctagct taatttgaca aatcataaat aataaagaat tgaacttctg
     2581 agaaaacaaa gaatcttttt tctttttttg aattgactca tggattggcg ttaaaatttt
     2641 attttgaaaa agttatttct ggtttaactc aaaaaaagag atatgaatct aatagtgata
     2701 gaaaggatgt tatttttcat gaaatggttg ttttagattt aatacatata actaatgaat
     2761 gctattgaat gtatttaaac taattaacaa aagaatttga attgttaata aaatccaata
     2821 aaatatatta ataaagtaga tagaaataaa aaaacaaaga ctaaacatat ataattggat
     2881 taaacgcaat atctgcaaaa tattgtaaat ttaaagcctt tgtagaaaaa tttactgaaa
     2941 gacaaactga taagattatt cagtatcata ttacctatac tgattaaaat tttttgaaac
     3001 gggatactaa ataaaaaatt tatttataac agaactaatt atttaatgcg gatgcgccaa
     3061 tacttggtcc tgcaagagct atttatttt
//
LOCUS       DACDKM010000173         3072 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20834_2016
            SAMN12640370-rid9891663.denovo.175, whole genome shotgun sequence.
ACCESSION   DACDKM010000173 DACDKM010000000
VERSION     DACDKM010000173.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640370
            Sequence Read Archive: SRR10040891
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3072)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3072)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742835.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742835.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:04:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,012
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 29 of 75
            Pseudo Genes (incomplete)         :: 33 of 75
            Pseudo Genes (internal stop)      :: 19 of 75
            Pseudo Genes (multiple problems)  :: 5 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3072
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640370-rid9891663.denovo.175"
                     /isolate="E20834_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(289..903)
                     /locus_tag="IV334_002715"
     CDS             complement(289..903)
                     /locus_tag="IV334_002715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HAP8944720.1"
                     /translation="MSLCYFDYYHQLEEKLRIEEPLMLQAGTWIAKQINQGGRLQIYA
                     SRTLSGVAFEFWDQTPDLVPSILIENPAGGVYETLEGAGQAIIDQLSVKSEDIFLLLS
                     NEGRNPAIIELAQWIKENGHPLIIVTGFDLSRSIKSQHSSGLRLFEFADLVLDNHANL
                     HDAVLTLPNLDPAICGTASLATIFLLQQVLYFTAKQLVQDTSGK"
     gene            complement(1509..1886)
                     /locus_tag="IV334_002716"
     CDS             complement(1509..1886)
                     /locus_tag="IV334_002716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8944721.1"
                     /translation="MGKLVAVAQSLLDILKWLMEGSNWIFVLPLLLISLFIMFRMVRK
                     KETFLIFIWYAASVGVATLMVELALGSWEACVFALFFAVLYLIIYIPYMKRKSRIGRK
                     GRHTTTFSSTDSLEQSRRTRRRR"
     gene            complement(2186..3067)
                     /gene="galU"
                     /locus_tag="IV334_002717"
     CDS             complement(2186..3067)
                     /gene="galU"
                     /locus_tag="IV334_002717"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HAP8944722.1"
                     /translation="MKVKKAVIPAAGLGTRFLPATKAMAKEMLPIVDKPTIQFIVEEA
                     LDSGIEDILIVTGKEKRPIEDHFDANIELENNLREKEKTELLALVEETTQVNLHFIRQ
                     SHPRGLGDAVLQAKAFIGNEPFVVMLGDDLMKDDIPLTQQLMMDYEETQASAVAVMRV
                     PENETSKYGIIDPKTDSEKGRCRVKGFVEKPEMGKAPSNLAIIGRYLLTPKIFEILET
                     QEPGAGNEIQLTDALQTLNQTEAVYAREFKGKRYDVGDKLGYMKTNIEYGLQHSEIGT
                     SLSAYIIQLSKKLRTES"
BASE COUNT          929 a          644 c          473 g         1026 t
ORIGIN      
        1 acaagaaaga atccatatgg attctttctt gtctcttttt ccactacttt ttatttttct
       61 gacactacat attcttataa ctcatttctc ataactgtga aacaacttat aaaaataaca
      121 aactttaaac aaaacaagct taaaatcaaa gatgatccat cttaaaataa ctaaaacaaa
      181 aaaagtagtc gttgttaatc ctcattaata atcgttttct ttttttcaaa cgctccattt
      241 ttatgtggaa cattattttt ttaaggaact ttgtcaacat gtggataact attttccact
      301 tgtgtcctgg actagctgtt ttgctgtaaa atacaaaact tgctgcagca aaaagatcgt
      361 tgcaagagag gctgttccgc agatcgctgg atctagattt ggcaaagtca agactgcatc
      421 atgtaaattg gcgtggttgt ccaaaaccaa atcagcaaat tcaaataatc gcaaaccact
      481 gctatgttgc gatttgatac ttcgtgacaa atcaaatcca gtaacaatga ttaatggatg
      541 accattttct ttgatccact gagctagttc gatgattgcc gggtttcttc cttcattcga
      601 aagaagaaga aaaatgtctt ctgatttgac agaaagctga tcaataattg cctgtcctgc
      661 accttctagc gtttcataga cacctccggc tggattttca atcaatatac ttggtacaag
      721 atccggggtt tgatcccaaa attcaaatgc tacaccactt agtgtgcggc tagcatatat
      781 ctgcaatcta ccaccttgat taatttgttt cgcgatccaa gtgccagctt gaagcatcag
      841 cggctcttct atccttaatt tctcttccaa ttggtgataa tagtcaaaat aacataaact
      901 catttatctc tcccgcctag ctatacttga catgaataga aacagctgcc taatctaagg
      961 aagtacataa aaaaacagta gttagacagt aacacatgtg gtctttgctg tttcaaacac
     1021 aaaaaaggca ttgaacgaat acgcccaata ccttgacata gcaaataaaa caacgtgcag
     1081 accatggaaa atatctacat aggggtagaa ttttcaccct agattaggta caataaatta
     1141 ggtcgatgca ctgcattgac tatggcaaaa tgatcggtca gggtgtatgt gtgtgactgg
     1201 tcattccctg attcttagcg ccaactaagg gtcagggagc ctttttttat tcagctgttt
     1261 ctatctgaat ttattgtaag taatctctta tccaagtgca agcaagaaaa tacaaaaaaa
     1321 cattggtatg atttatatat atataaataa cataaaaaaa ggaatggctt atcccgatac
     1381 aacaagcatt cctttaaaaa agattctttt atataagtga aaaaagcttt tttccaccaa
     1441 ttaaataaat ctctcatctc aatagtttat ccattatttt atcttttttt gcgtatacct
     1501 actaaatatt accgtcttct tctcgttctt ctactttgtt cgagagaatc tgtactagaa
     1561 aaagtagttg tatgtctacc ttttctgcct atccgagact ttcttttcat atatgggata
     1621 taaatgatta gatacagtac agcaaaaaat aaagcaaata cgcaagcttc ccaactacct
     1681 aacgccaatt caaccatcaa ggtagcaacc ccaacggatg ctgcatacca gataaaaatc
     1741 agaaacgttt ctttttttct gaccatgcga aacatgataa atagactaat aagcaatagt
     1801 ggcaacacaa atatccaatt gcttccttcc atcagccact tgagtatatc aagcagcgac
     1861 tgtgcaacag cgactaattt tcccatagtg aacacacacc tttttttata attaaaaaat
     1921 tcgccttttt attctactat gaatcttctg gcttggcttt catttctctt atgtaatcat
     1981 tttcttttat acaacttaaa aaaaaattaa accattagca taatattttc tgaatctatt
     2041 cacgaaagtc tttttctatc tttcgttttc cacgactcaa ctgatctaga gtcaaaaaac
     2101 taaaacgaca gttgcgcaaa tgacgcacaa gctgccgctt taataacaaa tagcctttca
     2161 ttctcagcag gccacttatt tccagttaac tttctgttct tagcttttta ctcaactgga
     2221 taatatatgc acttaatgaa gtcccaattt ccgaatgttg aagtccatat tcaatatttg
     2281 tcttcatata tcctaactta tcgcctacgt cgtatctttt tcctttaaac tctctcgcat
     2341 agaccgcctc tgtctggtta agagtctgaa gtgcatccgt tagctgtatc tcgtttcctg
     2401 ctcctggttc ttgagtttct aatatctcaa atattttagg agtcagtaga taacgtccga
     2461 taatggctaa attacttggt gcttttccca tttcaggttt ttctacaaac ccttttactc
     2521 tgcaacgacc cttttcgcta tctgttttag gatcaataat tccgtattta gatgtttcat
     2581 tttctggtac acgcatgacg gcaacagcgg aagcttgtgt ttcttcataa tccatcatca
     2641 attgttgagt caatgggata tcatctttca tcaaatcatc ccccagcatg acgacaaacg
     2701 gttcatttcc tataaatgct tttgcttgaa gcacggcatc ccccagtcct cttggatgag
     2761 attgacggat gaagtgaaga ttgacttgag tcgtttcttc aactaaagcg agtaattctg
     2821 ttttttcctt ttcccgcaaa ttgttttcca attcgatatt tgcatcaaag tgatcttcaa
     2881 tgggtctttt ttcttttccc gttacgatca agatgtcttc gattcccgaa tccaatgctt
     2941 cttccacaat aaactggatc gttggtttat ccacgattgg aagcatttct tttgccatgg
     3001 cttttgttgc cggcaaaaaa cgtgttccta gcccagcggc aggaatgact gcttttttga
     3061 ccttcacttg ct
//
LOCUS       DACDKM010000174         3024 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20834_2016
            SAMN12640370-rid9891663.denovo.176, whole genome shotgun sequence.
ACCESSION   DACDKM010000174 DACDKM010000000
VERSION     DACDKM010000174.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640370
            Sequence Read Archive: SRR10040891
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3024)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3024)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742835.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742835.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:04:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,012
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 29 of 75
            Pseudo Genes (incomplete)         :: 33 of 75
            Pseudo Genes (internal stop)      :: 19 of 75
            Pseudo Genes (multiple problems)  :: 5 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3024
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640370-rid9891663.denovo.176"
                     /isolate="E20834_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            638..>3024
                     /gene="esp"
                     /locus_tag="IV334_002718"
     CDS             638..>3024
                     /gene="esp"
                     /locus_tag="IV334_002718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="repetitive surface protein Esp"
                     /protein_id="HAP8944723.1"
                     /translation="MVSKNNKRVFLEKTKKRVLKYSIKKLSVGVASVLIGVGLVLGTT
                     ELVKAQDEISPSSPLETAISSVQVGDKVASGNTFQENPGYTKNYNFSDLQFSPQELTG
                     DTLKGNTIGFEVYGKHNIAASTKNWEIRLQLDERLAKYVEKIQVDPKKGIGSSRRTFV
                     RINDSLGRPTNIWKVNYIRASDGLFAGAETTDTQTAPNGVITFEKSLDEIFKEIGIDN
                     LKTDRLMYRIYLVSHQDDDKIVPGIDSTGYFLTDSDDFYNSLDVSENNPDQFKHGSVS
                     AKYEKPNTQTKDGSGSTGANGAIILDHKLTKNYNFSYSSSAKGTPWYANYKIDERLVP
                     YVAGIQMHMVQADKVTYDVSFESGTKVADLAIERREGHENYGIGSITNNDLTKLIDFA
                     NASPRPVVIRYVLQLTKPLDEILEDMKATAQVEENKPFGEDFIFDSWLSDTNKKLIQN
                     TYGTGYYYLQDIDGDGNPDDKEESGDTNPYIGKPELEEVYDVDTTVKGKVFIHELAGT
                     GHKAQLVDKEGTVLAEKTIAPNEKDGAPISDTVEFEFTGVDSSKLIAKDELKIQIVSP
                     GFDKPEEGSTVIKESPKAVDKQTVVVGFKPDAKESIRNNKNLPEDAEYSWKTEPDTSN
                     VTDSTKGIVTVKIGNRTFDVDVEFAVKASQAMENDATYVPITTTPETTVQSGKPTFDK
                     PDVPLAKDAFSISDVYNKYFGNASVDANTGIVTFTPAKGVGESEPITGTIPIKIVYQD
                     GSVGTTDLAVTVSKDIYENPGENIPAGYHKVTFTAGEGTSIESGTTVFAVKDGVS"
BASE COUNT         1102 a          457 c          595 g          870 t
ORIGIN      
        1 tatttgagtg aaagcaatta attattcatg tagagtacca gacactttaa atatggacaa
       61 ttcaattata aagaaaaaga atcaataatt taatgaggct gtgacaaatg tcattttttg
      121 tttcgctaaa aacgagtgaa aaaatgttga taactccaat cattcgttta agattctagg
      181 aatcattcag aaatgctgtg aaaccgatac tttataatta taaagaaggt gatttcaaat
      241 tccgaagaag gaatttgaag tcaccttttt acttatgtca cagtctcatt cagtaatgcg
      301 ggataaggct ccttattccg ttatttgaat actctgaagt ttaatctaag aaagggtaat
      361 attttgaaaa catgcttcat cgcatagaaa tgaacttaat ttcagaaaga atacatacat
      421 atctttttat atgttttcag ctgctgaatg gttaaaaagc acaaatatcc gaaaaaaaca
      481 tgaagaaatg tacttttttt gttgtaatgt tcatgaaatt ctctaagtaa attagttaac
      541 ataaggttag taagattcct attctgtgaa taatacgtat cagatttatc ttttattctt
      601 actaggaaag taattattaa gggagttgat ttgaataatg gttagcaaga ataataagag
      661 agtatttcta gaaaaaacaa agaaacgagt attgaagtac agtatcaaaa aattaagtgt
      721 tggggttgca tctgttttga ttggtgttgg tcttgtcttg ggaacaactg aactagttaa
      781 agcacaagat gaaatttctc caagcagccc acttgaaaca gcgataagct ccgtacaagt
      841 aggtgacaaa gtagcttcgg ggaatacttt tcaggagaat cctggttata cgaaaaatta
      901 taacttttcg gatttacaat ttagtcctca agaactaact ggagacacac taaaaggcaa
      961 tacaattgga tttgaagttt acggaaaaca taatattgca gcatcaacta aaaactggga
     1021 aatccgtctt caattagatg aacgattagc taaatatgta gaaaaaattc aggtcgatcc
     1081 aaagaaagga attggatctt ctagacggac ctttgtaaga attaatgatt cgctgggtag
     1141 acctacaaat atttggaagg tcaattatat tcgtgcaagt gatggattat ttgctggggc
     1201 agaaacaacc gatacgcaaa ccgctcctaa tggggtaatt acgtttgaaa agagcctaga
     1261 tgaaattttt aaagagatag gcatagacaa tctcaagacc gatcgtttaa tgtatcgaat
     1321 ctacttggta agtcatcaag acgatgataa aattgtaccg ggtattgata gtacgggcta
     1381 ctttttaaca gattcagatg atttctataa tagcttagat gtatctgaaa ataatcctga
     1441 ccagtttaaa catggatcag taagtgcaaa atatgagaaa cctaatactc aaacaaaaga
     1501 tggttcagga tccacaggtg ctaatggagc tataatctta gatcataagt taacgaaaaa
     1561 ttataatttc tcatattcat cttccgcaaa gggcacgcct tggtatgcta attacaaaat
     1621 tgatgaacga ttagttccat atgtagcggg tatacagatg cacatggttc aggctgacaa
     1681 agtaacatat gatgtttcgt ttgaatccgg aacaaaagta gctgatttag caatcgagcg
     1741 acgggaaggt catgaaaatt atggcatagg ttcaataact aataatgatt taactaaact
     1801 tatcgatttt gctaatgcaa gtccacgtcc agtcgttatt cgatatgttt tacaattgac
     1861 taagccatta gatgaaatct tagaagatat gaaagcaaca gcacaagttg aagaaaataa
     1921 accatttggt gaagatttca tctttgattc ttggttgtcg gatacgaata aaaaattaat
     1981 ccagaacact tatggaacag gttattatta tttgcaagat attgatggtg atggaaaccc
     2041 tgacgataaa gaagagagcg gagacacgaa tccatatatc gggaaacctg aattagaaga
     2101 agtatatgat gttgacacaa cagttaaggg gaaagtattc atccacgagt tagcgggaac
     2161 aggtcacaaa gcccaacttg ttgataaaga aggtactgta ttagcagaaa aaactatcgc
     2221 tccaaatgaa aaagatgggg ctccaatttc agatactgta gaatttgaat ttacgggtgt
     2281 agattcaagt aaattaatcg cgaaagatga attaaaaatc caaatcgttt ctccaggttt
     2341 tgataaacca gaagaaggtt caaccgttat taaggaatca ccaaaagcgg ttgataaaca
     2401 aaccgtggta gttggattta aaccagatgc taaagaatca attcggaata ataaaaactt
     2461 acctgaagat gcagagtatt catggaaaac agagcctgat acttctaacg ttactgatag
     2521 tacgaaaggt attgtaactg ttaagatcgg aaatcgaact ttcgacgtgg atgtagagtt
     2581 tgctgtaaaa gcttctcaag ctatggaaaa tgatgcaaca tacgtaccta taacaacaac
     2641 tccagaaacg acagttcaaa gtggaaaacc tacatttgat aaaccagatg ttcctttagc
     2701 taaagatgcc ttttcaattt cagatgtcta taataagtac ttcggtaatg caagtgttga
     2761 tgcaaacact ggtattgtta cattcactcc agctaaaggt gtaggagaat cggagccgat
     2821 tactggaaca attcctatta aaattgttta ccaagatggt tctgtaggca cgaccgattt
     2881 agcagtaact gtaagtaaag atatttatga aaatccagga gaaaacattc ctgcaggcta
     2941 ccacaaagta accttcaccg caggggaagg aacaagtatt gaaagtggaa caacagtctt
     3001 tgcagtgaaa gacggcgtaa gctt
//
LOCUS       DACDKM010000175         2891 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20834_2016
            SAMN12640370-rid9891663.denovo.178, whole genome shotgun sequence.
ACCESSION   DACDKM010000175 DACDKM010000000
VERSION     DACDKM010000175.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640370
            Sequence Read Archive: SRR10040891
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2891)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2891)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742835.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742835.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:04:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,012
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 29 of 75
            Pseudo Genes (incomplete)         :: 33 of 75
            Pseudo Genes (internal stop)      :: 19 of 75
            Pseudo Genes (multiple problems)  :: 5 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2891
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640370-rid9891663.denovo.178"
                     /isolate="E20834_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(920..2260)
                     /gene="glnA"
                     /locus_tag="IV334_002719"
     CDS             complement(920..2260)
                     /gene="glnA"
                     /locus_tag="IV334_002719"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HAP8944724.1"
                     /translation="MVKKQITGEEIKRIIEEENVRFLRLMFTDILGTIKNVEVPVSQI
                     DKVLENKMMFDGSSIEGFVRIEESDMYLYPDLSTWMIFPWESAHGKVARLICDIYNPD
                     GTPFAGDPRGNLKRALKDMRDLGFTSFNLGPEPEFFLFKLDEDGGITTTLNDKGGYFD
                     FAPTDLGENCRRDIVLELESLGFEVEASHHEVAPGQHEIDFKYADVVDACDNIQTFKL
                     VVKTIARKHGLHATFMPKPLFGINGSGMHCNMSLFNDEGNVFYDKDGELELSETAYHF
                     LGGLLKHARAYTAVCNPTVNSYKRLVPGYEAPVYVAWSGRNRSPLVRVPESRGLSTRL
                     ELRSVDPSANPYLAMAVLLQAGLDGIRNQITPPAAVDRNIYVMDEEEREAAHIQDLPS
                     TIHNAIKELRKDNVMIDALGQHIFSNFVEAKRLEWAAFRQTVSEWEREQYLELY"
     gene            complement(2288..2680)
                     /locus_tag="IV334_002720"
     CDS             complement(2288..2680)
                     /locus_tag="IV334_002720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HAP8944725.1"
                     /translation="MGEKELRRSMSVFPIGTVMKLTDLSARQIRYYEEQDLVHPERSE
                     GNRRMYSLNDIDMLLEIKDYLSEGLNMAGIKRVYEMAQAKKQEAKNKKPLTDHDVRQI
                     FRDEILAQGGLSKTGHYQSQGLQRQFFD"
BASE COUNT          879 a          617 c          464 g          931 t
ORIGIN      
        1 cttgatataa acatagctaa cgttaaacct aagcaaatca gaaaatcgtt actgaaaaat
       61 tttaagtgaa ataatagata aaaaaataaa acaaacatta tgatagataa tatcccacct
      121 aacaaagaac tactttttcc tacttgcagc aacagtttat atagcttatc gtagaaacaa
      181 tctttttctt tcacccttat ttacctcctt tacgtaaaaa acgtagtcca cagttctcgg
      241 atttttggca tatcttttct attaaataat atcaaaaaca aaacaattcc actaattttt
      301 tcaaagatga cctttttttc aacaaaagtg ccttgtaggg attcattgaa ggtaacgaat
      361 cacataagta aaaaataagg agatgcgaca aaagtcgtgc ctccttattt ctaaataaac
      421 ggtgttctcc tgccaactcc tcggtcacaa gtcacaatat ccatcaaaca tgagaagctg
      481 tttttgaaat tatttcttgt gattcggagt taaatggtag gctcacaacc tctggataaa
      541 cggtattcaa aagccagttt ctcggtatta agccaaattt atgcaaaaat gtagaaagcc
      601 attttcacaa aatttttctt aatactcgaa actaaacgtc tttttcaaaa cctcttggta
      661 aacggtgttc aaaagcttgc atctgcggta ataagttcag ccaattcaaa aacaagaaaa
      721 gctgtttttg cttggctatt cttattactt gttgcagggc agctttttca caacctctcc
      781 actaaacggt gttcaatcag ctgaccctcg gtattagctc aaaaaacggc aaaatatgag
      841 gaactatttt tccgttagtt tctctaatac tcaggtcaaa acgctgattt cacagcctct
      901 aattcacggt attcctgttt taataaagtt ctagatattg ttctctttcc cactcggaaa
      961 cagtttgtct gaatgccgcc cattcgagac gttttgcttc aacaaaattt gagaagatat
     1021 gttgtcctaa tgcgtcaatc attacattat ccttacgcaa ttctttgata gcattatgga
     1081 tagttgaagg taagtcctgg atatgtgcag cttctctttc ttcctcatcc attacataaa
     1141 tgttacggtc aacagctgct ggcggtgtga tctgattacg gataccgtct agtcctgctt
     1201 gcaacaatac tgccattgcc aagtatgggt ttgcagatgg atcaactgac cgcaattcca
     1261 aacgtgtgga taaaccgcga gattctggta cacgtactaa tggcgaacga ttacggccac
     1321 tccatgcgac ataaacaggc gcttcataac ctggaacgag acgtttataa gaattgactg
     1381 ttggattgca aacggctgtg taggcacgag catgtttcag caaaccacct aagaaatgat
     1441 aagctgtttc acttagttcc aattcaccgt ctttatcata gaaaacattt ccttcatcat
     1501 tgaacaaaga catattgcaa tgcataccag aaccattgat cccaaataac ggtttcggca
     1561 tgaaagtcgc gtgaagtcca tgcttgcgtg caatcgtttt gacaacgagc ttgaaggttt
     1621 ggatattatc acaagcatct accacatctg catatttaaa gtcaatttca tgctgtcctg
     1681 gcgctacttc atgatgtgaa gcttccactt caaagcctag actttctaat tccaacacga
     1741 tgtcacggcg gcaattttca cctaaatctg ttggtgcaaa atcaaaataa ccacctttat
     1801 cattcaaggt cgttgtgatc ccaccgtctt cgtctaattt aaataagaag aattctggtt
     1861 caggtcctaa attgaaagag gtaaaaccaa ggtcgcgcat atctttcaat gctcgtttca
     1921 aattcccgcg aggatcgccg gcaaatggtg tcccatccgg attatagata tcacaaatta
     1981 aacgagcaac ttttccatga gcactttccc acgggaagat catccaagtg gagagatctg
     2041 gatacaaata catatcgctt tcttcaattc gaacaaatcc ttcaatcgaa gaaccgtcaa
     2101 acatcatttt attttctaat actttgtcga tctggctcac tggaacttct acatttttga
     2161 tcgtacctaa gatgtctgtg aacatcaatc gtaaaaatcg tacgttttct tcctcaatga
     2221 ttcgtttgat ttcttcacct gttatctgtt tctttaccat ggtatccctc ttcctttttt
     2281 aatttattca gtcaaagaat tgtctttgca atccttgcga ctgataatga cctgttttgc
     2341 ttaacccacc ttgcgccaaa atctcatcac gaaaaatttg tcgaacgtca tgatctgtca
     2401 acggcttctt gttttttgct tcttgttttt tcgcctgtgc catttcgtat acacgtttga
     2461 tcccagccat attcaatccc tctgaaagat agtctttaat ctcaagcaac atatcaatat
     2521 cattcagcga atacatacgt cggtttcctt cgcttcgttc cggatgaact aaatcttgtt
     2581 cttcataata gcgaatttgc cgagcagaca aatcagtcag cttcataact gtaccaattg
     2641 gaaatacgga catagatcgg cgcaattcct tttctcccat cagacgtccc ctttcgtttt
     2701 ataagttggc taaagtgtac caattaaaaa aaatattgtc aactgcatat gttaggtttt
     2761 ctaacatgag atctctattt tttataaaat aacaaaaaaa taagaacaca aaaaaggtat
     2821 ctagttgatt ttcgtaccag atacctttta ttttttatta tactgtacta tcctttatta
     2881 gtgtttttat t
//
LOCUS       DACDKM010000176         2888 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20834_2016
            SAMN12640370-rid9891663.denovo.179, whole genome shotgun sequence.
ACCESSION   DACDKM010000176 DACDKM010000000
VERSION     DACDKM010000176.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640370
            Sequence Read Archive: SRR10040891
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2888)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2888)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742835.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742835.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:04:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,012
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 29 of 75
            Pseudo Genes (incomplete)         :: 33 of 75
            Pseudo Genes (internal stop)      :: 19 of 75
            Pseudo Genes (multiple problems)  :: 5 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2888
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640370-rid9891663.denovo.179"
                     /isolate="E20834_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(<1..242)
                     /locus_tag="IV334_002721"
     CDS             complement(<1..242)
                     /locus_tag="IV334_002721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8944726.1"
                     /translation="MFEEFIDINERQVYQFLNYCYERDEKLYVVKDIALDLNYTLAKM
                     NSVIQQAESFCERYPEYKLSFLSENKMIKVEFSSQF"
     gene            complement(540..1514)
                     /gene="sgrA"
                     /locus_tag="IV334_002722"
     CDS             complement(540..1514)
                     /gene="sgrA"
                     /locus_tag="IV334_002722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG-anchored fibrinogen/nidogen-binding
                     adhesin SgrA"
                     /protein_id="HAP8944727.1"
                     /translation="MKKTATVLFVILGLSIIAAMIGISTSANERANENLSFTVKTDRI
                     VYDMTQQVITIPVKPNKSVNASDVHAVLTYGWDGNGSSEKVIGEVYLKDVQWTAGIEY
                     TIMISAELSIDEIKSKDKVDLIVFYDGQMTITENLKPSSWTVVGPPSSTDSTSSESST
                     ENTSGESSTESTSSESSTENTSSESSTESTSSESSTGSTSSESSTESTSSESSTGSMS
                     SESSTGSTSSESSTGKVNNELDLVPIIPTKEQKRNAGSNLKSVLKQPDFVSRGIDHQE
                     KAKETDNRKSDLPETGSRTLNKLITWMGVLLILIVGASYFRSLFHRVK"
     gene            complement(1710..2549)
                     /locus_tag="IV334_002723"
     CDS             complement(1710..2549)
                     /locus_tag="IV334_002723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HAP8944728.1"
                     /translation="MIRIIRLIGSVLIVLVGFVLGILVNNALADMFIGNSEWIGAIAG
                     TVGHLAVFLLALFLASKVEGKRIEDYGVSVRGKDWGYLAGGLVVGIGVFLLITSPLYL
                     TGAYRLDSGNANIVPLITSFILFIAVGASEELLFRGFFQHQMLRFGPLIAMIGSAALF
                     ALLHGLNPNMTFLAVFNIFLAGCFFSALIYSTGSLFTAIGAHITWNWTQGAILGIPVS
                     GTQEQGFFSTLIQSQNTIITGGNFGAEASISTTLFLLVLIVGLLLYAYKTKRMKKYTA
                     VYQ"
     gene            2734..>2888
                     /locus_tag="IV334_002724"
     CDS             2734..>2888
                     /locus_tag="IV334_002724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rotamase"
                     /protein_id="HAP8944729.1"
                     /translation="MGNFLKKQLLIMCLSACAILLFSGCSKSNETIATFKGGTLTVQD
                     FYDNPKV"
BASE COUNT          878 a          633 c          417 g          960 t
ORIGIN      
        1 aaaaactgag aagaaaactc gacttttatc atcttgtttt ctgaaagaaa agataattta
       61 tattctggat acctttcgca aaacgattct gcttgttgaa tcaccgaatt catcttagcc
      121 agggtgtagt ttagatctaa agcgatatcc ttcaccacat ataatttctc gtctctttca
      181 taacaatagt tcaaaaattg atatacttgc ctttcattga tatcaataaa ttcttcaaac
      241 aaagtacata ctccttttta tctattattt agacttattc cgacagctac atgaataacg
      301 tacaaataga ttcgttactg atttttcaga gatactacca tgcgcagtgt gcattgtcaa
      361 ttattcttgc tcttattttc catttgtgtt cctgttattt cctgtttctc aaatatgtta
      421 gtaaacaaaa atagatctgg caaagctaca acttcgccag gcaatacagc actctcgaat
      481 gaaatattca ctcaaaaatc tatcgtttta ttcaatttta gatctgttgc ttttattctt
      541 tatttcactc gatggaagag agaacgaaaa tagcttgctc ctacaatcaa aatgagtaac
      601 acacccatcc atgtaattag cttgttcaag gttctactac cagtttctgg taaatcgctt
      661 tttctattat ctgtttcttt cgctttttct tgatgatcaa ttccacgaga aacaaaatca
      721 ggctgtttta gcacactttt taggttgctc cctgcatttc tcttttgttc cttagttggt
      781 atgataggta ccaaatcaag ttcattattt acttttcctg tgctgctttc gctgctcgtg
      841 cttcctgtgc tactctcact actcatgctt cctgtgctac tttcactact cgtactctcc
      901 gtactgcttt cgctgctcgt gcttcctgtg ctactttcac tactcgtact ctccgtactg
      961 ctttcgctgc tcgtgttttc tgtgctactt tcactgctcg tactctctgt actgctttca
     1021 ccgctcgtgt tttctgtact gctttcacta cttgtgctat ccgtactgct tggcggtcca
     1081 acaactgtcc aactgcttgg tttcaaattc tccgtgatcg tcatctgtcc atcataaaaa
     1141 acaatcaaat caactttatc tttactcttg atctcatcaa tcgatagttc tgcagaaatc
     1201 attattgtat actcaatacc agcggtccat tgtacgtctt ttaaataaac ttccccaata
     1261 actttttctg aacttccatt accatcccaa ccatatgtta gaacagcatg tacatcacta
     1321 gcattcacgc ttttgtttgg tttgactgga atagtaatga cttgttgtgt catgtcataa
     1381 acaatccgat cagttttcac agtaaatgaa agattctcat ttgcccgttc atttgcagaa
     1441 gtactgattc caatcattgc tgcaattata ctcagcccaa gaataacaaa taatacggtc
     1501 gctgttttct tcatttctat cactctccat ataattacct tctatttgat aaaggaatta
     1561 tataaaatga aaatgatgag aacacgacag tcagttgtga aaatctaaca aaagatgtcg
     1621 tgtattcgct tgaatttgtt tactttttcg tttaatcaat gacagactta aaaaagcaag
     1681 accgatatca gccttgcttt cttcatcttt tattgataaa cagcagtgta tttcttcatc
     1741 cgctttgttt tatatgcata gagtaacaaa ccaacaatca agactaacaa gaataatgta
     1801 gttgatatag atgcttcagc cccaaagttt cccccagtaa ttattgtatt ttgcgattgg
     1861 atcagagtgg agaaaaaacc ttgttcttgc gttccgctga ctggtatacc caaaatagct
     1921 ccttgtgtcc aattccaagt aatatgagca ccaatagccg taaataaact tcctgtactg
     1981 tagataagtg cgctgaaaaa gcaacctgct aaaaagatat tgaaaactgc cagaaatgtc
     2041 atgttaggat tcaatccgtg caaaagagca aataatgcag cagatccaat cattgctatt
     2101 aacggaccaa atctaagcat ctgatgctga aaaaagccac ggaataaaag ctcttctgat
     2161 gcccccactg caataaataa aataaaactc gttatcagcg gcacaatgtt tgcgtttcct
     2221 gaatctaacc gatacgcccc cgtcaaatat aatggactag taataagcag aaacactcca
     2281 attccaacaa cgagacctcc cgctaaatac ccccagtctt ttcctcgaac agaaacacca
     2341 taatcttcta ttctttttcc ttcgacctta ctagcaagaa ataaagctag taaaaaaaca
     2401 gctaaatgtc cgaccgtacc ggcaattgct ccaatccatt cactatttcc aataaacata
     2461 tccgcaagtg cattattgac aagaattcct aaaacaaaac caactaagac tattaaaaca
     2521 gatccaatca accgaataat cctgatcatc tttcatccct cctttatatt aagtttaaca
     2581 acccttttct ttcttgtctc gtgaaaacta tgtaaatgtt ctccatcata gagagaatag
     2641 atttttacga actaagaata tctacagcaa ccttacgaaa atgaaagact gtctccctaa
     2701 ttcttctgtt agactgtttt taataaggaa aggatgggaa attttttgaa aaaacaacta
     2761 ctaattatgt gcctttcagc atgtgcaatt ttactattct caggttgtag caagtccaat
     2821 gaaacgattg caacgtttaa aggtggaaca cttacagttc aggattttta tgataaccca
     2881 aaagttaa
//
LOCUS       DACDKM010000177         2879 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20834_2016
            SAMN12640370-rid9891663.denovo.180, whole genome shotgun sequence.
ACCESSION   DACDKM010000177 DACDKM010000000
VERSION     DACDKM010000177.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640370
            Sequence Read Archive: SRR10040891
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2879)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2879)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742835.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742835.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:04:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,012
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 29 of 75
            Pseudo Genes (incomplete)         :: 33 of 75
            Pseudo Genes (internal stop)      :: 19 of 75
            Pseudo Genes (multiple problems)  :: 5 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2879
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640370-rid9891663.denovo.180"
                     /isolate="E20834_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(32..1456)
                     /locus_tag="IV334_002725"
     CDS             complement(32..1456)
                     /locus_tag="IV334_002725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HAP8944730.1"
                     /translation="MWEKVEKKQGPIYRQIMEQIMREIENGRINPGSQLESERKLGQL
                     FGVNRSTVVHALEELRELGVLTSKRGSGWFVNQTEWGKFAVPRIDWRQMISPRYEQTD
                     WYEQKIREAKQIQKTSFLDLYASEMPDELLPNFEFPSYSLEEILAEEKEMDLLGYTPL
                     RNKIKRYLESKFELAFSPNQLLVTGGGQQAIFLILQTILSAGEGIAVESPSFFYRLPL
                     FKASGTRLFGIPMDEEGIDLEKLKESIHKNKLKAVLINPNFQNPTGKVMSQKRREKLV
                     ELCRQYRLPIIEDDVFTDLSFLNQEEVLPIRTIDPDNVLYVGSLSRVLGKTTKIGWVI
                     GPETFISQLAQAQEMMEFSMNILTQITVANVFNEAIDLKMQDVRKKLKEKSRLISAWG
                     DSQSFFKLHSILGGYYAWITWDGKKLDPELAEKVVDMGLGIAPSLLFGQDINGIRINY
                     SRLDENQIPLFYSKMKILEKWLTE"
     gene            complement(1720..2697)
                     /gene="guaC"
                     /locus_tag="IV334_002726"
     CDS             complement(1720..2697)
                     /gene="guaC"
                     /locus_tag="IV334_002726"
                     /EC_number="1.7.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP reductase"
                     /protein_id="HAP8944731.1"
                     /translation="MKVFDYEDIQLIPNKCIVNSRSECDTTVTLGKHTFKMPVVPANM
                     QTIIDETIAEFLAENGYFYIMHRFDEAARIPFIKKMKKRGLITSISVGVKKEEYSFIE
                     KLAEESLNPDYITIDIAHGHANSVIDMIQHIKKYLPETFVIAGNVGTPEAVRELENAG
                     ADATKVGIGPGKVCITKIKTGFGTGGWQLAALRWCAKAARKPIIADGGIRTHGDIAKS
                     VRFGATMVMIGSLFAGHEESPGETKVENGIVYKEYFGSASEFQKGEKRNVEGKKIWIQ
                     HKGSLKDTLVEMQQDLQSSISYAGGRDLEAIRKVDYVIVKNSIFNGDTI"
BASE COUNT          824 a          619 c          451 g          985 t
ORIGIN      
        1 ttatattctt taatctattt actttgtgaa attattctgt taaccacttt tctagaattt
       61 tcatctttga ataaaacaac ggtatttggt tttcatccag cctactgtaa ttgattctga
      121 ttccatttat atcttgccca aaaagtagac ttggtgcaat tccaagaccc atatctacta
      181 ctttttctgc tagttcgggg tctagttttt tcccatccca agtaatccag gcatagtaac
      241 cacctaaaat tgaatgaagc ttgaaaaacg attgtgaatc tccccaagcg cttatcagtc
      301 ggcttttttc tttcaacttt tttcttacat cttgcatttt caaatcaatt gcttcattaa
      361 atacattagc cactgtgatt tgtgtaagga tattcatcga aaactccatc atttcttgcg
      421 cttgagctag ttgcgagata aaagtctcag gtcctatcac ccaaccgatt ttcgttgttt
      481 ttcctaaaac cctagataac gaaccgacgt ataacacatt atccggatca atcgttcgta
      541 taggaaggac ttcttcttgg tttagaaatg aaagatccgt aaatacatcg tcttcaataa
      601 ttggaagccg atactgtcga cacaattcta ctagcttttc tcgtcgtttt tgactcatca
      661 cttttcctgt agggttttga aaattcgggt tgataagcac tgctttcaat ttatttttat
      721 ggatcgattc tttcagtttt tctaagtcga tcccttcttc atccatcggt atcccaaata
      781 aacgcgttcc tgaagctttg aaaagaggta atcggtaaaa aaaagaaggt gactctacag
      841 ctatcccctc tccagctgaa agaatcgttt gtaaaatcaa aaagattgct tgctgaccgc
      901 caccagtcac aagaagctgg ttgggggaaa atgccaattc aaatttactt tccaaatacc
      961 gtttgatttt gttacgtaac ggagtatagc ctaaaagatc catttctttt tcttccgcta
     1021 agatttcttc aagagaataa gaaggaaact caaaattcgg caaaagctca tctggcatct
     1081 cgctagcgta taaatcaaga aacgatgttt tttggatctg ctttgcttct cttatttttt
     1141 gctcatacca gtccgtctgc tcataacgag gagagatcat ttgccgccaa tcgattcttg
     1201 gaacagcgaa tttcccccat tcagtctgat taacaaacca tccgctgcca cgtttgctcg
     1261 ttagtacccc aagctcccta agctcttcca atgcatggac aacggtggaa cgattcacac
     1321 caaaaagttg ccctagtttt cgttctgatt caagttgact tccaggattg atccttccgt
     1381 tttcgatttc tctcataatc tgttccataa tttgacgata aatcggtcct tgttttttct
     1441 ctactttttc ccacataagc ttctcttttc tattgtctat tttttctttt gattttaccg
     1501 aatttacgag aaaaaagaaa aacttttttt gcaaaacata agggactgtg acaaaaatct
     1561 tatcacagtc ccttattctg aataatgatg ttcaaaagct tgtatctgcg ataataattc
     1621 cagccaaaca aaacatataa agaactattt ttgtttggct gttcttatta cttaatgcag
     1681 gacaactttt tcacaacatc ctaatatttt atttattttt tagattgtgt ctccattaaa
     1741 aatcgaattt ttcacgatga catagtcaac ttttcgaatg gcttccagat ctcttcctcc
     1801 agcataagaa atagatgatt gaaggtcttg ttgcatttcc actagtgtat ctttcaaaga
     1861 tcctttgtgt tggatccaga ttttctttcc ttcaacattt cgtttttcgc ctttttggaa
     1921 ttctgaagca gaaccaaagt attctttata cacgattcca ttttcgactt ttgtttcgcc
     1981 aggagattcc tcgtgaccgg caaacaatga accaatcatg accattgtcg caccaaaacg
     2041 gacagatttt gcaatatcgc catgtgttct gatcccaccg tcagcgatga tcggcttgcg
     2101 agctgctttc gcacaccatc taagagctgc cagttgccag cctccagtcc caaagcctgt
     2161 cttgattttt gtaatacata cttttcctgg accaatcccg acttttgttg catctgcgcc
     2221 cgcattttcc aattcacgga cggcttccgg tgtaccaaca ttaccggcaa tcacaaaagt
     2281 ttcaggcaaa tattttttaa tatgctgaat catatcaata actgagttag catgtccatg
     2341 cgcgatgtcg atagtgatat aatcaggatt tagcgattct tctgccaact tctctataaa
     2401 ggaatattcc tctttcttga cgccaacact gatcgaggta atcaatccac gctttttcat
     2461 ttttttgata aacggaattc gagctgcttc atcaaaccga tgcatgatat agaaataacc
     2521 attttccgct aagaattctg cgattgtttc atcaataatc gtctgcatgt tggcaggcac
     2581 tactggcatt ttgaatgtat gtttacctaa tgtgaccgtt gtatcacatt cagaacggct
     2641 atttacgata catttattag gaattaattg aatatcttcg taatcaaata ctttcattac
     2701 attatcctcc tgatgttcac atccagcaaa acacgaacat tattaattta atcaaggctt
     2761 tttttagcgc cctatgtaat ttaactcaat taagcaaaaa aatcaagagc aaaaagagaa
     2821 tttatacgaa tggtaaaaaa acatatatta tttttagtct atacaaacga aacaatgaa
//
LOCUS       DACDKM010000178         2851 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20834_2016
            SAMN12640370-rid9891663.denovo.181, whole genome shotgun sequence.
ACCESSION   DACDKM010000178 DACDKM010000000
VERSION     DACDKM010000178.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640370
            Sequence Read Archive: SRR10040891
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2851)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2851)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742835.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742835.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:04:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,012
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 29 of 75
            Pseudo Genes (incomplete)         :: 33 of 75
            Pseudo Genes (internal stop)      :: 19 of 75
            Pseudo Genes (multiple problems)  :: 5 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2851
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640370-rid9891663.denovo.181"
                     /isolate="E20834_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            802..1068
                     /locus_tag="IV334_002727"
     CDS             802..1068
                     /locus_tag="IV334_002727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8944732.1"
                     /translation="MAKITKNEQENLVENYTKKPAYSNSRLDAHFVCTSNPKLSFDAM
                     TIVGNLNKNSAKKLSDFMSLDPQIRLWDILQTKFKAKALQDPLC"
     gene            1126..1638
                     /locus_tag="IV334_002728"
     CDS             1126..1638
                     /locus_tag="IV334_002728"
                     /inference="COORDINATES: protein motif:HMM:NF013677.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003321317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAP8944733.1"
                     /translation="MTNKNNERYSTELKATAIKKMMPPENRGIRQLSQTLGIPEATLY
                     SWRKKARIQGNTTPGTKKNVEKWSSEDKFLIVMESFSMNQVELAEYCRKKGLFKEQIE
                     AWKKTCLSANEQEENRTRELATELKEEKKQARQLEKELRHKEKALAEAAALLLLRKKA
                     HAIWGDQEEE"
     gene            1635..2672
                     /locus_tag="IV334_002729"
     CDS             1635..2672
                     /locus_tag="IV334_002729"
                     /inference="COORDINATES: protein motif:HMM:NF033516.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HAP8944734.1"
                     /translation="MISSSDRKQAVELIQEANRNGARLTYACNELNISVRTYERWTRE
                     GTIAHDQRPLAKRPVPKNKLTDQEREKIIETVSKKEFMNLPPSQIVPKLADCSIYIAS
                     ESSFYRVLREKNMQHHRGRSQVSQKRIPPSHLATKPNEVWTWDITWLKGPIKGLFYRL
                     YLIIDLFSRKMVGWEIWESEEARHAEKLIKQTVLKERIQGQPLVLHSDNGSPMKAATF
                     QVLLEKLGIQSSYSRPRVSNDNPYSEAAFRTLKYRPEYPNNGFKTIDDARKWTKEFVD
                     WYNDSHLHSGIQFVSPAACHEGRHLAILEKRKQVYEDAKKRHPERWSKGVRNWTPHQS
                     VALNPLKETES"
BASE COUNT         1030 a          502 c          569 g          750 t
ORIGIN      
        1 tttattgatg accacgaaag tgcatggcga tatttgatgt tgatacttta tgcagtaccc
       61 tatgtattct tcgctatcat tctgtggctc atagcattgt tttttagagt atttaatgac
      121 ctttaggtag atttatatat aaaaagggct aaatacgcaa aataccccct taaaatgcaa
      181 aataaatgca aataaggggg taactcctta tgaaaaaaca actaatagta ttataacacg
      241 tttttatgcc gagaaaactt attggttgga atgggctatg tgttagctaa cttgttagcg
      301 agttggttgg acttgaattg ggattaatcc caagaaagta ccaactcaac aacacataaa
      361 gccctgtagg ttccgaccaa taaggaaatt ggaataaagc aacgaaagga gttgagtaaa
      421 atgagtgaga aaacgaagaa aatcattcga gtatcattag ctttttcaca agtagtagtg
      481 caatcattga tagtttatta tacttaccag caatataaat tatctaaaga gaattaagtt
      541 ttgattatgt aataagtttt ggtactaaaa aagacttgat ctaattagac caagtctttt
      601 gttagtgtta tattaataac aaaataaaaa taagaagtcg ctcactcccc gaccaaagtt
      661 tgtgagcaaa ataaacagtc gatgtttgtt tcttaattgt atatcttgat attaaacgat
      721 ttaagattat tcttcaagat atatattcgc aaatgtaatt ggaaaagcga cttcttaaac
      781 aaaattaagg agtcgctttt tatggcaaaa ataacgaaaa atgagcaaga aaatttggtg
      841 gaaaattaca caaaaaaacc ggcgtactct aatagccggt tagacgcaca tttcgtgtgc
      901 acttctaatc cgaaattgag ctttgacgct atgacgatag tcggaaacct taataaaaac
      961 agcgctaaaa agctatccga ttttatgagt ttagatccac aaattagact ttgggatata
     1021 cttcaaacaa aatttaaagc taaagcacta caagaccccc tatgttaggc tagttgtcgt
     1081 ataggataat aagttataat aaagactata cggaggatga ttaccatgac aaataaaaac
     1141 aatgaacgtt actctaccga actgaaagcg acagccatca agaaaatgat gccacccgaa
     1201 aataggggga ttcgccaatt aagtcaaact ttgggcattc cagaagcgac tctttattct
     1261 tggcgtaaaa aagctagaat tcaagggaat actacacccg gaacgaaaaa aaatgtcgaa
     1321 aaatggtcaa gtgaagataa atttttaatt gtgatggaat cattctcaat gaaccaagta
     1381 gaacttgcgg aatattgccg aaagaagggc ttatttaaag aacaaattga ggcttggaag
     1441 aaaacgtgct tatctgcgaa tgaacaggaa gaaaatcgaa cgagagaact tgcaactgaa
     1501 ctaaaagaag agaaaaaaca agcgcgtcaa ttagagaaag aattacgtca taaggaaaaa
     1561 gcattagcgg aagccgctgc tctcttatta ctaagaaaaa aagcacacgc gatttggggg
     1621 gaccaagagg aagaatgatt tcatcttcag atcgcaaaca agctgttgaa cttattcaag
     1681 aagccaatcg caatggggcc cgtctcacct atgcctgtaa tgagctgaat attagcgttc
     1741 gaacctacga acgatggacg cgtgaaggta cgatagcgca tgatcaacgc cctttggcga
     1801 aacgtcctgt acccaaaaat aaactgacgg atcaagaacg cgaaaaaata atagaaaccg
     1861 tttcaaaaaa agaatttatg aatttgccac ctagccaaat tgtaccaaaa cttgcggatt
     1921 gctcgattta tattgcatca gagtctagct tctatcgtgt attgcgagaa aaaaatatgc
     1981 aacatcaccg tggccgaagt caagtttcac aaaaacgcat accgccaagt caccttgcga
     2041 cgaagccaaa tgaagtttgg acttgggata ttacttggct aaaaggtcca ataaaaggac
     2101 tcttttaccg gttatatctc atcattgatt tatttagccg taaaatggta ggctgggaga
     2161 tttgggaatc agaagaagct cgtcatgccg aaaaacttat taaacagacg gtgcttaagg
     2221 aacgtatcca aggacagccg ttggttttac actccgataa tggcagccct atgaaagccg
     2281 cgacttttca agtcttactc gaaaaattag gaattcaaag ttcttattca agaccacgag
     2341 tgagtaatga caatccttat tcggaagcgg ctttccgaac gttaaaatat cgaccagaat
     2401 atcccaataa cggctttaaa acaatagatg atgctcgaaa atggacgaaa gagtttgtcg
     2461 attggtataa cgattctcat ttgcatagtg ggattcaatt tgtgtctcct gcagcgtgtc
     2521 atgaaggacg acatctcgct attttagaga aacgaaagca ggtttacgag gacgctaaaa
     2581 agcgccatcc tgaacgctgg tcaaaaggtg ttcgaaattg gactccacat caatcggtgg
     2641 cgttgaatcc tttaaaagaa acagaaagtt aacaggagca taatctttca agattatcaa
     2701 ataaccctcg tcacccaata ggggaaaaag ttattcttcc cctattggat aacgaggcca
     2761 accgaccata gggaggttct gctttttgat aggtggaaaa taaaaatacg acaactatat
     2821 tgacaaacac cgaccatcgt ccagtaaaga g
//
LOCUS       DACDKM010000179         2843 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20834_2016
            SAMN12640370-rid9891663.denovo.182, whole genome shotgun sequence.
ACCESSION   DACDKM010000179 DACDKM010000000
VERSION     DACDKM010000179.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640370
            Sequence Read Archive: SRR10040891
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2843)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2843)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742835.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742835.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:04:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,012
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 29 of 75
            Pseudo Genes (incomplete)         :: 33 of 75
            Pseudo Genes (internal stop)      :: 19 of 75
            Pseudo Genes (multiple problems)  :: 5 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2843
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640370-rid9891663.denovo.182"
                     /isolate="E20834_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..952
                     /locus_tag="IV334_002730"
     CDS             <1..952
                     /locus_tag="IV334_002730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HAP8944735.1"
                     /translation="IVRQLLKYEPKAIRIYSRGEEKQFYMQQELSNHTNLRFLIGDIR
                     DSQRIDYACRDVDIIFHAAAMKHVPASEYNPMEAVKTNVMGTQNVIDAAIKNNVKHFV
                     GISTDKVVSPTNTMGATKLLSEKLIIAANQYKGKTETIFSCVRFGNVMGSSGSVIPLF
                     SKQIKNNKDITLTDVDMTRFMMSIPDAARLVLSAAMNSKFGETYVLKMPAINILTLAQ
                     SLIKIYNDTHDEKYRGEIKVVGIRPGEKLYEELMTTDEIERSYENDKMYIIPPHYHAT
                     DELYKDYEKIKITAYSSDATELMSGQEFIRIAKENNVVTW"
     gene            964..1968
                     /gene="neuB"
                     /locus_tag="IV334_002731"
     CDS             964..1968
                     /gene="neuB"
                     /locus_tag="IV334_002731"
                     /EC_number="2.5.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate synthase"
                     /protein_id="HAP8944736.1"
                     /translation="MKSEVYVIAEIGVNHNGSLDLALESIDKAKECGANAVKFQTFKT
                     NKLVSKKAPMAKYQKDNLKEDSSQFEMLKKLELSQSDFIRIKDYCIQNKIDFLSTPFD
                     EESADFLKQIGVQGFKIGSGDLTNLPFLRKIDMYGLPVLLSTGMADIHEVSEAVDCFI
                     DSPVTILHCTSNYPASSEDINLLAINTLKEMFGIPVGYSDHSLGYDVGICAVAMGAKV
                     IEKHFTLDKELPGPDHKASLDPQEFADFVKHIRNTEIFLGDGVKRAMPSELSTKEVSR
                     KSLVVTENLNVGDILTEKSLTIKRPGNGIAPKFLYQYIGKKVKRPISADSVLLEEDVE
                     "
     gene            1965..>2843
                     /gene="neuC"
                     /locus_tag="IV334_002732"
     CDS             1965..>2843
                     /gene="neuC"
                     /locus_tag="IV334_002732"
                     /EC_number="3.2.1.183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (hydrolyzing)"
                     /protein_id="HAP8944737.1"
                     /translation="MKKILIVSGTRADYGIYKPVAENLLQDNWDVSFVVSGMHLSHKY
                     GFTKELILADGFRISGEVQSLFLENNKGNMARSVALEINGFTNIFEQTSPDFVLLLGD
                     RGEMLAAAIACLYLGIKTAHIHGGEVSGTVDESIRHAITKIASVHFAATEKSKERLIK
                     MGEDSWRVFPVGAPRIDTILNNRLPSFRDIKEKYHLKFEKKKYVLQVFHPVVTELENI
                     EKQILALINSMEQQSQPIICILPNSDAGSEIIRKMYQKYLKTDIIYITNLEPEEYLTI
                     LKECKFMIGNSSSGIIE"
BASE COUNT         1055 a          368 c          547 g          873 t
ORIGIN      
        1 aattgttcgt cagcttttaa aatatgaacc aaaagctatc agaatttata gtagaggtga
       61 agaaaaacaa ttttatatgc aacaagagct ctcaaatcac actaatttga gatttttaat
      121 tggtgatatc agagactctc aaaggattga ttatgcatgt cgtgatgtgg atattatttt
      181 tcatgctgcc gcaatgaaac acgttccagc aagtgaatat aatcctatgg aagctgtaaa
      241 aacaaatgtg atgggaactc aaaatgtaat agatgcagcg atcaaaaata atgtaaaaca
      301 ttttgtaggt atcagtacag ataaagtagt gagtcctact aacaccatgg gtgcgacaaa
      361 gttattgagt gaaaaattaa taatagctgc caatcagtat aaaggaaaaa cagaaacaat
      421 attttcttgt gtaaggtttg gaaatgtcat ggggtcaagt ggatctgtta ttcctctttt
      481 tagtaaacaa ataaaaaaca ataaagatat tactttgaca gatgttgata tgacacgatt
      541 tatgatgtca ataccggatg ctgcaagatt agttctgtct gctgctatga attcaaaatt
      601 tggagaaaca tatgtcttaa aaatgccggc aatcaatatt ttaactttag ctcaatcatt
      661 aataaaaata tataatgata cacatgatga aaaatataga ggtgaaataa aagttgtagg
      721 tatcaggcca ggagaaaaat tatatgaaga gctaatgact acagacgaaa ttgaacgctc
      781 atatgaaaat gataaaatgt atatcattcc accacactat catgctactg acgaactgta
      841 caaagattat gaaaaaatta aaatcactgc atactcttca gatgcaacag aattaatgag
      901 tggacaggaa tttattagaa tcgcaaaaga gaataatgtc gtgacttggt aaggagtttt
      961 atgatgaaat cagaagtata tgtaattgca gaaattggtg taaaccataa tggaagtcta
     1021 gatttagctt tagaaagtat tgataaagct aaagaatgtg gagctaacgc ggttaaattt
     1081 caaacattta aaacgaataa gctagtttcc aaaaaggctc caatggcaaa atatcagaag
     1141 gataatctta aagaggacag ttctcaattt gaaatgctaa aaaaattaga attatctcaa
     1201 tctgatttta ttagaataaa agattattgt atacaaaata aaattgattt tttgtctact
     1261 ccttttgatg aagaaagtgc agactttcta aaacaaattg gtgtacaagg atttaaaatt
     1321 ggctcaggag atttaacaaa tttacctttt cttagaaaaa tagatatgta tgggttgcca
     1381 gttttattat ctacaggtat ggctgatatt catgaggtca gtgaggctgt agattgtttc
     1441 atagatagtc cagttactat tttacattgt acttcaaatt atccggctag ttctgaagat
     1501 attaatttat tggcgattaa cacattaaaa gaaatgttcg ggatacccgt tgggtactcc
     1561 gatcattcat tgggatatga tgttggtatt tgtgctgttg cgatgggtgc taaggtaatt
     1621 gagaaacatt ttaccttgga taaggaacta cctggtccag atcataaagc ttctttagat
     1681 cctcaggaat ttgcagattt tgtaaagcat attagaaaca ctgaaatatt tttaggtgat
     1741 ggtgttaagc gagcaatgcc ttctgaactt agtacaaaag aagtatcaag aaaaagccta
     1801 gtggtcactg agaatttaaa tgtgggagat attcttacag agaaatctct tacaataaaa
     1861 cgtcctggaa atggaatagc tcctaagttt ttatatcaat atattggcaa aaaagttaaa
     1921 agaccaataa gtgcagatag tgttctgctt gaggaagatg ttgaatgaaa aaaatactta
     1981 tagtaagtgg aacacgagct gattatggaa tatataaacc tgttgctgag aatttactac
     2041 aagataattg ggatgtatca tttgttgttt ctggaatgca tttatcccat aaatatggat
     2101 ttactaaaga attgatctta gctgacggat ttagaattag tggagaagtt caaagtttgt
     2161 ttttagagaa caataaggga aacatggctc ggtctgtggc attagagata aatggtttca
     2221 caaatatatt tgaacaaact agccctgatt ttgtattatt actaggggat agaggagaaa
     2281 tgttggcagc tgcgatagct tgtttgtatc ttggaatcaa aacagcacat attcacggag
     2341 gcgaagtgtc tggtactgta gatgaatcaa taagacatgc cataacgaaa atagcatctg
     2401 ttcattttgc tgctacggaa aaaagcaaag agcgtttaat aaaaatgggg gaagattctt
     2461 ggagagtttt tcccgtcgga gctcctagaa tagatactat tttaaataat agattaccta
     2521 gttttagaga tataaaagaa aaatatcatc taaaatttga aaagaaaaaa tacgttttgc
     2581 aagtatttca tccagttgta accgaactag aaaatataga aaaacaaatt ttagctttga
     2641 tcaattcaat ggaacaacaa tctcaaccaa ttatttgcat tttacctaat agtgatgctg
     2701 gtagcgaaat tatcagaaaa atgtaccaga aatatttaaa aacggatatt atttatatta
     2761 cgaatttaga gccggaggaa tacctgacta tattaaaaga gtgcaaattt atgatcggaa
     2821 attcttcttc tgggataatt gaa
//
LOCUS       DACDKM010000180         2815 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20834_2016
            SAMN12640370-rid9891663.denovo.183, whole genome shotgun sequence.
ACCESSION   DACDKM010000180 DACDKM010000000
VERSION     DACDKM010000180.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640370
            Sequence Read Archive: SRR10040891
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2815)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2815)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742835.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742835.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:04:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,012
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 29 of 75
            Pseudo Genes (incomplete)         :: 33 of 75
            Pseudo Genes (internal stop)      :: 19 of 75
            Pseudo Genes (multiple problems)  :: 5 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2815
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640370-rid9891663.denovo.183"
                     /isolate="E20834_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..1281
                     /locus_tag="IV334_002733"
     CDS             <1..1281
                     /locus_tag="IV334_002733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8944738.1"
                     /translation="VDIYYRSAIAEAIALTIIPAVLYYAYGLIFNREKKVIALSVSIS
                     LLVYTHVLTTLLCSALIACMIIVRLFLSKQIKNDIKFVVVNLVKAVFLTCALTSYFWY
                     PMLQQMNYQEIHSPFKTDLQLEALSISDSIIQALNNDITTYTMGLLGLVALIFPLFIL
                     KEMEKKEKIIYVGVVSTWVCVTNLFPWFLFQKTPISLLQFPWRILGIQIFFSSMIITL
                     VFMKKTMNKKYSWLIIGATVVFLLVTSIAAKENYAKVIQSKHGHIVINEETLPRYTTS
                     DMGGLYDYAPTEAIKERPHLEKHEVKVGESWEKGKYKAADNNITYTVASNKKQKVTVP
                     VYAYLGTQVKVNGKVVNDSYKKHGLVNVDVNAGENKIEITTKYTPTALMALWISILTY
                     CLVVYSSFKRFAPNKLKDSRYVTKKEINKKNENK"
     gene            1268..>2815
                     /locus_tag="IV334_002734"
     CDS             1268..>2815
                     /locus_tag="IV334_002734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8944739.1"
                     /translation="MKINKKVVILSGLIIVSSIYSFIFFKNTQSIYTTGDLSFHLSRI
                     KGLSTIFTSPINYETFNYTGYGVNYFYPFLTFFPAVMLYWMTKNLIVSYIIYVWLLNL
                     CTTLVAYHYGERFLKQKKAAFLFSCLYIFSAYRTVDIYYRSAIAEAIAITLVIPVLFY
                     AYQIISGKEEKYPSVKLALSMSLLVYSHVLSTLMSTALIIIFIFIRLVSKGFKNADFI
                     AIFKKLFSAAGMTLVLTSAFWYPMFEQMLYQKINKPSVTNLYAHASNVFDSLTEAMNN
                     DLTTYSMGLVGLLSLCIPLILFKKLTNIEKKIYYGTCLTWLATTSLVPWYLLQNTPAK
                     LLQFPWRILSLQIIFSSLILTMIFFKNRRYNKTRELFYLFMTVLLLITLTVSSKKNFN
                     QKIISQPGHIVVNKETVEAYTTKGMDYFLYDYSPIASLENKEHIIKHEAKIGSQWVNL
                     PYKTTGSSIIYRVTNEKSQVITLPVYAYLGTNVEINHQPVKEQVNANGLVQISGVKGV
                     ADIKVSTS"
BASE COUNT          963 a          425 c          487 g          940 t
ORIGIN      
        1 gttgatatct attatcgatc cgcaattgca gaagcaatcg ctcttacgat tatcccagct
       61 gttttatatt atgcttatgg attgatattc aatagagaaa aaaaagtcat cgctttatct
      121 gtcagtataa gtctacttgt gtatactcat gtcttaacta cactattgtg tagtgcattg
      181 attgcttgta tgattatagt tcgtttgttt ttaagcaagc aaataaaaaa cgatataaaa
      241 tttgtggtcg tgaatttagt aaaggctgtt tttttgactt gtgcattaac tagctatttt
      301 tggtatccta tgttgcagca gatgaattat caagaaatac acagtccctt caaaacagat
      361 ttgcagctag aagcactttc tatttcagat agtatcattc aagcgttaaa taacgatatt
      421 acaacataca ctatggggtt attaggattg gttgcgttga tttttccttt attcatactg
      481 aaagaaatgg agaaaaaaga aaaaatcatc tatgttggcg tagtttctac atgggtatgc
      541 gtaactaatt tatttccatg gtttttgttt caaaaaacac cgataagctt actccagttt
      601 ccttggcgta tcttaggtat ccaaatcttt ttttcttcaa tgatcataac gcttgttttt
      661 atgaaaaaga ctatgaataa aaaatattca tggttaatca ttggcgctac tgtggtgttt
      721 ctactcgtca cgtctatagc agcaaaagaa aattacgcaa aagttatcca atcaaaacac
      781 ggtcatattg ttatcaatga agaaacactc ccacgttata caacatcaga tatgggagga
      841 ttatacgatt acgccccaac agaagcgata aaagaaaggc ctcacttaga aaaacatgaa
      901 gtaaaagtag gagaatcgtg ggaaaaaggg aagtataaag ctgcagataa taacattact
      961 tacacagtgg caagcaacaa aaagcaaaaa gtgacagtac cagtatatgc ttatttaggt
     1021 acacaggtaa aagtaaacgg taaagtcgta aatgactctt ataaaaaaca tggcttagtg
     1081 aatgtggatg taaatgctgg agaaaataag atagaaatta caacaaaata tactccaact
     1141 gctctcatgg ctttatggat cagtattctg acttattgtt tagtggtgta ttcaagtttt
     1201 aaacgatttg cacctaataa gttgaaagat tctcgatatg tgactaaaaa ggagataaac
     1261 aagaagaatg aaaataaata aaaaagtagt aatattgagt ggattaatta tagttagtag
     1321 tatatattct ttcatctttt tcaaaaatac acagagtatt tataccactg gagatctttc
     1381 ttttcactta tctcgaatca aggggttatc gactattttt accagtccga ttaattatga
     1441 gacattcaat tatactggat atggtgtaaa ttatttttat ccctttctga ctttttttcc
     1501 agctgtaatg ttatattgga tgacaaaaaa tttgattgta agctatatta tttacgtttg
     1561 gttattgaac ttgtgtacaa cactcgtagc ttatcattat ggcgagcggt ttttaaaaca
     1621 aaaaaaagcg gcatttttat ttagttgtct ctatattttc tctgcttatc ggacagtaga
     1681 catctactat cgttccgcaa ttgccgaagc tatcgcaata acacttgtta tccctgtatt
     1741 attttatgct tatcaaatca tctctggaaa agaagaaaag tatccgtctg taaagctagc
     1801 attgagtatg agtttgctag tgtacagcca tgtactatca acattaatga gtacagcatt
     1861 aattatcatt tttattttta ttcgtttggt ttctaaaggt ttcaagaatg cagattttat
     1921 tgctatattt aaaaaattat ttagtgcggc aggaatgaca ttggttctga cgagtgcttt
     1981 ttggtatccg atgtttgaac aaatgctcta ccaaaaaatc aacaaaccta gcgtgacaaa
     2041 tttatatgct catgcatcta atgtgttcga tagtctgact gaagctatga acaacgactt
     2101 aactacttac tcaatgggac tagttggtct gttatcttta tgtattcccc tgattttatt
     2161 taaaaaatta acaaacatag aaaaaaagat ttattatggt acgtgtttga cttggctagc
     2221 tacaactagt ttggtgccat ggtatcttct gcaaaatacg ccagccaaat tgttacaatt
     2281 tccttggcgg atcttaagct tgcaaataat attctcttct ctgatattaa cgatgatatt
     2341 tttcaaaaat agaagataca ataagacaag agaactattt tatttattta tgactgtttt
     2401 gttattaatc actttaactg tttctagtaa aaaaaatttc aatcaaaaaa taatttctca
     2461 gccgggacat attgttgtaa acaaagaaac agtagaggcg tataccacta aaggaatgga
     2521 ttatttttta tatgattatt ctcctatcgc ttcattggag aataaggagc atataataaa
     2581 acacgaagca aagattggca gtcaatgggt aaaccttcct tataaaacaa ctggtagttc
     2641 tatcatatat agagtaacta atgagaagag ccaagtaatc acacttcctg tatatgctta
     2701 tttaggaacg aatgtagaaa taaatcatca acctgtaaag gagcaggtga atgctaatgg
     2761 attggtacaa ataagcggag tcaaaggagt tgctgatatt aaagtaagca caagt
//
LOCUS       DACDKM010000181         2768 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20834_2016
            SAMN12640370-rid9891663.denovo.184, whole genome shotgun sequence.
ACCESSION   DACDKM010000181 DACDKM010000000
VERSION     DACDKM010000181.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640370
            Sequence Read Archive: SRR10040891
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2768)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2768)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742835.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742835.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:04:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,012
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 29 of 75
            Pseudo Genes (incomplete)         :: 33 of 75
            Pseudo Genes (internal stop)      :: 19 of 75
            Pseudo Genes (multiple problems)  :: 5 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2768
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640370-rid9891663.denovo.184"
                     /isolate="E20834_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(172..1224)
                     /locus_tag="IV334_002735"
     CDS             complement(172..1224)
                     /locus_tag="IV334_002735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAP8944740.1"
                     /translation="METSQKISPKIFLNKVLAGTATGIIIGLIPNAVLGAILKYFSAY
                     PFAVTIAQLAVIFQLATPLIIGGLIALQFGFDPMRMMVVAGASFVGSGVVKFTPDLGE
                     TGVYVGAGTGDIINTMLTASIAVGLILLIGNSFGSVSIVLTPIVVGVGAGLLGFYLFP
                     FVTAITSAIGSLINNFTTLQPLLMSILIGCSFAFLIISPISTVAIGMAIQLDGVSAGA
                     AAMGVAATTFVLVVNSWKVNKSGVTIAIALGAMKMMMPNLFRKPVILIPCLVSAVITA
                     IPVALFSISGTPASAGFGVVGLVGPLASLDAGLNGFLVVLCWLVIPVFAALITQFLCE
                     KVFKLYDREEVFKFLG"
     gene            complement(1237..2175)
                     /locus_tag="IV334_002736"
     CDS             complement(1237..2175)
                     /locus_tag="IV334_002736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015081805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HAP8944741.1"
                     /translation="MKIAIAGAGAMGSRFGLMLHQSGNEVLLIDGWAEHVQQIKEHGL
                     QANFNGKEVEAKLPIVLQSEVEKEDQVDLIILFTKAMQLEKMLQDIQSLIKKDTEVLC
                     LLNGIGHEDIIEKFVPMENIYIGNTMWTAGLEGPGQVKLFGSGSVELQNLGDGKEAAA
                     KKLADKLSESGLNAHFSDNIHYSIYRKACVNGTMNGLCTILDVNMAELGKTSTAHKMV
                     ATIVNEFAKVAAVEKIELDVPEVIAHCESCFDPETIGLHYPSMYQDLIKNHRLTEIDY
                     INGAISRKGKKYGVATPYCDFLTELVHAKEDSLNVK"
BASE COUNT          906 a          589 c          415 g          858 t
ORIGIN      
        1 caatcaataa cataaaggtg agtttcttct acattttaaa aaatcgaatg gcactgaaga
       61 gttcagttgt cagcatcaat ctcctactca aaaaggcaaa aaaacgtaag tcagatgttg
      121 ttgacatctc acttacgcat ccattgattg aaaatttctt agtaaaatct actagcctaa
      181 aaatttgaag acttcttctc gatcatacag tttaaatact ttctcgcata aaaattgagt
      241 aattagtgcc gcaaaaacag ggatgactaa ccaacaaaga acaacaagaa agccatttag
      301 ccctgcatct aaagaagcca acggaccaac aagtccgacc acgccgaaac cagcagaagc
      361 aggcgtacct gaaatagaaa atagagcgac aggaatagct gtaatcactg cactaactaa
      421 acaaggaata agaatcacag gttttctaaa tagattaggc atcatcatct tcatagcgcc
      481 taaagcaatc gcaattgtca cacctgactt attaaccttc caagagttca ctactaaaac
      541 aaaagtggtt gcagctacgc ccatcgcagc cgcaccagca gaaacaccat ctagttggat
      601 tgccattccg attgctactg ttgaaatagg agagataatc aaaaaggcaa aggaacaacc
      661 aatcaagata gacattaaca gcggttgtaa ggtagtgaag ttattaatca atgatccaat
      721 tgcagaagta attgctgtta caaaagggaa taaataaaaa ccaagtaaac ctgcacctac
      781 acctacaaca atcggtgtta acacaatcga cactgaccca aaactatttc caatcagtaa
      841 aatcagcccg acagcaatcg atgctgtcag catcgtatta ataatatcgc ctgtaccagc
      901 accaacgtaa acacccgttt cacccaaatc aggtgtgaat ttgactacac cagaaccaac
      961 aaaagaagct ccggctacaa ccatcattct catcggatca aatccaaatt gtaaggcaat
     1021 caaaccacca atgatcaaag gtgttgctag ttgaaaaata actgctaact gtgcgatcgt
     1081 taccgcaaag ggatacgcac taaaatattt taaaatggca cctaatactg cattaggaat
     1141 caatccaata atgattcctg ttgctgtacc agcgaggact ttattcagaa aaattttcgg
     1201 tgatattttt tgtgaagttt ccattcaatc tctcctttat tttacgttca aactatcttc
     1261 ttttgcatgg acaagctcag tcaagaaatc acaataaggc gtcgcaacac catatttctt
     1321 cccttttcta gaaattgcgc cattgatata atcgatttct gttaatcgat ggttcttaat
     1381 caagtcttga tacattgaag gataatgtaa cccaattgtt tccgggtcaa aacaagattc
     1441 acaatgtgca atgacttctg ggacatctag ttcaatcttc tctactgctg ctactttggc
     1501 aaactcattg acaatcgtcg ccaccatttt atgagcagtc gatgtttttc ctagctcagc
     1561 catattaacg tctaagattg tgcatagtcc gttcattgtt ccgttcacac atgctttgcg
     1621 ataaatagaa tagtgaatat tgtcagaaaa atgagcattt aatcctgatt cagacaattt
     1681 gtctgctaat ttttttgcag ctgcttcttt tccatcacct aaattttgta attctactga
     1741 accacttcca aataatttga cctgaccagg accttcaaga cctgccgtcc acatggtatt
     1801 tccaatatag atattttcca ttggtacaaa tttctcaata atatcttcat gcccgatacc
     1861 atttaataga cataagacct ctgtatcttt tttgattaat gattggatat cctgcagcat
     1921 tttttccagc tgcatcgctt tggtaaatag aataatcaga tcaacttgat cttctttttc
     1981 tacttcggat tgaagaacga ttggtagttt tgcttctacc tctttaccat taaaatttgc
     2041 ttgtaatcca tgttctttga tttgttggac atgttctgcc cagccatcga ttaataatac
     2101 ttcgttccca ctttggtgaa gcatcaaccc aaagcgactt cccattgcgc cagcgcctgc
     2161 aattgctatt ttcattgatt cttcctcctt tttctttcta ttatactcat ttgagaatct
     2221 tttcacaacc tcgttttgtt attcttctct tatttatcat gtttatatcc accattcaag
     2281 ttattttatc agcaaatcaa taaaaactgg aatagaatcc caatttcatc gtaagagaaa
     2341 ctagactagc aataagaaaa tagaaacatt catagaaaaa atctcactcc tctgattata
     2401 aaagaggggg cattaacctc gtgtatacta aaaccttcca ttcattcgat ggatgatttt
     2461 ctttttgtga aaaaatattc attgtagtac gatgttgcgc aacacagacg gatggtattg
     2521 cttattattt attaaaatcg aacaatacac ttatttaaaa acttctttcc cagattttct
     2581 tccattttta cagttataac tagtattatc atgaaaaata gccttttatt gtctatggga
     2641 ataataattt aaaattggta cttttttatt aattagttca tcgcccaata atctctccag
     2701 tttcttagca tctcctgact caaccggaat tatccaagcc cagtctaaat aattttttaa
     2761 aatctcat
//
LOCUS       DACDKM010000182         2734 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20834_2016
            SAMN12640370-rid9891663.denovo.185, whole genome shotgun sequence.
ACCESSION   DACDKM010000182 DACDKM010000000
VERSION     DACDKM010000182.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640370
            Sequence Read Archive: SRR10040891
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2734)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2734)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742835.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742835.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:04:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,012
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 29 of 75
            Pseudo Genes (incomplete)         :: 33 of 75
            Pseudo Genes (internal stop)      :: 19 of 75
            Pseudo Genes (multiple problems)  :: 5 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2734
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640370-rid9891663.denovo.185"
                     /isolate="E20834_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(41..1840)
                     /locus_tag="IV334_002737"
     CDS             complement(41..1840)
                     /locus_tag="IV334_002737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HAP8944742.1"
                     /translation="MNYLKRSLGNAWDFLKGTQAYFRDVLLMHGFILFICLPLLSSTT
                     RFILHRGAIDYLSYDNIPSIFSQHPGVLLSLIVVLMTILLLVYFEFTFLLMSVFFIKK
                     KEPISLKQLLHLTILQLKKVRPITFLFFLAYFLLILPISGLSFNSDLLSKIKIPAFIM
                     DFIFANRWIIVSSFLLVYIFLGYIGIRLIFALPEMILRDRPFRAAIRESWSLTKSRLL
                     AITGQFIVIGGTILLLSSLGYIVVILAQSMVEQFFPDYALISAVFAMTLLQGILLFNI
                     VMSTVGIFYIIVDFMDDEGFLPEIPKWFIPQAPNLRFSALKNTGLTLFAVFFGIGVCL
                     YNMDYLTSAVQTKPVTVSHRGVSTQNGAQNTLEALEKTSRDYHPDYVEMDVQETKDGH
                     FVVMHDANLRHLTGVNGTPQDLTLKELTNLTVTENGVQAPVCSFDSYLEKAHKLNQKL
                     LIEIKTSKKDSDDLVERFVHKYRKVILENHHILQSLTYQTVSDLKEAEPNFYVGYILP
                     FNIVGPPVTPADFLTMEYSTINRNFINSAHQDGKEVYVWTINDSDAMSRMMFYGVDGI
                     ITDEMGLLNESVKQTDEEITYSDKLLNFVLGVG"
     gene            complement(1964..2560)
                     /locus_tag="IV334_002738"
     CDS             complement(1964..2560)
                     /locus_tag="IV334_002738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HAP8944743.1"
                     /translation="MNIAASRKLINFISIIGLGLSIALTIYFINLGVFKDLNALRGLV
                     GDSIILGPIIFVFIQILQVVIPIIPGGISTAAGVLIFGPYAGFIYNYIGICIGSIIIF
                     LLGRRYGKPFILSMISDKTYNKYIGWLDNQNRFEKLFALAIFLPVAPDDALCLMAGLT
                     NMSVKRYTLIILIAKPLSIFLYSMALIYGGQYLSGLLG"
BASE COUNT          967 a          515 c          470 g          782 t
ORIGIN      
        1 tttttgttgt ataaagactt ttttcattaa cgatttttga ttagccgact cccaaaacaa
       61 aatttagcag tttatctgaa taagtgattt cttcatctgt ttgtttcaca ctttcattta
      121 gtagtcccat ctcatcggtt atgataccat ccacaccata aaacatcatg cgactcatag
      181 catctgaatc gttgattgtc catacatata cttcttttcc gtcttggtgg gcagaattaa
      241 tgaagttacg gttgatcgta ctatattcca ttgtcagaaa atcagcaggc gtcacagggg
      301 gcccaacaat attgaaaggt aagatgtagc ccacataaaa gtttggttct gcttctttta
      361 aatctgaaac ggtttggtac gtcaaacttt gcaaaatatg atggttctct aagataactt
      421 tgcggtattt atggacaaat cgttcgacaa ggtcatccga atctttttta gaggtcttga
      481 tctcaataag taatttttgg tttagcttat gtgctttttc caaataagaa tcaaaagaac
      541 aaacaggggc ttgcactccg ttttcagtaa ctgtaagatt tgtcaattct ttcaatgtaa
      601 gatcctgtgg tgttccattt acccctgtta agtgacgaag atttgcatca tgcatcacta
      661 cgaaatgacc atcttttgtt tcctgaacat ccatctctac ataatcagga tgatagtctc
      721 tgcttgtttt ttcaagtgct tctaaagtat tttgcgcccc attttgggta ctcacacctc
      781 tgtgagagac agtcactggt tttgtctgaa cagctgaagt cagataatcc atattgtaaa
      841 gacagacgcc aataccgaaa aaaactgcaa aaagagtcaa accagtgttt ttcagcgcag
      901 agaaacgcaa gttgggggct tgtgggataa accacttagg gatctcaggc aagaagcctt
      961 cgtcatccat gaaatcaaca atgatataaa aaatccctac ggtagacatc acgatattga
     1021 ataataaaat cccttgtaaa agcgtcatag caaacacagc gctgatcaat gcatagtctg
     1081 ggaaaaattg ttcaaccata ctttgtgcca aaataacaac gatatatcct aaagaagaaa
     1141 ggagtaaaat cgtaccgcca atcacaataa attggcccgt gatggccaaa agacgcgatt
     1201 tggtcaatga ccagctttcc cgaatggctg ctcgaaaagg acgatctcgt agaatcattt
     1261 ccggtaaagc aaaaatcagt cgaatcccaa tatagccaag gaaaatataa acgagtaaaa
     1321 aggaactgac gatgatccaa cgattagcaa aaataaaatc cataatgaaa gcggggattt
     1381 ttatttttga cagtagatca gaattaaaac ttaatccgct gatcggtaga atcaataaaa
     1441 aataagcaag gaaaaacaaa aaagtaatgg gtctcacttt cttcagctga agtatcgtca
     1501 agtgtaagag ttgcttcaaa gagattggtt ctttcttttt aataaaaaaa acactcatca
     1561 aaagaaaagt aaattcgaaa taaactaaca gtaaaatcgt catcaaaact acgattaacg
     1621 agagtagaac cccaggatgt tgagaaaaaa ttgaaggaat attgtcataa gacaaatagt
     1681 caattgcccc tcggtgtaaa ataaaacggg tcgtactgct caataagggt aaacaaataa
     1741 ataaaataaa gccatgcatc agcaagacat cacggaaata agcttgtgtt ccttttaaaa
     1801 agtcccaagc attacctaag ctacgtttca aataattcat cctatcacct gctcttcaca
     1861 ttgtagcgca aatctaatga aaaaggaaag ggtgcagtgt gttttgcaga ccctttccca
     1921 gatttaacat tgtcaatcaa tgactaattt tacaaaataa atattatcca agaagaccac
     1981 ttagatattg acctccataa atcaaggcca tactatataa gaaaatcgac agtggtttag
     2041 caatcaaaat gattagtgta tatcgtttca ctgacatgtt tgtcaatcca gccatcaaac
     2101 ataacgcatc gtcaggagca actggaagaa aaatagctag agcaaacagc ttctcaaaac
     2161 ggttttgatt atccagccag ccaatatatt tgttatatgt cttatcgcta atcatggaaa
     2221 gtatgaatgg ctttccataa cgacgcccta gcagaaaaat gataatcgaa ccaatgcaga
     2281 ttccaatata gttatagata aatccagcat aagggccaaa tatcaggaca ccagctgcag
     2341 tactgatccc tccaggaatg attggaataa ctacttgcag gatttggatg aagacaaaaa
     2401 taatcggtcc taaaatgatg gaatcaccta ctaatcctct taacgcattc aaatctttga
     2461 aaacacctaa attaataaaa taaatcgtca gcgcgattga taggcctaat ccgataatgg
     2521 atatgaaatt tatcagtttt cgagaggcag caatattcat tattcatcaa ctccttagag
     2581 gttctttgtt aactatactt tactagaaaa gcaactaact gcgccatttt ttgatatgac
     2641 agaaaacaaa caattttgta agttcatcat aagtataaaa gattgatctc aaaaaaacat
     2701 ccaactaaag acttatttaa taacttgttt tttt
//
LOCUS       DACDKM010000183         2725 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20834_2016
            SAMN12640370-rid9891663.denovo.186, whole genome shotgun sequence.
ACCESSION   DACDKM010000183 DACDKM010000000
VERSION     DACDKM010000183.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640370
            Sequence Read Archive: SRR10040891
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2725)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2725)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742835.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742835.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:04:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,012
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 29 of 75
            Pseudo Genes (incomplete)         :: 33 of 75
            Pseudo Genes (internal stop)      :: 19 of 75
            Pseudo Genes (multiple problems)  :: 5 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2725
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640370-rid9891663.denovo.186"
                     /isolate="E20834_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            700..2610
                     /gene="ltrA"
                     /locus_tag="IV334_002739"
     CDS             700..2610
                     /gene="ltrA"
                     /locus_tag="IV334_002739"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HAP8944744.1"
                     /translation="MPKNKNETLCVEDLRHAEYYEMQNTFDDLYAKSKNGDIFTHLMD
                     IILSRENILLAYRNIKANAGSKTAGTDGTIIKDIGKLPAETVVKKVRYIVAGSPHGYR
                     PKPVRRKEIPKPNGKTRPLGIPCMWDRLIQQCIKQVLEPICEAKFSENSYGFRPNRSV
                     ENAIKATYNRLQISQLHYVIEFDIKGFFDNVNHSKLIKQIWAMGIRDKHLIFILKRIL
                     KAPIKMTNGTITYPEKGTPQGGIISPLLANIVLNELDHWVESQWQENPVTKNYVVHIN
                     KSGSPCKSNAYKEMKKTKLKEMYMVRYADDFRVFCRYKESAEKAKIAITQWIEQRLKL
                     EVSQEKTRIVNVRKRYSDFLGFKIKMIPRRKKLVVKSHISDKQFKNQKDKLVSQAKKI
                     ASPSKGKTELEETRLYNSMVLGMQNYYQIATNVNLDCMKLNRAVMAIFTNRLGTNKRG
                     RLRKTGRQLTTKEATRYGKSKMLRYVAGEGEPIFPVGYIQHRKPMAKVYLANCYTLEG
                     RKFIHTNLSVDKLLMYQLMKLSLENRTIEYADNRISLFSAQHGKCAITLEEFQQANEI
                     HCHHIVPTGAGGNDDYKNLILVKEAVHRLIHAKTEETIQKYIALLKLNKVQLIRLNKY
                     RVLAGNQELNLI"
BASE COUNT         1067 a          421 c          558 g          679 t
ORIGIN      
        1 aaaagctaag gcactaataa aagaacgaaa aagtttggtt attaggagaa ctgataatct
       61 gacaggtgga atgatgaagt aacgctctga aacgcctgcc ctgagcctcc gacaagcatt
      121 gtatgaggaa actcagaatg tttgaagctc ggtaaagacg gctgaaagat accgtaatag
      181 ttgatgaaca tatatcttct atcgaaaact atccagaggt gggtagcgta tccaacaggt
      241 cgggggtcta taaaatatct atggttagaa tgttctataa ggaactgacg aaacagcgaa
      301 tgtacgggtc taaatttgaa ctttgtagaa atgcaaaggt acgagaaatc gtagctggtg
      361 tggcaaagta agaatatagg ttatgaaatg ccatataacg ttacaggcgg tatcaagcca
      421 gcaggcttat agctagtgac ctaaggatat atatgtatag ataagattat cggaacgagg
      481 aaaggtattg ggttctcatc aagagaataa actgacgaag aacaataagc agttgaacta
      541 atgctgaaaa gcagagggcg aacctaagaa attcttgtaa tgagaatgga ggaatgccct
      601 caagtcgtgt atatattaga aaagtattat gttgtttcaa taaggattac gagtaagata
      661 aaaaagctca ctcaatttca agtaaaggag tgaagcctta tgcctaaaaa taaaaatgaa
      721 acattgtgtg tagaagattt aagacacgct gaatattacg aaatgcaaaa cacctttgat
      781 gatttatatg ccaaaagtaa gaatggagat attttcactc atcttatgga tattatccta
      841 tcgagagaaa atatattact ggcatatcga aatatcaaag caaatgcagg cagtaaaaca
      901 gcaggaacag acggtacaat aattaaagat attggcaaat tgccagcaga aacagttgtc
      961 aaaaaagtaa gatacattgt tgcaggcagt ccacatgggt atcgacctaa gccagtgagg
     1021 cgaaaagaaa taccaaaacc aaatggtaaa accagacctc taggtattcc ttgtatgtgg
     1081 gacaggctta tacaacaatg tataaaacaa gtattagaac caatctgcga agctaaattt
     1141 agtgaaaaca gctatggttt tagacctaat aggtcagttg aaaatgcaat aaaagcaacc
     1201 tataaccgcc tacaaatcag ccagctacat tatgtgattg aatttgatat taagggtttc
     1261 tttgacaacg tgaaccattc caagctgata aaacagatat gggctatggg aatacgggac
     1321 aagcacttga tattcatttt aaaaaggata ttaaaagctc caatcaaaat gactaacggt
     1381 acaataacat atccagagaa aggaactccg caagggggaa taatatcccc actactagca
     1441 aacattgttc ttaatgagtt agaccattgg gtagaaagcc aatggcagga aaatcctgtt
     1501 acaaaaaact atgtggttca tatcaataaa agcggtagcc catgtaaaag caacgcttac
     1561 aaagaaatga agaaaacaaa gttaaaggaa atgtatatgg ttcgttatgc agatgatttt
     1621 agagttttct gccgctacaa ggaaagtgct gaaaaagcca aaatagcaat cactcaatgg
     1681 attgaacaac gattaaaatt ggaagtatca caagaaaaaa caagaattgt aaatgttaga
     1741 aaaagatact ccgactttct tggtttcaag attaaaatga taccgagaag aaagaagtta
     1801 gttgttaaat cacatataag tgataaacaa ttcaaaaacc aaaaagacaa gctggtatca
     1861 caggcaaaga aaattgcttc tccctcaaaa gggaaaacgg aattagaaga aacaagactt
     1921 tataattcaa tggtattagg tatgcaaaat tattatcaga ttgcaacgaa tgtaaatctg
     1981 gattgtatga aactgaatag agctgtaatg gctatcttca ctaacagatt aggtacaaac
     2041 aaaaggggca gattgagaaa aacaggcaga caacttacga caaaggaggc aacaagatat
     2101 ggtaaatcta aaatgcttcg gtatgttgct ggcgagggcg aacctatttt ccctgttgga
     2161 tatatccaac acagaaaacc aatggcaaag gtgtatttag cgaattgcta cactctagag
     2221 ggaagaaaat ttatccacac caatctaagt gttgataaat tactgatgta ccagttaatg
     2281 aaattatctt tggaaaatcg cacaatagaa tacgcagaca atcgaatttc gctattttct
     2341 gctcaacatg gaaaatgtgc tataactctg gaagaatttc aacaagctaa tgaaatacat
     2401 tgtcatcata ttgtaccaac tggagctggt ggaaatgacg attataaaaa tcttattttg
     2461 gtaaaagagg cggtacaccg tctaatacac gctaaaacag aagaaacaat acaaaaatat
     2521 atagctctat tgaagctaaa taaagtccag ctcataagac taaataaata tagagtatta
     2581 gctggcaatc aagaattaaa cttgatatag gtctactaca taaatacttt tcaatataaa
     2641 cacgatggaa cgcggagtgc ggtgaaagtc gcacgctccg tgtgaatggg gggaaaagtt
     2701 tgagatattt ataatatcag gaact
//
LOCUS       DACDKM010000184         2723 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20834_2016
            SAMN12640370-rid9891663.denovo.187, whole genome shotgun sequence.
ACCESSION   DACDKM010000184 DACDKM010000000
VERSION     DACDKM010000184.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640370
            Sequence Read Archive: SRR10040891
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2723)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2723)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742835.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742835.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:04:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,012
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 29 of 75
            Pseudo Genes (incomplete)         :: 33 of 75
            Pseudo Genes (internal stop)      :: 19 of 75
            Pseudo Genes (multiple problems)  :: 5 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2723
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640370-rid9891663.denovo.187"
                     /isolate="E20834_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..386
                     /locus_tag="IV334_002740"
     CDS             <1..386
                     /locus_tag="IV334_002740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8944745.1"
                     /translation="EEIEWNNLTQNNKNNSGCGIIFMFVIVLGIVVLLITVKIVDYFA
                     NTPEEQIQRKLPEFERVPPYDPRDNSFQVKKLKESPNEMWFEVTVESSDFKEKKKEYN
                     YIVKAKVSKRFIFNPKLEIYSVVIE"
     gene            711..1328
                     /locus_tag="IV334_002741"
     CDS             711..1328
                     /locus_tag="IV334_002741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001224538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAP8944746.1"
                     /translation="MRKFFYIRVSSYDQSIERQLISAKELGIPEEYIFVEKASGKDFK
                     RPEYQLMKRMFRKGDLLYLQSLDRLGRNKQMILEEWRELTKEKQIDISVLDMPLLDTA
                     SYKQLDGLETLISDLILQLLSYMAEDERKRINSRQREGVEAAKQKGVKFGRKKIALPP
                     EFPAIYSDWKAGKITAVYAMDQLGMKRNTFYRRVKEYEHTLHHFS"
     gene            1369..1668
                     /locus_tag="IV334_002742"
     CDS             1369..1668
                     /locus_tag="IV334_002742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000366408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8944747.1"
                     /translation="MDLTNQRVVFTGTLQQMTRTQAIGLVHSLNGHCQSSVTSKTNFL
                     VCGQYSKSLFDDSLYTKKEQTARDLIALGHEITILSEVEFYALISQQLKEWQRYL"
     gene            1702..1869
                     /locus_tag="IV334_002743"
     CDS             1702..1869
                     /locus_tag="IV334_002743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000824191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAP8944748.1"
                     /translation="MKRKSYDRWGIEITSFNRNIAFHVECDCGELAKSVYQKNYFYCP
                     KCHRRYGVHGT"
     gene            complement(1914..2519)
                     /locus_tag="IV334_002744"
     CDS             complement(1914..2519)
                     /locus_tag="IV334_002744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAP8944749.1"
                     /translation="MGKIGYIRVSSQDQNLDRQLAMMEGLNIDKLFQEKASGKDTERP
                     EFQKLLNYIREGDCVIVTSLDRLGRDYEDIKNTVAFMKQKKVALKILDAQFLDFNTGN
                     ELLDTAMFDMFLSLLSYIAQNEREKIRERQRQGVLLAQKAGRYKGRPIEYSINSTDPQ
                     KRLVFKTVVDMLKQGIPVAEIAKENGISRPTIYKIKKANDL"
BASE COUNT          955 a          409 c          498 g          861 t
ORIGIN      
        1 tggaagaaat agaatggaat aatctgactc aaaataataa aaataattct ggatgtggaa
       61 ttatctttat gtttgttata gttttgggaa tagtagtact tttaataact gtcaagatag
      121 tagattactt tgcgaatact cctgaagaac aaatacagag aaaacttcct gagtttgaaa
      181 gagtaccacc ttatgatcca agagataatt catttcaagt aaaaaaatta aaagagtctc
      241 caaatgaaat gtggtttgaa gttacagttg aatcaagcga cttcaaagaa aaaaagaaag
      301 aatacaatta tattgtaaag gcaaaagtat caaaaagatt tatttttaac cctaaattag
      361 aaatatatag tgttgtaata gaataatagt gttaaaaaaa ttgtaaaagt taaagaagtc
      421 aaattctgat gtgaaaaata aaatatacca tggaagacat aaaagaaaaa ctggataact
      481 agtttattta atggcagacc gttaaggcct gccattttta tgtattttag cggaataaat
      541 ataatcatgc gggagtgtaa acttactgtt tcaaaaagta tactttttga catgccttga
      601 ttttctttgt ttatgtatgg cataataacc acataataca atttgaaacg tttctgattt
      661 aatctgattg ttttgaaaca attttagtga aaaaagggta gataaaaacg atgagaaaat
      721 ttttttatat ccgggtcagt agttatgatc aatcgatcga acgacaactg atttccgcga
      781 aggaattagg aatccctgaa gaatatatat ttgtagaaaa agccagcggc aaagatttta
      841 aacgccctga atatcaatta atgaaacgga tgtttcgaaa aggagattta ctctatctac
      901 aatcattaga tagactaggc agaaataaac aaatgatttt agaagaatgg cgagaattaa
      961 ctaaggaaaa gcagattgat atttctgtgt tagacatgcc tctgttagat actgctagtt
     1021 acaagcaact agatgggtta gagacgctta tctctgactt aattctgcaa ttactcagct
     1081 atatggccga agacgagcgg aaacgaatta atagccggca aagagaaggc gtagaagctg
     1141 cgaaacaaaa gggtgtaaaa ttcggcagaa agaaaattgc tttacctcct gaatttcctg
     1201 cgatctattc tgactggaaa gcaggaaaga taactgcagt ctatgctatg gatcaattgg
     1261 gcatgaaacg aaataccttt tatcgacggg tgaaagaata tgaacatacc ttgcaccact
     1321 tttcttgata gatataacag ctattagacg ggaagggaga gtttgaaaat ggacttaacg
     1381 aatcaaaggg tcgtttttac tggaacgctg cagcaaatga cacgaaccca agccatcggt
     1441 ttggttcact ctttaaacgg ccattgtcaa tcaagtgtta cttcaaaaac aaatttcctg
     1501 gtttgtggtc agtattctaa gtcgttgttt gatgattctc tctatacaaa aaaggaacaa
     1561 accgcgcggg acttgattgc gttaggtcat gagattacca ttttatcaga agtcgaattt
     1621 tatgcgttaa ttagtcaaca gttgaaagaa tggcaacggt atttataatc gttagtaaaa
     1681 tttacaagga ggaaaatttt tatgaagcga aaatcttatg atcgttgggg cattgaaata
     1741 actagtttta atcgaaatat tgcttttcac gtggagtgtg actgtgggga attagcaaaa
     1801 agtgtttatc aaaaaaatta cttttactgt cctaaatgcc atcgccgata cggggtacat
     1861 ggaacataga aacgaaatag tgcggtagaa aagcgaatga atgtagtata tttctacaaa
     1921 tcattcgctt tctttatttt ataaatagtt ggtcgagaga tcccgttttc ttttgcaatc
     1981 tctgcaactg gaatgccttg ctttaacatg tccaccacgg ttttaaaaac taagcgtttt
     2041 tgcggatctg ttgagttaat agaatactcg attggtcgtc ccttatatcg gccggctttt
     2101 tgagccaata atactccttg ccgctgacgt tcacgaattt tctcacgctc gttttgtgca
     2161 atataactta gcagtgataa aaacatatcg aacatagcgg tatccaggag ttcatttcca
     2221 gtattaaaat ctaaaaactg tgcatctaat atttttaacg ctactttttt ctgtttcata
     2281 aaagcaactg tattttttat atcttcataa tctcttccta aacgatctag tgatgtaacg
     2341 atgacgcaat caccttcacg gatataattt agtaattttt gaaactcagg acgctcagta
     2401 tctttaccac tggctttttc ttggaataat ttatcaatgt taagtccttc catcattgcc
     2461 aattgtcgat ccaaattttg atcttgtgaa gatactcgaa tatagccaat ttttcccata
     2521 aaaaactcct ttttaaaagt gatataattt acatgtaaaa tttacttgta gttttactgc
     2581 tatcatagca tttttaaaaa taaattttat gtagacttaa aatttacttc atggagggtt
     2641 taaatggatg aaatcaaaag taacaaaaaa gcatttggca tttatttagg agaacctagt
     2701 acagaacaat taaaccaatt ttt
//
LOCUS       DACDKM010000185         2689 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20834_2016
            SAMN12640370-rid9891663.denovo.188, whole genome shotgun sequence.
ACCESSION   DACDKM010000185 DACDKM010000000
VERSION     DACDKM010000185.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640370
            Sequence Read Archive: SRR10040891
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2689)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2689)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742835.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742835.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:04:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,012
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 29 of 75
            Pseudo Genes (incomplete)         :: 33 of 75
            Pseudo Genes (internal stop)      :: 19 of 75
            Pseudo Genes (multiple problems)  :: 5 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2689
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640370-rid9891663.denovo.188"
                     /isolate="E20834_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            174..518
                     /locus_tag="IV334_002745"
     CDS             174..518
                     /locus_tag="IV334_002745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HAP8944750.1"
                     /translation="MAEKQTAGRDQLGDFAPKFAELNDDVLFGQVWSREELSAHQRSI
                     ITISALISGGNFEQLPAHLKIGKQNGITSEEIAEIITHLAFYVGWPKAWSAFSLAKEI
                     YLEENSEDKKEA"
     gene            519..938
                     /locus_tag="IV334_002746"
     CDS             519..938
                     /locus_tag="IV334_002746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HAP8944751.1"
                     /translation="MTTKHEEIHNGVIFPAGEKNEAYAQVFTGQSYLNMLVNDPEVSV
                     GVGNVIFEPGCRNHWHVHKDGYQILLVTGGEGWYQEEGKPARRLMAGDVVVTHKGIKH
                     WHGATKDSWFEHLAITAGNSEFLEPVTDEVYEGLENQ"
     gene            complement(1030..1380)
                     /locus_tag="IV334_002747"
     CDS             complement(1030..1380)
                     /locus_tag="IV334_002747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8944752.1"
                     /translation="MEKELSKRIIDELLTYLYSHEITEIRIGVEFAEEGLFIEIQGTA
                     ETQPEDLDVLADLLNNPRDPSMEIYYQELLGNPRHDREDYHLLGSLIDDVEIMYAQPI
                     INIKIFRRASHSYY"
     gene            complement(1346..2566)
                     /locus_tag="IV334_002748"
     CDS             complement(1346..2566)
                     /locus_tag="IV334_002748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1576 domain-containing protein"
                     /protein_id="HAP8944753.1"
                     /translation="MLSNTPTDNWSGLLTILTSPSNLLTDYFALGGFGSAFINVGILT
                     LLSVLLAYRHKVILNGPLFASILTVTGFSFFGKNLYNSISIILGVYLYAVFVNKPFSQ
                     YIMIGLFGSALSPVVSYITFGMRFPLLIGILLGNLAGIAIGLLLPPLAAQTLVFHRGF
                     TLYNIGFTSGLIAMTFTAVLRLFSYSIVENTLVFNEYHFPLIWIIFGFFSLTVGIGFY
                     YNSFRLLGIREIFDSSGKLTTDFIANSGIGATLINIGLVGLMLSSYVLLVGGQLNGPV
                     IGAILSAVGFSAFGCHLKNSFPILVGIFIASLFGTFHEITSTGMLVAAVFGTGLAPIS
                     GFYGSFYGVIAGVLHIALVHNVSTLHGGLNLYNSGFSTGFVAGILVPILDNFTAVRKE
                     KKTLGKRIIKKNHR"
BASE COUNT          998 a          487 c          523 g          681 t
ORIGIN      
        1 aaataaaaaa agcgttagca actatgctta aaaagcatat cgactgaata aactaagcat
       61 tggacaggct gaaagactac atttaaaatg aaatgcgaag aaacaataga aaataaagtt
      121 gccttagaac catagttgaa tcacctaaca gtcgtgaagg gagaataaga gaaatggcag
      181 aaaaacaaac cgcaggacga gatcaattag gagattttgc accaaaattt gcagaattga
      241 atgatgatgt tttatttgga caagtatggt ccagagaaga attatctgct catcagcgta
      301 gtatcatcac catatctgca ttaatttcag gaggaaattt tgaacaattg ccagctcatc
      361 tgaagattgg caagcaaaat ggcattacta gtgaagaaat agcagaaatc attacccatt
      421 tagcttttta tgtaggatgg ccaaaagctt ggtcagcttt tagtttagca aaagaaatct
      481 atttagaaga aaatagcgaa gataagaaag aggcgtaaat gacgacaaaa cacgaagaaa
      541 tacacaatgg cgtgattttt ccagcaggtg aaaaaaacga agcttacgct caagtattta
      601 ccgggcaaag ctatctgaat atgttagtga atgatcccga agtttctgtt ggtgtaggta
      661 atgtaatttt tgaaccaggt tgtcgcaatc actggcatgt ccataaagac ggctatcaaa
      721 tcttacttgt gaccggagga gaaggctggt atcaggaaga aggaaagcca gcaagacgat
      781 taatggctgg agatgtcgtc gtaacgcaca aagggattaa acattggcat ggtgcgacaa
      841 aagacagttg gtttgaacat ttggcgatca ctgctggtaa ttcagagttt ttagaacctg
      901 taacagatga agtgtatgaa ggattggaaa atcaataatg tcaagcttga acagctcctg
      961 aaagtagatt ttaatcgaac tttcaggagc tgcttttttg attcagtctt ttataaatag
     1021 aaaatagggt taataatagg aatgagaagc acgtcgaaag atcttaatat tgataatcgg
     1081 ctgggcatac atgatttcca cgtcatcaat caaactacct aacagatgat aatcttcccg
     1141 atcatgtcgg ggattaccta atagttcttg atagtagatt tccatactag ggtcccgagg
     1201 attgttcagc aaatcagcaa gtacatctaa atcttctggc tgagtttctg ccgtaccctg
     1261 tatctctatg aaaagccctt cctcagcaaa ttctactcca atacgtattt cagttatttc
     1321 atgggaatat aaataagtaa gaagctcatc gatgattctt tttgataatt ctttttccaa
     1381 gtgtcttttt ttcctttctg acagcggtaa agttatctag tatcggtaca aggatacctg
     1441 caacgaaacc agttgaaaaa ccactgttat atagatttag tcctccgtgc aaagtgctga
     1501 cgttatggac aagagcaata tgtagtacac cagctattac accgtaaaaa ctgccgtaaa
     1561 aaccgcttat gggcgcgaga cctgtcccaa acacagctgc aacaagcatg ccagtactgg
     1621 tgatctcatg aaaagttcca aacaaagaag caataaatat tccgactagt atcggaaaac
     1681 tgttttttaa atgacaacca aaagcactga aacccaccgc agataaaata gcaccgatga
     1741 caggtccatt taactgaccg ccaaccagca aaacataaga agacagcatc agaccaacta
     1801 agcctatgtt gatcaaagta gcaccaatac cagaattagc aatgaaatca gttgtcaatt
     1861 ttccagatga atcgaaaatt tctcgaatcc ccaaaagccg aaaggaatta taatagaaac
     1921 caattccgac tgtcagcgaa aaaaagccaa aaataatcca aatgagagga aagtgatatt
     1981 cattgaaaac caatgtattt tcgacgattg aatagctgaa taacctcaaa acagcagtaa
     2041 aagtcatcgc aatcaaccca gaggtgaatc caatattata aagtgtgaat ccacgatgga
     2101 aaactaaagt ttgagcagct aagggcggaa gaagtagacc aattgctatg ccagctaaat
     2161 tccccaataa aatacctatc agtaaaggaa aacgcatacc aaaagtgata taactaacga
     2221 ccggtgataa tgcgctacca aacagtccga tcatgatgta ctgagaaaaa ggtttattga
     2281 caaatacagc gtatagatac acacctaaaa tgattgagat agaattataa aggtttttgc
     2341 caaaaaaaga gaaaccggta actgttagaa tcgaagcaaa taagggaccg tttaaaatca
     2401 ccttgtgccg ataagctaaa agcacagaaa gtaaggtcaa aatccctaca tttatgaatg
     2461 cgcttccgaa accgcctaaa gcaaaataat ctgtcaaaag attgcttggc gaggtaagaa
     2521 tagttagcaa tccactccaa ttatcagtag gagtattaga aagcaagcca ataaaaagaa
     2581 aaaaacacct gtaaaaccaa gataaaaata ctttcttttc tgtgaaacga ttcgtttttg
     2641 aggatgaata taaataacgt cagtttcgtt ttccattaca caccttctt
//
LOCUS       DACDKM010000186         2672 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20834_2016
            SAMN12640370-rid9891663.denovo.189, whole genome shotgun sequence.
ACCESSION   DACDKM010000186 DACDKM010000000
VERSION     DACDKM010000186.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640370
            Sequence Read Archive: SRR10040891
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2672)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2672)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742835.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742835.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:04:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,012
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 29 of 75
            Pseudo Genes (incomplete)         :: 33 of 75
            Pseudo Genes (internal stop)      :: 19 of 75
            Pseudo Genes (multiple problems)  :: 5 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2672
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640370-rid9891663.denovo.189"
                     /isolate="E20834_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..322
                     /locus_tag="IV334_002749"
     CDS             <1..322
                     /locus_tag="IV334_002749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP8944754.1"
                     /translation="YKAIIPENLDEVKLYLWEDNGEIVGFSGVSAEELVMLFLDPDFI
                     GKRYGSQILIELIETEDIKRIDVNSQNKYAKHFYLSHGFEIESEEDLDGFGKPYPITH
                     LVKK"
     gene            736..1554
                     /locus_tag="IV334_002750"
     CDS             736..1554
                     /locus_tag="IV334_002750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HAP8944755.1"
                     /translation="MNKNPHNLKLVAVDMDGTFARTDYTYDIPRFKAVLSRMKAVDCQ
                     FVVASGNQYYQLRSLFPNYYDELSFVAENGAFVKDKTEVLFSADMPRKAIEVVLDLCK
                     DYPEVKNVMCGLNSAYCERGKVSQAFFDLTNIYYHRLEWVADFAEVQDKILKFAPTMP
                     VEKTEFYVELFREKLAGLLVPTSSGHGSVDLILSECHKTFGLKMLVERLGINPDQCVA
                     FGDGGNDIEMLEYCGLSYEMDNATEAVKQVAKHQCPSNDEDGVLVTLDRLFPNT"
     gene            complement(1627..1986)
                     /locus_tag="IV334_002751"
     CDS             complement(1627..1986)
                     /locus_tag="IV334_002751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HAP8944756.1"
                     /translation="MSDNLFLEVQPEVFKQVPRLKDGFLLTLNDGSNQFSSAEGCCMI
                     GDHFLLVSTQHYIPAYDLTIPCEEATVKTSEYDTTFINGHLVLEVTPSNQTIRLKNES
                     GILDSNVQVYFDSSDAS"
BASE COUNT          925 a          372 c          471 g          904 t
ORIGIN      
        1 ttataaagca atcattcctg agaacttaga tgaggttaaa ctttatttat gggaagataa
       61 cggagaaatt gtcggtttta gtggtgtaag tgcagaagaa ttagttatgc tatttcttga
      121 tccagatttt attggtaagc gatatggtag tcaaatatta attgagctga ttgaaactga
      181 agatataaaa agaattgatg ttaatagtca aaataaatat gcaaaacatt tttatttaag
      241 ccatggattt gaaattgaat ctgaagaaga cttagatggt tttggtaaac cataccctat
      301 aacgcattta gtaaaaaaat aattcatgaa tggtttatta cctgattgaa catgaataaa
      361 agtaatattt ttattttgat ggcctatttc taggttatga attatccaga tcaaatttgt
      421 gtattacatt cagtaataag gtgcttatct taggatcaac ttatgatact agctgaccaa
      481 ggatgtacca gttatagaaa atcagttaaa gtaaaaatga taaaaatctt atgtcaacac
      541 tgatttaggc tgacctgtgg gttaggagaa agacaggtcg gtagctttta atacaaaaaa
      601 tggcttgtat gtatctagat agttaactga aattagaaat gttcttattt ttaacggagt
      661 aagaaaaata ggctgcttta ggtctgaata ctttttcaat ataatgaaga tatcaaaggc
      721 gacaggagtg tttttatgaa taagaatcct cataatctaa agttggtagc agtagatatg
      781 gatggaacat ttgctcgtac tgattacaca tacgatatcc caaggttcaa ggctgtttta
      841 tcgcgaatga aagctgttga ttgtcaattt gttgttgcaa gtggaaatca atactaccaa
      901 ttgagatcgt tatttccaaa ttattatgat gaattaagtt ttgttgctga aaatggtgct
      961 tttgttaaag ataaaacaga ggtactattt tcagccgata tgcctagaaa agcgatcgag
     1021 gtggtccttg atttatgtaa agactatcct gaagtaaaaa atgtgatgtg tggtctgaat
     1081 agtgcctact gtgaaagagg aaaggtgagt caagcatttt ttgatctaac caatatctat
     1141 taccatagat tagaatgggt agctgatttt gcagaagttc aagataaaat tttaaaattt
     1201 gctccgacta tgcctgttga aaaaaccgaa ttttacgttg aattgttccg tgaaaaatta
     1261 gccgggctat tagtacctac cagtagtggt catggttctg tagacttgat tctttctgag
     1321 tgccataaaa ctttcggctt gaaaatgtta gttgagcgct tggggattaa tcctgaccag
     1381 tgcgttgctt ttggagatgg gggcaatgac attgagatgt tggagtattg tggtctgagt
     1441 tatgaaatgg ataatgcaac ggaagctgtt aagcaagttg ctaagcatca atgtccttca
     1501 aatgacgaag acggtgtttt agtgacgctc gatcgattgt tccctaacac gtaaacacta
     1561 ggtaattcat caaaaaacct cgatataaga aatattcgag gttttaaaac tatatttaga
     1621 acagatttac gaagcgtctg atgagtcaaa atagacttgg acattgctat ctaaaatacc
     1681 actttcattc tttaaacgaa ttgtttgatt tgatggagtt acctctaata ctaaatgccc
     1741 attaataaag gtagtatcgt attcagatgt tttaactgtt gcttcttcgc aaggtattgt
     1801 taaatcatat gctggaatat aatgttgtgt ggaaactaat aaaaaatgat caccaatcat
     1861 acagcatccc tctgctgaag aaaattgatt agagccatca tttaaagtta acaagaatcc
     1921 atcctttaaa cgaggtactt gcttaaatac ttctggttgt acttctaaaa ataaattatc
     1981 gctcattttc acgaccacca tcgacttcca cagttgaaat tgacatttta tttaacacac
     2041 ctattccccc ttaactataa ctcaattcta tataaaaaat attctattct caaataattg
     2101 aacttttcaa atcgccaatt tacttcggct ttatcagagc tcattcatat caagagcgat
     2161 taacgctgta aaaattaaac cgaagatctg ataaattttc tctattttct acaatattta
     2221 aaacgcttcc ttagtctatt atatatctat ttctcttagg agtatgttta gatgtttgaa
     2281 taatagttgc ccaactttaa aggttattct tataatattt tacttcattt tccaatagaa
     2341 aattcaaagg attactggtt tttctaaaat agtttagttt aaatacataa gtatcctccg
     2401 acactgtatt agttcataat catctaaata caaaattttc aaataaaaat atttcaagct
     2461 ctatcgttga cgaatgtaaa cgatacttat aaagtaatag aaattgatta catttgtaaa
     2521 cttaaaatgg ttaatttatc tcacataaaa ctaagaataa aataatgatt aaaagtggtt
     2581 agtttttata cgatttagcc tatcggtaaa aatgaattag agagtgtaaa atattttgtg
     2641 taaatgaaaa aatccataca aaaaaggaag tc
//
LOCUS       DACDKM010000187         2587 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20834_2016
            SAMN12640370-rid9891663.denovo.190, whole genome shotgun sequence.
ACCESSION   DACDKM010000187 DACDKM010000000
VERSION     DACDKM010000187.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640370
            Sequence Read Archive: SRR10040891
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2587)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2587)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742835.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742835.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:04:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,012
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 29 of 75
            Pseudo Genes (incomplete)         :: 33 of 75
            Pseudo Genes (internal stop)      :: 19 of 75
            Pseudo Genes (multiple problems)  :: 5 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2587
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640370-rid9891663.denovo.190"
                     /isolate="E20834_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            75..470
                     /locus_tag="IV334_002752"
                     /pseudo
     CDS             75..470
                     /locus_tag="IV334_002752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317574.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
     gene            790..1104
                     /locus_tag="IV334_002753"
     CDS             790..1104
                     /locus_tag="IV334_002753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP8944757.1"
                     /translation="MELEVKTNVNSLEIEFYSILQKKLEDYTEKLLAVSHLPLVLTNT
                     DLKRLFQISDSTLNRLVKLGEFPPCWYGIRGHYFRDDVLEWMRKSDSNDYREKLRLLR
                     SL"
     gene            1208..2386
                     /locus_tag="IV334_002754"
     CDS             1208..2386
                     /locus_tag="IV334_002754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAP8944758.1"
                     /translation="MEKTDRFKKQISSTGRITWYFQAYRTTRRGFNSKREAQLAYLEM
                     EKQQRLKKKIVASNDKFSVVGEQWFQYYRSLNEQKEITYDKREEQLKIIVRWIGDKKL
                     SDLTPECLQELLFTLKECGVNGTNVGYAKNSLQSLIQVLNMIFKYCQRKNLISENPMK
                     SVRMPKYQLTVTDLKEAINNIEDKFLTVDELRTFLNYGIVHEELPMSVLFHVLFYTGC
                     RVSEALALQPGDIDFKRNEILFYKQTAVKGKSKDFRIETTKTVSSARRVLVTPLVMEK
                     LQQLIKALGEMRQHYRFIIEETYLFVYLDSSKRGVPYRREYVNDHVKRCVNRSGINKP
                     FHTHLARHTMASLVAEHCSWEVLKDRLGHTDQTTSKIYRHLTSNEKVKPLIAFSSLES
                     "
BASE COUNT          844 a          380 c          529 g          834 t
ORIGIN      
        1 tcctagattg tagtaataat ggaaagggat agaagtaaaa cgagttgaat gaattttttg
       61 agggggagaa tattatgggc gactcagagg aaacccacat tcaacatacg tttgacagtt
      121 tttgtaagaa agtagtctgc aatgaggcgt taaatattca aaagaagtac gctagatttc
      181 gccaacggca aatatcaatg gaaatattgg aacaaaaagg gttaggtaca gagttctatt
      241 atttggaacc aaatttgaat agtagtggta cttttattgt ccttggtatg aaaatcttta
      301 tttccaatga ggagttggca aatgcgattg cagcattacc tagtcaaaga caagaaatca
      361 tttttatgtc cttcttcatc ggtcttaatg atcgtgaaat tggcgatatt attgggaaaa
      421 gtctcggcag tgtttggtac caacgacaaa tggctttgga agagctagga tttccatatt
      481 tcgattttgg ttggcatatt tttcatgcca aattctgtga ttttaatagc atcggcaaga
      541 tttaattttt cttcagctct ctctccactt ttaagttttt ttactgtact caagttgatt
      601 cctgttccga atgctattgc attagccgta gatttttcaa gaatttcttt aattattttt
      661 atgtctgcaa ttccttgttt catattaaaa attcctcttt tctagtccgt tttgatggag
      721 tactggagtg tttttttaca attttaaggg gtaccgtggt ggtcgttttg attttgagag
      781 agaagggtga tggagttgga agttaaaaca aatgttaatt ctctggaaat agagttctat
      841 tcaattttac aaaaaaaact ggaagattac acggaaaaat tgcttgcagt cagtcattta
      901 cctctggtat taacaaatac tgatttaaag cgactatttc agataagtga tagtacactg
      961 aaccgtttag taaaacttgg tgagtttcct ccctgttggt acgggattcg aggacactat
     1021 tttcgtgatg atgttttaga atggatgaga aagagtgatt cgaatgacta tcgagaaaaa
     1081 ttgcgactac ttcgatcttt ataagttaca ttttctttct aagatgcggt acaatgatga
     1141 catagataga aagatttcca aagggtttct gcttgttggg ttccttcaaa ataggaggaa
     1201 tcaataaatg gaaaaaacag atcgattcaa gaagcaaatc agttctactg gtaggatcac
     1261 atggtacttt caagcatatc gaacaacaag acgtggcttt aattcaaaac gtgaggcgca
     1321 actcgcttat ctggaaatgg agaaacaaca aaggcttaag aaaaaaattg ttgcaagcaa
     1381 tgacaagttt agtgttgtag gtgagcaatg gttccaatat taccgttcgt taaatgaaca
     1441 aaaggaaata acctatgata agcgagagga acaattaaaa attattgttc gatggattgg
     1501 tgataaaaag ttgtctgatt tgacaccaga gtgccttcaa gaattgcttt ttaccttaaa
     1561 ggaatgtggc gttaatggaa caaatgttgg ctatgccaaa aatagtttac aatcacttat
     1621 tcaagttcta aatatgattt ttaaatattg tcaaagaaag aatttgataa gtgaaaatcc
     1681 aatgaagtct gtgagaatgc caaaatatca gttaacggta actgatttaa aagaagccat
     1741 aaataatatt gaggacaagt ttcttactgt cgatgaattg agaacttttt tgaattatgg
     1801 cattgttcat gaggaattac cgatgtctgt acttttccat gttctatttt acactggctg
     1861 ccgagtcagc gaggcgcttg ctctacagcc tggagatata gatttcaaaa gaaatgaaat
     1921 tctgttctat aagcaaacgg cagttaaagg taagagtaag gattttcgaa ttgaaactac
     1981 taagacagtt agttctgcta gacgagttct agtaactcct ctagttatgg aaaagctaca
     2041 acagttgatt aaggctttgg gtgagatgag gcaacattac aggtttatta tagaagaaac
     2101 atatttgttt gtgtatcttg attctagtaa acgtggtgtt ccttatcgtc gagagtatgt
     2161 aaatgatcac gtcaaacggt gcgtgaatcg ctcaggaatt aataagccat ttcatactca
     2221 tttagctcgt catacaatgg caagcttagt agcggaacac tgtagctggg aagtcttgaa
     2281 agatcgctta ggccatactg accaaacaac ttcgaaaatt tatcgccact taacgtcaaa
     2341 tgaaaaggta aaacctctga ttgcttttag ttctttagag agttaaaaaa acggtgaaag
     2401 cactggtatt acaggattct tgaaaaacga ttttttaaca aattagtcaa tatgtagtca
     2461 gaggtaataa aaatcctttt atatcaatac tttaacgact tttcacaagt atcaaagaaa
     2521 tccgtcgtta atcattgtta tcagactgag aaagaaccct tggtattcaa gggttctttt
     2581 ttcttag
//
LOCUS       DACDKM010000188         2505 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20834_2016
            SAMN12640370-rid9891663.denovo.191, whole genome shotgun sequence.
ACCESSION   DACDKM010000188 DACDKM010000000
VERSION     DACDKM010000188.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640370
            Sequence Read Archive: SRR10040891
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2505)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2505)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742835.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742835.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:04:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,012
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 29 of 75
            Pseudo Genes (incomplete)         :: 33 of 75
            Pseudo Genes (internal stop)      :: 19 of 75
            Pseudo Genes (multiple problems)  :: 5 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2505
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640370-rid9891663.denovo.191"
                     /isolate="E20834_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..701
                     /locus_tag="IV334_002755"
     CDS             <1..701
                     /locus_tag="IV334_002755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="rotamase"
                     /protein_id="HAP8944759.1"
                     /translation="VFKAFDDVYGKEISSKEVTKEYNEQIKKLGPNYKEQLKAVGQTE
                     ETYKKLFKQMLAFQYGLKANVKLTDKDLDTAWKEFYPEVSTQIILFSTKEEADKAKKE
                     ANEGENFSKLVQAYGKNKTLKETDGKMNFDSTNPEIPTEVKKAAFKLKNGEVSDIIPV
                     TDPTTYQQSYYLVKMVKKQDKGSNKDKYKSELEKIATEAKTLDTEFMDKTIRKVMKKD
                     NVTIKDPYVKNIFK"
     gene            complement(1285..1980)
                     /locus_tag="IV334_002756"
     CDS             complement(1285..1980)
                     /locus_tag="IV334_002756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HAP8944760.1"
                     /translation="MEIMLDTANTKSIYSCQQFLNLTGVTTNPTILKKEGASNFFSHM
                     KEIKKIIGQIPIHIQTIGRTEEEMIEDARVIIEELGKDTFIKVPVNQAGLQAIKKLKK
                     EGYLITGTAIYTELQGYLAINNGADYIAPYYNRMQNQGIDAMKVIDSLRTEIERTQRP
                     TKILAASFKNCEQVTTAIRSGAHAITLGKELLDQVLEMPSIDKAVRGFQEDWVQTFQA
                     ETIGELKKVADYF"
     gene            complement(1964..2362)
                     /locus_tag="IV334_002757"
     CDS             complement(1964..2362)
                     /locus_tag="IV334_002757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HAP8944761.1"
                     /translation="MTIFETVVTDVGIEAEVFRKEKIMILFGDEAPETLTDYCYKIEL
                     NQVRKEITCDMKLCFDKQMYVITAVGDVVQTNLNELGHITIKFDGSHEPELPGTMYVE
                     DKEMPHLRKGTIIKIDQEDQNGGKLYGNHA"
BASE COUNT          826 a          451 c          380 g          848 t
ORIGIN      
        1 ttgtcttcaa agcatttgat gatgtatacg gaaaagagat ttcttcaaag gaagtaacaa
       61 aagaatataa tgaacaaatc aaaaaactcg gtccaaacta caaggaacaa ttaaaagcag
      121 ttggccaaac agaagagaca tataagaaac tctttaaaca gatgctggct ttccaatacg
      181 gtttgaaagc caatgtaaaa ttaacagata aagatctaga cactgcttgg aaagaattct
      241 atccagaagt tagtacacag atcattctat tttcgactaa agaagaagca gataaagcaa
      301 aaaaagaagc aaatgaaggg gaaaatttct ccaagcttgt tcaagcttat ggaaaaaata
      361 aaacattaaa ggaaactgat ggaaaaatga attttgattc aaccaatcca gagattccta
      421 ctgaagtaaa aaaagcagca tttaaactaa aaaacgggga agtttctgat atcattcctg
      481 taacagatcc aacgacttat cagcaaagct attatctagt aaaaatggta aaaaaacagg
      541 ataaaggttc taataaagac aaatacaaat ctgagctaga aaaaattgct accgaagcaa
      601 aaactcttga tacggaattc atggataaaa caatccgtaa agtgatgaaa aaagacaatg
      661 taactattaa agatccttat gtaaaaaata tattcaaata aacaaatttg acccaattgg
      721 ggatgtggca aaatttgaga agtgattttt ggaaattcct tcttattttt gaggcaaacc
      781 acttttgcca caacttcatc ggggcctttt tttatttgta ccaaaacaaa tcactggatt
      841 tgttacaatc atgaggatat aatttaaaaa agttttttat ttctatcaca tgaatcaatg
      901 cgccattaag tagattcttt catgcttccc tattttttca cttgccgtat cgcttcaaaa
      961 acaactatca taaactcaaa ctcgactgaa aagttatttt aaaaaagact aaaaatatcc
     1021 cttctggact tgttcctctt attctgttat actagagaaa cacgaaagat atttttatgc
     1081 gacacttttc atttgttttt atgaaattta ttcaaaactg gcgtaacttc agagttacaa
     1141 ggatgtatta gaggttaggg aatacatcgt ttaaatgaga aataagtaat agtcacccaa
     1201 actttaaatt aatgaatatt gaatcgtcag atagcctcct cgagttttcg agaaggctat
     1261 cgtttatttt atggagaaat tgattcagaa gtaatcagcg acctttttta gttcaccaat
     1321 cgtttctgct tggaaagttt gcacccaatc ttcttggaag cccctcactg ctttatcaat
     1381 cgagggcatc tctaatactt gatctagtag ctctttacct aacgttatcg catgagctcc
     1441 agatcgaatg gctgtagtga cttgttcaca atttttgaaa ctagctgcta atatttttgt
     1501 tggtctttgc gtacgttcga tctcagtccg caacgaatca atcactttca tagcatcaat
     1561 gccttgattt tgcatcctgt tataataagg tgcaatataa tctgctccat tattaatagc
     1621 taaataccct tgtaattctg tgtaaatagc tgtaccggta atcaaataac cttctttttt
     1681 caactttttg atcgcttgaa ggcctgcttg attcaccggc accttgataa acgtatcttt
     1741 tcccagttct tctatgataa ctcgagcatc ctctatcatt tcttcttccg ttcttcctat
     1801 tgtctgaata tggataggaa tctgtccgat tatttttttg atttctttca tatgcgagaa
     1861 gaagtttgac gctccttctt ttttcagaat tgtaggattt gttgttacgc ctgttaaatt
     1921 caaaaactgt tgacaagaat aaatactttt tgtatttgct gtatcaagca tgatttccat
     1981 atagctttcc cccattctga tcctcttgat cgattttaat gattgtcccc tttcttaaat
     2041 gcggcatttc cttatcttct acatacattg ttcctggaag ttccggctca tgagatccat
     2101 caaattttat cgtgatatgt cctaattcat tcaaattagt ttggacaaca tctcctactg
     2161 ctgtgattac atacatttgt ttatcaaaac agagcttcat atcacaagtt atttcttttc
     2221 ttacctgatt caattcaatc ttataacaat aatctgttaa tgtttccggt gcttcatctc
     2281 caaataaaat cattattttt tctttcctga atacttctgc ttctatacca acatctgtta
     2341 caaccgtttc aaagatcgtc atctctgttc ctcctcgtaa aatatcattt gtctaatagt
     2401 ttttattcaa aatgtttcta gattataaaa tttattgaat aacacattcc taacggaatt
     2461 tgtttcgata ttgtgtgatt tctcagagta caatggatgt atgga
//
LOCUS       DACDKM010000189         2504 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20834_2016
            SAMN12640370-rid9891663.denovo.192, whole genome shotgun sequence.
ACCESSION   DACDKM010000189 DACDKM010000000
VERSION     DACDKM010000189.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640370
            Sequence Read Archive: SRR10040891
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2504)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2504)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742835.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742835.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:04:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,012
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 29 of 75
            Pseudo Genes (incomplete)         :: 33 of 75
            Pseudo Genes (internal stop)      :: 19 of 75
            Pseudo Genes (multiple problems)  :: 5 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2504
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640370-rid9891663.denovo.192"
                     /isolate="E20834_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(1034..1324)
                     /locus_tag="IV334_002758"
     CDS             complement(1034..1324)
                     /locus_tag="IV334_002758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8944762.1"
                     /translation="MEKKYGTVDKIRTLQVSARPLIRFSLNGVNCLIASHSLNFLAEV
                     DEGMKLVVTGYYNNRKQFVVRSYHVLGKPKIVVEYEKSLYPRKKVRQDERIN"
     gene            complement(1532..2224)
                     /locus_tag="IV334_002759"
     CDS             complement(1532..2224)
                     /locus_tag="IV334_002759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HAP8944763.1"
                     /translation="MKKKFITGGIVVAFLCLIGYTGTRYANEIILWGGETDIDTINEN
                     LTQLDHTLTDKEQALTDLTARLAQKEQTLQDSKADVQEYQTKVNELENQKNQSAAEKQ
                     NLESQLVSKDAQLQSKQNEINVKLDEINQKNDEINHLNADWTARLQEAQAKLSELQNT
                     VNGLNQQIATLTQEKTNVETQLTDTQKKLDEATAENSSLKQYIQKLEKAKDEVEDTAD
                     KSQQIVEEHLRK"
BASE COUNT          741 a          462 c          351 g          950 t
ORIGIN      
        1 acatttattt tctcaaaaac atacttgcaa agtaatacta caattcctaa cataatcgta
       61 gaatttaata atttccatag cataaagtgt ttagaaaaga acattaagaa tagttctata
      121 ataattcttg aactccatgt cttataatgt tctatcgaag cacccaaata gccaagttca
      181 tctaatctac ttgcataaac tacgtcatcc gctctcatag tcggcatata catattaaag
      241 agtaaaaaga taccccaaat ccctattagt aacaagatca gttctttgtt attcgttatt
      301 tttctattat gtttgttcat ttttatactc cttcttatat ttcctaatag agtataacat
      361 agctttaagc attacttaaa gataacttaa aagttatctt ttaaatactt aattgttaaa
      421 taccttattt gatgcaaaag aaatctacga tagtcaacaa aaaaattttc attatatcca
      481 gaaaaatttt tcttagttaa ggacctatgg attgcagtaa tgcagaattc gtcgaataaa
      541 ctgccaaaga catacaatca tattgctttt aaaattgacg aacaagatat agattcgttt
      601 gtctcaaaaa tccaaatgtt aggtttaact gtcgaaccag gacgttctcg tgtgaaaggc
      661 gaggcttctt caatttactt ttacgactat gataatcacc tcttcgagtt acataccggt
      721 actctccagg aacgtttaaa atcctacaat tccactacct gagactagta atgactagtc
      781 tctttttatg gcctctgatc cccccattga gcgaatacta atagggcttc gaattgatct
      841 gttcaatctt taagcaatat ccctcaaatg agtttggaaa gtttagtgat tcatcacaaa
      901 tacttaaata catgtagtta tatatagatt gaggttcttg tgagcgtatg ctcgtgtggg
      961 ttcaaatccc atcgtctgca tccatctttc caaaacaagc ggctaggaca tttttgtctc
     1021 agccgcttgt tttctagttt attctttcat cttgtcggac cttttttctt gggtatagac
     1081 ttttctcata ttctaccaca attttgggtt tccctaacac atgataggat ctcacaacaa
     1141 attgctttct attattataa taacctgtaa cgactaactt catcccttcg tctacttcag
     1201 ctaggaagtt caaactatgg gaagcaatca aacaattgac cccatttaaa ctgaaacgga
     1261 tcaacggtct ggcactaact tgcaaagtac ggatcttatc tactgttcca tattttttct
     1321 ccataacctt cacctcaata ccattatacg aacatacatt ccctttgtaa agaagaaact
     1381 ctgttttatc tttttgactt tttgtctatt acctttctta aaatcatatt ggcacttcct
     1441 gcgaagatga aggacatgta ctaaaaaaaa gaggcagcag tatagattac tgttgcttct
     1501 ttactaattt aggtttactt gttaagaaag attatttacg caaatgttct tcaacaattt
     1561 gctgagattt atcagctgta tcttccactt catcttttgc tttttctaac ttttgaatat
     1621 attgtttcaa gcttgagttt tcagcagttg cttcgtctaa tttcttttgt gtatctgtta
     1681 actgagtttc tacatttgtt ttttcttgtg tcagtgttgc gatttgttga ttcaaaccat
     1741 ttacagtatt ttgtaattct gaaagttttg cttgtgcttc ttgtagtctt gctgtccaat
     1801 ctgcattcag atgattgatt tcatcatttt tttggttgat ctcatctagt ttgacattga
     1861 tttcattttg tttgctttgt agttgtgcat ccttgctgac taattgactt tctaagtttt
     1921 gtttttctgc tgccgattga tttttttggt tttctaattc atttactttt gtttggtatt
     1981 cttggacatc tgcctttgaa tcttgcaatg tctgttcttt ttgtgctagt cgagcggtaa
     2041 gatctgtaag cgcttgctct ttatctgtca atgtatgatc aagctgtgtc agattctcat
     2101 tgattgtatc aatatctgtc tctccacccc acaaaataat ttcatttgcg taccgcgtgc
     2161 ctgtgtagcc aattagacat agaaatgcaa cgacaatccc accagtaata aatttctttt
     2221 tcatttgcat agctccttaa tccaatactt ttacatgcta aaagtaagtg atctcaaaca
     2281 caatgtcaag cgtttttaaa ttacatgtgt aattttaata taaatactat caaatactca
     2341 tttaatcctc cttttttatc tatttaaaaa atctttttta tagagaaaaa tgcaggttaa
     2401 tactttctta aataaatatt tatggtgaac aaaaacaaaa aagcaagttt tattaaacgt
     2461 aacccttttc atttcataga ttactgtatt tattacaaaa aacg
//
LOCUS       DACDKM010000190         2434 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20834_2016
            SAMN12640370-rid9891663.denovo.193, whole genome shotgun sequence.
ACCESSION   DACDKM010000190 DACDKM010000000
VERSION     DACDKM010000190.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640370
            Sequence Read Archive: SRR10040891
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2434)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2434)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742835.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742835.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:04:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,012
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 29 of 75
            Pseudo Genes (incomplete)         :: 33 of 75
            Pseudo Genes (internal stop)      :: 19 of 75
            Pseudo Genes (multiple problems)  :: 5 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2434
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640370-rid9891663.denovo.193"
                     /isolate="E20834_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            131..2284
                     /locus_tag="IV334_002760"
     CDS             131..2284
                     /locus_tag="IV334_002760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HAP8944764.1"
                     /translation="MMKDFMKKILNTSWLEKIRKGAQEPKNKTFRRSHVPFRLNFLFF
                     IIFTLFVALIARLGYLQIVNGEEMEARVKSTSTITVQESTPRGMIYDSTGKALVTNKA
                     NQAITYTRGTQVTAKDLLEIATKLNSLIDVPVDENLTDRDKKDFWLADPKHLKEATKR
                     LSAKEKQLGNSEQYSAMVNKVTADEINLNEEQLKIATIFKRMNSAYELNTVYIKNSGV
                     TDQELAVVAERASELPGVSTGTDWTREYNAASSLKSILGSVTTEKQGLPADEAEKYLA
                     KGYSRNDRVGQSYLEKQYEDVLQGTKTQYEVSLDNEGNVSNQKEIFSGEKGSNLMLSM
                     NAEFQSKVEEILKRNYQTLINNGKAQYSPGAYAVAMNPQTGEVLAMTGFSHEQGSKEI
                     TENALGTITSAFDPGSVVKAGTLTAGWASNAISGNQVLIDEPIRLQGASEKSSVFNRS
                     GQVALDAVKASELSSNTYMIKVALKMLGLDYTPGMGLPSLDEEAKAYQQLRDSFKEFG
                     LGTTTGIDLPNESPGISRSVDYMKKFNADNGKEWYAPGNFTDLAFGQFDTYTPIQLAQ
                     YASTVANGGKRVQPHLVKAIYGNDENGNLGEVKKEIGTTVENTVNISAENMAILREGF
                     HQVVHGTDPYTTAKPLASAKMDLSAKTGTAETVAEGHPDIATVNSNIVAYGPTDNPEI
                     AISVVLPNLLDEQDHINLTIAKEIMDAYYDMFMAK"
BASE COUNT          832 a          427 c          538 g          637 t
ORIGIN      
        1 ctgtgaaaca aaaagttgat tttatcttga aattaggtaa agaatttctt gctccgccca
       61 ttttttcttt tttattaaga agtttttggg tacaatgaag catgaatgta aataggcgga
      121 aggataggaa atgatgaaag attttatgaa aaaaatatta aatacttctt ggttagaaaa
      181 aataagaaaa ggtgcacaag aacctaagaa caaaacgttt cgccggtccc atgttccttt
      241 tcgcttaaat tttctttttt tcattatatt tacgttattt gttgcgttga ttgctagatt
      301 gggttatttg cagatcgtga acggcgaaga gatggaggcg cgagtgaagt ctacttctac
      361 gattacggtc caagaaagta ctcctcgcgg aatgatctac gactccacag gaaaagcttt
      421 agttacgaat aaagcgaatc aggccatcac atatacgaga ggaacacaag ttacggctaa
      481 agatttgctt gaaatcgcaa cgaagctgaa ctcgctgatc gatgttccgg tagatgaaaa
      541 tctgacagat agagataaaa aagatttttg gctggctgat cccaaacatt tgaaagaagc
      601 cacaaaaaga ctttcggcaa aagaaaagca attagggaac agcgaacaat atagtgcgat
      661 ggtcaataaa gtcacagctg atgagattaa tttaaacgag gagcaattaa aaattgctac
      721 catctttaaa cggatgaata gtgcatatga attaaacact gtctatatca aaaactctgg
      781 tgttactgat caagagttag ctgttgtggc agaacgcgct tctgaacttc caggagtttc
      841 gactggcaca gactggacac gagaatataa tgcagcaagt tcactgaaaa gtattcttgg
      901 cagtgtgacg actgaaaagc aaggacttcc agcagatgaa gcagagaaat acctagcaaa
      961 aggttattct cggaatgacc gtgtaggaca aagttatttg gaaaaacaat atgaagatgt
     1021 cctgcaagga accaaaaccc aatatgaagt ttcccttgat aatgaaggaa atgtttcgaa
     1081 tcaaaaagaa atcttctctg gggaaaaagg ttctaattta atgttatcta tgaatgcaga
     1141 attccaatca aaagtagaag aaattttgaa aaggaattat caaacattga tcaacaacgg
     1201 caaagcacag tattcgcctg gtgcttatgc agtggctatg aatcctcaga caggagaagt
     1261 gctagcaatg actggtttca gtcatgaaca aggttccaaa gaaatcaccg agaatgcttt
     1321 aggcacgatt actagcgcct ttgatccagg gtctgtagtc aaggctggta ctttgacagc
     1381 aggctgggca agtaacgcga tcagcggaaa tcaagttttg attgatgagc cgattcgtct
     1441 gcaaggtgct tcagaaaaaa gctctgtctt taatcgaagc ggacaagttg ctcttgatgc
     1501 agtcaaagca tcggaattgt cttctaatac gtatatgatc aaagtggcgt taaagatgtt
     1561 aggactagac tatacacctg gaatgggact acctagttta gatgaagaag ctaaagctta
     1621 tcagcaattg agagactctt tcaaagagtt tggtcttggt acaacgactg ggatcgattt
     1681 gccgaatgaa tctcctggga tctcacgttc tgttgattat atgaagaagt tcaatgcgga
     1741 taatggcaaa gaatggtatg ctccaggtaa ctttaccgat ttggcatttg gacagtttga
     1801 cacatataca ccaatccaat tagctcaata tgcttcaaca gtcgctaatg gcgggaaacg
     1861 agtacagccg cacctagtca aagcgatcta tggcaatgac gaaaatggaa acttgggtga
     1921 agtgaagaaa gaaattggca caacagtaga aaatacggtc aatatttctg cagaaaatat
     1981 ggcgatcctc cgtgaaggat tccaccaagt tgtccatggt accgatcctt acacaacagc
     2041 caaaccatta gccagtgcga aaatggacct ttccgcaaaa acagggactg cagaaacggt
     2101 tgcggaaggt catccagata ttgcgacagt caatagtaat atcgtggcat acggaccaac
     2161 agacaatccg gaaattgcca tcagtgttgt cttgccgaac ttattagacg aacaagacca
     2221 cataaacttg acgattgcta aagaaataat ggacgcgtat tatgatatgt tcatggcaaa
     2281 ataaaaaaga ggtcgtgaaa aagccgctta gtttcgagta tgaagaaaaa ttttgtgaaa
     2341 atagcttctc acatttttgc ttaaatttga cttcataccg agaaactggc ttttgaacac
     2401 cgtttactcg gcaaaaaagg ttgtgacaaa aatt
//
LOCUS       DACDKM010000191         2407 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20834_2016
            SAMN12640370-rid9891663.denovo.194, whole genome shotgun sequence.
ACCESSION   DACDKM010000191 DACDKM010000000
VERSION     DACDKM010000191.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640370
            Sequence Read Archive: SRR10040891
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2407)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2407)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742835.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742835.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:04:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,012
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 29 of 75
            Pseudo Genes (incomplete)         :: 33 of 75
            Pseudo Genes (internal stop)      :: 19 of 75
            Pseudo Genes (multiple problems)  :: 5 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2407
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640370-rid9891663.denovo.194"
                     /isolate="E20834_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            5..1150
                     /locus_tag="IV334_002761"
     CDS             5..1150
                     /locus_tag="IV334_002761"
                     /EC_number="2.5.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
                     /protein_id="HAP8944765.1"
                     /translation="MHIQTKLIHGGISEDPTTGAVSVPIYQTSTYRQDGVGQPKQYEY
                     SRSGNPTRFALEELIADLEGGVRGFAFSSGLSGIHAVFSLFQAGDHILLGDDVYGGTF
                     RLFDKVLTKNGLEYTIIDTSNLDKIEQSIKPNTKALYLETPSNPLLKITDLEKSATLA
                     HQHGLIVIADNTFATPYFQRPLDLGSDIVVHSGTKYLGGHSDVVAGLVTSNHKDLADQ
                     IGFYQNAIGAVLGPQDSWLLQRGIKTLSVRMEEHQKNAFVVADFLFSHPAVEKVYYPG
                     LPDHELHGVAKQQMSGFSGMISFTLKNEESAIPFVESLQLFTLAESLGGVESLVEIPS
                     VMTHASIPKEKREEAGIKDGLIRLSVGIEYGQDLINDLAQAFDRIKN"
     gene            1321..1779
                     /locus_tag="IV334_002762"
     CDS             1321..1779
                     /locus_tag="IV334_002762"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HAP8944766.1"
                     /translation="MRMNVESFNLDHTKVKAPYVRVADRKTGAHGDVIIKYDVRFKQP
                     NKEHMDMPSLHSLEHLTAELIRNHADYIVDWSPMGCQTGFYLTVLNHESYEDILDVLE
                     ATMKDVLEATEVPASNETQCGWAASHTLEGAQALAREFLAKRDEWSEVEA"
     gene            1938..>2407
                     /locus_tag="IV334_002763"
     CDS             1938..>2407
                     /locus_tag="IV334_002763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HAP8944767.1"
                     /translation="MSIYNFETTLEDGTVYSLKKYAGKPIIIVNTATKCGLAPQFKQL
                     EELYQAYQTQGLVILGFPSNQFKQELDSATEAAEACKTTYGVTFPMHQLIDVNGENTD
                     PLFTYLKNEAPGTVTKAIKWNFTKFLIDRNGKVIERFAPQTSPLKMTAAIESVL"
BASE COUNT          802 a          452 c          455 g          698 t
ORIGIN      
        1 aaacatgcac attcaaacaa aattgatcca cggaggcatc agtgaagatc ccacaacagg
       61 agctgttagt gtacctattt atcaaacatc cacttatcgg caagatggcg tcggccagcc
      121 taaacaatat gaatattcaa gatctggtaa tcctactcgt tttgcattgg aagaactgat
      181 tgctgatttg gaaggcggtg ttcgaggatt cgctttttct tctggtcttt ctgggattca
      241 tgccgttttt tctctttttc aagctggcga tcatatctta ttaggagatg atgtctatgg
      301 aggaacattc cgtctatttg acaaagttct tactaaaaat ggtctcgagt acacgatcat
      361 agatacgagt aatcttgata aaatagaaca atccatcaaa cccaatacaa aagcacttta
      421 tcttgaaaca cctagcaatc cgcttctaaa gattactgat ttagaaaaaa gtgccactct
      481 ggctcatcaa catggattga tagttattgc tgacaataca tttgctactc cttattttca
      541 aagaccttta gatctcggtt ctgatatcgt tgtccatagc ggtaccaaat atctaggcgg
      601 acacagcgat gtagtagctg gtttagtgac ttctaaccat aaagatctag ccgatcagat
      661 cggattttac caaaatgcta tcggagccgt acttggtccc caagatagct ggttacttca
      721 gcgcggaatc aagacactaa gtgttcgtat ggaggaacat caaaaaaatg cttttgtggt
      781 agctgatttt ctattttctc atccagcagt agaaaaggtc tactatccag gattacccga
      841 tcatgaactc catggagttg ctaagcagca gatgagcggc tttagcggaa tgatttcttt
      901 tactttaaaa aatgaagaat ctgctattcc atttgttgaa tcactccaac tatttacttt
      961 agcagaaagt ttaggtggcg tagaaagttt agtggaaatt ccttctgtca tgactcatgc
     1021 gtctattcca aaagaaaaac gagaagaggc tggcataaag gacggcttga tccgtctttc
     1081 tgtaggaatt gagtatggtc aggatctcat aaacgattta gctcaggcat ttgacagaat
     1141 caagaactag aagtcccaat aaacgactta gcagaaaaat ctaaaaaata tttcagattg
     1201 ctacaccttg gaaacgataa gtgttagaat caagtagaaa gacgtttttg taaatttcac
     1261 taaataattc tttagtgaaa agcatcaaaa cgaatgaaca aacagatagg agtcttacaa
     1321 atgagaatga atgtcgaaag ttttaatctt gaccatacaa aagtcaaagc accttatgta
     1381 cgtgtagctg atcgaaaaac gggtgcgcat ggcgatgtca tcattaaata cgacgtccgc
     1441 tttaaacaac caaataaaga acacatggac atgccaagtc tacattcact tgagcacttg
     1501 actgccgaac taatccgcaa tcacgcagat tacatcgtag actggtctcc aatgggatgc
     1561 caaacaggct tttacttgac tgtattgaat catgaaagct acgaagacat tttagatgtt
     1621 cttgaagcaa caatgaaaga tgttcttgaa gctactgaag tacctgcaag caatgaaaca
     1681 caatgtggct gggctgccag ccatacacta gaaggtgcac aagctttggc tcgtgaattt
     1741 ttagcaaaac gcgatgaatg gtctgaagta gaagcatagt ttctaaaaga acgtaaagat
     1801 gagaagcaat atagctatct tatcttcgta ttttattaaa tatttattgt ctgaaaagag
     1861 ttctaaacgg gaaaacaaat agctagtttt cagtttagcg ctctttttat tatataaaat
     1921 agaaaggaga aaactttatg tctatttata attttgaaac tactttagaa gacggcactg
     1981 tctattcatt gaaaaaatat gcaggcaaac cgataatcat tgttaacact gcaacaaaat
     2041 gcggtctagc acctcaattt aaacaattag aagaacttta ccaagcttat cagacacaag
     2101 gtctggttat cttaggcttt ccttccaatc aatttaaaca agagctagat tcagcgactg
     2161 aagcagcaga agcttgtaaa accacttatg gcgttacctt ccctatgcat caattgattg
     2221 acgtaaatgg agaaaataca gatcctcttt ttacttatct taaaaatgaa gctcctggta
     2281 ctgtcactaa agcaataaaa tggaatttta ctaaattttt gattgataga aatggcaaag
     2341 taatcgaacg atttgcacca caaacttctc cattgaaaat gaccgctgca attgaatccg
     2401 ttcttta
//
LOCUS       DACDKM010000192         2400 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20834_2016
            SAMN12640370-rid9891663.denovo.195, whole genome shotgun sequence.
ACCESSION   DACDKM010000192 DACDKM010000000
VERSION     DACDKM010000192.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640370
            Sequence Read Archive: SRR10040891
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2400)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2400)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742835.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742835.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:04:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,012
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 29 of 75
            Pseudo Genes (incomplete)         :: 33 of 75
            Pseudo Genes (internal stop)      :: 19 of 75
            Pseudo Genes (multiple problems)  :: 5 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2400
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640370-rid9891663.denovo.195"
                     /isolate="E20834_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            207..2069
                     /locus_tag="IV334_002764"
     CDS             207..2069
                     /locus_tag="IV334_002764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAP8944768.1"
                     /translation="MQQLTKRERQLLIYLVEMSEPVTIKKLAEKENVSVRTIKYDLDD
                     IREWLNERKQVLYSKRSQGIWMNISSSDRIKLKSELMDVNRFEIFADQSLRIKRLLIM
                     LLVTKEAITGMKMANALDVSKDTIMNDLDVLENNFLKEGLTLNRQARKGFWIIGEERL
                     VRLTIEEILQKEFTDYDIYKLMSLLLNGGETEYFEMYSATATPIQEVFNQVIIRMRHL
                     LEFENLEKLNYAELLNILIRVTIATVRLRKEATIGRYQLVAEQELDKNDLSYRVMQQV
                     FDYYQLPLFEDEYHYIYSDTFDTIHQQDVMKLVENVIYKVSKAINHSFYQDPQLLTNL
                     YAHLSMRLSRKKKFVNEYNPFKEDIKQKYPELFDAIKQAVRQEISEQNILINDSFVAY
                     IALHFLVSYEREEDLRSVRAVYICSTGLGVTSLIKQKINEELNNITIVAFASVLNAQE
                     MIATKDPDLVISIFPINGIDRPFIKVHPLPTEQDLKKIQQIIKKILASSNQQERHNLN
                     LRETSINKDTLEDFSKELILKAYTIYERLLVILEAELIQEYREAFLLHVFLMVHRITF
                     DQQYMAEGRQVDQIILDKGTLVGKIEAVFAENNLGINQSEISALFSYIKEGASS"
     gene            2069..>2400
                     /locus_tag="IV334_002765"
     CDS             2069..>2400
                     /locus_tag="IV334_002765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAP8944769.1"
                     /translation="MKMSDEALKIIEESQNREKLKQVIDLIEERLVEHAIVPTELQWT
                     ILINHLNEMLKRSQRKETIPVVDRDLFKEVSKEAITISDEIVRSIGNLTDDEIYVLSI
                     HFETAKNN"
BASE COUNT          866 a          353 c          466 g          715 t
ORIGIN      
        1 aaaaatttga aaaagcgttt gattatttat attacgactt agtgcaataa aaaacgcttt
       61 ttaacagtac atgtagtcgt ctttataata gataaactct gtaatattag ttaaaaagct
      121 ataagttgca cttagttaag tgcaaacatt tcatattgtc gttagtttag aaaacgctta
      181 tattagcaat gtaaggagag atgataatgc agcagctaac aaaaagagaa aggcagcttt
      241 tgatttactt ggtggaaatg tctgaaccgg taacaatcaa aaagttggct gaaaaagaaa
      301 atgttagtgt tcggacgata aaatacgatc tagatgatat aagagagtgg ctgaatgagc
      361 ggaagcaagt cctatatagc aaacgtagtc agggaatttg gatgaatatt agtagctctg
      421 atcgaatcaa attaaaaagc gaattgatgg atgttaatcg ttttgaaata tttgcagatc
      481 aaagtctccg aatcaaacgt ttactaatta tgcttttagt aacaaaagaa gctattactg
      541 gaatgaaaat ggccaacgca ttagatgtca gcaaagatac gatcatgaat gatttagatg
      601 tgctggaaaa taattttctt aaggaaggat tgaccttgaa tcgtcaggcg cgaaagggtt
      661 tttggatcat aggagaagaa cgtctagttc gattaacgat tgaagagatt cttcaaaaag
      721 agttcacgga ttatgacatt tataaattaa tgtcattgtt attgaatggc ggtgagactg
      781 aatactttga aatgtattct gctactgcta cacctatcca agaggtattt aatcaagtaa
      841 tcattcgaat gcgacactta ctggaatttg aaaatctaga gaaactgaat tacgcggaat
      901 tactaaatat tctgattcga gtgacaattg ctaccgttcg cttacgcaaa gaggctacga
      961 ttggtcgtta tcaattggtt gctgaacaag aattagacaa aaacgatctt tcttatcgcg
     1021 tcatgcagca ggtctttgat tattatcagt tgccactgtt cgaggatgaa tatcattata
     1081 tctatagtga tacattcgac acgattcacc agcaagacgt catgaaacta gttgagaatg
     1141 tgatttataa agtaagtaaa gcaatcaacc attcttttta tcaagatcca caattgttga
     1201 cgaatctgta tgctcattta tccatgcgat tatcgagaaa gaaaaaattt gtcaatgaat
     1261 ataatccttt taaagaagat atcaagcaga aatacccgga attatttgat gcgatcaagc
     1321 aagcagttcg acaagaaata agtgaacaaa atattctgat caatgattct ttcgtggcat
     1381 atattgcctt acactttttg gtttcctacg aaagagaaga agatctacga agcgttagag
     1441 ccgtctacat ttgttcaaca gggttgggtg tgactagtct gatcaagcaa aagatcaatg
     1501 aagaattaaa taatatcact atcgtggcat ttgcttcagt attgaatgcg caagaaatga
     1561 ttgctaccaa agatcctgat ttggtgatta gtatttttcc aattaatggc atcgatcgtc
     1621 ctttcatcaa agtccatcca ttacccactg aacaggactt gaaaaaaatt caacaaataa
     1681 taaaaaaaat acttgcttct tccaatcagc aggaaaggca taacttgaat cttagagaaa
     1741 cgtccatcaa taaagatact ttagaagact ttagcaagga attgattttg aaagcttata
     1801 cgatttatga acgtttactt gtcattttgg aagcagaatt gatccaagaa tatcgagaag
     1861 cttttttgct tcatgtattc ttaatggttc atcgtatcac atttgatcaa caatatatgg
     1921 cagaaggtcg tcaagtagac cagattattt tagacaaagg aacactggta ggaaaaatcg
     1981 aagcagtttt tgcagaaaat aatttaggaa tcaatcagtc agaaatcagt gcattattct
     2041 catatataaa agaaggagcg tcaagttagt gaaaatgtca gacgaggctt taaaaattat
     2101 cgaggaaagt caaaatcggg aaaagttaaa acaagtgatt gatctaattg aagagcgatt
     2161 agtagaacat gctattgtac caaccgagtt gcagtggacc attctaatta atcacttgaa
     2221 tgagatgctg aaacgttctc aaagaaagga aacgattccc gtagtcgatc gggatttgtt
     2281 caaagaagta tccaaagaag ccataactat ttctgatgaa atagttagaa gtatcggtaa
     2341 tttaaccgat gatgaaatat atgttttgtc gattcatttt gaaacagcaa aaaataatta
//
LOCUS       DACDKM010000193         2398 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20834_2016
            SAMN12640370-rid9891663.denovo.196, whole genome shotgun sequence.
ACCESSION   DACDKM010000193 DACDKM010000000
VERSION     DACDKM010000193.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640370
            Sequence Read Archive: SRR10040891
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2398)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2398)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742835.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742835.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:04:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,012
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 29 of 75
            Pseudo Genes (incomplete)         :: 33 of 75
            Pseudo Genes (internal stop)      :: 19 of 75
            Pseudo Genes (multiple problems)  :: 5 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2398
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640370-rid9891663.denovo.196"
                     /isolate="E20834_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(563..1120)
                     /locus_tag="IV334_002766"
     CDS             complement(563..1120)
                     /locus_tag="IV334_002766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8944770.1"
                     /translation="MRNLSNRNKILIIIVVIAVFHLGTNAVLSRIILGPKPPRPEITR
                     GEFDFRLEYEVDGERIVIEDTIVALFDGFSADAGSMAWYRTWRLHLASDRRSRNILLD
                     ELEDGRRIYYVPESANYSMGDVQKKREPNPNWYPFNGVTIEYPRNKTPEIGAKFISGL
                     EDLYDRFGIRLISWEHDPPIENRFE"
     gene            complement(1299..1910)
                     /locus_tag="IV334_002767"
     CDS             complement(1299..1910)
                     /locus_tag="IV334_002767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8944771.1"
                     /translation="MNSNRGNKMEYNGSMRIVQIIFWITSGIVIIGGVFLMLPSLIFP
                     FFALISPKIPEPEITYGEFPFRIEYELDGQINIIEDTVIAEFNGCEFSAGSMKRERRW
                     RSRLASGREDLPFGDDLGIYFSRGSAQYYMGENVQSMSLKPHIALRNLEEGFKREVDF
                     ILGESGYIRTVLNFGEAQEVLTQYGITLINWEISEPIVNNFGD"
     gene            complement(2023..2280)
                     /locus_tag="IV334_002768"
     CDS             complement(2023..2280)
                     /locus_tag="IV334_002768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8944772.1"
                     /translation="MRAVHVNPTIEITMIGHSKGGAEAANSVAINTNAIIFNPATVNL
                     SAYELDSNTYTANMVAYVVEGDALNVVFGGVYTYWRINHVR"
BASE COUNT          701 a          516 c          318 g          863 t
ORIGIN      
        1 taaatggtgt ccaaaagctt gcatctgcca gattatctca tttcttatga caagctaagc
       61 aaattatctc caccaaataa tttattcata tttttgactt gcgcatacta actaattctt
      121 aaattttcat acaaaatttc tcaatttccc ttaacgagta atatttatgt acggtgattc
      181 tcactagtat cccaatcact ttttaagtta tcccgatttc aattctactt caaaaatagt
      241 tgcttttatg tatgctgtta gttgattaga tggtcatttg ttgagctagg tcgtttattt
      301 taaacaagtt gtttctttag cttataccta tctcgtctac caaacgcaag atggcaaaca
      361 caaatgcata cgcatctgta tttgccactt tgattcgtat aaagctaaca actattaact
      421 cctctcagct aatagttgct ctctttttta ttcagattca tttttgctga tcacagccta
      481 gtatttcaat ggttttgaac actaaaccaa attgttccat tacaacattc actggctgat
      541 gaaatggatt agtagatttc gattattcaa agcgattttc tattggtgga tcgtgttccc
      601 aactaataag acggattcca aacctatcgt acagatcttc taaaccactt ataaattttg
      661 cacctatttc aggtgtttta ttgcgtggat attctattgt tacgccatta aatggatacc
      721 aattaggatt aggctctctt tttttctgta catctcccat agaatagtta gcactttctg
      781 ggacataata tattcttcga ccatcctcta attcatctaa taagatattt cgtgatcgtc
      841 tatcacttgc taaatgtaga cgccacgtgc gataccatgc catgctacct gcatctgcag
      901 aaaatccatc aaataatgca acaattgtat cttctatcac aatcctttcc ccatctactt
      961 catattctaa tctaaaatca aactcccctc gggtaatttc tggtcttggt ggcttaggac
     1021 ccaatattat tctacttaaa accgcattcg ttcccagatg aaacacagct ataacaacaa
     1081 taatgattaa tattttgttt ctatttgata aatttctcat tattcgcaca tcacctctac
     1141 attctcacat attagttcgt aatactcctt taatatcaaa atatctactg gtcgtaataa
     1201 tattcgctat gaattcctac tactcgtata agaaaggcca aatcatcagc ttttacttgc
     1261 tgatgatttg gcttgtttct atttatcgta attgttttct aatcaccaaa attgttaaca
     1321 atcggttcag atatttccca gttgattaag gtaattccgt attgtgtcaa aacttcttgc
     1381 gcttctccga aattaagtac agttcttatg tatccacttt ctcctaatat aaagtctacc
     1441 tctcttttaa aaccttcctc gagatttcta agggctatat gtggttttaa tgacattgat
     1501 tgtacatttt cccccatata atattgcgca cttcctcgac taaaatagat tcctagatcg
     1561 tccccaaaag gtagatcttc tctaccacta gctaatctac ttctccatct tcgttctctt
     1621 ttcatagaac ctgcgctgaa ctcacacccg ttaaactcag ctatgacagt atcttcaatt
     1681 atgttaatct gtccatctag ttcgtattca atcctaaatg gaaattctcc ataggtgatt
     1741 tccggttcgg gtattttagg tgagattaat gcaaagaaag gaaatattaa gcttggcaac
     1801 atcaaaaaaa ctcctcctat gatcacaatt cccgatgtaa tccaaaatat aatttgtaca
     1861 attctcattg atccattgta ttccatttta ttccccctat tactattcat atccgcctat
     1921 ctatatcgat cttccaatgc agagataatt gcttccatac cttgattttt tactgagtta
     1981 cctatgcgag atatcattct ccactaaggt gcatcatatt gtctatctaa catgattaat
     2041 tctccaatag gtgtatactc ctccaaaaac aacatttaat gcatctccct cgacaacata
     2101 agctaccatg tttgctgtat atgtgtttga gtctaattca taagcagata aatttactgt
     2161 cgctggatta aatataatcg cattcgtatt tatagctact gaatttgctg cttcagcacc
     2221 accttttgaa tgtccgatca ttgtgatttc aatagtagga tttacgtgga cagccctcac
     2281 cgtttaccct gaccattata gaaatcgctt acttttttga actacgcaac aaaggataca
     2341 atgctcctaa cttcactgtc tccatttttg tccactaagg aatataaaaa ataggcat
//
LOCUS       DACDKM010000194         2325 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20834_2016
            SAMN12640370-rid9891663.denovo.197, whole genome shotgun sequence.
ACCESSION   DACDKM010000194 DACDKM010000000
VERSION     DACDKM010000194.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640370
            Sequence Read Archive: SRR10040891
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2325)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2325)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742835.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742835.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:04:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,012
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 29 of 75
            Pseudo Genes (incomplete)         :: 33 of 75
            Pseudo Genes (internal stop)      :: 19 of 75
            Pseudo Genes (multiple problems)  :: 5 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2325
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640370-rid9891663.denovo.197"
                     /isolate="E20834_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            446..1939
                     /locus_tag="IV334_002769"
     CDS             446..1939
                     /locus_tag="IV334_002769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000947690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein RepR"
                     /protein_id="HAP8944773.1"
                     /translation="MLSEIYSAILKNGLRTYKYRNSTMKPAGHIDSSRSGAIFAYRSK
                     QLMNVGRGMVITSEEAILENEKKLTHWTPNTYRFGTYADDYRTVVKGHSEKNLSQINT
                     FVVDIDSKENHQGEIILACLDQVGYMPTLILESDHGYQVYFVLKTPGYVTKKSNFKVI
                     DVAKKISKTIRLQLAEKLSGVDLGCNHFGIARFPQENNIVFKEMNYQYSFADWIQWSM
                     KLESDQKTKEDRERKLIVFPEKKEIRQVDEPWFDMLLHKANIVGGEGRLGRNNVIFTL
                     SLAYYSSGYALDTCEYNMFTFNERLQEPLSEAELVKIVRSAYSGNYQAASREFIIELC
                     QQWVSPTLKNNDLFIQRRGWWKFKKPRDQRKYSHKNEWKEDLLAYLSEKSLASEPYLN
                     LSKKELASQLTMPKRTLDKILNELREENKVFYKVTRGRNGGMIIASVKTLFARVIQLS
                     KEKRLDYSTRITEVFGLNESLVEETFRQLSTPLNPLKEVNLFEVDTG"
     gene            2074..>2325
                     /locus_tag="IV334_002770"
     CDS             2074..>2325
                     /locus_tag="IV334_002770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000053907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein PrgN"
                     /protein_id="HAP8944774.1"
                     /translation="MSLKNYVYSHPVNVFIIGRLGLSVEVFCELYGFKQGTLSSWVTR
                     EKTVASLPIEFLHGLSLASGETMDQVYDCLCVLEQEYIEY"
BASE COUNT          789 a          365 c          475 g          696 t
ORIGIN      
        1 tgtcaaaata tatttgtgcg ttttgcgttg acaatttaga gtgaaaagag gtaaacttaa
       61 ttctatagaa accacagata attcactatt tacaaagtag caaaagcagt ggtactcttt
      121 aaacagaaaa attacataaa aaaaagagac ggaaataatt gattttagca gaatcaatta
      181 tttctgaatc acatggttag gacatgtgtt cacgctcgtc tcaagaatat gtaagtagct
      241 accaactact tatattaaaa gttgatcgga taccttttcc gattgccttt attctaccaa
      301 aaaaaggcga gcaactcaac agaagaacgt ttatttagtg tacttaaatt aaatattctt
      361 aaacactgaa gagagaacac atactatggc tgagattaaa gctaaactgt gttctctttt
      421 tgtttgagaa aggacgttac aatcgatgtt gagcgaaatt tattcagcta ttcttaaaaa
      481 cggcttacgt acatacaaat atcgaaacag caccatgaaa cctgcaggtc atattgattc
      541 cagtaggagt ggtgctatct ttgcgtaccg tagcaagcaa ttaatgaatg ttgggcgtgg
      601 tatggtcatt acttcggaag aagctatttt agagaacgaa aagaaactaa cgcattggac
      661 acctaatacc tatcgctttg gtacttatgc ggatgattat cgaacagtgg ttaaaggtca
      721 ttctgagaaa aacttaagtc aaatcaacac atttgtagtt gatatcgatt caaaagagaa
      781 ccatcaaggc gaaattattt tagcctgttt agatcaagtg ggctacatgc caacgctaat
      841 tttagaatca gatcatggtt atcaagttta ttttgtacta aagacaccag gctatgtcac
      901 aaagaaaagc aatttcaagg tgatcgatgt agcaaaaaaa atttcaaaaa caattcgtct
      961 acagttggct gaaaaattgt ccggagtgga tctaggatgt aaccacttcg gaattgctcg
     1021 attcccacaa gaaaataata ttgtttttaa agaaatgaac tatcagtatt cttttgctga
     1081 ctggattcaa tggtcgatga agcttgaatc agatcaaaaa acaaaagaag atcgagaacg
     1141 aaaactaatt gtctttcctg agaaaaaaga aattcgtcaa gtagatgaac cgtggttcga
     1201 tatgttactt cataaagcaa acatcgtcgg cggagaagga cgtcttggtc gaaataatgt
     1261 gattttcacg ctttctttag cttattactc ttcaggttat gcattggata cttgcgaata
     1321 caatatgttc acattcaacg aacggttaca ggaaccgcta tctgaagcag aattagtaaa
     1381 gattgtacgt agtgcttatt ctggtaatta tcaagcagca agcagagaat tcattattga
     1441 attgtgccag caatgggttt caccaacctt aaaaaataat gatctgttca ttcaacgacg
     1501 tggctggtgg aaatttaaaa agccacgaga tcaaagaaaa tatagtcata aaaatgaatg
     1561 gaaagaagat ctattggctt atctaagcga aaaaagtctt gctagtgagc cttatttgaa
     1621 cctgtcaaaa aaagaattgg cttcacaact aacgatgccc aagcgaacac ttgataagat
     1681 cttaaatgag ttacgagagg agaataaagt cttctacaag gtaacacgag gaagaaatgg
     1741 cggaatgatc attgcttcag tcaaaacgtt gttcgcgagg gttattcaac taagtaaaga
     1801 aaagagacta gattattcaa caaggatcac agaggtattt ggactcaacg agagcttagt
     1861 agaagagaca tttagacaat tatcaacccc cttgaaccct cttaaagaag tgaatctttt
     1921 tgaagtagat acaggttgaa aaataaaagg tgcaaattgc cttatacaat atatctatga
     1981 tacttctgta ggtgttgctc cctttgccta gctacgtgta ggtggtatac ttataactgc
     2041 gtatgcagtc ttatgtatca tggaggaagt tgtatgtcgt taaaaaatta tgtctattca
     2101 catcctgtta atgtgtttat cattggtcgt ttgggattat ctgtggaagt gttctgtgag
     2161 ctttatggtt ttaagcaggg gactttgtct tcttgggtta ccagggaaaa aacagttgct
     2221 tctttgccga ttgagtttct tcatgggttg tccttagctt ctggggagac tatggatcag
     2281 gtatatgatt gtttgtgtgt gttagagcag gagtatattg agtat
//
LOCUS       DACDKM010000195         2297 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20834_2016
            SAMN12640370-rid9891663.denovo.198, whole genome shotgun sequence.
ACCESSION   DACDKM010000195 DACDKM010000000
VERSION     DACDKM010000195.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640370
            Sequence Read Archive: SRR10040891
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2297)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2297)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742835.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742835.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:04:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,012
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 29 of 75
            Pseudo Genes (incomplete)         :: 33 of 75
            Pseudo Genes (internal stop)      :: 19 of 75
            Pseudo Genes (multiple problems)  :: 5 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2297
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640370-rid9891663.denovo.198"
                     /isolate="E20834_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(243..464)
                     /locus_tag="IV334_002771"
     CDS             complement(243..464)
                     /locus_tag="IV334_002771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8944775.1"
                     /translation="MEEEKIFEKRWELASVEQRARYHNLMSSYRNIDWTYKEKKYLLW
                     LCQLDVNTFETFEVILDKIKNSNEKRADL"
     gene            complement(562..807)
                     /locus_tag="IV334_002772"
     CDS             complement(562..807)
                     /locus_tag="IV334_002772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008271463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8944776.1"
                     /translation="MDFPEVVKEVQEIGATISDSRSSPMNYQDAHGIKIIDQERNVRK
                     YTTINECVINENLCRAPITKHIKNRSKAKDGRTFTTF"
     gene            1047..1661
                     /locus_tag="IV334_002773"
                     /pseudo
     CDS             1047..1661
                     /locus_tag="IV334_002773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337511.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
     gene            complement(2031..>2297)
                     /locus_tag="IV334_002774"
     CDS             complement(2031..>2297)
                     /locus_tag="IV334_002774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4062 domain-containing protein"
                     /protein_id="HAP8944777.1"
                     /translation="SDNNLRSYLSDRVFDVLEKLSKSGILKEEYDSSFDEYPSRYILI
                     SEDYKKFKRYFLKNEKKISVIMEKYKAPIQADPFSNLDESDLPF"
BASE COUNT          779 a          400 c          319 g          799 t
ORIGIN      
        1 actatatact tcttcccaga acttgtccgt ctccttcaaa ttatctctac ctctcgagtt
       61 cattgggtgg tttgcccatt tattaaacat ggaatcacct ttctcatact catatattta
      121 tgtcctattt caattatatt tatatatgct taaataaatt tgtatcctga aatttattgt
      181 aatatgaagc tgtattttac atatagtaac caaagagtta ataaaataaa aagagcgaac
      241 cattatagat cagctctttt ttcattgctg tttttaatct tatctagaat cacttcaaat
      301 gtttcaaagg tatttacatc taactgacat aaccaaagca agtacttctt ttctttgtaa
      361 gtccaatcaa tattacggta agaggacatt agattatggt accttgctct ttgttcaact
      421 gaagccaatt cccagcgttt ttcaaatatt ttttcttctt ccattattga tccctcgcaa
      481 gacgctacct caaacttaaa taatgggcag cgaaagtcta attccttaga tctaagtata
      541 gcaaacaaat atgaaaaaaa cttagaaggt agtaaaagtt cttccgtctt ttgctttgct
      601 tctattctta atgtgtttcg ttatcggtgc cctacaaaga ttttcattaa ttacacactc
      661 atttattgtt gtgtattttc tgacatttct ctcttgatca attattttaa ttccatgggc
      721 atcctgatag ttcataggag aggagcggct atcagaaata gttgcaccta tctcttgaac
      781 ttcttttaca acttctggaa aatccatcca tcctgcttca gctgtcaaaa gtaataatct
      841 ataatattta tctttccttt tcaatctaag aaatcgtcac cccattcttt ttcttctatt
      901 ataaactatt taacttttag aattcaaaaa ccgtttgtct atttagagtt taccctaaat
      961 agacaaaaat ataacattga aaaatttaga gttctaacgt atgattaggg tgtattataa
     1021 acgttataga aaagtaggga ataaatttga aaattgctta tattagagtt tcatccatcg
     1081 atcaaaatga acaacgtcaa attgaagaaa tgaaaaagtt tggcgctgaa aaaattttta
     1141 tcgaaaaaca atctggagcg acgattagtc atcggccgat tttccaggaa gctttagatt
     1201 ttgtcagaga gcaagatatt tttattgtag aagccattga tcgacttgga cgcaattatg
     1261 acgagattat cgattcagtg aattatctaa agaagaagaa tgtgcgactt attatcacca
     1321 gtttaccgat catggctgaa gcaattggaa atcctctcct agataaattc atcaaagact
     1381 taatcattta aatctcagcc atgattgctg aacaagaacg tactgaatcg aaacgacgcc
     1441 aggcacaagg aattaaaatc gctaaagcaa acggcgtcta taaaggtcgt cctaagttat
     1501 acagtgcaga caccaaagat cctcaacgtc gccttgtcta taggagtatc gttcaggatc
     1561 ttgagaatgg ggttgctatc tcaaagattg caacagacta caatgtgact aggcaaacaa
     1621 tttaccgaat aaaaaaagaa atcgaccaac taattgtatg atttcttttt tgaatcacgt
     1681 aattattaat tagctttgtc atcaatacag acaatcattt tagattcatc tagctaagtt
     1741 gattacattt atgttaaaaa gaatttagtt aacataaata taattgatgc ataaatcgtc
     1801 cttatttaaa ggcatttaac attgacaact tagtataatg tatcttatgt aaactaataa
     1861 gatttgaatt ttattttata cacactcgat aatagatcaa ttttatggtc ccccgcattt
     1921 acgattgcac aagagaaaca taaagtcgac ctattagcat ttcttttcaa ttcaaacatt
     1981 aaaaaagttt atacctctgc tactaaaaaa tgaaattatc tatttaaaat ttaaaacggt
     2041 aaatctgatt catccaaatt ggaaaatgga tcagcctgta ttggagcttt gtatttctcc
     2101 ataataacag atatcttttt ttcatttttt aaaaaatacc ttttaaattt cttatagtcc
     2161 tctgaaatca aaatatagcg tgatggatac tcatcaaaag aagaatcgta ttcttccttt
     2221 aaaattcccg atttacttaa tttttctagt acatcaaata ctctatcact caagtaactc
     2281 cttaaattgt tatcact
//
LOCUS       DACDKM010000196         2259 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20834_2016
            SAMN12640370-rid9891663.denovo.199, whole genome shotgun sequence.
ACCESSION   DACDKM010000196 DACDKM010000000
VERSION     DACDKM010000196.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640370
            Sequence Read Archive: SRR10040891
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2259)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2259)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742835.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742835.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:04:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,012
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 29 of 75
            Pseudo Genes (incomplete)         :: 33 of 75
            Pseudo Genes (internal stop)      :: 19 of 75
            Pseudo Genes (multiple problems)  :: 5 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2259
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640370-rid9891663.denovo.199"
                     /isolate="E20834_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(575..940)
                     /locus_tag="IV334_002775"
     CDS             complement(575..940)
                     /locus_tag="IV334_002775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8944778.1"
                     /translation="MRLWHEKLISKLPRQQLLGQHRECCALRGNGWGKKHATVDYVFH
                     YSPYRLFQYHQLVMDEMEKRGYSPAPEWKNPMYRGKSCEAFFDLPTVERNVPIYPEHD
                     EVYLAECLANLEQKGIYLT"
     gene            complement(1016..1261)
                     /locus_tag="IV334_002776"
     CDS             complement(1016..1261)
                     /locus_tag="IV334_002776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP8944779.1"
                     /translation="MSIEEAILLDTLRCRLNFLRTGRSLSQVAVSSALSIPLFKYRKY
                     EEGEEIPSIIELIYIADFYDISVDYLIGRSETASKLK"
     gene            complement(1331..1585)
                     /locus_tag="IV334_002777"
     CDS             complement(1331..1585)
                     /locus_tag="IV334_002777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8944780.1"
                     /translation="MITLVRVLFWVPAVALVASIVYLMNWNKERFYLAILTLPAIYFM
                     WKVFNYNYFEPDSVFIEELSGLVLSLLIVILYLIRLNKKH"
     gene            complement(1749..2072)
                     /locus_tag="IV334_002778"
     CDS             complement(1749..2072)
                     /locus_tag="IV334_002778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8944781.1"
                     /translation="MLGVIILPFIAILFDLVAAAAYFLQLNYQTSGILFIGMIFQGVI
                     TLLLLVMMLSYKGKRYARIQTKVFVKYVSIRYGIIILSFLVNAIVLFLYVLNYLDINP
                     LIFSR"
BASE COUNT          854 a          371 c          390 g          644 t
ORIGIN      
        1 tttagaaatt attaattttc gtctgaaaac aaaaaaagaa agcgttatta ttttagtcta
       61 ttttcagggg gggaacctta tgataaaaaa actattttta ctatcaatat taacgctagc
      121 aatggcaggt tgttctgact ctggcttagc aactgaactg atcacaaaat caactgaagc
      181 cacgacagag gagttaaaat gtggaaacga tcacagtgac tacagaggct taaatcgacg
      241 atgcatggga atattaggca gaagcaattg taagcatcga tgtagtaatc gaagaaggtg
      301 tagaagaagc aacagaaaaa tcaaaagaaa gatcaaaaga agcaatctgt gctgtgcatc
      361 gtatacagac gtcgaagatg cggtactttt aaaccacgtc aggagatgtt catttatgtc
      421 tagggaagtt ggcgatgaac caaaactaga gtacgctttt agttttgcac gtgaggatga
      481 agataaccct aaaacttacg acattttagc ttgatctaag atggttaaag ctttttaatg
      541 aagtaagagg agatagaagg cttttctagt tatattaggt aagataaatt cctttttgct
      601 ccaaatttgc taaacattct gctagataaa cttcatcgtg ttccggataa atcggcacgt
      661 tgcgctctac agtaggtaaa tcaaaaaatg cttcgcatga tttgccacga tacataggat
      721 ttttccactc tggtgctggg ctgtaaccac gtttttccat ttcatccatg acaagctgat
      781 gatattggaa taatctataa ggagaatagt gaaaaacata atccacagta gcatgtttct
      841 ttccccagcc atttcctctt aatgcacagc attcccgatg ttgtcctaaa agctgttgac
      901 gaggaagctt tgaaatcaat ttttcatgcc ataatcgcat agaaatgtct cctttatata
      961 tagattgtta accttagacg ttaaatgaaa aaaccagcac caaggctgga aaaaatcact
     1021 tcagtttgct tgctgtttct gagcgaccaa ttaggtaatc aacggaaata tcgtaaaagt
     1081 cagcgatata gattaattca atgatgcttg gaatttcttc tccttcttcg tacttacgat
     1141 atttgaataa aggaatactt aacgcgctag aaacagctac ttgagataaa gatcggccag
     1201 ttcttaagaa attcagacga caacgtaaag tatccagtaa aatagcttct tcgatactca
     1261 tttaatcacc tttcattata gtagaatagt tataatgact atagaaagct acacatgcaa
     1321 tcaatagtac tcaatgtttt ttatttaaac gtatcaaata aagaatgacg atcaataaag
     1381 acaaaaccag tcctgataat tcttctataa aaacactatc gggttcaaag tagttgtaat
     1441 taaaaacttt ccacataaag tagatagccg gaagagtcaa aatagctaaa taaaaccgtt
     1501 ctttgttcca attcataaga taaacaatac ttgcgaccag tgcgaccgca ggcacccaaa
     1561 acaaaactct caccaacgta atcatgtaac ggactcctca acgattcata taataaaaga
     1621 taacatgtta ttgcatacat cacaaatgaa atgctaggaa aataaaagaa gaatagatca
     1681 atgaaaaatg aaaaagcaga acagttttct gctgataagc aggaactgtt ccgctgaatg
     1741 tgtcttatct atcgagaaaa aatcaatgga ttgatatcta agtaattcaa tacatataaa
     1801 aagaggacga tcgcattcac taaaaaggaa agaatgatga tcccatagcg aatggaaaca
     1861 tatttaacaa atacttttgt ttgaatacgg gcatatcttt ttcctttata gctgagcatc
     1921 atgacaagaa gcaaaagggt aatcacacct tgaaaaatca ttccaataaa cagaattcct
     1981 gaggtttgat aatttaattg gagaaaataa gctgctgccg caactaaatc gaataaaatc
     2041 gcaataaatg gcaaaataat cactcctaac attgagaata aataaaagaa taacattttt
     2101 tctaaaataa gcaaataaaa attaaagtaa caaaatagtt atgacacaag taaaagaaat
     2161 aagaataaca atattatttt acttatattt tgaaacttgt gagagtttgt cattcgtgct
     2221 atagttgaaa agtaacaaaa ataaatttta tttatgatg
//
LOCUS       DACDKM010000197         2239 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20834_2016
            SAMN12640370-rid9891663.denovo.200, whole genome shotgun sequence.
ACCESSION   DACDKM010000197 DACDKM010000000
VERSION     DACDKM010000197.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640370
            Sequence Read Archive: SRR10040891
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2239)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2239)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742835.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742835.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:04:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,012
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 29 of 75
            Pseudo Genes (incomplete)         :: 33 of 75
            Pseudo Genes (internal stop)      :: 19 of 75
            Pseudo Genes (multiple problems)  :: 5 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2239
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640370-rid9891663.denovo.200"
                     /isolate="E20834_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..377
                     /locus_tag="IV334_002779"
     CDS             <1..377
                     /locus_tag="IV334_002779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HAP8944782.1"
                     /translation="INLFGFPKSVKKQFGKYSNLEINSNDLATYILRYDDKIIEIHLD
                     YFGRVPTRKFQLFTLDECINVDLLNNKISFEENIVKEMPESANDMYEKELKYFFENVV
                     TGKENWNDLYHANEVLKIAEEK"
     gene            378..1130
                     /locus_tag="IV334_002780"
     CDS             378..1130
                     /locus_tag="IV334_002780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylneuraminate cytidylyltransferase family
                     protein"
                     /protein_id="HAP8944783.1"
                     /translation="MEILFTICGRAGSKGFKNKNLKYLAGKPLVHFALAMIDIYQQKH
                     PEHNCTTCLNTDSNELVELTTKLNPSVKIVERKPELATDTVGKIEVIRDSFLQLERED
                     NIFDLIIDLDLTSPLREVSNLEELIDEHLKNLDKDVYFSVVSARRNPYFNMVKRIENE
                     VTIVNKSRYTSRQQTPEVFEMNASMYSFKPEFLKKSNYIFDGQCGIIKMRDYLILDID
                     SEEDFKWLEFLFPKFLEDSSELQDIYNYLYRR"
     gene            1135..2082
                     /locus_tag="IV334_002781"
     CDS             1135..2082
                     /locus_tag="IV334_002781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HAP8944784.1"
                     /translation="MSCYLITGGAGFIGSTLANHLGEEHVVIVVDDLSMGKVENLDMT
                     KNITFIEGDVAEKSLMEEIMKANKFDYIFHLAAVASVADSVARPVETHRVNFESVLMI
                     LELIRKYQPDLKRIVFSSSAAVYGDEPTLPKKEESVIRPLTPYAVDKFAAEQYVLDYC
                     HLYDVPGSAVRFFNVYGPNQNPNSPYSGVISILVDRYKKQIVGEATSFTLFGDGSQSR
                     DFVYIDDVIQALLLVANEEAALGKQFNVGTGKATTLLELIHAINEILGVELTLEYKEE
                     RSGDIHDSLADITKIQSIGYQPKYGILSGMTNYLKTEIN"
BASE COUNT          799 a          290 c          435 g          715 t
ORIGIN      
        1 ttataaacct atttggtttc cctaaatcag taaaaaaaca gtttggtaaa tattcgaatc
       61 ttgaaattaa cagtaatgat ttagcaacat atatattgag atacgatgat aaaattatag
      121 aaattcattt agattatttt ggaagagttc ctacaagaaa atttcaattg tttactttag
      181 atgaatgtat caatgttgac ttattaaata ataaaataag ttttgaagaa aacattgtta
      241 aagaaatgcc tgaatctgct aatgatatgt atgagaagga gttgaaatat ttctttgaaa
      301 acgtagtaac aggcaaagaa aactggaatg atttatatca tgcaaacgaa gtattgaaaa
      361 tagctgagga gaaataaatg gaaattttat ttactatttg tggtagagct gggtctaagg
      421 ggtttaaaaa taaaaatttg aaatatttgg caggaaagcc cctagtccat ttcgctttag
      481 caatgataga tatctatcaa caaaaacatc ctgaacataa ttgcacgact tgtttgaata
      541 cggacagtaa tgagttagta gaattgacga ctaagttaaa tccgtctgtg aaaattgtag
      601 aacgtaaacc tgaattagct acagatactg tagggaaaat tgaagtgata cgtgattcat
      661 tcttacaact agaaagagaa gataatatat ttgatttaat tatagattta gatttgactt
      721 cacctttacg tgaagtttct aatcttgaag aattaataga tgaacatcta aaaaatttag
      781 ataaagacgt ctacttttca gttgtttctg caagacgtaa tccctatttt aatatggtaa
      841 aaagaattga aaatgaagtt actatagtta ataaatcaag atacacatct aggcaacaga
      901 cacctgaagt ttttgaaatg aatgcctcaa tgtattcttt taaacctgaa tttctaaaaa
      961 aaagtaatta tatttttgat gggcaatgtg ggataataaa aatgagagat tatctaattt
     1021 tagatattga tagtgaagaa gattttaaat ggttagaatt tttgtttcct aaatttttag
     1081 aagattcttc ggagcttcaa gatatctata attatttata tcggaggtaa aaggatgagc
     1141 tgttatctta tcaccggtgg ggctggcttc attggttcta ccttagccaa tcatttagga
     1201 gaagaacatg tggtaatcgt cgtcgatgac ttatcaatgg gaaaagtaga aaatcttgat
     1261 atgacgaaaa atatcacttt tattgaggga gatgtcgctg agaaatcctt gatggaagag
     1321 atcatgaaag ctaataaatt tgattatatc tttcatttag cagcagttgc tagtgtggca
     1381 gattcagttg cccgaccagt agaaacacat cgtgtgaatt ttgaaagtgt attaatgatt
     1441 ttagaattga tccgcaagta tcaacctgat ttaaaacgga tcgtgtttag ttcttcagcg
     1501 gctgtttatg gggatgaacc gactttacca aaaaaagaag agtccgtgat tcgcccattg
     1561 acgccttatg cggttgataa attcgctgca gaacaatatg tgctagatta ttgtcattta
     1621 tatgatgtac ccggtagcgc agttcgtttt ttcaatgtct atggtccaaa tcaaaatccg
     1681 aattctccat acagtggtgt gatttctatt ctcgtagatc gctataagaa acaaatcgta
     1741 ggagaagcaa cttcatttac attatttggt gatggaagcc agtctagaga ctttgtttat
     1801 atagatgatg taatccaagc attactttta gttgcgaatg aggaagctgc actggggaaa
     1861 caattcaatg ttggaacggg taaagcaacc acacttttgg aattgattca tgcaatcaat
     1921 gaaattctcg gtgtagaatt gacacttgaa tacaaagaag agcgttcggg tgatatccat
     1981 gactctctag cagatattac taagatccag tcaattggct atcaaccgaa atatggtatt
     2041 ttaagtggaa tgactaatta tttgaaaact gaaatcaatt aaaacatact aagataagat
     2101 tagagggtaa attcaggctc tttgtcaata aggactgatg agttttgtaa aatcaaatct
     2161 gggatagaag ggctctttgt caaatgatgt tggtaaagag aaatcttaga ataatttgga
     2221 cagaacagaa agaagaaaa
//
LOCUS       DACDKM010000198         2232 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20834_2016
            SAMN12640370-rid9891663.denovo.201, whole genome shotgun sequence.
ACCESSION   DACDKM010000198 DACDKM010000000
VERSION     DACDKM010000198.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640370
            Sequence Read Archive: SRR10040891
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2232)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2232)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742835.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742835.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:04:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,012
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 29 of 75
            Pseudo Genes (incomplete)         :: 33 of 75
            Pseudo Genes (internal stop)      :: 19 of 75
            Pseudo Genes (multiple problems)  :: 5 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2232
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640370-rid9891663.denovo.201"
                     /isolate="E20834_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..544
                     /locus_tag="IV334_002782"
     CDS             <1..544
                     /locus_tag="IV334_002782"
                     /inference="COORDINATES: protein motif:HMM:NF033516.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HAP8944785.1"
                     /translation="ISKENLLNQDFSTETICEKWAADITYIPTKKNGWCYLSSIMDLH
                     TKRIISYTFSKRMTVDCVIQTLNKAKIHYHIPEGMILHTDLGSQYTAREVEQWLKTNK
                     IRHSYSRKGTPYDNAGIESFHASLKKEEVYTTSYSDFEEANRALFSYIEGFYNRNRIH
                     SSIHYLTPQEFEELAKEKMA"
     gene            complement(900..1472)
                     /locus_tag="IV334_002783"
     CDS             complement(900..1472)
                     /locus_tag="IV334_002783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000170424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAP8944786.1"
                     /translation="MTKYGYARVSTASQSTKEQIQQLLENCVEKKNIFSEKFTGTKRD
                     RPKFNQLLAGLKPGDEIVVTKLDRFARNTREALEIIEPLLDDNVTIKVLNLGTIENTS
                     MGRMVTRTLLSVAEMERDMIVERTQEGKMFARKNNPNFREGRPKATITPKKRHAYELL
                     TSGKSYKEVEAITGFSRSTLFRIKKKIEES"
     gene            complement(1488..2093)
                     /locus_tag="IV334_002784"
     CDS             complement(1488..2093)
                     /locus_tag="IV334_002784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell filamentation protein"
                     /protein_id="HAP8944787.1"
                     /translation="MILENKLGLTNQVELAKVEEKLSKQKAKELYDCGKINEIEVGTF
                     KGLSEIHEFLFSDIYDFAGKIRSVNIAKGNFRFAPVMYLEHSLQHIDQMPQTSFEEII
                     KKYVEMNIAHPFREGNGRSTRIWLDLILKEELQKVVDWNLIDKADYLSAMERSPINDL
                     EIRYLISNALTDKIDDRELYMKGIDVSYFYEGYSEYTIDDL"
BASE COUNT          700 a          454 c          338 g          740 t
ORIGIN      
        1 catttcaaaa gagaatctct taaatcagga cttttctact gaaacgatct gtgagaaatg
       61 ggcagctgac attacgtaca ttcctactaa gaaaaatggt tggtgttact tatcttcaat
      121 catggattta cacacgaaaa gaattattag ttatacattt tcaaaacgaa tgactgtgga
      181 ttgtgtcatt caaacattga ataaagcaaa aatacattat cacattccag aaggaatgat
      241 tctacacact gacttgggca gccaatacac agcaagagaa gtggaacaat ggcttaaaac
      301 caacaaaata aggcattctt atagtagaaa aggaacacct tatgataatg ctggaatcga
      361 atctttccat gcctcattga aaaaagaaga agtctacacg actagctact cagattttga
      421 agaagcaaat cgagcgctat ttagctacat tgaaggattt tataaccgaa atcgaatcca
      481 tagctcgatt cactatctaa ccccacagga atttgaagaa ttagcaaaag aaaaaatggc
      541 ttaaccgtct ctactaaaga caatcaacac cgtagggata tggagataaa atcaatagag
      601 tcaatagatt atttatcgat aataacacta aaaaagccac gttcctaaac gtgacttacg
      661 ctgaacgata tgtgttgttt ggtagacgaa caaatcgttc attataaata tatgatggta
      721 tcctaatata gtaagcgtgg ctagcatacc tttaattact ttacctttta cagttaccat
      781 acacattttt tgagcagaaa tcaagtaaat aaaaaaatat tatatttcac aacatgctaa
      841 tagactcata tctagaacgc attaagtatt tacctgtctg gttcgctgag acttcttttt
      901 taactttctt caattttctt ctttattcta aaaagcgtac tgcgactgaa accggttata
      961 gcttcaactt ccttatacga tttacctgaa gtaagcagtt catatgcgtg cctcttcttc
     1021 ggtgtgattg ttgctttcgg acgtccttct ctgaaattag gattattctt tcttgcaaac
     1081 atttttcctt cttgggtacg ttccacaatc atatcacgtt ccatttctgc tacactcaac
     1141 aacgtccgtg tcaccattcg ccccatggac gtattttcaa tcgttcctag attcaaaacc
     1201 ttgatcgtta cgttatcatc gagtaaaggt tcaatgatct ctaatgcttc tctcgtgtta
     1261 cgagcaaatc gatcaagctt cgtaacaact atttcatctc caggcttcag accagccaac
     1321 agctgattga attttggccg atcccgtttt gtacccgtaa atttttctga aaaaatattc
     1381 ttcttctcta cgcagttctc aagcaactgt tgaatttgtt cttttgtact ttgagaagcg
     1441 gttgagacac gtgcatatcc atatttcgtc atttggtcca cctactttta caaatcatct
     1501 atcgtatact cagaataccc ttcatagaaa tagctgacat cgattccttt catatataac
     1561 tcgcgatcgt caattttatc agtcagagca ttggaaatta gatagcgtat ttccaaatcg
     1621 tttatgggac ttcgctccat tgcagaaaga tagtctgctt tatcgattaa gttccaatct
     1681 acaacttttt gcaattcttc ctttaaaatg agatccagcc aaattctagt acttcggcca
     1741 tttccttctc gaaatgggtg agcgatattc atctccacat atttcttgat aatctcttca
     1801 aaagaagttt gtggcatctg atcaatatgc tgtaacgaat gttctaaata cataactggt
     1861 gcaaatcgaa aatttccttt tgcaatattg accgatcgga tttttccggc aaaatcgtaa
     1921 atatccgaaa atagaaattc atgaatttca gataaacctt taaatgtacc aacttcgatt
     1981 tcatttattt ttccacaatc gtacaattct ttcgcttttt gtttactgag tttttcttca
     2041 accttagcca actcaacttg attggttaat cccaatttat tctctaaaat catgcgaaat
     2101 ctccttaact ttatgacata agtttatgaa acactctcaa ccccattata tcgcgatttt
     2161 gtttatgtgt caaaaactat taagtttctg acccccgata gatttttcaa gtgtctctat
     2221 tttgcacagt ct
//
LOCUS       DACDKM010000199         2199 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20834_2016
            SAMN12640370-rid9891663.denovo.202, whole genome shotgun sequence.
ACCESSION   DACDKM010000199 DACDKM010000000
VERSION     DACDKM010000199.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640370
            Sequence Read Archive: SRR10040891
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2199)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2199)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742835.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742835.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:04:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,012
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 29 of 75
            Pseudo Genes (incomplete)         :: 33 of 75
            Pseudo Genes (internal stop)      :: 19 of 75
            Pseudo Genes (multiple problems)  :: 5 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2199
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640370-rid9891663.denovo.202"
                     /isolate="E20834_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            108..2192
                     /gene="cadA"
                     /locus_tag="IV334_002785"
     CDS             108..2192
                     /gene="cadA"
                     /locus_tag="IV334_002785"
                     /EC_number="7.2.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cadmium-translocating P-type ATPase"
                     /protein_id="HAP8944788.1"
                     /translation="MKSYRLEGLDCANCAMKIEKGVQKINGVKEATVNFTSGKLTIDA
                     EEDHLATIEQETKKVVKELEPDVKVTEIDKEKVSEHGNEKERNTLFRILFSLAGIALL
                     LLFDFNEPIRLIGYLLIYLLIGYDVVKKAVMNIVKGKIFDENFLMSVATIGAMLIGEY
                     PEAVAVMLFYQIGEYFQGLAVSHSRKSIRELMAIRPETAHVQTTEGLMTVNPEDVQIG
                     QFVLVKPGERVPLDGTIIEGESLVDTSALTGESVPKSVYVGETVLSGFINKNKPFLVQ
                     VEKSYENSTISKLLELVENASSKKAPAENFITKFARYYTPVVVGLAVLLAVLPPLVVS
                     GAAFSEWIYRALTFLVISCPCALVISVPLSFFGGIGGASKIGVLVKGSNYLELLAQTE
                     TVVFDKTGTLTKGDFSIQTIDTKIDPKKFMQYIASAEQFSTHPIAQSVLEGYAGPLLP
                     TTNIQEFAGEGILAEVDGKQVLAGNHKLMERFEISFPLSQEIGTLLYLAIDQSYSGYL
                     VIADTLKEDAVDALVQLKQAGVKNTVMLTGDSKKIADHIGKQVGVDKIYSELLPEDKV
                     QRLEEILQRNNKKTAFVGDGINDAPVLARADVGIAMGGLGSDAAIEAADVVIMNDQPS
                     KIAEAIHLAKKTLKIVKQNIVFAIGIKILVLALGAFGFASMGDAVFADVGVTVLAVLN
                     AMRSLHVKKEDE"
BASE COUNT          720 a          349 c          506 g          624 t
ORIGIN      
        1 cagatgtttt tcattttttc tttcggatac cttgacatat gaataaccat tcatttataa
       61 ttagttatga agagatgttc atatgttgag gcgaggaggt cattaaaatg aaaagttaca
      121 gattagaagg tttagattgt gcgaactgtg caatgaaaat cgaaaaagga gttcagaaga
      181 tcaatggagt caaggaagca acggtaaact tcacttctgg aaaactaacg attgacgcag
      241 aggaagatca tttggctacg attgaacaag aaacaaaaaa agtagtgaag gaattagaac
      301 cagatgtcaa agtaacagaa atagataagg aaaaagtatc tgaacatgga aatgaaaaag
      361 agagaaatac gctgttccgg attttatttt cccttgcagg tatcgcttta ttgctattat
      421 ttgattttaa tgaaccaatt cgcttgatag ggtatttatt gatttatctg ctgataggat
      481 atgatgtagt gaagaaggca gtgatgaata tcgtcaaagg aaaaatcttt gatgagaatt
      541 ttcttatgtc tgttgctacg attggcgcta tgttgatcgg tgaatatcca gaagccgtag
      601 cagtgatgct attttatcaa atcggcgagt atttccaagg attggcagtc agtcattcaa
      661 gaaaatcgat tcgcgaattg atggcgatcc gtccagaaac agctcacgtg cagacgacgg
      721 aaggattgat gaccgtgaac cctgaagacg tgcagatcgg tcagtttgta cttgtgaagc
      781 ctggtgaacg tgtaccgtta gatggaacga tcatcgaagg agaatcatta gtcgatacgt
      841 cagcacttac aggagaatca gtccctaaat ctgtatatgt tggtgaaaca gtattaagcg
      901 gatttatcaa taaaaataaa ccatttttag ttcaagtaga aaaatcttat gaaaattcta
      961 ctatcagcaa attattagaa ttagtagaaa atgccagcag taaaaaagca ccagctgaaa
     1021 atttcattac taaattcgcg cgctattaca caccagtcgt tgtaggattg gccgttttat
     1081 tagctgtttt acctccactt gttgtttctg gagcggcgtt ttctgaatgg atctatcgag
     1141 cattgacatt tttagtgatc tcatgtccat gtgcattagt gatttccgtg ccacttagtt
     1201 tctttggcgg tatcggaggc gcaagtaaaa tcggtgtatt agtaaaaggt tcaaattact
     1261 tggagttgct tgcgcaaacc gagacagtgg tattcgataa aaccggcaca ttaacgaaag
     1321 gtgacttttc gatccagacg atcgatacaa aaatcgatcc aaagaaattc atgcaatata
     1381 ttgcgagtgc agaacagttt tctacacatc cgattgctca atctgtttta gaaggctacg
     1441 caggtccgct tttaccaaca actaatatcc aggaatttgc aggtgaaggg atcttagctg
     1501 aagttgatgg caaacaagta ttagcaggta atcacaaatt gatggaaaga tttgaaatct
     1561 cttttccttt aagtcaagaa atcggtacat tgctttatct agcgatcgat caaagttaca
     1621 gtggttatct tgttattgca gataccttga aagaagatgc cgtagacgca ttggttcaat
     1681 taaaacaggc tggtgtgaag aacacagtta tgttgacggg cgactcgaaa aaaattgctg
     1741 atcatatcgg gaagcaagta ggcgtggaca agatatatag cgaattgctt cctgaagata
     1801 aagtgcagcg gttagaggaa atcttacaaa gaaacaataa aaagaccgca tttgtcggag
     1861 atggaatcaa cgatgcaccg gtgctagcaa gagcagatgt aggaatcgca atgggtggat
     1921 tgggaagcga tgctgcaatt gaagcagcag atgtagtcat catgaatgat caaccatcga
     1981 agattgcaga agcaatccat ttagcaaaga aaacattgaa aatcgtcaaa caaaacattg
     2041 tctttgctat tgggataaaa attttggtac ttgccttagg tgcttttggt tttgcatcaa
     2101 tgggagatgc agtatttgca gatgttgggg tgacagtatt agctgttttg aatgccatgc
     2161 gaagcttgca tgtaaaaaaa gaggacgaat gaacgtcct
//
LOCUS       DACDKM010000200         2126 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20834_2016
            SAMN12640370-rid9891663.denovo.204, whole genome shotgun sequence.
ACCESSION   DACDKM010000200 DACDKM010000000
VERSION     DACDKM010000200.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640370
            Sequence Read Archive: SRR10040891
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2126)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2126)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742835.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742835.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:04:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,012
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 29 of 75
            Pseudo Genes (incomplete)         :: 33 of 75
            Pseudo Genes (internal stop)      :: 19 of 75
            Pseudo Genes (multiple problems)  :: 5 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2126
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640370-rid9891663.denovo.204"
                     /isolate="E20834_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(85..339)
                     /locus_tag="IV334_002786"
     CDS             complement(85..339)
                     /locus_tag="IV334_002786"
                     /inference="COORDINATES: protein motif:HMM:NF024673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HAP8944789.1"
                     /translation="MTATEIRNNKLKKKISTIFFTNKQRYGATKIHQVLLKEGISVSL
                     KHVQKLMKQLNLRSIVVKKYRPQRSNKPIMDRLQLTRQKN"
     gene            complement(450..704)
                     /locus_tag="IV334_002787"
     CDS             complement(450..704)
                     /locus_tag="IV334_002787"
                     /inference="COORDINATES: protein motif:HMM:NF013677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAP8944790.1"
                     /translation="MTRYEENFKQMIVELNQTGRSVRGLAKEYGLSEATIYKWKNLYL
                     PDQSTGLTGKEVAELRKENARLNEELEILKKAAAIFSRKT"
     gene            complement(897..1172)
                     /locus_tag="IV334_002788"
     CDS             complement(897..1172)
                     /locus_tag="IV334_002788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001196543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein PrgO"
                     /protein_id="HAP8944791.1"
                     /translation="MALLPTNKDKKKIGAEDISIKDDINVELGERKYTAKERKPVQVD
                     PPVLKTIRDLSYAKDIPMYEIVKLAMDAYVDTLNDEERIIYNRRSKD"
     gene            complement(1144..2097)
                     /locus_tag="IV334_002789"
     CDS             complement(1144..2097)
                     /locus_tag="IV334_002789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HAP8944792.1"
                     /translation="MENLRTAFKTSDKLGKVVMLTGRKGGIGKTTDNDLLAIVSSQLF
                     EKDVLLIDYDQQRNTTSNIGSTYQITSFDRSMSAAIKKGDWVSGITQVSPHLYIMAGS
                     PGSEELNEYLSEKYPDRRKRSLAFIKPLEELRKNFDYIFIDCPPSTDNVVRAFLTAAD
                     YIIAMQELKRYAMEGTEDFINKVLVPIVTNFEESHLQIIGILPVLFSVRRSSQHANYQ
                     KTIDKYGESNIFKSIVKGSDRLEMYGENGIQLNDYVDRRWWAIFADIFTELEERIEYY
                     EQHGDIEGFDYTPKYADSMANHILPLGKEIKLNGIITNEQR"
BASE COUNT          635 a          392 c          310 g          789 t
ORIGIN      
        1 tgcttgaatt cttgtgaaaa agttcttcgt gttctcttag acataaaaat tcctcctggt
       61 atgttttctt ctagtctaca caccttaatt tttctgtcta gttaattgta gcctatccat
      121 gatcggttta ttagaccttt gaggtctata ttttttaact acaatcgacc ttaaatttaa
      181 ttgtttcatt agcttttgga catgtttaag agatactgaa atcccttctt ttaataaaac
      241 ttgatggatc tttgtggcac catagcgttg tttattcgta aaaaaaattg ttgaaatctt
      301 ctttttcaac ttattatttc tgatttctgt agctgtcaac ggtttattct tataaaaata
      361 atagacactt ctagggattt ctaataaccg acacaacaaa gaaacagtgt aatccttaca
      421 ccatttttca ataaattgaa caagcgaatt taggttttcc gagagaatat ggctgcggct
      481 ttttttaaga tttcaagttc ctcatttaaa cgagcatttt cttttctcag ttcagctact
      541 tcttttccag tcagtcctgt ggactgatca ggtaaatata aattcttcca tttgtaaatc
      601 gttgcttcag ataagccata ttctttcgct aaccctcgaa cagaacgtcc agtttgattc
      661 agttcaacaa tcatttgttt aaaattttct tcatatcggg tcataaaaat tcatcctctc
      721 gtagataagt ttaatttatt cttgtctcta cgaaaagctg tctaagattt gatactaaca
      781 ccaaatgttg tataccccca aacaatacac atattttgaa ggattaggat taataaagct
      841 aaaaatatag taagtatact aaatatattt actatatcta gtatacttac tatattttaa
      901 tcttttgatc gtctattata tataatccgt tcctcgtcgt ttaaagtatc aacataggcg
      961 tccattgcaa gtttgactat ttcatacata ggaatatctt ttgcataact aagatcccga
     1021 atggttttta gtacgggagg atctacttga actggttttc tttctttcgc agtgtacttt
     1081 cgttccccta actcgacatt aatatcatct ttaattgaaa tatcttcagc accaattttc
     1141 ttcttatctt tgttcgttgg taataatgcc atttagttta atctcctttc caagaggtag
     1201 gatatgattg gccattgagt cagcatattt aggtgtataa tcaaatcctt caatatcacc
     1261 atgttgttca tagtattcaa ttctttcttc taattctgta aagatgtctg caaaaatagc
     1321 ccaccatctt cgatctacat aatcatttaa ttggatacca ttctcaccat acatttctaa
     1381 ccgatccgag cctttaacaa tgcttttaaa aatgtttgat tcaccatact tatcaattgt
     1441 tttttgatag tttgcgtgtt gagaggaacg tcgcactgag aaaagaacag gcaaaattcc
     1501 aatgatttga agatgagact cttcaaaatt cgtgacaatc ggaactaaaa ctttattaat
     1561 aaaatcttct gttccttcca ttgcgtaccg ttttaattct tgcattgcaa taatataatc
     1621 tgcagcagtt aagaatgcac gaaccacatt atctgttgat ggtgggcagt cgataaagat
     1681 ataatcgaaa ttttttctca attcttctaa aggtttaatg aaggccaatg atctcttacg
     1741 acgatcagga tatttttcgg acaagtattc attaagttct tcagatccag gagagccagc
     1801 cataatatat aaatgtggac ttacttgagt aatacctgaa acccagtccc cttttttgat
     1861 tgctgcagac atggatcgat caaagctagt aatttgatac gttgatccaa tgttggaagt
     1921 agtatttctt tgttggtcat aatcaattag caacacgtct ttttcaaaaa gttgactaga
     1981 aacaatcgct aataaatcat tgtctgtagt cttaccaatg ccgccttttc tacctgtaag
     2041 cattacaact tttcctagtt tatctgatgt tttaaatgca gtccgtaagt tttccatagt
     2101 tccctccaat ttcctatact aaatat
//
LOCUS       DACDKM010000201         2104 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20834_2016
            SAMN12640370-rid9891663.denovo.205, whole genome shotgun sequence.
ACCESSION   DACDKM010000201 DACDKM010000000
VERSION     DACDKM010000201.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640370
            Sequence Read Archive: SRR10040891
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2104)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2104)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742835.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742835.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:04:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,012
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 29 of 75
            Pseudo Genes (incomplete)         :: 33 of 75
            Pseudo Genes (internal stop)      :: 19 of 75
            Pseudo Genes (multiple problems)  :: 5 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2104
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640370-rid9891663.denovo.205"
                     /isolate="E20834_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            169..1539
                     /locus_tag="IV334_002790"
     CDS             169..1539
                     /locus_tag="IV334_002790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4357 domain-containing protein"
                     /protein_id="HAP8944793.1"
                     /translation="MMDKSNEEQTTLYTKYWRRLEEVFDNSKGMEDFFRYYLAAITGE
                     YSAKHILYQAFKDYWHKERDVSSDAELLQKLVRYAGYFARLYYNKPEGKYSEVLSDFQ
                     SMESMMPAPFVLGLSEWYYHDQFITEDQYIDAIKVVNDYQLRRYFNGDDTSRVSKAFP
                     SYLKSVRKYAKANGYENIVDIVIYVLVTRNQSNNMALPSDKALKSNLLNANAYAMRLA
                     RWLLEKIENRENSATLDMSNLSIEHIMPQTSTSYWEEKAGTSGEEYTGLVNTIGNLTL
                     VTNPDNSAAGNKDFETKKKIFEDTLHIRMNKDLYELTEWTSSDISARSEALINELITM
                     YPYLRSSGDYEHDGNREIFLEAQGIKATGYLNEDETVIIHSGSEIYSKIKDIASDSLD
                     ETRQELLDNGIIEETIGGLQFVQDYTASSVSNAAALLLGGSRNGWDYWKYDNGISIND
                     SLRNKK"
     gene            1560..1844
                     /locus_tag="IV334_002791"
     CDS             1560..1844
                     /locus_tag="IV334_002791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8944794.1"
                     /translation="MDKNKLRYAILKLRDEKKNPFIELKGQVPEEDILEQTKFLSRNG
                     LLESMVWADNTVHIWGSVSLEGEKYLVENSGLAKAYALAKEVKNWIPFWG"
BASE COUNT          731 a          308 c          444 g          621 t
ORIGIN      
        1 ttgatttacc aacatgttta gacattttcc tctaggaaaa aattaactag caccacgcgt
       61 attgtttata ttaaattaga tgaccgtgat aatgcacagc aaatttttga aagtatcaat
      121 tctaccggtg aaagattgac tgcctcggat ttgattcgaa acttcatcat gatggataaa
      181 tctaatgaag agcagactac gctttatact aaatattggc gtaggttgga agaagtcttt
      241 gataattcta aaggaatgga agactttttc agatactacc ttgctgcaat aactggggag
      301 tattcagcaa agcatattct ttatcaagca ttcaaagact attggcataa agagcgcgat
      361 gtatcaagtg atgccgaatt gctccaaaaa ttagtgcgat atgctggtta ttttgcgaga
      421 ctttactata acaaacctga aggaaaatac tcagaagtat tgagcgattt ccaaagtatg
      481 gaaagtatga tgcctgcacc ttttgttttg ggattatctg aatggtacta tcatgaccaa
      541 tttatcactg aagaccaata tattgatgcg attaaggtag tgaatgatta tcagcttagg
      601 agatatttta atggggatga tacaagcaga gtgtcaaaag catttccttc atatttgaaa
      661 agtgttagga agtacgctaa agctaatgga tatgaaaata tcgttgatat tgtaatttat
      721 gttcttgtta ctagaaatca atcaaataat atggcacttc catctgacaa ggcattgaaa
      781 tctaatctat tgaatgccaa tgcttatgcg atgagattgg cccgttggtt acttgaaaaa
      841 atagagaatc gagaaaattc agcaacactt gatatgagta acttgtccat tgaacacatc
      901 atgccacaga ctagtacttc atattgggaa gagaaggctg gtacatcggg ggaagaatac
      961 acaggattag taaataccat tggtaattta acactcgtta caaatcctga taattcggcc
     1021 gcgggaaata aagactttga gaccaaaaag aaaatctttg aagataccct tcatattcgt
     1081 atgaataaag atttgtacga attgactgag tggacatcat cggatatttc tgccagaagt
     1141 gaagctctaa tcaatgaact gattactatg tatccctatt tgagatcgtc gggcgactat
     1201 gaacacgatg ggaataggga aattttctta gaggcgcaag gaataaaagc aactgggtat
     1261 ttgaacgaag acgagaccgt gattatacat tcaggttcag aaatttattc taagattaaa
     1321 gatattgcat ctgattcgct agatgaaact cgccaagagc tgttggataa tggaatcatt
     1381 gaagaaacaa ttgggggact acaatttgtt caagattaca ctgcctcatc tgtttcgaat
     1441 gcagcagctc tgctattagg gggtagccgg aatggttggg actactggaa atatgataac
     1501 ggaataagta ttaatgattc gctaagaaat aagaaataga gcatagaggt ggtgttaggt
     1561 tggataagaa caaactaaga tatgcaattc ttaaattgcg tgatgaaaaa aagaatcctt
     1621 ttattgagct taaagggcaa gttccagaag aagatatttt agaacaaact aaatttcttt
     1681 cgcgcaatgg cttactagaa tccatggttt gggcggataa cactgttcat atctggggga
     1741 gtgtttctct ggaaggggaa aaatatttag ttgaaaactc aggtcttgct aaggcgtatg
     1801 cattggcaaa agaagttaaa aattggattc cattttgggg ctaagataag tttttaaacg
     1861 gagaaggtag caaatcataa ttggtttgtt gccttttttg attgcccaaa aagaaaaatg
     1921 acaaagtata gagataaatg acatagtaca cgggaaaaat gacattgtat caccgacaac
     1981 gtctaaatag atgttgatat atatacgttc cgtacaaata attataaaaa tttaaatgat
     2041 ttgagtagaa gtatcagtga atatctcaag gggaatagct atctcaagat tcgattttca
     2101 cata
//
LOCUS       DACDKM010000202         2078 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20834_2016
            SAMN12640370-rid9891663.denovo.206, whole genome shotgun sequence.
ACCESSION   DACDKM010000202 DACDKM010000000
VERSION     DACDKM010000202.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640370
            Sequence Read Archive: SRR10040891
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2078)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2078)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742835.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742835.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:04:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,012
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 29 of 75
            Pseudo Genes (incomplete)         :: 33 of 75
            Pseudo Genes (internal stop)      :: 19 of 75
            Pseudo Genes (multiple problems)  :: 5 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2078
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640370-rid9891663.denovo.206"
                     /isolate="E20834_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..654
                     /locus_tag="IV334_002792"
     CDS             <1..654
                     /locus_tag="IV334_002792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HAP8944795.1"
                     /translation="LKYTSDPYIIIGAGGHAKVIIETLRLENKLIYGFFDDKQNKMQD
                     LKYLGKINELNSWADSKKKIYKFIVAIGDCVVRKKIIKKLNMPSEQYGIAVHPSAIVA
                     KEISIGYGTVIFANAVINPDAVIGEHAIINTGSIVEHDCRINNYVHLSPGVCLAGGVH
                     VGVGTQIGIGSQCIQMKKIGSWCMIGAGSTIVKDIPSHSLAYGNPAKIKKEGINFEF"
     gene            644..1786
                     /locus_tag="IV334_002793"
     CDS             644..1786
                     /locus_tag="IV334_002793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HAP8944796.1"
                     /translation="MNSRILLSSPHMGGTEEKYVAKAFKDNWIAPLGENVDKFEESIT
                     KYTGSKGGASALSSGTAAIHLALRLLNVGKNDIVFAPSFTFIATVNPILYQGAVPVFI
                     DSEPNTWNMSPVALESALEEAERNNQLPKAIIVVNLYGQMADYERILAIANQFNVPVI
                     EDAAESLGSTYKGISSGLFGTIGIYSFNGNKIITTSGGGALVSSDKKYVEHSRFLATQ
                     ARDQAPYYQHSEIGYNYRLSNISAGIGRGQMEVLNDRVAKRREIFNRYKEAFSGIDRI
                     EMMPEVDGMVPNRWLSTLTINDYNSIDQVVNVINKLNEKNIETRALWKPLHLQPVFQG
                     VKMYYHDNNLSVSEKLFNSGLCLPSGSNMTDSEQEFVIDEFKKIFK"
     gene            1799..>2078
                     /locus_tag="IV334_002794"
     CDS             1799..>2078
                     /locus_tag="IV334_002794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HAP8944797.1"
                     /translation="MNIGIIGLGSMGKRRLRLIQEHFNDILLCGVDTNEARRKEVEKE
                     FGIPTYNSLTEAKNLFEINSVFVCTSPITHEFIIKEALNLDCNIFTEIN"
BASE COUNT          743 a          271 c          425 g          639 t
ORIGIN      
        1 ttaaaatata ctagtgatcc atacattatt attggggctg gcggacacgc aaaagtcatt
       61 attgaaacct taagattaga aaataagtta atatatggat tttttgatga taagcaaaat
      121 aaaatgcaag atctgaaata tcttgggaaa ataaacgaat tgaatagctg ggcggatagc
      181 aaaaaaaaaa tctataaatt tattgtagct attggtgact gtgtagtaag aaaaaagatt
      241 ataaaaaaat tgaatatgcc aagtgagcaa tatggaatag ctgttcatcc aagtgctata
      301 gtggcaaaag aaatttcaat tggttatgga actgttattt ttgcgaatgc tgtaattaac
      361 ccagatgctg taattggaga gcatgctata ataaacacag gtagcattgt tgagcatgat
      421 tgtagaatta ataactatgt tcatttatct ccaggtgtat gcttagcggg tggagtacat
      481 gtaggtgtag gaactcaaat aggaattggt tctcaatgta tccagatgaa gaaaattgga
      541 tcttggtgta tgataggcgc agggagcact attgttaagg atattccctc acattcctta
      601 gcttatggca atccagctaa aattaagaaa gaaggaatta attttgaatt ctagaatttt
      661 attatcaagt cctcatatgg gaggaacaga agaaaaatat gttgctaagg cttttaaaga
      721 taattggata gcgccattag gtgaaaatgt ggataaattt gaagaaagta ttacaaaata
      781 tactggttct aagggcggag cttctgcgtt aagttcaggt acagctgcca tacatcttgc
      841 cttaagactg cttaatgttg gaaaaaacga tattgtcttt gctccttcat ttacttttat
      901 agctacagta aatcctattt tatatcaagg ggctgtacca gtgttcattg actcagaacc
      961 taatacatgg aatatgagcc ctgttgcact agagagcgca ttagaagaag ctgaaagaaa
     1021 caaccaatta cctaaggcaa tcatcgtagt gaatttatat ggtcaaatgg ctgattatga
     1081 acgaatttta gctattgcaa accaatttaa tgttccagtg atagaagatg cagcggaatc
     1141 gttaggttct acttataagg gaatttcgtc aggcttattt gggacaattg ggatctattc
     1201 atttaacggt aataagatca tcacaacctc aggtggagga gctctagtgt ctagtgataa
     1261 aaaatatgtt gagcattcaa gatttttagc aacacaagca agagatcaag caccgtatta
     1321 tcaacatagc gaaataggtt ataattaccg tctaagcaat atttctgccg gtatcggacg
     1381 tggacaaatg gaagtattga atgatagagt agctaaacga agagaaattt tcaatcgata
     1441 taaggaagct ttttctggaa ttgatagaat tgaaatgatg ccagaagtgg atggcatggt
     1501 gccaaatcgt tggttgtcta ctttaacgat caatgattat aattctattg atcaagtagt
     1561 taacgtaata aataaactaa atgaaaaaaa cattgaaaca agagctttgt ggaaaccact
     1621 acatttgcaa ccagtatttc aaggggtaaa aatgtattac catgataata atcttagtgt
     1681 gtcagaaaag ttatttaata gcggcttatg cttaccttct ggaagtaata tgacagatag
     1741 tgaacaagaa tttgttattg atgagtttaa aaaaatattc aagtaggtgt gatattttat
     1801 gaacatagga attattggtt taggttccat ggggaaaaga agacttagac tcattcaaga
     1861 acactttaat gatattttac tatgtggtgt tgatactaat gaggcgagac gaaaggaagt
     1921 tgaaaaggaa tttggaatac caacttataa tagtctaaca gaggcaaaaa atttgtttga
     1981 aataaattca gtttttgttt gtacatcccc tataacccat gaatttatta taaaagaggc
     2041 tttaaattta gattgtaata tctttacgga aataaatt
//
LOCUS       DACDKM010000203         2003 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20834_2016
            SAMN12640370-rid9891663.denovo.207, whole genome shotgun sequence.
ACCESSION   DACDKM010000203 DACDKM010000000
VERSION     DACDKM010000203.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640370
            Sequence Read Archive: SRR10040891
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2003)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2003)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742835.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742835.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:04:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,012
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 29 of 75
            Pseudo Genes (incomplete)         :: 33 of 75
            Pseudo Genes (internal stop)      :: 19 of 75
            Pseudo Genes (multiple problems)  :: 5 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2003
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640370-rid9891663.denovo.207"
                     /isolate="E20834_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..378
                     /locus_tag="IV334_002795"
     CDS             <1..378
                     /locus_tag="IV334_002795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HAP8944798.1"
                     /translation="KNVSTEDFFNYCRLEESGVTVTSGTDLPLFITDIPESIIRAVFR
                     RFPEGEEVWEKETSFTTTELLKSFTSNSFKVNGLSEYGEIKVGKSASLAVFSEDLCTD
                     DWQKVMAAEVIATYLDGDLVYKK"
     gene            426..1826
                     /gene="yfcC"
                     /locus_tag="IV334_002796"
     CDS             426..1826
                     /gene="yfcC"
                     /locus_tag="IV334_002796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative basic amino acid antiporter YfcC"
                     /protein_id="HAP8944799.1"
                     /translation="METMKRKFTFPHSYTLIFILIILAAILTWIIPSGQFETVVENVA
                     GMEKTTVVPGTYHQIDKADYGQGFSSILMAPAEGIISAVEVVAFVLIVGGAFGIILKT
                     GAIDRGLFTLAKVLGDKGILVIPMSMILFSLGGSTFGMSEEVIPLFAVFISLMFSLGY
                     DSMTAILILFLSTQAGYVGSTINPFNVLIAQGVAGVYGNPLLWFRMLSWIVITGISIV
                     FTMLYASRVKKNPLSSIVYESDLKVKHKFQNFDTEKEFSTQDKLIIAGFIVGLGIMIW
                     GIISKGWYMVEISALFLGLGLFAGGIARFSQKEIAEAFVEGCSDFAYAAVIIGLARGV
                     LVILEGGMIIDTILNSLANLLGGLPKFLFTTILLISQVIVSFFVPSSSGAAALTMPVM
                     APLGDLMNVSRDVVVLSNQFGNGLMNIISPTSGVLLAGLAIAEINFSKWFKVGFKIFA
                     ILTLVSAVLLYIATLF"
     gene            1859..>2003
                     /locus_tag="IV334_002797"
     CDS             1859..>2003
                     /locus_tag="IV334_002797"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HAP8944800.1"
                     /translation="MIKINQLAGMNIHYLFYSLKEFFAYQQKIGISSVELWGGAPHFF
                     IDAF"
BASE COUNT          555 a          339 c          432 g          677 t
ORIGIN      
        1 aaaaatgttt ccacagagga cttctttaat tattgtcgat tagaggaaag tggtgtaaca
       61 gtcactagcg gtacagattt accgctgttt attactgata ttcctgaatc gattataaga
      121 gcagtattcc gtcgttttcc tgaaggagaa gaagtctggg agaaagaaac aagcttcacg
      181 acaaccgagc tgctgaaaag ttttacaagc aacagcttca aagtaaatgg tttatcggag
      241 tacggagaaa taaaagtagg gaaaagcgcc agcttagctg ttttcagcga ggacctctgt
      301 actgatgact ggcaaaaagt gatggctgcg gaagttatcg ctacctatct tgatggtgac
      361 ttagtttaca agaaataatg cttttcgata taacgttata aaacgaatta aagagaggtt
      421 aaaaaatgga aacaatgaaa agaaaattca ccttcccaca tagttataca ttgattttta
      481 tcttaattat tttggctgca attttaactt ggataattcc tagtggtcag tttgagacag
      541 ttgttgaaaa cgtcgcaggt atggaaaaaa caacggttgt tccgggaacc tatcaccaaa
      601 tcgataaggc agattatgga caaggttttt cctctatact aatggcacca gctgaaggga
      661 ttatttcagc tgttgaggtt gttgcttttg ttctcatcgt tggtggtgcc ttcgggatta
      721 tcctaaaaac gggagccatc gatcgagggc tgtttacctt agcaaaagtt ctgggagaca
      781 aggggatctt agttattccg atgtcgatga ttctattcag cttaggagga tcaacctttg
      841 gcatgagcga ggaagtcatt cccttatttg cggtgtttat ttctctgatg ttttctcttg
      901 gctatgattc catgactgcg atcttgattc tatttttaag tacacaagcg ggatatgttg
      961 ggtcgacgat taatcccttc aacgttttaa tcgcacaggg agtagctggc gtttacggaa
     1021 atcccctgct ttggtttcgg atgctatcct ggattgtcat tactgggatt tcaattgttt
     1081 tcaccatgct ttatgctagc agagtcaaaa agaatccact gtcctctatt gtttatgaga
     1141 gtgatttaaa ggttaagcat aagtttcaaa attttgacac tgaaaaagag ttttcaacac
     1201 aagataaact aattatcgcc ggttttatcg taggtttagg aatcatgatt tggggtatca
     1261 tttccaaggg atggtatatg gttgaaattt ctgcactatt cttaggatta ggtctttttg
     1321 ctgggggcat cgctagattt tctcaaaagg aaattgctga agcttttgtc gaaggctgtt
     1381 cagatttcgc ctatgctgct gtaatcattg ggctagctag aggagtcttg gtaatcttgg
     1441 aaggtggaat gattattgac actattctaa acagcttggc taacctttta ggtggtcttc
     1501 caaaattcct gttcactacg attctactca tttctcaagt aattgtttcc ttttttgttc
     1561 catcctcctc aggagctgcg gcattaacga tgccggttat ggcaccattg ggtgatctaa
     1621 tgaatgtaag tcgtgatgtt gttgttctaa gtaaccagtt tggaaatggt ttaatgaata
     1681 ttatctcccc aactagtggt gtattactgg caggattagc catcgcagag attaatttct
     1741 ctaaatggtt taaggttggc ttcaaaatat ttgccatctt aacccttgtt tctgctgttt
     1801 tgttatacat tgctacactc ttttagtctt aatcatccat taataggagg caatagttat
     1861 gattaaaata aatcaattag caggaatgaa tatccactat ttattttatt ctctcaaaga
     1921 atttttcgct tatcaacaga aaattggtat ttcttctgtt gaactttggg gaggtgctcc
     1981 gcattttttc atagatgctt tta
//
LOCUS       DACDKM010000204         1982 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20834_2016
            SAMN12640370-rid9891663.denovo.208, whole genome shotgun sequence.
ACCESSION   DACDKM010000204 DACDKM010000000
VERSION     DACDKM010000204.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640370
            Sequence Read Archive: SRR10040891
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1982)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1982)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742835.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742835.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:04:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,012
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 29 of 75
            Pseudo Genes (incomplete)         :: 33 of 75
            Pseudo Genes (internal stop)      :: 19 of 75
            Pseudo Genes (multiple problems)  :: 5 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1982
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640370-rid9891663.denovo.208"
                     /isolate="E20834_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            273..839
                     /gene="amaP"
                     /locus_tag="IV334_002798"
     CDS             273..839
                     /gene="amaP"
                     /locus_tag="IV334_002798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline shock response membrane anchor protein
                     AmaP"
                     /protein_id="HAP8944801.1"
                     /translation="MNKGLKIIGVIMSLLLLSVFLAIALINAPYIMPEQLERFRFFTL
                     TNYYMQQYIFWAAVVFAVLAIIILLVVLFYPKSRGTFVMKREDGKLTIDKKAIEGLVR
                     SHLHEEEFIHSPKVRIRSTKNRIHIHVNGDLKRTSSLVGKTGVLMQDIEEEVTKVLGT
                     KETVKVAVTYSGYEEQEDTRDYKHSRVE"
     gene            852..1040
                     /locus_tag="IV334_002799"
     CDS             852..1040
                     /locus_tag="IV334_002799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2273 domain-containing protein"
                     /protein_id="HAP8944802.1"
                     /translation="MKELFSAYKLPIIFGGLGLLLAVLWLTIGFFKTLLIIIMTAIGA
                     GIGFYLKQSGIVDQFFKS"
     gene            1053..1610
                     /locus_tag="IV334_002800"
     CDS             1053..1610
                     /locus_tag="IV334_002800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HAP8944803.1"
                     /translation="MENKNMNTTNGSVLANEKGENRMMNGELTYEDKVIQKIIGYALK
                     DVDGLLTLDGGFFSNMAEKLVNTDNVTAGINTEVGKKQVAVDLDVIVEYGKDIEDIYN
                     QIKELISTEVNNMTHLDVIEVNVNVADIKTQEEYQKDQETVQDKVTEAAKSTGQFASR
                     QTDKAKSAVGKGAQKVKENNEPRVQ"
     gene            1642..1884
                     /locus_tag="IV334_002801"
     CDS             1642..1884
                     /locus_tag="IV334_002801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HAP8944804.1"
                     /translation="MGFIWALIVGGIIGAIAGAITNKGSSMGIIANIIAGLVGSAIGQ
                     ALLGTWGPTIAGMAIIPSLIGAIILVLVVSFFARKL"
BASE COUNT          723 a          305 c          406 g          548 t
ORIGIN      
        1 aataaataga atcttgtaat aataactgag cataatcttt gcaattttta atcttgaata
       61 atatccttta agtatagcca gcaatcaaaa gtaaatagag aataagtaag caattgttat
      121 ttcagtatca gactggcaat aaaacgccgt tatgatccag caatgtttgg tagcgcttta
      181 tttactgatg ctatgctaac tgttcgctat ctatactgtg attgtcggta gagttattta
      241 cagggtaaaa ataagtttat aggaggtgga aaatgaataa ggggttaaaa ataatcggtg
      301 ttatcatgag tttactgcta ctttcagtat ttctagcgat tgcgttgatc aatgcgccgt
      361 atattatgcc tgaacaattg gaacgatttc gcttctttac tttaacgaat tactacatgc
      421 aacaatatat tttctgggca gctgtagtat ttgccgttct tgctattatc atcttgctag
      481 tcgttctttt ctatccaaag tcaagaggaa ctttcgttat gaaacgggaa gatggcaaac
      541 taacaatcga caaaaaagcg atcgaagggt tggtacgttc ccatttacac gaagaagagt
      601 tcattcattc acctaaagta cggatacgtt caacgaaaaa tcgaatccat atccatgtca
      661 acggagactt gaaacgaact tcttcattag tagggaaaac aggtgtattg atgcaagata
      721 tcgaagaaga agtaactaaa gtactcggaa caaaagaaac ggtcaaagtg gcagtcacat
      781 acagcggtta tgaagaacaa gaagatacaa gagactacaa gcactctcga gtggaatagg
      841 agggccaaat aatgaaagaa ctattttcag catacaagct ccctattatc tttgggggtc
      901 ttggactact gctagctgtt ttatggttaa cgattggatt tttcaagaca ttgttgatca
      961 taatcatgac agcaattggc gcaggaatcg gtttttattt gaaacagtca ggaatagttg
     1021 atcaattttt taaatcatag gaggaaacaa agatggaaaa caaaaacatg aatacaacaa
     1081 atggatcagt tttggcaaat gaaaaaggag aaaaccgcat gatgaatggt gaattgactt
     1141 atgaagacaa agtcatccaa aaaatcatcg gttatgcatt gaaagatgta gatggtctat
     1201 tgacattaga tggcggattt ttctcaaata tggctgaaaa attagtcaac acagataacg
     1261 taacagcagg tatcaataca gaagtaggta agaaacaagt agctgtggat ctagatgtga
     1321 tcgtagaata cggtaaagac atcgaagata tttacaacca aatcaaagaa ttgatcagta
     1381 ctgaagtaaa caatatgaca catttagatg ttatcgaagt gaatgtgaat gttgcagaca
     1441 tcaaaacaca agaagaatac caaaaagatc aagaaacagt acaagacaaa gtaacagaag
     1501 cagcaaaatc cacaggacaa tttgcttcac gccaaacaga taaagcaaaa tctgctgtag
     1561 gcaaaggtgc acaaaaagtc aaagaaaata acgaaccacg tgtacaataa gaagtaagaa
     1621 gtcagttagg aggaatttaa aatgggattt atttgggcat taatcgtcgg cggtatcatt
     1681 ggagcaattg caggagcaat cacgaataaa ggatcatcaa tggggatcat cgcaaacatc
     1741 attgctggtt tagtaggttc agcaattgga caagctttac taggaacttg gggaccaaca
     1801 attgcaggaa tggctatcat tccatcattg atcggtgcaa tcattttagt attagtcgtt
     1861 tcattctttg cacgtaaatt ataagcaaat tgaagaaaaa cttatgttga aatgaagaac
     1921 aaatttgaga accgtagcga cactcgctgc ggttcttttt tatatatttt atatgaagta
     1981 tt
//
LOCUS       DACDKM010000205         1961 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20834_2016
            SAMN12640370-rid9891663.denovo.209, whole genome shotgun sequence.
ACCESSION   DACDKM010000205 DACDKM010000000
VERSION     DACDKM010000205.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640370
            Sequence Read Archive: SRR10040891
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1961)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1961)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742835.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742835.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:04:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,012
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 29 of 75
            Pseudo Genes (incomplete)         :: 33 of 75
            Pseudo Genes (internal stop)      :: 19 of 75
            Pseudo Genes (multiple problems)  :: 5 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1961
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640370-rid9891663.denovo.209"
                     /isolate="E20834_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(<1..914)
                     /locus_tag="IV334_002802"
     CDS             complement(<1..914)
                     /locus_tag="IV334_002802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase family protein"
                     /protein_id="HAP8944805.1"
                     /translation="MIISTITEAIGSTPLYRFDNQHYSMPKGSAIYAKLEYLNPGGSI
                     KDRLGSYLIKEGFAQGKINEKTTIIEPTAGNTGIGIALAALTYKLRTVFVVPEKFSLE
                     KQQLMKALGAKIIHSPSEQGIIGAISKSKKLAEEISNSYLPLQFENKDNPAAYYHTLG
                     PEIFHEIKENIHSFVAGIGSGGTFAGTSTFLKEKYPDIRIIGVEPEGSVLNGGDPAPH
                     EIEGIGVEFIPPFLSPLSINQIETISDVEGFNYTRQLAREQGLLVGSSSGAAFAAALR
                     EIRRLPPGHRVVTIFPDAADRYLSKNIY"
     gene            1342..1905
                     /gene="efp"
                     /locus_tag="IV334_002803"
     CDS             1342..1905
                     /gene="efp"
                     /locus_tag="IV334_002803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HAP8944806.1"
                     /translation="MISASDLRAGMTFVQDGKLIKVLDASHHKPGKGNTVMRMKLKDV
                     RSGATYDTTFRPEEKFEKAHIDTKPVQYLYTMDDTAFFMDLETYEQYEIPVETVEEEM
                     KYILENMEVKIQFFGSEVIGVQLPTTVVLRVVETQPSIKGATVTGSGKPATMETGLVV
                     NVPDFIETDELLEINTAEGSYVKRASK"
BASE COUNT          651 a          360 c          383 g          567 t
ORIGIN      
        1 aagtaaatgt tttttgataa atagcggtct gctgcgtctg gaaaaatcgt aacgacacga
       61 tgaccaggtg gtaaacgacg aatttctctt aatgctgctg caaaagccgc accactcgaa
      121 cttccaacta atagtccttg ttctcgtgct aactgcctag tatagttaaa cccttctaca
      181 tcagagattg tctcgatctg attgattgaa aggggagaaa gaaacggcgg aatgaattct
      241 acaccgatac cctcaatttc gtgaggggct ggatcaccac catttaacac agagccctct
      301 ggttctacac cgattattcg aatgtcggga tatttttctt tcaaaaaggt agaagttcca
      361 gcaaaggttc caccactccc aattcctgct acaaaactat ggatattttc tttgatttca
      421 tgaaagattt ctggccctaa cgtatgatag taagcagcag gattatcttt attttcaaat
      481 tgaagaggca gatagctgtt tgatatctcc tctgcaagtt ttttactttt gctgatggca
      541 ccgatgattc cttgctcact aggagaatgg atgatctttg ctcccaatgc tttcatcaat
      601 tgttgttttt ctaagctaaa cttctctgga acaacaaaaa ccgttcttaa tttgtatgtg
      661 agtgcggcca gagcgatccc aattcctgta tttccggcag ttggttcgat gatcgtcgtt
      721 ttttcattga tttttccttg ggcaaatcct tctttgatca aataactgcc caatctatct
      781 ttgatacttc ctccaggatt taaatattct agttttgcat aaatagcaga acctttaggc
      841 atagaatagt gctgattgtc aaagcgataa agcggtgtgg aaccaatagc ttctgtaatt
      901 gtcgagataa tcatgtgggc ctcctaaaaa aagataataa aaaaaggagc tgactattaa
      961 atcagctcct tgaaagacat agagaaaaat tgagtagata aaaagataag ttacctggta
     1021 ctcattctat gaagggcaat taatagacat gacaaatagt atgctgcttt tatgcaccat
     1081 acaacagcaa cgattcaatt gtttttggtt gctgactgtc atgtgtatcc ctccaagtaa
     1141 aatttttatt aacgatagca tgaaaaaaaa taaaagtcaa aggcttgaaa gaaaaaaata
     1201 tttcgatagc atcgtttacc cgtgtttatt tttctgacaa ataggcatga gaccttaaaa
     1261 gatagagaat actctagttt tctggccggt tgtctgatac aataaagttg attatgataa
     1321 agaaaagaag gagcaaaaat aatgatctca gcaagtgatt taagagccgg tatgactttc
     1381 gtacaagacg gcaaactgat taaagttctg gatgcaagcc accataaacc tggtaaagga
     1441 aatacagtta tgcgtatgaa actgaaagat gtccgttcag gtgcaacata cgacacaacg
     1501 ttcagaccag aagaaaaatt tgaaaaagca catatcgaca caaaacctgt acaatatttg
     1561 tacacgatgg atgatacagc tttcttcatg gatttggaaa catatgagca atatgaaatt
     1621 cctgtagaaa cagtagaaga agaaatgaaa tatatcctag aaaacatgga agtaaaaatc
     1681 caatttttcg gtagcgaagt catcggcgtt caattaccaa caactgtcgt attacgtgtt
     1741 gtggaaacac aaccttccat caaaggtgca acagtcactg gttcaggtaa gccagcgaca
     1801 atggaaactg gcttagtagt caatgttcct gacttcatcg aaacagatga attgttggaa
     1861 atcaatacag cagaaggttc ttatgtaaaa cgtgcgtcaa aataattcgc taacggattg
     1921 ataagaattt gaaaacagag cttgacctct tgaaaggcag a
//
LOCUS       DACDKM010000206         1957 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20834_2016
            SAMN12640370-rid9891663.denovo.210, whole genome shotgun sequence.
ACCESSION   DACDKM010000206 DACDKM010000000
VERSION     DACDKM010000206.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640370
            Sequence Read Archive: SRR10040891
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1957)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1957)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742835.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742835.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:04:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,012
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 29 of 75
            Pseudo Genes (incomplete)         :: 33 of 75
            Pseudo Genes (internal stop)      :: 19 of 75
            Pseudo Genes (multiple problems)  :: 5 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1957
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640370-rid9891663.denovo.210"
                     /isolate="E20834_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            173..1309
                     /locus_tag="IV334_002804"
     CDS             173..1309
                     /locus_tag="IV334_002804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HAP8944807.1"
                     /translation="MKTIYLDHAATTPMHPQVIEAMTAIMQDTFGNPSSIHGFGRHAH
                     EKLETARQIIADSLQAKPHEIIFNSGGTEGDNTAILETAFSRQKEGRHLITTAIEHPA
                     VLNTMKYLETKGFDVTYLPVDEKGNLSIEEVKKALREDTILVSVMYGNNEIGNLMPIK
                     EIGELLADHPAYFHTDAVQAYGNQTIHPEELGIDLLSISAHKINGPKGVGFLYKRDGV
                     NIPALLLGGEQEEKRRAGTENLAGIWGMAKAVELLSPEEREARNNKYKGFQQLILDTL
                     EANEIPYQVNGDLTNKLPHVLNLRLIGEANDLLLMKLDLNGVAISTGSACTAGNIEPS
                     HVLEAMYGEDTPILKESIRISFGLGNTEDDIQYFLDQLVRAVKK"
     gene            1446..1790
                     /locus_tag="IV334_002805"
     CDS             1446..1790
                     /locus_tag="IV334_002805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1831 domain-containing protein"
                     /protein_id="HAP8944808.1"
                     /translation="MAFETTATVLGAPISYRLHPDAKRYTLRDNGFTETNGGNYQLIR
                     PLDATPQSKEGFKLKITVSKDIQTLKMSITTANGLKAVNIFKDPKQKMSQDKFYFLMD
                     GMISRGLFEKVE"
BASE COUNT          677 a          342 c          408 g          530 t
ORIGIN      
        1 aagaagttgt gacagaagtg agcagcttca aggagctgcg acaagacttt tgtcacagct
       61 ccttttttct atgcccatga aacgtcctct agacaaaaat gagctcttct ttggtttgtt
      121 cgctatttta tgctagaatg tgcctagcaa taatttggaa gggatgaatg aagtgaaaac
      181 tatttatctg gatcatgcgg caactacccc catgcatcca caagtaattg aagcgatgac
      241 ggcaatcatg caagacacgt ttggaaatcc ttcaagcata catggatttg gtcgccacgc
      301 gcatgaaaaa ttggaaacag caagacaaat cattgcagac agtctgcaag caaaacctca
      361 cgagatcatc tttaacagtg ggggaacaga aggggataat acagctatct tagaaacagc
      421 gttctcgcgt caaaaagaag gcagacatct aatcactaca gctatcgaac atcctgctgt
      481 tcttaatacc atgaaatatt tagagacaaa ggggtttgac gttacatatc tgcctgtaga
      541 tgaaaaaggg aatctgtcga tcgaagaggt caaaaaagcg ttacgagaag atacgatctt
      601 ggtctctgtg atgtacggga acaatgaaat cggaaatttg atgccaatca aagaaatcgg
      661 tgaactttta gcggatcatc cagcttattt ccatacagat gcggtacaag cgtacggaaa
      721 ccaaacgatc catccagaag aattaggaat cgatttatta agtatctctg ctcataaaat
      781 caatgggcca aaaggcgtcg gtttcttata taaacgagac ggagtaaata tccctgcatt
      841 gcttttaggc ggggagcagg aagaaaagcg gcgtgcaggt acagaaaatt tagcagggat
      901 ctggggtatg gcaaaagcag ttgaactgct gtcacctgaa gaacgtgaag cacgtaacaa
      961 caaatataaa ggattccagc aattgattct tgatacatta gaagcaaacg aaatccctta
     1021 tcaagtaaat ggtgatttaa cgaataaact ccctcatgtg ttgaatcttc gcttaattgg
     1081 cgaagcaaat gatttgctac tgatgaagct tgatcttaat ggggtcgcta tttctactgg
     1141 ttcagcttgt acagcaggaa atatcgagcc ttcacacgta ttggaagcaa tgtatggaga
     1201 agatacaccg atactaaaag aatccatccg tatcagcttt ggtttgggaa ataccgaaga
     1261 cgacatccag tatttccttg atcaattagt tcgtgctgtc aaaaaataac tagtgaagtc
     1321 attcttatat gaacagatgt ctgagagaac gccttcattt tttagggaac ttaactgaaa
     1381 aacctagtaa aaatcaggat aaacttttat aataaagaga aactaatttt atcgaggtga
     1441 aaatcatggc atttgaaaca acagcaacgg tactgggcgc accaattagc tatcgtttgc
     1501 atcctgatgc aaaaagatat acattgcgcg ataatggatt tacagaaaca aacggaggca
     1561 attatcaatt gatccgacca ttagatgcca cgccgcaaag caaagaagga ttcaaattaa
     1621 aaatcactgt atcaaaagat attcaaacat tgaaaatgtc gatcactaca gcaaatggat
     1681 tgaaagcagt caatattttc aaagatccaa aacagaaaat gagccaagat aaattttatt
     1741 ttctgatgga tgggatgatt agccgtgggt tattcgaaaa agtcgaatga tctgtggata
     1801 taaaaagaca actagttttg aaacggccta ttgttagatg actaactttg taaggacgac
     1861 gcaaattagt tgtctttttt atttttaaat ttaacacatg ttgttatttt taatattttg
     1921 ctgatttatt gtttctaagt aaagaaattt aaattga
//
LOCUS       DACDKM010000207         1921 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20834_2016
            SAMN12640370-rid9891663.denovo.211, whole genome shotgun sequence.
ACCESSION   DACDKM010000207 DACDKM010000000
VERSION     DACDKM010000207.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640370
            Sequence Read Archive: SRR10040891
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1921)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1921)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742835.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742835.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:04:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,012
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 29 of 75
            Pseudo Genes (incomplete)         :: 33 of 75
            Pseudo Genes (internal stop)      :: 19 of 75
            Pseudo Genes (multiple problems)  :: 5 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1921
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640370-rid9891663.denovo.211"
                     /isolate="E20834_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            219..785
                     /locus_tag="IV334_002806"
     CDS             219..785
                     /locus_tag="IV334_002806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAP8944809.1"
                     /translation="MRRKVYTKDQILKAAYDVVAKEGFKGFTARNIAKKMGISTQPIY
                     LEFKNMEDLKNTLLDSIFEQLKEDVFPIERTGDKIIDLGLNYIYFAKKHHSLYMALYI
                     DEHGDGKKMHDFSYNYFREIVKEDERYNQLSDEYVDALHTGTWVAVTGLASLMSSNIM
                     HPTEEQIIALIKRTIDSILALDNPEIIR"
     gene            930..1017
                     /locus_tag="IV334_002807"
     tRNA            930..1017
                     /locus_tag="IV334_002807"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:962..964,aa:Ser,seq:cga)
     gene            1351..>1921
                     /locus_tag="IV334_002808"
     CDS             1351..>1921
                     /locus_tag="IV334_002808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="linear amide C-N hydrolase"
                     /protein_id="HAP8944810.1"
                     /translation="MCTAITYATKDHYFGRNFDYEMSYNEVVTITPRNYRFDFRKVKN
                     LDKHYAMIGIAAGVSNYPLYYEATNEKGLSMAGLNFPGNADYKELQEGKDNVAPFEFI
                     PWILGQCSTIDEAKELLATINLVNIDFSEKLPLSPLHWLLADKEKSIVIESMKDGLHL
                     YDNPVGVLTNNPPFDYQLFNLNNYRSLSNG"
BASE COUNT          680 a          287 c          368 g          586 t
ORIGIN      
        1 gaaatgaaag atttggacaa ctatcattta ggtcaaaact aaaaatgtaa gggaacccat
       61 tgaatttaac caaatttttt taattttgca aaaaaaagtt aaagaagact gaaaaaaaat
      121 ttaaaatata acgttttgag taaaatgttt gcaaaaaatg ctaaaaatag ggtacaataa
      181 aacaacatat actgcagtaa agaagtgggg gagttttaat gagaagaaaa gtctatacca
      241 aggatcaaat tctaaaagct gcttatgatg ttgttgcaaa ggaagggttt aaaggattta
      301 ctgcgagaaa tattgctaaa aagatgggga tttcgacaca gccgatttat ctggaattca
      361 aaaacatgga agatttaaaa aatacgttgc tggattctat ctttgaacaa ctaaaagaag
      421 atgttttccc tattgaacgt actggtgata aaatcattga tctcggtttg aactatatct
      481 actttgcaaa aaagcatcat agtctttaca tggcattgta cattgatgaa catggggacg
      541 gaaagaaaat gcatgatttt tcatacaatt atttccgtga aatagttaaa gaagatgaaa
      601 gatacaatca attatcggat gaatatgtcg atgctctgca tactggtaca tgggtagcag
      661 tgactggctt agcgtcattg atgtcatcaa acatcatgca tccaacagaa gaacaaatca
      721 ttgcactgat caaacggaca attgactcga ttttagcatt agataatcct gaaataatca
      781 gatagtgttc tatcagtatg aatttgatcg gtaagtccgt tggctgcaaa ctgtcaatgg
      841 acttttttgc taaaaaaagc gatttctgtt atttatctat tagaaatagg cttgacattt
      901 cgtgaaaaaa gtttaaaaat agtacttacg gaaggttggc agagtggtaa tgcaccggac
      961 tcgaaatccg gcgaaccggc taataccggc gcgcaggttc aaatcctgta ccttccttat
     1021 atcaacgatt agaggttgtg aagaagctgt cctgcatcaa gtaataagaa cggccaaaca
     1081 aaaatagctt ttcctatttt ttcgtttgat tgggcttatt accgcagatg caagcttttg
     1141 aacaccgttt gttcggaata aggggctgtg acaagacttt tgtcacagcc ccttattgtt
     1201 gtacttttaa cctacaatat tttttgattc cccctgagta ctatttttta taaatgctag
     1261 taaatcaatc ttctttggcc tttttttgat aaaaaaaata ctagatgtaa gataaaagtg
     1321 taaaaacgat ttgttttagg aggggaaacg atgtgtaccg caatcactta tgcaacgaaa
     1381 gaccattatt ttggaaggaa ttttgattat gagatgtctt ataatgaagt ggtcactatt
     1441 actccaagaa attatcgttt cgattttaga aaggtaaaaa atttagataa acattatgca
     1501 atgataggaa tagcagctgg ggtatccaac tatccactct attatgaggc tacaaatgaa
     1561 aaagggttga gtatggcagg actgaatttt cctggcaatg ctgattataa agaattacaa
     1621 gaaggaaaag ataacgttgc accatttgag tttattccat ggatactagg gcaatgctca
     1681 accatagacg aagctaaaga attattggct actatcaatc tagtcaatat cgattttagt
     1741 gaaaaactac ccttgtcacc tttacactgg ttattagctg ataaagaaaa atcgatagta
     1801 attgaaagta tgaaagatgg acttcattta tatgataatc ctgtcggtgt gttgacaaat
     1861 aatccgccat ttgattatca attatttaat ctgaacaatt atcgctcgct atcaaatgga
     1921 a
//
LOCUS       DACDKM010000208         1920 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20834_2016
            SAMN12640370-rid9891663.denovo.212, whole genome shotgun sequence.
ACCESSION   DACDKM010000208 DACDKM010000000
VERSION     DACDKM010000208.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640370
            Sequence Read Archive: SRR10040891
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1920)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1920)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742835.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742835.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:04:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,012
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 29 of 75
            Pseudo Genes (incomplete)         :: 33 of 75
            Pseudo Genes (internal stop)      :: 19 of 75
            Pseudo Genes (multiple problems)  :: 5 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1920
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640370-rid9891663.denovo.212"
                     /isolate="E20834_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            68..1534
                     /locus_tag="IV334_002809"
     CDS             68..1534
                     /locus_tag="IV334_002809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAP8944811.1"
                     /translation="MNNKQLRWFTVALIAFNMVWGMGNVVNNYAQQGVSVVTSWLLIL
                     AIYFIPYALIVGQLGSAFKDSKGGVSSWVENTTSNKRLAYYAAWTYWVVHIPYLAQKP
                     QAILIAGGWAVQGNGNIVNTMSVQMVAVISLIIFLAFLYLSTKGLSTLKVIGGLAGTA
                     MFVMSILFILLAVTAPFINPTMEFATPHMNQVKTYIPTFDFSYFTTVSMLVFAVGGAE
                     KISPYVNSTRNPAKEFPKGMIFLAAMVGICAVLGSVAMGMLFASDNIPKDLMANGAYS
                     AFQILGNYYGIGNSLMIIYALTNMIGQISALAFSIDAPLKILLADADPEFVPNWLRKR
                     NRKGTLVNGYLLTGILVGIIILLPIFGIKDMNELVKWLTNLNSVVMPMRYLWVFLAYM
                     MLNKAYKKFNSEYKFVKNPKIGFIFGAWCFAFTAFACILGMVPKVDYTADPKAWWFQL
                     FTNIITPIVLILLGMILPAIARRDKNKKITEPETVVQP"
BASE COUNT          591 a          328 c          394 g          607 t
ORIGIN      
        1 taagtttgtt aaaaattgaa tatgattaca tctggataac aaatttttat cataaaggag
       61 agaacgaatg aacaacaaac aattacgctg gtttactgtt gcgttaattg catttaacat
      121 ggtttggggc atggggaatg tagttaataa ctatgctcag caaggagtat cagttgtcac
      181 ctcttggcta ttgattttgg ccatttattt tattccatac gcattgatcg ttggacaatt
      241 aggatcagcc ttcaaggata gtaaaggagg cgtcagttca tgggtcgaaa atacgacatc
      301 taataaacga ctggcatact atgccgcttg gacttactgg gtcgtccata ttccttattt
      361 ggcacaaaag cctcaagcaa ttctgattgc tggtggttgg gcagtccaag gaaatggaaa
      421 tatcgtaaat acgatgtcag tacaaatggt agcagttatt tcacttatta tatttcttgc
      481 ctttttatat ttatcaacaa aaggactttc tactttgaaa gtgattggcg gactggccgg
      541 aacagcgatg ttcgtcatgt cgatcttatt tattctttta gctgtaacgg caccgttcat
      601 caatccaacg atggaatttg ccacaccaca tatgaatcaa gtgaaaacat atattccaac
      661 atttgatttc agttatttca caaccgtttc gatgctagta tttgcagttg gaggggcgga
      721 aaaaatatcc ccttatgtca actctacaag aaatcctgca aaggaatttc ctaaagggat
      781 gatcttctta gcagcaatgg tagggatttg tgctgtatta ggctcagtcg caatgggtat
      841 gctgtttgct agtgataata ttcctaaaga cttgatggca aatggggcat acagtgcttt
      901 tcaaatttta ggtaattact atggtatcgg taactcattg atgatcattt acgcattgac
      961 aaacatgatt ggacaaatct ccgcattagc cttttcaatt gatgcaccac taaaaatctt
     1021 gttagcagat gcggatccag aatttgtacc aaactggcta cgtaaacgta atcgtaaagg
     1081 tacattggtg aatggttatc ttctgacagg tatactagta ggaatcatta ttttgttgcc
     1141 gatttttggt atcaaagata tgaatgaatt agtaaaatgg ttgacgaact tgaactcagt
     1201 tgtcatgcca atgcgttacc tatgggtgtt cttggcatat atgatgttga ataaagctta
     1261 taagaaattc aattctgaat ataaatttgt taaaaatcca aaaatcggtt ttatctttgg
     1321 tgcttggtgc tttgccttca ctgcgtttgc atgtatccta ggaatggtgc caaaagtaga
     1381 ttatactgct gatcctaaag catggtggtt ccaattattt acgaatatca tcacaccaat
     1441 cgtcttgatc ttattaggta tgatcttgcc agcaattgct cgtcgagaca aaaacaaaaa
     1501 aatcactgaa ccagaaacag tggttcagcc ttaaaataga aatacttgtt acaagaggtt
     1561 gtgacagaag tggtcagctt caagaaataa gaaggaattt acgaaaattg cttctcaaat
     1621 ttttgtgaag tttcggcttg tttttcgaag aagctacttc tggaacaccg tgaatcagag
     1681 gttgtgaaaa agctgtcctg catcaagtaa taagaacagc caaacaaaaa tagcccctca
     1741 tatttttcgt ttggctgggc ttattaccgc agatgcaagc tttggaacac cgtttaagga
     1801 agaggttgta aaatcagcga tttgacctga gtattagaga aacaaacgga aaaatagctc
     1861 ctcatatttt gccgcttttt gagctaatgc tgaaggtcag ctgattgaac accatttatt
//
LOCUS       DACDKM010000209         1915 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20834_2016
            SAMN12640370-rid9891663.denovo.213, whole genome shotgun sequence.
ACCESSION   DACDKM010000209 DACDKM010000000
VERSION     DACDKM010000209.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640370
            Sequence Read Archive: SRR10040891
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1915)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1915)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742835.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742835.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:04:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,012
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 29 of 75
            Pseudo Genes (incomplete)         :: 33 of 75
            Pseudo Genes (internal stop)      :: 19 of 75
            Pseudo Genes (multiple problems)  :: 5 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1915
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640370-rid9891663.denovo.213"
                     /isolate="E20834_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..153
                     /locus_tag="IV334_002810"
     CDS             <1..153
                     /locus_tag="IV334_002810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
                     /protein_id="HAP8944812.1"
                     /translation="NKFYQSLRTASSTIKGMETIRGIYKKNRRNGTLFSFSVSTEIKV
                     LMGITA"
     gene            159..575
                     /locus_tag="IV334_002811"
     CDS             159..575
                     /locus_tag="IV334_002811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8944813.1"
                     /translation="MEFREGAFDFQTSQQNRKFIPEDFQNLQLENMRLEDLFRLADDD
                     KYRFEVLKIKKNELLNKLYPENAIKAHFGDANHMYSWFIPKRFRGKINAGDQVKVLNR
                     NSTDVVMITDIYVEDEVIIKYMKPVIKLVNKNKGNG"
     gene            681..944
                     /locus_tag="IV334_002812"
     CDS             681..944
                     /locus_tag="IV334_002812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HAP8944814.1"
                     /translation="MAKTVLNPSSARKNFYKLLKDVNENHTEIEIISERSENNAVLIG
                     LDDWKAIKETLLLEQIGTLGVVRKREKDDSGFTNVDEIDWDNL"
     gene            944..1216
                     /locus_tag="IV334_002813"
     CDS             944..1216
                     /locus_tag="IV334_002813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HAP8944815.1"
                     /translation="MNNYSVMIKNSAKADLKKIKQSNLKPQFEKVIQTLKEDPYLPTQ
                     SFEKLKPTHEGRYSRRLNRQHRVVYKVDEDEHVVEIYSARTHYESQ"
     gene            1293..1622
                     /locus_tag="IV334_002814"
     CDS             1293..1622
                     /locus_tag="IV334_002814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HAP8944816.1"
                     /translation="MITKTRKQGNSIMLTVPKDFNVPNGVEVEAKLVENGILYEFVEP
                     QKEFYDFSEDILSDIIAEGYDKDEILVEFKNRKNKMHSSFRDIAEDTLTNSKVMTKEE
                     LATEIGL"
     gene            1612..1898
                     /locus_tag="IV334_002815"
                     /pseudo
     CDS             1612..1898
                     /locus_tag="IV334_002815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292679.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
BASE COUNT          727 a          235 c          381 g          572 t
ORIGIN      
        1 aataaatttt atcaaagtct ccgtacagcc tcttccacga ttaagggcat ggagaccatt
       61 cgaggaatat ataaaaagaa ccgaagaaat ggaacgctct tcagcttttc ggtgtctact
      121 gaaatcaagg tattaatggg aataacagcc taagatattt ggagttcaga gagggcgcgt
      181 ttgattttca aacttcgcaa cagaaccgaa aatttatacc ggaagatttt caaaacttac
      241 agttagaaaa catgcgttta gaagatttat ttagattagc agatgatgat aagtatagat
      301 ttgaagtttt gaaaattaaa aagaatgaac tattgaataa gttatatcct gagaatgcga
      361 taaaagcaca ttttggtgat gctaatcata tgtatagttg gttcattcct aaaagattta
      421 gagggaaaat caatgctgga gatcaagtga aagtactcaa tcgaaatagt acagatgtcg
      481 taatgataac agatatctac gtagaagatg aagttataat caaatatatg aagcctgtga
      541 taaaacttgt taataaaaat aaaggaaacg gataacactt tacaatgctg ttttggtact
      601 ccaatttaaa caaaacaatc gaatttacgt acttaattaa gtacgttaga atggagatat
      661 aaattagagg gggagtagat atggcaaaaa cagtcttaaa tcctagctca gcacggaaaa
      721 atttttataa gttgttgaaa gatgtaaatg agaatcatac ggaaattgaa atcattagtg
      781 agcgcagtga aaataatgcg gtgcttattg gtttggatga ttggaaagca atcaaagaaa
      841 cgctattact tgagcaaata ggcactttgg gtgttgttcg taagcgagaa aaagatgata
      901 gtggatttac taatgttgat gaaattgatt gggataactt ataatgaata actattctgt
      961 gatgattaaa aactcggcta aagctgattt aaagaaaatc aaacagtcta atttgaaacc
     1021 tcaatttgag aaggttattc agactttaaa ggaagatccc tatctcccta cccaatcatt
     1081 tgagaagtta aaacctacac atgaaggtag gtattctaga agattgaata gacaacaccg
     1141 ggttgtttat aaggtagatg aagatgaaca cgtggttgaa atttattcag caaggacgca
     1201 ttacgagtca cagtaattac ttgtaaatac attatctcgt ggtataatgt atttactaag
     1261 taattacata ctgttagaaa tgagggattt aaatgattac aaaaacaaga aaacaaggca
     1321 attcaatcat gttgacagta cctaaagatt ttaatgttcc taatggagtc gaagttgaag
     1381 caaagttggt agagaatggt attctttatg aatttgttga accacaaaaa gaattttatg
     1441 attttagcga agatattttg tctgatataa ttgcagaagg ctacgataaa gatgagattc
     1501 ttgtagaatt taagaataga aaaaacaaaa tgcattcttc ctttagagat attgctgaag
     1561 atacacttac taattctaaa gtaatgacta aagaggagtt ggcaacagaa attggcttat
     1621 gaaatcgttc cttcaaaaca tgtcattaag tatttaaaaa gctaaaagag aagccattga
     1681 aagaaaaatt tttgaattta atttatgatg aaattgcgat tgatccatat aagggtgaac
     1741 aaaaaactgg ggatttatca ggtatttggt ctagtgggtt caaatatgcg ggcacaacat
     1801 atagagtagc ttacgagatt atagaaaaca aggttattcc agtattgtta tgtggaacgc
     1861 atgaaaactt ttatgaacag ttgaaaaaat taatctagtg atttgttgtc gttct
//
LOCUS       DACDKM010000210         1893 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20834_2016
            SAMN12640370-rid9891663.denovo.214, whole genome shotgun sequence.
ACCESSION   DACDKM010000210 DACDKM010000000
VERSION     DACDKM010000210.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640370
            Sequence Read Archive: SRR10040891
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1893)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1893)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742835.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742835.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:04:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,012
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 29 of 75
            Pseudo Genes (incomplete)         :: 33 of 75
            Pseudo Genes (internal stop)      :: 19 of 75
            Pseudo Genes (multiple problems)  :: 5 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1893
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640370-rid9891663.denovo.214"
                     /isolate="E20834_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..177
                     /locus_tag="IV334_002816"
     CDS             <1..177
                     /locus_tag="IV334_002816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002570387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8944817.1"
                     /translation="QSLVLLAIVVIGLFLGFKREFSKLIGFLVIALIAVGLVFNASGV
                     KDVLLNLFNRIVGA"
     gene            190..690
                     /locus_tag="IV334_002817"
     CDS             190..690
                     /locus_tag="IV334_002817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HAP8944818.1"
                     /translation="MEQIRVYIANLGKYNEGELVGAWFTPPVDFDEVKERIGLNDEYE
                     EYAIHDYELPFEIDEYTPIEEINRLCGLAEELKGTPIGEVASEIQHAFFNSFEEMVEH
                     VDDIIYYPDCNDMSDVAYYLIVEAGDLGEVPEHLKTYIDFDACGRDLEIGGNYLVTSR
                     GVFELM"
     gene            787..>1893
                     /locus_tag="IV334_002818"
     CDS             787..>1893
                     /locus_tag="IV334_002818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HAP8944819.1"
                     /translation="MVGNKWYKCDLHIHSTASQCFQDKKVTAKEWVEECLNKGLDCVA
                     LTDHNTGENIDEYKKVAIENGLDFFPGVELTCGENGTHLLVLFDKSACTNDISDFLTK
                     MGIKREDWASAAACSMKSVLEVIDFAEHEGLLVIPAHIDEFNGISSAKFGNLGKIFES
                     ENIYAVQTVQKEFFENRSQMIPSSKRDTIYDSVNARYDGRVGKDTLESWYKSVVEAEK
                     NNMTFLSFSDNPHSKGNSKHGLWGIGTRYSYIKMKDEPDLGSLRDALMLGETRVHSDF
                     SNFSINENEVLLEKLSFSGTTLSTKEVVVEFSDNLTSIIGGRGTGKSCITRFLVYVLG
                     KEAELDQFSEIQSDYQNFAQIEHNGSGIFLKDTIV"
BASE COUNT          592 a          261 c          434 g          606 t
ORIGIN      
        1 caatctttag tcttactagc gattgtggtc attggtttgt ttttaggatt taaacgcgag
       61 ttttccaaac tgattggctt cttggtgatt gctttgattg cggtaggtct ggtctttaat
      121 gcttctggtg tgaaggatgt cttactcaat ttgtttaacc gaattgtcgg tgcctgaggg
      181 ggaatcacaa tggaacagat acgtgtttat attgcgaacc ttggaaaata caatgaaggt
      241 gagttggtgg gggcatggtt taccccaccc gtcgattttg atgaagtgaa agaacggatt
      301 ggattaaatg acgaatatga agagtacgca attcatgatt atgaactgcc ttttgagatt
      361 gatgagtata caccaattga ggaaattaat cggttgtgtg gtttagcgga agagcttaaa
      421 ggaacgccaa ttggtgaggt cgcttcagag attcaacatg ccttcttcaa ttctttcgag
      481 gaaatggtag agcatgtgga tgatatcatc tattatcccg attgtaatga catgagtgat
      541 gtagcgtatt acttgattgt agaagctggt gatttaggcg aagtaccaga acacttgaag
      601 acgtatattg attttgatgc gtgtggtcga gacttggaga ttggcgggaa ttatttggtt
      661 acgagtcgag gggtttttga actaatgtaa atcatttgtt agaattgacc agaaatttct
      721 atcgtacttt tacttttcta tttgatatac tgaaatagaa aatatttgtt gataggggtg
      781 ttactaatgg ttggcaataa gtggtacaaa tgtgatcttc atattcattc aactgcgagt
      841 cagtgctttc aggataaaaa ggttactgct aaggaatggg tagaagaatg tttaaacaaa
      901 ggactcgatt gtgttgcatt aactgatcac aatacagggg aaaatattga tgaatataag
      961 aaagtggcta tcgaaaatgg cttagatttt tttcctggag tggaattaac gtgtggagaa
     1021 aatggaactc atttactagt cctatttgat aaaagtgcat gtacaaatga tatctctgat
     1081 ttcttaacca agatgggtat aaaaagagag gattgggcca gcgctgcagc ttgctcaatg
     1141 aaatcagttt tagaagttat tgattttgct gagcacgagg gcttgctcgt tattcctgca
     1201 catatagatg agtttaatgg aatttcttca gcaaaatttg gaaatctagg aaagatattt
     1261 gaatcagaaa atatatatgc agtacagacg gttcaaaaag aattttttga aaatcgctca
     1321 caaatgattc cctcatctaa gcgtgacact atctatgata gtgtgaatgc cagatatgat
     1381 ggtagagttg gaaaagatac tttggaatct tggtataaat cagttgttga ggcagaaaaa
     1441 aataatatga catttcttag cttttctgat aacccacact cgaaaggaaa ttcaaaacat
     1501 ggactttggg gaataggcac aagatattcc tatataaaaa tgaaggacga acctgacttg
     1561 ggatcacttc gcgatgcttt aatgctaggc gaaactcgag ttcattctga tttttctaat
     1621 ttctccatta atgaaaatga agttttattg gaaaaattaa gcttttctgg gaccactctt
     1681 agcacaaaag aagttgtagt cgaattcagc gataatttaa cttcaattat tggaggaaga
     1741 ggaacaggca aatcttgtat tacaagattc ttggtctatg tccttggaaa ggaagcagaa
     1801 cttgatcaat tttctgaaat acaaagtgat tatcagaact ttgcacaaat tgaacataat
     1861 ggctctggaa tatttttgaa ggatacaatt gtg
//
LOCUS       DACDKM010000211         1884 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20834_2016
            SAMN12640370-rid9891663.denovo.215, whole genome shotgun sequence.
ACCESSION   DACDKM010000211 DACDKM010000000
VERSION     DACDKM010000211.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640370
            Sequence Read Archive: SRR10040891
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1884)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1884)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742835.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742835.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:04:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,012
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 29 of 75
            Pseudo Genes (incomplete)         :: 33 of 75
            Pseudo Genes (internal stop)      :: 19 of 75
            Pseudo Genes (multiple problems)  :: 5 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1884
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640370-rid9891663.denovo.215"
                     /isolate="E20834_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(<1..192)
                     /locus_tag="IV334_002819"
     CDS             complement(<1..192)
                     /locus_tag="IV334_002819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000470930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAP8944820.1"
                     /translation="MFISTLQIRNFRNYESEIFRFDNETNTIIGENDSGKTNALTALR
                     ILLDDSYYYSSKTLKESDFF"
     gene            complement(326..1516)
                     /locus_tag="IV334_002820"
     CDS             complement(326..1516)
                     /locus_tag="IV334_002820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element ISLgar5 family transposase"
                     /protein_id="HAP8944821.1"
                     /translation="MNDFTTEILKTLANKGDLNELFRVHLEKAVNTLLKTELTAFLDY
                     EKYDRIGFNTGNSRNGSYDRTVKTEYGELHLQIPRDRNGEFKQQTVPAYRRTNDTLEE
                     TVIHLFRKGITMSEIADLIEKMYGHYYTPQTMSNITKSFTEEVTAFNGRELHDRYAAI
                     YMDATYIPLKRKTVAKEAIHIAVGIRPDGSKEVLSYAIAPTESITIWEEILLDLQERG
                     LKNVLLFITDGLKGMVGAISRFYPKARFQHCCVHVSRNISHKVRVDDRKEVCDDFKMV
                     YQASSKEVALEARGTFAEKWKTSYPKVVESILSNDHLLTFYDFPLAIRKSIYSTNLIE
                     SFNKQIKKYSHRKEQFQNEESMERFLVSSFDTYNQKFLGRSHKGFQQAEGELEQMLSQ
                     LIEN"
BASE COUNT          544 a          369 c          340 g          631 t
ORIGIN      
        1 aaaaaaatct gattctttta gtgttttact actatagtaa taactatcat ctaataagat
       61 tctcaatgca gtaagagcat tggttttccc tgaatcattc tcacctataa tagtatttgt
      121 ctcattatcg aatcgaaaga tttcggattc ataatttcta aaatttcgaa tttgaagagt
      181 agatataaac ataagcagat ctcctaacta tttaattaat atgattatat agtaagttta
      241 tcataaatta tgttagtaaa agttctcatt ataagggagc gtcaataatt ttgtgtaaat
      301 gattcttccc tactgcagat tgtttctaat tctcaatcag ttggcttagc atttgttcga
      361 gctcgccttc ggcctgttgg aaacctttat gactgcgacc taggaatttt tggttgtaag
      421 tatcaaaaga cgagactaag aaacgttcca tcgactcttc attttggaac tgttccttgc
      481 ggtggctgta tttcttgatt tgtttattaa aggattcaat caagttcgta gaataaatac
      541 tcttgcgtat ggccaagggg aaatcataga aagtgagcaa gtgatcgttc gaaagaatcg
      601 attcaaccac ttttggatag ctggttttcc atttttccgc aaaagtacca cgtgcttcca
      661 acgccacctc tttagatgag gcttgataca ccattttaaa atcatcacag acttccttac
      721 gatcatcgac acgcacttta tgactgatat tacgggaaac gtgtacacaa caatgttgaa
      781 aacgagcttt gggatagaac cgactaatcg ctcctaccat cccctttaag ccatccgtga
      841 tgaacaggag gacatttttc aaaccgcgct cttgaaggtc caataaaatt tcctcccaaa
      901 tcgtgatgga ttcagtcggt gcaatcgcat agctcaatac ttcctttgat ccgtccgggc
      961 gaatgccaac tgcgatatga atagcttcct tggcgacggt tttccgcttt aacggaatat
     1021 acgttgcgtc catataaata gcagcataac ggtcatgaag ctcccgccca ttaaacgccg
     1081 ttacctcttc tgtaaatgat tttgttatat tggacatggt ttggggcgtg tagtaatgcc
     1141 catacatttt ctcaatcaag tctgcgattt ccgacatggt aatacctttt cggaagaggt
     1201 gaatgacggt ctcctctaac gtgtcattcg tccgtctata agcaggaaca gtctgttgct
     1261 tgaactcacc attgcggtcg cgcggaatct ggagatgaag ttccccatac tcggtcttga
     1321 ccgtacggtc ataggagccg ttacgagaat tacccgtgtt aaaaccaatg cgatcatact
     1381 tttcgtaatc taggaaagcc gttaactccg ttttgagaag cgtattgact gctttctcca
     1441 aatggacacg gaataattca ttcaaatcgc ctttgttcgc tagagtcttt agaatttctg
     1501 tagtaaaatc gttcataggg aagtcctctc ttctgtgaat gtgttgtggt aactctattc
     1561 tacagaaggg acttcctttt ttctatttac acaaaatatt ttacactctc cattataaca
     1621 attgggttaa aaagaaaata gatagtattc tacctcatat ttatttgaaa gtaatatata
     1681 aaagacgttc aaattcgaac gcctctcgtt aaccaaatat tggattcaga atctagtttt
     1741 catatataaa cagagtatga ttaggtgcaa aaattgcact tttattgcac ttttattttt
     1801 atttatagtg ttttactgtt ctgtatattg ttttaactcc cttgatagta tcgcattctg
     1861 ttttatacac tttacgttga aaac
//
LOCUS       DACDKM010000212         1835 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20834_2016
            SAMN12640370-rid9891663.denovo.216, whole genome shotgun sequence.
ACCESSION   DACDKM010000212 DACDKM010000000
VERSION     DACDKM010000212.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640370
            Sequence Read Archive: SRR10040891
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1835)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1835)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742835.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742835.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:04:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,012
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 29 of 75
            Pseudo Genes (incomplete)         :: 33 of 75
            Pseudo Genes (internal stop)      :: 19 of 75
            Pseudo Genes (multiple problems)  :: 5 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1835
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640370-rid9891663.denovo.216"
                     /isolate="E20834_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(1..859)
                     /locus_tag="IV334_002821"
                     /pseudo
     CDS             complement(1..859)
                     /locus_tag="IV334_002821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611166.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            1102..1557
                     /gene="tnpA"
                     /locus_tag="IV334_002822"
     CDS             1102..1557
                     /gene="tnpA"
                     /locus_tag="IV334_002822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002366027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
                     /protein_id="HAP8944822.1"
                     /translation="MSNDDKSLAYTRWNCKYHIIFTPKYQRKVIYGQLRKDLGKILRK
                     LCEMKDVEIIEAHAMLDHIHMLVRVPPKICISSFMGYLKGRSAVLIHEQHANLKYKYG
                     NKSFWSKRYYVSTVGLNQKTNEKYIREQEADDRIRYIISKREYVEPFKK"
BASE COUNT          551 a          347 c          357 g          580 t
ORIGIN      
        1 acttgtcctc gataagaatt gtagcgccgt gatttacgtg tgtatgtcgt tacttttatt
       61 cctagcttct tgatcaggcg ctgaactttc tttttattga tagagaaacc gcggtttctt
      121 agttcattgg tcatccgtaa acagccataa tccttgtgct tctcccgaac tttaagcatt
      181 tccgcttcaa tctcttgatc tggattttta cggtccaatc gtttttgcca gtacataaag
      241 attgccttag ggaactttaa agtttctaga agctcaacta atttaaacgg tcctcggagg
      301 cttgcgatga ttcgtgcgat tgcttcattc gtcgttgttg ggcttcctgt tttcgcagct
      361 tcctcaattc ttttaaaaag gcattttcaa tttgaaggga acggactcgt ttctccaact
      421 cctcgatgcg ttcacgttct ttagacgttg ctttagttgt agattttttc ttatttttgg
      481 gttcattttt cttagacaag ataggtggac atcctttcgt ttttgagagt ccatcgatcc
      541 caccctcacg gaattgacgc atccaactag cgatcaaatt agggttgttc atgttaagtg
      601 tgttggcaac ttcacgatag gacagctcac ttgttagata taactctatc gcatccagct
      661 taaattgaac agaatatttt ttcttttgtc gctttcttac tagaccctct tcaccaaatt
      721 cttgataggc attgatccag cgcttcactt gtgatggagc cttaatggaa tatttctttg
      781 ccagataatc acttccgcct ttaccatcca agtaatcatg aacgatcttt aatttaagtt
      841 caaaagtata cttagccata aaaaacaccg acctccaaaa gttagatttt ttggtctaac
      901 ttttgggggt cggtacaaaa cttaaaagtt ttagaccctt ttattttctt aatgacttca
      961 gtcagttttg tgcgtaaagc gtgcgaaaca agaaaaccgc gcgtttctac gcgtggaaga
     1021 agtatcttta gcgataaaaa ctcctttcga tatactagtg agtggctacc aaccgctcat
     1081 cagatcgaaa ggagtttttt catgtcaaat gacgataaaa gtttagcata tactcgttgg
     1141 aattgtaagt atcatattat ttttacacca aaatatcaac gaaaagtaat ctatgggcaa
     1201 ttacgaaaag atcttggaaa aatacttagg aagttgtgcg aaatgaaaga tgtggaaatc
     1261 atagaagcac atgcaatgct agatcatatt cacatgcttg ttcgagtacc accaaaaata
     1321 tgtatatcca gttttatggg gtatctgaaa ggtcgtagtg cggtactaat ccacgagcag
     1381 cacgcaaatc tcaaatataa atacggaaac aaatcctttt ggtcgaagag atactatgtg
     1441 agtacagtcg gattgaatca aaagacaaat gaaaaataca tccgtgaaca agaagctgat
     1501 gatcgcattc gatatattat aagtaaaaga gagtacgtag aaccatttaa aaaataaaga
     1561 gaagtaagtg agtgaagatg agcagttggc ggtcttttaa aaatcccctt ttaaggggca
     1621 gcgagtgtcg accccttcta gggggattaa aaatacaccc gtcctcacgg gtgtatgggt
     1681 tactgataga cttgatttta actggtgtca gaaacttatg tccagaaagg gtgatgaaat
     1741 tttggcaatt tacggctatt caagaattag cacaagcaac caagattaca aaacacaaat
     1801 tcaaacatta gaaaatgctg gcgcagaaat aatct
//
LOCUS       DACDKM010000213         1796 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20834_2016
            SAMN12640370-rid9891663.denovo.217, whole genome shotgun sequence.
ACCESSION   DACDKM010000213 DACDKM010000000
VERSION     DACDKM010000213.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640370
            Sequence Read Archive: SRR10040891
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1796)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1796)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742835.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742835.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:04:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,012
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 29 of 75
            Pseudo Genes (incomplete)         :: 33 of 75
            Pseudo Genes (internal stop)      :: 19 of 75
            Pseudo Genes (multiple problems)  :: 5 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1796
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640370-rid9891663.denovo.217"
                     /isolate="E20834_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            29..1381
                     /gene="brnQ"
                     /locus_tag="IV334_002823"
     CDS             29..1381
                     /gene="brnQ"
                     /locus_tag="IV334_002823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HAP8944823.1"
                     /translation="MTKKLSFKDYLFIGSMLFGLFFGAGNLIFPVHLGQEAGAATFWA
                     NLGFLVTGIGLPFLGVIAIGVSKSSGVYELAKRINQTYAMVFTILLYLVIGPFFALPR
                     LATTSFEIGLAPFIDPSRQTLYLAIFSIVFFVLAWWFSRKPTKILDYVGKFLNPAFLV
                     LLGALLLLAFLNPLGSVNQAPIQPNYQEHAFFTGFTQGYNTLDALAALAFGIIIVTTI
                     QNMGVTKPAEIAKDTIKSGAISIILMGIIYTLLAYLGAMSLGSFALSENGGITLAQIA
                     DHYLGTYGSILLAFIVILACLKTGIGLITAFAETFTDLFPQRNYAFFVALASILPCLA
                     ANVGLTNIIQFSLPVLMFIYPLAMTLILLVLVGPLFKQRTAVYRMTTYFTLIASIFDG
                     LNACPETIKQTPIVQNLLYVAESYLPFFKLGMGWIVPAVIGFVIGLIWSFAKKEEVAD
                     "
BASE COUNT          503 a          309 c          353 g          631 t
ORIGIN      
        1 ttttaaaaaa accgtaaagg agttatgtat gacaaaaaaa ttatcattta aagactatct
       61 ttttataggc tccatgcttt tcggtctatt ttttggtgct ggaaatctta tctttcccgt
      121 tcatttgggt caagaagcag gcgctgctac cttttgggcg aatctaggtt tcttggttac
      181 cggtatcggt cttcccttct taggggtgat tgccattggt gtatcgaaaa gctcaggtgt
      241 atatgaatta gcaaaacgga taaatcaaac atatgcgatg gtttttacca tcttgcttta
      301 cttagttatc ggaccgtttt ttgctttacc tcgccttgca accacatctt tcgagattgg
      361 tttagcacca tttatcgatc ctagcagaca gacgctatat ctagcaattt tcagtattgt
      421 attttttgtg ctggcttggt ggttttcaag aaaaccgaca aagatattag attatgttgg
      481 aaagttcctg aatcctgcat ttttggtttt attaggtgct ttgcttctcc ttgctttttt
      541 gaatccacta ggttctgtaa atcaagcgcc tattcagcca aattatcaag agcatgcttt
      601 tttcacaggg tttactcaag gatataacac attagatgcg cttgctgctt tagcatttgg
      661 gatcatcatt gtcacaacaa tccaaaacat gggtgtaaca aaacctgctg aaatcgctaa
      721 agacacgatc aaatctggtg cgatcagcat tatattgatg ggaatcattt atactttgct
      781 cgcttattta ggtgctatga gcttaggtag ctttgctcta agtgaaaatg gcgggatcac
      841 gctggctcag attgctgatc attatttagg tacttatggc agtatcttat tagcatttat
      901 cgttatatta gcttgcttga agaccggtat tggtttgatt acagcttttg cagaaacatt
      961 cacagatttg tttccgcaaa gaaattacgc attctttgtt gcacttgcta gtatccttcc
     1021 ttgtttagcg gcaaacgtag gcttgacgaa tatcattcaa ttttcgttgc ctgttttgat
     1081 gttcatctat ccattagcaa tgacactgat tttactagtg ttagttggtc ctttgtttaa
     1141 gcagcgtacg gctgtttatc gaatgactac ttattttaca ttgattgctt cgatttttga
     1201 tgggttgaac gcttgcccag aaactatcaa acaaacacct attgttcaaa atcttttata
     1261 tgttgcggaa agctacttgc cgtttttcaa attggggatg gggtggatcg taccagcagt
     1321 tatcggtttt gttatcggac tgatttggag tttcgctaag aaagaagaag tagcagattg
     1381 ataaaagagg ttgtgaaaga gacgttcagc cttgagcaat aaaaagtaat tttgaaaaat
     1441 ggcttctcac atttttgcat aaatttgact taataccgag aaactgactt ttgaacaccg
     1501 tttattgaga ggtcgtgaaa aagccgctta gtttcaagta ttaagaaata ttttgtgaaa
     1561 atggcttctc acatttttgc ataaatttga cttaataccg agaaactggc ttttgaacac
     1621 cgtttattga gcgtctataa actgacctga gtattagaga aacaaaacgg caaagtagct
     1681 ccatatactt tgccgttttg ttttgaacta ataccgaaga gaagctgatc aaacaacatt
     1741 tcttttataa tatagatttt agacatatat atgatataaa aatagttttc gcaaaa
//
LOCUS       DACDKM010000214         1760 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20834_2016
            SAMN12640370-rid9891663.denovo.218, whole genome shotgun sequence.
ACCESSION   DACDKM010000214 DACDKM010000000
VERSION     DACDKM010000214.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640370
            Sequence Read Archive: SRR10040891
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1760)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1760)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742835.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742835.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:04:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,012
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 29 of 75
            Pseudo Genes (incomplete)         :: 33 of 75
            Pseudo Genes (internal stop)      :: 19 of 75
            Pseudo Genes (multiple problems)  :: 5 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1760
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640370-rid9891663.denovo.218"
                     /isolate="E20834_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            122..934
                     /locus_tag="IV334_002824"
     CDS             122..934
                     /locus_tag="IV334_002824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HAP8944824.1"
                     /translation="MLRFFPQKNKYIQEKSEFQFNTDGPIPQHIAIIMDGNGRWAQNR
                     RLPRVAGHKEGMETVKKVTKKASRLGVKVLTLYAFSTENWKRPKDEVSFLMQLPVDFF
                     DTFVPELIKENVKVHVMGYENVLPEHTQDAVRRAIEQTKNNTGMVLNFALNYGSRAEI
                     VTAVQEIAEEVAKGEIHAEEIDDELIAKHLMTGFLPKELQDPELMIRTSGEERISNFL
                     LWQIAYSELYFTKALWPDFDGAHLEEAIASYQNRDRRFGGVKKTEDEKGDQS"
     gene            931..1731
                     /locus_tag="IV334_002825"
     CDS             931..1731
                     /locus_tag="IV334_002825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HAP8944825.1"
                     /translation="MKQRVVTAAVALVLFIPIIWIGGIAVELTAAILAVIGVYELFRM
                     KGLTLLSFEGVLSAVGAVLLVLPKERWFFFLPENVSTFMLFYLIVMILLGTSVISKNT
                     YTIDEAGFPVLVSLYVGIGFQNFVNARTESLAVLIFGLFIVWATDIGAYMVGRRYGQH
                     KLSPDISPNKTIEGALGGIGSAVVVAFLYLLLYPAKELFNHGMFVMLLMTIIFSAVGQ
                     FGDLVESAIKRHYDVKDSGNILPGHGGILDRFDSLLFVFPIMHLFGII"
BASE COUNT          549 a          273 c          394 g          544 t
ORIGIN      
        1 taaaaaaaga aaaaattatt cagtttttaa ataaaaaaac aatgatttca taggaaaaac
       61 agttagatat tgctataata cttaagatag tgggaatctt ttaagtatag gaggaaaaag
      121 aatgttacgt ttttttccgc aaaaaaacaa atatattcaa gaaaaaagcg aatttcaatt
      181 taataccgat ggaccaattc ctcagcacat tgccatcatc atggacggca atggtcgttg
      241 ggcgcaaaac cgtcgtttgc cacgagttgc tggccataaa gaaggcatgg aaacagtaaa
      301 gaaagtcaca aaaaaagcat caaggcttgg agtgaaagtg ttgacgcttt atgccttttc
      361 gactgaaaac tggaagcgac caaaagatga agtaagtttt ctcatgcaac tacctgtcga
      421 cttctttgat acgttcgtac cagaattgat taaagaaaat gtcaaagttc atgtcatggg
      481 ttatgaaaac gtattgccag aacatacgca agatgctgta agacgtgcaa ttgagcaaac
      541 aaaaaacaat actggaatgg tattgaattt tgctttgaat tatggaagca gagcagagat
      601 cgtgactgct gtccaagaaa ttgccgaaga agtagcaaaa ggagaaatac acgcagaaga
      661 aatcgatgat gaattgattg ctaagcattt aatgacaggt ttcttgccta aagagttaca
      721 agatcctgag ctgatgatcc gtacaagtgg agaagagcga attagtaatt ttctgttatg
      781 gcagatcgca tatagtgagc tgtattttac aaaagcgctt tggcctgatt ttgatggtgc
      841 gcatcttgaa gaagcaatcg cctcttatca aaatcgagac cgtcgattcg gcggtgtaaa
      901 aaaaacagaa gatgagaaag gggatcagtc atgaaacaac gtgtggtcac agcagccgtg
      961 gcgctagtct tgtttattcc gattatatgg atcggaggca tagcggttga gcttacagca
     1021 gcaatcttag cagtaatagg agtttacgag ctgtttcgga tgaaaggatt gacactctta
     1081 agttttgaag gagttttatc cgccgttggt gcagtgttac ttgttttgcc aaaagaaaga
     1141 tggtttttct ttcttccaga gaatgtaagt acatttatgt tattttatct tatagttatg
     1201 attcttttag gaacatcagt tatttctaaa aatacgtata cgattgatga agcaggtttt
     1261 cctgtgcttg tcagcttgta tgtgggaata ggtttccaga attttgtcaa tgcacgaaca
     1321 gaaagcttgg ctgttttgat atttggtcta tttattgttt gggcaactga tatcggagca
     1381 tatatggttg gtcgacgtta tggtcagcat aagttaagtc ctgatatttc gcccaacaaa
     1441 acgattgaag gtgcgctcgg tggtattggt agtgctgttg tagtcgcatt tctttattta
     1501 ttgctatacc cagcaaaaga actatttaat catggtatgt ttgtcatgct tctgatgacg
     1561 attatttttt cagcagtcgg acaatttggt gatttggtcg aatctgcaat caaacgtcat
     1621 tatgatgtga aagactcggg aaatatctta ccaggccatg gtggaatctt agaccgtttc
     1681 gacagtttgc tgtttgtatt tcctatcatg catttatttg gcattattta gtaagtatac
     1741 ctgcgtttct ttgtgaattt
//
LOCUS       DACDKM010000215         1735 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20834_2016
            SAMN12640370-rid9891663.denovo.219, whole genome shotgun sequence.
ACCESSION   DACDKM010000215 DACDKM010000000
VERSION     DACDKM010000215.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640370
            Sequence Read Archive: SRR10040891
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1735)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1735)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742835.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742835.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:04:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,012
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 29 of 75
            Pseudo Genes (incomplete)         :: 33 of 75
            Pseudo Genes (internal stop)      :: 19 of 75
            Pseudo Genes (multiple problems)  :: 5 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1735
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640370-rid9891663.denovo.219"
                     /isolate="E20834_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..1350
                     /locus_tag="IV334_002826"
     CDS             <1..1350
                     /locus_tag="IV334_002826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HAP8944826.1"
                     /translation="TNLPALPEGTTVTDVTPGGTIDTNTPGNYEGVIEVTYPDGTKDT
                     VKVPVEVTDNRSDADKYEPTVEGEKVEIGGTVDLTDNVTNLPALPEGTTVTDVTPGGT
                     IDTNTPGNYEGVIEVTYPDGTKDTVKVPVEVTDNRSNADKYNPEGQKVTTDLNKEPDA
                     SEGIKNKEDLPKGTTYAWKEKVDVSTAGNKKGTIVVTYPDGSSDEVEVDVTVTDTRSD
                     ADKYNPEGQKVTTDLNKEPDASEGIKNKEDLPKGTTYTWKEKVDVSTAGNKKGTVVVT
                     YPDGSKEEVEVTISVVDKKAPNKPQVDPITEGDQIVTGKTEPNAEVTVTLPDGSQHHG
                     TADKNGNFTVKVPKLEAGTKVIVTATDESGNTSEPTNVVVSSNKKDSGKNGSKGSKTD
                     NQGSNSNQDKNRGKSQSSKVFPKTGESDSNIFTISGGLILLGTLGLLGYKNRKKENE"
     gene            1484..>1735
                     /locus_tag="IV334_002827"
     CDS             1484..>1735
                     /locus_tag="IV334_002827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HAP8944827.1"
                     /translation="MEKIVIIGSNHSGLAAARFLNLSDSNYEVILIDKKSEISYLECG
                     TSLFIKGEVSNLEDLFYQNRMTLNSEIKKLFLETEVDKID"
BASE COUNT          689 a          254 c          364 g          428 t
ORIGIN      
        1 actaacttgc cagcgttacc agaaggaaca acagtaacag atgttactcc cggcggtaca
       61 attgatacca acacaccagg taattacgaa ggtgtcattg aagtaacgta tccagacggt
      121 acgaaagata cggtgaaagt tccagtagaa gtgacagaca atcgttctga cgccgataaa
      181 tatgaaccaa cagtagaagg cgaaaaagta gaaattggag gcacagtaga tttaacagat
      241 aatgttacta acttgccagc gttaccagaa ggaacaacag taacagatgt tactcccggc
      301 ggtacaattg ataccaacac accaggcaat tatgaaggtg tcattgaagt aacgtatcca
      361 gacggtacga aagatacggt gaaagttcca gtagaagtga cagacaatcg ttctaatgca
      421 gataaataca atcctgaagg acaaaaagtg actacagact taaataaaga gccagatgca
      481 tctgagggaa ttaagaacaa agaagattta ccaaaaggaa ctacttatgc ttggaaagag
      541 aaagtagatg ttagcacagc tggaaataaa aaaggtacta ttgtagtgac atatccagat
      601 ggatcatctg atgaagttga agtagacgtt acagtgacag atactcgttc cgatgcagat
      661 aaatataatc ctgaaggaca aaaagtgact acagacttaa ataaagagcc agatgcatct
      721 gagggaatta agaacaaaga agatctacca aaaggaacta cttatacttg gaaagaaaaa
      781 gtagatgtta gtacagctgg aaacaaaaaa ggtactgtag tagtaacata tccagatgga
      841 tctaaagaag aagtagaagt tactatttct gtagtagata aaaaagcacc gaataaacct
      901 caagttgatc ctattacaga gggtgaccaa attgttactg gtaaaactga accaaatgca
      961 gaggtgacag ttacattacc agacggaagt caacaccatg ggacagctga taaaaatggt
     1021 aattttacag ttaaagttcc taaattagag gcagggacaa aagtgatagt aactgcaact
     1081 gatgaatctg gtaatactag tgaacctact aatgtagttg tttcttccaa caaaaaagac
     1141 agtggaaaaa acggtagtaa aggtagtaag actgacaatc aaggtagcaa cagcaatcaa
     1201 gacaaaaata gaggcaaaag ccagtcctca aaagtttttc ctaagacggg agagagtgac
     1261 agtaatatct ttactattag cggtggttta atactgttag gaacgttagg attattaggg
     1321 tataaaaatc gaaaaaaaga gaatgaatag aaaatcaatt tcttaagtct tctctgagaa
     1381 gaggaagaat ctttttcttc ctctttttgc ttttttatga tgggaaataa gtgttagtta
     1441 aagatgaata tactttttat agaaaggaaa agaatgtaat taagtggaaa aaatagtaat
     1501 tattggttca aaccattcag ggctagccgc agctcgattt ctaaacttaa gcgattcaaa
     1561 ttatgaagtt attctaatcg ataaaaaatc agaaattagt tacttggaat gtgggacatc
     1621 gttatttata aaaggagaag taagtaattt agaagatctt ttttatcaaa atagaatgac
     1681 actaaattct gagataaaaa agttatttct agagacagaa gttgacaaaa ttgat
//
LOCUS       DACDKM010000216         1675 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20834_2016
            SAMN12640370-rid9891663.denovo.220, whole genome shotgun sequence.
ACCESSION   DACDKM010000216 DACDKM010000000
VERSION     DACDKM010000216.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640370
            Sequence Read Archive: SRR10040891
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1675)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1675)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742835.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742835.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:04:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,012
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 29 of 75
            Pseudo Genes (incomplete)         :: 33 of 75
            Pseudo Genes (internal stop)      :: 19 of 75
            Pseudo Genes (multiple problems)  :: 5 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1675
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640370-rid9891663.denovo.220"
                     /isolate="E20834_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            193..807
                     /locus_tag="IV334_002828"
     CDS             193..807
                     /locus_tag="IV334_002828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001261742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAP8944828.1"
                     /translation="MRVAYIRVSSIDQNEQRQIEEMKKFGAERIFIEKQSGATITHRP
                     VFQEALDYVRDQDIFIVEAIDRLGRNYDEIIASVNYLKKKNVKLIITSLPIMAEAIGN
                     PLLDKFIKDLIIQILAMIAEQERTESKRRQAQGIKIAKANGVYKGRPKLYSADAKDPQ
                     RRLVYKSIVEDLKNGVAIAKIAKDYNVTRQTVYRIKKDSMVNDK"
     gene            complement(972..>1675)
                     /locus_tag="IV334_002829"
     CDS             complement(972..>1675)
                     /locus_tag="IV334_002829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000455809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8944829.1"
                     /translation="ELKTLPNSVNYIIRDITSEAQQLVNYMHGHESIPFSLILLKRSK
                     NISNTRDRFISIITACEIGVKEFYQQEKPDLTLIFENLQSPPVDKLLGSMFKGYFNIE
                     FPKEIRKEIKNFIEKRNKLVHSPQGSKPTIEECFECYMAVLKTFNFFYKINDSFMYRD
                     FYEESIELTPTEPNKSQITLSKELERKVESQELAIGISITNVSTYKELYEAKSQDNKD
                     FVENIDKEPTNTVGD"
BASE COUNT          565 a          276 c          260 g          574 t
ORIGIN      
        1 aatcttaaca gataaatcaa gttatatact agaaaattca taaaatatat ttttaatgtc
       61 ataatcgttt aaattttctt gattgacatt tatttacaaa aaatattgcc atttaaaaag
      121 aaaagtgaca tatggcttcc tcgtgtatga ttagggtgta ttttaaatag ttgcagaaag
      181 taggtaaaag ttttgagagt tgcatacata cgtgtctcat cgatcgatca aaatgaacaa
      241 cgccagattg aagaaatgaa aaaattcggt gcagagagaa ttttcatcga gaaacaatct
      301 ggcgcaacga ttactcatcg acctgttttc caagaagcat tagattacgt ccgagatcaa
      361 gatattttta ttgtggaagc tattgatcga cttggaagaa attatgacga gatcatcgct
      421 tcagtcaatt acttgaagaa gaaaaatgtg aagctgatca ttactagttt accgatcatg
      481 gctgaagcaa ttggcaatcc tctcctggat aaattcatta aggatctgat catccaaatt
      541 ttggccatga tcgctgaaca agaacggact gaatcgaaac gtcgccaggc acaaggaatt
      601 aagatcgcaa aagcaaacgg cgtctacaaa gggcggccca aattatacag tgcagacgca
      661 aaagatcccc aacgtcgcct tgtttacaaa agcatcgttg aagatcttaa aaatggtgtt
      721 gcgattgcta agattgctaa agactacaat gttacgagac aaaccgtgta tagaataaaa
      781 aaagacagca tggtcaatga caaatagttg atcatgctgc ttttattatc ttctcatctt
      841 taatttccta catataatta ctatctataa aattaagtct tactcagaac agataaagca
      901 taaaattcca acaaaccatt ttttacttaa ctaaataaag agtctgttaa tataatcttc
      961 acttatttac gttaatcccc tactgtattt gttggttcct tatctatatt ttctacaaaa
     1021 tccttattat cttgtgattt agcttcgtat aattctttgt atgtcgacac attggttata
     1081 ctaattccta tagctagttc ttgagactct acttttctct ctagctcctt tgacaaagtg
     1141 atttgagatt tatttggttc cgttggagtc aattctatag attcttcata aaaatctcta
     1201 tacataaagc tatcattaat tttataaaaa aaattaaatg tttttagcac tgccatataa
     1261 cattcaaaac attcttctat agttggttta cttccttgag gagaatgaac caatttatta
     1321 cgtttttcta tgaaattttt aatttccttt cttatttctt tcggaaactc aatattaaag
     1381 taccctttaa acatgctccc taataatttg tctacagggg gagattgtaa attctcaaat
     1441 attagagtta aatcaggttt ctcttgttga taaaattcct ttacccctat ttcacaggct
     1501 gtaattatcg aaataaacct atctctagta tttgatatat ttttacttct tttaaggagt
     1561 ataagagaaa aggggatact ttcgtgtcca tgcatgtaat taactaattg ttgagcttca
     1621 gaagttatat ctctaattat atagttaacg gaattaggaa gtgtctttaa ctcta
//
LOCUS       DACDKM010000217         1649 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20834_2016
            SAMN12640370-rid9891663.denovo.221, whole genome shotgun sequence.
ACCESSION   DACDKM010000217 DACDKM010000000
VERSION     DACDKM010000217.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640370
            Sequence Read Archive: SRR10040891
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1649)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1649)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742835.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742835.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:04:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,012
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 29 of 75
            Pseudo Genes (incomplete)         :: 33 of 75
            Pseudo Genes (internal stop)      :: 19 of 75
            Pseudo Genes (multiple problems)  :: 5 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1649
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640370-rid9891663.denovo.221"
                     /isolate="E20834_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(27..1508)
                     /locus_tag="IV334_002830"
     CDS             complement(27..1508)
                     /locus_tag="IV334_002830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M protein trans-acting positive regulator"
                     /protein_id="HAP8944830.1"
                     /translation="MLIEARKKELLVLQMVIQKNNCSLQELSNDLHIPKRTIKELIRK
                     INVSLKQFLLIDEFIYSNHKGEIQTKETDQTKKLAVFSKLKLCYLKESNRFNYLMLLI
                     NYPQTAVPKKYLLEQLYISPSYLEKLTKQLNHSLKKFHLRILSVKNCCTLEGNELSIR
                     LYLYFFLSDTFGGNDWPFSSLELNALKMNSTDEQIEKISKQEKNIYLLLTLFILRNKQ
                     QAYFPKTSSEITELMDLLQHTHDYSEYFTSFSVSRPCEETETERAYFNYFFYCFFPNQ
                     ISFRQKQEIGFLFSTSKNLICRQVGQLMTYLTQQFPLNHLENKIPIYFYFLVLAVVFF
                     ELLGESSSSFSTLCFPEATSNHSSSFIQEKKLREKIDTIFSKNGERNQISQYLSQLIQ
                     HLLQTETKEQLLIHLQITKQFTGNYHLQSHLQQLFRKDAVKITAYSEHADLIITDQHE
                     KISDETETFYLDTTHNTVLFGKLIKAVHELYMKKGYENKTKRM"
BASE COUNT          514 a          226 c          286 g          623 t
ORIGIN      
        1 aataagagga tgtgacaaga attttgtcac atcctctttg ttttgttctc ataacctttt
       61 ttcatataca gttcatgaac cgcttttatt aacttgccaa ataacactgt attgtgcgtt
      121 gtatccagat agaaagtctc cgtttcatct gaaattttct catgctgatc tgtaatgatc
      181 aaatcagcat gttctgaata tgcggtaatt ttaacagcat ctttgcgaaa taactgctgc
      241 aaatggcttt gaagatgata atttcctgta aactgtttgg ttatctgcaa atggatgagt
      301 aactgctctt tcgtttctgt ttgtagtaag tgttggatca attgactaag gtattgactg
      361 atttggtttc tttccccatt ttttgaaaaa attgtatcga ttttttctct taatttcttt
      421 tcctgtataa aagaactaga atgattggac gtggcttctg gaaagcaaag tgtagaaaaa
      481 ctactgctgg attcacccaa cagttcgaaa aaaacaactg ccaacacaag aaaataaaaa
      541 taaattggta ttttattttc taaatggttg agaggaaact gttgagttaa gtaagtcatc
      601 aattgaccga cttgtcgaca aataaggttt ttgcttgtgg aaaataaaaa accaatctcc
      661 tgtttttgtc gaaaagagat ttgatttgga aaaaaacaat agaaaaaata attaaagtag
      721 gctctctctg tttctgtctc ttcacaaggg cgactcactg aaaaagaagt aaagtattct
      781 gaataatcat gtgtatgctg caataagtcc atcaattcag tgatttcaga agaagttttt
      841 ggaaaatatg cctgttgttt gtttcgtaat atgaataaag ttaacagcaa gtagatattt
      901 ttttcttgtt ttgaaatttt ttctatttgc tcatctgtac tgttcatttt tagtgcattt
      961 aattcaagtg aggaaaatgg ccaatcgtta cctccaaatg tatctgaaag aaaaaagtaa
     1021 agatagagac ggatagataa ctcatttcct tcaagagtac agcaattttt tacagatagg
     1081 atccgtaaat ggaatttttt taaggaatga ttgagctgtt ttgtcagttt ttcgagatag
     1141 gagggcgata tataaagttg ttcaagtaaa tatttttttg gtacagcagt ttgtggataa
     1201 ttgattagta acatcaaata gttaaaacga ttggattctt ttaggtagca taattttaac
     1261 ttagaaaaaa cagctaactt tttagtttga tcggtttctt tggtttggat ctctccttta
     1321 tgattagaat aaataaattc atcaatcaat aaaaattgct tgagtgatac atttattttt
     1381 cggatcagtt ctttgattgt ccgttttgga atatgtaagt cattggataa ttcttgtaaa
     1441 gaacaattat ttttttgaat gaccatttgt agaacaagta actctttttt tcttgcttca
     1501 attagcataa atacctcctt tttattctgt ttttgtatat agttagacaa aaactgtgag
     1561 tggaaagtca tttcagtctg tgttattctc acagagtgtc gccttctttt ttatataacg
     1621 ttatttaatt tttttgttac cttgtttat
//
LOCUS       DACDKM010000218         1626 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20834_2016
            SAMN12640370-rid9891663.denovo.223, whole genome shotgun sequence.
ACCESSION   DACDKM010000218 DACDKM010000000
VERSION     DACDKM010000218.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640370
            Sequence Read Archive: SRR10040891
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1626)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1626)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742835.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742835.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:04:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,012
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 29 of 75
            Pseudo Genes (incomplete)         :: 33 of 75
            Pseudo Genes (internal stop)      :: 19 of 75
            Pseudo Genes (multiple problems)  :: 5 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1626
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640370-rid9891663.denovo.223"
                     /isolate="E20834_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            136..1164
                     /locus_tag="IV334_002831"
     CDS             136..1164
                     /locus_tag="IV334_002831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HAP8944831.1"
                     /translation="MNSKKLQMGEIVRGPSQISIDVTYKCNFRCKHCYNASGGDNCFM
                     DNELTDDELLELTKDICNLKPYNICFCGGETLLRKKIVLSCATLLKEAGIPNIAIVTN
                     GSLVTQDVAKELFKAGINRFQVSLDGKNASSHEALRLYPNSFEKAINAIKLFRELSEE
                     VDVAFCPTSFNYNQLREVYQLVNTLKVKSLRIQPFMLSGRGMQNREIVPTVSQYRKLV
                     SDINYIKRNEDYTKIDWGDPIDHIIRHRTLLKDFSNFVSIRSDGGLMASPYLPIQVGN
                     IKCHSLSKYWRAGLYKVFSLDLVQDIATQVSSIQDMSKELKKGHIKWLNEDIEIDILD
                     EYKHDILI"
     gene            1220..1438
                     /locus_tag="IV334_002832"
     CDS             1220..1438
                     /locus_tag="IV334_002832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8944832.1"
                     /translation="MISEPQMNNMYESPTIMGAGEAAPRACSAIMVCVLLASAGVGIQ
                     AGAVYTYAVVATVAVAAAAVWKTVGLVG"
BASE COUNT          610 a          186 c          307 g          523 t
ORIGIN      
        1 gcgataattt gagggattat atactaaata caaaaaaaga agtttgttat ataattataa
       61 ctcatgatca cattctagat aattttttgg attcaaatga tgaaatatta tatttaaata
      121 aggagtatga taataatgaa tagcaaaaaa cttcagatgg gtgaaattgt tagaggtcct
      181 agtcaaatat ctattgatgt tacatataaa tgtaatttta gatgtaaaca ttgttacaat
      241 gcgagtggtg gagacaattg ttttatggat aatgagttga ctgatgatga actgttagaa
      301 cttacaaaag atatatgtaa tttaaagcca tataatattt gtttctgtgg aggagaaact
      361 ttactgagaa aaaaaatagt tttatcttgt gccactttat taaaagaggc aggtattcct
      421 aatattgcta ttgttactaa tggatcatta gtaactcagg atgttgcaaa agaattattc
      481 aaagcaggta ttaatcgatt tcaagtaagt ttagatggaa aaaatgctag tagccatgaa
      541 gcacttagat tatatccaaa cagttttgaa aaggcaatta atgctatcaa attatttaga
      601 gaattgtccg aagaagtgga tgttgctttt tgtcctacta gttttaatta caatcaatta
      661 cgagaagtat atcaactagt aaatactctt aaagtgaagt ctttaagaat tcaaccattt
      721 atgcttagtg gtaggggaat gcaaaatagg gaaatagtgc caactgtttc tcaatataga
      781 aaactagttt ctgatattaa ctatattaaa agaaatgaag attataccaa gatagattgg
      841 ggagatccaa ttgatcatat catccgacac agaactttat tgaaagattt ttcgaatttt
      901 gtttctatta ggtcagacgg aggattaatg gcatcacctt acttgccaat tcaagttgga
      961 aatattaaat gtcacagtct ttctaaatat tggcgagcag gactttataa agttttttct
     1021 ttagatttag ttcaagatat agctactcaa gtttcttcaa tacaagatat gagtaaagaa
     1081 ttaaaaaaag gacatattaa atggcttaat gaagatatag aaattgatat tctcgatgaa
     1141 tacaaacatg atattttaat ttaggtttat cattggaaag tttagataaa caaagtactt
     1201 ttagaaagga gggaaaaaaa tgatttctga acctcaaatg aataatatgt atgaaagccc
     1261 aactataatg ggagctggtg aagctgcgcc aagagcttgt tcagcaatta tggtgtgtgt
     1321 gttattagca tctgcaggag taggtatcca agcaggagcg gtgtatactt atgctgtagt
     1381 tgctacagta gctgtagctg cagccgcagt ttggaaaaca gtaggattag ttggttagga
     1441 attaatagga gactaactgt ctcctattga ttccaatgat taagctaagg aggaatttta
     1501 agaaaataat gaaatacaat aatttaaaac cgaaattact taatgagaac atagaatata
     1561 taagagaaga aaattatgga tatttaacca tagttcccaa aaatggaact gaattgaaag
     1621 ataacg
//
LOCUS       DACDKM010000219         1592 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20834_2016
            SAMN12640370-rid9891663.denovo.224, whole genome shotgun sequence.
ACCESSION   DACDKM010000219 DACDKM010000000
VERSION     DACDKM010000219.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640370
            Sequence Read Archive: SRR10040891
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1592)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1592)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742835.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742835.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:04:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,012
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 29 of 75
            Pseudo Genes (incomplete)         :: 33 of 75
            Pseudo Genes (internal stop)      :: 19 of 75
            Pseudo Genes (multiple problems)  :: 5 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1592
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640370-rid9891663.denovo.224"
                     /isolate="E20834_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..691
                     /locus_tag="IV334_002833"
     CDS             <1..691
                     /locus_tag="IV334_002833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HAP8944833.1"
                     /translation="ITIRQREQYEQQLKRKELVYLKKYTDSLEKDQEKFARFRHDYKN
                     LLLSLKEIANQNHDSILEKEIKELEIYSNSYLTNKFEYRYLKNIKNEYLKSLLIAKLY
                     QANNNKICCKFECPSTIIEVPVPIFDCIRVLGIILDNAIEASIESQDRELSLAIYRDD
                     KQIEISISNSCREILLSIDTLMKKGITTKKGHQGLGLSSIEEINKKNPNMFVNYQNDP
                     YQFTTQVILLF"
     gene            706..1458
                     /locus_tag="IV334_002834"
     CDS             706..1458
                     /locus_tag="IV334_002834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP8944834.1"
                     /translation="MSYPIILCEDQIIQLNQLERIIDNFILFHDKVFKIVLKTQSPLE
                     VKKYLKQFRPKQGIYFLDIDLNHEVNGIELAEVIRKYDVQAKIIFTTTHDEMLPVTIK
                     RRVETLGFVTKDQTLDEYRNEIVELLLLAQERIDATKQVTNQAFVFSIGSQTFTFDIN
                     DIYFVEASKLPHRLSLCTKDGQYEFYGRISELEKKYPMLTRISRACLVNIFNVKEIDF
                     KKRSLYFDSELARNFTLGKAQKIKEKLKESTV"
BASE COUNT          632 a          192 c          266 g          502 t
ORIGIN      
        1 aataactatt aggcaacgag aacaatatga acagcaattg aaaagaaaag aattggttta
       61 tctaaaaaaa tatactgatt cactggaaaa agatcaagaa aaatttgcca gatttagaca
      121 tgactataaa aatttattgc tgagtttaaa agaaatcgct aatcaaaacc atgattcgat
      181 attggaaaaa gaaataaaag aattagagat ctattctaat tcttatctaa ccaataagtt
      241 tgaatatcgt tatttaaaaa atataaaaaa tgaatatttg aaaagtttgt tgatagcgaa
      301 gctataccaa gcaaataaca ataagatttg ttgtaaattc gaatgtccaa gtactattat
      361 tgaagttcct gttccaatat ttgactgtat tagggtgctt ggaattattc tcgataatgc
      421 aattgaagca tctatagaga gtcaagatag agaactgtct ctagctattt accgagatga
      481 taaacagata gaaatttcga tatcgaatag ttgtagagag atactactct ctatagatac
      541 tctaatgaaa aaaggaataa caacaaaaaa gggacatcaa gggttaggtt tatctagtat
      601 agaggaaatc aataaaaaga atcccaatat gtttgtgaac tatcaaaacg atccgtatca
      661 atttactact caagttattt tgttgttttg agggaaggag gatttatgag ttatccaata
      721 atattatgtg aagatcaaat tatacaatta aatcagttag aacgaattat tgataatttc
      781 attctttttc acgataaagt atttaagata gtattgaaaa cacaaagtcc gttagaagtt
      841 aaaaaatacc tcaaacaatt ccgaccaaag caaggaatat atttcttaga tattgattta
      901 aatcatgaag ttaacggtat agaattagca gaagtaatca gaaaatatga tgttcaagca
      961 aaaatcattt ttacaactac tcatgatgag atgttacccg taacaataaa aagaagagtt
     1021 gaaacgttag gatttgtaac aaaagatcaa acactagatg agtatcgaaa cgagattgtt
     1081 gagttattgt tattagcgca agaaaggata gatgcaacaa aacaggttac gaatcaagca
     1141 tttgtttttt ccataggttc tcaaacattt acttttgata taaatgacat ttattttgta
     1201 gaagcttcaa aattaccaca tagattatca ttgtgtacta aagatgggca gtacgaattt
     1261 tatggaagaa ttagtgagct tgaaaagaaa tatcctatgt taacaagaat tagtcgagcg
     1321 tgtttagtaa atatctttaa cgttaaagaa atagatttta aaaaacgttc actatatttt
     1381 gattcagagt tggccagaaa ctttacttta gggaaagcac aaaaaattaa ggagaagttg
     1441 aaagaaagca ctgtttagtg gttttttctt tagggagatt gcatttttct aataaaatag
     1501 attccttcac tagtgcaaat ttttctgaat cctattaaag ttgtaccaat ttttatagct
     1561 gttattagtt acgtgcataa cagcaatatt ag
//
LOCUS       DACDKM010000220         1558 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20834_2016
            SAMN12640370-rid9891663.denovo.225, whole genome shotgun sequence.
ACCESSION   DACDKM010000220 DACDKM010000000
VERSION     DACDKM010000220.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640370
            Sequence Read Archive: SRR10040891
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1558)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1558)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742835.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742835.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:04:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,012
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 29 of 75
            Pseudo Genes (incomplete)         :: 33 of 75
            Pseudo Genes (internal stop)      :: 19 of 75
            Pseudo Genes (multiple problems)  :: 5 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1558
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640370-rid9891663.denovo.225"
                     /isolate="E20834_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            314..1444
                     /locus_tag="IV334_002835"
     CDS             314..1444
                     /locus_tag="IV334_002835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HAP8944835.1"
                     /translation="MGPYLTLSMIGLLEVYSASSYRLLQADENTKSLLLRQLIFIFLS
                     WSVIFLARSVKLHYLLHPKIAGYGLALSIFFLVLVRIGIFGVTVNGAQRWISLFGIQF
                     QPSELANLFLIFYLSWFFRDGNSSPKDLKKPFLITVGITFLILFQPKIAGALMILSIA
                     WVIFWAAAVPFKKGIYLIVTFSALLIGAAGGVLYLGNKGWLPQMFNHAYERIATLRDS
                     FIDSHGAGYQMTHSFYALYNGGIFGRGLGNSITKKGYLPETETDFIFSIITEELGLIG
                     ALCVLFLLFSLCMRIFCLSSRCKNQQAGLFLLGFGTLLFVQTIMNVGSIAGLMPMTGV
                     PLPFVSYGGTSYLILSLGIGITLNISSKIQAEELALYRPEKQ"
BASE COUNT          437 a          257 c          339 g          525 t
ORIGIN      
        1 taaccttgtt aggtgaggct cctatacaaa cataggctgt tgcccagaaa cgtcgaaagt
       61 cgccaatggg taaaacagaa actgttggat taaggctttt tccaatagct aagaatgttt
      121 tctttacgtt gtatagtgct aaaactcgga cgatgagatg atgctaggtc agaattcttg
      181 ttcgaaaagg tttctgacct tttctttata tttttgtgac tgacttattt gatgagacaa
      241 actcgctcct cacctgacaa gtggattgct gtgtgaggag gagatgcggt gaaaaaagat
      301 cgattggtgg attttgggtc cttatttgac tttgtccatg atcggtcttt tagaagtata
      361 cagtgccagt tcttatcgtt tattgcaagc cgatgaaaat acaaaaagtc tattattgcg
      421 tcaactaatt ttcatatttt tgagttggag cgtgatcttc ttagctcgtt cagtcaaact
      481 acactattta cttcacccta aaatagcagg atacggttta gccttatcga ttttcttttt
      541 agtattagta agaataggga tattcggtgt cactgtcaac ggcgcacaac gttggatctc
      601 tctgtttggc attcaattcc agccttctga actggcaaat ctttttttga ttttttattt
      661 aagctggttt tttcgtgacg gaaatagtag cccaaaagat ctaaaaaaac cattcctgat
      721 tacagtaggt ataacttttc tgattttatt tcagccaaag attgctggag cattgatgat
      781 cctttcgatt gcgtgggtca tattttgggc agcggcggtt ccatttaaaa aagggatcta
      841 tctaatcgtt actttttctg cattgctgat tggagcagca ggcggggtat tatatttagg
      901 aaataaaggt tggcttccac agatgtttaa tcatgcttat gaaaggatcg cgactttaag
      961 agattctttt atcgatagtc atggtgcggg ctatcaaatg acccattcgt tttatgcact
     1021 atataatggt ggcatatttg ggcggggatt aggaaatagt atcacaaaaa aagggtatct
     1081 tccagagaca gaaacagatt ttattttttc gattattaca gaagagcttg ggctaatcgg
     1141 tgcattgtgt gtgcttttct tattgttctc attatgcatg agaatctttt gtttaagcag
     1201 tcgctgtaaa aatcagcaag ctggattgtt tcttttagga ttcggaactt tgctattcgt
     1261 tcagacgata atgaacgtgg gaagtatcgc ggggctgatg ccaatgactg gtgtgccatt
     1321 gccatttgtt agctacggag gaacaagtta tctgatcctt tctttaggaa tcggaatcac
     1381 gttgaatatt tcttcaaaga tacaggccga agagttggcg ctctatcgac ctgaaaaaca
     1441 ataaagagaa actgagaaac tctcagtttg aattatcata aagtacaatc ttttattttt
     1501 caagcgagag gttcttttag aaagaggaat tcgcaatgaa agtatctcaa aaaatcat
//
LOCUS       DACDKM010000221         1521 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20834_2016
            SAMN12640370-rid9891663.denovo.226, whole genome shotgun sequence.
ACCESSION   DACDKM010000221 DACDKM010000000
VERSION     DACDKM010000221.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640370
            Sequence Read Archive: SRR10040891
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1521)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1521)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742835.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742835.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:04:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,012
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 29 of 75
            Pseudo Genes (incomplete)         :: 33 of 75
            Pseudo Genes (internal stop)      :: 19 of 75
            Pseudo Genes (multiple problems)  :: 5 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1521
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640370-rid9891663.denovo.226"
                     /isolate="E20834_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(157..555)
                     /locus_tag="IV334_002836"
     CDS             complement(157..555)
                     /locus_tag="IV334_002836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system death-on-curing
                     family toxin"
                     /protein_id="HAP8944836.1"
                     /translation="MRYLSTKEIIKINAKVILRHSPGEMIGVKDANALDMAVKQPSQA
                     VFNQELYPEIYEKAAILAINLAKKHPFHNGNKRTALVAMLLFLQLNNCPVAFSRQEAV
                     DFIIMITTSSLDFDSLKSKITSYLRQTAIK"
     gene            complement(552..788)
                     /locus_tag="IV334_002837"
     CDS             complement(552..788)
                     /locus_tag="IV334_002837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HAP8944837.1"
                     /translation="MKKMKKYKIRKSGNSDVTTIPPEVKEFMGVQTGDAISYVFQSDG
                     SVRMIKAQEEPDIDSLVDSIMNQYEDALKDLVDL"
     gene            complement(948..1265)
                     /locus_tag="IV334_002838"
     CDS             complement(948..1265)
                     /locus_tag="IV334_002838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8944838.1"
                     /translation="MKKVHSFLNYLMGAITIVAVYQAFLKYPDYGTKLLSLTHNQTAY
                     SPTLILSTLTAGAKSATYFFHKTTSMLKRKKQRKQLLVVVAAMIIIYFLPVITQVFHQ
                     YFG"
BASE COUNT          467 a          270 c          211 g          573 t
ORIGIN      
        1 cactcttttg tattcctttt aaatcaatta tttcaaaatt aatccatatt ttccatattt
       61 ttatgaaaaa actattttgt atgattaaac ataaaaaaat cagcatattc cttaatgaca
      121 tgctgatcta attgagaaat aaagttttta attcgttcat tttattgcgg tttgtcgtaa
      181 ataactggtt atcttggact ttagtgaatc gaaatccaat gaactagtgg taatcattat
      241 gataaaatca acagcttctt gacgagaaaa agcaactgga caattattca attgaagaaa
      301 caacaacatg gcaacaagag cagttcgttt attgccatta tgaaaaggat gttttttagc
      361 taaattaatt gctaaaatcg ccgctttttc atagatttca ggatagagtt cttgattgaa
      421 tactgcttgt gaaggctgtt tgacggccat atctaatgca tttgcatctt ttacaccgat
      481 catttcgcct ggcgagtgac gtaaaataac tttcgcgttg attttgataa tttctttagt
      541 cgaaagatac ctcataaatc aaccaaatct tttaacgcgt cttcatattg attcatgatc
      601 gaatcaacta atgaatcaat atcaggttct tcttgtgctt tgatcattcg tacagaacca
      661 tcagattgaa aaacataact gattgcgtca cctgtctgca cacccatgaa ttcttttact
      721 tctggtggga tagttgttac atcgctattg ccagattttc taattttata ctttttcatt
      781 tttttcatat ttcgccaccc tctataccat tctcattcta tagtaagtac attatatcca
      841 agtatatact tttaatcaat gtatatactt ttattttgct ttttcttacg aaaaaatgac
      901 aaagaaaaaa gtttgtgaca tgacttttgt cacaaactct cgtattctta accaaaatat
      961 tgatgaaata cttgtgtaat aactggtaga aaataaataa tgatcatagc cgctaccacc
     1021 accaacaatt gttttcgctg tttctttcgc tttagcatgc ttgtcgtttt atgaaaaaaa
     1081 taagtagcgc ttttagctcc tgcagtcaaa gtcgataaaa tcaacgttgg tgaatatgct
     1141 gtttgattgt gcgtcaatga caacaacttc gtcccgtaat caggatactt tagaaatgct
     1201 tggtacacag ccacaatcgt gattgctccc ataagataat ttaaaaatga atgcactttt
     1261 ttcaaataaa aaaactcctt gctatctata taaaatcagc ctaccccaac gtttttgctt
     1321 gattcactga tattttttag ttttattggt atttatattc tcacttttcg gaataaatat
     1381 tccgctattt cattgttttc atttactcta cttcgttttc attatttttt cttgatgttt
     1441 ctttgtttcc atcaactgct tttgttccac tgttttcgat ccatggaaaa gaaaaaccga
     1501 ttgtcatatc ttcttttaat a
//
LOCUS       DACDKM010000222         1498 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20834_2016
            SAMN12640370-rid9891663.denovo.227, whole genome shotgun sequence.
ACCESSION   DACDKM010000222 DACDKM010000000
VERSION     DACDKM010000222.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640370
            Sequence Read Archive: SRR10040891
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1498)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1498)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742835.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742835.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:04:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,012
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 29 of 75
            Pseudo Genes (incomplete)         :: 33 of 75
            Pseudo Genes (internal stop)      :: 19 of 75
            Pseudo Genes (multiple problems)  :: 5 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1498
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640370-rid9891663.denovo.227"
                     /isolate="E20834_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            316..570
                     /locus_tag="IV334_002839"
     CDS             316..570
                     /locus_tag="IV334_002839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HAP8944839.1"
                     /translation="MGKELKVRKIGNSVGVILPSSLGLKSGDIIQAKQEDNLFILDTT
                     QIAKEHDRKLIEESFQDFEKGLTVSEIEMVKAFGKYGWSE"
     gene            571..888
                     /locus_tag="IV334_002840"
     CDS             571..888
                     /locus_tag="IV334_002840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HAP8944840.1"
                     /translation="MKEYTLVYSKSFQTSLETIIEEWENELALSPASIKRFVGAIQKA
                     LELTKNFPTMHEEVSEIYGMDIPTYRIVIGKSYALFYRIDHEKKEILVGRIFQSKQMK
                     LGF"
BASE COUNT          531 a          228 c          290 g          449 t
ORIGIN      
        1 acaaaataaa aaggcaatac gccttagttg tcggtcttcg agttaccaac ggtaaccctc
       61 ataccgaacg gcattcatca cccacctata gaggatgggc gacttctgcc taattctgtt
      121 aaatggatgt taacggaagt gtatggtgag tagttcactc gtattttgat atcgtttgta
      181 tatgttttca gcgttctaag tggcttattt tgtccaataa taatatggag tggaatccct
      241 taagaaacac cgttggcaat tgttgttata acatgttata atttattcat caaaaactaa
      301 aaggagtgtg ataacatggg aaaagaacta aaggttagaa agatcggcaa tagcgtggga
      361 gtgatccttc caagctctct tggattaaaa agcggagaca ttatccaagc caaacaagaa
      421 gacaacttat tcatattaga tactactcaa atagcaaaag aacacgatcg caaattgatt
      481 gaagaaagtt tccaagactt tgagaaaggt ttaacagttt cagaaattga aatggtaaaa
      541 gctttcggaa aatacggctg gagtgaatag atgaaagagt acacgctagt ttattctaaa
      601 agttttcaga ccagccttga aacgataata gaagaatggg aaaatgagct tgctctttct
      661 ccagccagca taaaaagatt tgtaggtgcg atccagaagg ctttagaact aaccaaaaat
      721 tttccaacta tgcatgagga agtttccgaa atctatggaa tggatatccc aacatatcgg
      781 atcgtgatcg gcaaaagcta tgccttattt tatcggatcg accatgaaaa aaaagagata
      841 cttgtaggac gtattttcca atctaaacaa atgaaactag gcttttaaca ataattagga
      901 aaaagtcgct catcctctac aggtgggtga ttaatgccgc tcgatatggt tgttaaccgt
      961 cggttactcg aagattgata tataagactt agtacctttt tattttgctc catgttatgg
     1021 ccgaaggtct aaagatacaa tcattagtct agagaaaaat taagaacctt aggaactacg
     1081 gggatcgctt ggtgaataag agacactgct attagtgaaa aaataagata tcaagtacgc
     1141 tctattccca aaaatttcgt gactttactc atgagaggtt caagtagtat atttttaatc
     1201 aaaattgacg taacttttaa taaatactac ctaaataagg tagtatttat ttttgtgttt
     1261 taaaagagga agtccaatat atctatggga aacttcttaa atattatgtt ggtacatgaa
     1321 aatatacttc cctgctagac tatttacaac attttacttt gtacgtattc ttgttttaaa
     1381 aggaatggga taaaatatag gtacaataac tatttgatgc taaatataat taatggagga
     1441 gagatagtga acatacttaa tgaaaaaaga tatgtatggt ttgctggtaa aaaatata
//
LOCUS       DACDKM010000223         1489 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20834_2016
            SAMN12640370-rid9891663.denovo.228, whole genome shotgun sequence.
ACCESSION   DACDKM010000223 DACDKM010000000
VERSION     DACDKM010000223.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640370
            Sequence Read Archive: SRR10040891
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1489)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1489)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742835.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742835.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:04:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,012
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 29 of 75
            Pseudo Genes (incomplete)         :: 33 of 75
            Pseudo Genes (internal stop)      :: 19 of 75
            Pseudo Genes (multiple problems)  :: 5 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1489
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640370-rid9891663.denovo.228"
                     /isolate="E20834_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            4..603
                     /locus_tag="IV334_002841"
     CDS             4..603
                     /locus_tag="IV334_002841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAP8944841.1"
                     /translation="MNGFEKRAQEKKKEILQAAFDLMNTDIGTAGVTMDKVAENAQVS
                     KATIFKYFGSKEGLIYEVFFAFMNDLGQEAKALIAENLPFEETLIAMSENKIRYLNRV
                     NKQFYIDLMTYYTKKEKKEFSDAMDAYTQESFSIMLDLFHRGRKEGKVDLKYSDEFLI
                     LYFQAMVEGISKPEIYAKLLPYTAEWTEVLIKGIAPKKK"
     gene            complement(672..>1489)
                     /locus_tag="IV334_002842"
     CDS             complement(672..>1489)
                     /locus_tag="IV334_002842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HAP8944842.1"
                     /translation="NDLIQKDEQGNLSFNGLALPEGKKPLVLSQDDVSYYEYMDNSGF
                     PSRLVVSNNNEIKNVYKEKDKEEIGDFDMVPLIDAFVKKHPDFSYRGAKGTLALTGYN
                     GVLGYRTSKSQYGDNEKTKKEIKEAKKVAEQLKKDGWSFASHTWGHLNMTQASLEDVK
                     KDNELWQQEVAPILGKTNILIYPFGADISDWQPYSAENQKFAYLKSQGFDIFCNVDAS
                     TPAWGQLGTDFYRNARINIDGIRFQSDLDGKNPILDPFINVHDVYDKEARESA"
BASE COUNT          477 a          263 c          296 g          453 t
ORIGIN      
        1 caaatgaacg gttttgaaaa gagagcacaa gagaaaaaga aagagatttt acaagcagct
       61 tttgacttga tgaacacaga cataggaacc gctggagtga caatggataa ggtagctgag
      121 aacgctcagg taagtaaagc gacgattttt aaatattttg gcagcaagga agggctgatt
      181 tatgaagtat tttttgcatt tatgaatgat ttagggcaag aagcgaaagc gttgattgcg
      241 gaaaatcttc catttgaaga aacactgatt gccatgagtg aaaacaaaat ccgttatctc
      301 aatcgagtga acaaacaatt ttatatcgat ttgatgacat actatacaaa aaaagaaaaa
      361 aaagaatttt ctgacgcgat ggatgcgtat acgcaagaaa gtttcagtat catgcttgat
      421 cttttccatc gagggcggaa agaaggaaaa gtggatttga aatattcgga tgagttcttg
      481 atcctctatt ttcaagcaat ggtagaagga atttctaagc cggagatata cgcaaaactg
      541 cttccatata cagcagaatg gacagaagtc ctgatcaagg gaatcgcacc gaagaagaaa
      601 taaagcataa aaaagccgaa actgacgata aaatgtcagt tcggcttttt tggttcataa
      661 atgacgtaaa atcaagcaga ttcgcgtgct tctttgtcat agacatcatg gacattgatg
      721 aatggatcaa gaataggatt ttttccgtct aaatcagatt gaaaacgaat gccatcgata
      781 ttgatccgag catttcgata gaaatccgta cctaattgtc cccaagctgg tgtagaagca
      841 tcgacattac agaaaatatc gaaaccttga ctcttcaagt aagcaaattt ttgattttct
      901 gctgaataag gctgccagtc gctgatgtct gcaccaaatg gataaatcag aatatttgtt
      961 tttcctagga taggtgcaac ttcttgctgc cacaattcat tgtctttttt tacatcttct
     1021 agtgaagctt gagtcatatt caagtgaccc catgtatggg aagcaaagct ccagccgtct
     1081 ttcttcaact gttccgccac ttttttagct tctttaatct ctttttttgt tttttcattg
     1141 tctccatatt gagattttga tgttcgataa cctaaaactc cgttgtaacc tgtcagggcc
     1201 aaggtacctt ttgcgcctcg ataagaaaaa tcaggatgtt tcttgacaaa ggcatcgatc
     1261 agaggaacca tgtcaaagtc accgatttct tctttatctt tctctttata gacatttttg
     1321 atttcattgt tatttgatac gaccaatcgg ctaggaaaac cggaattatc catatattca
     1381 taatagctga catcatcttg tgaaagaacc aatggttttt ttccttcagg tagcgcaagc
     1441 ccgttaaagg aaagatttcc ttgttcgtct ttttggatca aatcattta
//
LOCUS       DACDKM010000224         1485 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20834_2016
            SAMN12640370-rid9891663.denovo.229, whole genome shotgun sequence.
ACCESSION   DACDKM010000224 DACDKM010000000
VERSION     DACDKM010000224.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640370
            Sequence Read Archive: SRR10040891
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1485)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1485)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742835.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742835.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:04:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,012
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 29 of 75
            Pseudo Genes (incomplete)         :: 33 of 75
            Pseudo Genes (internal stop)      :: 19 of 75
            Pseudo Genes (multiple problems)  :: 5 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1485
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640370-rid9891663.denovo.229"
                     /isolate="E20834_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            287..694
                     /locus_tag="IV334_002843"
     CDS             287..694
                     /locus_tag="IV334_002843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8944843.1"
                     /translation="MSYRVQLIISEDVESQQFGTNVINKVINPALSINAPLIPTALSF
                     SVTAVVSEIEDTEKIKIVEIEVLNKNEKQIFSTGEVSVSLPPQVNDINFNINARNVLV
                     EEAGEHYAVVKFNGTEIGRQIFDIKVNKPVEKN"
     gene            708..1109
                     /locus_tag="IV334_002844"
     CDS             708..1109
                     /locus_tag="IV334_002844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8944844.1"
                     /translation="MLDIVPSNTHRSGSLINFAAIFACASVILASPVVVDASSTPKTK
                     NDNQLVITTKNEINTISSNDGKIFDVINTVLKRKAEINIKYDEDLNLYFFVIKTTSEL
                     FSSDYDVLDTLDNVLSDYKYMGKSVVATLGE"
     gene            1111..1482
                     /locus_tag="IV334_002845"
     CDS             1111..1482
                     /locus_tag="IV334_002845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8944845.1"
                     /translation="MFDFNEYLSISKQIESDDKYNSKESYRRTAVSRAYYSAFKKSDE
                     YLKENYDIIYNGSSGKGSHQTVWNLFSTVKELNTLGIHNSGYRMLEKRKCADYIPNET
                     ITKTDMALMNREAEKIINKLT"
BASE COUNT          571 a          183 c          258 g          473 t
ORIGIN      
        1 tgaggggaag tacatatagt tgttgatata gttgctaata ttgttagcta atttgttagt
       61 aaaactgtat acgaatttag atagtaaaaa aacagtttgt tccttatata gaggcacttt
      121 tactactgat taaatgtcag atatgcttac aaatatactt ataaatatgc ttacaaaatt
      181 agctaatatt tccgttgaca aagctttgtt actattgtta aactaaattg actagcaaaa
      241 gtgaaacata aatttattaa ataaatgtaa aaattaggag aaatcaatgt cttatagagt
      301 acaattaata atttcagaag atgtagaaag tcagcaattt ggcactaatg ttattaataa
      361 agtaattaat ccagctcttt ctattaatgc gcctttaatt cctactgctt tatcattttc
      421 tgtaacagct gtggtgtctg agatagagga tacagaaaag ataaaaatag tagaaattga
      481 agttttaaac aagaatgaaa aacaaatttt ttctacgggt gaagtatcgg taagcttgcc
      541 tcctcaagtt aacgatataa actttaatat aaacgccaga aatgtcttgg ttgaagaagc
      601 aggagagcat tatgctgttg ttaaatttaa tggaactgag attggaaggc aaatctttga
      661 tatcaaggtc aacaaaccag tggaaaaaaa ttaaggagac gatacggatg cttgatatag
      721 taccatcaaa tacacatcgg agtggtagct taataaattt tgctgcgatt tttgcttgcg
      781 catcggtaat tctagcatct ccagttgttg tggatgcaag ttctactccg aaaactaaaa
      841 atgataatca acttgtaatt actacaaaaa atgagattaa cacaatttca agtaatgatg
      901 gcaaaatttt tgatgtgata aatacggttt taaaaagaaa agctgaaatc aatataaaat
      961 atgatgaaga tttaaacttg tatttctttg ttatcaaaac aacatctgaa ttgttttcct
     1021 cagattacga tgttttggat actctcgata atgtattatc tgactataaa tatatgggaa
     1081 aatctgtagt agcaactttg ggggaataaa atgtttgact ttaatgaata tttatctatt
     1141 tccaaacaaa ttgaatcaga tgataaatat aattctaaag aatcttatag aagaactgct
     1201 gtttcaagag catactacag tgcttttaaa aaatctgatg agtatttgaa agaaaattat
     1261 gatattattt ataatggaag tagcggaaaa ggaagccacc aaaccgtttg gaatttgttt
     1321 tcaacagtta aggaattgaa taccttagga atacataata gtgggtatag aatgttagag
     1381 aaaagaaaat gtgctgacta tattcctaat gaaaccatta cgaaaacaga tatggcgctg
     1441 atgaatcgag aagcagaaaa aataataaac aaactcacct aatga
//
LOCUS       DACDKM010000225         1458 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20834_2016
            SAMN12640370-rid9891663.denovo.230, whole genome shotgun sequence.
ACCESSION   DACDKM010000225 DACDKM010000000
VERSION     DACDKM010000225.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640370
            Sequence Read Archive: SRR10040891
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1458)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1458)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742835.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742835.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:04:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,012
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 29 of 75
            Pseudo Genes (incomplete)         :: 33 of 75
            Pseudo Genes (internal stop)      :: 19 of 75
            Pseudo Genes (multiple problems)  :: 5 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1458
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640370-rid9891663.denovo.230"
                     /isolate="E20834_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..328
                     /locus_tag="IV334_002846"
     CDS             <1..328
                     /locus_tag="IV334_002846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8944846.1"
                     /translation="NRYGEKNILNSSLPFEVLLLENDFSSTLLISPISTISFSPSMMF
                     EKTINSIMLAKLILNEYTALSEDKKETLTNIVLFCEHYNRLSEEKILLPENWAELRKL
                     VEYGII"
     gene            315..>1458
                     /locus_tag="IV334_002847"
     CDS             315..>1458
                     /locus_tag="IV334_002847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HAP8944847.1"
                     /translation="MGLFNTLSKSKLVKSSFWYTIGNFFIKGISFITIPIFVNITTQA
                     EYGLINNFTSFVSIFSILVGLSLNSAINNASFDYKDDINEFMSSVLSLATLSLIVFLL
                     IGNIYFLFSNAFFDMNQLVFNLMLLQSFASFVISYIQSYFTINFNHFKYLILSFISTF
                     LNIAISVALMQTLYFDDKYVGRVVGGTLSFVLIGLVIYLRIMIKGAHFFDKKQWRYAL
                     KISLPLIPHSLANIVLAQFDRIMINSYLGPAAGGIYSYISNIGIILSVIWMSTNNAWV
                     PWFYGEMSKNNEKKVKKVSNYYLVVFTLITMIVMVVSIDLAKFMAPKDYYSGLSLVVP
                     ITLGYFFQFLYSLPVNAEFYEKKTSFIAIGTVASAIVNILLNMIFIP"
BASE COUNT          465 a          192 c          231 g          570 t
ORIGIN      
        1 aaatcgttat ggtgaaaaaa acatattgaa ttccagttta ccgtttgaag tcttgctact
       61 tgaaaatgac ttctcttcaa cacttttgat tagtcctata tctacaattt ctttttcacc
      121 atctatgatg tttgaaaaga cgatcaactc tattatgttg gctaaattaa ttttaaatga
      181 atatacagca ttatctgaag ataaaaaaga aacattgaca aatattgttc ttttctgtga
      241 acattataac cgtctatcag aagaaaaaat tttactccca gaaaactggg cagaattaag
      301 aaagttggta gagtatggga ttatttaaca cactttccaa gtcaaaatta gttaagtcca
      361 gtttctggta tactataggg aattttttta ttaagggaat ttcattcata actattccta
      421 tttttgtaaa tattactact caagcagaat atggtttgat taataatttc acttcatttg
      481 tatcaatttt ttcgattctt gttgggctaa gcctaaattc ggcaattaat aatgcaagtt
      541 ttgactataa agatgacata aatgaattta tgtcatcggt actttctcta gctacacttt
      601 cgttaattgt gttcttatta atagggaaca tatatttttt gttttcaaat gctttttttg
      661 atatgaacca acttgtattt aatttgatgt tattacaaag ttttgctagt tttgtaatta
      721 gttatatcca gtcttatttt acgattaact ttaaccattt taaatatttg attttatctt
      781 ttatatctac ttttttgaat attgcaatct ctgtagcttt gatgcagaca ttatattttg
      841 atgataaata tgttggcaga gtagtcggcg gaacactgtc atttgtgttg ataggtttag
      901 taatttattt aagaataatg ataaagggtg ctcatttttt tgataaaaaa cagtggagat
      961 acgctttaaa aatctctttg ccgctaatcc cacattcctt agcaaatata gttttagcac
     1021 aattcgatag aattatgatt aattcttatt taggtcctgc tgcgggggga atatattcct
     1081 acattagtaa cataggtatt attctaagcg taatatggat gtctactaat aacgcctggg
     1141 ttccatggtt ctatggtgag atgagtaaaa ataatgaaaa aaaagtgaag aaagttagta
     1201 actattattt agtagtcttt actttgatta cgatgattgt aatggtagtt tcgattgatt
     1261 tagcaaaatt tatggctccg aaagactatt attctgggtt atctttagtg gttccgataa
     1321 ccttaggcta tttctttcag tttttatata gtttaccagt taatgctgaa ttttatgaga
     1381 aaaaaacatc gtttattgcg attggtacag tagcaagtgc tatagttaat atactattaa
     1441 acatgatttt cataccta
//
LOCUS       DACDKM010000226         1437 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20834_2016
            SAMN12640370-rid9891663.denovo.231, whole genome shotgun sequence.
ACCESSION   DACDKM010000226 DACDKM010000000
VERSION     DACDKM010000226.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640370
            Sequence Read Archive: SRR10040891
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1437)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1437)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742835.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742835.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:04:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,012
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 29 of 75
            Pseudo Genes (incomplete)         :: 33 of 75
            Pseudo Genes (internal stop)      :: 19 of 75
            Pseudo Genes (multiple problems)  :: 5 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1437
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640370-rid9891663.denovo.231"
                     /isolate="E20834_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            105..1406
                     /locus_tag="IV334_002848"
     CDS             105..1406
                     /locus_tag="IV334_002848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HAP8944848.1"
                     /translation="MPDLHHIKLLLGIKDKNIFITNVESKSIKNIKSLVVSATLSKEI
                     RRCPLCKQMNHEGMIVKNGKKKSLIQLNKCANQLTYLALAKQRYHCRGCHTYFTANTY
                     IVDRNCFIAKQVRYKILEELTEKQAMTTIAKHCGVSWSTVSRTLASLIPMTKVKRNWL
                     PRCLLMDEFRSLKNQVGPYSFSCMDGDTGKLLDILPSRKKKALVSYFMQFERRARLKV
                     KVIVTDMNVSYASLIKECFPKAKLVVDRFHIVKHLIRKFEDIRVRIMKSFDRNDPTQA
                     KYYRQLKALSRLLIKRQDKLVYDKWTKWRNFGWAYLTESEVVDRLLSISDELRIAYFY
                     YQEILQAFHDKEADTFFKLVRTMPNSVPKELHSIKKAFINYESGIRLALELPYSNAKI
                     ENLHTHIKALKRVAYGFRSFRKMKTRIFLLNNLITYESKNI"
BASE COUNT          473 a          288 c          264 g          412 t
ORIGIN      
        1 gatagaaaat cattctatcg gtcccttttg agcatgaaaa aacaaaacat acagaagctc
       61 cgttagaatt gaatcaccac aacacaatca aaggagcgtt ctgtatgcct gatcttcatc
      121 atatcaaact actacttgga atcaaagata aaaacatttt catcacaaat gtggaatcga
      181 agagcattaa gaatatcaag agtcttgttg tttctgcgac gttaagtaaa gaaattcgtc
      241 gctgtccttt gtgtaaacag atgaatcatg aaggcatgat cgttaagaac ggaaagaaaa
      301 aatcgctcat tcaacttaat aaatgtgcga accaactgac ttatcttgct ttagcgaaac
      361 aacgctacca ctgtcgtggc tgccacacct attttacggc gaatacctac attgtcgata
      421 gaaactgctt tatcgctaaa caagtgcgct ataaaatttt agaagagcta actgaaaaac
      481 aagcaatgac gacaattgct aagcattgcg gcgtttcttg gtcgaccgtt tctcggacac
      541 tcgcttctct gatccccatg acaaaagtaa aaagaaattg gttaccgcgt tgtttattaa
      601 tggatgaatt tcgttcactg aaaaatcaag tcggtcccta ctccttttct tgtatggatg
      661 gcgatactgg aaaattactg gatattcttc cttcacggaa gaaaaaggct ctcgtttctt
      721 attttatgca atttgaacga cgcgcacgat tgaaggtaaa ggtcatcgtg acggacatga
      781 atgtttctta tgcctctctg ataaaggaat gttttccaaa ggcaaaactc gttgtggatc
      841 gttttcacat tgtcaaacat ctcattcgta aatttgagga tattcgcgta cgaatcatga
      901 aaagctttga tcgaaacgat ccaacacaag ccaaatatta tcgacaatta aaggcgttgt
      961 ctcggcttct gatcaaacga caggacaagc ttgtttatga caaatggacc aaatggcgca
     1021 attttggttg ggcgtatctc accgaaagtg aagtggtgga tcgtctgctt tcgatctctg
     1081 acgaattacg aatcgcctat ttttactatc aagaaattct tcaagcgttt cacgacaaag
     1141 aggcggacac tttctttaaa cttgtaagaa cgatgcccaa ttccgttcca aaagaactgc
     1201 attctataaa aaaagctttt ataaactatg aatccggcat tcgtttagcc ctcgaattgc
     1261 cttattcaaa tgccaaaata gaaaatctgc atacccacat caaagcattg aaacgagtcg
     1321 cttatggctt tcgaagcttt cggaaaatga agaccagaat tttcttacta aataatctga
     1381 ttacctatga atcaaaaaac atctaaagta gaaaaggaat tctaccttag atgttct
//
LOCUS       DACDKM010000227         1424 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20834_2016
            SAMN12640370-rid9891663.denovo.232, whole genome shotgun sequence.
ACCESSION   DACDKM010000227 DACDKM010000000
VERSION     DACDKM010000227.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640370
            Sequence Read Archive: SRR10040891
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1424)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1424)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742835.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742835.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:04:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,012
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 29 of 75
            Pseudo Genes (incomplete)         :: 33 of 75
            Pseudo Genes (internal stop)      :: 19 of 75
            Pseudo Genes (multiple problems)  :: 5 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1424
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640370-rid9891663.denovo.232"
                     /isolate="E20834_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(150..1337)
                     /locus_tag="IV334_002849"
     CDS             complement(150..1337)
                     /locus_tag="IV334_002849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element IS16 family transposase"
                     /protein_id="HAP8944849.1"
                     /translation="MTQVHFTFESEEIQAIINESGANDTAKTLMTIMFNQLMEEQRNQ
                     YIQANAYERSEERQSQRNGYYDRSFTTRIGTLELHVPRTRDGKFSPTIFERYQRSEKA
                     LIAAMIEMVISGVSTRKVTKTVELLTDGATVSKSFVSNLMKQLDPFVFEWRNRSLEGS
                     EYPFFMCDALYMKVRENHRIVSKGVYIGIGIDSDGRRTILGFDVQDGESEDNWDTVFQ
                     SFVQRGLFGVKLVISDAHKGLVKAVRKNFLGASWQRCQAHFLRNIFDKLPKKVSSDVK
                     DELKSIFKASELELTRERKEHFLEKYGCDSKLSAACDILENGFEDAIQILSFPENIRR
                     RIRTTNVLERLNEEIRRRERVIRIFPNINSITRIIGTLLMEKDTEWLASPRKYLEFNS
                     NNI"
BASE COUNT          414 a          309 c          249 g          452 t
ORIGIN      
        1 taaaagttca acctcatcaa aagcacaatg ttttgcgttg tgaacctaac tgccatgcct
       61 ttctttctga ggagagaggt caggagcagt agcgcagcga ctcttgatgt ctctcctcag
      121 aaagaagaga ctcgcacagg ttcatggtgc taaatgttat tcgaattaaa ttctaaatat
      181 tttcgtggag aagccagcca ctcagtgtct ttttccatca aaagtgttcc aattatccga
      241 gtgatggaat tgatatttgg aaaaatccga atcactcgtt ctctccggcg aatctcttcg
      301 ttcaaccgtt ccaacacatt ggttgtacga atccttcggc ggatattttc aggaaaagac
      361 aaaatttgaa tagcatcttc aaagccgttt tctaaaatgt cgcaagcagc acttaatttt
      421 gaatcacaac catatttttc caagaagtgc tcttttcttt cacgtgtcaa ttcaagttca
      481 gaggctttaa agatactctt caactcgtct tttacatcag aagaaacttt ctttggcagc
      541 ttatcaaaaa tatttctcaa aaagtgggct tggcatcttt gccagctcgc acctaaaaag
      601 ttcttgcgaa cagccttaac taaccctttg tgtgcatcag aaatgaccag ttttacaccg
      661 aacaaacctc gttgaacgaa cgattgaaaa acggtatccc aattatcttc cgattcgccg
      721 tcttgaacat caaaaccaag aatcgtacgt cgtccatcag aatcaatgcc gataccaata
      781 tatacacctt ttgagacaat acgatgattt tcccttactt tcatatacag ggcatcacac
      841 atgaaaaatg gatattccga gccttctagg ctccggtttc tccactcaaa aacaaatgga
      901 tccaactgtt tcatcagatt tgaaacgaat gatttagaaa cagtcgcacc atccgttagc
      961 agttcaactg ttttagttac tttgcgagtg gagacaccag aaatgaccat ttcaatcatg
     1021 gcagcgatca aagctttttc gcttcgctga tatctttcaa agatagtagg actaaactta
     1081 ccgtctctgg ttcgtggaac atgaagttct aacgtaccaa ttcgagttgt aaacgaacga
     1141 tcgtagtaac catttctttg tgattgccgt tcttctgaac gttcataggc atttgcctga
     1201 atatattgat ttcgttgttc ttccatcagc tgattaaaca tgatagtcat aagtgttttt
     1261 gcggtatcat tcgcaccgct ttcgtttata attgcttgaa tttcttcgct ttcaaaagta
     1321 aaatgtactt gagtcattga tatcagtcct attcgagtat gttttctgca aaaaacattg
     1381 tactcctaac tgagatcaat ggcttttttg atgcttcact ttta
//
LOCUS       DACDKM010000228         1418 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20834_2016
            SAMN12640370-rid9891663.denovo.233, whole genome shotgun sequence.
ACCESSION   DACDKM010000228 DACDKM010000000
VERSION     DACDKM010000228.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640370
            Sequence Read Archive: SRR10040891
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1418)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1418)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742835.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742835.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:04:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,012
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 29 of 75
            Pseudo Genes (incomplete)         :: 33 of 75
            Pseudo Genes (internal stop)      :: 19 of 75
            Pseudo Genes (multiple problems)  :: 5 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1418
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640370-rid9891663.denovo.233"
                     /isolate="E20834_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..106
                     /locus_tag="IV334_002850"
     CDS             <1..106
                     /locus_tag="IV334_002850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HAP8944850.1"
                     /translation="ASKRNNIPRKSLDYRTPLEVFLSYVSIDDLSNLI"
     gene            970..1182
                     /locus_tag="IV334_002851"
     CDS             970..1182
                     /locus_tag="IV334_002851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8944851.1"
                     /translation="MVKAEVGRSYICKPIGVSHELIGCIERTYINTAVVYVESYDQRD
                     YQLINESKYRMLVKFCDISAPAELVS"
BASE COUNT          523 a          213 c          237 g          445 t
ORIGIN      
        1 cgcatctaaa cgaaataata ttcctagaaa atcactggat tatcgaacac ctttggaagt
       61 atttttgagt tacgtaagta ttgatgatct gtctaactta atttgacaat taagattata
      121 ttaaatatta tacagataat aacggaatat tatcatcgaa acagctaaca aaattgtcat
      181 aaaaaacgag atggttgata ttagaacttg tgtttttgtt taggtctaga gataaagaga
      241 gacaatttcc aaatatatgt aatgaataca aaaagacgtt tgtcgcggat taccgttaca
      301 aacgtctttt attgattttc cgagatctca tcagtcgcca tttggaatgg ttcactcctt
      361 atctagattc gtttaatgct cggactgctt gttcgattgt aataccaaac gcaacgttat
      421 tttcgttttg tgcatcaatc gccataaaac ggttagaagt tgtatcaaaa gctacgcggt
      481 attgcatatt tgtttttgta aacgtcataa aattcatctc ctttgtaatg taatttagtt
      541 cttgaaagtt tcctttaaac tttcgaactt ataccaagtt tagcatcgta cgttttttga
      601 tgcaattaaa aagcatttat cattttcctt atttattttt tcttaaatgg tttagctatt
      661 ttcttttaat ccgataagta gcccaatcat caaggttaca aatactattg cttgaatata
      721 aatcatatgc tcacctcttt ataaataact tagcaatata aagaaaaaaa cgcacgaaca
      781 acataggaat ataaacaaac gtttttgtca caacgtctaa gatcgtgtta aaaaatagtt
      841 atctaaaaag cagaaagata ctcaaaataa aaaataaaaa aatggataaa aacgttttca
      901 aatacaaggt aatttgttat tataaaataa agaaaaaaca aaataaaaat aaagaaaaga
      961 gggaatgaaa tggtaaaagc agaagtagga agatcttata tatgcaaacc gatcggagta
     1021 tctcatgaac taatcggctg tattgaacgt acatatatca acacggctgt cgtgtatgtt
     1081 gaaagttatg accaacgaga ctatcaactt atcaatgaaa gcaagtatcg catgctcgta
     1141 aaattctgtg atattagcgc acctgctgaa ttggtttctt aaacagtatt tttgagaatt
     1201 cgcaagaact cccttataat ataaataaag acagaaggga gcgagaagat atgccaaaaa
     1261 gaaaaagttc agggaaaatg ggaattattc ttgcagtgat ctctgcaatt cctgctattt
     1321 tagctattgt tcgtgcgatg aaggaatcaa gagaaagtta agcacacatt atgagcgtca
     1381 aacaaaaaac gcatatacct gcggtggcag gttatgcg
//
LOCUS       DACDKM010000229         1409 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20834_2016
            SAMN12640370-rid9891663.denovo.234, whole genome shotgun sequence.
ACCESSION   DACDKM010000229 DACDKM010000000
VERSION     DACDKM010000229.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640370
            Sequence Read Archive: SRR10040891
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1409)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1409)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742835.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742835.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:04:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,012
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 29 of 75
            Pseudo Genes (incomplete)         :: 33 of 75
            Pseudo Genes (internal stop)      :: 19 of 75
            Pseudo Genes (multiple problems)  :: 5 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1409
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640370-rid9891663.denovo.234"
                     /isolate="E20834_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(138..428)
                     /locus_tag="IV334_002852"
     CDS             complement(138..428)
                     /locus_tag="IV334_002852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8944852.1"
                     /translation="MGEKKIKGAGILLVMSKALFLLPCTFMFIVLCYFKRRKKWCWID
                     VLLFLPFFPLIYFLEEIWDDMACDSAFLFSHSLGLMISLTIFAIVLFFLNKK"
     gene            complement(546..806)
                     /locus_tag="IV334_002853"
     CDS             complement(546..806)
                     /locus_tag="IV334_002853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8944853.1"
                     /translation="MGVDHLIVLSKLLFWIPFIIMTSAFFYLFKRPNKYLLLSACPMI
                     YFIHKIRGFYYYDSGISLFCYEIGLLASAIFFIFVLEYFRKK"
     gene            complement(943..1356)
                     /locus_tag="IV334_002854"
     CDS             complement(943..1356)
                     /locus_tag="IV334_002854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HAP8944854.1"
                     /translation="MANVPSIRDMFPDFNDVFSPAFNDFLGVSSYPKVDLVENEKEYK
                     LTADMPGCDKEDTTVEYSDNTLTISANHESHTEDKEDGNYVRKERHSVSYKRSFYLPN
                     VDEEKITGTFKNGVLKLVLPKTAYQPKETKKIELN"
BASE COUNT          517 a          234 c          208 g          450 t
ORIGIN      
        1 tttgttacta tgaataacat aataagaaag taaagtggat tgatttgaga tctttttata
       61 cttcaaaaaa gataacaccc aactttcaaa aatatgtttt ggaagttggg acaaacgagt
      121 taatacattt taatacatta ttttttattc aaaaaaaata gcacaatggc aaagatcgta
      181 agagagatca taagccccaa tgaatgacta aaaagaaaag cagaatcaca tgccatatca
      241 tcccatattt cttcaagaaa ataaataagc gggaaaaagg gaaggaatag tagaacatcg
      301 atccagcacc atttttttct tcttttgaaa taacataaaa caataaacat gaacgtgcag
      361 ggaagtaaaa acaaagcttt actcataact aacaaaatac cagctccttt tatttttttc
      421 tcccccacat tcaaagagta acaatggtat gtacttaata atagaaaaat ggatcaactt
      481 gtatgaataa cagctgaaaa gatctgcacc aaataaaaaa cttccagcca aaactgactg
      541 gaagtttatt tttttctgaa atattctaac acaaatatga aaaagatggc cgaagctaat
      601 aagccgattt cgtagcaaaa caatgagata ccagagtcgt aataataaaa tcctctaatc
      661 ttatggataa aatagatcat cggacaagca gataagagta agtatttgtt aggccttttg
      721 aataagtaaa agaaagcact ggtcattatg ataaatggta tccaaaacaa aagtttactt
      781 aagacaatca aatgatcaac tcccatattt tttaccaaga gagatcataa catctttatc
      841 gtaaaaacaa aatatttttt tgctcatttt gaaaatttaa acatactcgt tcttatccaa
      901 atatataaaa atcgcccctc attcatcgag aggcgataag ttctaattta actcaatttt
      961 ttttgtttct tttggctgat aagctgtttt aggtaatacc aatttcagca caccgttttt
     1021 aaaggttcca gtgatttttt cttcatcaac attaggaaga tagaatgaac gtttgtaaga
     1081 gaccgaatgt ctttctttcc gtacatagtt gccatcttct ttatcttctg tatgggactc
     1141 atgattggcg gagatcgtca aagtattatc tgaatattca actgtcgtgt cttctttgtc
     1201 acagccaggc atatcagcag ttagtttgta ttctttttca ttttcgacta aatctacttt
     1261 tggataactt gaaacaccta aaaaatcatt gaatgctgga ctaaacacgt cattgaaatc
     1321 agggaacatg tcgcgaatgc taggtacatt tgccattttt gatcatcctt tcaataagta
     1381 ttttttattt atccctattt acgtaataa
//
LOCUS       DACDKM010000230         1367 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20834_2016
            SAMN12640370-rid9891663.denovo.235, whole genome shotgun sequence.
ACCESSION   DACDKM010000230 DACDKM010000000
VERSION     DACDKM010000230.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640370
            Sequence Read Archive: SRR10040891
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1367)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1367)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742835.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742835.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:04:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,012
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 29 of 75
            Pseudo Genes (incomplete)         :: 33 of 75
            Pseudo Genes (internal stop)      :: 19 of 75
            Pseudo Genes (multiple problems)  :: 5 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1367
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640370-rid9891663.denovo.235"
                     /isolate="E20834_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..1305
                     /gene="acm"
                     /locus_tag="IV334_002855"
     CDS             <1..1305
                     /gene="acm"
                     /locus_tag="IV334_002855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Acm"
                     /protein_id="HAP8944855.1"
                     /translation="PEQINFENHSEAWFKEFNKPAVDGESFNHTVKNISASGGVNGTV
                     RGELKIFKYINDTEIGIPNVTFELRRADEQPIQGQSSILLTSNEQGEASIKGLQVGDY
                     VVKEKEAPNWIDFDPLSSNELKFSINENDTEGVSLPIYNKKKVTNITATKIWNGGTTP
                     RPSIYFKLFRASTNNWEPVPDAETKRLDNGITSVTWEDIQQYDDSGNEYTFKVQEVDQ
                     NGNDYVPSGYQKIENGLVVTNENKEVISVSGQKTWEDNENQDGKRPTTITVNLLADGQ
                     PIQHKEVSEKDDWRYRFTNLPKYRDGQEIIYTVTEDNVPEYSTTIDGYNIKNSYTPEK
                     TNISIPGSHITPWKNFPEKTEREKIRNLFSQKLQIFAHTGESSEQRSNYISKRAVPKQ
                     ITNKSKSILPKTSSKKSSFAVIIGLLIVTICAGIFLQKYKKV"
BASE COUNT          553 a          219 c          258 g          337 t
ORIGIN      
        1 ccagaacaaa taaattttga gaatcattcg gaagcttggt ttaaagaatt taataaacct
       61 gctgttgatg gagaaagttt caatcataca gttaaaaata tttcagcttc tggtggagtc
      121 aacggtacag ttagaggcga attgaagatt ttcaaatata tcaatgacac agagattggt
      181 atcccaaatg tgactttcga attaagacga gcagatgaac agcctattca aggacagtct
      241 agtatcctgt tgacttcgaa tgaacaggga gaagctagta tcaaaggcct gcaagtagga
      301 gactatgttg taaaagaaaa agaagcaccc aattggattg attttgatcc actaagttca
      361 aatgaactca aattctccat aaacgaaaat gatacagagg gagtcagttt acctatttat
      421 aacaagaaaa aggttacgaa catcacagca actaaaattt ggaacggtgg aaccactcca
      481 agaccgagta tttattttaa attattcaga gcaagtacaa acaattggga accagttcct
      541 gatgcagaaa caaaacgatt agacaatgga ataacttcag tgacttggga agatatccaa
      601 cagtatgatg atagtggaaa cgaatatacg tttaaagtgc aagaagtaga tcaaaacgga
      661 aatgattatg tcccttcagg ttatcaaaaa attgaaaacg ggctagtcgt tacaaatgag
      721 aataaagaag tcatttcagt ctcaggacaa aaaacatggg aagacaatga gaaccaagac
      781 ggcaaacgac cgacgacgat cacagtaaat ctattagccg acggtcaacc gatccaacac
      841 aaagaagtaa gtgaaaaaga tgattggcgc tatcgtttca caaatctgcc aaaatacaga
      901 gacgggcaag aaatcatcta tactgtaacg gaagacaacg tgcccgaata cagtacgacg
      961 attgacgggt ataacatcaa aaacagttat acaccggaaa aaacaaatat atctatacca
     1021 ggtagccata taacaccgtg gaaaaatttt ccagagaaga cagagagaga gaaaattaga
     1081 aatctattct ctcaaaaatt gcaaatattt gctcatacag gagaatccag cgaacaaaga
     1141 agcaattata ttagtaagag agctgtacct aaacagataa caaataaatc aaaaagcatt
     1201 cttcctaaaa caagtagtaa aaaatctagt tttgctgtaa tcattggttt attgatagta
     1261 actatctgtg ctgggatatt cttacaaaaa tataaaaaag tttagttaga atataatatt
     1321 ttttgttaaa tatgactgaa atcaaaagaa taaaattccg atttata
//
LOCUS       DACDKM010000231         1364 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20834_2016
            SAMN12640370-rid9891663.denovo.236, whole genome shotgun sequence.
ACCESSION   DACDKM010000231 DACDKM010000000
VERSION     DACDKM010000231.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640370
            Sequence Read Archive: SRR10040891
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1364)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1364)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742835.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742835.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:04:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,012
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 29 of 75
            Pseudo Genes (incomplete)         :: 33 of 75
            Pseudo Genes (internal stop)      :: 19 of 75
            Pseudo Genes (multiple problems)  :: 5 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1364
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640370-rid9891663.denovo.236"
                     /isolate="E20834_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            82..>1364
                     /locus_tag="IV334_002856"
     CDS             82..>1364
                     /locus_tag="IV334_002856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3-like element ISEfa5 family transposase"
                     /protein_id="HAP8944856.1"
                     /translation="MNDSIKKMLRLIEKDLMITEVSYETFQKKKTLIVDAVFSPAPHT
                     CRNCGSTVVDGNGKVIVVKNGKKETIVRFEQYNHMPLVMRLKKQRYTCKNCRTHWTAQ
                     SYFVQPRHSIANHVRYKIASLLTEKVSLSFIAKNCQVSLTTVIRTLKEFKSYLPKQSK
                     RILPRVLMVDEFRSHASIEDKMSFICADGETGQLIDVLPTRKLPRLTSYFLGCANPEE
                     VKFLVTDMNAAYFQLTKRILPNAKVVIDRFHIIKHMNQAFNELRIREMNELRKVGQKS
                     QAEKLKKNWRFLLKNRANINHYEYKTWKSFRAPKYPFLTEAMMIDRLLGFSTSLKEAY
                     PYFHELVEAFRDKDPDLFFSLLAELPETLDDGFREKLQNLLTYEEGITNAMIYPYTNG
                     KIEAKNTHIKTMKRVSYGFKSFENMRIRIFLINQL"
BASE COUNT          463 a          255 c          259 g          387 t
ORIGIN      
        1 attttaggta ttaaaaaaca tatcgttctc tagtatgatt aaatcacgac aaaataaaca
       61 actgagagga cgatatgcta tatgaatgat tctatcaaaa aaatgctgcg attaatagag
      121 aaagatttga tgattacaga ggtctcttac gagacctttc agaagaaaaa gacgttgatc
      181 gtcgatgctg ttttctcacc tgctccccat acctgtagaa attgtggttc tactgtggta
      241 gatggaaacg ggaaagtaat tgtagtgaaa aatgggaaga aggaaacgat tgtccgcttt
      301 gaacaataca atcacatgcc tttggttatg cgcttaaaaa agcagcgcta tacctgtaaa
      361 aactgtcgaa cccattggac ggctcaaagt tattttgttc aacccagaca ttcaatcgcg
      421 aatcatgtca gatataaaat tgcttcttta ctgactgaaa aagtatcttt atcttttatt
      481 gcgaaaaatt gtcaggtatc tttgaccacc gttattcgta cgttgaaaga gtttaaaagc
      541 tacttaccaa agcaatctaa gaggattctc cccagagtat tgatggttga tgaatttcgt
      601 tcgcacgctt ccatagaaga taaaatgagc tttatttgcg cagatggcga aacaggacaa
      661 ttaattgatg tcttgcctac gcgtaaattg cctcgattaa caagctattt cttaggctgc
      721 gctaatcctg aagaagtaaa atttttagtc acagacatga acgccgccta cttccagctt
      781 accaaacgta ttctgccaaa tgcgaaagta gtgattgatc ggtttcatat tatcaaacac
      841 atgaatcaag cctttaatga gttgcgtatc cgtgaaatga atgaacttcg taaagtagga
      901 cagaaaagtc aggcagaaaa actgaaaaag aactggcgct ttttgctaaa aaatcgtgca
      961 aacatcaacc attatgaata caaaacatgg aaaagttttc gagcaccaaa atacccattt
     1021 cttacggaag cgatgatgat tgatcgattg cttggctttt ctacgtccct gaaggaggcg
     1081 tatccctatt ttcatgaatt agttgaagcc tttcgagaca aagaccctga cttatttttc
     1141 tccttattgg cagaacttcc cgaaacgtta gatgacggct ttcgggaaaa gctccaaaac
     1201 cttctgacct atgaagaagg catcaccaac gcgatgatct atccttacac caatggaaaa
     1261 atagaagcga agaataccca tataaagaca atgaaacgag tatcctacgg atttaaatca
     1321 tttgagaaca tgagaattag aatctttttg atcaatcaat taat
//
LOCUS       DACDKM010000232         1364 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20834_2016
            SAMN12640370-rid9891663.denovo.237, whole genome shotgun sequence.
ACCESSION   DACDKM010000232 DACDKM010000000
VERSION     DACDKM010000232.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640370
            Sequence Read Archive: SRR10040891
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1364)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1364)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742835.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742835.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:04:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,012
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 29 of 75
            Pseudo Genes (incomplete)         :: 33 of 75
            Pseudo Genes (internal stop)      :: 19 of 75
            Pseudo Genes (multiple problems)  :: 5 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1364
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640370-rid9891663.denovo.237"
                     /isolate="E20834_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(68..688)
                     /locus_tag="IV334_002857"
     CDS             complement(68..688)
                     /locus_tag="IV334_002857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit delta"
                     /protein_id="HAP8944857.1"
                     /translation="MEISVFEGANKSELSMIEVAHAILEQRGDVMDFSDLANQIQNYL
                     GKSDSEIRDSLAQFYTDLNIDGSFISLGDNRWGLRSWYAIDSIDEEVNHGMDEEDEDT
                     PRRRKRKKVNAFINDDEDAIDYNDDDPEDTDLTEEDDDDLFDDDDDEDEEIAAYNSDL
                     QEIGADDPTDDEEDLPGNIEEDLSIIDEDDDDEDYEEEEFSDSEKE"
     gene            complement(764..1201)
                     /locus_tag="IV334_002858"
     CDS             complement(764..1201)
                     /locus_tag="IV334_002858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1934 domain-containing protein"
                     /protein_id="HAP8944858.1"
                     /translation="MELSQGTPISIKLRTKVKQQGEVQDFYFDLKGQMVTIGDTLYIR
                     YKEIQEETGDEIPVTIKLVPDGHVQLIRAGEMRMRLKFGYKERLETSYRTPYGMIQIA
                     TFTKELHVSLKDRPTGGKVRIDYDLFMGPEKIGEYYLTLDFTA"
BASE COUNT          361 a          321 c          204 g          478 t
ORIGIN      
        1 cgtgtcacac ctccttattc tgataggctg atcgtctctg tcacaacctc tcgtaagaaa
       61 ataatgttta ttctttttca gagtcagaaa attcttcctc ttcataatct tcatcatcat
      121 cgtcttcgtc tatgatactt aaatcttctt cgatatttcc tggcaagtct tcttcatcat
      181 ctgtcgggtc atctgctccg atttcttgca agtcagaatt ataagcagcg atttcttcat
      241 cttcatcatc atcgtcatca aacagatcat cgtcatcttc ttctgtcaga tctgtatctt
      301 ctgggtcatc gtcattgtaa tcgatcgcgt cttcatcatc attgataaag gcgttgactt
      361 tcttgcgttt acggcgacgt ggagtatctt catcctcttc gtccattcca tggtttactt
      421 cttcatcgat cgaatcgata gcataccatg aacgcaagcc ccaacgatta tcccctaatg
      481 agataaagct gccgtcaata ttcaagtcag tataaaattg tgcgagtgaa tcacgaattt
      541 cactgtctga tttcccaaga tagttttgga tctggttagc taaatcagaa aaatccatga
      601 catcgccacg ttgttctaag atcgcgtgtg ctacttcgat cattgataat tcgcttttgt
      661 ttgcaccttc aaatacacta atttccaatc aggacacgtc ctttcacagt ctactcctta
      721 tcatacaaaa tcagacacaa aaaatcaaac ttttctttta tatttacgct gtaaaatcca
      781 aggtcaaata atattcgccg attttttccg gtcccatgaa cagatcataa tcgattcgaa
      841 ccttccctcc tgtgggacgg tctttcaaac taacatgcag ttctttcgta aaagtagcaa
      901 tctggatcat accgtatggt gttcgatagg atgtttctaa ccgttcttta tagccaaatt
      961 tcaaacgcat ccgcatttct cctgcacgga tcagctgtac atgaccatct ggaactaatt
     1021 tgatcgttac aggaatctca tcgcccgtct cctcctgaat ctctttataa cgtatgtaca
     1081 gcgtatcgcc aattgtcacc atttgccctt tcaaatcaaa ataaaaatct tgcacttccc
     1141 cttgctgttt tacttttgta cgcaatttga ttgaaatcgg tgttccttgt gataattcca
     1201 ttattcattc tcctttactt ctcatagcta cattgttcct agtttctttt gttttgtcaa
     1261 ccaacttgtc catttttaca gaaaaccttt tttccaaaac aactctttga ttttagagta
     1321 taatattgta gaaaatatga atttatacaa aggagccggt tgaa
//
LOCUS       DACDKM010000233         1361 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20834_2016
            SAMN12640370-rid9891663.denovo.238, whole genome shotgun sequence.
ACCESSION   DACDKM010000233 DACDKM010000000
VERSION     DACDKM010000233.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640370
            Sequence Read Archive: SRR10040891
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1361)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1361)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742835.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742835.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:04:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,012
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 29 of 75
            Pseudo Genes (incomplete)         :: 33 of 75
            Pseudo Genes (internal stop)      :: 19 of 75
            Pseudo Genes (multiple problems)  :: 5 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1361
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640370-rid9891663.denovo.238"
                     /isolate="E20834_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            24..>1361
                     /locus_tag="IV334_002859"
     CDS             24..>1361
                     /locus_tag="IV334_002859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HAP8944859.1"
                     /translation="MSYSEFTKELLDILDLNLTFHEDAFRKERINDETCFVFDGTLTY
                     QPEECFHCHYQNKQTIIKWGWKKVSILLNDVSNYKTIVRINKQRFKCKHCGKTFLAED
                     SVSDRRCSIARRVKQAILELLSEPISMSLIARMKHISPTTVIRILRSLRPKTVSLNQP
                     LPEVVCFDEFKSVKNVSGAMSFVMMDGKTHQLIDIVENRQLNPLRDYFLRYPRKVREQ
                     VRLVVSDFYTPYRTLVKECFPNARIVADRFHISQHIGRAFTNHRIQVMKTFKKGDRRS
                     KHLKKYWRLLQKNAWELNGQHRYWRPSFRDHLTEAEIVDRLLYYDDSLKRGYEVYQVF
                     LSAIRRQDVPEFVALLKEDYKELPEHYQPVFTTFKKYRTEIKRALRVPYSNGPIECLN
                     NHIKVLKRIAYGFRNFQNYRERIFLYRGKYFKKTKNTTQLTKARTTTRLDKIAV"
BASE COUNT          429 a          302 c          270 g          360 t
ORIGIN      
        1 accattcaaa ggagtgtttc aagatgtcct attcagagtt taccaaagag ttactagata
       61 ttctagacct aaatcttacc tttcatgaag atgcctttcg caaagagcgc attaatgacg
      121 aaacatgttt tgtttttgat ggcacattga cctatcaacc agaggaatgc ttccattgcc
      181 actatcaaaa taagcaaacg atcattaaat ggggctggaa aaaagtatcc atcttattga
      241 atgatgtcag taactacaag accattgtgc ggattaacaa acagcgcttc aaatgtaaac
      301 actgtggaaa gactttttta gcagaagatt cagttagcga tcgtcgctgc tccattgccc
      361 gcagagtcaa acaagccatt cttgagctat taagtgagcc tatttctatg tctttgattg
      421 cacgtatgaa gcacatttct ccgaccactg tcattcgaat acttcgcagc cttcggccta
      481 aaaccgtttc tttgaaccag ccgttaccag aagtggtgtg tttcgatgaa ttcaaatcgg
      541 tgaaaaacgt ctcgggagcc atgagctttg tcatgatgga cggcaagacc catcaactga
      601 ttgatattgt ggaaaatcgg caactcaatc ctttacgcga ctatttcttg cgctacccta
      661 gaaaagtccg tgaacaagta cgtctagtgg tttcagattt ctatacaccc tatcgcacat
      721 tggtgaaaga gtgttttccg aatgcccgaa tcgtcgccga tcgctttcat attagccaac
      781 acatcggtcg ggcctttacc aaccaccgaa ttcaagtaat gaagaccttt aaaaaagggg
      841 atcgtcggtc gaagcactta aagaaatact ggagactact tcaaaagaat gcgtgggaat
      901 tgaatggtca gcatcgctac tggcgtccgt catttcgaga tcatctgaca gaagcggaga
      961 tagtcgatcg gctattatat tatgatgact ccttaaagcg aggctacgaa gtctaccagg
     1021 tttttctttc cgctatcaga agacaagatg ttcctgaatt cgtcgcgctc ctaaaagaag
     1081 actacaagga gttgccagag cactatcaac cagtgttcac gaccttcaag aaatatcgaa
     1141 cagaaatcaa acgagcgttg cgagttccct attccaacgg ccctatagaa tgtttgaaca
     1201 accatatcaa agtactaaaa cgaattgcct atggctttag aaactttcaa aactaccgag
     1261 aacgaatctt tttatatcga ggaaaatatt tcaagaaaac taagaacact acccaactga
     1321 ctaaagccag aacaacaaca cggctagaca agatagctgt c
//
LOCUS       DACDKM010000234         1355 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20834_2016
            SAMN12640370-rid9891663.denovo.239, whole genome shotgun sequence.
ACCESSION   DACDKM010000234 DACDKM010000000
VERSION     DACDKM010000234.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640370
            Sequence Read Archive: SRR10040891
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1355)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1355)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742835.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742835.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:04:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,012
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 29 of 75
            Pseudo Genes (incomplete)         :: 33 of 75
            Pseudo Genes (internal stop)      :: 19 of 75
            Pseudo Genes (multiple problems)  :: 5 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1355
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640370-rid9891663.denovo.239"
                     /isolate="E20834_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            474..725
                     /locus_tag="IV334_002860"
     CDS             474..725
                     /locus_tag="IV334_002860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8944860.1"
                     /translation="MKEKPINKWSLYKNSMFGVVIFGVCAGLFLFILLQTVTLFSSNP
                     TYGWRDSIALGMFAGGYGFLLLFSILTLSPIHGLIQLWK"
     gene            759..1037
                     /locus_tag="IV334_002861"
     CDS             759..1037
                     /locus_tag="IV334_002861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8944861.1"
                     /translation="MEKHNITGSMYVDDQWFISTDEVKIAAIRRDYLQKIHKIKDPNG
                     KKARLIITLITSNGKKLKLAGSYDNVKKFEHWYLQKDSISLNKQFEKN"
BASE COUNT          479 a          189 c          255 g          432 t
ORIGIN      
        1 acaaataatt ggtttattct ggcaggcgta atgctttttt ctagtttcta tttatttagt
       61 aatgctctat tagcacagcg agggaaatat aagtttaaaa tattagatta gatattattt
      121 ttagagggga taaatataaa atgaaaagag gttatttata atggaaaaag tattaccaat
      181 taagagctac tgtattccaa ggcttttcat catagaattt caaaagtaaa gacatgtgtt
      241 tcacaaatag tgtctatcct ggctaaataa ttcttccact atctgcttta atttcagtag
      301 acttttcggc aagttatttg ctaatgagat gaatagagat tacaactcgc ctataaccgc
      361 ttaaaacaag cttttttaaa gtatagttag caagtcgtat tcatatgttc aaagaggagg
      421 gcttatgaac tttgaattca ttccgtgtga acagaacagt agggggaagt aaaatgaaag
      481 aaaagcctat aaataaatgg tcattgtata aaaattcaat gtttggagtt gttatctttg
      541 gtgtttgtgc tggattattc cttttcatat tattacaaac ggtgactctt ttctcttcta
      601 atcctactta tggatggaga gacagtatag ccttagggat gtttgcggga ggatatggat
      661 tcttattgtt gttctcaatt ctcacactaa gtccaatcca tggactcatt caactttgga
      721 agtaagaacg agccttaaac ttttcgttct cggaagaaat ggaaaaacac aacataacag
      781 gcagtatgta tgtggatgat caatggttta tcagcactga tgaggtaaaa atagctgcca
      841 ttaggagaga ttacctgcag aaaattcata aaataaaaga tcctaatggg aaaaaggcaa
      901 ggttaattat aactttgata acatctaatg gtaagaagtt aaaacttgca ggatcatatg
      961 ataatgttaa aaagttcgaa cattggtatc tgcaaaaaga cagcattagt cttaataaac
     1021 aatttgaaaa aaattaatca aagtcatcgg caacacgaat aatttgacat cttgatattg
     1081 tgatataagc aaaaacggca tttctgagac cgaacagcat aaaaaattta aatgttggaa
     1141 tttatataag caaaacccaa gaagctagat gcgatagagc atttaatttc ttggggtttt
     1201 gcttgggaat cagcatatca attaaaatga tcatctcggt atttcaaaag tgggtacgat
     1261 tacaaatagt tggagcaaaa tagtgggtaa aattaccatt ccagtgtctg tcttgcctct
     1321 ttttcgtcaa tcacaaatag acgagattca tagga
//
LOCUS       DACDKM010000235         1352 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20834_2016
            SAMN12640370-rid9891663.denovo.240, whole genome shotgun sequence.
ACCESSION   DACDKM010000235 DACDKM010000000
VERSION     DACDKM010000235.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640370
            Sequence Read Archive: SRR10040891
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1352)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1352)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742835.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742835.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:04:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,012
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 29 of 75
            Pseudo Genes (incomplete)         :: 33 of 75
            Pseudo Genes (internal stop)      :: 19 of 75
            Pseudo Genes (multiple problems)  :: 5 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1352
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640370-rid9891663.denovo.240"
                     /isolate="E20834_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            202..1077
                     /locus_tag="IV334_002862"
     CDS             202..1077
                     /locus_tag="IV334_002862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8944862.1"
                     /translation="MNNWLEYFPENVLERGYSYHLHGFVRHLNYTSKYLSATVSGTED
                     YKVVITWDEKTNMTCDCLYAIEGKKCKHMAAVLFAYEERPIKKSNYSLSELSSLVSSA
                     SSSLVRELLTEILIEHPQFIERFKVKMPFHAINYSDKLTTIIHKYDHIIKKNKNRKTA
                     KFIMEMRKFIQEAVESLIQQNAYLPAFELINEVIATLETFYWEPEDERTLLLIEDCYY
                     LWKELLAEAPHAEKRQMFSWFVCQVDHTDASYSKRYSIKILKEDFREKEFSNQKKKID
                     KKTKETVKKDDFNEK"
     gene            complement(1222..>1352)
                     /locus_tag="IV334_002863"
     CDS             complement(1222..>1352)
                     /locus_tag="IV334_002863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="xylulose kinase"
                     /protein_id="HAP8944863.1"
                     /translation="CQNFIQYTDAVEPLLQNVEKYHKIYQQCRKIYPATKEICHDL"
BASE COUNT          487 a          248 c          225 g          392 t
ORIGIN      
        1 ataaataaaa aacaaaaaaa gttccatctt taaaacacgc tattctttta ctttttctca
       61 cattctctaa gaaaagaaaa ggagaatgaa tttttgacaa ttcgagcaaa agtgattatc
      121 aaatatgtaa aatcagctac ttttttctta aacgtttctt tatactaaat ataaataagt
      181 gtttcatgaa aggagaacta gatgaataat tggcttgagt attttcctga aaacgttttg
      241 gaacgcggtt acagctatca tctgcacggt tttgtccgtc atttgaacta tacaagcaag
      301 tacttgtccg caacagtttc tggcacggaa gattataagg tagtgatcac ttgggatgaa
      361 aagaccaaca tgacttgtga ttgtctatat gccatagaag gaaaaaaatg caaacacatg
      421 gctgctgtct tattcgctta cgaagaacgt ccaataaaaa aatccaacta ctcactttcc
      481 gaactctcgt cccttgtttc ctccgctagt tcctctcttg tacgggagct tttaacagag
      541 attcttatag aacatccgca atttatcgaa cgcttcaaag ttaagatgcc atttcatgca
      601 ataaattatt cagataaatt aacaactatc atccataaat acgatcatat tatcaagaaa
      661 aacaaaaaca ggaaaacagc gaagttcatc atggagatgc gaaagttcat ccaagaagca
      721 gttgaatcgc tcattcagca aaatgcctat cttcccgcat tcgaattgat caatgaagtg
      781 attgctactc tcgaaacatt ctattgggag cctgaggatg aacgaaccct tcttctgata
      841 gaggactgtt attatttatg gaaagaatta ttagcagaag cacctcatgc agaaaaaagg
      901 caaatgttct cttggttcgt ctgccaagtc gatcatactg acgcctctta ttcaaaacgc
      961 tactccataa aaatcctgaa agaagacttt cgggaaaaag aattttctaa ccaaaagaaa
     1021 aaaatagata aaaaaactaa agaaacggta aagaaagacg atttcaatga aaaatagcaa
     1081 gtaaacgata acaaccatcc taaatccaca agaaatacag cagaccttgc tttaaaaaca
     1141 cgaatcatgc aaaggatgaa gtaaagaaga cttaacgcgt acccaatatt agtcatttga
     1201 ctattaggta cgcgttttgt tttataagtc atgacaaatt tccttcgttg ctgggtagat
     1261 tttccggcat tgttgataga ttttatgata cttttctaca ttttgtaaga gaggttccac
     1321 tgcatctgta tattggataa aattctgaca ac
//
LOCUS       DACDKM010000236         1342 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20834_2016
            SAMN12640370-rid9891663.denovo.242, whole genome shotgun sequence.
ACCESSION   DACDKM010000236 DACDKM010000000
VERSION     DACDKM010000236.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640370
            Sequence Read Archive: SRR10040891
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1342)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1342)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742835.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742835.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:04:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,012
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 29 of 75
            Pseudo Genes (incomplete)         :: 33 of 75
            Pseudo Genes (internal stop)      :: 19 of 75
            Pseudo Genes (multiple problems)  :: 5 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1342
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640370-rid9891663.denovo.242"
                     /isolate="E20834_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            395..1306
                     /locus_tag="IV334_002864"
     CDS             395..1306
                     /locus_tag="IV334_002864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HAP8944864.1"
                     /translation="MKKRFLALAIVLGTGLLSGCTNAGEKTAVSYKGGTISEQEVMDS
                     LKKMQGADSAVQQLIVYQVFEDKYGDDVSTKEIDSQYDQTKKQLGDSFDSQLKSAGYT
                     EQTFKDSIKQSLAFQEGLKKHIKLTDEDLKTAWESFHPEVEAQIIQVASEDDAKDVKK
                     AADKGDDFSKLAKDKSTDTTTKEDGGKVKFDSTTTTVPAEVKEAAFKLKDGQVSDVIT
                     STNASTYTTEYYVVKMVKNQNKGNDMDKYKKELKEIATDTKLSDSTFQNKVIGEVLKD
                     ANVKIKDKDFENVLSTFTSDSSTASSK"
BASE COUNT          536 a          197 c          259 g          350 t
ORIGIN      
        1 tggtagtttt aatatattca tttttagttg tataaaaata tatcataata aaaacaacaa
       61 aaaaatgtct ggaaaatcga acataaaagt gtctgatttg aatatcccag cagcggaaga
      121 agaaaaacaa aatcgataac atttgttcta agaattagtt acgttaaata cggaacgcaa
      181 gatagacttg aaaaacggta acaaaagaag tagtatatta aggtgtgatg tgctacttga
      241 catttattct gctgagcgaa ctttctggat tgtgtagcct tatatctatt caggacgtat
      301 cgagcagatt agtaaaggtg ttcttgatga ttttcatctt gaacaccttt gtttttagca
      361 attcgcaaaa aacgaaaagt aggagaattg ataaatgaaa aaaagatttt tagcacttgc
      421 catcgtacta ggcactggct tattgtcagg ctgtacgaat gccggtgaaa aaacagccgt
      481 tagttataaa ggcggtacaa tcagcgaaca agaagtaatg gatagcttga aaaaaatgca
      541 aggagcagat tccgctgtgc aacagttgat cgtttatcaa gtatttgaag ataaatatgg
      601 agatgacgta tcaaccaaag aaatcgattc tcaatatgat caaacaaaga aacaattggg
      661 tgattcattt gattcacaat tgaaatctgc aggttacaca gaacaaacat tcaaagacag
      721 tatcaaacaa agtctagcct ttcaagaagg tctaaagaaa catatcaaat tgaccgatga
      781 agatttaaaa acggcttggg aatctttcca tccagaagtg gaagctcaaa ttatccaagt
      841 agcaagtgaa gatgatgcaa aagatgtgaa gaaagctgct gataagggag acgatttctc
      901 taaattagcc aaagacaagt caactgatac tacgacaaaa gaagatggcg gaaaagtgaa
      961 atttgattcc actactacaa cggttccggc agaagtcaaa gaagcagcct ttaagttgaa
     1021 agatggtcaa gtttctgatg tgatcacttc taccaatgct tcaacttata ctacggaata
     1081 ttatgtggtt aaaatggtga aaaaccaaaa caaaggcaac gacatggata aatacaaaaa
     1141 agaattgaaa gaaattgcaa cagacacaaa attaagtgac agtaccttcc agaataaagt
     1201 aattggcgaa gtattgaaag acgctaatgt aaaaatcaaa gacaaagact ttgaaaatgt
     1261 tttgagtaca ttcacttcag attctagtac agcaagtagt aaataagcgg tataaataaa
     1321 aaatatgaac tatttaaatc aa
//
LOCUS       DACDKM010000237         1322 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20834_2016
            SAMN12640370-rid9891663.denovo.243, whole genome shotgun sequence.
ACCESSION   DACDKM010000237 DACDKM010000000
VERSION     DACDKM010000237.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640370
            Sequence Read Archive: SRR10040891
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1322)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1322)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742835.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742835.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:04:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,012
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 29 of 75
            Pseudo Genes (incomplete)         :: 33 of 75
            Pseudo Genes (internal stop)      :: 19 of 75
            Pseudo Genes (multiple problems)  :: 5 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1322
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640370-rid9891663.denovo.243"
                     /isolate="E20834_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..573
                     /locus_tag="IV334_002865"
     CDS             <1..573
                     /locus_tag="IV334_002865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8944865.1"
                     /translation="LDIAKRNTAFLLYRVLLRAGLITFISIVGFFLFTVIVFIWRTTA
                     PPLPEITYGEFPFSLEYEIDEERILIEDTVIAEFIGSFRGNVTTQPRRSWSIKLLNNS
                     GELKAFHYEFLIIESEDVTITLSSGSAGYFMNDGGSKPQLTVRDRIAGFMHFSHYDLE
                     EARKILAEYGITLINWEYAPPIRNFFNDYY"
     gene            760..>1322
                     /locus_tag="IV334_002866"
     CDS             760..>1322
                     /locus_tag="IV334_002866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF443 family protein"
                     /protein_id="HAP8944866.1"
                     /translation="METVRLWIFMKNFRSALRYVILESKSGYYLIDTDHYTFWGGYLF
                     PIFNWIIPHKVMKINLPAEEINRLILNKDLQRKREKEHSNVAWIGPAFAVVTSSIVGP
                     MLESIYSDFSIGIRYLFVTLSIAVLLCIKGYVSKKNKDILTLIGVNNLSSTKIILFPE
                     SIKEGSRLLIIFIMLILFSVLFGVMFI"
BASE COUNT          430 a          161 c          264 g          467 t
ORIGIN      
        1 ctagatatag caaagcgaaa tacagcattt ttattatatc gggttttact tcgagcaggt
       61 ctcataacat ttatatcgat cgttggattt tttctattca cagttattgt atttatttgg
      121 agaactacag caccaccatt gccagagatt acatatggag aatttccgtt tagtcttgaa
      181 tatgaaatag atgaggaaag aattttgatt gaagatacgg tgattgcaga atttatagga
      241 agttttagag ggaatgtgac aactcaacct agacgaagtt ggagtataaa acttttaaat
      301 aatagtggag agttgaaagc atttcattat gaattcttaa taatagaaag tgaagatgta
      361 acaatcactt taagtagtgg ctctgctgga tattttatga atgatggtgg aagcaagcca
      421 cagctcacag tccgtgatcg tattgcaggt tttatgcatt tttctcacta tgacttagaa
      481 gaagctcgta aaatacttgc agaatatggg atcacattaa tcaattggga atatgcgcca
      541 ccgataagaa attttttcaa tgattattat tagtcttata aaaaacagaa tgtaaaattt
      601 atgtgcgcga gtatttagtt ttccattttt tgtttgatag atatatagca ttgttgaagc
      661 atgggatagt ggtgccaatt ttatcaatga ggttggtcag gcagttaaca taattggtga
      721 tgctataaat ccatttaatt ggaattggag gaattaggtg tggaaacagt gagattatgg
      781 atattcatga aaaattttcg ttcggctttg agatatgtta ttctggaatc aaaatcaggt
      841 tattatctga ttgacacaga tcactatact ttttggggag gctacctttt tccgattttt
      901 aattggataa tcccccataa ggttatgaaa attaacttgc ctgcagaaga aataaataga
      961 ttaatactca acaaagattt acaaaggaaa agagagaaag aacattctaa tgttgcttgg
     1021 ataggtccag catttgcagt agtgacttct tcaattgtcg gtcctatgct agagagtatt
     1081 tatagtgatt tttctattgg aatacgttat ttatttgtta ctttaagtat agctgttctt
     1141 ttgtgcataa agggttatgt tagcaagaag aataaagata ttttaacact aatcggtgta
     1201 aacaatttat ctagtactaa aattatactg tttcctgaat ctataaaaga aggatcaagg
     1261 ttgttgataa tttttataat gcttatactg ttttctgttt tatttggggt gatgtttata
     1321 ga
//
LOCUS       DACDKM010000238         1318 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20834_2016
            SAMN12640370-rid9891663.denovo.244, whole genome shotgun sequence.
ACCESSION   DACDKM010000238 DACDKM010000000
VERSION     DACDKM010000238.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640370
            Sequence Read Archive: SRR10040891
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1318)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1318)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742835.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742835.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:04:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,012
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 29 of 75
            Pseudo Genes (incomplete)         :: 33 of 75
            Pseudo Genes (internal stop)      :: 19 of 75
            Pseudo Genes (multiple problems)  :: 5 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1318
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640370-rid9891663.denovo.244"
                     /isolate="E20834_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            188..391
                     /locus_tag="IV334_002867"
     CDS             188..391
                     /locus_tag="IV334_002867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8944867.1"
                     /translation="MPEFVNTPNFYQDSDILSKINLPKEESALQVQRTLQETQKIQKS
                     ISNLEDSEEKRKKRDQKTYQKEK"
     gene            602..1186
                     /locus_tag="IV334_002868"
     CDS             602..1186
                     /locus_tag="IV334_002868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAP8944868.1"
                     /translation="MKIGYARVSTGLQNLDLQEDSLNKFGCEKIFTDHMSGAKSNRPG
                     LEMAIDFVRSGDTLVVWRLDRLGRNMEDLISIVNRLNERGVSFHSIQENITMDKSSST
                     GQLMFHLFAAFAEFERNLILERSAAGRAAARARGRFGGRPEKLSEQDLELLKTLVDSG
                     TPIKTIAERWNVSRTTIYRYLAKMEEKNGKTNSH"
BASE COUNT          465 a          183 c          252 g          418 t
ORIGIN      
        1 agtgacagga acgatgattt ttgataaagt tggttggcct tctagacctc ctcaatcacg
       61 gaattggaaa gaaaaaagaa atggttattg gtactatgga aaattgtatt tacaaagtta
      121 taaatcagct ggaaacaatt ggattgcgac ttattcagga accttaacta gaaagtaggg
      181 attttttatg ccagaatttg tgaacacacc aaatttttat caagatagcg atattctaag
      241 taaaattaat ttaccgaaag aagaatcagc tttacaagtt cagcgaactt tacaggagac
      301 acaaaaaatt caaaaatcaa tttctaatct agaagattct gaagaaaaaa gaaaaaaaag
      361 agaccaaaaa acatatcaaa aagaaaaata atgattttaa aaaaagagtg tctagtcgac
      421 attctttttt tctaattgaa agtgttcata aaatcaccca tttctggaca cttccttttt
      481 ttgctttatt tttcaaaaaa acaattcatt tcatgttcat aaacccataa caatatctat
      541 gttcagttaa actatatgga taaagttatg ttacaataaa attaatgaag gagtgatttt
      601 tttgaaaata ggatacgccc gggtatctac tggtttacaa aatttggatt tacaagaaga
      661 cagcctaaat aaatttggtt gtgaaaaaat ttttactgat catatgagtg gggcaaaaag
      721 taatcgacca ggattagaaa tggccattga ttttgttcgc tctggcgata cacttgtcgt
      781 ttggcgctta gatcgtttag gtagaaatat ggaagatttg atttcaatcg tcaatcgact
      841 gaatgagcgt ggtgtgagtt ttcatagtat tcaagaaaat attacgatgg acaaatcaag
      901 ttctacagga cagctcatgt ttcatttgtt tgcggcattt gcagaatttg agcgtaattt
      961 gatcctggaa cgatccgcag caggacgagc agctgctcgt gcaagaggac ggtttggtgg
     1021 tcgtcctgaa aaattgtctg aacaagattt ggaattactg aagactttag tggatagcgg
     1081 tacacctatt aagactattg cggaacggtg gaacgtctca agaaccacca tttatcgtta
     1141 tttggcaaaa atggaggaga aaaatggaaa gacaaattct cattagtttt tgtcatttaa
     1201 tgctgaaaat ttttgccatt tatctagaaa agtgacaata gtatgagaat tttttgtaag
     1261 tgtctactga taacttattc tcaaatgttg taaggatttt attcatttgt taatatta
//
LOCUS       DACDKM010000239         1311 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20834_2016
            SAMN12640370-rid9891663.denovo.245, whole genome shotgun sequence.
ACCESSION   DACDKM010000239 DACDKM010000000
VERSION     DACDKM010000239.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640370
            Sequence Read Archive: SRR10040891
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1311)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1311)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742835.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742835.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:04:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,012
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 29 of 75
            Pseudo Genes (incomplete)         :: 33 of 75
            Pseudo Genes (internal stop)      :: 19 of 75
            Pseudo Genes (multiple problems)  :: 5 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1311
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640370-rid9891663.denovo.245"
                     /isolate="E20834_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            68..253
                     /locus_tag="IV334_002869"
     CDS             68..253
                     /locus_tag="IV334_002869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP8944869.1"
                     /translation="MSENLDLKIRAEMRKRRMTFKELAALIGISGAYLSDILNGNRDG
                     KKAQQHIETVKKILDIR"
     gene            265..513
                     /locus_tag="IV334_002870"
     CDS             265..513
                     /locus_tag="IV334_002870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8944870.1"
                     /translation="MTKLKKQDFVKKYNYSPSTYQRRMSELKNTAIFSAAYERVTGQE
                     VWINTELYDKFLSFKSYNRLRTRKVTPKEFIEKHLVDL"
     gene            544..723
                     /locus_tag="IV334_002871"
     CDS             544..723
                     /locus_tag="IV334_002871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8944871.1"
                     /translation="MGKFNRALVFSAPLIIYALGLWGSRQALIGTIVYMVWIFMGLDE
                     AEAEYRAKKPTEGAD"
     gene            complement(766..1236)
                     /locus_tag="IV334_002872"
     CDS             complement(766..1236)
                     /locus_tag="IV334_002872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8944872.1"
                     /translation="MSIISFILSTFLTGIKIKGMRPQLEVELNASYFAADMIYTKVII
                     SNYSTEPAMLVNLELSSANLNRRWSATPFKKLIAKGGSVDDNRIYSEAVPLNIPPKSA
                     ISCYLAFEVGKINFNKLLNHQTKMIFTLNRTQIHKIVDIKNTNFPIEKLVKELN"
BASE COUNT          466 a          185 c          248 g          412 t
ORIGIN      
        1 ttattgtcaa cgaatattag ctaatttttt agcttacaga ttattttttt agaaaggagc
       61 tatttttatg tctgagaatt tagacttaaa aattcgagcg gagatgagaa aaagaagaat
      121 gactttcaaa gaactagctg cgcttatcgg tatttcagga gcttatttat cagatattct
      181 aaacggcaat cgtgatggaa agaaagcaca acagcatatc gaaacagtga aaaaaatatt
      241 ggacatccga taggggggta ggcgatgaca aaactaaaaa aacaagattt tgtaaaaaaa
      301 tacaattatt ctccatctac ttatcaacgt cgaatgtcgg aactaaaaaa tacagcaatt
      361 ttctcagcgg cgtatgaacg ggtcacagga caagaagttt ggatcaatac agaattatac
      421 gataaatttt tgtctttcaa atcctataac aggttacgca caagaaaggt aacacctaaa
      481 gaatttatcg agaagcattt agttgattta taaaaaacag aacattttga gaggtgaagg
      541 ttaatgggta aattcaacag agcattggta ttcagcgcac cgctaatcat ctacgcttta
      601 ggactttggg gaagcaggca agcgttgata ggaacgattg tttacatggt ttggattttt
      661 atggggcttg atgaagctga agctgagtac agagcgaaaa agccaaccga gggggctgac
      721 taaaaatgaa tagaaaagag aaactagaat gtatattact attactcagt ttaattcttt
      781 cactaatttc tctattggga agtttgtatt tttgatatca acaattttgt ggatttgggt
      841 tctatttaga gtaaatatca tttttgtttg gtgattaagg agtttattaa aattaatttt
      901 ccctacttca aatgcaaggt aacaagatat agcactttta ggaggaatat ttagcggtac
      961 agcttcagag tatattctat tatcatcgac tgatcctcct ttagcaatta atttcttaaa
     1021 tggagtagct gaccatctgc ggtttaagtt agcggatgaa agttctaaat taactaacat
     1081 agctggttct gttgaatagt tagaaatgat tacttttgta taaatcatat ctgcagcgaa
     1141 gtaagaagca ttaagttcaa cttctagttg agggcgcatt ccttttattt ttatccctgt
     1201 taaaaacgta ctcaatataa aacttattat tgacatccat tgaaaaattg ttaagtgaaa
     1261 aaaattcaaa ataaacacca ccagttttta actaaattat accaaaaagg a
//
LOCUS       DACDKM010000240         1302 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20834_2016
            SAMN12640370-rid9891663.denovo.246, whole genome shotgun sequence.
ACCESSION   DACDKM010000240 DACDKM010000000
VERSION     DACDKM010000240.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640370
            Sequence Read Archive: SRR10040891
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1302)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1302)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742835.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742835.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:04:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,012
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 29 of 75
            Pseudo Genes (incomplete)         :: 33 of 75
            Pseudo Genes (internal stop)      :: 19 of 75
            Pseudo Genes (multiple problems)  :: 5 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1302
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640370-rid9891663.denovo.246"
                     /isolate="E20834_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            52..>1302
                     /locus_tag="IV334_002873"
     CDS             52..>1302
                     /locus_tag="IV334_002873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HAP8944873.1"
                     /translation="MSHTQLIKDTLNILDLNIYFEENCLTKEKYKGQICLIYKGTLLY
                     KPEECSHCLCVVPSRIIRWGTTTVRLLLNDVSEYRTYLELKKQRFKCKSCQRTFVADT
                     SVAEKHCFISQKVRWSVIARLKENTSMTEIARQKNISTSSVYRVMKRFYRPLNPFKQT
                     LPKVLCFDEFKSVRSVASAMSFIMMDGQSHALLDIVENRRLPYLERYFSRFSLATREA
                     VQLIVIDMYAPYVSLVKKLFPNAQLIIDRFHIVQHIGRTFLNHRVKETTVRLKGNSHS
                     QRGLGKKLKRYWKLLQKEEAKLNYEKRVWRASFKDYLTESEIADRLLAACPNLRQGYQ
                     LYQDILYAVKKRDQSLFEDCLTRDISGLPETYTTTLRTFKKFLPQIQNALHYSYSNGP
                     LECLNNHIKVLKRNAYGFRNFYNFK"
BASE COUNT          403 a          260 c          252 g          387 t
ORIGIN      
        1 cctgaaatcc tttagaatta atttggctac caattcatag gaggattcaa gatgtctcat
       61 actcagctta tcaaagatac cctaaatatt ctagacctaa atatctattt cgaggaaaat
      121 tgtttaacta aagaaaaata caaagggcaa atttgcttga tttacaaagg aactcttctg
      181 tacaagccag aagagtgttc ccattgtctt tgtgtcgtcc cctctaggat cattcgttgg
      241 gggacgacaa ccgttcggtt gttactgaat gatgtgtctg agtatcgcac ttatctggag
      301 ctgaagaaac aacgatttaa atgtaagtct tgccaaagaa cattcgtagc ggatacctct
      361 gttgctgaaa aacactgttt cattagtcaa aaagttcgtt ggtctgtcat tgctcgctta
      421 aaagagaata cttccatgac ggaaatcgct cgtcagaaaa atatttccac ttcttcggtc
      481 tatcgtgtca tgaagcgatt ctaccgccca ttgaatcctt tcaaacagac tttgccaaag
      541 gtcttatgct ttgatgaatt caaatcggtt cgaagtgttg ccagtgcaat gagttttatc
      601 atgatggacg gacaaagcca tgcattattg gatattgtcg aaaatcgacg cttgccttat
      661 ttagaacgct atttttctcg gttttcatta gcgactcgtg aagccgttca attgattgtt
      721 attgatatgt atgcgcctta tgtttcgttg gtgaaaaagc ttttcccgaa tgctcagttg
      781 atcatcgatc gtttccacat cgtccaacat attggcagaa ctttcttgaa tcatcgagtg
      841 aaagaaacca cggtacgact taaaggaaat tcccactctc aacgagggtt agggaaaaaa
      901 ttgaagcgct attggaaact cctacaaaaa gaagaagcaa agttaaacta tgaaaaacgg
      961 gtttggcgtg ccagttttaa agattatttg actgaaagtg agattgccga tcgactgtta
     1021 gctgcttgtc ccaacttacg ccaaggctat cagttatacc aagacattct ttatgcggta
     1081 aagaaaagag atcagtcact atttgaggat tgtttaacta gagacattag cggtctgcca
     1141 gaaacctata caaccacttt acgaacgttt aaaaagtttc tgccacagat ccaaaatgcg
     1201 cttcactaca gctattcaaa cggaccattg gaatgcttaa acaaccacat caaagtatta
     1261 aaacgcaatg cgtacggctt tcgcaacttt tacaatttca aa
//
LOCUS       DACDKM010000241         1277 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20834_2016
            SAMN12640370-rid9891663.denovo.247, whole genome shotgun sequence.
ACCESSION   DACDKM010000241 DACDKM010000000
VERSION     DACDKM010000241.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640370
            Sequence Read Archive: SRR10040891
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1277)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1277)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742835.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742835.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:04:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,012
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 29 of 75
            Pseudo Genes (incomplete)         :: 33 of 75
            Pseudo Genes (internal stop)      :: 19 of 75
            Pseudo Genes (multiple problems)  :: 5 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1277
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640370-rid9891663.denovo.247"
                     /isolate="E20834_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..118
                     /locus_tag="IV334_002874"
     CDS             <1..118
                     /locus_tag="IV334_002874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HAP8944874.1"
                     /translation="LSLDPRLGVFTLESPSGSLDSHVQLVDLRPEKFSGIKQ"
     gene            complement(232..861)
                     /locus_tag="IV334_002875"
     CDS             complement(232..861)
                     /locus_tag="IV334_002875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HAP8944875.1"
                     /translation="MKIVFLHGLGQDEQSWKDVISYLPNSYSCKSLSLFNHLNSTDTA
                     TLDDLTKYIEVQLNQIKEPFILCGLSLGAVIALNYLATPSPYLKKVVLVAPQFESPNR
                     LLLTFQNIYFYCVPSKIFEKLGLSKKQTLTLLRSLETLDCKKQLRTSRLPKLILCGTK
                     DHANKPAAKRLYKLSTNGRLLFIKNGKHELNREKPEIMAQAMRSFIENS"
     gene            complement(995..1067)
                     /locus_tag="IV334_002876"
     tRNA            complement(995..1067)
                     /locus_tag="IV334_002876"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1032..1034),aa:Thr,seq:ggt)
BASE COUNT          442 a          200 c          210 g          425 t
ORIGIN      
        1 cctttcttta gatccacgtc taggtgtatt tactcttgaa agcccaagtg gttctttaga
       61 cagtcatgtt caattagtag atctacgacc agaaaaattc tctggaataa aacaataact
      121 ttctctctat tgtccataaa actagtctca atcaagctgt attgaaaaaa caagacaaca
      181 ttcttaccat ggtaaataaa aagacgacac attttgtcgt ctttttttaa attaactatt
      241 ttcgataaaa gatcgcatag cttgtgccat tatttctggc ttttctctat tcaattcatg
      301 ttttccgttt ttaataaaga gtagccggcc attcgttgaa agtttatata aacgttttgc
      361 tgctggttta ttcgcatgat ctttggtacc gcataaaatc aatttaggaa gtcgtgatgt
      421 acgaagctgt tttttgcagt ctaatgtttc aagtgaacga agtagggtaa gggtttgttt
      481 tttagataga cctaattttt caaatatctt agaaggtacg cagtaaaaat aaatattctg
      541 gaaagtaagc aataagcgat taggagactc aaattgtgga gcgactaaaa caaccttttt
      601 taaatatgga gaaggagttg caagatagtt taaagcaata acagcgccta aagataatcc
      661 acataaaata aaaggttcct ttatctgatt caattgtact tcgatgtatt tggttaagtc
      721 gtctaaagta gcagtgtctg tgctgtttaa atgattgaac aatgaaagac ttttgcaaga
      781 ataactattg ggtagataag agattacatc tttccatgat tgttcgtctt ggcctagacc
      841 atgcaaaaaa acaattttca tattgctctc ctttttatag ttatcatata gactttcttt
      901 gtactattaa aatataaaca ttcatttctt acgtcataaa ataaaaaaac agcgcagaca
      961 cattgttaaa ccaacatgtt gcactgttta aggaagccac ttgccgggct tgaaccggcg
     1021 acctcttcct taccatggaa gcgctctacc agctgagcta aagcggcatt atcattaata
     1081 caaataatag gataacgaat attaatgtat ttgtcaacag aattttttcg aaaaaatgtg
     1141 tttctctaat aggaaaacag attttagaaa aaataaaaat aacaaaaaat gcgaacggtt
     1201 tctttgataa tataataaaa aaattcaaaa aaagtattgc gttatttaga agaaattgat
     1261 attatattca tgtactt
//
LOCUS       DACDKM010000242         1248 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20834_2016
            SAMN12640370-rid9891663.denovo.248, whole genome shotgun sequence.
ACCESSION   DACDKM010000242 DACDKM010000000
VERSION     DACDKM010000242.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640370
            Sequence Read Archive: SRR10040891
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1248)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1248)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742835.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742835.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:04:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,012
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 29 of 75
            Pseudo Genes (incomplete)         :: 33 of 75
            Pseudo Genes (internal stop)      :: 19 of 75
            Pseudo Genes (multiple problems)  :: 5 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1248
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640370-rid9891663.denovo.248"
                     /isolate="E20834_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            82..>1248
                     /locus_tag="IV334_002877"
     CDS             82..>1248
                     /locus_tag="IV334_002877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3-like element ISEfa11 family transposase"
                     /protein_id="HAP8944876.1"
                     /translation="MNDSIKKMLRITEKDLMITEVSYETLQKKKTLVVDAVLSPTPRA
                     CRSCGSTVVDGNGKAIIVKNGKKETIVRFEQYNHMPLVMRLKKQRYTCKNCRTHWTAQ
                     SYFVQPRHSIANHVRYKIASLLTEKVSLSFIAKSCQVSLTTVIRTLKEFKSYLPKQSK
                     KILPRVLMVDEFRSHASIEDKMSFICADGETGKLIDVLPTRKLPRLTSYFLGCTNPEE
                     VEFLVTDMNAAYFQLTKRVLPNAKVVIDRFHIVKHMNQAFNELRIREMNELRKAGQKS
                     QAEKLKKNWRFLLKNRANINHYEYKTWKSFRAPKYPFLTEAMMIDRLLEFSPPLKEAY
                     PFFHELVEAFRDKDPDLFFSLLAELPETLDDSFREKLQNLLTYEEGITNAMIYPY"
BASE COUNT          414 a          245 c          239 g          350 t
ORIGIN      
        1 attttaggta ttaaaaaaca tatcgttctc tagtatgatt aaatcaccac aaaataaaca
       61 actgagagga cgatacgcta tatgaatgat tctatcaaaa aaatgctgag aataacagag
      121 aaagatttga tgattacaga ggtctcttac gagacccttc agaagaaaaa gacgttggtc
      181 gtcgatgctg ttctctcgcc tactcctcgt gcttgtagaa gttgtggttc tactgtggta
      241 gatggaaacg ggaaagcaat tatagtgaaa aatggaaaaa aggaaacgat tgtccgtttt
      301 gaacaataca atcatatgcc tttggttatg cgcctaaaaa agcaacgcta tacctgtaaa
      361 aactgtcgca cccattggac ggctcaaagt tattttgtcc aacccagaca ttcaatcgca
      421 aatcatgtta gatataaaat tgcttcttta ctgactgaaa aagtatcttt atcttttatt
      481 gcgaaaagct gtcaggtatc tttgaccacc gttattcgta cattgaaaga gtttaaaagc
      541 tatttaccaa agcaatctaa gaagatcctc cccagagtat tgatggttga cgaatttcgt
      601 tcgcatgctt ccatagaaga taagatgagc tttatttgcg cggatggcga aacaggaaaa
      661 ttaatagatg ttttgcctac gcgtaaatta cctcgattaa caagctattt cttaggttgt
      721 accaatccag aagaagtaga attcttggtg acagacatga acgccgccta cttccagctc
      781 accaaacgtg ttctgccaaa tgcgaaagtc gtgattgatc ggtttcatat tgtcaaacac
      841 atgaatcaag cgttcaatga gttgcgtatc cgtgaaatga atgaacttcg taaagccgga
      901 cagaaaagcc aggcagaaaa actgaaaaag aactggcgct ttttgctaaa aaatcgtgca
      961 aacatcaacc attatgaata caaaacatgg aaaagtttcc gagcaccaaa atacccattt
     1021 cttactgaag caatgatgat tgatcgattg cttgagtttt ctccgcccct aaaggaggcg
     1081 tatccctttt ttcatgaatt agttgaagcc tttcgagaca aagaccctga cttatttttc
     1141 tccttattgg cagaacttcc cgaaacgtta gatgacagct ttcgggaaaa gcttcaaaac
     1201 cttctgacct atgaagaagg catcaccaac gcaatgatct atccttat
//
LOCUS       DACDKM010000243         1246 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20834_2016
            SAMN12640370-rid9891663.denovo.249, whole genome shotgun sequence.
ACCESSION   DACDKM010000243 DACDKM010000000
VERSION     DACDKM010000243.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640370
            Sequence Read Archive: SRR10040891
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1246)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1246)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742835.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742835.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:04:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,012
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 29 of 75
            Pseudo Genes (incomplete)         :: 33 of 75
            Pseudo Genes (internal stop)      :: 19 of 75
            Pseudo Genes (multiple problems)  :: 5 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1246
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640370-rid9891663.denovo.249"
                     /isolate="E20834_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            200..742
                     /locus_tag="IV334_002878"
     CDS             200..742
                     /locus_tag="IV334_002878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8944877.1"
                     /translation="MLLKVAIKCYCVKNKGGIIYEKIVPRGACLLLALCLSLSAVGCQ
                     KSNENNKIKEGQSISSEEEGMSINANGKNETFKPSDYTLEAKKEYVYEYLGLKFKLSD
                     KFRNYIADKKIAMLDDQSPIDKELKYAILTFEKMTEEQKNAVIEKMGDGYKNWQNELE
                     RIGTIGIFEKNTSEEKNLKL"
     gene            832..>1246
                     /locus_tag="IV334_002879"
     CDS             832..>1246
                     /locus_tag="IV334_002879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA family protein disulfide reductase"
                     /protein_id="HAP8944878.1"
                     /translation="MDELKRTEIQIIDKKERPKNGFVLSEKTDLENTEAFNKESVKDL
                     RKLPTKDINGKDFTSKDFEKYDLTMVNVFATWCTACVKEIPDLVEVQNEMKSKGVNIV
                     GVVTDPVDDNGENKEAIEKSKLIHEKTKASYPFLMP"
BASE COUNT          514 a          141 c          238 g          353 t
ORIGIN      
        1 attgatgaag cagatcccaa agggctgctt catcaatttt ttcgtaagtg tccgtacact
       61 tactgtgctt gctcatatat taatcgatca tatactaatc gtgtaagaac aactttaagc
      121 ataatactta tattgttata ccatagtttg gaaaaataaa aaattatatt tcatcaacgt
      181 ttcataaaag gtgtgtagaa tgttattgaa agtagctata aaatgctatt gtgtaaaaaa
      241 taaaggagga ataatttatg aaaaaatagt accaagagga gcatgcctat tacttgcatt
      301 atgtttgagc ttaagcgcag ttgggtgtca aaaaagtaat gaaaacaata aaataaaaga
      361 aggacaatcc ataagttctg aagaggaagg catgtcaatt aatgcaaatg gcaaaaatga
      421 aacttttaag ccatcagatt atacattgga ggcaaagaaa gaatatgttt atgaatattt
      481 agggcttaaa tttaaactct cagacaaatt tagaaattat atagctgata aaaagatagc
      541 gatgttagat gatcaaagtc ctatagataa agaactaaag tatgctattt tgacatttga
      601 gaaaatgaca gaagaacaaa agaatgcagt tatagaaaag atgggcgatg gatataaaaa
      661 ttggcaaaat gagcttgaaa gaattgggac tattggtata tttgaaaaaa atacatcaga
      721 agaaaaaaat ctaaaattat aaaatgtgat actcatacta aaataggagt ttcaagtgat
      781 ggaaaatacg attgctattt aagcacaaat agcggagctg aaagtaatct tctggatgaa
      841 ttaaaaagaa ctgagattca aattatagat aaaaaagaac gtcctaaaaa tggttttgtg
      901 ctatcagaaa aaactgattt agaaaataca gaagcattta acaaagaatc ggttaaagat
      961 ttacgtaaac taccaacaaa ggacataaat ggcaaggatt ttacaagtaa agattttgag
     1021 aagtatgatc ttactatggt aaatgtattt gcaacttggt gtacagcttg tgtaaaggaa
     1081 attcctgatt tggttgaggt tcagaatgaa atgaaaagca agggtgtaaa tatcgttgga
     1141 gttgttacag atcctgttga tgataacggt gaaaacaagg aagcaattga aaagtcaaag
     1201 ttaatacatg aaaagacaaa ggcttcatat cctttcttaa tgccag
//
LOCUS       DACDKM010000244         1245 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20834_2016
            SAMN12640370-rid9891663.denovo.250, whole genome shotgun sequence.
ACCESSION   DACDKM010000244 DACDKM010000000
VERSION     DACDKM010000244.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640370
            Sequence Read Archive: SRR10040891
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1245)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1245)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742835.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742835.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:04:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,012
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 29 of 75
            Pseudo Genes (incomplete)         :: 33 of 75
            Pseudo Genes (internal stop)      :: 19 of 75
            Pseudo Genes (multiple problems)  :: 5 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1245
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640370-rid9891663.denovo.250"
                     /isolate="E20834_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..1244
                     /locus_tag="IV334_002880"
     CDS             <1..1244
                     /locus_tag="IV334_002880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HAP8944879.1"
                     /translation="ITEKEHLLPLEEQFCEHCGSQMKDIGRTKVREEIRFHQAMLDCL
                     THYQHTYCCKSCEKEGLSSFKKAIVPKPLISNSLGSNSLVAETIRMKFGQKVPAYRQE
                     NYWKQTHGLDISRDNITNWHIKAVQNALDPLGERLRVYLNQEEILHGDETSYRVIESA
                     KTDTYYWQFCTGKDSQHPIVYYHHDESRAGDVPKTFLKEFTGYLHCDGYSGYNAVESV
                     RLVYCFAHVRRKFFEAIPKGKKNTDIPAAQAVKQLDKWFVLEKKWKDFSPEKRLSCRQ
                     QELRPLFIAFYEWMATIDPVAKSKLDAAVQYACKLRSGFEPIFEDGRLELTNNRAERN
                     IKELVIGRKNWLHSTSLEGARTSGIILSVYKTAELNGLNPVKYLEFLFDKIPNLPVLS
                     AETLDQLLPWNKDVQQHFSRN"
BASE COUNT          410 a          222 c          268 g          345 t
ORIGIN      
        1 ctataacaga gaaagaacat ctccttcctt tggaagaaca attttgtgaa cattgcggtt
       61 cacagatgaa agatatcgga cggacaaaag tacgagaaga aattcgtttc catcaggcta
      121 tgttggattg tctcacccat tatcaacata cctattgttg taaaagctgc gaaaaagaag
      181 gactttcttc ttttaaaaaa gcgatcgttc ctaaaccttt gatttctaat agcttaggtt
      241 ccaatagttt agtggcggaa acgatccgga tgaaatttgg tcagaaagtt cctgcctatc
      301 ggcaagagaa ctattggaaa caaacacatg ggttggatat ttcgagagat aatatcacca
      361 attggcatat taaagcagtg caaaacgcgt tagacccttt aggagaacga ttaagagtct
      421 atcttaatca agaagagatt ctacacgggg atgaaacaag ttatcgtgtg attgaaagtg
      481 ccaaaacaga tacctattac tggcagtttt gtacaggaaa agacagtcag catcccatcg
      541 tttattatca ccacgacgaa agtcgtgctg gagatgttcc gaaaactttt ctcaaggagt
      601 ttacagggta tttacactgt gacggctata gcgggtataa tgccgtggaa agtgtacgac
      661 ttgtctattg tttcgcgcat gttcggcgga aatttttcga agcaattcca aaaggaaaga
      721 aaaacacgga tattcccgca gctcaagcag tcaaacaatt ggataaatgg tttgtcctag
      781 aaaagaaatg gaaagacttt tctcctgaga agcgattaag ctgtcggcaa caagaactgc
      841 gtccattatt tattgctttt tatgagtgga tggcaacgat agatcctgtt gcaaaatcca
      901 agttggatgc agccgttcaa tatgcgtgta aactacgaag tggttttgaa cctatttttg
      961 aagatggtcg tttagaactc acaaataatc gtgcggagcg aaatatcaaa gagctggtga
     1021 taggacggaa aaattggttg cattccacaa gtcttgaagg agcacgaact tcaggaatca
     1081 ttctgagtgt atataaaaca gcagaactta acggattgaa tcctgtaaag tatctagagt
     1141 ttctatttga taagatacca aatcttcccg tactttcagc agaaacttta gatcaactgt
     1201 tgccttggaa caaggatgta caacaacatt tttctcgtaa ctaaa
//
LOCUS       DACDKM010000245         1214 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20834_2016
            SAMN12640370-rid9891663.denovo.251, whole genome shotgun sequence.
ACCESSION   DACDKM010000245 DACDKM010000000
VERSION     DACDKM010000245.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640370
            Sequence Read Archive: SRR10040891
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1214)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1214)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742835.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742835.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:04:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,012
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 29 of 75
            Pseudo Genes (incomplete)         :: 33 of 75
            Pseudo Genes (internal stop)      :: 19 of 75
            Pseudo Genes (multiple problems)  :: 5 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1214
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640370-rid9891663.denovo.251"
                     /isolate="E20834_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(348..896)
                     /gene="aac(6')"
                     /locus_tag="IV334_002881"
     CDS             complement(348..896)
                     /gene="aac(6')"
                     /locus_tag="IV334_002881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside N-acetyltransferase AAC(6')-Ii"
                     /protein_id="HAP8944880.1"
                     /translation="MIISEFDRNNPVLKDQLSDLLRLTWPEEYGDSSAEEVEEMMNPE
                     RIAVAAVDQDELVGFIGAIPQYGITGWELHPLVVESSRRKNQIGTRLVNYLEKEVASR
                     GGITIYLGTDDLDHGTTLSQTDLYEHTFDKVASIQNLREHPYEFYEKLGYKIVGVLPN
                     ANGWDKPDIWMAKTIIPRPNSQ"
BASE COUNT          384 a          238 c          171 g          421 t
ORIGIN      
        1 aaataatttt cgttatattg tctattattg ccatacaaag ataaaaaaga ataaacaaaa
       61 agaaactgtg acaaaagtct tgtcacagtc ccttatctga ataaacggtg ttcaaaagcc
      121 agtttctcgg tattaagcca aatttatgca aaaatgtaga aagccatttt cacaaaattt
      181 ttcttactac tcgaaactaa gcgacttttt cacaacctct ggtacacggt gttccaaaag
      241 tagcttcttc ggaaataagt tggaatttca caaaaattca agaagtaatt ttcgtaaatt
      301 ccttcttatt tcttgaagct gattacttct gtcacaacct ctttttatta ttgagaattt
      361 ggtcgaggaa taatcgtttt tgccatccaa atatccggtt tgtcccagcc atttgcattt
      421 ggtaagacac ctacgatttt ataacctaat ttttcataga attcatacgg atgttcacga
      481 aggttctgga tggaagccac tttatcaaat gtatgctcat acaggtccgt ttgacttaac
      541 gttgttccat ggtctaaatc atccgtacct aaataaatcg tgattcctcc tctggaagct
      601 acttcttttt ctaagtaatt gactaatcga gtacctattt ggttctttcg tcgggagctt
      661 tctacaacta atggatgcaa ttcccaacct gtgataccgt attgagggat tgcaccaata
      721 aatcctacta actcatcttg gtctaccgct gctaccgcga ttcgttctgg attcatcatt
      781 tcttctactt cttctgccga gctgtctcca tattcttccg gccaagtcag tctcagtaaa
      841 tcagaaagct gatctttcaa tactggatta ttacggtcaa attcactgat tatcatacgt
      901 cttccccctt gttcttgctt attctaacag tccttttgaa aaaacgaaat gttctcgctc
      961 aaaaatcttt agctaccaat cagtatctct attctttcat tatttatctt acttataaaa
     1021 taaaaaaatt atacagaaac actttattca aaataggaaa gatcaggaaa gccatcaatt
     1081 ggaggaaaaa actctgtttg ttcatcatga tatagattat cttttgcttt aaaaaaaacc
     1141 aattatgaat tgacaaataa aacctgtttt catataataa tcttaatttt tagaattttt
     1201 agataattac aaaa
//
LOCUS       DACDKM010000246         1208 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20834_2016
            SAMN12640370-rid9891663.denovo.252, whole genome shotgun sequence.
ACCESSION   DACDKM010000246 DACDKM010000000
VERSION     DACDKM010000246.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640370
            Sequence Read Archive: SRR10040891
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1208)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1208)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742835.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742835.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:04:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,012
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 29 of 75
            Pseudo Genes (incomplete)         :: 33 of 75
            Pseudo Genes (internal stop)      :: 19 of 75
            Pseudo Genes (multiple problems)  :: 5 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1208
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640370-rid9891663.denovo.252"
                     /isolate="E20834_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            26..1195
                     /locus_tag="IV334_002882"
     CDS             26..1195
                     /locus_tag="IV334_002882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element ISEfa13 family transposase"
                     /protein_id="HAP8944881.1"
                     /translation="MNDFTTEIMEILINKGDLDDLFRRHLELAINALLQAELTVFLDY
                     EKYDRAGFNSGNSRNGNYSRSFKTEYGELNLVIPRDRNGDFSQQTLPAYKRTNDSLET
                     TIIQLVQKGITMSEISDLIEKMYGHYYKPQTISNMSKIVSEDVSAFKERTLEAKYSVI
                     FMDATHIPLKRQTVSKEAVYIVIGIRLDGTKEVLGFTIAPTESAYVWKEILQDLKDRG
                     LEEVLLVVTDGLSGIDDSIHSVYPNAQFQQCCVHISRNIAHKVRVSDRQEICSDFKLV
                     YQASSKEEANNQIRFMIDKWKKQYPRVVKLLMNPAILTFYNFPPSIRRTIYSTNLIEG
                     FNKQLKKYTKRKEQFPNEESLERFLVSQFNNYNQKFLCRVHKGFKEIHDTLESMF"
BASE COUNT          441 a          179 c          206 g          382 t
ORIGIN      
        1 cattcacaga aaagaggact tccatatgaa cgattttact acagaaatta tggaaatact
       61 aatcaataaa ggtgatttgg atgatttgtt tcgtcgtcac ctagaattgg ctattaacgc
      121 tttattacaa gctgaattaa cggtatttct tgactatgaa aagtatgata gagctggatt
      181 taattcaggt aattcccgta atgggaatta ttcgcgctct tttaaaacag agtacggtga
      241 attgaattta gtaataccta gagataggaa tggagacttt tctcaacaaa ctttacctgc
      301 ttataaaaga accaatgact ctttagaaac cactattatt cagctagttc aaaaaggaat
      361 tactatgtcc gaaatctctg atctaatcga aaaaatgtat ggtcattatt acaaaccaca
      421 aactatttca aatatgagta aaattgtgtc tgaagatgtt tcggcattta aagaaagaac
      481 tttagaagct aaatactcag tcatttttat ggatgctact catattccct taaaaagaca
      541 aaccgtatca aaagaagctg tctatatagt aattggtatc aggttggatg gaactaaaga
      601 ggttctagga tttactatag ctccaactga atctgcttat gtttggaaag agattcttca
      661 agatctaaaa gaccgtggtt tagaagaggt tttgttagtc gtaacagatg gtttaagtgg
      721 tattgacgat agtattcata gtgtttatcc aaatgctcaa tttcaacaat gttgtgtcca
      781 tatatctaga aatatcgctc ataaggttcg tgttagtgat cgacaagaaa tctgtagcga
      841 tttcaaatta gtttaccaag caagttcaaa agaagaagca aataatcaaa taaggtttat
      901 gatagataaa tggaaaaagc agtatccacg agtggttaaa ttgctcatga atcctgctat
      961 attaaccttt tataatttcc ctccgtcaat cagaagaact atttattcaa ctaatttgat
     1021 tgaaggtttt aacaaacaat taaagaaata taccaaaaga aaagagcaat ttcctaatga
     1081 agaatctctg gagagatttc tagtatctca attcaacaac tataatcaaa agtttctatg
     1141 ccgagttcat aaaggtttta aagaaataca tgatacatta gaatcaatgt tttaacttga
     1201 tcaacaga
//
LOCUS       DACDKM010000247         1206 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20834_2016
            SAMN12640370-rid9891663.denovo.253, whole genome shotgun sequence.
ACCESSION   DACDKM010000247 DACDKM010000000
VERSION     DACDKM010000247.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640370
            Sequence Read Archive: SRR10040891
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1206)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1206)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742835.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742835.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:04:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,012
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 29 of 75
            Pseudo Genes (incomplete)         :: 33 of 75
            Pseudo Genes (internal stop)      :: 19 of 75
            Pseudo Genes (multiple problems)  :: 5 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1206
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640370-rid9891663.denovo.253"
                     /isolate="E20834_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..>1206
                     /locus_tag="IV334_002883"
     CDS             <1..>1206
                     /locus_tag="IV334_002883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000181735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HAP8944882.1"
                     /translation="VNDLNLEQKDVVESEDNLYVNACPGSGKTRVLTRKIAYQAIKHD
                     GSLKRIIAITYTNRAAEEIKERLSLLSIDDDINIWVGTIHQFCLEFIIYPFKMNLSRV
                     SKGFTIIDDYTQRAYVSQINNMLNLNLKTYELNKIDLSLSHDFKINEVQYTQVSEMYH
                     TMLEKNKEIDFDMILLVAYKILTVNSNVARNIASNIRAIYVDEFQDTRELQYNVIAKL
                     LQNNPQIQSMFVGDIDQAIYGSLDGIAKSVEELEMLTSHTFQSKTLHGCYRSNQRLVD
                     FYSNFQSCPYEIESRGNNKNDRGFISYDCKVTKEDLPNIIKNIIKEKIESGIHEKDIC
                     VLAPQHYPLFSLGEYLKKELPYCNFDAINISPIKVNNHSLFFKLAILYFTESGEKVRR
                     RKLIANDIL"
BASE COUNT          458 a          158 c          207 g          383 t
ORIGIN      
        1 cgtaaatgat ttaaatttag aacaaaaaga tgttgtggaa agtgaagata acctttatgt
       61 aaatgcatgt cctgggagtg ggaaaacaag ggtacttaca aggaaaattg cttaccaagc
      121 aattaaacac gatggatcac taaaaagaat aattgctatc acatacacta atagagctgc
      181 tgaagaaatt aaggaacgcc taagtttact tagtatagat gatgatatta atatttgggt
      241 ggggacaata catcagtttt gtcttgaatt tattatttat ccttttaaaa tgaatctctc
      301 aagagtatca aaaggattta caattattga tgattatact caaagagctt acgtcagcca
      361 aattaataat atgctgaatt taaatctcaa aacgtatgaa ctgaacaaaa ttgatttgtc
      421 actttctcat gattttaaaa ttaatgaagt tcagtatact caagtttctg aaatgtatca
      481 cactatgttg gaaaaaaata aagaaattga ttttgatatg attttactag tagcgtataa
      541 aattttaact gtaaacagta acgttgcacg taatattgct agtaatataa gggcgattta
      601 tgtggatgaa tttcaagata ctagagaatt acaatacaat gtaatagcta aattacttca
      661 aaacaaccct caaattcagt caatgtttgt tggagatatt gaccaagcaa tttatggatc
      721 attagatggt atagctaaat cagttgaaga actagaaatg ctaactagtc acacttttca
      781 atcaaagacg ctacatggct gttacaggtc taatcagaga ttagtagact tctactctaa
      841 ttttcagagt tgtccttacg agattgaaag cagagggaat aataaaaacg accgtggttt
      901 tataagttat gattgcaaag ttactaagga ggatttaccc aacataataa aaaatataat
      961 aaaggaaaag attgaaagcg gcattcatga aaaagatatt tgtgtacttg ctcctcaaca
     1021 ctatcctctg ttttcactgg gtgagtattt aaagaaagaa ttaccgtatt gcaattttga
     1081 tgctataaat atatcaccaa ttaaggtaaa taatcatagt ttatttttca agttagcaat
     1141 tttgtacttt actgaatcag gtgaaaaggt tcgaagaaga aagctaattg caaacgatat
     1201 tttaat
//
LOCUS       DACDKM010000248         1196 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20834_2016
            SAMN12640370-rid9891663.denovo.254, whole genome shotgun sequence.
ACCESSION   DACDKM010000248 DACDKM010000000
VERSION     DACDKM010000248.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640370
            Sequence Read Archive: SRR10040891
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1196)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1196)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742835.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742835.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:04:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,012
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 29 of 75
            Pseudo Genes (incomplete)         :: 33 of 75
            Pseudo Genes (internal stop)      :: 19 of 75
            Pseudo Genes (multiple problems)  :: 5 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1196
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640370-rid9891663.denovo.254"
                     /isolate="E20834_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            124..459
                     /locus_tag="IV334_002884"
                     /pseudo
     CDS             124..459
                     /locus_tag="IV334_002884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997693.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
     gene            555..1196
                     /locus_tag="IV334_002885"
                     /pseudo
     CDS             555..1196
                     /locus_tag="IV334_002885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          454 a          219 c          229 g          294 t
ORIGIN      
        1 attttatata ataaaatgca atacttttcg agagtgtaaa atattttgtg taaatagaaa
       61 aaaggaagtc ccttctgtag aatagagtta ccacaacaca ttcacagaaa agaggacttc
      121 catatgaacg attttactac agaaattcta aagactctag cgaacaaagg cgatttgaat
      181 gaattattcc gtgtccattt ggaaaaagct gtcaatacgc ttctcaaaac ggagttaacg
      241 gctttcctcg attacgaaaa gtacgatcgc attggtttta acacgggtaa ttctcgtaac
      301 ggctcctatg accgtacggt caagaccgag tacggggaac ttcatctcca gattccgcgc
      361 gaccgcaacg gcgagttcaa gcaacagact gttcctgctt atagacggac gaatgatacc
      421 ttagaggaga ccgtcattca cctcttccga aaaggtatgg ataggctaca attaactagt
      481 ggataggcta caattaacta gacagaaaaa ttaaggtgtg tagactagaa gaaaacatac
      541 caggaggaat ttttatgtct aagagaacac gaagaacttt ttcacaagaa ttcaagcaac
      601 aaatcgtcaa tctttactta gctggaaagc cacgtgtaga aatcattcga gaatatgaac
      661 taacagcttc agcatttgac aaatgggtaa agcaatctaa aacgagtggt tcattcaaag
      721 aaaaagataa tcttacgcct gaacaaaaag aattgttaga actacgtaaa agaaaccagc
      781 aattagaaat ggaaaatgat attttaaagc aagcagcgct gatattcgga cgaagagaca
      841 agtaatcgat gcgaataagc atctttaccc tatatcagcg atgtgcagaa tattaggtct
      901 atcacgtcag tcctattatt atcaatcaaa accaaagaaa gacgaatcag aacttgaaga
      961 agtagtcgct gaagaattta tccgcagccg aaaggcctac ggctcaagaa aaataaaaaa
     1021 agccttatca aaacgaggca ttcagatcag ccgacgaaaa attagtagaa tcatgaaaaa
     1081 tagaggatta aaatcgagct atactgttgc ttattttaaa gtacatcatt ctacttgcaa
     1141 tgaagccaaa acgacaaacg tattgaatcg taaattctta agagacaacc cattag
//
LOCUS       DACDKM010000249         1193 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20834_2016
            SAMN12640370-rid9891663.denovo.255, whole genome shotgun sequence.
ACCESSION   DACDKM010000249 DACDKM010000000
VERSION     DACDKM010000249.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640370
            Sequence Read Archive: SRR10040891
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1193)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1193)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742835.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742835.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:04:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,012
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 29 of 75
            Pseudo Genes (incomplete)         :: 33 of 75
            Pseudo Genes (internal stop)      :: 19 of 75
            Pseudo Genes (multiple problems)  :: 5 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1193
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640370-rid9891663.denovo.255"
                     /isolate="E20834_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..1076
                     /locus_tag="IV334_002886"
     CDS             <1..1076
                     /locus_tag="IV334_002886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HAP8944883.1"
                     /translation="VIGMESTSMYSFHPSMFFHEDEKLKKLNTLVTIENPFRVKQFSR
                     MFDENKTDRNDALRIADFLRIQRFTTSPIKEEKYMALQRLTRTRYQLIKQLIRTKQHF
                     LENLTYKCNTLAREMRDESTSLFSATLISLMTEDFTLDELAELPLEAFCDLLQEKGKG
                     RFKQPEKIAKAIQRAITMSYRLGALAQESINVVLSVLVREIRALEKNIKELDKAIEQV
                     IVVIPEYQCLTSIPGVGKVYAAGLIAEIGQIERFEDQTKLAKYAGLSWKVNQSGNYQS
                     QNTPLTKQGNRYFRYYLVEAANSVKNYLPEYKAFYQSKYKEVPKHQHKRALVLTARKF
                     TRLVDTLLRKHQLYTPPRSVIEK"
BASE COUNT          418 a          234 c          210 g          331 t
ORIGIN      
        1 ttgtgattgg catggagtca acgtccatgt acagcttcca tccttccatg ttttttcatg
       61 aagatgagaa actaaagaag ctcaacacac ttgtaacgat tgaaaatcct tttcgggtga
      121 aacaatttag tcgcatgttt gatgagaata aaactgatcg aaacgatgca ttaagaatcg
      181 ctgatttctt gcgtattcaa cgattcacaa cttcccctat caaagaagag aaatacatgg
      241 ccttacagcg tttaacacgt actcgctatc aacttattaa gcaattgatt cgtacaaagc
      301 aacattttct tgaaaatcta acgtacaaat gtaatacgct tgctcgagaa atgcgagacg
      361 aatcgacgag tctttttagt gcaaccctca tttcactcat gacggaagat ttcacattgg
      421 atgaacttgc tgagcttcct ttagaagctt tctgtgattt acttcaagaa aaaggaaaag
      481 gccgtttcaa gcaaccagaa aaaatcgcta aagcaattca acgtgcaatt accatgagtt
      541 accgcctagg tgctcttgca caagaatcaa ttaatgttgt tttaagtgtt ctcgtacgag
      601 aaatacgagc acttgaaaaa aatatcaaag aattagataa agccatcgaa caagtaattg
      661 ttgtcattcc agaatatcaa tgcttaacca gtattccagg cgttggtaaa gtctatgctg
      721 ccggtttaat cgctgaaatt ggtcaaatcg aaagatttga agatcagaca aaattggcaa
      781 aatacgctgg attaagttgg aaagtgaacc aatctggaaa ctatcagtcg caaaatacac
      841 ctctgacaaa acaaggaaat cgatatttta gatactactt agttgaagct gccaactctg
      901 taaaaaatta tcttcctgaa tacaaagctt tttatcaaag taaatataag gaggttccaa
      961 agcatcaaca caaacgtgca ctcgtcctaa ccgcaagaaa atttacgcgt ctggtggata
     1021 cgctactacg taaacaccaa ctctatacgc caccaaggag tgtgatagag aaataacaat
     1081 ccgttattga cgcaaatcct tgaatcaacc cgaaaaaaat ttgattttga tcgggtctag
     1141 tttcgtgcgc ttcaaaacat attactcaat aaagaaaatc ttaaatttca tct
//
LOCUS       DACDKM010000250         1178 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20834_2016
            SAMN12640370-rid9891663.denovo.256, whole genome shotgun sequence.
ACCESSION   DACDKM010000250 DACDKM010000000
VERSION     DACDKM010000250.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640370
            Sequence Read Archive: SRR10040891
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1178)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1178)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742835.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742835.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:04:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,012
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 29 of 75
            Pseudo Genes (incomplete)         :: 33 of 75
            Pseudo Genes (internal stop)      :: 19 of 75
            Pseudo Genes (multiple problems)  :: 5 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1178
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640370-rid9891663.denovo.256"
                     /isolate="E20834_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..>1178
                     /locus_tag="IV334_002887"
     CDS             <1..>1178
                     /locus_tag="IV334_002887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000470930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAP8944884.1"
                     /translation="METIESKISSDNVESLKTIIQQLNETITSVPEIRKIGENINRQL
                     NSIIGSVYSPNLLLSSTMSDDMGSLAKFLSMKPEQNIDLDLLGLGHLNMIYLALKIVE
                     FEACRSRELLNIMLIEEPEAHIHHHIQKTLFEGLNLQKNYTQILMTTHSVHLAEVSEI
                     SRMNVLKSFNGKSIVMNPANNLNSFAKEKLKKNNLNLINAVERYLDVKRNGLLFSKGV
                     ILVEGDAEEILIPQIVKKLFGINLDELGIGLINVGSTSFEYIASLFDDDRIQRKCAIV
                     TDLDEQAIPSEHRLYKSTAQEKGQARKEKLSSLFNENNWVNCFYANTTFEIEFLDVNK
                     ENILTYVSPIIDDAFKDKKTITAYKEEIQSEQYLERNLAMLKLANHVGKGWFAIELGE
                     "
BASE COUNT          452 a          157 c          200 g          369 t
ORIGIN      
        1 tcatggaaac aattgaatca aaaatatcat cagataatgt tgagtcatta aaaacaatta
       61 tacagcaatt aaatgagact attaccagtg ttcctgaaat taggaaaatt ggcgaaaata
      121 ttaatcggca actaaatagc ataattggtt cagtgtattc tcctaatctt cttctttcct
      181 caactatgtc agatgatatg ggttctttag caaaatttct ttcaatgaag ccagaacaaa
      241 atatcgactt agacttacta gggttaggac atctaaatat gatttattta gctttgaaaa
      301 tagtcgaatt tgaagcgtgt cgttcaagag aactacttaa tattatgttg attgaagaac
      361 cagaagctca tattcatcat catattcaaa aaactctgtt cgaaggatta aatcttcaaa
      421 aaaactatac tcagattctc atgaccacac attctgtaca tttagctgaa gtgtctgaaa
      481 tatctcggat gaatgtatta aaatcgttta atggcaaatc tatcgttatg aatccagcaa
      541 ataatctgaa ctcatttgct aaagaaaagt taaaaaagaa caatctaaat ctaataaacg
      601 cagtagagag atatttagat gttaaaagaa atggattgct tttttctaaa ggtgttatct
      661 tagttgaagg agatgctgaa gaaatactta ttcctcaaat agtaaaaaaa ttatttggta
      721 taaacttaga cgaacttgga ataggtttga ttaatgttgg tagtacttcg tttgaatata
      781 tcgctagctt gtttgacgat gatagaatac agagaaagtg tgctattgta actgatttag
      841 atgaacaggc aatcccatca gaacatcgat tgtataaaag cactgcacaa gagaaaggtc
      901 aagcaagaaa agaaaaatta tcctcccttt ttaatgaaaa caattgggtt aattgttttt
      961 atgctaatac aacatttgaa attgagtttt tagatgtgaa taaagaaaat attctaactt
     1021 atgtcagtcc aattatagat gacgcattca aagataaaaa aacaataact gcttataaag
     1081 aagaaataca aagtgaacaa tatttagaaa gaaatttagc gatgttaaaa ctagccaatc
     1141 atgttggtaa ggggtggttt gcaattgagt taggtgaa
//
LOCUS       DACDKM010000251         1165 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20834_2016
            SAMN12640370-rid9891663.denovo.257, whole genome shotgun sequence.
ACCESSION   DACDKM010000251 DACDKM010000000
VERSION     DACDKM010000251.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640370
            Sequence Read Archive: SRR10040891
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1165)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1165)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742835.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742835.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:04:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,012
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 29 of 75
            Pseudo Genes (incomplete)         :: 33 of 75
            Pseudo Genes (internal stop)      :: 19 of 75
            Pseudo Genes (multiple problems)  :: 5 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1165
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640370-rid9891663.denovo.257"
                     /isolate="E20834_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            2..1159
                     /locus_tag="IV334_002888"
     CDS             2..1159
                     /locus_tag="IV334_002888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8944885.1"
                     /translation="MRTIQRKLRELNTILKNYHLSCSLKKGNPIEGEEYRIRYFFHLM
                     YWQSFDENHSDILSSIKNKRQLLEKFAKHAPYTRSIDRKKWISLLRISLQRMKQGHSI
                     TYVPEEMNKFVHPLITKEEFTHYILEPFFEANFWSMNHITPTEQAYLYFMFGVMNTYL
                     EEDLLDTPDKVQESWGWIESSIVENFEKNFKICFTTSEKNYLYVNLAMIHLYSRVFGT
                     KKKVDAFGKSAEDRDFQHIFPVMYPIMQQFFSGVARRIPELNTYYEKNRRLIFQYCML
                     VRDIFIKHEQPVIFYIQSKYGKTQELWAKKRILSMTERPIKYSASADQLPDIVISDYP
                     IDKKAYDQTNTQIFYWNGQPTKNDWARLKAEIIKIRNEKNRSILEKLDFVN"
BASE COUNT          435 a          175 c          214 g          341 t
ORIGIN      
        1 tttgcgtacg attcaaagaa aattaagaga acttaatact attttgaaaa actatcattt
       61 atcttgcagc ttgaaaaaag gtaatccgat agaaggagaa gaataccgta tccgatactt
      121 ttttcacttg atgtattggc agtcattcga cgaaaatcat tctgatatat tatcaagtat
      181 aaaaaataaa cgtcaactat tagaaaaatt tgcgaaacat gcgccatata cgcgtagtat
      241 agatagaaag aaatggatca gcctactaag aatttcgttg caacgaatga aacaaggcca
      301 ctcgattacg tatgtcccag aagagatgaa caaatttgtg catccattga taacaaaaga
      361 agagtttacg cattacatac tagagccttt ttttgaagca aacttctggt cgatgaatca
      421 catcactcca acagagcaag cttaccttta ttttatgttt ggtgtaatga acacctattt
      481 ggaagaagat ttgttggata ctccagataa agtacaagaa agttggggct ggattgaatc
      541 gtctattgta gaaaattttg aaaaaaactt caagatttgt tttacaactt cagaaaaaaa
      601 ttatctatat gtcaatttgg caatgatcca tttgtattca agggtttttg ggacaaaaaa
      661 gaaggtggat gcttttggaa aatcagcaga agacagagat tttcagcata tatttccagt
      721 gatgtatcca atcatgcaac agttttttag tggggtagct cgcagaattc cagagcttaa
      781 tacttattat gaaaagaaca gacggttgat ttttcaatac tgtatgttag tcagggatat
      841 ttttataaaa cacgaacaac ctgtcatatt ctatattcaa tcaaagtatg gtaaaacaca
      901 ggaattatgg gcaaagaaaa ggatattatc tatgactgaa cgcccaataa aatatagtgc
      961 atccgctgat cagttacctg atattgtcat tagcgattat cctatcgaca aaaaggcata
     1021 tgatcaaacg aatacacaga tattttactg gaatggacaa cctacaaaaa atgattgggc
     1081 gcgtctgaaa gctgaaatca taaaaatcag gaatgagaaa aatagatcca tattggaaaa
     1141 attggatttc gttaattaag aaaag
//
LOCUS       DACDKM010000252         1143 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20834_2016
            SAMN12640370-rid9891663.denovo.258, whole genome shotgun sequence.
ACCESSION   DACDKM010000252 DACDKM010000000
VERSION     DACDKM010000252.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640370
            Sequence Read Archive: SRR10040891
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1143)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1143)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742835.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742835.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:04:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,012
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 29 of 75
            Pseudo Genes (incomplete)         :: 33 of 75
            Pseudo Genes (internal stop)      :: 19 of 75
            Pseudo Genes (multiple problems)  :: 5 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1143
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640370-rid9891663.denovo.258"
                     /isolate="E20834_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(18..923)
                     /gene="xerC"
                     /locus_tag="IV334_002889"
     CDS             complement(18..923)
                     /gene="xerC"
                     /locus_tag="IV334_002889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HAP8944886.1"
                     /translation="MTEATWEQKFLRYLIVERGYSEKTKEAYEEDILHFKHFLEESGD
                     ADLLKAEYFTVRVYLSALADHRPSYSRNSISRKIASLRSFYQYLLKEEVIKENPFSYV
                     HLKKKNLRLPRFFYENEMQALFDSVAGDSPLDLRNRALLEVLYGSGIRLSECSSLSVS
                     DIDFDSEVMLIHGKGNKERYAPLGSFAQDALQEYFREGRQVLMTKYHEEHDIVFVNHH
                     GKPITPTGIEYVLNQMIKKSSLDSDIHPHMLRHTFATHLLNNGADLRTVQELLGHANL
                     STTQIYAHVTKESLQKNYRSFHPRA"
BASE COUNT          338 a          232 c          194 g          379 t
ORIGIN      
        1 tgttcttttc cttattttta cgctcttgga tggaaagaac gatagttttt ttgtaaactt
       61 tcttttgtta catgggcata gatctgggta gtcgataaat tggcatgtcc tagcaattct
      121 tggactgtcc gcaaatctgc cccattattt aataaatgag tagcaaatgt atggcgcagc
      181 atgtgaggat ggatatcact atccaaactg ctttttttga tcatttgatt caagacatat
      241 tcaattccag taggcgtgat tggttttcca tgatgattca caaaaacgat atcatgctct
      301 tcgtgatatt ttgtcatcaa tacctggcgg ccttccctga aatattcttg cagagcatct
      361 tgagcaaaag aacctagagg agcatatctc tctttgttcc ctttaccatg gatcaacatg
      421 acctctgaat caaaatcaat gtctgatacc gacaaactgc tacactcact taaacggatg
      481 cctgacccgt aaaggacttc cagtaaagcg cggttcctca aatccaaagg actatctcct
      541 gctacgctgt caaaaagtgc ttgcatttca ttttcataaa aaaaacgtgg cagtctcaaa
      601 tttttctttt tgagatgaac gtaagaaaat ggattttctt ttatcacttc ttctttgaga
      661 agatactgat aaaacgaacg taggctggct atcttgcgac tgatagaatt tctgctatag
      721 ctaggacgat gatcagctaa tgcactcaaa tagacacgaa ccgtaaaata ttccgccttc
      781 agcagatcag cgtctccaga ttcctccaaa aaatgtttga aatggagaat gtcttcttca
      841 tatgcttctt tcgttttttc tgaatatcct ctttcgacaa tcaaataacg caaaaatttt
      901 tgttcccacg tggcttctgt cattttctca ccttcctttt gtcccagaga ataatttagc
      961 acagcgttta taaaaagaca aataaattgt cagaatttaa ccaatatttc taagtttgtt
     1021 cacaattggc gcaattcctc acattttgtt tgagaaattc ttaagaaaaa cgcaagaaaa
     1081 agggctctat aatttatggg actgatgaat cagaattgat tcttcagccc cattttcatt
     1141 tta
//
LOCUS       DACDKM010000253         1121 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20834_2016
            SAMN12640370-rid9891663.denovo.259, whole genome shotgun sequence.
ACCESSION   DACDKM010000253 DACDKM010000000
VERSION     DACDKM010000253.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640370
            Sequence Read Archive: SRR10040891
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1121)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1121)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742835.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742835.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:04:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,012
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 29 of 75
            Pseudo Genes (incomplete)         :: 33 of 75
            Pseudo Genes (internal stop)      :: 19 of 75
            Pseudo Genes (multiple problems)  :: 5 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1121
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640370-rid9891663.denovo.259"
                     /isolate="E20834_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(<1..786)
                     /locus_tag="IV334_002890"
     CDS             complement(<1..786)
                     /locus_tag="IV334_002890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8944887.1"
                     /translation="MATNYAKKINFDVDKLYKSLIVVPKNKSEDGNYELEVLQFKIEQ
                     KSSERFMSNQLVKIKKWLPMIDQLVSNNKEAKNLCIFVLLLHGRVINLVNRKSTTKFA
                     NSFWFLKNINKTVEKNGVYKGDFHFQNAETIEREHHYFDLLVNTVVELAKWAKSNETY
                     SQVLKRIMSQETSFIYELSKSNLKYTYNEDELEILDNPENSQWIKELKREDFIETLRI
                     LKSLFDLKLAKATIMGIERDSYYWEEIGLYAPQIEGEDKEKRID"
BASE COUNT          366 a          204 c          114 g          437 t
ORIGIN      
        1 gtctatcctt ttctctttat cttcaccttc aatctgggga gcatatagtc caatttcctc
       61 ccaataatat gaatctcttt caattcccat gatggtagct ttggctaatt tcaaatcaaa
      121 taacgatttt agaattctta gtgtctcaat aaagtcttct ctcttcaact ctttaatcca
      181 ttgtgaattt tctggattat ctaaaatttc aagttcatct tcattataag tatatttcaa
      241 atttgacttg cttaactcat agataaatga agtttcttga ctcataattc ttttaagtac
      301 ttgactataa gtttcgtttg actttgccca tttcgctaat tctaccactg tattcactaa
      361 taaatcgaaa taatgatgtt ctcgttcaat agtctcagca ttttgaaaat gaaaatcacc
      421 tttataaact ccattctttt ccactgtttt attaatattt tttaaaaacc agaatgagtt
      481 tgcaaattta gtcgtacttt ttcgattaac taagttaata actcttccat gtaataataa
      541 gacaaaaata cataaatttt tagcttcctt attattagat actaattgat caatcatagg
      601 gagccatttt ttgattttca ctaattgatt gctcataaat ctttcactgg acttttgttc
      661 aatcttaaat tgcaaaactt ctaactcata attcccatct tctgatttat ttttgggaac
      721 aactatcaaa cttttataaa gtttatccac atcaaaattt atcttttttg cataatttgt
      781 agccattaat acatctcctt attcataata aatttcatat gacgctcctc tcaaaattta
      841 ccggtagact agaaaaaaga ataaaagata cgtcaattca cactttattc ttaaaaaaaa
      901 actggtctct ctaccggtaa tatcgattga gtccgccttt atcgacttta tccttttttt
      961 atattaaatt ttttcaccgg tagacaggga gagaaagaat aaactaaaaa gcccttaatt
     1021 cttaatgttt tcacctcctt accgttccta ccgctaaaat ttaatataac taaattctat
     1081 aacattagaa gaaattttaa atacctttga taaaaaatat t
//
LOCUS       DACDKM010000254         1117 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20834_2016
            SAMN12640370-rid9891663.denovo.260, whole genome shotgun sequence.
ACCESSION   DACDKM010000254 DACDKM010000000
VERSION     DACDKM010000254.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640370
            Sequence Read Archive: SRR10040891
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1117)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1117)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742835.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742835.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:04:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,012
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 29 of 75
            Pseudo Genes (incomplete)         :: 33 of 75
            Pseudo Genes (internal stop)      :: 19 of 75
            Pseudo Genes (multiple problems)  :: 5 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1117
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640370-rid9891663.denovo.260"
                     /isolate="E20834_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(1..634)
                     /locus_tag="IV334_002891"
                     /pseudo
     CDS             complement(1..634)
                     /locus_tag="IV334_002891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611665.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
BASE COUNT          351 a          190 c          190 g          386 t
ORIGIN      
        1 aggtaaaaaa taagtctgat tccttgtctc taaaggcttc ggtcagctca tggaaaaaag
       61 tataggcgtc ctttaaaggc gttgaaaatg ccagcaatcg atcaatcatc attgcttcag
      121 tgagtagcgg gtatttaggt gcacgaaaac tgcgccatgt tttgtaatca tagtgattga
      181 ttttttgacg gttttttaga agaaagcgcc agttcttttt cactttctcg gcttcccgtc
      241 gttgccctaa ctggtgtaat cttttcattt cttgtacacg aaaatcattg aacgcctgat
      301 tcaaatgttt gatgatatga aaacaatcaa tgacgacttt cgcatttggt aagacacgtt
      361 ttgtcagttg gaagtaggcg gcgttcatat ctgtcactaa atattccacc gcttcaggat
      421 ttgcacagcg actaaaatag tgtgttaatc gagacaattt tcgtgtaggc aaaatatcaa
      481 tgagttttcc agtctcgcca tccgcacaaa taaagctcat tttatccaca attgaagcgt
      541 gcgaacgaaa ttcatccacc atcaatactt ttggcagggt cctttttgac ggcttcggaa
      601 gatagttttt caactctttc aatgtacgaa tcatatacct aaaataacaa tgggactgaa
      661 gaatcaattt tgattcatca gtcccataaa ttatagagcc tatattttgc gaattgcgct
      721 acggtttctt cttttctttt gtctgtaata tgaatatata gatccataat gatttgaata
      781 gaagagtggc ctaaacgctc ttgcacatct ttaatatttg cactagcttg ctaaaagcaa
      841 gctagcatgt gtgtgtctaa gaccatgaat agtaattcgt ttaagattgt ttttcctgat
      901 gattacatct aaccattttc gagatttaga taattgaagg tgttcatttc tttctggtaa
      961 aaatttaaat atataactac attttctcta ctaactttga aacactactt ttgtaatata
     1021 aaaaatattt tattaataat aaaattaatc acattttata tagtgagtaa tctagtgttt
     1081 taaaaaaata aatgatacta tatgtaatat aaaaaaa
//
LOCUS       DACDKM010000255         1098 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20834_2016
            SAMN12640370-rid9891663.denovo.262, whole genome shotgun sequence.
ACCESSION   DACDKM010000255 DACDKM010000000
VERSION     DACDKM010000255.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640370
            Sequence Read Archive: SRR10040891
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1098)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1098)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742835.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742835.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:04:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,012
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 29 of 75
            Pseudo Genes (incomplete)         :: 33 of 75
            Pseudo Genes (internal stop)      :: 19 of 75
            Pseudo Genes (multiple problems)  :: 5 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1098
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640370-rid9891663.denovo.262"
                     /isolate="E20834_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            1..341
                     /locus_tag="IV334_002892"
                     /pseudo
     CDS             1..341
                     /locus_tag="IV334_002892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311681.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase subunit sigma-54"
     gene            375..>1098
                     /locus_tag="IV334_002893"
     CDS             375..>1098
                     /locus_tag="IV334_002893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase subunit sigma-54"
                     /protein_id="HAP8944888.1"
                     /translation="MPIEGETSDLINLINNFCKSIDTTNGLILLVDMGSLEQMYEKIE
                     SNVIGDLVILNNVSTALAIECGIQVCQKKPISHFYQMDFDSFQVKVQYYKGLSQKKNV
                     IVSCLSGEGISEKVKDILKRYLDNQVEILIFDFNALKKIAKEKDTMIFKNTICVLSTT
                     EFYIQGIDCLNLENLINGNQTLEKLNKYMDSDSCEFCLNELVKLFTIEGASTKLRFLD
                     SKKVFNEIEKVLYLYEKYYDVKI"
BASE COUNT          406 a          127 c          198 g          367 t
ORIGIN      
        1 ataaaatatc accaattatt gattcgacag gtctatgttt attagatgaa ttcttggcgg
       61 atatcagtct atttataatg tttattgatg taattgaaga aacatcaaga aattttttgc
      121 tgtctaagtt cagaaaattt cgtatgaaaa atgataaaca aaaattattg gcaaatgaga
      181 tttggagtag tttagaagtc gaagaaagta aacaagaact tttgttgaaa tggatacttt
      241 ttcttgtaaa gaagttctat gtgaaaatac cagaaaccca ttgtctgata gttatgcacg
      301 gtaaaataac agcttctgcg atcgcaaggg aaacaaataa gttgctgaat acatatgttt
      361 atgaggcttt cgatatgcca attgaaggtg agactagtga cttaattaac ctaatcaaca
      421 atttttgtaa aagtatagat acgacgaatg gactcattct gctagttgat atgggatcat
      481 tggaacaaat gtatgaaaaa attgaatcca atgtaatagg tgatttggta attttaaaca
      541 atgtttctac agctttagcg attgaatgtg ggattcaagt ttgccaaaaa aagccaatta
      601 gtcattttta ccagatggat tttgattcgt ttcaagttaa agttcagtat tataaaggat
      661 tatcacaaaa gaaaaatgta atagtatctt gtctctctgg tgaggggatt tctgaaaaag
      721 ttaaggatat tttaaaacgg tatctcgata atcaagtgga gattttgatt tttgatttta
      781 acgcattaaa gaaaatagca aaggaaaaag atactatgat ttttaagaat acaatttgtg
      841 ttctttcaac cactgaattt tacattcaag gaatcgattg tttaaattta gaaaatctaa
      901 ttaatgggaa tcaaacctta gaaaaattaa ataagtatat ggactcggat agctgtgagt
      961 tctgcttaaa tgaattagtt aaactcttta ctattgaagg cgctagtact aagcttcgct
     1021 ttttagattc caaaaaagta ttcaatgaga tagaaaaagt actatatttg tatgaaaaat
     1081 attacgatgt caaaatcc
//
LOCUS       DACDKM010000256         1091 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20834_2016
            SAMN12640370-rid9891663.denovo.263, whole genome shotgun sequence.
ACCESSION   DACDKM010000256 DACDKM010000000
VERSION     DACDKM010000256.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640370
            Sequence Read Archive: SRR10040891
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1091)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1091)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742835.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742835.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:04:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,012
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 29 of 75
            Pseudo Genes (incomplete)         :: 33 of 75
            Pseudo Genes (internal stop)      :: 19 of 75
            Pseudo Genes (multiple problems)  :: 5 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1091
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640370-rid9891663.denovo.263"
                     /isolate="E20834_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..496
                     /locus_tag="IV334_002894"
     CDS             <1..496
                     /locus_tag="IV334_002894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HAP8944889.1"
                     /translation="LFLVMKIIDAIKIEFFTIFFATSLYIVFRMLPVVMISWLLVSVY
                     TSNELLSSLEKIHLPKKIMLSITVVIRFFPTYRSEIRMIKESLKMRNIRLSFFQPLQY
                     LEFLLVPVLIRATMIAEEMTANAITKGIESPVKRSSYYKTNMSLLDYIILFSVTIFFL
                     SILI"
     gene            498..>1091
                     /locus_tag="IV334_002895"
     CDS             498..>1091
                     /locus_tag="IV334_002895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP8944890.1"
                     /translation="MKELFEFNNVSFSYKDVKEQALVDLNFSIKKGSLTLITGSSGSG
                     KSTLLKLMNGLIPRLYDGKLVGEILFENNSLQSLTQESISKNIGYVSQDPRGQFFTTN
                     TTSELVFSMENFGFTKEHMDKRINELAELLDLSNILDKNIFSLSSGERQKISIGCSLS
                     LNSKLIILDEPSSNLDFKATKKLADLLCDLKNKGFTIV"
BASE COUNT          372 a          147 c          182 g          390 t
ORIGIN      
        1 tctgttttta gtaatgaaaa ttattgatgc tatcaagata gagtttttta ctatattttt
       61 tgcgacatcc ttatatattg tatttcgaat gttgccggtc gtaatgatct catggttgtt
      121 ggttagtgtc tatactagca atgaattatt atcctcttta gaaaaaatac atttaccgaa
      181 aaaaataatg ttaagtatta cagtagtgat cagattcttt ccgacttatc gttctgaaat
      241 aagaatgatt aaagaatctt taaaaatgag aaacattcgc ttgtcttttt ttcagcctct
      301 gcaatatttg gaatttctgt tagttcccgt tcttatacgt gcaactatga ttgctgaaga
      361 aatgaccgct aatgcaataa caaaggggat agaatctcct gtgaaaagaa gttcttacta
      421 taaaacaaac atgagcttat tagactatat catcctgttt tccgttacta tttttttctt
      481 aagtattttg atttgaggtg aaagaattgt ttgaatttaa caatgtttct ttttcatata
      541 aggatgttaa ggaacaagcg cttgttgacc ttaatttttc aataaaaaaa ggcagtctta
      601 cattgattac tggttcttct gggagtggga aatccacact attaaagtta atgaatggac
      661 ttattccaag actttatgac ggaaaattgg ttggggaaat tttatttgaa aataactcgc
      721 tacaatcctt aacacaagaa agtatatcaa aaaatattgg ctacgtaagt caagatccta
      781 gaggacaatt ctttacaaca aatacaacga gtgagttagt attttcgatg gaaaactttg
      841 gatttacaaa agagcatatg gataaaagaa tcaatgaatt agctgaatta ttagatcttt
      901 caaatatatt agataaaaac attttttcgc tatcaagtgg tgaacggcag aaaatttcta
      961 ttggatgtag tttatcgcta aactctaaat taattatatt ggatgaaccg tcttcaaatt
     1021 tggacttcaa agcgactaag aaacttgctg acttactttg tgatttaaaa aataaaggat
     1081 ttactattgt t
//
LOCUS       DACDKM010000257         1071 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20834_2016
            SAMN12640370-rid9891663.denovo.264, whole genome shotgun sequence.
ACCESSION   DACDKM010000257 DACDKM010000000
VERSION     DACDKM010000257.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640370
            Sequence Read Archive: SRR10040891
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1071)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1071)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742835.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742835.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:04:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,012
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 29 of 75
            Pseudo Genes (incomplete)         :: 33 of 75
            Pseudo Genes (internal stop)      :: 19 of 75
            Pseudo Genes (multiple problems)  :: 5 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1071
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640370-rid9891663.denovo.264"
                     /isolate="E20834_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            57..413
                     /locus_tag="IV334_002896"
     CDS             57..413
                     /locus_tag="IV334_002896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HAP8944891.1"
                     /translation="MEKISFETFKRPNGHDEFLEWLETLPKKDSAKLLRTIEETEKNG
                     MLVAQRLKWVKKLDTNLYELRSKVGSNIQRAIYFHVVNGRYVITHGFTKKTEKTPISE
                     LKHAKELREEWFTNDD"
     gene            403..669
                     /locus_tag="IV334_002897"
     CDS             403..669
                     /locus_tag="IV334_002897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP8944892.1"
                     /translation="MMIKKLIEEKQKDKDFAEAYDREGRKLETAVALYHAREEAGLTQ
                     AELAQKAHTTQSTIARIERGDNVSFDKLDDIARALGKKLVVEFA"
     gene            828..>1071
                     /locus_tag="IV334_002898"
     CDS             828..>1071
                     /locus_tag="IV334_002898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4062 domain-containing protein"
                     /protein_id="HAP8944893.1"
                     /translation="MKTINLIKVFIASPGDIAAKRNEVEETIYRWNIENTDNTKVILM
                     PIRWETNAIPTYINNSSGQSVINKQIVENSDILLAIF"
BASE COUNT          442 a          134 c          207 g          288 t
ORIGIN      
        1 ttcgaatttt tatgttatat actataatat aaaatcaata tatgatataa ttgggtatgg
       61 aaaaaatatc ttttgaaaca tttaaaagac ctaatggtca tgatgaattt ttagaatggt
      121 tggaaacatt accaaaaaaa gatagtgcga agcttttacg tacaatagaa gaaacagaaa
      181 agaatgggat gctagtagct caacggttga aatgggtgaa aaaattagac actaatttgt
      241 acgaattaag aagtaaggta ggatcaaaca tccaaagagc tatctatttc catgtggtca
      301 atggaaggta tgtcattacc catggattta ctaaaaaaac ggagaaaaca ccaataagtg
      361 aacttaaaca tgcaaaagaa cttagagagg agtggttcac aaatgatgat taaaaaattg
      421 attgaagaaa agcaaaaaga caaagatttt gcagaagcat atgaccgtga aggaagaaaa
      481 ctcgaaactg cggttgctct ttatcatgca cgggaggaag caggcctaac tcaagcggag
      541 cttgcacaaa aagcacatac aactcaatct actattgctc gaattgagag aggggataac
      601 gtatctttcg ataaacttga tgatatcgct agggcattag gcaaaaagct ggtagtagaa
      661 tttgcttaaa agcgagttga aaatatacct gtaataggct cctaactaat caattagttt
      721 agtgggcctt tttgtactaa atagataaaa gttgtcctat agtgtaaaag tcaataaatt
      781 ttatacaaaa attactttaa agagacataa agagaaaggt acaataaatg aaaaccataa
      841 atctaataaa agtatttata gcatcaccag gtgatatagc agcaaagagg aatgaagtag
      901 aagaaaccat atatagatgg aatatagaaa atacagataa tacaaaagta attttgatgc
      961 caattcgatg ggaaacgaat gcgattccta cttatataaa taatagctct gggcaaagtg
     1021 ttattaataa acaaattgta gaaaatagtg atatattatt agcgattttt g
//
LOCUS       DACDKM010000258         1053 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20834_2016
            SAMN12640370-rid9891663.denovo.266, whole genome shotgun sequence.
ACCESSION   DACDKM010000258 DACDKM010000000
VERSION     DACDKM010000258.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640370
            Sequence Read Archive: SRR10040891
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1053)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1053)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742835.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742835.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:04:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,012
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 29 of 75
            Pseudo Genes (incomplete)         :: 33 of 75
            Pseudo Genes (internal stop)      :: 19 of 75
            Pseudo Genes (multiple problems)  :: 5 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1053
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640370-rid9891663.denovo.266"
                     /isolate="E20834_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..587
                     /locus_tag="IV334_002899"
     CDS             <1..587
                     /locus_tag="IV334_002899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HAP8944894.1"
                     /translation="SITIWEEILLDLQERGLKNVLLFITDGLKGMVGAISRFYPKARF
                     QHCCVHVFRNISHKVRVNNRKEVCDDFKMVYQASSKEVALEARGAFAKKWKTSYPKVV
                     ESILSNDHLLTFYDFPLAIRKSIYSTNLIESFNKQIKKYSHRKEQFQNEESMERFLVS
                     SFDTYNQKFLGRSHKGFQQAEGELEQMLSQPMEN"
BASE COUNT          346 a          185 c          196 g          326 t
ORIGIN      
        1 aatccatcac gatttgggag gaaattttat tagaccttca agagcgcggt ttgaaaaatg
       61 tcctcctgtt catcacggat ggcttaaagg ggatggtagg agcgattagt cggttctatc
      121 ccaaagctcg ttttcaacat tgttgtgtac acgtttttcg taatatcagt cacaaagtgc
      181 gtgtcaataa tcgtaaggaa gtctgtgatg attttaaaat ggtgtatcaa gcctcatcta
      241 aagaggtggc gttagaagca cgtggtgctt ttgcgaaaaa atggaaaacc agctatccaa
      301 aagtggttga atcgattctt tcgaacgatc acttgctcac tttctatgat ttccccttgg
      361 ccatacgcaa gagtatttat tctacgaact tgattgaatc ctttaataag caaatcaaga
      421 aatacagcca ccgcaaggaa cagttccaaa acgaagagtc gatggaacgt ttcttagtct
      481 cgtcttttga tacttacaac caaaaattcc taggtcgcag tcataaaggt ttccaacagg
      541 ccgaaggcga gctcgaacaa atgctgagtc aaccgatgga gaattagaaa caatctacag
      601 tagggaagaa ccatttacac aaaattattg acgctcccaa gctaaaactc ctattaatcg
      661 atctactaaa aataaataaa aaaattcttt tcaattttta tttatggtat gacataaata
      721 aagtaaagtt attcttctag gttaatctta ttaatactct ttctcactag tgttgcatgc
      781 aacactagtg agaaagagta ttaaatatac tgcaaggcaa gacatttggt aattgaagaa
      841 taacaagcaa aaaaatagca gtcaggtctc ttgacaaagt atagttgcta ttcttttttt
      901 aggccctact ttaatgaaat ttttgtttgg agggaacgac ttcattcctt tgattatttc
      961 agttgaattt aatagagaag cctggcgtct attaagaaat acttttcttg atgagcgtca
     1021 atttttttaa cggaaatcta tcctatacta aat
//
LOCUS       DACDKM010000259         1040 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20834_2016
            SAMN12640370-rid9891663.denovo.267, whole genome shotgun sequence.
ACCESSION   DACDKM010000259 DACDKM010000000
VERSION     DACDKM010000259.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640370
            Sequence Read Archive: SRR10040891
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1040)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1040)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742835.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742835.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:04:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,012
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 29 of 75
            Pseudo Genes (incomplete)         :: 33 of 75
            Pseudo Genes (internal stop)      :: 19 of 75
            Pseudo Genes (multiple problems)  :: 5 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1040
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640370-rid9891663.denovo.267"
                     /isolate="E20834_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            221..>1040
                     /locus_tag="IV334_002900"
     CDS             221..>1040
                     /locus_tag="IV334_002900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HAP8944895.1"
                     /translation="MPNFTTEIMETLINKGDLDELFRCHLELAVNSLLQAELTAFLDY
                     EKYDRAGFNSGNSRNGNYSRSFRTEYGELNLVIPRDRNGKFSQQTLPAYKRTNDSLET
                     TIIQLFQKGITMSEISELIEKMYGHHYTPQTISNMTKIVSEDIIAFKERSLESRYSVI
                     FMDATHIPLKRQTVSKEAVYIVIGIRLDGTKEVLGFSLAPTESAYVWKEILQDLKDRG
                     LKEVLLVVTDGLSGINDSIHSVYPNAQFQQCCVHISRNIAHKVRVSDRQEICSDF"
BASE COUNT          368 a          150 c          178 g          344 t
ORIGIN      
        1 gttttatagc aaagaaagtt taataagagt ttggacctag caaacaatat taatgaggtc
       61 attaaatctt attatcttta gtgatataac gaaatatcta cacagacaaa atgatagcgt
      121 aaaatatttt gtgtaaatag atatttaggg tagaaaaaaa gaaagtccat tctgtaaaat
      181 taaagttacc acaaccgaaa ttttatagga ggactttctc atgcccaatt ttactacaga
      241 aattatggaa acactaatca ataaaggtga tttagatgaa ttatttcgat gtcatttaga
      301 attagctgtt aattcattat tgcaggctga attaacagct tttcttgatt atgaaaaata
      361 tgatcgtgct gggttcaatt caggtaattc ccgtaatggg aattattcac gttcattcag
      421 aacagagtat ggagaattaa atttggtaat tcctagagat agaaatggta agttttctca
      481 acaaacttta cctgcttata aaagaaccaa tgactcttta gaaaccacta ttattcagct
      541 atttcaaaaa ggaattacta tgtctgaaat ctctgaattg attgaaaaaa tgtacgggca
      601 tcactatacg ccacaaacca tttcaaatat gactaaaata gtttctgaag atattattgc
      661 ctttaaagaa aggtccttag aatccagata ctctgtcatt tttatggatg ctactcacat
      721 tcctttaaaa agacaaactg tttcaaaaga agcagtttat attgtaattg gcattcgatt
      781 agatggaacc aaagaggttc ttgggtttag tcttgctcca actgaatctg cttatgtttg
      841 gaaagagatt cttcaagacc taaaagaccg tggtttaaaa gaggttttat tagtcgtaac
      901 tgatggttta agtggcatta acgatagtat tcatagtgtt tatccaaatg ctcaatttca
      961 acaatgttgt gtccatatat ctagaaatat cgctcataag gttcgtgtta gtgatcgaca
     1021 agaaatctgt agcgatttca
//
LOCUS       DACDKM010000260         1012 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20834_2016
            SAMN12640370-rid9891663.denovo.270, whole genome shotgun sequence.
ACCESSION   DACDKM010000260 DACDKM010000000
VERSION     DACDKM010000260.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640370
            Sequence Read Archive: SRR10040891
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1012)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1012)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742835.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742835.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:04:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,012
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 29 of 75
            Pseudo Genes (incomplete)         :: 33 of 75
            Pseudo Genes (internal stop)      :: 19 of 75
            Pseudo Genes (multiple problems)  :: 5 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1012
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640370-rid9891663.denovo.270"
                     /isolate="E20834_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            36..881
                     /locus_tag="IV334_002901"
     CDS             36..881
                     /locus_tag="IV334_002901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAP8944896.1"
                     /translation="MDEMKLIGIDLDGTLLNSDQSISQKNARTMKHLPEDCFPFICSG
                     REVEDINNILKKSGLRIPAVGLNGSVGFDGEKKIFESSFDKNDVKKIDSVLSKFPTKI
                     YTNLGSYESQNYKNKMKKIFHEVGSEFSIKELNYELEYEKSICSIPYNNIEEIIEKDA
                     ISIYKFFIFIPNRQLKANIFNHLKKMLNVSVTESAPVNIEIVPQNVSKGVVFDRLESI
                     YNLKKPLRIAIGDSLNDLSMFESADISFAMGNSHQEIMKKADYVTVSNDKDGVSQAFE
                     RILTI"
BASE COUNT          394 a          126 c          177 g          315 t
ORIGIN      
        1 ataagcagtg aatttataat caacgtatag gagacatgga tgaaatgaaa ctaattggta
       61 tagatttaga cggtactctt ttgaattctg accagagcat tagtcaaaaa aatgcaagaa
      121 caatgaaaca tttaccggaa gactgttttc cattcatttg ttctggaaga gaagtagaag
      181 acattaataa tattctcaaa aaatctggtt taaggatacc agcagtaggg ttaaatggtt
      241 ctgttggatt tgatggagaa aaaaagatat ttgagtcttc cttcgataaa aatgatgtaa
      301 aaaaaattga ttctgtccta tcgaaatttc caaccaaaat atatacaaat ctcggaagtt
      361 atgaatctca gaattataaa aataaaatga aaaaaatttt tcacgaggta gggagtgaat
      421 tttctatcaa agagctaaac tatgagcttg agtatgaaaa atctatttgt tcaatccctt
      481 ataataatat tgaggaaatt atagaaaagg atgctatcag catttacaaa ttttttattt
      541 ttattcctaa tcgtcaattg aaagctaata tatttaacca tttgaaaaag atgctaaatg
      601 tgtctgttac tgaatcggca cctgtaaata ttgaaatcgt acctcaaaat gtatctaaag
      661 gtgtggtgtt tgatcgtttg gaatcaatct acaatttaaa gaaacctttg agaatcgcga
      721 tcggagatag tttaaatgac ttatctatgt ttgaaagtgc agatattagt tttgcaatgg
      781 gaaatagtca tcaagaaatc atgaaaaaag ctgattatgt cactgtttca aatgataaag
      841 acggagtatc tcaagcattt gaaagaattc ttaccatata aactaattaa ggtagtatag
      901 aacatagaat tctaaatacg gaaaaatgga gcataaattc cagtacaaat agtacttctt
      961 cagtgtcaaa cgaagcgaat ataacataga tatttaggat tgtgacaaaa at
//
LOCUS       DACDKM010000261          994 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20834_2016
            SAMN12640370-rid9891663.denovo.271, whole genome shotgun sequence.
ACCESSION   DACDKM010000261 DACDKM010000000
VERSION     DACDKM010000261.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640370
            Sequence Read Archive: SRR10040891
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 994)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 994)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742835.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742835.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:04:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,012
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 29 of 75
            Pseudo Genes (incomplete)         :: 33 of 75
            Pseudo Genes (internal stop)      :: 19 of 75
            Pseudo Genes (multiple problems)  :: 5 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..994
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640370-rid9891663.denovo.271"
                     /isolate="E20834_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(<1..>994)
                     /locus_tag="IV334_002902"
     rRNA            complement(<1..>994)
                     /locus_tag="IV334_002902"
                     /product="16S ribosomal RNA"
BASE COUNT          209 a          307 c          225 g          253 t
ORIGIN      
        1 agcacgtgtg tagcccaggt cataaggggc atgatgattt gacgtcatcc ccaccttcct
       61 ccggtttgtc accggcagtc ttgctagagt gcccaactga atgatggcaa ctaacaataa
      121 gggttgcgct cgttgcggga cttaacccaa catctcacga cacgagctga cgacaaccat
      181 gcaccacctg tcactttgcc cccgaagggg aagctctatc tctagagtgg tcaaaggatg
      241 tcaagacctg gtaaggttct tcgcgttgct tcgaattaaa ccacatgctc caccgcttgt
      301 gcgggccccc gtcaattcct ttgagtttca accttgcggt cgtactcccc aggcggagtg
      361 cttaatgcgt tagctgcagc actgaagggc ggaaaccctc caacacttag cactcatcgt
      421 ttacggcgtg gactaccagg gtatctaatc ctgtttgctc cccacgcttt cgagcctcag
      481 cgtcagttac agaccagaga gccgccttcg ccactggtgt tcctccatat atctacgcat
      541 ttcaccgcta cacatggaat tccactctcc tcttctgcac tcaagtctcc cagtttccaa
      601 tgaccctccc cggttgagcc gggggctttc acatcagact taagaaaccg cctgcgctcg
      661 ctttacgccc aataaatccg gacaacgctt gccacctacg tattaccgcg gctgctggca
      721 cgtagttagc cgtggctttc tggttagata ccgtcaaggg atgaacagtt actctcatcc
      781 ttgttcttct ctaacaacag agttttacga tccgaaaacc ttcttcactc acgcggcgtt
      841 gctcggtcag actttcgtcc attgccgaag attccctact gctgcctccc gtaggagttt
      901 gggccgtgtc tcagtcccaa tgtggccgat caccctctca ggtcggctat gcatcgtggc
      961 cttggtgagc cgttacctca ccaactagct aatg
//
LOCUS       DACDKM010000262          993 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20834_2016
            SAMN12640370-rid9891663.denovo.272, whole genome shotgun sequence.
ACCESSION   DACDKM010000262 DACDKM010000000
VERSION     DACDKM010000262.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640370
            Sequence Read Archive: SRR10040891
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 993)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 993)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742835.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742835.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:04:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,012
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 29 of 75
            Pseudo Genes (incomplete)         :: 33 of 75
            Pseudo Genes (internal stop)      :: 19 of 75
            Pseudo Genes (multiple problems)  :: 5 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..993
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640370-rid9891663.denovo.272"
                     /isolate="E20834_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(24..911)
                     /locus_tag="IV334_002903"
     CDS             complement(24..911)
                     /locus_tag="IV334_002903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HAP8944897.1"
                     /translation="MQELKTRSARHHRPKRKMVINKKAIAPLIGTSLLVGPMMVTAVP
                     QHVHADEQGSQTVADPQAFIDQIGWSASEVAASNDLYASVMIAQALLESAYGTSGLAS
                     APNYNLFGVKGSYNGQVVYMATKEYVNGQWVTMNEPFRKYSSYWESFQDHANVLRSTS
                     FSTGDYHYAGVWKSNTTSFYDATAYLTGRYATDPNYGAKLNSLINTYNLTRFDTPSTG
                     SATTTTSTTTTTTTTTSTSSSSTTDVNTNNGQTYTVVSGDTLWDIAQKFGMSVDDLMA
                     KNGMSMSSYSLLVGQELNV"
BASE COUNT          287 a          207 c          179 g          320 t
ORIGIN      
        1 atttctttgc ctttttatct aaatcaaacg tttagttctt gaccaactaa taaagaataa
       61 ctggacatag acatgccatt tttcgccatt agatcatcaa cagacatgcc aaatttttgt
      121 gcaatatccc atagcgtatc acctgacacg actgtatatg tctgaccatt atttgtattt
      181 acatctgttg ttgaagatga tgacgttgaa gtcgtcgtag tagtcgttgt tgttgtactt
      241 gtggttgttg tagccgaacc tgttgatggc gtatcaaaac gggttaaatt ataagtatta
      301 atcaatgagt tcaatttggc cccataattt ggatcggtag cataacgacc tgtcaagtac
      361 gctgttgcat cataaaaact tgttgtattg cttttccaca cgccagcata atgataatcg
      421 cccgtggaaa aactggttga acgaagaaca ttcgcatggt cttggaagga ttcccaataa
      481 gaagaatatt ttcggaatgg ctcattcatc gttacccatt gtccgtttac atattctttg
      541 gttgccatat aaacgacctg tccgttatag cttcctttca caccgaatag attataattt
      601 ggcgcactag ctaatccgga agtgccatat gcactttcca acaacgcttg agcgatcatg
      661 actgaagcat acaagtcatt acttgcagca acttctgacg cagaccagcc gatttgatcg
      721 ataaacgctt gaggatcagc gactgtttga gatccttgtt catctgcatg aacatgttgc
      781 ggtaccgctg taaccatcat cggcccaact aaaagactcg ttccaattaa aggagcaatc
      841 gcttttttat tgatcaccat ttttctttta ggtctatggt gccgggcact gcgggtcttc
      901 agctcttgca ttcaaattcc tcctcaaaat aaaaaaactt aataaaaagt tctcttctta
      961 aaacaaactt aattaattaa attatacttt taa
//
LOCUS       DACDKM010000263          988 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20834_2016
            SAMN12640370-rid9891663.denovo.273, whole genome shotgun sequence.
ACCESSION   DACDKM010000263 DACDKM010000000
VERSION     DACDKM010000263.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640370
            Sequence Read Archive: SRR10040891
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 988)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 988)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742835.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742835.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:04:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,012
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 29 of 75
            Pseudo Genes (incomplete)         :: 33 of 75
            Pseudo Genes (internal stop)      :: 19 of 75
            Pseudo Genes (multiple problems)  :: 5 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..988
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640370-rid9891663.denovo.273"
                     /isolate="E20834_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..704
                     /locus_tag="IV334_002904"
     CDS             <1..704
                     /locus_tag="IV334_002904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HAP8944898.1"
                     /translation="YSVIFMDATHIPVKRQTVSKEAVYITIGIRLDGTKEVLGFTIAP
                     TESAYIWKEVLQDLRKRGLEEVLLVVTDGLSCIEESIHSVYPNAQFQQCCVHVSRNIA
                     HKVRVRGRKEICEDFKLVYQANSKEEALDHIDFMTRKWKKQYPRVVNLLLNPALLTFY
                     NFPHAIRRTIYSTNLIEGFNKQLKRYTRRKEQFPNEESLERFFISQFNQYNQKFLGRI
                     HKGFKEIQDTLESMI"
BASE COUNT          335 a          161 c          163 g          329 t
ORIGIN      
        1 attattccgt tatatttatg gatgcgacgc atatccctgt gaagcgacag actgtttcga
       61 aggaagcagt gtatattacg attggtattc gtttggatgg aaccaaagaa gttcttgggt
      121 ttactattgc cccaactgag tctgcttata tttggaaaga agttcttcaa gatcttagaa
      181 aacgtgggtt agaagaagtt ttattagtag tgacagacgg attaagctgt attgaagaaa
      241 gtatccatag tgtgtatccg aatgcccaat ttcaacaatg ttgtgtgcat gtatctagaa
      301 atatcgctca taaagttcgt gttcgaggtc gaaaagaaat ttgtgaggat ttcaaattgg
      361 tttaccaagc gaattcaaaa gaagaggcat tggatcacat cgactttatg actaggaaat
      421 ggaaaaagca gtatccaaga gtcgtcaatt tactcttgaa tcctgcccta ttaacctttt
      481 ataatttccc tcacgccatc agacgaacaa tttattcgac gaacctgatt gaaggcttta
      541 ataagcagct aaaacgatat actcgaagaa aagaacaatt ccctaatgaa gaatctctag
      601 agagattctt catttctcaa tttaatcaat ataaccaaaa atttttaggt agaattcaca
      661 agggatttaa agaaattcag gatacattag agtcgatgat ttaactgtaa ttaacagaat
      721 ggattttcca tttacatata attcttgacg ctaccataat ctaaataacc atcatattca
      781 tctactactt atataattga gttttattaa actttttaag tttctattct atcaaatatt
      841 taaacaatct ctgcatccga aaagactacc tcgtatagtt ttactaacaa tatttttatt
      901 agcgtgttca aatcctgatt tcttgtatca attattttct gttcactttc aataagaaaa
      961 aagtctttac ttccatggat aataataa
//
LOCUS       DACDKM010000264          985 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20834_2016
            SAMN12640370-rid9891663.denovo.274, whole genome shotgun sequence.
ACCESSION   DACDKM010000264 DACDKM010000000
VERSION     DACDKM010000264.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640370
            Sequence Read Archive: SRR10040891
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 985)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 985)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742835.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742835.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:04:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,012
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 29 of 75
            Pseudo Genes (incomplete)         :: 33 of 75
            Pseudo Genes (internal stop)      :: 19 of 75
            Pseudo Genes (multiple problems)  :: 5 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..985
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640370-rid9891663.denovo.274"
                     /isolate="E20834_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            6..623
                     /locus_tag="IV334_002905"
     CDS             6..623
                     /locus_tag="IV334_002905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8944899.1"
                     /translation="MGFQVLFGSLILTIVFLKWKTSNKKSMWSLVGIVLIIFTVTAAT
                     EANYSHKIQSYKGRLVMTKKDVTRYTSSRTGGLYDYAPLDALKYKDHLKKHEVKVGDR
                     WENASHKAYNSKIVYTVEDADEQNITLPVFNYWGTVVKVNGKKSTIENDNGLVSIKGT
                     GKRTIVEVSSTYPTTMILSLLISSITFIFLFTRYLIRCCGEMKID"
BASE COUNT          359 a          127 c          164 g          335 t
ORIGIN      
        1 gaaccttggg ttttcaagtg ctttttggtt cattaatctt aaccatagtg tttttaaagt
       61 ggaaaacaag caacaaaaaa agtatgtgga gccttgtagg aatagttctt attattttta
      121 ctgtgactgc agcaacagaa gcaaattatt cgcataaaat tcagtcgtat aaaggtcgtt
      181 tagttatgac caaaaaagat gttacacgtt atacatcctc aagaactgga ggcctatatg
      241 actatgcacc attagatgca ttgaaatata aagatcattt aaagaaacat gaagtaaaag
      301 ttggtgatcg gtgggagaat gcatctcata aagcatataa ttccaaaata gtctatacag
      361 tagaagatgc cgatgagcaa aatatcacat tacctgtatt caattattgg ggaacagttg
      421 tgaaagtaaa tgggaaaaag agcactatag aaaatgacaa tggtttagtg tctattaaag
      481 gtacggggaa aagaactata gttgaggttt catctacgta tccaactacg atgattctat
      541 ctttattaat tagttcaata acttttatat tcttatttac acgttattta ataagatgct
      601 gcggtgaaat gaaaatagat tagatagata ttataaatat gttgattcta ttttcatagc
      661 aatggagaaa atacattagc aatatataaa agaaggtatc aagagtcaca gtgaatttat
      721 tccgaaaagt gaaaagagca aatataaaat ttaatgaaaa tatagaacta aataggaaaa
      781 cgtagacgta attttctacg ttttcctttt catatatatt aaatattacg gaaaattata
      841 taaactaatt tagtgttttg tttgtaattc ctaattttgc tccactttta gataattttt
      901 aggtctattt aactcatcag acattctatg ctcatttttc tccaaatttg tttcaactct
      961 ttcgtactta attttgcgta tttga
//
LOCUS       DACDKM010000265          943 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20834_2016
            SAMN12640370-rid9891663.denovo.275, whole genome shotgun sequence.
ACCESSION   DACDKM010000265 DACDKM010000000
VERSION     DACDKM010000265.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640370
            Sequence Read Archive: SRR10040891
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 943)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 943)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742835.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742835.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:04:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,012
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 29 of 75
            Pseudo Genes (incomplete)         :: 33 of 75
            Pseudo Genes (internal stop)      :: 19 of 75
            Pseudo Genes (multiple problems)  :: 5 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..943
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640370-rid9891663.denovo.275"
                     /isolate="E20834_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            2..>943
                     /locus_tag="IV334_002906"
     rRNA            2..>943
                     /locus_tag="IV334_002906"
                     /product="23S ribosomal RNA"
BASE COUNT          293 a          187 c          274 g          189 t
ORIGIN      
        1 attgtgtgaa tacatagcac attcgaggta gacgcagaga actgaaacat ctaagtacct
       61 gcaggaagag aaagaaaatt cgattccctg agtagcggcg agcgaaacgg gaaaagccca
      121 aaccagcaag cttgcttgtt ggggttgtag gactccaata tggtagttct ttcagatagt
      181 cgaatgactt ggaaaagtca gtcaaagagg gtaaaaaccc cgtagacgaa atgtggaaga
      241 cacctaggag gatcctgagt acggcggaac acgagaaatt ccgtcggaat ccgggaggac
      301 catctcccaa ggctaaatac tccctagtga ccgatagtga accagtaccg tgagggaaag
      361 gtgaaaagca ccccggaagg ggagtgaaat agaacctgaa accgtgtgcc tacaacaagt
      421 caaagcccgt taatgggtga tggcgtgcct tttgtagaat gaaccggcga gttacgattg
      481 catgcgaggt taagttgaag agacggagcc gcagcgaaag cgagtctgaa tagggcgttt
      541 gagtatgtag tcgtagaccc gaaaccatgt gatctaccca tgtccaggtt gaaggtgcgg
      601 taaaacgcac tggaggaccg aacccacgta cgttgaaaag tgcggggatg aggtgtgggt
      661 agcggagaaa ttccaaacga acttggagat agctggttct ctccgaaata gctttagggc
      721 tagcctcgga attgagaatg atggaggtag agcactgttt ggactagggg cccatctcgg
      781 gttaccgaat tcagataaac tccgaatgcc attcattcat atccgggagt cagactgtga
      841 gtgataagat ccatagtcga aagggaaaca gcccagacca ccagctaagg tcccaaaata
      901 tatgttaagt ggaaaaggat gtggggttgc acagacaact agg
//
LOCUS       DACDKM010000266          942 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20834_2016
            SAMN12640370-rid9891663.denovo.276, whole genome shotgun sequence.
ACCESSION   DACDKM010000266 DACDKM010000000
VERSION     DACDKM010000266.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640370
            Sequence Read Archive: SRR10040891
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 942)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 942)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742835.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742835.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:04:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,012
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 29 of 75
            Pseudo Genes (incomplete)         :: 33 of 75
            Pseudo Genes (internal stop)      :: 19 of 75
            Pseudo Genes (multiple problems)  :: 5 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..942
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640370-rid9891663.denovo.276"
                     /isolate="E20834_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..365
                     /locus_tag="IV334_002907"
     CDS             <1..365
                     /locus_tag="IV334_002907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HAP8944900.1"
                     /translation="VLQTQVKSMRNAMQYLEYMDFFVKEFDTNLYVAGVIPFMLESGD
                     AVDQEMYKQAQEIYGEHLIKNVVLKNARLKRYDGSGITMEKTKKGLLKQWDRKSHELF
                     ISILKELEEHENWYAEVE"
     gene            366..914
                     /locus_tag="IV334_002908"
     CDS             366..914
                     /locus_tag="IV334_002908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8944901.1"
                     /translation="MADIKGLDIFGNNKGLAKIKKEAEAKKKQQAITPSENKQLEVHQ
                     VQEIKTSSNVERQSRGRTKKVGAPIRKFDKVYSTKHPLKLSTLLNATSRVLVEKYETN
                     MTRDELLRKALDEYIKQNLTQEDKQDLFNDVVKELDLFREKYPIVPELDDRGEIIRTT
                     SQIKQDTEDSLRKGWGMNNKVR"
BASE COUNT          378 a          133 c          200 g          231 t
ORIGIN      
        1 tcgtcttaca gacccaagta aaatcgatga gaaatgcgat gcaatatctt gagtatatgg
       61 acttttttgt gaaagaattt gatacaaatt tatatgttgc tggtgttatt cctttcatgt
      121 tggaaagcgg tgacgcagta gatcaggaaa tgtataaaca agcccaagag atttatgggg
      181 aacatttgat taaaaatgtt gtactcaaaa atgcgcgctt aaaacgatac gacggatcag
      241 gtattacgat ggagaagaca aagaaaggtc tcttaaaaca atgggatcgc aaaagccacg
      301 aattatttat cagtattttg aaagaattgg aagaacacga gaattggtat gcggaggtgg
      361 agtaattggc tgacatcaaa ggacttgata tttttgggaa caacaaagga ctcgccaaaa
      421 taaaaaaaga agcagaagca aagaaaaagc agcaagcaat tacaccatct gaaaataagc
      481 aattagaagt gcaccaagtt caagaaataa aaacttcctc caatgttgaa agacagagta
      541 gaggaagaac aaaaaaagtt ggcgcaccaa tcagaaagtt tgataaggtt tattccacca
      601 agcatccttt aaaactgtcg actttgttaa atgcgacttc cagagtttta gtagaaaagt
      661 atgagactaa tatgacaaga gatgaattgc ttagaaaagc tttagatgag tatattaaac
      721 agaatcttac gcaagaagat aagcaagatt tgtttaatga cgtggtgaaa gaactagact
      781 tgttcagaga aaaatatcca attgttccag aattagatga tcgtggagaa ataataagaa
      841 ctaccagtca gataaaacaa gataccgaag actcactgcg caaagggtgg ggaatgaata
      901 ataaagttag ataataatca aagaagaact aagtaactac ct
//
LOCUS       DACDKM010000267          927 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20834_2016
            SAMN12640370-rid9891663.denovo.277, whole genome shotgun sequence.
ACCESSION   DACDKM010000267 DACDKM010000000
VERSION     DACDKM010000267.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640370
            Sequence Read Archive: SRR10040891
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 927)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 927)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742835.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742835.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:04:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,012
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 29 of 75
            Pseudo Genes (incomplete)         :: 33 of 75
            Pseudo Genes (internal stop)      :: 19 of 75
            Pseudo Genes (multiple problems)  :: 5 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..927
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640370-rid9891663.denovo.277"
                     /isolate="E20834_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..788
                     /gene="tnpB"
                     /locus_tag="IV334_002909"
     CDS             <1..788
                     /gene="tnpB"
                     /locus_tag="IV334_002909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS200/IS605 family element transposase accessory
                     protein TnpB"
                     /protein_id="HAP8944902.1"
                     /translation="ALIDSKFIKVPKLKSLVRIKLHRQPKGIIKSATISRHSSGKYYI
                     SLLCKEEIIELPKTNSTIGIDLGIMDFAILSDGQKIDNNKFTSKMEKKLKREQRKLSR
                     RALLAKNKGTNLFEAKNYQKQKRKVARLHEKVMNQRTDFLNKLSTEMIKNHDIICIED
                     LNTKGMLRNHKLAKSISDVSWSKFVTKLQYKADWYGRKIIKVDKWFPSSQICSECGHK
                     DGKKSLEIREWTCPVCHTHHDRDINASINILTEGLRLHSMGLA"
BASE COUNT          341 a          146 c          168 g          272 t
ORIGIN      
        1 ttgctttgat tgatagtaaa ttcatcaaag ttcctaaatt gaagtcatta gtaagaatta
       61 agcttcatag acaaccaaaa gggattatca aatccgctac aatatcacgt cattctagcg
      121 gtaaatacta tatttctttg ttgtgtaaag aagaaattat cgaattacct aaaactaatt
      181 ccacaattgg tattgactta ggtattatgg actttgcgat tctttctgat ggacaaaaaa
      241 ttgataataa taaattcacg tccaaaatgg aaaagaaact aaagcgtgaa caacgcaaat
      301 tgtctaggcg tgctttattg gctaaaaata aaggtaccaa tctatttgaa gctaaaaatt
      361 atcaaaaaca aaaacgcaaa gtagctagat tgcatgaaaa agtaatgaat caacgtactg
      421 attttctgaa taagttgagt acagaaatga tcaaaaatca cgatattatc tgtattgaag
      481 acttaaacac aaaaggtatg ttgcgaaatc ataaattagc taaaagtatt tctgatgttt
      541 cttggtcaaa gtttgtgact aaattacaat acaaggctga ctggtatgga cgaaaaatca
      601 tcaaagtaga taaatggttt ccgtctagtc agatttgttc agagtgcgga cataaagatg
      661 gcaagaaatc tcttgaaatt cgagaatgga cttgtcctgt ttgtcatact catcatgatc
      721 gtgatatcaa tgctagtatc aatattctga ccgaaggtct aagactacat tctatgggat
      781 tggcttagat ccaatcatca agaaccgtag gaactacggg gatagcttgg taaataagag
      841 acaccgctgt tagtaaagaa atacgctatc aagtatgctc tattcccaag aagctcccac
      901 ttcaagcgtt aagaaccgtc aggttta
//
LOCUS       DACDKM010000268          924 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20834_2016
            SAMN12640370-rid9891663.denovo.278, whole genome shotgun sequence.
ACCESSION   DACDKM010000268 DACDKM010000000
VERSION     DACDKM010000268.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640370
            Sequence Read Archive: SRR10040891
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 924)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 924)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742835.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742835.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:04:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,012
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 29 of 75
            Pseudo Genes (incomplete)         :: 33 of 75
            Pseudo Genes (internal stop)      :: 19 of 75
            Pseudo Genes (multiple problems)  :: 5 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..924
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640370-rid9891663.denovo.278"
                     /isolate="E20834_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            138..752
                     /locus_tag="IV334_002910"
     CDS             138..752
                     /locus_tag="IV334_002910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001261742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAP8944903.1"
                     /translation="MRVAYIRVSSIDQNEQRQIEEMKKFGAERIFIEKQSGATITHRP
                     VFQEALDFVRDQDIFIVEAIDRLGRNYDEIIASVNYLKKKNVQLIITSLPIMAEAIGN
                     PLLDKFIKDLIIQILAMIAEQERTESKRRQAQGIKIAKANGVYKGRPKLYSANAKDPQ
                     RRLVYKNIVEDLKKGVAIAKIAKDYNVTRQTVYRIKKDSMVNHE"
     gene            complement(776..>924)
                     /locus_tag="IV334_002911"
     CDS             complement(776..>924)
                     /locus_tag="IV334_002911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016610020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HAP8944904.1"
                     /translation="KTTGKVLLDQLTTIDYEARECIFLEKAHDSLVEELLMKVRTVFQ
                     KVSK"
BASE COUNT          309 a          172 c          166 g          277 t
ORIGIN      
        1 atactatttc ctccatcatt agtttttgtc tatataaggt ataccctaac tagacacgac
       61 cgcgacattg aaaaatctgg ttttctcgtg tatgattagg gtgtttttta aatagttgca
      121 gaaagtaggt aaaaattttg agagttgcat acatacgtgt ctcatccatc gatcaaaatg
      181 aacaacggca gattgaagaa atgaaaaaat tcggtgcaga gagaattttc attgagaaac
      241 aatctggcgc aacgattact catcgacctg tttttcaaga agcactggat ttcgtccgag
      301 atcaagatat ttttattgtg gaagctattg atcgacttgg aagaaattac gacgagatca
      361 tcgcttcagt caattacttg aagaagaaaa atgtgcagct gatcatcaca agtctaccga
      421 tcatggccga agcaattggc aatcctctcc tggataaatt cattaaagat ttgatcattc
      481 aaattttggc catgatcgct gaacaagaac gtaccgaatc taaacgacgt caggcacaag
      541 gcattaagat tgcaaaagcg aacggtgttt ataaaggacg tcctaagcta tacagtgcca
      601 atgcaaaaga tccccaacgt cgtcttgttt ataaaaacat cgttgaggat ctaaaaaaag
      661 gtgttgcgat cgctaagatt gcaaaagact acaatgttac gagacaaacc gtatatagaa
      721 taaaaaaaga cagcatggtc aatcacgaat agttgatcat gctgctgttt ctattttatt
      781 tgcttacttt ttgaaaaact gttctaactt tcatcaataa ctcctcaact aaactatcat
      841 gcgctttttc caaaaaaata cactctctgg cttcataatc aattgtcgtt agttgatcta
      901 ataatacttt tcctgttgtc ttca
//
LOCUS       DACDKM010000269          890 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20834_2016
            SAMN12640370-rid9891663.denovo.279, whole genome shotgun sequence.
ACCESSION   DACDKM010000269 DACDKM010000000
VERSION     DACDKM010000269.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640370
            Sequence Read Archive: SRR10040891
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 890)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 890)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742835.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742835.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:04:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,012
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 29 of 75
            Pseudo Genes (incomplete)         :: 33 of 75
            Pseudo Genes (internal stop)      :: 19 of 75
            Pseudo Genes (multiple problems)  :: 5 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..890
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640370-rid9891663.denovo.279"
                     /isolate="E20834_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..888
                     /locus_tag="IV334_002912"
     CDS             <1..888
                     /locus_tag="IV334_002912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element ISEnfa3 family transposase"
                     /protein_id="HAP8944905.1"
                     /translation="RNLDSRTRFEQEEIKVKTILQLKKEFKLNLLLSIAQLAKSTYYY
                     WVKKLDKPDKYSKIKQEITAIVKESRNSYGYRRVTLALKMKGYTINHKTVRKLMSQMG
                     LTCQIRIKRYKSYKGTVGKIAKNVLKRNFSVDTPNKKWVTDVTEFKIKGRKIYLSPIL
                     DLFNGEIISYSISTSPTYKLIEEMLQQAIKKKGTEGSLILHSDQGWQYQMPQYQKKLK
                     ENNIIQSMSRKGNCLDNSVIENFFGVLKSEFFYREKFRSIEIFQSKLNEYIRWYNNKR
                     IKLKLNGLSPVEYRKQSIK"
BASE COUNT          386 a          116 c          144 g          244 t
ORIGIN      
        1 agaaaccttg attcaagaac aagatttgag caagaagaaa tcaaggttaa aacaatcctt
       61 cagctcaaaa aagaatttaa attgaatcta ttactttcaa ttgcacaatt agcgaagtca
      121 acttattatt actgggtaaa aaaattagat aagccagata aatatagcaa gataaagcaa
      181 gaaattacag cgattgtaaa agaatcaaga aactcttatg gttatcgtag agtcacttta
      241 gcgttaaaga tgaaaggata tacaatcaat cataaaacag ttagaaaatt aatgtcccaa
      301 atgggactta cctgtcaaat ccgaataaag cgatataaat cttataaagg aacagtagga
      361 aaaattgcca agaatgtgtt aaaacgaaat ttttcagtag atacacccaa taaaaaatgg
      421 gtgacagacg tcactgaatt caagataaaa ggaagaaaaa tctatctatc tcctatactt
      481 gatttattta acggagaaat aattagttat agtatatcaa ctagtccaac atataaactg
      541 attgaagaaa tgcttcaaca agcaattaaa aaaaagggaa ctgagggttc actgattcta
      601 cattcagatc aaggatggca gtatcagatg ccacaatatc aaaaaaaatt aaaagagaat
      661 aacattattc aaagcatgtc tagaaaagga aattgcttag ataattctgt aatagaaaat
      721 ttttttggtg tgcttaaatc agagtttttt tatcgagaaa aattccgatc aattgagata
      781 tttcaaagta aattaaatga gtatattagg tggtataata acaaaaggat aaaactaaag
      841 ttaaatggac tatctccagt agaatatcgg aaacagtcca ttaaatagtt
//
LOCUS       DACDKM010000270          865 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20834_2016
            SAMN12640370-rid9891663.denovo.281, whole genome shotgun sequence.
ACCESSION   DACDKM010000270 DACDKM010000000
VERSION     DACDKM010000270.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640370
            Sequence Read Archive: SRR10040891
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 865)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 865)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742835.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742835.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:04:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,012
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 29 of 75
            Pseudo Genes (incomplete)         :: 33 of 75
            Pseudo Genes (internal stop)      :: 19 of 75
            Pseudo Genes (multiple problems)  :: 5 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..865
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640370-rid9891663.denovo.281"
                     /isolate="E20834_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(<1..76)
                     /locus_tag="IV334_002913"
     CDS             complement(<1..76)
                     /locus_tag="IV334_002913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HAP8944906.1"
                     /translation="MSKINPLTLITLNIFFPVIIFLGKG"
     gene            complement(206..790)
                     /locus_tag="IV334_002914"
     CDS             complement(206..790)
                     /locus_tag="IV334_002914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAP8944907.1"
                     /translation="MESGLSHFEKEGFQQTKIRQLCLTAGVTTGAFYKHFTSKEALFE
                     EIIQPHINALINMYNEEFTKYLLNLENIDIEKSWRENFNELDPFINYIFENKRIFDLI
                     LFQSAGTKYQDIVEKITSFVTKKTVCCLDKLKTIGMINQNLVFNEEEIHFYIYSFYAT
                     FYDILKHSYSKEKTMLLTRRLYQFSVPGWIELLS"
BASE COUNT          289 a          145 c          118 g          313 t
ORIGIN      
        1 ttccctttcc taaaaaaata atgaccggaa aaaaaatatt taaagtaatc aaggttaacg
       61 gattgatttt tgacactctt tcacctccaa aaaataagat cgcactgtta tcttaagctg
      121 ataaaaatgc accgttattc agataacagt gcgatcttat caaaaagcat aactgagaac
      181 aattctcaat gcaagctttt tttaattaac tcagtaattc aatccaccca ggaacagaaa
      241 attgatacaa tcttctggtt aatagcatag tcttttcttt tgaatagcta tgcttcaata
      301 tatcatagaa tgtagcgtag aatgagtaaa tgtagaagtg gatttcttct tcattaaaga
      361 ctaaattttg attgatcatt ccgatagtct ttaacttatc tagacaacag actgtctttt
      421 tcgtcacaaa tgatgtaatt ttttcaacaa tatcttgata tttggttcca gctgattgga
      481 ataaaatcaa atcgaatata cgtttattct caaaaatata attaataaat ggatctaatt
      541 cattgaagtt ctctctccaa cttttctcta tatctatatt ttctaagttt aagaggtatt
      601 tggtaaactc ttcattatac atattaataa gcgcattaat atgcggctga ataatttctt
      661 caaataacgc ttccttagaa gtaaaatgtt tataaaaagc acccgtagtt acgcctgcgg
      721 tcaggcacag ttgccttatc ttagtttgct gaaatccttc tttttcaaaa tggcttagtc
      781 cactttctat taacttttgg tgagttataa tataatctct ggtttctatg ggtacttcct
      841 cctaataaga tgaattaact ttatt
//
LOCUS       DACDKM010000271          858 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20834_2016
            SAMN12640370-rid9891663.denovo.282, whole genome shotgun sequence.
ACCESSION   DACDKM010000271 DACDKM010000000
VERSION     DACDKM010000271.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640370
            Sequence Read Archive: SRR10040891
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 858)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 858)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742835.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742835.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:04:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,012
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 29 of 75
            Pseudo Genes (incomplete)         :: 33 of 75
            Pseudo Genes (internal stop)      :: 19 of 75
            Pseudo Genes (multiple problems)  :: 5 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..858
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640370-rid9891663.denovo.282"
                     /isolate="E20834_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..>858
                     /locus_tag="IV334_002915"
     CDS             <1..>858
                     /locus_tag="IV334_002915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HAP8944908.1"
                     /translation="FSIKNFPNAKEIFKALDDSTIKKVGIIGAGYIGVEIAEAIRKRG
                     KEVYLFDVADRVLSTYYDRSFSDKVEEILSKNGIHLCLSESPKKYLGRKKIEKIVTTS
                     GTQYSMDMVVQAIGFLSNSPIGEKELLRDVSGAYLTNEYQQTNIKDIYAIGDCATTFF
                     TSINRSSVDFSISNALRTAYIATQHINNQDFTPSGSQFTNGFSIFNYNFYAAGLNLKT
                     ARLLSESIDYIEIEDFIRPAFLRKNSRVKLRISFEKNTKRIVGVQLCSQEDLSGLLSM
                     FSLAIEEQIT"
BASE COUNT          324 a          106 c          150 g          278 t
ORIGIN      
        1 ttttcgataa agaattttcc aaatgcaaaa gagattttta aagctttgga tgatagtact
       61 attaaaaaag taggaattat tggagcagga tatattggag ttgaaattgc tgaagccata
      121 agaaaaagag ggaaagaagt atatcttttc gatgttgcag atagagtgtt gtcgacttat
      181 tacgaccgta gtttttcaga taaagttgaa gaaatactat caaaaaatgg aatacatctt
      241 tgtctatcag aaagtcctaa aaaatattta ggaagaaaaa agattgaaaa aatagtcact
      301 acaagtggta cccaatactc tatggatatg gtcgtacaag ctataggatt tctatctaat
      361 agtccaattg gggaaaagga actcttgaga gatgttagtg gagcatatct aactaatgaa
      421 taccagcaaa caaatataaa agatatatat gctattgggg attgtgctac aacttttttt
      481 acgtccatta atagaagtag tgttgatttt tctatttcta atgctctaag aacagcttat
      541 attgcaactc aacatattaa caatcaagat tttacacctt ctggttccca atttacaaat
      601 ggcttttcta tatttaatta caatttttac gcagcaggtt tgaatttaaa aacagcacga
      661 ttattaagtg agtctataga ttatatagaa atagaagatt tcattagacc tgcttttttg
      721 agaaaaaata gtcgtgtaaa attaagaatt agctttgaaa aaaatacaaa gagaatagta
      781 ggtgtacaac tttgtagtca agaagattta tctggactat tatcaatgtt ttctcttgct
      841 atagaagaac aaattaca
//
LOCUS       DACDKM010000272          856 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20834_2016
            SAMN12640370-rid9891663.denovo.283, whole genome shotgun sequence.
ACCESSION   DACDKM010000272 DACDKM010000000
VERSION     DACDKM010000272.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640370
            Sequence Read Archive: SRR10040891
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 856)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 856)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742835.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742835.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:04:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,012
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 29 of 75
            Pseudo Genes (incomplete)         :: 33 of 75
            Pseudo Genes (internal stop)      :: 19 of 75
            Pseudo Genes (multiple problems)  :: 5 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..856
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640370-rid9891663.denovo.283"
                     /isolate="E20834_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            83..>856
                     /locus_tag="IV334_002916"
     CDS             83..>856
                     /locus_tag="IV334_002916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAP8944909.1"
                     /translation="MQGVIAMMSKNNTNGRNQFAMLTIDDLVPQDHLVRKIDAALDFE
                     FIYPIVEATYSDLGRPSIDPVILIKLVFIQYLFGIRSMRQTIKEVDTNVAYRWFLGYS
                     FEEKIPHFSTFGKNYVRRFRETTVFEDIFAYILEQAVKAGFVTEDNFHLDSTHIKANA
                     NKHNFTKEMTHDEAKAYQDELEDEINRQRIEAGKRPFTLDLEKEVKLKERKISKADPE
                     SGYYVKGEQEKQFAYSAHTSCDDNGFILSTIITPGNIHDS"
BASE COUNT          285 a          158 c          165 g          248 t
ORIGIN      
        1 ataaaatgcc agactgtaga caaagtccat tttatggatg atgtctacag tcttttcttt
       61 tataatagat ttatgaaaac gaatgcaagg agtgatagct atgatgtcta aaaacaatac
      121 gaatggtaga aatcaatttg cgatgcttac gattgatgat ctagttcctc aagatcatct
      181 cgttcgaaag attgatgccg cgcttgattt tgaatttatt tatccaatcg tcgaagccac
      241 ttactccgat ttaggtcgcc caagtatcga cccagtgatt ctgatcaaat tggtgtttat
      301 tcagtattta ttcggtatcc gttccatgcg acaaaccatt aaggaagtcg ataccaatgt
      361 cgcttaccgc tggtttttag gctactcctt cgaagagaaa atcccccatt tctcaacttt
      421 tggaaagaat tatgttcgcc gatttagaga aacgacggtg tttgaggata tttttgccta
      481 tattttagaa caagcagtta aagcaggttt tgtaacagag gataattttc atctagattc
      541 tacccacatc aaagctaacg ccaataaaca taattttacg aaagaaatga cccatgatga
      601 agcgaaagcc tatcaggatg aattagaaga tgagatcaat cgtcaacgaa tagaggctgg
      661 aaaacgccct tttactttgg acttagaaaa ggaagtgaag ctgaaagaaa gaaaaataag
      721 taaagctgat ccggaaagcg gctactacgt caaaggcgaa caagaaaaac aatttgctta
      781 ttctgcccat acttcatgcg acgacaatgg atttattctt agcacaatca tcactcctgg
      841 aaatattcat gacagt
//
LOCUS       DACDKM010000273          813 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20834_2016
            SAMN12640370-rid9891663.denovo.284, whole genome shotgun sequence.
ACCESSION   DACDKM010000273 DACDKM010000000
VERSION     DACDKM010000273.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640370
            Sequence Read Archive: SRR10040891
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 813)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 813)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742835.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742835.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:04:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,012
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 29 of 75
            Pseudo Genes (incomplete)         :: 33 of 75
            Pseudo Genes (internal stop)      :: 19 of 75
            Pseudo Genes (multiple problems)  :: 5 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..813
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640370-rid9891663.denovo.284"
                     /isolate="E20834_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..>813
                     /locus_tag="IV334_002917"
     CDS             <1..>813
                     /locus_tag="IV334_002917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP8944910.1"
                     /translation="YDGLAKLKDAKVVSGEQRINREDLSDEFKNVVSLEATNNTKRNI
                     LFSSGVFTIKEGKNIEENDKNSIIVHEEFAKQNNLKLGDEVDLELLDIEKSGKIKSHK
                     FKIIGIFSGKKQETYTGLSSDFSENMVFVDYSTSQEILNNSENNEIANKILMYSGSAE
                     STDLALNKLKELKIDESKYFVEKDSNAFEESLESVSGIKYIIKVMTYSIMLGGMVVLS
                     LILILWLRERIYEIGIFLSIGTSKIQIIMQFIFELIFISIPSIISSLFLGNV"
BASE COUNT          334 a           70 c          138 g          271 t
ORIGIN      
        1 atatgatgga ttagcaaaac taaaagatgc taaagtagtt agcggagaac aaagaataaa
       61 tagagaagat ttatctgacg aatttaagaa tgttgtttca ctcgaagcta caaataatac
      121 taaaagaaat attttattta gtagtggagt atttacaatt aaagaaggta aaaatataga
      181 agaaaatgat aagaattcaa ttattgttca tgaagaattt gctaaacaaa acaatctaaa
      241 attaggtgat gaagttgatc ttgaattact agatattgaa aaaagtggaa aaataaaaag
      301 tcataaattt aaaattatag ggatcttttc tggtaaaaaa caggaaacat atacaggatt
      361 atcttctgat tttagtgaaa atatggtgtt tgtagattat tcaacaagcc aagagatatt
      421 aaataattca gaaaataatg aaattgcaaa taaaatttta atgtattctg gtagtgcaga
      481 atctacagac ttagccttaa acaaattgaa agagcttaaa attgatgagt caaagtattt
      541 tgttgaaaaa gattctaatg cgtttgaaga gtctttagag tcagtgagtg gaataaaata
      601 tataataaaa gtaatgactt attctattat gttaggtggg atggttgttc tttcattaat
      661 cttgattcta tggttaaggg aaagaattta tgaaataggt atattcttat ctattggaac
      721 atctaagata caaattataa tgcaatttat attcgagtta atattcatat cgataccaag
      781 tataatatct tccttatttt taggaaatgt att
//
LOCUS       DACDKM010000274          810 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20834_2016
            SAMN12640370-rid9891663.denovo.285, whole genome shotgun sequence.
ACCESSION   DACDKM010000274 DACDKM010000000
VERSION     DACDKM010000274.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640370
            Sequence Read Archive: SRR10040891
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 810)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 810)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742835.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742835.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:04:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,012
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 29 of 75
            Pseudo Genes (incomplete)         :: 33 of 75
            Pseudo Genes (internal stop)      :: 19 of 75
            Pseudo Genes (multiple problems)  :: 5 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..810
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640370-rid9891663.denovo.285"
                     /isolate="E20834_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(<1..88)
                     /locus_tag="IV334_002918"
     CDS             complement(<1..88)
                     /locus_tag="IV334_002918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose uptake protein"
                     /protein_id="HAP8944911.1"
                     /translation="MIAIGQFVFYIPFFIMISILFYYIKWTKK"
     gene            246..509
                     /locus_tag="IV334_002919"
     CDS             246..509
                     /locus_tag="IV334_002919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8944912.1"
                     /translation="MDHVVLEIDLHLINNIRVIYYVVSNSVEQRVLTNKINGILAKKD
                     VHRFNNGEGSYYSIPVEKIIYTTVKVREDLDTKKAYEPIFTTY"
     gene            533..721
                     /locus_tag="IV334_002920"
     CDS             533..721
                     /locus_tag="IV334_002920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8944913.1"
                     /translation="MNPYEQLIQDLIDGKIEKIDVKREELMAFREAWLKLENRKYVVG
                     KASLNGNVTYHYDPTRLF"
BASE COUNT          305 a          106 c          163 g          236 t
ORIGIN      
        1 tcttttttgt ccattttatg taatagaaaa gaatgcttat cataataaaa aaaggaatat
       61 aaaaaacaaa ttgaccgatc gcgatcatac tattgcctcc gataaataaa gtccatgata
      121 caaaataaca ctttgtgcga aatggcgcaa gcagttttgt ggatttatcc ggtaaaatct
      181 gtatcgattt tgaaattaga ggagaaactt gctataatca aagtgaaaga aggagtggga
      241 ttttcatgga tcatgtagtg ttagaaatcg atttgcattt gatcaataat attcgggtga
      301 tttactatgt tgtaagtaac agtgtcgaac aacgtgtact gacgaataaa ataaatggta
      361 ttttagcaaa gaaagacgtt caccgattca ataatggtga aggaagttat tatagtatcc
      421 cagtagaaaa aattatttat acgacagtca aagtcagaga agatctagat accaaaaaag
      481 catatgaacc tatttttaca acttattgat tgacagcaga tggaggaaaa aaatgaatcc
      541 atatgaacaa cttattcaag atttgatcga tggaaaaatc gaaaagattg atgtaaaaag
      601 agaagaatta atggcttttc gagaagcgtg gctgaagttg gagaatcgta aatatgtggt
      661 gggaaaagcc tcactgaacg gcaatgtgac ttatcattac gatccgacac gtttatttta
      721 gtatgaagta ttcagaaaag aaccaaagga ggacgatgca gcagcattgt cctcctttga
      781 ttttacaaaa gtgaaagttt tacttatttg
//
LOCUS       DACDKM010000275          807 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20834_2016
            SAMN12640370-rid9891663.denovo.286, whole genome shotgun sequence.
ACCESSION   DACDKM010000275 DACDKM010000000
VERSION     DACDKM010000275.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640370
            Sequence Read Archive: SRR10040891
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 807)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 807)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742835.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742835.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:04:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,012
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 29 of 75
            Pseudo Genes (incomplete)         :: 33 of 75
            Pseudo Genes (internal stop)      :: 19 of 75
            Pseudo Genes (multiple problems)  :: 5 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..807
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640370-rid9891663.denovo.286"
                     /isolate="E20834_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(113..343)
                     /locus_tag="IV334_002921"
     CDS             complement(113..343)
                     /locus_tag="IV334_002921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="resolvase"
                     /protein_id="HAP8944914.1"
                     /translation="MDEAMDHPLLNKFIKDLILQIFSMIDEQEPTESKRHRAQGIIKI
                     AKRMFSIKGVLSYTVLIQKIPNHLVYKNNVED"
     gene            complement(573..>807)
                     /locus_tag="IV334_002922"
     CDS             complement(573..>807)
                     /locus_tag="IV334_002922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HAP8944915.1"
                     /translation="AKIIAITNYIHSPIGKSADLVLQTAIEEFLNGGSLAGKISQLYI
                     CDLLVHGYEQNNNIDSVELREKVLRSIIDKSIE"
BASE COUNT          276 a          131 c          127 g          273 t
ORIGIN      
        1 caaaaggatc ataaactttt aacaagtgat gaaatgggtg gaaattgatt cttatagaca
       61 gtttgtctca taacattgaa gtattttgca attttagcat tgaaacactt ttttaatctt
      121 caacgttgtt cttataaaca agatgattgg gaatcttttg aattaacact gtatagctta
      181 gaacaccctt tatagaaaac attcgcttag caatcttaat aattccttgt gcccgatgtc
      241 gtttagattc agtaggttcc tgctcatcga tcatggaaaa aatttgaagg atcagatcct
      301 taataaattt atttaagaga ggatgatcca ttgcttcatc catactcgat aaactagtga
      361 taatgagcta cacgtttttc tttcaagttg atcaatagct tccacgataa aaatatcttt
      421 agataaaatc aagaaccgta ggaactacag ggatatttgg tcaataagag ccaccgctat
      481 tagtaaaaaa taagctatca agtatgctct catcccaaga attgcctact tcaatcgtta
      541 aaaaccgtta ggtttagcta agtgggggta gttcactcaa tacttttatc aataatagag
      601 cgcaaaactt tttctcttaa ttcaactgaa tcaatattat tgttttgttc ataaccatga
      661 actaatagat cacaaatata aagttgtgat atttttccag ctaaagatcc accattaaga
      721 aattcttcaa tggctgtttg taaaactaag tctgcagact ttcctatagg tgaatgaata
      781 tagttagtta tagcaataat ttttgcg
//
LOCUS       DACDKM010000276          793 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20834_2016
            SAMN12640370-rid9891663.denovo.288, whole genome shotgun sequence.
ACCESSION   DACDKM010000276 DACDKM010000000
VERSION     DACDKM010000276.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640370
            Sequence Read Archive: SRR10040891
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 793)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 793)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742835.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742835.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:04:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,012
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 29 of 75
            Pseudo Genes (incomplete)         :: 33 of 75
            Pseudo Genes (internal stop)      :: 19 of 75
            Pseudo Genes (multiple problems)  :: 5 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..793
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640370-rid9891663.denovo.288"
                     /isolate="E20834_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            85..354
                     /locus_tag="IV334_002923"
     CDS             85..354
                     /locus_tag="IV334_002923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HAP8944916.1"
                     /translation="MEAVAYSNFRQNLRSYMKQVNEDAETLIVTSKDVEDTVVVLSKR
                     DYDSMQETLRTLSNNYVMEKIRRGDEQFSKGAFKTHDLIEVESDD"
     gene            347..604
                     /locus_tag="IV334_002924"
     CDS             347..604
                     /locus_tag="IV334_002924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HAP8944917.1"
                     /translation="MIKAWSDDAWDDYLYWHEQGNKSNIKKINKLIKDIDRSPFAGLG
                     KPEPLKHDLSGKWSRRITDEHRLIYRVENETIFIYSAKDHY"
BASE COUNT          304 a           91 c          151 g          247 t
ORIGIN      
        1 taagaagtta gaatattaat catttgctta attgtacaat ataatgtaca attgttttat
       61 agaaataaat aaggggtgaa aggaatggaa gcagtagctt attcaaattt ccgccaaaat
      121 ttacgtagtt atatgaaaca agttaatgag gatgctgaaa cacttattgt aacaagtaaa
      181 gatgtagaag atacagttgt tgtattatca aaaagagatt atgattctat gcaagaaacg
      241 ttgagaacac tttctaataa ttacgtcatg gaaaaaattc gtcgaggaga tgaacaattc
      301 tccaaaggtg catttaaaac acatgactta atcgaggttg aatctgatga ttaaggcttg
      361 gtctgatgat gcttgggatg attatcttta ttggcatgag caaggaaaca aaagcaatat
      421 aaaaaagatt aacaagttaa taaaagatat cgatcgttcc ccctttgctg gattaggaaa
      481 acctgagcca ttaaagcatg atttatctgg aaaatggtcc agaagaatta cagatgaaca
      541 tagactgata tatagagttg aaaatgaaac gatatttatt tattctgcaa aagatcacta
      601 ttaaccaatc ggaagtaagg aaagggtcag aaacttaaaa gtttttgatc cttattttat
      661 ttaccctagt catttaaaaa gctaatatag cttagtgttg attgttatta atgaatgtgt
      721 ttgttacgcg tattacggat ataaggttag taaaatcatt tctaaagttg aggaaaagta
      781 aatataaatg gct
//
LOCUS       DACDKM010000277          781 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20834_2016
            SAMN12640370-rid9891663.denovo.289, whole genome shotgun sequence.
ACCESSION   DACDKM010000277 DACDKM010000000
VERSION     DACDKM010000277.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640370
            Sequence Read Archive: SRR10040891
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 781)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 781)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742835.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742835.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:04:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,012
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 29 of 75
            Pseudo Genes (incomplete)         :: 33 of 75
            Pseudo Genes (internal stop)      :: 19 of 75
            Pseudo Genes (multiple problems)  :: 5 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..781
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640370-rid9891663.denovo.289"
                     /isolate="E20834_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            122..754
                     /locus_tag="IV334_002925"
     CDS             122..754
                     /locus_tag="IV334_002925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HAP8944918.1"
                     /translation="MIFPKVNEASLNKGSFFHIYENEDFELYNILSQKEVIENQIICH
                     PIIYKSIFKYAFFASNDFQRMEITDCIFENCDFSGCILTKSMLHRVKFVNCKLTGTKL
                     MESYIGNTLFENCKMDYVNLSGSNIKESTFEHSVLSSADFVDCSLTKIMFSTNDLENA
                     SFFDTDLKNIDLSSNSFEMIEVRSEHLKNCTLNQLQALGFARKFLQIKVV"
BASE COUNT          279 a          108 c          125 g          269 t
ORIGIN      
        1 cgtttcaaga gatagcgaca ctatcgttat cgaaaaaggc gaaaacctat ctcattttct
       61 gctaatgtaa gttggtttag cttttgttaa tatgacttta tgaaaatgga ggtaattaaa
      121 tatgattttt cctaaagtta atgaagcaag cttgaataaa ggatcctttt ttcatatcta
      181 tgaaaacgaa gattttgaac tgtataatat cctttcacaa aaagaggtca tcgaaaacca
      241 aataatctgc catccaatta tttacaaatc tattttcaag tatgcatttt ttgcttccaa
      301 tgattttcaa agaatggaga ttaccgactg tatattcgaa aactgtgatt tttcaggctg
      361 tatattgaca aaaagcatgt tgcatcgcgt gaaatttgtc aattgtaaat taacaggtac
      421 taaattgatg gaaagttata ttgggaacac gttattcgag aattgtaaaa tggactatgt
      481 aaatttatct ggttcaaaca taaaagaaag tacctttgag cactctgtct tatcaagcgc
      541 agattttgta gactgttccc tcactaaaat aatgttttct actaatgatt tagaaaatgc
      601 tagctttttt gatactgatt taaaaaatat cgatttaagt agcaactcct ttgaaatgat
      661 tgaagtacgc tcggaacatc taaaaaattg tacgcttaat cagttgcaag cacttggttt
      721 tgctagaaaa tttttacaaa taaaagtagt ataatcaaaa tgaatatata agataaattt
      781 t
//
LOCUS       DACDKM010000278          771 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20834_2016
            SAMN12640370-rid9891663.denovo.290, whole genome shotgun sequence.
ACCESSION   DACDKM010000278 DACDKM010000000
VERSION     DACDKM010000278.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640370
            Sequence Read Archive: SRR10040891
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 771)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 771)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742835.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742835.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:04:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,012
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 29 of 75
            Pseudo Genes (incomplete)         :: 33 of 75
            Pseudo Genes (internal stop)      :: 19 of 75
            Pseudo Genes (multiple problems)  :: 5 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..771
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640370-rid9891663.denovo.290"
                     /isolate="E20834_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(118..>771)
                     /locus_tag="IV334_002926"
     CDS             complement(118..>771)
                     /locus_tag="IV334_002926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element ISEfa8 family transposase"
                     /protein_id="HAP8944919.1"
                     /translation="GRIMKRRGLTSTYTIAHFKGQRTACNEAKTANVLDRTFTQEQPL
                     EAIVTDLTYVRVGKKWHYICLILDLFNREIIGYSCGEKKDASLVKEAFGRIPYSLTDV
                     KLFHTDRGKEFDNQTIHEILNGFGITRSLSRKGCPYDNAVVESTYKSVKVEFVHQYQF
                     ETLAQLRLELFDYVHWWNYLRLHGTLAYETPIQIRQQRLAKRILDNERGSDTSGEAA"
BASE COUNT          213 a          176 c          145 g          237 t
ORIGIN      
        1 actttttttg acaaaaatta taaggtgttg tgaatgctgc ggtgttatgg ctacgattgc
       61 ccttgacaat gagcatcctc ggatctgatt ttctccggca gaagcacaat cattcaatta
      121 cgctgcctct ccagaggtat cagatccgcg ctcattatca aggattcgct tcgccaatct
      181 ctgttgtcga atttggatcg gtgtttcata cgccaacgtg ccatgtaagc gtagatagtt
      241 ccaccaatgc acataatcaa acaattctag acgtagctgt gccagtgtct caaattggta
      301 ttgatgcacg aattctactt tgacagattt ataggttgat tccacaacgg cattatcata
      361 cggacaaccc ttcctactca atgaacgagt aattccaaaa ccattcagaa tctcatgaat
      421 ggtttggtta tcaaattcct ttccccggtc tgtatgaaaa agcttgacgt ctgttaaaga
      481 atacggtatc cgtccaaagg cttcttttac caatgaggca tctttcttct caccacagga
      541 ataaccaata atttctcgat taaacaaatc aagtattaag cagatataat gccacttttt
      601 ccccacgcga acataagtaa gatccgtaac gatggcttcc aatggctgtt cttgtgtaaa
      661 ggtccgatct aatacattcg ctgttttcgc ttcattacaa gctgttcgtt gccctttaaa
      721 atgagcgatc gtataggtag atgtcaatcc gcggcgtttc atgattcgac c
//
LOCUS       DACDKM010000279          755 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20834_2016
            SAMN12640370-rid9891663.denovo.291, whole genome shotgun sequence.
ACCESSION   DACDKM010000279 DACDKM010000000
VERSION     DACDKM010000279.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640370
            Sequence Read Archive: SRR10040891
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 755)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 755)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742835.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742835.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:04:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,012
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 29 of 75
            Pseudo Genes (incomplete)         :: 33 of 75
            Pseudo Genes (internal stop)      :: 19 of 75
            Pseudo Genes (multiple problems)  :: 5 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..755
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640370-rid9891663.denovo.291"
                     /isolate="E20834_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(<1..626)
                     /locus_tag="IV334_002927"
     CDS             complement(<1..626)
                     /locus_tag="IV334_002927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAP8944920.1"
                     /translation="MDNHTRKLLGLTDKHLFFDEEWLIEKEYKGVQAQFIKGRLDDSP
                     SHCEHCGSIRIIRNGSYTTRTQILKVKEKLTILELKRTRFLCYDCGQTFSAKTDLVDE
                     HHQLTKELKQAILIELYENQSRKLIAKKYFVSDGTVTRILREATKHYQPRMNFLPTVL
                     CMDEFKSMKSVSGSMSFICVDGTTHQLFTILEDRRLYKLTQYFLRFPR"
BASE COUNT          224 a          148 c          145 g          238 t
ORIGIN      
        1 tttctaggaa agcgcaggaa gtattgagtt agtttgtaaa gtctgcgatc ttcaaggatc
       61 gtaaatagct gatgggttgt cccatcaaca caaataaaac tcatggaacc agaaacagac
      121 ttcattgatt tgaactcatc catacacaac accgtcggaa ggaagttcat acgtggctgg
      181 taatgcttcg ttgcttcacg caagatccgt gtcaccgtcc catccgagac gaagtacttc
      241 ttagcgatta gtttacgtga ttgattttcg tacagttcta tgagaatcgc ctgcttcagt
      301 tcctttgtta gttgatgatg ttcatcaact aagtccgttt tcgcagaaaa ggtctgccca
      361 caatcatagc atagaaaacg ggtacgtttt aattctaaaa tagtcaactt ttctttgact
      421 ttcaatatct gagtacgtgt cgtataggaa ccgttgcgga taatacgaat cgatccacag
      481 tgttcacaat gggaaggtga gtcatccaat ctccctttaa taaactgcgc ttgaacgcct
      541 ttatattctt tttcaatcag ccattcttca tcaaaaaata aatgtttatc tgttaatccg
      601 agtaattttc tagtatgatt atccatagag cgactccttt ggtagggtga tgttttggtc
      661 gactaaattt taccaaaagt gagtctcttt ttgagttgaa acgaagaaaa agtgttagta
      721 gaaaatcgcc ttcgattttc caacaacaca aaata
//
LOCUS       DACDKM010000280          745 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20834_2016
            SAMN12640370-rid9891663.denovo.292, whole genome shotgun sequence.
ACCESSION   DACDKM010000280 DACDKM010000000
VERSION     DACDKM010000280.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640370
            Sequence Read Archive: SRR10040891
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 745)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 745)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742835.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742835.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:04:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,012
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 29 of 75
            Pseudo Genes (incomplete)         :: 33 of 75
            Pseudo Genes (internal stop)      :: 19 of 75
            Pseudo Genes (multiple problems)  :: 5 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..745
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640370-rid9891663.denovo.292"
                     /isolate="E20834_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            33..656
                     /locus_tag="IV334_002928"
     CDS             33..656
                     /locus_tag="IV334_002928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8944921.1"
                     /translation="MFVIWEVVKSRVQEMNEHFSVSFAENPSKYFRRLVFLLILSLLL
                     TVAMFLLPEKIREEQVNSLVYSSIPTSKLKPFDYSKADQKIKQSRAISVMFSKPNGKT
                     YQDMLDVFNDPELMEELNRPIFYYPIVYDVHELEQKYNIRTDEVTFIFFDGGKEANRI
                     TAGKGGITDFDKELIPELNRLPLANIKQLEEELDRTGVTSQNSEIAN"
BASE COUNT          266 a          113 c          156 g          210 t
ORIGIN      
        1 aataaaaaat aggagaaaaa gtagaaaaga aggtgtttgt tatttgggaa gtagttaaat
       61 cgagagtaca agaaatgaat gaacatttct cagtgtcatt cgcagaaaat ccaagtaagt
      121 attttcgacg tcttgttttt ctgctcatct tatcattgct gttaacagtt gcaatgtttt
      181 tattgccgga aaaaatccga gaagagcaag taaatagtct cgtttattcg tccataccaa
      241 ccagcaaatt gaaacctttt gattacagca aagctgatca gaaaatcaaa caaagccggg
      301 cgatatcggt gatgttcagc aaaccgaacg ggaaaaccta tcaggacatg cttgatgttt
      361 ttaatgatcc tgagcttatg gaagaattga atcgaccgat cttttattat ccgatcgttt
      421 atgatgtaca tgaactggaa caaaaatata acatacgaac ggatgaagtg acatttattt
      481 tctttgatgg aggaaaagaa gcaaatcgga taactgcggg taagggagga ataactgatt
      541 ttgataagga gttgatccct gaactgaatc gacttccgtt agccaatatc aaacaattag
      601 aagaagaatt ggatagaacg ggagtaactt cccaaaattc cgagattgct aattgagaca
      661 ttggcgcgat gaaacggtgg gtctatatag cgctattttt aattatcaga acaagtgatg
      721 tttgatcttc aaaataacaa ataaa
//
LOCUS       DACDKM010000281          742 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20834_2016
            SAMN12640370-rid9891663.denovo.293, whole genome shotgun sequence.
ACCESSION   DACDKM010000281 DACDKM010000000
VERSION     DACDKM010000281.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640370
            Sequence Read Archive: SRR10040891
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 742)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 742)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742835.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742835.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:04:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,012
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 29 of 75
            Pseudo Genes (incomplete)         :: 33 of 75
            Pseudo Genes (internal stop)      :: 19 of 75
            Pseudo Genes (multiple problems)  :: 5 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..742
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640370-rid9891663.denovo.293"
                     /isolate="E20834_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(<1..206)
                     /locus_tag="IV334_002929"
     CDS             complement(<1..206)
                     /locus_tag="IV334_002929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016610020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HAP8944922.1"
                     /translation="MVRKPRQGDILLLNTAPRSGHEQTGKRPYIVLSHDIIADYSNVV
                     TVAPISSTTRNYPLYVNINSEYEM"
     gene            complement(200..463)
                     /locus_tag="IV334_002930"
     CDS             complement(200..463)
                     /locus_tag="IV334_002930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PbsX family transcriptional regulator"
                     /protein_id="HAP8944923.1"
                     /translation="MEMLPIKKWGNSNGLRLPKYIMEYLGIHTEDKVKIIQEEVNGQK
                     RLIIEAANIENELTIEQLFENYTDERNHVTIQNLGEAVGNEKW"
BASE COUNT          251 a          123 c           90 g          278 t
ORIGIN      
        1 ttcatttcat attctgagtt tatatttaca tatagcggat agtttctagt tgtactagaa
       61 attggagcaa ctgtaacaac gttactataa tccgcaataa tatcatgact taaaacaata
      121 taaggtcttt tacctgtctg ttcgtgtcca gatcgaggtg ctgtattcaa taatagaata
      181 tcgccttgtc taggttttct caccattttt cattacctac tgcttcacct aaattttgaa
      241 tcgtcacatg atttctttca tcagtgtaat tttcaaataa ctgttcaatt gttaattcat
      301 tttctatatt agccgcttcg ataattagcc gcttctgacc gtttacttct tcttgtatta
      361 tcttcacttt gtcttctgta tgaattccaa gatattccat aatatacttt ggcaatctaa
      421 ggccattact atttccccat ttttttatag gtaacatttc catagtagac acctctttct
      481 gtatatacat agtataacat acataagaaa aaacgtatat acttaattat ccttttctat
      541 taaattagat taacattcca tgaatccata aactatgtta acatttatat gaaaatagtc
      601 gaaaggaaat cataatatgt ttaagaaaac aaaatcttta gaatcaggta agaaaaaaat
      661 tgatctaaaa ataatctctt ctggatactt taaagatttc atagttattg atacaaaatg
      721 gaataaaaag gacatagaaa at
//
LOCUS       DACDKM010000282          738 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20834_2016
            SAMN12640370-rid9891663.denovo.294, whole genome shotgun sequence.
ACCESSION   DACDKM010000282 DACDKM010000000
VERSION     DACDKM010000282.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640370
            Sequence Read Archive: SRR10040891
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 738)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 738)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742835.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742835.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:04:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,012
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 29 of 75
            Pseudo Genes (incomplete)         :: 33 of 75
            Pseudo Genes (internal stop)      :: 19 of 75
            Pseudo Genes (multiple problems)  :: 5 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..738
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640370-rid9891663.denovo.294"
                     /isolate="E20834_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..>738
                     /gene="acm"
                     /locus_tag="IV334_002931"
     CDS             <1..>738
                     /gene="acm"
                     /locus_tag="IV334_002931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Acm"
                     /protein_id="HAP8944924.1"
                     /translation="ADAGRDISSNVTSLTVDPTNITDGGNIKVKFSFDEKKQNIQPGD
                     YLWINWPSEGNIRGEGFQKEIPLMIENKNVGTLTVRKDSAQVVFNENIKNLDSVEGWG
                     QFEIQARNVTDTGEENTGPFTVTSGDKTATVNVTKPASGSSSSVFYYKTGDMLPEDTK
                     HIRWFLNINNNGTYVEQPVKISDEIQSGQRLDPSTFEINQIHLGEQKVYRGEEGIQQF
                     LQDFPSATFNFSVTDNYIEITIPKNFVN"
BASE COUNT          278 a          120 c          149 g          191 t
ORIGIN      
        1 gcggatgcag gcagagatat cagcagtaat gtcacttcgt tgaccgtgga cccaacgaat
       61 attacagatg gaggaaacat aaaagtaaaa ttctcgtttg acgaaaaaaa acaaaatatc
      121 caaccaggtg attatctttg gatcaattgg ccaagtgaag gaaatattcg cggtgaagga
      181 ttccaaaaag aaatcccatt aatgattgaa aataaaaatg ttggaacttt aacggtcaga
      241 aaagattctg cgcaggttgt gtttaatgaa aatattaaaa acctagactc agtagaaggt
      301 tgggggcaat ttgagataca ggccagaaac gtaaccgata ccggtgaaga aaatacggga
      361 ccattcaccg tgacgagcgg tgataaaaca gctactgtaa atgtgacaaa gccagcttct
      421 ggttcttctt ctagcgtttt ttattataaa acaggagaca tgctacctga agatactaag
      481 cacattcgat ggtttttgaa tattaacaat aacggcactt atgttgaaca accagttaaa
      541 atcagtgatg aaatccaaag tggacaaaga ttagatccaa gtacgtttga aattaatcaa
      601 atacacttag gtgaacagaa ggtttataga ggagaagagg gaatccaaca atttttacaa
      661 gatttcccca gcgctacatt taatttttca gtaactgata attacattga aataactata
      721 ccaaaaaact ttgtcaat
//
LOCUS       DACDKM010000283          722 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20834_2016
            SAMN12640370-rid9891663.denovo.296, whole genome shotgun sequence.
ACCESSION   DACDKM010000283 DACDKM010000000
VERSION     DACDKM010000283.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640370
            Sequence Read Archive: SRR10040891
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 722)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 722)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742835.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742835.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:04:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,012
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 29 of 75
            Pseudo Genes (incomplete)         :: 33 of 75
            Pseudo Genes (internal stop)      :: 19 of 75
            Pseudo Genes (multiple problems)  :: 5 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..722
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640370-rid9891663.denovo.296"
                     /isolate="E20834_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..>722
                     /locus_tag="IV334_002932"
     CDS             <1..>722
                     /locus_tag="IV334_002932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YSIRK signal domain/LPXTG anchor domain surface
                     protein"
                     /protein_id="HAP8944925.1"
                     /translation="AGEGTSIESGTTVFAVKDGVSLPEDKLPVLKAKDGYTDAKWPEE
                     ATQPITADDTEFVSSATKLDDIIENPGENIPAGYHKVTFTAGEGTSIESGTTVFAVKD
                     GVSLPEDKLPVLKAKDGYTDAKWPEEATQPITADDTEFVSSATKLDDKSDADKYNPEG
                     QKVTTELNKEPDAFEGIKNKEDLPKDTKYTWKEKVDVSAAGNKKGTVVVTYPDGSSDE
                     VEVDVTVTDNRSDADKYEPTVEG"
BASE COUNT          292 a          116 c          167 g          147 t
ORIGIN      
        1 gcaggagaag gaacaagtat tgaaagtgga acaacagtct ttgcagtgaa agacggcgta
       61 agcttaccag aagataaact tccagtgttg aaagcaaaag atggttatac agatgcgaaa
      121 tggccagaag aagcaactca accaattaca gcagatgata cagaatttgt atcaagtgca
      181 acaaaactgg atgatatcat tgaaaatcca ggagaaaaca ttcctgcagg ctaccacaaa
      241 gtaaccttca ccgcaggaga aggaacaagt attgaaagtg gaacaacagt ctttgcagtg
      301 aaagacggcg taagcttacc agaagataaa cttccagtgt tgaaagcaaa agatggttat
      361 acagatgcga aatggccaga ggaagcaact caaccaatta cagcagatga tacagaattt
      421 gtatcaagtg caacaaaact ggatgataaa tctgacgctg acaaatataa tcctgaaggt
      481 caaaaagtga ctacagaatt gaataaggaa ccagacgcat ttgagggaat taagaacaaa
      541 gaagatttac caaaagatac taagtatact tggaaagaaa aagtagatgt tagcgcagct
      601 ggaaataaaa aagggactgt tgtagtgaca tatccagatg gatcatctga tgaagttgaa
      661 gtagatgtta cagtgacaga caatcgttct gacgccgata aatatgagcc aacagtagaa
      721 gg
//
LOCUS       DACDKM010000284          721 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20834_2016
            SAMN12640370-rid9891663.denovo.297, whole genome shotgun sequence.
ACCESSION   DACDKM010000284 DACDKM010000000
VERSION     DACDKM010000284.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640370
            Sequence Read Archive: SRR10040891
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 721)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 721)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742835.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742835.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:04:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,012
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 29 of 75
            Pseudo Genes (incomplete)         :: 33 of 75
            Pseudo Genes (internal stop)      :: 19 of 75
            Pseudo Genes (multiple problems)  :: 5 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..721
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640370-rid9891663.denovo.297"
                     /isolate="E20834_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            134..346
                     /locus_tag="IV334_002933"
     CDS             134..346
                     /locus_tag="IV334_002933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014834536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8944926.1"
                     /translation="MAVWQIQLKERGAYGLISLSDVLCRKEMPDLFSLDQIIAIIGAA
                     AVVITAVAKASDSILAWLKFKKEQKK"
     gene            508..>721
                     /locus_tag="IV334_002934"
     CDS             508..>721
                     /locus_tag="IV334_002934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000357244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8944927.1"
                     /translation="MDKFNRLTLINQFEILKALNPNEEKYYAEKIEILTEGYEYHYSE
                     IFENISDPLPEEDSRFVLDILNMYRDI"
BASE COUNT          260 a          104 c          125 g          232 t
ORIGIN      
        1 taaaaacact atatattccg aaaagtgagt atctccattc ataaaataat caacatattt
       61 aaaaaatcta attagctttt ttagtcaatc taaagttctt tattctatga tataatgtta
      121 ctgtagaaag tttgtggcgg tttggcaaat ccaattgaag gagcgtggtg cctatgggct
      181 tattagctta tcggacgtac tctgtagaaa ggaaatgcct gatttgttct cattggatca
      241 aatcattgct attatcggag cagcagcagt agttattact gccgtagcga aagctagcga
      301 ttcgatttta gcttggttaa agttcaaaaa agaacaaaaa aaatagagcc gtctcaactt
      361 ttggcgaggt agacgagctc taaagcttta tttacatgcc atccgctttg aacggactac
      421 actgggcatg tgacagcatg ccctttcatt tatattatat ctcattcttt tatataatga
      481 aagtaaataa atcatggagg ataaaatatg gataaattta atagattaac attaataaat
      541 cagtttgaaa ttttaaaagc tttaaatcct aacgaagaaa aatactatgc tgaaaaaata
      601 gaaatactaa cagaaggtta cgaatatcac tatagtgaaa tatttgaaaa tatttctgat
      661 ccattgccag aagaagattc gcggtttgtt ctagacatat taaatatgta tagagatatt
      721 a
//
LOCUS       DACDKM010000285          720 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20834_2016
            SAMN12640370-rid9891663.denovo.298, whole genome shotgun sequence.
ACCESSION   DACDKM010000285 DACDKM010000000
VERSION     DACDKM010000285.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640370
            Sequence Read Archive: SRR10040891
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 720)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 720)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742835.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742835.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:04:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,012
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 29 of 75
            Pseudo Genes (incomplete)         :: 33 of 75
            Pseudo Genes (internal stop)      :: 19 of 75
            Pseudo Genes (multiple problems)  :: 5 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..720
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640370-rid9891663.denovo.298"
                     /isolate="E20834_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            263..544
                     /locus_tag="IV334_002935"
                     /pseudo
     CDS             263..544
                     /locus_tag="IV334_002935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295604.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            618..>720
                     /locus_tag="IV334_002936"
     CDS             618..>720
                     /locus_tag="IV334_002936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
                     /protein_id="HAP8944928.1"
                     /translation="MNHFKGKQFKKDVIIVAVGYYLRYNLSYREVQEL"
BASE COUNT          259 a           91 c          125 g          245 t
ORIGIN      
        1 ttaaaagttt ttgatccttt attttcaagt ctgatttggc agcttttcgg cagctcattt
       61 atttatggtc agaaatagat gctgaaattt taatctatta gcaaatagtc cagaacgtat
      121 gaagaggagt cggatatcat ttgtcattaa caccataaat atttataaaa ttcgtgttca
      181 cctacaatta aatttaatat ataatattcg ttgtaagggc ttataatttt ttgttaatga
      241 acaagatagg aagaaacatt taatggacag ttttcagaga ttgtcactag taaatcaatt
      301 tgagttattg gctaaatttg aggaccaaaa cagatcttat tatgaaagaa aaatcgaaat
      361 attgagagaa ggttacgagt atcattatga tgatgaaatt tggagtgatc tttctgagcc
      421 atttccgaaa gaagattctc gatttgtatt agatgtattg aacatgtatc gagatatcaa
      481 ttttagtaaa tcaaagctaa acttagatga aaaccaaatt actaattcat actatactca
      541 tttcggttct gttgcaaagt tttccaaaaa atctatttta gtgtaaaatt gagaaaaaag
      601 acagagagga cagagtaatg aatcatttta aaggcaaaca attcaaaaaa gacgtcatta
      661 ttgtcgctgt tggttactac ctgcgttaca atctaagcta tcgtgaagtt caggaattgt
//
LOCUS       DACDKM010000286          718 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20834_2016
            SAMN12640370-rid9891663.denovo.299, whole genome shotgun sequence.
ACCESSION   DACDKM010000286 DACDKM010000000
VERSION     DACDKM010000286.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640370
            Sequence Read Archive: SRR10040891
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 718)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 718)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742835.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742835.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:04:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,012
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 29 of 75
            Pseudo Genes (incomplete)         :: 33 of 75
            Pseudo Genes (internal stop)      :: 19 of 75
            Pseudo Genes (multiple problems)  :: 5 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..718
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640370-rid9891663.denovo.299"
                     /isolate="E20834_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(<1..482)
                     /locus_tag="IV334_002937"
     CDS             complement(<1..482)
                     /locus_tag="IV334_002937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAP8944929.1"
                     /translation="MVAKKYVIGNFKGGVGKSTCAQMLGFESAVNEGKRTLIIDLDMQ
                     GNTSDVMNLTHMNFTNEEGGGEGEPIVYENTVTDVLINNLPPKEAVYKVINNLYILPA
                     DMSFEMYDDWIKDQYNDSLSQFKYMKEKLEPLFDEFDVIYLDVPPSISVYSKSAMYIA
                     "
BASE COUNT          228 a          133 c          104 g          253 t
ORIGIN      
        1 tccgcaatgt acatcgcgga tttactatat acagaaatag aaggtgggac atctaaatag
       61 atgacgtcaa actcatcaaa taatggttct agcttttctt tcatatattt gaattgggat
      121 aatgaatcat tatattggtc tttgatccaa tcatcataca tttcaaagga catgtctgct
      181 ggaagtatat agaggttatt gattacttta tagacagcct ctttaggtgg aagattgttt
      241 atcaacacgt cagtcacagt attttcgtaa acaatcggtt caccttctcc gcctccttct
      301 tcgttagtga agttcatatg cgttaagttc atcacatcag aagtatttcc ttgcatatcc
      361 aagtcaatta tcaaggttct ttttccttca ttaactgctg attcaaaccc taacatttgc
      421 gcgcacgtac ttttacctac gccaccttta aaattaccga ttacatattt cttcgctacc
      481 acgttcctac ctcttttctt ttagcaatat tcatttgata ctattataac atatttattt
      541 ttagttacta ccaaaaaaaa gatatatatg tactacctta taattaataa gtaaaaggta
      601 gtacataaat aaggaaaagc tgaaatatat tgatttatag ctttcctcaa agtaatgacg
      661 ttatttaatt tatcgagaag cagagaatac tcgcgacttt agtcatgtag acgaatca
//
LOCUS       DACDKM010000287          688 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20834_2016
            SAMN12640370-rid9891663.denovo.300, whole genome shotgun sequence.
ACCESSION   DACDKM010000287 DACDKM010000000
VERSION     DACDKM010000287.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640370
            Sequence Read Archive: SRR10040891
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 688)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 688)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742835.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742835.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:04:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,012
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 29 of 75
            Pseudo Genes (incomplete)         :: 33 of 75
            Pseudo Genes (internal stop)      :: 19 of 75
            Pseudo Genes (multiple problems)  :: 5 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..688
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640370-rid9891663.denovo.300"
                     /isolate="E20834_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(226..>688)
                     /locus_tag="IV334_002938"
     CDS             complement(226..>688)
                     /locus_tag="IV334_002938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="cell wall metabolism sensor histidine kinase
                     WalK"
                     /protein_id="HAP8944930.1"
                     /translation="TKGKEVTYQVYNNFKILYPDYVITLDDDLTKEVTLQIYRNHFEQ
                     LIIIILDNAVKYSTTRKEVHISISSTLNEFEIAIQDFGEGIPKEDLNKIFNRFYRVDK
                     ARARTKGGNGLGLSIAKQLVENYKGRILAESVVGQGTIFRIFIPIAKAEDK"
BASE COUNT          199 a          122 c           97 g          270 t
ORIGIN      
        1 gcaatagaaa attccctatt tttatttatt atttttaaac acgaatatta cagcatttta
       61 ttgttttttc gttcatctaa aagatactta catttaatta acggatgtta ttctatttaa
      121 ttgttcgcta gtttttttat aatgacatcc ctgaatttct cacttgcttg tattttgtac
      181 cttattcttt gttggatata taaaaaaaca agtaaagcac atgctttact tgtcttctgc
      241 ctttgcgata ggaataaaga ttctaaaaat tgtcccttgc cctactacac tttctgctaa
      301 gatacgtcct ttatagtttt caaccagttg tttagcgata gacaacccca gaccatttcc
      361 acccttcgtt ctagctcttg ctttatccac tcgataaaaa cgattgaaga tcttattaag
      421 gtcttctttg ggtattcctt cgccaaagtc ctgaatcgcg atctcaaatt cattcagagt
      481 ggaagaaata gagatatgga cttcttttcg tgtagttgaa tactttactg cgttatccaa
      541 tatgataatg attaattgtt caaagtgatt acgataaatt tgcagggtca cttcttttgt
      601 caaatcatca tctaaagtga tcacatagtc tggatataat atcttgaaat tattataaac
      661 ttgataggtg acttcttttc ctttagtt
//
LOCUS       DACDKM010000288          683 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20834_2016
            SAMN12640370-rid9891663.denovo.301, whole genome shotgun sequence.
ACCESSION   DACDKM010000288 DACDKM010000000
VERSION     DACDKM010000288.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640370
            Sequence Read Archive: SRR10040891
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 683)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 683)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742835.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742835.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:04:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,012
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 29 of 75
            Pseudo Genes (incomplete)         :: 33 of 75
            Pseudo Genes (internal stop)      :: 19 of 75
            Pseudo Genes (multiple problems)  :: 5 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..683
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640370-rid9891663.denovo.301"
                     /isolate="E20834_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(<1..>683)
                     /locus_tag="IV334_002939"
     CDS             complement(<1..>683)
                     /locus_tag="IV334_002939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAP8944931.1"
                     /translation="ADISLKSTIDKCKATVMYPPNGLPLIIKGNSGVGKSFLASLIYQ
                     YALDRKVIHNDAKFVVVNCADYANNPELLSAVLFGYKKGAFTGAEKDTAGMLSSANGG
                     YLFLDEVHNLSAENQEKLFLLMDSQKYRKLGESVQWEYANVRLILATTEDKNTSLLAT
                     FRRRIPAEITLPDYNNRSTSEKIQLLFEFLKDEAITIDSKIFCSIELFTDLLSKTFEG
                     NVGELRNEI"
BASE COUNT          215 a          127 c          101 g          240 t
ORIGIN      
        1 tgatttcatt cctcaattct ccaacatttc cttcaaatgt tttcgataaa agatcagtaa
       61 aaagttcaat cgaacaaaat atttttgaat caatcgtaat tgcttcatct ttaagaaatt
      121 caaataataa ttgaattttt tcagatgtac ttctattatt gtaatctggc agagtgattt
      181 cagctgggat gcgtcttcta aaagttgcta acaaagaggt atttttatct tctgttgtgg
      241 ctaagataag gcgaacattt gcatactccc attgcacact ttcaccaagt ttacgatact
      301 tttgagaatc cataagaaga aacaatttct cttggttttc tgcacttaga ttgtgaacct
      361 catctaaaaa aaggtatcct ccattagcag aagatagcat ccctgcagta tctttttcag
      421 ccccagtaaa ggctcctttt ttatatccaa ataaaactgc tgacaatagc tctggattat
      481 ttgcataatc tgcacaattc acaacaacaa attttgcgtc attatgaatt acttttctat
      541 caagagcata ctgatagatc aaacttgcta aaaaactttt tccaacgcca ctgtttcctt
      601 ttattattaa tgggaggcca ttagggggat acattacagt agctttgcat ttatctatag
      661 tggatttcaa agatatgtct gct
//
LOCUS       DACDKM010000289          671 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20834_2016
            SAMN12640370-rid9891663.denovo.302, whole genome shotgun sequence.
ACCESSION   DACDKM010000289 DACDKM010000000
VERSION     DACDKM010000289.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640370
            Sequence Read Archive: SRR10040891
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 671)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 671)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742835.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742835.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:04:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,012
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 29 of 75
            Pseudo Genes (incomplete)         :: 33 of 75
            Pseudo Genes (internal stop)      :: 19 of 75
            Pseudo Genes (multiple problems)  :: 5 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..671
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640370-rid9891663.denovo.302"
                     /isolate="E20834_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            1..570
                     /locus_tag="IV334_002940"
                     /pseudo
     CDS             1..570
                     /locus_tag="IV334_002940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347576.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
BASE COUNT          276 a           73 c          102 g          220 t
ORIGIN      
        1 ttggcacagg ttattttcca attatggatt ggaggaatat ttcttattga tgttatttta
       61 ttagctaaag gaacaataag tattactaat tttgtaatag tatttatgta cgctaacatg
      121 gtccctactt tatggactac tttaattagt attatttgga aatggcaaga ctattcagtg
      181 tcattaaata gatattacga atattattca aataacaaca aagtggctgg cacaaaaaaa
      241 ataaataaaa taaattctat tactggtcaa tcattaaatt tttcatataa caaaagaaag
      301 gtattaagca atataagttt ttcaactgaa atgaataaac tattcatgat atcaggagaa
      361 aatggaacag ggaaaacaac actgatgaaa ttatttacta aacagttgcc ggttagtaat
      421 aatcaattat tcgtcaacgg gatagatatt aatatgattg ataacgaatc attaagaagg
      481 ttaatttcaa tcgcaacaca aataccatat ataattaata gctccgtgaa ggagaatatt
      541 ttaatgggga ctaaaaacta tgataaaaaa aaattcagta aattaataga actattggga
      601 ataagtaaat ttatgacgaa attaggtatt actttggacg acaatattta tgatgaaaaa
      661 atattattat c
//
LOCUS       DACDKM010000290          665 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20834_2016
            SAMN12640370-rid9891663.denovo.303, whole genome shotgun sequence.
ACCESSION   DACDKM010000290 DACDKM010000000
VERSION     DACDKM010000290.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640370
            Sequence Read Archive: SRR10040891
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 665)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 665)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742835.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742835.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:04:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,012
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 29 of 75
            Pseudo Genes (incomplete)         :: 33 of 75
            Pseudo Genes (internal stop)      :: 19 of 75
            Pseudo Genes (multiple problems)  :: 5 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..665
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640370-rid9891663.denovo.303"
                     /isolate="E20834_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(65..>665)
                     /locus_tag="IV334_002941"
     CDS             complement(65..>665)
                     /locus_tag="IV334_002941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HAP8944932.1"
                     /translation="FGTNYAKDFLATIPVSIDRKVEVIESNESDKTPLPEKEVILQHS
                     SDNSEISAGRFTIWKAALKVIRPTLLFGYGETDFYGNSDAKFLKKVDLDTTDVNELKR
                     AHGNMHNGFLQVLVSSGIIAFICIYAFYVLNIFSLLKTFFRSKVNYAFLGIVLIFILS
                     IFIDEMVEAHVLFNKRDVISIVFWYYLGSISYLYLKKEN"
BASE COUNT          249 a          117 c           81 g          218 t
ORIGIN      
        1 ttgataaaca tttaatatat taatcaaatg taaaggagtc caagcaatga aagctgtctt
       61 catattaatt ctcctttttt aaatacaagt aagaaatgct tcccaaataa taccagaaaa
      121 caattgaaat gacatcgcgc ttattgaaaa gtacatgagc ctccaccatt tcatctataa
      181 aaatagatag tataaaaatt aaaacaatac caagaaacgc ataattcact ttacttctaa
      241 aaaatgtttt gagtaaacta aagatattta acacatagaa agcatagata catataaaag
      301 caattatacc acttgataca agtacttgaa gaaatccatt atgcatgttt ccatgtgctc
      361 tttttagttc attcacatct gtagtatcaa gatctacttt tttcaaaaat tttgcatcgc
      421 tattcccata gaaatcggtc tctccataac caaataacaa agttggtctt ataactttaa
      481 gcgcagcttt ccaaatcgta aaacgaccag ctgaaatttc ggagttatca ctagaatgct
      541 gtaatattac ttctttttct ggaagtggag ttttatctga ttcatttgac tctataactt
      601 ctacttttct atcaatcgat acaggaattg ttgctaaaaa atccttagca taattagtac
      661 caaaa
//
LOCUS       DACDKM010000291          650 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20834_2016
            SAMN12640370-rid9891663.denovo.304, whole genome shotgun sequence.
ACCESSION   DACDKM010000291 DACDKM010000000
VERSION     DACDKM010000291.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640370
            Sequence Read Archive: SRR10040891
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 650)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 650)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742835.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742835.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:04:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,012
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 29 of 75
            Pseudo Genes (incomplete)         :: 33 of 75
            Pseudo Genes (internal stop)      :: 19 of 75
            Pseudo Genes (multiple problems)  :: 5 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..650
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640370-rid9891663.denovo.304"
                     /isolate="E20834_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..579
                     /locus_tag="IV334_002942"
     CDS             <1..579
                     /locus_tag="IV334_002942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_465591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="linear amide C-N hydrolase"
                     /protein_id="HAP8944933.1"
                     /translation="LPLSPLHWLLADKEKSIVIESMKDGLHIYDNPVGVLTNNPSFDY
                     QLFNLNNYRVLSSETPKNNFSNQISLNAYSRGMGGIGLPGDLSSVSRFVKATFTKLNS
                     VSGDSESESISQFFHILGSVEQQKGLCDVGDGKYEYTIYSSCCNVDKGIYYYRTYEDS
                     QITAIDMNKEDLDSHKLISYPIIEKQQIKYIN"
BASE COUNT          233 a           85 c          106 g          226 t
ORIGIN      
        1 cttcctttat cccctttaca ttggctatta gctgataaag aaaaatcaat tgtcattgaa
       61 agtatgaaag atggacttca tatatatgat aaccctgtgg gcgttcttac caataatcct
      121 tcatttgact atcaattatt taatttaaac aattatcgtg tcttatcgag tgaaactcct
      181 aaaaataatt tttcaaatca aataagtttg aatgcctata gccgcggtat gggagggata
      241 ggcttgcctg gagatttatc ctcagtatct cgttttgtta aagcgacttt tacgaagctg
      301 aattctgtat ctggagattc agagtcagaa agtattagtc aatttttcca tatcttaggt
      361 tcagtagaac aacaaaaagg tttgtgtgat gtaggtgatg gaaaatatga atatacaatt
      421 tattcttctt gttgcaatgt tgacaaagga atctattatt atcgaacata tgaagacagt
      481 caaattactg caattgatat gaataaagaa gacttagata gtcataagtt aattagttat
      541 ccaattatag aaaaacaaca aattaaatat ataaattagt taatgtgttg tgattgatta
      601 tttaatatag atataataca aaaaggcaaa gatattttca aaacattttt
//
LOCUS       DACDKM010000292          649 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20834_2016
            SAMN12640370-rid9891663.denovo.305, whole genome shotgun sequence.
ACCESSION   DACDKM010000292 DACDKM010000000
VERSION     DACDKM010000292.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640370
            Sequence Read Archive: SRR10040891
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 649)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 649)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742835.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742835.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:04:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,012
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 29 of 75
            Pseudo Genes (incomplete)         :: 33 of 75
            Pseudo Genes (internal stop)      :: 19 of 75
            Pseudo Genes (multiple problems)  :: 5 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..649
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640370-rid9891663.denovo.305"
                     /isolate="E20834_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..>649
                     /locus_tag="IV334_002943"
     CDS             <1..>649
                     /locus_tag="IV334_002943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HAP8944934.1"
                     /translation="KSNCGRRPLVLPEEQSEYIQRKVVQGWTPDVIVGRAAFPISCSA
                     RTIYRMFKKGLFDSSDLPMKGKRKPNGHQERRGKQTFRRSIHEREKDYSQFSNEFGHL
                     EGDTIVGLKHKSAVITLVERLSKVIITLKPCGRQAIDIEKKLNQWFESVPKNLFKSIT
                     FDCGKEFSNWKQISNVNDIAIYFADPGTPSQRGLNENSNGLLRRDGLLKSMDFNSV"
BASE COUNT          213 a          111 c          134 g          191 t
ORIGIN      
        1 aaatcaaact gtggtagacg tccgcttgtt ttacccgagg aacaatcaga atatattcaa
       61 agaaaggttg ttcaaggatg gacacccgat gtgattgttg gtcgtgcagc gtttcctatt
      121 agttgttctg ctcgtaccat ttatcgtatg ttcaaaaagg ggctatttga ttcttctgac
      181 ttaccgatga aaggcaagcg taaaccgaat ggacatcaag aaagacgtgg aaaacaaact
      241 ttccgccgct ctattcatga acgtgaaaag gattatagcc aattctcaaa tgagtttggt
      301 caccttgaag gtgacactat cgtaggtctg aaacataaaa gtgctgtaat taccttagtt
      361 gaacgattat caaaagttat catcacattg aaaccgtgtg gtagacaagc gattgatatt
      421 gaaaaaaaat taaatcaatg gtttgaatct gtaccgaaaa acctattcaa atccatcact
      481 tttgattgtg gaaaggaatt ttcaaattgg aaacagatca gtaatgtcaa tgatattgcc
      541 atttatttcg ctgatccagg aacgccgtct caaagaggcc taaacgagaa ttctaacgga
      601 ttgttacgta gagatggttt attgaaatct atggatttca attcagtag
//
LOCUS       DACDKM010000293          643 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20834_2016
            SAMN12640370-rid9891663.denovo.306, whole genome shotgun sequence.
ACCESSION   DACDKM010000293 DACDKM010000000
VERSION     DACDKM010000293.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640370
            Sequence Read Archive: SRR10040891
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 643)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 643)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742835.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742835.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:04:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,012
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 29 of 75
            Pseudo Genes (incomplete)         :: 33 of 75
            Pseudo Genes (internal stop)      :: 19 of 75
            Pseudo Genes (multiple problems)  :: 5 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..643
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640370-rid9891663.denovo.306"
                     /isolate="E20834_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            79..282
                     /locus_tag="IV334_002944"
     CDS             79..282
                     /locus_tag="IV334_002944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin"
                     /protein_id="HAP8944935.1"
                     /translation="MKHCVILGILGTCLAGIGTGIDVDAATYYGNGLYCNKEKCWVNW
                     GQSWSEGLKRWGDNLFGSFSGGR"
     gene            282..632
                     /locus_tag="IV334_002945"
     CDS             282..632
                     /locus_tag="IV334_002945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HAP8944936.1"
                     /translation="MTSINIDHTKKVIHELYNSLMTRPNQTKALLDITDVLFQVYKKI
                     DTAKYPEILVNKLVNYIYIVGFDNKIRFIDNDEKLLIELGDISKKAGINSKYKANFTD
                     KSQFYSYSETMPQR"
BASE COUNT          265 a           66 c          113 g          199 t
ORIGIN      
        1 tgatatttaa tattacaagc aatcttaaga aattcgaaaa ggaaaaaatg attatgaaaa
       61 agaaagaaaa agaaagtatt gaaacattgt gttattctag gaatattagg aacttgtcta
      121 gctggcattg gtacaggaat agacgttgac gcagctactt actatggaaa tggtctttat
      181 tgtaacaaag aaaaatgttg ggtaaattgg ggacaatcat ggtcagaagg gttaaaacgt
      241 tggggagata atctttttgg tagttttagt ggaggacgat aatgacttca ataaacattg
      301 atcacacaaa aaaagtcata catgaattat acaatagttt aatgacacga cctaatcaga
      361 cgaaagcatt attagatatt actgatgttt tatttcaggt atataaaaaa attgatacag
      421 caaaatatcc tgaaattctt gtaaataaat tagttaatta tatttacatc gtgggttttg
      481 ataataaaat acgctttatt gataatgatg aaaaattatt aatagaatta ggggatattt
      541 ctaaaaaagc tggaattaat agtaaataca aagcaaactt tacagataaa tcgcaatttt
      601 atagttattc agaaacaatg cctcaacgat aaaatatagt ata
//
LOCUS       DACDKM010000294          634 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20834_2016
            SAMN12640370-rid9891663.denovo.307, whole genome shotgun sequence.
ACCESSION   DACDKM010000294 DACDKM010000000
VERSION     DACDKM010000294.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640370
            Sequence Read Archive: SRR10040891
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 634)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 634)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742835.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742835.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:04:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,012
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 29 of 75
            Pseudo Genes (incomplete)         :: 33 of 75
            Pseudo Genes (internal stop)      :: 19 of 75
            Pseudo Genes (multiple problems)  :: 5 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..634
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640370-rid9891663.denovo.307"
                     /isolate="E20834_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            37..180
                     /locus_tag="IV334_002946"
                     /pseudo
     CDS             37..180
                     /locus_tag="IV334_002946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326507.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein"
     gene            330..530
                     /locus_tag="IV334_002947"
     CDS             330..530
                     /locus_tag="IV334_002947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HAP8944937.1"
                     /translation="MENGTVKWFNAEKGFGFISREDGSDVFVHFSAIQGDGFKTLEEG
                     QAVTFDVEESDRGPQAANVVKA"
BASE COUNT          223 a           96 c          139 g          176 t
ORIGIN      
        1 aatgattctt ttgtaattta ctcttgataa tatgaaattc catcgcagca ggatatgaat
       61 tttaccaaaa ggatacaaaa gtgtggagaa atgatgggga tcactgtact ggaccacttg
      121 atcatcggac gcaaacgata ttttagtttg agagaagaag gaatgatgga agagaagtaa
      181 gttgctttct tgcaaaaaaa atgaagcaag tgtacaatta tattgtaatt ttgtttggga
      241 atagaagttg aaacatctgt tgcacctcta tcgtacacga gcaatgttac aatttaaatt
      301 agtgcttttg gcacgaggag gatattatta tggaaaacgg aacagtaaaa tggttcaacg
      361 ctgaaaaagg atttggattt atctcacgtg aagatggaag cgacgtattc gttcacttct
      421 cagctatcca aggtgacgga ttcaaaacat tagaagaagg tcaagcagtg actttcgacg
      481 ttgaagaatc agaccgcggc ccacaagctg caaacgttgt aaaagcttaa ttttatctgt
      541 aaatcaaatt gatggtagac atcaattttt acgagagaac aaagcgagta acaaaaatac
      601 ctgccacaag agacaatttc tcttgtggca ggta
//
LOCUS       DACDKM010000295          626 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20834_2016
            SAMN12640370-rid9891663.denovo.308, whole genome shotgun sequence.
ACCESSION   DACDKM010000295 DACDKM010000000
VERSION     DACDKM010000295.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640370
            Sequence Read Archive: SRR10040891
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 626)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 626)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742835.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742835.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:04:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,012
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 29 of 75
            Pseudo Genes (incomplete)         :: 33 of 75
            Pseudo Genes (internal stop)      :: 19 of 75
            Pseudo Genes (multiple problems)  :: 5 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..626
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640370-rid9891663.denovo.308"
                     /isolate="E20834_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            201..599
                     /gene="spxA"
                     /locus_tag="IV334_002948"
     CDS             201..599
                     /gene="spxA"
                     /locus_tag="IV334_002948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator Spx"
                     /protein_id="HAP8944938.1"
                     /translation="MLTLYTSPSCTSCRKARAWLQEHQIPFVERNIFSEPLNSSELKA
                     ILQMTEDGTEEIISTRSKVFQKLNMDLDDLPLQELLELVQNNPGLLRRPIMIDDKRLQ
                     VGFNEDEIRRFLPRDVRQLELRQAQLMAGL"
BASE COUNT          222 a           95 c          119 g          190 t
ORIGIN      
        1 tctaattttt aaatgataaa aaaagcgaga atgctggttt tactgtatat taaccttaga
       61 taactttcat ttttttaaaa aaattatgaa aaaaaggtag ctatttaaaa gttgattatg
      121 tataatagaa acagatggga gattattatg atcttctgtt acaaaaaagt gattcttatc
      181 acgaaaagga gtggtgcacg ttgttaacat tatatacttc cccaagttgt acttcctgca
      241 gaaaggctcg tgcgtggttg caagaacatc agattccatt tgttgaacgt aatatttttt
      301 cagaaccatt gaacagttct gaattaaaag cgattttaca aatgacagaa gacggaacag
      361 aagaaatcat ctcgacacgt tccaaagtat tccaaaaact gaacatggat ttagatgatc
      421 tgccgttaca agaattattg gaacttgtgc aaaacaatcc tggattgctt cgccgtccaa
      481 tcatgattga tgataaacgt ctgcaagttg gttttaatga agatgaaatt cgtcggttcc
      541 taccgcgcga tgtacgacaa ttagaattac gtcaagcaca attgatggct ggattataga
      601 aggataatac gaaacttgga aaagac
//
LOCUS       DACDKM010000296          606 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20834_2016
            SAMN12640370-rid9891663.denovo.309, whole genome shotgun sequence.
ACCESSION   DACDKM010000296 DACDKM010000000
VERSION     DACDKM010000296.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640370
            Sequence Read Archive: SRR10040891
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 606)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 606)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742835.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742835.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:04:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,012
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 29 of 75
            Pseudo Genes (incomplete)         :: 33 of 75
            Pseudo Genes (internal stop)      :: 19 of 75
            Pseudo Genes (multiple problems)  :: 5 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..606
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640370-rid9891663.denovo.309"
                     /isolate="E20834_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
BASE COUNT          182 a          108 c           84 g          232 t
ORIGIN      
        1 tttctactga gatatatttt tcctgaaaga aagccaatac cattttcata gataaagaat
       61 ccgttatata acgagataac agagctttgc tagaacattt tccattaatt tccagacata
      121 tatccaattc actgacggtt actcgcaaat ttttctctag atcgaacttt tgtaaatcat
      181 gtttcagctt atcgatcgtt tttttcaaga gaaatgtcga tagcgacaac tcttcttgaa
      241 cattcgatat tgactctgat aaatttgagc ttgcttctaa atatttaagt aatgaataac
      301 ttctgtgatc ttctttgctt aaaaaaaact cataattcat tagtcatcag ctctttctac
      361 gtatattact ttccatgcat tcaccaacta gctggggata aaactgattt cctggatccg
      421 agtcactgtt gtcagcgatt cttgtttgta taatatacat cgtcctgtct tttttttaac
      481 attttggaga gcgttttatt tggcctttct cgcgatcaaa cctgttatta gaattatatc
      541 atttactagt atgcattttt attaataaaa tggtatattt tatattgtta taaaaagagc
      601 ccaacc
//
LOCUS       DACDKM010000297          586 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20834_2016
            SAMN12640370-rid9891663.denovo.310, whole genome shotgun sequence.
ACCESSION   DACDKM010000297 DACDKM010000000
VERSION     DACDKM010000297.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640370
            Sequence Read Archive: SRR10040891
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 586)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 586)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742835.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742835.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:04:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,012
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 29 of 75
            Pseudo Genes (incomplete)         :: 33 of 75
            Pseudo Genes (internal stop)      :: 19 of 75
            Pseudo Genes (multiple problems)  :: 5 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..586
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640370-rid9891663.denovo.310"
                     /isolate="E20834_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(<1..517)
                     /locus_tag="IV334_002949"
     CDS             complement(<1..517)
                     /locus_tag="IV334_002949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8944939.1"
                     /translation="MELYTLPIIIKTEKMLKDLKQYYPLSAYEKKKLFHFLAINLLAE
                     QADSLDIASNQSLFFSDIGFYHLKGVLGEKSVIFINKTLSWLYLHDKLDQKYLRKNED
                     ETIEWLNERFIESFENQFNLLREETSQLIRRELTKIHFKLLHYTINKADEFPLDVSFF
                     RENYPAFYCYLI"
BASE COUNT          202 a           91 c           82 g          211 t
ORIGIN      
        1 ctatcaaata gcaataaaat gctggatagt tttctctaaa aaaagaaaca tcaagtggaa
       61 attcatcagc tttattaata gtgtaatgca aaagtttaaa atgtattttg gttaactctc
      121 ttcggatcaa ttggctcgtc tcttctcgca acagattaaa ctgattttca aaactttcaa
      181 tgaatcgttc attcaaccat tcgattgttt catcttcatt ttttctcaag tatttttgat
      241 ccaatttatc atgaagataa agccaagaga gtgtcttatt gataaaaata acggattttt
      301 ctcctaatac tccttttaag tggtaaaatc ctatatccga aaaaaatagc gactgattgc
      361 tagcaatatc taaagaatca gcttgttcag ccaacaggtt gattgctaaa aagtggaata
      421 atttcttttt ttcataagca gacagaggat aatactgttt taaatctttt aacatttttt
      481 ctgtttttat aattataggt agcgtataca attccacaaa acttatctct gttgagttaa
      541 aaagattata taaaaaaagc cgaatgtttt taggattccc tgtcaa
//
LOCUS       DACDKM010000298          576 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20834_2016
            SAMN12640370-rid9891663.denovo.311, whole genome shotgun sequence.
ACCESSION   DACDKM010000298 DACDKM010000000
VERSION     DACDKM010000298.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640370
            Sequence Read Archive: SRR10040891
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 576)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 576)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742835.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742835.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:04:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,012
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 29 of 75
            Pseudo Genes (incomplete)         :: 33 of 75
            Pseudo Genes (internal stop)      :: 19 of 75
            Pseudo Genes (multiple problems)  :: 5 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..576
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640370-rid9891663.denovo.311"
                     /isolate="E20834_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
BASE COUNT          212 a           77 c           93 g          194 t
ORIGIN      
        1 gatttaatcc tatatgttta gagagttcat tggcagcatg attttgcaaa gcttctcgaa
       61 ttattgttat attagaaact ttactgttca taattatgct tcttttttaa ctcaaagtcc
      121 aaagggctac agtggacttt gagttaaaaa taaaaagccc tagttatttt gaaaaataaa
      181 aaaagaacag cgtaaatgtt ttcgaaatgc tttcgcacca acatttacct gttccgtggg
      241 tttggcaccc aatgggccgt gaggggctcg aacccgcgac ccgctgatta agagtcatta
      301 atgtcaattt ttaaataaaa ttaatatgtc tctttaagta ttaaactatc actctttatc
      361 ctatgataaa tcaatgtttt ttaatattag tagtgatata ttaacatcaa ataatattaa
      421 ctaagaaaga taaggaaaga tagaaatagg gaaaaaaatc ggaaaaaatg ttggcaaaaa
      481 ttttgataaa tttactataa aaataacgtc gatgctttaa taagtatcga cgctttttac
      541 attataggaa atatattatg aatggtgttt aaaaat
//
LOCUS       DACDKM010000299          560 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20834_2016
            SAMN12640370-rid9891663.denovo.312, whole genome shotgun sequence.
ACCESSION   DACDKM010000299 DACDKM010000000
VERSION     DACDKM010000299.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640370
            Sequence Read Archive: SRR10040891
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 560)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 560)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742835.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742835.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:04:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,012
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 29 of 75
            Pseudo Genes (incomplete)         :: 33 of 75
            Pseudo Genes (internal stop)      :: 19 of 75
            Pseudo Genes (multiple problems)  :: 5 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..560
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640370-rid9891663.denovo.312"
                     /isolate="E20834_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            9..>560
                     /locus_tag="IV334_002950"
     CDS             9..>560
                     /locus_tag="IV334_002950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HAP8944940.1"
                     /translation="MSYIYLIKENYPFLTKSEKKVADFILNSGKTIIYSTMSDIKKNT
                     KVGDATIIRFCQKIGFSGFSDLKIEIAKEDFSQKKEHPTSVKYYDEIANSLTEALYST
                     IRLLDEEKLNEAIKMITQSKNIYIFGVGSSGNTSLDLENMFLRVGVQAKAVLDPHFQA
                     QVASLLTVNDLVIIFSLSGKTKDT"
BASE COUNT          204 a           72 c           92 g          192 t
ORIGIN      
        1 gagtttgtat gagttatatc tatttaatta aagaaaatta tccattttta acgaagtctg
       61 aaaaaaaagt cgctgatttt attttaaatt ctgggaaaac tattatttat agtacgatga
      121 gtgatattaa gaaaaacaca aaagttggcg atgctactat tattcgcttt tgtcaaaaaa
      181 ttggtttttc tggtttttct gatttaaaaa ttgaaattgc aaaggaagac tttagtcaaa
      241 aaaaagaaca tccgactagt gtaaagtact atgatgaaat tgctaatagt ttaacagaag
      301 ctctctattc cactattagg cttttagacg aagaaaaatt gaatgaagcc attaaaatga
      361 tcactcaatc aaaaaacata tatatatttg gagtgggatc tagtggaaat actagtcttg
      421 atttagaaaa tatgtttctt cgcgtaggag ttcaagcaaa agctgtacta gaccctcatt
      481 ttcaagccca agtcgcttct ttattaacag ttaatgattt agtaatcatt ttttctttat
      541 ctgggaaaac gaaagatact
//
LOCUS       DACDKM010000300          534 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20834_2016
            SAMN12640370-rid9891663.denovo.313, whole genome shotgun sequence.
ACCESSION   DACDKM010000300 DACDKM010000000
VERSION     DACDKM010000300.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640370
            Sequence Read Archive: SRR10040891
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 534)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 534)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742835.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742835.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:04:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,012
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 29 of 75
            Pseudo Genes (incomplete)         :: 33 of 75
            Pseudo Genes (internal stop)      :: 19 of 75
            Pseudo Genes (multiple problems)  :: 5 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..534
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640370-rid9891663.denovo.313"
                     /isolate="E20834_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..>534
                     /locus_tag="IV334_002951"
     CDS             <1..>534
                     /locus_tag="IV334_002951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012655884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HAP8944941.1"
                     /translation="SLKKHYTEPWEDEVFRVIIEYNNVPIGYGQIYKMYDELYTDYHY
                     PKTDEIVYGMDQFIGEPNYWSKGIGTRYIKLIFEFLKKERNANAVILDPHKNNPRAIR
                     AYQKSGFRIIEDLPEHELHEGKKEDCYLMEYRYDDNATNVKAMKYLIEHYFDNFKVDS
                     IEIIGSGYDSVAYLVNN"
BASE COUNT          216 a           47 c           99 g          172 t
ORIGIN      
        1 aatcattaaa aaaacattat acagagcctt gggaagatga agtttttaga gtaattattg
       61 aatataacaa tgttcctatt ggatatggac aaatatataa aatgtatgat gagttatata
      121 ctgattatca ttatccaaaa actgatgaga tagtctatgg tatggatcaa tttataggag
      181 agccaaatta ttggagtaaa ggaattggta caagatatat taaattgatt tttgaatttt
      241 tgaaaaaaga aagaaatgct aatgcagtta ttttagaccc tcataaaaat aatccaagag
      301 caataagggc ataccaaaaa tctggtttta gaattattga agatttgcca gaacatgaat
      361 tacacgaggg caaaaaagaa gattgttatt taatggaata tagatatgat gataatgcca
      421 caaatgttaa ggcaatgaaa tatttaattg agcattactt tgataatttc aaagtagata
      481 gtattgaaat aatcggtagt ggttatgata gtgtggcata tttagttaat aatg
//
LOCUS       DACDKM010000301          528 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20834_2016
            SAMN12640370-rid9891663.denovo.314, whole genome shotgun sequence.
ACCESSION   DACDKM010000301 DACDKM010000000
VERSION     DACDKM010000301.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640370
            Sequence Read Archive: SRR10040891
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 528)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 528)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742835.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742835.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:04:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,012
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 29 of 75
            Pseudo Genes (incomplete)         :: 33 of 75
            Pseudo Genes (internal stop)      :: 19 of 75
            Pseudo Genes (multiple problems)  :: 5 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..528
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640370-rid9891663.denovo.314"
                     /isolate="E20834_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(139..438)
                     /locus_tag="IV334_002952"
     CDS             complement(139..438)
                     /locus_tag="IV334_002952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HAP8944942.1"
                     /translation="MSKSEEIENMVLKLYDLVLNPEIKEKERILLIDAKTGLEKGQYY
                     PKVINNLERSLRPLAIRGELSKPVSPFYMEISTIGKFEKELGRGMASAPITFGHL"
BASE COUNT          170 a          100 c           63 g          195 t
ORIGIN      
        1 aggataaaat acaatataaa gaaacatctg tttctttatt tagaataaaa aaatacagac
       61 tctgttcaaa taaatgaacc tagcctgcat tatgagattg actatacttt ttaattcatt
      121 gagatgaata actcgttttt ataaatgccc aaaagtaatc ggtgccgaag ccattcctcg
      181 acctaattct ttttcaaatt ttcctattgt tgatatctcc atataaaacg gtgatactgg
      241 tttagacaat tctcccctga ttgctaacgg acgtaaacta cgttccaaat tatttatgac
      301 tttaggataa tactgacctt tttctaagcc tgtctttgcg tctatcaaaa gtatacgttc
      361 cttttctttg atctctggat ttaaaacgag atcatataat tttagtacca tgttttctat
      421 ttcttcactt ttagacatca cgatttcctc ccaaaaatat atttcttccc actacattct
      481 ataaacaaag tatacgctac atctagattt ttttaaatca ttactgga
//
LOCUS       DACDKM010000302          528 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20834_2016
            SAMN12640370-rid9891663.denovo.315, whole genome shotgun sequence.
ACCESSION   DACDKM010000302 DACDKM010000000
VERSION     DACDKM010000302.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640370
            Sequence Read Archive: SRR10040891
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 528)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 528)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742835.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742835.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:04:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,012
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 29 of 75
            Pseudo Genes (incomplete)         :: 33 of 75
            Pseudo Genes (internal stop)      :: 19 of 75
            Pseudo Genes (multiple problems)  :: 5 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..528
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640370-rid9891663.denovo.315"
                     /isolate="E20834_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(5..90)
                     /locus_tag="IV334_002953"
     tRNA            complement(5..90)
                     /locus_tag="IV334_002953"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(54..56),aa:Leu,seq:taa)
     gene            complement(107..178)
                     /locus_tag="IV334_002954"
     tRNA            complement(107..178)
                     /locus_tag="IV334_002954"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(144..146),aa:Gly,seq:gcc)
     gene            complement(190..262)
                     /locus_tag="IV334_002955"
     tRNA            complement(190..262)
                     /locus_tag="IV334_002955"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(227..229),aa:Thr,seq:tgt)
     gene            complement(280..361)
                     /locus_tag="IV334_002956"
     tRNA            complement(280..361)
                     /locus_tag="IV334_002956"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(325..327),aa:Leu,seq:tag)
     gene            complement(369..441)
                     /locus_tag="IV334_002957"
     tRNA            complement(369..441)
                     /locus_tag="IV334_002957"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(406..408),aa:Lys,seq:ttt)
     gene            complement(445..517)
                     /locus_tag="IV334_002958"
     tRNA            complement(445..517)
                     /locus_tag="IV334_002958"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(482..484),aa:Val,seq:tac)
BASE COUNT          135 a          152 c          128 g          113 t
ORIGIN      
        1 gaaatgccga ggaccggaat cgaaccggta cggtgatcac tcaccgcagg attttaagtc
       61 ctgtgcgtct gccagttccg ccaccccggc gcgattgctt tggcaaagcg gaaaacgggg
      121 ttcgaacccg cgacccccac cttggcaagg tggtgctcta ccactgagct atttccgcgt
      181 gtttaaagat gccggctaaa ggacttgaac cctcgaccct ctgattacaa atcagatgct
      241 ctaccaactg agctaagccg gcatgtaaaa cttaaattat gcgggtgaag ggacttgaac
      301 ccccacgccg taaggcgcta gatcctaaat ctagtgcgtc tgccaattcc gccacacccg
      361 caaaaatatg agccgtacag ggctcgaacc tgtgaccctc tgattaaaag tcagatgctc
      421 taccaactga gctaacggct catatggagg ttaacgggat cgaaccgctg accctctgct
      481 tgtaaggcag atgctctccc agctgagcta aacctccatg gataactt
//
LOCUS       DACDKM010000303          526 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20834_2016
            SAMN12640370-rid9891663.denovo.316, whole genome shotgun sequence.
ACCESSION   DACDKM010000303 DACDKM010000000
VERSION     DACDKM010000303.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640370
            Sequence Read Archive: SRR10040891
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 526)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 526)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742835.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742835.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:04:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,012
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 29 of 75
            Pseudo Genes (incomplete)         :: 33 of 75
            Pseudo Genes (internal stop)      :: 19 of 75
            Pseudo Genes (multiple problems)  :: 5 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..526
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640370-rid9891663.denovo.316"
                     /isolate="E20834_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(6..359)
                     /gene="tnpB"
                     /locus_tag="IV334_002959"
     CDS             complement(6..359)
                     /gene="tnpB"
                     /locus_tag="IV334_002959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001846105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HAP8944943.1"
                     /translation="MLYDLLQTKHIYIVCGKTDLRKGIDGLASLIQQEYQLELYEDAV
                     FLFCGNRQDRFKLLYWDGDGFLLCYKRIENGKLKWPRTKDEVRTLTNQQVRWLLEGLS
                     IDQPRAILPGKKGVF"
BASE COUNT          153 a          116 c           90 g          167 t
ORIGIN      
        1 ccagcttaaa aaactccttt ttttcctggc aaaatagctc gaggttgatc gatacttaat
       61 ccttctaaca accatcgaac ctgttggttc gttaaggttc gaacttcgtc tttcgtccga
      121 ggccatttta acttgccgtt ttctatccgc ttataacaga gaagaaaacc atcaccgtcc
      181 caatagagaa gtttgaaacg gtcttgacgg ttcccgcaaa ataaaaaaac ggcatcttcg
      241 taaagctcga gttgatactc ttgttgaata agactggcaa gaccatcaat tccttttcgc
      301 aaatctgttt ttccgcacac aatataaata tgtttggttt gaagtaaatc ataaagcatt
      361 ggacaacagt tctttcaaaa tagcttgaag aatataattg ttaataccgt tatagatcaa
      421 aacttcagaa ccattctctc ttttgatgcg catcgctaac ttggcagccg cgggtttacc
      481 tttttctggt gcactctttt cgtttcctaa ttggacagga acaatc
//
LOCUS       DACDKM010000304          515 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20834_2016
            SAMN12640370-rid9891663.denovo.317, whole genome shotgun sequence.
ACCESSION   DACDKM010000304 DACDKM010000000
VERSION     DACDKM010000304.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640370
            Sequence Read Archive: SRR10040891
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 515)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 515)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742835.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742835.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:04:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,012
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 29 of 75
            Pseudo Genes (incomplete)         :: 33 of 75
            Pseudo Genes (internal stop)      :: 19 of 75
            Pseudo Genes (multiple problems)  :: 5 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..515
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640370-rid9891663.denovo.317"
                     /isolate="E20834_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            19..>515
                     /locus_tag="IV334_002960"
     CDS             19..>515
                     /locus_tag="IV334_002960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element ISEf1 family transposase"
                     /protein_id="HAP8944944.1"
                     /translation="MNDFTTEIVQTLVTKGDLNELFRSHLEKAINTLLRTELTAFLDY
                     EKYDRTGFNSGNSRNGSYFRSIKTEYGELTLEIPRDRNGEFKQQTLPAYKRTNDTLET
                     TIIHLFEKGVTMSEIADLIEKMYGHHYTPQTMSNMTKVLTEEVNAFKSRALNDKYVAI
                     FMDAT"
BASE COUNT          188 a           93 c           86 g          148 t
ORIGIN      
        1 agaaaagagg acttccctat gaatgatttt actacagaaa ttgtgcaaac tctagtcact
       61 aaaggcgatt taaatgaatt attccgttcg cacttagaaa aagcgataaa cacactccta
      121 cggactgaat taacggcttt tttagattac gaaaaatatg atcgcactgg ttttaattca
      181 ggtaattcga gaaacggttc ttactttcga tcaatcaaaa ccgaatatgg tgaattaaca
      241 ttggaaatac ctagagatcg taatggtgag tttaaacaac aaactttacc agcctacaaa
      301 agaacaaacg atacattgga aaccactatt atccatttat tcgaaaaagg tgttacgatg
      361 tctgaaattg ctgatttgat cgaaaaaatg tacggtcatc actatactcc acaaaccatg
      421 tccaacatga ctaaagttct gactgaagaa gtaaatgcct ttaaatccag agccttaaat
      481 gataagtatg tcgctatttt tatggacgct actta
//
LOCUS       DACDKM010000305          515 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20834_2016
            SAMN12640370-rid9891663.denovo.318, whole genome shotgun sequence.
ACCESSION   DACDKM010000305 DACDKM010000000
VERSION     DACDKM010000305.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640370
            Sequence Read Archive: SRR10040891
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 515)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 515)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742835.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742835.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:04:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,012
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 29 of 75
            Pseudo Genes (incomplete)         :: 33 of 75
            Pseudo Genes (internal stop)      :: 19 of 75
            Pseudo Genes (multiple problems)  :: 5 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..515
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640370-rid9891663.denovo.318"
                     /isolate="E20834_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(107..>515)
                     /locus_tag="IV334_002961"
     CDS             complement(107..>515)
                     /locus_tag="IV334_002961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_465591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="linear amide C-N hydrolase"
                     /protein_id="HAP8944945.1"
                     /translation="GTPENHFSNQISLNVYSRGMGGLGLPGDLSSVSRFVKATFTKMN
                     AASGDSESESISQFFHILGSVEQQKGVCDTGEGKYEYTIYSSCCNVDKGIYYYRTYED
                     SQITAVNMNKEDLDRHELVSYPVIKEQQINYVN"
BASE COUNT          163 a          103 c           78 g          171 t
ORIGIN      
        1 atcatccata aaatggactt tgtctacagt ctgagacacg acctaaagat cgtgtctaat
       61 tatgactgta tcagaaaata tgataccaaa cggatctatt cattaattaa ttgacatagt
      121 ttatctgttg ctcttttatg actggataac tgaccaattc atgacgatcc aagtcttctt
      181 tattcatatt tacagcagta atttgactgt cttcatacgt acgataataa taaatccctt
      241 tatcaacgtt acagcaagaa gaataaatcg tatattcgta ttttccttct ccagtatcgc
      301 atactccttt ttgttgctcg actgagccta gtatatgaaa aaattgactg atactttcag
      361 attctgaatc acctgatgca gcattcattt tagtaaatgt ggcttttaca aatcgagaaa
      421 cagaagagag atctccaggt aagcccaatc ctcccatccc acgactataa acattcaagc
      481 tgatctggtt tgaaaaatga ttttctggtg ttcca
//
LOCUS       DACDKM010000306          508 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20834_2016
            SAMN12640370-rid9891663.denovo.319, whole genome shotgun sequence.
ACCESSION   DACDKM010000306 DACDKM010000000
VERSION     DACDKM010000306.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640370
            Sequence Read Archive: SRR10040891
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 508)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 508)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742835.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742835.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:04:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,012
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 29 of 75
            Pseudo Genes (incomplete)         :: 33 of 75
            Pseudo Genes (internal stop)      :: 19 of 75
            Pseudo Genes (multiple problems)  :: 5 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..508
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640370-rid9891663.denovo.319"
                     /isolate="E20834_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
BASE COUNT          228 a           70 c           81 g          129 t
ORIGIN      
        1 aattccttag gcagcttttg ctgtgtagaa aggaataaat aataacgaaa gttcaaaagc
       61 taatgccgac tattctagac attaaagaag acgaaaacga acgataaaga aagaaaagtt
      121 atgaataatc gtttattgaa aaaaacttga aaaaatacaa aaaattcgtt gaccaagcta
      181 aatttagttg gtatattatt acttgtccaa aagacagaag aaaatattta ttcaaaaaca
      241 taacaaaaaa gttattgaca tgttactaac gacatgataa gataactgag tcgttaaaaa
      301 agaaccactt gcgaagcttg attctatcga aaaaaacttc aaaaaagttc ttgacaaata
      361 acaaacgata agttatgata aatgagttgc cgaaagattt cgaaaaacga aatcgaaaaa
      421 aacttttaaa aaagagttga caatcgcttg tctctctgat atgatataaa agttgctacg
      481 gcagccaaaa atgaatcggg aaacactc
//
LOCUS       DACDKM010000307          485 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20834_2016
            SAMN12640370-rid9891663.denovo.320, whole genome shotgun sequence.
ACCESSION   DACDKM010000307 DACDKM010000000
VERSION     DACDKM010000307.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640370
            Sequence Read Archive: SRR10040891
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 485)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 485)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742835.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742835.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:04:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,012
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 29 of 75
            Pseudo Genes (incomplete)         :: 33 of 75
            Pseudo Genes (internal stop)      :: 19 of 75
            Pseudo Genes (multiple problems)  :: 5 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..485
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640370-rid9891663.denovo.320"
                     /isolate="E20834_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            225..>485
                     /locus_tag="IV334_002962"
     CDS             225..>485
                     /locus_tag="IV334_002962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8944946.1"
                     /translation="MKLEDKKVILKKIKRLPDQIDGYTICSFKKDRSIKAYTQNTEKG
                     IIYFLHEEGYDTQTFMFTEKKEFHKQMKKLIEKEFPRSHKLYV"
BASE COUNT          204 a           73 c           65 g          143 t
ORIGIN      
        1 caaagtatcc ctcagtaaat ataccactac cttttcttgg taatcaaatt tatttgaaaa
       61 aacatttgca ataaatcaga aaatagtggt cgtttatttg tattcgctat tctaataata
      121 aataaccact atttgattta tatatatttc agtatccttc ttgttttcat ataaccaaag
      181 gcgctatgat ttccatatga aaaaaataaa gtcaggtgaa aaaaatgaaa cttgaggata
      241 aaaaagtcat tttgaagaaa atcaaacgac taccagacca aatagatggc tatacgatct
      301 gttctttcaa aaaagacagg agtataaaag cttatacgca aaacacagaa aaaggaataa
      361 tctatttcct acatgaagaa ggttacgata cccaaacctt tatgtttact gaaaaaaaag
      421 aatttcataa acagatgaaa aaactgattg aaaaagaatt tccaagaagt cataaactct
      481 atgtg
//
LOCUS       DACDKM010000308          484 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20834_2016
            SAMN12640370-rid9891663.denovo.321, whole genome shotgun sequence.
ACCESSION   DACDKM010000308 DACDKM010000000
VERSION     DACDKM010000308.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640370
            Sequence Read Archive: SRR10040891
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 484)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 484)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742835.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742835.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:04:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,012
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 29 of 75
            Pseudo Genes (incomplete)         :: 33 of 75
            Pseudo Genes (internal stop)      :: 19 of 75
            Pseudo Genes (multiple problems)  :: 5 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..484
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640370-rid9891663.denovo.321"
                     /isolate="E20834_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(61..258)
                     /locus_tag="IV334_002963"
     CDS             complement(61..258)
                     /locus_tag="IV334_002963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HAP8944947.1"
                     /translation="MNNGTVKWFNSDKGFGFITGEDGNDVFAHFSAIQGEGFKSLDEG
                     QAVTYDIEEGQRGPQAVNIVK"
BASE COUNT          158 a           89 c           67 g          170 t
ORIGIN      
        1 aattttttct accgacataa acaaaaaaac cacctcatat gaggcggtta acgttcaaca
       61 ttattttacg atatttactg cttgaggacc acgttgtcct tcttcaatat cataagtaac
      121 tgcttggcct tcatctaaag acttaaagcc ttctccttgg attgctgaga aatgagcgaa
      181 tacgtcattt ccatcttcac cagtgatgaa accaaaacct ttatctgagt taaaccattt
      241 tactgtaccg ttattcatat atatttcctc ctgatacgta tataatacgt gttttattgc
      301 aattaatatt acttggtaaa aggagttttt atagtgtgaa tatatcgctc agattacgtt
      361 tcaaattaga ttacttattt actataacac ggatgtaatg ctaacacaag gataatcact
      421 cgatatacgg tttaaatatt attatttgtc aaattaagcg agacaaaact ttccatttac
      481 gtaa
//
LOCUS       DACDKM010000309          462 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20834_2016
            SAMN12640370-rid9891663.denovo.322, whole genome shotgun sequence.
ACCESSION   DACDKM010000309 DACDKM010000000
VERSION     DACDKM010000309.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640370
            Sequence Read Archive: SRR10040891
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 462)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 462)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742835.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742835.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:04:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,012
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 29 of 75
            Pseudo Genes (incomplete)         :: 33 of 75
            Pseudo Genes (internal stop)      :: 19 of 75
            Pseudo Genes (multiple problems)  :: 5 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..462
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640370-rid9891663.denovo.322"
                     /isolate="E20834_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(<1..430)
                     /locus_tag="IV334_002964"
     CDS             complement(<1..430)
                     /locus_tag="IV334_002964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HAP8944948.1"
                     /translation="MRKYTFVFKKKVVSDYLNNEGGYKYLAHKYQINRTLVRHWVRIY
                     NYHGWEGLVGGGKSYTTKFKLDVIEYMETNGLSIQETAKKFNIGSNRTLSKWIEQYEE
                     GGASSLESQKRGRKISMNSKLNIPKKLKDESLEEEVIRLRA"
BASE COUNT          132 a           92 c           59 g          179 t
ORIGIN      
        1 ctgctcttaa acgaataact tcttcttcaa gagactcatc tttaagtttt ttaggaatgt
       61 ttagcttgga attcatacta atttttctgc cccttttttg gctctcaagt gaagaagcac
      121 cgccttcttc atattgctct atccatttac ttagagttct atttgaaccg atattaaatt
      181 ttttagcagt ttcttggata gaaagaccat ttgtttccat atattctata acatcaagtt
      241 taaattttgt agtgtagctt ttgccacctc caaccaagcc ttcccaacca tgatagttat
      301 aaatccttac ccaatgtcta accagtgtac gatttatttg atatttatgt gcaagatatt
      361 tgtagccgcc ttcgttattt aaatagtctg aaactacttt tttcttaaaa acaaatgtat
      421 atttccgcaa aaaaagcacc ccttttaatt agatttctag tc
//
LOCUS       DACDKM010000310          460 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20834_2016
            SAMN12640370-rid9891663.denovo.323, whole genome shotgun sequence.
ACCESSION   DACDKM010000310 DACDKM010000000
VERSION     DACDKM010000310.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640370
            Sequence Read Archive: SRR10040891
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 460)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 460)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742835.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742835.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:04:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,012
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 29 of 75
            Pseudo Genes (incomplete)         :: 33 of 75
            Pseudo Genes (internal stop)      :: 19 of 75
            Pseudo Genes (multiple problems)  :: 5 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..460
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640370-rid9891663.denovo.323"
                     /isolate="E20834_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(20..91)
                     /locus_tag="IV334_002965"
     tRNA            complement(20..91)
                     /locus_tag="IV334_002965"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(56..58),aa:Glu,seq:ttc)
     gene            complement(113..200)
                     /locus_tag="IV334_002966"
     tRNA            complement(113..200)
                     /locus_tag="IV334_002966"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(164..166),aa:Ser,seq:gct)
     gene            complement(225..298)
                     /locus_tag="IV334_002967"
     tRNA            complement(225..298)
                     /locus_tag="IV334_002967"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(262..264),aa:Ile,seq:gat)
     gene            complement(312..382)
                     /locus_tag="IV334_002968"
     tRNA            complement(312..382)
                     /locus_tag="IV334_002968"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(348..350),aa:Gly,seq:tcc)
     gene            complement(384..459)
                     /locus_tag="IV334_002969"
     tRNA            complement(384..459)
                     /locus_tag="IV334_002969"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(424..426),aa:Asp,seq:gtc)
BASE COUNT          132 a          128 c          108 g           92 t
ORIGIN      
        1 taaaactata ttaaattaat gacccgtacg ggattcgaac ccgtgttacc gccgtgaaag
       61 ggcggtgtct taaccgcttg accaacgggc caggtaaaac aaaatattga aacggagaag
      121 gagggatttg aaccctcgcg ccggtttccc gacctacacc cttagcaggg gcgcctcttc
      181 agccacttga gtacttcccc aaaaccaata agattttgta ctaatgggcc taaatggact
      241 cgaaccatcg acctcacgct tatcaggcgt gcgctctaac cagctgagct ataggcccat
      301 aaaaaactaa aagcgggtga cgagaatcga actcgcgaca acagcttgga aggctgtggt
      361 tttaccacta aactacaccc gcatggcggt tccgacggga atcgaacccg cgatctcctg
      421 cgtgacaggc aggcatgtta acccctacac cacggaacca
//
LOCUS       DACDKM010000311          451 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20834_2016
            SAMN12640370-rid9891663.denovo.324, whole genome shotgun sequence.
ACCESSION   DACDKM010000311 DACDKM010000000
VERSION     DACDKM010000311.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640370
            Sequence Read Archive: SRR10040891
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 451)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 451)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742835.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742835.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:04:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,012
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 29 of 75
            Pseudo Genes (incomplete)         :: 33 of 75
            Pseudo Genes (internal stop)      :: 19 of 75
            Pseudo Genes (multiple problems)  :: 5 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..451
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640370-rid9891663.denovo.324"
                     /isolate="E20834_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(37..>451)
                     /locus_tag="IV334_002970"
     CDS             complement(37..>451)
                     /locus_tag="IV334_002970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8944949.1"
                     /translation="RKELLQDKFFLFFSYLLLLINHIPIQLISEPVKIIIDFSYGAAY
                     NQFIKKNLSLYINLNTEVIEAGKPEFPDVIITNRNNLYEESASQVVVWLDPPRAVDWG
                     NLTKILLTIQEEKYKDSKIDKQIDDFPEEIIDRIE"
BASE COUNT          137 a           83 c           75 g          156 t
ORIGIN      
        1 ttagaaaaag cagtcggact ttctaaaaga agttcactat tcgatcctgt cgatgatttc
       61 ttcaggaaaa tcgtctattt gcttgtcaat tttgctgtct ttgtatttct cttcttggat
      121 agtcaacaaa atctttgtaa gattccccca gtctactgct cttggcggat cgagccaaac
      181 cactacttga gatgcacttt cttcatatag attattccta ttcgtgatta taacatccgg
      241 gaactcaggc tttcctgcct ctatcacttc cgtgttcaga ttaatgtaca agcttaaatt
      301 tttcttgata aattgattat aggcagcacc ataagaaaaa tcgatgatga tctttactgg
      361 ttcagaaatc agttgaatgg gtatgtgatt gatcaataat aataaataac tgaaaaaaag
      421 aaaaaattta tcttgaagca actctttgcg t
//
LOCUS       DACDKM010000312          428 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20834_2016
            SAMN12640370-rid9891663.denovo.325, whole genome shotgun sequence.
ACCESSION   DACDKM010000312 DACDKM010000000
VERSION     DACDKM010000312.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640370
            Sequence Read Archive: SRR10040891
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 428)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 428)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742835.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742835.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:04:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,012
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 29 of 75
            Pseudo Genes (incomplete)         :: 33 of 75
            Pseudo Genes (internal stop)      :: 19 of 75
            Pseudo Genes (multiple problems)  :: 5 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..428
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640370-rid9891663.denovo.325"
                     /isolate="E20834_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            186..428
                     /locus_tag="IV334_002971"
                     /pseudo
     CDS             186..428
                     /locus_tag="IV334_002971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348804.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          162 a           41 c           93 g          132 t
ORIGIN      
        1 gggatttatt tttctggtta cgtgattaaa aaatccaaaa aatgagcata ggaacggtta
       61 tgattttagg gtgtgtttta atgagatatt gtgatttcat taacaagtgt atgataatct
      121 gttgttgagt tagtaaataa gtaatagagc taactctaaa ataaaactat taggaggaga
      181 tgtaggtgaa aaagtttaaa aagaaaatat atttaggatt ggttgttggg gtgagtatgt
      241 ttgggttcat ggctcatact aaacaagtaa ctgcagatac taaccaaaaa gaaaggatta
      301 tgagccaatt agatgactct caaataagta tgtacccaat gagctaattg tcagtttaaa
      361 ggctacacaa agaatttcaa cagagaaaaa taaaccgcaa aatttattgg gtgtaaaagt
      421 tatggtgg
//
LOCUS       DACDKM010000313          426 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20834_2016
            SAMN12640370-rid9891663.denovo.326, whole genome shotgun sequence.
ACCESSION   DACDKM010000313 DACDKM010000000
VERSION     DACDKM010000313.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640370
            Sequence Read Archive: SRR10040891
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 426)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 426)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742835.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742835.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:04:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,012
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 29 of 75
            Pseudo Genes (incomplete)         :: 33 of 75
            Pseudo Genes (internal stop)      :: 19 of 75
            Pseudo Genes (multiple problems)  :: 5 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..426
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640370-rid9891663.denovo.326"
                     /isolate="E20834_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(<1..132)
                     /locus_tag="IV334_002972"
     CDS             complement(<1..132)
                     /locus_tag="IV334_002972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HAP8944950.1"
                     /translation="MKLFVGIDVSSEKLDVCFLTDDNQLSILSEISVANDIEGASFIR
                     "
BASE COUNT          146 a           83 c           83 g          114 t
ORIGIN      
        1 tcgtatgaat gaagcacctt cgatgtcatt ggcaacagag atttcagata agatagacaa
       61 ttgattgtcg tctgttaaaa aacaaacatc tagtttttca gagctaacgt caataccgac
      121 aaataatttc atggaagaag gacctccttt cgatataaat tttggaaaat gcttcgatac
      181 tgtgggattt cccagaaact cactgtatag ccacaacctc gcctaataga atacggtgat
      241 aaaagagcta gaagctgcct tccaaactac tatttgaaag tctagtccat tatcgaaaca
      301 gctaatgagt tggtagtgat aacagtggtt cgggtaacag actaaacagg gtagacaaac
      361 gctacaggag gaaatagcaa attcccgttg gatcctttcc atacatccat tgtactctga
      421 gaaatc
//
LOCUS       DACDKM010000314          415 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20834_2016
            SAMN12640370-rid9891663.denovo.327, whole genome shotgun sequence.
ACCESSION   DACDKM010000314 DACDKM010000000
VERSION     DACDKM010000314.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640370
            Sequence Read Archive: SRR10040891
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 415)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 415)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742835.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742835.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:04:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,012
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 29 of 75
            Pseudo Genes (incomplete)         :: 33 of 75
            Pseudo Genes (internal stop)      :: 19 of 75
            Pseudo Genes (multiple problems)  :: 5 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..415
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640370-rid9891663.denovo.327"
                     /isolate="E20834_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(<1..339)
                     /locus_tag="IV334_002973"
     CDS             complement(<1..339)
                     /locus_tag="IV334_002973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP8944951.1"
                     /translation="MEQLKAYKFRIYPTEEQEIFFAKSFGCVRKVYNLMLDDRKKAYE
                     EVKNDSSKKMTFPTPAKYKKEFPFLKEIDSLALANAQLNLDKAYKNFFRDKSVGFPRF
                     KSKKNPVQSYT"
BASE COUNT          116 a           70 c           68 g          161 t
ORIGIN      
        1 tgtataactc tgaacaggat ttttcttact tttgaaacgt ggaaatccaa cggatttatc
       61 ccgaaaaaaa ttcttgtatg ctttatctaa attaagttgg gcattggcta aagcaaggct
      121 atcaatttct ttcaaaaacg gaaattcttt cttgtattta gctggtgtcg gaaaagtcat
      181 ttttttagaa gaatcatttt taacttcttc atacgccttt tttcggtcat caagcattag
      241 attgtagacc ttacggacac aaccaaaaga tttggcaaag aagatttctt gttcctctgt
      301 tggatagatt ctgaatttgt atgcttttag ttgttccata aagttcacct actttctatt
      361 tatgattgtt tccttgattt tggatatact tcttaatcac atcaattggt gcgcc
//
LOCUS       DACDKM010000315          408 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20834_2016
            SAMN12640370-rid9891663.denovo.328, whole genome shotgun sequence.
ACCESSION   DACDKM010000315 DACDKM010000000
VERSION     DACDKM010000315.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640370
            Sequence Read Archive: SRR10040891
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 408)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 408)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742835.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742835.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:04:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,012
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 29 of 75
            Pseudo Genes (incomplete)         :: 33 of 75
            Pseudo Genes (internal stop)      :: 19 of 75
            Pseudo Genes (multiple problems)  :: 5 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..408
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640370-rid9891663.denovo.328"
                     /isolate="E20834_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..390
                     /locus_tag="IV334_002974"
     CDS             <1..390
                     /locus_tag="IV334_002974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HAP8944952.1"
                     /translation="KDRKEICDDFKAVYQANSKEEANTFLSGMIEKWKKNYPKVTQSL
                     IENQDLLTFYDFPPSIRRTIYSTNLIESFNKQIKRYSRRKEQFQNEESLERFLVSIFD
                     TYNQKFLNRSHKGFQQVTDTLVSMFTE"
BASE COUNT          158 a           73 c           64 g          113 t
ORIGIN      
        1 aaagaccgaa aagaaatctg tgatgacttt aaggctgttt atcaagctaa ctcaaaagaa
       61 gaagcgaata ccttcttatc cggcatgatt gagaaatgga agaaaaacta tcctaaagtg
      121 acgcagtcac tcatagaaaa ccaagactta ttaacttttt atgattttcc acctagcatt
      181 cgtagaacca tttactcaac caatctaatc gagtctttca ataagcaaat taaaagatac
      241 agccgtagaa aagagcagtt tcaaaatgaa gaatcactag aacgctttct agtcagcatt
      301 tttgatacat acaatcaaaa atttctaaac agaagccata aaggttttca acaggtaacc
      361 gatacattag tttcaatgtt tactgagtaa ctaattattt tgcaggag
//
LOCUS       DACDKM010000316          381 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20834_2016
            SAMN12640370-rid9891663.denovo.330, whole genome shotgun sequence.
ACCESSION   DACDKM010000316 DACDKM010000000
VERSION     DACDKM010000316.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640370
            Sequence Read Archive: SRR10040891
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 381)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 381)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742835.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742835.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:04:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,012
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 29 of 75
            Pseudo Genes (incomplete)         :: 33 of 75
            Pseudo Genes (internal stop)      :: 19 of 75
            Pseudo Genes (multiple problems)  :: 5 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..381
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640370-rid9891663.denovo.330"
                     /isolate="E20834_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(21..94)
                     /locus_tag="IV334_002975"
     tRNA            complement(21..94)
                     /locus_tag="IV334_002975"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(58..60),aa:Ile,seq:cat)
     gene            complement(108..181)
                     /locus_tag="IV334_002976"
     tRNA            complement(108..181)
                     /locus_tag="IV334_002976"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(145..147),aa:Met,seq:cat)
     gene            complement(209..281)
                     /locus_tag="IV334_002977"
     tRNA            complement(209..281)
                     /locus_tag="IV334_002977"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(246..248),aa:Ala,seq:tgc)
     gene            complement(301..374)
                     /locus_tag="IV334_002978"
     tRNA            complement(301..374)
                     /locus_tag="IV334_002978"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(338..340),aa:Pro,seq:tgg)
BASE COUNT          103 a          108 c           90 g           80 t
ORIGIN      
        1 tgaaacttac aacgccacaa tggaccttgt aggactcgaa cctacgaccg gacggttatg
       61 agccgtctgc tctaaccaac tgagctaaag gtccaggctc ttaaaaatcg cggcggaggg
      121 gatcgaaccc ccgacctccc gggtatgaac cggacgctct agccagctga gctacaccgc
      181 gaaaaagata ttcattattt aaatgaaatg gagcctagcg ggatcgaacc gctgacctcc
      241 tgcgtgcaaa gcaggcgctc tcccagctga gctaaggccc cgtgtttaaa tttataatta
      301 tcgggaagac aggattcgaa cctgcgaccc cttggtccca aaccaagtgc tctaccaagc
      361 tgagctactt cccgtttaat t
//
LOCUS       DACDKM010000317          370 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20834_2016
            SAMN12640370-rid9891663.denovo.332, whole genome shotgun sequence.
ACCESSION   DACDKM010000317 DACDKM010000000
VERSION     DACDKM010000317.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640370
            Sequence Read Archive: SRR10040891
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 370)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 370)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742835.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742835.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:04:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,012
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 29 of 75
            Pseudo Genes (incomplete)         :: 33 of 75
            Pseudo Genes (internal stop)      :: 19 of 75
            Pseudo Genes (multiple problems)  :: 5 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..370
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640370-rid9891663.denovo.332"
                     /isolate="E20834_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(<1..>370)
                     /locus_tag="IV334_002979"
     CDS             complement(<1..>370)
                     /locus_tag="IV334_002979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8944953.1"
                     /translation="NTMGQSFTYVNGNKRAMIWSSSNLNNLFTELGTKLSLFYPGTWI
                     ATLVVLALLLCLIVGIYLSFDNKKTSIFLVILMIMGFCSRLIMGFSPTVWASGMRTYY
                     ILYVVIAILVLMAVKELMKS"
BASE COUNT          137 a           73 c           48 g          112 t
ORIGIN      
        1 atgcttttca ttagctcttt aactgccatt agtactaaga tggcgataac aacataaagt
       61 atatagtacg tccgcattcc ggaagcccat actgtgggag aaaatcccat aattaacctt
      121 gaacaaaatc ccattatcat caaaataact aaaaaaatag aagttttctt attgtcaaaa
      181 cttaaataaa ttccaacaat taaacatagt aataatgcca agactactaa agtagctatc
      241 caagtacctg gataaaaaag tgacagtttc gttcctaact ctgtaaagag attattcagg
      301 ttactactac tccaaatcat tgccctttta tttccattga cgtaagtaaa gctttgaccc
      361 attgtattac
//
LOCUS       DACDKM010000318          369 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20834_2016
            SAMN12640370-rid9891663.denovo.333, whole genome shotgun sequence.
ACCESSION   DACDKM010000318 DACDKM010000000
VERSION     DACDKM010000318.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640370
            Sequence Read Archive: SRR10040891
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 369)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 369)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742835.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742835.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:04:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,012
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 29 of 75
            Pseudo Genes (incomplete)         :: 33 of 75
            Pseudo Genes (internal stop)      :: 19 of 75
            Pseudo Genes (multiple problems)  :: 5 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..369
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640370-rid9891663.denovo.333"
                     /isolate="E20834_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            36..>369
                     /locus_tag="IV334_002980"
     CDS             36..>369
                     /locus_tag="IV334_002980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8944954.1"
                     /translation="MYEITKKHVTSSFFAIIYTTSSFRSVEIFLRGAMGELLAMSILP
                     LILLGFIKLYDSKKESWVMLAISMTLLIYTHVLSVAMTMVMIIIALIIQFYRKKKIEI
                     FLIIKLIKA"
BASE COUNT          119 a           53 c           54 g          143 t
ORIGIN      
        1 ttacgctcat tactctattt atatgtcatt atgtaatgta tgaaattacc aaaaaacatg
       61 ttactagctc ctttttcgct attatatata caacatcttc ttttaggagt gtagaaatat
      121 ttcttcgagg ggcaatgggg gaattactcg caatgtcaat acttcctctt attttactcg
      181 ggtttataaa gctttacgac tcaaaaaaag aatcttgggt tatgttggcg attagcatga
      241 ctttattaat ttacactcat gttctttctg tagctatgac tatggtaatg attataattg
      301 ctttaattat tcaattttat cggaaaaaga aaatagaaat cttcttaatt attaaattaa
      361 taaaagctg
//
LOCUS       DACDKM010000319          362 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20834_2016
            SAMN12640370-rid9891663.denovo.335, whole genome shotgun sequence.
ACCESSION   DACDKM010000319 DACDKM010000000
VERSION     DACDKM010000319.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640370
            Sequence Read Archive: SRR10040891
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 362)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 362)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742835.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742835.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:04:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,012
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 29 of 75
            Pseudo Genes (incomplete)         :: 33 of 75
            Pseudo Genes (internal stop)      :: 19 of 75
            Pseudo Genes (multiple problems)  :: 5 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..362
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640370-rid9891663.denovo.335"
                     /isolate="E20834_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(76..312)
                     /locus_tag="IV334_002981"
                     /pseudo
     CDS             complement(76..312)
                     /locus_tag="IV334_002981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611665.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          115 a           56 c           64 g          127 t
ORIGIN      
        1 atgttggtga agttcaccaa catcaaaaag tatagagccg atagaaggac tgaccttctg
       61 tctcagattt ttttgttatt tcacttgaat caattgattg atcaaaaaaa tcctgatttt
      121 catgttctca aatgatttaa atccataaga taatcgtttc agtgtcttga tatggggaat
      181 cttggcctct attttgccat tagaatacgg gtaaatcata gcattgtaaa taccttcttc
      241 atacgttaaa aggttttgaa gtttcgtgcg aagttgtgca tcaagtgttt caggaagatt
      301 ttttaataac gccgtttgta taattaagcg agacaaataa aaaagccatt tatgttacac
      361 tc
//
LOCUS       DACDKM010000320          356 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20834_2016
            SAMN12640370-rid9891663.denovo.336, whole genome shotgun sequence.
ACCESSION   DACDKM010000320 DACDKM010000000
VERSION     DACDKM010000320.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640370
            Sequence Read Archive: SRR10040891
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 356)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 356)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742835.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742835.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:04:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,012
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 29 of 75
            Pseudo Genes (incomplete)         :: 33 of 75
            Pseudo Genes (internal stop)      :: 19 of 75
            Pseudo Genes (multiple problems)  :: 5 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..356
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640370-rid9891663.denovo.336"
                     /isolate="E20834_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..>356
                     /locus_tag="IV334_002982"
     CDS             <1..>356
                     /locus_tag="IV334_002982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transcriptional antiterminator"
                     /protein_id="HAP8944955.1"
                     /translation="CIHFNEDEIAYVTLHIQSLLDRYKPDKTKVILVCSSGYGTSKLL
                     EQRINNGFADMVEIEDVLSINELQDRKVTDELVISTIPIENTNFPVIVVSPLMLGADV
                     RKVKQYLQLNEQTEDA"
BASE COUNT          135 a           49 c           67 g          105 t
ORIGIN      
        1 ctgtatacat tttaatgaag atgaaatcgc ttacgtaaca ctccatattc aatcactttt
       61 ggatcgttac aaacctgaca aaacgaaagt gatattagtt tgtagtagcg gatatggaac
      121 atcaaaatta ttagagcaaa gaataaataa cggttttgca gatatggttg agatagaaga
      181 tgtactaagc atcaatgaac tacaagatag aaaagtcact gatgaacttg ttataagcac
      241 gattccgatt gaaaatacaa attttcctgt tattgttgtt agtcctttga tgttaggggc
      301 agatgtcaga aaagtaaaac agtatctaca gttaaatgaa caaacagaag atgctt
//
LOCUS       DACDKM010000321          353 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20834_2016
            SAMN12640370-rid9891663.denovo.337, whole genome shotgun sequence.
ACCESSION   DACDKM010000321 DACDKM010000000
VERSION     DACDKM010000321.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640370
            Sequence Read Archive: SRR10040891
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 353)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 353)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742835.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742835.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:04:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,012
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 29 of 75
            Pseudo Genes (incomplete)         :: 33 of 75
            Pseudo Genes (internal stop)      :: 19 of 75
            Pseudo Genes (multiple problems)  :: 5 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..353
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640370-rid9891663.denovo.337"
                     /isolate="E20834_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(<1..>353)
                     /locus_tag="IV334_002983"
     CDS             complement(<1..>353)
                     /locus_tag="IV334_002983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-1,6-N-acetylglucosaminyltransferase"
                     /protein_id="HAP8944956.1"
                     /translation="FKIFRAIINRCLRMIKLPPKIRHHKFDLYHGSQWWALSKECLTE
                     LIQMYEQNQQDYLNFKIGMFAPDEKFFHTLFFNSSFKNKNVIGGPDLPLELKNIEETT
                     LQTSKLANIHIIDPS"
BASE COUNT          121 a           47 c           54 g          131 t
ORIGIN      
        1 atggaaggat caataatatg aatatttgct aatttcgaag tttgtaaagt agtttcttca
       61 atatttttca attcaagtgg taaatctggt cctccaataa catttttatt tttgaacgag
      121 gaattaaaaa aaagtgtatg aaaaaatttt tcatctgggg caaacatacc tattttaaaa
      181 ttcaggtagt cttgttgatt ttgttcatac atttgtatta attctgttag gcattctttc
      241 gacaaagccc accattgaga accatgatac aaatcaaatt tatggtgtct aatttttggg
      301 ggaagtttaa tcatcctcag acaacgatta attatagctc taaaaatttt aaa
//
LOCUS       DACDKM010000322         4353 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20834_2016
            SAMN12640370-rid9891663.guided.152, whole genome shotgun sequence.
ACCESSION   DACDKM010000322 DACDKM010000000
VERSION     DACDKM010000322.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640370
            Sequence Read Archive: SRR10040891
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4353)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4353)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742835.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742835.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:04:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,012
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 29 of 75
            Pseudo Genes (incomplete)         :: 33 of 75
            Pseudo Genes (internal stop)      :: 19 of 75
            Pseudo Genes (multiple problems)  :: 5 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4353
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640370-rid9891663.guided.152"
                     /isolate="E20834_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..366
                     /locus_tag="IV334_002984"
     CDS             <1..366
                     /locus_tag="IV334_002984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton antiporter"
                     /protein_id="HAP8944957.1"
                     /translation="YQKNTQLIIESLDNLTDIYDETMVQFFIQQRKLEGIKMNSGNLI
                     SSWMIQQDSLFTKKMLRGYYLERKIIDEYEVAEKITTFSANSYRRNINLLESYTMNKP
                     SDNFSFRFAFRAKSKKRVQ"
     gene            531..2417
                     /locus_tag="IV334_002985"
     CDS             531..2417
                     /locus_tag="IV334_002985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HAP8944958.1"
                     /translation="MLKELKVTDLKKTYGEKDLFDQISFLVHEKDRIGLIGTNGTGKT
                     SLLNIIAGIDSSDGDRQTVFYPTDYRIGYLSQAAEFSDELTVLQAVFQGNSPLIQTVR
                     AYEEALIELGENGEDPDVQKRYAKAEEQMNKEDAWTTDTNAKIILQKLGISELDKKIS
                     TLSGGQKKRVSLAQVLIDEPDLLLLDEPTNHLDYEAIEWLENYLKQYRGALLMVTHDR
                     YFLDRVANRIFELSFGKLYEYKGNYEAYVLEKAERDRVEVEQEEKRKRLYKQELAWMR
                     AGVQARGTKQQARINRFEDLKENLYQVNQEDDLELNLATQRLGKKVLEIKDGSYRIND
                     QTLLEHLDLLIQSRERLGITGKNGAGKSTLLNILAGRIPLDSGTMSIGETVHLAYYTQ
                     ENEEMAPDKRMIAYLQEAAEEAKTADGSQIGVAELLERFLFPRFMHGTLIGKLSGGEK
                     RRLFLLKLLIQQPNVLLLDEPTNDLDIATLTILEDYFRSFPGAVITVSHDRYFLDKVA
                     DKLLVFQGNGKQELYYGNMSSYLLKQKETQQPAEKAKPKIQSKEPAEKKKLSYMEQKE
                     WETIEDEIAELEEKISLLQEEMNHQGDNFTRLQELQNDVSETEAQLEEKMARWEYLSE
                     WVED"
     gene            2429..3376
                     /locus_tag="IV334_002986"
     CDS             2429..3376
                     /locus_tag="IV334_002986"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HAP8944959.1"
                     /translation="MEQAYLDLGRKLLEEGHRKEDRTGTGTLSLFGYQMRFDLQKGFP
                     LLTTKRVPFGLIKSELLWFLKGDTNIRYLLQNKNHIWDEWAFERYIKSPDYNGPDMTD
                     FGHRCLTDPEFNEEYKKQSKLFCEKILTDDSFAETYGDLGHIYGYQWRHWETKDGGFI
                     DQIKQVIEAIKKTPDSRRLIVSAWNPEDVPSMALPPCHTMFQFYVQEGRLSCQLYQRS
                     GDVFLGVPFNIASYALLTHLIAHETGLAVGEFVHTLGDAHLYINHIEQMKEQLSREIR
                     AFPTLVLNQEKQSVFDFEMEDIQLEGYDPHPAIKAPIAV"
     gene            3396..3923
                     /locus_tag="IV334_002987"
     CDS             3396..3923
                     /locus_tag="IV334_002987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HAP8944960.1"
                     /translation="MLAAIWAQDENGLIGKEDRLPWRLPNDLKFFKQMTEANTLVMGR
                     KTFEGMGSRPLPNRQTIVLTRDSSYKAEGVHVMHSVEDVLALEKETDGIFFIAGGSAV
                     YQEFLPFCTILYRTVIHHVFDGDAYFPPVDWSDWSLINTSQGEMDEKNRYPHQFETYQ
                     RNENPVKSGTGELTI"
     gene            complement(3986..>4353)
                     /locus_tag="IV334_002988"
     CDS             complement(3986..>4353)
                     /locus_tag="IV334_002988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HAP8944961.1"
                     /translation="YTPFCLLSIRGWLGWTLFVLIWLFAILGIIYKALTLHKRERVSK
                     VSTIIYIFMGWLCVVAAVPLYHSLGMTGVILMVAGGLSYTIGAFFYSLNSIRYMHVVW
                     HLFVMLGAGFMYFSVLFFT"
BASE COUNT         1556 a          749 c          917 g         1131 t
ORIGIN      
        1 tatcaaaaaa atactcagtt gattattgaa agtttagata acttgactga tatttatgat
       61 gaaacgatgg ttcaattttt tatccagcaa cgcaaacttg aaggaataaa aatgaactcg
      121 gggaatttga tttcttcgtg gatgatccag caagatagtc tgtttacgaa aaaaatgcta
      181 agaggttatt atcttgaaag aaaaatcatt gatgaatacg aagttgctga aaaaatcacg
      241 acgttttctg cgaacagcta tcgcaggaat atcaatttgt tagaatctta tacgatgaac
      301 aaaccatctg ataacttttc tttccgattt gcgtttcgag caaagtcaaa aaaacgagtc
      361 caataaaata aaaaggtaaa aattttcaag ttttataaaa aacaaaacat atcagaacag
      421 gatcgaatcg gtcctgttct ttttttgaga acacaaaaag aacaaatacc aaaaaaattt
      481 tcctgaagtt cactattttt atgatacaat atccaagata aggagggaac atgttgaaag
      541 aattaaaagt aactgacctc aaaaaaactt atggtgaaaa agatttgttt gaccagattt
      601 cttttttggt ccacgaaaaa gaccgtatcg ggttgattgg gacgaacggg acgggtaaaa
      661 caagtctctt aaatattatt gccggtatcg atagcagcga cggtgatcgg caaacagtgt
      721 tttatccaac tgattaccgt attggttatc tttctcaagc agctgaattt tccgatgaat
      781 taactgttct ccaagcagtc tttcaaggaa acagcccctt gatccaaact gttcgagcat
      841 atgaggaagc attgatcgaa ctaggggaaa acggagaaga ccctgatgtg caaaaacgtt
      901 atgctaaagc agaagaacaa atgaataaag aagatgcttg gactacagat accaacgcaa
      961 agattatttt gcaaaaatta ggaatcagtg aattagataa aaaaatcagt actttatcag
     1021 gtggtcagaa aaaacgagtt agtctggcgc aagtgttgat tgatgaaccc gatttactat
     1081 tactagacga accaacaaac catttagatt atgaagcgat cgagtggttg gaaaactatt
     1141 tgaaacaata ccgtggcgca ctattgatgg ttacccacga tcgttatttt ctagatagag
     1201 tagcaaaccg aatcttcgaa ctttcatttg gaaaattata cgagtataaa ggaaattatg
     1261 aagcttacgt cttagaaaaa gcagaacgtg atcgtgtaga agtagaacaa gaagaaaagc
     1321 gcaagcggct ttataaacag gaattagctt ggatgagggc tggcgtacag gcacgtggca
     1381 ccaagcagca agcacggatc aaccgtttcg aagatctaaa ggagaacttg taccaagtaa
     1441 accaagaaga tgatttagag ctgaacctag cgacacaacg tttagggaaa aaagtactgg
     1501 aaatcaaaga tggcagttac cgtataaatg accaaacact gctagaacat ttagatttat
     1561 tgattcaatc tcgggaaaga ctaggtatta ctggtaaaaa tggtgcggga aaatcgacat
     1621 tgcttaatat tctagctggc agaattcccc tggatagcgg tacaatgtct atcggtgaaa
     1681 cggttcatct tgcgtattat acacaagaaa atgaagaaat ggcaccagat aaacggatga
     1741 ttgcttactt acaagaagct gctgaagaag caaaaacagc agatggcagt caaatcggag
     1801 tagcagaatt acttgaacga tttttatttc caagatttat gcatgggaca ctgataggca
     1861 aactttcagg cggagaaaaa cgccggttat ttttgttgaa actgctgatt cagcaaccta
     1921 atgttttatt attggatgaa ccgaccaatg atttggacat agcaacattg acgattttgg
     1981 aagattattt tagaagtttt ccaggtgctg tgatcactgt ttcccatgac cgttatttct
     2041 tggacaaggt agcagataag ttattagtat tccaaggaaa cggaaaacaa gaactttatt
     2101 acggaaacat gtccagttat ttactgaagc aaaaagagac ccagcagcct gctgaaaaag
     2161 cgaaaccaaa aatacagagc aaggaacctg ctgaaaagaa gaaattatct tacatggaac
     2221 aaaaagaatg ggaaacgatt gaagatgaga ttgcagaact tgaagaaaaa atctcattgc
     2281 tccaagaaga gatgaatcat caaggagata atttcactcg attacaagaa ttgcaaaacg
     2341 acgtatctga aacagaagca caacttgaag aaaaaatggc acggtgggaa tacttgagtg
     2401 aatgggtaga agattaggag gaaaaaggat ggaacaagcg tatcttgact taggaagaaa
     2461 attgttggaa gaagggcatc gtaaagaaga cagaacaggc acaggcacat taagcttatt
     2521 cggataccaa atgcggtttg atctgcaaaa agggtttcct ttgttaacaa cgaaacgagt
     2581 accttttgga ttgatcaaaa gcgaattgct ttggttttta aaaggagata cgaatatccg
     2641 ctatctatta caaaataaaa accatatttg ggatgaatgg gcatttgaac gctacatcaa
     2701 aagtccagac tataatggac cagatatgac tgattttggc catagatgtt tgacagatcc
     2761 tgaattcaat gaagaatata aaaagcagag caagcttttt tgcgagaaga tcctaaccga
     2821 tgattccttt gctgaaacat acggcgatct tggacacatc tacggctatc aatggcgtca
     2881 ctgggaaaca aaagacggtg gctttatcga tcaaatcaaa caagtgatcg aagcaatcaa
     2941 aaagactcct gattctagaa gattgatcgt ttctgcatgg aatccggaag atgttcctag
     3001 catggctttg cctccttgtc atacgatgtt tcaattttat gttcaagagg gacggctaag
     3061 ctgccaattg tatcaacgca gtggggatgt gtttctaggc gttccgttca acattgcaag
     3121 ttatgcgttg ctgactcacc taattgcaca tgaaacaggt ttagcggttg gcgaatttgt
     3181 tcacacatta ggagatgctc atctttatat caatcatatc gaacaaatga aagaacaatt
     3241 gagccgagaa atacgcgcat ttccgacttt agttttgaat caggaaaagc agtcagtatt
     3301 tgattttgag atggaagata tccaattaga aggatatgac ccacatccgg cgatcaaagc
     3361 tcctatcgct gtttagaatc agaaaggatg agaaaatgtt agctgcaatt tgggcacagg
     3421 atgaaaacgg attgattgga aaagaagacc gtcttccttg gcgtttgcca aatgatctga
     3481 aattcttcaa acaaatgaca gaagcaaata cactagtgat gggacgaaaa acattcgaag
     3541 ggatgggtag ccgtccgttg ccgaatcgtc agacgattgt tttgacgaga gattcttcct
     3601 ataaagcaga aggcgtgcat gtcatgcata gtgtggaaga cgtgcttgct ctggaaaaag
     3661 aaacagacgg tatctttttc atcgctggag ggtcagctgt ttatcaagaa tttctgccat
     3721 tttgtacgat attataccgt acagtcatcc atcatgtatt cgatggagat gcttattttc
     3781 cacctgttga ctggtctgat tggtcactga tcaatactag tcaaggagaa atggatgaaa
     3841 aaaatcgtta tccgcatcaa tttgaaacgt atcagcgtaa tgagaatcct gtaaaaagcg
     3901 ggacaggcga attgacgata taaaaaagct atggatagac tagctgttat ttagagctgt
     3961 cccatccata gctttatttt ttttattaag taaagaataa aacagagaag tacataaaac
     4021 ctgcacctaa cataacaaac aaatgccaaa caacatgcat atagcggata gaattcaagc
     4081 tataaaagaa tgcgcctatc gtgtaagaaa gcccgcctgc taccatcaaa ataacaccag
     4141 tcattccaag agagtgataa agaggaacag cagccacgac acagagccac cccataaaaa
     4201 tatagatgat cgtagatact tttgatactc tctcccgttt atgcaacgtc aacgctttat
     4261 atataatgcc aagaatcgca aacaaccaga ttaatacaaa taaagtccaa cctagccatc
     4321 ctcggatact cagcaaacaa aaaggagtat aac
//
LOCUS       DACDKM010000323         3326 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20834_2016
            SAMN12640370-rid9891663.guided.167, whole genome shotgun sequence.
ACCESSION   DACDKM010000323 DACDKM010000000
VERSION     DACDKM010000323.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640370
            Sequence Read Archive: SRR10040891
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3326)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3326)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742835.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742835.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:04:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,012
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 29 of 75
            Pseudo Genes (incomplete)         :: 33 of 75
            Pseudo Genes (internal stop)      :: 19 of 75
            Pseudo Genes (multiple problems)  :: 5 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3326
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640370-rid9891663.guided.167"
                     /isolate="E20834_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..554
                     /locus_tag="IV334_002989"
     CDS             <1..554
                     /locus_tag="IV334_002989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HAP8944962.1"
                     /translation="NPYTGQQGAAWQFGRQKGGIDTTLAFLDKQAGKIAQQIKDFWEV
                     DLDDIPGSGAAGGIGGALFLLGAKMVSGFDLVSQITGLEKKIEQADVIITGEGRIDQQ
                     TIHGKVPYGVAVLAKKYRKPIIAICGSRDQELEGLSDFLPIVLSIHLGPIGLEEAMEH
                     RQTLEKTKILGQTLSRFFTILPK"
     gene            812..2314
                     /locus_tag="IV334_002990"
     CDS             812..2314
                     /locus_tag="IV334_002990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Eat(A)"
                     /protein_id="HAP8944963.1"
                     /translation="MSKIEIKNLTFGYDSQGTLLFEQANLNFDTQWKLGLIGRNGRGK
                     TTLLNILQNKLPYQGQVIHQQEFAYFPQQTKDKERLTYYVLNDITDFEIWEIERELQL
                     MQTDPEILWREFSTLSGGEKTKVLLALLFVDDTHFPLIDEPTNHLDISGRKQVAAYLK
                     KKKQGFIVVSHDRGFIDEVVDHVLAIEKSQLELYQGNFSIYEEQKKLRDEFEMAQNEK
                     LKKEVSRLKKTAAEKAEWSRSREGDKTKKQVGFIDTESRRVNKGAVGADAARTMKRSK
                     AIVNRMETQISEKEKLLKDIEYIDSLTMNSQASHHKRLLSVEDLQLGYENLLFEPIHF
                     TIEPHQRVAISGPNGAGKSSIIHYLLGAFNGKVIGEKYQPKHLSISYASQNYEDNRGT
                     LAEFAEKNQVDYQAFLNNLRKLGMERDVFHNKIEQMSMGQRKKVELAKSLSQPAELYT
                     WDEPLNYLDVFNQEQLEQLILNVKPAMLLVEHDQTFLDKVSTEIISLERI"
     gene            2381..2908
                     /locus_tag="IV334_002991"
     CDS             2381..2908
                     /locus_tag="IV334_002991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HAP8944964.1"
                     /translation="MLYVTRHGETTWNAQGLVCGRADVPLTEKGQMQAQKLAEKVVDL
                     PVPITKIIHSPLQRARDTAQAVADRLSLPLTVDERLVEMDFGDYDGLPSKDENFQKAR
                     LAFAVRFPNGESVLDVYARIVPLLKECIEDEENVYLLVCHNALIRVINAYFHPMPNEG
                     FFTFMVDNTELVSYE"
     gene            3119..>3326
                     /locus_tag="IV334_002992"
     CDS             3119..>3326
                     /locus_tag="IV334_002992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF871 domain-containing protein"
                     /protein_id="HAP8944965.1"
                     /translation="MFGFSIFMNDSLSEEKKQYIRQMASNGFVGIFTSMHIPEDDVSA
                     YQKRLIELGSEAKKNKLELMVDISG"
BASE COUNT         1145 a          564 c          732 g          885 t
ORIGIN      
        1 ccaatccata tacaggtcaa caaggagctg cttggcaatt tggaagacaa aaaggaggaa
       61 tagatacgac gctagctttc ttagataaac aagcaggaaa gattgctcag caaataaaag
      121 atttctggga ggtggattta gatgatattc caggaagtgg agcagcaggc ggtattggtg
      181 gagccctttt tttgttggga gcgaagatgg tttctggttt cgatttagtc agccaaatca
      241 ctggattgga aaaaaagatc gaacaagcag atgtcatcat tacaggagaa gggcggatcg
      301 atcagcaaac gatccacgga aaagttccat acggtgtagc agtgcttgca aaaaaatatc
      361 gaaaaccaat aatcgctatt tgcggaagta gagaccaaga gctagaagga ctttctgatt
      421 ttctcccaat tgtcttaagt attcatttgg gtccaatagg tttagaagaa gcaatggaac
      481 acaggcaaac attagaaaaa acaaaaatct taggacagac acttagcaga tttttcacca
      541 tcctcccgaa gtagttgaca aaatcatttt ctttatgcta tcgtaatttc aaatatagga
      601 caaagtgagg gatccgtgca ttgaaaaatt aagtcgattg atgaaagctg cattcagact
      661 gtaccttgtg gagacaagga agcagtgtgc agattttcgg gattggcttg gttttttcga
      721 tgtatgggtc tttttgtgtg tatccaaccc ttgcatcctc tttagccagt tctgaaaata
      781 ggactggtct ttttttacga ggtgatggaa aatgtctaaa atcgaaataa aaaatctgac
      841 attcggctac gacagccaag gcacattatt atttgaacaa gcaaatctaa attttgacac
      901 acaatggaaa ctaggactta tcggacgaaa cggtcgagga aagacaactt tactgaatat
      961 tctacaaaac aaactacctt atcaagggca agtaatccat cagcaagaat ttgcctattt
     1021 cccgcaacag acaaaagata aagaacgttt aacctattac gtgttaaatg atattacgga
     1081 ttttgagata tgggaaatcg aaagagagct ccaattgatg caaacagatc ctgaaatctt
     1141 atggagagaa ttcagcacac tatcgggggg agagaagaca aaagtcctac tggcactttt
     1201 atttgtggat gacactcatt tcccgttaat cgatgaacca acgaatcatt tggatatctc
     1261 tggtagaaaa caagtagcgg cttatttgaa aaagaaaaaa caaggcttca tcgtggtcag
     1321 ccatgaccgg ggatttatcg atgaagtagt ggaccatgtt ttagcaatcg aaaaaagtca
     1381 actggaactt tatcaaggga atttctctat ctatgaagaa cagaaaaaac ttcgtgatga
     1441 atttgaaatg gctcaaaatg aaaaattgaa aaaagaagtc agtaggctaa agaaaacagc
     1501 agctgaaaaa gccgaatggt ctcgttcccg agaaggagat aaaacaaaga aacaagtcgg
     1561 attcatcgat actgaatcta gacgagtgaa taaaggagca gtgggtgctg atgctgcacg
     1621 gacgatgaaa cgatccaaag caatcgtgaa tcggatggag acccagatca gcgagaaaga
     1681 aaaactatta aaagatatcg aatatatcga ttctttgacg atgaatagcc aagcgtctca
     1741 ccataagcga cttttaagcg tagaagatct tcaattaggg tatgaaaatc tgttattcga
     1801 gccaattcat tttacaatcg agcctcatca gcgggtggcg atttcaggtc ctaacggtgc
     1861 aggaaagtca tccattatcc attatcttct gggggcattc aacggcaagg ttataggaga
     1921 aaaataccag ccaaaacatc tgagcattag ttatgcaagc caaaattatg aagacaatcg
     1981 aggaacgttg gcggaatttg cagagaaaaa ccaagtagac taccaagcat ttttgaacaa
     2041 cctccgaaag cttgggatgg aaagagatgt ttttcataac aagatcgagc agatgagtat
     2101 gggccaacgg aaaaaagtgg aattggctaa atctttatca cagccagctg aactatatac
     2161 atgggatgaa ccattgaatt atttggatgt cttcaatcaa gaacaattag aacaactgat
     2221 cttgaacgtg aaacctgcca tgttactagt ggaacatgat caaaccttcc tggataaagt
     2281 atctactgag attatttctc ttgagagaat ctaacccttg taatctcaag aacaacaagg
     2341 tatggttaag gggaactttg ccaacgaagg agagaatcac atgctttatg taacgagaca
     2401 cggagaaaca acttggaacg cacaaggatt agtttgcgga cgggcagatg tgccgctgac
     2461 tgaaaaagga cagatgcagg cacagaaact agcagaaaag gtagtagatt tgcctgttcc
     2521 aatcacgaaa atcatccatt cccctcttca acgagcaaga gatacagcac aagcagttgc
     2581 tgatcgtctc agcttgccgc tgaccgttga tgaacgattg gtagaaatgg attttgggga
     2641 ttatgatgga ctgccaagca aagatgagaa ctttcaaaaa gcacgtttag cgttcgctgt
     2701 acgattccca aatggggagt cagtcttaga tgtgtatgca cggatcgttc ctttattgaa
     2761 agaatgcatc gaagacgaag aaaatgtcta tctgttagtt tgccataatg ccttgataag
     2821 agtgatcaat gcttattttc atccgatgcc taatgagggg ttctttacat tcatggtaga
     2881 taatacagaa ttggtttctt atgagtaatt atgtttttgt gttatttaaa taaaatattt
     2941 caaggcactg cttttgctta gcagtgcctt ttttaggtta tgacaattct tttgataata
     3001 taaactcaac ctttagaaat gaaatttaat tattttaata aaagtattga aattttgttt
     3061 ctttagatat atactctagt catagaaatg aagacggata cagaaaaaga gggataatat
     3121 gtttggcttt tcaatattta tgaacgattc tttgtcagaa gagaagaaac agtatatccg
     3181 ccaaatggca tccaatggct ttgtcggtat tttcacttcc atgcatatac cagaagatga
     3241 tgtttcggca tatcaaaagc gattgatcga actaggaagc gaagcgaaaa agaataaact
     3301 agaattgatg gtcgatattt caggag
//
LOCUS       DACDKM010000324         2928 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20834_2016
            SAMN12640370-rid9891663.guided.177, whole genome shotgun sequence.
ACCESSION   DACDKM010000324 DACDKM010000000
VERSION     DACDKM010000324.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640370
            Sequence Read Archive: SRR10040891
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2928)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2928)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742835.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742835.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:04:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,012
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 29 of 75
            Pseudo Genes (incomplete)         :: 33 of 75
            Pseudo Genes (internal stop)      :: 19 of 75
            Pseudo Genes (multiple problems)  :: 5 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2928
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640370-rid9891663.guided.177"
                     /isolate="E20834_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(70..567)
                     /gene="dfrG"
                     /locus_tag="IV334_002993"
     CDS             complement(70..567)
                     /gene="dfrG"
                     /locus_tag="IV334_002993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000868795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrG"
                     /protein_id="HAP8944966.1"
                     /translation="MKVSLIAAMDKNRVIGKENDIPWRIPKDWEYVKNTTKGHPIILG
                     RKNLESIGRALPDRRNIILTRDKGFTFNGCEIVHSIEDVFELCKNEEEIFIFGGEQIY
                     NLFFPYVEKMYITKIHHEFEGDTFFPEVNYEEWNEVFAQKGIKNDKNPYNYYFHVYER
                     KNLLS"
     gene            797..1969
                     /locus_tag="IV334_002994"
     CDS             797..1969
                     /locus_tag="IV334_002994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HAP8944967.1"
                     /translation="MKYILSFCTPKLNYLLAYIRLYSGVLHLRDSVRVSEKEKIKVTE
                     MYTSINGELCKIDRAYSGEIVILRNEFLKLNSVLGDTKLLPQRKKIENPHPLLQTTVE
                     PSKPEQREMLLDALLEISDSDPLLRYYVDSTTHEIILSFLGKVQMEVISALLQEKYHV
                     EIELKEPTVIYMERPLKNAEYTIHIEVPPNPFWASIGLSVSPLPLGSGMQYESSVSLG
                     YLNQSFQNAVMEGIRYGCEQGLYGWNVTDCKICFKYGLYYSPVSTPADFRMLTPIVLE
                     QAFRKAGTELLEPYLSFKVYAPQEYLSRAYNDAPKYCANIVNTQLKNNEVIIIGEIPA
                     RCIQDYRNDLTFFTNGLSVCLAELKGYQVTTGEPVCQTRRLNSRIDKVRYMFNKIT"
     gene            2067..2255
                     /locus_tag="IV334_002995"
     CDS             2067..2255
                     /locus_tag="IV334_002995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011988843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAP8944968.1"
                     /translation="MINYKWILCPICGNKTRLRIRVDTILKNFPLYCPKCKNETLINV
                     QKMNIITIKEPDAKTQSR"
     gene            complement(2313..2666)
                     /locus_tag="IV334_002996"
     CDS             complement(2313..2666)
                     /locus_tag="IV334_002996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001227349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP8944969.1"
                     /translation="MRKKEDKYDFRAVGLAIKEARMKRGLTREQVGTMIEIDPRYLTN
                     IENKGQHPSTQVLYDLVSLLHVSIDEFFLPTDNLIKSTRRLQVEKYMDSFTDKELSLM
                     EALAKGINEARNIEG"
BASE COUNT          951 a          500 c          467 g         1010 t
ORIGIN      
        1 aaaagagctt ggagtaatcc caagctcttg tgctttatct ttactgttct tcttcaatta
       61 acgcacccgt taactcaata agttttttct ttcatataca tgaaaatagt agttatacgg
      121 atttttatca ttctttatcc ctttttgggc aaatacctca ttccattcct cataattcac
      181 ttctggaaaa aaagtatctc cttcgaattc atgatgtatt tttgtgatgt acattttctc
      241 aacataaggg aaaaacaaat tataaatctg ttctcctccg aaaataaaaa tttcttcttc
      301 gtttttacat aactcaaaaa catcttctat tgaatgaaca atttcacaac cattaaaggt
      361 aaacccctta tctctcgtca gaataatatt tcttctgtca ggtaaggctc ttccgattga
      421 ttcaaggttc ttcctaccta atattatcgg atgtcccttt gtagtatttt taacatattc
      481 ccagtccttg ggaatcctcc aaggaatgtc attctctttg ccaatcactc tattcttatc
      541 catcgcagca atcaaagaaa ctttcattta taacacagcc tttttaacta atttaccatc
      601 taatttctta tttctatcaa aaaacctttt ctcattatcc tgcccgttag ttgaataagc
      661 aattttttct ataagttata ttatcataat accacaaaat cacaaatttt cattcaatta
      721 tcattttcaa ttgcatttta taaatcattt tgtatgtaat ttggtatgta agttaaaatg
      781 aaaaaagtag tccattttga agtacatttt gtcattttgt actccaaaac tgaactactt
      841 acttgcatat atacgccttt atagtggagt actacattta cgagattcgg ttagagtatc
      901 agaaaaagaa aaaataaaag ttacagaaat gtatacttca ataaatggtg aattatgtaa
      961 gattgataga gcttattctg gagaaattgt tattttgcga aatgagtttt tgaagttaaa
     1021 tagtgttctt ggagatacaa aactattgcc acagagaaaa aagattgaaa atccgcaccc
     1081 tctactacaa acaactgttg aaccgagtaa acctgaacag agagaaatgt tgcttgatgc
     1141 ccttttggaa atctcagata gtgatccgct tctacgatat tacgtggatt ctacgacaca
     1201 tgaaattata ctttctttct tagggaaagt acaaatggaa gtgattagtg cactgttgca
     1261 agaaaagtat catgtggaga tagaactaaa agagcctaca gtcatttata tggagagacc
     1321 gttaaaaaat gcagaatata ccattcacat cgaagtgccg ccaaatcctt tctgggcttc
     1381 cattggttta tctgtatcac cgcttccgtt gggaagtgga atgcagtatg agagctcggt
     1441 ttctcttgga tacttaaatc aatcatttca aaatgcagtt atggaaggga tacgctatgg
     1501 ttgcgaacaa ggattatatg gttggaatgt gacggattgt aaaatctgtt ttaagtacgg
     1561 tttatactat agccctgtta gtactccagc agattttcgg atgcttactc ctattgtact
     1621 ggagcaagcc tttagaaaag ctggaacaga attgttagag ccatatctta gttttaaagt
     1681 ttatgcacca caggaatatc tttcacgggc atataacgat gctcccaaat attgtgcaaa
     1741 tatcgtaaat actcaactga aaaataatga ggtcattatt attggagaaa ttcctgctcg
     1801 atgtattcaa gattatcgca atgatttaac tttttttaca aatgggctta gtgtttgttt
     1861 agcagagcta aaaggatatc aggttaccac tggcgaacct gtttgccaga cccgtcgtct
     1921 aaatagtcgg atagataaag taagatatat gttcaataaa ataacttagt gcgttttatg
     1981 ttgttatata aatatggttt cttattaaat aagatgaaat attctttaat atagatttga
     2041 attaaagtgg aaaggaggag attgttatta taaactacaa gtggatattg tgtcctattt
     2101 gtggaaataa aacaagacta cgaatacgag tggatactat acttaaaaat ttccctttat
     2161 actgccccaa atgtaagaac gaaactttaa ttaatgttca aaaaatgaat ataataacaa
     2221 tcaaagagcc agacgccaag acgcagagcc gataatttga gaaatgaaac tctcatctta
     2281 tcggctcttt ttgtttatct gaattttact gactagcctt caatatttct tgcttcattg
     2341 ataccttttg ctagtgcttc cattaaggat agttctttgt ctgtaaagct atccatgtat
     2401 ttttctacct gtaaccttcg agtgctttta atcaaattat ctgtaggtaa gaaaaattca
     2461 tcaatagata catggagtaa tgatacaaga tcatacagca cttgtgtact tgggtgttgt
     2521 cctttgtttt ctatatttgt taaataacgt gggtcaattt caatcattgt tcccacttgt
     2581 tcacgagtga gaccccgttt cattcgagcc tctttaatcg ctaaaccaac ggctctaaaa
     2641 tcgtatttat cttctttttt acgcatacta aatcacctct atacatttta ctgttcctat
     2701 ataaccataa acaggtatag aaaaacatgt tatatagttt atgagttcct atagaacact
     2761 gtggaattta ttaatgcgat gaatccaatt tcaaatagaa aggtcgctga ttttatttac
     2821 ctatacaact ttataaaatt tagtcatact cctagaaagt agataaaatt ggcgacttaa
     2881 tggagggaaa agtcgccatg agaaagataa tctgtcatct agttctct
//
LOCUS       DACDKM010000325         2178 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20834_2016
            SAMN12640370-rid9891663.guided.203, whole genome shotgun sequence.
ACCESSION   DACDKM010000325 DACDKM010000000
VERSION     DACDKM010000325.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640370
            Sequence Read Archive: SRR10040891
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2178)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2178)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742835.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742835.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:04:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,012
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 29 of 75
            Pseudo Genes (incomplete)         :: 33 of 75
            Pseudo Genes (internal stop)      :: 19 of 75
            Pseudo Genes (multiple problems)  :: 5 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2178
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640370-rid9891663.guided.203"
                     /isolate="E20834_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..180
                     /locus_tag="IV334_002997"
     CDS             <1..180
                     /locus_tag="IV334_002997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HAP8944970.1"
                     /translation="RDHGYETNPIGGFEADQLAEAFGLDKDRYVPVIILSIGKAVEEG
                     YESVRLAPEKITMFE"
     gene            534..2012
                     /gene="msr"
                     /locus_tag="IV334_002998"
     CDS             534..2012
                     /gene="msr"
                     /locus_tag="IV334_002998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Msr family ABC-F type ribosomal protection
                     protein"
                     /protein_id="HAP8944971.1"
                     /translation="MENLAVNITNLQVSFGNQLELSIDSLRVYQQDRIGIIGENGVGK
                     STLLKLIAGELFPDHGKIQTEITFNYLPQLTYLAEAKDLNLELASHFQLRLEETSERK
                     WSGGEERKIELIRLLSSYEQGMLLDEPTTHLDRKSIDRLIEELRYYYGTLVFVSHDRY
                     FLDELASKIWEVKDGEIREFSGNYSAYLTQKELEKKTQLREAESIMKEKKRLEKSIQE
                     KKKQAEKLEKVSSKKKKQQIRLDRLSSSKQKDSVQKAIQKNAKTLERRLQKIGETTKP
                     QQMKQIRFPVPKSLELHSRYPIMGQNVQLERSGRTLLVNGDFQFSLGKKIAIVGENGS
                     GKTTLLEHIRKQGEGILLSPKVSFQVYQQKGYQMTSEESIIRFVMRQTEFSESLVRSL
                     LNHLGFAQETLTKPLCTLSGGEATRLTIALLFTKPSNVLLLDEPTNFIDMATIEALEK
                     LMQIYPGTILFTSHDSYFVERTADEVYEIKGQKIKKVLTRNF"
     gene            2059..>2178
                     /locus_tag="IV334_002999"
     CDS             2059..>2178
                     /locus_tag="IV334_002999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016186386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2798 domain-containing protein"
                     /protein_id="HAP8944972.1"
                     /translation="MPTNKKEGIIFTTMMCFLMVLGMSAYNLWLHQDLHLSSLL"
BASE COUNT          754 a          346 c          465 g          613 t
ORIGIN      
        1 cgtgatcatg gctatgaaac aaatccaatt ggtggattcg aagcagatca gttggcagag
       61 gcatttggtt tggataaaga ccgttatgtt ccagttatta ttttatcaat aggaaaagcg
      121 gtagaagaag gttacgagtc tgttcgttta gcaccagaaa agatcactat gttcgaataa
      181 aaggttttca gttactattc agtagctatt gctttttttg aatcgtgtgt ggtaacctaa
      241 taaaaaagga ggtcggaaaa atgactgcat cgatgaaatt acgtttcgaa cttttgaata
      301 acaactaaat aaattcaaaa gacatgtata aacaacaatt tcatggatgg agtatgtcca
      361 ttttttcgaa cgacagtaga ataggtgaaa gctatgacag atttggtgat ttagtcatag
      421 ttatttcgtt cagagtagga aaagatgtga tgcagttttt ttgcaaacag atttttctgc
      481 tcttttttag tatcgataat tgttgtttta catgaaatag gagtgtaaaa catatggaaa
      541 atttagcagt aaatataaca aatctacaag tcagctttgg caaccagcta gaactatcta
      601 ttgattctct tcgtgtctat cagcaagatc gtatagggat cataggagaa aatggtgtcg
      661 gtaaatcgac tttgctcaaa ctaatagccg gtgaactttt tcccgatcat gggaaaatcc
      721 aaacagagat caccttcaac tacctgcctc aattaaccta tctcgctgaa gcaaaggacc
      781 taaatttgga attagccagt catttccagt taagactgga agaaacttca gagcggaaat
      841 ggagcggagg ggaagaacga aagatcgagt tgatacgtct tctttcttct tatgaacaag
      901 ggatgcttct agacgagcca acaacccatc tagatagaaa aagtattgat cgactgattg
      961 aagagcttcg ttattattat ggtacgctgg tttttgttag tcatgatcgc tattttctag
     1021 atgagctagc atcgaaaatc tgggaagtaa aagacggaga aatccgagag ttttcgggga
     1081 attatagtgc ctatctcact caaaaggaat tggagaaaaa gactcagcta cgagaagcag
     1141 agtcgatcat gaaagagaaa aaacgattgg aaaaatcgat ccaagaaaag aaaaaacaag
     1201 cggaaaagtt ggaaaaagtg tccagtaaaa agaaaaagca acaaatcaga ctggatcggt
     1261 tgtcttcctc taaacaaaaa gacagtgtac aaaaagccat ccaaaagaat gcgaaaacat
     1321 tagagagaag actccaaaaa ataggagaaa caaccaagcc gcagcagatg aaacaaatcc
     1381 gttttccagt accaaaatct cttgaactcc acagccgcta tcccatcatg ggacaaaatg
     1441 tccaattgga aagaagtggg agaacattac tggtaaatgg tgattttcag ttttctttag
     1501 gtaaaaaaat cgcgattgtc ggtgaaaatg gttcaggtaa gacaacctta ttagaacata
     1561 tccgcaaaca aggagaagga atccttctct ctccgaaagt aagctttcaa gtatatcagc
     1621 aaaagggtta tcaaatgaca tctgaagaat ccatcattcg ttttgtcatg agacaaacag
     1681 agttttcaga atcgcttgtc cgtagtttgc tgaatcactt agggtttgct caggaaactt
     1741 tgacgaaacc gttatgtaca ttaagtgggg gagaagcgac ccgtctgacg attgctttgc
     1801 tttttactaa gccaagtaat gtgttgctgt tagatgaacc gactaatttt attgatatgg
     1861 caacgatcga agctttagag aagctaatgc aaatatatcc gggaacgatt ttgtttactt
     1921 ctcatgattc ttactttgtc gagcgtacgg ctgatgaagt ttatgaaata aaagggcaga
     1981 aaataaaaaa agtacttacg agaaattttt aaatgtgcta cgatgatttt cgtgacaaat
     2041 ttaaaggagt ggtacagatt gcctacaaat aaaaaagaag gaataatttt tacaacaatg
     2101 atgtgttttt tgatggtctt ggggatgagc gcgtataatt tatggctgca tcaagatctt
     2161 catttatcaa gtttgttg
//
LOCUS       DACDKM010000326         1647 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20834_2016
            SAMN12640370-rid9891663.guided.222, whole genome shotgun sequence.
ACCESSION   DACDKM010000326 DACDKM010000000
VERSION     DACDKM010000326.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640370
            Sequence Read Archive: SRR10040891
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1647)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1647)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742835.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742835.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:04:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,012
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 29 of 75
            Pseudo Genes (incomplete)         :: 33 of 75
            Pseudo Genes (internal stop)      :: 19 of 75
            Pseudo Genes (multiple problems)  :: 5 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1647
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640370-rid9891663.guided.222"
                     /isolate="E20834_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            15..797
                     /locus_tag="IV334_003000"
     CDS             15..797
                     /locus_tag="IV334_003000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000067268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside nucleotidyltransferase ANT(9)-Ia"
                     /protein_id="HAP8944973.1"
                     /translation="MSNLINGKIPNQAIQTLKIVKDLFGSSIVGVYLFGSAVNGGLRI
                     NSDVDVLVVVNHSLPQLTRKKLTERLMTISGKIGNTDSVRPLEVTVINRSEVVPWQYP
                     PKREFIYGEWLRGEFENGQIQEPSYDPDLAIVLAQARKNSISLFGPDSSSILVSVPLT
                     DIRRAIKDSLPELIEGIKGDERNVILTLARMWQTVTTGEITSKDVAAEWAIPLLPKEH
                     VTLLDIARKGYRGECDDKWEGLYSKVKALVKYMKNSIETSLN"
     gene            complement(923..>1647)
                     /gene="erm(A)"
                     /locus_tag="IV334_003001"
     CDS             complement(923..>1647)
                     /gene="erm(A)"
                     /locus_tag="IV334_003001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001072197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(A)"
                     /protein_id="HAP8944974.1"
                     /translation="KNPKDTQNFITSKKHVKEILNHTNISKQDNVIEIGSGKGHFTKE
                     LVKMSRSVTAIEIDGGLCQVTKEAVNPSENIKVIQTDILKFSFPKHINYKIYGNIPYN
                     ISTDIVKRITFESQAKYSYLIVEKGFAKRLQNLQRALGLLLMVEMDIKMLKKVPPLYF
                     HPKPSVDSVLIVLERHQPLISKKDYKKYRSFVYKWVNREYRVLFTKNQFRQALKHANV
                     TNINKLSKEQFLSIFNSYKLFH"
BASE COUNT          528 a          252 c          308 g          559 t
ORIGIN      
        1 tacgaggtga aatcatgagc aatttgatta acggaaaaat accaaatcaa gcgattcaaa
       61 cattaaaaat cgtaaaagat ttatttggaa gttcaatagt tggagtatat ctatttggtt
      121 cagcagtaaa tggtggttta cgcattaaca gcgatgtaga tgttctagtc gtcgtgaatc
      181 atagtttacc tcaattaact cgaaaaaaac taacagaaag actaatgact atatcaggaa
      241 agattggaaa tacggattct gttagaccac ttgaagttac ggttataaat aggagtgaag
      301 ttgtcccttg gcaatatcct ccaaaaagag aatttatata cggtgagtgg ctcaggggtg
      361 aatttgagaa tggacaaatt caggaaccaa gctatgatcc tgatttggct attgttttag
      421 cacaagcaag aaagaatagt atttctctat ttggtcctga ttcttcaagt atacttgtct
      481 ccgtaccttt gacagatatt cgaagagcaa ttaaggattc tttgccagaa ctaattgagg
      541 ggataaaagg tgatgagcgt aatgtaattt taaccctagc tcgaatgtgg caaacagtga
      601 ctactggtga aattacctcg aaagatgtcg ctgcagaatg ggctatacct cttttaccta
      661 aagagcatgt aactttactg gatatagcta gaaaaggcta tcggggagag tgtgatgata
      721 agtgggaagg actatattca aaggtgaaag cactcgttaa gtatatgaaa aattctatag
      781 aaacttctct caattaggct aattttattg caataacagg tgcttacttt taaaactact
      841 gatttattga taaatattga acaatttttg ggaagaataa agcgtcctct tgtgaaatta
      901 gagaacgctt tattacttta atttagtgaa acaatttgta actattgaaa atagaaagaa
      961 attgttcctt cgatagttta ttaatattag tgacatttgc atgcttcaaa gcctgtcgga
     1021 attggttttt agtgaaaaga acacgatatt cacggtttac ccacttataa acaaaagatc
     1081 gatacttttt gtagtccttc tttgaaatca atggttgatg tcgttcaaga acaatcaata
     1141 cagagtctac acttggctta ggatgaaaat atagtggtgg tacttttttg agcattttta
     1201 tatccatctc caccattaat agtaaaccca aagctcgttg cagattttgc aatcttttcg
     1261 caaatccctt ctcaacgata agatagctat atttagcctg actttcaaag gtaattcttt
     1321 tgacaatatc cgtactgatg ttataaggaa tattaccata tatcttatag tttatatgtt
     1381 ttgggaagga aaattttaga atatccgttt gaatcacttt tatattctca gaggggttta
     1441 ccgcttcttt agtcacttga cataagcctc catcaatttc tatagcagta actgatcgac
     1501 tcattttgac tagctctttg gtaaaatgtc cttttcctga tccgatttct attacgttgt
     1561 cttgtttact gatattcgtg tgattcaata tttcttttac atgcttttta gaagtaataa
     1621 aattttgcgt gtctttaggg tttttct
//
LOCUS       DACDKM010000327         1349 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20834_2016
            SAMN12640370-rid9891663.guided.241, whole genome shotgun sequence.
ACCESSION   DACDKM010000327 DACDKM010000000
VERSION     DACDKM010000327.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640370
            Sequence Read Archive: SRR10040891
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1349)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1349)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742835.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742835.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:04:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,012
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 29 of 75
            Pseudo Genes (incomplete)         :: 33 of 75
            Pseudo Genes (internal stop)      :: 19 of 75
            Pseudo Genes (multiple problems)  :: 5 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1349
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640370-rid9891663.guided.241"
                     /isolate="E20834_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            46..297
                     /locus_tag="IV334_003002"
                     /pseudo
     CDS             46..297
                     /locus_tag="IV334_003002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627290.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
     gene            390..1184
                     /locus_tag="IV334_003003"
                     /note="aph(3')-IIIa"
     CDS             390..1184
                     /locus_tag="IV334_003003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase
                     APH(3')-IIIa"
                     /protein_id="HAP8944975.1"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLK
                     MTDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEY
                     EDEQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWE
                     EDTPFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKW
                     YDIAFCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
BASE COUNT          424 a          234 c          322 g          369 t
ORIGIN      
        1 cattttcctc taggaaaaaa ttaactagca ccacgggtta tcttttatat agaagatatc
       61 gccgtatgta aggatttcag ggggcaaggc ataggcagcg cgcttatcaa tatatctata
      121 gaatgggcaa agcataaaaa cttgcatgga ctaatgcttg aaacccagga caataacctt
      181 atagcttgta aattctatca taattgtggt ttcaaaatcg gctccgtcga tactatgtta
      241 tacgccaact ttgaaaacaa ctttgaaaaa gctgttttct ggtatttaag gttttagaat
      301 gcaaggaaca gtgaattgga gttcgtcttg ttataattag cttcttgggg tatctttaaa
      361 tactgtagaa aagaggaagg aaataataaa tggctaaaat gagaatatca ccggaattga
      421 aaaaactgat cgaaaaatac cgctgcgtaa aagatacgga aggaatgtct cctgctaagg
      481 tatataagct ggtgggagaa aatgaaaacc tatatttaaa aatgacggac agccggtata
      541 aagggaccac ctatgatgtg gaacgggaaa aggacatgat gctatggctg gaaggaaagc
      601 tgcctgttcc aaaggtcctg cactttgaac ggcatgatgg ctggagcaat ctgctcatga
      661 gtgaggccga tggcgtcctt tgctcggaag agtatgaaga tgaacaaagc cctgaaaaga
      721 ttatcgagct gtatgcggag tgcatcaggc tctttcactc catcgacata tcggattgtc
      781 cctatacgaa tagcttagac agccgcttag ccgaattgga ttacttactg aataacgatc
      841 tggccgatgt ggattgcgaa aactgggaag aagacactcc atttaaagat ccgcgcgagc
      901 tgtatgattt tttaaagacg gaaaagcccg aagaggaact tgtcttttcc cacggcgacc
      961 tgggagacag caacatcttt gtgaaagatg gcaaagtaag tggctttatt gatcttggga
     1021 gaagcggcag ggcggacaag tggtatgaca ttgccttctg cgtccggtcg atcagggagg
     1081 atatcgggga agaacagtat gtcgagctat tttttgactt actggggatc aagcctgatt
     1141 gggagaaaat aaaatattat attttactgg atgaattgtt ttagtaccta gatttagatg
     1201 tctaaaaagc tttaactaca agctttttag acatctaatc ttttctgaag tacatccgca
     1261 actgtccata ctctgatgtt ttatatcttt tctaaaagtt cgctagatag gggtcccgag
     1321 cgcctacgag gaatttgtat cgataagaa
//
LOCUS       DACDKM010000328         1098 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20834_2016
            SAMN12640370-rid9891663.guided.261, whole genome shotgun sequence.
ACCESSION   DACDKM010000328 DACDKM010000000
VERSION     DACDKM010000328.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640370
            Sequence Read Archive: SRR10040891
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1098)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1098)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742835.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742835.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:04:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,012
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 29 of 75
            Pseudo Genes (incomplete)         :: 33 of 75
            Pseudo Genes (internal stop)      :: 19 of 75
            Pseudo Genes (multiple problems)  :: 5 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1098
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640370-rid9891663.guided.261"
                     /isolate="E20834_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..71
                     /locus_tag="IV334_003004"
     CDS             <1..71
                     /locus_tag="IV334_003004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="23S rRNA methyltransferase attenuation leader
                     peptide"
                     /protein_id="HAP8944976.1"
                     /translation="MRYVDKTSTVLKQTKNSDYADK"
     gene            196..933
                     /gene="erm(B)"
                     /locus_tag="IV334_003005"
     CDS             196..933
                     /gene="erm(B)"
                     /locus_tag="IV334_003005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004292767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(B)"
                     /protein_id="HAP8944977.1"
                     /translation="MNKNIKYSQNFLTSEKVLNQIIKQLNLKETDTVYEIGTGKGHLT
                     TKLAKISKQVTSIELDSHLFNLSSEKLKLNTRVTLIHQDILQFQFPNKQRYKIVGNIP
                     YHLSTQIIKKVVFESRASDIYLIVEEGFYKRTLDIHRTLGLLLHTQVSIQQLLKLPAE
                     CFHPKPKVNSVLIKLTRHTTDVPDKYWKLYTYFVSKWVNREYRQLFTKNQFHQAMKHA
                     KVNNLSTVTYEQVLSIFNSYLLFNGRK"
BASE COUNT          421 a          182 c          163 g          332 t
ORIGIN      
        1 aaatgcgtta tgtagataaa acatctactg ttttgaaaca gactaaaaac agtgattacg
       61 cagataaata aatacgttag attaattcct accagtgact aatcttatga ctttttaaac
      121 agataactaa aattacaaac aaatcgttta acttctgtat ttgtttatag atgtaatcac
      181 ttcaggagtg ataacatgaa caaaaatata aaatattctc aaaacttttt aacgagtgaa
      241 aaagtactca accaaataat aaaacaattg aatttaaaag aaaccgatac cgtttacgaa
      301 attggaacag gtaaagggca tttaacgacg aaactggcta aaataagtaa acaggtaacg
      361 tctattgaat tagacagtca tctattcaac ttatcgtcag aaaaattaaa actgaatact
      421 cgtgtcactt taattcacca agatattcta cagtttcaat tccctaacaa acagaggtat
      481 aaaattgttg ggaatattcc ttaccattta agcacacaaa ttattaaaaa agtggttttt
      541 gaaagccgtg cgtctgacat ctatctgatt gttgaagaag gattctacaa gcgtaccttg
      601 gatattcacc gaacactagg gttgctcttg cacactcaag tctcgattca gcaattgctt
      661 aagctgccag cggaatgctt tcatcctaaa ccaaaagtaa acagtgtctt aataaaactt
      721 acccgccata ccacagatgt tccagataaa tattggaagc tatatacgta ctttgtttca
      781 aaatgggtca atcgagaata tcgtcaactg tttactaaaa atcagtttca tcaagcaatg
      841 aaacacgcca aagtaaacaa tttaagtacc gttacttatg agcaagtatt gtctattttt
      901 aatagttatc tattatttaa cgggaggaaa taattctatg agtcgctttt gtaaatttgg
      961 aaagttacac gttactaaag ggaatgtaga taaattatta ggtatagggc acctctaata
     1021 actaccaatt aattcacctc aaaatgaaat tagaaaaaaa cagcgaaatt tttaaatcta
     1081 tttcttatcg atacaaat
//
LOCUS       DACDKM010000329         1055 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20834_2016
            SAMN12640370-rid9891663.guided.265, whole genome shotgun sequence.
ACCESSION   DACDKM010000329 DACDKM010000000
VERSION     DACDKM010000329.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640370
            Sequence Read Archive: SRR10040891
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1055)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1055)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742835.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742835.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:04:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,012
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 29 of 75
            Pseudo Genes (incomplete)         :: 33 of 75
            Pseudo Genes (internal stop)      :: 19 of 75
            Pseudo Genes (multiple problems)  :: 5 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1055
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640370-rid9891663.guided.265"
                     /isolate="E20834_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            340..888
                     /gene="aac(6')"
                     /locus_tag="IV334_003006"
     CDS             340..888
                     /gene="aac(6')"
                     /locus_tag="IV334_003006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside N-acetyltransferase AAC(6')-Ii"
                     /protein_id="HAP8944978.1"
                     /translation="MIISEFDRNNPVLKDQLSDLLRLTWPEEYGDSSAEEVEEMMNPE
                     RIAVAAVDQDELVGFIGAIPQYGITGWELHPLVVESSRRKNQIGTRLVNYLEKEVASR
                     GGITIYLGTDDLDHGTTLSQTDLYEHTFDKVASIQNLREHPYEFYEKLGYKIVGVLPN
                     ANGWDKPDIWMAKTIIPRPNSQ"
BASE COUNT          369 a          154 c          212 g          320 t
ORIGIN      
        1 ttctaccgat tccctttgct tttttgtaat tatctaaaaa ttctaaaaat taagattatt
       61 atatgaaaac aggttttatt tgtcaattca taattggttt tttttaaagc aaaagataat
      121 ctatatcatg atgaacaaac agagtttttt cctccaattg atggctttcc tgatctttcc
      181 tattttgaat aaagtgtttc tgtataattt ttttatttta taagtaagat aaataatgaa
      241 agaatagaga tactgattgg tagctaaaga tttttgagcg agaacatttc gttttttcaa
      301 aaggactgtt agaataagca agaacaaggg ggaagacgta tgataatcag tgaatttgac
      361 cgtaataatc cagtattgaa agatcagctt tctgatttac tgagactgac ttggccggaa
      421 gaatatggag acagctcggc agaagaagta gaagaaatga tgaatccaga acgaatcgcg
      481 gtagcagcgg tagaccaaga tgagttagta ggatttattg gtgcaatccc tcaatacggt
      541 atcacaggtt gggaattgca tccattagtt gtagaaagct cccgacgaaa gaaccaaata
      601 ggtactcgat tagtcaatta cttagaaaaa gaagtagctt ccagaggagg aatcacgatt
      661 tatttaggta cggatgattt agaccatgga acaacgttaa gtcaaacgga cctgtatgag
      721 catacatttg ataaagtggc ttccatccag aaccttcgtg aacatccgta tgaattctat
      781 gaaaaattag gttataaaat cgtaggtgtc ttaccaaatg caaatggctg ggacaaaccg
      841 gatatttgga tggcaaaaac gattattcct cgaccaaatt ctcaataata aaaagaggtt
      901 gtgacagaag taatcagctt caagaaataa gaaggaattt acgaaaatta cttcttgaat
      961 ttttgtgaaa ttccaactta tttccgaaga agctactttt ggaacaccgt gtaccagagg
     1021 ttgtgaaaaa gtcgcttagt ttcgagtagt aagaa
//
LOCUS       DACDKM010000330         1018 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20834_2016
            SAMN12640370-rid9891663.guided.268, whole genome shotgun sequence.
ACCESSION   DACDKM010000330 DACDKM010000000
VERSION     DACDKM010000330.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640370
            Sequence Read Archive: SRR10040891
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1018)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1018)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742835.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742835.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:04:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,012
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 29 of 75
            Pseudo Genes (incomplete)         :: 33 of 75
            Pseudo Genes (internal stop)      :: 19 of 75
            Pseudo Genes (multiple problems)  :: 5 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1018
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640370-rid9891663.guided.268"
                     /isolate="E20834_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            35..766
                     /gene="erm(A)"
                     /locus_tag="IV334_003007"
     CDS             35..766
                     /gene="erm(A)"
                     /locus_tag="IV334_003007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001072197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(A)"
                     /protein_id="HAP8944979.1"
                     /translation="MNKKNPKDTQNFITSKKHVKEILNHTNISKQDNVIEIGSGKGHF
                     TKELVKMSRSVTAIEIDGGLCQVTKEAVNPSENIKVIQTDILKFSFPKHINYKIYGNI
                     PYNISTDIVKRITFESQAKYSYLIVEKGFAKRLQNLQRALGLLLMVEMDIKMLKKVPP
                     LYFHPKPSVDSVLIVLERHQPLISKKDYKKYRSFVYKWVNREYRVLFTKNQFRQALKH
                     ANVTNINKLSKEQFLSIFNSYKLFH"
     gene            complement(892..>1018)
                     /locus_tag="IV334_003008"
     CDS             complement(892..>1018)
                     /locus_tag="IV334_003008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000067268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aminoglycoside nucleotidyltransferase ANT9"
                     /protein_id="HAP8944980.1"
                     /translation="TLLDIARKGYRGECDDKWEGLYSKVKALVKYMKNSIETSLN"
BASE COUNT          379 a          170 c          157 g          312 t
ORIGIN      
        1 tcagttcatt ataaccagta aggagaaggt tataatgaac aagaaaaacc ctaaagacac
       61 gcaaaatttt attacttcta aaaagcatgt aaaagaaata ttgaatcaca cgaatatcag
      121 taaacaagac aacgtaatag aaatcggatc aggaaaagga cattttacca aagagctagt
      181 caaaatgagt cgatcagtta ctgctataga aattgatgga ggcttatgtc aagtgactaa
      241 agaagcggta aacccctctg agaatataaa agtgattcaa acggatattc taaaattttc
      301 cttcccaaaa catataaact ataagatata tggtaatatt ccttataaca tcagtacgga
      361 tattgtcaaa agaattacct ttgaaagtca ggctaaatat agctatctta tcgttgagaa
      421 gggatttgcg aaaagattgc aaaatctgca acgagctttg ggtttactat taatggtgga
      481 gatggatata aaaatgctca aaaaagtacc accactatat tttcatccta agccaagtgt
      541 agactctgta ttgattgttc ttgaacgaca tcaaccattg atttcaaaga aggactacaa
      601 aaagtatcga tcttttgttt ataagtgggt aaaccgtgaa tatcgtgttc ttttcactaa
      661 aaaccaattc cgacaggctt tgaagcatgc aaatgtcact aatattaata aactatcgaa
      721 ggaacaattt ctttctattt tcaatagtta caaattgttt cactaaatta aagtaataaa
      781 gcgttctcta atttcacaag aggacgcttt attcttccca aaaattgttc aatatttatc
      841 aataaatcag tagttttaaa agtaagcacc tgttattgca ataaaattag cctaattgag
      901 agaagtttct atagaatttt tcatatactt aacgagtgct ttcacctttg aatatagtcc
      961 ttcccactta tcatcacact ctccccgata gccttttcta gctatatcca gtaaagtt
//
LOCUS       DACDKM010000331         1018 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20834_2016
            SAMN12640370-rid9891663.guided.269, whole genome shotgun sequence.
ACCESSION   DACDKM010000331 DACDKM010000000
VERSION     DACDKM010000331.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640370
            Sequence Read Archive: SRR10040891
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1018)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1018)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742835.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742835.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:04:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,012
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 29 of 75
            Pseudo Genes (incomplete)         :: 33 of 75
            Pseudo Genes (internal stop)      :: 19 of 75
            Pseudo Genes (multiple problems)  :: 5 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1018
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640370-rid9891663.guided.269"
                     /isolate="E20834_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            35..766
                     /gene="erm(A)"
                     /locus_tag="IV334_003009"
     CDS             35..766
                     /gene="erm(A)"
                     /locus_tag="IV334_003009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001072197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(A)"
                     /protein_id="HAP8944981.1"
                     /translation="MNQKNPKDTQNFITSKKHVKEILNHTNISKQDNVIEIGSGKGHF
                     TKELVKMSRSVTAIEIDGGLCQVTKEAVNPSENIKVIQTDILKFSFPKHINYKIYGNI
                     PYNISTDIVKRITFESQAKYSYLIVEKGFAKRLQNLQRALGLLLMVEMDIKMLKKVPP
                     LYFHPKPSVDSVLIVLERHQPLISKKDYKKYRSFVYKWVNREYRVLFTKNQFRQALKH
                     ANVTNINKLSKEQFLSIFNSYKLFH"
     gene            complement(892..>1018)
                     /locus_tag="IV334_003010"
     CDS             complement(892..>1018)
                     /locus_tag="IV334_003010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000067268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aminoglycoside nucleotidyltransferase ANT9"
                     /protein_id="HAP8944982.1"
                     /translation="TLLDIARKGYRGECDDKWEGLYSKVKALVKYMKNSIETSLN"
BASE COUNT          378 a          171 c          157 g          312 t
ORIGIN      
        1 tcagttcatt ataaccagta aggagaaggt tataatgaac cagaaaaacc ctaaagacac
       61 gcaaaatttt attacttcta aaaagcatgt aaaagaaata ttgaatcaca cgaatatcag
      121 taaacaagac aacgtaatag aaatcggatc aggaaaagga cattttacca aagagctagt
      181 caaaatgagt cgatcagtta ctgctataga aattgatgga ggcttatgtc aagtgactaa
      241 agaagcggta aacccctctg agaatataaa agtgattcaa acggatattc taaaattttc
      301 cttcccaaaa catataaact ataagatata tggtaatatt ccttataaca tcagtacgga
      361 tattgtcaaa agaattacct ttgaaagtca ggctaaatat agctatctta tcgttgagaa
      421 gggatttgcg aaaagattgc aaaatctgca acgagctttg ggtttactat taatggtgga
      481 gatggatata aaaatgctca aaaaagtacc accactatat tttcatccta agccaagtgt
      541 agactctgta ttgattgttc ttgaacgaca tcaaccattg atttcaaaga aggactacaa
      601 aaagtatcga tcttttgttt ataagtgggt aaaccgtgaa tatcgtgttc ttttcactaa
      661 aaaccaattc cgacaggctt tgaagcatgc aaatgtcact aatattaata aactatcgaa
      721 ggaacaattt ctttctattt tcaatagtta caaattgttt cactaaatta aagtaataaa
      781 gcgttctcta atttcacaag aggacgcttt attcttccca aaaattgttc aatatttatc
      841 aataaatcag tagttttaaa agtaagcacc tgttattgca ataaaattag cctaattgag
      901 agaagtttct atagaatttt tcatatactt aacgagtgct ttcacctttg aatatagtcc
      961 ttcccactta tcatcacact ctccccgata gccttttcta gctatatcca gtaaagtt
//
LOCUS       DACDKM010000332          870 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20834_2016
            SAMN12640370-rid9891663.guided.280, whole genome shotgun sequence.
ACCESSION   DACDKM010000332 DACDKM010000000
VERSION     DACDKM010000332.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640370
            Sequence Read Archive: SRR10040891
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 870)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 870)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742835.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742835.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:04:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,012
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 29 of 75
            Pseudo Genes (incomplete)         :: 33 of 75
            Pseudo Genes (internal stop)      :: 19 of 75
            Pseudo Genes (multiple problems)  :: 5 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..870
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640370-rid9891663.guided.280"
                     /isolate="E20834_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..185
                     /locus_tag="IV334_003011"
     CDS             <1..185
                     /locus_tag="IV334_003011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000393259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP8944983.1"
                     /translation="YGSKLLSFIKEYSKEIGCSEMFLITDKGNPRACHVYEKLGGKND
                     YKDEIVYVYDYEKGDK"
     gene            186..>870
                     /gene="aac(6')-Ie"
                     /locus_tag="IV334_003012"
     CDS             186..>870
                     /gene="aac(6')-Ie"
                     /locus_tag="IV334_003012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012655884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside N-acetyltransferase AAC(6')-Ie"
                     /protein_id="HAP8944984.1"
                     /translation="MNIVENEICIRTLIDDDFPLMLKWLTDERVLEFYDGRDKKYTLE
                     SLKKHYTEPWEDEVFRVIIEYNNVPIGYGQIYKMYDELYTDYHYPKTDEIVYGMDQFI
                     GEPNYWSKGIGTRYIKLIFEFLKKERNANAVILDPHKNNPRAIRAYQKSGFRIIEDLP
                     EHELHEGKKEDCYLMEYRYDDNATNVKAMKYLIEHYFDNFKVDSIEIIGSGYDSVAYL
                     VNNEYIFKTK"
BASE COUNT          357 a           64 c          157 g          292 t
ORIGIN      
        1 gttatggttc aaaattatta tcttttatta aggaatattc taaagagatt ggttgttctg
       61 aaatgttttt aataactgat aaaggtaatc ctagagcttg ccatgtatat gaaaaattag
      121 gtggtaaaaa tgattataaa gatgaaatag tatatgtata tgattatgaa aaaggtgata
      181 aataaatgaa tatagttgaa aatgaaatat gtataagaac tttaatagat gatgattttc
      241 ctttgatgtt aaaatggtta actgatgaaa gagtattaga attttatgat ggtagagata
      301 aaaaatatac attagaatca ttaaaaaaac attatacaga gccttgggaa gatgaagttt
      361 ttagagtaat tattgaatat aacaatgttc ctattggata tggacaaata tataaaatgt
      421 atgatgagtt atatactgat tatcattatc caaaaactga tgagatagtc tatggtatgg
      481 atcaatttat aggagagcca aattattgga gtaaaggaat tggtacaaga tatattaaat
      541 tgatttttga atttttgaaa aaagaaagaa atgctaatgc agttatttta gaccctcata
      601 aaaataatcc aagagcaata agggcatacc aaaaatctgg ttttagaatt attgaagatt
      661 tgccagaaca tgaattacac gagggcaaaa aagaagattg ttatttaatg gaatatagat
      721 atgatgataa tgccacaaat gttaaggcaa tgaaatattt aattgagcat tactttgata
      781 atttcaaagt agatagtatt gaaataatcg gtagtggtta tgatagtgtg gcatatttag
      841 ttaataatga atacattttt aaaacaaaat
//
